LOCUS       DAOSLI010000001       133117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_122_length_133117_cov_23.977048, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000001 DAOSLI010000000
VERSION     DAOSLI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 133117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 133117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..133117
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_133117_cov_23.977048"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1322)
                     /locus_tag="PLF67_00005"
     CDS             complement(252..1322)
                     /locus_tag="PLF67_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE88424.1"
                     /translation="MNKDPFKEYIKESEPDKRNKGYAWHTAIGLQAVDGLKTSEYLTH
                     TALRNIEGEISFEEANAFLQAYHEENPSRNAEDRTEEADKVAARIAELLSERAFSFTP
                     NEYLSIHRKLFTGIYSHAGRIRDHAITKKEWVLDGATVLYGSATELGATLDYEISEER
                     TFSYKNLSMDEIIHHLAVCISRLWQIHVFGEGNTRTTAVFFIKYLRTLGFDMTNDVFA
                     ENAWFFRNSLVRANYNDLKNGIHETTEFLELFLRNLLLDENHPLHNRTLHISGTWKET
                     EKPDIEGLKADIEPVFQPKTVNHILTLREAFPGRAIFGRSDVMRAIKIKPSRASELLK
                     ELVEHEVIEPVSGHGKGKYRFR"
     gene            complement(1433..2653)
                     /locus_tag="PLF67_00010"
     CDS             complement(1433..2653)
                     /locus_tag="PLF67_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE88425.1"
                     /translation="MLIQRKRLLDALVRYRDKDLVKIITGVRRSGKSVLLNELFYHYL
                     IDDGVQADHILKIAFDMKKWEHLRDSGHLYSYLLSQFADDDKYYLFLDEIQMVDGFEE
                     VLNEIRSEYNTDIYVTGSNSKLLSSDINTIFRGRGMEIKCFPLSFQEFHSFRGGDTRE
                     SLDEYMFFGSLPYTVTEPDTTNKRDYLDMVANTVITRDMIDRYGIRNESLFRGVIRLL
                     CSSIGSAVSANNIANILKSNGYKTVDNETVDRYLQHVTDAFLFYKADRYDIKGKVYLK
                     TRQKYYVCDMGLRNALILYRQLELSRAIENIIYVELLRRGYAVDIGKNRDKEIDFIAR
                     DMEGRRNYIQVSFSIENPSVKERELSSFHGLDDGYKKILITMDRTPFSNLEHGYRLLT
                     VLDFLLSDDSIERA"
     gene            complement(2753..3880)
                     /locus_tag="PLF67_00015"
     CDS             complement(2753..3880)
                     /locus_tag="PLF67_00015"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOE88426.1"
                     /translation="MRVRIEKVIQGGEGLAVPEDGRRIFVPAVLPGELVEVALVESKS
                     GFSRAELLEILEPSPARIGERCPYWGVCGGCDFQYAIHAEQIRIKEGIIRENFTRIGK
                     IDEINLLPTEVGKPWEYRCRSRFHVDLRSRKAGFLGRNSNELVPIDSCPVLTPKLDSL
                     LKEPDLLIESAYRLQQRNHLVEVGALASGEQISLLGREIALPVLDRTLYASSEVFFQS
                     NEVLLEPLVSYVQAHVVGDTVIDLYSGVGTFSAFVEAKRVIAVERSKRCLAFAKKNAP
                     HSDFYTGNVANLASVQKGRIDTVIVDPPRVGFDRNLHEKIAGWGTRRIIYVSCNSVTL
                     ARDVGTLVDLGFTLDSVKQFDFYPQTFHHEAVAILSKGDAD"
     gene            complement(3864..5597)
                     /locus_tag="PLF67_00020"
     CDS             complement(3864..5597)
                     /locus_tag="PLF67_00020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOE88427.1"
                     /translation="MKSLPRWDLTPIYPGPQSAEFLADIELVRTKSAELEARLADEKS
                     DLQINIEAFELILDHMGNLGSYAHALVSTDTTNAVYLKAFNQVEDLVLVLKPLSVRFM
                     RHLAKREDEIKDRGADDPYAYVLQGLLIEQKHLMSDELEEFAADLARSSWDAFSRLQQ
                     SLGSSISAEWEDGRMKTVVELRNMATDGDRAIRKRAFEKELELLEIHEEAFAAALNGV
                     KGATISLNARRGWGSPLERSIFQSRIDSEILSALIATLEEAVPLFRSYLKKKAELLGL
                     ENLAFYDLFAPVGKSAKRYSYEDAQAFIVEHFSRFSEAMGKFAQNAFEKNWIDPEPRK
                     GKVGGAYDTSFPVAKESRILSNFDYTYSGVSTIAHELGHAYHDSKVMQRGGLLANYPM
                     PLAETASIFCEGLIFRGALEEADEEERIALIEAFLQDSTQVCLDILSRFYFEEEVFKR
                     RKEGDITASELNEMMLDAQKRTYGPALDVYHPYMWAVKSHYYRPEFSHYNYPYAFGQL
                     FGLGLSKRSEDDPSAFVEQYDALLSVSGMKSVRDVGLEAGIDLGDRSFWQEGIEVIAR
                     YVEEFKSASPH"
     gene            complement(5607..6959)
                     /locus_tag="PLF67_00025"
     CDS             complement(5607..6959)
                     /locus_tag="PLF67_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE88428.1"
                     /translation="MNVLIVDDEINIRQLMSRYLKLEGIQSSEAENGLSAQRMLREQG
                     FDACIIDLRMPGMNGLELIQWIRSEGLRMPIIMISAHGEISDAVTALKEGAQDYIVKP
                     FDPEELVLRLKKLVELQNLRNVVESESRQADSEGELFIGESPQIRQIKKVIEKIADTT
                     SMVLITGESGTGKEVVARRIHQTSPVADGPFVAINIGGVPENLIESELFGYEKGAFTG
                     AVSRKIGMFELAGGGTLFLDEIGDMPLSLQVKILRVLQERKIIRLGGTTELPINARII
                     AATNKDLEERVRNGSFREDLFYRLNVVRIHIPPLRQRREDIPLLAAGILKRLNREMGH
                     TVTGISADALNRITEHDFYGNVRELENILERAMIFATEAEIQESDLDLRHSVSLHSQA
                     QQPQELPQSEAKSLKEAEIEAIVHALHRWEGNRTRAAEELGISRRTLINKIAEYNLSL
                     "
     gene            complement(6956..8269)
                     /locus_tag="PLF67_00030"
     CDS             complement(6956..8269)
                     /locus_tag="PLF67_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE88429.1"
                     /translation="MRRSRKRLMIGDGREGVFVISSLIFGFLLLLGLLVFLTSALYER
                     EELRLQNEAERAFNSVFLALQDSNAKALRTMAEENVSGVGVYSSMGRKVMSLGKVPMT
                     IPAELFTTRDGFVRTGRATYNKATQMVEYIRFSRLTILLETGQLTLTEQGLLPTPIDF
                     PDLLYLSLDGSAYHHRLMVIVVLGFFSGFVIIGLFIAVLTIYLKNRSYRQTLAKQESL
                     VNLGQAARTLAHEIKNPLSAVTIQVALLKKTLSEEHKEDLQVIEGEVNRLTQLTNKIS
                     DFLRNPLGQPEVVNVDEFFEMLIKAFDMPIAYTSERPLSILIDPDRMRSIFENILKNA
                     VESNEGEDAQVEVAVSTGKKHVIHITVKDRGVGIKKSDMKKIFDPFYTTKIQGSGIGL
                     SISQQFVRARGGSISLRGREGGGTIVEVILPEAIRPLRKHKESEG"
     gene            complement(8279..9460)
                     /locus_tag="PLF67_00035"
     CDS             complement(8279..9460)
                     /locus_tag="PLF67_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88430.1"
                     /translation="MVIENIKLAFNAMRGNKMRTALSLLGIVIGVASVVAILTIGDSA
                     SQSIKDAIAIGGLDMVTIYPSPGQRNTGIFNEEFSSMLRRDVEGIDVVLPQNSSIARI
                     RVSQEATDASVVGVLSDYGDALNLEYTQGSFFDAMDNINRRQVAVLGSSVAEELFPEQ
                     EAVGRYISLFRNQAKNYLVVGILKSKDPTFNLSYDDSVFIPYNTYSQRFIRTTNVGAF
                     VVKVTDGFDTIAVSDRITEYLDDIVGTDGYMLFSPATLAEMADQITGTFSTFLALIAA
                     ISLLVGGIGIMNIMLVSVAERTREIGVRKAIGASPSVIRGQFLIEALTLTILGGFLGI
                     VLGSLLSFAVTNLMDWSLHLSYASFILALGFSMFVGVFFGWYPAVKASKLDPIDALNY
                     E"
     gene            complement(9462..10133)
                     /locus_tag="PLF67_00040"
     CDS             complement(9462..10133)
                     /locus_tag="PLF67_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE88431.1"
                     /translation="MSDVIALRNVSRYYVMGEFIVKALDGVSITIKKGEFTSIMGPSG
                     SGKSTMMNLIGCLDTPTDGQILIDGEDTSGMNEAELAFIRNQKVGFVFQQFNLLGKLT
                     ALENVVTPLLYAGYRPRERRRMAINALESVGLADRMYHRPNELSGGQKQRVAIARALV
                     NNPSILLADEPTGALDTRTGNQIMELFDEINSEGRTVILVTHDRELGLKAKRQIYLRD
                     GKIEV"
     gene            complement(10126..11250)
                     /locus_tag="PLF67_00045"
     CDS             complement(10126..11250)
                     /locus_tag="PLF67_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE88432.1"
                     /translation="MFEMQRSDTATETLVKNQFKKKQRRRRARRFFTWIFILALIAGG
                     LYLYTFYKENNRFPWAEERAPISFVRETDSEVYESIFTQTIDLSSFVEPNDIQQVILR
                     ATGTVTDVYVKEGDSVRKGDVLIALDDINERFDVAQSQDALQAAILTGASDREIELAE
                     LRLTNAKNKLDYTKAYANFDGVVASVKVSEGDYFTAGQTAMTIVDRSTLKATVEIDEI
                     DMQYVELGQKAVLYFDSYTDGSIEGYVSYIPMLGRYTNQGIGVMDVELTIESPPARIV
                     PGYTFKGTITSEGETALILLPQSAVTTSRGSSSVLKRNEDGTTTRVPVTVKYLGEGIS
                     QLLSGDLKAGDTVVTRRTDTSNPFASMGGNMNMMVEIPDE"
     gene            complement(11262..12731)
                     /locus_tag="PLF67_00050"
     CDS             complement(11262..12731)
                     /locus_tag="PLF67_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88433.1"
                     /translation="MHRPYTRRVGVVAAVLLLAALPLSAIPLDDLLKQAEQNSSSMKN
                     LEITRSNVLLQQAATKIDDGVKVSVNSGELKYSNPIDTKNNWNVSRQRISLSGSSVEV
                     TLPNDGKTAFSFSVDPFSYSQAGSSWNYLEDPSLSMSHTITYGKTQDSLKDLNTRQTE
                     MMANSSYESAKLTFANTLYSQIGALVNNEKSIKSTTQKLTDAKKELSDKLVLGQVKKG
                     SLVYQAQDQAIRLTEGTLESLQASRDLLIRQFETIMGVPYPDAITDIREPDLSFAMNA
                     EGSTSVASKRIEVQKAKETLALRIAQYTNRTLVAGGNISYANSQINTNLGPMTQNSLT
                     LGGSAALAGKSFTVQGAVGGTYDFDKGTLTPVVAISGTWSNDPAFAKDTLEIRQLENE
                     VLLTEIAYNNAVDEYIQSSIEVQNGIASWQLEYALLLQTIRYNKDLLKQQRELFENGL
                     VTKKQVEDAAFTVELDEYDLKAMLLKGLQLENEIKRLMM"
     gene            complement(12817..13521)
                     /locus_tag="PLF67_00055"
     CDS             complement(12817..13521)
                     /locus_tag="PLF67_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE88434.1"
                     /translation="MSEVVLITGGARRLGSVMAQSLLQKGYRVVVHYNTSEAAAFELQ
                     RIHGAKIIQGDLSIPEAVEPLFREALTAYGVIDHVINNASCFERGTIGELTLEAYQRM
                     MNLHTTAPLFLSKALYHHLLERGRTGSIINIVDTRVSKPTASRVGYYLSKSALLEQTK
                     VLAVALGPTLRVNAVSPGAVLSNGDEAYFKTMQQILPLRRTGTAEAVAEAALYLLEAD
                     FVSGIELAVDGGQKLL"
     gene            13610..15538
                     /gene="ftsH"
                     /locus_tag="PLF67_00060"
     CDS             13610..15538
                     /gene="ftsH"
                     /locus_tag="PLF67_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOE88435.1"
                     /translation="MSNEHKNDKDWKESLKKRMNIEPGIHTGDKGPQKKYTFSFWYFF
                     IILMAFLLVNSFMVSRQPNIVQIDYNQFKQLVENRTIKRVNIETERYVGYSFSLGQLA
                     TDGIIPDGGAQAFATYKVEDPSFVPLLDEMGVEYFATPPPKPSIFSSILSYLLPFFFI
                     VILWRIIFSRMGSGGQGVLSFTQNKAKIVAEGDTGIRFADVAGVDESKYELEEVVDFL
                     KNPDKYTEIGGKIPKGVLLVGPPGTGKTLLAKAVAGEAQVPFFRMSGADFVEMFVGVG
                     AARVRDLFRQARENSPCIIFIDEIDAIGRARVGAGIGGNDEREQTLNQLLVEMDGFDS
                     RTGVIILAATNRPEILDQALLRPGRFDRQVLVDKPDLKGRYEILKIHTRKITLGDDVD
                     LNKIAQASAGLAGADLANIANEAALMAVRGGRKAVSQADFEEAIEKSVAGLERKSRIL
                     NPKERERVAYHETGHALTAYLTEGAEPVSKISIIPRGFGALGYTLQYPTEDRFLLSES
                     ELMINIDTLLGGRAAEEVVFGDISTGAGNDISRASDLVRRMITEFGMSDRYRNITLPT
                     TQSGIAGVGGSRDYSEKTQEYIDNETARIVNERYEIVKANLEKNKEALLTITSELLED
                     EVLSGDRFEELAKSKVIG"
     gene            15549..16484
                     /locus_tag="PLF67_00065"
     CDS             15549..16484
                     /locus_tag="PLF67_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOE88436.1"
                     /translation="MLYTPITDPELTRHLATLPKDGREVFVALDATVRITAISATTMI
                     NQMQANHGLQPLETYVLGQAYLATALLTSLVEGNDRMQLSIECGGPIGGIYTEGWANG
                     SVRGYLKNNPIPLTRELTSLDLDELYGPGFLTISKLLEGKKTPFSGQTMLQWGDLAKD
                     LATHFDQSEQTPTHFILSIDFNDSAQVIGAGALFIQAMPGWKQDGLDRLANCADELPS
                     LGKHIANGYSVKSFVETHFTGVDLRHLGSTDIRFFCPCTREQYGRYIQHLKESDRADI
                     AENGPFPFEVVCANCNTTYRWSKDEMKRLLDEVKR"
     gene            16481..18709
                     /gene="rlmKL"
                     /locus_tag="PLF67_00070"
     CDS             16481..18709
                     /gene="rlmKL"
                     /locus_tag="PLF67_00070"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HOE88437.1"
                     /translation="MIFYATSGLHQSDILAEEARMAGAEEIATSSSGVHFTGDLRVAY
                     RFLLTSHTATRLLLGIYEDDDIRSADEFYDASMMIPWEEWIDPSKSFSVTITTKNVRY
                     LKHSNFGALRLKDAIVDRIREHFDGRRPNVDRDDGDLIFHVHLEEDRATWFVDLSGQG
                     HHKRGYRGDQTDALLAEHLAFSLLYRSQWRKLADETGSFPTLLDPFCGSGTIVIEAAL
                     WATGRAPGLVKARIFPVFNLPIHDVDLYEEIVDEVVSRMEEESLSIHGWDRSPKAIRI
                     AEEAAREAGVDHLITFEVKDFTTLTAEDIPPSTGVIVTDPPYGMRMGEWEETRHLYRS
                     IGDALAQFFGGWNVSLLTADDRLLGQIDLKPNRTNAFNNGGIPCTLAHYYIFTHEEKA
                     ALRAEWEQRRKERLSQPLSSGAQMAYNRLIKNLKELKPVMEQEGVTSYRIYDADMIEY
                     NAAIDLYEGKWVNLAEYAAPPTVDVEDAERRLNELIDATERATGVDRESIFVKQRTVQ
                     KGLGQYERQAYTDRFYIINENNARYFVNFTDYLDTGIFLDHRPIRKYISEITDHKRFL
                     NLFCYTGTATVQAAKGGALSTVSVDTSTTYLDWARRNMELNGFGGMNHFYYQSDSISF
                     LWDTFDRYDVIFCDPPTYSNSKDRAPFEVQKDHRSLIKACMMHLDPGGLLIFSCNYRS
                     FRMDEEVYDEFDVEEITPQTIGKDFSRDPKIHHAFLIRERVKIVVQPQKPKRVIRRKP
                     VD"
     gene            complement(18754..19758)
                     /locus_tag="PLF67_00075"
     CDS             complement(18754..19758)
                     /locus_tag="PLF67_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88438.1"
                     /translation="MKLKFTALLAILILLPAMLFAVPVVITWEWLLEDPEVTTFRFQI
                     DSEKPEGWITVDSSVTSYTERGLDGTVDHTLYLQQSYDGIHFSESTRSVAEAMILAPE
                     PQAVVEEPVAPKPVTPAPSAPQPAAPVQEVKKAEPVATPPKAPKVKEPSRFYTKLGIN
                     LGYNRQRSRFNSAYDINNLKGGMTLEFHNILTFAKVFGLGVDLDLEYTPYLRGTTYRA
                     TLSKVLKGNFDALSTFFTSLEHSFTASPAVMLNVEGGRIALGLGGGGFLTYSLDPSFI
                     DSSTLTYRYGVFAKAALSYRLTDHFTLGIGGKYGVDLDKFASGGHQFLEATMSFGYIF
                     "
     gene            19894..20478
                     /locus_tag="PLF67_00080"
     CDS             19894..20478
                     /locus_tag="PLF67_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOE88439.1"
                     /translation="MRDSIQTLAVFCGSSSGHNPLYTEAAKAIAETMADNNIALVYGG
                     GNRGLMGIVAETVHRRGGYVIGVIPQLLNRADVVMRKVENELIVAHDMHERKALMYER
                     SDGFIALPGGIGTLEELLEVYTWLQLGYHKKPVAVLNIGGFFDHLLDLLTHANGEGFI
                     KDDIMNSLIVADDIPSLFEQLDRWDAQLGDKLSP"
     gene            20475..21395
                     /locus_tag="PLF67_00085"
     CDS             20475..21395
                     /locus_tag="PLF67_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOE88440.1"
                     /translation="MKINRYLIIEYFNVIAGSYLTALGIALFSAPSKIVNGGVSGIAI
                     IVHHSLGWDTGFSIFLFSIPLFLLGLLVFGKRYAVKSLAGTILLSVFTTMNNMIFGYE
                     GVLDPSNVLSVLLNSLSAGALMGLGLGMVVRSGSNTGGTDILAQVVARYTPLSMGTSF
                     LIVDGLIILVGALIFGLEMALYALITVFITSAAIDKVVLAFGRRSAKTVFIISTQLEA
                     IKDAILTELGHGGTILSGTGMYTRTDRPVIMTVVANTKLAALTHIVHRYDKNAFMVVQ
                     EAFNVLGEGFTPIEEGAWSSHLDITQEQRP"
     gene            complement(21392..22300)
                     /locus_tag="PLF67_00090"
     CDS             complement(21392..22300)
                     /locus_tag="PLF67_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88441.1"
                     /translation="MKPTLLILAAGMGSRYGGIKQIDSVGAYGQTLLDYGIFDAKEAG
                     FEKVVFVVRKSIEKEFRYHLFDRIARNMDATYVFQHQDSLLNEEQLELSVGRVKPWGT
                     IHAVLCAREEIHGSFAVINADDYYGKTPYVTLYNHLAGLAPDAKEHAMVGYVLENTTS
                     RIGTVSRAICSEKGGYLKSMVEHTKIGYEGDSLMSFRESGNLPLTGKEWVSMNFFGFA
                     HSAFDSFERYWHTFIEEHVTSEKNECYLPNGVGNLVAKGEGKVRFYTTDEHWFGMTYA
                     EDRENVKLQLAKKTEEGLYPEQLWLN"
     gene            22421..22699
                     /locus_tag="PLF67_00095"
     CDS             22421..22699
                     /locus_tag="PLF67_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88442.1"
                     /translation="MNNHERFTNVLEFCALSPTCRTCPYSLYTRRHQDASNKACFTSK
                     ENLEALERWLHSEHEEDDPAEEFLSDDQKWLQDLLKLQFNDDEDEFDF"
     gene            complement(22696..23259)
                     /locus_tag="PLF67_00100"
     CDS             complement(22696..23259)
                     /locus_tag="PLF67_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88443.1"
                     /translation="MQQLKRLVWAIIFTVIVLGSLSGNNSPVISSFTLQGRMPEDQYF
                     RIEVSTEAENVDLIEARGSIVPIGRYRLFSNVGNARFKIEIRPGEDGSGTRFKLYLTP
                     GTPHAPHLETEIPFTAGFIPYGDARAVAVEGSSIVSRSVGPQDRDSNSFGVQESGEIY
                     AKIDNFDPNRFATGTYSAAIQFTVTTD"
     gene            23621..23694
                     /locus_tag="PLF67_00105"
     tRNA            23621..23694
                     /locus_tag="PLF67_00105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23655..23657,aa:Arg,seq:ccg)
     gene            23772..24566
                     /locus_tag="PLF67_00110"
     CDS             23772..24566
                     /locus_tag="PLF67_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOE88444.1"
                     /translation="MSIRVLFLGEIVGKAGVQAVRQNLRTLREEQAIDLVIANGEGAT
                     GGFGLGKAHSMQLLKSGIDVLTGGEKIYYKLDMVEFIDKNPAILRPANFPQQNPGRGV
                     RYLNVGETKICVINILGNADFPRTHLTNAFPLAEYLVTKALEEGAIPLVQFHASPTAE
                     KQAMGFHLAGKAAAVIGTHSKVLTSDEQILDGGTAFISDNGRCGSQMSVGGFEPEAEI
                     EKQITQLPVRSKEYWEDTALVGVIVEIDETGKATAIEPIRSALKEE"
     gene            24569..24979
                     /locus_tag="PLF67_00115"
     CDS             24569..24979
                     /locus_tag="PLF67_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOE88445.1"
                     /translation="MLEVVTVQDIRSKDRIRVSCSSAACQGCKGGLFCNTKKRVFDVS
                     NIDGLAIEKGDRVEIHLKTGRTIGGTLLTLIAPLLLFPLGYYTAKSLGAGEGICTLIA
                     LGSIALGFVGVWIYFKVNEHSYLPTVKRVIKDAE"
     gene            25017..25556
                     /gene="lptC"
                     /locus_tag="PLF67_00120"
     CDS             25017..25556
                     /gene="lptC"
                     /locus_tag="PLF67_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOE88446.1"
                     /translation="MMKRILGMVLASLLLLSCALEPTIQSPVDSSKREADLTLYDATY
                     TFGMSNGTPLAVTAGVLAFHEEEQEAVLEHVTFIGYEEDGKPFFQGSARHAVINTTTY
                     DATLSEKVSLQHLDEEIHVFSDRLSWSDKNQQLSSPGDIASTIIIDGDITLRGEGLMI
                     DFTTLSVTFKKVLEGRVSR"
     gene            25553..26176
                     /locus_tag="PLF67_00125"
     CDS             25553..26176
                     /locus_tag="PLF67_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88447.1"
                     /translation="MKRLFFLILLIGFFLASASGNDITFGGGNTSIQTQAGRQVITLS
                     QGAHIQSGSIILKADTITLYGDGFRYVSCQGGLSLVDEERNLKLKANRLFYDREAEEI
                     LIEGYVELDDPANELIARGFGLEYRIDEQRISLHAEVTLEKHTSSGAMVCKADVMSFD
                     RTAMSLLLSGKASVHWAGDRYEAQQIVIDLEREEITAEGSIRGVIHG"
     gene            26169..26903
                     /gene="lptB"
                     /locus_tag="PLF67_00130"
     CDS             26169..26903
                     /gene="lptB"
                     /locus_tag="PLF67_00130"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOE88448.1"
                     /translation="MVNSLIVSNLSKRYGRRLVVDDLSFSMRSGEIVGLLGPNGAGKT
                     TTFYMIVGFLKVRKGSIILNGEDVTGLPMHERSKKGLSYLPQEPSIFRKLSVRDNILL
                     VAQSRNDLTRHEKHHLVDQLVEEFGLTHVVRQKGYTLSGGERRRCEIARALAASPTFL
                     LLDEPFAGIDPKAVYEIKLLIKKLASQRIGILLTDHNVRDTLAVTTNAHIINDGSILV
                     SGTKNELLAHELARKIYFGDQFEGEL"
     gene            26903..28258
                     /gene="rpoN"
                     /locus_tag="PLF67_00135"
     CDS             26903..28258
                     /gene="rpoN"
                     /locus_tag="PLF67_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOE88449.1"
                     /translation="MALTPSLTVTQKQQLKLTPQLIQSFELMTLPLADLQQRIKEEIA
                     TNPALEIPSTRELSYETVADRKVESELRRVDDSYGDTSTYGFDDDATERVHRVLEQVL
                     TAEETLAEHLIDQLRLERLTEEELEIGLRIITNLDSNGFFIYEPELFIDEAERGSYEK
                     VLSLLHTFDPVGIAVPSWRESLIVQAAYQGLEGETLQQFTALVMDNLELMRANKLEQV
                     AKNLGISEEELGDLYAFLKTLTPFPGQGFASGPQHYVIPDLSIRVEDGALQLRMNEDA
                     LPDIRINPEFEALASDPDAKTYIQEQVKKANDIIFQLDIRNKTLYKVALVLLREQREF
                     FILGPQYIKPLTQKAVAAEIGVHETTVSRIATAKWIETDFGIIPLKRLFSSAIGDLSK
                     EAVKETIRQILEEQEGQKALSDQKIADLLAERGIKIARRTVAKYRNELQIDPSYIRGT
                     D"
     gene            complement(28308..28607)
                     /locus_tag="PLF67_00140"
     CDS             complement(28308..28607)
                     /locus_tag="PLF67_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOE88450.1"
                     /translation="MNLTVRGIRYNPSDETREFLDKKLQKLQFAEGFLHDLDIVMTRE
                     TAGQGFHLDAKIHFSWGTVKMVSTDCYELYEGIELLVDKIEATARKEKSKVKNHG"
     gene            28758..29738
                     /gene="hprK"
                     /locus_tag="PLF67_00145"
     CDS             28758..29738
                     /gene="hprK"
                     /locus_tag="PLF67_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOE88451.1"
                     /translation="MAEFTVFDLLDLDIKERSHLRLRCVAGRSGLSRAITTSKISRPG
                     LPLSGFFEEFSAQAVQIFGRGEQAYIRMLEETDQMGNVEQFFSYPIPACIFTDHGEPS
                     KQIIELAESSSVPLLVSDLPSGDFSRILYQSLDEIFSPTQTIHGVLVEVYGIGVLIIG
                     DSGVGKSETALELMERGHRIISDDTVRLRNVSDTFLIGTGENPALAHHMEIRGLGIIN
                     LANLYGVGAIRDRKQIQLVIQLEDWDATKEYDRVGTEMTTSYLGISIPLVILPVKPGR
                     NIPIIIETAARNERLKKLGYFSAKEFDQNVKKWLESESARKIYYDAEEDF"
     gene            29738..30001
                     /locus_tag="PLF67_00150"
     CDS             29738..30001
                     /locus_tag="PLF67_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOE88452.1"
                     /translation="MVQRELVVTTRAGIHTRPAATIVNVANRFESDLFFERGTIKING
                     KSIMGIITLGATYQTSLTMICEGKDEEELADAIEALFLNRFEE"
     gene            30006..30629
                     /gene="lexA"
                     /locus_tag="PLF67_00155"
     CDS             30006..30629
                     /gene="lexA"
                     /locus_tag="PLF67_00155"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOE88453.1"
                     /translation="MIRPLTNRQRDVATFITDFIKQNSYPPSVREVAEYFQFSVKAAH
                     DHLKALEAKKVIRTTPGSSRSIEIIDEEFLETEEVIEIPVIGTIAAGKPLLSEENTDY
                     MLKVPVSMLKGARATYFALKVRGESMIEEGIFDGDLAIIRKTQSANSGAIVAATVEDG
                     EEGITLKGYYPGEDMIELRPANSAMGPIFTRSCRVHGILQLLIRDFS"
     gene            30626..31654
                     /gene="holA"
                     /locus_tag="PLF67_00160"
     CDS             30626..31654
                     /gene="holA"
                     /locus_tag="PLF67_00160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOE88454.1"
                     /translation="MSSYLLLGPEAGAKADEVKRIRSELAAKYGDGLEFERFYPFDGE
                     QGELFASLANDSLFSDHRLILISQLESAGAAFLNELAGYLANPSPSATVVLASSETSI
                     TKKIMDAIPKERTRIFYDLLEGEKSAWIRTHFRRLGLTITSEAVELLIAMTEDNTQEL
                     RTVCNQLALFWQHDSPTKPIDEEAVQTYLHHSRVEDAFTLFPHLAAGDLKKALAGLHA
                     LLGSGDYQTPILLVSGLLWQFRRLLSVAEGFEEGKDEPEVFSNAHVLLKNSAIRRPKD
                     KTTYREGAKRYSSKDIRSIIIALAQADIEVKQSRSEEIPLILERLIYRIINKKGKELT
                     TASFATLL"
     gene            complement(31649..33493)
                     /gene="uvrC"
                     /locus_tag="PLF67_00165"
     CDS             complement(31649..33493)
                     /gene="uvrC"
                     /locus_tag="PLF67_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOE88455.1"
                     /translation="MAKKETRYTVGSEIETFNTVVSEPIAPSLQARELPGQSGVYLMK
                     DKDDRIIYVGKAKDLRKRVTSYFLANRSAKTAALVRKIVTIEYIITGNEYEALVLENN
                     LIKKYAPHYNISLKDGKSYPVIRITNEPFPKVLKTRQIINDGSTYYGPYPGARMLEQY
                     LDLIYKLFPLRYCSTPLRKREKPCLYYHIGLCSGPCAGLIGAEEYGRYITEVRNLLEG
                     KVDGLLTQLHEEMTKASRELRFEEAATKRDQAETVKMISTDQQVQEFSEENRDYAAIE
                     MRGPLCTISLMQMREGQVIGRALYRAETFGDETETLLAFLIQYYADGMKLPQYLYVSH
                     EIEVDLIRRYFLEHFGGILEVSLPIEGKHYRILRMARENATRDVEKRLRNKDNTEALH
                     LLKELLGLESEPVLIEGFDVSHLQGKYTVASLISFRNGNPDKPNYRRYNIKSVEGRID
                     DYDSIREAVARRYQKVLNENLERPGLLLIDGGHGQVNAAREVLDDLGIFDIPVIGLIE
                     GSETIVFGDGKEDLRLDHGNEALRLLIAVRDECHRFAHASNQAARSRTASFQLLQSVD
                     GIGKKRSEALMAQYGSLEAVLKKDPEDLAKEAKLPLAVAQRLIKQLSL"
     gene            complement(33575..34396)
                     /gene="thyX"
                     /locus_tag="PLF67_00170"
     CDS             complement(33575..34396)
                     /gene="thyX"
                     /locus_tag="PLF67_00170"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOE88456.1"
                     /translation="MAHNVVEAAEAILDKEFKVLDHGFIRLVDYLGSDERIVQSARVS
                     YGDGTKTYRQDKGLINYLLRNDHTSPFEQVNFTFHIKMPIFVARQWIRHRTARVNEIS
                     GRYSVMKDEFYVPDSRHINLQSEDNKQGRMDEAVDPALAKKVQEILAADQKRSYETYR
                     QLLEMGLARELARINLPLSMYTEWYWQMDLHNLFHFLKLRLDPHAQWEIRVYAEQILK
                     IIRLVCPIAVEAFEQHKVGSVTFSASELEALKDVLDGKENPLTGRALQLFEEKLK"
     gene            complement(34420..35130)
                     /locus_tag="PLF67_00175"
     CDS             complement(34420..35130)
                     /locus_tag="PLF67_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOE88457.1"
                     /translation="MNIVLFEALEPRNTLDLNDYRAKHIKKILKLKEGDSFKAGVINT
                     STGVSTITSVGEKSIEFTYTEEKPAEPLYPLTLLVGMVRPISMRRILREAVSLGVKRL
                     ILVPTDTGEKSYVQAGLYTSGEYRSILLDGAMQSGHCQLPEVVFTASLDEEYPYEGVK
                     IALDNVEGSISLDRVEMNPEQGATLAVGPERGFSDRERILLGERGFILASFGTRILRT
                     ETICSAAVGVLLSRLGFL"
     gene            35177..36133
                     /locus_tag="PLF67_00180"
     CDS             35177..36133
                     /locus_tag="PLF67_00180"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOE88458.1"
                     /translation="MNLAIYYSTSHDCAANLAGEAYLMDLPYSRILYLWVNDPCVVIG
                     RFQNPHSECNLEAMKKDGVQLVRRQSGGGAVYHDRGNLCFTLIGDKETATKEENFVLV
                     IDALAELGITAEQSGRNDILFDGRKISGNAFQTTKTKFVHHGTLLVKSDLSVMANYLT
                     PSDTKLASKAVKSVASRVGNLKEAKDDITVKEVESALEHTFKERYGESEVVHIDFSEF
                     EPASANYRLFGDLDLILKRTPPFTHSFTHRFAWGEVTLNLHVIEDTIKMVTLFTDSLD
                     TTLRERVEALLVGVRYEAAEIAGLNAGEPALNELIAYLLARL"
     gene            complement(36130..37644)
                     /gene="malQ"
                     /locus_tag="PLF67_00185"
     CDS             complement(36130..37644)
                     /gene="malQ"
                     /locus_tag="PLF67_00185"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOE88459.1"
                     /translation="MSTTLRKTGVLLHPTSLPSRYGIGSLGKEALQCIDLLDKTGIRL
                     WQILPLGPTGYGDSPYAARSSFAGNELLIDLEELMVDGYLEYEDIYTVPVFDKDRIDY
                     QAVRAYKEPLLAQAAGSFLKHANKSERSEYKRFCKDHASWLEDYALHNVLCAVYNDSR
                     WFEVWPKELGLREPEALAAARKQYSAEIEEVKVLQFFFYRQWLKVRSYANERDIEIIG
                     DIPIFVAPDSADAWANRHLFKFDEDGKQLASSGVPPDFFSETGQMWGNPVYDWPVHVK
                     EEFAWWTERFRHTFLQCDIIRVDHFRGFAAYWEIPYGNPTAEIGSWEPSPGVELFKTL
                     KKNLGDDLKIIAEDLGVITPDVEELRDSNGFPGMKIFQFAFTVEKGVFDPTNEFLPYN
                     CSENSVIYTGTHDNNTTLGWFRALTGEEQDVVRRYFEAPDAQIVWQMIRQMLHSVCRW
                     AILPMQDWLELDASGRMNTPATVGSANWSWRLSSLKLEPWRIERLASLIRLTGR"
     gene            37732..38235
                     /locus_tag="PLF67_00190"
     CDS             37732..38235
                     /locus_tag="PLF67_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOE88460.1"
                     /translation="MSLSEKTLPFSVGEHVVYPLQGVGVITKIEEIDFRGVDTLYFVI
                     YLDISDMTVRIPVDKYQDMGIRPIVGKEESQAAIDSISSKYEPMPVDWKARYQMNVDL
                     LKEGSIASIAKVVQALYHRSKIKELPVQERKLYDTALRLLIDECSISLEKDEKEIEMV
                     VFSRLES"
     gene            38239..39048
                     /locus_tag="PLF67_00195"
     CDS             38239..39048
                     /locus_tag="PLF67_00195"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HOE88461.1"
                     /translation="MAFPSHAVIVTAAGSSDRFNESKEIGVKKEYLNLGGHTVLYQSV
                     RPFLGIPNCRAIVVTHPEGMEDQCAVALEDLLEQNLVPIILVKGGKNRQESVLNGLEL
                     LTALSLPIEYVAIHDGARPYVSEELIITTLATATIFGGAVPALSTTDAIKTIDEHGQI
                     IGHSNRQQTVGVQTPQIFRYPEILEAHRRAAGTTNAYIDDTEIFTDYNQWVGICEGER
                     TNIKITYLTDIPDAEEQIKRYLADLEAGKKQARINEAFGCALSQAKPRRMR"
     gene            39045..39521
                     /gene="ispF"
                     /locus_tag="PLF67_00200"
     CDS             39045..39521
                     /gene="ispF"
                     /locus_tag="PLF67_00200"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOE88462.1"
                     /translation="MRIGSGWDIHRLEEGATLVLGGVIIPSAKGLVAHSDGDVLIHAI
                     IDAILGALAEGDIGTHFPDTDPRFEGASSVDLLDHVITKLLPPYSIGNIDTTIILKEP
                     KLRPHIDSIRYSLADILEVPIDHVSVKAKTAEGLLGELGTGDAIEAHATVLLTIKG"
     gene            39543..39626
                     /locus_tag="PLF67_00205"
     tRNA            39543..39626
                     /locus_tag="PLF67_00205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39577..39579,aa:Leu,seq:cag)
     gene            39731..41116
                     /locus_tag="PLF67_00210"
     CDS             39731..41116
                     /locus_tag="PLF67_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOE88463.1"
                     /translation="MEEGLTLEFKKELAEGLEKEVVAFLNTQGGNILIGADDGTILGL
                     DDAKSLSLIVIDRIKNNIQPATLGLFNVEVKQAESKSYIRVIVAQGLEKPYYIKKHGM
                     SSKGCFIRIGSQSSPMNQSMITDLYSRRAIQTLSNVLSPNQHLTFDQLKMYYGIRGFD
                     VSGEFFLRNLGLYTDDGRFNYTAYLMSDDNGVSIKVARFRGMNKLDIIERNEFGRTCL
                     IRSAYQVLDKLEVANATVVRVGGKAARKEIRLVDRDALREAVLNAIIHNDYILGSFPV
                     FEIYDDRIEIVSSGGLPIGLSQEEFFKGLSHPRNRELMRIFSDMDLCEQLGSGMQKIL
                     RAYPRDIFSISDHFISVRFPYNEEALSIYREEDPCKPSGGNSGGDGGNNGGNTITMLP
                     AEDDGLSENALTVLRALQTDGSLSQRQLSESIDMSMRTVQRALTELRDARYIVREGSP
                     RSGTYIVLKQV"
     gene            complement(41244..41489)
                     /locus_tag="PLF67_00215"
     CDS             complement(41244..41489)
                     /locus_tag="PLF67_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOE88464.1"
                     /translation="MMIINTKQIVSMTEANQNFSKVARLADAQGNAVIFKNNRPKYLV
                     IDLAQETFLNLTDAEKIEIVARRILEKHRKAFEALAK"
     gene            complement(41701..42498)
                     /locus_tag="PLF67_00220"
     CDS             complement(41701..42498)
                     /locus_tag="PLF67_00220"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE88465.1"
                     /translation="MKVALITGGSKGIGREAARLCLKEGMIVYILGRDVEALEETANL
                     LDPEQKVLFSIQADVTDHLACKRAIDEVITRHGRLDLLINNAGMSMRGRVNETTPEVL
                     EAMVHINFLGSAYMTYYALPHITGQKGSIIFTSSLVALHGVPKVAGYAAGKGALTAYT
                     ESLRAEVSSKGVHIGIIYVGFTENDPKKVIYSADGSLMPLSKRKNAHTQQDVARYMLK
                     MIKRRKKRMVLTPLGHFSSFLYRFFPNLSDWIIARFNPTNAQYSEAN"
     gene            42625..43206
                     /locus_tag="PLF67_00225"
     CDS             42625..43206
                     /locus_tag="PLF67_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOE88466.1"
                     /translation="MLKPYDLVMYDLRSYASPKAKLTQMIRKGEIIRIARGVYADSFD
                     DPRLPVAGMLVSPSYVSFETALGYYGCIPERVHAIKSAGFNLNKEKRFDTPFGLYTFH
                     YIPRNVFPLALTPAEEQGHGFRLAQKEKALLDTLYKIRGITTKKGISDLLFEDLRINE
                     LVFEELDWELLSALAPYYRSTTINTLIRHGRNI"
     gene            43203..44075
                     /locus_tag="PLF67_00230"
     CDS             43203..44075
                     /locus_tag="PLF67_00230"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE88467.1"
                     /translation="MRNEVTAMVNLQRSNSPIEAVNALKEAIQELVLKALSKTDFFNR
                     AAFYGGTALRIFHGLPRFSEDMDFSLVKPDPTFRLSAYFRSIEEELGSYGFELKFISK
                     EKRTVSPIQSAFLKGNTEYHLIKFLELQRPISGVARNSIITIKLEVDVAPPDGATFER
                     RFRLLPSPYSALLYDAPSLFAGKLHALLCRKWATREKGRDFYDYVWYLQHKIPVNLPH
                     LEERMRQSGDWMNNDKLTKADLMHLLHDRFLRTDYHLVTQDILPFIKDSSSLDLYGTD
                     FFNSITDEYLQIVQ"
     gene            44419..45579
                     /locus_tag="PLF67_00235"
     CDS             44419..45579
                     /locus_tag="PLF67_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE88468.1"
                     /translation="MGGLFAQGAKDEGAVANKDKPLVWFNRQPSDPVTGKIDMDSMNW
                     NSKTYYVGFDAAGGGAVQGKLITDYLAAANPAKLDRNGDGTIGYVLCIGDVGHNDSKA
                     RTEGIRKALGTWNGSTDPGAAKVGSVVVGGKTMRVVELEGKAMTGTDGSTWNANAATE
                     AMGGWATKFGNQIDMVVSNNDGMAMGSLQASNYPAGVPIFGYDANADAIEAIGAGRLT
                     GTVSQNVDAQAAGTLQVLRNLLDGLTGADVYKKGITEADQWGNKITAPLDYWADVKAV
                     MAANSGVDSTNWKQYTEGSRDAGIKQSAAPKKRVLLTIYNASDNFLSSSYVPALQYYA
                     PLIGIDLTIVQGDGQNESSTLDKFTNLGSFDGYAINMVKTNSGPDYLDKLRY"
     gene            45655..47148
                     /locus_tag="PLF67_00240"
     CDS             45655..47148
                     /locus_tag="PLF67_00240"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE88469.1"
                     /translation="MQEPVLEINYLSKSFAKNKVLDNVAVTVHKGRVLGLMGENGAGK
                     STLVKCLFGIYAKDEGQMFLNGKPIDFKNPKEALENGVAMVHQELNQCLDRSITDNLF
                     LGRYPLRFGVIDEARMLMEANQLFASLNMNINPRTIMRTMSVSHRQMVEIAKAVSYDA
                     KIIVLDEPTSSLTEREVKKLFSIIDDLRKKGVSFIYISHKMDEVFEICDDVEVLRDGK
                     MVLSKPVAETTMDEIVSALVGRSLDKRYPDVDNNPGEEYFHIRNLTTKFDPVLQDISF
                     SVRKGEIFGLYGLVGAGRSELLEALFGIRTIASGEIVLGNKKLRFKNAKEAMAYDFAL
                     ITEERKLNGMFGKDTIKFNTVITNLESYKTMNVLSNRKMLDAANRQIVQMRTKCVSAE
                     ELISSLSGGNQQKVIIGKWLERQPEIFLMDEPTRGIDVGAKYEIYQLIIRMAKEGKTI
                     IVVSSEMPEILGITNRIAVMSNRRLAGIVNTNETNQEELIRLSAKYL"
     gene            47162..48844
                     /locus_tag="PLF67_00245"
     CDS             47162..48844
                     /locus_tag="PLF67_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside ABC transporter permease"
                     /protein_id="HOE88470.1"
                     /translation="MNADYQEIMELTEDPKIIEYSQTLNKLRENGVNKIKKLRQDIVA
                     LRKSKMVHPQEKRKQIKAWKEEIHLAKKDAAQNKAAIDELVKESVAYANKAAKTFIEQ
                     VTIREDEAIAKAKQAYLEEVRTIKEEAKRSETAIRSEYKGRSRKELKAELEAHRYKTK
                     TALFDARSHRQQAIDQALAAKNQAFVDHVQTNRNLRNGKTKFSEDRQLKRREYRYNFK
                     LSQFLLANGLYIAIGIFFIVVIILAPLSGAGNLLTLPNILTILEQASTRMFFALGVAG
                     LILLAGTDLSVGRMVALGAVTTGLILHPGKNIVSVFRYPTWDFTPMAMSNRVLMALGL
                     SILLCVAFSSFAGVFTARLKIHPFISTLATQLIIYGLLFFGTSGTPVGSIDRNIKDAI
                     GGRWILGQIGSQYVTFPKLIIPALFAIFIAWFIWNKTIFGKNMYAVGGNAEAASVSGI
                     SVFKVTMGVFIMAGIFYGSGAFLEAFRANASAGTGQGYELDAIAACVVGGISFNGGIG
                     KISGAVVGVIIFTGLTYCLTFLGIDTNLQFVFKGFIIITAVALDSVKYLKKK"
     gene            complement(48936..51269)
                     /gene="yicI"
                     /locus_tag="PLF67_00250"
     CDS             complement(48936..51269)
                     /gene="yicI"
                     /locus_tag="PLF67_00250"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HOE88471.1"
                     /translation="MKFRDGYWHIKNNTQYMNRVDIVDMQQDEGKLTIFSAVKKMLHR
                     GSTLNTPLLTTEITSPMPDVLHVKSYHFKGFLNKGPFFDVVKDVPSHLIIQKNDTKAV
                     ATSGSLSIMINAEKNCDYTFRYKEKELTRSLGHLSGHAVVDHNNPFQVEYLSLGVGES
                     LYGLGERFTPFVKNGQVVDIWNEDGGTSSEQAYKNIPFYLSSKGYGVLVADAGRVSFE
                     LASEVVTAAQFSVSGQCLEYYVIGGKSLKQVLSNYTKLTGTPALVPPWSFGLWLSTSF
                     VTNYNEETVHSFIDGMEQRKIPLHVFHFDCFWMKEFQWVDLLWNPVTFPNPKALIAGL
                     HDRGLKVCVWINPYIAQKSSLFDEGMEKGYLVKNPDGSVWQWDRWQAGMGLVDFTNPQ
                     AVTWYQSKLKALLDIGVDSFKTDFGERIPTNVVYHDESDPHLMHNYYTYLYNEAVFSL
                     LEHERGVGEAIVFARSATVGGQKFPVHWGGDCSATYESMAESLRGGLSLSLCGFGYWS
                     HDIGGFEQTATPDLFKRWISFGLLSTHSRLHGNESYRVPWNFDEEASEVLGFFTRLKC
                     TLMPYLFSQAVVAHQEGIPMMRAMVLEFSEEPATLYLDRQYMLGESLLVAPVFSADGK
                     VEFYLPKGRWTHLLSNEEIEGGRYISQTYDYFSLPLFVRPNTVLAIGANETEVAYSYN
                     TDTTFHVFSLNENTPTGAKVFAHDGSLLASCTVTRCRSTLEVTAEGSLRKWAVCLRNI
                     YKIRSHSAGEVTQTPSGSVITFGSHEKTCLIELMEGT"
     gene            complement(51293..52123)
                     /locus_tag="PLF67_00255"
     CDS             complement(51293..52123)
                     /locus_tag="PLF67_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE88472.1"
                     /translation="MKERFILPYVWTIVLTLIATVYLYPLFLVLINSFKTFQEITSNV
                     IALPTSLEWHNFANTFKVMNYPRYFLNTLAATSIGVLGVVSISSMAGYKLSRTNSRIS
                     FIMFMVLIAPMMIPFHSFMISLVKVAKELKLIGSPAGLGVLYWGLGASLALFMYHGAV
                     KSVPRELDDCASIDGASPLRTFFQIIFPLLQPVTVSVIVINTMWMWNDFLLPLLVLSG
                     SKKSLTLQLAAYNFFGLYKIDWNYAMAGVLLTILPAIIFYLSLQRYIIKGMVAGAVKS
                     "
     gene            complement(52120..53010)
                     /locus_tag="PLF67_00260"
     CDS             complement(52120..53010)
                     /locus_tag="PLF67_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE88473.1"
                     /translation="MTQLQRKTFNRTLVFLLFTAPAFLMVLISVEIPFLMSVFSSFTK
                     WNGLDKAQTFIGFQNYHELFTDDPKMWGAFGFTLKLTLGSVVLVNILALLLAVILDSD
                     VKGKNALRAAFYVPNIISLIIIGYIWRFIFSAGFNSLYEKTGLEIFMLSWLGDVKVVY
                     FSVLMVSIWHSLGFYLVIYIAGLQTVPHELIEAAMIDGANKTKRFFNVTLPLIVPSIT
                     VSVFHSLSNGLKSFDIIFSLTNGGPGNVTTTIALDIYRTAFVINRFGYGTAKSVVLFL
                     MILVLSIFQVQIFKQREVEV"
     gene            complement(53066..54322)
                     /locus_tag="PLF67_00265"
     CDS             complement(53066..54322)
                     /locus_tag="PLF67_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE88474.1"
                     /translation="MKKVLIFSLVVFLAMTTMFAGGQADKKEKATTLDVLFYSPELAE
                     QYNDMVAAYKAETGVTLDITVLQTDYRSVLTSRLNSGDVPDIFMSSAYADNSTYKDYV
                     YDLTNEDFISLIEPGTLAGVTLDGKVLGYPFLVQSHSFIYNKKVFRDNGITDLPRTFA
                     EYEAVSKKLEANGVQPFATGFHEFWVLPQTAWQAIADVPVSKYGGYEQFVAMLNSGQL
                     KFKDIPEMSRIFDLLDLIKRYGGNKPSESDFNDQTAGLATGRVAMIHQGNWAEDTIRG
                     INPSVEIGFLVGPTGNNATTAGVMFDSNQTIRIAKDSKNLQAALDWLRWLTTSEYGKA
                     WIPSKVKQLSPIIGAAAPDSMVAEETTKLMAAGAPGYPWFYQMFPTGTEQELGAILQG
                     YCAGLTDRAQTLDALDSAYAKIAGAM"
     gene            54432..55526
                     /locus_tag="PLF67_00270"
     CDS             54432..55526
                     /locus_tag="PLF67_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE88475.1"
                     /translation="MGITQKEIAKHLGVSYMTVSRVLNNSNVYVSDHLRKKILDYAEE
                     MGYEPHRASQVLVRNKKRHIAIFSSTLPTYFWEEINKGVQIAATQIKPFDYTVRYHQV
                     PELDTEAYLALIKQELKQGVDAIGLVNQRMYDMDRIIALITEAQIPYIFFNVDSPTNN
                     RLCYVGSDYRDGGRLAANFIAVTLQLSPNKEVLVLQATESKLQSSRAPDINKLRLEGF
                     MELVTTKHSNISVHVEFFDSLQQDTETFTQIHEIIQRYHQKVSAIYLIPAFNTEFLRA
                     LKEIPKSNIITILHDLDSTASSHLKSGELSAVIDQSPTLQGYYTVMTLEKLLERGSEH
                     ILSFVKVDHNLVLNENRQLIHGLIEGQFLI"
     gene            complement(55580..57613)
                     /locus_tag="PLF67_00275"
     CDS             complement(55580..57613)
                     /locus_tag="PLF67_00275"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOE88476.1"
                     /translation="MTTIRKTVYRAQAALAAGSSPLPSFRDPDHDLPVRLKEDVPKRY
                     RRYLGLDCGRRALPYRMQDRYDRNRTATDIPAIVLENEFLAVTFLPSLGGRLISLFNK
                     AEKRELLYANKNFQVGNLAILDAWFAGGIEWNIGQYGHAFSTNLPVHASVQKDEEGRQ
                     FLRLYSFERAKELYFHLDFHLRGDLLCVHTAVHNLKETDASLYAWVNIAVETDEYLRV
                     LASNPKAIYLDSYVPNDERLFGIMEMPTMPIYQGIDVSYPNRFIASNEYFFTCEDDEL
                     PWECALGKDGQGLFEASTHPLSYRKMFCWGTHSGGKRWQRYLSPDSTIDYVEIQSGVA
                     PTQLHGEILPARSSVSWTQAFGLLKVDPGKAHELDYEVARRSVEEAVTRRINLASLKE
                     IDREVTRSASIRPGSILHRGEGWGYLETRFQTEELPPAFRFEAADVGAEERLYLEFLE
                     SGLLPSLGGSSTFVPPVSHRWKKRFEEALSKEMSDAGRASLLHVLGIIYLEEEQVSLA
                     ETMFLQSLDLHPSAYTARNLGQLELRRSELPEALVWYEKSTMLPGFEEDWAIASEYLS
                     LLLTTEAFETVRVVFDSIPPRWLEESEALRLARARLAVWEGNPELIQSLVFDRTLAHI
                     REGETPLAELWNEMCVLRYCKKHGIEEITDDERKKVLARNPIPPEYDFEMKLE"
     gene            complement(57624..58697)
                     /locus_tag="PLF67_00280"
     CDS             complement(57624..58697)
                     /locus_tag="PLF67_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOE88477.1"
                     /translation="MHQSHSDLADIIARFHIEGRLVALKQTNEGHINDTFFSTFDEQG
                     TVRRYTHQRINHFVFKNPPQVMENIHLVTSHIQAKLAQTYDDYSKRCLVIVPTKDEGL
                     FHRDEAGNFWRTYRYIDGVKTIPRVENVNQAYLLGKAVGTFQTQLADFDGSRLHQTIA
                     DFHDMRVRYDQLKEALTLDRFNRAKDVRPELEYLDQNRKRGYVLIDGFKSGQLPVRVT
                     HNDTKISNVLFSEDGLEALCIIDLDTVMPGTILFDSGDMIRTATTTAVEDEKDVSLVH
                     CNKAFHDALLEGYFSYAGFLTHEERELVVESGRNITQIMAVRFLTDYLGGDAYYHTEY
                     EEHNLIRARNQIALMRDMDRLFG"
     gene            complement(58719..59579)
                     /locus_tag="PLF67_00285"
     CDS             complement(58719..59579)
                     /locus_tag="PLF67_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE88478.1"
                     /translation="MARKSVTFEMRNQKRLIPSYIVIVTWVVFTFVLIGWVIFASLST
                     SQEIFSDHMFRFESGFHFENYARAWRTQKVSVYFMNSVLYTVISCTAIILIAAPAAYV
                     LSRFRFKGNLLIQNMIASALGIPAIMIVMPLFGLVSRLRLTNNRWLLIFLYVSMNVPF
                     ATFFLLSFFKNLSSTYEEAAAIDGCSPTGTFWRIMLPLVQPGIITVTVFNFITIWNEY
                     FMSMIFANKTTVRPVSVGLFNMIQAMRYTGDWGGMFASVVIVFAPTFILYIFLSERII
                     QGVTAGAIKG"
     gene            complement(59589..60497)
                     /locus_tag="PLF67_00290"
     CDS             complement(59589..60497)
                     /locus_tag="PLF67_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE88479.1"
                     /translation="MKRNRTMIITFLAPAVLCYLLVFLYPSVRTTIMSFFKVESVSDP
                     ISSWRFNGLGNYRTLFATAIFKQAMRNIMNIWLVGGLGVMAVALFFAVSLTNGMRFVR
                     FFRSVIYLPNVVSAIAMGTMWINYVYNSSYGLLHNIFAKLGFTALSETLWTGPGNLFW
                     SMLVAYSFGMVGYHMLIFMASIEQIPRDYIEAAFIEGANVFQRFFKITLPFLRGCFRT
                     NIVMWTVFTVGFFVWGQLFSPVNLSNATVAPMNYMYEIVFGSSASTTTVRDSGAGAGI
                     GVIMMVIVIIAFMLTNLIVRNDDVEL"
     gene            complement(60555..61808)
                     /locus_tag="PLF67_00295"
     CDS             complement(60555..61808)
                     /locus_tag="PLF67_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88480.1"
                     /translation="MKQKVFVILFVALLLVSPLFAGGGAEAKSDKKTVTYWSMWNEAE
                     PQGLVIAEAAAAFEKEKGIKIDVVFNGREIRKTLQPALDAGETIDIFDEDISRVNSTW
                     GKYLLPLEEYVSKSYPTTGGVAYKDAVATALLDLARAEGGGTLKCAPYQPFAFVVMYN
                     KDLFAKAGITAPPKTWAEFEDVCAKLVKAGITPITVDDAYMAAFFGYNLTRMIGYEKT
                     LAMADNNDFKDPAVREFGQIWSDFAKKGYISRQAASNIYPAGQIEEIAAGKVAMYLNG
                     TWLPNEIKGNAPNLNWGAFAYPAIKPTGVGTEANNYGAQCFAINVNSKVADEAFDFIV
                     YMTTGKWDAKLAEESLGVPMGKDSAWPKQTAEAKTVIDNTTKWMTWAAGMENLADVNA
                     KIKENFAKLILGQLDAEGFYQAMNR"
     gene            62017..62928
                     /locus_tag="PLF67_00300"
     CDS             62017..62928
                     /locus_tag="PLF67_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE88481.1"
                     /translation="MEPKINGFTPIEMKIDIEVSRFVTFHYFEYPHKFVFEGEKHDFW
                     ELLYVDKGVVFVGTDEDRHLLRQGQIIFHKPNEFHTVECDGIISPALVVVSFVSESEA
                     MKFFENRVLRITQRTKSLMSAIILEAKKSYSNDLGDPFFTKLLRREPKPFGSEQLIKN
                     YFEAFLIELILQGGREETLLGTTQAQDRRIRFEVANAFLEKHLGDNITLDDLCNHCSM
                     SKSLAQQIFKHETGSSVMQWYARLKIAKAKQYIREGKGNMTSIAYKLGFSSIHHFSRQ
                     FSKIEGKSPTEYAQSIISLTNKDRILL"
     gene            63013..63345
                     /locus_tag="PLF67_00305"
     CDS             63013..63345
                     /locus_tag="PLF67_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOE88482.1"
                     /translation="MQHTPHTLITVIVNQGMAEEVMEAARSVGATGGTIVPARGTAKE
                     EDVKFFGYPLVPEKDMLLILSGSDQTGRILEAINNLPILSKPGIGIVFCIDVERFMVF
                     GAPRNNLF"
     gene            63393..64568
                     /locus_tag="PLF67_00310"
     CDS             63393..64568
                     /locus_tag="PLF67_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE88483.1"
                     /translation="MGELSKRPIFVRLAAPTWGRWVLHRNRITGHGLEKMTTIDGPFL
                     VLCNHTHTLDPFFISALSPVHIRWVAGAYLFKLPFLRMLLHHLVGAISKQQGRSDMHM
                     MRQITAAFKKGDVVGIFPEGTRTWDGEPMSFDSSLAKLIKFFKVPVVILNFEGLYAIK
                     PRWTDHRRKGNVVLAFKSLLTVEKAQAMSVNQLYEHLKSELGFSYRRWQRRVKARFIG
                     KRSAEGVEKALYLCPSCANGSTITSKGRTIACTACNLSYTLNEHERLENNAGGKPFFE
                     DIAAWREWQRSYLITHHPSFPEDQGVLFQTGNETHLKTITTTFSLRLEEDNMTLTTAT
                     QTYRFDFDHVTSMIINAKNTLEFYCNGFLYRVRIAKHLSVLKYVERFQSYASTEATQ"
     gene            64565..65983
                     /locus_tag="PLF67_00315"
     CDS             64565..65983
                     /locus_tag="PLF67_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HOE88484.1"
                     /translation="MNWNFFFHVGIISFALLLSALIRARVRFFQRYLIPSPILAGMML
                     LIFYNFGAPKLGLRSDFLGDMVYHLLNISFIAMLLRVTPTAKVKDQARRTLAANVTAV
                     MAQYGLQAFFGLVVTALLIAVYDKNLFPAIGYTLPLGFELGPGQAYSIGMGWEPMGFK
                     GASSVGLTMAAIGFLVGSFGGVVLINQGIRRGWISKADAKRIDNKSVRTGFFSRLESE
                     RPIGAYLSTDGESLDSFTYHIAIVMLTYLISWGFLTGLSFLLGLIGPMGTELADSLWG
                     INFIFSAFCALAVKMFMKLVGIETTIDNATCNRINGLSVDLTVAASLGAISLVAVKGY
                     WVPIVALSITGIIITAFILPWYTSRLYDDHQFLRMLLLYGTATGTLPTGLALLRVVDQ
                     EFETPVATDYLYSVGIVFILAIPFILTINFPAFAVTKNNPVFFYLTILIAGVYLFGSF
                     VAYLLIAKKRSFIQPKKLFYVE"
     gene            66142..67008
                     /locus_tag="PLF67_00320"
     CDS             66142..67008
                     /locus_tag="PLF67_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE88485.1"
                     /translation="MRSKESITCKPYIMIFPALLLAVLFSYVPFILTFLNSVHAVSLG
                     GKRLGFVGLANYRRLFGNPSFHASLSNTLRFTLFFVPTNIIITLLAAVLSNRKGWLAN
                     LNQIFFFLPMAVGMSSMLMILKMMFNPTIGIINRLIGVNIQWFNDPNAAMALLVIAGV
                     FLDIGFDFLLFSAALRNVPNDLKEVALINGANGFQIFRHLELPLIAPTLIFIVVTNIK
                     NAMLISAPVLILTEGGPFRSTQTLVYQMYLEGFKSGNYALGSAIAIVVFLLTFAALLI
                     LLHLERRRIYYR"
     gene            67005..67811
                     /locus_tag="PLF67_00325"
     CDS             67005..67811
                     /locus_tag="PLF67_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE88486.1"
                     /translation="MKTLRATLSTLLSLLIVFPIVYSISASFFQYTDFTALPARLFPS
                     RLNIGNYVRAFSESFLARFLLNSLLTAIAGTLLRMAISILAAYTFSFFTFKGRNALFI
                     LIVATMLLPSDALIIANFMTIRALRLTDTYLGIIATSLLAPTHIFMLRQYFRMMSVEY
                     REAAIIEGCSDSRFLSMLLMPMSKAVILTLAIHSFSTIFNDYLWPLLVTNKTSMRTVQ
                     VGLTMMGFSEQLDYGPQFAAITFLMTPILIVFIALHKPIIESVSIRFTGR"
     gene            67815..69152
                     /locus_tag="PLF67_00330"
     CDS             67815..69152
                     /locus_tag="PLF67_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88487.1"
                     /translation="MKRFITIVVIALLALPMLFAQAQEESAPYTTITWWHPNSGLVGT
                     ASEELIKTFNRTVGEDLKIRVEGVYQGKANDVLTKLKPILQSKDQRDLPDLAQLDGSA
                     VLDIRDSAQLITINELAGNEPEYLSKIVEATLNSITYQGKILAMPFNASTILLYYNKD
                     AFAKAGIAKPPRTLDDVKTAAAKLRQTDDKGRTVFWGIANVPTTYELMVWLGHQNGLT
                     YLTDFENGHIGAPTRVLFDENGTMVNFLTKWQAIYDTGSLANLTSDLNGAFASGQVAM
                     ILASTSNLSTIVSMVGDRFEVGVANMPMVDENATGGVNVGGGAIWAFDNGKNNQWAIW
                     EFLKFATSAEEQLKWHIATGYFPVNVDTYELPEFKSHLQEHPLFGVASRQLMDSNPKL
                     QGIWVPSAYQIYYAFQSGILKMLEEKQTPQAASKAIAKEINGYLQEYHLMKAR"
     gene            complement(69109..69720)
                     /locus_tag="PLF67_00335"
     CDS             complement(69109..69720)
                     /locus_tag="PLF67_00335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE88488.1"
                     /translation="MLFHWISRIYGWFFHYQKRRFKAILTEHGSELESVESACDVGCG
                     TGALCAVLAERGIRTTGVDSAKGMLAVARRKTRGLPIRFILADGVSGLPFLDKSFDLV
                     ISSHVAHGLKKPDRIRFYRELKRIASKTVIIHDYNSRRHPVSTIIEWAEGGDYFNFIK
                     EPKKEMEEIFPSISIVDVSPYFRWYVCRCATEPSSGDTPASSR"
     gene            complement(69722..70168)
                     /locus_tag="PLF67_00340"
     CDS             complement(69722..70168)
                     /locus_tag="PLF67_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOE88489.1"
                     /translation="MEQHIYRIGELAEKCNVTVRTIRHYESLGLLKTVLRSPGGQRYY
                     TDKEVVYLNRITELKELDFSLREIGDIIRLAGEDSTGERRRLELLRQYRNKLSEVLER
                     RAEINGRVEDLSWRIAQLESTASFQECPGLECLECTFKEKCRFKDS"
     gene            complement(70422..71801)
                     /locus_tag="PLF67_00345"
     CDS             complement(70422..71801)
                     /locus_tag="PLF67_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HOE88490.1"
                     /translation="MGDVSVVRVVVGLAVAIGMIFWFVTKTKIHVFLAMIMGSVVAGL
                     IAGLAPTAVMSAIKSGFGGTLGSIGIIIGFGVMMGAIFEKSGAATRMAITFIRILGKG
                     REEIAMAITGFIVSIPVFCDSAFVILSPLSKSLSRNTGKSVITISCALGAALLVTHSL
                     VPPTPGPLAVVEFFKLDLGKFILISLLASVPITVAGVFYARWVGTKKVFKLVDPDDSS
                     KLIDRPYTKEEMATTANISTEGLPSAFISFAPIVFPILLILLNTITSAMGMKGTFKSI
                     AGLIGDPVVAVAIGLLIAIYGLAGKVSRKEVVSMLDGSIREAGVIVFVTGAGGALGQV
                     LKDSGAGAAIANVLVNANIPGILLPFIMAYLLRIVSGSATVGMLTAGSLTAPILLSMA
                     NVNPYIGGLACCIGSIGITNLHDSYFWVVNRTTGLTDVKEMFWNWFGAVLVLSTSGFV
                     VLLLMNWIF"
     gene            complement(71852..73027)
                     /locus_tag="PLF67_00350"
     CDS             complement(71852..73027)
                     /locus_tag="PLF67_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOE88491.1"
                     /translation="MAEKQSGFFGYVRKDGSVGVRNYVAILPSVVCVNEVVEAIVANT
                     VMTQGIIHHQGCCQTPPDLERTTECLIKIGENPNVGAVLIVSLGCEGVDTDRLEAALR
                     RTGKPVDRINVQELGGTSRAIQAGLDKAQRLIRKIAGEQRVEVDMSHFTMGIKCGASD
                     TTSGIASNPVIGYVADKVVDLGGTVIFGETTEFIGAEHILKRRAKNAEVAADIDRIVI
                     EMENRAKAIGVDMRKGQPTPGNIKGGLSTIEEKSLGAIVKSGTKTIQGVIDYTEIPKG
                     KGLWIKDSPGREIELMSGMAIGGAQIILFSTGRGAPQGFPVVPVIKICGNPITYERMI
                     HDMDINAGLITTGERSLEEVGEEVFEMMLRVASGEVTKGEAIKYSKSMDFYMLGPVI"
     gene            complement(73030..73365)
                     /locus_tag="PLF67_00355"
     CDS             complement(73030..73365)
                     /locus_tag="PLF67_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOE88492.1"
                     /translation="MVKAIKLHDSDNVASVFDSVVAGAVVQIIDKKGASFELVVSQDI
                     PYGHKVAIAEVGIGEQVTKYGEEIGIASVPIHVGEHVHVHNIESIRGRGDWDESQAEK
                     VKAGIRKEV"
     gene            complement(73366..74649)
                     /gene="larA"
                     /locus_tag="PLF67_00360"
     CDS             complement(73366..74649)
                     /gene="larA"
                     /locus_tag="PLF67_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOE88493.1"
                     /translation="MKTTIELPYDLEKIRLDIDDQRLAGVLVPPPAKKQNDAARSGSE
                     LVREALAHPVGSDRLRDLVRGKNRITIITSDHTRSVPSAITLPLLLEEIRDGNPDADI
                     TIIVATGLHRGMTKEEMNAKFGSRLVAKERIINHDAFDADMFIDLGVLPSGSKCEINR
                     LAIETDLLVAEGFIEPHFFAGYSGGRKSVLPGIASQDCVNINHSAMAIAHPKAITGVL
                     DGNPIHEDMITAARMANLAFILNVLLDEDKNISAAVAGEVEQAHRLGCSWLQEAIGID
                     PIMADIVITTNGGYPLDQNLYQCPKGLSTALATANEHAAIILVAACKDGLGGDNFAKL
                     MRSGSPDELMEKILATPPEKTVSEQWCVQRFAEALQRHPIILVSELDADTVRAMGFIH
                     AHSVEQALEEAFRLKGESANVTVIPDGVSVIIRSQ"
     gene            74824..75495
                     /locus_tag="PLF67_00365"
     CDS             74824..75495
                     /locus_tag="PLF67_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE88494.1"
                     /translation="MSIGTNKKVILTIKDQVYQIIRDSIISEQLKPGDRVNEVQIAKD
                     LNVSRSPVRSAINELIGEGLLESVPNKHVKVRKLTEKEILDIFELRLLVEGYAVEKAI
                     ERLDDRMKATFRDFRDRLKACNTYAMLGKYVEIDTELHDFLIKASGNEMLHETMHRVA
                     LLINPFRVVSLKSETRFIESIDEHIGLIDALCEKDLKRAKERIETHLTLAKNEVVEYL
                     KTRHN"
     gene            complement(75583..75846)
                     /locus_tag="PLF67_00370"
     CDS             complement(75583..75846)
                     /locus_tag="PLF67_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOE88495.1"
                     /translation="METGSEETMTRFIATFYSQYGAVQFQKFARKHHIECKLAPVPRA
                     LSSSCGTCAHYTSDTWDIGFVKKDLEAIYEATKDGYMTIFSDE"
     gene            complement(75819..76025)
                     /locus_tag="PLF67_00375"
     CDS             complement(75819..76025)
                     /locus_tag="PLF67_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOE88496.1"
                     /translation="MEIDARGLSCPQPIIETKKVIDKKVTSLSVLVDNIAAKENVSRF
                     AKAMKYEVVASETEYGWKLDLKKQ"
     gene            complement(76027..77094)
                     /gene="yedE"
                     /locus_tag="PLF67_00380"
     CDS             complement(76027..77094)
                     /gene="yedE"
                     /locus_tag="PLF67_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOE88497.1"
                     /translation="MKISKEKQSLIIAGAITGIISVILVLLGNPTNMGFCIACFYRDI
                     AGALSLHSAAVVQYVRPEIIGLLLGAFLISLVKKEFKPRGGSSPVLRFVFSAFVMIGA
                     LVFLGCPFRMVLRLAGGDLNALVALFGFIAGIIPGSLLLNKGFTLGRSYKQSKVEGGM
                     ISGLFLGLLVVLLFIPSLLKFSESGPGSMRAPIIISLVAGLVVGALAQRNRFCMAGGV
                     RDVFLFRDSTLLTGLVLVFVSALVMNLVTGNFTPGFTGQAVAHTDWLWNFLGMALVGY
                     GSVLLGGCPLRQTILAGEGNVDSVMSVLGMLAGAAISHNFGLASSGKGTTVAGRIAVI
                     LGFVVITTIAFVIIQAKKKEA"
     gene            77180..78322
                     /locus_tag="PLF67_00385"
     CDS             77180..78322
                     /locus_tag="PLF67_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOE88498.1"
                     /translation="MNTIYLDNGATSWPKAPTVASTMASFITEEAVNIGRGGYDRAYE
                     TAGRIDECREKLGAFFGAPEDRNVTFSLNVTHALNTLIAGLFTKDDHVLVSGMEHNAV
                     MRPLSHLDIPYSIIPCDREGRIHLEQIPHLINGKTKAIIMTQASNVTGTIQPIKELSS
                     IAGKYGLLLLIDAAQLPLSNPLSIIKDNLDAIAFTGHKSLLGPQGVGGLVLSDRLAKE
                     VKPWACGGTGSKSDLLEMPDFLPDKFEAGTQNLPGIIGLSAALDFIKDNSESIIKNEY
                     ETTELLLSYFLSDKRIQVIGPKDMKDRTSVISVDFPTLDNARIAYELFLDAGIETRVG
                     LHCAPVAHKTIGTFPQGTLRFSPGYATTKEEILRTIESCKRVLDRL"
     gene            complement(78484..78744)
                     /locus_tag="PLF67_00390"
     CDS             complement(78484..78744)
                     /locus_tag="PLF67_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88499.1"
                     /translation="MVIKHKDEIMWFIIAFVVVLILVLAFKSNKKEETITTTDQETGK
                     TTVETRVTNTPSAGQTAARIVLLIVAIIVAVLFLLVVCASMM"
     gene            78971..79786
                     /locus_tag="PLF67_00395"
     CDS             78971..79786
                     /locus_tag="PLF67_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE88500.1"
                     /translation="MISVHGLSVGYGKRTILHDVDFSAESSQIIALIGPNGCGKTTML
                     RTLSGQIKPQKGSVSICGQELSSLRQKELSKCVAFLPQRHEVVHGVSVHELVAMGRAP
                     YHRSGWILSMKDKEKIKWALSYMNLEEYVSRPVDQLSGGEQQRVWIAMALAQDTPIIL
                     LDEPVTYMDLRFQRELLSTIADLRDTHGKTIVSVFHDINHAIEVSDHIYLLKDGRVHA
                     QGTNEEVVTEDNIGDVYGIHAHVCRFNRCRRNVVVPSSLNRGFERKHAHRVVM"
     gene            79826..80761
                     /locus_tag="PLF67_00400"
     CDS             79826..80761
                     /locus_tag="PLF67_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88501.1"
                     /translation="MRNIVVLFIVTGMLVMPLFAQGAKEQPVRTEVETKVVIDELSRS
                     VAIPQNPQRVVALTSAVMQSLYNIGIPPVGKVDEYRVTKEGMALPSVGKTSAINIEAV
                     YALNPDLIIASSRFHSALKEALEQSGAAVYFFDPDKVGEISVVDLNIYLGKLLDREKE
                     GRKYVESIYSLARDLRTDIVEPLGIGSAVMIKDGDTITAAQSASSYGSMLKLLGLRNI
                     VPDNLPGSGRGSFVKYSIETILERHPDVVFILASSNDEASNRAMLEKFRNDEQWKHYT
                     LQGGRFVLLPFLANPNRTSVENMLRITADALKKND"
     gene            80770..81777
                     /locus_tag="PLF67_00405"
     CDS             80770..81777
                     /locus_tag="PLF67_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE88502.1"
                     /translation="MKRFHSHRLGVLGAAAILLVFIIALSLSVGSVQVSLGKIVGILF
                     GKKGEGLPVQIVRNIRLPRTLVGLLVGMNLAVSGVLLQGIIRNPMASPNIIGVNAGAG
                     LAAVVVMALFPSRIGFIPPASFIGALGAALTISALANSKGGKRSTVYIVLAGVAVSNL
                     LNAGTSALMVVYSDILEVSYSWLLGSLSGRSWSSFALIWPYSLAGLAFALFLSPKINL
                     FSLGDDVASSVGLSVRLYRFLALLLAAVLAGSAVSVTGTIGFVGLIAPHAARLLVGND
                     HRYALPLSALLGAILLILSDTFARTLFSPVELSVGIITSLLGAPFFLFLLFRKRKASV
                     L"
     gene            complement(81779..82576)
                     /gene="zupT"
                     /locus_tag="PLF67_00410"
     CDS             complement(81779..82576)
                     /gene="zupT"
                     /locus_tag="PLF67_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOE88503.1"
                     /translation="MDNVLFAFSLTLFAGLATGVGSLLAFLSKKFNPKFLAGALGFSA
                     GVMIYVSFVEIFVKARDSLSIPFGETKGYWVTTIAFFGGIALIAIIDRLIPSYENPHE
                     IGNINEPVDKASEQRLLRLGVFSALAIAIHNFPEGLATFMGALSDPTLGISIAVAIAI
                     HNIPEGMAVSVPIYYATKSRKKAFWLSFASGIAEPLGALVGYFILKNFISDSTFGIVF
                     AGVAGIMVYISLDELLPTAEEFGEHHIAIGGLIAGMLVMAISLLLFA"
     gene            82827..84023
                     /locus_tag="PLF67_00415"
     CDS             82827..84023
                     /locus_tag="PLF67_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOE88504.1"
                     /translation="MRIAVAGIGYVGLSNSVLLSQHNEVVAVDLLQSKVDMINKRISP
                     IVDAEISEYLAHHDLKLKATTDGDSAYRDADYVIISTPTNYDPDKNFFDTSSVEAVIR
                     QVMAVNPSAIMVIKSTVPVGFTTEVRKKYGSENILFSPEFLREGQALKDNLYPSRIIV
                     GCPLDDARLKEAAHTFASLLAEGAIRKDIPILIMNLTEAEAVKLFANTYLALRVSFFN
                     ELDTYAEIRELDTKSIIEGIGLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPQ
                     NIMTAIVDANRTRKDFIAERILQKVGFASGIVVGIYRLTMKTGSDNFRNSSIQGVMKR
                     IKAKGVRVVVYEPALNEDEFFGSRVCKNLDEFKQLSTIIVTNRYTQELEDVRDKIYTR
                     DLYGRD"
     gene            complement(84046..84804)
                     /locus_tag="PLF67_00420"
     CDS             complement(84046..84804)
                     /locus_tag="PLF67_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE88505.1"
                     /translation="MRELGLAGMEVRTVDDVNVMDLPERTLREIRKQADDRGITITCV
                     SSPIGKQPVSISPSIVVEQVEKASEIAHMLGTEYIRIFSFFNEQDMTIDEAIGLSSEK
                     LSLMADRAKRTDTVLVMEGGHNTVGQTGKVSRQLFEAVDSAHLRCAFDGAAFVGAGEQ
                     PFTDCLPLLMPYIEYMHIKDAKFNSPRRVLPGDGDVELERILNAVRNKDLVLSLEPHL
                     AYAGPNRGFSGEELFKQAHKALMDILNRLNIPFF"
     gene            complement(84934..86001)
                     /locus_tag="PLF67_00425"
     CDS             complement(84934..86001)
                     /locus_tag="PLF67_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE88506.1"
                     /translation="MARIKTAIIGCGKNANELHTPILLRHPEFIVTGTYDLVYDRAEK
                     LAKVFYLNGHTDCKAYESSEALLATDVEYIVVLTDSVSHTKVAADCLRAGKNVLITKP
                     WALDSKDAAFIIDEAKKAKAKYGVSLMPFLPQNWGTDLSTIKEIIDSGDIGQVYMIRR
                     RAGTFGKRYDWQIYKEFGGGYLNNWGPHLLGQLLALVDEPVVQVFAEKRQVIMDGDCE
                     DTFVAMMKTQSGIILNCEHCIMVDKLPHWIVQGSKGTIFVTDGTIDVHKISYPGPIDP
                     TAYRSKTEDDNKTYPLPGQYNGDRFAIYSHIAEVIRHKCEYRVPLTFTRNLTTVCDAI
                     HASADTEHIVHISSEQWNKAL"
     gene            complement(86014..86814)
                     /locus_tag="PLF67_00430"
     CDS             complement(86014..86814)
                     /locus_tag="PLF67_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE88507.1"
                     /translation="MRYGMCTDIATATRDRVEYELLKRIKEAGFDFVEFPLMMIETLS
                     DDDFEVLLAELKRLGLACDCSCNFFPGRIKVTGPSVNKAEIDAYLDKAMGRAQRMGVK
                     KIVFGSNNSRNIPDGWGLERGYDQMCEVIEELIIPYCRKYGIMIVMEPIRKQSGNILL
                     TVKDGEYFVNRINKPEIRLLADLMHMQYNEENPEDLRDVRPMLHHVHICEMDRMLPED
                     GFSEFLQKALKILKELGYDETISFESKGGTGPDSLKNALTQLKRQFCS"
     gene            complement(86841..88142)
                     /locus_tag="PLF67_00435"
     CDS             complement(86841..88142)
                     /locus_tag="PLF67_00435"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOE88508.1"
                     /translation="MNTMTMGIIVLSGSFIFMLLMGVEIAYSLGLAAVFTCLFMDINL
                     LVVFQAIFYKMANYSLLAVPCFMLMGEFMSIGSMATNIVGLANVLVGWMPGGLAMVNV
                     VDSMFFGGISGSAVADVSSIGSIVIRLMKEAGYDEDFSVALTCTSSIQGIIIPPSHNL
                     VIYAVTAGGVSIAALYMGGYLSGIMLGLCLAVYCFIVSKRRKYPVAEPFKWSNFVRAL
                     WKSLPAIGCMIVVVGGVLFGWMTATESAAAACVYCFIFTFVIERAGTRRDLYEAFSNT
                     IKTIGSVLILAACATAFSWVITYLRIPQIVTAGLFSITENKVLMLLIINGILLIMGMF
                     MNMVSIIYIMVPILLPVITKLGMHPVQFGIMLILNLGVGLLTPPVGQVLFVGCSVGNI
                     SMERLTKALLPQLAFMTVALVLITFWPPLTMWLPRAVGYIV"
     gene            complement(88139..88639)
                     /locus_tag="PLF67_00440"
     CDS             complement(88139..88639)
                     /locus_tag="PLF67_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOE88509.1"
                     /translation="MLEKIRKAVYNVANWICIVALFLIIVVLLIVIIGRYFFNVTPSW
                     SEEFSLFLLVWVGLFSSCIAEYHRTHVRLSFIDTMFPPKLLRAFGIIRYFLKLVFFML
                     MTYYGLLIFITTKQRFGAINLSFRWEVLPGLLTGVFCLLFLLFDTKRIFTDRHEHDAE
                     ELEVFE"
     gene            complement(88922..89932)
                     /locus_tag="PLF67_00445"
     CDS             complement(88922..89932)
                     /locus_tag="PLF67_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOE88510.1"
                     /translation="MKKVVALLFVVLATFAMLFAAGAKEAAATEKISMSIPEINADSD
                     LACQMLVKFGELVEERSNGRIEVNVFGSGQLGGEADTMEMLRLGTVSIIRINPANLAN
                     RGIKIPEYTALGLPFLFQSVQGGLDFLYSDYGKSLGDLVLEKSDGTIRSLYNYICTPA
                     RNMFTKKPANTLPDFKKLKIRSETSDIKIDMINCWASATPLAFSEIYTALSTGVLDGC
                     ENTLSGMRNNNWYEQVKYILETEHVVGSSVFLMNEALWNKLTKAEQEMLTASLKEACD
                     WFQAETEKQIAENKAFLTGKGVTFTPCTDKQAWLDSCKGLYAKYASNVRPDFISTIQS
                     YK"
     gene            complement(90136..91308)
                     /locus_tag="PLF67_00450"
     CDS             complement(90136..91308)
                     /locus_tag="PLF67_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE88511.1"
                     /translation="MTTSADGKTTIFLLVEASYIIPFWFARSIEGLESVATKQKYTVK
                     QVASVKDIEPSSNAIVILGTTKNWIRETISAARRRAFRIILIGAVPGNYGEDISGTMY
                     GSRSTIEAMVRYFHYHNRRRIALVDINSNSSNDVTKFESFLMTSRQLNMNTSYADVYF
                     RDVDSHNPTEHFLMRMEMYDGVICSNDFSAAYIINYARDHNIKIPEQLFVAGLGDISL
                     CRYTCPSLTSATRSYYEAGKQVFDIWKTLNQNPNVESIVTTMRSTIKPRGSTGFLPVV
                     EQDDETGQNDFESEYDGCAPCVANGINAIRAIHCCLAQCDSLDMMIITGVMKNDSNEK
                     LAEELAVAPGTINYRLKKLYKNAGVSTKTEFADLFRLHVSLDALTKDSSELIDNSR"
     gene            91479..92378
                     /locus_tag="PLF67_00455"
     CDS             91479..92378
                     /locus_tag="PLF67_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOE88512.1"
                     /translation="MTAFITNIQRFSLNDGPGIRTTIFFQGCNMDCSWCHNPETLPHR
                     PVLMFYENKCIGCGKCFEVCPEGAHRIVDGNHVVDRTLCTSCGTCTESCFAGALVMSS
                     RKYTVEEVMNEIRQDKLYYDLSEGGVTFSGGEATLHMAFVEALADACHEEGIKVAIET
                     NMAIPFYHIESMLKKMDLIMCDLKHVNTSIHKQFTGLENQMIFNNVGRASLLGIPMIV
                     RTPLIPGATDSIENLHAIAEFVSKLKNILYYELLNFNPLGGSKRIAIDAEDSFEACRP
                     FSDKQLEEIKAKLEDTGVSIKVS"
     gene            92391..94619
                     /locus_tag="PLF67_00460"
     CDS             92391..94619
                     /locus_tag="PLF67_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOE88513.1"
                     /translation="MSKIHYINEFDEDRTFTFDERIALLRKRKIVQTEEKAKQGGADE
                     DDYGLIVQDEFVYQLKPNHPNGSIYGYRAWTENYCSILAEHPIYVDALDAFSAKGFFF
                     LERLRPKTKKWNPDYPYDDLQKIFDRYQIISGIDNCHHFTPDIQIGLDLGWGGILRKL
                     KEQRALHDETHHEFYDSEIAVVEHIIAFIRRAGDEIEALACQEKNRQLAENLHTMAAI
                     NHRLATEPPKTFREAIQWNNWFSMLSRTYNRGSSGGQIEDLFNPFYERDVASGILTDE
                     EAIFYLACMFLQDSRYWQLSGPDEHDNDKTCHLSYLALDAADKINIATNLTIRVHDKL
                     DPAFFEKSVEYLFKNKQGWPRYSSDKALMEGFMRCGYSKELARKRVAAGCHWMCIPGM
                     EYTMNDTVKVNLAMVFQVAFQDMMANADQTRPSIDALWEQYRKHLKMAVDATGRGIIH
                     HLTHQTKSQPELILNLFQHGLIEKGVNITDGGANYYNMCIDGAGLAVAADSFAACQQR
                     IDKEGRLTYEELTNHLDANFEGENGEYVRQMLFHSERYGGGGDSLGDKWAVKISKLWT
                     GLVRDLVEQHKDNPKNVNFIPGFFSWSNTILLGSILKATPNGRKAGEPINHGANPNGG
                     FRKDGAVTSMCNSIAAIQPGYGNTAPVQLEIDPQIANDPEGVKKMCAMILGIMNSGNT
                     LLNINIIDAKKILEAHKDPFKYPDLVVRVTGFTAFFSMLSEKFRELVIERVKAVNADL
                     EY"
     gene            94658..95443
                     /locus_tag="PLF67_00465"
     CDS             94658..95443
                     /locus_tag="PLF67_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88514.1"
                     /translation="MREVPKMTVDFNVKKNGSVALLPGCIPGATLYHHSVMAGSTLSL
                     EPGPFHHILILISGDVEFSTDGNSYIFHERVSFVPNPKLSVDIKALTDCHLIEVRWEW
                     QEGDDELAAEYKTQFPHIQIYRNAKQYRDRNKSEKTISRSVIDQRIIPRFAFGSVETY
                     GVDAIKSHDHPMLDQYFFSFPENEMDVLIDFEPIPMKGNELLYIPLGSMHGVDVKEGQ
                     HCHYLWIDFYPDNELALKRLDASHIPTGKHVEFDPKTGKLKKE"
     gene            95458..96546
                     /locus_tag="PLF67_00470"
     CDS             95458..96546
                     /locus_tag="PLF67_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOE88515.1"
                     /translation="MSYTAPVKLASARAWRTYTGGSKIDEIHGIFDGKDSQFPEEWIM
                     STVVARNVGREEFVHEGLSFLEHSQESLRDFIARNPKDALGTTHFDSVGDTTGVLVKI
                     IDAAERLAVQVHPNKQKARELFHSRFGKTECWHILGGRDIGGRPYGIYMGFKEGITRK
                     EWERCFYEQDIPALLGHMHYFDVKPGETYLIKGGVPHAIGQGCLLIEIQEPTDYTIRV
                     ERTTPSGLSVPPQACHQGIGFERMFDCFEYDGISREEAYGRWCILPKILQQTDDFTKS
                     LLIGYEDTTCFRLERYDMTGKCEIRTDGIFCGLYIYSGSGSIISEDLVQHIKGGDQFF
                     IPAKCDSFTIEAEKDHPISMFRFFGPKR"
     gene            96555..97757
                     /locus_tag="PLF67_00475"
     CDS             96555..97757
                     /locus_tag="PLF67_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE88516.1"
                     /translation="MDDVERRAAEIPGSTIIMAAEYDVVVIGGGIAGCAAALASRRKD
                     KKVLLLEKLTVLGGLATTGHIVIYLPLDDGYGRQVIGGISEELLRLSMNYAYYAGDIS
                     LWKEQGRRYECKYNGPAFSLALEESLLAEGIDILYDTLFVGSEIEDGVCKAVITENKS
                     GRSRYRCKAVIDASGDAEVFARANEPCMAAKNSLAIWSYCTQGADAQYQKRGGPDGPG
                     LSLVSFGKIDTQAFKHTVTVPYYGDTAEGINRFIIDGHARLLNHMKAHDDFVPASLPG
                     MAQIRMARRIVGRYELSDKDAGRHFEDNIGGTGDWRKPGPVYEIPYRTLMTRNLKNAL
                     AAGRCISSSGEAWELTRCIPQAALTGQAAGTAAAIAIERSLSVQDIDIRQLRETLKRD
                     GMILDVNQ"
     gene            97774..98799
                     /locus_tag="PLF67_00480"
     CDS             97774..98799
                     /locus_tag="PLF67_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE88517.1"
                     /translation="MENTVRIGIVGCGMVARYHAKAIAETPNAMLIGACSKSLSSTRT
                     FCSEFSQVRAYNSYDEMLAEHDINTVAVCSPTGNHYEQAKRALEAHKHVVVEKPICIT
                     LRQADDLIRFADEESLSLCVISQTRFSDSATIIKQAIDNGELGRPISASLMMRYMRTQ
                     AYYDQADWRGTYAYDGGGVLMNQGIHGIDLLCYFMGKPQSVTGYAKTMLRSIEAEDTA
                     VAAIEFENQSVAVIDATVCSQPPFPKRFILCGEKGTIILEDDAITLWNLPTPCPIEIK
                     ASAGGSSSADPKGITHIYHAREYRNIVRHLLNGEALLIDGRQGRIPLSVILGVYESSK
                     TGRKVLL"
     gene            98796..99863
                     /locus_tag="PLF67_00485"
     CDS             98796..99863
                     /locus_tag="PLF67_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE88518.1"
                     /translation="MKYALIGCGRISTNHIRAAVHNSLEIAGVCDIVGERMDAVLAKN
                     SLNTDSTIARYHDYERMLKNLKPDLVSIATESGKHAEIAISCINHGCNLIIEKPIAMS
                     LFDADRIIEAAKKMRVKVAVCHQNRFNIAVQKTRKALEAGRFGKLSHGSIHVRWNRNR
                     EYYTQAPWRGTWEEDGGCLMNQCIHGIDLLLWMMGDEIESVYGATRRRFHDYLETEDI
                     GMALVQFKNGTLGTIEGTTNVYPKNLEATLCLFGENGTVKIGGTSTNTIEVWNFVDAS
                     ASENETLIEKTADIYGNGHLHLFADVIDAIVNDRDPYVDAYAGKKALEVILAIYKSQK
                     TGLPVTFPLDDFASTDMIGTF"
     gene            complement(100217..100519)
                     /locus_tag="PLF67_00490"
     CDS             complement(100217..100519)
                     /locus_tag="PLF67_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOE88519.1"
                     /translation="MAARLSCRFAPVTMALTGMPLSVTSSLNIYLLSPLYEKLLHSSP
                     HVDLRIRTHQSPGIYNMINNHEVDVGFVFHQSRYKNVIAEDVILEKMVLLVSSKGD"
     gene            100497..100703
                     /locus_tag="PLF67_00495"
     CDS             100497..100703
                     /locus_tag="PLF67_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88520.1"
                     /translation="MHDSRAAIPLERMTGQRIRHLYSLMDSAYDAKPIRDFITDNGRI
                     PIEVSRACSIHSYHFLVDSRVGDI"
     gene            100738..101754
                     /locus_tag="PLF67_00500"
     CDS             100738..101754
                     /locus_tag="PLF67_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88521.1"
                     /translation="MTHNKTQTGNLFLVILIIMVASCLVGCGAQKKPAQFEEHDGIIV
                     GFSQIGAESAWRTCNTRSIQEAAAERGVQLVYDNAEQKQENQIKALRSFIAYQVDVIV
                     FVPIVTDGWDNVLQEAKDAGIPVLVTDRKIRVEDPSLYSGFIGTDSITEGRNGGLFLL
                     NKFKAERDRFEETGEHINIVEMFGTEGSSVAIGRAEGFRQILDDHPEFRIIYSKSGDF
                     LRSRGYELAVEFLEQYDDIDVIFSHNDGMTLGAIEAMEERGIRPGIDIVIVTIDAQQA
                     AIDALREGKVNCVIECNPKTGPEIIELAEKLAAGESIPKLQYVHEEVFYETDDLSLVA
                     PRGY"
     gene            101756..103228
                     /locus_tag="PLF67_00505"
     CDS             101756..103228
                     /locus_tag="PLF67_00505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOE88522.1"
                     /translation="MKNRMTLIDKIFSAFKIRSIKESIKISYVIIILLMITPPVITIG
                     SFVIQMVRYDLIITNVSKANRLNQTVKMDVSNEIWDIVAGNKKFNEGDQYKIIDGINE
                     SLEDIMSTTEERENRQMLEVAGRAVDTLKRNVDRLGSQMASSSPVSENEEILEEIRGV
                     SALISDILQDFIVRVIESATITNEHHKRITLVLTVIQVFTVFLVAIFAVFTQRSVTAS
                     INDPIKRLEHLSKRIAEGDFTARVQLPQVSELDGLTDNLNIMAVKIQALIEENIRKQQ
                     LIQKSEMKALQAQITPHFLYNTFDTIVWLSEEKKNDQVIDITRSFSNFFRISLNKGKD
                     FLTVREEFEHVKSYLSIQKIRYRDILDYEIEYAPEMAECQILKLVLQPLVENALYHGI
                     KNKRARGFLSVKGWRENGYLCFSVEDDGIGMTEEKLENIMKQISSSTNPEDLSSIYGL
                     YNVNKRLELYYDATTKLDITSRYKQGTTVFFKVPEAGFNV"
     gene            103221..104810
                     /locus_tag="PLF67_00510"
     CDS             103221..104810
                     /locus_tag="PLF67_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE88523.1"
                     /translation="MYKVFIAEDEIVVREGLRNTIQSGTGPFVLAGEASDGEMALSIM
                     KDVKPDILITDIRMPFVDGLSLCRIIKKILPWIKIIIISGHDEFQYAQEAISIGVDEY
                     LLKPISASDMLETLNKLVDRIEQEKKHLSSIEHLKLQAQSSSDLIRERWLSDLVTGIV
                     KTEDALESAGDMGIDLIAHSYLVAIIKLSTSLENYSDLITAKLHINSLIDNQEEVLCF
                     SQSRDTIILLLMQLVSESLEETAYTLGQAIKYEVERNTRCMVAFGIGSLVERIGSLSQ
                     SYADAEKAIHFSVKTGQKLIIGTHDLSSFSEIDFLKLDGSSISERLKYVKKSGVDEII
                     AQYVTMIGDQPLQTTLIGYYLLYDLMVAISKIIDELGGDVQEVIPSLPQKTQLSEMAA
                     SKETFCDGVKLILDTFIEFRDSRSAGKYYEMIQKAKQHINLHFADQDISLHSVASIVH
                     VSPNHFSTIFSQETGETFIEYLTGVRINKSKELLLTTALRSADIAYEVGFGDPHYFSF
                     IFKKHTGISPREFRTGNKAQT"
     gene            complement(104820..105899)
                     /locus_tag="PLF67_00515"
     CDS             complement(104820..105899)
                     /locus_tag="PLF67_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608103.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HOE88524.1"
                     /translation="MASEKAIKQKARLSNSNLLFLIASVIFVLMYLFAIISYPGSFLQ
                     FQTFFDLFNLNAPLIIMTFGLCIVMIGGGIDISIGSVCGLVTMACAVYLEKRSGSIAG
                     AIFLSMGIGIVFGILHGYLIAYLEIQPFIITLSGLFLAQGLLTTLHKDPLNVTMPAFV
                     NLRDFNIVITWLGTRNRLGVFIPCQIKPGTIIVAVLLVIFASLMKWSRFGRNVYAVGG
                     NSHSAMMLGINVKRTKFITYVISGLTAGIAGFVYIMTTGAGNIGNAAGAEMKAIASAI
                     MGGTLLNGGVGNLLGAPIGTLTLLVINELIRAAGVQSNFQAIVSGLLLYLFIILQSII
                     MSLRDRKRFTVALPPWLRLSGKGQA"
     gene            complement(105901..106929)
                     /locus_tag="PLF67_00520"
     CDS             complement(105901..106929)
                     /locus_tag="PLF67_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88525.1"
                     /translation="MRKLMKNVSHLVLPISVMVLLIIINLVKGADYFTITMINGALYG
                     NIPNILFGASELVILSIGMTLVTAASRGQDISIGESATISSAVFVWFVLRSGEATPVT
                     IIVGFLLSCVVGLGLGAFNGILVSIFKVQPMVASLILFTGGRSIAFMIDGKLSPILAN
                     DISNRIGTVIPGIPIQTPVILTVVFIALFAVLLKTTTLRLYVETVGINPNAARLNGIN
                     PKKVIFLTFLIMGFCSAVAGFIAVNKAGRHDSVNLLKNIMMDAILAVAIGGNSLGGGT
                     FSITGSIIGAYTIEMLNRTLLRLEIEPAMIKVFKAIFIVILMVVASPVVRDFIKKVLD
                     RNRTRKEE"
     gene            complement(106929..108434)
                     /locus_tag="PLF67_00525"
     CDS             complement(106929..108434)
                     /locus_tag="PLF67_00525"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE88526.1"
                     /translation="MLSETILEMKDISKSFPGVKALDGVDFKLREGEIHALMGENGAG
                     KSTLIKVITGVYEKDAGLITLHGKPIHFRSPQEAQNNGIGTVYQEIMLCPNLTVAENM
                     FIGRSHAPIVNWKEMNEKAARLLDSLGIPASPIQELSTCSIAVQQMIAIARAVDMDCR
                     ILILDEPTSSLDEDEVQKLFELMRALKAQGVGIIFITHFLEQVYEISDRITVLRNGKL
                     IGEYKTSSLPQIELISKMLGSELEDISTIRKHEPVASDAGIPVFEAVGLSSAAGVRPF
                     DFTIHKGEVNGFAGLLGSGRSESVRAIFAADKVTGGEVRIHGKRVKIKTPLHAIKHGI
                     GYLPEDRKGDGIIEDLSVRDNIILTLQVLKGLFKPFSLKEAEKFAEEYIKKLNIKTPT
                     SNTPIRSLSGGNQQKVILARWLLTNLEYLILDEPTRGIDVGTKVEIQKLVIRLASGGM
                     SLTFISSEIDEMLRTCSRLIVMKDREIVGELRGTDLTENDVMHMIAQGGKS"
     gene            complement(108517..109503)
                     /locus_tag="PLF67_00530"
     CDS             complement(108517..109503)
                     /locus_tag="PLF67_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE88527.1"
                     /translation="MKKTLCVLMVLVLLGTGVLFAAGQKETGLTKVGIVNLPPEESGY
                     RQANVEDMNNVFSKANGYDSKQTNTMDNSEQIAAAKGYIRDGVDYLLISAANASGWDD
                     TLKSAKEAGVKVILFDRAIDTDPSNYQAALLSDMAYEGEKAVEWVLSLGLSKINLILI
                     RGQMGSAAEIGRSKAVLDAAKAGKLNIVADGTGGDSWSLEEARKVVEAAIAAGKDFNV
                     IYAQNDGMAQGAVQALEAAGISHGKNGKVKVIGFDFNRFALRNVQAGYWDADMQCNPR
                     QAATISEWIKSGNIPSGIVYQEELLFTTDTITDADIEKWGINDDPGKGVITR"
     gene            complement(109856..110614)
                     /locus_tag="PLF67_00535"
     CDS             complement(109856..110614)
                     /locus_tag="PLF67_00535"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE88528.1"
                     /translation="MKELLAGRVAVVTGSGRGIGAAIARRLAEYGAKVVLTDINTEGI
                     ETLEKDLVAKGYEAKSLMFNVADFDNIPKQVERIHALFGSIDIWVNNAGITESASIES
                     ITQQQWDRMQDINLKSVFFCSQAVFKIMKEQRYGRLVHISSMAGERGGRGSSASYSAS
                     KAGVINLAKSFALNGGQYNITSNAVCPGRTLTEMAKGLSWLTDPKDDPKLTIPLGRFG
                     VPEDIADAVLFLASDLSSYITGIALDVNGGLYMR"
     gene            complement(110633..112111)
                     /gene="glpK"
                     /locus_tag="PLF67_00540"
     CDS             complement(110633..112111)
                     /gene="glpK"
                     /locus_tag="PLF67_00540"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOE88529.1"
                     /translation="MEKRYIVSIDQSTQGTKAMLFDEQGKCIASEVVAHRQIIDRRGY
                     IEHDGMEIAKNVHLAVRAILEKSGVDRSRIVAMGLANQRESVIIWDRVTGRPVYNSIV
                     WQCNRAKDICDRLADVADYIQSTTGLRLSPFFSGPKIAWVLENVPGVRQKAEQGRLCC
                     GTMDSWVIYNLTKGSSHKTDYSNASRMMLMNIRTGTWDDGVCSLLGIPKSMLPEICDS
                     DASFGFTDMDGLFEKPIPIRCAIGDSHASLFGQGCYEKGMCMTGYGTGSCVMMNLGDT
                     PIISKHGVLTSIAWKIDGHVRYIFDGVINYSGAVITWICKDLGLVSSPQETCSLAEQA
                     NKEDRTYLVPAFSGIGAPHWKNESNAMFYGMSRLTGKAELVKACLESIAYQVSDVVSA
                     MSLDAQVAIPFMKAAGGPVKNRYLMQFQSDILNCPVHVPDHEEMTCFGVAMIVGQALG
                     LYDDRTREALDIYHEYQPTMSSEGRNRCIEGWNRALRCVLEN"
     gene            complement(112134..112850)
                     /locus_tag="PLF67_00545"
     CDS             complement(112134..112850)
                     /locus_tag="PLF67_00545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOE88530.1"
                     /translation="MKTRIYFIRHGETDYNVQHRFQGSTDNPLNERGLEQASCLWSPM
                     SKFHLDKIYVSPYKRTMQTAKQVLAGRDIPVIPEPKLSEIHCGQWEGLVRTEIEERWP
                     GMIDLWEHRPDELHMPDGESFKQVQDRSVQALKEILEKEAGKSVAIVTHMLTIQLIMS
                     KLLGIPIREVWKMVRLENTSITTIDFSSNKEFEVIKWGEDSHLHEHLKNSYVRIAGFV
                     QKDHAKYDTSVIEGKHPFTL"
     gene            complement(112865..114229)
                     /gene="lpdA"
                     /locus_tag="PLF67_00550"
     CDS             complement(112865..114229)
                     /gene="lpdA"
                     /locus_tag="PLF67_00550"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOE88531.1"
                     /translation="MDTYDLLVIGGGPGGYACAIKAAKASMRVAIFEKESFGGTCLNV
                     GCIPTKYLLDKASGLEKIRMLTEAGIYKDAGMFNFKEIQRQKTKVVEKLVGGVNGLLK
                     HHNVAVFKGEAILKADKVVECNGKRYTANHIVIATGSRPAMISIPGVEHAITSTDLLA
                     IPSIPKRLTVIGGGVIGLELSSAFNSFGSKVTVIEMMDSLLPKEQPQAGTQLKKTLEK
                     NGLTFHLSARVNRIEKRDGVCRTIFTVGDKEGSIESELVLIAVGRKPNTDCITPDSKI
                     VLTDSKQVKVDRYLQTSVDGVYAIGDVIGGYQLAHAAYAEAESVVDTILAKDTKAPYD
                     DSVMPRCVYTIPAFAAVGKTTEQAGVETVVGTFSYEANGMALAEEAKGSVYVIMDKQH
                     RITVGVQIVGEHAPELISLATAAVAKKFTFNDWKHLVIAHPSLSEMVKEAALDAFKSA
                     LHKV"
     gene            complement(114243..115448)
                     /locus_tag="PLF67_00555"
     CDS             complement(114243..115448)
                     /locus_tag="PLF67_00555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOE88532.1"
                     /translation="MIKEIRMPNAGQTTDAAVIVEWKVKLGDKIQRGDVLLEAETDKA
                     VLPVESFTAGIVIDILAQEGETVEGGDVVCVIGDAKDVASYSRSAESETTQTTQDRIV
                     TQEGQDDEFIPIMKRPLEKPALPAEAECFPAMPNSKRLAKELGVELGTIKPANGVYIT
                     RQDVLAAVKDRPHDAAAYTVMPMGRMRKAIARRMMESVSTIPSFQVTISVDARQVLDL
                     KKQLEVLRQIKVSYNDIITKAVSVVAKDFPMVNARYEHDEIRVYEHTNVGLAVAIEDG
                     LIVPVIRNVDMLSIQEIAKTSRELIEKARKGILLADEMGSGSVTISNLGMYGVDHFTA
                     IVNPPESAIFAIGSIEMKPVWDGSNFKPIEMMTITGSFDHRMIDGAYAARLLDALRTL
                     LEHPLMMFM"
     gene            complement(115450..116463)
                     /locus_tag="PLF67_00560"
     CDS             complement(115450..116463)
                     /locus_tag="PLF67_00560"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOE88533.1"
                     /translation="MALLSIADALKSAIAEEMRRDPLVFCLGQDVDIPGGMGGAFTVT
                     KGLGEEFGYERVLNTPISEIMLAGVSVGSAMMGMRPVADLQYSDFLFCMMDQLVNQAA
                     KMCYMSGGKVHVPMVMRAPCGASNRGAQHAQSPESLFTHVPGLKVICPSTAYDAKGLM
                     KQAIRDDNPVLVLEHKLLYGGKRKEKDAISTIGEVPDEDYVIEFGKAAVRREGKDITI
                     VANLLMSYKTLEAAELLEQEGISCEVIDPRTLVPFDYETVVNSLKKTGRLMIVHEDNY
                     NCGWGAQLGSWVAENQIFLLDAPIVRVAAYDTPIPFSPVLENYVIPSKERIVEEARKL
                     MKV"
     gene            complement(116466..117443)
                     /locus_tag="PLF67_00565"
     CDS             complement(116466..117443)
                     /locus_tag="PLF67_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOE88534.1"
                     /translation="MSKLPDKERMLGMYRQMYLIRKYEERIYYLFLEGIMPGTIHQST
                     GQEACAVGMLYDLRKDDCMASTHRPAGHDLAKGVSLKSMMCEMFGKEEGCCRGKGGAM
                     HTGDMEVGALVANAIVGGNLPIAAGVALAFKMQKKDNVIVCFFGDGATNEGSFHESMN
                     AAAIWKLPVIYVCENNLYSATTSIALTCNMPNPAADRAASYAIPAEIVDGNDVLKVNE
                     AATRAINRARAGEGPTIIELKTYRHGGHSRNDACQYRPKDEEKHWFDRDPVKNFRNYL
                     ISKKLVVEQELKTVEKAIELEIDEAVEYAQNAPFPQIESALTDVYWEGK"
     gene            complement(117667..118488)
                     /locus_tag="PLF67_00570"
     CDS             complement(117667..118488)
                     /locus_tag="PLF67_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE88535.1"
                     /translation="MKIVFISDIHFSPGQDWSQCIWKYTPNEELPGLLDCFVDIVNLE
                     LKPDMIVELGDRIIDVDETRDIDNTRSIHRMLDERIACPVFHVDGNHDFVHIGKQKLA
                     KLLHNPILPFVRVFKGYKCIFFDSLDPMLAGVGGSVSDEQLAWLEHEMNNDDLPKMVF
                     SHHPLNHHEIHRNFLIPQETVPLMKLSNSKEVISILENGKNLLFHAAGHMHWWSFRVS
                     PKGTYLVNPPFSAAHPERTNAPGWFMEVDVRPDGKVDAKVHSIVPRRVVGDFTNR"
     gene            complement(118516..119352)
                     /locus_tag="PLF67_00575"
     CDS             complement(118516..119352)
                     /locus_tag="PLF67_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOE88536.1"
                     /translation="MFDCVNQVAVCAHRGDCVHGMQNTMTAFRYAIGLGVDMVETDVR
                     MSKDGQLFLMHDPNLEDLTDGTGRISNHTYEEIRSMNVAVRGIASDLVSIPDFEPVAL
                     LEELLDLAVDHPALMLNIEIKDKPDEVDEAFAFECARKTSDMLATRGLGSRTWINSFS
                     GRIVEWVHTIHGDTFHYHGFFPWHIMGSMQTNPWELCDVACVLNWVLRTDGSVHKQHT
                     VPCPAAWYKQLLRIKVMPLTVSFYGDMQAYDDAISYGSRILMADDPAAMLAYVRAKGL
                     HD"
     gene            complement(119356..120687)
                     /locus_tag="PLF67_00580"
     CDS             complement(119356..120687)
                     /locus_tag="PLF67_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOE88537.1"
                     /translation="MNMKKRLLVFSVDALVREDVDKLMTLPNFGAVMKDSCQITHVRS
                     IYPTVTYPIHTSIVTGCYPNKHGIASNFEFTTGDKEQNWLWDHRHVRVEDLFTVAKRS
                     GYSTGSVFWPVTGNHPDVDYLLNEYWMPYAGDTLESSFRRAGSSEEVIRIALKHEDLL
                     PESYHKTGRTNFMVQPYIDNFLIACACDIIEQFAPEVMFVHDGIMDGTRHKYGVFNSE
                     TDKGIEFVDKQFGLLMDALRRAGVYEETNIVVMSDHGQMDTKRIMKPNVYLRDHGFIT
                     TNECGDVVDWEAFCFSNAMSNLVYLKNPSDADTYEAVHRLLKEMAQEGIYGFNEVLTT
                     AEIDKREHLSGDFSFVLESDGYTSFSDDCMRPAIKPFDVTDYRFGRATHGYNPDLGPQ
                     PVFNAVGPDFRRQVVIDRRPIVDVGPTFAKLLGLEMPHCQGTAIEQFLLGG"
     gene            complement(120705..121718)
                     /locus_tag="PLF67_00585"
     CDS             complement(120705..121718)
                     /locus_tag="PLF67_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE88538.1"
                     /translation="MRLKRFLVTALLVICVLFNITALYASEIPVDKDGTYTSAVFDTS
                     QDAGKLTARYLHTTGSRKTANDVTYAGDCTVFTSPEGLIMVVDCSNSWNFEEIDIQLQ
                     RMGVKEIDIFVMSHPHADHIGSFVELASKYPIGHIYKNAHEYESTTYQNAMDKVRQLK
                     IPCEILYTGDSFMFGDRVSVKVYGPDEGAEENVKAGYMTANDLSLAMKITFDKSSFWT
                     SGDLYITKELDLVERFGDELKSDVMKMNHHGYDTSNNSEFIATVDPKVAVQSHSLITN
                     RTVAMRYHLKQKALTFYTCQDGAVAVSTTGDGTYEVQTQYIRQITNIYGKPSANGHYS
                     IRD"
     gene            complement(121823..123202)
                     /locus_tag="PLF67_00590"
     CDS             complement(121823..123202)
                     /locus_tag="PLF67_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE88539.1"
                     /translation="MKKLTVVMILIALILSPMFAKGEVESKVVEVEETKPAGTVEVVW
                     WTFHGKTNVGYFQKVIDAFNASQNDYHVTIEYQGSQNELIAKMQSTLKNELPALFHCA
                     IENIGMVIEADYAVSAQPYIDKDKAGWPELENTYESFYAASCDGDGNLVGYPSGVSYP
                     TIYYNADMFAKAGIDPASLTSFEKVLDASRKLVKGGYAQYGIGFHTDAGYYVNAALAR
                     EGVIAYDNRNGLEGNITKCLYKDGGVVESTLSKYLGFYKDLYKENLGVTFGANYSSEI
                     IPLMAAEDCAMMLGVISMTTKLLTAVDNAFEVGVIPCPSVTEAGARAGEPSGGTWLFI
                     ADNGNKWAMQGGYEFMKFASKGEWAGYFAAATGYLAPSVDAFESEVYQNYMKNIFPGI
                     NTVYQSLEDCKGDALMPICGVSAEIKNANKLAVETVCNDPTDATIAKAIKTAYDQIQE
                     AIDLYNLAN"
     gene            complement(123268..124104)
                     /locus_tag="PLF67_00595"
     CDS             complement(123268..124104)
                     /locus_tag="PLF67_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE88540.1"
                     /translation="MINPRTKKNILSVVSYSAKLLIGLAFISPLIIGLMFSIHTEAEL
                     SQLPLRLFPKVPTLENYAKVFTDVPIFHYLKNSIIVCVVAIVSQIVFGSLAAYGFVFF
                     KFPLKKFLWTVILSTMMIPGEVVIITNYVTVQHMGLVNTYAGLFITSLISGTSIFMMR
                     QYYMQLPKDFKEAATLDGCGDIGFFLRIAMPLSVSTISALAIYQFIRIYNAFFWPLLV
                     TNKDVWRTVQIGVAYLVTGDVEEYGKVIAAAMVAMTPAVIAFIFGQDYIVKGMVSSGI
                     KG"
     gene            complement(124101..124997)
                     /locus_tag="PLF67_00600"
     CDS             complement(124101..124997)
                     /locus_tag="PLF67_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE88541.1"
                     /translation="MFRRNAGYAPCGLKKRVSPLPYLFIAPAMILFGMFTIYPFLRTI
                     VLSFTLTNTYGNPTKFVGLRMWKRVFTSEVFWTVMGVTLKVAAINLVGTFMFALFFAL
                     LSANIVKGSKIYQTMFALPMAIASAPAAGIFVFIFRQKNGLLNSILGTTIAWQHDLRT
                     AIWVVCFVTVWMHIGASFIFLLVGFRNVSTELLESAVLDGANNMQKIIHIMIPMASPQ
                     IFFVLFLNINSAFKSFAQIRLLTGGGPVNSTKTLIYYIYENALIKGRFETACIQAICL
                     FLLIFIVTRIQFALEKKVVHYQ"
     gene            125372..126403
                     /locus_tag="PLF67_00605"
     CDS             125372..126403
                     /locus_tag="PLF67_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE88542.1"
                     /translation="MGLTLNDIAKKIGVSTATISRVINNDNNVREETRLKVEKALADY
                     DYKYRPRRKSTKRSNADAVMVIAGQLSNPITLAYIKGIRDGLEDTPYKVFVSITDYET
                     KNEVDYLQYAKDNSFAGIFMLNVIENESLIRTLNSIESPVILVNRYLRSKDTDIVTVD
                     NYRCGFISTQYLVDHGHTRIAHIAGPSNSITCQNRTLGYMDAINENKLKFRTQDVFYG
                     DRTYQSGYEFGQLFCKIKAEERPTAVFSISGTMAEGFADAVIQEGLSIPEDVSLICND
                     DSSKEYVRKLKITCVEPNMLTIGMAAAELLLERIKNPNSQFRRIVYPPVLTENGSVKT
                     LVQQEAVSS"
     gene            complement(126947..130285)
                     /locus_tag="PLF67_00610"
     CDS             complement(126947..130285)
                     /locus_tag="PLF67_00610"
                     /inference="COORDINATES: protein motif:HMM:NF037501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HOE88543.1"
                     /translation="MTNNEMLNRGFRYTLLTLAPFVVCELKAYYGSDWWELGVLDSFT
                     YDQKKDLPTTGSDDELENSLDILRCLQLINWQWKDIFNKKLAKEQRSWINELIDVRHA
                     WAHAGREDFDNEYTWRALDTMTRLLEPIDTTNIEEIRKLARTVLYGSPEGSAQSVVQP
                     EEVQKAIPKNAGIMREAPIKGLKPWREVIQPHPDVAQGRYQKAEFAADLSQVARGEGS
                     PEYRDPVEFFSRTYVTEGMKGLLAEAIKRVSGKDGEPVIQLKTAFGGGKTHSMLALYH
                     LLRGTVPVDKLPNAKAVLEAAQVTTLPKVHVAVVVGTALDPTKARRPPTMPGITINTL
                     WGEIAAQLTESSGKKDLYNFIKKADNKGVSPGSNALRGMFDAVGPCMILIDELVAYAK
                     KLYGVDGLIAGSFDNLITFIQELTEAVRSSKNSVLIASLPESELEIGGEAGQRVLETI
                     EHTFGRMESIWKPVAAHEGFEVVRRRLFSPCSNEGERDAVCNAFSNMYIQNASDFPIE
                     TKELEYRNRLISCYPIHPEVFDRLYEDWATLERFQRTRGVLRLMAAVIYDLWIRGDQS
                     LMIMPSSFPLDVQQVRDELSRHLSDSWNAIIERDVDGTKSVPFLKDKEIERFGKHIAA
                     RRVARTIFLGSAPSVKQMNLRGIETTRIRLGVIQPGEHIAVFNDALTTLRDSLTYLYS
                     NTNATRFYYDTRPTLQKTAADRATLLNTQDVLDEVENRIRSSQRAESFKGIHIWPSSS
                     LDVPDEKSVRLVVLHPTKIHNQGNEESSALVEAQKILETRGESPRMFKNMLVFAAADS
                     SIKMNLFEEVKMFLAWKSIEEDTEEINLDQTQIKEVQRNLKRHDDTISLRIHEAYSWL
                     FVPGIDVEHDKKTVQWDCLRIGGARESIATRAYRRMVQDEEIIAKWAPPLLMMELNKW
                     FLKEKSFIRVKDLWDMFATHLYLPRLLNFNVLADAIAQGVEGDEYFGLADGISEEGTY
                     YGLKIKKKVESVEDSHYIIRYAEAIAAVKDKTESQIVSTDTEEDFVLSPDESFPADTP
                     ASSQSSGPKSFHLSTKLDTPRYIREIGKINEEILNHLLSIDGANVEIRLHVEVDFDKE
                     VSLDTIRTVTENCRTLKIDDSGFN"
     gene            complement(130297..133065)
                     /locus_tag="PLF67_00615"
     CDS             complement(130297..133065)
                     /locus_tag="PLF67_00615"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOE88544.1"
                     /translation="MVKKKLIEVALPLDAINNASVREKSIRHGHPSTLHLWWARRPLA
                     AARAVIFSSLVDDPSSHPDKFPTEEAQEKERKRLFDILERLVVWENSNDEELLAEAKA
                     EIERSTDGDVPEFLDPFAGGGAIPLEAQRLGLKAHASDLNPVAVMINKAMIEIPPKFQ
                     GMPPVNPEARAKLGSELAWKGSTGLAEDVRYYGEWMREEAFKRIGHLYPTAKLSNGSE
                     ATVIAWIWARTVTCPNPACGCEMPLVRSFDLSTKKGKEHHVVLEIQDNIVTYKVKSGK
                     AKVLKGTMTRTGATCPCCGTFVDLPYIREFAQHEGLGSQLLAIAAEGSNTRLYLSPSK
                     DHSQIAEINRPEDFPSGKLSGKARVSVPLYGMDETADLFTNRQLTALTTFSNLVSEAQ
                     QKAYEDALHRGMEDGLELRNGGKGAKAYGEAIGVYLAFLVDKLADYNSTICSWINTLS
                     AIRNTFARHAIPMVWDYAEANVFSNSAGSLKSMLMWLTKSIQTLPAMLSGSVIQHDAA
                     KDNGLRNIMISTDPPYYDNIGYADLSDFFYIWMRQSLKDTYPKLFSTMLVPKEEELVA
                     TPYRFNGNKEKAKDFFEDGMLQTFRQVYKYTRADIPVTIYYAFKQSEISSGEEDDDNS
                     SSVTVSTGWETMLSAVIQAGFAITGTWPIRTERSVRSVAQGTNALASSIVLVCRKRNP
                     SSSFITRKEFVTTLKRELNPALVKLQQANIAPVDLAQSAIGPGMAVFSRNGKILEADG
                     TEMTVRSALEIINQMIDEFFSEQEGEMDAESRFCVDLFSQYAFNPVKFGTAEVLSKAK
                     NISIDQLVSKGIITAQKGDVYLNERKDLPGFKIGIDYNVWLTTQQVVLAFQKDGVEGA
                     TRIMAQLSGYLVENIKALAYRLYTIAERKGWAGEAYAYNSLVSSWMTIQDRANKAREY
                     IPGEFDFG"
BASE COUNT        32816 a        35462 c        32874 g        31965 t
ORIGIN      
        1 gatcaatagt atagaccccg aaaccaagat acacatatga aggagtccat aaaagagtag
       61 aaaagcatgt aagaacaacc aatcccaata ttggaaagga tattgacgaa gctgaatccg
      121 ttgcccgcat tacaaaagag tccgtcaggg tacaacaatc cgacaaccct ctgaacgcaa
      181 aacgcttcta ttcaaggagt tttccttttg tccagcccca ttcgactccg gcttatatcg
      241 cctcaacctt gctacctgaa tctgtatttg cctttgccat gaccggacac cggttcgatg
      301 acttcgtgtt ccaccagctc tttcaaaagc tccgatgctc tggacggttt gatcttgatt
      361 gctctcatca catccgagcg gccaaagatt gcccgaccgg gaaatgcctc acggagcgtc
      421 agaatatggt ttaccgtttt cggttggaat actggctcaa tgtccgcttt taatccttca
      481 atgtccggtt tttccgtttc tttccatgta ccgctgatat gcaatgtccg attatggagc
      541 ggatggttct catccaacag aagatttctt aagaacaact ccagaaattc cgtcgtttca
      601 tggataccgt ttttcagatc attgtaattc gctcggacaa gggaattgcg gaagaaccat
      661 gcgttttcag caaaaacatc gtttgtcata tcaaatccca aggtacgcag gtatttgatg
      721 aaaaataccg ccgttgttcg ggtgttgcct tcaccaaaca catgtatctg ccataacctt
      781 gaaatacata cagcgagatg atggatgatc tcatccatcg aaagattctt ataggagaac
      841 gttctctctt cggaaatctc atagtccagc gtagccccaa gctccgtggc actgccgtac
      901 agcaccgtcg cgccatcaag cacccattcc tttttcgtga tggcatggtc ccggatacgt
      961 cccgcatggg aatagatacc ggtaaacagc ttcctatgga tggatagata ctcattcggt
     1021 gtaaagctga aggcacgctc ggacagaagc tccgcgattc tggcagcaac cttatcggcc
     1081 tcctctgtgc gatcttccgc attacgggag ggattctctt catgataggc ctgtaagaac
     1141 gcgttcgctt cttcaaaaga tatctcgccc tcgatatttc ggagggcggt atgcgttaag
     1201 tattccgaag tcttcagccc gtcgaccgcc tgtaggccaa tggctgtatg ccatgcatat
     1261 cctttattcc tcttatccgg ctcggactct ttgatatact ctttaaaagg atccttattc
     1321 actccatatc acctcgctga tgttctatac gtcatgatcc aatcaccttt gaacattcca
     1381 tgttcagcga actacatccg gaacacatta tccacaaggc cggcatccat catcacgccc
     1441 gctcgatgga atcatcggac agcaagaaat ctaaaaccgt cagcaggcga tacccatgtt
     1501 ccaagtttga aaacggtgtc ctgtccatcg tgatgaggat tttcttgtat ccgtcatcca
     1561 atccgtgaaa ggatgataat tcccgctcct tcacgcttgg attctctatg ctgaaactga
     1621 cttggatgta atttcttcgc ccctccatat ccctggcaat gaaatcgatt tccttgtccc
     1681 tgtttttgcc gatatcaact gcatatccgc gtctcaacag ctccacatag atgatatttt
     1741 caatggcgcg ggatagttca agctgacggt acagaatcag tgcattgcgg agacccatat
     1801 cgcacacata gtacttttgc cgggtcttga gatatacctt tcccttgata tcataacgat
     1861 ccgctttata gaaaagaaat gcatccgtga catgttgaag gtatcgatca acggtttcgt
     1921 tatcgaccgt cttatacccg ttgcttttca aaatattcgc tatgttgttt gccgataccg
     1981 cagaaccgat cgaggaacac agcaaccgga tcacgccacg gaagagactc tcattacgga
     2041 taccatagcg atcaatcatg tcccgagtga ttacggtgtt ggctaccatg tccaaataat
     2101 cccgtttgtt tgttgtatcc ggctctgtga ccgtatatgg aagggatcca aagaacatat
     2161 attcatcgag ggattctcgt gtatcgccac cgcgaaacga atgaaattcc tgaaaggaaa
     2221 gggggaaaca ctttatttcc atacctcttc cccgaaaaat cgtattgatg tcgctggaca
     2281 ggagcttgga attcgaaccg gtaacatata tatcggtatt gtattcgctg cgaatttcat
     2341 tcagaacttc ctcaaaaccg tcgaccattt gaatctcatc caaaaacaga tagtatttgt
     2401 cgtcatctgc aaattgactg agaagatatg aatagagatg accggaatca cgcaaatgtt
     2461 cccatttctt catatcgaag gctattttca ggatatggtc ggcttgtact ccatcgtcga
     2521 tgagatagtg gtaaaacaat tcgttcaaaa ggacggattt gccggaacgt ctgactcctg
     2581 tgatgatttt aaccaaatcc ttgtccctat atcggacaag tgcatccaat agtctttttc
     2641 tttgaatcag catgacacca cctctcggtg acatcctaaa cgcaaaactt ttattttcaa
     2701 agagttttgc gtttagatac ccgtatttca gccgtccttt tcgagttgtc gcttagtcgg
     2761 catcaccttt ggacaggatc gccaccgctt cgtggtggaa cgtttgagga tagaaatcga
     2821 actgcttgac gctgtccaga gtgaacccga ggtcgaccag cgtccccaca tcccgggcaa
     2881 gggtcaccga gttgcaggag acgtagatga ttctccgggt tccccagccg gcaatcttct
     2941 catgaaggtt cctgtcgaag ccgacccggg gcgggtcgac gatgaccgtg tcgatcctgc
     3001 ccttctggac ggaagcgagg tttgccacgt tcccggtgta gaagtcggag tgcggagcgt
     3061 tcttctttgc gaacgccaga cagcgcttgc ttcgctcgac ggcgatgacc cgcttcgctt
     3121 cgacgaacgc gctgaacgtt cccaccccgc tgtagagatc gatgaccgta tcgccgacca
     3181 cgtgtgcttg aacatagctt acaagcggtt ccagaagaac ttcgttggat tggaagaaca
     3241 cctcgctgga ggcgtacagc gtcctgtcca agacgggaag agcgatctcc cggcccagaa
     3301 gactaatctg ctccccggaa gcgagcgcac cgacctccac caagtgattc cgctgctgca
     3361 gccggtaggc actttcgatc aggagatccg gctccttcag aagggaatcc aatttcggcg
     3421 tcaatactgg acaggaatcg atcggaacca gctcgttgct gttccttccg aggaatccgg
     3481 ccttcctgct tctcaggtcg acatggaagc gcgagcgaca gcggtactcc cagggctttc
     3541 ccacctcggt gggaagcagg ttgatctcat caatcttccc gattcgggtg aagttctccc
     3601 ggatgatccc ttctttgatc cgaatctgct cggcgtggat tgcatattgg aaatcgcacc
     3661 cgccgcacac tccccagtat ggacacctct ccccgatccg ggcgggactc ggttccagga
     3721 tctcgaggag ctcggcgcgg ctgaacccgc tcttggattc aacaagagcg acttcaacca
     3781 gttcgccggg tagaacggct ggaacgaaga ttcgccgccc atcctccggc acggcgagcc
     3841 cttcaccgcc ttggatcact ttctcaatgc ggactcgcac tcttgaactc ctccacgtag
     3901 cgggcgatca cctcgatccc ttcctgccag aaggagcgat cgccaagatc gattcccgct
     3961 tctaggccga catcccggac gctcttcatc ccgctgaccg agagcagggc atcatactgc
     4021 tcgacaaacg cggagggatc gtcctcgctt cgtttgctga gtcccagacc gaagagctgg
     4081 ccgaatgcgt acggatagtt gtagtgggag aactccgggc gatagtagtg gctcttgacc
     4141 gcccacatgt acggatgata gacgtcaagt gccggaccat aggtgcgctt ctgggcatca
     4201 agcatcatct cgttcaactc gcttgcggtg atgtccccct ccttccggcg cttgaacacc
     4261 tcctcctcga agtagaagcg ggaaaggatg tccagacata cctgcgtcga gtcctgaagg
     4321 aacgcttcga tgagggcgat ccgctcctct tcatccgcct cctccaacgc gccccggaag
     4381 atcaggcctt cacagaagat cgacgcggtc tcggcaagcg gcatcggata gtttgcaagc
     4441 agtccgccgc gctgcatcac cttcgagtcg tggtaggcat gtcccagctc atgggcgatg
     4501 gtcgatacgc cactgtaggt gtaatcgaag tttgaaagga tcctcgattc cttcgccacc
     4561 gggaaggagg tgtcgtacgc accgccgact ttgcctttcc gtggttcggg gtcgatccag
     4621 ttcttttcaa aagcgttttg agcgaacttc cccatcgctt cgctgaaccg cgagaagtgc
     4681 tcgacgatga aggcttgggc gtcctcgtag gagtagcgct tcgcgctctt gcccaccggg
     4741 gcgaagagat cgtaaaaggc gaggttttca agcccgagga gctccgcctt tttcttcaag
     4801 tacgaccgga aaagaggtac cgcttcttca agcgtggcga tcagggcgga aaggatctcc
     4861 gagtcgattc gcgattgaaa gatcgaacgc tccagcgggg atccccatcc tctgcgggcg
     4921 ttgagggaaa tggtcgctcc tttgactccg ttcaaggccg cggcgaacgc ttcttcgtgg
     4981 atttccagca gctccagctc cttttcaaac gccctctttc ggatcgcccg gtcaccgtcg
     5041 gtggccatgt tgcgcaactc gacgaccgtc ttcatccgtc cgtcttccca ctccgccgag
     5101 atcgaagatc cgagactctg ctgcaatcgg gaaaaggcgt cccagctgct tctggccaag
     5161 tcggcggcga actcctccaa ttcatcgctc atcagatgct tctgttcgat caggagacct
     5221 tgaagcacat acgcatacgg atcatccgct ccccgatctt tgatttcatc ctcccgcttc
     5281 gccagatgtc gcatgaaccg gacggaaagg ggcttgagaa ccagaacaag atcctcgacc
     5341 tggttgaatg ccttgagata tacggcgttc gtcgtgtcgg tgctcaccag cgcatgggca
     5401 tacgatccca agttccccat atggtccaag atcagctcaa acgcttctat gttgatctga
     5461 aggtcgctct tctcatcggc gaggcgggct tccaattcgg cgcttttggt tcggaccagc
     5521 tcgatgtcgg caagaaactc ggcactctgc ggcccgggat agatgggagt aagatcccaa
     5581 cggggtagtg atttcatatc attctcctac agtgaaagat tatattcggc tattttattg
     5641 atcaatgtcc gacggctgat gccaagctct tcggcggccc gggttcgatt tccctcccaa
     5701 cggtgaaggg cgtggacgat cgcttcgatc tccgcctcct taagggattt cgcctcgctt
     5761 tgaggaagtt cctgaggctg ctgcgcctga ctgtgcagac tcaccgaatg gcgcagatcc
     5821 agatcgcttt cctggatctc cgcctcggtg gcgaagatca tcgcccgttc caggatgttt
     5881 tcaagctccc ggacatttcc gtaaaagtca tgctcagtga ttcgattcaa cgcatcggct
     5941 gaaatgccgg tgacggtgtg ccccatttcg cgattgagcc gtttgaggat gccggcggcc
     6001 agcagcggga tatcctccct tcgctgccga agcggcggaa tgtggatccg cacgacgttg
     6061 aggcggtaaa acaggtcttc cctgaagctt ccgtttcgaa cccgctcttc aagatccttg
     6121 ttggtcgcgg cgatgatccg ggcgttgatc ggcagctccg tggttcctcc gagccggatg
     6181 atcttccgct cctggagaac ccgtagaatc ttcacctgaa gcgagagggg catgtctccg
     6241 atctcatcca gaaaaagcgt cccgccgccg gcaagctcga acatcccgat cttgcggctc
     6301 accgctccgg tgaacgcccc cttctcatac ccgaacagtt cgctctcgat gaggttctcc
     6361 ggtacgcctc cgatattgat cgcgacgaaa ggcccatcgg cgacggggct ggtctggtgg
     6421 atccttcggg cgacaacctc ctttcccgta ccgctttccc cggtgatgag caccatcgag
     6481 gtggtatcgg cgatcttctc gatgaccttc ttgatctgtc tgatttgcgg cgactcgccg
     6541 atgaaaagct cgccctcgct gtccgcctgg cggctttcgc tctccacgac gttgcggagg
     6601 ttctgaagct cgaccagttt tttcaggcga agaaccagct cctcggggtc gaacggcttg
     6661 acgatgtagt cttgggcgcc ctccttgagg gcggtgacgg catcgctgat ctccccgtgg
     6721 gcgctgatca tgatgatcgg catgcgcagc ccctcgcttc gaatccattg gatcagttcc
     6781 agcccgttca tgccgggcat tctcagatcg atgatgcacg cgtcgaagcc ctgctcccgg
     6841 agcatccgtt gggcgctgag gccgttttcc gcctcgctgc tttgaattcc ttcaagcttg
     6901 aggtagcggc tcatcagttg gcggatattg atctcatcat cgacgatcag gacgttcatc
     6961 cttcactctc cttatgtttt ctgagcggac ggatcgcttc gggaagaatc acctccacga
     7021 tcgtgccgcc gccttcccgc cccctcaggc tgatcgaccc ccctcgggcg cggacgaatt
     7081 gctgactgat cgaaagaccg attcccgatc cctggatctt agtggtataa aaaggatcga
     7141 agatcttttt catgtcgctc ttcttgattc cgactccccg gtctttcacg gtgatatgaa
     7201 ttacatgttt tttaccggtg gaaacggcga cttcaacctg agcatcctcg ccttcattgg
     7261 attctaccgc atttttgagg atgttttcaa agattgaacg catccggtcc ggatcgatca
     7321 ggatcgaaag cggccgctcg ctggtatagg cgatcggcat atcgaaggct ttgatgagca
     7381 tctcgaagaa ctcatcgacg ttgacaacct ccggctgccc cagagggttg cggaggaaat
     7441 cactgatttt gttcgtcaat tgggtgaggc ggttcacttc cccttcgatg acctgcaggt
     7501 cctctttgtg ctcctccgag agcgttttct taagcaacgc cacctgaatc gtaaccgcac
     7561 tgagcggatt cttgatctca tgagccagcg tccgggcggc ctgccccaag ttcacgaggc
     7621 tttcctgctt ggccaatgtt tgccggtagg agcggttttt cagatagatc gtcaggacgg
     7681 cgatgaacaa gccgatgatg acgaagcctg aaaagaaacc gagaaccaca atgaccatca
     7741 accgatgatg gtaggcggac ccgtcgagac tcagatagag aagatcggga aagtcgatcg
     7801 gagtcggaag cagcccctgt tcggtgaggg tgagttgacc ggtctccaag agaatcgtca
     7861 accggctgaa ccgaatatac tcaaccatct gggttgcttt gttgtacgtc gcccgtccgg
     7921 tgcggacaaa cccgtcgcgg gtggtgaaga gctcggcggg gattgtcatc ggcaccttgc
     7981 ccaagctcat cactttgcgc cccatcgagg agtagacccc taccccgctt acgttttctt
     8041 ccgccatcgt tcgcagcgct ttggcattgc tgtcctgcag cgcgagaaac acggagttaa
     8101 acgcccgctc cgcctcgttt tgaaggcgaa gttcttcccg ctcatacagc gcggaggtca
     8161 agaaaacaag caatccaaga agtaaaagaa agccgaaaat cagtgatgag ataacgaaga
     8221 ctccttcgcg tccatcgcct atcatcaatc gctttctgct tcgtcgcaaa gtatgctcct
     8281 attcatagtt caaggcatcg atcgggtcga gtttggaagc tttcactgcc ggataccagc
     8341 cgaaaaacac cccgacgaac atcgagaatc ctagggcaag tatgaaggag gcgtacgata
     8401 aatgcaacga ccagtccatc aaattggtta cggcgaagct gagcagcgat cccaacacga
     8461 tccctaaaaa cccgccaaga atcgtcagcg tcaacgcctc gatgaggaac tgtccccgga
     8521 tcaccgacgg gcttgctccg atcgctttgc gcaccccgat ctcccgggtt cgttcggcga
     8581 cggaaacgag catgatgttc atgattccga ttcccccgac cagaagactg atcgccgcaa
     8641 tgagggcgag gaaggtgctg aacgtcccgg tgatctggtc ggccatctcc gcaagggttg
     8701 ccggactgaa aagcatatac ccgtccgttc ccacgatgtc atcgaggtac tcggtgatcc
     8761 tgtcgctgac ggcgatcgta tcgaacccat cggtgacctt cacgacgaat gcaccgacgt
     8821 tcgtcgtccg gatgaagcgc tggctgtagg tgttgtaggg gatgaaaacg ctgtcgtcgt
     8881 aggaaagatt gaacgtcgga tccttgctct tcagaatgcc gacgaccaga tagttcttcg
     8941 cttggtttct aaaaaggctg atatatcggc ccaccgcttc ctgctcggga aagagttcct
     9001 cggcgacgct gctgccgagc accgccacct ggcggcgatt gatgttgtcc atcgcatcga
     9061 agaatgatcc ctgggtgtat tcaagattca gcgcatcccc ataatcggaa agcacgccga
     9121 cgaccgaagc gtcggtggcc tcctgactca cccgtattcg ggcgatcgaa gagttctggg
     9181 gcaggacgac atcgattcct tccacatccc gccggagcat cgaggagaac tcttcgttga
     9241 agattcccgt attccgttgt ccgggtgacg gataaatcgt caccatatcc aggccgccga
     9301 tggcgatcgc atccttgatc gattgggagg cgctgtcccc gatcgtgagg atggcgacca
     9361 ccgatgctac tccgatgacg attccgagca gggataaggc ggtcctcatc ttgttcccgc
     9421 gcattgcatt gaaggcgagc ttgatatttt ctatgaccat ctcacacctc gattttcccg
     9481 tcgcgaagat agatctgacg cttcgccttc agacccagtt cacggtcgtg ggtcaccaga
     9541 atcaccgtcc gcccttcgct gttgatctca tcaaagagct ccatgatctg attccccgtt
     9601 ctcgtatcaa gcgccccggt cggttcgtcc gccagaagga tcgaggggtt gttcacgagc
     9661 gcccgggcga tggcgacgcg ctgcttctgt cccccgctga gctcattcgg ccggtgatac
     9721 atccgatcgg cgaggcctac gctctcaagc gcgttgatcg ccatccttct ccgttcacgc
     9781 ggcctgtagc cggcatagag caacggggtg accacgttct ccagggcggt gagcttccca
     9841 aggaggttga actgctggaa cacgaagccc accttctggt tccggatgaa ggcaagctcc
     9901 gcttcgttca tcccgctggt atcctctccg tcgatcagga tctggccgtc ggtgggcgtg
     9961 tcaagacagc cgatgaggtt catcatcgtc gacttgccgc tccccgaagg acccatgatc
    10021 gaggtgaact cccctttttt gattgtaatg gataccccgt ccaacgcttt gacgatgaat
    10081 tcgcccatca cgtagtagcg gctcacgttc cgaagggcta tcacatcact catcggggat
    10141 ctccaccatc atgttcatgt tgccgcccat cgaggcgaac ggattgctcg tatcggttcg
    10201 gcgggtgacc accgtgtccc cggctttgag atctccgcta agcagctggc tgatcccctc
    10261 gccgaggtac ttcaccgtca ccggaacccg ggtcgtggtt ccatcctcgt tccgcttcaa
    10321 gacggaggaa ctgccccgcg aggtggtcac ggcgctttga ggcaaaagga tcagggcggt
    10381 ctccccttcg ctggtgatcg ttcccttgaa cgtatacccg ggaacaatcc gggcgggcgg
    10441 actttcaatc gtcagctcga catccatcac accgattccc tgattggtat accggccgag
    10501 catcggaatg tagctgacat atccttcgat ggaaccgtcg gtgtaggaat cgaagtaaag
    10561 aacggctttc tggccgagtt cgacatattg catatcgatt tcgtcgattt cgacggtcgc
    10621 cttcaacgtc gaccggtcga cgatcgtcat cgccgtctgt ccggcggtga agtagtcccc
    10681 ttctgagacc ttcaccgaag cgacgacacc gtcgaagttg gcgtaggctt tggtgtaatc
    10741 cagcttgttc ttggcgttgg tcagccgaag ttccgccaac tcgatctccc ggtcacttgc
    10801 accggtgagg atcgccgcct gtagggcatc ctggctttgc gctacatcga agcgctcatt
    10861 gatgtcatcc aaggcgatca gcacatcgcc cttcctcacg ctgtcccctt ccttcacgta
    10921 cacgtccgta acggttccgg tcgctcggag aatcacctgc tggatatcgt tgggctcgac
    10981 gaaggaggag agatcgatcg tctgggtgaa gatcgattca tagacctcgg agtccgtttc
    11041 ccggacgaaa ctgatcggag cccgctcttc cgcccagggg aatcggttgt tctccttgta
    11101 aaaagtgtag aggtagagcc cgccggcgat cagggcgaga atgaagatcc aggtgaagaa
    11161 gcggcgggct ctccgcctcc tttgtttctt cttgaattgg ttcttcacga gggtttccgt
    11221 tgcagtatcg cttctctgca tctcaaacat gctctttctc cttacatcat caagcgcttg
    11281 atttcatttt caagctgcaa gcctttcagc agcatcgctt tcaggtcgta ttcatcaagt
    11341 tcaacggtaa aagcggcgtc ttccacctgt ttcttcgtca caaggccgtt ctcaaagagt
    11401 tcccgctgct gcttcagcag atccttgttg taccggatcg tctgcaagag gagcgcatac
    11461 tccaactgcc aggaggcgat gccgttctgc acctcgatcg agctttggat gtactcatcc
    11521 acggcattat tatatgcgat ctcggtcagc agcacctcgt tctcaagctg ccggatctcc
    11581 agtgtatcct tggcgaaggc ggggtcgttg gaccaggttc cggagatggc gaccacgggc
    11641 gtcagggtcc ccttgtcgaa atcgtaggtt ccgccaaccg ccccttggac ggtgaagctc
    11701 ttgccggcca gcgcggccga acctcccaag gtgagactgt tttgtgtcat cggaccaagg
    11761 ttggtattga tctggctgtt ggcgtaggag atgttcccgc ctgccaccag cgtcctgttg
    11821 gtgtattggg cgatccggag ggcgagggtc tccttcgcct tttgaacctc gatcctcttc
    11881 gaagcgacgg aggtcgagcc ttccgcattc atcgcaaagg aaagatcggg ttcccggatg
    11941 tcggtgatcg catcgggata cgggacgccc atgatggttt caaactggcg gatcagcaga
    12001 tcccgggatg cctgaaggct ctcgagggtt ccttccgtca atcggatcgc ctgatcctga
    12061 gcctgataga cgagactgcc ctttttgacc tgccccagaa ccagtttgtc actgagttct
    12121 ttttttgcat cggtaagctt ctgcgtagtg ctcttgatcg acttctcatt gtttacgagc
    12181 gcgccgatct ggctgtagag ggtgttggcg aaggtgagct tcgctgattc atagctcgaa
    12241 tttgccatca tctccgtctg ccgggtgttc agatccttca gcgaatcctg agtcttgcca
    12301 tacgtaatgg tgtggctcat cgagagggag gggtcttcaa gatagttcca cgaagaaccg
    12361 gcctggctgt aggagaaggg atcgacggaa aaagagaacg cagtcttccc gtcgttcggc
    12421 agcgtaacct ccacgctgct gccggaaagc gagatccgct gacgggagac gttccagttg
    12481 ttcttcgtgt cgatcgggtt ggaatacttc aattctccgg agttcacgct caccttcacc
    12541 ccgtcatcga tcttcgtcgc cgcctgctgc agaaggacgt tgctccgggt gatctcgagg
    12601 tttttcatcg aagaggagtt ctgctcggct tgtttgagca ggtcatcgag gggtatcgcg
    12661 gaaagcggga gggcggcaag aagcagtact gcggcgacta ctcccacccg tctcgtatat
    12721 ggtcggtgca tcaatctaca ctcctatcgt tacatactat caagtatcgt gatctttcgg
    12781 ctcttcggca acctgtgagg ggcttcttca cacaaactac aaaagttttt gccccccgtc
    12841 gaccgccagt tcaataccgg aaacgaaatc cgcttcgaga agatacaaag ccgcctcggc
    12901 gaccgcttcc gccgtaccgg ttctcctgag cggaaggatc tgctgcattg ttttgaagta
    12961 tgcttcatcc ccgttggaga gaaccgctcc cggactgacg gcattgaccc ggagggtcgg
    13021 cccgagggca acggccagca ccttcgtctg ctccagcagg gcgctctttg aaaggtaata
    13081 cccgactcgg gaggcggtgg gcttggagac ccgcgtatcg acgatgttga tgatcgagcc
    13141 ggtccggccg cgctcaagca ggtgatgata gagcgctttg gacaagaaca gcggagcggt
    13201 tgtgtggagg ttcatcatcc gctgatacgc ttccagggtg agctccccga tcgtccccct
    13261 ctcaaaacag gaagcgttgt tgatcacatg gtcgatcact ccatatgcgg tcaaggcttc
    13321 ccggaaaagc ggctcgaccg cctcggggat cgaaagatct ccttggatga tctttgcccc
    13381 atggattctc tgcaattcga aggcggccgc ttcgcttgtg ttgtaatgga cgacgacccg
    13441 atagcctttt tggagaaggc tttgggccat gacgcttccg aggcgtcggg ctcccccggt
    13501 tatcaggaca acttcgctca tcgacgaaaa gtataacggt gttgttaccg aatgacaatc
    13561 gagggtttat atttcagata aggtggacat ctccaccaaa ggacatgcaa tgagcaatga
    13621 acacaaaaac gacaaggatt ggaaagaatc cttgaagaag cggatgaaca tcgagccggg
    13681 catccatacc ggcgacaaag gtccgcaaaa gaagtatact ttttcattct ggtatttctt
    13741 catcatcctg atggcgtttc ttctggtgaa ctccttcatg gtaagccgcc agcccaacat
    13801 cgtccagatc gactacaacc agttcaagca gctggtggag aaccggacga tcaagcgggt
    13861 gaacatcgaa acggagcgct atgtcggcta ctccttcagc ctgggccaac tcgccaccga
    13921 cggcatcatt cccgatggag gagcgcaggc gttcgccacc tacaaagtgg aagacccctc
    13981 gttcgtcccc ctgcttgacg agatgggcgt ggagtacttc gccacgccac cgccgaaacc
    14041 gagcatcttc agttcgatcc tctcctatct gctacccttc ttcttcatcg tcattctctg
    14101 gcgcatcatc ttctcgagga tgggcagcgg aggccaggga gtcctctcct ttacgcaaaa
    14161 caaagcgaaa atcgtagccg aaggggacac cgggattcgc tttgcggatg tggccggagt
    14221 ggatgaatcg aagtatgaac tggaggaagt cgtcgacttt ctgaaaaacc ccgacaaata
    14281 caccgagatc gggggaaaga ttcccaaggg cgtcctcctc gtcgggcctc cgggaaccgg
    14341 caagaccctt ctggccaaag cggtcgccgg tgaagcgcag gtgccgttct tccgaatgag
    14401 cggagcggac ttcgtcgaga tgtttgtcgg agtcggagcc gcccgagtcc gggacctctt
    14461 cagacaggcc agagagaact ccccctgcat catcttcatc gatgagattg atgcgattgg
    14521 ccgcgcccgg gtcggagcgg gcatcggggg caacgatgag cgcgagcaga ccctcaacca
    14581 gcttcttgtt gaaatggacg ggttcgactc ccggaccgga gtcatcatcc ttgcggcgac
    14641 gaaccggccg gaaatccttg atcaggcgct cctgaggccc ggtcgattcg accggcaggt
    14701 tctggtcgac aagccggatt tgaagggccg atatgagatt ctgaagatcc atacccgcaa
    14761 gatcaccctc ggagacgacg tcgatctcaa taagatcgcc caagcctcag cgggcctcgc
    14821 cggcgcggat ctggcgaaca tcgccaacga ggccgccctg atggcggtcc ggggaggaag
    14881 gaaagcggtc agccaggcgg acttcgagga agcgatcgag aaatcggtcg ccggtcttga
    14941 gcggaagagc cggatcctca atcccaagga gcgtgagcgc gtcgcctacc atgaaaccgg
    15001 acacgccctg accgcctatc tgacggaagg ggccgaaccg gtgagcaaga tttccatcat
    15061 cccccgcggc ttcggagccc ttggctatac gctgcagtat cccaccgaag atcgcttcct
    15121 cttaagcgaa agcgagctga tgatcaacat cgacaccctg cttggcggcc gggctgcgga
    15181 agaagtcgtc ttcggggaca tctccaccgg agcgggtaac gacatcagcc gagccagcga
    15241 tctggtcagg cggatgatca ccgagttcgg aatgagcgac cggtatcgga acatcacgtt
    15301 gccgaccacc cagagcggca tcgccggggt cggtggaagc cgcgactact cggagaagac
    15361 ccaggagtac atcgacaacg agaccgccag aatcgtcaat gagcgctacg aaatcgtaaa
    15421 agccaacctt gaaaagaata aggaagcgct tttgacgatc acttccgagc tcttggagga
    15481 tgaagtctta agtggagacc gctttgaaga gcttgcaaaa agtaaagtca ttggctaatc
    15541 tctagctcat gctctataca ccgatcaccg accctgaact gacccgacac ctcgccaccc
    15601 ttcccaaaga cggacgcgag gtgttcgtgg ctttggatgc aaccgtccga atcaccgcga
    15661 tcagtgcgac gacgatgatc aaccaaatgc aggccaacca cggtcttcag ccgctggaaa
    15721 cgtacgtcct cgggcaggca taccttgcca ctgcgctcct tacctcactg gtggaaggaa
    15781 atgaccggat gcagctctcc atcgaatgcg gaggcccgat cggcggaatc tacaccgaag
    15841 gatgggcgaa cggttcggtt cggggatacc tgaagaacaa cccgattccc ctgacgcggg
    15901 aactgacctc tctggatttg gatgaactct acggtccggg attcttgacg atcagcaagc
    15961 tcttggaggg gaagaagact cctttcagcg gtcagacgat gcttcagtgg ggtgacttgg
    16021 cgaaggatct ggccacccac ttcgaccaaa gcgagcagac gcccacccac ttcatcctct
    16081 ccatcgattt caacgacagc gcccaagtca tcggcgcggg agctctcttc atccaggcga
    16141 tgcccggctg gaaacaggat gggttggatc ggcttgccaa ctgcgccgac gagcttcctt
    16201 ctttggggaa gcacatcgca aacggctact cggtcaaatc gttcgttgaa acccatttca
    16261 ccggtgtaga tcttcgccac ctcggcagta cggatatccg attcttctgc ccgtgcacca
    16321 gggaacagta cggtcggtac atccagcacc tgaaggaaag cgaccgcgcc gacatcgcgg
    16381 aaaacggacc ttttcccttt gaagtggtct gcgcgaactg caataccacc taccggtgga
    16441 gcaaagatga aatgaaacgt ctcctcgatg aggtgaagcg atgatctttt atgcgaccag
    16501 cggcctccat caaagcgata tcctggccga agaagcccgc atggcgggag cggaagagat
    16561 cgccacgtca agcagcggag ttcatttcac cggggatctg cgtgttgctt atcgcttcct
    16621 gcttacgtct cacaccgcaa cgcgcctgct cctcggcatc tatgaggacg atgatatccg
    16681 cagcgccgac gagttctatg acgcgtcgat gatgattccc tgggaggagt ggatcgatcc
    16741 ttcaaaaagc ttttcggtga cgattaccac aaagaacgtc cgctacctga agcactcgaa
    16801 cttcggagcg ctccggctca aagatgcgat cgtcgaccgg attcgagaac acttcgacgg
    16861 ccgaagaccg aatgtcgacc gtgatgacgg cgacctcatc ttccacgtcc acttggaaga
    16921 agatcgggcg acgtggttcg tcgatctcag cggtcaaggc caccacaagc gaggataccg
    16981 gggggatcag accgatgctc ttcttgctga acacctcgcc ttttccctcc tctatcgctc
    17041 ccagtggcgc aagcttgccg acgaaacggg ctcctttccc accctgctcg accccttctg
    17101 cggctcggga acgatcgtca ttgaagctgc gctctgggcg accgggcgtg ctcccggcct
    17161 cgtgaaagcc cggatctttc ccgttttcaa cctcccgatc catgacgtgg atctctatga
    17221 agagatcgtt gacgaggtcg tctcccggat ggaagaggaa tcgctttcca tccatggatg
    17281 ggaccgcagc cccaaggcga tccgcatcgc agaggaagcg gcaagagaag ccggcgtcga
    17341 tcatctgatc accttcgagg tgaaggattt caccaccctg acggcagaag acattccccc
    17401 ttcgaccggg gtaatcgtaa ccgacccccc gtacgggatg cggatgggcg agtgggagga
    17461 gacccgccac ctgtatcgct cgatcgggga tgccctcgcc caattcttcg gcggatggaa
    17521 cgtgagtctc ctcaccgccg acgatcgcct cctggggcag atcgatctga agccgaatag
    17581 aaccaatgcg ttcaacaacg gaggaatccc gtgtaccctt gcccactact acatcttcac
    17641 ccatgaagag aaagcggctc tgagggcgga gtgggaacag cggagaaaag aacggctcag
    17701 ccagccgctt tcaagcggtg ctcagatggc gtacaaccgg ctgatcaaga acctcaaaga
    17761 gctgaagccg gtcatggaac aggagggggt gacgagctat cgcatctatg atgcggatat
    17821 gatcgaatac aacgcggcga tcgacctcta cgaaggaaag tgggtcaacc tcgccgagta
    17881 cgccgcccct ccgaccgtcg acgtcgaaga cgccgaaagg cgcctcaatg agttgatcga
    17941 tgccaccgaa cgggcaaccg gagtagaccg cgagtctatc tttgtaaagc aacgaacggt
    18001 tcagaaaggg ctcggccagt atgagcgcca ggcatacacc gaccggttct acatcatcaa
    18061 tgaaaacaac gcccgctact tcgtgaactt caccgactat ctggatacgg ggatcttcct
    18121 cgaccaccgc ccgatccgaa aatacatcag cgagattacc gaccataaac gctttctcaa
    18181 cctcttctgc tatacgggga ccgctacggt tcaagcggcg aagggaggag ccctctccac
    18241 cgtcagcgtc gacacctcaa cgacctatct ggattgggcg cggcgaaaca tggagctgaa
    18301 cggcttcggc ggaatgaacc acttctacta tcaaagcgat tcgatttcat tcctctggga
    18361 tacgttcgac cgctacgatg tgatcttctg cgatccgccg acctattcca actcgaaaga
    18421 ccgggcgccc ttcgaggttc aaaaggatca tcgctcgctg atcaaggcgt gcatgatgca
    18481 cctcgatccg ggcggactgc tcatcttcag ctgcaactat cgatcattca ggatggatga
    18541 ggaggtatat gatgaattcg acgtcgagga gatcacccct cagacgatcg gaaaggattt
    18601 cagccgcgat ccgaagatcc accatgcgtt cctcatccgg gagcgggtga aaatagtggt
    18661 gcaaccacaa aagccaaaga gagtgattcg acgaaagccg gtcgactgaa aaaggggcct
    18721 cgaaagggcc ccttcacatg atcaaaatat cacttagaag atatatccga acgacatggt
    18781 ggcttcgagg aactggtgtc cgccactagc gaacttgtcc agatcgacgc cgtactttcc
    18841 gccgattcca agggtgaagt ggtcggtcag gcggtaggaa agcgccgcct tggcgaacac
    18901 tccatagcgg taggtgagcg tgctgctgtc gatgaacgaa ggatcaaggc tgtaggttaa
    18961 gaagccgcca cctccgagac cgagggctat gcggccgcct tcgacgttca gcatcacggc
    19021 gggactggcg gtgaatgagt gttccagact cgtgaagaag gtggaaagcg cgtcgaagtt
    19081 ccctttgaga accttgctga gggtggctcg gtaggttgtt cccctgaggt acggggtgta
    19141 ctcgagatca aggtcgacgc cgagtccgaa cacctttgcg aaggtaagga tgttgtggaa
    19201 ctccaacgtc ataccgccct tcaggttgtt gatatcgtag gcggagttga accgggaacg
    19261 ctggcgatta taccccagat tgattcccag cttcgtgtag aagcggctcg gctccttcac
    19321 cttcggggcc ttgggcgggg tggcaaccgg ttctgccttc ttgacctctt gcacaggtgc
    19381 tgccggttga ggcgctgagg gagcgggtgt gaccggcttg ggtgcaacgg gctcctcgac
    19441 aacggcttgc ggctccggtg caagaatcat tgcctcggct accgatcggg tgctctcgct
    19501 gaagtggatc ccgtcatagg actgctgcag gtagagcgta tggtcgacgg ttccatccaa
    19561 tcctcgttcg gtgtagctcg tgacgcttga atcgacggtg atccagcctt ccggtttctc
    19621 cgaatctatc tggaatcgga aggttgtgac ttccggatct tccaacaacc attcccacgt
    19681 tattacgacg gggacggcaa acaacatggc gggaagtagg atcaagattg ccaacaacgc
    19741 tgtgaatttc aacttcatgg tttataactc ctatctgtaa cctacgacgg actacacgct
    19801 ttgatcgtag ttcagtacta taataataca cgcagctcat taatataaca agtaaatagg
    19861 cgaaatcgat acagaatagg agaattttac gagatgcgcg actcaattca aacgcttgcg
    19921 gtgttttgcg gctcgtcaag cggacacaac ccgctataca ccgaggcggc aaaggcgatc
    19981 gctgaaacga tggccgataa caacatcgct ctcgtatatg gtggaggtaa tcgcgggttg
    20041 atgggcatcg ttgccgaaac ggttcatcga cgaggcggtt atgtgatcgg ggtcattcct
    20101 cagttgctca accgagcgga tgtcgtgatg cgcaaggtcg aaaacgagct catcgtcgcc
    20161 catgacatgc atgaacggaa agcattgatg tatgagcgga gcgatggatt catcgccctg
    20221 cccggaggaa tcgggacctt ggaagaactt cttgaggtct atacctggct ccagctcgga
    20281 tatcataaaa aaccggtagc cgtattgaat atcgggggct tcttcgacca cctgcttgat
    20341 ttgctgactc acgccaatgg cgaaggattc atcaaagatg acatcatgaa ctcgctgatc
    20401 gtcgctgacg acattccctc cctttttgaa cagctcgatc ggtgggacgc tcagctggga
    20461 gacaagcttt caccatgaag atcaatcgct acctcatcat cgaatatttc aacgtcatag
    20521 cgggatcata cctcaccgca ttgggaatcg ccctgttcag tgcgccaagc aaaatcgtga
    20581 acggcggagt cagtggaatc gccatcatcg ttcaccacag cctcgggtgg gataccgggt
    20641 tctccatttt cctcttttca atccccctgt ttctcctcgg gcttctcgtc tttggcaagc
    20701 gctatgcggt aaagagcctt gcgggaacaa tcctcctaag cgtgttcacc acgatgaaca
    20761 acatgatttt cggctacgag ggggttttgg atccttccaa cgtcctctcc gtcctattaa
    20821 actcgctcag cgccggagct ttgatggggt tggggcttgg aatggtggtc cgcagcggat
    20881 cgaacaccgg tggaacggat atccttgccc aggtggtggc ccgctatact cccctttcga
    20941 tgggaacctc ctttctcatc gtcgacggat tgattatcct cgtcggagca ttgatcttcg
    21001 gtcttgagat ggcgctctac gccctcatta ccgtcttcat caccagcgcg gcgatcgaca
    21061 aggtggtcct cgccttcggg cgtcgttcgg caaaaacggt gtttatcatt tcaacgcagt
    21121 tggaagcaat aaaagatgcg atcctcaccg agctcggtca tggcgggacg attctttccg
    21181 gcaccggaat gtatacccgg accgaccgtc cggtgatcat gaccgtcgtg gcgaatacga
    21241 agctcgccgc cttgacgcac atcgtccacc ggtacgataa aaacgccttc atggttgtcc
    21301 aagaggcgtt caatgtgctc ggcgaagggt ttacaccgat cgaggaggga gcatggagtt
    21361 cccacctcga cattacccag gagcagcgtc cttaattgag ccagagctgc tcgggataga
    21421 gtccctcttc agtttttttg gcaagttgaa gtttcacgtt ttcccggtct tcggcgtagg
    21481 tcattccgaa ccaatgctcg tcagtggtgt agaaacgaac ttttccttca ccttttgcta
    21541 cgaggtttcc cactccgttg ggaaggtagc actcgttctt ttcactggtg acgtgctctt
    21601 caatgaaggt gtgccagtag cgttcaaagg aatcgaatgc actgtgggcg aatccgaaga
    21661 agttcatcga gacccattct ttcccggtaa gcggaaggtt cccgctctcg cgaaagctca
    21721 tcagagaatc gccttcatac ccgatcttcg tgtgttcgac catgctcttg aggtacccac
    21781 ccttttccga gcagattgcc cgactgaccg ttccgattcg acttgtggtg ttttcaagga
    21841 catagccgac catggcgtgc tccttggcat cgggggcgag accggctaaa tggttataca
    21901 gagtaacata aggggtttta ccatagtaat cgtcagcgtt gatcaccgca aaactcccat
    21961 gaatctcttc tcgggcacag agcaccgcgt gaatcgttcc ccacggtttg acccgtccca
    22021 ccgagagttc aagctgctcc tcattgagca gggaatcctg atgttggaat acgtaggttg
    22081 catccatgtt tctggcgatt cgatcgaaaa ggtggtagcg aaactctttt tcaatggact
    22141 tcctgactac aaaaacaact ttttcaaacc cggcctcttt ggcatcgaag atcccataat
    22201 ccaggagggt ctgcccatag gcgccgacac tatcgatctg cttgatgccg ccatagcggc
    22261 tacccatccc agcagctaaa atcagaagcg ttggtttcat cgtcttcctc cttggtaacc
    22321 aaatcgcgcc tattctaccc tctccgctta aaaatagtca aatccttcac ccccgggaaa
    22381 ccatctggta ctgaaggtca aagtggagta tcctctcttc atgaacaatc acgaacgctt
    22441 taccaatgtt ttggaatttt gtgcgctcag cccaacgtgc cgaacctgtc cctactcgtt
    22501 atatacccgc cgtcatcagg atgcttccaa caaggcgtgt tttacatcca aagaaaatct
    22561 ggaagcgctc gaacgatggc tgcacagcga acatgaagag gacgatcctg ccgaagagtt
    22621 cctctccgat gaccaaaaat ggcttcagga tctcctgaag ctccaattca acgatgatga
    22681 ggatgagttt gacttttagt cggtggtcac tgtgaactga atcgctgcac tataggtgcc
    22741 ggttgcgaag cgattgggat cgaaattgtc gatcttggca taaatctctc cgctttcctg
    22801 aactccaaac gagttcgaat cacgatcctg cggtcccacg gatctggaga cgatcgagga
    22861 gccttccacg gcaacggccc gcgcatcacc atagggaata aagcctgcag taaacggaat
    22921 ctccgtttca agatgggggg catggggagt cccgggggtc aaataaagct tgaaccgtgt
    22981 tcccgatcca tcctcgccgg ggcggatttc aatcttgaac cgtgcatttc caacatttga
    23041 aaagagtcga tatcgaccga tgggaacgat gcttccccgc gcttcaatca aatcgacgtt
    23101 ttcagcctcg gtggagacct cgatgcggaa gtactggtct tcgggcattc gtccttggag
    23161 cgtgaatgaa ctgattaccg ggctgttgtt gcccgacaag gacccgagaa cgatcacggt
    23221 gaagataatc gcccatacaa gccgcttcaa ttgctgcatc aaacctctct ccatattctt
    23281 ttcctcagcc acagtgtaca aagcgaagca acggatgtca agagttttca ttgtatatct
    23341 aacatttttt tctgcaaaac agattaatat caaacaaatc ctttatttat aaatagttac
    23401 aatacctatc ccttctgaca tagggttttc attcgccacc aatcatgtgt atgcggcgtg
    23461 agaatagaac acgatggcaa cgggaagaga atgatgtatg ccgaatggat accgttgaga
    23521 gctcgccaat caaccgaacg ttggaatcgg attcctgaat ttgagtaaaa gggttgaaca
    23581 tcaccgcgct gattcgttag tattacggct agcatttgcc gagtccgtag ctcagttgga
    23641 tagagcgtct ccctccggag gagaaggccg tgcgttcgaa tcgcgtcgga ctcaaagcag
    23701 ggagactggt cattcagtct ccttttttgc gggcgtatac tttttccgtc cgattcggta
    23761 tcctttgaac catgagtatt cgggtccttt ttttaggaga aatcgtcgga aaggcgggag
    23821 ttcaggccgt tcgtcaaaac ctcagaacgc tccgggaaga acaagcgatc gatctggtga
    23881 tcgcaaacgg ggaaggagcc accggaggat tcggtctcgg caaagcccat tcgatgcagc
    23941 tgctgaagag cggcatcgac gtgctgaccg gaggcgagaa gatctactat aagcttgaca
    24001 tggtcgagtt catcgacaag aaccctgcga tccttcgacc tgcaaacttc ccccagcaga
    24061 accccggtcg gggagttcgc tatctgaacg tcggtgaaac caaaatctgc gtcatcaaca
    24121 tcctgggcaa cgccgatttt ccccgaaccc atttgaccaa tgcgttcccg ctggccgaat
    24181 acctcgtgac gaaagccctt gaagaggggg cgattcccct tgtgcaattt cacgcttccc
    24241 ccactgccga gaagcaggcg atggggtttc atctggcggg caaggcggca gcggtcatcg
    24301 gaacccactc caaggtgctt acttccgatg agcagatcct cgacggggga accgccttca
    24361 tcagcgataa cggacgctgt ggaagccaga tgagcgtcgg gggattcgaa ccggaagccg
    24421 agattgaaaa acagatcacc cagctgccgg ttcgcagcaa agagtactgg gaagacactg
    24481 ctctggttgg agtaatcgtc gagatcgacg agaccggcaa ggcgacggcg atcgagccga
    24541 tccgttctgc cctgaaggag gagtaaccgt gttggaagtg gttaccgttc aagatatccg
    24601 ctcaaaggat cgcatccgag tctcctgctc gtcagcggcg tgccaagggt gcaaaggcgg
    24661 tcttttttgc aatacgaaga aacgggtctt cgatgtctcg aacatcgacg gtctggcgat
    24721 cgagaaaggg gatcgagtgg agattcacct gaaaaccggc cggacgatcg gcgggacgct
    24781 ccttaccctc atcgccccgc tcctgctctt cccgcttggc tactacaccg ccaagagcct
    24841 cggcgccggc gaagggatct gcacgctcat cgccctcggc tcaatcgccc tcggattcgt
    24901 gggggtgtgg atctatttca aggtcaacga gcactcctac cttcccaccg tcaaacgtgt
    24961 catcaaggac gctgaataag attgcccaag ctcacccgga aaggtatact caaaccatga
    25021 tgaagcgaat ccttggcatg gttcttgctt ctctcctgct cctttcctgt gccctggaac
    25081 cgacgatcca aagcccggtc gattcgagca agcgagaagc ggacctcact ctctatgacg
    25141 cgacgtacac cttcggcatg tcgaacggta cccctcttgc cgtaaccgcc ggggtgctcg
    25201 catttcatga agaagaacag gaagctgtgc tggaacacgt caccttcatc ggttacgaag
    25261 aggacgggaa accgtttttc caaggttccg cccggcatgc ggtcatcaac acaaccacct
    25321 acgacgccac cctttcggag aaggtctccc ttcaacacct cgatgaggag atccacgtct
    25381 tcagcgatcg gctttcctgg tctgacaaaa accagcaact gtcctccccg ggggacatcg
    25441 cttcaacgat catcatcgat ggggacatca ctctacgcgg cgaagggttg atgatcgatt
    25501 tcaccaccct ttccgtcacc ttcaagaagg ttcttgaagg gagggtgagc cgatgaaacg
    25561 actctttttc ctcatccttc tcatcgggtt cttccttgct tcggcaagcg gaaacgatat
    25621 cacgttcggc ggggggaaca cctccattca gacgcaagcg ggacgacagg tgatcacctt
    25681 aagccaaggt gctcatattc aaagcggttc gatcatcctc aaagcggaca cgatcaccct
    25741 ctatggcgat ggattccgct acgtctcctg tcagggaggt ctttcgctcg ttgatgaaga
    25801 gcgaaacctg aaattgaaag cgaaccgtct cttttatgat cgagaggcgg aggaaattct
    25861 tattgagggg tatgtcgagc tggacgatcc ggccaatgag ctgatcgccc gagggttcgg
    25921 tcttgaatac cggatcgatg aacagcggat ctcgctgcat gcggaagtaa cgctggaaaa
    25981 acacacctca agcggagcga tggtctgcaa agcggatgtg atgagcttcg atcgaacagc
    26041 gatgagcctg cttctcagcg gtaaagccag cgttcattgg gcgggcgacc gatatgaagc
    26101 gcagcagatt gtgatcgacc tcgaacggga agagatcacc gccgagggtt caatccgggg
    26161 agtgatccat ggttaactcc ctcatcgtct ccaacctttc caaacgatac ggtcgccggc
    26221 tggtcgtaga cgacctctcc ttttcgatgc gctccggaga gatcgtcggt ctcctcggtc
    26281 ccaacggagc gggaaagacc accacgttct acatgattgt gggattcctc aaagtccgaa
    26341 agggttcaat catcctcaat ggggaggatg taacgggtct gccgatgcat gagcgctcaa
    26401 aaaaaggact ttcctatctt ccccaggagc cctcgatttt ccgaaagctt tcggtccggg
    26461 acaatatcct gcttgtcgct caaagtcgga acgatttgac tcgacatgaa aaacaccatc
    26521 ttgtcgacca actggtcgag gagttcggac tgacgcacgt ggtccgccaa aaaggatata
    26581 ccctcagcgg aggcgagcgg cgccgctgtg agatcgcccg cgctcttgcc gccagtccga
    26641 cgtttttgct tctcgacgag ccgttcgccg gcatcgaccc caaggcggtc tatgaaatca
    26701 agctcctgat caaaaagctt gcaagccaga gaatcggcat tctcctaacc gaccacaacg
    26761 tccgcgacac tctcgccgta accaccaacg cccatatcat caatgacggt tcgatcctcg
    26821 tctcgggtac gaaaaacgag ctgctcgccc atgaactggc ccgaaagatc tacttcggcg
    26881 atcagttcga gggggaactc tgatggcgtt gactccttca ttgaccgtaa cccaaaaaca
    26941 acagctcaag ctgactcctc agctgattca atccttcgag ctgatgaccc ttcctctggc
    27001 ggaccttcag cagcggatca aagaggagat cgctacgaac cccgccttgg agattccatc
    27061 cacccgcgaa ctctcctacg aaacggttgc cgacaggaag gtggaaagcg agctccgccg
    27121 ggtcgatgat tcctatggcg atacaagtac ctatggcttt gatgatgacg caaccgaacg
    27181 ggttcaccgg gttctcgagc aagtgctcac cgccgaggag acgttggctg agcacctcat
    27241 cgatcaactg cgccttgagc gcttaaccga agaggagctg gaaatcggtc tgagaatcat
    27301 caccaatctc gactcaaacg gatttttcat ctatgaaccg gagctcttca tcgatgaagc
    27361 tgagcgcgga tcctatgaaa aagtcctaag tcttctccac accttcgatc cggtcggcat
    27421 cgccgttccc tcgtggcggg agtccctgat cgtccaggcg gcctatcaag gccttgaagg
    27481 agagacgctg cagcagttca ccgcgctcgt gatggacaat ctggaattga tgcgcgccaa
    27541 caagctcgag caggtggcga agaaccttgg gatcagcgaa gaggagctcg gtgatctcta
    27601 tgcgtttctg aagaccctga cccccttccc cggccagggg ttcgcctccg gtcctcagca
    27661 ctacgtcatc cccgaccttt caatcagggt tgaagacggg gcgctgcaat tgcgaatgaa
    27721 tgaggatgcg ctgccggata tccggatcaa tcccgagttt gaagcgcttg cctcggaccc
    27781 ggatgcgaag acatatatcc aggaacaggt caagaaagcg aacgatatca tcttccagct
    27841 ggatatccgg aacaaaaccc tctacaaagt cgcgttggtg ctgcttcgtg aacagcggga
    27901 gttcttcatt ctcggtcctc agtatatcaa accgctgacg cagaaagcgg tcgccgcgga
    27961 gatcggggtt catgaaacaa cggtgagccg gatcgccacc gccaagtgga tcgagaccga
    28021 cttcgggatc atcccgctca aacggctctt ttccagcgcg atcggcgatc tctccaaaga
    28081 agccgtcaag gagacgatcc gccagatcct cgaggagcag gagggccaaa aagcgctcag
    28141 cgaccagaag atcgccgacc tgcttgccga acggggcatc aagatcgccc gaagaaccgt
    28201 cgccaagtac cgcaacgagc tgcagatcga tccctcgtac atacgaggaa ccgattaacg
    28261 tttaaggggc gccgaggcgc cccttactta ctcatttccg cggtctatta tccgtgattc
    28321 tttactttgc ttttctcctt tcgagcggtc gcctcgatct tgtccacgag cagttcaatt
    28381 ccttcgtaga gctcgtaaca atcggtgctg accattttga cggtacccca tgaaaagtgg
    28441 atctttgcat cgagatggaa tccctgacct gcagtctcgc gggtcattac gatatccaga
    28501 tcatgcaaga atccttcagc aaactgaagc ttttgaagtt ttttgtccaa gaattcccgg
    28561 gtctcgtcgc ttggattgta ccggatgcct ctgacggtta aattcataag ccctccttag
    28621 tagtcttact ctaagaatac gaccttgaaa gtcaatcgtc aatgaaaagc gagatatcga
    28681 cgggctgaaa cgctcttgct cgccacctgt tttatccctt gccaggtgcc accaacaact
    28741 gtatagtagt atccgtaatg gctgaattta ccgtatttga tctgcttgac ttggatatca
    28801 aggaacgaag ccacctgcgc ctgaggtgcg tagccggccg ttcggggttg tcacgggcga
    28861 ttaccaccag caaaatcagc cgcccgggct tgccgttaag cggttttttt gaggagttca
    28921 gcgcccaggc cgttcaaatc ttcggccgcg gggaacaggc atacattcga atgttggaag
    28981 aaaccgatca gatgggcaat gtggagcaat tcttctccta ccccatccct gcatgcatct
    29041 tcaccgacca tggcgaaccg agcaagcaga tcatcgagct ggcggaaagc tcctccgtcc
    29101 cattgttggt atcggatctt ccttcaggcg atttttcccg gatcctctat caaagtctgg
    29161 atgagatttt cagcccgacc cagacgattc atggagtcct tgtcgaagtc tatggcatcg
    29221 gggtcctgat catcggcgac agcggggtcg gaaaaagtga aaccgccttg gagctcatgg
    29281 agcggggaca ccggatcatc agtgatgata cggtgcgcct gcgcaacgtc agtgacacct
    29341 ttttgatcgg gaccggggaa aaccccgccc tcgcccacca tatggaaatc aggggattgg
    29401 gaatcatcaa cttggccaac ctctacggtg ttggagcgat tcgggaccgc aaacagattc
    29461 agctggtgat tcagcttgaa gattgggatg cgacgaaaga gtatgaccgg gtcggaacgg
    29521 aaatgacgac aagctacctc ggaatttcca ttcccctcgt catccttccg gtgaaaccgg
    29581 gtcggaatat ccccattatc atcgaaaccg ccgctcgcaa tgagcgtttg aagaagcttg
    29641 gctatttttc cgccaaggag tttgaccaaa acgtgaagaa gtggctcgaa agcgaatccg
    29701 cccgcaagat ttactacgac gccgaggagg acttttgatg gtacaacgcg agctcgtcgt
    29761 cacgacccga gccggaattc atacccgccc ggccgctaca atcgtaaacg tggcgaatcg
    29821 ctttgaaagc gacctgtttt ttgaacgagg gacgataaag atcaacggaa aatcaatcat
    29881 gggcatcatc accctcggag caacctacca gacttccttg acaatgattt gtgagggaaa
    29941 ggatgaagag gagcttgccg atgcaatcga agccctcttc ctcaaccggt tcgaggagta
    30001 aggatatgat tcgaccattg accaatcgcc aacgggatgt agcgacgttc atcaccgatt
    30061 tcattaaaca aaacagctac ccgccttcgg taagggaggt cgccgagtat tttcagtttt
    30121 cggtgaaagc cgcccatgac cacctcaaag ccttggaagc aaagaaagtg attcgcacga
    30181 cccccggctc cagccgatcg atcgaaatca tcgacgaaga gttcctggaa acggaagaag
    30241 tgatcgagat tccggtcatc ggaacgatcg cagcaggaaa acccctgtta agcgaagaga
    30301 acaccgatta tatgctgaag gttcccgtaa gcatgctcaa aggagcccga gccacctact
    30361 tcgccctgaa ggttcggggc gaatcgatga tcgaagaggg aatcttcgac ggagatctcg
    30421 cgatcatcag gaaaacgcaa agcgccaaca gcggtgcgat cgtggcggcg acggtggagg
    30481 atggcgagga aggaatcacg ctgaaaggct attaccccgg tgaagacatg attgaactca
    30541 ggccggccaa ctccgcgatg ggcccgatct tcacccgctc atgccgggtc cacgggattc
    30601 tccaacttct catcagggac ttttcatgag cagctatctc cttctcggcc ccgaagccgg
    30661 ggcgaaggcg gacgaggtga aacgaattcg aagcgagctg gcggcgaagt acggcgacgg
    30721 cttggagttc gagcgcttct atccgttcga cggggaacag ggagagctgt tcgcttcact
    30781 tgccaacgac tcgctcttca gcgaccatcg cctgattctc atcagccaac tggaaagcgc
    30841 cggcgctgca ttcctcaatg agctggccgg atacttggcc aatccaagcc cgagcgctac
    30901 ggtcgttctt gcgtcgagcg aaacctcgat cacgaagaag atcatggatg cgatccccaa
    30961 ggaacgaacc cgaatcttct atgatctcct ggaaggtgag aaatccgcgt ggatcagaac
    31021 ccacttccgc aggctcggcc ttacgatcac gtccgaagcc gtggagctcc tgatcgccat
    31081 gacggaggac aacacccagg agctcaggac ggtgtgcaac cagctcgccc tcttctggca
    31141 gcacgattcg ccgacgaaac cgatcgacga ggaggcggtt caaacgtacc ttcaccacag
    31201 ccgggtcgaa gatgcgttca ccctcttccc ccaccttgcc gccggcgatc tcaaaaaagc
    31261 cctcgccggt cttcacgccc tgttgggcag cggtgattac cagactccca ttctgctcgt
    31321 cagcggcttg ttgtggcaat ttagacgttt gctttcggtc gccgaaggct ttgaagaggg
    31381 caaggatgaa cccgaggtgt tttcaaacgc ccatgtcctt ctgaaaaaca gcgcaatccg
    31441 gcgtccgaag gataaaacca cctatcggga aggagcgaag cgctactcct cgaaggatat
    31501 ccgttcgatc atcatcgccc tcgcccaagc cgatatcgag gtgaaacaaa gccgaagcga
    31561 agagataccc ctcatcctcg agcggctgat ctaccggatc atcaacaaga agggaaaaga
    31621 gctcactacg gcatccttcg cgacactgct atagggagag ctgtttaatc agccgttgag
    31681 cgacggcaag cggcagcttc gcctctttgg ccagatcctc cggatctttt ttcagcacag
    31741 cctcaaggct tccgtattga gccatcaacg cctcacttcg tttttttccg attccatcga
    31801 cggactgaag caattggaag gaagcggtgc gggagcgggc ggcctggttg gaggcgtggg
    31861 cgaagcgatg gcactcatcc cgaacggcga tgaggagcct cagcgcttca ttgccgtgat
    31921 cgaggcgaag atcctctttc ccgtccccga agacgatcgt ctccgaccct tcgatcagcc
    31981 cgatgacggg aatatcgaaa atccccagat cgtcaaggac ttcccgggcc gcattgactt
    32041 gcccatgtcc tccgtcaatg agcagcagac ccggccgttc cagattttcg ttgagaacct
    32101 tctgatacct gcgggcgacc gcctcgcgga tagaatcata atcgtcgatc cgtccttcga
    32161 cgcttttaat gttgtagcgt cgatagttcg gtttatcggg gtttccgttt cgaaacgaga
    32221 tgagcgatgc gacggtgtat ttgccctgaa ggtgggaaac atcgaagcct tcgatcaaaa
    32281 cgggttcgct ctccagaccg aggagctctt tcaacagatg gagcgcttcg gtgttgtcct
    32341 tgttcctcag tcgtttttcg acatcccgcg tcgcgttctc tcgggccatc cgcaggatac
    32401 gatagtgttt tccctcgatg ggaaggctca cctccaagat gcccccgaag tgctcaagga
    32461 agtagcgccg aatcagatcg acctcgatct catgcgagac gtagaggtat tggggaagct
    32521 tcatgccgtc ggcgtaatac tggatgagaa acgccagaag cgtctccgtc tcgtccccga
    32581 acgtctccgc ccgatagagc gcccgtccga tcacctgccc ctcccgcatc tgcatcaggc
    32641 tgatcgtgca gagcggaccg cgcatctcga tcgcggcgta gtcgcggttt tcttcgctga
    32701 actcctggac ttgctgatcc gttgaaatca tcttcaccgt ttccgcctga tcgcgcttcg
    32761 tcgccgcttc ttcaaagcgc agctccctgc tggctttggt catctcctca tgaagctgag
    32821 tgagcaaccc gtcgaccttt ccctccagaa ggttccgaac ctcggtgatg tatctgccat
    32881 actcctccgc gccgatcaag ccggcgcatg gaccgctgca gagcccgatg tggtagtaca
    32941 gacagggttt ctcccgtttc cgcaagggag tcgaacagta gcggagcgga aagagcttgt
    33001 agatcagatc gaggtattgt tccaacatcc gcgcacccgg gtaggggccg tagtaggttg
    33061 acccgtcgtt gataatctgg cgggttttaa ggactttggg aaacggttca ttggtgattc
    33121 gaatcaccgg ataggatttt ccgtctttca gggagatgtt gtagtgggga gcgtattttt
    33181 tgatgaggtt gttctccagg acgagcgcct catactcgtt tcccgtgatg atgtattcga
    33241 tagtgacgat tttgcggacc aaggcggcgg tcttggcgct ccggttggcc agaaagtagc
    33301 tggtcacccg cttgcgcaaa tccttcgctt tgcccacgta gatgatccga tcatccttgt
    33361 ccttcatcag atacacgccg ctctggccgg gcagctcccg agcctgaagg ctcggagcga
    33421 tcggttcgct gacaaccgtg ttgaaggtct ctatctccga acctacggtg taccgtgttt
    33481 ccttctttgc catggattaa ctgtacccac tcccccgctc tttgacaatt gcagggtcgt
    33541 cggatcgagg agaagtaaaa gctgacatcc ttcgctactt cagtttttcc tcaaagagct
    33601 gaagagcccg gccggtcagg gggttttctt ttccatcaag cacgtctttg agggcttcaa
    33661 gctcgcttgc gctgaacgtt acgctcccga ccttgtgctg ctcgaacgct tcgacggcga
    33721 tcggacatac caagcggatg atcttcaata tctgttcggc gtagacgcgg atctcccatt
    33781 gggcgtgcgg atcgagccga agtttgagga agtggaagag gttgtgcaga tccatctgcc
    33841 agtaccattc ggtgtacatc gagagcggaa ggttgatccg ggcgagctcc cgggccaagc
    33901 ccatctccag aagctgccgg taggtctcat agcttcgctt ctggtcggcg gcaaggatct
    33961 cctggacctt cttggccaag gcgggatcga ccgcttcatc catccggcct tgcttgttat
    34021 cctcgctctg gagattgatg tgccggctgt ccggaacgta gaactcatcc ttcatcactg
    34081 aataccggcc gctgatctcg ttgacccggg cggttcggtg ccggatccac tgccgggcga
    34141 caaagatcgg catcttgatg tggaaggtga agttcacctg ttcaaagggg gaggtatgat
    34201 cgttgcgcaa aaggtagttg atcaatccct tgtcctggcg atacgttttc gttccgtctc
    34261 cgtaggagac ccgggcgctt tgaacaatcc gttcatcgct tccaagatag tcgaccaggc
    34321 ggatgaaccc atgatcgaga accttgaact ccttgtccag gatcgcttcc gccgcttcaa
    34381 ccacgttatg tgccatagct tatctcctcc gtttccaatc taaaggaaac ccaaacgact
    34441 tagcaagacc ccgacggcgg cgctgcagat cgtctcggtc cgaagaatcc gcgttccgaa
    34501 cgaagcgagg atgaaccccc gctccccgag aagaatccgc tcgcggtcgc tgaacccccg
    34561 ttccggcccc accgcaaggg tggctccttg ctccggattc atttcaacgc gatcaaggct
    34621 tatcgaacct tcgacgttgt ccaaggcgat cttgactccc tcatacggat attcctcatc
    34681 cagagaagcg gtaaagacga cttcggggag ctgacaatga ccgctctgca tcgccccgtc
    34741 aaggagaatc gagcggtact cgccgctcgt gtacagcccc gcttggacgt aggacttctc
    34801 cccggtgtcc gtcgggacga gaatcagccg cttgaccccc aacgagaccg cttcgcgcag
    34861 gattctccgc atgctgatcg gcctgaccat cccgaccagc agggtgagcg ggtacagcgg
    34921 ctcagccggt ttttcctcgg tataggtgaa ctcgatgctc ttctccccca cactcgtgat
    34981 cgtggaaacg ccggtggagg tattgatcac cccggctttg aaggagtcgc cttccttcag
    35041 cttcaggatc tttttgatgt gtttggcgcg gtaatcattc aggtcaaggg tgtttctggg
    35101 ttccaacgcc tcaaagagca cgatattcat gcgcagagta tagcatgacg agcacggtcc
    35161 cggtacactc ttgaacatga acctggcaat ttattattca acgagtcacg actgcgcggc
    35221 gaatctggcc ggcgaagcat atttgatgga tttgccctat agccgtattc tgtatttgtg
    35281 ggtaaacgac ccttgtgtcg tcatcggacg ctttcagaac ccccacagcg agtgcaacct
    35341 cgaagcgatg aagaaagacg gagtccaact ggttcgccgt caaagcggcg gaggggcggt
    35401 ctaccacgac cgggggaacc tctgcttcac cctgatcggt gataaggaga ccgccacgaa
    35461 ggaggagaac ttcgtcctcg tcatcgacgc cctcgccgag ctggggatca ccgccgaaca
    35521 aagcggacga aacgatatcc tttttgatgg cagaaagatc agcggcaacg cgttccagac
    35581 gacgaagacg aagttcgtcc accacgggac gctgctcgtg aagagcgatc tctcggtgat
    35641 ggcgaactac ctcaccccaa gtgacaccaa gctcgcttcc aaggcggtga agtccgtcgc
    35701 ctcccgggta gggaacctca aagaagcaaa ggatgatatc accgtgaaag aggttgagtc
    35761 agctttggag cacacattca aagaacgcta tggagaaagc gaagtggttc acatcgactt
    35821 ttccgagttc gagccggcaa gcgctaacta ccggctcttc ggggatcttg atctcatcct
    35881 caagcggacg ccacccttca cccactcctt cacccaccgc ttcgcatggg gcgaggtgac
    35941 gctgaacctc cacgtcatcg aggatacgat aaagatggtc accctcttca ccgattccct
    36001 cgacaccacg ctgagagagc gggtcgaagc gcttctcgtc ggcgtccggt atgaagcggc
    36061 agagatcgcc ggcttgaacg ccggcgaacc cgctctgaat gaattgatcg cctatctgct
    36121 tgcccggctt taacgcccgg taagccggat cagggaggcg aggcgctcga tccgccaagg
    36181 ctcgagcttc aaggaggaga gcctccacga ccagttggcg ctgccgacgg tcgccggggt
    36241 gttcatccgt ccgcttgcgt ccagttccag ccaatcctgc atcggcagga tcgcccagcg
    36301 gcacacgctg tggagcattt gccgaatcat ctgccacacg atctgggcat ccggcgcttc
    36361 aaaataccgc ctcacgacat cctgctcttc cccggtgagg gctctgaacc accccaacgt
    36421 ggtgttgtta tcatgggtac cggtatagat gacggagttt tcgctgcagt tgtagggaag
    36481 gaattcattc gtcggatcga aaacaccctt ttcgaccgta aaagcgaatt ggaagatctt
    36541 catccccggg aaaccgttgg aatcacggag ctcttccaca tccggggtga tgacacccag
    36601 atcctcggcg atgattttca ggtcgtctcc aaggtttttc tttaacgttt taaagagttc
    36661 aactcccggc gacggctccc acgaaccgat ttcggcggtc ggattcccat acggaatctc
    36721 ccaatatgcg gcaaaaccgc ggaagtggtc cacccgaatg atgtcacact gcaggaacgt
    36781 gtgccggaac cgctctgtcc accaggcgaa ctcctctttt acgtggaccg gccaatcata
    36841 caccggattg ccccacattt gtccggtttc gctgaaaaaa tccggcggga caccactgga
    36901 tgcgagttgc ttgccatcct cgtcaaactt gaagagatgg cggttcgccc atgcatcggc
    36961 gctgtcgggg gcgacgaaga tcgggatatc cccgatgatt tctatatccc gctcgtttgc
    37021 ataggagcgg accttcagcc actgtcggta gaaaaagaat tgaaggactt tcacctcttc
    37081 gatttcggcg gaatactgtt tccgagccgc ggcaagagct tcaggttcgc gcaggccgag
    37141 ctccttcggc cagacttcaa accatcggga atcgttgtac actgcgcaca atacgttgtg
    37201 cagggcatag tcttccagcc atgatgcatg atccttgcaa aaacgcttgt actccgaacg
    37261 ttcggacttg ttggcgtgtt tcaggaacga tccggcggct tgggcaagca atggttcttt
    37321 gtatgcccga acggcttgat aatcgatccg atctttgtca aaaacgggaa cggtgtagat
    37381 gtcctcatac tccagatacc catccaccat caactcttca aggtcgataa gcagctcgtt
    37441 tccggcaaag gaggagcggg cggcataggg tgaatcgccg tatcccgtcg ggccgagcgg
    37501 aagaatctgc catagtcgta taccggtctt gtcaagcaga tcgatgcact gaagggcttc
    37561 ctttccaagt gatccgattc catatctgga aggaaggctt gtcgggtgaa gaagcacccc
    37621 ggtctttcgt aatgttgtac tcatgaaagt actatacact attggctttg ccgttggcta
    37681 tctctctttt gtcgccttgt tggaagtcga aatttacggt atactaatgc catgagttta
    37741 tcagaaaaaa cgttgccttt ctcagtcggg gagcatgtcg tatatcccct tcaaggtgtg
    37801 ggtgtgatta cgaagattga ggagatcgat ttccgaggcg tcgatacgct ctacttcgtc
    37861 atctaccttg atatctccga tatgaccgtc agaatcccgg tggataaata ccaggatatg
    37921 ggaatcaggc cgatcgtggg gaaagaagaa tcgcaagcgg cgatcgattc catctccagc
    37981 aagtatgagc cgatgccggt cgattggaag gcgcgctatc agatgaacgt cgatttgttg
    38041 aaagaaggtt cgatcgcttc gatcgccaag gtcgtccaag cgctctacca tcgctccaag
    38101 atcaaggagc ttccggttca ggaacgcaag ctctacgaca ccgcgctgcg gctgttgatc
    38161 gatgaatgtt cgatctccct tgaaaaggat gagaaggaga tcgagatggt cgtattcagt
    38221 cgtttggaat catgatatat ggcatttcca tcccacgcgg tcatcgttac cgctgccggt
    38281 agcagtgacc gatttaatga aagcaaagag atcggagtaa agaaggaata tctcaatctt
    38341 ggcgggcata cggtcctcta tcagagtgtc cgcccgtttc tgggcattcc aaactgccgg
    38401 gcgatcgttg ttacccaccc tgaggggatg gaagaccagt gtgcggtggc ccttgaggac
    38461 ctgctggagc agaacctcgt gccgatcatt ctggtcaaag gcggcaagaa ccgccaagaa
    38521 tcggttctta atggacttga actgctgacc gccctctcgc tgccaatcga gtacgtcgcg
    38581 atccatgacg gtgcccgccc gtatgtgagc gaagaactga ttatcacgac gctggcaacc
    38641 gccacgatct tcggtggagc tgttcccgct ctctcgacaa ccgatgcgat caagacgatc
    38701 gatgagcatg gacagatcat cggtcacagc aaccgtcaac aaacggttgg agtacaaact
    38761 cctcaaatct tccgttatcc ggagatcctc gaagcccatc gccgggcagc cgggactacc
    38821 aatgcatata tcgacgacac ggagatcttt accgattaca accaatgggt cggcatctgt
    38881 gaaggagaac ggacgaacat caagatcacc tacctcaccg atattcccga tgccgaggaa
    38941 cagatcaagc gataccttgc cgacttggag gcgggcaaaa agcaggcaag aataaacgaa
    39001 gcgtttggtt gcgccctttc gcaggcgaag ccgaggagga tgagatgaga atcggaagcg
    39061 gctgggatat tcatcgtctt gaagagggag cgacgctcgt cctcggtgga gtaatcatcc
    39121 cttcggccaa gggcctcgtc gcccacagcg acggggatgt gctgatccat gcgatcatcg
    39181 atgcgattct cggagcgctg gcggaaggcg acatcggaac ccactttccc gataccgacc
    39241 cccgctttga gggagcgtcc tcggtcgatc tgctggatca tgtgataaca aagctccttc
    39301 ccccctactc gatcggcaac atcgacacca cgatcattct caaggaaccg aagctccgtc
    39361 cccatatcga ttcgatccgc tacagcctgg cggatatcct cgaagtcccg atcgatcatg
    39421 tgtcggtgaa ggcgaagacc gccgaaggcc tgctcggtga actcggaacc ggcgatgcga
    39481 tcgaagcgca tgcaacggtt ctgttgacaa tcaaggggta aaaaacttat agtgtggcct
    39541 gcgcccagat ggcggaattg gtagacgcac taggttcagg tcctagcggt agcaatactg
    39601 tgcaagttcg aatcttgttc tgggcatgga aggcagacct catcatcggg tctgcctttt
    39661 agtattgtcg aatgatcgct cttttcccat ttcagccgtc tcgtggtata gtatggcaag
    39721 aggtaatctg atggaagaag gtttgacgct ggagttcaaa aaagagctcg ccgaaggact
    39781 ggagaaagag gtcgtagcat tcttaaatac ccaaggcgga aatatcttga tcggggctga
    39841 tgatggcacc atcctcgggt tggatgatgc aaaatcttta tcactgatag tcattgaccg
    39901 aatcaaaaat aatatccaac ctgcaacgct gggacttttc aatgttgagg tgaagcaagc
    39961 tgaaagcaaa tcgtacatta gagtaattgt cgctcaagga ctggaaaaac catactatat
    40021 aaaaaagcat ggtatgagtt ccaaaggttg tttcattcga attggttcac aatcctcacc
    40081 tatgaaccaa tcgatgatta ccgacctata ttctcggcgt gccattcaaa cattgagcaa
    40141 tgtgctttca ccgaaccaac acctcacgtt cgaccagctc aaaatgtatt acggtattcg
    40201 cgggttcgat gtgagtgggg aattcttcct gagaaatctt ggactctaca ccgacgatgg
    40261 acgtttcaat tatacggcct acctcatgtc tgatgataat ggggtctcaa taaaagtcgc
    40321 tcggttcaga ggaatgaata agctcgatat cattgagcga aatgaattcg gcagaacgtg
    40381 tctcatcaga tccgcatatc aggttctcga taaacttgag gtcgccaatg ccacagttgt
    40441 acgagttggc ggaaaggcag caagaaaaga gatacgcctc gttgatagag atgctcttcg
    40501 tgaagcggta cttaatgcca tcatccacaa tgactatatt ttaggatcat ttccagtctt
    40561 cgaaatctat gatgatcgta ttgaaatcgt ttcctccggg gggctgccga taggtctctc
    40621 acaagaagaa tttttcaaag gcctctccca tccaagaaac agagagctca tgaggatttt
    40681 ttctgatatg gatctttgtg agcaacttgg ttcaggcatg caaaaaatct tgagagcgta
    40741 tccaagagat atattctcca tttccgacca tttcatctct gtccgcttcc cctataacga
    40801 agaagccctt tcaatatatc gtgaagagga tccatgtaaa ccatctggcg gtaatagtgg
    40861 cggtgatggc ggtaataatg gcggtaatac aatcactatg ctcccagccg aggatgacgg
    40921 gctttcagaa aatgcattga ccgttctcag agctttgcaa actgacggat ctctcagcca
    40981 acggcaactg tctgaatcca tagatatgtc gatgagaacc gtccaacggg cactgacgga
    41041 gttgcgtgat gcccgatata tagttcgcga aggatctccg agatccggaa catacatcgt
    41101 gttgaaacag gtatgactcc ggtgccgcat atggacatgt cccgacgttg ctgaaccgtg
    41161 aaaacgggat tggcgccaag gctcattgtc cgatgatcgg cattgatgat ggagcagagc
    41221 gaacacaagg aactctggat caatcatttt gccagcgctt caaacgcttt acgatgtttt
    41281 tccagaatac gtcttgcgac gatctcaatc ttttcagcgt cagtcagatt caaaaacgtc
    41341 tcctgtgcaa ggtcgatgac cagatacttg ggacgattgt ttttgaagat gacggcgtta
    41401 ccttgcgcat ctgcaagccg tgcgacttta gaaaaattct gattagcctc cgtcatcgag
    41461 acaatctgtt ttgtattgat aatcatcatg acacctcttt cataagaata atacttttct
    41521 aggatataaa caacctatat atactcctct accgtacaga tagcaagaca tcgtcagcgg
    41581 atcatggttt gccggttttc gatcccggaa atcgatgtgc ttgccatcga tcaattcctc
    41641 gtatcgaaga gaatagtcga ggcaagacgt atcgatcacg tttggaaaaa gctccctcga
    41701 ctagtttgcc tccgaatact gagcattggt gggattgaag cgggcgatga tccaatcgct
    41761 cagattggga aagaagcgat agaggaatga ggagaaatgc cccaaggggg tgagaaccat
    41821 ccgtttcttt ctccttttga tcatcttgag catatatctg gctacatcct gttgcgtgtg
    41881 ggcgtttttg cgcttggaaa gaggcatcag cgatccatcc gcactgtaga tcaccttctt
    41941 cggatcgttt tcggtgaagc cgacgtagat gatgccgatg tggactccct tggagctcac
    42001 ttctgcccgc aggctctccg tataggcggt aagcgcaccc ttgcccgccg catagcctgc
    42061 gaccttgggg acaccgtgga gagcgaccag actggaggtg aagatgatgc ttcccttctg
    42121 tcccgttata tgtgggagag cgtaataggt catataggcg ctgcccagaa agttgatgtg
    42181 caccatcgcc tccagcacct caggagtggt ttcattgacc cttccccgca tcgacattcc
    42241 ggcgttgttg atcagaagat ccagccttcc gtgacgagtg atcacctcat cgatcgctcg
    42301 tttgcacgca aggtggtcgg tgacgtccgc ctgaatggag aaaagaacct tttgctcggg
    42361 atcaaggaga ttggccgttt cctccagcgc ttcaacatcg cggccgagga tgtagacgat
    42421 catcccttct ttgagacaaa gtcgagccgc ttcccgtccg atgcctttgc tgccaccggt
    42481 gatcaacgct accttcatat cgcccctcca tcgagagtgt atcgttggca tgtgtaaaat
    42541 caaagactat tgaacggaat ataagagtat tgagtcttta ctatctccaa aaagtacgat
    42601 attatcatac ttataggaga taatatgctc aaaccctatg atttggtgat gtatgacctg
    42661 agatcctacg cctctccaaa ggcgaagctt actcagatga tacggaaagg agagatcatc
    42721 cggattgcgc gcggagtgta tgcggactcc ttcgatgatc cacgcctgcc cgtcgccggc
    42781 atgctcgtga gccccagtta tgtctctttt gaaacggctc tggggtatta tggatgtatt
    42841 cccgagcggg tgcatgcgat caaaagcgcg ggattcaacc tgaacaaaga gaagcgtttt
    42901 gacactcctt tcggcctgta cacatttcat tacatccctc ggaacgtgtt ccccctggcg
    42961 ctcacacccg ctgaagaaca agggcatggc ttcaggcttg cccaaaagga aaaagccttg
    43021 ttggatacgc tttacaaaat tcggggaatc acgacaaaaa aggggatttc agacctcctc
    43081 tttgaagatc ttcgaatcaa tgaactcgta tttgaggaac tggactggga gctgctatcc
    43141 gcattggctc catactaccg gagcacaacg atcaacacct tgatccggca cgggagaaac
    43201 atatgagaaa cgaagtaacg gcaatggtta atcttcagcg ttccaactcg cctattgaag
    43261 cagtcaacgc gttaaaagaa gcaatccagg aattggttct taaagccctt tcaaaaacgg
    43321 attttttcaa ccgcgccgct ttttacggag gaacagcatt gagaattttt catggtcttc
    43381 ccagattctc tgaagacatg gatttctcgc tcgtcaagcc ggatccgaca ttccgattgt
    43441 cggcctattt cagatcgatt gaagaagagc tgggctccta tggctttgaa ctgaaattca
    43501 tatcaaaaga aaaacgaacc gtctctccga tacaatccgc atttctcaag ggaaatactg
    43561 aataccatct catcaagttt ctggaacttc aacgaccaat cagcggtgtt gcccgaaatt
    43621 cgatcatcac gatcaaactg gaagttgacg tagctcctcc ggacggagca acatttgagc
    43681 gacgatttcg cctgctgcca tccccctatt ccgccctgct gtatgatgct ccttcgctgt
    43741 ttgcaggaaa attgcatgcg cttctgtgca ggaaatgggc gaccagagaa aaaggaaggg
    43801 acttttatga ttatgtctgg tacctccaac acaagatacc cgtgaatctt ccccacctcg
    43861 aagaacggat gagacaatcc ggtgattgga tgaacaatga caagctcaca aaagcagacc
    43921 ttatgcacct tctccatgac cgttttctgc gaacggatta ccatctcgtt acacaagata
    43981 tccttccttt tatcaaggac tcctcatcgc tcgatcttta tgggactgat ttttttaatt
    44041 caattaccga tgagtacctt caaattgttc agtagatcca attcccaaca tttcgccctt
    44101 ggcggtgtca ttgttattat acggttttgg gaaagaggca ccgcctggtc tctgaattgg
    44161 acttgctttg acttcaagtt ggtggtttaa tggggtatac gctcctgtca gttctttttg
    44221 attgtttgtg caccaaggag gcggcgtgtc gatgatcgac acaagaagaa ggtgctaccc
    44281 gattcgtagc acatcgaccg tttttggatg cagtgggggc aacgatcccc catccatcat
    44341 aggcaacact ttcaaaagga gaaaagtatg cgttcaaact taaaagtgat cggcatggtg
    44401 ctgatcgtgg ctctcgtcat gggcggactg ttcgctcagg gagcaaaaga cgaaggtgca
    44461 gtcgccaaca aggataaacc ccttgtatgg ttcaaccgac aaccttcaga ccccgtgacc
    44521 ggcaaaatcg acatggactc gatgaactgg aacagcaaga cctattatgt cggattcgat
    44581 gccgcaggcg gtggtgcagt gcaaggtaaa ttgattaccg attatcttgc cgccgccaat
    44641 cccgccaagc tggaccgcaa tggcgacggt acaatcgggt acgttctctg catcggtgac
    44701 gtcggccata acgactccaa agcccgcacc gagggtattc gaaaagcatt gggcacctgg
    44761 aacggatcga ctgaccccgg tgctgcaaaa gtcggatcgg ttgttgtcgg cggcaagacg
    44821 atgagagtcg tcgagctgga aggcaaagcg atgaccggca ccgacggttc gacctggaac
    44881 gccaacgcag cgaccgaggc gatgggcgga tgggcgacga agttcggcaa ccagatcgac
    44941 atggtcgtct ccaataacga cggtatggcg atgggcagcc ttcaggcttc caactacccc
    45001 gccggagtgc cgattttcgg ctatgacgcg aacgccgacg cgatcgaggc gatcggagca
    45061 ggccgcctca ccggaaccgt ttctcagaac gtcgacgctc aggcagccgg aacccttcag
    45121 gttctccgca acctcctcga cggccttacc ggagccgatg tctacaagaa aggcatcacc
    45181 gaagccgacc agtggggcaa caagattacc gctccgctcg actattgggc ggatgtcaaa
    45241 gcggtcatgg ccgccaactc cggtgttgat tccaccaact ggaaacagta taccgaagga
    45301 agccgagacg ccggcatcaa gcagagtgca gccccgaaga agcgggttct cctgacgatc
    45361 tacaacgcca gtgacaactt cctttcctcc tcctatgtgc ctgcgctcca gtactatgca
    45421 ccgctgatcg gaatcgatct cacgatcgtc cagggcgacg gacagaacga atcctcaacc
    45481 ttggataagt tcaccaacct tggtagcttt gacggctatg ccatcaacat ggtcaagacc
    45541 aactccggtc ccgactattt ggataagctc cggtactaaa agcaccaatt ttgaacagga
    45601 agcatgcggg gcgggacgtt cccgcatgct tttccaaatc tacctgagaa tgccatgcaa
    45661 gaaccagtat tagagatcaa ctacctctca aaatccttcg ccaaaaacaa agtcctcgac
    45721 aacgtcgcgg tcaccgtcca caaaggtcga gtcctcggcc tcatggggga aaacggggcg
    45781 ggcaaatcga cattggtcaa atgccttttc ggcatctacg ccaaagatga aggacagatg
    45841 tttttaaacg gcaagccaat cgacttcaag aaccccaagg aagcactcga aaacggggtc
    45901 gcaatggtgc accaggaact gaatcagtgc ctggaccgct cgataaccga caaccttttc
    45961 cttggccgat accccttgcg cttcggcgtg atcgacgaag cccggatgct gatggaagca
    46021 aaccagctgt tcgcatccct gaacatgaac atcaaccccc gaaccatcat gaggacaatg
    46081 agcgtttcac accgccagat ggtcgaaatc gccaaagcgg tctcctatga tgcgaagatc
    46141 atcgtccttg acgagccgac ctcttcgctc accgagcgtg aagtgaagaa gctcttctcg
    46201 atcatcgatg atttgcgcaa aaaaggagtc tcctttattt acatatcgca caagatggat
    46261 gaggtcttcg agatctgtga cgatgttgaa gtgctccggg acggaaagat ggttctttcc
    46321 aagcccgttg ctgaaaccac catggatgag atcgtctccg cccttgtcgg ccgctccctt
    46381 gacaagcgct accccgatgt cgacaacaac cccggggaag agtacttcca tatccgcaat
    46441 ctgactacga agtttgaccc cgttttgcag gacatctcct tctccgttcg aaaaggcgag
    46501 attttcggcc tctacgggct ggtcggagca ggaagaagcg aactgctcga agcgctcttc
    46561 ggaatcagga cgatcgccag cggtgaaatc gtgttgggca ataaaaagct ccgttttaaa
    46621 aatgcaaaag aggcgatggc gtatgacttt gccctgatta ccgaagaacg aaagctcaac
    46681 gggatgttcg gcaaggatac gatcaagttc aacacggtca tcaccaacct ggaaagctat
    46741 aagacaatga acgtcctttc caatcggaaa atgttggatg ccgccaaccg tcagattgtt
    46801 caaatgagaa caaagtgcgt ctctgccgaa gagctcatct cctcactcag tggagggaac
    46861 cagcaaaaag tgattatcgg caaatggctt gaacgtcaac cggaaatctt tttgatggat
    46921 gagccgaccc gcggtatcga tgtcggagca aaatatgaga tctatcaact cattatccga
    46981 atggccaaag aaggaaagac gatcattgtc gtttccagcg aaatgccgga gattctggga
    47041 atcaccaacc ggatcgcggt gatgtccaac cgccggctgg cgggaatcgt gaatacgaat
    47101 gaaacgaacc aggaagagct catcaggctc tccgctaaat acttgtaatg aggtgtgctg
    47161 aatgaatgcc gactatcaag aaatcatgga attgaccgaa gatccaaaga ttattgaata
    47221 ctcccaaaca ttgaacaaac ttcgtgaaaa cggagtgaac aaaatcaaga agctgcgcca
    47281 ggatatcgtc gccctgcgca agagcaagat ggttcatcct caggagaaac gaaaacagat
    47341 caaagcgtgg aaagaggaaa ttcatcttgc caagaaggac gctgcacaga acaaagctgc
    47401 gattgatgag ctggtgaagg aaagtgtcgc gtatgccaac aaggcggcca agacctttat
    47461 tgaacaggta acgatccgtg aagacgaggc gatcgcaaaa gcgaaacagg cgtatctgga
    47521 agaggtccgg acaatcaaag aggaggccaa acgctcagag accgcaatcc ggtcggaata
    47581 caaaggccgc agtcgaaaag agctcaaagc ggaacttgaa gcgcaccggt ataagacgaa
    47641 aaccgccctc ttcgatgcga ggagtcatcg ccagcaggcg attgatcagg cgctggcggc
    47701 aaaaaaccaa gcgtttgtcg accatgttca aacaaaccgg aaccttagaa acggaaaaac
    47761 gaagttcagc gaagaccgtc agctcaaacg ccgcgaatat cgctacaact tcaagctctc
    47821 acagttcctt cttgccaacg gcctctatat agcaatcggg atcttcttca tcgtcgtcat
    47881 cattctcgct cccctctccg gtgccggcaa ccttctcaca ctgcccaata ttctgacgat
    47941 tctcgaacag gcttcaacaa gaatgttctt tgccctcggt gtagcgggtt tgatcctctt
    48001 ggcgggaacc gacctcagtg tcggtcgaat ggtcgccctt ggagcggtaa ccaccggtct
    48061 catccttcac cccggcaaaa atatcgtttc cgtcttccgc tatccgacat gggacttcac
    48121 cccgatggcg atgagcaacc gagtgcttat ggctctcggt ctttcgattc ttctctgtgt
    48181 cgcattcagt tcctttgccg gtgtattcac cgcccggctg aagattcacc cctttatctc
    48241 cacccttgcc acccagttaa tcatctacgg gcttctcttc ttcggaacca gcgggactcc
    48301 cgtcggatcg atcgaccgca acatcaagga tgcgatcgga ggccggtgga ttctgggtca
    48361 gatcggcagt cagtatgtga cgttcccaaa actcatcata cccgccctct tcgcgatctt
    48421 catcgcatgg ttcatctgga ataaaacaat ctttggcaag aacatgtacg ccgtcggcgg
    48481 aaacgccgaa gccgcaagtg tcagtggaat ctccgttttc aaggtcacga tgggcgtctt
    48541 catcatggcg ggaatcttct acggctcggg ggcattcctt gaagctttcc gggcgaacgc
    48601 ctcggcggga accggtcagg gctatgagct tgacgcgatc gccgcgtgtg tcgttggagg
    48661 aatctccttc aatggaggaa tcggaaagat cagcggagca gtcgtcggtg tcatcatctt
    48721 caccggcctc acctactgtc tgaccttcct gggcatcgac accaacctgc agtttgtctt
    48781 caaggggttc atcatcatca ccgccgtcgc actggacagc gtgaagtacc tgaagaagaa
    48841 ataaccttca gcgcatccga attcatcaca ggccaccttc aggtggtctg tggtgcttat
    48901 acatcacatt aaaaatgtta gctgatcgtt tgtttttacg tcccttccat gagttcgatt
    48961 agacacgtct tctcatgcga accgaatgta ataacgctgc ctgaaggtgt ttgcgttacc
    49021 tctccagcag agtgagaccg gatcttgtaa atatttctta agcaaacagc ccatttccta
    49081 agggatcctt cggctgtaac ttcaagggtg gatcggcatc gagtcaccgt gcaggaagcg
    49141 aggagtgatc catcgtgtgc aaacaccttt gcaccggtcg gtgtattctc attaagggaa
    49201 aatacatgaa acgtagtatc cgtattgtat gagtatgcca cctcggtttc atttgctcca
    49261 atggcaagca ccgtgtttgg tcggacaaaa agcggcaggc taaaatagtc gtatgtttga
    49321 gatatatacc taccaccttc gatctcctca ttactgagca agtgagtcca acgccccttc
    49381 ggaaggtaga actctacttt cccatccgct gaaaataccg gagccacaag aagagattct
    49441 ccaagcatat actgccgatc cagatacaat gttgcgggct cctcggagaa ttcaagaacc
    49501 atcgctctca tcatgggaat accctcttgg tgggcaacaa cagcttgact gaagagatat
    49561 ggcatcaggg tgcacttcaa cctcgtgaag aaccccaaca cttcactggc ctcttcatca
    49621 aagttccaag gaacccgata ggattcgttc ccgtgcagtc tgctgtgagt tgagagcaat
    49681 ccaaaactta tccatcgctt gaagagatca ggggtcgcag tctgttcaaa tccaccgatg
    49741 tcatggctcc agtagccaaa accacataat gagagcgaaa ggccgccacg taagctttcc
    49801 gccatggatt catacgttgc tgaacaatct ccaccccagt gaacgggaaa cttctgaccg
    49861 ccgactgtag cggatcgagc aaaaacaata gcctcgccaa ccccacgctc atgttcaaga
    49921 agagagaata ccgcttcatt gtacaggtag gtataatagt tgtgcatcaa atgaggatcg
    49981 ctttcatcat ggtacacaac attggtggga atccgttctc caaaatccgt tttaaaggaa
    50041 tcgaccccta tatcaagtaa agctttcagc ttgctttgat accatgtgac cgcttgcgga
    50101 ttggtaaaat ccaccaaccc cattccagct tgccatcgat cccattgcca gacgcttcca
    50161 tcgggatttt tcaccagata gcccttttcc atcccctcat caaaaagcga tgacttttgg
    50221 gctatatagg gatttatcca aacacaaacc ttgagtccac ggtcatgtaa accagctata
    50281 agagctttcg gattgggaaa ggttaccgga ttccaaagca gatcgaccca ttggaactcc
    50341 ttcatccaaa agcaatcaaa atgaaataca tgcaaaggta tttttcgctg ctccatccca
    50401 tcaataaagg agtgaaccgt ttcttcgttg taattggtga caaacgaggt tgaaagccag
    50461 agtccaaaag accaaggagg taccaatgcc ggtgttcccg taagcttggt gtagttggaa
    50521 agaacctgtt taaggctttt tccaccgatg acatagtatt ccaggcattg tccggaaaca
    50581 gagaattggg cagcggttac gacttcactg gccagttcaa atgatactct gccagcatca
    50641 gcgaccaaca ccccatatcc ctttgaagac agatagaagg gaatgttttt atatgcctgt
    50701 tcgctactcg tccccccatc ctcattccag atatccacga cttgcccatt ctttacaaac
    50761 ggagtaaagc gttcccctaa accatagagt gattcgccta caccgagact caagtattca
    50821 acctggaatg ggttgttatg atccacaact gcatgaccac tcagatgacc gagtgaacga
    50881 gtaagctcct tctctttgta cctgaatgta taatcacagt tcttttcggc attgatcatg
    50941 attgataaag atccgcttgt tgcaaccgcc ttggtgtcat ttttttgaat gatcaagtga
    51001 gaaggaacgt ctttaaccac atcaaagaaa ggtcctttat tcaaaaatcc tttaaaatgg
    51061 taggatttga catgaagtac atcaggcatt ggtgaggtga tttctgtcgt aagcaacgga
    51121 gtattcaacg ttgacccgcg atgaagcatt ttcttaaccg ctgaaaaaat cgtcagtttc
    51181 ccttcatctt gctgcatatc gacgatatca actcggttca tatattgtgt attattcttt
    51241 atatgccaat atccatctct aaacttcatc gtattactcc caaaagaaaa tattatgatt
    51301 tgactgcgcc agccaccatt cctttaatga tgtagcgctg taaactcaag tagaaaatga
    51361 tagccggcaa aattgtaaga agtactccgg ccatcgcata gttccaatca attttataaa
    51421 gaccaaagaa attatatgca gccaactgca acgtcaatga tttcttcgaa ccactgagta
    51481 ccaatagtgg caacaggaaa tcattccaca tccacatcgt attgatgaca attaccgaca
    51541 ctgttaccgg ttggagcaat ggaaaaatga tttgaaaaaa ggttctcaat ggtgaagcgc
    51601 cgtcaattga agcgcaatca tcaagctcgc gtggaacgga tttcacagca ccatgataca
    51661 tgaagagtgc caaagaagcg ccaagccccc aatacagtac tccaagtcct gcaggagatc
    51721 cgatgagttt taactcttta gcgactttca ctaaagagat catgaatgaa tggaatggaa
    51781 tcatcattgg agcaatgaga accataaaca taataaagct tattcgagaa ttggttcgag
    51841 ataatttata acccgccatt gaactgatcg aaacaacccc taaaacgccg atcgatgtgg
    51901 cggcaagggt atttaaaaag tatcgagggt aattcataac tttaaaagtg tttgcaaagt
    51961 tatgccattc caacgaggtt ggcaacgcaa tgacattact cgttatctct tgaaatgtct
    52021 tgaatgagtt tatcaagaca aggaagagcg gatagagata cactgtggca atcagcgtta
    52081 acacgattgt ccatacataa gggagaatga agcgttcctt catacctcta cctccctttg
    52141 cttgaatatc tggacttgga agatggagag aacaagaatc atgagaaaca ggacaactga
    52201 tttcgcagta ccatatccga atctattgat cacgaacgca gttcgataaa tatcgagggc
    52261 aatcgttgtc gttacattac cggggccacc gtttgtaagg ctgaaaatga tatcaaatga
    52321 cttcaatcca tttgacagcg aatgaaatac acttactgtt atggagggaa caatcaacgg
    52381 aagcgttacg ttaaaaaatc gttttgtttt attcgctccg tcaatcatcg ccgcctctat
    52441 taattcatgg ggtacggttt gcaaaccggc aatgtagatt accagataaa aacccagcga
    52501 atgccagatt gataccatta atacactaaa atagactact ttcacgtctc ccaaccagct
    52561 aagcataaaa atctctaacc cggttttttc atataatgag ttgaacccgg cagaaaaaat
    52621 aaaacgccag atatatccga ttatgatcaa actgataata ttcggtacat aaaaagcagc
    52681 cctgagggca tttttcccct tgacatcact gtcaagaatc actgcaagca ataaagccaa
    52741 tatgttaaca aggacaacgc ttccgagtgt aagctttaga gtaaatccga aggcacccca
    52801 catcttaggg tcatcggtga agagttcatg ataattttga aatccaataa atgtttgagc
    52861 tttatctaaa ccattccatt tcgtaaatga agaaaaaaca ctcatgagaa agggaatttc
    52921 gacagagatg agcaccatta gaaacgcagg tgccgtaaac aatagaaaca ccaacgttct
    52981 attgaatgtt ttacgctgta gctgagtcat tgtacattcc tatcatgtgg ccgggcctta
    53041 gcccgggcca cattcatcaa aacgattaca ttgctcccgc aattttagca taggcggaat
    53101 ctagggcatc aagagtttga gctcgatccg ttaagcccgc acaataaccc tgaaggattg
    53161 cacccagttc ttgctcggtt ccggttggga acatctgata gaaccaagga tatcccggag
    53221 ccccggccgc catgagtttg gtagtctctt ctgcgaccat ggaatcgggt gctgcagcac
    53281 cgatgattgg agaaagctgc tttactttag aaggtatcca agcctttcca tattcgctgg
    53341 tcgtgagcca tcggagccaa tcgagggctg cctgaaggtt ctttgagtct ttggcaattc
    53401 taattgtctg gttcgaatca aacatgactc ccgccgtcgt tgcattgttt ccggtaggac
    53461 caacaaggaa gccgatctcc accgaagggt taatcccccg aatcgtatct tccgcccagt
    53521 ttccttggtg aatcattgcc actcggccgg tagccaagcc agcggtctga tcattgaaat
    53581 ctgattcact aggcttgttt ccaccgtatc gcttgatcag atctaacaag tcaaaaatcc
    53641 gagacatttc aggaatatct ttgaacttca gctgaccact attaagcatc gccacaaatt
    53701 gttcatatcc accgtatttt gatacaggca catcggcaat tgcttgccat gcggtttggg
    53761 gaagaaccca aaactcatgg aatcccgtag caaacggctg gactccgtta gcctcaagtt
    53821 ttttggaaac ggcttcgtac tctgcaaatg ttcgcggtaa atctgtgatc ccattgtccc
    53881 taaacacctt cttgttatag atgaacgaat gggattgaac taagaaggga tatcccagaa
    53941 cttttccatc caaagtgaca cctgcaagcg taccgggttc gatcaatgaa ataaaatcct
    54001 cattcgtcag gtcatacaca taatctttgt aggtagagtt gtctgcgtat gcactgctca
    54061 tgaagatatc gggaacatct ccggaattga gtctacttgt aagaacggat cggtagtctg
    54121 tttgtaagac ggtgatatca agggtaaccc cagtctccgc tttataggca gcaaccatgt
    54181 cattgtattg ctcagctagc tcgggactat agaagagcac atcaagagtt gttgcctttt
    54241 cctttttgtc ggcttgtcct ccggcaaaca tggttgtcat cgctaaaaag acaaccagtg
    54301 agaaaatgag aacttttttc atattccact ccttttttgg tgctattacc acaaatatgt
    54361 ggtatgttat acgtataaca tacaaatatt ctgctgtcaa ttaaataatt tttatgcgag
    54421 attaaggtaa tatgggtatt acgcaaaaag aaatagctaa acatctcgga gtttcctata
    54481 tgacggtgag tagggtgtta aacaactcga atgtttacgt ctccgaccat cttagaaaaa
    54541 aaatcctcga ctatgcagaa gagatgggat atgagccgca tcgggccagt caggtactgg
    54601 tacgcaacaa aaaacgacat atagccatct tctcctctac cctgccaacc tatttctggg
    54661 aagaaataaa caaaggtgtt cagattgcag caacacagat taaaccgttt gactatacgg
    54721 tacgatatca tcaggttcct gaattggata cggaagcgta tcttgccctc atcaagcaag
    54781 aactgaagca gggtgtcgat gctatcgggc tggtaaatca acgcatgtat gatatggaca
    54841 gaatcatcgc tctcattact gaagcccaaa ttccttatat attcttcaat gtcgattctc
    54901 cgacaaacaa tcgtctttgc tatgttgggt ccgattatcg tgatggtggc cgtttagccg
    54961 caaacttcat tgcggttact ctccagcttt ctccaaacaa agaggtatta gttctccaag
    55021 caacagaatc aaaacttcaa agttcacgag cccctgacat caacaagcta cgactagagg
    55081 ggttcatgga gctcgtcaca acaaaacata gtaacatttc agtacacgta gagttttttg
    55141 attctctcca acaagacacc gaaacattta cacaaatcca tgaaataatc caacgatatc
    55201 accagaaggt atcagcaatc tatctgattc ccgcttttaa tacggaattc ctcagagcat
    55261 taaaagaaat accaaaatcc aatatcatca caatcctgca tgacttggac agcactgcaa
    55321 gctcacatct taaaagcggg gaattatctg cagtaatcga tcaaagccct actcttcagg
    55381 gatattacac cgtgatgaca ttggaaaagc ttcttgagcg aggatctgaa catattttgt
    55441 cattcgtcaa agtcgaccat aatcttgtac tcaatgagaa ccggcagctg attcatggtc
    55501 ttatcgaagg gcaatttctc atttagcccc agaaaggcct tgcgctcact acatgaccgt
    55561 atgtgaatgt gatgcttgat cattccagtt tcatctcgaa gtcgtactcg ggtggaatcg
    55621 gattgcgggc aagcactttc tttcgctcat catccgtgat ctcttcaatt ccatgcttct
    55681 tgcaataccg cagaacgcac atctcgttcc acagttcggc caagggggtc tccccctccc
    55741 ggatatgggc gagggttcgg tcaaacacca gagattggat cagctccgga ttgccttccc
    55801 acaccgcaag acgggcccgg gcaagtcgga gcgcttcgct ttcttccagc cagcggggcg
    55861 ggatcgaatc gaagactacc ctgacggttt cgaacgcttc ggtcgtcaga agcagtgaaa
    55921 gatactctga agcgatcgcc caatcctctt cgaatccggg aagcatcgtg gatttctcat
    55981 accagacaag agcctctggc agttcagaac ggcggagttc aagctgtccg aggtttcgtg
    56041 cggtatacgc cgagggatgc agatccaatg attgaaggaa catcgtctcg gccagagaga
    56101 cctgttcctc ctcaaggtag atgattccca gaacgtgcag gaggctcgcc cgcccggcat
    56161 cgctcatctc tttggagaga gcctcttcaa agcgtttctt ccagcgatgc gagaccggtg
    56221 gcacgaatgt ggaagatccg ccgagggacg gtagcaggcc gctttcgaga aactcgaggt
    56281 agaggcgttc ctcggctccc acatccgccg cttcaaagcg gaacgccggc ggaagttctt
    56341 ctgtttggaa gcgcgtttcc agataccccc acccctctcc ccgatgcaga atggagcccg
    56401 ggcgaattga agcggatcga gtcacttcac ggtcgatctc cttgagtgag gccagattga
    56461 tccgacgagt caccgcctct tccacgctcc ttcgagctac ttcataatcg agttcatgag
    56521 ctttgcccgg atcgaccttc agcagcccga acgcctgtgt ccatgaaaca ctacttcggg
    56581 cggggaggat ttcaccgtgg agctgcgtgg gggcgacccc gctttgaatc tcgacatagt
    56641 cgatggtgct gtccggagag aggtagcgct gccatcgttt ccccccgctg tgggttcccc
    56701 aacagaacat cttccgatag gaaagaggat gggtggaagc ttcaaacaat ccttgcccgt
    56761 cttttccgag agcacactcc caaggcagtt catcatcttc gcaggtgaag aagtactcgt
    56821 tggaagcaat gaagcgattg ggataggaaa catcgatccc ctgatagatc ggcatcgtcg
    56881 gcatctccat gatgccgaaa agccgctcat cgttggggac ataggaatcg aggtagatcg
    56941 ctttcggatt cgatgccaga actctcaggt actcatcggt ctccaccgcg atgttgaccc
    57001 acgcatagag cgaggcatcg gtctccttga gattatgaac ggcggtatgg acgcagagca
    57061 gatcccctct caggtggaaa tcgagatgaa agtacagttc ttttgcccgt tcaaaggagt
    57121 agaggcgaag gaactgccgt ccttcctcat ccttttgcac acttgcatgc accggcagat
    57181 tcgttgaaaa ggcgtgtccg tactggccga tgttccattc gatcccgccg gcgaaccatg
    57241 catcgagaat cgcaagattt ccgacctgaa agtttttatt tgcatacaag agttcccgtt
    57301 tctcagcctt gttgaacaga ctgatcagcc tgcccccgag cgaaggcagg aacgttaccg
    57361 ccaaaaactc attctccagc acgatcgccg gaatatcggt ggcggtccgg ttgcggtcat
    57421 agcgatcctg catccgatag ggaagcgccc gccttccgca atcaagaccg aggtatctcc
    57481 ggtatcgttt tggaacatcc tctttcagcc ggacgggaag atcgtgatca ggatcgcgaa
    57541 acgaaggcaa gggactgctt ccggcagcaa gcgcggcttg ggcccggtag accgttttcc
    57601 gtatcgtggt cattgttcac cccctatccg aacaatcgat ccatgtcgcg catcaaggcg
    57661 atctggtttc gggcacggat gagattgtgc tcttcatact cggtgtgata gtaggcatcc
    57721 ccgccaagat aatccgtcag gaagcgaacc gccataatct gcgtgatgtt tctccccgat
    57781 tccactacca actcccgctc ttcatgggtg agaaaccctg cgtacgagaa atatccttcc
    57841 aagagcgcgt catggaacgc tttgttgcag tggacgaggc ttacatcttt ttcatcttcc
    57901 acggcggtcg tagtcgcggt gcggatcatg tctccgctgt caaacaggat cgttcccggc
    57961 atcacggtgt caagatcgat gatgcagagt gcctccaacc cgtcttcgct gaacaggacg
    58021 ttggagatct tcgtgtcatt atgggtcacc cgaaccggca gttgaccgct cttgaagccg
    58081 tcgatgagca catacccccg cttccggttt tgatcaaggt actccagctc agggcggacg
    58141 tcttttgccc gattgaatcg atcaagggtg agagcttcct tgagctggtc ataacggact
    58201 cgcatatcat ggaaatcggc gatcgtctga tgaaggcgcg agccgtcaaa atctgccagc
    58261 tgggtctgga acgtcccaac cgccttcccc aataaatacg cttgattgac attttcgacc
    58321 cgagggatgg tcttcacccc gtcgatgtag cgataggttc gccagaagtt gcccgcttca
    58381 tcgcgatgaa aaagcccttc atccttcgtt ggaacgatga ccagacaccg cttggagtaa
    58441 tcgtcgtacg tttgtgcaag ctttgcctga atatgggagg tgacaaggtg gatgttctcc
    58501 atcacctgcg gagggttctt gaagacaaag tggttgatgc gctgatgggt ataccgtcga
    58561 accgttccct gctcatcgaa ggtcgaaaag aacgtatcgt tgatatggcc ctcattggtt
    58621 tgcttcagag cgaccaaccg cccttcaatg tgaaagcggg cgatgatgtc ggcaaggtcg
    58681 gagtggcttt ggtgcataca taatccttgg gtgtaaaact agcctttgat ggctccggcg
    58741 gtgactcctt ggataatccg ctctgagagg aatatataca agataaatgt cggagcaaag
    58801 acgatgacga cggaggcaaa catccctccc caatcgcccg tgtagcgcat cgcttggatc
    58861 atgttgaaca atccgaccga taccggacga accgtggtct tgttcgcaaa gatcatcgac
    58921 atgaaatatt cattccagat cgtgatgaag ttgaacaccg tgaccgtgat gattcccggc
    58981 tgcacaagcg gtagcatgat ccgccagaac gtccccgtcg gcgaacatcc gtcgatcgcc
    59041 gcggcctcct cataggtgga agaaagattc ttgaagaacg agagcagaaa gaacgttgca
    59101 aaggggacat tcatcgagac atagaggaag atcagtagcc agcggttgtt ggtgaggcgc
    59161 agccggctga ccaatccgaa cagcggcatg acgatcatga tcgcgggaat ccccaatgca
    59221 ctggcgatca tgttttgaat cagaaggttt cccttgaagc ggaaccggct gagcacgtat
    59281 gcggcagggg cagcaatcaa gatgatcgcc gtgcagctga ttacggtata cagaaccgag
    59341 ttcataaagt agacggaaac tttttgcgtc cgccacgctc gagcatagtt ttcaaagtgg
    59401 aagccacttt caaagcggaa catatggtcg ctgaagatct cctggctggt tgaaagggag
    59461 gcgaatatca cccatccgat caagacaaag gtgaagacca cccaggttac aatcacgata
    59521 taggagggaa tcaagcgttt ttggttgcgc atttcaaacg taacggattt acgagccata
    59581 tcgacctcct acaattcgac atcatcgttg cgcacgatga ggttggtaag catgaacgct
    59641 atgatgacga taaccatcat gatcaccccg atgcccgccc ccgcaccgct gtcgcgaacc
    59701 gtggtggtgg atgcggagga tccgaagacg atctcataca tgtagttcat cggagcgacg
    59761 gtcgcgttgg agaggttcac gggactgaaa agctgccccc atacgaagaa gccaacggtg
    59821 aacaccgtcc acatgacgat gttcgtccgg aaacatcccc tgagaaacgg aagggtgatt
    59881 ttaaagaagc gctgaaagac gttcgctccc tcgatgaacg ccgcttcgat gtaatcacgg
    59941 ggaatctgct caatgctcgc catgaagatc agcatatggt agccgaccat tccgaaggaa
    60001 taggcgacca acatcgacca gaacaggttg cccggtccgg tccaaagcgt ctcgctcaaa
    60061 gccgtgaatc cgagtttcgc aaaaatgtta tggagcaatc cataggagga attatagaca
    60121 tagttgatcc acatcgttcc cattgcgatt gcggaaacga cgtttggaag atagatgacg
    60181 cttctgaaaa agcggacaaa acgcattcca ttggtcaggg aaaccgcaaa aaagagagca
    60241 acagccatca ctccaagtcc gccgaccagc cagatgttca tgatgttacg catcgcctgc
    60301 ttgaaaatgg ccgtcgcaaa gagggtgcga tagtttccca gtccattgaa acgccatgag
    60361 gagatcggat cggaaaccga ctcgaccttg aagaagctca tgatcgtagt tcgcaccgaa
    60421 gggtatagaa agaccaaaag gtagcaaagc actgccgggg cgagaaatgt gatgatcatc
    60481 gttcgatttc gtttcatatc agtaacccaa agtacatcgg ttgcccctcg tgtgaggagc
    60541 aaccgtcaga tgcatcagcg attcatcgcc tggtagaaac cttcggcatc aagctggccc
    60601 aagatcagct ttgcaaagtt ttctttaatc tttgcgttga catccgccag attctccatt
    60661 ccggccgccc acgtcatcca tttcgtagtg ttatcgatga ccgtcttggc ctcggcggtc
    60721 tgcttcggcc aagcgctgtc cttgcccatc ggaactccga gactctcctc ggcaagcttc
    60781 gcgtcccatt tgccggtggt catgtagacg atgaaatcaa acgcctcgtc ggctactttt
    60841 gagtttacgt tgatggcaaa gcactgagca ccgtagttgt tcgcttccgt tccgacaccg
    60901 gtgggcttga tcgcaggata cgcgaatgct ccccagttca aattcggggc gttacccttg
    60961 atttcgttcg gcagccaggt gccgttgagg tacattgcga cctttccggc ggcgatctct
    61021 tcaatctgcc cggcgggata gatgttggag gcggcttggc gactgatata gccctttttg
    61081 gcgaagtcgc tccagatctg tccaaactcg cggacagcgg gatctttgaa gtcattattg
    61141 tccgccatcg ccaaggtttt ctcgtatccg atcatccttg taaggttgta tccgaagaac
    61201 gcagccatgt atgcatcgtc aacggtgatc ggggtgattc cggctttgac cagcttcgca
    61261 cacacatcct caaattccgc ccaagtcttg ggaggggcgg tgatgcccgc cttcgcgaag
    61321 agatccttgt tgtacatgac tacaaatgcg aacggctgat agggggcgca cttcaacgtt
    61381 ccgccgcctt cggcccgggc aagatccagc agcgcggtcg caaccgcatc cttgtacgcc
    61441 acacccccgg tggtgggata gctcttggaa acgtactctt ccaagggaag caggtatttc
    61501 ccccatgtgg agttcacccg cgagatatcc tcatcgaaga tgtcgatcgt ctcaccggca
    61561 tcgagggcgg gttgcagagt ttttcgaatc tcacgaccgt tgaacacgac atcgatcttg
    61621 attcccttct ctttctcaaa cgcagcggcc gcttcggcta tcactaagcc ttgaggctcg
    61681 gcttcgttcc acatcgacca gtacgttaca gtcttcttgt cgctttttgc ctccgctccc
    61741 ccaccggcaa atagcggaga gaccaagagt aatgcgacaa acagaatgac aaacactttt
    61801 tgtttcatga cacactcctt ttgaattaaa aaccttgcgg tggcagccca ccacctacaa
    61861 gtatactcgc ttcccggaag atgatttatc tgaaaaaaca caatgtgtat ctgatatgcc
    61921 acaccctcca aatctatcaa aattactcct caaacccatg caagccccta tgacaagagg
    61981 atttactacg cctacaggaa gatatatact cgtggtatgg aaccaaagat taatggcttc
    62041 acaccaatcg agatgaagat tgacattgag gtaagccgct tcgtgacatt ccactatttc
    62101 gaataccccc acaagttcgt attcgagggc gaaaagcatg atttttggga gctgctctac
    62161 gttgacaagg gggtagtctt cgtggggacg gatgaggatc gccacctcct tcgccaaggg
    62221 cagattattt ttcataaacc aaacgagttt cacaccgtcg agtgtgacgg aatcatctct
    62281 cccgctttgg tggtggtctc cttcgtctcc gaaagtgaag cgatgaagtt ttttgagaac
    62341 cgggttctcc ggatcactca acgaaccaag agcttaatga gtgcgatcat cctggaagcg
    62401 aagaagagct acagcaacga ccttggcgac ccctttttca ccaagcttct gaggcgcgaa
    62461 ccaaagccgt tcggcagtga acagctgatc aagaactact ttgaggcgtt cctgatcgag
    62521 ctcatccttc aaggaggccg ggaagagacg ctgttgggaa ccactcaggc acaggatcga
    62581 aggattcgct ttgaggtggc caatgcgttt ctggagaagc acctcggcga caatatcact
    62641 ttggacgatt tgtgcaacca ttgctcgatg agcaaatccc tcgcccaaca gatctttaaa
    62701 catgagaccg gcagcagcgt catgcaatgg tatgcccgtc tgaaaatagc gaaagcgaag
    62761 caatacatcc gtgagggcaa aggcaatatg acctcaatcg cctacaaact cggcttcagt
    62821 tcgatccatc acttcagccg acaattctca aagattgaag gcaaaagccc gacggagtat
    62881 gcccagtcaa tcatcagcct tacgaacaag gatcggattc tcctctgatt caatcggttc
    62941 cccccccttt ccattgatcc gattttacgt aatactatgg tatcgcgctg ttaggcgccc
    63001 aggaggcatt cgatgcaaca taccccacat accctcatca cagtcatcgt gaatcaaggc
    63061 atggctgaag aagttatgga agctgctcga agcgttggcg caacgggggg aacgatcgtc
    63121 ccggcgcggg gaacagccaa agaggaagac gttaaattct tcggttatcc ccttgttccc
    63181 gagaaggaca tgctccttat tcttagcgga tcggaccaaa ccggaagaat tctggaagca
    63241 atcaataacc ttcctattct ctcaaaaccc ggcatcggga tagtgttttg tatcgatgtt
    63301 gaacgattca tggtgtttgg agcaccccgg aacaatctct tctaagggaa accagataga
    63361 caaaacaacc actttcatgt aaccatacac tcatgggaga actgtcaaaa cggccgattt
    63421 tcgtccgact cgctgctcct acgtggggtc gctgggtcct ccaccgcaat cgtatcaccg
    63481 gtcatggcct tgaaaagatg acaacgatag acggtccttt cttggtattg tgcaaccaca
    63541 cccataccct tgatccattc ttcatatccg cattgagccc ggtgcatatc cgctgggtgg
    63601 ccggagcgta cctctttaaa ctcccctttc ttcgaatgct tctccatcat ctggtcggag
    63661 cgatcagcaa gcaacagggg cggagcgata tgcacatgat gcgccaaatc accgccgcct
    63721 ttaaaaaagg tgacgtggtg ggaatctttc cggaaggtac ccgaacgtgg gatggcgagc
    63781 cgatgagctt tgactcgtca ttggcgaagc tgatcaagtt cttcaaagtt ccggtggtca
    63841 ttctcaactt tgaaggtctc tatgcgatca aaccgcggtg gaccgatcac cgaagaaaag
    63901 ggaatgtggt tcttgccttc aagagtctgc ttacggtcga aaaagcccag gcgatgagcg
    63961 tcaatcaact gtatgaacac ctgaagagcg agctgggctt ttcctaccga agatggcaac
    64021 gtcgggtaaa ggctcgattc atcggcaaac gctccgctga aggagtcgaa aaagccctgt
    64081 atctctgccc ttcatgcgcc aatggctcga cgatcaccag caaaggacga acgatcgcct
    64141 gtaccgcatg taacctctcc tatacactca acgagcatga acggctggag aacaacgcag
    64201 ggggcaaacc attcttcgaa gatatcgccg cctggcgtga atggcagcgt tcttacctga
    64261 ttacccatca tccatcattt cctgaagatc agggagtcct ttttcaaacc gggaacgaaa
    64321 cacatcttaa aacgatcacg accactttct ccctccgtct tgaagaagac aacatgaccc
    64381 tcacgactgc aactcaaacc tatcgctttg atttcgacca cgtcacatca atgatcatca
    64441 atgcaaagaa taccctcgag ttttactgta acgggttctt ataccgagtc cggatcgcaa
    64501 aacatctttc ggtcctcaag tatgtagaac gcttccaatc ttacgcatca acggaggcta
    64561 cacaatgaac tggaattttt tcttccatgt tggaatcatt tcattcgctc ttttactttc
    64621 agcccttatt cgagcacggg tccgtttctt tcaacgttat ctcattccct ctccgattct
    64681 cgccgggatg atgctcctga tattctacaa ctttggagcg cccaagctgg gcttgagaag
    64741 cgacttcctt ggggacatgg tctaccatct gctcaatatc tccttcattg ccatgcttct
    64801 tcgtgtgacc cccactgcaa aggtaaagga ccaagcccgg cggaccttgg ccgcaaacgt
    64861 gacggctgtc atggctcagt acggtcttca ggcgttcttc ggcttggtgg ttaccgcctt
    64921 gttgattgcc gtctatgata agaacctgtt cccggcgatc ggctataccc tgcccctggg
    64981 cttcgagctc ggacccggtc aggcgtattc aatcggaatg gggtgggagc cgatgggttt
    65041 caaaggcgct tcatcggttg ggctgacgat ggctgcaatc ggctttctcg tcggatcatt
    65101 cggcggagtg gttctcatca accaggggat caggcgcggc tggatctcca aagccgatgc
    65161 gaagcggatc gacaacaaga gtgtccgcac cggatttttc agtaggctgg agagtgaacg
    65221 accgattgga gcataccttt ccaccgatgg tgaatccctc gattctttta cctaccatat
    65281 cgcgatcgtc atgctcacct acctgatcag ctggggcttt ctgaccggtc tttcctttct
    65341 tttaggtctt atcggcccga tgggaaccga gcttgccgat tccctttggg ggatcaactt
    65401 catcttctcc gccttctgcg ctctcgcagt gaagatgttc atgaaactgg tggggatcga
    65461 aacgacaatc gataatgcga cgtgcaaccg aatcaacggt ctctccgtgg acctgaccgt
    65521 tgccgcaagc ctgggagcga tcagccttgt cgcggtaaag ggctactggg ttccgatcgt
    65581 tgcgctctcg ataacgggaa tcatcatcac ggcattcatt cttccctggt acacctcccg
    65641 cctctatgac gatcatcagt ttctccgaat gctcctgctg tacggtaccg cgacgggaac
    65701 ccttcctacc gggttggcgc tccttcgagt ggtagaccag gagttcgaga ctccggtggc
    65761 taccgattat ctctactcgg tgggaatcgt ctttatcctt gccattccct tcattttgac
    65821 gatcaatttt ccggcgtttg ccgtcacgaa gaacaacccc gtcttcttct atctcacgat
    65881 tctcatcgcc ggggtgtacc tcttcgggtc gtttgtcgcc taccttctga ttgcaaaaaa
    65941 acgatccttc atccaaccaa aaaaactctt ctatgtcgag tgatgtcggg gcgcttcttt
    66001 gataagaggc gcctctttta tccccaaagg tgacgttact ctcaaaaagg tcacatatat
    66061 tacctaaatt tctctttttg cccattgttt tgggttgttc aatcatcgtc aaaggggcta
    66121 gtatcgattg ggatgatgcg aatgagatct aaagaatcga taacatgcaa gccatatatc
    66181 atgatctttc ccgcactttt gctggcggtg ctctttagct atgttccctt tattctcacc
    66241 ttccttaaca gtgttcacgc cgtttcactc ggtggtaaac gacttgggtt cgttggcctc
    66301 gccaactatc gacggctctt tggaaacccc tcgttccatg ccagcctttc aaacacactt
    66361 cggttcaccc tcttcttcgt cccgacaaac atcatcatca cccttttggc ggcagtcctt
    66421 tccaaccgga aagggtggct ggcaaatctc aaccagatct tcttttttct gccgatggcg
    66481 gtcggaatga gcagcatgct gatgatcctg aagatgatgt tcaatccgac gatcgggatc
    66541 atcaaccgcc tcatcggtgt caacattcaa tggttcaacg atcccaatgc ggcgatggct
    66601 ctcctggtga tcgccggtgt ctttcttgat atcggttttg actttctgct cttcagtgct
    66661 gcgctgcgaa acgttcccaa tgacttgaaa gaggtagccc tgatcaacgg ggcaaacgga
    66721 tttcagatct ttcgtcatct ggaattgccg ctcatcgcgc caaccttgat cttcatcgtc
    66781 gtgacgaaca tcaagaacgc gatgctcatc agcgccccgg tgcttattct caccgaaggc
    66841 gggccgttcc gctccaccca gacgctggtc tatcagatgt acttggaagg gttcaagagc
    66901 ggaaactatg ccttgggatc ggcgatcgcc atcgtggtct tccttcttac gttcgccgct
    66961 cttctcatcc tgctccattt ggaacgaagg agaatctact accggtgaag acccttcgtg
    67021 caaccctttc aacccttctt tcccttctta tcgtattccc gatcgtgtat tcgatcagtg
    67081 cgtcgttttt ccagtacacc gatttcaccg ccctgcccgc ccgtcttttt ccctcccgat
    67141 tgaacatcgg caactatgtc cgcgcctttt ccgagtcttt cctggctcgc tttctgctga
    67201 acagcctgct caccgcaatc gccggaacgc tgctgcgcat ggctatttct attcttgccg
    67261 cctacacctt ctcctttttc accttcaaag gtcggaatgc tctcttcatt ctcatcgttg
    67321 caacgatgct ccttccctcc gatgccttga tcatcgcgaa cttcatgacg atccgggcgc
    67381 tccgacttac cgatacctat ttggggatta tcgcgacaag tttacttgct ccgactcata
    67441 ttttcatgtt gcgccagtac tttcgaatga tgagcgtgga ataccgtgaa gcggctatca
    67501 tcgagggatg ctccgattcc cgttttctgt cgatgctctt gatgccgatg agcaaagcgg
    67561 tgatcctcac ccttgcgatt cactccttct ccacgatttt caacgattat ttgtggccgc
    67621 ttctcgtgac caataaaaca tcgatgagga cggtccaggt gggtttgacg atgatgggct
    67681 tcagcgagca gctcgactac ggcccccaat ttgcggcgat caccttcctg atgaccccga
    67741 ttctcatcgt ctttatcgct cttcataaac cgattataga aagcgtatcg atacgcttta
    67801 ccgggaggta actcatgaaa cgttttatca caatagtggt tattgccctc ctcgcactac
    67861 caatgctttt tgctcaggca caggaagaga gcgcaccgta caccacgatc acgtggtggc
    67921 accccaactc cggtttggtg ggtacggcca gcgaggagct gataaaaacc ttcaaccgaa
    67981 cggttggcga agatctgaag atccgcgtcg aaggggtcta tcaggggaag gcgaacgatg
    68041 tccttaccaa acttaaaccg attcttcaat ccaaagatca gcgcgatctt cccgaccttg
    68101 cccagctgga cggctcggcg gtgctggata tccgcgattc cgcccagctc atcacgataa
    68161 acgagctggc cgggaatgag ccggagtatc tctccaaaat cgttgaagcg acgctcaact
    68221 caataaccta tcagggcaag atcctcgcta tgcccttcaa cgcatcgacg atcctgctct
    68281 actacaataa agatgcgttt gccaaagcgg gaatcgccaa accgcccaga accttggatg
    68341 atgtgaaaac agctgctgcg aaactgaggc aaaccgacga taaagggcga accgtgttct
    68401 ggggtatcgc gaacgtcccc accacgtatg agctgatggt ctggcttggc caccagaacg
    68461 gactcaccta cctcaccgat tttgagaacg gacatatcgg agccccgacc cgggtcctct
    68521 tcgatgagaa cgggacgatg gtgaacttcc tgaccaaatg gcaggcgatc tacgataccg
    68581 gatcgctggc aaacctcaca agcgatctca acggagcgtt cgcttccggc caggtggcga
    68641 tgattctcgc ttcaacgagc aacctctcca caatcgtttc gatggtcggc gaccgctttg
    68701 aagtcggcgt cgccaatatg ccgatggtcg atgaaaacgc aaccggaggg gtcaatgtcg
    68761 gtggcggagc gatctgggcg ttcgacaacg gtaaaaacaa tcaatgggcg atttgggagt
    68821 tcctcaaatt tgccaccagc gccgaagagc agctcaagtg gcatatcgcc accggctact
    68881 tcccggtgaa cgtggatacc tacgagctgc cggaatttaa aagccacctt caagagcatc
    68941 cgctcttcgg ggttgccagc cgtcagctga tggattcaaa cccgaagctc caaggcattt
    69001 gggttcccag cgcctatcag atctattatg cattccaatc gggaatcttg aagatgttgg
    69061 aggaaaagca gacgcctcag gcggcaagca aagcgatcgc taaggagatc aacggctact
    69121 tgcaggagta tcacctgatg aaggctcggt agcgcagcgg cagacatacc acctgaaata
    69181 gggacttacg tccacgatcg agatggaggg gaagatctct tccatctctt ttttcggttc
    69241 tttgatgaag ttgaagtaat cgccgccctc cgcccattcg atgatcgtgc tgacagggtg
    69301 ccgacggctg ttgtagtcgt ggatgatcac ggtctttgat gcgattcgct tcagttcccg
    69361 atagaaccga attcgatcgg gcttcttcag gccatgggcg acgtggctgg aaatgacgag
    69421 atcgaaactc ttgtccagga atggcaatcc cgataccccg tccgccagga tgaaccggat
    69481 gggaagaccg cgggtttttc ttcgggctac ggcaagcatt cctttggctg aatccactcc
    69541 ggttgttcgg attccccgtt cagccagaac ggcacacagc gctccggtgc cgcaaccgac
    69601 atcgcatgcg ctctcaaccg attccaattc actgccgtgt tcggtaagaa tcgctttgaa
    69661 ccggcgtttc tgatagtgga agaaccaacc gtagattctg ctgatccaat gaaagagcat
    69721 cttaactgtc cttgaatcga cacttctctt tgaaggtgca ctccagacat tcaaggcccg
    69781 gacactcctg gaaggatgcg gtggattcca gctgggcgat tcgccaggaa agatcctcta
    69841 cccgaccgtt gatctccgcc ctccgctcca gaacctcact gagcttgttg cggtattgac
    69901 ggagcagttc caaccgccgc cgctctccgg tgctgtcttc accggccaag cggatgatat
    69961 ccccgatctc ccgaagactg aagtccagct ccttgagttc ggtgattcga ttcaaataca
    70021 cgacttcttt gtcggtgtaa taccgctggc cgcccggaga gcgcaagaca gtcttcaaaa
    70081 gacccaacga ttcatagtgc cgaatcgttc ggaccgtaac attgcacttc tcggcgagtt
    70141 caccaattcg atagatatgc tgttccatct ccccttcatg ataccatacc aatcaactgg
    70201 aaatcaaaac ccatgacggg caaatcgaag agatggaaga agtcattgtt gaatcgggtt
    70261 tgcatttctg cttatttgca tgtttggtgt atgtatataa tacgtttcat aaaccaaatg
    70321 acatccatgt gctcaagaat gatgatatgc ccttcattct gacaaaaggc tgccaaagat
    70381 gaggtccttg gcagccgatg agcaacaacc cggtaatgaa tttagaaaat ccagttcatc
    70441 agcagcagta cgacaaaccc ggaagtggaa agaaccagta cggccccgaa ccagttccag
    70501 aacatctctt tcacatcggt gagaccggtg gtccggttga cgacccagaa atagctgtcg
    70561 tgcaggttgg tgatgccgat tgatccgata cagcaagcca atccgccgat ataggggttg
    70621 acattggcca ttgaaagaag aatcggtgca gtcaacgacc cggcggtcaa catgcctacg
    70681 gtggccgaac cgctgacgat tctcaacaga tacgccatga tgaacggaag cagaattccg
    70741 ggaatatttg cattcaccag aacattcgca atcgctgctc cggccccgct gtccttcaag
    70801 acttggccaa gagccccacc ggcaccggta acgaagacga tcacaccggc ctctctgatc
    70861 gatccgtcaa gcattgaaac aacttcttta cgggaaacct tgccagccaa tccatagatg
    70921 gcgataagca gaccgatggc gacggcgacg accggatctc cgataagacc ggcgatgctc
    70981 ttgaaggtac ccttcattcc catggcgctt gtgatggtat tcaacaagat caacaggatc
    71041 gggaatacga tgggagcgaa cgaaatgaat gccgaaggaa gcccttcagt ggagatgttg
    71101 gcagtagtag ccatctcttc cttcgtgtag gggcgatcga tcagcttgga agagtcatcg
    71161 ggatcgacca gcttgaacac ttttttcgtt cctacccatc tggcatagaa tactccggcg
    71221 accgtgatcg gaacggaagc gagcagactg atgaggatga attttccgag atcgagcttg
    71281 aagaattcaa cgaccgccaa aggtccgggg gttggaggaa ccaatgagtg ggttaccaac
    71341 agtgccgcac cgagagcaca actgatcgtt atgacgctct ttccggtatt tcgtgacaac
    71401 gactttgaca gcggcgaaag aatgacgaat gccgagtcac agaacacagg tatcgatacg
    71461 atgaatcctg ttatcgccat ggcgatctct tcacggcctt tccccagaat cctgatgaac
    71521 gtgatcgcca ttctcgtggc agccccgctc ttttcgaaga tggcacccat catgacacca
    71581 aatccgatga tgataccaat gcttcccaac gttccgccaa atccactctt gattgccgac
    71641 atcacggcgg tcggagccaa gcctgcaatc aacccggcga ccaccgaccc cataatcatg
    71701 gcaagaaaga cgtgaatttt cgtcttggtg acgaaccaga atatcattcc gatcgccacc
    71761 gccagtccga caactactcg gactacgctt acatcaccca ttgattagcc tcctctcctg
    71821 ttacatgcat atcattatgc caatgcttcg gtcaaattac cggaccgagc atataaaaat
    71881 ccattgattt tgaatatttg atggcctcgc ctttggtcac ttcaccgctg gcgacgcgga
    71941 gcatcatctc aaagacttct tcaccgactt cctcaagtga acgctcacct gtcgtgatga
    72001 gaccggcatt gatatccata tcgtgaatca tccgttcata ggtgatcggg tttccacaga
    72061 tcttgatgac cggaacgacc ggaaagcctt gcggggctcc cctgccggtg gagaaaagaa
    72121 tgatctgagc tccaccgatc gccatgccgg acatcagttc gatctcccgt cccggagaat
    72181 ctttgatcca gagacccttg cctttcggta tttcggtgta atcgatcact ccctggatgg
    72241 tcttcgtacc cgacttgacg atcgctccaa gtgatttctc ttcaatcgtg ctcaatccgc
    72301 cttttatgtt tcccggagtg ggctggcctt tgcgcatatc gacaccgatc gccttggccc
    72361 ggttttccat ttcaataacg attctgtcga tatcggcggc gacttcggca tttttcgctc
    72421 ttctcttcag aatatgttca gctccgatga attcggtcgt ctcgccgaat atgaccgttc
    72481 caccaagatc gacgacctta tccgccacat atccgatcac aggatttgat gcgattcccg
    72541 atgtcgtatc ggaagcgccg catttgatgc ccatggtgaa atgtgacatg tcgacttcaa
    72601 cccgttgctc tccggcgatc tttcgaatca gccgctgggc tttatcaagc cccgcttgaa
    72661 tcgccctgct tgtgccgcca agctcctgaa cattgatccg atcgacgggt ttgcctgttc
    72721 tacgcaacgc ggcctccaat ctgtcggtat ccacgccttc acagcccaga ctcacgataa
    72781 gaaccgctcc gacattcgga ttctctccga tctttatcag acattcggtc gttcgttcca
    72841 gatcgggagg cgtctgacaa caaccctggt ggtggatgat tccctgagtc atgacggtat
    72901 tcgccacgat cgcttcaacg acttcgttca cacacaccac cgatggcaga attgcgacat
    72961 aattgcgaac tcccaccgaa ccgtctttcc gtacatatcc aaaaaaacca gattgttttt
    73021 ccgccatcat tatacctctt tacggattcc cgctttgact ttctcagctt gcgactcgtc
    73081 ccagtcaccc cttcctctga tgctctcgat attatgaaca tgaacatgtt cacctacatg
    73141 gattggaacc gacgcaattc cgatttcctc gccatacttc gtcacctgtt caccgatacc
    73201 cacttcagcg atcgccacct tgtgaccgta ggggatatcc tgagacacca ccaattcaaa
    73261 cgatgcccct ttcttgtcga ttatctgcac gaccgctccg gcaacaaccg aatcgaagac
    73321 actggcgaca ttatcgctgt cgtgcaattt gattgctttg accatttatt gactccttat
    73381 aatgacagat actccgtcgg gtattacagt cacgtttgct gattcccctt tgagcctgaa
    73441 cgcttcttcc aatgcctgct ccacggaatg tgcatggata aagcccatcg ccctcaccgt
    73501 atcggcatca agttcagaga ccaagatgat gggatgccgt tgcagcgcct ccgcaaaacg
    73561 ctgaacgcac cactgctcgg aaacggtttt ctcgggagga gttgcaagaa tcttttccat
    73621 taattcatca ggagaccccg aacgcatgag ttttgcgaaa ttgtcaccgc ccaacccatc
    73681 cttacatgct gctaccaata tgatggccgc atgttcattt gccgttgcca gtgccgtgga
    73741 caagcctttg gggcattgat aaagattttg atccaatgga tatcctccat tggtggttat
    73801 gacgatatcc gccatgatcg gatcgattcc aatggcttcc tgaagccatg aacacccaag
    73861 ccgatgcgcc tgctcaactt ctcccgccac agctgctgaa atgtttttat cttcgtcaag
    73921 cagcacgttg aggatgaacg caagattggc cattcgggca gcggtgatca tatcctcatg
    73981 gatcggattg ccgtcaagca cgcccgtaat cgctttcgga tgcgcgattg ccatggcgct
    74041 gtgattgata ttcacgcagt cctgactggc gattcccggc aagacgctct tacgaccgcc
    74101 tgaatatccc gcaaagaaat gtggctcgat aaatccttcg gcaaccaaca gatccgtttc
    74161 tattgccaat ctgttgatct cacacttgct tcccgaagga agcacgccga gatcgatgaa
    74221 catgtccgca tcaaacgcat cgtgattgat gattcgttcc ttggcaacga gccgggaacc
    74281 gaatttcgca ttcatctcct ccttggtcat cccgcggtgc agcccggtcg ccacgatgat
    74341 cgtgatatcg gcatcgggat tcccgtccct gatctcttcc aacagcaaag ggagggtgat
    74401 cgcgctcgga accgagcggg tatgatcgct ggtgatgatc gtgattcggt ttttccctcg
    74461 gaccaagtca cgaagacgat cgctgcctac aggatgcgca agcgcttccc tcaccaattc
    74521 agaaccgctt cttgcagcat cattctgctt cttggccgga ggaggaacaa gtacaccggc
    74581 cagcctttga tcatcaatat ccagccggat tttttccaaa tcatagggta attcaatcgt
    74641 cgttttcatg tcgtccttca gcacatgctg tacaatatac tatgtataca ttatgcttgc
    74701 tgtatgtggt tgtcaacttt tttctacacc ggtttttcga aaggaggacg tatccgtccc
    74761 taattaatcc ataaccgtac tggaaatatt gaaaaatcag ggtaattcgg gtaacgtgtc
    74821 agcatgtcga taggtacgaa taaaaaggtc atcttgacga taaaagatca ggtatatcaa
    74881 atcatcagag acagcatcat ctccgagcag ctcaagcccg gcgatcgagt caatgaagtc
    74941 caaatcgcaa aggacctcaa tgtcagccgt tcacctgtac gaagcgcgat caatgagtta
    75001 atcggggaag gattgctgga aagcgtcccc aacaagcacg taaaagtgag gaagctcact
    75061 gaaaaagaga tcctcgacat ctttgaactg cgtttgcttg tcgaagggta tgcggtggaa
    75121 aaggcgatcg aacggcttga tgatcggatg aaagccacgt tccgtgattt cagggatcgt
    75181 ctcaaagcat gcaacaccta tgcaatgctt ggcaagtatg tggaaatcga taccgagctc
    75241 cacgatttcc tcatcaaggc ttcagggaat gaaatgttgc atgaaacgat gcaccgagtc
    75301 gcgctgctta tcaatccctt ccgagtggtc tcattgaaaa gtgaaacacg attcattgaa
    75361 tccatcgatg agcacatcgg gctgattgat gctctttgtg aaaaagatct gaaaagagcc
    75421 aaggaacgaa ttgaaactca cttgacgttg gcaaaaaacg aagtcgtgga atacctcaag
    75481 acccggcaca attgacccca agcccatgat caacctcttc cgaaacaatc aggtctcgga
    75541 agaggagaca ttgacaaatt ccatggatac aaggaaccaa tgtcattcgt ccgaaaaaat
    75601 tgtcatatat ccatcctttg tagcttcata gatggcttcc agatccttct tgacgaagcc
    75661 gatatcccac gtgtcgctgg tgtagtgggc gcaggtgcca cacgaagagg ataacgctct
    75721 tggtaccgga gcgagcttgc attcgatatg atgctttctt gcaaacttct ggaattgcac
    75781 cgcaccatat tggctgtaga aggttgcgat aaaccgggtc attgtttctt cagatccagt
    75841 ttccatccat attcggtctc gcttgcaacc acctcgtact tcatcgcttt agcaaatctg
    75901 ctgacatttt ccttggctgc gatattgtcc acgagcaccg aaagacttgt cactttttta
    75961 tcaatcacct tcttcgtctc tatgatcggt tgaggacagg aaaggccccg agcatcaatc
    76021 tccatatcat gcctcctttt tcttagcttg gatgatgacg aacgcaatcg tcgtgattac
    76081 gacgaaaccg agtatgacgg cgatcctgcc ggcgaccgtg gtgccttttc ccgaagatgc
    76141 cagaccgaaa ttatgggaga tggcggcccc tgcgagcatg cccagcacgc tcatgacgct
    76201 gtcgacgttc ccctccccgg ccaggatcgt ctgcctgagc ggacatccgc cgagcagcac
    76261 ggaaccatac ccgaccagcg ccatgccgag gaagttccag agccaatcgg tatgggcgac
    76321 ggcctgaccg gtgaaccccg gcgtgaagtt gcccgtcacc aagttcatga ccaaggcgga
    76381 aacgaacacc agaaccaagc cagtcagcag ggtcgaatcc cggaacagga acacgtcacg
    76441 aacgcctccg gccatgcaga agcggttccg ctgggcaagc gcacccacga cgagcccggc
    76501 gacgagggag atgatgatcg gcgcccgcat gcttccgggt cccgattcgc tgaatttcaa
    76561 gagcgacgga atgaacagca acacgacaag gagccctagg aaaagtccgc tgatcatccc
    76621 tccctccacc ttggactgct tatagcttct tccaagggtg aaccctttgt tcaacaacaa
    76681 tgatcccggg atgatgccgg cgatgaagcc aaagagggct accaatgcat tcagatctcc
    76741 tccggcaagc ctcaggacca tcctgaacgg acagccgagg aagaccagcg ccccgatcat
    76801 cacaaaagcg gagaagacga atcgcagtac cgggctcgat cctcctctgg gtttgaattc
    76861 cttttttaca agactgatga ggaatgcccc aaggagtaat ccgatgatct ccggccgtac
    76921 atactgcacc accgcagcgc tgtgcaggga gagagctccg gcgatatccc gatagaaaca
    76981 ggcgatgcag aaccccatgt tcgtgggatt cccgagcagg acaagaatca cggatatgat
    77041 tcccgtgatc gctccggcga tgatgagcga ttgcttctct tttgaaatct tcatatggcc
    77101 tccaaggttt ccacattata accattattt atccaaatat acaatgggaa ttttcattcc
    77161 gttatgctag cattgagaaa tgaacacgat ctatctggat aatggggcca cctcttggcc
    77221 caaggctccg accgtagcga gcacaatggc atcattcatc actgaagagg cggtcaacat
    77281 cggcaggggc ggctatgacc gggcctacga gacggccggt cggatcgatg agtgcagaga
    77341 aaagctgggg gccttcttcg gagctccgga ggaccgcaac gtcaccttca gcctcaatgt
    77401 cacccatgcg ctcaataccc tgattgcagg cctgttcacg aaagatgacc atgtcctggt
    77461 gagcgggatg gaacacaatg cggtgatgag gccgctctct catctcgata tcccctattc
    77521 gatcatcccg tgcgaccggg aaggcagaat ccaccttgag caaatccccc atctcatcaa
    77581 cggaaaaacg aaagcgatca tcatgacgca ggcgtcgaat gtaaccggca cgattcaacc
    77641 aataaaagaa ctctcttcaa ttgccgggaa atacggactg ttgctcctca tcgatgccgc
    77701 ccagctgccg ttatccaatc ccctttcgat catcaaagac aatcttgatg cgatcgcgtt
    77761 caccgggcac aagagcctcc tcggtccaca gggcgtcggc ggattggtct tatccgaccg
    77821 attggcgaag gaggtcaaac cctgggcgtg cggaggcacc ggcagcaaaa gcgacctgct
    77881 tgagatgccc gatttccttc ccgacaagtt cgaggcggga acccagaacc tgccgggaat
    77941 catcggcttg tccgcagcgc tggactttat caaggacaac agcgagtcga tcatcaaaaa
    78001 cgaatacgaa accaccgagc ttctgctttc ctatttcctc tcagacaaac gcatacaagt
    78061 gataggtccg aaggacatga aggatcgcac gagcgtgatc agcgtcgact ttccaacctt
    78121 ggacaatgcc cggatcgcct atgagctgtt ccttgatgcc ggcatcgaga cgcgagtggg
    78181 cctccactgc gcccccgtcg cccataaaac aataggcacc ttcccccaag gcaccctccg
    78241 cttcagtccc ggatatgcga ccacaaaaga ggagatcctc aggacgatcg aatcctgcaa
    78301 aagagtgttg gacaggttat aaacggaaga cactgctctc ggatagtata agatcggaaa
    78361 gcatttaaag agaaatcata gcagccgttc ttcatgttaa ttaataaaac gggatgtatg
    78421 acgaatctaa caaaagtggt attcacttct ttcttatatg aagctctctt cagggcacag
    78481 cgattacatc atactcgcac aaactacaag aagaaacaaa accgcaacta taatagcgac
    78541 aatcaagaga acgattcttg ctgccgtctg cccggcacta ggagtatttg ttacccgagt
    78601 ctcaacggtt gtctttccgg tttcctgatc agttgtagta attgtctctt ccttcttatt
    78661 ggatttgaac gctaatacca atatgagaac gacaacaaaa gctatgataa accacataat
    78721 ctcatccttg tgtttgataa ccatgaagag tacatcaagt tgcttgatac aatcatatca
    78781 catctgccta ataatttaag ttttttgttt ctaatttaaa atctatgaac tctttatcca
    78841 tggagccagt tcatccatga accgttccgg cagtccgctc tgaacacttt tacctatatt
    78901 gacatttgtc tggtggaatg ggtatcatga agtaaaatgt tagccaaggc taataaacag
    78961 gggtattgtc ttgatttcgg tacacggtct gtctgtcgga tatggaaagc gcaccatctt
    79021 gcacgatgtc gatttcagcg ctgaatcatc ccaaatcatc gctctgatcg gacccaacgg
    79081 ctgtggaaaa acaacgatgc tgcggaccct gagcggacaa atcaaacccc agaaaggcag
    79141 cgtgagcatc tgcggacagg aactatcatc tctgaggcaa aaggagctgt ccaagtgcgt
    79201 agccttcctg ccccagagac atgaagtcgt tcacggcgtt tccgtacatg agctggtcgc
    79261 aatgggaaga gccccctatc atcgaagcgg ctggatcctc tcgatgaagg acaaggagaa
    79321 gatcaaatgg gccctctcct acatgaatct ggaagagtac gtgtcgcggc ctgtggacca
    79381 gctttcaggc ggcgagcagc aacgggtttg gatcgccatg gcgcttgccc aggacacccc
    79441 gatcatcctt ctggatgaac cggtcacgta catggatctg agattccagc gggagctcct
    79501 gagcaccatc gccgacctga gggacaccca cggaaagacg atcgtcagcg tctttcatga
    79561 catcaaccat gcgatcgaag tctccgatca tatctacctc ctcaaggatg gccgggtcca
    79621 tgcccagggg acgaacgaag aggtggtcac ggaagacaac atcggtgatg tctacggcat
    79681 ccacgcccat gtctgcaggt tcaaccgatg caggcgcaac gtggtggtgc cctcatccct
    79741 gaatcgaggc tttgaacgga aacacgcaca tcgggtcgtg atgtagattg aagcatatgc
    79801 acaaaacgaa caaggagatc gtgtaatgag aaatatcgtt gtcctattca tagtgacggg
    79861 catgctggtc atgcctctct tcgcccaagg cgcaaaggag cagcctgtcc ggaccgaggt
    79921 cgagacgaag gtcgtcatcg atgagctctc ccgttccgtg gcgattcccc aaaaccccca
    79981 gcgggtcgtg gccctcacct ccgcggtgat gcagtcgctg tacaacatcg gaattccacc
    80041 ggtgggcaag gtggatgagt accgggtgac aaaggaaggg atggccctgc cttcggtcgg
    80101 caagacctcc gcgatcaaca tcgaagcggt gtatgcattg aatcccgatc tcatcatcgc
    80161 cagcagcagg ttccattcgg cgttgaagga agccctcgag caaagcggag ccgctgtgta
    80221 tttcttcgat ccggacaagg tgggagaaat ctccgtcgtg gatctgaaca tttaccttgg
    80281 gaagctgctg gacagggaga aggaaggcag gaaatacgtc gagtcgatct actccctcgc
    80341 ccgggatctg agaaccgata tcgtcgagcc tttgggaatc ggaagtgcgg tgatgatcaa
    80401 agacggtgat accataacgg ccgcccagag cgcctcctcc tatggctcca tgctcaagct
    80461 gctcggtctt cggaacatcg ttcccgacaa ccttcccgga tccggcagag gttccttcgt
    80521 gaagtacagc atcgagacga ttctggagcg acaccccgat gtggtcttca tcctcgcatc
    80581 gagcaacgat gaggcgagca accgggcgat gcttgagaag ttcaggaacg acgagcaatg
    80641 gaaacactat acgttgcaag gtggacggtt cgtgctcctt cccttcctgg cgaatcccaa
    80701 ccgaacctcc gtcgagaaca tgctcaggat caccgcggat gcgttgaaga agaacgactg
    80761 aattccccta tgaagcgttt tcactcccat cggctcggag tgcttggtgc ggcggcgatt
    80821 cttctggtgt tcatcatcgc cttgagtctt tccgtcggct ccgtgcaggt gtcccttgga
    80881 aagatcgtcg gcatcctctt cggaaagaag ggcgagggac tgcctgtcca gatcgtgagg
    80941 aacatccggc ttccgagaac ccttgtcggt ctgctggtcg gcatgaacct cgccgtttcg
    81001 ggagttctgc tacaggggat catccgcaat ccgatggcct cgccgaacat catcggcgtc
    81061 aatgccggcg ccggtctggc ggcggtcgtg gtgatggcgc tgtttccaag tcgcatcggc
    81121 ttcatcccgc cggcctcgtt catcggggcc ttgggagcgg ccctgacgat cagcgccctc
    81181 gcgaactcga agggagggaa gagaagcacc gtctacatcg tcctggccgg cgttgcggtg
    81241 tcgaacctcc tgaacgcggg aacctccgcg ttgatggtgg tctacagcga catcctcgag
    81301 gtgtcctact cctggcttct cgggagcctc tcgggaagaa gctggtcgtc attcgccctg
    81361 atctggccgt acagtctggc cgggctcgcg ttcgcgttgt tcctgagtcc gaagatcaat
    81421 ctcttcagtc tgggtgatga cgtcgcttcc agtgtgggac tttccgttcg gttgtatcgg
    81481 tttctcgccc tgctgctcgc tgccgtgctt gcaggaagcg ccgtcagcgt gacgggaacg
    81541 atcgggttcg tcggtctgat cgccccgcat gcggccagac tgctggtggg aaacgaccac
    81601 cgctacgccc tgccgctctc tgcgctgctg ggcgccattc tgctgattct ttccgacacg
    81661 tttgcccgga cactgttctc cccggtggag ctttcggtgg ggatcatcac ctccctcttg
    81721 ggagccccgt tcttcctgtt cctgctcttc aggaaaagaa aagcctcggt tctctagatc
    81781 aggcgaacag aagaaggctg atcgccatca cgagcattcc tgcgatgagt cctccgatcg
    81841 cgatgtggtg ttctccgaac tcctccgcag tcggcaacag ctcgtccagc gagatataca
    81901 ccatgatgcc cgcgactccg gcaaagacga tcccgaaagt cgaatcgctg atgaaattct
    81961 tcaggatgaa gtagccgacc agcgctccca gcggttcggc gatcccggag gcgaaggaga
    82021 gccagaacgc cttcttcctg ctcttcgtcg cataatagat cggaacggac accgccatgc
    82081 cttcaggaat gttgtggatc gctatggcga ctgcgatgct gatgcccagc gtgggatcgg
    82141 aaagcgctcc catgaaggtg gcaagtcctt ccggaaagtt gtggatggcg atcgccaatg
    82201 ccgagaacac ccccagcctg aggagccgct gctcggatgc cttgtcgacc ggttcgttga
    82261 tgtttccgat ctcatgaggg ttctcatagg aaggaatcag ccggtcaatg atggcaatca
    82321 gcgcgattcc gccaaagaag gcgatcgttg tgacccagta gccctttgtc tctccgaacg
    82381 ggatgctcag cgagtcccgg gccttcacga aaatctcgac gaaggagacg tagatcatca
    82441 cccctgcgga aaatcccaac gccccggcaa gaaacttggg attgaatttc ttggagagga
    82501 aagccaacag gctcccgact ccggtagcaa gaccggcaaa taacgtcaga ctgaacgcga
    82561 acaatacatt atccatacca tctccaacac ttcggcaaac gtatatacta ccctatcatg
    82621 ttcaaacgaa tcttcctact caatcccaat aagttagctt atgctaactt attgatcatg
    82681 taaagacctc tcccttgaga cgaaatgtga gtgttcgatg caacgtaagc gatcaatagt
    82741 ccggatatgg aatatataaa tcgcaaacca tataaatgta aagtacaatt aattaagtaa
    82801 tctcatacgt atttcaggag tcatacatgc gaatagctgt cgcaggaata gggtatgttg
    82861 gtttgtcaaa ttcggtgctg ctgagccagc acaatgaggt ggtggccgtt gaccttctac
    82921 aatccaaggt tgatatgatc aacaagcgca tatctcccat cgtcgatgct gagatcagcg
    82981 aataccttgc acatcatgat ctgaagctga aggcgaccac cgacggggat agcgcctatc
    83041 gggatgccga ctatgtgatc atcagcaccc cgaccaatta tgatcccgac aagaacttct
    83101 tcgacacttc ctcggtagaa gcggtgatcc ggcaggtgat ggcggtcaat ccgtcagcca
    83161 tcatggtgat caagtccacg gttcccgtcg gatttacaac cgaagtgagg aagaagtacg
    83221 gatcggagaa tatccttttc agccccgagt ttcttcgtga gggacaggca ttgaaggaca
    83281 acctgtatcc ttcccgcatc atcgtagggt gtcccttgga tgatgcaagg ttgaaggaag
    83341 cggctcacac gttcgcctcg ttgctggcgg aaggagcgat caggaaagac atacccatac
    83401 tgatcatgaa tctcaccgaa gccgaggcgg tcaagctttt tgcgaacacc tacttggctt
    83461 tgcgcgtctc attcttcaac gaactggata cctatgcgga gattagagag ttggacacaa
    83521 aatcgatcat cgaaggaatc gggctggatc ctcgtatcgg aaatcattac aacaacccgt
    83581 ccttcggtta cggcggttac tgccttccca aggacaccaa gcagctgttg gccaattacg
    83641 agagtgttcc ccagaacatc atgactgcga tcgttgatgc taacaggact cgtaaagatt
    83701 tcatcgctga gcgaattttg cagaaagtag gttttgcttc cggtatcgtg gtgggcatct
    83761 accgtctgac catgaagacc ggatcggaca acttccggaa ctcctcgatt caaggagtga
    83821 tgaagcgcat caaggcgaag ggagtcaggg tggtggtcta tgagcctgct ctcaacgagg
    83881 acgagttctt cggcagcagg gtctgcaaaa atctggatga gttcaagcaa ctgtcaacta
    83941 tcatagtaac caacaggtat acacaagaac ttgaggatgt gagggacaag atatacacac
    84001 gcgacctgta tgggagggat tgaataacaa acattcacat gaatatcaga aaaacggaat
    84061 gttcagacga ttcaatatgt ccatcagggc tttatgggct tgtttgaaca gctcttctcc
    84121 ggaaaaaccg cgattgggtc cggcgtaggc aagatggggc tccaaagaaa gaaccaagtc
    84181 cttgttccgt acggcgttca ggattctttc cagctcgaca tcgccatctc cgggaagcac
    84241 gcgtcggggt gaattgaatt tggcgtcttt gatatgcatg tactcgatat atggcattaa
    84301 tagcggcaga caatcggtaa agggttgttc gcctgctccg acgaaagccg ctccatcgaa
    84361 tgcacatctc aaatgtgcgc tatcgactgc ttcaaacagt tgtcgtgaga cttttcccgt
    84421 ctgacccacc gtgttgtggc caccttccat gaccaagacg gtgtctgtcc tttttgcccg
    84481 atcagccatg agcgataatt tttccgaaga aagcccgatc gcttcatcaa tcgtcatatc
    84541 ttgttcgttg aagaaggaga agattctgat atactcggtt ccgagcatat gcgcgatttc
    84601 cgatgctttt tcaacctgct ccacgacgat cgaaggtgaa atggaaacgg gttgtttgcc
    84661 gatcggagac gatacgcagg tgatggtgat tccccgatcg tcggcctgct tccttatttc
    84721 gcgaagcgtg cgttccggaa gatccatcac attgacatca tcgacggtcc ggacttccat
    84781 gcccgcaagt cccagctccc gcatcactct gaactgatcg cggagagccg gtgctatctc
    84841 gtcggcaaaa caactgattt ttatgttctg tctcctcttt gaaaagagag cgagagctgc
    84901 ccgtatcgct acgggcggct tgagaatttc aatttacaga gccttattcc attgctcgga
    84961 agaaatgtgg acgatatgtt ccgtatcggc agatgcatgg atggcatcgc acaccgtggt
    85021 aaggttgcgc gtgaacgtaa gcggaacccg atattcgcat ttgtggcgaa tcacctcggc
    85081 aatatgggaa taaatcgcga atcgatcgcc gttatactga ccgggcagcg gataggtttt
    85141 gttatcgtct tccgttttgc tccgatatgc cgtcgggtcg atcggtcccg gataactgat
    85201 cttatgcaca tcgatggttc cgtcggttac gaaaatcgtt cctttactgc cctgaacgat
    85261 ccaatgggga agcttgtcca ccatgatgca gtgctcgcag ttgagaatga ttccgctttg
    85321 ggtcttcatc atcgcgacga aggtatcctc acagtccccg tccatgatca cctgtctttt
    85381 ttccgcgaag acctgcacaa ccggttcgtc gaccaatgcg agcagctggc cgagaagatg
    85441 gggaccccag ttgttgaggt agccgccacc gaattcctta tagatctgcc agtcgtagcg
    85501 ctttccgaat gttccggcgc gtcggcggat catgtacact tgtccgatgt caccggagtc
    85561 gatgatttcc ttgatggtac tcaagtcggt gccccagttc tgcggcagga acggcatgag
    85621 gcttaccccg tatttggcct tggctttctt ggcttcatcg atgatgaacg ccgcatcttt
    85681 cgaatcgagc gcccaaggct tggtaatcaa gacgttcttg cctgcgcgca gacagtcggc
    85741 agcgaccttc gtatgagaaa cgctgtcggt gaggacgacg atatattcga cgtcggtagc
    85801 gagcagcgct tcgctacttt cgtatgcttt gcagtctgtg tgaccgttca gatagaaaac
    85861 ctttgcaagc ttttcagctc tgtcgtacac gaggtcatag gtgcctgtta cgatgaattc
    85921 cggatgtctg agcaagatcg gcgtatgaag ttcattcgcg tttttcccgc atccgataat
    85981 ggctgtctta atgcgtgcca tgatgtccct cccttatgaa caaaattgtc gttttaactg
    86041 tgtcagtgcg tttttcaggc tgtccgggcc tgtaccgcct tttgattcaa atgaaatggt
    86101 ttcgtcatat ccgagctctt tcagaatctt cagcgctttt tgaaggaatt cgctgaatcc
    86161 atcttccgga agcatgcggt ccatctcgca aatatgcacg tgatgaagca tggggcggac
    86221 atccctgaga tcttccgggt tctcttcgtt gtactgcatg tgcatgaggt cggcgagcag
    86281 ccggatctcc ggcttgttga ttcgattcac gaaatattcg ccgtccttca ccgtcagaag
    86341 gatgttgccg gattgtttgc gtataggctc catcacgatc ataatgccgt acttccgaca
    86401 atacgggatg atcaactctt cgatgacctc gcacatctga tcataccccc gctcaagacc
    86461 ccagccgtcg gggatgtttc ttgaattgtt gcttccgaat acgatctttt tcactcccat
    86521 gcgttgtgca cgacccatcg ccttgtccag atacgcgtcg atttcggctt tgtttaccga
    86581 cggtccggta accttgatgc ggccggggaa aaaattgcag ctgcagtcac aggcgagacc
    86641 aagacgcttg agctccgcca aaagcacttc gaaatcatca tcgctgagcg tttcgatcat
    86701 catcagcgga aattcgacga aatcgaatcc tgcttccttt atacgcttga ggagctcgta
    86761 ctccaccctg tcgcgtgtcg ccgtcgcgat atccgtacac attccgtaac gcatggtaaa
    86821 ccctcctaat aacaaatcaa tcaaacgata tacccgacag ctctcggcag ccacatcgta
    86881 aggggcggcc agaacgtgat gagcaccagc gcaaccgtca tgaatgcaag ctggggcagc
    86941 aaggctttcg tcaatcgctc catgcttatg tttcccaccg agcagccaac gaacagaacc
    87001 tgcccgaccg gcggggtaag cagtccgaca ccgaggttca gaatcaacat gatgccgaat
    87061 tgaaccgggt gcatgccgag cttggtaatt accggcagta aaatcggaac catgatatag
    87121 atgatggaaa ccatattcat gaacatcccc atgatcaaca agattccgtt gatgatgagg
    87181 agcatcaaga ccttgttttc cgttattgaa aacagaccgg cggttacgat ctgcggaatt
    87241 ctcaagtacg tgatgaccca gctgaacgcg gttgcacagg ctgcgagaat caggaccgaa
    87301 ccgatggtct tgatcgtgtt tgaaaaagct tcgtagagat cccttctcgt tccggcacgt
    87361 tcgatcacga aggtgaagat gaagcagtag acacacgccg cggctgcgga ttcggtagcg
    87421 gtcatccatc cgaagagcac tcctccgacg accactatca tacaaccgat ggccggaagc
    87481 gatttccaaa gcgctctgac gaaattactc catttgaacg gttcggcaac ggggtacttg
    87541 cgtcttttcg aaacaatgaa acaatagacc gcaagacaaa gtccgagcat gatcccgctg
    87601 agatacccac ccatatagag tgcagcgatc gatacgcctc cggcggtgac tgcatagatc
    87661 accagattat gactcggcgg gatgatgatt ccctgaattg atgaagtgca ggtgagcgcc
    87721 actgaaaaat cttcatcata tcctgcttct ttcatcagcc tgatgacgat tgacccgatt
    87781 gagctgacat cggctaccgc agatccggat atgcctccga aaaacatcga gtcgacgacg
    87841 ttcaccatcg caagaccgcc cggcatccag ccgaccagaa cgtttgcaag tcctactatg
    87901 ttggtcgcca tcgatcctat gctcatgaac tctcccatca gcatgaaaca aggaaccgcc
    87961 agaagcgagt agttggccat tttgtagaag atagcctgaa acaccaccag caaattgata
    88021 tccatgaata aacaggtgaa aacggcagca agaccgagtg agtaggcaat ttccactccc
    88081 atgagcaaca tgaagatgaa agaaccgctc agaacaataa ttcccatcgt cattgtgttc
    88141 attcaaacac ctccagttct tcagcatcat gttcatgacg atcagtaaat atccttttcg
    88201 tatcgaacaa aaggaaaagc aggcaaaaca ctccggtaag cagacccggc agaacctccc
    88261 acctgaacga taggttgatc gcgccgaaac gttgttttgt ggtaataaag atcaacaatc
    88321 cgtagtaggt catcagcatg aaaaacacca gtttgagaaa atatcggatg attccaaagg
    88381 cccgcaacag cttgggggga aacatggtat cgataaacga aagacggaca tgagtgcgat
    88441 ggtattctgc aatacacgaa ctgaataaac cgacccagac aagaagaaat aaagagaatt
    88501 cttcagacca tgacggggta acattaaaga agtaccggcc tattatcaca atcagcaata
    88561 caactatgat gagaaacaaa gctacgatgc atatccagtt ggcaacattg taaaccgctt
    88621 tcctaatctt ttctaacata tggtttcatc ctctatatca gtcccaatca caccctcttc
    88681 tcgtctgaag agtatgtcat tatcaaagca ggccttgaga atatccgaat cgatatcttc
    88741 tgaaccgaat gccgctaacc cattaaccgc ctaccgtact ttctaccaat aagacccggt
    88801 catggatatg tgtataaaca ccgcggcata cgcttaaacc aacattgcac gtctggttgt
    88861 catcaaacaa agtgcattcg attgggggat ggcgattaca ccaccccccc aatcatcaat
    88921 cctacttgta actctgaatc gtgctgatga aatccggtct cacattggat gcatattttg
    88981 cataaagacc tttacaggaa tcgagccacg cctgtttgtc ggtacacggc gtaaacgtga
    89041 cacccttgcc ggtgagaaaa gccttgttct ctgcaatctg tttttcagtt tccgcctgga
    89101 accagtcgca ggcttccttg agcgatgcgg tcaacatctc ctgttcagcc ttggtgagct
    89161 tgttccagag tgcttcgttc atgagaaata cggaacttcc gacgacgtgt tcggtttcaa
    89221 gaatatactt tacctgttcg taccagttgt tgtttctcat tcctgaaaga gtgttttcac
    89281 aaccgtcgag aacacccgtc gacaaagcag tatagatctc actgaatgca agcggagtcg
    89341 cacttgccca gcagttgatc atgtcaatct tgatatcaga cgtttcggaa cggatcttca
    89401 gctttttgaa atcaggcaga gtattggccg gtttcttggt gaacatattg cgcgccggag
    89461 tacagatgta attataaaga gaacggatgg ttccatctga tttctccaaa accagatcgc
    89521 ccaagctttt accataatca ctgtacagga agtccaatcc accctgtaca ctctggaaca
    89581 ggaacggaag accgagggcc gtgtattcgg gaatcttgat tcctcggttt gccagattgg
    89641 ccgggttgat gcggatgatc gatacggtac ccagccgaag catctccatg gtgtccgctt
    89701 ccccgccaag ctgtcctgaa ccgaagacat tcacttcgat gcgaccgttg gatctttctt
    89761 ccacaagttc tccaaacttt acaagcatct gacaggcgag atccgaatca gcattgattt
    89821 caggaatgga catcgagatc ttttctgtgg ctgccgcttc ttttgctccg gcagcgaaca
    89881 gcatggcaaa tgttgccagc accacgaata agagcgctac gactttcttc attgtaatac
    89941 ctccaatttg ttacaaaata agttgtaaac tctgtgtcga atctccatat atttcgaggc
    90001 taacattggc attagcgttt cacaatacaa atttaggctt actttttatc ggtttttttg
    90061 tggtatggcg agctgtgtcg ccccatgaac tcaatcaaac cgggaaacac cttatgacgc
    90121 atttcactgt cgtcatcatc ttgaattatc aatgagctca ctagaatctt ttgtaagtgc
    90181 atcgagcgag acatgcagcc tgaacagatc cgcaaattcg gttttggtgg aaacccctgc
    90241 gtttttatag agctttttca atcggtaatt gatggtaccc ggagcgaccg cgagctcttc
    90301 ggccaacttt tcgttgctat catttttcat cactccggta ataatcatca tatcgagcga
    90361 atcgcactgg gcaagacaac aatggatcgc ccgaatggcg ttgatgccat ttgcaacgca
    90421 gggggcacaa ccgtcatact cggattcaaa gtcattttgt ccggtttcgt catcttgctc
    90481 gactaccgga agaaatcccg tactgccgcg cggtttgatc gtactgcgca tcgtcgtcac
    90541 gatcgattcg acattgggat tctgattgag cgttttccag atatcgaaca cctgttttcc
    90601 cgcttcataa taggaacggg tcgccgacgt cagagacggg catgtataac ggcagagtga
    90661 gatatcgcca aggcccgcca cgaacagctg ctcgggaatc ttgatgttgt ggtctctggc
    90721 atagttgatg atgtacgccg ccgaaaaatc gttcgaacaa atcaccccgt catacatttc
    90781 catgcgcatg agaaaatgtt ccgtgggatt gtgcgaatcg acatcacgga agtacacgtc
    90841 ggcatacgag gtattcatgt tcaattgcct agaggtcatc agaaaacttt cgaatttggt
    90901 aacgtcgttg ctgctgttgg aattgatatc gaccaatgcg atgcgtcttc ggttatggta
    90961 atggaaatag cgcaccatcg cctcaatggt cgatctgctg ccgtacatcg taccgctgat
    91021 atcctctccg tagttgccgg gaacggcacc gatgaggatg atcctgaaag cgcgtcttct
    91081 tgcagccgag atggtttccc gtatccagtt ttttgtggtg ccgagaatga cgatcgcatt
    91141 tgagctgggc tctatatcct tcaccgacgc cacttgcttt acggtatatt tttgcttcgt
    91201 tgcaacgctt tccaagcctt ctatgcttct tgcaaaccag aatgggatta tataacttgc
    91261 ttctacgaga agaaatatag tcgtcttgcc atctgctgaa gttgtcatgt tcctccttag
    91321 aagctcattc gtcaaagcta ccgacacaac ccgaattata gcatgcatac aaaaaatcac
    91381 aaaatattta gcccgttttt tgtattgttc cctatttttc ttcccaatat gatggaaatg
    91441 cctgacaaga tattttcagg tcttcaagga gagaccacgt gaccgctttc atcacaaata
    91501 tacagagatt ctcgcttaac gacggacccg gaatccgtac gaccatattc ttccaaggtt
    91561 gcaacatgga ttgctcatgg tgccacaatc ccgaaacgct cccccatcgg ccggtattga
    91621 tgttctatga gaacaagtgc atcggatgcg gaaaatgttt cgaggtatgc cccgaaggag
    91681 cccacagaat cgtggacggg aaccatgtcg tcgaccgtac gttatgcact tcatgcggaa
    91741 catgcaccga atcgtgtttc gcgggtgctc tcgtcatgag cagccggaaa tataccgtcg
    91801 aagaagtcat gaatgaaatc cgtcaggaca agctctatta cgatctgtcg gaaggaggtg
    91861 ttaccttttc cggcggcgaa gccactttgc acatggcttt tgtcgaagct ttggcagatg
    91921 cctgccatga agaaggcatc aaagtagcga tagaaacgaa tatggcgatc cccttttatc
    91981 atatagagag catgctgaaa aagatggatc tcatcatgtg cgatctaaag catgtcaata
    92041 cttccatcca caagcagttt accggcctcg aaaatcagat gattttcaac aacgtcggtc
    92101 gtgcaagcct gctcggcatt ccgatgatcg tacgtactcc gttgattccc ggagcaaccg
    92161 actcgattga aaatctccat gcaatcgctg aatttgtttc gaagctgaag aacatcctct
    92221 actacgaact gctgaatttc aatcctttgg gaggctcaaa acgaatcgcc atcgacgccg
    92281 aggattcttt cgaagcatgc agaccgttct cggacaagca actcgaagag atcaaagcca
    92341 agcttgagga taccggagtc agcatcaaag tcagctgaag gagcattatc atgagcaaga
    92401 ttcattatat aaacgaattt gacgaagaca gaacattcac cttcgacgaa cggatagctc
    92461 tattgagaaa gcgcaagata gtccagactg aagaaaaagc caaacaaggc ggagccgatg
    92521 aggacgacta cggtctcatc gttcaggacg agttcgtcta ccaactgaaa ccgaaccatc
    92581 cgaacggatc gatatacggg tatcgcgcat ggacggaaaa ctattgttcg atccttgcag
    92641 aacatcccat atatgtcgat gcgctcgatg cgttcagcgc aaagggattc ttctttcttg
    92701 agcggctcag accgaaaacc aaaaaatgga accccgatta tccgtacgac gatcttcaaa
    92761 agatattcga ccgctaccag atcatcagcg gcatcgacaa ctgccatcat tttactcccg
    92821 acatccaaat cggactcgat ctcggttggg gcgggatctt gcggaaactg aaagaacaaa
    92881 gagcgttgca cgatgaaacg catcatgaat tctatgattc ggaaatcgcg gtcgtcgagc
    92941 acatcatcgc attcatccgc cgtgcaggag atgagattga agccttggca tgtcaggaaa
    93001 agaacagaca gcttgccgaa aacctgcata cgatggccgc gatcaatcac aggcttgcca
    93061 ccgaaccgcc gaaaacgttc cgcgaggcca ttcaatggaa caattggttc agcatgctga
    93121 gccgtacata caaccgggga agttcaggcg gacagatcga ggatctgttc aatcccttct
    93181 atgaacggga cgtcgcttca ggaatcctga ctgatgaaga ggcgatattc tacctcgcct
    93241 gcatgtttct gcaggacagc cggtattggc agctcagcgg acccgatgag cacgacaatg
    93301 ataaaacatg tcatttgagc tatctggctc tcgacgctgc ggacaagata aatattgcga
    93361 cgaacctcac catccgagtc cacgacaagc tcgatccggc gtttttcgag aagtcggtcg
    93421 agtacctgtt taaaaacaag caaggctggc cgcgttattc aagcgacaag gcgttgatgg
    93481 aaggcttcat gcgttgcgga tacagcaaag aacttgcacg caagcgcgtc gctgcaggtt
    93541 gccactggat gtgcataccg ggcatggaat acacgatgaa cgacacggtg aaggtcaacc
    93601 tcgccatggt gttccaggtg gcatttcagg atatgatggc caatgccgac cagaccagac
    93661 cgagcatcga tgcgttgtgg gaacagtatc ggaagcactt gaaaatggcc gtcgatgcca
    93721 ccggcagggg aatcatccat cacctcacgc atcagaccaa aagccagccg gaactgattc
    93781 tgaatctgtt ccagcacggt ctcatagaaa aaggcgtcaa tatcacggat ggcggagcga
    93841 actattacaa tatgtgcatc gacggggccg gcctcgccgt agcggcggac agctttgcgg
    93901 cctgccagca gcgtatcgac aaggaaggtc ggctcaccta tgaagaactg acgaaccatc
    93961 ttgatgcgaa tttcgaaggc gagaacggag aatatgtccg gcagatgctg ttccacagcg
    94021 agcggtacgg cggtggcggc gattcgctcg gagacaagtg ggccgtaaag atttccaagc
    94081 tgtggaccgg cttggtaaga gatctggtcg aacagcataa ggacaatccg aagaatgtca
    94141 acttcattcc cggcttcttt tcctggtcga acaccattct gctcgggtcg attctcaagg
    94201 cgactccgaa tggacgcaaa gcgggagaac cgatcaatca cggagcgaac ccgaacggcg
    94261 gatttcgcaa agacggggcc gttaccagca tgtgcaactc gatcgcggcc atacagcccg
    94321 gttacggaaa taccgctccg gttcagctcg agatcgatcc ccagattgcc aacgatccgg
    94381 agggcgtaaa gaagatgtgc gccatgattc tcggaatcat gaactcggga aacacgttgc
    94441 tcaacatcaa catcatagat gccaaaaaga tccttgaagc acataaggat cctttcaagt
    94501 atccggatct tgtggtgcgc gtcaccggat tcaccgcgtt cttttccatg ctgagcgaaa
    94561 agttccgcga attggtgatc gagcgggtga aggcggtgaa tgccgacttg gaatattgat
    94621 atataagttt tgataagaac atgaggagga atgtcagatg agagaagtac cgaagatgac
    94681 cgtggacttc aatgtcaaaa agaacgggag cgttgccctt ttacccggat gtattcccgg
    94741 agcgacactg tatcaccact ctgtgatggc cggttccacc ctttcactcg aaccggggcc
    94801 gttccaccat atcttgatac tcatttcggg tgatgtggaa ttcagtacag acggcaacag
    94861 ctacatattc catgaaagag tctcttttgt tccaaatccg aagctttcgg tcgatatcaa
    94921 agcgctcacc gattgtcact tgatcgaggt ccgctgggag tggcaggaag gcgacgacga
    94981 actggcggcc gaatacaaaa cccaattccc gcacatccag atctaccgca atgcgaaaca
    95041 gtatcgcgac agaaacaaga gcgagaagac catcagccgc tcggtcatcg accagagaat
    95101 catcccgagg ttcgcattcg gatcggtgga aacgtatggg gtcgacgcga tcaaatcgca
    95161 cgaccacccc atgctcgacc agtatttctt cagtttcccg gaaaatgaga tggacgtgct
    95221 catcgatttc gagccgatcc ccatgaaagg gaacgaactg ctgtacatcc cgctcggttc
    95281 catgcacggt gtggacgtca aagagggaca gcactgccat tatctgtgga ttgatttcta
    95341 tccggataac gagcttgctc tcaaacggct cgacgcttcg catatcccga ccggcaaaca
    95401 cgtcgagttc gaccccaaga cgggcaaact caagaaagaa tagccggggg tgttgtggtg
    95461 agctataccg cacccgtgaa acttgcttcc gctcgtgcat ggagaaccta taccggcgga
    95521 agcaaaatag atgaaatcca cggaatattc gacggaaaag actcccaatt tcccgaagaa
    95581 tggatcatgt cgacggttgt cgcgcgcaat gtggggcgtg aagagttcgt ccatgaagga
    95641 ctgagttttc ttgaacattc tcaggaatcc cttcgcgatt tcatagctcg caatccgaaa
    95701 gacgcacttg gaacaaccca tttcgatagc gtcggagaca ctaccggggt cttggtgaag
    95761 atcatcgatg cagctgagcg tctggcggtc caggtccatc cgaacaagca gaaggcgaga
    95821 gaactttttc attcccgatt cgggaaaacc gaatgctggc acatcctcgg aggacgcgat
    95881 atcggcggcc gaccctacgg catttatatg ggattcaagg aaggcatcac gaggaaagag
    95941 tgggaacgct gcttctatga acaggatatt ccggctcttc tcggtcatat gcactatttc
    96001 gatgtgaaac ccggggaaac atatctgatc aagggcggtg ttcctcatgc tatcggacaa
    96061 ggatgtctgc tcattgaaat tcaggaaccc accgactaca cgatacgggt ggaacgcact
    96121 accccgtcgg gtctatccgt tccaccccaa gcgtgccatc agggcatcgg tttcgagcgc
    96181 atgttcgatt gcttcgagta tgatggaatc tctcgagagg aagcatatgg cagatggtgc
    96241 atcctcccga agattctgca acagaccgat gattttacga aaagcctgct tatcgggtac
    96301 gaggatacca cctgtttcag attggaacgc tacgatatga cgggcaagtg tgaaatacgg
    96361 actgacggaa tattctgtgg tctctatatc tattccggtt caggcagcat catatccgaa
    96421 gatcttgtgc agcatatcaa aggcggcgat cagtttttca ttccggcaaa gtgcgactcg
    96481 ttcacgatcg aagccgaaaa agaccatccg atttcaatgt tcagattctt cggaccgaag
    96541 cggtaggtga agtgatggat gatgttgaac gaagagcggc tgaaataccc ggcagtacca
    96601 tcataatggc tgccgaatac gatgtcgtcg tgatcggcgg cggcatcgca ggctgtgcgg
    96661 cggctctcgc gtcgagaaga aaagacaaga aggttctcct gctggagaaa ctcacggtgc
    96721 tcggaggcct ggcgacgacg ggtcacatcg ttatctacct tcccctcgac gacgggtatg
    96781 gacgacaggt gatcgggggc atcagtgagg agcttttgag actgtcgatg aactatgcat
    96841 attacgccgg cgacatctct ctatggaaag aacaaggcag gcgctacgag tgcaagtata
    96901 acggaccagc cttttctttg gctcttgaag aatcattgct tgccgagggc atcgacatcc
    96961 tgtacgacac gctgttcgtc ggttcagaaa ttgaagacgg tgtctgcaag gcggtcatca
    97021 ccgaaaacaa gtcgggtcgc agcagatacc gctgcaaggc ggtcatcgac gcatcgggag
    97081 atgcggaagt gttcgctagg gcgaacgaac cgtgcatggc cgccaagaac agcttggcga
    97141 tctggagcta ctgtacgcaa ggtgcggacg cccaatatca gaagcgaggg ggtcccgacg
    97201 gacccggcct gagtctggtc agtttcggaa aaatcgatac gcaggctttc aagcatacgg
    97261 tgacggtccc ctattacgga gataccgccg aagggatcaa tcgtttcatc atcgacggtc
    97321 atgcgcgatt gctgaaccat atgaaagcgc atgacgactt cgttccggca tcgcttcctg
    97381 ggatggccca gatacgcatg gcgcgcagga tcgtaggaag gtatgagctt tccgacaagg
    97441 atgccggaag acattttgaa gacaatatcg gcggcacggg ggattggaga aaaccgggac
    97501 ccgtgtatga gattccctac agaacgctca tgacgaggaa cctgaagaac gcacttgccg
    97561 caggacgctg catatcctca agcggcgagg catgggagct gacccgctgc attcctcagg
    97621 cggcattgac gggacaggct gcgggaaccg cggcggcgat cgccatcgaa cgatcgctct
    97681 ccgtccagga tattgatatc cgtcagttga gagaaacgtt gaaacgtgac gggatgattc
    97741 tcgatgtcaa ccaatgagaa gttggagcgc cgcatggaga atacagtcag aatagggatc
    97801 gtcggatgcg gaatggtggc aagataccat gcgaaagcga tcgccgaaac gccgaacgcc
    97861 atgctcatcg gcgcttgtag caaaagcctg tcgagcacac ggacattctg ttctgaattc
    97921 tcgcaagtcc gtgcatacaa tagttatgac gaaatgctcg cggaacatga tatcaacacg
    97981 gtcgctgtat gctccccgac cgggaatcat tacgagcaag cgaaaagagc gcttgaagcc
    98041 cataagcacg tggtcgtcga aaaaccgatc tgcatcacgc ttcgccaagc tgacgatctg
    98101 atccgttttg ctgatgaaga atccttgagc ctatgcgtca tctcgcagac gaggttttcc
    98161 gattccgcga cgatcataaa acaggcgatc gacaatggtg aactgggaag gccgatatcg
    98221 gcttcgctga tgatgcgcta tatgcgtacc caagcttatt acgatcaggc ggactggaga
    98281 ggcacgtatg cgtacgacgg cggcggagtg ctcatgaatc aaggcatcca cggaatcgat
    98341 ctcctttgtt atttcatggg aaaaccccaa tcggtaacgg ggtatgccaa gaccatgctc
    98401 agatcgatcg aagccgaaga taccgctgtt gccgctattg aatttgaaaa ccaatccgtt
    98461 gcggtgatcg atgcgaccgt ctgttctcag cctccgtttc ccaaacgatt catcctttgc
    98521 ggggaaaagg gaacgatcat cctcgaggat gacgcgatca ccttatggaa tctgccgact
    98581 ccctgcccga ttgagataaa agcgagtgct ggcggttcgt catccgccga tccgaaaggc
    98641 atcactcata tataccatgc cagagaatac cggaacatcg tcaggcatct gctgaacgga
    98701 gaagctttac tcatcgacgg tcgtcagggc aggattccgc tgtcggtgat tttgggagta
    98761 tacgagtcat cgaaaaccgg taggaaggta ctgttatgaa atatgcattg atcggttgtg
    98821 gaagaatcag caccaaccac atacgggcgg ctgtccataa tagtcttgaa atcgcgggag
    98881 tctgcgacat cgtgggcgag cgcatggatg cggtattggc aaagaacagc ctaaataccg
    98941 attccaccat cgcccgatat catgattacg aacggatgct gaaaaatctg aagccggatc
    99001 tcgtcagcat cgcgaccgaa agcggaaagc atgccgaaat cgccatttcc tgcatcaatc
    99061 acgggtgcaa cctcatcatc gaaaagccga tcgcgatgag tcttttcgat gccgatagaa
    99121 tcatcgaagc tgcaaagaaa atgcgggtga aagtcgccgt atgccatcag aacaggttca
    99181 acattgcggt tcagaagacg agaaaagcgc ttgaagcggg acgtttcggg aaactcagcc
    99241 atggctcgat tcacgtccgc tggaatcgga atcgggaata ttatacccag gctccatgga
    99301 gggggacgtg ggaggaagac ggtgggtgcc tgatgaacca atgcatccac ggcattgatc
    99361 tgctcctgtg gatgatgggt gatgagatcg agtccgtcta tggagcgacg cggcggaggt
    99421 ttcacgatta tctggaaacc gaggacatcg gcatggcgct cgtacagttc aagaacggta
    99481 cactcggaac catcgaaggt actacgaacg tctatccgaa gaatcttgag gcgacgctct
    99541 gtctgttcgg agaaaacgga acggtgaaga tcggcggaac cagcaccaat accatcgaag
    99601 tctggaattt tgtcgatgct tcagcttccg aaaacgagac actgattgaa aaaaccgcag
    99661 atatctatgg caacggccac ttgcatctct ttgccgatgt catcgatgcc atcgtcaatg
    99721 acagagatcc gtatgtcgac gcgtatgccg gcaaaaaggc ccttgaagtg atcctggcca
    99781 tctataagag ccagaagaca ggactaccgg ttacctttcc ccttgatgat tttgcaagca
    99841 ccgacatgat tggaacgttc tgaatatgaa gccagtatgc ttggagttga tgagtgaagg
    99901 tatttatata gtgatggcaa ggtggtacga tatcggtata tcgagttcat cgtgaccaat
    99961 tagttcaggc tttaaaggtt catcaaatcg aaacccctca catagctgaa gaagtacttg
   100021 cgaaaaagtc ggatttctac cgattccaag ttactccgtt cggtgagcat gtaagatttg
   100081 aggcagtgtc gacttgggcg tatgggtgga tatcagaacg ccaccaatag tcgtgccagc
   100141 gcacaatctc ttcgctccac gccagatata actcatagga aggatccaac tgagacggat
   100201 ggatgaattc ttgcggctaa tcacccttgc ttgaaacaag caataccatt ttttccagga
   100261 tgacatcctc tgcgatgacg ttcttgtaac gcgactggtg gaatacgaag ccgacatcca
   100321 cttcatggtt attgatcatg ttgtaaatcc cggggctttg atgggttctg atgcgcaaat
   100381 cgacatgagg gctagagtgg agcaacttct cgtacaaggg gcttaacagg tagatattca
   100441 agctggacgt caccgacagc ggcatccccg tcagcgccat cgttaccggg gcgaacctgc
   100501 atgacagccg ggcggccatc cccttggagc ggatgaccgg acagcggatc agacacctgt
   100561 attcgctgat ggacagcgcc tacgatgcga agcctatcag ggacttcatc accgacaacg
   100621 gaaggatacc cattgaggta tcacgtgcgt gttcgatcca ctcatatcat tttcttgtgg
   100681 attcccgtgt tggtgatata taatgagtta acgtgcacgc aaaaaagggg catgtcgatg
   100741 actcataaca aaacgcaaac cggaaacctg ttccttgtca tccttatcat catggtcgca
   100801 tcctgtcttg tcggatgcgg cgctcagaag aagcctgctc aatttgaaga acatgacggg
   100861 atcatcgtag ggttctccca gatcggtgct gaaagcgcct ggagaacatg caacacccgc
   100921 tccattcagg aggctgcggc cgaacggggg gtgcagctgg tgtacgacaa cgctgaacag
   100981 aaacaggaaa accagatcaa agccctgcgt tcgttcatcg cctatcaagt cgatgtgatc
   101041 gtcttcgtgc cgatcgtcac ggatggctgg gacaacgtgc tccaggaagc caaggatgcc
   101101 ggaatacccg tgttggtcac cgaccggaag atccgtgtcg aggatccctc gctctattcg
   101161 ggattcatcg gcaccgacag catcacggaa ggccggaacg gcggactgtt cctattgaac
   101221 aaattcaaag ccgaacgcga ccggttcgag gaaaccggag aacacatcaa catcgtggag
   101281 atgttcggga cggaaggctc ctccgtcgcc atcggacgag ccgaagggtt ccgacagatt
   101341 ctggatgacc atccggagtt ccggatcatc tacagcaagt cgggtgattt cctccgttca
   101401 agggggtatg agctggccgt cgagtttctc gagcaatatg acgacatcga cgtcattttc
   101461 tcccataacg acgggatgac cctgggagcg atcgaggcga tggaagaaag aggaatccga
   101521 cccggcatcg acatcgtcat cgtaacgatc gatgcccagc aggcggcgat cgatgcgctc
   101581 cgtgaaggca aggtgaactg cgtcatcgag tgtaacccca agaccggtcc ggagatcatc
   101641 gaactggcag agaagctggc ggccggcgaa agcattccga aactgcaata cgtgcatgaa
   101701 gaagtgttct atgaaaccga cgacctctcc ctcgtcgcgc cccgcggata ttagcatgaa
   101761 gaatcggatg acccttattg acaagatctt ctcggcgttc aagatccgaa gcatcaagga
   101821 gagtataaag atctcctatg tgatcatcat tctcctgatg atcactcccc cggtcatcac
   101881 gatcggctcc ttcgtcatcc agatggtcag atacgacctc atcatcacca acgtcagcaa
   101941 ggcgaaccgg ctcaaccaga cggtgaagat ggatgtctcc aacgaaatat gggacatcgt
   102001 ggcgggaaac aagaagttca atgaaggcga tcaatacaag atcatcgacg gaatcaacga
   102061 gagtctggaa gacatcatga gcaccaccga agagcgggaa aaccgacaga tgctcgaggt
   102121 ggccggtcgg gcggtcgata cgctgaagcg gaacgtggat cgtttgggaa gccagatggc
   102181 gagcagttct ccggtgagcg agaatgagga gatcctcgag gagatccggg gagtgtccgc
   102241 cctgatctcg gacatcctcc aggatttcat cgtgcgggtg atcgagtcgg cgacgattac
   102301 gaatgagcat cacaagcgga tcaccctcgt ccttacggtc atccaggttt ttaccgtctt
   102361 tctcgttgcg atcttcgccg tcttcaccca gcgctccgta accgccagca tcaacgatcc
   102421 catcaaacga cttgaacacc tttcaaaacg aatcgccgaa ggtgacttca ccgccagagt
   102481 gcagcttcct caggtgagcg agcttgacgg gctgaccgac aacctgaaca tcatggcggt
   102541 caagatccag gccctgatcg aagaaaacat ccgcaagcag cagctcatcc agaaatcgga
   102601 gatgaaggcc cttcaggccc agatcacgcc acactttctc tacaacacgt tcgacacgat
   102661 cgtctggctt tccgaagaaa agaagaatga ccaggtcatt gacataacac gctctttttc
   102721 aaactttttc agaatctctc tcaacaaagg caaggacttt ctgacggtca gggaggagtt
   102781 tgaacatgtc aaaagctatc tgagcatcca gaagatcaga taccgggaca tcctggatta
   102841 tgaaatcgag tacgcgcccg agatggcgga gtgtcaaatc ttgaaactgg tgctccagcc
   102901 ccttgtcgag aacgccctct accacggaat caagaacaag cgggcccgcg gattcctgtc
   102961 ggtgaagggt tggcgtgaaa acggatacct ttgcttctcc gtggaagacg atggaatcgg
   103021 aatgaccgag gaaaagcttg aaaatatcat gaaacagatc agcagttcca ccaacccgga
   103081 ggatctgagc agcatttatg gactgtataa cgtcaacaag cgacttgagc tctattacga
   103141 tgcaaccacg aagctggaca taacgagccg gtacaaacag ggcaccaccg tgtttttcaa
   103201 ggtgccggag gctggattca atgtataagg tgttcattgc ggaagacgag atagtggtgc
   103261 gtgagggact ccgaaacacg atccagtcgg ggacaggccc cttcgtcctc gcaggtgaag
   103321 cctctgacgg cgagatggcc ctgtcgatca tgaaggatgt gaagccggac atcctgatca
   103381 ccgacatcag gatgccgttc gttgacggac tgagcctttg ccgaatcatc aagaagattc
   103441 ttccctggat aaagattatc atcatctcgg gacatgacga gtttcaatac gctcaggagg
   103501 ctatctcgat cggtgtcgat gagtaccttc tcaaacccat ttccgcttcg gacatgctcg
   103561 aaacgctcaa caagctggtg gatcgaatcg aacaggaaaa aaagcatctc tcaagcatcg
   103621 aacatctgaa actgcaggcc cagtccagtt cggacttgat cagggagcgc tggttaagtg
   103681 atctggtgac cggaatcgtc aaaaccgagg atgcacttga gagcgcaggc gatatgggaa
   103741 tcgatctcat cgctcacagc tacctcgtag cgatcatcaa gctttccacc tccttggaaa
   103801 actattccga cctgatcacc gcgaagcttc atatcaacag tctcatcgac aatcaggaag
   103861 aggtcttatg cttttcacaa agcagggata cgatcatcct gctgctgatg cagctcgtgt
   103921 ccgaatcgct cgaagagacc gcctatacat taggccaagc aatcaaatat gaggttgaac
   103981 gaaacaccag gtgcatggtg gcatttggaa taggctcgtt ggtcgagcgg atcggaagcc
   104041 tgtcccaatc ctacgccgat gcggaaaaag cgatacactt ttccgtcaag accggtcaga
   104101 aactcatcat cggaacccat gatctgagtt ctttttccga aatcgatttt ttgaagctgg
   104161 atggaagctc catatcggag cggctcaaat atgtgaagaa atccggtgtt gatgagatta
   104221 tcgctcaata cgtcaccatg ataggcgatc agcctcttca gaccaccctc ataggctatt
   104281 accttctcta cgatctcatg gtggcgatat cgaagatcat cgatgaactg gggggtgatg
   104341 ttcaggaggt cattccgtcg ctgcctcaaa agacccagct ttcggagatg gcggcatcga
   104401 aagaaacgtt ctgcgacggg gtgaaactga ttctcgatac gttcatcgag ttccgggatt
   104461 ccagatctgc ggggaaatac tatgaaatga tccagaaagc gaagcagcat ataaaccttc
   104521 atttcgccga tcaggacatt tccctgcatt cggtggcatc aatcgtccat gtgagcccga
   104581 atcatttcag cacgattttt tctcaggaaa ccggtgaaac cttcatcgaa tatcttaccg
   104641 gggtccggat caataaatca aaagagctgt tgttgacgac agctctcaga agtgcggaca
   104701 tcgcctatga agtgggattt ggagatcccc actatttcag cttcatcttc aagaaacaca
   104761 ccggcatctc cccccgggaa ttcagaaccg gaaacaaagc ccaaacctga aaacagcggc
   104821 tatgcttgcc ccttgcccga aagccgcagc cacggcggga gcgcgacagt gaaccttttc
   104881 cgatcgcgaa gcgacatgat gatgctttgc aggatgatga aaagatacaa cagcaggccg
   104941 cttacgatcg cctggaagtt ggattggact cccgctgcac ggatcagttc gttgatcacc
   105001 agaagcgtca acgtgcctat cggcgccccc agcaggtttc ctaccccgcc gttgagtagc
   105061 gtaccaccca tgatggctga agcgatcgct ttcatttcgg ctccggccgc attgccgata
   105121 ttaccggccc ctgtcgtcat gatgtacacg aacccggcga tgccggccgt caatccgctg
   105181 atcacgtatg tgatgaattt cgtccgcttg acgttgatgc cgagcatcat ggcgctgtgg
   105241 ctgtttccac cgacggcata gacgttgcgc ccgaagcggg accacttcat cagggatgcg
   105301 aagatgacaa gaagaaccgc gacgatgatc gtaccgggtt ttatctggca cgggatgaag
   105361 acccccagtc tgtttctcgt gccgagccat gtgatcacga tgttgaaatc ccgaaggttc
   105421 acgaatgcgg gcatcgtcac gttgagcgga tccttatgga gggtcgtgag gagtccctgc
   105481 gccaggaaca agcctgacag cgtgatgatg aacggctgga tctcaagata tgcaatgaga
   105541 tacccgtgga ggattccgaa cacgatgccg atgcccatcg aaagaaaaat cgctcctgcg
   105601 atgctccccg atcttttctc cagatagact gcgcaggcca tcgtcaccaa tccgcagacc
   105661 gagccgatgg agatgtcgat gccgccgccg atcatgacga tgcacagacc gaaggtcatg
   105721 atgatgagcg gagcgttgag gttgaacaag tcgaaaaacg tctggaactg caggaagctg
   105781 cccggatagc tgatgatcgc gaacaggtac atcagcacga atatgaccga agcgatgagg
   105841 aacagaaggt tcgaattcga cagtctggct ttttgtttga tcgctttttc ggatgccatt
   105901 ctattcctcc ttccttgttc gattcctgtc cagaaccttc ttgatgaaat ccctgaccac
   105961 gggagaagcg acgaccatga gaatgacgat gaagatcgcc ttgaagacct tgatcatcgc
   106021 aggttcgatc tccagcctga ggagcgtcct gttcagcatc tcgatcgtgt acgctccgat
   106081 gatcgagccg gtgatgctga acgttccacc tcccagtgag ttcccgccga tcgccacggc
   106141 gaggatcgca tccatcatga tgtttttaag caggttgacg ctgtcgtgcc gccccgcctt
   106201 gttcactgcg atgaaccctg caaccgctga acagaatccc atgatcagga aggttagaaa
   106261 gatgactttc ttcgggttga tgccgttcag ccgggcggca ttcgggttga ttccgacggt
   106321 ttccacatag agcctgagcg tcgtggtctt cagcagcacc gcaaagaggg cgatgaagac
   106381 gacagtgagg atcaccggag tctggatcgg tattcccggg atcacggtgc ctatcctgtt
   106441 cgatatgtcg ttggccagaa tgggagacag tttgccgtcg atcatgaagg ctatcgacct
   106501 tccgccggta aacaggatga gggatgcgac catcggctga accttgaaga tcgaaacgag
   106561 gatgccgttg aaagcgccaa gccccaaacc cacgacgcag ctgagcagga agccgacgat
   106621 gatggtaacc ggagtggcct cgccggatcg aaggacgaac cagacgaata cggcggaaga
   106681 gatcgtggcg ctctccccta tgctgatgtc ctgcccccgg gaagccgcgg tcaccagcgt
   106741 catgccgatc gacaggatga ccagttcgga agccccgaac aggatattcg gaatattgcc
   106801 gtagagcgct ccgtttatca tcgtgatggt gaaataatca gcacctttca ccagattgat
   106861 gatgatgagc agcaccatga cggagatggg aagaaccaag tgggaaacgt ttttcatcaa
   106921 ttttcgcatc agctctttcc cccctgcgcg atcatgtgca tcacatcgtt ctccgtcagg
   106981 tccgttcccc tgagttctcc tacgatctca cgatccttca tgacgatcaa tcgggagcag
   107041 gttcggagca tctcgtcgat ttcggaagag atgaaggtca ggctcattcc gccggaagca
   107101 agcctaatca caagcttctg gatctcgacc ttggttccga catcgattcc ccgggtcggt
   107161 tcatcgagaa tcaggtattc gaggttcgta agcagccagc gggcgagaat gaccttctgc
   107221 tggttgcctc cggaaaggga tcgtatcggg gtgttggagg tcggagtctt gatgttcagc
   107281 tttttgatgt actcttcggc gaacttttcc gcctctttca acgagaaggg cttgaacagg
   107341 cctttcagaa cctgcagggt gaggatgatg ttgtcgcgaa ccgacaagtc ctcgatgatt
   107401 ccgtccccct tgcgatcctc cggcagatag cctatcccat gttttatggc gtgcaggggc
   107461 gttttgatct tcaccctctt gccatggatc ctgacttcac cgccggtcac cttgtcggcg
   107521 gcgaaaatgg cgcggacgct ttcactgcgt cccgagccga gcaatccggc aaacccgttc
   107581 acctcgcctt tgtggatggt gaagtcgaac ggtctcaccc ctgcggcact cgaaagcccg
   107641 acggcttcga agacggggat cccggcatcg gatgcgaccg gctcatgttt tcgtatcgtt
   107701 gaaatatcct caagctcact gcccagcatt ttcgagatga gttcgatctg gggaagcgag
   107761 gatgtcttgt attcacctat gagttttcca ttgcgaagaa ccgtgattct gtcgctgatt
   107821 tcgtatacct gctcaaggaa gtgagtgatg aagataatcc ccacgccttg ggctttcagc
   107881 gctcgcatga gttcaaagag cttttgtacc tcatcttcat caagagagga ggtcggctcg
   107941 tccagaatga ggattctgca gtccatgtcc accgcacggg cgatcgcgat catctgctgt
   108001 accgcgatcg aacaggttga aagctcctgg atcgggcttg ccggaatgcc gagcgagtcc
   108061 agcagccgcg ccgctttttc gttcatctct ttccaattga caatgggagc gtggcttcgg
   108121 ccgatgaaca tattctccgc caccgtcaga ttgggacaca gcatgatctc ctgatagacc
   108181 gtaccgattc cgttgttttg tgcttcctgc ggcgatctga agtggatggg cttaccatgc
   108241 aacgtaatca agccggcgtc tttttcgtag actccggtga tgactttgat cagtgtggac
   108301 ttgcctgccc cgttttcacc catgagagcg tgaatctcgc cctcccgcag cttgaaatcc
   108361 acgccatcaa gagctttgac cccgggaaat gatttgctga tgtccttcat ttcaagtatg
   108421 gtttccgaca acaatcaaag atctccttac cctatgacta tacaagatga ttgcggtgcg
   108481 cggaatcgta tccgcacacc gcactgcggc aaagacctac cttgtgatga cgcccttgcc
   108541 cgggtcgtcg ttgatgcccc atttctcgat gtccgcatcg gtaatggtat cggtggtgaa
   108601 gagcagctct tcctgataga cgataccgct tggaatgttt ccgctcttga tccactcgga
   108661 aatcgtggcc gcttggcggg ggttgcactg catatcggca tcccagtagc cggcctgaac
   108721 gttccgaagg gcgaatcggt tgaagtcgaa tccgatgacc ttgaccttgc cgttctttcc
   108781 gtggctgatt ccggccgctt cgagcgcctg aaccgcacct tgcgccatgc cgtcgttctg
   108841 ggcatagatg acgttgaaat ccttgcctgc tgcgatcgcg gcttcaacga cttttcgggc
   108901 ttcctcaaga ctccagctgt ctccgccggt tccatcggcg acgatgttga gcttccctgc
   108961 ttttgcggca tcgagcaccg ccttgcttcg gccgatctcg gctgcggaac ccatctgccc
   109021 ccggataagg atcagattga tcttactgag acccagactc aacacccatt ccaccgcctt
   109081 ctcgccttca tacgccatgt cggagagaag cgccgcttga taattagatg gatccgtatc
   109141 gatcgcccgg tcgaagagga tgactttcac acccgcttcc tttgcggact tcagggtgtc
   109201 atcccatccg gaagcgttcg ccgcggagat caggagatag tccacaccat cacgaatata
   109261 gcctttcgcg gcagcgatct gctcgctgtt gtccatcgtg ttggtctgct tcgaatcata
   109321 tccgttggct ttcgagaaga cattgttcat gtcttcgacg ttggcctgtc gatatccgga
   109381 ttcttccggc ggcaggttca cgatgcccac tttcgtcagt ccggtctctt tctgaccggc
   109441 ggcgaacagc acgcccgttc ccagcaaaac cagaaccatc aaaacacata gagttttttt
   109501 catgttctcc tccttttcaa ggaatcagaa aaattacgaa taccagcggc aacgccgcat
   109561 caccaataaa atggtaagac ccgatttttc agaagtacat gagggattct attgagtttg
   109621 ttttgatatt ttacgtattc ataacaaatt ctgaaaaaag attggttatt ttatgaaaaa
   109681 gattcgtttt cttccgatat atgatcgaag aaaactaata agttattgca aagcatatag
   109741 ttctcgggag agtcatagga tgggaatatg gagctggaac tgtacatagt tgcaatccag
   109801 ctccgaaaag gttgatatct ttgcagaaga gggatctctt tgagtcagcc cgccttcatc
   109861 tcatgtacaa tcccccgttg acatccaacg ctatccctgt aatatagctg gacagatcgc
   109921 ttgccaagaa aagcacggca tcggcaatat cctcagggac accaaaacga ccgagaggaa
   109981 tagtgagttt gggatcgtct ttcggatcgg tcaaccagct caagcctttg gccatttccg
   110041 tgagagtcct tccggggcat actgcattgc ttgtaatgtt atattggccg ccattgagtg
   110101 caaagctctt ggcaagattg atgacacctg cttttgatgc ggaataactg gctgaagagc
   110161 ctcgtcctcc ccgttccccg gccatggagg aaatgtggac caaccgccca taacgctgct
   110221 ctttcatgat tttgaaaacc gcttgtgaac agaaaaaaac gctcttcaaa ttgatgtcct
   110281 gcattcgatc ccactgttgc tgggtaatac tctcaatgga agccgattca gtgattcctg
   110341 cgttgttcac ccaaatgtca atacttccaa ataatgcatg aatccgctca acctgcttgg
   110401 gaatgttgtc aaaatcggct acgttgaaca ttaacgactt cgcttcatag cctttcgcca
   110461 ccaagtcttt ctcaagtgtt tcaattcctt ccgtattgat atcggttaaa acaaccttcg
   110521 caccgtattc cgccaaacga cgagcgatag ccgcaccgat tcctcttccc gaacccgtta
   110581 ccacagcaac tctaccagcc agcaactctt tcatgtttat ttaccttcac ctctagtttt
   110641 ccagaacaca tcgcagtgcc ctattccatc cttcaataca tcgatttcga ccttcactgg
   110701 acatagtcgg ttgatattca tgatatatgt ccaaagcttc acgtgttcgg tcatcatata
   110761 atcctaaagc ttgaccgact atcatcgcca ctccgaaaca ggtcatctcc tcatgatcgg
   110821 gcacatggac cggacagttc aggatatcgc tttgaaactg catgagatac ctgtttttca
   110881 ctggaccacc cgcagctttc atgaatggga tggcaacctg ggcatccagc gacattgctg
   110941 aaaccacatc ggagacttga tacgcgatac tctccaggca ggccttcacc agctctgctt
   111001 ttcccgtcaa acgggacatt ccgtaaaaca tcgcattgga ctcattcttc cagtgtggag
   111061 ccccgatccc cgagaaggcg ggaacaaggt atgtccgatc ctccttattt gcctgctctg
   111121 ccagcgagca agtctcctga ggagacgaga ccaatcccaa atccttgcat atccatgtga
   111181 tgacggcacc cgaataattg atcacaccat caaaaatata tcggacatgc ccatcgatct
   111241 tccatgcaat tgaagtaagt actccatgtt tggatatgat gggagtatct cccaagttca
   111301 tcatgacaca gctacctgta ccatacccgg tcatgcacat gcccttctca taacatccct
   111361 gtccaaacag actggcatga gaatccccta tggcacacct gatcggaatg ggcttctcaa
   111421 acaacccatc catatcggtg aaaccaaaag atgcatccga atcacaaatt tcaggtagca
   111481 tcgatttcgg gattcccaac agggaacaga ccccgtcgtc ccatgtgccg gtgcggatat
   111541 tcatcaacat catgcgagat gcgtttgaat agtcggtttt atgggaagaa cctttggtga
   111601 ggttgtatat gacccaacta tccatcgtgc cgcaacacaa acggccttgt tcggcctttt
   111661 gccggactcc ggggacgttc tcgagcaccc atgcgatttt cggaccggaa aagaagggag
   111721 agagacgcaa cccggttgtc gactggatgt aatcggcaac atcagccaat cggtcacaaa
   111781 tatccttggc cctgttgcac tgccacacaa tcgaattata aaccggccgg cctgtgacgc
   111841 gatcccagat gattaccgat tcccgttgat tggctaatcc cattgcgact attcggcttc
   111901 tgtccacccc gctcttctca agaatggccc tcacagccaa atggacattt ttggcaattt
   111961 ccatcccatc atgctcgatg tacccacgcc ggtcgatgat ttggcgatgt gcgacaacct
   112021 cactggcgat acacttgccc tgttcatcaa aaagcatagc cttggtacct tgcgtgcttt
   112081 gatctattga aacaatatat cgcttttcca taccaattct ccgtatcttt caattacaaa
   112141 gtgaatggat gcttgccctc aattaccgat gtatcgtact tggcatgatc tttctgaaca
   112201 aacccggcga ttcgcacata cgaatttttc agatgctcgt gcagatgaga gtcttctccc
   112261 cacttgatca cctcgaattc tttgttggaa gaaaaatcta tggtggtgat cgaagtgttt
   112321 tccaacctga ccattttcca aacttcacgg attggaatcc ccaataattt tgacatgatc
   112381 aactgaatcg tcagcatatg ggtaacgatt gcaacgcttt tgccggcttc tttttcaagg
   112441 atttctttga gggcttggac ggatctgtct tggacctgtt tgaaactctc cccgtcaggc
   112501 atgtgcagtt catcgggacg gtgctcccac aaatcgatca tgccgggcca gcgttcctcg
   112561 atttcggtac ggaccaagcc ttcccactga ccgcaatgta tctcactcaa cttgggttca
   112621 ggaataacgg ggatatctct gccagccaat acctgcttag ccgtctgcat ggttcgcttg
   112681 tatgggctca catagatctt gtccaagtgg aacttgctca tcggggacca caagcaggag
   112741 gcttgttcaa gcccacgttc attcaaggga ttgtccgtcg agccttggaa tcgatgttga
   112801 acgttgtagt cggtttcgcc atgcctgata aagtatattc ttgttttcac gtagctctgt
   112861 tccctcacac tttgtgcaac gcgctcttga acgcatccaa cgcagcttct ttcaccattt
   112921 ccgacaacga aggatgagcg attaccagat gcttccaatc attgaacgtg aacttcttgg
   112981 cgacagctgc ggtagccaat gaaatcaatt caggcgcatg ctcacctaca atctgcacac
   113041 cgacagtgat cctgtgttgc ttgtccatga tgacgtacac actaccctta gcctcttcag
   113101 ccaacgccat accgttggct tcatacgaaa aggtgcctac gaccgtttcc acccctgcct
   113161 gctccgtagt cttgcccact gcggcaaaag cgggaattgt gtaaacacaa cgaggcatta
   113221 cggaatcatc gtacggcgcc ttcgtgtctt ttgcgaggat agtatccaca accgactcgg
   113281 cttcggcata cgctgcatga gccaattgat accccccgat cacgtcaccg atggcatata
   113341 ccccatcaac acttgtctgc aaataccggt caaccttgac ttgctttgaa tcagtaagga
   113401 ctatctttga atcaggagtt atgcagtcgg tattgggctt cctgccgaca gcaatcaaaa
   113461 ccagctccga ttctatcgag ccttccttgt cacccactgt gaaaattgtc cggcataccc
   113521 cgtctctctt ttcaatcctg ttcacccgcg cggaaagatg gaatgttaac ccattctttt
   113581 ccaaggtttt tttcagttga gtccccgctt ggggttgttc tttcggcaac agagaatcca
   113641 tcatctcgat gacggtaact ttgctgccga acgaattgaa agcactggaa agctccagcc
   113701 cgattacccc gcctccaatg accgtcaagc gtttcgggat acttgggata gccaataaat
   113761 ccgtagatgt gatggcatgt tcgactccgg gaatactgat catcgccgga cgggatccgg
   113821 tggcaatcac gatatggttg gctgtgtatc gtttcccgtt gcattcaacc actttgtcgg
   113881 ctttcagaat cgcttctccc ttgaacaccg caacgttgtg atgctttaac agaccattga
   113941 caccgccaac cagcttttcc accactttgg tcttttgtcg ctggatttct ttgaagttga
   114001 acataccggc atccttatag attccggctt cagtcaacat cctgattttc tccaagccac
   114061 ttgccttgtc caacaggtat ttggtaggta tgcaacccac attgagacat gttccaccaa
   114121 aggactcttt ttcaaatatc gctacacgca tcgaggcttt tgccgccttg atagcacatg
   114181 catatccacc gggaccacca cctatcacta ataaatcata cgtatccatc acatccatcc
   114241 tattacataa acatcatcag cggatgttca agaagcgttc gcaacgcatc aagcaatcga
   114301 gcggcatatg ctccatcaat cattctgtga tcgaatgacc cggtgattgt catcatctca
   114361 atcggcttga agttactacc gtcccagacg ggcttcatct caatgcttcc gatggcaaaa
   114421 attgcgcttt cgggtggatt cacaattgct gtgaagtgat cgacgccata cattcccaaa
   114481 ttggaaattg taacgctgcc actccccatc tcatccgcaa gcaaaatgcc tttccgtgct
   114541 ttctcgatta attccctgct ggttttagcg atctcttgta ttgacagcat gtcaacgttt
   114601 ctgattacgg gtacaatcaa tccatcttca atcgcaaccg ccagtcctac gtttgtatgc
   114661 tcatataccc gtatttcgtc atgctcataa cgagcattta ccattgggaa atccttcgca
   114721 acaaccgaaa cagctttcgt gatgatgtcg ttatatgaga ctttgatttg acgcaagact
   114781 tccagttgct ttttcaaatc gagtacctga cgtgcatcaa cagaaatggt cacctggaag
   114841 gaaggaattg tggagacact ttccatcatc cttcgcgcga ttgcctttct catccggccc
   114901 atcggcatga ctgtgtatgc ggctgcatcg tgcggccggt cttttacagc tgccagcaca
   114961 tcctgtcgcg ttatatacac gccattggcc ggcttgatgg ttccaagttc cactcccaat
   115021 tctttagcca accgtttgct gttaggcatt gcaggaaagc attctgcttc agcaggcagt
   115081 gccggcttct ccaacggtcg cttcatgatc ggaatgaatt catcgtcctg tccctcttgg
   115141 gtgacaatcc gatcttgtgt ggtctgggtt gtctcgcttt cagcgcttct ggaataggaa
   115201 gccacatcct tcgcatcacc gatgacgcac actacatcgc ctccctcaac cgtctctcct
   115261 tcttgggcga gaatgtcgat gacgatgcct gccgtaaagc tttcgacagg taagactgcc
   115321 ttatcggttt cagcttccaa caaaacatcg ccgcgctgaa tcttgtcgcc taatttgact
   115381 ttccactcga cgatcaccgc cgcatcggtt gtctgcccgg cgttgggcat ccgaatttct
   115441 ttaatcattt cacaccttca tcagttttct tgcttcctcg acaatcctct ctttcgaggg
   115501 aatcacataa ttctccagga caggggagaa aggaatgggc gtatcatagg cggctacccg
   115561 aacgatcggt gcatcaagca aaaagatttg gttttcagct acccaggagc caagttgggc
   115621 accccagccg cagttatagt tatcttcatg gacgatcatc agccttccgg tctttttcag
   115681 agaattcacc actgtctcgt aatcaaacgg aaccagagtc cttggatcga tcacttcaca
   115741 gctgatacct tcctgttcaa gcagttcggc cgcttcaagg gttttataac tcatcagaag
   115801 gtttgcaacg attgtgatgt ctttaccttc ccggcgtacg gcagccttgc caaactcaat
   115861 tacgtaatct tcgtcgggga cttccccaat ggttgagatt gcatctttct ccttacgctt
   115921 gccgccatag agaagcttat gctcaagtac aagcaccggg ttgtcgtccc tgattgcctg
   115981 tttcatcaac ccttttgcgt cataagccgt ggagggacag attaccttca gaccgggtac
   116041 gtgtgtgaag agactctccg gactctgagc gtgctgggct ccgcgattgc tcgctccgca
   116101 gggagcacgc atcaccatcg gcacatgaac ctttccacct gacatatagc acatctttgc
   116161 agcctgattc accagctggt ccatcatgca aaagaggaaa tcactatatt gcaggtcggc
   116221 gaccgggcgc atacccatca tggctgaacc aacactgacg cctgccaaca taatttcaga
   116281 gatcggggta ttcaatactc gttcataccc gaattcctca cctaacccct tggtaacggt
   116341 gaatgctcct cccataccac ccgggatgtc cacatcctgg ccaaggcaga atacgagtgg
   116401 atcgcgcctc atttcctcag caatcgcact tttcaaagca tcagcaatag ataacaaggc
   116461 catttttatt taccctccca atacacatcc gtaagcgcgc tttcaatctg agggaacgga
   116521 gcattttgcg cgtactcgac agcctcatcg atttccaatt caatcgcttt ctcaaccgtc
   116581 ttcaactctt gttccactac caatttttta gaaatcaaat aattgcggaa attcttgacc
   116641 gggtctcgat cgaaccagtg cttctcttca tccttcgggc ggtattggca ggcgtcgttc
   116701 ctcgagtgtc cgccatggcg gtacgtcttc aactcgatga tggtcggacc ttctcccgct
   116761 cgtgcccggt tgattgctct tgttgccgcc tcattgactt tcagcacgtc gttcccgtcg
   116821 acaatttcag ccgggatggc gtacgaagct gccctgtcgg cggccggatt gggcatgttg
   116881 caggtcaagg ctatgctcgt cgtggcgcta tagagattgt tctcgcaaac ataaatcacg
   116941 ggaagcttcc aaatggcggc tgcattcatt gactcgtgga atgatccctc attcgttgca
   117001 ccatcgccga agaaacacac aatgacattg tccttcttct gcatcttgaa cgccagcgcc
   117061 actcccgctg caataggaag gttccctccc acaatcgcgt tggcgacgag ggctcccact
   117121 tccatatcgc cggtatgcat agctccacct ttgccgcggc aacacccctc ctccttgccg
   117181 aacatctcgc acatcatgga ttttagtgag actcctttgg cgaggtcgtg accggccggc
   117241 cgatgggtgg aggccatgca atcatccttc ctcaagtcgt acaacatccc aaccgcacat
   117301 gcttcctggc ctgtactttg atggatcgtt cccggcatga ttccttccaa aaacaaatag
   117361 taaatgcgct cctcatactt acggatcaga tacatctgcc gatacatgcc gagcattctt
   117421 tccttgtcag gtaacttgct catttttttt ccgcctttct ttttgtaatt acatttattc
   117481 ttatcatata actttttaaa accgaagaga aatgtaacta ttctctccta tttccttatt
   117541 caaacattag aaacatgcca tgtcaagtaa tattacttga aaatattcat gtaattacat
   117601 tcatattttc cagtaaccag ccttccgaaa ctctttccat gcatatgcct tccgggaacg
   117661 cccggttcag cgattagtga aatctccaac gacacgtctc ggaacaatgc tatgaacctt
   117721 cgcatcgact ttcccatccg gccgcacatc aacctccata aaccatcctg gagcattggt
   117781 ccgctcggga tgagccgctg agaacggtgg attgacaaga tacgtcccct ttggagaaac
   117841 cctgaagctc caccaatgca tgtgcccggc agcatggaag agaagattct ttccgttttc
   117901 cagaatgctt atgacctcct tactgttgga gagcttcatc aaaggaacag tttcttgggg
   117961 gatgagaaag ttgcgatgga tttcgtgatg attcaaggga tggtggctga agaccatttt
   118021 aggcaggtcg tcattgttca tctcatgttc gagccaagcc agttgctcat cggaaaccga
   118081 tccacctacc cctgcaagca tgggatcgag ggaatcgaag aatatgcact tgtatccctt
   118141 gaacaccctt acgaagggta gaatgggatt atgcagcagc ttggcaagct tctgtttccc
   118201 gatatgaacg aagtcgtggt ttccatcgac atggaacacg ggacaggcaa tgcgctcatc
   118261 gagcattcga tggatcgacc gggtgttgtc aatatctctg gtctcatcga catcgataat
   118321 ccgatcgcca agctcgacaa tcatatcggg cttaagttca aggttcacga tatcgacgaa
   118381 acaatcgagc agaccgggaa gctcctcgtt gggggtgtac ttccagatgc attgactcca
   118441 atcctgtccc ggtgaaaaat ggatgtctga aataaaaaca atcttcatat cccccataaa
   118501 tccatccccc attggtcagt catgcagacc ttttgccctc acatatgcaa gcatcgcagc
   118561 gggatcatcg gccatgagaa ttctgctgcc atatgagatc gcatcgtcat acgcttgcat
   118621 atctccataa aacgaaaccg tcagcggcat aacttttatt ctaagcaatt gcttgtacca
   118681 agcggcagga caggggacgg tgtgctgctt gtgaacagac ccgtcagtcc ttagtaccca
   118741 attgagcaca caagccacat cacatagttc ccatggattc gtctgcatgc ttcccatgat
   118801 gtgccacggg aaaaaaccat gatagtggaa cgtatcgcca tgaatcgtgt ggacccactc
   118861 gacaattcgg ccggagaatg aattgatcca tgtcctggat ccgagaccgc gggtggcgag
   118921 catatcggac gtctttcgcg cgcattcgaa agcaaatgct tcgtcgacct catcaggctt
   118981 atccttgatc tcgatattga gcatcaatgc ggggtggtcc accgccaaat ccaacaattc
   119041 ctccaacaac gcaaccggct caaaatcggg gatggaaaca agatcggaag ctattccccg
   119101 taccgcaaca ttcatggagc ggatttcttc ataggtgtgg ttacttattc taccggtccc
   119161 gtcggtcagg tcttcaagat tggggtcgtg catgagaaat aactgcccat ccttcgacat
   119221 ccggacatcc gtctccacca tgtcaacacc gagaccgatt gcatacctga acgcggtcat
   119281 ggtgttttgc atgccatgga cgcaatcacc acgatgagca cacactgcaa cctggttgac
   119341 acagtcgaac atcaactagc cccccaacaa aaattgttcg atcgcggttc cttgacaatg
   119401 cggcatttca agcccaagga gcttggcgaa tgtcgggcca acatccacga taggccttcg
   119461 atcgatcacc acttgtctcc taaaatccgg accgacggca ttgaataccg gttgcggtcc
   119521 gaggtcggga ttgtaaccat gggtagctct accgaatcgg taatcggtca catcgaatgg
   119581 cttgatggca ggccgcatgc aatcgtcact gaacgacgtg tagccatcgc tttcaagcac
   119641 aaaagaaaaa tcgccactga gatgttcgcg cttatctatc tcagccgtcg ttaatacctc
   119701 attgaacccg tagataccct cttgcgccat ctccttgaga agccggtgga ccgcttcata
   119761 ggtgtccgca tcggagggat ttttgagata gacaagattc gacatggcgt tcgagaagca
   119821 gaaggcttcc caatcgacga catcaccaca ttcgttcgtc gtaatgaatc cgtgatctct
   119881 cagatagaca ttcggtttca tgatgcgttt ggtatccatc tgcccgtgat cgctcatgac
   119941 gacgatgttg gtctcttcat atactccagc ccgtctcaag gcatccatca agagtccgaa
   120001 ctgtttgtcg acgaactcaa tccctttgtc ggtctcgctg ttgaacacac catatttgtg
   120061 ccgggtgccg tccatgatgc catcgtgcac gaacatgacc tcgggtgcga attgctcaat
   120121 gatgtcgcat gcacaagcga taaggaaatt atctatgtat ggctggacca tgaaattggt
   120181 tctaccggtc ttgtgatagc tctcgggaag caaatcctcg tgtttgagcg ctatcctgat
   120241 gacctcttcg ctcgatccgg ctcgcctgaa cgacgactcg agagtatccc cagcatatgg
   120301 catccagtat tcattcagca gataatccac atcaggatga ttgccggtaa caggccagaa
   120361 cacggatccc gtgctatatc cggagcgttt ggcgacggtg aacagatcct ccacccgcac
   120421 atgccggtgg tcccacaacc aattctgctc cttgtcgccg gtggtgaatt caaaattgct
   120481 ggctattcca tgcttgttcg gataacaccc ggtaacgatg ctggtgtgga tgggatacgt
   120541 gactgtcgga tagatcgacc ggacatgggt gatttgacaa ctgtctttca tcaccgctcc
   120601 gaaattcggc aatgtcatga gcttgtccac atcctctcgc acaagcgcat ccactgaaaa
   120661 taccaacagt ctcttcttca tattcatatc atcccctcta cgagtcaatc tctgatgctg
   120721 taatggccgt tcgccgatgg cttgccatag atattggtta tctgcctgat atactgggtc
   120781 tgcacctcat aggttccgtc accggttgta gaaacggcaa cggctccatc ttggcacgtg
   120841 tagaacgtca aagccttttg cttgagatga tatctcatcg caacggtcct gttggttatg
   120901 aggctatgtg attgaaccgc taccttagga tccacggtag cgatgaattc gctgttgttg
   120961 gaggtatcat acccgtgatg gttcatcttc atcacgtcgc ttttcagttc atcaccgaat
   121021 cgctcaacca agtctaattc cttggttatg tagaggtctc cggaagtcca gaaactggat
   121081 ttatcaaacg ttatcttcat ggcaagtgac aggtcgttgg cggtcatgta ccccgctttc
   121141 acattttcct ccgccccttc atccgggcca tatactttga ctgacactcg gtcaccaaac
   121201 atgaaagaat ccccggtgta cagtatctcg caagggattt tcagctgcct gactttgtcc
   121261 attgcgttct gataggttgt cgattcatat tcatgtgcat tcttatatat atggccaatc
   121321 ggatattttg aagcgagctc aacgaagctg ccgatatggt cggcatgggg atgggacatg
   121381 acgaaaatat cgatttcctt gacgcccatc ctttgcagtt ggatgtcgat ttcctcaaaa
   121441 ttccaactgt tgctgcagtc aacgaccatg atcagacctt caggagaggt gaacaccgtg
   121501 cagtcaccgg cataggtgac gtcatttgcc gttttcctcg aaccggtggt atgtaaatac
   121561 cgtgccgtaa gcttgcctgc atcctgcgag gtatcaaaca cggccgaagt gtaggtgccg
   121621 tccttatcaa ccggtatttc actggcgtac agtgcggtga tgttgaacag cacacatatg
   121681 acaagcaatg ctgttactaa aaaccgttta agtctcatga tgcctctcct ttgatgaatc
   121741 ttcgttagac atatacatcc acatcaagaa tgggcagggc atatgctctg cccattcgag
   121801 tcgattatgg gtctgtaaaa tctcagttgg ccaggttata cagatcgatt gcttcctgaa
   121861 tctggtcgta tgccgtcttg atcgccttgg cgatggtcgc atcggtggga tcgttgcaga
   121921 ccgtctcaac agcgagcttg ttggcattct tgatctctgc cgagacgccg cagatcggca
   121981 tcagagcatc gcctttgcag tcttccaggc tctgatagac cgtgttgatt ccagggaaga
   122041 tattcttcat gtagttctga tacacttcgc tttcgaaagc atcgacgctc ggagcgaggt
   122101 agccggtcgc tgctgcgaaa tagccggccc attcgccttt gctggcgaac ttcatgaatt
   122161 cataaccacc ctgcatggcc cacttgttac cgttgtcggc gatgaagagc caagttccac
   122221 cggaaggttc accggcacgg gctcccgctt cggttaccga cggacaaggg atcacaccga
   122281 cttcaaacgc attgtccacg gctgtcagca acttcgtcgt catggaaatg acaccgagca
   122341 tcattgcaca gtcttcggct gccatcaaag gaatgatttc cgaactgtag ttcgcaccga
   122401 acgtgacacc cagattctct ttatagagat ctttgtagaa tccgagatac ttgctcaggg
   122461 tcgactcgac gacgccgcca tccttataca agcacttggt gatattgcct tcaagcccgt
   122521 tcctgttgtc gtaggcaatc actccttcac gggcaagagc ggcattcacg tagtaaccgg
   122581 catcggtatg gaacccgatt ccgtactggg catagccacc cttgacaagt tttcggctgg
   122641 catcgagcac tttttcaaag gaagtgaggg aagccggatc gattcctgct ttcgcaaaca
   122701 tatccgcatt gtaataaatg gtgggatagc tcacgccact cggatacccg acgagattgc
   122761 cgtccccatc gcatgatgcc gcatagaaag actcataggt attctcgagt tccggccagc
   122821 ctgctttgtc cttatcgata tacggctgtg cgctcactgc ataatcggcc tcaatgacca
   122881 tcccgatgtt ttcgatggca caatggaaca aagccggcag ctcatttttc agagtcgact
   122941 gcatcttggc gatcaactcg ttctgtgatc cctggtattc gatggtcaca tgataatcgt
   123001 tttgactggc attgaacgca tcaattacct tctggaaata acccacgttg gttttaccat
   123061 gaaatgtcca ccacaccact tctacggttc cggccggctt ggtttcttct acttccacca
   123121 ctttagattc aacctcacct ttcgcaaaca tggggctgag aataagcgcg atgagaatca
   123181 taactaccgt aagctttttc atttttcctt tctcctttat aataatcatt cagcgataaa
   123241 atcgccggga atccattttt agaggcatca acccttgata ccgctcgaga ccatcccctt
   123301 gacgatgtag tcttgaccaa aaataaacgc aatgacggca ggggtcatgg cgaccatcgc
   123361 tgccgcgatg actttcccat actcttccac atcaccggtc acgaggtagg cgacaccgat
   123421 ctgcacggtt cgccagacgt ccttgttcgt tacgagcagc ggccagaaaa acgcattgta
   123481 aatacgaatg aactgataga tcgcaagagc tgaaatcgtc gatacggata gcggcatcgc
   123541 aatcctcagg aaaaagccga tatctccgca accatcaagc gtagccgctt ctttaaaatc
   123601 ttttggcaac tgcatatagt attgacgcat catgaatatg ctggttcccg atatcaatga
   123661 ggtgatgaag agtccggcat aggtgttcac cagtcccatg tgctgcacgg tcacatagtt
   123721 ggtgatgatc acgacctcgc cgggaatcat catcgtgctg aggatgaccg tccataggaa
   123781 ttttttcagc gggaacttga aaaacacgaa cccgtatgcc gcaaggctgc cgaagacgat
   123841 ctgcgagacg atggccacta cacagacgat gatggagttt ttcagataat gaaagatggg
   123901 gacatcggtg aaaactttag catagttttc cagtgtcggc actttaggaa acaggcgcaa
   123961 cggtaattgt gaaagctccg cctcggtatg gatggaaaac atcaggccga tgatcagcgg
   124021 agagatgaat gccaatccga taagcaattt tgccgaatag gacacaaccg acaggatgtt
   124081 tttctttgtt cgtggattga tcattggtaa tgcaccacct ttttttcaag agcgaattga
   124141 attctcgtga cgatgaagat caataggaac aagcaaatcg cctgtatgca tgccgtttca
   124201 aaccttccct ttatcaaggc attctcgtag atataataga taagcgtctt ggtagagttg
   124261 acaggtcctc ctccggtgag cagccgaatc tgggcaaagc ttttgaaagc gctgttgata
   124321 ttcagaaaaa gcacgaaaaa gatttgagga gaggccatcg gaatcatgat gtgtatgatt
   124381 ttttgcatat tgttggcgcc gtccagaaca gcgctttcga gcagttcagt ggaaacattc
   124441 ctgaatccga ccagcaagaa aatgaagctg gcgccgatat gcatccagac agttacgaaa
   124501 cagacgaccc aaatggcagt tcgcaaatca tgctgccatg cgatagtcgt acccaaaatg
   124561 ctgttcaaca agccgttctt ctggcggaag ataaaaacga agattccggc agccggagct
   124621 gaagcgatcg ccattggcag ggcaaacatc gtctgataga ttttggaacc tttgactatg
   124681 ttcgcagaaa gcagggcgaa gaagagagca aacatgaaag tgccgaccag gttgatggcg
   124741 gcaaccttaa gggtcacccc cattacggtc cagaaaacct cgctggtaaa caccctcttc
   124801 cacatcctaa gtcccacaaa tttcgtagga tttccatatg tattggtcaa ggtaaaggaa
   124861 agcacgatcg ttcgcaggaa cggataaatc gtgaacattc cgaacaatat catcgcaggg
   124921 gcaataaaca gatacggtaa aggtgaaacg cgtttcttaa gtccacaagg agcgtaacct
   124981 gcatttcttc taaacatcac taggtgcctc ctatgattga agcatgttga gagccagctc
   125041 gacagagggg aacactcttt tgtgatagag tcgttcctgc attgatgtca acaacctgtc
   125101 gccgtattga cctgatactt gtgctatcat cccaaaacct tcaatataaa atgtaattac
   125161 aatctaatgt ttattatcat acatcaaaaa aacacagaat tggaaacaat tttctgaaaa
   125221 atcttgtaca aatatcataa tacaatggcc ttacgggtca gtcaagaaaa attcttgtaa
   125281 aatataattg taattacatt tcttaataat tctgctttct ttttctttaa atcctaggta
   125341 ttatgtagtt acataaagaa gagagggtat tatgggactc acactcaacg acatagcaaa
   125401 gaaaatcgga gtatcaacag caacaatctc acgagtcatc aacaatgaca acaacgtgcg
   125461 cgaagagact cgcctgaagg ttgagaaagc gctggctgat tacgactaca agtatcggcc
   125521 aagaagaaaa tctaccaagc gaagcaacgc cgacgcggtg atggtcatcg ccggacaact
   125581 ctcgaatccg attacgctcg cctatatcaa aggcataaga gatggattgg aggatactcc
   125641 ctataaagtg ttcgtttcga tcaccgatta tgagacaaaa aatgaagtcg attatctgca
   125701 atatgcgaag gacaactctt ttgcaggaat tttcatgctc aatgtcattg agaatgaatc
   125761 cctcatccga acactcaatt caatcgaatc gccggtaata ctggtcaatc gctatctacg
   125821 gtccaaggat accgacatcg tgaccgtcga caattaccga tgtggattca tttccacgca
   125881 gtatttggtc gaccatggcc atacccgcat agcccatatc gccggccctt cgaactccat
   125941 aacatgccaa aacagaaccc tcggttacat ggatgcaata aatgaaaaca agcttaaatt
   126001 cagaacccaa gacgtatttt acggtgaccg cacctaccaa agcggttacg aatttggtca
   126061 gcttttctgc aagatcaaag ctgaagaacg tcccacggcg gtattttcaa tatcaggaac
   126121 catggcggag gggtttgccg acgcggtaat tcaagaaggt ctttcgattc ccgaagatgt
   126181 ctcattgatt tgcaacgatg attcaagcaa agaatatgta cgtaagctca agatcacctg
   126241 cgtcgagccc aacatgttga ccatcggtat ggcagctgca gaattattgc tcgaacgaat
   126301 caagaatccc aattcccaat tcaggagaat cgtgtatccg ccggttctaa ctgagaacgg
   126361 ctcggtcaaa acactcgtcc aacaggaagc cgtatcgagc tgagaacaac accctgctct
   126421 ttcgtagatt gaattttaag ccataagacc tctctccaag taatcatttc ctgaagaagt
   126481 cttcaatgca tgcaatcatg atagcttcct tttccgagtc aactatttgg acttctgttc
   126541 catctaacat aaaacatttc ccatcaaaaa aagatttcac atttggcatc atttggtaaa
   126601 gctcgctcaa tgatagattc tccaagccca gaaattatgg atgatcgaac cttttcagga
   126661 gctaagccag tagtcaataa ttcatattgt ccaacataaa aagaaaccac cgaaatcttg
   126721 aaaattgcat tcgaaataat tgataacatc atcgataatc ttaatctcat gaatcgcatt
   126781 caattttcga tgcaaagcat cgtaatcctc agtgcactca gtcagtatgt gcattcctac
   126841 ccattcattc attaacctgc atcaaaaggt cgtacttatc ctttcatcgg aaggttttcc
   126901 cccttcagag agtatctttg ttccaaacac aacaactctt ccatggttaa ttaaaacctg
   126961 aatcatcaat tttgagcgtt ctacagtttt cagttactgt tcggatggta tctaaggaaa
   127021 cctccttgtc aaaatcaacc tcaacatgca aacgtatttc aacatttgct ccatcaatgg
   127081 ataaaagatg attaagaatc tcttcattga tttttccaat ctctcgaata tacctcggag
   127141 tatccagttt agttgataag tgaaaacttt tcggtccgga agattggctt gatgctggag
   127201 tgtccgccgg aaaactctca tccggtgaaa gcacaaaatc ttcttcggta tcagtcgaaa
   127261 ctatctgaga ctcagtcttg tcttttactg cagcgatcgc ttcagcatat cgaatgatat
   127321 agtgggaatc ctctacagac tcgacctttt tctttatttt cagaccataa tatgtcccct
   127381 cttctgaaat gccatcagcg agcccaaaat actcatcccc ttcaacgcct tgggcaatag
   127441 catctgccaa tacattaaaa ttcaacaatc ttggcagata gagatgagta gcgaacatat
   127501 cccataagtc ttttactctg ataaaggatt tttcttttaa aaaccacttg ttcagctcca
   127561 tcatcaaaag tggtggtgcc catttggcga taatctcttc atcctgaacc attctcctgt
   127621 atgcacgggt agcaattgat tctcgagctc cgccaattcg gagacaatcc cactgaacag
   127681 tttttttgtc atgctctaca tcaatgccgg gaacaaacag ccaagaatac gcctcatgta
   127741 tcctgagtga aatggtgtcg tcatgtcgtt tcaggttacg ttgaacttcc ttgatttgag
   127801 tttgatcaag gttgatttct tctgtatctt cctcaatgga cttccaagcc aaaaacattt
   127861 tcacctcttc aaaaagattc attttgattg atgaatcggc cgctgcaaac accagcatgt
   127921 ttttgaacat tcggggagat tctccccgag tttcgagaat cttttgagct tccaccaatg
   127981 cactgctttc ttcgttaccc tgattgtgta tcttggtagg atgaagcacg acaagacgga
   128041 cgctcttctc atcgggaaca tcaagggatg aggaaggcca tatgtgaatg cccttaaacg
   128101 actcagctct ttgagaagaa cgaatcctgt tttcaacttc gtctaacaca tcctgagtat
   128161 tcaacaaggt tgctcgatca gctgccgtct tttgcaacgt aggccttgta tcgtagtaga
   128221 accgagtggc attagtgttt gaatagagat aggtcaacga gtcccgcaag gttgtaagcg
   128281 catcattaaa gaccgcaatg tgctcaccgg gctgaataac gcccaatcga atcctcgttg
   128341 tttcgatgcc cctgagattc atctgcttca cggaaggagc acttccaagg aatatggtgc
   128401 gagcaactct tcgtgcagcg atatgcttcc caaacctctc tatttctttg tctttcaaaa
   128461 acggtaccga ctttgttcca tcgacatctc gttcgatgat tgcattccaa ctgtcggaca
   128521 gatgtcgaga taattcatct cgaacctgtt ggacatcaag aggaaacgaa gatggcatga
   128581 tcatcaaact ttggtctccg cggatccaca aatcatagat aacggccgcc atcaatcgca
   128641 acacgcctcg agtacgctgg aatctttcca aggtcgccca atcttcatac agacgatcaa
   128701 aaacttcagg atggattgga tagcaactga tcaatctgtt tcgatattcc aactccttcg
   128761 tctcaattgg gaaatcggaa gcgttttgaa tgtacatatt gctgaaagcg ttacacacgg
   128821 catctcgttc gccctcatta ctgcaaggag agaagagtct tcgcctgaca acttcaaacc
   128881 cttcatgagc cgcaaccggc ttccaaatcg actccatccg tccgaaggtg tgttcaattg
   128941 tttccaagac cctttgtcca gcctcgccac ctatttcaag ttccgattcc ggcagtgatg
   129001 ctatcaaaac ggagttttta ctgcttcgta ccgcttctgt aagctcttgg ataaaggtga
   129061 ttaaattgtc aaagcttccg gcgattaagc catccacccc atacagcttt tttgcatacg
   129121 ccacaagctc atcaatcaaa atcatgcacg gaccgacagc atcaaacatc cctcggaggg
   129181 catttgatcc gggagagacg cctttattgt cagctttctt aatgaaatta tacaggtctt
   129241 ttttgcctga ggattcggtc agttgagctg caatctcacc ccacaacgta ttgatggtaa
   129301 ttcccggcat agtaggcggt ctgcgagcct ttgttggatc aagcgctgtt cctacaacca
   129361 ctgcaacatg gactttgggc aaggtagtaa cttgagctgc ttcaagcact gctttggcat
   129421 tcggtagctt atcaaccgga acagtgcctc gaagcaaatg atacaacgcc agcatgctgt
   129481 gcgtttttcc gccgccaaat gcagttttaa gttgaataac cggctctcca tccttgccgg
   129541 aaacccgctt gatcgcttca gccaataagc ccttcatccc ctctgtaaca tatgtacggc
   129601 taaagaattc caccgggtca cgatactcgg gcgacccttc tcccctggct acttgagata
   129661 aatccgcggc aaactcggcc ttttgatatc tcccttgggc aacatccgga tgcggttgta
   129721 ttacctcacg ccaaggcttg agccccttaa taggagcttc ccgcatgatt ccggcattct
   129781 tgggaattgc cttttggact tcttcgggtt gaaccactga ttgagccgaa ccttccggag
   129841 atccatacag cacagtacga gccaactttc gaatttcctc aatatttgtt gtgtcaatcg
   129901 gctcaagaag ccgtgtcatg gtatccaatg ccctccacgt atactcatta tcaaaatctt
   129961 cccgacctgc atgagcccag gcatgtcgta catcgattaa ttcattgatc cagcttctct
   130021 gctctttagc cagcttcttg ttaaagatat ctttccactg ccagttgatg agctgtaaac
   130081 accggagaat atccaaagag ttttccaact cgtcatcgct tccggtggtg ggtaaatctt
   130141 ttttttgatc gtaagtaaaa ctgtcaagaa cacccaattc ccaccaatcc gatccatagt
   130201 aagctttgag ttcacaaacc acaaatggag ccaaggttaa taaggtatat cgaaatcctc
   130261 tattcaacat ttcattgttt gtcatcattc cctcccctaa ccaaaatcaa attcgcctgg
   130321 tatatattct ctagctttat ttgctctatc ttggattgtc atccaagagg ataccaacga
   130381 attgtacgca tatgcttcac cggcccagcc tttccgttcc gcaatcgtgt ataaccgata
   130441 cgcaagtgcc tttatattct caacaagata accggataat tgagccatga ttcttgtcgc
   130501 accctcaaca ccatcctttt gaaatgcgag gacaacctgc tgggttgtca accagacgtt
   130561 ataatcaatt cctatcttaa atcccggtaa atcctttctt tcgttgagat acacgtcccc
   130621 tttttgcgct gttattatcc ctttactaac cagctgatcg attgaaatgt tcttagcttt
   130681 tgacagcact tcagcagtac caaatttaac tgggttaaaa gcatattgtg agaacagatc
   130741 aacgcagaat ctgctctcgg catccatttc tccttcttgt tcgctgaaga actcatcaat
   130801 catctgattg ataatctcca gtgcggaacg aaccgtcatc tccgttccat cagcttccag
   130861 aatcttgcca ttacgagaaa acacggccat acccggaccg attgctgatt gagccaagtc
   130921 aacaggtgca atattggctt gctgtagttt gacaagagca ggattcaact cccttttcag
   130981 tgttgtgaca aattctttgc gagtgataaa ggaagatgag gggttacgct tcctgcaaac
   131041 caacacaatg gaagaggcaa gcgcgttcgt cccctgagca acagatcgca cacttctttc
   131101 agttcttatt ggccaggttc cggttattgc aaatcccgct tggattacag ctgaaagcat
   131161 agtctcccaa ccagtggaaa ctgttacact cgaggaatta tcgtcatcct cttctccact
   131221 gcttatctca ctttgcttga atgcatagta aatggtcaca ggtatatccg ctcgagtata
   131281 cttatatact tgacggaaag tttgcagcat tccgtcctcg aaaaagtctt ttgctttttc
   131341 cttgtttcca ttgaatctat agggagtagc taccaattct tcttctttgg gaacaagcat
   131401 agtgctaaat aatttaggat atgtatcctt caatgattgt cgcatccaaa tgtagaaaaa
   131461 atctgaaaga tctgcatatc cgatattatc gtaatatggt ggatctgtag aaatcataat
   131521 atttctaaga ccattgtcct tggctgcgtc gtgctgaatg actgatcctg atagcatagc
   131581 aggaagagtt tgaatacttt tcgttagcca catcaacata ctttttaatg atccagctga
   131641 attgctaaaa acatttgcct ctgcataatc ccacaccata gggattgcat gccgagcaaa
   131701 tgtatttctg attgcactca atgtatttat ccacgaacaa atcgttgagt tgtaatcggc
   131761 aagcttgtca actaaaaagg caagatatac ccctattgct tctccataag cttttgcccc
   131821 tttaccacca tttcgaagtt cgagtccatc ttccattccc ctatgtaaag catcttcata
   131881 agccttttgt tgggcttcag aaaccaagtt gctgaaggtg gttaaagcag tgagttggcg
   131941 attagtgaat aagtctgccg tttcatccat tccatacaga ggaacactta ctcttgcttt
   132001 acctgataat tttccagaag ggaaatcctc cggtcgatta atttcagcaa tttgggagtg
   132061 atcctttgat ggtgacagat atagacgagt atttgagccc tctgccgcta ttgccagaag
   132121 ttgggatcca agtccttcat gctgagcaaa ttccctgata tatggaagat ctacaaaggt
   132181 tccacaacag ggacaagtgg ctcctgttcg ggtcattgta ccttttagca cctttgcttt
   132241 tccgcttttc actttatacg taactatgtt gtcttgtatt tccagcacta catggtgttc
   132301 ttttcccttt tttgtcgaga gatcaaacga acgcacaagc ggcatctcac acccacacgc
   132361 agggtttggg catgtgactg ttctggccca aatccatgcg atgactgttg cttcagagcc
   132421 attggatagc tttgctgttg gatagagatg accgattcgc ttgaaggctt cctctctcat
   132481 ccattcacca tagtatctga catcttcagc aagacctgtt gagcctttcc atgcaagttc
   132541 tgatcctaac ttggctctgg cttccgggtt gacaggcggc ataccttgga atttgggagg
   132601 gatctcaatc atcgccttgt taatcatcac tgcaacggga ttgagatcag aagcgtgagc
   132661 tttgagcccc aggcgctggg cttccaaggg gatagcgcca ccgccggcaa agggatcaag
   132721 gaattcgggt acatcaccgt cggtggaacg ttcaatctcg gctttggctt cagcaagaag
   132781 ttcttcatca ttggagtttt cccaaacaac aaggcgttca agaatatcaa aaagcctttt
   132841 gcgttccttt tcctgggctt cttccgttgg gaatttgtcc ggatgtgacg atggatcatc
   132901 aacaagggaa gagaagatga cagcccgtgc tgcggccaat ggccgacgag cccaccacag
   132961 atgaagggta gacggatgac cgtgacggat tgatttttct cgaacagacg cattgttgat
   133021 tgcatctaat ggaagggcta cttctatcag cttctttttg accattctgg aacacctctt
   133081 tgttggctaa atataacttg tgattgtgta acaagct
//
LOCUS       DAOSLI010000002        72429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_417_length_72429_cov_26.892309, whole genome shotgun sequence.
ACCESSION   DAOSLI010000002 DAOSLI010000000
VERSION     DAOSLI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 72429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72429
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_417_length_72429_cov_26.892309"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..332)
                     /locus_tag="PLF67_00620"
     CDS             complement(27..332)
                     /locus_tag="PLF67_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOE88545.1"
                     /translation="MHSSVRAFLKAQAHSLKPIVMVGKGGLSEQVVRALDEALSSHEL
                     VKVKFQAHKDELGPIAESLAERTKSDLVSIIGFIATFYRASDEHLIRIPLELSAKNQ"
     gene            complement(337..2079)
                     /locus_tag="PLF67_00625"
     CDS             complement(337..2079)
                     /locus_tag="PLF67_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE88546.1"
                     /translation="MSKKKHHAKPWDFLDEYRGKEFTGTWPTVVEMFDITVKRYPDNE
                     SFIAFTPKKEAYTYAEAYEHIQTIASYLVVEGVTKGTKVALTGKNSPQWAMSYLGILY
                     AGGTVVPLDASLSDEDMIKLMAFAGVKIFIADSERLKGFDEKDTLGLTHRLSLEPSEE
                     FPMIMEQKGDRRRKAYKATVEETAAILFTSGTTGTPKGVMLSHENLVSDCYLAQGNMW
                     IYPTDVFYAILPLHHSYTMMAVFIEAISVGASIIFGKKLVISQVLKEMREGKVTMFLA
                     VPMLFNKMIAALMNGVKEKGAVLYGVIRFLMGVSGVLKKVFKVNIGKKMFGFLLKKLS
                     LEHNRICISGGGPLPASTFKLFNQLGIDFVQGYGLTETSPITHLNPVEAYIETSVGKN
                     LPQVEVRIVDPDSDGNGLIHIKGPMVMQGYYKNPEATDEVLKDGWLNTGDVGYQDANG
                     YLYLTGRAKNLIVTEGGKNVFPEEIEDHFQLYDEIDTICVLGYIVDKQKKSEGIRALI
                     YPSEKYSDEMKKTHGEQSAAEIEKRLNQIVGEVNKSLQSYKKITRVTVVNEPLEMTST
                     KKVKRHVVAQKYKD"
     gene            2224..3558
                     /locus_tag="PLF67_00630"
     CDS             2224..3558
                     /locus_tag="PLF67_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOE88547.1"
                     /translation="MELKCFADVVAYLEGFLNLEKKTQHYTTREYRLDRMIALLDHVG
                     NPQESFQSIHLAGSKGKGSTASFIASGLTAMGYKTGLYMSPHLVDYRERFTLSGTYFS
                     DEALVRAGNLLKTSVGDFSFSDEWGETTPTTFELLTTYAYLLFKEEKCEWAVVETGLG
                     GRLDATNTLQSSASVLCPIELEHTRILGSTIGEIASEKAKIIKAGQPVFIGLVPEEAK
                     EVFLKECRKQRAKPFLLEELLAGYETRTTLEGERVTLAWSDGTEDRLLLSMKGSIQAQ
                     NVALALLTLRTLGLFGGRTIHALEANTVPGRFQQVAPSLYIDGAHTPASLSALTRSFD
                     ELYPSKDANVVIYGALEDKDHLHMAPIILQHFNKIIISRPGTFKKSDLQKLFSIFEAL
                     KAESRSTASIFLIEDNSQALEKAYMLKGEDGAILVCGSFYLGGGIAEALKRR"
     gene            3555..4964
                     /locus_tag="PLF67_00635"
     CDS             3555..4964
                     /locus_tag="PLF67_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOE88548.1"
                     /translation="MSLNWREIELIVSELPLKGSRLQGVTQHDFNAISFHFFHPVHRQ
                     WTLYTEVGTPSSRLHKLTRARAKLPKTQRFVQFLKANLVNCLVSEVAQTPGERFVDLT
                     CTRGEDTMHLLLRFYSGAGANIIVTDDSYTILDLLLRRPRRGEASGLSFALPEPKEID
                     GAFPIRRRTKESFNEQIEEEYAAERNIRTLESLYERATLLYEKKARSLEHTLASLNTR
                     IDQTRDFERYKHLGDLLSSNQHLIREGQTSLSVHDWETDREVEIPLDPTRSPRDAIQL
                     YYESYQRSKGRYENALGELKRVQEELRAHQAIFERALDRSLPQEEALALLTSLVEESE
                     KEPIVRPSVGLVFQSGAYTLLVGRNAKENDELLRRHTRGNDWWVHTRDVPGGYVFIKY
                     IKDKPPPLEVLLDAAYLAILYSKAKKAGKAELYYTQVKYLRRAKGAKMGTVLPTQERN
                     LSVTLDEARLNVLLAQGEV"
     gene            4964..5773
                     /gene="amrB"
                     /locus_tag="PLF67_00640"
     CDS             4964..5773
                     /gene="amrB"
                     /locus_tag="PLF67_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOE88549.1"
                     /translation="MTTYHHTIFYPEEREPLDEATYKRSEAGTLKSLPSALLLPHAAY
                     SMIGEALHQTFAHASSLKPELVVFLGPLHQPVLDRDKPAFLFAPAADTFEQAGRTYQF
                     DEAMIGELTLGFPIRRAASYYEEEPAFELTLPFIDSYLAPNAILPLIGKVEDSNQIAQ
                     LILALKTIVQRQPKVLFVLSANASELLPQREATDEANHFIEDLIHEHQSLGIRSSCNW
                     GMIEAMNRSALVKGRWKIASAFLKGTDHGRITDTKNESGLMTWHIGAYKGV"
     gene            5778..7250
                     /locus_tag="PLF67_00645"
     CDS             5778..7250
                     /locus_tag="PLF67_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88550.1"
                     /translation="MKLYQPFNTTYNDEEITAIPFASAAVELPAFHLQGERAASHLYK
                     DGKLTAWYWQGIALIENQRSVYFPRQNLFSFTELATVRRSKALSWVRRLAKALTLTTG
                     SFLDLSSGILPLWRIYGGEDGSILILSQDLGDLFAAMANDEPKFFNISAWVHHNIHPA
                     FTLCDQLTQLLYYAITGTPPFLRKETREDRYRHLPLAYSFEEVSLQTRLFIDASLSLS
                     LTKQRDSTGNKEPQKALTAFLDATESIEWDAENRTEVPPPSAWQNTPKIQEFLASQAK
                     RAKRIVFWRMKGWLIITIAVSVILVSWFTIDRVSEALKPPYTQFMDPPAIITEYYQGL
                     NALDLSHMDAALAKKVKNPWTMEVTNLFVTRQTRKAYEGFSPTVDPNEWFAGGQKPLI
                     EGSFLYGTTDVTVTRLDERTFTAQGTLYTPYPYDEEEADQEHRQSGSYMYTLTQTFTL
                     DVGKKGWYEITSISSPRITRIGFLEIESIPRLEQTPPPAR"
     gene            complement(7210..8208)
                     /gene="miaA"
                     /locus_tag="PLF67_00650"
     CDS             complement(7210..8208)
                     /gene="miaA"
                     /locus_tag="PLF67_00650"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOE88551.1"
                     /translation="MAKLSLKSSAEPLPGRSSSKSPTTSETIKPIIILFGPTAVGKTA
                     ILSRFDSARYSVINADSIQVYKGLDIGSAKATGAEREAIEHHLIDIREMDQSFSVGDF
                     IEAADEAVALIHSQNKIPIISGGTAFYVKHFLYGLSEAPSSNEDVRARVGALIEEKGL
                     SWGYKRLAEVDPVSADRIHPNDTYRISRALEVYEQSGKPLSSFDLPSEKRISNPILII
                     GLERERQELLDRLKRRIKQMETDGLVEEIRSLLRQGAQSTWASMAGIGYREFFELSES
                     GEYSVQSVLRRIERNTKLYTKRQMTFFRSFAAAEWFGADDVDSIIERVEAFVRGEG"
     gene            complement(8132..8335)
                     /locus_tag="PLF67_00655"
     CDS             complement(8132..8335)
                     /locus_tag="PLF67_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88552.1"
                     /translation="MSIPLDSLIDYEDNIYRITCVAIKEANILSNPGLGGKEIEENNG
                     KIVTEVLSRALAGEILFEEPDDL"
     gene            complement(8417..8941)
                     /locus_tag="PLF67_00660"
     CDS             complement(8417..8941)
                     /locus_tag="PLF67_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE88553.1"
                     /translation="MRIAISGKSGCGNTTVSSLVSKELGYPLINFTFRNLAEERHMEF
                     WDLAALAEKDDSIDFELDRRQVEMALAQKDCILASRLAIWMLKEADLKIYLDATVEER
                     ARRIHKREGGTLEEKIAETTARDAKDTARYKRLYDIDNTDSTVADILIDTTRKSAAEV
                     AHIIVHEVRSREKG"
     gene            complement(8952..9812)
                     /locus_tag="PLF67_00665"
     CDS             complement(8952..9812)
                     /locus_tag="PLF67_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOE88554.1"
                     /translation="MTGYGYAEKAHSVFSLSVEIKSYNNRYLEIICNLPPFLSGYELE
                     ISNMVSEKAARGRVEVFVKVKTLKSDLQVVVDKEVVERLSEAFARIGAISGKALKPAL
                     SDYLSQEGVLVSISERDGELYREDLFAALDEALNQFALAKEREGAATALHLTELGKEI
                     GASLAVIEAGADRLEEHIKQNLLKRFDELLGDKQFDENRILQEVAVMLVRYSAAEEIS
                     RLGIHLKEYHSLLQTKEPVGKRLDFLCQEMNREINTIGSKSQLVELNLEVVKMKNSLE
                     NIREQVRNIE"
     gene            complement(9818..11185)
                     /locus_tag="PLF67_00670"
     CDS             complement(9818..11185)
                     /locus_tag="PLF67_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOE88555.1"
                     /translation="MNLPFRRIYLVGIKGSGMARLALLLANGGCRIGGCDTQEDFFTG
                     RSLTEAGIAADLGFDRSNLNGTWDAVIHSAAYPPSTDILIHAKERGIPLYSYPEFLAL
                     LSTLRPTYLVIGTHGKTTTCAMADHLLGEEGGYWAIYGSTLLKRSNSSGKKSRFFLFE
                     GCEYQDHFLLYRTSGAVLLSVEYDHPDYFGDLADVHDSFRRFVDGIEQGGFFIYNNDQ
                     TGSRAIGEYAKARRTDLLVLSFGFDPESDYPIVEEGKQHYRIGGISTRLTVGTPELVT
                     DHLGALLLASMIPEKRIDPVRMKGLAMRLATFGGVVGRLEYMGERAGILFFDDYAHHP
                     SEIIVSLREMRTRFPGRKILVLFMPHTATRTHALLEEFAAALRGADQLILQPTYASAR
                     KDEETDPLALYALLKADTDCTYAADDEEAVAQALMRLKEGWLCITMGAGNNRALNSRF
                     LRSVP"
     gene            complement(11255..11431)
                     /locus_tag="PLF67_00675"
     CDS             complement(11255..11431)
                     /locus_tag="PLF67_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88556.1"
                     /translation="MSKAHRGSGIRDKYKNGRGTCPKCNRTGVKVLYEATLEGAKTKV
                     CKPCNANLKKKASA"
     gene            complement(11514..12539)
                     /locus_tag="PLF67_00680"
     CDS             complement(11514..12539)
                     /locus_tag="PLF67_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88557.1"
                     /translation="MKRLVTITVIFLLVSLSLTAKSTTQTMWAFGGARFALGSDSDSF
                     LGNPALLASSDRTTRSFILSSGFEDSAESWDFEEPSVNLGVSFIAGRMAFSIMNVSSV
                     TRIKEYTYTGKRTGLFQFDWAFGRQPFYIGFTAQIEALSERSEMTFRPELIWSDYFVA
                     TNFSRYEPVDQIGSISFGVSFLLDYSWISMAVTSTKFASSETSETLNISFDSLLKSLG
                     WGMAVETPKYNERNELHLFTVRAAVDIANMGSSEERELRMGTSVTLNLLPTYDISLIL
                     GYHEQKGEATDWLGLKFAQGFQNQTLAITLNTMKILIGYEFPTAWYVNKPSPRPAKAL
                     VGLRLFL"
     gene            12614..14065
                     /gene="murE"
                     /locus_tag="PLF67_00685"
     CDS             12614..14065
                     /gene="murE"
                     /locus_tag="PLF67_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HOE88558.1"
                     /translation="MKALGELLRSAHIPVTSTQQSFIVTSLCTHSNACREGSIFFGFR
                     GQNSDGSLYAKEALQNGAILAIIDEEASVEDHQFIIRVKHPHKTFALMNAAFHDNPQA
                     ELKLIAVSGTDGKSSTADFLHQLLSFEGIPAGLFSTTSIDDGAGKIPSPYRISTPEAD
                     LLYSFLDRCRANRMEYVIIEATSHALSEEYSRLYGLTFEIGIITTLSGDHIEFHGSRE
                     AYLEAKLSLLGMIRPRGHFITSVENRELDRCLKTLDSSIHPHILGLDHPYTIQTCDLH
                     SVRGTFEGRPFTVHLGLEVFATNALLALLASSVITAKNPVELLPHLSNVRPLKGRMQA
                     VPNDKGVLVFIDYAHTIGAYEHLFAFIKQVRGTSRVITVMGAAGGRDKKKRSVLGGIA
                     SKESDILILTEEDPVFEELSAINGMIRNGIASKSCRVLEIDDRKEAIGEAIRLAQAGD
                     IVLLLGKGHEQSIQRRNRTIPWDEEQAVLEVIR"
     gene            14062..15120
                     /locus_tag="PLF67_00690"
     CDS             14062..15120
                     /locus_tag="PLF67_00690"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOE88559.1"
                     /translation="MNVALIYGGRSAEHEVSINSARTIERILKRNHHTLYPIAIDRNG
                     CWFLQTRVQNTIDEEIPLWIVPGSGIYAQSGMLPIDVGFPTTHGRGGEDGNLQGLCTL
                     AKIPLAGCDTVSSAIGMHKALAQSVFDRAGIPTIPTIIVTEAMPVTDSLLEELRQSLG
                     SSLFVKPENAGSSVGITVLIEADLPSLARALRLALSYSERALVQPYYPEVIEAECAVF
                     EDPEQGIIACGPGMIKNPKKETFLSYSQKYTLQGGAYLELPSGLSDEVEEEIRSLAVR
                     AFEAIKGNGYARVDFFLIDERPILNEINTLPGLTATSHWPKLLQYGGYSIERVVDLLL
                     EQAISDFDRRWNLRTSHD"
     gene            15113..16300
                     /gene="dinB"
                     /locus_tag="PLF67_00695"
     CDS             15113..16300
                     /gene="dinB"
                     /locus_tag="PLF67_00695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOE88560.1"
                     /translation="MIETVIFHVDIDAFFAAVEILDNPDLAGLPLIVGGFSKRGVVTT
                     ASYEARKYGVHSAMPMIQALRLCPHATVVPTRRTRYSEVSARVMGILREFSSDVHQIS
                     IDEAFLDMSGTERLWGRPREAALKLKERVHEETGMTISVGIGPNRLIAKMASDYNKPD
                     GLCKVSESKKELFIDAVGLSKLWGVGKATQEELKRHRITTTGELRAIPIERMKKLFGN
                     SMGSFLYHIVRGIDPGIFSEAKSRSISTEHTFLEDVGDREVLEETLLSMSHEVMFRSL
                     DERKIARTVGVKIRLSDFSTSTVQTTPAEAIFSAEQVYAIAKELLARKWRSGMKVRLI
                     GVGLYQLYDGASPIQGELFESEHEKKRKLEEVVLALQKEGHVLIKAANLGKHPLGDED
                     QQG"
     gene            complement(16259..17077)
                     /locus_tag="PLF67_00700"
     CDS             complement(16259..17077)
                     /locus_tag="PLF67_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOE88561.1"
                     /translation="MTDIISSILLGIVQGITEWLPISSTAHLLLAEPYLPLALSASAK
                     EFFFVGIQFFSILAVGVLYFKDLSPFTRDKTKQLASLNLWGKIIVATIPAGVMGVLFD
                     DMIVARLQGPMVIASALAFYGLLYIILEKRHARRTSFRVTDSIESLTYRHAFLLGLFQ
                     VLALIPGTSRSGSTVLGGLIIGLSRPLSANFSFFMAIPVIAGATLLKMLKLGMSYSAN
                     EWMIMLTGGGTAFIISLFVMKRLRSFLKTHTFIGFGYYRIALALLILIAEGVFA"
     gene            17170..17670
                     /locus_tag="PLF67_00705"
     CDS             17170..17670
                     /locus_tag="PLF67_00705"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOE88562.1"
                     /translation="MNIVLVEPEIPQNTGNISRTCAATHTSLHLVHPLGFTITEKHVK
                     RAGLDYWKYLELVEYPDIAAFFDQHEKKELYYVTTKAERTYADIAYGDECFFLFGKET
                     LGLDEELLAKNRSRCIRLPMVREARSLNLSNAVAVIVYEYYRQKGFETLVKAGALHHR
                     QWESQA"
     gene            17667..18473
                     /gene="proC"
                     /locus_tag="PLF67_00710"
     CDS             17667..18473
                     /gene="proC"
                     /locus_tag="PLF67_00710"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOE88563.1"
                     /translation="MRKVGMIGCGVMGSAIARTLQDEGFVYDIDPERARALADGTRFQ
                     VSDSLETVVQECNLLILAVKPQTLSSIYPVLRQAGSKEKRWISIAAGVSTSTLAENLG
                     TTEVVRYMPNIAARYKQSVTAVAAHPEAGRELLEEAKTIALGFGRVHVLDERLFDGFT
                     AISGSAVAFIIKFLDSLAQGGADEGLAYPLGLTIAAETAESAVTLIEESGLQPRDLIN
                     RVSSAGGTTVEGIKALNDGNLQATVMDAVRKTAQRSRELEEAAANHTERK"
     gene            18481..19755
                     /gene="serS"
                     /locus_tag="PLF67_00715"
     CDS             18481..19755
                     /gene="serS"
                     /locus_tag="PLF67_00715"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOE88564.1"
                     /translation="MIDLRELKSRYDEIITNIKNRSMKIDLDAIIALQDQRSSLLQEV
                     ESLRYERNENAQKMKGKLDQETRARLIEEGKNLKAKIAETEERFNRVEQEYLLLGRQI
                     PNYAHPSAPVGKEDTDNLILKRVGTPPTFAFKPLDHVELGEKLDLIDFEAGTKVTGPK
                     FYFLKNEAVILQMALERYALDILIKHGFAPYITPDIAKEEILEGIGFNPRGPESNIYT
                     LEGTGTALVGTAEITLGGYHAEEILDINRLPIKMAGLSHCFRREAGSAGQFSKGLYRV
                     HQFSKVEMFIYCLPEESDAIHEQLLAIEEEIFSGLGLAYQVVDTCTGDLGAPAYRKFD
                     IEAWMPGRGENGEYGEVTSTSNCTDYQSRSLRVRYKDADGQNQYVHTLNGTAVATSRA
                     IVAILENFQEEDGSIRIPAVLVPYTGFDRIGG"
     gene            19759..21213
                     /locus_tag="PLF67_00720"
     CDS             19759..21213
                     /locus_tag="PLF67_00720"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOE88565.1"
                     /translation="MFHRSALSTDFYELTMAQGYFLERNNPNVVFEMFYRTNPFGGGY
                     VLFAGLHDLIDKLEAFRFSPEDIAYLSGLGIFTKEFLEYLSKYRFTGDIYSVEEGTVV
                     FPGEPLMRVHTTMIDASLIEGILLNTLNFQSLIATKASRVSLAAKRGSVFEFGLRRAQ
                     GPDGALSASRAAFIGGCQFTSHTLAGKLYDIPVSGTMAHSWVMAFDSELESFRAYAEL
                     YPGSTVLLIDTYDTLGSGIENAIIVGLEEKEKGNTIGVRIDSGDLSYLPRVIRKRLDD
                     AGLEDAFIVVSNDLTEEIIQTLVHDGVPIDSWGIGTHLVTGGIQASLNGVYKLAAKEL
                     RDGSYLPTMKISNSFEKTTNPGIKQLYRFYDAEGGAIADLVTCFDEEISVGNDYVFYH
                     PFAETEFFEMKKERYVRIEALLNHVMKDGKRLGGKPCLKTLQERGIRSLDTIHKTYLR
                     QINPHIYKVSLSEKLKALKIEILVSHRRKAAKIT"
     gene            complement(21206..21922)
                     /locus_tag="PLF67_00725"
     CDS             complement(21206..21922)
                     /locus_tag="PLF67_00725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOE88566.1"
                     /translation="MCLIAAPLFSVDNPFALPLGTIVIDAGHGGHDPGAIRSFEGSLI
                     VERELTLDIALRLERLLNEAHPSLNVVMTRRDDRFLTLEERGRIAYQTVLPPKTSALL
                     LSIHINSAEHPAAQGFEVFTKLKEKEVPILDSKTPKEHIALFSKHNKKELNILLFEET
                     RKVATVVSSALVKGLPMQVNRGIKELDLWVLNAARMPAVLVEAGFLSNDKELALLLDP
                     QYRQTIALALFHAIEALLST"
     gene            complement(21943..22842)
                     /locus_tag="PLF67_00730"
     CDS             complement(21943..22842)
                     /locus_tag="PLF67_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88567.1"
                     /translation="MTTNYRIYSARDMHTSYGVDQLAIALIDSGSAEELIESIELYQR
                     SMPGVPLFVITQDGRFDFPRLQELYRRISLIVFSGNTYIGEKVNAVASVCNTNYFLLL
                     RTDAVVVTFEYDRLLSLMSRSNHPVVASAIIANAYREIVPCLRTPTPFEKILDPGSDF
                     PSMDPDKPDHTLYPVMGLGFYDRALFQRLRGYDELIHSDFFQLMDLGLRSYLFGYRIT
                     LSPDLLIQFPNRESIIEDRSERTGFERCYTKNLSIRRVKGKNKVRKPPANYDRSSYRG
                     EVRERHQWLQKIDYDGLVTAWQK"
     gene            complement(22832..23956)
                     /gene="proB"
                     /locus_tag="PLF67_00735"
     CDS             complement(22832..23956)
                     /gene="proB"
                     /locus_tag="PLF67_00735"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOE88568.1"
                     /translation="MKRDLPQVKRVVVKVGTNLLSTKAGIDEAYIDTIAAQIAALMEK
                     GYQVLLVSSGAIGMGAKELRLKGPVKQTALRQACASIGNPLLMSSYRRAFKAHNLICS
                     QILLTRLDVNNRRTYVNLRNSVSTLLELGVVPIFNENDVVSIDSLGSAFGDNDRMSAF
                     IASKIDAELLIILTDIDGLYDADPKKDPEANLLSEIDTLDSTILSYASGAGSTYATGG
                     MKTKLLAAKIASFAGCGTIIASGYESDIMVRILDGEQLGTYIHPAKALSQRQRWILNN
                     SFQGAIVIDEGAKNALLRKKSLLPKGVVKVDGTFAEGAIIEVRTPDGSPFARAVSSFS
                     SADLRQVAGHDSSEFTAILGNSHKGVIFRPEDMVFLEHDD"
     gene            complement(23953..25281)
                     /locus_tag="PLF67_00740"
     CDS             complement(23953..25281)
                     /locus_tag="PLF67_00740"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOE88569.1"
                     /translation="MMSHSVKERIKALKESSRVLGVSTNEERIDLLGKVAAGLRRDWP
                     TIRKANEQDVAEAKEQQIGAALIKRLVFDEQKLNEVVLGLEQVAALADPVGKVLQRRL
                     LDDGLILEQISVPIGVIGMIFESRPDALVQIVGLCIKSGNAIILKGGSEAKRTNLALV
                     ESIKQSSRGSSLGDGYVTLLESHGDVKEMLKMNDEIDLLIPRGSNAFVTYVMENTSIP
                     VLGHSDGICHLYIDEQADLKKALPIVIDAKTQYPAACNAIETLLVHASLVDVFLPEVG
                     KALKEANVTIHGDERTQAAIECIPLEEGDYFNEYLSLELNIRVVDSLEEAIGHIETYG
                     SHHTDAIVSENPEAVRRFFTEVDSADVFANCSTRFADGFRFGFGAEVGISTTKIHARG
                     PVGLDGLMSTKFLLAGSGQIVAAYSGKDAKRYLHKNLSVDAPSLVQGGSV"
     gene            complement(25354..26394)
                     /gene="selD"
                     /locus_tag="PLF67_00745"
     CDS             complement(25354..26394)
                     /gene="selD"
                     /locus_tag="PLF67_00745"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOE88570.1"
                     /translation="MKKEVQLTAFSKFAGCGAKVTPAMLDKALCGLVQPYNPNVLSDF
                     NHAEDAGVYKLSDDLAIVQTVDFFPPVCDDPFFFGKIAATNAISDIYAMGGVPITAVS
                     IVSFPEEKLDSSYLTKILEGALDALKESGAALVGGHSIRDEQLTFGLCVVGTVHPKKM
                     WSNNRAQDGDLLLFTKPLGSGILQNAVKAKVAGKGDEERLLKVMSTLNKDAAEILKQF
                     DVHACTDVTGFGLLGHLSEMAIDNPNGFEIVMKDVPLMEGVRLYAEQGFIPGGSYTNR
                     DHRKHLISNLDELDETGQLLLFDPQTSGGLLAALSAGEAHEALKVMRKAGIEAAIIGR
                     VSKEIEGIVVRV"
     gene            complement(26391..27977)
                     /gene="yqeB"
                     /locus_tag="PLF67_00750"
     CDS             complement(26391..27977)
                     /gene="yqeB"
                     /locus_tag="PLF67_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HOE88571.1"
                     /translation="MNIFEQAADLQDRNIPFAFVSITKSVGSTPRSNAHMIVKKDGST
                     IGTVGGGIAEFTVTKEAVAAIAEGKSTHVDVSLAVTDGHACGGTLEFFVDVIASKRRL
                     LLFGGGHVNEQIARLGAGCGFRIEVIETRAEYATGERFPDAGAFHVGETVEEAMKSLE
                     IDRDCAIVIATHGLDKSVLEAVITSDAAYIGMLGSRTKVNTYRRALESERNISIERLD
                     HFYSPVGLDIGSETPHEIAIAVMAEVMMVLHDRSGQSLSRKAENLVVVRGAGDLATGV
                     IVRLAKAGYRVCALEIEQPTTIRRTVAFSEAVYTGEVALETVVCRRAESDQEAKTLLD
                     QGIVALMVDPSASMIERLRPFAVVDAIIAKKNLGTHKGMAPLVIALGPGFEAGVDCDY
                     VIETKRGHDLGKVISRGFAEPNTGIPGKIGGFAEERVLHSASAGTFVGHKKIGDLVKQ
                     GDVIAAVGTDEIIAPIDGVVRGMLHDGIVVPTNFKVADIDPRGIASYCETISDKARAL
                     GGSVLEVIDGMRAKAFRRIS"
     gene            complement(27974..28690)
                     /gene="yqeC"
                     /locus_tag="PLF67_00755"
     CDS             complement(27974..28690)
                     /gene="yqeC"
                     /locus_tag="PLF67_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HOE88572.1"
                     /translation="MTFSLFDELGTHIESLSARRIAVTGSGGKTSIIIGLADQFKEEG
                     VLITTTTHLASPRDMEYPFDEIAIDPNNPPKVEPGRQVLWAAPDSDKLGSVDPELLQE
                     ASKLAGYTLIEADGARRRPFKFHNEGEPVIPPWVDCVIAVVGLSGIGKRLDEETFHRV
                     GEYRAATGSKEAWITLDTLDVLLDHPDGILKGTSAHPVILVYNQSDAARDEVVAEALR
                     LGRTKKRITAVLAGTCRGEV"
     gene            28800..29897
                     /locus_tag="PLF67_00760"
     CDS             28800..29897
                     /locus_tag="PLF67_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88573.1"
                     /translation="MKRLMLIATGLLLAAALIALPASEAAPKTLSYSATDASGRTITL
                     DGKPEKVLVAAKAALMPALALFLFPEAQEMEISLSKTDQGLGDFFPLVWPHINPGGRL
                     DQNASVEELITKQSDLILLKATHYESMGKKLDQVGVKNFTLSLETWDEWQAELIQLGK
                     LLGSEERARHVLDVYEKRIAALKQRVAHLSDDQKPDVLLLQGARSDNAYSFKIAPDSW
                     LQTWVVELVGGNPVWKGSNKAASGWSVVTFEQIAAWDPDLIVTISYNTPASIYQDAIL
                     DSPVWKELAAVRTGKVYASPSDLMNYIQPVASWILGAQWLAKTIHPELFSDLDMDAEI
                     RSFYTDFYGITDMTLLDEFVSRFEKSVVNNR"
     gene            29894..30904
                     /locus_tag="PLF67_00765"
     CDS             29894..30904
                     /locus_tag="PLF67_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE88574.1"
                     /translation="MSEYEKQKRQKMITLTVLLALSVLFGILSLFLGRYPKAGFTSPV
                     LFYTDSVARTILTKIRLPRVVLALASGAMLGASGFTFQLLFSNPLVEPGFLGVSQGAA
                     FGAALMIIFAGASSLSIQLSAMIFGLLGLVFSYYLSTRFRFGGPLLRLVLAGIAVSAF
                     FSSGLGVIKLVAEPTKDLQDITFWMMGGLWNASWGSIIPTLVVIAIALTILHMNRFTL
                     NLLSINEKTSFSLGLNPKRQRVILLVVATVGTTFTISVTGLIGWVGLITPHIARKTFG
                     SEATHALPASMVMGAFFLLLCDSIGRTILPTEIPIGLLTSLFGALIFVLILSGKSQEG
                     KA"
     gene            30901..31656
                     /locus_tag="PLF67_00770"
     CDS             30901..31656
                     /locus_tag="PLF67_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE88575.1"
                     /translation="MTFLSIEKLSYQYPNGINALQSVTLSIDKGSSVAILGSNGAGKS
                     TLLDILLGYKPSTSVSLYGKPLSTYGRKELGRLIALVPQKEQFSFAYTLLDYTLFGRA
                     PYLSPMGIPTSSDNLIAYEALAKVGLGGFENRSITALSGGEQQLLIIARAIAQESEIL
                     LLDEPTSSLDPANRKRILTLLKQLHSEGKTLIFTTHDANFAYELSSDIALLKGGTLLG
                     YGKKEEVVKSELLTTLYDTQLTVTEIDGRTIIY"
     gene            complement(31650..33392)
                     /gene="ade"
                     /locus_tag="PLF67_00775"
     CDS             complement(31650..33392)
                     /gene="ade"
                     /locus_tag="PLF67_00775"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOE88576.1"
                     /translation="MDKKKLQNLIAASARRKSADLCITNAHILDVFNKEWFEADLIIS
                     EGHIAGFAPPGKGKAKKIVDGGGRFLIPGLIDSHVHIESSHTTPEEFSNLVVPHGTTT
                     VIADPHEICNVAGLDGLHYMLEASKESALQMFFVVPSCVPAVSFEHAGAVITAEDIRS
                     VMDHERIVGLGEMMDYPGVIAGKDLVIDKIMVAKEAEKVIDGHSPAVYDADLDAYSAS
                     GIKTDHECETPAELRDRVRRGMYVHLREGSACNNVIGLLGGVDSKNSRRCCFCTDDRQ
                     PVSILEEGHINNNLRIAVHEGLDPIEAICMATINSGECYNLTDRGAIVPGRRADFCLA
                     DDLVEFRMHQVYVDGVLVAQEGKMLKPSKAKKDQRVASKMNIKDFSVERLDLRLKHNR
                     VRVIDIVPGGVVTEQGEAFVKLEKGVWVHDPNQDVVKLAVIERHKGLGTVGVGLIRGF
                     GLRKGAIASTVGHDSHNLIVAGDNDADMAKAVEHLIETGGGMTVIQNGKVLSTLEMPV
                     AGLISYEKGEVVAEKLQHLHDMAIEKLSVKPGIDAFTTLSFMALPVIPTYKLTDMGLF
                     DVGTFSFVDLELDQ"
     gene            33759..>34138
                     /locus_tag="PLF67_00780"
     CDS             33759..>34138
                     /locus_tag="PLF67_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOE88577.1"
                     /translation="MDKFFKITERKSTVKTEVIAGITTFLTMAYILVVNPGILSATGM
                     SFEKVFSATAISSAIATLIMALMANLPFALAPGMGLNAFFAFSVVLGMGYSWQFALTA
                     VFVEGIIFIILTFVNIREAIVNSIP"
     assembly_gap    34139..34238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34239..35212
                     /locus_tag="PLF67_00785"
     CDS             <34239..35212
                     /locus_tag="PLF67_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOE88578.1"
                     /translation="FIILTFVNIREAIVNSIPANLKKAIGVGIGLFIAFIGLQNAGII
                     VNNDATLVALNPGWFKGGPGLAMLGLLITGVLLAFKVRGALLIGIIITTIIGIPFGIT
                     QYAGGKYVPPAPYFFQFEFSKAIFSLDFVVVMFTFLFVDMFDTVGTLIGCATKADMIQ
                     KDGSIPNVKEALFADAVGTTVGAILGTSTVTTFVESSAGVVEGGRTGLTALTTAILFL
                     LSLFLVPLFSSIPSVATAPALIIVGVFMITPVVDIDWDDMTEAIPAFLTLLFMIVAYS
                     IAEGIMFGIISYVLLKLFSKKGKEIGGMTWVVFALFVAKIFFGALGS"
     gene            35308..36282
                     /locus_tag="PLF67_00790"
     CDS             35308..36282
                     /locus_tag="PLF67_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE88579.1"
                     /translation="MTPLDSTKEYFVTGVAGFIGFHLASSLLKQGCKVIGLDNLNSYY
                     DVRLKRERLKLLGCERFTFYHADLTDKKKLETIFQRHPITHVVNLAAQAGVRYSIDHP
                     DAYIDSNIQGFLNVLEAIRTHPVEHLVYASSSSVYGKNTKIPYSIHDRTDEPVSLYAA
                     TKKANELFAHTYQHLYSIKTTGVRFFTVYGPWGRPDMAYYSFTKKILNNETIDVYNNG
                     DMWRDFTYIDDVINALEHIIPRVPTDPVIYNIGNNNPIRLLRFIEALEEVIGKKANKN
                     FLPMQDGDVYQTYADISDLQRDFGITINTPIEEGLASFVAWYRSYYNV"
     gene            36285..37457
                     /locus_tag="PLF67_00795"
     CDS             36285..37457
                     /locus_tag="PLF67_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOE88580.1"
                     /translation="MYDVIVVGGGVAGLFLAANLPDRKVLILDHAKSVGKKLLITGGG
                     RANITNDLSAKEMLAHFPTKAMRNFLAPSLLSLTPSDLMNWFENRGVELIVRADGHVF
                     PSSLDARTIRNALLSHIHADIRTACTVAAIINEEESFTIHSSEGSFVTRSVCLATGGM
                     SYPSTGSDGSGYALAKALGHTIVPPKPALTPIVTCEQTAHLSGISLSNVSITHRQFTA
                     TGDLLFTRDGLSGPVILNLSRAVSKGDPVTIAVLDSSLLEENLLSFPQKRVSTIIRTL
                     GVPASLAREICERSHLEPDRNAGTLRKEERKLLCKTAGALTYKVNTTKGFKQAMATSG
                     GVELSEVNRKTMESRLTPGLFFCGEVLDYDGESGGFNIQAACSTAYLAARTIGKRT"
     gene            complement(37532..38731)
                     /gene="ygeW"
                     /locus_tag="PLF67_00800"
     CDS             complement(37532..38731)
                     /gene="ygeW"
                     /locus_tag="PLF67_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOE88581.1"
                     /translation="MKDKTAIKTMIEKLKTLKSEGMYLDDFFLTWDKTDDELAAVFQV
                     AEILRAMREANISPKVFSSGLGISNFRDNSTRTRFSFASACNLLGLEVQDLNEEQSQI
                     AHGETVRETANMISFMADVIGIRDDMYIGKGHTYMKEVASAVQEGFDQGVLEQRPTLV
                     NLQCDIDHPTQTMADMLHVINHFGGVENLKGKKVAMTWAYSPSYGKPLSVPQGVIGLF
                     TRFGMDVVLAHPEGYNVMSEVEEVARKNAESNGGSYKRVDTMAEAFKDADIVYPKSWA
                     PFAVMEERTEIVEHNDQAGLKELEQRCLLNNAKFKDWTCTEELMATTKDGKALYMHCL
                     PADITGVSCKEGEVEASVFDRYIVDLYKEASYKPYVIAAMIFLARFKDPAAKLEQILE
                     KGKQRIK"
     gene            complement(38841..41717)
                     /locus_tag="PLF67_00805"
     CDS             complement(38841..41717)
                     /locus_tag="PLF67_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HOE88582.1"
                     /translation="MTIQFTVNGKRHRVDVDGSETLRAMLVRLGYTNVRDSDDGDGFA
                     GSDTIIFNDLPVYANLMNAVQADGGEILTADSMGNSRNLNPVQRAMIDAGVVQSAYNA
                     PQAALLLTWLLKHEPNPTRERIKEVLSGIFIRDAGYEHYYLAVQLARELIEFGKYKTP
                     ISPTFRDHLTYVGKPKGKVDGPTLVAGEAAFVEDRLQAGYYSMAILRSPHAMAYITEI
                     DTATAEALEGVVAVITHKNVPDVYYMQAGQGNPEPSPYDRKLMNRKVRHVGDRVAAVV
                     AETIEIAREALKLIKVTYEVLKPVFTVEEAMAEGASLVHNSVVEYVVGAPEGLDEYNK
                     GADPRDGKVIYQFPLHGDIRRNIASAAHGEIGDVEKGFKEADLVIERTYQTNQIQCTP
                     LEPHVCYAKIDGGRLVLHASTQVPYHVRRIVARVCEIPEHKVRVIKERVGGGYGSKQD
                     ILVEDLVGYATWLTGKPVYYRNTREEEFIANSTRHPMRITMKLGAKKDGTLTGVYMDV
                     RANTGPYGNHCLTVPMNACSKTLPLLKCDNMKFDVYTYYTNIPPTGAYQGYGAPKGTY
                     AMMTALAELADGLGIDYYDLVDKNKIESGYMLEILRGLGEGREGKVVPVGSCGLGEAL
                     AKGARMIDWGKKEKGPGGDWKIGKGFAIIQQGSGLPGLDHSNAWAKLLPDGNIQLFSG
                     GADIGTGLDTIHAKIAAEGLFVTLDQVNVTSGDTDSCPFDTGAYASSGTYFSGNATYY
                     AIEDLKRNILDEAAHQLGEQAEDLALRAPGEVYSTKTGASVSYATLGYNALQGDGRGQ
                     VMGKGSFVTEHNSIPYGAHFVQVAVNTRTGQIKVEKYYALQDAGTPINPEMALCQMYG
                     AALKSIGHTLYEDMTIDEKGYIQNVRLGDYGVPMITEQPEDFKAILIDVDDEVGPYGA
                     KSISEISTNGAAPAIAVAIHDAVGVWMRSWPFTPEKLLREMGKL"
     gene            complement(41714..42532)
                     /locus_tag="PLF67_00810"
     CDS             complement(41714..42532)
                     /locus_tag="PLF67_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOE88583.1"
                     /translation="MKKFLVAQSKEEALSLKRLHAKSVFYAGGTEINRLESAVQAEVA
                     IDLSKLGLNRIEEKKDEIIIGSMATLQTIATSSVVPAWLKEAALFCGSFTRRNMATIG
                     GNFALMLGRSYVGPVLLASRAILLIATINEKGKYAEDEVPACEYHEHFASYQGALILG
                     FKIRKSDRFVGTLRFAVSSQNRASVTVGFGAEKDRSKVRVYAAVYTVGVLRLKETESR
                     IEKGEITTEEEIAKSVAAEVTVKSDYTGSGEYKRYIAGEGVAMLYGEFLKGGQR"
     gene            complement(42532..43920)
                     /locus_tag="PLF67_00815"
     CDS             complement(42532..43920)
                     /locus_tag="PLF67_00815"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOE88584.1"
                     /translation="MSSSLLLKNIYTLQTSFDKEALHGADLLIIDNKVGRIDPAGKIS
                     SKGVDRVIDCSKHVVLPGLVNTHHHFYQTLTRNHPAVQNAKLFDWLTFLYDVWKYLDE
                     EGIYYSSLLAMAELMRTGCTLTTDHHYVYPHHVQGDPMAIQFEAAETLGMRFSPTRGS
                     MSLSKKDGGLPPDTVVQTEEQILRHSEDSIRRFHDSSADAMRKVALAPCSPFSVTKEL
                     MKETAALARKYGARLHTHLCETADENDFCLQMYGMRPVELMQECDLIGEDVYYAHGIY
                     FNDEELKIIADTGSHIAHCPSSNMRLGSGICRVAEMLPMGINIGIAVDGSASNDSSDM
                     LAEVRQALLLQRVRYGSDALTAKEAFTMGTINGAKMLNFTNVGKLEEGWAADAAIWDL
                     STAAYAGAQSDPVAALLFCGDSHYTDYTIINGKVVVDRGTLVGFDEALLAEKANAISK
                     RMLDRAARKEAL"
     gene            complement(44136..45359)
                     /locus_tag="PLF67_00820"
     CDS             complement(44136..45359)
                     /locus_tag="PLF67_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOE88585.1"
                     /translation="MNMHEKIKARAKELRSYTAENLSKMVKTKSYSSQEEDVARLIVT
                     LLEEAGFDEVYIDGLGSVIGRVGNGPKKLAFDAHIDTVEVGNPDLWNFDPFSGEIKDG
                     IVYGRGASDQKGGAASMITAGRILKELGYGGEYTVYFTFTVMEEDCDGMCWKYLIEEE
                     GLKPDLVVSTEPTSCRLYRGHRGRMEIRVILSGISCHGSAPERGVSAAYKAARAALAM
                     EQLNKDLQPDEDGFLGKGTITVSVMDVKGPSQCAVADYAMLYLDRRLTWGEDADMAIA
                     QVREYISKATGDAPDSFIVEMPNYEKIGWTKKEYSQELYFPTWKLDEDHFLVQSGIDA
                     YKTLFGKKPVVDKWTFSTNLVATTGRHKIPAIGFGPGDENQAHAPNEINRVDDLEICA
                     AFYAMLPYSLEARGK"
     gene            complement(45401..46723)
                     /gene="ssnA"
                     /locus_tag="PLF67_00825"
     CDS             complement(45401..46723)
                     /gene="ssnA"
                     /locus_tag="PLF67_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOE88586.1"
                     /translation="MADLVIKNTRIMQTEPPFQVLEGVDIVIDGNTITKVGKDAAKGV
                     AAKKVIDGTDKTVMPGNVCSHHHYYSGLSRGMLIQAGPQRDFIQVLKEWWWRLDRGLD
                     EEACYYSSLICSLDAIMAGTTTAIDHHASPSYIAGSLDTIAKGMEKVGIRGSTCYEIT
                     DRNGGMAEVEAGVEESRRFIERSKKSSLVKAMIGGHAPFTIPNEGLKLMGDLVEKTKV
                     GIHLHVAEDRYDVAHSHHRYGMDIVDRLDEYGLLTDNALLVHGLYLNEDEIRKINERG
                     VFFAHNPRSNMNNHVGYCSYIQDVKNLLIGTDGCGGNMFEELKIGFFKHKDEGLSWWP
                     PQYVEALNRGYRLVEKYFDGSFGRVEAGMVADLVITDYHNPTPLVQENAASHFIWGMS
                     SNCVESVIVDGKVVMENRAFAHLDVDEIYREAAKVAKRVWKEVDTIAP"
     gene            complement(46727..>48435)
                     /locus_tag="PLF67_00830"
     CDS             complement(46727..>48435)
                     /locus_tag="PLF67_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE88587.1"
                     /translation="LSVIYEKNALPAITSHICDHQCQLHCTRMDYEGAVKIRDMKRIA
                     VERGFADYLQLWEGATEKTEYRAAVIGAGPGGLSAAYFLARAGFDTTVYEREESAGGV
                     VRHVIPGFRLPVEAIESDVEFIKAHGVEFHFGVDTKKLTVETLKKQGFTHIFYAIGSE
                     IDNELTLVGDRTRVITSLDFLGQFRKDPSKCTLGKHVVIVGGGNTAMDGARAALRIPG
                     VEHVSVIYRRTRAEMPADHEEFENARAEGAEFIYLANPEGFEGNMLTVRKMVLGEKDA
                     SGRRRPIPSDETFTIRADTMITAIGEKVDGEIFSWYGVPLNEKGWAITDAKSKASAID
                     GVYVIGDAQSGPSTVVRCIASARDAVEDAIDRILEAEGVEIDEDEEHIHDEEDQPIEE
                     LVAEEDEYFGELELRKSRILFSKLFGEQGFARREADRCMECSYICNKCVDVCPNRANV
                     PIDLRNTGLFDNPFQILHLDAFCNECGNCETFCPYDGGPYKKKFTLFSRRDDFENSTN
                     SGFLVEDGTILIRVDGRLYPCELDKDGVLVGEEEGLSDEVAAIVEEVFASYSYLLGEV
                     EE"
     assembly_gap    48436..48535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<48536..50049)
                     /locus_tag="PLF67_00835"
     CDS             complement(<48536..50049)
                     /locus_tag="PLF67_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607491.1"
                     /note="with YgfM and YgfN forms a selenate reductase,
                     which seems to catalyze the reduction of selenate to
                     selenite; YgfK mutants are unable to reduce selenate;
                     involved in purine salvage process; seems to act as an
                     oxidoreductase, but sequence similarity suggests it is a
                     dihydrothymine dehydrogenase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOE88588.1"
                     /translation="MSDIMRPVPFVELLHRIVNEYRNHGSIFSISEDLFYHDKGEKQL
                     GIFGQTCSTALGPAAGPHTQLAQNIVASYLVGGRFIELKTVQILDTLEVDKPCIDARD
                     EGYNVEWSTEFTLPKAADEYIKAWYILHVLDVTLNDREFGKPSFIFNMSVGYNLEGIK
                     TPRMQAFIDTMIDAGKDERFARYRNELETLLKEGLFEGTPWEGKEERLLRTIDKISPN
                     LTPSVTLSTMHGCPPAEIEAICSYMLTEKRLNTFVKLNPTLLGYENVRRILDNLGYDY
                     ITLKQESFDNDLQYPDAVKMLHRLVDLAKEKGLGFGVKLTNTLGSVNDQEQLPGEEMY
                     MSGRALLPLSTNVGLLLSKEFDGKLPISYSGGATAVNVKALFECGIHPITLATDMLKP
                     GGYTRLKQMVEILESSDGWKRETIDVPALEKLVADIQDGLYTATTKEFRGTDSIKIHE
                     QLPLVDCYVAPCVNACAIHQDIPEYVQLVGEGRYGEALSVIYEKNALPAITSHI"
     gene            50654..51112
                     /locus_tag="PLF67_00840"
     CDS             50654..51112
                     /locus_tag="PLF67_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88589.1"
                     /translation="MKEYLIGFAIGLIIALAVFIYYVVKRANDQRAHAKEVARLKNML
                     SDRMDIESEGLRTLKEENAELKKQNENLRITLNTLSQKPGRKEVNRLQVYQLAVDRLT
                     INSPGFGPAWQAALKESEEEFQKNLTGATAFIKRLIPVKTEAAVLPETID"
     gene            51131..51214
                     /locus_tag="PLF67_00845"
     tRNA            51131..51214
                     /locus_tag="PLF67_00845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51165..51167,aa:Leu,seq:taa)
     gene            complement(51335..51916)
                     /locus_tag="PLF67_00850"
     CDS             complement(51335..51916)
                     /locus_tag="PLF67_00850"
                     /inference="COORDINATES: protein motif:HMM:NF018749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HOE88590.1"
                     /translation="MIIDKYPFLWSNEPKHVFEDGSVTLTTSGSTDFWQRTHYGFRAD
                     NAHALLTRIGDEFTLTAHCTFEAKNQYDQGGLYVRIDEENWIKISIEAENDEISRLGS
                     VCTNLGYSDWATVDIPASINSMHYRVSSKGDDLLVEYSYDGNDWTQMRICHIHAHKDH
                     YLAGMYACSPKEAGGAVYTFDRISIEKSIWKNE"
     gene            51976..52599
                     /locus_tag="PLF67_00855"
     CDS             51976..52599
                     /locus_tag="PLF67_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOE88591.1"
                     /translation="MHTLFIYGSDALDHLKRNDSRLGRAIDRIGMIHRTIEPDFFTAI
                     TRSIIGQQVSTKAQTTIFERLLDLVGILTPEAVLRLSLEELKSAGMSFRKAEYIQGIA
                     HKIVSGKLNPTLFASMSDSEVIKELVSLKGVGVWTAEMILIFCLQRPDVVSYGDLGIH
                     RGMKLLYGLEEISKERFEEIRARYTPYGSVASLYLWAIAQGVLSPLD"
     gene            52670..54631
                     /locus_tag="PLF67_00860"
     CDS             52670..54631
                     /locus_tag="PLF67_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HOE88592.1"
                     /translation="MIEEQGLTKEEIKARKLLEEKEADSKLRSYPGVFGHAVTVLFII
                     WSIFQLYATTIGKIDAMRLRTWHLLFLLMFTFLLFPTYKTEPLKRKAPPVWDWLLLGL
                     TFFSVGYLLLYYTTVAKRGGYLNKTDLVVAFILLAVIFEAGRRACRSLMYLGLIFLLY
                     NFLGRFIPGDLGHVGFSLKRVLSHMVWGSQGVFGVGIGVSATYIFLFVLFGAYLKYSG
                     FSQFINDVSLTLVGRSAGGPAKVAVIASALMGMINGSAIANVATTGTITIPMMKKTGY
                     KKEFAAAVEAVASTGGQFTPPIMGAVGFVMAEFMGVSYTKVLVAAAIPAFLYYLSLLF
                     AVHFEAKRLGLSGLSKENIPDALEVFKKQGHLLIPLVVLLVLLGIGYTPLFAAVISIF
                     ATVGASWLRKETRMDWRTIVQATVEGARSSITVGMSCVIIGVIIGTVSLTGLGLSFGF
                     VILRIVGEGQLFLGGLMVMIMSTVLGMGVPGVAAYVIVSAVSVPVLIQTGAIPMAAHM
                     FCLIYACLSNITPPVAMSSYVASGIADSNQTKTSLIAVKLGLTGFILPFFFLDNPILL
                     IGSTPDVPILLTIRAVITSSIGVVALSAGLQGYLLRNLTIIERGLLVISGLLFIATSL
                     STDLIATGILITLLLIQYFQTHRMKERIA"
     gene            54628..55596
                     /locus_tag="PLF67_00865"
     CDS             54628..55596
                     /locus_tag="PLF67_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOE88593.1"
                     /translation="MKKLITILLMIALVVPVFAAGGGEKAPAKTTINFPTAATTGAVY
                     PLGAAISNMWNNKVPTVRAAAQASAGGIENLNLIADGEAQVGVAVTSIMYESFTGTGT
                     FEGRANPNLRVMIGLYYNPNQVVVAKNSNINSLNDLAGKSFASGAPGSTTEVETAIHL
                     KESGINYPDALKVQYIGFTEAIDLMRNKQIDGAWIMAGIPNAAVTEMLSTAGGKLLPL
                     DPALIDSLHAKLPWYAAYTIPANTYPGQDEDVLTSAIKLTICTDARVSDDVIYELTKA
                     FWENIDELKATQAPLKNTFLEETVRDLAGLPIHDGAARYYRERGLL"
     gene            55777..56136
                     /locus_tag="PLF67_00870"
     CDS             55777..56136
                     /locus_tag="PLF67_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88594.1"
                     /translation="MSSFIKDYQRGNRIYEWDGRYLKAYQDGQKMIEWDGQYVKSYSN
                     GRRLFVFDGRYFKRYSDGQKLYEWNGRYITAYHDGRKVIEWDGRYIKSYTNGRKLLEI
                     DGLIPLAIIIGVATGMM"
     gene            56245..57249
                     /locus_tag="PLF67_00875"
     CDS             56245..57249
                     /locus_tag="PLF67_00875"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HOE88595.1"
                     /translation="MEQAFIDLHLSLELQRKVKHLSNRIGDDSQHRLLIDELRSAIVD
                     RDQNRVGLLLNQGLTGFGVLHPLVAIAQVWETIGEVFHALGLDDETRLATLNDINLWI
                     AHHAEANNGKQGLSRVFWVSRLLTARIVQFGRLQFEERVFPYPATVYQDFGLEFIALS
                     NGGLTLDRDGYPTKDANKITRTTVGRELIDLSSGSIGETRIDRSLLTKVLDEQSRVLF
                     VHIPASGPLDPHLVEEALRKAEAFFGRGWVYVCTSWLLDPALEKVVDSESNILSFNRR
                     FLKLPVFFEYPQLFERVFFCNDEASVLSADATTSLQKNVQSALRRGVVFRTMGGFFLA
                     "
     gene            57277..58071
                     /locus_tag="PLF67_00880"
     CDS             57277..58071
                     /locus_tag="PLF67_00880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE88596.1"
                     /translation="METPLHGLVDSHFHLLAIREKGIDPSPLLATMKELGYRGIDIGL
                     GGNDLAERIGLVGGYKEIYFATGLGPWAAAWEDWRVKEALQLLVSDLARYPVLAVGEI
                     GLDNYWKYGTTERQEELFLAQADLGKPIIIHNREADEQFIRLLEEHSFPFGGIFHCFS
                     GSEELADLALEKGFYLSFAGSLTYKGSEPLIALLKRIPKERLLLETDSPYLAPVPKRG
                     RKNIPLYIEYLYRFVANKLELPIETLIRLVSSNFQRFVDEAKVDRP"
     gene            58125..59117
                     /locus_tag="PLF67_00885"
     CDS             58125..59117
                     /locus_tag="PLF67_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOE88597.1"
                     /translation="MISYKDLGLVNSKEMFAKAIKGGYAVPAYNFNNLEQLQAIIQAC
                     VETKSPVIMQVSKGARDYANINLLRNMARGAGEYARELGYDIPIVLHLDHGDSFETCK
                     DCIDNGFSSVMIDGSHLPYEENVALTKKVVEYAHKHDVTVEGELGVLAGIEDEVSHEK
                     STYTKPEQVVDFVSRTGVDSLAISIGTSHGANKFNPAHCTVNEEGILIPPPLRFDILE
                     EIERRLPGFPIVLHGSSSVPQEYVKIINKYGGKLKDTIGIPEEQLRRAAKSAVCKVNI
                     DSDGRLAMTATIREILHNNPGEFDPRKYLGPARDELKKMYIHKNTNVLGSANQA"
     gene            59269..59817
                     /gene="infC"
                     /locus_tag="PLF67_00890"
     CDS             59269..59817
                     /gene="infC"
                     /locus_tag="PLF67_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOE88598.1"
                     /translation="MATRDLRVNRQIRAREVLVIDEDGNQKGIMSSFDAIELAEQAGL
                     DLVEVSPNANPPVCKILDYGKFRYEQEKRLRDQKRNQVITKMKEIRMQPKIEQHDLNT
                     KSKFIGDFLTEGNKVKVSIRFRGRELAHTELGKVVLDRILAQLTDNGVGYVVDRPAMM
                     EGRMMSMILSPAKTSGPKKEPQ"
     gene            59895..60092
                     /gene="rpmI"
                     /locus_tag="PLF67_00895"
     CDS             59895..60092
                     /gene="rpmI"
                     /locus_tag="PLF67_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004790647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOE88599.1"
                     /translation="MPKMKTRKSAAKRFKVTAGGKVLYKKQGLRHILTKKSSKRKAHL
                     RSTGVLSDVEARRVKSMLPYE"
     gene            60104..60463
                     /gene="rplT"
                     /locus_tag="PLF67_00900"
     CDS             60104..60463
                     /gene="rplT"
                     /locus_tag="PLF67_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOE88600.1"
                     /translation="MPRAIDGTKHKDRRKKILKLAKGHRGRRSTNYRVAKDSVEKAGQ
                     HAYVGRKLRKRDFRRLWIVRINAAVQAEGLNYSQFMHGMKLANIEVNRKALSNMAIED
                     KAAFKTLVDQVKVALAK"
     gene            60480..60839
                     /locus_tag="PLF67_00905"
     CDS             60480..60839
                     /locus_tag="PLF67_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88601.1"
                     /translation="MSVIDTVRLLELRTKKAAGLIALLRREKEELQQRFDLVLAHNRE
                     LEGYIEAYTTSEKLVEQSIATAMATLDTIDGLDEIQVFDDASLELEAADTFTGADGGL
                     SSEDVDWDALLDDLPEL"
     gene            61163..62347
                     /locus_tag="PLF67_00910"
     CDS             61163..62347
                     /locus_tag="PLF67_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE88602.1"
                     /translation="MHEALNHVIFISLPPSMERKIGSFTIDPAIRLPVALADDHTAID
                     ERSISIERIVSGMLKVLAYDIDHQHAPYYREFVLAAQPDAVKELTIAAIAQQQKGNLT
                     FAEELFLTVCHLSPQSATYINLATLYGHRATEDSSKGETYDFYQQKALNTLVEGLSVL
                     GDDEALLKEIGFFHLYQGNVEIARDYLVRYVDLAGESKEKRHTMKILKDIETKLAGEQ
                     RLMQAYDAIHMNREEEALSLLNRYIEAYPEVWNAYFLKGWALRRLERFQEAQDALIQA
                     LELESHSSDIYNELALCALEAGNEELAKSYLGTALDLDEENITFMSNLAYLHLRSGEL
                     EEARHLLEQARSLDGEDPLIQRLMEDYTHQSGDTLGDVIIEEYLENEEIADKLKHGAK
                     EI"
     gene            62344..62763
                     /gene="tsaE"
                     /locus_tag="PLF67_00915"
     CDS             62344..62763
                     /gene="tsaE"
                     /locus_tag="PLF67_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOE88603.1"
                     /translation="MIVRTTGEKETIRVGYALGKTLAKGAVVSLRGSLGVGKTVFARG
                     IAQSLGIREAIVSPTFTLIQEYEGTLPLYHMDLYRIDSVEEFELIGGEEYLYGEGITL
                     IEWSEKIQELLPEETISVTITLAEDGSREIRIEGSML"
     gene            62760..63434
                     /gene="tsaB"
                     /locus_tag="PLF67_00920"
     CDS             62760..63434
                     /gene="tsaB"
                     /locus_tag="PLF67_00920"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOE88604.1"
                     /translation="MIILGCDTSTKTANLFLGEYEEDQLSWYETTSVVWNNTHSEQLL
                     DRLLLMCSDHSIELSELDLLCASSGPGSFTGLRIALATMKGLALGLNKPLVSVPTLDL
                     FQGIARFIGGACLAVIDAKKQRFYTALFVDGIKIGESSDLSPDEIASLIAPHPSITLL
                     GSDGTLLAKRLADPKVAVFEAHRQNLGVELADRARKHYLETGADPLDQGPTYIRKSDA
                     ELALKE"
     gene            63439..64383
                     /gene="trxB"
                     /locus_tag="PLF67_00925"
     CDS             63439..64383
                     /gene="trxB"
                     /locus_tag="PLF67_00925"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOE88605.1"
                     /translation="MVEQEILVLGSGVAGMAAGQYAARAGRTVTIMESIAPGGQTMYI
                     DLIENYPGIEVPVSGAEIALKFQSQAETFGAKIVYETATSIRKEGDDFLVETASGTTY
                     KAKAVIYATGAKHRHLEIEGEEAYAGKGVSYCGTCDGPFFRGKRILVVGGGDTAITDA
                     IYLSKLTDHITIAHRKDRFRAQDNLVKQIERNPHVEFLMEHTLQEIKGDGNKVTSVIL
                     KDLKQDREYEREFDAVFIFVGIIPETNLLKGSYDVLTEDGWVITNERMETSLEGLFAA
                     GDVRDTVFRQLITAASDGAVAAHCASSYIDELEGRAYT"
     gene            64432..65889
                     /locus_tag="PLF67_00930"
     CDS             64432..65889
                     /locus_tag="PLF67_00930"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOE88606.1"
                     /translation="MKICMVSSESVPFSKSGGLADVVGALSKALASLGEEVLVVLPHY
                     GFMTDELIPTDIEHTVSLLGSEERVTYAKLSEPGVEYYFVQHPFFTESKGIYGPTSSS
                     SYEHNHLRYTLQAKASLELLKILGWKPDIIHAHDWTAGFLPYLVKRDSSGFFEKTKTV
                     FSIHNLAYQGEFSRLDLLETDIEVDPRFFVGSGPKKRANMLKTGLEFADTLSTVSPTY
                     AKEIQTPEYGCGLEGLLQRRKDDLVGIINGIDIDEWNPENDRLLDYSFSIDDLSNKAR
                     LKRQIQEEFNLEVNAGIPLFSMISRLVSQKGFVELLEGEVSALEELLTTEEIQVVVLG
                     TGDWELEKKFTELGARFKNLSVNILFSNRAAHLLEAGSDFFLMPSRYEPCGLNQFYSL
                     RYGTLPVARRTGGLADSITDLDEDPQRGTGILFDSMTGRGILEAVRRALTWWKKGRTV
                     IEPIRKRAMAYDSSWTRSARSYQELYRKTQGGFTK"
     gene            65886..67184
                     /locus_tag="PLF67_00935"
     CDS             65886..67184
                     /locus_tag="PLF67_00935"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOE88607.1"
                     /translation="MNRRKPNVIAIVLGGGKGTRLYPLTMDRAKPAVPFGGKYRLVDI
                     PISNCINSGIKKIYILTQFNSASLHNHISNTYIFDTFSKGFVEILAAEQTIKTETWYE
                     GTADAVRKNLRHFRDQEADYFMILSGDQLYRMDLMDMLNRHIDSKAEFTIAAKPISRA
                     QATGLGIIGCDDKGIITSFIEKPAPDVDITSYKVGPKFMKESLGVSVTRENEYLASMG
                     IYIFNSKTMEEVLDNKKTDFGKEIIPQEISKRRAAAYLFDGFWEDIGTVKAFYETNLD
                     LASINPMFNFYDEEMPIYTHRRHLPATKINFCNISNSLTSEGSIITNAYIVNSIIGVR
                     TLIESGASLDGVYCMGASRYETDEEKEQNAKKGIPNIGIGRGTIIRKAIIDQNARIGD
                     GCRIGIDDIPRQEGDFPMYSIHDGIIVINKNAVIKNGTVM"
     gene            67187..68773
                     /locus_tag="PLF67_00940"
     CDS             67187..68773
                     /locus_tag="PLF67_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOE88608.1"
                     /translation="MDERKRQIERGETILGIELGSTRIKAVLIDSHNEPIAQGGHTWE
                     NQLVDGVWTYSLEAVEEGLQASFKSLNDDIKAQYNVVLKRTKALAISAMMHGYLAFDN
                     EDNLLVPFRTWRNTMTEQAAEDLSKLFDYPVPERWSISHLYQAILNKEEHVPRIAKIT
                     TLAGYVHYRLTGKHLLGIGDASGMFPVDPKTKGYDRTMVKQFASLHTHPWRLEELLPA
                     PLSAGEEAGVLTKEGALFLDPTGTLEAGIPLAPPEGDAGTGMVATNSVAKRTGNVSAG
                     TSVFAMIVLEKELSRSYNKFIDLVTTPDGSLVAMSHANNCTGEYDAWIALFDEVLHAV
                     GHSVPKAKLYDDLLGKALEGDYDGGGLLAYNYLSGETMTELYEGRPLFVRKAESSFTL
                     ANFMRTQLYTALGVLRIGMDILFEQEGVSIDSITGHGGFFKTALVGQKMMASALHTPV
                     ATLKSAGEGGAWGIALLASYLVNKKGRTLAEFLDQDVFAGAERTIVEATGAEIEGFNK
                     FMERYKEGLGIERAAVEFLR"
     gene            complement(68770..69153)
                     /locus_tag="PLF67_00945"
     CDS             complement(68770..69153)
                     /locus_tag="PLF67_00945"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOE88609.1"
                     /translation="MEERNTTAGIVRQDGKLLIAKRVEGGPVGGLWEFVGGKCRHCET
                     PEEALVREFEEELGISVVVGKLLATHTFENNHVRYHLGAYEVKRVDSKPITLSVHTEI
                     AWVSFSELDAYTFPPSDQAIISQLR"
     gene            complement(69162..69572)
                     /locus_tag="PLF67_00950"
     CDS             complement(69162..69572)
                     /locus_tag="PLF67_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88610.1"
                     /translation="MIQLYLLSVAYLVIGSGFLLSDTHGIKMSILFSLRFTFRNNRAV
                     RLGLIIGGFLLTIGLALFPIDPGPRIIGDLLPLINIFILSIWYLTESLQKSEADELFT
                     SVVGRYRERSRERMGYVTLVIALIHFLLPHFVLL"
     gene            complement(69569..71053)
                     /locus_tag="PLF67_00955"
     CDS             complement(69569..71053)
                     /locus_tag="PLF67_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HOE88611.1"
                     /translation="MKIVTSSEELKRVDSAAKKVLPSLVLMESAALQCWFALSRLIER
                     SSSLLFLIGGGNNGADGLAIARHAYHAGWRKITVLHTATRYTDEHAFQQTLLKEYPIR
                     QLWLGDIDLDDLPSRDWIIDAITGLGLNGPLNEKLHDLISWVNHSDASVFSVDIPSGL
                     GDRCAVSNLCISSDHTVALGSFTQAHYHPLARSRCGNLRIANPSFPLALLDSLNVIAT
                     VDDEPPAIPALNRSAYKNTRGSIAIFGSSEKYTGAARLAASAAFTARSGLVTLFCDPE
                     IHEICASNTLSVMVRSYRGEPVEGFDAILAGPGWGEGREEILKVLLSMSTPLVIDADG
                     IRSLARLIASKEYSVESRNAPLILTPHLGEMRVIGEAVLGRPCFLSSESPESFFSQLK
                     AIAQTLDATIVLKSSLIHIVDPDGTITILEGLNPSLGVAGSGDLLGGMITAFLGQGFS
                     AHDSSLIAGKILFKSALTANERIGYYDSETLLPYIGLAIKEAER"
     gene            71192..71530
                     /locus_tag="PLF67_00960"
     CDS             71192..71530
                     /locus_tag="PLF67_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88612.1"
                     /translation="MTKKIEFKYIFEKDYNPKYVNGAFGGMSPTGEMVVNFYLERMPL
                     PYSDFHDVKEGQVGDLTGREPEDYDSTIVRYIQNGIVLSLEHAKAIHNWLGLHIAEAE
                     RIEAQKKLPR"
     gene            71543..72115
                     /locus_tag="PLF67_00965"
     CDS             71543..72115
                     /locus_tag="PLF67_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88613.1"
                     /translation="MHSEFTSSSTASNIVTAPEAKTFSRRGLLEDTNSLSSHYRLADI
                     RVVDFLKNASTSCFSHSLNNSQIKRYFENTGTFAMQMNRVEKHLQSFKDLKTGWNGYD
                     APPIPEAVIRIAGDILRHIRYVVPEIFPTGRESVQFEFDHNDHSLEVEVFKDAIHVDL
                     FNDTDLIKEWSLDLDGLEGINECIAELYGY"
     gene            72105..>72429
                     /locus_tag="PLF67_00970"
     CDS             72105..>72429
                     /locus_tag="PLF67_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88614.1"
                     /translation="MDTKKVGEEEVLYRGITTNPTMWKSSENRPSSAAFKQTHSLSVD
                     REGGRSFVEIKENLIKNISSNLKAVAYFSAKDCFYNHMTVRPDPVINNPYHALIDDEG
                     GVGVGK"
BASE COUNT        16237 a        20391 c        19209 g        16392 t
ORIGIN      
        1 ccctctttca aagcattctt tgactgttat tggttttttg cactcagttc aaggggaatc
       61 cggatcaggt gttcatcgct tgcccgatag aaggtggcga tgaacccgat gattgagaca
      121 agatcgcttt ttgtccgttc ggccaggctc tcggcgattg gtccaagctc atccttgtgg
      181 gcttgaaact tcacctttac cagctcgtgg ctcgaaagcg cctcatcaag ggcgcggaca
      241 acctgttcgc tgagcccgcc cttgccgacc atcacgatcg gcttgagcga atgggcctga
      301 gctttcagaa atgcgcgaac cgaggaatgc atcgcatcag tccttgtact tctgggcgac
      361 gacgtggcgc ttgacctttt tcgtcgaggt catctccagc ggctcgttca ctaccgtgac
      421 ccgggtgatc ttcttgtacg attggagcga cttgttcact tcaccgacga tctgattcag
      481 ccgcttttca atctcggccg ctgattgttc gccgtgagtt ttcttcatct cgtcggaata
      541 cttttccgaa ggatagatga gggctcggat tccttcgctc tttttctgct tgtcgacgat
      601 gtagccgaga acacagatcg tgtcaatctc atcatagagc tggaagtggt cctcaatctc
      661 ttcggggaag acgtttttgc cgccttcggt gacgatcagg tttttcgccc gtccggtcag
      721 gtagagatag ccgttggcat cctgataccc gacatcgccg gtattgagcc agccatcctt
      781 cagcacctcg tcggtcgcct ccgggttctt gtagtacccc tgcatcacca tcggtccctt
      841 gatatggatg agaccgttcc cgtcgctgtc cggatcgacg atccgcacct cgacttgcgg
      901 gaggttcttt ccgacggagg tctcgatata cgcttcgacc ggattgaggt gggtaatcgg
      961 gctggtttcc gtcagaccgt acccctgaac aaaatcgatt cccagctgat tgaacagctt
     1021 gaaggtggat gccggaagcg gtccgccgcc gctgatgcag atccggttgt gttccagaga
     1081 gagcttcttc aagaggaagc cgaacatctt ctttccgatg ttcaccttga agaccttctt
     1141 caggacgccg cttacaccca tcaggaagcg gattacgccg tagaggacgg ctcccttctc
     1201 cttcacaccg ttcatcagag cagcgatcat cttgttgaaa agcatcggaa cggcgaggaa
     1261 catcgtgacc tttccttccc gcatctcctt caatacctgg ctgatgacca attttttccc
     1321 gaagatgatc gaagcgccga cgctgatcgc ctcaatgaac accgccatca tcgtgtagga
     1381 gtggtgcagc ggcaggatcg cgtagaatac gtcggtggga tagatccaca tgttcccctg
     1441 ggcgaggtag caatcgctga ccaggttctc atggctgagc atgacgcctt tgggggttcc
     1501 ggtggttccg ctggtaaaaa gaatagccgc ggtctcttcg acggtcgcct tgtatgcttt
     1561 ccttcggcgg tcccccttct gttccatgat catcggaaac tcttcactcg gctcaaggct
     1621 gaggcggtgg gtgagaccga gggtgtcttt ttcgtcgaac cctttgagcc gctcgctgtc
     1681 ggcgatgaag atcttcactc cggcaaacgc catcagtttg atcatgtctt catcggagag
     1741 gcttgcatca aggggaacca cggtgccgcc ggcatagagg attccaagat agctcatcgc
     1801 ccattggggg ctgttctttc cggtgagggc gaccttcgtc cctttggtta ccccttcaac
     1861 cacgagatag gaagcgatcg tttgaatatg ctcatacgcc tcggcgtagg tatatgcctc
     1921 tttcttcggg gtgaatgcta tgaaactttc attgtcaggg tatcgtttga cggtaatgtc
     1981 aaacatctcg acgacggtcg gccaggttcc ggtaaactcc ttccccctgt actcatcaag
     2041 aaaatcccac ggttttgcgt gatgcttctt tttggacatg aattcccctc ctctgtttgg
     2101 gttctaacac tataaaagaa aaacccgctt tttggcaaca gattaacacc ttatcacatt
     2161 gcgtaatagt gtaatgaatg agattcgttg accaaaaacc ggcaagtgtc tacagtatcg
     2221 gtcatggaat tgaaatgctt tgccgacgtg gttgcgtact tggaagggtt tttgaacctc
     2281 gagaaaaaga ctcagcacta cacgacccgc gaataccggc tcgatcggat gatcgccctc
     2341 ctggaccacg tgggcaatcc ccaagaatcc tttcaatcga tccaccttgc cggaagcaaa
     2401 ggaaagggtt cgaccgcctc gttcatcgca agcggtttga ccgcaatggg gtataaaacc
     2461 gggctgtata tgtcccccca cctcgtcgac taccgggagc gtttcacctt aagcggcacg
     2521 tacttcagcg atgaggcgtt ggtgcgtgcc ggcaaccttc tgaaaacgtc ggtcggggat
     2581 ttcagcttca gtgacgaatg gggcgagacc acccccacga ccttcgagct gttgacgacc
     2641 tatgcatacc tcctgttcaa agaggagaag tgcgagtggg cggtcgttga aaccggcctt
     2701 ggcggccggc tcgatgcgac gaacaccctt caaagcagcg caagcgtcct ctgtccgatc
     2761 gaactggaac acaccagaat cctcggctcg acgatcgggg agatcgcctc tgaaaaggcg
     2821 aagatcatca aagccggtca accggtgttc atcgggctcg tccccgaaga agcgaaagag
     2881 gtgttcctca aggagtgccg caagcagcgc gccaagccct ttttgctgga agagctcctt
     2941 gccggatatg agacccgcac gaccttggaa ggagaacggg tgacgcttgc gtggagcgat
     3001 ggaacggaag atcgactcct gctttcgatg aagggttcga tccaagccca gaacgtcgcc
     3061 cttgcccttc tgacgctgag gacccttggc ctctttggcg ggaggacgat ccatgccctc
     3121 gaggcgaaca cggtgcccgg ccgctttcag caggtcgccc cctccctcta catcgacgga
     3181 gcgcataccc ccgcttccct gagcgcattg acccgatcct ttgatgagct gtatccgtca
     3241 aaagatgcga acgtggtcat ttatggcgct cttgaagaca aggaccacct ccacatggcg
     3301 ccaatcatcc ttcaacactt caacaagatc atcatctccc ggccgggaac cttcaagaaa
     3361 agcgatctgc aaaagctttt ttccatcttt gaagccttga aagcggaatc ccgctccacc
     3421 gcctcgatct tcctgataga agacaatagc caagcactcg agaaagcgta tatgctgaag
     3481 ggagaagacg gagcgattct ggtgtgtgga tcattctacc ttggaggtgg aatcgcggag
     3541 gccctcaaga gaagatgagc ttgaattggc gtgaaatcga actcattgta agcgagctcc
     3601 ccttgaaagg ctcaaggctt caaggggtaa cccaacacga tttcaacgcg atcagcttcc
     3661 acttttttca ccccgttcac cgccagtgga ccctctatac cgaggtggga accccctcct
     3721 cgcggctcca caagcttacc cgagctcggg caaaactgcc caaaacgcag cgcttcgtcc
     3781 aattcctcaa agcgaacctt gtcaactgtc tggtctcgga ggttgcccag accccgggtg
     3841 aacggttcgt cgatctgacg tgcacccgcg gcgaagatac gatgcatctg cttttgcggt
     3901 tctacagcgg agcgggggcg aacatcattg tgaccgacga ctcgtacacg attctggatc
     3961 ttctcctccg acgtccccgc cggggcgaag caagcggtct gtccttcgcc ttgcccgaac
     4021 cgaaagaaat cgatggtgca tttcctattc gcaggaggac gaaggaatcc ttcaacgagc
     4081 agatcgaaga ggagtatgcc gccgagcgga acatccggac acttgagtcg ctgtatgaac
     4141 gggcaacgct tctctacgag aaaaaagccc gctcccttga gcacactctc gcctccctga
     4201 acacccgaat cgatcaaacc cgggacttcg aacgatacaa gcatctcgga gacctcctca
     4261 gttccaatca acacctcatc cgggagggtc aaacctccct gtccgtccat gattgggaga
     4321 ccgatagaga agtagaaatt cctctcgatc ccactcgatc acctcgagat gccatccagc
     4381 tctactacga gagctaccaa cgctcgaaag gccggtatga aaacgccctc ggcgagctga
     4441 aacgggtaca agaggagctc cgtgcccatc aggcgatctt tgaacgggcg cttgatcgga
     4501 gtcttcccca agaagaagcg cttgctttgt tgacgagtct ggtcgaagag tcggaaaaag
     4561 aaccgattgt tcgcccgagc gtgggtctgg tctttcaaag cggggcatac acgctgctcg
     4621 tcggtcgaaa cgccaaagaa aacgatgaac tgcttcgccg gcacaccaga ggcaacgatt
     4681 ggtgggtcca cacccgcgac gttcccggcg gctatgtctt catcaaatac atcaaggata
     4741 aaccgccgcc gctggaggtc ctccttgatg cggcgtatct cgccattctc tattcgaaag
     4801 caaaaaaagc ggggaaagcg gaactctact acacccaagt caaatacctg cgccgggcga
     4861 aaggagcgaa gatgggcacc gttttgccta cccaagagcg aaatctttct gttacactgg
     4921 acgaagcgcg cttgaatgtc cttctggcgc aaggagaagt gtagtgacga cctatcacca
     4981 tacgattttt tatcctgaag aacgggaacc gctggatgaa gctacatata agcgaagcga
     5041 ggcgggaacc ctcaaaagcc tcccttccgc cctcctgctc ccccacgccg cctattcgat
     5101 gatcggggag gctcttcatc agacgtttgc ccatgcatca agcttgaaac cggaactcgt
     5161 cgtctttctc ggtccccttc atcaaccggt ccttgaccgg gacaagcccg cgttcctctt
     5221 tgcccccgcc gcagatacct ttgagcaagc gggacgaacc tatcagtttg acgaagcgat
     5281 gattggtgag ttgaccttgg ggttcccgat tcgtcgggca gcctcctatt atgaagagga
     5341 gcctgccttt gaattgactc ttcccttcat cgacagctac cttgcgccca atgcgatcct
     5401 tcccttgatc ggaaaggttg aagattccaa tcagatcgct caactgattc ttgcgttgaa
     5461 aacaatcgtc caacgccagc caaaggtgtt gttcgttctt tcggcaaacg cgagcgagct
     5521 gctgccccaa cgcgaagcca ccgatgaagc gaatcacttc attgaggacc tgattcatga
     5581 acatcaatcg ttgggaattc ggagctcctg caactggggc atgatcgaag caatgaatcg
     5641 aagcgcactg gtcaaaggaa gatggaagat cgcttcggcg tttctgaaag gaaccgacca
     5701 tggcagaatt acggacacca aaaacgaatc gggactgatg acgtggcata tcggggcgta
     5761 caaaggagtt tagggagatg aagctctatc aaccgttcaa tacgacctat aatgatgagg
     5821 aaataaccgc aatccccttt gcgagcgcag cggtcgagct gccggcgttc cacctgcaag
     5881 gggaacgagc cgcctcccat ctctacaagg acggcaagct tacggcgtgg tactggcagg
     5941 gaatcgccct gatcgagaac caacgctccg tctactttcc ccgccaaaac ctcttctcct
     6001 ttaccgaact ggcgaccgtt cgccgaagca aagcgctctc gtgggtccgg cgcctggcca
     6061 aggcgctcac cctcaccacc ggcagttttc tcgatctctc cagcggaatt cttcccctgt
     6121 ggcggatcta cggaggcgag gacggctcga tcctcatcct cagccaggat cttggcgacc
     6181 tcttcgccgc catggcgaac gatgaaccga aattcttcaa catctcggcg tgggttcatc
     6241 ataacattca cccggcattc accctctgcg atcaactgac gcagctgctc tactatgcaa
     6301 ttacgggaac ccctccattt ttacgcaaag aaacccggga agaccgctat cgccatcttc
     6361 cgcttgcata cagctttgaa gaggtttctc ttcagacccg gcttttcatc gatgcaagcc
     6421 tgagcctttc cctgacgaaa caacgggaca gcacggggaa caaggaaccg caaaaagccc
     6481 tcacggcgtt tctggatgcc accgaatcga tcgagtggga tgcggaaaac cgaacggagg
     6541 tgcctccccc ttccgcttgg cagaacaccc cgaagattca ggagtttctt gcgagccaag
     6601 ccaaacgagc caaacggatc gtcttctggc ggatgaaggg atggctcatc atcacgatcg
     6661 ccgtcagcgt catccttgtt tcgtggttta caatcgatcg agtcagcgaa gcgctgaaac
     6721 ctccctatac tcagttcatg gatccccccg cgatcatcac tgaatactat caaggactga
     6781 acgctcttga tctttcccac atggatgcgg ctttggcgaa gaaggtgaaa aacccttgga
     6841 cgatggaggt caccaacctg ttcgtcacgc gacagacccg caaagcatat gaagggttct
     6901 cgcccacggt cgatcccaat gaatggtttg ccggcggcca aaaacccttg attgaaggct
     6961 ctttcctcta tggaacgacc gatgtgacgg taacccggct cgatgaaaga acatttactg
     7021 ctcaagggac tctctatacc ccgtatccct atgacgaaga ggaggccgat caggaacatc
     7081 ggcaaagcgg gtcgtatatg tacaccctca cccagacctt caccctcgac gtgggaaaga
     7141 aagggtggta cgagatcacc tccatctctt cgccccggat cactcgcatc ggctttcttg
     7201 aaatcgagtc tatccctcgc ctcgaacaaa cgcctccacc cgctcgatga ttgaatcgac
     7261 atcgtcggca ccaaaccact cggcagcggc aaagcttctg aaaaacgtca tctgccgctt
     7321 cgtgtacagc ttcgtattgc gctcgatccg gcgaagaacg gattgaacgg aatattcgcc
     7381 gctttcggaa agttcaaaaa attcccggta tccgatcccc gccatcgagg cccatgtgct
     7441 ttgggctcct tgcctaagga gcgagcgaat ctcttcgacg aggccatcgg tttccatctg
     7501 tttgatccgc cgtttaagcc gatccagcag ctcctgccgc tcccgttcca agccgatgat
     7561 caagatggga ttggagatcc tcttctcgct tggaagatcg aacgaagaga ggggtttgcc
     7621 gctttgctca tacacttcaa gggcccggga aattcgatat gtgtcgtttg gatgaattcg
     7681 atcggcactg accggatcga cttccgcaag gcgcttgtac ccccaagaca gacctttttc
     7741 ttcaatgagg gcgccaacac gggcacgcac atcctcgttg cttgagggtg cttcactcag
     7801 accgtagagg aagtgtttta cgtagaacgc cgttcccccg ctgatgatcg gaatcttgtt
     7861 ttggctgtga atcaacgcta ccgcttcatc ggcggcttcg atgaaatcgc cgacggaaaa
     7921 ggattgatcc atctcccgaa tgtcgatgag gtggtgttcg atcgcttcac gctccgctcc
     7981 ggtcgccttc gccgacccga tatccaagcc cttgtagacc tgaatggaat cggcgttgat
     8041 gacactgtag cgtgccgagt caaaccgact gagaatggcg gtttttccta ccgcagtcgg
     8101 tccaaagaga atgataatcg gtttgatagt ctcagaggtc gtcgggctct tcgaagagga
     8161 tctccccggc aagggctcgg ctgaggactt cagtgacaat tttgccatta ttctcctcga
     8221 tctctttgcc cccaagtcct gggttgctga gaatatttgc ttccttgatc gctacgcagg
     8281 taatccggta gatattgtct tcgtagtcaa tcaggctatc aaggggaatg ctcaccatgg
     8341 tactccttca tcacagttcg gcgctttacg caccggcgta catcctaaag ggtttggaaa
     8401 aaaaagtaca gtccccctag cccttctctc ggctccgaac ctcatgaaca atgatatgcg
     8461 ctacttcagc cgcacttttt cttgtcgtgt cgatgaggat atccgctacc gtcgaatcgg
     8521 tgttgtcgat atcgtagagc cgtttatacc gagcggtatc cttggcgtct cgggcggttg
     8581 tttcggcgat cttttcctcg agggtgcctc cctcgcgctt atggattcgc cgcgcccgct
     8641 cctcgaccgt cgcatcaaga tagatcttga gatccgcctc tttgagcatc cagatcgcca
     8701 accggctggc gaggatgcaa tctttttggg cgagggccat ttcaacttgc cgtcggtcga
     8761 gctcaaagtc gatcgaatca tccttctccg ccaaggcggc aaggtcccag aactccatgt
     8821 gccgctcttc ggcgaggttt cggaaggtga agttgatcag aggataccca agctccttgc
     8881 tgaccaatga agacaccgtg gtattcccac atccgctttt tccgctgatt gcgattcgca
     8941 cacccaatct cctattcgat attgcgcact tgttcccgaa tattctcgag gctgtttttc
     9001 atcttcacca cctcgaggtt cagctcgaca agctggcttt ttgatccgat cgtattgatc
     9061 tcccgattca tctcctggca aaggaagtcg aggcgcttgc cgaccggctc ctttgtctga
     9121 agcagggaat ggtactcctt caggtgaatc ccgaggcggc tgatctcctc ggcggcggag
     9181 tagcggacca gcatcaccgc gacctcctga agaatccggt tctcatcaaa ctgcttatca
     9241 cccagaagct cgtcaaagcg cttcagcagg ttctgcttga tatgctcttc aagccggtcg
     9301 gcaccggcct ctatgaccgc caagctcgcg ccgatctcct ttccaagctc ggtgagatgg
     9361 agggcggtcg ccgccccttc ccgctccttc gctagagcaa actggttcaa cgcctcatca
     9421 agggcggcaa agagatcttc ccggtagagc tcgccgtccc gctcggaaat gcttacaagc
     9481 accccctcct ggctgagata atcgctcaag gccggcttga gggccttgcc gctgattgcg
     9541 ccgatccggg caaatgcctc gctgaggcgc tcgacgacct ccttgtcgac gaccacttgg
     9601 agatcgcttt tgagggtttt gaccttcacg aagacctcga cgcgcccccg ggcggctttt
     9661 tctgaaacca tgttggaaat ctccaactca tagccgctta aaaacggagg aaggttgcag
     9721 atgatttcaa ggtatcgatt gttgtacgat ttgatttcaa cgctcagtga aaagactgaa
     9781 tgcgcttttt ccgcatatcc atagcctgtc atcgatttca cggaacactc cttaggaatc
     9841 ggctgttgag ggcgcgattg ttgccagcac ccatcgtaat acacaaccac ccttctttca
     9901 accgcatcag ggcttgggcg accgcctcct cgtcgtccgc cgcatacgtg caatcggtat
     9961 ctgccttgag cagggcgtat aacgccaagg gatcggtttc ctcatctttt ctggctgaag
    10021 cgtaggtcgg ttggaggatg agctgatcgg cgcccctcag tgcggcagcg aactcctcaa
    10081 gaagggcgtg ggttcgggtg gcggtatggg gcatgaagag cacgaggatc ttcctgccgg
    10141 ggaagcgggt gcgcatctcc ctcagggaaa cgatgatttc gctcggatgg tgggcataat
    10201 cgtcgaaaaa gaggattccg gcgcgttcac ccatgtattc aagccgcccg acgaccccgc
    10261 cgaaggtcgc aaggcgcata gcgagcccct tcatccgaac gggatcgatt ctcttttccg
    10321 gaatcatcga agcaagcaga agcgccccga gatgatcggt caccagttcc ggcgttccaa
    10381 cggtgaggcg ggtggagatc cctccgattc gatagtgttg cttcccctct tcgacgatcg
    10441 gataatcgct ttccggatcg aagccgaagc tgagcaccag cagatcggtt cgcctcgctt
    10501 tggcatactc cccgattgcc cttgagcctg tttggtcgtt attgtagatg aagaagcccc
    10561 cctgctcgat cccatccacg aagcgcctga aggaatcatg cacgtccgca aggtcgccaa
    10621 agtaatcggg atgatcgtat tccaccgaga ggagtaccgc cccacttgtt cggtagagca
    10681 gaaagtggtc ctgatactca cacccttcga agaggaagaa acgagacttc ttccctgaag
    10741 aattgcttct tttgagcaat gttgaaccgt agatcgccca atatcctccc tcttcgccca
    10801 gcagatggtc ggccattgcg cacgtcgtcg tttttccgtg ggttccgatc accaaatagg
    10861 tcggccgaag cgtggacagc agcgctaaaa attcggggta tgaatagagg ggaatgcctc
    10921 gttcctttgc gtggatgagg atgtctgtcg aagggggata ggcggccgag tgaatgaccg
    10981 catcccacgt tccattgaga ttggagcgat cgaagccgag atcggcagcg atccccgctt
    11041 ccgtcaggct tcggccggtg aaaaaatcct cttgggtgtc acagccgccg atccggcagc
    11101 ctccgtttgc caacaacagg gcaaggcgag ccattccgct gcctttgatt ccgacaaggt
    11161 agatccttcg aaacggaaga ttcatgaatg cagaatacca aaaaaaagac cgcccgataa
    11221 ggggacggtc ctttcgctca agatgatctc ttccttaggc ggaagctttc ttcttgaggt
    11281 tggcgttgca gggcttgcag actttcgtct tcgccccttc caaggttgct tcatagagaa
    11341 cctttacgcc ggttcgatta catttcgggc aggtgccgcg gccattctta tatttatcac
    11401 ggattccact gcctctgtgt gcttttgaca tattcccttc tcctatccgg ttctaacgtg
    11461 ctaacgcttg actatatcca ccaaagcccg ggagtgtcaa ggttgacgaa cccctagagg
    11521 aacaggcgca aacccacgag ggccttggcg ggccgggggg atggcttgtt cacataccac
    11581 gcggtcggaa actcatagcc gatgagaatc ttcatggtgt tcagcgtgat cgccagcgtt
    11641 tgattctgga aaccctgtgc gaacttcaac ccgagccaat cggtcgcttc ccccttttgt
    11701 tcatgatagc cgagaatcag ggagatgtcg taggtcggca gcagattgag ggttaccgag
    11761 gtgcccatcc tgagctcccg ttcttcgctg gaccccatat tcgctatatc caccgccgct
    11821 cgtaccgtaa ataggtggag ctcattgcgc tcattgtatt tcggagtttc aaccgccatt
    11881 ccccacccga gggacttcaa taacgaatca aaggagatgt tcagcgtttc gctggtttcg
    11941 gaagaggcga atttcgtcga tgtgaccgcc atgctgatcc aggagtaatc aagcaggaag
    12001 gagacgccga aggagatgct tccgatctgg tcgaccggct catagcggct gaagttggtc
    12061 gcaacgaagt agtcgctcca gatgagctcg ggtcggaagg tcatctcgct gcgttcggac
    12121 agcgcctcga tctgggcggt gaacccgatg tagaacggct ggcggccgaa ggcccaatcg
    12181 aattggaaga ggcccgtccg ctttccggtg tatgtatact ccttgatccg agtcaccgaa
    12241 gagacgttca tgatcgagaa cgccatccgt ccggcgatga atgaaacgcc cagattgacg
    12301 ctcggctctt caaaatccca tgattcggcg gaatcttcaa aacctgagga aaggatgaaa
    12361 ctccgagtcg tccgatcgct gcttgccaac aacgcgggat tccccaagaa ggaatcactg
    12421 tcgctgccaa gggcgaatcg cgcaccgccg aacgcccaca ttgtctgcgt cgtgctcttg
    12481 gcggtgaggg aaagagaaac gagaagaaat atgactgtga tcgtcaccaa gcgcttcacg
    12541 aaaagcctcc tccgtcagca tatcaaaaag ttttacgcta tgctatcgcc tgccaagctg
    12601 gtatgatggc catatgaaag cccttggaga actcctccgc tcagcccata ttccggtgac
    12661 atccacacag cagtctttta tcgtcacctc cttgtgcacc cattcgaacg cgtgcaggga
    12721 gggttcgatc ttcttcggtt tccgaggtca aaacagtgac gggtcattgt acgccaagga
    12781 ggctttacag aacggcgcca tcttagcgat cattgatgaa gaagcttcgg tcgaagacca
    12841 tcaattcatc atccgggtca aacatcccca caagaccttc gcattgatga acgccgcctt
    12901 ccatgacaac cctcaggcgg agttgaagct catcgcggtc tcgggaacgg acggcaaatc
    12961 ctccaccgcc gatttcctcc accagctgct ctctttcgaa ggaataccgg ccgggctctt
    13021 ttcaacgacg tcgatcgatg acggggcggg aaagatcccc tccccctacc ggatctccac
    13081 cccggaagcg gatctcctgt actcgttctt ggatcgctgt cgagccaatc gaatggaata
    13141 cgtgatcatt gaagcgacca gccacgccct cagcgaggag tacagccgac tctacggcct
    13201 cacctttgag atcggcatca tcaccactct cagcggagat catatcgagt ttcacggttc
    13261 aagagaagca tatctcgaag cgaagctctc cctgcttgga atgattcgcc cccggggcca
    13321 tttcatcacc tccgttgaaa accgggaact cgaccgatgt ttgaaaacat tggattcctc
    13381 catccacccc catatcctcg gtcttgatca cccgtataca atccaaacct gtgatttgca
    13441 ttccgtccgg ggaacatttg aaggacggcc gttcacggtt catcttggac ttgaggtgtt
    13501 tgcgacgaac gctctgctcg ccctgctcgc ctcaagtgtc atcactgcta agaatccggt
    13561 tgagttgctg cctcatctat cgaacgttcg tcccctcaaa ggccggatgc aagcagttcc
    13621 caacgacaag ggtgttcttg tttttattga ctatgcccat acgatcggag cctatgagca
    13681 cctctttgct tttatcaagc aggttcgagg aacaagccgg gtgatcaccg tgatgggggc
    13741 cgccggcggt cgggacaaga aaaagcgttc cgttctcggc ggcatcgctt cgaaagaaag
    13801 tgatatcctg attctgaccg aggaggatcc ggtcttcgag gagttgagcg ctatcaacgg
    13861 gatgatcaga aacgggatcg cttcaaagag ctgcagggtg ctggagatcg acgatcgaaa
    13921 agaggcgatc ggagaagcga ttcgccttgc ccaggcaggt gacatcgtcc tcctgctcgg
    13981 caaaggtcat gaacagtcga ttcagcgcag gaaccggaca atcccctggg atgaagaaca
    14041 agcggtgttg gaggtgattc gatgaacgta gccctgatct atggcggcag atccgctgaa
    14101 catgaggtgt cgatcaacag cgccagaacg atcgaacgga tcctgaagcg caatcatcac
    14161 accctctatc cgattgcgat cgatcgaaac ggctgctggt tcctgcaaac acgggttcaa
    14221 aacacgattg atgaagagat tcctctttgg atcgttcccg gaagcgggat atacgctcaa
    14281 agcggcatgc ttccgatcga tgtaggcttt cccaccactc acggccgggg cggcgaggat
    14341 ggaaacctcc aagggctttg cacccttgcc aaaatccctt tggcaggctg cgacaccgtc
    14401 tcctcggcaa tcgggatgca caaggcgttg gcccaatcgg tcttcgatcg ggccggcatc
    14461 cccacgattc cgacgatcat cgtcaccgaa gcgatgccgg tcaccgactc gctcctggag
    14521 gagcttcgac aaagccttgg ttcgagtctc ttcgtaaagc cggagaacgc cggatcgtcg
    14581 gtggggatca ccgttctcat cgaagccgat ctgccaagcc ttgcccgggc tcttcgcctg
    14641 gccctctcct acagtgagcg ggcgttggtt cagccctatt accctgaagt aatcgaagcg
    14701 gaatgcgccg tctttgaaga tcccgaacaa ggaatcatcg cttgcggacc ggggatgatc
    14761 aaaaacccga agaaagaaac gttcctctcc tacagccaga agtataccct tcaaggcgga
    14821 gcgtaccttg agcttccctc cgggttgagt gatgaagtcg aagaggagat ccgctctttg
    14881 gcggttcggg cgtttgaggc gatcaagggc aatggatatg cccgggttga cttcttcctt
    14941 atcgacgaga gaccgatcct caacgagatc aacacgctgc ccgggttgac ggcaaccagt
    15001 cattggccga agcttcttca atatggcggg tattcgattg agcgcgtcgt cgacctcctt
    15061 ctggagcagg cgatctcaga tttcgatcga agatggaatc tgaggacgag ccatgattga
    15121 gaccgtcatc ttccacgtcg acatcgatgc gttcttcgcc gcggtcgaga ttctcgacaa
    15181 ccccgacttg gcgggactac ccctgatcgt cggcggcttt tccaagcgcg gcgtcgtgac
    15241 gacggcaagc tatgaagcgc gaaagtacgg agtccactcg gcgatgccga tgattcaggc
    15301 tctccgcctc tgcccccatg ccacggtggt tccaactcgc agaacccgct acagtgaagt
    15361 ctccgcccgg gtgatgggga ttcttcggga gttttcttcc gatgtccacc agatctcgat
    15421 cgacgaggcg ttccttgaca tgagcggaac cgaacgcctc tggggacggc cccgggaggc
    15481 cgccctgaag ttgaaggagc gggtacacga ggaaaccggc atgacgatca gcgtggggat
    15541 cggtcccaac cggctgatcg ccaagatggc cagcgactac aacaagccgg atggactgtg
    15601 caaggtttcc gaatcgaaga aggagctctt catcgacgcg gtcggactga gcaagctctg
    15661 gggggttgga aaggcgacgc aggaggagct gaagcggcat cggatcacca cgacgggcga
    15721 gcttcgagcg attccgatcg aacgtatgaa gaagctcttc ggcaactcga tgggctcctt
    15781 cctctatcac atcgttcggg ggatcgatcc gggaatcttc agcgaagcga aaagccggtc
    15841 gatctcaacc gaacacacct tccttgagga tgtcggcgac cgtgaggtat tggaggaaac
    15901 gcttctttcg atgagccatg aagtgatgtt ccgctcgctg gacgaacgga agatcgcccg
    15961 gacggtcggg gtgaagatcc ggctttccga cttctcgacc tccaccgttc aaaccactcc
    16021 cgccgaagcg atcttcagcg ccgagcaggt ctatgcgatc gcaaaggagc tcctcgcccg
    16081 gaaatggcga tcgggaatga aggtccgcct gatcggcgtc gggctgtacc agctgtacga
    16141 cggcgcttcc ccgatccaag gcgagctctt tgaaagtgaa cacgaaaaga agcggaagct
    16201 ggaagaagtc gttctcgccc tccagaagga gggtcatgta cttatcaagg cggccaacct
    16261 aggcaaacac cccctcggcg atgaggatca gcagggctaa ggcgatccga tagtacccaa
    16321 agccgatgaa cgtatgggtc ttcaggaacg agcgcaggcg tttcatgaca aacagggaga
    16381 tgatgaacgc tgttccgccc ccggtaagca tgatcatcca ctcattggcg gaatagctca
    16441 tgccgagctt caacattttc aggagggtcg ccccggcgat cacgggaatc gccatgaaaa
    16501 aggagaagtt ggcggaaagc ggccgggaaa gaccgatgat cagtccgccc aacacggtgg
    16561 aaccgctgcg ggacgtcccc ggaatgaggg caagaacctg aaacagtcca agcaggaacg
    16621 catgcctgta tgtcaacgac tcgatcgaat cggtgactct gaagctcgtc ctccttgcat
    16681 gcctcttctc cagaatgatg tagagcaatc cgtagaacgc cagggctgaa gcgatcacca
    16741 tcgggccttg aagacgggcg acgatcatat catcaaacag aactcccatg acaccggcgg
    16801 gaatcgtggc gacgatgatc ttcccccaca ggttcaaact cgctaattgc ttcgtcttat
    16861 cccttgtaaa cgggctcaga tccttgaaat agagcacccc caccgcaagg atggaaaaga
    16921 actggattcc gacgaagaag aactctttgg cgcttgccga aagagcaagt ggaagatacg
    16981 gctccgcaag cagcaggtgg gcggtcgagc tgatcggcag ccattcggtg attccctgga
    17041 cgattccaag gagaatcgat gagatgatat cggtcattga cactccttgt ggcagtctag
    17101 ttgaatccct tgaggacggc aagccgtctt gtcgacactc cgttcaaatg atacagtcgc
    17161 cctatgggat tgaacattgt tttggttgaa ccggaaatac cacagaatac cgggaacatc
    17221 tcccggacct gtgcggcgac gcacaccagt ctgcatttgg ttcatccctt ggggttcacg
    17281 atcacggaga aacacgtgaa acgggcaggc ttggattact ggaaatacct tgagctggtc
    17341 gagtatcccg atatcgccgc tttttttgac caacacgaaa aaaaagagtt atactacgtt
    17401 acaacgaagg cggaacgaac ctatgccgac atcgcctatg gtgatgagtg tttctttctc
    17461 ttcggcaagg aaacactcgg gctggacgag gagctcctgg cgaagaaccg atcgaggtgc
    17521 atccgcctgc cgatggttcg tgaagcgcgg agcctgaacc tctcgaatgc cgtagcggta
    17581 atcgtctatg agtattaccg gcagaaaggg tttgaaacgt tagtgaaagc gggagcgctt
    17641 caccaccgtc aatgggagtc gcaagcatga gaaaagtcgg aatgatcggc tgtggcgtga
    17701 tgggcagcgc tatcgcccgc accctccaag atgaaggatt cgtgtatgat atcgatcccg
    17761 agcgcgcccg tgctctggcc gacggaacaa ggtttcaggt atccgattcg ctggagacgg
    17821 tggttcagga atgcaatctt ctcatcttgg cggtcaagcc gcaaaccctc tcaagcatct
    17881 atcccgtcct caggcaggca ggatcgaaag agaaacgctg gatcagcatc gctgcgggag
    17941 tctccacttc gacgcttgcc gaaaatctcg gcaccaccga agtcgttcgg tatatgccga
    18001 acatcgccgc ccgatacaag caatccgtta ctgccgtcgc cgctcacccc gaggcgggca
    18061 gagagctgct ggaggaggcg aagacgatcg ctctcggttt cgggcgtgtc catgttcttg
    18121 atgaacgact gtttgacgga ttcaccgcga tcagcggttc ggcggtcgcc ttcatcatca
    18181 agtttctgga ttccctcgcc cagggcggag cggacgaagg actcgcctat cccctcggtc
    18241 tgacgatagc cgccgagacc gccgagagcg ccgtcaccct gattgaagaa agcggattgc
    18301 agccccggga tctgatcaat cgcgtttcca gcgcaggggg aacgacggtt gaaggcatca
    18361 aagccctgaa tgacggaaac ctgcaggcga ccgtcatgga tgcggtcaga aaaaccgccc
    18421 aacgatcgag agagcttgaa gaagcagcag caaaccatac agagaggaaa tgaggatacc
    18481 atgattgacc tacgggaact gaaaagccgc tatgacgaaa ttatcacgaa tatcaagaac
    18541 cgctcaatga agatcgacct tgatgcgatc atcgccttgc aggatcagcg aagcagcctg
    18601 cttcaggagg ttgagtccct tcgctatgag cgcaacgaaa acgctcaaaa gatgaaaggc
    18661 aagctcgatc aggaaacccg cgcccgcctg atcgaagagg gaaagaatct gaaggcgaag
    18721 atcgccgaaa cggaagaacg cttcaaccgg gttgaacagg aataccttct tctcggccgg
    18781 cagattccca actacgccca cccttccgcc cccgtgggca aagaagatac cgacaacctc
    18841 atcctcaaac gggtcggaac tcctccgacc ttcgccttca aaccgctgga tcacgtcgaa
    18901 ctcggcgaga agcttgatct catcgacttc gaggcgggga cgaaggtgac cggaccgaag
    18961 ttctacttcc tcaagaatga agcggtgatc cttcagatgg ctcttgagcg ctatgctttg
    19021 gacattctca tcaaacacgg ttttgctcct tacatcaccc ccgacatcgc caaggaagag
    19081 atcctggaag ggatcggatt caatccgcgg ggaccggagt ccaacatcta taccttggaa
    19141 ggcacgggaa cggcgttggt cggcaccgcc gagatcacct tgggcggtta tcacgccgag
    19201 gagattctcg acatcaatcg cctcccgatc aagatggccg gtctctcgca ctgcttccgc
    19261 cgcgaggcgg gttcggccgg ccaattctcc aaaggcctct accgggttca ccagttctcg
    19321 aaggtcgaga tgttcatcta ctgtcttccc gaagaatcgg atgcgattca cgagcagctt
    19381 cttgcgatcg aagaggagat cttctccggc ctgggtctcg cctatcaggt ggtggatacc
    19441 tgtaccgggg atctcggtgc cccggcgtac cggaaatttg acatcgaggc atggatgccc
    19501 ggccgcggcg agaacggcga atacggggaa gtcacctcga caagcaactg caccgactat
    19561 caaagccgct ccctgcgggt tcgctacaag gatgccgatg ggcaaaacca atatgttcac
    19621 accttgaacg gaactgcggt ggctaccagc cgggcgattg tcgccatcct ggaaaacttc
    19681 caggaggagg acggctcgat ccgaatcccg gcggttctcg tcccgtacac cggttttgac
    19741 cgaattggag gttaacggat gtttcatcgc tcagcgctca gtaccgattt ctatgaactc
    19801 acgatggctc aaggatattt tctggagcgg aacaatccaa atgtcgtctt tgagatgttt
    19861 tatcgaacca acccgtttgg aggcggatac gtcctctttg ccggcttgca cgatctgatc
    19921 gacaagcttg aagcctttcg tttcagcccg gaagatatcg catacctttc aggtctgggc
    19981 atcttcacaa aagagttcct ggagtatctc tccaagtacc gcttcaccgg tgacatctac
    20041 tcggttgaag aaggaacggt cgtgttcccc ggtgaaccgc tcatgcgcgt tcacacgacg
    20101 atgatcgatg ccagcctgat cgagggaatc ctgctcaaca cccttaactt ccaaagcctc
    20161 atcgccacca aagcttcccg cgtctctctt gccgccaagc gcgggtcggt cttcgagttc
    20221 ggcctgcgcc gagcccaagg tcccgacggg gcgctttcgg caagcagggc ggcgtttatc
    20281 ggcggatgcc agtttaccag ccacaccttg gcgggaaagc tctatgatat tcccgtctcg
    20341 ggaacgatgg cccattcatg ggtgatggcg tttgattcgg aacttgaatc gttcagagca
    20401 tatgccgagc tctatcccgg atccactgtc cttttgatcg acacctacga caccctcggc
    20461 agcggaatcg aaaacgcaat catcgtcggc ctcgaggaga aggaaaaggg aaacaccatc
    20521 ggcgtccgga tcgacagcgg cgacctctcc tacctgccaa gggtgatccg caagcggctg
    20581 gatgacgccg gacttgagga tgcgttcatc gtcgtttcca acgatctgac ggaagagatc
    20641 atccagacgc tggtgcatga cggggttccc atcgacagct ggggaatcgg aacccacctc
    20701 gtcaccggcg gcatccaggc atccttgaac ggggtctaca agctcgccgc caaggagctt
    20761 cgagacggga gctatcttcc gacgatgaag atatccaact ccttcgaaaa gacgaccaat
    20821 cccggaatca agcagctcta ccgcttctac gacgctgaag gaggagcgat cgccgatctg
    20881 gtcacctgtt ttgacgaaga aatttcggtc ggaaacgact atgtcttcta ccacccgttc
    20941 gccgaaaccg aattcttcga gatgaagaaa gagcggtatg tgcggattga agcgttgctc
    21001 aatcacgtca tgaaagacgg aaagcgcctt ggtggaaaac cttgtctgaa aaccctgcaa
    21061 gaacggggaa tccgaagtct ggatacgatt cataaaacgt atttgaggca gatcaatccc
    21121 cacatctaca aggtgagctt gtcggagaaa ctcaaagcgc tgaagatcga gattcttgtc
    21181 tcgcaccgaa ggaaagccgc caagatcacg tagaaagaag cgcctcgatt gcatgaaaca
    21241 gcgcaagggc gatcgtctgt cgatactgcg gatcgaggag cagggccaac tccttgtcgt
    21301 tggaaagaaa gcccgcctcg accagaaccg ccggcatccg ggcggcgttg agcacccaca
    21361 aatccagctc tttgatcccg cggtttactt gcattggcag gcctttgacc aacgcactgc
    21421 tgaccacggt cgccaccttc ctcgtctcct caaagaggag gatgttgagt tcctttttat
    21481 tatgcttcga gaagagtgcg atatgctctt taggagtctt ggaatcgagg atgggaacct
    21541 ccttctcctt gagcttggtg aagacctcga acccttgggc ggcggggtgc tcggcgctgt
    21601 tgatgtgaat cgaaagcagg agggcgctcg tcttcggggg aagaaccgtc tgataggcga
    21661 ttctcccccg ctcttccaga gtaagaaacc ggtcatccct ccgggtcatc accacattca
    21721 gggacggatg ggcctcattc agcaaccgtt cgagacggag ggcgatatcc agcgtcagct
    21781 cccgttcgac gatcagcgaa ccttcaaaag agcggatcgc tccggggtcg tgcccgccgt
    21841 gaccggcgtc gatgacgatc gtgccgagcg gaagggcgaa gggattatcc accgaaaaga
    21901 gcggtgcggc aatgagacac atcagcagaa tgattgttcg aactattttt gccacgcggt
    21961 aaccaacccg tcatagtcga tcttctgcaa ccactgatgc cgctccctca cttccccccg
    22021 gtaggaggag cgatcgtagt tggccggtgg ttttctcact ttgttcttcc ctttcacccg
    22081 ccggatcgag aggttcttcg tataacagcg ctcaaatccg gtgcgctcgc ttcgatcttc
    22141 gataatcgat tcccgattgg gaaattgaat cagcaaatcg gggctgaggg ttatccgata
    22201 tccgaacaga tagctgcgca ggcccagatc catcagttga aagaaatcac tgtgaatcag
    22261 ctcgtcatag ccgcgaagcc gttggaagag ggcgcggtca taaaagccga gccccatcac
    22321 gggatagagg gtatgatcgg gtttgtccgg atccattgag ggaaagtcgg agccgggatc
    22381 gaggattttt tcaaaggggg tcggtgttcg cagacagggg acgatctcgc ggtacgcatt
    22441 cgcgatgatt gccgaagcga caaccgggtg gttgcttcga ctcatcagac tcaaaagccg
    22501 gtcgtattcg aaggttacca cgacggcgtc ggtgcgaaga agaagaaaat agttggtgtt
    22561 gcacaccgaa gcgacggcgt tcaccttctc tccgatgtag gtattgccgg aaaacacgat
    22621 caacgagatc cggcggtaga gttcctgaag ccgggggaag tcaaaccgcc cgtcctgagt
    22681 gatgacgaac aagggaactc ctggcatgct ccgctggtag agttcaatcg attctatgag
    22741 ttcttcagcg ctcccgctgt cgatcagggc gatcgccagc tggtcaacgc cgtaggaggt
    22801 gtgcatatca cgggcggaat agattcgata gttagtcgtc atgctccaag aataccatat
    22861 cttccggtcg gaagatcacc cctttgtgcg agttgcccaa aatggcggta aactccgacg
    22921 agtcatgacc ggcgacctgc ctcagatcgg cggaagaaaa cgaggacacc gcccttgcga
    22981 aaggagaacc atcgggcgtc cgcacttcaa tgatcgcccc ttcagcgaag gtgccgtcca
    23041 ccttcaccac ccccttgggc agcagacttt tctttctcaa cagagcgttc ttcgctccct
    23101 cgtcgatcac gatcgccccc tgaaaggagt tgttcaggat ccatcgctgg cgttggctga
    23161 gcgctttcgc cgggtggatg tacgttccca attgttcacc atccagaatc cgcaccatga
    23221 tatcggattc atatccgctg gcgatgatcg tcccgcaccc ggcgaacgaa gcgatcttcg
    23281 ccgcaaggag cttggttttc atcccgccgg tggcgtacgt cgaaccggca ccggaagcat
    23341 aggagaggat cgtcgaatca agggtgtcga tctcgctcaa caggttcgcc tcgggatcct
    23401 ttttgggatc cgcatcatag aggccgtcga tgtcggtgag gatgatcagc agctcggcgt
    23461 cgatcttgct cgcgatgaat gcgctcattc gatcgttgtc cccgaacgcg gagccgagcg
    23521 aatcgatgga aacgacgtca ttctcattga agatgggaac tacccccaat tccagaaggg
    23581 tcgaaacgct gttgcggagg ttcacgtatg tgcgccgatt gtttacatcc aagcgggtaa
    23641 gaaggatctg ggagcagatc agattgtggg ctttgaatgc tcgccggtaa gagctcatca
    23701 gcaatggatt cccgatcgag gcgcacgcct gacggagggc ggtctgtttc accggtcctt
    23761 tcaagcgaag ctctttcgcc cccatcccga tcgcgccgga ggagacaagc agcacctgat
    23821 accccttctc catcaatgcg gcgatctggg cggcgatcgt atcgatatac gcctcgtcaa
    23881 tccccgcctt cgtggagagc aggttcgtac cgacttttac gacgactcgt ttgacttgag
    23941 gaaggtcgcg cttcacaccg atcctccttg caccagggaa ggagcatcga cggaaagatt
    24001 cttgtggagg tatcgttttg catccttgcc gctgtaggca gccacgatct gacccgaacc
    24061 ggcaaggagg aacttcgtgc tcatcagccc atccaagccg accgggccgc gggcgtggat
    24121 cttcgttgtg gagattccca cctccgcccc gaagccgaac cggaacccgt cggcgaagcg
    24181 ggtcgaacag ttcgcaaaca cgtccgccga atcgacttcg gtgaaaaatc tcctgaccgc
    24241 ctcgggattc tcgctgacga tcgcatcggt gtggtgggac ccatatgttt cgatatgccc
    24301 gatcgcctct tccaaggaat cgacaacccg gatattcagc tccaatgaaa ggtattcgtt
    24361 gaaataatcg ccttcttcaa gcgggatgca ctcaatcgcc gcttgcgtcc gctcatcacc
    24421 gtgaatcgtc acgtttgctt ccttgagcgc cttgccgacc tccggcagga acacatcgac
    24481 aagcgaagca tgaaccagca gggtctcaat cgcgttgcac gccgccggat actgcgtctt
    24541 ggcatcgatg acgatcggaa gcgccttctt cagatccgcc tgctcgtcga tgtagaggtg
    24601 gcagatcccg tcgctgtggc cgaggacggg aatcgaggtg ttttccatca cgtaggtgac
    24661 gaacgcattg gatccgcggg ggatgagcag atcgatctca tcgttcatct tcagcatctc
    24721 tttgacgtcg ccgtggcttt caagcagggt gacatagcca tccccgaggg aggatccccg
    24781 gctgctttgt ttgatcgact ccaccagagc gaggttggtt cgtttcgctt cgctgccgcc
    24841 cttgaggatg atcgcgttcc cgcttttgat gcacagtccg acgatctgga ccagcgcatc
    24901 gggccgcgac tcgaagatca ttccgatcac tccgatcgga acggagatct gctccaggat
    24961 cagtccgtca tccagcagac gccgttggag aaccttgccg accgggtcgg ccaaagcggc
    25021 tacctgctca aggccgagga cgacctcgtt gagcttctgt tcgtcaaaga ccagacgttt
    25081 gatcaaggcg gcgccgatct gttgctcctt cgcttcagcg acatcctgct cgttggcttt
    25141 tcggatcgtc ggccaatccc gcctcaaccc tgcggctacc ttcccgagca gatcgatccg
    25201 ctcttcgttc gtgctcacgc cgaggactcg ggaactttct ttcagcgcct tgatccgctc
    25261 tttcacgctg tgactcatca tactcgtgct ccttacgcta gattacccgg aaggagccat
    25321 cggtatcaag gcagcctgcg ttgttttccc cttttaaact cggacgacga ttccctcgat
    25381 ctccttggag acccgcccga tgatcgccgc ttcgatgccg gccttacgca tgaccttcaa
    25441 tgcttcatgc gcctccccgg cgctcagagc ggcaagcagg cccccagagg tctgcggatc
    25501 gaagagaagc agctgcccgg tttcatcaag ctcatccagg ttggagatca ggtgcttgcg
    25561 gtgatcccgg ttggtatagc tgcccccggg gatgaagccc tgctcggcat agagccggac
    25621 tccctccatc aacgggacgt ccttcatgac aatttcaaac ccgttggggt tgtcgatcgc
    25681 catctcgctg aggtgtccga gcaggccgaa gccggtgacg tcggtgcatg catggacatc
    25741 gaactgcttg agaatctcgg ccgcatcctt gttgagggtg ctcatcacct tcaagaggcg
    25801 ttcctcatcc ccctttccgg cgaccttcgc tttcaccgcg ttctgcagga ttcccgaacc
    25861 cagcggtttt gtaaacagaa gaagatcccc gtcctgagcc cggttgttcg accacatctt
    25921 cttgggatga acggttccga cgacgcagag gccgaacgtc agctgttcat ctctgatgct
    25981 gtgtccgccg acaagggcgg ccccgctttc cttcaaggca tcaagagcgc cttcaaggat
    26041 tttggtaagg taagagctgt caagcttctc ttcgggaaag ctgacgatgc tcaccgccgt
    26101 gatcggcacg ccgcccatcg catagatgtc gctgatggcg ttggtggcgg cgatcttgcc
    26161 gaagaagaag ggatcgtcac acaccggcgg aaagaaatcc accgtctgga cgatcgcaag
    26221 atcgtcactg agcttgtaca cccccgcatc ctcggcgtga ttgaaatcgc tgagcacgtt
    26281 gggattatac ggctggacaa gcccgcagag cgctttgtca agcatggcgg gggtcacttt
    26341 cgccccacag ccggcgaact tggaaaatgc tgtcaattgt acttcttttt tcatgaaatc
    26401 ctccgaaatg cttttgcccg catcccgtcg atgacctcca ataccgatcc accgagggcc
    26461 cgggccttgt cactgatcgt ctcgcagtat gatgcgatgc cgcgcgggtc tatatccgcc
    26521 accttgaagt tcgttggaac gacgattccg tcgtgcagca tcccccggac caccccgtcg
    26581 atcggagcga tgatctcatc cgttccgacc gctgcgatca cgtcgccctg tttgaccaag
    26641 tcaccgatct tcttatggcc gacaaacgtg cctgcgctcg cagaatgcag aactcgttcc
    26701 tcagcaaagc cgccgatctt ccccggaatt ccggtattcg gttcggcgaa tccccgactg
    26761 atgaccttcc cgaggtcatg cccccgcttc gtttcgatca cgtaatcgca atcgaccccc
    26821 gcttcaaagc cgggtccgag ggcgatgacc agcggcgcca tccctttgtg agttcccagg
    26881 ttcttcttgg cgatgatcgc atcgacgacc gcgaagggtc gaagacgttc gatcatcgaa
    26941 gcgctcgggt cgaccatcag tgcgacgatc ccctgatcga gcagcgtctt cgcctcctgg
    27001 tcgctctcgg ccctccggca gactaccgtt tcaagcgcca cttccccggt gtacaccgct
    27061 tcgctgaagg cgacggttcg gcggatcgtc gtcggctgct cgatttcaag ggcgcatacc
    27121 cggtatcccg ccttggcaag ccggacgatc acgccggtgg ccagatcccc cgccccgcgg
    27181 accacgacga ggttttccgc tttcctgctg agactctggc cggatcggtc atggagcacc
    27241 atcatcacct cggccatcac ggcgatcgcg atctcatgag gggtctcgga tccgatatcc
    27301 agaccgaccg ggctgtagaa atggtccaag cgttcgatgg aaatgtttcg ctcactctcc
    27361 agggctcttc gataggtgtt cactttggtc cgggaaccca gcattccgat gtaggcggcg
    27421 tcgctggtga tgaccgcttc caatacggat ttatccaagc cgtgggtcgc tatgacgatg
    27481 gcgcaatccc gatcgatctc aaggctcttc atcgcctcct cgaccgtctc ccccacatgg
    27541 aacgccccgg catcgggaaa gcgttcgccg gtcgcatact ccgcccgggt ttcgatcact
    27601 tcgatcctga acccgcatcc cgctccgagg cgggcgatct gttcattcac atgccccccg
    27661 ccaaacaaca gcaggcggcg cttgcttgca atcacatcca cgaaaaactc aagcgttcca
    27721 ccgcaggcat gaccgtcggt gacggccagc gagacatcca catgggtgct ttttccttca
    27781 gcgatcgcgg cgaccgcttc cttggtcacg gtgaactcgg cgatcccccc gccgacggtt
    27841 ccgatcgtac tcccatcttt tttgacgatc atgtgggcgt tgctccgcgg tgtggagccg
    27901 acgcttttgg tgatcgacac gaaggcgaac gggatattcc gatcctgcag atccgctgct
    27961 tgttcaaata tgttcatact tcacctcgac aggtcccggc aaggactgct gtaattcgtt
    28021 ttttggttcg acccagacga agggcttcgg cgacgacctc gtcacgtgcg gcatcgcttt
    28081 gattatagac caggatgacc ggatgtgccg aggttccttt cagaatcccg tcgggatggt
    28141 caagcaggac atcaagcgta tcgagggtga tccacgcttc cttcgagcct gtggcagcgc
    28201 gatattcccc cacgcgatgg aaggtctcct cgtccagccg cttcccgatt ccgctcagtc
    28261 cgacgacggc gatgacgcaa tcgacccatg gaggaatcac cggttctccc tcattgtgaa
    28321 atttgaacgg tcttctccgc gccccgtccg cttcgatgag ggtgtatccc gccagcttgc
    28381 tcgcctcttg gagcagctcg ggatccacgc tcccgagctt gtccgaatcg ggtgccgccc
    28441 agagcacctg acgaccgggc tccaccttcg gcggattgtt cggatcgatt gcgatctcat
    28501 cgaacgggta ctccatatcg cgcggtgaag ccagatgggt cgtcgttgtg atgagcaccc
    28561 cctcttcctt gaattggtcc gccaaaccga tgatgatgct tgtcttcccg ccgcttccgg
    28621 taacggcgat tcgtctggcg gaaagcgatt cgatgtgagt tccgagttca tcaaagaggg
    28681 aaaaagtcat acacccaagt ctaatcgcct gccattatac agtcaattat aaaatggaaa
    28741 aattcgattg tatttttaaa tacataatgg tacgcttaca gcacaccaag gaggaatcaa
    28801 tgaaacgtct tatgcttatc gcaaccggcc tgttgctggc cgcagccctc attgcactgc
    28861 ctgcaagcga agcggcaccg aagactcttt cctacagcgc caccgatgca agcgggagaa
    28921 ccatcaccct cgacggcaaa ccggaaaagg ttcttgtcgc cgccaaagcc gcgctcatgc
    28981 cggctcttgc gctctttttg ttcccggaag cgcaggagat ggagatcagc ctttccaaga
    29041 ccgaccaggg gctcggggat ttctttcccc tcgtctggcc gcacatcaat cccggcggaa
    29101 ggctcgacca gaacgcaagc gtcgaggagc tgatcacgaa gcaaagcgat ctcatcctgt
    29161 tgaaagcgac ccactatgaa agcatgggca aaaagctcga ccaggtcgga gtcaagaact
    29221 ttacgttgag tcttgaaaca tgggatgagt ggcaggccga actgatccag ctcggaaaac
    29281 tgctgggctc ggaagagcgc gcccgacacg tcctcgacgt atatgaaaaa cgtatcgccg
    29341 cacttaaaca gcgggtcgcc cacctgtcgg atgaccaaaa gcccgacgtc cttcttctcc
    29401 agggagctcg gagcgacaat gcctattcct ttaaaatcgc tcccgattcg tggctccaga
    29461 cctgggtcgt cgaattggtc ggaggaaacc cggtctggaa gggatccaac aaagcggcaa
    29521 gcggctggtc ggtggtgacc ttcgagcaga ttgcagcatg ggatcccgat ctcattgtta
    29581 ccatcagcta caacaccccc gcttccatct atcaggatgc gatccttgac tcgccggtgt
    29641 ggaaggagct cgccgcggta cgaaccggca aggtgtatgc aagcccctcc gatctgatga
    29701 actacattca accggtggca tcgtggatcc tcggcgccca atggctggca aagacgatcc
    29761 atcctgagct cttttccgat ctggacatgg atgccgaaat ccgcagcttc tacacggatt
    29821 tctacgggat caccgacatg acgctgctcg acgaattcgt ttcgcgcttt gaaaagtcgg
    29881 tggtgaacaa tcgatgagcg agtacgaaaa gcaaaaacgc caaaagatga tcacgctcac
    29941 ggttctcctc gccttgagcg tgctctttgg catcctctct ctgtttctcg gccggtaccc
    30001 gaaagcgggg ttcacttcgc ccgttctgtt ttataccgat tcagtcgccc ggacgattct
    30061 taccaaaatc cggcttcctc gggtcgtctt ggcgctcgct tccggagcga tgctcggggc
    30121 aagcggcttc acctttcaat tgctcttctc caacccgctc gttgagcctg gcttcctcgg
    30181 agtaagccag ggagcagcgt tcggagcggc cctgatgatc atcttcgccg gcgcatcttc
    30241 gctctccatc cagctttcag cgatgatctt cggactcctg ggattggtgt tttcctatta
    30301 tctttccacc cgatttcgtt ttggaggacc attgttgcgc ctcgtcttgg cggggatcgc
    30361 cgtctcggcc ttcttctcca gcggcttggg ggtgatcaag ctcgtagccg aaccgacgaa
    30421 ggatcttcag gacatcacct tttggatgat gggcggactc tggaacgctt cctggggttc
    30481 tatcattccg actttggtcg taatagcgat cgcgttgacg atcctccata tgaatcgttt
    30541 caccctcaat cttctttcaa tcaatgaaaa aacttctttt tctctggggc tcaatccaaa
    30601 acgtcagcga gtcatcctgc tcgtcgtcgc cacggtggga acaacgttca caatcagcgt
    30661 gaccggcctc atcggatggg tcgggctgat cacccctcat atcgcccgaa agaccttcgg
    30721 cagcgaagcg acccacgccc ttcccgcctc gatggtcatg ggtgccttct tcctgctttt
    30781 atgtgattcc atcgggagaa cgattcttcc gacggaaata cccatcggat tattgaccag
    30841 tctcttcggc gcgttgatct tcgtcctcat cctttccggc aaaagccagg agggcaaggc
    30901 atgacgttcc tgtcaataga aaaactctcc tatcaatacc ccaatggaat caatgcgctt
    30961 caatccgtta cgctttcaat tgacaaaggc tcctccgttg cgatcctcgg atccaacggg
    31021 gcgggaaaat caacgttgct cgatatactt ttaggataca aaccgagcac ttcggtttct
    31081 ctttatggca aaccgctttc gacgtatggc cggaaggagt tgggtcggct catcgccctt
    31141 gtcccccaaa aggagcagtt ctcttttgcc tacaccctcc tcgattacac cctcttcggt
    31201 cgggctccat acctttcccc gatgggcatc cccacaagca gcgataacct gatcgcctat
    31261 gaagcgttgg cgaaagtcgg cctcggcggc tttgaaaacc ggagcatcac cgcgctttcc
    31321 ggaggagagc agcagctcct catcatcgcc cgggcgatcg cccaggaatc ggagatcctg
    31381 ctgctcgacg aaccgacctc cagcctggat ccggcgaatc ggaaacggat tctcaccctg
    31441 ctcaagcaac tgcattccga ggggaaaaca ctcatcttca cgacccatga cgcaaacttt
    31501 gcgtatgagc tctcaagcga tatcgctttg ttgaaaggcg gcacgttgct tggctacggc
    31561 aagaaagaag aagtggtcaa aagcgaactt ctgaccacgt tgtacgatac tcaactaacc
    31621 gtaaccgaga tcgacggtcg gaccatcatc tattgatcca gctccaaatc gacgaagctg
    31681 aacgttccga cgtcaaagag gcccatgtcg gtcagcttat acgtcgggat gaccggcagc
    31741 gccatgaagc tcagcgtggt gaacgcatcg atgccgggtt tcacggaaag tttctcaatc
    31801 gccatgtcgt ggaggtgttg cagcttctct gcgaccacct cgcccttctc atagctgatg
    31861 aggccggcta ccggcatctc cagagtggaa agcacctttc cgttttgaat gacggtcatt
    31921 ccgccgcccg tttcgatcaa gtgttcgacg gctttggcca tatccgcatc gttgtcgccg
    31981 gcgacgatga ggttatgcga atcatgtccg accgttgaag caatcgctcc tttgcgcagt
    32041 ccgaatccgc ggattaatcc gactccgacc gttcccagtc ccttgtgccg ctcgatgacc
    32101 gcaagcttga ccacatcctg gttgggatcg tggacccaca cacctttctc cagcttgacg
    32161 aacgcctcac cctgttccgt caccactccg ccggggacga tgtcgatgac ccggacccgg
    32221 ttgtgcttca accgaagatc aagacgctca acggaaaaat ctttgatgtt catcttcgat
    32281 gcgacgcgct gatccttctt cgcttttgat ggcttgagca tctttccttc ctgagcgaca
    32341 agaactccat ccacatatac ttggtgcatc ctgaattcaa caagatcatc agcaaggcag
    32401 aagtccgccc gtcttccggg gacgatcgcc ccgcgatcgg tcagattgta gcactcgccg
    32461 gagttgatcg tcgccatgca gatcgcttcg atcgggtcga gaccttcatg gacggcgata
    32521 cgaagattgt tgttgatatg tccttcttca aggatgctga ccggctgccg atcatcggtg
    32581 cagaaacagc agcggcggct gttcttcgaa tcgactcccc ccagcagccc gatgacgttg
    32641 ttgcacgccg agccttcccg aaggtgcacg tacatccctc ttcggacccg atctctcaat
    32701 tcagcgggag tttcacattc atggtccgtt ttgattccgc ttgcgctgta cgcatccaaa
    32761 tccgcgtcat agaccgccgg actgtgtccg tcgatgactt tttccgcttc ttttgcaacc
    32821 atgattttgt cgatcaccag atcttttcct gcgatcactc cgggataatc catcatctca
    32881 ccaagtccga caatgcgctc atggtccatg acgcttctga tgtcttcagc ggtgattacc
    32941 gccccggcgt gttcaaaact caccgcgggg acacaggagg gaacgacaaa aaacatctgc
    33001 aacgcgctct ctttgctcgc ttcaagcata tagtgcaatc cgtcaagccc ggcaacattg
    33061 cagatttcat gcgggtcggc gatcaccgtg gtcgttccgt gaggaacgac gagattggaa
    33121 aactcctcag gggtggtatg actcgattcg atgtggacat ggctgtcgat cagccccggg
    33181 atgagaaagc ggcctccccc atcgacgatc ttcttcgctt tccccttacc gggaggtgcg
    33241 aagccggcga tatggccctc gctgattatg aggtctgctt caaaccattc tttattgaat
    33301 acatcgagaa tatgtgcgtt tgtgatgcac aagtccgcgc tctttctccg cgcactggca
    33361 gcaatgaggt tttgcaactt ctttttatcc atcggaacct ccttcatggt tccttacagt
    33421 gtaccacgtc gccggtcatc ttcaaggcaa aaccgtgttt tttcacatct ttcttaacgt
    33481 gagaacgccc ggtttttgtt gtatctgttg cagtattttt tatttcatgt attcaaatac
    33541 aacatatttg ttgcggattt ttaccgtttt acgagacttg accttgtgta aaccaatcgt
    33601 tgtgaagtgt caaaatcaac tatttctcaa tttctgtcaa catacctccg aattccccaa
    33661 tttatgcgaa atttacgaca attttgatga tttatctgta tttattcccg ctaatgaggt
    33721 agaatggagg tatcaaaaca tctgcaagga gcgaatagat ggacaagttc tttaaaatta
    33781 cagaacgaaa atcgaccgtc aaaacggaag tcatcgcagg aattacaacg ttcctcacca
    33841 tggcttacat tctggtggtc aatccgggca ttctgtctgc cactggaatg tcgtttgaga
    33901 aagtattttc agcgacggca atttcatccg ccatcgccac gctgatcatg gcattgatgg
    33961 caaaccttcc ctttgccttg gctcccggaa tgggactgaa cgcgttcttt gccttcagcg
    34021 tcgtcctcgg catggggtat tcttggcagt tcgccctcac ggcggtcttc gttgaaggta
    34081 tcatcttcat catcctgacc ttcgtaaaca tccgggaggc gattgtcaac agcattccnn
    34141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ttcatcatcc tgaccttcgt
    34261 aaacatccgg gaggcgattg tcaacagcat tcccgcaaac ctgaaaaaag cgatcggggt
    34321 cggtattgga ctcttcattg ccttcatcgg cttgcagaac gccggaatca tcgtcaacaa
    34381 cgatgcgact ctcgttgctc tcaacccggg atggttcaag ggcggtccgg gcttggcgat
    34441 gctcggcctt ttaatcaccg gagtcctcct tgcattcaag gtcaggggcg cgctcttgat
    34501 cggtatcatc atcacaacca tcatcggcat ccccttcggc atcacccaat atgccggcgg
    34561 caagtatgtt cctccggcgc cgtatttctt ccaattcgag ttctcgaaag cgatattcag
    34621 tcttgatttc gtcgtcgtca tgttcacctt cctcttcgtc gacatgttcg acacggtcgg
    34681 aacgctgatc ggatgcgcaa cgaaagcgga tatgattcag aaagacggct cgattccgaa
    34741 cgtcaaggaa gcgctgttcg ccgatgcggt cggaaccacc gtcggcgcca tcctcggaac
    34801 ctccaccgtc acgacgttcg tcgaatcttc ggcgggtgtc gtcgaaggag gacgaaccgg
    34861 attgaccgct ctcacgaccg ccattctctt ccttctctcc ctcttcctcg taccgctctt
    34921 cagctcgatt ccgagtgttg cgaccgcccc ggccctgatc atcgtcggag tcttcatgat
    34981 caccccggtt gtcgatatcg actgggacga catgacggaa gcgattcccg cattcttgac
    35041 ccttctcttc atgatcgttg catactcgat cgccgaaggc atcatgttcg gtatcatctc
    35101 ctacgtcctt ctgaaactct tctccaagaa aggcaaggaa atcggtggaa tgacatgggt
    35161 cgtcttcgcg ctcttcgtag cgaagatctt ctttggtgct cttggaagct gatcacctca
    35221 acaacacatt caggtggacc gatcggtcca cctttttttg cctcttagcg tctttcatca
    35281 atatgtattc ttggctacaa tgagcccatg acaccgcttg attccacaaa agaatatttc
    35341 gttacgggag ttgccggatt tatcggcttt catcttgcgt catccctgct caaacaaggc
    35401 tgtaaagtca tcggccttga taacctcaac tcctattatg atgtccggct caagcgcgag
    35461 cggctgaagc tgctcggttg tgaacgcttc accttttatc atgccgacct gaccgataag
    35521 aagaagcttg aaacgatctt tcaacgtcat ccgatcaccc acgtggtcaa cctcgcagcg
    35581 caggcgggag tgcgctattc gatcgaccat cccgatgcct atatcgattc aaacattcag
    35641 gggtttctga acgttctgga agcgatccgc acccacccgg tcgaacatct cgtgtatgca
    35701 tcctcctcct cggtatatgg aaaaaacacg aaaatcccct actcgatcca tgaccggacc
    35761 gacgagccgg tgagcttgta tgcggcaacc aagaaagcca acgaactctt cgcccacacc
    35821 taccagcacc tctattcgat caaaacgacg ggggtccgct tcttcaccgt ctatggcccc
    35881 tggggtcggc ccgatatggc gtactactct tttaccaaga aaattctgaa taacgaaacg
    35941 atcgatgtgt acaacaacgg tgacatgtgg cgggacttca cctacatcga tgatgtgatc
    36001 aatgctctgg agcatatcat cccacgtgtt ccaaccgatc cggtcatcta caatatcgga
    36061 aacaacaacc cgatccgcct cttgcgcttc atcgaagcgc ttgaagaggt tatcggcaag
    36121 aaggcaaaca aaaacttcct gccgatgcag gacggggatg tctaccaaac gtatgccgac
    36181 atctccgatc tccagcggga tttcggcatc accatcaaca ccccgatcga agagggactt
    36241 gcttccttcg ttgcgtggta tcgttcttat tacaacgttt aaaaatgtat gacgtgatcg
    36301 tggttggagg gggcgttgcc ggtctcttcc tcgctgcaaa cctccccgat cgaaaggtac
    36361 tgattcttga ccatgccaaa tcggtgggca aaaagcttct gatcaccgga ggagggaggg
    36421 ccaacatcac caatgacctc tcggcaaaag aaatgcttgc ccactttccg acgaaagcga
    36481 tgcggaactt tctcgcccct tccctgctga gtctcactcc ttcggatctg atgaattggt
    36541 tcgagaatcg aggagtcgag ctcatagtca gagcggatgg acatgtgttt ccctcctccc
    36601 tcgatgcccg aacgatacgc aatgctcttc tttcccacat ccatgcggat attcgcaccg
    36661 cttgcacggt tgcagcgatc atcaacgaag aagaatcgtt tacgatccac tcatccgagg
    36721 gatcgtttgt cacccggtcc gtttgcctgg ccaccggagg aatgagctac ccctcaaccg
    36781 gcagcgatgg cagcggatac gccttggcga aggcgctcgg tcacacgatc gtcccgccaa
    36841 agccggccct cactccgatt gtcacctgtg aacagacagc tcacctcagc ggcatttcgc
    36901 tgtccaacgt ctccattacc catagacaat tcactgcaac cggcgatctt ctcttcaccc
    36961 gtgacgggct cagcggaccg gtcatcctca atctttcacg agcggtgagt aaaggcgatc
    37021 cggtgacaat tgccgttctt gattcctctc tccttgaaga gaacctcctg tcctttcccc
    37081 aaaaacgggt ctcaacgatc atcaggacat tgggagtacc cgcatcgttg gcaagggaga
    37141 tctgcgagcg atcccacctt gagcccgacc ggaatgccgg cacgctccga aaagaggagc
    37201 ggaagctcct gtgtaaaaca gcgggtgcat taacttacaa ggtgaacacc accaaagggt
    37261 tcaagcaggc gatggcgacc tccggcggag tcgagctttc cgaggtcaac agaaaaacga
    37321 tggaatcgag gctaaccccc ggcctcttct tctgcgggga ggttctcgac tatgacggag
    37381 aaagcggcgg ctttaacatc caagcggcat gtagcaccgc gtacctggct gcccggacga
    37441 taggaaaaag aacataacat catggacatc gatagcgtag aacaaggagc ctttcggctc
    37501 cttgtcggtc gataacgttg aatcgttgcg cttatttgat ccgctgcttt cccttttcaa
    37561 ggatttgctc aagcttggcc gccgggtctt tgaagcgggc gaggaagatc atcgccgcga
    37621 tgacgtaggg cttgtagctc gcttccttgt agaggtcgac gatgtatcga tcgaacaccg
    37681 atgcttcgac ttccccttcc ttgcaggaga cgccggtgat gtcggcgggc agacagtgca
    37741 tgtagagcgc cttgccgtcc ttggtggtgg ccatcagctc ttcggtgcag gtccaatcct
    37801 tgaacttggc gttgttcagc aggcagcgct gctccagttc cttcagcccg gcctggtcgt
    37861 tgtgctcgac gatctcggtc cgctcttcca tcaccgcgaa cggcgcccag ctcttcggat
    37921 agacgatgtc ggcatccttg aacgcttcgg ccatcgtgtc gacccgcttg tatgatccac
    37981 cgttggattc ggcgttcttc ctagcgactt cctcgacctc gctcatcacg ttgtaccctt
    38041 cggggtgagc gagcacgaca tccatcccga agcgggtgaa gagtccgatg actccctgag
    38101 gaacggagag cggcttgccg taggaggggc tgtacgccca ggtcatcgcc actttcttgc
    38161 ccttgaggtt ctccacgcca ccgaagtggt tgattacatg gagcatgtcc gccatcgtct
    38221 gcgtcgggtg gtcgatgtcg cattggaggt tcaccagcgt cggccgctgt tccagcaccc
    38281 cctggtcgaa cccttcctgg acggcggagg cgacttcctt catataggtg tggcctttgc
    38341 cgatgtacat gtcatcccgg atcccgatca catccgccat gaagctgatc atgttggcgg
    38401 tttcccgaac ggtctcgccg tgggcgatct ggctctgctc ctcattcaga tcctgaacct
    38461 ccaagccaag caggttacag gcgcttgcaa agctgaaccg ggttcgcgtt gaattatcgc
    38521 ggaagttgga aattccaaga ccgcttgaaa acacttttgg cgagatgttc gcttcccgca
    38581 tcgcgcgaag aatttcggcg acttgaaaca ctgcggcaag ttcatcatcg gttttgtccc
    38641 aggtaaggaa gaagtcgtcg agatacatcc cttcgctctt cagcgttttg agcttctcga
    38701 tcattgtttt aatggcggtt ttgtctttca ttcatgcagc ctccgtggta atgttccgaa
    38761 aaaaatccgg acaataatac tctaccacag cggaatatta ttgtccagaa atttgttgca
    38821 gtttgttgca ttcaagggga tcagagcttg cccatctccc tgagtaactt ctcgggagtg
    38881 aagggccatg acctcatcca aactcctact gcatcatgaa ttgcaaccgc gatcgccggg
    38941 gctgccccgt tggtcgaaat ctcactgatc gatttcgctc catagggccc gacctcgtca
    39001 tcgacgtcga tcaggatcgc cttgaaatcc tccggctgct cggtgatcat cggcacgccg
    39061 taatccccca ggcggacgtt ctggatatac cccttctcat cgatcgtcat atcctcgtag
    39121 agggtgtggc cgatcgactt caacgccgct ccgtacatct ggcagagcgc catctccgga
    39181 ttgatcgggg ttcccgcgtc ctgaagggcg tagtacttct cgaccttgat ctgcccggtt
    39241 cgggtgttga ccgcgacctg gacaaaatga gctccgtacg gaatcgagtt gtgctccgta
    39301 acgaaggaac ccttgcccat cacctggccg cgaccgtcgc cctgtaacgc attatacccc
    39361 agcgtggcgt aggagaccga tgctcccgtt ttggtcgagt acacctcccc cggggcacga
    39421 agcgcgagat cttcggcctg ctcgccgagc tggtgggcgg cttcatcgag gatgttccgc
    39481 ttcagatctt cgatcgcata gtaggtggca ttgccgctga agtacgtccc cgaagatgca
    39541 tatgctccgg tatcgaaggg acagctgtcc gtatcaccgc tggtaacgtt gacttggtca
    39601 agggtgacga agaggccttc ggccgcgatc ttggcatgaa tcgtatccaa accggttccg
    39661 atatccgctc cgccggaaaa gagctggatg ttcccgtcgg gaagcagttt cgcccatgca
    39721 ttgctgtggt cgagacccgg aagaccgcta ccctgctgaa tgatggcaaa gcctttaccg
    39781 atcttccaat cccctccggg acccttttcc ttcttgcccc agtcgatcat cctcgcgccc
    39841 ttggcaagcg cttcaccaag accgcaggag ccgacgggaa cgaccttgcc ctctcggcct
    39901 tcaccgaggc cgcggaggat ttcaagcata tagcccgact cgattttgtt cttgtccacc
    39961 agatcgtagt aatcgatccc aagcccatcc gccaattccg caagagcggt catcatggca
    40021 taggtgcctt taggggcgcc atagccctga tatgctccgg tcggcgggat gttggtgtaa
    40081 taggtgtaga cgtcgaattt catgttgtcg cacttaagga gcggcagcgt cttcgaacag
    40141 gcgttcatcg gaacggtcaa acagtggttt ccatacggac cggtgttcgc ccggacatcc
    40201 atgtatactc cggtgagcgt tccatccttc ttcgctccga gcttcatcgt gatccgcatc
    40261 gggtgccggg tggagtttgc gatgaactcc tcttcccggg tattccgata gtagacgggt
    40321 ttgccggtca gccaggtcgc atacccgacg agatcttcaa cgagaatatc ctgcttggaa
    40381 ccatacccgc cgccgacccg ttccttgatc acccgcacct tgtgttcggg aatctcgcac
    40441 acccgggcga caatccgccg gacgtggtag ggaacctgcg ttgaggcgtg aagaaccagt
    40501 cttcctccat caatcttggc gtagcagacg tgcggctcca acggcgtgca ttggatctgg
    40561 ttggtctggt aggtgcgctc gatcacgaga tccgcttcct tgaacccctt ctcgacatcc
    40621 ccgatctccc cgtgggcggc gctggcgatg ttccgtcgga tgtccccgtg gagcgggaac
    40681 tgatagatca ccttgccatc ccggggatcg gcgcccttgt tgtattcatc gagcccctcg
    40741 ggagcgccga cgacgtattc gacgaccgag ttgtgcacca gactcgctcc ctcggccatc
    40801 gcctcctcga cggtgaacac cggcttgagc acttcgtacg tgaccttgat cagcttcagc
    40861 gcctcgcgag cgatttcaat ggtttccgca acgaccgcgg cgacccggtc accgacgtgc
    40921 cgaaccttcc tgttcatcag cttgcggtcg tacgggctgg gttcggggtt cccttggccg
    40981 gcctgcatat agtacacgtc cggcacgttc ttgtgggtga tgaccgccac caccccctcc
    41041 agcgcctcgg ctgtggcggt gtcgatctcg gtgatgtacg ccatcgcatg ggggcttcga
    41101 agaatcgcca tcgaatagta gcccgcctga agccggtctt ccacgaacgc cgcctctccg
    41161 gcgacgaggg tcggtccgtc gactttccct ttcggctttc cgacatacgt gagatggtcg
    41221 cggaacgtgg ggctgatcgg ggtcttgtac ttcccgaatt cgatcagctc gcgggccagc
    41281 tgaaccgcaa gatagtaatg ctcgtatccc gcatcccgga tgaagatgcc gcttaaaacc
    41341 tctttgatcc gctcacgggt gggattgggt tcatgcttca gaagccaggt cagcagcaac
    41401 gccgcctgag gggcgttgta ggcgctctgg acgactcccg catcgatcat cgcccgttgg
    41461 acgggattca ggttccggct gtttcccatc gaatcggcgg tgaggatctc tcctccgtcc
    41521 gcctgaaccg cattcatcag gtttgcgtac acgggcagat cgttgaagat gatcgtatcg
    41581 ctgccggcaa atccgtcccc gtcatcgctg tcgcggacgt tggtatatcc gaggcgaacc
    41641 agcatcgccc gcaatgtttc agagccatca acgtcaaccc tgtgtctttt tccgtttacc
    41701 gtgaattgaa tggtcatcgt tggcctcctt tgagaaattc accatagagc atcgcgactc
    41761 cttcaccggc gatatagcgc ttgtactcgc cgctgccggt gtagtcgctc ttcaccgtta
    41821 cttccgccgc aaccgacttg gcgatctcct cttcggtggt gatttcacct ttctcgatcc
    41881 tgctctcggt ctccttgagc cgcagcactc cgacggtgta gaccgcggcg tacacgcgga
    41941 ccttcgaacg atccttctcc gctccgaagc ccaccgtgac tgaagcccgg ttctggctgc
    42001 tcaccgcaaa gcggagcgtc ccgacgaacc ggtcgctttt cctgatcttg aatcccagga
    42061 tcagggctcc ctgataggag gcgaagtgct cgtggtactc acaagccggc acttcatcct
    42121 cggcgtactt gcccttttca ttaattgtag cgatcaggag gatcgccctg cttgccagca
    42181 gaaccggccc gacatacgag cgaccgagca tcaacgcgaa gttccccccg atcgtcgcca
    42241 tgttccgccg ggtgaaggat ccgcagaaga gcgcagcctc cttcaaccag gcgggaacca
    42301 ccgaactggt cgcgatcgtc tgcagggtcg ccatcgaacc gatgatgatc tcatcttttt
    42361 tctcttcaat cctgttcagt ccaagcttgg aaagatcgat cgccacctcc gcctgcaccg
    42421 cggattccag gcggttgatc tccgtccctc cggcgtagaa cacactcttt gcatgtaagc
    42481 gtttcaggct caacgcttcc tctttacttt gtgctacgag gaatttcttc atcataacgc
    42541 ctcctttcgc gctgctcgat caagcattcg cttgctgatc gcattcgctt tttcggccag
    42601 tagcgcttca tcgaatccga ccagggttcc ccggtcgacc accaccttgc cgttgatgat
    42661 cgtataatca gtgtaatggc tgtcgccgca gaagagaaga gccgcaacgg ggtcactttg
    42721 agctccggca tacgccgcag tcgagagatc ccagatcgcg gcatcggcgg cccacccctc
    42781 ttcaagcttg ccgacgttgg tgaagttcag catcttcgcc ccgttgattg ttcccatcgt
    42841 gaacgcttct tttgcggtaa gcgcatcgct tccgtagcga acccgctgca gcagcagcgc
    42901 ctggcgtact tcggcgagca tgtcggatga atcgttcgaa gcggatccgt cgacggcgat
    42961 gccgatgttg atccccatcg gaagcatctc ggcgacccgg cagatgccgc ttcccaggcg
    43021 catgttggat gaggggcagt gggcgatgtg gctgcccgtg tcagcgatga tcttcagctc
    43081 ctcgtcgttg aagtagatcc cgtgggcgta atacacgtct tcaccgatca ggtcgcactc
    43141 ctgcatcagt tcgacgggtc tcatcccgta catctggagg cagaaatcat tttcatcggc
    43201 ggtctcgcaa aggtgggtat gaagcctggc tccgtacttc ctcgccaagg ccgctgtttc
    43261 cttcatcagt tcctttgtaa ccgaaaaggg gctgcaggga gcgagggcga ctttccgcat
    43321 cgcatcggcg ctgctgtcgt ggaagcgccg gatcgagtct tcactgtggc gaaggatctg
    43381 ctcttcggtc tgcaccacgg tatcaggcgg cagccccccg tccttcttgg agaggctcat
    43441 cgaaccccgg gtgggactga agcgcattcc gagcgtctcg gccgcctcga actggatcgc
    43501 catgggatcg ccttggacat ggtggggata gacgtagtga tggtcggtgg tgagggtgca
    43561 ccccgttctc atcagctccg ccatcgccag aagcgaggaa tagtagatgc cctcttcatc
    43621 gagatatttc cagacatcgt agaggaacgt cagccaatcg aagagcttgg cgttctgaac
    43681 cgccggatgg tttcgggtca aggtttgata aaagtggtgg tgggtgttta caagccccgg
    43741 cagaacgacg tgcttcgaac agtcgatcac ccgatcgacg cccttggagg agatctttcc
    43801 cgcaggatcg atcctgccta ctttgttgtc gatgataagc agatcggcgc cgtggagggc
    43861 ctccttgtcg aagctcgttt gaagggtgta aatatttttc aacaaaagcg aggaactcat
    43921 gccatttctc cctatagagt cgtggtcgta gcggcatgcg ctgctgcgca aggaaggtga
    43981 tggggtgagg agtccttcag caccttgatt tccacgcccg cccaatgcgg ctccccgtgg
    44041 attccattat ctaaatacat cgataatgga gtcttctcat aagacaacca caaggccgaa
    44101 gccctgtggt tgccgattgc caaaaatcaa agaggttatt ttcctcgtgc ctcaagggag
    44161 tacggcagca tcgcatagaa cgccgcacag atctcaaggt catccacccg gttgatctcg
    44221 ttcggtgcgt gagcctgatt ctcgtcaccg gggccgaaac cgatcgccgg aatcttgtgc
    44281 cggccggtgg tcgctactag gttcgtggag aacgtccact tgtcgacgac cggctttttc
    44341 ccgaagagcg tcttgtacgc atcgattccg ctctgaacga ggaagtgatc ctcatcgagc
    44401 ttccacgtcg ggaaatagag ctcctgcgaa tactccttct tcgtccaacc gatcttctcg
    44461 tagttgggca tctcgacgat gaaggaatcg ggagcgtcgc cggtggcttt ggagatgtac
    44521 tcccgaacct gggcgatcgc catgtcggca tcttcacccc acgtcaggcg gcggtccaga
    44581 tagagcatcg catagtcggc gacggcgcac tgggaaggtc ccttgacatc catcacgctg
    44641 acggtgatcg ttccctttcc gaggaagccg tcctcatccg gctggagatc cttgttcagc
    44701 tgctccatcg caagggcggc gcgggctgcc ttgtaggcgg cgctcactcc gcgctcgggg
    44761 gcggagccgt gacaggagat cccgctcaag atgacccgga tctccattct tccgcggtgt
    44821 ccgcgataga ggcggcagct cgtcggctcg gtggaaacga ccagatcggg cttgaggcct
    44881 tcctcttcga tcagatactt ccagcacatt ccgtcacagt cctcttccat cacggtgaag
    44941 gtgaagtaca cggtatactc gccgccgtag cccagctcct tgaggatccg gccggcggtg
    45001 atcattgaag ccgccccgcc cttctggtcg gatgcgcctc gaccgtagac gataccgtcc
    45061 ttgatctccc cgctgaacgg gtcgaaattc cacagatcgg ggtttcccac ttcaacggtg
    45121 tcaatatgag catcaaaggc cagcttcttc ggcccgtttc ccactcggcc gatcaccgag
    45181 ccgaggccat cgatgtatac ctcatcaaag ccggcttcct ccagcagggt aacaatcagg
    45241 cgggctacat cttcctcttg ggagctgtag ctcttcgtct tcaccatttt cgagaggttc
    45301 tcagcggtgt aggagcggag ttctttcgct cgagctttga tcttttcatg catgttcatc
    45361 gttgtaccta ccttcctttt cgtatatatt cttcgccttg ttacggggcg atagtgtcca
    45421 cttctttcca tactcgcttg gcgaccttcg ccgcctcgcg gtagatctca tccacatcga
    45481 gatgggcgaa ggctcgattc tccatcacga ccttcccatc gacgatgacg ctctcgacgc
    45541 agttgctgct catcccccag atgaagtgac tggcggcatt ctcctgaacg agcggggtcg
    45601 ggttgtggta atcggtgatg accagatcgg cgaccattcc cgcctcgacc cggccgaagg
    45661 agccgtcgaa gtatttctcc accaaccgat agccccggtt cagcgcctcg acgtactgcg
    45721 gcggccacca ggagagtcct tcgtccttgt gcttgaagaa tccgatcttc agctcctcga
    45781 acatattccc gccgcagccg tccgttccga tcagaaggtt cttcacatcc tgaatgtagg
    45841 agcagtagcc gacgtggttg ttcatgttcg atcgggggtt gtgggcgaag aacacccctc
    45901 gttcattgat cttcctgatc tcgtcctcat tgagatagag gccgtgaacc agaagggcgt
    45961 tatcagtcag aagcccatac tcgtcgagcc ggtcgacgat gtccatcccg taccggtggt
    46021 ggctgtgggc cacgtcgtac cgatcctcgg cgacgtggag gtggattccc accttcgtct
    46081 tctcgacaag gtcgcccatc agcttcaatc cctcattggg gatcgtgaag ggggcgtgtc
    46141 ccccgatcat cgccttgacg aggctgctct tcttcgagcg ctcgatgaag cggcggcttt
    46201 cctccacccc cgcctcgacc tcggccattc cgccgttgcg gtcggtgatt tcataacagg
    46261 tggatccgcg gatgccgacc ttctccatcc ccttggcgat cgtgtccaga gagccggcga
    46321 tataggaggg gctggcgtgg tggtcgatcg cggtggtcgt ccccgccatg atcgcatcga
    46381 gcgaacagat cagggagctg tagtagcacg cctcctcatc caatccgcgg tccagacgcc
    46441 accaccactc cttgagaacc tggatgaagt ccctttgggg tccggcctgg atcagcattc
    46501 cccgtgaaag tccgctgtaa tagtggtggt gcgaacagac gttgcccggc atcaccgtct
    46561 tatcggttcc gtcgatcacc ttcttggcgg caaccccctt ggcggcatcc ttgccgacct
    46621 tggtgatggt gtttccatcg atgacgatat cgacgccctc caaaacttgg aacgggggct
    46681 cggtctgcat gattcgggta ttcttgatta cgagatctgc catttcttac tcctcaacct
    46741 ctcccaggag gtagctgtag gaggcaaaca cctcctcgac gatcgcggcg acttcatcac
    46801 tgaggccttc ctcttcaccg acgagcactc cgtccttatc cagttcacac ggatagagcc
    46861 tgccgtcgac ccggatcagg atcgtaccgt cttcgacgag gaagccactg ttggtggagt
    46921 tctcaaagtc gtcccggcga ctgaagaggg tgaacttctt cttatagggt ccgccgtcat
    46981 acgggcagaa cgtttcacag tttccacact cgttgcagaa ggcatcgagg tgaagaatct
    47041 ggaaaggatt atcgaacaga ccggtattgc gaagatcaat cggaacgttc gcccggttgg
    47101 ggcagacgtc gacgcatttg ttgcagatgt aggagcattc catgcagcga tccgcttcgc
    47161 gtcgggcaaa cccttgctcc ccgaagagct tcgagaagag aatccgggat ttcctgagtt
    47221 ccagctctcc gaaatactca tcctcctccg ctaccagctc ttcgatgggc tgatcctctt
    47281 catcgtggat gtgctcttca tcctcatcga tctcaacccc ttccgcttcg aggatccgat
    47341 cgatcgcatc ctcgacggca tcgcgggcgc tggcgatgca gcggacgacc gtcgaaggtc
    47401 cgctctgtgc gtctccgatg acatagacgc cgtcgatcgc cgaagccttg ctcttcgcat
    47461 cggtgatcgc ccaccctttc tcgttgaggg ggacaccgta ccaggagaag atctccccgt
    47521 ccactttttc cccgatcgcg gtgatcatcg tgtccgcccg gatggtgaac gtttcatcgg
    47581 agggaatcgg ccgtcttcga ccgcttgcgt ccttttcacc gaggaccatc ttgcggacgg
    47641 tcagcatgtt cccttcaaac ccttcgggat tcgccagata gatgaactcg gccccttccg
    47701 cccgggcgtt ctcgaactcc tcatggtcgg cgggcatctc cgcccgggtt cggcggtaga
    47761 tgaccgagac atgctcgacg ccgggaatcc tcaaggccgc acgcgctccg tccatcgcgg
    47821 tgtttcctcc gcctacgatc acgacatgct tgccgagggt gcacttggag ggatcctttc
    47881 ggaattgacc gaggaaatcc agcgaggtga tcacccgggt ccgatcaccg actaaggtca
    47941 gttcattgtc gatctcgctt ccgatcgcat agaagatgtg ggtgaacccc tgctttttca
    48001 gggtttcgac ggtgagcttt ttggtatcca ccccgaagtg gaactcaact ccgtgggctt
    48061 tgatgaactc gacgtcgctt tcgatcgctt caacgggcag gcggaagccg ggaatgacgt
    48121 gccgcacgac tcctccggcg gattcctccc gctcatagac ggtggtatcg aatcccgccc
    48181 tggcgaggaa gtaggcggcg gacaggccgc cggggcccgc cccgatcacc gcggcgcggt
    48241 attcggtttt ttccgtcgcg ccctcccaga gttgcagata gtcggcaaag ccgcgctcga
    48301 cggcgatccg cttcatatcg cggatcttca ccgccccctc gtagtccatc cgggtgcagt
    48361 ggagctggca ctgatggtcg cagatgtggc tggtgatcgc aggaagcgcg ttcttctcgt
    48421 agatcaccga aagcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagat
    48541 gtggctggtg atcgcaggaa gcgcgttctt ctcgtagatc accgaaagcg cctccccgta
    48601 gcggccttcg ccgacgagct ggacatactc ggggatatcc tgatgaatcg cacaggcgtt
    48661 cacacagggg gcgacgtagc agtcaaccag cggaagctgt tcatgaatct taatcgaatc
    48721 ggttcctctg aactctttcg tcgttgccgt atagagtcca tcctggatat cggcaacgag
    48781 cttttccaac gcgggaacat cgatggtttc ccgcttccat ccgtcactgg attcaagaat
    48841 ctcgaccatc tgcttcaggc gggtgtatcc acccggcttg agcatatcgg tggcgagagt
    48901 gatcgggtgg attccgcact caaagagcgc cttgacgttc acggcggtag ctcccccgct
    48961 gtaggagatt ggaagcttgc cgtcaaactc cttggaaaga agcaagccga cattggtgga
    49021 caagggaaga agagcccgac cggacatata catctcttcc ccgggcagct gctcctggtc
    49081 gttcaccgaa ccgagggtgt tggtgagctt cacaccgaaa ccgaggcctt tctccttcgc
    49141 caggtccacg agccgatgca gcatcttgac cgcatcgggg tattggaggt cattgtcgaa
    49201 gctttcctgt ttcaaggtga tgtagtcata tcccagattg tccaggattc ttcggacgtt
    49261 ctcatatccc aacagggtcg gattaagctt tacaaatgtg ttgaggcgct tttccgtaag
    49321 catataggaa cagatcgctt caatctcggc cggcggacat ccgtgcatcg tcgaaagagt
    49381 aacggacgga gtcagattcg ggctaatctt gtcgatcgtg cgaagcagtc tttcctcttt
    49441 cccttcccag ggggtgcctt cgaagagtcc ttccttgagc agggtttcaa gctcgttccg
    49501 gtagcgggca aaccgctcat ccttgccggc gtcgatcatc gtgtcgatga acgcctgcat
    49561 ccggggagtc ttgatccctt ccaggttgta cccgacggac atgttgaaga tgaaggaagg
    49621 cttgccgaac tcccgatcgt tgagcgtcac atcgagcaca tgcaggatgt accacgcctt
    49681 gatgtattca tccgccgcct tgggcaaggt gaactcggtc gaccactcga cgttgtagcc
    49741 ttcatcccgg gcatcgatgc agggcttgtc cacctcgagg gtatccaaga tctgaacggt
    49801 cttcagttcg atgaagcggc cgccgaccaa atagcttgca acgatgttct gggctaattg
    49861 ggtgtggggc cctgcagcgg gaccaagggc ggtgctgcac gtctggccga agatgccgag
    49921 ctgcttctct cccttatcat gatagaagag atcttcactg atgctgaaga tagagccatg
    49981 attgcgatat tcattgacga ttctgtgtaa gagttcgaca aatggaacgg gtcgcataat
    50041 gtccgacata tctcactcct tttgggctgt agccaccttt cattttatag gagtttgccg
    50101 agaagtcaac gtaaaatgca acaaattgca acagttggat tttctcgccg actgtgattt
    50161 tattgaagaa gaaaaagggc tgtgataaaa ttgaattgga aggagggctg ttatgttctg
    50221 gatcaacaaa gaagatgaga aaaaaatggc tcttatcgct ttcctcattg tttctttccc
    50281 tgtttgggga acggttctaa tgattctact gtcttttttc atacgtagcc tgtaataatc
    50341 tttgccacat cctttttcca gagaaggaaa aaggatggct gattcccgtt ttaatatcaa
    50401 agtctcggca gatacgattg atgcttccaa gaagctttag aagctttgtc ttcccagcac
    50461 atacgggggg tgtttctgtt tttattcaag ctctagatgc agggaactgt attatttcca
    50521 ttctgtcatc aatagagcaa tgaataggac acatcaaagt ctcaaccgtg tgacaacagc
    50581 gggtttttct cgccaacttg tcaaatcgtt tccgatgaag tacgctatcc ctaccacgca
    50641 agaaggatcc ggtatgaaag aatatttgat tggatttgcc atcggtctca tcatcgcctt
    50701 agcggtgttc atctattacg tggtcaagcg cgcaaatgat caacgggcac atgcaaaaga
    50761 agtcgcccga ctgaagaaca tgctcagcga ccggatggac attgaaagtg aaggcctgag
    50821 aacgctgaag gaagaaaacg ccgaactgaa gaagcagaat gaaaaccttc ggatcaccct
    50881 caacaccctg agccagaaac ccgggcgcaa ggaggtcaac cgccttcagg tctatcagct
    50941 ggccgtcgat cggctgacga tcaacagccc cggcttcgga cctgcctggc aggcggcgct
    51001 gaaggaaagt gaagaggagt tccagaaaaa ccttacggga gcgacggcgt tcatcaagcg
    51061 gctgattccg gtgaagaccg aagcggcggt acttcccgaa acgatcgatt gacgctatca
    51121 tcgtgcctat gccgaaatgg tggaacaggt agacacaagg gacttaaaat cccttactct
    51181 tcggagtgta cgagttcaag tctcgttttc ggcaaactta aaacctttta aaatatagaa
    51241 ttgaaaaatg accaagaaaa ctcccttggt catttttttt ggttcaactc ctccaatttg
    51301 aaccaactct tgtcaaccaa atactccctt tgccctattc gttcttccaa atactcttct
    51361 ctatggagat ccgatcgaac gtgtagacgg ctccccctgc ctccttcggg gaacaggcgt
    51421 acataccggc gaggtaatga tccttgtggg catgtatatg gcaaatccgc atctgagtcc
    51481 agtcgttccc gtcgtacgag tattcgacaa gcagatcatc ccctttgctg gagactcgat
    51541 agtgcattga attgatcgat gcggggatgt cgaccgtcgc ccagtcgctg tagccgaggt
    51601 tcgtgcacac cgaacccagg cggctgatct cgtcgttctc cgcttcgatg ctgatcttga
    51661 tccagttctc ctcatcgatt cggacataga gtcccccttg gtcgtactga ttcttggctt
    51721 cgaaggtgca gtgagcagta agggtaaact catcccctat ccgggtaaga agcgcatgag
    51781 cgttgtccgc ccggaagccg tagtgggttc gttgccagaa atccgtcgat ccgcttgtcg
    51841 tcagcgttac cgaaccatcc tcaaacacgt gcttcggctc gttgctccag agaaacgggt
    51901 atttgtcgat aatcatcttc gtctcctccc tgtacagtct accatccacc ctgcacaatc
    51961 ggctatactc aggcaatgca taccctgttt atatacggat ccgacgcact tgaccatctg
    52021 aaacgaaacg attcccgtct gggtagggcg atcgatcgga tcggtatgat ccatcggacg
    52081 attgaaccgg atttcttcac cgcgatcacc cgatcgatca tcggccagca ggtctccacc
    52141 aaagcgcaaa ccacgatctt cgaacgctta ctcgatctgg taggaatcct tacacccgag
    52201 gcggttctcc ggctttccct tgaagagctc aaaagcgcag gaatgagctt tcgaaaggcg
    52261 gaatacattc aagggattgc acataaaatc gtatcaggaa aactgaatcc aaccttgttt
    52321 gcatcgatga gcgacagcga ggtcatcaag gagctggtct ccctgaaagg ggtcggcgtc
    52381 tggaccgccg agatgatcct catcttctgt ttgcagagac cggacgtcgt cagctacgga
    52441 gatctgggga ttcatcgggg aatgaagctc ctctacggtc tggaagagat ttccaaggaa
    52501 aggtttgagg aaatccgagc tcgatacacc ccctatggtt cagttgcgtc gctctatctt
    52561 tgggcgatcg cccaaggagt cttatctcca ctggactgaa cggataaact tcgataagct
    52621 ggacactgta ttcaaaattt gcataattat gcaaacagag gggttttgaa tgattgaaga
    52681 acagggtttg acgaaagaag aaatcaaagc ccggaagctc ttggaagaaa aggaagcgga
    52741 cagcaagctg cgaagctatc cgggggtttt cggtcatgcg gttacggtgc tgttcatcat
    52801 ctggtcgatc ttccagctct atgccacgac gatcggaaag attgatgcga tgcgcctcag
    52861 aacatggcac ctgctctttc tgctcatgtt cacgttcctt ttgtttccga cctataaaac
    52921 cgaaccgttg aagcggaagg ctcccccggt ctgggattgg ctcctcctcg gcctcacctt
    52981 cttctccgtc ggctatcttc tgctgtacta caccacggtc gccaagcggg gcgggtatct
    53041 gaacaagacc gatctggtcg tcgcgttcat tctccttgcg gtcatcttcg aagcggggcg
    53101 aagggcgtgc cggtccctga tgtatctggg attgattttc ctgctctaca acttcctcgg
    53161 ccgcttcatc cccggcgacc tggggcacgt gggcttcagc ttgaaacggg tcctcagcca
    53221 catggtctgg ggaagccagg gagtcttcgg ggtgggaatc ggggtcagcg ccacctatat
    53281 cttcctgttc gtcctgttcg gggcgtatct gaaatattcc ggcttcagcc aattcatcaa
    53341 cgatgtctct ctgaccctcg tcggccgatc cgccggcgga ccggccaagg tggcggtcat
    53401 cgccagcgcg ttgatgggga tgatcaacgg aagcgcgatc gccaacgtgg cgaccaccgg
    53461 gacgatcacg attccgatga tgaagaaaac cggctataag aaggagtttg ccgccgccgt
    53521 cgaagcggtg gcttccaccg gaggacagtt cactcccccg atcatgggag cggtgggatt
    53581 cgtcatggcg gagttcatgg gggtctcgta taccaaggtt ctggtcgccg ccgcgatccc
    53641 cgcctttctc tactacctca gccttctctt cgccgtccac ttcgaggcga aacggctggg
    53701 cctttccggc ctgagcaagg agaacatccc cgatgcactt gaggtcttca aaaagcaggg
    53761 acaccttctg attcccttgg tcgtccttct ggtgctcctg ggaatcggat acactcccct
    53821 ctttgccgcg gtcatctcga tcttcgccac cgtcggagcc tcgtggttga gaaaagagac
    53881 ccggatggat tggaggacga tcgtacaggc cacggtcgaa ggggcccgct cttccatcac
    53941 cgtggggatg agctgcgtca tcatcggagt catcatcggc acggtgagcc ttaccggcct
    54001 ggggctcagc ttcggcttcg tcatcctgag gatcgtggga gaaggccagc tcttcttagg
    54061 tggcctgatg gtgatgatca tgagcaccgt cctgggaatg ggagttcccg gcgtcgccgc
    54121 gtatgtgatc gtctccgcag tcagcgttcc cgtgctcatc cagaccgggg cgattccgat
    54181 ggcggcccat atgttctgtc tgatctatgc gtgtctttcc aatatcaccc cgccggttgc
    54241 gatgagcagc tacgtggcca gcggtatcgc cgattccaac cagaccaaaa caagtctgat
    54301 cgctgtgaag ctcggattga cgggatttat ccttcctttc tttttcctgg acaatccgat
    54361 tctgttgatc ggctcgactc cggatgttcc cattctcctg acgatccggg cggtcatcac
    54421 ctcttcgatc ggagtcgtcg ccctttcagc gggtctgcag gggtatctcc tgaggaatct
    54481 gacaatcatt gaacggggct tgctggtcat cagcggattg ttgttcatcg cgacgagtct
    54541 ctccaccgac ctcatcgcca caggcatctt gatcactctc ttgcttatcc aatattttca
    54601 aacacatcgt atgaaggagc gtatcgcatg aaaaaactca tcacaattct tttgatgatc
    54661 gccctggtcg tccccgtctt cgccgccggc ggcggtgaaa aagcccctgc aaagacgacg
    54721 atcaacttcc cgaccgcagc aaccaccggc gccgtgtacc cgctgggtgc ggcaatctcg
    54781 aacatgtgga acaacaaggt tcccaccgtc cgggctgccg ctcaggcttc cgccggcgga
    54841 atcgagaacc tcaacctgat cgctgacggc gaagcgcagg tcggtgtcgc cgtcacctcg
    54901 atcatgtacg agtcctttac gggaaccggc acctttgaag gtcgggccaa ccccaacctt
    54961 cgggtgatga tcggactcta ctacaacccg aaccaggtgg tcgtcgccaa gaactcgaac
    55021 atcaactccc tcaatgacct tgcaggcaaa tcctttgcca gcggtgctcc cggttcgact
    55081 accgaagtcg agacggcgat ccacctcaaa gaatcgggga tcaactaccc cgatgcgctg
    55141 aaagtccagt acatcggctt caccgaggcg atcgatctga tgcgcaacaa gcagatcgac
    55201 ggtgcttgga tcatggcggg aattccgaac gcggcggtaa ccgagatgct ctccaccgca
    55261 ggcggaaagc ttctccccct cgatccggcg ctcatcgatt ccctgcacgc caagcttccc
    55321 tggtatgcag cgtacacgat tccggccaac acctatcccg gccaggacga ggatgtcctg
    55381 accagcgcta tcaagctcac gatctgcacc gacgcccgcg tcagcgacga tgtgatctat
    55441 gaactgacca aggcgttctg ggaaaacatc gacgagctga aagccacgca ggcccccttg
    55501 aagaacacct tccttgagga gacggttcgc gacctcgccg gtcttccgat ccacgacgga
    55561 gccgcccgct actatcgtga acggggcctc ctctaacccc cctatcccgg gacttcggtt
    55621 ccgggatttt tcttcctttg atgacacaat gtgtgcatgg tcagggaatt cgggggtatt
    55681 tcgatattgt ttatatgatg gtatcatttg ccaacccatc agattgatct tctttgttgc
    55741 tgcatttcga ggcttaacgc gggtcaggag gaatctatgt cgtcatttat caaggattat
    55801 caaagaggaa atagaatata cgaatgggat ggacggtatc tgaaagccta tcaggatggc
    55861 caaaaaatga tcgaatggga cggccaatat gtcaaatcat actccaatgg cagaaggctt
    55921 tttgtatttg atggccgtta ttttaaaaga tattcggacg gacaaaaact ttatgagtgg
    55981 aacggccgat atataaccgc ttatcatgat ggcaggaaag tcatcgaatg ggatggacgt
    56041 tacatcaaat catataccaa tggaagaaag ctgctggaga ttgatgggct tatccctctt
    56101 gcgatcataa taggggtggc aacaggaatg atgtaatcat tgatgccgac aattcgacga
    56161 ttccgttttc aaatcttccg gaacgacttc tctgggattt cttttcccct tgactaagac
    56221 ccttccatca ctgatgctca gggtatggaa caagcgttta tcgatttgca tctctctttg
    56281 gaacttcagc ggaaggtgaa gcatctgtcc aatcggatcg gcgacgattc tcagcatcga
    56341 ctgctgatcg atgaattgag aagcgcgatc gtcgatcgtg atcagaaccg ggtggggtta
    56401 ctgctcaatc aggggctcac cgggttcggg gttctgcatc cccttgtcgc aatcgcccaa
    56461 gtatgggaaa cgatcggaga ggtattccat gcgcttggcc ttgatgatga aacccgtctt
    56521 gcaacactga atgacatcaa tctctggatc gcccaccacg ccgaagcgaa caacgggaag
    56581 cagggtcttt cccgggtgtt ctgggtcagc cggttgctga ctgcccgcat tgtccagttc
    56641 ggacgccttc aattcgagga gcgcgtcttt ccctaccctg cgaccgtgta tcaagatttc
    56701 gggcttgagt ttatcgcact gagcaacggc gggctgaccc tcgatcggga cggctatccc
    56761 accaaggatg cgaataagat tactcgaacc actgtcggtc gggaattaat cgatctatcc
    56821 agtggatcga tcggtgaaac gcgaatcgac cgttcattgt tgaccaaggt cttggatgag
    56881 caatcccggg tcctctttgt ccatattccc gcttcagggc cgcttgatcc tcatttggtg
    56941 gaagaagcgc tccggaaggc tgaagcattt tttggaagag gatgggtata tgtttgcacc
    57001 tcctggctgc tcgatcccgc ccttgagaag gtagtggatt ccgaaagcaa catcctgtca
    57061 ttcaaccggc gctttttgaa gctgcccgtc ttcttcgaat atccccagct cttcgagcgg
    57121 gtcttcttct gcaatgacga agcatcggtt ctgtccgctg atgcgacgac aagtctgcag
    57181 aagaacgtgc aatcagcgct tcggcgcgga gtcgtttttc gaacgatggg cggattcttc
    57241 cttgcataaa cgggtgggag ctgatactac acaggtatgg aaacaccgct gcacggtctt
    57301 gtcgactccc actttcatct ccttgcgatc cgagagaaag gaatcgatcc ttccccgttg
    57361 ctcgccacga tgaaggagct cgggtatcgg ggaatcgata tcggcctcgg cggaaatgac
    57421 cttgccgagc ggatcgggct ggtcggcggg tataaagaga tctatttcgc caccggtctc
    57481 ggtccgtggg cggcggcctg ggaggattgg agggtcaagg aagcgcttca gcttctggtc
    57541 tccgatcttg ctcggtatcc cgtgctcgcg gtgggagaga tcggactcga caactactgg
    57601 aagtacggaa ccacagagcg tcaggaggag ctcttcctcg cccaagcgga tctggggaaa
    57661 ccgatcatca tccacaaccg cgaagcggat gaacagttca tcagactgct tgaagaacat
    57721 tcctttcctt ttgggggaat cttccactgc ttcagcggat cggaagagct tgccgacctt
    57781 gcgttggaaa aggggtttta cctctccttt gccggaagtc tcacctacaa gggtagcgag
    57841 ccgctcatcg ccctgctgaa gcgaataccg aaagaacgcc ttctgttgga aaccgacagc
    57901 ccgtacctcg ccccggttcc caaacgggga agaaagaaca ttcccctcta catcgaatat
    57961 ctctatcgct ttgtcgcaaa caagttggaa cttccgattg aaaccctgat ccggcttgta
    58021 tcctccaatt tccaacgctt tgtcgatgag gccaaggttg accgtcctta agcgattcga
    58081 tacgataatt gttgactgaa tcagatcaga taaggagaga ttttatgata tcctacaagg
    58141 acctcggatt agtgaattcc aaggaaatgt tcgccaaggc gattaaaggc ggatacgctg
    58201 tcccggcgta taacttcaac aacctggaac agctgcaagc gatcatccaa gcatgtgtgg
    58261 aaacaaagag ccctgtcatt atgcaggttt ccaaaggtgc ccgcgactat gcgaacatca
    58321 acttattgcg caacatggcc cgcggtgccg gcgagtacgc ccgcgaactc ggctatgata
    58381 tcccgatcgt cctgcacttg gatcatggcg acagctttga aacatgtaaa gattgcatcg
    58441 acaacggatt ctcctcggtc atgatcgacg gctcccacct tccctacgaa gaaaacgttg
    58501 cgctgacgaa aaaggtcgtc gaatacgccc acaagcacga tgtaaccgtc gaaggtgagc
    58561 ttggcgttct tgccggaatc gaagatgagg tttcgcacga gaaatccacc tacaccaaac
    58621 cggaacaggt cgtcgacttc gtctcccgga ccggcgtcga ttcgctggcg atctccattg
    58681 gaacgagcca cggagcgaac aaattcaacc ccgcccactg caccgttaac gaagaaggca
    58741 ttctcattcc tcccccgctt cgcttcgata tcctggaaga gattgaaaga cggcttcccg
    58801 ggttcccgat cgttctgcac ggatcttcct ccgtcccgca ggagtacgtg aagataatca
    58861 acaaatacgg tggaaaactg aaagacacga tcggaatccc cgaggaacag cttcgcagag
    58921 ccgccaagag cgcagtgtgc aaggtcaaca tcgacagcga tggccgcttg gcgatgacgg
    58981 cgacgattcg tgaaatcctg cacaacaacc cgggggaatt cgatccgcga aaatacctcg
    59041 gaccggcccg cgacgaactg aaaaagatgt atatccacaa gaacaccaac gttcttggtt
    59101 ccgccaatca agcctaacgt aatcgatggc cgccttcggg cggctatctt cttatattcc
    59161 agctttgaca catcgtaaga ggctatggta tagtaatttc ggtcacccat acgggctgaa
    59221 gtatattact gcagagtggt tttaacacct aaatcacaag gagaacattt ggctacaaga
    59281 gatttgaggg tcaataggca gattcgtgcc agagaagtac ttgtcattga tgaggatggc
    59341 aatcaaaagg gcattatgag cagttttgat gcaatcgagc tcgccgaaca agcagggctc
    59401 gatttagttg aggtttcccc caacgccaac ccccctgtat gcaaaattct cgattatggt
    59461 aagttccgct acgagcagga aaaacgattg cgggatcaaa agcggaatca ggtgatcacc
    59521 aagatgaaag agattcggat gcagccgaag atcgaacagc acgacttgaa caccaagagc
    59581 aaattcatcg gagacttcct caccgaaggt aacaaagtaa aggtaagtat tcgcttcagg
    59641 ggccgtgaat tggctcatac cgaattgggc aaagtggtcc tcgaccggat acttgcccag
    59701 ctcaccgata atggtgtagg atacgtcgtt gaccgaccgg caatgatgga aggacggatg
    59761 atgagcatga ttctctcgcc cgccaagacg tcggggccga aaaaagaacc acagtaacca
    59821 gtatcccgca tgggatagtg aatacgttgc tccaggggta tcttgttccc tgtggcacag
    59881 aaagaggtgc tatcatgccc aagatgaaaa caagaaagtc cgctgccaag cggttcaagg
    59941 tgacagccgg aggaaaagtc ctgtacaaga aacagggcct tcgccacatc ttgaccaaga
    60001 agagcagtaa gcgcaaggcc cacctgcgct caacaggtgt cctgtccgat gtggaagcac
    60061 gacgagtcaa gtcgatgctc ccctacgagt aaggaggatc gggatgccaa gagcaataga
    60121 tggaaccaag cacaaggaca gacgtaagaa gatcctcaag ctcgctaaag gccatcgcgg
    60181 ccggcgaagc acgaactaca gggtcgcaaa ggacagcgta gagaaagccg gacagcacgc
    60241 ctatgtcgga cgaaagctcc gaaagcgtga tttccgccgt ctctggattg tcaggataaa
    60301 tgcagccgtg caggccgagg ggttgaatta ctcccaattc atgcacggga tgaaactcgc
    60361 caacatcgaa gttaaccgca aagctctatc caacatggct atcgaggata aggccgcgtt
    60421 taaaactctc gttgaccaag taaaggtcgc ccttgcaaaa taagggcgca ggaggaagca
    60481 tgtctgtcat agataccgta cgactcttag agcttagaac gaaaaaggcc gccggcctca
    60541 tcgccttgtt acggcgggaa aaagaagaac tgcagcagcg gttcgacctg gttcttgccc
    60601 acaaccggga attggagggg tatatcgaag catataccac cagtgaaaag ctcgtcgagc
    60661 aatccatcgc caccgcgatg gccaccctcg atacgatcga cggcttggat gagattcaag
    60721 tctttgacga tgcttccctt gagcttgaag ccgccgacac ctttacggga gccgatggcg
    60781 gcctcagcag cgaggatgtt gattgggatg cgcttctgga tgatcttccg gaactgtaac
    60841 ttcctgtctg aacagcgtct atgggacctt cgggtcccat tttttgtccc tctttcccaa
    60901 aaccggttag tgtagtacca tgttgatagg tggatgccac ctgacgatta ttgtaagtat
    60961 tcctgcggtg ttctcctgga gacaaagtct cttggctcaa ctaacacaac cgggacgcgg
    61021 tatagctgtt ttgcattgat ccaactcctt cggggtttaa cggaacagaa ttttcagccc
    61081 aactggtgtc ccccttgaac cacaggttca agagcgttct tcggttgcgg caccgcagga
    61141 ttttctatgg agcaatgtca ccatgcatga agcgctgaac catgtgatct tcatctccct
    61201 ccccccctcg atggaaagga agatcggatc attcacgatc gacccggcga tccggcttcc
    61261 cgtcgccctg gccgatgacc atacagcaat cgatgaacgg tcgatcagca tcgaacgaat
    61321 cgtcagcggg atgctcaaag tccttgcgta tgacattgat catcaacacg ccccgtatta
    61381 tcgagaattc gtgctggcgg ctcagcccga tgcggtaaaa gaactgacga tagccgcaat
    61441 cgcccagcaa caaaagggaa acctcacctt cgccgaagag ctctttctca ccgtctgtca
    61501 cctcagcccc caatccgcta cctatatcaa tctggccacg ctgtacggac atcgggcgac
    61561 cgaggattca agcaagggtg aaacgtatga cttctaccag caaaaagcgt tgaacaccct
    61621 tgtcgagggg ttgtccgttc tgggtgatga cgaagcactt ctgaaagaga tcggcttttt
    61681 tcatctctat caggggaatg tcgagatcgc ccgggactat ctggttcgat acgtcgatct
    61741 tgccggggaa tcgaaagaaa aacggcacac gatgaagatc ctgaaggata tcgaaacaaa
    61801 gcttgccggc gaacaacgcc tcatgcaggc ctacgatgcg atccacatga acagggagga
    61861 ggaagcgctc tccttgctga accggtatat cgaagcatat ccggaggtct ggaacgccta
    61921 tttcctgaag ggttgggcgc tccggcggct tgaacgcttc caggaagccc aagacgccct
    61981 catccaagcc ctggagctgg aaagccacag cagcgacatc tacaacgagc tggccctgtg
    62041 cgccttggaa gcgggcaacg aggagctggc aaaaagctac ctgggaaccg cccttgatct
    62101 cgatgaagag aacataacgt tcatgagcaa ccttgcgtac ctgcaccttc ggagcgggga
    62161 actggaagag gctcgccatc tgcttgaaca agcccgctcc ctcgacggcg aagaccccct
    62221 catccaacga ctgatggagg actacaccca ccaaagcggt gacacgctgg gagatgtcat
    62281 catcgaagag tacttggaga acgaagagat cgccgacaag ctgaaacatg gagccaagga
    62341 gatatgatcg ttcggacaac gggggaaaag gagacgatcc gggtcggata tgctcttgga
    62401 aaaacccttg cgaagggcgc ggtcgtctct ctgcggggat cgctcggcgt agggaagacg
    62461 gtctttgcca ggggaatcgc tcagagcctc ggaatccggg aagcgatcgt cagccccacc
    62521 ttcaccctga tccaggagta cgaaggaacc ctccccctct atcacatgga tctctaccgg
    62581 atcgacagcg tggaggagtt tgagctgatc gggggggagg agtatctcta cggtgagggg
    62641 atcacgttga tcgaatggag tgaaaagatc caagagcttc ttcccgaaga gacgatctcc
    62701 gttacgatca ccctcgccga ggacggctcg cgggagatcc gaattgaagg aagcatgcta
    62761 tgattattct cggttgcgac acctctacaa aaacggccaa cctctttctc ggcgaatatg
    62821 aggaggatca actctcgtgg tatgagacaa cctcggtggt ctggaacaac acccacagcg
    62881 aacaactgct ggatcggctg cttttgatgt gctccgatca ttccatcgaa ctttccgaat
    62941 tggacctcct ctgcgcaagc agcggtccgg gatccttcac cggcttgagg atcgcccttg
    63001 ccacgatgaa ggggctcgcc ctcggattga acaaaccgct ggtcagcgtc cccacgctgg
    63061 atctctttca agggatcgcc cgattcatcg gcggagcatg ccttgcggtc atcgatgcaa
    63121 aaaaacagcg cttttacacc gcgctgttcg tggatggaat caaaatcgga gaaagcagcg
    63181 atctgagtcc ggatgaaatc gcttcgctta tcgcccccca cccctccatt accctgcttg
    63241 gcagcgacgg aactctcctt gcgaagcgac tggcagatcc gaaggtcgca gtgtttgagg
    63301 ctcaccgtca gaatctcgga gtggagctag ccgacagggc aagaaagcat tatcttgaga
    63361 caggcgccga tcctttggat caggggccca catatattcg taaaagcgat gctgaacttg
    63421 cattgaagga gtaagaacat ggttgagcag gagattttgg tcctcggttc gggtgtggct
    63481 ggcatggcgg ccggccagta tgccgcccgg gcgggcagaa cggtcaccat tatggaatcg
    63541 atcgcccccg gtggacagac gatgtatata gatctgattg aaaactatcc gggaatagag
    63601 gttcccgtaa gtggagcgga gatcgcgctg aagttccaaa gtcaagcgga gaccttcgga
    63661 gcgaagatcg tctatgaaac cgccacgtca atcaggaagg aaggtgatga cttcctcgtg
    63721 gagaccgcaa gcggcaccac ctacaaagcc aaagccgtca tctatgcaac cggagcgaaa
    63781 caccgccatc tggaaattga aggtgaagaa gcctacgccg gcaagggcgt ttcctactgc
    63841 ggtacgtgcg acggtccctt tttccgaggc aagcggattc tcgtcgtcgg cggaggcgat
    63901 accgccatca ccgatgcgat ctacctttcc aagctgaccg accatatcac catcgcccac
    63961 cgcaaagacc gattccgggc gcaggacaac ctggtcaagc agatcgagcg caacccgcac
    64021 gtcgagttcc tgatggagca cacgcttcaa gagataaaag gcgacggaaa taaagtcacc
    64081 tcggtcatcc tcaaggattt gaagcaggat cgcgaatatg agcgggaatt cgacgccgtc
    64141 ttcatctttg tgggcatcat ccccgagacg aaccttctca agggttctta cgatgtcctc
    64201 accgaggatg gctgggtcat taccaacgaa cggatggaaa catccctcga agggctcttt
    64261 gccgccggag acgttcgtga caccgtcttc cgccagttga tcaccgctgc gagcgacgga
    64321 gcggttgctg cacactgcgc aagctcgtac atcgatgaat tggaaggcag agcatatacg
    64381 taaaacccaa tttccttgac gcaagaacgg ttaaggtgtt ctaatgggac tatgaagata
    64441 tgcatggtct ccagtgaatc ggttcccttc tccaaaagcg gagggttggc ggatgttgtc
    64501 ggcgccctat caaaagctct ggcatctctt ggtgaagaag tacttgtcgt attgccccac
    64561 tatgggttca tgaccgatga gttgattcca accgatatcg agcataccgt ctctttgttg
    64621 gggagtgaag aacgggtcac atatgcaaag ctttcagaac cgggtgttga atactacttt
    64681 gtccaacacc cgttttttac tgaatcgaaa ggcatctatg ggccgacctc ctcatcctcc
    64741 tacgaacaca accatcttcg atacacgctg caggcaaaag cgtctttgga gctcttgaag
    64801 atccttgggt ggaaaccgga tatcatccac gcccatgatt ggaccgcagg cttcctgccc
    64861 tacctcgtga agcgggattc atccggcttc ttcgagaaaa ccaagacggt cttctcgatt
    64921 cacaacctcg cctatcaagg cgaattcagc cgtctcgatc tccttgaaac ggatatcgag
    64981 gtcgatcccc gtttctttgt cggcagcggt ccgaaaaaac gagccaatat gctgaaaacc
    65041 gggttggagt tcgccgatac cctttccacc gtcagcccaa cctatgccaa agagatccaa
    65101 actcccgagt acggctgtgg actggagggg ttgctccagc gccggaagga cgatctcgtg
    65161 ggaatcatca acggcataga cattgacgag tggaatcccg aaaacgatcg actgctcgac
    65221 tactccttct ccatcgatga tctgtcaaac aaagctcgcc tcaaacggca aattcaggaa
    65281 gagttcaacc ttgaggtcaa tgccggtatt ccgctgtttt cgatgatcag ccgtttggtg
    65341 agtcaaaagg gatttgtcga attgctggaa ggagaggtct cggcgttgga ggaactgctc
    65401 acaacagaag agattcaagt cgtcgtcctc ggtacgggcg attgggagct tgaaaaaaag
    65461 ttcaccgagc ttggagcgag gttcaagaac ctttcggtga atatcctgtt ctcaaaccgg
    65521 gccgctcacc ttctcgaagc cggaagcgac ttctttctca tgcccagccg gtatgagccg
    65581 tgcggactga accagtttta cagtcttcga tacgggacgc ttcccgtagc cagaagaacc
    65641 ggaggactgg cggattcgat tacagatttg gatgaagatc ctcaaagagg aaccggaatc
    65701 ctctttgact caatgaccgg tcgtggtata ttggaggcag taaggagagc tcttacttgg
    65761 tggaaaaaag gaaggacggt cattgaacca atccgaaaac gggcgatggc ctacgacagt
    65821 tcgtggaccc gctcggcccg ttcctaccaa gagctctatc gcaaaacaca ggggggtttt
    65881 accaaatgaa tcgtcgaaag ccaaatgtca ttgctatcgt actgggcgga ggaaagggga
    65941 ctcgtcttta tcccttgaca atggatcggg caaagcccgc agttccattt ggcggaaagt
    66001 atcgcctcgt ggatattccg atttcaaact gcatcaacag cgggatcaag aaaatttata
    66061 ttcttactca gttcaactcg gcgtcgctgc acaaccacat ctcaaacacc tatatcttcg
    66121 ataccttctc caaagggttc gtcgagattc tcgccgcgga gcaaacgata aaaaccgaga
    66181 cctggtatga aggaaccgcc gacgctgtgc gaaagaacct gcgccacttc cgcgatcagg
    66241 aagcggatta cttcatgatt ctcagcgggg atcagctgta tcgaatggat ctgatggata
    66301 tgctgaaccg gcacatcgat tccaaagcgg agtttaccat cgccgccaaa ccgatcagcc
    66361 gcgctcaggc gaccggcttg gggatcatcg gctgcgacga caaggggatt attaccagct
    66421 tcatcgaaaa gccagctccc gatgtggata tcacttccta taaggtgggc ccaaagttca
    66481 tgaaggaatc gctcggagtt tcagtcaccc gtgaaaacga gtatctggct tcgatgggaa
    66541 tctacatctt caacagcaag acgatggaag aggtcctgga caataagaag accgatttcg
    66601 gaaaagagat cattccccaa gagatttcaa agcgccgcgc cgccgcctac ctctttgacg
    66661 gcttctggga agatatcgga acggtcaagg cgttctatga aacaaacctc gaccttgctt
    66721 caatcaaccc gatgttcaac ttctatgatg aggagatgcc gatctacacc caccgccgac
    66781 acctgccggc aacgaagatc aacttctgca acatctccaa ctcgctgacc agtgaaggct
    66841 cgatcatcac caatgcgtat atcgtgaact cgatcatcgg tgttcgaacc cttatcgaat
    66901 cgggagcttc cctcgacgga gtgtattgca tgggcgcctc ccgctacgaa acggatgagg
    66961 agaaagaaca gaacgcaaag aagggcattc cgaatatcgg catcggccgg ggtacgatca
    67021 ttcgcaaggc gatcatcgac cagaatgccc ggatcggtga cggatgccga atcggcattg
    67081 atgatattcc ccgtcaggaa ggagacttcc cgatgtattc catccacgac ggaatcatcg
    67141 tcatcaataa aaacgcagtc attaaaaatg gaacggtgat gtaggaatgg atgaacgaaa
    67201 gagacagatt gaacgtggag aaacaatttt aggcattgaa ctcggatcaa cacgaatcaa
    67261 ggcggtcctc atcgactcgc acaacgagcc gatcgctcaa ggcggtcaca cgtgggagaa
    67321 ccagctcgtc gacggagtgt ggacctacag cttggaggcg gtcgaagagg gcttgcaggc
    67381 gtcattcaaa tcactgaatg atgatatcaa agcccaatac aatgtggtgt tgaagcgaac
    67441 gaaagctctg gcgatcagtg cgatgatgca cggctatctg gcgttcgaca acgaggacaa
    67501 ccttcttgtt cccttccgaa cgtggcggaa cacgatgacc gaacaagcgg cggaggatct
    67561 ctccaagctc ttcgattatc cggttccgga gcgttggtcg atcagtcatc tctatcaggc
    67621 aatcctgaac aaggaagagc acgttccaag aatcgccaag atcacaacct tggcgggata
    67681 cgttcattat cgactgaccg ggaaacacct cttgggaatc ggagacgctt cggggatgtt
    67741 cccggtcgat ccgaagacca aggggtatga ccggacgatg gtgaagcagt ttgcttcatt
    67801 gcacacccat ccttggaggt tggaagagct cttgcctgct ccgctttcag cgggagagga
    67861 ggcgggagtc ctcaccaaag agggggctct cttccttgat ccgaccggga ccttggaagc
    67921 aggcatccct ctggccccgc ccgaagggga tgcgggaacg gggatggtcg cgacgaacag
    67981 cgtggccaag cgaaccggga atgtgtccgc aggaacgagc gtctttgcga tgatcgtgct
    68041 cgagaaggag ctgtcccgtt cgtataacaa attcatcgac ctcgtcacca cccccgacgg
    68101 ctcgctcgtt gctatgagcc acgccaacaa ctgcaccggt gaatacgatg cgtggatcgc
    68161 tctgtttgat gaagtgctcc acgccgtggg gcacagcgta cccaaggcga agctgtatga
    68221 cgatctgctc ggcaaagctc ttgaagggga ttacgacggc ggcggactgc tggcgtacaa
    68281 ctacctctcg ggagaaacga tgaccgagct gtatgaaggc agacccctct tcgttcgaaa
    68341 ggcggagagc tccttcaccc tcgccaactt catgagaacc caactctaca ccgccttggg
    68401 cgtcctgagg atcggaatgg acatcctctt tgaacaagag ggggtgagca tcgattcgat
    68461 caccggtcat ggcggcttct tcaagaccgc attggttgga cagaagatga tggccagcgc
    68521 tctgcacact ccggtggcca cacttaaaag cgccggtgaa ggcggagcgt ggggaatcgc
    68581 gttgcttgca tcctatctcg tcaataaaaa aggccgaacg ctcgccgagt tcctcgatca
    68641 ggatgtgttt gccggtgcgg agcgaacgat cgtcgaggcg accggcgcgg agattgaagg
    68701 attcaacaaa ttcatggagc gctataagga aggtcttggg atcgaacgag ccgctgtcga
    68761 gtttttgcgt taacgcaact gtgagataat cgcctggtca ctcggaggaa aggtgtacgc
    68821 atccaactcc gagaaggaaa cccaggcgat ttcagtatgc acactcagag tgattggttt
    68881 tgagtccacc cgcttcacct cataggcgcc caagtggtat cggacatggt tgttttcaaa
    68941 ggtgtgggtg gccagaagct ttcccacgac gacggagatg cccagctcct cttcaaactc
    69001 ccgaaccagg gcttcctccg gcgtttcgca atgacggcat tttcccccga caaactccca
    69061 tagacctccc accggtccgc cttcgacccg ttttgcaatg aggagcttcc catcctgtct
    69121 gacgatgcct gccgtggtat ttcgctcttc catcctgcat ctcacaataa cacgaaatgg
    69181 ggaaggagaa aatgaataag tgctatcacc aaggtgacat atcccatcct ctcccggctg
    69241 cgctcccgat accggcccac cacgctcgta aagagctcgt cggcttcaga tttttgaaga
    69301 gattcggtaa ggtaccagat gctcagtatg aagatattga tcagtggcag aaggtctccg
    69361 atgattcgag gtcccggatc gatgggaaag agggcaagcc cgatggtcag cagaaagccg
    69421 ccgatgatca gacccaggcg aaccgcccgg ttgttccgaa aggtgaaccg cagagaaaac
    69481 aggatgctca ttttgattcc gtgggtatcg gaaagcagga accccgagcc gatgacgagg
    69541 tacgccaccg aaagaagata cagctgaatc atcgctccgc ctcctttatg gcaagaccga
    69601 tgtacggaag cagggtttca ctgtcataat acccgatccg ctcattcgcc gtcagggcgc
    69661 tcttaaagag aatcttgccg gcgatgagac tcgaatcatg ggcgctgaac ccttgcccga
    69721 ggaaggcggt gatcatcccg ccgagcagat ccccgcttcc ggcaaccccc agcgaaggat
    69781 tgagcccctc taggatcgtg atcgttccgt cgggatcgac gatgtggatc aggctgcttt
    69841 tcagcacgat cgtcgcgtca agggtttgag caatagcttt cagctgactg aagaacgatt
    69901 cagggctctc gcttgacaag aaacagggcc taccgagcac cgcctccccg atgacccgca
    69961 tctctccgag gtggggggtg agaatcagcg gagcgttcct gctttcaacc gaatattcct
    70021 tcgaagcaat gagacgggca agcgaacgga ttccgtcggc gtcgatgacg agcggagtcg
    70081 acatggacag gagaaccttc agaatctctt cccgtccctc cccccacccc ggccccgcca
    70141 ggatcgcatc aaagccttcc accggttccc cccggtagga acgaaccatg acgctgaggg
    70201 tattcgatgc gcagatctcg tggatctcgg gatcgcagaa cagggtcacc aggccgcttc
    70261 gagcggtgaa cgccgcgctg gcagccagac gggccgcccc ggtgtatttc tcgcttgaac
    70321 cgaagattgc gatgcttccc cgggtgttct tgtaagcgga gcgattgaga gccggtatcg
    70381 caggcggctc gtcatcgacc gtggcgatga cgttcagcga gtcgaggagc gccaagggaa
    70441 acgagggatt ggcaatccgg aggttcccac agcggctgcg ggcaagagga tggtaatggg
    70501 cttgggtgaa cgagccgaga gccaccgtat gatcgctgga gatgcaaagg ttcgatacgg
    70561 cgcatcgatc gcccagaccg gaggggatgt cgacggaaaa caccgaagca tcggaatgat
    70621 tgacccatga aatgagatcg tggagcttct cattcaacgg gccgttcaag ccgaggccgg
    70681 tgatcgcatc gatgatccag tcacgggagg gaagatcgtc aaggtcaatg tcgcccagcc
    70741 aaagctgcct gatcggatac tccttgagga gcgtttgctg aaaagcgtgt tcatcagtgt
    70801 accgggtggc ggtatgaagc accgtgatct tccgccaccc ggcgtggtac gcatgacggg
    70861 cgatcgccag cccgtcagct ccgttgtttc ccccgccgat gagaaagagc agcgacgatg
    70921 accgctcaat gagtcgagac agtgcgaacc agcactgaag ggcggcgctt tccatcaaca
    70981 ccagcgatgg aagaaccttc ttggcagcgc tgtccaccct cttcaactct tcggaactcg
    71041 tgacgatctt cataccgata ctgtacagaa gagagagcat gttttcaacg tttccattct
    71101 tgtgcttcaa gcgcaaggct ttctacattc ttctaaggct tgccttgaat tttaccagat
    71161 tggttagtag tattgagtga ggagggttga gatgaccaag aagatagagt ttaagtatat
    71221 ttttgagaaa gactacaacc ctaaatatgt aaacggagct tttggtggaa tgtctcctac
    71281 gggggaaatg gtagttaatt tttatttgga gcgtatgcca ttgccctatt cagatttcca
    71341 tgatgttaaa gaaggacagg ttggggatct cactggcaga gagccggaag actatgattc
    71401 aaccatcgtc agatatatcc aaaacggaat cgtactctct ttggaacacg ctaaagcaat
    71461 tcataattgg cttggtttgc acattgccga agctgaaaga attgaagcac aaaaaaagct
    71521 cccccgttga agggagtaag ttatgcatag tgaattcaca tcatcgtcaa ccgcttccaa
    71581 tatcgtcact gccccggagg caaaaacctt ttctcgaaga ggattgcttg aagatactaa
    71641 tagtttgtca tctcattacc gattagctga tatcagagta gtggattttc ttaaaaatgc
    71701 tagtacctca tgttttagtc attcattaaa taattctcaa ataaagagat attttgaaaa
    71761 tactggtact tttgctatgc aaatgaacag ggttgaaaaa catttacaat cgttcaaaga
    71821 tttgaaaaca ggttggaacg ggtacgatgc accacctatt cctgaagctg taataagaat
    71881 agccggggat attctacgtc atataagata tgttgtgcct gaaatttttc ctaccggaag
    71941 agaatcggtt cagtttgaat ttgaccacaa cgatcattcg cttgaggttg aagtatttaa
    72001 agatgcgata catgttgact tattcaatga tacagatcta atcaaggaat ggtctcttga
    72061 tctcgatggt ttggaaggta ttaatgaatg catagcggag ttgtatggat actaagaagg
    72121 taggggaaga ggaggtttta tataggggta ttacgacaaa cccaacaatg tggaaatcct
    72181 cggaaaatcg accttcttcc gccgcattca agcagacgca ttccctctcc gttgatagag
    72241 aaggcggtcg ctcttttgtt gagattaaag aaaaccttat aaagaatatt tcctctaacc
    72301 ttaaagcagt agcctatttt tccgctaaag attgttttta taaccatatg actgttcgcc
    72361 cggatcctgt gattaataac ccatatcatg cccttattga tgacgaggga ggcgttggtg
    72421 tgggcaaga
//
LOCUS       DAOSLI010000003        64840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_524_length_64840_cov_31.022490, whole genome shotgun sequence.
ACCESSION   DAOSLI010000003 DAOSLI010000000
VERSION     DAOSLI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 64840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64840
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_524_length_64840_cov_31.022490"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1461
                     /locus_tag="PLF67_00975"
     CDS             388..1461
                     /locus_tag="PLF67_00975"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HOE88615.1"
                     /translation="MKKFLMIVRNIVIITIAMTILIASIRKINDIRYRPKGYDPSKAY
                     NAKDLSRYETDIDGVQVSRIIGDYMNGFRLLPEHKTHKGVLVTFGGSEGSPSYEFAEL
                     FAKEGYEVLALFFFGMDNQQPELVNVPLDFFKEVLAYIDENVDPDSDEKTPVTVYGAS
                     KGAELALNLAVIYPEIDNIVIMAPGAWSFMGLPKQYSQKMYASWTLDGKEVPYIDMSK
                     GDLKTVANLFLDFLLNRPIDYRPGYESASMNDPNSELARIKVENTKANILIFAGDDDK
                     MWHSEVSAKIIQKHRPKHTEVHIFEGAGHIFALGKYIYSPGMILEMGGSAEANKKAAE
                     ASYKIFMENMAEWHGKQAGDGMD"
     gene            1919..3103
                     /gene="nagA"
                     /locus_tag="PLF67_00980"
     CDS             1919..3103
                     /gene="nagA"
                     /locus_tag="PLF67_00980"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOE88616.1"
                     /translation="MSQRTVLINGTVVTGYGKLKDCGVYINENGKIGDIFNMSRFNNK
                     HFPPDTTILDAAGGYITPGMIDSHLHGIGGFGTEDSDPHSILQMSERLADVGVSAFMP
                     TVYTNTLDEMIRSIKAISDAMGQEKGAKILGINVEGPFISMKRIGAQNPEGVLPVDLA
                     IFNKIIDAGEGNIICMTVAPELKGMRELALLARERGIVLLAGHTDASYENILEGMQVG
                     ILHSTHFFNAMSRLHHRDPGTVGAILIEKDMQCEIIPDGVHVHPQLVKMLLRDKPLDN
                     IVMITDSLKPTRQRGGNLTANGVPATLGPNGAFVAKDDPDLFIGSGLTMLQGVRNVID
                     WEIPIEHAIQMSSTNPARIYGLSKMGMLIPGNIADILVLDKNLQMKGLFIDGNLIRDR
                     FA"
     gene            3126..4562
                     /locus_tag="PLF67_00985"
     CDS             3126..4562
                     /locus_tag="PLF67_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOE88617.1"
                     /translation="MQDAVRGLEPSALWSYFSDLSAIPRESGNEEGVRQYLLLFAEKH
                     NLTAIVEEVGNVIIKKPAAKGFEDRPSVALQGHMDMVCVKTEGSTHDFATDPIRLVRD
                     GDWIKAKDTSLGADNGIAVALILDILADADAKHGPLEGIFTVAEETGLTGAFGIEEKN
                     IDSRLLINLDSEEEGVFYIGCAGGVGTNAYMDVLWEETPSHHRGFELSVSGLKGGHSG
                     AEIHTQRANAIKLAARILTRLDEYRVFKATGGSKRNVIPSTATIGFSIPKEDEDRLYQ
                     VVETVLKEVKGEFEMADPHIAIDVTKVETPKRSVVANTLFDALYAAPHGVDAMSMSIE
                     GIVETSTNLAILSLDEKGFYVSASHRSSILSARDDVARRFATIFKLAGATTKHSGAYP
                     SWTPNIHSPLAALVARAWKEYTNEDAKVTAIHAGLECGIINSRVSGMDSVSFGPNMEE
                     VHSTKERVSISSTGRISDFTRYLLETIR"
     gene            complement(4566..5780)
                     /locus_tag="PLF67_00990"
     CDS             complement(4566..5780)
                     /locus_tag="PLF67_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOE88618.1"
                     /translation="MKPDLLTDHVYRVAAKIGNRDLFEGIWPIPDGVMLNSYVVKGSK
                     STALIDVVKDWDGALDVINRQLDELSVTTNTLDFLIINHMEPDHTGALREVVKRYPDV
                     QILCSPKAVPLIKAFYKIEENVQAVADGQTLDLGGKTLVFHLTPNIHWPETMMTYLQE
                     DEILFSCDAFGSFGRYDFCFDDELDEATMAHLHSETERYYANIVSSFSPFVLRGIEKL
                     KGLKIAMVAPSHGVVWRSHPEAIINHYIKLASYLNGPREKEVALVWSSMYGNTQALLE
                     SIIAGVESEGLPIHVLQVPQTHESFVLEKVWRSEGLIVGMPTYEYKMFPPTYHVLDIL
                     ARSHVKGRKTLRFGSYGWSGGAQKQYDEFVTAMKLECVAEAIEFQGSPTQEDKDKAFA
                     RAKELARIIKAM"
     gene            complement(5834..6529)
                     /locus_tag="PLF67_00995"
     CDS             complement(5834..6529)
                     /locus_tag="PLF67_00995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOE88619.1"
                     /translation="MKSTGKVGTIRYTLLTSRANRRITIRVGPDGILAIRAPRRVSRR
                     VIEELITEHREHLLNRIDEEVRDAHTYEDGDRFPYAGGMVTLQLRTGNDYRAYIEDDL
                     LVVSARNIQDKGRILHAIKTLYRAEARRRIGPLVEQWSRTLGVSAPSFTIRDSKKRWG
                     SCSKEGRLNFSLRSQALGEEELSYLVLHEVAHLVHFNHSGEFKALLQTHMSDYKIRQK
                     RMYAMQRASQLIY"
     gene            6548..6973
                     /locus_tag="PLF67_01000"
     CDS             6548..6973
                     /locus_tag="PLF67_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88620.1"
                     /translation="MSMMNLSLRQGLAYQKLPCEGSSAEDAYRALISFLDQAPAGSEG
                     VLLLSFEMNVLFLGTSAPPDEETLKKIAKAEKLDPAEGDHVLEPGHYRFIQIPLPASI
                     EELPLENLALDEGDLLYLRILKEGSFALVAQLWIRRRAE"
     gene            complement(6954..7898)
                     /locus_tag="PLF67_01005"
     CDS             complement(6954..7898)
                     /locus_tag="PLF67_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOE88621.1"
                     /translation="MDTLIYLAKLTVALILGSLFSKAIGKERLVRFSAIGQTVLVWVL
                     LFFMGINTGRIENIAGELGRIGLSALVLTLFGLAGSTVVSLAGSRLFLPKDLDTTIAV
                     ARRKEGSLLRYVWDLLKEPLFLISIVILGVLFGMKSTFFDWFKSSYVSYLLYVMLFFV
                     GMNLIQRSVSFKTVLRDPSVFLLPVYTIAGTLLGAFGASLATSFSFREAAGMLSGFGW
                     YSLSGILISDLGFPLLGSISFLSNLMRESFSFFLIPLFGRLGKRYYYPAVSCGGATTM
                     DVLLVLLSKHFGSATTIGSIYHGAATSLCAPVLIPLFF"
     gene            7980..9173
                     /locus_tag="PLF67_01010"
     CDS             7980..9173
                     /locus_tag="PLF67_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOE88622.1"
                     /translation="MTVLEISDTRRLITLADGRRIHLIGTAHVSKHSVDEVAEAIETE
                     QPDHICLELDEGRMKNKEEERSWSNMDIKKVLKEGKGFLLLANMALASFQKRMGDETE
                     TQPGAEILGAAHLAKEKEIPFSLCDREIQVTFKRAWRMSNLWNKAKLIATIISAVFSN
                     EKLSEEDLEDLKKADVMQQMMDEMAAELPAVKSVLIDERDRYLATKIYLAPGENKLAV
                     IGAGHMSGIVKTLEALDSNRLDTDVSEITVVPDSSRSSKALSWVFPVLIVSLLLYGFL
                     SFGRTEGIRMIGLWLAVNMSFTALGAILALAHPINIIVSVLASPITSLHPAIGVGMVS
                     GLLQATLRKPKVRDFENLNDDATKLKGWYRNRVLHILVVFLLTSLGGSIGNVVVFPFL
                     LARLA"
     gene            9217..9963
                     /locus_tag="PLF67_01015"
     CDS             9217..9963
                     /locus_tag="PLF67_01015"
                     /inference="COORDINATES: protein motif:HMM:NF027796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HOE88623.1"
                     /translation="MEVTMDYFSQANSAPLYILVGVVLLFVAISCVLFIRKSYLAGLK
                     MGMDRSVLRTTITSSALFTVLPSISILLGVIALSGSLGVPSAWLRLSVVGNLSYEAIA
                     AEAAAQAMGVSLKDAASLTTGHLVTILLVMTTGISWGIIATIFFGKSYSRKSTQLLNK
                     SKKKEGKSFADWAMVAMFIGFCGTFIGSYVAEAVVFKHFLPLLTALVAGLVMRGCEYL
                     VKEKQLAWMENFSLSLSMLIAMTAAVLLAL"
     gene            9974..10648
                     /locus_tag="PLF67_01020"
     CDS             9974..10648
                     /locus_tag="PLF67_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88624.1"
                     /translation="MEKSFDYEQYDDSIHRLGRITNAIGILSLLATPFLFALVLGASL
                     SWSGFAKGLLRVAITYLPITIVEFLIYVPILGPGGSYLSFITGNIINMKLPCAYNARE
                     IAKTQAGTPEDSIITTLAVGVSSLVTMLVIFIGVLLLIPLTPVLNNPTLRPAFDNLLP
                     ALFGALGAQYFFKSWKVSAIPLILMIALSIAIPALIAQTTIMMILIGAIAIAITYFFH
                     KKGALQ"
     gene            10645..11967
                     /locus_tag="PLF67_01025"
     CDS             10645..11967
                     /locus_tag="PLF67_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOE88625.1"
                     /translation="MTEREKRYARLLSKMIQVETISEPGIIHEEKFIKSEEVLANLFP
                     KVFSSFERLPIESGLLMRLKGKTEGEGFLLMSHQDVVSAEGEWTYPPFSGEIADGKIW
                     GRGTVDTKGALMCILQAAEELLEEKYIPEVDFYIASSWNEEVSGPGAPNAARYLHDHG
                     VRLQLLMDEGGMVVDEPVKGIKGRFAMIGTVEKGYGDIIAIAHGKGGHASAPPKNSPL
                     ARLAGFMNELEKHDPNTVELSLTLMEMFRRMGSRADGVLGFVLRNARFLKPLLKRILP
                     KKSPVLAAMMKTTMAFTMAEGSLGRNVLPQVAKVNINTRFIQHQGVEETMAILAPIAK
                     KYDIELKIEDHCRDLQEPVDFNGKGFKIVEETVNELFPGVVATPYVMTGGTDAYHYRI
                     VTDNAIRFAPLSISNEQHATVHAADENIDIAVLPPAVDFYKAIIKKVR"
     gene            11995..12870
                     /locus_tag="PLF67_01030"
     CDS             11995..12870
                     /locus_tag="PLF67_01030"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88626.1"
                     /translation="MNTSSTSYTPIDLPSLAKKVNPKLAKVIPRCMYRKLERLLHVKE
                     INEFFAAHHDDDARTFLDAVVSHLDLQIVIEGQGIEALHALEGERVIFASNHPYGGPE
                     AMALFSIVHRLFPDAKLVAQSFLKFIKPIETACVFNKKEVKTLMEAANNRSSLIFYPA
                     GYCSRILSNGEVFDYSWQSSFVRIARKQRMPILIIYTDGELSRKVHRWTRLRKLLRIK
                     LSIETIFLVDEMFKLKGKTLRMVVGSTITPEMIDEAKMSDRLLADKLRQYCYELRKDP
                     ELVFDPNIVPTLPQL"
     gene            complement(12993..14348)
                     /gene="radA"
                     /locus_tag="PLF67_01035"
     CDS             complement(12993..14348)
                     /gene="radA"
                     /locus_tag="PLF67_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOE88627.1"
                     /translation="MAKRSGDLQFVCSNCGRTEAKWLGRCPDCGSWNSFEEELIITPS
                     KGAKPLSTDEEPVRLEDVVVDPQFRYASGISELDRVLGGGVMKGSSVLIGGEPGIGKS
                     TLMLQLLQSTRNTSKVLYVSGEESPSQVKMRAQRLGLHLSKINIFCDTRLEVLEKVLP
                     SSKASLVVIDSLQTLSSDTLPSPAGSVNQIRHCSNALVSLAKRLGISLFFVAHVTKEG
                     TIAGPKVIEHLVDTVLYFDQLPSGIRLIRAAKNRFGSVDEIGLFSMDEKGLHPVANPA
                     SFFLSDRAEGPLPPGVAYTAVAEGTRSFLVEVQALTVPAKSGFSRIFSDRIDSARVTR
                     IAAVLERHAGLRLSDQDIYVNVGGGMKVGEVAVELALALALYSALTRTSLPKDLVSFG
                     ELSLSGEIRPVGFIQRREKTAKELGFTRSLVSKASKQSSLTQYGARTIRDALDAVREM
                     H"
     gene            complement(14361..15302)
                     /locus_tag="PLF67_01040"
     CDS             complement(14361..15302)
                     /locus_tag="PLF67_01040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE88628.1"
                     /translation="MDTKTLKDGLYAVMHTSKGDILLALEYKKTPMTVANFVGLAEGS
                     LNKDKPFYDGLKFHRVIANFMIQGGCPLGTGTGGPGYKFGDEFDPSLKHDRAGILSMA
                     NSGPGTNGSQFFITHVETPWLDNRHSVFGHVVEGQKVVDSIAQGDTIKKVEILRVGSD
                     AEAFKVTKQIFTSYIVESENKRKKVLEEARANVEAELKNRYPEAIRTKTGLRYVVKKK
                     GDGERFPKMGTRVKVHYQGSLLDGRIFDSSIQRGEPTEFRVGEVIEGWNEALVTMSAG
                     EERTLIIPPELGYGEMGYPGIIPPNSYLIFEVHLLSF"
     gene            complement(15314..16243)
                     /locus_tag="PLF67_01045"
     CDS             complement(15314..16243)
                     /locus_tag="PLF67_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOE88629.1"
                     /translation="MESKRKIGLGKGIGSLMQDFSYDSVLDNALSSAKKEEKGDRVLE
                     VELDAVRPNPNQPRKEFDNATLEELAQSIKREGVLQPILVEEIGPNRYSIVAGERRYR
                     ASKMAGLKTIPVLVKDVTQMQRLEISLIENIQRESLNPIEEAQAYAYLIQEAGITQEE
                     LARRLGKNRSTISNSIRLLQLNPVMQDELLKGKFTAGHARAILSVVNPADREILYKTL
                     LERDLSVRATEEMATQFNQGRRVAHQKKKRSKKEAEKSLEILAAEESFLMAFGTQVEI
                     KGTIKKGRIEIPFSSTEELERIYQILVPNEHLF"
     gene            complement(16233..16994)
                     /locus_tag="PLF67_01050"
     CDS             complement(16233..16994)
                     /locus_tag="PLF67_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOE88630.1"
                     /translation="MKTKIILFLNQKGGVGKTTSAINLGASLAEMGKRTLLIDIDSQG
                     NLTSGVGLDARKPGVYEVLAGSAELSEVIQQTAVRNLFAIGSNIHMAGLAIELVNQDR
                     REYFIRDAFSPLEGNWEYIIIDCPPSLGLVTINAMVWANEIIIPMQSEYFAMEGLTLL
                     MHTIGNIKKSLNPELEVLGILFTMYSKRTRLSRDVVDDIVSYFDDLVFDTMIPRNIRL
                     AEAPSHGLPINQYDGSCNGAKAYRALAKEVIKRGK"
     gene            17089..17313
                     /locus_tag="PLF67_01055"
     CDS             17089..17313
                     /locus_tag="PLF67_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOE88631.1"
                     /translation="MLEDKVKQAIQDIRPALQNDGGDIEFVSLAGKEVTVRLVGACAG
                     CPMSQMTLKGGVERYLRNFVDPQLTVVNER"
     gene            complement(18036..20540)
                     /gene="gyrA"
                     /locus_tag="PLF67_01060"
     CDS             complement(18036..20540)
                     /gene="gyrA"
                     /locus_tag="PLF67_01060"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HOE88632.1"
                     /translation="MENRTIKVDVASEMRTSYLNYAMSVIVSRALPDVRDGLKPVHRR
                     ILYDMYEMGLRANSSYKKSARIVGDVLGKYHPHGDASVYDALVRLAQDFSLRYPVVNP
                     QGNFGSIDGDPAAAMRYTEAKMSRIGEEMLSDIQKETVDFKPNYDDSLTEPSVLPGAF
                     PFLLANGSSGIAVGMATNMAPHNLQEICAAANALIEDPEITLMELMEHIKGPDFPSGG
                     VICGMRGIVDAFSTGRGRIITRGKYEIEESARGGDVIVFTEIPYQVNKADLVKKIDDL
                     RKDGSIPMISTIRDESDRNGIRIVIELKMGAEVMVVLNQLFQRTALQSNFNVNNLALV
                     DGRPQLLGLKDMLHYYVKHRKDVVTRRTTYDLRKAQERAHILRGLKIGLEHIDEVIQI
                     IKDSPDNSIASQRLIAAFALDEIQAQAIIDMRLGRLSHLESSKILEELEDLEAKIAYY
                     NELLSDEGKLMGVVQAEINALPQNLAPRDKRRTEITVEELGQATMADFIKEEDVVVLI
                     SNKGFAKRVPTEEYEARGRAGMGVRSAKLQDGDFIDHMFVASTHDYVMFVTNMGKAYY
                     TKVFDIPEAGKYAKGVSIKNFIQIETEEKVTSIICFKEFTDEQYLMMATLYGVTKKVS
                     LHNFVNARSRGIWAIVLDEGDELLSCELVEDGDEVMFVTKGARALRFQSDQVRAMGRA
                     TRGVRGIRLVDDDQLAGMVKVEEGKHILVITANGQGKRVEFDAFIPHNRGTQGQRILR
                     LRKNDEIIGVLGVSETEDVVCVTLLGQTLRIHVDTISIQGRNAGGVRVVRMKWDDDSI
                     VAIASTDRDDEAEVETPDEEGDQADSDIDVEDDDEA"
     gene            complement(20564..22594)
                     /gene="gyrB"
                     /locus_tag="PLF67_01065"
     CDS             complement(20564..22594)
                     /gene="gyrB"
                     /locus_tag="PLF67_01065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOE88633.1"
                     /translation="MNDGSFSNGNADLSAGAKATGAVKPPDQYSASSIQVLKGLEAVR
                     KRPGMYIGSTGIDGLHHLVYEVVDNSIDEAMAGYCDTITVTLSRNEDGRQFCTVEDDG
                     RGIPVAIHPIEKKSSLEIAMTQLHAGGKFDKNSYKVSGGLHGVGVSCVNALSIWMEVI
                     VKRDGGIFRQLYSQGNPTSEVQRIGDTDETGTKVTFSPDYTILEENAFSYETLVTRFR
                     ELSFLNKGITITVSDTRFEEPKKQTFHSEGGIVEFVSYLNEYKRTLGETPISIEGERD
                     NIKVEIALQYNDKYDEKVLTFVNNINTKEGGTHLTGFRTGLSRVINAQLQKNQKLLKR
                     YEDRLEPSDISEGLTAIVSIKIPEPQFEGQTKMKLGNSVVTGVVFSLVYEKLGNYFDE
                     FPAHAELILGKIINAALGRIAARKAREQIRKKSEGGGLPGKLADCSEKDPALCEVFIV
                     EGDSAGGSAKQGRDRRFQAILPLWGKMLNVEKAQEYRVLGNDKLQPVISTIGAGLTRY
                     NNMGKDEDLDSDVTFDITKARYHKVIIMADADVDGSHIRTLLLTFFFRYMRPLIEAGY
                     VYFAMPPLYKITIGKNVSYAYDEEARIRILEEAGPDAAKASIQRYKGLGEMNPEQLWE
                     TTMNPETRLISQVTLGDAEEADRVFSMLMGEEVEPRRAFIDANAVYVSNLDV"
     gene            22794..24191
                     /gene="dnaA"
                     /locus_tag="PLF67_01070"
     CDS             22794..24191
                     /gene="dnaA"
                     /locus_tag="PLF67_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOE88634.1"
                     /translation="MSDQQTSYYEFWLETAKRIQETLPPQEFSTWFSRISYLSSKKGE
                     VTLAGPSQFVLDTVTTRYRSIIANTLSELTGEEIAVEFVVSDKGKKAVRPMDGDETAE
                     EARKEKKADDLAKKISKRKSEANLNPSYEFESFVLGDNSAFAYNASLAIAKNPGSSYN
                     PCLIYGDVGLGKTHLLSSIGNYILDHTPECKVMYVTAETFTNEFIESIGSQKTQQFKN
                     KFRNVDVLLIDDIHFLQGKDSTQEELFHTFNNLYEAKKQMVFTCDRPISELKNITDRL
                     SSRFERGLNVDLQPPNYETRVAILKKKLQEHGSSLSEDIINYIAFNVVTNVRDLESSL
                     TKLVAYMDLIGEELTFEKAKQLLGILPAMANITRDTLSIGEIIKTVGDYFNVSTFEMK
                     GKKKNKSLVQPRQIAMYLARQITDFSTTEIGMEFGGRDHTTVMYANNRVESLMKGDEA
                     YAATVNKLRRQLTSS"
     gene            24415..25521
                     /gene="dnaN"
                     /locus_tag="PLF67_01075"
     CDS             24415..25521
                     /gene="dnaN"
                     /locus_tag="PLF67_01075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOE88635.1"
                     /translation="MKFISTKEAILSEIIYTVDFTTQKNSLAITSNVYLQTLGDQLLI
                     KATDGKVGFSTSITVDVQEEGKALVLQEKLLEILRVLPDGDINFSMKDGFFTIVPVGH
                     GIDFKIRMIEADQFPALETIDNDRFFSIAQSSFLEMSEQVIFAVSDDTSRYFLCGVYL
                     ERSEGGITMVATDGRRLSIVERVFEEKLPSFAPIIVLPKFFQILRKIGTGEGAVELAI
                     TESILFARIGGRTFYITLIREQYPNFRRVIPEQQSYRCTMKIQDMEDALRRVAILIES
                     KAKRLFLDISEGGVLLTSDVSESGEAKEIVPCQYEGPDCKISLNYTYLLTPLRVMEGE
                     YFSINFTEPNRSMTVKPESGRDYMHIIMPMQLNP"
     gene            25521..26609
                     /gene="recF"
                     /locus_tag="PLF67_01080"
     CDS             25521..26609
                     /gene="recF"
                     /locus_tag="PLF67_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOE88636.1"
                     /translation="MRFCTITTNQFRNLKEEPLEVDASKVLLIGPNGQGKTNLLEALY
                     TLCYGSSFRTTQIKELATYGFKDFRIEGTYRDERNLLHTLTLTFKDNKRVITLDGTVI
                     RDRKELIYNIPTIVFSHDDIFFVKGEPEYRRKFFDQTMSMYNPLFFDDHRRYRQILRQ
                     RNQAIKDEQYSLLPIYDQQLARHGIAIQRERIRSVYEFLQIFPEIYQEVSRSDTLLDI
                     EYRPSWEECATEEEVVHCLESHRRRDIKLQTTTSGIHRDQFMITEDGYPFANKGSTGQ
                     LRLASLALRVAQMAYFTKKTDKEAVILVDDVLLELDIPKRERFLTILSGYSQAFFTFL
                     PEERYIRSFETSDRKVYYVSEGAFTLGQ"
     gene            26581..26922
                     /locus_tag="PLF67_01085"
     CDS             26581..26922
                     /locus_tag="PLF67_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOE88637.1"
                     /translation="MKEHLLLASDEKPRLSKGDDPLLGKEVLNTVLKTLDIDLEEPRY
                     GLTASWEEIAGPELFPHVKVVDIRHGSLVLRADHPSWANLVMLQKKRIVQAVRKRYPS
                     LGITTLSVLGA"
     gene            27019..27186
                     /gene="rpmH"
                     /locus_tag="PLF67_01090"
     CDS             27019..27186
                     /gene="rpmH"
                     /locus_tag="PLF67_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOE88638.1"
                     /translation="MSYKGKRTYQPSRVKRNRKFGFRARMATKGGRLVLARRRAKGRY
                     KLSVADEKKPF"
     gene            27167..27517
                     /gene="rnpA"
                     /locus_tag="PLF67_01095"
     CDS             27167..27517
                     /gene="rnpA"
                     /locus_tag="PLF67_01095"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOE88639.1"
                     /translation="MRKSLSKSEIVKRRSEIDRIFKTGKKISSRGMNLIVTTNDLPFS
                     RIVVIPVRGYGSSVKRNRIRRQIKEIWRTEKDRFLPGYDFAFVVYPGRNVDHALQRRH
                     VLTLCKQAGVMNPP"
     gene            27574..29355
                     /gene="yidC"
                     /locus_tag="PLF67_01100"
     CDS             27574..29355
                     /gene="yidC"
                     /locus_tag="PLF67_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOE88640.1"
                     /translation="MDRNTILAVILSVLVITVGMTIQATFFAPSAQEEVAEEAANVEE
                     IYLQPEAYSKLPSNLAWGTDLPGSLIALESPANSSPFVYETDIFLITFDPKGASISSF
                     KLKKHLDKGEPVELLFGGSNANNAFLMYAGSDRTQPIDAPFNYRIAGNQVIFTQTFAI
                     KGEDVPFTLTKTFTFGESEYLFQIDVNLVNSENKAIPLAFDNTAYTIGFEPQIGPSFT
                     SIDNRYSYRRFYIREDGAKKKATITVKNGIYTTEKPLSWVALTGKYFSMIAIPDATNY
                     TVTLSEDTKTDIPLESNVFFSRPAIRSADQTDTYRFYIGPQLKADMVPYNDRASNSFG
                     LSNLFLEDTLDSSSWLGWLETILKTILNLLYKVIPNYGVGIIILTFLLKLILHPITKK
                     GMESTAKMSALGPQMEEIKARYPDNPTKQNEEMAELYKREKINPMGGCLPMLLQFPIL
                     IAFYGLLNKHFELRGAMFIPGWIPDLSVPETVATLPFNLPFLGNEIHLLPILYTVSMI
                     YSMKITQSSSGGAASQQGMMKMMTYGMPIMFFFVLYNAPSGLILYWSVMNVISVVQQL
                     YTNKKKQKEGEQEKVIQFQPKAKGKKR"
     gene            29383..30054
                     /gene="jag"
                     /locus_tag="PLF67_01105"
     CDS             29383..30054
                     /gene="jag"
                     /locus_tag="PLF67_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOE88641.1"
                     /translation="MIREFEGRTEQEAIAKAIEELNIEREDFDVEIIEPARKGLFKKN
                     SVKIRIHFDDGMDEEESYYDEGEDLDEPFDSDLEDKVLTFVATVLEKMGYAGNVSIAF
                     RKERKIGLRIDSENSNIIIGRKGKNLDALQLLTNVFVGQQNSNVRVIVDSENYRIRHE
                     EQLIRLAYRTAEQVARSGQSRLLEPMNPYERRLVHTALNDFGGVETKSEGDGLYKQVR
                     IIATN"
     regulatory      30078..30280
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            30349..32418
                     /locus_tag="PLF67_01110"
     CDS             30349..32418
                     /locus_tag="PLF67_01110"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOE88642.1"
                     /translation="MVTIIKRDGREVFCEARKITDAISKAAHAAGEPIDSVALTNRVL
                     ERISHRTAVEVEEVQDLVEEALIEANYPKTAKAYILYRKERQRIREGNALIKATRNLF
                     DSYLEDKTWQIKENANARKSVNGMNNYIRERLTESYWLNDIYPKTIREAHLEGTLHLH
                     DLGFFGPYCCGWDLRLLLEKGFGGVEGKVSSKPAKHLRSFLGQIVNATFTLQGECAGA
                     QAWSSFDTYCAPFVRSDNLEYKEVKQAIQEFVFNLNVPTRVGFQCPFSNLTFDITPPK
                     TLSEQPVIIGGALQDTCYGEYQEEMDLINKAFCEVMEEGDASSRVFTFPIPTYNVTKE
                     FPWESEVVDAIFSMTAKYGIPYFSNYVSSDLSPEDALSMCCRLRLDTSELRKRGGGLF
                     GSNPLTGSIGVVTINLSRLAWESADENEFREKLLSAALLAKESLEIKRKIIEGETDRG
                     MYPFSKVYLSSVKESQGTYWANHFSTIGVLGMEEACINLLGSSLLEDAGQAFGLRTLA
                     YLREVIQSFQETSGGVLYNLEATPAEGASYRLANLDKKAYPEIVTAGEKEHYYTNSSQ
                     LPVSSTKDIFEVLEKQDELQCQYTGGTVLHLYLDQRIKEAALAKLLVKRVFSRYRLPY
                     LSLTPTFSTCTDHGYLVGERKTCPHCGKATEVWTRVVGYLRPVEDFHPGKQEEHSQRS
                     FFKAEAV"
     gene            32415..>32887
                     /locus_tag="PLF67_01115"
     CDS             32415..>32887
                     /locus_tag="PLF67_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOE88643.1"
                     /translation="MIRGIEGVSFLDYPGLLSCVLFLGGCNYDCFYCHNRSLLQPGEE
                     VLSLGEVESFLAERKGLLDALVVSGGEATLHKEITSLFSIGKGLGYLTKLDTNGSRPE
                     ILKTLLEADLLDYVALDIKAPPERYREISGGSFLPVSKSLNLLKRHREHEKTFSF"
     assembly_gap    32888..32987
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32988..33253
                     /locus_tag="PLF67_01120"
     CDS             <32988..33253
                     /locus_tag="PLF67_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88644.1"
                     /translation="SLNLLKRHREHEKTFSFEIRTTMAPTLGREDIEQLASIVGEVPR
                     WVLNEYRVPRTFKEEDRDRVHQKYLCKDELQGLLPLHPALCLG"
     gene            complement(33245..34366)
                     /gene="metK"
                     /locus_tag="PLF67_01125"
     CDS             complement(33245..34366)
                     /gene="metK"
                     /locus_tag="PLF67_01125"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOE88645.1"
                     /translation="MFLRNRVLTSESVGEGHPDKVCDLIADAILDACLEGDPNSRVAC
                     EVFATTNTVIVGGEITTNADIDVDAIVRDTVRRIGYTIEGIGFDAESLSVTNLIGRQS
                     ADIALGVDHDGAGDQGMVFGYATKETPVLMPASVYWVHELLKRAASVRRKTDFLRPDA
                     KGQISLAYRHGRPHHIDAVVLSHQHGQEIAHKDLVDFLKKEVIIPTLEPSGFFDRNTA
                     LYINPTGRFVIGGPAGDTGLTGRKIVVDTYGGIGHHGGGAFSGKDPSKVDRSGAYLAR
                     WIATNLVRSGLCTVCEVQLSYAIGVAEPISIAVETFCTGVMSEDELIRIIRRNFDLTP
                     KGMIEALNLRAPIYRRTTNYGHFGKPDLPWERERILSLP"
     gene            complement(34521..35519)
                     /locus_tag="PLF67_01130"
     CDS             complement(34521..35519)
                     /locus_tag="PLF67_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOE88646.1"
                     /translation="MVYGIGNPLIDIIINASDEDIARLGIHKGTMALIDEERREELLQ
                     MVEDRNVSYSCGGSCPNTIIALASLGVETTLAGKLGNDPYATIYRERLASLGVRDELV
                     CTDKQKTGSTIILISEDSERSMNTFLGANRLYEAADVSEESVKNAEIFHFTGYMWDTA
                     SQRRAIERALMIAKEHHTIVSFDVADPFAVGRYHKAFLHLIKESCDIVFANREEARIL
                     FDNYDPYECCRSMGKLCRIAIVKDGNKGSFIAHEGTIIKIPPKGTPNPVDTTGAGDVY
                     AAGFLYARTKGMDIEASGMIASILAGEIVTQRGAQFSEEKAKELRILLESGGWRYR"
     gene            35611..36900
                     /gene="murA"
                     /locus_tag="PLF67_01135"
     CDS             35611..36900
                     /gene="murA"
                     /locus_tag="PLF67_01135"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOE88647.1"
                     /translation="MSEYRIIGGNAASGEVLISGNKNAALPCLAATLLTDDPIRLSNV
                     PEIEDVKVMIALLESLGSSVVREGKGVYTIQSGSGGASLPKDLVQAVRGSILLLGPLL
                     ARNGEVTIPPPGGDVIGLRRLDTHFIGLSALGASAKLNEDGSLHFKITGRGLIGNDIF
                     LDEASVTATENVIMAASLAEGETTVFNAACEPHVQDLCHLLVRMGSSIEGIGSNRLII
                     TGSKKLTGASYTLGFDYMEAGSFIGLAAATGGEVLIKGIDHRHLRILHLGFERIGIAW
                     EHDGPSSILVPKKQRKVLVKEVGGQTTKIDSAPWPGFPADLLSILTVAATQSRGSILI
                     HEKMFESRMYFIDWLIRMGADIILCDPHRAVVQGPSVLIGSVLSSPDVRAGMALVIAA
                     AAARGESVIQNIYQIERGYENLSEKLTSLGIDMVRSG"
     gene            complement(37275..38396)
                     /locus_tag="PLF67_01140"
     CDS             complement(37275..38396)
                     /locus_tag="PLF67_01140"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE88648.1"
                     /translation="MSLFVLSLDWRTKERFKLQFTQPYLQLFNLPAALFEALESETPD
                     MIIIDTKEPKITAGQILRHLGESRCSAPIIIFTRDEPVQNFRNPYDLKIRLMHKRTVN
                     WEEVFQEICRIGKTELVKESSPVWSSGLVGESKLMVELRSLLEFYATKDCPVHIYGET
                     GTGKELAAEYLHRRRFPQRHIIAINCSLLNDAVGKSVFFGHAKGAFTDATNELIGLLC
                     QANNSTLFLDEVENLPLIFQAHMLRLLENGEYRRLGDTKLRSSDFRLVTASNEKLLRL
                     VAEGQMRKDFFYRITDAQIHMPPLREHKEDIPLLIRHFYSTLSERRPVTDTSIKLLQS
                     YHWPGNVRQLFSVLRRASLHAKNNRWIEIDEQEFAEEEQ"
     gene            complement(38514..39284)
                     /locus_tag="PLF67_01145"
     CDS             complement(38514..39284)
                     /locus_tag="PLF67_01145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOE88649.1"
                     /translation="MRVYRLPSTYDGEEEIALKGKEAHYLTNVLRLKAGSRLLAQDCN
                     GVRYQGVITASASASCTIRLTVAGDTSASTDALPSYEEPFTPLVLLLCLLKGRKEEQV
                     VRQATELGVGEIVLVSSEYCTVRLDKKNTRARFSRLEAQITEALQQSGSRVPTTLLDR
                     VLDLRELAEWWAGRGPLLFLHQSSRSQSKDLSTYAKTFDPNAPIGVLIGPEGGFSEKE
                     CVFLEKAGFLPVFLKSNILRSETAAIYALGSLQELLHR"
     gene            complement(39281..40705)
                     /locus_tag="PLF67_01150"
     CDS             complement(39281..40705)
                     /locus_tag="PLF67_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOE88650.1"
                     /translation="MKKFRAFHIFVFILIALTLAPVFAGGDPEPLITGLTSGRFAQDK
                     ISTDISALERLYRYIDAIYIEEVDKDKVFTDLATALVASLDDPYSFYVKPSDAKEYEE
                     GTSGIYGGIGTYLSKPNPDSIDPDDPETYMITIVSPFPGSPAQRVGLRAGDLISHIDG
                     EPVDALTSLESSRKLRGEPNTSVTITVYRMGTSFDLTLRRELITAPTVDSMLLEQDLL
                     YIQISEFSPQTGKQLLEHVRKYDLNEIKGIIIDERNNSGGAVDGAMQAANIFLDAGKT
                     IVTIQGKRGSSRDQRYLSSNKAEVSEEIPIVVLTNGGSASSAEIFAAAMRDNNRATLV
                     GEKTFGKGIVQDVFRFGEGFAQITTAHYYTPNGENIHKIGIEPDVFVESLVMSEEELE
                     PFAELMEEKVVSSFIAAHPEPSDANIAAFVNANRERGIRDEVLAILLRNEYQAKIPYD
                     ERKLADPAFDPQLQAAIDVIRARR"
     gene            40788..41735
                     /gene="lgt"
                     /locus_tag="PLF67_01155"
     CDS             40788..41735
                     /gene="lgt"
                     /locus_tag="PLF67_01155"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOE88651.1"
                     /translation="MAMYISFPSWIKPEIFSFLPIRWYGLMYILAFATAYLFIVIQAK
                     NGEIALTREDALDLVMWCVVGLILGARLFSVLFYDGTTFYLTHPHLIFWPFRNGKFVG
                     LPGMSYHGGLFGAAVGGWLYSKKKRIPFLEIADTVVYSVPLGYTFGRLGNFINGELFG
                     RVSTKPWAMVFPDAPSFSTNYEWVRRIADELKIPYELGQYINLPRHPSQLYEALFEGV
                     LLFFFLWFVIRPKRREKPAGTGLAWYLIGYGTVRFVIEYFRSPDEHLGYIIAWGRGSD
                     TIALFQSVFNISMGQIFCLIMIVLGVAIRLITHYRSTKQ"
     gene            41732..43510
                     /locus_tag="PLF67_01160"
     CDS             41732..43510
                     /locus_tag="PLF67_01160"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOE88652.1"
                     /translation="MNIINARVESLRRAMADAGLDGWIINGTDPHQSEYVPERYRSRA
                     FISGFTGSAGLALITKEKALLWVDSRYFLQAADQIAGMVFEMMKIDTPSHPDPYTYLK
                     MNLPKGSVIGIDEATIPVSERRRIEGVFQNTLVLKPMKDILDAVWTDRPALPEHEVVE
                     VPVNYAGSSMQEKLRQVRSLMIEAGATHLLLSTLDDIAWLTNLRGSDIPYNPLFMSYM
                     LISTERACLYTGEKRFTEQLRARLEEDLAIRPYTQLQDDLPNLWKHAVLYYNPQKTSV
                     MVERSFGPYEKIEGREFTTDLKAQKNEVELEGMRRAHLLDGVALVNFLGRLDRENGSY
                     TELELAKRLLDERSRNEHFLGESFNAISGFGAHGAIVHYAADEQSNAKVEGDGLLVLD
                     TGGTYAFGTTDVTRTILFGKPTEEQIRDYTLVLKGHLALASARFPEGTNGYQLDVLAR
                     QHLWREGLSYLHGTGHGVGFRLNVHEGPQSISPKPIVAPLKEGMVVSDEPGIYRSGSH
                     GIRIENLLAVQEDEKTEFGTFYSFEVLTLCPFERDLIDPEMLTEEEIEMVDAYHAWVY
                     DELYELIDDEAIEYLEYATQSFLGEE"
     gene            43512..45158
                     /gene="gpmI"
                     /locus_tag="PLF67_01165"
     CDS             43512..45158
                     /gene="gpmI"
                     /locus_tag="PLF67_01165"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOE88653.1"
                     /translation="MVKPLQKLGKIKRKGPVVLLIMDGVGFGKYKEGDAVAAAVTQTL
                     DRLYRENPWTKLKAHGTAVGLPSDDDMGNSEVGHNAMGCGRVFSQGAKLVANSIETKA
                     MYEGDTWKKLIRNVKEHDSTLHFIGLLSDGNVHSHIDNLKAMVAQAKVEGVSRVRVHA
                     LLDGRDVGELSALEYFQPFEAFLEELNDEDFDARIASGGGRMVITMDRYNANWKMVEK
                     GWHTHVLGEGRLFASPTEAIETLRKETGAIDQDLPPFVIAEGGKPVGTIEDHDSVILF
                     NFRGDRALEITKAFEAEELIEFDRKRRPNVEYAGMMEYDGDLHVPAQYLVSPPAIDRT
                     LAEYLTKSGVRQFSISETQKFGHVTYFFNGNRSGKFSETLEEYVEIPSDTVPFEDRPW
                     MKCAEIADRVIEEIESGKWDLIKLNFPNGDMVGHTGVYQAVVCSMEGMDLQIGRIEKA
                     VRDAGGVLMITADHGNADDMYEHTKTGEVARKEDGTPKAKTSHSLNPVPFILVDPLYK
                     GEYEPTLKEGLGISSVAATVMNLLDFDPPEFYDSSIITMK"
     gene            45217..47274
                     /locus_tag="PLF67_01170"
     CDS             45217..47274
                     /locus_tag="PLF67_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE88654.1"
                     /translation="MGIDDRLKRLSRLQGGDPGIFALAVHSFIEGELRRRYDLPYEEE
                     PSFNALSDRLVEEVGEKSRQFLANLKAAHKAVNEVRHEFNALGVDHALMAAAHLYTFC
                     YFIDIDEGALQTLYKIKRELDDTWTRSESRGELITSNQILQTELQKILAEKGSLSDQL
                     SELKEREEEREQVDDRIHSLEHQIRNLLKDRDRNEKKIKELRDERHALQVQRRSLEKQ
                     TRNLEEYLHVMRTMTSYTRTRRDYEHLILRLSAQQEEALRRIDLSSDFLITGGPGTGK
                     TLVLLKAIGKKSEKSNVALLTYTNSLAKYDQYLTRLLGIEMGQIMTIHHFLRERIEEG
                     ESIKVLAGEDTDILIRLCEPYASLQWTAKDIYDEIVHVLWAFGHTRQRYCIDLVQRSG
                     MEKELDEAMRSRIWEAKEAVEAFIEETKQLPFQYFPIQALKVAEEHPVTIFDHLFIDE
                     AQDATAMTLTALKRFVSKSIILASDGEQAIYQPGFSFTQAGIDVSDNRVILQENYRST
                     LQIHRLAQRFERRSESEQSRALRDGPNPELHRGRDGSVLQKLVVERAMLFIERLEYEE
                     ENICIMCPSSSCYPELEQALTKAGISSVRVNDERFTFGEECHGHLRLSTLHSAKGLDI
                     PVVLLYLPFLPRVNESYDESVNNELIRKLLYVTMTRAIDHLNVFTLVSPKEEIIRELI
                     AAF"
     gene            complement(47271..48032)
                     /locus_tag="PLF67_01175"
     CDS             complement(47271..48032)
                     /locus_tag="PLF67_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88655.1"
                     /translation="MSKGVRFTFNLIQGLVTMALSFVVIVTLIASQDGHLVLEARAHL
                     WPALIHIWITFLVSLLLATRYRANLGSEAELLPVLFLTIVLANIKILPLHQAITGTFL
                     LDGGVLTFIYHGSLLYTSFLFLIGSLLFLRLSPSRIGFITFVGAAGSALLALLVPSSP
                     NDPSFLLEAGVSDSLFLSICITLTVVAALSYLVIALGEHTKRDTRFKTVAFLLLTVGN
                     AFVSISQRTFYNIIGLIIYGAGATILVLVSRSYHI"
     gene            complement(48029..49276)
                     /locus_tag="PLF67_01180"
     CDS             complement(48029..49276)
                     /locus_tag="PLF67_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOE88656.1"
                     /translation="MKTWISYLAATAFGLAATLVFGESSFFQQATYTVTAVLVQLGGF
                     ILIPLVFFSFSAGIASLRKDGKGCTFARTTVFWSVFSTLLLVLSAAAVFRFLPLPFPA
                     SSSAGTDPGTFSSVGLQSFSTFLGHLTIVNPFYTLVKADDFLLPVIIIAFFLGWFLKP
                     NVEIIRPAYVVMNSFSETFFRLARAWAQCGAIFLFFISSHWLISVAKEGTVFVMLPFL
                     MILGLGVLLALFVILPLLLAIFSLFSVNPYRILYRMLAPALAGMFTGSIFFTTPTTMS
                     LARQNLSVQKRVSGTAIPLYTLIGRGGSALVSTLATLTMIYSATGSVPEDMILLTIAL
                     ASALFSFVSPLYVGFEVFFITTLTLNFLKIDLYGAHMSMVALMPILNGVGILIDTYVA
                     SFGAAYTCSRMGVLVKTPHRDIV"
     gene            complement(49399..50493)
                     /locus_tag="PLF67_01185"
     CDS             complement(49399..50493)
                     /locus_tag="PLF67_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOE88657.1"
                     /translation="MEPYHIDRTALEHFCTHVFIALGLSEEEAVDSSEILVSADARGI
                     ASHGVARLWRYENGLRLGIMSGGIQPTILRETPISLVLDANGAMGMSLSRKTMDQVIE
                     KAKVSGVGFASIRNSNHFGIAGYYSEMAARSDMIGIAMTNTAALGVPTFGTTAMFGTN
                     PIAVAIPGHEGKLFSLDMSTTGVTRGKIEVYNREGHPLPSGWAVDPTGAVTSDAARLL
                     DDMLYQRGGGLLPLGGAGEELGGHKGYGLAVLVDMMTAILSGGTFGKSVKDSEATSAR
                     VCHFFGAIRLDLFRDPDELKNDVDALLSELNETPVAEGHKRVLWAGQKEQEAEARSDV
                     EGVPLSANVVSQLKSIGKELGIVFPLPLSK"
     gene            complement(50499..53840)
                     /locus_tag="PLF67_01190"
     CDS             complement(50499..53840)
                     /locus_tag="PLF67_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE88658.1"
                     /translation="MIPRDFRISVRTRTELDFFPPLFPSDAHLNELYQEATELAYRYN
                     EKVRSKGGNDYLSSGKLHAVAVLHQLYQSVLSNYLNTHEPDFFSRLGTLVGKNHDIEE
                     VLTFYMKEFPSPLLVSKSYPVEFYYLESLRGYFIHQVMEENPALIAASKPLLKPEGLF
                     FPKAHQALTALMGGYAQQRTAAKDDDLFSFLTRPARLYPDSLLDQIRYILEHWADMID
                     SELKRLLLRAIDYVQEEEKPHFPPGGPAPPMPVIDYSLFDHEYEAFTADRNWMPNVVM
                     IAKSTLVWLDQLTKEYGYPIDTLDKIPEREIELLAQRGFTALWLIGIWERSSASKMIK
                     NLTGNPDAEASAYSLRNYEIASLIGGWGALDSLRHTCSKYGVRLASDMVPNHTGIDSD
                     WVMSNPDLFIWQRHPPFPSYTFNGPNLSSNPNVEIKLEDHYYDRSDAAVTFQRRDLNT
                     GETHYIFHGNDGTSMPWNDTAQLDFLNPKTREMVYQQIKHVAMNFPIIRFDAAMTLAK
                     KHIQRLWYPRPGRGGDIAGRVPHSLDDETFHRLIPNEFWREVVDRINEELPDTLLLAE
                     AFWMMEGYFVRTLGMHRVYNSAFMNMLKNQENKKYRDTIKNTLTYEPEILKRFVNFMN
                     NPDEETAIAQFGDGDKYFGVCTLLATMPGLPMFGHGQVEGFREKYGMEYRRAYWDETA
                     NQHLVDEHYRRIFPLLKRRYLYSDVEYFELFDLVHDGHVHESAFCYVNGTDTERSLIL
                     YNNQYEKVEGTIKHSSPKLIKQNGGKHTATKSLAESLGLTVSGRRFVIWDSFTDKLTY
                     MSPSLKLFDDGLWVHLWGFETKVFLNIREVEDSDGVYAELYERIGGQGIANFEEEIMA
                     LRLRPVTEAMENLRSQSFFSLLSSIFDRTSSSKAERTLLLSLGEAYAHLTTAYELLHP
                     QTKKILNHPPRDPDVKAIMETVKRLNTLFADHSTRLFSQSRLLLDELEVIVSSAFFLN
                     PFVGDDAGIEEVITLAERLQLCRFYAEKLNKAGFVGDDQRKACQSGAIVVCAHRAYRK
                     GEKPQETLARLLEEERVRTYGLVNEYQGVIWFDKERMQELIFLSALSIAMNEPEFDPT
                     SYVKTLFDAQLNASYRLKRLLALPE"
     gene            complement(54193..54714)
                     /gene="pth"
                     /locus_tag="PLF67_01195"
     CDS             complement(54193..54714)
                     /gene="pth"
                     /locus_tag="PLF67_01195"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOE88659.1"
                     /translation="MMKLIVFLGNPGKIYEPTRHNVGWMVAKEAYGSIAWSEKFSAKI
                     AKDGPIHLLKPLTCMNESGRSVGLASSYFGIKADEILVVSDDLELAFGTIRLQQGGGL
                     KGHNGLKSIQSALGSGDFWRLRIGIGRPIHGTVSSFVLSRFTPEEEISLPLIVREAAK
                     LLDCRTPTQRILL"
     gene            54778..55110
                     /locus_tag="PLF67_01200"
     CDS             54778..55110
                     /locus_tag="PLF67_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HOE88660.1"
                     /translation="MYALVFFVPTTQLEEVKNALFAKGAGRLGSYEQCSFQVRGEGQF
                     RPLAGSSPFLGGEGVLEKAEEWRVEMIVSKEVVNEVVEALLSAHPYEMPAYHLYPVLT
                     KESFDGHS"
     gene            55097..58531
                     /gene="metH"
                     /locus_tag="PLF67_01205"
     CDS             55097..58531
                     /gene="metH"
                     /locus_tag="PLF67_01205"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOE88661.1"
                     /translation="MGIREEIRHRVLLLDGAMGTMIQSLRLPPSAFTYRGRTAAGLAE
                     VLNLTQGEAITSIHRAYIEAGADIIETNTFNANRISLSEYNLEDEVAPINRWAVALAR
                     EASRDAERRVYIAGVLGPTASGLSFSAKVDDPTARTHDFDFFIEAYREQAKALIEAGC
                     DLLLIETVFDTLVAKSAIIACREELEAAGKDLPIMVSVTFSDGSGRTLSGQSLGAFIA
                     TLAPFDLFSIGLNCSTGPKEMIPLLNELHAISPCYTSAHPNAGFPDEDGRYTLAPDHL
                     AGELKPLLAEGFLNIVGGCCGTTPDHIRALKRILPASRPRSVPPKKPALLLAGLDPVE
                     VKRDTLLIVGERTNVAGSRIFRRRIERKQWEEALLVARDQIAEGADLLDICMDDPLLD
                     SKEAIVSFLRYIASDPTIAKAALMIDSSDWSVIESALGEVQGRGIVNSISLKEGEELF
                     IRRAKKIASFGHAFVVMLFDEAGQATSYERKLAIAERSYTLLVEAGIDPSSIIFDPNV
                     LAIGTGIAEHDAYAKATIDATRELKKRYPRSYISGGISNLSFAFRGNDPLRSALHEVF
                     LELARFDLAILNPASRNQSTLDEQTRKIVRKALLAGDSSQLLNLALAEQAKEKIQNNR
                     ADLTPEELLARAIIEGELLGLEEVLDSLTHLEPLFIIDSILMEAMKTVGARFGEGKMF
                     LSQVVRSARAMKNAVDRLQPRLEAQSAEKKSSSGTRKAVLATVKGDVHDIGKNIVALV
                     LSCNNFEVIDLGVMVDEETILNAVKEHDPDLVGLSGLITPSLKEMEKVISLFEAEGLQ
                     LPVFVGGATTSEVHTALKLAPLSRGAVIRTKDAGEMALTAVEVLGPQGQARLEAIRTQ
                     YEAIRMRYTQEAEPSRGSKEDLLEKQEQKQTGLKAKRYGVFVKDDFDLSELAKRISWR
                     MYVQGWGVPFEDEEAKRIIAEAKELLAREEIASSFERGMKVVYSMMKVKSDRHTISTG
                     RHVFHFLRDEGSGFSLPDMVAQEDAVGFFAASSALDLEAILDDDPVKTLTLKLLADRL
                     AEVLAQEAQSLIADLWTERPRSIIRPAIGYPSWSDHSEKKTLFELLEVKERIGISLSS
                     SYAMDPPSSVCGAVIGGEGVRYFTVKDVSRAQLERYARRKGVPDRELASFLKDMGY"
     gene            58535..59488
                     /gene="metF"
                     /locus_tag="PLF67_01210"
     CDS             58535..59488
                     /gene="metF"
                     /locus_tag="PLF67_01210"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOE88662.1"
                     /translation="MRVTEIIQASKKPLFTFELVPPLKGRTLEPLMETIRTLMEFDPS
                     YINVTNHQAEVVYIEREDGLLQRKTVRKRPGTTALAALIQHTFNVPTVAHLICGGMSR
                     EQIEDVLIELHFLGIENILALRGDPPAGEKRFIPMPDGHAYSSQLVGQIVDLNHGRYL
                     DDQYRDGEATRFSIGVAGYPEKHAEALNMTVDLANLKKKVDAGAEYIVTQMFFDNGKY
                     FSFVNACRSIGITVPIIPGLKPIASKRDLSLIPQTFGVDLPEALTSELEGVDSNAEIT
                     EIGVQWCIAQSKELYAAGVPSIHYYTLGKARNVARIVRAVY"
     gene            complement(59485..60147)
                     /gene="nth"
                     /locus_tag="PLF67_01215"
     CDS             complement(59485..60147)
                     /gene="nth"
                     /locus_tag="PLF67_01215"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOE88663.1"
                     /translation="MDEHYWDSLFHRFRFALEKQGTILPSVSLVAQEHDDPFRILIAT
                     VISLRTKDAVTLKASRSLFSRAKTPSGILDLTEEEIQELIYPSGFYKTKAKTIRSISR
                     MILEEFGGVVPDTQEELMRLPGVGVKTANLTLNLGFGIDAICVDCHVHRIANRLGWIA
                     TGTPEESEQALMKVMPRRFWIPLNELLVRYGQVICTPVSPFCSRCPEETVCPRVGVTK
                     TR"
     gene            60237..60974
                     /locus_tag="PLF67_01220"
     CDS             60237..60974
                     /locus_tag="PLF67_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOE88664.1"
                     /translation="MSKIEICLDSVESIIASEQGGADRVEFCSDLFEGGLTPSLGMFK
                     VAKGRTAIPINVMIRPRGGDFCYSDLEFEAMKEDARLFKEAGANALVFGILTPEGKID
                     KTRSRELIAIARPLPVTFHRAFDMTSDPFEAIEDLIELGVERVLTSGGEASVLEGADL
                     LARLVKEYGNRIVIMPGAGITKRNFEKIHSIIKADEYHLYLHSEQPSKMVHRKDHVYM
                     GGLLRQEEYLVQHTDQSRVEAMIALVR"
     gene            61019..62923
                     /gene="aroF"
                     /locus_tag="PLF67_01225"
     CDS             61019..62923
                     /gene="aroF"
                     /locus_tag="PLF67_01225"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE88665.1"
                     /translation="MILILKKQITEAQKHAIREFLSDKGYTVKEIIGQEETVFGAVGQ
                     SSVDIRQVQLLEGVASVVPISKPYKLASREFKKEDTIVSVGKVKVGGNRIAIIAGPCA
                     VESREQIMAVARSVHDAGAVILRGGAFKPRTSPYAFQGLGEEGLKYLREAGDAYGMPI
                     TSEIVSPSDVNMMAKYVDMFQIGARNMQNFELLKAVGKTKMPVLLKRGLSATIEEWLM
                     AAEYLMASGTDQVILCERGIRTYERATRNTLDISAVPVIQKLTHLPIIGDPSHATGMR
                     DMVGSMSLGLVASGASGLMVEVHTDPDKAFSDGPQSLYPSQFEKLMRDLDALSGVLGK
                     SMERPVKVEGPKTGETEKKKISSPVVAFQGERGAYSEMAIRRAFEEDTEVLPCPSFTE
                     LFNAVREGRALYGMVPIENTLGGTISENLDQLERNDEIEVVGEQQIRIVHNLIGLEGT
                     KLETITHIYSHPQGLAQCTRFLTETMAHAQAVPFFDTAGAVAYVKRENDPSKAAIAGE
                     VAAHVYEMAILAEGIESHARNYTRFYVISRKENAEAFKTLRPINRASLLFTVGDRPGA
                     LFEALLILAKHQLNMKRLESRPIPGKPWEYSFFVETELGDNGAFEAALAELKETCLSL
                     RVLGTYGSNR"
     gene            62907..63428
                     /locus_tag="PLF67_01230"
     CDS             62907..63428
                     /locus_tag="PLF67_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOE88666.1"
                     /translation="MAQTGEFTKETLFLGVLAASENLLNEALNSLIERYGPIKKGSEA
                     TPFTFTDYYNEEMGGVPLRSYHIFEEAVDPSRLAEIKTTTNEVEHAFSIEGKRRVNLD
                     PGLLTLGSLVLATTKNRSHRIALNRGIYAELTLIYQDHSYRALPWTYADYKSRETIML
                     LNSWRSSLKQSKG"
     gene            63444..64172
                     /locus_tag="PLF67_01235"
     CDS             63444..64172
                     /locus_tag="PLF67_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88667.1"
                     /translation="MRLRCIIFISLLLFTSCDHNPLAERVVSVSIGEEHPWEEASYLP
                     LWYTLVYTTKNGNKKMHLSSGVRQARIVVDRLGSVAICAYPLGGFAPLGGFVQAGRSD
                     RVVLTRKDGPLAELLVEGNLLNAEAIASLNMDLLAALVGEAVNLDGSALLVDLLSGIT
                     PKKSLQRLERTWYTLSGIPDGYWVCENPSQSSFWIHFGKEVPLLLDHGATCWMNRERS
                     LILTLVSDNTTKMVFSALGDAPRW"
     gene            64273..64671
                     /locus_tag="PLF67_01240"
     CDS             64273..64671
                     /locus_tag="PLF67_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88668.1"
                     /translation="MVVVLLAGGVLFGAYIRDDLRYEGFFFHASEEPRTNDESTTIAI
                     GEIIVIVNRTSAGRISIWTTDLSDGRISHSYTICTSSGLVCAGKDTFHYQQNEDSPHI
                     EIGTLWAILPVSYIALEGFHSSIILELVLH"
BASE COUNT        14975 a        17566 c        17783 g        14416 t
ORIGIN      
        1 gccttcatat ccggctgtga cggcttatac gggtacaaga gaacgacagc taaaaaatca
       61 gaagaaggat tgcccaaccc cagatgggga acgtagcaca aaccaatgcg agaatcgtga
      121 ccttgcgatc cgtcttttta tccaaaccga acacgttcgc acctcctctc atgctgagca
      181 aaaagatgtt ggcaggagcc ttgtcttccg cttggcagga gccttgcctt ccgcttcaac
      241 acgccggtgt gcttatgata ccatggaaat gtcgccaatc ctacctatgg tatctcctta
      301 gaacttttga tttttatttt ggagagcttg ggtaggagtg gcgcatttca atccgttgcc
      361 aacaagcctt tttcaaagga gtgtgatatg aaaaaattct tgatgatcgt acgaaacatc
      421 gtgatcatca caatcgcgat gacgatcctc attgcaagta ttcgaaaaat caacgatatc
      481 cgatacagac cgaaaggcta cgacccaagc aaagcgtaca atgcaaagga cctttcgcga
      541 tacgaaaccg acatcgacgg tgtgcaggtc agtcgcatca tcggcgatta catgaacggg
      601 tttcgattgt tgcccgaaca caagacgcac aaaggcgtgc tcgtaacgtt cggcggttcg
      661 gaaggatcgc cgagttatga attcgcggag ctgtttgcaa aagaaggcta tgaagtgctc
      721 gcattgttct ttttcggcat ggacaaccaa cagcctgaac tcgtcaacgt ccccttggat
      781 tttttcaagg aagtgctggc gtacatcgat gagaacgtcg atccggacag cgatgaaaag
      841 acaccggtca ccgtatacgg agcatcgaag ggagcggaac tcgcactcaa cctcgcagtc
      901 atctatcccg agatcgacaa catcgtcatc atggcacccg gggcatggag tttcatgggc
      961 ttgccgaaac aatacagtca gaagatgtat gcttcatgga ccctcgacgg aaaggaggtc
     1021 ccctacatcg atatgtcaaa aggcgacctg aaaaccgtcg ccaatctgtt cttggacttt
     1081 ctcttgaaca gaccgatcga ttatcgaccg ggatatgaaa gcgcatcgat gaacgatccg
     1141 aacagcgaac tcgcacggat caaagttgaa aacacaaaag cgaacatcct catctttgca
     1201 ggcgacgacg acaaaatgtg gcacagcgaa gtgtcggcaa aaatcattca gaaacaccga
     1261 ccgaaacata ccgaggtgca tatctttgaa ggagccggcc acatcttcgc ccttggaaag
     1321 tacatctaca gcccgggcat gatcctggaa atgggcggca gcgcggaagc aaacaaaaaa
     1381 gccgccgaag caagctataa gattttcatg gagaacatgg cggaatggca tgggaaacag
     1441 gcaggtgacg gaatggatta ataccctgcc tatcggcaag attggtccct ctcgtttccc
     1501 cttccgtagc tttcaattca acctataagt taaattttca agcatttgtt ttacttatag
     1561 ataagattag acctctaagt aaaagttctt ttcacctagt gattgttttt ttgttttact
     1621 aatcgccgta catggtgttg tctttcttct tcttgatttg tttggaagag agatatggcc
     1681 tttcataaaa agcgctcatc ctctttgccc actggattgt tttccaatgc tttggcctga
     1741 agtggattaa aaactccttc gcacctgatt cgtcccgctt ctctttttat tacggatgga
     1801 agcgctcaag gcatggatcg gtcagggcgg cctgaagagc ggaaagtggt tgaacgaccc
     1861 cactcttcat tgaccgttga gtcttctctt tctattatta aggaaagagg atcatgccat
     1921 gagccaaaga accgtattga taaacggtac cgttgtaacg ggatatggaa aactgaagga
     1981 ttgtggagtc tatataaacg aaaacgggaa gatcggagac atcttcaaca tgtcccgctt
     2041 caacaacaag cactttcccc cggataccac gattctcgat gcggccggag gatacatcac
     2101 ccccggtatg atcgacagcc acctccacgg aatcggcggc ttcggaacgg aagacagcga
     2161 tccccactcg atccttcaaa tgagtgaacg cttggcggac gtcggagtat cggcgttcat
     2221 gccgacggtc tataccaaca cccttgatga gatgatccgc tcgatcaaag cgatcagcga
     2281 cgcgatgggt caagagaagg gggccaagat cctcggcatc aacgtggaag gtcccttcat
     2341 ctcgatgaag cggatcggcg cccagaatcc ggaaggggtc cttccggtgg atctggcgat
     2401 tttcaataag atcatcgatg ccggcgaagg caacatcatc tgcatgacgg tagcccccga
     2461 gctcaaagga atgcgtgaat tggccctctt ggccagggag cggggcatcg ttcttcttgc
     2521 cggacacacc gacgccagct atgagaacat cctcgagggg atgcaggtgg gaattttgca
     2581 ctccacccat ttcttcaatg cgatgagccg tcttcaccac cgggatccgg gaaccgtcgg
     2641 ggcgatcctc atcgagaagg acatgcagtg cgagatcatt cccgacggag tccacgttca
     2701 tcctcaactg gtaaagatgc tcctcaggga caagccgctg gataatatcg tgatgattac
     2761 cgattccctt aaaccgacgc gtcaacgggg gggaaacctg accgcaaacg gagttcccgc
     2821 aacgcttggg ccgaacggag cgtttgtcgc aaaggatgac cccgatctct tcatcggcag
     2881 cggactgacg atgcttcagg gggtacgaaa cgtcatcgac tgggaaattc ccattgaaca
     2941 tgcgatccag atgagctcga cgaaccccgc ccggatctat ggcctctcga agatgggaat
     3001 gctcataccg ggcaatatcg ccgatattct cgtgctcgac aagaatctgc agatgaaagg
     3061 cctcttcatc gacgggaacc tcatccgaga ccgattcgcc taataaacca aacagggagt
     3121 agttcatgca agacgcagta cggggccttg agccatctgc cctctggtcc tatttttccg
     3181 acctttccgc gattccccgc gaatcgggaa atgaagaagg ggtacgccaa taccttcttt
     3241 tattcgcaga aaaacacaac ctcaccgcga tcgtggaaga ggttggaaac gtcatcataa
     3301 aaaagccggc ggccaagggg tttgaagacc gcccttcggt ggctcttcaa ggccacatgg
     3361 acatggtatg cgtcaaaacg gagggttcaa cccatgactt tgcaaccgac ccgatccgat
     3421 tggtccgcga cggcgactgg attaaagcca aagacacttc ccttggagcg gacaacggaa
     3481 tcgccgtcgc cctgattctg gacatcctcg ccgatgcgga tgccaagcac ggtccgcttg
     3541 aaggaatctt caccgttgcc gaagaaaccg ggctgaccgg agcgttcggg attgaagaga
     3601 agaacatcga cagtcggctc ttgatcaacc tcgacagcga ggaagagggg gtgttctaca
     3661 tcggatgcgc cggaggagtc ggaaccaatg cgtacatgga tgtcctctgg gaagagaccc
     3721 cctcccatca ccgggggttt gagctttccg tatccggcct gaaaggcggc cacagcggag
     3781 cggaaatcca cacccagcgg gccaacgcga tcaagctcgc cgcccggatc ctgacccggc
     3841 tggatgaata ccgggtcttc aaggcgaccg gcggctcgaa gcgcaacgtg atcccctcca
     3901 ccgccacgat cggtttttca attcccaaag aggatgaaga tcggctctac caagtagtgg
     3961 agacggttct caaagaagtc aaaggtgaat tcgaaatggc ggacccccat atcgccattg
     4021 atgtaacaaa ggtagagacg cctaaaagaa gcgtggtcgc aaataccctg tttgatgccc
     4081 tctatgccgc tcctcacggt gtagatgcaa tgagcatgtc gattgagggc atcgttgaga
     4141 cttccacgaa cctcgccatc ctctccttgg acgagaaggg gttttatgtg agcgccagtc
     4201 atcgctcttc aattctctcc gcccgcgatg atgtcgcccg gcgcttcgcc acgatcttca
     4261 agctggccgg agcgacgacg aaacacagcg gcgcctaccc ctcctggacc cccaacatcc
     4321 actctccgct tgccgccttg gtggctcggg cctggaagga gtatacgaat gaggatgcca
     4381 aggtgacggc gatccacgcc ggcctggagt gcggaatcat caacagccgg gtttcgggaa
     4441 tggattcggt ttccttcgga ccgaacatgg aggaagtcca ctccaccaag gagcgggtct
     4501 cgatctcctc gaccggacga atcagcgact ttacccgata tctattggag acgattcggt
     4561 aaggattaca tcgccttgat gatcctggcc agttctttcg cccgggcaaa cgctttgtct
     4621 ttgtcttcct gcgtcggcga gccttggaac tcgatcgctt cggcgacgca ttcaagcttc
     4681 atcgcggtga cgaactcgtc gtattgcttc tgagctccgc cggaccaccc ataggaaccg
     4741 aagcgcagcg tttttcttcc tttgacgtgg ctgcgggcaa ggatgtccag cacgtgatac
     4801 gtcggcggga acatcttgta ttcatatgtg ggcattccga cgatgagccc ttcgcttcgc
     4861 cagaccttct ccagcacgaa ggattcatgg gtctggggaa cctgaaggac gtgaatcggc
     4921 agcccttcgc tctcaacacc ggcgatgatc gattccaaca gcgcctgggt gtttccatac
     4981 atgctcgacc acaccagcgc aacctctttt tcccgcggcc cgttgaggta ggaagcgagt
     5041 tttatgtagt ggttgatgat cgcttccgga tgggagcgcc agacgactcc gtggctcgga
     5101 gcgaccatcg ctatcttcag acccttcagt ttttcgatcc cccgcaacac gaagggggag
     5161 aaggaagaga cgatatttgc atagtagcgc tccgtttcgc tgtgcaggtg agccatcgta
     5221 gcttcatcaa gctcgtcgtc gaagcagaaa tcatatcgac cgaacgatcc gaacgcatcg
     5281 cacgagaaaa ggatttcatc ctcctgaagg taggtcatca tcgtctccgg ccagtggatg
     5341 ttcggggtaa ggtggaagac aagcgtcttt cccccgaggt cgagggtctg tccgtcggca
     5401 accgcctgaa cattctcttc gatcttgtag aacgctttga tgaggggaac cgccttcggc
     5461 gaacaaagga tttggacatc gggatagcgt ttcaccacct cccgaagggc gccggtatga
     5521 tccggttcca tgtggttgat gatcagaaag tccaaagtgt tcgtcgttac ggaaagctcg
     5581 tcaagctgcc tgttgatgac atcaagcgcc ccgtcccaat ccttgaccac gtcgatgagc
     5641 gcagtggatt tgctgccctt cacgacatat gagttgagca tcacaccgtc ggggatcggc
     5701 caaatgcctt caaataaatc acggtttccg attttcgctg cgacgcgata tacatgatca
     5761 gttaacagat cgggtttcat ctcaacaatc ctcctgctat gggactatac cttaaagagt
     5821 tggtggatgt atatcagtaa atcagctgac tcgcccgttg catggcgtac atccgctttt
     5881 gccggatctt ataatcactc atgtgggttt gaaggagtgc tttgaactct ccggagtggt
     5941 tgaagtggac caagtgtgcc acttcatgaa gaacaagata ggaaagctcc tcctcgccga
     6001 gcgcctgaga acggagagag aaattcaacc gcccctcttt ggagcagctt ccccagcgct
     6061 tttttgaatc acggatcgtg aacgaaggag ctgatactcc gagggtccgg ctccactgct
     6121 ccacaagggg cccgatccgt cttctcgcct ccgctcgata aagtgttttg attgcgtgaa
     6181 gaatccgccc cttgtcttgg atgtttcggg cggaaacgac gagaagatca tcttctatgt
     6241 atgcccgata atcatttcct gttcgaagtt ggagagtgac cattcctccc gcatagggga
     6301 accgatcccc gtcctcatag gtatgagcgt cccgcacttc ttcatcgatt cgattgagga
     6361 gatgctccct gtgttcggtg atcagctctt caataacccg cctgcttacc cgtctcggag
     6421 cccggatcgc caggattcca tccggaccga cccggatcgt gatccggcgg ttcgcccggg
     6481 aggtgagcag cgtgtaccgg atcgttccta ctttaccggt gcttttcatt ttgttagagt
     6541 agcctcaatg agcatgatga acctctccct ccgccaaggc ctagcgtacc aaaaactccc
     6601 ctgtgaggga agttccgccg aggatgcata tcgggcattg atctcgttcc ttgatcaggc
     6661 tcccgccggc agtgaaggag tccttcttct ctcctttgag atgaatgtcc tcttccttgg
     6721 aacgagcgct cccccggatg aggaaacgtt gaaaaagatt gctaaggcgg agaagctcga
     6781 tccggcggag ggcgatcatg ttcttgaacc gggacactac cgcttcattc agatccccct
     6841 gcccgcttcg atcgaagagc tccccttgga gaacctcgct ttggacgagg gtgatctgct
     6901 ctatcttcga atcctgaagg aaggaagctt tgccttggtc gctcagctgt ggatcagaag
     6961 aagagcggaa tgagaaccgg ggcgcagagg ctcgtagccg ccccatgata gatcgatccg
     7021 atcgtggtcg ccgaaccgaa atgcttggaa agcagcacga gcagcacatc catcgtcgtg
     7081 gcccctccgc aggagaccgc cggatagtag taccgttttc ccaatcgtcc gaaaaggggg
     7141 ataaggaaga acgagaagct ttcgcgcatc aggttggaga gaaaagatat tgagcccaaa
     7201 agaggaaatc ccagatcact gatgaggatt cccgagagcg aataccatcc gaacccgctg
     7261 agcattccgg cggcttcccg aaaggagaag gaggtcgcaa ggctcgctcc gaacgccccc
     7321 agaagggtcc ctgcgatcgt gtagaccggc aagagaaaga cgctcgggtc gcggagcacc
     7381 gttttgaagg agacggaacg ctgaatcagg ttcatcccca cgaaaaagag catcacatag
     7441 agcagatacg acacatagga ggatttgaac caatcaaaga aggtgctttt cattccgaaa
     7501 agcactccga gaataacgat tgaaatcaga aacagcggtt ctttcaaaag atcccacaca
     7561 tagcggagca gggagccttc ctttcgccgg gcgacggcga tcgttgtatc aagatccttg
     7621 ggcagaaaca ggcgggaccc ggcaagggag acgacggtcg agccggccag accgaagagg
     7681 gtcagaacca gcgcgctgag accgatacgc cccagttcgc cggcgatgtt ctcgatccgg
     7741 ccggtgttga tccccatgaa gaacaacaat acccacacca acaccgtctg cccgatcgca
     7801 ctgaatcgaa ccagtcgctc ctttccgatc gctttggaaa acaggctccc gagaatgagc
     7861 gccacggtga gctttgccag atagatgagg gtgtccatat aaaaaagact ctagctgaaa
     7921 gcgaacggga atgtcaaatt cccttgttta ataccgccac cttcggtata ttagcgcata
     7981 tgaccgtctt ggagatcagc gacacccgcc gcctcatcac cctcgccgat ggaagaagga
     8041 tccatctcat cggaaccgcc cacgtctcaa agcacagcgt ggatgaggtc gccgaggcga
     8101 ttgaaaccga acagcccgat catatctgcc ttgaattgga tgaaggaagg atgaagaaca
     8161 aggaagagga acgctcctgg tcgaacatgg atatcaagaa agtcctcaag gagggcaaag
     8221 ggttcctcct gcttgccaac atggccctgg cctccttcca aaaacggatg ggggacgaga
     8281 cggagacgca gcccggggcg gagatcctcg gagcggcgca cctggccaaa gaaaaagaaa
     8341 tccccttctc cctctgcgac cgggagattc aggtaacctt caaacgggct tggcggatga
     8401 gcaatctctg gaataaagcc aaactgatcg cgacaatcat cagtgcggtc ttctcaaacg
     8461 agaagctcag cgaagaggat cttgaagatt tgaagaaagc ggacgtgatg cagcagatga
     8521 tggacgaaat ggcagccgag ctgcccgctg tgaagtcggt tctgatcgac gaacgcgacc
     8581 gctaccttgc cacgaagatc tatcttgccc ccggtgagaa caagctggcg gtcatcggcg
     8641 ctgggcacat gagcggaatc gtcaagacgt tggaagccct cgactcaaac cggctcgata
     8701 cggacgtctc ggagattacc gtcgttcccg actcctccag atcatccaaa gcgctttcct
     8761 gggtcttccc cgtcctgatc gtctccctcc tgctctacgg attcctctcc ttcggccgga
     8821 ccgaagggat caggatgatc gggctctggt tggcggtgaa catgtcgttt accgcgcttg
     8881 gggcgattct ggcgctggcc catccgataa acatcatcgt ttcggttctg gccagtccga
     8941 tcaccagcct tcatccggcg atcggggtgg gaatggtcag cggcctcttg caagccacgc
     9001 tccgcaaacc caaagtcaga gattttgaaa atctcaacga tgatgcgacg aagctgaagg
     9061 ggtggtatcg gaaccgggtc ctccacatcc ttgtggtgtt cctcctcacg agtcttggcg
     9121 gctcgatcgg aaatgtggtc gttttcccct ttctcttggc gcgattggcg taaaaattca
     9181 tcgcctccat ccccataatt tgatacactg cctccaatgg aggtgacaat ggattatttc
     9241 agtcaagcca acagcgcacc gctgtacata ctggtcgggg tggttctgct cttcgtcgca
     9301 atcagctgcg tgctctttat ccgaaaaagc tatcttgccg gcctcaagat gggaatggat
     9361 cgtagcgtcc tgagaaccac gatcacatca agcgccctgt tcaccgtgtt gccgtcgatc
     9421 tccatcctgc tgggggtcat cgccctttca ggatcgttgg gggtaccctc cgcctggctt
     9481 cgcctttcgg tggtggggaa cctctcctat gaagcgattg ccgccgaggc cgccgctcag
     9541 gcgatgggcg tttccctgaa agacgccgcc tcgctgacca ccggccactt ggtaacgatt
     9601 cttctggtga tgaccaccgg gatcagctgg gggatcatcg ctacgatctt cttcggaaaa
     9661 tcctactccc gtaaatcaac ccaattgctc aacaagtcga aaaagaaaga gggaaagagc
     9721 ttcgccgact gggcgatggt tgcgatgttc atcggcttct gcggaacgtt catcggttcc
     9781 tacgtcgccg aagcggtggt gttcaaacac ttccttccgc tcctcaccgc attggtcgcc
     9841 ggcttggtga tgagagggtg tgaatatctg gtgaaagaaa agcagctggc gtggatggag
     9901 aacttttccc tctccctttc gatgttgatc gcgatgaccg cagcggttct gcttgccctg
     9961 taggaggaac ggaatggaaa aatcgtttga ttatgaacag tacgacgact cgatccaccg
    10021 cctcggacgg atcacgaacg cgatcggaat cctctccctg ctggcaactc ctttcctgtt
    10081 tgcgttggtg ctcggagcct cgctttcctg gtccggattc gcaaagggcc ttctccgagt
    10141 cgccatcacc tatctgccga tcacgatcgt cgaatttttg atctatgtgc cgatcctcgg
    10201 accgggagga tcctatctct ccttcatcac cggcaatatc atcaacatga agctgccgtg
    10261 tgcctacaac gcccgggaga tcgccaagac tcaggcggga actccggaag attcgatcat
    10321 cacgacgttg gcggtcggtg tttcatccct cgtgacgatg ttggtgatct tcatcggagt
    10381 cctcctgttg attcccctga cacccgtcct gaacaacccg accctccgtc ccgcgttcga
    10441 caacctgctt ccggcgcttt tcggagcgct tggcgcccag tacttcttca agagctggaa
    10501 ggtcagcgca attcctttga tcctgatgat cgccctttca atcgcgattc cggccctcat
    10561 cgcccagacg acgatcatga tgatccttat cggagcaatc gctatcgcca tcacctattt
    10621 cttccataag aaaggagccc tccaatgacc gaacgggaaa aacggtacgc ccggctgctc
    10681 tcgaagatga ttcaggtgga gacgatcagt gaaccgggaa tcatccatga agaaaagttc
    10741 atcaaaagcg aagaggttct cgcaaacctc tttcccaagg tgttctcgtc gtttgagaga
    10801 ctcccgatcg aaagcgggct tttgatgcgc ctgaagggaa agaccgaagg cgaagggttc
    10861 ctgctcatga gccaccagga cgtggtttcc gccgaagggg agtggacata tcctcccttc
    10921 agcggcgaaa tcgccgatgg caagatctgg ggccggggaa ccgtcgacac caagggagcg
    10981 ctgatgtgca tccttcaggc ggccgaggag ctgcttgaag aaaaatacat ccccgaagtc
    11041 gatttctaca tcgcttcttc atggaacgaa gaggtcagcg gacccggagc tccgaacgct
    11101 gcacgatatc ttcacgacca tggcgttcgt ctccagcttc tgatggatga gggaggcatg
    11161 gtggtcgatg aaccggtcaa ggggatcaaa ggccgctttg cgatgatcgg aacggtcgaa
    11221 aaagggtatg gcgacatcat cgccatcgct cacggaaagg gcgggcatgc ctccgccccg
    11281 ccgaagaact ctccccttgc ccgtcttgcc ggctttatga acgaactgga gaaacatgac
    11341 ccgaataccg ttgaactcag cctgaccctg atggagatgt tccgtcggat gggaagcagg
    11401 gcggatggag ttctgggctt tgttcttcgg aacgcccggt tcctgaaacc gctgctcaaa
    11461 cggatcctgc cgaaaaaaag cccggttctc gccgcgatga tgaaaacgac gatggcgttc
    11521 acgatggccg agggatcgct cggccgaaat gttttgcctc aagtcgccaa ggtgaatatc
    11581 aacacccgct tcatccaaca tcagggagtc gaggagacga tggcgattct cgctcccatt
    11641 gcaaagaagt atgatatcga gctgaagatc gaagatcact gccgcgatct tcaagagccg
    11701 gtggatttca acggcaaagg attcaagatc gtcgaagaaa ccgtcaacga gctctttccc
    11761 ggcgtcgtgg cgacccccta tgtgatgacc ggcggaacgg atgcctatca ctaccgcatc
    11821 gtaacggaca atgcgattcg gttcgctccg ctctccatca gcaatgagca gcatgctacg
    11881 gtccacgccg ccgacgagaa catcgacata gcggtcttgc cgccggcggt cgacttttac
    11941 aaggcgatca taaaaaaggt gagataaaag caaaaacatg ctagaatgca cgccatgaat
    12001 acaagctcga cgtcctatac accgattgac cttccttccc ttgcgaagaa ggtcaatccc
    12061 aagcttgcaa aggtcatccc tcgctgcatg tatagaaagc tggagagact tcttcacgtc
    12121 aaagagatca acgagttctt tgcagctcat cacgatgatg atgcccggac gtttctcgat
    12181 gcggtggtct cccatctcga tctgcagatc gtcatcgaag ggcaggggat cgaagccctg
    12241 cacgctcttg aaggggaacg ggtaatcttc gcttccaacc acccctatgg aggaccggag
    12301 gcgatggccc tgttctccat cgtccatcgg ctctttcccg atgcgaagct ggtggctcaa
    12361 tccttcctga agttcatcaa gcccattgaa acggcgtgcg tgttcaacaa gaaagaagtg
    12421 aagaccctga tggaagcggc gaacaaccgc agcagcctga tcttctaccc tgccggatac
    12481 tgttcccgaa tcctttccaa cggcgaggtc ttcgactaca gctggcagtc ttcgttcgtg
    12541 cgaatcgccc gaaaacagcg gatgccgatt ctcatcatct acaccgacgg cgagctgagc
    12601 aggaaagtcc atcggtggac ccggttgcgg aagctcctca ggatcaagct ttcaatcgag
    12661 acgatcttcc tggtcgatga gatgttcaag ctgaaaggca agaccctcag gatggtcgtc
    12721 ggctccacga tcacgcccga gatgatcgac gaagcaaaga tgagtgatcg cctcttagcc
    12781 gacaagctgc gccaatattg ctacgagctg cgcaaggatc ccgagctcgt cttcgatccg
    12841 aacattgttc cgacactccc tcagctttaa gaacaaatac tatagttagg ggtaataact
    12901 aataggatct tcgatatatt caaccatata tctcagtttt taatatgttt aggtataacc
    12961 atttataatt gttaggctcg ctaattattg tatcaatgca tctctcggac cgcatcgagg
    13021 gcatcccgaa tcgttcgggc tccatattga gtcaaagaag attgtttgga ggccttacta
    13081 actaaggatc gggtaaagcc cagctctttg gccgtcttct cccggcgctg aatgaacccg
    13141 accggacgaa tctcccccga gaggctcagc tcgccgaagc tcaccaaatc ctttggcaga
    13201 ctcgtccggg taagcgccga atacagggcg agagcaagag ccagttcgac ggcgacctcc
    13261 cccaccttca tccctccgcc gacattcacg tagatgtcct gatcgctgag tctaaggccg
    13321 gcgtgccgtt ccagcacggc ggcgattcgg gtaacccggg cgctgtcgat ccggtcggag
    13381 aagattctgc tgaagccgct tttggcggga acggtaagag cttggacctc caccagaaag
    13441 gaacgggttc cttccgccac cgcagtatag gctactcccg gaggcagggg cccctccgct
    13501 cgatcggaaa gaaaaaatga ggcgggattc gctaccggat gaagaccttt ttcatccatc
    13561 gagaacaggc cgatctcatc gacgcttcca aagcggtttt tcgccgcccg aataaggcgg
    13621 attccggaag gcagttgatc gaagtacaga accgtatcaa caaggtgttc aatgaccttg
    13681 ggtccggcaa tcgtcccttc cttggtaacg tgggcgacaa agaataaact gatccccaat
    13741 cgcttagcca agctgaccag cgcgttggag cagtgtctga tctggttcac cgatcctgcc
    13801 ggcgaaggca gcgtatcact gctgagcgtt tggagggaat ctatcaccac cagtgaagct
    13861 tttgaacttg gcagaacctt ttcaaggact tccagtctgg tatcacagaa gatattgatc
    13921 ttgcttaggt gcaagccgag gcgctgggcc cgcattttca cctgcgaagg tgattcttct
    13981 ccgctcacgt agagcacctt ggaggtattc cgggtgcttt gaagaagctg gagcatcagc
    14041 gtggacttcc cgatccccgg ctcgcctccg atgagcaccg aggagccttt catcactcct
    14101 ccgccaagga cccggtccaa ttcgctgatg ccgctggcat agcgaaattg aggatctacg
    14161 acaacatcct ccaaccttac cggctcttca tccgtgctga gcggctttgc tcccttcgag
    14221 ggagtgatga tgagttcctc ttcaaaactg ttccatgagc cgcaatcggg gcagcgaccg
    14281 agccacttcg cttcagtcct gccgcaatta gagcatacga attgcagatc gccgcttctt
    14341 ttcgccacat caaaccgcct tcagaaggaa aggaggtgaa cctcgaagat caggtaggag
    14401 ttcgggggaa tgattcccgg atatcccatc tcgccgtagc caagctccgg tgggatgatg
    14461 agagtccgtt cttcacctgc gctcatcgtc acaagcgcct cattccatcc ctcgatcact
    14521 tctcctaccc gaaactccgt cggctcgccg cgttgaatcg acgagtcaaa gattcgaccg
    14581 tcgagcaggg agccttggta atggaccttc actcgggttc ccatcttcgg aaaacgctcc
    14641 ccatccccct tcttttttac tacataccta aggccggtct tcgtacgaat cgcttcagga
    14701 tatcgattct tcagttccgc ctccacgttc gcccgtgctt cttcgagaac tttttttcgc
    14761 ttattttccg attcaactat atatgatgta aatatttgct tcgtaacctt gaaggcttcg
    14821 gcatcactgc cgactcgaag aatttcaacc tttttgatcg tatcgccttg agcaattgaa
    14881 tcgacgacct tctgaccttc aacgacgtgc ccgaagaccg aatgtctgtt gtcaagccaa
    14941 ggggtttcta catgagtgat gaagaattgg ctcccattcg ttccggggcc gctgttcgcc
    15001 atcgaaagaa ttcccgctcg atcgtgcttc agggaaggat cgaattcatc accgaacttg
    15061 taccccggtc caccggttcc cgtccccaga gggcatccgc cctgaatcat gaagttcgcg
    15121 atcacccggt gaaacttcaa cccatcatag aagggtttat ccttattcaa acttccctcg
    15181 gcaaggccga cgaagtttgc aacggtcatc ggtgttttct tatattcaag ggcgagcagt
    15241 atatccccct tgctcgtatg cataacggca tagaggccgt ccttcagagt tttcgtatcc
    15301 atgaattcct tcctcaaaat aaatgctcat tgggaacgag gatctgatat attcgttcca
    15361 gttcttccgt gctggaaaag gggatttcaa tcctgccttt cttgatcgta cccttgattt
    15421 cgacttgggt gccgaacgcc atcaaaaagc tttcctcggc ggctagaatc tccagactct
    15481 tttccgcttc tttcttggat cgctttttct tttggtgcgc cactcgccta ccctggttga
    15541 actgagtggc catctcctcg gttgcccgaa cagataaatc tctttcaagt aatgttttat
    15601 acagaatttc ccgatcggcc gggtttacaa ccgagagaat cgccctggca tgtccggcgg
    15661 tgaacttgcc cttaagaagc tcatcctgca tcacggggtt gagctgaagc agtcggatgc
    15721 tgttgctgat cgtcgatcga ttcttcccca gtcgtcgggc aagctcttcc tgggtgattc
    15781 ctgcttcctg gatcaggtag gcgtacgcct gcgcttcctc gatcggattg agggattccc
    15841 gttggatatt ctcgatcagg gagatttcaa ggcgctgcat ctgcgttacg tctttcacca
    15901 gtacgggaat ggttttcaaa cccgccatct tggatgcccg gtatcgtcgt tccccggcta
    15961 caatcgaata ccggttcgga ccgatctctt cgaccaggat cggctgcaag accccttcac
    16021 gttttatcga ctgggcaagt tcctccaacg ttgcgttgtc aaactctttc cgaggctggt
    16081 ttgggttggg ccgaaccgca tcaagctcca cttccaaaac ccggtctccc ttctcttcct
    16141 ttttggctga gctcaaggcg ttgtccagca ctgaatcata ggagaaatcc tgcatcaatg
    16201 aaccgatgcc tttgcctaat ccaatctttc gtttactttc cacgtttaat gacctccttc
    16261 gccaaagctc gatacgcctt cgctccgttg caggagccgt catactgatt aatcggtaaa
    16321 ccatggctgg gggcctcggc caggcggata ttccgaggaa tcatcgtatc aaacaccaga
    16381 tcatcaaaat aggagacgat atcgtcgacg acatcccgtg agagacgggt tcgcttgctg
    16441 tacatcgtaa agagaattcc cagtacttcc agctccggat tcaatgattt tttaatattc
    16501 ccgatcgtat gcatcaaaag cgtcaaccct tccatcgcaa aatactcact ttgcatagga
    16561 atgatgattt catttgccca caccatcgca ttgatcgtca ccaggcccaa tgatggggga
    16621 caatcgatga tgatgtattc ccaattgcct tcaaggggtg aaaaagcatc cctaatgaaa
    16681 tattcccgcc gatcctgatt caccagctca atcgcaagcc cggccatatg gatattgctc
    16741 ccgatcgcaa agagattcct gacggccgtc tgttgaatca cttcacttag ctcggcgctc
    16801 ccggcaagca cttcatacac tccgggtttt cgagcgtcca aaccgacacc gctggtcaga
    16861 tttccttgcg aatcgatatc aatcaataag gttctctttc ccatttctgc caaggaagcg
    16921 ccgaggttga tcgcagaagt cgttttcccg actcctcctt tttgattcaa aaacaagatg
    16981 atctttgtct tcatgattga tactatactc aaatgtttcc aatctgtcac cgccttgacc
    17041 gttatctttg aacacggtat ctttctctta cccgatagga ggtgttccat gttggaagat
    17101 aaggtcaaac aagcaattca ggatattcgt cccgctttgc agaatgacgg tggagatatt
    17161 gagttcgtca gcctggcagg caaggaggtc acagttcgcc tcgtcggagc atgcgccggc
    17221 tgtccaatgt cacaaatgac tcttaaggga ggagtggagc gctatttgcg caacttcgtc
    17281 gatccccaac ttacggttgt caatgaacgg tagtccttat ctgttcatgt tctttgcgaa
    17341 gaacgtttca cgtgaaacat cgtgagacgt tttttaatgt ttcacgtgaa acattcctta
    17401 tcctataaac acgttcatta aaaaatatcc ctatattcct acacatagcg atttccattg
    17461 tcttcaacca cattcctcct tttctcctat ttaatcgttt gtctttttaa aacaattatg
    17521 ttgtacacta tttagtttcg tgatatagtg gttctgtaac gaacggaagg gtaggcgggg
    17581 agattggtcc atccatcatt ttcgaataat gttcaaatac ggtaacgttt atcctgtttg
    17641 tttgttttag gtatagagag gatcttcact ggggtgtagt gaagtcgaaa tgaagaaggg
    17701 gtgcgtttca cgtgaaacat ttattgggat cagtgcgaac ttatagcatc catatgtctc
    17761 ttgagcaatt caagagattt ttctgtatgg ttttgcccgg tttatttcaa aaacattctg
    17821 aacactccaa aacctgcatt taaaagcgta gtttaaaggt ttttaacccg gtttctataa
    17881 acagaaacag aatcgtgtga gcaatccacc catttgttgt atgcatatat cgctataagc
    17941 tatattctct tttcatcccg atagttccta taaaaaaccg cgccgacttg attgatgacg
    18001 cggtaatagt atgatttgaa gagactgatc agaacttagg cttcgtcatc atcctcgaca
    18061 tcgatatcac tgtcagcctg atcgccttcc tcgtcggggg tttccacttc agcttcatca
    18121 tcgcgatcgg ttgaggcgat cgcaacgatc gagtcatcat cccacttcat ccggacgacc
    18181 cggacgccgc cggcgtttct gccttgaatc gagattgtat cgacatggat tctcagtgtt
    18241 tggcccaaaa gcgtaacgca gacgacatct tccgtttcgc tgacgccaag cactccgatg
    18301 atctcgtcgt tctttctcag ccgaagaatc cgttgacctt gggttccccg gttatgcggg
    18361 atgaacgcat caaactccac ccgttttccc tgtccgttgg cggtgatcac gaggatatgt
    18421 ttcccttctt cgactttcac cattccggca agctggtcat catccaccaa gcggatgccg
    18481 cgcactcctc gggtcgctct tcccatcgct cggacctgat cgctttggaa acgaagagcc
    18541 cgggcgcctt tggtaacgaa catcacctca tcgccatctt ccaccagctc gcaggagagc
    18601 agttcatcac cctcatccaa gacaatcgcc caaatgcctc gcgatcgagc attgacgaag
    18661 ttatgaaggg ataccttctt cgttactcca tacagggtgg ccatcatcag gtactgctca
    18721 tcggtgaact ccttgaagca gatgatcgaa gttaccttct cttccgtttc aatttgaatg
    18781 aagttcttga tcgaaacgcc tttggcatat tttccagcct cgggaatatc aaagaccttg
    18841 gtgtaatacg ccttgcccat gttggttaca aacatgacgt aatcgtgggt tgaggcgaca
    18901 aacatgtggt cgatgaagtc gccgtcttga agcttcgcgc tgcgaacgcc cattccggcc
    18961 cgtccgcgtg cctcatactc ctcggtgggc acccgcttgg cgaagccctt gttggagatc
    19021 aggacgacga cgtcctcttc cttgatgaag tcggccatcg tcgcttggcc gagctcctcg
    19081 acggtgatct cggttcttct cttgtccctc ggtgcaagat tttgaggcag cgcattgatc
    19141 tccgcctgaa cgacacccat cagctttcct tcatcggaca gcagctcgtt gtagtaggcg
    19201 atcttcgcct ccagatcttc aagctcttca agaatcttgg aagactcaag gtggctgagc
    19261 cggccgaggc gcatgtcgat gatcgcctgg gcttgaatct catcaagggc gaaggcggcg
    19321 atcagccgct ggctcgcgat actgttgtca ggcgaatctt tgatgatttg aatcacttca
    19381 tcgatatgtt caagaccgat cttaagcccg cggaggatat gggcgcgctc ctgagctttg
    19441 cgaaggtcgt aggtcgtccg cctcgttacg acatctttgc gatgcttcac atagtagtgg
    19501 agcatatcct tcaatccaag aagttggggc cttccatcga ccaaggcgag attgttgacg
    19561 ttgaagttcg attgcagagc ggtccgctgg aagagctggt tcaagacgac catcacttca
    19621 gcgcccatct tcagttcgat gacgatccgg atcccgttgc ggtcggattc atcccgaatc
    19681 gtgctgatca tgggaatcga gccgtctttc cgaagatcgt cgatcttctt caccagatcc
    19741 gctttgttca cctgataggg gatttccgtg aagacaatga catcgcctcc gcgggcggac
    19801 tcttcgatct cgtacttccc tcgggtgatg attcgtcccc gacccgttga aaacgcgtcc
    19861 acgatccccc tcatcccgca aatcacccct cccgacggaa agtccggccc cttgatgtgc
    19921 tccatgagct ccatcaacgt tatctcggga tcttcaatca acgcattggc ggcggcacag
    19981 atctcctgaa ggttgtgcgg agccatgttc gtcgccatgc cgactgcgat tccgctcgac
    20041 ccgttggcca gaaggaaggg aaacgcgccg ggaaggacgg agggctcggt cagcgaatca
    20101 tcatagttcg gcttgaagtc gacggtttcc ttttggatgt cgctgagcat ttcttcaccg
    20161 atgcggctca tcttcgcttc ggtataccgc atcgccgccg cagggtcgcc gtcgatcgat
    20221 ccgaagttgc cttgcggatt cactaccgga tatcgaagcg agaaatcctg agcgagccgg
    20281 accagagcat catacacgga agcatcaccg tgggggtgat acttacccag gacgtctccg
    20341 acgatgcggg cgcttttctt ataggaggag ttggccctga ggcccatctc atacatatca
    20401 tagagaatgc gccgatgaac cggcttcagt ccatcgcgca catcgggcag ggcccgactg
    20461 acgatcacgc tcatcgcgta gttgagatag ctcgtgcgca tctcgcttgc gacatctact
    20521 ttaatcgtcc tgttttccat cgtatccaca attctatcct tgtttacaca tcaagattgg
    20581 agacatacac cgcattcgca tcgatgaacg cccggcgggg ctcgacctct tcgcccatca
    20641 acatcgagaa cacccggtcc gcttcctcgg catcgccgag ggtcacctgg ctgatcaggc
    20701 gggtctcggg attcatcgtc gtctcccaca gctgctcggg gttcatctct ccaaggccct
    20761 tgtagcgctg gatggaggcc tttgccgcat caggaccggc ttcttcaaga atcctgatcc
    20821 gagcttcttc atcatacgcg taggaaacat ttttcccgat cgtgatctta tacagcggcg
    20881 gcatcgcaaa atagacatat cccgcttcga tcaacggccg catatagcgg aagaagaacg
    20941 tcaagagaag cgttcggata tgcgaaccgt cgacatcggc gtccgccatg atgatgactt
    21001 tatgatatcg ggctttggtg atgtcgaacg ttacatcgct gtcgagatcc tcgtcttttc
    21061 ccatgttgtt gtagcgggtg agccccgctc cgatcgtgct gatcaccggc tgaagcttgt
    21121 cgttgcccaa aactcggtat tcttgagctt tttccacgtt cagcatcttg ccccacagcg
    21181 gcaagatcgc ctggaagcgc cgatccctgc cttgcttggc ggatcctccg gcggagtctc
    21241 cctcgacgat gaagacttca cagagcgccg gatccttctc ggagcagtcc gccagtttgc
    21301 cgggaagacc tcctccttcg cttttctttc ggatctgttc ccgcgctttt cgagcggcga
    21361 tccgtccgag cgccgcgttg atgatcttcc ccaagatcag ttcggcgtgg gccgggaact
    21421 cgtcaaagta gttgccgagc ttttcataga ccagggaaaa gaccacgccg gtaacgacgc
    21481 tgttgcccag cttcatcttc gtctgacctt caaattgagg ctcgggaatc ttgatcgaca
    21541 cgatcgccgt gagcccttcg gagatatcgc tcggctccag acgatcctca taccgcttca
    21601 gaagcttctg gttcttctgc agctgggcgt tgatcacccg ggaaagcccg gttcgaaagc
    21661 cggtcaggtg cgttccaccc tctttcgtgt tgatattgtt cacgaaggtc agaaccttct
    21721 catcgtattt gtcgttgtat tggagggcga tctcaacttt gatgttgtcc cgctccccct
    21781 caatggagat cggcgtttcg ccgagggtgc gcttgtattc attaagatag ctaacaaact
    21841 caacgatacc cccttccgag tgaaaggttt gctttttcgg ctcttcaaag cgggtatcgg
    21901 ataccgttat ggtaattcct ttgtttaaaa aggagagttc ccggaaccgg gttacaaggg
    21961 tttcatagct gaatgcattc tcttccagga tcgtgtagtc cggtgaaaac gtaaccttcg
    22021 ttccggtctc gtcggtatcg ccgatccgct gaacctcact ggtgggattg ccctgactgt
    22081 agagctggcg gaagattccg ccatcccgct tcacgatcac ttccatccag atgctgaggg
    22141 cgttcacaca tgaaacaccg acgccatgaa gccctccgct gaccttgtag gagttcttgt
    22201 cgaacttccc tccggcgtgg agctgcgtca tcgcgatctc tagcgaactc ttcttttcaa
    22261 tcgggtggat ggcgacggga attccccgtc catcatcttc gacggtacag aactgccgtc
    22321 catcttcatt tcgggaaagt gtgacggtaa tcgtgtcaca atagcccgcc atcgcttcat
    22381 cgatcgagtt gtccacgacc tcgtagacga ggtggtgaag gccgtcaatt cccgttgaac
    22441 cgatatacat ccccggtcgt tttctgacag cttcaagccc tttgaggact tgaatcgagg
    22501 aagcggaata ctgatccggg ggcttcactg cacccgttgc ttttgcaccc gcggaaaggt
    22561 cagcattccc attggaaaaa ctcccatcat tcattggtat atccttatgt aataataaaa
    22621 aaatctataa attccattcg ataaattgta tcagaaatgg caaagtgttg caagcggacc
    22681 ccgaagtttt tcacaaaata tatatacttt caagataatg atttatactc acatgaaact
    22741 gctcatatgg agaatgcttg caccgtcgtt cactcaacgg tagaataggt ggtatgagcg
    22801 accaacaaac atcatattat gagttctggc tggagacggc aaagcggatt caggaaaccc
    22861 ttccgcctca ggaattttcc acttggttca gccgaatctc ctatctctcc tccaaaaaag
    22921 gagaggtcac cctcgcaggt cccagccaat tcgtcctcga taccgtaacg acccgttatc
    22981 gatcgatcat cgcgaacacc ctcagtgaac ttaccggtga ggagatcgcc gtcgagtttg
    23041 tcgtctccga caaaggaaag aaagccgtcc ggccgatgga cggcgatgaa accgctgaag
    23101 aagctcgaaa agagaaaaaa gccgatgatc ttgccaagaa gatttccaag cggaaaagcg
    23161 aggcgaatct gaatccttcc tatgaattcg aatccttcgt gcttggcgac aactccgcct
    23221 ttgcgtacaa cgcgagtctg gcgatcgcaa aaaatccggg ttcgagctac aacccctgtt
    23281 tgatctatgg cgatgtgggc ctcgggaaga cccatctgct cagctcgatc ggaaactata
    23341 tcctcgatca tacccccgaa tgcaaggtga tgtacgtcac cgccgaaaca ttcaccaatg
    23401 aattcatcga gtcgatcgga agccagaaaa cccagcagtt caagaacaag ttcagaaacg
    23461 tcgatgttct gttgatcgat gacatccact ttcttcaggg gaaggattcc acccaggaag
    23521 agctcttcca caccttcaac aacctctatg aagcaaagaa gcagatggtg ttcacctgcg
    23581 accgccctat cagcgaattg aagaacatca ccgatcgcct ttcctcccgg ttcgagcggg
    23641 gattgaatgt cgatcttcaa ccgccaaact atgaaacgag ggtcgcaatc cttaagaaga
    23701 agcttcaaga acatggttca tccctctcag aggatatcat caactatatc gccttcaatg
    23761 tcgtaaccaa cgttcgagac cttgaatcct cgctgacgaa gcttgtcgcc tacatggacc
    23821 tgatcgggga agagttgacg ttcgagaaag caaagcagct tctggggatt cttcccgcga
    23881 tggccaacat cacccgggac actctatcca tcggcgagat catcaagact gtcggtgact
    23941 atttcaacgt ctcgacgttc gagatgaaag ggaagaagaa aaacaagagc ctggtccagc
    24001 caaggcagat agcgatgtac ctcgcccggc aaatcaccga cttttctaca acggagatcg
    24061 ggatggaatt cggcggccga gatcatacca cggtgatgta tgcgaacaac cgggtcgaat
    24121 cgctgatgaa aggagacgag gcgtacgcgg cgaccgtcaa taaactgcgt cgtcaattga
    24181 cttcttcctg agccgtatat cctggggata acctcttggg aaaatgtgga caacgcttca
    24241 agcctgtgga cagcttatta ttgcttgtgg atgaactgtg gatatctttt gaacctattc
    24301 acaagctgga aaaatttaat aggttctttt acaatgaatt gtgtcagtta tccacagttt
    24361 ctgtgctatt attactatta ttactgatta ataataaaat aggagtagag taagatgaag
    24421 ttcatctcaa caaaagaagc gattctttca gaaatcatat acacggtgga tttcaccact
    24481 caaaagaata gtcttgcgat caccagcaac gtataccttc agactctcgg tgatcagctg
    24541 ttgattaaag ccaccgacgg caaagtggga ttttccactt caatcaccgt cgatgttcag
    24601 gaagaaggaa aagctcttgt tcttcaggaa aaattgttag agattcttcg tgttctaccg
    24661 gatggagata tcaacttctc gatgaaggac ggcttcttca cgatcgtacc cgtcggtcat
    24721 gggattgatt tcaagatccg aatgattgaa gccgatcagt ttccggctct tgaaacgatt
    24781 gacaatgatc gattcttcag catcgcccaa agctctttcc ttgagatgag cgaacaagtc
    24841 attttcgccg tcagcgatga tacatctcgg tactttctct gcggagtcta tcttgaacga
    24901 agcgaaggcg gaataacgat ggtggcgacc gacgggagaa gactttcgat cgtcgagcgg
    24961 gtgttcgaag aaaaactgcc ctccttcgca ccgattatcg tgttgccgaa gtttttccag
    25021 atccttcgaa agattggcac cggtgagggg gcggtcgagc ttgcaatcac cgagtcgatt
    25081 ctctttgccc gaattggagg acggacgttc tatatcacgt tgatcaggga gcagtatccg
    25141 aacttccgcc gggtcattcc cgagcagcag agctaccggt gcacgatgaa aattcaggat
    25201 atggaagatg cactgaggcg ggtggcgatc cttatcgagt caaaggcgaa acgcttgttc
    25261 ctggatatca gtgaaggcgg cgtccttctg acgagtgatg tcagtgaaag cggagaagcc
    25321 aaggagatcg ttccctgcca gtatgaagga ccggattgca agatctcttt gaactacacc
    25381 tatcttctca ctccgcttag ggtgatggag ggggaatatt tctcgatcaa cttcaccgag
    25441 ccgaaccgat cgatgacggt gaagccggaa agcgggcgtg actacatgca tatcatcatg
    25501 ccgatgcagc ttaatccttg atgcgctttt gtacgattac gacgaaccaa ttcagaaatc
    25561 tcaaggagga acccttggaa gtggacgctt ccaaggttct ccttatcggc cccaacggtc
    25621 aaggtaagac gaatctgctt gaagcgctgt acacgctgtg ttacggctct tcctttcgaa
    25681 cgacccagat caaggaactg gcaacgtatg gcttcaagga ttttcgaatc gaaggaacgt
    25741 atcgggatga acggaatctc cttcataccc tcaccctcac gttcaaggac aataagcggg
    25801 tcatcacctt ggacggtacg gtaatccgtg accggaaaga actgatctac aatattccga
    25861 cgatcgtctt cagccacgat gacatctttt ttgtcaaagg ggagccggaa tatcgacgga
    25921 agttttttga tcagacgatg agcatgtata atccgctctt cttcgatgat caccgccggt
    25981 atcgccagat tctgcgccaa cgaaaccagg ctatcaagga tgagcagtac tccttgctgc
    26041 cgatctacga tcaacagctt gcccgccacg gaatagccat tcaacgggaa cggattcgtt
    26101 ccgtctacga gtttctacaa atctttccgg aaatctatca ggaggtctcc cggagcgaca
    26161 cccttctgga tatcgagtat cggccatcct gggaggagtg cgccaccgag gaggaagtcg
    26221 ttcactgcct cgaatcccat cgtcggaggg atatcaagct tcaaacgacg acaagcggga
    26281 tccatcgtga ccaattcatg atcaccgagg atggctaccc gttcgccaac aaagggtcga
    26341 cgggccagct gcgcttggct tcgctggcgc tccgggtcgc tcagatggcg tacttcacga
    26401 aaaaaacgga caaagaggcg gtcatccttg tcgatgatgt tcttttggag ttggatattc
    26461 caaagcgcga gcggtttctg accattcttt ccggctatag tcaggcgttt ttcacgtttc
    26521 tacctgaaga acggtatatc cgctcctttg aaacctccga tcggaaagtg tattatgtca
    26581 gtgaaggagc atttactctt ggccagtgat gaaaaacccc gcctcagcaa gggcgatgat
    26641 cccctgctcg gcaaagaagt cctgaacacc gtcctcaaaa cgcttgacat tgatcttgag
    26701 gaaccgagat acggccttac tgcaagctgg gaggagatcg ccggtcccga gctcttcccg
    26761 cacgtgaagg tggtggacat ccggcatggt tcgctggttc ttcgagcgga tcatccctca
    26821 tgggccaacc tcgtcatgct gcaaaaaaaa cggatcgtcc aggcggttcg gaagcgttat
    26881 ccgagccttg ggatcacaac cctttccgtg ctcggagctt gacggaacag agcgctctct
    26941 gtatactgca ccactcgggg agtgtccccc gcttgatatg aatgatccgc gttaagcgat
    27001 gtaaattgga gaattcccat gagttacaaa ggtaagagaa cctatcagcc cagccgggtt
    27061 aaaagaaaca gaaagttcgg gttccgcgct cgtatggcca caaagggagg aaggctcgtc
    27121 ctcgctcgcc gccgggcaaa gggtcgctac aagctttctg ttgcagatga gaaaaagcct
    27181 ttctaagagt gagattgtaa aacgtagatc ggaaatagat cgaatcttta aaaccgggaa
    27241 gaaaatttca agccggggca tgaacttgat tgtaacgacc aatgaccttc cgttcagccg
    27301 gatcgtggtc attcccgtcc gagggtatgg ttcgtcggta aagcgcaaca ggatccgtcg
    27361 acagatcaaa gagatctggc gcactgaaaa agatcgattc cttccgggct acgattttgc
    27421 atttgtcgta tacccaggaa gaaatgtaga ccatgctctt caaaggcgac atgtactcac
    27481 tctgtgcaag caagccgggg tgatgaatcc tccctgattt ctttgagtct acctattcct
    27541 gaacagcacc ttcttccgat agcaggagta gtcatggacc gaaacaccat attagcagtc
    27601 attctcagcg tgctcgtcat caccgtgggg atgacgattc aagcgacatt ttttgccccg
    27661 agtgcgcaag aagaggttgc cgaagaggct gccaacgtcg aagagatcta tcttcaaccg
    27721 gaggcctaca gcaaacttcc gagcaatttg gcgtggggaa ccgatctgcc gggttcgttg
    27781 atcgctcttg aaagtcccgc caactcctcc ccgtttgtct acgaaacgga catctttttg
    27841 atcaccttcg atcccaaagg cgcttcaatt tcatccttca agctgaagaa gcacctggac
    27901 aagggcgagc cggtggagct gctcttcggc ggttcgaatg ccaacaacgc cttcctcatg
    27961 tatgccggat ccgaccgcac tcaaccgatt gacgctccgt tcaactatcg gatcgccggt
    28021 aaccaagtaa tcttcaccca gacgttcgcg atcaagggcg aggatgttcc attcacgttg
    28081 acaaagacgt tcaccttcgg agagagcgag tatcttttcc aaatcgatgt gaacctcgtc
    28141 aacagcgaaa acaaggcgat tccccttgcc ttcgacaata cggcgtatac gatcggcttt
    28201 gagccgcaga tcgggccttc cttcaccagc atcgacaacc gctactccta ccgccgcttc
    28261 tacatcagag aggacggagc gaagaaaaaa gccacgatca cggtgaagaa cgggatctac
    28321 accacggaaa aacccctttc ctgggtcgcc ttgaccggca agtatttctc gatgatcgcg
    28381 attcccgatg cgaccaacta taccgttacg ctgagcgaag acacgaaaac cgatattccc
    28441 ctcgagtcga acgtgttctt cagccgaccg gcgattcgga gcgccgacca gacggatacc
    28501 tatcgcttct acatcggacc ccagctgaaa gcggacatgg ttccctacaa cgatcgggcg
    28561 agcaacagct tcgggctttc caacctgttc ctagaggata ccctcgattc ttcttcgtgg
    28621 ctggggtggc ttgaaacgat actgaagacg attctcaacc ttctctacaa ggtgattccc
    28681 aactatgggg tgggaatcat catcctgacc ttcctcttga agctgattct ccacccgatc
    28741 accaagaagg ggatggagtc caccgccaag atgagcgccc tcgggcctca gatggaggag
    28801 atcaaggcgc gctatccgga taaccccacc aagcagaacg aggagatggc ggagctctac
    28861 aagcgggaga aaatcaaccc gatgggcggc tgtctgccga tgctcctcca attcccgatt
    28921 ctcatcgcat tctacggatt gcttaacaaa cactttgagc ttcggggagc gatgttcatc
    28981 cccggctgga ttcccgacct ctccgttccc gagacggtgg cgaccttgcc gttcaatttg
    29041 ccgttcctcg ggaatgaaat ccacctgctg ccgattctct ataccgtgag catgatctac
    29101 tcgatgaaaa tcacccaaag ttcttccggc ggagcggcca gtcaacaggg aatgatgaag
    29161 atgatgacct acggcatgcc gatcatgttc ttcttcgtcc tctacaatgc tccctccggc
    29221 ttgattctct actggtcggt gatgaacgtg atctcggtcg ttcagcagct ttacaccaac
    29281 aagaagaagc aaaaagaagg agagcaggag aaggtcatcc aattccagcc caaggcgaag
    29341 ggaaagaaac gataaaagat caacaccgaa gaggtaccat atatgatacg agaattcgaa
    29401 ggacgaacgg aacaggaagc gattgcgaaa gcgatcgagg aactgaacat cgagcgggaa
    29461 gattttgatg tcgaaatcat cgagcctgct cgcaaagggc tgttcaagaa gaacagcgtg
    29521 aaaataagaa ttcactttga tgacggaatg gatgaagaag agtcctacta tgacgagggc
    29581 gaggacctgg acgagccgtt tgacagcgat ctcgaggaca aggttctcac gttcgtggcg
    29641 acggtactcg aaaagatggg ctatgccggc aacgtttcga tcgccttcag gaaggagcgg
    29701 aagatcggct tgaggatcga cagcgagaac tccaacatca tcatcggccg aaagggcaag
    29761 aaccttgatg cgctccaact gctgacgaac gtcttcgtcg gacagcagaa ctcgaacgtc
    29821 cgagtgattg tcgacagtga aaactatcgg atccgccacg aggagcagct gatccgcctt
    29881 gcgtaccgca ccgccgaaca ggtcgcccga agcggccaaa gccgccttct tgagccaatg
    29941 aatccctatg aacggcgtct ggttcacacc gccctgaacg acttcggcgg cgtggagacc
    30001 aagagcgagg gggacggcct ctacaaacag gtgcgaatca tcgctacgaa ctgacttgac
    30061 gcttgacaag cgtaccgggt gaatctacca tgagtggtgt atcgcatgat acactacact
    30121 atatgttgag tgtataggaa aagggaagtt gggtgaaacg cccacgcggt cccgccgctg
    30181 tgatggggag cgattggaga tgaccactgg agcgatccgg gaaggttcca agagctgcga
    30241 tcctaagcca gaagaccttg cctatatggt cacgccgcca cggagcatgg caggagcgtg
    30301 agagccgaca ggctcttgtc ttcttgtcgc ttcgggcaag gagcaatcat ggttaccatt
    30361 atcaagcgtg atggacggga ggttttctgc gaggcacgga agatcaccga cgcaatctcg
    30421 aaagcggcgc acgccgccgg cgagccgatc gattcggtcg ctcttaccaa tcgggtgctg
    30481 gagcgcatct cgcatcgaac cgccgttgaa gtcgaggaag ttcaagacct cgtcgaagag
    30541 gcgctcatcg aagcgaacta ccccaagaca gcaaaagcgt acatcctcta ccggaaggag
    30601 cggcagcgga ttcgggaagg gaacgccctg atcaaggcga ctcggaacct cttcgattcc
    30661 taccttgaag acaaaacctg gcagatcaaa gaaaacgcca acgcccgcaa gagcgtcaac
    30721 gggatgaaca actatatccg cgagcggctg acggaaagct attggctaaa cgacatctac
    30781 ccaaagacca tcagggaagc tcatctggaa ggaactctcc acctgcatga ccttgggttc
    30841 ttcggaccct actgctgcgg atgggacctt cgcctgcttc tggagaaggg cttcggaggg
    30901 gtggagggga aggtctcttc gaagccggcg aagcacctcc gttccttttt aggccagatc
    30961 gtcaacgcga cgttcaccct ccaaggggag tgcgccggag ctcaggcgtg gtcctccttc
    31021 gatacctact gcgctccctt cgtccgctcc gacaacctgg agtacaagga ggtgaaacag
    31081 gcgattcagg agttcgtctt caacctcaat gttccgaccc gggtgggctt ccaatgtccc
    31141 ttttccaacc tcaccttcga catcaccccg ccgaaaaccc tcagcgagca gccggtgatc
    31201 atcggagggg cgctccaaga cacctgctac ggcgagtatc aggaggagat ggatctgatc
    31261 aacaaggcgt tttgcgaagt gatggaggag ggagacgcct cttcccgggt gttcaccttt
    31321 ccaattccca cctacaacgt aaccaaggag tttccgtggg aaagcgaggt cgtcgacgcg
    31381 atcttctcga tgaccgccaa atacgggatt ccctactttt ccaactacgt aagcagcgac
    31441 ctcagccccg aggacgctct ttcgatgtgc tgccggcttc gcctcgacac cagcgagctg
    31501 cgcaagcggg gaggcgggct cttcggttcg aaccccttaa ccggttcgat cggggtggtg
    31561 acgatcaacc tcagtcggct cgcctgggaa agcgccgatg aaaatgagtt cagggaaaag
    31621 ctgttgtctg ccgccctgct ggccaaagag agccttgaaa tcaagcgcaa gatcatcgaa
    31681 ggagagacgg atcgggggat gtatcccttc agcaaagtgt acctttcgag cgtgaaggag
    31741 agccagggga cctactgggc gaaccacttc tccacgatcg gggtgctcgg aatggaggag
    31801 gcgtgcatca accttctggg aagcagcctg ctcgaggatg cagggcaggc gttcggactg
    31861 agaacccttg cctatcttcg ggaagtcatt caaagcttcc aggagacgag cggcggagtc
    31921 ctgtacaatc tggaagccac ccccgccgaa ggggcgagct accgtctggc gaacctcgat
    31981 aagaaagcgt atcccgagat cgtcacagcg ggggagaagg aacattatta caccaactcc
    32041 agccagctgc ccgtctcttc caccaaagac atctttgaag tgctggagaa acaggatgag
    32101 ctgcagtgtc agtacaccgg aggaacggtc ctccacctct acctcgacca gcggatcaag
    32161 gaggccgcct tggcgaagct tctggtcaaa cgggtgttct cccgttacag gctgccctat
    32221 ctttccctca cgccgacctt ctccacctgc accgatcacg ggtatctcgt cggcgaacgg
    32281 aagacgtgcc cccactgcgg gaaggcgacc gaggtgtgga cccgggtggt cgggtatctc
    32341 aggccggttg aggacttcca tccgggaaaa caggaggagc actcccagcg ctccttcttc
    32401 aaggcggagg cggtatgatc cgggggatcg agggcgtctc cttcctcgat taccccggct
    32461 tgctcagttg cgtcctcttt ctcggaggat gcaactatga ttgcttctac tgccacaatc
    32521 gctctcttct gcaaccggga gaggaagttc tctcactcgg ggaggtcgaa tcctttctcg
    32581 ccgagcgaaa aggcctgctc gatgccttgg tggtcagcgg aggagaggcg acgctgcaca
    32641 aggaaatcac atcattgttt tcgatcggaa aagggttggg gtatctgacg aagctggata
    32701 ccaacggctc aaggcccgag attctcaaaa ccctcttgga agcggatctc ctcgattacg
    32761 tggctctgga tatcaaagcc cctccggagc gttatcggga gatctcaggc ggctccttcc
    32821 tgccggtgtc taaatcgctg aacctgctga agcgacacag ggaacatgag aagacattct
    32881 ccttcgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat cgctgaacct
    33001 gctgaagcga cacagggaac atgagaagac attctccttc gagatccgga ccacgatggc
    33061 cccgacgttg gggagggagg atattgaaca actggcctcc attgtgggag aggtcccccg
    33121 atgggttctc aacgagtacc gggtcccgag aaccttcaaa gaggaggatc gggaccgggt
    33181 gcatcaaaag tatctttgta aagatgaatt acaaggactc ttgccccttc atcccgcttt
    33241 gtgcctaggg tagcgacaag attcgttccc gctcccaggg tagatcaggt tttccgaagt
    33301 gtccgtagtt ggtcgtccgc ctgtagatcg gagcccgtaa attgagcgct tcgatcatcc
    33361 ccttgggggt gaggtcgaag ttccttcgga tgatccgaat caactcatct tcgctcatca
    33421 ctcccgtgca gaacgtctcg acggcgatcg agatcggttc ggcgaccccg atcgcatagg
    33481 agagctgaac ctcgcacacc gtgcagaggc cgctgcgaac cagattggtc gcgatccacc
    33541 gggccaggta cgctccgctg cggtccactt tggagggatc cttgccgctg aaggcgcccc
    33601 cgccgtgatg tccgatcccg ccgtaggtgt cgacgacgat cttgcgtccg gtgaggccgg
    33661 tatcgccggc cggccctccg atgacgaagc ggccggtggg gttgatgtac aatgcggtgt
    33721 tcctgtcgaa gaagccgctg ggttccagag tggggatgat gacctccttt ttgaggaaat
    33781 ccacgagatc cttgtgggcg atctcctgtc cgtgctggtg gctgaggacc accgcgtcga
    33841 tatggtgggg tctgccgtgt cggtaggcga gggagatctg gccctttgcg tcggggcgaa
    33901 ggaagtccgt cttccggcgg acagaagcgg cccgtttcaa cagttcgtgc acccagtaga
    33961 ccgaagcggg catcaggacg ggggtttctt tggtcgcata gccgaacacc atcccctggt
    34021 cgccggcccc gtcgtgatcc acccctaggg cgatgtccgc cgattgccgg ccgatcagat
    34081 tggtgacgga aaggctttcc gcgtcaaagc cgattccctc gatcgtgtag ccgatccgcc
    34141 gtaccgtatc gcggacgatt gcatcaacat caatgtccgc attcgtggtg atctcccccc
    34201 cgacgatcac cgtattcgtg gtggcgaaaa cctcgcaggc gacccggctg ttcggatcgc
    34261 cttccagaca tgcatccagg atcgcatcgg cgatcaggtc gcagaccttg tcggggtgtc
    34321 cttcaccgac cgactccgaa gtaagaacac ggtttcttaa aaacataaaa cccttcctgc
    34381 atgcggaagg gggaactcaa agcaaaattt gctgcgctcc ccttttagca tcgttgttta
    34441 ttgacgccag caagccggga ggccatcaaa tcgacgtcat ggacagatag tagcacgctt
    34501 gagctctttg ggcaagaacc ctagcggtac cgccatcctc cgctttcaag aaggattctc
    34561 agctccttcg ccttctcctc gctgaactgg gctccccgtt gggtgacaat ctcgccggca
    34621 agaatcgagg cgatcattcc cgaggcttcg atgtccatcc ccttggtccg ggcatagagg
    34681 aaccccgccg catagacgtc gcccgctccg gtggtgtcga ccgggttggg ggttccctta
    34741 ggtggaatct ttatgatcgt tccctcgtga gcgatgaacg aacccttgtt tccgtctttt
    34801 acgatcgcga ttctgcagag tttgcccatc gagcggcagc attcgtaggg gtcgtagttg
    34861 tcaaagagga ttcgggcttc ttcccggttc gcaaagacga tgtcgcacga ttctttgatc
    34921 aagtgaagga atgctttgtg gtaacgcccg acggcgaagg gatccgccac gtcgaaggag
    34981 acgatggtgt ggtgctcttt ggcgatcata agagcccgtt cgatcgccct tcgctgggat
    35041 gccgtatccc acatatagcc ggtgaagtgg aagatctcgg cgttttttac cgactcttcg
    35101 gagacgtcgg ccgcttcata caggcgattc gcccccagaa aggtgttcat gctccgttca
    35161 ctgtcttcgc tgatgagaat gatcgtggaa ccggtcttct gcttgtcggt gcaaacgagt
    35221 tcgtcccgaa cccccaagga ggcaaggcgc tcccgataga tcgtcgcata ggggtcgttg
    35281 cccagcttcc ccgccaacgt tgtttccact cccagagatg cgagggcgat gatcgtgttc
    35341 ggacacgatc cgccgcagga gtaggagaca ttccgatcct cgaccatctg cagcagctct
    35401 tcccggcgct cttcgtcgat cagagccatc gtacccttat gaatcccgag ccgggcgata
    35461 tcttcgtcgc tcgcgttgat gatgatatcg atgaggggat tgccaattcc gtagaccatt
    35521 accgtctcct ctgtccgcgc tcattctaac accataaagc agcctcgtac aacgcttgat
    35581 tggagggcgg gacggaagta cgatagagat atgagcgaat atcggatcat cggcggaaac
    35641 gcggcaagcg gcgaggtgct gatcagtggc aacaagaacg ccgcccttcc ttgccttgcg
    35701 gcgactttgc tcaccgacga tccgatccgt ctctccaacg ttcccgaaat tgaagatgta
    35761 aaggtgatga tcgccctgct ggaaagcctg ggttcttccg ttgtccgcga aggcaaaggg
    35821 gtgtatacga tccaatccgg aagcggcggg gcgagcctgc caaaagatct ggtccaagcg
    35881 gtccgcggtt cgatcctcct gctcgggccg ctactcgctc ggaacggcga agtgacgatt
    35941 cctccccccg gaggggatgt gatcggctta agaagattgg acacccactt tatcgggttg
    36001 tccgcattgg gagcaagcgc caagctcaat gaagacggct cgcttcattt caagattacc
    36061 gggagaggcc tgatcgggaa tgatatcttc cttgatgaag cttcggtgac cgcaaccgag
    36121 aacgtgatca tggccgcttc cctcgccgaa ggagaaacga cggtcttcaa tgcggcgtgc
    36181 gagccgcatg ttcaggatct ttgtcatctt ttagtgcgga tgggctcctc gatcgagggg
    36241 atcggttcaa accgcctgat catcaccgga tcgaagaagc tcactggagc ttcctatacg
    36301 ctcggcttcg actacatgga agcgggctcg ttcatcggcc tggccgccgc caccgggggc
    36361 gaagtgctga tcaaggggat cgatcatcgg catttgagga ttctccatct gggttttgag
    36421 cggatcggca tcgcttggga acatgacgga ccctcttcga ttctcgtccc caaaaagcaa
    36481 cgaaaagttc ttgtcaaaga ggtgggaggg cagaccacca agatcgacag cgctccgtgg
    36541 ccgggatttc cggcggatct gctgagcatc ctcaccgttg cagcgaccca atcccgcggt
    36601 tcgattctca tccacgagaa aatgttcgag tcgcggatgt acttcatcga ctggctgatc
    36661 cggatgggtg cggacatcat cctctgcgat cctcaccggg cggtggttca ggggccgagc
    36721 gttctgatcg gatcggtctt gagcagtccc gatgtccggg cggggatggc cctcgtcatc
    36781 gcagcggccg ccgcccgggg ggagtcggtc attcaaaata tctaccagat cgagcgggga
    36841 tatgagaatc tgagtgaaaa gctgacctcg ttgggaatcg acatggttcg aagcggttag
    36901 acgttttttt tgtgcacaag ttatccacaa ggatactaaa caccctataa cccccaagaa
    36961 acagataaga aaaacaaaag gggcgccgtc taaaaataca gagataagct atctgttaag
    37021 tttttatatt aaataaaaat aaactcttgt ttagtggttt gttttactct aaaaccgata
    37081 taagttttat gtggttttta gtaggttgga cacaacttaa cggaactccc tctacccagc
    37141 gtatccacaa gttgtccaca ggctgtacag aagagaaaaa catcatgtaa ttaccttgat
    37201 agcgagagtt agaggtgact ttcccgctgg atcccgccgt cttgttcatc tgataacatg
    37261 tggatagctt gtccctactg ctcttcttcg gcaaactcct gttcatcgat ctcgatccag
    37321 cgattgtttt ttgcatgaag cgatgcccgc ctcaggactg aaaaaagctg gcggacattt
    37381 cccggccagt ggtagctttg aagcagcttg atcgatgtgt cggttaccgg gcgtcgttcg
    37441 gagagggtcg aataaaaatg ccggatgagc agggggatgt cttccttgtg ctcccttagc
    37501 ggcggcatat ggatctgggc atcagtgatc cggtagaaga aatctttgcg catctgtcct
    37561 tcggcgacca gccttaagag cttttcattg ctggcggtga cgagtctgaa atcgcttgag
    37621 cggagcttcg tatcgccgag gcgccgatat tccccgttct ccagcaaacg aagcatatgg
    37681 gcttggaaga tgagcggcag attctccacc tcatccagaa agagggtgga gttgtttgcc
    37741 tggcatagga ggccgatgag ttcgttggtc gcgtcggtga acgctccttt ggcatgtccg
    37801 aagaacaccg atttgccgac agcgtcattg agcagcgagc agttgatggc gatgatgtgc
    37861 cgctggggaa aacgacggcg gtgaagatac tcggcggcga gctcttttcc cgtcccggtt
    37921 tcgccgtaga tgtgcaccgg gcaatctttc gtcgcataga actcaagcag gcttctcagc
    37981 tcgaccatca gcttgctttc accgacaaga ccgctggacc agacgggaga tgattctttg
    38041 acgagttccg tctttccgat tcggcagatc tcttggaaca cctcctccca gttgacggtt
    38101 cgtttgtgca tcaaccggat cttcagatca taggggttgc ggaagttttg aaccggctca
    38161 tcccgggtga agatgataat cggggcggag catcggcttt caccgaggtg gcggagaatt
    38221 tggccggcgg taatcttcgg ctctttggtg tcgatgatga tcatgtcggg agtctcggat
    38281 tcaagggctt caaagagcgc agccggcagg ttgaagagct gaagatatgg ttgggtaaac
    38341 tgtagcttaa accgctcctt ggttctccag tccaaggaga gtacgaatag agacatacgt
    38401 atactcctat cttgttttaa gggtgtaaag gagaggcaaa ggagtagaac gcaaccctac
    38461 catagtctta ccaaagacat tcaacggcta tctactatca ttttgagagg tttttagcgg
    38521 tgaagaagct cttggaggct gccgagcgcg tagatcgccg cggtttcgct gcgaagaatg
    38581 ttgcttttca ggaagacagg gagaaacccc gccttctcca aaaagacgca ctccttctcg
    38641 ctgaaccctc cctcgggacc gatcaggaca ccgatcgggg cgttcggatc gaacgttttg
    38701 gcgtatgtcg agaggtcttt gctttgggag cgggaggact ggtgaaggaa gaggaggggg
    38761 cctctgccgg cccaccactc ggcaagctcc cgaagatcga gcacccggtc aagcaatgtg
    38821 gtgggaactc tcgacccgct ctgctgaagc gcttcggtga tttgagcctc gagccgggag
    38881 aaccgggccc gggtattctt cttatccagc cggacggtgc aatattcgct ggagacgagg
    38941 acgatctcgc cgacgccgag ctcggtggcc tggcgaacga cctgctcctc cttcctgcct
    39001 ttgagcaggc agagcaggag aaccagcgga gtaaagggct cctcgtagga gggcagggcg
    39061 tcggtggagg ccgatgtgtc tccggcgacg gtgagcctga tcgtgcagga ggcggatgcg
    39121 gaagcggtga tgacgccttg gtaacgcacc ccgttgcaat cttgggcgag aaggcgtgag
    39181 ccggccttga ggcgcagcac attcgttaga taatgcgctt ccttgccctt cagggctatc
    39241 tcctcctctc cgtcataggt agaggggagg cggtagactc tcatcgccgg gctcgaatca
    39301 cgtcaatcgc cgcctgcaat tgaggatcga aggcgggatc ggccagcttg cgttcgtcat
    39361 agggaatctt cgcctgatac tcgttgcgga gaagaatcgc taggacttca tcacggattc
    39421 cccgctcccg gttggcgttc acgaaggcag cgatattcgc atcactcggt tcgggatggg
    39481 cggcgatgaa ggatgagacg actttttctt ccatgagctc ggcaaagggc tcgagctcct
    39541 cctccgacat caccaggctt tccacgaaca catccggctc gattcctatc ttgtggatgt
    39601 tttccccgtt gggggtgtag taatgagcgg tggtaatctg agcgaatccc tcgccgaatc
    39661 tgaagacatc ctgaacgatt ccttttccga acgtcttttc gcctaccagc gttgcccgat
    39721 tgttgtcccg catcgccgct gcgaagattt ccgccgatga ggcggaaccg ccgttggtga
    39781 ggacgacgat cggaatctcc tcgctgacct ccgctttgtt cgaggagagg tagcgttggt
    39841 cgcggcttga accgcgcttg ccttgaatcg tcacgatcgt cttgcccgca tcaaggaaga
    39901 tgttcgccgc ctgcatcgcc ccgtcaaccg cccctccgga gttgttccgt tcatcgatga
    39961 tgatcccttt gatttcattg agatcatatt tgcggacgtg ctcaagcagc tgttttccgg
    40021 tttgaggact gaattcggag atttggatat agagaaggtc ttgctccaga agcatcgagt
    40081 caacggtggg agccgtgatg agctcccggc ggagcgtcaa atcgaacgag gttcccatcc
    40141 gatagacggt gatcgttacg ctcgtattgg gctcgcctcg gagcttgcgc gatgattcaa
    40201 ggctggtgag ggcatcgacc ggctcgccgt cgatgtggct gatcagatcg ccggcccgca
    40261 agcccacccg ctgggcgggg gagccgggga agggggagac gatcgtgatc atgtatgtct
    40321 cgggatcgtc cggatcgatc gagtcggggt tgggcttgga gaggtacgtc ccgattcctc
    40381 cgtatattcc gctggttccc tcttcatact ccttcgcatc ggagggtttg acgtagaagg
    40441 agtagggatc gtcaagggaa gcgacgagcg ccgtggcgag atcggtgaag accttgtcct
    40501 tatcgacctc ttcgatatag atcgcatcaa tgtaccgata caggcgctca agagcggaga
    40561 tgtcagtgga aatcttatcc tgagcgaatc gacccgaggt cagaccggta atgagcggct
    40621 cgggatcccc ccctgcaaaa accggggcaa gggtgagcgc aataagaata aacacaaata
    40681 tgtggaaagc tctgaatttt ttcatacagc aagtatactg caacgagtcg tcatcgacta
    40741 ggtttcccgg tattgaccgg ggccatttag cttactacac ttgccgcatg gcaatgtaca
    40801 tctccttccc aagctggatt aaaccggaaa ttttctcatt cctgccgatt cgatggtatg
    40861 ggctgatgta catcctcgcc tttgcgacgg catatctttt catcgtgatc caagcgaaga
    40921 acggcgagat cgccctgact agggaggatg ccctcgatct ggtgatgtgg tgcgtcgtcg
    40981 gcctgattct gggagcccgg ctgttctccg tcctgtttta tgacggcacg acattttatc
    41041 tgacccatcc acacctcatc ttctggccgt tccgcaacgg gaagttcgtc ggtctgccgg
    41101 gaatgagcta tcatggcggg cttttcgggg ccgccgtggg aggatggctg tactccaaaa
    41161 aaaagcggat accgtttttg gagatcgcag atacggtcgt ctacagcgtt ccgctgggct
    41221 acaccttcgg aaggctgggg aacttcatca acggcgaact gttcgggcgg gtctcgacga
    41281 aaccgtgggc gatggttttt cccgacgccc catccttctc cacaaactac gagtgggtgc
    41341 gaaggatcgc cgatgagctg aagatcccct atgagctcgg tcagtacatc aaccttcccc
    41401 gacatcccag ccagctgtat gaggcgctct tcgagggagt cctcctcttt ttcttcctct
    41461 ggttcgtcat caggccgaag cgaagggaga agcccgccgg aacgggattg gcgtggtatc
    41521 tgatcggcta cggaacggtg cgcttcgtca tcgaatattt ccgctcgccg gatgaacacc
    41581 tcgggtacat catcgcctgg gggcgcggtt ccgatacgat cgcccttttt caatccgtct
    41641 tcaacatatc gatggggcag atcttctgtc tgatcatgat cgtcctcgga gtggcgatcc
    41701 gcctcatcac ccactatagg agcaccaaac agtgaatatc atcaatgcac gggtcgaaag
    41761 ccttcggagg gcgatggccg atgccggact tgatggctgg atcatcaacg gaaccgatcc
    41821 ccatcaaagc gaatacgtcc ccgaacgata tcggagcagg gcgttcatca gcgggttcac
    41881 cggcagtgcc ggattggccc tgatcaccaa ggaaaaagcg cttctttggg tggattcgag
    41941 atactttctt caagcggcgg atcagattgc cggcatggtc ttcgagatga tgaagatcga
    42001 caccccctcc catcccgatc cgtacaccta tctgaagatg aacctcccca agggatcggt
    42061 catcgggatc gacgaggcga cgatccccgt cagcgaacgg aggcggatcg aaggagtttt
    42121 tcagaacacc ctcgtcctca aaccgatgaa ggatatcctt gatgccgtct ggaccgatcg
    42181 cccggcgctg ccggagcatg aggtcgtcga ggttcccgtc aactatgccg gctcaagcat
    42241 gcaggagaag cttaggcagg ttcgctctct gatgatcgag gcgggagcga cccacctgct
    42301 gctctccaca ctggatgaca tcgcatggct gacgaacctt cgcgggagcg acattcccta
    42361 caatccgctc ttcatgagct atatgctgat cagcaccgaa cgggcgtgcc tgtataccgg
    42421 cgaaaaacgc tttacggaac agctcagggc acgattggaa gaagatcttg cgattcggcc
    42481 gtatacccaa cttcaggacg atcttcccaa cctgtggaag cacgccgtgc tctactacaa
    42541 cccccaaaag accagcgtga tggtcgaaag gagcttcggt ccctacgaga agatcgaagg
    42601 acgggagttc accaccgacc tgaaagctca aaagaatgag gtggaactgg aaggaatgag
    42661 aagagcccac cttctggacg gcgtggcact ggtcaacttc ctcggtcggc tggacaggga
    42721 aaacggctcc tatacggagt tggagctggc aaagcggctg ctcgatgaac gatccagaaa
    42781 cgagcacttc ctgggggaat ccttcaacgc gatcagcggc ttcggagccc acggggcgat
    42841 cgttcactat gcggcggatg aacaatcgaa cgccaaggtg gaaggagacg gcctgctggt
    42901 cctcgacacc ggagggacgt atgcgttcgg cacgacggat gttacccgga cgattctgtt
    42961 cggaaagccg accgaggagc agatccgcga ttacaccctt gtgctcaagg gtcatctcgc
    43021 ccttgcttcc gcccgcttcc ccgagggcac caacgggtat cagttggatg tgcttgcccg
    43081 tcagcatctc tggcgggaag ggctttccta cctccacgga accggtcacg gtgtcggctt
    43141 caggctcaac gtccatgaag gaccgcagtc gatcagcccc aaaccgatcg tcgcaccgct
    43201 caaggaggga atggtcgtca gcgacgagcc cggaatctat cgaagcggat cgcatggaat
    43261 ccggattgaa aacctgctgg cggttcaaga ggatgaaaag acggagtttg gaaccttcta
    43321 cagcttcgag gtcctcaccc tctgcccatt tgagcgggac ctgatcgacc cggagatgct
    43381 tactgaagaa gagatcgaga tggttgatgc gtaccacgca tgggtatatg acgaactgta
    43441 tgaattgata gacgacgagg cgatcgaata tcttgaatat gcgacacaga gcttcttagg
    43501 agaagaataa gatggtaaaa ccgttacaaa aactgggaaa aatcaagcgc aaaggacccg
    43561 ttgtcctgtt gatcatggac ggggtgggct ttggcaaata caaggaaggg gatgcggtcg
    43621 ccgccgccgt gacgcagacc ctcgaccgcc tctaccggga gaatccctgg acgaagctga
    43681 aggctcatgg aacggcggtg gggctcccca gcgacgatga catgggcaac agcgaggtcg
    43741 gtcacaatgc gatggggtgc ggaagagtct tcagccaggg ggcgaagctg gtcgccaact
    43801 cgatcgaaac caaagcgatg tatgaagggg atacgtggaa gaagctcatc cggaatgtca
    43861 aggagcacga ttcgacgctc cacttcatcg gactcttaag cgacggaaac gtccactccc
    43921 acatcgacaa cctcaaggcg atggtcgccc aggccaaggt cgaaggagta tcgagagttc
    43981 gggtccacgc ccttctggac ggacgggatg tcggggagct ctcggcattg gagtacttcc
    44041 agccctttga agcgtttctg gaagagttga acgacgaaga cttcgatgca agaattgcaa
    44101 gcggcggcgg ccgaatggtg atcacgatgg atcgctacaa cgccaactgg aagatggtcg
    44161 agaaggggtg gcatacccat gtcctcggcg aggggcggct cttcgcctcc ccgacggaag
    44221 cgatcgagac gctgaggaag gagaccggag cgatcgatca ggaccttccg cccttcgtca
    44281 tcgccgaagg gggcaagccg gtcggaacga tcgaggacca tgacagcgtg atcctcttca
    44341 acttccgcgg cgaccgggct cttgagatca ccaaggcgtt cgaggcggag gagcttatag
    44401 agtttgaccg aaagcggagg ccgaacgtcg agtatgcggg aatgatggag tatgacgggg
    44461 acctgcacgt cccggcccaa tacctcgtct ctcccccggc gatcgatcgg acgttggccg
    44521 agtacctcac gaagagcgga gtgcggcagt tctcgatctc cgaaacgcag aagttcggcc
    44581 acgtaaccta cttcttcaat ggaaaccgca gcggcaagtt cagcgaaacc ctggaggagt
    44641 acgtggagat cccctccgac acggttccct ttgaagatcg cccatggatg aagtgcgccg
    44701 agatcgccga ccgggtgatc gaggagatcg aaagcggcaa gtgggacttg atcaagctca
    44761 actttcccaa tggcgacatg gtcggacata cgggagtcta tcaggcggtt gtctgttcga
    44821 tggaggggat ggatctgcag atcggacgga tcgaaaaagc ggttcgagat gccggaggag
    44881 tgttgatgat caccgccgat cacggaaacg ccgatgatat gtatgagcat accaagaccg
    44941 gcgaagtcgc ccgcaaagag gacggcacac cgaaagcgaa gacgagccac tccctcaacc
    45001 cggttccctt cattctggtc gatccgctct acaagggtga atatgaaccg acattgaagg
    45061 aaggattggg catcagcagc gtcgccgcca cggtgatgaa tctccttgac ttcgatccac
    45121 ccgaattcta cgacagctcg atcatcacga tgaagtaaaa gatcttttga tacatcctcc
    45181 gattgaaacc tgctaagatg gaagcaggag gatcggatgg gtattgacga tcgattgaag
    45241 cgcctgagcc gccttcaagg aggagatccg gggatcttcg ccctggcggt ccattccttc
    45301 atcgaagggg agctgcgccg gcggtacgac cttccctatg aagaggagcc gagcttcaat
    45361 gcgctcagcg accgactggt cgaagaagtg ggcgaaaaaa gccggcaatt cctggcgaat
    45421 ctcaaagccg cccacaaggc ggtgaatgag gtccgtcatg agttcaatgc tctcggcgtc
    45481 gaccacgcgt tgatggcggc ggctcatctt tacacgttct gttactttat cgatatcgat
    45541 gaaggggctc tgcagacgct ctataagatc aaacgggaat tggatgatac ctggacccgc
    45601 tcggagagcc ggggcgaact gataacaagc aatcaaatcc tccaaacgga gctccaaaaa
    45661 atacttgccg agaagggatc gctctccgat cagctttccg aactgaagga acgggaggag
    45721 gaacgagagc aggttgatga tcggatccat tcattggaac atcagattcg gaaccttttg
    45781 aaagacaggg atcgaaacga aaaaaagatc aaagaactcc gggatgaacg gcatgcgctg
    45841 caagtacagc gccgctcttt ggaaaagcag acccgcaatc ttgaggagta tctgcacgtg
    45901 atgcggacga tgacctccta cacccggacc cgccgggatt atgaacacct tatcctgcga
    45961 ttatccgctc agcaggagga ggcgcttcgc cgaatcgatc tgagtagcga ctttttgatc
    46021 accggtggcc cgggaacggg gaaaaccctc gttcttctca aggcgatcgg aaagaaatcg
    46081 gaaaagagca acgtcgccct tctcacctat acgaactccc ttgccaaata cgatcaatat
    46141 ctgactcggc tgcttggcat cgaaatgggg cagatcatga cgatccatca ctttcttcgg
    46201 gagcggatag aagaaggaga aagcatcaag gtgctggccg gagaggatac ggacattctc
    46261 atcaggcttt gtgagccgta cgcctctttg cagtggaccg ccaaagatat ctacgatgaa
    46321 atcgttcacg ttctttgggc gttcggccat acccgccagc gctactgcat cgatctggtt
    46381 caacgatccg gaatggagaa agagctcgat gaagcgatgc gaagccggat ctgggaggcg
    46441 aaggaggctg tcgaagcgtt catcgaggag acgaaacagc ttccttttca atatttcccg
    46501 atccaagcgt tgaaggtcgc cgaggagcat cccgttacga tcttcgatca cttgtttatc
    46561 gatgaggcgc aagatgccac cgccatgacg ctcaccgccc tgaagcggtt cgtttccaaa
    46621 tcgatcattc ttgccagcga cggggagcag gcgatctatc agccgggctt ttccttcact
    46681 caagcgggga tcgatgtatc cgacaatcgg gtaatcctcc aagagaatta tcgctccacg
    46741 ttgcagatcc accggcttgc ccaacggttt gagcggcgaa gcgaaagcga gcaatcaaga
    46801 gcgctgcgcg atggtccgaa ccccgaactt caccggggtc gtgatggatc ggtgttgcag
    46861 aagttggtcg tcgagcgggc aatgttgttt atcgaacggc ttgagtacga ggaagaaaac
    46921 atttgtatca tgtgcccctc gagctcctgt tatccggagc ttgagcaggc tttgacgaag
    46981 gcggggattt caagcgtccg cgtcaatgat gagcgcttca cgtttggtga ggagtgccac
    47041 gggcatcttc gcctctccac cctgcattcg gccaaaggtc tcgatattcc cgtcgtgctg
    47101 ctctatctgc cgtttctccc ccgagtgaat gaatcctatg atgaatcggt caacaatgaa
    47161 ttgattcgaa agctcctgta tgtaacgatg acccgggcga tcgatcatct caacgtcttt
    47221 acgcttgtca gtccgaaaga ggagatcatc agggagttga tcgccgcatt ttagatatgg
    47281 tatgaacgcg ataccagcac gaggattgtc gcccccgctc catagataat gaggccgatg
    47341 atgttgtaga aggtccgttg gctgatcgag acgaaggcgt tgcctacggt aaggagcagg
    47401 aaggcgacgg tcttgaagcg ggtatcccgc ttggtgtgtt cgcccagcgc gatcacgaga
    47461 taggataggg cagctacgac ggtgagggtg atgcagatcg acaggaagag cgaatcgctt
    47521 actcctgcct ccaggaggaa ggaggggtcg ttggggctgc tgggaaccag cagggccaga
    47581 agcgcggagc ctgccgcccc cacgaaggtt atgaaaccga tgcgcgaggg gcttaaccgt
    47641 agaaacagca gcgacccgat caaaaacagg aacgaggtat acagcaggct tccatggtag
    47701 atgaaggtca ggactcctcc gtccagaaga aacgttccgg taatcgcctg atgaagggga
    47761 aggattttaa tgttcgcaag aacgatcgtg agaaagagca ccggcaggag ctccgcttcg
    47821 ctgccgaggt tcgcccgata tcgggtagcc agaagcaggg agacaaggaa ggtgatccat
    47881 atatgaatca acgccggcca gaggtgggcg cgagcctcaa ggacaaggtg tccatcctgt
    47941 gaagcgatga gggtgacgat tacgacaaaa cttagggcca tggtaaccag gccctgaatg
    48001 aggttgaacg tgaaccggac ccctttgctc atacgatgtc acgatgagga gtctttacca
    48061 gaacacccat tcgactgcag gtgtacgccg ctccgaagga ggcgacgtat gtatcgatga
    48121 gaatgccgac accgttgaga atcggcatca atgcaaccat cgacatgtga gctccgtaga
    48181 gatcgatctt gaggaagttg agggtgaggg tggtgatgaa gaagacctca aaccccacgt
    48241 acaaaggact gacaaaggag aagagcgccg aagcgagggc gatggtgaga agaatcatgt
    48301 cttcgggcac gcttcccgtc gccgaataga tcatcgtcag cgtcgcgagg gtcgagacga
    48361 gcgcggagcc gcccctgccg atcaaggtat agagcggaat cgccgttccg ctgacccgtt
    48421 tctggacact gaggttttgt ctcgccaggc tcatcgtggt cggggtggtg aagaagatcg
    48481 agccggtgaa catcccggca agggcgggag cgagcatccg gtacagaatc cgataggggt
    48541 tcaccgaaaa gagggagaag atcgcaagaa gaagcggcag aatgacgaag agggcgagaa
    48601 gcaccccaag acccaagatc atcaagaacg gcaacatcac gaagacggtg ccttccttgg
    48661 cgacggagat cagccaatgg gaagagatga agaagagaaa gatcgctcca cattgggccc
    48721 acgctctggc gagccggaag aaggtctcgc tgaaggagtt catgacgacg taggcgggac
    48781 ggatgatctc gacgttcggc tttaaaaacc acccgaggaa gaaggcgatg atgatcaccg
    48841 gcaggagaaa atcatccgct ttcaccagcg tgtagaaggg gttgacgatc gtaaggtgtc
    48901 ccagaaaggt ggagaacgat tgcaatccga cacttgaaaa ggtgccgggg tccgtgcccg
    48961 ccgaagagga ggcggggaac ggcaagggca ggaagcggaa gaccgccgct gcgctcaaga
    49021 caaggagcag ggtggaaaaa acactccaga acacggtggt tcgagcaaac gtgcagcctt
    49081 tgccgtcttt ccgcaaggaa gcgatgccgg cggagaagct gaaaaaaacc aaaggaatga
    49141 ggatgaaccc gccaagttgg accagcacag cggtgacggt atacgttgct tgctggaaga
    49201 aagaggactc accgaagacc aaggttgccg ctagcccgaa cgctgttgcc gctaagtaac
    49261 taatccacgt tttcatacaa attagggtac atcatcaaag gtagcaagtc aatcaaagga
    49321 aaggaagtgt ttgtcggaag gaaccatcaa atcagctgat gaattccatc aaaagaaaca
    49381 cctaaacagg aagggatgtc atttggaaag agggaggggg aacacgatgc ccaattcttt
    49441 tccgatgctc ttcaattgtg aaacgacgtt cgctgacaag ggaacaccct ccacatcaga
    49501 gcgggcctcc gcttcctgct ccttctgccc tgcccacaac acccgcttgt gtccttcggc
    49561 gacgggtgtt tcattgagtt cggataagag cgcatcgaca tcgtttttga gctcatccgg
    49621 atcgcggaac aggtcgaggc ggatcgcacc gaagaaatgg cagacccggg cgctcgtcgc
    49681 ttcgctgtct ttcactgatt ttccgaacgt accgccgctt aggattgccg tcatcatgtc
    49741 gacgaggacg gccaggccat agcccttgtg tcccccgagc tcctcgccgg ctccgccaag
    49801 cggaaggagt ccgccgcccc gttgatagag catgtcatca agaagccggg cggcatcgga
    49861 ggttaccgct ccggtcggat cgaccgccca tcctgaagga aggggatgtc cttcccggtt
    49921 gtacacttcg atctttcccc gagtcactcc cgtcgtggac atgtcaaggc tgaacaactt
    49981 cccctcatgt ccgggaatcg ctacggcgat cggattcgtg ccgaacatcg ccgtggttcc
    50041 gaacgtggga acgccaaggg cggcggtgtt tgtcatagcg atgccgatca tatcgcttcg
    50101 ggcggccatc tctgagtagt atccggcgat tccgaagtgg ttgctgtttc gaatggaggc
    50161 gaatccgaca ccggaaacct tcgccttttc aatcacttga tccatcgtct tcctgctgag
    50221 gctcatgccc atcgccccat tggcatcaag gacgagggag atcggggttt cccgaagaat
    50281 cgtcggttga attccgccgc tcatgatccc gagccgaagg ccgttttcat agcgccagag
    50341 gcgggcgaca ccgtggcttg cgattccgcg ggcatccgca ctgacaagaa tctcggagga
    50401 gtcgaccgcc tcctcctctg aaaggccgag ggcgatgaaa acgtgagtac aaaagtgttc
    50461 caatgccgtt cggtcgatat ggtacggttc catccttgtc actccgggag ggcgaggagg
    50521 cgtttgagtc gataggaggc gtttaattga gcatcgaaaa gggttttcac gtaggaggtg
    50581 ggatcgaact ccggctcgtt cattgctatg gagagcgcac tgaggaagat gagctcctgc
    50641 atcctttcct tgtcaaacca gatgaccccc tgatactcat tcaccaagcc gtaggtgcga
    50701 acccgttcct cctccaaaag tcgggcaagc gtttcctgtg gcttctcccc cttcctgtac
    50761 gctcggtggg cgcacaccac gatcgcaccg ctttggcacg ctttgcgctg atcatcaccg
    50821 acaaatcccg ctttgttaag cttctcggcg tagaagcggc agagctgaag ccgttcggca
    50881 agggtgatca cctcttctat cccggcgtca tctccgacga acggattgag gaagaacgcc
    50941 gagctgacga tcacctccag ctcgtcaagg agcagccggc tttggctgaa gagacgggtg
    51001 gagtggtcgg caaagagggt gttgaggcgt ttgacggtct ccatgatcgc ttttacatcg
    51061 ggatcacgcg gcggatggtt gaggatcttt ttcgtttgag ggtgaagcag ctcgtaggcg
    51121 gtcgtcaggt gagcatacgc ttctcccaag gagaggagaa gcgttcgttc cgctttggag
    51181 gagcttgtcc ggtcaaagat cgacgaaagg agggagaaga agctttgcga gcggaggttc
    51241 tccatcgcct cggtgaccgg gcggaggcgg agagccatga tctcctcttc aaagttggcg
    51301 attccctgac cgccgattcg ttcgtacagc tcggcataga cgccatcgga gtcttcgact
    51361 tcacggatat tgaggaacac cttcgtttca aacccccaga ggtggaccca gagtccgtca
    51421 tcaaagagct tcagtgaagg actcatatag gtaagcttgt cggtgaagga atcccagatg
    51481 acgaagcgac ggccgcttac cgtcaacccg agactttccg ccagcgactt ggtagcggtg
    51541 tgcttgcccc cgttttgttt tatcagcttg ggagaggagt gctttatcgt tccctcgacc
    51601 ttctcatact ggttgttgta aaggatcagg gagcgctcgg tatccgttcc gttgacgtag
    51661 cagaacgccg attcatggac gtgtccgtca tgaaccagat caaagagctc aaagtactcc
    51721 acatcgctgt agaggtagcg gcgcttcagc agagggaaga ttctccggta gtgttcatcg
    51781 acgaggtgtt gattggccgt ctcgtcccag taagcccggc gatactccat cccgtatttt
    51841 tccctgaatc cctcaacctg accgtgaccg aacatcggaa ggcccggcat cgttgcaagg
    51901 agggtgcaca ctccaaaata cttgtcgccg tcgccgaatt gggcgatggc ggtctcctcg
    51961 tcggggttgt tcatgaagtt cacgaagcgc ttgaggattt ccggttcata ggtgagggtg
    52021 tttttgatcg tatcccggta ttttttattc tcctggtttt tgagcatgtt catgaacgcg
    52081 ctgttgtaca cccggtgcat tcccagggtt cggacgaagt agccctccat catccagaac
    52141 gcttcggcaa gcaggagggt gtcaggcaat tcctcattga tccggtcgac gacctcccgc
    52201 caaaactcat tggggatcag gcggtggaac gtctcgtcat cgagtgaatg gggaactcgg
    52261 ccggcgatgt cgccgcctct gccggggcgg ggataccaga gccgttgaat gtgcttcttc
    52321 gccaacgtca tcgccgcatc gaagcggatg atcgggaagt tcatcgcgac gtgcttgatc
    52381 tgctgataca ccatctcccg ggtcttcggg ttgaggaaat cgagctgggc ggtgtcgttc
    52441 cagggcatcg atgttccatc gttgccgtgg aagatatagt gtgtctcacc ggtattgagg
    52501 tctcttcttt ggaacgtgac cgctgcgtcg ctacggtcgt agtagtggtc ttccagcttg
    52561 atctcaacgt tgggattgga ggagagattc ggtccgttga acgtgtagga ggggaagggc
    52621 ggatgtctct gccagatgaa gaggtcgggg ttgctcatca cccagtcgct gtcgatgccc
    52681 gtgtggttgg ggaccatgtc gctggcaagg cggactccat acttggaaca ggtatggcga
    52741 agggaatcca aagcgcccca tcctccgatg agggaagcga tctcgtagtt cctcaaggag
    52801 tatgccgaag cttcggcatc ggggttgcct gtaaggttct tgatcatctt gctggcgctt
    52861 gagcgctccc agatcccgat cagccacaga gcggtaaacc cccgttgggc caacaactcg
    52921 atttcccgct ccgggatctt gtcgagcgta tcgatcggat acccgtactc ctttgtcagc
    52981 tgatcgagcc ataccaacgt ggatttcgcg atcattacga cattgggcat ccagtttcga
    53041 tcggcggtga acgcttcata ctcatgatcg aagagggagt agtcgatcac cggcatcgga
    53101 ggagcgggac caccgggtgg aaaatggggc ttttcctcct cttggacgta gtcgatcgcc
    53161 cgcagcagaa ggcgtttgag ctcgctgtcg atcatgtccg cccagtgctc gaggatgtat
    53221 cgaatctggt cgaggagcga atcgggatag aggcgggcgg gccgggtaag gaacgaaaag
    53281 agatcatcat cttttgccgc ggtgcgctgt tgagcatacc cgcccatcag ggcggtgagg
    53341 gcctggtggg ctttggggaa gaacagtccc tccggcttca acagaggttt ggaggcggcg
    53401 atgagggcgg ggttttcctc catcacctga tggatgaagt agccgcgaag ggattcgagg
    53461 tagtaaaact ccaccggata cgatttcgag acaagcaagg gcgaggggaa ctccttcatg
    53521 tagaatgtca atacttcctc gatatcgtgg tttttcccca ccaaggttcc gagccggctg
    53581 aagaagtccg gttcatgtgt gttgaggtag ttggaaagaa cgctttggta gagctggtga
    53641 agcactgcca ccgcgtgaag ctttccgctg gaaagataat cgttaccgcc cttgcttcga
    53701 accttctcat tgtaccggta tgcaagctcg gtggcttcct gatagagctc gttcaagtga
    53761 gcgtccgagg gaaagagcgg gggaaaaaaa tcaagctcgg tacgggttcg tacggagatt
    53821 ctgaaatcgc gtggaatcaa gaggcacctc ccaaaccaat ggtaatgtga gtatatcacg
    53881 ccggggtgat gttgtaaccc cttcttagag gaggacccgc cgggactccg tggcttctct
    53941 gccggaaaca tcaaatttat gcgtcggttt ttgaagcgtg gcaaccatat tttcaaacaa
    54001 atatggagta tctttaaaat acaaatcgct aactgcagtt agcgagttgg agcagacgga
    54061 cggttctgaa cgaacctatt aattcaagaa tttattgtag cccgaaaatg atttttccta
    54121 ttcatattaa gctctacttc ttagtttttt tgcttttttc gtattagaat ctttgcaaca
    54181 ccacgatcct tctcagagga ggattcgctg tgtgggagtc ctgcagtcaa gaagcttcgc
    54241 cgcctcccgt actatcaggg gaagagagat ctcctcctca ggggtgaacc gggaaaggac
    54301 gaatgaggac accgtcccgt ggatcggtcg gccgattccg attctcagcc gccagaaatc
    54361 gcctgagccc aaggcgcttt ggatcgattt caatccgttg tgtcctttca accctccccc
    54421 ttgctggagg cggatcgttc caaatgcaag ttccagatca tccgagacca cgagaatttc
    54481 atcggccttg attccgaagt aggagcttgc cagtccgacc gaccggccgc tttcattcat
    54541 gcaggtcaac ggtttcaata aatggatcgg tccgtctttg gcgattttgg ctgaaaactt
    54601 ctcgctccag gcgatcgaac cgtacgcctc ctttgcaacc atccacccga cgttgtgccg
    54661 ggtcggttcg tagatttttc cggggttgcc gagaaagacg atcagtttca tcatatcgga
    54721 agtgtaaaag aggggctcct ccatgacaag atggatgctc tttcgtatga tgtgtccatg
    54781 tatgctcttg tattctttgt tccgacgacc cagcttgaag aggtgaagaa cgccctgttt
    54841 gcgaaggggg ccggtagatt gggctcttat gaacagtgca gcttccaagt caggggcgag
    54901 gggcaattca ggcctttggc gggaagcagc ccgtttctcg gaggtgaagg cgtgcttgag
    54961 aaggcggagg agtggcgcgt ggagatgatc gtttcaaaag aagttgtcaa cgaggtcgtc
    55021 gaagcgctgc tttccgccca cccgtatgaa atgcccgcct accatctcta ccccgtattg
    55081 accaaggaga gctttgatgg gcattcgtga agaaatccga caccgggtcc tgctcctcga
    55141 cggggcgatg ggtacgatga tccaaagtct tcggctgcct ccttccgcct tcacctatcg
    55201 gggaagaacc gccgcgggcc ttgccgaagt cttgaatctt acccaaggtg aagcaatcac
    55261 ctcgattcac cgggcgtata tcgaagcggg tgcggacatc atcgagacca atacgttcaa
    55321 cgccaatcgg atcagtttga gcgagtataa ccttgaagac gaagtggcgc cgatcaaccg
    55381 gtgggcggtc gccctcgcca gggaagcgag cagggatgcc gagcgaaggg tgtatatcgc
    55441 cggagtgctc ggtcccacag cttccggcct ttccttttcc gccaaagtgg atgatcccac
    55501 cgcccgaacc catgatttcg acttcttcat tgaggcctac cgcgagcagg ccaaagccct
    55561 catcgaagcg gggtgcgatc tcctgctcat tgaaacggtt ttcgacacgc tggttgccaa
    55621 aagcgcgatc attgcctgcc gggaggagct cgaagcggca ggcaaagacc ttccgatcat
    55681 ggtcagcgtc acgttcagcg acggcagcgg gcgaaccttg agcggtcaga gcctgggggc
    55741 gttcatcgcc accctcgccc ccttcgatct cttcagcatc gggctgaact gctccaccgg
    55801 accgaaggag atgattcctc tcctaaacga acttcacgcg atctccccct gttatacgtc
    55861 ggcccacccg aacgccggct tccccgatga ggacggcagg tacaccctcg ctcccgatca
    55921 ccttgccgga gagctgaagc cgcttcttgc agaggggttt ctgaacatcg tcggagggtg
    55981 ctgcggaacg acccctgacc acatcagggc tctcaagaga atccttcctg caagcaggcc
    56041 tcgatcggtt ccaccgaaaa agcccgctct gctgttggcg ggactggatc cggtcgaggt
    56101 gaagagggat accctgctca tcgtgggcga acggacgaat gtcgccggat cgcggatctt
    56161 ccgccgccgc atcgaacgga aacaatggga agaagccctc ctcgtcgccc gggatcagat
    56221 cgccgaaggt gccgatcttc tggatatctg catggatgat ccgctcttgg attccaaaga
    56281 ggcgattgtc tcgttcttac ggtatatcgc cagcgacccc acgatcgcaa aagcggccct
    56341 gatgatcgat tcctccgact ggtcggtcat cgaatcggcc cttggggagg tccaggggcg
    56401 ggggatcgtc aattcgatct ccctgaagga aggagaggag ctcttcatcc gccgggcgaa
    56461 aaagatcgct tccttcggcc atgcgttcgt ggtgatgctc ttcgacgaag cgggacaggc
    56521 tacaagctat gaacggaagc ttgcgatcgc cgagcgttct tacaccctcc ttgtcgaggc
    56581 gggaatcgat ccctcttcga tcatcttcga tccgaacgtg ctggcgatcg gaaccggcat
    56641 cgccgagcat gacgcttatg ccaaagccac gatcgatgcc acccgggaac tgaagaagcg
    56701 gtatcctcgt tcgtatataa gcgggggaat cagcaacctc tccttcgcct ttcggggaaa
    56761 cgatccgctt cggagcgccc tgcatgaggt gtttctggag cttgcccgct tcgacctggc
    56821 gattctcaat cccgcatccc ggaatcaatc cacccttgat gaacagaccc ggaagatcgt
    56881 ccgaaaggcg ttgctcgccg gcgattcctc ccaacttctg aaccttgccc tggctgagca
    56941 ggcaaaggaa aaaatccaaa acaaccgagc cgatctcaca ccggaagagc tccttgcccg
    57001 ggcgatcatc gaaggagagc tcctcggtct tgaagaggtt cttgactctt tgacccatct
    57061 cgaaccgctc ttcatcatcg attcgatcct gatggaggcg atgaagaccg tcggtgcccg
    57121 gttcggcgag gggaagatgt ttctctccca ggttgtccgc tccgcccggg cgatgaagaa
    57181 cgccgttgac cgcctccagc cgcgtcttga ggctcaatca gcagagaaga agagctcttc
    57241 aggcactcga aaggcggttc tcgccacggt gaagggtgat gtgcacgata tcggaaagaa
    57301 catcgtcgcc ctggtcctga gctgcaacaa cttcgaggtg atcgatcttg gcgtgatggt
    57361 cgatgaagag acgattctca acgcggtgaa ggagcacgac cccgatctcg tcggtctttc
    57421 agggctcatc accccttcgc tcaaggagat ggagaaggtc atctccctct ttgaagcgga
    57481 aggccttcag cttcccgtct tcgtcggcgg ggcgacgacc agcgaggtgc acaccgccct
    57541 gaagctcgct ccgctttcaa ggggcgcggt gatccggacg aaggatgccg gcgagatggc
    57601 cctgaccgcc gtcgaggtgc tcgggccaca aggtcaagct cgcttggaag cgattcgaac
    57661 gcagtacgaa gcgattcgaa tgcgctatac ccaagaagca gagccctcca gaggatcaaa
    57721 agaggatctc cttgaaaaac aggagcagaa acaaacgggc ctgaaggcga aacggtatgg
    57781 agtcttcgtg aaggatgatt ttgatctttc cgaattggcg aagcggatca gctggcggat
    57841 gtacgttcag gggtggggcg ttccctttga ggatgaggag gcgaagcgga tcatcgccga
    57901 ggcgaaggag cttcttgcca gggaagagat cgcatcctcc ttcgaaaggg gaatgaaggt
    57961 cgtgtattcg atgatgaaag tcaaaagcga ccgccatacg atttccaccg gtaggcatgt
    58021 ctttcatttc cttcgggatg aaggaagcgg cttcagcctg cccgacatgg tggcccaaga
    58081 ggacgccgtc ggcttcttcg ccgcctcctc cgctcttgat ctggaggcga tcctcgatga
    58141 tgatccggta aagaccctga ctttgaagct gcttgccgat cggcttgccg aggttcttgc
    58201 tcaggaggct caatccctca tcgccgatct gtggacggaa agaccccgct cgatcattcg
    58261 gcccgcgatc ggctacccct catggagcga ccacagcgag aagaagaccc tgttcgagct
    58321 gttggaggtg aaggagcgga tcggcatctc gttaagctcg agctatgcga tggatcctcc
    58381 atcatcggtc tgcggggcgg tcatcggggg cgaaggggtc cggtatttca ccgtcaagga
    58441 cgtgagccgg gcgcaacttg agcgctacgc ccgaagaaaa ggcgttcccg atcgggagct
    58501 tgcctccttt ctgaaggata tgggatactg agccatgcgt gtaaccgaaa tcatccaagc
    58561 ctccaaaaaa cccctcttca ccttcgaact ggttccgccg ctcaaaggcc ggacgctcga
    58621 gcccctgatg gaaacgatcc ggaccctgat ggagttcgac ccttcctaca tcaacgtgac
    58681 gaaccaccag gcggaggttg tctacattga gcgggaggat gggctccttc agcggaagac
    58741 ggtacgcaag cgccccggca ccaccgccct ggcggcgctc atccagcaca ccttcaacgt
    58801 tccgacggtg gcgcacctga tctgcggcgg gatgagcagg gagcagatcg aagacgtcct
    58861 catcgagctt cactttcttg gaatagagaa cattctcgcc ctcaggggcg accctcccgc
    58921 cggggagaag cgcttcatac cgatgcccga cgggcatgcc tactcaagcc agctggtcgg
    58981 gcagatcgta gatctcaatc acggaaggta tctggatgac cagtacaggg acggggaagc
    59041 gacccggttc tcgatcggag tggccggata ccccgagaag catgccgaag cgctgaacat
    59101 gacggtcgac ctggcgaacc tgaagaagaa ggtcgatgcc ggggcggagt acatcgtcac
    59161 ccagatgttt ttcgacaacg gaaagtattt ttccttcgtg aacgcgtgtc gctcgatcgg
    59221 gatcaccgtc cccatcatcc ccggtctcaa gcccatcgcc tcgaagcggg atctcagcct
    59281 tattccgcaa accttcggcg tcgatcttcc cgaagcgttg acttccgagc ttgaaggggt
    59341 cgattccaac gccgagatca ccgaaatcgg cgttcaatgg tgcatcgccc agtcgaaaga
    59401 gctctatgcc gcaggggttc cctccattca ctattatacg ctggggaaag cccggaacgt
    59461 ggccaggatc gttcgcgccg tctattaacg ggttttggtc actccgactc tgggacacac
    59521 cgtttcttcc ggacagcggg agcagaaggg gctgaccggg gtgcagatca cctgtccgta
    59581 gcggacgagc agttcgttca aggggatcca gaagcgccgg ggcatcacct tcatcagcgc
    59641 ctgttcactc tcttcgggag tcccggtggc gatccatccg aggcggttgg cgatccggtg
    59701 gacgtggcag tcgacgcaga tcgcatcgat cccgaagccc aggttcaggg tgaggttcgc
    59761 cgtcttgact cccacccccg gcagccgcat cagctcctcc tgcgtgtcgg gaacgacgcc
    59821 tccgaactct tccagaatca tcctgctgat cgagcggatc gtcttcgctt tcgtcttgta
    59881 gaagccggaa ggatagatca gctcctggat ctcttcttcg gtgagatcaa ggatcccgct
    59941 cggagtcttc gcgcgggaga agagggatcg actcgctttc agggtcaccg catccttggt
    60001 ccgcagggag atcaccgtgg cgatgaggat ccgaaacggg tcgtcgtgct cttgtgcgac
    60061 cagagaaacg gagggaagaa tcgttccctg tttttcaagg gcaaatcgaa agcggtggaa
    60121 gagggaatcc cagtagtgtt cgtccatagg tatcagctac cacaggtcgg agcaaatgac
    60181 aaggtgccct acccaccggg gatgcaagct ggtacactgc aggaaaggag tttgtgatgt
    60241 ccaagataga aatttgtctg gattccgtcg aatcgatcat cgcttccgaa cagggcggag
    60301 cggaccgggt ggagttctgt tccgacctct tcgaaggggg cctcactccc tccttgggaa
    60361 tgttcaaggt cgccaagggg cggacagcga ttccgatcaa cgtgatgatc cgcccccgcg
    60421 gcggggattt ctgctactcc gatttggagt tcgaagcaat gaaggaggat gcccggctct
    60481 ttaaggaagc gggggcgaat gcgctcgtct tcggtatcct cacccccgaa gggaagatcg
    60541 acaagacacg aagcagggaa ttgatcgcca tcgcccggcc gcttccggta accttccacc
    60601 gggcgttcga catgacttcc gacccttttg aagcgatcga ggatctgatc gaactggggg
    60661 tggagcgggt cctcaccagt ggcggggaag catcggtcct tgaaggagcg gacctgcttg
    60721 cccgattggt caaagagtat ggaaaccgga tcgtcatcat gccgggggcg gggatcacga
    60781 agcggaactt cgaaaagatc cattcaatca ttaaggccga tgaatatcat ctgtatcttc
    60841 acagcgaaca gccgtcaaag atggtccatc ggaaagatca tgtctacatg ggtggattgc
    60901 tccgccagga ggagtacctt gttcaacaca ccgaccaaag ccgggtagaa gcgatgatcg
    60961 ctctggtgcg ctagcgtcat ctcttgattt tttccccctt tccctctagc gtttcttcat
    61021 gatactcatt ctcaagaaac agataaccga agctcagaaa catgccattc gcgagtttct
    61081 ctccgacaag ggatatacgg tcaaggagat catcggtcaa gaggaaacgg tcttcggggc
    61141 ggtcggccaa tcctccgtcg acatccgcca agtccagctg cttgaaggtg tcgcctcggt
    61201 ggtgccgatc agcaagccat ataagcttgc ttcccgggag ttcaaaaaag aggacacgat
    61261 cgtctcggtc ggcaaggtga aggtcggcgg caaccgcatc gcgatcatcg ccggaccgtg
    61321 cgcggtcgaa tcacgagagc agatcatggc ggtcgcccga agcgtccacg acgccggggc
    61381 ggtgatcctc cgcggcgggg cgttcaaacc ccggaccagt ccctatgcgt ttcagggcct
    61441 gggggaagag ggcctgaagt acctccggga agcgggagat gcctacggca tgccgatcac
    61501 cagcgagatc gtcagtccct ccgatgtcaa tatgatggcc aagtatgtcg acatgttcca
    61561 gatcggagcc cgcaacatgc agaacttcga actgctcaag gcggtgggga agacgaagat
    61621 gccggtcctc ctcaagcggg gcctctccgc cacgatcgag gaatggttga tggccgccga
    61681 atatctgatg gccagcggaa ccgatcaggt catcctctgt gaacggggga tccggaccta
    61741 cgagcgggcg acaagaaaca cgttggacat ctccgcggta ccggtgattc agaagctgac
    61801 ccacctcccg atcatcggcg atcccagcca tgcgaccgga atgcgggaca tggtcggttc
    61861 gatgagcctc ggccttgtag ccagcggagc ttccggcctg atggtggagg tccataccga
    61921 cccggacaag gcgttcagcg acggtccgca atcgttgtat ccttcccagt tcgaaaagct
    61981 gatgcgggat ctcgacgccc tcagcggtgt gttgggcaaa tcgatggagc ggccggtcaa
    62041 agttgaaggg ccgaagaccg gggagactga aaagaagaag atctcttcgc cggtggtggc
    62101 gttccaaggc gagcggggag cgtacagcga gatggcgatc aggcgggcgt tcgaagagga
    62161 tacggaagtg cttccgtgcc cctccttcac cgagctcttc aacgcggtcc gggaaggtcg
    62221 ggcgctctac gggatggttc ccatcgagaa cacccttggc ggaacgatct cggagaacct
    62281 cgaccagctc gaacgaaacg atgagatcga agtggtcggc gagcagcaga tcaggatcgt
    62341 ccacaacctc atcggcctgg agggaacgaa gctcgaaacc atcactcaca tctactccca
    62401 cccccaaggt cttgcccagt gcacgaggtt cctcaccgag acgatggccc acgctcaagc
    62461 ggttccgttc ttcgataccg ccggggcggt cgcgtacgtg aagagagaga acgatccgag
    62521 caaagcggcg atcgccggcg aagtcgccgc ccatgtgtat gagatggcga ttctggccga
    62581 aggaatcgaa tcgcacgccc ggaactacac ccggttctac gtcatttccc gcaaggagaa
    62641 cgccgaggcg ttcaagacgc ttcgcccgat caaccgcgcc tcgctcctct ttaccgtcgg
    62701 cgaccgtccg ggagcgctct ttgaagcact gttgattctt gcaaagcacc agttgaacat
    62761 gaagcgcctt gaatcgcgcc cgattccggg gaaaccgtgg gaatactcct tcttcgtcga
    62821 gacggaactg ggggacaacg gagcgttcga agcggcgctg gccgaactga aggagacctg
    62881 cctttcactc agggtattgg gaacctatgg ctcaaaccgg tgaatttaca aaagagaccc
    62941 tctttctcgg agtactcgca gcaagcgaga accttttgaa cgaggcgctg aattcgctca
    63001 tcgagaggta tggaccgatc aaaaagggaa gcgaggcaac accctttacc tttaccgact
    63061 actacaacga ggagatgggc ggggttcccc tgcgctccta ccatatcttc gaggaagcgg
    63121 tggatccgag ccggttggcc gagatcaaaa caacgaccaa tgaggtggag catgcgttca
    63181 gcatagaagg aaagaggcgg gtcaacctcg atcccggact cctcaccctc ggaagcctcg
    63241 tcctggcgac gacgaaaaac cgttcccacc gaattgcgct gaaccgcgga atctatgccg
    63301 agctcaccct gatctaccag gatcattcct accgggcgct tccgtggacc tatgccgatt
    63361 ataaaagcag ggaaacaatc atgcttttga actcgtggcg ctcctctttg aagcaaagca
    63421 aaggataacc tcccttacga ggtgtgcgcc tcagatgcat cattttcata tcgttgctcc
    63481 tcttcacttc atgtgaccac aacccgcttg ccgaacgggt tgtcagcgtt tcgatcggtg
    63541 aggagcatcc ctgggaggag gcttcctacc ttcccttgtg gtataccttg gtctatacca
    63601 cgaagaacgg gaacaagaaa atgcacctga gtagcggtgt ccgccaggcg aggatcgtgg
    63661 tcgaccgcct cggcagcgtc gccatctgcg cctacccgtt gggcggcttc gctccgctgg
    63721 gtggatttgt tcaggcggga aggagcgacc gggtcgtcct tacccggaaa gacggtcctc
    63781 tggcagagct tttagttgaa ggaaatctcc tcaacgccga agcgatcgct tcgctgaata
    63841 tggatcttct tgccgcgttg gtgggcgagg cggtcaatct cgatggttcc gccctgttgg
    63901 tggatctgct cagcggcatt actccaaaga agagccttca gcggcttgaa cggacgtggt
    63961 ataccctcag cggcattccc gatgggtact gggtgtgcga aaacccctct caaagctcat
    64021 tttggatcca ttttggcaaa gaggttccgc ttctcctcga tcacggtgcc acctgctgga
    64081 tgaacaggga acgctccctc atcctgacgc tcgtttcgga caacaccacg aagatggtgt
    64141 tttccgccct cggcgatgcc cccaggtggt gatcggggag tgaaagtatt tcaccatttg
    64201 ctcatccgtg tcatactata agcaatgaca acacggcgga tacatcctcc ctctcgtcat
    64261 cgacttgcag caatggtggt ggtgcttttg gctggaggtg tgttgtttgg cgcctatatc
    64321 cgggatgatc ttcgctatga agggttcttc ttccacgctt ccgaagagcc tcggacgaat
    64381 gatgaatcca ccactatcgc aatcggggag atcatcgtga tcgtcaatcg gaccagcgcc
    64441 gggcgaatca gcatctggac gacggatctg agcgatggcc ggatctccca ctcctatacg
    64501 atctgcacgt caagcggtct ggtgtgcgcc ggaaaggata cgttccacta tcagcaaaac
    64561 gaggacagcc cccacataga gatcggaacc ctctgggcga tccttcccgt ttcttatatc
    64621 gcccttgaag ggttccattc ctccatcata ttggagctcg tccttcacta gcgttatttc
    64681 accacgatat tcaccagctt gtcggggacg acgatcttct tgacgatcgt cttgccctcc
    64741 agccaggatc ggatcttctc atcatccctg gcaaggctta agagggtttc cttcgatgca
    64801 cccttttcaa ccgtcgttct ggagcggacc tttccgttga
//
LOCUS       DAOSLI010000004        62975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_540_length_62975_cov_24.812746, whole genome shotgun sequence.
ACCESSION   DAOSLI010000004 DAOSLI010000000
VERSION     DAOSLI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 62975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62975
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_540_length_62975_cov_24.812746"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..>3671)
                     /gene="cas9"
                     /locus_tag="PLF67_01245"
     CDS             complement(180..>3671)
                     /gene="cas9"
                     /locus_tag="PLF67_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOE88669.1"
                     /translation="AEEIRARLKGRPYSMGLDLGVGSIGLAIAAMEESNGYGHEPTDL
                     VFVTSRIFTPSIGASERREKRGQRNALRHRRNRLIFLWKLLAEKGLMLPYSTKSVDDP
                     ARLRFEEAMIQADPYKLRLKGLTEALTLSELGYALYHIANHRGASSIRTFLDEVQDAD
                     EKKLEEQRSITNKIMTEHNVSTFIEVLTIFNRDTFVGYRNVDKLKDKSIPVPTRDIIS
                     AELDRLLSTQARFHPDTLNEDYIQRIKDAVLYENEKIVPEPGDCPYFPDEKKLPRCHF
                     LNEERRLWEAINNARLGEPDESGSFRRYNRISFSDKERQELFTYLRSGRSVTAATVKT
                     LFPHYDNHEIILQGRDHKTSRIEGFRFKHLEEKPFWARFTEEQQDEFFAIWTNFADEK
                     RVEEYLVMHLGLSEEEAADAIRTVKLIGDYGPLGKTATLLLMKYIEDGLTFIEAEMKA
                     LETGEFQERTSREVQEFLPYYGRILSKTTQALMGKYWHSSFKDRIDRPGFVKPNTDRE
                     EEVHGRIANPVVHQTLNEMRKLVNEVIEILGSKPSEIVVELARDLKVGAEKREEISKE
                     QQRNEKRAQDIYDTYCKENGLSKRYIQAFRLYEDQQFICPYCLETISVAEIAGNQTDI
                     DHIFPIEDTSDNSYANKVLAHGSCNKEKGKRTPYAAFGHTEKWGRIMHYLDATPAMKA
                     KRWRFEKTDAEYAEYLQSKGFMSRFATDNSYIATAAFEYLACLFDYSERNSVRTTKGW
                     ETSILRKAWNLHGIDDYFGSMHWDKKDSPDSVKGKNRGDHRHHGLDAMVSLYTSRSLI
                     KQINTLSSKGMKAEEIESLIPIPNYYRDDSLPREDQRNLFRKYVQSFFERNAFVSVKT
                     NHAINGPLVKDTVYSILGANPKGDELVFVVNKKVKDIQVKKGTVEEVESAIKGKFSDK
                     TWYDDELRMKIEAIQRTNEEIIDRYKSNLVQAERTLIEENRKLEEEGKKQRTINENLI
                     SKKALELSGGAYQLLSNNSRVKTFVAKEPTTTSKGYGFDTGANLCLDLYHDEEGKLQG
                     EIIRKVYAMDPNYIPTYKQKGYKLFERIYGGDTLEITASSPIAEEKEDAIQKIARIRV
                     PNAVDGRTYIRVETFTERASGIQIFLSNISKADGGQDASFRITSMQQYDVRKVQLSPA
                     GLVEYVSPVLKDLEMGG"
     assembly_gap    3672..3771
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3977..4348
                     /locus_tag="PLF67_01250"
     CDS             3977..4348
                     /locus_tag="PLF67_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88670.1"
                     /translation="MARSSNMRKVLFVILLSLLLGSCASSRATRVGASFKESEILLNL
                     SSAAFLGHGYVAGDWVGVEADNILIRARVSDEPSDVYPTLLVGGEHVILHIPAQSAVL
                     GPYKKDINGSIHVGGTFVFTL"
     gene            complement(4338..4793)
                     /locus_tag="PLF67_01255"
     CDS             complement(4338..4793)
                     /locus_tag="PLF67_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88671.1"
                     /translation="MIFLEYETLEPIDALLWHAYPKHESIMETYDVGELTVEVLDHPS
                     LRSSIDLAVIAFSLLVFHNNEIIAVFQIEQEDLRSLSEKLGCSIRELQDEYRTKGMLS
                     NPRVYIYTKEQRKDEGPYEEELTFFSAREFLLELMCDTFDLLSDPVLRG"
     gene            4878..5696
                     /locus_tag="PLF67_01260"
     CDS             4878..5696
                     /locus_tag="PLF67_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOE88672.1"
                     /translation="MLKHPIILAFLATMGTWGMTAIGAAFVFFFKTINRHVMNGMLGF
                     ASGVMIAASFWSLLQPAIELAEGGSLPPWAVAAIGFLAGGAFLWLSDQLIPHAHLDSK
                     PGESEGIPTNLRRSILLVLAITLHNIPEGLAVGVAIGAAAAGSGSQTMTGALIVAIGI
                     GLQNLPEGAAVSVPLRREGLSRRKSFFYGQASGVVEPIAGVLGAWLVTTMTPILPYAL
                     AFAAGAMIYVVIEELIPESQQVYGTADDHSATHWATIGTMLGFTIMMILDVALG"
     gene            complement(5693..6925)
                     /locus_tag="PLF67_01265"
     CDS             complement(5693..6925)
                     /locus_tag="PLF67_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOE88673.1"
                     /translation="MGKFKSAVQRFGEFLGVAFRQSMKDNVLNSASGLVYSTLLAIVP
                     ALTFLFSFFSGLGVLEPLLNFLSEALTELTGGQTSEQVMTALTTYTRNATTLGIVGLI
                     SFLFTMVLLINKVWSVINQIYRSAQSTNPFKRFMGYMTFVIITTLLLALYVSVQSVFT
                     DWYLNLIGYSVGAWSVVIKKVIPVLIIFIIFFLLIFFVPNTKVQFSAALIGSVSGVVI
                     ITVFTQFTGLLTSRAATFSVIYGSFAALFMFLFICYVFWATIFFSVELAYVYQYRPTG
                     VVSEGLPQSPATQMTDGTNIMMVIGANFRDGKGATTVKELLDRLVIPYDRLQGFLGAL
                     TNQGYIVATNNSRTSFIPNKPLDQIRLQDLVEHLYGIETIDEMSRDTAGEAVAKQLAE
                     KGISSLGTLTVENLLQRI"
     gene            complement(6930..7850)
                     /locus_tag="PLF67_01270"
     CDS             complement(6930..7850)
                     /locus_tag="PLF67_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOE88674.1"
                     /translation="MPQLKTMISSDQTPISYHRWLPKEGAVKALLLILHGMAEHSKRY
                     DEFAKSLTKQGFAVWAPDHRGHGKTAEGKTLGFFAEEDGWQRVADDAFELSELINNEY
                     PDQPLFLLGHSMGSFLARTLLVQHSDVFDGVVIVGTSQGKGLLGKAGKMIAGRNVKKY
                     GPKHPDELLDKMSFGSYNKRIKNPVSKFDWLSRDPEQVKRYEDDPLCGYVCTSSFFKD
                     LLTGIEIANDPNLAKQLPGDVPLYIISGSDDPVGDYSKGVRSVYDFYKKAGVKDVTLT
                     LVEGGRHEVLNETDRAKTYKLIAQWMKKRI"
     gene            7961..8467
                     /locus_tag="PLF67_01275"
     CDS             7961..8467
                     /locus_tag="PLF67_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOE88675.1"
                     /translation="MLLITADIHGSTEALKEIVDIAASRKVEQVLIAGDLCPQEGSVY
                     ASLLSRLPGLVLVRGNSDASYQFAQARIHLPPLVRTLSYQGLPITLTHGHLDVPYTVG
                     GIVISGHTHIPHLKKDADGTLWLNPGSPSRPRSSSGATYALIDTEGVGIHRLKTHSPF
                     LYHRFKSS"
     gene            complement(8443..10137)
                     /locus_tag="PLF67_01280"
     CDS             complement(8443..10137)
                     /locus_tag="PLF67_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOE88676.1"
                     /translation="MKLPKMRRRKNRSFAYRGRRLLHIDLEKETSHLIELSEEESNQL
                     IGGRLLALSLWERFVSFDKLEPSFFEAHNPLVLAPALAADLEPESCGSMTLVTKDPQR
                     GKLAVSTVQTSFSTALLNCGYSAVVFTGRCRRLTGVTLDHDQVEFFDAESFHDLPTGE
                     TKHRAKIEHILCIGPAAERQIPHCSICINNQNIPFSSALGLVMGLKNVKLLGLVPHVS
                     GREGYDPGRLGIFKKRLEQSLRFSALAQRVHEEGDVRLLEKANTFGWAAIGTHRYRYD
                     LRLVHLYERFDGRADTYSLIEALALGANLEIFEPSRIAQLARRCKENGLDPASVGVLL
                     GWARVARKEGTLSFLPDLQSRLVANYLKVLDAISYGKGSGHQLGKRLDQLVAEYGGKN
                     GCYCIGSQPLGPFDYRALPTQALLASMGDHRIVLGELIRGGHYRRGKAKRSAAWALYL
                     QRLWGAQESVGLSPYVDLPFFGRWYPHIKGRRSRFAMSALIASISEGRTFTAQHLASA
                     GKRAWSLEREINRRLGWQDPPFAEQQLLDGRSNFPKSQVVPLALLRAAYHELLKRW"
     gene            10462..10797
                     /locus_tag="PLF67_01285"
     CDS             10462..10797
                     /locus_tag="PLF67_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014654251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOE88677.1"
                     /translation="MNEYIKKIYVPMTESAFFILLCLQKENHGYGIVKRVEELTDGEV
                     ILAPGTMYGSLSKMEKDGIIRFVRNEDKRKIYIITDLGLNVLKTELKRIERLYHISRE
                     VPENDEEGV"
     gene            10778..11314
                     /locus_tag="PLF67_01290"
     CDS             10778..11314
                     /locus_tag="PLF67_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOE88678.1"
                     /translation="MTKKVFKFFSITQHVKEQEYLRSMHKLGWKLVKTTGIGMYHFEA
                     CEPEDVIYQLDYNAEGLANKDEYVRMFEDCGWEYIQEFVGYSYFRKPAAKGTAKDEEI
                     FCDDESRAQMMERVFKGRLIPLLVIFFVVFIPQFFMNVTTRKSLGIAITLGAILLYMS
                     IFVSFTVQYVKLRNRMKK"
     gene            complement(11390..12010)
                     /locus_tag="PLF67_01295"
     CDS             complement(11390..12010)
                     /locus_tag="PLF67_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE88679.1"
                     /translation="MRILLVDDDMLVLKALEMILKAEGIEEVHTAKNRKEAFDIYKNH
                     RIDLVLQDIRLKDENGIDIASKLLEYDKEAKIILLTTFKDEEYINKAIKIGVKGYILK
                     DNIDSLFQSIQTVMAGNMVLDSEVIKDIRLSNRQKKSIEDFDITERELDIIKLLAEGM
                     NNKEIAQSLFLSEGTVRNYISNLLDKLYLRDRSQIVVFYYKNLHGE"
     gene            complement(12007..13047)
                     /locus_tag="PLF67_01300"
     CDS             complement(12007..13047)
                     /locus_tag="PLF67_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOE88680.1"
                     /translation="MSTITLHGIVALIISIYEFEKGDIPTESLVLLVLLGFSYSLLIK
                     VVSNQKAKTGFFLFGILAAALFPRFIFLLPLLAPSAFSQFNKVSLLLFLPFLGHFHVL
                     YLTLVGFTLLWDYLFASKEAEVVKLRKEKYNLLDDVNRLSRSIDLMKIEKERDETLTL
                     MEERNRISREIHDTAGHTLSAAILNMKALSMTTGEPDTKEHLLVLKETLENGLQDIRR
                     VMYDLRDSSFDLENKIMELLETVENSNLTYIVDLNLSYGVKYDIFAIVRESLTNYIKH
                     SDGGHFKVYLVESGRFLVLKIEDDGSTSNKEVQEGLGLYSIRKKCEERGWKFNITTDR
                     GFGIHVLMEKGI"
     gene            13142..14074
                     /locus_tag="PLF67_01305"
     CDS             13142..14074
                     /locus_tag="PLF67_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE88681.1"
                     /translation="MVIKVENLIKEFKEIRALNSFNLELKEGEILGLIGPNGSGKSTL
                     LLSVLGIYSYENGNISVFGQELKPNSYEIKRKIGYVPQDLALFEELTVYENIDYFCGL
                     YVHDRKKRKELVEKAIGFVGLEKFRKFVPSKLSGGLARRLNIACGIAHEPELIFLDEP
                     TVAVDAQSRSFILEGIKELNRRGATVIYTSHYLEEVEDLCDRIAIIDQGRNIITGTAE
                     ELRDSIITTEIIKITLLEEHVDILKDLRNLSHVIDVTIKDSEYTVKFDNNKGNIGDVA
                     QFLKEKGLTYVKLYTEVPTLNDVFLELTGKELRD"
     gene            14074..15174
                     /locus_tag="PLF67_01310"
     CDS             14074..15174
                     /locus_tag="PLF67_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88682.1"
                     /translation="MIRILTYIKYELKVFLGNYFNMFWMMGFPLVLLTIFTIFFAQLR
                     FREIEVEKVTIAYHENAFIPLSFVDEPKKRPDFDTIDRSSIETRLFEGAMLEKDQGLE
                     ELRNNRIDAYVDKDLNLFINKNGIGQLIMDEVLTTTKQISSLWLPFDAYDFERTYVKS
                     SDEKQSPIDVIFYALFSMISLYGAYLSSTIGNRLIAGRQTVALRNASSPTSKAWQVMI
                     SVVTSYLWSLFLVAVIVVYLEFILKQHFFSPDLNNIPLVLAALTFGIAWGLFLGTVIE
                     KESLQTAATISSLLILSGISGLYSHGIRLIIKRSFPLLLKLNPVSLISDELLKVNMLG
                     NYSTLKGSILLLTGYSIVLVIVSILHIRRKKA"
     gene            15171..16283
                     /locus_tag="PLF67_01315"
     CDS             15171..16283
                     /locus_tag="PLF67_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88683.1"
                     /translation="MNTLQYFIKIANKNKILIIAYIAILIAFTSLSMSSYEDAYSKRP
                     LNIGIVKQEDSQFGNSLIEHLGKGNTLYHYDSQELAELDLYTRFIDGIVVVPVDSERI
                     LLETDDSPLSIRTDVTNSQSIFLQRAVETYALYYKAMVDAGKLDLHKLSTVLDERVEV
                     VFSTDRSPLEKKFHGFANVYGFVIMMILFRLLGDLSISFNKKDIQIRNRISPKSDARL
                     KGEISLAQMIIALLVFVLVTGFILTVFLPEMLRSRSLGFYLLILFLWTAVVALLSGLI
                     NQLSKTRSLNLTIGNMLPLIIMFLSGSMLPIQFMPVFMQRIARFSPLFYYNKGIMSIS
                     EGDFDIGGELLIIAAFGLAFYLSSLYLSKERKTRTL"
     gene            16286..17224
                     /locus_tag="PLF67_01320"
     CDS             16286..17224
                     /locus_tag="PLF67_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE88684.1"
                     /translation="MKTISRGIRILLIVFLLALFIYNRLMYIKEEPLRRPLGQLVEVD
                     GRNMSVYTEGKGERTLVFMAASAVAAPILEYKPLYTLLGDDYRIVVIEKFGYGFSDVV
                     DGERSFETMLRQDREALSKAGIEGPFILCPHSMSGIEAILWAQMYPDEVEAIIGLDMS
                     VPGAYGKEDFSWKRHLLAYLLAAGREIGIVRLFFTDSTFSDALTHHEKDIYRAVASSN
                     YCNKNVRSEGKHIPKAIDLINSEPKPDLPMLMFISDGSETGGESWIEAQKEYASDLTD
                     AQTIDLDCGHMIHKYRQEQITKEMRAFIESLNGLER"
     gene            complement(17238..17444)
                     /locus_tag="PLF67_01325"
     CDS             complement(17238..17444)
                     /locus_tag="PLF67_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88685.1"
                     /translation="MMLQQKVSFFFNARKRLHTEYPFKQRPETILWMSIEEAVFSGFD
                     GGKGTQDQDLRIGPKTGMSGWVMV"
     gene            17766..18203
                     /locus_tag="PLF67_01330"
     CDS             17766..18203
                     /locus_tag="PLF67_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE88686.1"
                     /translation="MNKAVTKQMRQSLDYTLSDRVFYRQLMLINREFVMMPEFDRQWK
                     HLGLGDEELRQLQEKLLENPKAGAVLRGTGGLRKYRIAFPGRGKSGGGRVAYVDFTVH
                     ETIYLITAYPKSEKDNLTDKERNEIAKVIAVLEKGLQKQEISK"
     gene            18200..18508
                     /locus_tag="PLF67_01335"
     CDS             18200..18508
                     /locus_tag="PLF67_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE88687.1"
                     /translation="MSVYKSIMQGLDEAVKYQEGKIDARKTKITVKQVESFSSEDIKQ
                     IRKQVGLSQIVFASSLGVSKKTVEAWERGRNTPAGPSRRLLQLIRDNPEVIEQYMIKA
                     "
     gene            complement(18937..19671)
                     /locus_tag="PLF67_01340"
     CDS             complement(18937..19671)
                     /locus_tag="PLF67_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE88688.1"
                     /translation="MYKLLIADDERKIREGLALLFPWSQFDMEVVGQCSNGQEVIEFL
                     EDNEVDIVLLDIVMPKMTGIEVSKYIHENRLGIKVVLLSAFREFEYAQESLRYQVKDY
                     VVKPTKYEELVRVFTRLKRELDEQARAALDMNVPEESGIVERIKRYIEEHYATACLDS
                     IADELNLNVFYISRVFKRKTGMNIFPYLTQVRMSKACEYLQLPDIKIYEVCSLVGYTE
                     PRSFSRAFKKQFGVTPTEFRAGFGLG"
     gene            complement(19709..20974)
                     /locus_tag="PLF67_01345"
     CDS             complement(19709..20974)
                     /locus_tag="PLF67_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE88689.1"
                     /translation="MKTIKLAVIGCGNRGTVYAGYSLLHPDAARVFYACDSSKDRLEA
                     FGAMFSLPPDRLYTDASEMLEAVEDVDLVIVSTNDDSHFAIAKEVLKRKHHCLLEKPM
                     SPHAWECVELVRLAEQNGVSLILGHVLRYTPVFSTIHQLVHAERAIGDLVAIHHSENV
                     SVDHLVHSYVRGNFRNLSPILLAKSCHDLDILCWLTGRRCKKVSSFGHLDFFTEEHAP
                     EGSTYRCLDCPVEATCPFSAVKRYLGSDTGWPTSMISVDTSLEARTQALRETNYGVCA
                     FRSDNTVANHQVVNLEFEDRITVSFVLSGFNTVETREIRLMGTKGDIFANMEENYIRV
                     RTFGSHEERVIRPPVIHVGSHSGGDFLLMQDVVTRLQNNDIHQARAQASLSLESHLIA
                     FAAEHARATDTVVLLEDFTRKISNQTIGK"
     gene            complement(20991..21869)
                     /locus_tag="PLF67_01350"
     CDS             complement(20991..21869)
                     /locus_tag="PLF67_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE88690.1"
                     /translation="MNTPATVLRIPQITKRQWLRLLTHVFLIFGCFIMVFPLIWLVSS
                     AFKPGNEIFQREFSLIGSRLTVQNFIDGWNINPTYYFGHFMMNTFLMVGAVVIGTIAS
                     SFVTAYAFARLQFKGRKPLFAFMLSTMMLPAQVTLIPKYLTFTAFGWINSYLPFIVPA
                     FCATQSFHIYLLVQFIRGIPKELDSAAKIDGCGCFGIAFRIIAPLSKPAMFSVGIFAF
                     MWTWDDYLNQLIYISSVDKFTVSLFLRSLMDITSSVAWGPLLACSLISLIPIIVMFFL
                     AQPYFVQGIATSGLKG"
     gene            complement(21880..22779)
                     /locus_tag="PLF67_01355"
     CDS             complement(21880..22779)
                     /locus_tag="PLF67_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE88691.1"
                     /translation="MKAFRVKRRVFEEEGWRAYALLLPWIAGVLLFTLWPMLQSLFLS
                     FADYRIYGKASFVGLENFREMFFEDRMFKTSVFLTFKYVLLFVPIKLVAALLVAQVLN
                     RNIKGISLYRTIYYLPSIVGSSVAMAVTWKMVLSREGFVNAVLGYLHLGPIDFLSSPS
                     YALETLAMMGAWQFGSSMVIFLAGLKQVPQELYEAAEVDGTKKTRQFFSITLPMISST
                     ILFNLVMGLINAFQVFTQIAVITNGGPADSTYVYMLHLYRTAFVSHRMGYSSALAWVL
                     TLIILASTGLVFLSSKRWVYYQE"
     gene            complement(22791..24113)
                     /locus_tag="PLF67_01360"
     CDS             complement(22791..24113)
                     /locus_tag="PLF67_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOE88692.1"
                     /translation="MKKKGVILILLVVMLGMLIGCGKKPVESTSVSVAAREATKEVRF
                     MWWGSDARHAATVEAMKLFESRNPNVKLQAEYSGFSGYFDKLTTQLSAKTGPDLVQLS
                     YTNVADYVTFGQLLPLDPFIEKGIISVDNLSEATLDMYRFDGKLYAIPAGVSTTLLFY
                     NKDMFDAAGVAYPNESWTWNEYIEAAKKLTRDTNGDGKVDVWGTGEMAKVAGVDMSFK
                     KYLYERNGSLWTDDLSKVAFNSEAGVAALEFIRSFQTMGITPPIEMTAANPDGVDDFQ
                     LGRVAMIIASSPNTMMYQEAVPGLGIERVPAGIEKDVFWVNPSMMYSITKDAKDPELT
                     ASLLNFMVNDEEAGSILKVVRGTPSNSVIRANIGRSLSPTEQTMLACIQKTTSTDFVN
                     EPFPAGFMEIFTLMTREIQNYLFGKYKSAQETLSILEAESNKILARYI"
     gene            complement(24262..25995)
                     /locus_tag="PLF67_01365"
     CDS             complement(24262..25995)
                     /locus_tag="PLF67_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOE88693.1"
                     /translation="MLNAIENKHKLLTNYLKLAVPILAVMLVLGIVIIIISSSFLLTE
                     IQNNNMNVARHILDHASAIFNEIHVLSLNFGNHSLIKSGQLELLQEEGYSSERKAVEQ
                     EFLYYLQNHTNSRPYIHSGYVAFFETSDRFLVSNLGVQDIHSYYDTNWIDTAMSLQNK
                     RNPEALTVRRTTRFFSFEENQEIITVFRIIRSGIHPVGVVVLNIKPSYFDSLMRRLLL
                     VEGQTIEIYNEQGETIYSYSKGAMRENGDYLKVVEKGSMFSSVSMTPYAQLFKLPVQL
                     SKILLLVIFLLAILVLVIAYLDVRWNTRRLEVISGILDAAAKGETIPRRIVRREDEYG
                     YLIEEMIRLFVASEETRIQLIEKKSKFNALELMALQMQINPHFLFNTLETINWEASRL
                     AGSLQNTISSMVQNLSDVLKYSLSNPSESVTLKQELYYAQCYVAIQQQRYSDTIEVLW
                     DIDREALSSKGMKLLLQPLIENAIYHGAKEKEALSTITIQVRRLDDFISLRISDTGTG
                     MKPEQLAMVRLRLEEEGMEESHIGLKNVYKRLKLHYGDSVQFTIESEWRAGTSITILI
                     PFIPMDDGHNS"
     gene            26112..26606
                     /locus_tag="PLF67_01370"
     CDS             26112..26606
                     /locus_tag="PLF67_01370"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HOE88694.1"
                     /translation="MANHQTITHPLMPVFQADSKILILGSFPSVKTREYGFFYGHPQN
                     RFWPLLERIFGVKPLAGIAAKRAFLLQHHLALYDVIYRCDIIGSGDASIQNVIPSDLS
                     PILKEADIRHIFCNGGTAYRSYQKYQERKTGCKAIQLPSTSPANARYSMDDLYDHWKI
                     LREC"
     gene            26730..27488
                     /locus_tag="PLF67_01375"
     CDS             26730..27488
                     /locus_tag="PLF67_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE88695.1"
                     /translation="MDFRNKVAVVTGGAKGIGKCICDEFEKAGATVCVIDVQDNRYFK
                     GDLADKPTLERFADKVISEYGRVDYLINNAAPLSTGIDSATYEDFEYALKVGVLAPFY
                     LAKLFKPHFADSASIINISSSRDRMSQMQTESYTAAKGGIAALTHALAISLAGKVRVN
                     SISPGWIDTTDAIHEGPDAVQHPAGRVGNPMDIANTVLFLCSDKAGFITGENICIDGG
                     MTKQMIYHGDHGWTLGWRKPCMGMRKQLEHERVY"
     gene            complement(27585..29681)
                     /locus_tag="PLF67_01380"
     CDS             complement(27585..29681)
                     /locus_tag="PLF67_01380"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOE88696.1"
                     /translation="MEPNIMAAIYGLSAVTIVAAFLYALYLSRWVKAQKNENTTIERI
                     AGLIRSGANAFMKKEYRILALFSSIVAVLIFIFLPKPVWTSGTVGMNAVMAIVYLFGT
                     VFSAIAGKVGIYVASLANARTAEAARHGIKPAFRIAFRGGAVMGLLVVGACLLGVMGV
                     MLLIGNPTVLLSFSFGASSLALFAKAGGGIFTKTADVSADLVGKVELNIPEDDPRNPA
                     VIADNVGDNVGDVAGMGADLFDSNVASMSAALVMATMLDGGQGANVMMVFCYAALGLL
                     ASIVGIFTAKVGDHGSPTKALNSSTYVTTGVFLVLTALATFIIPGFQWRVWGASTAGL
                     LVGVIIGLATNYFTDDNRPIVKKVAHASKTGPAFTILSGVSYGFLSVLPAMVGIALSA
                     LIAYTLCEPLGSGYAMYGISMAAVGMLSIVGMIISNDAYGPIVDNARGLAEMGGLGEE
                     AIRAADELDSAGNTVKAVTKGFAIGAAGLTVIALLGAFMSEVNEALAQMGRAGEVLTG
                     FNLIDPTVFFGILVGAAIPAVFSAMLILGVDKNAQRMIGEIHRQFREIKGLRDGAPGV
                     EPDYNRCIDIATTGALKELIPAGLMAIIATVLVGLVGGVSAVGGFLLGNIVTGLLLAL
                     FMSNAGGLWDNAKKYIESGNEGGKGSEAHKAAVIGDTVGDPFKDTAGPSINTQITVVS
                     LVASLMATIFLNFSIM"
     gene            29922..31607
                     /locus_tag="PLF67_01385"
     CDS             29922..31607
                     /locus_tag="PLF67_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOE88697.1"
                     /translation="MRNITVSIGSEHYKVAIGTTVEEVLVIANLVTDCKAQTYKDNPY
                     IGALLNGELHSCTRSLLADCTLEPVHLFSDMGKRVYRHSISYLLCAAVSMLYPHRRLI
                     IGHALGDGYYFRFDDKLTLGIDDISTIEKTMRMLVKQDLPIEEVTMTYRAALAYFEQN
                     NFNHTVSLLSTRNDPKITLYRLQGYLDISYEPLLSRTCQMEVWELIPYQDRGMLLRYP
                     RTHNVMGLDPFVDNPKLFSVFREYKRWGSILNVQSLGQLNKLGNAHKIESFIRLAEAL
                     QQKKIVNTADMIAERSGVKAILIAGPSSSGKTTFAHKLAIQLQVLGKKTIQISMDNYY
                     LSPDRVPKDEFGKPDLEAFEALDIASFQEDLYALMNGEEVHLPRYDFKTQIRSFEKEP
                     CRLDNRTVLIIEGIHGMNPGLTNSIESESLFKIYISALTQINLDDHNRISTTDNRILR
                     RIVRDHRTRGTDAETTLNMWPSVHRGEDRYIFPYQSNADVMINSALDYELGVLVTYAQ
                     PLLRMVKPSAGPAYETARRLLRFLEHANPIPDTLVPSDSLLREFIGGSEFGVI"
     gene            31617..>32476
                     /locus_tag="PLF67_01390"
     CDS             31617..>32476
                     /locus_tag="PLF67_01390"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOE88698.1"
                     /translation="MEVYGVKKIRKSDIERALGTAVTLYKESVTSLGECTLALVRNGK
                     KKYLIAKGSGPMFDELEGEVTDDLKICPANHANRLVLNTYLPYTKPTTNKDGRPSIGL
                     GDRLGEATPGHIKALGNKNIFPYFAQQSIRELNLTGRTFDGVIDDAAYAVFQCGYTAG
                     WGADGDHLKKEEEIKTALRCGATMITLDSSEMIDNTIAGLPEKELLVRYGNVDEKTRT
                     FYENLYKERTFTFGTLSLTLDTVSLMKDILIYGKALDYIQKIWETFPEFKADEAFLEV
                     SIDETATPTDP"
     assembly_gap    32477..32576
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32577..32974
                     /locus_tag="PLF67_01395"
     CDS             <32577..32974
                     /locus_tag="PLF67_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOE88699.1"
                     /translation="RPFHVKTAGTNWLEAMHVVALTDPSLYRRMHTHALARFKDATAF
                     YVVTTDLSKIKPLDKVSDQQLCDYLKDDNARQLLHITYGYLLQDKDEKGAYLFRDEFF
                     ALLGKEEELYQDLLAKHIGKHFELLGWKK"
     gene            complement(33043..33651)
                     /locus_tag="PLF67_01400"
     CDS             complement(33043..33651)
                     /locus_tag="PLF67_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOE88700.1"
                     /translation="MSNFMAQWVYSPAVKDHFMNPRNTWDEKENFVPDGVGDVGSLAC
                     GDQMRVMIQVENDRIKAIRWLTYGCASAIASTSMLSEMAIGLTLEDAYHLSPEMITEA
                     LGGLPEHKFHCSVLGDKGLRAAIDDYLKKNGLPNPYRHSVAKIVCECHNITDVQIENL
                     VKTKQATTLEELQEITKYGTTCGKCKADIAGLFDELKHIYNV"
     gene            complement(33648..34802)
                     /locus_tag="PLF67_01405"
     CDS             complement(33648..34802)
                     /locus_tag="PLF67_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOE88701.1"
                     /translation="MRFVYLDNNATTPLHEDVIASMNEALALYGNASSMHAFGRDAAF
                     KIEEARSAVADVIGAPSGSIVFTSGASEANNTVFNIFRERIDLGSKRNRIITTEIEHP
                     SIIETVAYLRGRGYRIDLCPVDNTGRVRLDVLEELLAEDVALVSVMFGNNEIGTIQPI
                     KEIVEMAHKVGAYVHSDATQAIGKIEVSMKELGLDYLSLSAHKFYGPKGVGALAVAKG
                     APFTPYIHGGHQESGRRAGTYNTVAIIGMGVAAGIAKRDRKDEEQRLWKMREMLRKGI
                     IERIPNVVINGNQEHCLPGTLDVSFPHAEGESILLYLDLEGIQVSTGSACASGSLEPS
                     YVLLSSGVDIELAHGSIRFSFGRYNTEDDVAYVLEKLPPIIKRLREMSAL"
     gene            complement(34847..35197)
                     /locus_tag="PLF67_01410"
     CDS             complement(34847..35197)
                     /locus_tag="PLF67_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88702.1"
                     /translation="MGAKWESKCSRCGLCCHEKVVSGNTLVIDLDSRCEFYDPKSKQC
                     TVYAERFVESKRCLKMTVWRAMFASYIPETCAYAQWARTGRIRFARKRYLRIIHTKTC
                     PAEDGEDPFYSAFG"
     gene            complement(35197..35985)
                     /gene="surE"
                     /locus_tag="PLF67_01415"
     CDS             complement(35197..35985)
                     /gene="surE"
                     /locus_tag="PLF67_01415"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOE88703.1"
                     /translation="MKILLTNDDGYTSEGLNTLADVLEKAGHDIWICAPTFERSASSH
                     AMTLREDITITEYQPNRYHCSGKPADCILYASRVGLFGEKPDVVISGINHGYNISTDV
                     LYSGTVAAAREAAINGMKAIAISAAKDEDGRFAFIEGARFVRDNLAFFMDLVDETTII
                     SINVPPRPNGKAKPANLAYLEYHDAIATKKSEEVHVVDPSSVRFGTAVILTLKGGTGP
                     TQRCATRESDFQAVQDGYISITALDVLPSINSAVQTKLASLGGG"
     gene            complement(35992..37158)
                     /locus_tag="PLF67_01420"
     CDS             complement(35992..37158)
                     /locus_tag="PLF67_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOE88704.1"
                     /translation="MEQLIAKHKEEYGHTPEVIVQVPGACTVLGAYADACRGWSLVGT
                     DRSTLYVAISVRDDNMVRLFNATLNDRKRFPLNNIKFRKEDRWGNYPKGVIAMMSADN
                     HEIPGLDITLKGRLLYADNQMVSTSSAIGTVLAVDHLLKLDLSFSATIRLVYQANTTF
                     NSEPCRVSDLLTILNSGRDKLFFFDLQHVTYQERVFPFTEESPQYVALIVDSKIPPSA
                     MREEIYYKKLDIEAAFEQLSEHYSGAFIRDFPESDITSRVVHLDEDARHNAQYVLMES
                     QLVKDAANLLTPRDAPQLGKIFGRIQTGLRDLLEVTVPEIDWLIKRAGELDGCLGAVQ
                     VDNGFAGNIMVLLSKEALPSYIDRLEEYEHIFGFKPRWHIYGANDPARVVFPDD"
     gene            complement(37334..37537)
                     /gene="rpmE"
                     /locus_tag="PLF67_01425"
     CDS             complement(37334..37537)
                     /gene="rpmE"
                     /locus_tag="PLF67_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOE88705.1"
                     /translation="MKEKIHPRYELTEIKCSCGNVIKTKTTARSMEVEICSVCHPFFT
                     GTQKVVDTAGRIERFKKRYSLEK"
     gene            complement(37603..39303)
                     /gene="rho"
                     /locus_tag="PLF67_01430"
     CDS             complement(37603..39303)
                     /gene="rho"
                     /locus_tag="PLF67_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOE88706.1"
                     /translation="MSVEEVEKTSAEVAEEVKPVKVAKRVVVKKSSEEAPKEEVKRKV
                     GRPRKKADPLPPEAAAPVEEPATPVPAPAEKPAAPVEKPRAPQRKASGRQSSRNTQNR
                     KGSYQKSQSFGPGRPSAHLNIEDTSIDLNIEEHPDAPVLELEYYATLGIDTLRKVGVE
                     RGIDEEAILDLRKQEIVAEILRRHTGEGGVVVGTGTLEILPDGFGFLRSPANSYLSGQ
                     EDVYVSPAQIKSLYLKTGDVVYGQVRTPKENERFFALLKVLKVNGDDPIQAKIRVPFD
                     NLTPLFPDERLRLETVDEDISTRIIDMFCPIGKGQRSLIVAPPRTGKTVLLQKIANSI
                     AINHPEVTLMVLLVDERPEEVTDMRRHVKGEVIASTFDEQASRHVQVAEMVIEKAKRL
                     VEHKKDVVILLDSITRLARAYNQTVPASGKILSGGVDSNALHKPKRFFGAARNIESGG
                     SLTIVATGLIETGSRMDEVIFEEFKGTGNNEIILDRRLADKRLFPAINIKKSGTRRED
                     LLLPSDEAARIWLTRNAVNDMDEQEITPFLIDKIRKTKDNEAFLRSINTGVSASGSSY
                     "
     gene            complement(39447..40712)
                     /locus_tag="PLF67_01435"
     CDS             complement(39447..40712)
                     /locus_tag="PLF67_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOE88707.1"
                     /translation="MNYTRAALFIIGTELTRGVISDRHGRLVSSQLTQLGYRVERIII
                     LPDDGTVANHLRECVKTCDVVLVTGGLGPTSDDRTRSIVADLAGVELVRDLLAFEALY
                     QRMGERIWGANEQQTMIPEGFEVIPNPNGTACGFRGFVRVDEREVLVVALPGPPREMD
                     PMFFNHVRPLLGELSGHDDFERSDYSTYLLAESKLEELCRSVAVEGLEWGTRFQEQTI
                     SLYIAGGNREKRTAMVEKLRSLVGPLLIVDGDVQPVELLIRELKERGQSIATAESITA
                     GLVSKLLTDQSGSSAWYWGGVNVYANEAKTRLLGVKEETLSTFGAVSVECAAEMAEGM
                     LNQSSVDWALSVTGIAGPGGGSEEKPVGTVCFGFASRTRETQSVQMHLSAYSRDIMRR
                     RAATVAFLLGTLYIQGGRLLDTVKKWQYS"
     gene            complement(40709..42349)
                     /gene="lnt"
                     /locus_tag="PLF67_01440"
     CDS             complement(40709..42349)
                     /gene="lnt"
                     /locus_tag="PLF67_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOE88708.1"
                     /translation="MTNSLSRRSLRTVGSEVFALIASAFVYSLAFPGFINTTGFGWIA
                     FFALIPMFMVIRNTTWKLVIPYGFFYGFVFYLFFNYWLKTFHPLAILLVPIIKGGEMI
                     ALFPALKLSQRFFKKYSYLVDAVIYVAYTYLSTNWFAGYPYGMLGSAIYRYLVLIQIA
                     EITGIWGVTFLMVLPQTFLAFFLVERKKEFFSFIKDHLIPVIGYGILVLASIIYGIVS
                     LAYWNGQTPDLTWRVATVQHSADTWKGGYTTYKNNFNNLRRLSLEALTRNPDIILWSE
                     TAFVPSVAWHENYPSDPNTRVLVDEFVAFGKSLPVPLVTGNPEGVIKNPDLPAFGADG
                     EWNRMDYNTVIFFENGKIKDTYRKQHLVPFTEHFPYEKQLPWLYRLLLANDYNWWETG
                     YESVVFTTDEGVRFSTPICFEDVFGYLNANFVRNGADVIVNMTNDGWSKAVSAQMQHL
                     GLAVFRSIENRRTTVRGTNSGMTCVIDVNGKILDMMEPFKVGWKIYDVPVYTQESHGF
                     TFYTRHIDLFAFVAIYLSYALLGFGLIRCVVTTIRKRR"
     gene            complement(42365..42685)
                     /locus_tag="PLF67_01445"
     CDS             complement(42365..42685)
                     /locus_tag="PLF67_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE88709.1"
                     /translation="MANPKLTKAMIIENLYEKHGINRADIHTIIDEFFEEIKEGLKKD
                     QVIELRGFGTFEVRTRKGREKARNPKTGDLVSVETHGVAIFRPGKELKECVWDLRSND
                     STDN"
     gene            complement(42714..42998)
                     /gene="rpsT"
                     /locus_tag="PLF67_01450"
     CDS             complement(42714..42998)
                     /gene="rpsT"
                     /locus_tag="PLF67_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOE88710.1"
                     /translation="MSINRSAEKRERQNRKRRLRNRSTKSTMKTAIRKFNEAVASGDV
                     ESAGATLATSLKLIDSTASKGVIHKNTANRKKSRLQLRFNKMEQANVAQA"
     gene            complement(43123..44136)
                     /gene="ricT"
                     /locus_tag="PLF67_01455"
     CDS             complement(43123..44136)
                     /gene="ricT"
                     /locus_tag="PLF67_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOE88711.1"
                     /translation="MKNPASNETTLCAWDSVLYPGTSVVAPTRYGLDLGVVVSNANRL
                     NQEYRGGDTNVRGCCEHAELWDEVEVAEELAGSDEIIYNDRPDEHQCFSCKGCVPSVE
                     PQIVSLHDTDVQWIDRLATPSDLARYQELCGQEDEAMRICREKIAERNLDMKLVTTHF
                     LLGEPKIIFFFTADVRVDFRELVKDLVSIFKIRIELRQVGVRDESRVLGGLAVCGRDY
                     CCHSMTDTLNPVSIKMAKEQNLSLNSMKISGPCGRLLCCLSYEYDFYNEEKQNYPPRG
                     SRLKVGSDLMKVTEVNILSKQITLSGSEGRVANLPQAALFFNDHANRWEVKREYVTEF
                     LSN"
     gene            44369..45148
                     /locus_tag="PLF67_01460"
     CDS             44369..45148
                     /locus_tag="PLF67_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HOE88712.1"
                     /translation="MKLFDTHAHIGLIQDDQMSQLLAVQMAKVKGVTHVVSITNSLAD
                     FEKTYENLKSNDSVLHAVGVSPTEVLNPGQNWEERVMANAKRDRVIAIGETGLDYHHY
                     AQARSQQIELMLKHLDIARILEKPIIIHNRDAKDDLLPILFEKLPPKGGILHCFGEDW
                     AFARKVLDLDLFFSFAGNLTFKHNRNLHEVAYRLPLDRILIESEAPFMTPHRYKGERN
                     KIHYLVETLYAIAEIRDMAVEDVSEVIYDNSFRAFALKQES"
     gene            45145..46149
                     /gene="dusB"
                     /locus_tag="PLF67_01465"
     CDS             45145..46149
                     /gene="dusB"
                     /locus_tag="PLF67_01465"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOE88713.1"
                     /translation="MTRHHPIFIADALTLKGNLFLAPLAGYTDAPFRQLCIEGGADFT
                     YTEMVSVEGLARKGEKTIALLPRAKDEKEYAIQLFLGELASLRSALAIVKEHQPTMID
                     INAGCPVPKVTKTGAGSALMKNPPLIMEIVRIIKEESGIPTSVKFRIGWDHENQNYLE
                     FADAAIDGGADLLTLHARTRAQGYTPFAQWDCLTSLKTHLAKKGLTIPLIGSGDLFEA
                     EDAVRMLRETNIDGVMFARGAVGNPVIFNQTKALLEGKEKPLLTATDKVNLLLTHLYA
                     LAGYYDELTACALMRKFIGGYLKGIPHAAKVKQAAVQALTIKAYEDALRHLALSEDRI
                     "
     gene            46279..47040
                     /locus_tag="PLF67_01470"
     CDS             46279..47040
                     /locus_tag="PLF67_01470"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOE88714.1"
                     /translation="MSGATEIYYNELMRFFGLIMQDEGDSIDKAATLLVQEIKDDRII
                     NVIGTGGHSNIGAWEMFYRAGGLRCINAILDPGTSLEPGALRSTIIERALGYGTAVLT
                     AYEVNEGVLIIVNAYGLNTMTIEVALEARKRGIKTIGVTSRTFSESVPKDHPARHPSK
                     QNLCDVVDVHIDTHMPVMDATVPIDGLLAPVGPTSTILNAFCLNMLVVRTVELCMAQG
                     IEPPVNISANVAFTPEMVEYTRRMMEKYKHRLYHY"
     gene            47055..>47251
                     /locus_tag="PLF67_01475"
     CDS             47055..>47251
                     /locus_tag="PLF67_01475"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOE88715.1"
                     /translation="MASTIRVLVFGAHPDDDDLQCGGTAIKLANAGHTVKFVSCTNGG
                     TGHHEIGGIELVRRRYEEAQRA"
     assembly_gap    47252..47351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <47352..48031
                     /locus_tag="PLF67_01480"
     CDS             <47352..48031
                     /locus_tag="PLF67_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOE88716.1"
                     /translation="EIGGIELVRRRYEEAQRAAKVAGIAEYQVLDIHNGELEASVVNR
                     KTIIRIIREFRPDLIITHRPYDYHPDHRVTSQLVQDASYIMSVPNMLPLTEAMTEFPV
                     ICYMSDTFQKPIPFSPDIVIGIDDVFDRKVEMIHCHTSQMYEWLPYNRGVLHTVPTGE
                     GKRKEWLREHFLDPRDRADRYRNRLIELYGEAEGKAIRYAEAFEVCEYGRPLQLTRTE
                     IENVFVL"
     gene            48052..48318
                     /locus_tag="PLF67_01485"
     CDS             48052..48318
                     /locus_tag="PLF67_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88717.1"
                     /translation="MSIFETIMLACFGMAWPVNIMKTVRSKTARGRSLMFQAIILVGY
                     ISGIIHKLLYNLDIVLFLYCLNFVMVGIDGALLLYYKRKEACCS"
     gene            48785..49864
                     /gene="chvE"
                     /locus_tag="PLF67_01490"
     CDS             48785..49864
                     /gene="chvE"
                     /locus_tag="PLF67_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOE88718.1"
                     /translation="MKKSMYVLLTVLLMATTLFAAGSQEQSSAIKIGVSMPTQSLQRW
                     NQDGANMKSQLEAAGYKVDLQYAGDNDIPTQVAQLENMITTGCKVLVIASIDGSSLTE
                     VLKQAQEKKISVIAYDRLIMNTDAVTYYATFDNFKVGTIQGTYIRDALKLDSTPGPYY
                     IELFTGSPDDNNINFFFGGAMSILEPYIKSGKLVVRSGQTTKAQCATPNWSTEEAQKR
                     MENLITANGYGPKGTRLDAVYSSNDSVANGITNALVAAGYTKDNFPILTGQDCDKPAV
                     KNMLQGLQSMSIFKDTRTLAAKVVEMVNSIIKGEVVSINDTTTYDNGIKVVPSFLCEP
                     VFATVDNYKALLIDSGYYTEADLKI"
     gene            49926..51470
                     /gene="gguA"
                     /locus_tag="PLF67_01495"
     CDS             49926..51470
                     /gene="gguA"
                     /locus_tag="PLF67_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE88719.1"
                     /translation="METFLLEMKHITKIFPGVRALNDVNLQVRPGEIHAIVGENGAGK
                     STLMNILSGVYPASTFEGDILVHGKSCQFHTIKESEEQGIVIIHQELALVPYLTIAEN
                     MFLGNERGRYFSIDWDKTYSRADELLSLVGLEESSKTAIKDIGVGKQQLVEIAKALAK
                     NVKVLILDEPTASLNDTEAKKLLDLLLQFKERGMTSILISHKLNEVAYVADRITVIRD
                     GEVITTMDKAKEAFDENKIINAMVGRNISDRFPKRNATIGEVSLEVEHWTVSHPLYEG
                     MKVCEDINLTLRKGEVVGLSGLMGAGRTEFAMSLFGKSYGRDITGTIKINGKEVQLNT
                     VKSAIENKLAYVTEDRKSNGLILSKSIMLNTTLANLKEVSRHQVIDPDLELKVAIDVN
                     RKLQTKCSSVLEEVNNLSGGNQQKVLLSKWIFAEPDILLLDEPTRGIDVGAKYEIYCI
                     INQLAEEGKSILFISSEMPEILGICDRIYVLSEGRIVGEMSGSEASQEKIMKCIIQNQ
                     KGDQLP"
     gene            51467..52648
                     /gene="gguB"
                     /locus_tag="PLF67_01500"
     CDS             51467..52648
                     /gene="gguB"
                     /locus_tag="PLF67_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE88720.1"
                     /translation="MRHSLGIKDTVKKNTMLIVLLATMVLFQVLISSSGRGSLFAPSN
                     ISNLINQNSYVVILASGMLLCILTGGNIDLSVGSVVALVGALAGTLVVNLHWNIYLSI
                     LICLLTGLAIGAWQGFWIAYVRIPPFITTLAGMLLWRGVALLILNGLTISPFPAEYTK
                     YFNSFLPNTGDPATVFSVTVIVGVIICLVYGFWALFDRYTKKKKGYATEPFVMTLSKV
                     ILLSIAVLVVFTLLGKHKGVPVILILLAIIVLGYSYYTSRTVPGRHLYAMGGNEKAAK
                     LSGIDTNRVLFFVYTNMGFLSAVAALVGVARFNSAAPTAGTNYEMDAIGSCFIGGASA
                     YGGTGTIGGAIIGAIFMGVLNNGMSILGIDANWQKAVKGIVVLAAVVFDVLSKKRVKS
                     N"
     gene            complement(52650..53831)
                     /gene="nagA"
                     /locus_tag="PLF67_01505"
     CDS             complement(52650..53831)
                     /gene="nagA"
                     /locus_tag="PLF67_01505"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOE88721.1"
                     /translation="MRTLIKNVRLIMPDRVMGDARLVIRDGLIDDFGTEAYPNGEFHE
                     VIDGKGAYLSPGFVDTHVHGGNGTSFHDGSEEAIRNVLSVHLKGGTTSILPTLTSLTH
                     EQYLNILSLFADLDFRGEAVPDIEGIHMEGPYCSGSVLGAQDVKTYRTVDYREVDAYL
                     EIYPKIRKWTAACELEGGMGFGRFLERKGIVASIGHSNATLQETFEAYNNGYRHVTHL
                     YSSCSSYHREGAYRKAGIVEAAFLIDGMDVEVIGDGVHLPKEFLQLIYKIKGPEHICL
                     VTDATRWGGAKLPDGTRTFADAAESRVIYIENGVALVEDKSCFAGSIATADRLVRTAT
                     KIAGIPLVDSVRMATLTPARIIGIDRYVGSIARGKRANLLLFNDDIDIEGVFIQGKRV
                     L"
     gene            complement(53836..54669)
                     /locus_tag="PLF67_01510"
     CDS             complement(53836..54669)
                     /locus_tag="PLF67_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE88722.1"
                     /translation="MARKRRLINSVLQSLALLFISLLFVLPIVFCVFTSFKESIEIIK
                     EVTFFPKKWSLVNYDYVFVRGAKYLKYYLNTIIITMSSVIVTLLLSALSGYSFAKLPF
                     KGSNALLASILFVLAFPLAAILIPIYIMEYNLGLLNTYIGLILPNLLLILPFSIFIMR
                     GTFINLSRELEDSAEIDGCSVVQTWWKIMLPLAQNALVIVFVSGFYSVWGEYTISKSL
                     ATKDSIMPISVALTLLKSEDWNYGVLAAAITMSIIPPITIFAIFQNRLVEGMVEGAIK
                     G"
     gene            complement(54671..55564)
                     /locus_tag="PLF67_01515"
     CDS             complement(54671..55564)
                     /locus_tag="PLF67_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE88723.1"
                     /translation="MQNTIRGRKREKELFGMLFVLPTVLVLFLFVVYPISYTAILSFS
                     DYNFAFDKRPKFNGFNNFLATAKDIQFVRALSNTLVFTVIMFTLLIVLSLAFALLLFY
                     KKKRSWFYRTSIFMPIVVPASLICLLFTWMLADNFGILNKFLISVGLSKYAHNWLTDA
                     KTARWWVIIVSIWGKVGFSTILFLSGLQGISSELFEAAEIDGAVGFKKLIYIIIPNLS
                     ETFAVVGIWAVLQCLKLFVTPNVLTKGGPGNATIVLYQKIYNEAFMNYEMGYASSIAF
                     ILTALVMVFSILNLKISRKEE"
     gene            complement(55637..56917)
                     /locus_tag="PLF67_01520"
     CDS             complement(55637..56917)
                     /locus_tag="PLF67_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE88724.1"
                     /translation="MKRIAIVLLVLVLAMGMVFAQGKGEKEATGRRTLRISAESWQIS
                     KIYLEKAKEIFEKNHPDVDVEIITLADQNVLANYVIDWSKGNTETDLVFLDGGYMAKT
                     YLAQGLIYDFDKELNFFKDFPKSKYQPGALETGVYEGVQVCVPAIYEVYGISLNKKMF
                     QEAGLVDANGEPLPMKTWDDFYKFSEKLTKKDAGGVVTQVGSSIQFGNNLTGIIGGAI
                     VAQKGHITKPDGYTYDFDFPEFVNMVDLWQKGVQNGYISTSTFVDNAGGRNGFKAGQI
                     AMVYEAAGRWMEAIPTIGIENLSLAPIPGGKGTFAFGCQMAIPKASKNADLAMQFIKE
                     GVYSEYVQVNTFTKFGKMSVIKEHFNKALDETPMWKKLSDSMENGVRIPQWQDQAKWL
                     TGLNVIFQEGLVDPKTSARDIVNKMTALSDSLKK"
     gene            complement(57332..58618)
                     /locus_tag="PLF67_01525"
     CDS             complement(57332..58618)
                     /locus_tag="PLF67_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88725.1"
                     /translation="MKQIIDDTMMLMLQRRTLRPDYGWIDLKLDAISGEDMFESDGIR
                     GKNHIYSWIQGRGLEAIATHLKWYDRVGWPSDDAKEELISLGRSVSANLQRQRRSNGG
                     HLYFLATDRSHPTEDTDVFTMSDLFCSRGLYAFNHDWGTEEERRASRRYITDTLRAII
                     GNKLVNDQIGFHPSQYQGRKAKATGFGTEMLGLGAVTLLMRLERDSNAPAIGRRFVEK
                     ILSKHVNSMGRWPSIKDGIIVEMLDDEGNVFLDEEGRVLVDPGHSLEFIGLAAQMVAV
                     YLECYGNEETEYEWMIDVGGTLDSLIEPNLKLGFSETGGIVKRIDAVSKTIPYDTMPW
                     WSLPETLRAIALTEHLKKGPWSTFGTEWFERCLSAFTENYRKPSACPIAVQTIDSSGK
                     PVDVIPATPDLDPGYHTGLALLDCCNVLSKQSVVLQ"
     gene            complement(58641..58880)
                     /locus_tag="PLF67_01530"
     CDS             complement(58641..58880)
                     /locus_tag="PLF67_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88726.1"
                     /translation="MERKNVLTIQTPGKKHSVYLAAGQHLRDVLHRLGLHGLSFPCGG
                     NSVGGSAISDYVGLMVEIGLINETGGCCGNNQASG"
     gene            59099..60355
                     /gene="purD"
                     /locus_tag="PLF67_01535"
     CDS             59099..60355
                     /gene="purD"
                     /locus_tag="PLF67_01535"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOE88727.1"
                     /translation="MRVLVLGSGAKDHAIAWWFSQSRLISALYVAPGNAGTESIAKNI
                     EIDPSDPEAVYRVCQTHTINYVFVGTEAPLFTGVIDYLNERGIDTFGAPKRALPLEGD
                     RDFARKFTERHRIPAPRYVLFRNTDDLSAYLKEHEGERFVVKSNQIAPSRVMVDSTDY
                     TTLMEFSATLLESGPIILEQHLAGLPLTITLFVDTNSYMLLPICSDYMRVESGGTPTG
                     GMGSICPVPLQDSVKEALISRIIEPTLNGLREEHMAYKGVLTISIILTKEGPMLVDYH
                     VRFNDPAAQAFIPLICTDLIEILTAMKKDTLGSLKLCCSSNSAVALVVASEGYPQKPV
                     TGKILNPIPAALLLNAFEGAPRYFFGGVQKRGNDLVTTGGRNVTVVGIDYNIMSANKR
                     AYQGVVHVQFENAWYRQDIGNKFFEN"
     gene            complement(60369..60641)
                     /locus_tag="PLF67_01540"
     CDS             complement(60369..60641)
                     /locus_tag="PLF67_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88728.1"
                     /translation="MNIVHPFMEGNGRSTRIWLDLILKKRLRKCIDWSKIEKRSYLDA
                     MEASVVDSHPLKILLFEALTDLIDDRAMFMKGIDYSFYYEEDAFIE"
     gene            60634..60780
                     /locus_tag="PLF67_01545"
     CDS             60634..60780
                     /locus_tag="PLF67_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88729.1"
                     /translation="MFISMYFVKGLFAHLLFLLQFLMKKLSHRELEAPLGDVTSANLS
                     GKVV"
     gene            complement(60805..60966)
                     /locus_tag="PLF67_01550"
     CDS             complement(60805..60966)
                     /locus_tag="PLF67_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88730.1"
                     /translation="MAKQFPGKQANRFLQWFTYGEESIDGLSKAKAYSLFDSSLLESI
                     EVGTVNGFQ"
     gene            61174..62781
                     /locus_tag="PLF67_01555"
     CDS             61174..62781
                     /locus_tag="PLF67_01555"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HOE88731.1"
                     /translation="MNSTAFFENGQLFLNLSIPLKLDFQNPVKALHTLLWSLDYSCFS
                     KQKKVSGRPNALSPRKMMFILVYARLNNCFSSREVEALCKRDLVCIHVLEGGRAPDHS
                     TIDRFVRGNAGPIRDIFAQSIRKLDELGELGRKMAFIDGTKIESKAGKYTFVWLSAIE
                     KNLPKLVRNIAKLYGQYVEHYRLDNPSVVETEIQAKVALDEMIEVIDPHEVLSRPCKQ
                     GRGHRKGFERMLSERLLEYSRKLAFYRRARNRMKAEGRRSMSKTDEDATFMRMKEDYM
                     RNGQLKPGYNIQNIVDSGYVVGTYCSSDRTDMYTFVPALEQLRKRLGFEYQGICADSG
                     YDCVENYRYLESRGIDAYIKTQTYEQNKKRKIRKDPGNKLNMTYDGAKDRLRCMNNAI
                     LHNTGKKKSDGTSLYEAKKGCVSCTYRNICMKGQATQKNFKRMQYNPVAEGFRSKSLS
                     NIVSEKGIEARLNRSIQVEGSFALIKDALGIRRFLNRGMVNVETEWIILCMAANTLKL
                     QAKINRGCLGIPTWYHIDKSDPLDEAG"
BASE COUNT        15375 a        16722 c        15701 g        14877 t
ORIGIN      
        1 tcagacaagc cttgaatacc gaatcggagt acagaatccc gttcccatgg caggtgatcg
       61 agtgaatgtc ctggtagggg attttcgtct ctttaccgtc ccgttcaaca accagggagt
      121 ttcgctcgga gtgcaggtag taattgtctc ccccaagatc caatactcgc cacatatccc
      181 taccctccca tttcaagatc cttcagcaca ggtgagacgt attcgaccaa gcctgccggg
      241 ctgagctgaa cttttctcac atcgtattgt tgcattgagg taatcctgaa acttgcatcc
      301 tgaccaccat ctgctttgga gatatttgag agaaatattt gaattccaga agctctttct
      361 gtgaatgtct ccacccttat ataggtacgc ccatcaaccg cattaggcac tcgtattcta
      421 gcaatctttt gtattgcatc ctctttttct tcagctatag gagaagaagc tgttatctca
      481 agcgtgtctc ctccataaat ccgctcaaac agcttgtatc ctttttgttt gtacgttggg
      541 atgtaattcg gatccatcgc atagactttt ctgatgattt cgccttgaag cttgccttct
      601 tcgtcgtggt ataagtcaag acacaagttt gcaccggtgt cgaatccgta ccctttggaa
      661 gtggttgtcg gctccttggc cacaaaggtc tttactctcg agttgttgga aagaagctga
      721 tacgctcctc cggacaactc cagggctttc ttgctgatca aattttcatt aatggtgcgc
      781 tgtttctttc cctcttcctc cagcttcctg ttctcttcga tgagggtccg ttcggcctgg
      841 acgaggtttg atttgtagcg atcgatgatt tcctcatttg ttcgctggat tgcttcaatc
      901 ttcatgcgca gttcatcatc gtaccaggtt ttgtcgctaa actttccttt gatcgcggac
      961 tcgacctcct cgacggtacc ctttttaacc tggatatcct ttaccttctt attgacgacg
     1021 aagaccagtt catcgccctt aggattcgca ccgagaatcg aatatacggt atccttcaca
     1081 agcggtccat ttatcgcatg attcgtcttg acgcttacaa acgcgttgcg ctcaaagaaa
     1141 gactggacat actttctgaa tagatttcgc tgatcctccc tgggtagcga atcatcacga
     1201 tagtagttgg gaatcggaat gaggctctca atttcctcag ctttcatccc tttggaagaa
     1261 agcgtgttta tctgcttgat caatgaacgg gaggtataca aggagaccat cgcatccaag
     1321 ccatgatggc gatgatcgcc tcggttcttc cctttcactg aatcggggga atctttcttg
     1381 tcccaatgca tgctgccgaa gtaatcatcg attccatgaa gattccaagc cttcctgagg
     1441 atgcttgtct cccaaccctt ggtagtccga acggaattgc gctcactgta gtcaaacaag
     1501 caggcaagat actcgaacgc ggcagttgcg atgtagctgt tgtcggtggc gaaacgcgac
     1561 atgaaccctt tggattgcaa atactccgca tattcagcat ccgtcttctc gaatctccac
     1621 cgcttcgcct tcatggccgg cgttgcatcg agataatgca tgatcctgcc ccatttttcc
     1681 gtatgcccga atgcggcata cggagtacgt ttacctttct ctttattgca cgagccgtgg
     1741 gcaagcacct tgtttgcata cgagttgtcc gaggtgtcct ctatcgggaa gatgtggtcg
     1801 atatccgtct gattgcccgc tatctccgct acgctgatgg tttcaaggca atacggacag
     1861 atgaactgct gatcctcata caatcggaac gcttggatat atcgtttgga aagaccgttc
     1921 tccttgcaat acgtgtcata gatgtcctga gctctttttt cattacgttg ttgctctttg
     1981 ctgatctcct cgcgcttttc agccccgacc ttgagatccc gggcaagctc aacaacgatt
     2041 tcggaaggtt tggagccaag aatctcaatg acttcattca cgagcttcct catctcgttg
     2101 agcgtttggt ggacgacggg gttggcgata cgcccatgca cctcttcttc cctatccgta
     2161 ttgggcttca cgaagcccgg cctgtcgatc cggtctttga aggaagaatg ccaatatttc
     2221 cccatcaagg cctgtgtcgt tttggaaagg attcgtccat agtacggcag gaactcctgg
     2281 acctctcggc ttgttctttc ctggaactcc ccggtttcca gagctttcat ctctgcttcg
     2341 ataaaggtaa gaccatcctc gatatacttc atcaacaaca aggtggcggt ctttcccaaa
     2401 ggcccgtaat caccgatcag cttgacggtc ctgatcgcat cggcggcctc ctcttcgctc
     2461 aggccgaggt gcatgaccag atattcttcc actctttttt catcggcgaa gttcgtccag
     2521 attgcgaaaa actcatcctg ctgctcttcg gtgaatcggg cccagaatgg tttctcttca
     2581 aggtgtttga atcgaaaccc ttcaattctg gatgtcttgt gatctctgcc ttggaggatg
     2641 atttcatgat tatcgtaatg aggaaacaag gttttgacag tggctgcggt cacacttctc
     2701 cccgatctga gataggtgaa gagctcctgt cgctccttgt cgctgaaaga aatccgattg
     2761 tatctccgga aagagcctga ctcatccggt tcgcccaagc gggcgttgtt gatggcttcc
     2821 cacagcctgc gctcttcatt gaggaagtga cagcgtggaa gcttcttctc atcaggaaag
     2881 tagggacagt cccccggctc gggcacgatc ttctcgttct cgtagaggac cgcatccttg
     2941 atccgttgga tgtaatcctc gttcaacgta tcaggatgga atcgggcttg cgtcgacagc
     3001 aaacggtcaa gctcagcgct gatgatgtcc cgagtcggaa ccgggatgga cttgtccttc
     3061 agtttgtcaa catttcgata accgacaaac gtatcccggt tgaatatggt cagcacttcg
     3121 atgaacgtgg aaacattatg ctcggtcatg atcttgttcg tgatcgaacg ctgttcttca
     3181 agttttttct catccgcgtc ttgcacttca tccaaaaatg tccggatcga ggaagcaccc
     3241 cgatgattcg cgatatggta gagcgcatat ccgagctcgc tcagcgtcaa ggcttccgtc
     3301 aaccccttga gccgaagctt gtagggatcg gcctgtatca tggcctcttc aaagcggagc
     3361 cttgcagggt catcaacgct cttggtcgaa tatggtagca tcaacccctt ctccgccaag
     3421 agcttccaca ggaagatcag gcggtttcga cggtgtcgca acgcgttccg ctgtcctctt
     3481 ttctctcttc gctcggaagc tccgattgaa ggggtgaaga tccgcgaggt gacaaacacc
     3541 agatcggtcg gctcatgacc gtagccgttc gactcttcca tcgcggcgat cgcaaggccg
     3601 atcgagccca ctcccagatc aaggcccatc gaatatggac gccctttcag tcttgctcga
     3661 atctcttccg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaaaaacac
     3781 cgtcatggca tggtttacct ctttcttcga tcctgcttcc ctagggcgag tgtccggcac
     3841 gcaatgggga tttaccatcg acaacctcct ccggtagaga aattccgagt gtcgtgatag
     3901 gatcggggca tgaagaagcg aacgatcgtc atgaacacga gcgaggaact ccgccttgcc
     3961 cgcaaggagc tgatggatgg cacgttcatc aaacatgcgt aaggttcttt tcgtgatcct
     4021 cctctccttg ctgctcggct cctgcgcctc gagccgggca acccgggttg gggcgagctt
     4081 caaagaaagc gaaatcctct tgaatctttc cagcgccgcg ttcctcggtc acggatatgt
     4141 cgcaggagat tgggtcggag tcgaagctga taacattttg attcgtgccc gggtgagcga
     4201 cgaaccctca gacgtatacc cgaccctgct tgtcggaggc gaacacgtga ttcttcatat
     4261 cccggcacag tcggccgttc tgggccccta taagaaagat atcaatggaa gcatccacgt
     4321 gggcggaacg ttcgtcttca ccctctgagg acgggatcgg aaagcaggtc aaacgtatca
     4381 cacatcagtt cgaggagaaa ctcgcgggcg gaaaagaacg tgagttcctc ttcatacggg
     4441 ccctcgtcct tccgctgctc cttcgtgtag atatataccc gggggttgct cagcattcct
     4501 ttcgtccggt attcatcctg aagctcgcgg atgctgcacc ccagtttttc agaaagggag
     4561 cgcagatcct cctgctcgat ctggaagacc gcgatgattt cattgttatg aaacacgaga
     4621 agagaaaacg cgatgacggc gagatcgatc gaggaacgga gactcggatg atcgagcacc
     4681 tcgacggtga gttctccgac atcataggtt tccataatcg actcatgctt cgggtatgca
     4741 tgccaaagca gagcgtcgat cggttcaagg gtttcgtact caaggaagat cattgttcaa
     4801 ccataatggg aagaactccc tttaggcaag agatattcct acttgaccgg agaagggagt
     4861 acccgtagca tatctctatg ctgaaacatc caatcatatt agcatttctg gcgaccatgg
     4921 gaacttgggg aatgaccgcg atcggagcgg cgttcgtctt cttcttcaag acgatcaacc
     4981 gccacgtcat gaacggcatg ctgggcttcg ccagcggcgt gatgattgca gcatcattct
     5041 ggtcgctgct ccagccggcg atcgaactcg ccgaaggagg atcccttcct ccgtgggcgg
     5101 tcgccgcgat cgggtttctc gccggcgggg cgttcctctg gctcagtgac caattgatcc
     5161 cccacgccca cctggactca aaaccgggtg aaagcgaagg aatccccacc aaccttcgcc
     5221 gctcgattct gttggttttg gcaatcaccc tgcacaatat ccccgaaggc ctcgccgtcg
     5281 gcgtggcgat cggagcagcg gcggcgggaa gcggatccca gacgatgact ggagcgttga
     5341 tcgtggcgat cggaatcggc ttgcagaacc tccccgaagg ggcggcggtt tccgtcccct
     5401 tgcgaagaga ggggctttcc cgcagaaaga gcttcttcta cggtcaggcc tcgggagtcg
     5461 tcgaaccgat cgccggagtc ctcggagcct ggctggtcac cacgatgacc ccgatcctcc
     5521 cctacgccct tgcgttcgcc gccggagcga tgatctacgt ggtcattgaa gagctgatcc
     5581 ccgagtcaca gcaagtctat ggaacggcgg atgaccactc ggccacccac tgggcgacga
     5641 tcggcacgat gctgggcttc accatcatga tgattctgga tgtcgccctc ggctagatcc
     5701 gctggagcag attttccacc gtcaaggtgc cgagcgagga gatccctttc tcggcaagct
     5761 gttttgcgac cgcctctccg gcggtatccc ggctcatctc atcgatcgtt tcgattccgt
     5821 agaggtgttc gacaagatcc tggagccgga tctgatcaag gggtttgttg ggaatgaagc
     5881 ttgtccggct gttgttcgtc gcgacgatgt acccttggtt tgtaagagcg ccgaggaacc
     5941 cctgaagccg atcgtatgga atgaccaatc ggtcgaggag ctccttgacc gtcgtggcac
     6001 ccttgccgtc ccggaagttc gcaccgatca ccatcatgat gttggtaccg tcggtcatct
     6061 gggtcgccgg gctctgcgga aggccttcac tgaccacccc ggttggtcga tattgatata
     6121 catatgccag ctcgacgctg aaaaagatcg tcgcccagaa cacataacag ataaacagaa
     6181 acatgaacaa ggctgcaaac gatccgtaga tcacgctgaa cgtcgccgcc cgactggtca
     6241 acaggccggt gaactgcgtg aagacggtga tgatcacgac gcccgagacg ctgccgatta
     6301 gcgcggcgct gaactggacc ttggtgttcg gaacgaagaa gatcagcagg aagaagatga
     6361 tgaagatgat caggacggga atcaccttct tgatgaccac cgaccaggcg ccgaccgaat
     6421 acccgatgag attcagatac caatccgtga acaccgattg gacgctcaca tagagggcga
     6481 gaagcagcgt cgtgatgatg acgaaggtca tgtatcccat gaagcgtttg aagggattgg
     6541 tcgactgggc ggagcgatag atctggttga tcaccgacca gaccttgttg atcagaagca
     6601 ccatcgtgaa caaaaaagag atcagtccga cgattcccag cgtggtggcg tttcgggtat
     6661 acgtggtgag ggccgtcatc acctgttcgc tggtctgccc gccggtgagc tcggtaagcg
     6721 cttcggagag gaagttcaaa agcggttcca gcactccaag accgctgaag aatgagaaca
     6781 ggaacgtcaa tgccggaacg atcgccagca acgtcgaata caccagtccc gaggcggagt
     6841 tcaatacatt gtctttcatc gattggcgaa atgcgacgcc taagaattca ccgaaccgct
     6901 gtaccgccga cttgaacttt cccatcttcc tagatccttt tcttcatcca ttgggcgatg
     6961 agtttgtagg tcttggctcg atcggtttca ttcagaacct cgtggcgtcc tccttcgaca
     7021 agcgtaaggg tcacatcttt gactccggct tttttataga aatcgtagac ggagcgaacc
     7081 cctttggagt aatcgccgac gggatcgtcg ctgccgctga tgatgtacaa aggaacatca
     7141 ccgggcagtt gcttcgccaa attgggatca ttcgcgattt caatgccggt caaaagatcc
     7201 ttgaagaagg agctggtgca cacatatccg cacagcggat catcctcata gcgcttcacc
     7261 tgctcgggat ctcggcttag ccaatcaaac ttggagacgg gattcttgat ccgcttgttg
     7321 taggatccga agctcatctt gtcgagcagc tcatcgggat gcttgggccc atattttttt
     7381 acattgcgcc cggcgatcat ctttccggcc tttcccaaaa gacccttgcc ttggctggtg
     7441 cccacgatga ccaccccgtc gaacacatcg gaatgctgga caagcagcgt tcgggcaaga
     7501 aacgagccca tcgaatgacc caagaggaaa agaggttgat cgggatattc attgttgatg
     7561 agttcactca gttcaaaggc gtcatcggcg acccgttgcc agccgtcctc ctcggcaaag
     7621 aaccccagcg tcttgccttc agcggttttt ccgtggccgc gatggtcggg agcccagacg
     7681 gcgaagcctt gcttggtgag tgatttggca aactcatcat atcgctttga gtgctccgcc
     7741 attccgtgga ggatcagcag caaagccttg acggcaccct ctttgggcag ccaccggtga
     7801 taggagatcg gggtctgatc actggatatc atcgtcttga gttggggcat ggcggagttc
     7861 tccctgttga gttaacatat ataattctca tatcatgtta gcacatcatt tgatgtggat
     7921 atacacccaa aaggtgtaaa taattcattg gagcgtcaag atgctcttga tcacagccga
     7981 cattcatggc tcgaccgaag cactgaaaga aatcgtcgat atcgcagcgt cccggaaggt
     8041 ggagcaggtg ctgatcgccg gcgacctctg tccccaggaa ggatcggtct atgcctccct
     8101 tctttcaaga ttacccggcc tcgtccttgt ccgaggcaac agtgacgcct cctaccagtt
     8161 cgctcaggct cgaatccatc tgccacccct tgtgcgcacc ttgagctatc aaggccttcc
     8221 gatcaccctc acccatggtc atttggacgt tccgtacacc gtcggaggga tcgtcatcag
     8281 cgggcacacc cacattcctc atctgaagaa agatgctgat ggaacccttt ggctcaaccc
     8341 cggctccccg agccggccgc gttcatcaag cggcgcgacc tacgccctga ttgatacaga
     8401 gggtgttgga atccatcgtt tgaaaaccca ttccccgttt ctttaccacc gcttcaagag
     8461 ctcgtgatac gctgcccgaa gaagggcaag cggaacgacc tggctcttgg gaaagttgct
     8521 tcgaccgtca agaagctgtt gttcggcgaa ggggggatcc tgccagccca atcgacggtt
     8581 tatttcccgt tccaagctcc acgcccgctt tcctgcgctt gcgaggtgct gggcggtaaa
     8641 ggttcttcct tcgctgatcg aagcgataag agcggacatc gcaaagcgcg aacgccttcc
     8701 tttgatgtgt ggataccatc gaccaaagaa gggcagatcg acatacggag acaggccgac
     8761 cgactcctga gcaccccaga gccgttgaag gtacagagcc cacgcagcgc ttcgcttcgc
     8821 tttgccgcgc cggtagtgtc cgcctcggat gagttctccg agaacgatcc gatgatcgcc
     8881 catgctggcg agcaatgctt gggtgggaag cgcccgatag tcaaacggtc cgaggggttg
     8941 gctgccgatg cagtagcacc cgttctttcc gccatactct gcaaccagtt gatcgagtcg
     9001 ctttccaagc tgatgaccgc tcccctttcc gtaactgatc gcatcgagca ctttgaggta
     9061 gttggcgacc aggcgacttt gaagatcggg aagaaaggaa agggtcccct ctttccgggc
     9121 gacccgcgcc catcccaaca gaaccccgac ggatgcggga tcaagtccgt tctccttgca
     9181 ccgtctggca agctgggcaa tccgcgaagg ttcaaagatt tcgagattcg cccccaacgc
     9241 aagcgcttcg atgagggaat aggtatccgc ccgtccgtcg aaacgttcat acagatggac
     9301 gaggcgcaga tcatagcgat agcggtgggt tccgatcgcg gcccatccga aggtgttggc
     9361 tttctccaga agccggacat ccccttcctc atgaacccgt tgggcgagag cggagaatcg
     9421 gaggctttgt tcaagccgtt ttttaaagat gccgagccga ccggggtcat acccctcccg
     9481 tcccgagaca tggggaacaa ggccgagcag tttgacattc ttcagcccca tcacgagccc
     9541 caaggcggag gaaaagggaa tgttctgatt gttgatgcag atggaacagt ggggaatctg
     9601 ccgttcggca gcgggtccga tgcagaggat atgctcgatc ttcgcccggt gcttcgtctc
     9661 tccggtggga agatcgtgga agctctcggc atcgaagaac tccacttgat catgatcaag
     9721 tgtaacgccg gtcaaccggc ggcaacgacc ggtgaacacc acggccgaat aaccgcagtt
     9781 cagcaaggcg gtcgaaaagg atgtttgaac cgtcgagacg gcaagcttgc cgcgctgcgg
     9841 atccttggtt acgagggtca tagatccgca gctctccggt tccagatccg ctgccaaggc
     9901 aggggctaaa acaagggggt tatgcgcttc gaaaaaactc ggctcaagct tgtcaaagga
     9961 aacaaaccgc tcccaaagcg aaagagcgag cagtctgccg ccaattaact gattcgattc
    10021 ttcttcggag agttcgatca ggtgcgatgt ttctttctcc agatcgatgt gaaggagtct
    10081 gcgtccgcga tacgcaaagg atcgattctt ccgtctcctc atcttcggca acttcaccat
    10141 gaacatcctc tctgcaggga gtataccaca ggtgaaagtt cttagacgag agcgattgga
    10201 gtggatagca tcataatgct gcaggggaag aaagggttgg caaacaagcc ggtatgaagg
    10261 cgggcaaaag agagccttga aatcaccgtt ggggaaccat gtaacctgca agttgcacct
    10321 caaatcgcat ctatacgaat actcgatcaa ataatcatca aagacctctt gacagatata
    10381 tcgaggttcg atatattata tatcgaagta cgatatatcg taacacaata catcgtactt
    10441 caagacagcg tgggaggagc tatgaatgaa tacataaaaa agatctatgt gccgatgacc
    10501 gagagcgcat ttttcatcct gctgtgcctg caaaaagaaa atcatggcta tggaatcgtc
    10561 aaaagggttg aagaattgac tgacggcgaa gtcattcttg cgcccggaac gatgtacgga
    10621 agtctctcga aaatggaaaa ggacggaatc atccggttcg ttcgaaacga agacaagcgc
    10681 aagatctata tcatcaccga tttggggctc aacgtcctga agacggaact caagcggatc
    10741 gagcgcttat atcacatctc ccgggaggta ccagagaatg acgaagaagg tgtttaagtt
    10801 tttttcgatc acccagcatg taaaggaaca ggaatatctg aggagcatgc ataagttagg
    10861 atggaagctg gtcaaaacca ccgggatagg catgtaccat tttgaagcat gtgaaccgga
    10921 agacgtgatc taccagctcg attacaatgc ggagggattg gccaacaaag acgagtatgt
    10981 ccggatgttc gaagattgcg gctgggaata tatccaggaa ttcgtcggat acagctattt
    11041 cagaaaaccc gcggcaaaag gcaccgcgaa ggatgaagag atcttctgtg atgacgaatc
    11101 gagggctcag atgatggaac gtgttttcaa ggggaggctg atcccattgc ttgtcatctt
    11161 tttcgtggtc ttcatacctc agttcttcat gaacgtgacg actcgcaaaa gccttggaat
    11221 tgccattacc cttggtgcaa tacttctgta tatgtcaatt tttgtgtcgt tcaccgtgca
    11281 atacgtgaaa ctgaggaaca gaatgaaaaa atgaatatcg tatgacagca tgccctgcaa
    11341 agcgaaaaga ccatccggtt ttcggactga acggtctttt ttaccgactt cattcaccat
    11401 gcaggttctt ataatagaag accacaatct gggaacggtc tctgagatat aacttgtcga
    11461 gcagattcga gatgtagttg cgaaccgtac cctcgctcag aaagagcgac tgggcgatct
    11521 ccttgttgtt catcccctcg gcaagcagtt tgatgatatc cagttcacgc tcggtgatat
    11581 cgaaatcttc gatggatttt ttctgtcggt tcgacaacct gatgtccttg atcacctcgg
    11641 aatccaagac catgttgccg gccatcaccg tttggatcga ttgaaacaag gaatcaatgt
    11701 tatcctttaa aatataacct ttaacaccga tcttgatcgc tttgttgatg tattcctcat
    11761 ccttgaacgt cgtcaaaaga atgattttgg cttctttgtc gtactccaac agtttgctcg
    11821 caatatcgat tccgttctcg tccttcaacc ggatgtcctg cagcacgaga tcgattctat
    11881 gattcttata gatgtcaaaa gcctctttac gattttttgc agtatgaacc tcttcaatcc
    11941 cttcggcttt taaaatcatt tccaatgctt ttaaaacaag catatcatca tccaccagca
    12001 atattctcat atgcccttct ccatcaatac atgaatgccg aaaccccggt ctgtcgtgat
    12061 attgaacttc caaccccgtt cttcacattt tttcctgatg gaatacaaac caagtccttc
    12121 ctgaacttcc ttatttgatg ttgaaccgtc atcttcaatc tttaacacca ggaatcggcc
    12181 gctctcaacc agatagactt tgaaatgccc gccgtcggaa tgtttgatat agttcgtgag
    12241 cgattctctg acaatcgcga agatatcgta tttgacgcca tagctcagat tcaaatcgac
    12301 gatgtacgta agattcgaat tctcgacggt ctccagcagc tccatgatct tgttttccag
    12361 atcgaacgag ctgtccctca gatcatacat gaccctgcgg atatcctgta acccgttctc
    12421 cagcgtttcc ttcaacacca ggagatgctc cttcgtatcc ggttctccgg tcgtcattga
    12481 taacgctttc atattcaaaa tggcggccga caaggtatgt cccgccgtat catggatctc
    12541 acgggagatc cgattgcgtt cctccatcaa ggtcagtgtt tcgtcccgct ctttttcaat
    12601 cttcatgagg tcgatcgatc tggacaggcg attcacatca tcaagaagat tgtatttttc
    12661 cttcctcagc ttcacaacct cagcctcttt acttgcaaaa agataatccc atagcagcgt
    12721 gaagccgacc agagtcagat acagaacatg gaaatgtcca agaaacggaa ggaacagcaa
    12781 cagggagacc ttgttgaact gggaaaaagc gcttggcgca agcagcggaa gcaaaaagat
    12841 gaaccgtgga aacagtgctg cggccagaat cccgaaaagg aaaaaacctg ttttcgcttt
    12901 ttgattcgaa acgaccttga tgagcaggga atagctgaaa cccagaagaa cgagaagcac
    12961 gagtgattcc gtcgggatat cgcctttctc gaactcatag atacttatga tcagcgcaac
    13021 gatgccgtga agggtaatgg tactcatata tcaccggtga cgtttgtcat ttcaaatggt
    13081 gagtggattc actaaccaaa tctgcagtct cattgtacca tgatttcagc gaggtgaaga
    13141 catggtaatc aaagtagaaa accttatcaa agagttcaag gaaatccgag cgttgaactc
    13201 attcaatctg gagttgaaag aaggagagat cctcggactg atcgggccga acggctcggg
    13261 aaaatccact ctgctgctca gcgtcctggg aatctactca tatgaaaacg gcaacatcag
    13321 cgtgttcggc caggagctca aaccgaattc atacgagatc aaacgaaaga tcggatatgt
    13381 tccccaggac ttagcgttgt tcgaagagct gaccgtctac gagaacatcg attatttctg
    13441 tggactatat gtccatgaca ggaaaaaaag aaaggagctc gtggaaaaag cgatcggctt
    13501 cgtcggcttg gagaaattca gaaaattcgt cccgtcaaaa ctctcagggg ggcttgcccg
    13561 ccggctcaac atcgcctgtg gaatcgccca tgagcccgag ctgatcttcc tggatgagcc
    13621 gaccgtggcc gtcgacgccc aaagcagaag cttcatcctg gaggggatca aggagctcaa
    13681 ccggcgaggg gccaccgtca tctatacatc gcattatctt gaagaggtcg aagacctgtg
    13741 tgaccggatc gccatcatcg accagggacg aaacatcatc acgggcaccg ctgaagagct
    13801 gagggattcg atcatcacga cggaaatcat caagatcacc ctcctggaag agcatgtgga
    13861 tatcttaaaa gatttgagaa acctctccca tgtcatcgac gttacgatca aggattcgga
    13921 atacaccgtc aaattcgaca acaacaaggg caatatcggc gatgtcgccc agttcctgaa
    13981 agagaaagga ttgacgtacg tgaagctgta tacggaagtg ccgacgttga acgacgtgtt
    14041 tctggaattg accggcaagg agctgagaga ctgatgattc gaatactcac ctatatcaaa
    14101 tatgaattga aagtgtttct gggaaactac ttcaacatgt tctggatgat gggctttccc
    14161 ctggtgctgc tgacgatctt caccattttc ttcgctcaac tgcgattcag ggagatcgag
    14221 gtggaaaaag tgaccatcgc ctatcatgaa aacgccttca ttccgctctc cttcgtcgac
    14281 gagccgaaaa agcggccgga ctttgacacg attgacagat cctccatcga gaccagactg
    14341 tttgaagggg cgatgctgga aaaggatcag gggcttgaag agctcagaaa caatcggatc
    14401 gatgcgtacg tcgacaagga cctgaacctc ttcatcaaca agaacgggat cggccagttg
    14461 atcatggatg aagtcctcac gacgaccaag cagatctcct cgttgtggct gccgtttgac
    14521 gcctatgatt tcgagcggac ctatgtgaaa agcagcgacg aaaagcaaag ccccatcgat
    14581 gtcatcttct atgcgctctt ttccatgatc agtctctacg gtgcgtatct ttcaagcacc
    14641 atcggcaatc ggctgattgc cggcagacag acggtcgcat tgagaaacgc ttcatcgccg
    14701 acatccaagg cttggcaagt catgatcagc gtcgtcacca gctatctctg gtcattgttc
    14761 ctggtcgcgg tgatagtggt ctatttggaa ttcatcttga aacaacattt cttttcaccc
    14821 gatctcaaca atatccccct cgtcctggcg gctctgacct tcggaatcgc gtggggactc
    14881 tttttaggga cggtcatcga aaaagaatcc ctccagacgg cggcaaccat cagctcgctg
    14941 ctgattttgt ccgggatcag cggactgtac agccacggca tccgcctgat catcaagcgc
    15001 tccttcccgc ttcttctcaa gctgaatccc gtttcactga tcagcgacga gttgctgaaa
    15061 gtgaatatgc tgggcaatta ctcgacgctc aaaggctcga ttcttctctt gacggggtac
    15121 agcatcgttc tcgtcatcgt ttccattctt cacataagga gaaagaaggc atgaacacgc
    15181 ttcagtactt catcaagata gccaacaaga ataaaatcct gatcatcgcc tatatcgcga
    15241 ttctcatcgc tttcacctcc ttatcaatga gttcctatga ggatgcgtat tcaaaaaggc
    15301 cgttgaacat aggaatcgtc aagcaggaag acagtcaatt cggaaattca ctgatcgagc
    15361 atctggggaa gggaaacacc ctctatcact atgattccca agagctggcg gaactggatt
    15421 tatacactcg cttcatcgac ggaatcgtgg tcgttccggt cgattcggaa agaatcctcc
    15481 tggaaacgga tgattcgcca ttgtcgatcc gcacggacgt caccaacagt caaagcatct
    15541 ttttacaaag agccgtagag acctacgctc tgtactacaa agcgatggtc gatgccggca
    15601 aactggatct gcacaagctt tcaaccgtgc tggatgagcg ggtggaggtc gtcttctcca
    15661 ccgatcgatc ccctctcgaa aagaaattcc atggattcgc gaatgtgtac gggtttgtca
    15721 tcatgatgat tctcttccgg ctcctgggcg acctgagcat cagtttcaac aagaaagaca
    15781 tccagatccg caacagaatc tcccccaaaa gcgatgcccg gctgaaagga gagatcagtc
    15841 tggcccagat gatcatcgct cttcttgtct tcgttcttgt aacggggttc atcctgaccg
    15901 tgttccttcc ggagatgttg cgatcccgaa gtttgggatt ctatctgctg atcctcttcc
    15961 tgtggacggc ggtcgtcgcg ttgctctcgg gcctgatcaa ccagctgtcc aagacacgat
    16021 cgctgaactt gacgatcggc aacatgcttc cgctcatcat catgttcctg tcgggatcta
    16081 tgcttcccat tcaattcatg ccggttttca tgcagaggat cgccagattc agtccgctgt
    16141 tctactacaa caaagggatc atgagcatat cggaaggcga tttcgacatc ggtggcgaat
    16201 tgctgatcat cgcggctttt ggactggcct tctacctttc ctccctgtat ctcagcaagg
    16261 aacggaaaac gagaacgctt taatcatgaa aacaatttct cgcggcatcc gaatactcct
    16321 catcgtcttt ctgctggcgc tgttcatcta caacaggctg atgtacataa aggaagaacc
    16381 gctgcgccgg ccgctcggac aactcgtcga agtggacggt cggaacatga gcgtctatac
    16441 cgagggcaag ggagaacgca cccttgtgtt catggcggct tcggcggtag cggcaccgat
    16501 tcttgagtac aagcctttgt acacgctact cggtgacgat tacaggatcg tcgtcattga
    16561 gaagttcggc tatggcttca gtgacgtggt cgatggtgaa agatcgttcg agacgatgct
    16621 caggcaggac cgggaagccc tttcaaaggc gggcattgaa ggaccgttca tcctctgtcc
    16681 ccactcgatg tcgggaatcg aagcgatcct ctgggcgcag atgtatccgg atgaagtcga
    16741 agcaatcata ggactcgata tgagcgtacc gggtgcgtat ggtaaggaag acttcagctg
    16801 gaaacggcat ctgctcgcat atcttctcgc tgccggacgt gaaatcggaa tagtcagatt
    16861 attttttacc gacagcacct tctcggatgc ccttacgcac catgaaaagg atatctaccg
    16921 agccgttgcc agctcaaatt actgcaataa gaacgtccgt tccgagggga aacatatccc
    16981 aaaagccatt gatctgataa acagcgaacc caagccggat ctccctatgc tcatgttcat
    17041 ctcggacggt tcggaaacag gaggagaaag ctggatcgag gcacagaaag agtatgcttc
    17101 cgatctgacg gatgctcaaa cgatcgacct tgactgcgga cacatgatac acaagtacag
    17161 acaagaacag ataaccaagg agatgagggc gttcattgag agcctgaacg gattggaaag
    17221 gtaaaaatga caacacatca aaccatcacc cacccgctca tgcctgtttt cggaccgatt
    17281 cgaagatcct gatcctgggt tcctttccct ccgtcaaaac cagagaatac ggcttcttct
    17341 atggacatcc acaaaatcgt ttctggccgt tgcttgaacg gatattcggt gtgaagccgc
    17401 ttgcgggcat tgaagaaaaa agagactttc tgctgcagca tcatcttgcc ctctacgatg
    17461 tcatttaccg gtgtgacacc attccgagat ggcctaccat cgattaagag aacatgcgag
    17521 cacgctgatc atcttttcac tcattcatat aatgaacgta cagcttttag tgaaaatcag
    17581 gctcgttcgg cctcctcatc gtaaagctat cgttataaaa tccggacttc cggggtaggg
    17641 cttatttggt ttgatgctct tctgcacaac ataagctcat accgatcaaa actacgcatg
    17701 aattaagaac cgatagagcg tgtgaatcag ctgttcccct cacaggatgt caacgactat
    17761 tcctgatgaa taaagcagtg accaaacaga tgagacagtc acttgactat accctatctg
    17821 atagggtatt ttatagacag cttatgctga tcaatcgtga atttgtcatg atgccggagt
    17881 ttgatcgcca atggaaacac cttgggcttg gtgatgagga acttcggcag ctacaggaaa
    17941 agttattgga gaatcccaag gcaggcgctg tgctgagagg cactggaggg cttcgtaaat
    18001 atcggatcgc ctttccgggt cgcggtaaga gcggtggtgg gcgggtggca tatgttgact
    18061 tcaccgtaca tgagacaatc tacttgatca cggcatatcc aaaatccgag aaggacaacc
    18121 taacggacaa ggaaaggaat gaaatcgcga aagtgatcgc cgttcttgaa aaaggtctgc
    18181 agaaacagga gataagtaaa tgagcgtata taaaagcatc atgcaagggc tggatgaagc
    18241 cgtcaaatac caagaaggaa agattgatgc acgaaagaca aaaattacag ttaaacaggt
    18301 ggagtctttt tccagtgagg acatcaagca aatccgaaag caagttggct tgagtcagat
    18361 cgtctttgca tcttcccttg gcgtgtccaa aaaaacagtc gaggcttggg agcgagggag
    18421 aaatactccg gcaggtccct cccgaagact ccttcagctg atcagagaca atcctgaagt
    18481 gattgaacaa tacatgatca aagcgtaagt ggcacatcag aatcattttc attctacaaa
    18541 ctgcgtctcc ttgaaggagc tcgacaatcc gagctgcatg caggatctgc tgtctggaac
    18601 atgtgccatc gggcgattct tgaaggattg catgattgtt catacacagc accttctcgg
    18661 ataagaacgt ccgttccgag ggaaaacaca tcccaaaagc catcgatctg ataaacagtg
    18721 aacccaagcc ggatctccct atgctcatgt tcatctcgga cggttcggaa acaggaggag
    18781 aaagctggat cgaggcacag aaagagtatg cttccgatct gacggatgct caaacgatcg
    18841 accttgactg cggacacatg atacacaagt acagacaaga acagataacc aaggagatga
    18901 gggcgttcat tgagagcctg aacggaattg cggattctat cccagcccga agccggcacg
    18961 gaactcggtg ggggtaacac caaactgctt cttgaacgcg cgggagaaac tccttggttc
    19021 cgtatagccg accaaggagc acacttcgta aatcttgata tccggcagct ggagatattc
    19081 acaggccttt gacatcctga cctgcgtcag atacgggaag atattcatcc cggtttttcg
    19141 tttgaatacc cggctgatat agaacacatt caggttcagc tcatcggcga tggagtcaag
    19201 gcaagccgtt gcataatgct cctcgatata gcgctttatt ctttccacga tgccgctttc
    19261 ctcgggaacg ttcatatcca gagccgcacg cgcctgctcg tccagctccc gtttcagccg
    19321 ggtgaagacc ctgaccaatt cttcatactt ggtcggtttc accacatagt ccttcacctg
    19381 gtaccgcaga ctctcctgtg catattcaaa ctcccgaaac gcactgagaa gaaccacttt
    19441 tatacccagc cggttctcat ggatgtattt cgagacctca atgccggtca ttttcggcat
    19501 gacgatgtcc aaaagaacga tatctacctc attgtcctcc aggaactcaa tcacttcctg
    19561 cccgttcgag cattgtccga caacctccat atcaaactgt gaccaaggga acaacaacgc
    19621 caaaccttct cgtattttcc gctcatcgtc cgcaatgaga agtttgtaca tagatcctcc
    19681 tttcagccac cgtctcctga attctaccct actttccgat ggtttggttg ctgatcttcc
    19741 gggtaaaatc ctccaacagg accacggtat ccgtagctct ggcatgctcg gcggcaaagg
    19801 cgatcaggtg gctctccagg gaaagggacg cctgagccct tgcctggtgg atatcattgt
    19861 tctgcagtct cgtcaccaca tcctgcatca ataggaaatc tcctccactg tggctgccga
    19921 catggattac cggaggtctg atgaccctct cttcatgtga accgaaagtg cgtacccgga
    19981 tgtagttttc ctccatattg gcaaagatat cccctttcgt ccccatgagc cggatctctc
    20041 tcgtctcaac cgtattgaac cccgaaagga cgaacgatac ggttatccga tcctcgaatt
    20101 cgagattcac cacctgatgg ttcgccaccg tattatctga gcggaaagcg catactccat
    20161 aattggtctc gcgcaatgcc tgcgtccgtg cctccagcga ggtgtccacg ctgatcatgc
    20221 tggtcggcca cccggtatcg gaaccgagat accgttttac agccgagaac gggcatgtcg
    20281 cttccaccgg gcagtcgagg caccgatagg tggaaccctc cggagcgtgc tcctcggtaa
    20341 agaaatccaa gtgcccgaac gaggaaacct tcttgcaacg ccttcccgtc agccagcaga
    20401 ggatatcgag atcatgacag gattttgcaa gaaggattgg gctgagattc cggaagttcc
    20461 ccctgacata ggagtgaacc agatggtcga cgctcacatt ctccgaatga tggatggcaa
    20521 ccagatcgcc gatggcacgt tcggcatgca caagctgatg aatggtgctg aagaccggtg
    20581 tatatcgaag cacatggcca aggatcaggg agaccccgtt ctgctccgcc aatcgaacca
    20641 gttcgacaca ctcccaggca tggggcgaca tcggcttctc caacaggcaa tgatgcttgc
    20701 gcttgagcac ttctttcgca attgcaaaat gggagtcgtc attggtgctt acgatgacca
    20761 gatcgacatc ctccaccgct tcaagcattt ccgatgcatc cgtgtacaac cggtccggtg
    20821 gcaaggaaaa catcgcgcca aacgcctcca accgatcctt tgaactgtcg caggcataga
    20881 agacacgggc agcatcggga tgtagaagcg agtatcctgc atacacggtt ccccgattcc
    20941 cgcagccgat gacggccagc ttgattgttt tcatttcgct ctccttttgc tcagcctttc
    21001 aggccggatg ttgcaatgcc ttgcacaaaa tagggttgtg ccaagaaaaa catgacgatg
    21061 atgggtatca ggcttatcaa cgagcacgca agaagcggcc cccaggcgac gctcgaggtg
    21121 atatccatca agcttctcag aaacaacgag acggtgaatt tgtcgaccga actgatgtag
    21181 atcaactggt tcaggtagtc atcccacgtc cacatgaacg caaagatgcc gacgctgaac
    21241 atcgccggct tggatagcgg cgcgatgatc cggaatgcga tgccgaagca gccgcagccg
    21301 tctatctttg ccgcggagtc caactctttc ggaatccctc tgatgaactg gaccagaaga
    21361 tagatgtgaa aggactgggt ggcgcagaaa gcgggaacga tgaacggcaa atagctgttt
    21421 atccacccaa aggcggtgaa cgtgagatat ttgggtatca acgtaacctg agcgggaagc
    21481 atcatggttg aaagcatgaa ggcgaacaga ggttttcttc ctttgaactg cagccgggca
    21541 aacgcatacg ctgtcacaaa gcttgatgct atcgtgccta tgaccaccgc acctaccatc
    21601 aggaacgtgt tcatcatgaa gtggccgaaa tagtacgtcg ggttgatatt ccagccatcg
    21661 atgaaatttt gcacggtcaa gcgcgacccg atcaaagaga attcccgttg aaatatctca
    21721 tttcccggtt tgaaggcgct gctgaccagc cagatcaacg ggaataccat gatgaagcat
    21781 ccgaaaatca gaaagacgtg ggtcaacagc ctcaaccatt gtcgcttggt tatttgagga
    21841 attctcagaa ccgttgcggg tgtgttcatg gcactgctcc tattcctgat agtagaccca
    21901 ccgcttggac gacaggaata ccaatccggt tgaagccaga ataatgaggg tcagcaccca
    21961 tgcaagagca ctggaatacc ccatccgatg ggagacgaat gccgtccgat acagatgcag
    22021 catgtagaca taggttgagt cggccggtcc tccgttggtg atcaccgcga tttgggtaaa
    22081 tacctgaaat gcgttgatca gccccatgac gaggttgaag agaatggttg aagaaatcat
    22141 cggcagcgta atggagaaga actgtcttgt cttctttgtg ccatccacct cggctgcttc
    22201 atacaactcc tgtggcacct gcttcaaacc ggcaaggaag atgaccatcg atgaaccgaa
    22261 ttgccaggca cccatcatcg ccaaggtttc caaggcgtac gatggactgg acaggaagtc
    22321 tatgggccca agatgcaggt agcccaatac cgcatttacg aacccttccc gtgaaagcac
    22381 catcttccat gtcaccgcca tcgccacact ggaaccgacg atggaaggaa ggtagtagat
    22441 cgtgcggtac agagatatcc ccttgatatt cctgttcaat acctgcgcaa ccagcagcgc
    22501 cgcaaccaat ttgatcggaa cgaacaacag aacatacttg aacgtgagaa acaccgatgt
    22561 cttgaacatc cgatcctcaa agaacatctc gcggaagttc tcaagaccga caaatgaggc
    22621 ctttccatag atccgatagt cggcgaatga gagaaacagc gattgcaaca tcggccagag
    22681 ggtaaagaga agcacaccgg ctatccaggg aaggaggaga gcatacgctc tccagccttc
    22741 ctcttcaaac actcgcctct ttacacgaaa agccttcatg gtgcgttcct tcagatatag
    22801 cgtgccagaa tcttgttgct ctcagcttcc aggatcgata acgtctcctg ggcactcttg
    22861 tacttcccga acaaatagtt ctggatttcc cgagtcatca aggtgaagat ttccataaat
    22921 cccgccggga acggttcgtt gacaaaatcc gtggaggtcg tcttctggat acaggcaagc
    22981 atggtttgtt cagtcgggga caacgaccgg ccgatattcg ccctgatgac gctgtttgaa
    23041 ggagtaccgc ggacaacttt caggatcgag cccgcctctt catcgttgac catgaagttc
    23101 agaagcgatg cggtcaattc ggggtccttg gcatccttgg tgatggagta catcatcgag
    23161 ggattaaccc agaagacatc cttctcaatt cctgcaggca cccgttcgat gccaaggccg
    23221 ggtacggcct cctgatacat catcgtgttg ggggaactgg caatgatcat cgccaccctt
    23281 ccgagttgga agtcgtccac accgtcggga ttggccgccg tcatctcaat cgggggagtg
    23341 atccccatgg tctggaaact cctgatgaac tccaaggccg caacaccggc ttccgaattg
    23401 aaggccacct ttgaaagatc atcagtccaa agcgatccgt tccgttcata caggtacttc
    23461 ttgaacgaca tgtcgactcc ggcgaccttg gccatctcgc cggttcccca gacatcaact
    23521 tttccatcgc cgttggtatc tctggtcagt ttctttgcgg cctcgatata ctcgttccat
    23581 gtccagctct cgttgggata tgctacaccg gcggcatcaa acatatcctt gttgtagaac
    23641 agcaacgtag tgcttacacc ggcgggtatg gcgtagagct tgccgtcaaa gcggtacatg
    23701 tcaagggtcg cttccgatag attgtccact gagatgatgc ctttttcaat aaagggatcc
    23761 agcggcagca gttggccgaa tgtcacgtag tcggcgacgt tggtgtagga aagctgaacc
    23821 aaatccggtc ccgtcttcgc gctcaattgc gtggtgagct tgtcgaaata tccggagaaa
    23881 ccgctgtact ccgcctgcag cttcacattc gggtttctcg actcaaacag cttcatggct
    23941 tccactgtcg ccgcatgtct ggcatcgctg ccccaccaca tgaagcgaac ttctttggtg
    24001 gcttcacggg ccgccacact cacactcgtg ctctccaccg gtttctttcc acagccgatc
    24061 aacatgccca gcattaccac cagcagaatg agaatgactc ctttcttttt catgtctact
    24121 cctttttcct atccgaatgg atacatgtat atacctttat agtaaagtca tgtatcggaa
    24181 aggagtgaga agcgatactg ctcaatttcg agtcatttct tgacgtattc ccatagttcc
    24241 gtaacaattt ttgcgcatcg gtcaagaatt gtgcccatcg tccattggaa tgaagggaat
    24301 caggatcgtg atgctcgtcc ctgctctcca ttcgctttct atggtgaact gcacactgtc
    24361 gccatagtga agcttcagcc gtttatacac gttcttcaaa ccgatatggg actcctccat
    24421 cccttcctct tccaatcgga gcctcaccat ggccaattgc tcaggcttca ttccggtgcc
    24481 ggtatcggag atgcgaaggg aaatgaaatc atccaaccgg cgcacctgaa tggtgatggt
    24541 gctcaacgcc tctttttcct tcgctccatg atagatcgca ttttctatca atggctgcag
    24601 caacagcttc atgcctttag aggagagggc ttcccgatcg atatcccaga gcacctcgat
    24661 cgtatcgctg taccgttgct gttggatggc gacatagcat tgggcatagt acagctcttg
    24721 tttcaacgta acggattcac tgggattgct tagcgagtat ttcaggacat cggaaagatt
    24781 ttgcaccatg ctgctgatgg tgttttgcaa cgagccggcg agccgcgacg cttcccagtt
    24841 gatggtttcc aaggtattga aaaggaaatg cgggttgatt tgcatttgca gcgccatcag
    24901 ttccagtgca ttgaacttgc ttttcttttc gatgagctgg attcttgtct cctcgcttgc
    24961 cacgaacaac ctgatcattt cttcaatcag gtaaccatac tcatcctctc ttcgcacaat
    25021 gcgccgggga atcgtttcgc ctttggcggc ggcatcgaga attccggaaa tcacttcaag
    25081 gcgtctggtg ttccatctga catcaagata ggcgatgacc agtaccagaa tggcgagcag
    25141 gaagatcacc aacaggagaa tttttgatag ttgaacgggc agcttgaaca gctgggcata
    25201 gggagtcatc gagacgctgg aaaacatgga acccttctcg accactttca aataatctcc
    25261 gttttcacgc atcgcacctt tggaatagga gtagatggtc tctccctgtt cattgtagat
    25321 ttcaatggtc tgtccttcca ccaagagcaa tcgacgcatc aaggagtcaa aatatgaagg
    25381 tttgatattg agaaccacca ctcctaccgg atgaattccg ctgcgaatga tgcgaaatac
    25441 ggtgatgatt tcctgattct cttcaaaact gaaaaaccgg gtggttcttc tgaccgtcag
    25501 agcttccgga ttgcgtttgt tctgcaagct catcgccgta tcgatccaat tggtgtcata
    25561 gtatgagtga atgtcctgaa cgccgaggtt gcttaccaga aaacgatcgg aggtctcaaa
    25621 aaatgccaca tagcctgaat ggatgtaggg gcgggagttg gtatggttct ggaggtaata
    25681 gaggaattcc tgctcaacgg ccttccgttc ggaggagtac ccttcttcct gaagaagttc
    25741 caattgcccc gacttgatca gtgaatgatt tccgaaattc aggctcagca catggatctc
    25801 attgaagatg gcgcttgcat ggtcgagaat gtgccgggca acgttcatat tgttgttttg
    25861 tatctcggta aggaggaaac ttgatgagat gatgatgatc acaatcccca aaacgagcat
    25921 gaccgccagg attggaacgg caagcttaag atagttcgtc aggagtttgt gtttgttctc
    25981 gatggcgttt agcataggta gtgtaccatt atggcatggt ttaaggaatt tgcataacat
    26041 gacgtgcaat accttctcca atacgacgac ggaacgttgt catcgacttt ttaaatgtaa
    26101 gagaggcaac aatggccaac catcaaacca tcacccatcc gcttatgcct gtttttcaag
    26161 ccgattcgaa gatcctgatc ctgggttcct ttccctccgt aaaaaccaga gaatacggct
    26221 tcttctatgg acatccccaa aatcgtttct ggccgttgct tgagcggata ttcggtgtga
    26281 agccgcttgc gggcattgca gcaaaaagag cctttctgct gcagcatcat cttgccctct
    26341 acgatgtcat ttaccggtgt gacatcatcg gatccggtga tgccagcatc caaaacgtga
    26401 ttccctcgga tctaagcccc attttaaaag aggcggatat ccggcacatc ttctgcaacg
    26461 gcggaaccgc ttaccgctct tatcagaaat atcaggagcg gaaaacggga tgtaaagcca
    26521 ttcaactacc ctctaccagt ccggccaatg cacgatattc catggatgat ctgtatgatc
    26581 attggaagat actgagggaa tgttgagctg aaaggaatga cgtggttctt gatgcatctt
    26641 gcggggaata tgccatactg aacagacagg ccggcggaac ggttcggaat agaggtggaa
    26701 acatacaaga aggattgaag gtggatgata tggatttcag aaacaaggtg gcggttgtta
    26761 caggcggggc gaaaggcatc ggcaaatgca tttgcgacga gttcgagaag gccggtgcaa
    26821 cggtatgcgt catcgacgtg caggacaacc ggtattttaa aggagacctt gccgacaagc
    26881 ccacgctgga gcgttttgcg gacaaggtaa tcagcgagta cggccgcgtc gattatctga
    26941 taaacaatgc ggcgccgctt tccacaggaa tcgattccgc cacgtatgag gattttgaat
    27001 atgctttgaa agtgggggtg ctcgctccct tttatcttgc gaagctgttc aagccgcatt
    27061 ttgcggacag cgcaagcatc atcaacatct cttcatcccg ggaccggatg agccagatgc
    27121 aaaccgaaag ctatacggcg gccaagggtg gaatagccgc cctgacccac gccttggcga
    27181 tcagcttggc gggaaaggtc agagtgaatt cgatctctcc cggctggatc gacaccacag
    27241 atgcgataca tgaaggcccg gatgccgtcc agcatccggc aggcagagtc gggaacccca
    27301 tggatatcgc gaatacggtg ctctttctct gctccgacaa agccggattc atcacaggag
    27361 aaaacatctg tatcgacggc gggatgacca aacagatgat ttaccacggc gatcatgggt
    27421 ggacgctggg ctggagaaag ccatgcatgg gaatgcgtaa gcagcttgaa catgagagag
    27481 tatattgagg ttatcttgca agcattggca ggcgaactaa aaaatcccgt cgacacgagg
    27541 ccggcgggac agtaaaagaa tgtatagatc gtacaactta cattttacat tattgaaaaa
    27601 ttgaggaaaa tcgtcgccat caatgatgct accagcgata cgacggtgat ctgagtgttg
    27661 atcgaagggc cggcggtgtc cttgaaagga tcgcccacgg tgtcgccgat aacggcagcc
    27721 ttgtgtgctt cggatccctt gccgccttca ttgccggatt cgatatactt cttggcatta
    27781 tcccataacc ctccggcatt ggacatgaac aatgccagca acagccccgt gacgatgttg
    27841 ccgagcagga agcctccgac agcggacacg cctcccacga gacccaccag caccgtggcg
    27901 atgatggcca tcaatccggc gggaattagt tccttcaagg ctccggtggt tgctatgtca
    27961 atgcacctgt tgtaatcagg ttccacaccg ggggcgccat ctcggaggcc tttgatctca
    28021 cggaactggc gatgaatttc accgatcatg cgttgcgcgt ttttgtcaac acccagaatc
    28081 agcatggcgg agaatacggc cgggatggcg gcgcccacca aaatgccgaa gaagacggta
    28141 ggatctatga gattgaatcc ggtcaagacc tcccctgcac gccccatttg agccaatgcc
    28201 tcgttgactt cactcatgaa ggcacccagc agggcaatga ccgtcagtcc ggcggcaccg
    28261 atggcaaacc ctttcgtgac ggctttgacg gtgtttcccg cgctatccaa ttcgtccgcg
    28321 gcgcgaatgg cttcttcacc caggccgccc atttccgcca gaccgcgggc gttgtcaacg
    28381 atggggccat acgcatcgtt ggatatgatc attcctacga tggacagcat tcccaccgcc
    28441 gccatggaaa tgccatacat ggcatacccc gaaccaagtg gctcgcaaag cgtgtacgca
    28501 atcagcgctg acagcgcaat accgaccatg gccggcaaca cggacagaaa cccgtaggat
    28561 acaccggaaa gaatcgtaaa tgcgggacct gtttttgaag catgggccac tttttttacg
    28621 atgggccggt tgtcgtcagt gaagtaattg gtcgccagac cgataatcac gccgaccagc
    28681 aggcctgccg tggatgcgcc ccatacgcgc cattgaaaac cggggataat gaacgtagcc
    28741 aatgccgtaa gaaccagaaa gacccccgtg gttacgtacg tggatgagtt cagcgctttt
    28801 gtaggactgc catgatcgcc aaccttggcc gtgaaaatac ccacgattga ggcgagaaga
    28861 ccaagagccg catagcagaa caccatcatc acgtttgcac cctgaccgcc atccagcatg
    28921 gtagccatga caagggcggc ggacattgac gcaacgttgg aatcgaacag gtcggcaccc
    28981 atgccggcca catcgcccac attgtcccca acgttatcgg cgataacggc cgggttgcgc
    29041 ggatcatctt ccgggatgtt gagctcaact ttgcctacca ggtcggcgct gacatccgcg
    29101 gttttggtga atatgccgcc gcctgccttg gcaaacagcg ccaaggagct ggctccgaac
    29161 gaaaagctca gcagcaccgt gggattgcct atcagcagca tgacacccat cacgccaagc
    29221 agacatgctc ccaccaccag aagccccatg accgcaccgc cgcggaaagc aatgcgaaat
    29281 gccggtttga ttccatgcct ggcggcctcg gcggtacgtg cattggcgag cgaggccaca
    29341 tagattccga cttttccggc aatggccgaa aacaccgtgc cgaacagata cacgatcgcc
    29401 atgacggcat tcatgccgac agtgccggac gtccagacgg gcttgggcaa aaagatgaat
    29461 atcaggacgg cgacaatcga ggaaaatagg gcaagaatgc gatattcttt tttcataaaa
    29521 gcatttgcgc cgcttcgaat aagaccggca atacgctcga tggtcgtatt ttcgtttttc
    29581 tgcgccttga cccatctgct cagatacaat gcatacaaaa atgcggcgac gatggtcacc
    29641 gcgctcaatc catagatggc cgccatgata ttgggttcca tgccaatttc tcctatgatc
    29701 ataatagttc tgaaaacaat gaatttaatc aagcgagatt ttacatctcc atacgaaatt
    29761 atttgaatcc atcaaaaacc aatgcgccaa gcaaccgctc catgccgcaa aagttaattg
    29821 ggagggtcgt tataagttcc accgttagaa ttcttttcag aagtcggcca tcccaacccg
    29881 gacctcttgt tcgatgtgat gtgaatcact atcattggca tatgagaaac attaccgtat
    29941 cgattggttc tgaacattat aaagtagcga tcgggacgac ggtcgaagag gttctggtca
    30001 tcgcgaatct ggtcaccgat tgcaaagccc aaacgtataa ggacaatcct tacatcggcg
    30061 cattgctgaa cggcgagctt cactcctgta cccgcagtct ccttgccgat tgtacgttgg
    30121 agccggtcca tctcttcagc gacatgggca agcgggtcta tcgccactcg atctcttact
    30181 tgctctgtgc cgccgtatcg atgctctacc ctcatcgaag attgatcatc ggccacgctt
    30241 tgggcgacgg ctactacttc cgttttgatg ataaactgac cttgggtatc gatgatattt
    30301 ccaccattga aaagacgatg cgaatgctcg ttaaacagga tctcccgatt gaagaggtca
    30361 cgatgaccta tcgggccgct cttgcgtatt ttgaacagaa caacttcaat cacaccgtct
    30421 ctctcctgag cacccgcaat gatcccaaga tcaccctcta ccgtcttcaa ggctacttgg
    30481 acatctccta cgagccgctt ctttcccgaa cctgccagat ggaggtgtgg gaactcatcc
    30541 cgtatcagga tcgagggatg ctccttcgct atccccgcac ccacaacgtc atgggtctgg
    30601 atcctttcgt ggacaacccc aagctctttt ccgtcttcag ggagtacaag cggtggggtt
    30661 ccattctgaa cgtgcaatcc cttggccagt tgaacaaact gggcaacgct cataagatcg
    30721 aatccttcat ccgcctggcc gaggctcttc agcagaagaa gatcgtgaac accgccgaca
    30781 tgatcgccga gcgcagcggc gtcaaagcga tacttatcgc cggtccctcc tcctccggga
    30841 aaacgacctt tgcccacaag ctggcgatcc agctccaagt cttgggaaag aaaacgattc
    30901 agatctcgat ggacaactac tatctcagtc ccgaccgggt tccgaaggat gagtttggga
    30961 agccggactt ggaagcgttt gaagcgttgg acatcgcaag cttccaagag gatttatatg
    31021 ccctcatgaa tggtgaggag gtgcatctgc cccggtacga tttcaagacc cagatccgga
    31081 gctttgaaaa ggagccgtgc cggttggaca atcggacggt gctcatcatc gaagggatcc
    31141 acgggatgaa tccgggtctg accaactcca tcgagagcga aagcctgttt aaaatctata
    31201 tctccgccct cacccagatc aacctcgatg accacaaccg gatttcgacg acggacaacc
    31261 ggatcctcag gcgcatcgtc cgcgaccacc gaactcgcgg aacggatgcg gagacgacct
    31321 tgaacatgtg gccttcggtc caccggggag aagaccggta tatcttcccc tatcaatcga
    31381 acgccgatgt gatgatcaac agtgcccttg attatgaact cggtgttctt gttacgtacg
    31441 cccaaccgct cttgcgaatg gtgaaaccaa gcgccggccc ggcatatgaa accgcccgac
    31501 ggcttctgcg cttcctcgag cacgcaaatc caatcccgga tacactggtt ccctccgact
    31561 cgcttcttcg cgagtttatc gggggcagtg aattcggagt catataggag tattgtatgg
    31621 aagtttatgg tgtcaagaag atccgaaaat cggatatcga acgcgctttg ggtacagcgg
    31681 tcaccctcta taaggaatcg gttaccagct tgggtgagtg cacccttgcc ttggtgagaa
    31741 acggcaagaa gaagtatctg atcgcaaagg gttccggtcc gatgtttgat gaactggaag
    31801 gcgaggtgac ggacgacctc aagatctgtc ccgccaatca cgccaaccgc ttggtcctga
    31861 acacctatct gccctacacc aaacccacca ccaacaagga cggccgcccc tcgatcggtc
    31921 tgggcgatcg cttgggtgaa gcaacccccg ggcatatcaa agcgctggga aacaagaaca
    31981 ttttccccta cttcgctcag cagtcgatca gagagctcaa cctgaccggc agaacctttg
    32041 acggtgtgat cgacgatgcc gcctatgcgg tattccagtg cgggtacacc gccggttggg
    32101 gtgccgacgg cgaccacctg aagaaggagg aggagatcaa gaccgccctt cggtgcggag
    32161 cgacgatgat caccctcgac tcttcggaga tgatcgataa taccatcgcc ggccttcctg
    32221 aaaaagaact gcttgtccga tacgggaacg tcgatgaaaa aacgcggacg ttctatgaaa
    32281 acctctacaa ggagcgcacc ttcaccttcg gaacactcag cctcaccctc gataccgttt
    32341 cgttgatgaa ggatatcctc atctacggaa aagcgttgga ttatattcaa aagatctggg
    32401 aaaccttccc cgaattcaag gcggatgaag cgttcctgga agtatcgatc gacgagaccg
    32461 ctacccccac cgacccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatcg
    32581 gcctttccac gtaaaaaccg ccggaacgaa ctggttggaa gcgatgcatg tggttgcatt
    32641 gaccgatcct tccctctatc gacggatgca tacgcacgcc cttgcccgct tcaaggatgc
    32701 aacggcgttc tacgtggtga cgaccgattt gagcaagatc aagccgctgg acaaggtatc
    32761 tgatcagcaa ctctgtgatt atctgaagga tgataatgcc cgacagctgc tccacatcac
    32821 ctatggatat ctgcttcagg ataaggatga gaaaggcgca tatctcttcc gtgatgagtt
    32881 ctttgctctt ctgggcaaag aagaggagct ctaccaagat cttcttgcca aacacatcgg
    32941 caagcacttt gagcttctgg ggtggaagaa gtaatcattt gagagaattg aggaagaagg
    33001 cggccggtcg gccgccttcc tcttttcccc gtcttccgac gatcacacgt tgtagatgtg
    33061 cttcagttca tcgaagagac cggcgatgtc cgccttgcac ttgccacagg tggttccgta
    33121 tttggtgatt tcctgaagct cttcgagcgt ggtcgcctgt ttcgtcttca ccagattctc
    33181 gatctgaaca tcggtgatgt tgtggcactc gcacacgatc ttcgcgaccg aatgccggta
    33241 cgggttgggc aagccgttct ttttcaggta atcatcgatc gccgctcgaa gccccttgtc
    33301 accgagaacc gaacagtgga acttgtgctc gggcagacct ccaagggctt cggtgatcat
    33361 ttcaggtgaa agatggtagg catcctcaag tgtaagaccg atcgccatct cactcagcat
    33421 cgaagtcgat gcaatcgccg aggcacaccc gtaggtaagc caacgaatcg ctttgatccg
    33481 atcgttttcc acctgaatca tgacgcgcat ctgatcacca caggcaagcg aaccgacgtc
    33541 gccgactcca tcaggaacaa agttctcctt ctcatcccac gtgtttcggg gattcatgaa
    33601 gtgatctttt acagcgggac tatataccca ttgagccata aagttactca taacgcagac
    33661 atctccctga gtcgcttgat aatcggaggc agcttctcca aaacataggc aacatcatct
    33721 tcggtgttgt accgaccgaa gctgaaacga atcgatccat gggccagctc gatgtcgacc
    33781 ccgctggaaa gcaatacata cgagggctcc agcgaaccgg aggcacaggc gctcccggtg
    33841 gaaacttgaa ttccttccaa atcgaggtag agtaaaatac tctctccttc ggcgtggggg
    33901 aaagacacat ccaacgttcc cggcaggcag tgctcctgat tcccgttgat cacgacattc
    33961 ggaatacgtt cgataatccc ctttcgaagc atctcgcgca tcttccaaag ccgctgctct
    34021 tcatctttcc gatcccgctt ggcgatcccg gctgcgacgc ccattccgat gatcgccacc
    34081 gtgttgtacg tcccggctct tcggcctgat tcctggtgtc cgccatggat gtacggggtg
    34141 aagggtgcgc cctttgcaac cgcaagcgct ccgactccct tcggaccgta gaacttatgg
    34201 gcggacaggg acaggtaatc cagtccaagc tccttcatcg acacctcaat ctttccgatc
    34261 gcttgcgtcg catcgctgtg cacataggct ccaaccttat gggccatttc aacaatctct
    34321 ttgatcggtt gaatcgtgcc gatttcattg tttccgaaca tcaccgaaac caaagcgaca
    34381 tcctcggcaa ggagttcctc caacacatca aggcggactc gaccggtgtt gtccaccgga
    34441 cagaggtcaa tcctgtatcc tcgacctcgg agataggcta ccgtctcgat gatcgaagga
    34501 tgttcaatct cggtggtgat gatccggttt cgcttcgacc cgaggtcgat ccgttcccgg
    34561 aaaatattga ataccgtatt gtttgcctcg ctcgctccgc tggtgaagac gatcgaaccg
    34621 cttggtgcgc cgattacgtc ggcgactgcc gatcgggctt cctcgatctt gaaggcggcg
    34681 tcgcgcccga aggcatgcat gctggatgcg ttgccataga gcgcaagcgc ttcattcatc
    34741 gaagcgatca catcctcatg gagcggagtc gtggcgttgt tgtctaaata tacaaatctc
    34801 atgatacccc tcttactcaa cgttcatacc catgaccggg tttggatcaa ccgaaagctg
    34861 agtaaaaagg gtcctcccca tcctcggcgg gacaggtttt tgtatgaata atgcgcaaat
    34921 accgctttcg ggcgaaacgg atccgacccg ttcgcgccca ctgggcatac gcgcaggttt
    34981 caggaatata cgaggcgaac atcgcccgcc ataccgtcat cttcagacag cgcttgcttt
    35041 cgacgaagcg ctccgcatag acggtgcatt gcttgctctt tggatcgtag aactcgcacc
    35101 gggaatcaag atcgatgacc aacgtatttc cgctgacgac cttttcatgg cagcacagcc
    35161 cgcaccgtga gcatttgctc tcccacttcg cacccatcat ccacctccca aggaggcaag
    35221 cttcgtctgg actgcggaat tgattgaggg aaggacatca agggcggtta tgctgatata
    35281 tccgtcctga accgcttgga agtcgctttc ccgggtggcg catcgttgtg tgggaccggt
    35341 ccctcctttg agggtaagga tcaccgcggt tccgaagcga accgaggaag gatctacaac
    35401 atggacctcc tcgctcttct tcgtcgcaat cgcatcatgg tattcaaggt acgcaagatt
    35461 cgccggtttc gcctttccgt tcggacgggg aggcacattg atgctgatga tcgtcgtctc
    35521 gtcgacgaga tccatgaaaa atgcaaggtt gtcccgaacg aaccgagccc cttcgatgaa
    35581 cgcgaacctg ccgtcctcat ccttcgccgc gctgatcgcg atcgccttca tcccgttgat
    35641 cgccgcttca cgggcggcag cgaccgtacc ggagtagaga acatcggtgg agatgttgta
    35701 gccatggttg attccactga tcaccacatc gggtttttcg ccaaagagac cgacgcggga
    35761 ggcgtagaga atacagtcgg caggctttcc gctgcagtgg tagcggttcg gttgatattc
    35821 ggtaatcgtg atatcctcgc gaagagtcat cgcatggctg gacgcgcttc gttcaaaggt
    35881 gggagcacag atccaaatat cgtgaccggc tttttcaaga acatccgcga gggtattcag
    35941 tccctcgctt gtatatccat cgtcatttgt caagagtatc ttcatcaagc gtcaatcgtc
    36001 cgggaacaca accctcgccg gatcattcgc cccatagata tgccaccgag gcttgaatcc
    36061 gaagatgtgt tcatattcct ccagacggtc aatatagctt ggaagagcct ccttgctcaa
    36121 gaggaccatg atgttgccgg caaaaccgtt atccacctgc accgcaccca aacagccatc
    36181 aagttcaccc gctcgcttga tcagccaatc gatctcggga acggttactt ccaaaagatc
    36241 ccgcaacccc gtttgaatcc ggccgaagat ttttccgagc tgaggagcat cacgcggggt
    36301 caggaggttc gcggcatcct tgaccaactg cgattccatc agcacgtatt gggcgttgtg
    36361 ccgcgcatcc tcatcaaggt ggacaacccg cgaagtgatg tcgctttcgg ggaaatcacg
    36421 aatgaatgcc ccgctgtagt gttcgctcag ctgctcaaac gctgcttcaa tatcaagttt
    36481 tttatagtag atctcctccc gcattgcgct gggagggatc ttggagtcga cgatgagggc
    36541 cacgtactga gggctttctt cggtaaaggg aaatacccgt tcctgatacg tgacgtgctg
    36601 gaggtcaaag aaaaagagct tgtcccgtcc ggagttgagg attgtcagca aatcgctgac
    36661 tcggcatggt tcactgttga aggtcgtatt cgcttgatag accagacgaa tcgtcgcgct
    36721 gaagctcaag tcaagcttca agaggtggtc gacagcaaga acggttccga tcgcgctgga
    36781 agttgaaacc atttgattgt ccgcatagag caaccgccct ttgagggtga tgtcaagacc
    36841 gggaatttca tgattgtcgg cagacatcat cgcgatgacg cctttagggt agttgcccca
    36901 ccggtcttct ttacggaact tgatattgtt caagggaaat cgctttcgat cattcaaggt
    36961 cgcattgaac agcctaacca tgttgtcatc ccgtacggag atggcaacgt acaaggtcga
    37021 acggtcggtg ccgaccaaag accagccgcg gcatgcatcg gcgtatgcac ccagaactgt
    37081 acaagcacca ggcacctgca caatcacttc gggggtatga ccatactcct ctttatgctt
    37141 tgctatgagc tgttccatct ttcaccctta aattcaaggt cagtttagga tacttgaggg
    37201 gacaatgcaa caataaaacc aaaattgtag gatttggttt ctcctaaaaa tactgcttct
    37261 gtgcggtttt aaaggaaata tcagggatat acaggaatga aaaaaccacc cgaaggtggc
    37321 cttttcagta tcattacttt tccagactat agcgcttttt gaagcgttca atcctaccgg
    37381 cggtgtcgac cactttctgg gttcccgtga agaacgggtg gcacaccgag cagatttcga
    37441 cctccattga gcgggccgta gtcttggttt tgatcacgtt tccgcaggag cacttgatct
    37501 cggtgagctc atagcgcgga tgaatctttt ctttcatgct ttcctcctgg tatctcttcc
    37561 gctggcagta tacagcggac taccgttgtg tatcccttgc ggttaatatg agcttccgct
    37621 ggcggaaaca cctgtgttta ttgagcgcaa gaacgcctca ttatctttgg tcttgcggat
    37681 tttgtcaatg aggaacggtg ttatctcctg ttcatccata tcattgaccg cattgcgggt
    37741 cagccagatc cgagcggcct catcggaggg caaaagcagg tcttcccgcc tcgttccgct
    37801 cttcttgata ttgatcgccg ggaagaggcg tttatccgcc agacggcgat cgagaatgat
    37861 ttcgttatta ccggttccct tgaactcttc aaagattacc tcgtccatcc gcgatcccgt
    37921 ttcgatcaaa ccggttgcca caatcgtcaa cgatccgccg ctttcgatgt ttcgtgcggc
    37981 tccgaagaaa cgcttgggtt tgtgaagcgc gttggaatcg acacctccgg agaggatctt
    38041 gccgcttgcc ggaacggtct ggttgtacgc ccgggccaac cgggtgatcg agtcaagcag
    38101 gatgacgaca tccttcttgt gctcaacgag ccgtttcgcc ttttcgatca ccatttcggc
    38161 gacttgaacg tgcctgctcg cctgctcgtc gaacgtcgag gcgatcacct cgccttttac
    38221 gtgccggcgc atatcggtga cctcttccgg ccgttcatcc accaacagga ccatgagcgt
    38281 cacttccggg tggttgatcg cgatcgaatt ggcgatcttc tgaaggagaa ccgtttttcc
    38341 ggtccgcgga ggcgctacga tcagcgaccg ctggcctttg ccgatcggac agaacatatc
    38401 gataatccgg gttgaaatat cttcgtccac cgtttccaag cgcaagcgct catcgggaaa
    38461 gagcggagtc agattgtcga aaggaactcg gatcttggct tgaatcggat cgtctccgtt
    38521 aaccttcaag actttgagaa gggcgaaaaa gcgctcgttc tccttcgggg tccgcacctg
    38581 gccatagacc acgtcgccgg ttttcagata caggcttttg atctgggcgg gcgagacgta
    38641 gacatcttcc tgtcccgaga ggtaggagtt tgccggcgag cgaagaaacc cgaatccgtc
    38701 gggaaggatc tcaagcgttc ccgtgcccac caccaccccg ccttcgccgg tgtgccggcg
    38761 gagaatctcg gccacgatct cctgtttgcg cagatcgaga atcgcttctt catcgattcc
    38821 ccgttccact cctacttttc gcaatgtatc gattccgagc gtcgcataat actcaagctc
    38881 aagaaccgga gcatcggggt gttcctcgat attcagatcg atcgatgtat cttcgatatt
    38941 caaatgagcc gagggacggc cgggaccgaa gctttggctc ttttggtagg atccttttcg
    39001 attttgggtg tttcgacttg actggcgccc gcttgctttt cgttggggag cacgaggctt
    39061 ttcaacaggt gctgcgggtt tttcagcggg tgcgggaacc ggagtcgccg gttcttccac
    39121 aggagcggca gcttccggcg ggagcggatc ggcctttttc cttgggcgac cgacttttct
    39181 tttcacctct tccttcgggg cttcttcact gctctttttg acgacgactc gttttgcgac
    39241 ctttaccggc tttacttctt ccgccacctc ggctgaggtt ttttctactt cttcaactga
    39301 catgggaatc actccaacgg aaacatgcac ataatttggg cgcttttcgg gcgcaccacg
    39361 ccatttcctc taacggttac taagcatgta agcatatacg gggactatcg atacaatgca
    39421 ttaggacggt cccgtcatcc gtactcctaa ctatattgcc acttcttgac agtgtcaagc
    39481 aaacgcccgc cttggatgta gagcgtaccg aggagaaacg ccacggtggc ggcacggcga
    39541 cgcatgatat cccggctata tgcactgagg tgcatttgaa cgctttgtgt ctcccgtgtt
    39601 ctcgaagcga agccgaaaca cactgttccc acgggttttt cttcgcttcc gccgccgggg
    39661 ccggcgattc cggtcaccga aagcgcccaa tccaccgagc tttgattgag cattccttcc
    39721 gccatctcag cagcacattc gacgctcacc gcaccgaatg tggaaagcgt ttcctctttg
    39781 acgccgagga ggcgggtctt agcctcattt gcatatacgt tcaccccgcc ccaataccaa
    39841 gcggacgaac cgctttggtc ggtgaggagc tttgagacca gaccggcggt tatgctttca
    39901 gcggtagcga tcgattgccc ccgctccttc agctccctga tcagcagttc gaccggctgg
    39961 acatccccgt cgacaatgag gagcggtccg acaagcgacc ggagcttttc caccatcgcc
    40021 gtacgttttt cccgattccc cccggcgata tacaggctga tcgtctgttc ctgaaagcgg
    40081 gttccccatt caagtccctc gacggcgacg gatcggcaca gctcttccag ctttgattcg
    40141 gcaaggaggt aggttgagta atcactccgt tcaaaatcat catgaccgga gagctcgccg
    40201 agcaggggac gcacatgatt gaagaacatc ggatccatct cccggggggg accgggcagc
    40261 gcaacgacaa gaacctcccg ttcatcaact cggacaaatc ctcgaaaacc gcaggcggtt
    40321 ccgttcggat tgggaattac ctcaaagccc tccggaatca tcgtctgttg ttcgttcgcc
    40381 ccccagatcc gctcccccat ccgctgatag agagcttcaa acgccaacag atcacgaacc
    40441 agctccaccc cggccagatc ggcgacgatg cttcgggtcc ggtcgtcgct ggtggggccc
    40501 agcccgcccg tcacaaggac gacatcgcat gtcttcacac attctctgag gtgattggcg
    40561 accgttccgt catccgggag gataatgatc cgttcgaccc ggtagccgag ctgggtcaat
    40621 tgactgctga cgagtcttcc gtggcgatcg ctgatcactc cgcgggtgag ctcggtaccg
    40681 atgataaaga gtgctgcccg ggtgtagttc atcgtcgttt tcgtattgtc gtcaccacgc
    40741 agcggatcag tccgaagccc aacagggcat aggagagata gattgcaaca aaggcgaaga
    40801 gatcgatatg tcgggtatag aacgtgaagc catggctctc ttgcgtgtac acgggaacat
    40861 catagatctt ccaccccact ttgaagggtt ccatcatatc aagaatcttt ccattcacat
    40921 cgatgacgca cgtcattccg ctgttggtcc ctcgaaccgt cgtccgccgg ttttcgattg
    40981 atcggaagac cgccagaccg agatgctgca tctgggcact taccgctttc gaccagccgt
    41041 cgttcgtcat gttgacgatc acatccgccc cgtttctgac aaagtttgca ttgagatacc
    41101 cgaagacatc ttcgaaacag atcggagtgg agaagcgaac tccttcatcg gtcgtgaaca
    41161 ccaccgactc atagcctgtc tcccaccagt tgtaatcgtt cgccaacagg agccggtaca
    41221 gccacggcag ctgcttttca taggggaagt gctcggtgaa gggcacgagg tgctgcttgc
    41281 ggtaggtgtc tttgatcttg ccgttttcaa agaagatgac ggtattatag tccatccggt
    41341 tccactcgcc gtccgcaccg aatgccggaa gatcgggatt cttgatgacc ccttccgggt
    41401 ttcccgttac cagcggaacg ggaaggcttt taccgaatgc gacaaattca tcgaccaaca
    41461 cccgagtatt cgggtcggat ggatagtttt cgtgccaggc gacggaggga acgaacgccg
    41521 tctccgacca aagaatgata tccgggttcc gggtgagcgc ttccagactg agccgtcgaa
    41581 ggttgttgaa attgttctta taggtcgtat acccaccctt ccatgtatcg gcactgtgtt
    41641 gaacggtggc gacgcgccaa gtcaaatcgg gggtttgtcc gttccaatag gccaagctca
    41701 cgattccgta gatgatggaa gcaaggacaa gaatcccata cccgatgacc ggaatcagat
    41761 ggtctttgat gaaactgaag aactcttttt tccgttcaac gagaaagaac gccaagaacg
    41821 tctgaggaag aaccatcagg aaggtgactc cccaaatacc ggtgatctcg gcgatctgga
    41881 tcaacaccag ataccgatag atcgccgagc ccagcatccc ataggggtag ccggcaaacc
    41941 agttcgttga aagatatgta taggcgacgt agattaccgc atcgacgaga tagctgtact
    42001 tcttgaagaa gcgctggctc agtttcaggg ctggaaagag agcgatcatc tccccacctt
    42061 tgatgatcgg gacaagaaga atcgccaaag ggtggaacgt cttcagccaa tagttgaaaa
    42121 agaggtagaa aacaaaccca tagaagaagc catagggtat gaccagtttc cacgtggtat
    42181 tccgaatcac catgaacatg ggaatcaggg caaagaacgc gatccatcca aatccggtgg
    42241 tgttgatgaa accggggaat gcgagcgaat agacaaaagc gcttgcaata agagcaaaaa
    42301 cctcagaacc cacggttctg aggcttcttc gggatagtga attggtcaag gaaacgctcc
    42361 tggtttagtt atcggtcgaa tcgttggagc ggagatccca gacacactct ttcagttctt
    42421 tgcccggacg gaaaatcgct acgccgtgag tctccacaga aacaagatcg cccgttttcg
    42481 ggttgcgggc tttctcccgg cctttacggg tccgaacttc aaatgttcca aaaccgcgca
    42541 actcgatgac ttgatctttc ttcaggcctt ctttgatttc ctcaaagaac tcatcaatga
    42601 tcgtatggat atcggctcgg ttgatcccgt gcttctcata gagattttct atgatcattg
    42661 ctttggtcaa tttaggattt gccatgctat ctcctccaca acttcagggt tacttacgct
    42721 tgggcgacgt tcgcttgctc cattttgttg aagcgaagct gcaacctgga tttcttgcgg
    42781 ttggcggtgt tcttgtgaat gactcccttg cttgcagtcg agtcgatcaa cttaagactg
    42841 gtcgccagcg ttgcaccggc actttcaaca tctccggaag cgacagcctc gttaaatttc
    42901 ctaatcgcgg ttttcatcgt cgatttggtc gaacgatttc tcagtctgcg cttgcggttc
    42961 tgccgttctc ttttttcagc tgacctgtta atgctcacgt tattcctcca aacgtattca
    43021 acacaatagt taattatata aaaatgataa gcccaaagct cgggcgtcga tggttttaaa
    43081 acatatcaca gtcgctaaaa ctggtcaata caatacttta tttcagttcg ataaaaactc
    43141 cgtgacgtat tcccgcttca cttcccaacg atttgcatga tcgttgaaaa acagcgcggc
    43201 ctggggaaga ttcgcaaccc tgccttcgct tccactgagt gtaatctgct ttgacagtat
    43261 gttcacttcg gtgactttca tcaagtcact gcccaccttc aatcggcttc ctcgaggggg
    43321 ataattctgc ttttcttcat tataaaaatc atattcatag ctgagacaac ataataatcg
    43381 gccacaagga ccggaaatct tcatcgagtt caaggaaaga ttctgttctt tggccatctt
    43441 gatcgagacc ggattcaatg tgtcggtcat tgaatggcag cagtaatcgc ggccgcacac
    43501 cgccaagccg cccagcaccc ggctttcatc ccggactccg acttgccgga gttcgatccg
    43561 aatcttgaag atcgacacca agtctttcac cagctcccgg aaatccaccc ggacatccgc
    43621 cgtaaaaaag aagatgatct tcggttcgcc gagcaggaag tgtgttgtaa ccagcttcat
    43681 gtccagattg cgctcggcga tcttctcgcg gcagattcgc atcgcctcat cttcctggcc
    43741 gcacagctct tggtagcggg ccaaatcgct tggggttgcc aagcggtcga tccattgcac
    43801 atcggtatcg tgcaaggaaa cgatttgagg ctcgaccgag ggaacacacc ctttgcagga
    43861 gaagcattgg tgctcatccg gccggtcgtt gtagatgatc tcatcgcttc ctgcaagctc
    43921 ttcggcgact tcgacttcat cccataattc ggcgtgctcg caacatcctc gcacgttcgt
    43981 atcgcctccg cgatactcct gattgaggcg gttggcgttg ctgaccacca caccaaggtc
    44041 aagaccgtat cgggtcggag ccacgacact cgtacccgga tacaacaccg aatcccaagc
    44101 acaaagagtc gtttcatttg atgccggatt tttcaccaga tatatgtatc cggcagaggg
    44161 gcggtccgac tgttgactca ccctgctccc ccgcaagtgg tctttgttca tatcgctgta
    44221 aaactatcac gcaccactga aattgtcaaa cgcaaactac cacatcgtaa ttgtttgata
    44281 gcttagaaat tgcaacgttg acgactgaaa taccccatgg tattatggtg aaagaattcc
    44341 cgatagcgaa tcttctcaag gagttggtat gaagttattc gacacgcatg ctcacatcgg
    44401 actgatccag gacgatcaaa tgagtcagct gttggcagtg caaatggcaa aggtaaaagg
    44461 cgtcacccat gttgtctcga tcaccaacag cctcgctgat tttgagaaaa cctatgaaaa
    44521 cctcaaatcc aacgattcgg ttctccatgc ggtgggagtc tcgcccacgg aggttctcaa
    44581 cccgggacag aattgggaag agcgggtcat ggccaacgcc aagcgggatc gggtcatagc
    44641 catcggggag acgggactcg actatcacca ctacgcccag gcaaggagcc aacagattga
    44701 gctgatgctg aagcatctgg atatcgcccg gattctggaa aagccgataa tcatccacaa
    44761 ccgcgacgca aaggatgatc tgcttccgat tctctttgaa aagcttccgc ccaagggagg
    44821 catccttcac tgcttcggcg aggattgggc gttcgcccgg aaggtgttgg atcttgattt
    44881 gttcttttcc tttgcgggga accttacctt caaacacaat cggaatctcc atgaggtcgc
    44941 ctaccgattg ccgctggatc gcatcctcat tgaaagcgag gcgccgttca tgacgcccca
    45001 tcgctacaag ggggagcgca acaagattca ctacctcgtt gaaacgttgt atgcgatcgc
    45061 tgaaatccgg gacatggcgg ttgaagatgt ttccgaggtt atctatgata acagcttcag
    45121 ggcattcgcc ctcaagcagg agtcatgacc aggcaccacc cgatattcat agccgatgcc
    45181 ctcaccctca aaggaaacct cttccttgcc cctttggcgg ggtataccga tgctcccttc
    45241 cgccagttgt gcatcgaagg aggggcggat ttcacctata ccgaaatggt cagcgtggaa
    45301 gggcttgccc gaaaggggga aaaaacgatc gcgctgctgc cccgggcaaa agatgagaag
    45361 gagtatgcga tccaactctt cctgggtgag cttgcctccc tcagatccgc tcttgccatc
    45421 gtaaaagagc atcaaccgac gatgatcgac atcaacgcgg gctgccccgt gcccaaagtg
    45481 accaagacgg gagcgggatc ggcattgatg aaaaacccgc cgctgatcat ggagatcgtc
    45541 cggatcatca aggaagagag cggaatcccc acctcggtga agttccggat aggatgggat
    45601 catgagaacc agaactacct tgagttcgcc gatgcggcaa tcgacggcgg agcggatctt
    45661 ctgaccctcc acgcccggac ccgcgcccaa ggctacactc cttttgcgca gtgggactgt
    45721 ttgacctcgt tgaaaaccca tctggcaaag aaaggattga ccattcccct catcggatcg
    45781 ggtgatctct ttgaagcgga agatgcggtt cggatgcttc gcgagacgaa catcgacggg
    45841 gtgatgttcg cccgaggggc cgtcgggaac ccggtaatct tcaaccagac aaaagcgctt
    45901 ttggaaggaa aggaaaaacc gctcttgacc gcaaccgata aagtgaatct cctgctgacc
    45961 catctgtacg cccttgcggg gtactacgat gaacttacgg cctgtgcatt gatgcgcaag
    46021 ttcatcggcg gctaccttaa aggaataccc catgccgcca aagtgaagca ggcggcggtt
    46081 caagctctca ccatcaaagc atatgaggat gccctcaggc atcttgcttt atccgaagat
    46141 cggatatgag aacataagaa taatcagccc tctacgccat ggaattctta tatccggggc
    46201 actcatggct atcgatgtct caaatacaat ttgaccttct ccataggaga atgatagatt
    46261 gaacaaggag gtgatcacat gagtggtgca actgagattt attacaacga acttatgcga
    46321 tttttcggct tgataatgca ggacgaggga gattccatcg ataaagccgc cactctgctg
    46381 gttcaggaga tcaaggatga tcggatcatc aacgtcatcg ggaccggcgg acattccaac
    46441 atcggagcat gggagatgtt ctatcgggca ggaggacttc gatgcatcaa cgccatcctc
    46501 gacccgggta cctcattgga gcccggagca cttcgctcca cgatcatcga acgcgccttg
    46561 ggctatggta cggcggtcct taccgcctat gaggtgaacg agggcgtgct catcatcgtc
    46621 aacgcgtacg gcttgaacac catgacgatc gaggtcgcct tggaagctcg aaaaagagga
    46681 atcaagacga tcggggtgac cagcagaacg ttctccgagt cggtaccgaa ggatcatccc
    46741 gcccgccatc cgagcaaaca gaacctctgt gatgtcgtcg atgtccacat cgatacccat
    46801 atgccggtca tggatgcgac tgttccgatc gacggcctcc tcgcgccggt ggggccgacc
    46861 tcgacgatcc tcaatgcatt ctgcttgaac atgctcgtcg tccgaaccgt cgaactatgc
    46921 atggctcagg gaatcgagcc gccggtgaac atcagcgcca acgtggcgtt cacccccgag
    46981 atggtggaat atacccgccg gatgatggaa aaatacaaac acaggctcta tcactactaa
    47041 aagaggagtg catcatggct tcaaccattc gcgtactggt cttcggggct catcccgatg
    47101 acgatgacct acaatgcggc ggaaccgcga taaaactcgc caacgccggt cacacggtga
    47161 agttcgtttc ctgcaccaac gggggaaccg ggcatcatga aatcggcgga atcgaacttg
    47221 tccgcaggag atatgaagag gcccagaggg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47341 nnnnnnnnnn natgaaatcg gcggaatcga acttgtccgc aggagatatg aagaggccca
    47401 gagggctgca aaagtagccg ggatcgccga atatcaggtg ctggatatcc acaacggcga
    47461 gctcgaggcc tccgttgtga acagaaagac gatcatcagg atcatccggg agttcaggcc
    47521 cgacctgatc atcacccacc gcccgtacga ctaccatccc gaccatcgcg tcacctcgca
    47581 gcttgtccag gacgcgtcgt acatcatgag cgtccccaac atgcttcccc tcaccgaggc
    47641 gatgactgag ttcccggtga tctgctatat gagcgacacg ttccaaaaac caattccgtt
    47701 cagccccgac atcgtcatcg ggattgatga cgtcttcgac cggaaagtcg agatgatcca
    47761 ctgtcatacc tcccagatgt atgaatggct cccctacaat cgcggagtgc tccacaccgt
    47821 tccgaccggc gaagggaaac gaaaggaatg gctcagagag cactttctgg atccccggga
    47881 tcgggccgat cggtatcgga accgcttgat cgagctctat ggcgaagctg aaggaaaggc
    47941 catccggtat gccgaagcgt ttgaagtctg cgaatacggt cgtccacttc aattgactcg
    48001 caccgagatt gaaaacgttt ttgtcctcta gattcgaaaa ggaaacccgc aatgagcatc
    48061 tttgaaacga tcatgctggc ctgcttcggc atggcgtggc cggtgaatat catgaaaaca
    48121 gtcaggtcca aaaccgcccg tggccggagt ctgatgttcc aagccatcat ccttgtcggt
    48181 tacatcagcg gaatcatcca caagcttctg tacaatctgg atatcgttct gtttctctat
    48241 tgcctgaact tcgtgatggt tggaatcgac ggtgccctgc ttctctacta caagagaaag
    48301 gaagcctgtt gttcgtgatc tggttttttc ctaccggata atctttcgta ctcaaaatgc
    48361 agaatcttct ccttggcaat ccaagtcctt gccgtacctt ttggcgctcc ggctcctttt
    48421 gtcgaggctg atgccatgtc tacagatccg gaaatccgat tttccgctcg attgaacaat
    48481 ttttacaacc cataaacacc tccatcgctt cccgaagtcg acccctcctg cgaaagtatc
    48541 aacgtattcg cccgcagacc aacccgccta aatgtataaa attaacctat tagaggcata
    48601 ttaattgtaa ttaatgaata tttatacttt acaaatctat aaaatcggtt attatacaac
    48661 taaatgtgcc caggcactac gcagaacatt gagtatcacc acaccgagta ttgagcatcc
    48721 gtcctacgta tacccttctt agcgaatcac tggagtaatc cagaaaatta aaaaggagtc
    48781 gtatatgaaa aagagtatgt acgtcctatt gaccgtgttg ctcatggcaa ccaccctctt
    48841 tgcggcaggt tcccaagaac agtcgtctgc catcaagatt ggcgtttcga tgcccaccca
    48901 gagcctccag cgctggaatc aggatggagc gaacatgaag agccagcttg aggccgcagg
    48961 atacaaagtc gacctgcagt atgcggggga caatgacatt ccgacccagg ttgcccagct
    49021 tgaaaacatg atcaccaccg gctgcaaggt actggtcatc gcttccattg acggttcatc
    49081 cttgaccgaa gttctgaagc aggcccaaga gaagaagatc tccgtcatcg cttatgaccg
    49141 cctgatcatg aacaccgatg ccgtcacgta ctatgcaacc tttgacaact tcaaggttgg
    49201 aacgattcag ggcacctaca tccgcgatgc tctcaagctc gacagcacac cgggccccta
    49261 ttacatcgag ctgttcaccg gatcccccga cgacaacaac atcaacttct tctttggcgg
    49321 cgccatgtcg atccttgagc cgtacatcaa gagcggcaag cttgtcgtac gttccggcca
    49381 gactaccaaa gcccagtgcg caacccccaa ctggtcgacc gaggaagccc agaagagaat
    49441 ggagaacctg atcaccgcca acggctacgg cccgaagggc acccgcctcg atgcggtcta
    49501 ttcctccaac gactccgtcg ccaacggcat caccaacgct ctggtcgccg ccggctacac
    49561 caaggacaat ttcccgatcc tcaccggtca ggactgcgac aagccggccg tgaagaacat
    49621 gctgcaggga ctgcagagca tgtccatctt caaggacacc cgcacactgg cggccaaggt
    49681 cgtcgaaatg gtcaactcca tcatcaaggg tgaggtggtg tccatcaacg ataccacgac
    49741 gtatgacaat ggcatcaagg ttgtgccgtc cttcctctgc gagcccgttt tcgccaccgt
    49801 cgacaactac aaggctttgt tgattgacag cggctactat actgaagccg atctgaagat
    49861 ctgattctcc ttcgattttt ttgcttgcgg gcaacttcgg ttgcccgcat ctttggaggt
    49921 cctgtttgga aaccttttta ctggaaatga aacacatcac caagatcttt cccggggtca
    49981 gggcgctgaa tgatgtgaac cttcaagtac gcccgggaga aatccacgcc atagtcggcg
    50041 agaacggtgc gggtaaatcc acgctgatga acatcctcag cggtgtctac cccgcttcca
    50101 cctttgaagg tgatatcctg gtgcatggga agtcctgcca attccacacc atcaaggaaa
    50161 gcgaggagca gggaatcgtc atcatccatc aggagctggc actggttcca taccttacca
    50221 ttgccgagaa catgtttttg ggcaatgagc ggggaaggta tttttccatc gactgggaca
    50281 agacatacag ccgtgccgat gaattgctca gtctggtcgg tcttgaagaa tccagcaaga
    50341 cggcaatcaa ggatatcggg gtaggcaaac aacagttggt tgaaatcgcc aaagccctcg
    50401 caaagaatgt aaaggttctc attctcgatg agccgacagc ttcactcaat gataccgaag
    50461 cgaaaaagct acttgacctg ttgctccagt tcaaggagcg gggcatgacc agcattctca
    50521 tatctcacaa gctcaacgaa gtggcatacg ttgcagatcg cattaccgtc atccgcgacg
    50581 gcgaggtgat cacgaccatg gacaaggcca aggaagcttt tgatgagaac aagatcatca
    50641 atgccatggt cgggcgcaat atcagcgacc gctttcccaa gcgaaatgca accattgggg
    50701 aagtcagtct ggaagtggaa cattggaccg tcagccatcc cctctatgag ggaatgaagg
    50761 tctgtgagga tatcaacctc accttgcgca aaggcgaggt cgtcggcctg agcggactca
    50821 tgggagccgg acggaccgag ttcgcaatga gtcttttcgg gaaaagctat ggacgcgaca
    50881 taacgggaac gataaagatc aacggcaagg aagtccagct caacacggtc aagagcgcaa
    50941 ttgagaacaa actggcatat gtaaccgaag accgcaagag caacggcttg attctttcca
    51001 agtccatcat gctgaatacg acgcttgcaa acctgaagga ggtaagcagg catcaggtaa
    51061 ttgatcccga ccttgagctg aaggtggcga tcgatgtcaa tcgcaagctg caaaccaagt
    51121 gctcgtccgt actcgaggag gtgaacaacc tttcgggcgg aaaccagcag aaggtgctgc
    51181 tgagcaaatg gatttttgcg gaaccggata ttcttcttct tgatgaaccg accagaggaa
    51241 tcgatgtcgg ggcaaaatac gaaatctatt gcatcatcaa tcagcttgcg gaagagggaa
    51301 aatccatcct cttcatttcc agtgaaatgc ctgagattct cggcatctgc gaccgcatct
    51361 acgtccttag cgaaggcaga atcgtcggcg agatgtcggg gtccgaggca agccaggaaa
    51421 aaatcatgaa atgcatcata cagaatcaga aaggagacca actgccatga gacacagctt
    51481 aggcatcaaa gataccgtaa agaagaacac catgctcatc gttcttctgg ctacgatggt
    51541 tctcttccaa gtactcatca gcagcagcgg aaggggatcg ctcttcgctc cctccaacat
    51601 ttccaacctc atcaatcaga actcatacgt cgttattctg gcgagcggca tgctgctgtg
    51661 cattctcacc ggtggaaata tcgacctttc agttggttcg gttgttgctc tggtgggtgc
    51721 attggccgga acactggttg tcaatcttca ttggaacatc tatctgtcaa ttttgatttg
    51781 cctgctgaca ggtctggcaa tcggagcatg gcagggtttc tggattgcgt atgtccgcat
    51841 tcctcccttc atcaccaccc tcgcaggcat gttgttgtgg cgcggtgtcg cgcttctgat
    51901 tctcaacgga ttgaccattt cacccttccc cgctgaatac accaagtatt tcaacagctt
    51961 cctccccaat accggagatc ctgcaacggt attttccgta acggtcatcg tcggtgtcat
    52021 catctgtctt gtgtatggat tctgggctct tttcgatcgg tacaccaaga aaaagaaggg
    52081 gtacgcgacc gaaccgtttg ttatgacgct gtccaaagtc attctgcttt ccattgccgt
    52141 cctggtggtc ttcaccctgc ttggaaaaca taagggagtg ccggtgatcc tgattcttct
    52201 tgccatcatc gtgctgggct acagctatta cacttcacgc accgtcccgg gcagacatct
    52261 gtatgcaatg ggcggcaacg agaaggctgc aaagctctcc ggcatcgata ccaaccgtgt
    52321 tttgttcttt gtctacacga acatgggatt cctctccgca gttgccgccc tggtaggtgt
    52381 tgcacgcttc aactcggcgg ctccaacggc cggaaccaac tatgagatgg atgctatcgg
    52441 ctcctgtttc atcggtggcg caagtgccta tggcggaacc ggaaccatcg gcggagcaat
    52501 catcggagcg atcttcatgg gtgttttgaa caacggcatg tccattctcg gtattgacgc
    52561 caactggcag aaagccgtca aaggcattgt agttcttgct gccgtggtct ttgatgttct
    52621 gagcaagaaa cgggtgaaat cgaactgacc tatagaacgc gctttccctg tatgaacacg
    52681 ccctcaatgt cgatatcatc attgaacagg agcaggttgg cgcgttttcc tcgggcgatg
    52741 ctgccgacat accgatcaat cccgatgatt cgagcagggg tcagcgtcgc catccggact
    52801 gaatcaacca atggaattcc ggcaatcttc gttgcagtcc gaaccaaacg atcggccgtc
    52861 gcgatggagc cggcgaagca gcttttatcc tccaccagcg ccaccccgtt ctcaatgtag
    52921 atgacacggc tttcagcagc atcggcaaag gttcttgtcc catcgggcag cttggctcct
    52981 ccccaccggg tcgcatcggt gacaagacag atatgctccg gaccctttat tttatagatc
    53041 aactgcagga actctttggg aaggtgcacc ccatccccga tcacttcaac atccatgcca
    53101 tcgatcagaa aagcggcctc gacgatgcct gctttgcggt aggctccctc ccgatggtag
    53161 gaagaacagg atgagtacag atgggttaca tggcggtagc cgttgttgta cgcctcgaag
    53221 gtctcctgaa gggttgcatt ggagtggccg atcgaggcga cgatgccctt tcgctccagg
    53281 aacctgccga atcccatccc cccttccagc tcgcaggcgg cggtccactt tcggatcttc
    53341 ggatagattt ccagatacgc atcaacctcg cgataatcca cggtgcgata cgttttgaca
    53401 tcttgagccc caagcaccga tcccgaacag taaggccctt ccatgtgaat accctctatg
    53461 tcggggacag cttcaccgcg aaaatccaaa tcggcaaaga ggctgagaat gttaagatac
    53521 tgttcatggg tgaggctggt cagcgtcggg aggatcgagg tcgttcctcc cttcagatgg
    53581 acactcagca catttctgat cgcttcttcg ctcccatcat gaaagcttgt cccgttgccc
    53641 ccgtggacat gggtatcgac aaagccgggg ctcagatacg cccccttgcc atcgatgacc
    53701 tcatgaaact ccccgttcgg atacgcttca gtgccgaagt catcgatcaa gccgtcacgg
    53761 atgaccagac gggcatcgcc catcactctg tccggcatga tgagacggac gtttttgatg
    53821 agcgttctca tccgattacc cctttatggc gccttcgacc atcccctcga caaggcgatt
    53881 ctggaagatc gcgaagatcg tgatcggcgg aatgatcgac atcgtgatcg cggccgccag
    53941 caccccatag ttccagtcct cactcttcaa caatgtcaag gcaacggata tcggcatgat
    54001 cgaatccttc gtcgccaacg atttggaaat ggtgtattca ccccacacgc tgtagaaccc
    54061 ggagacgaag acgatcacca gcgcattctg ggcaagggga agcatgattt tccaccatgt
    54121 ctgcacgacg ctgcagccgt cgatttcggc cgagtcttcc aactcgcggg aaaggttgat
    54181 gaacgttcct cgcatgatga agatggagaa tggaagaatc aacagaagat tcggtagaat
    54241 cagaccgata tacgtgttca agagcccaag attgtactcc atgatgtaaa tcggaatgag
    54301 aatcgccgca agcggaaagg caagcacgaa caggatcgag gccagcagcg cgttgcttcc
    54361 tttgaaagga agcttggcga atgaatatcc tgaaagagcg gagagcagca gggtcacaat
    54421 caccgaactc atcgtgataa tgatcgtatt cagatagtat ttgaggtact tcgcacctcg
    54481 gacaaacacg taatcgtagt tgaccaatga ccatttcttg gggaagaacg ttacttcttt
    54541 gataatctcg atcgactcct tgaaagacgt gaagacgcag aatacgattg ggaggacgaa
    54601 gagcagcgag atgaacagca atgccaacga ttggagaacg gagttgatca gtcgtcgttt
    54661 tctcgccata tcactcctcc ttcctgctta ttttcaagtt caggatggaa aagaccatga
    54721 ccagcgcggt aagaatgaat gcgatggatg aagcgtagcc catctcatag ttcatgaacg
    54781 cttcattgta gatcttctgg taaaggacga tcgtggcgtt gcccgggcct cccttggtga
    54841 gaacgttcgg ggtcacaaag agtttgagac attgcagaac cgcccatatt cccaccacgg
    54901 caaatgtttc cgacaggttc ggaatgatga tgtatatgag tttcttgaac ccgacggcgc
    54961 catcgatttc ggccgcctcg aagagctctg aggagattcc ctgaagcccg gagaggaaaa
    55021 gaatcgtact gaacccgacc tttccccata tactgacgat gatcacccac caccgagccg
    55081 ttttggcatc ggtaagccag ttgtgggcat atttggagag acctacgctg atcaggaatt
    55141 tgttgagaat accgaagttg tcggcaagca tccatgtgaa gagcagacag atcaacgaag
    55201 cgggaacgac tatcggcatg aagattgaag tccgatagaa ccagcttctc ttctttttat
    55261 agaacaggag caatgcaaaa gccaatgaga gaacgatcag cagggtaaac atgatcacgg
    55321 tgaataccag cgtgttcgat aaagcccgga caaactggat atctttggcc gtagcaagaa
    55381 aattgttaaa tccattgaat ttcggtcgtt tatcaaacgc gaaattatag tcactgaaac
    55441 tgagaatcgc cgtatatgaa attggatata cgacaaataa aaacaggaca agaaccgttg
    55501 gaagcacgaa cagcatcccg aacagttcct tttccctctt tcttccccta atagtatttt
    55561 gcatacctat ttatcctaac acgtttcaac cggccggcag gctttcgcct accagcctca
    55621 aatcatatac gaaaacctat tttttcagtg aatcggagag tgccgtcatc ttgttgacga
    55681 tatcccgagc cgaggttttc ggatcgacca aaccttcctg gaagatgaca ttcaggccgg
    55741 tgagccactt tgcctgatcc tgccactgag gaatcctgac gccgttttcc atggaatcgc
    55801 tgagcttctt ccacatcggg gtttcatcca aagctttgtt gaagtgttcc ttgatgacgg
    55861 acattttgcc gaacttggtg aaggtgttga cctggacata ctcgctgtac accccttcct
    55921 tgatgaactg catcgcaagg tcggcgttct tcgatgcttt cgggattgcc atctgacagc
    55981 cgaatgcgaa ggttcccttt cctcccggaa tgggggcaag cgacaggttc tcaattccga
    56041 ttgtggggat cgcttccatc catcgtcccg cagcctcgta gaccatcgcg atctggccgg
    56101 ctttgaagcc gttgcgtccg cctgcgttgt cgacgaaggt tgacgtggag atatatccgt
    56161 tctgaactcc cttctgccac agatcgacca tattgacaaa ctcggggaag tcgaagtcat
    56221 aggtatatcc atcgggtttg gttatatggc ctttttgagc gacgattgca ccaccgatga
    56281 ttccggtcag gttgtttccg aactggattg aagaccctac ctgcgttacg accccgccgg
    56341 catccttttt ggtaagcttc tccgagaact tgtagaagtc gtcccaggtc ttcatgggaa
    56401 gcggctcacc gttcgcatcg accaggcccg cttcctggaa catcttcttg ttcagagata
    56461 ttccatagac ttcgtagatc gccggaacgc acacttggac tccttcataa actccggttt
    56521 ccagagcacc gggctggtac ttggatttgg ggaagtcctt gaagaagttg agttccttgt
    56581 cgaagtcata gatgaggcct tgggcaaggt aggtctttgc catgtacccg ccgtcaagga
    56641 acacaaggtc ggtctcggta ttccccttcg accagtcgat gacgtagttg gccaatacgt
    56701 tctggtcggc cagcgtgatg atttcgacat cgacgtcagg gtggttcttt tcaaagatct
    56761 ctttcgcttt ttccagataa atcttgctga tctgccagga ttccgctgaa atcctcagtg
    56821 tccttctgcc cgtggcttcc ttctcgcctt ttccttgagc aaataccatg cccatcgcga
    56881 gaacgagaac gagcaatacg attgctattc ttttcatctt caatcctcct ttaagatgaa
    56941 tattaaacca tgtcgatgtg gcgaagccga aaaattcgct ctttccatca tcgaactgtc
    57001 accaatgatt ctgtaatgaa gattcctggc catcgcgacg gcaacagccc ggttcgggtg
    57061 ttgccggctc gttgactgcc tcatgcgacc cttcggatcg cggcatatac ccctccttac
    57121 tcctggataa agaataccat gctcctgagc ctgtcaacca tatcactttg ttgcaggaac
    57181 atatcaattt gttgccagca caccataatc tcacaaaacc ctatgattga gaatgatcaa
    57241 taaaggttta ttatacaaag agtaaaatag tttttacctt tatcgtggaa aatatgccgg
    57301 ataccaacaa gcttttttgt tccccttcgg actactgtaa aacaaccgat tgctttgaca
    57361 acacattgca acagtccaac agagcaagac ccgtatgata gccggggtca agatccgggg
    57421 tggcgggaat gacgtccacg ggcttgccgc tgctgtcaat tgtctggaca gcgatgggac
    57481 aggccgacgg tttgcggtag ttctccgtaa acgcgctcag gcatcgttca aaccattccg
    57541 taccgaatgt cgaccatggt cctttcttga gatgctcggt caatgcaatg gcgcgcagcg
    57601 tttccggaag ggaccaccag ggcatcgtat cgtaaggaat cgtcttgctt accgcatcaa
    57661 tccgtttgac aatcccgccc gtctcactga agcccagctt cagattgggt tcaatcagag
    57721 aatcaagtgt tccccccaca tcgatcatcc actcatattc cgtctcttca ttcccataac
    57781 actcaaggta gacagcgacc atctgggcgg cgagaccgat gaactccaga gagtgaccgg
    57841 gatccacaag cactcttcct tcttcatcga ggaacacgtt accctcatca tcaagcattt
    57901 cgacgatgat gccgtccttg atcgaaggcc atcgccccat tgagttcaca tgctttgaaa
    57961 ggatcttttc aacaaaccgc cgcccgatgg cgggggcatt tgaatcccgt tcgagccgca
    58021 taagaagcgt taccgccccg aggcccagca tctccgtacc gaacccggtg gcttttgcct
    58081 ttctcccctg atactgagac ggatggaatc ctatctggtc atttaccagc ttgttcccaa
    58141 taatcgcccg caaggtgtcg gtgatatacc ttcttgaggc acgcctctcc tcttccgttc
    58201 cccagtcatg gttgaatgca tacaaccccc gtgagcagaa gaggtcgctc atggtgaaca
    58261 cgtcggtatc ctctgtcgga tgtgatcgat ccgtcgcaag gaaatagaga tgtccgccat
    58321 tggatcgtcg ttgccgttga aggttagcgc tcaccgaacg gccaagagag atcaactcct
    58381 cctttgcatc gtcagaaggc cagcccaccc gatcatacca cttcaggtga gttgcgatcg
    58441 cctcgagccc gcgaccttga atccagctgt agatatggtt cttgccgcgg atgccgtcgc
    58501 tttcgaacat atcttcaccg ctgatggcat caagcttcag gtcaatccat ccatagtcag
    58561 gccgaagagt ccggcgctga agcatcaaca tcatcgtgtc gtctatgatc tgtttcatga
    58621 ccttcaattg cgtcctgcta tcagccggat gcttgattgt tcccacaaca acctcctgtt
    58681 tcattaatca aacctatctc taccattaag cccacatagt cactgattgc agaaccaccg
    58741 acactgttgc ctccgcacgg aaaagaaagc ccatgcaagc caagccggtg gagcacgtct
    58801 ctcaggtgtt gaccggcggc aagataaacg gaatgcttct tcccgggagt ctgaatcgtc
    58861 aacacattct tgcgttccat caactcacta taacatttct catcactacg ttgttaacaa
    58921 aaaaatcctt tgaatgtcgg acatctatgg cgaatgtttc aacaccccgt cagatgagac
    58981 gaggaaccta aggagagaga agcgagcgaa ttgtttccct gcgagtctcc actccttgtt
    59041 gaccactgaa gcgctaaaat ggtagcttta ggacaatgat tttcaagaag gtaattcgat
    59101 gagagtatta gtattgggct ccggtgccaa ggatcatgcg atcgcatggt ggttctctca
    59161 gagcaggctc atcagcgctc tctatgttgc cccgggaaac gccggaacgg aatcgatcgc
    59221 caagaatatc gagatcgatc catccgatcc cgaagcggtg taccgcgtgt gtcaaacgca
    59281 tacgatcaac tacgttttcg taggaacgga agcacctctc ttcaccggag tgatcgacta
    59341 cctcaacgaa cggggaattg ataccttcgg agctcccaaa cgagccctcc ccttggaagg
    59401 ggatcgcgac tttgcccgta aattcactga acgacacagg attcccgcac cccgttacgt
    59461 gctctttcgc aacaccgatg atttaagcgc ctatctcaag gaacatgaag gcgagcgttt
    59521 cgtcgtaaag agcaatcaga tcgctccttc ccgggtgatg gtggattcca ccgattacac
    59581 cacgttgatg gagttctccg ccacgcttct tgaaagcggc ccgatcatct tggagcagca
    59641 ccttgcaggc cttcctctta cgattaccct cttcgtcgat accaacagct atatgctgct
    59701 gccgatctgc agtgactaca tgcgcgttga aagtggtgga actccaaccg gcggcatggg
    59761 atcgatctgc cccgttcccc ttcaggattc ggtgaaggaa gcgttgatct caaggatcat
    59821 tgagccgacg ctcaacggac tcagggaaga gcatatggcg tacaaagggg tgctgacaat
    59881 cagcatcatc ctcacgaaag agggtccgat gctcgttgat taccacgttc gcttcaatga
    59941 cccggccgcc caagcattta tccccctcat ctgcaccgat ctcatcgaga ttctcactgc
    60001 catgaaaaag gataccctcg gatcgctgaa actgtgttgt tcctccaaca gcgcggtcgc
    60061 cctcgtcgtg gcgagtgaag gatatcctca aaagcccgtt accggaaaaa tcctcaatcc
    60121 gattcccgcc gccctcctcc tcaatgcctt tgaaggagca ccaaggtact tcttcggcgg
    60181 agtccagaaa cgaggcaacg accttgtcac caccggagga cgaaacgtta ccgtcgtcgg
    60241 aatcgattac aatatcatga gcgcgaacaa acgcgcctat cagggcgtcg ttcatgtcca
    60301 gttcgagaac gcctggtacc ggcaggatat cggaaacaag ttctttgaga actgagaaga
    60361 cctctctgtc attcaataaa ggcgtcttcc tcataataaa atgagtaatc gatacctttc
    60421 atgaacatcg ctcgatcatc aatcagatcg gtgagcgcct caaaaagaag aatcttcaga
    60481 ggatgagaat ccaccacact cgcctccatc gcatcaagat atgagcgttt ttcgatcttg
    60541 ctccaatcga tacattttcg aagccttttc ttgaggatga gatccaacca gatccgggtg
    60601 cttcttccat tcccctccat gaacggatgg acgatgttca tttcaatgta tttcgtcaaa
    60661 ggattgttcg ctcatctgct ctttcttctg caattcctga tgaagaaact gagccatcgc
    60721 gaactggaag ccccccttgg cgatgttacg agtgcgaatc tgtccggcaa agtcgtataa
    60781 tcctccaaac agataggcat ggatctattg aaatccgttg acggttccca cctcgatcga
    60841 ctcgagcagc gaactgtcaa acagtgagta cgctttcgct ttgcttagtc catcgatgga
    60901 ctcttcacca taggtgaacc actgaagaaa ccgattcgct tgcttaccgg gaaattgctt
    60961 tgccaattcc ctgatacctt caccatccag aacgttggcc agccgtagct tcccgtcgcg
    61021 cgcggtgaat tgaaggggct gtcgcaaaag gtcgtaaggc ttgcggcagc tccttttatc
    61081 tgtttattgt taaagcattt ataacgaaaa gaggttgcaa aagcaacctc agtgtgctat
    61141 aatttgttta cctacaaaca attaggagca catatgaata gtacagcttt ctttgaaaat
    61201 ggtcaactct ttcttaacct ttccatcccc ctcaagcttg atttccagaa tcccgtcaaa
    61261 gcacttcata ccctgttgtg gtcattggac tatagttgct tctcaaagca gaagaaggtc
    61321 agcggcagac cgaatgcact ctcgcctcga aagatgatgt tcatccttgt gtatgcgagg
    61381 ctgaacaact gtttcagcag cagggaggtc gaagccctgt gcaagagaga tctggtatgc
    61441 atccatgtct tggagggagg cagggctccc gaccacagca ccatcgaccg gtttgtcagg
    61501 ggcaacgcag gacccatcag ggatatattc gctcagagta ttcgcaagct cgacgaactg
    61561 ggagagttgg gaagaaagat ggccttcata gacggaacga agatcgaatc gaaagctggc
    61621 aagtatacgt tcgtatggct gagcgccata gagaagaacc ttcccaagct ggtaaggaat
    61681 atcgccaagc tctatgggca gtacgtagag cactatcgtc tggacaaccc atcagttgtg
    61741 gagaccgaga ttcaagccaa ggtggcactg gatgaaatga tagaagtcat agacccgcat
    61801 gaggttctca gccgtccctg taagcaaggc cgcggacatc gcaaaggctt tgagcggatg
    61861 ctctctgaga gacttctgga atactcacgg aagcttgcct tctaccgaag agcccggaac
    61921 aggatgaaag cggagggacg gagaagcatg agcaagaccg atgaagatgc gaccttcatg
    61981 agaatgaagg aggattacat gcgcaacgga cagctcaaac ccggctacaa catacagaac
    62041 atcgtcgatt cggggtatgt agtgggaacg tattgcagca gcgatcgtac agacatgtac
    62101 acattcgtac ctgccttgga gcagttgcga aagcggttgg ggttcgagta tcaagggata
    62161 tgcgccgaca gcggttatga ctgtgtggag aactaccgat acttggaaag caggggcatt
    62221 gatgcctaca tcaagacaca gacctatgag cagaacaaga agcgcaagat tcgtaaggat
    62281 cctgggaaca agctgaacat gacttacgat ggggcaaaag accggcttcg gtgtatgaac
    62341 aacgccatct tgcataacac gggcaagaag aaatccgacg gaacatcttt gtatgaggcg
    62401 aagaaagggt gtgtaagctg tacataccgc aacatatgca tgaaggggca agccacacag
    62461 aagaacttca agcggatgca gtacaatccg gttgcagaag gtttccgttc aaagtcgctt
    62521 tcaaacatcg taagtgagaa gggcatagaa gcaagactca acaggagcat ccaagtcgag
    62581 ggttcatttg cactgattaa ggatgccctc ggtatacgca ggtttctcaa caggggaatg
    62641 gtcaacgtgg aaaccgaatg gatcatcctg tgcatggcag ccaatacgct taagctgcag
    62701 gcaaagatca acagaggatg tttgggtatt cccacttggt accatataga taaatccgac
    62761 cctttggatg aggctggtta gacaaaccta aacaacaaca aacaaccaac aatatctcag
    62821 ctgagccaag ggatagctat gtccataaag ggagctgtct ttgtcatttc tcagctttca
    62881 atgcttcatt tccttaagat ttgtttgttg ccgaccattc cgattgccgg acataaaaag
    62941 aggccgtcgc tttttcgact tttgcgacgg cccct
//
LOCUS       DAOSLI010000005        53104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_726_length_53104_cov_31.175310, whole genome shotgun sequence.
ACCESSION   DAOSLI010000005 DAOSLI010000000
VERSION     DAOSLI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 53104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53104
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_726_length_53104_cov_31.175310"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1134)
                     /locus_tag="PLF67_01560"
     CDS             complement(160..1134)
                     /locus_tag="PLF67_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOE88732.1"
                     /translation="MKKILTFLMILVLVTGTLFAQAQAETIDDFPSKPVTAVIGWSAG
                     GGADIVFRALAEVFPKYANGQPLVIKNEGAASGVPAITAFKSATPDGYTIMHWNVAHL
                     IKTHMTKVAFTATEFEEACQVVASYNYLVVNADAKWKTLDDFIADARKNPEGISMGNA
                     GTGGGNHMAALLFEGATGTKYVHVPFSGGGPAITGLMSHQCDAVMAIAPEGISNVQGG
                     QLRILAVFSDKQIDGIPAKTGREQNIDLVLEQWRGVIAPTGTPPEILKKLENIFKQCV
                     ADPAYIAKMKELGATPVFKSGAEYSKIVEADDARYEKIIREGGFGDRY"
     gene            1285..2481
                     /locus_tag="PLF67_01565"
     CDS             1285..2481
                     /locus_tag="PLF67_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOE88733.1"
                     /translation="MQNDDLEFINCANVLKAIGTDLTQPSRAQIAKYLGLSRTAVSMV
                     VKKLMAAHLISELETEKSGRGRPGTPLVLDGTHWHAIGAAYSSKLWTFCVVNLNGEPL
                     LTHKEYVETTDQDETVKALLQGLGHVESKVNKPLLPGYGIGSPGLIDPKSGTVFRADD
                     MGWTKPLPLKQLVEEATGKKAYIINRYRANGVAELTFGGHDLTKNIIYLGVGTGIAGS
                     VYLEGTLLNSTKYRFGHMVIDPHGPLCGCGQYGCLQAMASEKALLTYARERMKEDLTF
                     LAAYADQPITAKLLVDLAEGGDPHAQSCLSYIASALGIAILTLANVIAPDEIVIGGPI
                     GDASTFLVDEVRKAAYAHLLDWQVSSLKISKGSQGDYGSVLGAAAFLLEQKMELLLGE
                     GCPVIS"
     gene            complement(2478..3527)
                     /locus_tag="PLF67_01570"
     CDS             complement(2478..3527)
                     /locus_tag="PLF67_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE88734.1"
                     /translation="MKKLGFGIIGLGAIADFHARAIKELDGCELVNCYHYREERANEF
                     AKKHGCTAYTDLDKFLADDRLDIVAIATPSGAHLDGALAAAKAKKHVIIEKPLEITKD
                     RCTQIINACKEHNVKLAGIFQSRFFDSSKILKEAIEKGRFGKIVMADAQVKWYRSQAY
                     YDSAAWRGTWALDGGGALMNQSIHAIDLLLWLVGDVSEVFAFADTLSHERIEVEDTAV
                     AVLRFNSGAMGVIEGTTSAYPGFLKRLEILGTKGSAILEEESIIAWQFEDEEPGDEEI
                     RKTFLDSTETGGGAADPLAIGHHGHTRNFASFVKSIREDSPVEVSGLEARRSVELIEA
                     IYRSAKSGKKIELPL"
     gene            3681..4583
                     /locus_tag="PLF67_01575"
     CDS             3681..4583
                     /locus_tag="PLF67_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88735.1"
                     /translation="MDLFPALLAIIISGFAGGIFSFGYKAIVQLKQRTGPFIFLYGLT
                     YTIFAAVGILLIGGVSADPIVWLFGVSHGVIMCMAILFYIRIIGKIRLSISWTIIQFS
                     IFIPLVTSLLFFEKHLSPLSVSGTVLMLVAIIIFSFGKKEHSSKPEGPPISLASWLLL
                     FLSSLFSGMALTIPMVYMKAATASQPLYLIFASGVGTLLCSLLFIRKEDWKYMKGDWK
                     VVGVPSTMSLLQIMSWAFLMVGLLDLDGAVAYSLKGFIGLLCVYTVSFSFFRETVTRI
                     EKIGILVSLGAILLMTVSLYTVAL"
     gene            complement(4597..5427)
                     /locus_tag="PLF67_01580"
     CDS             complement(4597..5427)
                     /locus_tag="PLF67_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88736.1"
                     /translation="MKIIEQLCMGKSPDLENEDGLFINEHFAAVIDGCSSRKPLSGQT
                     RTNGVIAKNLLLACLENLAPHASKDETFHALNDSIRRWYGTRDLLEAAEEDAELRPGA
                     YIAMLSNARREIWILGDCQALADGVHYTETKAIDTLMELNRTMLIEEALIQGFSREHL
                     LRHPELIQDRLSEFMTHQAAFQNRMVGTSIFGYPTLDGFFDHYESIIVVQLGSDSKEV
                     VLASDGYPFLRPTLEESEMKLKQLLQKDPLLYTEYRATKGLNPAYTSFDDRSYLRIRI
                     "
     gene            complement(5424..6326)
                     /locus_tag="PLF67_01585"
     CDS             complement(5424..6326)
                     /locus_tag="PLF67_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE88737.1"
                     /translation="MNRILGLSLVGFREREAIQAHQEQFPSLQWELSYKMSEQFLSDV
                     EPIIADRVVSVHACCPAEPLFPNFGSRDKTVLDESFEALKRTFKTAQRFGAKIVVLHP
                     GYGTDMEIPASNAERETLLTGPSFAPYVGVEKGSICRADYPESEVYRQHFEQAKEALV
                     RLGAMARGAGLQLAAENLNPRVAYLAQTPKEMVSLARASDDLFLCLDVGHLWIAGQVY
                     GFDYLEGIKEILATKKVITAHVHSNPSDRGRGIFVDSHDSIDQHSFDFRTVLKLLADS
                     GANIILETVNSPKRNTLLLYEALG"
     gene            complement(6323..7090)
                     /locus_tag="PLF67_01590"
     CDS             complement(6323..7090)
                     /locus_tag="PLF67_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOE88738.1"
                     /translation="MNSTLRKREILALLEEHGSVQVGDLANRMGVSKVTIRNDLDELA
                     TLGSLVRTHGGAIIAENKGSSRYISNTIHEFRKEKEAIASLAVTFVKDGQSIIIDNGS
                     TTMHIASHLAGRHVTVVTSSLLVMKELMDVESVDLMMAGGILRRPAMGLMGAFSKDFY
                     RQIHADWYFMGGSGYLSEQGVFCTNLIEADSKRSMIESAAKVVFLADSSKMEHPSLAK
                     VCDWSVVDYMITDRIDAEVRSVIESHGVKVVVVGEPK"
     assembly_gap    7228..7327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7405..7842)
                     /locus_tag="PLF67_01595"
     CDS             complement(7405..7842)
                     /locus_tag="PLF67_01595"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE88739.1"
                     /translation="MVTPDCFTREHIENIKSGFTVDSIILERSIFALALLEALVKVGM
                     PFIFKGGTSLLLLLEKSRRLSTDIDIIVKPGTDVQSYLEQAGEIYPFRSMKQQIRTGR
                     NKIDKAHYKFIYDSPGMNTSFHILLDILFEENHYCTERLTIPS"
     gene            complement(7846..>8552)
                     /locus_tag="PLF67_01600"
     CDS             complement(7846..>8552)
                     /locus_tag="PLF67_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88740.1"
                     /translation="PDGVCSRQQILLAARSYDPSFKETQLRYLTGALLDSDLVVRVGR
                     NQYKKAGEEPKKRIFAGKYSNAAMQVIRYMEKHFPLLSFRVWELRWLNEFFNHQLAHN
                     KIFLEVEKDGFDFVFSALIEKFPGRVLLRPGVKEILQYGTDDGVIVERLVTEAPSAGG
                     ERYHVPLEKLIVDLFANRYLMLFKGEYPSTIEMMFSTYRIDQVAMLRYARRRNKVKDV
                     FGFLSTKTTVECMVQW"
     assembly_gap    8553..8652
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8885..9283)
                     /gene="rplQ"
                     /locus_tag="PLF67_01605"
     CDS             complement(8885..9283)
                     /gene="rplQ"
                     /locus_tag="PLF67_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOE88741.1"
                     /translation="MKHRIGFNALSRNAAHRKAMKRNMVTSLFRYERIETTKAKALEV
                     RRMAEKMITRAKNDSVHNRRQIARDISDKAVLNKLFTEIAPLFTERNGGYTRVLKTGN
                     RLGDAAEMVILELVEKPVKKETKKKAAKKD"
     gene            complement(9273..10325)
                     /locus_tag="PLF67_01610"
     CDS             complement(9273..10325)
                     /locus_tag="PLF67_01610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOE88742.1"
                     /translation="MARKNLLKGFKKPKGITFEHSAVEPNYGKFIAYPFERGFGTTIG
                     NTLRRVLLSSIQGYAVTAIKFTSFNEDGVPHLISSEYEQLPGVREDISDIIAALKKLQ
                     IQMPEDAEGTNLLIECKGPGVVTGAALERDQVEIINKDLVLFTMMDDANLEMEVQIDL
                     GRGYVPSEINEKYIEEIGTIPLDATFSPVTRVKYSIEPTRVGYRSDYDKLIIEVYTDG
                     TIVPQNALAEAAKIAKDYFQIFINFDESLISSNDEVDEEEERIRKILNTSVEELELTV
                     RSSNCLKNANIRTIGDLTKKSEDEIAKTRNFGKKSLQEIKEKLKEWNLSLGMTDYSIL
                     KTAIKVPEHKEEENEA"
     gene            complement(10335..10979)
                     /gene="rpsD"
                     /locus_tag="PLF67_01615"
     CDS             complement(10335..10979)
                     /gene="rpsD"
                     /locus_tag="PLF67_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOE88743.1"
                     /translation="MARYTGPKCRYCRAEGTKLFLKGNRCLSGKCPLNSSGKRTGLPG
                     KDPRARSKKPTNYGLQLREKQKLKRTYAMLEKQFKLTFNEAARIPGKTGENLIQLLEQ
                     RLDNVVFRLHFASSRYQAAQFVNHGHIFVNGKRVDIPSYRVKVGDEISISPRAQKMLI
                     IKENLKEYTKSGVYQWLSLDVDAMKGTFLAVPRRSEVTELEKINEQLIVELYSR"
     gene            complement(10997..11374)
                     /gene="rpsK"
                     /locus_tag="PLF67_01620"
     CDS             complement(10997..11374)
                     /gene="rpsK"
                     /locus_tag="PLF67_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOE88744.1"
                     /translation="MATVKRKTKKTVFEGNVYIQATFNNTIVTVTDLQGNAVSWASAG
                     GLGFRGAKKSTPYAAQTTAEKAAKTAMDSGLQEVNVFVKGPGVGRESAIRTLGVLGLK
                     VRSIRDITPIPHNGCRPRKSRRV"
     gene            complement(11389..11754)
                     /gene="rpsM"
                     /locus_tag="PLF67_01625"
     CDS             complement(11389..11754)
                     /gene="rpsM"
                     /locus_tag="PLF67_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOE88745.1"
                     /translation="MARIAGVDLPNKATKIALTYIYGIGRYSAIEICEKTNIDPDINI
                     NTLSNDDLAVLRKVIEEEYKVEGRLRTEVALNIKRLMDIGCYRGLRHRRGLPVNGQRT
                     KTNARTRKGRKKTVAGKKK"
     gene            complement(11793..11906)
                     /gene="rpmJ"
                     /locus_tag="PLF67_01630"
     CDS             complement(11793..11906)
                     /gene="rpmJ"
                     /locus_tag="PLF67_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOE88746.1"
                     /translation="MKVRSSVKPICDKCKVVRRSGVVRIICENPKHKQKQN"
     gene            complement(11924..13243)
                     /gene="secY"
                     /locus_tag="PLF67_01635"
     CDS             complement(11924..13243)
                     /gene="secY"
                     /locus_tag="PLF67_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOE88747.1"
                     /translation="MANTLVEMYRIKSLRKKIFITLGLLVVSRIGAVIPIPGIDPQVL
                     KLFFLAQSSSSTIGLTEYLNFFSGGAFSNFSLFMLGVMPYISTQIILQLLMLVIPSLK
                     KLASDPSGHKKLQQYTRYGTVVVCLLQSYVVTIYAQSIPGVLTMGLGSFTLIAMLTVT
                     TGSMLLIWIGNKITQWGIGNGISLLIFAGIVARFPTAVNALFEAIKGGVLNPIVVLIV
                     AVMFLIVVALVVYEEQGIRKIPVNYAKRVVGRKMYGAQSTYIPIKVNPSGVIPVIFAS
                     ALLSFPLQIATTLGPEVRWLAAFANWLNPQGTPYLIIYALLIVAFAFFYTQVSMNPVE
                     MAKQIRENGGSVPGVRAEKLEEYLTKVLNRIVLPGSLFLAFIALIPTLVQKFFNFPST
                     VAMLFGGTSLLILVGVELDTIRQIEGIMKMHHYDGFKIGQKKKTKHF"
     gene            complement(13255..13686)
                     /gene="rplO"
                     /locus_tag="PLF67_01640"
     CDS             complement(13255..13686)
                     /gene="rplO"
                     /locus_tag="PLF67_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOE88748.1"
                     /translation="MAKIIAPKGANTKKTILGRGASSKGRTSGRGHDGQNSRSGGGTR
                     PGFEGGQMPLYRRVARRGFSNEPFKVVYEVVSLDDISRVFNDGDVVTLAALKELGVVK
                     GVKTQAKILNNGELTKKVIIEGLKVSASAIEAIKAAGGQIR"
     gene            complement(13688..13885)
                     /gene="rpmD"
                     /locus_tag="PLF67_01645"
     CDS             complement(13688..13885)
                     /gene="rpmD"
                     /locus_tag="PLF67_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOE88749.1"
                     /translation="MAKAKKQKQIKITLVRGLAGTLPKQRRTVKALGLGRISSSVVHD
                     ANPVILGMVNVVSHLVKVEEM"
     gene            complement(13889..14416)
                     /gene="rpsE"
                     /locus_tag="PLF67_01650"
     CDS             complement(13889..14416)
                     /gene="rpsE"
                     /locus_tag="PLF67_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOE88750.1"
                     /translation="MDRNRDRRDRDRDREKSDGLTEKLIKINRVAKVVKGGRRFSFSA
                     VVVVGDQNGKVGYGFGKANDITEAIRKAVERAKSAMIQIPMKNSTIPHEIIGKYKGAS
                     VLLKPAVPGTGVIAGGTVRAICDAAGIHDILSKSLGSKNHINTVKATFVALENLFNAR
                     EVAKNRGKSLNEMWG"
     gene            complement(14426..14785)
                     /gene="rplR"
                     /locus_tag="PLF67_01655"
     CDS             complement(14426..14785)
                     /gene="rplR"
                     /locus_tag="PLF67_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOE88751.1"
                     /translation="MKRVIDKQRKLQQRKRHIRKRISGTGTKPRMSVYRSNTHMYVQV
                     IDDVAGVTLVAASTVEPELKDLKNNVNDALKLGEAIGKRMLEKKIDTCVFDRNGHLYH
                     GIVKSIADGARKAGIKF"
     gene            complement(14804..15346)
                     /gene="rplF"
                     /locus_tag="PLF67_01660"
     CDS             complement(14804..15346)
                     /gene="rplF"
                     /locus_tag="PLF67_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOE88752.1"
                     /translation="MSRIGNNPIALPKGVTVAVVDGMIHVEGPKGKLNFKSREEVTLA
                     IEEGKVAVKRVDDSQAARAYHGLYRQLVVNMVEGVSKGFSKTLIINGVGFRADLKGNV
                     LTLSLGYAQPIDFVVPEGITAVVETPTKIILSGIDKQQVGQTAAELRSLRKPEPYKGK
                     GIRYEDEVIRRKAGKTAGAK"
     gene            complement(15357..15755)
                     /gene="rpsH"
                     /locus_tag="PLF67_01665"
     CDS             complement(15357..15755)
                     /gene="rpsH"
                     /locus_tag="PLF67_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOE88753.1"
                     /translation="MAVSDPVADMLTKVRNANMARHEKVDVTTSKMKLQIVKILKNEG
                     YVKNFKKVTKDGISYIRIFLKYDEKQAPVLHGLERISTPGRRVYTGYRDMPRIYNGHG
                     VVVVSTSHGVITGKKATENKVGGELICSIW"
     gene            complement(15765..15950)
                     /locus_tag="PLF67_01670"
     CDS             complement(15765..15950)
                     /locus_tag="PLF67_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOE88754.1"
                     /translation="MAKKSMIIKAKREPKFSTRRVNRCRVCGRPRGYMRQFDMCRICF
                     RKLASEGQIPGVTKSSW"
     gene            complement(15963..16514)
                     /gene="rplE"
                     /locus_tag="PLF67_01675"
     CDS             complement(15963..16514)
                     /gene="rplE"
                     /locus_tag="PLF67_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOE88755.1"
                     /translation="MEKFVPNLKRQYKESVSAQLLKEFGYTSKMQIPALEKIVVSVGV
                     GEAIVNKKLLDSAVKELEQITGQHVVKTKARKSIANFKLREGMEIGAMVTLRGDNMWY
                     FLERLISIALPRVKDFRGVKPNAFDGRGNYSLGISEQIVFPEIDFDKIERVSGLNIAI
                     VTTAKTDEEGYALLKHLGMPFAK"
     gene            complement(16514..16819)
                     /gene="rplX"
                     /locus_tag="PLF67_01680"
     CDS             complement(16514..16819)
                     /gene="rplX"
                     /locus_tag="PLF67_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOE88756.1"
                     /translation="MKLKKNDTVLIIAGKDKGKSGKIVKIDHEKQRVIVQGANMVKKT
                     MRKKSAQDKGGIVELEAPIHVSNVAYMTGKGQTSKIGYKFDESGKKVRYAKKTGEVI"
     gene            complement(16831..17196)
                     /gene="rplN"
                     /locus_tag="PLF67_01685"
     CDS             complement(16831..17196)
                     /gene="rplN"
                     /locus_tag="PLF67_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOE88757.1"
                     /translation="MVQMQSYMNVADNSGAKTVQVIKVLGGTHRYTAGVGDVVTVSVR
                     SALPNGAIKKGEVMRAVIVRTKKEYRRPDGTYIRFDDNACVIIDANNNPRGKRIFGPV
                     ARELREKYMKIVSLAPEVL"
     gene            complement(17212..17463)
                     /gene="rpsQ"
                     /locus_tag="PLF67_01690"
     CDS             complement(17212..17463)
                     /gene="rpsQ"
                     /locus_tag="PLF67_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOE88758.1"
                     /translation="MQKVRKSYTGVVVSDKMEKTIVVELSSRKLHPLYKKYLTRTKKV
                     KAHDERNEAGIGDTVRIVETRPLSKDKHHRLVQIVEKAR"
     gene            complement(17478..17684)
                     /gene="rpmC"
                     /locus_tag="PLF67_01695"
     CDS             complement(17478..17684)
                     /gene="rpmC"
                     /locus_tag="PLF67_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOE88759.1"
                     /translation="MKHSYKEMTLEELVAKRDQLHKEYFDLRMRRVLGHVENPLAVRT
                     IKRNIARTNTLIREYELGIRKSAK"
     gene            complement(17684..18100)
                     /gene="rplP"
                     /locus_tag="PLF67_01700"
     CDS             complement(17684..18100)
                     /gene="rplP"
                     /locus_tag="PLF67_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOE88760.1"
                     /translation="MLSPKRIKYRKKQRGSIDGIATRGNTIAFGEYGLLALEPKWITN
                     RQIEAARVAMTRHIKRGGKVWIRIFPDMPYTKKPAETRMGNGKGNPEGWVAVVKTGSV
                     MFEMGGVSEELAREALALAGSKLPIKTKFVSRREVQ"
     gene            complement(18104..18814)
                     /gene="rpsC"
                     /locus_tag="PLF67_01705"
     CDS             complement(18104..18814)
                     /gene="rpsC"
                     /locus_tag="PLF67_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOE88761.1"
                     /translation="MGQKVNPIGLRLGINKTWKSKWYVDPREYADTLHEDLKLRKALL
                     TSPEASGAEISDLEIIRKPQRITMVITTSRPGVIIGSKGANVEKLGERLQKLTDKKIQ
                     IKIKEVKKPEADAQLIAMNVARQLVARGSFRRAMKIAVSKAMQSGAQGVKIRLSGRIG
                     GAEIARSEWMKEGRIPLHTLRSDIDYGFATADTSFGVVGVKVWVFNGEIYDRPVKDEA
                     GDLVRRSSRNEGLEARSL"
     gene            complement(18815..19189)
                     /gene="rplV"
                     /locus_tag="PLF67_01710"
     CDS             complement(18815..19189)
                     /gene="rplV"
                     /locus_tag="PLF67_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOE88762.1"
                     /translation="MARKEEGGKGYTATAKYLMISPLKVRPVADLVRHESYVNAVSIL
                     EAMPQKGAGLILKVLKSAAANATYLNKKIDEEDLVITDLQVNEGPRLKRVWPRSHGRR
                     DILLKRMSHITVSVDEKASGRK"
     gene            complement(19192..19467)
                     /gene="rpsS"
                     /locus_tag="PLF67_01715"
     CDS             complement(19192..19467)
                     /gene="rpsS"
                     /locus_tag="PLF67_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOE88763.1"
                     /translation="MARSIKKGPFVEKKLYKRVQDSLKANQKQMIKTYSRSSTIIPEM
                     VGFTISVYNGKTWVPVFITENLVGHKLGEFAPTRIFRGHASDKKKGR"
     gene            complement(19477..20301)
                     /gene="rplB"
                     /locus_tag="PLF67_01720"
     CDS             complement(19477..20301)
                     /gene="rplB"
                     /locus_tag="PLF67_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOE88764.1"
                     /translation="MALKTYKPYTPTLRQKSSLDYSEITTNKPEKSLTVGLSRKAGRD
                     NFGRISVRRRGGGHKRAYRIIDFKRDKVGVPGVVRTIEYDPNRTANIALVFYADGEKR
                     YILAPKGVKVGTTVLSGPEAPITVGNALPLKAIPLGLEVHNVELNLGKGGQMVRSAGG
                     SATVAAKEGDYVTLRLPSGEMRMVFGECYATIGSLGNEDHMNVNLGKAGKSRHLGRRP
                     KVRGVAMNPIDHPHGGGEGKTSGGRHPVTPWGKPTKGSKTRSKKKPSGKFIVKRRK"
     gene            complement(20316..20618)
                     /gene="rplW"
                     /locus_tag="PLF67_01725"
     CDS             complement(20316..20618)
                     /gene="rplW"
                     /locus_tag="PLF67_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOE88765.1"
                     /translation="MRADQIIIEPVLSEKSNLAREAEMKKYTFRVHMDANKHMISDAV
                     SKLFKVDVVKCNVMIVKGKPKYSRSRGGSIKGTTGNWKKAVVTLAKGDSIQAIEGV"
     gene            complement(20615..21259)
                     /gene="rplD"
                     /locus_tag="PLF67_01730"
     CDS             complement(20615..21259)
                     /gene="rplD"
                     /locus_tag="PLF67_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOE88766.1"
                     /translation="MKTKVYSIDGKELRTIELDDTVFNREVSNGTIYYAVNNELANRR
                     VGTASTKTRGEVNYSNAKPYKQKGTGNARAGDKKSPIWTGGGTIFGPKPRDYSYRLPK
                     KMKRLAMKSLLSLGVQDGRLVVVEDFTIESGKTRDLSAILKNFVTDESRTILIIGEND
                     ELLRRAARNIPYLYVLSYNSLSAKELLYGRKLLVLEGAAKNLNTFYGEAEGQSK"
     gene            complement(21270..21890)
                     /gene="rplC"
                     /locus_tag="PLF67_01735"
     CDS             complement(21270..21890)
                     /gene="rplC"
                     /locus_tag="PLF67_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOE88767.1"
                     /translation="MLGLIGKKIGMTQVFDAKGILTPVTVIKIEGNVVVANRSEEKHG
                     YNAALLGSIDKKKSTTTKPYAGQFKDVCEPKQHLVEFRDFDADVKVGDVLGVDIFNDI
                     SFVDVTGTSKGKGFAGGVKRHGFGGGRGSHGSKFHRGIGGTAMSATPARTFKGTRMPG
                     HMGNERTTVQNLKVVKVDEELQVLLVKGAVPGPAQSVVIVKKAVKK"
     gene            complement(21908..22216)
                     /gene="rpsJ"
                     /locus_tag="PLF67_01740"
     CDS             complement(21908..22216)
                     /gene="rpsJ"
                     /locus_tag="PLF67_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOE88768.1"
                     /translation="MAKERIRVRLRGFDVELVEQSSKAIVETVVRAGATVSGPVPLPT
                     RINKYTVLRSPFVNKTSREQFEMRTHKRLIDILEPTSSVMDALMKLELPAGVDVEIKQ
                     "
     gene            complement(22280..23470)
                     /gene="tuf"
                     /locus_tag="PLF67_01745"
     CDS             complement(22280..23470)
                     /gene="tuf"
                     /locus_tag="PLF67_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOE88769.1"
                     /translation="MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITQHCARMFGDTA
                     LAYDAIDNAPEEKERGITINTRHVEYQSKARHYAHVDCPGHADYIKNMITGAAQMDGA
                     VIVVAATDGAMAQTREHILLARQVGVPSLVVFINKTDQVDDPDLILLVEEEMRELLTD
                     QGFDGENAPVIAGSAYLALTDPDNPEATKCIDELLDAMDEYIPLPDRAVDLPFLMPIE
                     DIFTVPGRGTVVTGRIERGILKVNDPVSIVGIRPTRDTVCTGVEMFRKLLDQGEAGDN
                     IGALLRGVDRKDVVRGQVLAKPKSISPYSKFVGTVYVLSKEEGGRHSPFFSGYRPQFY
                     FRTTDITGTVNLPEGKQMVLPGDHTDIHVELINPIAMDKGLRFAIREGGRTVASGQVT
                     EITA"
     gene            complement(23815..24285)
                     /gene="rpsG"
                     /locus_tag="PLF67_01750"
     CDS             complement(23815..24285)
                     /gene="rpsG"
                     /locus_tag="PLF67_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOE88770.1"
                     /translation="MSRRSTAPVREVLPDPVYGSTVVEKFIRRMMVDGKKSISTRILY
                     DAMKTLGEKTNEPPLEVFLKALENVKPVVEVKSRRVGGATYQVPVEVRENRREALAMR
                     WLIAAARNRSGKSMAEKLGAELLDAYQNTGTAFKKKEDTHRMAEANKAFSHFRW"
     gene            complement(24297..24671)
                     /gene="rpsL"
                     /locus_tag="PLF67_01755"
     CDS             complement(24297..24671)
                     /gene="rpsL"
                     /locus_tag="PLF67_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOE88771.1"
                     /translation="MPTINQLVRKGRKQTVHKTKSPALEGCPQKRGVCTRVMTVTPKK
                     PNSALRKVARVRLSNGIEVTAYIPGIGHNLQEHSVVLLRGGRVKDLPGVRYHIIRGAK
                     DTLGVNDRRRSRSKYGTKRPKA"
     gene            complement(24811..29091)
                     /gene="rpoC"
                     /locus_tag="PLF67_01760"
     CDS             complement(24811..29091)
                     /gene="rpoC"
                     /locus_tag="PLF67_01760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOE88772.1"
                     /translation="MREIQEFDSIMIKLASPEQIKGWSYGEVKKPETINYRTLRPERD
                     GLFCERIFGTTKEWECHCGKFKSIRYKGVICDRCNVEVTNTKVRRERMGHITLAAPVS
                     HIWYYRSVPSRMSMLLDITRNALQSILYYEKYVVINSGDTTLKPKQLLTEEEYWDARE
                     QFGDSFEAGMGAEAVKKLLTNLNLEELSRELREQMRQKADKADKRLLKRIEVCENFRD
                     SGNRPEWMILTVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLDRLVKLNA
                     PDIIIRNEKRMLQEAVDALFDNSKRKRVVKGASNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELRMHQCGLPSKMALELYKPFIMKKLVQDGVVYNIKKAKSLVEE
                     ETDAVWAILDEVVKEHPVLLNRAPTLHRLGIQAFDPVLVDGKAIKLHPLVCHAYNADF
                     DGDQMAVHVPLTHAAQMESWTLMLSVTNLLDPANGKPIVYPSQDMVLGINYLTRELEG
                     APGENKYYDSIGEIENAIDSGLLSYNARIRYKLESGEKIETTPGRVLFNAVLPSSVPF
                     QNMNLGDKELRTLIGDTLKANKNSIAVEMLDAIKDIGYKYATLFGATIGLSDMLVPQA
                     KEALMEKASQEQQRIMEQYRQGHITQEERYNRVIEVWTQTNEQLTDELMEELRKDQQG
                     FNPLFLMADSGARGSKTQIRQLGGMRGLMARPSGDVIEFPIKSNFKEGLSIIEFFIST
                     NGARKGLSDTALKTAEAGYLTRRLVDISQDVVVNEDDCNTINGIWRGAIKDGDEIVES
                     ISERIVGRVPVEDILHPFSREVLAKANVEIDEATALAIEESGLESVLLRTVLTCEAKH
                     GVCAKCYGRNLATNRPVVIGEAVGIIAAQSIGQPGTQLTMRTFHVGGTASTSAEENKL
                     TFNYPVIIGTISGARVVRESDQINVFTRKGTIEYFRANAIIEEGSYDTLLVEDGSTVA
                     KGTPIYVKKGKNVLSKENGSVRIVDGRVFLLGHSTLHTVKAGSELLVDTGAFVEAKRT
                     IVTFDPFSEPILAEESGFASFVDIKLGTTLIEEVNEETGNIEKKITEHSLESLQPRIE
                     ITSEKKGKGDVLAVYLLPGGSYIQVADNAKVTKGMILAKLLKEGIKTKDITGGLPRVG
                     ELFEARRPRNAAILAKVSGLVSFGNIVKGKRTIAVTDSYGHEFKHMVPMGRNLLVRDG
                     DNVEAAEALCDGQIDPHDILEILGENALQSFLVDEVQEVYRMQGVNINDKHLGVIVRQ
                     MLRKVEIAHVGDTNLIHGQQVDKFRFFEENDRVIAEGGEPAVARPLLLGITRASLSID
                     SFISAASFQETTKVLTNAAIAGSKDELRGLKENVIIGQLIPAGTGMRRYRDIKLQDEE
                     LLALQQKVDAVKESRRLEMIEENDELDLEDEVIQSVGDYADEYGDDEE"
     gene            complement(29103..32615)
                     /gene="rpoB"
                     /locus_tag="PLF67_01765"
     CDS             complement(29103..32615)
                     /gene="rpoB"
                     /locus_tag="PLF67_01765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOE88773.1"
                     /translation="MVANGKSITRTYIGTESREVCELPNLIGVQLESYERFLQLETIR
                     KGLEPDPDLGLENVFRSTFPIDSPNGEMRLTYRGYTIDYDNVKFTETECKKKGRSYSV
                     PIKVTISLEFFATGEMREKEIFFGDIPLMTDRGTFIINGAERVVVSQIHRSPGVIFSH
                     DKDVYSSRIIPYRGSWLEFEIDEKKSLIYAKIDRKKKILGTLLLRAIGYDSREKIIDA
                     FYKSEDVELIDDPEMRTTLENRYLAKDVYAMINGEERKVLRAGDLLHAHEIDELFKLG
                     VNLIQLVDLKGEGSLHSEIILNCFEIEDAKYTREGFDEPTKEDVLTPIFSVLMPGEMI
                     AIERAEKDLPDMFFSPRKYDLGSVGRYKFNKKFGEELEDNDAVADESTVLTPEDITNT
                     MGYLIKVFIHEANVDDIDHLGNRRVRSVGELLQNALRGAFARMERIAKERMNLETGSV
                     KPQDLISIKPIVAAIKEFFGSSQLSQFMDQVNPLAELTHKRRLNALGPGGLSRDRAGF
                     EVRDVHYTHYGRICPIETPEGPNIGLIVSLANYTRINRHGFLETPYRRVVDGKVTDQY
                     RFLDANDEEKYYIAQASAAFDEDGQFLDKEVPVRHSGDYSTRAPEEIRYMDISPKQVI
                     SVAASLIPFLEHDDANRALMGSNMQRQAVPLVFPETPRVGTGMEGKTAYDSGVLVKAH
                     RSGVVSYVSSDKIIVIPTSSEIEGEMDVYEVQKYERTNQDTCFNQKPIVEVGQRVEVD
                     QVLADGPATQHGELALGRNILVGFVPWHGYNYEDAVLISERVVKDDIFTSVHIKEFTT
                     DIRETKLGPEKLTRDIPNTSEKMLEQLDADGIVRIGATVRPGSILVGKVTPKSESDTT
                     PEFKLLNSIFGEKAKEVRDTSLKVPHGTEGTIIDIQRLKRSNNDELPPGVEETVKVLI
                     ATKRKLRQGDKMAGRHGNKGVVSKILPEEDMPFMEDGTPLDVCLNPLGVPSRMNIGQL
                     METQLGWAAVALDEWYSTPVFQSASMEQLEEKMREAGLPEDSKAILYDGQTGVPFVNP
                     VFCGYIYYLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYG
                     AANTLQELLTIKSDDMNGRVKIYESIVKGEPATTAGMPEAFNVLVQEIRGLALDMSVY
                     DADGNLVPLTERDEELLAKQGSAN"
     gene            complement(32762..33151)
                     /gene="rplL"
                     /locus_tag="PLF67_01770"
     CDS             complement(32762..33151)
                     /gene="rplL"
                     /locus_tag="PLF67_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOE88774.1"
                     /translation="MATIKKEEILEAIANMTVMEIAELIEMMEEKFGVQAAAAVAAGP
                     AAGAEVEAVEEPTEFNVILKAADPAKKIAAIKEVRAITGLGLKEAKDVVEAGGATIKE
                     SVSKADADALKEKLEAAGCTVEIVPVD"
     gene            complement(33219..33746)
                     /gene="rplJ"
                     /locus_tag="PLF67_01775"
     CDS             complement(33219..33746)
                     /gene="rplJ"
                     /locus_tag="PLF67_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOE88775.1"
                     /translation="MDYKTRITPAKEEAVQALMDEFKAYDGFIFADYRGMTVEQITKL
                     RRNLREKDAAFRVVKNRFAKIALTNMDMESDEHLTGPTAIALAKGDGANLVAKDLFNI
                     ARDGAPIKVKGALIDGMRFDAAQIEAFSKLPTRLELISSLMGTMKAPVQKLAATLLAY
                     VKEKGGDAVGAAKEE"
     gene            complement(33759..34442)
                     /gene="rplA"
                     /locus_tag="PLF67_01780"
     CDS             complement(33759..34442)
                     /gene="rplA"
                     /locus_tag="PLF67_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOE88776.1"
                     /translation="MKHGKKYRASAEKVDRTKFYTLDEATALVKEAAFAKFDETVEVH
                     IKLTLKKSQSVRDTVVLPHQFSAQKRVLVFAKGEKADEARAAGATYVGDDDLIEQIRK
                     GWMDFDVAVATPDMMKDVGRLGPILGRRGLMPNPKTQTVTNDITGALAELMKGRVEFR
                     SDRTGVVHLAVGKVSMDPAHVKENTLEVVREVIRKKPADAKGDYLASVTLASTMGPGV
                     RVDVRDLSL"
     gene            complement(34442..34873)
                     /gene="rplK"
                     /locus_tag="PLF67_01785"
     CDS             complement(34442..34873)
                     /gene="rplK"
                     /locus_tag="PLF67_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOE88777.1"
                     /translation="MAKKKVTAIIKLQCPAQKATPAPPVGPALGPHGVSAPQFVQEFN
                     EKTKNYEPGLVLPVVITVYADRTFSFILKTPPAAVLIKRALGIAHGSGTPNKTKVGKL
                     TKDQVMEIAKTKMPDLNANDLEAAMKIVAGTARSMGVEVEK"
     gene            complement(35033..35593)
                     /gene="nusG"
                     /locus_tag="PLF67_01790"
     CDS             complement(35033..35593)
                     /gene="nusG"
                     /locus_tag="PLF67_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOE88778.1"
                     /translation="MAKGWYVVQTYSGYEQKIERIINKMRESDTDFALVCTDVKVPME
                     TVIEIKDGQKREVKRKLLPGYILVELELPEHSWKTWVTKIRRIQGVSGFVTPTDRVKP
                     QPLSKAEEKSLFQKTGDLPAEKVFKPKQTFAVGEQVRIVDGPFESFVGTIEEVNLEKA
                     RMRVSVGIFGRSTPVEVDFLQVEKIP"
     gene            complement(35602..35781)
                     /gene="secE"
                     /locus_tag="PLF67_01795"
     CDS             complement(35602..35781)
                     /gene="secE"
                     /locus_tag="PLF67_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOE88779.1"
                     /translation="MKKIIKYFKESYLELKKVVWPSREAVISSTKVVLVSTILVAVFL
                     GLVDFLLLRGILFIL"
     gene            complement(35820..35893)
                     /locus_tag="PLF67_01800"
     tRNA            complement(35820..35893)
                     /locus_tag="PLF67_01800"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35857..35859),aa:Trp,seq:cca)
     gene            complement(35917..36093)
                     /gene="rpmG"
                     /locus_tag="PLF67_01805"
     CDS             complement(35917..36093)
                     /gene="rpmG"
                     /locus_tag="PLF67_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOE88780.1"
                     /translation="MAKDKKGPVEKIALQCTECKRRNYTTEKNRRNLPNKLEFSKYCP
                     HDRKHTLHRETKIK"
     gene            complement(36139..36211)
                     /locus_tag="PLF67_01810"
     tRNA            complement(36139..36211)
                     /locus_tag="PLF67_01810"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36176..36178),aa:Thr,seq:ggt)
     gene            36376..38889
                     /locus_tag="PLF67_01815"
     CDS             36376..38889
                     /locus_tag="PLF67_01815"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOE88781.1"
                     /translation="MSNREKTRYGHTARDIAQDFAEHLKYSQDADLVHTTDEGRYTAL
                     ALSIRDRIVHRWNQSRKTQRQGDSKRVYYLSLEFLMGRAMSNNIINLGIEEEVRQALA
                     SLGYTYEELEAVESDAGLGNGGLGRLAACFLDSLATLEIPAYGYGIRYNYGIFRQQIK
                     NGWQVEQPDNWLRAGNPWEIVRPDVVYPVGFGGSVQVIREGGKDFFKWTPSETVLGVA
                     HDMPIIGYGANTVNTLRLWSAKSVDEFDFAEFNEGDYTEAVRSKITAENLSQVLYPND
                     TLYMGKELRLKQQYFFVSCSLNDIIRRFKRNGRHDWNAFNEFAAIQMNDTHPSLAVPE
                     LMRLLVDIEGLSWEKAWEITVSSLGYTNHTLMPEALEKWSVPMMGSLLPRHLQIIYEI
                     NYRFIQEAVSHFPLQPQILNQISIIEESNPKHVRMANLAIVGSHSTNGVAALHSQLLR
                     DSMFPHFNTIFPDRFNNKTNGITQRRWLLASNPELSKLITKAIGSGWITDFSRMAKLK
                     KFADDSSFVADFAKIKAANKVRASNFLRTDTSIILNPNTFFDVQVKRIHEYKRQLLNV
                     LNILYIYNDLKRGGEETRDMEPTTFLFGGKAAPGYVNAKLIIKLINNIAQVINADQEV
                     NDRLKVHFLPNYRVTMAEALIPATNLSQQISTAGTEASGTGNMKFMINGALTIGTLDG
                     ANIEIAEEAGAENLFIFGNTEEQIEALRPSYDPHSYIRSNPKIRAIVDLLQSGYFNVN
                     EPGIFDPLYRSLLKEGDRYFLFADLQMYADAHRKARELYILDQTEWNRKAVLNVASGS
                     KFSSDRTISEYAEEIWKIKSLPVKQNGHDDTVLEDARKR"
     gene            39042..39422
                     /locus_tag="PLF67_01820"
     CDS             39042..39422
                     /locus_tag="PLF67_01820"
                     /inference="COORDINATES: protein motif:HMM:NF018697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE88782.1"
                     /translation="MESFWNRVANLLNTSTVVELAQILDVKRSTLSSWIHSDRRPPME
                     VLLKITRLTGVDIHRLERGFSYSLEEDDEVAEHHTSIKRFELIEFINSLDDEELEVAW
                     AFVRLCERRAVKTRTSKPVQQPKG"
     gene            complement(39379..40107)
                     /locus_tag="PLF67_01825"
     CDS             complement(39379..40107)
                     /locus_tag="PLF67_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOE88783.1"
                     /translation="MIATYFNALTIVIGSLIGLLVKSRLKPSFEEIIFTSSGLITLIL
                     GFSMAMATSSYLLMLFALALGGALGYWLKIEDRILALGTRMESVTSRGASDGEQARNF
                     ALGFLNASLLFCSGAMTVLGSIQAGTVGDYRLIMIKSIMDGAMAVIFASVYGPGVIAS
                     ALFVLVYQGFFTLAGGWIEPILGERGIGELGAVGGVLLLMIGFNLLNIRKTKTGNFLP
                     AMILAPIFAILTPSVAALVSRFGF"
     gene            40134..40727
                     /locus_tag="PLF67_01830"
     CDS             40134..40727
                     /locus_tag="PLF67_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE88784.1"
                     /translation="MEGSRTTQNLLLGAGGATRSGGEKLFWIYENRPIIHHSVANSLR
                     ANLPTILVLGYRHAEIRSLIGDLVKDPLLTIVINDNWQAGQGSSTKVGARLLDRKKRF
                     FISLGDMPLITPSHYRFLERFLEPVVRPVVNGRVGHPVLHDPFLIDEILKQPDSFTMR
                     ELLSAYEMKKIAVDDPAYIRDIDTPQEYKTLLGNHYG"
     gene            complement(40707..43274)
                     /gene="clpB"
                     /locus_tag="PLF67_01835"
     CDS             complement(40707..43274)
                     /gene="clpB"
                     /locus_tag="PLF67_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOE88785.1"
                     /translation="MNMDKMTIKLRSAIAEADRLAGLRSNAEVTTEHLVLALLGQEDG
                     ILTSILERVGVSPHIVEERFEKAADRLSKAYGASAKRSISGVLANQLYAATTIAGEFK
                     DEYLSAEHVLLAIIRDNSAASGIFKELGLTNENVMQALQAIRGNTRIDSEDPESRYEA
                     LEKYCKDLTLLARSGKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LARRIAGGDVPESLKNKRLLSLDLGALIAGAKFRGEFEERLKAVVKEVTTSEGAIILF
                     IDELHTIVGAGAAEGSTDASNLIKPALSRGELHAIGATTLDEYRKYIEADKALERRFQ
                     PVYTSEPSVESTIAILRGLQERYEVHHGVRIKDEALVAAAVLSNRYITNRFLPDKAID
                     LIDEAASQLKMEIESQPEQLDQIERKILQLNIEEQALKRESDKASKKRLEKLTEERNT
                     LQSERDVLHTQWEEERASIGRIRESKARLEQLKSEEENAEREGDLAKAAQIKHGEIPA
                     LTREIQTWTEALKRENGNQLLREEVSEDDIAKIVSNWTGIPVAKMKSSEAEKYLRLES
                     ILKEEVIGQEEAIQAVSAAIRRNKAGIGDEKRPLGSFLFAGPTGVGKTLLAKVLAQFL
                     FDDEKALTRIDMSEYMEKFSVSRLIGAPPGYVGYDQGGQLTEVVRRRPYSVILFDEIE
                     KAHPDVFNVLLQLLDDGRLTDGQGRVVDFTNAVIIMTSNLGSQQLLGAATHEEGAALV
                     NEVIHSAFKPEFINRLDEIIVFNRLDSDAVHRIIALQLKTLANRLKGRGYSLIWDEKL
                     EDAIFEAGFDPLFGARPIKRAIQRLVENPLSLKILEGAFHEGDVISLSWDGSAVVIS"
     gene            complement(43299..43892)
                     /locus_tag="PLF67_01840"
     CDS             complement(43299..43892)
                     /locus_tag="PLF67_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE88786.1"
                     /translation="MPKKIDHEARKERILQTALKVFAREGYRDSNLSLIASECNISRP
                     TIYQYFKDKEDIYYYAVKLVTARMFNRYATYAWSSEQPFLKRLTVICTDIMQEAHDHE
                     GELTSLVDVMLQMKREGTDFNAIILRRTAKLSILFKRLLNQGMKEGQIITCDANKVAD
                     HLMLLLEASCFQVAFFDTYSIQTSKELVTCYLEGLQA"
     gene            complement(43999..44415)
                     /locus_tag="PLF67_01845"
     CDS             complement(43999..44415)
                     /locus_tag="PLF67_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE88787.1"
                     /translation="MRYLTRRTYGLSPFDSLFDELLSTWGTTQRRVPAVNITEEENAY
                     VLEAELAGYKQDEVSVQIEKHVLKISSNKETNLEEETEKNLVTERCYRCFERSFRLPE
                     DVDENKISAKFADGLLTLTLPKKPVEKPKPIEVKIK"
     gene            complement(44615..45580)
                     /gene="argF"
                     /locus_tag="PLF67_01850"
     CDS             complement(44615..45580)
                     /gene="argF"
                     /locus_tag="PLF67_01850"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOE88788.1"
                     /translation="MDTLKGRSYLTLRDYTEEEILYLLSLAKDLRSKKRGLAYPSHLK
                     GKLLDGKNIVLLFDKASTRTRCSFEVAAFDEGANVTYLTNSQMGKKESIEDTAKVLGR
                     LYDAIEYRGFGQSIVEDLARHSGVPVYNGLTDDDHPTQVLADVLTAVDHTGKQPHELV
                     FTYVGDGRNNVSNALAIGARQLKMEYRIAAPKALHPTVESEHVRVFEDPYEAVKGCDV
                     IYTDVWVSMGEEAKMSERIGLLKEYQVTMDLLKASGNPDVLFEHCLPAFHDLNTTIGM
                     EVYKEFGLTAMEVTDDVFRSSHSVVFDQAENRMHTIKAIMVATLG"
     gene            complement(45721..46530)
                     /locus_tag="PLF67_01855"
     CDS             complement(45721..46530)
                     /locus_tag="PLF67_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE88789.1"
                     /translation="MKKYLAAIFLIVLGASLLFAAGAKEVQDTKTILVGATPVPHAAF
                     LNLIVDDLAASGYTLKVREFTDYVTPNEALESGELDANFFQHIPYLETFNKDRGFHLV
                     NAGGIHVEPFALYSDRYASLEALPDGATIAIPNDPTNEGRALLLLESAGLLTLDSAAG
                     LEATPLDIVANPKNLRFREIEAASLPRVLKDVDGAIINGNYAIPAGLVATRDGLLVEG
                     ADSPYVNVVAVKAGRENEAGIKALVAALRSEKIKAYVAEHYKNGEVVLVTE"
     gene            complement(46582..47247)
                     /locus_tag="PLF67_01860"
     CDS             complement(46582..47247)
                     /locus_tag="PLF67_01860"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOE88790.1"
                     /translation="MSAKIWLLIWNSTLQTLSMVFFSTLFSLILGLPLGVLLFITGEE
                     EQGGIIPMPLLNSVLSRIVNVLRSFPFIILMILLFPLSRLLIGTSIGTAATIVPLSIA
                     AAPFVARVIETALKEVDPGVVQAARAMGSTNFQIVRKVLIPEAMPSLVSGVALTIISL
                     IGYSAMAGAIGGGGLGDLAIRYGYQRFRGDIMLVAVIIILVLVEIVQVVGNRLSARLL
                     ARR"
     gene            complement(47244..48257)
                     /locus_tag="PLF67_01865"
     CDS             complement(47244..48257)
                     /locus_tag="PLF67_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE88791.1"
                     /translation="MQIRLKNLKRSYGSLHAVNGISLSIPSNLIYGIIGKSGAGKSTL
                     VRLISLLEKPDDGEVYFDELRVDNLAKEELIRRRRRVGMIFQNFNLFSSRNAAQNVAY
                     PLEIIGTPKGEIKARVAELLELVGLGDRGKAPISTLSGGQKQRVAIARALACNPDILF
                     CDEATSALDPQTTHSILALLKEIQRKMGLTIVMITHQMEVVRDVCDRVAVLDDGRVVE
                     EGPVTDIFAKPKSDVTKEFLSHLVGTDAGSEEQMVQWSSKRGAYILRFRGVTTDQPIL
                     SQITRETGIDFNIRAGGVQKVGGEVEIGTLIVDIQGSEEEQESVVARLRELGVHVEKE
                     ERP"
     gene            48376..49176
                     /locus_tag="PLF67_01870"
     CDS             48376..49176
                     /locus_tag="PLF67_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOE88792.1"
                     /translation="MKKGILMSGYCYETHLHTNQASACGLSAGRAYIRPYLDAGYSGI
                     IVTDHFFNGNTNIPHRLPWKERVRRLCAGYEEAKEYGDHLGLSVFFGFEYSYGQDEFL
                     VYGLSPEYLAEHPEMMGWDHRTLYETVDNEGGLVVQAHPFRERGYLSRISLHPHTVHA
                     VEVINGENEEEFDRKARKYAEHYGLPMIAGADIHDVKKIDRIARATIFERKLNSIDDF
                     IREVKAGRFTLEQDAGRMSEPSAQSNLPVYLYDGEGRAKKIRAAETWS"
     gene            complement(49252..50055)
                     /locus_tag="PLF67_01875"
     CDS             complement(49252..50055)
                     /locus_tag="PLF67_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE88793.1"
                     /translation="MKYGYCTGFSTSPYFKLGLDLLDMIRKQGFDYVEFPLMNFSNMK
                     EREFASVLEEVKRHNLTSPVACNFFPGSVKLVGREMDKGRIAAYLDEILPKLERLGIE
                     KIILGSGPARTYGPDQTREQALDQFISVIEEVILPRTKAFGIVVCIEPFERTYCNLIV
                     SVMEGMDLVNAIRDPGFELMIDIYHMLSNEESLDSIRKCFPHIKHVHIAGPDRRVPEL
                     NDNYIYRALKILKELGYDETMSFETELPKSQEHLAQIFKKTKDLMSPTG"
     gene            complement(50156..51700)
                     /locus_tag="PLF67_01880"
     CDS             complement(50156..51700)
                     /locus_tag="PLF67_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOE88794.1"
                     /translation="MDVLRYIGEAFVIALSPAGLLALVLSVFVGLIFGMLPGLTSTMA
                     VALLTGLTYNFSGPLAVLSLLAIYIGAISGGNQSAILLNIPGTPSSAATAMEGYPLAL
                     KGKAGLAIFVATGSSFFGTMISIVCVALFTPLLTKFALKFMSHEMFLLALFGIVICGN
                     LTSGGDPLKGWISGFIGLLMSQVGMDTVYSYRRFTYGNINLAGGISLLPVMIGLFGFP
                     EVVKAFSGAKTTAMQQATKYSIKEGLGILRRNWFNILRSGILGMLMGAIPGVGEDTGG
                     WLSYWSEKKVSKTPELWNHGCIDGVVSAETGNNAAIGGAIIPVLSLAIPGSTSAAVLL
                     AAFWMHGYRPGPLLMQDTPHFLYYVIVFMVLASCLMWFLGIVIAKFSVKVLSVDIKIL
                     MPIVFICCVIGAFVVSNRLFDIKLMFVFGILGIIMSFLKMPGAPFLLGIILGNMADEN
                     LRRALLLTEADISIFFTRPISIFFIVLITYLILTQTPAYMRMQEKREAKKAAARAAKA
                     EMRKKD"
     assembly_gap    51741..51840
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<51841..52094)
                     /locus_tag="PLF67_01885"
     CDS             complement(<51841..52094)
                     /locus_tag="PLF67_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88795.1"
                     /translation="MEQKQTRPQTRYGIDFIAGVVTFAVSIYVMIKSITFWKEDFVDV
                     FYYSSGLMPMMIGICLFIFSALYIRRTVKEHTLKGCLVDI"
     gene            complement(52178..>53104)
                     /locus_tag="PLF67_01890"
     CDS             complement(52178..>53104)
                     /locus_tag="PLF67_01890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOE88796.1"
                     /translation="DITLIVPWGAGGSSDLVGRRVTDEMAKIFGVNVSVVNTPGATGT
                     VGMNNCLLKPHDGYTLIANATPHTHYAMGTADWSPKDWDFMAAYYVPCLIAVRKDSPY
                     KTFNDLYQALKNNPANTIKNSIAGIGSSGYNAVMVLSATDATMGKGLNTPYAGGADAI
                     KALLAGEVDFTSQLSNEMIDLLRSGDLRALCALTAEDLNLEGVAKTIPSIKNFIPELG
                     KSLPIGDAFGLMFPSDVPDATKAILEDAFVKACKTNSVVAFADEKGMVLMGATIAESN
                     KLKDDAAVAIGFTLFDLGVAKRSPADLGYKRP"
BASE COUNT        11020 a        15151 c        13663 g        12970 t
ORIGIN      
        1 ataccgcgat gctcagggcc atcagcccga cacctaccca gaggtcgact cgttttaaat
       61 ctttcatcat cccctccctc ctcccggtga agacgttaca aagatactac aaagatgttg
      121 ctccgaacca aggcggagca acatcctcaa atcacgcgat caatatcgat cgccgaaacc
      181 gccttcccga atgatcttct catagcgagc gtcgtccgct tcgacgatct ttgaatactc
      241 tgctccggat ttaaacactg gcgtcgcacc caattctttc atctttgcaa tgtaggccgg
      301 atccgcgaca cactgcttga agatattctc aagcttcttc aggatttccg gcggagttcc
      361 ggtaggagcg atgacccctc tccactgctc cagaaccagg tcgatgttct gttcacggcc
      421 ggttttggcg ggaatgccgt cgatctgttt gtcgctgaag actgcaagaa ttctcagctg
      481 acccccttgg acgtttgata ttccttcggg agcgatggcc atgacggcat cacactgatg
      541 gctcatcaag ccggtgatcg ccggtcctcc tcctgagaag gggacgtgga catacttggt
      601 tccggtcgct ccttcaaaga gcagcgccgc catatggttc cctccaccgg ttccggcatt
      661 gcccatcgag attccctcgg ggttcttccg ggcatcggcg atgaaatcgt cgagggtctt
      721 ccatttggca tccgcattca ccaccagata gttgtaggat gcaacaacct gacacgcctc
      781 ctcaaactcc gttgccgtga aagcgacttt ggtcatgtgg gtcttgatca agtgggcaac
      841 gttccagtgc atgatcgtat acccgtccgg agtcgcgctc ttgaacgccg tgatcgcagg
      901 cactcccgat gccgcacctt cgtttttgat gaccagcggc tgaccgttcg cgtacttcgg
      961 aaacacctcg gcaagcgccc tgaatacgat atccgcaccg ccaccggctg accagccgat
     1021 gaccgccgta accggcttgc ttgggaaatc gtcaatggtt tccgcttgtg cctgggcaaa
     1081 cagagtaccc gtaacaagaa cgagaatcat caagaatgtc agaattttct tcatctcttt
     1141 cctcctaaaa agttgaatca cgcggcttcc tcagccacgc acctatacgg tcactctaac
     1201 acatcaatcc attttgtcaa cttctattac aaattaattt ttaccccttg caatcccgct
     1261 ttcagtgccc tatcattcat ccatatgcag aacgacgacc tcgaattcat caattgcgcg
     1321 aacgttctca aggcgattgg aacggacttg acccagccct cccgggctca gatcgctaaa
     1381 tatctggggt tgagtcgaac cgccgtctcc atggtcgtca agaaactcat ggccgctcat
     1441 ctcatcagcg agctggagac ggaaaaaagc gggcggggac gacccggaac tcccctggtt
     1501 ctcgatggaa cccattggca tgcgatcggg gcggcataca gctcgaagtt atggacgttt
     1561 tgtgtggtaa acctgaatgg agaacctctt cttacccata aagaatacgt tgaaacgacc
     1621 gatcaggatg aaacggtcaa agcgctcctt cagggattgg ggcatgttga atcaaaagtg
     1681 aacaaaccgc tgctgccggg ctatggaatc gggtcgccgg gcttgatcga ccccaaaagc
     1741 ggaaccgtct tccgggccga cgacatgggt tggacaaaac cacttccact caagcagctg
     1801 gttgaagaag cgacggggaa gaaagcgtac atcatcaatc gctatcgggc gaacggggtt
     1861 gccgagctga ccttcggcgg acatgatttg acgaaaaaca tcatttatct tggagtggga
     1921 accggcattg ccggctccgt ataccttgaa ggaacgcttc tgaactccac gaagtatcgc
     1981 ttcgggcaca tggtgatcga tccccacgga ccgctgtgcg ggtgcggcca gtacggctgc
     2041 cttcaagcga tggcatcgga aaaggcgctc ctcacatacg cgcgtgagag gatgaaagag
     2101 gatttgacat tcctggcagc gtacgccgac caaccaataa cggcaaagct tctcgtcgat
     2161 cttgccgaag ggggggatcc tcacgcacaa tcgtgtctct cctacatcgc atcggcgctg
     2221 gggatcgcca ttctgacgct tgccaatgta atagcccccg atgaaatcgt catcggaggc
     2281 ccgatcgggg atgccagtac cttccttgtg gatgaggttc gaaaagcggc gtacgcccac
     2341 ctgcttgact ggcaagtctc gtcactgaaa atcagcaagg gaagccaagg tgattatggc
     2401 tccgtgttgg gagctgcagc cttccttttg gagcaaaaaa tggaactgct cctcggcgaa
     2461 ggctgcccgg tgatctctta aagcggaagc tcgattttct ttccgctctt ggcgctccga
     2521 tagatcgctt caatcaattc aaccgatcgg cgggcttcaa gcccggagac ttcgacggga
     2581 gaatcctctc tgatcgattt cacaaaggaa gcaaaatttc gtgtatgacc atggtgcccg
     2641 atcgcaagcg gatcggcggc tcctccgccg gtttccgttg aatcaaggaa cgttttgcga
     2701 atctcttcat cacccggctc ctcatcttca aactgccagg cgatgatcga ctcttcctcc
     2761 agaatcgcac ttcccttcgt gccaaggatc tccaaacgtt tcaagaatcc gggataggcg
     2821 ctggttgttc cttcgatgac tcccattgca ccactgttga atcgtaacac cgccactgcg
     2881 gtatcttcaa cttcgattcg ctcgtgggaa agcgtatcgg caaaggcgaa tacttccgaa
     2941 acatcaccga ccagccacaa gagcaagtcg attgcgtgga tcgattggtt catcaacgca
     3001 ccaccgccgt ccaacgccca cgttccccgc catgctgcac tgtcatagta cgcctggctt
     3061 cggtaccatt ttacctgagc gtccgccatc acgatctttc cgaaacgccc cttctcaatc
     3121 gcttctttaa ggatttttga agaatcaaag aaccgggact ggaaaatacc ggcaagcttt
     3181 acattgtgtt ccttgcatgc gttgatgatc tgagtacagc gatccttcgt gatctccagc
     3241 ggcttctcga tgattacgtg cttcttcgct ttggctgcag ccagcgcccc gtcaaggtgg
     3301 gctcccgacg gagtggcgat cgcaacgatg tccagccggt catccgcaag gaacttgtcc
     3361 agatcggtat atgccgtgca tccatgcttc ttcgcaaact cattcgcccg ttcttcccga
     3421 tagtggtaac agtttacgag ttcacagccg tccaactctt ttatcgcccg agcatgaaaa
     3481 tcggcgattg cacccaatcc aatgattcca aatccaagtt ttttcatcgc tacctccgat
     3541 atgtgtacct acacgctacc atcacgggaa tggaaagtca aatttttttg taatatcgta
     3601 ttacaaatta ttttggacgg ttacattgtt cgggtattgc cgtatactga ccttgaccat
     3661 ctcttcgact tggagctcac atggatctgt ttccagccct tttggcaatc atcatctccg
     3721 gctttgccgg cggcattttt tctttcggat acaaagcgat cgttcagctg aaacaacgaa
     3781 ccggtccttt catctttctc tatgggctga cctatacgat attcgcagct gtcggcattc
     3841 tgttgatcgg tggggtttcc gccgatccca tcgtgtggct cttcggggtt tcgcacggtg
     3901 tcatcatgtg tatggcgatc ctgttctata tccgcatcat cggaaagatt cgcctcagca
     3961 tctcctggac gatcattcaa ttctcaatct tcattccgct ggtcaccagc cttctcttct
     4021 ttgagaaaca tcttagtccg ctctcggtca gcggaaccgt gttgatgctg gtcgcgatca
     4081 tcatattcag ttttgggaaa aaagagcact cgtccaagcc ggaggggcct ccgatctctc
     4141 ttgcaagctg gctgctcctc ttcttgtctt cgctcttttc gggaatggcg cttaccattc
     4201 ccatggtgta catgaaagcg gccaccgcaa gtcagcccct ctacctgatc ttcgccagcg
     4261 gcgtcggcac cctcctgtgt tccctgttgt tcatccgaaa agaggattgg aagtacatga
     4321 aaggggattg gaaagtggtc ggcgtccctt cgacgatgag ccttctgcaa ataatgagct
     4381 gggcgtttct catggtcggc ctgctggacc ttgatggcgc cgtcgcctac tccctcaagg
     4441 gtttcatcgg gctgctctgc gtctacaccg tttcattttc gttcttcagg gaaaccgtca
     4501 cccgaattga aaagatcgga atcctcgtct ccttgggagc gattcttctt atgacggtct
     4561 cgctctacac cgtcgctctg tagattaacg aatcaatcag atacgaatcc ggagatagct
     4621 cctgtcatca aacgaggtat atgcgggatt caaccccttg gtggctcgat attccgtata
     4681 caagagagga tccttctgga gtagctgctt gagcttcatc tcactctctt cgagggtcgg
     4741 gcgcaggaag ggatagccgt cactggcaag aacgacttct ttggagtcgc tacccaattg
     4801 gacgacgatg atcgattcat agtggtcgaa gaatccgtca agggtgggat acccgaagat
     4861 tgatgtgccg accatccgat tctgaaaggc cgcctgatga gtcatgaact ccgacaatcg
     4921 gtcttgaatg agctcaggat gacgcaggag gtgttcgcgt gagaacccct gaatcagcgc
     4981 ctcttcaatg agcattgtcc ggttcagctc catcaacgtg tcgatcgctt ttgtttcggt
     5041 gtagtggact ccgtcagcaa gcgcctgaca gtcaccgaga atccatattt cccgacgagc
     5101 gttgctcagc atggcgatat aggcgccggg acgcaattcc gcatcttctt cggctgcttc
     5161 aagaagatcg cgggttccat accaccggcg aatcgaatca ttgagcgcat gaaacgtctc
     5221 atccttcgag gcgtgcgggg caagattctc caaacacgcc agcagaaggt ttttcgctat
     5281 gacgccattc gttcgggttt gtcccgaaag aggttttctc gaggagcatc catcgatgac
     5341 cgctgcaaaa tgctcgttga tgaagagccc gtcctcgttt tcaagatccg gcgacttgcc
     5401 catgcacagc tgctcaatga tcttcatcct aatgcctcgt acagtaacag cgtgtttcgc
     5461 ttcggagagt tcacggtttc caagatgatg ttcgccccgc tgtcagcaag cagtttcaac
     5521 acggtacgaa agtcgaagga gtgttgatcg atgctgtcat gtgagtcgac gaagatacct
     5581 cgtcctcgat cggaagggtt tgaatggacg tgggcggtta tgactttctt cgttgcaaga
     5641 atctctttga tcccttccag ataatcaaac ccatagactt gaccggcgat ccacaaatgg
     5701 ccgacgtcaa ggcacaagaa caggtcatcg ctcgctcttg caagactcac catctctttc
     5761 ggagtttgag ccaggtatgc gacgcggggg ttgagattct ccgcagcgag ctgaagcccc
     5821 gctcctcttg ccatcgcacc cagcctgaca agcgcttctt tcgcctgctc gaaatgctgg
     5881 cgatacacct cgctttccgg gtaatccgcc cggcagatcg aacccttctc aaccccgaca
     5941 tagggggcga agcttggccc tgtcaacagc gtttcgcgct cggcgttgga tgcgggtatt
     6001 tccatatcgg taccgtaccc cggatggaga acaacaatct ttgctccgaa ccgttgtgct
     6061 gttttaaaag tccgcttcaa cgcttcgaag ctttcgtcca gcaccgtttt atcgcgcgat
     6121 ccgaagtttg ggaacagcgg ttccgcggga caacaggcat gaacggagac cactcgatcg
     6181 gcgatgatcg gttccacatc ggaaaggaat tgttcgctca tcttgtagga gagctcccat
     6241 tgaagagagg gaaattgttc ctgatgagct tgaatcgctt ctctttccct gaagccgacg
     6301 agagaaagtc cgagaatccg attcatttgg gttcaccgac tacaacgact ttgacaccat
     6361 gactttcaat aacactgcgc acttcggcat cgattcgatc ggtgatcata taatcgacga
     6421 cgctccagtc acacactttc gccaacgaag gatgctccat tttcgacgag tcagccaaga
     6481 acaccacttt tgcggcgctc tcgatcattg aacgcttcga gtccgcttca atcaggttcg
     6541 tacagaagac cccttgctcg gaaagatacc ccgacccgcc catgaaatac caatcggcat
     6601 gaatctgccg ataaaagtct tttgagaagg cacccatcaa ccccatcgcc ggacgcctca
     6661 ggatcccacc ggccatcatc agatcgacgc tctcaacatc catcagctct ttcatgacca
     6721 gcaggctgct cgtaacgacg gtaacgtggc ggcctgccag atgggaagcg atgtgcatcg
     6781 tggtcgaacc gttgtcgatg atgatcgatt gtccatcttt gacaaaagtg acggcgagac
     6841 tcgctatcgc ttctttttcc ttccggaatt cgtggatcgt gttgctgata tagcgggagc
     6901 ttcccttgtt ctcggcgatg atcgcacccc cgtgggttcg aaccaatgaa ccgagggtcg
     6961 caagctcatc caaatcgttt cgaatcgtaa ctttcgaaac acccattcga tttgccaaat
     7021 cgccgacctg aacggaccca tgctcctcca ggagagccaa tatctcgcgt ttacgcagtg
     7081 tagagttcat aattcatcct tcttaaggta gtttaatagg atttcaggga tttgtcatct
     7141 cttttctttc tttgtctttc ctgtcggaat ctgataaata aaaacaaaga ttcacagcac
     7201 agcctccttc aatggggtga agaagatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnggc aaaagccgtc agcttatcgc caaggatgca attaacgctc ggcatcgtca
     7381 cttccacata cggctcttcg gttctcagga agggatggtc aacctttcag tgcaataatg
     7441 gttctcctca aaaaggatgt ccaataaaat atgaaacgat gtgttcattc ccggcgagtc
     7501 atatataaac ttatagtggg ctttatcaat cttgttccta cctgttcgaa tctgttgttt
     7561 cattgaacga aatggataga tttctccggc ctgttcaagg taggattgca cgtcggtacc
     7621 cggctttaca atgatatcaa tatcggtgga cagtcttctt gatttctcaa gaaggagcaa
     7681 caggctggtt ccacccttga agatgaacgg cataccgacc ttgacaagcg cctcaagtaa
     7741 cgccaaagcg aatatgcttc gctcaaggat gatgctgtct actgtaaagc cacttttaat
     7801 gttttcaata tgctcgcgtg taaaacaatc aggcgttacc atacattacc actgaaccat
     7861 acattcaacc gtcgtcttgg tactaagaaa cccaaatacg tccttcacct tgttcctcct
     7921 gcgcgcatac cgcaacattg cgacctgatc gatacggtat gttgaaaaca tcatctcgat
     7981 tgtcgaggga tattcacctt taaagagcat caggtaccgg tttgcaaaca gatccacaat
     8041 caacttctcc aaaggcacat gatatcgttc accacctgct gagggggcct ctgtgaccag
     8101 acgttcaaca ataaccccgt catctgtgcc atattgcagt atctctttga ctccaggcct
     8161 cagtaatacc cttcccggaa atttctcgat aagcgcggag aacacaaaat caaaaccatc
     8221 cttctccacc tcgagaaaaa tcttattgtg cgccaactga tgattgaaaa actcgttgag
     8281 ccaccgtaac tcccagaccc tgaaagaaag cagcggaaag tgtttctcca tatatcggat
     8341 gacctgcatg gctgcgttgg agtacttccc ggcaaagatc cttttcttcg gttcttcccc
     8401 tgctttcttg tattggttgc gcccgaccct gactaccaga tcggaatcga gcaaagcgcc
     8461 cgtcaagtat ctgagctgtg tctccttgaa ggagggatcg taactccggg ctgccagcag
     8521 gatctgttgt ctggagcata cgccatcggg cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nngaatacta cagcctttgt cagtcttcat atcggaaaag atgctcttcg
     8701 gcgcatcatc ccctctccaa tgccgtattt tcatgcactc gatttgctgg attgcactaa
     8761 ggactaccac ctctttcgct cgaagatccg gcttcttccg gtgacataaa aaccgccatc
     8821 acgtggacag cggctcattg ttggtaactg tggcggtgga ggcttccgat ctctccggtg
     8881 ctcgtcagtc ttttttggcg gctttcttct tggtctcttt tttcaccggc ttttcaacca
     8941 gctcaagaat gaccatctca gcagcgtctc cgagacggtt gcccgtcttc agcactctgg
     9001 tatagcctcc gttgcgctcg gtaaagagcg gggcgatctc tgtgaacagt ttgttgagga
     9061 cggctttgtc ggagatgtct cgggcgatct ggcggcggtt gtgcacgctg tcgttctttg
     9121 cccgggttat catcttctcc gccattctcc gaacttccag agccttggct ttggtggttt
     9181 caatccgttc atatcgaaac agactggtca ccatgttgcg cttcatcgcc ttgcgatgtg
     9241 cggcgtttct gctcagcgcg ttgaacccaa tcctatgctt cattctcttc ttccttgtgt
     9301 tcaggtactt tgatagcagt cttgaggatg ctgtagtcgg tcatgccgag gctgaggttc
     9361 cactccttga gcttctcctt gatctcctga agggatttct ttccgaaatt cctcgtcttg
     9421 gcgatctcat cctcgctctt cttcgtcaga tccccgatcg tgcggatgtt cgcgttcttg
     9481 aggcagttgc tcgagcgaac ggtcaattcc agctcctcga cggaggtgtt gaggatcttt
     9541 cggattcgtt cctcttcctc atcgacttca tcgttgctgg agattagcga ctcatcgaag
     9601 ttgatgaaaa tctggaagta atccttggcg atcttcgccg cttccgccag agcgttctgc
     9661 ggaacgatcg tgccgtcggt gtacacctcg atgatcagct tgtcgtagtc ggagcgatat
     9721 ccgacgcggg tcggctcgat cgaatacttc acccgggtaa cggggctgaa tgtggcatcc
     9781 agcggaatcg ttccgatctc ttcgatgtac ttttcgttga tctcactggg aacatagccc
     9841 cggccgaggt cgatctggac ttccatctca aggttcgcat catccatcat cgtaaagagc
     9901 acgagatcct tgttgatgat ctcaacctga tcacgctcaa gggctgctcc ggtcacgaca
     9961 cccgggccct tgcactcgat gaggaggttg gtgccttcgg catcctcagg catctgaatc
    10021 tggagcttct tcaatgcggc gatgatatca ctgatatcct cgcgcacacc gggaagctgt
    10081 tcatactcac ttgagatgag atgggggacg ccatcctcat tgaagctggt aaatttgata
    10141 gcagtgacgg catacccttg gattgaagaa agaaggaccc ttcgcagcgt attgccgatc
    10201 gtcgttccaa agccccgctc aaacggatag gcgatgaact tgccgtagtt cggctcaacg
    10261 gcgctgtgtt cgaaggtgat ccccttcggc tttttaaagc ccttcagaag gtttttgcgt
    10321 gccatggtta ctccttatcg ggaatacaac tcgacgatca actgctcgtt gatcttctcg
    10381 agctcggtta cttcacttct cctcggaaca gcaaggaagg ttcccttcat cgcatccacg
    10441 tccaagctga gccactgata gactccgctc ttggtgtatt ccttgaggtt ctccttgatg
    10501 atgagcatct tctgagcccg cgggctgatc gagatctcat cacccacctt gacgcggtag
    10561 gaaggaatat cgacgcgctt gccgtttacg aagatgtgtc cgtggttcac gaactgagca
    10621 gcctgataac gacttgaagc gaagtgaagg cggaaaacga cgttgtccaa gcgttgctcc
    10681 aagagttgga tcaagttctc accggtcttt ccgggaattc ttgccgcctc gttgaaggtg
    10741 agcttgaact gcttctcaag catcgcgtac gttctcttga gcttctgctt ctcacgaagc
    10801 tgaagaccgt agttggtcgg cttcttcgat cgggctcggg gatccttgcc gggaaggccg
    10861 gtgcgctttc cactgctgtt cagcgggcac ttgccggaaa ggcagcgatt gcccttgagg
    10921 aaaagcttgg tgccttccgc cctgcagtat ctacatttag gtccggtata tcttgccatc
    10981 tggttctatc tcctcatcac actcttctac tcttgcgcgg tcggcacccg ttgtggggaa
    11041 tcggggtgat gtcgcgaatc gatcgcactt tcaacccgag cacaccaagg gttcgaatgg
    11101 cgctttcacg accgactccg ggacctttga caaatacgtt cacttcctga agtccgctgt
    11161 ccatggcggt ctttgccgct ttctcggcag tggtctgcgc agcatacgga gtggatttct
    11221 ttgctccgcg gaacccgagt ccgccggcac tcgcccacga gacggcgttg ccttgcagat
    11281 cggttacggt gacgatcgtg ttgttgaagg tagcctgaat atagacgttt ccttcgaata
    11341 ccgtcttttt tgtcttacgt tttacagttg ccatctgtga tatgctcctt acttcttctt
    11401 accggcgacg gtcttcttcc tgcccttgcg ggttcgggcg ttcgtcttcg tccgctggcc
    11461 gttgaccggc aagccccttc ggtgtctgag gccgcgatag cagccgatat ccataaggcg
    11521 cttgatattc agggctacct cggttcgaag gcgtccctct actttgtact cttcttcgat
    11581 cacctttcgc agtactgcaa ggtcatcgtt ggaaagggtg ttgatattga tatccggatc
    11641 gatatttgtc ttttcacaaa tctcgatcgc cgaataccta ccgattccgt agatataggt
    11701 cagggcaatt ttcgttgcct tgttcggcaa atctacacct gcaattctcg ccatccatgg
    11761 cctcctgaaa ttctcacagg gttgctaccc tactaatttt gtttctgctt gtgcttgggg
    11821 ttctcgcaga tgatccgaac caccccactc cgtcttacta ccttgcattt atcacaaata
    11881 ggtttgacac tcgatcttac tttcatcttc cagctcctcg tgcttagaaa tgcttcgttt
    11941 tcttcttctg cccgatttta aatccatcgt agtggtgcat cttcatgatg ccctcgatct
    12001 ggcggatcgt atcaagctca actccgacca ggatcagcag cgaagtacct ccgaagagca
    12061 tcgcgaccgt cgaggggaag ttgaagaact tctgtacgag cgtcggaatc aaggcgatga
    12121 acgccaggaa caacgaaccg ggcagaacaa tccggttaag aaccttggtt aagtactcct
    12181 caagcttctc cgcccgaacc ccgggaacgg atccaccgtt ctcccggatc tgcttggcca
    12241 tttcgaccgg gttcatcgac acctgggtgt agaagaacgc gaacgctaca atcaacaggg
    12301 cgtagatgat gagatagggg gtgccctgag gatttaacca attggcgaat gcggcaagcc
    12361 agcggacttc cggccccaag gtcgttgcaa tctgcaaggg gaaggagagc agcgcactgg
    12421 cgaaaatgac cggaatgacg cccgaggggt tcaccttgat ggggatgtac gttgattgcg
    12481 caccgtacat ctttcggccc accacgcgct tggcgtagtt taccggaatc tttcggattc
    12541 cctgctcctc gtacacgacg agggcgacta cgatgaggaa catcaccgca acgatcagca
    12601 cgacgatcgg gttcagcacc ccgcccttta tcgcttcgaa caaagcgttc acggcggtcg
    12661 ggaagcgggc gacgataccg gcaaaaatca agaggctgat accgtttccg attccccact
    12721 gggtgatctt gttaccgatc caaatcagca gcatcgaacc ggtggttacg gtaagcatgg
    12781 caatcagggt aaagcttccc aagcccatcg tcagcacgcc ggggatcgac tgtgcgtaaa
    12841 tcgtcactac atatgattga agcagacaca ccactacggt tccatagcgg gtgtactgct
    12901 gcaacttctt gtgccccgaa ggatcggagg cgagcttctt caacgaggga atcaccagca
    12961 tcaaaagttg cagaatgatc tgcgtactga tgtacggcat aaccccgagc atgaagagcg
    13021 agaagttgga gaacgctccg ccgctgaaga agttcaaata ctcggtcagg ccgatcgtcg
    13081 aactggaact ctgggcaagg aagaacagct tcaacacttg cggatcgatt ccgggaatcg
    13141 gaataacggc cccgattctg ctgacaacca gcaatccgag ggttatgaaa atcttcttgc
    13201 gaagactttt gattctatac atctcaacta aggtgtttgc catgacgccc ttctttatct
    13261 gatctggccg cccgcagcct taatcgcttc gattgcgctt gcggaaacct tcaatccttc
    13321 aatgataacc ttcttggtaa gctccccgtt gttgaggatc ttcgcctgag tcttcacgcc
    13381 cttcaccact ccaagctcct tcagagccgc gagggtgacc acatctccat cattgaacac
    13441 tcgggagata tcgtctaagg aaacgacctc atacaccacc ttgaacggct cgttggagaa
    13501 acctcgacga gcgacgcggc gatagagcgg catctgtccg ccctcgaagc ctgggcgcgt
    13561 gcctccgccg gagcgggagt tctgcccgtc gtgtcccctt ccgctggttc gacccttgga
    13621 agaagctccc cgaccgagaa tcgttttctt ggtattggca cccttcggtg cgataatctt
    13681 tgccatctta catctcctcg acttttacga ggtgcgaaac gacgttcacc attcccagga
    13741 taaccgggtt tgcatcatgc accaccgagc tggaaatgcg ccccaagccg agggccttca
    13801 cggtccgtcg ttgcttggga agcgttcccg caagccctct gacgagggta atcttaatct
    13861 gcttctgctt ttttgccttg gccatctctt acccccacat ctcattcagg gatttgcccc
    13921 ggttcttggc aacctcacgg gcattgaaga ggttctccaa tgcgacgaac gtcgccttta
    13981 cggtattgat atggttcttg gagccaagcg acttgctgag gatgtcgtgg attccggccg
    14041 catcgcagat cgctcgaacc gtaccgccgg cgataactcc ggtaccggga accgccggct
    14101 tcaaaagaac gctggcgcct ttgtactttc cgatgatctc atgcggaatc gtggagttct
    14161 tcatcggaat ttgaatcatc gcgctcttgg ctctttccac cgccttgcgg atcgcctcgg
    14221 tgatgtcgtt cgctttcccg aagccatagc cgactttgcc gttctgatca cctacgacca
    14281 cgaccgcact gaaggagaac cggcgtccgc ccttcacgac ttttgctacg cggttgatct
    14341 tgatcagctt ctctgtcagt ccgtcgctct tttctctatc tctgtctctg tctcttctat
    14401 ctcggttcct gtccacactg tccccctaga acttgattcc ggctttccgg gcgccgtcgg
    14461 cgatcgactt gacgataccg tggtagagat gcccgtttcg gtcgaacacg caggtatcga
    14521 tcttcttctc gagcatccgc tttccaatcg cctcaccaag cttcagagca tcgttgacgt
    14581 tgtttttcaa atccttcagc tcaggttcga ccgttgaagc cgcgacaagg gtaacccccg
    14641 ccacgtcgtc gatcacttgg acatacatgt gggtgttgga acgatacacg ctcatccgcg
    14701 gcttggtacc ggtaccgctg atccgctttc ggatgtggcg ctttcgctgc tgcagttttc
    14761 gttgtttatc gattactctt ttcatcttat ccttccaccc ttatcacttc gcacctgcgg
    14821 tcttgccggc cttgcggcgg atcacctcgt cttcgtagcg aattccttta cccttgtacg
    14881 gctccggttt gcgcaatgag cgcagctccg ccgccgtctg tccgacctgc tgcttgtcga
    14941 tacccgagag aatgatcttc gtcggcgttt cgaccaccgc ggtgattcct tcgggaacca
    15001 caaagtcgat cggctgggcg tagccgaggc ttagcgtaag cacgttgcct ttcaagtccg
    15061 cccggaatcc gacaccgttg atgatcaggg tctttgagaa tcctttggag actccttcga
    15121 ccatattcac gaccaactgg cgatagagac cgtgatacgc tcgggcggct tgggagtcat
    15181 cgactcgctt caccgccacc ttgccctctt caatggcgag agtgacttcc tcccggctct
    15241 tgaaattgag cttgcccttt gggccttcaa cgtgaatcat tccatcgacg actgcgaccg
    15301 taactccctt tggcagggcg atcgggttgt ttccaattct ggacattccg cactccttac
    15361 caaatcgagc agatcagctc gccgccgacc ttgttctcgg tcgccttctt tccggtgatg
    15421 acaccgtggg aagttgagac aacaacgacg ccgtggccgt tgtagatgcg cggcatatcc
    15481 cgatagcccg tatatacacg tcttcccgga gttgaaatcc gctcgagccc gtggagaacc
    15541 ggagcttgtt tctcgtcata cttgaggaaa atacggatgt aggaaatacc gtcctttgtt
    15601 actttcttga agttcttcac gtatccctcg ttcttgagga tttttacgat ctgaagcttc
    15661 atcttggacg tagtcacgtc gaccttttca tgtcgcgcca tgttggcgtt tctgactttg
    15721 gtcagcatat cagctactgg atcacttaca gccatttctc tctcctacca actggatttg
    15781 gtaacaccag gaatctggcc ttcgcttgcc aacttcctaa aacagatcct gcacatgtca
    15841 aactgccgca tatagccgcg aggtctgccg cacacccggc atcggttcac ccggcgggta
    15901 ctgaatttcg gctccctttt ggctttgatg atcatcgact tctttgccat atatccacca
    15961 ccttactttg cgaagggcat gcccagatgc ttcagcagag catagccttc ttcgtcggtc
    16021 ttcgcagtcg taacaatggc gatattcaaa ccactgacac gctcgatctt atcgaaatca
    16081 atttccggga agacaatctg ctcggaaatt ccgagcgaat agttgccgcg gccgtcaaag
    16141 gcgttgggct ttactcccct gaagtccttt acccggggaa gagcgatgct gatcagccgt
    16201 tcgaggaagt accacatgtt gtctccccgc agggttacca tcgcaccgat ctccatcccc
    16261 tcgcggagct tgaagttcgc gatggacttg cgggccttgg tcttcaccac gtgctggccg
    16321 gtaatctgtt ccaattcctt taccgcggaa tcaaggagct ttttattcac gatcgcttca
    16381 ccgacgccga cacttaccac aatcttctca agagcgggga tctgcatctt ggaggtgtag
    16441 ccaaactctt tcaacagctg ggcggataca ctctccttgt attgtctttt cagattaggt
    16501 acaaactttt ccatcagatc acttccccgg tcttcttagc gtagcggact ttcttgccgc
    16561 tctcgtcgaa cttataacca atcttgcttg tctgaccttt tccggtcata tatgcaacgt
    16621 tggagacgtg gatcggagcc tccagctcca cgatgccgcc tttgtcctgg gcggactttt
    16681 tgcgcatcgt cttcttgacc atgtttgcgc cctggacaat gacccgttgc ttttcgtggt
    16741 cgatcttgac gatttttcca ctcttgcctt tgtctttgcc ggcaatgatg agcacggtgt
    16801 cgttcttctt caacttcatg tgcaacactc ctacaacact tccggcgcga gggacacgat
    16861 cttcatgtac ttttcccgca gctcccgagc cacgggtcca aagatacgct tgccgcgcgg
    16921 gttattgttg gcatcgatga tgacgcacgc gttatcatcg aacctgatat aggtaccgtc
    16981 aggtcgtcgg tattcctttt tcgtacggac gatcaccgcc cgcatcactt cacctttctt
    17041 gatcgcaccg ttgggcaggg cgctgcggac gcttacggtg acgacatcac ctacgccggc
    17101 ggtatagcgg tgggtccctc cgaggacctt gatcacctgc accgttttcg ccccgctgtt
    17161 gtcggcaacg ttcatgtaac tctgcatttg taccataacc gtctctcctt cctaccgtgc
    17221 tttctcaaca atctggacga ggcggtggtg cttgtccttg cttaaggggc gagtctcgac
    17281 gatccggacg gtatcgccga tccctgcctc gttgcgttca tcgtgggctt tcaccttttt
    17341 ggtgcgggtc agatatttct tgtacagagg gtgcagcttc cgcgaagaga gctcgacgac
    17401 aatcgtcttc tccatcttgt cgctgaccac cactccggtg taactcttcc ttaccttttg
    17461 catatcggag ctctccctta cttcgcagat ttgcggatcc ccaactcata ctctcgaata
    17521 agggtattcg ttcgggcgat atttcttttg atggtacgca cggccagcgg gttttccacg
    17581 tgtccgagca ctcggcgcat cctcaagtcg aagtactctt tgtgcagctg atcccgcttg
    17641 gcgaccaact cctcaagggt catctcttta taggaatgtt tcattactgc acctcccggc
    17701 gggagacgaa cttcgtcttg atcggaagct tgcttcctgc aagcgcaagc gcttcacgag
    17761 cgagctcttc ggaaactccg cccatctcga acatcaccga cccggtcttc accacggcga
    17821 cccatccttc cgggtttcct ttaccattac ccatgcgggt ttcagcgggc ttcttggtgt
    17881 acggcatatc ggggaaaatc cgaatccata cctttccacc gcgtttgata tgacgggtca
    17941 tagcgacacg agccgcctcg atctggcggt tggtgatcca tttcggctca agagccaaga
    18001 ggccatactc gccaaatgcg attgtattgc cgcgggtggc gatgccgtca atggagccac
    18061 gctgcttctt tctgtattta attctcttag gactcagcat tgcttacaaa ctccttgcct
    18121 caagaccttc attcctgctg ctcctgcgaa caagatcacc ggcttcgtct ttgaccgggc
    18181 gatcgtagat ttctccgttg aacacccata ccttgacgcc gacgacacca aagctggtat
    18241 cggcagttgc aaatccgtag tcgatatcac tgcgaagggt gtgaagagga atccgtcctt
    18301 ccttcatcca ttcgctgcga gcgatctcag cgccgccgat tcggccggaa aggcgaatct
    18361 tgaccccttg ggctccgctt tgcatcgcct tggagaccgc gatcttcatc gcccggcgga
    18421 acgatccgcg ggcgacgagc tggcgcgcaa cgttcattgc gatcagctgg gcatccgctt
    18481 caggcttctt cacttccttg atcttgatct gaatcttctt gtcggtgagc ttctggaggc
    18541 gctcgccgag cttttcgacg ttcgctccct tgcttccgat gatgacgccc ggccgactgg
    18601 tggtgatcac catcgtgatc cgctggggct ttcgaatgat ttcaagatcg ctgatctccg
    18661 ctccggaggc ctcgggactc gtcaagagcg ccttgcggag cttcaaatct tcgtgcaacg
    18721 tatccgcata ctcgcggggg tccacatacc acttggattt ccaggtcttg ttgatcccaa
    18781 gtctcagacc gattgggttt actttctggc ccatttactt cctcccgctc gctttttcgt
    18841 cgacgctgac ggtgatatgc gacattcgct tcaacaggat gtcccgcctt ccgtggctgc
    18901 gcggccaaac gcgcttcagc cgaggccctt cattgacctg caggtcggtg atcaccagat
    18961 cctcctcgtc gatcttcttg ttgaggtacg tcgcgttcgc tgcggcgctc ttgagaacct
    19021 tgaggatcag gccggcgccc ttctgcggca tcgcctccag aattgaaacg gcgttcacat
    19081 aggactcgtg ccgtaccaga tccgcgacgg ggcggacctt caggggactg atcatcagat
    19141 acttcgcagt ggcggtatat cctttcccgc cttcttcttt cctggccatg cttaccttcc
    19201 tttcttcttg tcggaggcgt ggcctctgaa aatacgggtg ggagcgaatt ctccgagctt
    19261 atgtccgacc aggttctcgg tgatgaacac cggaacccac gtcttgccgt tatagacgga
    19321 gatcgtaaag cccaccatct cggggatgat cgtcgaactt ctggaatagg tcttgatcat
    19381 ctgcttttga tttgccttca gggaatcctg aactcgctta tagagctttt tttcaacaaa
    19441 aggccctttc ttgattgatc tagccacgtt tcactcctac ttccgtctct tgacgatgaa
    19501 cttgccggaa ggcttcttct tcgagcgggt ctttgagcct ttggtcggct tgccccacgg
    19561 ggttaccggg tggcggccgc cgctggtctt tccttcacca ccaccgtgcg ggtggtcgat
    19621 cgggttcatc gcaacaccgc gaaccttggg tcggcgaccc agatggcggc tcttaccggc
    19681 cttgccgagg ttgacgttca tatggtcttc atttccgagc gagccgatcg tagcgtagca
    19741 ctcgccaaac accatccgca tctcgccgga gggcaggcga agggtaacgt agtccccttc
    19801 cttcgctgca accgtcgcgc ttccgccggc ggagcgaacc atctgtccgc ctttgccgag
    19861 gttcagctcg acgttgtgca cttcaagccc gagggggatc gctttcaacg gaagggcgtt
    19921 gccgaccgtg atcggggctt cgggtccgct gagaaccgtc gttcccactt tgaccccttt
    19981 cggagcgagt atatagcgct tctcgccgtc cgcgtagaac accagggcga tgttggcggt
    20041 tcggttcgga tcgtattcga tcgtccggac gacgccgggg acgccgacct tgtctcgttt
    20101 gaaatcaata attcgatacg ctcgtttatg tccgccgccg cggcgtctga cgctgatccg
    20161 gccgaagttg tcgcgacctg ctttcctgga aagcccgacc gtcaacgact tctccggctt
    20221 gttggtggtg atttcactgt aatccagcga gctcttctgc ctgagcgtgg gcgtataggg
    20281 tttatatgtc ttcaaagcca tgttcgtcct gctccttata caccttcgat ggcttggatt
    20341 gaatcaccct tggccaacgt cacgacagcc ttcttccagt taccggtcgt tcccttgatg
    20401 gagccgcccc ggctgcggga atacttcggc ttgcccttta cgatcatcac gttgcacttc
    20461 acgacatcga ctttgaacag tttgctgacc gcatcgctga tcatgtgctt gttcgcgtcc
    20521 atgtgcactc ggaacgtgta cttcttcatt tccgcctcgc gggcgaggtt gctcttttcg
    20581 ctgagcaccg gctcgatgat tatctgatct gctctcattt gctctgtcct tccgcttcac
    20641 cgtagaaggt gttcaggttc ttcgccgcgc cttcgaggac cagaagcttg cgtccataga
    20701 gcagctcttt ggcactgagg ctgttatagg agagcacgta cagataagga atgttcctcg
    20761 ctgcccggcg cagaagctca tcattttctc cgatgatgag aatcgtgcgg ctctcgtcgg
    20821 tgacaaagtt cttgagaatc gcgctcaggt ctcgggtttt gccgctttcg atcgtgaaat
    20881 cttccacgac aaccaaccgc ccatcctgaa caccgagact taagagactc ttcatcgcaa
    20941 ggcgcttcat tttcttgggc aaccggtagg agtaatctct gggcttcggc ccgaagattg
    21001 ttccaccacc cgtccagatc ggacttttct tatcacccgc tcgggcgttt ccggttccct
    21061 tctgtttgta cggcttggcg ttgctgtagt tcacctcgcc ccgcgttttc gtgctggcgg
    21121 ttccgactcg gcggttggcc agctcgttgt tgacggcgta gtagatcgta ccgttgctca
    21181 cttcgcggtt gaacaccgta tcgtccaact cgatcgtcct gagctccttg ccgtcaattg
    21241 aatagacttt cgttttcatt tccctgcccc tacttcttga ccgctttctt cacgatgaca
    21301 acactctggg cgggaccggg taccgcaccc ttaaccaaga gaacctgcaa ctcttcgtcg
    21361 accttgacaa ctttcaggtt ttgcaccgtt gtgcgctcat ttcccatgtg gccgggcatt
    21421 ctcgttcctt tgaaggtcct cgccggggtc gctgacattg cagtaccgcc gataccgcgg
    21481 tggaacttcg aaccgtgact accacgtcct ccaccaaagc catgacgctt cacaccaccg
    21541 gcgaagccct tgcctttgga ggttcccgtt acatccacaa aggagatatc attgaatata
    21601 tccacaccca acacatcgcc taccttcacg tccgcatcaa agtcgcggaa ctctacaagg
    21661 tgctgtttcg gttcacagac atccttgaat tgaccggcat acggtttggt ggttgtactc
    21721 ttcttcttat ctatggagcc aagcaatgca gcgttgtacc catgcttttc ttcgctgcgg
    21781 ttcgctacta cgacgttgcc ctcaattttg atgacggtta ccggtgtgag gatacccttg
    21841 gcatcaaaca cctgcgtcat tccaattttc ttgccgataa gccctagcat ctcttacctc
    21901 aactctctta ctgtttaatc tctacatcca caccagcagg gagctcaagc ttcatgaggg
    21961 catccatgac actggaagta ggttccagaa tgtctattaa acgcttgtgt gtccgcatct
    22021 cgaactgttc gcgcgacgtc ttgttgacaa agggcgatct caggaccgtg tacttgttga
    22081 tacgagtggg aaggggaaca ggtccgctta ctgtggcgcc tgcccggaca acggtctcca
    22141 caatcgcttt ggaactttgt tcaacaagct ctacatcaaa gccccggagc cgtactcgaa
    22201 ttctctcttt agccattatt cctccaaaaa acaggccttg ccaccaccgt aaggcggtgg
    22261 caagacattc aactgttggt tacgcggtaa tctcggttac ctgaccggaa gcaacggtac
    22321 gaccgccttc gcggatcgcg aagcgaagtc ccttgtccat tgcgatcggg ttgatcagct
    22381 cgacgtgaat gtcggtgtga tcgccgggga gaaccatctg cttgccttcc ggaaggttta
    22441 ccgttccggt gatgtcagtg gttcggaagt agaactgggg tcggtagccg ctgaagaacg
    22501 ggctgtgtcg accaccctct tccttgctca ggacgtacac ggtaccgacg aacttggaat
    22561 acgggctgat ggatttcggc ttggcgagaa cctgaccgcg gacaacatcc tttcggtcga
    22621 caccgcgaag cagagcaccg atgttgtctc ccgcctcacc ctgatcgagc agtttgcgga
    22681 acatttcaac tcccgtgcaa acggtgtcgc gggtcgggcg aattccaacg atggaaacag
    22741 gatcgttcac cttcagaatg ccgcgctcga ttcgaccggt aacaacggtt ccccgaccgg
    22801 gaacggtgaa gatgtcttca atcggcatca ggaacggcag atcgacagcg cggtccggaa
    22861 gcgggatgta ctcatccatc gcatcaagca attcgtcgat gcacttcgtc gcttcggggt
    22921 tatcgggatc ggtcaaggca aggtatgccg aaccggcgat gacaggagcg ttctcgccgt
    22981 caaagccttg atcggtgagc agttcgcgca tctcttcttc aacgagcaga atcaaatcgg
    23041 gatcgtcaac ctggtcggtc ttgttgatga atacgaccaa tgaaggaaca ccaacctggc
    23101 gagcaaggag gatgtgctcc ctggtctggg ccatggctcc gtcggttgct gcaacaacga
    23161 tgaccgcacc gtccatctgg gcggcaccgg tgatcatgtt cttgatgtag tctgcgtgtc
    23221 ccggacagtc gacgtgggca tagtgccgtg ccttgctctg atactctacg tgacgggtat
    23281 tgatcgtaat accgcgctcc ttttcctcgg gagcattgtc aatagcatca taggcgagag
    23341 cggtatcacc gaacatccgc gcgcagtgct gggtgatagc tgcggtaagg gtggtcttgc
    23401 cgtggtcaac gtgaccgatg gtccctacgt ttacgtgtgg ctttgtcctc tcaaatttct
    23461 ctttagccat cagttcctcc ttgtgacgct cctaatgaag tcacaaaaaa ataattgtat
    23521 gccataaagg cccaaagcct ttaccatttc cggcgagaca aactgatttt gaggggtgtt
    23581 gatacagaga agaggattcc agaggtcggt tggcgcctgc gaggcccaag cgacgtagca
    23641 atttgtttta aaaaaacaag ctttgttact ggaaccacct cattatcgaa cctcgacaat
    23701 cggtttggcc ctaataatcc gtacgccggg cgtacggaca cctcaagcaa tatatgcgaa
    23761 aggttttttt atgtcaagcg atgccggcct ccggcgaagg ccggcatatc accgctacca
    23821 tcggaagtgg ctgaaggctt tgttcgcctc ggccattcgg tgggtatctt ccttcttctt
    23881 gaacgcggta ccggtattct ggtatgcatc aaggagttcg gcgccgagtt tctccgccat
    23941 cgatttgccg ctgcggtttc gagcggcagc gatcagccag cgcatcgcaa gggcttcccg
    24001 gcgattttct cgaacctcga ccggcacctg gtaggtggcg ccgccgaccc ggcggctctt
    24061 cacctcgacg accggcttaa cgttttcgag cgccttgagg aacacctcga gcggaggctc
    24121 gttggtcttc tcgccgagcg ttttcatcgc atcatagaga atccgcgtgg agatggattt
    24181 ctttccgtca accatcatcc tgcggatgaa cttttcaaca accgtcgatc cgtataccgg
    24241 atccggcaac acttcccgaa ccggggctgt acttcgtctg gacataccct acccccttac
    24301 gcctttggtc tcttcgttcc atacttggag cggcttcggc gccggtcgtt gacaccgaga
    24361 gtgtctttcg caccgcggat gatgtgataa cgaacaccgg gaaggtcttt gacccttccc
    24421 cctcgaagca acacgaccga gtgctcttgg aggttgtgcc cgataccggg gatgtaggct
    24481 gtgacttcaa taccattgga aagacgcaca cgggcgactt ttctcaatgc agagtttggt
    24541 ttcttcgggg taacggtcat caccctcgtg catacgccgc gcttctgggg acaaccttca
    24601 agagccgggg atttggtctt gtggacagtc tgcttccggc cctttcgtac cagctgatta
    24661 atagtaggca tcgtttcgat acactccctt tttattcgtt gacccgccgc ttctcacaca
    24721 acgggggtct gtgccgccca acgggcgaca cttcatatgt tgatccgcat cagcatgtaa
    24781 cctgcgacgc attccggcat atgacgaaac ttactcttca tcatccccgt attcgtcggc
    24841 atagtcacct acggactgta tgacctcatc ttcgagatcg agttcatcat tctcctcgat
    24901 catctccaag cggcgcgact ccttgaccgc atcgactttc tgctgcagag ccaaaagctc
    24961 ttcatcctga agttttatgt cccgatacct gcgcataccc gttccggcag gaatgagttg
    25021 gccgataatg acgttttcct tcaatccgcg caactcatct ttactaccag caatagcggc
    25081 atttgtcaag actttggttg tttcctggaa ggacgcggcg gagatgaagg agtcgatcga
    25141 cagcgaagca cgggtgattc ccaaaagcag cgggcgggct accgccggct cgccgccttc
    25201 ggcgatgacc cgatcgttct cttcgaagaa tcggaacttg tcgacttgct ggccatggat
    25261 cagattcgta tcgccgacgt gagcaatctc caccttgcgc agcatctggc ggacgatcac
    25321 accgaggtgc ttgtcgttga tgttcactcc ctgcattcga tagacttcct gcacttcatc
    25381 caccaggaat gattgcaggg cgttctcacc aaggatttcc agaatatcgt gggggtcgat
    25441 ctgaccgtcg cacagcgctt cggcggcctc gacgttgtcc ccgtcgcgaa ccagaaggtt
    25501 gcggcccatc ggaaccatgt gcttgaactc atgaccgtag gaatcggtga ccgcgatcgt
    25561 gcgcttgccc ttgacgatgt ttccaaagct caccaatccg ctgactttcg ccagaatcgc
    25621 ggcattgcgc gggcggcggg cctcaaacag ttcgccgacc cggggaagac cgccggtgat
    25681 atccttggtt ttgatcccct ctttgaggag cttcgccagg atcatgccct tggtaacttt
    25741 cgcgttgtcg gcgacttgaa tgtaggaacc gccgggaaga aggtagacgg cgagcacatc
    25801 gcccttgccc ttcttctcgc tggtgatctc aatccgcggc tgcaatgact caaggctgtg
    25861 ttcagtgatc tttttctcga tgtttcccgt ctcctcattg acctcttcga tcagcgtggt
    25921 tccgagtttt atatcgacaa acgaggcgaa tccgctctct tcggcgagaa tcggttcgct
    25981 gaacgggtcg aaggtgacga tcgtcctctt ggcctcgacg aacgccccgg tatcgaccag
    26041 cagctcactt cccgctttga cggtatgaag cgtggagtgt ccgagtagga acactctgcc
    26101 atcaacgatt cgaaccgatc cgttctcctt ggagaggaca ttcttgccct tcttcacgta
    26161 gatgggggtt cccttggcga cagtcgagcc gtcttcaacc aaaagcgtat cgtagcttcc
    26221 ctcttcaatg atcgcattgg ctcggaaata ctcgatcgtc cccttgcggg tgaagacgtt
    26281 gatctgatcg ctttcgcgca caactctcgc cccgctgatc gtaccgatga tcaccgggta
    26341 attgaaggtg agcttgtttt cttccgcgct ggtcgaggcc gttccaccaa cgtggaacgt
    26401 tctcatcgtc agctgcgttc ccggctggcc gatcgactgc gcagcgataa ttcctacggc
    26461 ttcaccgatc accaccgggc ggttcgtcgc cagattgcgc ccgtaacact tggcacacac
    26521 cccgtgcttc gcctcgcagg tgagaacggt tcgaaggagc acgctctcca agcccgactc
    26581 ctcgatggca agagcagtcg cttcgtcgat ctcgacgttc gccttggcga gcacttcgcg
    26641 gctgaaggga tgaaggatgt cctcgacggg aacccgtccg acgatccgtt cgctgatcga
    26701 ttcgacgatc tcatccccat ccttgatcgc accgcgccag atgccgttga tggtattgca
    26761 gtcatcctcg ttgactacca catcctggct gatatcaacc agacgccggg tcagatagcc
    26821 cgcttcggcg gtcttcaatg cggtgtcgga gagacccttg cgggctccgt tggtcgagat
    26881 gaagaattcg atgatcgaaa gaccttcctt gaagttcgat ttgatcggga attcgatgac
    26941 gtctccgctg gggcgggcca tcaggccgcg cattccgccg agctggcgga tctgcgtctt
    27001 cgatccccga gcgccgctgt cggccatcag gaagaggggg ttgaacccct gctggtcttt
    27061 cctcagctcc tccatcagct cgtcggtgag ctgctcgttc gtctgggtcc acacctcgat
    27121 gacgcggttg taccgctcct cctgggtgat atgaccctgc cgatactgct ccatgatccg
    27181 ctgctgctcc tggctcgcct tttccatcag cgcttcctta gcttggggaa cgagcatgtc
    27241 ggacaggccg atcgtcgccc cgaacagcgt cgcatacttg tagccgatgt ccttgatcgc
    27301 atcgagcatc tcgacggcga tcgaattctt gttcgccttc agggtgtcgc cgatcagggt
    27361 gcgcagctcc ttgtctccca ggttcatgtt ctggaagggg acgcttgaag gaaggacggc
    27421 gttgaagagc acccgaccgg gggtcgtctc gatcttctcg ccgctttcaa gcttgtagcg
    27481 gattctggcg ttgtacgaaa gcagaccgct gtcgatcgca ttctcgatct cgccgatcga
    27541 atcatagtac ttgttctccc ccggcgcccc ttcgagctcg cgggtaaggt agttgatccc
    27601 caagaccatg tcttgggagg gatagacgat cggctttccg ttcgccgggt cgaggaggtt
    27661 ggtgacgctg agcatcagcg tccagctctc catctgggcg gcatgggtga gcggcacgtg
    27721 gaccgccatc tggtcgccgt caaagtcggc gttgtaggcg tggcacacga gggggtgaag
    27781 cttgatcgcc tttccatcca ccagaaccgg atcgaacgcc tgaatgccga gacggtgaag
    27841 cgtgggcgcc cggttaagaa gaaccgggtg ctccttgacg acctcatcca ggatcgccca
    27901 gaccgcatcc gtctcctctt cgacgaggct cttcgccttc ttgatgttgt agacgacccc
    27961 gtcctggacc agcttcttca taatgaacgg cttgtagagc tcgagggcca tcttcgaagg
    28021 aagaccgcac tggtgcatcc gaagctccgg accgacgacg atgaccgaac ggccggagta
    28081 gtcgacccgc tttccgagga ggttctgccg gaagcgcccc tgctttcctt tgagcatgtc
    28141 cgaaaggctc ttcagcgggc ggttggaggc acccttcacg acccgcttcc gcttggagtt
    28201 gtcaaagagc gcatcaaccg cttcctgaag catccgcttc tcgttccgga tgatgatatc
    28261 cggggcgttg agcttcacga ggcgatcgag gcggttgttc cggttgatca cccttcgata
    28321 gagatcgttc aaatcactgg tggcgaaacg cccgccgtcg agctggacca tcggcctcag
    28381 atccggcgga atcacgggga tcacggtaag gatcatccac tccgggcggt ttccgctgtc
    28441 gcggaagttc tcgcacacct cgatccgctt caagaggcgc ttgtccgcct tgtccgcttt
    28501 ttggcgcatc tgctcgcgca gctcccggct gagctcctcc agattgaggt tcgtcagcag
    28561 cttcttgacc gcttccgcgc ccattcccgc ttcgaaggag tcgccgaact gctcgcgggc
    28621 gtcccaatac tcctcttcgg tgaggagctg cttgggcttg agggtcgtgt cgccgctgtt
    28681 gatgacgaca tatttttcat agtagagaat cgactgcagc gcgttccggg tgatatccag
    28741 cagcatgctc atccgcgagg ggacggagcg atagtaccag atatggctga ccggagcggc
    28801 gagagtgatg tgccccatcc gctcgcgccg gactttcgta ttggtgacct cgacgttgca
    28861 gcggtcacag atcacgccct tgtagcggat cgacttgaac tttccgcaat ggcactccca
    28921 ctccttcgtc gttccgaaga tccgctcgca gaagagaccg tcacgctccg gtctcagcgt
    28981 gcgatagttg atcgtttcag gcttcttgac ctcaccgtac gaccagcctt tgatctgttc
    29041 aggggaggcg agcttgatca tgatggaatc gaattcttga atctctctca tcacttctct
    29101 ccttaattcg ccgagccttg ctttgccagc aattcttcgt cacgttcggt aaggggcacc
    29161 aggttgccgt cggcatcata aaccgacata tccaaagcaa ggccccgaat ctcttgaacc
    29221 agcacgttga atgcctcggg cattcccgct gtggtcgccg gctcgccctt gacgatcgat
    29281 tcgtagatct tcacccgtcc gttcatatcg tcactcttga tcgtgagcag ctcctgaagg
    29341 gtgttcgcag caccatatgc ttcgagggcc cacacttcca tctctccgag gcgctgaccg
    29401 ccgaactggg ctttaccgcc gagcggctgc tgggtcacca gcgagtaggg accggtggac
    29461 cttgcgtgca tcttgtcgtc gacgaggtgg tgcagcttca gatagtagat gtagccgcag
    29521 aagacggggt ttacgaaggg aactccggtc tgcccgtcat agaggatcgc cttcgaatcc
    29581 tcgggaagac ccgcctccct catcttctcc tcaagctgct ccatcgatgc gctttggaac
    29641 accggggtgg aataccactc gtcgagagcg acggccgccc atccgagctg ggtttccatc
    29701 agctgtccga tattcattcg ggacggaaca ccgagggggt tgagacacac atccaaggga
    29761 gttccgtctt ccatgaaggg catgtcctct tcgggaagaa tttttgaaac gacgcccttg
    29821 tttccgtgtc gaccggccat cttgtcgcct tggcgaagct tccgcttcgt ggcgatcagg
    29881 accttcaccg tctcttcgac cccgggggga agctcatcgt tgttgctccg cttgagccgc
    29941 tggatgtcga tgatcgttcc ctcggttccg tgggggacct tcagcgatgt atcccggact
    30001 tccttcgctt tttcaccgaa gatcgagttg agcagcttga actcgggagt cgtgtcgctc
    30061 tcgctcttgg gggtgacttt cccgacgagg atcgaaccgg ggcggaccgt tgcgccgatt
    30121 cggacgattc cgtcggcgtc aagctgctcc agcatcttct cgctcgtatt cggaatgtcg
    30181 cgggtcagct tctccggtcc cagcttcgtc tcacggatat cggtggtgaa ttccttaatg
    30241 tggacgctgg tgaagatgtc gtctttgacg acccgttcac tgatgagcac ggcgtcctcg
    30301 tagttgtacc cgtgccaggg aacaaagccg acaaggatgt tgcgtcccaa ggcgagttcg
    30361 ccgtgctggg tcgccggtcc gtcggcgagc acttgatcca cttcaacgcg ttggccgact
    30421 tcgacgatcg gcttctgatt gaagcaggta tcctgattgg tgcgctcata cttctgcacc
    30481 tcatagacat ccatctcgcc ttcaatctcc gaggaggtcg ggatgacgat gatcttatcg
    30541 ctggacacgt agcttacaac gcccgaccgg tgcgctttga caagcacgcc ggaatcatag
    30601 gcggtttttc cttccattcc cgttccgacc cgcggggtct cggggaacac cagcgggacc
    30661 gcctggcgct gcatgttcga acccatcagc gcacggttgg cgtcgtcgtg ctccaggaac
    30721 ggaatcaggg aagcggcgac gctgatcacc tgcttcgggg agatgtccat ataccggatc
    30781 tcttcgggag cccgggtgga gtagtcgccg ctgtggcgca ccggcacttc cttatcgagg
    30841 aactggccat cctcgtcaaa agcggcgctc gcctgggcga tgtagtattt ttcttcatcg
    30901 ttggcatcga ggaagcggta ctggtcggtg acctttccgt cgacgacccg tcgatagggc
    30961 gtctcaagaa aaccgtggcg gttgattcgg gtatagttcg ccagggagac gatcaagccg
    31021 atgttcggcc cctcgggggt ctcgatcgga cagattctcc catagtgggt gtagtggacg
    31081 tcgcggacct cgaagccggc gcggtcacgt gacaggccgc cgggaccgag ggcgttcagg
    31141 cggcgcttgt gggtcagctc ggcaagaggg ttcacctggt ccatgaactg tgaaagctgg
    31201 gaagagccga agaactcctt gatcgcggcg acgatcggct tgatcgagat caggtcctgc
    31261 ggcttgaccg atccggtttc aaggttcatc cgctcttttg cgattcgctc catccgggcg
    31321 aacgcgccgc gcagggcgtt ctggaggagt tcgccgacgc tccgcacccg gcggtttccg
    31381 aggtggtcga tatcgtcgac gttggcttcg tggatgaaca ctttgatgag gtagcccatc
    31441 gtgttggtga tgtcttcggg ggtgaggacg gtcgactcgt cggcgacggc gtcattatct
    31501 tccagctcct ccccgaattt tttattgaac ttgtaccggc cgacggagcc gagatcatac
    31561 tttcgggggg agaagaacat atcgggcaga tctttttcag cccgctcgat ggcgatcatc
    31621 tcgccgggca tcaggacgct gaaaatcggg gtcaggacat cttccttcgt cggctcatca
    31681 aacccttcgc gggtgtactt cgcatcttca atctcaaaac agttgaggat gatttcacta
    31741 tgaagcgacc cctccccttt caagtctacg agctgaatga gattcacacc gagcttaaag
    31801 agctcgtcga tttcgtgggc atgcaagaga tctcctgctc gaagcacctt ccgctcttcg
    31861 ccgttgatca tcgcatagac atccttggca agataacggt tctccagcgt cgtcctcatt
    31921 tcgggatcgt cgatcaattc gacgtcctca ctcttataga atgcatcgat gatcttttcg
    31981 cggctgtcgt agccgatcgc ccgaagcagc aaggttccga gaatcttctt ctttcggtcg
    32041 atttttgcat agatcaacga tttcttttca tcgatttcaa attccagcca agatccccga
    32101 tatgggatga tgcgggatga ataaacgtcc ttgtcatgac tgaagatcac accgggggag
    32161 cggtggatct ggctgaccac aacccgttcg gccccgttga tgatgaaggt gccgcggtcg
    32221 gtcatcagag ggatgtcgcc aaagaaaatc tctttttcgc gcatctctcc ggtagcaaaa
    32281 aattcaagac tgatcgtgac tttgatggga acgctgtagg agcggccctt ctttttgcac
    32341 tccgtttccg tgaactttac attatcataa tcgatcgtat agccgcgata cgtcaaacgc
    32401 atctcgccgt tggggctgtc gatcgggaat gtggatcgaa atacattctc aagacccaaa
    32461 tccggatcgg gctccaagcc cttgcgaatc gtctccagct gcaaaaaccg ttcataggat
    32521 tccagctgca cccctatgag attgggcagt tcgcacactt cacgtgattc agtaccgatg
    32581 taggtccgag ttatggactt gccgttggca accattgtgt accctccaaa gtcaaatgaa
    32641 caagcgactc aaatcccccc gcgggagaag atgaggacac aacatgccac cgggggattc
    32701 cggtggcgtt gagaattcaa catttcgcac gcttcgcgaa ttgttctcaa ggatcttatc
    32761 cttagtcgac gggaacgatc tcgacggtac atccggccgc ttcgagcttc tccttcagcg
    32821 cgtcggcatc cgccttgctt acgctttcct tgatcgtggc tcctccggct tcgacaacgt
    32881 ccttggcttc cttgaggcca agaccggtga tcgcccggac ttccttgatc gccgctatct
    32941 tcttggcagg atcggctgct ttgagaatga cgttgaactc ggtcggctct tcaaccgctt
    33001 caacttccgc accggctgcc gggcctgctg caacagcggc cgcagcctgg acgccgaact
    33061 tctcttccat catctcgata agctcggcga tttccatcac ggtcatgttc gcgatcgctt
    33121 ccaaaatttc ttctttttta atagtagcca tattatcttc tccttaagat tgtctctcac
    33181 agtcgtagca ggtactgagt gaagactaag actgtggttt attcttcttt ggctgcgccg
    33241 acggcgtcgc cacccttctc tttgacatag gcaagcagcg tagccgccaa cttttgaacc
    33301 ggcgctttca tcgtgcccat cagcgaggaa atcagttcga gcctggtcgg cagcttgctg
    33361 aacgcctcaa tctgagccgc gtcaaagcgc attccgtcga tcaaggcgcc ctttactttg
    33421 atcggagctc cgtcccgggc gatgttgaag agatccttgg cgacaaggtt cgctccatca
    33481 cccttggcga gggcgatcgc cgtgggaccc gtcaggtgct catcgctttc catgtccatg
    33541 ttggtcaaag cgatcttggc gaagcgattc ttcaccacgc ggaacgccgc atctttctcg
    33601 cggaggttcc tgcgcagctt ggtgatctgt tcaaccgtca tcccgcggta atcggcgaag
    33661 atgaaaccat cgtacgcttt gaactcatcc atcaaagcct ggacagcctc ttccttggcg
    33721 ggggtaattc gagttttgta atccatcttc attcctcctt agagtgagag gtcacgtacg
    33781 tcgacgcgaa cacccggacc catcgtcgaa gcgagggtta cgctggccag gtagtcgcct
    33841 tttgcatcgg cgggcttttt gcggatcact tcgcgcacca cttcaagcgt gttctccttg
    33901 acatgggcgg gatccatcga aaccttgccg acggcgaggt ggacaacccc ggttcgatca
    33961 gagcggaact cgactcgacc tttcatcaac tcggcgagag cgccggtgat atcgttggta
    34021 acggtctgag ttttggggtt gggcatcaag ccccggcgac cgaggatcgg cccgagtcgg
    34081 ccgacatcct tcatcatgtc cggagtagca accgccacat caaaatccat ccaacccttg
    34141 cgaatctgtt caatcaggtc atcatcaccg acgtaggtcg ctcccgcagc ccgcgcttca
    34201 tccgctttct cgccttttgc aaagacgaga acacgcttct gagcactgaa ctgatggggc
    34261 aggacgaccg tgtcgcgaac actctgactc ttcttgagag tcagtttgat atgcacctcg
    34321 accgtctcgt cgaacttggc gaacgccgcc tcttttacga gcgcggtggc ctcatccaac
    34381 gtataaaact tcgttcgatc gactttctcg gcggatgcac gatatttctt tccgtgtttc
    34441 attacttctc aacctccaca cccatgctgc gagcggttcc cgcaacgatc ttcatcgctg
    34501 cttcaaggtc gttcgcgttc aagtccggca ttttggtctt ggcgatctcc atcacctgat
    34561 ccttcgtcag tttaccgact ttggtcttgt tgggagtacc gctaccatgt gcgattccca
    34621 atgccctctt gatcaggacg gcagcgggag gagtcttcag gatgaaactg aaggtcctat
    34681 cggcatacac ggtaatgaca acgggaagca caagtccggg ttcgtagttc ttcgtctttt
    34741 cgttgaactc ctggacaaac tgaggggcac tgacgccatg agggccgagc gccggaccga
    34801 ccgggggcgc gggggtggcc ttctgggcag ggcactgcaa tttgatgatt gcagtcacct
    34861 tcttctttgc catgttcctt tctccttaca caagctcacc tgtatggcgt cttgcgctgg
    34921 tattaacgcc tgtcaacgac aggctcccac ctcataacga gggagtttgc atttttacat
    34981 ggacacttgt taaaaatcag aaaactctcg tgtaagatcc tgtccacgaa tcttacggaa
    35041 tcttttctac ttgtaaaaag tccacttcaa ccggagtgga tcggccgaaa attcccaccg
    35101 agacgcgcat tcgcgctttc tcgaggttga cctcctcgat cgttccgacg aaggattcga
    35161 aggggccgtc gacaatcctg acctgttcgc cgacagcgaa cgtctgtttg ggcttgaaca
    35221 ccttttctgc gggaaggtcg ccggtcttct gaaagaggct cttctcctcc gcctttgaaa
    35281 gcggttgggg cttgacccga tcggtaggag taacaaaccc tgaaacaccc tggattcggc
    35341 gaatcttggt aacccacgtc ttccagctgt gctccggcag ctcgagctcg acaagaatat
    35401 atccgggaag aagctttcgc ttcacttcgc gtttctgtcc gtcctttatt tcaatgaccg
    35461 tttccatcgg caccttcaca tcggtgcaga caagggcgaa atccgtatcg gactcgcgca
    35521 tcttgttgat gatccgctca atcttttgct cgtacccgga ataggtctgt actacatacc
    35581 agccttttgc catgctcaac cctataagat gaagagaatt cctctcaaga gaaggaaatc
    35641 caccaaaccc aggaagacag cgacaagaat cgtggaaacg aggacaacct tggtagaaga
    35701 aatcaccgct tcccgggagg gccagacgac cttcttcagt tcaagatatg attccttaaa
    35761 atacttgatg attttcttca tgagacaggc tccttgtatt caaaggatcg agaagcgaac
    35821 aggccaagaa ggattcgaac cctcaacatc cggttttgga gaccggcgct ctaccaattg
    35881 gagctattgg cctattagct tctcgatcgc tataacctat ttaatcttcg tctcgcggtg
    35941 caaggtgtgc ttgcggtcat gcggacaata cttgctgaac tccaatttgt tcgggagatt
    36001 ccgccgattt ttctcggtgg tatagtttct ccgcttgcat tcggtgcact ggagagcaat
    36061 cttctcaaca ggaccttttt tatccttagc catattctgc tcctcgtcgg atccatccac
    36121 tttcggtgat gaaaaaagag cccccgaacg gaatcgaacc gttgacctca accttaccat
    36181 ggttgcgctc tgccgactga gctacaaggg catcgggcgt ataacagcgg cagcgtcatt
    36241 acacgcaaaa gctaagttac caaagaagca tggcttttgt caatatgctt catccttctt
    36301 tatggtggtt gccccgatgg gatcgtttcg gtatagtttc actcattgta tgctattaag
    36361 cagtaaggag ttctcatgtc gaaccgtgaa aaaacaagat acggccatac cgccagggat
    36421 attgctcagg atttcgccga acatcttaaa tatagtcagg atgccgacct cgtccatacc
    36481 accgacgaag gccgctacac cgccttggct ctctcaatcc gcgatcgcat cgtccatcgc
    36541 tggaatcaaa gcagaaaaac ccagcgccaa ggggattcga aacgggtata ctacctctcc
    36601 ctcgaattcc tcatgggtcg ggcgatgagc aacaacatca tcaacctcgg aatcgaagag
    36661 gaggtgcgcc aggccctcgc ctcccttgga tacacctacg aagagcttga ggccgtggaa
    36721 agcgacgccg gcctcggcaa cggcggcttg ggacggctgg ccgcgtgttt cctcgactct
    36781 ctggcgacgc ttgagattcc cgcctatggc tacggaatcc ggtacaacta cggaattttc
    36841 cgccagcaga tcaaaaacgg ctggcaagtc gaacagccgg acaactggct gagagcgggc
    36901 aacccctggg agatcgtccg tcccgatgtc gtctaccccg tcgggttcgg cggatcggtt
    36961 caagtcatcc gcgaaggggg caaggacttc ttcaaatgga ctccctccga aacggtcttg
    37021 ggcgtagcac acgacatgcc gatcatcggc tatggcgcca acacggtgaa caccctgcga
    37081 ctctggtcag cgaaaagtgt cgacgagttc gacttcgccg agttcaacga aggcgactac
    37141 accgaagcgg tgcgctccaa gatcaccgct gaaaacctca gccaagttct ttaccccaac
    37201 gatacgctgt acatggggaa ggagctcagg ctgaagcagc agtacttctt cgtctcctgc
    37261 tcgttgaacg acatcatccg gcgcttcaaa cggaacggcc gccacgattg gaacgcgttc
    37321 aacgagtttg cggcgatcca gatgaacgac acccacccga gccttgcggt tcccgagctg
    37381 atgcgcctgc ttgtcgacat cgagggtctt tcctgggaga aggcgtggga gatcaccgtt
    37441 tccagccttg gctacaccaa ccacaccctg atgcccgagg cgctggagaa gtggagcgtc
    37501 ccgatgatgg gctcgcttct gccccgtcac cttcagatca tctatgagat caactatcgc
    37561 ttcatccagg aagcggtgag ccacttcccg ctccagccgc agattctcaa tcagatctcg
    37621 atcattgagg aatccaatcc gaagcatgtg cggatggcca acctggcgat cgtcggcagc
    37681 cactccacca acggggtcgc agccctccac agccagctgc tccgtgattc gatgttcccc
    37741 cacttcaaca cgatcttccc cgaccggttc aacaacaaga ccaacggaat cacccagcgc
    37801 cgctggttgc tcgcctcgaa tcccgagctg agcaagctga tcaccaaagc gatcggaagc
    37861 ggatggatca ccgacttctc ccggatggcg aagctgaaga agtttgccga cgattcctcg
    37921 ttcgttgccg atttcgccaa gatcaaagcg gcgaacaagg tgcgggcttc caacttcctt
    37981 cgcaccgaca cctcgatcat cctgaacccc aacacgttct tcgacgtcca ggtgaagcga
    38041 atccacgagt acaagcgcca gctgctcaac gtgctgaaca tcctctacat ctacaacgac
    38101 ctcaagcggg gcggtgaaga gaccagggac atggagccca ccaccttcct cttcggcgga
    38161 aaagcggctc cgggatacgt caatgcgaaa ctgatcatca agctgatcaa caacatcgcc
    38221 caggtcatca atgccgacca ggaagtcaac gaccggctta aagttcactt tctgccaaac
    38281 taccgcgtta cgatggccga agcgttgatt cccgcgacca acctgagcca gcagatttca
    38341 acggcgggta ccgaagcgtc gggaacgggc aacatgaagt tcatgatcaa cggagcgttg
    38401 acgatcggca cgctggacgg agcgaacatc gaaatcgccg aagaagcggg agcggaaaac
    38461 ctcttcatct tcggaaacac cgaagagcag atcgaagcgc ttcgcccttc ctacgatccc
    38521 cactcctata tcaggagtaa tcccaagatc cgagcaatcg tcgatttgct gcaaagcggc
    38581 tacttcaacg tcaatgagcc ggggatcttc gatcccctct atcgctcctt actgaaggag
    38641 ggggatcgct acttcctctt cgccgatctg cagatgtacg ccgatgccca tcgaaaagcc
    38701 cgcgagctgt acatcctcga tcagacggaa tggaaccgca aggcagtcct caatgtcgcc
    38761 agcggttcaa agttctcaag cgatcggacg atcagcgaat acgccgagga gatctggaag
    38821 atcaagagct tgccggtaaa gcagaacggg catgatgaca cagtccttga agatgcccga
    38881 aagcgctgaa gaccgtctaa caagtcgcct tctcgtcagg gtgacttgtt ttcgtcaccg
    38941 tcggtttcat taattgattg ccccataaga agaatctcac aaaggatcat atgcctgccg
    39001 ggttttcgtc attcagggca atcgactgct atatttacgg catggaatcg ttctggaacc
    39061 gcgttgcaaa cctgcttaac acctccaccg tcgtagagct tgcccaaatc ctcgatgtga
    39121 agcgctcgac cctctccagc tggatccaca gcgaccgccg gcctcccatg gaggttctgt
    39181 tgaagatcac cagactgacc ggagtggata tccaccggct tgaacggggc ttctcctact
    39241 cgctcgagga ggatgacgag gtcgccgagc atcacacctc gatcaagcgg ttcgaactga
    39301 tcgagttcat caactccctc gatgatgaag agctcgaggt ggcttgggcg ttcgtgcgcc
    39361 tctgtgagcg gcgagcggtt aaaacccgaa cctcgaaacc agtgcagcaa ccgaaggggt
    39421 gaggatcgcg aagatcggtg ccagaatcat cgccggaaga aagttcccgg ttttcgtctt
    39481 gcggatgttc aacaggttga acccgatcat cagcagcagg actcccccga ccgccccgag
    39541 ctccccgatt ccccgctcgc cgaggatcgg ttcgatccac cctccggcaa gggtgaagaa
    39601 cccctgatag acaagcacga aaagcgcgct ggcgatcact cccggtccat agacggaagc
    39661 gaaaatcacc gccatcgccc cgtccatgat cgatttgatc atgatcaggc gatagtcgcc
    39721 gacggttccc gcttgaatcg agccgaggac cgtcatcgcc ccggagcaga acagcagcga
    39781 cgcattcaga aatccgaggg cgaagttcct ggcctgttcg ccgtcggacg cccctcggct
    39841 cgtcacgctc tccatccggg ttccgagggc cagaatccga tcctcaatct tcaaccaata
    39901 ccccaacgct ccgccgaggg caagggcaaa cagcatcaaa agataggatg atgtcgccat
    39961 cgccatggaa aatccgagga tgagggtgat caaaccgctg gaggtgaaga tgatctcttc
    40021 aaaggaaggt ttcagcctgc ttttcacgag cagaccgatc agggagccga tgacgatcgt
    40081 caaggcgttg aaataggtgg ctatcataat gatttggagt atactcagat tgtatggaag
    40141 gctcaagaac gacccaaaac cttcttctgg gcgccggggg cgctacgcgc agcggagggg
    40201 agaagctctt ttggatatat gagaacagac cgatcatcca ccacagtgtc gccaattcgc
    40261 tgagggcgaa tcttcccacc attctggtgc tcggctatcg gcacgccgag attcgctcgc
    40321 tcatcggaga tctcgtcaaa gatcctcttt tgaccattgt catcaatgac aactggcaag
    40381 cgggtcaggg ctcctcgacg aaggtcggag cccgtctcct cgatcgaaaa aagcgatttt
    40441 tcatctcgct tggagacatg cccctcatca ctccgagcca ctaccgtttc ctcgaacggt
    40501 ttttggaacc ggtcgtccga ccggtcgtga acgggagggt cggtcatccc gtcctgcatg
    40561 acccgtttct catcgatgaa atcctgaaac aacccgattc cttcacgatg agggaactgc
    40621 tgagcgccta tgaaatgaaa aaaatagcgg tcgacgaccc ggcatatatc cgggacatcg
    40681 acacaccaca ggagtataag acactcctag gaaatcacta cggctgaacc gtcccacgaa
    40741 aggctgatca catccccttc atggaaagct ccttccagga tcttcaggct caaggggttt
    40801 tcgacaagcc gctggatcgc ccgcttgatc ggacgagctc cgaagagcgg atcgaacccc
    40861 gcttcgaaga tcgcatcttc gagcttttca tcccagatga gcgaatatcc ccgccccttg
    40921 agccggtttg caagcgtttt gagctgcaag gcgatgatcc gatgcacggc atcgctgtcc
    40981 agacggttga agacgatgat ctcatcgagg cggttgatga actccggctt gaacgcggag
    41041 tgaatcacct cgtttaccaa ggccgccccc tcttcatggg tcgccgcccc gagcagctgc
    41101 tggctgccga ggttgctcgt catgatgatc acggcgttgg tgaagtccac cactctgccc
    41161 tgaccgtcgg taagacggcc gtcatccaga agctgaagca gcacgttgaa cacatccggg
    41221 tgggctttct cgatctcgtc gaagaggatg accgaatacg gccgccttcg caccacttcg
    41281 gtaagctggc cgccctgatc ataaccgaca tatcccggcg gggcgccgat caggcgggag
    41341 acggaaaact tctccatata ttcgctcatg tcgattcgcg taagcgcttt ctcgtcatca
    41401 aagaggaact gagccagcac tttggcaagc agcgtctttc ccactccggt gggaccggca
    41461 aaaaggaacg agcccaacgg tcgcttctcg tcgccgattc ccgctttgtt ccggcggatc
    41521 gccgcgctga ccgcctgaat cgcttcctct tggccgatca cctcttcctt gaggatgctt
    41581 tcaagcctga ggtacttttc ggcctcgctg ctcttcatct tggcgacggg aatccccgtc
    41641 cagttggaga cgatcttggc gatgtcgtct tcgctgacct cttcacggag aagctgattt
    41701 ccgttttccc gcttcagcgc ttccgtccac gtctgaatct cccgcgtcaa cgcggggatc
    41761 tcgccgtgct tgatctgcgc cgctttggcg aggtctccct cgcgttcggc gttctcctcc
    41821 tcgctcttca gctgttcgag ccgagccttg ctttcccgga tccgaccgat cgaagcgcgc
    41881 tcctcctccc attgggtgtg caggacgtcc cgctcgcttt gaagagtgtt ccgttcttcg
    41941 gtgagcttct cgaggcgctt cttcgaggcc ttgtcgcttt cccgcttcag cgcttgctct
    42001 tcgatgttca gctggaggat cttccgctcg atctgatcga gctgttccgg ctggctttcg
    42061 atctccatct tcagctggct cgccgcctcg tcgatcaggt cgatcgcctt gtcgggaagg
    42121 aagcggttgg tgatgtagcg attgctcagc accgccgctg cgacaagcgc ttcgtccttg
    42181 atccgcactc cgtggtggac ttcgtagcgc tcctgcaggc cgcgaagaat cgcgatcgtc
    42241 gattcgacgc tcggctcgga ggtgtatacc ggctggaacc ggcgctcgag ggctttgtca
    42301 gcttcgatgt attttcggta ttcatccaag gtcgtcgccc cgatcgcatg gagctccccc
    42361 cggctgaggg cgggcttgat caggttggag gcatcggtcg agccttcggc ggcccccgct
    42421 cccacgatcg tatgcagctc gtcgatgaag aggatgatcg ccccttcgct ggtggtgacc
    42481 tccttgacga ccgccttgag gcgctcttcg aactcgcccc ggaacttcgc cccggcgatc
    42541 agggcgccca gatccaagct gagcaggcgc ttgttcttca gcgactcggg cacgtcgccg
    42601 ccggcgatcc ttcgagccaa accttcaacg atcgccgtct tcccgacccc gggctccccg
    42661 atgaggaccg ggttgttctt cgtcctccgg ctgaggacct gcatcaccct ccggatctcc
    42721 tcatcccgtc cgattaccgg atcgagcttt ccgcttcggg caagcagtgt cagatccttg
    42781 caatacttct cgagcgcttc atatcgactc tccggatctt cgctgtcgat gcgggtattg
    42841 ccgcggatcg cctgaagggc ttgcatcacg ttctcattgg tgaggccaag ttctttgaag
    42901 attccgcttg ctgccgaatt gtcacgaatg atcgccaaaa gaacgtgctc ggcggaaaga
    42961 tattcatcct tgaactcgcc tgcgatcgtc gtagccgcat agagctggtt tgcaagcact
    43021 ccgctgatcg agcgcttggc ggatgcaccg tatgctttgc tgagccgatc ggccgctttc
    43081 tcaaagcgct cttcgacgat atggggagag acgccgaccc gctccaagat cgaagtgagg
    43141 attccatcct cctgacccaa gagcgccaga acgaggtgtt cggtggtaac ctcggcattg
    43201 gagcgcagtc cggcgagccg atccgcttcc gcgatcgccg aacgcagttt gattgtcatt
    43261 ttgtccatgt tcatatcact tgttgacgca gtgatttctt atgcttgcag tccttcaaga
    43321 tagcaggtga cgagctcttt cgaggtctgt atcgaatagg tgtcaaagaa cgccacttgg
    43381 aagcaggatg cctcgaggag cagcatcaga tgatcggcta ctttattcgc gtcacaggtg
    43441 atgatctgtc cctctttcat cccttggttc aagagacgct tgaagaggat cgaaagcttt
    43501 gccgtccgcc tgaggatgat cgcgttgaaa tcagttcctt cgcgcttcat ctgcagcatt
    43561 acgtccacca agctggtcag ctccccttca tggtcgtgcg cttcctgcat gatatcggtg
    43621 cagatgaccg tcagacgctt gaggaagggc tgttcgctcg accaggcata ggttgcatag
    43681 cggttgaaca tccgggcggt gaccagcttg accgcataat agtagatatc ctctttgtct
    43741 ttgaagtatt gatagatcgt aggtcgggag atgttacact cgctggcgat cagggagagg
    43801 ttggaatccc gatacccctc acgcgcgaac actttcagag cggtctgtaa aattcgctcc
    43861 ttccgtgctt cgtgatcgat ttttttgggc atacacactc tcctgttttc tacactatat
    43921 caccatgttg tagaactaaa caagcatttt tggaagcctt gccccttggc aaggcctcca
    43981 tactgcgtgt actccttctt atttaatctt tacttcgatt ggtttcggtt tctcaacggg
    44041 cttcttcgga agggtgagcg tcaagagacc gtcagcgaat ttggcgctga tcttattctc
    44101 atcgacatcc tccggaagtc ggaaggagcg ctcgaagcaa cggtagcagc gctcggtcac
    44161 gaggttcttc tccgtttcct cttcaaggtt ggtctccttg ttggagctga tcttcaacac
    44221 atgtttctcg atttggaccg aaacttcgtc ctgcttgtac ccggccagct ccgcctcgag
    44281 aacataggcg ttctcttctt cggtaatgtt gaccgccggc actcgtcgct gtgtcgttcc
    44341 ccaagtgctc aacagttcat caaagagtga gtcaaagggg gacaaaccat acgttcttct
    44401 tgttaaatat ctcatatgtg tacctcctac atctgtatat ttctatcacc acatatgatg
    44461 caacaagcat gccaaccctt gtattatagc ctgtaggtac aaatatatct ctataagtta
    44521 atatacaaac taaagctcgc tcctgcgctt caagggagcg ggtcaagggg aggcgatccc
    44581 caaggaggac tgcattaatc gggtcattat gaccctatcc aagggtggca accatgatcg
    44641 ccttgatcgt atgcatccga ttctccgcct ggtcgaacac caccgagtgg gaggaacgga
    44701 acacgtcatc cgtcacttcc atcgccgtaa ggccgaactc tttgtagacc tccattccga
    44761 tcgtcgtgtt cagatcgtga aacgccggaa ggcagtgctc gaagagcacg tcggggtttc
    44821 ccgaagcctt taagagatcc atcgtgacct gatactcctt caagagcccg atccgctcgc
    44881 tcatcttggc ttcctcgccc atcgagaccc atacatcggt gtagatgaca tcacagcctt
    44941 tcaccgcttc ataggggtct tcaaaaactc tcacatgctc actctccacc gtgggatgca
    45001 gcgctttcgg agcggcgatc cggtactcca tcttaagctg tcgggcaccg atcgcaaggg
    45061 cgttgctgac gttgttccgg ccatcgccga cataggtgaa gaccagttca tgaggctgct
    45121 tgcccgtgtg gtcgaccgcc gtcagcacgt cggcgagcac ttgggtcgga tgatcatcat
    45181 ccgttaaccc gttgtacacg ggaacaccgc tgtggcgggc aagatcttcg acgatcgatt
    45241 gtccgaagcc gcggtactca atcgcgtcgt agagccggcc gaggaccttc gccgtgtcct
    45301 cgatcgattc cttctttccc atctgactgt ttgtcagata cgtcacgttc gccccctcgt
    45361 caaaggcggc gacctcgaag gagcagcggg tccgggtcga ggctttgtca aagagcagaa
    45421 cgatgttttt gccatccaga agtttcccct tcagatggga gggatatgcc agtcccctct
    45481 tctttgaacg gagatccttt gccagcgaga ggaggtagag aatctcctct tcagtgtaat
    45541 cgcggagggt gaggtaactg cggcctttga gagtatccat agcgcttctc cttccggaga
    45601 gtattgcata ttcatgcaaa tatcgtcaat aaatatacaa gtatttctat ttccatgcat
    45661 gcagatgcat aaagaagacc tccggcaaca caccggaggc cagatcttta ctacgaccgg
    45721 ttattcggtt acaagaacga cttcaccgtt tttgtagtgt tcggcgacgt atgccttgat
    45781 cttttcgctg cggagcgctg cgaccagagc cttgatcccg gcttcgtttt cccggcccgc
    45841 cttcaccgcg acgacgttga cgtagggact gtcggcgcct tcgacgagca acccgtcacg
    45901 agtggccacg agacccgcgg gaatcgcata gtttccgttg atgatcgccc cgtcgacatc
    45961 cttcagcacg cgaggaaggc tcgccgcctc gatttcacgg aagcgcaggt tcttcggatt
    46021 cgccacgata tcaagagggg tcgcttccag ccccgccgcg ctgtcgagcg tcaagagccc
    46081 ggcgctttca agaagcagga gagctcggcc ttcgttggtc gggtcattgg gaatagcgat
    46141 cgtcgcaccg tcgggaagag cttccaagga agcatagcga tcgctgtaga gggcgaacgg
    46201 ttcaacgtgg atgccgccgg cgttcacaag gtgaaagccg cgatccttgt taaaggtctc
    46261 cagatacggg atatgctgga agaagttcgc atcaagctcg ccgctttcaa gcgcttcatt
    46321 gggggtcaca taatcggtga actcccggac tttgagcgtg tatccgcttg ccgccaaatc
    46381 gtcgacgatc aggttcagga acgcggcgtg gggcacgggc gtcgcaccga cgagaatcgt
    46441 cttggtgtcc tgtacctctt ttgcgcccgc tgcgaagaga agcgatgcac ccaaaacaat
    46501 cagaaaaatc gctgccaaat actttttcat actatactcc tatttcccac cagggataat
    46561 tcatgttctt gatattgttt ctcagcgtcg ggccaagagc cgggcgctga gccggttgcc
    46621 cactacctga acgatctcaa cgagaacgag gataatgatt accgcaacca gcatgatgtc
    46681 gccgcggaag cgctgatacc cgtaacggat cgccaaatcc cccaaccctc cgccgccgat
    46741 cgctccggcc atcgccgagt aaccgatcag gctgatgatc gtgagggcga ctccgctgac
    46801 cagggagggc atcgcctcgg gaatcaacac cttccggacg atctggaagt ttgtcgatcc
    46861 cattgcacgg gcggcttgaa cgacgcccgg atccacctcc ttgagcgcgg tctcgatgac
    46921 gcgcgcgacg aacggagctg ccgcgatcga cagcggaacg atcgtagcgg cggttccgat
    46981 actcgttccg atcaacaacc tcgaaagagg gaacaacaga atcatgagaa taatgaaggg
    47041 gaaggagcgc agcacgttca cgattcggct cagcaccgaa ttcaacaaag gcatgggtat
    47101 gatgccgccc tgctcctctt caccggtgat gaagagcagc actcccaagg ggagccccaa
    47161 tatcagggag aacagggtgg agaaaaacac catgctgagg gtctggaggg ttgaattcca
    47221 aattaagagc cagatcttcg cactcatggg cgttcctcct tttcgacatg gactccaagc
    47281 tcgcggagcc ttgcaaccac gctttcctgc tcctcttcgc ttccctggat atcgacgatc
    47341 aacgttccga tttcaacctc tccgccgact ttctgaactc cgcctgcccg gatgttgaaa
    47401 tcgattcccg tctcgcgggt aatctgactg agtatcggtt gatcggtggt aactccgcgg
    47461 aagcgcagga tgtaggctcc ccgcttgctg gaccattgga ccatctgctc ttcactgccc
    47521 gcatccgttc ccaccaagtg ggagaggaac tctttggtga catcgctttt aggtttggcg
    47581 aagatgtccg ttaccggccc ttcttcgacg acccttccgt catcgagaac cgccacgcgg
    47641 tcgcacacat cccgcaccac ttccatctgg tgggtgatca tcacgatcgt taagcccatc
    47701 ttacgttgaa tctctttcaa caacgcgagg atcgaatggg tggtttgggg atccagggcg
    47761 ctggtcgctt catcgcagaa gaggatgtcg ggattgcacg ccagagcccg ggcgatcgcc
    47821 actcgttgct tctgcccgcc gctgagggtg gagatcggag cttttccgcg atcgccaaga
    47881 ccgacaagtt caagcagctc ggcgacccga gccttgatct cccctttcgg ggtcccgatg
    47941 atctcaaggg gataggctac gttctgggcg gcgtttcggc tggaaaagag gttgaagttc
    48001 tggaaaatca tccccactcg cctgcggcgt cggatgagtt cctccttggc caggttatcg
    48061 accctcagtt catcgaaata cacttcaccg tcatccggtt tttccagaag actgatcagg
    48121 cgcaccaaag tggatttgcc cgctccgctt ttaccgatga ttccgtagat cagattggaa
    48181 gggatggaca gactgatccc gttgaccgca tgaagcgatc cataactcct tttcaagttt
    48241 ttcagacgaa tctgcatata tataccttcc atattcataa aacggattga gaccatcata
    48301 gaggaaaggt ggttcttgaa caagtggagc aaggatatcc accttggaga aggttttgga
    48361 aaaggatata gttggatgaa aaaaggaata cttatgagtg gatactgcta cgagacccat
    48421 cttcatacca atcaggccag cgcatgcggt ctctccgccg gaagagcata tattcggcca
    48481 taccttgatg ccggatacag cggtatcatc gtaaccgacc acttcttcaa cggaaacaca
    48541 aatattcccc ataggctgcc ttggaaagag cgggttcgta ggctgtgcgc gggatatgaa
    48601 gaagccaaag agtacggcga tcatctgggt ctttccgtct tcttcggttt tgaatactca
    48661 tacgggcagg acgagtttct cgtctatggc ctttcgccgg agtatctggc cgagcatcct
    48721 gagatgatgg gttgggatca tcgcaccctc tatgagacgg tcgacaacga aggggggctc
    48781 gtcgttcaag cccacccgtt ccgcgagcgc ggctacttga gcaggatctc ccttcatccc
    48841 cataccgtcc atgccgttga agtgattaac ggggagaatg aagaagagtt cgaccgcaag
    48901 gcccgcaagt acgccgaaca ctacggtctg ccgatgatcg cgggagcgga catccacgac
    48961 gtgaagaaaa tcgatcgtat cgcccgtgcg acgatctttg aacgaaaact gaactccatt
    49021 gatgacttca ttagggaggt gaaagccggc aggtttacat tggaacagga tgccggccgg
    49081 atgagcgagc cgtctgctca atcgaatctt cccgtctatc tttatgatgg agagggacgg
    49141 gcgaaaaaaa tccgggcagc cgagacctgg agctgacagc gaaaaaaggc aagagaccgt
    49201 cggaaatgcg acggcctctt ttcattgaaa gctgagatat tgttggaaac atcaacccgt
    49261 tggtgacatc aggtcctttg tcttcttgaa tatttgggcc agatgctcct ggctctttgg
    49321 aagctccgtc tcgaaggaca tggtttcatc atagcccagc tctttgagaa tcttcaatgc
    49381 gcgatagatg taattgtcgt tcagctccgg cacccttctg tcaggtccgg cgatgtggac
    49441 gtgcttgatg tgcgggaagc atttcctgat cgaatcaagg ctctcctcgt tgctgagcat
    49501 gtggtagatg tcgatcatca gctcgaagcc cgggtccctg atcgcgttga ccagatccat
    49561 cccctccatg actgagacta tcaggttgca gtaggtcctc tcgaaaggct ctatgcagac
    49621 aacgatcccg aaggcttttg tccttggaag gatcacttcc tctatcactg agatgaactg
    49681 atccaaagcc tgttcccttg tctgatcagg cccgtaggtt cttgccggtc cgcttccaag
    49741 gattatcttc tctatcccca gcctctccag ctttggcagg atctcgtcaa ggtaggcggc
    49801 tatgcgcccc ttgtccatct ccctgccgac cagcttcacg cttccgggga agaagttgca
    49861 ggccacaggc gatgtgagat tgtgtctctt gacctcctcg aggaccgagg cgaactccct
    49921 ctccttcatg ttgctgaaat tcatcagcgg gaactcgaca tagtcgaagc cctgcttccg
    49981 gatcatgtcc aagaggtcca accccagctt gaagtatggg cttgtggaga agcccgtaca
    50041 gtaaccgtat ttcatttcag ctccacttga tcccgagctt atcctgatcg tatcctgaat
    50101 atccgggtct cgaaatccta acccggctcg tcagcaaaac ctgttatagc aaacatcagt
    50161 ccttcttcct catttctgcc ttcgcggccc ttgccgcagc cttcttcgcc tccctcttct
    50221 cctgcatcct catgtaggcg ggggtctgcg tgaggatcag ataggttatg agaacgatga
    50281 agaaaatgga gatcggcctt gtgaagaaga tcgagatgtc cgcctctgtc aaaagcagag
    50341 cccttctcag gttctcgtca gccatgttgc ccagaattat tccaagcagg aacggggctc
    50401 cgggcatctt caggaagctc attattattc caagtatccc gaacacgaac atcagcttga
    50461 tgtcgaaaag tctgttcgag acgacgaaag cgcctatgac gcagcagatg aagacaatcg
    50521 gcatcagtat ctttatgtcg accgaaagca ccttgacgct gaacttcgcg atcactattc
    50581 caaggaacca catcagacag gatgcaagaa ccatgaagac gataacgtag tacaggaaat
    50641 gcggcgtgtc ctgcatcaac agcggtccgg gccggtagcc gtgcatccag aaggcggcaa
    50701 gcaggacggc cgccgaagtg gatcccggga ttgccagcga caggaccggt atgatagcac
    50761 ctccgatggc ggcgttgttt cccgtctcgg cggagacgac tccgtctatg cagccgtggt
    50821 tccagagctc gggggtcttg gagaccttct tctcagacca gtatgacagc catccgccgg
    50881 tgtcctctcc tacaccgggt atggcaccca tgagcattcc caagattccg gagcgtagga
    50941 tgttgaacca gttgcgcctg aggattccca gcccctcctt gatgctgtac ttggtggcct
    51001 gctgcatggc agtcgtcttg gctccgctga aggccttgac tacctccggg aagccgaaca
    51061 gaccgatcat gacgggcaga agcgagattc cgcctgcaag gttgatgttt ccgtaagtga
    51121 agcgcctgta ggagtaaacc gtgtccattc ccacctggct catgagaaga cctatgaatc
    51181 cggagatcca tcccttgaga gggtcgccac cgctggtgag gtttccgcag atgacaatcc
    51241 cgaacagggc gagcaggaac atctcgtggc tcatgaactt cagggcgaac ttcgtgagaa
    51301 gcggagtgaa gagagcgacg cagacgatgc tgatcatggt tccgaagaac gaggatccgg
    51361 tcgcgacgaa gatcgccaag cctgccttac ccttcaaggc cagaggatag ccctccattg
    51421 ccgtcgcggc cgaagagggc gtgcccggga tgttcagcag aatggcgctc tggttacctc
    51481 ccgagatggc accgatgtag atagcaagca aagagaggac ggccagaggt ccgctgaagt
    51541 tgtaggtaag gcctgtcaac agggcgacgg ccattgtgga cgtgagaccc ggcagcatgc
    51601 cgaatatcag tccgacgaaa actgaaagca ccaacgcaag caatcctgca ggagagagtg
    51661 cgatgacaaa tgcttcgcca atgtatctta aaacatccat tctcccctcc ttatggcatt
    51721 ggaaccttga acattatctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51841 ttgatgtcaa cgagacaacc cttcagggta tgctccttga ccgttcttct gatgtaaagc
    51901 gccgagaaga tgaagaggca gatgcctatc atcatcggca tgagaccgga ggaatagtag
    51961 aagacgtcca caaagtcctc cttccagaat gtgatgctct tgatcatcac atagatggag
    52021 actgcaaatg tgacgactcc ggctatgaag tctattccat accttgtctg cggtcttgtc
    52081 tgtttctgtt ccatagactt attcctgaat tgataaagca cgaaaataca gcagatggtg
    52141 cggggggtcc ccgcaccatc cgggatttca atctacgtta cggtcgcttg tatccgagat
    52201 ccgcaggtga gcgctttgcg acaccgagat cgaagagagt aaagccgatt gctacagctg
    52261 catcgtcctt gagcttgttg gactccgcga ttgtggctcc catgagaacc atacccttct
    52321 cgtcagcgaa tgcgacgaca ctgttggtct tgcaagcctt gacgaatgca tcctcgagga
    52381 ttgccttcgt tgcatccggg acgtcagacg ggaacatgag tccgaaggcg tctccgatag
    52441 gcagcgactt ccccagctcc gggatgaagt tcttgatcga cgggattgtc ttggctacac
    52501 cctcaaggtt gaggtcctct gccgtgaggg cgcagagggc tctgaggtcg ccggacctga
    52561 gcaggtcgat catctcgttc gagagctgtg atgtgaagtc aacctctccc gcgaggagag
    52621 ccttgattgc gtctgcacca ccggcatacg gggtgttgag gcccttgccc attgttgcat
    52681 ctgtcgctga aagaaccatg actgcgttgt atccggagga tccgataccg gcgatcgagt
    52741 tcttgattgt gttggccggg ttgttcttca gtgcctgata gaggtcgttg aaggtcttgt
    52801 acgggctgtc cttgcgaact gcgatcagac atgggacata gtatgctgcc atgaagtccc
    52861 aatcctttgg tgaccagtcg gcggttccca ttgcatagtg ggtatgcggt gttgcgttcg
    52921 cgatcagtgt gtagccatca tgcggcttga gcagacagtt gttcattccg actgttccgg
    52981 ttgcccctgg cgtgtttacg acgctgacgt tgacgccgaa gatcttcgcc atctcatccg
    53041 tgacacgtct tccgacaagg tctgatgatc cgccggctcc ccatggaacg atcaaggtga
    53101 tgtc
//
LOCUS       DAOSLI010000006        51332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_775_length_51332_cov_26.640775, whole genome shotgun sequence.
ACCESSION   DAOSLI010000006 DAOSLI010000000
VERSION     DAOSLI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 51332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51332
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_775_length_51332_cov_26.640775"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1628)
                     /locus_tag="PLF67_01895"
     CDS             complement(90..1628)
                     /locus_tag="PLF67_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE88797.1"
                     /translation="MKKVLGITLVLVTCVLLGVSAQGAKEQVVKPLEFSVFYSDNATL
                     PFKDTWLTVTEIQKRTGVKVNWEIIPIADYKTKVSLALNTGTNAPDVILYQSTKGENA
                     ALALNGAIVPISDYPEWTPHFNARVKEFGLENDVDLLKLKDGKRYYMPALFDVPFYDG
                     GLILREDLLKKYNLPVPKTFDDLYRVLKEFKRHAPDSYPLTILAGPRVLYRMTMPSWG
                     VSLGKNGASGTNTLSWDYDKKQYFAGAISEEYKSYITFFRKLYAEGLLDPEMAEPIDG
                     DRWTQKLATGKAMASYAYYDQIGGVEANSTIPGFKLQMYPALAGPAGAHHQEKSRTGG
                     GVMFPIKTSRRADFEQLVRAVDEMFFSEENAKLWCLGVEGVTYTVQNGKIVFADEFVN
                     AKEGIYKSMQVKHGTGSDVTQMVWVNAREMTKYDDNYARINAEVEAMGDVIRYIPPTP
                     KFDDKQAEEAGSLQTPLHDAWERWNSAFLTGAKSIDRDWNAYVQEMKNLGIDRFLELY
                     NSNL"
     gene            complement(1706..2200)
                     /locus_tag="PLF67_01900"
     CDS             complement(1706..2200)
                     /locus_tag="PLF67_01900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE88798.1"
                     /translation="MPDMLVKLYELPGSSELLKDLAKEKIRIHRALAPDKERVLNWVG
                     EHSTAGARSEADVCFANQPISLFLATRKREILGFACYNATAKNFFGPTHVLKSMRGKG
                     IGKALLIASLEALKAEGYAYAIIGGVGPAEYYEKTVGATIIEGSTPGIYRDYLGSGLV
                     FDDE"
     gene            complement(2193..3746)
                     /gene="nagZ"
                     /locus_tag="PLF67_01905"
     CDS             complement(2193..3746)
                     /gene="nagZ"
                     /locus_tag="PLF67_01905"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOE88799.1"
                     /translation="MTREELRLMIGQHLVCGFAGTEISEEFRRAVSHHKIANVILFAR
                     NIESKEQLMKLTADIQKLVRSECHTDALICVDQEGGMVTRLSSDFTNIPGAMALSATG
                     KSRNAYDAGFLTATELRSCGVNVDFAPTLDVNSNPDNPVIGVRSFSDDPSVVSEFGVA
                     MARGLQEGGVMACGKHFPGHGDTHIDSHLSLPTVTGDISAHLQPFKAAINNKIEGIMT
                     SHILFPELEDRNLPATMSRTILTDLLKKEMGFEGLVFSDCMEMKAIADNWGTVDGALM
                     ALRAGVDIVCISHHVNLGVEVVELLEKAIQDGTFGMTEFMELKKSTQKILRQKKRLAE
                     VPVKPSSIIGTKEHTRLNRELHEQSITLVNDAPFTLGANPLFIGPRPFRATNVSDDGK
                     QRHFGAALADLMGGDHLLVGDNPTEDEIGEVVKQAKNYQSVVLGTYNGHLHRGQLTLA
                     NALREVTQVCVFALRNPYDLSSLNPSIRSYAAWSYTPMTFEAIARVLKGEIQATGLLP
                     VHLGGRTDA"
     gene            complement(3739..4803)
                     /locus_tag="PLF67_01910"
     CDS             complement(3739..4803)
                     /locus_tag="PLF67_01910"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOE88800.1"
                     /translation="MMKSVLSPLRGKRVALVTNQSSFTPDWKSSRLHLHELIGLSCLY
                     APEHGLGGYVDRGEGVADTTDPLTGLPVRSLYRGEKAQRATFDDLDHLIFDIQDVGVR
                     FYTYISTLRQIMETGIPVTVIDRPNPLGGRVVEGVVLRPEDESFVGPRGLPIRYGLTI
                     GELAYWMNEEYELGCDLTTIDLFPWWRDDLWWNKIVPWVMTSPAIAHQEGLFFYPGMC
                     LLEATNLHEGRGTSAPFELLGAPFIDSNRLADEVRRLDLDGVVFTPISYTPHGGDLVH
                     GLYGHITDVRTFMPVETAVRILSLIVRMYPKQVVITKHFRALAGFDSAELEDPEKVVE
                     RMKAESADFAEATKRYYRYD"
     gene            4851..5780
                     /locus_tag="PLF67_01915"
     CDS             4851..5780
                     /locus_tag="PLF67_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOE88801.1"
                     /translation="MEGIYLGGDGGGSTCRIGAWDRHGTLMTHATGSSSNAYVVGFDE
                     AVRTVRFLIEDIRSRLEGRPILSFCFGNSGLARAEERTRWEAALKEILGNEVPLLLVT
                     DIHLLLAMNDPRRLGLCLVSGTGSVALGRNEHGSEVRSGGMGSALGDEGSGWWIAREA
                     IGRTLRSVEKRDLPTRMGKELLAFFKLPDYSQAISFFNSSERTKSEIARFTLIVAQAA
                     EQGDLLALSIIEEAARELGLLVSSVQKRLEEPYPKRLIISGGTIVHNPLLHELLLKHL
                     DERLEIHLAVEGGLEGARILAQQLITHGSASYE"
     gene            complement(5752..6636)
                     /locus_tag="PLF67_01920"
     CDS             complement(5752..6636)
                     /locus_tag="PLF67_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOE88802.1"
                     /translation="MNAEHLEYEIAGALREINGKYANLSDSERKVADYVIANPKKALH
                     FTVRELAKQSGTSQAAITRFCRHIGFDSFNNFKIRLALDVFDTFDELYTPNLDLESET
                     SPLAVTRTVIAQTQRALGSLEALVSEEVLLACIEALRAAQSIILFGVGASGVVALDLM
                     QKLTRLGMRVFYSAEVDLQLTVASTLTANDLAFIISYSGETEVMVEVANQANKRGCPI
                     IALTMENPNTIQNLADYKLRVPATERIYRQGAGTSRINQLAIIDILYSLLVSQNLDAS
                     IQAIEETMAITHRKQSRG"
     gene            complement(6633..7571)
                     /gene="murQ"
                     /locus_tag="PLF67_01925"
     CDS             complement(6633..7571)
                     /gene="murQ"
                     /locus_tag="PLF67_01925"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOE88803.1"
                     /translation="MKYGIDYALDSLTTEQRNVRTSNIDEMTTLQVLQLINDEDKSVP
                     YVVETQIPRIETLIEDIVAAFRKGGRLFYIGAGTSGRLGVLDASECPPTFGVSPEMVQ
                     GVIAGGREALVRAVENAEDHEEDGIDELIKRGFTKDDVLVGLTASGQAPYVIGAMKWA
                     RSIGAVVGAISCNEHSRTFLHADHRIFLDVGPEVLTGSTRMKAGTAQKLVLNMLTTTS
                     MIRLGKVYGNLMVDLTPVNAKLVERSKRLIQQATGCSPEEAAEAFEESGRRPKRAIVM
                     ILLGIGLDEVMKLEAINNGPIVEMIRTYRKEEKGQE"
     gene            7681..8856
                     /locus_tag="PLF67_01930"
     CDS             7681..8856
                     /locus_tag="PLF67_01930"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOE88804.1"
                     /translation="MKKTSLFIGLMSGTSMDGIDAVLVEITEEDSAIKAVKLLDLASV
                     EYAPELKTLLLGLCSARMMDIDALTRAHFGLGEWNARAVLRLLSKTKVDPKEIAAIGM
                     HGQTIWHAPTKLPFPGITHPFEIGGTLQIGNPQVVSLRTGIPVISDFRAADVALGGEG
                     APLALIIDHLFFSEAGKTVAVQNIGGIGNVTVLPKAGELYAFDTGPGNMIIDALVQRH
                     TGQPYDDGGSLASRGKIDEALLHRLMDDPYFALSPPKSTGREVYGAPYVQRLIEMAEH
                     LPFEDLIATATAFTAWSIADSYKRFVMPRHGLDRVVVSGGGARNKTLLSYLGSYLDRE
                     IVTGEAYGVPDQGREAMAFALLAHESLNRRPSNYPLATGARERTILGTITLQEPVQI"
     gene            complement(8857..10011)
                     /locus_tag="PLF67_01935"
     CDS             complement(8857..10011)
                     /locus_tag="PLF67_01935"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOE88805.1"
                     /translation="MKKRLLVVHGGAPTAVINASLYGVIKEAEGRYGKIYGAKGGSGG
                     ILKEAFVDLLSLPKEAIERLPHTPGSMIGTSRDHIDAASYRRIVDILIANEIDTVLFN
                     GGNGSMDTAGKIAREIQKRNRPIRVIGIPKTIDNDIAVTDHAPGFGSAARYLASSVQE
                     LAADVAALPIHVSVIESMGRNTGWLTAAALIARREGIYGPHLLYAPELAFDEERFLDE
                     VSSLYKKHRHVVVVASEGLRTANGEPIVEPIFQTDRSVYYGDVSAHLAMLVIKRLGVK
                     ARSEKPGILGRSSIAFQSSVDRDEAILVGRKAVQAALEGNSEVMVGFKRISNEPYRVE
                     PVLIPLEEVMLHEKTLPMEYLPSFGTVDESYLSWCKPLIGGPLADFSPVL"
     gene            complement(10008..11159)
                     /gene="nagA"
                     /locus_tag="PLF67_01940"
     CDS             complement(10008..11159)
                     /gene="nagA"
                     /locus_tag="PLF67_01940"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOE88806.1"
                     /translation="MNESLLIRGGKVYRNGRCEELDLFIEDGAFTGFHQNRPAGGVFE
                     AENMHVIPGMIDIHTHGAVGVDFNLFADEQEIEKVVGFFASKGVSGFLPTVLTDEPEV
                     MKTQLSKLARPALLKRFPQILGIHLEGPFLNASYKGAMKEELLKECDLSCFDELQDAA
                     RGMIRVITLSPEVRGCRTLIRELTGRGVRVSLGHSGAGYEETIGAIDAGASSTTHIMN
                     AMKLLHMHDPAILTAVLERDIYAEMIVDGFHLHPPIVRLLLKIKGWDRMISVTDSIMA
                     AGYPDGEYLLGPNRVIVKDGDAKLVENGVRAGSTLTMDKALRNLLDFTRAPLQRIVPL
                     LSTNAARMLGLSDLGEIALGKRADATILDSSMDVVATITSGKIAYRREE"
     gene            complement(11152..12180)
                     /locus_tag="PLF67_01945"
     CDS             complement(11152..12180)
                     /locus_tag="PLF67_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE88807.1"
                     /translation="MARLKDIAKETGLSISTVSRILSNDPNRSFSDEAIAKVQEAAGR
                     LGYFEKYRMRSKALTTMQLSIACILTSDHETYLSPFFAALLEGVQEEVFRQSSRNALT
                     FHTLYVKDPSFPAFLETTNLDGAIILGRTTIDTIQRLKRSIPILLYAGVNRLGNGVDE
                     VICDAESATKEAVEHLLALGHRAIGFIGHIPQKDETINEHRFNGYLGALRKAGIEYDD
                     SLVVNTILTATGGYQATKTLISRGKIPTAIFCSNDTVAMGVLKALDEAGIHVPNDLSL
                     IGFDNIEATAFTKPALTTIDIPKRDLGRLAVKTLLDRIESKRTFPISVRLPFTLIRRE
                     SCKEVADE"
     gene            complement(12168..13436)
                     /locus_tag="PLF67_01950"
     CDS             complement(12168..13436)
                     /locus_tag="PLF67_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88808.1"
                     /translation="MSFEVTISGIKEGAVDSFILYNEGLIQSGLSAEAYAEGPLGWFD
                     PNTSMQSLQAIQSLAARVQARFDLLIVIGIGGSNRGAVAAVGALSPTGTELFFAGDTL
                     SATQLAKAIRLVQERNAALCVIAKDFNTIEPGITFRMLRKELERKYGSSSHERIIAIG
                     SGGEGQLHHLAQRHQWEFLAFPADINGRYSVLSTVGLFPMAVAGVDIENLIAGGIAEH
                     QRLTTLKPLMNPAVRYAAIRNLLSKQGSIIEALVIFEPALEGFARWWVQLFGESEGKK
                     EGALFPTYFSYSEDLHAIGQYVQEGPRIIVETFLNYTYKSSPLVIEPSCEIQDRFDYL
                     DDRPFDTLNEVVYQAALNAHASDHRPVIELLSSQSISAYSLGQSFYFFLFSVYLSSLL
                     LGVNPFTQDGVEGYKKNMYHQLGKGKVWPD"
     gene            complement(13506..14645)
                     /locus_tag="PLF67_01955"
     CDS             complement(13506..14645)
                     /locus_tag="PLF67_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88809.1"
                     /translation="MKKALVSLLVITLAMTMVFAAGAKEEGKKGLVDVRLGIHANEGG
                     TPLAAVAAEKGFFEKYGINPIFTIVESGPAEMTAMRADNRTLDIGYIGAGVAWNPIDG
                     AGNQLSFVYLDCLSNAEMLVAKKGVFKDANGDGKYDFEEIYQGLKGKKVYIEVGTTPG
                     GWFKTLLNLINEGRPVNQQLWISSETASYIAGYKAPNTNAANRVEVVNTLNSNLPAGM
                     ASAAGGMDIVVGFSPATSTIIRTNPNTELVATTVSHFPPSASFPSTWVASNKWMEENP
                     EVAQAFINALYEASVWRAENIDEAMRIGERLSQKPEGTFDPFNLVAPSKKDLQAWFAN
                     KDGAGYHYLKGLYNVAKGNVPAGNKVKSFEEAFNDTFLLKAIATL"
     gene            complement(14688..15488)
                     /locus_tag="PLF67_01960"
     CDS             complement(14688..15488)
                     /locus_tag="PLF67_01960"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE88810.1"
                     /translation="MKKPLIIIKDVRKEFIVEKKPNVVALDGVDLEIEKNEFVCVVGP
                     SGCGKTTLLNIIAGLETFDSGSVTMHGEDIIGPGPERGVIFQQYALFPWMTVRKNIEY
                     GLKFITKADGSRYTKEEKRALAEHYIKMVNLTGFEDSYPKALSGGMKQRVAIARGYAL
                     NSEVLLMDEPFGALDAQTRAQLQEDLLRTWESEKKTIFFITHDVDEAVLLSSRVVIMS
                     ARPGQIKEIVPVDLPYPRTQATKMESEFIKIKNYIWDTVYRMYLEIPK"
     gene            complement(15496..16293)
                     /locus_tag="PLF67_01965"
     CDS             complement(15496..16293)
                     /locus_tag="PLF67_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88811.1"
                     /translation="MAEQKKSARKLKDFSDGSARMRYTLMTVSIIGGFFFWYLITLIP
                     TINTFLASPKQVLRALISESKAGGRYWKDISISLQRVLIGFGLAFVASIPVAFLMGWY
                     PKVRNLIEPWIQFMRTIPPIALIPLVILAMGLGEPPKYTIIFIATFLVMVVTIYQGVK
                     EVDKTLIKAAYTFGAKDRDLFIDIIIPASFPFILVGARLGMASALTTLIAAELTGTIF
                     GLGARIQGAQQFMDTAIVLLGIITIGVIGFILDKILLFIEKNLTKWK"
     gene            16525..17376
                     /locus_tag="PLF67_01970"
     CDS             16525..17376
                     /locus_tag="PLF67_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOE88812.1"
                     /translation="MASTIFLNLKRFDISFDRGGINRLAPSAQWGPTLGSALTGLLSS
                     YSKETRFVAFLPEAHLIGAIQASDADVISFGSQSVHWADTEIGGNFGAFTTERTANAM
                     KDLGCLWTMIGHSEERRDLARLLSLGGATGKELFGALHRILNEKVLAAQKAGLNVLYC
                     IGENADEVNIRTSLLQNQIEAGLAGTDQSLVTLAYEPIWAIGPGKTPPTAEEIASIAK
                     EIKSFTDMELVYGGGLKEENAEAIGSIGSLAGGLVALTRFSGEIGFYPDEFATIVENY
                     TRGRNMS"
     gene            17373..18812
                     /locus_tag="PLF67_01975"
     CDS             17373..18812
                     /locus_tag="PLF67_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOE88813.1"
                     /translation="MKLAFEYGHGLMEANLPDTTDVFIPGETVKDPPVLEDIVGATRA
                     ALENPLGMKPLSRLVTRDSKIVIIFPDKVKGGFQADSHRKVSIPLIIEQCLAAGADER
                     NIKLICSNGLHRKNTDREIRSILGDAVFDRFHPWGQIVNHDSEDWDNLIDLGHEPHYN
                     CPVIMNREVFESDVAILIGHALGNPYGGYSGGYKHCATGLTHWKSIGSHHNPATMHKQ
                     DFVPVNSHSEMRGKFDAIGRYMEERMGKPYFVCDAVLDTNSRQIGVFAGTAEEVQKAS
                     WALADKRTYVPWAKKKYDVMVFGMPQEFHYGNGMGTNPIFILQAISAQIIRHKRVLVD
                     NPVIICSSLCNGYFHDEEFPSYRETFELFQKDYANTLPDIERFGEAFSMRKEYIDAYR
                     YSYGFHPFHAFSMISCGHIAEQHASAIYIVGAMEPGIARAMGMKTRPTFESALSDAVK
                     YVGKEPNILALPRAFKTASVHLCMAEGKE"
     gene            18815..19636
                     /locus_tag="PLF67_01980"
     CDS             18815..19636
                     /locus_tag="PLF67_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOE88814.1"
                     /translation="MIDKAIYTKDKTALLDNPGIPLAVLPTQEETFRSMADDMVDTIR
                     ERNAAGKRTVFIVPVGPVGQYPYFVQRVNEERISLKNVWFFNMDEYLDEHDRPIDFDS
                     HLSFRGFMHREVYQKIRKELVMDTKQRIFPDPDHPRLLTETLERLGGADVCYGGLGIN
                     GHVAFNEPDEISVEEFKELKTRVADIAMDTQIANAIGSLNGALDAMPRRSVTIGFNEI
                     YASRKIRLYCFRDWHRAVVRHAAHGPISTRFPVTILQEHPDCRITMSSNVAEPCW"
     gene            19685..20593
                     /locus_tag="PLF67_01985"
     CDS             19685..20593
                     /locus_tag="PLF67_01985"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOE88815.1"
                     /translation="MRKALCFGSLNIDYVYDVPHILRPGETLASTNRSIYPGGKGLNQ
                     SIALAKAGVATFHAGAVGEGGELLLETLQDSGVDTRFVRTIEGLSGHTVIQRDANGQN
                     NIILYGGSNQAITEEQIDETLQYFSSGDYLLLQNEINLIPSIMTKAAEIGMVIVLNPS
                     PIDEKLLAYPLELVDIFLLNEIEAMDIVGSESPPDELLRSLSSKFPEAQIVLTLGEEG
                     ALYLDRKGGKILKHGIFDVEVVDTTAAGDTFTGYFIATLAKTNDPQEALRVASLASAL
                     VVSRLGAAPSIPTKEEVYSIHIYLKE"
     gene            20653..22206
                     /locus_tag="PLF67_01990"
     CDS             20653..22206
                     /locus_tag="PLF67_01990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE88816.1"
                     /translation="MESTYIYVPGLWLYIVSTVVLLMFMTAQVPRIHLPLSKRIFWVL
                     FTAFLWNFFLILEFMVSTIELKLLYAKFQYIGIVWLPVTWLALVVELSSYRFSKRIFA
                     VSSIPPVIFLIFIFLVPQPNAFWGIPTLEDTSLFPMVNFHYGPLYYFGFLPFSYLMAL
                     ASIVVLWRNYGTDHVHYVRQKLLLTFVLLLPGLTNLLYNFNLSPVRGLNLSTATLTVS
                     GVLFYIILYRNSLLDLLPVTREQIVENLDEAVLIFNPQKRLIDFNHSAAKLFNLGRNS
                     IGTGVEEIGVKELTHRLENLQDAESKTLALMQGLVYDLSLNFVRDPSSEFCQAIIVTL
                     RDITQEQLMNQRLQILAERDSLTGLYNRHTFFFRSKELIKTNPRVRIAAIMIDLDNFK
                     AINDTYGHAYGDLILQTMGEALLALTSPTIVAGRLGGDEFVVMTTEQDLSDLASRLSK
                     KVYDRLNVVVKEGLNPTLSVGVSSLNLASCTVEDPLEYLLNQADLAMYQAKRGGKDKI
                     VFTGDFIPG"
     gene            22441..24747
                     /gene="xdhA"
                     /locus_tag="PLF67_01995"
     CDS             22441..24747
                     /gene="xdhA"
                     /locus_tag="PLF67_01995"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HOE88817.1"
                     /translation="MKIIGSSPNRVDAFDKVTGKAKYAPDLLPPHALEAKVLHSTIAN
                     GWVREFDLSEAWKVEGVVDIVTCFDVPDIQYPTAGHPWSNVPAKQDICDRKLLNTRVR
                     QYADDIAAVIAETELAAIEAVRKIKVVYEELPVILDVKESLENTEFPLHEERPDNIVN
                     KTSYEIGDFEEAIKEKGLTRISGTYETPVVSHCHIEPANSFAYEEAGKIVVVSATQIP
                     HLVRRMVGQALGVDWGKVRVIKPYIGGGFGNRQDALTEPLNAYLTTRVGGRPVRLAYS
                     REETFECTRTRHAMTFDLTTYVRKDGSFAARDLTVYSNQGGYASHGHALAANAANAFR
                     MMYPVGAIRGKAITVYTNMPTAGAMRAYGIPQIAFALESHIDDIGKATGLDPLLLRTK
                     NMMPLGYVDGPTTITCHSTGLAECIAKGEEHLDYSKKRLAYANQDGPIRRGVGMAIFC
                     YKTGVYPISLETSAVRMILNQDGSMQVQVGATEIGQGADTMWVMMTAETVGVTLDKVY
                     LVSKQDTDVTPYDTGAYGSRQTYVSGMAVKKTAESMRRKILEYAALMTNKDPMDLDIV
                     ANKIVRKNTDIVLTTVKDVAMESCYSLTNSNHIAVEETHHCSDNTYAFGVCFTEIEVD
                     IPLCQIKVLDIINVHDSGRIINQTTARGQVHGGMSMGLGYAMYEYHKFDPKTGRMLNG
                     NLLDYKLMTPLDTPTLHAAFVETDDPTGPYGNKSLGEPPTIPVAPAIRNAVLNATGVS
                     INANPLHPERLFDSFVEAGLIKQRSEHV"
     gene            24740..25627
                     /gene="xdhB"
                     /locus_tag="PLF67_02000"
     CDS             24740..25627
                     /gene="xdhB"
                     /locus_tag="PLF67_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HOE88818.1"
                     /translation="MYNFKTLYEATSVAEALKLKADHPEALFLAGGSDILIKIREGRL
                     DGVDLINIYMLDELRGICLEEDGSILIRPLTSFTDVAMHPVIKEHLPVLGEAVNTIGG
                     PQVRNIGTIGGNICNGVPSADSAITLKAYDATLEIVSTEGTRILPYADFNLGFGKVDL
                     GPTELLAGIRIPKASWENTFGHYIKYSMRKAMDIATLGCSVNVKLSKDKQAIDRLRIA
                     FGVAAPVPVRSPKAEELAKGKNITQALIDQIGEAVLGDVNPRSSWRAGREFRLQLIEE
                     LAKRATIEAINKAGGTINV"
     gene            25620..26108
                     /locus_tag="PLF67_02005"
     CDS             25620..26108
                     /locus_tag="PLF67_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOE88819.1"
                     /translation="MYNRSVTCTVNGKKRTFMIDIRSSLSDMLRGEAGLDSIKHGCDV
                     GECGACTVLIDGKPFNSCIYLAIWAEGKTILTLEGLSTTKGAISDIQQAFIDEGAVQC
                     GICTPGFIMASIPIIESDVPATRDEIKRQIAGNLCRCTGYEHIVDAIEKTQKKRIAER
                     TR"
     gene            26142..>26457
                     /locus_tag="PLF67_02010"
     CDS             26142..>26457
                     /locus_tag="PLF67_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88820.1"
                     /translation="MNILSNIPASVQPYVPALGVLFLATIVLLVTRAILKHRIKVLNA
                     REPKPRYLTVLELVHKILLPVLFLVTLSFALTLTQFSTTVRKIINISFLMIGTYIAIR
                     AIN"
     assembly_gap    26458..26557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26558..27064
                     /locus_tag="PLF67_02015"
     CDS             <26558..27064
                     /locus_tag="PLF67_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE88821.1"
                     /translation="DLFSYFVILFDHPFGIGDYIVFDDKSGTVEKIGVKSTQIRVKNG
                     EILVVANKDLTASRIHNYKRMEKRLLVATHNFPFDTPYEKLEKIPSLIAQVVSSLTTF
                     EGITLDRSHFKTIGKATYEFESAFYVPSPGYIDLLNVQQEMNYKLVKIFEEEGIRFAI
                     PTYKLQED"
     gene            27206..30319
                     /locus_tag="PLF67_02020"
     CDS             27206..30319
                     /locus_tag="PLF67_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE88822.1"
                     /translation="MSSLFLSHAIWQHPQVTSLNRLPMTSLPLFFPTLEEALSDALGG
                     PEKRDLSDHPYHKSLKGTWDFILYDNPLSVDERVLDEKNSLSWKPVIVPGSWSIQGFD
                     KPHYTNVIMPFEDDPPHPPQVNPTGVYRTAFTVDNHWKNRRTVLEVGSAESYVELYLN
                     GAFVGLSKDTRLEACFDLTDYLKEGSNTLVLIVVRYSDSSYIEDQDQWWFGGIHRDVT
                     LTSLPEKTLSDIKVTPTLNEALTEGIIEIRTPGNQVIEALDVRVYTDAGKLIEQEILN
                     AEEGVIVGSLKVASPKLWSSETPNLYYLGISSLNEHLVIPIGFRTIELHRGNLLINKK
                     RVLIKGVNRHEHDERTAKTISVESMVHDIKLMKQHNFNAVRTAHYPNDPRWYELCNRY
                     GLYIIDEANIESHSNYDALCREESYAAAFLDRVQRMVRRDFHHPCIIGWSLGNEAGYG
                     ENHDAAAAWIRRYDPLRVIHYEGAVRPEWGQGAHTLESLKRGSFATDLVCPMYPPIAL
                     IEEWDRTTDADRDPRPLVMSEYSHAMGNSNGSLSDYWDLIRSSRSIQGGFIWDWVDQG
                     ILVDDEGKPVGFRNVEEHVPKRWRYGGDFGDQPTNYDFCLNGIVFPDRSLKPVMSECL
                     KVHQPIHVRSSNPSSGYFTIISEYDFITTKDIALTCSIYNESSSEEYTFGVELPILQP
                     GGQAQIHIRELQESRVRKMMGSSTTFIRFVCTLKEPAPWAPKDHIVALEQFVLSTISK
                     SLHIHAPAYPIKQTDDSLFVETILYKASISSEGFLSALHFEDRPPLLTSPLRPSLYRV
                     PTQNDGLKTYTHLEGIKEFSFYHEKKAMKPWLEHSLNDLVLILEELKIRHNQVKTIHQ
                     LKTHTGLDIGSVRQTFTFTLEEVFLHAIFDLSDAFAEYPKVGYRCSLTKQEEPVSWFG
                     RGPEENYPDRKTGSLIASYDKTVEELFVPYIVPQDCGVRTETTRLVLEGAKIEGILPF
                     SFSIHPYSPEELWEKRHADDLVVDEVDHLSLDAAVRGVGTATCGPDTLDRYRIPGGIY
                     QMRLRLS"
     gene            complement(30339..32342)
                     /locus_tag="PLF67_02025"
     CDS             complement(30339..32342)
                     /locus_tag="PLF67_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88823.1"
                     /translation="MQNVTLFSEDFSSFDLGGFPYDPEHSAMGEYHYYPAQGYKGFWY
                     DPIVNWNYTGPSWIVTNPRLDGVNVMELSRIQEPKERDVLPTLTAGDRDWKDYQLKVA
                     LRPLSRELFSGVLVRYQTSLRHYGVFFTDDGIEVHKVDLTERTVLAKASAPWLTDEFQ
                     DLIVSVHGNTITVTLNGEWLLEVFDDWYDRGAIALSGCMPTQYAHVEVMTSEKEALRL
                     EEERIEKRRKLAQKRANQPKLAVAKKIDLQDFGSGRQIRFGHLTGTKEYFFVMAQSQR
                     RVYKDRYPMISCLTAVSLESGNVLWQLGKPRTDDGVIKLTTDLPFQVYDIDGDGIDEV
                     IASWDFTLYILDGRDGSVKRRIPTPYNVEPVEELDGIEFGHHAFDRLNVDAIRIVNVT
                     GKVRPSDILIKDRYARLWVFDDELNLIWKFHHNNTGHFPYARDFNNDGKDEIFSCYNM
                     IDSEGKLMWKLPIDSDHTDEIIVGPIDPDQEDLIAIVSGWEGFILLKPDGTILKRTIN
                     GHGQRISVGNYIPENRGLEICTTTYWGAQGILYMHTCKGEEIWSKEMRSNGALITPVN
                     WDGSGRDLLLIHSGVEHGGLMDGDGDIVVTFPDDGHPELCCEVLNILGDSRDEIIVWD
                     QKELWVYTQDREQPEREYSYHPVKYSTYNASNYRGEYSFPRWE"
     gene            complement(32362..33654)
                     /locus_tag="PLF67_02030"
     CDS             complement(32362..33654)
                     /locus_tag="PLF67_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE88824.1"
                     /translation="MSVKKRYVQVGTGGRARMFYEAIATVYRQECELVAFCDMSQVRM
                     DFSNRILTDECDHPAVKTYKHTEFDRMIDEMKPDVVIVTTVDRTHDYYIIRAMEKGCD
                     VLTEKPITTDEVKAQAILDAQKRTGRSIRVTFNYRYAPHHSKIRELIRDGAVGEVYSI
                     HFEWLLNTEHGADYYRRWHRNKVNSGGLLVHKSTHHFDLVNFWMDTQPKKVFAMGALN
                     FYGQAAAVKRGVTNFSYRYHNSPYAKDDPFAIDLESSPVLKGLYLDAEEESGYIRDRN
                     VFSDEISIEDTMGVLVKYRNGAILTYSLNSYMPWEGYNVMINGSKGRIEYSAMEKPYI
                     NAGGKKSDEGAMVYHHIKVLPLFEAAYDVEVEVREGGHGGGDPAMLDDIFLENPPHDP
                     LKRSADHTAGLRSILTGIAANKSIASGLAIDVDSLLVW"
     gene            complement(33743..34579)
                     /locus_tag="PLF67_02035"
     CDS             complement(33743..34579)
                     /locus_tag="PLF67_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE88825.1"
                     /translation="MSVRITSSGRFYYKEGQGMVEHHHPDDYQIQLVYSGTARNYFNS
                     EAMDLETGDIVFCKKGCYHAFHATSKDGVKMLEVKFTTESRKPKDVLEGINTRFRDRE
                     NQLYLLLSRIVLEGQRKMVHYKQMSSVLLMETLLTMNRICLEQVLPVYDSNPIHQLRS
                     GSLTTKNDVLDAVDAYINNHLGDSFTIGEMADECGYNQDYLYRVIKKQTGLSAIKYIN
                     LMKFERSLSLIQDTDLTLSEIGWKLGFENLQYFSRFFKRHGGVSPSEYIRKVRHTTRT
                     DY"
     gene            34773..36251
                     /locus_tag="PLF67_02040"
     CDS             34773..36251
                     /locus_tag="PLF67_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE88826.1"
                     /translation="MKKTKRLLVTLSILLIALVALAAAGQKDSKSSSIPTITLATADN
                     TYGLSTDPELQGAITALLESKTGTKINPIIPPLASYTDKLATLINSGDIPDLFVIAQA
                     MSKVPTMVARGQLLELTDYIKKSPALSALDPNLFKDLQIDGKIYFVPYNYPKSKALFL
                     RKDLMEQYGVNLSSTPTTEEFRREMAKFVGTGVVPFCFPKWVDNFQYFYNSFGAWGGV
                     YLKDGKYIDGFQTPEMEAALSYLRQLYVDGILNQEFLTTENSHMREKTYTAQAAASID
                     YVTNYVNYVQNTTAANKPTEMHLIYKVIGPNGDGGSLNEATQTALLVSARTPYPDAAV
                     KVLETIVTDPEVYPAFFGIGLEGKHYTLNADREIVPTTQAANSGYKYTLNYLSDSFID
                     IDINNLPFALTKNLQQALPAQIGHIKTMAGHLGPNHAADVPVGISDAYDRVVPSIKST
                     RESIAAKIIVGSVTLQQGMAEYENFWKSINGPKILEELNASL"
     gene            36329..38425
                     /locus_tag="PLF67_02045"
     CDS             36329..38425
                     /locus_tag="PLF67_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOE88827.1"
                     /translation="MTPQQRAEHLVENMSIEELLSQLRYNAPSIPRLGIPEYNWWNEG
                     LHGAARSGTATVFPQAIGLAALFDPVFLYEIASIISTEQRVKYNLYTKEDDRGIYKGL
                     TVWSPNINIFRDPRWGRGQETYGEDPYLTSILAVAFIKGLQGEGPHLKTASCVKHFAA
                     HSGPEPLRHHFNAIVSDKDLEETYLPAFEAAVIEGRVNAVMGAYSAVNGEACCASPFL
                     IKETLRGKWGFTGMFISDCWAIRDFHQGHHVTTSVEESAALALNAGCDLACGNEYEAL
                     TTAFEKGLVTVGTIKQAATRVMTTRYALGMPGDSTEYDHLGLEHLDSDNHARVALEAS
                     RRCLVLLKNDAFLPLARNKFKTIAVIGPNADNRTALWGNYHGTSSRYTTILEGLRSYT
                     KGFATIRYSEGSGLTKRNVERLAQADDRVSEAVFMAKNSDLVLLCLGLDERVEGEMRD
                     DGSGGWAGDKSDLRLPEAQRILLNAVAQVGKPTIVILLSGGAIDPEIEAYPNIRALIQ
                     GWYPGQQGGQALAELLFGEYSPSGKLPVTFYKASAQLPDFEDYSMARRTYRYCNHDDV
                     LYPFGFGLSYGSFSYSLLTVQEGENDSVIVKGLVTNTSAIPARTIAQLYLRVSSSDAP
                     PHPVLVGVNSIHLLPHEEHVILFTVDKKHFTMVDEHGTRYPVYGRYTLFFGSSQPDKK
                     SKELGAEPYDRLDRIR"
     gene            38453..39358
                     /locus_tag="PLF67_02050"
     CDS             38453..39358
                     /locus_tag="PLF67_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE88828.1"
                     /translation="MRRKKTTFLTNLRNQKYLLLMLMPGVIWYLLFKYLPLLGLSLGF
                     TDYGFRAKVSFVGLDNFKRLLTSTIFWNAFRNTLIISLANIIFYFPTPLIVALLINEL
                     KSVKTKRFIQFLIYIPYFFSWVVVGSIFVNLLSPSTGLVNSVITKFGGKPIYFMASVK
                     HFRPVLITSYIWRQMGYGAVIYVASLTTVQPELYEAATIDGAGHWGRLWHVTLPAIRS
                     TIVTMLLLNLSHVLLIFEQVLVMYNAAVYGVADVLQTYVFREGVLSGDLGYSIAVGMF
                     TSVISLTLVLSTNKFSSRFLEEPIL"
     gene            39368..40234
                     /locus_tag="PLF67_02055"
     CDS             39368..40234
                     /locus_tag="PLF67_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE88829.1"
                     /translation="MIKSNTIRESTSRKVFRAFNMIFLTFVCLIFIIPIWNVLITSVA
                     KDIDVMGSDYLLVPRSFTLQNYWRVLNSGYMGAFKNSLKVAVLGTIFSMLITVPMGFA
                     LSQKHLLGRSIIMKAIVFTMVFDVGIMPFYIVVRSLGLINKTAAIIIPVGLSTFNLII
                     LKNYMGSIPVSLLESAKLDGCNDATILLKIVLPLSVSILAAVILFYFVSYWNRYFEVI
                     MFINDSRKYTLQVVLRSLMFESDDSLGGGQYVYNNLKMAVMVLGMLPVLIIYPFVQKH
                     FVSGIMLGGVKG"
     gene            complement(40275..40622)
                     /locus_tag="PLF67_02060"
     CDS             complement(40275..40622)
                     /locus_tag="PLF67_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE88830.1"
                     /translation="MKYENFVLNSERKANAVGKGMTRKILAFSKGTMLCELHFEKGAV
                     GSLHSHPHEQISYVVSGAFEFDINGVKKIVRAGDSLYKEPNVVHGAVCLEEGILLDFF
                     SPMREDFLPELED"
     gene            complement(40647..43013)
                     /locus_tag="PLF67_02065"
     CDS             complement(40647..43013)
                     /locus_tag="PLF67_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88831.1"
                     /translation="MRFISHYLTSPLVEKAYIPPVRTHASLMRYEKHLRSLIAHKDLA
                     YPDEEQKWSYYYSHGNWSVDFSTFYSTLTYVSFDAKTYLQPPKRGSYSLTLWTYAAVT
                     VWCNGRKVCSIEEPVYKPIMKRAFTVELGEGRNELYIRLQTLGVRDTRMLFGIEIPAE
                     LSTVLTGEYDEHLAAASFLDNVRLEGETLFFPEDRGLIVLAHDEASPDFARVPTRYRR
                     TTVSTDRPLKVAGPEAAFFLRVPVGGGELSRRFEIIERQKPEYTNVHDRKENYRRMLR
                     VIADADGLSRGDKFGFYIQNILARKALGIEHPRDREYFFTTLQQIEDRYDCSDFLVSG
                     VIRYMKSYPIDDELKARIDEVLINYRYWMSMDGSDGMCFWSENHSILFYSSALLAGEM
                     YPTTVFPTARMTGSELADFGRVLTNEWFDDLEELGFEEFLSTVYMNVTFAALLNIYDY
                     AEESISKRAQGVIDRLLHDLSLHTFDGSIIAPMGRVYRSVITPSKQGAQALMNLVNPK
                     VPTTFGEGWLSYYATSSYPIPNGLVDVMQKPAEVKYSSGNALIMLKKERDYCLTSVQS
                     PRTDGVERWKNITLGDTDYEGKPIHLYTKSFNERFHGTTFFEPGVYGYQQHMWSAALS
                     NETIVFVNHPGGTHDSSSMRPGYWFGNGILPALRQEGAMLATIHRISDAHPIRFTHVF
                     FPKAKFDSVEREGRWVFARKGQGLLALWCSRELVAHNDELADCELRAYSSDHAYVCVV
                     GSIKEEGSFEAFQNRMKALEPHYAQSTLTLEGKPFLYYEHADDETQFV"
     gene            complement(43017..43883)
                     /locus_tag="PLF67_02070"
     CDS             complement(43017..43883)
                     /locus_tag="PLF67_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE88832.1"
                     /translation="MSSANYIRSNKQKAVIWALTIFIGFCALLLLYPVIYISLGSFKE
                     NKELLLGGANLLPKRFVLENYTQAWQNANFSVYTKNSFFLGIGTMILTVIISSMSGYV
                     FSRKDFKYKELLYSTFVAFMFVNVGSVTLRPLFELAVKLKMNTSLVSVVLILVGTGQA
                     TFIFLNRGFVNTIPKELDEAAKIDGCTFFQTFYRILFPLLKPITATIALLSFRGGWNE
                     YILPLVFTMSNDSMRPLTVGVNMLRNVGDGAAAWNIVFAGATMAILPMIVIYLIFSRH
                     FMQGMTAGAVKG"
     gene            complement(43894..44742)
                     /locus_tag="PLF67_02075"
     CDS             complement(43894..44742)
                     /locus_tag="PLF67_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE88833.1"
                     /translation="MLSPAVLLLVITSVYPFFWLFRYVLYDYNGFVAYFTGTYNIKRA
                     FTDAVFWKSVLHTFEYAIGKLILIIPISLGLAVFISRNLPGSNTFRIIFFLPTVISAS
                     VYSLIFTFIFAVFRGPLNTVLEAMNLITRPIDWLGDPKWVMKSIIVVAVWGGLGNYMI
                     YFISGLSSISEEIYESCMIDGANGTQTFFKITLPMLSPILKVILLLAITTAFKDYQSI
                     LVLTNGGPDGRSHVMFSYIYNLIFAAFSSPQIGYATVLSVLAALIIGVITAGYLYLAR
                     KLDDVF"
     gene            complement(44875..46248)
                     /locus_tag="PLF67_02080"
     CDS             complement(44875..46248)
                     /locus_tag="PLF67_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE88834.1"
                     /translation="MKKVLSILLIFLFATSMMFAGGAGESQAPKGKTVVEIWSNDRHD
                     LEYLLAKVSEFNAKSDTIEIKHTTVVENYPNMLVMGFTSGNAPDLFYMNARGTGFDLK
                     TFVDANMLTPYPDSLLDDPEFAKVTDARKNIVEGVNAINGVPYAIFNVRRSGTRMIYN
                     VDLFKKAGITKFPTTLAGLVEAADKVTKVGNGQFYGIATCASAQFERWLEGIINKSGV
                     YHYDFKKGVFNFDGYREGILLAQKFFSNGSMFPGSNTQGVDAMRAQFANGTFGIWGNA
                     SQEAGVFTDQFPIEDFEWAVAELPSLDGTVKGTVDSRPQKGLFMFSSSKNKDAAWEVV
                     KYLNSEEVVKGYIEKGYALPLSTYMQKVVDMSGAGRLGDFAPVPYEGLYPSVPAVTVA
                     GDNYAVSIWNAIVNGSDVDRLIADLNKRYNEALERDLKIGKVKRIVIPNFDPLNPNKE
                     KVQFLDK"
     gene            46385..47893
                     /locus_tag="PLF67_02085"
     CDS             46385..47893
                     /locus_tag="PLF67_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE88835.1"
                     /translation="MDSELRILVLDDEPPIRKGICTLITHKLPSVHIVGQGSNGLEGL
                     ELYRSTQPNLIITDIVMPEMTGLEFLREVRKADLNTPCIVISGYDEFSYAREAIRLGA
                     TDYLLKPISSTELIRAIIKATDSAHEAKHTDYEELRSSASTLFLHRLLNGELVEIEKV
                     LQACKTLEISLQEGAVTVITLASPNAEEITRALQWAQTRAVTVTQRGPHGVLFIQGSA
                     TRADHLAADILSLNLPALRVGIGQPVTTLSSVHISFESALQALTYQAYYPHERLFTQE
                     MIDRSTPYLDSTDIDTLRLKEILLLGDEDELVTWFNTFFSSLFYIKTPPLSYLKGMCI
                     YLLTTVHKHLGEDLGITKSYFDRVAPFNASTVVSIDDLYNEVLNHLVVLNNKVIVQAI
                     ADSDIVIHQAKQYVENHLTEIISSTEIAEKLKMSPTYFSTYFKTKTGQTFRSYVNSRR
                     NEYAKSLLANITMSIEEISEQLGYTDYRSFHRIFKRMNSITPSEYRKRITKR"
     gene            47897..49645
                     /locus_tag="PLF67_02090"
     CDS             47897..49645
                     /locus_tag="PLF67_02090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOE88836.1"
                     /translation="MKKIRYWYSSLPLSSKITLLMALLITLLAGSFSLFSISFFNQAY
                     SHETEQIARQRLELFAENLDRDLLAINNQIIETSTMPLFVRTIQRALEVPSKDLETLV
                     NFQQFFKRIADSSPLIHSVHVITKDDILYSPYEQTSRTLGNDLSYPSLSHLQKVTLLP
                     GGQNPFAPSRKSVPIVVALNLLGTSSYLELTSNLAADVFLIILLDEQALLEKINSPGS
                     AFFSYTNTLLFQGVPLLPEAPESDSAMIRFATPTQINGLELMMSIAKRSYKPLVAFMI
                     IITIITALLVTGGGSVLISRISRHITSPFSAMTKMVKEMETGSYEFDIKPRYADETGT
                     LIEGMNSMYHTIIQQMRQIKEEEQQKYRYLSQMLTEQINPHFIYNTLEIINMEVINGR
                     QENASDMIATFAAFLRHTLNQGEDTTTLAQELEHCRKYLSIMNTRLGLQILIFDDIAP
                     HLELFEIPKSILLPLVENAIRHGFETSSMQAQTVEHPSITITAKQKEDRYSIGVIDNG
                     TKGIDIEQAYKALNEKSAQSGHIGLNNVLNRLKLYFDDVEAHVSSIPGYRNAITFTFS
                     IDPDRGAPSGIAALAE"
     gene            complement(49592..51166)
                     /locus_tag="PLF67_02095"
     CDS             complement(49592..51166)
                     /locus_tag="PLF67_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88837.1"
                     /translation="MKTIQQVFAASVSAAQKQWLEEHRMYLGDLHNHCAISYGHGSLS
                     KAIAFAQSQLDFFTVTGHFAWPDMEDGSQPIPQRVVDYHKEGFAKLRGVWEEYLEAMR
                     LSASGGIVPFVSYEFHSFKLGDYTIIRKDLYEGFPPDPEGIDTRLDEYLATNDPRKSG
                     IICMPHHIGYKKGYRGINWDRFNETASPIVEIMSMHGASESRYATPAYLHTMGPVCGE
                     NTMQGGLARGFRFGVVGSTDHHNASPGAYGSGRAGVWAKEKSQDGIWDGLLAKRTIAL
                     TGDPIRIASFLEDRPIGSVVKATGKPMRFDSYILAPSALAYAELLVDNLVVKTFRPNP
                     ARSSSKRFNLSLGWGQRDVSAHWKVTLRAEGAAIVDPVPRFKGEYVVDPLSVPTDEAL
                     AVPSLRFDGVSISMSCNTSGNISATTDNTQGFGFGLETDGPYSIILHVEAEVAGETFM
                     HDLLLQSDELQHGPSVLYTHGFVSPALRLSEEIDLSECMLEIHESLDLDSGSSLYVRS
                     FSQSGDTAWSTPIWID"
BASE COUNT        12140 a        14185 c        12822 g        12085 t
ORIGIN      
        1 ggatgatcaa catcgcgtat agttgccagt atctcaagaa atatgctttt gctttttcct
       61 gtttcttcat cacacgtccc ccctgaaact tacagattcg agttatacag ctcgaggaac
      121 cgatcgattc cgagattctt catctcctgg acataggcgt tccaatcgcg atcgatcgac
      181 ttcgccccgg tgaggaatgc ggagttccag cgttcccaag cgtcgtggag cggagtctgc
      241 agcgatccgg cttcctctgc ctgtttgtca tcaaacttcg gagtcggcgg gatgtagcga
      301 atcacatcac ccatcgcttc aacttcagcg ttgattcggg cgtagttatc gtcgtacttc
      361 gtcatctcgc gggcgttcac ccagaccatc tgcgttacat cgcttccggt tccatgcttg
      421 acctgcatgc tcttgtagat gccttccttg gcgttcacga actcgtcggc gaacacgatc
      481 ttgccattct ggaccgtgta ggtgacgcct tcgacaccga gacaccagag cttggcgttc
      541 tcttcggaga agaacatctc atccaccgct cggacgagct gctcgaaatc ggcacgacgg
      601 ctggttttga tcgggaacat gacaccgcca ccggttcggc tcttctcctg gtggtgagct
      661 ccggccggac cggcaagagc cggatacatc tgcagcttga agcccggaat ggtgctgttc
      721 gcttcgactc cgccgatctg gtcgtagtac gcataggaag ccatcgcctt gccggttgca
      781 agcttctggg tccagcggtc gccgtcgatc ggctcggcca tctcgggatc cagcagacct
      841 tcggcataga gcttgcggaa gaacgtgatg taactcttgt actcttcgct gatcgctccg
      901 gcaaagtact gcttcttgtc gtagtcccag ctcagggtgt tcgtgccgct cgcgccgttc
      961 ttaccgaggg agactcccca tgagggcatc gtcatgcgat agagaacccg cgggcctgcc
     1021 agaatcgtga gcggatagga gtcgggagca tggcgcttga actctttcag cacgcgatac
     1081 agatcgtcaa acgtcttggg tacgggaaga ttgtactttt tcagcagatc ttcgcggaga
     1141 atcaatccac cgtcgtagaa cggtacatca aagagagccg gcatgtaata ccgtttgccg
     1201 tctttgagtt tcaacagatc gacatcgttc tccagaccga actccttgac tcgagcgttg
     1261 aagtgagggg tccactccgg ataatcgctg atcggcacga tcgctccgtt gagcgcaaga
     1321 gccgcatttt cacccttcgt tgactgatac aagatcacat ccggagcgtt ggttccggtg
     1381 ttcaacgcaa gggagacttt cgttttgtaa tccgcgatcg ggatgatctc ccaattcacc
     1441 ttcactcctg tccgcttctg aatctcggtg accgtgagcc acgtatcctt gaacggaagc
     1501 gttgcattat cggagtagaa tacggaaaac tccaagggtt taaccacctg ttccttcgcc
     1561 ccttgagcgc ttacgccaag aaggacacat gtgaccaaca cgagtgtgat acctaggact
     1621 tttttcatag ataccctcct ttaagttata tgtgaaaaac ctcaaccggt ttctcattca
     1681 ctgctatggg atgcatttca tccccctatt catcatcaaa aaccaatccg cttccgaggt
     1741 aatcccgata gatgcccgga gtcgaacctt caataatcgt cgctcccacc gttttctcat
     1801 agtactcggc cggacctacc ccgccgatga tcgcatacgc atacccttcc gccttgaggg
     1861 cctccagact cgcgatcagg agcgcttttc caatcccctt tccccgcatc gatttcagca
     1921 catgcgtcgg accgaagaag ttcttcgccg tcgcgttgta acaggcgaac cccagaatct
     1981 cccgcttcct cgttgcaaga aagagagaaa taggctgatt tgcaaagcac acgtccgctt
     2041 cgcttcttgc ccctgccgtg gagtgttcac ccacccagtt caatacccgc tccttgtcgg
     2101 gagcaagggc tcgatggatt cgaatcttct ccttcgcaag atccttgagc agctcgcttg
     2161 agccgggaag ctcatagagt ttgaccagca tgtcaggcat cggttcttcc tcccaaatga
     2221 accggcagga gaccggtcgc ttgaatttca cctttcaaaa cccttgcgat cgcttcaaag
     2281 gtcatcggag tatacgacca tgcggcgtag gaacgaatcg aaggattcaa ggaggaaagg
     2341 tcgtagggat tccgcaaggc gaatacgcac acctgagtta cctcccttaa agcatttgcc
     2401 aaggtcagct gtccgcgatg gagatggccg ttatacgttc caagcaccac actctgatag
     2461 ttctttgcct gcttaacgac ctcgccgatt tcatcttcgg tcggattgtc cccgacaaga
     2521 aggtggtcgc cacccatcag atccgcaaga gcggccccga agtgtcgctg ttttccgtca
     2581 tcggagacgt tcgtcgcccg gaacggacga ggtccgatga acagggggtt cgctcccagc
     2641 gtgaagggag catcgttcac caacgtgatc gactgttcgt gaagctcccg attcaggcgc
     2701 gtatgctcct tcgtaccgat gatcgaagag ggcttgaccg gaacctccgc caatctcttt
     2761 ttttgcctga ggatcttttg agtactcttc ttgagctcca tgaactcggt cataccaaag
     2821 gttccatcct ggatcgcctt ctccaacagt tcgaccactt cgacacccag attgacatgg
     2881 tggctgatgc agacgatatc caccccggct cgaagggcca tcaacgctcc gtcgaccgtc
     2941 ccccagttgt cggcgatcgc tttcatctcc atgcagtcgc tgaacaccaa gccttcaaaa
     3001 cccatctcct ttttcagaag atcggtgagg atcgttcggc tcatcgtcgc cggaagattc
     3061 cgatcctcaa gctccggaaa gaggatgtgg ctcgtcatga ttccctcaat cttattgttt
     3121 atcgccgctt tgaacggctg gagatgagcg gaaatgtctc ccgtgacggt cggtaggctg
     3181 aggtggctgt cgatatgggt atcaccgtgt ccggggaagt gtttcccgca cgccatcacg
     3241 cccccttcct gaagtccccg agccatcgca acgccgaatt cgctcaccac tgaagggtca
     3301 tccgagaagc tgcgcacgcc gatcaccgga ttgtccgggt tggagttcac atccaaggtg
     3361 ggagcaaaat cgacgtttac cccgcatgag cggagctcgg ttgcggtgag gaatccggca
     3421 tcataggcat tcctgctctt gcccgtcgct gaaagagcca tcgccccggg gatgttggta
     3481 aaatccgaag agagacgggt aaccatgccc ccctcctggt ccacgcagat cagcgcatcg
     3541 gtatggcatt ccgagcgaac cagcttttga atatcggccg tcagcttcat cagctgttcc
     3601 ttgctttcga tgtttcgagc gaagaggatg acattggcga tcttgtgatg tgaaaccgcc
     3661 ctgcggaact cttcgcttat ctcggttccg gcaaacccgc atacgagatg ttgtccgatc
     3721 atcagtcgca gttcttctct agtcatatcg gtaatacctc tttgtcgctt cggcgaaatc
     3781 cgcgctctcg gccttcatcc gctcgaccac cttctccgga tcctcaagct cggcggaatc
     3841 gaagcccgcc aacgccctga aatgcttggt gataacaacc tgtttcggat acatccgaac
     3901 gatcagcgaa agaattcgca ccgccgtttc gacgggcata aaggtgcgga catccgtgat
     3961 atgaccgtac aggccatgga caagatcgcc gccatggggg gtgtaggaga tcggggtgaa
     4021 cacgaccccg tccaagtcca gtcgcctcac ctcgtccgcc aatcgattcg aatcgatgaa
     4081 cggcgcaccg agcagctcaa acggggcgct ggtgccccgc ccctcatgca gattggttgc
     4141 ttccaacagg cacatcccgg gatagaagaa gagaccctcc tgatgggcaa tcgccgggct
     4201 ggtcatgacc cacggaacga tcttgttcca ccagaggtca tcccgccacc agggaaacag
     4261 gtcgatcgtc gtaaggtcgc accccagctc atattcttca ttcatccaat aggccagctc
     4321 cccgatcgta agcccgtacc ggatcggcag ccccctcggc ccgacgaagc tttcatcctc
     4381 agggcgaagc acgacccctt cgacgacccg tccccccaac ggattggggc gatcgatcac
     4441 ggtgacgggg attcccgtct ccatgatctg tcgaagggtg gagatgtagg tgtagaaccg
     4501 gaccccgaca tcctggatgt cgaagatcag atgatcgaga tcgtcaaagg tcgcccgttg
     4561 agccttctct cctcgataga ggcttcgaac cggaagaccg gtaagcggat cggtcgtgtc
     4621 cgcaaccccc tcgccccgat ccacatatcc gccgaggccg tgttcggggg cgtacaggca
     4681 agaaagcccg atcagttcat ggagatggag ccggctgctc ttccaatcgg gggtgaagct
     4741 gctttgattc gttaccagag cgacccgctt gccgcgaagc ggggagagaa cggatttcat
     4801 catgcccttc cctccttgca ataccgatga tcctcttcca tactcatggt atggaaggaa
     4861 tctatctggg tggagacggc ggcggctcga cctgtcgaat cggagcgtgg gaccggcatg
     4921 gcacgcttat gacccatgcg acgggctctt cgagcaatgc gtatgtcgtc ggctttgacg
     4981 aagcggtccg gaccgtccgc ttcctgatcg aagatatccg atcccgcctc gaaggtcggc
     5041 cgattctgag cttctgtttc ggcaactccg gtcttgcccg ggcggaagag cggactcgct
     5101 gggaagcggc cctcaaggag atcctcggaa acgaggttcc ccttctgctc gtgacggaca
     5161 tccacctgct gctcgcgatg aacgatcctc ggcggcttgg cctgtgcctg gtcagcggca
     5221 ccggctcggt agccctcgga cgaaacgaac atggatcgga agtccgcagc ggcggcatgg
     5281 ggtcggcgct tggcgacgaa ggttcgggct ggtggatcgc ccgcgaggcg atcggtcgga
     5341 cccttcgttc cgtcgaaaag cgggatctgc ccacccggat gggaaaagag ctgctcgctt
     5401 tcttcaaact cccggattac agccaagcga tctccttttt caattcctcg gagcggacaa
     5461 aaagtgaaat cgcccgcttc accctgattg tagcccaagc tgccgaacag ggagatctcc
     5521 ttgctctcag catcattgaa gaagccgccc gtgaactcgg gcttctggtt tcgtcggttc
     5581 aaaagcgcct tgaggaaccg tatcccaaac ggctgatcat ctccggcgga acgatcgtgc
     5641 acaatccctt gctccatgaa ttgcttctca agcatctgga tgaacgcctt gaaatccacc
     5701 ttgctgtgga agggggactt gaaggggctc ggatcttggc acaacaactc attacccacg
     5761 gctctgcttc ctatgagtga ttgccatcgt ttcttcaatc gcttgaattg aagcatcgag
     5821 gttctgcgat accaacaggg aataaaggat atcgatgatc gccagctggt tgattcgact
     5881 cgtacccgcc ccctggcggt aaatccgctc ggtcgccggg acgcgaagtt tgtaatcggc
     5941 aaggttttga atcgtgttgg gattctccat cgtcagggcg atgatcgggc agcctcgctt
     6001 gtttgcctgg ttggccacct ccaccatcac ctcggtttcg ccggaatagg agatgatgaa
     6061 ggcaagatcg tttgcggtca acgtcgaagc gacggtgagc tgaaggtcga cctcggcgga
     6121 ataaaacacc cgcattccca accgagtgag tttttgcatc agatcgagag cgaccacccc
     6181 cgacgccccc accccgaaga ggatgatcga ttgagccgct cggagagctt cgatgcatgc
     6241 caagaggacc tcttcggaaa cgagagcttc aagagagccg agggctcgtt gggtctgagc
     6301 gataaccgtc cgggttaccg caagcggaga tgtctccgat tccagatcga ggtttggcgt
     6361 gtacagttcg tcaaacgtgt caaacacgtc cagagcaagg cgaatcttga agttgttgaa
     6421 gctgtcaaaa ccgatatgcc ggcaaaaccg ggtgatcgcc gcctggcttg ttccgctttg
     6481 cttggcaagc tcccggaccg tgaagtggag ggcttttttg ggattggcga taacataatc
     6541 cgcgactttt cgttccgaat cgctgagatt cgcatatttg ccgttaatct ccctcagagc
     6601 tccggcgatt tcgtactcaa ggtgctctgc gttcattcct ggcccttctc ctctttgcgg
     6661 tacgtgcgga tcatctcgac gatcggcccg ttgttgatcg cttcgagctt catcacctca
     6721 tccaagccga tgccgagcag aatcatcacg atcgcccgct tgggtctgcg ccccgattct
     6781 tcaaacgcct cggccgcttc ttcgggactg cagccggttg cttgctgaat caggcgcttg
     6841 gaccgctcca ccagtttcgc gttgaccgga gtaaggtcga ccatcaggtt tccatagacc
     6901 ttccccagcc ggatcatgct ggtggtggta agcatgttca acacgagctt ctgcgccgtt
     6961 ccggctttca tccgcgtcga accggtaagg acttccggtc cgacgtccag gaagatccga
     7021 tgatcggcgt ggaggaatgt tcttgagtgt tcattgcagc tgatcgcccc gaccaccgct
     7081 ccgatcgatc gggcccactt catcgccccg atcacatagg gggcttggcc gctggcggta
     7141 agaccgacga gcacgtcatc cttggtgaat cctcgcttga tgagctcgtc aatcccgtct
     7201 tcttcatggt cttccgcatt ctcgaccgcc cggacgagag cctcgcgacc gcctgcgatg
     7261 accccttgga ccatctcagg gctcactccg aaggtcggag ggcactcgga ggcatcgaga
     7321 acccccaatc ggccgctggt gcccgctccg atataaaaaa gtcttccgcc tttcctgaag
     7381 gcggcaacga tatcttcaat gagggtttcg attcgaggaa tctgggtttc aaccacatag
     7441 gggacgcttt tatcttcatc gttgatcaac tgaagcacct gcagcgtagt catctcatca
     7501 atgttggaag ttcggacatt acgctgctcg gtagtaagcg aatccagtgc atagtcgatc
     7561 ccatatttca tacgtttctc tcctcgcact cctaactgta tcatcgaccc aatccgttgt
     7621 aaagaaaaat tgtttcaata tttattgcgt tgtgaaatta tgtatcatat actgattcat
     7681 atgaaaaaga cctcgttgtt catcggcctc atgagcggca cttcgatgga tggaatcgat
     7741 gcggttctcg tcgaaataac cgaagaagat tccgcaatca aagcggtcaa gctgcttgat
     7801 ctcgcctcgg tcgaatatgc ccccgagctc aagaccctcc tgcttgggct ttgctcggct
     7861 cggatgatgg acatcgatgc gctcacccga gcccatttcg gactgggtga atggaacgcc
     7921 cgggccgttc ttcgactcct ttcaaaaacg aaggtggatc cgaaggagat cgccgcgatc
     7981 gggatgcacg ggcagacgat ctggcacgct ccaacaaaac taccctttcc cggaatcacc
     8041 catccttttg aaatcggcgg tacgcttcag atcggcaatc cccaggtcgt ctccctcaga
     8101 acggggattc cggtcatcag cgacttccgc gccgccgatg tcgcccttgg gggcgaagga
     8161 gctcctctgg ctctcatcat cgaccacctg ttcttcagtg aagcgggcaa gaccgttgcg
     8221 gtgcagaata tcgggggaat cggaaacgtg acggttctgc ctaaagcggg tgagctctat
     8281 gcgtttgaca ccggaccggg caacatgatc atcgatgcgc ttgttcagcg ccataccggc
     8341 cagccctatg atgacggcgg ctcgctcgct tcccggggaa agatcgatga agctttgttg
     8401 catcgcttga tggatgaccc ctacttcgcc ctttcacccc cgaaatcaac cggcagggag
     8461 gtctatggcg ctccctacgt tcagcgcctg atcgaaatgg cggaacacct cccgttcgaa
     8521 gacctcatcg ctacggccac cgcatttacc gcctggtcga tcgccgacag ctacaagcgc
     8581 ttcgtgatgc ctcggcacgg ccttgaccgg gtggttgtca gcggcggcgg agcccggaac
     8641 aagacgcttc tttcctacct tggctcctat ctcgaccgtg aaatagtgac cggcgaagcg
     8701 tatggtgttc ccgatcaggg acgggaagcg atggcgttcg cccttcttgc ccatgagtca
     8761 ttgaacaggc gaccgtccaa ctacccgctc gccaccggag cacgggaaag aacgatcctc
     8821 gggacgatca ccctgcagga gccggttcaa atctgatcac agcaccggac tgaagtcggc
     8881 gagcgggcct ccgatgagcg gcttgcacca ggaaaggtat gattcatcga cggttccgaa
     8941 ggaaggcaga tattccatcg gaagggtctt ttcgtggagc atcacctctt caagcgggat
     9001 gaggacgggc tcgacgcgat agggttcatt actgattcgc ttaaagccga ccatcacctc
     9061 gctgttgcct tccaaggccg cctgaacggc tttcctgccg acgagaatcg cttcgtcgcg
     9121 atcgacggag ctttgaaagg cgatcgagga acgcccgaga attcccggtt tttcacttcg
     9181 ggctttgact ccaaggcgct tgatcaccag catcgcaagg tgggcgctca catcgccgta
     9241 gtagaccgag cggtcggttt ggaagatcgg ctcgacgatc ggttcgccat tcgcagtgcg
     9301 cagtccctcg ctggccacga cgacaacatg ccgatgtttt ttgtagaggg aggagacttc
     9361 atccaggaag cgctcttcgt caaaagcgag ttcaggggca tacaggagat gggggccgta
     9421 gatgccttcg cgccttgcga taagcgcggc ggcggtcagc caacccgtat tccggcccat
     9481 cgattcgatc accgagacat gaatcggcaa agccgccaca tcggcggcaa gctcctgaac
     9541 ggaagaagca agatagcgag cggcgcttcc aaagccggga gcatgatcgg tgacggcgat
     9601 atcattgtcg atcgttttgg gaatgccgat taccctgatc ggacgatttc gcttttggat
     9661 ctcccgagcg atttttcccg cggtatccat cgacccgttc cctccattga agagcacggt
     9721 atctatttca ttggcgatga gaatgtcgac gatcctccga taggaagcgg catcgatgtg
     9781 atcgcggctg gttccgatca tcgagcctgg agtatgaggg aggcgttcaa tcgcttcctt
     9841 cggcagggaa agaaggtcga cgaatgcttc cttcaggatt ccccccgaac cgcctttggc
     9901 tccgtagatc ttcccgtacc gaccttcggc ttctttaatg acgccgtaca gcgaagcatt
     9961 gatgaccgca gtgggggctc cgccatgaac aaccaaaaga cgtttcttca ttcttctctc
    10021 cgataagcta ttttgcctga agtgatggtg gcgaccacat ccattgatga atccaagatc
    10081 gtcgcatcag cccgttttcc aagggcgatc tcccccaagt ccgacaatcc gagcattcgg
    10141 gcggcgttgg tggaaagaag cgggacgatc cgctgaagcg gggcccgggt aaaatcaaga
    10201 agatttctca gcgctttgtc catcgtcagc gtagaaccgg cccggacgcc gttctcgaca
    10261 agtttcgcat ccccgtcctt gacgatgacc cggttggggc caaggagata ctcgccgtcg
    10321 ggataccccg ccgccatgat cgaatccgta acggagatca tccgatccca cccttttatc
    10381 ttgagcaaca gccggacgat cggtggatgg aggtggaagc cgtcgacgat catctccgca
    10441 tagatgtcac gctctaggac agcggtgagg atcgccggat cgtgcatgtg gagcagcttc
    10501 atcgcattca tgatgtgggt ggtggaactc gctccggcgt cgatcgctcc gatcgtctcc
    10561 tcgtagccgg ctccgctgtg cccgaggctg actcggactc cccgtccggt caactctctg
    10621 atcaaggtcc tgcaaccccg tacctcaggg gaaagggtga tcactctgat catcccgcga
    10681 gctgcatcct gcagctcgtc aaaacaggaa aggtcgcact ccttcaggag ctcttccttc
    10741 atcgctccct tgtaggaggc gttgagaaac ggtccttcca gatgaatccc gagaatctgg
    10801 ggaaagcgtt tgagcaatgc cggtcgggca agcttggaaa gctgcgtttt catcacctcg
    10861 ggctcatcgg tgaggaccgt cggcagaaac ccgctcaccc ctttggaagc aaagaaaccg
    10921 acaaccttct ctatctcctg ttcatcggca aaaaggttga aatccacccc caccgccccg
    10981 tgggtatgga tatcgatcat tccggggatc acatgcatat tttccgcttc aaacacccct
    11041 ccggcgggac gattctgatg aaagccggtg aatgctccat cttcgatgaa cagatccagc
    11101 tcttcacatc ttccattccg gtagaccttt ccgcctctga tcagcaaaga ctcattcatc
    11161 ggccacctct ttgcagctct cccgccgaat cagcgtgaat gggagccgga cgctgatcgg
    11221 gaatgtccgc ttgctttcaa tccgatccaa aagagtcttg acggcaagcc ttccgagatc
    11281 ccgcttggga atatcgatcg tcgtcaacgc gggcttggtg aacgccgtcg cttcgatgtt
    11341 gtcgaagccg atgagcgaca gatcgttcgg aacgtggata cccgcttcat caagcgcttt
    11401 cagcaccccc atcgccacgg tgtcattgct gcagaagatc gcggtgggaa tctttccccg
    11461 gctgatgagc gttttcgtcg cttgatagcc tcccgtggcg gtcagaatcg tattgacgac
    11521 cagtgaatcg tcatattcga tccccgcctt cctcaaagcc cccagatatc cattgaaccg
    11581 atgctcgttg atcgtctcat ctttttgcgg gatgtgcccg atgaacccga tcgcccggtg
    11641 gccgagtgca agcagatgct ccaccgcttc ttttgtggcg ctttccgcat cgcagatcac
    11701 ttcatccact ccatttccca aacggttcac tcccgcatag agcaagatgg gaatgctccg
    11761 tttcagcctt tgaatcgtat cgatcgtcgt tcgtccgaga ataattgccc cgtcgaggtt
    11821 ggtcgtttcc aaaaaagcgg gaaagctcgg gtccttgacg tacagggtat gaaacgtcag
    11881 tgcgttgcgg gaactctggc ggaacacctc ttcctgaaca ccctccagaa gcgccgcaaa
    11941 gaacggggaa agataggttt catgatcgct ggtgaggatg caggcgatgg acagctgcat
    12001 cgtcgtcaag gctttcgaac gcatccgata tttctcaaaa taccctaatc gacccgctgc
    12061 ttcctggact ttggcgatcg cctcgtcact gaatgatcga ttggggtcat ttgagagaat
    12121 ccgggaaacc gtcgagatcg acagccctgt ctctttcgca atatctttta gtctggccac
    12181 acctttccct ttcccaactg atgatacatg ttcttcttat acccttccac cccatcctgt
    12241 gtgaagggat tcactccgag gagcagcgaa gagagatata cgctgaagag gaaaaaatag
    12301 aatgactgcc caagcgaata ggcggaaatc gattgcgagc tcaaaagctc gatcaccggt
    12361 cgatggtcgg aggcgtgagc gttcaaggcg gcttggtaga caacttcatt gagcgtatcg
    12421 aacggacgat catcgaggta gtcgaatcgg tcttgaatct cgcaagaagg ctcgatcacc
    12481 agcggcgatg atttataggt atagttgaga aacgtttcaa cgatgatgcg cggtccttcc
    12541 tgaacatatt gaccgatcgc atgaagatct tccgaatagg agaagtacgt cggaaacaga
    12601 gctccctcct tttttccttc gctttcgccg aaaagctgaa cccaccagcg ggcgaacccc
    12661 tccaaggccg gctcaaagat caccagcgct tcaatgatgc ttccctgttt actcagcaga
    12721 ttccggatcg ccgcatagcg aactgccgga ttcatcagcg gtttgagcgt cgtaagccgt
    12781 tgatgctcgg cgatcccgcc ggcgatcagg ttttcgatat ccacccctgc aaccgccatg
    12841 gggaacaggc ctaccgtgct caggacggaa taacgaccat tgatatcggc cggaaacgcc
    12901 aaaaactccc actgatgacg ctgagcaaga tggtggagct gaccttcgcc gcccgaaccg
    12961 atcgctatga ttcgttcatg ggaagaggat ccgtatttcc gttcgagctc tttgcgaagc
    13021 atccgaaatg tgattcccgg ctcgatcgtg ttgaaatcct tggcaatgac gcacagggct
    13081 gcgtttcgct cctgaaccaa gcggatcgct tttgccagct gtgtcgccga gagcgtatcc
    13141 ccggcaaaaa acagttctgt tcccgtcgga gaaagtgcgc caaccgccgc cacggcaccc
    13201 cggttcgaac cgccgattcc aatgacgatg agcagatcga accgtgcctg cacccgggcg
    13261 gcaaggcttt gtatcgcctg aagcgattgc attgaggtgt tgggatcgaa ccatcccaac
    13321 ggtccctcgg catacgcctc ggcggaaaga ccgctttgaa taagcccttc gttgtacaga
    13381 atgaaggagt cgacggcgcc ttctttgatt ccggaaatcg ttacttcaaa tgacacggca
    13441 tgctccatca acaagagatc cgcattggca aggcatctcc ctgccaatgc agatggttta
    13501 tccacttaca gggtggcgat cgccttgagg aggaacgtat cgttgaacgc ctcttcaaag
    13561 ctcttcacct tgtttcctgc gggaacgttt cctttagcca cgttgtacaa ccctttcagg
    13621 taatgatatc cggcgccatc cttattggca aaccaggctt ggagatcctt tttggaggga
    13681 gccacaaggt tgaacggatc aaacgtgcct tccggtttct gactcaatcg ctcgccgatt
    13741 ctcatcgcct catcgatgtt ttccgccctc cagaccgacg cttcatacaa cgcattgatg
    13801 aacgcctggg ctacttcggg attctcttcc atccacttgt tggaggcaac ccatgttgac
    13861 gggaaggatg ccgacggcgg gaagtgggaa acggtcgttg caaccaattc agtattgggg
    13921 ttggtccgaa tgatcgtgct ggtcgcagga gagaacccga cgacgatgtc catgccgccg
    13981 gcggcgcttg ccatgccggc cggaaggttg gagttcaggg tgttcaccac ttcgaccctg
    14041 ttggcggcgt tggtgttcgg tgccttgtat cctgcgatat aggaagcggt ttcgctgctg
    14101 atccacagct gctggttgac gggtcggcct tcgttgatca gattcaagag agttttaaac
    14161 cagccgccgg gggttgtacc gacttcgata tataccttct tccccttcag gccttgatag
    14221 atctcttcaa agtcatactt tccgtcaccg ttggcatcct tgaagacccc tttcttggcg
    14281 acaagcatct cggcgttcga gagacaatca agatatacga aggaaagctg gtttccggcc
    14341 ccgtcgatcg ggttccacgc gacaccggcg ccgatatatc caatatcaag ggtgcggttg
    14401 tcggcgcgca tcgcggtcat ctcggccggt ccgctttcaa cgatggtgaa aatcggattg
    14461 attccgtatt tttcaaagaa ccccttttcg gcggcgaccg ctgccagagg cgtgccgccc
    14521 tcattggcgt gaatgccaag gcggacgtct accagaccct tcttcccctc ctccttggct
    14581 ccggcggcga aaaccatcgt catcgccagt gtaataacga gcaaacttac taatgctttc
    14641 ttcatagcat tcctcctgtt gaaccacttc tgcggctcct tgtatcctta cttgggaatc
    14701 tccagataca tcctgtagac cgtatcccaa atataattct tgatctttat gaactcgctc
    14761 tccatctttg tcgcctgggt ccgcggatag ggcagatcga ccgggacgat ctccttgatc
    14821 tggccgggac gggcgctcat gatcaccacc cgtgatgaaa gcaggaccgc ctcgtcgacg
    14881 tcgtgggtga tgaagaagat cgtcttcttc tcgctctccc acgttcggag gagatcttcc
    14941 tgaagctgcg cccgggtctg cgcatccagc gctccgaagg gttcgtccat caagaggact
    15001 tcgctgttca gggcataccc gcgggcgatc gccacccgct gcttcattcc gccgctgagc
    15061 gctttcggat agctgtcctc gaagccggtg agattcacca tcttgatgta atgctcggca
    15121 agggcgcgtt tctcttcttt cgtatagcgg gaaccatcgg ctttcgtgat gaacttaagg
    15181 ccatattcga tgtttttccg gaccgtcatc caaggaaaga gcgcatattg ctgaaagatc
    15241 actccgcgct ccgggcccgg tccgatgata tcctccccgt gcatcgtgac gcttccgctg
    15301 tcaaatgttt ccaagccggc gatgatgttg agcaatgtgg tctttccgca tcccgaagga
    15361 ccgacgacgc agacaaactc attcttttcg atttcgaggt ccaccccatc gagggcgaca
    15421 acattcggtt tcttttcaac gataaactct tttcgaacgt ctttaatgat aatcaagggc
    15481 tttttcatcg tcatcctact tccactttgt caaattcttt tcaatgaaca acagaatctt
    15541 gtccaagata aaaccgataa ctccgatcgt gatgattccg agaagcacga tcgccgtatc
    15601 catgaactgc tgcgctccct gaatccgggc tccaagaccg aagatcgtcc cggtcaactc
    15661 agctgcgatc agggtcgtaa gcgccgaggc cattcccagg cgcgcaccga caaggatgaa
    15721 ggggaatgat gcaggaatga tgatgtcgat gaacagatcc cggtcctttg ccccgaaggt
    15781 gtacgcagcc ttgatcaggg tcttgtcgac ttccttcact ccctggtaaa tggtcaccac
    15841 catcaccaga aacgtggcga tgaagatgat cgtgtacttc ggcggttcgc cgagccccat
    15901 cgctaaaata accagcggga tcagcgcgat cgggggaatc gtccgcatga actggatcca
    15961 aggctcaatg agatttcgga ctttgggata ccaacccatc aaaaatgcca ccggaatcga
    16021 ggcgacaaac gccaaaccga agccgatgag aacccgttga agcgaaatgg agatgtcctt
    16081 ccagtatcga cctccggctt ttgactcgct gatcaaggct cgaagcacct gcttcgggct
    16141 tgccagaaag gtgttgatcg tggggatgag ggtaatgaga taccaaaaga aaaatccacc
    16201 aataattgaa accgtcatca gcgtgtagcg catccgggcg ctgccgtcgg agaaatcttt
    16261 caatttccgt gcgctctttt tttgttctgc catcttctcc ctccctgtca ccgggcatgc
    16321 atcttctgtg gatgtcttta aaataggcaa atatttgctc ttaaaaacca acttttaacc
    16381 tatctgactc cattattcca ccacattttt gatttgtaaa ggtttttttt catctttttt
    16441 ctctttacaa atccaaaaac cactcctaaa ctattatata tagcacgtcc ccgcaagaca
    16501 aaaatttgga taaaggatat gtcgatggct tctacgattt tcttaaacct taagcggttt
    16561 gatatttctt tcgaccgcgg cgggatcaat cgcctggccc caagcgctca atgggggcct
    16621 actctgggtt ccgctctcac cgggctgctc agctcttatt ccaaggaaac gcggtttgtc
    16681 gcatttcttc ccgaagctca cctcatcgga gccatccaag cttccgatgc cgatgttatc
    16741 tcgttcggat cccaatcggt ccactgggcg gacacggaaa tcggagggaa tttcggcgcc
    16801 ttcaccaccg agcggaccgc caatgcgatg aaagacctcg gatgtctctg gacgatgatc
    16861 ggacacagcg aagaacgccg cgatcttgcc cgtcttcttt cacttggcgg agcgaccgga
    16921 aaggagctgt tcggagctct ccaccggatt ctcaatgaaa aagtcctcgc tgcgcaaaaa
    16981 gcggggctga acgtgctgta ctgcatcggc gagaacgccg atgaagtcaa cattcgaaca
    17041 agcctcctgc aaaaccagat tgaagccggt ctcgccggaa ccgatcaatc gctggtcacc
    17101 ctcgcctatg aacccatctg ggcgatcgga ccgggaaaga ccccgcccac cgctgaagag
    17161 atcgcttcca tcgcaaaaga gatcaaatcg ttcacggata tggagctcgt ctatggcggc
    17221 ggcctgaagg aagagaatgc ggaggcgatc ggatcgatcg gttccttggc cgggggcctt
    17281 gtcgctctga cccgtttcag cggcgagatc ggattttacc ccgatgaatt tgcaactatc
    17341 gttgaaaact atacacgagg aaggaacatg tcatgaaatt agcgtttgaa tacggccatg
    17401 ggctgatgga agcgaatctg ccggatacca ccgacgtctt tattcccggt gaaacggtga
    17461 aggatcctcc cgtactggag gacatcgtcg gggcgaccag agccgcattg gagaatcccc
    17521 tgggaatgaa gccgttgagt cggctggtaa cgagggattc aaagatcgtc atcatttttc
    17581 ccgataaagt gaaagggggc ttccaagcgg attcccatcg aaaggtctcg attcccctga
    17641 tcatcgaaca atgcctggcc gccggagccg atgaacgaaa catcaaactg atctgctcaa
    17701 acggccttca ccggaaaaac accgatcggg agatcaggtc gatccttggc gatgcggtct
    17761 tcgaccgctt tcatccctgg gggcagatcg tcaaccacga cagcgaggat tgggacaacc
    17821 tgatcgatct tgggcatgag ccgcactaca actgcccggt gatcatgaac cgggaggtct
    17881 tcgaatcgga cgtcgccatt ttgatcgggc acgccctcgg aaacccctac ggcggctaca
    17941 gcggcggcta caagcactgt gcgaccggcc tcacccactg gaaatcgatc ggaagccacc
    18001 acaatccggc gacgatgcac aaacaggact tcgtgccggt gaacagtcat tcggagatgc
    18061 gcggcaagtt tgacgcgatc ggccggtaca tggaagagcg gatgggcaag ccctatttcg
    18121 tgtgtgatgc ggtccttgat accaacagcc gccagatcgg ggtgttcgcc ggaacggcgg
    18181 aagaggttca gaaggcgtcc tgggcgcttg ccgacaagcg gacctatgtg ccttgggcga
    18241 aaaagaagta cgatgtcatg gtgttcggga tgccgcagga gttccactac ggaaacggca
    18301 tgggcaccaa cccgatcttc atcctccagg cgatcagcgc ccagatcatc cgacacaaac
    18361 gcgttctggt ggacaacccg gtcatcatct gttcctcgct ctgcaacggc tacttccatg
    18421 atgaggagtt tccctcctat cgcgagacct ttgaactttt ccaaaaagat tatgccaaca
    18481 cccttcccga tatcgagcgt ttcggggagg cgttcagcat gcgtaaggag tacatcgacg
    18541 cgtatcgcta cagctacggg ttccacccct tccatgcgtt ctcgatgatc agctgcggcc
    18601 atatcgccga acagcatgcc agcgcaatct acatcgtcgg agcgatggag ccgggaatcg
    18661 cccgggcgat gggaatgaaa acccgaccga cgttcgaatc ggctttgagc gatgcggtga
    18721 agtacgtcgg caaagaaccc aacattctgg ccctgcctcg agcgttcaaa acagcgagcg
    18781 ttcatctgtg catggccgag ggaaaggagt gagcatgatc gacaaggcga tctatacaaa
    18841 agacaaaaca gcattgttgg acaatcccgg gattccgctc gcggtccttc ccacacagga
    18901 ggagacgttc cgctcgatgg cggatgacat ggtcgatacg atcagggaac gaaacgccgc
    18961 aggcaagcgg accgtcttca tcgtccccgt cggaccggtg gggcaatacc cctacttcgt
    19021 acagcgggtg aatgaagagc gcatatcact gaaaaatgta tggtttttca acatggatga
    19081 gtaccttgat gagcatgacc ggccgatcga tttcgacagt catctgagct tccgcgggtt
    19141 tatgcaccgc gaggtatacc aaaagatccg gaaagagctc gtgatggata ccaagcagcg
    19201 gatcttcccg gatccggatc accctcgtct cctcaccgaa accctcgaac ggcttggcgg
    19261 ggcggatgtc tgctacggag ggctcgggat caacggtcac gtcgcgttca acgagcctga
    19321 tgagatttcg gtagaggagt tcaaggaact caagactcgg gtggcggaca tcgctatgga
    19381 cacgcagata gcgaatgcga tcggttcgtt gaacggagcg ctggatgcga tgcccaggcg
    19441 atcggtaacg atcggattca atgagattta cgcttcaaga aaaatccggc tttactgctt
    19501 ccgtgattgg catcgcgcag tggttcgcca tgccgcccac ggtccgattt caacccgttt
    19561 tccggtaacg atcttgcagg agcatcctga ttgccgaatc acgatgagca gcaatgtcgc
    19621 cgaaccttgt tggtagagtt ccggtccttt ctttgatagg atacgtttta gaaaggagtc
    19681 aatcatgaga aaggcgttat gttttggttc attgaatatt gactacgtct atgacgtacc
    19741 gcatattctt cgcccgggag agacgctcgc ttcgacgaat cgatcgatct atccaggcgg
    19801 aaagggcctg aatcagtcga ttgccctcgc aaaagcggga gtcgccacct ttcacgccgg
    19861 cgccgtcggc gaagggggcg agctgctgct tgagacgctt caggacagtg gagtggatac
    19921 ccgattcgtc cgaacaattg aaggattgtc cgggcacacc gtcatccagc gcgatgcaaa
    19981 cggacagaac aacatcatcc tgtacggagg ctcgaatcag gcgatcaccg aagagcagat
    20041 cgatgaaacg cttcaatact tttcttccgg tgattatctc ctccttcaaa acgagatcaa
    20101 cctcatcccc tcgatcatga ccaaagcggc ggaaatcgga atggtcatcg tgctgaaccc
    20161 ctccccgatc gatgaaaaac tgcttgccta tcccttggag ctggtcgaca tcttccttct
    20221 gaacgaaatc gaggcgatgg atatcgtcgg atccgaatca ccccccgatg agctgcttcg
    20281 ctccctttct tcaaaatttc ccgaagcgca gatcgttctc accctcggtg aggagggggc
    20341 tctttatctt gaccgcaaag gtggaaagat tcttaaacac ggaatcttcg atgtggaggt
    20401 cgtggatacc actgcggcgg gagacacgtt caccggctac ttcattgcga ccttggccaa
    20461 aacgaacgat cctcaagaag ccctcagggt ggcttcgctt gcgtctgctt tggtggtgag
    20521 ccgcttggga gcggccccct cgatccctac aaaggaggag gtgtattcga tacatatata
    20581 tcttaaagag taaaaagcct atttaccttt tgcctttcaa gtgagtatgc ttctttaaaa
    20641 acggaggtag caatggagag tacatacatc tatgttcccg gcctttggct gtacatcgtc
    20701 agtacggttg tcctcctcat gttcatgacg gcccaagttc cccgcatcca cctgcccctg
    20761 tcaaagcgca tcttctgggt tctgttcacc gcttttctgt ggaacttctt tctcatcctc
    20821 gagttcatgg tctcgacgat cgaactgaaa ctcctttacg ccaagttcca atacatcggc
    20881 atcgtctggt tgccggtcac ttggcttgct cttgtcgtgg agctctcatc ctaccgattc
    20941 agcaaacgga tcttcgccgt ctcgtccatt cctcccgtca tcttcctcat cttcattttt
    21001 cttgttcctc aaccgaacgc cttctggggg attcccacgc ttgaggacac ctcactcttc
    21061 ccaatggtga acttccacta cggcccgctc tattatttcg gctttcttcc cttcagttat
    21121 ctgatggctt tggcctcgat cgtcgttctt tggcggaact atggaaccga ccacgtccac
    21181 tatgtgcgcc agaaactcct gctcaccttc gtcctccttc ttccgggtct gaccaatctg
    21241 ctgtacaatt tcaatctcag tccggtcagg ggcctgaacc tttcaacggc cactctgacg
    21301 gtcagcggcg ttctgttcta catcatcctc taccgcaact cgctgttgga cctgctgccc
    21361 gtcacccgcg aacagatcgt tgaaaatctg gacgaagcgg tcttgatctt caacccgcag
    21421 aagcgactga tcgatttcaa ccattcggcg gcgaagctgt tcaatttggg ccgcaactcg
    21481 atcgggacag gagtcgaaga aatcggagtt aaagagctga ctcaccgact tgaaaatctt
    21541 caggacgcgg agtccaagac cctcgccctc atgcaaggtc tggtctacga tctcagtctc
    21601 aatttcgtcc gggatccgag ctctgaattc tgccaggcga tcattgtgac gcttcgtgac
    21661 atcactcagg aacaactgat gaatcaacgg cttcaaatcc ttgccgaacg ggactcgctc
    21721 accgggttat acaaccgcca tacctttttc ttccggagca aggaactgat caagaccaac
    21781 ccgagagtgc gcatcgccgc aatcatgatc gatcttgaca acttcaaagc gataaacgac
    21841 acctacggcc acgcctacgg tgacttgatt cttcaaacga tgggggaagc gctccttgcc
    21901 ctgacctcgc caaccatcgt tgccggacgg ctcggcggtg atgagtttgt cgtcatgaca
    21961 acggaacagg atctgtcgga tcttgcctca cgcctgtcga aaaaggtcta cgatcgactg
    22021 aatgtcgtgg tgaaagaagg gctgaacccg actctttcag tcggagtcag ctccctcaat
    22081 cttgcatcat gtaccgtaga ggatcccctt gaatatctgc ttaaccaagc ggatctggcg
    22141 atgtaccaag ccaaacgagg gggaaaggac aagatagtct ttacagggga cttcattccc
    22201 ggctagcgct tccattgctc gttttgtcgg gttcctcgaa tgcggtgttc ggcgccatat
    22261 cctcccctga taattaggat ggtttatccc tcatgaatgt tgcttgtttt ttcgtatttt
    22321 cttgaatttg ttgcaatctg ttgtattatg aagggaggaa aaagagcact ctgtcttccg
    22381 gccgcttcgg ttctttgcat gctcatcaca tgatggatga cagagtgcag ggagggttgc
    22441 atgaagatta ttggttcttc acctaatcga gtcgatgcgt ttgacaaagt aaccggtaaa
    22501 gcgaaatatg ctcccgatct ccttccacct catgcccttg aagcgaaagt gcttcactcc
    22561 acgattgcca acggctgggt cagagagttc gatctcagcg aggcgtggaa ggttgaaggg
    22621 gtcgtagaca ttgttacctg ctttgatgta ccggatattc aatatcctac cgccggccac
    22681 ccttggtcaa atgtcccggc caaacaggat atctgtgatc gcaagctgct caacacccga
    22741 gtccgccagt acgccgatga tattgctgcg gtgatcgccg agacggagct cgccgccatt
    22801 gaagccgtca ggaagatcaa ggtcgtttac gaggagcttc cggtgattct ggatgtaaag
    22861 gaatcccttg aaaacaccga atttcccctc catgaggagc gaccggacaa tatcgtcaac
    22921 aagacctcct acgagatcgg ggattttgaa gaggcgatca aggaaaaggg gttgaccaga
    22981 atttccggca cctacgagac cccggtggtc tcccactgcc acatcgagcc ggcaaactcg
    23041 ttcgcctacg aagaagcggg aaaaatcgtc gtcgtcagcg cgacgcagat tccccacctc
    23101 gtccgaagga tggtcgggca agccctcgga gtcgattggg gaaaggttcg ggtaatcaaa
    23161 ccgtatatcg gaggcggatt cggaaaccgc caggacgctc ttaccgaacc gctgaacgcc
    23221 tacctcacca ctcgggtggg aggacggccg gttcggctcg cctatagcag ggaggaaacg
    23281 ttcgagtgca ccagaacccg ccatgcgatg accttcgacc tgaccaccta tgtccggaaa
    23341 gacggttcct tcgccgcccg tgatttgacg gtctactcca accagggcgg ctacgcctcc
    23401 cacggccatg cccttgcggc gaacgcggcg aacgccttca ggatgatgta tccggtcgga
    23461 gcgatccgcg gcaaagcgat caccgtctat accaacatgc cgaccgccgg agcgatgaga
    23521 gcatacggaa ttcctcaaat cgccttcgcc ttggagtcgc acattgatga catcggcaaa
    23581 gctacgggac tcgatcccct cctgcttcgg acgaagaaca tgatgcccct cggctatgtg
    23641 gatggaccga caacgatcac ctgccactcc accggacttg ccgagtgcat cgccaaaggc
    23701 gaggagcatc tggattattc gaagaagcgc ctcgcctacg ccaatcagga tggtccgatc
    23761 cgccgaggcg tcggcatggc gatcttctgc tataagaccg gcgtctatcc gatcagcctc
    23821 gagacctccg cggtccggat gatcctcaac caggacggat cgatgcaagt acaggtcgga
    23881 gcgaccgaga tcggccaggg agccgatacg atgtgggtga tgatgaccgc cgaaacggtg
    23941 ggcgtcacct tggataaagt gtaccttgtc agcaaacagg atacggatgt gactccctat
    24001 gataccggag catacggatc gagacagaca tacgtatcgg gaatggcggt gaagaaaacc
    24061 gccgaatcga tgcggcgcaa gattcttgaa tacgccgccc tgatgacgaa caaggatccg
    24121 atggatctcg atatcgtcgc caacaagatt gtgcggaaga ataccgacat cgtcctgacc
    24181 accgtcaagg atgttgcgat ggagtcgtgt tacagcttga cgaactccaa tcacatcgcc
    24241 gtcgaggaaa cccatcactg cagcgacaac acctacgcat tcggagtctg cttcacggaa
    24301 atcgaggttg atattcccct ctgtcaaatc aaggttctcg atatcatcaa cgtccatgac
    24361 agcggacgga tcatcaatca gacgaccgcc cgcggtcagg ttcatggcgg aatgagcatg
    24421 ggtctggggt acgccatgta tgagtaccac aagttcgacc ccaaaacggg ccggatgctc
    24481 aacggcaacc tcctcgatta caagctgatg acgccgttgg atactccgac cctccatgcg
    24541 gcattcgtgg aaacggatga cccgaccggt ccctacggca acaagagcct gggcgagcct
    24601 ccgacgattc ccgtcgcccc ggcgatcaga aacgccgtgc tcaatgcgac gggggtttcg
    24661 atcaacgcaa accccctcca ccccgaacgg ctcttcgact cgttcgtcga agcggggctg
    24721 atcaagcaaa ggagtgagca tgtataactt caaaaccctc tacgaagcga catccgtcgc
    24781 tgaagccctc aagctcaaag cggatcaccc cgaggcgctc tttcttgccg gcggcagtga
    24841 tattctcata aaaatccgcg aaggccgcct tgacggagtc gatctgatca acatctacat
    24901 gctggatgaa ctgaggggga tctgcctaga ggaggacgga tcgatcctca tccgcccgct
    24961 caccagtttc accgatgtgg cgatgcaccc ggtgatcaag gagcacctgc cggttctcgg
    25021 cgaagcggtc aacacgatcg gcggacctca ggtgcggaac atcgggacga tcggggggaa
    25081 catctgcaac ggcgtcccct ccgccgactc ggcgatcacg ctgaaggcgt atgatgcgac
    25141 cttggagatc gtttcgacgg aaggaacgag aatcctgccg tatgcggact tcaacctcgg
    25201 tttcggaaag gtcgatctcg gtcccaccga acttctggcg ggcattcgaa ttcccaaagc
    25261 gagctgggaa aacaccttcg gccattacat caaatactca atgcgcaaag cgatggatat
    25321 cgccaccctc gggtgttcgg tgaacgtgaa gctttcaaag gacaagcaag cgatcgaccg
    25381 gctgcgcatc gccttcggag tcgccgcacc ggttccggtc cgctcgccca aagcggagga
    25441 gcttgccaag ggcaagaaca taacccaagc tttgatcgat cagatcggtg aggcggtgct
    25501 cggagacgtg aacccccgct cctcatggcg ggccggccgg gagttccgcc tgcagctcat
    25561 cgaagaattg gcgaagcggg ccaccatcga agcaatcaac aaagcaggag gaacgatcaa
    25621 tgtataaccg aagtgtaacc tgcaccgtga acggaaaaaa gcgaaccttc atgatcgata
    25681 tccgctcatc gctcagcgac atgctccgcg gcgaagcggg gctcgattcg atcaagcatg
    25741 gatgcgacgt cggcgaatgc ggggcgtgca ccgtcctcat tgacggcaaa ccgttcaact
    25801 cctgcatcta tctggcgatc tgggcggaag gaaagacgat cctcactttg gaggggctct
    25861 cgaccacaaa gggagcgatc agcgacattc agcaggcgtt catcgatgaa ggggcggttc
    25921 agtgcggaat ctgcaccccg ggcttcatca tggcttcgat tccgatcatc gaaagcgatg
    25981 taccggccac ccgcgacgag atcaaacggc agatcgccgg aaacctctgc cgctgcaccg
    26041 ggtatgaaca catcgtcgat gcgatcgaaa aaacgcagaa aaaacggatt gcggagcgca
    26101 cccgctagaa ctaacttggt cgttcggcta cactacgggt catgaatata ttgtcaaaca
    26161 ttcctgcctc agtgcaaccg tacgtaccgg cgctcggcgt cctgtttctt gccacgatcg
    26221 tcctgttggt cacccgggcg attctcaagc atcgaatcaa agtactcaat gctcgggaac
    26281 ccaaaccccg atacctcacc gtcttggaac tggttcacaa gattctgctt ccggtgttgt
    26341 tccttgttac cctctcgttc gcgctcaccc ttacgcaatt cagcacgacg gtgcgaaaga
    26401 tcatcaacat cagctttctg atgatcggaa cctacatcgc gatcagggcg atcaaccnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac ctcttcagct acttcgtcat
    26581 cctcttcgac catcccttcg gaatcggcga ttacatcgtc tttgatgata aaagcggaac
    26641 ggttgaaaaa atcggggtga aatccaccca gatccgggtc aagaacggcg agatcctggt
    26701 cgtcgcgaac aaagatctta ccgccagccg aatccacaac tacaagcgga tggaaaagcg
    26761 gcttcttgtg gctacccata acttcccctt tgacactccg tatgaaaagc tggagaagat
    26821 tccttccctg attgcacaag tcgtctcctc gctcacgacc tttgaaggca tcacgctgga
    26881 tcgatcccac ttcaaaacga tcggaaaagc gacatatgaa tttgaaagtg cattttatgt
    26941 gccttctccc ggctatatcg acttgttgaa tgttcaacag gaaatgaact ataagctggt
    27001 aaagatcttt gaagaggaag gaatccgctt cgccattccc acctacaagc ttcaggaaga
    27061 ttgattcgtt ttatgatcat ctcctatctg aggggctgtc gcaaaagtcg caaggctggc
    27121 ggcagctcct tttttcacta ttcactggag cgagcggacg aggtatgcga gaatagaata
    27181 tatccttacc agaaaggagt gcacgatgag ttcgctgttt ctttcccatg ccatctggca
    27241 acatccgcaa gtaacgagtc tgaaccgcct tcccatgacc tcgctgcccc tgttctttcc
    27301 gacgttggaa gaggctctct ccgatgcgct cggaggaccg gaaaagcgag atctttcaga
    27361 tcatccctat cataagagtc tgaaaggaac atgggatttc atcctttacg acaatcccct
    27421 cagtgttgat gaacgcgtgc tcgatgagaa gaattccctt tcgtggaaac cggtcatcgt
    27481 tcccggttcc tggagcatcc aaggctttga caaaccccac tacaccaacg tcatcatgcc
    27541 cttcgaggat gatccgcccc atcctcccca agtgaatccc accggtgtct atcgcaccgc
    27601 gttcaccgtt gacaatcatt ggaaaaaccg aagaacggtc ttggaggtcg gcagtgcgga
    27661 aagctacgtc gagctctacc tcaacggagc cttcgtcggc ttgagcaagg acacccgcct
    27721 cgaagcctgc ttcgatctca ccgattactt gaaggaaggc agcaacactc ttgttctcat
    27781 cgtcgttcgc tacagcgaca gttcctacat cgaagatcag gatcagtggt ggttcggggg
    27841 aatccatcgt gatgtaaccc ttacatccct ccccgagaaa acccttagtg acatcaaagt
    27901 gactccgacg ctcaatgaag ccctgaccga aggaatcatc gagatacgca cccccggaaa
    27961 tcaggtgatc gaggccttgg atgtgcgagt gtacaccgat gcaggaaaac tcatcgagca
    28021 ggaaattttg aatgccgagg aaggagtgat cgtcggttcc ttgaaggtcg cttcaccgaa
    28081 gctgtggagc agcgaaacgc ctaacctcta ttacctggga atctcttctc tcaatgagca
    28141 tctggtcatt cccatcggct tcagaacaat cgagcttcat cggggaaatc tgctcatcaa
    28201 caaaaagcgg gttctcatca aaggagtaaa ccgccatgaa catgatgagc gaaccgccaa
    28261 gacgatcagc gtcgaatcga tggtccacga tatcaagctg atgaaacagc acaacttcaa
    28321 tgcggtcaga accgcccact atcccaacga tccaaggtgg tacgagctgt gcaaccggta
    28381 cggcctgtac atcatcgatg aggcgaatat cgagagccat tcaaactatg acgcgctctg
    28441 ccgggaggaa tcatacgctg cggcgttcct ggatcgagta cagcggatgg ttcgccgcga
    28501 ctttcatcac ccgtgcatca tcggctggtc gctcggcaat gaagcgggat acggagaaaa
    28561 ccacgatgct gcagcggcat ggatccgccg ctatgatccc ctcagggtca ttcactatga
    28621 aggggcggtc cgtcccgaat ggggacaagg agcccacacc cttgaaagct tgaaacgcgg
    28681 atcgttcgcc acggatctcg tctgcccgat gtatcccccg atagccttga tcgaagagtg
    28741 ggaccggacc accgatgcgg accgggatcc ccgccccttg gtcatgtcgg aatacagcca
    28801 cgcgatgggg aactccaacg gctccttgag tgattactgg gatctcatcc gctcttcccg
    28861 ttcgattcag ggcgggttca tctgggattg ggtcgaccag ggaatcctgg tcgatgacga
    28921 gggcaagccc gttggcttta gaaacgtcga ggaacatgtt ccgaaacgat ggcgctacgg
    28981 cggagatttc ggcgatcaac cgacgaatta cgatttctgc ctcaacggaa tcgtcttccc
    29041 cgaccggagc ctcaagccgg tgatgagtga atgtctgaag gttcaccaac cgattcacgt
    29101 gaggagcagc aatccatcaa gcggatattt cacgatcatc agcgagtatg atttcatcac
    29161 cacgaaggat atcgctctga cgtgctcgat ctacaatgag tcctcaagcg aggagtacac
    29221 gtttggagtg gagcttccca tccttcagcc gggcggccag gcacaaatcc acatcagaga
    29281 attgcaagag agccgagtgc ggaagatgat gggatcgtcc acgacgttca tccggttcgt
    29341 ctgcacattg aaggagcctg ctccttgggc gccgaaagac catatcgtcg cacttgagca
    29401 gtttgtcctc agcacgattt caaagtcgct tcacatccac gcccctgcgt atccgataaa
    29461 gcagaccgat gactcgttgt ttgtggaaac gattctctac aaagcttcga tcagcagtga
    29521 gggcttcctc tccgcccttc attttgaaga tcggccgccg ctgttgacat caccgctgag
    29581 accgagcctc tatcgggttc cgacgcaaaa tgacggactg aagacgtaca cccatcttga
    29641 agggataaag gagttttcct tctaccacga gaaaaaagcg atgaagccct ggttggagca
    29701 ctctttgaac gatttggtct taatcctgga agaactgaag atcagacaca atcaggtgaa
    29761 gacgattcat cagctcaaaa ctcataccgg cttggatata gggtctgttc gtcagacgtt
    29821 cacgttcacc ctcgaagaag tgtttttgca tgcgatcttt gatctgagcg atgcgttcgc
    29881 tgaatatcca aaagtcggct accgctgttc gctcacgaaa caagaggagc cggtttcgtg
    29941 gtttggccgg ggaccggagg agaactatcc cgatcgaaag accggttcgc tcatagcctc
    30001 ctacgacaag accgtcgaag agctcttcgt cccctacatc gttccccagg attgcggggt
    30061 ccgaacggaa acgacccgtc tggttcttga aggggcgaag atcgaaggaa tccttccctt
    30121 ctccttcagc atccatccct acagtcctga ggagctatgg gagaagagac acgccgatga
    30181 cctcgtcgtc gatgaggtcg accacctgag cctcgatgcg gcggtccgag gggtaggaac
    30241 ggcgacctgc ggacccgata cgcttgaccg ataccggatc ccaggcggca tctatcagat
    30301 gagattgcgg ctctcgtagg agacaacagc tcttattctt attcccatcg agggaaagaa
    30361 tactctcccc ggtagttcga cgcgttatac gttgaatatt ttacaggatg ataagaatac
    30421 tctcgttcgg gttgctctcg atcctgagta tacacccaga gttctttttg atcccagacg
    30481 atgatttcat cacgactgtc gccgaggatg ttcaatactt cacaacagag ttcaggatgt
    30541 ccgtcgtcgg ggaaggtgac gacgatgtcc ccatccccgt ccatcaggcc tccgtgctcg
    30601 acaccggaat gaatcaagag cagatcgcga ccggagccat cccagttcac cggtgtaata
    30661 agagcgccgt tgctccgcat ctcctttgac cagatctcct cacctttaca cgtatgcata
    30721 tagagaattc cctgagctcc ccaataggtc gttgtacaaa tctccagtcc tcgattttca
    30781 gggatataat tcccgacact gattcgttga ccatggccgt taatcgtccg tttgaggatt
    30841 gttccatccg gcttgagaag tatgaaccct tcccacccgg aaacgatcgc gataaggtct
    30901 tcctggtcag gatcaatcgg acccacgatt atctcatcgg tgtggtcgga atcgatcggc
    30961 agcttccaca tcagcttccc ttcgctgtcg atcatgttgt agcagctgaa gatctcatcc
    31021 tttccgtcat tattgaaatc acgggcgtac gggaaatgcc cggtattgtt gtgatggaat
    31081 ttccaaataa gatttaattc atcgtcaaac acccacaacc ttgcataacg gtctttgatc
    31141 aggatgtcgc tcggtctaac cttcccggtt acgttcacga tccggatcgc atcgacattg
    31201 agacgatcaa aggcatgatg cccgaactct attccatcga gttcttcaac cggttcgacg
    31261 ttgtaaggag ttggaatccg tcgttttacc gaaccgtcac gcccgtcaag gatatacaga
    31321 gtgaaatccc atgaagcgat cacttcatca atcccatccc catcaatgtc atacacttgg
    31381 aatggaagat cggtggtcaa tttaatcact ccatcatccg tacgaggctt gccaagctgc
    31441 caaagcacgt ttccgctctc aagactcacc gcagtaaggc aactgatcat cggataacga
    31501 tccttgtaca ctcttctttg actctgtgcc atcacgaaga agtattcttt cgttccggtg
    31561 agatgaccaa atcgaatctg tctccccgaa ccgaagtcct gcaaatcaat cttctttgca
    31621 acggcaagct ttggctggtt tgcccgtttc tgagcgagct tccgcctctt ctcgatccgt
    31681 tcctcttcta gacgtagcgc ttctttctcg gaggtcatga cctcaacatg cgcgtattgg
    31741 gtgggcatac accctgaaag tgcgatagca ccacggtcat accaatcgtc aaaaacctca
    31801 agaagccatt cgccgttgag tgtcaccgtg atggtatttc cgtgcacgga aacaatcaga
    31861 tcctgaaact cgtcggtaag ccaaggagcg gacgctttag ccagcaccgt tcgctcggtg
    31921 agatcgactt tgtgaacttc gattccgtca tccgtgaaga acactccata atggcggaga
    31981 ctcgtctggt agcggaccaa cactccgctg aacagttctc gggaaagggg gcgaagagcc
    32041 accttcagct gataatcctt ccaatcacga tcgccggctg ttaatgtcgg caacacatct
    32101 ctttctttcg gctcctgaat ccgtgatagc tccatcacgt tcacaccgtc aagtcgaggg
    32161 tttgttacta tccatgatgg gccggtatag ttccagttga cgatcggatc ataccagaag
    32221 cctttatatc cttgcgcggg atagtagtgg tattccccca tcgccgaatg ctcggggtca
    32281 taggggaagc cacctagatc aaatgaagag aagtcttcag aaaagagcgt cacattctgc
    32341 acaggaacaa tccctcattc attaccaaac gagcaatgaa tcaacatcga tcgccagtcc
    32401 gcttgcgatt gatttgttcg cagcgattcc ggtgagaatc gaccggagtc cggcggtatg
    32461 atcggcgctt cgcttgaggg gatcatgcgg cgggttttcc agaaaaatat cgtccagcat
    32521 cgccggatcg ccgcccccgt ggccgccttc ccgtacctcc acctcgacat catacgccgc
    32581 ttcaaaaaga ggaagaacct tgatatggtg gtagaccatc gccccttcat cactcttctt
    32641 tcctccggcg ttgatgtacg gcttctccat cgccgaatat tcgattcgac ctttgctgcc
    32701 gttgatcatc acgttgtacc cttcccacgg catgtaggag ttcaaagaat aggtcaggat
    32761 agcaccgttc ctgtatttca cgaggacccc catcgtatct tcgatggaga tctcgtcact
    32821 gaacacattg cgatccctga tatagccgct ttcctcttcc gcatcgagat acagtccctt
    32881 cagaacggga ctgctctcca agtcgattgc aaacggatca tccttcgcat agggtgaatt
    32941 gtggtaacga taggaaaagt tcgtcactcc ccgcttcact gctgccgctt gaccatagaa
    33001 attcaacgca cccatcgcaa aaaccttttt aggctgggtg tccatccaga agttgaccaa
    33061 gtcaaagtga tgggtcgatt tatgaaccag caatcccccg ctgttcactt tattgcggtg
    33121 ccagcggcgg taataatccg ctccgtgttc ggtattcagc agccactcga agtggattga
    33181 atacacctcc ccgaccgctc cgtcccggat aagctcccga atcttggagt ggtggggagc
    33241 atagcggtag ttgaaggtga cccgaatcga tcgtccggtc cgcttttgag cgtccagaat
    33301 cgcctgtgcc ttcacttcat cggtggtgat gggcttttcg gtcagcacgt cacacccttt
    33361 ttccatcgcc cgaatgatgt agtaatcatg agtgcgatcc acggtggtga cgatgaccac
    33421 atcgggcttc atctcgtcga tcatccgatc gaactccgtg tgcttatacg ttttgacggc
    33481 aggatggtcg cattcgtcag tgaggatcct gttggaaaaa tccattcgaa cctgactcat
    33541 atcacaaaac gctacgagtt cgcactcttg gcgataaact gtggcgatcg cctcatagaa
    33601 cattcgggcc cggccgccgg tccccacttg gacataccgt ttctttacac tcattccatc
    33661 ctccatgtgt ttaataagta tataggtcca ttcgaaacct gtcgttggca agaaactgaa
    33721 agttgttatc cttttccgta tattaataat cagtacgggt tgtgtgccga acctttcgga
    33781 tatattcgga tggggatacc cctccgtggc gtttgaaaaa tcttgaaaag tactgaaggt
    33841 tctcaaatcc aagtttccat ccgatctcac tgagggtgag atcggtgtct tgaatcaagg
    33901 aaagcgaacg ttcgaatttc atcaaattga tgtatttgat tgcagacaaa cctgtttgtt
    33961 tcttgatcac ccgatacagg tagtcttgat tgtaaccaca ctcatcggcc atttctccaa
    34021 tcgtaaatga gtcgccgaga tgattgttta tatatgcatc aacagcatca agtacatcat
    34081 tcttggttgt cagagagccg cttcttagct gatggattgg atttgaatcg tacaccggaa
    34141 gcacttgctc aagacagatc ctgttcatgg taagaagcgt ctccatcaat agaaccgatg
    34201 acatttgttt ataatgcacc attttacgtt gaccttccag aacaatgcgg cttaacagaa
    34261 gatagagctg attctcccga tcacgaaatc tagtatttat cccttccaag acatccttgg
    34321 gtttcctgct ctcggtcgtg aacttcactt caagcatctt caccccatcc ttgctggtcg
    34381 catgaaaggc atggtagcac ccttttttac aaaacacgat gtcgccggtt tcaagatcca
    34441 tcgcttcact attgaaatag tttcgtgccg ttccgctgta aacgagttgg atctgataat
    34501 catcagggtg atgatgctcc accattccct ggccctcttt ataatagaat cgtcccgatg
    34561 aggttattcg tacagacatg cgattcctcc cctttcctat tcactcgtca cagcacatca
    34621 tatgtcaaca accgattaat cataacaata ccgtatctac gaaaaagtat aaaccttttc
    34681 cattttctgc taacgactga tgaacaaacc cggccctata ctattcttag ttacctctcg
    34741 tacgagagat gtttccaata ggaggaatac acatgaaaaa gactaaacgc ttgctagtaa
    34801 ctctttccat tctcctgatc gcccttgttg ctcttgctgc agcaggtcag aaagatagca
    34861 agagttcttc catcccgacg atcacactgg ctacggcgga caacacctat ggtctgagca
    34921 cggaccctga attgcaaggt gccatcaccg ctcttctcga atcgaaaaca ggaacgaaga
    34981 tcaatccaat catcccgccg cttgcatcct acaccgacaa gctggccacg ctcatcaaca
    35041 gcggtgatat tccggatctg ttcgtcatcg ctcaggccat gagcaaagtt ccgacgatgg
    35101 tcgcccgcgg gcagcttctc gagttgaccg attacatcaa aaagagtccg gctctttccg
    35161 ccctcgatcc aaaccttttc aaagatttgc agatcgacgg aaagatctat ttcgttcctt
    35221 acaactatcc caaaagcaaa gccctcttcc ttcgtaaaga cctgatggag caatatgggg
    35281 tcaacctctc ctccaccccc accaccgaag agttccgccg tgagatggcg aaattcgtcg
    35341 gtacgggagt cgttcctttc tgtttcccca aatgggtcga caacttccaa tacttctaca
    35401 attcctttgg cgcttggggc ggcgtatatc taaaggacgg aaaatatatc gacgggttcc
    35461 aaactccgga gatggaagcg gcgctgagct atcttcgcca attgtatgtt gacggtatat
    35521 tgaatcagga gttcctgact accgaaaaca gccatatgcg tgaaaagaca tacacggctc
    35581 aagctgcagc atcgatcgac tacgtgacca actacgtcaa ctatgtgcaa aataccacgg
    35641 cagcgaacaa gccgaccgag atgcatctta tctacaaggt aattgggccg aacggcgacg
    35701 gtggaagctt gaatgaagcc acccagaccg cgctccttgt ttcagctcgg acaccgtatc
    35761 ccgatgcggc ggtaaaggtc ctcgaaacga tcgttaccga tcccgaggta tatcctgcgt
    35821 tctttggcat cggcctcgag ggtaaacact atacactcaa tgccgatcgt gaaatcgtac
    35881 ccacgactca agctgcaaac agcggataca agtacacctt aaactatctt agcgattcgt
    35941 ttatcgacat agatatcaac aatcttccat tcgctttaac caagaatcta caacaggctc
    36001 ttcccgctca gatcgggcat atcaagacga tggcaggcca tcttggacca aaccacgcgg
    36061 cggacgtgcc ggtaggtatc agcgatgcgt atgaccgggt tgttccttcg atcaagagta
    36121 cccgtgaatc gatcgcggcg aaaatcatcg tcggatcggt tacgctccag caaggaatgg
    36181 ccgagtacga aaatttctgg aagtcgatta acggaccgaa gattcttgaa gagttgaatg
    36241 cctcactcta agggctcaaa agcaccaaag caaatcggtt tccagaggct tttctggaaa
    36301 ccgattcttt aaaaggaaca attgtacgat gacaccgcaa caacgagctg aacacctagt
    36361 agaaaacatg agcatcgagg agctcttgag ccaattgcga tacaatgccc catcaatccc
    36421 tcgcctgggg attccagagt acaattggtg gaatgagggt cttcatggag cagccagaag
    36481 cggaacggca accgtatttc ctcaagcaat aggtttggcg gctcttttcg atccggtctt
    36541 tttgtatgaa atagcttcaa tcatctcgac cgaacagcga gtaaagtaca acctctacac
    36601 aaaggaggat gatcggggaa tctataaagg actgaccgtc tggtcgccaa atatcaatat
    36661 tttccgggat ccccgctggg gacgaggaca ggagacatat ggtgaagacc cctacctgac
    36721 ctccattctt gccgtcgcgt tcatcaaggg tcttcaaggc gaaggtcctc atctcaagac
    36781 cgcctcctgt gtaaagcatt ttgcagctca cagtggtccg gagccactcc gccatcactt
    36841 caatgcgatc gtctcggaca aagatctgga agaaacatat cttccggcat ttgaagccgc
    36901 cgtcatcgag ggacgggtaa acgcggtcat gggcgcatat agcgccgtga acggagaggc
    36961 atgttgcgcc agccccttcc tgattaaaga aacccttcgc ggaaaatggg gatttaccgg
    37021 aatgttcatc tccgattgct gggcgatccg cgattttcac caaggacacc atgtaaccac
    37081 ctcggtggaa gaatccgccg cactcgctct caacgccggg tgcgaccttg cctgcggtaa
    37141 tgaatatgaa gcattgacga ccgcttttga gaaaggtctt gtcactgtcg gtacaatcaa
    37201 gcaagcggcg actcgggtga tgaccacccg ctatgcattg ggaatgccgg gtgattcaac
    37261 agagtatgac catctgggct tggaacactt ggacagcgat aatcatgctc gggtggccct
    37321 cgaggcgtca cgccgctgtc ttgtcctctt aaaaaatgat gcgttcctcc cgcttgctcg
    37381 aaacaagttt aagacaatcg ctgtcattgg cccgaatgcg gataaccgaa ccgctctctg
    37441 gggcaactat cacggaactt cgagccgata tactaccatt ttggagggac ttcgctccta
    37501 caccaagggg tttgcaacaa ttcgctatag cgaaggatcc ggtctcacta aacggaatgt
    37561 agaacgactt gctcaagcgg atgatcgcgt cagtgaggcg gtgtttatgg ccaaaaacag
    37621 cgatctggtt ctactgtgtc tcggactgga tgaacgtgtc gaaggagaga tgcgtgatga
    37681 tggcagtggt ggctgggccg gggacaagag cgatctgcgc ttacccgaag cacaacggat
    37741 tcttctcaat gcggtagctc aagtcgggaa acctaccatt gtgattcttc tctccggtgg
    37801 agcgatcgac cctgagatcg aagcctatcc aaacatccga gccctcatcc aaggatggta
    37861 tcccggtcaa caaggcggac aagcactggc ggagcttttg ttcggggagt acagtccatc
    37921 cggcaaatta ccggtcacct tttataaagc gagtgcacaa cttcccgact ttgaagatta
    37981 ttcgatggct agaaggacct atcggtattg caatcatgat gatgtactgt atccctttgg
    38041 ttttggactt tcctatggtt ctttttcgta ttcgctgtta acagtccagg aaggtgagaa
    38101 cgattcagtc attgtcaaag ggcttgtcac caacacttca gctattcctg ctcgaacaat
    38161 cgctcaactg tatctcaggg tttcctcatc cgatgcgcct ccgcaccctg ttcttgtcgg
    38221 agtgaacagt attcaccttc tccctcatga agaacatgta atcctattca ccgtcgacaa
    38281 aaaacatttt actatggtag atgaacatgg aacccgttat ccagtatacg gaagatatac
    38341 actgtttttt ggctcaagcc agccggacaa aaaaagcaaa gagctgggag ctgaaccata
    38401 tgaccgattg gatcgtattc gctaggagat attcatgtcg tcaaagattc ctatgagacg
    38461 taagaaaact accttcttga ccaacctgag aaatcaaaaa tacctgctgc tcatgctcat
    38521 gcccggggtc atttggtatc tgttgttcaa gtatttacct cttttggggc tctcactcgg
    38581 atttaccgac tatggattcc gagccaaagt ctcatttgtc ggactggata attttaaacg
    38641 tctgttgact tcaacaatct tttggaacgc gtttcgcaac accttgatca tcagtttggc
    38701 aaacatcatt ttctacttcc ccactcccct catcgtcgcc ctgctcatca acgaactcaa
    38761 aagcgtgaag accaagcgat tcatccaatt tcttatttac atcccctact tcttcagctg
    38821 ggtcgtggta ggaagcatct tcgtgaacct gctctctcct tccacaggat tggtgaacag
    38881 cgtgattacg aaattcggag gaaaaccgat atatttcatg gcaagcgtga aacatttccg
    38941 ccctgtcctg atcacatcat acatctggcg ccagatgggc tatggagccg ttatctacgt
    39001 ggcgagtctg accaccgtcc aaccggagct ttacgaagcg gccacgatag acggggcggg
    39061 acattggggt cgactctggc acgtcaccct tcctgcaatt cgttcaacga tcgtcacgat
    39121 gcttttactg aatctgagcc acgtcctgct gatcttcgaa caggtattgg tcatgtataa
    39181 tgcggcggtc tacggagtag cggatgtcct ccagacgtac gtcttccgag aaggtgtcct
    39241 ttccggggac ttggggtatt cgatagcggt cggtatgttc acctcggtca tcagcctgac
    39301 gctggttctt tcaaccaaca agttcagttc tcgttttctc gaagaaccga ttctgtagga
    39361 gccacccatg ataaaaagca atacaatcag agagtcaaca agccgaaagg tcttccgggc
    39421 attcaatatg atctttttga cgttcgtgtg tctcatcttt attatcccaa tctggaacgt
    39481 gctcatcaca tcggtggcaa aagatatcga cgtaatggga tccgattatc ttctggttcc
    39541 ccgatcgttt accctgcaga actactggag agttctgaac agcggctaca tgggcgcatt
    39601 caagaactcg ctcaaggtcg cggtcctcgg caccattttt tcgatgctca ttaccgttcc
    39661 catgggcttc gccttatccc agaaacatct ccttggtcgg tcgatcatca tgaaagcgat
    39721 cgtcttcacg atggtctttg atgtaggaat catgccattt tatatcgtcg tccgatcatt
    39781 gggactcatc aacaagaccg cagcgatcat tattcccgtc ggactctcca ccttcaactt
    39841 aatcatcctc aaaaactata tgggatcaat ccctgtttcc ctcttggaaa gcgccaaact
    39901 cgacgggtgc aatgatgcaa cgatcttgtt gaagattgtt ctccccctct ccgtttcgat
    39961 tctcgccgcc gtgattctct tctattttgt aagctactgg aaccgatact ttgaagtgat
    40021 catgtttatc aacgacagtc gtaaatatac cctccaagtc gtattgcgct cattgatgtt
    40081 cgaatcggac gattcactcg gtggaggaca gtatgtgtac aacaatctga aaatggcagt
    40141 gatggttctg gggatgttgc ccgtcctcat catttatccc tttgtccaaa aacacttcgt
    40201 ctcaggtatc atgcttggcg gagtcaaagg atgacgacac cttcctccat cgcttcattt
    40261 caagcggtgg agggtcaatc ttccaattca ggtaggaaat cctcccgcat cggactgaag
    40321 aaatcgagaa gtattccctc ttccagacag accgctccgt gaaccacgtt gggttccttg
    40381 tagaggctgt ctccggctct gacaatcttc tttactccgt tgatatcaaa ttcgaacgcc
    40441 ccgctgacca cgtagctgat ctgctcatga ggatgactgt gcaaggatcc gaccgctcct
    40501 ttttcaaagt gaagctcaca gagcatcgtc cctttgctga aagcaagaat cttccgggtc
    40561 atccctttgc cgaccgcgtt ggccttgcgc tcgctattca gaacaaaatt ctcatatttc
    40621 actacttttc ctcctctgac cacatcttat acgaattgtg tctcatcatc agcatgttca
    40681 tagtacaaga acggtttccc ttcaagcgta agcgtgcttt gggcatagtg gggttcaaga
    40741 gccttcattc gattttgaaa tgcttcgaat gaaccttctt ctttgatgct gccaaccaca
    40801 cacacatacg catgatcgct tgaatacgcc cgcagctcgc aatcagccaa ttcatcattg
    40861 tgggcaacca attcacggga acaccagagt gccagcagcc cttggccttt tcgagcaaat
    40921 acccacctcc cctcccgctc gacgctgtca aacttggcct tcgggaaaaa cacgtgcgta
    40981 aagcggatcg ggtgtgcatc actgatccgg tgaatcgttg ccaacatggc tccttcctgg
    41041 cgaagagccg gaagaatgcc gtttccgaac caatagcccg gtcgcatcga agaagagtcg
    41101 tgggttccgc ccggatggtt cacgaagacg atcgtttcgt ttgaaagagc cgccgaccac
    41161 atgtgctgct gatacccgta cacccccggc tcaaagaacg tcgttccatg gaatcgttcg
    41221 ttgaacgact tcgtatagag gtggatcggt tttccctcgt aatcggtatc accgagggtg
    41281 atgttcttcc agcgttcgac cccgtcggtt ctcggcgatt gaacggatgt aagacaataa
    41341 tcccgctcct tcttcaacat gatgagggcg ttgccgctgg aatacttcac ttccgccggc
    41401 ttctgcatga catcgaccaa tccgttgggg atgggatacg aggatgtcgc ataataactg
    41461 agccacccct caccgaacgt cgtaggcacc ttgggattga caaggttcat caaggcttgg
    41521 gccccttgtt tggagggagt gatgacactg cggtacaccc ggcccatcgg ggcaatgatg
    41581 gaaccgtcaa aggtgtgcaa gctcaaatca tgaagcaggc gatcgatgac gccttgggct
    41641 cgctttgaga tactctcttc cgcatagtca taaatattca acagggcggc gaacgtaaca
    41701 ttcatataga cggtggaaag gaactcttcg aaccccagct cttcgagatc atcaaaccat
    41761 tcattggtga gcacccgtcc aaaatccgcc agctcgcttc cggtcatccg ggctgtcggg
    41821 aaaaccgtcg tcgggtacat ctctcccgcg agcaatgcgg acgagtagaa gaggatactg
    41881 tggttttcac tccagaagca catcccgtcc gatccgtcca tcgacatcca atatcgataa
    41941 ttgatcaaca cctcatcgat ccgcgctttc agctcatcat cgatcgggta gcttttcata
    42001 tagcggatga ccccggagac aaggaaatcg gaacaatcat accggtcttc aatttgttga
    42061 agggtcgtga agaagtattc acgatcccgc ggatgttcga ttcccagtgc tttccgggca
    42121 aggatgttct gaatataaaa gccgaacttg tctccccgtg aaagcccgtc agcgtcagcg
    42181 atgaccctca gcattcgccg gtagttctct ttccggtcat gaacgttggt gtactccggc
    42241 ttctgccgtt caatgatttc aaaccgccgt gacagttccc ctcccccgac cggcacccgc
    42301 aaaaagaacg ctgcttcagg tccggcaacc ttcaacggcc ggtcggtgga aaccgtcgtt
    42361 cgacggtaac gggtcggaac ccgggcgaaa tcagggctgg cctcatcatg ggcaagaacg
    42421 atgagccctc tatcttccgg gaagaagagc gtttctcctt cgagtcggac attgtcaaga
    42481 aatgaggcgg ctgcaaggtg ttcatcgtat tcaccggtga gaaccgtcga gagctcagcc
    42541 ggaatctcaa ttccgaaaag catgcgggta tcgcggactc cgagcgtttg cagacggata
    42601 tacagctcat tccttccctc acccaactcc acggtaaaag cgcgcttcat gatgggcttg
    42661 tacaccggct cctcgatcga acacaccttc cgaccgttgc accagacggt cactgccgca
    42721 tacgtccaca gggtaagaga ataagatcct cttttgggtg gttgaagata cgttttcgca
    42781 tcaaagctca catacgtgag cgtcgagtag aacgtcgaga agtcgaccga ccagttcccg
    42841 tgggagtaat agtacgacca tttttgttct tcatcaggat aggcaagatc cttgtgtgca
    42901 atcaaagaac gcagatgttt ttcgtaacgc atcaagctcg catgggttcg caccggcggg
    42961 atgtatgctt tttcaacaag gggcgaggta agatagtgag atataaaccg catgtattat
    43021 ccttttactg ctcccgcagt cattccttgc atgaaatgtc tgctgaaaat gaggtagatg
    43081 acgatcatcg gcagaatcgc cattgtggcg cctgcaaaca cgatgttcca tgctgcagca
    43141 ccgtcaccga cgtttcgcag catgttcact ccgaccgtca gcggtctcat tgaatcgttg
    43201 gacatcgtaa agaccaaggg gaggatgtat tcgttccaac cccctcggaa tgaaagcaac
    43261 gcaatcgtcg ccgtaatcgg tttgagcaaa ggaaacagaa ttcgatagaa tgtctgaaag
    43321 aatgtgcagc catctatctt cgccgcttca tcaagctctt ttggaatggt gttgacaaat
    43381 cctctgttta aaaagatgaa tgtcgcctgg cccgtaccga ccagaatgag caccacgctc
    43441 accaacgagg tgttcatctt cagtttcacc gccagctcaa agagcggcct caaagttacc
    43501 gatccgacgt ttacgaacat gaacgccacg aacgtcgagt acaaaagctc tttgtacttg
    43561 aagtcttttc gcgagaacac atagccggac atgctgctga tgatgacagt aagaatcatc
    43621 gttcctatac cgagaaaaaa cgagtttttc gtatagaccg aaaaattcgc attctgccac
    43681 gcttgcgtgt agttctccaa gacaaagcgt ttgggcaaca gattcgctcc tccaagcagg
    43741 agctctttat tctctttaaa ggaacccaaa ctgatataaa tcacgggata cagcaggaga
    43801 agggcacaga aaccgatgaa gatggtgagt gcccaaatga ccgccttttg cttgttgctc
    43861 cgaatataat tcgcactact catatcgttt ctcctaaaac acatcatcca gcttgcgagc
    43921 cagatagaga tacccggcgg tgatgactcc gatgatcaga gcggcaagaa cactgagtac
    43981 cgtagcatac ccgatttgcg gcgaggaaaa agcggcgaaa atgaggttgt agatatagga
    44041 gaacatgaca tgcgagcgtc cgtccggacc tccgttggtc aaaacaagaa tcgactgata
    44101 atctttgaat gcggtggtaa tcgccagaag gagaattact ttcagaatgg ggctcaacat
    44161 cggcagggtg attttaaaga aggtctgagt tccattcgca ccgtcaatca tgcaactctc
    44221 atagatttcc tcgcttattg aagagaggcc gctgatgaaa taaatcatgt agtttcccaa
    44281 tccgccccac acggccacga cgatgatgct cttcatcacc cacttcgggt cgccaagcca
    44341 gtcgattggt ctggtaataa gattcatcgc ttccaaaaca gtgttcagag ggcctcggaa
    44401 gactgcgaag atgaatgtaa aaatcaagct gtatacggaa gcgctgatca ccgtgggcag
    44461 gaaaaagatg atgcgaaatg tattgcttcc cggcagattt cggctgatga acacagccag
    44521 gccaagggaa atgggaatga tcaggataag cttgccgatg gcatattcaa aggtgtgtaa
    44581 aacacttttc caaaaaaccg catccgtaaa cgcgcgcttg atattgtatg ttccggtaaa
    44641 gtaggcgaca aacccattat agtcgtaaag aacgtaacgg aagagccaaa agaatggata
    44701 gactgatgta atcaccaaaa ggagaaccgc cggactgagc atcagtgttg ccgatatgtt
    44761 ctcatttctt cgtagttttc ggacatcgtg tgtcataaat gataccttta tggtgtaatg
    44821 aacgagtccc gaccacactc agatggacgt ggtcgggacg tttacacaac gttgttattt
    44881 atccaggaac tggaccttct ctttgtttgg attgagcggg tcgaagttcg ggatgacaat
    44941 ccgcttcacc ttgccgatct tgaggtctcg ttcaagcgct tcattgtagc gcttgttcaa
    45001 gtcggcgata agtcggtcaa catccgatcc gttgacgatc gcattccaaa tggagactgc
    45061 gtagttgtct ccggcaacgg taaccgcagg aacggaggga tacagacctt cgtagggaac
    45121 cggagcaaag tcgccaaggc ggccggcacc gctcatgtca acgacctttt gcatataggt
    45181 gctcaaagga agggcgtaac ccttttcaat ataccccttc acgacttcct cgctgttgag
    45241 gtatttgacc acttcccaag ccgcatcctt gttcttcgaa gaagagaaca tgaacagacc
    45301 tttctgggga cgagagtcga cggtaccctt gacggtgccg tcaagcgaag gaagctcggc
    45361 aacggcccat tcgaaatctt caatcgggaa ttggtcggtg aatacaccgg cctcctgact
    45421 ggcatttccc cagatgccga aggttccgtt ggcgaactgg gcgcgcatcg catctacgcc
    45481 ctgagtgttg gaaccgggga acatcgaacc gttggagaag aacttctgag caagcagaat
    45541 accctcgcgg tatccgtcaa aattgaagac tccctttttg aaatcgtagt ggtacacacc
    45601 gcttttgttg atgatgcctt caagccagcg ctcaaattgg gcggatgcac aggttgcgat
    45661 tccgtagaat tggccgtttc cgactttcgt cactttatcc gccgcctcga caagaccggc
    45721 cagcgtcgtc gggaacttgg tgattcccgc ctttttaaag agatcgacgt tgtaaatcat
    45781 tctggtcccg ctgcgtctta cattgaaaat ggcgtacgga acaccgttga tggcgttgac
    45841 accttcaaca atattctttc gtgcatcggt gactttggcg aattcgggat cgtccagcag
    45901 ggagtccgga tagggcgtca acatattggc atcgacaaac gttttaagat cgaatcccgt
    45961 tcctcgagca ttcatgtaga acagatccgg agcgttgccg ctggtaaaac ccatcaccag
    46021 catgttggga tagttctcaa caaccgtggt gtgtttgatt tcaatcgtgt cgctcttggc
    46081 gttgaactct gaaaccttgg cgaggagata ttcgaggtcg tgacggtcat tggaccagat
    46141 ctccacgacg gttttacctt tgggtgcttg cgattcaccc gctccgccgg caaacatcat
    46201 cgatgttgca aacagaaaga tgagcagaat agatagtact ttcttcattg tagtttctcc
    46261 tttatattga gggtatcatg ctgccactga aagaaaatga ccgacatttc gaagaatgta
    46321 accttttttg cgatgtagtt gacaagagtt agaaatgtgt taaaccattt atagtaggtg
    46381 acttatggat agtgaactca ggattctcgt ccttgatgac gaaccgccga ttcgaaaggg
    46441 tatttgcacc ctcatcaccc acaagctgcc ttcggtgcac attgtgggcc aaggctccaa
    46501 cggattggag ggcttggaat tatatcgctc cacccaaccc aatctcatta tcaccgacat
    46561 tgtgatgccg gagatgaccg gccttgaatt tctgagggag gtccgcaagg ccgacctaaa
    46621 taccccgtgc atcgtaatca gcggatatga tgaattttcg tatgcccgtg aggcgatccg
    46681 gctcggagcg accgattatc ttttaaaacc gatatcttca acggaattga tccgggcgat
    46741 catcaaagcg accgacagtg ctcatgaggc gaaacatacg gactacgaag aattgcggtc
    46801 aagcgcgtcg acattgtttc ttcaccgcct gcttaacgga gaactggtgg agatcgagaa
    46861 ggtacttcaa gcatgcaaaa cacttgagat atcgctgcag gagggggcgg ttacggtcat
    46921 caccctcgcc tcacccaacg cggaggaaat tacccgggcg ttgcagtggg ctcaaacacg
    46981 ggccgttacc gtcacgcaac gcggtccgca tggggtcttg ttcatccaag gctcggcgac
    47041 tcgggccgat catttggcgg cggatattct ctcactcaac ctccccgctc tgcgagtcgg
    47101 aatcggccaa cctgttacaa ccttgtcttc agtacacatc tcctttgaaa gcgctcttca
    47161 agcgctcacc taccaagcgt actatcctca cgaacgacta ttcactcagg agatgatcga
    47221 tcgaagcacc ccgtatcttg actcgaccga cattgacacg cttcgtctta aggaaattct
    47281 tctcctcggt gacgaggatg agcttgtcac ctggttcaat acctttttct cctctctctt
    47341 ttatataaag acgccgccgt tgagctatct caaaggaatg tgcatctatt tgctcacgac
    47401 ggtccacaaa catcttgggg aagatctggg catcacgaag tcgtacttcg atcgggttgc
    47461 tccattcaat gcaagcaccg tagtctccat cgatgatctg tacaacgagg tgctgaacca
    47521 tctcgtcgta ttgaataaca aggttatcgt tcaggcgatc gccgacagcg atatcgtcat
    47581 ccatcaggca aagcaatatg ttgaaaatca tcttaccgaa ataatctcat cgactgaaat
    47641 cgccgaaaaa ctcaagatga gcccgaccta cttttccacc tatttcaaaa ccaagaccgg
    47701 gcaaacattc cgttcgtacg taaactcccg tcgaaatgaa tatgcaaaaa gcctgcttgc
    47761 aaatatcacg atgagcattg aagagatttc cgaacaattg ggctacaccg actatcgttc
    47821 tttccatcga atcttcaaac ggatgaattc gatcactccc agtgaatatc gaaaacgcat
    47881 caccaagaga taagcaatga aaaaaatacg gtattggtat tcaagtctcc cgctttcctc
    47941 taaaataacg cttttaatgg ccctcctgat caccctgctt gccggctctt tctccctctt
    48001 ttcaatctct tttttcaatc aggcatattc ccatgagacc gagcagatcg cccgccagag
    48061 attggaactt tttgccgaaa atcttgatcg ggacctgttg gcgatcaaca accagataat
    48121 cgaaacatca acgatgccgc tctttgtgcg gacgattcaa cgggcactgg aagtaccgag
    48181 caaagacctc gagaccttgg tcaattttca gcaattcttc aaacgaatcg ccgattcaag
    48241 tccactcatc cactcggttc atgtgataac aaaagatgac atcctctatt cgccgtacga
    48301 gcaaacatca agaactctcg gaaatgacct tagctacccc tctctaagcc acttgcaaaa
    48361 ggtcacgctc ctgcccggcg gtcaaaaccc tttcgctcca agtcggaaga gcgtccccat
    48421 tgtcgtcgcc ttgaaccttc tcggcacctc atcatatctg gagcttacaa gcaaccttgc
    48481 tgctgacgtt tttctaatca tcctccttga tgaacaagcc cttctggaga agattaactc
    48541 acccggatcg gcatttttct cttacacaaa cacgctttta ttccaaggag tgccactcct
    48601 gcccgaggct cccgagtccg attccgcaat gatccgcttt gccaccccca cacagataaa
    48661 cggtcttgaa ttaatgatga gtattgccaa aagatcatat aaaccattgg tcgcgttcat
    48721 gatcatcata accatcatta ccgctttgtt ggtcaccggc ggtggcagcg tgctcatctc
    48781 aaggatttcc cggcatatca caagtccttt ttccgctatg acaaaaatgg tgaaagagat
    48841 ggaaacgggt tcatacgagt ttgacatcaa acctcgttat gccgatgaaa ccggaacgct
    48901 tatcgagggc atgaactcga tgtaccatac gatcattcag caaatgcggc aaataaaaga
    48961 agaggaacag caaaagtatc gatatctttc tcaaatgctc accgagcaga taaatccgca
    49021 tttcatctat aacactcttg aaataatcaa catggaagtc atcaacggac gccaggaaaa
    49081 tgccagcgac atgattgcca cgtttgccgc ttttttacgc cacaccttga accaaggcga
    49141 ggacaccacg acactggctc aagagctgga gcactgccgc aaatatctgt caatcatgaa
    49201 tacccgcctg ggcttgcaga ttctcatctt tgacgatatc gcaccacact tggaactctt
    49261 tgagattcca aaatcaatcc ttcttccact tgtcgaaaat gcgattcggc acggctttga
    49321 aacgtcttcc atgcaagctc aaactgttga acacccgtcg atcacaatca ccgcaaaaca
    49381 aaaagaagac cgatattcga tcggagtcat cgataacgga acaaaaggga ttgatatcga
    49441 gcaagcgtac aaagccctga atgaaaagag cgctcagtcg ggacacatcg gcctcaataa
    49501 tgttctaaat cgcctcaaac tctatttcga cgatgttgag gctcatgtct catccattcc
    49561 gggctatcga aacgcgatca ccttcacgtt ctcaatcgat ccagatcggg gtgctccaag
    49621 cggtatcgcc gctttggctg aatgagcgaa catagaggct gctgcctgaa tcaagatcca
    49681 aagactcatg aatctcaagc atgcactcac tgagatcgat ctcttcactc aggcggagag
    49741 ccggtgaaac aaagccatgc gtgtagagga cgctcggccc atgttgaagt tcatcgcttt
    49801 gaagcaaaag gtcatgcatg aacgtttcac cggcgacttc ggcttctaca tgcaggatga
    49861 tcgagtacgg cccatccgtc tccagaccga agccaaagcc ttgagtattg tcggtagtcg
    49921 ctgagatatt tcccgaggta ttgcagctca tcgaaatcga aacgccatca aagcgcagac
    49981 tcggtaccgc caacgcctca tccgtcggaa cactcaaggg atcgacaaca tattcaccct
    50041 tgaagcgcgg aacgggatcg acgatcgcgg ctccttcggc tcgaagtgtt accttccagt
    50101 gggcggatac gtctcgctgc ccccaaccga gtgaaagatt gaagcgcttt gacgaactcc
    50161 tggccggatt cggacggaac gttttcacta ccagattgtc aacaagcaat tccgcgtagg
    50221 caagcgcgga gggtgcaaga atataggaat caaacctcat cggcttcccc gttgccttga
    50281 cgacactgcc gatcggccgg tcttccagaa atgatgcaat ccggatgggg tctccggtta
    50341 aggcgatcgt tcgtttcgcc aacagaccat cccagattcc atcttgagac ttctctttcg
    50401 cccagacacc cgccctcccc gagccatatg ccccgggcga cgcattgtga tggtccgttg
    50461 aaccgacgac tccaaagcgg aagcctcggg ccagaccgcc ctgcatcgtg ttttccccgc
    50521 agacgggccc catcgtatgc aaatatgcgg gggttgcata ccgggattcc gatgcgccgt
    50581 gcatcgacat gatttcaacg attggactgg cggtttcatt gaatcgatcc caattgattc
    50641 cgcggtaccc cttcttatat ccgatatggt gaggcatgca gatgattccg ctttttctcg
    50701 ggtcattcgt cgccaagtac tcatcaagac gggtatctat cccttcgggg tcgggcggaa
    50761 agccttcata caggtctttt cggataatcg tgtagtctcc gagcttgaaa ctgtggaact
    50821 cgtacgaaac gaagggaaca attccgccgg atgcgctcaa gcgcatcgcc tcaaggtatt
    50881 cttcccatac accccgaagc ttggcgaaac cttccttgtg gtaatcaaca accctttggg
    50941 gaatcggttg ggatccatct tccatatccg gccaagcgaa atgtccggta acggtaaaga
    51001 aatccaattg gctttgggca aaggcgatcg cttttgacag gctgccatga ccgtagctga
    51061 tcgcacagtg gttgtgcaga tcaccgagat acatccggtg ttcttcaagc cactgctttt
    51121 gtgcggcgga aaccgatgcg gcaaagacct gttgtattgt tttcatgagt ttgttcccta
    51181 tttattgtat ttccatgaca gatgaatccg ttgtttcttg ttcttgctcg gcacatcaat
    51241 tcgaagggct tggaagcccg gacaaaggtt gaaaattcgt tcctcaccat acggtaactc
    51301 aaatcccggt ttgctctcaa gcgtcactgt cg
//
LOCUS       DAOSLI010000007        50944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_790_length_50944_cov_27.668651, whole genome shotgun sequence.
ACCESSION   DAOSLI010000007 DAOSLI010000000
VERSION     DAOSLI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 50944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50944
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_50944_cov_27.668651"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLF67_02100"
     CDS             <1..536
                     /locus_tag="PLF67_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE88838.1"
                     /translation="GRKEYLQSLAYTDALTGLGNRRAFDEELIRLAKSDSKEKQYGIV
                     VFDVNSLKQINDDYGHAEGDLVLREIGELLTSLYGTFATIYRIGGDEFALICEQCGQE
                     RMRDFERLFTEAIAKKPFTLAFGSALSSLDRSDSSDSLYKLADRRMYQQKKTMQYKTY
                     HKSPQNPASIDTSEGTR"
     gene            561..1928
                     /gene="argH"
                     /locus_tag="PLF67_02105"
     CDS             561..1928
                     /gene="argH"
                     /locus_tag="PLF67_02105"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOE88839.1"
                     /translation="MSKLWQKDYDLDSLMETFTVGDDYILDQHLVIADALGSIAHARG
                     LEKPGILSEEETAELTQGLIDIIGLRLQDDFPIHKSDEDCHTAIEGFLVKRVGEAGKK
                     IHTGRSRNDQVQTALRLWMREFAYKLAHKGAALATMLFDFAEKHRDVPMPGRTHMQLA
                     MPSSVGLWASGYAEELADESKYLLSIADLLDQSPLGSAASYGVPLDLDRNYTSALMGF
                     SRVQNNVLYANNSRGKFEAILLDGCDYLGLTLSKLAQDLMIFTLPEIGYFSLPNELTT
                     GSSIMPQKKNPDGLELARSRSSVISSCAFRVKSIIRSLPSGYNRDFQDTKGALFEGAK
                     ATWLLLGIFEVMIEGLIVHEEALVKGCKPELYATDLVLKRSLAGESFRDLYREVGLHL
                     EEVLPGDPYEALRERTSVGTAGNLNIDRSRAGKVIRESETRLKAIQASYEALSGLKQL
                     EVVSF"
     gene            complement(1925..3664)
                     /locus_tag="PLF67_02110"
     CDS             complement(1925..3664)
                     /locus_tag="PLF67_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOE88840.1"
                     /translation="MKAVMIFFQIIGSLGLFLYGMKLLSEGLQKSAGNRMKAILNMMT
                     KNRFISIMTGVIITVIIQSSSATTVMVVSFVNAGLMELVQAIGVILGANIGTTVTGWI
                     VALLGFKMNISLLALTSIAFALPLLFSKRSRNRDKSDVLLGFGLLFLGLNSMSNSIPS
                     LSDNPQALAFLATFNANTFWTNFLCLLVGLVVTVIIQSSSATTAIILTMGYNGWVGVE
                     EGAAMILGAMIGTTITAYLASIGTSTTAKQAAWAHILFNVLGSSLALIFFRPLLALVN
                     WIVPGDVFILTGDALAQALPVFLAMFATVAAVSKTILMFPFVTLYARLIERLFPQKAE
                     YEEGTYHFRYIGAPFIDSPEIYMLAIRDEIKKMANLACTMLARYRSMFDSPNNAIDDL
                     VDLMRRDEEYADQMQEQLSNFIVHLLQDSQTTTNASSLNSLIRVMDELESVTDSCYAL
                     ARLSQRRYKMGWNYEDEILKEMETFHLIVQEFLNYVRDRMDRTLTKAEMQKANEYEDQ
                     INALRADLGNKVQDRLSEGVGDVKAELLILELIRHLEHIGDYCTNIAEAYHQAVKHTP
                     VLQKRSQRIVETA"
     gene            3767..5062
                     /gene="prs"
                     /locus_tag="PLF67_02115"
     CDS             3767..5062
                     /gene="prs"
                     /locus_tag="PLF67_02115"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOE88841.1"
                     /translation="MSIIKPQKLGIISGPGSEYFTGKVVKHLRRLYLERYYKLSSALA
                     KRHGISEEEILKTVTLTDDLSNKRIPRSKAPTTFQCPDFTIKTKYVRFANGEVKAEIL
                     DPVRGLRVFIIHDLSNNEPVRVSGSDEPLRLTINDHLVFLLTTINALTLAGADSVTLV
                     IPTYPYSRQHKKSTREALTASMFGRIFEMLKVERIITLDIHSREIENSFGHLHLENLH
                     ASYQTLIALRKLVDFSDPNLVVVAPDTGAISRNKFYAQALHRPFAMLYKERDYSVVSK
                     DARNSNIKSINLLGDVKGKTVLIADDMLATGGTMIIAMRELTNLGAEKIICMVSLPFF
                     NGTAVEDFDQAYREGIFYRIIGTNAVTHGRDLLDKEWYIQADITELFARVISRLHHGR
                     SISPLLDNRRFIQILIDNTQANPKAPLPDAAATDPDQED"
     gene            5131..6327
                     /locus_tag="PLF67_02120"
     CDS             5131..6327
                     /locus_tag="PLF67_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE88842.1"
                     /translation="MKMSNRISSFQASPIRRLSPYARSAVKRGITVYHLNIGQPDIKT
                     PQVVLEAIRSYNEPIIAYGSSEGYERLRNALPAYYATYGVDIKPEDILITTGGSEALL
                     FTLMTLCDPGDQVIIPEPFYTNVSSFAHSAMVELVPVTSRLEDAFALPPMKDLEKLIN
                     PKTKAILLCSPNNPTGHVYSKEDLERTLQLCKKHDIFLVVDEVYREFCYDGERFTSIL
                     TFEEYADRVVCVDSFSKRYSMCGARVGMLVSRNKQFLTDTLKLSQARLCPPDIEQVAA
                     AAALTTDPSYLVEVKKEYESRRNFLVEALEKVEGVKTTKPKGAFYIIAELPVEDAEDF
                     ATFLLRDFDLNGETVMLAPAEDFYATKGIGRRQVRIAYVLNKEDLGKAVACLAAGLKA
                     YAERGA"
     gene            complement(6324..6743)
                     /locus_tag="PLF67_02125"
     CDS             complement(6324..6743)
                     /locus_tag="PLF67_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOE88843.1"
                     /translation="MVTYRKELWFHLNKRRGLVNITEAIGEAVTESGIKEGLVLANAM
                     NITASVFINSVEEGLHEDFEKWLEELAPELPYERYRHNTEKEKNADAHLKRTIMGRET
                     VVAITGGKLDLGPWEQIFYYEFDGLRDKRVLIKIIGS"
     gene            6829..9567
                     /locus_tag="PLF67_02130"
     CDS             6829..9567
                     /locus_tag="PLF67_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88844.1"
                     /translation="MKTNESWDLSSVGDISAQKIRRNLRELAVFSANSIRSLKETAFR
                     YEPIALMEQALEHYHRTVIREWKGDGEAIRISKALISYLANLLQSFSKPLSKNRQVKQ
                     KDWKDIVRLFDDIVRKSIRIVDNTALALKANGTLTDPILLERFQKEASAWVLPPLVDE
                     ELLKRHHRSLLYQLQPYNALISEVFPSHLDGVLQALSTLIETDEHDLLNHTALSEADA
                     KVLARPIASEEFRVDNHRLTQFQQSSLKPFILINGSYYCFDPERVLIEGYGIIKGAVT
                     TSSGERKEAWERIEKEKRSLLSVTFFTAMLSSMHYTRKVEYKDGFLDALFEDGKKELV
                     IQIPAAHTVFPEDPIQESAAYARALIEEQKAIDLAKTHPAPSVIIDLRAIKSYPVTLI
                     DNALTLSFLQVAALATTWEGANEIKGELGLLPLGDLTPPPHDKEPEEQLEAEQPKAGQ
                     TDDEHDPMKEFDEHLEDELEWEEEEDEDSFDDDEILEEELSAIDEEDEYEDDLEEFEG
                     DEQDEYTAILEDDEYEGEAPEPIEEGKEEPEFTLEAEVDSSWQRFDQGFTLGIKDPRI
                     IEGEYLDYADEEGEEEEDDWAYSGSSYFTSANPLEDYLGEDEYETADELFSQSEQPVL
                     FDVEDERMFDPFYRTDLERQAVEEDYESEAIGLFEYESEGSGYDEEEVLVPSSFVLAD
                     EEMEEGKEDLLRYHTQYVEDDDGDEFGFLDQIQRSAEQQKRPSSRFTLINAVEHAKHQ
                     PPPQEEEDKKAPPAPPPAAKQASVEPFELDLPPNLSEIFTLLAKKNQWSFNTYLKESM
                     AHEIDSLERMLQQIRDTTLEPGQDRMFTIVPMELTIIVLAAKGDPLSTWTRKVNVAAL
                     MHANKKRSWRALSIGYDRKRNLVFADDCPIRREDFTATDWKFVINAANRLRDKKRV"
     gene            9575..10351
                     /locus_tag="PLF67_02135"
     CDS             9575..10351
                     /locus_tag="PLF67_02135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOE88845.1"
                     /translation="MKTHLDVALQAARRAGSYALGAERNGAISVRMKEANDYVTDVDI
                     QIEQLIKDTIRLSFPHDGIFGEEGGESGNLQSNRWVIDPIDGTTNFFRGMPNYTISIA
                     WEEEPFHPLVGVVFNPRHNELFWAAKGQGAFLNGQKITVSSVRELNQAIVVLVPPHRR
                     RDLADSYFETMNTIFKATSDIRSFGSCALELSYIASGRVDGYYERALGYYDMAAGMII
                     LQEAGGIVTPAFEDAPFSDRSCDILASNGHLHRALSSLIA"
     gene            10383..11024
                     /locus_tag="PLF67_02140"
     CDS             10383..11024
                     /locus_tag="PLF67_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOE88846.1"
                     /translation="MKFKLAIFDFDGTIMDTSPGIFSTANATIESLGLEPEKDPSVLA
                     RFVGPPIRESFVAVYNLDESLLDEAVTRYRAIYDRTGRFGAELYEGIDRMLAALKAAG
                     YRLAIATLKSEPVVKQMTDHFGLTPYFDSIRGSDPIHKRSKAAVIHQVLEDLQIKAEE
                     AVLIGDTPHDEEGAGEAGSAFIAVDWGFGFPKGYRPSSTMVAESPLNVLTFLL"
     gene            11069..11464
                     /locus_tag="PLF67_02145"
     CDS             11069..11464
                     /locus_tag="PLF67_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOE88847.1"
                     /translation="MSFQVTKSEVATPNAPKAIGPYSQAVSAGPFVFTSGVLPVDPKT
                     GKLVEGGIVEHTKQVFENLKAILKEAGTSLSNAVKTTVFIASMKDFAAMNEVYASYLG
                     DGVKPSRSTVEIPNLALGALVEIEMIVLR"
     gene            11474..12559
                     /gene="gcvT"
                     /locus_tag="PLF67_02150"
     CDS             11474..12559
                     /gene="gcvT"
                     /locus_tag="PLF67_02150"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOE88848.1"
                     /translation="MNRTPLFEHYGEHAKLVDFGGWEMPLHFEGGILAEHEAVRKAAG
                     LFDVSHMGECVVTGEEAGAYLDWLLTNRVSDMEVGQCRYTMMCYPDGTVVDDILVYRR
                     HSESYMLVLNAANTKKDLDWITKDNPKADRAPFVVDISASTALLALQGPQAAGILSRF
                     IGDIDEVEGFTFRSQCTIGSILCLVSRTGYTGEDGFEIYCDRDDGPKLWSLLIGEGAV
                     PCGLGARDTLRLEAKLPLYGQEISDSITPLEANLGAFVKFEKEDFCGKAALLEQKNKG
                     VPRTLRGIEMIDKSVPRTGYPVFRDGKEIGYVTSGMKSPSMDIFFALVLIDREPALKF
                     GDELEVEIHRKLKKARLIRTPIYQRSH"
     gene            12561..13859
                     /locus_tag="PLF67_02155"
     CDS             12561..13859
                     /locus_tag="PLF67_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88849.1"
                     /translation="MLKIETRVPEGFIALDNSALIYPPTEAAFNANTFRISIDLKEAI
                     EPERLKRAFKDLLKRCPYVNVTMKKGFFWYYLTPNPKDPIIHPEGSYPAGRFLFRENN
                     GYLIRVLHASHRIALECFHALSDGIGVLELLKLLVERYLASGGREVSFQGDLSFDEPA
                     QAWESSDPFQTLYDPRLPGYPSYPRAYHYKGSGSYDERVKVISARMKSSDLKGAAKLH
                     DLTIGQYLGAVFIHSLQELQLKEGRKMRRRKPIRISVPMNLRRLFDLKTMRNFTLFAV
                     VGIEPNRGYYSFEEIARHVRLQTALAQDKKALLSQIRRNVAGEHALALRFVPTMLKNP
                     FFKLLSDHLGDQQYSGVISNLGHVALPGLLEELVERMDFHLSPGLLNKVVLSVIGYKD
                     EVVLNFSSFFTTDTELERIYCERLIHDHVPVHIASNRGGL"
     gene            13859..14746
                     /locus_tag="PLF67_02160"
     CDS             13859..14746
                     /locus_tag="PLF67_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88850.1"
                     /translation="MAYCVRCGVQLAGGSKRCPLCDTPVLLPDGFIEEIERPLFSKPL
                     ERAQKGGLSKARKGILELMIALGVVAFISVGLALGLSGHRDIVLIPLVAIAVSLVSLS
                     YVLMGRQTYVAQSTVHLTLSAVLLIVIDGTLGRISWSLIATFSIALFWVLWVFPFMKH
                     PELDLPRKLATSMAAVLFYLGGLNRVLDGKFTWFVPIALPLWSFTVTATVVLLTSFAA
                     RRGRTVTITELVLSTLFIVFLALTGLDLLQNHYRNGAWALRWSAPLLIGAAVLLVVLL
                     AYVLSLRVRRYFTSSRTPR"
     gene            complement(14739..15203)
                     /gene="ybaK"
                     /locus_tag="PLF67_02165"
     CDS             complement(14739..15203)
                     /gene="ybaK"
                     /locus_tag="PLF67_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOE88851.1"
                     /translation="MKTNAMRILEAGKIPFELVSYRFDEEALDAIHAAQTVGLDPKTV
                     FKTIVLVNERKQYWVFCLPADRSINMKKARSLTGSKALELVKLTEIQNVTGYLRGGCS
                     PIGMKRPFPTFIEEAAFSLPFIYVSAGKRGMQVRLDPSDLLRSVDGKRADFT"
     gene            15379..16968
                     /locus_tag="PLF67_02170"
     CDS             15379..16968
                     /locus_tag="PLF67_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOE88852.1"
                     /translation="MRNAKRCALVLLIIIAIVHGALIAQDNGLVIRSPLYGSSTKDLV
                     GTTIQIQSYAEQLGLAATSTIPTGDERLLNAISEPSYPVTPGDTYRVIYLDGLKTVTL
                     DLQVNEAYQVVLPGLGRVNGQGLTFQQMSQKITDLIEAYHSYSNPQVIFTGVGSFYVS
                     VIGEVTGSRTVQAWGLTRLSQVVGAATPYTSTRTITITHADGSRNTYDLYLALRKGVL
                     TEDPLLKSGDVVSLGRAERLVHIDGHVYRTGSYQLQEGENLSSLITDYAGGVLSGGDI
                     QRIRIQRFDEELNDWTILYADLLKDQQFEVRHLDKVYVDLLGPMPQSILIEGAISSTE
                     AANTLSSSSLTGYVSGRIFYQLYPGETIKHVFETLSGRLLSISDLERAYILRDGQRIN
                     LDLQRILYGQDPNSTRTLENGDAIMIPFSQRFVSVTGGVVRSGMYAYAPNKSSSYYIA
                     LAGGYSDDASFPLSVKVQGEDGRKIAKTEEEVPPSSTIIVKKNTFTKDIAPTVAIVGL
                     VAAILGIVSTTLSIIKDVRSL"
     gene            16978..19545
                     /locus_tag="PLF67_02175"
     CDS             16978..19545
                     /locus_tag="PLF67_02175"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOE88853.1"
                     /translation="MNQAYDDQYTVHQQFDEEGMTIRELLSIFRKRFFWFLLGLVLVS
                     GAAVGYLFWATPLYESQVSVLVNPIQNSSSIDSLLSVTSSGAKIATEVELITSRSNIN
                     EALARLDLTRYENDEGESYAMNIAFPNSVKEKISVTTVKDTNIVRISVTDANRFFARD
                     FANVLAESYDSMLTSIARNSKTAQRTFIESQIPINDQALREANDRLGDFKERSEIIQL
                     SDKSSLLVNQISYYDLRLEPLKLQLEEAYIGFRSHEPFIAASGTTSDQLLTTIRSDEE
                     ISKKLSELESWLLELAMYESLPTNLGQGALSEGTLARIFTINNAIDTTKKSLLNRVRK
                     LIECCYIDSLAQNIAQILTTGVSIDILQGRAEVFEEELSRLPLLERTLSELLRDVQIS
                     EAIGLKLREMLEEIKLLEASVSGNVKVIDTANLPLKPVSPNSLLILAVALLLGSAIGL
                     LLALLVESLDTSIQNEEQVQRIVGRNAPMLGWVPIMKTYQKDVYPMFMVRTHPNSFEA
                     ERLKLIANMIYSMDGKKVFSITSSSMAEGKSMIVANISLALAQLGNKVLVVDGDLRLP
                     SMERFFRLEHQSVGLVDAVTSGVSLEDCIVQPLEDVSTLHLLPAGSSPLVPAAIFSNP
                     RYISWTKMLSNIYDYIIIDAPPLDAASELLAIAKHVDGLIITVRAGVTSRIALRDLLE
                     SLINANVQLAGFVFNGVMPSKHSRYGTYGYSVSYKMGKTERKFGRKYRYLPRRRTASW
                     YRKRYKQDVKIRGKLPALFTPVRAFGPHAPYMNLKEMEEKNNGNGEEKLIFKPVVSGD
                     AGSRSKELAEKRDETGEEKPVFTPAAFKDTPKHPMTEDKVIDFLSAIEGDEAARGKK"
     gene            19712..21298
                     /gene="pyrB"
                     /locus_tag="PLF67_02180"
     CDS             19712..21298
                     /gene="pyrB"
                     /locus_tag="PLF67_02180"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOE88854.1"
                     /translation="MGTFYGRSLCVIDDLSQEERLYLFEHARILKSAFETQDEAVLNR
                     YRIDDPDFGIYEVFLEDSTRTKESFRNAAKFHHAKVSDLNSGSSSFNKGESYADTFYT
                     LSGYSNTIFIVRSKVEGVCTYLEESLTSYARRNGLKRKPAFINAGDGKHEHPTQELLD
                     EFTFLEDNNWSTETLHLALVGDLYHGRTVHSKADGLKIFKNVTVDLIAPPELAMPETY
                     LHKMKDHGFTVRTFDSIDAYLKETAIAPKWYFTRPQLERMGDKVLKKEAELRRAITFR
                     EELIPLLPLNTKFYHPLPRHKAHPTIPTFLDETPLNGWERQSINGMYVRIVLLALIAG
                     KIGGEFVSARPPVVEEEDYIKEVNLEDRVPQEKRISEGVQPIHDGIVIDHILRGYEIG
                     EIRDHMRLISSVLELDRGKGGEWVSSGQQGIHKGIIFRPGSYELDRKHLKRLAAVAPG
                     STLNLIKDGKVQRKFRLSLPPRIYNFEDLGCTNEACISHPSQEEGVMARFYRTQENTF
                     ACAYCGKEHGFKEIWKRSRK"
     gene            21316..22014
                     /locus_tag="PLF67_02185"
     CDS             21316..22014
                     /locus_tag="PLF67_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOE88855.1"
                     /translation="MNNLKKITQAYGPKIARAAFELGAIRLNVTDPFRWVSGYRMPIY
                     NDNRRLLASGKVRALIAEGFEAILNALEYDPDSIAGTATAGIPHATTLADRLGKPLSY
                     VRSSSKGHGLGNRIEGLAKGGTYENTHVLLIEDLISTGLSSTSAVEAIVAAKGQCPYT
                     LAIFTYGFAAAEEAFAALDPKAAFHTILTYEVMLSSALKEGYISRSEAESLEAWSSDP
                     FGWGIERGFMREEG"
     gene            22011..22877
                     /gene="pyrF"
                     /locus_tag="PLF67_02190"
     CDS             22011..22877
                     /gene="pyrF"
                     /locus_tag="PLF67_02190"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOE88856.1"
                     /translation="MTYRELLETSAKQVGNVVCMGLDVKPELLPHAEQGIRHRLNTYF
                     QQLFRRMNLSGLLPAAFKPNIGYYAVLDNPREEEFSGSLALADLFDLLENFFPNIPII
                     LDSKRGDIATSSRNYADEAFNRWRANAITVAPYMGSDSVGPFIQDSTFGTYILNRTSN
                     PGGKDLQNLILHSGIPLYEAVAKQIIAYNAGKGHVGAVVGATNPEELKAIAKTYAEQS
                     IPLLIPGVGSQGGSATDVMRTLGEVNYPLPLVRINSSSALTHPWKKGPAPDDWLDQCE
                     GSLRTLIKECSL"
     gene            22874..24586
                     /locus_tag="PLF67_02195"
     CDS             22874..24586
                     /locus_tag="PLF67_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HOE88857.1"
                     /translation="MSDVLQRLMNQEVLLATVSGVGSTKAELIRYFDESVEAIDLITT
                     KSFQNEPNPGNREPIICEVEKGTFGNSVGLRNPGLEVALHELRELRKTHSFRALLNVS
                     LSASTPEDFVTLVKAFDEVADLVELNFSCPHAEPGYGSTIGENLKLAAHYVRFIKANT
                     VECEAPLFVKLTPNVEDIASIAKAVVEAGADGIVAINTVGPLLYIDPASRKPILQNRL
                     GGKGGQSGKAVFPIALKAIKEIREALGTGIPIIGMGGVDSGADAAKLIEAGANVVGIG
                     SALGTVDQRSWPQYLRAIKEEADHLLYSGKKPAVSSASFIKTDRQMEYRKHIVLENEP
                     YGSDTAILRLDGRLPTKAGQFAFLWLPQIGEKPFSVAHNDPLTFVIKRRGPFSDALCS
                     LRKGDEVYIRGLYGQGFDPPETKKALLIGGGTGVAVLPALAEQLSERGTEVEILVGTS
                     EAVETTALLEEKLSSYGSFVCVADEGKPGRVLDLLDGRTFDGETACYLVGPQVFMALA
                     ARAVLSGGVKEEMLYLSQELMTLCGIGMCGECVCGDRLTCRWGTFMEYRYLKEHAPSL
                     IAYD"
     gene            24579..25625
                     /locus_tag="PLF67_02200"
     CDS             24579..25625
                     /locus_tag="PLF67_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HOE88858.1"
                     /translation="MIDPHVHLRDWNLSAKETLAHGLLVAERSGFTAVIDMPNTDPPL
                     TTKARIIKRIEAANRTKRNVSYHLWAGVTGDPAQIDEVVKSCEELFPSVVGLKLFAGH
                     STGNMGLVEEEIQGWVYRHLARRNYTGAVVLHCEKEALLRPELYDNADLSTQSLARPP
                     EAEVASIRDQLSFSKEAGFAGHLHIAHLSTIEGLLLVEEAKREGRRISCGITPHHLLL
                     NEDTAKDRRLYAKMNPPLRDEETREELFEALLEGRIDWVESDHAPHTIADKEAGASGI
                     PGFSGMLSLLKVLYEHNLSDSVLKDLFGGNVQRTLNLPPREIVLPRYETLSALIAETS
                     GEYPYDSFRGLFQS"
     gene            complement(25603..26154)
                     /locus_tag="PLF67_02205"
     CDS             complement(25603..26154)
                     /locus_tag="PLF67_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOE88859.1"
                     /translation="MVTRSEALALLKKYTPDEAHLHHAYCVEATMKSFARHYGYDETY
                     WSLVGLLHDIDYALYSDRHCQVAPKLLSEIDVDEAFIRAVVSHGWGLCSDVEPVHFME
                     KVLYTIDELTGLIYANALMRPERMKGMSVKSVRKKWSSPSFAKGVNREVIAKGAELLG
                     LEINDIIARTIEALAEISSEIGL"
     gene            26255..26857
                     /locus_tag="PLF67_02210"
     CDS             26255..26857
                     /locus_tag="PLF67_02210"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOE88860.1"
                     /translation="MNIPACTGTYIEEVIASLTDLYTDVENATHAFASRYGIACPPGC
                     GSCCEHFIPDITHSEAAYIAAYLLFELKDEEMIERVYRHSGAGCPLYRADTPFHCSVY
                     PARPLICRLYASSAYHDKYGKALFRSCKYEETNTMPKHLSFDERVVTMQDFAYRLRSL
                     DRDRGRLSLLSELLPELLEQLQFIAALVPAQDGPEEPLAG"
     gene            27080..27748
                     /locus_tag="PLF67_02215"
     CDS             27080..27748
                     /locus_tag="PLF67_02215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE88861.1"
                     /translation="MAGKIKAILFDLDGTLIDTIEDIAAAFSAAIAKDGLEPATVEET
                     KLVVGRGLVNALKGLLEIRNHPVSEERFTELFHVLYTYYEEHPVVYSKPFDGMIELVS
                     GLQDTYQLGVISNKDHELTLRIVSELMGDVPWSVIRGMNDEYPRKPDVGAFTPFVKKM
                     DLGLEEMLLIGDSEVDYQSARGANMDVILVSWGFRPREALVALEGACVVDSVEELKEA
                     IYAL"
     gene            27738..29444
                     /locus_tag="PLF67_02220"
     CDS             27738..29444
                     /locus_tag="PLF67_02220"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOE88862.1"
                     /translation="MRYNEKDLRQKATLYLEVETEEAFRTEVETELENGQWAELYERF
                     YTTLSFGTAGIRGLLGGGTNRMNTFMVRKVTQGLADYLKTQSDHPSVVIGYDSRHYSD
                     VFAKEAALVLAANGISVYLFPELRPVPMISYAVRNLKTTAGIAITASHNPSAYNGFKA
                     YWRDGAQVTPPHDFQIADRANAVLPKQIRTITEEKARSNGLLVRVPDKVEESYYNEVL
                     SSIRNMDIIKTSKTLVAYTPLHGSGLKPVTRLFDEVGIDYVTVKEQEEPDGSFPTVKL
                     PNPEDHDAMALVLRLAKKKGADIVLGTDPDADRLGIAIPLSDEKKEYQLLTGNQIAVL
                     LADYLMMTTGHRTKQPVVVKSIVTTDLVKAIVEKRGGVCKDVLTGFKYIANEMTLLEG
                     PKGEEQYFLFGCEESYGYLTINIVRDKDAVSTALVAVEMLSYWKDRGKTLIDRLGEIY
                     REFGYYTERVISVDFEGAAGQEKMVKVMQSFRDLTAGEELVGRKILQIEDLMNPQEGG
                     LPPSDVVIFHLEGGERVIVRPSGTEPKIKYYLFFSTEAVNREAFDAHLESRVKEMREG
                     LL"
     gene            29441..30187
                     /locus_tag="PLF67_02225"
     CDS             29441..30187
                     /locus_tag="PLF67_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88863.1"
                     /translation="MIECYTFVRYNKPVLTLTPFLREGHDLLGEQVVMYAGGMLNAQQ
                     KDQATFSLFSQIDFAVDRWIQDKRYVPRLLFSALAFMVSYLFFSLVVRDPLPMIDELL
                     ISSGWAIVVWITLSRRDTRSILAQENRQRLKMIGGKRSEQIQENLFSIEEYLDVCAKA
                     DTKELAKQLVEGTLPRWTHTIEASERSLLRTLLDRYLAVYEKPTARWIQRLDRSRGKD
                     LATKLYLQGSEGSVDLSLLALRCALKQSEE"
     gene            30203..31738
                     /locus_tag="PLF67_02230"
     CDS             30203..31738
                     /locus_tag="PLF67_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOE88864.1"
                     /translation="MGLPVLCILFCYLSYSLSPLFDYRYLAGGLFLGSLGLFLRWGSW
                     RRYALCSYSLIIFLLYLLFGRAVHLTRFTCGLETKRITALEGVLLEDSLLTKSANQLL
                     RIKVAWCEAADGSGASASGVCSALGSSDEILFASSTIRVVGAFHEESSLFIAEDLQIR
                     DIPSFARWRRSALEGIRRRLKKSVSDEKARSLAFMLLLGQSDSSAFPLKELARRTGLS
                     HLLALSGMHLSVFLGLSTALCSLFLGSYRAKRAGMVLPCLFVLIAGPKPSLVRALGLA
                     LCSLIPFGSHGRRYSYLLALSLQLLWFPYSVGSLAFLFSWVAYTMIYIAPMLPAFPWK
                     TGTLAVLAIAPATLLLEGSWNAGGIFLTPVAGLLINLSMISSLLVLIGGSSFARVLLR
                     CDGLLNDLLLWGASSNLVFGWKGYAIALAFVLTLFFALIYAKRNLLKQETYELDLSLR
                     FTQRDHRPSGGGGAGDEQEVWTKLPHLELEPEQDRRSARCAGGIEGLGGGTGNRCDHR
                     PSS"
     gene            31653..32435
                     /gene="rsmA"
                     /locus_tag="PLF67_02235"
     CDS             31653..32435
                     /gene="rsmA"
                     /locus_tag="PLF67_02235"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOE88865.1"
                     /translation="MVDLLDVQGGSKVWEVGPGIGAITALLLERGVRLTAFELDHGFC
                     RILRTRAFGDEPHFTLIEGDALKTLFTALREKGTPERLCGNLPYNIGSILVARLLEGE
                     SRPNRMVFTVQKEVAERICAGEGSKNWSSFSLLAQADYDVGIGFDISPGSFYPPPKVT
                     SSVVVFQLRSVSRIPQADRHAFYLLVRELFGQRRKTLRNNLLQSKVGARIGREGVDWL
                     LLQSGIEPSRRPETLLWAEILSLSAHLSSIPATRSEGTSQGR"
     gene            complement(32374..33756)
                     /locus_tag="PLF67_02240"
     CDS             complement(32374..33756)
                     /locus_tag="PLF67_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOE88866.1"
                     /translation="MTEGLLDQEKSIGLIIWTLAWPAILEQILQVSVTYVDSAMVGSL
                     GAAATAAVSVPTSTIWLVNGWMNAFAIGYAVLMARNIGGRRFERARMITRQALVTALI
                     FGAILSTVFTLIAQVLPKWIGAEEHVQTLARGYYTVIALAYLPNLLMITISSLMRLSG
                     DTRTPLLLNTLNNILNIILNMFFIFGEVDLGPITIHGLGLGVKGAAIATSLAVTITAI
                     LLFIIWLTRPSIIQLELNQSFALNREIQKNAFRLGLPVGLERSTLSFGQIVLTKIVGS
                     LGTTALAAHFLANTAEAMTYLPASGVSTAATTLVAQSLGRGDKTLAKRFGNATTLIGT
                     LLMTTMGVVLYFTAPYLMGFFTRDRNVIALGTTILRIEAFVEPAFGLSMLVFGVFRGS
                     GDTRRPFYISIAGMWVVRLPLALFMVRCTSLGLVGIWIGMASDLLLRGIIGLAMFLRY
                     GWLESSSDVH"
     gene            complement(33763..34761)
                     /gene="pta"
                     /locus_tag="PLF67_02245"
     CDS             complement(33763..34761)
                     /gene="pta"
                     /locus_tag="PLF67_02245"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOE88867.1"
                     /translation="MLFAEKMKAQAKAAQKRLVLAEGSEERTIKAARIIADEHLASEV
                     ILIGSKAEIEKAASASSTSLEGLTLVDPATSDRRKQYATEFYELRKHKGMTEEQAFED
                     IANPLRFGAMMVRLGAGDAMVAGAENTTAAVLVAAFNIIKTAPGISFASSCFVMATDK
                     KEYGADGSFIFADCATIPNPTAEQLAEIALASAASCSTFLGVEPKVGMLSYSTLGSAS
                     GELVEKVVEATKIVREKNPSLAIEGELQLDAAIVPSVAASKAPNSKVAGKANTLIFPD
                     LQAGNIGYKLVQRLAGAEAYGPILQGFAKPISDLSRGCSVEDIVVTSAITLAQAAK"
     gene            34856..35605
                     /locus_tag="PLF67_02250"
     CDS             34856..35605
                     /locus_tag="PLF67_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOE88868.1"
                     /translation="MSEKNRVGVYGLGRFGLFWATRLAEKGFEVVGCSRRTVELPPNV
                     RQVSVEEVLDSPTVFYCVSISAFEEVLANSLPYLKEGAVVMDTCSVKLYPARVMKRLL
                     PPTIQAIATHPMFGPDSGKDGLEGLPFVMDNVSAHEETWRWWVDEFTSWKMDVLMMSC
                     DQHDREAAWSQGITHFIGRTLAELNLGSTRLATKGYQTLMGVVEQTCNDPLQLFYDLQ
                     RYNPYTKQMRMGLRGALVTTMEALKEQEEIL"
     gene            35602..36903
                     /gene="mtaB"
                     /locus_tag="PLF67_02255"
     CDS             35602..36903
                     /gene="mtaB"
                     /locus_tag="PLF67_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOE88869.1"
                     /translation="MRVCVYTLGCRLNQCESEAIADSFAREGFEIVGEQDGADLYIVN
                     TCTVTSKAEQKARRMIRRFAQSSPTLVTGCYAQLNREELESLGKEVLVVPLDGKARLL
                     KLAAHLQVAMESSFSLFEGMKSFTGEDATVFDYAAATFSYHSRAYLKIQDGCDNSCAY
                     CRVHVARGKAQSLDRAIVIERAKALEEAGFNEIMLTGVNLTMYDHRNEGLGGLLEALL
                     GEIGDLRIRLSSMEPDHIDERLLGALEDPRMQPHFHIPVQSGSDSVLKRVGRKYTIAE
                     LTAILDRLKTVKDDPFLAADVITGLPGEGEREFEETYMFLKEIGFSALHVFPFSPRPD
                     TPLFNAKDRVPESVRDERAKILRTLSAELTDAYLRRQEGKQGEVIVQGRKGGFWCGVT
                     GNYVDVRIVNPPLFAQRGMLLEGVFGKKTDRFVEFIVTETS"
     gene            complement(36887..37804)
                     /locus_tag="PLF67_02260"
     CDS             complement(36887..37804)
                     /locus_tag="PLF67_02260"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOE88870.1"
                     /translation="MEPCKDHPFDSTVFIPSFLKTFDRIAGKANFHGLKPLPRMEIVG
                     QLCDELLELFFPGRCGREQARMDLEEYLAYQMDVVTSHLKGQLYLAFRYDDETLGSEA
                     AEERSDRAVRSIASRFPSIRLSLKADAQAAYEGDPAARNIREVILSYPCIKSLTIHRV
                     AHELYRLDVPIIPRMMGEYAHAQTGIDIHPGAQIGSSFFIDHGTGTVIGETTVIGSNV
                     KIYQGVTLGALSFPRDACGALIKGAKRHPTIEDDVTIYANATVLGDITIGRGTTIGSN
                     VWIKESVAPNTLVTIGEPEIRKREIRKSQ"
     gene            complement(37834..38349)
                     /locus_tag="PLF67_02265"
     CDS             complement(37834..38349)
                     /locus_tag="PLF67_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOE88871.1"
                     /translation="MNERRILLVAFFTALIIVGSFIRIPLPPVPITLQTLFVLLAGYM
                     GGPQVALMSVGIYLFLGAIGLPVFSGGGGFALLFGPTGGYLLALLPAALIAGFGGFKR
                     IWRMVLFGILATIVIYLGGVSWLKHLLSISLSQAIAVGMLPFLVGDGIKLGLAIALAR
                     RFSERVRSLLA"
     gene            38432..39388
                     /locus_tag="PLF67_02270"
     CDS             38432..39388
                     /locus_tag="PLF67_02270"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOE88872.1"
                     /translation="MSRKTEVLARLQQALNPLSGERIASDLSISRAAVWKAIEGLRKD
                     GYLIEGVPNQGYRLSSVQPLLNQSKLFSLLPSYRIEGVDAIDSTNSELKRRNPKTKTL
                     LFANTQSGGRGRLGRSFFSPRGGVYFSLALPLNVSIDSALLVTSIAAVATAEALSEIA
                     HVETSIKWVNDLYLGDKKLCGILTEGIISMETKTLATVIIGIGINLHLPIDRFPAELQ
                     AIATSAFDSERPLSIDPNRLVQGIVTNIERLIEDLPDRSFLEIYRRRSNLIGKTVQVH
                     EGVHSHPARVLGIDDDAHLLIADETGRTRSLSSGEVSIRLEG"
     gene            39428..41155
                     /gene="argS"
                     /locus_tag="PLF67_02275"
     CDS             39428..41155
                     /gene="argS"
                     /locus_tag="PLF67_02275"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOE88873.1"
                     /translation="MLRAIRAGWKARIEEELYKIAETNGKVSLPELIVQTPPKVELGD
                     IAFPLFAQAKVLGMSPPALANELKNRLDAQELLVSGPYLNVRIDMNALALDLTKAIRK
                     EGDTYGHNRTLGGKRIVVEFSCPNTNKPLHLGHLRNDSIGESISAILKANGAEVRKVN
                     LINNRGVHICKSMLAYQKFGNGETPESAGKKGDHFVGDYYVLYNRKAEEDPSLEGQAQ
                     AMLKAWEDGDEEVVALWEKMNRWTLEGLSQSYERMRVSFDHYYYESETYRYGRDEVLK
                     GVEQGVFYREADNSVWVDLEAIGLDKKVLLRSDGTSLYMTQDLGTAIRRYEDWFFDSM
                     IYVVASEQQYHFKVLFHALGLLGYPWAKDLHHLSYGMVNLPDGKMKSREGKVVDGDDL
                     VEELTLLAKEEIIAKEREELVGDLEETSLSIALGALNYYLLQVSPTKDMIFNPAESIS
                     FNGNTGPYLQYMGARISSMLAKYEQEIPASFDASVLTLEDERSLIRQLALFGEVVERA
                     AEGYDPSQITAYLYELSRTFSRWYHDNPVLKASTPALVEARVVLSSNVLQVLKNAFAL
                     VGIPFLAAM"
     gene            complement(41148..41705)
                     /locus_tag="PLF67_02280"
     CDS             complement(41148..41705)
                     /locus_tag="PLF67_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE88874.1"
                     /translation="MKEQLILSMLSLASEQGLGNVSLSRLANENGISKATIFHHFTGR
                     AHLIEEVFAYCHRLALKRMRTIDLEGNAEEVLLRALDHWSELYETEPLYSFYRIVEQE
                     SWTNGQARTIKQTFDEMLIAQSSVLIEHLEERGKLRIDDLELAIETFSATIARHLVRT
                     LLTDEEIRYQAERFIRRFCTHYAPT"
     gene            41849..42163
                     /gene="rplU"
                     /locus_tag="PLF67_02285"
     CDS             41849..42163
                     /gene="rplU"
                     /locus_tag="PLF67_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOE88875.1"
                     /translation="MYALVEILGKQYKAIEGETVQVDYISKQEVGSAIEYATVLAVVD
                     EKNARFGTPYVENAKVKVTLVENIKGDKIRVFKKKRRKGYRKTQGHRQWYSLVTIDKI
                     IA"
     gene            42176..42427
                     /gene="rpmA"
                     /locus_tag="PLF67_02290"
     CDS             42176..42427
                     /gene="rpmA"
                     /locus_tag="PLF67_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOE88876.1"
                     /translation="MASKKGGGSTRNGRDSKAKRLGVKRYGGQLVKAGEVLVRQRGTK
                     FHPGENVGLGSDYTLFAKEAGHVLFHSRRNRKYVSIVTE"
     gene            42449..43519
                     /gene="obgE"
                     /locus_tag="PLF67_02295"
     CDS             42449..43519
                     /gene="obgE"
                     /locus_tag="PLF67_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOE88877.1"
                     /translation="MIGFSDETYIDVASGDGGNGAVSFRREKFVPRGGPDGGDGGRGG
                     DVVFVVSHNLRTLAHLKRQRSFRAERGQNGSSARKYGRDGVDVVIPVPPGTVLKDPES
                     GEILVDLTDHERYVFLKGGRGGQGNWHFRSSTNQAPRYARPGQKGVALRIGVELLIIA
                     DIGFVGFPNAGKSSLLNTLTNARSAVADYPFTTKVPHLGMMRHHERDIVLADIPGLIE
                     GASDGSGMGIKFLKHISRTLGLAYLIDLSDDRYLTAFDTLTEELRAFDPELVGKERVI
                     IGTKIDEEGTDVRLAELKAKYPEETVIGLCVYFNEGVDAVKEAFYRIVERVDPATVED
                     KGFQAEMDMDAHYEMEKAPWEE"
     gene            43519..44145
                     /gene="nadD"
                     /locus_tag="PLF67_02300"
     CDS             43519..44145
                     /gene="nadD"
                     /locus_tag="PLF67_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOE88878.1"
                     /translation="METAMVGGSFDPIHRGHLHLIHTVASRSPYRRFILVPVSQNHFK
                     PDADPAPGSDRVAMANLAVEAYPEIYPDDPKLEFVVDDCELHREGPSYTYDTVRELYL
                     KYPIKGRLGLLLGDDLLAQLKKWHRYKELKELVRFIVIRREGATVEFDDIAADLFYLD
                     NPTVDESSTIIRTALKRLNADEPLDPAVERLMTANVASYVRTHRLYRA"
     gene            44114..44677
                     /gene="yqeK"
                     /locus_tag="PLF67_02305"
     CDS             44114..44677
                     /gene="yqeK"
                     /locus_tag="PLF67_02305"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOE88879.1"
                     /translation="MSGPIDFTALEAHLTPKRLRHSLAVTALCLRLNERYRLGLTDDD
                     LQAVGLGHDIARSWSEERLRSYIVKHEIALYPGEEAVVDLLHAPVGAHLLKTMGYNKD
                     VVQAIRYHTLGSIEMGALGFVLFASDYLDPNRTFLTEEDRKTLLEEPTIEALVFRILE
                     RNVRYLDAEGKQIFEPTRELLAVLGGK"
     gene            44674..45156
                     /locus_tag="PLF67_02310"
     CDS             44674..45156
                     /locus_tag="PLF67_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88880.1"
                     /translation="MRAVVVFSISLCVLLLGGCFAESTETTRYESETFNAYVWVEGRD
                     LAVVLVDRPLVVPTERTVMPDQDALDRIIGILQTLVEVSESSDPVSALLENRKALRKS
                     PLMDTLALIGDDPHAATRLRRLNTTTFLDLRGKPMKEDVLSVYLSDVRTYQMRRKSAQ
                     "
     gene            45153..45485
                     /gene="rsfS"
                     /locus_tag="PLF67_02315"
     CDS             45153..45485
                     /gene="rsfS"
                     /locus_tag="PLF67_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOE88881.1"
                     /translation="MNERTLSDAQAIVSYLEDHRCSDVHMIDLEGRASWTDCFIIATV
                     NSVGHLRGVVHNIWDLFNERGIAVANRQKKPAGDGWELIDCGDIIVHLMSEELREFYA
                     LERLWREL"
     gene            complement(45482..46843)
                     /locus_tag="PLF67_02320"
     CDS             complement(45482..46843)
                     /locus_tag="PLF67_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOE88882.1"
                     /translation="MHNRSRSLENGQFFESGFLFKLIIPLMLEQLLNVSIGMADILMV
                     TTVGEAAVSAISLVDSITILIVTLFGAFATGGAVVASQYLGKKDDESANTAAGQLLNL
                     TVLIATLLLLLCMPIRRGIISTIFGKIEPRLLDYGSTYFVYILLSLPFLAAYNSGAAL
                     FRSMGNSRVSLYISVVMNILNIGGNAFFIFVMKMGVAGAGLSTLISRIIGSILVLGLL
                     RNPRNRITIRSYNIFHIKGWMIRKILFVGVPTGVEGSLFQVGKLLVQGFIASFGTASL
                     AANAIAGSVSTFINIPGGAISMASVTVIGQTIGARRTDLSVFYAKRLLLFAYIAMFIL
                     ATPIFFLAPTIASFFNLSEEATKIATVLFQSSMVVSVFFWSSSFVLPNFLRAAGDAKF
                     TMIVSIISMWAFRVGGSYIMAMNFGWGVYGVWIGMYIDWVVRSIAFIRRFARGKWKEI
                     QVI"
     gene            complement(46897..47688)
                     /locus_tag="PLF67_02325"
     CDS             complement(46897..47688)
                     /locus_tag="PLF67_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE88883.1"
                     /translation="MLKLENITKVFYPGTVNEKTALIDINLQINKSDVVCIIGSNGSG
                     KSTLFNIIAGTYPVTSGTITFDGVDITNKAEYRRAMTIGRIFQDPTKGTAANMSVEDN
                     MITAETKGMKRLKISLNNAKREEFREILKQIGLDDRLKDNVGLLSGGQRQALTLLMTA
                     MSRPKMLLLDEHTAALDPRNAQIVMDLTERFISEQKLTALMVTHNMQFAIDFGTRLIM
                     MDEGQIILDLSGEEKKDLTVDKLVRLFKDIRKKTYATDAALLTTD"
     gene            complement(47681..48616)
                     /locus_tag="PLF67_02330"
     CDS             complement(47681..48616)
                     /locus_tag="PLF67_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88884.1"
                     /translation="MIEGIFVDGLIFSLMVIGILISYRILDFADLTCDGSVATGAAVA
                     TMAIVGGVPISIALVFAFISGVIAGMITAAIHNKLKIPGLLAGILTMTMLYSINLRIL
                     GNKANVPLLRVQTLYTKLPAFFSFFQPEIASLVGTLLVVLVIKSLVDLWFRTDLGVSM
                     GAMGGNEQMVISQGINPEVLKLMGIGLSNGLIALSGGLLAQYQGFADANLGQGMVVQG
                     LAAIMIGEFLFSSNRISLITLRAIFGAIIYKALLFFGRKYGYLVNITPNDFKLLTGIL
                     VIVSLFIAQGRSAASSRGARKKALARKNVQEEIDA"
     gene            complement(48673..49662)
                     /locus_tag="PLF67_02335"
     CDS             complement(48673..49662)
                     /locus_tag="PLF67_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88885.1"
                     /translation="MKRILTTLVLFVVVTTILFAAGQSDKAPKAEYTVGISKLIAHPA
                     LDAAEQGMIDYLTSQGLSVSFDRQNANGDISTASSIAQKFKSDKVSVAVGIATPSAQA
                     LAQVFGPNQSTPVVFSAVTDSAEAGLVAKNIAGVSDKSPVEEQIKLLMDVTGAKRIGN
                     VYASGEANGVVLMEMAKAATEKFGGEFVSAAISNSSEVKMAAQSIIDRVDAIYVATDN
                     AVVSAIASIDDVTTKAKKPLFSADPSPIAGLNAMIAWGFDYYNIGVETGKVIEQILQG
                     TEAGSIGTVYITDPAKYELWFNLDTASQLGYTIPQSLQDAAAVLIKDGKHVSR"
     gene            complement(49824..50081)
                     /locus_tag="PLF67_02340"
     CDS             complement(49824..50081)
                     /locus_tag="PLF67_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88886.1"
                     /translation="MSRYRDEDELEDEEDRYFNDWEEEEEDDFDEREAMRFPIDDDEL
                     LDDEEDDELLDDGFMVDGDDSYEDELYEEDDDDFYYYDDDF"
     gene            50665..>50944
                     /locus_tag="PLF67_02345"
     CDS             50665..>50944
                     /locus_tag="PLF67_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88887.1"
                     /translation="MYVGIGGPKGYEQVTIREDVYIPATGGKKTRIVRKVGKLSDLMA
                     EDPDFLIKLKEEVRLETLALKEAEKPILLPLSVEQIEKPSDATPTFLFG"
BASE COUNT        11716 a        13718 c        14300 g        11210 t
ORIGIN      
        1 ggggaagaaa ggaatacctg cagtcgctcg cctatacgga tgctctgacg ggtcttggca
       61 atcgaagggc gttcgacgaa gagttgattc gacttgccaa gagcgattca aaagaaaaac
      121 aatacggaat cgtcgtgttt gacgtgaata gcctcaagca aatcaatgat gactacggcc
      181 acgccgaagg agacctcgtc ctcagagaga tcggcgagct tctcacctcg ctctatggaa
      241 cgttcgccac gatctatcga atcggcgggg atgaattcgc attgatatgc gagcaatgcg
      301 gtcaggagag gatgagagat tttgaacgat tattcaccga ggcgattgcg aagaagccgt
      361 ttaccctcgc gttcggatcc gctttatcat cgcttgaccg ttccgactcg agcgattccc
      421 tctacaagct tgccgatcga cgaatgtatc agcaaaagaa gacgatgcaa tacaagacct
      481 atcacaaatc cccacaaaat ccggcttcaa ttgacacgtc agagggcact cggtagactc
      541 gattaaaaag aggaaacggc atgagcaagc tctggcagaa agattatgac cttgattcgc
      601 tgatggaaac ctttaccgtc ggtgatgatt acatcctcga tcaacacctt gtcatcgctg
      661 atgctcttgg ctcgatcgcc cacgcccgcg ggcttgaaaa accggggatt ctttccgagg
      721 aagaaacagc ggaacttacg caggggctca tcgatatcat cggtcttcgt ctacaggatg
      781 atttccccat ccataagagt gatgaggact gtcataccgc aattgaagga tttctggtga
      841 agcgggtggg ggaggcgggc aagaagatcc ataccggccg ctcgcgaaac gaccaggtgc
      901 agaccgccct ccggctctgg atgcgggagt tcgcctataa gcttgcccat aagggcgcag
      961 cgctcgccac gatgctcttc gatttcgctg aaaaacatcg ggatgtaccg atgcccgggc
     1021 ggacccacat gcagctggcg atgccctctt ccgtcggtct ttgggcttca ggctacgccg
     1081 aagagcttgc cgatgaatcc aagtaccttc tttcgatcgc cgacctgctt gaccagagcc
     1141 cgctgggatc cgccgcttcc tacggcgtgc cgttggatct cgaccggaac tacacctccg
     1201 ctctgatggg cttttcccgg gtccaaaaca acgtgctcta cgcaaacaac agtcggggga
     1261 agttcgaggc gattctcttg gacgggtgtg attaccttgg cttgaccttg agtaaactcg
     1321 cccaggatct catgatcttc accctccccg aaatcggata cttctccctt cccaacgagc
     1381 tgaccaccgg cagctcgatc atgccgcaga agaagaatcc cgacgggctt gagctggccc
     1441 gctcccgctc gagcgtcatc tcctcatgcg catttcgggt caaatcgatc atccgttctc
     1501 ttccttcggg gtataaccgg gacttccagg acaccaaagg agcgctcttt gaaggagcga
     1561 aagcgacatg gctcctcttg ggaatctttg aagtgatgat cgaaggcctc atcgtccacg
     1621 aagaagcgtt ggtgaagggg tgcaagccgg agctgtatgc aaccgatctc gttctcaagc
     1681 gctcccttgc cggcgagagc tttcgggatc tgtacaggga agtcgggctt catctggaag
     1741 aggttctccc cggtgacccg tatgaagcgc ttcgggagcg gacaagcgtc ggtactgccg
     1801 gcaatctgaa catcgaccgt tcgcgtgccg gcaaagtcat tcgggaaagc gaaacccgct
     1861 tgaaggcgat ccaagcgagc tatgaagctc tcagtggttt gaagcagctc gaagttgttt
     1921 ctttttaagc cgtttccacg atcctttgcg aacgcttctg caaaaccggg gtgtgtttca
     1981 ccgcttggtg gtacgcttcg gcgatattgg tgcagtaatc gccgatgtgc tcaaggtggc
     2041 ggatcaattc aagaatcaaa agctctgctt ttacatctcc gaccccttca ctgaggcggt
     2101 cttggacctt gttccccaga tcggctcgca gggcgtttat ctgatcctca tactcgttcg
     2161 ccttctgcat ctccgctttg gtgagcgtcc ggtccatccg gtcgcgaacg taattcagga
     2221 actcctggac tataagatgg aacgtttcca tctccttcaa gatttcatcc tcatagttcc
     2281 atcccatctt gtagcgccgt tgactgagcc gcgccagggc atagcagctg tcggtcacgc
     2341 tctccagttc atccatcacg cggatgaggg agttcaacga ggaagcattg gtggtggttt
     2401 gggaatcttg gagcaggtga acgatgaagt ttgaaagctg ttcctgcatc tgatcggcgt
     2461 actcttcatc ccgtctcatc aaatcgacca gatcatcaat cgcattgttg ggactgtcaa
     2521 acatcgaacg ataccgagcc agcatggtgc acgcaaggtt agccatcttt ttgatctcat
     2581 ctcggatcgc caacatatag atttcggggc tgtcgatgaa cggtgctccg atatatctga
     2641 agtgatacgt tccttcttca tactccgcct tctgcgggaa caaacgttcg ataagccggg
     2701 cgtatagtgt tacgaagggg aacatgagaa tcgttttcga gactgcggcg accgttgcga
     2761 acatcgcaag gaacaccggc aatgcttggg caagggcatc gccggtgagg atgaagacat
     2821 ccccgggaac gatccaattc accaatgcca gcagtggacg gaagaagatc agagcaagag
     2881 aacttccaag gacattgaag aggatatgtg cccaagcggc ctgtttggcg gtggtggagg
     2941 ttccgatcga tgccaaatac gcggtaatcg tcgttccgat catggcgccc agaatcatcg
     3001 ccgcaccctc ttcaacgccg acccaaccgt tgtatcccat tgtaaggatg attgccgtcg
     3061 tcgccgagga tgattggatg atcactgtga ccaccaagcc gaccagaaga caaagaaagt
     3121 tggtccaaaa ggtatttgcg ttgaaggtgg caagaaaggc gagagcttgg gggttgtccg
     3181 aaagactggg aatcgagttt gacatggagt tcaacccgag aaacagaaga ccgaatccca
     3241 acagtacatc acttttgtca cggtttcgac tccgcttgct gaacaacagc ggaagggcaa
     3301 acgctatcga tgtaagagca agaagggata tattcatctt gaatcccagc agtgccacga
     3361 tccatccggt aaccgtggtt ccgatatttg ctcccaggat taccccgatt gcctggacca
     3421 attccatcaa gccggcgttt acaaaactga cgaccatcac ggtggtcgct gacgatgatt
     3481 gaatgataac ggtgatgatg acgccggtca tgatcgaaat gaaccggttt tttgtcatca
     3541 tgttgagaat ggctttcatc cgatttccgg cggacttctg caatccctca ctgaggagct
     3601 tcattccata caggaaaagg cccagacttc caattatctg gaaaaagatc atgactgctt
     3661 tcataccggc tcaaccctaa caagtttaac ccaagaggta aagtaggaga ccgtcaacgg
     3721 ttcagtgtgg attgaacttt aaaagccctt gttgtatggt tgggctatga gcatcatcaa
     3781 accgcaaaag cttggaatca tatcggggcc gggctcggag tattttaccg gaaaagttgt
     3841 caagcatctt cggaggctct atttggaacg gtactacaag ctttccagcg ctcttgccaa
     3901 gcggcacggc atcagcgagg aggagatcct taaaaccgtc accctcaccg atgacctttc
     3961 caataaacgg attccccgct caaaggcccc caccaccttc caatgccccg atttcacgat
     4021 caagacgaag tatgtgcggt ttgcaaacgg tgaagtgaaa gccgagattc tcgatccggt
     4081 gcggggtctc agggtcttca tcatccatga cctttcgaac aatgaaccgg tgcgagtcag
     4141 cggatccgac gagcccctga ggcttacgat aaacgaccac ttggtgtttt tattgaccac
     4201 gatcaatgcg ctcactctgg ccggggcgga ttccgtaacg ctggtcattc cgacctatcc
     4261 ctattcccgc cagcataaaa agagcacgcg ggaagcgctg accgcctcga tgttcggccg
     4321 catcttcgag atgctcaagg tcgaacggat catcacgctg gacatccact cccgggagat
     4381 cgagaactcc ttcggacacc ttcatctgga gaaccttcac gcgtcctatc agaccctcat
     4441 cgctctgcgc aagctcgttg atttcagcga tccgaacctg gtggtggtcg ccccggatac
     4501 gggggcgatc agccggaaca agttctacgc ccaggcgctc caccgcccct ttgcgatgct
     4561 ctacaaggag cgcgattact cggtggtcag caaggacgcc cgcaactcca acatcaaatc
     4621 gatcaacctg ctgggagacg tcaaaggcaa gaccgtcctg atcgccgacg atatgctggc
     4681 caccggcggg acgatgatca tcgcgatgcg cgagttgacc aacctcggag cggagaagat
     4741 catctgcatg gtcagtcttc cgtttttcaa cggaaccgcg gtggaggact tcgaccaagc
     4801 gtatagagaa ggcatcttct atcggatcat cgggacaaac gccgtgacgc acggccgcga
     4861 tctgctggat aaagagtggt acatccaggc ggacatcacc gagctctttg cccgggtgat
     4921 cagccgtctt caccacggcc gctcgatcag cccgctgttg gataaccgcc ggttcatcca
     4981 gattctcatc gacaacactc aagcaaatcc aaaagctcct cttccggatg ctgccgctac
     5041 cgacccggat caagaagatt aatctttgat ccgggtgagt cagcagcctc ccttacgggc
     5101 ggtaaccatc atgccatagg agtgaccatc atgaagatgt ccaaccgaat ttcatcattt
     5161 caagcttccc cgatccgccg gctttcgccg tatgcccgtt cagccgtaaa gcgggggatc
     5221 accgtatatc acctgaatat cggccaaccc gatatcaaga ctccccaagt cgttctggaa
     5281 gcgatccgga gctacaacga gccgatcatc gcctatggat ccagcgaagg atatgagcgc
     5341 ctccggaacg cgcttcccgc ctattacgca acatatggag tggatatcaa gccggaggat
     5401 attttgatta ccaccggcgg gagcgaagca ctgctcttta cgctgatgac actctgcgat
     5461 ccgggcgacc aggtgatcat ccccgaaccc ttttatacga acgtctcctc ctttgcccac
     5521 tcggcgatgg ttgagcttgt tccggtgacc agccgtctgg aggacgcatt cgcccttccg
     5581 ccgatgaaag atcttgagaa gctcatcaac cccaagacaa aagcgattct tctgtgcagt
     5641 ccgaacaacc cgaccggtca tgtgtattcc aaagaagatc tggagcgaac tctccaactc
     5701 tgcaagaagc acgacatctt tctggtcgtt gatgaagtct atcgggaatt ctgctatgac
     5761 ggggaacggt tcacctcgat cctgaccttt gaagagtacg ccgaccgggt cgtttgcgtc
     5821 gattcattct ccaaacgcta ctcgatgtgc ggagcccggg tagggatgtt ggtgagccga
     5881 aacaagcagt ttcttaccga tacgctcaaa ctctcccaag cccggctgtg tccgccggat
     5941 atcgagcagg tcgccgcagc ggcggcgttg accaccgatc cctcctatct ggtcgaagtc
     6001 aaaaaggagt atgaaagcag gcgcaacttc ctcgtcgagg cgctcgagaa ggtcgaaggg
     6061 gtgaagacca ccaagcccaa gggggcgttt tacatcatcg ccgagcttcc cgttgaggac
     6121 gccgaggatt tcgccacgtt cctcctcagg gatttcgatc tcaacggaga gacggtgatg
     6181 ctcgccccgg cggaggattt ctatgcgacg aaagggatcg gtcgaaggca ggtgcgcatt
     6241 gcgtatgtat tgaataaaga agacctcggc aaggcggttg catgcctcgc cgccggcttg
     6301 aaagcatatg ctgagagagg cgcttaagag ccgatgatct tgatcaagac tcgtttatcg
     6361 cgcagtccgt caaattcgta atagaagatt tgctcccatg ggccgaggtc cagttttcca
     6421 ccggtgatag cgacgaccgt ttcccggccc atgatcgttc gcttcaggtg cgcatcagcg
     6481 ttcttctcct tttccgtatt gtgccgatag cgctcgtagg ggagctcggg ggcaagttct
     6541 tcaagccatt tttcaaaatc ttcgtggagg ccttcctcca cggagttgat gaatacactc
     6601 gctgtgatat tcatcgcatt ggccagaaca agcccttcct tgattccgct ttcagtgacc
     6661 gcctcaccaa tcgcctcggt gatgttgacc agacctcggc gtttattgag gtgaaaccaa
     6721 agctcttttc gatatgtcac catacaggcc tccttctgta catccagtat atcatggagt
     6781 ttgtcacaaa acctcaggta tgctatgctt ggattcagga gagaaggcgt gaaaacaaac
     6841 gagagttggg atcttagttc cgtcggcgat atttcagccc aaaagatccg gcggaacctt
     6901 cgcgagctcg cggttttttc agcaaattcc attcgttccc tgaaagagac ggcgtttcga
     6961 tatgaaccga tcgcattgat ggagcaagct cttgagcact accaccgaac cgtcatcagg
     7021 gaatggaaag gcgatggtga agcgattcga atctcaaagg cgcttatctc ctatcttgcg
     7081 aatcttctac agtctttttc aaagccgctt tcaaagaaca ggcaggtcaa acaaaaggat
     7141 tggaaagata tcgttcgact ctttgatgac atcgtccgca aatcgatccg catcgtggat
     7201 aataccgctc ttgccctgaa ggcgaatgga acgctgaccg atccgatcct gcttgagcgc
     7261 tttcaaaagg aggcgagcgc atgggtgctg cctccgttgg tcgatgaaga gctcttgaaa
     7321 cggcaccatc gctctctgct ctatcagctt caaccctaca acgccctgat cagtgaagtg
     7381 tttccatctc acctcgacgg agtgcttcag gcattgtcga ccctgatcga aacggacgag
     7441 catgacctgt tgaaccatac cgcgctcagt gaagcggatg ccaaggtgct ggctcgtccg
     7501 atcgcaagcg aagagtttcg agtcgacaac catcgcctca cgcagttcca acaatcctcg
     7561 ctcaagccct tcatactcat caacggctcc tattactgct tcgatcccga acgagtcctc
     7621 atcgaaggct atggaatcat caaaggggcg gtgaccacct caagcggcga gcgtaaagag
     7681 gcgtgggaga ggattgagaa agagaagcgg agcctgcttt cggtcacctt cttcaccgca
     7741 atgctttctt cgatgcacta cacgagaaag gtggagtata aggacggttt tctggacgcc
     7801 ctcttcgaag atgggaagaa ggagctggtg atccagattc ccgccgccca caccgtcttt
     7861 cccgaggatc cgatccaaga aagcgctgcc tatgctcggg cgttgatcga agaacagaag
     7921 gcgatcgact tggcgaaaac ccatcccgcc ccctcggtca tcatcgattt gagggcaatc
     7981 aagagctatc cggtaacatt gattgataac gcgctcaccc tttccttcct tcaggtggcg
     8041 gcgttggcga caacgtggga gggggccaac gagatcaaag gtgagctggg cctgctgccg
     8101 cttggcgatc ttacgccacc tccccacgat aaagagccgg aagaacaatt ggaagctgaa
     8161 cagccgaaag ccgggcaaac agatgatgaa catgatccga tgaaggagtt tgacgagcat
     8221 ctggaagacg agctggaatg ggaagaagag gaagatgaag attcctttga cgatgatgaa
     8281 atcctagaag aagagctcag cgcgatcgat gaggaagatg agtacgaaga tgatcttgaa
     8341 gagtttgagg gtgatgagca ggatgaatat accgcgatcc ttgaggatga tgaatacgaa
     8401 ggtgaagctc ccgaaccgat agaggaagga aaggaggagc cggaattcac cttggaagcg
     8461 gaagtcgatt cctcctggca gcgttttgat caagggttca ctctgggaat caaggatccc
     8521 cggatcatcg aaggggaata cctcgactac gccgatgaag aaggcgagga agaggaggat
     8581 gactgggcgt acagcggatc atcctatttt acttccgcaa accccttgga agactatctg
     8641 ggtgaagacg agtacgagac cgccgatgaa ctgttcagcc aaagcgaaca gccggttctc
     8701 ttcgacgtgg aggatgaacg gatgttcgat cctttttatc gcaccgacct tgagcggcaa
     8761 gccgttgaag aggattacga aagcgaagcc atcgggcttt ttgagtatga aagcgaaggg
     8821 agcggatatg acgaagagga ggttcttgta ccgtcatcgt ttgtccttgc cgatgaagag
     8881 atggaggaag gcaaggaaga cctgcttcgc taccacaccc agtatgtcga ggatgacgat
     8941 ggagacgaat tcggtttctt ggatcagatc caaaggagcg ccgaacagca aaaacgcccc
     9001 tcttcccgtt tcactttgat caacgcggtc gaacatgcga aacatcaacc ccctcctcaa
     9061 gaggaagaag ataaaaaagc tcctccagcc ccaccgccgg cagcaaaaca ggcttccgtt
     9121 gaaccgtttg agttggatct gccgccgaac ctcagtgaaa tcttcaccct tcttgcaaaa
     9181 aagaatcagt ggtccttcaa tacctacctc aaagaaagca tggctcatga gatcgattca
     9241 ctggagcgaa tgctccaaca gattcgggac acgactctcg aaccggggca ggatcggatg
     9301 ttcacgatcg tccccatgga gctgaccatt attgttttgg cggcgaaagg ggatccgctc
     9361 agcacgtgga cgcgaaaagt aaacgttgcc gccctgatgc acgccaacaa aaaacgctcc
     9421 tggcgcgccc tttcgatcgg atacgaccga aagagaaatt tggtgtttgc cgatgattgc
     9481 ccgatcaggc gggaagattt tacagcaacg gattggaagt ttgtaatcaa tgcggcgaat
     9541 cggctgcggg ataaaaagcg agtatagaga gcatatgaag acccacttgg atgttgccct
     9601 gcaggcagct cgcagagcag gctcctacgc cttgggagcg gagaggaacg gagcgatctc
     9661 cgttcggatg aaagaggcga acgattacgt taccgatgtc gatatccaga tcgaacagct
     9721 gatcaaagac acgatccggc tctcctttcc tcatgatgga atattcggtg aagaaggcgg
     9781 tgaaagcgga aaccttcaat ccaaccgctg ggtgatcgat ccgatcgacg gaacgacgaa
     9841 tttctttcgg gggatgccca actacacgat ctcaatcgca tgggaggagg aaccattcca
     9901 cccgctggtc ggggtcgtct tcaacccccg tcacaatgaa ttgttctggg cggcaaaagg
     9961 ccagggagcg tttctgaacg ggcagaagat caccgtctcc tccgtccggg agctgaatca
    10021 ggcgatcgtg gtgctggttc cgcctcaccg caggcgcgac ctcgccgatt cctactttga
    10081 aacgatgaac acgatcttca aagccaccag cgatatccgc tccttcggat cctgcgcctt
    10141 ggagctttcc tacatcgcaa gcggacgggt cgacggatac tatgaacggg cgctgggcta
    10201 ctacgatatg gcagcgggga tgatcattct tcaggaagcg gggggaatcg tcaccccggc
    10261 gtttgaagat gctcctttca gcgaccgctc ttgcgacatc ctcgcttcaa acgggcacct
    10321 tcatcgagct ttgagctcct tgatcgcttg atgaaaccgt ttctttcccg tagggtcttg
    10381 gcatgaagtt caagctggcc atctttgatt tcgacgggac gatcatggat acctccccgg
    10441 gtatcttctc aaccgcgaat gcgacgattg aaagcctcgg gttggagccg gagaaggatc
    10501 cctcggtcct cgcccggttc gtcggcccgc caatccgcga atccttcgtc gcggtgtaca
    10561 acctcgatga atccctcctt gatgaggcgg ttacgcgata tcgggcgatc tacgaccgga
    10621 cgggacgctt tggagccgag ctctatgaag ggatcgaccg gatgctcgcc gcgctgaagg
    10681 ctgccggcta ccgcctcgcg atcgcgaccc tgaagtcgga accggtggtg aagcagatga
    10741 ccgaccactt cggtctcacc ccctatttcg attcgatccg cggcagcgat ccgatccata
    10801 aaaggagcaa ggcggcggtc atccatcagg tcttggagga tttgcaaata aaagcggaag
    10861 aagcggtcct catcggagac accccacacg atgaggaggg agccggggaa gcgggcagcg
    10921 ccttcatcgc cgtcgattgg ggattcggct ttcccaaagg atatcgacca tcatcgacca
    10981 tggtcgctga aagcccgctt aatgtattaa cgttcctctt gtaagaggct ttgggcctcg
    11041 atatggtaga ggttaaggag attatgtaat gtcgtttcaa gtaacaaaga gtgaagttgc
    11101 caccccgaac gccccgaaag cgatcggacc gtacagtcag gcggtcagtg ccggaccgtt
    11161 cgtattcacc agcggagtgc tgccggtcga cccgaaaacc ggcaagctcg tggagggcgg
    11221 aatcgtcgaa cacaccaaac aggtgttcga gaacctcaaa gcgatcttaa aggaggcggg
    11281 tacgagtctc tccaatgcgg taaagaccac ggtcttcatc gccagcatga aagatttcgc
    11341 agcgatgaat gaagtctacg cctcctatct gggagacggg gtgaagccga gccgatcaac
    11401 ggtggaaatt ccaaacctcg ccctcggtgc attggtcgaa atcgagatga tcgtgctccg
    11461 gtaggaggga aagatgaacc ggactccgct ttttgagcac tatggcgaac acgccaaact
    11521 tgttgatttc ggcgggtggg agatgcccct ccactttgag ggggggattc tcgccgagca
    11581 tgaggcggtg cgaaaagccg ccggcctgtt tgacgtttcc cacatgggcg aatgcgtcgt
    11641 caccggcgag gaagccggtg cgtacctgga ttggctgctg accaatcggg tcagcgacat
    11701 ggaggtcggg cagtgcaggt atacgatgat gtgctatccc gacggaaccg tcgtggatga
    11761 catcctcgtc taccgcaggc acagtgaatc ctacatgctg gtgctcaatg cggcgaatac
    11821 gaaaaaagac ctcgattgga tcaccaagga caatcccaag gccgaccgtg ctccgttcgt
    11881 cgtggatatc agcgcttcaa ccgccctttt ggccctgcaa ggccctcaag cggccgggat
    11941 cttgagccgg ttcatcggcg acatcgatga ggtggaaggc tttaccttcc ggagtcaatg
    12001 cacgatcggt tcgattctct gcctcgtcag ccggaccggg tataccgggg aagacggctt
    12061 tgaaatctac tgtgaccggg atgatggacc gaagctgtgg agcctgctga tcggagaagg
    12121 ggcggttccc tgcggattgg gagcgcggga caccctccgt ctggaagcga agctgccgct
    12181 ctacggtcag gagatcagcg attcgatcac tcctttggag gcgaacctcg gagccttcgt
    12241 gaaattcgag aaggaagact tttgcggaaa agccgccctg ttggagcaga agaacaaagg
    12301 ggtgccccgg acgctccggg ggatcgagat gatcgacaaa tcggttcccc gcaccggcta
    12361 tccggtgttt cgggatggga aggagatagg atacgttacc agcggaatga agagtccaag
    12421 catggatatc ttctttgcgc tggtcttgat cgaccgcgaa ccggccttga agttcggcga
    12481 tgagcttgag gtggaaatcc ataggaaatt aaagaaggcc cgattgattc ggactccgat
    12541 ctatcagaga agtcattgat gtgctgaaga ttgaaactcg cgttcccgaa gggttcatcg
    12601 ccctcgacaa ctccgccctg atctatcctc caacggaggc ggcgttcaat gcgaatacgt
    12661 ttcgaatctc gatcgatctg aaggaagcga ttgaacccga gcgcctcaag cgggcgttca
    12721 aggatctttt gaagcgctgt ccctatgtca acgtaacgat gaagaagggg ttcttctggt
    12781 attacctgac tcccaatccg aaggatccga tcattcatcc cgagggttcc taccccgccg
    12841 ggcgctttct gttcagggag aataacggct atctgattcg agtcctccat gcaagccatc
    12901 ggatcgcctt ggagtgtttc catgcgctca gcgatggaat cggagttctt gagctgttga
    12961 agcttctggt ggagcgctat ctcgcttccg gtgggagaga ggtgtctttt cagggcgatc
    13021 tttcgttcga tgaaccggct caagcatggg agagcagcga tccttttcaa acactgtacg
    13081 acccgaggtt gcccggctat ccctcctacc cgagagcgta tcactacaaa gggtccggtt
    13141 catacgacga gcgggtgaaa gtcatcagcg ccaggatgaa gagctccgat ctcaagggcg
    13201 cggcgaagct ccatgatctg acgatcggcc aataccttgg cgccgtattc atccactccc
    13261 ttcaggagct tcagctcaaa gaggggagga agatgagaag gcgaaaaccg attcgcatct
    13321 ccgtcccgat gaacctgaga cgcctgttcg atctgaagac gatgcgaaac ttcacgctct
    13381 tcgcggtagt ggggatcgaa ccgaaccgag gatattactc ctttgaggag atcgcccgcc
    13441 atgtccggct ccaaacggct cttgcacagg ataagaaggc gttgctcagt caaatccgac
    13501 gcaatgtcgc cggcgagcac gccttggcgt tgcggttcgt tccgacgatg ttgaagaatc
    13561 cgtttttcaa actgctcagc gaccatctcg gggatcagca atacagcggg gtcatcagca
    13621 atctcgggca tgtggccctt ccggggctct tggaagagct tgtcgagcga atggatttcc
    13681 acctcagtcc cggattgctc aataaagtcg ttctttccgt aatcggatat aaggatgaag
    13741 tggtgttgaa cttttcctcc ttctttacta ccgataccga attggagcga atctactgtg
    13801 aacgcctgat tcatgatcat gtacctgttc atatagcgtc caaccgagga ggtctctgat
    13861 ggcttattgc gtccgttgcg gtgtgcagct ggccggtggc agcaaacgct gtccgctctg
    13921 tgacacaccg gttcttttgc ctgacgggtt catcgaagag atcgaacgcc ccttgttttc
    13981 caagcccctc gagcgagcgc aaaagggtgg cttgagcaag gctcgaaaag gcattttgga
    14041 gctgatgatc gccctcggcg tcgtcgcgtt catcagtgtc ggcctggccc tcggtctttc
    14101 cggccatcgc gacattgtgt tgatcccgct ggtcgcaatc gccgtctccc ttgtctcgct
    14161 ctcgtatgtg ctgatgggtc gacaaaccta cgttgctcaa tcgactgttc atttaacctt
    14221 gtcggcagtg ctgctcatcg tcatcgacgg tacgctcggg cggatctcct ggtcattgat
    14281 cgccacattt tcgatcgccc ttttctgggt tctgtgggta tttcctttca tgaagcaccc
    14341 cgaattggac ctccctcgga agttggcgac ctcaatggcg gcggttctgt tctacctcgg
    14401 cggactcaat cgagtgcttg atggcaagtt cacttggttc gttccgatcg ccctgccgct
    14461 ttggagcttc acggtgaccg ccacggtggt cttgcttacg agcttcgctg cccgcagagg
    14521 gcgaaccgtc acgatcaccg agctggtgct ctcgacgctt ttcatcgtct ttctcgccct
    14581 taccgggctg gatcttttgc agaatcatta cagaaacgga gcatgggctt tgcggtggtc
    14641 cgctccgctt ttgatcggcg cggcggttct tctcgtggtg ctgcttgcct acgtgctttc
    14701 ccttcgggtc cgccgctact ttaccagcag tcggactcct aggtgaaatc cgcccgtttt
    14761 ccgtcgacgg aacgaagcag atcggaggga tcgagccgga cctgcattcc ccgtttgccg
    14821 gcgctgacgt agatgaaggg aaggctgaac gccgcctctt cgatgaaggt gggaaagggg
    14881 cgtttcatcc cgatcgggga acagccgccg cgcaggtatc cggtgacatt ctgaatctcg
    14941 gtgagcttca ccagctccaa agctttgctg ccggtgaggg agcgggcttt tttcatgttt
    15001 atcgatcgat ccgccggaag gcagaatacc caatactgct tccgttcatt gacgaggaca
    15061 atcgttttaa acaccgtttt cgggtccaag cccacggtct gggcggcatg aatcgcatca
    15121 agcgcttcct catcaaagcg gtaggaaaca agttcaaagg gaatcttccc ggcctccagg
    15181 attctcattg catttgtttt catggtagca tcgtagccgg gaagccgctt cgaatcaaga
    15241 aaccgattga agcggccccc atggtgaaat acttgtaacc ggatccatgt ttcgggtgtt
    15301 ttatcttaga ggtcttgagt ttggagtgac cgtggtgcta tactacgaaa actcaacgtc
    15361 ttggaggtgt ggcaaacgat gaggaacgca aagcggtgtg cccttgtttt actgattata
    15421 atagctattg ttcatggagc cctgatcgct caggataacg gtctggtcat ccgttcgccg
    15481 ctctatggat cttccacaaa ggatcttgtc ggcacgacga tacagataca atcatatgcc
    15541 gagcagctgg gccttgctgc aacctcgacg ataccgaccg gggatgagcg gctcctcaat
    15601 gcgatcagcg agccttccta tcccgtaacc ccgggagaca cctaccgggt catctatctt
    15661 gacggattga aaacggtaac gctggatttg caggtcaatg aagcatatca ggtggtgctg
    15721 cccggtcttg gccgcgtcaa cgggcaaggg cttacctttc agcaaatgag tcaaaagatc
    15781 accgatctca tcgaagcgta tcacagctac tccaaccccc aagtgatctt caccggggtt
    15841 ggttctttct atgtctcggt gatcggggag gttaccggtt ccagaaccgt tcaggcatgg
    15901 ggccttaccc ggctctcaca agtcgtcggt gcggccaccc cctacacctc gacccgtacg
    15961 atcaccatca cccacgccga cggaagccgg aacacctatg atctctacct cgctctccgc
    16021 aaaggagttc ttacagaaga cccgttgctt aaaagcggag acgtcgtgag ccttggccgg
    16081 gccgagcggc tggtccacat cgacggtcat gtgtatcgaa cgggaagcta ccagcttcag
    16141 gagggggaga acctctcttc gttgatcacc gattacgccg gaggcgtcct ctcgggcggc
    16201 gatatccagc ggattcggat ccaacggttc gacgaggagc tgaacgactg gacgattctg
    16261 tacgcggacc tgctgaagga ccaacagttt gaagtgcggc accttgacaa ggtatacgtc
    16321 gacctgctcg ggccgatgcc gcaatcaatt ctcatcgaag gggcgatctc ctcgaccgag
    16381 gcggcgaata ccctcagctc ttcatcgttg accgggtatg tttcgggtcg aatcttctat
    16441 cagctctacc cgggcgagac gatcaagcac gtctttgaaa ccctgagcgg ccggcttctt
    16501 tcgatcagcg atctcgagcg ggcgtatatc cttcgtgacg gacagcggat caacctcgac
    16561 ctgcagcgga ttctctatgg gcaggacccg aactcgaccc gtactcttga gaacggcgat
    16621 gcgatcatga ttccgttcag ccaacgcttc gtctccgtta ccggcggggt ggtgcgctcg
    16681 ggaatgtacg cgtatgcacc gaacaagagt tccagctact atatcgcgct ggccggcggc
    16741 tacagcgatg acgcttcctt ccccctgtcg gtgaaagtcc aaggtgagga cggcaggaaa
    16801 atcgccaaaa ccgaagaaga agtccccccg agctcgacga tcatcgtcaa gaagaacacc
    16861 tttacgaaag atatcgcacc gactgtagcc attgtcggac tggttgccgc aatattggga
    16921 atcgtgtcca caacgttgag catcatcaaa gatgtgcggt cactttagga gcagcgaatg
    16981 aatcaggcat atgatgacca atatacagtt caccagcaat tcgatgaaga aggaatgacg
    17041 atcagagagc ttctctcgat cttccgcaag cggttcttct ggtttcttct cggcctcgtg
    17101 ctggtaagcg gcgcggcggt cgggtatctc ttctgggcga ctccgctcta cgagtctcag
    17161 gtttccgtcc tcgtcaatcc gattcaaaac tcctcatcga tcgattcgct gctcagcgtc
    17221 acctcctcgg gagcgaagat cgcgaccgaa gtcgagctca tcacctcccg cagcaacatc
    17281 aacgaggctt tggcgcggct tgatctcacc cggtatgaaa acgacgaagg tgaatcctat
    17341 gcgatgaaca tcgcgtttcc caactcggtg aaggagaaga tctccgtcac cacggtgaag
    17401 gacaccaaca tcgtccgcat cagcgtcacc gacgcaaacc gcttctttgc ccgggatttt
    17461 gccaatgttc tggctgagag ctatgacagt atgctcacct ccattgcccg gaactcgaag
    17521 accgctcagc ggaccttcat cgagagccag atcccgatca acgatcaggc cctcagagag
    17581 gcgaacgacc gacttggaga tttcaaggag cgaagcgaga tcatccagct ttccgacaag
    17641 agctcactgc tggtcaacca gatatcctac tacgaccttc gactcgagcc tcttaagctt
    17701 cagcttgaag aagcgtatat cggcttccgc tcccacgaac ctttcatcgc cgccagcggc
    17761 accacctccg atcaactgct gacaacgatc agaagcgacg aggagatctc aaagaagctc
    17821 agcgagcttg agtcgtggtt gctggagctg gcgatgtacg agtcgctgcc gacaaacctc
    17881 ggtcaaggag ccctttccga aggaacgctt gcccggattt tcaccatcaa caacgcgatc
    17941 gatacaacga agaagagtct tctgaatcgg gttcgaaagc ttatcgaatg ctgctacatt
    18001 gattcgctgg cccagaacat cgcccagatt ctcacgaccg gcgtgtcgat cgatattctt
    18061 cagggccgag ccgaggtgtt tgaagaagag ctttcccgcc tgccccttct ggagcgcacc
    18121 ctttccgaac tgcttcggga tgttcagatc agcgaagcga tcgggctgaa gcttcgggag
    18181 atgcttgagg agatcaagct tctggaagct tcagtcagcg gaaacgtgaa agtgatcgac
    18241 acggcgaact tgccgctcaa gccggtcagt cccaactccc tcctgattct ggcggtagcc
    18301 ctgcttttgg gttcggcgat cggactgctg ctcgccctgt tggtggagtc cttggataca
    18361 tcgatccaga atgaggaaca ggttcaacgg atcgtcggtc gaaacgcccc gatgctgggg
    18421 tgggttccga tcatgaaaac atatcaaaag gatgtgtatc cgatgttcat ggttcgtacc
    18481 catccgaact cgtttgaagc ggaacggctc aagctcatcg cgaacatgat ctattcgatg
    18541 gacggaaaaa aagtcttctc gatcaccagc agttcgatgg ccgagggaaa gagcatgatc
    18601 gtggcgaaca tctccctcgc tttggcccaa ctgggcaaca aggtattggt cgtcgacgga
    18661 gacttgcgcc tgccgagcat ggaacgcttc ttccgccttg aacaccagag tgtcgggttg
    18721 gtcgacgcgg ttaccagcgg agtttccttg gaggattgca tcgtccaacc gcttgaagac
    18781 gtaagcaccc tccacctgct tcccgccgga tcgtcaccct tggtgccggc ggcgattttc
    18841 tccaacccaa gatatatatc ctggacgaaa atgctttcca atatctacga ttacatcatc
    18901 atcgacgctc ctccgctcga tgcggcgagc gagctgcttg caatcgccaa acacgttgac
    18961 ggcttgatca tcaccgtccg ggcgggagtg accagccgta tcgctctgcg cgaccttctg
    19021 gaaagcctga tcaacgcgaa tgttcagctc gccggcttcg tcttcaacgg agtgatgcct
    19081 tcaaagcaca gccgctatgg aacatatggc tactcggttt cctataaaat gggaaagacc
    19141 gaacgaaagt tcgggcggaa atatcgctat ctccccagaa ggcgaaccgc ttcatggtat
    19201 cgcaaacggt acaaacagga cgtcaagatc cgcggaaaac tgccggcgct cttcaccccg
    19261 gttcgggcgt tcggtccgca cgctccctac atgaacctga aggaaatgga ggagaagaac
    19321 aatggaaacg gcgaagagaa actgatcttc aagccggttg tttccggaga tgcgggtagc
    19381 agatcgaagg aactggctga gaagcgtgat gaaaccggcg aagagaaacc ggtcttcacc
    19441 ccggccgctt tcaaagatac cccgaaacat cccatgaccg aggataaggt gattgacttt
    19501 ctttccgcga tcgagggaga cgaagcggcg cgcgggaaaa aataggcggc atcggatgga
    19561 cagagtcgct cctctttgat attgtacaga acagaaatga ggaggcttcg gatgaaggca
    19621 catgtactct accgtgttcg gaaatctgaa tccaatgttg ttgcacacag tcggctttag
    19681 ggccggcttt tttttattcg ggaggagaac gatgggtacg ttttatggcc gatcactgtg
    19741 cgtgatcgac gatctttcac aagaagaacg gctgtacctc tttgagcacg cgcggattct
    19801 caaatcagcg ttcgagactc aagatgaagc cgtgctgaac cgatatcgaa tcgatgatcc
    19861 ggacttcgga atctatgagg tgtttctgga agattcgacg aggacgaagg aatcctttcg
    19921 caacgctgcg aagttccatc acgcaaaggt ctcggatctc aacagcggtt cctcctcctt
    19981 caacaaaggg gaaagttacg cggacacgtt ctataccctc agcggttact ccaatacgat
    20041 tttcatcgtc cggagcaaag tcgagggagt atgcacctat ctggaggaga gcctcacctc
    20101 ctacgccaga cgaaacggct tgaagcgaaa accggcgttc atcaacgccg gtgatggcaa
    20161 acacgagcac cccacccaag agctgcttga tgagttcacg ttcctggagg ataacaactg
    20221 gtcgacggaa acccttcacc tcgccctggt cggagatctg tatcacgggc gaacggtcca
    20281 ctccaaagcg gacggcttga agatctttaa gaacgtcact gtagacctga ttgcaccgcc
    20341 cgagctggcg atgcctgaaa catacctcca caagatgaaa gaccacgggt tcaccgtgcg
    20401 aaccttcgac tcgatcgatg cctacctcaa agaaacggca atcgccccaa agtggtattt
    20461 tacccgtcct cagctggaac ggatgggtga caaggtcctg aagaaggaag cggagctgag
    20521 gcgggcgatc accttccggg aagagctgat tccccttctg cctctgaata cgaagttcta
    20581 tcatccgctt ccccgccata aggcgcaccc gacgattccc acctttctgg atgaaacgcc
    20641 gctgaacgga tgggagcggc agagcatcaa cgggatgtat gtccgaatcg ttctccttgc
    20701 cctgatcgca ggaaagatcg ggggtgagtt tgtctcggca aggcctccgg tcgtcgaaga
    20761 ggaggattac atcaaggaag tgaacctcga ggaccgagtc ccccaagaga agcggatcag
    20821 cgaaggagtg cagccgattc acgatggaat cgttatcgac cacatcctcc gcgggtatga
    20881 gatcggtgag attcgcgacc atatgcgcct gatcagcagc gtattggaac tggatcgagg
    20941 aaagggaggg gagtgggttt ccagcggaca gcaagggatc cataaaggga tcatcttccg
    21001 ccccggctcc tatgagctcg atcgcaagca cctcaagcgc ttggcggccg tcgccccggg
    21061 ctccacgttg aatctcatca aagacggaaa ggttcagcgc aagttccgcc tctcgttgcc
    21121 gccgagaatc tacaactttg aagatctcgg gtgcacgaac gaggcgtgca tcagccatcc
    21181 cagccaggag gaaggggtta tggcccgctt ttatcgaacg caggaaaaca cctttgcctg
    21241 cgcctactgc ggcaaagagc atgggttcaa agaaatatgg aagcgcagca ggaaatagga
    21301 tagagttaag aagctatgaa caacctcaaa aagattactc aagcatatgg gccgaaaatc
    21361 gcccgggcgg cgttcgagct cggggcgatt cgcctcaacg tcaccgatcc cttccggtgg
    21421 gtcagcggct accggatgcc gatctacaac gacaaccgac gcctgctcgc ttccggcaaa
    21481 gtccgggcgc tcatcgccga ggggtttgaa gcgattctga acgcgttgga gtacgatccc
    21541 gattcgatcg ccggaacagc gacggcggga atcccccacg caaccacctt ggccgatcga
    21601 ttgggaaaac ccctttcgta tgtccgttcc tcaagcaaag ggcacggcct cggaaaccgg
    21661 atcgaggggc tggcgaaggg cggaacgtat gagaacaccc atgtcctgtt gatcgaagat
    21721 ctgatctcca ccggtctttc atcgacgagc gccgtcgaag cgatcgtcgc ggccaagggg
    21781 cagtgcccct atacgctggc gatcttcacc tacgggtttg cggcagccga agaagcgttc
    21841 gccgcattgg acccgaaagc ggcatttcat acgattctca cctatgaagt gatgctctca
    21901 agcgccctga aagaagggta tatctcacga tccgaggcgg aaagcctcga ggcatggagc
    21961 agcgatccct tcggatgggg aatagagcgg ggcttcatga gggaggaagg atgacgtaca
    22021 gggaattgct tgaaacaagt gcaaagcagg tcggaaacgt ggtgtgtatg ggcttggatg
    22081 tgaagccgga gcttcttccc catgctgaac agggaatccg ccaccggctg aacacctact
    22141 tccaacagct ctttcggcgg atgaatcttt cgggcctcct gccggctgcg ttcaagccga
    22201 atatcggcta ctatgctgtg ctggacaatc cccgggagga ggagttctca ggttcgctcg
    22261 ccctggccga tctcttcgat ctcttggaaa acttctttcc caatattcca atcatcctcg
    22321 attcaaagcg tggtgacatt gcgacaagca gtcggaacta cgccgatgaa gcgttcaatc
    22381 ggtggagagc gaacgcgatc accgtcgccc cctatatggg cagcgactcc gtcggaccgt
    22441 tcattcagga ttccacgttt ggaacataca ttctcaatcg aacgagcaat cccgggggga
    22501 aggatctgca gaacctcatc ctgcacagcg ggattccgct ctatgaagcg gtggccaagc
    22561 agatcatcgc ctacaatgcc gggaaggggc atgtcggagc ggtggtcggg gcaaccaatc
    22621 cggaggagct gaaagcaatc gccaaaacat atgccgagca gtcgattccg ctgttgattc
    22681 cgggagtggg aagccaggga ggctccgcaa ccgatgtgat gaggaccctc ggcgaggtga
    22741 actatccctt gccattggtt cggatcaaca gctccagcgc cttgacccat ccgtggaaaa
    22801 agggcccggc gccggatgac tggctcgatc aatgcgaggg ttctctgcga actctcatca
    22861 aggagtgctc gctatgagtg atgtgcttca acgcttgatg aatcaagagg ttcttcttgc
    22921 caccgtcagc ggggtgggtt ccacaaaagc ggaactgatc cgctacttcg atgagtcggt
    22981 ggaggcgatc gatctcatca ccaccaagag cttccagaac gaaccgaacc cggggaaccg
    23041 cgaaccgatc atctgtgaag tggagaaagg aacgttcgga aactcggtag ggctgagaaa
    23101 tcccgggctg gaggtggccc tccatgaact tcgagagctc aggaaaaccc actccttccg
    23161 agctctgttg aacgtgtcgc tctccgcctc cacgcctgaa gacttcgtaa cactcgtcaa
    23221 agcgtttgat gaagtcgccg atcttgtgga actgaacttc tcctgcccgc acgccgagcc
    23281 ggggtacggc tcgacgatcg gggagaacct taagcttgcc gcccattacg tgcgcttcat
    23341 caaggcgaac accgtcgaat gcgaggcgcc cctgtttgtg aaattgacgc cgaacgtcga
    23401 agatatcgcc tcgatcgcca aagcggtcgt cgaagcgggg gcggacggga tcgtcgcgat
    23461 caatacggtc ggaccgctcc tctacatcga tcctgcatcc agaaagccga ttctccagaa
    23521 caggctcggc ggaaagggcg ggcaaagcgg aaaagcggtc tttccgatcg ccctcaaggc
    23581 gataaaagag attcgggaag cgctcggaac ggggatcccg atcatcggaa tgggcggggt
    23641 cgattcaggg gcggatgcgg cgaagctcat cgaagcgggg gcgaacgtcg tcggcatcgg
    23701 atccgctctc ggcacggtgg accaacgctc ctggcctcag tatctcaggg cgatcaaaga
    23761 ggaggcggat catctcctct atagcggaaa aaagcctgcg gtctcttccg cttccttcat
    23821 caagaccgat cgccagatgg agtatcgaaa gcacatcgtg cttgagaatg aaccctacgg
    23881 cagtgacacg gcgattcttc ggctcgacgg caggcttccc accaaagccg ggcaattcgc
    23941 ctttttatgg ctgccgcaga tcggggaaaa acccttctcg gtagcccaca acgatccgct
    24001 gaccttcgtg atcaagcgaa gggggccgtt cagtgatgca ctatgctcct tgcggaaggg
    24061 agacgaagtg tatatccgcg gtctctatgg ccaaggcttc gatcctccgg aaacgaagaa
    24121 agccctgctc atcggaggcg gcacgggcgt ggcggtgctg cccgccctag ccgagcagtt
    24181 gtccgaacgg ggaacagagg tcgagatcct ggtcggaacc agcgaagcgg ttgaaacgac
    24241 cgccctgctg gaagagaagc tttcctccta cggatcattc gtctgcgtag cggatgaagg
    24301 caaaccgggg cgggtgctgg atctgctcga cgggcgaacg tttgatgggg agacggcgtg
    24361 ttatctggtc ggcccccaag tcttcatggc gcttgccgcc cgggcggtac tcagtggggg
    24421 agtgaaggaa gagatgctct atctttcgca ggagctgatg acattatgcg gaatcgggat
    24481 gtgcggggag tgcgtctgtg gcgatcggct gacgtgccgg tggggaacgt tcatggagta
    24541 ccggtatctg aaagagcatg ctccctccct gatcgcctat gattgatccg cacgtccacc
    24601 ttcgagattg gaacctcagc gcaaaggaaa cgcttgccca cgggcttctc gtggctgaaa
    24661 gaagcgggtt caccgcagtc atcgatatgc ccaacaccga tccgccgttg acgacgaaag
    24721 cgcggatcat caagcggatc gaagccgcaa acaggacaaa gaggaacgtt tcctatcatc
    24781 tctgggcagg ggtgacaggc gaccccgctc agatcgacga ggttgtcaaa agctgcgaag
    24841 agctctttcc ttccgtagtc ggattaaagc tcttcgcagg acactccacc ggcaacatgg
    24901 gccttgtcga agaggagatc caagggtggg tctaccgcca tctggcgagg cgaaactaca
    24961 ccggtgccgt tgtgctccac tgtgaaaagg aagcgctgct ccgccccgag ctgtatgaca
    25021 atgccgatct ttcaacccaa tccctggccc ggcctcccga ggcggaggtc gcttcgatcc
    25081 gggatcagct ctccttcagc aaggaggcgg gcttcgccgg tcacctgcac atcgcccacc
    25141 tgtccacgat cgaagggctt ttgcttgtgg aagaagccaa gagggaggga aggagaatca
    25201 gctgcggcat aacaccgcac catcttctgt tgaacgagga cacggcgaaa gatcggaggc
    25261 tgtatgcgaa gatgaatccg ccgctccgcg atgaggagac tcgagaagag ctctttgaag
    25321 ccctgcttga agggcggatc gactgggttg agagcgatca tgccccccat acgatcgccg
    25381 ataaggaagc gggggcaagc ggcattcccg ggttcagcgg aatgctttcc ctgttgaaag
    25441 tgctgtatga gcacaatctc tcagactcgg tgctgaaaga cctctttggg ggcaatgttc
    25501 aaagaacgct gaaccttcct ccccgagaaa tcgtgctgcc ccggtatgag acgctttccg
    25561 ctctgatcgc ggagacctcc ggggaatatc cctacgacag cttcagaggc ctatttcaga
    25621 gctgatctcc gcaagagctt cgatcgtccg ggcgatgatg tcattgattt caagaccgag
    25681 cagctccgct ccttttgcga tgacttcacg gttcaccccc ttggcgaagg aggggctgga
    25741 ccattttttt ctgacgctct tcacactcat ccctttcatc cgctccggcc tcatcagggc
    25801 gttcgcatag atcaacccgg tgagctcatc aatcgtgtag agcacctttt ccatgaaatg
    25861 gaccggttca acatcactgc aaagacccca tccatggctt accaccgctc ggatgaacgc
    25921 ttcatcgacg tcgatctcgg agaggagctt cggagcgact tggcagtgtc ggtcggaata
    25981 gagggcataa tcgatgtcgt gaagaagtcc gaccaacgac cagtaggtct catcatatcc
    26041 ataatgccgg gcgaagcttt tcatcgtcgc ttccacgcaa taggcgtggt ggagatgagc
    26101 ctcatcgggg gtgtattttt tcaaaagagc aagcgcttca cttcgggtga ccatggtgtc
    26161 ctccttttgg cgaagtatag taggatatga aaaaagagaa aagagaaatc tccttgatat
    26221 actgccgcat ttggtgatat attcgctcag aatcatgaat atacccgcat gcaccggaac
    26281 ctatatcgaa gaagtaattg cttccctaac tgacctttac actgatgtcg agaatgcaac
    26341 ccatgcattt gcctcccgct atggcatcgc ctgcccgccg gggtgcggat cgtgctgcga
    26401 acacttcatt cccgatatca cccacagcga ggctgcctac atcgccgcct atctgctctt
    26461 tgaactcaaa gatgaagaaa tgatcgaaag agtttatcgg cacagcggag cgggatgccc
    26521 gctgtatcgg gcggatacgc ccttccattg ctcggtgtat ccggcacggc cgctcatctg
    26581 ccgattgtac gcatccagcg cctaccatga caaatacggc aaagcgctct ttcgatcctg
    26641 caaatatgag gaaaccaaca cgatgcccaa acatctttcc tttgacgaac gggtcgtaac
    26701 gatgcaggat tttgcctacc gcctacgctc ccttgatcgt gaccggggcc ggctttcgct
    26761 tctttccgag ttgctccccg agcttttgga gcagctccaa ttcatcgcag cgcttgttcc
    26821 tgctcaagat ggccctgaag agccattggc ggggtaggct gtccaaaagt taaaatatgc
    26881 atttgcaggg gtataaaagc atgcaattcc ccgagttccg cagccaaaag cgcgaaaaag
    26941 gcttctaact tattgcaccg ttggcaatta atctgaagct tccagccata atatccgctc
    27001 aattgtagta tatcgatacc tgtccttcct cgtgctttgt ttgttgacta tgagcgcacg
    27061 ggtcatcaat aatagccgta tggctggaaa gattaaagca atactgtttg atttggatgg
    27121 gacgctcatc gatacgatcg aagacatcgc tgcggcgttc agcgccgcaa tcgccaagga
    27181 cgggttggag ccggctacgg tagaggaaac aaaactcgta gtggggcggg gattggtaaa
    27241 cgcgttgaaa gggttgctgg agattcgtaa tcatcctgtg agcgaagaac gctttaccga
    27301 actgtttcac gttttgtaca cctattatga agaacatccg gtggtatatt caaaaccgtt
    27361 cgatgggatg attgaattgg tcagcggttt gcaggatact taccaactgg gagtcatctc
    27421 caacaaagac catgagctga ccctcaggat cgtatcggaa ttgatgggcg atgtcccgtg
    27481 gagcgtcatt cgggggatga acgacgaata tccccgaaaa cccgatgttg gtgcattcac
    27541 tccttttgtg aaaaagatgg atttggggct tgaagaaatg ttgctcatcg gtgacagtga
    27601 agtagattac caaagtgcga ggggggcgaa catggatgta atccttgttt catggggatt
    27661 cagaccgcgc gaagcccttg ttgctcttga aggggcttgt gttgttgatt cggttgaaga
    27721 gctgaaggag gcgatttatg cgttataatg aaaaggactt gagacaaaaa gcgactctct
    27781 accttgaagt agaaaccgaa gaagcgtttc gaactgaagt agagacggaa ttggaaaacg
    27841 gccagtgggc ggagctctat gagcgcttct ataccactct ttcttttgga accgccggaa
    27901 tacggggcct gcttggtggc gggaccaacc ggatgaatac attcatggtc cgaaaagtca
    27961 ctcaagggct tgctgactac ctgaaaaccc aatcggatca tccttcggtg gtgatcggct
    28021 atgacagtcg acactacagc gacgtgtttg ccaaagaggc cgcccttgtc cttgccgcga
    28081 acggcatctc ggtctatctc tttcccgagc tgaggccggt gcccatgatc agctacgcgg
    28141 tacggaatct caaaaccacc gccggaatcg cgatcaccgc cagccacaac ccctccgcct
    28201 acaacgggtt caaagcgtac tggagggatg gcgcgcaagt gacccctccg cacgatttcc
    28261 aaatcgccga tcgggccaat gcggtattgc cgaaacagat tcgaacgatt accgaagaaa
    28321 aagcccgctc caacggcttg cttgttcggg ttccggataa agtggaagaa agctactaca
    28381 atgaggttct ttcttccatt cgcaacatgg acatcatcaa aacgagcaaa accctcgttg
    28441 cgtatactcc cctgcacggc tcgggcctca agccggttac ccggctcttc gatgaggtgg
    28501 gaatcgatta tgtcaccgtc aaggaacagg aagaaccgga tggatcgttc ccgacggtga
    28561 agctgcccaa ccctgaagat catgatgcga tggcgttggt gttgcgcctt gcaaagaaga
    28621 agggggcgga tattgttctg ggtaccgatc cggatgcgga ccgcctcgga atcgccatcc
    28681 cgctgagtga tgaaaagaaa gaatatcagc tcttgacggg caatcagatc gcagtccttc
    28741 ttgccgacta tctgatgatg accaccgggc accggacgaa acaaccggtc gtggtgaagt
    28801 cgatcgtcac tacggatctg gtaaaagcga tcgtcgagaa gcggggcggg gtctgcaagg
    28861 atgtattgac cggattcaag tacatcgcca acgagatgac actcctcgaa ggcccgaaag
    28921 gggaggagca gtattttctc ttcggctgtg aggaaagcta cggttacttg acgatcaata
    28981 tcgtccggga caaggatgcg gtttccaccg ccttggtcgc cgttgagatg ctcagctact
    29041 ggaaagaccg gggcaagacg ttgatcgatc gcctcggaga gatctatcgg gagttcgggt
    29101 attacacgga gcgagtcatc tccgttgatt ttgaaggcgc cgccgggcaa gaaaagatgg
    29161 tcaaggtcat gcagtccttc cgcgacctga cggcgggtga ggagctggtc gggcgtaaga
    29221 tcttgcagat cgaggacttg atgaaccctc aagagggcgg gcttcctccg agcgatgtgg
    29281 tgattttcca tctggaaggc ggtgaacggg tgattgtacg ccccagcgga accgaaccga
    29341 agatcaagta ttatcttttc ttttccaccg aagcggtgaa ccgtgaagcg ttcgatgcac
    29401 accttgagag tcgagtcaag gagatgaggg agggactttt gtgatcgagt gctatacgtt
    29461 tgtccgatac aataaaccgg tattgacgtt gacccccttt ctccgggaag ggcacgacct
    29521 tcttggagag caggtggtga tgtatgccgg cggaatgctg aacgcccagc aaaaagatca
    29581 ggcgaccttc tccctcttca gccagatcga ctttgccgtt gaccggtgga ttcaggacaa
    29641 acggtatgtt ccccgtctct tattcagtgc gctcgccttc atggtgagct atctcttctt
    29701 ctcgctggtt gtgcgcgatc cgcttccgat gatcgatgag ctcctcatct ccagtgggtg
    29761 ggcgatcgtc gtatggatta ccctctcccg tcgagatacc cgctcgatct tggctcagga
    29821 gaaccgtcag cgtttgaaga tgatcggggg gaagcggagt gagcaaatcc aagagaacct
    29881 cttttcgatt gaagagtatc ttgatgtctg tgccaaagcc gataccaagg agcttgccaa
    29941 gcagcttgtc gagggaacgc tcccccgctg gacacacacc atcgaggcaa gcgagcgaag
    30001 cctccttcgc acgctgctcg atcggtatct tgcggtgtat gaaaagccga ccgcccgttg
    30061 gattcagcgg ctggatcggt cgagggggaa agaccttgct acgaaactct atcttcaagg
    30121 ttccgagggt tcggtggatc tcagtctgct cgccctgagg tgcgctctga agcaaagcga
    30181 ggaatgacgc cccttagggg gcatgggcct tcctgttctg tgcatcctct tttgctatct
    30241 ttcgtattcg ctcagccccc tctttgatta ccggtacctt gcaggggggc tgtttttggg
    30301 ttcattgggc ctttttctca gatggggctc ttggagaaga tacgccctgt gctcctattc
    30361 gctcatcatc ttcctgctct acctgctctt tggccgggcg gttcatctca cccgtttcac
    30421 ctgcggattg gaaacgaaga gaatcaccgc tcttgaaggt gttctcttgg aagattcgct
    30481 cctgaccaag agcgcgaacc aactgctgcg catcaaggtg gcgtggtgtg aagcagcgga
    30541 tggatcgggc gcttcggcat ccggggtgtg cagcgctctc ggttcaagtg atgagattct
    30601 gtttgcctcc tcgacgatcc gggtggtcgg cgccttccat gaggaatctt cccttttcat
    30661 cgccgaggat cttcagatcc gtgacatccc ttcatttgct cgatggcggc gttccgctct
    30721 ggaggggatt cgacggaggc tgaagaaatc agtctccgat gaaaaagccc gatcccttgc
    30781 tttcatgctt ctcctcggtc aaagcgacag cagcgccttt cccctcaagg agctggctcg
    30841 gagaaccggt ctttcccatc tgctcgccct ttcagggatg catctttcag tgtttctggg
    30901 tctcagcacg gctctttgtt ccctgtttct cggttcctac cgggcgaagc gggcggggat
    30961 ggtccttccc tgtctgtttg tgctcatcgc cggtcccaaa ccttccttgg tccgagctct
    31021 tgggttggct ctctgctccc tgattccttt cggctctcat ggcagacgct attcctacct
    31081 gcttgctctt tccttgcagc ttctttggtt tccgtacagc gtgggatcgc tggcgtttct
    31141 cttcagctgg gtggcgtata cgatgatata tatcgccccg atgcttcccg cgtttccgtg
    31201 gaaaaccgga acgttggcgg tattggcgat cgcccccgcc acgcttctcc ttgagggtag
    31261 ctggaatgcc ggaggaatat tcctgacgcc ggtcgccgga ttgctgatca acctgtcgat
    31321 gatctcctcc ctccttgtcc tgatcggcgg ttcgtccttc gcccgagtgc ttcttcggtg
    31381 tgatggattg ctcaatgacc tgcttctttg gggcgcctca tcaaacctcg tttttggctg
    31441 gaaagggtac gcaatcgcgc tcgctttcgt gttgaccctc ttctttgctc tcatctatgc
    31501 taaaaggaac ctattgaagc aggagaccta tgagctggac ctttccctac gattcaccca
    31561 acgcgatcac cgcccttctg gaggaggagg ggctggcgat gagcaagaag tttggacaaa
    31621 acttcctcat ctcgaactcg agccggagca ggatcgtcga tctgctcgat gtgcaggggg
    31681 gatcgaaggt ctgggaggtg ggaccgggaa tcggtgcgat caccgccctt cttcttgaac
    31741 ggggggtccg cctgaccgcc tttgaactgg atcacgggtt ttgccggatt cttcgcaccc
    31801 gagcgttcgg tgatgaacct cattttacgc tcatcgaagg ggatgccttg aagacgcttt
    31861 ttacagccct gagagaaaag ggcacccccg agcgtctctg cggaaacctt ccctataata
    31921 tcggctcaat tctggttgcc cggcttcttg aaggagaaag ccggcccaac cggatggtct
    31981 tcaccgttca gaaggaagtg gcggagcgga tatgcgccgg tgaaggatcg aagaattggt
    32041 cctccttctc actgcttgcc caggcggact atgatgtggg gatcggattc gatatttccc
    32101 ccggttcctt ctatcccccg ccgaaggtca cctcctcggt ggtcgtgttt cagcttcgat
    32161 cagtgagtcg gattccacag gcggatcgcc atgcattcta cctcctcgtg cgcgaacttt
    32221 tcgggcagcg gcggaaaacg cttcggaaca atctgcttca atcgaaggtg ggggctcgaa
    32281 tcggcaggga gggggttgat tggcttctcc ttcaaagcgg gatagagcct tcaaggcggc
    32341 ccgagacgct cctctgggcg gagatcctct ccctcagtgc acatctgagc tcgattccag
    32401 ccacccgtag cgaaggaaca tcgcaaggcc gatgattccc ctcagcagga ggtcggaggc
    32461 catgccgatc cagattccca ccaggccgag ggaggtgcag cgaaccatga agagcgccag
    32521 aggcagcctg accacccaca tcccggcgat cgagatatag aagggacggc gggtgtcccc
    32581 cgaaccgcgg aacactccga agacgagcat cgagagaccg aacgccggtt cgacgaacgc
    32641 ttcgatccgc aggatcgtgg ttcccaaggc gatgacgttc cgatctcggg tgaagaatcc
    32701 catcaggtag ggagcggtga aatagagcac cacgcccatc gtggtcatca ggagcgttcc
    32761 gatcagggtc gtcgcgtttc cgaagcgctt tgccaatgtc ttgtctcccc ggccgagcga
    32821 ctgggcgacg agggtcgtcg ccgcggtgga gactccgctt gcgggcagat aggtcatcgc
    32881 ctctgcggtg tttgccaaaa agtgggccgc cagcgcggtc gtgcccagcg acccgacgat
    32941 tttggtcaga acgatctgtc cgaaggagag ggtggagcgc tcaagcccca cgggaagccc
    33001 gaggcgaaac gcattttttt gaatttccct gttcagagcg aatgattgat tcagctccag
    33061 ctgaatgatc gaaggccgag tcagccagat gatgaagagg agaatcgccg taatcgtcac
    33121 cgccaagctt gtcgcgatcg cagctccttt cacccccaga cccagcccat gaatcgtgat
    33181 cgggccgaga tccacttcac cgaaaatgaa gaacatattg aggataatgt tcagtatatt
    33241 gttgagggtg ttcagaagaa gcggggttcg ggtatcgccg ctgaggcgca tcagggaact
    33301 gatcgtgatc atcaggaggt tgggaaggta cgccaaggcg atgacggtgt aatagccgcg
    33361 ggcgagggtt tggacgtgtt cttccgctcc gatccacttt ggaagcactt gggcgatgag
    33421 ggtgaagacg gtcgagagga tagctccgaa gatcagggct gtcaccagcg cttgacgagt
    33481 gatcattctt gcccgctcaa atcggcgccc tccgatgttg cgcgccatca agaccgcgta
    33541 cccgatcgca aacgcattca tccatccgtt gacgagccag atcgtcgagg tggggacgct
    33601 gactgcggcg gtggccgctg ctcccagaga gccgaccatc gccgagtcga catacgtgac
    33661 ggaaacctga aggatctgct ccagaatcgc cggccatgca agggtccaaa tgatcaggcc
    33721 gatcgatttt tcctgatcga gcagtccctc ggtcatatgc tcctacttgg cggcctgggc
    33781 gagggtgatg gcgctggtga cgacgatgtc ctcaacggaa cagcccctgg aaaggtcgct
    33841 gatcggtttg gcgaatccct gaaggatcgg gccgtacgcc tccgcgcccg caaggcgctg
    33901 aacaagcttg tagccgatat tgcccgcctg aagatcgggg aagatcaggg tgttcgcctt
    33961 tcctgcgacc ttggagttcg gcgctttgga tgcggccacg gaaggaacga tcgcagcatc
    34021 cagctgcagc tcgccttcga tcgccaagga ggggttcttt tcccgaacga tcttcgtcgc
    34081 ttcgaccact ttttcgacga gctcaccgct tgccgagccg agggtggagt agctgagcat
    34141 gccgactttc ggttccacgc cgaggaaggt ggaacagctg gctgccgaag cgagggcgat
    34201 ctcggcaagc tgttcggctg tcgggttggg aatcgttgca cagtcggcaa agatgaacga
    34261 gccgtcggct ccatactcct tcttatcggt agccatcacg aagcacgaag aagcaaaact
    34321 gatgccgggg gcggttttga tgatgttgaa cgcggccaca agtaccgctg cggtggtgtt
    34381 ctccgctccg gcgaccatcg catcaccggc tccgagacga accatcatcg ctccgaaccg
    34441 aagaggattg gcgatgtctt caaaagcctg ctcttcggtc atccccttgt gcttgcgcag
    34501 ttcatagaac tccgtcgcgt attgcttgcg ccgatcgctc gttgcgggat cgacgagtgt
    34561 cagcccttcg agcgaggtgg aggatgcgct tgccgctttt tcgatctccg ccttggatcc
    34621 gatgaggatc acctcgctgg ccaagtgctc gtcagcgatg atccgagcgg ctttgatcgt
    34681 tcgctcttca cttccttccg ccaggacaag ccgtttttga gcagcttttg cctgagcctt
    34741 catcttctct gcaaataaca taattcatgc tcctaaaatg tggtatcgat gctcaatcag
    34801 tgtaccacgt ccggcaatct tggagaagac tcgtgaattc tgtacaatac ccttcatgag
    34861 tgaaaagaat cgggttggag tatatggtct tgggcgcttc gggctctttt gggcgacccg
    34921 gcttgccgag aaggggtttg aggtcgtcgg ctgcagccgg cggaccgtgg agctgccgcc
    34981 gaatgtccgc caagtgagcg tcgaagaggt gttggattct ccgaccgttt tttattgtgt
    35041 ctcgatcagc gcgttcgaag aggttcttgc caacagcttg ccctatctga aggaaggggc
    35101 ggtcgtcatg gacacctgtt cggtaaagct gtatcccgcc cgcgtgatga agcgcctcct
    35161 ccctccgacg attcaggcga tcgcgaccca cccgatgttc ggtccggatt cgggaaagga
    35221 cggactggaa ggactgccct tcgtcatgga caatgtcagt gctcacgagg agacctggcg
    35281 atggtgggtg gatgagttca cctcgtggaa gatggatgtg cttatgatga gctgtgacca
    35341 gcacgaccgt gaagcggcgt ggagccaggg aatcacccac ttcatcggcc gaacgcttgc
    35401 cgagctgaat ctgggatcga cccgcttggc gaccaaaggg taccagaccc tcatgggggt
    35461 ggtggagcag acgtgcaacg accccttgca gctcttttat gatctgcagc ggtataatcc
    35521 ctacacgaag cagatgcgga tgggcctgcg gggagcgctg gtgacgacga tggaggcgct
    35581 gaaggagcag gaggagatcc tttgagggtc tgcgtctata ccctgggatg ccggctgaat
    35641 cagtgtgaaa gcgaagcgat cgccgactcc ttcgcccgcg aagggtttga aatcgtcggt
    35701 gaacaggacg gcgccgatct ctatatcgtc aacacctgca ctgtcacctc gaaagcggag
    35761 cagaaagccc ggcggatgat ccgccgattc gctcaaagct caccgacatt ggtcaccggc
    35821 tgctatgccc agctgaaccg agaggagctt gagagcctcg gcaaagaggt gctggtcgtt
    35881 ccgctggacg ggaaggcccg actcctgaaa cttgccgcgc atcttcaagt ggcgatggag
    35941 agttcattct ccctcttcga agggatgaag agtttcaccg gtgaagacgc gacggtcttc
    36001 gattacgctg ccgccacctt ttcctaccac agccgggcgt acctgaagat tcaggacggg
    36061 tgcgacaaca gttgcgcata ctgccgggtc catgtcgccc ggggcaaggc gcaaagcctc
    36121 gatcgggcca ttgtcatcga gcgggcgaaa gcccttgaag aggcgggctt caacgagatc
    36181 atgctcaccg gggtgaacct cacgatgtac gatcatcgaa acgaggggct cggcgggctg
    36241 ctggaagcgc tcctgggaga aataggagat cttcggatcc ggctctcctc gatggagccg
    36301 gatcatatcg acgaacgcct gctcggggca ttggaagatc cccggatgca gccgcacttt
    36361 cacattcccg tgcaaagcgg atcggattcc gtcctgaagc gggtaggcag gaagtacacg
    36421 atcgccgagc tgacggcgat cctcgaccgg ttgaaaaccg tcaaggacga cccctttctg
    36481 gctgcggacg tgatcaccgg ccttccgggc gaaggggagc gggagttcga ggaaacctac
    36541 atgtttctta aagaaatcgg cttcagcgcc ctccatgtct ttcccttctc tccccggccc
    36601 gatacgccgc tcttcaacgc gaaggaccgg gtgccggagt cggtccggga tgaacgggcg
    36661 aaaattctcc gaacattgag cgccgagctc accgatgcct acctgcgccg gcaggagggg
    36721 aaacaaggcg aggtcatcgt ccaggggcgg aaaggcggct tctggtgcgg ggtgacgggc
    36781 aactacgtgg atgtaagaat cgtgaatccg ccgctctttg cccagcgggg aatgctcctt
    36841 gagggcgtgt tcggtaagaa gaccgatcgc ttcgtggagt tcatcgtcac tgagacttcc
    36901 tgatctcccg cttgcggatc tccggctcgc cgatcgtcac caaggtattg ggagcgaccg
    36961 attccttgat ccacacgttc gatccgatcg tcgttccccg tccgatcgtg atgtccccga
    37021 gaaccgtggc gttggcgtag atcgttacat cgtcttcaat cgtcgggtgg cgcttcgccc
    37081 ccttgatcag agccccgcac gcatcacggg gaaaggagag cgctccgagg gtcaccccct
    37141 gatagatctt cacgttgctt ccgatgaccg tcgtctcgcc gatgaccgtt cccgtgccgt
    37201 ggtcgatgaa gaaggaggaa ccgatttggg ctccggggtg gatgtcgatt cccgtttgcg
    37261 catgggcgta ctcgcccatc atccgcggaa tgatcgggac gtcgaggcgg tagagctcat
    37321 gggcgacccg gtggatcgtc aggcttttga tgcacgggta ggagaggatg acctcccgga
    37381 tgtttcgggc ggccgggtcg ccttcgtagg cggcctgagc gtcggctttc aggctgagcc
    37441 gaatcgaggg gaagcgggaa gcgatgcttc gaaccgcccg gtcgcttctt tcctccgccg
    37501 cttcagagcc gagcgtttca tcatcgtagc ggaaggcgag gtagagctgg cctttgaggt
    37561 ggctggtgac cacatccatc tggtaggcca gatactcctc caaatccatt ctggcctgct
    37621 cccttccgca ccgtcccggg aagaagagtt ccagcaattc gtcgcagagc tggccgacga
    37681 tttccattcg cggcagcggc ttcagaccgt gaaagttcgc cttcccggcg atccggtcga
    37741 aggtcttcag aaatgaaggg atgaatacgg ttgagtcaaa cggatgatct ttgcatggtt
    37801 ccatgaccaa cagcttacgg gttctcgctc cctttaggca agaagggagc gcacccgttc
    37861 tgaaaaacgc cgagcgaggg cgatcgccaa tcccagcttg atcccgtccc cgaccaggaa
    37921 gggaagcatt cccactgcaa ttgcctgaga aagggagatc gagagaagat gcttgagcca
    37981 cgatactccc ccgagataga ttacgattgt cgccaatatg ccaaagagga ccatcctcca
    38041 aatccgctta aagcccccga agccggcgat cagggctgcg ggaagcagcg caaggaggta
    38101 acctccggtc ggcccgaaca gcagggcgaa gccgcctccg ccgctgaaga ccggcaggcc
    38161 gatcgcaccg aggaacagat agatgccgac gctcatgagg gcgacctggg gccctcccat
    38221 ataccctgca agcagcacga agagagtctg cagggtgatc ggaaccggcg gaagcggaat
    38281 ccggataaag gatccgacga tgatgagggc ggtgaaaaag gcgacaagga ggattcggcg
    38341 ttcgttcatg gcgggacgat agcacaaaga ccggcaggtt gtaaaccata acaaggttga
    38401 caataaaaca atcacttttt atgctcatcg tatgagcaga aaaacagagg tgcttgcccg
    38461 tctccaacaa gctttgaatc cgctctcggg ggagaggatc gcctccgatc tgtcaatctc
    38521 ccgagctgcg gtgtggaaag cgattgaggg tctgagaaag gatggatatc tcatcgaggg
    38581 tgttcccaat caggggtatc ggctttcttc ggttcaaccg ctgcttaacc aatccaagct
    38641 cttctccctc ctgccttcct atcggatcga aggagtcgac gcgatcgact cgaccaacag
    38701 cgagctgaag cggcgaaacc ccaaaacgaa aactctcctc ttcgccaaca cgcaaagcgg
    38761 cggacgggga aggctcggca ggtcgttctt ctctccgcgg ggaggggtgt atttcagtct
    38821 ggctcttccc ttgaacgtga gcatcgattc cgcattgctc gtcacctcga tcgctgcggt
    38881 ggccacggcg gaggcattga gcgaaattgc tcatgtcgag acctcgatca aatgggtgaa
    38941 cgatctgtat ctgggggaca aaaaactctg tggcatcctg accgaaggga tcatctcgat
    39001 ggaaacgaag acgcttgcga cggtgattat cggcatcggg ataaaccttc atctgccgat
    39061 cgaccgattc cccgccgagc tgcaggcgat cgctaccagc gcctttgaca gcgaacggcc
    39121 gctctcgatc gacccgaacc gtcttgtgca aggaatcgtc accaacattg aacgattgat
    39181 cgaagacctg cccgatcgtt ccttcctgga gatctaccga agaagaagca acctcatcgg
    39241 aaaaaccgtt caggtccatg aaggcgttca ttcccatccg gccagggttc ttggaatcga
    39301 tgatgacgcc catctgctga tcgccgatga aacgggaagg acccgcagcc tttcaagcgg
    39361 agaagtcagc attcgccttg aagggtaggg agcctttatt gtaggatgct tccagaagga
    39421 agatacgatg ctcagagcaa tacgcgcggg ttggaaagcc cgcattgaag aagaattata
    39481 caagattgct gaaacgaacg ggaaggtgag ccttcccgaa ctgatcgttc agacgccccc
    39541 caaggttgaa ctcggggata tcgcgtttcc ccttttcgcc caggcgaagg tcttggggat
    39601 gagtccgccg gcgctggcaa atgaactgaa gaaccgcttg gatgcccaag aactcctcgt
    39661 ttccggtccg tacctcaacg tccgaatcga catgaacgcc cttgccctcg atctgacgaa
    39721 ggcgatcagg aaagaaggtg atacctacgg gcacaacaga acgctcggtg gaaagcgaat
    39781 agtcgtcgag ttctcctgtc ccaacacgaa caaaccgctc cacctcgggc atctgcgcaa
    39841 cgactcgatc ggcgagtcga tctcggcgat tctcaaggcg aacggagcgg aggttcggaa
    39901 ggtcaatttg atcaacaacc gcggggtgca catctgcaag tcgatgcttg cctatcagaa
    39961 gttcggcaac ggggagaccc ccgagtccgc cgggaaaaag ggcgatcact tcgtcggaga
    40021 ctactacgtc ctctacaacc ggaaggccga agaagatcct tcgttggagg ggcaggctca
    40081 ggcgatgctc aaggcatggg aggacgggga tgaagaggtc gttgcgcttt gggagaagat
    40141 gaaccgatgg acgctggaag gcctttccca aagctatgag cggatgaggg tgagcttcga
    40201 ccactactac tatgaatcgg aaacttatcg ctacggcagg gatgaggtcc tcaaaggggt
    40261 cgagcagggg gtcttctatc gggaagccga caactcggtg tgggtggact tggaagcgat
    40321 cggtctggat aagaaggtgc tgctacgcag cgacggcacg tcattgtata tgactcagga
    40381 tttggggacg gcgatccggc gatatgagga ttggttcttc gactcgatga tctatgtcgt
    40441 cgccagcgag cagcagtacc atttcaaagt cctcttccat gccctcggcc tgcttggcta
    40501 tccgtgggcg aaggatttgc accatctctc ctacgggatg gtgaacctgc ccgacgggaa
    40561 gatgaagagc cgggaaggga aggtggtcga cggggatgat ttggttgaag agcttaccct
    40621 cctggcaaag gaggagatta tcgccaagga acgggaggag cttgtcggcg atcttgaaga
    40681 aacgagtctc tcgatcgccc tcggagcctt gaactactat ctgctccaag tctctcccac
    40741 gaaggacatg atcttcaatc ccgccgaatc gatctccttc aacgggaata ccggtcccta
    40801 tctccaatac atgggggctc ggatcagcag catgcttgcc aagtatgagc aggagattcc
    40861 cgcctccttc gacgcgagcg tcctcacctt ggaggatgag cgctccctga tccgccagct
    40921 tgcgctcttc ggcgaggtgg tcgagcgcgc cgccgagggg tatgatccct cccagatcac
    40981 cgcctatctc tacgaactga gtaggacgtt cagccggtgg taccatgaca atccggtgct
    41041 caaggcatcc acgcccgccc ttgtcgaagc gcgggtggtt ctctcttcaa atgtccttca
    41101 ggtcctgaaa aacgcgtttg ctttggtcgg aatccccttc ctcgctgcta tgtaggggca
    41161 tagtgggtac agaaacgacg aatgaatcgc tcggcttggt agcgaatctc ttcatcggta
    41221 aggagcgttc gaaccagatg tcgagcgatt gttgcactga atgtttcaat cgccaattca
    41281 agatcgtcga tccggagctt tccccgctct tcaagatgtt cgatcagcac ggagctttgg
    41341 gcgatgagca tctcatcgaa ggtctgcttg atcgtccgtg cctgcccgtt tgtccatgat
    41401 tcctgttcaa cgatccggta aaaggagtag agcggctcgg tttcatagag ctcgctccag
    41461 tggtcgagag ctcgaagaag cacctcttcg gcgtttccct ccagatcgat agtcctcatc
    41521 cgtttcagcg ccaggcgatg gcaataggcg aatacctctt cgatcagatg ggctcggccg
    41581 gtgaagtggt ggaagatcgt cgctttgctg attccatttt catttgccag ccgactcaag
    41641 ctgacattgc caagtccctg ctcactggcc agagaaagca tgctgagaat caattgttcc
    41701 ttcacaggcg catattaacc gaccgttcgg tagggagtca agaggctttt ctttggaaat
    41761 acaagagtta tttagaaaat cacttgcatt tttacttgta attatggtac ataaaggttc
    41821 gacaatcact ggtaaggaag aaagcgaaat gtacgcactt gtagaaattc tcggaaagca
    41881 gtacaaagcg atcgaaggcg agacggtcca agttgattat atcagcaagc aggaagtcgg
    41941 cagcgccatt gagtacgcga cggtacttgc agtcgttgat gagaagaatg cccgctttgg
    42001 aactccgtat gttgagaacg ccaaggtcaa agtgaccctc gttgaaaaca ttaaggggga
    42061 taaaatcagg gtgttcaaga agaagcgccg gaagggctat cgcaagaccc agggacaccg
    42121 tcaatggtat tccctcgtga cgatcgacaa gatcattgca taaggaggac tgaagatggc
    42181 atcaaagaaa ggtggcggtt caacccgcaa cggacgcgac agcaaagcga agcggcttgg
    42241 tgtaaagcgc tatggcggcc aattggtcaa agccggtgaa gtcctcgtac ggcaaagggg
    42301 aacgaagttc caccccggcg agaacgtcgg cttaggctct gactatacct tgtttgcgaa
    42361 ggaggcggga catgtcctct tccacagcag acgaaacaga aaatatgtga gcatcgtaac
    42421 ggagtaaggt tcattcgagg acctctggat gattggattt tctgacgaaa cgtatatcga
    42481 cgttgcctcc ggagacggag gcaacggtgc tgtatccttc cgccgtgaaa agtttgttcc
    42541 ccggggcgga ccggacgggg gcgacggagg gcggggcggc gatgtggtat tcgttgtctc
    42601 gcacaacctc agaacgctcg cccacctcaa gcggcagcgc tccttccggg cggaacgggg
    42661 acagaacggt tcatccgccc gcaagtacgg tcgggacgga gtggatgtag tcattcccgt
    42721 tcccccggga acggttctca aggatcccga aagcggtgag attctcgtgg acctgacgga
    42781 ccatgagcgc tacgtttttc tcaaaggcgg acgaggcgga cagggtaact ggcacttccg
    42841 ttcgtccacc aatcaggctc cccgctatgc ccggccggga cagaaggggg tggctctccg
    42901 aatcggggtt gagctcctga tcatcgccga catcggcttt gtcggctttc cgaacgcggg
    42961 aaagtccagt ctcctcaaca cactcaccaa tgcccgcagc gctgtcgccg attatccctt
    43021 cacgacgaaa gttccccatc tggggatgat gcgccaccat gagcgcgata tcgtgctggc
    43081 ggatattccc ggtctcatcg aaggggcaag cgacggctcg gggatgggga tcaaattcct
    43141 caagcacatc agccgaaccc tcgggcttgc ctacctgatc gacctcagcg atgatcggta
    43201 tctcaccgcc ttcgacactc tcaccgaaga gctgagggcg ttcgatcccg agctcgtcgg
    43261 aaaggaacgg gtcatcatcg gcacgaagat cgatgaggaa ggaacggatg tgcgcctcgc
    43321 cgaattgaaa gcgaaatacc ccgaggaaac ggtcatcggt ctctgcgtct atttcaacga
    43381 gggagtcgat gcggtaaaag aagcgtttta tcggatcgtg gaacgggttg accctgcgac
    43441 ggtcgaggac aaggggtttc aagcggagat ggatatggat gctcattacg agatggagaa
    43501 agcgccgtgg gaagagtgat ggaaaccgca atggtcggcg gatcgttcga tccgatccac
    43561 cgcggtcact tgcacctcat ccacaccgtg gcttcccgct caccgtatcg acgctttatt
    43621 ctcgtaccgg tgagtcaaaa ccatttcaaa ccggacgccg atcccgctcc ggggagcgat
    43681 cgggtggcga tggccaacct tgcagttgaa gcgtatccgg agatctaccc ggatgatccc
    43741 aagctggagt tcgtcgtcga tgactgtgaa ctgcaccggg aaggtccctc atacacctac
    43801 gataccgtca gggaactcta cctgaagtat ccaatcaagg gacggctcgg cctgctgctc
    43861 ggcgacgatc tgctcgccca attgaagaaa tggcatcgat acaaggagct caaagaactt
    43921 gttcgcttca tcgtcattcg tcgcgaaggt gcgactgtcg agtttgacga tatcgccgcc
    43981 gacctcttct atttggataa tccaacagtt gatgaaagtt cgacgatcat ccgcacggct
    44041 ctcaagcggc tgaatgcaga cgaaccactc gatccggctg tcgaacgcct tatgacggcc
    44101 aatgtcgctt cctatgtccg gacccatcga ctttaccgcg cttgaagcgc atctcactcc
    44161 caaacgcctc aggcattcac tcgccgttac ggccctctgc ctccggctca atgagcgcta
    44221 ccgtctcggt ctcacggacg atgatcttca ggcggtcggt ctcgggcatg atatcgcaag
    44281 gtcatggagc gaggagcggt tgcgctcata tatcgtaaaa catgagatcg ctctgtatcc
    44341 gggggaagag gcggtcgtcg atctccttca tgcaccggtc ggcgcccatc ttttgaagac
    44401 gatgggctac aacaaggatg tggttcaggc gattcgctac cacactcttg gaagcatcga
    44461 gatgggagcg ctcggcttcg tcctgtttgc aagcgattat cttgatccca atcgaacttt
    44521 cttaaccgaa gaggaccgaa agactctcct tgaggaaccg acgatcgaag ctttggtgtt
    44581 tcggattctg gaacgaaacg tccggtatct ggatgccgaa ggaaagcaga tctttgaacc
    44641 gacccgagag ctgctcgccg tactgggggg aaaatgagag cggttgttgt cttctccatc
    44701 tccctgtgtg ttctcttgct tggcggatgc ttcgccgaat cgactgagac aacccgatat
    44761 gaaagtgaaa ccttcaatgc atatgtctgg gtcgaagggc gcgatttggc ggtggtgctg
    44821 gtcgatcgcc cgcttgtggt tcccactgaa cggacggtaa tgccggatca ggatgccctt
    44881 gatcggatta ttggcatcct tcaaaccctc gtcgaagttt ctgaaagctc cgatccggtc
    44941 tctgcgctcc tcgagaacag gaaagccttg agaaaatctc ccttgatgga tacactggct
    45001 ttgatagggg acgatccgca tgcagccacc cgcctgaggc ggttgaacac gacaaccttc
    45061 ctcgatcttc gaggaaaacc gatgaaagaa gatgtgctta gcgtatatct ttccgatgtt
    45121 cgcacctatc aaatgaggag aaagagtgcc caatgaatga acgaacccta agcgatgccc
    45181 aagcgattgt ttcctatctt gaagaccacc gctgtagtga cgtccatatg atcgaccttg
    45241 agggtcgagc ctcctggacc gattgcttca tcatcgccac ggtcaacagt gtcggacacc
    45301 ttcggggagt agtccacaat atctgggatc ttttcaacga gcggggcatc gccgttgcca
    45361 accgccagaa gaagcccgcc ggagacgggt gggaattgat cgactgcggg gatatcatcg
    45421 ttcatctgat gagtgaggaa ctcagggagt tctacgctct tgaacggctg tggcgggaac
    45481 tttaaatcac ctggatctct ttccattttc cccgggcgaa ccgccggatg aaggcgattg
    45541 agcggacgac ccaatcgatg tacattccaa tccatacccc atagactccc catccgaagt
    45601 tcatcgccat gatgtacgag cctccgaccc ggaaagccca catgctgatg attgaaacga
    45661 tcatcgtgaa cttggcgtcc cccgcagccc gaagaaagtt cggaagaacg aaagaggagg
    45721 accagaagaa gacggagacg accatcgatg attggaagag aacggtcgcg atcttggtcg
    45781 cttcttcact gagattgaaa aacgaagcga tcgtgggggc gaggaagaag atcggcgtcg
    45841 caagaatgaa catcgcgata tatgcaaaca ggagaagccg tttggcatag aagaccgaga
    45901 ggtcggtcct gcgagccccg atcgtctgac cgatgaccgt aacggaggcc attgagatcg
    45961 ccccgccggg aatgttgatg aacgtggaga cgcttccggc gatcgcattc gccgcaagcg
    46021 atgcggtccc gaaggaagcg atgaatcctt ggaccagcag ctttccgact tggaagagag
    46081 agccttccac cccggtgggg actcctacga acaggatttt tcgaatcatc caacccttga
    46141 tgtggaagat attgtaggag cggattgtga tgcggtttct ggggtttctc aacagaccca
    46201 acaccagaat cgagccgatg attcgactga tgagggtgga aaggcccgct ccggcaaccc
    46261 ccatcttcat gacaaagatg aaaaaagcgt ttccgccaat attcaggata ttcatgacga
    46321 cgctgatata cagggacacc cggctgttgc ccatcgatcg aaaaagggct gctccactgt
    46381 tgtatgcggc gagaaacggc agggagagaa gaatatacac aaaataggtt gagccgtaat
    46441 cgagcagccg gggttcgatc ttcccgaaga tcgtgctgat gattccacgt ctgatgggca
    46501 tgcacaatag aagaagaagg gtagcgatga gaacggtgag attcaagagc tgcccggcgg
    46561 cggtattcgc cgattcgtca tccttttttc cgaggtactg gctggcgacg accgcccctc
    46621 cggttgcaaa ggctccaaaa agcgtaacga tgagaatcgt gatcgaatcg accagactga
    46681 tcgcgctgac cgccgcttca ccgacggttg tgaccatgag aatatccgcc atcccgatgg
    46741 agacgttcaa gagctgttcc agcatcagcg ggatgatcag cttgaagagg aatcccgatt
    46801 caaaaaattg cccattctca agtgagcggc tcctgttgtg caagatgtat cctcacaccc
    46861 atcctatgct gttttcatca aacgaacaag caccgtttag tcggttgtca gcagggcggc
    46921 gtccgtggca taggtttttt ttcggatatc tttaaagagg cgaaccagct tgtcgacggt
    46981 aaggtctttc ttctcctctc cgctgagatc caggatgatc tggccttcgt ccatcatgat
    47041 gagccgggtt ccgaaatcga tcgcgaactg catattgtgc gtgaccatca acgcagtgag
    47101 tttttgctca ctgatgaagc gttccgtcaa atccatcacg atctgggcgt tcctcggatc
    47161 gagggcggcg gtgtgctcgt ccaagagcag catcttcggt cgactcatcg ccgtcatcag
    47221 cagggtgagg gcttggcgct gtccacccga gagaagcccg acgttgtcct tcagtcggtc
    47281 atccaatccg atctgcttga ggatttcacg aaactcttcc cgcttggcgt tgttcaagct
    47341 gatcttcagg cgtttcatcc ctttcgtttc ggcggtgatc atattgtctt cgacgctcat
    47401 gttcgccgcg gttcccttgg tgggatcctg aaagattcgc ccgatcgtca tcgcccgccg
    47461 gtattccgct ttattggtga tgtcgactcc atcgaaggtg atggttccgc ttgtgaccgg
    47521 ataggtgccg gcgatgatgt tgaacagggt tgatttcccc gatccgttgg aaccgatgat
    47581 gcacacgacg tcacttttgt tgatctgaag gttgatatcg atgagggcgg ttttttcatt
    47641 taccgttccc gggtagaaga ctttggtaat gttctcaagc ttaagcatcg atctcctcct
    47701 gaacgttttt gcgggcaagc gctttcttgc gcgccccccg gctcgatgcg gcgcttcggc
    47761 cttgggcgat gaagaggctc acaatgacca gaataccggt aaggagcttg aagtcattgg
    47821 gggtgatgtt gaccagatag ccatactttc gaccgaagaa gaggagcgcc ttgtagatga
    47881 tcgctccgaa gatcgcccgg agggtgatca ggctgattcg gttggaagag aagaggaatt
    47941 caccgatcat gatcgcagcg aggccttgga caaccatccc ttgaccgagg ttcgcatcgg
    48001 caaacccctg atactgggca agaagtccgc ccgagagggc gatcaatccg ttggaaagac
    48061 cgattcccat cagtttcagc acttcgggat tgattccctg gctgatgacc atctgctcgt
    48121 ttcctcccat cgctcccatc gacacaccca aatcggttcg aaaccatagg tcgacaaggg
    48181 atttgatcac cagcacgacc aacaacgttc cgacaaggga tgcgatctcc ggttggaaga
    48241 aggagaaaaa ggctggaagc ttcgtataga gggtttgaac gcggagcaag gggacgttcg
    48301 ctttgtttcc gagaatccgc aggttgatgg agtagagcat cgtcatcgtc aaaattcccg
    48361 ccagaaggcc ggggatcttc agcttgttgt gaatcgccgc agtaatcatt ccggcgatga
    48421 ctccgctgat aaaggcgaaa acgagggcga tggagattgg aactccgcca acgatcgcca
    48481 tcgtagcgac agccgctccg gtggcaacgg atccgtcgca cgtcaaatcg gcaaaatcca
    48541 agattcgata gctgatgagt atgccgatga ccatcagcga aaaaatcaat ccgtctacaa
    48601 atattccttc tatcatctca tacctctaca cagaaaaacc gccggcaagc cggcggtctg
    48661 agcttcaaca acttagcggc ttacgtgctt gccgtccttg atcagaaccg ctgccgcgtc
    48721 ctgaagggat tgcggaatag tatagccaag ctgagaagct gtatcaaggt tgaaccacag
    48781 ttcatacttc gccggatcag tgatatagac cgttccgatc gatccggctt cggttccttg
    48841 aaggatttgt tcgatgacct tgccggtctc cacgccgatg ttgtagtaat caaaacccca
    48901 ggcgatcatg gcgttcagtc cggcgatggg gctcgggtcg gcgctgaaga gcggtttctt
    48961 cgctttggtc gttacatcat cgatgctggc gatcgcactg acgacggcgt tgtcggtggc
    49021 gacgtagatc gcatcgacgc gatcgatgat cgattgggcg gccatcttca cttcacttga
    49081 attggaaatc gccgcactga cgaattcgcc gccgaacttc tccgttgcgg ctttcgccat
    49141 ctccatcagg accacgccgt tggcttcgcc ggaggcatag acgtttccga tcctcttcgc
    49201 tccggtaacg tccatgagga gtttgatctg ttcttcgacg gggctcttgt cactgacgcc
    49261 ggcgatgttc ttcgccacga gaccggcttc agcggaatcg gttacggcgc tgaagacgac
    49321 gggagtgctt tgattgggac caaagacctg ggcgagcgct tgagcggaag gagtcgcgat
    49381 tcccaccgcc actgagacct tgtcactctt gaacttctga gcgatcgaag aggcggtgga
    49441 gatgtcgccg ttcgcattct gtctgtcaaa ggaaaccgaa aggccttgcg aggtaagata
    49501 gtcgatcatt ccctgctcgg cggcatcaag agcggggtgg gcgatcagct tgctgattcc
    49561 cacggtatat tcagctttgg gggctttatc gctctgtcct gcggcaaata agattgttgt
    49621 gactacaacg aacagcacta gggtggtcaa aatccgtttc ataccggtat cctccaacag
    49681 tgattaagtg gcgttgcgtt actataatgg gttgttgaaa aattgtaaat atctgtatgc
    49741 aaaactatag atattcatac ataaaatatg cataaacaaa aaacacagcc cgaaggctgt
    49801 gctgcaggag aatgtatcac gatttagaaa tcgtcatcgt aatagtagaa atcgtcatca
    49861 tcctcttcgt agagctcatc ctcatacgaa tcgtctccat caaccataaa cccgtcatca
    49921 aggagttcat catcctcttc atcgtcgagc aactcatcgt catcgatcgg gaatctcatc
    49981 gcttcgcgct cgtcgaagtc gtcctcttct tcttcctccc aatcattgaa gtaccgatct
    50041 tcctcatctt ccaattcgtc ttcatctcga tatcgactca tgtcacgctc cttcgtaaga
    50101 cattacatct acaaacatag aagagagtat atcggttgtc aattgtaaat catccgacag
    50161 gtagttgttt gtcctgcttc actccgaggg tgctatgttt gtaggtagtg taagatcaag
    50221 gagtggaaga ccaacaaaac tgatggagaa atagggttgt aaaggaagat ttttaaaaat
    50281 gattctgttt ttgagaccat cctaggtaaa gatggacgct ctgcaccgaa tagtatacaa
    50341 cattcggtgc aagcttgtga cggaaacgga ggaaggggat tcgctaagca agtaaggcat
    50401 atatggtatc cttcctactt tataaacaga gcgtcacggg aagaagcaaa catctccaca
    50461 tcccttcatt ccaagcaatg gactctggac agctcctgac tttcggactt gataagtcaa
    50521 aaatccgctg aaaccattat ctgggggtct gaaatgaaga ttttgtgaga ttttggtctc
    50581 tgtattttct cctactaact cctacttaat atctttacag tctctcatct ccgtgctaca
    50641 ctccaatcaa gtggagagct tgcgatgtat gttggaatag gcgggccgaa gggttatgag
    50701 caggtaacca tccgtgagga tgtctatatt cccgccaccg gcggcaagaa gaccagaatc
    50761 gtcaggaagg tcggcaagct ttccgatctg atggcggagg atccggactt ccttatcaag
    50821 ctgaaagagg aggtacgcct ggagactctg gcgctcaaag aggcagagaa accaatcctt
    50881 cttcccctaa gcgtcgagca gatagagaag ccctcggatg cgacccccac gttcctgttc
    50941 ggcc
//
LOCUS       DAOSLI010000008        46016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_936_length_46016_cov_24.248667, whole genome shotgun sequence.
ACCESSION   DAOSLI010000008 DAOSLI010000000
VERSION     DAOSLI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 46016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46016
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_936_length_46016_cov_24.248667"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>1148
                     /locus_tag="PLF67_02350"
     CDS             46..>1148
                     /locus_tag="PLF67_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88888.1"
                     /translation="MTFTPVDQPRPFRDVLLDAWHNAEGLRGQPDILKINRHIAAASP
                     ELVEEMANIGVQVEVADAKEKSLPASLGSAQKSSRWLMRNHEHHDRSLTGSIQTLCRY
                     AQAEHEFLANNNLKGMNSREVEDRIDEWMTLPVQEPIPMATGGHTWEPGPWLSSWETS
                     LPPDQPRYFKFDGSDGCTWLMTGETAPDKILWDDDFLAYGDYDNAAEIAKNLVDCWPN
                     PPKEIARSVGITLQELQWFIAGKADLDQHARSDLESLLGIEYDDMFGRYAESGPYVLI
                     ARKPQAIKEAYEGISKGGDAFPCEIIPRRGAADPSWRYILINTYDEPPSIVMAPRGEK
                     ITERLPELLLNYDGIRAVSLEFYRDVVSTCVRA"
     assembly_gap    1149..1248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1641..2756)
                     /locus_tag="PLF67_02355"
     CDS             complement(1641..2756)
                     /locus_tag="PLF67_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HOE88889.1"
                     /translation="MSSIWEYESWPHLVYDSPSVSEALERLYKEKQITDIAYSVIDDR
                     TKRQLIAKSLTEDIVSSLSIEGETIDLDSVYSSVSKRLDVVFMGKTKDSAYAQSIVSM
                     VADALENHEPLTHRRLFDWHRNLFENKAGIKPKTIGAYRKGPEYVMRVSGNTHEVIYE
                     AVPPERIQAEMDHLLSFINDRTGLDPFVKAAVASLRFVIIHPFEDGNGRISRAIADYL
                     ISLKAGDAFHAFNISTGILKDRNSYYEQIQAATKDNPGMDVSKWVAWFLTMVSECIVQ
                     SRETLKKTLSTTAFMKSLDPNEFNSRQMSMLYRLADGSFFGKLTTDKWMKLTKCSKTV
                     AFRDIRDLVRKGFLIPSDEAGRNQGYYFNPTDKESYL"
     gene            3190..3999
                     /locus_tag="PLF67_02360"
     CDS             3190..3999
                     /locus_tag="PLF67_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE88890.1"
                     /translation="MKKIMYVMLIMVLVTGGLFAQGTKESGGMVSTDGSTSMEKVIGA
                     LGEQFMADNPGVTFTYNPTGSGSGITAVSEGRCDIGLSSRALKDAEKAQGLKETVLAY
                     DGIAIIVNNDNPVSNLSLDQIAKMYTGQITNWKEVGGKDAPIVLIGREAGSGTRDGFE
                     SITKTKDKNKYRQELTSTGDVIATVGRNTDAIGYASLASVKATVKAVTVGGVVPSEKT
                     VKDGSYVVQRPFMLVTKTGTPLSATAQKFFDYITSAKASSIISASGVVSAN"
     gene            4073..4951
                     /gene="pstC"
                     /locus_tag="PLF67_02365"
     CDS             4073..4951
                     /gene="pstC"
                     /locus_tag="PLF67_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOE88891.1"
                     /translation="MKQSKSLKRTVERVIQTIFLLLGLATVGSVLLITVYIIISGIPA
                     IREIGLVDFLFGKKWASTATPPSYGILPFILTSVYGTGGAILLGVPVGFLTAVYLSKV
                     APKRVKEIVSEAVSLLAGIPSVVYGLVGMLVLVPAIRSVFGVPDGASLLAAIIVLSVM
                     ILPSIVKVSITALDAVPEEYEQASLALGATKTETWFRVSVPAAKSGIAAAIVLGVGRA
                     IGEAMAVMMVSGNVPNMPELFQSVRFLTTAVASEMSYSSGLQRQALFSIALVLYLFIL
                     LINAVFNMLLKGGKKQ"
     gene            4948..5817
                     /gene="pstA"
                     /locus_tag="PLF67_02370"
     CDS             4948..5817
                     /gene="pstA"
                     /locus_tag="PLF67_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOE88892.1"
                     /translation="MSRKTPTLSKRRKAYGIVMQTLMLGATWFTVALVVFLVLYVMIG
                     GLPHITWEFLSTAPSYLTERIGILPDILNTVYIVLATLIFVLPLGVGAAIYLTEYATN
                     RRVVVLIEYAAETLSGIPSIIYGLVGMLFFCQFLNMRTSLMAGALTLVIMNLPTIMRT
                     TQESLKAVPQSYREGAFGLGAGKWRVIRTVVLPGCIDGIITGSILSVGRILGESAALL
                     FTAGFAHAVNGLFKGLSSAGATLTVALYVYAKEQGEFDVAFGIAAILMLLTLIINLSA
                     TVVGKYFQRRKNS"
     gene            5814..6569
                     /gene="pstB"
                     /locus_tag="PLF67_02375"
     CDS             5814..6569
                     /gene="pstB"
                     /locus_tag="PLF67_02375"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOE88893.1"
                     /translation="MKSIITVKDLNLWYSQVQALKSVTMDIPEKSITALIGPSGCGKS
                     TFLKTLNRMNDLIDGVKITGEVRYRDMDIYAPSTDVNELRREIGMVFQKPNPFPMSIY
                     DNVAYGPRTHEIRNRAKLDDIVERALTDAALWDEVKDRLKKSAMGLSGGQQQRLCIAR
                     ALAVEPQVLLMDEPTSALDPISTSKIEDLATTLKSRYTIIIVTHNMHQAVRISDHTAF
                     FLLGDLIEFGNTEKMFSNPQDKRTEDYITGRFG"
     gene            6569..7222
                     /gene="phoU"
                     /locus_tag="PLF67_02380"
     CDS             6569..7222
                     /gene="phoU"
                     /locus_tag="PLF67_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOE88894.1"
                     /translation="MRNKFDEQLALLNRELIEMGALCEEVISMAADALLSGDTELAQK
                     VKPLGGVIDQKERTIEAMCLKLLLQQQPVARDLRQISAALKMITDMERIGDQAEDIAE
                     IIGFLNGRTGRDCAPIGEMATATIKMVTESVDAFVQRDMELAKAVVEYDDVVDALFDK
                     VKTSLIGMIAKHPQDGEYALDLLMIAKYFERIGDHATNIAEWVLFSVTGVHNRENIT"
     gene            7171..7893
                     /locus_tag="PLF67_02385"
     CDS             7171..7893
                     /locus_tag="PLF67_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004057096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE88895.1"
                     /translation="MGLVFRNWCAQPGEYYMIYCVEDDASIREIELYTLRSTGFNAEG
                     FADSKAFFEAIDKELPKLIILDVMLPNMDGVEILTALKADPRTKEIPVIMATAKGSEY
                     DKIQSLDLGADDYLVKPFGMMEMVSRVKAVLRRSEPKESAKIISKDNLTVNLTEHTVH
                     VDGSRVELTLKEFELLRLFLENPGRAFTRDQILSDIWGIDYDGETRTIDVHVRTLRKK
                     LGNASKAIETVRGVGYRLEAHP"
     gene            7962..9542
                     /locus_tag="PLF67_02390"
     CDS             7962..9542
                     /locus_tag="PLF67_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE88896.1"
                     /translation="MSIMYRSLVEEQREQLKNKLAIVANGVEEGGIDWLENLTVKGYR
                     MTLVSSEGVVLFDSEADSATMENHLERKEIADALQSGSGESERISSTMIEKTFYQAKR
                     LENGNILRISFTRNSILSVLLRMLTPVLVILVAAIMLSAFLANRIAKRTITSLNTLDL
                     EHPLNNNTYDELSPLLLRIAHQQRQIESQRSELNKKRDEFSLITQSMNEGLVLLNDKG
                     VIVSINPAARHVFRTDASSIGKDFFVVERSSEIRGVMKTAADSGHGQVKLRRAGRRYE
                     INASRIDFNNEAVGTAILVFDVTEKALLEQQRREFTANVSHELKTPLQSIMGRAELIE
                     NGLAKPGDISHFVNCIRTEAARLLVLIEDIIHLSQLDEGGELPWENVDLYQIAKETLD
                     VLQDAATDKKVRLLLTGTPVSLNGVRGLLGEIIYNLCDNAIKYNNEGGLVEVSVSGDE
                     NKAIIRVKDTGIGIPTEHQKRVFERFYRVDKSHSKETGGTGLGLSIVKHATLLHSGKI
                     EMNSKVGQGTVITVTLPQ"
     gene            complement(9640..10371)
                     /locus_tag="PLF67_02395"
     CDS             complement(9640..10371)
                     /locus_tag="PLF67_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOE88897.1"
                     /translation="MKLAVLADIHGNLRALEAVLADAAQAGAEDIVFLGDLVYNGLDP
                     QMCYELLMKYKPIVTIKGNSDEYLEKIPSFVVNNQEDEQLLKHYKYTSIRMRKKARDE
                     VAAFDLTKQLEICDVNILACHGTPISISDGLYETDENTPILAKKMKEEGIQLVLSGHT
                     HIPADFTVDGVRFVNPGAVGFSLDGDTRPSYAIIECIDGKVRVQFRRVEYDTKKYILE
                     VEHALEGFPLFKFLRYALEKGKAPA"
     gene            complement(10542..11765)
                     /locus_tag="PLF67_02400"
     CDS             complement(10542..11765)
                     /locus_tag="PLF67_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE88898.1"
                     /translation="MAISPFTLCRIKGNERFFYYALFRDPATGKRGNKKSVEVLKKEL
                     GELDGNPVTRRDEAIRICQRALQQGLVFSKPSKLTFYEFLESFYDWSKSEYVKRRNLL
                     QPGSLSKDYIHIRNNMMNNHVLPKIDAKLLLSEVTLDDVERIQLSLVEEKNVGYSTIN
                     VIMSAVGVALGEAQRRGLLDRTKTVAAEPLYVVHKQRGILTEDELVRFMAYAKEHSEK
                     RIYLAILLSLLTGMRSGELRGLKRSALGKDSITVEQAYADHAGLKMPKGKRIRLVPCP
                     SHLIEDLHALAESNPYEGDHDLVFWSKRGGGFVSSHYFSSRFKEELIRSEIFTAEELK
                     ERNITFHSLRHMANTLLRGHVDEHILRLTIGHSSRQLSDLYTHVSQRTLQTVALAQKR
                     TILSLLEDPEHTRPE"
     gene            complement(12307..12777)
                     /gene="smpB"
                     /locus_tag="PLF67_02405"
     CDS             complement(12307..12777)
                     /gene="smpB"
                     /locus_tag="PLF67_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOE88899.1"
                     /translation="MKNSKDHIKMLQKNRKAYFNYEVVEDLECGISLTGTEVKSIRDG
                     RFSFGDSYAAIKDGNLELIGLTIQPYTHGNIHNHEPNRTRRLLAHKRQIRQLRRKVDE
                     KGFTLVPTAIYLRGNLVKVQVSLCRGKQLHDKRNTIKARDMERDAKRELKQLNF"
     gene            complement(12777..>13663)
                     /locus_tag="PLF67_02410"
     CDS             complement(12777..>13663)
                     /locus_tag="PLF67_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOE88900.1"
                     /translation="EYPPQFPYQPVYANLHNDLEALQLRNSQPIIDLALSFISNDTMK
                     SDAERFFPVETKSAPIQAGIQELPPVGKPITLDAGNLTIEGYSYTGSSFIMGDGITPQ
                     SGYAPVSTKDFVLAKRPVSQYEWALFVQENPQWTKSAMNSSSYLAGLSLSPQFSTNRP
                     IYNVSYHAARAFVQWLSEKSGKEVFLPTEAMWSQAAYSQKHTEYDTSLALSERSVPLL
                     GLLGGVWEMTDSHYIPNGRNAGYDRLQELAETYGLEKQVIVKGGSMISKDVTIDSVGV
                     IEPDSVHDYIGFRIGWFE"
     assembly_gap    13664..13763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13764..14269)
                     /locus_tag="PLF67_02415"
     CDS             complement(<13764..14269)
                     /locus_tag="PLF67_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88901.1"
                     /translation="MKQKIDLPPVEEVVLPKLFNLRPGYYLLGLMILVVLLLIFLLGF
                     LPGIRKGGRYVTFEAPLSETGILLDGKYLGSATHQYFVPSGNHTIAYVKADQIYAETE
                     IHVDHPVFLANLIHRTRVIPPPPVTLTEEETASIVSFLLNEVQEISKSLEYPPQFPYQ
                     PVYANLHN"
     gene            14336..15343
                     /locus_tag="PLF67_02420"
     CDS             14336..15343
                     /locus_tag="PLF67_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOE88902.1"
                     /translation="MDRFLTRLESLTVRYLTHRKAVRLYQKMNKPKRTFLGELKGWAD
                     ALVFAVFAVLLINQYLFQLFVIPTPSMVSTLDIGDRVFVSKTIYGVELYPTGPKIGSK
                     NRQVRRDAIITFYNPEYDSKGPLFDILSQIIFSGTLSLVNIDRNEDGSIAERLFVKRA
                     VGLPEELVRFVDGNAELRKAGFTSFEDEASFRKDLDLVSAPHRSIDPALYSGLNAWGS
                     LFGYQEEGLDVNNAPTHLKSSYNSVAHLSFPPDYYTFEKARSETRHLLDPTNLGLRSE
                     ASHYRRGTYVPKNAILPLGDNRDDSRDGRYFGPVDQSTINGSVVFRFWPPNRVRPMGD
                     A"
     gene            15343..16494
                     /gene="hemW"
                     /locus_tag="PLF67_02425"
     CDS             15343..16494
                     /gene="hemW"
                     /locus_tag="PLF67_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOE88903.1"
                     /translation="MGSLYIHIPFCTSRCGYCTFYSELYHTHASLVEPYFKRLLEELS
                     FLVHEKRLSFDTVYIGGGNPGLLGVERLFSLVKLIHTRGKPREVTMEINPETLTEEFF
                     PLFDDRLITRLSIGIQSMNDGFLKRIGRNASSLINERALRLLERIRTLYDVEVSVDLI
                     VAIPGQTIGDLQSDLKRILSLCAANHISLYCLTVEEGSRLFEQGDIFMSEDEQASLLT
                     ELWAYLKTLGFEHYEVSNFAREGAYSRHNLVYWKLGRYIGLGSSAASTIEEEGALVHY
                     AQEQDLQAYAQGEPFTGYEKELLTPVERIEEEIMVGLRTRWGIDKDSFSRRTGYDFST
                     LFDRIIATLDPAWYTESSQFFVLTENGFMVLDAILLRFFEAIAEGLDRP"
     gene            16550..17293
                     /locus_tag="PLF67_02430"
     CDS             16550..17293
                     /locus_tag="PLF67_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE88904.1"
                     /translation="MSGHSKWATIKRKKGAADAARGQKFTKLIKEISVAAKNGGPDPD
                     SNAALRTAILKARSENMPKDNIDRAIKKGSGELENSVFYELTYEGYAPGGVAIIIDTL
                     TDNKNRTASEVRSTLTKGGGTLGTTGSVSYMFQTKGMIIYDDQKYTEEEIFEAALDHG
                     ADDVLHEDGMIEVYTAPGDFANVLESLQASGFEEESAEIAKIADQTVELDDERTRKVL
                     KILERLEELDDVQQVFSNLEIPDGFEDEE"
     gene            17298..17789
                     /locus_tag="PLF67_02435"
     CDS             17298..17789
                     /locus_tag="PLF67_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOE88905.1"
                     /translation="MRILGIDPGFAQTGWGVVETTRQHTRLVSYGVIRTTTKENDSSR
                     IHFIAKHVGLLAVEHGVEICGMEDIYFAKNISSAIPVAKVIGSIMHELTAREIPIRFF
                     SPPVIKSSVVGYGGADKTQVQEMVRILLSLEKIPRPDHAADALAAALCLQAHETTRIR
                     MNS"
     gene            17789..18409
                     /gene="ruvA"
                     /locus_tag="PLF67_02440"
     CDS             17789..18409
                     /gene="ruvA"
                     /locus_tag="PLF67_02440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOE88906.1"
                     /translation="MINALIGELVSVKEGSMIVRSNNIEYVLLISNQSASRFSSLDKE
                     ERKLIKILTSLQVREDSLTLYGFYDDAEREAFEQLQTVSGIGPKQALKILSGINVRHL
                     AEALDSGNIKTLATIPGIGPKTAQKMVLALRNVLVLDEPKMKQTTSRTAVWADVVNAL
                     VEMGFERRRSEEVIGDLEVEFAREIGELSRQSAEELLFRHALKNLS"
     gene            18418..19470
                     /gene="ruvB"
                     /locus_tag="PLF67_02445"
     CDS             18418..19470
                     /gene="ruvB"
                     /locus_tag="PLF67_02445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOE88907.1"
                     /translation="MDHYNQLLENSVVSASFQEIADRQENVLRPKMLSDFQGQARLKE
                     NLSIFIQAALQRGEALDHTFLIGPPGLGKTTLASIIANEMGSEIRMTSAPALDKPKDL
                     AGILTNVTENSIFFIDEIHRLKPALEEMLYIAMEDFEIDWIIGQGPSARTMRIPLPPF
                     TLVGATTKAGSVSSPLASRFGITAHIEFYNEEELANIIDRSSKIIDVRISEDAVLTLA
                     RCSRGTPRIANRLLRRLRDFASVLGEGTITSEIVHTGLKRLGIDQQGLENQDRNILRA
                     IIEHYDGGPVGAETLSISVGEALESLEDFYEPYLIQKGYLKRTPRGRMATSKAYELLN
                     IKKKRNTPDDDQGLLF"
     gene            19445..20467
                     /gene="queA"
                     /locus_tag="PLF67_02450"
     CDS             19445..20467
                     /gene="queA"
                     /locus_tag="PLF67_02450"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOE88908.1"
                     /translation="MTIKDYSFDLPLELIAQRPSEVRGEDRLLVVDRANDTFFDDRIS
                     SFPDLLEEGSILVLNNSKVRKARLYAESESGGIVEFLFLSENPDRTWNAFVTKSKRQR
                     VGKRYRFQEHEATIVAEQSDGSKVVAFASPIDEAFFSRLGHVPLPPYIKRTDDPDDET
                     RYQTIYAQTEGSVAAPTAGLHFTEDHLKRINERGIEIASVTLHVGAGTFLPVRAERLE
                     DHHMHTESYEINEAVACKVMRAKREGRKVVAVGTTSVRTLESAYDEQSGMIRSGFGKT
                     DLFIRPGFHFHVVDQLLTNFHTPESTLLVLVSTFASKGLMDEAYRHAVSERYRFFSYG
                     DAMFIR"
     gene            complement(20457..21068)
                     /gene="tmk"
                     /locus_tag="PLF67_02455"
     CDS             complement(20457..21068)
                     /gene="tmk"
                     /locus_tag="PLF67_02455"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOE88909.1"
                     /translation="MSTILKNFIVFEGLDGSGTTTQMQLLAETCDRNSRLCKATFEPT
                     DKPIGRLVRSILQKQVVTTPLALALLYAADREDHLFNPIYGIASEAEAGRLVISDRYL
                     YSSLAYQSVECGYETVAALNQFPHPEYLFFLDTPVDECLRRIAHRKTETELFEHQEFL
                     IQVKANYERIFEDLDPRVKLTRIDGLLSIEEISEIIKNTIFNG"
     gene            21167..25765
                     /locus_tag="PLF67_02460"
     CDS             21167..25765
                     /locus_tag="PLF67_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOE88910.1"
                     /translation="MRFHTRAKDIIAATSILLLITAIVGWVLIFDEPTLSPASLLGDY
                     LLSRLSTEGASNLSFTSIERNLFANLTINNLSYRRGKDLSITIDKTNLEGGLFNLFLS
                     LFSSKPLLSLSFQEPKIYLGPNHKPGEEANAVPRLLRSWVRRLQLEALGTKGSFEAEI
                     PFGEIEVADIDVALHWKLSDELPSFTIQARRLAALTPSFTLQSSHLLASLGHSGPFTF
                     KGLDTAMDANDQRVTIANLSVEGEVVSLKDRSLNAQATLRQGLYQTGDVTVETPELSF
                     SGRYLDDRLPTVSLSVPSVFVNTPDLQAEGSHVDLHIYDLTTLLLRSERIDLTALETA
                     LTLLGIEVHLDAQENNLYYVTSTLNEGRISALGHTLAVHAFETLGQGMYQAGTLERVE
                     LQSSALGEVPNLFGIRFPLETSFLYDRSHEHIVGRAFSQALASDLFTEAFTFSLSYER
                     DRNDHSLDVLIEQKADLRLLAHAERMSDHLLDFSLTGRFNHFSLGVIEPLLAEYAPFL
                     NPYYTQDTALTGNLFFTARIGKGNVLGLDGRISLDGSLSTVRLGRFESSAGFLFTAAV
                     VEDLVDVRSAALSAEGYQLSYSGEINLTDSFTQGSARLLQIDDNQLLATLGIRGRMNH
                     VLTLDFSTPLVRELAVGMDVSLKDQRLVHGAGEVHLFDMDYPFTLNVDLSLLEFSLEQ
                     EGALRISGTLMPIITIAFDAFYFTLPISRTSISGKALLSIDHYSSFLFESEQISIHNL
                     AVGPTVYSIDAQLSVSSDELFIRNARFTDWVNTYSGNAHYQGSPLFSLLKHRFLQPFS
                     FNLSIFRDESPIIQTTLIGSSNRLQSMITTNGLSLGSFVSALEGTYLDLTLLGSSDLS
                     SEINLSGSAELRSDAFSLSTKIGAEGDNFRLYDSFFRWKELTFSGDLLSIANEEARLL
                     GSFEHIRHLSYIDQLSRLDVQVGVALPGVQNLFMLPDRAKPLLTQGIGTAWITIGEPL
                     IYGEGGFTGGTHVLDSDSTALIVDGSFLRGRYEYRTRTLSARLDPSFGIGATIRADLR
                     PESFGVKVEDIVFPLPYLNRTYLKPVFAFLEGTANGTVFFTGSMQSPRIYGQLSVDSS
                     QMTTFWVYDDIISLKNLSVSIDGNRAISPHLPFFSTNRKTGNVTTGTALLRAQLGGLG
                     LDHYEITIESIDDPIYLWIPMLGYDADLKMWAEGSFNLWGKGLETWLSGDLIISDAVL
                     SLGVRGLPYWYTHEKGTSNDFNLTTDKGVTFLYPNSTNPIIKATISEGEKLSFTYDHL
                     TDEITMDGSLALRSGEIYYFQKDFFITEGALTVHTESFGGLPSVQPTINLRAKLTDYD
                     SSGNRVDIFLILREASFTALNPQFESIPAKATNEIFEILGQSILPSGAYGQFNLYSAA
                     HLAAAATNVAERLGYINVFQTSALTETIRVSLGLDMFSLRSNILQNILLDTLPGATPG
                     TTFSPLARYLNNTVIFMGKYIGTEFFLQALLHLSATEPNKIRNPFLAPDLSLNLELSI
                     EWHNPLLTFSLFTQPNELSFIDILDTMGVSVTKRIVLR"
     gene            25785..28337
                     /gene="bamA"
                     /locus_tag="PLF67_02465"
     CDS             25785..28337
                     /gene="bamA"
                     /locus_tag="PLF67_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOE88911.1"
                     /translation="MKSLYRRILWIALLILLATVNIFASDAEPWYMGRPIASFVNTGL
                     LNVRESEILDIQYEYLRKPFSDQLFNELQSKLYALEYFYYFLADAERTGEGGNELSIV
                     MHFEELPYVNEVLIEGNSGLKTKDILGASLVQAGSFFDEYTLTLSRDLIEQLYREKGY
                     INAQIERTYTEDEATNTVSILFEIEEGSQSRIGEIAFEGVHSVPPASLAKQLESKSVS
                     LFNPGYYNPLLEETDKKAIIQYYQNRGYIDVEVGPAYVEDISSDDDKYTRLRLVYPIA
                     EGSQWFFGGIEVSGNEVFSDEQFQNLISIKTGSVLDLSRIQAQIAALTDLYWNNGYIF
                     NLISSDEVRNEENKTITYRLHVQENRQAIIEDVRIEGITRSKPYVFERELAFKKGDIF
                     SKELLIKSAQNIYNTLIVTDVQFDILNGTEEGTVIPVFTVTEGNQMDLQFGATFGGNV
                     DGFPVSGFLQWSNKNLGGTGRDLSVSTNLSPDTQTVMIAFQDDWFRSYRWSNGFTFSF
                     ERSVKENTLTRGQGSGYFTTSTSANTYPNGYESYQAYQAAGQAYPTADKLMEYVQFRF
                     SLGYNTGYTFMFRPGALSIGVGVSIGLNKAEYDKAIYDPYEWLIKQYGEKWQFSNRLS
                     LTFAWDGRDRIENTSRGYYISETFTYAGGILFGLSNYMRTTTSLSGYLTLFSFQLEEK
                     PANVVLGMTTTVGAMLPQFHNPVTQDGEQGWGWYDAKMGATRYEMLYLDGMNTARGFP
                     VVFDQAFLWDTQLSVSWPLVHNVLSAELYISASGVSQSLKETKMDQLAWYFSAGGGIK
                     LKVPGFPLGLYLVKNATYIDNTFAWDTNHFLFRGQNDTSGLKLILAITTTLY"
     gene            28339..30912
                     /gene="mutS"
                     /locus_tag="PLF67_02470"
     CDS             28339..30912
                     /gene="mutS"
                     /locus_tag="PLF67_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOE88912.1"
                     /translation="MSKTQRTDTPMMAQYRSIKEQHPDSVLFFRLGDFYEMFEDDAIE
                     VSRLLNITLTARGGVPMAGVPYHAAKNYIKRLLDVGKKVAICEQTLTGEKLAQREVVQ
                     VVTPATVIEDDFLQAHDANFLMAATIRKGSIVTSYADLGSGRFFVRQVEHDPGYIHLL
                     ALLEEVRPRELLVNEDEYFLLGEFKEAIDQTSAMVTKLPPYQFSAKKAYESLLEHFEV
                     HSLKAFGIDDHAPYLSSAGALLSYLQQTARSSLKQIETLTLVHSAEFLQIDEDSRKSL
                     ELFANLSDGSPRYTLFDSIDSTVTSMGTRKLKQWVASPLARKETIFSRHRWVDLLYHD
                     REELERVRHELKQVLDLERLTSRVVMGRHLPKDITGIAQALRGFFLLFDERYRDLLVT
                     TQQEHEKAFALATHIDRALNPTHQGPFEEGRVILDGYDSELDELRKIKGGGKELLAEY
                     LEEIKVQTGIPTVKLGNNRILGHYLEVTKIHSDKMPATFYRKQTLVNAERFTSDELIA
                     LERKIQHAALDAEQYERALYEHLVEETLAAQKTLLAIAAFLSDLDCYQSLAYRAHISS
                     FVKPEFVDEDCLIVEGGRHPVVEANLPSGEFVANPLTIEKEHRRFCLITGPNMAGKST
                     YLRQNALIVLLAQIGSYVPAAKATLSLVDRLYCRVGASDNLARGESTFLVEMLEAAHI
                     LRTATSASLVIIDELGRGTSTQDGMSIAYAVMRELIHIGSKTLFATHYHELARTEEQN
                     LQLLTLEVLEQGKEIRFLRRVIEGVANSSYGLNVAKMAGIHPSILKNAQSFQRQHFAD
                     YDLSSLQMNLFSEREDAPSSYNEVEEQVLVDLESFAIEHATPVDALLFLKELQERLKA
                     K"
     gene            30935..31522
                     /locus_tag="PLF67_02475"
     CDS             30935..31522
                     /locus_tag="PLF67_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88913.1"
                     /translation="MYCIGCDRISDELLCPSCDGKIQAGSYAYLYDRRCPDCHLPIFD
                     SAYPCSWCTDGRIGWGGYEGLLARLLTLHKSRKDRRLVHYLAKLLVPMMNMIAPDLFI
                     PVPASEGGCRQRGYDQMVLFSKELSRQMGIPSRPVLIQKKGAIVLLTRNMRIENKRIV
                     LLDDIYTTGKTSSDCRKILKETWGIDPFVVTIAES"
     gene            31641..32141
                     /locus_tag="PLF67_02480"
     CDS             31641..32141
                     /locus_tag="PLF67_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOE88914.1"
                     /translation="MLSKGIQETPLFVTFDPLIKAVNLRLVDLSVFESRGHVGVNLVI
                     MAENRNTTVEDCATVHKIVAPRLNVLYDTREVGIEVSTPGLQRNLKDVYEFTLYSGKR
                     VRIYSTILKGWISGRIESVQENSLTLNDVFVEDTKEQLERKSVEFDEIQKAKLDYRWE
                     DRPHGN"
     gene            32131..33624
                     /gene="nusA"
                     /locus_tag="PLF67_02485"
     CDS             32131..33624
                     /gene="nusA"
                     /locus_tag="PLF67_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOE88915.1"
                     /translation="MATDIGDAVRAMVAEKNISEELVISTIEDILRAAFKRKFGTDEN
                     AVIQFSDDFSTVELASRRLVVDDDNWYNEVTEIPYSEAVGLHEGATIGDELLIPVDLK
                     DFDRISVQSAKQRAHQSFRDIQKDTLYSEYKAKEGMVIIGYFRRQLPNGDIYVDIGNT
                     EGLLPKRNQSSRESYQSGDKIKCYVEKVEKAERGLRVILSRSSPELLRRLFEVEVPEI
                     AQNQVDIIKIVREAGYRSKVAVASRNDEIDPVGACVGLKGNRIQTIMSEIEGEKIDIL
                     RYDPNPVNYIRNALSPAQVRDVVIIDKNIYHAVAIVDESQLSLAIGKQGLNVRLANKL
                     VDWMIDVKTQEQFEEMDIAKEARYRAESIFIDEDGQQYYSDEQPVHFEEPMFAEEERM
                     DLGIDEDEIALTELPLGEELITKLHFYDIYTVEEFINISDEQLATLENLTAEELIRIK
                     DTITEHVDIVEDDDEEMQYICPSCGGIITEDMDICPHCGTGLAFEEE"
     gene            33640..36204
                     /gene="infB"
                     /locus_tag="PLF67_02490"
     CDS             33640..36204
                     /gene="infB"
                     /locus_tag="PLF67_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOE88916.1"
                     /translation="MTEEKKKPKITLIKQVVAPNEPVEETKSTSDKEGEQKPKTTEKR
                     RVVVVKKKVVKAKPAPKPVPSKSEPRKKQDEKSAAPPAARTIVRKEPVVSERLGSSPL
                     HTGPKVIRPTNLPPVPNQGLTINDTPQRVQRPPQPQQGRRVYGTVGGRPARQQEGSRA
                     PRQGGGQRSSQRPPYQGGGQRQDGYVQRPPQGGGFTPRQGGRPGGFRPSPMGEELPPQ
                     DHQRPQKKTFTKRKDQTYKRRDAEEEREFQIQRRKEQAAAKLAAVPKSIDMMEVITVA
                     DLAKKMNLKAAEIIAMLFKMGMMVTINQQIDFETAQIICNEYNCDVRLVSLYDETIIA
                     SDEDENEELVERAPIVTVMGHVDHGKTKLLDAIRSTKVAEGEYGGITQHIGAYKVNLP
                     GKGDVVFLDTPGHSAFSMMRARGAQLTDIVILVVAANDGVMPQTREAIDHAKAANVPI
                     VVAINKIDLPDAKPERVMQQLSDLGLMPEEWGGQTLFCQISALQKLGIEELLDTVLLQ
                     AEMLELKAAVNCRAEGKVLESRVDQGRGIVASVLIERGTLRQGDYYVAGIYAGRVRAM
                     FDDTGNKIEEAGPSTPVEIIGLSDIPGAGDPFQVTEDERQARQVGAKRQELERLGESR
                     NVKKVTLESLYSKIQEGEIQDFNVIIKGDVQGSVEALQQSLEKLSNNEIRLNVIRASA
                     GAIIESDVTLASASDALVIGFNVRPTPRAQALADQEKIEIRKYNIIYDAIDDIRSAME
                     GMLAPELREIEIGTVEVRETFKVPKVGLIAGCMVTKGKVKRNSYVKVIRDSVQISPER
                     LKISSLKRFKDDAREVIEGFECGIGLEHFHDLRVGDILEIYEHKEIARKLELNGQV"
     gene            36201..36563
                     /gene="rbfA"
                     /locus_tag="PLF67_02495"
     CDS             36201..36563
                     /gene="rbfA"
                     /locus_tag="PLF67_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOE88917.1"
                     /translation="MSDFVRQRLEAKIAEAISTLIMKGEIKSPELSTLVSVSRVELAP
                     GNGSATIYISSVLDDASLNQSVEALRRAGGFIQKRVGAFLKTRNTPVLTFKADHSLAD
                     GHKINTLIDSLMNDEHGK"
     gene            36547..37407
                     /gene="truB"
                     /locus_tag="PLF67_02500"
     CDS             36547..37407
                     /gene="truB"
                     /locus_tag="PLF67_02500"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOE88918.1"
                     /translation="MSTASRIILLNKESGVTSFTALNKVKKTFGRKVGHAGTLDKFAQ
                     GLLIVLTGAMTKLNPLFSSMDKSYRAHILFGSETDTLDPEGRVIATGEIPSFEAVREA
                     IQSFDGEMLQTPPIYSALHVGGKRASALARKGKPVELESRPIRIDSFEVVSYEDGILI
                     ADIRVSKGTYIRSIARDLGLAVSSRAHLVALVRTSIGPFALSEATEVDEQVDLTNDYL
                     SRLEQMALATVSDDQLFPLANGRYPDHYDLMKEGSDYIAFYSGDGVLRAVGNKTKRRL
                     IAQVYRGGAV"
     gene            37404..38231
                     /locus_tag="PLF67_02505"
     CDS             37404..38231
                     /locus_tag="PLF67_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOE88919.1"
                     /translation="MKIHMFMDLSRTKILKDVPMVVSVGVFDGLHEGHLSILRRVKEI
                     ADSNKMESMVITFDRNPKMAAKSIEHHDQLMTDRQRNELLASLGIDHLAIIDFCPEFS
                     KLTGEEFLALISGLCRLEAMVVGEDFRCGVPTSSVGSVQLPEHLNRLSPGSFVEIPPF
                     VLTKDGEITSSSLVRKKLLEGALEGVQSMLGRPYTLDLVSHTPISTVKGLLYRTESFS
                     QLLPKEGVYDATLAYADSREVDLVAHVMAEGVLLSSMAESDADGISYLSLYAKGSRT"
     gene            38228..38494
                     /gene="rpsO"
                     /locus_tag="PLF67_02510"
     CDS             38228..38494
                     /gene="rpsO"
                     /locus_tag="PLF67_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014024011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOE88920.1"
                     /translation="MITKEQKEEIVVKFGGDAKNTGSTEVQIALLTARINDLQDHFKN
                     NPKDHAGNRGLLKLVGQRRRLLKYLRNRDIEAYRTLIAELGLRK"
     gene            38590..40704
                     /gene="pnp"
                     /locus_tag="PLF67_02515"
     CDS             38590..40704
                     /gene="pnp"
                     /locus_tag="PLF67_02515"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOE88921.1"
                     /translation="MMESKTVRVRIGDSDLVFETGKIAKQANGAVYASYEGSAVLATV
                     CSGPVPNEELDYVPLSVEYNEKYYAAGKIPGGFLKREGRPRDKEILVSRLIDRPMRPL
                     FDKAFGREIQVIPTVISSDMNSTPDIIAINAASCAVVISDIPFNGPIAAVRVSQVAGK
                     LVINPTFSQIEESTLDIVVAGTRDGITMVEGAAKEVDEDLMLEAIEAAVPVIRSIVDA
                     QLELAKIAGKEKLPLPVFEKDLSFLQPIKAFAEPKLKEASFVKGKMERHAAIHQVLEE
                     TLDKFASVLEEDAKREKEVRALFEELEYTILRSSILNNGVRTDGRTTTQIRKITSEVG
                     LLPRTHGSALFTRGETQSLAVTTLGTTSDEQMFDTIDGEKSFSSFMLHYNFPPFSVGE
                     TGRLSTGRREIGHGNLAQRALESVIPSKEEFPYTIRIVSEITESNGSSSMASVCGGTL
                     SLMDAGVPIAKPVAGIAMGLITKDADYSQYAILSDILGEEDHLGDMDFKVAGTRDGIT
                     AFQMDIKIAGITPKVMAEALAQAKDGRMHILNEMDKTLSNPRKEISEFAPKILTMKVD
                     EDKIGAIIGTGGKTIKGIAAQSGAEVNIEDDGTIMIYGRNNTSAQKAKELVTSIIEEP
                     EVGKIYQGTVKRIMDFGAFIEILPGKEGLCHISKLSRSRVQNVEDVLTLGQVVPVKLM
                     EIDRQNRLNLSYIDAIDPKESK"
     gene            40701..41138
                     /gene="dut"
                     /locus_tag="PLF67_02520"
     CDS             40701..41138
                     /gene="dut"
                     /locus_tag="PLF67_02520"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOE88922.1"
                     /translation="MIAVKVLALKAGASLPAYQTAGSAGADLKALLDEPVVIEPGNRA
                     LIPTGIALELPVGYEAQIRPRSGLAAKAGLTVLNAPGTIDSDYRGEVTVILINHGSEA
                     AIVRNGDRIAQLVIAPVVQASFSPADALDSTERGSGGFGSTGV"
     gene            41144..42355
                     /locus_tag="PLF67_02525"
     CDS             41144..42355
                     /locus_tag="PLF67_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOE88923.1"
                     /translation="MAKRYALVYRHVSREYLLSFFVAFLFFFFIFFINQILLLAQRIL
                     IKQIDYYSVFKVIIYSIPQFLLFTFPFSSLTASSMTLGDLSEHREILAMRCNGISPIR
                     LYLPILLLSLIITAATFVTADYVLPVSSARYRELYISLMQDLPTLELVDGGINTIGGK
                     TLINKHVNENRIDELILFDSSSREDGKILTAPSATVTLDDPLNFVYLLELDRPRLLYT
                     RSGGEWALAQAERARLFLDFSKQVGSLGTVLPSQLSSKQLQAEIAQYRISFDEEMKHY
                     NERLSALREEVKAGTKSKAELKEFKKQKPINFYLQYYTAELHKKYALSTACTILLFLT
                     FVLAHLRLRHGKLIGFGLSMITAVIYWYLLFFAQLQIFTYPISAALFIWAPNIILGTL
                     GILLLLIMRRS"
     gene            42352..43434
                     /locus_tag="PLF67_02530"
     CDS             42352..43434
                     /locus_tag="PLF67_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOE88924.1"
                     /translation="MKLYRRYIFKSTINVGLGALFLTTLMLISVDLFANLDTYLSHEL
                     PLATIAYLTLLGAPQAVLFALGPSLLFSATFFLSQLHANNEYICLIGSGIRPLKIILP
                     IIAVGFIASILQFAFAEYVKLPLSKERLILEEELFGLRSTQDNRNITLHDPEGRYVLY
                     ARHYRAQDNLLREVLLLLFDEQGNLTHRLDAQQGRYDEDSGQWRFHGVQSQEVGEVVS
                     TRTLEAYDNPRINLDPSYFRGAGGDIDQMDIQTALQYLHWMKRLDPNRHTPLAADFVK
                     RILDALNPLVLIVIAATIRYSLKKNILLFAIITSLIVAVVYYVVQMVTMIAARQGVLS
                     PVWAMVIPSVVVLLLAALQRTLSRQG"
     gene            43437..44555
                     /gene="tgt"
                     /locus_tag="PLF67_02535"
     CDS             43437..44555
                     /gene="tgt"
                     /locus_tag="PLF67_02535"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOE88925.1"
                     /translation="MAIFETTHQDAKTAARLGLLHLGHGTVETPVFMPVGTAGTVKGI
                     HHEEVERIGYKLILGNTYHLYLRPGLEVLKQYGGLHRFSAWNHNLLTDSGGFQVFSLS
                     GLRKIEEKGVSFQSHIDGSRHLFTPESVIDTQAVIGSDIAMCLDVCTPPEISYRAAKE
                     AMELTHRWAQRAIVHRNQHETMKGNLFGIVQGNFYEDLRKASAEFFNEQDFPGLAIGG
                     LSVGEPKEEFIRFLAHTASYMTKEKPRYVMGIGSPDYILEAVEQGIDMFDCVLATRMA
                     RNGALFTDDGILVMKKAIFKFDQGPIEEGCTCRACTHYSRGYLHHLIKAKEMLGGMLA
                     TEHNLTYFHRLMEKIRNAIANDEFASFKRAYLERFYGT"
     gene            44503..>46016
                     /gene="murJ"
                     /locus_tag="PLF67_02540"
     CDS             44503..>46016
                     /gene="murJ"
                     /locus_tag="PLF67_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOE88926.1"
                     /translation="MSLPLLSEPTLSDSTEHDKQSLTVMICTLASRLLGIVRSRVLTT
                     LFGATAVADVINFTFNIPNNFRKLFAEGAMNSALIPSFSSLLAEKRNNEAQKLFALLV
                     TYQTVLFIPLTVFTYIYGKEIIAFLSDFEAEQVLLGSKLLPYFIAYLGTISLSATFTG
                     LLQLHKRFFHAFASPLCYSIVVISGVLLTSSSFGAMSVAYATIAGGALQGFYAYITLR
                     REGYRFRLMFNPKGTTFAPTLKAWGFVSIGMGMQIVTQMVSYYLASTLGEGSVTAFAN
                     SLIFYQTPYGIFFNAIAAVSLPALSMAFAQNDHAALKRQSREALSKLTSLLLPSTIII
                     YAFRLEIISVILQGGKFTHADSIRTATVLGPYLLFMTSTAFHSLLVRLGFATKRFKAM
                     TMVQIVQNVLDMLLMVLLIKGNAGLQALSLANGISYLLSLFIILFIVKDLYTPFTDRV
                     LFKEVGKIILANIPILVGAILYKLLAPPWYADGSTWKNLGILIVIGLSSIFVWLT"
BASE COUNT        11144 a        12242 c        11905 g        10525 t
ORIGIN      
        1 ctcatactct atggattgac cgttgccggg ttgccccttc gctggatgac ctttaccccg
       61 gtcgaccagc ccaggccttt ccgggatgtt cttttggatg catggcataa cgccgagggc
      121 ttgcggggtc aaccggatat cctaaagatc aaccgccaca tcgcggcggc cagcccagag
      181 ctggtagagg aaatggcaaa catcggagtt caggttgagg ttgccgacgc caaggaaaaa
      241 tcccttccgg cctctctggg ctcagctcag aaatccagcc ggtggctgat gaggaaccac
      301 gaacaccatg accggtctct taccggatct attcagaccc tttgccgata cgctcaagct
      361 gagcacgaat ttcttgccaa caataacctg aaaggtatga atagccgaga ggttgaagac
      421 agaatcgatg agtggatgac cttgccggtg caggagccga taccgatggc gacaggtgga
      481 catacttggg aaccgggtcc atggctgtct tcttgggaga cctctctgcc gcccgatcaa
      541 ccccgttact tcaagttcga tggctctgac ggctgcacct ggcttatgac aggtgaaacg
      601 gcaccggata aaatcctctg ggatgatgat ttcctggcct acggcgacta tgacaacgca
      661 gccgaaatcg ccaagaacct tgtggactgc tggcctaacc ctcctaagga gattgccaga
      721 agtgtcggaa tcaccctgca ggaacttcag tggttcatcg ccggaaaggc tgatcttgac
      781 caacatgcac gttctgatct ggaatcgttg cttggcatcg aatacgatga tatgtttggc
      841 agatatgcag aatccggccc ctatgtcttg atagcccgca aaccacaggc catcaaggaa
      901 gcctatgaag gcatttccaa gggtggagat gcatttccct gtgagatcat ccctcgccgg
      961 ggagccgccg atccaagctg gcggtacata ttgatcaata catacgatga acccccgagc
     1021 atcgtgatgg cacctcgtgg agaaaaaatc acggagcgcc ttcccgagtt gcttttgaat
     1081 tatgatggca tcagggctgt ctcgctggaa ttctaccggg atgtcgtttc cacctgcgtc
     1141 cgggcatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gctgtctcgc
     1261 tggaattcta ccgggatgtc gtttccacct gcgtccgggc atgtcgcgaa ccttccgcca
     1321 acgtttggga gatgaaagag tttgggaaac gctatgaaga acactggacg gattgcttct
     1381 gggagccgga ttgaaccttc aaagcgcggg gattgtgatc agacgaatcg ggatcgcgac
     1441 aagatcaccg gaatcaatcc tgcaacgatc atccggagaa gctcggcatc gtcaaggaac
     1501 tgaccgcccg gaaacgcaac cgcctgttca gctatgccgg ctatatcgag atcatgagat
     1561 gcggcacgga actgccgggc gggtaggccg gtaaaaccgg actttcagaa tcgaattcct
     1621 tgggatattg tttttatctg tcaaagatag ctttctttat ccgtcggatt gaagtaatac
     1681 ccctgattcc ttcctgcctc gtcggaagga atcagaaaac ccttccgaac cagatcccgg
     1741 atgtcccgga aggcgaccgt ctttgagcac tttgtcagct tcatccattt gtccgtggtg
     1801 agctttccga aaaacgagcc atcggccagt ctgtagagca tgctcatctg cctggagttg
     1861 aactcattcg ggtcaaggga tttcatgaag gcggttgttg aaagggtttt cttcaacgtc
     1921 tctcttgatt gtacgatgca ttctgaaacc atggtgagaa accaggcgac ccactttgag
     1981 acatccatgc cgggattatc tttcgtagca gcctgaatct gctcataata ggagttcctg
     2041 tctttgagaa ttcccgtgga gatattgaac gcatgaaaag catccccggc cttcaagctt
     2101 atgagataat ccgcaatcgc ccttgagatt ctgccattgc catcctcaaa cgggtggatg
     2161 atcacgaacc ggagggaagc taccgccgct ttgacaaacg gatccaatcc cgtcctgtca
     2221 ttgatgaagg aaaggaggtg atccatttcc gcctgaatac gttcaggagg taccgcctca
     2281 tagatgacct catgggtgtt tcccgagact cgcatgacat attcaggtcc tttcctgtat
     2341 gctccgattg ttttcggctt tattccggcc ttgttttcaa aaagattcct atgccaatcg
     2401 aacagtcgcc ggtgggtcag cggttcatgg ttttccaacg catccgcaac catggatacg
     2461 atgctttggg cataggcact gtccttggtt tttcccatga agacaacatc aaggcgtttc
     2521 gaaaccgatg aatacaccga atcgagatcg atcgtttcac cttcgatgga aagggaggaa
     2581 acgatatcct ccgttaagct tttagcaatc aactgtcttt ttgtccgatc atcaatgacg
     2641 gaatatgcga tatccgtaat ctgcttttct ttataaagac gttccaaagc ttcggaaacg
     2701 gatggactgt catatacaag atgtggccat gattcatatt cccagataga gctcatgaga
     2761 gcattctatc attccacttt tgaaatgaaa agagctttcg tttcatttat ggaacgaata
     2821 tcatgaatat tccatcaatg aaccgaatgg gttttatatg agaagaacga tgatcatgat
     2881 tttatacact caataatgaa cccccatggt tcaaagctga aaaacagatc accattcgtg
     2941 tcgacatgaa tccatatctg agagactgtg tcgacaagaa aagaaagccg gttttccttt
     3001 gtccgggcag gttcaatgta aatactttgc aattactttc cgtatcgata gataccccct
     3061 ccttttcaac tgagagggat cgctttcgct ttacctcgat ttaacatgac ttgtcttttt
     3121 catttaacat tcgaacttta caatgaggcc atgaaaaaag ataacatcaa taaaacagga
     3181 gaaaatacaa tgaaaaaaat catgtacgtg atgttgatca tggttctcgt aacgggtgga
     3241 ttgttcgccc aaggaacaaa ggagagtggc ggcatggtct ctaccgacgg ctccacctcc
     3301 atggaaaagg tcatcggagc gttgggcgag cagttcatgg ctgacaaccc cggggttacc
     3361 ttcacctaca accccaccgg atccggcagt ggaattaccg ccgtatcgga aggacgctgt
     3421 gacatcggac tttccagccg cgctctcaag gatgcggaaa aggctcaggg tctgaaggaa
     3481 accgttcttg cctatgacgg catcgcgatc atcgtgaaca acgacaaccc ggtatccaat
     3541 ctctcgcttg accagatcgc gaagatgtac accggccaga tcaccaactg gaaagaggtc
     3601 ggcggcaaag acgcgcccat cgttctgatc ggacgtgaag ccggcagcgg tacccgtgat
     3661 ggatttgaaa gcatcacgaa gaccaaggac aagaacaagt accgacaaga gctcacttcg
     3721 accggggatg tgatcgcgac cgttggacgg aacacggatg ccatcggata tgcttcactg
     3781 gcctccgtca aggccacggt caaggcggtc accgtaggtg gagtggttcc ttccgaaaag
     3841 acggttaaag atggaagcta cgtggttcag cgtcccttca tgctggttac caaaacgggc
     3901 acacctcttt ccgccaccgc ccagaagttc tttgactaca tcacctcggc caaagcttct
     3961 tcgatcatct cagcttccgg tgtggtaagc gccaactgat gtgaagcatc caacaaagcg
     4021 gtgctttcga tagcggaagc accgcttatt attgtaaagt tgaataagca tgatgaaaca
     4081 gagtaaaagt ctaaagcgaa ccgtcgagag agtgatacag accatctttc ttctgctcgg
     4141 gcttgcaact gtagggagcg tgctccttat cacagtgtat atcatcatct cgggcatccc
     4201 tgccatccgg gagatcgggc ttgtggattt cctcttcgga aagaagtggg cgagcacggc
     4261 gaccccgcct tcctatggga ttctgccctt catcctgacc tcggtctacg gcaccggagg
     4321 cgcgatcctg ctgggagtgc cggtgggctt cctgacggcg gtatatctgt cgaaggtcgc
     4381 accgaagcgg gtcaaggaga tcgtatcgga agcggtaagc cttcttgccg gcatcccatc
     4441 cgtcgtctac ggtcttgtcg gcatgctcgt cctcgttccc gccatcagga gtgtgttcgg
     4501 agtaccggac ggggcaagcc tgctggccgc catcatcgtg ctttcggtga tgatcctgcc
     4561 ttccatcgtg aaggtttcca taaccgcatt ggatgcggtt cccgaggaat atgaacaggc
     4621 gtcacttgcc ctcggcgcaa cgaagacgga gacgtggttc cgcgtttccg tacccgcggc
     4681 gaagagcggc atcgcggcgg cgatcgtctt gggtgtcgga cgggcgatcg gggaggcgat
     4741 ggcggtcatg atggtatccg gaaacgtgcc gaacatgccg gaattgtttc agagcgtccg
     4801 attcctcacc acggcggtag caagtgaaat gtcctattcc tccggcttgc agcgtcaggc
     4861 gctcttctcc atcgccttgg tgctgtatct cttcatcctg ttgataaacg ccgttttcaa
     4921 catgctgctc aagggaggaa agaagcaatg agccggaaga ccccaacctt gtccaaaagg
     4981 cgcaaagcgt atggcatcgt catgcagact ctcatgttgg gcgctacgtg gttcaccgtc
     5041 gcattggtgg tcttcctcgt cctctatgtc atgatcggag gccttcccca catcacctgg
     5101 gagttcctct cgactgcgcc cagctatttg acggagcgca tcggtattct tccggatatc
     5161 ctcaacacgg tgtacatcgt gctggcgacc ctcatcttcg tgcttccgct gggcgtagga
     5221 gccgcgatct acctgaccga atatgcgacc aacagaaggg tggtcgtcct gatcgaatat
     5281 gcggcggaaa cgctttccgg catcccctcc attatatacg gtcttgtggg catgctgttc
     5341 ttctgccagt ttctgaatat gcggacctcg cttatggcgg gtgcgctgac gcttgtgatc
     5401 atgaacctgc ccacgatcat gcggaccacc caggaaagcc tgaaagccgt gcctcaaagc
     5461 tatcgggagg gagcgttcgg ccttggcgcg ggaaagtggc gggtaatccg gaccgtcgtg
     5521 ttgcccggct gcatagacgg catcattacg ggcagcatcc tttccgtggg acggattctg
     5581 ggtgaatccg ccgcactgct gttcacggcc ggcttcgctc acgcggtgaa cggattgttc
     5641 aaaggtcttt ccagcgccgg tgcaaccctt accgtagccc tgtacgtata tgccaaggag
     5701 cagggcgagt tcgatgtggc attcgggatc gccgcgattc tcatgttgct gaccctcatc
     5761 atcaacctca gtgctacggt agtgggcaaa tattttcaaa gaaggaagaa ctcatgaagt
     5821 caatcatcac agtgaaggac ctgaatctct ggtactctca agtacaggcg cttaagtccg
     5881 ttaccatgga tatccctgaa aagagcatca cggcgttgat cggtccctcc ggctgcggca
     5941 aatccacatt cctgaaaacc ctcaatcgca tgaacgatct catcgacgga gtgaagataa
     6001 ccggtgaagt acgctatcgg gacatggaca tctacgcgcc ttccaccgac gtgaacgaac
     6061 tgaggcgtga gatcggcatg gtgttccaaa agcccaatcc tttccccatg agcatctacg
     6121 acaatgtagc ctacgggccg cgtacccatg agatcagaaa ccgggcaaag cttgatgaca
     6181 tcgtggagcg agccctgacc gatgcggcat tatgggacga ggtgaaggat cggctgaaaa
     6241 agtcggcgat gggtctttcc ggcggacagc agcaaaggct ttgcatcgcc agggcgttgg
     6301 cggtagagcc gcaggtgctt ctgatggacg aaccgacaag cgccctcgat ccgatctcca
     6361 catcaaagat agaagacctc gccacaacac ttaaaagccg atatacgatc atcatcgtga
     6421 cgcacaatat gcatcaagcc gtccgaatct cggatcatac agctttcttt ctgcttggtg
     6481 atcttataga atttggcaat acggagaaga tgttcagcaa tccgcaggat aagcgtacag
     6541 aggactatat cacggggagg ttcgggtaat gagaaacaaa tttgacgagc aactcgccct
     6601 tctaaatcga gagctcatcg aaatgggtgc actgtgtgag gaggtcatct ccatggcggc
     6661 cgatgcgttg ctctcgggtg ataccgagct tgcccaaaag gttaagccac tgggtggtgt
     6721 aatagatcaa aaggaacgca cgatcgaggc gatgtgcctg aaactgcttt tacagcaaca
     6781 gcccgttgca cgagatctga ggcaaatctc cgcagccctc aaaatgatca ccgatatgga
     6841 acgcatcggt gaccaagccg aggatattgc cgagatcatc ggctttttga atggacgaac
     6901 cggcagagac tgcgcgccca tcggtgagat ggcgacagcc accatcaaga tggtgacgga
     6961 gagcgtcgat gccttcgtcc agcgtgacat ggagcttgca aaagcggttg tagaatatga
     7021 cgatgtcgtg gacgcactct ttgacaaggt gaaaacctcg ctgattggaa tgattgcaaa
     7081 gcacccgcag gatggcgaat atgcccttga tctgctgatg atagccaaat actttgagcg
     7141 gattggagac catgcgacaa atatcgccga atgggtcttg ttttccgtaa ctggtgtgca
     7201 caaccgggag aatattacat gatttactgc gtagaggatg atgcgagtat ccgggaaatt
     7261 gaactgtata cattacgttc caccggattt aacgcggaag gctttgcaga cagcaaggcg
     7321 ttcttcgagg cgatcgataa ggaactgccg aaactcatca tccttgacgt gatgctcccc
     7381 aacatggacg gcgtggaaat tctgacagcg ctcaaagcag atccgagaac gaaggagatc
     7441 cccgtcatca tggcgacggc caagggttcg gaatacgaca aaattcaaag cctcgacctc
     7501 ggtgcggatg attatcttgt gaaacccttc ggcatgatgg agatggtatc ccgtgtaaag
     7561 gctgttttgc ggcgcagcga accaaaagag agtgccaaaa tcatttcaaa agacaacctt
     7621 acggtcaacc tgaccgagca caccgtacat gtggacggtt cccgcgtcga gctcacgctg
     7681 aaggaattcg agctgctccg actgtttctc gaaaacccgg gcagagcgtt cacccgggat
     7741 cagattcttt cagatatctg gggcatagac tacgatggag aaacccgcac gattgatgtg
     7801 catgtccgta ctctaaggaa gaagttgggc aatgcaagca aagccatcga aaccgtccga
     7861 ggcgttggat accgtttgga ggcacatcca tgaagaagca catattccgc tccatcctca
     7921 tcgttgcggt atcagtgctt gtattatgct ctgccgtaat gatgagcatc atgtaccgct
     7981 ccctcgtcga agaacagcgc gagcagctta aaaacaagct tgctatcgtc gccaatggtg
     8041 ttgaagaggg cggcatcgat tggctcgaaa acctcactgt gaaaggttac aggatgacgc
     8101 tggtctcatc cgaaggtgtg gttttatttg acagcgaggc agattccgct accatggaaa
     8161 atcacttgga acgcaaagaa atagccgatg cgcttcaaag cggcagcggt gaaagtgagc
     8221 gcatctcctc taccatgatt gagaaaacgt tctatcaggc gaagcggctt gaaaacggaa
     8281 acattttgcg tatttccttt acaagaaaca gtattctttc ggttcttttg cgcatgttga
     8341 cgcccgttct tgtcattctc gttgccgcca tcatgctttc agcattcctg gctaaccgca
     8401 tcgccaagcg cacgatcact tccttgaaca ctcttgacct tgaacatccg ctgaacaaca
     8461 acacctacga cgagctttcc cctttgcttc tgcgcattgc gcatcaacaa cgacagattg
     8521 aaagccagcg aagcgaactc aacaaaaaac gcgacgaatt ttccctgatc acccaaagca
     8581 tgaacgaagg tcttgtgctg ttgaacgata aaggtgtcat cgtctccatc aatcccgcag
     8641 cccggcatgt gtttcgaacg gatgcctcca gcatcgggaa ggatttcttc gtcgtggaac
     8701 gcagcagcga gatccgcggt gtcatgaaaa cggctgcaga cagcggacat ggacaggtga
     8761 agctccgtcg agccggaagg cggtacgaga tcaacgcaag ccgcatcgat ttcaacaacg
     8821 aagcggtggg aacggcgata ctcgttttcg atgtcacgga aaaagctctt ctggagcagc
     8881 agcgccgcga gtttaccgcc aatgtatccc atgagctgaa aacaccgctc cagtccatca
     8941 tgggaagggc ggagttgatt gaaaacgggc ttgcaaaacc gggggacata tcccacttcg
     9001 tgaactgcat ccgcacggaa gcagcgcggc ttttggtcct catcgaagat attatccacc
     9061 tgtcgcagct tgacgagggt ggcgaactcc catgggagaa tgtcgatctc taccagatcg
     9121 ccaaagaaac gcttgatgta ctgcaagatg cagcaaccga taaaaaagta cgattgttgc
     9181 ttaccggcac ccctgtcagt ctgaacgggg tccgcgggct ccttggcgaa atcatctata
     9241 acctttgcga caacgcgatc aagtataata acgagggtgg tcttgtggag gtttccgtgt
     9301 cgggggacga aaacaaggca attatccgag tgaaggacac cggcatcgga attccaaccg
     9361 agcatcaaaa acgggtgttt gaacggttct accgggttga caagagccat tccaaggaaa
     9421 caggcggaac cgggctggga ctttccattg taaagcatgc aaccctgctg catagcggaa
     9481 agatcgaaat gaacagcaag gtcggacagg ggacggtcat cacggtgacg ctgccccaat
     9541 gatcaacaat agcgaatgaa gcgattgtcc ggtctccctt tataaaaaca acccccgctt
     9601 tcagtaagaa ggcggggttc gtgtctttgc atattatcct caggccggcg cctttccctt
     9661 ttccaacgca taccggagaa acttgaagag cgggaatccc tccaacgcat gctcgacttc
     9721 cagaatatat ttcttcgtat catactcgac tcggcggaat tggactcgta cttttccatc
     9781 gatgcattca atgattgcat aactcggacg agtatcgcca tccagactga atccaaccgc
     9841 accggggttt acaaagcgaa ccccgtcaac ggtaaaatct gcagggatat gagtatggcc
     9901 tgaaagcacc agctggatgc cttcctcttt catctttttt gcaagaatcg gggtgttttc
     9961 atccgtttca tacagtccgt cgctgatgct tatcggagtc ccatgacaag cgagaatatt
    10021 cacatcgcaa atctcaagct gtttggtcag atcgaaggcg gcaacctcgt ctcgggcctt
    10081 ttttctcatc cgaatcgacg tgtacttgta atgtttcaag agttgctcat cctcctgatt
    10141 gttcacgaca aatgagggaa tcttttcaag atattcatca ctgttgccct tgatggtaac
    10201 gatcggctta tacttcatca gaagctcgta acacatttga ggatcgagac cgttatagac
    10261 aaggtccccg agaaacacga tatcttcggc tccggcctgt gctgcatcgg caagcaccgc
    10321 ttccaaggcg cgcaggtttc cgtggatgtc agcaaggact gctaacttca ttattatttt
    10381 acctccaatt tactccgttg catgtactgt accacaagcg attgttcaga gaaactctcc
    10441 aaccgttgat aattggcaat ttttactctt gaatctcttg cagaacgagc gttcaccggt
    10501 gttatatcat aagcgctgtc agtattgaat gcatccggcg gctattcggg ccgagtatgt
    10561 tccggatctt ccaacaatga aagaatcgtc cgcttttgag caagcgcaac ggtttgaagc
    10621 gtacgctgac tcacatgggt gtacagatcg cttaattgcc ggctggagtg tccgatcgtc
    10681 aagcgaagga tgtgttcatc aacatggccg cggagcaggg tattggccat gtggcggagg
    10741 gagtggaacg taatgttccg ttccttgagc tcttcggcgg taaagatctc gcttctgatc
    10801 aattcttctt taaaacggga gctgaaataa tggctggata caaagccgcc gccccgtttc
    10861 gaccaaaaga ccaagtcatg atcaccctca tacggattgc tctccgccaa ggcatgaaga
    10921 tcttcgatca gatgggaagg gcaggggaca agacgaattc gttttccctt tggcatcttc
    10981 aacccggcat gatcggcata tgcctgttca acggtgattg aatccttacc caatgccgat
    11041 cgcttaagcc ctctgagctc cccgctgcgc atcccggtca gcagggaaag aagaatggca
    11101 agatagatcc gtttttcact gtgttccttt gcgtatgcca tgaagcgaac caactcatct
    11161 tccgtcagaa tgccgcgttg tttatggacc acataaagcg gttcagctgc taccgttttg
    11221 gtccgatcga gaagccctcg tctttgagct tcgccaaggg cgacgccgac ggcgctcatg
    11281 atgacattga ttgtgctata acctacgttc ttctcctcga ccagagaaag ttggattcgt
    11341 tcaacgtcgt caagcgttac ttcggatagt aacaatttcg catcgatctt cggcaacaca
    11401 tgattgttca tcatattatt tcggatatga atataatctt tgctgagcga accgggctgt
    11461 aaaaggtttc ttcgcttcac gtattcgctt ttcgaccaat cataaaagct ttccagaaat
    11521 tcataaaagg tcagctttga cggttttgaa aagaccaatc cctgctgtag ggcccgttga
    11581 cagatccgaa tggcttcatc acgtctggta acaggattcc catctaattc acccaattcc
    11641 ttttttaaaa cttctacact cttcttattc ccgcgcttac cggtagcagg atcgcgaaag
    11701 agggcataat agaagaagcg ttcattgcct ttaatccggc agagcgtaaa tggtgaaatg
    11761 gccatagggc aactcctgaa atacacaaaa tgttcagatt cctgttcaat ctaatcattt
    11821 ttgcgatata ttgatatata caatcatatc tacaattcat attattacat atagttataa
    11881 tgcatcctaa ccttctttgc aagttttttc tacttactag gaaccagtaa gtagatattt
    11941 tttaatcgtg tttgtatata ttttacatat ccattctata tgctgtatat atacattaag
    12001 tgtttatata ataacattgt tgaaaattcg gatttgtaga taaatgtata tttttattta
    12061 ttatatatta aatatttatt tatttctcaa cagatattat tgagaaatat atttttttct
    12121 aatccattat atagttatta tataaacata catatattgt attatacaat ttatatactt
    12181 attatttatt atattattta tatttatatg ttatttgtaa tttataattt taccgattat
    12241 gagaggcgca caaacttccc ccatcgatta ttcattgagg aagggtggag gattattgaa
    12301 aatgctctag aaattcagtt gtttcaactc acgcttggca tcacgttcca tatcgcgtgc
    12361 cttgatcgta ttgcgcttgt cgtgcagctg tttgccgcga cagagtgaaa cctgcacctt
    12421 cacgagattg cctcgaaggt agatcgctgt tgggaccaag gtgaagcctt tttcatcgac
    12481 tttccgcctc agttgcctga tctgtctttt atgagccaga agccggcggg ttcgatttgg
    12541 ttcgtggttg tgaatatttc catgggtgta gggttgaatc gtcaaaccga tgagttccag
    12601 atttccgtct ttgattgccg catagctgtc tccaaagctg aaccgaccgt cccggatcga
    12661 tttcacttcc gtaccggtga gggagatacc gcactcaagg tcttcaacca cctcatagtt
    12721 gaagtatgcc ttgcggttct tttgcagcat cttgatgtga tccttggagt tcttcatcac
    12781 tcaaaccatc cgattcggaa cccgatgtaa tcatgtacgc tatccggttc gatcacgccc
    12841 accgaatcga tcgtgacgtc tttgctgatc atactgccgc ccttgacgat cacctgcttc
    12901 tcaagaccat aggtctcggc aagctcctgc aggcgatcat agccggcatt tctcccattc
    12961 ggaatgtagt ggctgtccgt catctcccag actcctccaa gaagaccgag gaggggaacg
    13021 gagcgctcgg aaagagcgag cgacgtatca tactcggtat gtttctggct gtatgccgcc
    13081 tgactccaca tcgcttcagt tggaaggaac acctccttgc cgcttttttc actgagccat
    13141 tggacaaacg cccgggccgc atgatagctg acattgtaga tcggccggtt ggttgaaaac
    13201 tgtggtgaca gggagaggcc ggcgaggtag gaagaactgt tcatcgcgct tttggtccat
    13261 tggggattct cctgaacgaa gagcgcccat tcatactggg ataccgggcg cttggccaaa
    13321 acgaaatcct tggtagagac aggtgcataa ccgctttgcg gtgtgattcc gtcccccatg
    13381 atgaaggagg aaccggtata ggagtaaccc tcaatcgtaa ggttgcccgc atcgagggta
    13441 atcggttttc cgaccggcgg gagctcttga ataccggctt ggatcggagc ggatttggtt
    13501 tctacaggaa agaaccgttc cgcatcactc ttcatcgtat cgttgctgat gaagctcagg
    13561 gcgagatcga tgattggttg gctattcctt agttgcaggg cttccaagtc attgtggaga
    13621 ttggcataga ccggttgata gggaaattgg ggaggatatt ccannnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnntcattgt ggagattggc atagaccggt tgatagggaa
    13801 attggggagg atattccaac gacttgctga tctcctgaac ttcattgagg agaaaggaaa
    13861 cgatcgaagc tgtttcttct tcggtcaggg tgaccggtgg cgggggaatt acccgtgttc
    13921 ggtgaatcag atttgcaaga aaaacgggat ggtcaacgtg tatctccgtt tcggcataga
    13981 tctgatcggc tttgacgtag gctatcgtat gattgccgct gggaacgaag tattggtgag
    14041 tcgccgaccc caaatacttc ccatccagca ggattcccgt ttcacttaac ggcgcctcaa
    14101 acgtcacgta tcggccgcct ttgcggattc cgggaagaaa tcccaaaaga aagatcaaga
    14161 gcagcacgac gagtatcatg agaccgagga gataatatcc gggtctcaga ttgaataatt
    14221 tcgggagaac cacctcttcc accggaggga gatcgatctt ttgtttcata cacaagtaag
    14281 gtagccatcc ctcattgcct tgtcaaccaa tacccgttgt gataccttgc ctcatatgga
    14341 ccgattctta acccgtctgg agagtttgac cgttcgatat cttacccatc ggaaagcggt
    14401 tcgcctctat cagaagatga acaagccgaa gcgcaccttc ctcggcgagc tgaaagggtg
    14461 ggcggatgcg ttggtctttg ccgtctttgc ggtattgctg atcaaccagt atctgtttca
    14521 gctcttcgtg attcctacgc cttcgatggt ttcaacgctg gatatcggtg accgggtgtt
    14581 cgtctcgaag acgatttacg gagtcgagct gtatcccacc ggaccgaaga tcggttcaaa
    14641 gaatcggcaa gtcagacgcg atgcgatcat cacgttctat aaccccgaat acgattcaaa
    14701 aggacctctg tttgacattc tttcgcagat catcttcagc ggaacgcttt cgctggtgaa
    14761 catcgaccgc aacgaggacg gttcgatcgc cgagcggctc ttcgtcaaac gggcggtcgg
    14821 cttgccggag gagctcgttc gctttgttga cgggaatgcg gagcttcgaa aagcagggtt
    14881 cacttcgttt gaggatgagg cctcattccg aaaggatctt gatttggtat ccgctcccca
    14941 tcgctcgatc gaccccgctc tgtacagcgg attgaatgca tggggatcgt tgttcggata
    15001 tcaggaagaa ggacttgatg taaacaacgc accaacccat ttgaagagca gctacaacag
    15061 tgtggcgcat ctctcctttc cccccgacta ttacaccttt gaaaaagccc gatctgaaac
    15121 tcggcacctg cttgatccga caaacctcgg gttgcgttcc gaagccagcc actatcgccg
    15181 cggcacctac gttcccaaaa acgcgattct tcctttgggc gacaaccgtg acgacagccg
    15241 cgacgggcgc tacttcggcc cggtcgacca gagcacgatc aacggcagcg tcgtcttccg
    15301 cttctggccg ccaaatcgag tacggccgat gggcgacgcc tgatgggctc cctgtacatc
    15361 catattcctt tttgcacgag ccgatgtggt tactgcacct tctattcgga actctatcat
    15421 acgcacgctt ccctggtcga accgtatttc aaacgcctgc ttgaagagct ttcctttctc
    15481 gtacatgaaa aacgattatc gtttgacacg gtgtatatcg gtgggggaaa tccgggtctt
    15541 ctcggtgtcg aacgtctgtt ttcattggtg aagctcatcc acacccgcgg aaagccgcgg
    15601 gaagtgacga tggagatcaa tcccgagaca ttgaccgaag agttctttcc tttgtttgat
    15661 gaccggctga tcacccgcct ttcgatcggc atccaatcga tgaacgacgg attcctgaag
    15721 cgaatcggac gcaacgcttc atctttgatc aatgagcggg ctcttcggct cctggaacgg
    15781 atccgcactc tgtatgatgt tgaagtctcc gtggatctga tcgtcgccat ccccggtcag
    15841 acgatcggcg accttcaaag cgatctgaag cggatcttga gcctttgcgc agccaaccac
    15901 ataagcttgt attgtctgac cgtcgaagag ggaagccgcc tctttgaaca gggcgacatc
    15961 ttcatgagcg aggatgagca agcctccctg cttactgaac tctgggcgta tctgaaaacg
    16021 ttgggctttg aacactatga ggtttcgaac ttcgcccgtg agggagcata cagccggcac
    16081 aacctcgtct attggaagct tggcaggtat atcggcctcg gtagcagtgc agcttcaacg
    16141 attgaggaag aaggtgcact ggtgcattac gcccaagagc aggatcttca agcatatgct
    16201 caaggtgaac ccttcaccgg atatgaaaaa gaacttctta ccccggttga acggatcgaa
    16261 gaggaaatca tggtgggcct gaggacccga tggggaatcg acaaggattc attttcaagg
    16321 cgaaccggat acgatttttc caccctcttt gaccggatca ttgccacctt ggatcccgca
    16381 tggtatactg aatccagcca attctttgta ttgacagaaa atggctttat ggttcttgat
    16441 gcaattctcc tgcggttttt cgaggcaatc gcagaaggcc ttgaccggcc ctagtggttg
    16501 tggtaggttt tgagcacaag aaatcttata ccatcaaagg ggccccctga tgtccggaca
    16561 tagtaaatgg gcgaccatta aacgcaagaa aggagctgct gacgccgccc gcggccagaa
    16621 gttcacaaaa ctgatcaaag agatctccgt cgctgccaaa aacggaggtc ccgatcctga
    16681 ttccaatgcg gcgctcagaa ccgccatttt aaaagcacgc tccgagaaca tgccgaagga
    16741 caatatcgat cgggcgatta agaagggcag cggtgaattg gagaattcgg tcttctacga
    16801 actcacttac gagggatacg ctcccggcgg ggttgcgatc atcatcgata cccttaccga
    16861 taataaaaac cgaaccgcca gcgaggtccg ctcgaccctc accaaaggcg gaggaacgct
    16921 cggtactacc gggtccgtct cctacatgtt ccaaacaaaa ggaatgatca tctacgacga
    16981 tcagaaatat accgaagagg agatcttcga agcggccctt gaccatggag ccgatgatgt
    17041 gcttcatgaa gacgggatga tcgaggtata caccgctccc ggcgacttcg ccaacgtcct
    17101 tgaatcgctg caggcatcgg gttttgaaga agaaagcgcc gagatcgcca agatcgccga
    17161 ccagacggtc gagcttgatg atgaacgaac tcggaaagtg ctgaaaatat tggagcgttt
    17221 ggaagagctg gacgatgtcc agcaggtatt ctcgaacctc gagattcccg acggcttcga
    17281 ggatgaagaa taaggggatg cgcatcctag gaatcgatcc cggattcgcc caaacaggct
    17341 ggggggttgt cgaaacaacg cggcaacaca cccggcttgt ttcatatggg gtaatccgaa
    17401 cgacaaccaa agagaacgat tccagtcgaa tccatttcat cgccaaacat gtcggcctgt
    17461 tggctgtcga gcacggcgtt gagatctgtg gaatggaaga tatctacttt gccaaaaaca
    17521 tctccagcgc gatccccgta gcgaaggtga tcggatcgat catgcacgaa ctgaccgccc
    17581 gagagattcc gatccgcttc ttcagtcctc cggtgatcaa aagctcggtc gtcggctatg
    17641 gaggagcgga caagacccag gtgcaggaga tggtccggat tttgctttcg cttgaaaaga
    17701 ttccccgacc cgaccacgct gcggacgcct tggccgcagc cttgtgcctg caagcccatg
    17761 aaacgactcg aataaggatg aacagctgat gattaatgcc ctgataggtg agctagtctc
    17821 cgttaaagaa ggatctatga tcgttcggtc aaacaatatc gagtatgtcc tgttgatctc
    17881 caaccaaagc gcaagccgat tctcaagcct tgataaggaa gaacgcaagc tcatcaagat
    17941 tttgacaagc ttgcaggtgc gggaagactc cttgacgttg tacgggtttt atgatgatgc
    18001 ggaacgggaa gcgttcgaac agcttcaaac ggtcagtgga atagggccga agcaggcgtt
    18061 gaagattctc agtggaatca atgtccggca tctggcggaa gcattggaca gcggaaacat
    18121 caaaacgctc gccacgattc ccggcatcgg tccaaaaacc gcccaaaaga tggtccttgc
    18181 gttgcgcaat gtgctggtgc tcgatgaacc gaagatgaaa caaacgacct ctcggacggc
    18241 agtatgggcg gatgttgtaa acgcccttgt tgagatgggt tttgagcgcc gccgttcgga
    18301 agaagtcatc ggagatttgg aagtcgagtt cgccagagag atcggtgaat tgagtcgcca
    18361 aagtgctgaa gagctgttgt tccgccatgc gctgaagaac ctgagctagg agaggatatg
    18421 gatcattaca accaattgct cgaaaactcg gtagtttcag cctccttcca ggagatcgcc
    18481 gaccggcagg aaaacgtctt gaggcccaag atgctttcgg atttccaagg ccaggcacgg
    18541 ttgaaggaga acctctccat tttcattcaa gccgctctac agcggggcga agcgcttgat
    18601 catacgtttc tgatcggacc gccggggttg gggaaaacca ccttggcatc gatcatcgcc
    18661 aatgagatgg gatcggagat ccgcatgacc agcgccccgg cgttggacaa gccgaaagac
    18721 cttgccggca tcctgacgaa cgtcacggag aactcgatct tcttcatcga cgaaattcac
    18781 cgcctgaaac cggcgttgga ggagatgctg tacatcgcga tggaggattt cgagatcgat
    18841 tggatcatcg gccaaggccc ctcggcccgg acgatgcgga tcccgctccc cccgttcacc
    18901 ctcgtcggtg ctacgacgaa agcgggcagc gtctcctctc cgcttgcttc ccggttcgga
    18961 atcaccgccc acatcgaatt ttacaacgaa gaggaactgg cgaacatcat tgaccgctcc
    19021 agcaagatca tcgatgtccg aatcagcgag gacgcggttc tcaccctcgc ccgatgcagc
    19081 cggggaaccc ccagaatcgc caaccggctc cttcgccggc tgcgggactt cgcctcggtt
    19141 ctcggtgaag gaacgatcac cagcgaaatc gtccacaccg gattgaaacg gctgggaatc
    19201 gatcagcagg gactggaaaa ccaggaccgg aacattcttc gggcgatcat cgaacactac
    19261 gacggcggac cggtcggagc tgagaccctt tcaatcagcg tcggagaggc gcttgaatct
    19321 ctggaagatt tctacgaacc ctacctgatt caaaaaggat acctcaagcg aacgccccgc
    19381 ggtcgaatgg ccacgagcaa agcatacgaa ctgttgaata tcaagaagaa gcggaacacg
    19441 cccgatgacg atcaaggatt actcttttga cctcccgctt gaactgatcg cccagcggcc
    19501 aagcgaagtt cggggagaag accggctgtt ggtcgtggat cgggcaaacg ataccttttt
    19561 tgatgaccgc atctcaagct ttcccgatct cctggaggaa ggttcgatcc tcgtcctcaa
    19621 caactcgaaa gtccggaaag cccgcctcta tgcggaaagt gaaagcggcg gaatcgtgga
    19681 attcctcttt ctatccgaaa atcccgacag gacgtggaat gcgttcgtaa cgaagagcaa
    19741 acgtcagcgg gtggggaagc gctaccgctt ccaagagcac gaggcgacga tcgtggcgga
    19801 acaaagcgac ggctccaagg tggtggcgtt cgcttctccg atcgatgaag cgtttttcag
    19861 ccgattgggc cacgtgccgc ttccgccgta catcaaacgt accgacgatc ctgacgatga
    19921 aacccggtac cagacgatct atgcccaaac tgaaggttcg gtcgcggccc cgaccgccgg
    19981 tctgcacttt accgaagacc acttaaaaag aatcaatgaa cgaggcatcg agatcgcatc
    20041 cgtcacgctt catgtagggg cagggacgtt cctgccggtt cgagccgaac gcttggaaga
    20101 tcatcacatg cacaccgaat cgtatgagat caacgaagcg gtcgcctgta aagtgatgag
    20161 ggcgaagcgg gaggggcgca aagtcgtggc ggtggggacg acgagcgtcc gaactctgga
    20221 gtccgcatat gatgaacaat ccggcatgat tcgaagcggg ttcggtaaaa cggatctctt
    20281 tatccgtccc ggtttccact tccatgtcgt cgaccagctg ctcacgaact tccatacccc
    20341 cgaatcaaca ttgctggtgc tggtttccac cttcgcctca aaggggctga tggacgaggc
    20401 atatcgccac gccgtttccg agcgctatcg attcttcagc tacggggatg cgatgttcat
    20461 ccgttgaaga tcgtgttttt tatgatttcc gagatctctt cgatcgagag gagcccgtcg
    20521 attcgagtca atttcactct tggatccaaa tcttcgaaga ttcgttcata gttggctttg
    20581 acttgaatca ggaactcctg gtgttcaaac agttcggtct ccgtttttcg atgagcgatt
    20641 cttctgagac actcatcaac cggggtatcg agaaagaaga ggtattccgg atgaggaaat
    20701 tgattcaggg ctgcgaccgt ttcatagcca cactcaacgc tttgatacgc caatgaggag
    20761 tagaggtacc ggtcgctgat caccagccgt cccgcctccg cttcggaagc gatcccgtag
    20821 atcgggttga acagatgatc ttcccgatcg gcggcataga gaagggcgag ggcaaggggt
    20881 gtcgtaacca cctgcttttg caggattgac cgcaccaagc ggccgatcgg cttgtcggtc
    20941 ggctcaaaag tcgctttgca caagcgggaa ttgcgatcac atgtctcggc cagcagctgc
    21001 atctgggtcg tggtgccgga accgtcaagt ccttcaaata cgataaaatt tttcaaaatc
    21061 gtgctcatac atctatccta cgctaaaaat gcggcggggt aaatccacat tcacagggtg
    21121 tcaaagacaa gcagatacgc tatactcatc cttgaggtca agacttgtga gatttcatac
    21181 ccgggcaaag gatatcattg ccgctacgag catcctctta ctcatcacag ccatcgtcgg
    21241 ctgggtactg atatttgatg agccgaccct ttcaccggca agtctccttg gcgactatct
    21301 cctttcccgt ctttccactg aaggcgcatc gaatctttcc ttcacatcga tcgaacgaaa
    21361 cctttttgcc aatctgacga tcaacaatct ttcctatcgg cgtggaaaag acctatcaat
    21421 cacgatcgac aaaacgaatc tggagggcgg gctcttcaac ctgtttcttt ccctcttctc
    21481 ctcgaagccg ctcctctcgc tttcatttca ggaaccgaag atatacctcg gtcccaatca
    21541 caagcccggc gaagaggcga acgcagttcc ccgccttctt cgttcctggg tacgccgcct
    21601 tcaacttgaa gcgctcggaa ccaaaggttc ttttgaagcg gagattccgt tcggagagat
    21661 cgaggtcgct gatatcgatg tagcgcttca ctggaagctc agtgatgagc ttccatcctt
    21721 cacgatccaa gcccggcgcc ttgcagcgct taccccttcc ttcacgcttc aatcctcgca
    21781 tctgctggca agccttgggc atagcggacc gttcaccttc aaaggccttg atacggcgat
    21841 ggatgcgaac gatcagcgcg ttacgattgc gaacctttcg gtagaagggg aggtcgtaag
    21901 cctgaaagac cgatctctga acgcccaagc gacccttcgc caaggcctgt atcaaacggg
    21961 tgacgtgacg gttgaaaccc ccgagctctc tttcagcggt agatatcttg acgatcgatt
    22021 gccgacggtc agcctcagcg tcccttccgt gtttgtcaac acgcccgatc tccaggcgga
    22081 aggctcacat gtcgatcttc acatctacga cctgacaacg cttctgctca gaagcgaacg
    22141 aatcgatctc accgctcttg aaacggctct tacgctcttg ggcattgaag tgcatcttga
    22201 tgctcaagag aacaatctct attacgtcac ctccacgctg aacgaaggac ggatctcggc
    22261 actcgggcac acactcgccg ttcacgcgtt tgaaactctc ggacagggga tgtatcaagc
    22321 gggaacgctt gaacgggtgg agcttcaaag ctcggcgctt ggtgaagtcc ccaatctgtt
    22381 tggaatccga tttcccttgg agacatcctt cctctacgac cgttcccatg aacacattgt
    22441 cggaagagct ttctcgcaag cccttgcttc ggatttgttt acggaagcgt tcacgttctc
    22501 actttcctat gaacgcgacc gcaatgacca ctcgctggat gtcctcatcg aacagaaggc
    22561 ggatcttcgg cttcttgccc atgcggaacg gatgagtgat catctgcttg atttcagcct
    22621 caccggtcgt ttcaatcatt tctctttggg cgtcatcgag ccgctgcttg ccgagtatgc
    22681 tccattttta aacccttatt acactcaaga taccgcgctg acgggaaacc tcttttttac
    22741 cgcccgaatc gggaagggca acgtcttggg acttgacgga cggatcagtc tggacggctc
    22801 gctcagcacg gtgcggttgg gccgctttga aagctcggcg gggttcctct ttaccgcggc
    22861 agtcgtggaa gatctggtcg atgtgcgaag cgccgctctc tccgccgaag ggtatcaact
    22921 ctcctatagc ggcgagataa acctgaccga ttctttcacc cagggcagcg ccagacttct
    22981 tcagatcgat gacaaccagt tgcttgcgac gctgggcatt cgcggcagaa tgaatcacgt
    23041 cctaacactt gatttctcga ccccgctggt tcgtgagctt gcggtgggaa tggatgtcag
    23101 cctgaaggat cagcggcttg ttcacggagc gggagaggtg cacctttttg atatggacta
    23161 tcccttcacc ctcaacgtcg atctttcact tttggaattc agccttgagc aggagggggc
    23221 gctccgcatc agtggaacgt tgatgccgat catcacgata gcctttgacg cgttctattt
    23281 cacccttccc atcagccgca cctcgataag cggaaaagcc cttctttcca tcgaccatta
    23341 ctcctcattc ctctttgaaa gcgagcagat ctccattcac aaccttgcgg ttggacctac
    23401 cgtgtatagc atagatgccc agttgagtgt ttccagcgat gagctcttta ttcgcaacgc
    23461 tcggttcacc gattgggtca acacctacag cggcaacgcc cattaccaag gctcacccct
    23521 gttctctttg ctgaaacacc gctttctcca acccttttcc ttcaatctct cgatcttccg
    23581 cgatgagtcg cccatcatcc aaaccactct tatcggttct tcgaaccgac tccaatcgat
    23641 gatcacaacc aatggtcttt ccctcggctc gttcgtttcc gcccttgaag gcacctatct
    23701 ggatctcacg cttctcggtt cgagcgatct ttcttccgaa atcaacctct ccggttcagc
    23761 ggagcttcgc agcgatgcat tctctctctc gacgaaaatc ggtgccgaag gcgataactt
    23821 ccgcttatat gattcattct tccgatggaa agaacttacc ttcagcgggg atttgctttc
    23881 gatagcgaat gaggaagcac ggcttttggg atcctttgag catatccgcc acctttcgta
    23941 catcgaccag ctcagccggc tcgacgtcca ggtgggtgtt gcgctgccgg gagttcaaaa
    24001 cctcttcatg cttcccgatc gtgcaaaacc gcttcttact caaggaatcg gaacggcatg
    24061 gatcacgatt ggcgaaccgc tgatctatgg cgaaggcggc tttaccggag gaacccacgt
    24121 cttggacagt gacagtaccg cactcatcgt cgacggctca ttcctgcggg gcagatatga
    24181 ataccgaacg cgtacgcttt ccgcccggct cgatccctcc ttcggtatcg gggcgacgat
    24241 cagagccgac ctgagaccgg aatccttcgg agtgaaggtg gaggatatcg tattccccct
    24301 gccatatctg aaccgaacat acctgaagcc tgttttcgcc ttcctggaag gcaccgcaaa
    24361 cggtaccgtc ttttttaccg gatcgatgca aagcccgcga atatacggac agctgagtgt
    24421 cgactcctcc caaatgacga ccttctgggt gtatgatgat atcatttcat tgaaaaacct
    24481 aagcgtttca atcgatggaa accgggcgat cagcccccat cttcccttct tcagcaccaa
    24541 ccggaaaacc ggcaacgtca ccaccggaac cgcgctgctg cgcgctcaat tgggcggtct
    24601 tggtctcgat cattatgaga tcacgatcga atcgatcgat gatcccatat acctgtggat
    24661 tccgatgctc gggtatgatg ccgatctcaa aatgtgggcg gagggatcat tcaatctgtg
    24721 gggaaagggc ttggagacat ggctcagcgg agatttgatc atcagcgatg cggtcttaag
    24781 ccttggtgtc cggggcttgc cctattggta tacccatgaa aaaggaactt cgaacgattt
    24841 caaccttact accgacaaag gggtaacatt cctttatccc aatagcacga acccaatcat
    24901 caaagccacg atcagtgaag gggagaagct ctcctttacc tatgaccacc tcaccgatga
    24961 gattacaatg gatgggagtc tggcgctccg aagcggtgaa atctattatt tccaaaaaga
    25021 cttcttcatc accgaaggcg ctctcaccgt gcacaccgaa tccttcggag gacttccatc
    25081 cgttcaaccg acgatcaacc ttcgggcgaa gctcaccgac tatgattcaa gcggaaaccg
    25141 ggtcgacatc ttcctgattc tccgagaagc gagttttacc gcattgaacc ctcagttcga
    25201 atcaattccg gcaaaagcca ccaacgagat tttcgagatt ctgggtcaaa gcattctccc
    25261 cagcggtgca tatggacaat tcaacctcta ttcggccgcc catctcgccg ccgctgcgac
    25321 gaacgtcgcc gaacgtctcg ggtacatcaa cgttttccaa accagcgccc ttaccgaaac
    25381 gatccgagtc tcgctcggat tggatatgtt cagcctcagg tccaacattc tgcaaaatat
    25441 tctgcttgac acactccccg gggccactcc gggaacgacc ttttcacccc ttgcccggta
    25501 tttgaacaac acggtgatct tcatgggtaa atatattgga acggagtttt tcctccaggc
    25561 attgctccat ttgagcgcca ccgaaccgaa taaaatccgt aatcccttcc ttgcgcccga
    25621 cctttctttg aaccttgaac tttctatcga gtggcataac ccgttgctta ccttctcgct
    25681 cttcacccag ccaaatgagt tgtcgttcat tgatattttg gatacgatgg gcgttagtgt
    25741 aaccaaacgc atcgttttgc gttaagtgag gtaagaggac aaatatgaag agcttatatc
    25801 gtcgaattct ctggatagcg ctgctgatcc tgcttgccac tgtgaatatc tttgccagtg
    25861 atgccgagcc ttggtatatg ggccggccga tcgcttcctt tgtcaacacc ggcttgctga
    25921 acgtccggga aagcgaaatt cttgatatcc aatatgagta ccttcgcaaa ccattcagcg
    25981 accagctctt caatgaactt caatcaaaac tctatgcgtt ggagtatttc tattactttc
    26041 tggctgatgc cgagcggacc ggggagggcg gaaacgagct gagcatcgtc atgcactttg
    26101 aagagcttcc gtacgtcaac gaggttctca ttgaaggcaa cagcgggctt aaaacaaagg
    26161 atattctcgg ggcttccctt gtacaggccg gcagtttctt cgatgagtac accctcactc
    26221 tttcgagaga cctcatcgag cagctctacc gggaaaaagg atatatcaac gcacagatcg
    26281 aacggaccta caccgaagac gaggcgacca acaccgtatc gatcctcttt gagatcgaag
    26341 agggcagtca atcccgaatc ggtgaaatcg ctttcgaagg ggtgcactcc gttcctccgg
    26401 caagtcttgc caagcaactg gagagtaaaa gcgtgtcgct cttcaatccc ggatactaca
    26461 acccgctttt ggaagagaca gacaaaaaag cgatcatcca atactatcag aaccgcgggt
    26521 atatcgatgt cgaagtgggc cccgcctatg ttgaagacat ctcttccgac gatgataaat
    26581 atacccgtct caggctggtc tacccgattg ccgaaggcag tcagtggttc ttcggcggaa
    26641 tcgaggtcag cggaaatgag gtgttcagcg atgaacagtt tcagaacctc atctcgataa
    26701 aaaccggctc ggttctggat ctctcccgga ttcaggctca aatcgcggcg ctcaccgatc
    26761 tgtattggaa caacgggtac atcttcaacc tgatcagcag cgacgaggtt cggaatgagg
    26821 aaaacaagac gatcacgtat cggctccatg ttcaggagaa ccggcaggca atcatcgaag
    26881 atgtgcggat tgagggaatc acccgctcca aaccatacgt cttcgaacgg gagcttgcat
    26941 tcaagaaagg ggatatcttc tccaaagagc tgctcatcaa atccgctcaa aacatttaca
    27001 acaccctgat cgtcaccgat gtccaattcg acatcctcaa tggaacggag gaaggaacgg
    27061 tcattcccgt cttcacggtg acggaaggga accagatgga tcttcagttc ggagcgacat
    27121 tcggcggcaa cgtcgacgga ttccccgtca gcgggttttt gcagtggtca aacaagaact
    27181 tgggtggcac cggacgggac ctctccgttt caaccaacct cagccccgat acccaaacgg
    27241 tgatgatcgc cttccaggat gattggttca gatcctaccg atggtcaaac gggttcacct
    27301 tctcctttga acgaagtgtg aaagaaaata cgctcacccg aggccagggc agtggttact
    27361 tcaccacctc gaccagtgcg aacacctatc cgaacggata tgaaagctat caggcatatc
    27421 aggcggcggg ccaagcatac cccaccgctg acaagctgat ggaatatgtg caattccgct
    27481 tctccctcgg atataacacg ggatatacgt tcatgttccg tcccggtgcg ctttcgatcg
    27541 gggtgggggt gtcgatcggg ctgaacaagg cggagtacga taaggccatc tacgatccct
    27601 atgagtggct gatcaagcag tatggagaaa aatggcaatt ctccaaccgc ctctcgctca
    27661 ccttcgcctg ggacggacgg gaccggatcg aaaacacgag ccgcgggtat tatatctccg
    27721 agaccttcac ctatgccgga gggatcctct tcggtctttc caactatatg cgaaccacga
    27781 ccagcctcag cgggtatctc accctcttca gcttccagct tgaggagaaa ccggcgaacg
    27841 tggtactcgg catgacgacc acggtcgggg cgatgctgcc gcagttccat aatcccgtaa
    27901 cccaagacgg cgagcagggg tggggctggt atgacgcgaa gatgggagcg acccggtatg
    27961 agatgctcta cctcgacggg atgaataccg cccgaggatt cccggtcgtc tttgaccagg
    28021 cattcctttg ggatacccag ctctccgtct cctggccgct cgtgcacaac gtgctttccg
    28081 ccgaactcta catctcggca agcggggtaa gccaaagcct caaggaaacg aagatggatc
    28141 agcttgcctg gtacttctcc gccggcggcg gaatcaagct caaggttccc gggttcccgc
    28201 tcgggctcta tctggtgaag aatgcgacct atatcgacaa caccttcgca tgggatacga
    28261 accacttcct cttcaggggg cagaacgaca ccagcggctt gaaactgatt cttgcgatca
    28321 cgacgacctt gtattgatat gagtaaaaca cagcgaaccg acactccgat gatggctcag
    28381 taccgttcga tcaaagagca gcatcccgac tcggttctct tttttcgcct cggtgatttt
    28441 tatgagatgt tcgaagacga cgcgatcgaa gtctcgcgcc tcttgaacat caccttgacc
    28501 gcccggggag gagtccccat ggccggagtt ccctaccatg cggcgaaaaa ctacatcaaa
    28561 cggctcctgg atgtcggaaa aaaagtcgcc atctgcgagc aaacccttac cggagaaaaa
    28621 cttgcccaac gggaagtcgt tcaggttgta accccggcga cggtcatcga ggacgatttt
    28681 ctccaagcac acgatgcgaa cttcctgatg gctgcaacaa tccggaaggg gtcgatcgtc
    28741 accagctacg ccgacttggg ttcgggaagg ttcttcgtcc gccaagtgga gcacgatccc
    28801 gggtatatcc acctgcttgc ccttctagaa gaggttcgtc cccgtgaact gctggtcaat
    28861 gaagatgagt actttctgct gggggagttc aaggaggcga tcgatcaaac atcggcgatg
    28921 gttacgaagc ttcctcccta tcaattctcg gcaaagaagg cgtatgagag tttgctggaa
    28981 cactttgagg tccatagcct caaagcgttc ggcatcgatg accatgctcc ctatctttcg
    29041 agcgccggtg cactcttatc ctacctccag cagaccgccc gctcctccct caaacagatt
    29101 gaaactctca ccttggtcca ttccgccgaa ttcttgcaga ttgacgagga ttccagaaaa
    29161 tcgctcgagc tttttgccaa cctcagtgac ggatcgccgc gatacacgct ctttgattcg
    29221 atcgactcga cggtcacctc gatgggaact cgaaaactca aacaatgggt cgcttcccct
    29281 ttggcaagaa aagagacgat tttttcccgc caccgctggg ttgatcttct atatcacgac
    29341 cgcgaggagc tggaacgagt tcgacacgag ctcaaacagg tcctcgatct ggaacggttg
    29401 accagcaggg tggtgatggg tcgtcacctg cccaaggaca taaccggaat cgctcaggcg
    29461 ctgaggggct ttttccttct cttcgatgaa cgctaccggg atcttcttgt caccacgcag
    29521 caggaacatg aaaaggcctt tgccttggcg acccatatcg atcgggcgct gaatccgacc
    29581 caccaaggtc cctttgagga ggggagggtg attcttgacg gatacgattc cgagctggat
    29641 gagctcagaa agatcaaagg cggaggaaaa gagctgctgg cggagtatct tgaagagatc
    29701 aaggtccaaa ccggcattcc gacggtcaag ctgggcaaca accgaattct cggccactat
    29761 cttgaagtga ccaaaatcca tagtgacaag atgcccgcaa cgttttatcg caaacagacg
    29821 ctggtgaacg ccgagcgctt caccagcgat gaactgatcg ccttggagcg aaagatccag
    29881 cacgctgcgc tggatgcgga gcagtatgag cgagccctgt atgagcatct ggttgaagaa
    29941 accctcgccg cccaaaagac cctcttggca atcgccgcgt ttttaagcga tctcgactgc
    30001 tatcaaagcc tggcctatcg ggcccatatc tcctcattcg tgaaaccgga gtttgttgac
    30061 gaggattgcc tcatcgtcga aggaggacga catcccgtcg tcgaagccaa cctgccttcc
    30121 ggcgagttcg tcgcaaatcc cctgacgatt gaaaaagaac accggaggtt ttgtttgatc
    30181 accgggccca acatggctgg aaaatcgacg tatcttcgac aaaacgccct catcgttctt
    30241 ctcgcgcaaa tcggttccta cgttcctgcg gccaaggcga cgctttccct ggtcgatcgc
    30301 ctctattgcc gggtaggagc aagcgataac cttgcccgcg gcgagtcaac gttccttgtc
    30361 gagatgctgg aggcggcgca catcctgaga accgccacat cggcatcgct tgtcatcatc
    30421 gacgagcttg gacggggaac atcgacgcag gacggcatgt cgatcgccta tgcggtgatg
    30481 agagagctca tccacatcgg gtccaaaacg ctcttcgcca cccattatca tgagcttgcc
    30541 cggaccgaag agcaaaacct tcagctgctc accttggagg tgttggaaca gggaaaggag
    30601 attcgcttcc tcaggcgggt catcgaaggg gttgcaaact cctcctatgg cttgaacgtc
    30661 gccaaaatgg cgggcattca tccgagcatc ctcaaaaacg ctcaaagctt tcagcggcag
    30721 cacttcgccg actacgatct ttcctccctg cagatgaatc tcttcagcga acgggaggat
    30781 gcgccttctt cctacaatga ggtcgaagag caggttctgg tggatctgga aagcttcgcg
    30841 atcgaacatg cgacgccggt cgacgcgctg ctcttcctga aggaactgca ggaacggttg
    30901 aaagcgaagt aggaccttcc gccccatagg aggcatgtac tgtatcggat gcgatcggat
    30961 cagcgatgaa ctcctttgtc cctcatgtga tgggaagatc caagcgggat cgtacgcata
    31021 tctctatgac cggcgctgcc ctgattgcca cctgccgatc ttcgattccg cctatccgtg
    31081 ttcttggtgt accgacgggc gaatcggctg gggagggtat gaagggctgc tcgcccgact
    31141 gcttaccctt cataaaagtc gcaaggaccg gagactggtt cactacctcg ccaagcttct
    31201 tgttccgatg atgaacatga tagcacccga tctcttcatt ccggttcccg cctcagaggg
    31261 aggctgtcgt caacggggat atgatcagat ggtgctgttc tccaaggaat taagccggca
    31321 aatgggaatc ccaagccggc cggtcttgat tcaaaagaag ggggcgatcg ttcttctcac
    31381 gaggaatatg agaatcgaga acaagcgaat cgttcttctg gatgacatct atacgacggg
    31441 aaagacgagc tccgattgca ggaagatcct caaagagacc tgggggatcg atccattcgt
    31501 ggtgacgatc gccgaatcat gaatatacga aggttctgtt gacacgtttt ggctatctta
    31561 ttatgataca gacaggattt attgattatt gggggatcgc cgaaaggtgc atcccctttt
    31621 tatggagcaa caaaacgcgc atgctaagca aagggattca ggaaacgccg ctgtttgtca
    31681 cctttgatcc gttgatcaaa gcggtcaatc tccgcttggt cgacctttcg gtctttgaaa
    31741 gtcgggggca cgtcggggtg aatctggtga ttatggctga aaaccgcaat accacggtgg
    31801 aggactgtgc aacggttcac aagatcgtcg caccgagact gaacgttctc tacgacacca
    31861 gagaggtggg tatcgaggtt tccacccccg gtttgcagcg caatctgaag gatgtgtacg
    31921 agttcacgct ctatagcgga aaacgggtgc ggatctattc aacgatcctc aagggatgga
    31981 taagcgggcg gattgagtcg gtccaagaga actccctgac cctcaacgat gtattcgttg
    32041 aagatacaaa agaacaattg gaacgcaaat cggtcgagtt tgatgagatt caaaaagcga
    32101 aactcgatta ccgatgggag gatagaccac atggcaactg atattggtga tgcagtacga
    32161 gcgatggtcg cagaaaaaaa catcagtgaa gagctggtga tttccacgat tgaagatatt
    32221 cttcgtgctg cattcaagcg aaaattcggt accgatgaga atgcggtgat tcaattcagc
    32281 gatgattttt ccaccgttga gctggccagc cgccgcctcg tcgtcgatga tgacaattgg
    32341 tataacgaag ttacggaaat cccatattcc gaggcggttg gtctgcacga aggagcgaca
    32401 atcggcgacg agctcctgat tccggtcgat ttgaaagact ttgaccggat cagcgtccag
    32461 tcggcgaagc agcgcgccca tcaaagcttt cgcgatatcc aaaaagacac cctgtacagc
    32521 gaatacaaag cgaaagaggg aatggtgatc atcggatact tccgccgcca actccccaat
    32581 ggagacatct acgtcgatat cggcaatacg gaaggtctcc ttcccaagcg gaaccaatcg
    32641 agtcgggaaa gctatcaaag cggcgataag atcaagtgct acgtggaaaa agttgaaaag
    32701 gcggaacggg gcttgagggt catcctctcc cgatccagtc ccgagctgct gcgccggctc
    32761 tttgaagtgg aagttcccga aatcgcccag aatcaggtcg acatcataaa aatcgttcgg
    32821 gaagccggtt atcggtcaaa agtcgcagtg gcctcccgca acgatgagat cgatcccgtc
    32881 ggtgcgtgcg tcgggttgaa gggaaatcgg attcagacta tcatgagcga gatagaaggg
    32941 gaaaagatcg acatcctgcg gtatgatccc aatccggtca attacatccg caatgcgctc
    33001 tccccggccc aagtccgtga tgtcgtgatc attgacaaaa acatctatca cgctgtggcc
    33061 atcgtcgatg agagccagct ctctctggct atcggcaaac aaggcttgaa tgtccgcctc
    33121 gccaacaagc tggtagactg gatgatcgac gtgaagaccc aagagcagtt cgaagagatg
    33181 gatatcgcca aagaagctcg ctatcgagcg gaatcgatct tcatcgacga agacggccaa
    33241 cagtattaca gcgacgagca gcctgttcac ttcgaagaac cgatgtttgc cgaagaggag
    33301 aggatggatc ttggaatcga tgaagacgag atcgccctta ctgaattgcc gctcggggaa
    33361 gagttgatca cgaagttgca cttctacgat atttataccg ttgaagagtt catcaatatc
    33421 agtgatgaac aactggcgac actggagaat ctcactgctg aagagttgat tcgcattaag
    33481 gataccatca ccgaacacgt tgatatcgtc gaagatgatg atgaagagat gcagtacatt
    33541 tgtccttcct gtggtggcat catcactgag gatatggata tatgccccca ctgcggcacc
    33601 ggtttggcgt ttgaggaaga gtaactcata gaggaaagaa tgacggaaga gaagaaaaag
    33661 cctaaaatca ccctgatcaa gcaagtagtc gcccccaacg aacccgttga ggagacgaaa
    33721 agcacctcgg ataaggaagg cgaacaaaaa cccaagacca ccgaaaaacg ccgagtcgtg
    33781 gtggtgaaga aaaaagtagt gaaggcgaag cccgctccca agccggtgcc aagcaagagc
    33841 gaaccgcgga aaaagcagga tgagaagagc gcggctcctc cggctgctcg caccatcgtc
    33901 cgcaaagagc ccgtcgtctc cgagcggctg ggttcctcgc cgctccatac cggtccgaaa
    33961 gtgattcgtc cgacgaacct tccgccggta cccaaccaag gccttacgat caatgacact
    34021 ccccagcggg ttcaacgtcc gccccagcca cagcagggtc gacgggtgta tggaaccgtc
    34081 ggaggtcgac cggctcgaca gcaggaggga tcaagagcgc cccggcaagg tggtggacaa
    34141 cgatcttctc aacgccctcc ctaccaaggt ggcggacagc gccaagacgg gtatgtccag
    34201 cgaccgcctc agggtggcgg tttcactccg cgccaaggtg gccgaccggg cgggttcaga
    34261 ccttcgccga tgggagagga gctcccgcca caggatcatc agcgcccgca gaagaaaacc
    34321 ttcacaaaac gaaaggatca gacctacaag cgccgcgatg ctgaagaaga gcgcgagttc
    34381 cagattcagc ggcggaagga acaagccgcc gccaagctgg ctgccgttcc caaatccatc
    34441 gatatgatgg aggtgatcac cgttgcggat cttgccaaga agatgaacct gaaagccgcg
    34501 gaaatcatcg cgatgctctt caagatggga atgatggtaa cgatcaacca gcaaatcgac
    34561 tttgaaaccg cacagatcat ctgtaatgaa tacaactgtg atgtccgatt ggtctccctc
    34621 tacgacgaga cgatcatcgc cagcgatgag gatgagaatg aggaacttgt cgagcgggct
    34681 ccgatcgtca ccgtcatggg acacgtcgac catggaaaaa cgaagctgct tgatgcgatt
    34741 cgttcgacaa aggtggccga aggggagtac ggcggcatca cccagcatat cggtgcgtat
    34801 aaagtaaatc tgccggggaa aggagatgtg gtgttcctgg atacccccgg gcactccgca
    34861 ttcagcatga tgcgggcccg cggcgctcaa ctgaccgaca tcgtcattct ggtcgttgcg
    34921 gccaatgacg gcgtcatgcc ccaaacccgc gaggcgatcg accacgccaa ggcggccaac
    34981 gtcccgatcg ttgtcgccat caacaagatc gatcttcccg atgccaagcc cgaacgggtg
    35041 atgcagcagc tctccgacct cggcctgatg cccgaggagt ggggcggaca gaccctcttc
    35101 tgccagattt ccgctcttca gaaactcgga atcgaggaac tgctcgatac cgtgcttctc
    35161 caagcggaga tgctggaact gaaagcggcg gtgaactgcc gcgccgaagg caaggtgctt
    35221 gaatcacggg tcgaccaagg tcggggtatc gtcgccagtg tcctcattga acggggaacc
    35281 cttcgtcagg gagactatta cgttgcgggc atctatgccg gccgcgtccg ggcgatgttt
    35341 gacgataccg gaaacaagat cgaagaagcc ggtccctcca cccctgtgga gatcatcggt
    35401 ctctccgata ttcccggagc gggcgatccg ttccaagtca ccgaggatga gcgccaagct
    35461 cgccaagtcg gcgccaagcg gcaagagctg gagcgcttgg gtgaaagcag aaacgtgaag
    35521 aaggtcaccc ttgaatcact ctattcgaag attcaagagg gtgaaattca agacttcaac
    35581 gtaatcatca agggagacgt gcaaggctcg gttgaagcgc ttcaacaatc cttggagaag
    35641 ctttccaaca atgagattcg gctcaacgtc attcgagcca gcgccggagc gatcattgaa
    35701 agcgatgtga ccttagccag cgcatccgat gcgttggtca tcggcttcaa cgttcgaccg
    35761 actccccgtg cccaagcgct tgccgaccag gagaagattg aaatccggaa atacaacatc
    35821 atttatgatg cgatcgatga catccgcagc gcgatggaag ggatgttggc tcccgagctt
    35881 cgagagattg agatcggtac ggtcgaggtt cgcgaaacct tcaaagtgcc gaaggtcggc
    35941 ctgatcgcag gttgcatggt caccaaggga aaggtcaagc gaaacagcta tgtaaaggtg
    36001 attcgagaca gtgtccagat cagccccgag cggcttaaaa tctcttccct gaaacgcttc
    36061 aaggatgatg cccgtgaggt catcgaaggg tttgaatgcg gtatcggtct cgaacacttc
    36121 catgatctga gagtaggcga tatcctcgaa atttatgaac acaaggaaat tgccagaaaa
    36181 ctggagctta atggacaggt atgagtgact ttgtacgaca acgccttgag gcaaagatag
    36241 cggaggcgat ctccacgttg atcatgaaag gggagatcaa aagccccgag ctctccactc
    36301 tggtttcggt aagcagagtg gagcttgccc cgggcaacgg cagcgcaacg atctacatct
    36361 ccagtgtgtt ggatgatgca tcgcttaacc agagcgtcga agccctcaga cgagccggtg
    36421 gttttatcca aaagcgcgtc ggagccttcc tcaagactcg aaacaccccg gtcttgacgt
    36481 tcaaggcgga tcactcattg gcggatggac ataagatcaa caccctgatc gactcgttga
    36541 tgaatgatga gcacggcaag tagaatcatc ctgctcaaca aagagagcgg ggtgacgagc
    36601 tttaccgccc tcaacaaggt aaaaaagacc tttggacgga aagtcggcca cgccgggacc
    36661 ctggacaagt ttgcccaggg tttattgatt gtcctcaccg gggcgatgac caagctcaac
    36721 ccgctctttt cctcgatgga caagagctat cgggcgcaca ttctctttgg cagcgaaacc
    36781 gacaccctcg atccggaggg aagggtcatc gccaccggag agatcccctc tttcgaagcc
    36841 gtcagggaag cgatacaaag ctttgatgga gagatgcttc aaacaccccc gatctactcc
    36901 gctcttcacg tcggaggcaa gcgggcgagc gctctggccc gaaaagggaa gcctgtcgaa
    36961 ttggaaagcc gaccgattcg gattgactcc ttcgaagtcg tgagctatga agatggaata
    37021 ctgatcgccg acatccgggt ttcaaaggga acctatatcc gctcgatcgc ccgcgatctg
    37081 ggccttgcag tctcctcgcg ggctcatctg gttgccttgg tccggacctc cattggtccc
    37141 tttgccctct ctgaagcgac tgaagtcgat gaacaagttg atttgacgaa cgactacctc
    37201 agccgcctcg aacagatggc gctcgccacc gtcagcgatg atcagctgtt cccccttgca
    37261 aacggtcgct atcccgatca ctatgacctt atgaaagagg gaagcgacta catcgccttt
    37321 tacagcgggg atggagtgtt gcgagcggtg ggcaacaaga cgaagcggcg cctcatcgct
    37381 caggtgtaca ggggaggggc agtgtgaaga ttcatatgtt tatggatctc agtcgaacga
    37441 agattctcaa agacgttccg atggtcgtat cggttggcgt ctttgacgga ttgcacgaag
    37501 gacacctctc catcttgagg cgagtcaaag agatcgccga ttccaacaag atggagtcaa
    37561 tggtcatcac cttcgatcgc aatccgaaga tggctgctaa atcgatcgaa catcacgatc
    37621 aattgatgac cgaccgtcaa cggaatgagc ttcttgcctc cttggggatc gatcatcttg
    37681 ccattattga cttttgcccg gaattcagta aactcacagg ggaggagttc ctcgctttga
    37741 tcagtggcct ttgccgcctc gaggcgatgg ttgtcggcga agatttccgc tgcggtgtcc
    37801 cgacgtcatc agtcggatct gttcagctgc cggaacatct gaatcgattg tcaccggggt
    37861 cctttgtgga aatccccccc ttcgtgctca cgaaggatgg agagatcacc agcagttctc
    37921 tggtgcgtaa aaaacttctt gagggcgctt tggaaggagt ccaaagtatg ctcggccggc
    37981 cttatacgtt ggatttagtc tcccataccc ctatatccac ggtaaaaggc ctgctgtacc
    38041 gtaccgaatc cttcagccag ttgttaccga aagagggcgt ctatgacgct actctcgcgt
    38101 atgccgattc ccgtgaggtc gacctggtcg ctcatgttat ggcagagggt gttcttttga
    38161 gcagcatggc tgaatcggat gcggacggta tatcatatct tagcctctac gctaagggga
    38221 gtcgtacatg attacgaaag aacagaaaga agagatcgtc gtaaaatttg gcggcgatgc
    38281 aaagaacacc gggtcgaccg aggtgcaaat cgccctcttg accgcccgaa tcaacgatct
    38341 tcaagatcac tttaaaaaca accccaagga tcatgccggt aaccgcggct tgcttaagct
    38401 ggtcggacaa cgccgccgtc ttcttaagta tctgagaaac cgcgatattg aagcgtaccg
    38461 caccctgatc gccgaactcg gcctgagaaa gtaggtgatg gggaatcgat agcaagcagc
    38521 tccggtgacg tatcaggtca ttggagctct tttgcgtcaa agaagaataa agaacgaaag
    38581 aaggaatgaa tgatggaaag taaaacagta cgtgttcgga ttggcgactc cgatctggtc
    38641 ttcgagacag gaaagatcgc aaaacaagcc aacggtgcgg tatatgcctc ctatgaagga
    38701 agcgccgttc ttgccacggt ctgcagtggc ccggttccaa atgaggagct tgattatgta
    38761 ccattaagcg ttgaatacaa tgaaaagtat tacgccgcgg gaaagattcc cggcggcttt
    38821 ctcaagcgcg agggaagacc gagggacaag gagattctcg taagtcggtt gatcgaccgc
    38881 ccgatgcgtc cgctcttcga caaggcgttc ggccgggaaa tccaagtcat tcccaccgtc
    38941 atttccagcg acatgaacag tacgcccgac atcatcgcga tcaatgccgc ttcatgtgcc
    39001 gttgtgatca gcgacatccc gttcaacggc ccgatcgccg cggtgcgggt gagccaggtc
    39061 gccgggaagc tggtcatcaa cccgacgttc agtcaaatcg aggaatccac ccttgatatc
    39121 gtcgtcgccg gcacccgtga cggcatcacg atggttgaag gcgctgcaaa ggaagtcgac
    39181 gaggatctga tgctcgaagc gatcgaagcc gccgtaccgg ttatccggtc gatcgtcgat
    39241 gcccagcttg aactggcgaa gatcgccggc aaggaaaaac ttcctctccc cgtgttcgag
    39301 aaagatcttt cattccttca gccgatcaag gcattcgccg agccgaagct caaagaagcc
    39361 tcctttgtga agggaaagat ggaacgccac gctgcaattc atcaggttct cgaggagacg
    39421 cttgataagt ttgcttctgt attggaagaa gatgccaagc gggaaaaaga agtccgagcc
    39481 ctcttcgagg agcttgagta cacgattctt cgctcctcga tcctgaacaa cggcgttcgg
    39541 accgacggcc ggaccacgac ccagatccgg aagattacct ccgaagtcgg cctgttgccc
    39601 cggacccacg gttcggcgct cttcacccga ggcgagacgc agagcctggc ggtcaccacg
    39661 cttggaacga cgagcgatga acagatgttc gacacgatcg acggcgagaa gagcttctcc
    39721 tccttcatgc tgcactacaa cttccccccg ttcagcgtag gggagaccgg tcggctttcc
    39781 accggccgcc gcgagatcgg gcacggcaac ctcgctcagc gagcgctgga aagcgtcatc
    39841 ccttctaagg aggagttccc ctatacgatc cggatcgtca gtgagatcac cgaatccaac
    39901 ggatcctcct cgatggcctc ggtgtgcggc ggaacccttt ccttgatgga tgccggtgtc
    39961 ccgatcgcca aaccggttgc cggaatcgcg atgggcctga tcaccaaaga cgccgactac
    40021 tcccaatacg ccatcctctc cgacattctc ggtgaagagg accaccttgg agacatggac
    40081 ttcaaggttg cgggaaccag agacggaatc accgcattcc agatggacat caagatcgcc
    40141 gggatcaccc ccaaggtcat ggccgaagcg cttgctcaag cgaaagacgg ccggatgcac
    40201 atcctgaatg aaatggacaa aacattgagc aatccccgaa aagagatcag cgagttcgca
    40261 cccaaaattc tgacgatgaa agtcgacgag gataaaatcg gtgcgatcat cggaaccgga
    40321 ggaaagacaa tcaagggaat cgccgcccaa agcggtgcgg aagtgaatat cgaagacgat
    40381 ggaacgatca tgatctatgg ccggaacaac acctccgccc aaaaggcgaa agagctggtc
    40441 acctcgatca tcgaagagcc ggaagtcgga aagatctacc agggaacggt gaagcggatc
    40501 atggatttcg gtgcgttcat cgagattctt cccggaaagg aagggctttg ccacatttcc
    40561 aagctttccc gctcccgtgt ccaaaacgtc gaggatgttc ttaccctcgg tcaggtcgta
    40621 ccggtgaagc tgatggagat tgaccgacag aatcgactca atctttccta tatcgatgca
    40681 attgatccga aggaatcaaa atgatcgcag tcaaggtgct tgccctcaaa gcgggggcaa
    40741 gcttgccggc atatcagacc gccggaagcg ccggagcgga cctgaaggcg cttttggatg
    40801 aaccggtggt gattgaaccc ggcaaccgtg ctctgattcc caccgggatc gccttggaac
    40861 tgccggtcgg ctatgaagct cagattcgtc cgcgcagcgg cttggcggca aaagcaggcc
    40921 tgacggtgct aaacgccccc ggaacgatcg acagcgatta ccgaggagag gttaccgtca
    40981 ttctcatcaa ccatggtagc gaggcggcga tcgtccgaaa cggggatcgg atcgcccagc
    41041 tcgtcatcgc accggttgtt caagcttcct tttcgcctgc agatgcgcta gactcgacag
    41101 agcgggggag tggagggttc ggttcgacgg gagtgtaacg aggatggcta aaaggtatgc
    41161 ccttgtctat cgccacgtat caagagagta tctgctctcg ttcttcgtgg cgttcctttt
    41221 tttcttcttc atctttttca tcaaccagat ccttctgctg gctcaaagaa ttctcatcaa
    41281 acagatcgac tattattccg tcttcaaggt gattatctat tcgattcccc agttcctcct
    41341 cttcaccttt cccttttcca gcctcaccgc ttcaagcatg actttgggag atttgagcga
    41401 gcaccgggaa atacttgcca tgcgctgcaa cggaatatcc ccgattcgcc tctacctgcc
    41461 catcctcctg ctttcattga tcatcaccgc cgcaacattt gtcaccgccg attatgtcct
    41521 tcccgtttct tcggcgcgct atcgagagct ctatatctcg ctgatgcagg acctgccgac
    41581 gcttgaattg gtggatggag gaatcaacac gatcggaggc aagaccctta tcaataaaca
    41641 tgtcaatgaa aacaggattg atgaattgat tctcttcgac tcttcatccc gagaggatgg
    41701 aaaaatcctt acagcaccct cggcgaccgt gacgttggac gatccgctga atttcgtcta
    41761 tcttcttgaa ctggaccggc cccggcttct ctacacccga agcgggggcg agtgggcgct
    41821 tgcccaagcc gagcgagccc ggcttttttt ggatttctcc aaacaggtcg gttcattggg
    41881 caccgttcta ccgagtcaac taagcagtaa acagcttcaa gccgagattg ctcaataccg
    41941 aataagcttc gatgaagaga tgaagcacta taatgaacga ttgagcgctc tgcgggagga
    42001 ggtcaaggcg ggaaccaaaa gcaaagcgga actcaaggag ttcaaaaaac aaaaaccgat
    42061 caatttttac cttcaatact acacggcgga attgcacaaa aaatatgcgt tgagcacggc
    42121 gtgcacgatc ctgctctttc tcactttcgt cctggcccat cttcgtctcc gccacggcaa
    42181 actgatcggc ttcggacttt ccatgattac cgcggtcatc tactggtatc tgctcttttt
    42241 cgcccagctg cagattttca cctatccgat ctccgccgcg ctgttcatct gggctccgaa
    42301 catcatcctc ggcacgctgg ggattcttct gcttctcatc atgaggcgct catgaaactc
    42361 taccgccgct atatcttcaa aagcaccatc aacgttggtt tgggcgcact cttcctgacg
    42421 accctgatgc tcatcagcgt cgatctgttc gccaacctcg acacctatct ctcccatgag
    42481 cttccgctcg ccacgatcgc atatcttacg cttctcggcg cccctcaggc ggtgctcttc
    42541 gccctcggtc cctccctgct gttcagtgcg accttctttc tctctcagct tcatgccaac
    42601 aatgaatata tctgcctgat cggcagcgga atccggcctt tgaagatcat ccttccgatc
    42661 atagcggtcg gattcatcgc ttcgatcctc cagtttgctt ttgccgaata tgtgaagctt
    42721 ccgctttcca aagagcggct tattctcgaa gaggagctgt ttggactgcg atcgacgcag
    42781 gacaacagga acatcaccct ccacgatccc gagggacggt atgtcctcta cgcccggcac
    42841 tatcgagctc aggacaacct attgcgggag gtgctgctcc ttctcttcga tgagcagggg
    42901 aacctcaccc accgcctcga tgcccaacaa ggccggtatg atgaagacag cgggcaatgg
    42961 aggtttcacg gcgtgcaaag tcaggaggtg ggggaggtgg tttcgacgag aactcttgaa
    43021 gcctatgaca accctcggat caatctcgat ccttcctatt tcaggggagc gggcggtgac
    43081 atcgatcaaa tggatatcca aacggctctt cagtatctgc attggatgaa gcgccttgat
    43141 ccgaatcgcc atacaccgct ggctgcggat ttcgttaaac ggatcctgga tgcgttgaat
    43201 ccgctggttc tcattgtcat agccgccacg atccgatact cgctgaaaaa gaacatcctt
    43261 ctctttgcga tcatcaccag cctgatcgtg gcggtggtct actatgtcgt gcagatggtg
    43321 acgatgatcg ccgcccgcca aggggttctc tctcccgtct gggctatggt aattccctcg
    43381 gttgtggtac tattgctcgc agcgcttcag cgcacgcttt caagacaggg atgaccatgg
    43441 cgatattcga aacgacgcac caggatgcca agaccgccgc ccgcctcggg ttgctgcacc
    43501 tcggtcacgg gacggtggaa actcccgttt tcatgccggt gggaaccgcc gggacggtca
    43561 aaggaattca tcacgaagag gtggagcgga tcgggtataa gcttattttg ggaaacacct
    43621 accacctcta tctccgaccg ggtctggaag tcctcaagca gtatggagga ttgcacaggt
    43681 tctcggcatg gaatcataac ctgctgaccg acagcggggg ttttcaggtg ttcagtctct
    43741 ccggattgcg taaaatcgag gaaaagggtg tgagctttca aagccatatc gacggctccc
    43801 gtcacctttt cacccccgaa agcgtcatcg atacccaggc ggtcatcggc agcgacatcg
    43861 cgatgtgcct ggatgtgtgc accccgccgg agatctccta ccgtgcggcc aaagaggcga
    43921 tggagcttac ccaccgctgg gcacaaagag cgattgttca tcgaaatcag catgagacga
    43981 tgaaggggaa tctctttgga atcgtgcagg ggaacttcta tgaggactta cgaaaagcat
    44041 ccgccgagtt cttcaacgag caggactttc ccggccttgc gatcgggggg ttgagcgtcg
    44101 gagagcccaa ggaggaattc atccgctttc tcgcccacac cgcttcatac atgacgaagg
    44161 aaaaaccccg ctatgtgatg gggatagggt cccccgacta catcctcgaa gcggttgagc
    44221 agggaatcga catgttcgac tgcgtccttg ccacccggat ggcccgaaac ggcgcattgt
    44281 tcaccgatga cgggatcctg gtgatgaaga aagccatctt caagttcgac caaggtccga
    44341 tcgaagaggg gtgcacctgc cgggcatgca cccactacag ccggggatac ctccatcacc
    44401 tgatcaaagc gaaggagatg ctcgggggga tgctggccac cgagcacaat cttacgtact
    44461 tccatcgcct gatggagaag atccgaaatg cgatcgcaaa cgatgagttt gcctctttta
    44521 agcgagccta ccttgagcga ttctacggaa catgacaagc aaagcctcac ggtgatgatc
    44581 tgcacgctgg caagccgcct gctgggcatc gtgcgctccc gggtcttgac gaccttgttc
    44641 ggggccaccg cagttgcgga cgtgatcaac ttcaccttca acatcccgaa caacttccgc
    44701 aagctctttg ccgaaggagc gatgaactcc gccctgatcc cctcgttttc ctcgctgctg
    44761 gctgaaaaac gaaataacga agcgcagaag ctctttgctc tgctggtgac ctatcagacc
    44821 gtgctcttca ttcccttgac ggtgttcacc tatatctatg gaaaagagat cattgccttc
    44881 ttaagcgatt tcgaggccga gcaggttctc ctcggttcca agctcctgcc gtacttcatc
    44941 gcgtacctgg ggacgatcag cctgagcgcc accttcaccg gattgctcca gctgcacaag
    45001 aggtttttcc atgcgttcgc aagcccgctc tgttattcga tcgtggtcat ttcaggcgtg
    45061 ctgttgacct cttcttcctt cggagcgatg agcgtggcat acgctacgat cgcaggagga
    45121 gctcttcaag gattctatgc gtacataacg cttcgaaggg aagggtatcg attcagactc
    45181 atgttcaacc cgaaaggcac gaccttcgca cccaccctca aggcatgggg gttcgtctcg
    45241 atcgggatgg ggatgcagat cgtcacccag atggtcagct actatctggc ttccaccttg
    45301 ggcgagggga gcgtaaccgc attcgccaac tccctgatct tctaccagac cccctatgga
    45361 atcttcttca acgcgatcgc tgctgtgagt cttcccgccc tgtcgatggc gttcgcccag
    45421 aacgatcatg ccgccctgaa acggcagagc agggaagccc tctccaaact gacttctctg
    45481 cttcttccaa gcacgatcat catctatgcg ttccggctcg agatcatctc ggtgatcctc
    45541 cagggaggaa agttcaccca cgccgactcg atccgaacag cgacggtgct ggggccctac
    45601 ctgctcttca tgacgagcac cgccttccat tccctgcttg tgagactcgg tttcgcaacc
    45661 aagcgcttca aggcaatgac aatggtgcag atcgttcaga acgttcttga tatgctcttg
    45721 atggttttac tcatcaaagg aaatgcgggc cttcaagccc tctcgctggc caacggaatc
    45781 tcctaccttc tttccctgtt catcatcctc ttcatcgtga aagacctcta tacaccgttc
    45841 accgacaggg ttctcttcaa ggaggtcggg aagatcatcc tggcaaacat cccgatcctc
    45901 gtcggagcga ttctctacaa gctccttgca ccgccctggt acgcagacgg ctccacctgg
    45961 aagaacctcg gcatcctcat cgtcatcggg ctctcatcca tcttcgtatg gctgac
//
LOCUS       DAOSLI010000009        44834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_975_length_44834_cov_25.812546, whole genome shotgun sequence.
ACCESSION   DAOSLI010000009 DAOSLI010000000
VERSION     DAOSLI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 44834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44834
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_44834_cov_25.812546"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(773..1234)
                     /locus_tag="PLF67_02545"
     CDS             complement(773..1234)
                     /locus_tag="PLF67_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88927.1"
                     /translation="MNNPTTPQLRCFTSVQEYKDYFVRTICRLDIRTHDGYRVHIRPS
                     HFKHAFYEDDGKDKKGRFSFYRAKYMDWIQPTLEHPAKCHQGWDYKKATRAPYKRVAY
                     RYQTFIVVIEFRRNRRTQAIEAEFITCFDANHSFDKIARQPEWDPKVLNKL"
     gene            complement(1257..1667)
                     /locus_tag="PLF67_02550"
     CDS             complement(1257..1667)
                     /locus_tag="PLF67_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOE88928.1"
                     /translation="MDIKTFRLGELFCGPGGLACVVLKASVEGFKTEHASASDYDPDT
                     CSTYRRNITPDNPESVICENVRNLDIPSLDSIDALTFGFSCNAYSVVGEQKGLDGTFG
                     PLYTYGVKALRVSSWLSNRAYRKDGANAQYFSLI"
     gene            complement(1932..2090)
                     /locus_tag="PLF67_02555"
     CDS             complement(1932..2090)
                     /locus_tag="PLF67_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88929.1"
                     /translation="MSGVIRFGEHVKNVVAIVESARERAYHKVNEELILMYLDIGEYI
                     NVHSENAE"
     gene            2307..3398
                     /locus_tag="PLF67_02560"
     CDS             2307..3398
                     /locus_tag="PLF67_02560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE88930.1"
                     /translation="MKLSYENKEREASILANTEAAVFDGEIGGGNLLIHGDNLNVLQA
                     LRASFSEKVDLIYIDPPYSTNTVYRIGTERTGTVSFSPEDDIAYRDDLGGSEYLESLR
                     KRLIIAKKLLSPWGSIYLHIDDKIGHYVNVLMDEIFGQENFRNDITRIKCNPKNFSRQ
                     GYGNIKDLILFYSNGPDPIWNEVRVPLDEADLNRLFRKVDKDGRRYTTIPLHAPGETR
                     DGATGKKFKGLLPPKGRHWRSSPEVLEKLDAQGLIEWSKNGVPRKIIYADEHIGKKLQ
                     DIWEFKDPQYPSYPTEKNLELLKTIVAASSNPESIVLDFYCGSGTALLAARQLGRRWI
                     GIDESLPAIETVGQRLGPTYQLLKQITIG"
     gene            3474..3926
                     /locus_tag="PLF67_02565"
     CDS             3474..3926
                     /locus_tag="PLF67_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88931.1"
                     /translation="MKRILIIAVLALIVVFPVAAASTSWAAGLNIGTGASLAGQFQMD
                     KFKLNVNLGYGFVNKYVAADVFADYQVYEFNINKARFGVTAGAGANLAMVNKAFGLAA
                     IVPVGLVYRLENRDFPLDFYLRVQPGVKILPDLGFHIDAYIGAVWRFQ"
     gene            4073..5251
                     /locus_tag="PLF67_02570"
     CDS             4073..5251
                     /locus_tag="PLF67_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HOE88932.1"
                     /translation="MCDTIGRIMDEQSVLFGKNSDRHPKEVQVLEFVTGTLESDRYPA
                     LEKYTPQFETLEKAHRHFEHPYPALISRPSWIWGAEMGVNDRGVVIGNEAVFTKQRSL
                     KEGLLGMDMLRLALHNASTAEAAVEFILNLLDCYGQGGDGAFIGKLSYSNSFIIGDKN
                     RLFILETAGSRWAVKQIKTHDAISNAYRIGTSYERSDAVSSGKHFAETWRDGFMEFFS
                     KGRVRQKTTSNLLADAEPTWMGMRDVLLYNRGTLGKMDRSMKSIAINASFPKPTRTTA
                     SMVVEYPEKNILIWSCPAPLPQYHPFVPIIFGIDGIWEEKNSYEDAKKRQDLTNALLR
                     SSAQERIKAADAARTLDKRFEKRIRTALGDKAKLKKAVEQCTADFHKHEEDLAKRLGK
                     "
     gene            5477..7306
                     /locus_tag="PLF67_02575"
     CDS             5477..7306
                     /locus_tag="PLF67_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88933.1"
                     /translation="MNKKLFILVLVAIFSASLFAMAAGETVAPKVETQVILGSSTQVN
                     ADFFSGWTNSATNSYLKTLMGGYETVSWTKEGRYAINPTAVKSWKATDNADGSKTYVF
                     NIANNLVYNDGTKITAKDYVFNILYAASPQVVEIGARYGVGPEYVGYAAYHDGSAKEF
                     KGIRLLGDYSFSIQIAAEELPYYYDLSLVSVGPLPLHVIAPGVTVTDTPNGAKLSDNF
                     TTDLLRETVLTPNTGYRYRPLVTSGPYQFESYDPADSTASVILNPKFLGAYDGAKGNI
                     YRIIIKLTNTATQMDELKTGAIDMLQGISGGAQITRGLDLHDQGIVNFASYPRYGYGK
                     IAFACDFGPTQFPAVRRAIAYSLDRNEFARQYTGGYGIVVNGYYGASMWEYRENKDRL
                     DKELNSYAYNLQKAEDELVKDGWTLNKDGKAFVKGVDQIRYKKVDGELMPLSIEWANT
                     ANNPVSDLIAAMLVPEVKKIGMQINGTTVDFAVLLDHLYKDGIEKPVYGMFNLGTGFA
                     TTQAIWYYYSTDPEYFGSYNTNFIADKELETIALDMKRTDPQDQKGWAEKWILFQKRW
                     NTLMPDIPLYSDIYHDFFNPKIVGYDSSPTWQFQYAILRADVK"
     gene            7379..8362
                     /locus_tag="PLF67_02580"
     CDS             7379..8362
                     /locus_tag="PLF67_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88934.1"
                     /translation="MFKYVLRRLMYIVVVFLIGSVLIFSLYKSVPGDPARLMIEGNKA
                     NMKPAEYEALYRDAVKKLGLDKPLVVQYFTWLFNTLRGDFGYSLTYKMPVRQMIGVPM
                     KNTIMLNVWSLLPVFAITIPLGIATAVRRNTRFDSTVQVITIIGYSLPFFVIALLFIF
                     LFAVKLPIFPISGVQTPGLRGTPGRMFLDKLYHMALPLLVMVFTSLGGLTRYVRATMI
                     EALSMDYIRTARAKGLTEKVVIYSHAFRNALIPFITIMTGWFISIFSGSVVIERTFLW
                     NGMGKVIIDALNQQDFAVSLAMVMFYLVLSLVGNLLMDLSYGLADPRVKLN"
     gene            8375..10171
                     /locus_tag="PLF67_02585"
     CDS             8375..10171
                     /locus_tag="PLF67_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE88935.1"
                     /translation="MAKKPLGSTFTDVLKTFFLGRQRATLSILEEEQVQSPYRTMFRN
                     FREKWSAMLGVYMFLAIFLAVFILPIFFPLDTQFQDPTQQNTRPTFSYMKYDKALQGN
                     VASIDGGSVFGVGVDRDGKLYQWGTLSAKLQKMPPTDKRYVQASAGLDHAVALDADGK
                     VHTWGNDRFTLNMIPPELSSRKVKQVIAGHQISIALTEGGEIYVWGNSNLISIPVPKD
                     KQGTFTKVVANTTLAAAITGNNELVILSSRETPFSYIPSEIQGRVRDVDLTERSAAAV
                     TTDGRIYVWGGNDYGVMDIPAHAQGRAIAVSAGRGHFVALLDDGSVVAWGRNNYKQTN
                     VPKLKNIVSIQSSYYQNYAVDKNGRIHTWGLKGYLMGTDQYGRDLFVRLLTGGRVTLT
                     IGALAVIISAIIGIIIGSFSGYYGGKTDMFLMRLAEVVNAIPFLPLAMILSAIIGSRI
                     SETGRISMIMVILGLLNWPSLARLVRGQILAAREQEFVTAAKAMGITEMKIIFKHILP
                     NVITVVLVNLTLSFALSMLYESSLSYLGFGVNEPNATWGNMLYGCNDSTVMSLYWWRW
                     VFPAIALSLCTVSINMAGDGMRDAIDPKSIGR"
     gene            10209..11174
                     /locus_tag="PLF67_02590"
     CDS             10209..11174
                     /locus_tag="PLF67_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE88936.1"
                     /translation="MHTYFTTKRGIVKAVNGVSYKVEAGKTLGVVGESGCGKSVSAMS
                     ILKLLDGNGYIAGGSIIFKGQELTTLSEKEMYEVRGNAISVIFQEPMTSLNPVFTVER
                     QISEAFMVHQNMDKKTAARESVRMLTDVQIPNPEEVVKQYPHQLSGGMRQRVMIAMAL
                     ACKPDLLIADEPTTALDVTIQAQILRLMNNLQKEKGTAILFITHDLGVINQMADDVAV
                     MYSGEVVEMAPVDVIFGESKHSHPYTEGLMLSVPRLDTASQSKLETITGSVPHPLDLP
                     KGCFFAPRCKYATKKCQTEHPELVSVGKQHTVRCFYPEKGARSGK"
     gene            11164..12702
                     /locus_tag="PLF67_02595"
     CDS             11164..12702
                     /locus_tag="PLF67_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE88937.1"
                     /translation="MENKNVLFKIRGLKQHFPLKKRGLVVRAVDGVDVDILRGETLGL
                     VGESGCGKSTFGRVLLQLYRQTDGKTLYYGRSLEDLAPAYVKNTYTHLAKHKNQLQIL
                     QKAFEDAAAEYERLKDDESQKAEVVYTALNKSNEAEKAYRNAYLDIVQLIGGLFVVED
                     VEEIKALLLDTYEASFERHRVDEQLKDIALKIAETQRSHNRKPASSKEKRIASLKEIE
                     ATLKNALETAASNIVEANKKLEQKRKEYRHVPGFDEHESYRDEGIDLSRLTFNEMRLL
                     RNDLQLIFQDPYSSLNPRMTVGQIIGEGLLAHRFFKKDGPEMQEYVMKVMDECGLAPY
                     MIHRYPHQFSGGQRQRIGIARSLAMNPQFVVCDEAVSALDVSIQAQIINLLRDLKEKS
                     NLTYLFISHDLAVVKYISDRIGVMYLGNIVELAPAESIFTNMMHPYTEALLAAIPTTD
                     RGAKKEPIILEGDIPSPINPPSGCKFHTRCRYKTEICTMVEPEWRELGKGHFVACHHP
                     LTEK"
     gene            12704..12889
                     /locus_tag="PLF67_02600"
     CDS             12704..12889
                     /locus_tag="PLF67_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88938.1"
                     /translation="MLFPKKIRRVLDVRKAEEKELTEELEKGDIPAMIIAAFLVFTPV
                     ILLIIGIVWGLYWLFTH"
     gene            13218..14543
                     /locus_tag="PLF67_02605"
     CDS             13218..14543
                     /locus_tag="PLF67_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE88939.1"
                     /translation="MEWKESWHDEYLKWICGFANAQGGKIYIGKRDDGTVVGIQNAKR
                     LLDDLPNKIQNKLGIVADVNLLTEGEHEYLEIVVNPWSFPVNYDGAYYYRSGSTKQLL
                     QGNALTGFLMEKTGRKWDAVTITNIGVEDLDRESFDIFRREALRSGRMTKEDVDISNA
                     ELLEKLDLMADGKLKRAAALCFYRQPEKVSSGCYVKIGKFEGSEIVYQDEVHGSLLIM
                     ADRIIDLMYLKYLKAAISYHKETRVETYPFAREAVREAVFNALIHCNWADNVPVQIRI
                     EEDVMYVSNCSMLPFGWTVETLLDSHTSKPYNPDIARVFYRAGYIENWGRGIQKIRDA
                     CKAHGAEEPEFIVHGGDIMVKFKALQSAIVTDSKVSNVTKDVTKEVPMEEAILCLIRD
                     NKDITTTEMAEYLAVNRRTIQRVLDDLKNKDIVKRKGGKRYGHWEILDQ"
     gene            14824..16356
                     /locus_tag="PLF67_02610"
     CDS             14824..16356
                     /locus_tag="PLF67_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE88940.1"
                     /translation="MNNDSIYSGRYVLPAPGTPAGYTALINHYHLKTPYPSLKMAIGE
                     GYAQQDHDGWRFLSKRVRIEPTLGSQLTFALRYEGINLLVLKKLFEAVPVQEIEDVVK
                     ESITGQYARRIWFLYEWLMDRRLSLDDVTQGNYIPVLDPKQQYAVAGIRSPRHRVIDN
                     LPGTPAFCPLVTKTPELLEFESLALQDRASTLVSTIPKEILSRAAAFLLLKDSRASFT
                     IEHESPTPDRLARWATAIGQAGRVSLSKEELLRLQELVIGESVFIKPGFRTEGGFVGE
                     HEPMTGFPIVEHASSRSEDVIPLVEGILAYEQRTRDSFDPVVSAASVAFGFVQVHPFS
                     DGNGRIHRYLIHHILAQRGFNPPGIIFPVSHAMLKRAQEYQRVLRAYSSSILPFIEWT
                     VTPDYNIHVLTETADYYRYFDATEYTLFLYRCVQDTIEDGFEQEVSHIIAYDRFQAGL
                     QRLGEMPDRSVQLLYQFLRQHNGTLSKRAEGKEFKELSVQMIKEIEAIYAEAFGTGSS
                     LE"
     gene            16408..17214
                     /locus_tag="PLF67_02615"
     CDS             16408..17214
                     /locus_tag="PLF67_02615"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE88941.1"
                     /translation="MNVLITGATSGIGFETALLFATNGAHVWGASRNPIASPSNRIVF
                     RRMDVTNEESVKKSVEEIWNEAVKTTGKGIDVVVHSAGYGIGGSAEDTPIDDAIAQFD
                     TNYFGVLRVNRVLLPRMRAQGSGRVVILGSIAGRISIPFQSHYSATKFALEAYTEALR
                     LEGAPFGLTATIIEAGDTNTAFTANRRLTIDPSSPYHDQALRSIARMARDEELGYPPT
                     KVAKMIYKVSQMKNPPIRKPVGLSYSLLMLLKRLLPDSLAERIIKAMYMR"
     gene            complement(17211..18185)
                     /locus_tag="PLF67_02620"
     CDS             complement(17211..18185)
                     /locus_tag="PLF67_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88942.1"
                     /translation="MSTKVLGGDERLFKKDYKAFFKEWMILFLFIALFIICSIALPKF
                     RTTNNIMNIFRQASFISIIALGEFFVILIGHMDMSITSIIGMTSIFFAGMVVNAGIPM
                     FWAIVIVFALSIFVGIVNGVLTIYGNMPSFIGTLTVMNIIKGIYFIYSRGLPISGLPD
                     AFNFFGAGYVGFVPFPVILLLIVAIILYVFTQHTELGRSFYAVGGNRVASKLSGINTN
                     FIGILAFVICAVLSCIGALGLTSKTLSGNVSIGENLLFDVMTIVVLGGTSLTGGRGKV
                     FGVIIGALFLQVISNVMVLLSINSYWQWVVKGLILIVVVLIDANSKHD"
     gene            complement(18182..19711)
                     /locus_tag="PLF67_02625"
     CDS             complement(18182..19711)
                     /locus_tag="PLF67_02625"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE88943.1"
                     /translation="MVVEAKAVNVETLVEVKNCTMVFPGVKALDNVDFTLLAGECHGL
                     VGENGAGKSTLSKCIIGDNRMTEGELYIQGNKIHIPSFNVRKSQEMGIAIVHQEFQLM
                     GDLNGLENIFIGRYTKRGPLIDWKELQKEGEALLEYLQCDVDLKVKVKHLRTAEKQII
                     QLAKAMLDNPKVIIFDELTSVLQEDGIENIFRIIRLLKEKGIGIIYISHRLDEIFECC
                     DRYTVLCDGKWINSGYVKDIDKPELIKMIIGRELKNVYPPKNEEFGETLLEVKNLTAH
                     DGSFRDVSLSVRAGEVVGISGLLGAGKTELIHAIYGNYKTTSGDVFVRGKQVEIKSPQ
                     HAIKLGLGVIPDERRLLGLNMLFNIQDNTTLPSMFMFKKWKIFQNHRAEEKAAFDIND
                     KLKLRYVSLSQLISKLSGGNQQKVVIAKWMLRNCDLFLLDEPTRGIDIGAKFEIYSLI
                     NELAKAGKGIVLVSPELEEIIGLSNKVYVMYEGKVMDTVEGDDINQEVIIQDLLGVKE
                     A"
     gene            complement(19702..21744)
                     /locus_tag="PLF67_02630"
     CDS             complement(19702..21744)
                     /locus_tag="PLF67_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOE88944.1"
                     /translation="MDNKKETKSFRKIRIDTTDSDLLTLAVTRFWNYPELFDDPGNDE
                     IVVTLIPSLENKKHSEKPELRSQGYALEINKEGITLSYEGRAGYINALTSIKMLLRQG
                     KNGYTLPCCEIEDWPSVAVRAVAPTFSWYAGYGRIGFDSQLWGYDKWVEFLNVCMDNK
                     INQFNMVMYGYWPFEFEKYPETVYRNVPVDIWNAENDDWLTIRYTHPNIEHPYLTKLF
                     DLGHMMEFSFFAYVGLNSYNGAYSIKHPEARMIPPKGGQFLNDFDSLCLSNEDNVNYI
                     LDSMQRIAEIGFDGFTLEESEEGFWFCECDGCRTRWGETSEAPVEAKHKANIWLLNQI
                     WDRVKSVNKDAVLGIRAFRQPPLEKDPAFLASCVDEMPKGINLFWAPGLYVPESEFEK
                     WVNAFGKEHIWARDTEANSITSTMGRLYRTFASNLIRYEDETNEQVIETDIRQHLGST
                     AMGVHGINGYMFEWYGLFLHLFAHGNYGWGSRMEMDEFFDKACELNFGDLGKDVLYVM
                     RTMLTIHQSQMPLYTTPFPFQKNKIRNSDIPEIEAAKARHPKIFEKLTNLHKACLADP
                     KLRHWAPHFAKLKNAERRNMVIYEMIKASLRYEDEKDPKKKDRILDEILAYNEEDFAI
                     AKEMFFDINPVGETGVKSSMFPYHEIKRLIHNIRNPENPDNDIICSGIEALGWLWL"
     gene            complement(21849..22850)
                     /locus_tag="PLF67_02635"
     CDS             complement(21849..22850)
                     /locus_tag="PLF67_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOE88945.1"
                     /translation="MKKGMVFVMIFLLVGSMLFGAGRAEEAAKETKIGVSIMELSAYT
                     WYLGVIDGCKQWAADNPDANFTFQFEDSRSDVSTMLNNIDALITGGAKGIILFPADAS
                     SAIPTMKQYVSQGIPFVIGDYAQDPKSPNDIVWETFVGHDMRMLGETAGEVAVEYLKK
                     LGKPDPVCLFVTRPTSGQVSVDRFEGFRDVVLASFPKARIIEEGDVGAGSRDSSQSLM
                     ENVLQREPVIDVVSGHNDAEVVGPYNAARAANRKEIKFIGIAGDKDVLGYIDSGNEAW
                     LGEVLQNPVDLGYQATKAMYQALIEKKTLDKVWDLPKPEAITPANIAQYDWKNWSWL"
     gene            complement(22911..23648)
                     /locus_tag="PLF67_02640"
     CDS             complement(22911..23648)
                     /locus_tag="PLF67_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOE88946.1"
                     /translation="MDLIDMYFDQIEDAKQRRVPFIIPVGTIEYHAHHVSSGTDTMVV
                     TGCLRELGKEKEIVVLPPIWYGVASYAVGGPETGTIHVDEDTHTQYVYCILKSLIWGG
                     VKNIYLIPHHQTEAGALMPMTISCHKAAKKVTMEYMEATKGVGWWGSNDYQDYYENLG
                     SGDDPFSYIKVIPLISKEAQIACGGFDHAGKWETSLMMGTYPDHVDLTRCKLNTEWFA
                     QSAVEATAEIGKHMVNSVLDWLREAIV"
     gene            23869..25050
                     /locus_tag="PLF67_02645"
     CDS             23869..25050
                     /locus_tag="PLF67_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE88947.1"
                     /translation="MLQKRINVGLVISLAGFAISVNTLPPLIEWFSGHLCMPISSFGL
                     VYFFQYGAFTLCSIGIGKLHAKRGLPLLSIAIGALFFSALCVFLIGRATSFVLLITLM
                     MLIGGAGGLVESIGTTLLTVEDGSNRMLHASQLFYAIGAVVAPAMVGFLLASGVHVPK
                     ISQYIGIFSLLIAIVVFGLTRSSNSKTSKETPTPSHRPATAPLFALYVLFLTMGAYVI
                     VEMSLGNWLAVFAIQTFSVTEAQGSLALSLFWTGLCLSRLFYVLKSVKSQQKTLSIHM
                     ALMLLSCLILIASTKIGTLTLLYPTVILFGIGCGPIWPLLIEFCSAAFREEHLVMYLV
                     ASGSIGALLGPILTSTLFGFFGIRNIPMILSAYTVLMMGSGVLTFRILRNARRTRRSA
                     A"
     gene            complement(25022..26050)
                     /locus_tag="PLF67_02650"
     CDS             complement(25022..26050)
                     /locus_tag="PLF67_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE88948.1"
                     /translation="MSTKKNNKVRLVDIARETGFSIKTVSRVVNNQGDVSPKTRQAIM
                     KVVNKYNYSPNPMAATLRTRRTRTIGLVVPDFTNEFFGEVGLAIEAALRAQSYSLIVS
                     FSGNSHEGEVRALELLSSKYVDGILLASVGTTTDAINELLKEEMPLVVFDNEPPQVAT
                     NVVLHDNINGAYVLTRHLIDHNYRSIGCVAGLQHQSSGSERLEGFLKAMEDNRLEAPA
                     SHIIVEDWTSEGGYRAMNQLLGLPRSERPRAVFISNSIMALGCYKALIQAKVRIPEEM
                     ALVSFDNLSFMESLEPPITTLSSTGHSVGEQAAKLLLATLSNEDSPPVERITIQGTII
                     KRRSCGCA"
     gene            complement(26047..27129)
                     /locus_tag="PLF67_02655"
     CDS             complement(26047..27129)
                     /locus_tag="PLF67_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88949.1"
                     /translation="MKKNNPTMLNLNKRFFGPVKQTILQSRAWSIEGFAYQSGVEAVT
                     IEVGRGVFTWTPFMGSELWDWSVDGVSRKFEGFRKEPVYQAPFLANYGAFLIHCGVRG
                     MGHPSMEDTHPHHGELPVSQPVRAWIEIDEEDKEFPLALCAEFHSHIPFVGEYSFVPA
                     LRIHKSGTFVVVESGLTNLAHAPFEYQYLGHVNFNYPKSGRLEYSIANFTDREVVVLD
                     ETFEGVTKKPELFLTLDKGCTLDPELVAVIDYGKKSPSTFRNGKFALSRMSDEKGGST
                     WVVARTDILDHTVIWLTQTPDRKAAGFALPATSGPTGFTNEKKMGNGKVLPGKQSITL
                     WYAFGVGDGDIEKDIATALSRSGMTE"
     gene            27539..29035
                     /locus_tag="PLF67_02660"
     CDS             27539..29035
                     /locus_tag="PLF67_02660"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE88950.1"
                     /translation="MKRELLVMKNIDKSFPGVHALDHVNFDLRSGEVHALLGENGAGK
                     STLMKILTGVYQMDGGEIEVAGEPVTITNAGDAQKLGISIIYQEFNLFPHLSVAENLF
                     IKREPRKYGKWILDGKKLHEQSEELLQRIQLNLDPARPVSSLSVAQQQMVEIAKALSL
                     NAKIMIMDEPTSALTDHEIENLFSLILQLKKQNVGIVYISHRLEELSHIADRVTVLRD
                     GKYVGTVNYSDTTTRELVSMMVGRELSEYFPQREHAYGAPILEVEGLSRKGAVDDVSF
                     TLHEGEILGVSGLMGAGRTEMARLLFGADRKDGGTVTFKGEQIEISSPGDAIKRGIGY
                     LTEDRKRDGLTLGLNVKDNITMASIKEYASMLGVMNEGKIEKATSEYVDQLSIKTPSL
                     EQLLRNLSGGNQQKVILARWLCKGSKILIFDEPTRGIDVGAKQEIYELMNSLVKEGHS
                     IIMISSELPEVLGMSDRILVMHEGRVAGILDGPSATEESIMYLATGGK"
     gene            29057..30013
                     /locus_tag="PLF67_02665"
     CDS             29057..30013
                     /locus_tag="PLF67_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE88951.1"
                     /translation="MKKLELRASTLQQFAALGSLILMMILFSILSPYFLNFDNLITVI
                     KQTSVVGIIAIGVTLVIVTGGIDLSLGSVVALSGVVAGFALAKGASNFVGILIGALIG
                     LLCGLISGVIVAYLELPAFIATLGMMMSARGMSLVLTQGRPVYIPNNKTFNLIHNYNL
                     FKYIPLPVIYFAVIALLTHFLLKRTSTGRYLFATGSNEEAARLSGINVRLVKLFSYGF
                     CGFMCGLSGVILLARLNSAQPAIGSGYELDAIAAVVIGGTSLSGGVGQISGTIIGVLI
                     MSTMKNGLNLMHVSQFWQQVIIGGVVILAVYIDIKRREAQRL"
     gene            30060..31016
                     /locus_tag="PLF67_02670"
     CDS             30060..31016
                     /locus_tag="PLF67_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE88952.1"
                     /translation="MKRIVTLVLIVALLVPTVLFAAGAKEAAAKETYIAVISMGYMHE
                     YWQAVRLGTENAAKELGIRTTYEAPPSEDMVDLQINMVENAIQTKPDGLLLAPLDAEA
                     LVPYIDKAVKAGIPVVLFDAGANTDTFASLVATNNENAGAIAAQNMIRLLGDKGTIGL
                     IVHSATDQSAIKRRDGFLNYMKANSKINVLPPVYSGGEHEESANLMVDLLRANPDIAG
                     VYATNEGAAVGAGIGAKEAGKAGKVTIVGFDSGAQQISFLEDGTIAGFVSQDPVTIGY
                     LGVKTLYDVLQGKPVEVEIDTGAVFVDLSNLHDDHVQQIIYQ"
     gene            31090..32262
                     /locus_tag="PLF67_02675"
     CDS             31090..32262
                     /locus_tag="PLF67_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOE88953.1"
                     /translation="MKKRVTIPEHEYFERIAKAAKLTGEAGLDILISNCTEADYSFVR
                     YFTNFWPLFETAGVAIAPNGKAALMVGPESTKFAGDRSVLKTIFTLLEYRESADPAYP
                     EFKPQTFKDVFKHLGVTGKKLRIGIAGYLLTNPIQLEGLRSNFPDAEIVRADDIVRQL
                     RVIKSENELACMREGFRIVELATKEVLKAIRPGVTELQMVGVAQKTIYEHGAEYEGLP
                     MYVFSEKSTSHAISRSTYREIGKGDLVQLNLSAKIEGYSPSVGMPISMGPLVGEKREL
                     VEFGLDMHRWTYKQLKAGVEASSIAQGYYQRFIDLGRKDNFVYGPCHGTGMIEVEAPW
                     MESSSTYKLQPNMTFQVDTFASGPSYGLRWETGVAITEDGYDLLSNPIGEIYEIDC"
     gene            complement(32379..32750)
                     /locus_tag="PLF67_02680"
     CDS             complement(32379..32750)
                     /locus_tag="PLF67_02680"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOE88954.1"
                     /translation="MKNTIGKTIWVFPDGDLPPAGPFPLKGHESVIILNMNEQAVDVK
                     MTLYFTDKDPVVDIPVVVEANRVRCLRMDDPSDLAGFVLERDTQYAIKLECSLPVVAQ
                     YGRLDTREQPLSFYTTPGYSQ"
     gene            32930..35209
                     /locus_tag="PLF67_02685"
     CDS             32930..35209
                     /locus_tag="PLF67_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE88955.1"
                     /translation="MATIKDVAKKAGVSIATVSNYLNNTNPVRKETATRIQAAIDSLG
                     YKQNYMARNLKAQRYTDVGVILPDLDNPYYVQIFQGIEKTFHQSKYYLTLAFSYDDPE
                     REAEIIDNLLKKQVAGLVLVPSMVDNWKFYYHHLVSRDKPLVILDRQIDNLETNFIAF
                     ENEKAIADLAKSLIDQGKRNLVLITGPRKYREEQLCIDAFLEVCSQNDIDINQALQVY
                     SVGTSKEETFRITTNLLRRFLPDAILTTNGAGAVGIIESLSFLGYSTNDIPVATLGEE
                     RWNKYTHSISSLSSQRPAIQMGEQAAQLLLEQLKSPFVSENRRILMTDKILTKEPHPV
                     RYAQYQPIQIDRNGQTLKVLLLDTYQTHALSGLFSNFTSATGIAVDATSIPHPELFER
                     IIQESRKAPGDSPFDVVMFDIPWLYTLASSKVLADISPHIERKEINPGIFLPRSFEYF
                     SRFEKGHYGLPFMYAPQILFYRKDLFEDPFNKTEFDRLFKSKLRPPQTWKEFNAICEF
                     FTSHTDHISYGTSIPASHPEYLAPEIYMRLFSSNSQVFDKNNQVTFSNPRTLKAYIDL
                     IKVMKHVKPNYLQTSDMDAVKDFLLGETAMLITYPSFLYDEELISANPGILNNIAHSN
                     IPGNAPILGGWSLGIFAQSEQKDQAFDFIKWACSDKISDYFALMGGYSAVESTYLNDE
                     LTKLYPWFPLYHSTYKTTRPILPPRTASGKIVSQNKIDSIICHGVYELLKDSSDIPSV
                     IEKTHKALEELILSHEETT"
     gene            35346..35609
                     /locus_tag="PLF67_02690"
     CDS             35346..35609
                     /locus_tag="PLF67_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOE88956.1"
                     /translation="MPAIRPSSDLRNNYNEISEFCQQYTEPLFITKNGKGDLVVMSIE
                     TYELLTGKMELYNLLDEGLKARDAGQVEPMNRVIAEIRASLKK"
     gene            35609..35932
                     /locus_tag="PLF67_02695"
     CDS             35609..35932
                     /locus_tag="PLF67_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE88957.1"
                     /translation="MYEIQWTSLAKEDLILATSYISGILKAPATAGKLLDTIEHEVEA
                     LSENPYMFSTSRDENLAHRGIRHFSVKNYMLFYTIKEETKTVTVLRFLHARRNWIQLL
                     GHPSL"
     gene            complement(36072..36740)
                     /locus_tag="PLF67_02700"
     CDS             complement(36072..36740)
                     /locus_tag="PLF67_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HOE88958.1"
                     /translation="MSTGTSRVSDLANKLGVQSGSLNPYLNNLMDLGIIERKTPVLDR
                     KTKRPVYAVSDTMFQFWHAFVQPNLSLINLGLGKKVYRQRIEPRINEYMGKVFEKICL
                     QYYEQRLKNDRVPFLPEDYGTWWGNDPLRKQESEIDILSFNAEAMLFIECTWRNEKAH
                     RKVLAELVEKSKMFKTDRKFYWIISMSGFHDVEHSLINVEMIDLKQVYSLQIERTLRE
                     STSL"
     gene            36888..38042
                     /locus_tag="PLF67_02705"
     CDS             36888..38042
                     /locus_tag="PLF67_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOE88959.1"
                     /translation="MILPEHIPTLFKEELTTSILPFWLKHGLDPVHGGMLTGLGRDGS
                     LLESDKSIWFQGRAAWTFATAYRVARKNEEYLLVAKSCLDFLKNHGQDDDGRFFFRVS
                     REGNPVIKRSRYVFSELFAALGGAAYARTTGEDSSWAETIFDQALGTVEAGILQPKFL
                     RPSEGFALPMIVIAVAQELRAANPKRTKHYNALIDQLIEKIQRLFVNEEHRCVLEQTG
                     IDGTPQFDHFEGRLLNPGHAIEGAWFIMKEARLRRDRDDYFSLGKKMLDWMWEWGWDR
                     EYGGIIYFRDVLGHPSHEYWHDMKFWWPQTEAVIATLLAYHQTGDDHYLTLFNQAYDW
                     ALRHFKDERYGEWYGYLHRDGSLSVDLKGNLYKGPFHIPRMFLEGIELFS"
     gene            complement(38039..38656)
                     /gene="hisIE"
                     /locus_tag="PLF67_02710"
     CDS             complement(38039..38656)
                     /gene="hisIE"
                     /locus_tag="PLF67_02710"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOE88960.1"
                     /translation="MQSMAVSFEKMGGLVPAVVQDAVTRRVLMLGFMNEEALKTTRDR
                     GLVTFWSRSRETLWTKGETSGNYLEVRDIIEDCDNDTLLIKAVPTGPVCHTGKDTCFG
                     EVNNPEEVSAPEFLFYLQEVIRERREFPLEGSYTNHLFSRGLNKIAQKVGEEAVELII
                     EAKDDNKQLFLGEAADLIYHFLVLLTQKEVGLEEVIEVLKLRHSR"
     gene            complement(38661..39410)
                     /gene="hisF"
                     /locus_tag="PLF67_02715"
     CDS             complement(38661..39410)
                     /gene="hisF"
                     /locus_tag="PLF67_02715"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOE88961.1"
                     /translation="MNRKRIIACLDVKDGRVVKGVNFVGLSDAGDPIGLARHYSELGI
                     DELVLLDITATVEKRKTFIDLVRGVSEGISVPLTVGGGIASVEDAKALLEAGAAKVSI
                     NSMAVRDPSLIAAIANAVGSERLVVAIDAKEGRVAVQGGRVVTDRKVVDWAKEVERLG
                     AGEILLTSMDHDGVKEGYDIALTKAVAAAVSIPVIASGGAGTMEHFARVFEETGVSGA
                     LGASVFHFGTIDIPKLKIFLAQRGITTSTGF"
     gene            complement(39407..40126)
                     /gene="hisA"
                     /locus_tag="PLF67_02720"
     CDS             complement(39407..40126)
                     /gene="hisA"
                     /locus_tag="PLF67_02720"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOE88962.1"
                     /translation="MDLIPAIDLIEGKVVRLAQGDYDQKTIYSSNPLEVAKAFEDHGI
                     RRLHVVDLEGAKGGGIVNLETLETLARNTALIIDFGGGIKKEEDLRQAFDAGARYVTV
                     GSIAVTEPALVKEWIQRFGLDRLILGADSKGGIIRTHGWLKESGLDVQSFIESYLHEG
                     LDTVICTDIAKDGMLSGPSYELYGKLLGAMPSLKLIASGGVSSIRDLERLRDLGMDGA
                     IIGRALYEGTITLDELARCNG"
     gene            complement(40126..41253)
                     /gene="hisB"
                     /locus_tag="PLF67_02725"
     CDS             complement(40126..41253)
                     /gene="hisB"
                     /locus_tag="PLF67_02725"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOE88963.1"
                     /translation="MHKKILFVDRDGVIVVEDQVDSFEKIRYIPGVFGGLKRLVDSNQ
                     FYLVLVSNQDGVGTPSFPLEDFEPVHTRIKETLRGEGILFDEEHIDYSLPADECPGRK
                     PKTGMLASFFDGSWNLEHSVVIGDRLTDVELAKNIGCKAVWLTPKSDEPKLPGDLHDT
                     CILVSDRWGEIASFLLDEPNLAPRVSHITRKTKETSITLSLSLDGGGLGEIHTHIPFF
                     DHMLDQIRRHSGCDLSLHADGDLMIDEHHTVEDVGIALGEAFRSALGEKRGINRYGFE
                     VLTMDEVKAEVALDFSNRPAFSYDVPIAREYIGTFPTEMVEHFFKSFSDSARCNLHMK
                     VGTGNAHHQVEGLFKAFARAVRQAIHRNPYSDELPSTKGVL"
     gene            complement(41246..42322)
                     /gene="hisC"
                     /locus_tag="PLF67_02730"
     CDS             complement(41246..42322)
                     /gene="hisC"
                     /locus_tag="PLF67_02730"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOE88964.1"
                     /translation="MNELLRKNIVNLKPYSSARTEFTGKADVYLDANEHYRDFVEMKD
                     ANRYPDPKATVLRKKIEEVLGLPFEHTVLGSGSDELIDNLIRAVCTPGKDSILILPPT
                     YGAYRVFADINDVEVEELPLLPSFEIDLPALERFLSKERNERSEGRLKLLFLCSPNNP
                     TGNSYSLETIEAVLKIFDGLVVLDEAYYDFSEGESGVSLLSKYDHLVVLRTLSKAWGL
                     ASARVGIAVGSRELVEVINKIKYPYNLGTPSQALALRALENAEAVKRSLDEIKRERSR
                     LSGALAAFGCVRIVYPSDANFLLLKVDDATRLYTFLTENGTIIRNRTKERGLENCVRI
                     TVGSQAENDILLEGFEAYMRKYYA"
     gene            complement(42319..43629)
                     /gene="hisD"
                     /locus_tag="PLF67_02735"
     CDS             complement(42319..43629)
                     /gene="hisD"
                     /locus_tag="PLF67_02735"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOE88965.1"
                     /translation="MPYLERFDNPKGKNLDLFCTRNLLDRSSVEEQVATILAAVNEEG
                     DAALHRYSERFDGVVLSSLAVSSEEFEEARKAVDPDLKEALALAKSNIRAFHEAQRAV
                     GEEIEVMEGVSLKRKVVPIRRVGLYIPGGTAPLFSTVLMLALPARIAGCREIVLATPP
                     DKEGKIHPAILYAAELCGVDEIYKVGGAQAIGALAFGTETIHRVDKIFGPGNRFVTEA
                     KTQVAQGACAIDMPAGPSEVMVVATTRSNPAFVASDLLSQAEHGPDSQAMVVLIGSDP
                     DRWIEELEEQLERQCSVLKRSGYVKQSLEASRIAVVQEVEEAAFIANAYAAEHLIINT
                     GDRVLDDSLVDLIESAGSIFVGPYSPESAGDYASGTNHTLPTGGWARSMSGVSTDSFV
                     KKITVQELTGDGLARLAPTVMTMAAHEELEAHRRAVEIRLEEER"
     gene            complement(43632..44495)
                     /gene="hisG"
                     /locus_tag="PLF67_02740"
     CDS             complement(43632..44495)
                     /gene="hisG"
                     /locus_tag="PLF67_02740"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOE88966.1"
                     /translation="MDEVLRIAIQKKGRLSEQSLEIIKQCGIKFGADERVLKDRATNF
                     PLEFLYVRDDDIPSYIADSIADIGIVGRNEFDEQGIDLAVLRDLGFAKCRLSIAVPHE
                     FGYKDLSSLEGKRIATSYPRILESILAEEGVKANLVSVSGAVEITPLVGVADAICDLV
                     STGATLAMNGLKEVQTIYRSSAVMIGRKEMGSKVKEEILSRLLLRIDAVMKASSYKYV
                     IFNLPEAQLEAVAAVVGGMKSPTVTKLMETGWVSVQIVVAEDTFWEVFEQLKALGAQG
                     ILVTAIEKMTE"
BASE COUNT        10585 a        12496 c        11365 g        10388 t
ORIGIN      
        1 cgaacaccga cttatcaaag ggtttcaacc ccatctcatc ctggacgata taagccagag
       61 ttcgagcgct tttacgcatc tcgcatgcgt ctggcttaat gaaaaacgaa tagttttcaa
      121 ggttgatctg cattcttggc ctccaaaaga agcctatcga gtttcaacct tgaactggag
      181 agggatttat tggtcgctta cgatgataca gtgaatgctg aagagagtaa aatagttata
      241 aaggcgtctc cacgtggaaa tgattttgag atggactgtc cagctcaatt gtgtcagcac
      301 tgctgacact ttttccccct atggaataat ctattttcga ttactctcgg aattcttata
      361 ttcgaggcac cccggcggga tggaaaggtt aagaaagagt tgaccatttt caaagaaagc
      421 tgtactattc atatgtgctc ctaattgttt gtaggtaaac aaattatagc acactgaggt
      481 tgcttttgca acctcttttt gttataaatg ctttaacaat aaacagataa aaggagctgc
      541 cgcaagcctg acgacctttt gcgacagccc ctttttcccc ctatggaata atctattttc
      601 gattactctc ggaattctta tattcgaggc accgtttggt cagactccaa ttgacatcta
      661 ttgacattcc tgcccacatg cccttacatt atagatggat catgaacgag cgttcgtgat
      721 tttgaggtca gccgcccgac cagcctatgc aaaacgggcg gttatttttt gtttaaagct
      781 tattcaaaac tttcggatcc cattcaggct gacgagcaat tttatcaaat gaatgatttg
      841 catcaaaaca agttataaat tcagcttcaa tagcttgagt ccttcgattt cgtctgaatt
      901 cgataactac gatgaaagtc tgataacgat atgcaacccg tttataaggt gctcgtgttg
      961 ctttcttgta gtcccatccc tgatggcatt ttgctggatg ttcaagcgtc ggctgaatcc
     1021 aatccatgta tttcgctcta taaaatgaaa atctaccttt tttatctttc ccgtcatctt
     1081 catagaatgc atgcttgaag tgggatggac gaatgtgaac tctgtatcca tcatgggtac
     1141 ggatatctaa tctacagatg gtcctcacaa aatagtcctt gtattcttgc acactggtaa
     1201 aacatcttag ctgaggggtt gttggattat tcattcagat tgatcctcac aacacttcag
     1261 atcaatgaaa aatattgtgc gtttgcacca tctttcctgt aggcccgatt cgataaccaa
     1321 gatgatactc ttaaagcctt caccccgtac gtgtaaagag gcccaaatgt cccatccaac
     1381 cccttttgtt cacctacaac cgaataagca ttacatgaga atccaaacgt caaagcatct
     1441 attgaatcca aggaagggat atccaaatta cggacatttt cgcagatgac agattctgga
     1501 ttgtcaggcg ttatattccg gcgatatgta ctgcatgtat caggatcata atcactggcc
     1561 gaggcatgtt cggttttaaa gccctcaacc gatgccttga gtacaacaca ggctagtcct
     1621 cccggtccgc aaaataattc tcccagcctg aacgtcttga tgtccattaa attaattact
     1681 ccagtcgaga atgttaaaaa ttctataatt caagcttgtt gattttagac gatcaacagt
     1741 ttgtagcaat caacataagt tggatgatta gaccaactca attgtgtcac cagtggtgac
     1801 actatctcgt tgcccttgta aagctcataa aactgcttca ttctataaag accccggcgg
     1861 ttgaatcctt tcaaatcagg atagttcgct gcaaagaaga agtcggcgag cttccgcaca
     1921 aaggcatcat cctattcagc gttttcagag tgtacgttga tgtattcacc tatatcgaga
     1981 tacatcaaaa taagttcttc attcaccttg tgataggcgc gttccctggc ggattcgaca
     2041 atggcaacaa catttttaac atgctcaccg aaccgtatta caccactcat agctgacctc
     2101 cccccactct tttcatcacc tcacgacttc caattcgtga tttgttcgtc gattgcatca
     2161 atgacaaaga cgaaggagcg cgagctaggg cattgatgca tccgattctg cttaacggaa
     2221 gagatccaac ttattgcttt gagttgataa gaatataccc cttcctctcc ttgagccccc
     2281 tcttgcccat gtgataggat gcggtgatga agctgagcta tgagaacaag gaacgtgaag
     2341 cctcgattct ggcgaatacg gaagccgccg tgtttgacgg ggaaatcggt ggagggaacc
     2401 ttctgatcca tggtgacaac ctcaatgtcc ttcaggcgct tcgtgcttcc ttttcagaga
     2461 aggtcgactt gatctacatc gatcccccct actccaccaa tacggtttac cgaattggaa
     2521 cggaacgaac cggaacggtg agcttcagtc cggaggatga catcgcctac cgggacgacc
     2581 ttggcggaag cgaatacctc gaatccctgc gcaagcgcct gatcatcgcc aaaaagctcc
     2641 tttccccctg gggctcgatc tacctccaca tcgatgacaa gatcggccac tacgtgaacg
     2701 tcctgatgga tgagatcttc ggccaagaga acttcagaaa cgacatcacc cggataaaat
     2761 gcaaccccaa gaacttcagc cgccagggat acggaaacat caaagacctg attctcttct
     2821 acagcaacgg acctgatccg atctggaatg aagtcagagt tcctttggat gaagcggatc
     2881 ttaatcggct tttcagaaag gtcgacaagg acggccgccg gtatacgacg attcctctcc
     2941 acgcccccgg cgagaccaga gacggggcga cgggaaagaa gttcaaagga ctcctgcccc
     3001 ccaaaggccg ccactggcgc tcctcgcctg aagtgctcga aaaacttgat gcacaaggcc
     3061 tgatcgaatg gtcgaagaac ggggttcccc gaaagatcat ctacgccgat gagcatatcg
     3121 ggaaaaagct tcaggatatc tgggagttca aagatcccca atatccctcc tatccgaccg
     3181 agaagaacct cgaattgctc aaaacgatcg ttgcagcctc ctcgaatccg gagagcatcg
     3241 tcctggactt ctactgcgga tcgggcaccg ccctgctcgc cgcccggcag ctcggtcgac
     3301 gatggatcgg catcgacgaa tcccttccgg cgattgaaac cgtcgggcag cgccttggac
     3361 ccacctatca actcttgaag caaatcacga ttggataaga tgcaagactt ggcagatgtt
     3421 ctctcccgat gttacacttg atgtatgcat tatcagtatg cgggaggtat tacatgaaac
     3481 ggattcttat catagcggtg ctggctctca tcgtggtttt cccggttgcg gccgcatcga
     3541 cttcgtgggc tgcgggactc aacatcggaa cgggcgccag ccttgccggc cagttccaga
     3601 tggacaagtt caagctcaat gtcaaccttg gatacggctt cgtcaacaag tatgtcgccg
     3661 ctgatgtctt tgcggactat caggtgtacg agttcaacat caacaaggcc cgcttcgggg
     3721 tgaccgcggg tgccggagcg aacctcgcaa tggtgaacaa agcgttcggc ctggcggcga
     3781 tcgtaccggt cggattggtc taccgcctcg agaacaggga ttttcccctt gatttctacc
     3841 tgcgggtaca gcccggcgtt aaaatccttc ctgatctcgg cttccacatc gacgcctata
     3901 tcggagccgt gtggcgcttc cagtaacgca agcgaggatt ctgcgactga gagggtatcg
     3961 gtgcataccc tctttttagt gtcattcatt gtcacttatc aaaacaaaag ccctatttca
     4021 taatcggttt atccccaaag tgagtgtata ctgggagcaa tggaggtttg caatgtgtga
     4081 tacgatcggc cgaatcatgg atgaacaatc ggttctcttc ggaaagaaca gcgaccgtca
     4141 tcccaaggaa gtccaggttc tcgagttcgt caccggaact cttgaatccg accggtatcc
     4201 ggccttggag aaatataccc cccagtttga aaccttggaa aaagcccacc ggcattttga
     4261 gcatccctac cctgccctca tcagccgccc gtcgtggatc tggggcgccg agatgggcgt
     4321 caatgaccgg ggagtcgtca tcggaaacga agcggtcttc accaaacaac gaagcctgaa
     4381 ggaagggttg ctcggcatgg atatgctccg gctcgccctt cacaatgctt ccactgccga
     4441 agccgcggtc gagttcatcc tgaatctgct tgattgctat gggcaaggcg gcgacggagc
     4501 cttcatcggg aagctttcct actccaactc cttcattatc ggcgacaaga accggctctt
     4561 tatcctggag accgcgggaa gtcgatgggc ggtaaagcag atcaagaccc atgatgcgat
     4621 cagcaacgcg tacaggatcg gaacatcgta tgagagaagt gatgccgtca gcagcggcaa
     4681 gcacttcgcc gaaacgtggc gcgacggatt catggagttc ttctccaaag gccgggtacg
     4741 ccagaagacg acgtccaatc tgttggcgga cgccgaaccg acatggatgg gaatgcggga
     4801 tgttctccta tacaacagag gaacgcttgg aaaaatggat cggtcgatga agtcgatcgc
     4861 catcaacgcc tccttcccca agccaacccg gacgaccgct tcgatggtgg tggaatatcc
     4921 tgaaaagaac attctcatct ggtcgtgccc cgcccccctt ccccaatacc atcccttcgt
     4981 tccgatcatc ttcggaatcg acggcatctg ggaagagaag aacagctatg aagatgcgaa
     5041 gaagcgccag gatctcacca acgccctgct gcgaagctcg gcccaggagc gcatcaaggc
     5101 cgcagacgca gcccgaacgc tggacaagcg gttcgaaaag cggatccgaa ccgccttggg
     5161 cgataaggcg aaactcaaaa aggcggtcga gcagtgtacg gccgatttcc acaagcatga
     5221 agaggatctc gccaagaggc ttggaaagtg aaaacaccaa tctaagtctt ttgcatacaa
     5281 gtatcttttt atcgattccc tccacttctt tactcttttt cattcctgga ttatcctaag
     5341 agaaatcttt gttgccgtaa aaacaaatgt tttatactgt taacagatat tagctgacag
     5401 aagaacgctt attcactata atataatgaa gaatactggc ctttgggctg gataatataa
     5461 gaaaggagat ttttacatga acaagaaact gtttattctg gttctggtgg caatcttctc
     5521 cgcgagcctc tttgctatgg ccgcaggaga aacggttgca ccaaaagttg aaacgcaggt
     5581 aatcctcggt tcgtcgaccc aggtcaacgc cgacttcttc agcggctgga ccaacagcgc
     5641 gaccaacagc tacctcaaga cgctgatggg tggatacgag acggtatcct ggacgaagga
     5701 aggccgctac gcgatcaacc ccaccgccgt caagtcctgg aaggccactg acaacgccga
     5761 cggatcgaag acgtacgtgt tcaacatcgc caacaacctc gtgtacaacg acggaacgaa
     5821 aatcaccgcg aaagattatg tcttcaacat tctctatgcg gcttctcccc aggttgttga
     5881 aatcggcgcc cgctacggag tcggccccga gtacgtcggc tatgccgcat accacgacgg
     5941 atctgcaaaa gagttcaaag gaatccgcct ccttggcgat tactccttct cgatccagat
     6001 cgcggcggaa gagcttccct actactatga cctatctctg gtctccgtcg gcccgctccc
     6061 gctgcacgtc atcgcccccg gagtcaccgt gaccgacacc ccgaacggtg ccaagctttc
     6121 cgacaatttc accaccgacc tgctgcgcga gaccgtgctt acgccgaaca ccggctaccg
     6181 ctatcgcccg ctcgtcacca gcggtcctta ccagttcgag agctacgacc ccgccgactc
     6241 caccgcatcg gtcattctca accccaaatt cctgggcgca tatgacggtg ccaagggcaa
     6301 tatctaccgg atcatcatca agctgaccaa caccgcgacg cagatggacg agctgaagac
     6361 cggcgcgatc gacatgctgc agggaatcag cggcggagcg cagatcaccc gcggtctcga
     6421 cctccacgat cagggaatcg tcaacttcgc ttcctatccc cgctacggct atggaaagat
     6481 cgccttcgcc tgtgacttcg gaccgaccca gttcccggca gtccgccgag cgatcgccta
     6541 cagcctcgac cgaaacgagt tcgcccgcca gtacacaggt ggatatggaa tcgtcgtcaa
     6601 cggatactat ggcgcttcga tgtgggaata ccgggagaac aaggatcgcc tggacaaaga
     6661 gctgaacagc tatgcataca acctccagaa ggctgaggac gagctggtca aagacggctg
     6721 gaccctgaac aaggacggca aggcattcgt caagggcgtc gatcagatcc gctacaagaa
     6781 agttgacggc gagctcatgc cgctgtccat cgagtgggcg aacaccgcga acaacccggt
     6841 ctccgacctg atcgcggcga tgctcgttcc tgaagtcaag aagatcggca tgcagatcaa
     6901 cggaaccacc gtcgacttcg ccgtgctcct cgaccacctc tacaaggacg gcatcgaaaa
     6961 gcccgtatac ggcatgttca acctcggaac cggttttgcg accacccagg caatctggta
     7021 ctactactcc accgaccccg aatacttcgg aagctacaac accaacttca tcgccgacaa
     7081 ggagcttgag acgatcgctc ttgacatgaa gaggaccgat cctcaggatc agaagggctg
     7141 ggcggagaag tggatcctct tccagaagcg ctggaacacc ttgatgccgg atattccgct
     7201 ctactcggac atctaccacg acttcttcaa cccgaagatc gtcggatacg attcctcgcc
     7261 gacctggcaa ttccagtacg cgattctccg agccgacgtc aaataagatc gttggatcaa
     7321 tgctttgggg caaccgtttg gttgccccaa gcgtatcttt cccaaaggaa acctacccat
     7381 gttcaagtat gttctcagac gccttatgta tatcgtggtc gtctttctca tcggatcggt
     7441 gctcatcttc agcctctata aatccgtccc cggcgatccg gccaggttga tgatcgaagg
     7501 aaacaaggcg aatatgaagc ccgccgagta tgaagctctt taccgggacg ccgtaaagaa
     7561 gctcgggttg gacaaacccc ttgtcgttca atatttcacg tggcttttca acacccttcg
     7621 cggtgatttc ggctattcat tgacatataa gatgccggtt cgccagatga tcggagttcc
     7681 catgaagaat acgatcatgc tgaacgtatg gtcgttgctc cctgttttcg cgatcaccat
     7741 cccccttggc atcgccaccg ccgtcaggcg aaacactcga ttcgactcca ccgttcaggt
     7801 catcacgatc atcggctaca gcctgccgtt cttcgtgatt gccctgcttt tcatattttt
     7861 atttgccgtg aagctcccga tctttccgat cagcggagtc cagacccccg gtcttcgggg
     7921 cactccgggg agaatgttcc tcgacaagct gtaccatatg gccctgcccc tcctcgtcat
     7981 ggtgttcacc tccctcggcg gactcacccg ctatgtccgg gcgacgatga tcgaggcgct
     8041 cagcatggat tacattcgga ccgcccgggc caaagggttg acggaaaaag tggtcatcta
     8101 ctcccacgcg ttccgaaacg ccctcatccc cttcatcacc atcatgaccg gatggttcat
     8161 ctcgatcttc agcggaagcg tcgtcatcga acggaccttc ctctggaacg ggatgggaaa
     8221 agtgatcatc gacgcgttga atcagcagga cttcgccgtc tcgctcgcga tggtgatgtt
     8281 ctatctcgta ctcagtctcg ttggaaacct tttgatggat ctctcctacg ggttggccga
     8341 ccctcgggtc aaactgaatt aggagtacta tacgatggca aaaaaaccct tgggatcaac
     8401 gtttaccgac gtccttaaaa ccttttttct cggccgccag cgggcgaccc tctccatttt
     8461 ggaggaggag caggtgcaga gcccctatcg gacgatgttc cgaaacttcc gggaaaagtg
     8521 gagcgccatg ctcggtgtct atatgttcct ggcaattttc ctggcggtct tcattctgcc
     8581 gatcttcttc ccgttggata cccaattcca ggatccgacc caacagaata ccagacccac
     8641 cttctcctat atgaagtatg acaaggctct tcagggcaat gtcgcctcga tcgacggagg
     8701 atcggtcttc ggcgtcgggg tcgaccggga tggaaagctt tatcaatggg gaaccttgtc
     8761 ggcgaaactg cagaagatgc cccccaccga caagcgttat gtgcaggctt ccgccggcct
     8821 ggatcacgcc gtcgccctcg atgccgacgg caaggtgcat acctggggaa atgatcggtt
     8881 cacgctgaac atgattcccc ccgagctctc gagccgaaaa gtgaagcagg tcatcgcagg
     8941 ccaccagatc tcgatcgccc tcaccgaggg cggagagatc tacgtgtggg gcaactccaa
     9001 cctgatctcg atccccgttc ctaaagacaa gcagggcacc ttcacgaagg tcgtggcgaa
     9061 cacgaccctc gctgcggcaa tcaccggcaa caacgaactg gtcatcctct cctcccggga
     9121 gacgcccttc tcctatatcc cttccgagat ccaaggccgc gtccgcgatg tcgacctcac
     9181 cgaacgctcc gccgccgcgg ttaccaccga cggccggatt tacgtctggg gcggtaacga
     9241 ttacggtgtg atggacatcc ccgcccatgc ccaaggccgg gcgatcgcag tcagcgccgg
     9301 acggggacac ttcgtcgccc ttctggatga cggaagcgtc gttgcgtggg gcagaaacaa
     9361 ctacaagcag acgaacgttc cgaagttgaa gaatatcgta tcgatccaat cctcgtacta
     9421 tcagaactac gcggtcgaca agaatggccg aatccacacc tggggactga aaggctatct
     9481 gatgggaacc gaccagtatg gccgcgacct cttcgtccgc cttttgaccg gcggtcgggt
     9541 gaccttgacg atcggggcgc tcgctgtcat catcagcgca atcatcggaa tcatcatcgg
     9601 atccttctcc gggtactacg gtggaaaaac cgatatgttc ctgatgcgtc tcgccgaggt
     9661 cgtcaacgcg atccccttcc tccccctggc gatgatcctc tcggcgatta tcggcagcag
     9721 gatcagcgag acgggaagaa tctcgatgat catggtgatt ctgggccttt tgaactggcc
     9781 atccctcgcc cggctggttc gcggacagat cctcgcagcc cgggaacagg agttcgtcac
     9841 cgcggcgaag gcgatgggca tcacggagat gaagatcatc ttcaaacata tcctgcccaa
     9901 cgtcatcacc gtggttcttg tcaacctcac cctgagcttc gccctgtcca tgctctatga
     9961 gtcttcattg agctacctcg gtttcggagt gaacgaaccg aatgcgacgt ggggcaacat
    10021 gctctatgga tgcaacgact cgacggtcat gtcgctgtac tggtggcgct gggtgttccc
    10081 ggcgatcgcc ttgagcttgt gtaccgtcag tatcaatatg gccggagacg gcatgcgcga
    10141 cgcgatcgac ccgaagtcga tcggtagata ggagtactgc tgtgtcgacc ttacttgaat
    10201 taaacgacct gcacacatat ttcaccacga aacgtggaat cgtcaaagcg gtcaacggag
    10261 tttcctacaa agtggaagcg ggaaagacct tgggggtcgt cggcgaaagc ggatgcggta
    10321 aatccgtttc ggcgatgtcg atcctcaagc tcttggatgg caatggatat atcgccggcg
    10381 gctcgatcat cttcaaaggt caggagctta ccaccctcag tgaaaaagag atgtacgagg
    10441 ttcgcggcaa tgcgatcagc gtcatcttcc aggaaccgat gacgagcctg aaccccgtct
    10501 tcaccgttga gcggcagatc agcgaagcgt tcatggtcca ccagaatatg gacaagaaga
    10561 ccgccgcccg ggagtcggtg cggatgctta ccgacgtcca gatccccaac ccggaggagg
    10621 tggtcaagca atatccccac cagctcagtg gagggatgcg ccagcgggtg atgatcgcga
    10681 tggcgttggc gtgcaaaccg gatctgctga tcgccgatga accgaccacc gcgctggacg
    10741 taacgatcca ggcccagatt ctccggttga tgaacaacct tcaaaaagag aaggggacgg
    10801 cgattctctt catcacccac gatctcggag tcatcaacca gatggcggac gacgtggcgg
    10861 tgatgtacag cggtgaagtg gtcgagatgg cgcccgttga cgtcatcttc ggagaaagca
    10921 agcattccca cccctacacg gaaggattga tgctctccgt cccccgcctc gataccgcca
    10981 gccaatcgaa gttggaaacg atcaccggaa gcgtcccgca ccccctcgac cttcccaaag
    11041 gctgtttctt cgccccgcgg tgcaagtacg cgacaaagaa gtgccaaacg gaacacccgg
    11101 agttggtcag tgtaggaaag cagcacaccg ttcgctgctt ctatccggaa aagggggcac
    11161 gaagtggaaa ataagaatgt tctgttcaag atacgcggat tgaagcagca tttcccgctg
    11221 aaaaagcggg ggcttgtcgt tcgggccgtc gatggagtcg atgtcgacat ccttcgggga
    11281 gaaaccctcg gcttggtcgg tgaaagcggc tgtggaaagt cgacctttgg ccgggttctt
    11341 ttgcagctct accggcaaac cgacggaaag accctgtact atggaaggag ccttgaagat
    11401 cttgctcccg cctatgtgaa aaacacgtac actcatttgg cgaaacacaa gaatcagctt
    11461 cagatattgc agaaggcttt cgaagatgca gccgccgaat acgaacggct gaaggatgat
    11521 gaatctcaga aagccgaggt cgtctatacc gcattgaaca aatcgaacga agcggagaaa
    11581 gcgtaccgga acgcctattt ggacatcgtt caactgatcg gggggctctt cgtcgtggaa
    11641 gacgttgaag agatcaaggc tctcctcttg gatacgtacg aagcaagctt cgagcgccac
    11701 cgggtcgatg agcagctcaa agacatcgcc ctcaagatcg ctgaaactca gcgttcacac
    11761 aacaggaagc cggcgagctc caaggagaag cggatcgctt ccctcaaaga gatcgaagcg
    11821 accctgaaga atgctcttga aactgcagcc tccaacattg tcgaggctaa caaaaaactc
    11881 gaacaaaagc ggaaggaata caggcatgtc ccggggtttg acgagcatga gagctaccgg
    11941 gatgagggaa tcgacctgag tcggctcacc ttcaacgaga tgcgccttct gcgcaacgat
    12001 ctgcagctga tctttcagga tccctactcc tccctgaacc ctcgaatgac cgtcggtcag
    12061 atcatcggcg aaggactttt ggcccaccgc ttcttcaaga aagatggtcc ggagatgcag
    12121 gagtatgtga tgaaggtgat ggacgagtgc ggccttgcgc cgtacatgat ccaccgctat
    12181 ccccaccagt tctccggcgg acagcgccag cggatcggaa tcgcccgatc gctcgcgatg
    12241 aatccccagt tcgtcgtctg cgacgaggcg gtaagcgccc tggacgtttc gattcaagcc
    12301 cagatcatca acctccttcg ggacttgaag gagaaatcca acctcaccta tcttttcatc
    12361 agccacgatc tggcggtggt gaagtacatc tccgaccgga tcggcgtgat gtacctcggg
    12421 aacatcgtcg agctcgctcc cgcagagagc atcttcacca atatgatgca tccctatacg
    12481 gaagcgctgc ttgccgccat tccgacgacc gaccggggtg cgaagaagga gccgatcatc
    12541 ctcgaaggtg atattccttc cccgatcaac cctccgtccg ggtgcaagtt ccatacccgc
    12601 tgccgctaca agaccgagat ttgcacgatg gtggagcctg aatggcgcga gttgggcaag
    12661 gggcactttg ttgcctgcca ccacccgctg acggagaaat aggatgctct ttcccaaaaa
    12721 gattcgacgc gtcctggatg tcaggaaagc cgaagagaaa gagctgaccg aagaactgga
    12781 gaaaggcgac atcccggcga tgatcatcgc cgcctttctc gtcttcaccc cggtgattct
    12841 cctcatcatc ggaatcgtat ggggtctcta ctggcttttc acccactgag aaccatcaat
    12901 ttccatcgct ccaagaccgt tcaaatcgtg ctatactgtg ccctacagac agtgatatct
    12961 tctcattcga ccaaggtgca tcttttgtat agtaaaaagg tttttgtgta gcagaaggaa
    13021 ttctctgcag atatcgggaa tataaatcca gatattcccg gaattcatgg agttccttga
    13081 tttccatacg taccggaagc agcagagaaa tgtgatatct taccggtgca gggcgcgtat
    13141 accgggctta atcgcctgaa ccgtatggaa cggcatacta ttgaaggtca agggaaatgg
    13201 gattttaaag ccaaaacata gagtggaagg aatcctggca tgatgagtat ctgaaatgga
    13261 tttgcggctt tgccaatgct caaggcggga aaatatatat tggaaaacgg gacgatggaa
    13321 ccgtcgttgg tattcaaaat gccaagcgct tgcttgatga tctgccaaac aaaatccaaa
    13381 acaaattggg aatcgtggca gacgtcaatt tactcacgga aggcgaacat gaatatcttg
    13441 aaatcgtcgt gaatccttgg tcatttcctg tcaattatga tggtgcgtac tattatcgaa
    13501 gcggcagcac gaaacagctg ttgcagggaa atgcattgac cggttttctc atggaaaaaa
    13561 caggacgtaa atgggatgcg gtgacgatta ccaatatcgg tgtagaggat ttggataggg
    13621 aaagctttga tatcttcagg cgcgaagcgc tccgcagcgg tcgcatgaca aaagaagatg
    13681 tagatatatc aaatgccgag cttttggaaa agttggatct gatggcagat ggaaagctca
    13741 aacgtgccgc ggccttgtgt ttttaccgtc agccggagaa agtcagcagt ggctgttatg
    13801 tcaagatcgg caaatttgaa ggaagtgaga ttgtatatca ggatgaagtg cacggctccc
    13861 tgttgatcat ggccgatcgc atcatcgatt tgatgtatct gaagtatctg aaagctgcaa
    13921 tctcttatca taaagaaacc agagtggaga cctatccttt tgcacgggaa gccgttaggg
    13981 aggcagtttt caacgccttg attcattgta attgggcaga taatgttcct gtacaaatcc
    14041 ggattgaaga agatgtgatg tatgtgagta attgcagcat gcttccgttt ggctggaccg
    14101 tggagacact tttggactca catacttcaa agccgtacaa tcccgatatt gctcgtgtgt
    14161 tctacagagc gggttatatc gaaaactggg gacgtggaat acagaaaata cgtgatgctt
    14221 gcaaggcgca tggcgcagaa gagccggaat ttatcgtcca cggcggggat atcatggtca
    14281 agttcaaggc tctccaaagt gccattgtga cagattccaa agtgtcaaat gtcacaaagg
    14341 atgtcacaaa ggaagtccca atggaagaag caatcttatg tttgattaga gataataaag
    14401 atattacaac gactgaaatg gctgagtatt tggcagtaaa tagacgaacc atccaaagag
    14461 ttttagatga cttgaaaaac aaggatattg tcaaacgtaa aggcggtaaa cgttatggtc
    14521 attgggaaat tcttgatcag tagaggtaac tcaattgctt cacattgtgc accggtcgct
    14581 tttcccaatt gtaatcggtc aatgtgtctt ctcataactg ggaaaataat cttcaaagat
    14641 cgtcatgaaa ctaaaaacat agagcaatgt aagcaatgag tagcatttgt tgcaggaatc
    14701 ggatgaaaat ccctataata atgattttag cgtaaatttt gttttatata ttcatattta
    14761 cgtattatca ttgcattcta acaattatta cagcataata atgattatta cggagacagg
    14821 ccgatgaata acgattcaat ctattccgga cgatatgtgc ttccggcacc gggaacaccg
    14881 gccggctaca cggccctgat caatcactat cacctgaaga ctccgtatcc ttcgctcaag
    14941 atggcaatcg gggaagggta tgcccaacaa gaccatgatg gatggcgatt tctctccaag
    15001 cgtgttcgga ttgaacctac cctcggaagc cagctcacct ttgcccttag atatgaaggg
    15061 atcaatcttc tggttctcaa gaagctcttt gaagcggttc ctgttcaaga gattgaagat
    15121 gtcgtcaaag agtccatcac tggccaatac gcccgtcgaa tctggtttct ctatgagtgg
    15181 ttgatggatc gcaggctctc attggatgac gtgacccaag gcaactacat cccggttctc
    15241 gatccgaaac aacaatatgc ggtcgccggt atccgctcgc cgcggcatcg agtgatcgac
    15301 aatcttccgg gaactcccgc tttttgtccg cttgtaacaa aaacacccga gctgctggaa
    15361 tttgaatcgc ttgccttgca agatcgtgcc tcgactctcg tatcgacgat accaaaggag
    15421 attctatcaa gagcagccgc atttcttttg ttaaaagatt cacgagccag ttttacgatc
    15481 gaacatgaat ctcccacccc ggatcgactt gcacgatggg cgacggccat cgggcaggcg
    15541 ggtcgtgttt cactgagtaa agaagagctc cttcggcttc aggagcttgt catcggagaa
    15601 tcggttttca tcaagccggg tttccgaacc gaaggcgggt ttgtcggaga acatgaaccg
    15661 atgacgggat tcccgatcgt cgaacatgcg agcagccgaa gtgaagacgt gattccttta
    15721 gtggagggca ttcttgcata tgaacaacgg acccgagatt cctttgaccc cgtcgtgagt
    15781 gctgcaagcg tggcattcgg gtttgtccag gttcacccgt tcagcgacgg caacggaagg
    15841 attcatcgct atctgattca ccatatcctg gctcagcgag gcttcaatcc acccggaatc
    15901 atctttcccg tctcccatgc gatgctgaaa cgggcacagg agtatcagcg ggttctacga
    15961 gcctactcct cctcgattct gccgttcatc gagtggaccg tcacccccga ttacaacatt
    16021 cacgttctca ccgaaaccgc cgactactac cgctacttcg atgccaccga atacactctc
    16081 ttcctctatc ggtgtgtgca ggatacgatc gaggatggtt ttgagcaaga agtatcgcac
    16141 atcatcgcct atgaccgctt tcaagcaggt cttcagcgcc ttggcgagat gccggatcgg
    16201 agcgttcagc tcctctacca attcttgaga caacacaacg gaacgctttc aaagcgggcc
    16261 gaagggaagg agttcaaaga gctttccgtt cagatgatta aggaaataga agcgatctat
    16321 gccgaagcgt tcggaaccgg ctcttccttg gaatgaagat gtggtaggat ggtccataaa
    16381 gaaaagattc ttcctggagc atgaatgatg aacgttctta tcaccggagc gacctccggc
    16441 atcggctttg aaaccgccct tctctttgca acgaatggag ctcacgtctg gggagcgagc
    16501 cggaatccca tcgcctctcc cagcaatcgg atcgtcttca gacggatgga tgtcacaaac
    16561 gaggagtcgg taaagaaaag cgtcgaagag atctggaacg aagcggtgaa gacgacgggg
    16621 aaaggaatcg acgtcgtcgt ccactccgcc ggatatggaa tcggcggcag cgccgaggac
    16681 accccgattg atgatgcaat cgcccagttc gacaccaact acttcggagt cctccgcgtc
    16741 aaccgggttc tccttccccg catgcgggcg cagggaagcg gcagggtcgt catcctcggt
    16801 tcgatcgcgg gacgaatcag catccccttt caaagccatt acagcgccac gaagttcgcc
    16861 ctggaagcgt acaccgaagc gttgcggctt gaaggcgcgc cattcggcct taccgccacg
    16921 attatcgagg cgggtgatac gaacaccgca ttcactgcca acagaaggct gacgatcgat
    16981 ccatcatccc cctaccatga tcaggcgctc cgttcgatcg ccaggatggc ccgcgatgag
    17041 gaactcggat atcccccaac caaagtagcc aagatgatct ataaggtgtc acaaatgaaa
    17101 aacccgccga tccgaaaacc ggtgggcctt tcatattccc tcctgatgct gctgaagcgg
    17161 cttctccctg attcccttgc cgagagaatc atcaaagcca tgtatatgcg ttagtcatgc
    17221 ttggaattgg cgtcaatgag aacgaccacg atcaggatca atcccttgac gacccattgc
    17281 caatagctgt tgatgctgag cagaaccatc acgttggaaa taacctgaag gaacagtgct
    17341 ccgatgatga cgccgaacac cttgccccgg cctccggtca gggatgttcc gccgagcacg
    17401 acgatcgtca tcacgtcgaa cagcaggttt tcaccgatgc tcacgtttcc gctgagggtc
    17461 ttgctcgtca gcccgagagc tccgatgcat gaaagaaccg cacagatgac aaacgccagg
    17521 attccgatga agttcgtgtt gatccccgac agcttgctcg ccacccggtt tccgccgacc
    17581 gcatagaagc ttcggcccaa ttcagtatgt tgcgtgaaga catagagaat gatcgcgacg
    17641 atgagcaaca gaatcaccgg gaacggaacg aatccgacat acccggctcc gaagaagttg
    17701 aacgcatcag gcagccccga tatgggcaat ccccgagaat atatgaagta gattcccttg
    17761 atgatgttca tgaccgtcaa cgttccgatg aaggaaggca tgttgccata gatggtgagc
    17821 acgccgttga ctatcccgac gaagatcgaa agggcgaaca cgatgacgat cgcccagaac
    17881 atcggaatcc cggcgtttac gaccattccg gcgaaaaaga tcgacgtcat gccgatgatc
    17941 gaggtgatcg acatatccat gtgcccgatg aggatcacaa agaactcacc caaggcgatg
    18001 atcgagatga acgacgcctg tcggaagatg ttcatgatgt tgttggtggt tctgaacttt
    18061 ggaagggcga tgctgcagat gataaacaag gcgatgaaca ggaagagaat catccattct
    18121 ttaaaaaagg ccttgtaatc ctttttaaac aacctttcat cgccgccaag tacttttgtg
    18181 ctcatgcttc tttcactcct aatagatctt ggatgatcac ttcctgattg atatcgtcgc
    18241 cttcaacagt atccatcact ttcccctcat acatcacata caccttgttg gaaagtccga
    18301 tgatttcttc cagttccggt gaaaccaata cgattccttt ccccgctttc gccagttcgt
    18361 tgatcaatga atagatttca aacttcgctc cgatatcgat gccccgggtc ggttcatcca
    18421 gcaggaacag gtcgcagttg cgcagcatcc atttggcgat gacgactttc tgctggtttc
    18481 cgcccgaaag cttgctgatc aattggctga ggctcacata gcggagcttc aacttgtcgt
    18541 tgatgtcaaa agccgctttc tcctccgccc gatggttctg gaagatcttc cacttcttga
    18601 acatgaacat cgacggaagc gtcgtgttgt cctgaatatt gaacagcatg ttcaaaccga
    18661 gcagccgccg ctcatcggga atcactccga gccccagttt gatcgcatgc tgcggagact
    18721 tgatctccac ctgtttcccc cgtacgaaga cgtcgccgga tgttgtttta tagtttccgt
    18781 agatcgcatg gatgagctcg gtcttgccgg cgcccagcaa tccggaaatc ccgacgacct
    18841 ctcctgcccg gacggacagt gagacatcgc ggaacgatcc gtcatgagcg gtgaggtttt
    18901 tcacttccag gagtgtttca ccgaactcct cattctttgg cggatataca ttcttcagct
    18961 cgcggccgat gatcatcttg atcaattcgg gtttgtcgat gtctttcaca tatccgctgt
    19021 tgatccattt cccgtcacag agaacggtgt accggtcgca gcactcgaag atctcatcga
    19081 ggcggtggga gatgtagatg attcctatcc ccttctcctt caaaagacgg atgatccgga
    19141 agatgttctc gatcccgtcc tcctgcagca ccgaagtcag ctcgtcgaag atgatcacct
    19201 tcggattgtc cagcatcgcc ttggccagct ggatgatctg cttttcggct gtccggaggt
    19261 gtttgacctt caccttcaga tccacgtcgc actgaagata ttccaagagc gcctcgccct
    19321 ccttctgcag ctccttccag tcgatgagcg gacctcgttt ggtatatctt ccgatgaaga
    19381 tattctccag accgttgagg tcccccatca gctgaaactc ttggtggacg atcgcgatcc
    19441 ccatctcctg agactttcga acgttgaagg agggaatatg gattttattc ccttggatgt
    19501 acaactctcc ctccgtcatt cgattgtcac cgatgatgca cttcgaaagc gtcgattttc
    19561 cggctccgtt ctcaccgaca agcccgtggc attcccctgc gagcagcgta aagtccacat
    19621 tgtccaacgc cttgactccg gggaacacca tcgtacagtt ttttacttct accaacgtct
    19681 ccacattgac cgcctttgcc tctacaacca tagccaaccg agagcctcaa ttcccgaaca
    19741 gatgatgtcg ttgtcgggat tctccgggtt cctgatgttg tgaatcaacc gcttgatctc
    19801 gtgatacggg aacatcgagc tcttgacccc ggtctctccg accggattga tgtcgaagaa
    19861 catctccttg gcgatggcga aatcctcctc gttgtaggcg agaatctcat ccaggatccg
    19921 atcttttttc ttcggatcct tctcgtcctc atacctgagc gaagccttga tcatctcgta
    19981 gatcaccatg ttgcgccgct cggcgttctt cagcttcgca aagtgcggcg cccagtggcg
    20041 gagcttcgga tcggccagac acgccttgtg gaggttggtg agcttttcaa agatcttggg
    20101 atgacgggct ttcgccgctt caatctcggg gatgtcgctg tttcggatct tgttcttctg
    20161 gaacgggaac ggcgtcgtat acagcggcat ctggctctgg tggatcgtga gcatcgtccg
    20221 catcacatag aggacatcct tgccgaggtc gccgaagttg agctcgcacg ccttgtcgaa
    20281 gaactcatcc atctccattc ttgaacccca cccgtagttt ccgtgggcga acaggtgcag
    20341 gaacagcccg taccactcga acatgtatcc gttgattcca tggacgccca tggcggtgct
    20401 gccgaggtgc tggcggatat ccgtctcgat cacctgctca ttcgtttcat cctcataccg
    20461 gatcagattc gaagcgaagg ttcggtagag ccgccccatc gtggacgtga ttgagttcgc
    20521 ctcggtgtcg cgggcccaga tatgctcctt cccgaacgca ttgacccatt tttcaaactc
    20581 gctttccggg acatagagcc cgggcgccca gaagaggttg atacctttcg gcatctcatc
    20641 gacgcaggaa gcgaggaacg ccggatcctt ttccagcggc ggctgccgga acgcccggat
    20701 gccgagaacc gcgtccttgt tcaccgattt cacccgatcc cagatctggt tgagcagcca
    20761 gatgtttgct ttatgcttcg cttccaccgg ggcttcactt gtttcacccc agcgggttct
    20821 gcagccatcg cactcgcaga accagaatcc ctcttcactt tcttccagcg tgaacccgtc
    20881 aaagccgatc tcggcgatcc gctgcatcga atcgaggatg tagttcacgt tgtcttcatt
    20941 gctcaggcac agggaatcga aatcgttcag gaactggcct cccttcggcg ggatcatccg
    21001 ggcttcggga tgcttgatcg aatacgcccc gttgtaggag ttcagcccga cgtaggcgaa
    21061 gaaagaaaac tccatcatat ggcccagatc gaacaacttg gtcagatagg gatgctcgat
    21121 gttcgggtgc gtgtaccgga tggtgagcca gtcatcgttc tcggcgttcc agatatccac
    21181 cgggacgttc cgatagaccg tctcggggta tttctcaaac tcgaacggcc aatatccata
    21241 cataaccatg ttgaactggt tgatcttgtt gtccatgcaa acgttgagga attcgaccca
    21301 cttgtcgtat ccccacagct gggaatcaaa gccgatccgc ccgtagccgg cataccacga
    21361 aaaggtgggt gcaaccgccc gaacggcgac ggaaggccaa tcctcgattt cacaacaggg
    21421 cagggtgtac ccgtttttcc cttgccgtaa aagcattttt attgaagtca gagcgttgat
    21481 gtaccccgcc cgtccttcat aagagagggt gatcccctcc ttgttgatct ccagagcata
    21541 tccctgggat cggagttccg gcttttcact gtgcttcttg ttctcaagcg acggaatcaa
    21601 tgtaacaact atctcatcgt tgccaggatc gtcaaacagt tcggggtaat tccaaaaacg
    21661 ggtaacagcc aacgtaagaa gatccgagtc ggtggtatca attcgaatct tgcggaagga
    21721 ttttgtttct ttcttattat ccacaatgta ttttggttct ggtaacaatg taatcatact
    21781 tcacctgtct ttccattaaa aatcgggtgg agcaggggat gaatccccta ccccacatct
    21841 tcaccgtcct acagccagga ccagttcttc caatcatact gggcgatgtt cgccggtgtg
    21901 atcgcttccg gtttgggaag atcccaaacc ttgtcgaggg tcttcttctc gatcagggcc
    21961 tgatacatcg ccttcgtcgc ctgatacccg aggtctaccg ggttctgcag gacttcgccg
    22021 agccatgctt cgttgccgct gtcgatgtag cccaacacgt ccttgtctcc ggcgatgccg
    22081 atgaacttga tctccttccg gttggccgcc cgggcagcgt tgtagggacc gactacttcg
    22141 gcgtcattgt gcccgctgac gacatcgatg accggctcgc gctgcaggac gttttccatc
    22201 aggctctgtg aggagtcccg ggaaccggcg ccgacgtcac cttcttcgat gatccgggct
    22261 ttcgggaagg aagcgagaac aacgtccctg aatccctcga accgatcgac cgagacctgt
    22321 cccgaggtgg gtcttgtaac gaacagacag acgggatcgg gcttgccgag tttcttaagg
    22381 tactcaacgg ccacttctcc ggcggtctcg ccgagcattc tcatatcgtg tccgacaaag
    22441 gtctcccaga cgatgtcatt tgggctcttc ggatcttgag catagtcgcc gatgacgaac
    22501 ggaatacctt gactgacgta ctgcttcatc gtcggaatcg ccgaggatgc gtccgccggg
    22561 aacaggatga ttccttttgc tccaccggtg atcagggcat cgatgttgtt cagcatcgtc
    22621 gatacgtccg agcgggagtc ctcgaattgg aaggtaaagt ttgcatccgg attatcggca
    22681 gcccactgct tacaaccatc aatgaccccg aggtaccatg tatacgctga aagctccatg
    22741 atgctcactc cgatttttgt ctccttggca gcctcttccg cccgcccggc accgaacagc
    22801 atcgagccga ccaagagaaa aatcatcaca aaaaccattc cttttttcat actttttctc
    22861 cttatatggt ttttatttct tcactactcc tttgtggaaa aggaataatg ttacacaatc
    22921 gcttcccgca gccaatccag cacggaattg accatgtgct tgccgatttc cgccgtggct
    22981 tcgacggcgc tttgggcgaa ccattcggtg ttgagcttgc agcgcgtgag gtcgacatgg
    23041 tcgggatagg ttcccatcat cagcgacgtc tcccactttc ccgcatggtc aaaaccgccg
    23101 caggcgatct gggcttcctt gctgatgagg ggtatcactt ttatgtatga gaagggatca
    23161 tcccccgagc cgaggttttc ataatagtcc tgatagtcgt tgctccccca ccagcccact
    23221 cctttcgtcg cctccatata ctccatcgtg actttcttcg ccgccttgtg gcaggagatc
    23281 gtcatgggca tcaacgcccc ggcttccgtc tggtggtggg gaatgagata gatgtttttc
    23341 acgccgcccc agataagcga cttgaggatg caatacacat attgggtgtg ggtgtcttca
    23401 tcgacgtgaa tcgtgccggt ctcgggacct cccaccgcat aggaagcgac cccgtaccag
    23461 atcggcggca gcacgacgat ctctttttct tttccaagct cccgcagaca tccggtaacg
    23521 accatcgtat cggttccgct ggaaacatgg tgagcgtggt attcaatcgt cccgacggga
    23581 atgatgaagg ggactcgacg ttgttttgca tcttcgatct gatcaaagta catgtcaatt
    23641 aaatccattc ccacctcctt ctctttcgca gcacaaatct ggtattccgg taagcacgta
    23701 caaatcaaca aactccgtac gtgtaattgt acgaccacaa gattaagaaa tcaagaaaaa
    23761 cattacgttt aacttgaact ataatttaaa cgtttaagct tttactctga ttcttcttga
    23821 catcatcggc aggatagtga attgtttatc ttgtatggag aaaaatacat gctgcagaaa
    23881 agaataaacg ttggattagt catcagcctt gccggatttg cgatatcggt gaatacgctg
    23941 ccgccgttga tcgagtggtt ctcgggccac ctctgcatgc cgatctcttc gtttggattg
    24001 gtgtattttt ttcagtatgg cgccttcacc ctctgctcga tcgggatcgg aaagcttcac
    24061 gccaagcggg ggcttccact cctgtccatc gcgatcggag cgctcttctt ttccgctctt
    24121 tgcgtgtttc tgatcggcag agcgaccagt ttcgtccttc tcatcacttt gatgatgctc
    24181 ataggcggtg ccggcggctt ggtggagtcg atcggaacga cgctgctcac cgttgaggac
    24241 ggatcgaaca gaatgcttca tgcgtcccag cttttttatg cgatcggcgc ggtggtcgcc
    24301 cccgcgatgg tggggtttct tctggcatcc ggcgtgcatg tgccgaaaat cagccaatat
    24361 atcggaatct tttcgttgct gattgccata gtcgtgttcg gcctgactcg ttcttcgaac
    24421 agcaagacat ccaaggaaac accaaccccc tcgcatcgcc ccgccacagc cccgcttttt
    24481 gccctgtatg tactctttct cacgatgggc gcctacgtca tcgtcgaaat gtccttggga
    24541 aactggcttg cggtgttcgc catccagacc ttctcggtca ccgaagccca aggatcgctc
    24601 gccctctcgc tcttctggac ggggctttgc ctctctcgat tgttttatgt cttgaagagc
    24661 gtcaaatccc aacaaaaaac gctgagcatc cacatggcgc tcatgctcct ttcatgcctg
    24721 atcctcatcg catcgacgaa aatcgggacg ctgaccctcc tctacccgac ggtcattctc
    24781 tttggcatcg ggtgcggacc gatttggccg ctgctcatcg agttctgttc ggcggccttc
    24841 agagaagagc accttgtgat gtatctggtc gcctcgggaa gcatcggagc gctcctcggt
    24901 ccgattctca catcgaccct gttcggcttc tttggcatcc ggaatatccc gatgattctc
    24961 agcgcctata cggtgcttat gatgggctcc ggcgtcttga cgttccggat cttgaggaac
    25021 gctaggcgca cccgcaggag cgccgcttga tgatcgtgcc ttgaatggtg atccgctcaa
    25081 ccgggggaga gtcttcattt gacagtgttg caagcaggag ttttgccgcc tgctccccca
    25141 cggagtgtcc ggtgctgctt aacgtagtga tgggcggttc gagcgattcc atgaagctga
    25201 ggttgtcaaa actgaccagc gccatctctt cgggaatcct caccttggcc tgaatgagcg
    25261 ccttgtagca cccgagggcc atgatcgagt ttgagatgaa cacggcccgg ggacgttcgc
    25321 ttctcggcag accgagcaat tgattcatcg cccggtaccc cccttcgctc gtccaatcct
    25381 ccacgatgat gtgtgaagcg ggcgcctcca ggcgattgtc ctccatcgcc ttgaggaatc
    25441 cttccaaccg ctcggacccg gaactctgat gctgcagtcc ggcaacgcac ccgatcgaac
    25501 gatagttgtg atcgatcagg tgccgggtga gcacatacgc tccgttgatg ttgtcgtgca
    25561 gcacgacgtt cgtcgccacc tgcggcggtt cattgtcgaa caccacaagc ggcatctcct
    25621 ctttcaacag ctcgttgatg gcgtcggtcg tggtcccgac cgatgcaagc aggatcccgt
    25681 ccacgtattt cgaagacagc agctcgagcg cccggacctc gccctcgtgg gagttgccgc
    25741 tgaaggaaac gatcaggctg taggattggg cgcgcagcgc ggcctcgatc gcaaggccca
    25801 cttcaccgaa aaactcgttg gtgaaatccg gaacgacaag gccgatcgtc cgggtccgcc
    25861 gggtccgaag cgtcgccgcc atcgggttcg ggctgtagtt gtatttgttt acgaccttca
    25921 tgatcgcctg ccgggtcttg gggctcacgt ccccctgatt attgacgacc cgtgaaacgg
    25981 tcttgatgga aaatcccgtt tccctggcga tatcgaccaa tcggaccttg ttgttcttct
    26041 tggtgctcat tccgtcattc cactccttga gagcgcggtg gcgatatcct tctcgatatc
    26101 gccgtctccg actccgaagg cgtaccagag ggtaatgctc tgttttccgg gcaagacctt
    26161 gccattgccc atcttcttct cattggtgaa gccggtgggc ccgctggtcg caggaagcgc
    26221 gaaccctgca gctttccggt cgggggtctg cgtcagccag atgaccgtat gatcgagaat
    26281 atcggtgcgg gcgaccaccc aggtggaacc gcctttctca tcgctcatcc ggctgagggc
    26341 gaacttcccg ttcctgaacg tggaaggaga ttttttgccg tagtcgatga ccgcaaccag
    26401 ctcgggatcg agcgtgcaac ctttatcgag ggtcaaaaac agttcgggtt tcttcgtcac
    26461 cccttcgaac gtctcatcaa gcacgacgac ttccctgtcg gtgaagttgg ctatcgaata
    26521 ctcgaggcgt ccgcttttcg gatagttgaa gttgacgtga ccgagatact gatactcaaa
    26581 cggagcgtgg gcaaggttgg tcaacccgct ttcaacgacg acgaacgttc cgcttttgtg
    26641 aatcctcagt gcaggcacga aggaatactc ccctacaaac ggaatgtgcg agtggaactc
    26701 ggcgcacagg gcgagcggaa actccttgtc ctcttcatcg atctcgatcc aggcgcgcac
    26761 cggctgggaa acgggaagct ctccatggtg ggggtgagta tcttccatgg acggatgccc
    26821 cattccgcgg actccgcagt ggatcagaaa cgcaccgtag ttcgccagaa acggtgcttg
    26881 atagaccggt tcttttctga acccctcgaa cttccgggaa actccatcca cgctccagtc
    26941 ccacagctcg ctgcccatga agggggtcca tgtgaacact ccccgcccga cttctatcgt
    27001 aacggcttcc actccgcttt gataggcaaa gccttcaatg gaccaggcgc ggctttggag
    27061 gatggtttgc ttcacaggtc caaagaaccg tttgttcaga ttcaacatcg ttgggttgtt
    27121 tttcttcatg gggcgactcc ttacaggttc cgttcatgta catcacatga tgacaagcat
    27181 gatagtaaac gttgtcaata ttttcttttc actaccaatt tactagtata atagcacaag
    27241 catattgata tttaaacaaa tatatcccca tctttaccga agtatttgaa ttttaccctc
    27301 aggtcaacgt gctgtttata taatgaaaga caacgtttac atttttaaat accgcatccc
    27361 tgttatacac ataactactt attgtaatga tagtaatagg gtctttatgc aaatccggcc
    27421 tgaatattat tttctttgac atatggtttt ccgcatgata aactgacaac gttagcgtat
    27481 aaagttgcaa gaatagccat tctacgcact atgggaggtg cttgaaaagg gaggatcgat
    27541 gaaacgtgaa ttattggtaa tgaagaatat cgataagtcc tttccgggag tccatgcatt
    27601 ggatcacgtg aactttgacc ttcgctccgg ggaagtccat gccctcttgg gagaaaacgg
    27661 cgcaggaaaa tccacgctga tgaaaatcct caccggggtg taccagatgg atggcggaga
    27721 gattgaggta gccggagaac ccgtaacgat cacgaacgcc ggcgatgccc agaagcttgg
    27781 aatcagcatc atctatcagg aattcaacct cttcccgcat ctttcggtag ctgaaaacct
    27841 gtttatcaag cgggaaccca gaaaatacgg aaaatggatt ctcgacggta aaaagcttca
    27901 tgagcaaagc gaggagctgc ttcagcggat ccagttgaat ctcgatcccg cacgcccggt
    27961 cagttcgctc agcgtggctc aacagcagat ggttgaaata gcgaaagcgc tctccctcaa
    28021 tgcgaaaatc atgatcatgg acgaacccac ctccgcattg accgaccatg agatcgagaa
    28081 cctcttttct ctcatcctcc aactgaaaaa gcagaatgtc gggatcgtct atatctccca
    28141 ccgattggag gaactctccc atatcgccga ccgggtgacg gttctgcggg acggcaagta
    28201 cgtcggaacc gtcaactaca gcgacacgac gacccgggag ctcgtcagca tgatggtagg
    28261 gcgggagcta agcgaatatt ttccacagcg ggaacacgcc tatggcgctc ccatcctgga
    28321 agtggaaggg ctcagccgaa agggcgcggt ggacgatgtg agcttcaccc ttcatgaagg
    28381 agagattctc ggagtatccg gcctcatggg tgcaggaaga accgagatgg cccgcctcct
    28441 cttcggcgct gaccgcaagg atggcggcac ggtgaccttc aagggggagc agatcgagat
    28501 ctcgtctccc ggcgacgcca tcaaaagagg gatcgggtac ctcaccgaag accgcaagcg
    28561 cgatggcctc accctcggat tgaacgtcaa ggacaacatc accatggctt caatcaagga
    28621 gtacgcctcg atgctcggcg tcatgaatga ggggaaaatc gaaaaggcaa cctcggagta
    28681 cgtcgatcaa ttgagcatca aaacaccgag tctggagcag cttctgcgaa acctgagtgg
    28741 aggcaatcag cagaaggtca ttctcgcccg ctggttgtgc aaaggttcaa agattctcat
    28801 ttttgacgag cctacccgcg gtatcgacgt cggtgcaaag caggaaatct acgagttgat
    28861 gaactccctc gtcaaagagg ggcattcaat catcatgatc tcctccgaat tgccggaagt
    28921 gcttggaatg agcgaccgga ttctggtaat gcacgaagga agagtggccg ggattttgga
    28981 cggaccaagt gcgaccgaag aatcgatcat gtatctggcc accggtggaa aataaggaaa
    29041 aaggaagaaa cagaagatga agaaattgga actgcgtgcc tcgacacttc agcaattcgc
    29101 cgctctcgga agtttaatac tgatgatgat cctcttcagt attctatcac cctatttcct
    29161 taatttcgac aatctgatca cggtcatcaa gcaaacctcc gtcgtcggca tcatcgcaat
    29221 cggggtcacc ttggtcattg ttaccggagg aatcgacctc tcgctcggct cggtcgtggc
    29281 cctttcgggg gtggtcgccg gattcgctct cgccaaagga gcttcaaact tcgtcggcat
    29341 tctgatcggt gccttgatcg gactgctttg cggtctcatc tcgggggtca tcgtagcgta
    29401 tctggaactt cccgcgttca ttgcgaccct cggaatgatg atgagcgccc ggggaatgtc
    29461 cctggtcctt acccaaggcc ggcccgtata tattccaaac aacaagacct tcaacctgat
    29521 tcacaactac aacctgttca aatacatccc tttgccggtc atctacttcg cggtgatcgc
    29581 gctcctcacc cattttttgc tgaaacggac atcgaccggc cgctacctgt tcgccaccgg
    29641 cagcaacgag gaagctgccc gcctgtcggg aatcaacgtc cggctcgtca aattgttctc
    29701 gtatggtttc tgcggcttca tgtgcggcct ttcaggagtg atccttcttg cccgcctcaa
    29761 ctccgctcaa ccggcgatcg gctccgggta tgagcttgat gcgatcgccg cggtggtgat
    29821 cgggggaacc agcctgtcgg gaggcgtcgg ccagatctcg ggaacgatca tcggtgttct
    29881 gatcatgagc acgatgaaga atggcttgaa cctgatgcac gtttcacagt tctggcagca
    29941 agtcatcatc ggcggtgtcg tcattcttgc tgtatacatc gatataaaac ggcgcgaagc
    30001 acagcgcctg taacgtttga atgctcacgt ggtgggtaat aaaaaattgg aggaaacaca
    30061 tgaaaagaat cgtcacactc gtattgatcg tggcccttct cgttccgacc gttctctttg
    30121 ctgccggtgc taaggaggcg gcggccaaag agacatacat cgccgtcatc agcatggggt
    30181 acatgcatga gtactggcaa gcggttcgtc ttggaaccga aaatgcagca aaggagctgg
    30241 gcatccgaac cacctatgaa gctccgccat ccgaagatat ggtcgacttg cagatcaaca
    30301 tggttgaaaa cgcaattcag accaaacccg acggcttgtt gctcgcaccc cttgatgcgg
    30361 aggctctggt cccctacatc gacaaagcgg tcaaggccgg aatcccggtg gttctctttg
    30421 acgccggagc aaacaccgac accttcgcct cgcttgtcgc gaccaacaac gaaaatgccg
    30481 gcgccatcgc cgcccagaac atgatcaggc tgcttggaga caagggcacc atcggattga
    30541 tcgtccacag cgcaaccgac caatcggcga tcaagcgccg ggacggcttt ttgaactata
    30601 tgaaagccaa ctccaagatc aacgttcttc cccccgtcta cagcggtgga gagcacgagg
    30661 aatccgccaa cctgatggtc gacctccttc gggcgaaccc ggatatcgcc ggtgtctacg
    30721 ccacgaatga aggcgccgcc gtcggtgccg gaatcggtgc caaggaagcc ggcaaggccg
    30781 gaaaagtcac gatcgtcggc ttcgactccg gagcgcagca gatctccttc cttgaggacg
    30841 gaacgatcgc cggctttgtt tcccaggatc cggtaaccat cggatatctt ggtgtaaaaa
    30901 ccctttacga tgtgcttcaa ggcaagccgg tcgaggttga gatcgatacc ggagcggtat
    30961 tcgtcgacct ctccaacctg cacgatgacc acgtccaaca gatcatttat cagtaagctg
    31021 taatcgtctt tcgtggagga ttgagcctcg cccgatcctc cacaccataa aactaccaag
    31081 gagtacactt tgaaaaaacg cgtaacaatc ccggaacatg aatacttcga aagaattgca
    31141 aaagccgcca aactcaccgg tgaggcggga cttgacatcc tgatctccaa ctgcaccgaa
    31201 gcggattatt cattcgttcg ctatttcacg aacttctggc ctctgttcga gacagccggc
    31261 gtcgcaatcg cccccaatgg gaaagctgct ttgatggtgg gaccggaaag caccaaattc
    31321 gccggagacc gaagtgtcct caagacaatc tttaccctcc tcgaatatcg cgagtcagcc
    31381 gacccggcct atcccgagtt caaaccccag accttcaagg atgtcttcaa gcaccttggc
    31441 gtcaccggca agaaactgag gatcggaatt gcagggtatc ttttgacgaa ccccatccag
    31501 ctcgagggat tgcgatcgaa cttccccgat gcggaaatcg tccgggcgga tgacatcgtc
    31561 cggcagcttc gggtcatcaa gagcgagaat gagctcgcct gcatgcgcga ggggttcaga
    31621 atcgttgaac ttgcgaccaa agaggttctc aaggcgatcc gcccgggcgt gaccgagctt
    31681 cagatggtcg gcgtcgccca gaagacgatc tatgagcacg gggccgagta tgaaggactg
    31741 ccgatgtacg tgttcagcga gaagtcgacc agccacgcca tcagccgttc cacctaccgc
    31801 gaaatcggca agggcgacct tgtccagctg aacctctccg ccaagatcga aggatacagc
    31861 ccgagcgtcg ggatgccgat cagcatggga ccccttgtag gcgagaagcg ggagttggtc
    31921 gaatttggcc ttgatatgca ccgatggacc tacaaacagc tgaaggccgg cgtcgaagcc
    31981 tcttcaatcg ctcaagggta ctatcagcgc ttcatcgact tgggcagaaa ggacaacttc
    32041 gtctatggcc catgccatgg aacggggatg atcgaagtcg aggcgccctg gatggaatcc
    32101 tcttcgacgt acaaactgca gccgaacatg accttccagg tcgatacgtt cgcatcggga
    32161 ccttcctacg gacttcgctg ggagaccgga gtcgcgatca ccgaagacgg gtatgatctg
    32221 ctctccaacc cgatcggcga gatttatgag atcgattgtt aaacattgat cgacaaccga
    32281 aaagggggga tttcacagat tcgtgaaatc ccctttttct tgactgttct ccaataaggt
    32341 tctgctgatt caagcattga atcgtccggg taaccctctt actgactata ccccggcgtg
    32401 gtgtagaacg agagcggctg ctcgcgggtg tcaagccggc cgtattgcgc cacgaccgga
    32461 agcgagcact caagcttgat cgcatactgt gtgtcccgtt cgaggacgaa gcccgccaag
    32521 tcgcttgggt catccatccg aagacagcgg actcgattgg cttcgacgac caccggaata
    32581 tcgacgacgg gatccttgtc ggtgaaatag agcgtcatct tcacatcgac ggcctgttca
    32641 ttcatattca ggatgatgac cgactcgtgt cccttcagcg ggaaaggacc ggcgggcggc
    32701 aagtcaccat caggaaacac ccaaatcgtt ttaccaattg tgtttttcat gaaaacctcc
    32761 tactatcttt ttcatggaga gattacccat agtgtaccgt cagcacactc cgattgcaag
    32821 aaaacccttt cgttttaatt aaacaatatt tgaaacgttt tattgctaaa tccaattctc
    32881 tcatctatac tggactgcgt acacgatact caatgaaagg atcttgatgg tggctacgat
    32941 taaagatgtt gcaaagaaag ccggtgtgtc gattgcaacg gtgtccaatt acctgaacaa
    33001 cacaaacccc gttcgtaaag agactgcaac cagaatacag gctgcgatcg attcgcttgg
    33061 atacaaacaa aactacatgg cgcgaaacct gaaagcccag cggtataccg atgtcggggt
    33121 gattctaccc gatctcgaca atccgtacta tgttcaaata ttccaaggca ttgaaaaaac
    33181 attccaccaa agcaagtact atctgacctt ggcgttctcg tacgatgatc ccgagcggga
    33241 ggcggaaatc atcgacaacc ttctcaaaaa gcaggttgcc ggcctggtgc ttgttccaag
    33301 catggttgac aactggaaat tctattacca ccaccttgtt tcaagggaca agcccctcgt
    33361 catcctcgat cgacagatcg ataatttgga aacgaacttc atcgcctttg aaaatgagaa
    33421 agccatcgca gaccttgcca aatccctcat cgaccaaggc aagaggaatc tcgtcctcat
    33481 caccggtcct cggaagtacc gggaggaaca gctgtgcatc gatgcgtttc tggaagtctg
    33541 cagtcaaaac gatatcgaca tcaatcaggc gctccaggtc tactcggtcg gaacgagcaa
    33601 ggaagagaca ttcaggatca cgacaaatct ccttcgaaga tttttgcccg atgcgattct
    33661 gacgacgaac ggagcggggg cggttggcat catcgagagc ttgagcttcc tcgggtattc
    33721 gacgaatgat atccccgtgg ccactctcgg agaggagcgg tggaacaaat acacccactc
    33781 gatctcatcc ctgtcctcgc agcgcccggc gatccagatg ggcgagcagg ccgcccaatt
    33841 gctgcttgaa cagcttaaat ccccgtttgt cagcgagaac cgaaggatat tgatgaccga
    33901 caagattctc accaaggaac cccaccctgt acgatatgcc caatatcaac cgattcagat
    33961 cgatcgcaac ggacagacgc tgaaggtgct tctcctcgac acctaccaga cccacgcctt
    34021 aagcggattg ttcagcaact tcacctccgc caccgggatc gccgttgacg cgacgagcat
    34081 tccccacccc gagctgtttg aacgaattat ccaagagagc cgcaaggctc ccggagattc
    34141 cccgttcgac gtggtgatgt tcgacatccc ctggttgtac acccttgcca gctcgaaagt
    34201 ccttgccgat atatccccgc acattgagcg caaagagatc aatcccggca ttttcctgcc
    34261 ccgctcgttc gaatacttca gcagattcga aaaaggccac tatgggcttc ccttcatgta
    34321 tgcccctcag atcctgtttt atcgaaagga tcttttcgaa gaccctttca acaagacgga
    34381 attcgatcgc ctgttcaaat ccaagcttcg acctcctcag acatggaagg agttcaacgc
    34441 gatctgcgag ttcttcacga gccataccga ccatatctcc tacggaacgt cgattcccgc
    34501 ctcccacccg gagtatctgg cgcctgaaat ctatatgcgc ctcttctctt ccaacagtca
    34561 ggtgtttgat aagaacaacc aggtcacttt cagcaatcct cggacgttga aagcgtatat
    34621 cgacctcatc aaggtcatga agcacgtcaa gcccaactat cttcagacga gcgacatgga
    34681 tgccgtgaag gactttctac tcggcgagac ggcgatgctc atcacctatc cctccttcct
    34741 gtatgatgaa gagctcatct cggcgaaccc gggaatcctc aacaacatcg cccacagcaa
    34801 catacccggc aacgccccga tcctcggagg atggagtctg ggaatcttcg cccaatcgga
    34861 acagaaggac caggcgttcg atttcatcaa atgggcgtgc tccgacaaga tcagcgacta
    34921 ctttgcattg atgggcggct actcggccgt tgaaagcacc tatctcaacg atgagctcac
    34981 gaagctgtat ccctggtttc cgctctacca ttcgacctat aaaacgactc gtccgatcct
    35041 gccgccgcga accgcatcgg ggaagatcgt ttcacagaac aagatcgact cgatcatctg
    35101 ccatggagtc tatgaactgt tgaaggactc gagcgacatc ccctcggtca tcgagaagac
    35161 ccacaaggcg ttggaggagc tgattctttc ccatgaagaa acgacctgat ccatggagaa
    35221 agaagcgtct ttcgcattga gcgccgggtc aaatgttccg gattaagctg attcgatttt
    35281 cgattatatc tacttgtaat tatatcatta taatgatact ataatgatat acggaggatt
    35341 tacatatgcc ggctattcgc cccagttccg atctgagaaa caattataat gagatatccg
    35401 aattctgtca gcaatacacc gaacccctat tcatcacaaa aaacggaaaa ggagacttgg
    35461 tggtgatgag tattgaaacc tatgagttac tcacgggaaa aatggagttg tacaacctct
    35521 tggatgaggg attaaaggct cgggatgccg gccaagtgga accgatgaac cgtgtgattg
    35581 ctgaaatccg cgcatcattg aaaaagtgat gtacgaaata caatggacat cgttggctaa
    35641 agaagatctg attctggcca cctcctacat ttccggtatt ctcaaagccc cggccacagc
    35701 tggaaaactg cttgacacca tagaacatga agttgaggct ctatcagaaa atccgtatat
    35761 gttctccacc tcacgagacg agaatctcgc gcaccgtggg atacggcact tctcggtgaa
    35821 aaactatatg ttgttctata ccatcaagga ggagacgaaa accgtaactg tcttgagatt
    35881 cttgcatgcc agacgaaatt ggattcaact gttgggccac ccctcactgt aagccgatcg
    35941 actgcaacag tatcagggtt cgtaattgga cgtgtcattt tagtcttcat ttttgatctg
    36001 tggggcgttt tgtaataacc tgtctctaat aaggcacatc ctcgaccgtt ttaacgctgt
    36061 atgcatttac attaaagaga agttgattcc ctcaacgttc tttctatttg caatgaatag
    36121 acctgcttca gatcgatcat ctctacattg atcaatgaat gctcaacatc atgaaacccc
    36181 gacatggaaa tgatccaata gaacttccta tcggtcttga acatctttga tttctcgaca
    36241 agctctgcca gcaccttccg gtgagccttc tcatttcgcc acgtgcactc gatgaacagc
    36301 atcgcctccg cattgaagga taggatatcg atctcggatt cctgctttcg caggggatca
    36361 ttgccccacc aggttccata atcttccggc aaaaaaggaa cccggtcgtt tttcaagcgc
    36421 tgctcatagt attgaagaca gatcttttcg aagaccttgc ccatgtattc attgattcgc
    36481 ggttcgatcc gctgccgata gactttcttc ccaagcccca gattgatcag gctgagattg
    36541 ggctggacga acgcatgcca gaattgaaac atcgtatccg aaacagcata tacgggccgt
    36601 tttgttttcc gatccagaac gggagtcttt cgctcaatga tgcccaagtc catcagattg
    36661 ttcagataag ggttcaagga acccgactgg acgcccagct tgttcgccag atcggacacc
    36721 ctgctcgtac ccgtgctcag cagatacaga atatcgcact atgcaatagt acgatattgc
    36781 atagtatgat attgcgtacc catatcccat aaaacggatc gccaatgctg ttgaaatcag
    36841 atacactatg gatgaataca ctcaaaaacc ttattgacga gagacatatg atcctgcctg
    36901 aacacatccc aacgctcttc aaagaagaac tgaccacctc gattcttccc ttctggctca
    36961 agcatggcct cgatccggtc catggcggga tgctcaccgg tctcgggcgc gacggatcgc
    37021 ttcttgaaag cgacaagagc atctggtttc aaggaagggc cgcctggacg ttcgccaccg
    37081 cctaccgcgt tgcccggaag aacgaggagt atctgctggt agcgaagtcc tgtctggatt
    37141 ttctgaaaaa ccacggtcag gacgatgacg gtcgcttctt cttccgggtt tcccgggaag
    37201 ggaatcccgt catcaagcgc tcccgctacg tcttcagcga gctcttcgcc gccctcggag
    37261 gagcggccta tgcccggacg accggtgaag attcttcgtg ggcggaaacg atcttcgatc
    37321 aggcgctcgg cacggtggaa gcaggcatcc tccagcccaa attcctccgc ccgagcgaag
    37381 gcttcgccct tccgatgatc gtcatcgcgg tggcccagga gctgagggct gcgaatccga
    37441 aacgaaccaa gcattacaat gctctgattg atcagttgat cgagaagatt caacggttgt
    37501 ttgtcaatga ggagcatcgc tgcgttctgg agcagaccgg aatcgacgga acaccccagt
    37561 tcgaccattt tgaaggccgc cttctgaacc ccggacacgc gatcgaaggg gcctggttca
    37621 tcatgaaaga agcccgtctc cgaagagaca gggatgatta cttctccttg ggaaagaaga
    37681 tgctcgactg gatgtgggaa tggggctggg acagggaata cggaggaatc atctacttcc
    37741 gggatgtatt ggggcacccc tcccacgaat actggcacga catgaagttc tggtggcctc
    37801 agaccgaagc ggtgatcgca acgctgcttg cctatcatca aaccggtgat gaccactatc
    37861 tcacgctctt caaccaagcc tatgattggg cgcttagaca tttcaaggat gaacgatatg
    37921 gggagtggta cggctatctg caccgcgacg gctcgctctc ggtcgacctg aagggcaatc
    37981 tctacaaagg gcccttccat atcccccgga tgttcctgga gggaatcgaa ctcttctcct
    38041 agcggctgtg gcggagcttt aagacctcga tgacctcttc gaggccgacc tccttctggg
    38101 tgaggagcac gaggaagtgg tagatcagat cggcggcttc gcccagaaag agctgcttgt
    38161 tgtcatcttt cgcttcgatg atcagctcga ccgcttcctc acccaccttc tgggcgatct
    38221 tgttcagccc gcggctgaag agatggttcg tataggaacc ttccagaggg aactcccgtc
    38281 gttcccggat cacttcctga agataaaaga ggaactcggg agcggaaacc tcctcggggt
    38341 tgttgacctc cccgaagcac gtgtctttcc cggtgtggca caccggcccg gtgggaaccg
    38401 ctttgatcag cagggtgtcg ttgtcgcaat cctcgatgat atcccgcact tccaggtagt
    38461 ttccgcttgt ctcgcccttc gtccaaaggg tttcccggct gcgcgaccag aacgtgacca
    38521 atccccggtc gcgggtggtc ttcagcgctt cttcgttcat gaacccgagc atcagcaccc
    38581 tgcgggtaac cgcatcctgg acaaccgccg gaaccaaacc gcccattttt tcaaatgaca
    38641 cagccataga ttgcatctcc ttagaaccct gtgctggtag ttataccacg ttgagcaaga
    38701 aaaatcttca gctttgggat atctatcgtc ccaaaatgaa ataccgacgc cccgagagcc
    38761 cccgaaacac cggtttcttc aaaaacccgg gcgaagtgct ccatcgttcc cgcaccgccg
    38821 ctggcgatga ccggaatgga gacggcggcg gcgacggctt tcgtaagggc gatatcatac
    38881 ccctccttca ccccgtcatg atccatcgag gtcagaagga tctccccggc tcccagcctc
    38941 tccacctctt tggcccaatc gaccaccttc cgatcggtga cgacccgccc gccttggacg
    39001 gcgacccgtc cctctttcgc gtcgatcgcc acgacgaggc gttccgagcc cacagcattg
    39061 gcgatagcgg caatcaggga gggatccctg accgccatcg agttgatcga gacctttgca
    39121 gctcccgctt ccagaagggc ttttgcgtct tcgacggaag cgatcccccc gccgacggtc
    39181 aagggaacgg aaataccctc ggaaacgccc cttacaaggt cgatgaaggt cttccgcttc
    39241 tcgacggtgg cggtgatgtc cagcagaacc agctcatcga ttccgagctc gctgtaatgc
    39301 cgggcgagcc cgatcggatc cccggcgtcc gagagtccga cgaagttcac ccctttgacg
    39361 acccgcccat ccttcacatc gaggcaggcg atgattcgtt tcctgttcat ccgttacacc
    39421 tcgccagttc atcaagggtg atagtccctt catagagcgc ccggccgatg atcgccccgt
    39481 ccattcccag atcccgaagc ctctcaaggt cgcggatcga agagaccccg ccgcttgcga
    39541 tcagcttcag cgaaggcatc gcaccgagga gctttccata gagttcatac gatggacctg
    39601 aaagcatccc gtccttggcg atgtcggtac agatgaccgt atcgaggcct tcatgaaggt
    39661 aggactcgat gaacgattgg acatcaagtc cgctttcttt cagccacccg tgggtccgga
    39721 tgattccccc tttgctgtcg gcgccgagaa tcaggcggtc caagccgaac ctctgaatcc
    39781 actccttgac caaagcgggt tcggtcaccg cgattgagcc gaccgtcaca tagcgcgccc
    39841 cggcgtcgaa cgcctgcctc aggtcctctt ccttcttgat ccccccgccg aaatcgatga
    39901 tgagagcggt gttccgggca agggtttcaa gcgtttcaag gttcacgatc cccccgccct
    39961 tcgccccttc cagatcgacg acgtgcaggc gcctgattcc atggtcttca aaggctttgg
    40021 ccacctccaa ggggtttgag gagtagatcg tcttttggtc gtagtcgccc tgggcaaggc
    40081 ggacgacctt cccttcgatc aggtcgatcg ccggaatgag atccatcaca gcaccccctt
    40141 cgtcgacggc agctcatcac tgtacggatt gcggtggatc gcctggcgga ccgcccgggc
    40201 gaacgccttg aacagcccct ccacctggtg gtgggcgttt cccgtaccca ccttcatatg
    40261 cagattgcac cgggcgctgt cggaaaacga cttgaagaag tgctcgacca tctcggtggg
    40321 gaaggttccg atatactccc gggcgatcgg gacgtcatag gaaaacgccg gacggttgga
    40381 aaaatccaag gcgacttccg ctttcacctc atccatcgtc agcacctcga atccgtaccg
    40441 gttgatcccc cgcttctccc ccaatgcact gcgaaacgct tcacccaagg cgatgccgac
    40501 atcctccacc gtatggtgct cgtcgatcat cagatccccg tcggcatgga gggaaagatc
    40561 acacccactg tgcctcctga tctgatcgag catatggtcg aagaagggga tgtgggtatg
    40621 gatctccccg agtcctcctc catccagcga gaggctgagg gtgatcgagg tctccttggt
    40681 tttacgagtg atatgagaaa ctcgcggagc caggttcggc tcgtcaagaa ggaaggaggc
    40741 gatctccccc caccggtcgg aaaccaggat gcatgtatca tgcaagtcgc cgggaagctt
    40801 cggctcatcg ctcttggggg tcagccatac cgccttgcac ccgatgttct tcgccagctc
    40861 gacatcggtg agccggtccc cgatgaccac cgagtgctcg aggttccacg atccgtcaaa
    40921 gaaggaggcg agcatccccg ttttgggctt tcttccggga cactcgtctg cgggaagcga
    40981 gtaatcgata tgctcctcat cgaagaggat cccctccccc cgcagcgttt ctttgatccg
    41041 ggtgtggacc ggttcaaaat cctccaaggg gaaagaggga gttcccaccc cgtcctgatt
    41101 ggagaccagc acgagataaa actgattgga atccaccagc cgtttcaacc ctccgaagac
    41161 tcccggaata tagcgaatct tctcgaagga atcgacctga tcttcaacga cgatcacccc
    41221 gtcgcgatcc acgaacaaga tcttcttatg cataatactt cctcatatac gcttcaaacc
    41281 cctcaaggag gatatcgttc tcagcttggc tgccgaccgt gatgcggacg cagttctcca
    41341 gcccccgctc cttcgtccga ttacggataa tcgtaccgtt ctccgtcagg aaggtataga
    41401 gccgggtcgc gtcatccact ttgagcagca ggaagttcgc gtcgctcgga tacacgatcc
    41461 gcacgcaacc aaacgcagca agcgctccgg aaagacggga gcgctccctc ttgatctcat
    41521 caaggcttct tttgaccgcc tcggcgtttt ccagcgccct gagggcgagg gcctgagaag
    41581 gggttccgag gttataggga tatttgatct tgttgatcac ctcgaccagc tctcggcttc
    41641 cgacggcgat cccgacccgg gcgcttgcaa gtccccacgc tttggagagg gttcgaagaa
    41701 ccactaagtg gtcatacttg gaaagaagcg aaaccccgct ctccccttcg ctgaaatcgt
    41761 agtacgcctc gtcaaggacc accaggccgt cgaagatctt caagaccgct tcgatcgttt
    41821 caagggaata gctgttgccg gtcgggttgt tcggcgaaca gagaaagaga agcttcagcc
    41881 ggccttcgct tcgctcattc cgttcctttg aaaggaagcg ctcaagggcg ggaaggtcga
    41941 tctcgaacga tggaagcagg gggagttcct ccacctccac gtcgttgatg tcggcgaaga
    42001 ccctgtacgc cccgtaggtc ggagggagga tcaggatcga atccttcccc ggcgtgcaga
    42061 cggcgcggat gaggttgtcg atcaattcat cgctgccgct gcccagaacc gtgtgttcga
    42121 agggaagacc gaggacctcc tctatcttct ttcgaagaac cgtcgccttc ggatcggggt
    42181 aacggttcgc atccttcatc tcgacgaaat cccggtagtg ctcattggcg tccagataca
    42241 cgtccgcttt tccggtgaac tccgttcggg cgctcgagta gggtttcagg ttgacgatat
    42301 tttttctgag cagttcgttc atcgctcttc ctccaatcgt atttcaacgg ctctcctgtg
    42361 ggcttcaagc tcttcatggg cggccatcgt catgacggtc ggagccaacc gggcaaggcc
    42421 atccccggtc agctcctgga ccgtgatctt cttcacgaag gaatcggtcg agactccgct
    42481 catcgaacgg gcccatccgc cggtcggcag ggtgtggttc gtcccgctgg cgtaatcgcc
    42541 ggcggactcg gggctgtagg gtccgacgaa gatcgaaccg gcgctttcaa taagatccac
    42601 cagagaatca tccagtacac gatctccggt gttgatgatg aggtgctcgg cggcgtaggc
    42661 gttggcgatg aaggccgctt cttcaacctc ttggacgacc gctattctgc tcgcctccaa
    42721 cgattgcttc acatacccgc tccgcttcaa aaccgaacat tgcctctcaa gttgctcctc
    42781 cagctcttcg atccaccgat cgggatcgga accgatgagc accaccatcg cctgactgtc
    42841 gggaccatgc tccgcctgac tgagaaggtc ggaagcgacg aaggcaggat tggagcgggt
    42901 ggtggcgacc accatcacct cgctcggacc ggcgggcatg tcgatcgcgc atgcaccttg
    42961 agcgacttgg gttttcgctt ccgtcacaaa gcggttcccc ggcccgaaga tcttgtcgac
    43021 ccgatgtatc gtctccgttc caaaggcgag cgccccgatc gcctgagctc cgccgacctt
    43081 gtagatctca tccacgccgc aaagctcggc ggcatacaga atcgccggat ggatcttccc
    43141 ttccttgtcc ggcggagtgg cgaggacgat ctccctgcag ccggcgatcc gcgccgggag
    43201 ggccagcatc aggacggtgg aaaagagcgg agcggtgccg ccggggatgt acaaccccac
    43261 ccggcggatc gggacgacct ttcgcttcag ggacaccccc tccatcacct cgatctcttc
    43321 acccactgcc cgttgggcct catgaaacgc ccggatattg ctcttcgcca aggcgagcgc
    43381 ttccttgagg tccggatcaa ccgcctttct cgcttcctcg aactcttccg agctcacggc
    43441 aagcgaagag aggacgaccc cgtcgaagcg ctccgaatac cgatggagcg cggcatcgcc
    43501 ttcctcgttg accgcggcaa gaatcgttgc cacctgctct tccaccgagg agcgatcaag
    43561 gaggttccgg gtgcagaaga ggtcaaggtt cttccccttc gggttgtcaa accgctctaa
    43621 gtatggcatg gctactccgt catcttttca atcgcggtca ccagaatgcc ctgggcgccg
    43681 agggctttca gctgttcaaa cacttcccag aacgtgtctt cggcgacgac gatctgaact
    43741 gagacccatc ccgtctccat cagcttggtg acggtcgggc tcttcattcc cccgaccacc
    43801 gccgccaccg cttccagctg ggcttcgggc aggttgaaga tcacatactt gtatgatgag
    43861 gccttcatca ccgcatcgat cctgagcagc aggcgggaaa gaatctcctc cttcaccttg
    43921 gagcccatct ctttccgccc gatcatcacc gcgctggacc gatagatcgt ttgtacttct
    43981 ttcaggccgt tcatggcaag ggtcgctccg gtggagacca gatcgcagat cgcatcggca
    44041 acccccacca gcggggtgat ttccaccgcc cccgagacgg acacgaggtt cgccttcacg
    44101 ccctcctctg caaggatgct ctccagaatc ctcggatagc tggtggcgat ccgctttcct
    44161 tccagactcg aaaggtcttt gtagccgaac tcatggggaa cggcgatgct gaggcggcac
    44221 ttggcgaacc ccagatcccg aagcacggca aggtcgatcc cctgctcgtc gaattcattc
    44281 cggccgacga ttccgatgtc ggcgatcgaa tctgcgatgt aggaggggat gtcatcatcc
    44341 cggacataga gaaattccag agggaagttc gttgcccggt ccttcaggac ccgctcgtcc
    44401 gctccgaact tgatgccgca ctgcttgatg atctcaagcg attgctcgct gagtcgtcct
    44461 ttcttttgga tcgctatccg taacacttca tccatcgctt cctccaaacg aataaaaaaa
    44521 gacccccgcg acagcggagg cgtgtattta tgacaattcc acactcaccg gtcgggcagc
    44581 gtcgcatcaa gatggtgatg gtggatatgc ttcttcatac cgctacgata ccgtcggtct
    44641 ccactttctg tcaagacggg ccccgattgt ggagagtttg tgtacatgat ccaacacgta
    44701 ttgccaactg tataaacccc ctgttatatc catgcttccg cagtaactaa ccaagaccag
    44761 tagagcgtag ttttatttgt aagaaattac gcttcttttt tatctttttc tattgacttt
    44821 atatcgtagg gtat
//
LOCUS       DAOSLI010000010        42254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1074_length_42254_cov_28.420010, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000010 DAOSLI010000000
VERSION     DAOSLI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 42254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42254
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1074_length_42254_cov_28.420010"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..2736)
                     /gene="ppdK"
                     /locus_tag="PLF67_02745"
     CDS             complement(34..2736)
                     /gene="ppdK"
                     /locus_tag="PLF67_02745"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOE88967.1"
                     /translation="MTNKKKKYVYFFGSGKVEGTVKMREILGGKGANLADMTSIGLPV
                     PPGFTISTEACAYYSANDGAYPEGLREEILEHLATLEEMMGAKLGDETNPLLVSVRSG
                     AASSMPGMMDTILNLGLNPKSVQGLIAKTGNERFAWDSYRRFMQMFGDVVMGVPHALF
                     EGALQDVKDERGVKLDTDLDAADLQEVIKRYQKIYHDTVGTDFPYDPVEQLFLATNAV
                     FKSWNNERAIRYRQMNDIRGLLGTAVNIQSMVFGNMGETSGTGVAFTRDPSTGENHFY
                     GEYLMNAQGEDVVAGIRTPEPIETLERVNPEAYRQLVDIRHLLEQHYKDMQDLEFTIQ
                     EGTLYILQTRSGKRTVFSWLRSQVEMVEEGLITKEEAVSRVPAGEFGKLFAPVLDSKY
                     ISDHGVDVITKGLNASPGGASGKAYFTAAKAEEMAAKGEDVILVRNETSPEDIGGMAV
                     AKGIVTARGGMTSHAAVVARGMGCPCVSGAHEIVINEEKKFLTVGGVTIKEGEYIALD
                     GFTGEVYAVEVPVKASEIVQVLNGQMKEEESQLFGFYKKFMSYVQEIKRMAVYTNADT
                     PQDTRMAVLFGAEGIGLCRTEHMFFGEGRILPMRKMILAGNTIEREKALDQLLPMQRG
                     DFEGIYEALAGRPATIRLLDPPLHEFLPNDHTSRHELALQMGIPVEELSQKISSMHEF
                     NPMLGFRGCRLGIIYPEILRMQVRAIIEAAINVKRKGIDVLPEIMVPLVGTYKEFVYT
                     KKIILEVIEAIFAEQKMEIEYKIGTMIEVPRAALTADEIAPHAEFFSFGTNDLTQMAC
                     GFSRDDSASFLEPYVNDPDKGFYDYDPFETMDFDGVGKLVRMASDLGRTANSDLVLGI
                     CGEHGGDPKTIEFFNEVGLDYVSCSPFRVPIARLAAAQAVIKQK"
     gene            complement(2924..3727)
                     /locus_tag="PLF67_02750"
     CDS             complement(2924..3727)
                     /locus_tag="PLF67_02750"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HOE88968.1"
                     /translation="MDFLKDQFSVKGKVAWVTGGSYGIGLSIALAYAKAGAKIAFNDI
                     NQELVDKGLAAYKEAGFDVKGYVCDVTDEQAVQKTARLIEQDLGPVDILVNNAGIIRR
                     TPMHEMSVEDWRLVVDIDLTAPFIVAKAVLPSMMERRSGKIINVCSMMSELGRETVAA
                     YAAAKGGLKMLTRNIASEYGKYNIQCNGIGPGYIATPQTAPLREKQADGSRHPFDSFI
                     IAKTPAERWGTPEDLMGPALFLGSKASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(3733..4575)
                     /gene="kduI"
                     /locus_tag="PLF67_02755"
     CDS             complement(3733..4575)
                     /gene="kduI"
                     /locus_tag="PLF67_02755"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOE88969.1"
                     /translation="MDIRYSTGKEAFKHMTTDELRKEFLIQNIFKADDVTGVYSHVDR
                     IVTLGAMPVKGKLNLSKNIDPMKDFGVNYFLERRELGMINIGGDGVVEADGVVYDLVH
                     YDGLYLPKETKEVFFSSKDPSNPAKFYMSSTPAHQAFPAKHIVFAEAKHVPAGAAADS
                     NKRTIHQYIHPEVLDTCQLSMGLTELESGSVWNTMPAHSHERRMEVYFYFEVKKDQTV
                     FHLMGEPTETRHIIVHNEEAVINPSWSIHSGVGTSNYTFIWSMAGENRTYTDMDWIAT
                     EDLR"
     gene            4705..5481
                     /locus_tag="PLF67_02760"
     CDS             4705..5481
                     /locus_tag="PLF67_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOE88970.1"
                     /translation="MAKAPTVQSLDRTFDILEVLGSSQEGRTLAMITDDLKLPKSTVH
                     RLLSVLISREYVRKNDETGRYQLGPGFISLSANYLNNLELKTESAPHLAELSASTGNT
                     VFLGIRRGTKMVYIDSKEQINSLRKYEIIGQRKSLYSTGLGKALLLGLEEDEIRELLA
                     DEVFVPYGPNTHPNLDALLADIRISKERGWTVDDQEAEKAINCVAAPIYDYRGQVIAA
                     VSASWLLAQRPELTPALMAPSVVRCALNISYSMGYFRAKP"
     gene            complement(5533..6420)
                     /locus_tag="PLF67_02765"
     CDS             complement(5533..6420)
                     /locus_tag="PLF67_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOE88971.1"
                     /translation="MSTKRRMLRNDGKNTGYDESNILSIYLKEINRIPLLTEEEELDL
                     AIRAKAGDEFARNRMIESNLRFVVNVSKKYQNQGLPLSDLINEGNIGLMTALEKFDPD
                     KGYRFISYAVWWIRQSVMKAINEKGRAVRLPLNRTNELLQIKKAERTLMKELSTEDPT
                     AEEIGELTGFDPALVEDLLSISRELVSLDAPMLGDGSSLSSIGDFIEDDSVSPEDSLI
                     DVALQEDVGKILESTLNEREREIIELRFGLNGRFPMSLKEIGELYNLTKERIRQIEKK
                     ALDRLKAPEVSKYVEAYIA"
     gene            complement(6604..7050)
                     /locus_tag="PLF67_02770"
     CDS             complement(6604..7050)
                     /locus_tag="PLF67_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88972.1"
                     /translation="MDEERRLAIKRQELFPTADAPKQEIGCYFYRMAQLFAKMKDLER
                     SINCFIDAFLIRGMEERFKGEERWMSFFSRQFSLYLLGKNHLFCSLSEGDMIHDMLRM
                     EYEQVLEDLKNSELPVHPEHLDRWFSALEFDFPWNPPKVGQISPLV"
     gene            7192..7464
                     /locus_tag="PLF67_02775"
     CDS             7192..7464
                     /locus_tag="PLF67_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOE88973.1"
                     /translation="MTIKPLADRVLVKIEEVQEKTASGIYIPQTAQEKTQIGTVVAVG
                     EGTDKVKMLVKEGDRVMHDKYAGTSVKSNGDEYLILSMKDILAIIQ"
     gene            complement(7516..9612)
                     /locus_tag="PLF67_02780"
     CDS             complement(7516..9612)
                     /locus_tag="PLF67_02780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOE88974.1"
                     /translation="MTINDVEKIFDADGLLFQHFPLYEYREGQLLMAELIRRSYEEDA
                     IAVIEAGTGIGKSFAYLAVALFHALQNPDERSVIATSTINLQRQLVDKDIPALFAALD
                     MECSVALAVGRANYLCIQRYVQVKEASALLAQDPTGELYKVGAWVQETPTGLFAEYPH
                     PLSYELRSEIFCDPDLCNPQTCSYVHECFYFKARARWSKARIIVTNHHLLFSDARSRL
                     EGDLGYDEEHILPSYNRLIIDEAHNIEANATEFFTDIYSSKGLLHQASRIQRSSRYTP
                     NSLLEQLSPYSENPLGVDGIIEELVALNAEVQALDQYLLGVFSKNHYQPVLVTAEHQG
                     RLGTFKELAVEVAKKGKALSKRIFEFAQRCKVPEELESRLNELVVRSNRIDSMVEVLL
                     SFTDYGTWGEEVHWFNSERFGRDISVEVRITPLSVAPVLVEALFSKLQTVVSTSATLD
                     LHDEFEFWAKRVGLPYTDERPFFKGTFASPFEYTTHLLLITPEDAPLPAKELEEEYLA
                     YMSKTVLEATLASGGGALVLFTSYALLKELRSRLSEAYEEAGLLLLSQGELDRHTLLR
                     TFVEERDSVLFATSSFWEGVDAPGDTLRLVVITKLPFAVPSDPVFKARCDAIDAAGGS
                     GFFELSLKNATMKLKQGFGRLIRSSTDRGVVLILDSRLVQRRYGQFMLRSLPESFHQT
                     GPSDSIAAKIERFLFP"
     gene            complement(9599..10960)
                     /locus_tag="PLF67_02785"
     CDS             complement(9599..10960)
                     /locus_tag="PLF67_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE88975.1"
                     /translation="MKRNILVVDDEKNIRSGLSLALEMEGYETFTAEDGQAAWELINK
                     KGIDLVITDLRMPRMGGNELLNRITSSYPNIPVIVLTGHGTVENAVEAMRRGAIDFFT
                     KPVNLDRLTLVVKKALSAHDLAREHEELKAEVAQLKKRNKFEHIIGKSQKMVNLMETV
                     SQVAPTKASVLITGESGVGKELVADAIHELSTRAKGPFIKVHCAALTSTLLESELFGH
                     EKGSFTGAVAQKRGRFELADGGTIFLDEIGEIDMPTQVKLLRVLQERTFERVGGEASI
                     TVDVRIVCATNRDLLAEVQAGNFREDLYYRLNVINLVVPPLRERKEDIPLLMTSFLAR
                     YTKENNRPIEGFSAGAKRALLSYDWPGNIRELRNSIESAVVLARGTIIELEDLPPQVT
                     SAESSSTVSLSVGITLAEAEKHLIIATLELEGGNKTKAAQTLGIGRKTLHRKLQEYHI
                     DDK"
     gene            complement(10964..12145)
                     /locus_tag="PLF67_02790"
     CDS             complement(10964..12145)
                     /locus_tag="PLF67_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE88976.1"
                     /translation="MKNFVRRAIEKIDQLDSKQIVEILESQSREMEMLEATLESIEDG
                     FILADKHLRILYANSVTPTLVPMIRLKSYEGVALDEVLHDPDVLSFITANVEGREIEI
                     KEDEFTFQKGDQLQTVKVTVYPYKDGSSARETSFVIRLTDVTEYNRAEARLRRSESLA
                     SMTTMAAGVAHEIKNPLAAMGIHLQLLKKAYERNESLTFEDARRYIDVLDEEIARLNA
                     IVVDFLFAVRPIDVRIRLERITTLLDQVCDFVEPELNGEGIVLKRTYPATLPGLMIDN
                     NAIKQALLNIIKNAMNAIKDSGTIEVGAKIDSNNVVVSIKDTGVGIPEEALGKIFEPY
                     YTTKSGGTGLGLTVVYKIMKEHQGDIQVHSVVGTGTTVRLIFPIPSGEHLALEEVHSG
                     R"
     gene            complement(12142..13305)
                     /locus_tag="PLF67_02795"
     CDS             complement(12142..13305)
                     /locus_tag="PLF67_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88977.1"
                     /translation="MIAPFQPHLAALLGEQLASGTFSRVSLFGGDRYTLRMSTAIECA
                     RVLSCAHGGGEHCRCPSCTKFATLSYSDLVIVSQRDHRQRIEAALAAFLHDPNGPWRN
                     RLIQTIRIPLLGFHPILLEEAGSALQNTAARAEGMNNLLYELTAEGVLERNRAEEYVR
                     DLRKTLKDFYSGLPKSTTVSINQVRSIDSWVHQTNISGQLRFVILEGIEQTNVAARNA
                     LLKLLEEPPKGVYFFLISEHPSRIMPTILSRSRQYLFPPLSEENRRSFIASYGLTESN
                     YDSLRSFFLTQSGVNIEESRLLVDRLLASLIDGRALDSRTIASITAEIEKTNSYEHVL
                     SILGEAIAKSVLSRRYVAKAVALIDETYAAGLLYNQNQRFTFEALYYRLLEER"
     gene            complement(13302..13829)
                     /locus_tag="PLF67_02800"
     CDS             complement(13302..13829)
                     /locus_tag="PLF67_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOE88978.1"
                     /translation="MMQQVSISLFGFVFNIVDIIVFVLMIVGGISGAFVGFAQTFTRR
                     GGYLIGFMTALLLTRWMQPFLSDTLGLSVFFSSLLSFVLLFIIGYTLIRLFGSMLENA
                     FEAIGLGGVNAFLGFVWGVCEMVIVSAFILFFLESTSLSELTPYLEQSQFVLSFVHRF
                     MPDTVRIIKETASSL"
     gene            complement(13826..14884)
                     /locus_tag="PLF67_02805"
     CDS             complement(13826..14884)
                     /locus_tag="PLF67_02805"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOE88979.1"
                     /translation="MVVCFTGGGTLGHVYPALAVHQELSSSESYSGFWIGRNDPTERR
                     IVEEANLDFYSIPSGKLRRYPSLRTVTDGVKVLGAIGFAYRILKRRKPDLLFSKGGFV
                     SVPPVIAAHLLKIPVISHESDISPGLATRINARFSDLICVPFAEGFDRLGREKLRVTG
                     NPVRKDLLEAKQKPFALAQDALPLLLVLGGSGGSAAINALIEKTLDELLTICSVYHQV
                     GANAPLPSLRPNYTAVEFIGDELAALLHRADLVVSRSGAGVLEELKLFAKASILLPLG
                     RKTSRGEQEANARHLEAKGAAVVLYEPVDPDAFVCMVRSLIDDPLRREALARTMGSLG
                     DGSAARRIGEIILGWRNA"
     gene            15026..17770
                     /gene="secA"
                     /locus_tag="PLF67_02810"
     CDS             15026..17770
                     /gene="secA"
                     /locus_tag="PLF67_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOE88980.1"
                     /translation="MARSFLSTLFGTKQEKDLKHLYPIVQQVNDLESWAQGLADDQFP
                     DETQKLKDALRQGATLDDLVPKAFALAREAAFRVLGERHYDVQIMGAMVLHQGKILEM
                     KTGEGKTLTCVPAAYLNALEGKGVHIVTVNDYLAERDASWMGPVYAFLGLEVGVILSN
                     MDNEAKRRAYSKDITYGTNNEFGFDYLRDNMKWAQEEKIQPLHHYAIIDEIDSILIDE
                     ARTPLIISGQSEDDSAQVLGASKIASFLIECEKDPETGDYYEMDPLARFDRSAPAFDE
                     RGDYKLDEKQKRVTFTNQGLIHIEELLGKHKVIEGSIYDGQNFEYVHYVTQAVRALRL
                     YHRDVDYVVADGQVQIVDEFTGRVLHGRRYSEGLHQAIEAKERIKVLGQNRTLATITF
                     QNFFRMYDKISGMTGTADTEAPEFLKIYGLDVVVIPTNRPVIREDHPDLVFYNEQFKL
                     QAICQEIARVHKTGQPILVGTISIEKSELLSSLLKRMGIRHEVLNAKNHAREALIIEN
                     AGSKGAVTIATNMAGRGTDIKLGGSLEGRIRSQVGSEATREEYQAALEKIAPLWKKDY
                     EEVKELGGLYVLGTERHESRRIDNQLRGRSGRQGDPGTSRFYVSLDDPLMRLFASDNV
                     RIILGKIGMQSGEPIEHRMLSNAIEKAQKRVEDRNFEIRKHLLDYDDVLNEQRNFLYR
                     ERDAILASDDLVERVRELTRQLIEEEVSRVFAQTKDQKEQAKLLDKVTRTLMLASSPF
                     EGSFTKDAITAHLIESADKEIDEKLKITGNAPLNEFLRFNYLRQIDIRWQNHLVALED
                     LRDAVSLRSYAQKNPLVEYKVEGFEIFTQMLDAIGLFVVQTLQRVKITPQREQYRRTP
                     AQHKVVESHSQQLAFAHEGPAPAKENAVTTIRREGAKVGRNEPCPCGSGKKYKHCHGA
                     "
     gene            complement(17776..18750)
                     /locus_tag="PLF67_02815"
     CDS             complement(17776..18750)
                     /locus_tag="PLF67_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOE88981.1"
                     /translation="MARDYYDDMHDRNSSRRKRRRGPSKTLLGVIILGIAVCVAVIIL
                     WLQFSAALEVKPAQVIESQPPAAEEIVPQPVTPPKPSEPVHQAQRLDVRPPSSKSVQF
                     TSHIVQAGEDLHSIALQYGLKVQTLVSINEIRNVQAVTEGAALKIPDRDGRYYTVREG
                     DMLSTIVRTQNLPFGWVTLQELNGLANEQIKVGDKLFIPDTSSTASSQYTDIAPIQFI
                     RPTPGAIIARFDQESGVRIRTSNDEPIKAAADGEVADTGFEANGQSRYVILSHEGGYR
                     TGYHNLGSVEVIGGSSVVQGESLGLSLEPYLFFSIEQLGIPLDPEQFF"
     gene            18792..20438
                     /locus_tag="PLF67_02820"
     CDS             18792..20438
                     /locus_tag="PLF67_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE88982.1"
                     /translation="MSTSPNHRHINLFIALIGVALLVLAIRFGVLMLKRFQSDKRYED
                     PVVADRVVRGTIYDRNGRILAIQTPYWGVYFHLNKIKDLKLVSEVVAPFVQMSPGEII
                     ERTKAYTTYARISSRIDEDQVEPLLAALSKYNLLDSVNVEKRIGRTYPSLFHAAQILG
                     FTNAEGEGIEGLEYALEKQLNPYPRVGEEQITYGSDVYLTLDMDIQYALDVQLQEIAN
                     LYDPNIIMGIIMDAKTGDILALGSYPWYDPNAFSRSNPRQRTNNTISTLLEPGSVFKL
                     FSLASVLKAGEAKLDESFECTGSYSFQAGNSTVTINCSTAHGTVDPQMMIAKSCNGAI
                     AHWARQTDEEAFYQTLVDLGFTRSWDIGLPSRPRAQIADPSTWSARSEATIAFGQELL
                     ATSLHLATAATALGIDGDLLQPHLILRIIDASTGTIVKQREKEVVAHLFDQADTALIR
                     KGMHKATSDEGTGYKARVNDFEIGIKTGTAQLFNPEKNSYSDGSTLASSLALAPIEDP
                     AYIIYIGVEGGRGWGADLAAPGIGNLVQALISQGKLRKTR"
     gene            complement(20422..21336)
                     /locus_tag="PLF67_02825"
     CDS             complement(20422..21336)
                     /locus_tag="PLF67_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88983.1"
                     /translation="MEFLSHTASDYERALREVRSHHGGSVRIHARYDRLEKGKNLCEI
                     HYYLVGTKKQEEEDHASDALATLIAANDLPYSFIRPVEESLQNHNHSLLEREVEFRLL
                     HHLFDNLRFKEEVREREIYVAGPAGSGKTTMAAKIARFLRLRGQKKIALVEIGSSNDR
                     LLRMLAHDCGVGFERVNDLDEIARLENYDHLIIDLGNIHDVVPLLSTLQASSQLLVLV
                     FGGSSKVSDMLAVTRLLPQRIQAVLAFAKTDETHTLGNVLALANMANLPLFAFGEGAS
                     VAMGFSLANEQYIFSRLHGFTVELSEFF"
     gene            complement(21344..22702)
                     /gene="hslU"
                     /locus_tag="PLF67_02830"
     CDS             complement(21344..22702)
                     /gene="hslU"
                     /locus_tag="PLF67_02830"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOE88984.1"
                     /translation="MENSTVRKLDELKPKQIVAELDKYIIGQEAAKRTIAVAIRNRTR
                     RKKLPEEIRDEVSPKNIIMIGPTGVGKTEIARRIAKLSNAPFIKVEATKYTEVGYVGR
                     DVESIIRDLMSVALQQTKAELAEAQAEKVKARVEERLLDLFLPQMKNEPNVDIVAPEK
                     DERLRDRFRTMLREGKYDDRTIELNVQARKHVGIEVMGAPNMEEIQEAMASLGSIFGG
                     KGHVRKVTIKRAREILTEEEMEKAVDNDRAIDEAKERVEQMGIVFIDEIDKVAKSGSS
                     SGIDVSREGVQRDILPIVEGTSVSTKWGVIDTTHILFIASGAFHVSKPSDLIPELQGR
                     FPLRVELNDLTANDFYRILTEPQNALTMQYRELLKTEGVEITFEDAAIRRLSEIAFKV
                     NASHDNIGARRLFTIMERLLEELSFTADELSGQHISITEAYVDERLGEIVEDQDLSRF
                     IL"
     gene            complement(22706..23242)
                     /gene="hslV"
                     /locus_tag="PLF67_02835"
     CDS             complement(22706..23242)
                     /gene="hslV"
                     /locus_tag="PLF67_02835"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOE88985.1"
                     /translation="MNEFKGTTIVAIRKDGRVAIAGDGQVTYGDAVLKGNAHKVRRLY
                     DGKVITGFAGTTADAFTLFELFETKLKEYSGDLTRSAVELAKQWRTDRSLRQLQAMMV
                     VSDGKKIYLISGTGDVVDPERDAIGIGSGGNFALAAALAYLDTGADLSAEEIARKSVE
                     VAATLCIYTNDSIITEVL"
     gene            complement(23239..24132)
                     /locus_tag="PLF67_02840"
     CDS             complement(23239..24132)
                     /locus_tag="PLF67_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE88986.1"
                     /translation="MIEEQLIEQYLRYLTTVGNLSEHTIVAYRRDLMQLLAFWRQQGV
                     ALEEAALEDGWLYVKHLSKTRRLSEASVNRMISSARTFYDYLLKQERVYSNPFSLIVQ
                     RKARNRLPTILTRDEIQALLALKPSDAESMRDLLLFALLYDTGCRIGEAVSIKEDDLD
                     LKQRRILIVGKGNKQRFVFFGKKVLTRINEYLPVKRNQCASPYLFCSTSGNQLPFSTI
                     GSIFAAYRKKMGWQKEFTPHVLRHSFATHLIDRGADIRLVQELLGHASISTTQIYTHV
                     SQARLSKVVAEHHPHGRRNKR"
     gene            complement(24141..24905)
                     /locus_tag="PLF67_02845"
     CDS             complement(24141..24905)
                     /locus_tag="PLF67_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOE88987.1"
                     /translation="MRISRLLSIATLNLPTEEKRRLAKGGTSVTPSIPIQPMLRFFSR
                     PGNKIVFYGMTGYPPSLIDLENPPFMLSYSASLPEAGDRIIALAGSRQSDYEGLRAAY
                     ALGKALAETGFTLLVSNSNGFDRWARLGSQSADRRSFVLCDCGLGTKRITSNRSLRGE
                     HLISPFLPYEEVSPQSCLSRNVLTAALSTAVIIGQAPKRSGALHVAGCALDLGKDVFV
                     HQSGIGSKPNQEGSRFLHQSGAPIVGGDDPDQPVVV"
     gene            complement(24938..25825)
                     /locus_tag="PLF67_02850"
     CDS             complement(24938..25825)
                     /locus_tag="PLF67_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE88988.1"
                     /translation="MRRSGKRSAFLILEEYEHYLALQKRLSEATIEVYLFEAGLLLEA
                     FEDPELVTVQEIEAFIIASSEQRSLSPRSIAKTQSALRSFFTFLQLQKIRADNPLSLI
                     RTPKKEESLPQVATYDEIEHLFSVINTADYLGIRDKTLFEVIYSCGLRISEAASIDIE
                     DYQDCQLRVIGKRNKMRLIPVGEVAQASLAYYLQEARPHLLSRSKPSRALFIGRRGQR
                     LTRQAISKRFDEYATLAGLTMSVHTLRHSFATHLLQSGADLRSVQELLGHSDIQTTQI
                     YTHVDASTLQRFWEEFHPD"
     gene            complement(25809..27026)
                     /gene="ftsZ"
                     /locus_tag="PLF67_02855"
     CDS             complement(25809..27026)
                     /gene="ftsZ"
                     /locus_tag="PLF67_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOE88989.1"
                     /translation="MDFGMFDVEDIIGSEQTTATVIKVLGIGGAGGNAVNRMIASGLK
                     KVHFVAMNTDVQALQRSNAHIRLPIGKELTGGLGAGGIPEIGEKAAQESKEEIKREIE
                     DADMVFITAGMGGGTGTGAAPIVAELSKSMNILTVAVVTTPFAFEGKKKLTLAQAGIE
                     KLRKHVDTLIIIPNQYLLRVVENNTPIKQAFLMADEVLYMGVQGISELITEPGEINID
                     FADVRTVMKGKGDALMGIGFGEGANRAVDAARQAISNPLLESASIDGAKSVLVNLSGG
                     DSLTLQEYQDVVELVTENCADDALIIAGQAYNPELGEQIKVTVVATGFERKTEQVGVE
                     NTDMEMIKRRFSTPQTHQEREHAHEPEPPSIEVFRWEDLRKQLRQGASTSSDDYTIPT
                     VLRYGRGKDEEER"
     gene            complement(27039..28277)
                     /gene="ftsA"
                     /locus_tag="PLF67_02860"
     CDS             complement(27039..28277)
                     /gene="ftsA"
                     /locus_tag="PLF67_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOE88990.1"
                     /translation="MAGEKMLMGLDIGSSRTRAVIGSVNREGQLMVDSICEHPSEGVR
                     SGSIVNIEQTLKTIQTVINEAELQAGAEISDVIVGIGGGHIIGIPSTGVVGISSPEQE
                     IKRGDIFRSLEVARAFELPQDREILHTLVQDFQIDGQSGIKDPIDMLGHRLESRVLIV
                     TASSAISQNQRKCIQRSGLNVARVVLQSLADAEVVLSTEEKEMGTILINIGSGTTDMI
                     AYRNGAPIFTGGVNLGGDDVTNDIAYILNKTRAVAEQIKRESGHSYVPSVSNEEMIII
                     PQVGGMPAIKMPKRELSRIIEPRMAEIFSLLLDAFSKEHEGSGFGGGIVLVGGGALLS
                     GVTDLAGEVFRMPARLGFPEAIGGLDRSYINPHYTTVLGLLKSEARRLRENQPTGKVG
                     RKARTQGPASKLRGFFRTLF"
     gene            complement(28298..29044)
                     /locus_tag="PLF67_02865"
     CDS             complement(28298..29044)
                     /locus_tag="PLF67_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88991.1"
                     /translation="MIRRITIYGITALLLSVLLFLALSHLPYFTISQVQVKIDGPLKH
                     VPIEISNRLSTLVGQGIFRVSLLSTKKQIEQLPIVTSLVLTRTLPSTIKATIAFIDSP
                     VIVQSENKERSYLVDGRTLVPIESSDADLWTDHLLRIEVPLEYALLMERYGTDSVFAS
                     VMGLTRGLSGEASLITRIKYDNNSSNSFGKMVLDLPSLNARLWVREPVDPKHVQTALY
                     WIEQDRKENLSFLSSSIQRYDLYREALVRR"
     gene            complement(29041..30198)
                     /locus_tag="PLF67_02870"
     CDS             complement(29041..30198)
                     /locus_tag="PLF67_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOE88992.1"
                     /translation="MGSGYQNFDYQGSDPMPGSIEQPKSKSTVTFLIVTLLLTIFGLV
                     QLYSASYNEALLNGVPHYYYVVRQLQFVGLAAIGSVIIVFLPSKVLKAFAWPLALAAL
                     ALLLLTVFTPYGQEKFGSRRWLQIGPLPALQPSEFAKLALILLFASYQGKPKKILLPA
                     AVALLYAALVFLQRDYSTMLLIVVIAFSMLLYSGVRLANVVLLLLFLIPPLFAALFSQ
                     AYRIRRVASFFFPSLDPSGMNYQVNVSLKAIAKGRLFGAGLGKGTYKLGILPEVHSDF
                     IIASVAEELGFVGILFILFLFFLYALLGYNGAKRLIGRDPLLSALSYGVTTMVLSQAI
                     LNLAVITALAPPTGIPMPFFSQGGTNMFVVLIASALILNVLRHANRKEEAR"
     gene            complement(30203..31111)
                     /locus_tag="PLF67_02875"
     CDS             complement(30203..31111)
                     /locus_tag="PLF67_02875"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOE88993.1"
                     /translation="MGRRIVVVFLSFTITFVLSKVLLSLYRGQGTTAKAYLPDFHTNK
                     EGTPIIGGVAFIIGSSIATLFERDFFSPTILYPLIALLTFGGIGFFDDLLKERHRRGD
                     GFTSLGKLGLQLLVSILLVAVMPLRTTLTFRSMTLELGWWYYPFAVLFITSFVNAVNI
                     TDGLDGLASLSAIPSLLLLLSVQGAFSGALPGALIAFVWYNYPPAKTFMGDSGSHALG
                     AYIAVGALLANREVLILFAGGLFFLELFSSLVQIISIRGFGRKIFLIAPLHHALEAAG
                     FAEGKIVQTFVTLSWVFAILSFVMWG"
     gene            complement(31104..32456)
                     /gene="murF"
                     /locus_tag="PLF67_02880"
     CDS             complement(31104..32456)
                     /gene="murF"
                     /locus_tag="PLF67_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOE88994.1"
                     /translation="MKIALAGQYSKASVVRSSDRAITHYALDSRRCVSGSLFFTLRGK
                     NSSGIDYLKEAESNGAVAVLVHTEDIQRALNETSCAVLASDDVFQALMNLATAYRALF
                     PHVRTIGITGSTGKSTTKEAVGKIASALGPTTMTSGNLNSEYGLALSIMNLNSETKYG
                     VFELGIDHPGEMERMTKMASPSIGVLTNIGNAHLESMGSREVIAREKAKIFHPGIEAG
                     FISRHSLYGHLIQTLSPVELIRYSTRDVSAIDLGLDGWQISYQGVSFRIKAVGKHLLE
                     DVAGAIRVGAYLGARPVDIAHALEDFEPLVGRSTVRTSRVTIINDTYNASLDSTRSII
                     RYLSRLSWSGRKNAVLGPMKELGAESKQAHLEIAKTLIQSTFTKHFLYGREMEEAYQT
                     IKREKGKERVSFTQDFEELEGMVRDEVTAGDLVLLKGSRVVQMERLIPTIERGVALHG
                     "
     gene            complement(32453..32773)
                     /locus_tag="PLF67_02885"
     CDS             complement(32453..32773)
                     /locus_tag="PLF67_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE88995.1"
                     /translation="MEHEWFDQPQYEEKPSSSVVKHILVALMLIFTVVVLLLPLWQRG
                     IHRSLEIEYRSLVDNRILLEERTQLLRASISTKAIPEAVSAYSWQEEIFFTPITAVVA
                     RGDL"
     gene            complement(32763..33674)
                     /gene="rsmH"
                     /locus_tag="PLF67_02890"
     CDS             complement(32763..33674)
                     /gene="rsmH"
                     /locus_tag="PLF67_02890"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOE88996.1"
                     /translation="MNYVHTSIMPNELLTHLVPQSSDPIMVDATCGEGGHTHLFLSTY
                     PNLKVIGVDRDREIQEKARVRMEEFGPRFKPVNMWFDVFFKEAEDSSFDLILFDLGIS
                     MFHFNESQRGFSLRKGEPLDMRLDSEDAISAFDVVNRYDEKKLANVIYAYGEERYSRR
                     IARAIVQHRKTKTIATTDDLANIIYKSVPPAYRYQKIHPATRTFQAIRIEVNHELDRI
                     EPALTEALRTLKVGGRLAVITFHSLEDRPVKWLFKNLAQEDRPILNVLTKKPLVPGEE
                     EIARNPASRSSKLRVVEKIREEERHGA"
     gene            complement(33674..34129)
                     /gene="mraZ"
                     /locus_tag="PLF67_02895"
     CDS             complement(33674..34129)
                     /gene="mraZ"
                     /locus_tag="PLF67_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOE88997.1"
                     /translation="MVTGEFYITLDDKGRLLIPARLRHALSGDALYVTRGLETCLWLM
                     LPKDFEQLKDTIINGPHSMFDRRLRILQRRMIAPAQLCEFDKAGRINIPPSLRESVKL
                     LLKEESVLLGAGNYLELWNKEAYEAYLEESMDEFLDAAQSLSNLGREEE"
     gene            complement(34348..35940)
                     /locus_tag="PLF67_02900"
     CDS             complement(34348..35940)
                     /locus_tag="PLF67_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HOE88998.1"
                     /translation="MSNANVAFVLNAHLPFVRHPEYPRFLEEDWLFESISESYLPLLR
                     MLNRLKEEGLSFQLTISFSPPLIAMLTDNALQNRFVHYLQQHIELGQKEVQRCKDEQP
                     QFLEMAQHYLDGYIRNLHEYEDLYRMNILDGFKALEQSGHLELITTAATHAYLPLYEE
                     YPQAINAQIELGVQSFLTTFGHPPKGFWLPECGYYPGLEEHLKYHGIKWFQVASQSML
                     LSPDKATYGGYRPVRCPSGVAAFPRDYQATSLVWSNTTGYPTDNVYREFYRDIGYDLN
                     MDYIRPYIHEPEVRVFTGYKYWAITGDTDQKVPYVRAQAMERVKEHARNFHYVVNNRN
                     HRLRATMGGAQPLFFLGFDAELFGHWWYEGIDWLEEVIRTSKDRTKLVTPSSFLAEDA
                     TPLQTVRPAFSSWGQGGYSAAWLDGSNTEYYRHIHKAITRMEELATRFPDQTSLKRRF
                     LNQAAREVLLAMSSDWPFIIYCRTSVEYAKKRIEGHLKNVNLVYDDMCKNAVDTEWLV
                     KAEKRHTIFGDIDYNIFNSERI"
     gene            complement(35937..36677)
                     /locus_tag="PLF67_02905"
     CDS             complement(35937..36677)
                     /locus_tag="PLF67_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HOE88999.1"
                     /translation="MILINIDSLSTNELQHIAQMEGLKGWKTLDRDELISALEDWFEE
                     MDEGGIPVGNGHGTYGRYTNNLTNHRGKAAHFKDLPRTGELPPSYYDSTIHLLLRDPQ
                     WAYAYWSLPETIQGNKTSFDDEDHSGESYVIRVHQTELATGTTTSSDIAVEVKDQEWN
                     IHLPALGCSYSVELCRLHPDGNEEVVARSASVETYLPYWADRIEELAMDRDLFLLHFS
                     SLVTKTGEVAENVVLKDLALTVSKGVLS"
     gene            complement(36893..37894)
                     /locus_tag="PLF67_02910"
     CDS             complement(36893..37894)
                     /locus_tag="PLF67_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE89000.1"
                     /translation="MKKALVIVLSLVFAMGVVFAAGAAEKAAVPKIGIAVPSPDHGWT
                     GGIGWWADKAVDELQKQYPGKYEFRVLHADGYAKQISDVEDLMVWGMDYLVILPHESA
                     PLTPVVKEAHAAGVKCIVVDRGLTDTSFGYVNLAGNNSEMGKVSGIFMRDFMKANGLT
                     RYVAMGGMPVVIDGERMNAFFDEMTKEPTLVNLAGGRNYDFADWSTQKGLELMETYIQ
                     KYPTIHAVFCQDDDVMTGVLQAIKESGRKDIKLVFGGAGSKAAYEMIMNDDPLVKATA
                     TYHPSMVYDAIMYCLDVVEGRKSDAFHTAKAPLSVVLPSVLVDKSNVMDHYDAGSVF"
     gene            complement(37909..38883)
                     /locus_tag="PLF67_02915"
     CDS             complement(37909..38883)
                     /locus_tag="PLF67_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89001.1"
                     /translation="MAEHKIKEYIKNYRIKDHSLGMAFIALFVIATIVGWPNFLKVRN
                     LTNILRQVSYTGIIGLGMTFVIISGGIDLSVGSMAAFVGGVSIFFLNLFGPDSTLALV
                     LTFFFALVFGSLLGALNGVMVAKFRMAPFIVTLGTMSIFRSLIQYFSNAGTILSVNMG
                     FGKLGSSIFLGLPVPVWFFIIIGVALHIILNYTSFGRYLCATGANEQVARYSAIKVSL
                     VKFVPYVITGFTVGVTAIMWSSRLNCINPSDCAGYEMDAIAAAVIGGTLMSGGKGSII
                     GTMMGAVMLGIINNMLVMGGISAFLQQAVRGFVIIVAVLLQYNSDSNK"
     gene            complement(38876..40396)
                     /locus_tag="PLF67_02920"
     CDS             complement(38876..40396)
                     /locus_tag="PLF67_02920"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE89002.1"
                     /translation="MTKRPPILELEGIVKEFSTVRVLDQVNVSIYPGEIMGLVGENGA
                     GKSTLIKIICGIYEETSGTIRLDGKDIRIPDYITAKSLGIGLVPQEFNLINYLTVYEN
                     IFLGNERKKGVFLDRPKMREEAKAQLESLKMPIDVHRTVSDLSVAEKQMVEIAKAMVL
                     DARILIFDEPTTALTGVEVQHLFALMRKLKEQGVTMIFVSHKLGEVMTICDRVTILRD
                     GHLVSVDEIKDLDTTIIARKMVGRDFSQVFPDKIDSTGKPPLLEVEGLTCPDLLRDVS
                     FTLRKGEILGFAGLVGAGRTETMETIMGLRKMSSGTIRFDGKPVKISNPREAVSHGLA
                     YISEDRQGKGIVMDYDIPKNITLISLKEKYVKRGLIDKHSEEKSGKEYVKGFNIVAAS
                     HKAPLRFFSGGNQQKVYLARWIDTKPRVLILDEPTRGIDVNAKREIYEFIHHLADEGI
                     SCIVISSEMEEVIGMCDRVYVMREGRIAGHLEKEHITEENIVFNATGIQKGVKRDG"
     gene            complement(40396..41388)
                     /locus_tag="PLF67_02925"
     CDS             complement(40396..41388)
                     /locus_tag="PLF67_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE89003.1"
                     /translation="MARNVTIFTGQWADLPFEEVCKIISEMGYDGLEIACWGDHMDVK
                     KAATDPAYVKDRLATLKKYNLGCWALGNHLAGQCVGDAFGDPRLAGFAPKALAGDDEA
                     IRAWGIEQMKYTAQAAKNMGCYVVTGFMGSPIWKYFYSFPANTPELIEEGYQEILRLW
                     TPILDEFDKQGVKFALEVHPSEIAYDYYSTKKLFEVFGGRKTLGINFDPSHLQWQGVD
                     PALFFRDFADSIYHIHIKDSVVKLDGRSGILGSHLMFGDLRRGWNFVSPGHGDVNFDM
                     IIREANAAGYQGPLSVEWEDNGMDRLFGAREALELVRRIDFAPSTIDFDGAMEN"
     gene            complement(41403..>42254)
                     /locus_tag="PLF67_02930"
     CDS             complement(41403..>42254)
                     /locus_tag="PLF67_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE89004.1"
                     /translation="LEQGIHVVCDKPLTWKIEESEELQRLAEKKNLLFAVTYTYTGYP
                     AVKQMRKMVRDGLLGKIRFVNAEYPQDWLANPVDESSPIAPWRMDPEVSGISNCLGDI
                     GSHIENLVSTVTGLKIKRVCARLDSLVEGRTLDDNATVLVEYEGGAKGMYWSSQVAFG
                     YDNALRIRVFGEKGGLEWAQEDPDHFIFTPSDAPKQVFSRGRDPFAEEAQRYSRIPSG
                     HPEGVYEAFANIYLPFVTALAKHLRGEKLEASDLDFPSVDQGLDGVRFINRCVQSSKQ
                     GALWVEM"
BASE COUNT         9471 a        11892 c        11197 g         9694 t
ORIGIN      
        1 aaaagaatcc cgggccgaag cccgggaaga ttgctacttc tgcttgatca ccgcttgggc
       61 ggcagccaag cgggcgatgg gaacgcggaa gggggaacag gagacgtagt cgagtccgac
      121 ttcgttgaag aactcgatcg tcttcggatc gccgccgtgc tcgccgcaga tacccagcac
      181 gagatcggaa ttcgccgtgc gaccgaggtc gctggccatt cgaaccagct tgcccactcc
      241 gtcgaaatcc atcgtctcaa acgggtcgta gtcgtagaag cccttgtcgg gatcgttgac
      301 gtacggctcg aggaaggagg ccgaatcatc ccggctgaac ccgcatgcca tctgggtgag
      361 gtcgttggtg ccgaagctga agaactcggc gtgcggtgcg atctcatcgg cggtcaacgc
      421 ggccctgggg acctcgatca tcgtaccgat cttgtactcg atctccatct tctgctcggc
      481 gaagatcgct tcgatgacct caaggatgat cttcttggtg tagacgaact ccttgtaggt
      541 gcccaccagc ggaaccatga tctccggcag cacatcgatt cccttgcgct tcacgttgat
      601 cgcggcctcg atgatcgccc ggacctgcat gcgaagaatc tcgggataga tgattccaag
      661 ccggcagccg cggaaaccga gcatcgggtt gaactcgtgc atcgagctga tcttctggga
      721 aagctcctcc acgggaatgc ccatctgcag ggccagctca tggcggctgg tatggtcgtt
      781 gggaaggaat tcatggagcg gcggatcgag gagtcgaatc gtcgcaggtc ttccggcaag
      841 ggcttcgtag attccctcga agtcgccgcg ctgcatcgga agcagctgat ccagcgcctt
      901 ttcccgttcg atcgtgtttc ctgccaaaat catcttccgc atcggaagga tccgaccttc
      961 cccgaagaac atatgctcgg ttcggcagag cccgattccc tcggcgccga agagcaccgc
     1021 catccgggtg tcctgcgggg tgtccgcgtt ggtgtagacc gccatccgct tgatctcctg
     1081 gacgtaggac atgaacttct tatagaaccc gaagagctgg gattcctctt ccttcatctg
     1141 gccattcaga acctggacga tctcggaggc cttcacggga acctcgaccg catacacttc
     1201 gccggtgaat ccgtcaaggg cgatatactc cccttccttg atcgtaacac cgccgacggt
     1261 gaggaatttc ttctcttcgt tgatcacgat ttcatgagcg ccgctgacgc aggggcaacc
     1321 cattccccgg gccacgaccg ctgcgtggct ggtcatgccg cctcgagcgg tgacgattcc
     1381 cttggcgacc gccattccac cgatatcttc cgggctggtc tcgtttcgca ccaggatgac
     1441 gtcttcaccc ttggctgcca tctcttccgc tttcgccgcg gtgaagtacg ctttgccgct
     1501 tgcgccgccg ggggaggcgt tgaggccctt ggtgatcaca tcgacgccat ggtcggagat
     1561 atacttcgag tcgagaaccg gagcaaagag cttgccgaat tcgccggcgg gaacgcggct
     1621 gaccgcctcc tccttggtga taagcccctc ttcgaccatc tcgacctggc tgcggagcca
     1681 gctgaagacg gttcgctttc cgcttcgggt ctggaggata tagagcgttc cctcctgaat
     1741 cgtgaattca agatcctgca tatccttgta gtgctgctcc aggaggtggc ggatatcaac
     1801 cagctgcctg tatgcttcgg ggttcaccct ctcgagcgtt tcaatcggct cgggggttcg
     1861 gattccagca acgacgtcct cgccttgggc gttcatcagg tactcgccgt agaagtggtt
     1921 ctcgccggtg gaggggtcgc gggtgaaggc cacgccggtt ccgctcgtct cgcccatgtt
     1981 tccgaagacc atcgattgaa tgttgaccgc agtccccagg aggccgcgga tatcattcat
     2041 ctggcgatac ctgatcgccc gctcattgtt ccaggacttg aacaccgcat tggtagcgag
     2101 gaagagctgc tcgacggggt cgtaggggaa atccgttcca acggtgtcgt ggtagatctt
     2161 ctgatagcgc ttgatgactt cctgcagatc ggcggcgtcc agatcggtat ccagtttcac
     2221 gccccgctca tctttcacat cttgaagggc gccttcaaag agagcgtggg gaacccccat
     2281 gacgacatcg ccgaacatct gcatgaagcg gcgatagctg tcccaagcga agcgctcgtt
     2341 tccggtcttt gcgatgagac cctggacact cttcggattg agtccgaggt tcaaaatcgt
     2401 gtccatcatt cccggcattg aagaagcggc tccgcttcgg accgagacca acagcggatt
     2461 ggtttcatct ccgagctttg cgcccatcat ctcttcgaga gtggccaaat gctcaagaat
     2521 ctcttcccga agcccttcgg gatacgctcc gtcgttggcg gagtagtacg cgcacgcttc
     2581 ggtgctgatc gtgaagcccg gaggcaccgg aagaccgatg ctggtcatgt cggcgaggtt
     2641 tgcacccttc ccgccgagaa tctcccgcat cttgaccgtt ccttcaacct ttccggaacc
     2701 aaagaaatag acatactttt ttttcttgtt cgtcattcat gactcctcta aattatttca
     2761 gattgataac gaacaaatcc tatcggttcc tccgcttact gtcaatcaac agagagtcaa
     2821 tttcttgttc agaaactatt tgagcggagt aaaaagtaaa acattgtacc aaaaacggaa
     2881 acggggaagg tgcatccttc cccgtgattg attctgtatc accttacggt tgtttgccga
     2941 tgtaggcgag aattcctcca tcgacataga gcacatggcc gttcacgaag tcgctcgcct
     3001 tcgaaccgag gaagagagcc ggacccatca gatcctcggg agtaccccag cgctcggcgg
     3061 gtgtcttggc gatgatgaag gaatcgaagg gatggcgcga tccgtccgcc tgcttctccc
     3121 tcagcggcgc agtctgcggc gtggcgatgt acccgggccc gattccgttg cactggatat
     3181 tgtatttccc atattcactg gcgatgttgc gggtcagcat cttcaatcca cccttcgctg
     3241 ccgcataggc tgcgacggtt tctcttccca gctcgctcat cattgaacag acgttgatga
     3301 tctttccgct tcttcgctcc atcatcgagg gaagcaccgc tttggcgacg atgaaggggg
     3361 cggtcaaatc gatgtcgacg accagccgcc aatcctcgac actcatctcg tgcatcgggg
     3421 tgcgccgaat gatgccggcg ttgttcacga ggatgtccac aggtccgaga tcctgttcga
     3481 tcagcctcgc tgttttttgg accgcctgtt catccgtcac atcgcacaca tatcctttta
     3541 catcgaaccc cgcttctttg taggcggcaa gtcccttgtc caccagctcc tgattgatgt
     3601 cgttgaacgc gatcttcgct cccgccttgg catatgccaa tgcgatgctc aaaccgatgc
     3661 cgtaactgcc gccggtcacc caggcgacct tccccttgac ggagaattga tctttcaaaa
     3721 aatccatctt ctttacctca aatcttccgt ggcgatccag tccatatcgg tataggtccg
     3781 gttttcaccg gccatcgacc agatgaacgt gtagttgctc gtccccactc cgctgtgaat
     3841 tgaccacgag gggttgatga ccgcttcctc attgtgaacg atgatgtggc gggtttccgt
     3901 cggttcgccc atcaggtgga agacggtctg atccttcttg acctcgaaat agaagtacac
     3961 ctccattcga cgctcgtggg agtgggcggg catcgtgttc cacacactgc cgctttccag
     4021 ctccgtcaac cccatcgaaa gctgacaggt gtcgagaacc tcggggtgga tgtattggtg
     4081 gatcgtccgc ttgttggagt cggcggcggc tccggcggga acgtgcttcg cctcggcgaa
     4141 gacgatgtgc ttggcgggga acgcctggtg ggcgggcgtc gagctcatgt agaactttgc
     4201 gggattggag ggatccttgc tggaaaagaa gacttccttc gtttccttcg gaaggtagag
     4261 tccgtcgtag tgaaccaggt catagaccac tccgtccgct tcgacgaccc cgtctccacc
     4321 gatgttgatc atgccgagct cccggcgctc caggaagtag ttcactccga aatccttcat
     4381 cggatcgatg ttctttgaaa ggttgagttt cccttttacc ggcatcgccc cgagggtaac
     4441 gattcgatcg acgtgggagt atactcctgt gacgtcatcc gctttaaaga tgttctgaat
     4501 taagaattcc ttgcgtaatt catccgttgt catgtgcttg aacgcttctt ttcccgttga
     4561 ataacgaata tccattcttt ctctcctacg aattaatact agacgatcgc tgttccatat
     4621 tgtggaatac acttccatta tagtagtgtc ccaactcttg tcaaggcact tgaattggaa
     4681 tacattgatg ctacggaggc atttgtggcg aaagcaccta cagtacaatc tcttgatcga
     4741 acctttgaca ttcttgaagt gttgggcagt tcgcaagagg gtcgaaccct cgctatgatc
     4801 actgacgatc ttaagcttcc caaatcaacc gttcatcgcc tcctttcggt gctcatcagt
     4861 cgtgaatatg ttcgcaagaa tgatgaaacc ggacgttatc aattaggtcc cggctttata
     4921 tcattgagcg ccaactacct caacaacctc gaattgaaaa ccgaaagcgc tcctcacctc
     4981 gccgagcttt ccgccagcac gggcaatacc gtgtttttgg gaattcgccg gggcaccaag
     5041 atggtgtaca tcgacagcaa agaacagatc aacagccttc ggaaatatga gatcatcggc
     5101 caaagaaaga gcctttattc caccggcttg ggcaaagcat tgcttctggg gttggaagag
     5161 gatgaaatca gggagttgct cgccgatgag gtgtttgtcc cctatggacc gaacacccat
     5221 ccgaatctcg atgccctgct tgccgatatc cggataagca aggaacgggg ctggaccgtt
     5281 gatgaccagg aagcggaaaa agcgatcaac tgtgtcgccg ccccgattta cgattatcga
     5341 ggccaggtca tcgccgccgt atccgccagc tggctcctcg cccagcggcc cgaattgacc
     5401 ccggccctca tggctccgtc ggtggttcgc tgcgcgttga acatctccta ttcgatggga
     5461 tacttccggg cgaagccttg aagaagggaa aacggcaccc tctcgatgcc gtttcccgtc
     5521 aaaactatct gcttaagcga tatacgcttc aacatacttg ctgacctcgg gagccttgag
     5581 ccgatcgagg gcttttttct caatttggcg aatccgctcc ttcgtcagat tatacagctc
     5641 gccgatctct ttcagggaca tcggaaagcg tccgttcaat ccgaagcgca gctcaataat
     5701 ctcacgctcc cgttcgttca gcgtgctctc aagaattttc ccgacatcct cttgaagagc
     5761 cacatcaata agggaatcct ccggactgac cgagtcgtcc tcgatgaaat ctccgattga
     5821 actgagggaa gaaccgtctc cgagcatcgg cgcatcaagg ctgaccagct cccgactgat
     5881 cgaaagcaga tcttcaacca aggcgggatc gaaaccggtc aattcaccga tctcttccgc
     5941 cgtgggatct tcggtgctga gttccttcat caacgtccgc tccgcttttt ttatctgaag
     6001 cagttcattg gtccggttga gcggaagacg caccgccctg cccttttcat tgatcgcttt
     6061 catcacgctt tggcgaatcc accacaccgc atagctgatg aaccgatagc ccttgtcggg
     6121 atcgaacttc tccaaggcgg tcatcagacc gatgttgccc tcattgatca ggtcgctgag
     6181 cggcaggcct tggttctgat atttctttga aacgttgacg acaaaccgaa ggttggattc
     6241 gatcatccgg ttgcgggcga attcatcgcc ggctttcgct cgaatcgcca agtccaactc
     6301 ctcttcctcg gtgagaaggg ggattcggtt gatctctttg agatatattg aaaggatatt
     6361 tgactcatca tatccggtat tttttccgtc atttcgtaac attcgtcgct ttgtgctcat
     6421 ctatgcatct cccttgtgca atatatgatg caaccggcgt gccaattctt tgcaagttgt
     6481 ttataattta ttattatata agtagttata agtatttcac actggatatg tttcgtgaac
     6541 atgaacagat aatggcttat aagacaccat tttgggtcaa attgaccaag cagaaaaatt
     6601 tattcacaca agtgggctta tctgacccac tttaggcgga ttccaaggaa aatcgaattc
     6661 cagagcggaa aaccaccggt caagatgctc cggatgaacc ggcaattcac tgtttttgag
     6721 atcttcgaga acctgttcat actccatccg aagcatgtcg tgaatcatgt ccccttcgct
     6781 caaggagcag aacagatggt ttttcccgag cagatagagc gaaaattgcc tgctgaagaa
     6841 gctcatccac cgctcttcgc ctttgaagcg ctcttccatg ccgcggatca ggaatgcatc
     6901 gatgaaacag ttgatgctcc gctccaaatc tttcattttg gcgaagagct gcgccatccg
     6961 atagaagtag cacccaatct cctgcttggg ggcatccgcc gtgggaaaga gctcctgtcg
     7021 tttgatcgct aatcgccgtt cttcatccat gcaaggaata tactcataca tccggaaacc
     7081 ggcaaggtgt ccgtttttcg tacgttgctt gcctgttgat tgagatttta ctatagtatg
     7141 caaggcgagc aattcgctgt atgatgaaac caagttttga aggagatacg tatgactatc
     7201 aagccgttgg cagaccgggt actggtgaaa attgaagaag ttcaagagaa aaccgctagt
     7261 ggaatctata ttcctcaaac cgctcaggag aagacccaga ttggaaccgt cgtcgctgtt
     7321 ggcgaaggga ccgacaaggt aaaaatgctc gttaaggaag gagaccgggt aatgcacgac
     7381 aagtacgccg gcacctccgt gaagagcaat ggcgatgaat acctgatcct ttcgatgaag
     7441 gatattcttg caatcatcca ataactgcag cgatcactga acgaatgagg cgccttcggg
     7501 ttgcctcttt ttttctcatg gaaaaagaaa ccgttcaatc ttggcggcaa tcgaatcgga
     7561 tggacccgtc tggtggaagg attcggggag cgaacggagc atgaactgac catatcggcg
     7621 ctgaacaagt cgggaatcga gaatcagcac cacaccccgg tcggtggagg agcggatcag
     7681 ccggccgaag ccttgtttga gcttcatcgt cgcattcttc agggagagct cgaaaaatcc
     7741 gcttccgccg gcggcgtcga tcgcatcaca ccgagcttta aagaccgggt cggatggaac
     7801 ggcgaaggga agcttggtga tcacgaccag ccgaagggtg tcgccggggg catccacccc
     7861 ttcccagaag gaggaggtcg caaacagaac cgaatcccgc tcttcaacaa aggttctcag
     7921 gagcgtatgc cgatcaagct ccccttgact gagaagcaac agccccgctt cctcatacgc
     7981 ttcgctgagg cgcgatcgaa gctctttgag tagcgcatag ctggtgaaga gcaccagagc
     8041 tccaccgcct gaggcaagcg tcgcctccag aaccgttttg ctcatatagg caaggtactc
     8101 ctcctcaagc tcttttgccg gaagcggcgc atcctcgggg gtgatcagga gcagatgggt
     8161 cgtgtactcg aacggtgaag cgaacgttcc tttgaagaac gggcgctcat cggtataggg
     8221 caggcccacc cgcttcgccc agaactcgaa ctcatcatgc agatccaacg tcgccgacgt
     8281 ggatacgacc gtctgcagtt ttgaaaagag cgcttcgaca aggacggggg cgaccgagag
     8341 cggagtgatc cgcacctcga ccgagatatc ccggccaaac cgctcggagt tgaaccaatg
     8401 gacctcctcg ccccacgtgc cgtagtcggt aaaggaaaga agaacctcca ccattgaatc
     8461 gatccgattc gatcggacga cgagttcgtt caatcgactc tccagctctt cgggaacctt
     8521 gcagcgttga gcgaattcaa agatccgttt gctcagcgct ttcccttttt ttgccacctc
     8581 gaccgccagc tccttgaacg tcccaaggcg cccctgatgc tcagccgtca ccagaaccgg
     8641 ctgatagtgg ttctttgaaa acactcccag caggtattga tcgagcgctt ggacctcggc
     8701 gttcagagcg accagctctt cgatgattcc atcgacgccg agcggattct cggaataagg
     8761 agaaagctgt tcaaggaggg agtttggagt atatcgggag gaacgctgaa ttcgactcgc
     8821 ttgatgaagc agccccttcg aggagtagat atcggtaaag aactccgtgg cattcgcttc
     8881 gatattatgg gcttcgtcga tgatcagccg attgtacgag ggaaggatgt gttcttcgtc
     8941 atagccgaga tcgccctcca gacgggagcg ggcatcgctg aagagcaaat gatggttggt
     9001 aacgatgatc cgggcttttg accaccgggc tcgtgccttg aagtaaaagc attcatgaac
     9061 gtaggaacag gtctgcggat tgcacagatc gggatcgcaa aagatttcac ttcgcagctc
     9121 ataggagagg gggtgcgggt attcggcgaa caggccggta ggcgtttcct gcacccatgc
     9181 tcccactttg taaagctcgc ccgtcggatc ctgagcgagc agggcgctcg cctccttcac
     9241 ctgaacatat cgctgaatgc acagatagtt cgcccttccc accgccaacg ctacggaaca
     9301 ctccatgtcc aaagcggcaa acagagcggg gatatccttg tcgaccagct ggcgctggag
     9361 gttgatcgtg ctcgttgcga tcacgcttcg ttcatccggg ttctgcagcg catggaagag
     9421 ggcgacagcg aggtatgcga aggacttgcc gatcccggtt cccgcttcga tcaccgcaat
     9481 ggcgtcctct tcataggacc ggcggatcag ctccgccatc aacagttggc cttcacggta
     9541 ctcatagagg ggaaaatgct gaaacaacag cccgtcggca tcgaagatct tttctacatc
     9601 atttatcgtc aatgtgatat tcctgcagct ttcgatggag cgtctttctt ccgattccca
     9661 gcgtctgggc ggctttcgtc ttattgccgc cttcaagttc aagcgtggcg atgatcaggt
     9721 gtttctccgc ttcggcgagg gtgatcccca ccgagagcga aacggtcgaa gagctctccg
     9781 cggaagttac ctgtggggga aggtcttcaa gctcaatgat cgttccccgg gcgaggacga
     9841 ccgccgactc gatgctgttt ctcagctccc ggatgtttcc gggccaatcg taggagagaa
     9901 gcgcccgctt ggcgccggcg ctgaaccctt cgatcggccg gttgttctcc ttggtgtacc
     9961 gggcgagaaa gctcgtcatc aagagaggaa tatcctcttt tcgctcccgc agcggcggca
    10021 cgaccaggtt tatcacgttc aagcggtagt aaaggtcctc ccggaagttt cccgcttgaa
    10081 cttcggcaag cagatcgcgg ttcgtcgcac agacgattcg gacgtccacc gtaatcgaag
    10141 cctctccccc tacccgctcg aaggtccgct cctgcagaac ccgaaggagt ttcacttggg
    10201 tcggcatgtc gatctcaccg atttcatcaa ggaagatcgt cccgccgtcg gcaagctcga
    10261 agcgtccccg cttttgagcg accgctccgg taaaggagcc tttttcgtgc ccgaagagct
    10321 cgctctccaa aagcgtcgag gtcagagcgg cgcagtgaac cttgatgaaa gggcctttgg
    10381 cccgggttga gagctcgtgg attgcatcgg cgaccagctc cttgcccact ccgctctctc
    10441 ccgtgatgag gacgcttgcc ttcgtggggg cgacctgact gaccgtctcc atcaggttca
    10501 ccatcttctg gctcttgccg atgatgtgtt cgaacttgtt gcgcttcttt aattgagcga
    10561 cttccgcttt gagctcctcg tgttcccggg caagatcatg agcgctcagc gcctttttca
    10621 caacgagggt gagccggtcg aggttcaccg gcttggtaaa gaaatcgatc gcccctcttc
    10681 tcatcgcttc gacggcattc tcgaccgttc cgtgtccggt aagaacgatc accggaatgt
    10741 tcggatagga ggaggtgatg cgatttaaaa gctcgtttcc gcccattcgc ggcattcgaa
    10801 gatcggtgat caccaagtcg atcccttttt tgttgatcaa ctcccacgcc gcctggccgt
    10861 cctcggcggt aaatgtctca tacccctcca tctcgagagc gagggacagg ccggatcgaa
    10921 tattcttttc atcatcgacg actaggatgt tccgcttcat catttacctc cctgaatgga
    10981 cctcttcaag agccagatgc tcccccgaag gaatggggaa gatcagccga accgtggtcc
    11041 ctgttcccac gacggagtgg acctggatgt ctccttggtg ttctttcatg atcttataga
    11101 cgacggtcag ccccagcccg gttccaccgg acttggtcgt gtagtatggc tcaaaaatct
    11161 ttccaagcgc ttcttcggga atcccgactc ccgtgtcttt gatcgaaaca acgacgttgt
    11221 tcgaatcgat ctttgctccg acttcgatcg ttcccgagtc ctttatcgca ttcattgcat
    11281 tcttgataat gttgagcagt gcctgcttga ttgcattgtt gtcgatcatc agccccggca
    11341 gcgttgcggg gtaggtccgc ttcaagacaa ttccttcccc gttcagctcc ggttcgacga
    11401 agtcgcacac ctgatcaagc aacgtggtta tccgctccag ccggatccgg acatcgatcg
    11461 gccgcacggc gaagagaaaa tccaccacga tcgcattgag gcgggcgatc tcttcatcaa
    11521 gcacgtctat gtagcgcctg gcatcctcga acgtcaggct ctcgttgcgc tcatacgctt
    11581 ttttcagcag ttgcagatga atccccattg cggcgagggg gttctttatc tcatgggcca
    11641 cgccggcagc catcgtcgtc atcgaagcga ggctctcact gcgcctgagg cgtgcctccg
    11701 cccgattgta ctccgtcaca tcggtaagcc gaatgacgaa ggaggtctcc ctggcggagg
    11761 aaccgtcctt gtagggatag accgtcactt tgactgtttg aagctgatcc cctttctgga
    11821 aggtgaactc atcctctttg atttcaatct cccgcccctc gacattggct gtgatgaagg
    11881 agagcacatc cgggtcatgc agcacctcgt caagggcgac tccttcatag gatttgaggc
    11941 gaatcatcgg aaccagcgtt ggagttaccg agtttgcata gagaatcctc aggtgcttgt
    12001 cagcaagaat gaacccgtcc tcgatcgatt cgagggtcgc ctcgagcatc tccatctcgc
    12061 ggctttggct ttcaaggatt tcgacgatct gctttgaatc gagttgatcg atcttttcaa
    12121 tcgcccggcg gacaaagttc ttcatcgctc ctccaacaat cggtaataca acgcctcgaa
    12181 ggtgaaccgc tgattctggt tgtacagcag cccggcagca taggtttcgt cgatcagcgc
    12241 caccgccttt gcgacatatc ggcgggaaag gacgcttttt gcgatcgcct cccccaatat
    12301 actcaaaaca tgctcgtagc tgttcgtttt ctcaatctcg gcggtgatcg aagcgatcgt
    12361 tcgggagtcg agagcccgtc catcgatcag cgaagcaagc agccgatcga ccaggagcct
    12421 gctctcttcg atattcactc cgctttgcgt caggaagaaa gatcgaaggg aatcgtagtt
    12481 ggattccgtt aaaccgtagg aagcgatgaa gctcctcctg ttctcctcgc tcagtggagg
    12541 gaacaggtat tgacgactgc gggagaggat cgtcggcatg atccgggagg ggtgttcgct
    12601 gatcagaaag aagtatacgc ctttcggtgg ttcttccagg agcttcaaca gggcgtttcg
    12661 cgcagcgaca ttggtttgtt cgatcccctc caggatgaca aaacgcagtt ggccggagat
    12721 attcgtctga tggacccagc tgtcaatcga gcgaacctga ttgatcgaca ccgtcgtcga
    12781 ctttggcagg ccggaataga aatctttcag ggtttttcgg agatcacgga cgtactcctc
    12841 cgcccgattc cgttccagca ctccttccgc cgtcagctca tacaggagat tgttcatccc
    12901 ctccgcccga gccgcggtgt tctggagcgc ggagcccgcc tcctcaagga ggatcggatg
    12961 aaaccccaaa agcgggatgc ggatcgtttg aatcaaccga ttacgccacg gtccgttggg
    13021 gtcgtgcagg aatgcggcaa gcgccgcttc gatcctttgg cggtggtccc gttgactgac
    13081 gatgaccagg tcggagtatg acaaggtcgc gaacttggtg caggaggggc acctgcagtg
    13141 ttcccctcct ccatgggcac aggagagaac ccgggcgcac tcgattgccg tgctcatcct
    13201 cagggtgtag cgatcgccgc cgaagagtga aacccggctg aacgtgccgc ttgcaagctg
    13261 ctcgcccaac aaggctgcca ggtgcggttg aaacggagca atcataggct tgaggccgtt
    13321 tctttgatga ttcggacggt gtcgggcatg aagcggtgga cgaagctcag gacaaactgg
    13381 ctctgctcca gatagggggt caattcactc aacgaggtcg actcaaggaa aaagagaata
    13441 aacgcggaaa cgatgaccat ctcacagacc ccccagacaa accccaggaa tgcattgact
    13501 cccccaagcc cgatcgcctc aaaggcgttt tcaagcatcg agccgaagag gcggatcaac
    13561 gtatacccga taatgaagag gagaacgaac gacaggaggc ttgagaagaa gacactcagt
    13621 ccaagggtgt cggagaggaa cggttgcatc cagcgagtga ggagaagcgc ggtcatgaac
    13681 ccgatcaaat accctccccg ccgggtgaac gtctgggcaa agcccacgaa ggcgccgctg
    13741 attcctccga cgatcatcaa tacgaacacg atgatatcga cgatgttaaa cacaaagccg
    13801 aagagtgata tgcttacctg ttgcatcatg cattcctcca tcccagaatg atttcaccaa
    13861 tccttcttgc cgccgatccg tcccccaggc tccccattgt ccgtgccagc gcctcgcgcc
    13921 gcagaggatc atcgatcaac gatcgaacca tacaaacaaa ggcatcggga tcaaccggct
    13981 catagagtac cacggccgct cccttcgctt ccagatggcg ggcattcgct tcctgctcgc
    14041 ctctgcttgt ttttcttccg agcggaagca ggatgctcgc ctttgcaaac agcttcagct
    14101 cctccaacac ccccgccccg cttcggctga ccacgagatc cgcccgatga agcaacgcgg
    14161 caagctcgtc cccgatgaac tcgacggcgg tataattggg ccggagcgaa ggcagagggg
    14221 catttgcccc cacctgatgg tagaccgagc agatcgtaag tagctcatcg agggtcttct
    14281 caatgagggc gttgatcgcg gcgctgccgc ccgatccgcc gagaaccaac agaagcggaa
    14341 gagcgtcttg agccagagca aagggttttt gcttcgcttc aagaagatct ttccgaacgg
    14401 gattgcccgt caccctcagc ttctcccgtc ccagccggtc gaagccttcg gcgaaaggaa
    14461 cacagatcag atccgagaat cttgcgttga tccgcgtcgc cagtcccggg ctgatgtcac
    14521 tttcatggct gatcaccgga atcttcaaga gatgcgccgc gatcaccggg gggacggaga
    14581 caaacccgcc tttgctgaac aggagatcgg gtttccttct cttcagaatt cggtatgcga
    14641 atccgatcgc ccccagcacc ttgaccccgt cggtaacggt ccgaagggag ggatatcttc
    14701 gcagcttgcc cgagggaatc gaatagaagt cgaggttcgc ttcttccacg atccttcgtt
    14761 ccgtcggatc gtttcgaccg atccaaaagc ccgagtagga ctcggagctt gacaactcct
    14821 ggtgaacggc cagcgcggga tagacatgcc caagggtccc tccgccggta aagcacacaa
    14881 ccatacctcg agcatatcat cttccaatga gctcgtacag cggatttgta tcaatttgac
    14941 cctatgggta tatttgcccc atctcatcgt tttctctatt cggaattatc ccaaatgagt
    15001 atactcatac caaggcaggt atcatatggc tcgttcattt ttatccaccc tctttggaac
    15061 caagcaagag aaagacttga agcacctcta tccgatcgtt cagcaggtga atgacctcga
    15121 atcttgggcg caaggtcttg ccgacgacca attccccgat gaaacgcaga agctgaagga
    15181 tgctctcagg cagggagcga cgttggatga tcttgttccc aaggcattcg ctctcgcccg
    15241 ggaagccgca ttccgggtgc tcggcgaacg ccattacgat gtgcagatca tgggcgcgat
    15301 ggtccttcat caaggcaaga tattggagat gaagaccggt gaaggaaaaa ccctcacctg
    15361 tgtgcctgcc gcatatctca acgcgcttga aggcaaaggc gttcatatcg tcacggtcaa
    15421 cgactacctt gccgaacggg acgcttcgtg gatgggtccg gtgtatgcgt ttctcggtct
    15481 tgaggttggc gtgattcttt ccaatatgga caacgaggcc aaacggcgcg cctacagcaa
    15541 ggatatcacc tatggaacca acaacgagtt cggcttcgat tacctgcgcg acaacatgaa
    15601 gtgggcgcag gaagagaaga ttcagccgct tcatcactat gcgatcatcg acgagatcga
    15661 ctccattctg atcgatgaag cgcgaacccc cctcatcatc agcgggcaaa gcgaggatga
    15721 ttccgcccag gtgctcggtg caagcaagat cgcttcgttt ctgatcgagt gtgaaaaaga
    15781 tcccgagacc ggcgactact acgagatgga tccgctggcc cggttcgacc gctcggcccc
    15841 ggcgttcgat gaacgcggcg attacaagct cgacgaaaaa cagaagcggg tcacgttcac
    15901 caatcagggg ctcatccaca tcgaagagct tcttgggaaa cacaaggtga tcgaaggctc
    15961 gatctacgat gggcagaact tcgaatatgt ccactatgta acccaggcgg tccgtgcgct
    16021 tcggctctat cacagggacg tcgactacgt cgtcgccgac ggtcaggttc aaatcgtcga
    16081 cgagttcacc ggccgggtgc tgcacggccg gcgctacagc gagggtcttc accaggcgat
    16141 cgaagcgaag gaacggatca aagtcctcgg tcagaacaga acgctggcga cgatcacctt
    16201 ccaaaacttc ttcagaatgt acgacaagat cagcgggatg acgggaaccg ccgatacgga
    16261 ggctcccgag ttcctcaaaa tctacggcct tgatgtcgtg gtcattccaa ccaaccgacc
    16321 ggtgatccgg gaagatcatc cggacctcgt cttctacaac gagcagttca agcttcaagc
    16381 gatctgccag gagatcgccc gggtgcataa aaccggccag ccgattctcg tgggaacgat
    16441 ctcgatcgaa aagagcgaat tgctctcctc gctcctgaag cggatgggga tcaggcatga
    16501 agtgctcaac gccaagaacc acgcccggga ggcgctcatc attgaaaatg ccggctccaa
    16561 aggcgccgtc acgatcgcta ccaatatggc gggtcgaggc accgacatca aactcggcgg
    16621 aagcctggaa ggtcgaatcc gttcccaggt gggatccgag gccacaagag aagagtacca
    16681 agctgcgttg gagaagatcg ctccgctctg gaaaaaagac tatgaagagg tcaaagagct
    16741 cggaggcttg tacgttctcg gaacggaacg acatgaatca aggcgaatcg acaaccagct
    16801 gcgcggtcga agcggccggc agggagatcc gggaaccagc cgattctatg tcagcctcga
    16861 cgatcctttg atgcgcctgt tcgcttcgga caatgtccga atcatcctgg ggaagatcgg
    16921 gatgcaaagc ggtgagccga tcgagcaccg aatgctctcg aatgcgatcg aaaaggccca
    16981 aaaacgcgtc gaggatcgaa acttcgagat tcgaaagcac ctccttgatt atgacgacgt
    17041 tctcaacgag cagcggaact tcctctaccg cgaacgggat gcaatccttg catccgatga
    17101 ccttgtcgaa cgggtgaggg aactgacccg ccaattgatc gaggaggagg tttcccgggt
    17161 cttcgctcag acgaaggatc agaaggaaca ggcgaagctt ctggacaagg taacccgaac
    17221 cctgatgctc gcctccagcc ccttcgaagg ctctttcaca aaggacgcca tcacggccca
    17281 tctgatcgaa tccgccgata aagagatcga tgagaagctc aagatcacgg gcaacgcccc
    17341 cttgaacgag ttcctccgct tcaactacct gcgtcaaatc gatatccgat ggcaaaacca
    17401 tttagtggcg cttgaggatc tgcgggatgc ggtgagtctc aggtcgtacg cccagaaaaa
    17461 ccccttggtt gaatacaagg tggaggggtt cgagattttc acccaaatgc tcgatgcgat
    17521 cgggttgttc gtcgtccaga ccctccagcg ggtgaagatt actccccagc gggaacaata
    17581 ccggcgcact cccgctcaac acaaggtggt ggaatcccat tcccagcagc tcgccttcgc
    17641 ccacgaagga cccgctccgg caaaggagaa tgcggtaacc acgatccgac gcgaaggagc
    17701 gaaggtaggg agaaacgagc cctgtccctg cggaagcgga aagaagtaca aacactgtca
    17761 cggagcgtaa gaccgctaga aaaactgttc gggatccaag ggaatgccga gctgttcgat
    17821 cgagaagaag aggtagggtt cgaggctcag gccgagggat tctccctgaa cgaccgagct
    17881 gccaccgatc acttcaacac tgccgaggtt gtggtagccg gtgcgatagc cgccttcatg
    17941 actgagaatc acgtaccggc tttgcccgtt ggcttcaaac cccgtatcgg cgacttcgcc
    18001 gtcggcggcg gcttttatcg gctcgtcatt ggaggttcgg attcgaactc cgctttcctg
    18061 atcaaaccgt gcgatgatcg cccccggtgt cggtcggatg aattgaatcg gagcgatgtc
    18121 ggtgtattgc gaagaagcgg tggaagaggt gtcgggaata aaaagcttgt cgccgacctt
    18181 gatctgttca tttgccagac cgttcagctc ttggagcgtc acccagccga atggaagatt
    18241 ctgcgtccga acgatcgtgg aaagcatatc cccttctcga accgtataat aacggccatc
    18301 ccgatcggga atcttcagcg cggcgccttc ggtaaccgct tggacgttcc tgatctcgtt
    18361 gatcgatacc aacgtctgca ctttcaaacc atactggaga gcgattgaat gcaagtcttc
    18421 gcccgcttgt acgatgtggg aggtgaattg aacactcttg cttgaaggag gccgaacatc
    18481 gagtcgttgc gcttgatgta ccggttcgct cggtttagga ggagtaacgg gctgcgggac
    18541 gatctcttcg gcggcagggg gctgactctc gatgacttga gcgggtttta cctccagagc
    18601 agcactgaac tgaagccaca ggatgatcac tgcgacacag acggcgatac cgagaatgat
    18661 cacgcccagg agggttttgc tcggtccacg ccttcgtttt ctccttgagg aattccggtc
    18721 gtgcatatca tcatagtagt ctcgcgccat atctcgacaa tgcctctccc ccatagtagt
    18781 ataaatcgac catgtctacc agtccaaatc atcgccatat caacctcttc atcgccctca
    18841 taggggtggc gttgcttgtt ttggcgatca gattcggggt tttgatgctc aaacgcttcc
    18901 aaagcgataa acgatacgag gatcccgtcg tcgccgaccg ggtcgttcga gggacgatct
    18961 acgatcgaaa cgggcgtatc ctggcgatcc agactcccta ttggggagtc tacttccatc
    19021 tcaacaagat caaggatttg aagctggtca gcgaagtcgt cgcccccttc gtccaaatga
    19081 gccccggtga aatcatcgag cggacaaaag cctataccac gtatgcccgg atcagcagtc
    19141 gaattgatga agatcaggtg gagcctctat tggcggcact ttcaaaatac aatctcctcg
    19201 attccgtcaa cgtcgagaaa cggattggtc gaacctaccc ttctctcttt cacgccgctc
    19261 agattctcgg cttcaccaat gctgaaggtg aagggattga aggacttgag tacgctctgg
    19321 aaaaacagct caatccctac ccgagagtcg gggaggaaca gatcacctat ggaagcgatg
    19381 tgtatctcac cttggatatg gatatccaat atgccttgga cgtccagctt caggagatcg
    19441 cgaatctcta tgatcccaat atcatcatgg ggatcatcat ggatgcaaaa accggcgaca
    19501 tcctcgccct tggaagctac ccgtggtacg atcccaatgc attcagtcgc tcaaatcccc
    19561 gccaacggac gaacaacacg atcagcaccc ttcttgaacc ggggtcggtc ttcaagctct
    19621 tcagccttgc ttcggttctc aaagccggcg aagccaagct tgacgagagc ttcgagtgca
    19681 ccggcagcta ttccttccaa gccggcaact cgaccgtcac aatcaactgc tccaccgccc
    19741 atggaaccgt tgatccgcag atgatgatcg ccaaatcctg caacggagcg atcgcccact
    19801 gggcccgcca gaccgatgag gaggcttttt atcaaacgct tgtcgatctg gggttcaccc
    19861 gaagctggga tatcggcctc ccctcccgac cgcgcgctca gatcgccgat ccttcgacgt
    19921 ggtcggcccg cagtgaagcg acgatcgcct tcggccagga gcttttagca acctccctcc
    19981 acctcgccac cgccgccacg gcccttggga ttgacggaga tcttcttcag cctcacctga
    20041 ttctccgtat aatcgatgca agcaccggaa ccatcgtgaa acaacgggaa aaggaggtcg
    20101 tcgcccatct ttttgaccag gcggacactg cgctcatccg aaaagggatg cacaaagcga
    20161 cgagcgatga gggaaccgga tacaaagcgc gggtgaacga tttcgaaatc gggatcaaaa
    20221 ccggcaccgc tcagctcttc aatcccgaga agaactccta cagtgacgga tcgacccttg
    20281 cttccagctt ggcgctcgcc ccgatcgaag atcccgccta catcatctac atcggtgtcg
    20341 aagggggccg cgggtggggc gcagatcttg cggcgcccgg gatcggcaac ctcgttcaag
    20401 cgctcatcag ccaaggcaag ctcagaaaaa ctcgctgagc tcgacggtga atccgtgaag
    20461 ccgactgaag atatactgct cattcgccaa gctgaagccc atcgccacgc tcgccccttc
    20521 gccgaaggca aagaggggaa gattcgccat gttggcgagg gcaagcacgt tgcccagggt
    20581 atgggtctca tcggttttgg cgaaggcgag aacggcctga atccgctgcg gaaggagtct
    20641 ggtaactgca agcatgtcgc tgaccttgga ggacccgcca aacaccaaga ccagcagctg
    20701 tgaggaggct tggagcgttg aaagaagcgg cacgacgtca tggatgttcc ccagatcgat
    20761 gatcagatga tcgtagtttt ccaagcgggc gatttcatca agatcgttga cccgttcaaa
    20821 cccgaccccg caatcatgag caagcatcct cagcaggcga tcgtttgacg atccgatctc
    20881 gaccagagcg atcttcttct gaccgcggag acgaaggaaa cgggcgatct tcgccgccat
    20941 cgtggtcttt ccgcttcctg caggtccggc gacatagatt tcccgctctc gaacctcctc
    21001 tttgaagcgg aggttgtcga agaggtggtg aagcagccga aactcgacct cgcgttcgag
    21061 cagcgaatga ttatggttct gaaggctctc ctccaccggt cggatgaacg agtatggaag
    21121 atcgttcgcg gcgatcaggg tcgccaaggc atcggaggca tggtcttcct cttcctgctt
    21181 cttcgttcct acgaggtaat agtggatttc acacaggttc tttccctttt cgagacgatc
    21241 atatcgggca tgaatccgga cgctgccgcc atgatgtgaa cgaacctccc gaagggctcg
    21301 ttcatagtcg ctggcggtgt ggctcagaaa ttccatatca ctcctacaga atgaaccggc
    21361 taaggtcctg atcttcgacg atctcgccaa ggcgctcgtc gacatacgct tcggttatcg
    21421 aaatatgctg tccggaaagc tcgtcggcag tgaaggaaag ctcctccaaa agccgctcca
    21481 tgatcgtgaa caaccgtcga gctccaatgt tatcatgtga agcgttcact ttaaaagcaa
    21541 tttcactgag ccggcggatt gccgcatctt caaaggtgat ttcaacgccc tccgtcttca
    21601 aaagctctcg atactgcatc gtcaaggcgt tctgcggttc ggtgaggatt cgatagaaat
    21661 cattcgccgt cagatcattc agctcaaccc gaaggggaaa tcgcccctga agttcgggaa
    21721 tcagatcact gggcttggag acgtggaacg ctccgctggc gatgaaaagg atgtgggtgg
    21781 tgtcgatgac tccccacttt gtcgatacgc tcgttccttc aacaatcgga aggatgtccc
    21841 gctgaacccc ctcccgggat acgtcgatac cgctggaaga tccgcttttg gcgaccttgt
    21901 cgatctcatc gatgaaaacg atccccatct gttcgacgcg ctccttcgct tcatcgatcg
    21961 cccggtcgtt gtcgaccgct ttctccatct cctcttcggt gaggatttcc cgggcgcgct
    22021 tgatcgtaac ttttctcacg tgccctttgc cgccgaagat cgaaccgagg ctcgccatcg
    22081 cttcctgaat ctcctccatg ttgggggcgc ccatcacctc gatccccaca tgctttctgg
    22141 cctgcacgtt caattcgatc gttcgatcat catacttgcc ctcgcggagc atcgtccgaa
    22201 accgatcgcg caagcgctca tccttttcag gagcgacaat atccacattg ggttcatttt
    22261 tcatttgagg aagaaacaaa tccaacagcc gttcttccac ccgggctttc actttttccg
    22321 cctgagcctc ggcaagctcc gccttcgtct gctggagggc tacgctcatc agatcgcgga
    22381 taatcgactc cacgtcccgt ccgacatacc ctacttcggt gtatttcgtc gcttccacct
    22441 tgatgaacgg agcgttggac agcttggcga ttcggcgggc gatctccgtc ttgccgaccc
    22501 ccgtgggtcc gatcatgatg atgttcttcg gtgaaacttc atcgcgaatc tcttcgggaa
    22561 gcttctttcg gcgggtacga ttgcgaatcg ccactgcgat cgtccgtttg gcggcctcct
    22621 ggcctatgat atacttatca agctcggcga cgatctgctt cggcttgagc tcgtccagct
    22681 ttcgaacggt ggaattttcc ataccttaca atacctcggt gatgattgaa tcgttggtat
    22741 aaatacacaa cgtggcggcg acttcgacac tcttgcgggc gatctcctcg gcgctgagat
    22801 ccgcaccggt gtccaagtaa gccaatgcgg cggcaagggc gaagtttccg ccgctgccga
    22861 tgccgattgc atcccgctca gggtcgacca catcgccggt accgctgatc agatagatct
    22921 tcttgccgtc actgacgacc atcatcgcct gcagctgcct cagggagcgg tcggtccgcc
    22981 actgcttcgc cagttcgacg gcgcttcggg taagatcgcc gctgtactcc ttcagcttcg
    23041 tttcaaagag ctcaaagagg gtgaacgcat cggccgtggt tccggcgaac cctgttataa
    23101 ccttgccgtc atagaggcgg cggaccttgt gggcattgcc tttcagcacc gcatcgccat
    23161 aggtgacctg tccgtcgccg gctatcgcca cgcggccgtc ttttcggatc gccacgattg
    23221 ttgttccttt aaattcattc atcgtttatt ccttctcccg tggggatgat gttcggccac
    23281 gaccttgctc aggcgcgctt gggaaacgtg ggtgtagatc tgggtggtgg aaatgctcgc
    23341 atgaccaagc agttcctgga ccagcctgat gtccgcaccg cggtcgatga gatgggtggc
    23401 gaagctatgc ctgagaacgt ggggagtgaa ctccttctgc catcccatct tcttcctata
    23461 cgcggcaaag atactgccga tcgtcgagaa cggcaactga tttcccgatg ttgaacagaa
    23521 caaatacggg gatgcacatt gattccgttt caccggcaaa tattcgttga tccgcgtgag
    23581 aaccttcttt ccgaagaaca cgaatcgctg cttgtttcct tttccgacga tcagaatcct
    23641 tcgctgcttc aaatcaagat catcctcttt gatcgaaacg gcttcaccga tccgacagcc
    23701 ggtgtcgtag agcagcgcaa agagcagaag atccctcatc gattcggcat cggacggctt
    23761 gagggcgagc agggcttgaa tctcatcccg ggtaaggatc gtcggaagac gattccgtgc
    23821 cttcctctgg acgatgaggg aaaaggggtt ggagtacacc cgttcctgct tcaagagata
    23881 gtcgtaaaag gttcgggcgc tgctgatcat ccgattcacg ctcgcctccg aaagacgccg
    23941 ggttttgctg aggtgtttca catacagcca gccgtcttcc aacgcggctt cttcaagtgc
    24001 gaccccttgt tgcctccaaa aggcgagcag ctgcatcaga tcacgccgat acgcgacgat
    24061 cgtgtgttca ctgaggttgc caacggtggt cagataccgg agatactgtt cgatgagctg
    24121 ttcctctatc atcctgttca ctataccaca accggctgat ccgggtcatc accgccgaca
    24181 attggtgctc cgctttgatg aaggaaccgg gagccttcct ggttgggttt gctcccgatc
    24241 ccgctttgat ggacaaagac atcctttccc aggtcgaggg cgcacccggc gacgtggagc
    24301 gctccgcttc tcttcggagc ttgaccgatg atgacggcgg tgcttaaggc ggcggtcaga
    24361 acgttccggg acagacagct ctgaggcgat acctcttcat acggcaaaaa cggcgagatg
    24421 agatgctcgc ccctaagcga tcgattgctc gtgattcgtt ttgttcccaa gccgcaatca
    24481 cagaggacga agctccgtct gtccgcgctt tgagaaccaa gccgagccca tcggtcaaac
    24541 ccgttgctgt tggagacgag aagggtgaac cccgtctccg caagagcttt tcccagagca
    24601 tacgcagccc gaagcccctc atagtcggat tgtctgctcc cggccagagc gatgatccgg
    24661 tcgcctgctt ctggcaggct tgcagagtag gaaagcataa aaggagggtt ctccaaatca
    24721 atgagggatg gcgggtaccc ggtcatccca taaaagacga tcttgtttcc cggtcgggaa
    24781 aagaaccgca acatcggttg aatgggaatc gaaggcgtga cagaggttcc ccccttggcc
    24841 agccgccgct tctcctccgt ggggaggttc aacgtggcga tcgacaacaa tctcgagatt
    24901 ctcatacccg ataagggtgg aacatcccca tgttgcttca atcggggtgg aactcctccc
    24961 agaaccgttg gagcgtggag gcatccacat gggtatagat ctgagtcgtc tggatgtcgc
    25021 tgtgcccgag aagctcctgc acggaacgca aatccgcacc actttggagc agatgggtcg
    25081 caaagctgtg ccggagggtg tggacgctca ttgtgaggcc tgccagggtg gcatactcat
    25141 caaagcgctt gctgatcgcc tgccgagtga gccgctgccc ccggcgaccg ataaagagcg
    25201 cccggctggg tttggagcgc gagagaagat ggggcctcgc ctcctgaaga taatacgcca
    25261 gcgacgcctg ggccacctcg ccgacgggga tgagccgcat cttgttgcgc ttgccgatga
    25321 cccgaagttg gcagtcctga tagtcttcaa tatcgatcga tgccgcttcg ctgattctga
    25381 gtccgcagga ataaatcact tcaaagaggg tcttgtccct gatgcccaga tagtcggcgg
    25441 tgttgatgac cgagaagaga tgttcgattt catcataggt ggccacctga ggaagcgact
    25501 cctccttctt cggagtccgg atgagcgaga gcgggttgtc agcgcggatc ttttgaagct
    25561 gaaggaacgt gaagaaggaa cgaagggcgc tctgcgtctt ggcgatgctt cgaggagaca
    25621 gcgatcgttg ctcactcgaa gcgatgatga acgcttcgat ctcttgaacg gtcacgagtt
    25681 cgggatcttc aaacgcttca agcaagaggc ctgcctcaaa gaggtagacc tcgattgttg
    25741 cctcgctgag ccgcttttga agagcaaggt agtgctcata ctcctccaag atcaaaaaag
    25801 cgcttcgctt accgctcctc ctcatctttg cctcggccat accgtaaaac ggttggaatc
    25861 gtatagtcgt cactgctggt cgaagcgccc tgtctcagct gtttccgcaa gtcctcccaa
    25921 cgaaacactt caatcgaagg cggttcgggt tcatgggcgt gctcccgctc ctgatgggtc
    25981 tgcggcgttg agaatcggcg tttgatcatc tccatatcgg tgttttcgac tccgacctgc
    26041 tcggtttttc gctcaaaacc cgtggcgacc acggttactt tgatctgctc gccaagctcg
    26101 gggttgtacg cttgcccggc gatgatcagc gcatcatcgg cacagttttc ggtaaccaat
    26161 tcgacgacat cctgatattc ctgcaaggtg aggctgtctc cgcccgaaag gttcaccagc
    26221 acgcttttcg ctccgtcgat cgaagcgctt tccagcaagg ggttgctgat cgcctgacga
    26281 gcggcatcga cggcccggtt ggcgccttca ccaaaaccga ttcccatcag cgcgtctccc
    26341 ttgcctttca tcaccgtacg aacgtcggcg aagtcgatgt tgatctctcc cggttcggtg
    26401 atcagttcac tgatcccctg cactcccatg tacaaaacct cgtccgccat cagaaacgcc
    26461 tgcttgatcg gggtgttgtt ctccacgacc cgaagcaggt attggttggg aataatgatg
    26521 agcgtatcga catgctttcg cagtttttcg attccggcct gagcgagagt cagtttcttt
    26581 tttccttcaa acgcaaaggg cgttgtcacg accgcaacgg tcagaatgtt catgctcttg
    26641 gaaagctcgg cgacgatcgg ggcggctccc gttcccgtcc cgcctcccat cccggcggtg
    26701 atgaagacca tgtccgcatc ttcaatctcc cgtttgatct cttccttcga ttcctgagcc
    26761 gccttttcac cgatttccgg aataccgccg gcgccgagtc cgccggtgag ctcctttcca
    26821 atgggaagac gaatatgggc gttcgagcgt tgaagagcct gcacatccgt gttcatcgca
    26881 acgaaatgca ctttcttcaa gccgctggct atcattcggt tcacggcgtt tccgcccgct
    26941 cccccgatgc ccaacacttt gatcaccgtc gcagtggtct gctccgatcc gataatatct
    27001 tctacgtcaa acatcccaaa atccatatgc attccttctt agaacaatgt cctgaaaaat
    27061 cccctcaatt tcgaggcggg tccttgtgtt cgtgccttgc gtcccacttt ccctgtcggc
    27121 tggttttccc gaagccgacg cgcttcgctc ttcaacaaac ccaacacggt cgtgtaatgc
    27181 gggttgatat agcttcgatc caaccccccg atcgcctcgg gaaaaccgag ccgggcgggc
    27241 atcctgaaca cctcgccggc aaggtccgta acgccgctta agagcgctcc tccaccgacc
    27301 agcacgattc ccccaccgaa gcccgagccc tcatgctcct tgctgaatgc atccaacaag
    27361 agggagaaga tctccgccat ccgcggctcg atgatccggc tcagctcgcg cttgggcatc
    27421 ttgatggcgg gcatcccccc cacttgagga atgatgatca tctcttcgtt cgaaacggaa
    27481 ggaacatagg agtggccgct ttcccgtttg atctgttcgg cgacagcccg ggtcttgttc
    27541 aagatgtagg cgatgtcatt cgttacatcg tcaccgccga ggttcacccc tccggtgaag
    27601 atcggcgctc cgtttcgata cgcgatcatg tcggtggtgc cgctgccgat gttgatcaga
    27661 atcgtgccca tctccttttc ttccgtggag agcacgactt ccgcatccgc caggctttgg
    27721 aggacgaccc gggcgacatt gagaccgctg cgctgaatgc acttgcgctg gttttgactg
    27781 atcgcgctgg aagcggttac gatgagcacc cggctttcca gccggtgtcc gagcatatcg
    27841 atcgggtctt tgattccgct ctgtccgtcg atctgaaaat cctgcacgag ggtatggaga
    27901 atctcgcgat cctgcggaag ctcgaacgcc cgggcgactt ccagactcct gaagatgtcc
    27961 ccccgcttga tctcctgctc ggggctgctg attcccacga cgccggttga aggaatcccg
    28021 atgatatgcc ccccgccgat gccgacaatt acgtcgctga tctccgctcc ggcttgaagt
    28081 tccgcttcat tgatgacggt ctggatggtt ttaagggtct gttcaatatt gacgatcgat
    28141 cccgatcgaa ctccctcgga gggatgttcg caaatggaat cgaccatgag ctggccttcc
    28201 cgattgaccg atccgatcac cgcccgggtt cggcttgacc ctatatcgag tcccatcaac
    28261 attttttcac ccgccattgt cgtctccttg gccacaccta tcgtcgcacc aacgcctctc
    28321 tatagaggtc gtaacgctga atgcttgaac tcaaaaagga aagattctcc tttcggtcct
    28381 gttcgatcca atagagcgcg gtctgaacgt gctttggatc caccggttcc cgtacccaaa
    28441 gccgggcgtt caacgaagga agatccaaaa ccattttccc aaagctatta ctgctattat
    28501 tatcgtattt tatacgagtt atcaaggagg cttcaccact gaggccccgg gtaagaccca
    28561 tcaccgaagc gaagacggaa tccgttccgt atcgttccat caacagggca tattcgaggg
    28621 gaacctcgat cctcaacagg tgatcggtcc aaagatccgc atcgctgctc tcaatcggaa
    28681 caagggtccg cccatcgacc agataggagc gttctttgtt ctcgctttga acaataaccg
    28741 gcgaatcgat gaaggcgatc gtcgctttga tggtcgacgg cagagttcgt gtaaggacca
    28801 atgaggttac gatcggcagc tgttcgatct gctttttcgt tgacaagagc gacactcgaa
    28861 agattccctg gcctacaagc gttgacaggc ggttggaaat ttctatggga acgtgcttga
    28921 gcgggccgtc gatcttcacc tggacctggg aaatcgtgaa gtaaggaagg tgggaaagcg
    28981 caagaaagag gagcaccgaa agaagcagag cggtgatgcc gtagatcgtt attcgtcgaa
    29041 tcatcttgct tcctcctttc ggtttgcatg cctcagcacg ttgagaatca aggcgctggc
    29101 gatgaggacc acgaacatgt tcgttccccc ctggctgaag aacggcatcg gaatccccgt
    29161 cggcggagcg agggcggtaa tcaccgccag attcaggatc gcttgggaga gaaccatcgt
    29221 ggtaacgccg tagctcagag cggaaagaag cggatcgcgc ccgatcaggc gcttcgctcc
    29281 gttgtatccc aagagcgcat agaggaagaa gaggaaaaga atgaagagga ttccgacaaa
    29341 tccgagctcc tcggcgacgg aggcgatgat gaagtcgctg tgcacttccg gcagaatccc
    29401 cagtttgtac gtccccttgc ccagacctgc gccaaagagc ctgcctttgg cgatcgcttt
    29461 caagctgacg ttcacctggt agttcattcc cgaaggatcg agagagggaa agaagaacga
    29521 ggccaccctg cgaatccggt aggcttggct gaagagggcg gcaaagagcg ggggaatgag
    29581 aaacaggagc aggagcacca cgttggcaag ccggactccg ctgtacagga gcatcgaaaa
    29641 ggcgatgaca acgatcagca gcattgtcga gtagtcccgc tgcagaaaaa ccagagccgc
    29701 atacagcaac gccactgccg ccggaaggag aatcttcttc ggtttcccct gataggaggc
    29761 aaagagaaga atcaatgcaa gcttggcgaa ctcacttggc tgcaatgcag gcagcggacc
    29821 gatctgaagc caccgtctgg agccgaactt ctcctgtcca tacggggtga ataccgtcaa
    29881 gagcagcaac gccaatgcag cgagggcaag gggccaggca aacgctttga gaacctttga
    29941 aggcaaaaag acgatgatta ccgagcctat agcggccaat ccgacgaatt gaagttgacg
    30001 aaccacatag taataatgcg gtacaccgtt gagcagcgct tcattatagg aggcgctgta
    30061 cagctgaacc aatccgaaaa tcgtcaaaag gagggttacg atcagaaacg tgacggtact
    30121 ctttgatttc ggctgctcga tcgatcccgg catgggatcg gagccctgat aatcaaagtt
    30181 ttgatacccg cttcccatcc ccttaccccc acatgacgaa ggagaggatt gcaaacaccc
    30241 atgacagggt tacaaaggtt tgaacaatct tcccctcggc gaatccggcc gcttccaagg
    30301 cgtggtggag cggcgcgatc aaaaagattt ttctaccaaa tccccgaatc gatataattt
    30361 gaacaagtga ggaaaagagc tccagaaaga acaggcctcc ggcaaagaga atcagaacct
    30421 cccggttggc caagagggca ccgacggcga tatacgcacc cagggcatga ctgccgctgt
    30481 cccccatgaa cgtttttgcc ggcggatagt tgtaccagac aaaggcgatg agagcccccg
    30541 gcaacgcacc gctgaaggct ccctgaacgg aaagcaggag aagcagggag ggaatcgccg
    30601 agaggctcgc cagcccgtca agaccatcgg taatgttcac cgcattgaca aacgaggtga
    30661 tgaacaagac cgcaaacggg taataccacc atccaagctc cagcgtcatc gatcgaaacg
    30721 tcaaggttgt tcgaaggggc atcacggcga caagcagaat cgaaacaaga agctgcaatc
    30781 cgagtttccc aagagacgtg aatccgtcgc ctctcctatg gcgttcctta agcaggtcat
    30841 cgaaaaagcc gattcctccg aaggtcaaga gagcgatgag cggataaaga atggtcggag
    30901 aaaaaaaatc ccgttcaaag agtgtggcga tcgaagaacc gatgatgaac gcgacgcccc
    30961 cgatgatggg agttccttct ttgtttgtgt ggaaatccgg tagatatgct tttgctgtcg
    31021 ttccctgacc ccggtacaaa gacaaaagaa ccttcgaaag gacgaaggta atcgtaaagg
    31081 aaaggaatac gacgacgatt cttctaccca tgcagggcga ccccccgttc gatcgtcgga
    31141 atcaggcgtt ccatttgcac gacccgtgaa cctttgagga ggacgaggtc accggcggtg
    31201 acctcgtctc tgaccatccc ttccagctcc tcaaagtctt gcgtgaagga gactcgctcc
    31261 ttacccttct ctctctttat ggtttgatac gcttcctcca tctcccgtcc gtagaggaag
    31321 tgtttcgtaa atgttgactg aatcaatgtt tttgcaattt caagatgggc ttgtttgctc
    31381 tccgccccca gctctttcat cggtccgaga accgcattct tccgtccgct ccatgagagg
    31441 cgggaaagat accggatgat cgatctggtt gaatccaaac tggcgttgta cgtatcgttg
    31501 atgatcgtca cgcgggatgt ccggacggtt gatcggccga ccagcggttc gaaatcctcc
    31561 agcgcatggg cgatatccac cggtcgggca ccgaggtaag cgccgactcg gatcgcacct
    31621 gcaacatctt caaggaggtg tttcccgacc gctttaattc gaaacgatac gccttggtag
    31681 gaaatctgcc atccgtcaag gccgaggtcg atggcgctca cgtcccgagt tgaatatcga
    31741 atcaactcaa cgggactgag ggtttgaatc agatgaccat acaggctgtg gcggctgatg
    31801 aaccccgctt cgatcccggg gtggaagatc ttcgcctttt cccgggcgat cacctcccgc
    31861 gatcccatcg attccaaatg ggcgttgccg atattggtca acaccccgat cgaggggctt
    31921 gccatcttcg tcatccgctc catttcgccg ggatggtcga tgcccagctc aaacactccg
    31981 tacttcgttt cggagttcag gttcatgatc gacaaagcca aaccgtattc gctgttgagg
    32041 ttgccgcttg tcatcgtggt tggcccaaga gcggaggcga tctttcctac cgcctccttt
    32101 gtcgtgctct tgccggttga accggtaata ccaattgtcc ggacatgagg aaagagtgct
    32161 cgataggcgg ttgcaagatt catcagtgcc tgaaacacat catcacttgc caacacggca
    32221 caggaggttt cattgagcgc tcgttgaata tcttcggtat ggacgagaac ggcgacagcg
    32281 ccgttgctct ccgcttcttt gaggtaatcg atcccgctcg agttcttccc tcgcagggtg
    32341 aaaaacaggg agccggaaac acaacggcgg gaatcaaggg catagtgggt tatcgcccga
    32401 tcggatgaac ggacgactga tgccttgctg tattgacccg ccaatgctat cttcatagat
    32461 cacctcgggc gacaacggcc gtgatcggag tgaagaagat ctcttcctgc caagagtagg
    32521 cgcttaccgc ttccgggatc gccttcgtgg aaatcgatgc tcggaggagc tgggtgcgtt
    32581 cctccagaag gatccggttg tcaaccagcg atcgatactc gatttccagc gaacgatgaa
    32641 tcccccgctg ccaaagcggc agaagcagca ccacgacggt aaagatgagc atcaacgcca
    32701 ccaagatgtg cttcactacc gaggatgacg gtttttcttc atactgcggc tgatcgaacc
    32761 attcatgctc catgacgctc ctcctcacgt attttttcca cgacccgaag tttggagctt
    32821 cgggacgccg ggtttcgggc aatctcctct tcaccgggaa caagcggttt tttcgtaaga
    32881 acgttcaata tcggccgatc ttcctgagca agattcttga acaaccactt taccggtcga
    32941 tcttccaacg agtggaacgt gataaccgcc agcctgcctc cgactttcag ggttctcagc
    33001 gcctcggtga gagccggttc gattcgatcg agctcatggt tcacttcgat tcggatcgct
    33061 tggaaggtgc gggtcgccgg atgaatcttt tgatagcggt acgccggagg gacgctcttg
    33121 tagatgatgt tcgccagatc atccgtcgtg gcgatggttt ttgtttttcg atgctggacg
    33181 atcgcccggg cgattcttcg cgagtagcgc tcttccccgt aggcgtagat tacgttggca
    33241 agcttcttct catcgtaccg gttcaccaca tcgaacgcgg agatcgcatc ttcactgtcg
    33301 agacgcatat cgaggggctc gcctttgcga agagaaaacc ccctttgcga ttcgttgaaa
    33361 tgaaacatgg agattcccaa atcgaaaaga atcagatcga aggaggaatc ctccgcttcc
    33421 ttgaagaaca catcgaacca catattgacc ggtttgaaac gagggccgaa ctcctccatc
    33481 cgaacccgtg ctttttcctg gatttcccga tcccgatcga ctccgatcac cttgaggttc
    33541 ggataggtgg aaagaaaaag gtgggtgtgc cccccttcgc cacaggtggc atcgaccatg
    33601 atcgggtcgg aactttgggg aacaaggtga gtaagcagct cgttgggcat gatcgaggta
    33661 tggacgtagt tcatcactcc tcctctctgc ccagattcga cagggactga gcggcatcaa
    33721 ggaactcatc catcgattcc tcaaggtatg cctcgtacgc ctctttgttc cacagctcca
    33781 gatagttgcc cgctcccaga agaaccgatt cctctttcaa gagcagtttc acgctttcac
    33841 ggagggaagg gggaatgttt atccgaccgg ctttatcaaa ttcacagagc tgggcgggag
    33901 cgatcatcct ccgctgaagg attcgaaggc gccggtcgaa catcgagtgc ggaccgttga
    33961 tgatcgtgtc tttcagctgt tcaaaatctt tggggagcat caaccagaga caggtttcca
    34021 gaccgcgggt tacgtagagc gcatcaccgg agagggcatg gcgaagccgg gctgggataa
    34081 gtagcctacc cttgtcatcg agggtaatgt aaaactcgcc ggttaccatt gaaacacccc
    34141 ttaatatggg aaaatttccc actggtatcc attaatgtac acttcttccc accggggtgc
    34201 aaccatgttt aatctcttac tggcagtcac attggtgatt tttcacggca accgcttaac
    34261 atccgtttag ataattgata catattgtca ataacgacca aaaacgcctc cttcaacgag
    34321 gaggcgttcc gcaggatcgg atacttgtca aattcgttcg gaattgaaga tattgtagtc
    34381 aatgtccccg aagattgtgt gacgtttttc cgctttcacg agccactcgg tatcgacggc
    34441 attcttgcac atgtcgtcat agacgaggtt cacgttcttc aggtgtcctt cgattcgttt
    34501 ctttgcgtac tcgacactgg ttcggcagta aatgatgaac ggccaatcgc tggacatcgc
    34561 cagaagcacc tcccgcgctg cttggttgag aaaccgccgc ttaagacttg tctggtcggg
    34621 gaaccgggtg gcaagctctt ccatccgggt aatcgcttta tggatgtgtc gataatattc
    34681 cgtgtttgat ccgtccagcc aagcggcgct gtaaccgccc tgcccccagg aggagaacgc
    34741 cggccgcacg gtttgcagcg gggtcgcatc ctcggcaagg aaggacgatg gagttaccag
    34801 ctttgttcgg tctttgctgg ttcgaatcac ctcttccagc caatcgattc cttcatacca
    34861 ccagtggccg aagagctcgg cgtcaaagcc gaggaagaac aacggctgtg ctcctcccat
    34921 cgtagcgcgg aggcgatggt tcctgttgtt gacaacatag tggaagttcc tcgcatgttc
    34981 cttgacgcgt tccatcgctt gagcgcgaac ataagggact ttttgatcgg tatcgccggt
    35041 aatcgcccag tatttgtacc cggtgaagac ccgcacttcc ggttcatgga tgtagggccg
    35101 gatatagtcc atattcaagt cgtatccgat atcccgataa aactcgcggt acacgttgtc
    35161 cgttggataa ccggtggtgt tcgaccacac cagactggtt gcctgataat cgcgggggaa
    35221 cgcagcaacc ccactgggac agcggaccgg gcgatagccg ccgtaggtgg ccttatccgg
    35281 tgaaagcagc atgctttgac tggcaacctg gaaccatttg atcccatggt acttcagatg
    35341 ttcttcaaga ccggggtaat atccgcactc gggcaaccaa aaacctttcg gaggatgtcc
    35401 gaacgtcgtc aaaaagcttt ggacgcccaa ttcaatctga gcattgatcg cctgtggata
    35461 ttcctcgtag aggggaagat acgcatgcgt tgcggcggtg gtgatcaatt ccaaatgtcc
    35521 ggattgctca agggctttaa agccgtcgag gatgttcatc cgatagaggt cctcatactc
    35581 atgaagattt cgaatgtatc cgtcaaggta atgttgggcc atctcaagaa attgaggctg
    35641 ctcatcttta cagcgttgaa cctctttttg gcccagctcg atatgttgct gcaggtagtg
    35701 gacaaaacgg ttctgcaggg cattgtcggt gagcatcgct atcaaaggcg gcgaaaagct
    35761 tatggtaagc tgaaagctca acccctcttc cttgaggcga ttcagcatcc gcaaaagcgg
    35821 caagtagctt tcactgatcg attcaaacag ccaatcctct tccaaaaaac gggggtattc
    35881 gggatgtcgg acaaacggca gatgagcatt gagaacaaag gctacgttgg cgttgctcat
    35941 gaaagcaccc ccttgctgac ggtgagggca agatctttaa ggacgacgtt ttcggcaacc
    36001 tctccggttt tggtgaccag cgatgagaaa tgaagcagaa agaggtctcg atccatcgcc
    36061 agctcttcaa tccgatccgc ccaatacggc aaataggttt cgacgctcgc cgaacgggcg
    36121 acgacttctt catttccgtc gggatggagg cggcataatt caacactgta ggaacaaccg
    36181 agcgccggaa ggtggatatt ccactcctga tcctttactt ccacggcgat atcgctcgag
    36241 gtcgtcgttc cggtagccag ttcggtctgg tgaacccgaa tcacatagga ttcgccgctg
    36301 tggtcttcat catcgaagga ggttttgttt ccctgaatcg tttccggcag ggaccagtac
    36361 gcatacgccc attgagggtc gcgcaagagc aggtgaattg tagaatcata ataggaagga
    36421 ggcagctcgc cggtccgcgg aagatctttg aagtgagccg cttttccccg gtggttggtg
    36481 agattattgg tgtaacggcc gtaggtaccg tgcccattgc ctacggggat gccgccttcg
    36541 tccatctctt caaaccaatc ctccaatgca ctgatgagct catctcgatc cagtgtcttc
    36601 caacccttca gtccttccat ctgtgcgatg tgctgcagtt cattggtgga taacgaatca
    36661 atgttgataa gaatcatgaa cgctcccata aatctgatgg tgaataccta cgcaatcgta
    36721 gaagaatcat caatcttagt caaccacacc tgtatatcag ccccttggat acttcgaacc
    36781 acaagggtat attgagcaat aattcatatc ccggagaatg attatatcac tgagagatta
    36841 cagacaaggg ctgtgccatc gacacagccc tttacatgac acgaatgcgg tcttagaaca
    36901 cggatcccgc gtcgtagtgg tccatcacgt tggatttgtc aaccaacact gatggcagaa
    36961 caacggagag aggagctttt gccgtgtgga acgcatcact ctttcggcct tcgacgacat
    37021 cgaggcagta catgatcgca tcatacacca ttgaggggtg atacgtagcg gtcgccttta
    37081 cgagcggatc gtcgttcatg atcatctcat aggcggcctt gcttccggcg ccgccgaaga
    37141 caagcttgat atccttgcgg ccactttcct tgatcgcctg cagaactccg gtcattacgt
    37201 cgtcatcctg gcagaatacc gcatgaatcg tcggatactt ctggatgtag gtttccatca
    37261 gttccagacc cttctgggtg gaccagtcgg cgaagtcgta gttccgtccg ccggcaaggt
    37321 tgacgagcgt gggctctttg gtcatttcat caaagaatgc attcatgcgt tcgccatcaa
    37381 tgacgaccgg cataccgccc atggcgacat atcgggtcag tccattcgct ttcatgaagt
    37441 cgcgcatgaa gattccgctc accttgccca tctcgctgtt gttgccggca aggttcacat
    37501 acccgaatga cgtgtcggtc aaaccccggt caacgacgat gcacttgacg ccggcagcgt
    37561 gtgcttcttt cacaaccgga gtcagcggag cgctttcgtg gggaagaatg acaagataat
    37621 ccatccccca gaccatcagg tcctcaacat cggagatctg ctttgcatac ccgtcggcgt
    37681 gcaagacccg gaactcatac ttgccgggat actgcttctg aagctcatcg accgctttgt
    37741 cggcccacca gccgattccg ccggtccagc cgtggtcggg agaaggtact gcgattccga
    37801 tcttcggaac tgcggctttc tcggctgcac cagcggcgaa gacgactccc atggcaaata
    37861 ccaaagagag aacgattact aaagcttttt tcatacatag cctcctgttt atttgttact
    37921 gtcgctgttg tactgaagca gtactgcgac aataattaca aaacccctga ctgcttgttg
    37981 caggaaagcg ctgatgcctc ccatgacaag catgttgttg atgataccga gcatcactgc
    38041 ccccatcatc gtaccgatga tgctcccctt ccccccgctc atcagcgttc cgccgatgac
    38101 cgccgcggca attgcgtcca tctcgtagcc ggcgcaatcg ctggggttga tgcagttgag
    38161 cctgcttgac cacatgatcg ccgtgacacc gacggtgaat ccggtgatca cgtacgggac
    38221 gaatttcacg agagagactt tgatcgccga gtatcgggca acctgctcgt ttgcaccggt
    38281 cgcgcagaga taccggccga agctcgtgta gttgagaatg atatggagtg caaccccgat
    38341 gatgatgaaa aaccatacgg gcaccggaag accgagaaag atgctgcttc caagctttcc
    38401 aaaccccata ttcactgaaa gaatcgttcc ggcgtttgaa aagtattgga tgagcgatct
    38461 gaagatcgac atcgtaccca gcgttacgat gaacggggcc atcctgaact ttgcaaccat
    38521 cactccgttc agagctccca agagggatcc gaagaccaga gcgaagaaaa acgtgaggac
    38581 cagcgcaagc gtcgaatcgg ggccgaacag gttgaggaag aagattgaaa ctccccccac
    38641 gaaggccgcc atcgacccca ccgagagatc gatgccgccg ctgatgatga cgaatgtcat
    38701 ccccaggccg atgattcccg tataggaaac ttgccgaaga atgttggtca ggtttcgaac
    38761 cttgagaaag ttcggccaac cgacgatggt tgcaatcacg aacaaagcga tgaacgccat
    38821 tcccagcgag tggtctttga tccgatagtt cttaatgtat tctttgatct tatgctcagc
    38881 catctctctt gacccctttt tgtatcccgg ttgcattgaa tacgatattc tcctcggtga
    38941 tgtgctcctt ctccagatgc ccggcgattc ttccttcccg catcacgtac acccggtcgc
    39001 acatcccgat cacttcctcc atttcgctgc tgatcacgat gcaactgatt ccttcatcag
    39061 caagatggtg gatgaattca tagatctccc gtttcgcatt gacatcgatg ccgcgagtgg
    39121 gctcatcaag aatgagaacc cggggtttcg tgtcgatcca ccgcgccaga tacactttct
    39181 gctggtttcc cccactgaag aaacggagag gcgctttgtg cgaagccgcg acgatgttga
    39241 accctttgac gtattccttg ccgctctttt cctcactgtg cttatcgatc aaaccccgtt
    39301 tcacgtactt ctctttgagc gaaatcaggg tgatattctt gggaatgtca tagtccatga
    39361 cgattccctt gccttgacgg tcctcgctga tgtacgcgag cccgtggctc accgcttcac
    39421 gggggttgct gatcttgacg ggctttccgt caaatcgaat cgtccccgaa ctcatctttc
    39481 tcaagcccat gatcgtttcc atcgtttcag tccttccggc tccgaccaac ccggcgaacc
    39541 cgagaatctc ccctttgcgg agggtgaatg atacatcacg gaggagatcg gggcaggtaa
    39601 ggccttccac ttccagaagc ggcggttttc ccgtcgaatc gatcttgtcg ggaaacacct
    39661 gactgaaatc acggccgacc atctttcggg cgatgatcgt ggtatcaaga tcttttatct
    39721 cgtcgacgga aacaagatgc ccgtcacgca ggatagtgac gcgatcacag attgtcatca
    39781 cttcaccaag cttgtgactg acgaagatca tcgtgacccc ttgctccttg agcttgcgca
    39841 tcaaagcgaa gaggtgttgc acttcaacgc cggtgagggc ggtcgtcggc tcgtcgaaga
    39901 tgagaatccg agcatcaagc accatcgctt ttgcgatttc aaccatctgc ttttcggcaa
    39961 ctgaaaggtc gctcaccgtt cgatggacgt cgatcggcat cttcagcgat tcaagctggg
    40021 ctttcgcctc ttcccgcatc ttgggcctat cgaggaacac tcccttcttt cgctcattgc
    40081 caaggaagat gttctcatat accgtgagat agttgatcag gttgaactct tggggaacca
    40141 ggccgatccc caggcttttc gcggtgatgt agtcgggaat acggatatct tttccgtcaa
    40201 gacggatcgt tccgcttgtt tcctcataga ttccgcagat gatctttatc agggtggact
    40261 tgccggctcc gttttcaccg acaagcccca tgatttcacc cggatagatc gacacattca
    40321 cctgatcaag gacccgaacc gttgagaact ctttaacgat cccttccaac tccaaaattg
    40381 gtggacgctt tgtcattaat tctccattgc tccatcaaaa tcaattgtgg aaggggcaaa
    40441 atcgattcgt cgaaccaact ccaacgcctc ccgggcgcca aagagccgat ccatcccgtt
    40501 gtcttcccat tcaacggaaa gcgggccctg atatccggcg gcgtttgctt cccggatgat
    40561 catatcgaag ttgacgtccc catgccccgg cgagacgaag ttccatcccc ggcgcagatc
    40621 gccgaacatc agatgggatc cgagaatacc gcttcgtcca tccagcttca ccacggaatc
    40681 cttgatgtgg atgtggtaga tcgagtcggc aaaatccctg aagaaaagcg ccggatcgac
    40741 cccctgccac tggaggtggc tcgggtcgaa gttgattccc aacgtttttc ttccgccgaa
    40801 cacctcgaac agctttttcg tcgaatagta gtcgtaggcg atctccgagg ggtgaacctc
    40861 gagggcgaac ttgacgccct gcttgtcaaa ctcatcaaga atcggggtcc acagcctgag
    40921 aatctcctga tatccctcct cgatcagctc gggagtattt gccgggaagg aatagaagta
    40981 tttccagatc ggcgagccca tgaaaccggt taccacatag caacccatgt ttttcgccgc
    41041 ctgagcggta tacttcatct gctcgatacc ccaggcgcgg atcgcttcgt cgtctcccgc
    41101 caacgctttc ggtgcgaatc ctgcaagacg ggggtctccg aacgcatcac cgacgcactg
    41161 gccggcaagg tggttgccga gggcccagca gccaagattg tatttcttca gcgtcgccag
    41221 cctgtctttc acgtatgcgg gatcggtggc cgctttcttg acgtccatgt ggtcgcccca
    41281 acaggcgatc tcaagtccgt cgtaccccat ttcactgatg atcttacaca cttcctcaaa
    41341 gggaagatcg gcccactgac cggtaaatat cgttacgttc ctagccattg tatctctcct
    41401 ttttacattt caacccagag cgcgccctgc ttgctgcttt ggacgcatcg attgatgaaa
    41461 cgaactccat caaggccttg atccactgat gggaaatcca gatcggaagc ttccagtttt
    41521 tctcctctca gatgttttgc caatgcggta acaaagggaa ggtaaatatt ggcaaatgct
    41581 tcatagaccc cttcagggtg accggaagga attcggctgt atcgttgagc ctcttcggca
    41641 aacgggtcgc ggccgcggct gaacacctgc tttggagcat cgcttggagt aaagatgaaa
    41701 tggtcgggat cctcctgagc ccactccaat ccgccctttt caccgaagac tcggatccga
    41761 agggcgttgt catagccgaa ggcgacctgg ctggaccaat acatcccttt tgccccacct
    41821 tcatactcca caagaaccgt agcgttatca tccagcgtcc gcccctcgac aagggaatca
    41881 aggcgcgcac agacccgttt gatcttcagg ccggtgacgg tggaaacgag attttcgatg
    41941 tgactgccga tatcgccgag acaattggaa atgccggaga cttcgggatc catccgccag
    42001 ggggcgatcg gacttgactc atcgacggga ttggcgagcc aatcctgcgg gtactcggcg
    42061 tttacaaacc gaatctttcc caaaagaccg tcgcggacca ttttccgcat ctgcttcacc
    42121 gcaggatatc cggtatacgt gtacgtaacc gcaaagagaa gattcttctt ttcggccaag
    42181 cgctgaagct cttcactctc ttcgattttc cacgtcaacg gcttgtcgca gactacatgg
    42241 atgccctgtt ccag
//
LOCUS       DAOSLI010000011        40414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1141_length_40414_cov_24.721227, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000011 DAOSLI010000000
VERSION     DAOSLI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_40414_cov_24.721227"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..377)
                     /gene="ssrA"
                     /locus_tag="PLF67_02935"
     tmRNA           complement(1..377)
                     /gene="ssrA"
                     /locus_tag="PLF67_02935"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(435..1196)
                     /locus_tag="PLF67_02940"
     CDS             complement(435..1196)
                     /locus_tag="PLF67_02940"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOE89005.1"
                     /translation="MFDKKFNEFISLLERSKSTVVLTGAGVSTFSGIPDFRSSDGIYS
                     KQYKHLSVEEIISLPLFERDPEIFYSWAKDEWYNLADFEPSIVHIALAKLEEAGKLDG
                     LYTQNIDMLHTRAGSRKVYELHGSAAHHHCTSCRASYPFDEIYATVKSGSVPKCTKCG
                     GVIKPDIIFYGENLDNSLLTRAYNAFSKADLAIALGTSLIVQPVASLVYTTISRGGEL
                     VIVNRQSTPYDRNATVRFDDLKEFSEALLAYLKAR"
     gene            complement(1189..2490)
                     /gene="miaB"
                     /locus_tag="PLF67_02945"
     CDS             complement(1189..2490)
                     /gene="miaB"
                     /locus_tag="PLF67_02945"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOE89006.1"
                     /translation="MKRKYWLETYGCQMNIAESNALELHLKGAGLIPAESAEEAAVAI
                     LNTCTVRRSADNRIWGRLGYFGALKKRQPLTLIVTGCMAERLKDDLKNDAPYVDFVIG
                     TNDKQRIVDILTSPNGAYDEEETYTFSESHYREGEFSSYIPIMNGCNNFCSYCIVPYV
                     RGREISRPINDIIRELHYLDSKEVKEVTLLGQNVNSYHYEEDGKVITFPGLLRHLCAE
                     ETKHIRWIRFDSPHPKDFTGELIEVIASEEKVAKHLHIPLQSGSTRILKAMNRKYTKE
                     QFISLITTIRSAIPSVTFATDVMVGFPSESEEEYRETLEVLRLLQCPEAFMYYFNPRE
                     GTKAVELEGQIDEETKSRRLSELIDFQLEIFQTVRRARIGAILEVLVSQQSRDDEHRM
                     LGKSEHNEMVAFDSDAPIGSFVTVRLTGLKGNTLQGEVVHV"
     gene            complement(2468..>3208)
                     /locus_tag="PLF67_02950"
     CDS             complement(2468..>3208)
                     /locus_tag="PLF67_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOE89007.1"
                     /translation="MKEKACDRLGFDHATYRFDESVSEATLLSLLSKLNDDPMVDGIL
                     VQLPLPDHLDTTKILDTISPQKDVDGFHPINVGNLLLGNPSLVPCTPLGILKMLEYYE
                     IETSGKHVVVVGRSHIVGKPIAALLFQKGWDATVTICHSKTQNLRDITSLADILIVAI
                     GKPLFITPDYVKEGAVIIDVGINRVEDAGRSRGYRLVGDVDYERVAPKTTAITPVPGG
                     VGVMTVAMLMNNTLQAAKKGRNEAEILA"
     assembly_gap    3209..3308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3309..3839
                     /locus_tag="PLF67_02955"
     CDS             <3309..3839
                     /locus_tag="PLF67_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89008.1"
                     /translation="PFYDKGSQTFSFTLGTTVPTFVHFFSNNETRVGLGVDNTGMKIG
                     GYGAISYQAFTSPYSAIGGEIGYNFNYVVDGNLYTAVPFFFKYSYLPVQGMIDLPISV
                     GLGFVYNSGGANTSIMTLYSNLELGLTYYPGEHWGIGVRTGLWLIPELNYRKELFGDN
                     AIAGFVPITLTVSYRQ"
     gene            3849..4976
                     /locus_tag="PLF67_02960"
     CDS             3849..4976
                     /locus_tag="PLF67_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89009.1"
                     /translation="MKKILIIMTAITLLFTACNADATSGLFRTAAEAREALTIKYMQV
                     VGRKGANDTLYFLTREGLFVKDTNAKAAELIVKSVEDNRVQSAAYLNDKIKLLYKTDK
                     NDGSLFEYDPVTKASTPLPIPAAPVTDFRLMPNGKIVLKGAANSGEFWIVANDKLGSY
                     NADGIKVTAGEGFDFYGLFTPSNYEKSDANKGFLLSFVKSGSPNEYKNYYYDGAGVNE
                     MNNTLGSISNFSVIGTNLYILTVEGALYHLKTGALNVAAKLKDNTVGYERNAFFYSID
                     DGANLHFITNPSSSDSMSVFSVVKGDPKADTTTVSTIKNGYAVVLKDTFIVSSLQLDG
                     ETFLVATEKHGMYSVKVDKVNVNKNDGTNVQAISKAEEYEF"
     gene            complement(5038..5652)
                     /locus_tag="PLF67_02965"
     CDS             complement(5038..5652)
                     /locus_tag="PLF67_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOE89010.1"
                     /translation="MNKEFINCDTIRDNTLKLAHHMYKNDGFIPDIIYASLRGGAYMA
                     NVFSEYYKMVKLNRKERPVFYAAVVARSYGYLRNQSRVMVDGWTYSPEFLRSGDKILL
                     VDDIFDTGKTVNALGEIIMQKGIPRNDLKIVVYDYKVPLYKKGEPLPIQPDYYVRKHV
                     LHSEEEEVWIHYNCHEFLGLSSQEIDEQFQDPEVRTILHEVRND"
     gene            complement(5659..6414)
                     /gene="map"
                     /locus_tag="PLF67_02970"
     CDS             complement(5659..6414)
                     /gene="map"
                     /locus_tag="PLF67_02970"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOE89011.1"
                     /translation="MIQLKSEEQIKRIGEACTLTARLMEELETFIEEGMSTKDIDQYC
                     HDFIIRHKGIPSFLNYGGFPASACVSVNEEVIHGIPSTKKIIREGDLVSVDLGINLNG
                     YFSDMARTFIVGETKEEYRKLCEVTRKSLDLGIEAAGKNGARIQDIGKEVSRHCSQHG
                     YGVVRDFTGHGVGLAVHEAPEIPNYSAPFLPNPRIREGMVLAIEPMINLGTHKVSILE
                     DNWTVVTQDGLPSAHFEDTVAITRDGLVILTRG"
     gene            complement(6395..6853)
                     /gene="nusB"
                     /locus_tag="PLF67_02975"
     CDS             complement(6395..6853)
                     /gene="nusB"
                     /locus_tag="PLF67_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOE89012.1"
                     /translation="MRSRHKARELAIQTLYAMDFNDDLNVSNIPTTFSAVTDDEYESF
                     EEDVRIYAMYLIRGTLEHLEEIDHLISRFSLNRPLDRIDYVDRNILRISVFSLLYANV
                     HPHVIIDEAVKLSQDFSSEVNYKFINGILDAMNKQVFEKREGMNYDSTEE"
     gene            complement(6858..7889)
                     /locus_tag="PLF67_02980"
     CDS             complement(6858..7889)
                     /locus_tag="PLF67_02980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE89013.1"
                     /translation="MKRFASLTILILMTMSLVFAATISSPAATVRLTKTVQISMNDLD
                     AEVARYQAATSDPSSVDPLQVLNLLINNELFRQGAERDGVVISSTMVETAYKNQKTAI
                     EQSYGTTLTNEEFDQIIVNNFGSVAAYKQLISEQLLVDSYVQLKKSDVLKEEPIVAES
                     EVTSFYRKNRTQFVSPETVKLSHIFIPYSSDSSVNTANKTLLADVANKIKNGTLSFEK
                     AVVEYSQDTPSKSKGGDIGWLTMDNEEARLGLGDSFFEAAFSLDVGETGGLVESNTGY
                     HILKVLVYSEPKILALDDVISPNNPMTIRTYITNELKVAKQQELYVKAVNSLVEDLRK
                     SATVRILYK"
     gene            complement(7940..8353)
                     /locus_tag="PLF67_02985"
     CDS             complement(7940..8353)
                     /locus_tag="PLF67_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89014.1"
                     /translation="MNVGILYGSSQKNDGKMKAIAQGLSEGIQSQGHRVDLIDMNLEV
                     GKIVSFYDYLVIGTNNTTFFGGKIPEVVPTFLKGAGTLSGKRCMAFITGGGVRTMKTL
                     QTLMKAMEKEGMFLKKSEVIKKPDLAKAIGKRIQI"
     gene            complement(8438..8749)
                     /gene="secG"
                     /locus_tag="PLF67_02990"
     CDS             complement(8438..8749)
                     /gene="secG"
                     /locus_tag="PLF67_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOE89015.1"
                     /translation="MGVLSVIILILFIIVSLLLIFLVAIQDEQSEGLGGIFGGGSQTA
                     FGSQTSSVVTKATGILAALFLVLALLVAFINKTPSQDRLLDSVTTEQVQQTTEWWDAG
                     N"
     gene            complement(8766..9518)
                     /gene="tpiA"
                     /locus_tag="PLF67_02995"
     CDS             complement(8766..9518)
                     /gene="tpiA"
                     /locus_tag="PLF67_02995"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOE89016.1"
                     /translation="MKQAYIAGNWKMNMTPSEGAAFAKELVKAIKNTNSKVMVAPPFV
                     TIPAVLEAAKGSSLIVAAQNMANHESGAYTGEVSPLMLKDLGVNDVILGHSERRSIYG
                     ECDEVINEKLLLALKHDMNVVFCIGETLEERESGRLEEVLERQVRVGLGGVKADQMGK
                     VTIAYEPVWAIGTGKTATPDDANNAHAFVRSVVEKLFSKGVAEATVIQYGGSVKASNA
                     KALMAMPHIDGALVGGASLSVEQFLPIVNFDK"
     gene            complement(9521..10699)
                     /locus_tag="PLF67_03000"
     CDS             complement(9521..10699)
                     /locus_tag="PLF67_03000"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011772017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOE89017.1"
                     /translation="MTLQTITMCDCTNKKVLIRVDFNVPVKDGNVTDDTRIRAALPTI
                     NALLDKGASLIVMSHFGRPKGKKNPEFSMAPIAKRFEELLGKKVTLAGDVIGPEVEAA
                     VNALKKGDVLLLENVRFYAEEEANDSEFAKTLASYGDLYVNDAFGTAHRAHASTEGVA
                     HFLPSYAGLLIEKEVKFMAPLLSNPEKPFVAIIGGSKVSSKISVLESLLKTCDAIVIG
                     GGMAYTFLAVQGHAIGKSLVEEEYKETAASFLKQAEKQNVRVILPVDHLCGSEFDEKT
                     PAIVVDGVDIPENLIGMDIGPKTVKLITDALQGAKSIVWNGPMGVFEFDTFAKGTLAV
                     AKALAESKAITVVGGGDSVAAINKFDLADKISHVSTGGGASLEFLEGKTLPGIKALEK
                     "
     gene            complement(10766..11770)
                     /gene="gap"
                     /locus_tag="PLF67_03005"
     CDS             complement(10766..11770)
                     /gene="gap"
                     /locus_tag="PLF67_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006434126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOE89018.1"
                     /translation="MKIAINGFGRIGRNVFKIAFVDKDIEIVGINDLTDPKTLAHLLK
                     YDSNYGVYDKSVAVDGDAIVVDGKKIPVFAERSPANLPWKDLGVDVVIESTGVFTVAE
                     SPKGGYKDHIKAGAKKVVLTVPAKDKIDQTIVLGVNDDQLDLSHEAFSNASCTTNCLA
                     PIAKVLDDNFGIVKGLMTTVHAYTNDQVMLDQPHKDLRRARAGAVSIIPTTTGAARAV
                     SEVIPSLKGKLNGMAMRVPVPTGSVVDLVVELEKDASVEEINAAMKAASEGSMKGVLE
                     YTEDPIVSRDIIGNHHSSIFDAQLTMKMGDKLVKVLSWYDNEMGYSHRVVDLVKKLVK
                     "
     gene            complement(11845..12609)
                     /locus_tag="PLF67_03010"
     CDS             complement(11845..12609)
                     /locus_tag="PLF67_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE89019.1"
                     /translation="MKRSLTSLFRRKAANEPLPYQELEERKEMIEGIVELKELTVKEV
                     MVPRVDVQFIDSTTSLEDLYSIIEEQGYSRYPVYEQTIDNIVGVLYAKDMLRREIPNP
                     FDVKQLMRKPYFIPDSKHLDDLLREFKKRKVHIAIAIDEYGGVSGIICMEDILEVIVG
                     EIQDEFDEDEDEEIRRIDEHTYLIDARTSIDEVNEVCSLDLSDEEFETIGGYVFELFG
                     RIPKKGEEIEENDALFIIDDIDGHKINEVKLILKRS"
     gene            complement(12620..13054)
                     /gene="ybeY"
                     /locus_tag="PLF67_03015"
     CDS             complement(12620..13054)
                     /gene="ybeY"
                     /locus_tag="PLF67_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOE89020.1"
                     /translation="MEPYTIDIAVDDGMAVDTESVEATLIFTLNYLRVGPCEVSVSFV
                     TDEAMQQLNNRYRNLDEPTDVLSFAQEEGGESFGPVRILGDIVISLDTMERNALLFDV
                     EPSIELQRLLIHGVLHLLGRDHATNEEREEMLLLQEKILGHL"
     gene            complement(13054..15234)
                     /locus_tag="PLF67_03020"
     CDS             complement(13054..15234)
                     /locus_tag="PLF67_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOE89021.1"
                     /translation="MRKLSRQRSAGSRYQPLIIIMLMILVTGCAFFIPLLPHISSASK
                     QFTYQVGQLAEEDIFAPRDFIHVDAEKTEERRLAAERAILPIASLNLRQTSLSYTRLD
                     IFSKLLLSETDQRESNLYTFLGSENLVDRRNLLQRLFLLKEAELAYYVATLNETASMV
                     LSRGLMDHDSYERITSDGYDSFHLEMGVQSHGDESDSLDEVLTKENLDDLLFAYLRGY
                     IRQQSSFQPQLIIDTLVLLLENNLIYDEAKTLSLRSQARAKVELVQIAIPKNTKLVSK
                     DTPLTANQVRMLETMNEYSFLYTPTQLLARAIFTIMVTLVGCRAFILFAQGHFRSNLY
                     LILALSVLLISVLLIGLIGWLMPGTVLDSHLPVLLAPMFILQITDKKRLAFITSLMIS
                     SYVALLPTSSMMTFFFSIATGSLVLWTFQSAQRRLDTLSNWAYGAITTSFAALVANML
                     VGLDSHLYLPLVFGMIINVTVSMILVEVSVPIFERLLNIPTPYRLMEISREDNPLLDR
                     MAQVAQGSFTHSRHVAELAYVAAKTVGANPLLARVGAMFHDIGKSDHPEYYIENQTEE
                     NKHDDIKPSLSVAIIKSHVKLGVEKGREAGLPQEVLDIIGQHHGNDVIQFFYSEAIKE
                     AQERSGEVNIEDYSYNNEIPQTKEAAVVMLADVVEAATRTMKKPTHSKYQKQIRKLIM
                     GKVDRGQLKDSGLSLTDLDRIEEVFLQQIIGRDHHRIEYPQEEE"
     gene            complement(15235..16194)
                     /locus_tag="PLF67_03025"
     CDS             complement(15235..16194)
                     /locus_tag="PLF67_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOE89022.1"
                     /translation="MEKRITFDSEELVRRVLGINDRNIPYMETLLGCELFVRSNDVIC
                     LSDDPLIQNRFLLLFGRLSRLAEHQLFLNETDIFMEFQSIKNDVSQDRDVVNGDRENR
                     KTIKIHNRRAYAKGANQERYVELMEENKIVFAIGPAGTGKTFLAVAYALSQLFSGRKR
                     KIILTRPIVEAGESLGFLPGDLAQKINPYLRPLYDAMELLISPANIRKLEDGGNIEIA
                     PLAYMRGRSLRGATVILDEAQNTTVEQMHMFLTRLGEDSKAIVTGDISQIDLPKSKMS
                     GLVHAEKILQGIDELAFAHFTSSDVVRSRIVQAIIDAYQNGDA"
     gene            complement(16210..19086)
                     /locus_tag="PLF67_03030"
     CDS             complement(16210..19086)
                     /locus_tag="PLF67_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89023.1"
                     /translation="MKKARVASSFCFLVFLLALCVNGSLFGRESGQEYILNIINADGF
                     ESLEGGRYLLKGNVHLMIEEEGSEEKIRLYSDRLLVDVSDKSLTAMGSVRTEGAKEIS
                     GEIITLMYERGDIFASCADLVTISEELGDQPVTLHTLGTRITIQSASSMITYTDARIG
                     TRAIDPLSSIRAKRISVLNDGDIIAHGATLSIGRVPLFWTPFLFIPGSRMIANPAMGF
                     ISNRGMFVNTTWEIFGRYPHFRKDEQRSLATLLSAKEEAGAIRAWPIYTLEKAPSSFE
                     QWARESSSYLAIFGDAYEDTGVSLGYDSRIKLLKNTLTINSLSTVALDPSGIDQRVAW
                     SDVNKVRMWSMNTLFYRVGSSSLTATLPYYSDPSVERLYSSRLHNLRLDALFGSVQEF
                     PSLGSERSSYTTGLTGNLTIPTTIFKEYLSSLTIDNIALQGTYRWQKKDGIHAYHLTE
                     VTLPRFIIRASGTLLNLAGQTTVKSPPVSNTPDVNPLLPNLYRPQRQQPASLFSRQAE
                     RSLKLSYTISEEFARTYNKTAGDDPNVYSMTRGTLILSAVPDSRFFTSSFELLPQLSV
                     SDDPQKTTNHTQVFQLHANTTLHLPFIGVSYTLRQRLYRQEETQSGDKPKNVVIDRFR
                     FDEKSVSTHQLRFDRTYRLWAGTINPSITAVLWPLTRTLAPKVIVRYQGASLTTSLRF
                     KDASGTGFLEKELFSLGVGYSDSPLVTNTTFSYNYLKPLEPSRLDHQLTLSLFDKELV
                     LSERFEYFPMKNGVEHVLSHGVLGLKTSFLTLNYHAGGTLGNIQSERLTAMVSLAERQ
                     WRFYKKRIALTLGVNAAFDYDVDDPFSSVFTFSLKSRFQIAEFLDLSIALASSNTGFH
                     HYQDDAGSFAWLLLWKDLLRSFDLFGGGIHHTQFNLSSVDIQLIHYLEDWRLNCKYSG
                     SVVLSNNQYRWVPTFSIYLAWNTIPDLDIEEKWSRTGGVWDRSL"
     gene            complement(19073..21910)
                     /gene="uvrA"
                     /locus_tag="PLF67_03035"
     CDS             complement(19073..21910)
                     /gene="uvrA"
                     /locus_tag="PLF67_03035"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOE89024.1"
                     /translation="MQNKLVIKGAREHNLKNIDLTLTKNQLIIISGISGSGKSSLAFD
                     TIFAEGQRRYVESLSSYARMFLDKLDKPDVDYMEGLSPAIAIEQRSTQRNPRSTVGTV
                     TEIYDYFRLLWARVGTPHCHVCHRKISEMSVDQIIDAIFKAKDQSRIIVSSPVAIGKK
                     GEFRKVFEDAKAQGYHRLVVDGQQIDLEDGIPALDKQIKHSIDIIVDRLILSADIRTR
                     LASSIETAIEMTQGLVKITFLGEAGSSHEEIYSERNSCPHCGITLPELEPRLFSFNNP
                     FGACPECNGLGFKTEFDPDLIIPDYSKSFNEGAIATMNPDAQWSRSQFSALAKHIGIT
                     LDTPFSELNEEQLQAILWGTEEQVMVEYVRDKGNRTIHVAKPYPGVIPDLQRRYYETP
                     SMHIRTWMNGFQTTKVCPACSGERLRPEALAVTVQGLSIMEATRQSVAQSKEFFDTIA
                     LNESQTVIAAQVLKEIRSRLLFLRDVGLTYLTLDRSSATLSGGEAQRIRLATQIGSAL
                     SGVLYVLDEPSIGLHQRDNQKLIDTLKRLRDLGNTVLVVEHDEATIREADWVVDLGPG
                     AGIHGGRIIAQGTPQEIEANPASITGQYLSGSLVIEIPKERRSGNGEILHIGGANKNN
                     LKHIDVDIPLGKLVVFTGVSGSGKSSLLNEVLLPAVRRTLAKKQPNYKGYSSISGVEH
                     IDKVINIDQTPIGRTPRSNPATYVGLFTPIRELFASLPESRARGYKSGRFSFNVPGGR
                     CENCKGDGTLKIEMNFLPDVYVTCDVCHGKRFNQETLAVRYKGKNIHDVLEMSMGEAS
                     EFFNAIPKIKRKIDTVISVGLEYIKLGQSALTLSGGEAQRVKLSLELSKLSTGKTLYV
                     LDEPTTGLHFADVKKLLEVLNRLVDQGNTVLLIEHNLDVIAQADHLIDLGPEGGDGGG
                     MVVASGTPEQLALCKDSYTGYYLAQMFHEKG"
     gene            complement(21900..22946)
                     /gene="ispG"
                     /locus_tag="PLF67_03040"
                     /gene_synonym="gcpE"
     CDS             complement(21900..22946)
                     /gene="ispG"
                     /locus_tag="PLF67_03040"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOE89025.1"
                     /translation="MNDVHLVHIGNLTIGKGKPILVQTMYDDALPVDMKRAEALLIRI
                     GKLNTIGCDIIRFSYPSKDDHEVFKYLAKASPIPIVADIHFDHTLALDAIEAGAHKIR
                     INPGNIGARWKVDEVVRAAKDRDIAIRIGLNSGSLPRREAASPAHLMKDTALEYLDWF
                     ERLGFTNTVVSLKGSDTDLTIEANRLFAKESSYPIHLGVTEAGSVISAVTRSTWALGT
                     LLSEGIGNTLRISITGEIETEIQAGVELLRTLGLKKKGIRVVSCPRCGRHTFDTIGFL
                     SSVEEELLAIEKDLTVAIMGCQVNGPGEAKEADVAITGIGNAIFLYEKGVLSRKVTAQ
                     DAKEALLEAIRNAE"
     gene            complement(23019..23375)
                     /locus_tag="PLF67_03045"
     CDS             complement(23019..23375)
                     /locus_tag="PLF67_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89026.1"
                     /translation="MIYDTLDSLDHYAHLFIVDKPVYEPHHPEPFDGMFTAHSHWGTV
                     FLVKEGEVLACSTHARQPGTLLRDINGFVHHESSGITSTARVDANHFIFFHPYEPYAL
                     IVEKEAAVARLLVEVR"
     gene            23440..23895
                     /locus_tag="PLF67_03050"
     CDS             23440..23895
                     /locus_tag="PLF67_03050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOE89027.1"
                     /translation="MKMTALYQSPLGPLYFIEEDERLVEVGFGERAETSTTPLLREAM
                     RQMDAYFNDTLTRFDLPLHPRGTEFQLKVWEALQTIPYGQTRSYRDIARIVGNVKAVR
                     AVGGANNKNPISIIIPCHRVIGKSGKLIGYGGGLEKKAWLLAFEKRTPS"
     gene            complement(23932..24369)
                     /locus_tag="PLF67_03055"
     CDS             complement(23932..24369)
                     /locus_tag="PLF67_03055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269054.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOE89028.1"
                     /translation="MVNMEFIGMAAALTLSALGSGIGAGIASMAAVGGWKKCYVENKP
                     APFIMVAFAGAPLTQTIYGFLLMNFIANAIAGGIASTLALFVGVLGGLAIGLSAYMQG
                     KAAAAACDALAETGKGTANYFIVIGIVETVALFTLVFSLLALQ"
     gene            complement(24409..26229)
                     /locus_tag="PLF67_03060"
     CDS             complement(24409..26229)
                     /locus_tag="PLF67_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOE89029.1"
                     /translation="MKKTYIVVQRSKTKAMLKNLRKAGVLHVSTASKAFDGSYKQEIE
                     DVEKVISVLQELVDKKQPAAQKTLSRREVVETTAYLISLLNKQNELIQKRDRDSLSAA
                     TLLPWGDFDPEELAWLKREGIELFFYTIDKKDLAKLDEEQVYYEVSYRGPMKAIATIG
                     EQLDPSTGAVPATFAKGRLSDLQRSIDQAGKELVRIDEKLKASLVHLDALKHYRSVLE
                     MRTRFEEVEASLVDDEELSYLVGYLPTKEEEQFTRLAKKNGWAYLLEDVSEDDEDVPT
                     LIEYRKGVGIIKPVFDILGTVPGYREHDISTWFLLFFTLFFAMIIGDAGYGLIFLLIA
                     GAIHISMKKANTLVMLVYVLSIATIVWGSLTGTWFGSIEILQSIPFLQKLVIPQISNY
                     PELFGIEAVTAQNTVMKFTFIIGTVQLSLACIINVVRKARIKDLSLVADLSWLIALLA
                     LYYIVLLLVIGAQVNIKALFATVGVAFVTILIFGAQGPGVSFIDGIKGGLAGFFTTFL
                     DTISAFSNIMSYIRLFAVGMASVAIAQSFNAMASGMLKGFALPAGILVLVIGHGLNLV
                     MGLLSVVVHGVRLNLLEFSGQLGMEWTGIQYQPFAETVEE"
     gene            complement(26243..26842)
                     /locus_tag="PLF67_03065"
     CDS             complement(26243..26842)
                     /locus_tag="PLF67_03065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOE89030.1"
                     /translation="MAVKLTKNELKLQKDRLKQFERYLPTLMLKKQQLQLVIQQIERE
                     IEEHKQKQAALIAQVKTWIAVYAENERYDLLSLVQIDTILKSRSNIAGVIIPTFKELT
                     FKHIEYNPLHYPLWIDKGVSFLREVAQYDALIATLNEQIELLGRELRITSQRVNLFEK
                     VKIPEAKEHIRLIGIYLGDQQTAAVVRGKIAKKKLVAGV"
     assembly_gap    26956..27055
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27056..28311)
                     /locus_tag="PLF67_03070"
     CDS             complement(<27056..28311)
                     /locus_tag="PLF67_03070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOE89031.1"
                     /translation="MQKIYSKIESIVGNVITVRAEGVANGELAVVSSSGSTSFANVIK
                     LDGDLVSLQIFSGATGTSTGDHVRFLGNPMRVSSSPNLLGRVFDGTGSPRDGKPELKE
                     EMIDIGGPSVNPAKRIIPRNMIRTGIPMIDLFNTLVESQKLPIFSVSGEPYNELLARI
                     ALQAEVDLIILGGMGLKFDDYLYFKDTLEEGGAMDRTIMFVHTASDPTVECMLVPDMA
                     LAVAEKFALEGKKVLVLLTDMTNFADAMKEMAITMDQVPSNRGYPGDLYTQLASRYEK
                     AVDFEGAGSVTILAVTTMPGDDVTHPIPDNTGYITEGQYYLKGGRIEPFGSLSRLKQQ
                     VNSATRDDHRALMDGMIRLYASFKEALEKQSMGFMMSEWDEKLLKYGDLFEEKMMDLS
                     VNIPLEEALDLGWEILGDCFEPNETGL"
     gene            complement(28323..30044)
                     /locus_tag="PLF67_03075"
     CDS             complement(28323..30044)
                     /locus_tag="PLF67_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOE89032.1"
                     /translation="MARNTGRVLGVNGNMVNVEFDGAIAKNEVGYIHLGDIRLKGEVI
                     RINGNIAALQIFEMTGGIVVGDEVEFTGEMLSVELGPGLLTQIYDGLQNPLPELARQC
                     GFFLQRGVYLQALPNVDWEFTPTVSKGDIVKAGDTIGTVPEGLFTHQIMVPFDLVGSF
                     TVTSIKQAGTYKTRDIICTVEDSKKKKLELSMVFSWPVKKAIRLYSERLRPVEPMMTK
                     IRIIDTLLPVAKGGTFCTPGPFGAGKTVLQHMTSRNAEVDIVIIAACGERAGEVVEIL
                     KEFPELIDPKTGRSLMERTIIICNTSSMPVASREASVYTAVTIAEYYRQMGLDILLLA
                     DSTSRWAQAMREMSGRLEEIPGEEAFPAYLESRIAEFYERAGIVKLPDGRTGSVTIGG
                     TVSPAGGNFEEPVTQATLKVVGAFHGLSRERSDARKYPAIDPLTSWSKYPGIIKQSEV
                     NQMRRVLHGSHEVNQMMKVVGEEGTSINDYVTYQMGELLDSCYLQQNSFDPIDTSVPP
                     ERQKHVFAKLHRLMNTPLTFETKSDVRRVFNNLRQLLLDWNNTRWGSDEFKAAEAALD
                     EAFKALG"
     gene            complement(30056..30589)
                     /locus_tag="PLF67_03080"
     CDS             complement(30056..30589)
                     /locus_tag="PLF67_03080"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOE89033.1"
                     /translation="MASYYYFATTLPLLRLHQKSPITVELFKAQAEKHLNSRDWKTLK
                     SGLSGTPSKHALLTSYRSYASALNTALAKKRQVRLGFAIDEDLHRQTFPKEIETVTDE
                     LLERSDPLEAEMQFITLLWKRIDRLVGMKVFEFDALLGYGLKLLLLERVNIFTQEVGQ
                     ATFETLLGTLRSEIDQM"
     gene            complement(30599..31183)
                     /locus_tag="PLF67_03085"
     CDS             complement(30599..31183)
                     /locus_tag="PLF67_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOE89034.1"
                     /translation="MAQQIQDLVASIRKDGVEAAKKEAASILQSAEEQAQTIIAQAQE
                     KATHIVSEAEEAIALANRRAEKALSQAGRDLLLSLKASIEGQFSRILVEKTSKALSSK
                     DFIGLLTKVLEAGDGDVNIEIDPKAFEALADELQTALAAKLKSGLVLKPHDDVESGFR
                     LVAKDGSYFYDYKASELADLLQPYLARPLHERIF"
     gene            31305..31694
                     /locus_tag="PLF67_03090"
     CDS             31305..31694
                     /locus_tag="PLF67_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89035.1"
                     /translation="MYYHAVKKSSEVLYRTKEEAQRLLFALHAKLTKQHATILDYLLE
                     PQTCQLLLQSKKPIILPAFTINPVEKEKLLWYFSSLGSKGKTYPYSGLHECYFLSTCF
                     CELGKVTADPPPYPLKEVLAVKHGRAE"
     gene            complement(31672..>32012)
                     /locus_tag="PLF67_03095"
     CDS             complement(31672..>32012)
                     /locus_tag="PLF67_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOE89036.1"
                     /translation="AIALGKHPHTGVVQCKDSFYGQHDPSKMPVFYELENKWEAYKRL
                     GVLASEMESAALFTVAAYLGVRVGSLFFVVGNQEREKAGLPNPIVHDTSEVIKISVEG
                     IRTLILRDRA"
     assembly_gap    32013..32112
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32113..32603)
                     /locus_tag="PLF67_03100"
     CDS             complement(<32113..32603)
                     /locus_tag="PLF67_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOE89037.1"
                     /translation="MSDYTHGSGIQYHLGTKKGDVGRYVIMPGDPKRVPLIAKHLTDA
                     RPVADVREYVTYTGYLEGEKVSVTSTGIGGPSAAIAMEELYKSGADTFVRMGTCGGID
                     PKVIGGDVVIATASVRAEGTSREYAPIEFPAVATFEVVEALRDAAIALGKHPHTGVVQ
                     CKDS"
     gene            complement(32626..34944)
                     /locus_tag="PLF67_03105"
     CDS             complement(32626..34944)
                     /locus_tag="PLF67_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOE89038.1"
                     /translation="MTGKSLDDLEFAKVQTMISSFRLSEEGGEFLASLPFAMEQEELT
                     LRQGYISRILGSFERDGVPALAHFPSIADVMDRLGEPTPSPDGPGLLDLARYLQSSEE
                     LFDYLLRDEEEALRELIGLGVDPALIALKEAILADLDESGSVRENHPRLQPLYRQLEQ
                     VRQKRASYCSSFIRDNRSSVQSDVEALRDGRLVIPIKDGYKHNVKGYISSVSSSGQTV
                     FMEPFALVDLNNSVLLANNRILVEIAKILQELALKAKQEEKLLHELRDTVGTADGYYT
                     LSRWAHEQRCTIVRLDSDEVRLLDARHPLLKQKAIPITIILEKGINGVVLSGPNAGGK
                     TVTVKTFGLFALLNQYCGYLPVREGSALPLFDRVFTDIGDEQSIEKELSTFSGHMSRL
                     AEILSHLTPNSLVILDELGSGTDPLEGSALAQAILSYSLKKSALTLVTSHHGVLKEYA
                     YSKENIINAAMEFDEVSGKPTFRIVPNVIGQSHALVTAQAMGLPEEVLSEARELLGSD
                     RIELSSILMRLEEERKRVEERSKALSKIESRWKEEYRKLDLKALTIKQQERQLKDEEI
                     RTLARFTQKARQRLEQLVTDIREGELTGEKTKAVKTYLAELDRRVETEEAALEKTEEA
                     FVHPTTFEVGMEVLCGPSKREGTIIKKEGRNRYLVAIGSMRMSLKESDLYLPRNRDST
                     VKVSYTANARRAKQEIDVRGFTLEEALEAVEQNIEAALLNNLSTFGIIHGYGDGILSQ
                     GIGRLLKNHRSVVDYRFALPEDGGMGKTYVFL"
     gene            complement(34925..35893)
                     /gene="rsgA"
                     /locus_tag="PLF67_03110"
     CDS             complement(34925..35893)
                     /gene="rsgA"
                     /locus_tag="PLF67_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOE89039.1"
                     /translation="MEERTGLIIRGINNIYTVESGEEQYLCRIKGKQLEGVEGEYNPL
                     AVGDRVSFVVTGTEEGLILRRLQRTNSFQRWNTKGKANQTVVANMDAIICVTSAANPP
                     FRPRFIDRVIASAIDVEVIILLNKCDLEMKEEDAFRFEHYGALGFQTAAVSAETGENL
                     DRLVQIIRDKKVAFVGQSGVGKSTLINALLGSDQKTDEISTKYDRGRHTTNHSLLMRT
                     GNLTIVDTPGVREIFVPHTDLQQIQRAFPEFKRYRCLYDPCLHMEEPECGVKVAVEEE
                     EILFDRYESYLRIVESLEMITPDYLSRNERSEAWIERGAEYDWEES"
     gene            35957..36514
                     /locus_tag="PLF67_03115"
     CDS             35957..36514
                     /locus_tag="PLF67_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOE89040.1"
                     /translation="MYSFTRCKVSSCPRYATHISEYCLAHDPRQDLAPTLSFPVLDSA
                     SLSNWNCTEEDLSGKRIPGSLFSYSTFHGVSFVKTTILNSNFSFCLFEECVFDESTIR
                     YVIFSGSTFLGCTFLNSSITHTNFNGSVMRDCDASGSDLYYSSFAHAHIHNTKMEDCN
                     VRKADFRHTLQDNLSFRWSNYREVL"
     gene            36514..37059
                     /locus_tag="PLF67_03120"
     CDS             36514..37059
                     /locus_tag="PLF67_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE89041.1"
                     /translation="MRIHQHFSVVGFCNTYVIGDNATKDALLIDPGHADIELIRLLEQ
                     HGYTIKAVLLTHRHSGHTEGLGLLKKLYDFTTYAYEDPRHPIIPNEILTIGPFEIFSY
                     LVGGHSRDSVVYKIKDALFVGDALRAAALGKTHTHRLAEHLKDELNRIFSSLDPQTLI
                     FPGHGPPSTIQSELLFNQSLR"
     gene            complement(37044..37952)
                     /locus_tag="PLF67_03125"
     CDS             complement(37044..37952)
                     /locus_tag="PLF67_03125"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE89042.1"
                     /translation="MAKITKTASFHVSDLEGKQRLDLYLASQNEELTRTIVANAALWL
                     NGKPAKKGNPVQTGDEIRIEYTLTVFDGLTPQQIDFSILYEDDSILVINKPQGLVVHP
                     AAGNWDNTLANGLLHRYGEEILAVQDEHGDGVRPGIVHRLDKETSGVMVVALTQAAHR
                     ALSAQFKDRSTSKIYIALVAGKVKNAKGTIDLHIRRDPSNRKKMAVCSDDNGRQALTE
                     YTVLRQFRDCALLYLVLHTGRTHQIRVHLNAIGHPVVGDPLYGREVEEGMMLHAKSLA
                     FAHPVTGERMTFTAPLPRRFFDYLKL"
     gene            complement(37952..38278)
                     /locus_tag="PLF67_03130"
     CDS             complement(37952..38278)
                     /locus_tag="PLF67_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89043.1"
                     /translation="MKKPIIIFMILLILVPASLFSEPLKDYEPYEEGEFPLWTYNIRR
                     AETIFFGSLVITLPLSILLHSAARSAGIIPPQTSAMNDFLTQAAIAGTLSLGVSIADW
                     ALGLKQ"
     gene            complement(38280..38951)
                     /locus_tag="PLF67_03135"
     CDS             complement(38280..38951)
                     /locus_tag="PLF67_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOE89044.1"
                     /translation="MTALAEIRQEIEEAAGKSGRSLTDISLMAVSKTQPYERILDLYD
                     EGQLLFGENRVQEAADKLPGDRPQGMNVHLIGHLQSNKAKRALTLFDRIDSIDSLRLA
                     RILEKQLDRPYPILLQLKTSDEESKYGFADEDELLFALEAISSFRYLTVKGIMSIGPL
                     TSDETVTRKAFEQTRKLYHRAQELFPDLNLDTLSMGMSGDFVLAIAEGSTMVRIGTRL
                     FGART"
     gene            complement(38970..39041)
                     /locus_tag="PLF67_03140"
     tRNA            complement(38970..39041)
                     /locus_tag="PLF67_03140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39007..39009),aa:Val,seq:tac)
     gene            complement(39083..>40414)
                     /locus_tag="PLF67_03145"
     CDS             complement(39083..>40414)
                     /locus_tag="PLF67_03145"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOE89045.1"
                     /translation="AIMQSVSGEKMAIALAREGGLSFIYSSQSIQDQEAMVRRVKGYK
                     AGFVTSDSNLTPNHTLGDLIALKEEKGHSTIAITDDGTSNGRLLGLVSSRDWRPSRTS
                     PDTLLKDIMTPFESLIYGFEGITLSEANDMIWDNKLNTLPIIDKDRKLKYLVFRKDYE
                     SHKKHPLELLDKKKRYLVGAGINTRDFQQRVPALVDAGADVLCIDSSEGHTEWQKITL
                     DWIRSTYGETVKVGAGNVVDRTGFRFLAECGADFVKVGIGGGSICITRETKGIGRGQA
                     SALIEVARARDEYFEETGIYVPICSDGGIVFDYHMTLALAMGADFLMLGRYFARFDES
                     PTEKVLIKGQYMKEYWGEGSARARNWQRYEGEGQLSFVEGVDSYVPYAGKLRDNVQLT
                     LSKIRSTMCNCGSLTIGDLQKNAQLTLVSATSIVEGGAHDVLLKESDEYHR"
BASE COUNT         8739 a        10979 c        10603 g         9793 t
ORIGIN      
        1 tggaggtggg gggattcgaa cccccgtcct aatgtgccat ccaatgacac ctacaagctt
       61 agtcggtggt tttgttctcg aagagcaggt acagccaccg acctccatcc ggctcttcca
      121 gcctacttga ttgtcccctt cccccatagg cgttgagtcg ggtaagccct tgttgttaca
      181 ggtaaccgag ccttaagagc ggcaggttcc cggccctgtt cgctgtcgga ctgttaggcc
      241 gcgagagcga attctgcttc ggcgacgaaa tcttcgtctt cacgtttggc agttaaattg
      301 gttgctagtt taaggagttg gcgctccgct tgcagccatt gacctgatct accaccagtc
      361 gaaaccggaa cacccccgag gtattctggt gtttttaagt atactgaagc ggattacaaa
      421 cgacaagtgg tcgatcagcg ggcttttaga tatgcaagaa gcgcttcgct gaactccttc
      481 agatcatcga agcggacggt ggcgtttctg tcgtagggcg tgctttggcg attcacgatt
      541 acgagctccc ctccccgact gatcgtggtg tagaccagcg acgccaccgg ctgcacgatc
      601 agggaggttc cgagcgcgat tgcaaggtcg gccttggaaa acgcgttgta cgcccgagtc
      661 agcagggagt tgtccagatt ctccccatag aagatgatgt ccggcttgat gacccctccg
      721 cacttcgtgc atttggggac ggatccgctt tttaccgtgg catagatctc atcaaacgga
      781 taggaggctc tgcaggaggt gcaatggtga tgggccgcgc tgccgtggag ctcgtacacc
      841 ttcctgctgc ccgccctggt gtgaagcata tcgatgttct gggtgtacag gccgtcaagc
      901 tttcccgcct cttcaagctt agccagggcg atgtggacga tcgagggctc gaagtcggcg
      961 agattgtacc actcatcctt cgcccaggag tagaagatct ccggatcccg ttcaaagagc
     1021 ggaagggaga tgatctcctc gacgctgagg tgtttgtatt gttttgagta gataccgtcg
     1081 cttgaacgga aatccggaat tccgctgaac gtcgacaccc ccgccccggt caggacgacg
     1141 gtggatttcg atcgttccag cagcgagatg aactcattga acttcttgtc aaacatgcac
     1201 gacctctcct tgcagggtat tgcccttcaa gccggtcagc cggaccgtga cgaatgaccc
     1261 gatcggcgca tcggagtcga aagcaaccat ctcgttgtgt tcgctcttgc cgagcatccg
     1321 gtgttcatca tcgcggcttt gttggctcac cagcacctcc agtatagcac cgattcgcgc
     1381 tcgtcgtacc gtttggaaaa tctccagctg gaaatcgatc aattccgaca gtctccggct
     1441 cttcgtctcc tcatctatct gcccttccag ctcgaccgcc ttcgtgccct cccgggggtt
     1501 gaagtagtac atgaacgcct cggggcactg gagaagccgg agaacctcga gggtctcccg
     1561 gtactcctcc tcgctctccg agggaaagcc caccatcacg tcggtggcga aggtaaccga
     1621 agggatcgcc gatcggattg tggttattaa agaaataaac tgctccttgg tgtatttacg
     1681 attcatcgct ttcaggattc ttgtcgaacc gctttgcaaa gggatatgca ggtgcttggc
     1741 caccttctct tcgcttgcga tcacctcgat cagctctccc gtgaaatcct tcggatgggg
     1801 gctgtcgaag cggatccagc ggatatgctt cgtctcctcg gcacagagat gtcgaagcag
     1861 tccggggaac gtgatgacct tcccgtcctc ttcgtagtga tagctgttca cattctggcc
     1921 aagcagggtc acttccttta cttctttaga atcaaggtag tggagctccc tgatgatatc
     1981 gttgatgggg cgcgagatct cccgcccccg gacataagga acgatgcagt aggagcagaa
     2041 gttgttgcag ccgttcatga tcgggatgta ggaggagaac tcgccctccc ggtagtggga
     2101 ttcactgaac gtgtaggtct cctcttcgtc ataggcgccg ttcggagaag tcaggatatc
     2161 cacgatccgc tgtttgtcgt tggttccgat gacgaaatcg acatatggag catcgttctt
     2221 caagtcatcc ttcaggcgct cggccatgca tccggttacg atcaaggtca gcggctgtct
     2281 tttcttcagc gctccgaaat acccgagcct cccccaaatc cggttgtcgg cggatctgcg
     2341 gaccgtgcac gtattgagaa tcgcgacggc ggcttcttcg gcgctctctg ccggaatgag
     2401 accggcaccc ttcaaatgga gttccaacgc gttggactcg gcgatgttca tctggcaccc
     2461 gtaggtctca agccagtatt tccgcttcat tgcgcccctt ttttgccgct tgcaaggtgt
     2521 tgttcatcaa catcgccaca gtcatgactc caaccccgcc gggaaccgga gtgatggcgg
     2581 tcgttttagg agcgacacgt tcatagtcga cgtcgccgac cagccgatag ccccgtgacc
     2641 gaccggcatc ctcaacccga ttgattccga catcgatgat taccgccccc tctttaacgt
     2701 agtcgggggt aatgaagagg ggttttccga tcgcgacgat caggatgtcg gcaaggctcg
     2761 tgatgtcacg aaggttttgc gttttcgagt gacagatggt aaccgtggca tcccatccct
     2821 tttggaacaa caatgcggcg atgggctttc ctacaatatg tgagcgaccg accaccacga
     2881 cgtgttttcc tgacgtttcg atctcatagt actcaagcat cttcagaatg cccaacggag
     2941 tgcagggaac cagggaagga ttccccaaca gcagattgcc gacgttgatc ggatgaaacc
     3001 cgtcgacatc tttttgagga gagatcgtat ccaaaatctt tgtggtgtca aggtgatccg
     3061 gtagcggcag ctggaccaga attccgtcga ccataggatc gtcgttcaac ttggaaagca
     3121 gggaaagcag cgtcgcttca ctgacggatt catcgaagcg gtaggtggca tgatcgaagc
     3181 cgaggcgatc gcacgctttc tctttcatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnncc gttctatgac aaaggaagcc aaaccttttc ttttaccctc ggtacaaccg
     3361 ttccgacgtt tgtccatttt ttcagcaaca atgaaacccg agtaggtctc ggtgtcgaca
     3421 ataccggaat gaagatcggc gggtatggcg cgatcagcta ccaggcgttc acgtcgccgt
     3481 acagcgcgat cggcggcgaa atcggataca acttcaacta cgtggtggat ggcaatctct
     3541 acaccgccgt gccgttcttc ttcaagtaca gctaccttcc cgttcaggga atgatcgatc
     3601 ttcccatcag tgtcggtctt ggcttcgtgt acaactccgg cggggccaac acctcgatca
     3661 tgaccctgta ttcaaacctc gaactgggat tgacctacta ccccggcgag cattggggga
     3721 ttggtgttcg gaccggatta tggctcattc ccgaactcaa ctatcgaaag gagctcttcg
     3781 gtgacaacgc catcgccggc tttgttccca tcaccctcac ggtaagctac agacagtagg
     3841 aggaaacaat gaaaaaaatt cttatcatca tgacggcaat aaccctcctc ttcaccgcct
     3901 gcaacgccga tgcgacgagc gggctcttca ggacggcagc ggaagcacgg gaagcgctga
     3961 cgatcaagta catgcaggtc gtcggaagaa aaggtgcgaa cgatacgctg tacttcctca
     4021 ccagggaagg gctttttgtc aaagatacaa atgcaaaagc agctgaattg atcgtcaaga
     4081 gcgttgaaga caaccgggtt caaagcgccg cctacctgaa tgataaaatc aagcttctct
     4141 ataaaacgga taaaaatgat gggtcgttgt tcgagtatga tccggtaacc aaggcatcca
     4201 ctccgcttcc gattcccgcc gctccggtca ccgacttcag actcatgccc aacgggaaga
     4261 tcgtgctgaa gggggcggcg aattcaggtg aattctggat cgtagcgaat gacaaattag
     4321 gtagctataa cgctgacggt ataaaggtta cagcaggcga gggttttgat ttctatggtc
     4381 tcttcactcc ttcaaactac gaaaagtcgg atgcgaacaa agggtttctg ctctcgttcg
     4441 tgaaatccgg ttcgcccaat gaatacaaga actactacta tgatggagcg ggtgtgaatg
     4501 aaatgaacaa cacccttggt tccatctcca atttctccgt catcggaaca aatctgtata
     4561 tcctgaccgt tgaaggggct ttgtatcatt tgaagacggg cgccttgaat gttgctgcca
     4621 agctcaaaga caatactgtc gggtatgagc gcaatgcctt cttctactcc attgatgacg
     4681 gagcgaatct ccacttcatc accaacccct cctcttccga ttcgatgagc gtcttcagtg
     4741 tggtcaaggg ggatccgaaa gcggatacga ccacggtgag cacgatcaag aacggatatg
     4801 ccgttgtctt gaaggacacg ttcatcgtga gctccctgca gttggatggt gagacgttcc
     4861 tggtcgccac cgaaaagcac gggatgtatt cggtcaaggt tgataaggtt aacgtgaaca
     4921 agaatgacgg cacgaatgtt caggcgatca gcaaggcgga agaatacgag ttctgatgat
     4981 tcattggttt ttaaaggata acccccttct tttgtttgga agggggtttc gttttcatca
     5041 gtcgtttcgc acttcgtgga ggatggttct tacctcggga tcctggaact gctcatcaat
     5101 ctcttgggag gagagaccga ggaactcgtg gcagttgtag tggatccaga cttcctcttc
     5161 ttcgctgtgg agaacgtgct tgcggacgta gtagtccggc tggatgggaa ggggttcccc
     5221 ttttttgtag agcggaacct tgtagtcgta gaccacgatt ttcaggtcgt tgcgggggat
     5281 ccctttctgc atgatgattt ccccgagggc gttgacggtc ttcccggtat cgaagatgtc
     5341 atcgacgagg aggatcttgt ctccgctgcg aaggaattcg ggcgaatacg tccatccgtc
     5401 caccatgacg cgtgattgat tcctcaggta tccgtaggat cgggcgacga cggcggcata
     5461 gaagaccggt cgctcttttc ggttgagctt caccatcttg tagtattcgc tgaagacgtt
     5521 cgccatgtac gccccaccgc gcagcgatgc atagatgatg tcgggaataa agccgtcatt
     5581 cttgtacatg tgatgcgcaa gcttcagcgt attgtctcga atcgtgtcgc agttgatgaa
     5641 ttccttgttc atgtgaacct accccctggt cagaatgact aagccgtccc ttgtaatcgc
     5701 tacagtatcc tcaaaatggg cggaaggcaa cccgtcttgg gtgaccaccg tccagttgtc
     5761 ctccaggatc gagactttat gggtaccgag attgatcatc ggttcgatcg ccagcaccat
     5821 cccctcccga atccggggat ttgggaggaa gggggcgctg tagttgggaa tctccggcgc
     5881 ttcatgaacc gcaaggccga ccccgtggcc ggtaaagtcg cggaccactc catagccatg
     5941 ttgagaacag tgccgggaaa cctctttacc gatatcttgg attcgagccc cattttttcc
     6001 ggcggcctcg attcccaggt cgagcgattt ccgggtcact tcgcacagct tccggtactc
     6061 ttccttcgtc tcgccgacga taaatgtccg ggccatatcg ctgaaatatc cgttgagatt
     6121 gattccgagg tcgacggaaa ccagatctcc ttccctgatg atctttttcg tggagggtat
     6181 tccatgaatc acttcttcgt tcaccgagac gcaagcgctt gcgggaaatc cgccgtagtt
     6241 cagaaaggaa ggaattccct tatgccgaat aataaaatcg tggcagtatt ggtcgatgtc
     6301 tttcgtgctc attccttctt cgatgaacgt ttccaactct tccatgaggc gggcggtcaa
     6361 ggtacacgct tcgccgattc gtttgatctg ttcttcactc ttcagttgaa tcatagttca
     6421 ttccttcccg cttttcgaac acctgtttgt tcatcgcatc cagaataccg ttgatgaatt
     6481 tgtagtttac ttcgctggag aaatcctggc tgagcttcac cgcctcatcg atgatcacat
     6541 gggggtggac gtttgcatac agaagggaaa atacgctgat tcgaaggatg ttgcgatcga
     6601 cataatcaat ccgatcaaga ggacggttca aactgaatcg actgatcaga tgatcgatct
     6661 cttcaagatg ttcaagggtt ccgcgaatca gatacatcgc atagattcgg acatcttctt
     6721 caaagctttc atattcatcg tccgtcaccg cactgaaggt ggtcgggatg ttggatacat
     6781 tgaggtcatc attgaaatcc atcgcataaa gcgtctggat cgccaactca cgggctttat
     6841 gtctgcttct catgcttcta cttgtacaga atccgaaccg ttgcgctttt cctcaaatct
     6901 tcgaccagcg agttgaccgc ttttacgtac aactcttgct gtttggcgac tttcagttca
     6961 ttggtgatgt aggttcgaat cgtcatcggg ttgttggggc tgatcacatc atcgagggcg
     7021 aggattttcg gttcgctgta taccagtact ttgagtatat gatagccggt attcgattcg
     7081 accaaaccac cggtctctcc gacatccagg ctgaacgccg cctcgaagaa cgagtcccca
     7141 agaccgagcc gggcttcctc attatccatc gtgagccacc cgatatctcc gcccttgctt
     7201 tttgacggcg tgtcctgcga atattcaacg accgcttttt caaaggacag ggttccgttt
     7261 ttaatcttat tcgccacatc ggcaagcagg gtcttgttgg cggtgtttac gctgctgtcg
     7321 gaggaatacg gaatgaagat atgggaaagc ttcaccgtct cgggggaaac gaactgtgtt
     7381 ctattctttc ggtaaaacga ggtgacctcg ctttcggcga cgatcggctc ttccttcaac
     7441 acatcggatt tcttcaattg cacgtaggaa tcgacgagca gctgttcgct gatcaattgc
     7501 ttatatgcag caaccgatcc gaagttgttg acgatgattt gatcgaattc ttcattggtc
     7561 aacgtcgttc cgtagctctg ctcgatcgcc gttttttgat ttttataggc ggtttccacc
     7621 atcgtagagg agatcaccac tccgtcgcgc tcggcgcctt ggcgaaagag ttcgttgttg
     7681 atcaaaagat taaggacctg gaggggatcg acggaagagg gatcagaagt tgcagcctga
     7741 tacctggcga cttcagcatc cagatcattc atggaaattt gaacggtttt ggttaagcgg
     7801 accgtagcag cagggctgct gatcgtggcg gcgaagacca gagacatggt cataagaatc
     7861 agaatcgtta aagatgcaaa ccgtttcatc gtgtgaactc ctctctctga taatcaaatc
     7921 taccggtgga aagtaaccgt taaatctgaa tacgcttgcc gatcgctttg gcaagatcgg
     7981 gctttttgat cacttcactt tttttgagga acatcccctc tttctccatt gccttcatca
     8041 gcgtctggag cgtcttcatc gttcgaactc ccccgccggt gataaacgcc atgcagcgct
     8101 tgccgctgag ggttcccgcc cccttgagaa aggtgggaac gacttcgggg atctttcccc
     8161 cgaagaacgt ggtattgttc gttccgatca ccaggtagtc atagaaggaa acgatcttgc
     8221 cgacctccag attcatatcg atgagatcga ctcgatgccc ctggctttga attccttcac
     8281 tcaagccctg agcgatcgcc ttcatcttcc catcgttttt ttgacttgaa ccgtagagaa
     8341 taccaacatt cataccagcc cccttggaaa cagaagccga ccgcatcgag tgcggccgga
     8401 gagatcaaga gcaacactcc gcaatgagtg caacctctta attgcctgca tcccaccatt
     8461 cagtggtctg ttggacttgc tcggtggtga ccgaatcaag gagtcgatct tggcttggcg
     8521 tcttgttgat gaacgcaaca agaagggcaa gcaccaggaa gagcgcagcc aatataccgg
     8581 tggctttcgt cactacgctg cttgtttgtg aaccgaaagc ggtttggctt cctccaccga
     8641 aaataccacc gagaccctcg ctctgctcat cttggattgc aaccaagaag atcaataaaa
     8701 gactcacgat gataaagaga atcaatatga ttacacttaa aacacccatt ggaattcgta
     8761 tctccttact tatcgaagtt cacgattggc agaaattgct ccacggaaag ggaggcaccg
     8821 ccgaccagtg cgccatcgat gtgtggcatg gccatgaggg ctttcgcatt tgaagccttc
     8881 actgaaccac catactgtat tacagttgct tctgcaacac ccttggagaa gagtttttca
     8941 acgacggagc ggacgaatgc atgggcatta ttcgcatcat ccggggttgc ggtcttcccg
     9001 gttccaatcg cccagaccgg ctcataggcg atcgtgacct tccccatctg atcggccttc
     9061 accccgccaa gcccgactcg gacctggcgc tcaagaacct cttccaagcg cccgctttcc
     9121 cgctcttcaa gcgtttctcc gatgcagaac acgacattca tatcgtgctt gagcgcaagc
     9181 agcaattttt cgttgatcac ttcgtcgcat tcgccataga tgcttcggcg ttcgctatgc
     9241 ccgaggatca cgtcgttgac gccgagatcc ttcagcatca aggggctgac ttcaccggtg
     9301 tatgcgccgc tttcatggtt ggccatgttt tgagcggcca cgatcaggct tgaacctttg
     9361 gcggcttcca atactgcggg aattgttaca aacggagggg cgaccatcac ttttgaattg
     9421 gtgtttttaa tcgctttgac aagctccttc gcgaaggcgg cgccttcgct cggggtcatg
     9481 ttcatcttcc aatttccggc gatatatgct tgtttcatgt ttatttctcc aacgctttga
     9541 tgccgggcag ggttttaccc tcgaggaact ccagagaagc tccaccaccg gttgaaacgt
     9601 gactgatctt atcggccaag tcgaatttgt tgatcgccgc aacgctgtcg ccgcctccga
     9661 cgacggtgat cgctttactt tccgccaacg ctttggcgac ggcaagcgta cccttggcaa
     9721 acgtatcaaa ttcaaagacg cccatcggtc cgttccacac gatgctcttt gccccttgca
     9781 gggcatcggt gatcaacttc accgttttgg gaccgatgtc cattccgatc agattttcgg
     9841 ggatatcgac gccgtcgacg acgattgccg gagtcttctc atcaaattca cttccacaga
     9901 ggtggtcgac cggaaggatg actcggacgt tttgcttttc agcctgcttc aggaacgaag
     9961 cggcggtttc cttgtactcc tcttcaacga ggctcttgcc gatcgcatgg ccttggaccg
    10021 ccaggaaggt gtacgccatt cctccgccga tcacgattgc atcgcatgtc ttcaaaaggg
    10081 attcaagaac cgagatttta ctggaaacct tcgaaccgcc gatgatcgcg acaaacggct
    10141 tttccgggtt cgaaagcaac ggagccatga acttgacttc cttttcgatc aggagaccgg
    10201 cataggaagg aaggaagtgg gcaacccctt cggtggacgc gtgggcccgg tgggcggtgc
    10261 cgaatgcatc gttgacgtag agatccccgt atgaagccag ggtttttgca aactcggaat
    10321 cattggcttc ctcttcggca tagaagcgaa cgttctcaag cagaaggaca tcccccttct
    10381 tcaacgcgtt gaccgccgcc tcaacttcag gtccgatgac atcccccgcc aacgtcacct
    10441 tttttcctaa gagctcttca aagcgcttgg cgatcggggc catggagaat tcgggattct
    10501 tctttccctt cggtcggccg aaatggctca tcacgatcag ggaagcaccc ttgtccaaca
    10561 gggcgttgat cgtcggcaga gctgcccgaa ttcgggtgtc atcggtaacg tttccgtcct
    10621 tcacgggaac gttgaagtct actctgatca ggactttttt gtttgtgcaa tcgcacatgg
    10681 taattgtttg taaggtcatt gtcatcctct tttcggtaat tcaggcctgc cgaacggaca
    10741 ggcctgaaga atggtattgt tcagcttatt tcaccagctt ctttacaaga tcgacaacac
    10801 ggtgggagta gcccatctcg ttgtcatacc aggaaagaac cttgacgagc ttgtcaccca
    10861 tcttcatcgt gagttgggcg tcaaaaatcg aagagtggtg gttgccaatg atgtcgcggc
    10921 tgacgatcgg gtcttcggtg tattccaaga cgcccttcat cgaaccttcg gaagcggcct
    10981 tcatcgcggc gttgatctct tcaacacttg catccttttc aagttcgacg accaagtcaa
    11041 cgaccgatcc ggtcggaacg ggaacgcgca tcgccatccc gttgagcttg cccttcaggg
    11101 aaggaatgac ctcgctgact gcacgggcgg ctccggtggt ggtgggaatg atcgaaacgg
    11161 cgccggcacg ggctcttcga agatccttgt gcggctgatc aagcatcact tggtcattgg
    11221 tgtaggcgtg gacggtggtc atcaaaccct tcacaattcc gaagttgtcg tcaagaactt
    11281 tggcgatcgg agcaagacag ttcgtggtgc aggaagcatt gctgaacgct tcatgggaaa
    11341 gatcgagctg atcatcgttg acaccgagga cgatcgtctg atcgatcttg tcttttgcag
    11401 gtacggtaag aacgactttc ttggcaccgg cttttatgtg gtccttgtat ccgcctttcg
    11461 ggctttcggc tacggtgaac acaccggtgg actcgatcac gacatcgaca ccaagatctt
    11521 tccacggcag gtttgccgga ctgcgttcgg cgaagacggg aatcttcttt ccatcaacaa
    11581 cgattgcatc accgtcgacg gcgacactct tgtcatacac accatagttg gagtcatatt
    11641 tcagcaagtg ggcgagcgtc tttggatcag tgaggtcgtt aattccgaca atctcaatat
    11701 ctttgtctac aaatgcaatc ttgaagacgt tgcgtccgat tcttccgaat ccgttaatcg
    11761 caattttcat cgttggttcc tccaggaaat cttattaact attcgatatt aatataccgt
    11821 tttgtcgcat acgtggcaac gtccctacga acgtttgagt atcaatttca cctcgttgat
    11881 cttatgtcca tcgatatcgt caatgatgaa aagcgcatca ttctcttcaa tctcttcccc
    11941 ctttttggga attcgaccaa agagttcgaa cacatatcca ccaatcgttt caaactcttc
    12001 atcactgaga tccagtgagc acacttcatt tacttcatca attgaagtcc gggcatcgat
    12061 gaggtaggta tgctcgtcga tccgacgaat ttcttcgtct tcgtcctcat cgaactcatc
    12121 ctgaatttca ccgacgatca cttcaaggat gtcttccatg cagatgattc cactgacccc
    12181 tccgtactcg tcgattgcga tcgcgatatg gactttccgt tttttgaact ccctcaataa
    12241 atcatccaga tgtttcgaat cggggatgaa atagggtttt ctcatcaatt gttttacatc
    12301 aaaggggttg gggatctccc gacggagcat gtccttggca tagagcacac cgacgatatt
    12361 gtcgatcgtc tgttcataca ccggataccg tgaatacccc tgctcctcga tgatcgaata
    12421 gagatcttca agggacgtcg tactatcaat gaattgcaca tcgacgcgcg ggaccatcac
    12481 ttctttgaca gtcagttcct tcagctcgac aatgccttca atcatctcct tgcgctcttc
    12541 cagctcttgg tagggcaaag gttcattcgc agcttttcga cgaaagagcg atgttaggga
    12601 ccgtttcaat attcactcct caaagatgac cgagtatctt ttcttgcaag aggagcatct
    12661 cttcacgctc ctcattggtt gcatggtcgc gccccaaaag gtgcagaacc ccatggatga
    12721 gcaagcgctg caattcaatg gaaggttcta catcgaatag taatgcattt ctttccatgg
    12781 tatcaagact tataacaata tcgcccaaga tccgtacagg accgaaggac tcccctccct
    12841 cttcctgagc gaaggaaagc acatccgtcg gctcatcaag gttccgatac cggttgttca
    12901 actgttgcat cgcctcatcg gtgacaaagc ttacgcttac ttcacacggt ccgaccctga
    12961 ggtagttcaa cgtgaaaatg agcgtcgctt caacgctctc ggtatcgacg gccatcccgt
    13021 cgtcaacggc gatatcgatc gtgtagggtt ccatcactcc tcctcctgag gatactcgat
    13081 ccgatgatga tcgcgaccga ttatctgctg aaggaacact tcctcgatcc gatccagatc
    13141 ggtcaacgaa agaccggaat ccttcagttg tccccgatcg accttgccca tgatcagctt
    13201 ccgaatctgt ttctgatact ttgaatgggt tggcttcttc atcgtccgcg tagccgcttc
    13261 caccacgtcg gcaagcatca ccaccgccgc ttccttggtc tggggaatct cattgttgta
    13321 ggagtaatcc tcgatgttca cctctcctga gcgctcctgc gcctctttga tcgcttcgga
    13381 gtagaaaaat tgaatgacat cattgccgtg gtgctggccg atgatatcca acacctcttg
    13441 aggaagaccg gcctcacgcc ccttctccac cccgagcttc acgtggcttt tgatgatcgc
    13501 caccgaaaga ctcggcttga tatcatcatg tttattttct tccgtctgat tctcgatgta
    13561 gtactcggga tgatcgcttt ttccgatatc gtggaacatc gctcccactc gcgccaacaa
    13621 ggggttcgcc ccgaccgttt ttgccgccac ataggcaagc tcggcgacgt ggcggctgtg
    13681 ggtgaaggaa ccttgagcga cttgagccat ccggtcgaga agggggttgt cttcccggga
    13741 gatttccatc aatcgatagg gagtgggaat attcagcaat cgttcaaaga tgggaacaga
    13801 tacttcgacc agaatcatcg aaacggtaac gttgatgatc atcccgaaga ccagaggcag
    13861 gtagagatgg gaatcaaggc cgacgagcat gttggctacc agagcggcga agctcgtggt
    13921 gatcgctcca tacgcccaat tggaaagggt gtcaaggcgc cgctgagcgg attgaaacgt
    13981 ccaaagaacc agagaaccgg tggcgatact gaagaagaac gtcatcatcg atgaagtcgg
    14041 cagcagcgcc acatagctgc tgatcatcag ggaggtgatg aatgccagcc gttttttatc
    14101 ggtaatctgc aggatgaaca tcggagccag caaaacggga agatgggagt ccaagaccgt
    14161 tcccggcatc aaccatccga tgagaccgat gagcagaacg gagataagga gaacgctcag
    14221 tgccagtatg agatagagat tgcttcgaaa gtggccctgg gcgaagagga tgaacgcccg
    14281 acatccgaca agcgtcacca taatcgtgaa gatcgctcga gcaagcagct gagtcggagt
    14341 atagaggaag gagtactcgt tcatcgtttc aagcattctc acttggttcg cggtaagggg
    14401 tgtatccttg ctcaccagct tcgtgttttt cggaatggcg atctgaacca attcaacctt
    14461 ggcgcgggct tgagagcgca gactcaatgt cttcgcctcg tcatagatga gattgttttc
    14521 caacagcaat accagcgtat cgatgatcag ctgtggctgg aaagagctct gttggcgaat
    14581 atatcccctg aggtatgcaa agagcagatc atcaagattt tctttggtga ggacttcatc
    14641 caaggagtcg ctttcatccc catgcgattg cacacccatt tcgaggtgga acgaatcata
    14701 tccatcactt gtgattcgtt catagctgtc atgatccatc aagccgcggg agaggaccat
    14761 gcttgccgtc tcgttgaggg tggcgacgta ataggcaagc tccgcttcct tcaacaggaa
    14821 aaggcgctgc aggaggtttc ttcggtcaac cagattttcg ctgcccagga atgtatacag
    14881 attgctttcc cgctgatccg tttcacttaa cagaagtttt gagaaaatat ccaagcgggt
    14941 ataggaaagc gaagtttgtc ggaggttcag cgaagcgatc ggaagaatcg cccgctcggc
    15001 ggcaagtcgt cgttcttcgg ttttttccgc atcgacgtgg atgaaatccc gcggagcgaa
    15061 gatgtcttct tccgccaact gccccacttg gtaggtgaat tgcttcgaag cggaagagat
    15121 atgaggaagc agcgggatga agaacgcgca cccggtaacc aggatcatca acataatgat
    15181 gatcaatggt tgataccggc tgccggctga acgctgacga ctcaattttc gcatttacgc
    15241 atctccattc tgatatgcgt caataatcgc ctggacgatt cttgagcgca ccacatcgct
    15301 tgaagtgaag tgggcaaacg ccagctcgtc gattccctga agaatcttct ccgcatggac
    15361 cagtccgctc atctttgact tgggcagatc gatctgactg atatcaccgg tgacgatcgc
    15421 cttcgagtcc tcccccaacc gggtgaggaa catatgcatc tgttcaacgg tggtgttctg
    15481 cgcttcatca agaatgacgg tggcaccccg aagggaacgg ccgcgcatgt acgcaagagg
    15541 agcgatctcg atatttcctc catcctccag cttccgaatg tttgccggag agatgagcag
    15601 ctccatcgca tcatacaaag gccgcaggta gggattgatt ttctgggcca aatcgccggg
    15661 aagaaacccc agactctccc ccgcctcgac gatcggccgg gtgaggatga tcttgcgctt
    15721 ccggccgctg aaaagctggc tgagggcgta ggcgaccgca aggaaggtct tccccgtccc
    15781 tgccggcccg atagcgaaga cgatcttgtt ctcctccatc agctcgacat aacgctcctg
    15841 gtttgctcct ttggcgtagg ctcggcgatt gtgaatcttg attgtttttc ggttctcccg
    15901 atccccgttg accacatccc tgtcctggct cacatcattc ttgattgatt ggaactccat
    15961 gaagatatcc gtttcattca ggaagagctg atgctcggcg agccggctga gccggccgaa
    16021 gagcaggagg aagcggtttt gaatgagcgg atcgtcactg agacagatga catcgttcga
    16081 gcggacgaac aactcgcacc ccaacaacgt ttccatgtat gggatgtttc gatcgttgat
    16141 gccgaggact cggcgaacta attcttcact gtcaaaggtg attcgttttt ccatacccat
    16201 cctatcgcat tacagggaac gatcccaaac accaccggtt cgcgaccatt tctcttcaat
    16261 atcaagatcg ggaatcgtat tccatgccag gtagatgctg aaggtcggca cccaccgata
    16321 ttgattgttc gatagtacaa cacttccact gtatttacaa ttcagtctcc aatcctccag
    16381 atagtgaatc agctggatat ccacagagga gaggttgaat tgggtatgat ggattccccc
    16441 tccgaagaga tcgaaactgc gcagaagatc cttccaaaga agaagccagg caaaggagcc
    16501 cgcatcgtcc tgatagtgat ggaagccggt gttggaggag gcaagagcga tcgacagatc
    16561 gaggaattcg gcgatttgga agcgactctt caaggagaac gtgaagaccg aggagaaggg
    16621 atcatcaaca tcgtaatcaa acgctgcgtt caccccaagg gtcagagcga tccgtttctt
    16681 atagaagcgc cactgccttt cagcgagact caccatagcg gtgagccgct cgctctgtat
    16741 gttgccaagg gttccgcccg catggtagtt caatgtgaga aaggaggttt tcagccccag
    16801 cactccatgg gaaagaacgt gttcgactcc gtttttcatt gggaagtact cgaatcgttc
    16861 actgaggacc agttctttgt caaagagcga aagggtgagc tgatgatcca gacgggaggg
    16921 ttccaacggt ttgaggtagt tataggagaa ggtggtgttg gtcacgaggg gggaatcgct
    16981 atatccgact ccaaggctga aaagctcctt ctccagaaat ccggttccgc ttgcatcttt
    17041 gaatcgcagc gatgtcgtca aagatgctcc ttgatagcgc acaatcactt tgggagcaag
    17101 ggtccgggtg agcggccaca gaacggcggt gatcgaagga ttgatcgtac ccgcccataa
    17161 gcgataggtc cgatcgaacc gcagttgatg agtgctcacc gatttctcat caaacctgaa
    17221 gcgatcgatg accacattct tcggtttgtc gccgctttga gtctcttcct ggcgatacag
    17281 ccgttggcga agggtgtagg atacaccgat gaacggcagg tgaagcgttg tgtttgcatg
    17341 cagttggaag acttgggtgt ggtttgtggt tttttgcggg tcatcgctga ctgagagctg
    17401 tggcagcagc tcaaaggagg aggtgaagaa gcggctgtcg ggaaccgctg aaagaatcaa
    17461 ggttcctctc gtcatcgaat acacgttggg gtcatccccc gccgtcttgt tgtacgttcg
    17521 agcgaactcc tcgctgatcg tataggagag cttcaatgag cgctcggctt gacgtgaaaa
    17581 gagcgaggcg ggctgctgcc gttgggggcg gtagagattc ggcagcaaag gattgacatc
    17641 cggagtattc gaaaccggag ggctttttac cgtggtctgc ccggcaaggt tcaaaagtgt
    17701 tccgctcgct cggatgatga accgcggcag ggtcacttca gtgaggtgat atgcatggat
    17761 tccatctttt ttttgccaac gatatgttcc ctgaagagcg atattgtcga tcgtgagcga
    17821 agaaagatac tctttgaaga tcgttgtggg gatcgtgagg tttccggtca aacccgtcgt
    17881 gtaggaggag cgctccgaac cgagggaggg aaactcctgt accgaaccga agagcgcatc
    17941 caaccgaaga ttgtgcaagc ggcttgaata gagccgctcg acgctcggat cgctgtagta
    18001 tggcagggtt gccgtaagag aggaggaacc caccctgtag aagagcgtgt tcatgctcca
    18061 catgcgcact ttgttgacat ccgaccaggc aacgcgttga tcgatcccgg atggatcaag
    18121 ggcgacggtg gaaagcgaat tgatcgtgag agtgttcttc agcagcttga tccgggaatc
    18181 atatccgagg ctcaccccgg tatcttcgta tgcatcaccg aaaatcgcca gatatgagga
    18241 ggattccctt gcccattgct cgaatgaaga tggagctttc tccagtgtgt atatcggcca
    18301 tgcacggatg gctcctgctt cctctttcgc tgacaacagg gttgccaacg accgttgttc
    18361 atcctttcgg aaatggggat atcttccgaa gatttcccac gtggtattta cgaacatccc
    18421 tcgattgctg atgaacccca tcgccggatt ggcgatcatc cgtgaaccgg gaatgaagag
    18481 aaagggtgtc caaaagagcg gaacccggcc gattgaaagg gtcgccccat gggcgatgat
    18541 atccccgtcg ttcagcacgc tgatccgctt tgcccgaatc gatgaaagcg gatcgatcgc
    18601 tcgggtgccg atccgggcgt cggtgtaggt gatcatcgaa ctcgcgcttt gaatcgtgat
    18661 cctcgttcca agggtgtgga gggtgacagg ctgatcaccg agctcttcgc tgattgtaac
    18721 cagatcggcg caggaggcga agatatctcc ccgttcgtac atcagggtga tgatctctcc
    18781 actgatttcc tttgcgccct cggtacggac cgaacccatc gctgtcagac ttttatcgga
    18841 tacatcaaca aggagccggt cgctgtagag acggattttc tcctcgctgc cctcttcttc
    18901 gatcatcaga tgaacgtttc ctttgaggag atatcgtccc ccttccaaac tctcaaatcc
    18961 gtcggcgttg atgatgttga ggatgtattc ctgcccactc tcacgaccga acaatgaacc
    19021 gtttacgcac agtgcgagca ggaacacgag gaaacagaaa gatgatgcga ccctagcctt
    19081 tttcatggaa catctgagca agataatacc ccgtgtaaga gtccttgcat agggcgagct
    19141 gctccggtgt tcccgaggct acgaccattc ctcctccatc cccgccttcg gggccaagat
    19201 cgatcagatg atccgcttga gcgatcacat ccaggttgtg ttcgatcagg aggacggtgt
    19261 ttccctggtc gaccagccgg ttcagaacct cgagcagctt cttcacatcg gcaaaatgca
    19321 ggccggtcgt cggttcgtca agcacataga gggttttacc ggtggagagc ttggaaagct
    19381 ccagggaaag cttcacccgc tgcgcctccc ctcccgatag ggtgagggcg ctctgcccga
    19441 gtttgatata ttccagaccg acgctgatga ccgtatcgat cttccgtttg atcttcggaa
    19501 tcgcattgaa gaactcggaa gcctccccca tcgacatctc cagcacgtca tggatgttct
    19561 tgcccttgta gcggaccgca agcgtttcct ggttgaagcg cttgccgtgg catacatcgc
    19621 aggtgacata cacatcgggc aggaagttca tttcgatctt caacgttccg tcccctttgc
    19681 agttctcgca gcgcccgccg gggacgttga aggaaaagcg gccgctcttg tacccccgag
    19741 cccgggattc cggcagcgat gcgaagagct cgcggatcgg cgtaaacagc cccacgtagg
    19801 tcgcagggtt gcttcggggg gttcggccga tcggggtctg gtcgatgttg atcaccttgt
    19861 cgatatgctc gaccccgctg atcgaagagt atcctttgta gttcggctgc ttcttcgcca
    19921 gcgtccttcg aacagcaggc agaaggactt cgttgagcag cgaggatttg ccgctgcccg
    19981 aaaccccggt gaacaccacc agcttcccca acgggatatc gacatcgatg tgcttcaggt
    20041 tgttcttgtt cgctcctccg atgtgaagga tctctccgtt cccgcttctt cgttccttgg
    20101 gaatttcaat caccagggaa ccgctgagat attggccggt gatcgaggcg gggttcgctt
    20161 cgatctcttg gggcgttccc tgggcgatga ttcttccgcc atggattccg gcgccgggac
    20221 cgaggtctac tacccaatcc gcctcgcgga tcgtcgcttc gtcatgctcg accaccagca
    20281 cggtgttgcc cagatccctg aggcgcttca gggtatcgat gagcttctga ttatcccgct
    20341 gatgaagacc gatcgagggt tcatccagaa cgtacaggac tccgctgagc gccgatccga
    20401 tttgcgtggc caggcggatc cgctgggcct cccctccgct caaggtggcg cttgaacgat
    20461 caagggtgag ataggtcagc cccacatccc gcagaaagag caggcgcgaa cgaatctcct
    20521 tcaacacttg ggcggcaatg accgtctggc tctcattgag ggcgatagtg tcaaaaaact
    20581 ccttggattg ggcgacgctt tggcgagtcg cctccatgat ggagagccct tgaaccgtta
    20641 ccgccaacgc ttccggccgc aggcgttctc cgctacaggc ggggcatacc ttggtcgtct
    20701 gaaaaccgtt catccaggtg cggatatgca tgctgggcgt ttcatagtac cggcgctgaa
    20761 gatcgggaat cacccccgga tacggcttgg cgacatggat cgtccgattg cctttatccc
    20821 gtacgtattc gaccatcacc tgctcctccg ttccccagag gatcgcctga agctgctctt
    20881 cattcagctc gctgaacggg gtatccaagg tgatgccgat gtgttttgcc aaagcggaaa
    20941 actggctgcg gctccattgg gcatcggggt tcatcgtggc gatcgcccct tcgttgaagc
    21001 tcttgctgta atcggggatg atcaggtcgg gatcgaactc cgttttgaac cccaggccgt
    21061 tgcactcggg gcaggctccg aacggattgt tgaaggaaaa gaggcgcggc tcaagctccg
    21121 ggagcgtgat gccgcagtgg gggcagctgt tccgttcgct gtagatttcc tcgtgggagg
    21181 aacccgcttc accgaggaag gtgatcttca ccagcccctg cgtcatctcg atcgccgttt
    21241 cgatcgaaga ggccaagcgg gtccgaatgt ccgctgagag gatcaggcgg tcgacgatga
    21301 tgtcgatcga atgcttgatc tgcttgtcga gggcgggaat gccgtcttca agatcgatct
    21361 gctgtccgtc gacgacgagg cgatgatacc cttgggcctt cgcatcctcg aagactttcc
    21421 tgaactcccc tttcttcccg atcgccacgg ggctgctgac gatgatccgg ctttgatctt
    21481 tcgccttgaa gattgcatcg atgatctggt cgacgctcat ctcgctgatc tttcggtgac
    21541 acacatggca gtgcggggtt ccgacccggg cccaaagaag gcggaagtag tcgtagatct
    21601 ccgtcaccgt gccgacggtc gaacggggat tgcgctgggt cgagcgctgt tcgatcgcga
    21661 tcgccgggga gaggccttcc atgtagtcga catccggctt gtccaacttg tcgaggaaca
    21721 tccgggcata ggaggagagc gattcgacgt accgcctctg cccttcggca aagatcgtgt
    21781 cgaacgccag ggaggatttc ccgcttccgg agattccgct gatgatgatc agctgatttt
    21841 tcgtaagggt caggtcgata ttctttaagt tgtgttcccg agcacctttg atgaccagct
    21901 tattctgcat ttcgaatcgc ttccagaagc gcctctttcg cgtcttgggc cgtcaccttc
    21961 cggctgagca cgcctttttc atagagaaag atcgcattgc cgattccggt gatcgccaca
    22021 tcggcctcct tcgcctcccc cggaccgttc acctgacaac ccatgattgc cacggtcaga
    22081 tccttttcga tcgcaagcag ctcctcctcc accgatgaaa ggaagcctat cgtatcgaag
    22141 gtgtggcggc cgcagcgggg acaggaaacg actctgattc ccttcttttt cagaccgagc
    22201 gtcctcaaaa gctcaacacc cgcctgaatc tccgtttcga tttctccggt gatggagatc
    22261 ctgagcgtgt ttccgatccc ctcgctgagc agggttccga gcgcccaggt cgaacgggtt
    22321 accgccgaga tgaccgatcc ggcttcggta accccgaggt ggatcggata ggagctttct
    22381 tttgcaaaaa gccgattggc ctcgatcgtc aaatcggtat cgcttccctt cagggaaacc
    22441 accgtgttgg tgaagccgag acgctcgaac caatccagat attccaaggc ggtatccttc
    22501 atcagatggg ccggtgaagc cgcttccctg cgcggcaggg aaccgctgtt caatccgatc
    22561 cggattgcga tgtcgcggtc cttcgccgcc cggaccacct cgtcgacttt ccacctcgct
    22621 ccgatgtttc ccgggttgat ccggatcttg tgcgctccgg cttcgatcgc atccaacgcc
    22681 agagtatgat cgaagtggat atccgcgacg atcgggatcg gggaggcttt cgcaaggtat
    22741 ttgaacacct catgatcatc cttcgagggg tagctgaaac ggatgatgtc gcagccgatc
    22801 gtattcagtt tgccgattcg aatgagcagc gcctcggccc tcttcatatc aaccggcaac
    22861 gcatcatcat acatcgtttg gacgaggatt ggcttgccct tcccgatcgt cagatttcct
    22921 atatggacaa gatgaacgtc attcatcgtt ttttctcctt gagttgagtg tactaaagga
    22981 ttggcgtttt gaatagtgat actacacgct tgtgtagctt atcggacttc gacaagcagt
    23041 cgggcgacgg cggcctcttt ttccacgatg agggcgtagg gctcataggg gtgaaagaag
    23101 atgaagtgat tcgcatccac ccgagccgtc gaggtgatcc cgctcgattc atggtgtacg
    23161 aacccgttga tgtctctaag gagggtgccc ggctgccggg catgggtcga acaggcgaga
    23221 acctctcctt ccttgacaag aaacaccgtg ccccagtgac tgtgcgcggt gaacatcccg
    23281 tcgaagggct ccggatggtg cggttcatac accggcttgt ccactatgaa gagatgggcg
    23341 taatggtcaa gcgaatcaag cgtatcgtag atcatggttg taccgtaaag ccccggcggt
    23401 tccattgcaa gaggatcgcc ggtcaattat cctatcatca tgaagatgac ggccctctat
    23461 caaagcccgc tcggcccgtt gtatttcatt gaagaagatg aacgcctcgt tgaagtcgga
    23521 ttcggcgaac gggcggaaac atccaccacc ccattgctca gggaagcgat gaggcagatg
    23581 gatgcatatt tcaatgacac cctcacgagg ttcgacctgc cgctccaccc gagaggcacc
    23641 gagtttcagc tgaaggtgtg ggaagccctt caaacgattc cctacggcca aacgcgttcc
    23701 taccgggata tcgcccgaat cgtcggaaat gtcaaagcgg tccgggccgt cggcggggcg
    23761 aacaacaaaa acccgatttc catcatcatc ccctgtcacc gggtgatcgg gaaaagcggc
    23821 aaattgatcg gttacggcgg cggcttggag aaaaaagcat ggctgctggc atttgaaaag
    23881 cggacaccgt cgtgatgtcc gctttcctac ccgttgttcg gctaaaaaag attattgcaa
    23941 cgcaagcaag ctgaagacaa gggtaaagag agcaaccgtt tcaacgatac cgatgacgat
    24001 gaagtagttt gcggttccct ttccggtttc ggcaagagca tcacatgcgg ctgcagcagc
    24061 ctttccctgc atataggcgg aaagaccgat cgccaacccg ccgagaacac cgacaaacaa
    24121 agccaatgtc gaagcgatgc cgccggcaat cgcatttgcg atgaagttca tcaagaggaa
    24181 gccgtaaatc gtctgagtga gcggagctcc ggcaaacgcg accatgatga agggagcggg
    24241 cttattctcg acataacact tcttccagcc gccgaccgca gccatggagg cgattccggc
    24301 gccgattccc gatccgagag cagaaagggt caaagccgct gccattccga taaattccat
    24361 attaaccatc tcgttctcct tgtgttgttc caaacaacgg tgaggtattt attcttcgac
    24421 cgtttccgcg aacggttgat attgaatccc ggtccactcc atcccgagtt gtccggaaaa
    24481 ctccaacaga ttgagccgaa cgccgtgaac cacgacgctg agcagaccca tgaccagatt
    24541 gagtccatga ccgatgacca ataccaggat tcccgccgga agcgcaaatc ccttgagcat
    24601 tccgcttgcc atcgcattaa aggattgggc gatcgccacc gatgccattc cgacggcaaa
    24661 gaggcgaatg tagctcataa tattgctgaa ggcgctgatc gtgtccagga acgtggtgaa
    24721 gaaaccggcc aagcccccct ttatcccatc gataaacgat actcccggtc cttgcgctcc
    24781 gaagatcagg atcgtcacga aagcgactcc gaccgtggca aagagcgcct tgatattcac
    24841 ttgcgctccg atgaccagca gcaggacgat gtaatagagc gccagaagag cgatgagcca
    24901 actgagatcc gctaccagac tgagatcctt aatcctggcc ttgcgaacca cattgatgat
    24961 acacgccaaa gaaagctgga cggttccaat gatgaaagtg aacttcatca cggtgttctg
    25021 ggcggtgacc gcctcgatgc cgaacagctc ggggtagttg gagatctggg ggatcaccag
    25081 cttctgcagg aacggaatgc tttgaaggat ttcaatcgat ccgaaccacg ttccggtgag
    25141 cgagccccag acgatcgtgg cgatcgagag cacatagacg agcatcacca gcgtattcgc
    25201 tttcttcatg gagatatgga tcgcacccgc aatgagcagg aagatcaatc cgtagccggc
    25261 atctccgatg atcatcgcga agaacagggt gaagaacaga aggaaccacg ttgaaatgtc
    25321 atgttcccgg tatcccggca ccgttccgag gatatcgaac accggcttga tgatgccgac
    25381 acccttcctg tactcgatca gggtcggcac atcttcatca tcctcgctga catcctcgag
    25441 gagatacgcc cacccgttct tcttggccaa gcgggtgaac tgctcttctt ccttcgtcgg
    25501 cagatatccg accagatagg aaagttcttc atcgtcgaca agggacgctt cgacctcttc
    25561 aaagcgcgtt ctcatctcca gaacactccg gtagtgcttc agcgcatcaa gatggacaag
    25621 gcttgctttc agtttctcat cgattcggac aagctccttg ccggcttgat cgatcgatcg
    25681 ctgaagatcg gaaaggcgac ctttggcgaa cgttgccggc accgcaccgg tcgacggatc
    25741 gagctgctcg ccgatggtag cgatcgcttt catcggtcct cgataggaca cttcatagta
    25801 gacctgttcc tcatcaagct tggccagatc cttcttgtcg atcgtataga agaagagttc
    25861 gatcccttcc cttttcagcc aggcgagctc ttcgggatcg aaatcgcccc atggcagcag
    25921 ggtggcggcg gaaagtgaat cacgatcacg cttttggatc aactcgtttt gtttgttgag
    25981 cagtgatatc agataggcgg ttgtttccac aacctctctt ctgctcaagg ttttttgtgc
    26041 tgccggctgc ttcttgtcaa ccagctcctg cagcaccgaa atgaccttct cgacatcttc
    26101 gatctcttgt ttgtacgaac catcgaaagc tttcgaggcg gtgctgacgt gcaacactcc
    26161 cgctttgcga agattcttca gcatcgcttt cgttttggag cgctgcacca cgatataggt
    26221 ttttttcatc tctgctatca tgtcagactc ctgcgaccaa tttcttcttg gcgatcttcc
    26281 ctcgaaccac cgctgcggtc tgctggtcgc ccagatagat gccgatcagt cgaatgtgtt
    26341 ccttcgcttc gggaatcttc accttttcaa agaggtttac tctttgactg gttatcctca
    26401 gttccctgcc caacagctcg atctgctcgt tgagtgtggc aatcagggca tcatattggg
    26461 cgacctcccg aaggaagctt actcccttgt cgatccaaag cggatagtga agcgggttgt
    26521 attcaatgtg cttgaacgtc aactccttga aggtgggaat gatgaccccg gcgatattgc
    26581 tcctgctctt gagaatcgtg tcgatttgta caagcgaaag aagatcatac cgctcatttt
    26641 cggcgtacac ggcgatccag gtttttacct gggcaatgag cgcggcctgc ttttgcttat
    26701 gctcttcgat ctcacgctcg atctgttgaa tcaccagctg aagctgctgc ttcttgagca
    26761 tcagcgtggg cagatagcgc tcaaactgct tgaggcggtc tttttggagt ttcagttcat
    26821 tctttgtcag tttgacagcc atgaatgctc cttactcacc gagcggtttc ggccagcggg
    26881 ctcgaatcaa atcggaccgc agtccggttt cgttgggctc gaaacaatcg ccgagaatct
    26941 cccagcccag atccannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagtcc
    27061 ggtttcgttg ggctcgaaac aatcgccgag aatctcccag cccagatcca gcgcctcttc
    27121 aagggggatg ttcactgaaa gatccatcat cttctcttca aagagatctc cgtatttcag
    27181 cagtttttca tcccactcgc tcatcatgaa gcccatcgac tgcttttcga gggcttcctt
    27241 gaaggaggcg tagagccgga tcataccgtc catcaatgcc cggtggtcat cccgggtggc
    27301 gctgttgacc tgctgcttca gacgggaaag cgagccgaac ggttcgattc ttccgccctt
    27361 gagatagtat tgcccttcag tgatataacc ggtgttatcg ggaatcgggt gggtcacgtc
    27421 atcaccgggc atcgtggtga cggcaaggat cgtaacggaa cccgctcctt cgaagtcgac
    27481 cgctttctca tagcgggagg cgagctgggt gtacaggtct ccgggatatc cccggttgga
    27541 aggaacctga tccatcgtga tcgccatctc cttcatcgca tcggcaaagt tcgtcatatc
    27601 cgttaacagg acaagaacct tctttccttc caaggcgaac ttctcggcga cggcgagggc
    27661 catgtcggga accagcatgc attcgacggt cgggtcgctg gcggtatgga cgaacatgat
    27721 cgtccgatcc atcgccccgc cctcttccag agtatccttg aagtagaggt agtcgtcgaa
    27781 cttcagcccc atgccgccga ggatgatcag atcgacctcc gcctgcagag cgattcgcgc
    27841 aagcagttcg ttgtacggct cgccgctgac ggagaagatc ggaagcttct ggctttcgac
    27901 aagggtgttg aacaggtcga tcatcggaat accggttcga atcatgtttc ggggaatgat
    27961 ccgcttcgcc gggtttaccg agggaccgcc gatatcgatc atctcttctt tcaattcggg
    28021 cttgccgtcc cggggagagc cggtaccgtc gaacacccgt cccaggaggt tgggcgatga
    28081 ggagacccgc atcggattgc ccagaaagcg gacgtggtcg ccggtcgatg tcccggtggc
    28141 tccgctgaaa atctgtaatg agacaagatc cccgtccagc ttgatcacgt tcgcgaacga
    28201 ggtcgatccg ctgctcgaaa cgacggcaag ctcgccgttt gcaactcctt cggctcggac
    28261 cgtgatgacg tttccaacaa ttgactcaat cttcgaatat attttttgca tgctcttctc
    28321 ccttacccaa gagccttgaa tgcttcatca agggcggctt ccgccgcttt gaactcgtct
    28381 gatccccacc tcgtgttgtt ccagtccagg agaagctgcc ggaggttgtt gaagactcgg
    28441 cggacatcgc tcttcgtctc aaaggtcaac ggcgtgttca tcagccgatg gagctttgca
    28501 aagacgtgct tctggcgctc cggcggcacc gatgtgtcga tcggatcgaa ggagttctgc
    28561 tgaagataac agctgtcgag caactcgccc atctggtagg tcacgtagtc attgatgctt
    28621 gttccctctt caccgacgac cttcatcatt tgattgactt catgagaacc atgcagaacc
    28681 cgccgcatct gattcacctc ggattgcttg atgattcccg gatacttcga ccaagatgtc
    28741 aaagggtcta tagccggata cttccgggca tcggagcgtt cgcggctgag gccgtggaag
    28801 gctccgacga ctttcagcgt cgcttgagtt accggctctt cgaagtttcc gccggcggga
    28861 ctgaccgttc cgccgatcgt aacggagccg gtccttccat cgggcagctt gacgattccg
    28921 gctcgttcat agaattcggc gatccggctt tccaggtaag ccgggaacgc ctcttcaccg
    28981 ggaatctcct ccaagcggcc gctcatctcc cgcatcgcct gagcccagcg gctggtggag
    29041 tcggcaagca gcaggatgtc aagacccatc tgtcgatagt attcggcgat cgttacggct
    29101 gtgtacacgg acgcttcccg actggcgacc ggcatcgagg aggtgttgca gatgatgatc
    29161 gtccgttcca tcagcgaacg gccggttttc gggtcgatca attcggggaa ctccttcaga
    29221 atctcgacga cctcaccggc ccgctcgcca caggcggcga tgatgacgat gtcgacttcg
    29281 gcgttccggc tcgtcatatg ctgaagaacg gtttttccgg caccgaacgg tccgggagtg
    29341 cagaacgttc cgcccttggc gaccggaagc agagtgtcga tgattctgat cttcgtcatc
    29401 atcggctcga cgggcctcag tcgttcgctg taaagccgaa tcgctttctt caccggccag
    29461 ctgaagacca tggacagttc gagcttcttt tttttggagt cctcgacggt gcagatgata
    29521 tcgcgggttt tgtacgtccc cgcttgtttg attgaggtta cggtgaacga gccgacaaga
    29581 tcaaacggaa ccatgatttg gtgggtgaac aatccttccg gcacggttcc gatcgtatcc
    29641 cccgccttga cgatgtcacc tttggatacg gtcggggtga actcccaatc aacgttggga
    29701 agagcctgaa ggtatacacc ccgttgaagg aagaagccgc attgccgggc gagttccggc
    29761 aaggggttct gcagtccgtc atagatctgc gtcaagagcc cgggccccag ctcgacgctg
    29821 agcatctcgc cggtaaactc aacctcgtcg cctacaacga ttccaccggt catctcgaag
    29881 atctgcagag cggcgatgtt gccgttgatc cggatcacct cgcctttgag gcgaatatcc
    29941 ccaagatgga tgtatcccac ttcgtttttg gcgatcgctc cgtcaaactc gacattcacc
    30001 atgttgccgt tcacgccgag cacacgtcct gtgttccttg ccatatccta ctccactaca
    30061 tctggtctat ttccgatctc aatgtgccaa ggagggtttc aaaggtcgcc tggccgacct
    30121 cttgggtgaa gatgttcacc cgttccaaca aaagcaattt aagcccatat cccaagaggg
    30181 catcgaactc gaagaccttc attccgacca accggtcgat ccgtttccag agcagcgtga
    30241 tgaactgcat ctccgcttcc agcggatcgg aacgttcaag cagctcatcg gttacggttt
    30301 caatctcttt ggggaacgtt tgacgatgaa gatcttcatc gatggcaaaa ccgagccgca
    30361 cttgccgttt cttggcgagg gccgtattca aagcggaggc ataacttcgg tatgatgtca
    30421 gcagcgcatg cttgcttggc gttccgctca acccgctttt cagagttttc caatcacgtg
    30481 agttaaggtg tttttccgct tgcgctttga acagctcaac ggtgatgggt gacttttgat
    30541 gaagccggag caacggcagc gttgttgcaa aataataata tgatgccact gccggcccct
    30601 aaaagattcg ctcgtgcaac ggtcgagcaa ggtacggttg caagaggtca gccagttcgc
    30661 ttgccttgta gtcatagaag tacgagccgt cttttgcaac gaggcggaac cccgattcca
    30721 cgtcatcatg gggtttcaga accagaccgc tcttcaattt ggctgccaac gccgtttgaa
    30781 gctcatcggc aagggcttca aaagccttag ggtcgatttc gatgttcaca tcaccgtcac
    30841 cggcctcaag aactttcgtc aacaacccga tgaagtcctt gctgctcagc gccttcgatg
    30901 ttttttcaac aaggatgcgg gaaaactgac cttcgattga ggctttcaag gaaagaagaa
    30961 gatcgcgccc ggcctgagaa agcgcttttt cagcccgccg gttcgccaga gcaatggcct
    31021 cttccgcttc agacactata tgagtcgctt tttcctgcgc ttgagcaatg attgtttgtg
    31081 cttgttcttc cgctgattga agaatggaag ccgcttcctt ttttgccgct tcaaccccgt
    31141 cttttcggat cgaagcaacc aaatcttgaa tctgttgggc catgtgcatc ctaccttctc
    31201 tgatacgttg gtgaaacttc aatcattatt gctgaatagg ggtaaaagct caagagccct
    31261 gaagtgaaac cccaagagat tccccttacc gggtggaggc tgccatgtac taccacgccg
    31321 tcaagaaatc atccgaggtg ctctatcgca caaaggagga agcccagagg ctcctgttcg
    31381 ccctccatgc aaagctgacg aaacagcatg caacgatcct cgactacctg ctggaacccc
    31441 aaacgtgtca acttctgctg caatcaaaga aaccgatcat ccttccggcg tttacgatca
    31501 atccggttga aaaagagaag ctcctgtggt acttctcttc cctcggatcg aaaggaaaga
    31561 cctatccgta cagcgggctg catgagtgct atttcctttc gacgtgcttt tgcgagctgg
    31621 gcaaggtaac agccgatcct ccgccctatc cgctgaagga ggtgctcgcc gtcaagcacg
    31681 gtcgcgcaga atgagggttc gaatcccttc caccgagatt ttgatcactt cgctggtgtc
    31741 gtggacgatc gggttgggaa ggcccgcttt ctcccgctcc tggtttccga cgacgaagaa
    31801 gagcgaccca acccggacgc cgaggtaggc ggcgacggtg aacaaggcgg cgctttccat
    31861 ctcgctggcc aggactccca ggcgcttgta cgcctcccat ttgttctcca gctcgtagaa
    31921 gaccggcatc ttggatggat cgtgctgacc gtaaaaggag tctttgcact gcactacgcc
    31981 ggtatgggga tgtttcccca gtgcgatcgc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32101 nnnnnnnnnn nngagtcttt gcactgcact acgccggtat ggggatgttt ccccagtgcg
    32161 atcgccgcat ccctcaaggc ttcgacgacc tcgaaggtcg ccacggcggg aaactcgatc
    32221 ggggcgtact cgcggctggt tccctccgcc cggacgctgg cggttgcgat gaccacgtcg
    32281 ccgccgatca ccttcgggtc tattccgccg cacgtgccca tccgaacaaa ggtgtccgcc
    32341 ccggatttgt acagttcctc catagcgatc gccgccgaag gtccgccgat tcccgtggag
    32401 gtgacggaga ccttctcccc ctccagatac ccggtgtacg tcacgtactc gcggacgtcc
    32461 gccaccggtc gggcatcggt cagatgcttg gcgatgaggg gcacccgttt gggatcgccc
    32521 ggcatgatga cgtagcgccc gacatccccc ttctttgttc cgaggtgata ctgaattccg
    32581 cttccatggg tatagtcact catatcgact ccttatctct cgattctaca ggaatacata
    32641 cgtttttccc atccctccat cctcgggaag agcaaatcgg taatcgacga cggaacggtg
    32701 gtttttaaga agcctgccga tcccttgact gagaatcccg tcgccgtagc cgtggatgat
    32761 tccgaacgtc gaaaggttgt tcaacagggc cgcttcgata ttctgctcta ccgcttccaa
    32821 ggcttcttcc agcgtgaagc cgcgcacatc gatctcctgt tttgcccgtc tcgcattggc
    32881 ggtgtaggaa acttttaccg tcgaatccct gtttctcgga aggtacaggt cgctctcctt
    32941 caggctcatc cgcatcgaac cgattgcgac aagatagcga ttcctcccct ccttcttgat
    33001 gatcgtgcct tcccgctttg aaggtccgca gaggacctcc atgccgacct cgaaggtcgt
    33061 cggatggaca aacgcctcct ccgttttttc cagcgccgct tcttccgttt caacccgtcg
    33121 gtccagctcg gcgagatagg tctttaccgc ttttgttttt tcaccggtca attctccttc
    33181 acggatatcg gtaaccagct gttccaatct ctgtcgagct ttttgagtga accgggcgag
    33241 ggtccggatt tcctcgtctt tcagctggcg ctcctgttgc ttgatcgtca atgctttcag
    33301 gtcgagcttt cggtactcct ccttccacct gctttcgatc ttcgacaatg ctttgctccg
    33361 ctcctcgacg cgcttgcgct cttcctccaa gcgcatgaga atcgaggaaa gttcgattcg
    33421 gtcgctgccg agcagctccc gagcttctga aagaacttct tccggcaacc ccatcgcttg
    33481 agccgtaacc aacgcatggg attggccgat cacattggga acgatcctga aggtcggctt
    33541 tccgctgact tcatcgaact ccatcgcggc gttgatgatg ttctcttttg aataggcgta
    33601 ttccttcagc acgccgtggt ggctggttac cagggtgagg gcgcttttct tcagtgaata
    33661 ggagagaatc gcctgagcga gggcggatcc ctccagggga tcggttcccg aaccgagctc
    33721 atcgaggatg accagcgagt tcggggtgag gtgactgaga atctcggcaa gacggctcat
    33781 gtggccgctg aacgtcgaaa gctctttttc aatcgattgc tcatcaccga tatcggtaaa
    33841 cacccggtcg aacagcggaa gggcgcttcc ttcccggacg ggaagatagc cgcagtattg
    33901 gttcagcagg gcaaagagtc cgaaggtctt caccgttacg gtcttccccc cggcgttcgg
    33961 tcccgagagg acgactccgt tgatcccctt ctccaggatg atcgtgatcg gaatcgcttt
    34021 ttgtttcaaa agaggatgac gggcgtcgag aagtcggact tcatcgctgt caagccggac
    34081 gatcgtgcat ctctgttcat gggcccagcg ggagagcgtg tagtatccgt cagcggtacc
    34141 gacggtatct ctgagctcgt gcagcagttt ttcttcttgt ttggccttga gagcaagttc
    34201 ttgaaggatt tttgcgattt caaccagaat ccggttgttc gccaggagga cggagttgtt
    34261 caggtcgaca agggcgaacg gctccatgaa caccgtctgg ccgcttgatg aaacggagga
    34321 gatataccct ttgacgttgt gcttgtagcc gtctttgatc ggaatcacca gccgcccgtc
    34381 cctcagcgct tccacgtcgc tttgcacaga ggagcggtta tcgcggatga aggaggaaca
    34441 gtaggaggcg cgcttctgtc ggacctgctc gagctgccgg tacaagggct gcaggcgggg
    34501 atggttctcc cgaaccgaac cgctttcatc cagatcggca aggatcgcct ctttgagggc
    34561 gatgagggcg ggatcgaccc ccaaaccgat cagttccctc agagcttctt cttcatctct
    34621 aagaagataa tcaaacagct cttcggagga ttgaagatag cgggcaagat ccagaagacc
    34681 ggggccatcc ggcgagggag tcggttcacc gaggcgatcc atcacatcgg cgattgaggg
    34741 aaagtgcgct aaggcgggaa ctccatcccg ctcgaacgat ccgaggatgc gggagatata
    34801 cccctgcctg agcgtgagct cctcttgttc catagcaaac ggaagggagg cgagaaactc
    34861 tccgccctcc tcgctgagcc ggaaggagga gatcatcgtt tggactttgg cgaactcgag
    34921 atcgtcaaga ctcttcccag tcatactccg ctcctcgttc aatccatgct tcgctccgtt
    34981 catttctgga aagatagtcg ggggtgatca tctcaaggga ttcaacgatt ctcaggtagg
    35041 attcataccg atcgaagagg atctcctcct cttcaacggc aactttcact ccgcactccg
    35101 gctcctccat atgaaggcag ggatcgtaca ggcaccggta tcgcttgaac tcgggaaacg
    35161 cccgttggat ttgttgaagg tcggtgtggg gaacgaagat ctcgcggact cccggggtat
    35221 cgacgatcgt caggttcccc gtgcgcatca acagggagtg gttggtcgta tgccggccgc
    35281 ggtcatattt tgtcgagatc tcgtcggtct tctgatcgga tccaagaagg gcgttgatca
    35341 aggtggactt gcccaccccg ctttggccga cgaaagcgac cttcttgtcc ctgatgatct
    35401 gaacaagtcg atcaaggttt tctccggtct cggcgcttac cgccgcggtc tggaatccga
    35461 gggcgccgta atgctcaaaa cggaacgcat cctcctcctt catctcaaga tcgcatttgt
    35521 tcaagaggat gatcacctcc acatcgatcg ccgaagcgat cacccgatcg atgaagcggg
    35581 gtcggaaggg cggattggcg gcggatgtca cgcagatgat cgcatccatg ttggcgacca
    35641 ccgtctggtt cgccttgccc ttggtgttcc accgttgaaa ggagtttgtt cgctgcaatc
    35701 gcctgaggat cagaccctct tccgttccgg tgacgacaaa gcttacgcga tcgccgacgg
    35761 caagggggtt gtattcgccc tcaaccccct ccagctgctt tcccttgatg cggcagagat
    35821 actgctcttc cccgctctct acggtataaa tattattgat tcctcgaatg atcagtccag
    35881 tacgttcttc cataccttca ctgtacaacc ttctcttcac ccttgacaac cttccatttc
    35941 ttgatagggt gtgatcatgt attccttcac ccgctgcaaa gtgagctcct gcccccgtta
    36001 tgcgacccac atcagcgagt attgcctggc tcatgatccg aggcaggatc tcgcgcccac
    36061 cctctctttt cccgttctcg acagtgcgag tctgagcaat tggaactgca ccgaggaaga
    36121 tctttccggg aaacggattc cgggatcgct tttcagctac tcgacctttc acggggtttc
    36181 atttgtcaag acgaccattc tgaactccaa tttctcgttt tgcctctttg aggagtgcgt
    36241 ctttgatgaa agcacgatcc gctacgtgat cttcagcgga tcgaccttcc ttggctgcac
    36301 gtttcttaac tcctcaatca cccataccaa cttcaacgga tcggtcatga gggactgcga
    36361 cgcaagcggc agcgatctgt actattcctc gtttgcccac gcccatatcc acaacacgaa
    36421 gatggaggat tgcaacgttc ggaaggcgga ttttcgtcat accctccagg acaacctctc
    36481 cttccgctgg tccaattacc gggaggtgct gtgatgagga tccaccagca cttctccgtc
    36541 gtcggattct gcaacaccta cgtaatcggg gacaatgcca caaaggatgc tcttctcatc
    36601 gatcccggtc atgcggatat tgaattgatc cggctcttag agcagcatgg ctatacgatc
    36661 aaggccgtcc tcctcaccca tcgccacagc ggacacaccg aagggttggg gcttttgaaa
    36721 aagctctatg atttcactac gtatgcgtat gaagatcccc gccatccgat catccccaat
    36781 gaaatcctga caatcggtcc gtttgaaatc ttttcttatc tggtcggagg gcacagcagg
    36841 gattcggttg tgtataaaat caaagatgct ctgttcgtgg gcgatgcgct cagggcggcg
    36901 gccctcggaa aaacccatac ccaccgcctt gcggaacacc ttaaggatga attgaatcgg
    36961 atcttctcct ccctcgaccc gcaaaccctc atcttccccg gccacggtcc gccaagcacg
    37021 atccaaagcg agttgttgtt taatcagagc ttgagatagt cgaaaaacct tcgcggcagc
    37081 ggtgcggtaa acgtcattcg ttctcccgtc accggatgtg caaaggcgag gcttttggca
    37141 tggagcatca tcccctcttc gacttcccgg ccgtagaggg gatcaccgac aaccggatgc
    37201 ccgattgcat tgagatgcac cctgatctga tgggttcgtc cggtatgcag gacgagatac
    37261 aagagcgcac aatctcggaa ctggcgaagg acggtgtatt cggtcaatgc ctgacgccca
    37321 ttatcgtcgg aacacaccgc cattttcttt cgattgcttg gatcccgccg gatatgcaga
    37381 tcgatggtcc ctttggcatt cttcactttc ccggcaacca gggcgatgta gatcttgctc
    37441 gtcgatcgat ccttgaactg ggcgctcaat gctcgatggg cggcttgcgt cagggcgacg
    37501 accatcaccc cgctggtctc cttgtcgagg cgatggacga tgcccgggcg gacgccgtcg
    37561 ccgtgttcat cctgcactgc gagaatttct tctccatagc gatggagcaa tccgttggcc
    37621 agcgtattgt cccagtttcc ggcggcgggg tgaacgacaa gcccctgcgg tttgttgatg
    37681 acaaggatcg agtcatcctc gtagaggatc gaaaaatcga tctgttgcgg agtgagcccg
    37741 tcaaagaccg tcagggtgta ttcgatgcgg atttcatccc cggtctgaac gggattccct
    37801 ttcttcgccg gtttgccgtt cagccaaagc gcagcgttgg cgacaattgt tctggtaagc
    37861 tcttcatttt gagaagcaag atagagatcg agccgctgct ttccttcaag gtctgacacg
    37921 tgaaacgagg cggttttcgt gatcttcgcc attactgttt cagtccaagc gcccagtcgg
    37981 ctatcgaaac tcccaaagag agggttccgg cgatcgccgc ctgggtgaga aaatcattca
    38041 tcgcgctcgt ctgaggtggg atgattcccg ccgaacgggc cgcgctgtgc aagagaattg
    38101 aaaggggaag cgtgatgacg agggagccga agaaaatcgt ttccgccctg cgaatgttat
    38161 aggtccacag gggaaactcc ccttcttcat agggttcata gtccttgagc ggctcggaaa
    38221 agagactcgc tggaaccaga atgaggagaa tcatgaagat gatgatcggt ttcttcatcc
    38281 tatgttcgtg ctccaaaaag acgcgtgccg atccggacca tcgtcgaacc ctccgctatc
    38341 gccaaaacga aatcgccgct cattcccatg ctcagtgtat caaggttcaa gtcgggaaac
    38401 aattcttgag cccgatggta cagcttccgg gtttgttcga atgctttcct cgtaacggtt
    38461 tcatcactcg tcaacggccc gatgctcatg atccctttca ccgtgagata gcggaatgaa
    38521 gaaatcgcct ccagagcgaa gagcagttca tcttcatcgg cgaaaccgta tttgctctct
    38581 tcatcggagg ttttcagctg gagaagaatc ggataggggc gatccagctg cttttcaaga
    38641 atccgggcga ggcggaggga atcgatgctg tcgatccggt cgaacaacgt caacgcccgc
    38701 ttggccttgt tggactgcag gtgtccgatg agatgaacgt tcatcccttg ggggcggtcg
    38761 ccggggagtt tgtccgccgc ttcctgaact cggttttctc caaacaggag ctgcccttca
    38821 tcatacaggt cgagaatccg ctcatacggc tgggtcttgg aaacggccat gagggagatg
    38881 tcggtaagcg agcgaccgga cttgccggcc gcttcttcga tttcctgtct gatttctgct
    38941 aacgctgtca ttgaacgtat cataaagaat gaccggagag ggatttgaac ccccgaccag
    39001 ttgcgtgtaa ggcaaccgct ctcccactga gctatccggc caacagccgc gaacgtacca
    39061 cggaccgact tttcatgtca agctaacgat gatattcgtc gctttccttg agcagcacat
    39121 catgcgctcc gccttcgacg atgctggtcg ccgagacaag ggtaagctgg gcgttcttct
    39181 gcaggtcgcc gatcgtcaaa gaaccgcagt tgcacatcgt cgagcgaatt ttgctgaggg
    39241 taagctggac gttgtcccgc agttttccgg cgtagggaac atacgaatcc actccttcga
    39301 cgaaggagag ctgtccctcc ccttcataac gctgccagtt tcgggctctt gccgagcctt
    39361 cgccccaata ctccttcata tactggccct tgatcagcac cttttccgtc ggtgattcgt
    39421 caaatcgggc gaagtaccgg ccgagcatca aaaagtccgc gcccatcgcc agagcgaggg
    39481 tcatatgata gtcaaaaacg attcccccgt cgctgcagat cggaacatag atgccggtct
    39541 cttcaaagta ctcgtcccgc gccctggcga cttcaatcaa tgccgatgcc tggccgcgtc
    39601 cgattccttt ggtttccctg gtgatgcaga tcgatccgcc gccgattccg acctttacaa
    39661 agtcagcgcc gcactcggcg agaaagcgga acccggtgcg gtccacgacg tttcccgctc
    39721 cgacttttac ggtttcacca tacgttgacc gaatccaatc gagggtgatt ttctgccatt
    39781 cggtgtgtcc ttcggaagaa tcgatgcaca ggacgtccgc ccccgcatcc accaaagccg
    39841 gaacccgctg ctgaaagtcc cgggtattga ttcccgcacc tacgagatag cgcttctttt
    39901 tgtccaggag ctcaagcgga tgctttttat ggctctcata atcctttcgg aacacgaggt
    39961 atttcagctt ccgatccttg tcgatgatcg gaagagtgtt gagcttgttg tcccagatca
    40021 tatcgttcgc ttcgcttaag gtgatcccct cgaatccgta gatcaatgat tcaaaggggg
    40081 tcatgatatc cttaagcagc gtgtccggac ttgttcggct tggccgccag tcgcgggatg
    40141 agacgagtcc gagcagcctg ccgttgctcg tgccatcgtc cgtgatagcg atcgtcgagt
    40201 gtcctttttc ttctttgagg gcgatcagat cgccaagagt atggttggga gtgaggtttg
    40261 agtcggaggt gacaaagccc gctttatatc ctttcacccg tcgaaccatt gcttcctgat
    40321 cctgaatcga ctgcgagctg tagatgaagg acaacccccc ttcccgggcg agggcgatcg
    40381 ccatcttctc tccgcttacc gattgcatga tcgc
//
LOCUS       DAOSLI010000012        39214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1193_length_39214_cov_28.482673, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000012 DAOSLI010000000
VERSION     DAOSLI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 39214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39214
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1193_length_39214_cov_28.482673"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /gene="leuS"
                     /locus_tag="PLF67_03150"
     CDS             complement(<1..2270)
                     /gene="leuS"
                     /locus_tag="PLF67_03150"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOE89046.1"
                     /translation="MSKYPFSAIEKKWQAYWEENQSFAVTEDPTVPEEKRLYVLDMFP
                     YPSGSGLHVGHPEGYTATDIYCRYLRMNGYNVLHPMGFDSFGLPAENYAIKTGTHPKV
                     TTEHNIENFRRQIKSLGFSYDWSREISTHESEYYRWTQWIFLQLYKKGLAYEAHTPIN
                     WCDNCKTGLANEEVKDGKCERCGTPVVRKTIRQWILRITEYAERLLSGLDSLDWPESV
                     KLMQRNWIGRSEGATVRFRIEGMDESLEVFTTRADTLFGATYMVVSPEHPLVGELTTV
                     AQRPAVERYLDETSHKSDLERTDLAKEKTGVFSGSYAINPVNDERIPIWIADYVLISY
                     GTGAIMAVPAHDTRDWEFAKKFNLPIIEVLKSEIDVQEEAWVEDGLHVNSGFIDGLDK
                     HDAIEKMIAFLEERKLGERAVNYKLRDWIFSRQRYWGEPIPLLHCEKCGTVPVPEDQL
                     PLLLPEVTSYEPSGTGESPLAKIDSWVNTACPVCGGAARRETNTMPQWAGSCWYHLRY
                     LDPGNTERFTGEDAEKYWMPVDLYVGGAEHAVLHLLYARFWHMVLYDLGFVSTEEPYQ
                     RLINQGMITSYAYQRKDKSLVPSDEVTEVAPDAWIETATGESLERVIAKMSKSLKNVI
                     NPDEIIHEYGADSMRMYEMFMGPLEVSKPWSTAGLVGIYRFLDRIWRLYDEVDIVDEA
                     PNDELLRTLHKTIKKVTEDTRDLAFNTAISQMMVLVNELYKESVFPRSVAETFVKLLS
                     PYVPHLAEELWERLGH"
     gene            complement(2299..3552)
                     /locus_tag="PLF67_03155"
     CDS             complement(2299..3552)
                     /locus_tag="PLF67_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE89047.1"
                     /translation="MNISLAVVFALLVLSALFSATETAYTSLSFFQLKTLESRKSRAG
                     KLAYALSQDKDHLITTVLIGNNIVNLSASALVTTITIKYFSSTLVGYATGILTFLILV
                     FGEIVPKRLALVHNVRIAILMAYPMKALMLLLFPLAWLLQVLSSAITRLFGTKEEPVI
                     TTEGVMHVVDAAEDVGLVDQYESDLMQRAIHFSSTTVRTIMTHRTEVFTLPSDLTIEE
                     AFPKIIKSGFSRIPIYQDNQENIVGILLLRDVLRAQQKQVVNKTLASLSRKPIYVVEQ
                     MHLDDLFFLFKKDKLQQAIVIDEYGGFSGVVTMEDVAEQLFGELFDEHESRFPDRVVQ
                     HKDKPGTFVVMADAPFQQVVDDLDLWYEGDRVSTVAAYLLQEAGSIPVEGEVITTDLG
                     TWQILLMKGNKVEVVEFTPNPASVD"
     gene            3771..4028
                     /locus_tag="PLF67_03160"
     CDS             3771..4028
                     /locus_tag="PLF67_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89048.1"
                     /translation="MKDNNTVICSFSMDRNLYNAYKNIVTQNGENVKDNLVRYMQQVV
                     DYEIPNAETIKALEEVKRLKADPHKKTYGSFAEIMEELYDA"
     gene            4064..4246
                     /locus_tag="PLF67_03165"
     CDS             4064..4246
                     /locus_tag="PLF67_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HOE89049.1"
                     /translation="MSKKPSGEPLPKKYRDHGLSGNDKGCRECHIAPDWLLIYEISGS
                     ELILYLTRTGTHSDLF"
     gene            4325..6127
                     /locus_tag="PLF67_03170"
     CDS             4325..6127
                     /locus_tag="PLF67_03170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOE89050.1"
                     /translation="MGVALYDESKILTLSALEHIRMRPGMYIGRLGNGSHIDDGIYIL
                     IKEVIDNSIDEFIMGHGSKIVIRLKDNQVRIRDWGRGIPLGKVIECVSEINTGAKYND
                     DVFQFSVGLNGVGTKAVNALSSDFEVTSFRDGKFTNAVFKQGILVKETSGKSDEANGT
                     EVIFTPDEELFGDYAFNEDFIIARIWGYAYLNTGLSLEFNGRTFRSSKGLGDLLAKEV
                     ADEGLYTTIHHKGERLEFAMTHVSGTYGESYFSYVNGQHTSDGGTHLSAFKEGIGKGI
                     SEHFKKNWSPQDVREGIIGAIAVKIKSPIFESQTKNKLSNTEIRIWIVNEVKDAIIDF
                     LLKNPKDAEKISRKVMQNEALRKELNEVKKGARESARKVSINIPKLKDCKYHLGQSAA
                     HKEKCDNSMIFITEGDSASGTITKTRDVLTQAVFSLRGKILNVHGKKKTEIYKNAELY
                     NLMVALGIENGIEDLRYGKVIIATDADTDGYHIRNLLMTYFLTYFEDLVLSGRLYILE
                     TPLYRVRNRTTTVYAYNEKQRDDAVARLRGAEVTRFKGLGEIDPREFGQFIGEDMRLI
                     RVSLPGVTEMHRSLEFYMGSNTPERRSFIMENLI"
     gene            6127..8106
                     /locus_tag="PLF67_03175"
     CDS             6127..8106
                     /locus_tag="PLF67_03175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOE89051.1"
                     /translation="MTQAHQLFRQNFLEYASYVIKERAIPDLVDGFKPVQRRIIHTLF
                     EMDDGKFHKVANTVGWAMRFHPHGDASIYEALVNLANNDLFIDRQGNFGNILTGDSAA
                     APRYIECRLLPFAKEVLYNPELTEWVESYDGRNKEPVVFPAKIPVVLIQGVMGIAVGM
                     STLILPHNRFEVLDAMAAHLRGESFELFPDFPQGGIVDVEDYNDGKGTVLVRARLNTS
                     DPKRIIVEELPYGTTSESLINSIEDAAKKGRLKISSINDFTAESVNIEINLARNTYSK
                     DIVDALYAYTDCEKRITTNPLVIRDNVPTVMGVSEILAFHAEHLKVVLKAELELEEEK
                     LLAKMHDRTLERIFIEERIYKRIETKRSADDVLKAVHAGLKPFSSELLRDVTDEDVER
                     LLKIPIRRISLFDIERNRQEIEQINEQLEEVRSKLENLTGYALETIDRLKGMFDRKEH
                     KRRSTLKKFELVDVKEVAVRDLVVRYDSTNGYLGYGVKTGAEQVTVSQFDRLLVIRRD
                     GSYQVIDAPEKEFVGKGMLHCMIADRDELSKIVFTLIYQEKTYKYTFIKRTQITSFQL
                     KKLYPLLPDEKNYRVVRLLTHPNAEIGVTYKPKSGLRILEEKFYLSDFLVKNPRAKGV
                     RMTVKEIASMRIRSVKGDVSSAKDPELFDEDEEEA"
     gene            8103..8795
                     /locus_tag="PLF67_03180"
     CDS             8103..8795
                     /locus_tag="PLF67_03180"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE89052.1"
                     /translation="MRSFSERILYEDNHLLIVNKRCGELAQRDQSGDPTLGDFAKEYL
                     RVTYDKPGNIYLGIPHRLDRPTSGAVVLAKTEKALIRMNTLFREDKVDKTYWAIVDKA
                     PPEIEGTLVDNLVKDAKTNKSRAFPADSKKGKRAVLSYRHRASSKNFHLLEITLETGR
                     HHQIRAQLAAHGIHIRGDLKYGYPRSNRDGGISLHARTLSFIHPVKQERITIVAPVPD
                     DPLWKVFEEMTT"
     gene            9021..9935
                     /locus_tag="PLF67_03185"
     CDS             9021..9935
                     /locus_tag="PLF67_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE89053.1"
                     /translation="MEPPTIRQKMAKHWQFYLIVSLPVLYFLLFKYLPMYGILLAFKR
                     YRVSMGIWRSPWAGLYQFKKFFNNPSALRVMYNTLVLSVYALVTSIPLAVILAIALNE
                     TGSKLWRKTVQMVTYAPYFISTVVMVAILMQFLDPALGMFNTIRNLFGKESVNFMGEA
                     KLFKHIYVWSALWQNTGYNAIIYLAALTGINPELYEAARVDGVNKFQKIWHVDLPSIK
                     TTIVIMFIMNMGFIMSLGFEKAFLMQNPLNLESAEIMSTYVYKMGLINSDFSYSTAID
                     MINSVINIILILTFNKIAKMRNEGGGLW"
     gene            9935..10819
                     /locus_tag="PLF67_03190"
     CDS             9935..10819
                     /locus_tag="PLF67_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89054.1"
                     /translation="MKKHLASDRIFDLLVNIFLVFSFLIVLYPLLYVVSSSLSHPTAV
                     MANKVWLLPVDFDLMSYKAVFTNKQIGTGYLNSLYYMVVGTVVSVLLTMLLAYPLSRK
                     EFYGRSVVTKFILFTMLFSGGTIPLYLVVRNLGLYDSRWSIILPNAITVWNVIIARTF
                     LQENITDELYEAAQIDGCSDIRFLISFVFPLSGAIVAVLGLFYAVGQWNKYFDALLYL
                     QNQALYPLQIVLRNILIINRNTPSMTVDVEAAIRAQGLSETIRYAVIVVASAPLLLIY
                     PFVQRFFVKGVMIGSVKG"
     gene            10866..12563
                     /locus_tag="PLF67_03195"
     CDS             10866..12563
                     /locus_tag="PLF67_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89055.1"
                     /translation="MRKSILIVLFALLLPALLFAQGSKREPEKPAVEYTPIGTFPIVK
                     EKITVDIMVVQPPCVEDYNTNRFSKYMEELTGIHVNFIMVPEQARQEKLALVLASGDY
                     PDAFLGFGIGTELETTYGSQEGLFLPLNKYYSKEWMPNMMKAFEEFPGGIGYMTNIDG
                     NIYSIPRLEGCYHCSNMAKMFVYQPFLDALGLKVPTTTDEFYRTLKAIKERDPNGNGR
                     ADEIPLAGSIIGWGDQVERFLLNSFIYCDLDTNINANAESNVGFMMVDGKVDTAMNKD
                     GYREGLAYVHKLYKEGLIYSGSFTQDSSQLTQLVESSAQPTVGFATGGWRGQFATIGG
                     DRFNNFKAIAPLKGPKGVQYSVVFFQDPSMGSLVLSSKTPYADAIMRYFDFMYTQEGT
                     LMQRNGFQGEAWDWAKPGQVGLDGKPAVWEQLKLWNDKDPQNDTWIQTYASATTPSLK
                     NGLASPPITPEDPEYYLPSNNEKTLFDETTYLYKPYEDVTVEVPHLKYTAEENEQFST
                     VKRELANYIRQSAVKFMVGSMDVHNDRVWNDYVAGLERLQMSNVLALMQKAYDRQYK"
     gene            12570..14027
                     /locus_tag="PLF67_03200"
     CDS             12570..14027
                     /locus_tag="PLF67_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOE89056.1"
                     /translation="MSGVDKPNVVYLLFDDLGYGDVSAFNPKAGFKTPRIDRLAKMGM
                     CFTDAHATSAVCTPSRYSIITGRYNWRSTLKSSVLGGYSKPLIAPARRTIANLFRDIG
                     YRTHAVGKWHLGLDLPTGEGFVEAPGFAQSGPLEHDRPIGGGPTALGFDTFYGISASL
                     DMPPYLYIRNDRFTSAPTRTTRSDGMGYWREGPTGEDFIHEEVLDHLTGETLRIISSG
                     DERPFFLYYALTAPHTPILPDREFRGRSGTNAYGDFVLHCDDVVGRILDAVDENTVVV
                     LTSDNGCSPQADFEELRRFGHHPSHHFRGMKSDIYEGGHRVPYIIVWPGVTRADTVCD
                     RTVSLVDLYATFAEYFGLSLASDEAEDSVSMLNLLEDPDSEAKRTSLIHQSIDGSLSI
                     RKGPWKLELCPGSGGWSPPVPGSAEEKLLPAMQLYNLDEDIGETTNLIDRHPEVVRRM
                     KAELLTIVRDGRSTPGANQPNEGAAFWETAAYLRP"
     gene            14140..15438
                     /locus_tag="PLF67_03205"
     CDS             14140..15438
                     /locus_tag="PLF67_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HOE89057.1"
                     /translation="MTTRRPALHFSARKGWINDPNGFCFFGGRYHIFYQHNPYAPQWG
                     QMHWGHAVSDDLVFWKELPIALVPDRWYEDDPKGGCFSGSAIVIDGILHLYYTAAAGG
                     HQRQCVATSSDGVTFVKAERNPILEAPSGFEDFRDPKVFFIEERLFMVIGASDGTEHV
                     VLLYEGSDPYTWEERGVLYSVPREIGTMPECPDFFPLDDKWVLVFSPIGKDTHGVVAV
                     IGTFDVHTCSFTSESIQPLDHGSHYYAFQSTSGNDRKRYAFAWAGQWPWMGRFSGHGS
                     DAEGWRGFLNFPRRLRYVDGRIHSYPVEGIRDLFTDSLIDKSLKPNCDLSLTSLGGVY
                     LMSITYSSSFSLRFGSTQLAVDSVSHTATFTHHGTTGIAPLAGGLVEIFVDRMSTEIF
                     FDGGYASICFNRHEDEDDTMMISNGNAPLKLSASGSPLHR"
     gene            complement(15407..18337)
                     /locus_tag="PLF67_03210"
     CDS             complement(15407..18337)
                     /locus_tag="PLF67_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOE89058.1"
                     /translation="MYTLDPTWLPFSNLMLNHIYNVLLICNDYDRFLLEEDGRVEEEL
                     YIEYTQLRLSNPPKITYASGGEEALELLETRTFELVITMLDLGPESVENLASSIKQVD
                     PGMPIIALSPSSGHRRNKLIKGSDSIAIDYFFYWQGDPTIFLAMIKLVEDRMNLDHDI
                     AEADVGLIILVEDSVRFYSSYLPMIYTCLIQQTQAMILEALNTWGKTLRTRGRPKIVL
                     AKNYEEALALYEKYQQHVLGVITDVSYEMEGVHNKHAGFALAKELHSRNGELPILLQS
                     NDLSMKEAAEKTGTHFIYKRSSSLFTQLASYMRTNYGFGPFIFRNPATGAEIARVETM
                     RALQHILPEIDLASFEYHFRNEHFSRWLKAQSLYTIAAKIKALKIPEGGDVALIRDEM
                     IETIRSYRAERTKGVIAQFSPANYDETLFFSRIGTGSLGGKGRGLAFIDMVLRQAGIP
                     AKYPDIYLSIPRSVVIATDQFESFIEANDLKDITTADLPDDTLLSIFLSKPLAEDLIE
                     NLKHILSVIKQPICVRSSSLLEDSHYQPFAGVYETVMIPNRGSEELRLEELSNAVKSV
                     WASTYFRRAKEYVKATDYLLGDEKMAIIIQQVIGSQHGSYWYPNIGGVARSINYYPPS
                     GEKPEDGVGLLSFGFGKAVVESGSALRFSPTHPKRPSQFLGGNQSSSQERFYALDLRE
                     GFVPRLDRLDNLKLVDLSEAEAHPESLRYVASTFDFANQTFNEAAGSVGKKIITFNGV
                     LKYDAFPLAPVVRDVLRVGSEAMGRPVEIEFAVNLNRKAPKKPEFSLLQIRPIAQGSE
                     EIDVSIEDDDRKAALILSTSVLGNGTLDSIEDLICVKLDAFDPSKMVEMANELDELNT
                     GMVLSERDYVLVVAGRLGSSDPWLGIPVSWSQISRSKVIVETGLKDLRAEPSQGTHFF
                     HNLTSLGSFYLTINPESGQGTLNVRRLRELPVIKETKHFIHARAPLIVKVNGFSGEGV
                     ILTR"
     gene            18534..19871
                     /gene="gdhA"
                     /locus_tag="PLF67_03215"
     CDS             18534..19871
                     /gene="gdhA"
                     /locus_tag="PLF67_03215"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOE89059.1"
                     /translation="MYNLSDFMNELERRNPAQPEFIQATREVLESIIDAVNGNPVYEK
                     HRILERLTEADRIFSFRVEWEDDEGNLQVNRGYRVQFNNALGPYKGGLRFHASVTLGT
                     LKFLAFEQTLKNSLTTLPMGGGKGGSDFSPKGKSDGEIMRFCRSYMSELQKYIGPDTD
                     VPAGDIGVGAREIGYLYGQYKRLRDENTGVLTGKGLNYGGSILRPEATGFGAMYFANS
                     MLALRGDSLEGKTVALSGFGNVAWGAAQKATHLGAKVVTISGPDGYIYDEEGVGTPEK
                     WAYMSYLRLSNNDVVEPYAKEFGAVFVPRKKPWEVKVDFAFPCAIQNELSLADAKALV
                     ANGVQGVVEISNMGCQADAMYYFLEKKILFAPGKAANAGGVAVSGLEMTQNAMHIAWT
                     AQEVDEKLKEIMLKIHNACVKEGTQPDGYINYVKGANITGFRKVADTMVQLGY"
     gene            20265..22769
                     /locus_tag="PLF67_03220"
     CDS             20265..22769
                     /locus_tag="PLF67_03220"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOE89060.1"
                     /translation="MNAGKNELTALGRKIFLDRYALKDVKKETLAVGDIVVAVSNPQT
                     GQREIGTVTAIRDGDEIVVTLDDGVVLEVKREEIDKPIETEPGQMLNRVAKGIAAQEK
                     KEIRSKWEKEFNWILEDWRFVPGGRILTGAGTDQNLTYFNCYVIPSPKDSRGGIMTSL
                     GQMTEIMSRGGGVGMNLSSLRPRHAYVKGVNGRSSGSVSWGGLFSFVTGLIEQGGSRR
                     GALMLILNVWHPDIMDFINSKREMGKITNANISVGITDEFMAAVRSDKEWNTVFPDTS
                     DPKYNSDWDGDLEAWIKKGGSTVVYQTLKAKEVWNAIVESAWASAEPGVFFMDRYNQM
                     SNSWYYSSIQSTNPCGEQGLPPWGVCNLGSINLSQFVKDKRVLWDDLGRTVRSSVRFL
                     DNVIDDTPYFFEENRKQQQGERRIGLGTMGLADMLIKMELAYGSEPSLKFIDELYKFI
                     CLEAYDESCNLAEEKGSFPFFDAEKLLQSGFMMQMPEEIREKVRTHGLRNVTLLTQAP
                     TGTTGTMVGTSTGIEPYYFWEWERTGRMGTNIERVKVYEDWVKANPGKEKPDYFVSAM
                     DLAPEGHVYVQAAIQRWVDSSISKTGNTPKEYTVEQTGKLYELLYDTGCKGGTTYRDG
                     SRDTQVLTIKKDEEEEKKETVLSFTDPKPRVRATVLRGTTYRKATPIGTAYITVNCDG
                     TDPSDIFEVFINVAKVGSDVAADAEGLGRLISLILRMPSPLTPEQRAQTIITQLQGIG
                     SGRSMGFGKNRVMSLPDAVAQVLEEHLGSTPSERPPMNLPDEEDEEEGEGQLFLSLSS
                     SKPGSIQADICPVCGNGTFMNIEGCRKCFSCGHSEC"
     gene            complement(22825..24291)
                     /locus_tag="PLF67_03225"
     CDS             complement(22825..24291)
                     /locus_tag="PLF67_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOE89061.1"
                     /translation="MKGRIFVGGLHHESDTFNPILTSREDIRVTRREELLVKRESSAS
                     GIIQTLIAAGYEVVPSLVARAVPNGVWERSCYEELKEELLTDLRNAGPLDALCLPLHG
                     SMRVEGIGEAEQDLLSSIRAIEPSIPIFASLDMHATLTKRMKSLVSGFVGYKCAPHTD
                     TYETGIHAALMTIRTLESKKRPTMVAVRIPMLIAGEQSETGVEPMRSLIGELRRRERR
                     SSVLACSYLLGFPWADGTENGVHAVVVTEENQALADELARELADLFWAKRNEFTFYTE
                     THLPEEAMAATKRSLAEGIHPVVISDSGDNPTAGSSGDVTGFLHLLLKDPELSRLDPP
                     LIYQGFYDPQIVAEAEKVGVGEVIESRIGSRFDPAKSSPVAGSFTVKALFNRWEHGTR
                     LALLHIGGIDMVAMTKHVGCYDPEMMRALGVEVENRKVIVVKLGYLEPEIRAIARRSM
                     MALTTGSSDELFERLEYRNLPRPIFPLDGEFEAELELI"
     gene            complement(24288..25616)
                     /locus_tag="PLF67_03230"
     CDS             complement(24288..25616)
                     /locus_tag="PLF67_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOE89062.1"
                     /translation="MQTTIQSWRKLGFGLFIHVGLYSILGGVHQDRKVIRGYSEQILS
                     HGYLPQWDYERLVDRFTFERFDPDHIVAVAKRAGMRYLVITAKHHDGFCLFDTKSTAY
                     NSINTPYGKDAVRELSEACRRGGLEFGVYFSWIDWNFPHALPISPHNCDAIPPAHQRL
                     NIAQLTELLSGYGPIRELWMDMGAPTAEQSAEVYALVKSLQPGCAVNGRIWNDYQDFL
                     TMGDNQIPTLPLNCPWQTPASIYKETWGYRSWQERGDLEDKVTELTETLRSVRAAGGT
                     YLLNIGLKGDGSIVPFEEEVLLGIGKRLREKPLEAEDPRLPSADLPLGTPTFGSVHYR
                     YGGTDYYTYKPIPTTLAWKVALEEHGTYSLVWEHEPPLEEAVKVQIRLDELLWHATLQ
                     AGQRSGILAEELHLQSGTHQLEVSTVGEPMKRTELTCPDLMMTIKKEHKE"
     gene            25961..29497
                     /gene="nifJ"
                     /locus_tag="PLF67_03235"
     CDS             25961..29497
                     /gene="nifJ"
                     /locus_tag="PLF67_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOE89063.1"
                     /translation="MSNKRKVTIDGNTAAAHVAYAFSEVAAIYPITPSSTMGEFADEW
                     ASVGRKNMWGKTVEVVEMQSEAGAAGAVHGSLAAGALTTTFTASQGLLLMIPNMHKIA
                     GEMIPTVFHVSARSLAAQSLSIFGDHSDVMSCRNTGFAMTCANNVQEVLDLGLVAHLS
                     TLEAQVPFLAFFDGFRTSHEVQKVDELTYEDMYTLVDEKYVRRFRDRAMRPEKPVLRV
                     AAQNEDVYFQGRETVNKYYEALPEIVQKYMDKVAKLTGRTYHLFDYVGAPDATDVVVI
                     MGSGADTVEWASDYINERGGKTGVLKVRLYRPFSAKHFVQALPASVKRVAVLDRTKEP
                     GSLGEPLYLDVITALNQMDRTGIKVIGGRYGLSSKDFSPTHAKAIFDHLAGPAFSNFT
                     VGIKDDVTNRSIEIKEQINVIPKDVVSCMFWGLGSDGTVGANKNSVKIIGDNTDLNAQ
                     AYFSYDSKKSGGITISHLRFGKSSLEMPWLVDHADFVACHNPAYIGRYDMLAPLRKGG
                     VFLLNSQIPSDEVFDHLTREMQEQIIEKEIRFFNINALEIAEGVGLGSRINTVMQAAF
                     FKISEVLPEEEAIALIKEYVKKTFLRKGEDIVKMNWAAIDGASDALHRVEIPKTITKS
                     YEPVPLIPEDSDRFSKKIIEPIMHLKGNDIPVSEMSFDGTLPTGTAKLEKRGVAPFVP
                     KWIAEHCIQCNQCVQSCPHAAIRAKQIEPERLKDAPETFKVLKSISKNDRDLQFKIQV
                     YTEDCQGCGVCIETCPSKVKSLEFSPIQVERDAGEIANAEFFEQLPYDVLDGSPINSI
                     KGLQFKQPLFEFSGACAGCGETPYVKMVSQICGDRMIVANATGCSSIYSGTFPTTPYT
                     VRQSDGQGPSWGNSLFEDNAEYGLGMRLAVDANREQLKIYIDKLFALGTSAELKSAIE
                     KSLELWKESSEEANDAQKAVKAALPGAIAAAKSDEERKLLGKIDELKDYFADKVIFII
                     GGDGWAYDIGFGGVDHVLASGRNVNILVLDTEVYSNTGGQASKSTPIAAVAKFANAGK
                     EIGKKNMGFMAMTYGHAYVASISLGANRLHAQRTLQEAVAFDGPSIVFAYATCINHGV
                     NMRYSQEIMKDAVNSGYWPLYRYNPSLEEGKRFSWDVRSTPGSFQDFIRAEVRYSSLF
                     KTNPKNAEALFARAEEDAKRRMEFYQKLGPLM"
     gene            29689..30867
                     /locus_tag="PLF67_03240"
     CDS             29689..30867
                     /locus_tag="PLF67_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE89064.1"
                     /translation="MDAAERYVKRILDISAPLRRKSQFLFGPRMTGKSTLIEKELVEK
                     PALYWNLLRTGLQMRVKQNPELLTQEVEARQLSHCLIVIDEIQLVPELLDEIHFLIEE
                     RSIRFLLSGSSSGKLLREKGPNLLGGRAGNLSLHPFVYPEIKEMGVTLEHIFTTGLMP
                     HVLLSDDPDQDLDDYIAVYLRDEIGRGGNVRKLETFARFLDVAALTNAEQVNYMNRAG
                     DVGISRQSIANWYQILIDTLIGHYLPAYTKGVKRKTFSLPKFYLFDLGITRALLQIPP
                     PVEGQSEYGRFLEHYIFMELKAYLDYGRRKEILSYWRTTGGIEVDFILGDRIAIKVKA
                     TKQSNARDYKGLLAFMEEGIAERHILVCREEHPRNLKNGVEIHPWTYFLEQLWGGKIL
                     "
     gene            complement(30898..>31170)
                     /locus_tag="PLF67_03245"
     CDS             complement(30898..>31170)
                     /locus_tag="PLF67_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOE89065.1"
                     /translation="YPKTDNAIKPTPRCPVDSIFMKDSYREPHLKEMEYAYFEHEEQK
                     RRIQSLPYENTGTLADFYYRRKHTSAFMREMNRSTEVMFRRWVEEK"
     assembly_gap    31171..31270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31271..31482)
                     /locus_tag="PLF67_03250"
     CDS             complement(<31271..31482)
                     /locus_tag="PLF67_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE89066.1"
                     /translation="MNETLRLIHNRRSLRAFSDKPIPPELLLALKEATLRAPTAGNMA
                     YYSIVEVCDREKKEKLALLCDNQPMIA"
     gene            31565..32830
                     /locus_tag="PLF67_03255"
     CDS             31565..32830
                     /locus_tag="PLF67_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE89067.1"
                     /translation="MRSVRVYRYRWVVLISLINLVLAGSLQWLNFAPVGRIVNAYYRD
                     QILLRFFNPIDLLSLTYLIVYVLFSVPSGWIIRKLGMRVTYWIASGLIIFGAMTKTLY
                     LSSFKTVLFGQILLGLAQTFTLTSITETTSRWFPIRERGMAVGIIQASQYLSLALVMI
                     LSPLFVVRSQKGIEQLMRLWAIISIVLAAVSAALVRERPPSPSSTFEREEEMKFSQAL
                     STALSRRSLRILIMEFATSWAVLMTVFIKIDEISEFLGFVNSNGILAVTMLIFGMVSA
                     VVIPALSDHFRKRKIFYTLCALTAVPGSLLLLSGNITVALIGAAILGSGLLASIPIGS
                     QYATELGHGINEKVIQSILQFFSQLATAVILFVTTIIKERYQLVLPAILATILLGSLI
                     ATFFIKESEVIITEDERLKALIDLENVHH"
     gene            complement(32832..33404)
                     /locus_tag="PLF67_03260"
     CDS             complement(32832..33404)
                     /locus_tag="PLF67_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE89068.1"
                     /translation="MPVLSILQTRRAYRAIDSKPIDEEVLTRLVEAAVTAPSANNSQP
                     WRFITVSDPAVLAEVKNTLTPGNYWAQNAPAITAVVTNNDWSIIIGDRVFAPFEAGMA
                     TMAYQAQAISEGLHIHPIAGFNADAVKEALKIPASEMLLTLLIIGYPGDDSTLKEHHL
                     KSERSPRVRLPLDHVYAKDRWNDRLLPQKP"
     gene            complement(33508..33666)
                     /locus_tag="PLF67_03265"
     CDS             complement(33508..33666)
                     /locus_tag="PLF67_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOE89069.1"
                     /translation="MQYECNLCGYIYDPAVGDPDNGVAAGTAFADIPEDWVCPLCGAP
                     KSDFSPLD"
     gene            complement(33726..34691)
                     /locus_tag="PLF67_03270"
     CDS             complement(33726..34691)
                     /locus_tag="PLF67_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOE89070.1"
                     /translation="MKGERVAQIELLLHSHPEGLRRAEIARRLGVHRSTISRYIDKLA
                     EYIDLYEENNLLKIRAKDGEEVHALSVYESLAINLSAEMLLNTSEFQNPHLASGLRKI
                     AMNMRSYAPKISENIVSLAEQIDKQLIAQKESSAFNAILEVLIDSWVSGRIVRIVQSL
                     KGFDSIETELAPYFIGFRETDGTRHPITVTGRLRHTTEIVTVDISTITSATILDETYT
                     IPDNLKPFKFPDAPETFDAIDMIPLSLKLKERSAMNAFRQLVHGKPQLEKLKDGSWIC
                     NMDVENSIELTLRIIQCGDAVEILGPEHYRRRFMKRLEDVLAIYR"
     gene            34849..36024
                     /locus_tag="PLF67_03275"
     CDS             34849..36024
                     /locus_tag="PLF67_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOE89071.1"
                     /translation="MKISAIGCCLIDYLFHEGTYSKSVFNEYRSVVSGDGGLLVGGLV
                     FGEQLAEFTGLPFDTLLRRLIPHPEEGVENIGGPAIIALIHAAQLLYDSPHSFHFHGV
                     AGDDVEYRLIREHIAKTVIDPRFLILEGERTPTTYVLNDRHAHGGKGERTFVNLLGSA
                     AHVIEADVGDDAYRSDIVLFGGTALWPAFHTRIDQALRRAKAEGALTIVGTVFDFMNE
                     SRSSSEAWPFGEESGYPFVDLLVCDALEAERMSGQRDLEKAAAFFDEAGVGAGIITHG
                     SKEIIAWSKKESVFGHTPLTHFPVCAHIDELLERDPSLRKDTTGCGDNFLGGVLASII
                     AQGAKKCSMVDAITLGGASGGFTLTHHGGMFHETYPGEKRSLLEPVLAAYSGQLGVL"
     gene            36032..36226
                     /locus_tag="PLF67_03280"
     CDS             36032..36226
                     /locus_tag="PLF67_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HOE89072.1"
                     /translation="MDDRLINVEMQLTFAQETIETLNGVVTEQGMELQRLKTLVEKLE
                     RRIVDLVEGSEGGPQKPPHY"
     gene            complement(36223..37896)
                     /locus_tag="PLF67_03285"
     CDS             complement(36223..37896)
                     /locus_tag="PLF67_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE89073.1"
                     /translation="MATYLIVGGVAGGAGVAARLRRRDEKARIIIFERGEHISFANCG
                     LPYYAGGVIENRSSLFVMTPELFKRVLNVEARVNSEVMSVDRAQKLITVRNVKDDTTY
                     TQAYDHLILSPGASPIRPRIPGIEHPSIMTLRSVKDIDRIKASIDRSETKRAVVIGGG
                     FIGLEMAENLKHRGLEVSLVEALEQVMGVIDYDFAAQVQQHMRKKGVVLHLKDPIKGF
                     SKNGEAVLVELESGKVLETDLVILSIGVRPDTTFLKESGIVLSSTGAIVVDEYFTTND
                     PSIRALGDAICFACPLTSHSITVPLAGPANKQARLLADALIDGNRKPYKGTIGTSIAK
                     VFDLTVASTGLAEKTLAALELPYKSAVTHTSSHADYYPDSLSLSLKILFHPTTGKIWG
                     AQAIGYEGVDKRIDVISSFIGMDGTVYDLAEFEHAYAPPFSSAKDPINMVGFIGMNAL
                     EGISNPISWREVDNLIEQGAVILDTRSEMETMTGGMLPGSINIPHTALRNRIAEVPKD
                     KPIITVCVMGTRGYLAERILRGNGFTEVYNLTGGLRSYTTAISERARLN"
     gene            complement(37881..38246)
                     /locus_tag="PLF67_03290"
     CDS             complement(37881..38246)
                     /locus_tag="PLF67_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOE89074.1"
                     /translation="MLNLCAIRRLQSALRSFEERLKDETGLSFNDALLLCAVEKGIVE
                     PSALAKELDLSPSRLTRVIDSLEGRSLVQRTLSITDRRSLVISLTEAGEELVLAYNCC
                     ELQIPEELAFTQEESWQHI"
     gene            38709..>39214
                     /locus_tag="PLF67_03295"
     CDS             38709..>39214
                     /locus_tag="PLF67_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE89075.1"
                     /translation="MKTILCYGDSNTYGSNPGGGRWEYDKRWPGILSSLLGDGYYVIE
                     EGLGGRTTVFDDPFEKGRNGKTFLPVALASHQPIDLLIISLGTNDTKRFFGSNERIIT
                     RGIEALADVAKTFDVKQIMVISPIHVGERIEETVFAGFSRESVEISCKLSERVKAMAE
                     RKGLIFFDA"
BASE COUNT         8596 a        10875 c        11227 g         8416 t
ORIGIN      
        1 ttgtgaccga gcctctccca gagctcttcg gcgaggtggg gaacatacgg cgaaaggagc
       61 ttgacgaagg tttcagcgac gcttctgggg aagacgctct ccttgtagag ttcgttgacg
      121 aggaccatca tctggctgat cgcggtgttg aacgccagat cgcgggtatc ctcggtaacc
      181 tttttgatcg tcttgtggag ggttcggagc agttcgtcgt tcggtgcctc gtcgacgatg
      241 tcgacctcgt catacagccg ccagatccga tccaggaagc ggtaaatacc aaccagaccc
      301 gcggtcgacc atggcttgga aacctcaagc ggccccataa acatctcata catccgcatc
      361 gaatccgccc cgtattcatg gatgatctca tccgggttga tgacgttctt caggctcttg
      421 ctcatcttgg cgatcacccg ttccagagac tcgccggtcg ccgtctcgat ccacgcatcg
      481 ggagcgacct ccgtcacttc atcggagggg acgaggctct tgtctttgcg ctggtacgca
      541 tacgaggtga tcatcccctg gttgatgagc cgctggtagg gctcttcggt ggagacgaag
      601 ccaaggtcgt agagaaccat gtgccagaac cgggcgtaga gcaggtgcag taccgcatgc
      661 tccgcccctc cgacgtacaa gtcgacgggc atccagtact tttccgcgtc ttctccggtg
      721 aagcgctcgg tgttgccagg atcgagataa cgcaggtggt accagcagga tcctgcccat
      781 tggggcatcg tgttggtctc ccgtcttgcc gctccgccgc atacgggaca cgcggtgttc
      841 acccaggaat cgatcttcgc caaggggctt tcgccggttc ccgagggctc atagctcgtc
      901 acttcgggaa gaaggagggg aagctgatcc tccggtacgg ggacggttcc gcacttctcg
      961 cagtggagca gggggatcgg ttcgccccag tagcgctggc ggctgaagat ccagtcccgc
     1021 agcttgtagt tgaccgcccg ctcgcccagc ttccgttctt cgaggaaggc gatcatcttc
     1081 tcgatcgcat cgtgtttgtc caatccgtcg atgaacccgc tgttgacgtg gagaccgtct
     1141 tcgacccacg cctcttcctg cacgtcgatc tcgcttttca gcacttcgat gatcgggagg
     1201 ttgaacttct tggcaaactc ccagtcgcgg gtgtcgtggg cgggaaccgc catgatcgct
     1261 ccggtgccgt agctgatcag gacgtagtcg gcgatccaga tcgggatccg ctcgtcgttc
     1321 accgggttga tcgcgtacga tccgctgaag acccccgtct tctcctttgc cagatcggtt
     1381 cgctcaaggt cgcttttatg ggaggtttca tccaaatacc gctcaacggc cgggcgttgt
     1441 gccactgtag tcaattcgcc gaccagcggg tgctcggggg agacgaccat gtaggtcgcg
     1501 ccgaaaaggg tgtccgcccg agtggtgaac acctcgaggc tttcatccat cccctcgatc
     1561 cggaagcgaa ccgtggcacc ttcgctccgt ccgatccagt tgcgctgcat cagcttcacc
     1621 gactcgggcc agtcaagaga atcgagtccg gagaggaggc gctcggcgta ctcggtgatc
     1681 ctcagaatcc attggcggat cgtcttccgg acgaccgggg ttccgcagcg ttcgcacttt
     1741 ccgtctttga cttcttcatt ggcaaggccg gtcttgcagt tgtcgcacca gttgatcggg
     1801 gtatgggctt cgtacgccag tcctttttta tagagctgca ggaagatcca ctgcgtccag
     1861 cggtagtatt cgctttcatg ggtcgaaatc tcccggctcc agtcgtagct gaagccgagg
     1921 ctcttgatct ggcggcggaa gttttcaata ttgtgctcgg tcgtgacctt cgggtgagtc
     1981 cccgttttga tcgcatagtt ttccgccgga agcccgaagg agtcgaagcc catcgggtgg
     2041 aggacattat acccgttcat cctgaggtat cgacaataga tgtcggtggc ggtgtatcct
     2101 tcggggtgtc cgacgtggag cccgcttccc gaggggtagg ggaacatgtc gagcacgtag
     2161 aggcgcttct cttcgggaac ggtgggatct tcggttacgg caaagctctg gttctcttcc
     2221 cagtacgcct gccacttctt ttcaatggcg gaaaacgggt atttgctcat gtgagcaatc
     2281 atagcgttca cgttttgttc agtcaacgct cgccgggttc ggggtgaact ccacgacctc
     2341 cactttattg cctttcatca gaaggatctg ccacgttccg agatcggtgg tgattacctc
     2401 gccttcgacg ggaatcgagc ccgcctcctg caagagatat gccgccacgg tgctgacccg
     2461 atccccttca taccacaagt ccagatcatc gaccacctgc tgaaagggag cgtccgccat
     2521 gacgacgaac gtgccgggct tgtccttgtg ctggacgacc cggtcgggga accgcgattc
     2581 atgctcgtcg aagagctcgc cgaagagctg ttcggcgaca tcctccatcg tgacgacacc
     2641 ggagaacccg ccgtactcgt cgatgacgat cgcctgctgg agcttgtcct tcttgaagag
     2701 gaagaagaga tcgtcaaggt gcatctgctc gacgacgtag atcggttttc ggctcagcga
     2761 ggcgagggtc ttgttgacga cctgtttctg ctgggcgcga aggacatccc gcaaaaggag
     2821 gattccgacg atgttctcct gattgtcctg atagatcgga atccggctga agcccgattt
     2881 gatgatcttc gggaacgctt cttcgatcgt caggtcggag gggagggtaa agacttcggt
     2941 tcggtgggtc atgatcgtcc ggacggtcgt cgacgagaag tggatcgccc tctgcatcag
     3001 gtcggattca tactggtcga ccagccccac atcctcggcg gcgtcgacga cgtgcatcac
     3061 cccttcggtg gtgatgaccg gctcctcctt ggttccgaac aggcgggtta tcgcgctgga
     3121 aaggacttgg agcagccatg ccaagggaaa gagcagaagc atcagggctt tcatcggata
     3181 ggccatgagg atcgcgatgc gcacattgtg caccaaggca agtcgcttgg gcacgatttc
     3241 tccgaaaacg aggatcagga acgtaagaat cccggttgca tagcctacca gagtggagga
     3301 gaaatacttg atggtgattg tcgtaaccag agccgaagcg ctgaggttca cgatgttgtt
     3361 tccgatgaga accgtggtga tcaggtgatc cttgtcctgg ctcagcgcat atgccagctt
     3421 cccggcccgg gattttcgac tttccagggt cttgagctga aagaaggaaa gggaggtgta
     3481 ggcggtttcc gttgcgctga agagcgccga aagaacaaga agggcaaaaa cgacagcaag
     3541 ggagatgttc atgcacacct ccacaggcaa gatctaggta acgggtcttc cataatgggc
     3601 tcagtatatc aactcaaggc gggtgaggac aaggtaaaag aagggatccg atcaagaagc
     3661 ggacagggat ggtgggggtg gattaacaac caagtgtaca accctccgaa atattcatat
     3721 tctatcattg ctaagtatgg ttactaagta aatggaaagg agccatgacc atgaaggaca
     3781 acaatacggt aatctgttct ttttcaatgg acagaaacct ttacaatgct tataagaaca
     3841 ttgtcactca aaatggggaa aacgtaaaag acaatctcgt acggtatatg caacaggtgg
     3901 ttgattatga aataccaaat gcggaaacca tcaaggcgct tgaagaagta aagagattga
     3961 aagcggatcc ccataaaaaa acgtatggtt cttttgcgga gatcatggaa gaactttacg
     4021 atgcctaact acaggatcgt tcaaaccgga gtatgtaaaa aggatctcaa aaaagccatc
     4081 gggagagcct ttgccgaaaa aatacaggga tcacggctta tccggaaatg ataaaggatg
     4141 tagagaatgc catatagccc cggactggct tctcatctat gagatatcag gcagtgaatt
     4201 gattttatac ttgactcgaa cgggcacaca cagcgatctg ttttagcggt aacccatgat
     4261 tatgcaataa acccatgtat acgactcttt tgccaacccc ggacctttct gctatactgg
     4321 ggccatgggt gtagcattat atgatgaatc aaaaatcctg acgctcagtg cgcttgagca
     4381 tattcggatg cgtccgggaa tgtatatcgg acgcttggga aacggatcac acatcgacga
     4441 cggaatctat atcctcatca aggaagtcat cgacaactcg atcgacgagt tcatcatggg
     4501 acatggctcg aagatcgtga tccgcctcaa ggacaaccag gtgaggattc gcgactgggg
     4561 tcgggggatt cccctgggca aggtgatcga gtgcgtctcc gagatcaaca ccggagccaa
     4621 gtacaacgac gatgtcttcc agttctccgt cggactgaac ggagtcggaa ccaaggcggt
     4681 caacgcgctc tcctccgact tcgaagtcac cagcttccgg gacggaaagt tcaccaacgc
     4741 ggtcttcaaa cagggaatcc ttgtcaagga gacttccggc aaaagcgatg aggccaatgg
     4801 gacggaagtg atcttcaccc cggatgaaga gcttttcggc gattatgcat tcaatgagga
     4861 tttcatcatc gcccggatct ggggttacgc ctacctgaac accggcctga gtctggagtt
     4921 caacggccgg acgttccgct cctcaaaagg cctgggcgac cttctggcga aagaggtcgc
     4981 cgatgagggc ctctatacca cgatccatca caagggcgaa cgcctggagt tcgccatgac
     5041 ccacgtcagc ggaacctacg gggaaagcta tttctcctac gtcaacggtc agcataccag
     5101 tgacggggga acccaccttt cggcattcaa ggaaggaatc gggaagggga tcagcgagca
     5161 cttcaagaag aactggtcgc ctcaggatgt ccgtgaaggg atcatcggag cgatcgcggt
     5221 aaagatcaaa agcccgattt tcgagtcgca gacgaagaac aagctttcca acacggagat
     5281 ccgcatctgg atcgtcaacg aagtgaaaga cgcgatcatc gatttccttc tgaagaaccc
     5341 caaggatgcc gagaagatca gcagaaaggt gatgcagaac gaagcgctca gaaaagagct
     5401 gaacgaagtg aagaaagggg cccgggagtc cgcccggaaa gtctcgatca acatccccaa
     5461 gctgaaggac tgcaagtatc acctgggcca aagcgccgcc cacaaggaga agtgcgacaa
     5521 ctcgatgatc ttcatcaccg aaggcgactc ggccagcgga acgatcacca agacccgcga
     5581 tgttttgact caggcggtgt tcagcctccg gggaaaaatc ctcaacgtcc acggaaagaa
     5641 gaagaccgag atctacaaga acgccgaact gtacaatctg atggtcgccc tcggcatcga
     5701 gaacgggatc gaggatcttc gctacgggaa ggtcatcatc gccaccgacg ccgacacgga
     5761 cgggtaccac attcgcaacc tgttgatgac ctatttcctc acgtactttg aagacctcgt
     5821 tctttcaggg cgcctgtata ttctggagac cccgctgtac cgggttcgaa accggacgac
     5881 gaccgtctac gcgtacaatg agaagcagcg cgacgatgcg gtggcccggc ttcggggcgc
     5941 cgaagtgacc cgtttcaagg gccttggcga gatcgatccc cgggagttcg gccagttcat
     6001 cggcgaggat atgcgcctca tccgggtaag tcttcccggg gtgacggaga tgcaccgcag
     6061 ccttgagttc tacatgggta gcaatacccc tgaacgccgc tccttcatca tggagaatct
     6121 gatctgatga ctcaggcgca tcaacttttc cgccaaaact ttttagagta cgcctcctac
     6181 gtcatcaagg aacgggcgat ccccgatctg gtggacggct tcaaaccggt tcaacgacga
     6241 atcatccaca ccctcttcga gatggatgac ggcaaattcc acaaagtcgc caacacggtg
     6301 ggatgggcga tgcgcttcca tccccacggc gatgcctcga tctatgaagc gctggtgaac
     6361 cttgccaaca acgatctgtt catcgaccgg cagggaaact tcgggaacat cctcaccggc
     6421 gacagcgccg ccgccccccg gtatatcgaa tgccggctcc tgccctttgc gaaagaggtt
     6481 ctctacaacc ccgagttgac ggagtgggtc gaatcctatg acgggagaaa caaggagccg
     6541 gtggtgttcc ccgccaagat tcccgtcgtc ctcattcaag gagtcatggg gatcgccgtc
     6601 gggatgtcca ccttgattct gccccacaac cgctttgaag tcctcgatgc gatggccgcc
     6661 cacttacggg gtgaatcctt tgagcttttc ccggacttcc ctcaaggggg aatcgtcgac
     6721 gtcgaagact acaacgacgg gaaggggacg gttctggttc gggcgcggct caatacaagc
     6781 gacccgaagc ggatcatcgt cgaagagctg ccctatggaa cgaccagtga atcgctgatc
     6841 aattcgatcg aggacgcggc gaagaaaggc cgcctgaaga tcagttcgat caacgatttc
     6901 accgccgaga gcgtgaacat cgagatcaac ctcgcccgga acacctattc caaggacatc
     6961 gtcgacgccc tctacgccta tacggattgt gaaaaacgga tcaccaccaa cccgctggtg
     7021 atcagggaca acgtccccac ggtcatgggg gtcagcgaaa tcctcgcctt ccacgccgag
     7081 cacctcaaag tggtgctgaa ggcggagctg gagctggagg aggagaagct gcttgcaaag
     7141 atgcacgacc gcacgctgga gcggatcttc atcgaagagc ggatttacaa gcggattgaa
     7201 acgaaaagaa gcgcggacga tgtcctcaag gcggttcatg ccggcttgaa accgttttcc
     7261 agtgaactgc tccgtgatgt taccgacgag gatgtcgaac ggcttttgaa aatcccgatc
     7321 cggaggatca gcctcttcga catcgagcgg aaccgtcagg agatcgagca gatcaatgaa
     7381 cagttggagg aggttcgctc gaagctcgag aacctcaccg gctatgcctt ggagacgatc
     7441 gatcgcctga aggggatgtt cgacaggaaa gagcacaaga gacgatcgac gctgaagaag
     7501 ttcgagctgg tggatgtgaa ggaggtcgcc gtccgcgacc tcgttgtccg atacgattcg
     7561 accaacgggt atctgggcta cggggtgaag acgggagccg agcaggtgac ggtcagccaa
     7621 ttcgaccgcc ttctggtgat ccgccgcgac ggttcctatc aggtgatcga cgccccggag
     7681 aaggagttcg tgggcaaggg gatgctccac tgcatgatcg ccgaccgtga cgagctttcc
     7741 aagatcgtct tcaccctgat ctatcaggag aagacctaca aatacacgtt catcaaacga
     7801 acccaaatca cctcgttcca gctgaagaag ctctatccgc ttcttcccga cgagaaaaac
     7861 tatcgggtcg ttcgcctgtt gacccatccg aatgcggaga tcggcgtaac gtacaaaccg
     7921 aaatcaggac tgcggattct ggaggagaag ttctacctct ccgatttcct ggtgaagaac
     7981 ccccgggcga aaggggtgcg gatgaccgtc aaggagatcg cttcgatgcg gatccgatcg
     8041 gtgaaagggg atgtttcttc ggcgaaagat cccgaactct tcgatgaaga cgaggaagaa
     8101 gcgtgagatc cttttccgag cgaatcctct atgaggacaa ccatcttttg atcgtgaaca
     8161 agcgctgcgg cgaattggct caaagggatc aaagcggcga tcccaccctc ggggattttg
     8221 ccaaggaata cctgcgggtt acctacgaca agccggggaa catctacctc ggcattcccc
     8281 atcgcctgga tcggccgacc agcggggcgg tggtgcttgc aaagacggag aaggccctga
     8341 tccggatgaa caccctcttc cgggaggaca aggttgacaa gacctattgg gcgatcgtcg
     8401 acaaggctcc gccggagatc gaaggcacgc tggtcgacaa cctcgtcaag gatgccaaga
     8461 ccaacaagag ccgggctttt cccgccgact cgaaaaaagg aaagcgagcg gttctttcct
     8521 atcgccaccg agcgtccagc aagaacttcc accttcttga aatcaccttg gaaacgggaa
     8581 ggcaccatca gatcagagcc cagcttgcag cccacggtat tcatatccga ggggatctga
     8641 agtacggcta cccccgttca aatcgcgacg gagggatctc cctccacgcc cgaacccttt
     8701 ccttcatcca ccccgtcaaa caggaacgga tcacgatcgt cgctccggtt cccgacgatc
     8761 cgctgtggaa ggtgtttgaa gagatgacca cgtgaaaaac ccctgccttc ttcgtgcagg
     8821 gggaagtcga ttaataccaa ttccaacatt gcggtttttc cggaaccgct ttatctggcc
     8881 ccatgatacc atgagttttg cgtgaggcaa agggaaatta tcataattct gacgcttttg
     8941 atgaaaaatg acgccttctc tgagcgccag gtgaaaagtt gacgcttacg cagtgcgggt
     9001 tcctatggga gacttacacc atggaaccac ctaccattag acaaaaaatg gccaagcact
     9061 ggcagttcta cctcatcgtc tccctgccgg ttctctactt ccttctcttc aagtacctgc
     9121 cgatgtacgg catccttctg gcgttcaagc gctaccgggt gagcatgggg atctggagaa
     9181 gtccttgggc cggtctctat cagttcaaga agttcttcaa caatccttcc gctctccggg
     9241 tgatgtacaa cacgctcgtg ctgtccgtct acgccctcgt cacctcgatt ccgctggcgg
     9301 tcatcctcgc gatcgcgctg aatgagaccg gcagcaagct gtggcggaag acggttcaga
     9361 tggtgaccta tgccccgtac ttcatctcca cagtggtcat ggtcgcaatc ctgatgcagt
     9421 tcctcgatcc cgcgctgggg atgttcaaca cgatccgcaa tctcttcggg aaggaatcgg
     9481 tgaacttcat gggcgaggcg aagctcttca agcacatcta cgtctggtcg gcgctgtggc
     9541 agaataccgg ctacaacgcg atcatctatc ttgccgcctt gaccggaatc aaccccgaac
     9601 tctatgaagc ggcccgggtg gacggggtga acaagttcca gaagatctgg cacgtggatc
     9661 tgccctcgat caagacgacg atcgtcatca tgttcatcat gaacatggga ttcatcatga
     9721 gcctcgggtt cgagaaggcg tttctgatgc agaatcccct caatctggag agcgccgaga
     9781 tcatgtccac ctacgtctac aagatgggtc tgatcaacag cgacttctcc tactcgaccg
     9841 cgatcgacat gatcaacagc gtgatcaaca tcatcctcat ccttaccttc aacaagatcg
     9901 ccaagatgcg gaatgaagga ggaggtctct ggtaatgaaa aaacatcttg cttccgacag
     9961 gatcttcgac ctgctggtaa acatcttcct cgtgttcagc ttcctcatcg tcctctatcc
    10021 cttgctgtat gtcgtctcct cctccctgag ccaccccacc gccgtgatgg ccaacaaggt
    10081 gtggctgcta ccggtggact tcgacctgat gagctacaag gcggtgttca cgaacaagca
    10141 gatcggaacg ggctatctca acagcctcta ttacatggtc gtgggaacgg tcgtgtcggt
    10201 tctgttgacg atgctgctcg cctatccgct ttcccgaaaa gagttctatg gccgttcggt
    10261 cgttacgaag ttcatcctct tcacgatgct cttcagcggg gggacgatcc ccctttatct
    10321 ggtggttcgg aacctcggcc tctatgacag caggtggtcg atcatcctgc ccaacgctat
    10381 taccgtctgg aacgtgatca tcgcccggac gttcctgcag gagaacatca ccgacgaact
    10441 ctacgaagcg gcccagatcg acgggtgcag cgacatccgc ttcctcatct ccttcgtctt
    10501 cccgctcagc ggagcgatcg tggcggtcct tggactcttc tatgcggtgg ggcagtggaa
    10561 caaatacttc gacgcccttc tgtacctgca gaaccaggcg ctctatcctt tgcagatcgt
    10621 cctgcgcaac attctcatca tcaaccgcaa caccccgtcg atgaccgtcg acgtggaggc
    10681 cgcgattcgc gcccagggcc tcagcgaaac gatccgctat gcggtcatcg tggtcgccag
    10741 cgcccccttg ttgctgatct atcccttcgt ccagcgcttc ttcgtcaagg gcgtgatgat
    10801 cggatcggtc aaaggttagg aaatccccgg accatcggtc cggtgaacac attaggagga
    10861 atcaaatgag aaaatcgatt ctaatcgtac tcttcgcact gttgctgccc gccctgctgt
    10921 ttgcccaggg gtcgaaacgg gaaccggaga agccggcggt cgaatacacc cccatcggga
    10981 cgttcccgat cgtcaaggag aagatcaccg tcgacatcat ggtcgtccag cccccgtgcg
    11041 tcgaggatta caacaccaac cgcttttcca agtacatgga ggagctgacg ggaatccacg
    11101 tgaatttcat catggttccc gagcaggccc ggcaggagaa gcttgccctg gtcctggcca
    11161 gcggcgacta ccccgacgca ttccttggat tcggaatcgg aacggaactg gaaacgacgt
    11221 atggctccca ggaaggcctg ttcctgcccc tgaacaaata ctactccaaa gagtggatgc
    11281 ccaacatgat gaaggcgttt gaagagttcc ccggcggtat cggatatatg accaacatcg
    11341 acggaaacat ctactcgatt ccgcgcctcg aaggttgcta ccactgttcg aacatggcga
    11401 agatgttcgt ctatcagccc ttcctggacg cgctcggact gaaggtcccg accaccaccg
    11461 acgagttcta ccggacgttg aaggcgatca aggagcggga tccgaacggc aatggccgag
    11521 cggacgagat ccctcttgcc ggttcgatca tcggctgggg cgatcaggtc gaacgcttcc
    11581 tgctcaacag cttcatctac tgtgacctgg acaccaacat caacgccaac gccgaaagca
    11641 acgtgggttt catgatggtc gacggaaagg tcgacacggc gatgaacaag gacggatatc
    11701 gcgaaggcct tgcgtatgtg cacaagctct acaaggaagg actcatctac agcggatcct
    11761 tcactcagga ttccagccag ctcacccagc tcgtcgaatc gtccgcccag ccgacggtcg
    11821 gcttcgcgac cggcggatgg agagggcagt tcgccacgat cggaggcgac cgcttcaaca
    11881 acttcaaggc gatcgctccc ctgaaggggc cgaagggcgt ccaatactcc gtggtgttct
    11941 ttcaggatcc ctcaatgggc agcctggttc tctcttccaa gaccccctat gccgatgcga
    12001 tcatgcgcta tttcgacttt atgtacactc aggaaggcac gctcatgcag cgcaacggct
    12061 tccagggcga agcatgggat tgggcgaaac ccggtcaggt cggactcgac ggcaaacctg
    12121 cggtctggga gcagctgaag ctttggaacg acaaggatcc ccagaacgac acctggatcc
    12181 agacctacgc ctcggcgacc actccgagcc tgaagaacgg cctggcctcg ccgccgatca
    12241 ccccggagga tcccgagtac tatctgccga gcaacaacga gaagaccctc ttcgacgaga
    12301 cgacctatct gtacaagcca tacgaagacg tcaccgtcga agttcctcac ctgaagtaca
    12361 ccgccgagga gaacgagcag ttctcgaccg tcaagcgcga gctcgccaac tacatccgcc
    12421 agagcgccgt gaaattcatg gtcggttcga tggatgtgca caacgaccgc gtgtggaacg
    12481 actatgtggc gggtcttgaa aggctgcaga tgagcaacgt ccttgcgttg atgcagaagg
    12541 cgtacgatcg tcagtacaag taggtccgta tgagtggggt cgacaaaccg aacgtcgtct
    12601 atctgttgtt tgacgatctg ggatatggag acgtttccgc cttcaatccg aaggcggggt
    12661 tcaagactcc acggatcgac cggctcgcca agatggggat gtgcttcacc gacgctcacg
    12721 cgacgagcgc ggtgtgcacc cccagccgct attcgatcat cacggggcgc tataactggc
    12781 gctccaccct gaaatcctcc gtcctgggcg gctattcaaa gccgctgatc gcgcctgcga
    12841 gacggacgat cgcgaacctc ttcagggata tcggataccg aacccatgcg gtggggaaat
    12901 ggcatctcgg cctggatctt cccaccggtg aagggttcgt cgaagccccc gggttcgccc
    12961 agtccggccc cttggagcat gacaggccga tcggcggagg gccgaccgcc ctgggcttcg
    13021 acacgttcta cgggatcagc gcatccttgg acatgccgcc gtacctctac atccggaacg
    13081 accggttcac gagcgccccg acgcggacga cgagaagcga cggaatgggg tattggcggg
    13141 aagggccgac gggggaggat ttcatccacg aagaggttct cgaccatctt accggcgaga
    13201 cgctgagaat catctcctcc ggcgatgaaa gaccgttctt cctctactac gcgctgaccg
    13261 cgccccatac cccgatcctt ccggacaggg agttccgcgg acgatcgggc accaacgcct
    13321 acggcgactt cgtcctgcac tgtgacgatg tggtcggacg gattctcgat gcggtcgacg
    13381 agaacaccgt ggtggttctc accagcgaca acggatgctc gcctcaggcg gactttgagg
    13441 agctgaggag gttcgggcac catccaagcc atcatttccg gggaatgaaa tcggacatct
    13501 atgaaggagg gcatcgggtt ccctacatca tcgtatggcc gggtgtcacc cgggccgata
    13561 ccgtctgcga ccggacggtg agcctcgtcg acctctatgc gaccttcgcc gaatacttcg
    13621 gactatccct cgcctccgat gaggcggaag attcggtcag catgctgaat ctgcttgaag
    13681 accccgacag cgaagcgaag cgaacctccc tcatccatca atcgatcgac ggatcgcttt
    13741 cgatccgcaa aggtccgtgg aagctcgagc tgtgtcccgg ttcgggcgga tggtcgcccc
    13801 cggttcccgg cagcgccgag gagaagctcc ttccggcgat gcagctctac aaccttgatg
    13861 aggacatcgg ggagacgaca aacctcatcg accggcatcc cgaggttgtc cggcggatga
    13921 aggcggagct gctgacgatc gtccgtgacg gccggtcgac acccggggcg aatcagccaa
    13981 acgagggagc cgccttctgg gaaacggcgg cgtatctccg accgtaacct aaccgagggc
    14041 tccgaaccct tcgcgaagca tccctttgta gtctccgtgt cccttttttc catcgtcatc
    14101 ctgttgtatg gtatagtttc tcccagtgag gaacacttga tgacgacacg ccgacccgcc
    14161 cttcatttct cagctcggaa aggatggatc aacgatccca atggtttctg cttcttcggc
    14221 gggaggtatc acatcttcta tcagcacaac ccttatgcgc cgcagtgggg gcagatgcat
    14281 tgggggcatg cggtcagcga cgatctggtc ttctggaagg agcttccgat cgcccttgtt
    14341 cccgacaggt ggtatgagga cgatcccaag ggagggtgtt tctccggatc ggcgatcgtc
    14401 atcgacggca ttttgcatct atactacacc gccgcggccg gcggacacca gaggcagtgc
    14461 gtggcgacga gcagcgacgg ggtgaccttc gtcaaagcgg agcggaaccc gatccttgaa
    14521 gctccttcgg ggtttgagga tttccgcgat ccgaaggtct tttttatcga agaacgcctc
    14581 ttcatggtca tcggggcgtc cgacgggacg gagcacgtgg tgctactcta tgagggatcg
    14641 gatccctata cgtgggagga gcggggagtc ctctattccg ttccccggga aatcgggacg
    14701 atgccggagt gtcccgactt cttcccgctt gacgacaaat gggtgcttgt gttctccccg
    14761 atcggaaagg acacccacgg cgtcgtcgca gtcatcggga cgttcgatgt acatacctgt
    14821 tcttttacaa gcgaatcgat ccagccgctg gatcacggtt cgcactatta cgcgttccaa
    14881 tcgacttcgg gaaacgaccg aaagcggtat gcgttcgcct gggcggggca gtggccgtgg
    14941 atggggcgct tttccggcca cggatcggat gcagaagggt ggcgggggtt tctgaacttt
    15001 cccagaagac tccggtacgt cgacgggcgg atccattcat acccggtcga agggatcagg
    15061 gatctgttta ccgattcgtt gatcgacaaa tccctgaagc cgaactgcga tctttctctc
    15121 acttctcttg gtggcgtgta tctgatgagc attacctata gctcttcctt ctcgcttcgc
    15181 ttcggatcga cgcagctcgc cgttgattcc gtcagccata cggcgacgtt cacccatcac
    15241 gggacgacgg gcatcgctcc gcttgcggga ggattggttg aaatcttcgt cgaccggatg
    15301 agcacggaga tcttttttga cggcggatat gcttcgatct gcttcaacag gcatgaggac
    15361 gaggatgaca ccatgatgat ctccaacggg aatgccccgc tgaagcttag cgcgtcagga
    15421 tcaccccttc accgctaaac ccgttcactt tgacaatgag cggcgcccgg gcatggatga
    15481 agtgcttcgt ctccttgatg accggcagct cccgcaggcg cctgacgttc aatgttccct
    15541 ggccggactc cgggttgatt gtcaggtaga acgagcccag actggtgagg ttgtggaaaa
    15601 agtgggttcc ctggctcggc tcggccctca gatccttcaa gccagtctcg acgatcacct
    15661 tgcttcgtga gatctgggac caggaaacgg ggatcccgag ccagggatcg gaggaaccca
    15721 gccggccggc gaccacgagg acgtaatcgc gttcggaaag aaccatgccc gtgttcagct
    15781 catccagttc attggccatc tcgaccatct tggagggatc gaaggcatca agcttgacgc
    15841 agatgagatc ctcgatcgaa tcaagggttc cgttgcccag cacggaggtg gaaaggatca
    15901 atgcggcttt gcggtcatcg tcttcgatcg agacatcgat ttcttccgaa ccctgggcga
    15961 tcgggcggat ctgcagaagg ctgaactccg gcttcttcgg agctttccgg ttgaggttga
    16021 ccgcaaactc gatttcaacg ggtctcccca tcgcctccga gccgacacga agcacatccc
    16081 ggaccaccgg ggcaaggggg aatgcatcat acttcaacac gccgttgaag gtgatgatct
    16141 tctttcccac ggacccagcc gcttcattga acgtctggtt ggcgaagtcg aaggtggaag
    16201 cgacatatcg aaggctttca gggtgggctt ccgcttcact cagatcgacc agcttgaggt
    16261 tgtccaatcg gtcgagccgg ggaacgaacc cttcacggag gtcgagggcg tagaaccgct
    16321 cttggctgga ggattgattt cccccaagga attgagaagg ccgtttcggg tgggtggggc
    16381 tgaagcgcag ggcgctaccg ctttccacca ccgccttgcc gaacccgaag gagagcagtc
    16441 cgacgccgtc ctccggcttt tcaccgcttg gcggatagta gttgatcgaa cgggcgacgc
    16501 cgccgatgtt gggataccaa tacgagccgt gttgactacc gattacctgc tggatgatga
    16561 tcgccatctt ctcatccccg agaaggtagt cggtcgcctt cacatactcc ttcgcccggc
    16621 ggaagtaggt ggacgcccac accgatttga ccgcgttgga gagttcctcc aacctgagct
    16681 cttcgcttcc ccgattggga atcatcaccg tttcatacac tccggcaaag ggctggtagt
    16741 gggaatcctc caacaacgag gaggagcgga cgcagatcgg ttgtttgatg acggagagga
    16801 tatgtttgag gttctcgatt agatcttcgg cgaggggttt tgagagaaag atcgaaagca
    16861 gcgtatcgtc cggcaagtcg gcggtggtga tatccttcaa gtcattcgct tcgatgaacg
    16921 attcaaattg atcggtggcg atgacgacgg atcggggaat tgaaagatag atatccgggt
    16981 atttcgccgg aatgcccgct tggcgcagga ccatgtcgat gaacgccaag ccgcggcctt
    17041 tgccgcccag cgatccggtt ccgatccggc tgaagaagag ggtctcgtcg tagttcgccg
    17101 gtgagaactg ggcgatgact cccttggttc gctcggcgcg gtagcttcgg atcgtttcga
    17161 tcatctcgtc gcggatgagc gccacgtcac cgccttcggg aatcttcagc gccttgattt
    17221 tcgcggcgat cgtatagagg ctctgcgctt tcagccagcg ggagaagtgt tcattgcgga
    17281 agtggtattc gaaggaggcc agatcgatct ccggaaggat atgctgcagg gcgcgcatcg
    17341 tctccacccg ggcgatctcc gccccggtcg cgggattccg gaagatgaag gggccgaagc
    17401 cgtagttggt tcgcatatag gaggcgagct gggtgaagag ggagctgctc cgcttgtaga
    17461 tgaaatgcgt tccggtcttc tctgccgcct ccttcatcga aagatcgttg gattggagga
    17521 ggatcggaag ttcgccgttt cggctatgga gctccttggc gagggcgaag cccgcatgct
    17581 tgttgtgaac cccctccatc tcgtaggaga cgtcggtgat gactcccaag acgtgttgtt
    17641 gatacttctc gtacagggcg agtgcttcct catagttttt ggccaggacg atcttgggcc
    17701 ggccgcgcgt cctcagcgtt tttccccagg tgttcagcgc ttcaaggatc atcgcctggg
    17761 tctgctggat caggcacgtg tagatcatcg gcaggtaaga ggagtagaag cgaaccgagt
    17821 cttcgacgag gatgatcagg ccgacgtccg cttcggcgat atcgtggtcg aggttcatcc
    17881 gatcctcaac cagcttgatc atcgccagaa agatcgtggg atcgccctgc cagtagaaga
    17941 agtagtcgat cgcgatcgaa tcgctgccct tgatgagctt gttccgtcga tggccggagg
    18001 agggggagag ggcgatgatc ggcatcccgg gatccacctg cttgatggaa gaggcgaggt
    18061 tctccaccga ttccgggccg agatcgagca tcgtgatgac cagctcgaac gtccgggtct
    18121 caagcagctc aagcgcttcc tcccctccgg aggcgtaggt gatcttcggc gggttggaaa
    18181 ggcgcagctg ggtatactcg atgtacagtt cctcctctac ccgcccgtcc tcctcaagga
    18241 ggaagcggtc atagtcgttg cagatgagga gcacattgta gatgtggttg agcatcaggt
    18301 tgctgaacgg cagccaggtc gggtcgaggg tgtacatgaa aaaagtgtag cacgggcgca
    18361 taactttgca taggttcatt aaattactgc atattttata tatacgcaaa atataggttg
    18421 aataattttt gaattcctaa aatcagatag gctaaatttc aacttaggcg aattggcctc
    18481 ctttacaaac caaaaagttt tgtctattgt gctacccaac aggagcgaga tgtatgtata
    18541 acctttcaga ttttatgaat gagctcgaac gcaggaaccc cgcccaaccg gagttcatcc
    18601 aagcgacccg tgaagtgctt gagtcgatca tcgacgcggt gaacgggaat cccgtctatg
    18661 agaagcatcg aatcctcgag cgcctgaccg aggcggatcg catcttctcg ttccgagtcg
    18721 aatgggagga tgatgagggg aatcttcaag tgaaccgcgg ataccgggtt cagttcaaca
    18781 acgccctcgg cccctataag ggaggactgc gctttcatgc aagcgtcacc ctcggaaccc
    18841 ttaaattcct cgccttcgag cagaccctta aaaacagtct gacgacgctg ccgatgggtg
    18901 ggggaaaagg gggaagcgac ttcagcccca aagggaaaag cgacggggag atcatgcggt
    18961 tctgccgctc ctacatgagc gagctccaga agtacatcgg tcccgatacc gacgttcccg
    19021 ccggagacat cggcgtggga gcgcgggaga tcggatacct ctacggacag tacaagcgcc
    19081 tgcgggacga gaacaccgga gtcctcaccg gcaagggcct gaactacggc ggatcgatcc
    19141 tccggccaga agccaccggg ttcggcgcga tgtacttcgc caactcgatg ctcgcgctca
    19201 gaggagacag tctggaaggg aaaaccgtcg ccctttccgg attcggaaac gtcgcctggg
    19261 gcgccgccca gaaggcgacc cacctcgggg cgaaggtggt tacgatcagc ggaccggacg
    19321 ggtacatcta cgatgaagag ggggtgggaa ccccggagaa atgggcgtac atgtcctacc
    19381 tccgcctttc caacaacgat gtggtcgagc cgtatgcgaa agagttcggc gccgtgttcg
    19441 tcccccggaa aaagccgtgg gaggtcaaag tcgattttgc gttcccctgc gcgatccaga
    19501 acgagctcag cctggccgat gcgaaagccc tcgtggccaa cggagtccag ggggtcgtcg
    19561 agatttcaaa catgggttgt caggcggatg cgatgtacta tttccttgaa aagaagatcc
    19621 tcttcgctcc cggcaaggcg gccaacgccg gcggagtcgc cgtcagcgga ctggagatga
    19681 cacagaacgc gatgcatatc gcctggaccg cccaggaggt ggatgagaag cttaaggaaa
    19741 tcatgttgaa aatccacaac gcctgcgtca aggaagggac gcaaccggat ggatacatca
    19801 actatgtgaa gggtgcaaac atcaccggct tcagaaaagt ggccgatacg atggtccagc
    19861 tcggctatta accttgtcca actttgtaca ttttccggcg accttttgga ggtcgtcttt
    19921 tttcatgaca ctagcggccg accggaagca gagatttcgt catcggttga atcggtattt
    19981 tccgaaaata cccgatattt ccgcttcctg cacacagtgt gggaacctcc tgccggcggg
    20041 gaatcatcct gctttgtaag gaattacaca ccgggtagta tggggttggt atccaactgc
    20101 tacaaaactt atataacact atatatagtg ttgtaagaag ttagtaacac tatttatgcc
    20161 gtatttgtgc ttgcaaatcg gtaggcgggg tgctatgctt gccatgggcg gtcaccgccc
    20221 cttcccgtta tttgaataca taaatacgat atatgaggat aagcatgaat gccggcaaga
    20281 atgaattaac cgctttgggc cgaaagatct ttctggatcg atacgccctg aaagatgtca
    20341 agaaggaaac gctcgccgtc ggcgatatcg tcgtcgcggt cagcaacccc cagaccgggc
    20401 agcgggagat cgggacggtc accgccatca gggacggaga cgagatcgtc gtcaccttgg
    20461 atgacggggt ggttctggaa gtaaagcggg aagagatcga taaaccgatc gagaccgagc
    20521 cgggccagat gctgaaccgc gtcgccaaag ggatcgccgc tcaggagaag aaagagatcc
    20581 gaagcaagtg ggagaaggag ttcaactgga tcttggagga ttggcgcttc gttccggggg
    20641 gaaggattct caccggggcg ggaaccgatc agaacctgac atacttcaac tgctatgtga
    20701 ttccctcgcc caaggacagc cggggcggga tcatgaccag cctggggcag atgaccgaga
    20761 tcatgagccg cggcggcggc gtggggatga acctctcctc cctgcgcccc cggcacgcct
    20821 acgtcaaagg ggtgaacggc cgctccagcg gatcggtcag ctggggcgga ctcttcagct
    20881 tcgtaaccgg cctgatcgag cagggcggat cccggcgggg ggcgctgatg ctgattctca
    20941 acgtctggca tccggacatc atggatttca tcaactccaa gcgggagatg ggcaagatca
    21001 ccaacgcaaa catcagcgtc ggcatcaccg acgagttcat ggcggcggtt cgttccgaca
    21061 aggagtggaa caccgtcttc ccggatacct ccgacccgaa atacaacagc gattgggatg
    21121 gcgacttgga ggcctggatt aagaaaggcg gctccacggt cgtctatcag acgctcaaag
    21181 cgaaagaggt ctggaacgcg atcgtcgaga gcgcctgggc gagcgccgaa ccgggggtgt
    21241 tcttcatgga tcggtacaat cagatgagca actcctggta ctactcctcg atccagtcca
    21301 ccaacccctg cggcgaacag ggcctcccgc cgtggggggt gtgtaacctc ggctcaatca
    21361 acctcagcca attcgtcaag gacaagaggg ttctgtggga tgatctcggg cggacggtcc
    21421 gctcaagcgt ccgctttctg gacaacgtca tcgacgacac gccgtacttc ttcgaagaga
    21481 acaggaagca gcagcagggc gagcggagga tcgggctcgg gacgatgggc cttgcggaca
    21541 tgctgatcaa gatggagctc gcctacggaa gcgagccctc cctgaagttc atcgacgaac
    21601 tctacaagtt catctgcctc gaggcgtacg acgagagctg caaccttgcc gaagagaagg
    21661 ggagcttccc cttcttcgat gcggaaaaac tgctgcaaag cggctttatg atgcagatgc
    21721 ccgaggagat ccgggaaaaa gtccggaccc atggcctgag gaacgtcacc ctcctgaccc
    21781 aagcgccgac gggaacgacc gggacgatgg tcggcacctc gacggggatc gagccctact
    21841 acttctggga gtgggagcgg accggccgga tgggaaccaa tatcgagcgg gtcaaggtgt
    21901 acgaagactg ggtcaaggcg aaccccggca aggaaaaacc cgattacttc gtcagcgcga
    21961 tggatctcgc tccggagggg cacgtctatg tccaggcggc gattcagcgc tgggtcgact
    22021 cctcgatctc caagacgggg aacactccca aggagtacac cgtcgagcag accggaaagc
    22081 tctacgagct gctctacgac acggggtgca aggggggaac tacctaccgc gacggctccc
    22141 gggacacgca ggttctgacg atcaagaagg atgaggaaga agagaagaag gagacggtgc
    22201 taagcttcac cgatccgaag ccgcgggttc gggcgacggt ccttcgggga accacctacc
    22261 gcaaggcgac gccgatcgga acggcgtaca tcaccgtcaa ctgcgacggt actgatccga
    22321 gcgacatctt cgaggtgttc atcaacgtcg ccaaagtcgg ctccgacgtc gccgccgacg
    22381 ccgaaggcct cggccgcctg atcagcctca tccttcggat gccctcgccg ctcacccccg
    22441 aacagcgggc ccagacgatc atcacccagc tacagggaat cggcagcggc cgttcgatgg
    22501 gcttcgggaa aaatcgggtg atgagtcttc ccgatgcggt agcccaggtg cttgaggagc
    22561 acctcggctc caccccgagc gagcgtcctc caatgaatct gcccgatgaa gaggacgaag
    22621 aggagggcga aggccagctc tttttgagcc tgtcctcctc caagccgggt tcgatccagg
    22681 cggatatctg cccggtgtgc ggcaacggga cgttcatgaa cattgaagga tgccggaagt
    22741 gcttctcctg cgggcacagc gagtgctgag aagataccaa gaagggatgg gatagggggt
    22801 ccgctcaggc gggcccccta tccgtcatat cagctcaagc tcggcttcaa actccccgtc
    22861 gagggggaag atcggccggg gcaggttcct gtattcgagc cgctcgaaga gctcgtcgga
    22921 ggatccggtg gttagggcca tcattgaacg tcgggcgatg gcgcggatct ccggctccag
    22981 atatccaagc ttcacgacga tcaccttccg gttttcgacc tccaccccca acgcccgcat
    23041 catctcgggg tcgtagcatc cgacgtgctt cgtcatcgcc accatgtcga tccccccgat
    23101 gtgcagcagg gcgagccggg ttccatgctc ccaccggttg aacagcgcct tgacggtgaa
    23161 cgatccggcc accggggagg atttcgccgg gtcgaagcga ctgccgatcc ggctctcgat
    23221 cacttccccg actccgacct tttccgcctc cgcgacgatt tgcgggtcgt agaaaccctg
    23281 gtagatcagc ggcggatcga gccgtgaaag ctccggatcc ttcaagagga ggtgcaaaaa
    23341 gccggtcacg tccccgctac tgcccgcggt gggattgtcg ccgctgtcgc tgatcaccac
    23401 gggatggatc ccttctgcca gcgaccgttt cgtcgccgcc atcgcctcct cgggcaggtg
    23461 ggtctcggtg tagaaggtga actcgttgcg cttcgcccag aacaggtcgg caagctcccg
    23521 cgccagttca tcggcgaggg cctgattctc ttccgtgacg accaccgcat ggactccgtt
    23581 ctccgtgccg tccgcccagg gaaagcccag cagataggag caggcgagga ccgaggagcg
    23641 gcgttcccgc ctccgaagct ccccgatcag ggagcgcatc ggctcgacgc cggtttcgct
    23701 ctgctcgccg gcaatgagca tcgggatgcg caccgccacc atcgtgggcc gcttcttcga
    23761 ctccagcgtc cggatcgtca tcagggcggc atggattccc gtctcatagg tgtcggtgtg
    23821 cggggcgcac ttgtacccga cgaaccccga aacgaggctt ttcattctct tcgtcagcgt
    23881 ggcgtgcatg tccagcgagg cgaagatcgg gatcgacggt tcgatcgccc ggatcgagga
    23941 aaggaggtcc tgctccgctt ctccgatccc ttcgacccgc atcgatccgt ggagggggag
    24001 gcagagcgca tccagcggcc cggcgttcct caagtcggtc aaaagctcct ccttcagctc
    24061 ttcgtagcat gagcgctccc acaccccgtt gggaaccgcc cgggcgacga gggagggtac
    24121 gacctcatat ccggcggcga tgagcgtctg gatgatgccg cttgcggagc tctcccgttt
    24181 gaccagaagc tcctccctgc gcgtgacccg gatgtcctcc cgggaggtca ggatcgggtt
    24241 gaaggtatcc gactcgtggt ggagtccgcc gacaaagatt cgtcctttca ttccttgtgc
    24301 tcctttttta tcgtcatcat cagatccggg caggtcagct cggtgcgctt catcggctcg
    24361 ccgacggtgc tcacttcgag ctgatgggtc ccggattgaa ggtgaagctc ctcggcgagg
    24421 attcccgagc gctgccctgc ctggagggtc gcatgccaca ggagctcgtc gagcctgatt
    24481 tggaccttca ccgcctcttc cagcgggggt tcatgctccc agaccagcga gtaggttcca
    24541 tgctcctcga gagcgacctt ccacgccagg gtggtcggaa tcggcttgta ggtgtagtag
    24601 tccgtgcccc catagcggta gtgcacgcta ccgaacgtgg gcgttccgag cggaaggtcg
    24661 gcgctcggaa ggcgggggtc ttccgcttcg aggggctttt cccggagccg tttcccgatt
    24721 ccgaggagca cctcctcttc gaacgggacg atcgagccgt cccccttgag gccgatgttc
    24781 agaaggtagg tccctcccgc cgccctgacg gatcggagcg tctcggtcag ttccgtaacc
    24841 ttgtcttcca gatctccccg ctcctgccac gagcgatatc cccacgtctc cttgtagatc
    24901 gaagcggggg tctgccaagg acagttcagc gggagggtgg ggatctggtt gtcccccatc
    24961 gtgaggaagt cctgatagtc gttccagatc ctcccgttga cggcgcagcc cggctggagg
    25021 ctcttcacca gagcgtacac ctcggcggac tgctcggcgg tgggggcacc catgtccatc
    25081 cacagctccc ggatcgggcc atagccgcta agcagctcgg tcagctgggc gatgttcagc
    25141 ctctggtggg cgggggggat cgcatcgcag ttgtgggggg agatcgggag agcgtggggg
    25201 aagttccaat cgatccagga gaaatagaca ccgaattcca gaccgcccct ccggcatgct
    25261 tcggaaagct cccgaaccgc gtcctttccg tagggggtgt tgatcgagtt gtaggcggtg
    25321 ctcttcgtgt cgaagaggca gaatccgtcg tggtgcttcg cggtgatgac gagataccgc
    25381 atccccgccc gcttggcgac ggcgacgatg tggtcggggt cgaagcgctc gaaggtgaac
    25441 cggtcgacca gccgttcata atcccattgg ggaagatagc cgtgtgagag gatctgttcg
    25501 ctgtagccgc gaatgacttt cctgtcctga tgaaccccgc ccagaatcga gtagagcccg
    25561 acgtggatga agaggccgaa gccgagcttc cgccaagatt gtatcgttgt ctgcatgggt
    25621 caagcatatc gacatccccg atgcatagat aggggcaaag tttgaacggg gcgtcaaatt
    25681 ttgggttccg aggcgaaacg gaaggggatc cggtgatgga tggccgagct cgaattccta
    25741 ccatttttat agtgattttg ctgtttgttg gtgatgtaaa gagttagcgt ctagaaactt
    25801 tatttcttta ctccgaatag aatattttcc gtgttctctt gcaaacgaac ccttattggt
    25861 ctatgattgg ctactgggaa aaatctgcga tcttcccaca tttttgtgct tagggcagcc
    25921 ccttccgggt tgcagcaaat actgctttat ggagagtcac atgagtaaca aaaggaaggt
    25981 taccatcgac ggaaacaccg ctgctgcgca cgtagcatac gcgtttagcg aggttgccgc
    26041 catctatccg attacgccct cttccacgat gggtgagttt gccgacgaat gggcaagcgt
    26101 tgggcggaaa aatatgtggg gaaaaacggt cgaagtcgtc gagatgcagt cggaagccgg
    26161 tgccgccggc gccgttcacg gttcgctcgc ggcgggtgcc ttgacaacca cgtttaccgc
    26221 cagtcaggga ttgttgttga tgattccgaa catgcacaag atcgccgggg agatgatccc
    26281 gaccgttttc cacgtttcgg cccgatctct tgctgcccaa tccctttcga tcttcggtga
    26341 tcattccgac gtgatgagct gccgaaacac cgggtttgcg atgacgtgcg cgaacaacgt
    26401 ccaggaggtc ttggaccttg ggctggtcgc tcacctttca acgcttgagg ctcaggttcc
    26461 tttccttgcg ttctttgacg gcttccgcac ctcccacgaa gtgcagaaag tcgatgagct
    26521 tacctacgag gacatgtaca ccctcgtcga tgagaaatat gtccgccgct tccgcgaccg
    26581 tgcgatgcgc ccggagaagc cggttctccg cgtagccgct cagaacgaag acgtatactt
    26641 ccaaggtcgg gagacggtga acaaatatta cgaagccctg ccggagatcg tccagaagta
    26701 catggacaag gtcgccaagc ttaccggccg aacctatcat ctcttcgact acgtcggcgc
    26761 tcccgatgcg acggatgtgg ttgtcatcat gggaagcggt gccgacacgg tggaatgggc
    26821 aagcgactac atcaacgagc gcggcggcaa gaccggtgtg ttgaaggttc gcctctatcg
    26881 tcccttcagc gccaagcact tcgttcaggc ccttcccgct tcggtgaagc gcgtcgcggt
    26941 tctcgaccga actaaagagc cgggcagtct cggcgagccg ctctacctgg acgtcatcac
    27001 cgccctcaat cagatggatc gaaccggtat caaggtcatc ggcggccgct acggtctttc
    27061 cagtaaggac ttctccccga cccacgcgaa ggcgatcttc gatcacctcg ccggtccggc
    27121 gttcagcaac ttcaccgtcg gcatcaagga tgatgtcacc aaccgctcca tcgagatcaa
    27181 ggagcagatc aacgtcattc cgaaggatgt cgtctcctgc atgttctggg gtctcgggtc
    27241 ggacggtacc gtcggagcga acaagaactc cgtcaagatc atcggcgaca acaccgatct
    27301 gaacgctcag gcgtacttct cctacgactc caagaagagc ggcggcatca cgatcagcca
    27361 ccttcgcttc ggcaagagct cgcttgagat gccgtggctg gtcgaccacg ccgatttcgt
    27421 cgcctgtcac aatccggcgt atatcggccg ctacgacatg ctcgctccgc ttcgcaaggg
    27481 tggcgtgttc ctcctaaaca gtcagattcc ttccgatgag gtcttcgacc atctgacccg
    27541 cgagatgcag gagcagatta tcgaaaagga aattcggttc ttcaacatca acgcacttga
    27601 aatcgccgaa ggcgtcggtc tcggatcccg gatcaatacg gtcatgcagg ccgcgttctt
    27661 caagatcagc gaagtccttc ccgaggagga ggcgatcgcc ctgatcaagg agtacgtgaa
    27721 gaagacgttc ctccgcaagg gtgaagatat cgtcaagatg aactgggcgg cgatcgatgg
    27781 cgcctccgat gcgctccatc gggtcgagat ccccaagacg atcaccaaga gctatgagcc
    27841 ggttcccctg atcccggagg attccgaccg gttctcgaag aagattatcg agccgatcat
    27901 gcacctcaag ggcaatgaca ttccggtttc cgagatgtcc ttcgacggaa cgcttcccac
    27961 cggcacggcc aagcttgaga agcgcggcgt cgcgccgttc gttcccaagt ggatcgccga
    28021 gcactgcatc cagtgcaacc agtgcgtgca aagctgtccg cacgccgcga tccgggcgaa
    28081 gcagatcgag cctgagcgcc tgaaggatgc tcccgagacg ttcaaggttc tgaagtcgat
    28141 ctccaagaac gaccgcgatc tccagttcaa gattcaggtg tacaccgaag actgtcaggg
    28201 gtgtggcgtt tgtatcgaaa cctgtccttc gaaggtcaag agccttgagt tcagtccgat
    28261 ccaggtcgag cgcgatgccg gcgagatcgc caatgcggag ttctttgagc aactcccgta
    28321 cgacgtgctt gacggaagcc ccatcaactc gatcaagggt cttcagttca agcagccgct
    28381 cttcgagttc agcggagcgt gcgccggctg cggcgagacc ccgtatgtga agatggtctc
    28441 ccagatctgt ggtgatcgga tgatcgtcgc caacgcgacc gggtgttcct caatctatag
    28501 cggaacgttc ccgaccaccc cgtacaccgt tcgccaaagc gacggccagg gtccctcgtg
    28561 gggcaacagt ctctttgagg acaacgccga gtacggtctt ggaatgcgcc ttgccgtcga
    28621 tgccaaccgc gagcagctga agatctacat cgacaagctt tttgcacttg gaacgtccgc
    28681 cgagctgaag agcgcgatcg agaaatccct tgagctgtgg aaggaatcca gcgaggaagc
    28741 gaacgatgct caaaaagcgg tcaaggccgc ccttcccggc gcgatcgccg cggcgaagag
    28801 cgatgaagag cgcaagctcc ttggcaagat cgatgagctg aaggactact tcgccgacaa
    28861 ggtgatcttc atcatcggtg gcgacggatg ggcgtatgat atcggcttcg gcggtgtcga
    28921 ccacgtgctg gccagcggcc gcaacgtgaa catcctcgtc cttgatacgg aagtctattc
    28981 caacaccgga ggccaggctt ccaagtccac cccgatcgcc gccgtcgcga agttcgcgaa
    29041 cgccggaaag gagatcggta agaagaacat gggcttcatg gcgatgacct acggtcatgc
    29101 gtacgtcgct tccatctccc tcggcgccaa ccgcctgcac gcccagcgga cgctacagga
    29161 ggcggtggcg ttcgacggcc cttcaatcgt cttcgcatac gccacctgca tcaaccacgg
    29221 ggtgaatatg cgctacagcc aggagatcat gaaggacgcc gtcaactccg gctactggcc
    29281 gctgtaccgc tacaatcctt ccctcgagga aggcaagcgc ttcagctggg atgtccgctc
    29341 cacgccgggc tccttccagg acttcatccg agcggaggtt cgctacagct ccctcttcaa
    29401 gaccaacccg aagaacgccg aggcgctctt cgcccgggcg gaagaggatg ccaagcgccg
    29461 catggagttc taccagaagc ttggaccgct gatgtaagcg gcaatcagac tctcctcagg
    29521 ggctgctccg gcagcccctg tttcgtcaca tatcgctcct acttaacgaa tatcatgaaa
    29581 ttcgttacgt actattagcg tttatcatta tatgtgttaa ttaaagcggg taatttattt
    29641 aaaatagggt attctaaggg tgagaaatga ttgggaggaa acggttacat ggatgctgca
    29701 gagcgttacg ttaaaagaat attggatatc tcggctcctc ttcgaaggaa atcacaattt
    29761 ctctttggtc ctcgaatgac cggtaaaagc acacttattg aaaaagaact tgtggagaaa
    29821 cctgctttgt attggaatct tttgaggaca gggttgcaga tgcgggtaaa acagaatccc
    29881 gagctgctta cccaagaagt tgaagctcga caattgtcac attgtctgat cgtcatcgat
    29941 gagattcaat tggtacccga gctcttggac gagattcatt tcctcattga agagcgctca
    30001 attcgttttc ttttgtccgg ttccagttcc ggaaaactgc tcagggaaaa agggcctaat
    30061 ctgcttgggg gacgggcagg caacctgtca ctgcatccct tcgtgtatcc ggagatcaag
    30121 gagatgggcg tgactcttga gcatatcttt acgacaggtt tgatgcctca tgttcttttg
    30181 tcggatgatc ccgatcaaga ccttgatgat tatatcgccg tctatctgag agatgagatc
    30241 ggtcgcgggg gcaacgtgcg taaattggaa acatttgccc gttttttgga tgtcgccgct
    30301 cttacaaatg ccgagcaggt caactacatg aacagagccg gtgatgtcgg tataagcagg
    30361 cagtccatag ccaattggta tcaaatcctc atagacaccc ttatcggaca ttacctcccg
    30421 gcatatacca agggcgtcaa acggaaaacg ttcagcttgc cgaagttcta tctcttcgat
    30481 ctcggaataa cgcgggcatt actccagatt ccccctccgg tggagggaca aagcgaatat
    30541 ggacgttttc ttgagcatta tatcttcatg gaactgaagg cgtatcttga ttatggccgg
    30601 cggaaagaga tcctttccta ctggcggaca accggcggca ttgaggtcga cttcattctt
    30661 ggcgatcgga tagcgatcaa agtgaaagcg accaaacagt cgaatgcccg ggattataaa
    30721 ggcttgcttg cattcatgga agaaggaatc gccgaacggc atattctcgt gtgtcgagaa
    30781 gagcacccga gaaacctgaa gaacggtgtt gaaatccatc cctggacata tttcctcgaa
    30841 cagctttggg gcggaaagat cctttaggga gaactcacga tcaatcgagg aatgatttca
    30901 tttttcctca acccaccgtc tgaacatcac ctcggtcgag cggttcatct cccgcatgaa
    30961 cgcgctggtg tgcttgcgcc ggtaatagaa gtcggcgagg gttccggtgt tttcataggg
    31021 gaggctttgg atccttcgct tctgctcttc atgctcaaag tacgcatact ccatctcctt
    31081 gaggtggggc tcccggtagg aatccttcat gaagatcgaa tcgactgggc agcggggggt
    31141 cggttttatt gcgttgtctg ttttggggta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31261 nnnnnnnnnn gcgatcatcg gctggttgtc gcagaggagg gcaagcttct ccttcttctc
    31321 ccggtcacag acctcgacga tcgaatagta cgccatgttc cccgcggtag gagccctgag
    31381 ggtcgcctct ttgagggcaa gcaggagttc aggcgggatc ggtttgtcgg aaaacgcccg
    31441 gagcgaacga cgattgtgta tcaggcggag tgtttcattc atggtgacaa gtataggaaa
    31501 agagagtcgc ttgtaccacc acccggatat acagatatgc ccctggtatt gtatgatgaa
    31561 gaccatgaga agcgtcaggg tgtaccgcta ccggtgggtc gtgcttatca gcctcatcaa
    31621 cctcgtcctc gccggaagcc tgcagtggct caatttcgct cccgtagggc ggatcgtgaa
    31681 cgcttattac cgggatcaga tccttttgcg gttcttcaac ccgatcgatc tcctctccct
    31741 tacctatttg atcgtctacg tcctcttctc agtcccctcc ggctggatca tccgcaagct
    31801 cggcatgcgg gtcacctatt ggatcgcatc cggcctgatc atcttcggag cgatgaccaa
    31861 gactctgtac ctctcctcat tcaagaccgt tctgttcggc cagatcctcc tcggtctcgc
    31921 ccagacgttc acccttacca gcattaccga gacgacgagc cgctggttcc cgatccgtga
    31981 gcggggaatg gcggtgggaa tcattcaggc gagccagtat ctttctctgg ctctcgtcat
    32041 gatcctttcc cccctcttcg tcgttcgctc ccaaaagggg atcgagcagc tgatgaggct
    32101 gtgggcgatc atctcgatcg tccttgccgc cgtcagcgcg gctctcgtcc gggagcggcc
    32161 cccatccccg tcatcaacat tcgagcgaga agaagagatg aagttcagcc aagctctgag
    32221 caccgcgctc agccgccgat cgctcagaat cctcatcatg gagttcgcca catcgtgggc
    32281 ggttctgatg acggtcttca tcaagatcga tgaaatcagc gaatttctgg ggttcgttaa
    32341 ttccaatgga attctggcgg tgacaatgct catcttcgga atggtttcgg cggtcgtcat
    32401 tcccgccttg agcgaccact ttcgaaaacg gaaaatcttt tatacgttgt gcgcccttac
    32461 cgcggttccc ggcagcttat tgctcctcag cggcaatatc acggtcgccc tgatcggggc
    32521 ggcgatctta ggtagcgggc tcttggcctc aattccgatc ggtagccaat acgcaaccga
    32581 actcggacac ggaatcaacg aaaaagtcat ccaatcgatt cttcagtttt tcagccagct
    32641 ggccaccgcc gtcatccttt tcgtcacgac gatcatcaag gagcggtatc agctggttct
    32701 tcctgcgatt ctcgcgacga tcctcctggg ctccctgatt gcaacgttct tcatcaagga
    32761 gagcgaggtg atcatcaccg aagatgagcg cctcaaggcg ctcatcgatc tggaaaacgt
    32821 tcaccattaa gctatggctt ctgcggcagg agtcggtcgt tccaccgatc cttcgcatac
    32881 acatgatcca aaggcaggcg cacgcgcggg ctgcgttcgc ttttgaggtg atgctccttc
    32941 agggtggaat catcgccggg gtatccgatg atcaggaggg tcagaagcat ctcggaagcg
    33001 gggatcttca acgcttcctt caccgcatcc gcgttgaagc cggcgatcgg gtggatatgc
    33061 aacccttcgg agatcgcttg ggcttggtaa gccatcgtgg ccatccccgc ttcaaagggg
    33121 gcgaagaccc gatccccgat gatgatcgac cagtcgttgt tggttacgac ggcggtgatc
    33181 gccggagcgt tttgagccca gtagttgccc ggggtgagag tgttcttcac ttccgcaaga
    33241 acggcagggt cggagacggt gatgaagcgc catggctggc tgttattcgc cgacggggcg
    33301 gtaaccgccg cttcgacgag gcgggtaaga acttcttcat caataggttt tgaatcgatc
    33361 gcgcgatagg cgcgccgggt ttgtaatatt gacagtacag gcataattcc tccgtaatgg
    33421 gatcaatata ctcaagaaat aatgaatcgt cttcatacaa agacagggtc tgagattcca
    33481 gaccctgcca agtgcacatt gaattgctta atcgagtggc gagaagtcgg acttcggtgc
    33541 gccacagagg gggcataccc aatcctccgg aatatcagca aaagcagttc ctgccgctac
    33601 accattatca ggatcgccaa cggccgggtc ataaatatac ccgcaaagat tacattcgta
    33661 ttgcatctca tttcctcctg caccctagca taccgataaa gtatggttcc cgcaagaaaa
    33721 ttcgtctatc ggtagatcgc cagcacatcc tcaaggcgtt tcatgaagcg gcgccgatag
    33781 tgctcgggcc ccagaatctc caccgcatcg ccgcattgga tgatccgcaa ggtcagctca
    33841 atcgagttct cgacatccat gttgcagatc cacgaaccgt ccttcagttt ttccaattga
    33901 ggctttccgt gaaccagctg cctgaatgcg ttcatcgccg atcgctcctt cagcttgagg
    33961 ctgagaggaa tcatgtcgat cgcatcaaat gtctcgggag catcggggaa tttgaagggt
    34021 ttgaggttgt cggggatcgt gtaggtctca tccaggatcg tggcgctggt gatcgtggag
    34081 atatcgacgg tcacgatctc ggtggtgtgt ctcagtcgtc cggtgacggt aatcggatgg
    34141 cgggttccat cggtctccct gaagccgatg aaataggggg caagctcggt ttcgatcgaa
    34201 tcgaagcctt tgagcgattg aacgatccgg acgatccgcc cgctgaccca ggaatcgatc
    34261 agaacctcca gaatcgcatt gaacgcgcta ctctccttct gggcgatcaa ttgcttgtcg
    34321 atctgctcgg cgagcgaaac gatgttttcg ctgatcttcg gggcatagga gcgcatgttc
    34381 atcgcgatct ttctgagtcc tgaagcaaga tgagggtttt ggaactcgct ggtgttcagg
    34441 agcatctccg ccgagaggtt gatcgccaaa ctctcataga cggagagggc atggacctct
    34501 tcaccatcct ttgcccgaat cttcaacagg ttgttttcct catagaggtc gatatattcg
    34561 gcaagcttgt cgatgtagcg gctgatcgtc gagcggtgga cgccgagtct gcgggcgatc
    34621 tctgcccggc ggagtccctc gggatgagaa tggagaagca gttctatttg tgcaacacgt
    34681 tcgcccttca caacaccccc tttgcaccag aatgcaacaa aacatgggtt agtgtaccat
    34741 aagattgaaa gacggtaaac agaatgcaac atcctgttga ttcgaacccc gaagatgatt
    34801 gtggtatgat gggagtgcgg catatcaatg atgataagga gcgatcgagt gaagatttca
    34861 gcaatcgggt gttgtctgat cgactacctc tttcatgaag gaacctactc gaaatcggtg
    34921 ttcaacgagt atcggagcgt tgtttccggt gacggggggt tgctcgtcgg cggtctggtg
    34981 ttcggcgagc agcttgccga gttcacgggc ttgccgttcg atacgctact ccggcgcctg
    35041 attcctcatc cggaagaggg agttgaaaac atcggcggcc cggcgatcat cgccctgatc
    35101 cacgccgccc agctgctgta tgactcgccg cactccttcc acttccatgg tgtcgccggt
    35161 gatgatgtcg aataccgact gatccgggag catatcgcaa agacggtgat cgatccccgc
    35221 ttcctcatcc ttgaagggga gcgcaccccg acgacctatg tgctgaatga tcggcatgcc
    35281 cacgggggaa aaggggaacg caccttcgtg aatcttctcg gttcggcagc ccatgtcatt
    35341 gaagcggatg tgggggatga tgcgtaccga agcgacatcg tcctctttgg cggaaccgcc
    35401 ctgtggcctg cgttccacac ccggatcgat caggccctcc gccgagccaa agcggagggg
    35461 gcattgacga tcgtcggcac ggtcttcgat ttcatgaacg agtcccgctc atcaagcgaa
    35521 gcatggccgt tcggggagga gagcgggtat ccgttcgtcg acctgttggt gtgcgacgcc
    35581 ttggaagcgg agcggatgag cggccaaagg gacttggaaa aagccgccgc cttttttgac
    35641 gaggcggggg ttggtgccgg gatcatcacc cacggatcga aagagatcat cgcttggtcc
    35701 aaaaaggaga gcgtcttcgg acatacccct ttgacgcact tcccggtgtg tgcccatatc
    35761 gacgagctgc tggaaagaga tccatcgctt cgcaaggaca ccaccggatg tggagataat
    35821 ttcctcgggg gagtgctcgc ctcgatcatc gctcaagggg cgaaaaaatg cagcatggtc
    35881 gatgcgatca ccctcggtgg cgcttcgggg ggattcaccc tgacccatca cggggggatg
    35941 ttccatgaaa cctacccggg tgaaaaacgg agcttgcttg aaccggtgct tgccgcctac
    36001 agcgggcagc tcggtgtgct ataatcggat catggatgat cgcttaatca atgtggagat
    36061 gcaactcacc ttcgcccaag agacgattga gaccctcaat ggggtcgtca cggaacaggg
    36121 gatggaattg cagcggctga aaacccttgt cgagaagttg gaacgccgga tcgtggatct
    36181 cgtggaagga agtgaaggcg gcccgcaaaa gccgcctcac tattaattca atcgagcgcg
    36241 ttcactgatc gccgttgtgt aggagcgaag ccctccggtg aggttgtaca cctccgtaaa
    36301 gccgttcccc cgcagaatcc gttcggcgag gtatccccgc gttcccatca cgcatacggt
    36361 gatgatcggc ttgtccttcg gaacctcggc gatccgattt ctcaacgcgg tgtgtggaat
    36421 gttgattgag ccgggcagca ttccccccgt catcgtttcc atttcacttc gagtgtcgag
    36481 gatcaccgcc ccctgctcga tgagattgtc gacttctctc cagctgatcg ggttggagat
    36541 cccttcaagg gcgttcatcc cgatgaagcc gaccatgttg atcgggtcct tggcgctgga
    36601 gaagggagga gcgtaggcgt gttcgaactc ggcgagatca tacaccgttc catccattcc
    36661 gatgaacgag ctgatcacgt cgatccgttt gtcgaccccc tcgtagccga tcgcctgagc
    36721 cccccagatc ttcccggtgg tcggatggaa caggatcttc aagctcaggg agaggctgtc
    36781 tgggtagtaa tcggcgtggc tggatgtatg ggtgactgcg ctcttgtacg gaagctccaa
    36841 agcggcaagg gtcttttcgg caagtcccgt cgaagcgacg gtgagatcga agaccttggc
    36901 gatcgatgtg ccgatcgttc ccttgtaggg cttcctgttc ccatcgatga gggcatcggc
    36961 aagcagacgg gcctgcttgt tcgcagggcc ggccaagggc accgtgatcg aatgggaggt
    37021 caagggacaa gcgaagcaga tcgcatctcc cagcgcccga atggaggggt cattggtagt
    37081 gaagtattca tccaccacga tcgctcccgt tgaagaaagg acgatcccgc tttctttcag
    37141 gaaggtcgta tccggccgga ctccgatgga gaggatcacc agatcggttt ccagcacctt
    37201 tccactttcc aattcgacaa gcaccgcctc gccgttcttg ctgaagcctt tgatcggatc
    37261 cttgagatgg aggacgactc ccttttttct catgtgttgc tgaacctgag cggcaaaatc
    37321 atagtcgatg acgcccatca cctgttccaa tgcttcgaca agggagactt caagcccccg
    37381 gtgtttgagg ttctccgcca tctcgaggcc gatgaacccg ccgccgatca ccaccgcccg
    37441 ctttgtttcg ctgcggtcga tcgacgcctt gatccgatcg atatccttga ccgagcgtag
    37501 cgtcatgatt gagggatgct caatgccggg aatccgcggg cggatcgggg atgctccggg
    37561 ggagaggatc agatggtcat atgcttgtgt ataggtggtg tcatctttga cattgcggac
    37621 ggtgatcagc ttttgggctc gatcgacgct catgacttca ctgttgaccc gggcttcgac
    37681 gttgagcacc cgcttgaaga gttcgggggt catcacgaag agggatgacc ggttttcgat
    37741 gacgccaccg gcatagtagg gcaggccgca gttcgcaaag ctgatgtgtt cgccccgttc
    37801 aaagataatg atccgtgcct tctcgtcccg tcgtctgaga cgagcagcca ctccggctcc
    37861 gccggcgact ccgcctacaa tcagatatgt tgccatgatt cctcctgagt aaaggccaat
    37921 tcttcgggga tctgaagttc acagcagtta taggcaagaa caagctcttc tcccgcttcc
    37981 gtcaaactga tcaccaaact ccttcgatcc gtgattgaaa gagttcgctg aaccagcgat
    38041 cgcccttcca ggctgtcgat cacgcgtgtg aggcgtgaag gtgagagatc caattctttc
    38101 gccaatgccg aaggctcgac gattcccttc tccaccgcac aaaggaggag agcgtcattg
    38161 aaggaaaggc ccgtttcatc tttcaaccgc tcttcaaagg agcgcagagc gctttgaagg
    38221 cggcgtatgg cgcacagatt gagcatctga acctccggat aatttcgttt gtcaataatt
    38281 gctttagaca actataatga tgccgtatcc ctccgtcaac agatctgata ataaccttat
    38341 aggtaaagaa tatctgcata tagatactca ttctcattaa tgtacccgat aatcttatat
    38401 ccactataag gataagcgtt tgtctcttga ctattgtttc agaatccttc atgctgtttt
    38461 caggttgttc tagatgttgt ggcaacgcag tgtcgggaag aagccacttt gttgcttcct
    38521 tgttctactt ctagatctac accggatgga acacaatgga taggacgcca aggcgtgttg
    38581 ctttttgcgt ttgcaatcac accaaatatg gtcgatagtc attcccaact atcggccatt
    38641 tctttatgta agcggttaca ttctcagagt ggatgtattt gttgatcaat tcccagtata
    38701 gtacaagtat gaaaacaatc ctgtgctacg gtgattccaa cacctatgga tcgaaccccg
    38761 gcgggggacg atgggaatat gacaagcgat ggccgggaat cctctcttcc ctattagggg
    38821 atgggtacta tgttattgaa gagggacttg gcggacggac aaccgtcttc gacgatccgt
    38881 tcgagaaagg acgaaacggc aaaacctttc tacccgtcgc tctggcaagc catcagccga
    38941 ttgatctttt gatcatctcc ctcggcacga acgatacgaa gcgatttttt ggcagcaatg
    39001 aacggatcat cacccgtgga atcgaagcat tggcggatgt tgccaaaacc tttgatgtca
    39061 aacagatcat ggtgatctcc ccgatccatg tcggagagag gattgaagag actgtcttcg
    39121 ccggcttctc cagggaatcg gtggaaataa gctgtaagtt gagtgaacgg gtgaaagcaa
    39181 tggccgaacg aaaaggcctc atcttcttcg atgc
//
LOCUS       DAOSLI010000013        36981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1287_length_36981_cov_26.982153, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000013 DAOSLI010000000
VERSION     DAOSLI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 36981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36981
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_36981_cov_26.982153"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..202
                     /locus_tag="PLF67_03300"
     tRNA            129..202
                     /locus_tag="PLF67_03300"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:163..165,aa:Asp,seq:gtc)
     gene            258..1328
                     /gene="mnmA"
                     /locus_tag="PLF67_03305"
     CDS             258..1328
                     /gene="mnmA"
                     /locus_tag="PLF67_03305"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOE89076.1"
                     /translation="MRVLLGMSGGIDSSAAAYILKNQGHEVIGVTMTIWSNRAHLQRP
                     VDSVSCFAPDKSEDIERIKELCARLGIEHHTLELSDRFEEEVLSNFKDEYLDGKTPNP
                     CVWCNQRIKFGAMVEYAKKSGLVFDKFATGHYARIVHTGKRWAVGKAKDEKKDQSYFL
                     YRLSQEQLKTILFPLGELAKDEVRVILKEHGHVSDDQEESQDFYDGPYTDLLEVEDKE
                     GAIITRDGRILGTHKGIWHYTIGQRRGLGIAAERPLYVLELRASTNEVVVGFVDETVQ
                     HQVEATDVVFGSVAMIEGEVEVEAKIRSVGRPKKAIARRKEDGSIVAFFPEGVNAATP
                     GQSLVLYKDDLVLAGGIITHAR"
     gene            1378..2169
                     /gene="nagB"
                     /locus_tag="PLF67_03310"
     CDS             1378..2169
                     /gene="nagB"
                     /locus_tag="PLF67_03310"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOE89077.1"
                     /translation="MRVVIQKDFLAMSKWAAKLIADRINDFHPTEDRPFVLGLPTGST
                     PVGTYKELIALYKEGYVSFKHVITFNMDEYVGLGSDHPQSYHTFMWENFFNHIDIKKE
                     HVHIPNGMAKDLEEESLRYEEAIEAVGGIHLFLGGVGGDGHIAFNEPYSSFDSRTRVM
                     TLTEHTRQANSRFFENKIDLVPRYALTVGVKTVMSAKEVMILISGANKAQALHAAVEG
                     PITQAWPISTLQLHKEAIIVCDEDACGELKVSTYNYFKALEKAMV"
     gene            complement(2166..2975)
                     /locus_tag="PLF67_03315"
     CDS             complement(2166..2975)
                     /locus_tag="PLF67_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOE89078.1"
                     /translation="MSKTHPFFDPMPRIVAHRGDSGSFPENTLPAFESAVAMQVDVIE
                     TDVHISKDGHIVIWHDPTLDRNTDASGRIEDHTLDELKTFDAGYTFTVDGGKTFPFRN
                     KGVRLATLKEALETFPATRFNIDLKSKEPEIVDAFIDLIRKKDAFDRVIGASFHLKNL
                     QALRSKEKRIQTSLTTLEVIPLLAKQKLGLLSSKKQKGLTIFQVPPRQWGIEVVTPRF
                     VEIMHKRGAIIQVWTINEKEEMKRLLVMGVDSIMTDHPQLAIEVAKELGLR"
     gene            complement(2950..3708)
                     /locus_tag="PLF67_03320"
     CDS             complement(2950..3708)
                     /locus_tag="PLF67_03320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HOE89079.1"
                     /translation="MTHPSRLDHIQIVLVDTQDGANIGSVCRAMKTMGITRLVIVGER
                     EYEENRIRTLALHAGDVWENRVQHPTLQEALKESSYTVAATRRRGKFRKHTFLSPLQL
                     AEKIAHTEGTVSIVFGRESDGLTDEEVAACAQVVSIPTSDAFPSLNLAQAVQIITYTL
                     FEAHLPHPTGLIPITQERAQRASDAAVDALSEIGYFKLDQEELWTRRFLKDVFTRASL
                     SESEVQRMEKLFTKMVRIKIHKKEKPDEQDPPVL"
     gene            3763..4995
                     /locus_tag="PLF67_03325"
     CDS             3763..4995
                     /locus_tag="PLF67_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOE89080.1"
                     /translation="MNESTSVGNRYIHIMLGIIVTLAVLAALKISKDVTIPLVLAFFF
                     FLMFSPLLRRLDKLHVPKLISTIFVMALMLLLLLAAGWFIVKTVDTLVRLIPFYTDKI
                     ISLDRLLTDMVNNFVEVPPSTSFLTLLPVNWSSLAISSLTQISNKFFSITKLALLVYI
                     FFLFLLLERQSVVPKLLAAVPQGKGMKVAVMFERITRQISKYLVIKIVISFFTGLFFY
                     LSALLTGLDLASLWGVLAFIFNFIPSIGSIVITSVVIFMALVQFAPDWTNIILVAVLT
                     ISTQMILGNIIDPRLQGGQLNLSPFMILVALSLWGYIWGIVGMFIAVPLTSVMQILFA
                     NIKSLRPFAIMISSGKSYQRERARQKALKSYQRRTQHRKSEQPAGEQGNESSEDSMAK
                     ESVNMGDFVLPEQFGEKK"
     gene            4992..5858
                     /locus_tag="PLF67_03330"
     CDS             4992..5858
                     /locus_tag="PLF67_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HOE89081.1"
                     /translation="MIEFPSLMEEDARRYLLPSNPSYDALIETVLTFYRDWGRRFVFR
                     DRHDPYSVLLGEVMLQQTQTSRVVDKYRYFLAEWPSFKALARAPLEEILIAWKGLGYN
                     RRALNLKKAAEKTEAWNWTIPNDSKAMLTLPGVGKATAAALLAFCYQEKSIYLETNIR
                     RVLLHYYFPDQTDVHDRALEAILSELIVLIDDPRSWYYALMDYGVLLKALVPNPNTKS
                     AHYAKQSRFEGSKRQVRAALLHCVAEQGPIALSSVTEILKEYDSTYLAQSVEELIKEG
                     FLLLEKDSLSIR"
     gene            complement(5809..6750)
                     /locus_tag="PLF67_03335"
     CDS             complement(5809..6750)
                     /locus_tag="PLF67_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE89082.1"
                     /translation="MNQGIYLALDGLTGAIISVMVVANTNLGHATTMGVSLLVNHLIG
                     FTLVSTILFFGRNNPAIAGPKRRAPWPLYLNGMLGVLILNFNFHSFTALGSSLAMALT
                     VFGQSVLSLVFDLNGWLGMKKRSFSPKKVLSLAVSGLGIVVMASKGVGSFSWIYILIA
                     FAAGAITITQMILNSTFAIYKGPMKASQWTYIAGMSAAVPFYFLTRPSATIEGFARLP
                     SVPFIQIIAGGVLSVTVLISTAFVIVKLPAIYSALLLSASQILASVAIDAFFLKIFSP
                     NLLFGALLMLLGMAGNLLADRSTGSKENPSQEGETLP"
     gene            6811..7815
                     /gene="cobT"
                     /locus_tag="PLF67_03340"
     CDS             6811..7815
                     /gene="cobT"
                     /locus_tag="PLF67_03340"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOE89083.1"
                     /translation="MDYRKLLLKKAMIPGSLGFLGDLALQLAKFYPRPLDTFSLLLFA
                     GDHGIHGEGVTYSPQEITWQQTRSFASGGGACTLFAKLNGIDLKIIDVGVAGSFDPST
                     SVIDRKVGFGTKNFLHEQAMTDKECASAIEVGRAMSRSSGADLIGFGEMGVANTTSSS
                     AIVAALLDRKPEEVTDGGSGLSEPDLRHKIEVIRKGLAFHPRRDPLDVLSAFGGFEHA
                     AIVGGILEAYTMKKPILLDGFVVGAAALVAAMIDPNVKQNFIFAHRSGMKGSGILLEA
                     LGCTKPILDFGMYLGEGTGALTAWPIVRQASHILWEMSEFEQAEVTDSTAILRSKGLL
                     "
     gene            7812..8705
                     /gene="cbiR"
                     /locus_tag="PLF67_03345"
     CDS             7812..8705
                     /gene="cbiR"
                     /locus_tag="PLF67_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HOE89084.1"
                     /translation="MIRLGTTSYIIEDTLGGNVRYLAPMVDDVELVLFETPEASNIPN
                     TAEIDRLNGLASEYDLTYTIHLPVDARPGSRDEKVRASSVDLIGRVIEQTRVLKPFGY
                     ILHLAPERYGPIPAEDVRTWLIQSEKSVIDLLQRTGVNPLLLGVETLGYPLDLVDSII
                     SRHGLGVTLDIGHLHLMGYDVEDHVKRYASRCRVFHLHGVNEGTDHLGLHRGDREKID
                     RFLDLLASLEDGIERVVTLEVFDRESFEGSMEVLHGRNGGEKDGYLGDRGRTKREKHV
                     GGSIRSIPWREAGLSGDRRSV"
     gene            8593..9108
                     /gene="cobU"
                     /locus_tag="PLF67_03350"
     CDS             8593..9108
                     /gene="cobU"
                     /locus_tag="PLF67_03350"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOE89085.1"
                     /translation="MVTLVIGGGRSGKSTWAEAYARSLGGKRVYLATAEAFDEEMKER
                     IRAHQARRSGQFITIEEPLQLSGRIMNLPSDVRVCLVDCLTVWLGNLMHYEKDLEAYL
                     EELYRVVDDPPCELILVTNETGLGIVPADPMSRIFRDRAGWMNQRLALLADRVVLLVA
                     GIPLAIKGTLP"
     gene            9105..9836
                     /locus_tag="PLF67_03355"
     CDS             9105..9836
                     /locus_tag="PLF67_03355"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOE89086.1"
                     /translation="MSLASALRLLTRFRIPGKEIEQSGRTLFWFPIVGALLGCISCLF
                     ALLPLSAPIRSVLVLIGGVYFTRALHYDGVADLADGLGGGFTKERSLEIMRDSHIGAF
                     GTIALMLVVLLQYSLLLDVVEHPPMLILVSTLGRLAQVWAASLMNYARKGEGMASSLV
                     RRAKWHHALLPTLQVGLLILLLFVLHHPYAIPVLAAFGGIVLSTTILCLISRKRLGGI
                     TGDVLGSVEVFGETAALLGIVFALA"
     gene            9880..10482
                     /gene="thrH"
                     /locus_tag="PLF67_03360"
     CDS             9880..10482
                     /gene="thrH"
                     /locus_tag="PLF67_03360"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HOE89087.1"
                     /translation="MKIVCLDLEGVLVPEIWINVAKRTGIEELRRTTRDEPDYNKLMR
                     RRIEILKEHSLTLSDIQAVIASMGPMEGAKAFLDELRSLTQVIILSDTFVEFAKPLMA
                     RLDWPTIWCNSLEVKDGMITRHKLRQEEGKFHAVQALKALNFSTFAAGDSYNDLKMIL
                     EADEGCLFRAPKTIIESYPHLVLTTTYGELMDRIKEFLTS"
     gene            10479..10700
                     /locus_tag="PLF67_03365"
     CDS             10479..10700
                     /locus_tag="PLF67_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89088.1"
                     /translation="MILRPVIFLVLAILAGFSLLGVLWEGSVFIKVVVGLTVAAFSVG
                     LLIVARRFDRALKEKRGDADDAENGGISQ"
     gene            10672..11514
                     /locus_tag="PLF67_03370"
     CDS             10672..11514
                     /locus_tag="PLF67_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HOE89089.1"
                     /translation="MRKTVVFLSDFGSSDGAVSAMHGVADQVSADLKLEDLTHDIPQF
                     NIWEASYRLAQTLPYWAPGTVFVCVVDPGVGSDRKSLVALTNDGHIVVTPDNGTLTHI
                     ERRIGLKEVRELHVETNRLAGSQKSHTFFGRDVYAYNGARLASGELAFRDCGPVLEEF
                     IRLEVEEQRREGETIVGAIDILDTRYGSLWTNINGDLLDEIGVTWGDYLHVRITKDNR
                     IVYGYPLRLCKAFTDVPKGEALMYINSLLNIAVALNQGNFASTYSIGTGEGWKITFSK
                     YDTP"
     gene            11527..12093
                     /locus_tag="PLF67_03375"
     CDS             11527..12093
                     /locus_tag="PLF67_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HOE89090.1"
                     /translation="MAKAKGLDPVRFVVIVAIGAALYGLGGMIGVPIFANTTLKPAMA
                     ILGLFAALYGPIVGFLVGFLGHWITDLFMGWGVWFTWVLGSGLVGVMIGLFKYWSKDC
                     VHEGNLPGMQILLFIVISFVANFLGYLVSALLDALLYTEPLDKVFTQQILAATTNTLM
                     IATLGTLLLYLVARRNASSRDLKKDEKA"
     gene            12136..14769
                     /locus_tag="PLF67_03380"
     CDS             12136..14769
                     /locus_tag="PLF67_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3744 domain-containing protein"
                     /protein_id="HOE89091.1"
                     /translation="MAEVLISFKEFSFTYKAQSEPTLKHINLSIHRGEKILIAGPSGS
                     GKSTLGYCINALIPHAFKGKIEGNATICGLNLADGDMYAVNKRVGTVMQDTDAQFVGI
                     TVAEDIAFSLENQCVEQREMHSTVAEIARVVKMGSFLSHSPQELSGGQKQRVSLAGVL
                     VDDVDILLFDEPLANLDPATGALAIEMIDELANLHNKTVIIIEHRLEEVLHRKVDRIL
                     VMQEGKLVSDTRPKELLASQTLRALGLREPLYITALRMAGCDLKRAGDLSSYEGVKLD
                     SVKEAIHDWYAVSQKDSAARERDVLLRLENLSYSYDGLVMAVEGISASIHSGEMISVL
                     GNNGAGKSTLAQLIMGVLRQDSGSISFEGIDLAGYSAGARSDLVGFVMQNPNHMISND
                     LVFDEVAFALRQRGYEETLIRERVLDVLGLCGLREYHHWPISALSYGQKKRVTIASIL
                     VTDPKILLLDEPTSAQDYLHYTALMQFLAELNEKTGLTILFITHDMHLALEYTTRSLV
                     LSGGKLLADLPTSEVFSNPDLLSRANLKETSLYYLAKDLGIADIPLFIEHFVQEERKE
                     RPPSVEVIRLPKRKLALTERKKEIKVGKKVVKEGRKFGFALSWEDTDSYLHSFNGVTK
                     IAFFGLWIGFCLLTFDLRFLLPALGFSFYLLWATKVPLRKFLPYIIGILGVIVLNAIF
                     IFLFSPKQGVVYLGSETLLLGNEMMRYALSRETLFYLFVVCVKYLTLFPVALVFVSIT
                     HPSEFASSLNRLGIGYTVSYAVSLALRYLPEVTKTYVNILRSQMARGVDISKNAPLRK
                     RLSSMARIIAPLVLFSLDRIEVITNAMVLRGFARLKKRTWYMQSSLRPRDWVALGLTL
                     LFVAASLYIRFARNVMFWYPF"
     gene            14887..15246
                     /locus_tag="PLF67_03385"
     CDS             14887..15246
                     /locus_tag="PLF67_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOE89092.1"
                     /translation="MADTFIDEMEELLTTMRKELLEKMSEDNSDFKSMVNAMGGKDSV
                     DIAADDIAFKKMEAINKHEANRLRSIDNALSRIHNGKYGLCLKCQKKIPEERLRAIPY
                     AVLCVDCKLAEEKPGRR"
     gene            complement(15302..15943)
                     /locus_tag="PLF67_03390"
     CDS             complement(15302..15943)
                     /locus_tag="PLF67_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE89093.1"
                     /translation="MILRRLVVGEYQTNCYILGSEETKSAWIIDPGTDGELIIDELTS
                     LGLTARSALLTHTHYDHIAALTRLKEHYPDMEVLLGKEDAKGLDYEVIKATILDRRFF
                     TIHEEALKHLPKPNDYLIDGQYLEDSRLSVIATPGHTPGGRSYYHREGRFIFTGDTLF
                     ARAIGRTDLPGGDYPTLIKSCYRILELEDDVRILPGHGPTTTVGDERRKNPYL"
     gene            complement(15946..16482)
                     /locus_tag="PLF67_03395"
     CDS             complement(15946..16482)
                     /locus_tag="PLF67_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOE89094.1"
                     /translation="MSNETNSTQEDKELIESTIQLLVTKVHADSEPEELEALAKTIKK
                     QVPLFTRRYFTAYLLREILKANTPKKAPAPKKAPAPQKSAPPQPRRGSQNQPLPEGAR
                     TLYLNIGKMKRLYAKELSELLQRELGIKREDIYSLRIHDKYSFISMSEQHCEEAISKL
                     NGMDIKGRTAAVSYSNRE"
     gene            16705..17679
                     /locus_tag="PLF67_03400"
     CDS             16705..17679
                     /locus_tag="PLF67_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89095.1"
                     /translation="MSTLQSQIQRLSRAIEEHRRDLRTLYGDLGKATATDDSPSAQEV
                     LQKKREYEIQEELYQQLTFSLKQFEDRSRKLKQVQKDLKVLSKEKKELCAQLGAIAYE
                     AYGNASYSDELSKTLAPFLAVRKRRNAGAPVFAHLHRRALGRMFLKLGEEILTRHLEE
                     ELRSEAREELFKQVREYRSKEHLLGEELALHQSALDQLKNSEVPTPWLRLETYNQSRM
                     KALKAYEEAAIAYGRQMYEHEGDQNPLATQITLHRTRIKQLGGEIKEKQNRIKIQELE
                     AQIEIEKQKIEHIADQIGALRAQIDQLNSAIAKRRTLIRMLEGTGNDG"
     gene            17672..18856
                     /gene="mltG"
                     /locus_tag="PLF67_03405"
     CDS             17672..18856
                     /gene="mltG"
                     /locus_tag="PLF67_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOE89096.1"
                     /translation="MADSKNSNVTTRSMKVTIGEKKSQVPSKKRNDEEKEPVKPHPPI
                     KNKSEGKVALRKPIVPPPIGKRKGYRRPMKELLLISLLAIVILAGMGAVGWNISRKAP
                     FDDEVVAVTIEAGMSATEVSFILEEAGIIDDRKRFLDYVVSRQAASLLKSGTYILGKN
                     STHEEILKALSSTGRVLELLVYPGYTLKQIDEMLTVRTGSKPGAFVKAAWEVGSAYGL
                     RFVEGWFLGGTYPVEIGERSARDLALSMFFATEEVVRTLSDSPLLDRYSIEELIIIAS
                     MIQAETQDQSQMGGIASVIHNRLEKNEPLGIDATTRYELDDWENPIPVEALEQKTPYN
                     TRRKTGLPPTGIAAPSKAALHAAFYPETTPYFYYLHGFDKRIHYAVDYEEHKQNIEKY
                     RK"
     gene            complement(18886..18970)
                     /locus_tag="PLF67_03410"
     tRNA            complement(18886..18970)
                     /locus_tag="PLF67_03410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18934..18936),aa:Leu,seq:caa)
     gene            19089..>19270
                     /locus_tag="PLF67_03415"
     CDS             19089..>19270
                     /locus_tag="PLF67_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89097.1"
                     /translation="MLKKILAIIFVLLFTIGISAKDLMLADLPIWYGKERFVERIEER
                     TGGIREPIGLVLSGGSA"
     assembly_gap    19271..19370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19371..20588
                     /locus_tag="PLF67_03420"
     CDS             <19371..20588
                     /locus_tag="PLF67_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89098.1"
                     /translation="DSFIWIRNDVESISFMEFAKVLYLQEKGYEAAQANGEVLGRLKR
                     GISVSSFAEKRERLDEAIRAADRQYAAFEHLRRFDDPQLLGIGLWSTITGGVRPYLLD
                     DFTMGISYTASRQALSFELNGGFAFNWARSGQMRTVPAIKTRLYYYFLDHWRVAFGGG
                     VFYDARKEGFLFSYDGLLEGRYSLGRIADLRISQTFEGLFNDRNSRYVEAFPGSTCLA
                     TSTVTLELPSLKWLEKGSVDLAYQAYGDYQRIRSFLNPYGSVKIRDPFIGIYLEGKTS
                     VRFALDGKGDVPLFISDRFRTNHDPLIIQGHDLTVSSNPSKYLAVVNLGFGYQPVSYR
                     PTFGEMLLFEDSRVGVFADLLFNGDFFFSVGLEAETTISLLGITELGIRFYGGWDQAS
                     RNPIWGVYFTVPH"
     gene            complement(20572..21618)
                     /locus_tag="PLF67_03425"
     CDS             complement(20572..21618)
                     /locus_tag="PLF67_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilF"
                     /protein_id="HOE89099.1"
                     /translation="MKKNLGLYISIAGTILIVALPISRTAKLIILFGAFAGLLFYRRG
                     VLYFGKANKKLQSGALDEVWVLYRKALKAGVAPNVRIAIASIFIQRGDVDEGKTILES
                     YKNLTKGQTKELDAIVGILDSMVHWIEGDTKAALKTVREVYEAGFRDSNLLVNYTTFA
                     LVERKWKEAENLLDEADEKNIVSAGLIDNRAWLAISKNEWVEAELILTDLISKGPRFA
                     EPYLHLAQVKIHWGLIGEAIDLLAEGLKKPFVNTSGIKASYIQDLHDRLSDPKTRRKT
                     AMEIDQDRLAVASGQEVKAIDRTFEPEEGLILSGFAKRPAGRKKQKVEPEEQGPNLEL
                     TAEDLAYIEANEEQ"
     gene            21716..22243
                     /locus_tag="PLF67_03430"
     CDS             21716..22243
                     /locus_tag="PLF67_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOE89100.1"
                     /translation="MKRRDILVHACCGPCSTSSIERMLGEGWNPVLYFSNSNIFPFEE
                     AQKRFDELMKVAKIYELEVIREIYDHESWLEAIRGFEKEQEGGERCRNCFAYNLAEAN
                     AKAIQLGFDHFTTTLTVSRFKHSPTIFAVGKQYERFEAIDFKKQGGFERSNILSREYG
                     LYRQQYCGCEFSIRP"
     gene            22273..23805
                     /gene="cls"
                     /locus_tag="PLF67_03435"
     CDS             22273..23805
                     /gene="cls"
                     /locus_tag="PLF67_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOE89101.1"
                     /translation="MRKILKFLTSRLFISFLLISIQIGFFVLIGIYAQSYGVWFQLLS
                     ALSVLMSLFVITRDLNPAYKIGWMFLFIVIPIYGGLFYLLFGTHKLNRRLRARVEELD
                     FSFRKGMKDALYSDLLPIRALESYSPSLARQAQFICSITGNQVHVNTQVDYFASGEAW
                     FDDVIIELQKAKSFIFLEFFILAEGEMWDLLLSILLERRKRGVRVCLMYDDVGSIFTT
                     PSHYDRTLRGLGLEVVAFNPLRTHLNSRLNSRDHRKVIVIDGNIGYTGGMNIADEYIN
                     RTIKYGHWKDTAVKITGDAVWSLTQMFLQLWSFSTGVTEQFALYQPTITAKTDGFVQP
                     FGDNPLDSHNVAENAYIQVINMAKRYVYITTPYLVLDNEMLTALKIAAQSGVDVRIVT
                     PYIPDKKGVFLVTRANYYKLLEAGVKIYEYTPGFIHAKMFVSDDHVAIIGTINMDYRS
                     FYLHFENAVVLYGSSAIKHVYEDIERTIMLGKPITTKLLKARPWHQRLGGMILKIAAP
                     ML"
     gene            complement(23864..26203)
                     /locus_tag="PLF67_03440"
     CDS             complement(23864..26203)
                     /locus_tag="PLF67_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOE89102.1"
                     /translation="MSIHREKAATLLKKMHLEEKVAQLVSVWFEIHDEQTITVREAIP
                     TYNHLPNPMEAALEFGVGQLTRPYGTIANDPQKQVKVINKIQRHLVENTRLGIPAMIH
                     EECLSGVMSVGATAFPCSLNYGSAWDTELMKKIGMAIGDELRSMGVHQGLSPVLDVSR
                     DARWGRLEETFGEDPYLIGEMAIAYINGLQGKNRQPLATLKHFIGHSGSEGGRNHAPL
                     HSGMNELLNTYGLPFEKVLKHTAVGGVMPAYHDIDGIPCTSNVELVVDLLKKEWGFDG
                     LIVADYEAIAQLEKDHHVAKDPAEAAALALKAGMDIELPGFTVFKKGLIAAFERKLIT
                     MDEIDRAVMTVLAEKYRQGIFDNPYIDEEGLELSSDEHHALAVEIAQKSLVLLKNEEL
                     LPLQRGKKIALIGPLGNHPYAMYGGYTAPVHLQGSAKWEETVPKRSKTIKEALEAYAE
                     VEFQPGCVIFESSIERSIFFPGEVEQQDGPLAKVPSTDTSRIIDAVKTAQNADVVVLV
                     VGDLVGLFQQGTTGEGSDANTLKLPGVQEELLEAILATGKPTVVLLVSGRPYTVNSAV
                     KRAGALVAAWLPGQGGGEAIAQTLFGEKNFSGKTTLSFPFSAGAMPYTYNHSKKSGGL
                     PRQSAFGARYPFGFGLSYTSFTYPAAKLKTDTVDLDGEVEVLVTVKNTGTIDGEEVVQ
                     LYIHDRHASLVRPVKELKGFARVPLKAGEEKTVTFTFKTDLLSFYKNGKRILEGGEFD
                     LFIGSSSRDIHHSFLLKVKDGVRILDFNSWNDRSHVMIT"
     gene            complement(26296..27150)
                     /locus_tag="PLF67_03445"
     CDS             complement(26296..27150)
                     /locus_tag="PLF67_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89103.1"
                     /translation="MSTHGSIRSKRMLYKTIVYLVCTFLAILSIFPFWIMFMNATRST
                     YEIQQSSIGLVPSKYFMSNWKILTGKAFSPMTGFINSMTVSVGVTVLAVYFSSLTAYA
                     LMAYSWKLRQAFFSFIMAIMMIPAQLSSIGFYQFIYRIGMTNKFTPLIVPAIASPAMV
                     FFMRQYLMAALPLEIVDSARIDGSREFATFNRIILPIMKPALATQAIFSFVGSWNNLF
                     IPLILLTNKKKYTMPIMVSLLRGDIYKTEYGSVYLGLSLTVLPLFVIYFMLAKYIIAG
                     VALGALKE"
     gene            complement(27147..28127)
                     /locus_tag="PLF67_03450"
     CDS             complement(27147..28127)
                     /locus_tag="PLF67_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE89104.1"
                     /translation="MRKKSVSYAKYGYIFSIPFVVTFLVFSLYPIIYTLVIGFTNLRG
                     LGVKEFHFLRTAEGNLDLFGNFRTVLNNKTFTTSLKNTIILWTANFIPQISMALLLTA
                     WFTNRNFKVKGQGAYKILIYMPNIITAATIAILFNSLFGYPKGPVNDLLMKLHIIDGP
                     RNFSLQRWTARGIVSFIQFWMWYGNTMIVLIAGVLGINPSLFEAAEIDGATPTQIFFR
                     VTLPLLRTILLYVLVTSMIGGLQMFDIPKLFLNGGPDQATVTSSMFIHDQAFSGSYLY
                     NRASAASIIVFGIIIVLSIILFQVLKDKDEAARRKYRRAQNRAARKGVNA"
     gene            complement(28216..29562)
                     /locus_tag="PLF67_03455"
     CDS             complement(28216..29562)
                     /locus_tag="PLF67_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE89105.1"
                     /translation="MAKRVRTITLVLLVAVLGVSLFAGGQTEATTAKKKVINVWSFTD
                     EVPKMIDRYKELHPEFDYEIKTTIIATTDGAYQPALDQALIGGGADAPDIYAAEAAFV
                     LKYTQGDAKQYAATYKELGIDVDRLVKQADIAQYTIDIGSNERGLVALGYQATGGAFI
                     YRRSIAKAVWGTDDPQVIKTKIGPGLDKFFEAAEALKKKGYAIVSGDGDLWHAVENSS
                     DKGWVVDGKLYLDPKREAFIDHSKRLMDNGYHNDTQDWTDAWFADMKGANPKQAFGFF
                     GPAWLINYVMAGNSGGTKIGEGTYGDWAVCEPPIGFFWGGTWVLANRDSKVKDAVAEL
                     IQWITLDSSETGLQYYWANGTLFGAGGTKDSVASGTVMSKSDGSLDFLGGQDMFEAFV
                     PAGAFATGKNLTQYDETINSTWRAQVREYTAGNKTRAQAIADFKRMVFETLDIPSN"
     gene            complement(29737..30393)
                     /locus_tag="PLF67_03460"
     CDS             complement(29737..30393)
                     /locus_tag="PLF67_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE89106.1"
                     /translation="MRKPTTFLIVDDHPLFRQGLVSVVEDNPHYMVIGQAASIAEAMD
                     LLEAEEPDMMLIDISLGEESGLELVKNVKALKPHIMMLVISMYDEVIYASTSLKAGAR
                     GYLMKQEAAKTLMGAIEEILKGKIYLSDAMRERMIDTMVAQQSDTPLDPIDTLSAREK
                     EVFRLIGNGYGVSEIAEMLNLSFKTINVYRDNIRRKIAVSDAGELRKYAIKWTKSREL
                     "
     gene            complement(30397..32724)
                     /locus_tag="PLF67_03465"
     CDS             complement(30397..32724)
                     /locus_tag="PLF67_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE89107.1"
                     /translation="MSKKRIGIFTASLDDEYQSTLWSAISHAIVKRGFSSISFLGSVL
                     GSPIASEASSNIAYQLANDQNIDGLIIISSSVATFLNSKDLREYFSAWANIPRVSIGM
                     KIPGMSDVTVDSEPSVSELVQHLYTVHNRRKFAMIDGPAIHDEAINRKQTVFDTLTKL
                     GIGIDPRLRVHGRFTLDSGEQAMETILATGLPFDAVICLNDRMAQGALNVLAKHSINV
                     PDDVSVVGFDGIEASRYTQPPLTTVIQPMHNLGTNAVAILERMIDGGSEEHVVLSSAV
                     TYRESCGCAPTFRFDSTVTRIPSYATQGEERTINEFAALLRMKNPKGLITQLNRAIDA
                     TIAESGVVERWHVYLAVIENLIEAEDEKHRMMISDVIASARVFVGERGSRYQASKRVA
                     LQSSFETLRTVSAMLAGTFELEALFTNLKQGLALFGITEGYLVEFTPDEGSARLLIDL
                     SWESFEKESSPLEFEYKQLLPSVYKPSFHKGRWILMPLVHGSEPLGYLIVPSGVVSPA
                     LYDVLQEQISSSLKGTLLLEQIKSHEQTLIEQVALRTKDLIRTNKNLSNEIKRRIELE
                     QEVMEISAKTMERIGQELHDDLAQHLLGISLIAASARKSINADNEKLDSSLEQISLLL
                     TESISKLKTISRGLLPLEKDEKTFTKRIEALVADTQRYAKIDIGIDADPDFEITDGDR
                     ALHVFRIIQEALTNAVKHSKSKNVVIKLERSEDEIGRVHLQASVEDNGIGFAKNIRKS
                     ALGLRIMRSRASMANATLDIQSSAEGTRVSVCLEE"
     gene            complement(32740..33564)
                     /locus_tag="PLF67_03470"
     CDS             complement(32740..33564)
                     /locus_tag="PLF67_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE89108.1"
                     /translation="MDIKDAVFVFQMHEAAELLWHQRFHSHGYNEVELHYFVGGDGTF
                     INGNKRYSISSGSLFLTTAETTHQIEASIQNPLTYYAILLECAEHHELFNRLEKKNPF
                     HLGTGRRFFFEEIRDKALKEEQELRISAAHQILGLLYGLAAEKSNPVQQKENIIIELA
                     LDYMQNHIFDKLTLSDIASHVKRDASYFVRLFKRWMNTTPMQYYANLRLEAARAFLAN
                     TNLSVKEVAAKLQYCSEFHFSKQFKQSTGLSPTAYRSRYLQKLGPFDELHSSKDTV"
     gene            33645..35132
                     /gene="xylB"
                     /locus_tag="PLF67_03475"
     CDS             33645..35132
                     /gene="xylB"
                     /locus_tag="PLF67_03475"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOE89109.1"
                     /translation="MHIVAGLDVGTQSTKLICYDATHKKVVGKASSPHRLITGEGGTS
                     EQEAIWYLDAVRSCFKQIDPSVKEKIAALGVSGQQHGFVPVGKNGEALAPVKLWNDTS
                     TVAECETLTERLGGKDAVFKLINNEIMPAYTLAKILHLKNHRTEAYEQLAHLLLPHDW
                     VNFYLTGEYFTEAGDASGTAYYSPEKRAWEPEMLRAVDEKKHLEHMLPTLIESHEIGG
                     RLCKTAAEELGLRPGIVVSTGAGDNMASAIGTGCTSGGDLVMSMGTSGTLFGYSDTLV
                     ADRQGRLSAFCSSTGGYLPLLCTNNCTLSTEVIRNLFSRDVKTLDMLAGKAPIGSRGV
                     TVLPFFTGERVPYLPYGKGVIAHLDSQNTTEENIARAALESAVFALRGGMDTFSELGF
                     TPSRLILTGGGANSKIWRQIASDVFNLEVIVPSSTESAALGVALQALWALEGGSITEI
                     ANEHVLFDEDKMHQPDRMAVEQYAEAYKRYSTYVQALTPIFTRRI"
     gene            35132..36451
                     /gene="xylA"
                     /locus_tag="PLF67_03480"
     CDS             35132..36451
                     /gene="xylA"
                     /locus_tag="PLF67_03480"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOE89110.1"
                     /translation="MSYFVGEKEYFKGIGKIRFEGKASRNPLAFKHYNANERVGDKTM
                     AEHLRFSTAYWHSFCADGSDPFGKPTIVFPFDSSDPFAKADAAFEFFTKLGTPFYAFH
                     DVDVFNDIDDGRAYEKRYQEVADGLLARQKASGVKLLWNTSNVFSHPRYMNGAATNPD
                     FNVVARAAVQIKKSLDVNVQLGGLNYVFWGGREGYMSLLNTNMKREQEHLARMLIIAR
                     DYARSIGFTGKFLIEPKPMEPTKHQYDYDAATTIGFLRRFGLQDDFSCNIEANHATLA
                     GHTFAHDLLVSSNAGMLGSVDANQGDPQNGWDTDEFPTNLYEITMAMLIILSQGGLGL
                     GGLNFDAKIRRNSTDAEDLFLAHIGGMDAFALGLKIAHKIKEEGFFRDFVDKRYASFD
                     SGDGKRFAEGKMDLVELARIGLDAKITQKSGKQELLNNMFNSYLFDY"
     gene            36625..36843
                     /locus_tag="PLF67_03485"
     CDS             36625..36843
                     /locus_tag="PLF67_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89111.1"
                     /translation="MASFDMISKDLSSHVQYRKSENSVQDLKEIIDSAQSYAFSSANI
                     ALVFRNWLIGKRISEEELQGEMRAEYGT"
BASE COUNT         8256 a         9453 c        10365 g         8807 t
ORIGIN      
        1 agcggtgcga tctcggtttt catcatgatg gcttagcgta tactcttgct caattcttga
       61 caaccgtgag gggttctttt caagctgttg acaaaaggag agcaatcgtg tagcgttcat
      121 cttgcattga gggtgtagcg aagttggtta tcgcgacggc ctgtcacgcc gtagatcgcg
      181 ggttcgagtc ccgtcatcct cgactgttcg aaaccgcctt ggagtccagg gcggtttcgc
      241 tgtcttaagg agaagagatg agagtattgc tcgggatgag cggtggaatc gattcctccg
      301 ccgccgcgta tattttgaag aatcagggtc atgaagtaat cggggttacg atgacgatct
      361 ggtccaaccg cgctcacttg cagcgaccgg tcgattcagt ctcctgcttc gctcccgaca
      421 agagcgagga tatcgaaaga atcaaagagc tgtgcgctcg cttgggaatc gaacatcaca
      481 ccttggagct cagtgaccgc ttcgaggagg aggttctttc caacttcaag gatgagtatc
      541 ttgacggaaa aactccgaac ccctgcgtgt ggtgcaacca aaggatcaaa ttcggagcga
      601 tggtcgagta cgccaaaaag agcggccttg tcttcgacaa gttcgcaacc gggcactacg
      661 cccggattgt tcatacaggg aagcggtggg cggtcgggaa ggcgaaggat gagaagaaag
      721 atcaatcgta tttcctctat cgtctcagcc aagagcagct taagacgatt ctgtttccgc
      781 ttggagagct tgccaaggat gaagtccgag tcattctgaa ggaacacgga catgtaagcg
      841 atgatcaaga agaaagccag gacttctacg acggccctta caccgatcta ttggaagtgg
      901 aggacaagga aggggcgatc atcactcgag acggacggat tcttggaacg cataaaggaa
      961 tttggcacta tacgatcggt cagcgcagag gcctcggaat cgccgccgag cgcccgttat
     1021 atgtgttgga gctgagggct tccaccaatg aggtggtcgt cggtttcgtc gatgagaccg
     1081 ttcagcatca ggtcgaagcg accgacgttg tgtttggaag tgtcgcaatg atcgaaggcg
     1141 aggtcgaggt ggaggcgaag atcagaagcg tcggaagacc gaagaaagcg atcgcgcgtc
     1201 ggaaggaaga cgggtcgatc gtcgcctttt tccccgaagg ggtgaacgcc gcgacccccg
     1261 gccaatctct ggttctctac aaggatgacc ttgtgctcgc cggcggcatc atcacccatg
     1321 cccggtgagt tgcctccctt ttggtttgcc attatgatga agcagaagga gcagggtatg
     1381 cgcgtagtta ttcaaaaaga ttttcttgcc atgagcaaat gggcggccaa gcttatcgcc
     1441 gatcgaatca acgattttca tcccaccgaa gatcgtccgt ttgtgctcgg acttcctacc
     1501 ggatcgacgc cggtcggaac gtataaggag ctgatcgctc tgtacaagga agggtatgtc
     1561 tccttcaagc acgtaatcac cttcaacatg gatgagtacg tcgggctggg aagcgaccat
     1621 ccgcaaagct atcacacctt catgtgggag aattttttca accacataga tattaaaaaa
     1681 gagcatgtcc acatccccaa cgggatggca aaggatctgg aagaagaatc gctccgctac
     1741 gaagaagcga tcgaggcggt tggcgggatt cacctctttt tgggcggagt cggcggtgac
     1801 ggacacatcg cgttcaatga gccgtactct tcatttgaca gtcgaacccg ggtgatgacg
     1861 ttgaccgaac acacccgtca ggcgaacagc cggttttttg aaaataaaat cgatctcgtt
     1921 ccccggtacg cccttaccgt cggagtgaag acggtgatga gcgccaagga ggtgatgatt
     1981 ctcatcagcg gagcaaacaa ggctcaggcg ctccatgccg ccgttgaagg gccgattact
     2041 caggcatggc cgatctcgac cctgcagctc cacaaagagg cgatcatcgt gtgtgacgaa
     2101 gatgcgtgcg gcgagctgaa ggtttcaacg tacaattact tcaaagccct cgaaaaggcg
     2161 atggtctaac gcaagccgag ctcttttgca acctcaatcg ccagctgcgg gtggtcggtc
     2221 atgatcgaat cgacccccat gacgagcaat cgtttcatct cttccttctc gttgatcgtc
     2281 cacacctgga tgatcgctcc ccgtttgtgc atgatctcca cgaagcgggg agtcaccacc
     2341 tcgattcccc attgacgggg cggaacctgg aagatcgtga gtcctttttg tttctttgat
     2401 gaaagaagtc ccagcttctg cttcgccaaa agcgggatta cctccaaggt cgtcagactg
     2461 gtttgaattc gcttctcttt gcttcgaagc gcttgaaggt ttttcaaatg gaaggaggcg
     2521 ccgatcaccc gatcgaaggc atcctttttt ctgatgagat cgatgaaggc atccacgatc
     2581 tccggttcct tgctcttcaa gtcgatgttg aaccgcgtgg cgggaaatgt ttcaagcgct
     2641 tctttgagag tggcgaggcg aacccccttg tttctgaacg ggaatgtctt gccgccatcg
     2701 acggtgaacg tgtaccccgc gtcaaaagtt ttcaactcat caagggtgtg atcctcgatt
     2761 cgtccggagg catcggtgtt tcgatccagc gtcggatcat gccagatgac gatgtgtcca
     2821 tccttgctga tgtgaacatc cgtttcgatc acatcgacct gcatggcgac cgccgattcg
     2881 aacgcaggca gggtgttttc agggaaggag ccgctgtccc cccggtgggc gacgattcgg
     2941 ggcatcggat caaagaacgg gtgggtcttg ctcatcgggc ttctccttct tatggatttt
     3001 gatccgaacc atcttggtaa agagtttttc catccgttgc acttcactct cgctgagcga
     3061 ggcgcgagtg aacacatctt tcagaaaccg tctcgtccag agctcctcct gatcgagctt
     3121 gaaatacccg atctcggaaa gggcgtccac cgccgcatcg cttgctcttt gggctcgctc
     3181 ctgagtgatc gggataagac cggtggggtg gggaaggtgt gcttcaaaga gggtataggt
     3241 gatgatttgg accgcttggg cgaggttgag ggatgggaac gcatcactgg tgggaatcga
     3301 taccacctga gcgcaggcgg cgacctcctc atcggtcaac ccgtccgact cccgtccgaa
     3361 gacgatcgag accgtgccct cggtatgcgc aattttttcg gcgagttgca gaggggagag
     3421 aaaggtgtgc tttcgaaatt tccctcttct tctggtggca gccacggtgt agctgctctc
     3481 tttgagcgct tcttggagcg ttggatgttg aactcgattc tcccagacgt cgccggcatg
     3541 aagagccagg gttcgaatcc ggttttcttc gtactcccgt tcgccaacga tgaccaggcg
     3601 ggttattccc atcgtcttca tcgcccggca gacggaaccg atgtttgctc catcctgggt
     3661 gtcgacaaga acgatttgaa tgtgatcgag tctgcttggg tgcgtcatag ctcaggtgta
     3721 ccagatattg caggattatc caagctggtc tatagtatgc ccatgaatga atccacctcg
     3781 gtaggtaatc gctatatcca tatcatgctc gggattatcg taacgttggc ggttttggct
     3841 gcgctcaaga tctccaagga tgtgacgatt ccacttgtgt tggcattctt tttctttctg
     3901 atgttcagcc cgcttttgcg gcggctggac aagctccacg tcccgaagct catttcaacg
     3961 attttcgtga tggctctgat gcttctcctt ctgttggcgg cgggatggtt cattgtcaag
     4021 acggttgaca cccttgtccg attgatcccc ttttacaccg acaagatcat cagtctcgac
     4081 cgcctcttga ccgatatggt gaacaacttc gtcgaggtac ccccttcgac gagttttctc
     4141 accttgctgc cggtcaactg gtcgagcttg gcgatcagca gcctgacgca gatctccaac
     4201 aaattcttct caatcacaaa attggcgctt ctggtctata tcttctttct gtttttgctt
     4261 ctcgagcggc aaagcgtggt tcccaagctt ctggcggcgg ttccccaagg caaggggatg
     4321 aaggtggcgg tgatgtttga acggatcacc cgccagattt ccaaatatct ggtgataaag
     4381 atcgtcatca gtttcttcac cgggttgttt ttctaccttt ccgctctcct caccgggttg
     4441 gatctcgcct cgctgtgggg agttctggcg ttcatcttca acttcattcc ttcgattgga
     4501 agcatcgtga tcacctccgt ggtgatcttc atggcgttgg ttcagttcgc tccggattgg
     4561 acgaacatca tcctggtcgc cgtacttacg atcagcaccc agatgattct gggcaatatc
     4621 atcgatcccc gcctccaagg cggacagctg aacctctccc cgttcatgat cctcgttgcc
     4681 ctttcactgt gggggtatat ctgggggatc gtcgggatgt tcatcgccgt tccgctgacc
     4741 agcgtgatgc agattctttt cgccaatatc aagagcctga ggccctttgc gatcatgatc
     4801 agcagtggaa aaagctacca acgggagcgg gcccgccaga aagcccttaa gagctatcaa
     4861 cggcggaccc agcaccggaa atctgaacag cctgccggcg agcagggcaa tgaatcgagc
     4921 gaggactcga tggcgaaaga atcggtcaat atgggtgact ttgtccttcc ggagcagttt
     4981 ggcgaaaaga aatgattgaa tttccttcgc ttatggaaga ggatgcccga agatatctcc
     5041 tgccatccaa cccctcttat gatgcattga ttgaaacagt cttgaccttc taccgggatt
     5101 ggggacgccg gttcgtcttc cgcgatcgtc acgatccgta ttcggtgctc cttggcgagg
     5161 tgatgcttca gcagacccag acatcccggg tggtcgacaa ataccggtat ttccttgccg
     5221 aatggccctc tttcaaagcc ctcgcccgcg ctccgcttga ggagatcctc atcgcttgga
     5281 agggcctcgg atacaaccgg cgggcgttga atctgaagaa agccgccgag aagaccgaag
     5341 catggaattg gacgattccc aacgatagca aggcgatgct cacccttccc ggggtgggaa
     5401 aagccaccgc agcggccctc ttggccttct gctaccaaga aaaatcaatc tatctggaaa
     5461 cgaatattcg acgggttctg ctgcattact atttccccga tcagactgat gtccatgacc
     5521 gggcgttgga agcgatcctc agtgagctca ttgtgctcat tgatgatccc cgctcctggt
     5581 actacgcgct gatggattac ggggtgctgc tcaaagccct ggttccgaat cccaacacca
     5641 aaagcgccca ctatgcgaaa caaagccggt ttgaagggtc caagcgccaa gtccgggcgg
     5701 cacttcttca ttgtgtcgcc gaacaggggc cgatcgctct ttcatccgtc accgagatct
     5761 tgaaagagta tgattcaaca taccttgctc aatctgttga ggagctcatc aaggaagggt
     5821 ttctccttct tgagaaggat tctctttcga tccggtagaa cgatcggcaa gcagattgcc
     5881 cgccatcccc aggagcatca ggagcgctcc gaagaggagg ttgggggaga agatcttcag
     5941 gaagaacgca tcgatcgcca ccgatgcgag aatctgactc gccgagagaa gcaaggcgga
     6001 gtagatcgcc gggagcttca cgatgacgaa cgccgtggag atcaggacgg tcaccgagag
     6061 gacgccgcct gcgatgattt ggatgaacgg cacgctcggc aatctggcga atccttcgat
     6121 cgtcgccgaa ggccgggtga gaaagtagaa gggaaccgcg gcgctcatgc cggcaatgta
     6181 ggtccattgg ctggctttca tcgggccttt atagattgcg aaggtggagt tgaggatcat
     6241 ctgggtgatc gtgatcgcac cggcggcgaa ggcgatgagg atgtagatcc atgaaaacga
     6301 gccgacgccc ttggaggcca tcacgacgat tccaaggccg ctgaccgcca gactcagcac
     6361 tttcttcggg gagaaagagc gcttcttcat ccccaaccat ccgttgaggt caaatacaag
     6421 ggaaagaacc gattgaccga agacggtgag cgccatcgcc aaggaggaac cgagggcggt
     6481 gaaggaatga aagttgaagt tgagaatgag gacgccgagc atcccgttga ggtagagagg
     6541 ccagggggct cgtcgtttgg gcccggcgat cgcggggttg ttccgcccga agaagaggat
     6601 cgtcgagacg agggtgaagc cgatgagatg attcaccagg aggctcactc ccatcgtcgt
     6661 ggcatgcccc aggttggtgt tcgccaccac catgacggag atgatcgcac cggtcagccc
     6721 gtcgagggcg aggtagattc cttgattcat acggtgagtg tacttcgtta cggcttgtca
     6781 tgctagtggg cccgctctac aatgatgtcc atggattacc ggaagctcct cctcaaaaaa
     6841 gcgatgatac ccggctctct gggctttctc ggcgaccttg ccctgcagct tgccaagttt
     6901 tatcctcgtc cccttgatac gttctccctt ctgctctttg ccggagacca tgggatccac
     6961 ggtgaaggag ttacctacag tccgcaggag atcacctggc agcagacccg cagcttcgct
     7021 tccggcgggg gagcgtgcac cctctttgcc aagctgaacg gaatcgacct caaaataatc
     7081 gatgtcgggg tcgccggatc gttcgatcct tccacgtcgg tgatcgaccg gaaggtgggg
     7141 ttcggaacga agaactttct gcatgaacag gcgatgaccg acaaggaatg cgcctccgcc
     7201 atcgaggtcg gcagagcgat gagccgttca tccggtgccg atctgatcgg cttcggcgag
     7261 atgggggtgg cgaacaccac ctcttcatcg gcgatcgtcg ctgcattgtt ggatcggaag
     7321 cccgaggagg tgacggacgg cgggtcgggg ctcagcgaac cggaccttcg ccataagatc
     7381 gaggtcattc gaaaaggtct tgccttccac ccccgacggg atcctttgga tgttctttcc
     7441 gctttcggcg ggtttgaaca tgcggcgatc gtcggaggaa tcctggaggc atatacgatg
     7501 aagaagccga tcctccttga cgggttcgtc gtgggcgccg ctgcattggt tgcagcgatg
     7561 attgatccga atgttaaaca gaacttcatc ttcgcccacc gttcggggat gaagggcagc
     7621 ggcatccttt tggaagccct cggctgcaca aagccgatcc tggatttcgg aatgtacctc
     7681 ggcgagggga cgggggcgct gactgcgtgg ccgatcgttc gccaagccag ccacatcctc
     7741 tgggagatga gcgagttcga gcaggcggag gtgaccgatt ccaccgccat tctccggtcg
     7801 aagggtcttc tatgatccga ctcggaacca ccagctacat catcgaggat acgctgggcg
     7861 ggaatgtccg gtatctcgct ccgatggtgg acgatgtcga gcttgtgttg tttgaaactc
     7921 ccgaggcttc aaatattccg aacaccgccg agatcgaccg gttgaatggg ttggcatctg
     7981 aatatgatct tacctatacg atccatctgc cggtggatgc ccgtccgggc tcacgggatg
     8041 aaaaggtgcg cgcctcttcg gtcgatctga tcggccgggt cattgaacaa acccgggttt
     8101 tgaagccctt cgggtacatc cttcatctcg ctcccgagcg gtacgggccg attcccgccg
     8161 aggatgtgcg cacctggctg attcagagtg agaaaagcgt catcgatctg ctgcaaagaa
     8221 cgggagtcaa tcccctcctg ctgggcgtcg agacgctggg ctatccgctt gatctggtcg
     8281 attccatcat cagtcgccat gggttgggtg taacgttgga tataggacac ctgcatctga
     8341 tggggtatga tgtggaagac catgtgaagc gctatgcaag ccggtgccgg gtgtttcacc
     8401 ttcatggagt gaacgagggg acggatcatc tcggtcttca tcggggcgat cgggagaaga
     8461 tcgatcgatt cttggatctg cttgcttcgt tggaagatgg gatcgaacgg gtggtcactc
     8521 ttgaagtgtt tgaccgggaa agttttgaag gatcgatgga ggtgctccat gggcgaaacg
     8581 gaggagagaa agatggttac cttggtgatc gggggcggac gaagcgggaa aagcacgtgg
     8641 gcggaagcat acgctcgatc ccttggcggg aagcgggtct atctggcgac cgccgaagcg
     8701 tttgatgagg agatgaaaga gcgaatcagg gctcatcaag ctcgccgaag cgggcagttc
     8761 atcacgattg aagagcctct tcagctgagc gggcggatca tgaaccttcc ctccgatgtc
     8821 cgggtgtgcc tcgtcgattg ccttaccgtc tggctgggaa acctgatgca ttacgaaaag
     8881 gatcttgaag catatcttga ggagctgtac cgggtggtgg atgatccccc ctgcgagctg
     8941 attctggtga ccaatgaaac ggggttgggg atcgttcccg ccgatccgat gagccggatt
     9001 tttcgtgacc gggcggggtg gatgaatcaa cggcttgctt tgttggcgga tcgggtggtt
     9061 ctcctcgtgg cgggcattcc cttggccatc aaaggaacac tgccatgagc ctggcaagcg
     9121 cgcttcgtct tcttacccga ttccgcatcc ccggcaagga gatcgagcag agcgggcgga
     9181 ccctcttctg gtttcccatt gtgggggctc ttctgggttg tatctcctgc ctttttgccc
     9241 tgcttccact ttccgccccg attcgcagcg ttctcgtcct catcggcgga gtgtatttca
     9301 cccgggccct tcactatgac ggcgtggcgg atcttgccga cggactgggc ggcggcttta
     9361 caaaagaacg atccttggaa atcatgcggg acagccatat cggggcgttc ggcacgatcg
     9421 ctttgatgct cgttgttttg ctgcagtatt cgcttctcct cgacgtggtc gaacatcccc
     9481 ccatgctgat ccttgtttcc actttgggcc ggttggcgca ggtgtgggcg gcttcactga
     9541 tgaactacgc ccgaaaaggg gaggggatgg cttcttcgtt ggtgcgcagg gcgaagtggc
     9601 atcatgccct tcttcccacc ctccaagtcg gtctgcttat cctgctcctg tttgttttac
     9661 atcatcccta tgcgatcccg gttcttgccg cttttggagg gatcgtcctc agcacgacga
     9721 tcctctgtct gatttcccga aagcgtttgg ggggaatcac cggcgatgtg ctgggaagcg
     9781 tcgaggtgtt cggtgaaacc gccgccctcc tcggaatcgt gttcgctctt gcataggtga
     9841 acactctttg caatactaaa gccaattgga gcatatggta tgaagattgt ctgtttggat
     9901 ttggaagggg tgctggtgcc ggagatctgg atcaacgtgg caaagcgaac gggaatcgaa
     9961 gagctgcgcc ggacgacccg ggatgagccg gattacaaca agctgatgcg ccgccgaatc
    10021 gaaatcctca aggagcattc gttgacgctt tccgatattc aggcggtcat cgcctcgatg
    10081 ggaccgatgg aaggggcgaa agcgtttctg gatgagctga ggtcgttgac ccaggtgatc
    10141 atcctcagcg acacgtttgt ggagtttgcc aaaccgctca tggcccggct ggattggccg
    10201 acgatctggt gcaacagtct ggaagtgaaa gacggaatga ttacccgtca caagttgcgc
    10261 caagaggagg gaaagttcca tgcagtgcag gccctcaaag cactgaactt ctcgacgttc
    10321 gccgccgggg actcctacaa cgacttgaag atgatcttgg aagcggatga aggatgtctc
    10381 ttccgagcac cgaagacgat catcgaaagc tacccccatc tcgttctcac cacaacgtac
    10441 ggtgagctga tggatcggat caaggagttt ttgacatcgt gatcttgcga ccggtcatct
    10501 ttctggtatt ggcgatctta gccggctttt ctctcctcgg ggtgttgtgg gagggttcag
    10561 tcttcatcaa ggttgtggta ggattgacag tagccgcttt tagtgtcgga ttactgatcg
    10621 tggcaagaag gttcgatcgg gcgctcaaag aaaagagggg agatgcggac gatgcggaaa
    10681 acggtggtat ttctcagtga tttcggcagt agcgacggag cggtcagcgc aatgcacggt
    10741 gttgccgatc aggtctcggc ggatctcaaa ctggaagatt tgacccacga tatccctcaa
    10801 ttcaacattt gggaggcttc ctatcgctta gcccagaccc ttccgtattg ggcgccgggg
    10861 acggtcttcg tctgcgtcgt cgatccggga gtcggatctg atcggaaatc cctcgtcgcg
    10921 ttgacaaatg acggacacat cgtcgtcact cccgataacg ggacgcttac gcacatcgaa
    10981 cggcggatcg gactgaagga ggtgcgggag ctgcacgtcg aaacaaaccg cttggcgggc
    11041 agccaaaaga gccatacgtt cttcggacgg gatgtgtatg cgtacaacgg agcacggctc
    11101 gcctcgggcg agctcgcctt cagggattgc gggccggttc ttgaagagtt catccggctc
    11161 gaagtggaag agcagcgccg cgagggggag acgatcgtcg gagcgatcga tattctcgat
    11221 acgcggtacg gatcgctttg gaccaatatc aacggagact tgttggacga gatcggcgtc
    11281 acatggggcg attatctcca cgtgcggatc accaaggaca accgaatcgt ctatgggtat
    11341 ccgctcaggc tgtgcaaggc cttcaccgac gtacccaagg gcgaagcgtt gatgtacatc
    11401 aacagcctct tgaatattgc agttgcgttg aatcagggaa atttcgcctc tacctattcg
    11461 atcggaacgg gtgaggggtg gaagataacg ttttcgaaat acgatacgcc ataaggagga
    11521 atcaagatgg caaaagcaaa aggtttggat ccggtacgat ttgtcgtaat cgtggcgatt
    11581 ggggcggccc tctacggtct gggtgggatg attggggtgc cgatcttcgc caacaccacc
    11641 ttaaagccgg cgatggcgat tttgggactt tttgccgcac tctacggacc gattgtcgga
    11701 tttttggtag gattcctcgg tcactggatc accgacctct tcatggggtg gggcgtatgg
    11761 ttcacgtggg tcctcggaag tggtcttgtc ggagtcatga tcgggctttt caagtattgg
    11821 agcaaggatt gcgtgcatga agggaatctt cccggaatgc agattcttct cttcattgtg
    11881 atttccttcg ttgcaaactt cctcggctat ttggtcagtg ccttgcttga tgcgttgctg
    11941 tataccgagc cgttggataa ggtctttact caacaaattc ttgcggcgac caccaacacg
    12001 ttgatgatcg cgacacttgg aacccttctg ctctacctcg ttgcccgccg caacgccagc
    12061 agtcgggatc tgaaaaaaga cgaaaaagcg taagaagctc acatgtgcct gcaccatgca
    12121 gtgcaggctt tcgccatggc cgaagttctc atctccttca aggagttttc ctttacctat
    12181 aaggctcaaa gcgagccgac gctcaagcat atcaatctct cgattcaccg gggagaaaaa
    12241 atccttatcg ccggaccttc cgggagcggc aaatccaccc tcggctactg catcaacgcc
    12301 ctcattcccc acgcgttcaa gggaaaaatt gaaggaaacg ccacgatatg cgggttgaat
    12361 cttgcagatg gggatatgta cgcggtgaac aaacgcgtcg gaacggtgat gcaggatacc
    12421 gatgcgcagt tcgtgggaat aaccgtcgct gaagacattg cgttttcttt ggaaaaccaa
    12481 tgcgtcgaac aacgggagat gcactccacg gttgctgaaa tcgcccgcgt ggtgaagatg
    12541 ggatcgttcc tttcccattc gccccaagag ctttccggcg gacagaaaca gcgggtgagc
    12601 ctggcggggg tgttggtcga tgatgtggac atcctcctct tcgacgagcc gctggcgaac
    12661 ttggatcccg cgacgggggc cctcgcgatc gagatgatcg acgagttggc caacctccat
    12721 aacaagaccg tcatcatcat cgaacatcga ttggaggagg ttctgcaccg gaaggtggat
    12781 cgaatcctgg tgatgcagga aggaaagctg gtcagcgata cgagacccaa ggagctcctc
    12841 gcatctcaaa cgctgcgcgc cctcggcctc cgcgagccgc tctacatcac ggcgctgagg
    12901 atggccgggt gcgatctgaa gagggcgggc gacctgagct cctatgaagg agtgaagctc
    12961 gattccgtca aggaggcgat tcatgattgg tatgcggttt cacagaagga ctcagcggct
    13021 cgagagcggg atgtgcttct caggctggaa aacttgagct actcatacga cggtctggtc
    13081 atggcggtgg agggaatcag cgcatcgatc cattcaggcg agatgatttc ggttctgggg
    13141 aacaacgggg cgggcaagtc caccttggct cagctgatca tgggcgtgtt acgccaagac
    13201 agcggatcga tatcgtttga agggattgac ctcgccgggt actcggccgg cgcccgctcc
    13261 gatctggtcg ggtttgtgat gcagaacccc aaccacatga tcagcaacga tctggtgttc
    13321 gatgaagtgg cgttcgccct ccggcaacga ggctatgagg agaccctgat cagggagcgg
    13381 gtccttgatg tgctggggct ctgtggattg cgtgagtatc accactggcc gatcagtgcg
    13441 ctcagctatg gccagaagaa gcgggttacg atcgcctcga ttctggtcac cgatccgaaa
    13501 atcctgctgc tcgatgaacc gaccagcgct caggattacc ttcactacac cgctttgatg
    13561 cagttccttg ccgagctgaa tgagaagacc gggcttacga tcctcttcat cacccacgat
    13621 atgcatttgg cgctggagta tacgacccgc tcgctcgtgc tcagcggggg aaagctcctt
    13681 gccgatctgc ccaccagcga ggtgttcagc aatccggatc ttctttcccg ggcgaatctc
    13741 aaagaaacaa gtctttatta cttggccaaa gatctgggaa tcgccgatat tccgcttttc
    13801 atcgaacatt tcgtgcagga ggaacggaaa gaacgtcccc cgagcgtcga ggtcatccgg
    13861 ttgccgaagc ggaagcttgc cctcaccgag cgaaaaaaag agataaaggt cggcaagaag
    13921 gtcgtcaagg aggggcggaa gttcggtttc gctctcagct gggaggacac cgattcctac
    13981 cttcattcct tcaacggagt aacgaagatc gcctttttcg gactttggat cgggttctgc
    14041 ctgctgacgt tcgatcttcg gtttcttctg ccggcccttg gcttttcctt ctacctgctg
    14101 tgggcgacca aggttccttt gcgaaagttc cttccgtaca tcatcggaat cctcggcgtc
    14161 atcgtcctca acgcgatctt catcttcctc ttctctccga agcagggggt ggtgtacctc
    14221 ggttcggaga cccttcttct gggaaacgag atgatgcgct acgccctcag cagggaaacc
    14281 ctcttctacc tgttcgtcgt atgcgtcaaa tatcttacgc tctttcccgt ggcgctcgtc
    14341 ttcgtctcga tcacccatcc ttcggagttc gcctcctccc tgaaccggtt ggggatcgga
    14401 tatacggtga gctacgccgt ttcgctggcg cttcgatatc tgcccgaggt aaccaagacg
    14461 tatgtgaaca tccttcgctc tcaaatggct cggggggtgg atatctccaa aaatgcaccg
    14521 ttgagaaaac gtctttcttc aatggccagg atcatcgccc cgttggttct cttcagtttg
    14581 gatcggatcg aagtgattac caatgcgatg gtcctccgcg ggtttgcccg gctgaagaaa
    14641 cggacgtggt acatgcagag ctcccttcgc ccccgggatt gggtcgccct tggacttacc
    14701 ctcctttttg tcgccgcttc tctctacatt cgatttgcac ggaacgtcat gttctggtat
    14761 cctttctaga caattgtcct gtttagcatg aaaagttgag ggcagagctt gacagaagcg
    14821 cctcttacac ccatcttata ggacattgat ttcattggtg tggaaacatt ggcgaaggag
    14881 gaagtaatgg ctgatacatt cattgatgaa atggaagaac tgctcacaac gatgcgcaaa
    14941 gagctgcttg aaaagatgag tgaagataac agcgatttca aatcgatggt caatgcgatg
    15001 ggcggcaaag acagcgtcga cattgctgcc gatgacatcg cttttaaaaa gatggaagcg
    15061 atcaacaagc atgaagccaa tcgccttcgc tcaatcgata acgccctttc acggattcat
    15121 aacggaaagt atggactgtg tctgaaatgc caaaagaaga tccccgaaga acgccttcgg
    15181 gcgattccat acgcggttct ttgcgtcgat tgcaagctcg ccgaggagaa acccggtcga
    15241 cgatgatatc ggagcgtacc gagcagcaga ggctacctac gcaggtagcc tctcgtcgtc
    15301 tttacaggta cgggtttttt cgtcgttcat caccgactgt cgttgtcggt ccgtgaccgg
    15361 ggagaatccg tacatcatcc tccagctcca gaatccgata acagcttttg atcagggtgg
    15421 ggtagtcccc gcccggcaag tccgttcgcc cgatcgctct ggcgaagagg gtgtctccgg
    15481 tgaagatgaa ccgcccttcc ctgtggtagt aggagcgccc accgggggta tggccgggcg
    15541 tggcgatgac ggacagccgc gaatcctcaa ggtattgtcc gtcgataaga tagtcattgg
    15601 gcttggggag gtgtttgagc gcttcttcat gaatggtgaa gaagcggcga tcaaggatgg
    15661 ttgctttgat gacttcataa tcgaggcctt tcgcgtcctc cttgcccaga agaacttcca
    15721 tatccggata gtgctccttg aggcgggtca acgcagcgat atggtcatag tgggtatgcg
    15781 tcaacagagc gctccgggca gtaagaccca acgaggtcaa ttcatcgata atcagctctc
    15841 catcggttcc cggatcgatt atccaggcac tcttcgtctc ttcactcccg aggatgtagc
    15901 agtttgtctg atattcacca acaaccaatc gtctgagaat catcactact cccggttgga
    15961 ataggagact gcagcggttc tgcccttgat atccattccg ttgagttttg aaatcgcctc
    16021 ttcgcaatgc tgttcgctca tcgagatgaa cgagtacttg tcatgaattc gcaagctata
    16081 gatatcttcc cgcttgattc caagctcccg ctggagcagc tcactcagct ctttggcgta
    16141 gaggcgtttc atcttgccga tattcagata aagggtccgg gcgccttcgg gaaggggttg
    16201 attctgactt cctcgccgag gctggggagg ggcgctcttt tggggcgcgg gggctttctt
    16261 cggggcgggg gcttttttgg gggtgttggc tttgagaatc tcccggagca ggtatgcagt
    16321 gaagtatctg cgggtaaaga ggggaacctg ctttttgatg gttttcgcca gcgcttcgag
    16381 ctcctccggt tcagagtcgg cgtggacttt cgtgacaagc aattggattg tggattcaat
    16441 caattcctta tcttcctgtg tgctgtttgt ttcgttgctc atgcacatcg ctccttggat
    16501 aaaaatgttc gccttcagga aagaaggcta aaaaaagggg atgagcagta cacgaaaaaa
    16561 agctctcccg cttcatgaat tttcgcctta cacaaaagaa aataaggtcg aaataggtgt
    16621 attcaaggat actcaacctc atcttccttt gtcaaggaac gcttactttg ccgatgcact
    16681 ttgattgtgg tattatgact cggaatgagt acgttgcaaa gccagattca gcgcctctcc
    16741 cgtgcgatcg aagagcatcg gcgggatctt cgcacgctct atggtgattt gggaaaagct
    16801 acggcaacgg acgatagccc ttccgctcag gaggttctgc aaaagaaacg ggagtacgag
    16861 attcaggagg aactctatca gcagctgact ttttcactga aacagtttga agaccggagc
    16921 cgcaagctga agcaggttca gaaagatctt aaagttcttt cgaaggagaa aaaagagctt
    16981 tgcgcgcagc ttggcgcgat tgcctacgaa gcatatggaa acgcatccta ctccgatgag
    17041 ctttccaaaa cccttgcccc gtttctagcg gtcagaaaac ggagaaatgc cggagctccc
    17101 gtctttgccc acttgcaccg gcgagcactc ggacggatgt ttctgaaatt gggggaagaa
    17161 atcctcacca ggcatcttga ggaggagctt cgaagtgaag ctcgcgagga gctgttcaaa
    17221 caggtccggg agtatcgaag caaggaacac cttctgggcg aggagctcgc ccttcaccaa
    17281 agcgcccttg atcagctgaa gaacagcgag gttccaacgc cttggctgcg tcttgaaaca
    17341 tacaaccaaa gtcggatgaa agctctgaag gcgtatgaag aagcagcgat cgcctatggg
    17401 cgacagatgt atgaacacga aggcgatcaa aaccctctgg caacgcaaat cactttgcat
    17461 cgaactcgaa tcaagcagtt aggcggtgaa atcaaggaaa aacaaaaccg gatcaaaatt
    17521 caggagcttg aggctcagat tgagatcgaa aagcagaaga ttgagcatat cgccgaccag
    17581 atcggagccc tccgcgccca aatcgaccag ctcaactccg ccatcgccaa acgacggacc
    17641 ttgatccgca tgctcgaagg aaccggcaac gatggctgat tcgaagaact ccaacgtcac
    17701 tacgcgctca atgaaagtaa ccatagggga gaagaagtct caggttccgt ctaagaaacg
    17761 gaatgatgaa gagaaggaac ccgtaaaacc tcatccgccg attaagaata agagtgaagg
    17821 gaaggtcgcg ttgcgaaagc cgatcgttcc gcctccgatt gggaagcgca aaggataccg
    17881 taggccgatg aaggagcttc tgctcatctc tttattggcg atcgttatcc ttgccgggat
    17941 gggagcggtg gggtggaaca tctcgcgcaa ggctccattt gatgatgaag tagtcgctgt
    18001 cacgatcgag gcgggaatga gtgcgaccga ggtaagcttt atacttgagg aagccgggat
    18061 catcgatgac cggaagcgct ttctcgatta tgtcgtcagc cggcaagccg cctccctgct
    18121 caagagcggc acctatatcc tcggaaagaa ctcaacccat gaagagattc tcaaagcgct
    18181 ttcctctacc ggtcgggttc tggagctgct cgtctatccc gggtataccc tgaagcagat
    18241 cgatgagatg ttgaccgttc ggacgggaag caaaccgggg gctttcgtga aggccgcctg
    18301 ggaggtcggt tcggcgtatg ggctgaggtt tgtcgaaggg tggttcctgg ggggaaccta
    18361 tccggtcgag ataggcgaac gaagcgcccg ggatctcgct ctttcgatgt tcttcgccac
    18421 cgaggaggtc gttcggacgt tgagcgattc acctctgttg gatcgatatt ccatcgaaga
    18481 gctgatcatc atcgcctcaa tgatccaagc ggagactcag gaccaaagcc aaatgggagg
    18541 tatcgcctcg gtgatccaca atcgtcttga gaagaacgag ccgctcggaa tcgatgcgac
    18601 gacccggtat gagttggatg attgggaaaa cccgattccc gtcgaggctc tggaacagaa
    18661 gaccccgtac aacacccgca gaaagacggg cctgccccca actggcatcg ccgccccctc
    18721 caaagcggct ctgcatgccg cattctatcc ggagacgacc ccatatttct actatctcca
    18781 tggtttcgat aagcggattc actacgccgt cgattacgaa gaacacaagc aaaacatcga
    18841 gaagtatcgg aaatgagaac aaggttgcct gagcaacctt gtttctaccg gcggtcggag
    18901 tcgaaccgac acggattatt catccatcag attttgagtc tgacgtgtat accaatttca
    18961 ccacgccggc gcggtgagaa ttatgctacc aaaaggatgg taaaaaagca acagcgatag
    19021 tggaatcact caattgtttg ttgttatttg aatcttgatg gggtaaggtt tcctcacgga
    19081 ggagatacat gctcaagaag attcttgcaa tcatctttgt tttactattt accatcggta
    19141 tctctgcgaa agacttgatg cttgccgatc tgccgatctg gtatgggaag gagcggttcg
    19201 tcgagcggat cgaagagcga accgggggga ttcgtgaacc gatcggtctg gttctcagcg
    19261 gcggttcggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gattcgttca
    19381 tctggatccg aaacgacgtc gagtcgatct ccttcatgga gtttgccaaa gttctgtatc
    19441 ttcaggagaa ggggtatgaa gcggcacagg cgaacggcga agtgctgggg cggctgaaga
    19501 ggggaatttc cgtatcctcg tttgccgaaa aaagagaacg gcttgatgag gcgatccggg
    19561 ccgccgatcg gcaatacgcc gcctttgaac acctcaggcg gtttgatgat cctcagctgc
    19621 tgggaatcgg actgtggtcc accatcaccg gcggggtgcg gccatacctc cttgatgatt
    19681 tcaccatggg aatctcctat acggcgtcca gacaggcgtt gagcttcgag ctgaacgggg
    19741 gctttgcgtt caactgggcc cgaagtggac agatgcgcac cgtccccgcg atcaagactc
    19801 gcttgtacta ctactttctc gaccattggc gggtcgcttt cgggggcggg gtcttctatg
    19861 acgcgaggaa agagggattc ctgttttcct acgatgggct gctcgagggg cggtattcgc
    19921 ttggcaggat cgccgatctg aggataagcc agacgttcga agggctcttc aacgacagga
    19981 acagcaggta cgtggaggcg tttcccggat caacctgtct ggcgacgagc accgtcacgc
    20041 tcgaactgcc aagcttgaaa tggctggaga aaggctcggt ggatctcgcc tatcaagcgt
    20101 atggggatta tcagcggatt cggagctttc tcaacccgta cgggagtgtg aaaatccgcg
    20161 atccgtttat cggaatctat cttgaaggga agaccagcgt ccggttcgcg ttggatggca
    20221 agggcgatgt accgctcttc atctccgatc ggtttcgaac gaaccacgat ccgctcataa
    20281 tccaagggca cgatcttacc gtttcaagca atccttcgaa gtatctggcc gtggtgaacc
    20341 tcggctttgg gtatcagccg gtgtcctatc ggccgacctt cggcgagatg ctcctcttcg
    20401 aggacagcag agtaggagta tttgccgacc tgctcttcaa cggtgatttc ttcttctcgg
    20461 tgggcctgga ggccgaaacg acgatcagcc tgctcggcat caccgaattg gggattcgat
    20521 tctatggtgg atgggatcag gcaagccgga atccgatttg gggcgtatac ttcactgttc
    20581 ctcattagct tcgatatagg caagatcctc ggcagtgagc tccaagtttg gaccctgttc
    20641 ttcaggctct actttttgct tttttcgtcc cgccggtcgt ttggcaaatc cggaaaggat
    20701 caagccttct tccggttcaa aggtccggtc gattgctttg acctcttgac cggaggcaac
    20761 ggcgaggcgg tcctgatcga tttccatggc ggtttttctc cgggttttcg ggtcgctcaa
    20821 tcggtcatgg agatcttgga tataggaagc ttttatcccg gaggtattga cgaacggttt
    20881 ttttaatcct tcggcgagaa gatcgatcgc ttcaccgatg aggccccagt ggatttttac
    20941 ctgggccagg tggaggtagg gttcggcgaa gcgaggacct ttggagatca gatcggtgag
    21001 aatcagttcc gcctcaaccc actcattttt gctgatcgcc agccacgccc ggttatcgat
    21061 cagtccggcg ctgacgatat ttttctcatc cgcttcatca aggaggtttt ccgcttcctt
    21121 ccatttacgc tccaccaagg cgaaggtggt gtagttgacc aacagattgc tgtctcggaa
    21181 gcccgcctca tagacctccc gaacggtctt tagagcggct tttgtatctc cttcgatcca
    21241 gtgcaccatc gaatcgagaa tgccgacgat tgcatcaagc tccttggttt gtcccttcgt
    21301 gaggtttttg tatgactcca aaatggtttt tccttcatct acatcgcccc gttggatgaa
    21361 tatgcttgca atggcgattc ggacgttggg tgcaactccg gctttcaatg cttttcgata
    21421 gagaacccat acctcatcca acgctccgct ttgaagcttt ttattcgctt ttccaaagta
    21481 caggacacct cttcgataaa agagaaggcc tgcaaaagct ccaaaaagga tgatgagctt
    21541 tgcagtccgg gaaatcggca gagcgacgat aagaatcgta cctgcgattg atatgtataa
    21601 gccaaggttc ttcttcatac gtacccccat cctctttact ctatctgaac ggtgggggtg
    21661 agacaagcac tcctcttgca gagcggatgg gcgatgttct acgataggtg caatgatgaa
    21721 acgacgtgat atcctggttc atgcatgctg tggaccatgc agtacatcaa gcattgagcg
    21781 gatgctcggg gaggggtgga atcccgtcct gtacttttcg aactccaaca tcttcccctt
    21841 cgaagaagcc caaaaacgct ttgacgagtt gatgaaggtc gcaaagatat atgagcttga
    21901 ggtaatccgg gaaatctatg atcacgaatc gtggctcgag gcgatccggg ggtttgaaaa
    21961 agaacaggaa ggcggagagc gctgcaggaa ctgcttcgcc tacaatttgg ccgaggccaa
    22021 cgccaaagcc attcaattgg gtttcgacca cttcaccacg acgctcacgg tcagccgctt
    22081 caagcattca ccgacgatct tcgctgttgg aaagcagtac gagcgctttg aagcgattga
    22141 ctttaaaaaa caggggggat ttgagcggtc aaatatcctc agcagggaat atggtctgta
    22201 ccgccagcaa tattgcggtt gtgagttcag cattcgccct tagcatgata tagtggcata
    22261 tagggaggcg cgatgagaaa gatattaaag tttcttacga gccgtctctt tatctctttc
    22321 ctcttgattt ccatccaaat cggcttcttt gtcctcattg gaatctatgc tcaatcgtat
    22381 ggggtgtggt ttcagttgct ttccgctctc agcgtgttga tgagtctgtt cgtcattacc
    22441 cgcgatttga acccggccta taagatcgga tggatgttcc ttttcatcgt cattcccatc
    22501 tatggcgggt tgttctatct cctcttcggc actcataagc tcaatcgaag attgcgggcg
    22561 cgcgtcgaag agcttgactt ctcctttcgc aagggaatga aggatgctct gtacagcgat
    22621 cttttgccga ttcgagccct ggaatcctat tccccttcgc ttgctcgtca agcgcaattt
    22681 atttgctcga tcaccgggaa tcaagtgcat gtcaacacgc aagtcgatta tttcgccagt
    22741 ggggaagcgt ggtttgatga tgtgattatc gagcttcaaa aggcaaaatc gtttattttt
    22801 ttggagtttt tcatccttgc tgaaggtgaa atgtgggacc ttctgctttc aatccttctt
    22861 gaacgaagaa agcgaggagt gcgggtatgt ttgatgtatg atgatgtggg ttcgatcttc
    22921 accaccccca gccattacga tcggacgctt cggggccttg ggttggaagt cgtcgccttc
    22981 aaccctttgc gcacccacct caacagccgc ctcaacagcc gcgatcaccg aaaggtaatc
    23041 gtcatcgacg ggaacatcgg atataccggc ggaatgaata tcgccgatga atatatcaat
    23101 cgaacgatca aatatgggca ttggaaagat acagcggtca aaatcacggg cgatgcggtc
    23161 tggtcgttga cccagatgtt ccttcagctc tggtcctttt caacgggagt caccgaacag
    23221 ttcgccctgt atcagccaac gattacggcg aagaccgacg gcttcgtcca gcctttcggg
    23281 gacaatcccc ttgatagtca caatgtagcg gagaacgcgt atatccaggt gatcaacatg
    23341 gccaaacgct acgtctatat cacgaccccc taccttgtgt tggacaatga gatgctgacc
    23401 gctttgaaga tcgccgccca aagcggagtg gatgttcgaa tcgtgactcc ctacattccc
    23461 gacaagaagg gcgtgttcct cgttacgcgg gcgaactatt acaaactcct tgaagccggg
    23521 gtgaaaatct atgaatacac tcccgggttc atccatgcca agatgttcgt cagcgacgat
    23581 catgtcgcga tcatcggtac gatcaacatg gattaccgtt cgttctatct tcacttcgaa
    23641 aatgcggtcg ttctttatgg ttcctcggcg atcaagcacg tgtatgaaga tatcgaacgt
    23701 acgatcatgt tgggtaaacc gattacgacc aagcttctca aagctcggcc ctggcatcaa
    23761 agactgggtg ggatgattct caagatcgca gctccgatgc tttgacaggg aaacacccga
    23821 catgcggtat gccgggtgtt tctgcatggg ttgctttttg cgtttatgta atcatcacat
    23881 gtgagcgatc attccatgaa ttgaaatcga ggattcgcac tccgtccttg accttcaaga
    23941 ggaaggaatg atgaatgtcc cgacttgatg aaccgatgaa gagatcgaat tcgcctcctt
    24001 caagaatccg ctttccgttc ttgtagaagg agaggagatc ggttttgaag gtaaaggtca
    24061 cggtcttctc ttctcctgct ttgagcggga ctcgagcgaa ccctttgagc tcttttaccg
    24121 gccgcaccaa ggaagcgtga cggtcgtgga tgtagagctg cacgacctct tcaccatcga
    24181 tggtacccgt attctttacc gtaacgagta cctcgacttc gccgtcaagg tctaccgtat
    24241 cagtcttgag cttcgctgcc gggtaggtaa aggaggtgta ggaaagaccg aatccgaacg
    24301 gatatcgggc accgaacgca ctttggcggg gcaggccgcc ggatttcttg ctgtgattgt
    24361 aggtatacgg catcgctccg gcactgaagg gaaaggagag ggtggtcttg ccgctgaagt
    24421 ttttctcccc gaagagagtc tgtgcaatcg cttctccacc gccttggccg ggcagccatg
    24481 ctgccaccag cgctccggcc cgtttaaccg cgctgttgac ggtgtatgga cgaccgctga
    24541 cgagaagaac gacagtgggc ttgcccgtgg cgagaatcgc ttccagcaat tcctcctgga
    24601 caccggggag cttgagggtg ttggcatcac tgccctcacc ggtcgtgccc tgctggaaga
    24661 ggccgaccag gtccccgacg acaaggacga cgacatcggc attctgagcg gtcttgaccg
    24721 catcgatgat ccggctggta tcggtcgaag gaactttcgc caagggaccg tcctgctgtt
    24781 cgacttcccc ggggaagaag atcgaccgtt cgattgagga ctcgaagatg acgcatcccg
    24841 gttggaactc gacttcggca tatgcttcga gcgcttcctt gatcgtcttg cttctctttg
    24901 gaacggtctc ttcccacttg gcggatcctt gaaggtgaac cggtgcggta tagccgccgt
    24961 acattgcata tggatgattg cccaaaggac cgatgagggc gatcttctta cctctctgca
    25021 aaggaagaag ttcttcattc ttaagcagga cgagcgactt ttgagcgatc tccacggcca
    25081 gtgcatgatg ttcatcgctg ctcagctcaa gaccttcttc atcgatgtag gggttgtcga
    25141 agattccttg acgatatttc tcggcaagaa ccgtcataac cgctcgatcg atctcgtcca
    25201 tcgtgatgag cttgcgttca aaggcggcga tcaatccttt cttgaagacg gtgaagccgg
    25261 gaagctcgat gtccattccc gccttcaagg cgagagccgc cgcttcggcc gggtcttttg
    25321 ccacgtggtg gtccttttcc agttgagcga tcgcttcata gtcggcgacg atgagcccgt
    25381 caaagcccca ctccttttta agaaggtcga cgacaagctc gacgttggag gtgcacggaa
    25441 ttccgtcgat gtcgtgatag gcgggcatca cacccccgac ggcggtgtgt ttcaagacct
    25501 tctcgaaggg aaggccatag gtattgagca gttcattcat tcccgagtgc aggggagcat
    25561 ggtttcttcc gccttcactg ccgctgtggc cgatgaagtg cttcagcgtg gcgagcggct
    25621 gacggttctt cccttggaga ccgttgatgt aggcgatcgc catttcgccg atgagatagg
    25681 ggtcttcgcc gaacgtctct tccaggcgcc cccaacgggc atcccgggag acgtcgagaa
    25741 ccggcgacaa gccttggtga acgcccatcg agcgcagctc gtccccaatc gccattccga
    25801 tctttttcat caactcggta tcccatgccg agccatagtt gagcgaacag ggaaatgccg
    25861 tcgcaccgac actcattact ccgctcagac actcctcatg gatcatcgcg ggaatgccga
    25921 gacgggtgtt ttcaacaagg tggcgttgaa tcttgttgat caccttcacc tgcttttgcg
    25981 gatcattggc gattgttcca tagggacggg tgagctgtcc gacgccgaac tccaaagcgg
    26041 cttccatggg attcggcagg tgattatacg taggaatcgc ttcgcggacg gtgatggtct
    26101 gctcgtcatg aatctcaaac cagaccgata ccaactgggc gactttttcc tcgagatgca
    26161 tttttttcag taatgttgcc gccttttcgc ggtggatgct catatgtaca actccttctt
    26221 caacacattc atacttcata aaaggtttgc tgtgtgtccc tcggaggggt tgttgcggga
    26281 cacacagcag ccaatttatt ccttcaacgc tcccagcgct acaccggcga tgatgtattt
    26341 ggcaagcatg aagtaaataa caaagagggg gagaacggtc agggaaagac caaggtacac
    26401 tgaaccgtac tccgttttat agatgtctcc ccgcagcaag ctgaccatga tcggcatcgt
    26461 gtactttttc ttgttggtga ggagaatcaa cgggataaaa aggttgttcc aggaaccgac
    26521 gaacgagaag atcgcctgag ttgccaaagc gggcttcatg atcgggagaa tgattcgatt
    26581 gaacgtcgca aactcccgcg atccgtcgat ccgagcggag tcaacgatct caagcgggag
    26641 agcggccatc aggtattggc gcatgaagaa caccatcgcc ggactggcga tcgccggaac
    26701 gatcagggga gtgaacttgt tcgtcattcc gatccgatag atgaactggt agaacccgat
    26761 gctggaaagc tgggcgggaa tcatcatgat cgccatgatg aaggagaaga acgcctggcg
    26821 aagcttccag gagtatgcca tcagggcata tgcggtgagc gaggagaagt acaccgccaa
    26881 aaccgttact ccgacgctga ccgtcatcga gttgatgaac ccggtcatcg gactgaacgc
    26941 cttgccggtc agaatcttcc agttgctcat gaaatatttc gatggcacca agccgatcga
    27001 ggactgctgg atctcatacg ttgaacgggt ggcgttcatg aacatgatcc agaacgggaa
    27061 gatcgaaagg atggccagga aggtacagac gaggtagacg atagtcttgt acagcatccg
    27121 ctttgaacgg atcgatccat gcgtgctcat gcgttcaccc cctttcgcgc cgctctgttt
    27181 tgagctctcc gatacttgcg tcgggccgcc tcatccttgt ccttcaggac ttggaagagg
    27241 atgatcgaga gcacaatgat gattccaaag acgatgatgg acgctgcgga ggctcggtta
    27301 tacaggtagc tgccgctgaa cgcctggtcg tggatgaaca tgctggaagt aaccgtggct
    27361 tggtcgggtc cgccgttgag gaagagcttc ggaatatcga acatctgaag cccaccgatc
    27421 atcgaggtga caaggacata gagcaggatg gtcctgagga ggggcaaggt gacacggaag
    27481 aagatctggg tcggggtcgc tccgtcgatc tcggccgctt caaagagaga gggattgatc
    27541 cctaacactc cggcgatcaa cacgatcatc gtgtttccat accacatcca gaattggatg
    27601 aaggatacga tgccccgggc cgtccagcgt tgcaggctga agttcctcgg gccgtcgata
    27661 atatgcaatt tcattaacaa gtcattgacc gggccttttg gataaccgaa caacgagttg
    27721 aacaggatgg cgatcgttgc tgcggtaatg atgttgggca tatagatcaa gattttatag
    27781 gcgccctggc ccttcacctt gaagttgcgg ttggtgaacc aggcggtaag gagcagagcc
    27841 atgctgattt gcggaataaa gttcgctgtc cacaaaatga tggtgttttt caagcttgtt
    27901 gtgaaggttt tattgttgag aaccgttctg aagttcccaa acaggtcgag atttccttcg
    27961 gcggttcgaa gaaaatggaa ttctttcaca cccaatcccc gaaggttggt aaacccgatc
    28021 acgagggtat agatgatagg atagagggaa aacacaagga aggtaacgac aaaaggtatg
    28081 gaaaatatat atccatattt tgcataactc acagattttt tacgcacgat gttcttcctc
    28141 cggataaaag tgcagtggcg aaaaaaatga aattgagaag tggcagagct ctgccacttc
    28201 tctcacgttc aacgattagt tgctgggaat atcaagggtt tcaaacacca tccgcttgaa
    28261 gtcggcaatc gcctgggcgc gggtcttgtt tcctgcggta tactcgcgaa cctgggctct
    28321 ccacgtgctg ttgatcgtct catcatactg ggtgaggttc tttccggttg cgaatgcgcc
    28381 tgcggggacg aatgcttcaa acatatcctg tccgccgagg aagtcaaggg atccgtcgct
    28441 cttgctcatg acggttccgg atgccaccga gtccttcgtg ccgccggctc cgaagagcgt
    28501 tccgttcgcc cagtagtact gcagcccggt ttcggaggag tcgagggtga tccactggat
    28561 cagctcggcg actgcgtcct tgaccttgga gtcgcggttg gcaagaaccc aggtgccgcc
    28621 ccagaagaag ccgatcgggg gctcgcagac cgcccagtca ccataggtgc cttcaccgat
    28681 cttcgttccg ccggagttgc cggccatgac gtagttgatc agccaagcgg gtccgaagaa
    28741 gccgaacgcc tgcttcgggt tcgctccctt catgtcggcg aaccacgcgt cggtccagtc
    28801 ctgagtgtcg ttgtgatagc cattatccat caggcgcttg gagtggtcga tgaacgcttc
    28861 gcgcttcgga tcaagataga gcttgccgtc aacgacccag cccttgtcgg agctgttctc
    28921 aacggcgtgc cagaggtctc cgtctccgga aacaatcgca taacctttct tcttgagggc
    28981 ttcggcggct tcaaagaact tgtcgaggcc ggggccgatc ttggtcttga tcacctgagg
    29041 atcgtcggtt ccccagacgg ccttggcgat tgagcggcga tagatgaacg caccgccggt
    29101 agcctgatag ccaagggcga cgaggccgcg ttcgttgctt ccgatgtcga tcgtgtactg
    29161 ggcgatgtcc gcttgtttta cgaggcggtc aacatcgata ccgagttcct tataggttgc
    29221 tgcatactgc ttcgcatcac cttgggtgta cttgaggacg aatgctgcct cggcggcgta
    29281 gatgtcgggg gcgtccgcgc caccgccgat caaggcctgg tcgagggccg gctgataggc
    29341 gccatcggtg gttgcaatga tcgtggtctt gatttcgtag tcgaactcgg ggtgcaactc
    29401 tttgtagcga tcgatcattt ttggaacttc atccgtgaaa ctccatacgt tgatgacttt
    29461 cttttttgcc gtagtagctt cagtctgacc gccagcgaac aacgagacgc ccaggacagc
    29521 tacaagcagg acgagtgtaa tggttcttac acgttttgcc atggtagagc ctcctctgta
    29581 ttgtgccttg gttgatccaa ggcaccgaat ccattgtatg tcaggcacac atcggtgtgt
    29641 ctgagcaaag gaaccccctt tgctgtctaa actctacctt ggccaaaagc aggatgaaac
    29701 taggcatttg actggataac gcctaggaca attgtcctat aactccctgc ttttcgtcca
    29761 tttgatcgcg tattttctca gctctccggc atcgctcacc gcaatcttgc ggcggatgtt
    29821 gtcacggtag acgttgatcg tcttgaaaga gaggttcagc atctcggcga tttcactgac
    29881 gccgtacccg ttgccgatga ggcggaacac ctctttctcc cgagcgctga gcgtgtcgat
    29941 cggatcgagg ggagtatcgg attgctgggc gaccatcgtg tcgatcatcc gctcgcgcat
    30001 cgcatcggaa agatagatct tgcccttgag aatctcttca atcgctccca tcagcgtttt
    30061 tgcggcctcc tgcttcatca aatatccccg ggctcccgct ttcagggagg tggaggcgta
    30121 gatcacttca tcgtacatcg agatgacgag catcatgatg tgcggcttga gcgcttttac
    30181 attcttcacc agttccagac cgctctcctc accgagggag atgtcgatca gcatcatatc
    30241 cggctcttca gcttcaagga ggtccatcgc ttccgcaatc gatgcggctt gacctatcac
    30301 catatagtga ggattgtctt ccacgacact gaccagtccc tggcggaaga ggggatggtc
    30361 atcaacaatc aaaaacgtag tgggctttct caccggctac tcctctaaac atactgaaac
    30421 tcgggttcct tcggcagatg attggatatc gagtgtcgca ttggccattg aagcccgact
    30481 gcgcatgatt cttaggccca atgcgctttt acgaatgttt ttcgcaaacc cgatgccatt
    30541 gtcctcaacg gatgcttgga ggtgtacccg gccgatttca tcttcacttc gttcaagctt
    30601 gatgacgaca ttttttgact tggagtgttt taccgcattg gtcaacgctt cctggatgat
    30661 cctgaagacg tgcagcgccc ggtcgccgtc ggtaatttca aagtccggat cggcatcgat
    30721 gccgatgtct atcttggcat atcgctgcgt gtcggcaacg agcgcctcga tccgcttggt
    30781 gaaggtcttt tcatctttct cgagggggag caggccccgg ctgatcgtct taagtttgct
    30841 gatcgattcg gtcaacagga gggatatctg ctcaagcgag gagtcgagtt tttcattgtc
    30901 ggcgttgatg ctttttcgag cgctggcggc gatcagggag attccaagca gatgctgggc
    30961 cagatcgtca tggagttcct ggccgattcg ctccatcgtc ttggcgctga tctccatgac
    31021 ttcctgttcc agttctattc gccgtttgat ctcattggag agattcttgt tcgttcggat
    31081 caagtctttt gttcgaaggg ctacctgttc gatcagggtc tgttcatggc tctttatctg
    31141 ctcaagcagc aaggtaccct tcaggctgct ggagatctgc tcctgcagga cgtcgtagag
    31201 ggccgggctc accacgcccg agggaacgat cagatatccc agcggttcgc ttccgtgcac
    31261 caggggcatg aggatccacc ggcctttgtg gaacgatggc ttgtatacgg aaggaaggag
    31321 ctgtttatac tcaaattcaa gaggagagga ttccttttca aagctctccc aggagaggtc
    31381 gatcaaaagt cgggctgaac cctcatccgg agtgaactcg accaagtacc cctcggtgat
    31441 tccgaagagg gcaagacctt gcttgagatt ggtgaagaga gcttccaact caaaggtgcc
    31501 ggcgagcatc gcactgaccg tcctaagcgt ttcaaaggag ctttggagag cgacccgctt
    31561 gctcgcctgg taccggctgc cgcgctcccc cacgaacacc cgggcggagg cgatgacgtc
    31621 ggagatcatc attcgatgct tttcgtcctc cgcctcgatg aggttttcaa tgactgcgag
    31681 gtacacgtgc caacgctcca caacaccgct ttcggcgatc gttgcatcga tcgcccggtt
    31741 cagttgggta ataagccctt tggggttttt cattctcagc aaggcggcga actcgttgat
    31801 cgtccgttct tcaccttggg tcgcatagga gggaatccgg gtgactgtcg agtcgaaacg
    31861 gaatgtcgga gcgcacccgc aggattcacg gtacgtcacc gccgaggaga gaaccacatg
    31921 ctcctcgctg ccgccgtcga tcatccgctc caggatcgcc acggcgttcg ttcccaggtt
    31981 gtgcatcggt tgaatgacgg ttgtcagcgg gggttgggtg tagcgcgatg cttcgatgcc
    32041 gtcgaatccg acgacggaaa catcgtcggg aacgttgatc gagtgctttg ccagaacatt
    32101 caaggctccc tgggccatcc ggtcgttcag gcagatgacc gcatcgaagg gaaggcccgt
    32161 agcaaggatc gtctccatcg cctgttcgcc ggagtccagc gtgaatcttc cgtgaacccg
    32221 caggcgcgga tcgatgccga tacccaattt tgtaagggtg tcgaagaccg tctgttttcg
    32281 attgatcgct tcgtcgtgaa tcgcaggacc gtcgatcatc gcgaactttc ggcggttgtg
    32341 gacggtatag aggtgttgga cgagttccga taccgagggc tcggaatcaa cggtgacatc
    32401 gctcattccg gggatcttca tgccgatcga cacgcggggg atattggccc aagcggaaaa
    32461 atattcacgt aaatccttgg agttcaaaaa tgtcgcgacg gacgaggaga taatgatcaa
    32521 gccatcgatg ttttgatcat tcgcaagctg gtaggcgatg ttggaggaag cctcggatgc
    32581 gatcggagag ccgagaacgg atccaagaaa actgattgag ctgaagcctc gtttgacgat
    32641 cgcatgactg atcgcgctcc acagcgtgga ttgatattca tcatccaggc tcgccgtgaa
    32701 aatgccgatg cgttttttgc tcatcaccat atcagtctat cataccgtat cttttgatga
    32761 atgcaactca tcgaaaggac cgagtttttg aaggtagcgc gagcggtagg cggtggggct
    32821 caatcccgtt gattgtttga actgttttga aaagtgaaat tccgaacaat actggagttt
    32881 tgcagcgacc tctttcaccg agaggttcgt gtttgccaag aaggcacgtg ccgcttccaa
    32941 tcgaaggttc gcatagtact gcatcggagt ggtattcatc caccgtttga agagacggac
    33001 aaaataggaa gcgtcccgct tgacatggga ggcgatgtcg ctgagcgtga gcttgtcaaa
    33061 gatgtggttt tgcatatagt caagggccag ctcgatgatg atgttttcct tctgttgaac
    33121 agggtttgat ttttcagctg ccaaaccgta gagcagtcca agaatctggt gggcggcgct
    33181 gattctcaat tcctgttctt ccttcaatgc tttgtcgcga atctcttcaa agaaaaaacg
    33241 tcgtccggtt ccaagatgga aggggttttt cttttcaagg cgattgaaca actcatggtg
    33301 ttcggcgcac tcaagcaaaa ttgcatagta ggtgaggggg ttttggatgc ttgcttcaat
    33361 ctggtgagtg gtttcggcgg tggtgagaaa cagcgatccc gaagagatcg agtatcgttt
    33421 gttcccgttg atgaaggtcc cgtctccacc gacgaagtaa tggagctcca cttcgttgta
    33481 tccatgggag tgaaaccgtt gatgccaaag caattccgcc gcctcatgca tctgaaatac
    33541 gaatactgca tccttgatat ccatatgtca ataatacata tgaatcgcca atcttgtcta
    33601 tggaattctt ctttcagtgt ggtacgcttc caatggagga aggtatgcat atagtagcgg
    33661 gacttgatgt cggaacgcaa agcactaagc tcatttgtta cgatgcaacc cacaagaagg
    33721 tagtcggaaa agcctcgtcc cctcatcggc tcatcaccgg ggagggggga accagcgagc
    33781 aggaagcgat ttggtatctt gatgcagttc gttcctgttt caaacagatc gatccgtcgg
    33841 taaaggagaa gatcgccgct ctcggagtaa gcggtcagca gcacggcttc gttccggtgg
    33901 gaaagaacgg ggaagcgctt gctccggtga agctgtggaa cgatacttcc accgtagcgg
    33961 aatgtgaaac cctcaccgag cgtctcggcg gcaaagatgc ggttttcaag ctcatcaata
    34021 atgagatcat gcccgcctat acgctggcga agattcttca cttgaagaac catcgaacgg
    34081 aagcatatga acaattggct cacttgcttc ttccccatga ctgggtcaat ttctatctca
    34141 ccggggagta ttttaccgaa gccggagatg cttcaggaac agcctactac agtcccgaga
    34201 aacgggcttg ggagcctgag atgctccggg cggtcgatga gaagaaacac ctcgaacata
    34261 tgcttccgac cctcatcgaa tcgcatgaga tcggaggacg gctctgtaaa accgccgctg
    34321 aagaactggg actgagaccg gggatcgtcg tttccaccgg agccggagac aacatggcca
    34381 gcgcgatcgg aacggggtgc acctcaggcg gcgacctcgt catgagcatg ggaaccagcg
    34441 gaaccctctt cggatacagc gataccctcg tcgccgatcg tcaaggccgg ctctccgcgt
    34501 tctgttcttc aaccggggga tatcttcccc tgctgtgcac caacaactgc acgctttcaa
    34561 cggaagtgat ccgcaatctc ttttcccgcg atgtgaaaac cctcgacatg ttggcaggca
    34621 aggcaccgat cggttcccgg ggggtgacgg ttcttccctt cttcaccggc gagcgggttc
    34681 cctatctgcc gtatggaaag ggagtgatcg ctcatctgga ttcgcagaac accaccgagg
    34741 agaatatcgc ccgagcagcg ctggagagcg ccgtcttcgc gcttcggggc ggaatggaca
    34801 cgttcagcga actcggcttc accccttcgc gcctcatcct caccggagga ggggcaaact
    34861 cgaagatctg gcgccaaatc gcaagcgacg tattcaatct cgaggtcatt gttccctctt
    34921 ccacggaaag cgccgcattg ggtgtggccc ttcaggcgct ttgggcgctc gagggcggtt
    34981 cgatcacgga gatcgccaac gagcatgtgt tgttcgatga agataaaatg catcaacccg
    35041 accggatggc ggtcgaacaa tatgccgagg cgtacaagcg ctacagcacg tacgttcagg
    35101 cccttacacc catttttaca aggagaatat gatgagttat tttgtcggtg aaaaagaata
    35161 tttcaaggga atcggaaaga ttcggttcga aggaaaagca tcaagaaacc cgctggcgtt
    35221 caagcactat aacgccaacg agcgggttgg agataagacg atggcggagc atctgcgctt
    35281 ctccaccgcc tattggcact ccttctgtgc cgacggaagc gatccgttcg gcaagcccac
    35341 gatcgtcttc ccctttgact cgagcgatcc gtttgccaag gcggatgcgg cgttcgagtt
    35401 tttcaccaag ttgggaactc cgttctatgc gttccatgat gtcgacgtgt tcaacgatat
    35461 cgatgatggc cgcgcatatg aaaagcgcta tcaggaggtt gccgacggct tgttggcccg
    35521 ccagaaagcc tccggcgtca agcttctttg gaacacgtcc aacgtgtttt cccacccccg
    35581 ctacatgaac ggggcggcga ccaatcccga cttcaacgtc gtcgcccggg cagcggttca
    35641 aatcaagaag agcttggatg tgaacgtcca gctcggtgga ttgaactacg tcttctgggg
    35701 cggacgggaa gggtatatga gcctgctgaa caccaatatg aagcgggagc aggaacacct
    35761 tgcccgcatg ctgatcatcg cccgcgatta cgcccggtcg atcggcttca ccggaaagtt
    35821 cctgatcgag cccaagccga tggagccgac caagcatcag tatgactatg atgccgcaac
    35881 gacgatcggc ttcctccgac gttttggatt acaggacgat ttttcctgca atatcgaagc
    35941 gaaccacgcc acattggccg gccacacgtt cgcccacgat ctgcttgtct cttccaacgc
    36001 gggaatgctc ggcagcgtcg atgcgaacca gggtgatccg cagaatggct gggacacgga
    36061 cgagttcccc acgaacctct atgagatcac gatggcgatg ctgatcatcc tgagccaggg
    36121 gggcttgggc ttgggcggat tgaacttcga tgcgaagatc cgacgaaact ccaccgatgc
    36181 cgaagacctc ttccttgccc acatcggcgg aatggacgcc ttcgcattgg ggttgaagat
    36241 cgcccacaag atcaaagaag agggcttctt cagagacttc gtcgacaagc gatacgcctc
    36301 cttcgacagt ggagatggaa agcgctttgc cgaagggaag atggatctcg ttgaactggc
    36361 caggatcggc ctggatgcga agatcacgca gaagagcgga aaacaggagc tgctcaacaa
    36421 catgttcaac tcctatctct ttgattatta agttgtttgt accatcaaag gaagccgcct
    36481 cggacagggt tccgttcggg gcggcattct ttatgcgccc agcaggaagg tgggctgctc
    36541 ttcggtcatg tacaccaggg tgttgatttt cgccgcgttg cggcgaaaat ttgaaagcgc
    36601 ccggaaagaa aggagaagca ttacatggca agctttgata tgatttcaaa agatttgagc
    36661 tcacatgttc aatatagaaa aagtgaaaat agtgttcagg atttaaagga aattatcgat
    36721 tctgcacaga gctacgcttt tagttcggca aatattgctt tggtattccg gaattggcta
    36781 atcgggaaac gaatatcaga agaagaattg caaggagaga tgcgtgccga gtatggtaca
    36841 taattgctaa aaagccttgc taaggaaatg gaaaaatctt acggaaaagg acttaatcaa
    36901 agaaatcttt actattatgt tcgattttac gattatttcc ctcaaatttt gaacgcagtg
    36961 agttcaaaat ctccgatact t
//
LOCUS       DAOSLI010000014        35217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1399_length_35217_cov_24.025482, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000014 DAOSLI010000000
VERSION     DAOSLI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 35217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35217
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1399_length_35217_cov_24.025482"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PLF67_03490"
     CDS             complement(<1..1222)
                     /locus_tag="PLF67_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOE89112.1"
                     /translation="MSVELLSPAGNLEKLRLALEYGADAAYLGFSPFSLRTNAGNFTD
                     ADLGEVSALKERTGKKLYCTVNMLFDQNRLALLEDQLPKMKAWPFDGFIISDMGIVSL
                     LRKHLGADVELHLSTQASCTNSESARFYRQAGFKRIILGREATLDDIKAIKDKNPDLE
                     LEVFVHGAMCMAYSGRCLLSAHLAGRSGNHGDCSHSCRWNYRIALEEKEREGIYYPIE
                     EYEDYTTILSSKDLCMIDHLDELIEAGVSSLKIEGRMKSSYYVAVVTRAYRKALDKDP
                     HWEDYREDLIDVSHREYSTGFFFGSGPIDPTKGSMITKKDYQQRTLFCGFIREQKADN
                     LYRLELKNSIRTEHILEIIAPDLPNFALDDFILFDEEMEKIPQGDHNKPCYLRTDVAL
                     RDGYIIRRSLEVKPE"
     gene            complement(1219..3687)
                     /gene="lon"
                     /locus_tag="PLF67_03495"
     CDS             complement(1219..3687)
                     /gene="lon"
                     /locus_tag="PLF67_03495"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOE89113.1"
                     /translation="MSEQELMPIEQLLPNNLFILPVSGNPVFPGLFTPLMITDKSDVE
                     IVNQAVKHGGFLGLLLVKEDVETETVGAEDLYTVGTVAKIVKKIKLPDGGMSIFISTL
                     KRFEIKQFYPGGKYIIAEVTYLEDIEDNREELRAWTRLLLNEMKSLTKNNQLISEEMR
                     LNMVNIDHPGKLADFIASILNVDRTDQQDILETLIVRSRIEKVLVFIKNEQNIAQVQA
                     KIQARVNKKLEKNQRDYFLREELKSIQQELGLTTNPKAELIERLTKKFKSLPLNEEAR
                     ETVERELSRLEMMESTSPEYSLTRTYLEIISDLPWKDPKPETFSIDSARRILEKDHYG
                     MKDVKDRILEFLAVRKKKQDTKGSIICLVGPPGVGKTSVGVSIARALKKEYFRFSVGG
                     MNDESEIKGHRRTYIGAMPGKIVQGLRITKSKNPVFLIDEIDKMGISYQGDPASALLE
                     VLDPEQNSAFRDTYLDIPFDVSQVLFIVTANTLETIPRPLLDRMEVIRLSGYTSDEKL
                     AIGRKYLVPKSLEKHGLNKQEIKYTSPILRKIADEYAREAGVRNFEKSLDKINRKVAL
                     KLVEQPDLDTPVVLRQEDLVEYLGQPVFVEDEILKADKIGMALGLAWTSMGGDTLIIE
                     AQNTPGKGEVKLTGQLGDVMQESVSIAHTYIKAHAEQFRIDPLWFDHNNVHLHVPEGA
                     TPKDGPSAGITMTVALYSLITNQVMAPNLAMTGELTLKGKVMPIGGLKEKILAAKRNK
                     IKEIIIPQFNKRDLDELEDEVKKGVVFHSVATIEEVLRLAFPEDAKRKLKPFHPSQET
                     SQSQAKEVVSIAEAVARAVKEALS"
     gene            complement(3772..4398)
                     /locus_tag="PLF67_03500"
     CDS             complement(3772..4398)
                     /locus_tag="PLF67_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOE89114.1"
                     /translation="METWTKKDLFLFSLYITGMVLVNTVGSKIISLFGVRVSVGIFFM
                     PVLFLITDIVGEVRGRKQVTHFVRFSTIMLALMLVVTAIFVSVKPHPTWDLQQPYQQV
                     FGMSMRMSLASLISFGIGQYVDVVIFMTLKELTKSKMLWLRNNVGTITSQFIDTVIFM
                     FIAFYKLTPTFTVSYLFSLIIPYWLFKVVFALIDTPFCYLGVKWLSKE"
     gene            4507..5454
                     /gene="arcC"
                     /locus_tag="PLF67_03505"
     CDS             4507..5454
                     /gene="arcC"
                     /locus_tag="PLF67_03505"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOE89115.1"
                     /translation="MESKLIVIAIGGNSLIEDPKHVTVQSQYEAARKTAGHIARLVQA
                     GHRVLIAHGNGPQVGYVLLRSDIAKDVLHQVPLDSCVADTQGAIGYQLQMALHNELAE
                     LGVTRPVVTVVTQVEVDANDPSFYNPTKPIGSFLTRNEAEERKTKDGWNVVEDAGRGY
                     RRVVPSPKPKRIVELDSIRSLLENGSIVIAAGGGGIPVIRDEDGMLRGKEAVIDKDLA
                     AALLAKELNADLFVISTAVEKVFLNWGKPTQKSLDLITSTEAKQYIKEGHFAPGSMLP
                     KVEAIVDFVEVTNNTGLITDPANIHGALYRDKGTKIVKG"
     gene            5441..6001
                     /locus_tag="PLF67_03510"
     CDS             5441..6001
                     /locus_tag="PLF67_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89116.1"
                     /translation="MSKAKRFFPDLKSLIVSTVVILLLLIFAVVVTDHNNVRRLHRYL
                     WEAEAAKEACYSLIEQRLGYAKALVRIIDNQVDTGRVEEVIGQWDGAASVDEASVLYK
                     TLDDELALLQRKAVEHESYRAWSPYFDRMYLIEMELTKASAHYQERAEFFNAQKGGFP
                     ARLAAKRLDLEDLLLFDFGSSLKGRP"
     gene            complement(5960..7198)
                     /locus_tag="PLF67_03515"
     CDS             complement(5960..7198)
                     /locus_tag="PLF67_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOE89117.1"
                     /translation="MKRLIGVTTLLLLSFALFSLSVVPLEIEQPYDSIESLYYANFGP
                     SLAQNQEDWVAKTRIQLLTVGPGEDLYAWFGHTALIVTQTNGRKIMYDWGIFDDTKEG
                     FYRDFAFGKMYYRVSVSDALWRIEDSIAEGRDVRIVDLNLDPSAQFALIDFLYHEIGP
                     TGRTYLYHFYDDNCATRVRDILDKATGGQFKAWAKSREGTSLRSTALPYMAHDPLLGL
                     GLNLLQSWRIDKPTTLWDEMYLPETLHQAVLDFTLADGQHFGINNTVLNEGSLSPPKH
                     DFLTIDALFFVGLITSMVLIILGLYLPRFTSLLYGILFIALALVGTLLFVLMVGTNMN
                     VTYWNLNILFINPLLFCVAFRPKKQGKVLLFGLGSMIVLLALELLNAPFHQDSTLLIA
                     LLLPIYLSRAAFQGRTEIKQ"
     gene            7220..7999
                     /gene="recO"
                     /locus_tag="PLF67_03520"
     CDS             7220..7999
                     /gene="recO"
                     /locus_tag="PLF67_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOE89118.1"
                     /translation="MPIFQLVDFFFPALYPDDMERNVSSRAIVVHSQKQSQMNRRLTL
                     LSVDFGLIEAISYGSAKSMRAPKANVFANATVYLYYNPVRDHYTLKDVAIIESNEHLR
                     SEITLTYRGLFMAELIMKTHGGESELEYELLSQGLRFLQSGIADRVLIQFVLRLARIL
                     GVHADYEACPVCSRKYRDDETLRFTTDLLSPCCSECGSADLSLPPGARRYVKLTSTLE
                     YGESLNVPLSETATARIKGYALSYAKLLAQGPLKTLQSGLL"
     gene            8020..10176
                     /gene="recJ"
                     /locus_tag="PLF67_03525"
     CDS             8020..10176
                     /gene="recJ"
                     /locus_tag="PLF67_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOE89119.1"
                     /translation="MDDRVWVKEPVESEAIKRLLEEYQFSTLQATILSRRGITEADDV
                     KYFLEQELSYLHNPFLFDEMEDVVDRILDAVQEEEKVRIFGDRDVDGITATALLVEEL
                     RRLNVDVSYSLPTGDEPYGLLQSGVEQAASDGVTLLITVDCGIGNIEEIVLANSLGID
                     VIVLDHHIEGEELPPALAIINPKMSGSEYPFVHLAGVGVAAKTIWALRFARTDLYRQE
                     LILLHAQPGNDTVIIQAMKVNNLLVTDRIIEEINPGVLKTERSKALAFLSCNVPILVL
                     DAAVEHAQLSKAFGKSVDIHLLDLRNELEKVLPAVRNRGLFALTQLSRAARYVKGGRD
                     ELTTLYSLFIAYVLKKHPALDDEYERLLDLVAIGTVADLMELKNENLLMVRRGLKVLS
                     TAPRPSLVPFLASLGLLNRRISSTEISWRIAPPINSTGRLGTPAVALEMLLSTDPNEI
                     ERLTSEVLAMNTERKRLSDEIWNVVRGKAQESFERTGSKMVILQDEQIEMGVASSLAS
                     RLVKAYNVPALVLSSSRSGRIKGSMRANDSTIHTRNFLEHFDRQGSYFLYYGGHANAS
                     GFTMESDRLEELMKALHEYVDTIDCPEERVKEYFIDATISEEEMHPELIRLVEFFEPY
                     GEGNRPLVFEIQGAVVEEIGYLNNSQGGDRHVKLTIGYGHYRWPAVYWSAAPLVGKVF
                     DRGSVLDILFRLARNYWMGNETLQLNIVDVKPTKAL"
     gene            complement(10163..11356)
                     /locus_tag="PLF67_03530"
     CDS             complement(10163..11356)
                     /locus_tag="PLF67_03530"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOE89120.1"
                     /translation="MNSRLERELTEFLTSLKEEGLYKEERIIEGSQGREILVGGKTVL
                     NFCANNYLGLSDDKRVIRAAADAMETFGFGLSSVRFICGTQTLHKQLEQAISSFLRTE
                     DTILFSSCYDANAALFEPLLGEEDAVISDELNHASIIDGIRLSKAGRFRYKHSDMNAL
                     EQALKASASARRRLIITDGVFSMDGDVAKLKEICALAETYDALVAVDDSHATGYLGET
                     GRGSVELCGVEGKVDIITTTFGKACGGASGGCISGRKPLIDLYRQRARPYLFSNTLAP
                     AICGGTLKVLSILQEDHSLRKRTLANADHFRNLLKTAGFDLIDATTAIVAVMVYEEAK
                     AVEFAEKLLNEGIYVIGFSYPVVPKGKARIRVQLSASHTTEDIERAAKAFISVGKTIG
                     IITEL"
     gene            complement(11353..12306)
                     /locus_tag="PLF67_03535"
     CDS             complement(11353..12306)
                     /locus_tag="PLF67_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016522844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE89121.1"
                     /translation="MKRILIIGALGQLGSEIALECRRRYGTDNVVLADIRSDVNRPLI
                     EDGPFYLLDARDGKRIDEVVRTHRIDTIYHLAALLSARAEQDPMEAWNLNINGLIAVL
                     EVARQRSCAVFVPSSIGAFGPTTPKKFTPQDTIQRPTSIYGVTKVTGELLCDYYHHRW
                     DVDTRGIRFPGIISSLTPPGGGTTDYAVEIYYEAIRNQHYTCFLRGDTYLDMIYMPDA
                     VEAAIKIMEADPAKLRHRNAFNIAAMSFCPEEQAAYIRTHIPTFTISYAIDPVRQAIA
                     DSWPDYMEDYAARVEWDFKPRYTLASMTEDMIGKIREREGL"
     gene            12421..12993
                     /locus_tag="PLF67_03540"
     CDS             12421..12993
                     /locus_tag="PLF67_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOE89122.1"
                     /translation="MQTPPMIGKNIQRIRTARKLTLNVLSERSGVSKAMLSQIESDKV
                     NPTVATVWKIAKGLNVELNDLLEGVDEPTRVFSLNPASDDGSKLEKRKDGITIKILSP
                     LSSVEDLEMYLLTFEPNSTLASEPHVAGSQEYLTVVKGSVDVRAGENSAHLKPGDFLM
                     YHADIEHSISNQSGKEAMVHMVVRFSSDIR"
     gene            13085..13294
                     /gene="rpsU"
                     /locus_tag="PLF67_03545"
     CDS             13085..13294
                     /gene="rpsU"
                     /locus_tag="PLF67_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOE89123.1"
                     /translation="MAYVRVDENEPLEKSIKRFKRMVEKEGIIREYKRREYFEKPSTL
                     LNRKKKALARKQMKKARKQYSSKGY"
     gene            13504..14388
                     /locus_tag="PLF67_03550"
     CDS             13504..14388
                     /locus_tag="PLF67_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE89124.1"
                     /translation="MLILVADAFDASLPGRLSVFGEVTTDHNRLSEANVVLVRSKTKC
                     TKEYIDGAPNLKVIIRGGVGVDNIDTEYALSKGIVVRNTPTSSSIAVAELTMALMISV
                     PCNLIEYHQGMKEGNWLKNLKRTELYGKTLCLLGMGNIACKVAERAQAFGMNVVAYDK
                     YVTTHQFAKMLPSAEIAVENADYISLHLPLTNETTNLVNEELLSHCKKKPVVINTGRA
                     LCVDADYMAKALKGGSVSWYCTDVYPTDPPAGDYPLLDCDRVTFTPHVGANSVENLGR
                     IGQETFNIIKELLEGGII"
     gene            14388..15470
                     /gene="serC"
                     /locus_tag="PLF67_03555"
     CDS             14388..15470
                     /gene="serC"
                     /locus_tag="PLF67_03555"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOE89125.1"
                     /translation="MERKKNFFAGPSVLPLEVLKQVQEELVDYKGNGLSMIETSHRGG
                     MFEEMYDETLALFKRLIGAGDEFDFYFLGGGATLQFSMVPINFLPQGGKAGYINSGSW
                     SEKALIDAKKLGNIDVVWDGKESGYTTLPKMETLRVDPSLSYLYLCSNETIGGIEYKE
                     FPKTDSVPLIADMSSDIFSRPFDVNQFGMIFGGVQKNLGPAGAAFVLYKKELLEKQNP
                     NLPSYLDYALHGKNKGLANTPPVFAIWVVNLVLKWIESHGGIEGMAKRAETKSSLIYD
                     VIDSSSFYRSPVDPSYRSTMNVVFRLPGEELEELFIKESKKEGMLGLKGHRSVGGLRA
                     SLYNSLEPAAVEALAQFMREFETRHG"
     gene            15463..15930
                     /locus_tag="PLF67_03560"
     CDS             15463..15930
                     /locus_tag="PLF67_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOE89126.1"
                     /translation="MDKLMKIKMDETNKAILRQLIDGRKPFSAIADELNITENTVRSR
                     VNKLIDEEVLQISGLIRSEMIPGLQVVFMGVKLSTLDLERKAKEFCTLRGVISSVVVT
                     GRYDLIVQLVLSEEEDLSLLDFFKEELSKMSEVLEVETFVVYQSANYRIPYVL"
     assembly_gap    16061..16160
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16176..17168
                     /locus_tag="PLF67_03565"
     CDS             16176..17168
                     /locus_tag="PLF67_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOE89127.1"
                     /translation="MADLSSTYLGLPIRNPFVAASGPLTASLDSLKRLEDAGLGAVVL
                     KSIFEEQIELDGMTALKDAESYLGHTDGYEFVKATSMEQQIDAYLGLLEGAKKSLAIP
                     VIASINCYRDGTWLEYAKRFIDLGADAIELNYYVVAADPETEGREMEKEFLQLVRSAR
                     KSINVPLSLKIGCAYSSLANLLRRFDEEEVDGVVLFNRFYSPDIDIEHMKITQAQMIS
                     SKDEYALTLRWVGLMSEEVRYDLIGATGIHSADTAVKLLLAGAKAVQLCSVLMKEGVD
                     SVAKMLGDLEVWMKKHGFKDIEAFRGKLAKKYKADGSEWIRVQYLKTMLEAQRM"
     gene            17172..18305
                     /locus_tag="PLF67_03570"
     CDS             17172..18305
                     /locus_tag="PLF67_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOE89128.1"
                     /translation="MDINSYRDIAPYRGQDVLDAVERVKAHEKAIAQFIAMLDPPRTN
                     DERLALQESVKHIVSLLDHVTTYEEFQRTITAGFFLPKIVEKSVTAFTHSGAEKLAND
                     QAYLYVSNHRDIILDCALIDLALAQADQMLMEMAIGDNLLTNQFVTDLFKLNGGIVVK
                     RTLPLREKYLESLRLSAYFVESISERNQSIWVAQKSGRSKDGIDETNPAIIKMLHLSQ
                     KRKGVSFSEVIKLSRIVPVAVSYEYDPLDVNKAAEQLRKQSDKRPYEDVISMNKGLKG
                     FKGRIHIAFGTPLTEAEYHTPEEVANEIDRQIHLNYKLWPTNYFAYDHVNNTTTFAEQ
                     YRDFNHETFLRRFAFRREEVRDFALNAYANPVRSFLSQQARLS"
     gene            18448..18876
                     /locus_tag="PLF67_03575"
     CDS             18448..18876
                     /locus_tag="PLF67_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89129.1"
                     /translation="MSFEVTSDDSVLEVQVSTPDKTSRWVVSVKRDKQGRFTVGPLSM
                     GKGNTLTEGMYSMVILNEQGKTIDYSFPVRSPNTTVDPLQWGSFDSDARTLVMNRSAT
                     LQVGNDHIDLAEGESYIFAPDDHEFYLAFDQGATIVRVTL"
     gene            complement(18873..20624)
                     /gene="mutL"
                     /locus_tag="PLF67_03580"
     CDS             complement(18873..20624)
                     /gene="mutL"
                     /locus_tag="PLF67_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOE89130.1"
                     /translation="MSSRMPIARLNPLVAQRIAAGEVIERPSSVIRELIDNSLDAGST
                     SITVSVTEGGIEEMRVLDDGSGIAKGDLPLLGESHATSKIRDLDDLYHITSMGFRGEA
                     LYSIAAVSSITIASNGYEITIDNGKRGEILSSTTASGTLVIVKDLFGELPARRQFLKR
                     AATEAAMVRTVLQEKALAYPNVAFKLIVDGSLRLNLPVTTAKDRVVDVLTISQRVVKE
                     ELTELFDDHGRFSLYAVASSAALWRSDRSHIKIFVNKRPVDEYALVQAVTYGYSNLLP
                     GGSFPYCYLFITIDPSLVDFNIHPTKREVRLRNKAEIHHRVVRMISSQVRRPIPRLQE
                     KKESPSPTLFEEKNDHLRTHSTVGERSFAYTPKEQIDPSWFAKAREALKEPAFETKRT
                     DEEADIWTFQQSDESFTYLGQAFNLFLIAQKGDDLFLVDQHAAHERILYDQVRAKQPI
                     QNLMIPIVFEVERSIDEYLMSNLDSYLNLGIKLRKVEDLAWELLGVPALYRTIEHEVI
                     AFLQTMQGEPSEIEKGLYSVVACHAAIKAGDRIDSTAATELLKSVFALEEPICPHGRT
                     FVIRLNKDDLMTSVGRT"
     gene            complement(20614..22647)
                     /locus_tag="PLF67_03585"
     CDS             complement(20614..22647)
                     /locus_tag="PLF67_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89131.1"
                     /translation="MKRLETFILVILLTLIAPLSIFGQEYVSVIEGDLASLRLYEPNG
                     KPISIETIDINDLQLGTILVSGDEPIILETSRGDIRLFPDSILILDEVSATSTTLLLI
                     DGSVTAKTTQGTLSIITPATLYELDRAGEVFVTSTRNEERVQSFDVAVRATNLITHKT
                     TTIEALQELELSNPKLAKRTLAPVEAAAGRIVRPARIPAKPGAIQWTVGLEFVEEAEE
                     IKELVRSLPEKPVVLSPSVKAMPVVKEAVAKPRTTLRDSAPAPIQSKGTYGIEAGYTL
                     TFPIKSASYWPGHRLEVKPFISYNSLALRLNVHVETDSFTAFDTNFLNLDPSALGIVS
                     FVFGIVDHVKVGYSTSPFYLLIEEGGYPGTQLAPFIASTFPSGKMALYNSITIGGFKL
                     TTSFDDLRFTNIINDSGSQLGSTVLEYTFGGSYPMGIALGTLAILDSQSSRIFKAELY
                     PVLELSFPIINTRLTQFSALLLASGYLPVYPSVKFEEFFDMNSTYFFPNHQLGVGLTV
                     NHKPFNGQIMASLAKGKNHLLLFNDIAEAHDHIDHNGFIDVYASGTWKSKNLTANAIL
                     NVPFTKEFALATVKGETQDADFSQIGLTYTADVFHLSLGASRVGFLRTLKTAFEGGSL
                     KPLFYGKWASSFLEASYTYKIFTFTTRLNVPIILNNQKPSVDISVKVRFDGVF"
     gene            complement(22669..24024)
                     /locus_tag="PLF67_03590"
     CDS             complement(22669..24024)
                     /locus_tag="PLF67_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89132.1"
                     /translation="MLLLFPFSAGAEEVAQPPQKEGDFRVHNHSTSITILNPLDGLGE
                     ESTDDGLIQAVALSDGSFGVIIKPALSYGPFSTRLSFPLKGRFTFDPFDFQFDFSDWI
                     PPERGAEESALEYALQLLHHYSRFIQALQFGNPYDPIYIRYGKLLGPTLGDGALINGY
                     VDRSVGLLTSKPGLNLMIDASSLTGVQSGFHFLVDDLFDPAMKAWRIYSNPFYEKAFL
                     SSLQLGFSYAHGMRVIDERTIERHFFSLDFAHPLVEKKNIKSDLFIDVLFQGFDISIP
                     QEAIGIRGGINGRLGRLFTYNFALTAPVYGNYYASFFSNEWAIEHEQQMILPPGSSRF
                     ESLISFTWPKQEIFFGAKIASDLTDGVFENTRLFANLRFDRFLFNILSLDLRYEKLYP
                     NERDGSFFDELMALRMVDIQALAIIKVKPYVISLGVSLAYDENTHLTSTIDAVVRIVL
                     L"
     assembly_gap    24400..24499
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24717..25466)
                     /locus_tag="PLF67_03595"
     CDS             complement(24717..25466)
                     /locus_tag="PLF67_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE89133.1"
                     /translation="MSEKLPYQTLLEGDLPPWVMRLVKATGRAINRYSMIRDGDTILL
                     GTSGGKDSLALALALSLRKKWLPIDYTLQAVMINWKEHPIPEEFREKLAVFFTELGYA
                     FHIVDEEQRPPSFKGEFNCYLCSRNRRRILFDWAENEGIVKIALGHHLDDLVETSLIN
                     LCFRGSFDTMKPVQPFFNGKIQVIRPMIEIHERQIQRLSEIYDLPVIKPVCPYDRTNI
                     RSQLKPIVRSLVKIDPLTREHIYKAHHFLDD"
     gene            complement(25459..26715)
                     /locus_tag="PLF67_03600"
     CDS             complement(25459..26715)
                     /locus_tag="PLF67_03600"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE89134.1"
                     /translation="MSLTYTRFLTDSSFTHAQTFRDPLWKDISIPTYMAEVVKSPSFI
                     KLGRIKQLGVAFHLYPGAVHTRLNHSLGVFHLSRQIIGSLLKRKADEGFLTIDGVRHF
                     LAATLLHDIGHFPFTHSLKELPLKSHEQLATELLENDRILRTALKDAALDTKIIGDII
                     DEERPTNNEEALLYRSILSGALDPDKLDYLNRDAYFAGVPYGIQDVSYITDHLYLHEG
                     ELAIGFEALGSIEHLLFSKYLMYRNVYWHTSVRSATAMIKRCLYENLKRGTLDEDGLY
                     GLDDESFFLLSQTERPLGNLLTRVRENRLFQPVVEELFDEKNPIHTNALSLRQRSEYE
                     ERIEKDLNLIGGSIVIDIPERVSFESDIPVVNHQTFLGRFEDVDPLFTRSRAMEFTSS
                     LRTVRLFADPSIDPTVLRHAWEHIHE"
     gene            26781..27995
                     /locus_tag="PLF67_03605"
     CDS             26781..27995
                     /locus_tag="PLF67_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOE89135.1"
                     /translation="MNTYLIIADDFTGSNDSALELAHRGLSTIIGFSSPKGTVDSYVL
                     DTESRNREGDEAYRMTKALIKDLDIDSYDIVLKKIDSTLRGNILEEIRAIDEKMNSDL
                     ILVAPAFSQMGRTCVGGTVFVNSVKLLDTEFAHDPLKPVTEDNLITLFASEYSTIHLS
                     VDEIRSKAFPLIKDAQVIVCDAEHEADLFETVKWARGLDKRILYVGSAGLAGALIQYD
                     RPKQPALAILASLSKASRAQLDYAKSRGVAILAVDVLSILKGGDFTPIIDQGIQILQG
                     GEDLILTLSSALDRSVFQQSIEVGKALGLKDAVVGTMIKDRLSKVSLEIIKASGVKNL
                     FLSGGETAFGLMQMMGINEVKIIGEVDIGIPITLVLDGPYQGMKIVTKAGGFGKEDAL
                     TYSLRRLEERQL"
     gene            27992..28957
                     /gene="pdxA"
                     /locus_tag="PLF67_03610"
     CDS             27992..28957
                     /gene="pdxA"
                     /locus_tag="PLF67_03610"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOE89136.1"
                     /translation="MKIFGITLGDPCGIGIEVTIKALLKEPSYQKEALLFGPLSVLEY
                     YNKRLKANFTFNVIETIEEFKMGMINVVDPHPAKLEDVLIGKVSALGGEVAFEAVRTA
                     IEFARRKEISSVITAPLNKEALHLAGHRFAGHTEIFAHYAKGDSYAMLLSSPKLTVIH
                     VSTHQSLRSACDAVTKKRVLEVIRLGAETMKKFGYPHPRIAVAGLNPHAGENGLFGDE
                     EIRQIIPAIEAAKKEGHDVTGPIAPDTVFLRAIKGDWDIVVVMYHDQGHIPLKLLAFD
                     DGVNITVGLDVVRTSVDHGTAFDIADKLIASEQSMLKAIELGKKL"
     gene            complement(29024..30373)
                     /gene="larA"
                     /locus_tag="PLF67_03615"
     CDS             complement(29024..30373)
                     /gene="larA"
                     /locus_tag="PLF67_03615"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOE89137.1"
                     /translation="MKVINPIDPMKKELILPDTTAVYAMKAPPALANADKAIMDALNN
                     PIESESLATIAKAKLAVNKDATAVIVISDNTRPVPYKGEGNILTPIIQVLFDAGFKAD
                     RITALVSTGTHRPMTDEELEAVIDPWVYEQGVRVVNHDCLDDDGLTYLGKTERGSEVW
                     ISSLYLQSDLKILTGLVESHFMAGVSGGRKSICPGLISEMGTFLFHGAPMMGHPDSRD
                     LLLEGNPVHEESLAFAKMAGSDFIVNVTLDHAFKITGIYAGDMEAAHLAAFEMIRGYA
                     KIPIESEADIVVTHAGFVGINHYQSAKAAFAAIGAMKENGYLISIANYTDKKDIVGSV
                     NYKTVLAILANHGSKALVKLLHSPDWPFTPDQWQVQKWAAAFDKIPLDHYYHYAPQIE
                     AGREAGLPGINAAKLTDKRDYTSVVKAAIEDIYRREGTRDLTILYLSDGPYAIPYVK"
     gene            complement(30451..31479)
                     /locus_tag="PLF67_03620"
     CDS             complement(30451..31479)
                     /locus_tag="PLF67_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HOE89138.1"
                     /translation="MKMGKVPILDTINKIPGGLMVVPLVLGVLTNTFFPGVLNIGSFT
                     TAFFKNGALTLIALLFLCSGAQINLKTAGVAVYKGVVLNTSKVLFGVFLGVIFAKVAG
                     PQAAFLGVTPLAMISAMSNSNGGLYTALASKYGDESDVGAIAIISSNDGPFFEMMFMG
                     IAGVANIPIVALIAVIVPIIVGMILGNLDEKFREFLKPGMFIAIFTFSFPLGAGLSLQ
                     TLGQAGLPGILLGLLTLLITGVPSYFVYRLLIPKRMRVTSAVGAGVGTTAGNALATPA
                     AIALVDPAWAPYVDKATIQVAASIVVTAILVPFLVDFFYKYELKRGLVTRPIADIGGK
                     IEDLGEQL"
     gene            31503..32036
                     /locus_tag="PLF67_03625"
     CDS             31503..32036
                     /locus_tag="PLF67_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89139.1"
                     /translation="MYIYTLSHSLRMSRNIEIGNPRNVTNTKDDCLLDGGWMKRVLLF
                     LILAVSAPGIGAASLEEAFSYKLPVDFYIGQIIEPAENSIEEQSLEALKEPYTPAWVE
                     TYIPEGMRQGFVHTYDHLLSSYLPSEELQIGKPVKIGALVEIPFRMFSPKPLIGLLVW
                     VENDEGDPFLLSLSISE"
     gene            32197..32721
                     /locus_tag="PLF67_03630"
     CDS             32197..32721
                     /locus_tag="PLF67_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89140.1"
                     /translation="MRTQLEHFHKRARLYVLGAAILIILFALFIPNVTAKVILIILTI
                     IAFAVLAIVEWLKKDDVTSKKDDARQAAKASVEKQKPEETKRKAEEAKKAEEAKKAEE
                     DKKTEAAKKTKAKKTAAPDDGVTTRTGRYCVEDGTYYCSEHPDRTVEMVVGKRFPPCR
                     GDGKGHSAIWTLSK"
     gene            complement(32771..34720)
                     /locus_tag="PLF67_03635"
     CDS             complement(32771..34720)
                     /locus_tag="PLF67_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HOE89141.1"
                     /translation="MDKISLNNLNQEQELKQQEILEQFEKESTTRKFDSYALVIGIYW
                     ISIAIALYHFITAAVGYPATHLHRSLHVAMMLFMTFFLYPIGKKSSRKTIPWYDIALA
                     LASIAVAVYVWVDYVGFINRMGSPNLIDVIFGTMLIILVLEASRRISGWPLVILSLIF
                     ILYGLVGRSLPGIFMHRGYDWRAIVNHAFINTEGIYGTSVDVAASYIFLFILFGTIMN
                     KCGMGRFFNDLALAFAGSTKGGPAKVAVIASGLLGSINGSAVANVVTTGTFTIPLMKK
                     SGYSKEFAGAVESSASVGGQLLPPIMGAAAFIMAEMLGVKYGTIVISAAIPALLYYLG
                     IMVQVQLRANRRDLKGLPKEQLPKVRDVLKDRGHLLIPIVVLLYLLLFSGLTVIYSAF
                     WSIVATIVISQTRKSTRMTIRQILDAFAEGTTATVSVAIACAVVGIIIAIVSLTGFGL
                     NMANAIIQLGRANLMLTLVLTMMTCMVLGMGLPSIPAYLITASMAAPALIKLGIPALA
                     AHLFVFYFAMFANITPPVALASFAAAGISGGDPMKTGLQSVRLSLAGFIVPYIFVYNT
                     ALLLLDTTPLVALRVTITAIIGVSMIGMATEGFALTGMKWIERILAFVGALLLITADP
                     IQDAVGFVILVVILFFQFRKKRRTA"
     gene            complement(34693..35187)
                     /locus_tag="PLF67_03640"
     CDS             complement(34693..35187)
                     /locus_tag="PLF67_03640"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HOE89142.1"
                     /translation="MALLLLVPVLLLLGGAACRIRMSEDPVIRVRDQEHGTVLASMPV
                     KAGDDLVFEWIHSYEHIPWIEEYTVTEDLAFRLDAIRVAGFGAGIPNDKGETTVENGM
                     VVMRNINETFPSFAWIHSETALVSITVGGKTLIEGGDLPHHKAVTLTLEGIQRLWTKS
                     PSTI"
BASE COUNT         8557 a         8803 c         9483 g         8174 t
ORIGIN      
        1 tttccggctt cacctccagc gatcgacgaa taatgtaccc gtcgcgcaat gcaacatcgg
       61 tcctaaggta gcacggtttg ttgtgatccc cttggggaat cttctccatc tcttcgtcga
      121 acaggatgaa atcatccaag gcgaagttgg gaagatcggg ggcgatgatc tcaaggatgt
      181 gctccgttcg gattgagttc ttcagctcca agcggtagag gttgtccgct ttttgttcac
      241 gaatgaatcc acagaacaga gtgcgttgtt gatagtcttt tttggtaatc atcgatcctt
      301 ttgtcggatc gatcggaccg gatccgaaga aaaaaccggt tgagtattcg cggtgcgaaa
      361 catcgatgag atcttcccgg taatcttccc agtggggatc cttgtccaga gctttccggt
      421 atgcccgggt taccacggcg acgtagtagg aactcttcat ccgcccttcg atcttcaggg
      481 aggagacccc cgcttcgatg agctcatcga gatgatcgat catgcacagg tccttggagg
      541 agagaatcgt cgtgtaatcc tcatattctt cgatcggata gtatattcct tcccgttctt
      601 tttcctccag tgcaatgcgg tagttccaac ggcaggaatg ggagcaatcg ccgtgattgc
      661 cgcttcgacc ggccagatgg gcgctgagca ggcaacggcc gctgtacgcc atgcacatcg
      721 ccccatgaac aaagacctcc agctccaagt cggggttttt atccttgatc gccttgatgt
      781 catcgagggt cgcttcccgc ccgagaatga tccgcttgaa tcccgcttga cggtagaacc
      841 tggcgctttc gctgttggta caggaagcct gcgtggaaag atgcagttca acatctgctc
      901 caagatgctt gcggagcagg gaaacgatcc ccatgtcgct gatgatgaac ccgtcgaacg
      961 gccatgcttt catctttggc agctggtctt ccaacaaagc caaccgattt tggtcaaata
     1021 gcatgttcac cgtgcagtac agctttttcc ccgttctttc cttcagggcg ctgacctcgc
     1081 caaggtcagc gtcggtgaag ttgccggcgt tggttctcag tgaaaaggga gaaaaaccca
     1141 gatatgccgc atcggctcca tactccaagg ccaggcgcag cttttcgaga tttccggccg
     1201 ggctcagaag ttcgacgctc aagagagggc ttccttcacc gccctggcta cagcttcggc
     1261 gattgagacg acctccttgg cttgggattg agaagtctct tgggaaggat ggaacggttt
     1321 gagctttcgc tttgcatcct cggggaaggc aaggcggagg acctcttcga tcgtggcgac
     1381 gctgtggaac accacccctt tcttcacctc atcttccagt tcatccagat cccgcttgtt
     1441 gaattgcgga atgatgatct ccttgatctt gttccgcttg gcggccagaa tcttctcttt
     1501 caatcccccg atcggcatga ctttcccttt cagggtgagc tcgccggtca tcgccaggtt
     1561 cggagccatc acttggttgg tgatcaacga gtacagtgct acggtcatcg tgattcccgc
     1621 cgacggtcca tccttggggg tcgccccttc gggcacgtgg aggtggacgt tgttgtggtc
     1681 gaaccagagg ggatcgatcc tgaactgctc ggcatgggct ttgatgtatg tatgggcgat
     1741 ggaaaccgat tcctgcatca cgtccccgag ctgaccggtg agtttcactt caccttttcc
     1801 gggggtgttc tgcgcttcga tgatcaacgt gtctccgccc atgctcgtcc atgccaggcc
     1861 gagcgccatt cctatcttgt ccgctttgag gatctcgtct tcgacaaaaa ccggctgccc
     1921 aaggtattcg accagatcct cctgtctgag cacgaccgga gtgtccagat cgggttgctc
     1981 caccaatttc agggctactt tccgattgat cttatcaagg gatttctcga agtttctcac
     2041 ccccgcttcc cgagcgtact catcggcgat ttttcggagg atcggggatg tgtatttgat
     2101 ctcctgcttg ttcaggccgt gtttttccaa cgacttggga acgaggtatt tccggccgat
     2161 cgcgagcttc tcatcgctgg tgtagccgct gaggcggatc acctccatcc gatccaggag
     2221 ggggcgggga atcgtttcga gggtgttcgc ggtgacgatg aagagcacct ggcttacatc
     2281 aaagggaata tccaggtagg tgtccctgaa ggcgctgttc tgctcggggt cgagcacttc
     2341 cagaagagcg cttgcaggat ctccctggta ggagatgccc atcttgtcga tttcatcgat
     2401 caggaacacc ggatttttgc tcttggtgat tctcaggccc tgcacgatct tcccgggcat
     2461 cgcaccgata tacgtccggc ggtggccctt gatctcgctc tcgtcgttca tccctccgac
     2521 ggagaaccgg aaatactctt tcttcagcgc ccgggcgatt gaaactccga cgctcgtttt
     2581 tccgactccc ggcggaccga ccagacagat gatcgagcct ttggtgtcct gtttcttctt
     2641 tcgaaccgca agaaactcga gtattcggtc tttgacatcc ttcatcccgt agtggtcctt
     2701 ttccaagatc cttcgggcgc tgtcgattga aaaggtctcc ggtttgggat ctttccaagg
     2761 caggtcgctg atgatctcga ggtaggtgcg ggtgagtgaa tactccgggg aggtactctc
     2821 catcatctca aggcgactca actcccgttc caccgtttcc cgggcttctt cgttcaacgg
     2881 gagcgatttg aactttttcg tgagccgttc gatcaattcg gccttggggt tggtggtaag
     2941 accaagctcc tgctgaatcg acttgagctc ctcacggaga aagtaatcgc gctggttctt
     3001 ttcaagcttc ttgtttaccc gagcctggat cttcgcctgg acctgggcga tattctgttc
     3061 gtttttaata aagaccagca ctttttcgat gcggctccgc actatgagcg tttcaagaat
     3121 atcctgctga tcggtgcggt cgacattgag aatcgatgca atgaagtcgg caagtttgcc
     3181 cggatgatcg atgttgacca tgttcaaccg catctcttcg ctgatgagct ggttgttctt
     3241 cgtcaggctc ttcatctcgt tgagcaacag gcgggtccat gccctaagct cctcgcggtt
     3301 gtcttcgata tcttcgagat aggtgacttc agcgataatg tatttgcctc cgggataaaa
     3361 ctgcttgatc tcaaatcgtt tgagggtcga gatgaatatg ctcattcccc catcggggag
     3421 tttgattttt tttacaatct tggctaccgt tcccaccgta tagagatctt ccgctccgac
     3481 cgtttccgtt tcaacgtcct ctttgactaa cagcaaccct aaaaatccac catgctttac
     3541 cgcctggttg acgatttcga catcgctttt atcggtaatc atcaacggcg tgaacaagcc
     3601 gggaaacacg gggtttccgc ttaccggtag aataaacaag ttgttgggaa gcagttgctc
     3661 gatcggcatc agttcctgtt cgctcatggc gcctccaaat gatcatgcgg cgaaccgagt
     3721 tgcccgcctg catcaaaggt actcaaactt tcctgtaaag gaaaggcacg gctactcctt
     3781 ggacagccac ttcacgccaa ggtagcagaa cggggtatca atgagggcga aaaccacctt
     3841 gaagagccag taggggatga taagggaaaa gagatagctc accgtgaagg tgggggtaag
     3901 cttgtaaaag gcgataaaca tgaagattac cgtatcgatg aactggctgg ttatcgtccc
     3961 gacgttgttt ctcagccaaa gcatcttgct ctttgtaagc tccttaaggg tcataaaaat
     4021 cacaacgtca acatactggc cgattccgaa actgatcagg cttgccagac tcattctcat
     4081 actcattcca aaaacctgtt ggtatggttg ctgtaaatcc caggttgggt ggggctttac
     4141 cgaaacgaag atcgccgtga caacaagcat gagcgcaagc atgatcgtgc tgaagcggac
     4201 gaaatgtgta acctgcttcc gtccgcgcac ttctccgacg atgtcggtga tcagaaagag
     4261 caccggcatg aaaaaaattc cgacggaaac ccggacaccg aaaaggctga ttattttgct
     4321 gccgaccgta tttaccagca ccattcccgt gatatacagg gaaaacaaga aaagatcctt
     4381 ctttgtccac gtttccattg cgtacctcac ttgcgctgag tctatacaaa gttatggtaa
     4441 tggtcaaata cacatcccca tgaaggggta tcttgtgata gaatacctgc acataaggaa
     4501 acaaccatgg aaagcaaact catcgtcatc gccatcgggg gaaactcgct gatcgaggat
     4561 ccaaagcacg ttacggttca aagccaatat gaagcggccc gtaaaaccgc cggccacatc
     4621 gcccgtctgg tccaagccgg tcaccgggtg ctcatcgcac acggcaacgg tccccaagtc
     4681 ggatacgtcc tgcttcgaag cgatatcgcc aaagatgttc tgcaccaggt gccgcttgac
     4741 agctgcgtgg cggacacgca gggagcgatc ggctatcagc ttcaaatggc tctgcacaat
     4801 gagcttgccg agctcggagt gacccgtccg gtcgtgacgg tggtaacgca ggtcgaggtc
     4861 gatgcgaatg atccgtcctt ttacaatccc accaaaccga tcggttcctt cctgacccga
     4921 aatgaagctg aagagcggaa aaccaaggat ggatggaacg tagtcgaaga tgccggacgc
     4981 ggctatcgcc gggtggttcc ttcgcccaag cccaaacgga tcgtcgagct ggattcgatc
     5041 cgttcccttt tggaaaacgg ctcgatcgtc atcgctgcgg ggggtggagg gattccggtg
     5101 atcagggatg aagacggaat gctccgaggg aaggaagcgg tcatcgacaa ggatcttgca
     5161 gcagccctgt tggccaagga actgaacgcc gatctgtttg tcatctcgac ggcggtggaa
     5221 aaagtgtttc tcaactgggg caagcccacg caaaagagct tggacctgat aacctccacc
     5281 gaggcgaaac agtatatcaa agaaggacac ttcgcccccg gatcgatgct tccgaaagtc
     5341 gaggcaatcg ttgatttcgt cgaagttacg aacaatacgg gcttgatcac cgacccggca
     5401 aacatccacg gagcgctcta tcgggacaaa gggacgaaga ttgtcaaagg ctaaaagatt
     5461 ttttcccgat ctgaaatccc tcatcgtctc aacggtcgtc atccttcttc ttctgatttt
     5521 tgccgtcgtt gttaccgacc acaacaacgt ccggcgcctt catcgctatc tgtgggaagc
     5581 ggaagcggct aaagaagcgt gctattcact gatcgaacag cgcctcggct atgccaaggc
     5641 gctcgttcgc atcattgata atcaagtgga taccggcagg gtggaagaag tgatcggtca
     5701 atgggatgga gctgcttccg tagatgaagc aagcgttctt tacaaaaccc tcgacgatga
     5761 gcttgccctt cttcaacgaa aggcggtcga acatgaatca taccgcgcct ggtcgccgta
     5821 ctttgaccgg atgtatctga tagagatgga gttgacaaag gcttcggccc actatcagga
     5881 acgcgctgaa ttttttaatg cgcagaaggg cggatttccg gcacgcttgg ccgcaaaacg
     5941 tctcgatctc gaagatcttc tactgtttga tttcggttct tccctgaaag gccgcccgtg
     6001 aaagatagat cggcagcaga agcgcgatga gaagggtcga atcctgatgg aacggagcgt
     6061 ttaaaagctc caacgcaagc aggacgatca tcgacccgag accgaagagg agaaccttgc
     6121 cctgtttttt gggacggaac gcgacgcaga agagaagagg gttgatgaac aggatgttca
     6181 agttccaata ggtcacgttc atgttggttc caaccatcaa cacgaagagc agggtaccca
     6241 ccagagcaag ggcgataaag agtattccat agagcagcga ggtgaagcgg ggaaggtaca
     6301 agccgaggat gatcagaacc atcgaggtga tcaggccgac gaaaaagagg gcgtcgatcg
     6361 taaggaaatc gtgttttgga ggagaaagcg agccttcatt caagaccgta ttgttgatgc
     6421 cgaaatgctg accgtcagcc aatgtaaaat cgagaaccgc ctgatggagt gtctcgggga
     6481 gatacatctc atcccacagc gtcgtcggtt tgtcgatccg ccagctttgc aggaggttca
     6541 gtccgagccc gagaagggga tcatgagcca tgtaaggaag agcggtactt ctcagagatg
     6601 tgccctcccg gcttttagcc catgctttga attgtccacc ggttgccttg tccaggatgt
     6661 cacggacgcg ggtcgcacag ttgtcatcgt agaaatgata cagatacgtt cgcccggtgg
     6721 ggccgatttc atggtacaga aaatcgatca atgcgaattg agcgcttgga tcgaggttca
     6781 ggtcgacgat ccgaacatct cttccttcgg cgatcgaatc ctcgatccgc cagagagcat
     6841 cactgacgct cactcgatag tacatctttc caaaggcaaa gtcccgatag aacccttctt
     6901 tcgtgtcatc gaagattccc cagtcataca tgatctttcg accgttggtt tgggtcacga
     6961 tcaaggcggt gtggccgaac cacgcataga gatcctcccc gggtcctacc gtcaagagct
     7021 ggatgcgggt cttggcgacc caatcctctt ggttttgggc aagcgacgga ccgaagtttg
     7081 cgtagtaaag cgactcgatg ctgtcatagg gctgctcaat ctccaacggg actactgaga
     7141 gagaaaaaag agcgaaagaa agcaggagga gtgttgttac gccaatcaat cgtttcatga
     7201 ttcgcagtat accagatgaa tgcccatctt ccaactggtt gattttttct ttcccgctct
     7261 ttaccctgat gacatggagc gcaatgtttc ctcccgggca atcgtggtgc acagccaaaa
     7321 acaaagtcag atgaaccgaa gattgacgtt gttgagcgtt gatttcggat tgatcgaagc
     7381 aataagctac ggatcggcca aatcgatgcg ggcgccaaaa gcaaatgtct ttgccaacgc
     7441 taccgtctat ctgtactata atccggttcg cgatcactat acgctcaaag atgtggcgat
     7501 cattgagagc aacgaacatc tgcgctcgga gatcacgctg acctatcggg gactttttat
     7561 ggcggaattg atcatgaaaa cccatggagg ggaaagcgag ctcgaatatg aactgttaag
     7621 ccagggcttg agattcttac agagcggtat tgccgatcgg gtgctgatcc aattcgttct
     7681 gcgccttgcc cggattctcg gggtgcatgc cgattatgaa gcgtgtccgg tatgctccag
     7741 gaaataccgt gatgatgaaa cgcttcggtt caccaccgac ctgctcagtc cctgctgttc
     7801 ggagtgcgga agcgccgatc tttcgttgcc tccgggtgct cgccgctatg tgaagctgac
     7861 ttctaccctc gaatatgggg aaagtctgaa cgttccgctc agcgagacgg ctaccgccag
     7921 aatcaaaggc tatgcccttt catacgccaa gctgctggcc cagggaccgc tgaagacgct
     7981 gcagagcggt cttttgtaac acgtgatgag gaaatgacga tggatgatag agtgtgggtg
     8041 aaggaacctg ttgaaagtga ggcgataaag cgccttctgg aagaatatca attcagcacc
     8101 cttcaggcga cgattttaag tcgccgtgga atcaccgaag cggatgatgt gaagtatttc
     8161 ctggaacagg agctttccta tttgcacaac ccctttctct tcgacgagat ggaggatgtg
     8221 gtagatcgga ttcttgacgc agtgcaggaa gaggagaagg tgagaatctt cggtgatcgg
     8281 gatgtcgacg gaattaccgc aaccgccctg cttgttgaag agctgagaag attgaacgtt
     8341 gatgtttcct attcactgcc aacaggggat gagccctatg gactgcttca aagcggcgtc
     8401 gaacaggcgg catccgatgg cgttaccctt ctgatcacgg tggactgcgg aatcggcaat
     8461 atcgaggaga tcgttctggc gaactctctc ggcatcgatg tcatcgtcct cgatcaccac
     8521 atcgaggggg aggagcttcc cccggctctt gcgatcatca atccgaagat gagcggatcg
     8581 gagtatccct tcgtccatct ggccggagtc ggtgtcgccg ccaaaacgat ctgggcgctg
     8641 aggttcgccc ggacggatct gtatcgccaa gaactcattc tactccacgc ccagccgggg
     8701 aacgacacag tgatcatcca ggcgatgaag gtgaacaacc tgctggtcac cgatcgaatc
     8761 atcgaggaga tcaatcccgg ggtactgaaa accgaacgga gcaaagccct cgcgtttctc
     8821 tcctgcaacg tgccgattct agttctggat gccgccgttg aacacgctca attgagcaaa
     8881 gcgtttggta aaagcgtcga tatccacctg cttgatctgc gaaacgagct ggaaaaggtg
     8941 cttccggcgg taagaaaccg gggcctcttc gctctgaccc agctgagtcg ggcggctcgc
     9001 tacgtcaagg ggggaaggga tgagcttact acgctctact cccttttcat cgcctatgtg
     9061 ctgaagaagc accccgcatt ggacgacgag tatgaacggc tcttggatct ggttgccatc
     9121 gggacggtcg ccgacctgat ggagcttaaa aatgaaaacc tcctgatggt aagacggggt
     9181 ctcaaagtcc tgtccaccgc tcctcgacca agcctggttc cctttcttgc ttccctgggg
     9241 cttttgaaca gacggatttc atcgacggag atctcctggc ggatcgcccc gccgatcaac
     9301 tccaccggcc ggctgggaac tccggcggtc gccttggaga tgctgctcag caccgacccg
     9361 aacgagattg aacggctgac gagcgaggtg cttgcgatga atacggagcg caaacgcctc
     9421 agtgatgaga tctggaacgt cgtccgggga aaagctcagg agagctttga gcggaccggc
     9481 tccaagatgg tcatccttca ggatgaacag atcgagatgg gcgtcgccag ttccctcgct
     9541 tcgcgcttgg tgaaagcgta taatgttccc gctctggtgc tttcctcgag ccgtagcgga
     9601 cggataaagg gttcgatgcg cgccaatgac tcgacgatcc atacccgaaa ctttctggag
     9661 catttcgatc ggcaaggttc ctattttctc tactatggcg gacatgcaaa cgcctcggga
     9721 ttcacgatgg aatcggaccg gcttgaagaa ttgatgaagg cgctccacga atacgtggat
     9781 acgatcgatt gtcccgagga acgagtgaaa gagtatttca tcgatgcaac gatcagcgaa
     9841 gaggagatgc accccgagtt gatccggttg gtagaattct tcgagccgta cggagaaggg
     9901 aatcgacccc tcgtatttga aattcaggga gcggtcgtcg aagagatcgg ctatctgaac
     9961 aacagccaag gcggggaccg ccacgtcaag ttgacgatag gatacggtca ctaccgctgg
    10021 ccggcggtct attggtctgc cgctccgctt gtaggaaagg tgttcgaccg gggcagtgtg
    10081 ctggacatcc tctttcgact tgcccgaaac tactggatgg gaaatgagac gctgcagctg
    10141 aacatcgtgg atgtgaagcc gactaaagct ctgtgatgat ccctatcgtt tttcccaccg
    10201 agataaatgc cttcgcggct cgttcgatat cctcggtcgt atgggatgcc gacagttgaa
    10261 cgcggatgcg tgcttttccc ttgggaacta ccggatagga aaagccgatc acatagattc
    10321 cttcattcag cagcttctcg gcaaattcaa cggctttcgc ctcttcatac accatgacgg
    10381 caacaatggc cgtagtcgcg tcgatcagat cgaaccccgc cgttttcaac aagttcctga
    10441 agtgatcggc gtttgccaga gttcgcttgc gcaaggagtg atcttcctgg agaatggaga
    10501 gcaccttcag cgttcctccg cagatcgccg gagccagggt gttggagaag agataggggc
    10561 gggcccgctg gcgatacagg tcgatgagcg gctttcgtcc gctgatgcat ccgccgcttg
    10621 cccctccgca cgctttgccg aacgtcgtgg tgatgatatc gacctttcct tcaacaccgc
    10681 ataattcaac gcttccccgg cctgtttcgc cgagatatcc ggttgcatgg ctgtcatcga
    10741 ctgcgacaag agcgtcgtag gtctccgcca gagcgcagat ctccttcagc tttgcaacat
    10801 cgccatccat tgaaaagacc ccgtcggtaa tgatcaaccg gcggcgagcg gaagcgctcg
    10861 ctttgagcgc ctgttcaagg gcgttcatat cgctgtgctt gtagcggaag cgcccggctt
    10921 ttgaaagccg gattccgtcg atgatcgagg cgtggttcaa ttcatcgctg atgacggcat
    10981 cctcttcccc cagaagcggt tcaaacagcg cggcgttcgc atcgtagcat gatgaaaaga
    11041 ggatcgtgtc ctcggttcga agaaacgatg aaatcgcttg ttcaagctgc ttgtggagcg
    11101 tttgggttcc gcagatgaat cggaccgacg aaagtccgaa gccgaaggtc tccatcgcgt
    11161 ccgctgccgc tcggatgact cgtttgtcat cgctgaggcc aaggtagttg tttgcacaga
    11221 agtttagaac cgttttgccg ccgacaagga tctccctgcc ttgggagcct tcgatgatcc
    11281 gctcctcctt atagagccct tcttccttca atgaagtgag aaattcggtc agttcccgtt
    11341 ccaatcgact gttcatagtc cctcccgttc ccttattttc ccgatcatgt cttccgtcat
    11401 cgaggcgagg gtgtagcggg gtttgaaatc ccattcgacc cgagccgcat agtcttccat
    11461 ataatccggc caggaatcgg cgatcgcctg gcgaaccgga tcaatcgcat agctgatcgt
    11521 gaaggttgga atgtgggtac ggatatacgc cgcctgctcc tcgggacaga agctcatcgc
    11581 ggcgatattg aacgcattcc ggtggcgaag cttcgccgga tccgcctcca tgatcttgat
    11641 tgcagcttcc acggcatccg gcatgtagat catatcaagg taggtgtccc cgcggaggaa
    11701 gcaggtatag tgctgattcc ggatcgcttc gtagtagatc tccaccgcat aatcggtggt
    11761 tccgcctccc gggggcgtca agctgctgat gatccccgga aagcggattc cccgggtatc
    11821 gacatcccag cggtggtggt aatagtcgca gagcagctcc ccggtcacct tggtgacacc
    11881 gtagatcgaa gtagggcgtt gaatcgtatc ctgaggggtg aatttttttg gggtcgtcgg
    11941 cccgaacgcg ccgatcgaag agggaacaaa gacggcgcac gacctctgtc gggcgacctc
    12001 cagcacggcg atcaggccgt tgatattgag attccacgct tccatggggt cctgttcggc
    12061 acgggcggag agcagggctg ccaaatggta gatcgtatcg atccggtggg tgcgaacgac
    12121 ctcgtcgatc cgtttcccgt cgcgggcatc aagaagataa aacggaccat cttcgatcag
    12181 agggcgattc acgtcgcttc tgatatcagc caatacgacg ttgtcggttc catagcggcg
    12241 gcgacattcg agagcgattt cactgccgag ctgaccgagt gcaccgatga tcaatatgcg
    12301 cttcatttct cctcccttcc gcttgtttct tgacaggaac ggagcgtgct tttacaatct
    12361 gtaagcaggt tcagtatatt gaacagggtt tagtatgtca aactttataa ggagttcccg
    12421 atgcaaaccc ctccgatgat cggtaaaaac attcagcgaa ttcgaacggc tcgcaagctt
    12481 accctcaatg tcttgagtga gcgaagcggc gtctccaaag cgatgctcag tcaaatcgaa
    12541 agcgacaagg tgaatcccac cgttgccacg gtatggaaga tcgccaaagg gctgaatgtc
    12601 gaattgaacg accttttgga aggagttgat gaaccgacaa gggtgttttc gctcaatccc
    12661 gcttccgatg acggatcgaa gctcgagaag cggaaggacg ggatcacgat caaaatcctc
    12721 agccccctgt cttccgtgga agatctggag atgtatctcc ttaccttcga accgaactcg
    12781 acccttgcaa gcgagcctca tgtcgccggt agccaggagt atctgacggt cgtcaaaggc
    12841 tcggtcgatg tccgggcagg ggagaactcc gctcacctca aaccgggaga cttcctgatg
    12901 taccacgccg acatcgaaca ttcgatttcc aatcaaagcg gcaaggaagc gatggttcac
    12961 atggtggtga ggttcagttc cgatatccgg tgattccttt gctccttgac acgatgagcg
    13021 gttttattgc aatcttcatc cataagacga acccagtaat cgggaaaggt ggtgatacga
    13081 aaacatggca tacgtccgag tagacgaaaa cgagccgctc gaaaaatcaa tcaagcgatt
    13141 taagcgcatg gtcgagaaag aggggatcat tcgcgaatac aaacgtcgtg aatactttga
    13201 aaagccctcc accctcctga acaggaaaaa gaaagcactt gctcgcaagc agatgaaaaa
    13261 agcccggaag caatacagct ccaaaggcta ctgataccaa gttggtgtta ccgccggcag
    13321 ctttaaaagt tgccggtttt ctcattatat agcccaattt gttaggtaaa taacaaaaat
    13381 accgtatatt tttacggtta atcaataaat atttctctaa aggcttggaa caaattccca
    13441 aaatgcgcta gaatggcatc caatccggat cgtgccggaa atatcctgta aggagtgttt
    13501 cttatgttga tacttgtcgc agacgccttc gatgcttccc tgcccggcag gctctccgtt
    13561 tttggagaag tcaccaccga ccacaatcgt ctttccgaag cgaatgtcgt tctggtccgc
    13621 tcgaaaacga aatgtaccaa agagtacatc gacggagccc cgaacctgaa ggtgattatc
    13681 cgcggcggag tgggtgtgga caacatcgac accgaatatg cgctcagcaa aggcatcgtg
    13741 gtacgaaata ctccaacctc ctcctcgatc gccgtagccg aattgacgat ggcgttaatg
    13801 atcagtgttc cctgcaacct gatcgaatac catcagggaa tgaaagaggg taattggctg
    13861 aagaatctca aacgcaccga gctgtacggg aaaaccctct gcctcctcgg tatggggaat
    13921 atcgcctgca aagttgccga gcgggctcag gcattcggaa tgaacgtcgt tgcatatgat
    13981 aagtatgtaa ctacccatca gtttgcaaag atgcttccga gtgccgaaat cgcggttgag
    14041 aacgccgatt atatctctct gcaccttccg ctgaccaatg aaacgacgaa ccttgtcaac
    14101 gaagagctgc tcagccactg caagaaaaag ccggtggtca tcaacaccgg acgggcgctc
    14161 tgcgtggatg ccgactatat ggccaaagcc ttgaaaggcg gttcggtatc ctggtactgc
    14221 accgatgtct acccaaccga tcccccggcg ggcgattatc cgcttcttga ttgcgatcgg
    14281 gttaccttca cccctcatgt cggggcgaac agcgtggaga atctgggacg aatcggtcag
    14341 gaaacattca atatcatcaa agaactgctg gaaggaggaa ttatctgatg gagcgcaaga
    14401 aaaacttttt tgccggcccg agtgtattgc cgcttgaggt gctgaagcag gtacaggagg
    14461 agttggtcga ctacaagggc aacggacttt cgatgattga aaccagtcac cgcggcggga
    14521 tgtttgaaga gatgtacgat gaaacgctcg cgctcttcaa acgcctcatc ggggcgggtg
    14581 atgagttcga tttctacttt ctcggagggg gagccaccct gcaattctcc atggtaccga
    14641 taaactttct gcctcagggt ggaaaggcgg ggtatatcaa cagcggatcg tggtcggaaa
    14701 aagcgctgat cgacgcaaag aaacttggaa atatcgatgt cgtgtgggac gggaaagaga
    14761 gcggctatac gactcttccg aagatggaga ctcttagggt cgatccgagt ttgagctatc
    14821 tctatctttg ttccaacgag acgatcgggg gtattgaata taaagagttt cccaaaaccg
    14881 attcggttcc cctgattgcc gatatgtcca gcgacatctt cagccgtcct ttcgacgtta
    14941 atcaatttgg aatgattttc ggcggagttc aaaagaacct tggtccggcc ggagccgcct
    15001 tcgtgctcta taagaaagaa ctcctggaaa agcagaatcc caatcttcca tcttatcttg
    15061 attacgctct ccatggaaag aacaaggggc tggcgaatac cccgccggtc ttcgccatct
    15121 gggtggtgaa ccttgtactt aaatggatcg agagccacgg cggaatcgag ggaatggcca
    15181 agcgggctga aacaaagagc agcctcatct atgatgtgat cgactcctct tccttctatc
    15241 gcagccccgt cgacccgagc tatcgctcga caatgaacgt ggtgttccgc cttcccggcg
    15301 aggaattgga ggagctgttc atcaaggaat cgaagaaaga ggggatgctc ggcctgaaag
    15361 gacaccgttc ggtgggcgga ctgcgggcaa gcctgtacaa ctcgctcgag cctgccgccg
    15421 ttgaagcgct cgcccaattt atgcgtgagt ttgagacgcg tcatggataa gcttatgaaa
    15481 ataaaaatgg atgagacgaa caaagcgatt cttcggcaac tcatcgacgg acggaagccc
    15541 ttcagtgcaa tcgccgatga gttgaacatc accgaaaaca ccgtccgttc gcgcgtcaat
    15601 aaactgattg atgaagaagt cctccagatc tccggtctga tccgctccga gatgattccc
    15661 ggtctccaag tcgtcttcat gggagtgaaa ctgtcaacgc tcgatctgga acgaaaagca
    15721 aaggagtttt gcacgttgcg cggtgtcatc tcttccgttg tggtgacggg gcgttacgat
    15781 cttatcgtcc aactggttct cagcgaagaa gaggatctga gtctgctcga ttttttcaaa
    15841 gaggagcttt ccaagatgag cgaggttctg gaagtcgaaa cattcgtagt ctatcaatcg
    15901 gcgaactacc gaattccata cgtgctctga gcgaaaccgg gtagaggaat cggggtaggt
    15961 atggtatact tgccccgtct tggtttgatg aatacctttg gattgaggag aagatacccg
    16021 atggcagatt tgtccagtac ttaccttgga ttaccgatca nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn aggagaagat acccgatggc agatttgtcc agtacttacc
    16201 ttggattacc gatcaggaac cccttcgtag cagcttcagg cccgttgacc gcatcactgg
    16261 attccctgaa acgattggaa gatgccggcc ttggcgccgt tgtcctgaaa tcgatcttcg
    16321 aagagcagat cgagcttgac ggtatgaccg ccctcaaaga tgccgaaagc taccttggcc
    16381 ataccgatgg ctatgagttc gtgaaagcca cctcgatgga acaacagatc gatgcatatc
    16441 tgggcttgct tgaaggagcc aagaagagcc ttgccatccc ggtcatcgct tcgatcaact
    16501 gctatcggga tggaacatgg ctggaatatg caaaacgctt catcgatctc ggagcggatg
    16561 cgattgaatt gaactactat gtggttgctg ccgatcctga aacagagggc agggagatgg
    16621 aaaaggagtt tctccaactg gtccgctccg cccggaagtc gattaatgtc ccgcttagtc
    16681 tgaagatcgg gtgcgcctat tcctcattgg cgaatctgct tcgccgcttc gatgaagagg
    16741 aggtggacgg ggtcgttctg ttcaaccgct tctacagtcc cgacatcgat attgagcaca
    16801 tgaagataac ccaagcgcag atgatcagta gcaaagatga atatgccctt accctcaggt
    16861 gggtcggttt gatgagtgaa gaggttcggt acgacctcat cggcgcgacg ggaatccaca
    16921 gcgccgacac cgccgtcaaa ctgcttctcg caggtgcgaa agcggttcaa ctgtgctccg
    16981 tcctcatgaa ggagggggtg gacagtgtcg cgaagatgct cggggatctt gaagtgtgga
    17041 tgaagaagca tggatttaag gatatcgagg cgttccgcgg gaagcttgcc aagaagtaca
    17101 aggcggacgg ttccgagtgg attcgagttc aatacctcaa aacgatgctc gaggcccaaa
    17161 ggatgtaaaa gatggatatc aattcctatc gtgatattgc cccctatcgc ggccaagacg
    17221 tgttggatgc ggtagagcgg gtcaaagctc atgagaaggc aattgcccag tttatcgcta
    17281 tgctggatcc gccgcggacg aatgatgaac gccttgccct tcaggagtcg gtgaaacaca
    17341 tcgtctccct gcttgatcac gtcaccacgt acgaggagtt tcaacgcacg atcaccgccg
    17401 gattctttct tccgaagatc gtggaaaaat cggtaacggc atttacccac agcggagcgg
    17461 agaagcttgc gaatgatcaa gcatacttgt acgtctccaa ccaccgcgat atcattctcg
    17521 attgcgcctt gatcgatctt gcgctcgccc aagcggatca aatgttgatg gagatggcga
    17581 ttggcgacaa cctgctgacg aaccaatttg tcaccgatct cttcaagctg aacgggggga
    17641 tcgtcgtcaa acgtaccctt ccgctgaggg agaaatacct tgagtcatta cgcctttccg
    17701 catacttcgt cgaatcgatc agcgagcgaa accaatcgat ctgggtagcc caaaaaagcg
    17761 ggaggagcaa ggacggaatc gatgaaacaa accccgccat catcaagatg cttcatctgt
    17821 cccagaaaag gaaaggcgtc agtttcagtg aagtgatcaa gctgtctcgc atcgttccgg
    17881 tagcggtcag ctatgagtac gatcctctgg atgtgaataa agcagcggaa caactccgca
    17941 aacaaagcga taaacgtccg tatgaggatg tgatttcgat gaataaaggc ttgaaagggt
    18001 tcaagggtcg aattcatatc gccttcggca caccgttgac cgaggctgag tatcacacac
    18061 ccgaggaagt ggccaatgaa atcgatcgac agattcatct caactacaag ctttggccaa
    18121 ccaattattt cgcgtatgat catgtaaaca acaccacgac attcgccgaa cagtatcgtg
    18181 atttcaatca tgagacattc ttgcgtcgct ttgcattccg tcgagaggag gttcgagact
    18241 ttgcccttaa tgcatatgca aacccggtca gatcattctt atctcagcaa gctcgtctct
    18301 cataaactgt tctgtctttt tctgatcttc ttcgttgttt tcagtgttgg cgggtgcaaa
    18361 ggggaggagt tcgacgtttt gaatgtcgaa tcgtctctct cggcgctgta ctttctccct
    18421 gaaggagcga gtgcctcaat ccttacagtg agctttgaag taacaagcga tgattccgtg
    18481 ttggaggtgc aagtctccac ccccgataag acttcccgct gggtcgtctc ggtaaagcgc
    18541 gataaacagg gacggttcac cgtcggaccg ctatcgatgg ggaagggaaa caccctcacc
    18601 gaagggatgt attcaatggt gattctcaat gaacagggaa agacgatcga ttactccttt
    18661 ccggtccgct caccgaacac caccgtcgat cccctccaat ggggatcatt tgacagtgat
    18721 gcccggacac tggtgatgaa ccggagtgca acgctccaag tggggaatga ccatattgat
    18781 cttgcggaag gggaaagtta catcttcgct ccggacgatc atgagtttta ccttgccttc
    18841 gatcaggggg cgacgatcgt tcgggtcact ctttaggttc gaccgaccga ggtcatcaaa
    18901 tcatccttat tcaagcgaat gacgaaggtt cggccatggg ggcagatcgg ctcttcaagg
    18961 gcaaagacgc ttttcaggag ctccgtcgcg gcggtggagt cgatgcgatc tcccgcttta
    19021 atcgcagcat ggcacgcaac gaccgaataa agcccttttt caatctcgct cggttccccc
    19081 tgcatcgtct gcagaaaggc gatgacttca tgctcgatcg tccgataaag agcgggaacg
    19141 ccgagcagct cccaggcaag gtcttcgacc ttcctgagct tgattcccag gttgagatag
    19201 ctgtcgaggt ttgacatcag gtactcgtcg atcgaccgct ccacctcgaa gacgatggga
    19261 atcatcaggt tttgaattgg ttgttttgct cgtacctgat catacaggat tcgctcatgg
    19321 gcggcgtgtt gatcgaccag aaaaagatcg tcgccctttt gtgcgattaa aaagaggttg
    19381 aacgcctgac cgaggtaggt gaagctctca tcgctttgct ggaaggtcca gatatctgcc
    19441 tcttcatcgg ttcgcttcgt ctcaaaggcg ggctctttca atgcttcccg tgctttggcg
    19501 aaccaggaag gatcgatttg ttccttcggc gtataggcga atgagcgttc accgaccgtt
    19561 gaatgggttc gaagatgatc atttttctct tcaaagagcg tgggggaggg gctctctttt
    19621 ttttcctgga gccggggaat gggccgtctg acctgagagg agatcatccg gaccacccgg
    19681 tggtggattt ccgccttgtt gcgaaggcgt acctcccgtt tggttggatg gatattgaaa
    19741 tcgaccaggc tcggatcgat cgtgatgaag aggtagcagt agggaaacga tccaccgggc
    19801 aggagattgg aatagccgta ggttaccgcc tgaacaaggg catactcatc aaccggtcgc
    19861 ttgtttacga agatcttgat gtgggagcgg tcgctccgcc acaaggcggc ggaggaagct
    19921 acagcataga ggctgaagcg cccgtggtca tcgaatagtt ccgtcagctc ttccttgacg
    19981 acccgttgac tgattgtcag cacgtcgaca acccgatctt tcgccgtggt gaccggcaga
    20041 ttgaggcgaa gagaaccatc gacgatgagc ttgaacgcaa cgttcgggta cgcaagggct
    20101 ttctcctgta aaaccgttcg aaccatcgcg gcttccgtag ccgcccgttt aagaaattgt
    20161 cggcgggcgg gaagttcgcc aaaaaggtct ttcacgatga cgagcgtgcc tgaagcggtc
    20221 gttgatgaaa gaatctctcc acgtttacca ttatcgatcg ttatctcata tccgttggag
    20281 gcgatcgtga tcgagctgac cgcagcgatc gagtaaaggg cttcaccgcg gaagcccatg
    20341 ctggtgatgt gatacaggtc atccaaatct ctgattttgc tcgttgcatg gctttcgccg
    20401 agcaagggca gatcgccctt ggcgattccg ctcccatcat cgaggactcg catctcttcg
    20461 attcccccct cggtcaccga gacggtgatc gaggtggacc ctgcatctag actgttgtcg
    20521 atcaactccc ggatgaccga tgatggacgt tcgatgacct cgccggcggc gatgcgttgc
    20581 gccaccagcg gattcaggcg ggcaatgggc atcctagaag acaccatcga atcgtacctt
    20641 cacactgata tcaacagaag gtttttggtt gttcagaatg atcggcacgt tcaagcgcgt
    20701 ggtaaaggtg aagattttat aggtgtagga agcttcaaga aacgaggagg cccacttgcc
    20761 gtagaagagg ggtttgagcg aaccgccttc aaaggctgtt ttcagcgtac gcaggaatcc
    20821 tacccgactc gctcccaagc tgagatgaaa cacatccgcg gtataggtga ggccgatttg
    20881 actgaagtcg gcgtcctgag tttcaccttt caccgtagcg agagcgaact ccttggtgaa
    20941 cggaacattg agaatcgcat tggcggtgag gtttttgctc ttccaggttc ctgaagcata
    21001 tacatcgata aatccattat gatcgatgtg gtcgtgggct tcggcaatgt cgttaaagag
    21061 cagcaaatga ttttttcctt tcgccaaaga agccatgatc tgaccgttga acggcttatg
    21121 atttaccgtc agtccgacac caagctggtg gttcgggaag aaataggtgg agttcatgtc
    21181 aaagaactct tcgaacttta cacttgggta aaccggcaga tagccgcttg ccaagaggag
    21241 agcggaaaac tgtgtcaggc gagtattgat gatcgggaag gagagctcaa gcaccggata
    21301 gagctcggct ttgaagatcc gggagctttg tgaatccaag atcgccaagg ttcccaatgc
    21361 gatgcccatg ggatacgatc cgccaaaggt gtactccaac acggtcgaac cgagttgact
    21421 tccgctgtca ttgatgatgt tcgtaaatct taaatcgtca aagctggtag taagtttgaa
    21481 gccgccgatc gtgatcgagt tatacaacgc catctttccc gaggggaagg tggaagcgat
    21541 aaagggggcg agctgcgttc ccggataccc gccctcttca atcagcaggt agaaggggct
    21601 tgtcgaataa ccgactttca catgatcgac gatgccgaag acaaaggaaa caatcccaag
    21661 agcggatgga tcaaggttca ggaagttcgt atcgaatgcg gtgaaggaat ccgtttcaac
    21721 atgtacattc agccgaaggg caagggagtt gtaggagatg aacggcttca cttcaaggcg
    21781 gtggcccggc caataggaag cgctttttat cgggaaggtg agggtgtatc cggcttcaat
    21841 gccataggtt cctttggact ggatgggagc aggcgcactg tcccgaaggg ttgtccgggg
    21901 tttggcgaca gcttccttga cgaccggcat tgctttgaca ctgggggaca acacaaccgg
    21961 tttttcagga agagaacgga caagctcttt gatttcttca gcttcttcga cgaattccaa
    22021 tcccacagtc cattggatgg caccgggttt tgccggaatc cgggccggtc tgacaattcg
    22081 ccctgcggcg gcttcaaccg gagcgagggt tcgtttcgcc aatttgggat tggagagttc
    22141 aagctcctgg agcgcttcga tcgtcgttgt cttatgggtg atcaagttcg tggcgcgtac
    22201 tgcgacgtca aacgattgga ctcgttcctc attccgggtc gaagtgacaa acacttcacc
    22261 ggcgcggtca agttcataca aggtcgccgg agtgatgatt gacaaggtac cctgggtcgt
    22321 tttcgcagtt accgatccat cgatcaacaa aagggtagtg cttgtcgcac tgacctcatc
    22381 cagaatcaga atggagtcag ggaaaagcct gatatctcct cggctggttt ccaagatgat
    22441 cggctcgtcg ccgctgacga ggatcgttcc aagctgaaga tcgttgatat cgattgtttc
    22501 gattgagatg ggttttccgt tgggttcata gaggcgcaag gaggcaagat ctccttcaat
    22561 gacgctcacg tactcttgac caaagatgct taacggcgca atgagggtga gtaagattac
    22621 gaggataaag gtctctagtc gtttcatagt ctctcctaaa cgctccggtt agaggaggac
    22681 gatcctcacc acagcgtcga tagtcgatgt cagatgagtg ttttcatcgt atgccaaact
    22741 gactccaagt gaaatcacat atggctttac tttgatgatg gccaatgctt gaatatcaac
    22801 catgcgcaac gccatcagtt catcaaaaaa gcttccatct ctttcgttag gataaagctt
    22861 ttcatacctc aagtccagag aaagaatgtt gaagagaaag cggtcaaaac gaagatttgc
    22921 gaacaatcgg gtgttttcaa acactccgtc ggtaagatcg ctggcaattt tcgcgccgaa
    22981 gaagatttcc tgtttgggcc aggtgaaaga aatcaacgat tcaaatcgag aagatcccgg
    23041 tggaaggatc atctgctgtt cgtgttcgat cgcccattca ttggagaaaa agcttgcgta
    23101 atagttgccg tacaccggag cggtgagcgc aaagttatag gtgaacaacc ttccgagccg
    23161 accgttgatt ccgccccgaa tcccgatggc ctcctgcggg atcgagatat cgaacccttg
    23221 aaagagaaca tcaataaaaa gatcgctttt gatgtttttt ttctcaacaa ggggatgggc
    23281 gaaatccaga ctgaagaagt gccgttcaat cgttcgctca tcgatgactc tcatcccatg
    23341 agcataggaa aaaccgagtt gcagggatga gagaaaagct ttttcataaa agggattgga
    23401 gtagattcgc catgctttca tcgcaggatc aaacagatca tcgacaagaa aatgaaatcc
    23461 gctttggaca ccggtcaagg atgaagcatc gatcatgagg ttcagtccgg gcttggaggt
    23521 caagaggccc acggagcgat cgacatatcc gttgatcaga gctccgtccc cgagggtagg
    23581 accgagaagc ttgccgtagc ggatgtagat cgggtcatag gggtttccga attgaagggc
    23641 ttggatgaac cgagagtagt gatgaaggag ttgaagggca tactccaaag ccgactcttc
    23701 cgcaccccgt tcggggggaa tccaatcgga gaaatcaaac tggaagtcaa aggggtcgaa
    23761 ggtgaatctt cctttcaggg gaaatgaaag cctggtcgag aaagggccat agctcaaggc
    23821 gggcttgatg atcaccccga aggatccgtc agagagggcg acggcctgaa tcaagccgtc
    23881 atcggtggat tcctcgccca aaccatcgag gggattgagg atcgtgattg aggtggaatg
    23941 attatgtacc cggaaatcac cctccttttg aggaggctga gcgacctctt ccgctcccgc
    24001 cgaaaaggga aagaggagga gcatgaaaag caatataact gagtgctttt tccatcgtcc
    24061 gaacattacg tgtaccatat cattaaaccg atgaattgga caggtgttac acactttccg
    24121 gaaagtcgat tacagctgca gtccgtctca tgtcactatc ctattgttgc atttgggtgg
    24181 gcaacgaaac acctatggcc ggcgatccgc tgcagagcat tgttggtggt tgacaaagat
    24241 atgaatgttc atatgttgag aacctgcaag gttgcagtca aaaccggttc ccagttttaa
    24301 aaaaagtcac atatgcaaaa atataagaca gctattgaga aaagaccaag agcaaggcat
    24361 ggtatttaaa attattgcac aatgtgcaaa tatttaaaan nnnnnnnnnn nnnnnnnnnn
    24421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnna ccaagagcaa ggcatggtat ttaaaattat tgcacaatgt
    24541 gcaaatattt aaaatagagg aatcattgag cgaaatgtat atctcaatgc tacagattcc
    24601 cgactcgttc aaaaaataat cgttgtaggt ggaaattctc ctgcatggat gaatgaagaa
    24661 gaaattccga tcatcaatct gtttgacttc ttgctcaatc cggacagtct ggatctctag
    24721 tcatccaaga aatgatgggc tttatagatg tgttcccggg tcaacgggtc gatcttcacc
    24781 aggctcctga cgatcggctt caattgggat cggatattgg tccggtcata gggacacacc
    24841 ggtttgatga ccggaagatc gtatatttca gaaagacgtt ggatctggcg ttcatggatt
    24901 tcgatcatcg gacggatcac ttgaatcttt ccgttgaaaa acggttgaac gggtttcatc
    24961 gtgtcaaatg atcctcgaaa acacaggttg atcagggatg tttcgacaag gtcgtcgagg
    25021 tgatgtccca gcgcgatctt gacgatccct tcgttctccg cccaatcgaa gaggatccgc
    25081 cggcggttcc gactgcacaa atagcagttg aattcccctt tgaaggaggg agggcgctgt
    25141 tcttcgtcaa caatatgaaa ggcataccca agctcggtaa agaacacagc aagcttttcc
    25201 cgaaactctt cgggaatcgg atgctccttc cagttgatca tcaccgcctg aagggtatag
    25261 tcgatcggca gccacttctt ccgcagcgaa agcgccaagg cgagggcaag gctgtccttt
    25321 ccgccgctgg taccaagcag aatcgtatcc ccgtccctga tcatcgagta ccggttgatc
    25381 gccctcccgg tcgctttcac caggcgcatc acccacgggg gaagatcgcc ttcaagaagc
    25441 gtttgatagg ggagtttctc actcatggat atgctcccaa gcatggcgga gcaccgtcgg
    25501 atcgatggaa ggatcggcaa agaggcgaac ggtcctcagc gatgaggtga actccattgc
    25561 ccggcttcgg gtgaagaggg gatcgacatc ctcgaacctc cccaggaacg tttgatgatt
    25621 gacaaccggg atatcggatt caaaggaaac ccgttccggg atatcgatga cgatcgatcc
    25681 gcctatgagg ttcaggtctt tttcaatccg ttcttcatac tcactgcgtt gcctgaggct
    25741 gagggcattg gtgtgaatcg gattcttttc atcgaagagc tcttcgacca ccggttggaa
    25801 aagcctgttt tcccgaaccc gggtcaggag attccccaat ggcctttcgg tttggctgag
    25861 caggaagaag ctctcgtcat caaggccata gagcccgtct tcgtcaaggg tcccccgttt
    25921 gaggttttca tagaggcagc gcttgatcat cgccgttgcg ctccgaacgg atgtgtgcca
    25981 gtacacgttc cggtacatca ggtactttga aaacagaaga tgttcgatcg atccgagcgc
    26041 ctcaaagccg atcgcaagct ctccttcgtg gagatatagg tgatcggtaa tgtaggaaac
    26101 gtcctgaatt ccatagggaa cgccggcaaa gtacgcatcc cggttgaggt agtcgagctt
    26161 gtcgggatca agcgcaccgc ttaagatcga gcgatacagg agcgcttctt cattgttcgt
    26221 cggccgctct tcatcgatga tgtcaccgat gatttttgta tccagagcgg catctttcaa
    26281 tgcagtacgg aggattcggt cgttttcaag cagttccgtc gccaattgtt cgtgcgattt
    26341 gagcggcagt tctttgaggc tgtgagtgaa cgggaaatgt ccgatatcat ggaggagggt
    26401 ggcggcaagg aaatgccgga caccgtcgat cgtgagaaac ccttcatccg cctttcgctt
    26461 cagcagactg ccgatgatct gccgagagag atgaaacact ccgagactgt ggttgagtcg
    26521 ggtatgcacc gctccggggt acaggtgaaa cgcgacgccg agctgcttga ttctccccag
    26581 tttgatgaag ctcgggcttt taaccacctc agccatatag gttggaatcg aaatatcctt
    26641 ccacagcgga tcgcggaagg tctgagcgtg ggtgaagctc gaatcggtga gaaaccgagt
    26701 gtacgtaaga ctcatatacc gccactgtac ttgatgaaat gagtttgtgt aaacctctgt
    26761 cacggagtat actgtatcaa atgaatacct acctgattat cgctgacgat tttacgggtt
    26821 ccaatgacag cgctcttgaa ctggctcatc gggggctctc cacgatcatc ggcttctctt
    26881 cgccgaaagg gacggtagac tcgtatgtcc tggatacgga gtcccgaaac agagaaggtg
    26941 atgaggcgta caggatgacc aaggctctca tcaaagacct tgatatcgac tcatacgaca
    27001 tcgtcctgaa aaaaattgat tcgactcttc gagggaatat tcttgaagag atccgggcga
    27061 ttgacgaaaa aatgaacagt gatcttattc tcgtcgcccc ggcgttcagc cagatgggac
    27121 gaacctgcgt cggaggaacc gtattcgtga actcggtaaa gctcttggat acggagtttg
    27181 cccacgatcc gctcaaaccg gtaacggagg ataatctcat caccctcttt gcttccgagt
    27241 attcgaccat tcacctctcg gtggatgaga tccgaagcaa agcatttccc ctcatcaagg
    27301 atgcacaggt catcgtctgc gacgcggagc atgaagccga tctctttgaa acggtcaaat
    27361 gggcgagggg tcttgataaa cgaattttgt atgtcggcag tgccggcttg gcaggagcgc
    27421 tgatccaata cgaccgtccg aaacagccgg cccttgcaat tctcgcaagc ttgagcaaag
    27481 caagccgtgc gcagcttgac tacgccaaga gtcgaggggt cgccatcctt gccgtggatg
    27541 tcctttcgat cctcaaaggg ggagacttta ctccaatcat cgatcaagga atacaaatcc
    27601 tccaaggggg agaggatctg atcctcaccc tctcttcagc cttggatcgt tccgtattcc
    27661 aacaatcgat cgaggtggga aaagcgttgg gcttgaagga cgctgttgtc ggaacgatga
    27721 taaaggatcg cctctcgaag gtttccttgg aaatcatcaa agcaagcggg gtgaaaaacc
    27781 tctttctcag tggcggtgag accgcattcg ggctgatgca gatgatggga atcaatgaag
    27841 tgaagatcat cggagaggtg gatatcggaa ttcccatcac tctcgtgctt gacggaccct
    27901 atcaggggat gaaaatcgtt acaaaggccg ggggcttcgg gaaggaagat gcactgacct
    27961 atagcctgag acgtttggag gaacggcaat tatgaaaatc tttggcatta ccttgggcga
    28021 tccctgtggg atcggcatcg aagtcaccat aaaagcgttg ctgaaggaac cttcctatca
    28081 aaaagaagcg ctcctcttcg gtcccctttc agtgctggag tattacaaca aacgattgaa
    28141 agcgaacttc accttcaacg tgatcgaaac aatcgaagag ttcaaaatgg ggatgatcaa
    28201 tgtcgtggat ccccatccgg ccaagcttga ggacgtcctt atcggaaagg tgagcgcgtt
    28261 gggcggggaa gtcgcctttg aagcggtgcg aacggcgatc gagtttgccc ggcgaaagga
    28321 gatcagcagc gtaatcaccg ctcccttgaa caaggaagcg cttcatctgg cgggacaccg
    28381 gtttgccggc cacacggaaa tcttcgccca ctacgccaaa ggggattcct acgcaatgct
    28441 cctctccagt cccaagctga cggtcatcca cgtttcaacc catcaaagcc tgagaagcgc
    28501 gtgcgacgcg gtgacgaaga agcgcgttct ggaggtcatc cggcttggtg cggagacgat
    28561 gaagaagttc ggataccctc atccgaggat cgccgtggcg ggcctcaatc cccatgcagg
    28621 ggagaacggg ctcttcggcg atgaggagat tcgacagatc attccggcga tcgaggcggc
    28681 aaaaaaagaa ggccatgatg taaccggccc gatcgctccc gataccgtgt ttctccgggc
    28741 gatcaaaggg gattgggata tcgtggtggt gatgtaccat gaccaagggc acatccccct
    28801 caaactcctg gcgtttgatg acggggtgaa catcaccgtc ggtcttgacg tggtgcggac
    28861 cagtgtcgat catggcacgg cgttcgacat cgccgacaag ctgatcgcca gtgaacagag
    28921 catgctcaag gcgattgagt tgggaaagaa gctctaaagg aaaaagaaga aaaagagata
    28981 acagagatca gggggccgtt cggccccctg cggttgtaaa cgattacttc acatacggta
    29041 tggcgtatgg accatcgctg agataaagaa tcgtcagatc gcgggttcct tcgcgcctgt
    29101 agatatcctc gattgccgct ttgacgacac tggtgtaatc ccgcttgtcg gtaagtttgg
    29161 cggcattgat gcccggaaga cccgcttccc tgcccgcctc gatttgagga gcatagtggt
    29221 agtagtggtc cagtggaatc ttgtcaaagg cggcagccca cttctgcacc tgccactggt
    29281 cgggggtgaa aggccaatcc ggggagtgaa gaagcttcac cagcgccttc gacccatggt
    29341 tcgccaggat cgccaggacc gttttgtagt ttaccgatcc gacaatatct ttcttgtcgg
    29401 tataattggc gatactgatg agatacccgt tttccttcat tgccccaatc gcggcgaacg
    29461 cagctttggc gctctgataa tggttgatgc cgacgaaccc cgcatgggtt acgacgatat
    29521 cggcttcgga ttcgatggga attttcgcat acccccgaat catttcaaaa gcggccaggt
    29581 gagcggcttc catgtctcct gcatagattc ccgtgatctt gaacgcatga tccaacgtaa
    29641 cattcacgat gaaatccgat cccgccattt ttgcaaaggc aagggattct tcgtggaccg
    29701 ggtttccttc aagaagaaga tcacgggaat cgggatggcc catcatcggg gctccatgga
    29761 acaggaacgt acccatttca ctgatcaaac ccggacaaat ggattttcgt cctccgctca
    29821 ctcccgccat gaagtggctt tcaaccaggc cggtgaggat cttcaagtcg ctttgaagat
    29881 acagggagct gatccacact tcacttccgc gctcggtctt tccaaggtag gtcaatccat
    29941 catcgtccaa acagtcatgg ttgaccactc ggaccccttg ttcatagacc catgggtcga
    30001 taaccgcctc cagctcttca tcggtcatcg gtcggtgggt tcccgtcgac acgagggctg
    30061 tgatcctgtc cgccttgaat ccggcatcaa agagcacttg aataatcggt gtcaaaatat
    30121 ttccttcacc tttataggga accggacggg tgttgtcact gatgacgatg acggcggtcg
    30181 catccttgtt gacagcaagt ttggctttgg ctattgtagc caggctttcg ctttcaatcg
    30241 gattgttgag cgcatccatg atcgccttgt cagcgtttgc cagcgccggt ggggctttca
    30301 tggcgtacac agccgttgta tcaggaagaa ttaattcctt tttcatggga tcgattgggt
    30361 taatcacctt cataggcacc tccttgacga gttaaagaag gcgagcttat gcccgccttc
    30421 ccaaagtata ctcatggttc agtgaacatc ctagagttgt tcgccgaggt cttcgatctt
    30481 tccaccgata tcggcgatgg gacgggtcac aaggcctcgc ttgagctcgt acttgtagaa
    30541 gaagtcgacg aggaagggaa ccaggatcgc cgtcacgacg atcgaagcgg ctacctggat
    30601 cgtcgcttta tcgacatagg gagcccatgc agggtcgaca agggcgatcg ccgcgggggt
    30661 cgccagagcg tttcccgccg tggttccgac accggctccg acagcgctgg ttactcgcat
    30721 cctcttgggg atcaggagcc gatagacaaa gtaggagggc actccggtaa tcaacagggt
    30781 taaaagaccc aggagaatac ccggcaatcc cgcctgaccg agggtctgca gactcaatcc
    30841 ggcaccaagg gggaaggaga aggtgaagat cgcgatgaac ataccgggtt tcaggaactc
    30901 gcggaacttt tcatcaaggt tgcccaggat catgccgacg atgatcggaa cgatgacggc
    30961 gatcagggcg acgatcggaa tatttgcgac accggcgatt cccatgaaca tcatctcgaa
    31021 gaacggtccg tcattggaag agatgattgc aatcgcacca acgtcggatt catctccgta
    31081 cttcgaggca agagcggtgt agagtcctcc gtttgagttg gacatcgcgc tgatcatcgc
    31141 cagaggagtg acaccgagga aggcagcctg aggaccggcg acctttgcga aaatgacacc
    31201 aagaaaaaca ccgaagagca ccttgcttgt gttgaggacg acacccttgt ataccgcaac
    31261 accggcggtc ttcaggttga tctgggctcc gctgcagagg aagagcaatg cgatcaacgt
    31321 caacgcgccg ttcttgaaga aggcagtcgt gaaactacca atgttcaaga cgccggggaa
    31381 gaatgtgttt gtgagaacgc ctaataccag cgggacaacc atcaatccac ccgggatttt
    31441 gttgatcgtg tcgagaattg gaacctttcc cattttcata ggtccacctc cttgaaggaa
    31501 aaatgtatat ttatacatta tcacattcct tgagaatgtc cagaaatatt gagataggaa
    31561 acccgaggaa tgtaaccaac acaaaagatg attgtttact tgatggaggg tggatgaaac
    31621 gggtgctttt gtttctcata ctggcagtgt ccgcaccggg cataggagcc gcttcattgg
    31681 aagaggcgtt ttcctataag cttccggtgg atttttacat cggtcaaatc atcgagcccg
    31741 cagaaaacag catcgaggag cagagcctcg aagcgttgaa agaaccctat actccagcct
    31801 gggttgaaac ctatatcccc gagggaatga gacaggggtt tgttcatacc tacgaccatc
    31861 ttctctccag ctatcttcct tctgaggagc tgcaaatcgg taagccggta aaaattggcg
    31921 cacttgtaga aatccccttt agaatgtttt ccccaaagcc gcttatcggt cttctcgtct
    31981 gggtagaaaa tgatgaaggc gatccattcc tgctttcctt gtctatttcg gaatgacatc
    32041 caggaacctt caactcctgc acgacatatc attctccgct tttttcaaaa gagactcaat
    32101 ttggagtagt tgccttgaga ttccggtgtg aattggttat gtttaaggag tgtactatat
    32161 cgattagggc acaaagcaag aaataggaga ataaaaatgc gaacacagtt agagcacttt
    32221 cacaaacgag ctcgacttta tgtccttggt gctgcaatcc tcatcattct ctttgcactt
    32281 tttatcccca atgtaacggc taaagtaatc ttgattatcc ttacaatcat cgcatttgct
    32341 gttttggcaa ttgttgaatg gttaaaaaaa gacgacgtta catccaaaaa agatgatgca
    32401 agacaagcag caaaagcatc agtcgaaaaa cagaagccgg aagaaaccaa acgaaaagct
    32461 gaagaagcta aaaaggcgga agaagcaaag aaagctgaag aggataagaa aaccgaagca
    32521 gcaaaaaaaa caaaagctaa aaaaacggct gcgccggatg acggcgtcac aacccgaacc
    32581 ggacgctact gcgtggagga tgggacgtac tattgttccg aacaccccga tcgaaccgtt
    32641 gaaatggttg taggaaaaag attcccaccg tgccgcggag atggaaaagg acactcggcg
    32701 atttggacac tttccaaata acatggatca gccaagtaaa ccccctcaaa agcgaggggg
    32761 tttcgtatat tcaggcagtt cttcgttttt tcctgaattg gaaaaagaga atgacaacca
    32821 agatgacaaa cccaactgcg tcctggatcg gatccgccgt gatgaggagc agcgctccga
    32881 caaaggcaag gatgcgttca atccatttca tgcccgtgag ggcgaatccc tccgtagcca
    32941 tgccgatcat cgatacccct atgattgcgg tgattgtgac tctcagagcg acaagaggcg
    33001 tggtgtccaa caagaggaga gcggtattgt acacgaagat gtagggaacg atgaatcccg
    33061 ccaaggaaag acggaccgac tgcagaccgg tcttcatcgg gtcgcccccg cttattcccg
    33121 ctgcagcgaa ggatgcaaga gccaccggag gagtgatgtt ggcgaacatc gcgaagtaga
    33181 agacgaacag gtgggctgca agagccggaa tgccgagctt gatcaacgcg ggagcggcca
    33241 tgctcgcggt gatgagatag gcgggaatgg aaggcaggcc catacccaga accatgcaag
    33301 tcatcatcgt aagaaccaag gtaagcatca ggttcgcccg accgagctgg atgattgcat
    33361 ttgccatatt caacccaaag cccgtaagac ttacgatcgc aatgatgatg cccacgactg
    33421 cacaggcgat cgcaacggag accgtggcgg tggttccctc ggcaaatgca tcaagtattt
    33481 gcctgatcgt cattcgcgtt gatttgcggg tttgggaaat gacgatggtc gccacgatcg
    33541 accagaatgc cgaataaatc acggtaagac cgctgaacag caacagatac aaaagaacaa
    33601 cgatcggaat gagaaggtgt ccccgatcct tcaacacgtc acggactttc ggtaattgtt
    33661 ccttcggaag tccttttaaa tcgcgcctgt ttgcccgaag ctggacttga accatgatcc
    33721 cgaggtagta gagcagggcg ggaatagcag cactgatgac gattgttcca tattttacac
    33781 cgagcatctc cgccatgatg aaggcggcgg cgcccatgat cggaggcaac agctgaccgc
    33841 ccacactcgc cgaggattcg accgctccgg cgaactcctt ggagtagccg ctcttcttca
    33901 tcagcggaat cgtgaaagta ccggtggtta ccacgttggc cactgcactg ccgttgatcg
    33961 aaccgagcag accgctggcg atgaccgcaa ccttggcggg gccacctttg gtggagccgg
    34021 cgaaggcgag ggcgaggtcg ttgaagaagc gccccattcc gcatttgttc ataatcgtac
    34081 cgaagaggat gaagaggaag atataggaag cggcgacatc gacgctggtt ccgtagattc
    34141 cttcggtgtt gatgaaggcg tggttgacga tcgccctcca atcgtatccc cggtgcatga
    34201 agattcccgg aaggcttcgg ccgacgagcc cgtacaggat gaagatcagg gaaaggatca
    34261 ccagcggcca cccgctgatt cttctggatg cctcaagaac gagaataatg agcatcgtac
    34321 cgaagatcac atcgatgagg ttcggagagc ccatccggtt gatgaacccg acgtaatcga
    34381 cccatacata caccgccaca gcgattgaag cgagggctaa ggcgatatca taccagggaa
    34441 tcgtttttcg actggatttt tttccaatgg gataaagaaa aaacgtcatg aacagcatca
    34501 ttgcgacgtg gagacttcgg tggaggtggg tcgccggata gccgacggct gcggtaatga
    34561 agtgatagag agcgattgcg atcgagatcc agtagattcc gatgacgagg gcgtatgaat
    34621 cgaatttgcg cgttgtcgat tccttctcaa actgctcgag aatctcttgt tgtttcaatt
    34681 cctgctcttg attcaaattg ttgagggaga ttttgtccat aggcgctgta ttccttccaa
    34741 tgtcaacgtg accgctttgt ggtgggggag gtcccctcct tcaatcaggg ttttgcctcc
    34801 aaccgttatc gaaaccaacg cggtctctga gtggatccaa gcgaaagagg gaaacgtttc
    34861 attgatattc cgcatgacca ccatgccgtt ttcaacggta gtctcccctt tgtcgttggg
    34921 gattcccgct ccaaagccgg cgacccggat cgcatcaagg cggaaggcaa ggtcttcggt
    34981 gaccgtatac tcttcaatcc acggaatatg ttcataggag tgaatccatt cgaacacgag
    35041 atcgtctcct gccttcaccg gcatcgaagc gaggacggtg ccgtgctcct gatcacggac
    35101 ccgaatcacc ggatcctcgc tcatccggat tcggcatgct gctcctccaa gaagaagcag
    35161 caccggaacg agcagcaaca atgccactct tatcttcatt tatttccagc ccatctc
//
LOCUS       DAOSLI010000015        34879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1420_length_34879_cov_27.318688, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000015 DAOSLI010000000
VERSION     DAOSLI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 34879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34879
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1420_length_34879_cov_27.318688"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /gene="tkt"
                     /locus_tag="PLF67_03645"
     CDS             complement(<1..1553)
                     /gene="tkt"
                     /locus_tag="PLF67_03645"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOE89143.1"
                     /translation="MDVKQLANAIRILSMDGVQKANSGHPGAPMGMAEMAEVLFRRVM
                     KHNPKNPRWANRDRFVLSNGHASMLLYSLLHLSGYEVSLDDLKDFRQLHSKTAGHPEY
                     GDLPGIETTTGPLGQGFANAVGMALAEKSLAARFNREGHKVIDHYTYVFLGDGCLMEG
                     ISHEVASLAGVWKLGKLIVLYDDNGISIDGEVKDWFKDDTAKRFEAYGWHVIRDVDGH
                     DIDSVSKAIVGAQSIKDKPSLLMCRTTIGFGSPNKANTAGSHGAPLGSEEVALTRKNL
                     GWKHDEPFFIPEEIYKAWDQSARGAKDEEAWNAVFASYERAYPAEAAELKRRLANELP
                     EGWEKSFDDAIRKWQSEEVKVASRKASQMSLDILGNLLGELIGGSADLSPSNLTAWKG
                     VKDFDPKTGEGTYLRFGVREFGMMAILNGISLHGGFIPYGGTFLMFMEYARNAVRMAA
                     LMKIRSIGVFTHDSIGLGEDGPTHQPVEQMANLRMTPNMVTWRPCDLVETGVAWKMAI
                     KRSDGPTALL"
     gene            complement(1668..2525)
                     /locus_tag="PLF67_03650"
     CDS             complement(1668..2525)
                     /locus_tag="PLF67_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89144.1"
                     /translation="MKRTIFVVLLIVGVSIGLVAQSGSVTAKLTGALGPGSVDLKAEG
                     AYQVKIPMLQGEGPLFEGNNLKVKAALGVSPIAAQVIVDAVLTPIALAEVNLGVAVGS
                     GWAFPLLKMNGLIKAPAEGAAMVDETLKGVYVKPKAGVALQFDTGAIFASKWASVVLR
                     AYQELNFTYYSNAEKDEPWEFELSGELYNSFNYKGEYIVGYKLPLEWVNLVGVQVEHH
                     VYDAFNRKHGGLVDASILVNSPFPIVDGLSLLMAIQFTNFKAQPDVRNRVVDRLRFKR
                     VALIATYSI"
     gene            complement(2598..4085)
                     /locus_tag="PLF67_03655"
     CDS             complement(2598..4085)
                     /locus_tag="PLF67_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOE89145.1"
                     /translation="MNTKRLFVVVSVLVIAAGLLGAGDGSLAIIHMNDTHSFLLAGRD
                     EHTGREFGGAARWKTIIDELRGDRKDALVLHAGDFLTGSGGDYLSFERIDYDRFPLYG
                     WRGATDVLLLNMLGFDAVTPGNHEFDYGLSWFLSILKPAKFSVLAANLTLNPIPPTKE
                     FKKLSPVKEYEIFTRGGVKVAVIGLVTNDYLKSLQVKVRNAEEVLRTLVPDVRRKADI
                     VVVLSHLGYERDIEVARAVDGIDLIVGGHSHHRLADYTVENGTYITQTGAWGSYVGVI
                     DLHVKNKAIDSIDYRLIPTPNAAEDEEVLSFIEKYKATDLVLDRDLSTEDGSLYQWVL
                     DTVQKRYKTHGAIVIDRLIKEGELKQGVVPSSDFFSIFWPYRARTAGAERDMLATQII
                     DVLSGDHPRSVRSLLSSGDWVSPFVVVTVTQDEIEAIKAFNERNVIAGDHLHMSFEER
                     LPKKPKIALDLQTYRLLILQGVLDPKARHTLAKGELFEVLLESMK"
     gene            complement(4090..4524)
                     /locus_tag="PLF67_03660"
     CDS             complement(4090..4524)
                     /locus_tag="PLF67_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOE89146.1"
                     /translation="MSDNHSYTTNIEWTRNRKGALSSPGLPTMEVATPPNFPGGHPGI
                     WSPEHLFVAAAEVCLMTTFLAIAEKSKLDFLAYRSSATGTLGKLDRGMFVEKIVIRPN
                     VVISDENQREKVITVLEKAKKHCLISKSMVTEVTMEPEVVVR"
     gene            4619..5071
                     /locus_tag="PLF67_03665"
     CDS             4619..5071
                     /locus_tag="PLF67_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOE89147.1"
                     /translation="MIIDSLSNLGRYIPTVPALRLVREILQEDLLSKDLGSYKTSDPN
                     LRYIIMTYRTEKEEADLYEVHRKETDVQILLQGTEVMEIGSRESFRETVAYDEVKDAA
                     FGPADSILTYRADNDHFALFFPGEPHVPNLIDKEQQHIIKVVFKIHQE"
     gene            5189..6187
                     /locus_tag="PLF67_03670"
     CDS             5189..6187
                     /locus_tag="PLF67_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HOE89148.1"
                     /translation="MKKVLATLLVVAMVLPMVFAQGAKEAKQVELVYTMTAVPTDAHA
                     GAMMVFKETVERLSGGEIKVLTYDSASLFKQEQEVAAVKSGQADITATAASWLTDGSP
                     WVSMFTAGYIFKSYDHMTKVLNGPVGQEIFERIAKEQGIRPLGAQYLGTRQLNLIDDK
                     EIKTPADLRGVNLRMPNSDSWIFLGKALGANPTPISFSELYMALQTRTVDGQDNPLPS
                     TKNAKFYEVTKSITITNHLVDSVWPAINEKKWQSLTDQQKAWVMEGVKAGIEYCDTTN
                     LKAEAELVDFFKKAGLKVYYADLDAFADHVLAQYLASPYAATWDKAMFDKVVAAGN"
     gene            6250..6780
                     /locus_tag="PLF67_03675"
     CDS             6250..6780
                     /locus_tag="PLF67_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOE89149.1"
                     /translation="MKKILLFLRDVIELYIPIISFTFLFIAFILQVFFRYVVNRPLTW
                     TQDIIVVGFCWTVILGACYTMRRKGQVSFTMLYDQYPPKVAAWARLAGNVLIIVTFAV
                     LVIPSVNYAIFVSFQKTAALRINYFWIFIPFTYFLLSIIGYTIEPVIEDWKIITGKLD
                     DSAERKMDQLMKEVKA"
     gene            6777..8066
                     /locus_tag="PLF67_03680"
     CDS             6777..8066
                     /locus_tag="PLF67_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOE89150.1"
                     /translation="MSFSLLMTLIVFVLIFFLRMPIAIGMISSAVVYLLVKGADLSMV
                     VNQVMNTFFTNYVIIAVPLFIFTANVMNTGKVTDMIYNFATSVMGRVRGALGHVNILG
                     SLLFSGMTGSAIADAAGLGKIEIEAMRDAGYEVEFACAMTAASATIGPTFPPSIPLVI
                     YAMLSSTSVGALFLSGVVPAILLTIFLMIYVAIVSHIRNYPRGEKIVWKVFLKNTLSA
                     IPALLTPVILLIGIYTGVMTPTEAGAIAGLYALLISFFAYRAMGIKDLLEVLKTTIKD
                     IGSTSIMIGAATVISYIVAREQLAQNIGNWILSLTTSKYMFLFIVNVIILILGMFMDT
                     STIQLVFVPIMLPVARALGIDMIHFGVIVTYNMMVGLSTPPFGMLLFITSGITGTPLK
                     DIVREIWWPLVVMLILLFIITYLPELSLFLPRAAGLL"
     gene            8077..9003
                     /locus_tag="PLF67_03685"
     CDS             8077..9003
                     /locus_tag="PLF67_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOE89151.1"
                     /translation="MKDTVYKGCWPTMITPFTSDNKIDYPAVEALTRWYIDRGADGIF
                     AVCQSSEMFFLSEEEKTDLAKAVVEAADGAIKVIASGHTADDHAKQIDQLGKMGETGV
                     DAVVLVSNRLAKEHEGEDLFRKNIEDIFAQLPGVTFGMYECPYPYLRLLDDEFLRWAA
                     TEDKLVFLKDVSCSLEIQRRRVELVKGTNLALFNANTATVLDSWLMGYHGYNGVMANF
                     HIDLYAWLFEHYKEDPVLAREVMDHLTVWAVAEARSYPVNAKYHRNLTGVPMTLVTRT
                     KDPSLLNENGRHEIASLIRLEGQLRARLGLKP"
     gene            9000..9779
                     /locus_tag="PLF67_03690"
     CDS             9000..9779
                     /locus_tag="PLF67_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOE89152.1"
                     /translation="MRYGKNLREKLKSGRAIGGHVFLGDPAISEALAIYGYDFIWIDA
                     EHGAFDRPLILSHVIAANAGGAAAVVRVPAWSMEAVKPVLEMGIDGIIIPQTKGYEDA
                     KAALAECLYPPRGVRGFGPRRAIRYNDLDTGEYLRNAEDEIVRIIQIEHHKAVEEIDR
                     TLTLDGLDALVLGPNDLASSIGLIGEALHPKVLDLSQHAIAQAKKAGVPIGVSIGPNQ
                     EAIDIWRGLGVDYFSCGDDISFLHQGAKATIEAIQKGQRRE"
     gene            9772..10515
                     /locus_tag="PLF67_03695"
     CDS             9772..10515
                     /locus_tag="PLF67_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOE89153.1"
                     /translation="MNEEGSVLDIEHITLSRTNLSMQIADHLEEVILTSPSKVSDKLP
                     SEMNLAKQYNVSRPVVREAIKLLQERGLVTLKTGSGAYITRPETSTVMNAINRIMQVD
                     QISSEELTEVRAILELSGIELAVGKITDEELEEIDSILTQFENKTLPFKVRVSLDEAF
                     HLALAKATRNNLLALFIGTLTSLLRDYMGKGILIEGGIEDAITRHRQIYHALRARDKE
                     AAIQAMREHLKVSSYNVRVFDEQLHKKKK"
     gene            complement(10497..11636)
                     /locus_tag="PLF67_03700"
     CDS             complement(10497..11636)
                     /locus_tag="PLF67_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOE89154.1"
                     /translation="MELLGSLSRYISQQLDNRKVDSIKKEEFRKTLAAALLMPAFEKP
                     TVTSLWVKEHDELRIEKLTWNVGFGCSEGYLLSPSGAKEKLPGILALHDHAGEKYWGK
                     SKITDIGEKLDRSLVEHQKRYYGARAWANELAKRGYAVFVPDLFGFESRRIKVRELPE
                     SLIQGLLTPPLEIVEGGAYWNDDLVLDDSLDSRVAYNALARGLEHVIAKVLMSGGYTW
                     AGYTAAEDLVALDILSEHELVDSSRLGCGGLSLGGLRSVLLSSLSPKIQASFVTGFMT
                     TFKDLVNAKAANHTWIAYAPRLSHLGEFGDLLMAHAPKPLLVQHGKRDSLFTFSEVER
                     SALRIGEHYKDDSEQFVFTVYDEPHLFNEPMQEEAFAFFDHFFFL"
     gene            11763..12830
                     /locus_tag="PLF67_03705"
     CDS             11763..12830
                     /locus_tag="PLF67_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOE89155.1"
                     /translation="MKLLDIFKKLVAIDSPSFEEDRVALFVEQKLLASGFSTRYDEAG
                     NLYGYLEGEGDTLLLNTHMDTVDLAVGARVVVEDGIIRSDGTTALGADDKAAVAAVLH
                     ALEQVVSAGRKHPPLVVLFTTAEETGLAGAKQVDRSLLDGVAFGYTFDASGPVGGAII
                     QASYHDRLDVVIGGKAAHAGFKPEDGVSAIKIGSDAIASMRLFRIDEETTANVGSFVA
                     PGATNVINPEATISFEARSLNEEKIDKQINHMIEVLEKKAASAGGTVHTHHHRLYDGY
                     RHQADTPVVKRFIEACEALKIPVELKKTQGGSDANIFNALGIPTLTCSVGYENAHSKE
                     EYIPLVELERLASLIKEMIVR"
     gene            12827..13732
                     /locus_tag="PLF67_03710"
     CDS             12827..13732
                     /locus_tag="PLF67_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOE89156.1"
                     /translation="MKNIVIVGAGAVGALYGQRLNSVCSLSFLVDEKRKERYLRDGIF
                     INGELAPFTYACADEIGVPDLVIVATKNHHLKTVIEEMRPIVGPATTILTLLNGIESE
                     GVLAEAFGWEKILHGFAIGLNSTHVGNRIEFTAEGRIVFGERDNSSTDRVARLSKLFA
                     SAGIDAVVPRDIRLMQWNKFMLNTAYNTISGLLSATYEQLDQKAVKELAYRVCSEVQA
                     VALAEGVTIPDSFVEENHNLVITLGDGKTSMCQDLEAGRVTENEWFAGSVVALGGKHA
                     IPTPICQTLSLLVQAKEAISFMALT"
     gene            complement(13693..14565)
                     /locus_tag="PLF67_03715"
     CDS             complement(13693..14565)
                     /locus_tag="PLF67_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOE89157.1"
                     /translation="MHEYTVIDSNEALAKLRNTWKENNVTTIAMDFEGEYNLHIYGEH
                     LCLIQIFDQTTFYLIDPFEISIPELKRFLEDETLEKIMFDCASDAALVRKNHEITLKK
                     IYDLRIAAKQLGMDGGLSKVLDHYLPDRMRRTSGSKKKHQQTNWLMRPLSREQIQYAL
                     EDVEHLFSLKALIIADLERRGLKEKTQALMESAGLPKGPDRPAWTKYPAYRYLSKEER
                     ILLKHYYLAREHVAKRRNVPAVRIMNKKLVLKMAKEKPQSREEFESYTQRNDLLTALS
                     EAHVKAMKEIASLA"
     gene            complement(14681..15970)
                     /locus_tag="PLF67_03720"
     CDS             complement(14681..15970)
                     /locus_tag="PLF67_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOE89158.1"
                     /translation="MAKQPQSTVKYGPSDRPPLGSWIPMSIQHVFAMFGATILVPILT
                     GLSPSTALFTAGTGTLIYILITRAKVPAFLGSSFAFIPALIAISSSYGVAYAMGGAFA
                     SGIFYVIVALIIKKSGHGWIDKALPPVVIGSVIVVIGLSLAPTAMGMAMNDAAGNYNL
                     LVFFVALVTLGFTIAANLFLKGFFSIIPILIGLVAGYLFSLLVGLVSPAHALIDFTAV
                     RQAAWFGLPTFAVPKFNLVATVTFVVVSLATICEHLGDTITISKVVGEDYLVDPGLHR
                     TLAGDGLATLWASLWGGPPNTTYGENIGVLALTRVYSVWVTGGAAVIAVLLSFFPKFG
                     ALIQTIPSPVLGGISMLLFGIIASSGLRTLVDSGVDYQDKRNLTISSVILVIGIGGGA
                     LSFAMGRDLMFTLEGVALATVVGIILNLILPRNGKHE"
     gene            complement(16156..17985)
                     /locus_tag="PLF67_03725"
     CDS             complement(16156..17985)
                     /locus_tag="PLF67_03725"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOE89159.1"
                     /translation="MTLNDIKHAKLKAWIEEFAALATPDAIVIANGSQEQYDELIQQQ
                     IADGYCVALNPKKKPGSVAYNSDPSDVARVENRTYIASETKDEAGPTNNWIDPVELKK
                     TMTDLYRGSMKGRTLYVIPFSMGPLGSPIAKIGVEITDSAYVVLNMMIMTRVGVKVLE
                     LLGTDGEFVPCLHSVGKPLAKGESDHGKWPCAPLEDKYISHFPETREIWSYGSGYGGN
                     ALLGKKCLALRIASVLARDEGWLAEHMLILKITNPKGKSIFVTGAFPSACGKTNLAML
                     VPTIPGWKVETIGDDIAWMKFGKDGRLYAINPEAGFFGVAPGTSEKSNYNAMKAAEKN
                     TIFTNCALTEDNDVWWEGIGYDAPGKLIDWTNNEWIQEKGNKAQKPAAHPNARFTAPA
                     KQAPSIAPEWEDPKGVPISAILFGGRRPKTIPLVHQSRDWNHGVFLGSIVGSEITAAA
                     LDLKVGTIRRDPFAMLPFCGYHMGDYFNHWIKIGENAPDQSKLPKIFYVNWFRRDDDG
                     KFIWPGFGENSRVLKWVFEATEGTAKSVDTPIGIMPAHGAIDLPEGVTEDQMDELLSV
                     DVDGWLAEVADIKTNHYPKFGDRLPKQLSECLAGLEERLKAAK"
     gene            complement(18106..18639)
                     /locus_tag="PLF67_03730"
     CDS             complement(18106..18639)
                     /locus_tag="PLF67_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOE89160.1"
                     /translation="MSTTPIVSVTKGTIIYEQGQPCTTMFVLQSGTIGLYLNWATPTQ
                     FQLLEISKVGTMFGEMGLFEKDPRNATAIALTDCTFLEISSESFPRFVQEHPDQTINI
                     ILDLSQRFKMALQEIKASQEIIAESLAALKEASEYKKDTFKDRIKRISDYLLDIPEDV
                     PPELYLSLNSRFHGTMY"
     gene            18723..20924
                     /locus_tag="PLF67_03735"
     CDS             18723..20924
                     /locus_tag="PLF67_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOE89161.1"
                     /translation="MVELTVPAGSLQSALHAFSEGGDGVYLGLKRFSARKGATNLTIE
                     ELAKLRTVALKENRKIYLTINTLVTDDELNDVIATLKEVDRIGVDGIIVQDLGIARII
                     RNAFPSLPLHASTQLAVHTVEGVRQLQKWGFERIVLARELTLDEIDDIRKACPDVELK
                     VFVHGALCYAFSGLCMASHYLAGRSANQGSCAQICRTWWSVEEDPSLGPGLSPLGKGP
                     RNAPFFSMSDVNSIEVINRLKAMGIDSLKIEGRMKSPAYAHFSARAYRKALDGEPVDL
                     DPLSITFARTQSTGWLAGYGRSGADFSSRHTSSLNSGSTASHMGVAAGTILDYVDHEI
                     QVALDRPVADRDGVMYLAENRLGLFEPVRFAVKGVGFAEAGTRIWIPLPNDAERPTIG
                     SELRLVSGHNLNVPLISEEIPPYQKPIDLTVILEDSKLTIQSKAGTAQAHVSLQKAHR
                     NQDVVENLATIFSQSDQSLFTLGTLNVVNGSSLKIEEIFLPLSTLKKIRRTFYRSLDQ
                     RFKRYVDQPLDLGDVEKKAWEQLPKRSLLVDGDRVPYLDPTHLRKQVGKGKPLEELLF
                     TDGSFLYFPLAPITFLEERFFEDLTWLIDRASDDGCLERIRFGLNNIAHIRFFEKRRL
                     PVFCDIYLYLANSQSARLIGETRLNLIGGYHWLERLEGDTALWPFEPTFVDPAFQAPL
                     FISRSCFRYDSLGLGCEGCPRSGSWYVTQPDKKAHVIVRECITTLLGEERS"
     gene            complement(20893..21402)
                     /locus_tag="PLF67_03740"
     CDS             complement(20893..21402)
                     /locus_tag="PLF67_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOE89162.1"
                     /translation="MDEYFDLVDRERRSLNRIHRRGTPLEAGTYHNVVSVWTMNRDGR
                     VLLTLRSANRPLMPNLWENTSGWVLTGESSVEAALRELEEETGIEATPEQTTLLGTLL
                     KMQSFVDVYLVRVDIDPKSIRLQKEEAISYRWEDEAGLQVLHEEQLLAFPIAFEFEPF
                     RTALRQAAL"
     gene            complement(21402..22421)
                     /gene="asd"
                     /locus_tag="PLF67_03745"
     CDS             complement(21402..22421)
                     /gene="asd"
                     /locus_tag="PLF67_03745"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOE89163.1"
                     /translation="MEKKINVAVMGATGAVGQVFMWMLADHPWFEVTYATASAARAGL
                     KYASTVHWVMPFEMPEKVKETVVQEFNIDAMKEAGVKIVFSALPAEIAREAEPQLRDN
                     GFYVFSNASSMRYDPNVPILIPEANLDQLDLIKKQGYPEKGFVVTNANCSTTGLAMAL
                     APLMKYGIKSVTMHSYQSVSGAGYPGLSSFDITDNCIPYIGGEEEKVEREIKKILAFD
                     PEVFCYTVRVPVMFGHLEAVWIDTEKDLSIEEVKEAWSAFKLEAEYPSSAEHPVQYGE
                     ANTFPQPKYAFWGKPSGMVVYTGRLKKKNGKIGFILLVNNVVKGAAGGSIQNAEAFVS
                     KYGLV"
     gene            complement(22553..23152)
                     /locus_tag="PLF67_03750"
     CDS             complement(22553..23152)
                     /locus_tag="PLF67_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89164.1"
                     /translation="MKTLIFAIVEEGKAERLMDVAKAAGSLGGTILTGQGSGASGLLS
                     LLGLTNFHREILLTIVDDSIKDSVWEALSTCKTRKVLLTSMPLRSNEGGTKPIESTLS
                     MICVICPSGYGWDVLHAARKVGVGGGTIVEGRGTANPDDIAFHGSYLVPEKQVLFMIA
                     ENEVVDAAIRSMSKVPGLAKPGNGIVFTIPLEQFQRVVR"
     gene            complement(23152..24612)
                     /locus_tag="PLF67_03755"
     CDS             complement(23152..24612)
                     /locus_tag="PLF67_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOE89165.1"
                     /translation="MELLNKLKEISSSIIPILLLVTIMHFFIAPLEEGVLLSFYISGV
                     LVIFGLSLILLGVDLGILPIGEKIGSAVTRTRSNFLIGATGFIAGIVIIIAEPNIAML
                     SEQVSSVNPAINTRVMVAMIAIGVGFYVMVGMLRQVYGIPVIWIYLISYIIVFTLAIF
                     STAEMMAIGFDSGGSATGPLSVPFIMALGIGVARVQKVQKEADSFGYVSLALIGPALA
                     LIILGIVSPGTGTGEGSQATVSQAGGFIALIRPSLQMATQAITPMFILSLLYQVFLLK
                     MPSRQMLRAVVGFVYLYIGLTLFFIGSTGGFIPVGYQIGYLLGQISPYLLLFSGLIIG
                     AITAISESSVWVLVNQVQEITKGHLRRSVMLAAISIGVGLSLFLAVLRIWTGLPFWSF
                     ILPAYVIAITLAFFVPELFVGLSFDSGTVASGPMASTFILAFAIGSSMGLETGNAGDA
                     FGIIAFVSVTPVLVVEILGLLYKRTQAKLKKEENQA"
     gene            24737..24809
                     /locus_tag="PLF67_03760"
     tRNA            24737..24809
                     /locus_tag="PLF67_03760"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24770..24772,aa:Ala,seq:ggc)
     gene            complement(25002..26516)
                     /locus_tag="PLF67_03765"
     CDS             complement(25002..26516)
                     /locus_tag="PLF67_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOE89166.1"
                     /translation="MKRRIVFVFFLTLLTVFVGIGAQPVGEHVPDLVSLDVYSTNAFH
                     GRLEAGGNPGAALLGSYIRHYREQNPHMIMVDAGNALQGTAVSTFFDGKPVIEFFNLA
                     GYDALNIGNHEFDWKAGELKRIFADARAPYLGANIITRNGKPVDFAQPYTIIERDGVS
                     VGIIGLIDPAHFDAIAASSLDNIAFVDAAAVAQFYSSLLRERGVDIIILLAMVPGMTD
                     EHGTAKGNLIELARSVPQADAVIGSLHNNVAAIVDGKPVVLAWRYGEKLGHINLVFDR
                     VSRKVVSSSVELIDVAGNPLGLKEDQAIAAMLASYVDILKEENSKPIGYVDQDLVRGS
                     GETAIGNWIVDAMNESAGSQIAFTNPGGIRADLKAGEITGDAIYKICPFPNTIVTTRM
                     DGALLLRFLEQCAEKRPIPISGLRFGFDRSLPVGSQVVFAELMDGTPILPDETYTVTT
                     NSFLKDGKDGYTVLAENTGWVDSAVLMRDAMTQYVLRYKTIRTTLDGRIQDLTP"
     gene            27243..>28754
                     /locus_tag="PLF67_03770"
     CDS             27243..>28754
                     /locus_tag="PLF67_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOE89167.1"
                     /translation="MKKTIDHGQNELNRGNQRLSLIWKAALTGVAVALVVIAYRIVLG
                     RLESLSFQLYGYAKNNPIFLPLVFIVLGIMGYGVGKLVEKNRSISGSGIPQVKGTILG
                     YFNYHWFNTLWSKFLGGAIAIFAGLSVGREGPSIQLGACVAEGLGNRLCDSRTERNYL
                     IAGGASAGLAAAFNAPLAGVVFAVEELFTYISPLILLVTMVSAIVAGSIARLIFGAHP
                     VLAFPIQDALPLSSYWMICLLGIILGGAGSLYNLVLLKTQKLYKKVSNTRIRVMIPFF
                     LAGLLSLVFPAVLGGGHAAMEYLNLSSGLGFLTVLFLVKFIFSMISFGSGAPGGIFFP
                     LLILGAIIGALFGTFSILYLGLDSAYMNNFIILAMTGFFSAIVRAPLTGIVLLLEMTG
                     SFTSMLPLALVAIIASTTADLLKSTPIYHSLLENQRKEAGSANLPDAGQTTTVEMVVH
                     HGSLASDNRVKDLDLPDNCLLIGIRRDGKEIIPRGDTLIRANDYIIFLINLHEE"
     assembly_gap    28755..28854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29029..30756)
                     /locus_tag="PLF67_03775"
     CDS             complement(29029..30756)
                     /locus_tag="PLF67_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE89168.1"
                     /translation="MEKRNARLTYSLIVIVSLLTVAPVIMLVIGSFSNNLNSFGNFTL
                     KKYAAVYTDPMLIDILGDTAAFVLGSALFATVLAIFLAYLNNRTDIPFKFLFKIVSVM
                     PMMIPHVLFAVSWALLLNPTNGILNMWLTRLFALPKAPFNIYTIPGMIVVEGLLDLPV
                     AYLIISPAMGSFDATLEESSRVFKAGPLKTLFRITLPVLRPAIMASFILCVVRALASF
                     AVPSVLGMPGRIYVLSTFIYRVVSMSLIADYGKAAAIGMSILVISISLIYVYRYYTRA
                     SDKFVTVSSRGFKPSVVELRKGRIPLFIIVLFISALLILLPVVVLFYTSLVPYVMVPG
                     AKAFAAMSWDNWKTVIKDPISVRSLKNSLFLGIAGATVGSFLSVLVAYVIVKVKTKAA
                     GVLESISFLSFSFPGIVIGIGFMWYFVRTPLYATIWALFFGYIATYLPYGIRPMTSAF
                     IQIHGSLEESSLVCGASFTTTLRRIVLPLLVPGFVSAWILLATMFFRELSISVVLSRP
                     GTETLAVQILRFSEDGQWGKLSALGLIMIFVSTAMVLAVNALGAYFKPGAVREKKRVG
                     IKPKLKAAV"
     gene            complement(30766..31848)
                     /locus_tag="PLF67_03780"
     CDS             complement(30766..31848)
                     /locus_tag="PLF67_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89169.1"
                     /translation="MEINVRGLKKYYQSEGKVIKALDGVDLTIEANKIFTLLGPSGCG
                     KTTLLRCIVGLETPDEGEIRIGDTIVWSKEKGLYVPPEKRELGMVFQTYAIWPHMNVF
                     NNVAYPLQNMKLPKDEIADRVTKTLKFVQLEGFENRPSTRLSGGQQQRVALARALVAE
                     PKVILFDEPLSNLDAKLREETRKELRVFLTELQITAVYVTHDRVEALALSDMIGVMNK
                     GKIVELGTPRKIYFDADHRFVADFIGRSNLIPAKLLRQEKLHTIVETPIGEIACHKRT
                     FEKDAELVLCLRPEFINTEKPSDEHTNRVTGIIRQMTFVGEVYEADVRIGDETVLVKL
                     DPETTLVEGDELTLSMKPDHCLLVSR"
     gene            complement(31876..32871)
                     /locus_tag="PLF67_03785"
     CDS             complement(31876..32871)
                     /locus_tag="PLF67_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89170.1"
                     /translation="MRKVLLILGVLLASATMLFSAGQADKAVETGPSKVSMYANITAI
                     EPILERYKAESGVEVEYTRLSTARYVSTILTEVEAKKLVADVVQGPLPVLEILREYGV
                     LSNYEAKAMEGYPDWAKKDGIHLFGIEYVGLIYNKDLVSAAEAPKSYMDLTDPKWKGH
                     IVMPDPGAHATTITWLVGLRENVFKSDAEWYRFLEGLAKNEPMLVASFSPTANPLASG
                     EKKIGVSMPKYIVTKAPAPLDYAKISPMLGSPRAIAITKDAPHRKDAEAFLEFWLGKS
                     AASQLANDVGEYVLAPGVYPPIPGIAETEVIPIRELTDEEIQKWGTEFGKIFALK"
     gene            complement(33141..34223)
                     /locus_tag="PLF67_03790"
     CDS             complement(33141..34223)
                     /locus_tag="PLF67_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE89171.1"
                     /translation="MDNRSNGAMYVPEGSSQPVVPRGTFKVGAIGLDHGHIFGMNKGL
                     QDAGATISKVYDPDPEKVAAFLKAFPEAVAADSKDEILADPEIKLVSSAAIPSSRFGI
                     GMEVLDAGKDFFSDKPPFTSQEQVTEARRKVKETGLRWFVYYSERLHVEAAVFAEQLI
                     NDGAIGRVVEIRGWGPHRLSLFSRPAWFFEKDKYGGILTDIGSHQLEQMLFYTGARDA
                     KCLSSRVGNLNHPEHPGLEDFGDATFITDNGVPCYVNVDWFTPDGLGTWGDGRLLVIG
                     TKGYIELRKYTDVARSNTPGHVILVDEKEERHFEVAGKVGFPYFGRMILDCLNRTETA
                     ISQEHIFRAIELALEAQRKAINITRA"
     gene            complement(34226..34465)
                     /locus_tag="PLF67_03795"
     CDS             complement(34226..34465)
                     /locus_tag="PLF67_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89172.1"
                     /translation="MKAISKEEALEVSGYDGRNALELIYAIYQSAAEKREVELPLDRN
                     SAFYTKEGILRVVPKFFKKTKSVANLSGEITLGRN"
     gene            complement(34523..34738)
                     /locus_tag="PLF67_03800"
     CDS             complement(34523..34738)
                     /locus_tag="PLF67_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89173.1"
                     /translation="MKVSIKDIESCTYENNRYSFRGVGGIIHIRLLSETSIRFEYEFS
                     TAVLDSGLERSFAMVQDEMPSRPVPSR"
BASE COUNT         7985 a         9721 c         9178 g         7895 t
ORIGIN      
        1 agcagggccg tcggtccgtc cgagcgcttg atcgccatct tccaggcgac ccccgtttcg
       61 acgaggtcgc agggccgcca ggtgaccatg ttgggcgtca tcctgaggtt cgccatctgt
      121 tccaccggtt ggtgggtcgg cccgtcttcc ccgaggccga ttgaatcgtg ggtgaacacg
      181 ccgattgaac gaatcttcat cagcgccgcc atccggaccg cgttgcgggc gtactccatg
      241 aacatcagga acgttccgcc ataggggatg aagccgccat ggagggagat tccattcaga
      301 atcgccatca tgccgaactc gcggacacca aagcgaaggt acgttccttc cccggtcttc
      361 ggatcgaagt ccttgactcc cttccaagcg gtaaggttgg agggggaaag atccgccgat
      421 ccgccgatca gctcaccgag gagatttccc aagatatcca gactcatctg gctcgccttg
      481 cggctggcga ccttcacctc ttcgctctgc cacttcctga tcgcatcatc aaagctcttc
      541 tcccaaccct ccggcagttc attggccaag cgccgcttca actccgccgc ttcagcggga
      601 tacgccctct cataggaagc gaacacggcg ttccatgctt cttcatcttt cgctccccgg
      661 gcgctctgat cccatgcttt gtagatttct tcggggatga agaacggttc atcatgcttc
      721 caaccaaggt tcttgcgggt cagagctact tcttcgctcc ccagcggagc cccatggctg
      781 cctgccgtgt tggctttgtt cggagaaccg aagccgatcg tggtccgaca catcaacagc
      841 gacggcttat ccttgatcga ctgggctccg acgatcgcct tggagacgga gtcgatatca
      901 tgaccgtcga cgtctcggat tacgtgccat ccatacgcct cgaagcgctt ggcggtatcg
      961 tctttgaacc agtccttgac ttcgccgtcg atcgagattc cattgtcatc atagagcacg
     1021 atgagttttc cgagtttcca cactccggca agggaggcga cctcatggct gattccctcc
     1081 atcagacatc cgtcgcccaa aaagacatag gtgtaatgat cgattacctt atgcccttcc
     1141 ctgttgaatc gggcagccag actcttctcg gcaagcgcca ttccgaccgc attggcgaac
     1201 ccttggccga gcggcccggt ggtcgtctcg attccgggaa gatcgccata ctcggggtgg
     1261 ccggcggtct tgctgtgcaa ctgtctgaaa tccttcaaat catcaagaga aacctcataa
     1321 ccactcaaat ggagcaacga atagagcagc atcgaagcat gtccgttgga aaggacgaag
     1381 cggtcgcggt tcgcccaccg ggggttcttc ggattatgct tcatcactcg tcggaagagg
     1441 acctccgcca tctccgccat tcccatggga gctcccgggt gaccggagtt cgccttttgt
     1501 actccatcca tgctcaagat tcgaattgca ttggcaagct gttttacgtc catcatatcc
     1561 tccacgtata gttcaacaat aaaaagatac cctacttact caagggtgtc aaaagacagg
     1621 cctgcttccg gctcaagaga caggctccat ccatacattc caagaaatca aatcgaatag
     1681 gtcgcgatca gggccacacg cttgaagcgc agccggtcga cgactcgatt tcggacatcg
     1741 ggctgcgcct tgaagttggt gaattggatc gccataagga gtgaaaggcc atcgacgatc
     1801 ggaaacggac tgtttaccag aatcgaagcg tcgaccaatc ccccgtgctt tcggttgaat
     1861 gcgtcataga cgtggtgttc gacctggacg ccaaccagat tcacccattc aagcggtagc
     1921 ttgtagccga cgatatactc acccttatag ttgaaggagt tgtacagctc gccgctgagc
     1981 tcgaactccc acggttcatc cttctccgcg ttgctgtagt aggtgaagtt cagctcttgg
     2041 tacgcccgca gaaccacgct cgcccacttc gaagcaaaga ttgctcccgt atcgaactga
     2101 agggcgactc ctgcctttgg ttttacatac acccctttaa gggtttcgtc gaccatcgca
     2161 gccccttcag ctggggcttt tatgagtccg ttcatcttta acagcgggaa agcccatccg
     2221 cttccgacgg cgacgcccag attgacctcg gcaagggcga tcggggtcaa aaccgcatcg
     2281 acgatgacct gggcggcgat cggcgagact ccgagggcgg ccttcacttt cagattgttg
     2341 ccctcaaaaa ggggcccttc cccctgaagc atcgggatct ttacctgata ggcgccttcc
     2401 gctttgagat ccacgcttcc cgggccaagc gctccggtaa gctttgccgt tacactgccg
     2461 ctttgggcga cgagaccgat gctcacgcca acgatgagca agaccacgaa tattgttcgc
     2521 ttcataggaa ctcctacaca ttataaaatt gtgttcatca taacacacga tgaaggcaat
     2581 gccatatact caccatacta tttcatcgat tcgagcagca cttcaaagag ttcgcctttt
     2641 gccagggtgt gtctggcctt cggatcaaga accccttgga ggatcaatag tcgatacgtt
     2701 tgaagatcaa gggcgatttt gggctttttc ggcagccgtt cctcaaaact catatgcaga
     2761 tgatcgccgg cgatgacatt ccgctcattg aacgccttga tcgcctcgat ctcatcttgc
     2821 gtcaccgtga ccaccacgaa gggggaaacc cagtccccgc ttgaaagcaa gctcctcacg
     2881 cttctgggat gatctccgga gaggacatcg atgatctggg ttgcgagcat atcccgctcc
     2941 gccccggcgg tccgggcgcg gtacggccag aagatcgaga agaaatcact cgaaggaacg
     3001 actccctgct tcagttctcc ttccttgatc aagcggtcga tgacgatcgc tccatgcgtc
     3061 ttgtagcgtt tttgaacggt atccaagacc cattgataca agctgccgtc ctcggtggaa
     3121 agatcgcgat cgaggacgag gtcggtcgct ttatattttt cgatgaagga aagcacctct
     3181 tcgtcctcgg cggcattcgg agtgggaatc agccggtagt cgatcgaatc gatcgccttg
     3241 tttttcacat gaaggtcaat cactcccaca taactgcccc atgctccggt ctgggtgatg
     3301 taggtaccgt tttcaacagt gtagtcggca agccggtgat gggagtgtcc gccgacgatc
     3361 agatcgattc cgtcgaccgc ccgggccacc tcgatatccc gctcgtaccc caggtgggag
     3421 agcaccacga cgatgtccgc cttcctgcga acgtcgggaa ccagggttct gagcacttct
     3481 tcggcattcc ggacctttac ctgaagggat ttgagatagt cgttggtgac cagtccgatc
     3541 accgcgacct tcaccccgcc gcgggtgaaa atctcatact ccttgaccgg ggagagtttc
     3601 ttgaattcct tggtcggagg aatcgggttc aaggtcagat tcgccgcaag cacggagaac
     3661 ttggcgggct tcagaatcga caagaaccag gaaagcccat agtcgaactc gtgattcccc
     3721 ggtgtcaccg catcgaaacc gagcatgttg agaagaagca catcggtcgc tccccgccat
     3781 ccatagaggg ggaagcggtc ataatcgatc cgttcaaagc tcagataatc gccgccgctt
     3841 ccggtaagaa aatcaccggc atggaggaca agagcatcct tccgatcccc ccgaagctcg
     3901 tcgatgatcg ttttccagcg agccgctcct ccgaactccc gacccgtatg ctcatccctc
     3961 ccggcaagca ggaacgagtg ggtgtcattc atatggatga tcgcaagcga gccgtccccg
     4021 gcgcccagaa gcccggcggc aatgaccaga accgagacaa cgacaaaaag acgttttgta
     4081 ttcatggtct cagcgtacca caacctccgg ctccatcgtt acttccgtga ccatcgattt
     4141 tgagataaga cagtgttttt tcgccttttc gagaactgtg atgactttct cccgttggtt
     4201 ttcatcgctg atcaccacat ttggcctgat gacgatcttc tcgacgaaca ttccccggtc
     4261 aagctttccc agcgttcccg ttgcgctcga tcgataggca aggaaatcaa gcttcgattt
     4321 ttcggcgatc gccagaaacg tcgtcatcag acacacctcg gccgcagcca cgaagaggtg
     4381 ctccggcgac cagattcccg gatgtccgcc ggggaagttc ggcggggtcg ccacctccat
     4441 cgtgggaagc cccggtgaag acaacgctcc cttcctgttt cgggtccact ctatgttcgt
     4501 cgtatagcta tgattgtcac tcatattcat cactccttgc cacaacaata ctcacacttg
     4561 ccatattagg ctagcgcaag aaccctctta ggcggtatgg tggaccaaag gagccgccat
     4621 gatcattgat tcgctatcca atctgggccg ttatatcccc accgttccgg cattgcgcct
     4681 tgtccgggag attctccaag aagaccttct ctccaaggat ctcggatcat acaaaacatc
     4741 cgatccgaat cttcgctaca tcatcatgac ctatcggacg gaaaaggaag aggcggatct
     4801 ctatgaagtc cacaggaagg aaaccgatgt gcagattctt cttcagggca cggaagtgat
     4861 ggagatcgga tcgagggaaa gcttcagaga aacggtggcg tatgatgaag tcaaggatgc
     4921 cgcattcgga ccggctgact cgatcctcac ctaccgggcg gataacgacc acttcgctct
     4981 cttttttccg ggtgaacctc acgtcccgaa cctcatcgac aaggaacaac agcacatcat
     5041 caaagtcgtg ttcaagattc atcaggaata atcctctttt ggcaaaaaat atgtttgaca
     5101 aggggatata ctcatgcaat cataacctgt aagacagctt tacaacttaa caaccttccg
     5161 tcgttgacgg aggtatagga ggaatcgtat gaaaaaggta ttagctaccc tgttagtagt
     5221 cgccatggtg ttgcccatgg tgttcgccca aggtgcaaaa gaggccaagc aggttgaact
     5281 cgtatacacg atgaccgccg tccccaccga tgcgcatgcc ggtgcgatga tggtcttcaa
     5341 ggaaaccgtc gagcgcctca gcggcggtga gatcaaagtc ctcacctatg actccgcttc
     5401 gctcttcaag caggagcagg aagtcgctgc ggtaaagagc ggccaggcgg atataaccgc
     5461 cacggcggca agctggctca ccgatggaag tccctgggta tcgatgttca ccgccgggta
     5521 catcttcaag agctatgacc acatgaccaa agtcctcaac ggacctgttg gccaagagat
     5581 ttttgagcgc atcgcgaaag agcagggaat ccgccccctc ggcgcccagt atctcggaac
     5641 ccgccagctg aacctgatcg atgacaagga aatcaagacc cccgccgacc ttcgcggcgt
     5701 gaaccttcgg atgccgaact ccgattcctg gatcttcctt ggaaaagccc tcggagcgaa
     5761 ccccaccccg atctccttca gcgagctcta tatggccttg cagactcgga ccgtagacgg
     5821 tcaggacaac cctcttccgt ccacgaagaa cgcgaagttc tacgaagtga ccaagtccat
     5881 cacgatcacc aaccacctgg tcgactccgt ctggccggcg atcaacgaga agaagtggca
     5941 gtccctcacc gaccagcaga aagcctgggt catggaagga gtcaaagcgg gaatcgagta
     6001 ctgcgacacc accaacctca aagcggaagc cgaactggtc gacttcttca agaaagccgg
     6061 actgaaggtg tactacgccg acttggacgc ctttgccgac cacgttcttg cccagtacct
     6121 ggcaagcccc tatgccgcca cctgggacaa ggcaatgttc gacaaagtcg ttgccgcggg
     6181 taactgatct gtgaaccaca acccggggtt caagccccgg gtttcttttc atctcgaagg
     6241 actatttaca tgaaaaaaat ccttttattc ctcagagacg tcattgagct ctatattccg
     6301 attatctctt ttacgttcct gttcatagca ttcattcttc aagtgttctt ccggtatgtg
     6361 gtcaaccggc cgctgacgtg gactcaggac atcatcgttg tcggtttctg ttggacggtg
     6421 attcttggag cctgctacac gatgcggcgc aaaggacagg tttcctttac gatgctgtat
     6481 gaccaatatc cgccgaaagt cgccgcatgg gcgcgtctgg cgggtaacgt gctgatcatc
     6541 gttaccttcg ccgtgctcgt catcccctcg gtgaactacg cgatcttcgt aagcttccag
     6601 aaaaccgcag cccttcggat caactatttt tggattttca ttccattcac atatttcctt
     6661 ctttccatca tcggatacac gatcgagccg gtcatcgagg attggaagat catcaccggg
     6721 aagctggatg acagcgccga gcgcaaaatg gatcaattga tgaaggaggt gaaggcatga
     6781 gtttttccct gttgatgacc ctgatcgtct ttgtcctgat cttcttcctt cggatgccga
     6841 tcgccatcgg gatgatcagc agcgccgtgg tctatctgct tgtaaaaggt gccgatctct
     6901 ccatggtggt aaaccaggtg atgaacacct ttttcaccaa ctatgtcatc atcgccgttc
     6961 ccctgttcat cttcaccgcg aacgtgatga acacgggaaa ggtcaccgat atgatctaca
     7021 acttcgccac cagcgtgatg gggcgggtcc gcggagccct cgggcatgtg aacatcctcg
     7081 gctccctcct cttcagcggg atgaccggct cggcgatcgc cgatgcggcg gggctgggaa
     7141 agatcgaaat cgaggcgatg agggacgccg gctatgaggt cgagttcgcc tgcgcgatga
     7201 ccgccgcttc ggcgacgatc ggaccgacct tcccgccttc gattccgctg gtcatctacg
     7261 cgatgctttc atcgaccagc gtcggagcgc tctttctaag cggagtggtt cccgccattt
     7321 tgttgacgat cttcctgatg atctacgtcg cgatcgtctc ccacatccgc aactatcccc
     7381 gcggcgagaa gatcgtctgg aaggtgtttt tgaagaacac cttgtcggcg attcccgccc
     7441 tcctcacccc ggtgattctc ctcatcggaa tctacacggg agtgatgacc cccaccgaag
     7501 cgggtgcgat cgccggcctg tatgcgctgc tcatctcctt cttcgcttat cgggcgatgg
     7561 ggatcaagga cctcttggag gtgttgaaaa cgacgatcaa ggatatcgga tcgaccagca
     7621 tcatgatcgg agcggccacg gtgatcagct acatcgtcgc ccgcgagcag cttgcccaga
     7681 acatcggcaa ctggatcctc agccttacaa ccagcaagta catgttcctc ttcatcgtga
     7741 acgtcatcat cctgattctt ggaatgttca tggatacctc cacgatccag ctggtgttcg
     7801 tccccatcat gcttccggtt gcccgggccc tcggaatcga catgatccac ttcggagtga
     7861 tcgtaacgta caacatgatg gtcggtcttt ccactccgcc cttcggaatg ctgctcttca
     7921 tcaccagcgg aatcaccggc accccgctca aggatatcgt acgcgagatc tggtggccgc
     7981 tcgtggtcat gctgatcctt ctgttcatca tcacctacct ccccgagctc agcctctttt
     8041 tgccccgagc cgcgggattg ttataaggat gctgcaatga aagatacggt atataagggc
     8101 tgttggccca cgatgatcac cccgttcacc tccgacaaca agatcgatta cccggcggtt
     8161 gaagctctga ccaggtggta tatcgaccgg ggagcggacg gaatcttcgc cgtctgccaa
     8221 tccagcgaga tgttcttcct ctccgaagag gagaagacgg accttgccaa ggcggtggtc
     8281 gaggcggccg acggggcgat caaagtgatc gccagcggcc acaccgccga cgaccacgcc
     8341 aagcagatcg accaattggg aaagatgggc gaaacgggag tcgacgcggt ggtgctggtc
     8401 tccaaccgcc tcgccaaaga acatgaggga gaggatctct tccgaaaaaa catcgaagac
     8461 atctttgccc aattgccggg cgtaacgttc ggcatgtacg agtgtcccta cccttatctg
     8521 cgcctccttg atgacgagtt cttaagatgg gcggcaacag aggacaagct ggtcttcctc
     8581 aaggatgtct cctgttcgct ggagatccag aggcggcggg tcgaactcgt caaaggcacg
     8641 aacctggccc tcttcaacgc caacaccgcg acggtcctgg attcgtggct gatggggtat
     8701 cacgggtaca acggagtgat ggcgaacttc cacatcgacc tctacgcgtg gctctttgag
     8761 cactacaagg aggatccggt tctcgcccgc gaggtgatgg atcacctcac cgtgtgggcg
     8821 gtcgccgagg cgcgctcgta tccggtgaac gccaaatacc accgaaacct caccggggtt
     8881 ccgatgaccc tcgtcacccg gaccaaggat ccctcattgc tgaatgagaa cggaaggcat
     8941 gaaatcgcca gcctgatccg gcttgaaggc cagctgaggg cccgtttggg actgaaacca
     9001 tgagatacgg aaaaaacctg cgtgaaaaac tgaagagcgg aagggcgatc ggcggacacg
     9061 tcttcttagg ggatccggcg atcagcgaag cgcttgcgat ctacggctat gatttcatct
     9121 ggatcgatgc cgagcacgga gcgttcgacc gacccctcat cctgagccac gtcatcgccg
     9181 caaacgccgg aggagccgcc gcggtggtgc gggttcccgc ctggtcgatg gaagcggtca
     9241 aaccggttct ggagatggga atcgacggca tcatcattcc ccagaccaag gggtatgaag
     9301 atgcaaaagc ggcgttggcc gagtgcctct atcctcctcg gggggttcga ggtttcggcc
     9361 cgcgaagggc gatccgctac aacgacctcg acacgggtga atacctcagg aacgcggagg
     9421 atgagatcgt ccggatcatc cagatcgagc atcacaaagc ggtcgaggag atcgaccgga
     9481 ctctcaccct cgacgggctt gatgcgctcg ttctgggtcc gaacgacctc gcctcctcaa
     9541 tcggtctgat cggcgaggcg ctccacccaa aagtgcttga cctgtcacag catgcgatcg
     9601 ctcaagcgaa gaaagcgggt gttcccatcg gcgtctcgat cggccccaat caggaggcta
     9661 tcgacatttg gagaggattg ggggtagact acttcagctg cggagatgat atctccttcc
     9721 tccatcaagg ggcgaaagcg acgattgaag cgattcaaaa aggacaacgc cgtgaatgaa
     9781 gaaggttctg tactcgacat tgagcacatt accctttcaa gaacaaacct cagcatgcag
     9841 atcgccgacc accttgagga ggtgattctc acctccccgt cgaaggttag cgacaagctc
     9901 ccaagcgaga tgaatctcgc caagcagtac aatgtcagcc gaccggttgt tcgggaggcg
     9961 atcaagctgc tgcaggaacg cggcctggtc accctgaaaa caggaagcgg ggcgtacatc
    10021 acccgccccg agacctccac ggtgatgaat gcgatcaacc gcatcatgca ggtcgatcag
    10081 atcagcagcg aggaattgac ggaggtccgg gcgattcttg agctttcggg aatcgagctc
    10141 gccgtcggga agatcaccga tgaggagctt gaggagatcg attcaatcct cacccaattc
    10201 gaaaacaaga ccctcccctt caaggtccgg gtcagtctgg acgaggcgtt ccacctcgct
    10261 cttgcaaaag caacgagaaa caatctgttg gcccttttta tcggaaccct tacgtccctt
    10321 cttcgcgatt atatgggcaa gggaatcctc atcgaagggg gaatcgagga tgcgatcacc
    10381 cgccaccggc agatctacca tgccctccgc gcccgggaca aagaagcggc cattcaggcg
    10441 atgcgcgaac acctgaaagt ctcctcgtac aacgtccggg tcttcgatga acagcttcac
    10501 aagaagaaaa aatgatcgaa aaacgcaaac gcttcttcct gcatcggctc attgaagaga
    10561 tgcggttcgt catagacggt aaagacgaat tgttctgaat catccttgta gtgctctccg
    10621 atccgcaaag cgcttcgctc gacttcactg aaggtgaaga gcgaatcccg ttttccgtgt
    10681 tggaccagga ggggtttcgg cgcatgagcc atgagcaggt cgccgaactc gccgaggtga
    10741 ctgagccggg gagcataggc gatccaagta tgattcgccg ctttggcgtt caccagatcc
    10801 ttgaaggtgg tcatgaaccc cgtcacgaag ctcgcttgga ttttcggaga gagcgaactg
    10861 agcagcacgc tcctcagccc tccaaggctc aagcctccgc aaccaaggcg ggagctgtcg
    10921 acaagctcgt gttcggagag gatatccaag gcgaccagat cttcggcggc cgtgtatccc
    10981 gcccatgtgt agcccccgct catcagcact ttcgcaatga catgctccag cccccgggca
    11041 agggcgttat acgcgaccct gctgtcaagg ctgtcatcca agacaaggtc atcgttccaa
    11101 taggctcctc cttcgacgat ctccagcgga ggagtcaaca gaccttgaat gagtgactcg
    11161 ggtaattctc tgaccttgat ccttcggctt tcaaagccga agagatcggg aacgaagacc
    11221 gcatagcccc gtttggccaa ttcgttggcc caggctcgtg cgccatagta ccgtttttga
    11281 tgttcaacca aagatcgatc gagcttctct ccgatatccg tgatcttgct ctttccccag
    11341 tacttctccc cggcgtggtc atggagggca aggattccgg gtagcttttc cttggccccc
    11401 gacggggaga gcagataccc ttcactgcag ccgaatccga cgttccacgt caatttttcg
    11461 atccgaagct catcatgttc ctttacccac aggcttgtca cggtcggctt ttcaaaggcg
    11521 ggcatcagca atgccgctgc cagtgttttt cgaaactcct ctttcttgat actatcaacc
    11581 tttcggttgt cgagctgttg actgatatat cgactcaacg atccaagcaa ctccatcaaa
    11641 caacctccct gcaccttggc ttcacctcaa cgatatcata acctgttgcc aagtggcatg
    11701 aaaccttctc cttcgccctc gcctttcttc tctgtctccc cgctttatgc taagctggcc
    11761 ccatgaaatt gcttgatatc ttcaaaaagc ttgttgccat cgattcgccc tccttcgagg
    11821 aggatcgggt cgcattgttc gtggaacaga agcttctggc ttcggggttc agcacccgct
    11881 atgacgaagc cgggaatctc tacgggtacc ttgaaggaga gggtgacact ctcctgctca
    11941 acacccacat ggatacggtg gacttggccg tcggggctcg cgtggtagtt gaagacggca
    12001 tcatccgaag cgacggaacc accgccctcg gggcggatga caaggccgcc gtggcggcgg
    12061 tgctccatgc cctggaacag gtcgtctcgg caggaagaaa gcatccaccg ctggtggtgc
    12121 tcttcaccac cgccgaagaa acggggcttg ccggggcgaa gcaggtcgat cggtcgctgt
    12181 tggacggtgt tgccttcggg tatacgttcg atgcctccgg tccggtgggc ggggcgatca
    12241 tccaagcctc ctatcacgac cgcttggatg tggtcatcgg cgggaaagcc gcccatgccg
    12301 gcttcaagcc ggaagatgga gtcagcgcga tcaagatcgg ttcggatgcg atcgcttcga
    12361 tgcgcctctt ccgcatcgat gaggagacca cggcgaatgt gggctccttc gtcgcacccg
    12421 gggccaccaa tgtgatcaac ccggaagcga cgataagctt tgaagcaaga agcctgaatg
    12481 aagaaaagat cgacaaacag attaaccata tgatcgaggt tctggaaaag aaggccgcct
    12541 cggcgggggg aacggttcat acccaccacc atcgcctcta tgatggatac cggcatcaag
    12601 ccgacactcc ggtcgtcaag cgcttcattg aagcgtgtga agcattgaag attccggtgg
    12661 aactgaagaa gacgcaaggg ggcagcgatg ccaatatctt caatgccttg gggattccca
    12721 cccttacctg ctctgtcggc tatgagaacg cccactccaa agaggaatat attccgctcg
    12781 ttgagctgga acgcctggca tccctgatca aggagatgat tgtcagatga agaatattgt
    12841 cattgtcgga gcgggggccg tgggagccct ctacggtcag cgcctgaact cggtgtgttc
    12901 gttgtccttc ctggtggatg agaagcgaaa agagcgctac cttcgggacg ggattttcat
    12961 aaacggcgaa ctcgccccgt ttacatatgc atgtgccgat gagatcggcg tgcccgatct
    13021 tgtcatcgtc gccacgaaaa accaccattt aaagaccgtc attgaggaga tgagaccgat
    13081 cgtcggtccc gccacgacga ttctcaccct gctgaacggc atcgaaagcg aaggagtcct
    13141 ggctgaagcg ttcgggtggg agaagattct ccacggcttc gccatcggct tgaactccac
    13201 ccatgtgggc aatcggatcg aattcaccgc tgaagggaga atcgtcttcg gcgaacggga
    13261 caattcctcg acggatcggg tcgctcggct gtcgaaactc ttcgcttcag caggcatcga
    13321 tgcggtcgtg ccgagggaca tccgcctgat gcagtggaac aagttcatgc tcaacaccgc
    13381 gtacaatacg atcagcgggc tgctgtcggc gacctacgaa cagcttgacc agaaggcggt
    13441 gaaagagctc gcctacaggg tgtgttccga agtgcaggcg gtggcgttgg ccgaaggggt
    13501 caccatcccc gattccttcg tcgaggagaa ccacaacctc gtcatcacct tgggcgacgg
    13561 aaagacctcg atgtgccagg atctggaagc gggccgggtg acggaaaacg aatggttcgc
    13621 cggatcggtg gttgccttgg gcggaaaaca tgctatccct accccgatct gccaaaccct
    13681 ttcgcttctt gttcaggcca aggaggcgat ctccttcatg gctttgacgt gagcttccga
    13741 cagcgccgta agcagatcgt ttcgttgcgt gtaggactca aactcttccc ggctctgcgg
    13801 cttctctttc gccatcttca agacgagctt cttgttcatg atgcggaccg ccggcacgtt
    13861 ccttcgcttg gcgacgtgct cgcgcgccaa ataataatgc ttcagcaaaa tccgttcctc
    13921 ctttgagagg tagcggtagg cgggatactt cgtccaggct ggtctatcgg gtccttttgg
    13981 cagcccggcg ctttccatca atgcttgagt tttctccttg agtccccgcc tctcaagatc
    14041 ggcgatgatc agggctttga gggaaaagag atgctccaca tcctccaggg cgtattggat
    14101 ctgttctcgg gaaagcggcc gcatcagcca gttggtctgc tgatgctttt tcttcgatcc
    14161 gctcgtgcgc cgcattcggt cgggaaggta gtgatcgagc accttcgaga gtcccccatc
    14221 cattcccagc tgcttcgcgg cgatcctgag gtcatagatc ttcttcaggg tgatctcatg
    14281 gttctttcga accagagcgg catcgctggc gcaatcgaac atgatctttt ccagcgtttc
    14341 atcttccaga aaccgcttca gttcggggat cgagatctca aagggatcaa tcaggtagaa
    14401 ggtcgtctga tcaaagatct ggatgaggca gaggtgctcg ccgtagatgt gaaggttgta
    14461 ctccccttcg aaatccatcg cgatcgtggt gacgttgttt tccttccatg tgtttctcag
    14521 cttggcaagg gcttcattgc tgtcgattac cgtatactca tgcatcgctc aagagtagca
    14581 gaaaaaccgg cgaaagcata tcagggagcc gatcggctcc ctgatggact taattctttc
    14641 gattgttctt ctttataatg ttccgagtgt tgcatgctcg tcactcatgc ttcccgtttc
    14701 tgggaagaat caggttgagg atgattccca cgacggtcgc caaggcaacc ccctccaagg
    14761 tgaacatgag atccctgccc atcgcgaagg agagggctcc tcccccgatc ccgatgacga
    14821 ggatgacgct gctgatcgtg aggttgcgct tatcctggta atcgacgccg ctgtcgacca
    14881 gcgttctcag gccgctggaa gcaatgatgc cgaagagaag cattgaaatc ccgccgagga
    14941 cgggactggg gatcgtctgg atcagcgctc cgaacttggg gaagaaggag agcaggaccg
    15001 cgatcaccgc agcacctccg gtcacccaca cggagtacac gcgggtgagg gcaagaacac
    15061 cgatattctc accgtaggtg gtgttcggag gcccgcccca cagcgatgcc cacagggtcg
    15121 ccaacccgtc tccggcaagg gtacggtgca ggccggggtc gacgaggtag tcctcgccga
    15181 cgaccttgct gatcgtgatc gtatcaccaa ggtgctcgca gatcgtcgcc agcgaaacga
    15241 ccacgaacgt caccgtggca acaaggttga acttgggaac ggcgaaggtc ggcagaccga
    15301 accacgccgc ttggcggacc gcggtgaaat cgatcagggc atgggcgggg ctgacgaggc
    15361 cgacgagcag ggaaaagaga tatccggcca ccaggccgat gagaatcggg atgatcgaga
    15421 agaacccttt caaaaagaga ttcgccgcga tcgtgaagcc gagggtcacc aatgccacga
    15481 agaacactag gaggttgtag ttgcccgccg catcattcat cgccattccc atcgccgtcg
    15541 gagccaatga aagaccgatg accacgatga cgcttccgat caccaccgga ggaagggctt
    15601 tgtcgatcca tccgtgtccg ctctttttga tgatcagggc cacgatgacg taaaatattc
    15661 cgctggcaaa cgcccctccc atcgcataag ccaccccgta ggaggaggag atcgcgatca
    15721 gggcggggat gaacgcaaaa gaagagccaa ggaaagcggg aaccttggct cgtgttatca
    15781 aaatatagat gagggttccc gttccggcgg taaacagcgc ggtcgacgga gaaagcccgg
    15841 tgagaatcgg aacaaggatt gtcgcgccga acatcgcaaa cacgtgttga atgctcattg
    15901 gaatccatga tccgaggggt ggacggtcac ttggaccata tttgacggta ctctgtggtt
    15961 gttttgccat cgagaagctc ccaagtaaga ggaatgatga cctcaaagaa gtctcccaat
    16021 cctacccgta aagaggatcg ttgtgcaacg cccggaagaa cgttattttc cctccgggcg
    16081 atggggatga tgtggcgtag aatcgaaaaa cggcccccga gtcgagggcc gtcggtagat
    16141 tgtgagaaat gactgttact tcgccgcctt cagccgctct tccagcccgg caagacactc
    16201 acttagctgt ttcggaaggc ggtcgccgaa cttcggatag tggttcgtct tgatgtccgc
    16261 cacttcggca agccagccgt cgacatcgac ggacagcagc tcatccatct gatcttcggt
    16321 aacaccttcg ggaagatcga tcgcaccgtg ggcaggcatg attccgatcg gggtatcaac
    16381 actcttggcg gttccttcgg tagcctcgaa gacccacttc agcacgcggc tgttttcgcc
    16441 gaagcccggc cagatgaact tgccgtcgtc atctctgcgg aaccagttga cgtagaagat
    16501 cttcggaagc ttggattgat cgggagcgtt ctctccgatc ttgatccagt ggttgaagta
    16561 gtcgcccatg tggtatccgc agaatggaag catcgcaaac ggatcgcggc ggatcgttcc
    16621 gaccttcagg tcgagcgcag ctgcggtgat ctccgaaccg acgatcgaac cgaggaatac
    16681 cccgtggttc cagtcgcggc tctggtggac caaaggaatc gtcttcgggc ggcggccgcc
    16741 gaagaggatc gcgctgatcg gcaccccctt cggatcttcc cattcagggg cgatcgaggg
    16801 agcctgcttc gcaggggcgg tgaagcgggc gttcggatgg gcggcgggct tctgagcctt
    16861 gttgcccttc tcctggatcc actcgttgtt cgtccagtcg atcagcttgc cgggagcatc
    16921 gtatccgatt ccttcccacc agacatcgtt gtcctcggtc agggcgcagt tggtgaagat
    16981 cgtgttcttc tcggccgcct tcatcgcgtt gtagttcgac ttctcgctgg tgccgggggc
    17041 gacgccgaag aatccggctt ccgggttgat cgcatagagg cgaccgtcct tgccgaactt
    17101 catccaggcg atatcgtctc cgatcgtctc gaccttccag ccggggatcg tcggaacgag
    17161 catcgcgagg ttggtctttc cacacgcgga ggggaatgcg ccggtaacga agatcgattt
    17221 gcccttgggg ttggtgatct tgagaatcag catatgctcg gcgagccatc cctcatcacg
    17281 ggcgaggacg ctggcgatgc gaagggcgag gcacttcttg ccaagcaacg cgttcccgcc
    17341 gtacccggaa ccgtaggacc agatctcgcg ggtctcgggg aagtgggaga tatacttgtc
    17401 ttcaagtgga gcacagggcc atttgccgtg gtcgctctcg cctttggcaa ggggctttcc
    17461 gacggagtgg agacagggaa cgaactcccc gtcggttccg agcagctcaa gcactttgac
    17521 ccccacccgg gtcatgatca tcatgttcag cacgacgtag gcactgtcgg taatctcgac
    17581 accgatcttg gcgatcgggg agcccaacgg tcccattgag aagggaatga catagagcgt
    17641 tcgtcccttc atgcttcctc gatagagatc cgtcatcgtc ttcttcagtt caacgggatc
    17701 gatccagttg tttgtcggac cggcttcatc ttttgtttca ctggcgatgt aggtacgatt
    17761 ttcaacacgt gcaacgtcgg aaggatcgga gttgtacgcg actgaacccg gtttcttctt
    17821 cgggttaagg gcaacgcagt acccatcagc aatctgctgt tggatcagtt cgtcatactg
    17881 ctcttggctt ccattggcga ttacgatcgc atcgggggtg gccaaagctg caaactcttc
    17941 gatccatgcc ttcagtttcg catgtttaat gtcattgagt gtcatggact tctcctttaa
    18001 aatagggtca atttctcata tcgagagaag ttgtacggct tcaacggtat cgtttttccc
    18061 tctctttagc aagagtaaac agtacactga gatttttttc atgtatcagt acatcgtccc
    18121 gtggaaacgg gagttcaagg aaagatacag ctccggcggc acatcctcgg gaatatccaa
    18181 cagataatca ctgattcgct tgattcgatc cttaaacgta tctttcttgt actcgcttgc
    18241 ttctttcaac gcggcaagcg attcggcgat gatctcctgg cttgctttga tctcctgcag
    18301 cgccatcttg aagcgctggc tcaaatctaa aatgatgttg attgtttggt cgggatgctc
    18361 ttggacaaac cggggaaagc tttcgcttga aatctcaagg aacgtgcaat cggtcagagc
    18421 tatcgccgtc gcattgcggg ggtctttttc aaaaagcccc atttcaccga acatcgtacc
    18481 gacttttgaa atctcgagaa gttggaattg cgtcggcgta gcccagttca ggtataagcc
    18541 gatggttcca ctttgcaaaa cgaacatcgt tgtgcatggc tgtccttgct cgtagatgat
    18601 cgttcccttc gtaactgaaa caattggggt ggtagacata tcatctcctc catctcagac
    18661 gacaagtata tcatagagta ggaacgttga cacgtcccgg ggagaaccgt aggctgtaat
    18721 ccatggtcga actcaccgtt ccagccggtt cattacaaag cgcccttcac gcgttcagtg
    18781 agggaggtga tggagtatac ctcggtctta aaaggttttc agctcgcaaa ggtgcgacaa
    18841 atctcacgat tgaggagctt gccaagcttc gcacagtcgc tctgaaagag aacaggaaga
    18901 tctacctcac gatcaacacc ctggtaaccg atgatgagct caacgacgtc attgccaccc
    18961 tgaaggaggt ggatcggatc ggcgtggacg gaatcatcgt ccaggatctt ggaatagccc
    19021 gaatcatcag gaacgctttc ccctccctcc ccctgcatgc ttccacccag ctggcggttc
    19081 ataccgtcga gggagtgagg cagcttcaga agtggggatt cgagcggatc gtgctggctc
    19141 gcgagctcac gttggatgaa attgacgata ttcgtaaagc ctgcccggat gttgagctga
    19201 aagtctttgt ccacggagcg ctctgctatg cgttcagcgg actgtgcatg gccagccatt
    19261 atcttgccgg gcgatcggca aatcaaggct cctgcgccca gatctgtcga acgtggtgga
    19321 gcgtggagga ggatccatcc ctcgggcccg gcctttcccc gctcggcaag gggccgcgaa
    19381 acgccccctt cttctcaatg agcgatgtaa acagcatcga ggtgattaat cgcctcaagg
    19441 cgatgggaat cgacagtctc aagatcgagg ggcggatgaa aagtccggcg tacgcccact
    19501 tttccgcccg agcctaccga aaagctctcg atggcgaacc ggtggacctc gatccccttt
    19561 ccatcacctt cgctcgaacg caaagtacgg gatggctcgc cgggtacggc cgaagcggag
    19621 cggatttctc ttcccgccat acctcctcct tgaacagtgg aagtaccgcc tcccacatgg
    19681 gtgtcgcggc gggaacaatt cttgattacg tcgatcatga gattcaggtc gcattggaca
    19741 ggccggtcgc cgaccgggac ggggtgatgt accttgcgga aaaccgcctc gggctctttg
    19801 agccggttcg ctttgccgtc aagggagtcg gcttcgccga agcgggaaca cggatttgga
    19861 ttccgcttcc aaacgatgcg gaaaggccga cgatcggctc ggagctccgc ctggtcagcg
    19921 gccacaatct gaacgtcccc ctcatttccg aagagattcc cccctatcag aaaccgatcg
    19981 acctcaccgt catcctggaa gattcgaagc tcaccattca aagcaaggcg ggcaccgccc
    20041 aagcccatgt ctcacttcag aaggcgcacc gaaaccaaga cgtcgtagag aatcttgcga
    20101 cgatcttttc ccaaagcgac caatccctct tcactctggg aacattgaac gtggtgaatg
    20161 ggagttcact aaaaattgaa gagatctttt tgccgctctc gacgcttaag aagatccgcc
    20221 ggaccttcta cagaagcctc gatcaacgct tcaagcgcta cgtcgatcaa ccgcttgatc
    20281 tcggcgatgt cgagaagaaa gcatgggagc agcttcccaa gcggagcctg ctggtcgacg
    20341 gggatcgagt cccctatctg gaccccaccc atctgagaaa gcaggttggc aaagggaaac
    20401 cgttggaaga actgctcttc accgacgggt cgtttctcta ctttccgctc gctccgatca
    20461 cctttcttga agaacggttc tttgaggatc tcacttggct tatcgatcgg gcgagcgatg
    20521 acggatgcct tgagcggatc cggttcgggc tcaacaacat cgcccatatc cggttcttcg
    20581 agaagcggcg gctgccggtc ttctgtgaca tctacctcta cctcgccaac agtcaaagcg
    20641 cccgcttgat cggtgaaacc aggctgaacc tcatcggcgg ctaccattgg cttgaacggc
    20701 ttgaagggga taccgcattg tggccgttcg aaccgacgtt cgtcgatccg gcgttccaag
    20761 ccccgttgtt catcagccga tcctgtttcc gatatgattc attgggtcta gggtgtgaag
    20821 gctgtccgcg ctcgggttcc tggtatgtga ctcagcccga caagaaggcg cacgtgatcg
    20881 tccgggagtg catcacaacg ctgcttggcg aagagcggtc ctgaacggtt caaattcaaa
    20941 ggcgatcgga aacgccagga gctgttcttc atggaggact tgaagacctg cttcatcctc
    21001 ccaccggtag gagatcgctt cctctttttg cagccgtatc gacttcggat cgatgtcgac
    21061 ccggacgagg tagacatcaa caaaggattg catttttaag agcgttccca agagagtcgt
    21121 ctgctccggt gtcgcttcaa tcccggtttc ttcctccagt tcgcggagag cggcctcgac
    21181 actgctttca ccggtaagta cccaaccgga ggtgttttcc cacaagttcg gcatcagagg
    21241 tctgttcgcc gagcgaagag tcaacaatac ccttccgtcc ctgttcatcg tccacacgga
    21301 aaccacattg tggtacgtcc ctgcttccaa gggcgtacca cgcctgtgga ttcgattcaa
    21361 tgatcgtcgt tccctgtcga caaggtcgaa atactcatcc atcacaccag gccgtacttg
    21421 ctgacgaacg cctccgcgtt ttgaatggaa ccgcctgcgg ctcctttcac gacgttgttt
    21481 accagaagaa tgaatccgat ttttccgttc ttcttcttca gccggccggt gtacacgacc
    21541 attccgctgg gctttcccca gaacgcatac ttcggctggg ggaaggtgtt cgcttcaccg
    21601 tactgcacgg ggtgctcggc actggagggg tattcggctt caagcttgaa ggcgctccac
    21661 gcttccttga cctcttcaat tgagagatcc ttctcggtat cgatccacac cgcttcaagg
    21721 tgtccgaaca tcaccgggac tcgaacggtg tagcagaaca cttcgggatc gaaggcaaga
    21781 atcttcttga tttccctctc aaccttctcc tcttcgcccc cgatatacgg tatacagttg
    21841 tcggtgatgt caaaggagga aagaccggga tacccggctc cgctgacact ctggtaggag
    21901 tgcatcgtga ccgacttgat tccgtacttc atcaacgggg cgagagccat tgccaatccg
    21961 gtcgtcgaac agttggcgtt ggtcaccacg aatccttttt cgggatatcc ctgctttttg
    22021 atcagatcca gctgatcaag gttcgcctcg ggaatgagaa tcggaacgtt gggatcgtag
    22081 cgcatcgaag acgcgttgga gaacacataa aagccgttgt cccgcaattg aggctccgct
    22141 tcgcgggcta tttcagccgg tagcgcgctg aaaacaatct tcacccccgc ttccttcatc
    22201 gcatcaatgt tgaactcctg caccactgtt tctttgacct tttcaggcat ctcgaagggc
    22261 atcacccagt gaaccgtgga agcgtatttg agacccgctc gggcagcgga agcggtggcg
    22321 taggtgacct cgaaccaagg gtggtccgcc aacatccaca taaacacctg gccgactgcg
    22381 ccggtggcac ccatgacggc aacgttaatc ttcttttcca tagcttcgac tccttgcgtg
    22441 ataccctagc gatttcttca tttttacgca aggctttatt aacaaataat tatatatttt
    22501 aacaaaatgt taatataata actttttgtt catgatatta gcctaactct tattatcgaa
    22561 ccactcgttg aaactgctca aggggaattg taaagacgat tccgtttccc ggctttgcca
    22621 gtccgggcac ttttgacatc gaacgaatcg ccgcatcgac gacctcattc tcggcgatca
    22681 tgaagagcac ttgtttttcc ggcacaagat aggagccgtg gaaggcgatg tcatcgggat
    22741 ttgccgttcc ccgcccctcg acgatcgtcc ctccgcccac cccgactttg cgggctgcat
    22801 gaagaacgtc ccagccgtat ccgctcgggc aaatgacaca gatcatggaa agggtgcttt
    22861 caatcggctt tgtgccgcct tcattcgatc gcaagggcat tgaggtaaga aggactttgc
    22921 gggttttgca cgtgctcaat gcttcccaca ccgagtcctt tatcgaatcg tcgacgatgg
    22981 tgagaaggat ttcccggtgg aaattcgtca acccgagcaa gcttaaaagc ccgcttgcac
    23041 cactgccctg tccggtgaga atcgtgccgc cgagactgcc ggcagctttt gctacgtcca
    23101 taagccgttc ggcttttcct tcttcgacga tggcgaatat caaggttttc atcacgcttg
    23161 attctcctct tttttcagct tagcttgtgt tctcttgtac agcaagccaa gaatttcgac
    23221 gaccaacacg ggagttacgg aaacgaaggc gataattcca aaggcatcgc ctgcatttcc
    23281 cgtctccaaa cccatcgacg aaccgattgc aaaggcgagg atgaaggtgg aagccatcgg
    23341 accggatgct accgttccgc tgtcaaaaga gagcccgaca aacagctcgg gaacaaagaa
    23401 ggcaagcgta atggcgatta cataggcggg cagaatgaag gaccaaaacg gaagcccggt
    23461 ccaaattctc aagaccgcca aaaagagtga aagacctact ccgattgata tcgcagccaa
    23521 catgacggat ctgcgaagat gtcctttggt aatctcctgg acctgattca ccaataccca
    23581 gaccgaagat tcgctgatcg ctgtgatcgc tccgatgata agaccggaga agaggagcag
    23641 atatggactg atttgtccca ggaggtaccc tatctggtag ccaacgggaa tgaagccgcc
    23701 ggttgaaccg atgaagaaga gggtcaagcc gatatagaga tagacgaagc ccaccacggc
    23761 acgaagcatt tggcgggagg gcatcttcaa aagaaagact tgatacagca ggctgaggat
    23821 gaacatcggg gtgatagcct gggtcgccat ctgcaagctg ggacgaatga gggcgatgaa
    23881 ccctccggcc tggctgaccg tcgcctgtga accttcaccg gtgccggtgc cgggcgatac
    23941 gataccgagg atgatcaggg caagggcggg tccgatgagg gctaaagaaa cgtagccgaa
    24001 gctatccgcc tctttctgta ctttttgaac ccgggcgacg ccgattccca atgccatgat
    24061 gaagggaacg gagagcggac cggtagccga tcccccgctg tcaaagccga tcgccatcat
    24121 ctcggcggtg ctgaagatcg ccaacgtgaa cacgatgatg taactgatca gatagatcca
    24181 gatgacgggg attccgtaga cctgacggag cattcccacc atcacgtaga agccgactcc
    24241 gatggcgatc atcgccacca tgacccgggt gttgatcgcg gggttcaccg atgagacctg
    24301 ttcactcagc atcgcgatgt tcggttcggc gatgatgatg acgataccgg cgatgaagcc
    24361 ggtggccccg ataaggaagt tgctccgagt ccgggtcacc gccgatccga tcttctcgcc
    24421 gatcggcaaa atccccaaat caacaccgag gagaatcaac gaaagaccga agatgacgag
    24481 aacgccgctg atgtaaaaag agaggagtac accttcctcc aaaggagcga tgaagaagtg
    24541 catgatcgtc acaagaagga gaattggaat aatggaggag ctaatctcct tgagcttatt
    24601 caacagttcc atttcgcctc ctcacctctc agcacgatat cgaattagta ctcgttcttt
    24661 aataaaaggt caagcaaatc catcccctct tgacgagcgg cggtgcaatc cgtatcatgc
    24721 caaaggtacg gaccttgggg gtatagctca gttggtagag cgcttgaatg gcattcaaga
    24781 ggtcaggggt tcgactcccc ttacctccaa cagacaaggt agtggatttt gatgaaaagt
    24841 ctgctacctt tttcatttgc caaaacatga aaatattaca catctcggaa tagaggtgtt
    24901 atgatcgatg ggttccaagg tacaccgccg aagccggcat atcggttgaa ccgggccgca
    24961 agcctgcccg actccccaga gagaagtcgg gccttcctgc cttacggggt aaggtcttga
    25021 atgcgaccgt cgagagtcgt tcgtatcgtt ttgtaccgca gaacgtactg agtcatcgca
    25081 tcgcgcatga gaacagcgct atcaacccag ccggtattct ccgccagaac cgtatatcca
    25141 tcttttccgt ctttgaggaa actgtttgtc gtaacggtat acgtttcatc gggaagaatc
    25201 ggagttccgt ccatcagttc ggcaaagacc acctgggagc cgacgggaag ggatctgtca
    25261 aatccaaagc gcaggccgga gatcgggatc ggccgcttct cggcacattg ctccaagaaa
    25321 cgaagcagca gagccccgtc catccgtgtc gtaacgatcg tgtttgggaa gggacaaatc
    25381 ttataaatag cgtctccggt aatctccccg gccttcagat ccgcccggat ccccccggga
    25441 ttcgtgaagg cgatctgact gccggccgat tcgttcattg catcgacgat ccagttgccg
    25501 attgcggttt caccgctgcc gcgaacgagg tcttggtcga cgtagccgat cggtttcgag
    25561 ttttcttcct tcagaatatc aacatacgag gcgagcatcg ctgcaatcgc ttgatcttcc
    25621 ttgaggccga gaggattgcc cgccacatcg atcaactcca ccgaggatga tacgaccttc
    25681 cttgacaccc gatcaaagac gaggttgatg tggccgagct tctctccata gcgccaagcg
    25741 aggacaacgg gttttccgtc gacgatcgca gccacgttgt tgtgaaggga accaatgacc
    25801 gcatccgctt ggggtacact gcgggcgagc tcgatgagat tccccttcgc cgtgccatgt
    25861 tcatcggtca ttccgggaac catcgccagc agaatgatga tatccacccc ccgctcccga
    25921 agaagggatg agtaaaactg tgcaaccgcc gcggcatcga caaaggcaat attatccagc
    25981 gaggaagccg caattgcgtc gaagtgggcg ggatcgatca atccgatgat tccgaccgag
    26041 accccatccc gttcaataat cgtataaggc tgtgcaaagt cgaccggctt tccgttgcgg
    26101 gtaatgatat ttgcacccag atagggagcc cgggcatcag cgaaaatacg tttgagctcg
    26161 cctgctttcc agtcgaactc gtgattgccg atgttcaacg cgtcgtatcc ggccagatta
    26221 aaaaactcga tgacgggctt cccgtcgaag aaggtcgata ctgcggttcc ctgaagggca
    26281 ttaccggcat cgaccatgat catgtgggga ttctgctccc gatagtgccg gatgtaggat
    26341 cccaacagcg cggctccggg gtttcctccc gcttcgagcc gtccgtggaa tgcattggtc
    26401 gaatacacat ccagcgaaac gagatccggc acatgttcgc cgacgggttg agcccctatg
    26461 ccgacaaaga ccgtcaacaa cgtcagaaag aatacgaaca caatccttct tttcatagcg
    26521 tttctccttt attatttcct gtatgagcaa ccctaaaact cgatacgaga gaattgctcc
    26581 cggtctgaag gctggtgatt cctagtgtgc tcgaaagcga catctacccg ctttagtgcc
    26641 gcttcggtgg gtatcaattc gtatgtgaag gcttgatgtc gtcgcataat ctgatgatag
    26701 gtccggtctc tgcgcgatgt caacgaaatc ctactccatc ggagggtgcc ggtgcctcga
    26761 atataagaat tccgagagta atcgaaaata gattattcca tagggggaaa gttgtggttt
    26821 aatggataga agaatacaag aaggagaagg cggagagaaa gcctgccttg gaatggacgg
    26881 tctattgaaa tatgctttga gcttgggact gccatgaacg gggaattgaa aaacactcaa
    26941 tcagaaaatc cgtaccgatt gggatgaaga taaagaagaa tgctatttct ccgttgtaga
    27001 cgtggtaggc gtacggatac ccatctgggc tgccgaatat tggagatgga agcaagttgc
    27061 ctgcaaaatg tagtcaactg aacatgctct ccacagatgc cggagtgtaa aagacaggta
    27121 tctcaagctg ttcgatatga cgtcaagatc tgagtaaaga acctcgtagt caagtcggct
    27181 acaataggta taataagatt gcatcaaaaa ctgatctgat agaattacag gaagactcgg
    27241 agatgaaaaa aacgattgat catggtcaaa acgaattgaa tagaggaaac caacgactat
    27301 cgctcatatg gaaagccgca ctgaccgggg ttgccgtagc cttggtcgtc atcgcatacc
    27361 ggatcgtcct cggcagattg gaatcacttt cttttcagct gtatggatat gcgaagaaca
    27421 atcctatctt cctccctctt gtattcatcg tgctgggcat catgggctat ggcgtcggaa
    27481 aactcgtcga aaagaatcgg tcaatcagcg gcagcggtat tcctcaggta aaaggaacca
    27541 ttctcggcta tttcaactat cattggttca acacgctttg gtccaaattc ctgggagggg
    27601 cgatcgccat ttttgccgga ttgtcagttg gacgcgaagg gccgtcaatc caactcggcg
    27661 cctgtgtcgc cgagggcctt ggcaacagat tgtgtgattc ccgcaccgaa cgcaactatt
    27721 tgattgcagg tggcgcaagc gccggactgg cggccgcatt caatgcccct cttgccggtg
    27781 tggtctttgc ggtggaggag ctgtttacct acatatcgcc gctcatcctg ctcgtcacca
    27841 tggtatcggc catcgtagcg ggatccatcg ccagactgat cttcggcgct catcccgttc
    27901 ttgcttttcc cattcaggac gctcttccct tgagctccta ttggatgatc tgtctgctcg
    27961 gcatcatctt gggaggtgcc ggttcattgt ataacctcgt tctgctgaaa acacaaaagc
    28021 tgtataaaaa agtatccaat acacgaattc gggtgatgat cccatttttc cttgcgggtc
    28081 ttctcagcct cgtattcccc gctgttttag gagggggcca tgccgcgatg gaatacctga
    28141 acctgtcaag cggtctgggg tttctcactg tgctttttct tgtcaaattc atcttttcga
    28201 tgataagttt cggatccgga gccccgggcg gcatcttctt tcccttgctg atcctcggtg
    28261 caatcatcgg agccctcttc ggaacattct ccatcctgta tctggggctt gattcggcat
    28321 atatgaacaa tttcatcatt ttggccatga cgggattctt ttcagcaatc gtgcgcgcac
    28381 cgcttaccgg tatcgtcctc ctgttggaaa tgaccggatc gtttacaagc atgcttcctc
    28441 ttgcgttggt cgccatcatc gcctccacaa ccgcagatct tttaaaaagc acacctattt
    28501 atcattcgct tcttgaaaac cagcgcaagg aagccggttc cgccaacctt cccgacgcgg
    28561 ggcaaacgac caccgttgaa atggttgtgc atcatggctc gcttgcatcc gataaccggg
    28621 taaaggatct tgatcttccg gacaactgcc tcctgatcgg aatccgcagg gatggcaaag
    28681 agatcattcc acgcggagac accctgatcc gagccaacga ttacattatt tttctgataa
    28741 accttcacga ggaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaccct
    28861 gatccgagcc aacgattaca ttatttttct gataaacctt cacgaggaag ccgaccaccg
    28921 agatacgcta gccaagctgt tctcggtaca tgaatagatc tgtaggtaaa gaaaaagagc
    28981 tgccgcttct ctggacagcc ctttgagtat tcatctcaaa ccaaatcgtt ataccgctgc
    29041 cttgagttta ggcttgattc ccaccctctt tttctccctc accgccccgg gtttgaagta
    29101 ggcgccgagg gcgttcaccg cgagaaccat cgcagtggag acgaaaatca tgatcagtcc
    29161 aagggcgctg agcttgcccc actggccatc ctcgctgaag cggaggatct gaaccgccaa
    29221 cgtctcggtt cccggacggg aaagaacgac cgagatggaa agctcccgga aaaacatcgt
    29281 ggccagcaga atccaggcgg aaacaaaccc cggaacaagg agcgggagga cgatgcggcg
    29341 cagcgtcgtc gtgaaggaag ccccgcagac cagcgaggac tcttccaagc tgccatggat
    29401 ctgaatgaag gcgctggtca tcggccggat cccatacggc agataggttg cgatgtatcc
    29461 gaagaagagc gcccagatcg tggcgtagag cggagtccgc acaaaatacc acatgaagcc
    29521 gataccgatg acgattcccg ggaatgagaa ggaaaggaag ctgatcgact ccaagacgcc
    29581 tgcggccttc gtcttcactt tcacgatcac atacgctacg agcacggaga ggaaggaacc
    29641 gacggtagcg cccgctatgc ccaagaagag gctgttcttg agcgatcgca ccgagatcgg
    29701 atccttgatg acggttttcc agttgtccca actcatcgct gcaaacgcct ttgccccggg
    29761 caccatgacg tacggaacaa gcgaggtgta gaacagaaca acgacgggaa gcagaatcaa
    29821 cagggcgctg atgaacagaa cgatgatgaa cagcggaatc cttccctttc tcagttccac
    29881 cactgaaggt ttgaaaccgc ggctgctgac ggtaacgaac ttgtcgcttg ctctggtgta
    29941 gtagcgatag acgtagatca ggcttatgct gatcacgagg atgctcattc cgatcgccgc
    30001 ggcttttccg taatcggcga tcaggctcat cgaaacgacc cgatagatga acgttgacag
    30061 cacgtagatt cgaccgggca ttcccaagac ggaaggaacg gcgaacgatg ccagcgctcg
    30121 aacgacacag agaatgaaag acgccatgat cgccgggcga agaaccggca gcgtgatcct
    30181 gaagagggtt ttaagcggtc cggctttaaa aacccgcgac gactcctcaa gcgtggcatc
    30241 aaacgatccc atcgccggtg agatgatcag ataggcaaca ggcaggtcca acagcccctc
    30301 gacgacaatc atccccggaa tggtgtagat gttgaatgga gcctttggaa gcgcaaagag
    30361 tctcgtcagc cacatattga ggattccatt tgtagggttg agcaacagcg cccagctgac
    30421 agcgaacaac acgtgcggaa tcatcatcgg catcaccgat acgatcttga agagaaactt
    30481 gaacggaata tcggtgcggt tgttcagata cgccagaaag atcgccaaca cggtggcgaa
    30541 cagtgccgaa cccaacacga acgccgcagt gtcccccaga atgtcgatga gcatcgggtc
    30601 ggtataaacg gctgcatatt tcttgagggt aaagtttccg aacgaattca ggttgttcga
    30661 gaagcttccg atgaccagca tgatcaccgg ggccacggtg agcaagctga cgatgacgat
    30721 gagggaatag gtaagacggg cgtttctctt ttccatactg ccctcctacc gtgaaacgag
    30781 aagacaatga tccggtttca tgctcagcgt caattcatct ccctcaacaa gtgtggtttc
    30841 cggatccagc ttcaccagaa cggtttcatc tccgattcgg acatcggctt catagacttc
    30901 gccgacgaac gtcatctgtc ggatgattcc cgttacccgg ttcgtatgtt cgtcgctcgg
    30961 tttttcggtg ttgatgaact ccggtctcag acaaagaacc agctcggcat ccttttcaaa
    31021 ggtccgtttg tggcatgcga tttcaccgat cggagtctcc acgatcgtgt gcagcttctc
    31081 ctggcggagg agcttggccg gaatcaggtt tgacctgccg atgaagtcgg caacaaaccg
    31141 gtgatcagcg tcaaagtaga tcttcctcgg tgtaccaagc tcaacgatct ttcctttgtt
    31201 catcaccccg atcatatcgg aaagggcgag agcttcgact cggtcgtgag taacatagac
    31261 tgcggtgatc tgaagctcgg taaggaatac tctcagttct ttacgggttt cttcccggag
    31321 cttcgcatca aggttcgaca gcggttcatc aaacagaatc accttgggtt cggcgacaag
    31381 ggcccgggcg agcgcaacac gctgctgctg cccgccggag agccgggtgg aggggcggtt
    31441 ttcgaacccc tccaactgaa cgaacttgag ggttttggtg acacgatcgg caatttcatc
    31501 ctttggaagc ttcatgtttt gaagcggata ggcgacattg ttgaagacgt tcatatgcgg
    31561 ccagatcgca taggtctgga agaccatgcc cagctcgcgc ttctcagggg gcacataaag
    31621 tcctttttct tttgaccaaa cgatcgtatc tccgatccgg atctctcctt catcgggagt
    31681 ttccaatccg acgatgcacc gaagcaaggt ggttttccca cacccgctcg gaccaagaag
    31741 cgtaaagatc ttattcgcct caatggtgag gtcgacgcca tcgagcgctt tgatcacttt
    31801 accctcgctt tgatagtact tcttcaatcc cctgacatta atttccatat cgtgtttccg
    31861 ccctcgatgt atttcttact tcaatgcgaa gatcttaccg aactcggtgc cccatttttg
    31921 gatttcctcg tccgtaagct cgcgaatcgg aatcacttcc gtctcggcga ttcccgggat
    31981 cggcggatat actcccggag cgagaacata ttcacccacg tcgtttgcaa gctggcttgc
    32041 cgcgctcttg cccagccaga actccaggaa cgcttcggcg tccttcctgt gcggagcatc
    32101 ctttgtgatc gctatggctc ggggcgaacc cagcatcgga gagattttcg catagtcaag
    32161 cggtgccgga gctttcgtca cgatgtattt cggcatggag acgccgatct tcttctcgcc
    32221 gctggcaagc ggattggcgg tcgggctgaa cgaagcgacg agcatcggct cgtttttggc
    32281 caacccttca aggaaccgat accactctgc atcgctcttg aagacgtttt cccgcaatcc
    32341 gacgagccag gtgatcgtcg tggcatgggc tccgggatca ggcatcacga tgtgcccttt
    32401 ccacttggga tcggtcagat ccatgtagct cttgggagct tcggcggcgg aaaccagatc
    32461 cttgttgtag atgagtccga catattcgat tccaaaaaga tggattccat ctttcttcgc
    32521 ccaatcaggg tacccttcca tcgctttcgc ttcgtagttt gaaagcacgc cgtattcacg
    32581 caggatttcg agaaccggaa gcggtccctg cacaacgtcc gctaccagct tttttgcctc
    32641 gacttcggtc aggatcgtcg aaacataacg ggcggtggaa agtcgggtat actccacttc
    32701 cactccgctt tccgccttgt agcgctcaag aatcggttca attgccgtga tgttcgcata
    32761 catcgaaact ttcgaaggac cggtttcaac ggctttatcc gcttgtcccg cgctgaacaa
    32821 catcgttgcg gatgcaagaa gcacgccaag aatcagtaat acttttctca tattcatata
    32881 tcctccatta gtatgtaaaa aaagcgtacc atgcccccat acttcggtca aatgaaaaga
    32941 agtataccct gaaggaaaag aattataccc tgaaagaagg agctccggaa ggaattcagg
    33001 cgaacaggac cgattgcctc cggtcggtta ggaataccta aacgtcgaaa ggtacagtct
    33061 catggtacga acccaagctt ttgagttcga catcaccgat ctgtatccga ttctcgacaa
    33121 atcgggattt cctcatatca tcaggctctt gtaatattga tcgctttccg ttgggcctcg
    33181 agggccagtt cgatcgccct gaaaatgtgt tcctgagaga tggcggtctc cgtgcgattc
    33241 aaacaatcca gaatcatcct cccgaagtat gggaatccga ccttccccgc cacttcaaaa
    33301 tgcctttctt ctttttcatc gacaagaatc acatgtccgg gagtgtttga tcgggcgaca
    33361 tcagtatatt ttcgcaactc gatataccct ttcgtcccga tgacaaggag ccgaccgtca
    33421 ccccaggtgc ccaatccatc gggagtgaac caatccacat tgacatagca aggcaccccg
    33481 ttgtccgtga tgaaggtggc gtccccgaag tcttccaacc cgggatgttc cggatgattc
    33541 aggtttccca cccggcttga aaggcatttc gcatcccgag cccctgtata gaagagcatc
    33601 tgctcaagct gatgcgatcc gatgtcggta agaatgcctc cgtacttgtc cttttcaaaa
    33661 aaccaggcgg gacgggagaa gagagacagt ctatgaggtc cccatcccct gatctcgaca
    33721 acccgtccaa tggccccgtc attaatcagc tgttcggcaa acaccgcagc ctctacatgc
    33781 aatcgttcgc tgtaatacac aaaccatcgc agccccgttt ctttcacctt gcgccgtgct
    33841 tccgttacct gttcctgtga agtaaaaggc ggtttgtcgg aaaagaaatc cttgcccgca
    33901 tccagaactt ccatccctat gccgaaccgg gaagagggaa tcgctgcgct actgaccaat
    33961 ttgatctcag gatcggcgag gatctcatcc ttgctgtcgg cggcgacagc ctccgggaaa
    34021 gccttcagga aagccgccac cttttcggga tcgggatcat acaccttgga gatcgttgcc
    34081 ccggcatcct gcaatccttt gttcattcca aaaatatgac cgtggtcaag cccgatcgct
    34141 cccaccttga aagtacctct cggcacgacc ggttgagaac ttccttccgg aacatacatc
    34201 gctccgttgc tccgattatc catattcaat tcctccccaa ggtgatttca cctgacaagt
    34261 tggctaccga cttggttttt ttgaaaaact tcggaacgac ccgaagtatt ccctctttcg
    34321 tatagaaagc ggaattccgg tcaagaggaa gctccacctc acgcttctcc gcagcgcttt
    34381 gatagatggc atagatcaac tcaagagcat tcctgccgtc atacccgctt acctcaaggg
    34441 cttcttcctt tgaaatcgct ttcaacacgt tgagaatttg accctcatgc ccttcttgca
    34501 tcagatcggg aatcgcatcg aatcatcgtg acggtaccgg tcgggatggc atctcgtcct
    34561 gaaccatggc aaaggaccgt tccaagccgc tgtccaagac cgctgtgctg aattcatatt
    34621 cgaaccgtat gctcgtctca ctcagcaagc ggatatggat gatccccccg actccgcgga
    34681 aagaataacg gttgttctca tacgtgcagg attcaatgtc cttgatcgat accttcatag
    34741 tactctcttt tgatgcaaaa taatcaaatc gttttgcgta aaaatatcaa attattttgc
    34801 gtcagaaaat accatgcctt ggaggagagg tcggctgaat gaaaaagtcc cggaaacaat
    34861 caaaaaaacg gctcctaaa
//
LOCUS       DAOSLI010000016        33954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1474_length_33954_cov_25.907814, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000016 DAOSLI010000000
VERSION     DAOSLI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 33954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33954
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_33954_cov_25.907814"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLF67_03805"
     CDS             <1..667
                     /locus_tag="PLF67_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89174.1"
                     /translation="ETKITANYVVDQSTLDDLGKIDPMNGPKIFLGYAITDSAATFAG
                     LSSSQLQTAFEVRAGKAPYGRHIRSGAYNAGEGIFSIKSTFDEHLHFVVYPFSDTSGG
                     TFTSPSYHVMYSDPTNTPITLKAQIETDFTLEYKNGFFSITTTKDDLPNALYVNKTNN
                     KLVRVQNKLFSSIGLEDGDVSTYDPSNLYVHLFAAVNISQGDFNNIYWTNLKYLGNIK
                     ITE"
     gene            complement(750..1106)
                     /locus_tag="PLF67_03810"
     CDS             complement(750..1106)
                     /locus_tag="PLF67_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE89175.1"
                     /translation="MDTPMYMAIIEEVRFQIANEILKEGEQLPSIRNLAKTLKVSVIT
                     VKRAYKELEEAGYITTIPAKGSKVSLGAKERIKEEFLTTIKEHVTIAYRLAQKLKISD
                     AEFGMLHELVKAEHNG"
     gene            1155..1958
                     /gene="mazG"
                     /locus_tag="PLF67_03815"
     CDS             1155..1958
                     /gene="mazG"
                     /locus_tag="PLF67_03815"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOE89176.1"
                     /translation="MIDYTFTRSESYEEALNQLRTIIAHLRSENGCPWDRKQSGEDII
                     LNLIDEAYEYLDALRSESAPDMMEELGDVFLNVLMLGHIQEETDDTFVIGALNAVSEK
                     LIRRHPHVFSSASAENPDDVLILWDQMKVKEGKQPDEDDFFSRIPPALPIFEKAAEIQ
                     HRMRKVGFDWPDASGVFEKVREEIREVRESLEEGGDTEEELGDLLFSVINLTRFLGYN
                     PSYALRRTNKKVQARFNRVVAQAREEGLELKTASLDQLEALWQRAKGEA"
     gene            complement(1942..4335)
                     /locus_tag="PLF67_03820"
     CDS             complement(1942..4335)
                     /locus_tag="PLF67_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOE89177.1"
                     /translation="MISNPARYIGGEYIQNRKEPKEGDLRCAVCFPDLYEIGMSNLAI
                     KILYDQLNRIEDVHCDRVFSVAPDFEALLREKNLPLYTLQTRLGLHELDFLGISIGYE
                     LTATNILQVLDLGRIPIREGERGDEHPIVIGGGPAITNPLPFGAFLDFVYIGESEGGL
                     EAVVAVLRQAKREGWSRTEKIRELRQFTFLWHLGKKSTVRHIDHVFPEKRTFQHFVVP
                     NIQVAQDNGVVEIMRGCPNGCRFCHAGQFYKPYRQKELATVVEEVRQFVDEYGYREIT
                     LSSLSSGDHPQLEAMIDLLNATYAHRNISFSLPSLKVNTFSLGILEKLGEVRKSGLTF
                     AIETPKEAWQQAMNKPVPLRQVIEIIQEAKSRGWRLAKFYFMVGLPFVDREEENREIV
                     DYLGAIWDETKLNMNINIGTFIPKAHTPFQWCAQLPAAEAQDQLHHLKKMIIARIKGA
                     RVNYHDPTISYIEALVSRGDERFADVIESAYDKGCRLDAWDEYLDKEKWFKAIGEASY
                     DVNAAIYDEYRLDEPLPWDGINLRVGKPFLIEEYKRASRLLLTGRCTAECAHRCAACT
                     SAHKVVDAKRSDAVEYPKPQERPSPLSYVQALVTYRRVFPATMISHINTMRNVEMALQ
                     RANLQVQFTQGYNPTPKLEFVNPLSLGITGEAEILLIELVDDPTLTEAGVKEALQEKL
                     HDGFMITDVVFLPKGKKQTLARHLVASRYEFDTKGSIEAQSALRALVREPKEGATVKE
                     IREDRFEVIIHGERNMIKLLFGKDADKFLIASKYGITRTHLYAGEIGTSYSEFMLRL"
     gene            complement(4367..5119)
                     /locus_tag="PLF67_03825"
     CDS             complement(4367..5119)
                     /locus_tag="PLF67_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89178.1"
                     /translation="MRKTVLVGILLSFFCILVSCSNNHHFLDRLLEGGAYQEVIDRTT
                     SQFQRNKDPELLIYRARALDRLGQSSKALDVIKLYAALTPLSKQEHQELSVELALKNQ
                     DWAYLVSQAEILKERNRLTIDYAKEYYRALLKTGRTQEAKTLFSEVIRGTLSPFEEAK
                     LLIASEVDPAALETYLGMLSIEEQVNLVLEVVPLGLDPSIAEAWFISLKMQKSDTIEL
                     YRALALLAGRAGRRYEEAQYARLYQKNKEAHE"
     gene            complement(5146..5482)
                     /gene="rnpB"
                     /locus_tag="PLF67_03830"
     ncRNA           complement(5146..5482)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLF67_03830"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5492..6301)
                     /locus_tag="PLF67_03835"
     CDS             complement(5492..6301)
                     /locus_tag="PLF67_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOE89179.1"
                     /translation="MNEAAVFSRLNQLQNDLSIRFALEEEIARLPRDLKVKQELLDTI
                     NLDYIEEHSRSETAKVELKNLGYQYEDAVQARDKSERNMELITTQREFEALEKEIKDA
                     AAKEQSLLKQLHVKEKQVQEFEIRLTEKEELMALQKSEVDAEASKIDALLAEKKQELA
                     EVSARCESYIDEEITHDLYKKFSNIVKNKKGKGIVPVHGLVCQGCHVILPVQFVNSVR
                     DGKDVEFCPYCSRILYWEDVDGAEDKFIAGRVDPDADLGDFVQEGEFDDVL"
     gene            complement(6318..8267)
                     /gene="rpoD"
                     /locus_tag="PLF67_03840"
     CDS             complement(6318..8267)
                     /gene="rpoD"
                     /locus_tag="PLF67_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOE89180.1"
                     /translation="MKKLENLGDEYRVEIEELLTDNDIELVEPLETNEINGFDGEPDD
                     DEIDEVGDVFSLDLEDDDIDDEIDVDVEPISVEDFHVPEEEDDEPPKKTKDDDDDDDL
                     DEEDEDDEVDGLKSWSGISTDDDSAGGAHYVDISKLDDQDGESDSSRHPTILGTDKGE
                     GGGDDPIRLYLREIGRENLLTAEEEVELSMEMEKGAAIIIEVIQESGILITRFKEILT
                     VLSTKRDENDDEYSQKDSKEFEISLKRYNNCYRESLKDIQAVLKTYIAKKDQMILSGE
                     DILGDAALDKSRQALLKKLSTIELQPEEILAFTNDFIEAEYKIRSFNEKKQQLENKLN
                     IRTARDLRQLGKDLATQSKAVLIEEELHLSSDTIKDLIRELQITHKVLKQIEFEFEEK
                     SEVILELADRIKFGQKKMKDAKDRLIRANLRLVVSIAKKYTNRGLHFFDLVQEGNIGL
                     IKAVEKFEYRKGFKFSTYATWWIRQAITRSISDQARTIRVPVHMIEQINKVVRESRML
                     MQSLGREPTDEEVAEKLGWTEQRVKAVKNVAREPISLETPVGEEEDSLLSDFIEDKDV
                     ENPAQQTAYSLLQEQLKEVLSTLPAREQEVLKMRFGLEDGYSLTLEEVGLYFEVTRER
                     IRQIEAKALRRLRHPKRSRKLKDFI"
     gene            complement(8590..10413)
                     /gene="dnaG"
                     /locus_tag="PLF67_03845"
     CDS             complement(8590..10413)
                     /gene="dnaG"
                     /locus_tag="PLF67_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOE89181.1"
                     /translation="MAKIAESTLQQIKARLSISEVVSDYVTLSSRGGRLWGLCPFHEE
                     RTPSFSVVDQEGFYYCFSCKKGGSIFDFIMETEKVPFHEAVKMLAQRAGVELEEETPA
                     QRAQRDLSENLYELYGRIAGSFHYLLKTGSSGSYARSYLKKRNVAQPMWDTFLLGYAP
                     SDGAWLHQFLKKQHYSDELLAQSGLFSQKNPTYPLFVDRLIFPIRNWQGKVVAFGGRD
                     LSNTSKAKYINTPETVIYSKKHNLFGLYESLDTIKQERKAILCEGNFDVVALHQAGFT
                     NAMAPLGTSFTPEQGRLLRRYCDTVEILFDNDRAGREATAKALLIAQDLGMVNKVLAL
                     TNANDPSELIEQGSEEALRQQLSSSLDGFRFLLNAQLHSHDTSTAKGKARAFHQLKPY
                     LRATHSEIERQELIKTLSDLLDIDEASMHEEYAKEQTPKNVVPKREAQPVVPLNPAAI
                     SLDYYLMLTVANNPELYLKTRASIGFESLNDPKAHELYEALEQAAREDALAGSEYLLQ
                     LLESDQVKSDLSSSFGLAEFRNEPQKILKESLLRIRLRSWEKKRQSNKRLLDITQLEG
                     NDSRTIEELLEERVEIEEEIARIKQELKEETGRMASALLLL"
     gene            complement(10422..10862)
                     /locus_tag="PLF67_03850"
     CDS             complement(10422..10862)
                     /locus_tag="PLF67_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOE89182.1"
                     /translation="MSRFYDEGLQFSCVAGCVYCCGVEPGYVFLSQSDLDRLCAHTRM
                     DREAFIKTYCHYVDMGAYKMISLVEKKNHDCIFLSPNGCTVYEARPVQCRTYPFWGTI
                     IESRETWEEEARSCPGIGHGKRYSKKEIDGFIRQRIANGLIMII"
     gene            complement(10922..11004)
                     /locus_tag="PLF67_03855"
     tRNA            complement(10922..11004)
                     /locus_tag="PLF67_03855"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10968..10970),aa:Tyr,seq:gta)
     gene            11126..11197
                     /locus_tag="PLF67_03860"
     tRNA            11126..11197
                     /locus_tag="PLF67_03860"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11158..11160,aa:Thr,seq:tgt)
     gene            complement(11179..12507)
                     /gene="aroA"
                     /locus_tag="PLF67_03865"
     CDS             complement(11179..12507)
                     /gene="aroA"
                     /locus_tag="PLF67_03865"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOE89183.1"
                     /translation="MTVTIQRGALNGTMAVPGSKSDTIRAVLLALLSEGRSVIRNPLA
                     SGDGLSAKRAAIALGASITEEGNCWIVEGVGRNLRAPQTSLDLGNSGTATSFLSSIVT
                     LAGKPVTLTGDEQIRRRPFKTLFDALGDLGAGVSVHNPNQVSPPVTISGPLHGGPVTL
                     QGFNSQFVSSLLLASPFAETPVTVTVRNGLEKPYVRLTLDWMKRFGIEVANPSDYEYF
                     EIPNTKTYRAGEVAVGGDWSAVAFPLVASALTPSELTITGLNFHDSQGDKRVVDILKA
                     MGADIVEDHREGTLSIRGGKPLSALQEIDLADIPDALPALVVAATQAHGTTTFTNISH
                     VRVKETDRVFEMSERLNRLGCKLEAGENELSVHGPTPIGGGTVSSAGDHRIAMAMVAA
                     GLAAREAITITETECVAVSFPNFFERFEECGARIKKRHTEVHRREADCQN"
     gene            complement(12504..12878)
                     /gene="aroH"
                     /locus_tag="PLF67_03870"
     CDS             complement(12504..12878)
                     /gene="aroH"
                     /locus_tag="PLF67_03870"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOE89184.1"
                     /translation="MDTPRISAVRGAIQVASDSKEAIAQAAQKLFVRVLETNDLHEEQ
                     IASLLITQTGDLKSLNPATGLRMGGLASNVPLFCMPELEIEGMMKRVIRMLFILKIHK
                     NDLQPIYLDGAELLRPDLMRNE"
     gene            complement(12880..13230)
                     /locus_tag="PLF67_03875"
     CDS             complement(12880..13230)
                     /locus_tag="PLF67_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89185.1"
                     /translation="MLHERRLLAFSSRVHTYTLFLYLFFFLLYVLGGFFLVDTSFETL
                     LSFLLKLVGYSSIIFGVWVLAFSLVVWFFDKVFPFSPIFFTILRIGAVILMATIIEII
                     NTLVHRGLSIQWGG"
     gene            complement(13247..13873)
                     /locus_tag="PLF67_03880"
     CDS             complement(13247..13873)
                     /locus_tag="PLF67_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphokinase"
                     /protein_id="HOE89186.1"
                     /translation="MSHVLIITGGGAPKALEALHLEAYRFIVVADSGIEFAVRHDLFA
                     DIWVGDFDSAKIKPERVGEIIHKDHDMKLESDTELALMIGVERGMTTYTLIGGGGYRM
                     DHLLQTYSLFGRYGLPLAWHTKYESLYSVHRERRFITSAGRTLSFLPASFTGRSIVNA
                     SELVWPLESYQLTTKSISLSNRTTADLLNVTVEGDPIFLCFPVADIGP"
     gene            13940..15259
                     /locus_tag="PLF67_03885"
     CDS             13940..15259
                     /locus_tag="PLF67_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOE89187.1"
                     /translation="MPTTLKRLATYGVQPPTILIPKHEIDLKKWAVVACDQYTSEPEY
                     WKRVEAYVGDAPSTLKLIYPEVYLEEKNSQERINTIHQAMNRYLEADLFDIYEESFFL
                     IHREDEGRSSGRWGLLAALDLEHYDWKSGSHTLIRASEETILDRIPPRKLIRTGAVLE
                     LPHILVLIDDPDETIIEPLSTNRSSFHQVYSTSLMEGGGTVTAWRINDEQEFSRLADR
                     FQALLERQYTNNPLLFAMGDGNHSLATAKSCWLDIRKGLSEEEISNHPARWALVEVQN
                     IYDPGIVFEPIHRLLFSIEHQSFLDALGSFCTSYETKSLLSIEELDRCLALDDGVQRF
                     GYKDEERFLLFSLKDPASSLAAGTIQHVIDRLLDQQSATVDYTHGEDVTLRLGSLPGN
                     AAVILPNFPKSAFFKTVKEEGRLPRKTFSMGHAHQKRFYLEARKITL"
     gene            complement(15249..15683)
                     /locus_tag="PLF67_03890"
     CDS             complement(15249..15683)
                     /locus_tag="PLF67_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOE89188.1"
                     /translation="MLKRSLLAGLLTYFFTFLLLLAVFGQGGYLHNRALEAEIQRLSY
                     NQNLLQIQIQALERQEAQMGKEDALRDAAFKYGYQSEGEVVYYFPLEERESPDILEDR
                     PLEGRREYFEGVGRSVLALWALLPAFPAGMLAYVIGRRRFRG"
     gene            15761..16753
                     /locus_tag="PLF67_03895"
     CDS             15761..16753
                     /locus_tag="PLF67_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89189.1"
                     /translation="MSEKKRYGRQIFISFIVTIVILLGGVYLATQYLLPHVKWDQDQL
                     FSFFVKLFPLLIGLIMIEIGVVISKKRDEDFADDIDKLPPNAYDRPFYALPQDDPAFI
                     RNDEVIYAQAPVLEEATFSEAVLSEEPIVERAAEVILPLDLPQVEEEPEVLVVEPVAE
                     TVATELEEPILETVAAEEKEPVVGEAAPVVPPYQAPRDEISIAIEEGILQVYEMDFDS
                     ILELELESANDLNYDLTVVIVRVTEGPVDAIAQKLIRQSGDLSYTFTLEDNTIGIILP
                     FYNGEEARTFTLSLIESCQQEFQGSELEIGFASRGGRVVEKDEITQEAYKAASF"
     gene            complement(16750..17673)
                     /locus_tag="PLF67_03900"
     CDS             complement(16750..17673)
                     /locus_tag="PLF67_03900"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOE89190.1"
                     /translation="MNFEVFVLGTSGMMPLPNRNLTSAMIRREGELFLFDCGEGTQVS
                     LKKLNLKWKKINSIFISHMHADHVTGLPGILMLSSQVDRDTPLTLYGPGRLKEYVDAN
                     RRILDIYINYEIIVKTVEEGVILEEEEFVVRAFELNHTKPCFGYVFEEKKRPGLFHPE
                     VAQSLGIPMGPMWGILQKGGSVTLEDGRVIQPSDVMGDLREGRKFSYVTDSLYSPRIA
                     EYVKESDLFLCEGMFTDDLVESAREKKHMTASDAARIAKEANVKQLGLLHYSPRYSDY
                     ELRYLLKDAKKVFANTVLSKDRMRFSLPLKD"
     gene            complement(17701..18858)
                     /locus_tag="PLF67_03905"
     CDS             complement(17701..18858)
                     /locus_tag="PLF67_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE89191.1"
                     /translation="MSKEVAALLIRRSLGMVGNRASRRRILFQILLITLSLSALVFAL
                     IFVTSMSDAIANKYALINSGHLIVHDRSFPTSGSYDSYLVGTTYGLLYSKETTALVFL
                     KGVEDDYLTGKRKAELVTLKESSESTTLMKVMISKSLAQTLNVHPGENLALMIGTEAN
                     VRPTLVRIGAIYDSGYKELDELLVFTDLSSLVRLCGESVTFHHEVLVEMDRIGEVRDR
                     LIHEGYQVTTWSDEAPQIASNLITSKEVTFIIISIIGLLLGYATSELARQMVEDDKRQ
                     ITLLLLLGARRRMLGCSYLLSVLIVTIIALIFGVLGGILLSYAVPKVLPLIEQQSFSL
                     FSFYLLRFSIAVPIKRILLLGALVILSSTATVFFSLHRALRKQTIGSLAFD"
     gene            complement(18855..19553)
                     /locus_tag="PLF67_03910"
     CDS             complement(18855..19553)
                     /locus_tag="PLF67_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89192.1"
                     /translation="MANSFECVHLSSITKSFPATQAGDSRLMILEGISLVIKHGQSVA
                     IVGKSGSGKSTLLEIAAGLLEADSGTVMLDGVLLHDLDDRKKAIFRAQKMGFIFQASY
                     MLADFTALENVKIAALIAGSSPREAHAAATDLLHRVGLEKRLHHTSDKLSGGERQRVV
                     IARSLVNNPAIIFADEPTGNLDEENAAMIEELLLGLVKERNATLLLVTHNPQFASKLD
                     RVLTLRERTLVEGM"
     gene            complement(19537..20676)
                     /locus_tag="PLF67_03915"
     CDS             complement(19537..20676)
                     /locus_tag="PLF67_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE89193.1"
                     /translation="MILRLAWRYAFSKANRHRGVSIRIAVGLGIGILAILVTVSFMQA
                     LQNAQFDHLRTYESFDFHSVQRYSLEDGRALAKELEGLSGVKEAFVYQDLPVLVVDRT
                     GENHPMRIRAIDVGATLPIHPIAGELFTSEGVALSYRNRFLGNPVTITFLRSGKQVTQ
                     VPTQRSIPVTGIYATEYGPFDSATILVTPEYLASLIGSEAYSIGIHASDRSIRNVEKQ
                     LSPYDVISYKEANQSLYQAMELEQKMMTLLLFLLILVIIVHVHVSSKRLVGSKQREIA
                     LLRTLGFQEKKMPHIFVLQGLIVALIGCVLGLALTGIFFLLYPMLRVYILSNLGLDLT
                     LALRSGEVGFLCVLILALAAFGAYTGSRRLLHVDIMEILGHGELL"
     gene            complement(20686..21642)
                     /locus_tag="PLF67_03920"
     CDS             complement(20686..21642)
                     /locus_tag="PLF67_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89194.1"
                     /translation="MKQAVVIGVLLIMLSPLAGASMKANSIGQPLGGYEEGDRYRLEL
                     SSYSRVLYDEGKQVWVEIDQYIEPATVITTRTYRDGRIQKRTFTDGSLVKEQEGSTTS
                     TYTLDDAGRLIIASTAVREDEPTLAYYSYDRGKPNLISIARLGEEGLVLSRFVREPDR
                     YTVTVVDEEGGQRYVSQGSLRMSDAWKEEEELSPVVIDETEEGYLRLVQDDRTQLYSR
                     SGLVQKEYTDEYERTFYYTLEGRLKEVHTEYPSERMMVDIYDDDRTLVLRHELQNGVP
                     TKDTRYHEDFGYTETLYDREGTPYVDVTWAAGGMRVTSIRYY"
     gene            complement(21639..22511)
                     /gene="ftsY"
                     /locus_tag="PLF67_03925"
     CDS             complement(21639..22511)
                     /gene="ftsY"
                     /locus_tag="PLF67_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOE89195.1"
                     /translation="MAKGFGARLKSLFGIKRFDESYFEELEELLIEGDLGSKLTMQIS
                     DEVRNLVRKKKDLTTRELQLLVKEQLRPMVHTFHPVIDRNALNVFLILGVNGVGKTTT
                     IAKIARLYKTQGYSVTLAAADTFRAAAIDQLEVHANRVGVRIVKQESGSDPGAVVFDA
                     ITSARARGDQLLLVDTAGRMHNKEHLVRELAKIDKIVQTKGIDSVHYKKFLVIDSTTG
                     QNGLSQATIFHQAIRLDALILSKYDSLAKGGALIQIGDSLGLPISFVGVGEKYGDLEL
                     FEAEPFLDSLVGLA"
     gene            complement(22523..23755)
                     /locus_tag="PLF67_03930"
     CDS             complement(22523..23755)
                     /locus_tag="PLF67_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89196.1"
                     /translation="MKGKGSMILLLLLLAGGGLLFAQTPAILIIAGSEAAEPIVFELQ
                     SLLAEGVPRVVLTDDRKAILKEYDQEEIDRAFLAEQAQAYAKKQPAKPKGEAFEDTGS
                     DLPSTYASKGYDPSLLALFEREEALFWYMSRERALGVFLVDVTPYGQARRVRLLYQEG
                     PRLDLRLIHDRLLVTMEERTLIEELLAQAVHILTDGTKAVLKIEGSKEIRIAEKMERI
                     ESLDVYLMPTDKSTVTVERSGHEPRKVAVRWEEGEVATIRPDLQRIVNEPVTLLSNVG
                     AVRYSSEGEAGESGMITLGSPSYPIALSVEKEGFVKSVLHIPHETGTVIRFGVRKASV
                     DVPDEQKRVYRRALGTILSFGSYVAAQALVAAYDPKHENPLWSLALGSTRLLSVVSSV
                     AMVAELSSYASAVGMMQE"
     gene            complement(23759..24860)
                     /gene="prfB"
                     /locus_tag="PLF67_03935"
     CDS             complement(join(23759..24799,24801..24860))
                     /gene="prfB"
                     /locus_tag="PLF67_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_144082914.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOE89197.1"
                     /translation="MFFQNKALLEEVKEEAYTVWGVFESSSIRKEIQTLEERTLQPTF
                     WDDRKRAEETFSRLNSLKDSYEPWKELIEEIDDLTELVELYADEPDNAEEQEQVDAHI
                     AAVADTYQKLKLKTLLDGEFDKNDTFLTIHAGAGGNEASDWAQMLMRMYLRYSELMDF
                     KTQILDLQEDEGGIKSASIKISGPYSFGYLKGEAGVHRLVRISPFDSQKRRHTSFTSV
                     YASPVIDDEIEIDLKPEDYRLDTYRAGGAGGQHVNKTDSAVRITHFESGIVVQCQNER
                     SQIMNKEVAFKMLRSRLYEYYRAIRDEERAKNAIEKKDIAWGSQIRSYVFQPYTMVKD
                     HRTNESVGNIQAVMDGQIHPFIEAYLQWQHSE"
     gene            complement(24879..26774)
                     /locus_tag="PLF67_03940"
     CDS             complement(24879..26774)
                     /locus_tag="PLF67_03940"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HOE89198.1"
                     /translation="MKRLIGGYTQLAHGLPAPLFTRALEAVLLPLLTELNRSENVVFQ
                     LALSVPFMEFLEKNHPSVNLLLADLCRNEKLEFLSHAYFQSIPSLLAPKDRAYQIEQT
                     TTYMRKRYQTRSRTLFCPHQIFSPAYIGSMNLCFLDTLMVSAHDPCIEKTYFDEPVIM
                     QELGKRGIILPIDDVVSRSVEAFSRKAIPFSKLHSAVRQVVESDRRFVLALLNFDQLS
                     TGGITPEETGQLFSALVQKGSSSTEEIEGQELPLKRGYLPMGWYGHDIKRKGLCCFNE
                     LLVQDETLYYLQGRYMTTSEYARSYKKDRDVKRRLEQLISRASVGSIYIGDAYDTFLY
                     SQVRKLYWRSIHEIDSILASLKDFSYPNVSDYDHDGLDEHLVISKFLSCVIDSKGGAL
                     SELTYLPSLHNYGDSFNPLPIYGTTDHLLHPPKPGVKQRIFTDLFFDENYVVDEYDKR
                     DKENVLNLEERVYGLEVYDRRSTEYVLRTVCDQGPFEGGELGVIKHYKIRQNTVLLDI
                     TLTNNGEEELLFRYGTELVLALSPPKEAVSVILMDNRKSVMKEEKEFSSAQVKSLKIY
                     DEPNHTQLTLVSDTRFSLVKEDCTIAEAHGEQRHYQHTTLLCAYEIALAPARERKITL
                     GLRIERK"
     gene            complement(26777..27577)
                     /locus_tag="PLF67_03945"
     CDS             complement(26777..27577)
                     /locus_tag="PLF67_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOE89199.1"
                     /translation="MNILLAIIIVAVLGGIFGYGLSYAEKKLAIEKDPKVLALEPIMP
                     GANCGVCGYAGCNAYVAAVVKGEAELGLCTPGGPSLVEKMSEIMGLKVDSVDEVRRMV
                     AHVMCRGNPEVTKRDYRYEGLEDCNAATLLLGGDTTCKSACLRLGSCINVCPTGAISK
                     DEKGYIIVDEKKCISCEKCVLVCPTGAMQMIGADDEYVVECNNHEPGGRVRKYCTVGC
                     IGCKICETKYPESGCTVDRFLATFNQLAEHNQIADAAEACPTKCIVKL"
     gene            complement(27587..28180)
                     /gene="rsxA"
                     /locus_tag="PLF67_03950"
     CDS             complement(27587..28180)
                     /gene="rsxA"
                     /locus_tag="PLF67_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOE89200.1"
                     /translation="MSYIAILITFIFINNFILVQFLGLCPFIGVSKNSENAIGMGASV
                     TFVTTVASVVTWAIYHFLLVPFNLAYLQTLTFILVIATLVQLLEMVIQKVSPPLYKAL
                     GIYLPLITTNCAVLGIAIINITNNYNVMESFTAGLAAGLGFTLAIVLMSNVRERLDLH
                     PVRKAYRGMPIAFISAGLMALAFMAFDKSLLTNLHLV"
     gene            complement(28182..28802)
                     /gene="rsxE"
                     /locus_tag="PLF67_03955"
     CDS             complement(28182..28802)
                     /gene="rsxE"
                     /locus_tag="PLF67_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOE89201.1"
                     /translation="MNIKELTKGIFKENPVFIIVLGLCPVLGVSTQVSNAIGMSAGVL
                     FVLLFSNIIISALRNIIPDSIHIPAYIVIIASLVTIIEMLMHAFAPAVYAALGVYLPL
                     IVVNCIILGRAEAFASNNTVLDSALDAIGMSIGFALSLTLIALIREVLGAGTITLFPV
                     GSFSGVIEVPWLVDNPFKVMALAPGALLLMGFLKAFFDWKAMRKAG"
     gene            complement(28802..29356)
                     /locus_tag="PLF67_03960"
     CDS             complement(28802..29356)
                     /locus_tag="PLF67_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOE89202.1"
                     /translation="MKKNLKYSLILLSICAISTALLALTNAITAPVIERQGQASRLAG
                     LEAVSMGYEAGAQKVVTDDPTVTFMIELKDDRRTAGYILGLKGAGYGGEMTLVASYKT
                     DGEILRVQLLSHNETPGLGKKAENEGYMDKFEGTGAAQAVPTNKSMLNSEDAAAVSGS
                     TVTFVAIGKALEAGSRYVIRLGGK"
     gene            complement(29353..30444)
                     /locus_tag="PLF67_03965"
     CDS             complement(29353..30444)
                     /locus_tag="PLF67_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOE89203.1"
                     /translation="MTEQPLMTVSSSPHLHDGSSTANIMWHVSLSLVPAALWGIYVFG
                     LYSLVIILVSIVSAALTEFLLGKIGKECTIWDGSAVLTGFLVGLNMPPSIPLFIPIVA
                     SFFAIFVAKWTFGGLGANWANPALSGRVFVFFSFTTQMSTFTTPRTLLEAMPDTLSGA
                     TPLGFMKTAVSGGSCGATTNELLSWVGYPATPIAQKIGAVLGINAYTIDTFLGNVAGS
                     IGEVSALLLLIGGIYLLIRRIITWHIPVTYLLSSALLSRVFGGLPLGLGIFQGTFFPS
                     MFSGGLLFGALFMATDYVTSPVSSKGKIIFGIGCGFFTFLFRSFGSMPEAVSVSILLM
                     NIITPTIDRYIRPRKFGEKKLFARKEAVA"
     gene            complement(30444..31754)
                     /gene="rsxC"
                     /locus_tag="PLF67_03970"
     CDS             complement(30444..31754)
                     /gene="rsxC"
                     /locus_tag="PLF67_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOE89204.1"
                     /translation="MHAVPTFRKGGVHPPDQKVFSREQEIVRLPLPSELVVALSQHLG
                     APAKPLKAKGDAVERGEKIGESVGFISADVHSPVNGTIKEIKTVTLANSVQCDAFVIA
                     VDEKQPKDTPKPVDWKKLKKEELLASVKEHGIVGLGGATFPTHVKYAVPRDKNVEYFL
                     VNGADCEPYLTCDYRLMLEKGKEALEGALIAAKILEAKKIIVGVELNKTEAIEHLKQI
                     IREEAWPIEVVGLKVKYPQGDEKQLIKATINREIPSGKLPIDVGAVVSNIGTCYAIYE
                     AIVYKKPLYERIISVTGEGIVNPKNVLAPIGTKVSDLLEFCGGLQGEVEKLIIGGPMM
                     GFAFFDTETPVTKGSDGILALAAKKKARQTPCISCGKCVDACPIGLVPTLLYKQITNH
                     NYEVAMNSLSLMDCKECGCCSYVCPAHLPLVHTFKTGKKMGRKK"
     gene            31849..32721
                     /locus_tag="PLF67_03975"
     CDS             31849..32721
                     /locus_tag="PLF67_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89205.1"
                     /translation="METMNRIFKYAFWKLKRTKVYALVGKSGTGKSFRSKILAERYHI
                     DYIIDDGLLIKGDRIIAGKSAKREEHILAAVRTAVFGDEDHYLEVLTALKKEKVHRIL
                     IIGTSEKMVYKIAERLELQPPERIFHIEEIATKEQIETAMRIRYAEGKHVIPVPSIEI
                     TRTYPQIVYDSMRVFRRAKQSKVFRKQNAGFEKTIVRPEFSKYSKTSVSEAALTQMVA
                     HCLDQFDSQIRVTAVHVLSESDGYALRVKLRVPSHHQITTSLRELQEYIADSLERYGG
                     IFVFSVHIEIEEWG"
     gene            complement(32718..33878)
                     /locus_tag="PLF67_03980"
     CDS             complement(32718..33878)
                     /locus_tag="PLF67_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(A) polymerase"
                     /protein_id="HOE89206.1"
                     /translation="MLIRYKQDDQGNTLPIANIYTQAEHKIETRNIDRDALWVIKKLQ
                     GSGAEAYLVGGAVRDLMLGNIPKDFDIATSASPRQVQRLFYNARIIGRRFKLVHLIFK
                     DKVLEVSTFRSGEEAEDGSNNVFGSIEQDAKRRDFSINSFYYDPSNGQLLDFNDAMED
                     MRKKRISSVIPLEESFTEDPVRMIRAVKYAVITGFSLRFKLRVALRKYAPELSRISSS
                     RLTEELVKILSSGHAAEIFADLNRYKILPFMMPAFTIYCSYPQVRESIRGLDSRVQAA
                     KTNNGEEIEFAEMLKALVDPLIVFSDASMSPTNRFKETFRQIKVLISPMTPSNYDVEL
                     AAVRILSERGFRTPRNAVRRPPQPQRRGGASPVTRPTTPKRKRPPAKQRKEA"
BASE COUNT         7714 a         9441 c         8641 g         8158 t
ORIGIN      
        1 agaaacgaag attaccgcca actatgtggt ggatcaaagc acgttggatg accttggcaa
       61 aatcgatccg atgaacggac cgaagatctt cttagggtat gcgatcaccg attcggcagc
      121 cacattcgcc gggctttcat cgtcgcagct ccaaacggcg tttgaagtga gagccggcaa
      181 agccccatat gggcggcaca tccgatccgg cgcttacaac gcaggtgaag gaatcttttc
      241 gatcaaaagc acttttgacg aacatcttca ctttgttgtc tatccgttca gcgacacttc
      301 aggcggcacc ttcacatcgc caagctatca tgtgatgtat tccgatccga ccaacacccc
      361 gatcacattg aaagcccaga ttgaaactga ttttacgctt gaatataaga acggcttctt
      421 ttccataact actaccaaag atgatctacc aaatgcttta tatgtgaata agacgaacaa
      481 taagcttgta cgagtacaaa ataaattgtt ttcgtcgatc ggacttgaag acggcgatgt
      541 gagtacatac gatccatcca acctgtacgt ccatctcttt gcggcggtca acatttccca
      601 aggcgatttc aacaatatct attggacgaa cctgaagtat ttgggaaata tcaaaattac
      661 cgagtaatat aaataatata tattctgtca actagaaata gtgaaagaat tcccctgttg
      721 gggttcgttc tgccccgtct gtttgtttct catccgttgt gttccgcttt gaccagctca
      781 tggagcatac cgaattccgc gtccgagatc ttcagttttt gcgccaagcg atatgcgatt
      841 gttacatgtt ccttgatcgt tgtaagaaat tcctctttga tccgctcctt ggcgccaagg
      901 gaaaccttcg agccttttgc aggaatcgtg gtgatgtatc ctgcctcttc caattccttg
      961 tatgcccgct ttaccgtgat cacgctgacc ttgagggttt tcgcaagatt ccggattgac
     1021 ggaagctgtt cgccttcctt gagaatttcg ttagcgatct ggaagcgaac ctcttcgatg
     1081 atcgccatat acatcggtgt gtccatagat tatacacagt gtaccatgcc gaaagatttc
     1141 agtatacttt ttttatgatt gattacacat ttacccgctc tgagagctat gaagaagcac
     1201 tgaatcagct caggacaatc atcgctcatc tgcgcagcga aaacggctgc ccatgggatc
     1261 gcaagcagtc cggcgaagat attatcctga acctgatcga tgaagcctac gagtacctgg
     1321 atgcgctgag aagcgaatct gcgcctgata tgatggaaga acttggggat gtctttttaa
     1381 acgtcctgat gcttggccac attcaggaag aaaccgatga cacgttcgtg atcggggcgt
     1441 tgaatgccgt cagtgaaaag ctcattcggc gtcaccctca tgtcttttcc tccgcttcgg
     1501 cggagaatcc tgatgatgtc ctcatacttt gggatcaaat gaaggtcaaa gaaggaaagc
     1561 agccggatga agacgacttc ttcagtcgca ttccccctgc tcttcccatc ttcgaaaaag
     1621 ccgctgaaat ccagcataga atgagaaagg tcggtttcga ttggccggat gcttccggag
     1681 tcttcgagaa ggtccgggaa gaaattcggg aagtgcgtga atcattggag gaaggcggcg
     1741 ataccgagga agaactgggg gatcttctct tcagcgtcat caatcttact cggtttctgg
     1801 gctacaaccc ctcctatgcg cttcgtcgga caaataaaaa ggtgcaggcc cggttcaacc
     1861 gggtagtcgc ccaagcacgg gaagaaggtt tggaactcaa aacggcgagt ctcgatcagt
     1921 tggaagcgtt atggcagcga gctaaaggcg aagcatgaac tcactgtagg aggttccgat
     1981 ctcccccgca tagaggtgcg ttcgggttat tccatatttg gaagcgatca ggaacttgtc
     2041 cgcatccttt ccgaagagca gcttgatcat attgcgttcg ccatggatga taacctcaaa
     2101 gcgatcttct cggatctctt tcaccgtggc gccttctttg ggctcccgaa ccagtgctcg
     2161 aagagcgctt tgagcctcaa tggatccttt tgtatcgaac tcatagcggc tggcgacgag
     2221 gtgccgggca agggtttgct tcttcccttt cgggagaaac acgacatccg tgatcataaa
     2281 accatcatgg agtttttcct gcagggcttc ttttacgccc gcttcggtga gagtcggatc
     2341 atcaaccagc tcgatcagaa ggatttccgc ctcccccgtt attccgaggg aaagcggatt
     2401 cacgaactca agcttgggcg tcgggttgta cccttgggtg aactgcacct gcaggtttgc
     2461 ccgctgaagc gccatctcga cgtttctcat cgtattgatg tggctgatca tggtcgcggg
     2521 aaagacccgt ctgtaggtaa cgagcgcctg cacataggat agcgggctcg gccgctcctg
     2581 cggcttcggg tattcgacgg catccgaccg tttggcatca accaccttat gggcggaagt
     2641 acatgccgca caccgatgag cacactcggc ggtgcaacgg ccggtaagca ggagcctgct
     2701 cgcccgttta tactcctcga tgaggaacgg tttaccgact cgaagattga ttccgtccca
     2761 gggaagcggt tcatcgagcc gatactcatc gtagatcgcg gcgtttacat cataggaagc
     2821 ctccccgatc gccttgaacc atttctcctt atcaaggtat tcatcccagg catcaagccg
     2881 gcagcctttg tcgtaggcgc tctcgatgac atcggcaaac ctctcatccc cccggctgac
     2941 cagagcctcg atgtaactga tcgtcggatc gtgatagttc acccgggccc ctttgatgcg
     3001 ggcgatgatc atcttcttga ggtggtggag ctggtcttgc gcttcggcgg ccggaagctg
     3061 ggcgcaccat tggaacggcg tatgggcctt gggaatgaag gtgccgatgt tgatgttcat
     3121 gttgagcttc gtttcatccc agatcgcccc gaggtaatcg acgatctccc ggttttcctc
     3181 ctctcgatcc acgaacggaa ggcccaccat gaaataaaac ttcgccagcc gccatccccg
     3241 gctcttcgct tcctgaatga tttcaatgac ttgccgcaag ggaaccggtt tgttcatcgc
     3301 ctgctgccac gcttctttcg gcgtctcgat agcgaaagtc aatccgcttt tccgcacctc
     3361 cccgagcttc tccaggattc cgaggctgaa ggtgttcacc ttcagggaag gaagggaaaa
     3421 ggagatgttc cgatgggcgt aggttgcatt gaggaggtcg atcatcgctt ccaattgggg
     3481 atgatcgccg ctggaaagcg aggaaagagt gatttcccgg tacccgtatt catcgacgaa
     3541 ctggcggacc tcctcgacga ccgtggcaag ttctttctgg cggtagggtt tgtagaattg
     3601 gccggcatgg cagaaccggc agccgttggg acagccgcgc atgatttcca cgacgccgtt
     3661 gtcctgcgcc acctgaatat tgggtacgac aaagtgctga aacgtccgtt tttcaggaaa
     3721 gacgtgatcg atatgccgca cggtggattt tttacccaaa tgccataaga acgtgaattg
     3781 tcgcaactcc ctgatttttt ccgtccgact ccacccttcc ctctttgctt gccgtaatac
     3841 ggcgaccacg gcctccagac cgccttccga ttcgccgata tacacgaagt caaggaatgc
     3901 tccgaacggc agcggattgg tgatcgccgg cccgcccccg atcacgatcg gatgctcgtc
     3961 cccccgctcg ccttccctga tcggtatcct gcccagatcc aagacttgaa ggatgttcgt
     4021 cgccgtcaat tcatagccga tcgagatgcc gagaaaatcg agttcatgaa gaccaagccg
     4081 ggtctggagc gtgtataacg gaagattttt ctccctgagc agcgcttcaa agtcgggagc
     4141 gacggaaaag acgcggtcgc aatggacgtc ctcgatgcga ttcagttgat cgtagaggat
     4201 cttgatcgcc agattgctca tcccgatctc atagaggtcg ggaaaacaga ccgcacatcg
     4261 aagatcgcct tctttgggct ccttcctgtt ttggatatat tcaccgccga tataccgggc
     4321 ggggttgctt atcatcaaca agtcattgtg taatcgatcg aacgtattat tcatgagctt
     4381 ccttgttttt ttggtaaagc cgggcgtact gtgcttcctc gtatcgccgc ccggctcggc
     4441 cggcaagcag agccaaggcg cgataaagct cgattgtatc actcttttgc atttttaggg
     4501 agatgaacca cgcttcggcg atcgagggat ccaatccaag gggaaccacc tccaatacga
     4561 ggttgacctg ctcttcaatt gaaagcatgc ccagataggt ttcaagggcg gcgggatcga
     4621 cttcggaggc gatgaggagc ttcgcctcct cgaacggtga aagggttccc cggatcactt
     4681 ctgaaaagag ggtctttgct tcctgcgtcc tgcctgtttt caggagcgcc cggtaatact
     4741 cttttgcgta atcaatcgtc aggcgattcc gctcttttag gatctccgcc tggctgacga
     4801 ggtacgccca gtcttggttt ttcaaagcga gctcgacact cagctcctgg tgctcctgtt
     4861 tggaaagagg cgtcaatgca gcgtagagct tgatcacatc cagagctttt gatgattggc
     4921 cgaggcggtc gagcgcccga gctcgataga tgagcagctc ggggtcttta ttccgctgaa
     4981 attgcgatgt tgtgcgatcg atcacctcct gatatgctcc accctcgagc aagcgatcaa
     5041 gaaagtgatg gttgtttgaa caggagacga ggatacaaaa aaaggagaga agtatgccaa
     5101 caagaactgt ttttcgcata tcttcacccc ttctatgtaa aaaaaaagac aaggtaggca
     5161 ataagccggg ttctgtcgaa gatgatcatc tatctaggtg gtttgttgcc aaaccactca
     5221 tgcaacctac ccgcaaatgt cgggcggaca acccaatttg ctgcttggtc ttgctcctga
     5281 cgaggttttc cctgccgatg atgttgccac catcgcggtg ggctcttacc ccaccatttc
     5341 acccttaccg cggatgcggc ggtatctttt ctgtggcact ctctgtagcg ttaccgctcc
     5401 cggatgttat ccggcgtcat gtcctgcgga gcccggactt tcctcactcg gcataccgag
     5461 cgcgatcatc tagcctacct tgtcaaaaat cctataaaac gtcgtcaaac tcaccttctt
     5521 gaacaaaatc acccaagtcg gcatcgggat cgacccgtcc ggcgatgaac ttgtcttccg
     5581 caccgtcgac gtcttcccaa tagaggattc ggctgcagta cggacagaat tcaacatcct
     5641 tgccatcgcg aaccgagttt acgaactgca ccggaagaat aacatggcat ccctgacaaa
     5701 ccaagccatg aacgggaacg attcccttgc ctttcttgtt tttcacgatg ttggaaaact
     5761 ttttgtacaa atcgtgtgtg atctcttcat cgatgtagct ttcacatctc gcgctgactt
     5821 cagccagttc ctgtttcttt tcggcaagga gcgcatcgat cttgctcgcc tcggcatcga
     5881 cttcactttt ttgaagagcc atcagctcct ctttctcggt aagtcggatt tcgaactcct
     5941 gaacctgctt ctctttgacg tgaagctgtt tcaacaggct ctgctctttc gctgcggcat
     6001 ctttgatctc tttctccaac gcttcgaact cacgctgggt ggtgatcaac tccatgttgc
     6061 gctcgctttt gtcacgggct tggacagcat cttcatactg gtagccaagg ttcttcagct
     6121 cgaccttcgc cgtctcgctt cgactgtgct cttcgatgta gtcgaggttg atcgtgtcca
     6181 acaactcctg tttgactttc aagtcacggg ggagcctggc gatctcctct tccagagcaa
     6241 atcgaatgct caaatcattc tgtaattgat taagccgtga aaataccgct gcttcattca
     6301 tcatcattct tcctctatta aatgaaatcc ttcaattttc tgcttcgttt cgggtggcgg
     6361 agcctccgca aagctttcgc ttcaatctgt cgaatccgct cgcgtgtaac ctcaaaatac
     6421 aaccctactt cctcgagtgt caacgagtag ccgtcctcta agccgaaccg catcttcaac
     6481 acttcctgct cgcgggccgg aagcgtgctg agcacctctt tcagttgctc ctgaagcaat
     6541 gaataggcgg tttgctgagc gggattctca acatccttgt cctcaatgaa atcactgagg
     6601 agggaatcct cttcctcgcc gaccggtgtt tcaagactga tcggttcgcg ggcgacgttt
     6661 ttgacggcct ttacccgctg ctcggtccaa ccaagctttt cagcgacttc ctcatcggtc
     6721 ggctcccgcc cgaggctctg catcaacatc ctcgactcgc gaacgacttt attgatctgt
     6781 tcgatcatgt ggaccgggac gcggatcgtt cgggcctggt cactgatcga acgggtgatc
     6841 gcctgacgaa tccaccacgt ggcgtaggtg gaaaatttga agcccttgcg gtactcgaac
     6901 ttctcaaccg ctttgatcaa gccgatattc ccttcctgaa caaggtcaaa gaaatgaagg
     6961 ccgcgattgg tatacttctt ggcgatcgag acgaccagcc gcaggttggc ccgaatcagg
     7021 cgatcctttg catctttcat cttcttttgg ccgaatttga ttcgatcggc caactcgaga
     7081 atgacttcac ttttttcctc aaattcaaac tcgatctgct tgagcacttt gtgagtgatc
     7141 tgaagctccc gaatcagatc cttgatcgta tcgctggaaa ggtgcagctc ttcttcgatc
     7201 aggacggctt ttgactgggt cgccaaatcc ttgccgagtt gacggagatc ccgtgctgtt
     7261 cgaatattca gcttgttttc aagctgttgc ttcttttcat tgaaggaacg aatcttatac
     7321 tccgcctcga tgaagtcgtt ggtgaacgca agaatctctt ccggctgcaa ttcgatcgtc
     7381 gagagtttct tgagcaatgc ctgtcgactc ttatccaaag cggcatcgcc gagaatatcc
     7441 tctcccgaga gaatcatctg gtctttcttt gcgatatagg tctttaaaac agcttgaatg
     7501 tccttcaacg attcccggta gcaattgtta tatcgcttca ggcttatttc aaattctttg
     7561 gaatcctttt gcgaatattc atcatcattt tcatcccgct tcgtagaaag caccgtcagg
     7621 atttccttga atcgggtaat aagaattccg ctttcctgga ttacctcgat gatgatcgcc
     7681 gccccctttt ccatctccat ggaaagttcg acctcttctt cggcggtaag aagattctcc
     7741 ctcccgatct cgcgcaaata aagtcgaatc ggatcgtccc cgccaccctc gcctttatcg
     7801 gttccaagga ttgtcggatg acgggaggag tcgctttccc catcctgatc gtcgagtttg
     7861 gaaatatcca cgtaatgggc tccaccggcc gagtcatcgt cggtgctgat gccgctccat
     7921 gatttcaacc catcgacctc gtcgtcctca tcctcttcat cgagatcgtc atcgtcgtca
     7981 tcgtctttcg tttttttggg gggttcgtca tcttcctctt caggcacatg aaaatcttct
     8041 acgctgatcg gttcaacatc gacgtcgatc tcatcgtcaa tatcgtcatc ctccaaatcc
     8101 aggctgaaga catcaccaac ctcatcgatc tcatcgtcat cgggctcccc gtcaaacccg
     8161 ttgatctcat tggtttcaag cggctcgaca agctcgatat cgttgtcggt caagagttcc
     8221 tcaatttcta ctcgatattc atcgcccaaa ttttcgagct tcttcataag gaggtctttg
     8281 ctgacgcttt tcgtgtcccc cgcttcttta actaaatcgt taaaaatgtt attgacaata
     8341 gtgttatcca gcatctgttt cctttaccct gtttctctat ccacactccg aatccgtgtt
     8401 ggagtttgtg cgcgttaaaa agccttggtt tgaatcaacc aagtgaaccc aaaggtgtat
     8461 tacgtcagta tgttctctcc cttcgtatac tcgtcatctt tcagcacttc gtcaatcttg
     8521 ggtcatgcgg gcccaagtcg gtgacctttt tcacggacgt cgttacatca tacagattta
     8581 tccgtgcctt tacaagagaa gcaaagctga tgccatcctc cctgtttcct ccttcagctc
     8641 ctgctttatt cgggcgattt cctcctcaat ttccactcgt tcttccaata gctcttcaat
     8701 tgtccggctg tcgttcccct caagctgcgt gatatccaac aagcgtttgt tcgattggcg
     8761 ctttttctcc catgatcgaa ggcggattct caagagactc tctttcagga tcttctgcgg
     8821 ttcgtttcga aactccgcca atccaaatga tgaggaaagg tcgcttttta cctggtcgct
     8881 ctcgagcaat tgcaagagat actcactgcc tgcaagggca tcctcccggg cggcctgctc
     8941 caacgcttca tacagttcgt gggctttcgg atcattcagg ctttcaaatc cgatcgaagc
     9001 gcgagttttc aagtacagct cggggttgtt cgccacggtc agcatcaggt aatagtccaa
     9061 agagatcgcc gccggattca acggaacaac cggttgtgct tcccgcttgg gcacgacgtt
     9121 cttcggggtt tgctccttgg cgtattcctc atgcatcgag gcttcatcga tatccaggag
     9181 atcgctgagg gtcttgatca attcctgccg ctcgatctcg ctgtgcgtcg ccctcagata
     9241 gggtttgagc tgatgaaatg cacgggcctt cccttttgcc gtggacgtat catggctgtg
     9301 gagctgagcg ttgagcaaaa atcggaatcc gtcgagggaa gaggagagct gttgtctgag
     9361 cgcttcttcc gagccctgct cgatgagttc cgacggatca tttgcattcg tgagggcaag
     9421 caccttgttg accatcccca gatcctgggc gatcagcaac gctttggcgg tcgcttcccg
     9481 cccggcccgg tcattgtcaa aaagaatctc gacggtatcg cagtatcggc ggagcagcct
     9541 cccctgctcc ggggtgaacg aggtgcccag gggcgccatc gcattggtga accccgcctg
     9601 atggagcgcc acaacatcga agttcccctc gcaaaggatc gctttgcgct cctgtttgat
     9661 cgtatccagc gattcataga gcccgaagag gttgtgcttc ttcgaataga tcaccgtctc
     9721 cggagtgttg atgtacttcg ctttggaggt gtttgaaaga tccctccccc cgaacgccac
     9781 gaccttgccc tgccagttgc ggatggggaa aatcaaccgg tccacgaaga gcggataggt
     9841 cggatttttt tggctgaaga ggccgctttg tgccagcaac tcgtcgctgt aatgctgttt
     9901 cttcaaaaac tgatgcagcc acgccccgtc gctcggagca tatccaagga gaaacgtgtc
     9961 ccacatcggt tgagccacgt ttcgcttctt cagataggag cgggcatatg atccgcttga
    10021 cccggttttc aaaagatagt ggaaggagcc ggctatccgc ccgtacagct catacaggtt
    10081 ttcggaaaga tcccgctgag cccgctgggc gggggtctcc tcttccagtt ccactcccgc
    10141 tcgttgggca agcatcttca ccgcttcatg gaacggcacc ttttccgtct ccatgatgaa
    10201 gtcgaagatt gatccccctt ttttacagga aaaacagtaa taaaacccct cttgatcgac
    10261 gacgctgaag gaaggggtgc gctcttcatg gaagggacac aatccccaga ggcgcccgcc
    10321 tctcgaggag agggtgacgt aatcactgac cacttcgctg atcgacaggc gagctttgat
    10381 ttgttggaga gtagattcag caatctttgc catctggtct cctatatgat catgatcaat
    10441 ccattggcga ttcgctggcg aatgaaacca tcaatttcct tcttgctgta ccgtttaccg
    10501 tgtccgattc cgggacatga acgggcttct tcttcccatg tctctctgct ttcaatgatt
    10561 gtgccccaaa acgggtaggt tcggcattgg accggacgag cttcatacac ggtgcatccg
    10621 ttcgggctca ggaagatgca gtcatggttc tttttctcca ccaggctgat catcttgtaa
    10681 gcccccatgt caacgtagtg acagtacgtc ttgatgaatg cttcccgatc catgcgggta
    10741 tgggcacaaa ggcgatcaag atcggattga gagagaaaga cataccccgg ctcaaccccg
    10801 caacaataga cgcagcctgc tacgcaggag aattgcaggc cttcatcgta aaacctactc
    10861 atctacaccc ctttttcacg atgacgaaca aaggccttcc tcgtagaaga aggcctccga
    10921 ttggagagag aaggattcga accttcgaag gtgataacca acagatttac agtctgcccc
    10981 ctttggccgc ttgggtacct ctccctgtca aaagacaaac gtacaataca agctattggg
    11041 ccctttgtca accgcacgaa gggattttgc ccaaaaaccc gcttcttgac acgtccatgc
    11101 tccgccagat atagttgcac gcgatgccga cttagctcag cggtagagca cgtgacttgt
    11161 aatctcgggg tcgtcagttc aattctgaca gtcggcttcc cggcggtgta cttcggtatg
    11221 ccgttttttt attcttgccc cgcactcttc aaagcgttca aagaagttgg gaaaggagac
    11281 tgcgacgcac tccgtttccg ttatcgtgat cgcctccctt gcagccagac cggctgcgac
    11341 cattgccatt gcgatccgat ggtccccggc ggaggaaacc gttccacccc cgatgggagt
    11401 cggaccgtgg acggagagct cattctcccc cgcctcaagc ttgcagccca atcggttgag
    11461 ccgctcggac atctcgaaga cccggtcggt ttccttgacc cggacatggg agatattggt
    11521 gaacgtcgtc gtcccatgcg cctgagtcgc cgccacgaca agggcgggaa gcgcatcggg
    11581 gatatccgca agatcgattt cctgcaaggc ggaaagcggt ttcccgccgc gaattgaaag
    11641 ggttccttcc cgatgatctt caacgatgtc ggcacccatc gctttgagaa tatcgaccac
    11701 ccgtttatcc ccctggctgt catggaagtt caggccggtg atcgtgagtt cggagggagt
    11761 caatgcggaa gcgaccagag gaaacgccac cgccgaccaa tcgcctccca cggctacttc
    11821 acctgcccgg taggtcttcg tattcggaat ctcaaagtat tcatagtcgc tcgggtttgc
    11881 cacctcgatc ccgaaccgct tcatccaatc gagcgtcaac cgaacatacg gcttttccaa
    11941 tccgttgcgg acggtgacgg tgacgggagt ttccgcaaac gggctcgcca gaagcaggct
    12001 ggagacgaat tggctgttga agccttgaag cgtgacgggt cctccatgaa gagggcctga
    12061 gatcgtgacc ggcggggaga cctgattggg attatgcacc gaaacccctg cccccagatc
    12121 cccgagggca tcgaacagcg ttttgaatgg ccggcgccgg atctgttcat cgccggtgag
    12181 cgtaaccggc tttccggcca gcgtgacgat tgaagacaag aaggaggtcg ccgttccgct
    12241 gtttcccaga tcaaggctcg tttgaggtgc tcggagatta cgccccactc cttcaacgat
    12301 ccaacaattt ccttcttccg taatggaagc tccaagagcg atcgccgccc tttttgcgga
    12361 aagtccgtct ccggaggcca gcggattgcg gatgaccgaa cgtccttcgg aaagcagcgc
    12421 aagaagaaca gccctgatcg tgtcgctttt agagccggga acggccatcg ttccattcag
    12481 ggctcccctt tgaatcgtta cggtcattcg ttcctcatca gatcgggccg caggagttcc
    12541 gccccgtcaa ggtagatcgg ctgaagatcg tttttgtgga tcttcaggat gaacagcatc
    12601 cggatcaccc gtttcatcat cccttcgatt tcaagctccg gcatgcagaa gagcggcacg
    12661 ttggaagcaa gacctcccat cctcaagccc gtcgccggat tcagggattt cagatccccg
    12721 gtctgggtga tcagcaacga tgcgatctgc tcttcgtgca ggtcgtttgt ttccaatacg
    12781 cgtacaaaga gtttttgtgc cgcttgagcg attgcttctt tggaatcgga agcgacttga
    12841 atggcgccgc gcaccgccga gattcgaggt gtatccatct tacccgcccc actggattga
    12901 aagtcctcga tggaccaacg tattgatgat ttcgatgatt gtcgccatga gaatcaccgc
    12961 accgattctc agaatcgtga aaaagatcgg tgaaaacgga aataccttat caaaaaacca
    13021 gacgacgagg gagaacgcca gtacccatac tccgaagatg atgctggagt atccgaccag
    13081 cttcagcaaa aagctcagca acgtttcaaa ggatgtgtcg acgaggaaaa acccgcccag
    13141 cacatagaga aggaagaaga acaggtacaa aaagagagtg taggtgtgga cccgactgga
    13201 aaacgccagc agtcttcgtt cgtgcagcat gcttcgatcg tagcacctaa ggcccgatat
    13261 ctgcaaccgg aaagcagagg aagatcggat cgccttcaac cgttacattc agcagatcgg
    13321 cggtcgtgcg gttggaaagc gagatcgatt tcgtcgtcaa ctgatagctt tccagcggcc
    13381 agaccagctc ggaagcgttg acaatgctcc ttccggtgaa ggatgccggc agaaaactga
    13441 gcgtccggcc ggccgatgtg atgaatctcc gttcccggtg aacgctgtac agggactcat
    13501 acttggtgtg ccaggcaagc ggaagaccgt agcgaccgaa gagcgaatac gtctgaagaa
    13561 gatggtccat ccgatacccg ccgccgccga tgagagtata cgttgtcatc ccccgctcca
    13621 ctccgatcat caaggccagc tccgtatcgc tttcaagctt catatcatgg tctttgtgaa
    13681 tgatttcgcc gacgcgctcc ggctttattt tagcggaatc aaaatctccg acccagatat
    13741 cggcaaacag gtcgtgtctt accgcgaact cgatgccgct gtcggcgacc acgatgaacc
    13801 ggtaggcttc gagatgaaga gcttcaagag ccttcggcgc tccccctccg gtgataatca
    13861 acacatggct catcctcttt ccctcccttt cgcctttcga tacacttacg cacaatagca
    13921 cacaataaag aggataccaa tgcctacgac gcttaagcga ttagcaacat atggcgttca
    13981 accgccgacg atcctgattc cgaaacacga gatcgattta aagaagtggg cggtcgtcgc
    14041 ctgtgaccag tacacctcgg agccggagta ttggaaacga gttgaagcat atgtgggtga
    14101 cgctccttcc acactgaagc tgatctatcc cgaagtatac cttgaagaga agaattccca
    14161 agaacgaatc aatacgatcc accaagcgat gaatcgatat cttgaggccg atctctttga
    14221 tatctacgaa gagagcttct tcctcatcca tcgggaggat gaaggccgct cttccggtcg
    14281 atggggactt cttgcggcgt tagatctgga acattacgac tggaagagcg gctcgcatac
    14341 gctcatccgg gcaagcgagg aaacgattct cgatcggatt cctccccgca agctgatccg
    14401 gaccggcgcg gtcttggagc tgccccatat cctcgttctg atcgacgatc cggatgagac
    14461 gattatcgaa cctttgagca ccaatcggtc ttcctttcat caggtgtact ccacttcttt
    14521 gatggagggg ggcggaacgg taacggcgtg gcggatcaac gatgaacagg agttcagtcg
    14581 tttagcggat cggttccagg cattgctcga gcggcaatac acgaacaacc cgctcctctt
    14641 tgcgatgggt gacggcaacc atagtctggc gacggcaaag agctgctggc ttgacatcag
    14701 aaaagggttg tccgaagagg agatttccaa tcatccggcc cgttgggcgt tggttgaagt
    14761 ccaaaacatc tatgatccgg gcatcgtctt cgaaccgatt caccggctgc tcttttcaat
    14821 cgagcatcaa tcatttcttg atgcccttgg aagcttctgc acctcctacg aaacaaaaag
    14881 cctcctctcc atcgaggagc tggaccgctg cctcgccttg gatgacggtg tgcagcggtt
    14941 cggatataag gacgaggagc gttttctcct cttttcgttg aaggatccgg cctcctcgtt
    15001 ggccgccgga accatccaac acgtgatcga ccgattgttg gatcagcaat cagcgaccgt
    15061 tgattacacg cacggcgaag atgtcacgct tcggctggga tcgcttccgg gcaacgcagc
    15121 ggtcattctt cccaattttc ccaaatcggc gtttttcaaa accgtcaagg aagaaggccg
    15181 attgccgagg aagacgttct cgatgggaca cgcccatcaa aagcgcttct acctcgaagc
    15241 ccgaaaaatc accctctaaa gcggcgcctg cctatgacat aggcaagcat gcctgcagga
    15301 aatgcgggga gcaacgccca gagggccagc acactccgcc cgactccttc aaagtactcc
    15361 cgcctgcctt caagcggccg gtcttcaaga atgtcggggg attcgcgctc ttccaagggg
    15421 aagtagtata cgacctctcc ttcactttgg tagccgtact tgaaggcggc atcccgaagg
    15481 gcatcctcct tgcccatttg agcttcctgg cgttcaaggg cctgtatttg aatctgaaga
    15541 aggttttgat tatacgaaag acgctgaatt tccgcttcga gtgctcggtt gtgcaggtat
    15601 cctccttgac cgaaaacagc cagaagaagg agaaatgtga aaaaatacgt caataaaccc
    15661 gccagcaggc ttctcttaag catataaatc tttaatattt gtggtaaagt atgatactat
    15721 gggtattata taggaatctg accttgttgt ggaggtagga atgtctgaaa agaaacggta
    15781 tgggcgccaa atttttataa gtttcattgt cacgattgtc atcctcctcg ggggagtgta
    15841 tctggcaacc cagtatctcc tgccgcacgt aaagtgggat caagatcaac tgttttcttt
    15901 cttcgtgaag ctgtttcccc tcctcatcgg cttgatcatg attgagatag gtgttgtcat
    15961 atctaagaag cgggatgagg atttcgccga cgatatcgac aagcttcccc ccaacgccta
    16021 cgatcgacct ttctatgccc tgcctcagga tgatcccgcg tttatccgaa acgatgaagt
    16081 aatctacgcc caagctcccg tccttgaaga agcaacgttc tccgaagcgg ttctcagtga
    16141 agagccgatc gttgaacggg ccgccgaagt gatccttccc ctcgatcttc cccaggtcga
    16201 agaagagccg gaggttctgg tcgtcgagcc ggtcgccgag accgtcgcaa ctgagcttga
    16261 agaaccgatt ctcgagaccg tcgcagcaga ggaaaaagag ccggtcgtcg gagaagcggc
    16321 accggttgtt cccccctatc aagcgccgcg tgacgagatt tccatcgcca tcgaggaagg
    16381 aatccttcaa gtgtacgaga tggatttcga ttcgatcctg gagcttgagc ttgagagcgc
    16441 gaacgatctg aactatgacc tcaccgtcgt gatcgttcgg gttaccgaag gaccggttga
    16501 cgcgatcgct cagaagctga ttcgccaaag cggggacctt tcctatacat tcacccttga
    16561 ggacaatacc atcggcatca tcctcccctt ctacaacggc gaggaagccc gcaccttcac
    16621 cctttccctg atcgaatcct gccaacagga gttccaggga agcgaattgg aaatcgggtt
    16681 cgcatcccgg ggcggtcgag tcgtcgaaaa ggatgaaatt actcaggagg cgtataaagc
    16741 cgcttcgttc taatctttca acggaagaga aaaccgcatc cggtctttgc tcagcaccgt
    16801 gttggcaaag acctttttcg catccttcag cagatagcga agctcataat cggagtagcg
    16861 cggactgtag tggagcaagc cgagctgttt cacattcgcc tccttggcga ttcgggcggc
    16921 atcggatgcg gtcatgtgct tcttctctcg ggcactctcg accaggtcgt cggtgaacat
    16981 cccttcgcag agaaagagat cgctctcttt gacatattcg gcgatcctcg gtgaatagag
    17041 cgaatcggta acgtagctga acttccttcc ttcccgcaaa tcccccatca catcggaggg
    17101 ctgaatgacc cgcccgtctt caagggttac cgacccgcct ttttggagga ttccccacat
    17161 cggtcccatc ggtattccga ggctttgagc aacttccgga tggaacagtc cggggcgttt
    17221 cttttcttca aacacatatc cgaagcacgg cttggtatgg ttcagctcga acgcccggac
    17281 gacaaattcc tcctcttcga ggatgacccc ctcctccacc gtttttacga tgatctcata
    17341 gttgatgtag atgtcgagaa tccggcgatt cgcatccacg tattctttga gtctgcccgg
    17401 accgtagaga gtcaatgggg tatcgcggtc gacctggctg gaaagcatca gaattcccgg
    17461 cagccccgtt acgtggtcgg catgcatatg ggaaataaag atcgaattga tcttcttcca
    17521 cttcaaattc agctttttca gcgagacctg cgttccttcg ccgcagtcga agagaaacag
    17581 ttcgccctcg cggcgaatca tcgcactggt gaggttgcgg ttcggcaagg gcatcatccc
    17641 gcttgtcccg aggacaaaga cttcaaaatt catacccatg agtatactga actttagtgt
    17701 ttaatcaaag gcaagagagc cgatcgtttg ctttcggagc gcccggtgga ggctgaaaaa
    17761 gacggtagcc gtgctggaaa gaatcaccaa tgctcccaga aggagaattc gcttgatcgg
    17821 aacggcgatg gaaaagcgga gcaaatagaa cgagaaaagg gaaaaagact gctgttcgat
    17881 caggggaaga accttcggca ccgcatagga aaggagtatt ccccctaaga ctccgaagat
    17941 cagggcgatg atcgtcacga tgaggacgct gagcaggtac gagcagccaa gcatccgcct
    18001 ccgggcgcca agcaacagga gcagggttat ctgccgtttg tcatcctcca ccatctgtcg
    18061 ggcaagctcg ctggtcgcat agccgagcaa caagccgatg atgctgatga tgatgaaggt
    18121 gacctctttg ctggtgatga gattgcttgc aatctgaggg gcttcatcgc tccacgttgt
    18181 gacctgatat ccctcatgga tcagtcggtc tctgacttct ccgattcgat ccatctcgac
    18241 aagcacttca tggtgaaacg tcaccgattc tccgcaaagt cgaaccagtg aagagaggtc
    18301 ggtgaagacg agcaactcat caagctcctt gtagccgctg tcatagatcg ccccgatgcg
    18361 aaccaacgtc ggacggacgt tcgcttccgt cccgatcatc agtgcaaggt tctcccccgg
    18421 gtgaacgttc aaggtttggg caaggctttt gctgatcatc actttcatca aggttgtgct
    18481 ttcactgctc tcctttagag tcacaagctc agcttttcgt tttcccgtca gataatcatc
    18541 ctcgacacct ttgagaaaga ccaaagcggt cgtctccttg gagtaaagca acccatacgt
    18601 cgttccgaca agatatgaat cgtatgaacc gctcgtggga aatgaccggt cgtggacgat
    18661 gagatggccg ctgttgatga gcgcatattt atttgcaatt gcatcgctca tcgaggtgac
    18721 gaagatcaac gcgaagacca aggcgcttaa agaaagggtg atcagcaaga tctggaagag
    18781 aatcctcctc ctgcttgccc tgtttccgac catgccgaga gaacgtctta ttaagagagc
    18841 ggcgacctct ttgctcacat tccctccacc agcgttcgct cgcgcaaggt gagaacacgg
    18901 tcaagcttgc ttgcgaactg cgggttgtgg gtaaccagca gcagcgtcgc gtttcgttcc
    18961 ttcaccagac cgaggagaag ctcttcgatc atcgccgcat tttcctcatc cagattcccg
    19021 gtcggttcat cggcaaagat gatcgccgga ttgttcacca acgagcgtgc tatgacgacg
    19081 cgctggcgct cccctcccga aagcttgtcg ctggtatggt ggagccgctt ctcaaggccc
    19141 acccgatgaa gaaggtcggt tgccgctgca tgtgcttccc ggggggaaga gccggcgatc
    19201 agggcggcga tcttgacgtt ctccaacgcg gtgaaatcgg caagcatata gcttgcctga
    19261 aagatgaacc ccatcttttg tgcccggaaa atcgccttct ttcgatcatc aaggtcgtgg
    19321 aggagcaccc cgtccaacat gaccgtcccg ctgtccgctt caagcaggcc ggcagcgatt
    19381 tcaagaagcg ttgattttcc cgagccgctc ttaccgacga tcgccacgct ttgcccgtgc
    19441 ttgatcacca atgagattcc ttcaagaatc atcaggcggc tgtcgccggc ctgcgttgca
    19501 ggaaatgatt tcgtgattga agaaaggtgg acacattcaa aggagttcgc catgacctag
    19561 tatctccatg atgtcaacgt gcagcaggcg cctggaaccc gtataggcgc caaaagccgc
    19621 aagcgcaagg atgagaacac acaagaaacc gacctccccc gaccgaagag caagggtcag
    19681 atcgagaccg aggttgctta agatatagac ccggagcatc gggtacagaa gaaagaatat
    19741 tccggtaagc gccagaccga ggacgcagcc gatgagggcg acgatgagac cctgaagaac
    19801 aaagatgtgc ggcatctttt tttcttgaaa cccgagggtc ctcagaaggg cgatctcccg
    19861 ctgcttggat ccgaccagcc gtttggaaga gacgtgcaca tgaacgatga tgacgagaat
    19921 caacagaaac aaaaggagcg tcatcatctt ttgctccagc tccatcgctt ggtacaagct
    19981 ttgattggcc tctttgtacg agatgacgtc atacgggctg agctgttttt cgacattcct
    20041 gatcgatcga tcgcttgcat ggatgccgat cgaatacgct tcactgccga tgagggaggc
    20101 aaggtactcg ggggtgacga gaattgtcgc gctgtcaaac ggtccgtact ccgtcgcata
    20161 gattccggta accggaatgc ttcgttgggt gggaacctgt gtgacctgtt tcccgctccg
    20221 taaaaaggtg atggttacgg ggtttccaag aaaccgattc cgataggaaa gagcgacacc
    20281 ctccgacgta aacagctcac cggcgatcgg atggatggga agagtcgcac ctacatcgat
    20341 cgcccggatt cgcattggat ggttttcacc tgtccggtcc acgaccagaa cgggaagatc
    20401 ctgatagaca aacgcctcct tgacgccgga caacccttca agctcctttg ccaaggctcg
    20461 cccgtcctcc aaggaatagc gttgaacaga gtggaaatca aacgattcgt aggtgcggag
    20521 atgatcgaac tgggcgttct ggagcgcttg catgaagctg acggttacta aaatggcgag
    20581 aatgccgatt ccaagaccga cggcgattcg aattgaaacg ccgcggtgcc tgttcgcttt
    20641 tgagaacgca tatcgccagg caagcctcag gatcatcacc acctctcaat aatagcggat
    20701 tgacgtaacc cgcattccgc cggccgccca tgttacatcc acgtacggcg ttccttcccg
    20761 atcatacagt gtttcagtat acccgaaatc ctcatggtag cgggtatctt ttgtcggcac
    20821 tccgttttgc agctcatgac gaagaaccaa agttcggtcg tcatcataga tatcaaccat
    20881 catccgctcg gagggatatt cggtgtgaac ttctttcaat cggccttcca aggtatagta
    20941 gaacgtcctt tcatactcat cggtgtattc cttttgcacc aggccgcttc gggagtagag
    21001 ttgagtacga tcatcctgaa ccaagcgcaa ataaccttcc tccgtctcat cgatcacgac
    21061 cggagaaagc tcttcttcct ctttccacgc atcgctcatc ctcaatgacc cttggctcac
    21121 gtagcgctga ccgccctcct catccaccac ggtaacggta taccggtcgg gttcacggac
    21181 gaaccgggaa aggaccagcc cctcttcgcc caatcgggcg atggagatga gattcggctt
    21241 gccccggtca taggagtagt aggcaagcgt aggttcatcc tcccgcacgg ccgtcgaagc
    21301 gatgatgagc cgcccggcat catccaacgt gtaggtgctt gtcgttgagc cttcctgttc
    21361 tttcacaagg gagccatcgg taaacgttcg cttttggatg cgtccgtccc gataggtccg
    21421 ggttgtgatc accgttgccg gttcgatgta ctggtcgatc tcgacccata cctgtttgcc
    21481 ctcgtcatag agcaccctcg aataggaaga gagttccaag cgatagcgat cgccttcctc
    21541 atatcctccc aacggctggc cgatcgagtt cgccttcatg ctggccccgg caagcgggct
    21601 cagcatgatg agcagaacac cgattaccac cgcctgtttc atgcaagccc caccagagaa
    21661 tcgagaaacg gttccgcttc aaacagttcc agatccccat acttctcccc gactccgaca
    21721 aaagagatgg gaagacccaa ggaatctccg atttggatca gagctccgcc cttggccaga
    21781 gagtcgtatt tggagagaat cagcgcatcc aaccggatcg cctggtgaaa gatcgttgct
    21841 tgactgaggc cgttctgacc ggtggtggaa tcgatgacga gaaatttctt gtaatgcacg
    21901 gaatcgatcc ccttcgtctg cacgatcttg tcgattttcg ccagctcccg gaccagatgc
    21961 tccttgttgt gcatccgtcc ggcggtgtcg accaggagca gctgatcgcc ccgggcccgg
    22021 gcgcttgtga tcgcatcgaa gaccacggcg ccgggatcgc tgccgctctc ctgcttcacg
    22081 attcgaaccc ccacccgatt ggcatgaacc tccaactggt cgatcgctgc agcccggaac
    22141 gtatccgctg ccgccagcgt gacggaatac ccctgcgttt tatacagccg ggcgatcttg
    22201 gcgatcgtcg tcgtcttgcc gaccccgttc acgccgagga tcagaaatac attgagggcg
    22261 tttcgatcga tcacgggatg gaacgtatgg accatcggcc gaagctgttc cttcaccagc
    22321 agctgcagct cccttgtggt caggtctttc tttttcctga ccagattccg cacctcatcg
    22381 ctgatctgca tcgtaagctt tgaacccaga tctccttcaa tgaggagttc ttccagctct
    22441 tcgaagtatg attcatcgaa tctctttatt ccaaacaagc tcttcaatcg agcaccgaat
    22501 ccttttgcca tcgctatctt ccttattcct gcatcatacc aactgccgag gcgtatgagg
    22561 agagctcggc gaccatcgcc acgcttgata caacgctcaa caagcgagtc gaaccaaggg
    22621 ccaatgacca cagcgggttc tcatgcttgg ggtcatatgc agccaccaac gcttgtgcag
    22681 caacataaga accgaaactc aagatggttc ccagcgctcg acgatatact ctcttttgtt
    22741 catccggcac atccaccgaa gcttttcgca ctccgaagcg gatgacagtc cccgtttcgt
    22801 gagggatgtg aaggacgctc ttcacaaatc cctctttttc cactgaaaga gcgatcggat
    22861 aggagggtga tcccaaggtt atcatgccgg attcgcccgc ctcgccctct gaggaatagc
    22921 gtaccgctcc gacgttcgaa agcaaggtga ccggctcgtt aacgatccgc tgaaggtccg
    22981 gtcggatcgt ggcgacttcc ccctcctccc accgaacggc gaccttccga ggttcatggc
    23041 cgcttcgctc cacggtaacg gtagatttat ccgtcggcat gagatacaca tccaacgatt
    23101 cgattctctc catcttctcg gcaatgcgga tctccttcga tccctcgatc ttcaaaaccg
    23161 ccttcgtgcc gtcggtgaga atgtgtacgg cctgagccaa caactcctcg atcagggtgc
    23221 gctcttccat ggtgaccagc aatcggtcat ggatcaatcg aaggtccaag cgaggtcctt
    23281 cctgatacaa gagccggacg cgcctagctt gaccatacgg cgtcacgtcg acaagaaaca
    23341 ccccgagggc ccgttcccgg ctcatatacc aaaagagggc ttcttcccgt tcaaacagcg
    23401 caagaagaga aggatcgtac cctttgcttg cataggtgct cggcagatcc gatccggtat
    23461 cctcaaacgc ctcccctttc ggttttgccg gttgcttctt tgcatatgct tgggcttgtt
    23521 ccgccaaaaa cgcccgatcg atctcctcct gatcatactc cttcaggatg gctttccgat
    23581 catccgtcag gacgacccga ggcacgccct cggccagaag cgattgaagt tcaaaaacaa
    23641 tgggttcggc cgcctcactt ccggcgatga tgaggatggc aggggtttgg gcgaacaaca
    23701 accctcctcc tgcaagcagg agaagaagaa ggatcatcga acccttgcct ttcatcgctt
    23761 actccgaatg ttgccactgc aggtatgctt cgatgaatgg atggatctgt ccatccatca
    23821 ctgcctgaat attaccgacg ctctcattgg ttcggtggtc tttcaccatc gtatacggct
    23881 gaaatacgta ggagcggatc tgactccccc aggcgatatc cttcttctcg atcgcatttt
    23941 tcgcccgctc ttcatcccgg atcgcccggt agtattcata caagcggctg cgaagcatct
    24001 tgaaggcaac ctctttgttc atgatctggg aacgctcatt ctgacattga acgacgatcc
    24061 ccgattcaaa gtgggtaatt cgaaccgcgc tgtcggtctt attgacgtgc tggcctcccg
    24121 ctcccccggc ccggtaggta tcgaggcgat aatcctccgg cttcaagtcg atctcgatct
    24181 catcatcgat gaccggagag gcgtacacgc tggtaaagga ggtgtgtctt cgtttttggg
    24241 aatcgaaggg gctgatccgc accaggcggt gaactcccgc ctcccctttc aaatatccga
    24301 agctgtacgg cccggagatt ttgatggaag cgcttttgat tcccccctca tcctcctgga
    24361 gatcgaggat ctgggtcttg aaatccatca attcgctgta gcgcaagtac atccgcatca
    24421 gcatctgggc ccagtcactc gcttcattcc ccccggcccc ggcgtggatc gtcaagaacg
    24481 tatcgttctt gtcgaactcg ccgtccagaa gggttttgag cttcagcttt tgatatgtat
    24541 cggctaccgc ggcgatgtgg gcatcaacct gttcctgctc ttcggcgttg tccggctcat
    24601 ccgcatagag ctcgacaagc tcggtgaggt cgtcgatctc ttcgatcagc tccttccagg
    24661 gctcatagga gtctttcagg gaattgagcc ggctgaacgt ctcttcggcc cgcttccggt
    24721 catcccagaa tgtcggttgg agtgtccgtt cctccagggt ttgaatctct ttccggattg
    24781 aggagctttc aaagacgcct ccaaacggta taggcttctt cttttacttc ttccaggagt
    24841 gctttatttt gaaaaaacat ggtatgtcct ctttccatct atttccgttc aatgcgcaaa
    24901 cccaacgtga tctttcgctc ccgggcggga gcaagggcga tctcgtaggc gcaaagcaac
    24961 gtagtgtgtt gataatgacg ctgctcacca tgagcctcgg caattgtaca atcttccttc
    25021 accaaagaaa agcgggtatc gcttaccagc gtcagctggg tgtggttcgg ttcatcatag
    25081 atcttcaacg atttcacctg agccgacgaa aactccttct cttctttcat gacggacttc
    25141 ctgttgtcca taaggatgac ggaaaccgct tcttttggcg gtgagagagc caacactagt
    25201 tcggttccat accggaacag cagctcttct tcaccgttgt tggtcaaggt aatatccaga
    25261 agaacggtgt tttgtcgaat cttatagtgc ttgatgaccc ccagctcccc tccctcgaag
    25321 ggaccttgat cacaaacggt tcgaagcaca tattcggtcg atcggcggtc gtagacttcc
    25381 aggccataca cccgctcctc caagttcagc acgttctctt tatcccgctt gtcatactcg
    25441 tcgaccacat agttttcatc gaagaaaaga tcggtgaaaa tccgctgttt cactccgggt
    25501 ttcgggggat gaaggagatg atccgtcgtc ccgtagatcg gaagcgggtt aaaggaatcg
    25561 ccatagttgt gcaatgacgg aagatacgtc aactcgctga gcgccccgcc ctttgaatcg
    25621 atcacgcagg agagaaactt gctgattaca aggtgttcgt ccagtccgtc atggtcgtag
    25681 tcgctgacat tcggatagga gaagtccttc aatgaggcga gaatcgaatc gatctcatgg
    25741 atggaacgcc aatagagctt tctcacctga ctgtacagaa acgtatcata cgcatcgccg
    25801 atgtagatgc ttcccaccga ggctcgactg atcagctgtt ccagccgccg tttcacatcc
    25861 cgatcttttt tataggaacg ggcatactcg ctcgtcgtca tgtagcggcc ttgaaggtaa
    25921 tacagcgttt catcctgcac gaggagctca ttgaaacagc aaagcccttt ccgttttata
    25981 tcgtgtccgt accaacccat cggaaggtat ccccgcttca gcggcaactc ctgtccttcg
    26041 atctcttcag tggaggagct ccccttttgg acaagagcgg aaaacagttg acccgtctct
    26101 tcgggggtga ttccacctgt ggaaagctga tcgaaattaa gcagcgccaa gacaaacctt
    26161 cgatcgctct caaccacctg cctgactgcc gaatgcagtt tggaaaacgg tatcgccttc
    26221 ctggaaaacg cttcaacgct ccggctgacc acatcatcga ttggcaggat gattccccgc
    26281 tttccaagct cctgcatgat gaccggttca tcaaaatagg ttttctcgat gcacggatcg
    26341 tgggcggaga ccatcaaggt atcgagaaag cagagattca tcgacccgat atatgcgggg
    26401 ctgaaaatct ggtggggaca gaagagggtg cgcgaacgtg tttgataccg tttgcgcata
    26461 tacgttgtcg tctgctcgat ctgataggcc cgatccttcg gggccaagag cgaagggatg
    26521 ctttggaaat acgcgtgcga gagaaactca agcttttcat ttcgacagag atcggcaagg
    26581 agcagattca ccgaaggatg gtttttctcc aaaaactcca tgaacgggac ggagagcgca
    26641 agctgaaaca ccacgttctc acttcggttg agttcagtca acaggggaag caacaccgct
    26701 tccagcgccc tggtaaacaa aggcgccgga agaccatgag ccagttgagt atatccaccg
    26761 attaaccgtt tcatccctat agcttaacga tgcacttggt gggacacgcc tcggccgcat
    26821 cagcgatctg gttgtgttca gccagctggt tgaacgtcgc caaaaagcga tcaaccgtac
    26881 atcccgactc aggatacttc gtttcacata tcttacatcc aatacacccg acagtacagt
    26941 atttccgcac tcttcctccg ggttcatggt tattgcattc aacgacgtat tcatcatccg
    27001 cacctatcat ctgcatcgca ccggtcggac acaccagaac acatttttca cagctgatgc
    27061 attttttttc atcgacgatg atataccctt tttcatcctt ggagatcgcg ccggtcggac
    27121 agacattgat gcaggaaccg aggcgcaggc aggcggattt gcacgtcgta tccccgccga
    27181 gcagcagcgt cgccgcgttg cagtcctcaa gaccctcata ccgataatcc cgcttcgtga
    27241 cctcgggatt cccccgacac atcacatgag cgaccattcg cctcacctca tcgacggaat
    27301 ctactttcag acccatgatc tcgctcatct tttcgacaag cgagggaccg ccgggagtac
    27361 acaacccaag ctccgcctca cctttgacga ccgccgccac atacgcgttg catccggcgt
    27421 agccgcatac cccgcagttt gctccgggca tgatcggctc aagggcaagc actttcgggt
    27481 ctttttcaat cgccagcttc ttttccgcat atgagagacc gtatccaaat ataccgccca
    27541 agacggctac aatgattatt gctagaagaa tattcacaca atcctcctat accaagtgca
    27601 gattcgtcaa cagtgatttg tcgaacgcca tgaacgcaag cgccatgagt ccggcactga
    27661 tgaaggcgat cggcatcccg cggtacgctt ttcgcaccgg atgcagatca agtcgctcac
    27721 ggacattgct catcaatacg atcgccaatg tgaagcccag tcccgcagcc aaccctgcgg
    27781 tgaatgactc catcacattg tagttgtttg taatattgat gatcgcaatg cccagaaccg
    27841 cacagtttgt cgtaatgagc ggaaggtaga tccccagcgc cttgtagagg ggggggctca
    27901 ccttctgaat aaccatctcc aacagctgaa ccaaggtggc gattaccagg atgaacgtca
    27961 acgtctggag ataggcaaga ttgaaaggaa ccaacaggaa atggtagatt gcccaggtga
    28021 cgacggaagc aaccgtggtc acgaaggtaa cgctcgctcc catcccgatc gcgttctcgc
    28081 tgttcttgga aactccgata aagggacaga gacccaaaaa ttggaccaaa ataaagttat
    28141 taatgaaaat aaaggtgatt aagattgcaa tataactcat atcagcctgc cttcctcata
    28201 gccttccagt caaagaacgc tttcaaaaag cccatcaata aaagggctcc cggagcgagg
    28261 gccatcacct tgaaggggtt gtcgaccagc cacggcacct caatcactcc gctgaaggag
    28321 ccgacgggga acaaggtgat cgttccggcg ccaagaacct cgcgaatcag ggcgatcagc
    28381 gtcagactca aagcgaatcc gatcgacatc ccgatcgcat caagggcgga atcgagcacg
    28441 gtattgttcg aagcgaacgc ttccgcccgg ccgaggatga tgcagttcac gacaatcagg
    28501 ggaagataga cacccaacgc tgcatagacc gccggagcga aggcatgcat caacatctcg
    28561 atgatcgtca ccaacgaggc gatgatgacg atgtacgccg gaatgtgaat cgaatcgggg
    28621 atgatgtttc tgagcgcact gatgatgatg ttcgaaaaga ggagcacaaa gagcacccct
    28681 gcgctcatcc cgatcgcgtt ggagacctga gtggagaccc cgaggacggg gcacaggccc
    28741 agcacgatga tgaaaaccgg gttttctttg aagattccct ttgtcaattc tttgatgttc
    28801 atcacttgcc tcccaacctg atcacatagc gcgatcccgc ttcgagcgcc ttgccgatcg
    28861 cgacgaacgt caccgtggaa ccgctgaccg cggcggcatc ttccgagttc agcatgctct
    28921 tgttcgtcgg aaccgcctgt gccgcgccgg tcccctcgaa cttgtccata tatccctcgt
    28981 tctccgcttt ctttcccaga ccgggcgttt cgttgtggga aagcagctga acacgaagaa
    29041 tctccccgtc tgttttgtag gaagccacca gcgtcatctc accgccgtac ccggcgcctt
    29101 tgagaccgag gatatatccc gccgtccgtc tatcatcctt cagttcgatc atgaaggtga
    29161 ccgtcggatc atcggttaca accttctggg ctccggcttc ataccccatg ctcaccgctt
    29221 cgagacccgc gagccggctc gcctgtccct gccgttcaat gaccggggcg gtgatcgcgt
    29281 tggtaagggc cagcagagcg gtgctgatcg cacagatcga taaaaggatc agcgaatact
    29341 tcagattctt cttcatgcca ccgcctcctt tcgcgcaaag agtttcttct ctccgaactt
    29401 cctcggtcgg atatagcggt cgatcgtcgg ggtgatgatg ttcatcaaaa ggattgaaac
    29461 gctgaccgct tccggcatcg aaccgaagct cctgaagaga aaggtgaaga agccgcagcc
    29521 gatgccgaag atgatcttgc ccttggagga gaccgggctg gtcacgtagt cggtcgccat
    29581 gaacagcgcg ccgaagagaa gaccgccgct gaacatcgac gggaagaacg tcccctggaa
    29641 gatccccaga ccgagcggaa gcccgccgaa gacccgcgag aggagagcgc tcgaaagcag
    29701 ataggtaacg gggatatgcc acgtgatgat cctgcggatc agcaggtaga tccccccgat
    29761 cagcaagaga agcgcactga cttcaccgat tgatcctgcg acatttccca ggaaggtatc
    29821 gatcgtgtac gcattgattc cgagaaccgc gccgatcttc tgtgcgattg gcgttgccgg
    29881 atagcccacc caggaaagca gctcgttggt ggtggctccg cacgaaccgc ctgagaccgc
    29941 cgtcttcatg aatcccagcg gggtcgcccc gctgagggtg tcgggcatcg cctccagaag
    30001 cgtccgggga gtcgtgaagg tactcatctg ggtggtgaag ctgaagaaga cgaagacccg
    30061 gccgctgagg gcggggttcg cccagttcgc tcccagtcca ccgaacgtcc acttggcgac
    30121 gaagatcgcg aagaaggagg cgacgatcgg aatgaagagc ggaatcgagg gcggcatgtt
    30181 caagccgacc agaaagccgg tcaggaccgc cgacccgtcc cagatggtgc actccttgcc
    30241 gatcttcccg agcaggaact ccgtcaacgc tgcactcacg atcgaaacga gaatgatcac
    30301 aaggctgtac agtccgaaga cgtagattcc ccacaacgca gccggcacca gagaaagcga
    30361 cacgtgccac atgatgttgg cggtcgaact cccgtcgtgc aggtgcggtg atgaggatac
    30421 ggtcatcaaa ggttgttcag tcatcatttc ttcctcccca tcttcttgcc ggtcttgaag
    30481 gtatgaacaa gcggcaagtg agcgggacag acataggagc agcagccgca ctccttgcaa
    30541 tccatcaaac tcaaggaatt catcgcaacc tcgtagttgt ggttggtgat ctgcttgtac
    30601 agcagcgtgg gcaccaagcc gatcggacag gcatcgacgc actttccgca actgatgcag
    30661 ggagtctgac gggccttctt ctttgccgca agcgccagaa ttccgtcgga tcctttcgtt
    30721 accggagttt cggtatcgaa aaaggcgaag cccatcatcg gtccgccgat gataagcttt
    30781 tcgacttcgc cctgaagacc gccgcagaac tcaagcagat cgctcacttt cgttccgatc
    30841 ggagcaagaa cattcttcgg attgacgatc ccctcccccg ttacgctgat gattcgttcg
    30901 tagaggggtt ttttatagac gatcgcctca tagatcgcat agcaggttcc gatgttggaa
    30961 acgaccgctc ctacgtcaat cggcagtttt cccgatggta tttcccggtt gatcgtggct
    31021 ttgatcagct gtttctcatc gccctgcggg tatttcacct tcagtccgac gacctcgatc
    31081 ggccacgctt cttccctgat gatttgtttg aggtgttcga tcgcttccgt cttgttcagc
    31141 tctacgccga cgatgatctt cttcgcttcg agaatctttg cggcgatcaa cgccccttcg
    31201 agcgcttctt ttcccttttc cagcatcagg cgatagtcgc aggtcaggta gggttcgcaa
    31261 tcggccccgt tcaccaagaa gtattccacg tttttgtccc ggggaacggc atacttcacg
    31321 tgagtgggaa aggtcgcccc gccgagcccg acaatcccgt gctccttgac ggaggcaaga
    31381 agttcctctt ttttgagctt cttccaatcc accggtttgg gcgtatcctt cggttgcttt
    31441 tcatcgaccg caatcacgaa cgcgtcgcac tgcacggaat tcgccagcgt gacggtcttg
    31501 atttccttga tcgtcccatt gaccggcgag tggacgtcag cgctgatgaa accgacggat
    31561 tccccgattt tctcccctct ttcaaccgca tcacccttcg ctttgagggg ttttgcaggg
    31621 gcgcccagat gctgggagag agctactacc agctcgctcg gcagcgggag gcggacaatt
    31681 tcctgctcgc ggctgaatac cttctggtca ggtgggtgga ctccaccctt tctgaacgta
    31741 ggtactgcat gcatatgaaa cgctccttat cgtaaatctg gtacggatgt atgttctgtc
    31801 tgatgctttt atgcaaatac tgttctattg tacaggaatt tcagtataat ggaaaccatg
    31861 aatcgtattt ttaaatacgc gttttggaaa ctcaaacgaa cgaaggtcta cgctcttgtc
    31921 ggtaaaagcg gtacgggcaa atcgtttcgt tccaaaatac ttgcagagcg ataccatatc
    31981 gactacatca tcgatgacgg cctcctcatc aaaggagacc ggatcatcgc cggaaaatcc
    32041 gccaagcggg aggagcatat cctcgcggcg gtccgaaccg ccgtcttcgg cgatgaagac
    32101 cattaccttg aggtccttac cgcgctcaag aaagaaaaag tccataggat cctgatcatc
    32161 ggcaccagtg aaaaaatggt gtacaagatc gccgagcgcc tggagcttca gccgcccgag
    32221 cgaatcttcc atattgaaga gatcgccacc aaggaacaga tcgagacggc aatgcggatc
    32281 cgctacgcgg aaggcaaaca cgtcattccg gtcccttcca tcgaaatcac ccgcacctac
    32341 ccccaaatcg tctatgactc gatgcgggta ttccgcagag cgaaacaatc gaaagtgttc
    32401 aggaagcaga acgccggctt tgaaaaaacg atcgtccggc cggagttttc caaatactcg
    32461 aaaacctccg tcagcgaagc ggcgctgacc cagatggtcg cccattgcct tgatcaattc
    32521 gacagccaga tccgggttac agcggttcat gtcttaagcg aaagcgacgg ctatgcgctg
    32581 agggtgaaac tccgggttcc ttcccatcat cagattacca cgagtttgag agagcttcag
    32641 gaatatatcg ccgactcatt ggaacggtac ggcggaatct tcgtcttcag cgtgcacatc
    32701 gaaatcgaag agtggggcta ggcctctttt cgttgcttcg ccggcggtct cttccgtttc
    32761 ggggtggtag gcctggtcac cgggctcgcc ccgcccctcc gttgcggttg cggcggacgg
    32821 cggaccgcat tccggggagt ccggaatccc cgttcggaga gaatccgcac cgcggcgagc
    32881 tccacatcat agttggaggg tgtcatcggg ctgatcagca ccttgatctg gcgaaaggtc
    32941 tctttgaagc gattggtcgg actcatcgag gcgtcgctga acacgatcag ggggtcgacg
    33001 agcgccttga gcatctcagc gaattcgatc tcttccccat tgttcgtttt ggcagcctga
    33061 acccggctgt ccagccccct gatgctctct cttacctgcg gataggagca gtagatcgtg
    33121 aacgccggca tcatgaaggg aaggatctta taccggttaa ggtctgcgaa aatctccgcc
    33181 gcatggccgc ttgaaaggat ttttacaagc tcctccgtca ggcgggagga ggagatccgt
    33241 gaaagctccg gagcgtattt cctgagtgcg acgcggagct tgaagcgcaa tgaaaagccg
    33301 gtaatgaccg cgtatttcac cgcccggatc atccgaaccg gatcttcggt gaaggactcc
    33361 tccaggggga tcacgctgct gatccgcttt ttcctcatat cctccatcgc gtcgttgaaa
    33421 tccagcaact ggccgttgga tggatcgtag taaaagctgt tgatggagaa atcgcggcgc
    33481 ttggcgtcct gttcgatcga accgaagacg ttgttcgatc cgtcttccgc ctcttcgccg
    33541 ctgcggaacg tcgatacttc caggactttg tccttgaaga tcaagtgaac aagcttgaag
    33601 cgccggccga tgatccgggc attgtaaaaa agccgttgga cctgccgagg cgaggcgctg
    33661 gtggcgatgt cgaaatcttt ggggatgttc cccagcatca gatcacggac cgccccgccg
    33721 acgaggtagg cttcggcccc gctgccctga agcttcttga tcacccacag agcatcgcgg
    33781 tcgatgttcc gggtttcgat cttgtgttcc gcctgggtat agatgttggc gatcggaagg
    33841 gtgttgccct ggtcatcttg tttatatctt atcagcatcg gtatctcgaa aattgggagg
    33901 atattgcagt gaacgaatga ttcgtattac actctttcta caccatccaa cgac
//
LOCUS       DAOSLI010000017        31829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1606_length_31829_cov_32.321961, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000017 DAOSLI010000000
VERSION     DAOSLI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 31829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31829
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1606_length_31829_cov_32.321961"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PLF67_03985"
     CDS             <1..1241
                     /locus_tag="PLF67_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE89207.1"
                     /translation="LSETGRRMFFPKGIVAQSAEATEKAKRFNATAGLATLGGQAMHL
                     RDIFDQFVPGSFVPNDIFSYAPGGGDMRLRKLWLEEMIVKNPTLNGKKISMPLVTAGL
                     TNGLAMVASLFVDPGDTFVLPDLNWDNYELIYHHQYGANIRKFPSFTGEGRFNVPGLL
                     NVLESAGTKKAHLLLNFPNNPTGYSLTETEQDELVEGLVALAEKGMILNVIIDDAYFG
                     LFFEDDIAKESIFAKLCAAHERILAIKSDGATKEAMVWGFRIGFLTYGAKGLTDRQHD
                     ALDKKTLAAIRTSVSNCDRPGQSLLIKAMQEGQRYEIDKQAVFSEMKQRYLVLKETLK
                     RFEGDENLIPYPFNSGYFMAFKTRKSAEELRRHLLDRYEVGTINIADTTLRVAYCSVD
                     LEKVGELIELVYQAAGEIWN"
     gene            1232..1609
                     /locus_tag="PLF67_03990"
     CDS             1232..1609
                     /locus_tag="PLF67_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE89208.1"
                     /translation="MELKSKLYLVNEQGEKFMGIGVLWLLEEIARCSSLRKAALVLGI
                     SYSKAYGMIENLEKGLDVKMLERKRGGSSREGATLTKAAERFIGIYRTFHLEAKERLE
                     APYERLVEAMKSVMEEDDEHKEL"
     gene            1590..2918
                     /locus_tag="PLF67_03995"
     CDS             1590..2918
                     /locus_tag="PLF67_03995"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOE89209.1"
                     /translation="MSTKSYDFTISSLGKAKVPNPIIMGDKHGDVQVDYVRDSDHILF
                     GIEAVMNEVGRQVPRFEETVELAGPREKIFFNPKHVHAAIATCGGICPGLNNVIRSVV
                     RCFWYRYGVRRISGIQYGYQGLLASSPWPIIPLDPDVVDDIQEKGGTILGSARGGGKQ
                     VEEIVDSLERLNVNILVTVGGDGTLRGARDIAEEVGRRGLKIAVIGIPKTIDNDLSLI
                     QSSFGVDTAVQMAVPVVRGAHVEAKNSIHGIGLVKVMGRESGFIAANTSLAQSDVNFC
                     LIPENPFDLEGPNGFLAHLKKRVLDRDHAVVLVSEGAGQDLLPETGEVDASGNPKFHD
                     IGIYLKDEIIKYFKKEGVEINLKYIDPSYIIRSAPADSYDSIYCARLGAHAVHAAMAG
                     KTSLLIGMLHNRFVHLPIDVAVQERNYVDLRGTLWRDVLENTRQPISMKN"
     gene            3234..3944
                     /locus_tag="PLF67_04000"
     CDS             3234..3944
                     /locus_tag="PLF67_04000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE89210.1"
                     /translation="MINDIDTILFDLDGTLLPLDQDRFAQGYFELFAKQSGLLGYDVK
                     AMAKSLQAGLLAMMNNDGSMTNKERFDSVFTSVTGYDAQEMNERFLPFYEGMFESLVE
                     YAAPTPLARTIVDTLASKGYRLVLATNPLFPRVGTLVRMRWVNLFEDDFACVTTYEDF
                     SYSKPNLGYYREIVDRLELDPARCLMVGNDVGEDMVVLEMGMKAYLITDCLINPHNTP
                     LSHFRHGSLKTFATEILS"
     gene            3941..4882
                     /locus_tag="PLF67_04005"
     CDS             3941..4882
                     /locus_tag="PLF67_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE89211.1"
                     /translation="MNDNSSLLTKPVVAALLAIFCTLLWGSAYPAIKIGYELFQVGSD
                     DTAAKMAYAGLRFTLGGLLVLAIRPLMGKSQGGIRTLKKRHWKQIIILGLLQTTIHYT
                     FFYIGLSYTSGSKGAIINASSVFFSALLAHFFHADDKITLRKGLGVVLGFSAVIFVNL
                     DGDLNFSFLLQGEGFIILAALLHSSSGLYSKHIAKEVDPVLLTGLQLAIGGIVLLAIG
                     LLMGADFPTSGFSGYLLMLYLAALSAIAFSIWTSLLKYNKVSSITIYNSLIPVFGAIL
                     SGLFLKESIAGVQLIGAMVAVSLGVVLVTYSSSKTPV"
     gene            complement(4855..6753)
                     /locus_tag="PLF67_04010"
     CDS             complement(4855..6753)
                     /locus_tag="PLF67_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89212.1"
                     /translation="MRGPRMHGGGGGPKGRVPVKLDFGTFKRLLAYIFVPYKVRFVIV
                     VIAILLSSVAGVAGSLFLKVLIDDHITPMVAQGSGASFASLLRAIGVMASIYLVGIVS
                     TLLYNLLMIEIAQGSLKSIRLKMFIHMQSLPISYFDRRSHGDVMSLYTNDVDTLRQMV
                     TQSLPHFVNSAATVSAIFLAMIFSSWQLTLVILLTLSIALRLSTMVTGKSGSYFMKQQ
                     QALGKTNGFIEEMIHGQKVIKVFTYEEKAKARFDELNEELYSSAFNAHRFANILMPMM
                     GNIGYIQYVLVAIVGGLLAIHGTGAITLGTIAVFLQLSRNINRPITQMANQLNSVAMA
                     LAGTKRIFELLDQESEIDEGSIRLVNMCKDNPDSVQVEEQTGCWGWKDGEKITRLRGV
                     VKLEHVDFSYVEGKQVLHDVTLTADAGKKIALVGATGAGKTTITNLINRFYDIDGGTI
                     HYDGIPIRRIRKADLRRSLGVVLQEVNLFSGTVMENIRYGRLDATDEEVIEAAKLANA
                     DYFISHLPEGYQTELRGDGSSLSQGQRQLISIARAIVADPPVLVLDEATSSIDTHTEE
                     IVQKGMDALMEGRTVLVIAHRLSTIHNSDLIIVLENGSIIESGTHDELIARQGQYWRL
                     YTGVFELE"
     gene            complement(6750..8480)
                     /locus_tag="PLF67_04015"
     CDS             complement(6750..8480)
                     /locus_tag="PLF67_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89213.1"
                     /translation="MIPALRKQAVGNRLPTFLTILFVLLEVAMDVTIPYLMANLLDRG
                     ISGASMNEIIFWGAILLLCSFVALAFGILSGHFAARASTGFARNVRKSLYHKIQEFSF
                     ANLDRLPSSSLVTRLTTDVTNVQMAYQMLTRIAVRSPAMLFFSFFMAYTINASLSLVF
                     LVAIPTLALGLFLLIRAAYPIFTKVFKTYDRLNTVVQENIRSIRVVKSFVREEHEIEK
                     FNGVNTEIYKQFSKAEKIIAFNSPLMQGTMYISILVISYIGAKLITSSSMTTGQLMSF
                     ITYTSQILMSLMFLSMVVVMLTISRAAIQRIEEVLKEESDITEKADAVTTVKDGSIIF
                     DDVSFSYTNQADKLNLEGITLDIPSGSTVGILGPTGSGKSSLIQLLARLYDVYSGSVK
                     IGGVDVRDYSIRSLRDQVGVVLQKNTLFSGTVAENLRWGNEDASQEELIRVAKIAQAH
                     PFIDELEEKYDSVVEQGGTNFSGGQRQRLAIARALLKRPKILILDDSTSAVDTKTEAS
                     IRAALKNELKETTKIIIAQRIGSVIDADQIIMLEDGKIHAVGNHETLLGSCARYQEMY
                     ASQMRKEERA"
     gene            complement(8580..10148)
                     /locus_tag="PLF67_04020"
     CDS             complement(8580..10148)
                     /locus_tag="PLF67_04020"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOE89214.1"
                     /translation="MKYINLTEANAFKALESAPQIGSKDLLTKERILSCSVPSAQGVA
                     YNYAAMPVTEEVLDLFQALSDELQLTEKYQILLDGAVMNTGEKRLVLHHLTRGQIGKN
                     VMVDGENKGEFYRKELSKVQTFAKEVRSKSLLLSTGKPVANVVQIGIGGSDLGPRALY
                     LALKEWAEKEGKDHIPASFISNVDPDDPSAVLSALDAEETLFILVSKSGTTLETLTNQ
                     RFVEAWLKKRNPAIDPSKHIVAVTSKTSPLAKSKEVFRSFFFDDYVGGRYSSTSAVGG
                     VILSIAFGFEVFEALLKGAHEADKAALHPHVKENAALLDALIGVYLRNVLHYPTTAIL
                     PYSQGLSRFPAHLQQLDMESNGKQVNRDGEPVAYPTGPIIFGEPGTNGQHSFYQLLHQ
                     GTDIVPLQFIGFSESQRKDDIAVADSTSQEKLVANLVAQIVAFALGKDDDNPNKRFEG
                     NRPSSLLYAKRLTPEVLGTLLAHYENKVMFQGFVWNLNSFDQEGVQLGKVLATKVLQG
                     AAGDEILQAYRSLF"
     gene            complement(10152..10622)
                     /locus_tag="PLF67_04025"
     CDS             complement(10152..10622)
                     /locus_tag="PLF67_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOE89215.1"
                     /translation="MKSRHNRLAVVKELIRNNRIDNQDTLLSMLKVEGFTVTQATLSR
                     DLKMLKVGKISDGWSGYYYSLPENDLVSESEKSYIQDVRRGIISIEFSGNFGVVKTRP
                     GHANSVGIALDILAIPEILGTLAGDDTIFIILREGMTKEDLLESFKTRIPDIEE"
     gene            10773..11324
                     /locus_tag="PLF67_04030"
     CDS             10773..11324
                     /locus_tag="PLF67_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOE89216.1"
                     /translation="MLIVYVLLALVVLLVIWGVSVYNRYVRLRNQAEEAESAIDAHLK
                     QRYDLVPNLVETVKGYAKHEEQTLTKVIEARNKAMSAGTLEEKDQLNASFSASLKTLF
                     ALSEAYPDLKANQGFLDLQAQLSKIETQLLSARKYYNAIIKQLNTICEVFPSSIVASI
                     ARFAKKPYLQIEDEARARVDVKF"
     gene            11324..13189
                     /locus_tag="PLF67_04035"
     CDS             11324..13189
                     /locus_tag="PLF67_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOE89217.1"
                     /translation="MKRRSFLAFLLLLALVSLTAGDYTFRQIDLSVKVGPDNSYDVTE
                     RIVAEFFVPKHGIFREIPVRYGNQRIGLKNLQASDPIIKDDVSSGWATFRLGDANVTI
                     KGLKEYVISYTLEIGDDRNRYGDFFYYNIIGPGWQEEIGLLTFTVEFPHPIDPQKVSL
                     TGGRSGSTLQRGEYMISSDRMTITGSAQKMKPGEALTLYIDLEEGYFNEVKKFIDLTI
                     PLYIGAIILFLLLGFHSFALWKRYGRDEPFVPVVRFEPPTDLSPLEVGYLFDGTVDMK
                     DLVSMLFYWADLGYIRIEELGKKDYRIIKLKNLVSGKEHERRLFNAFFKRGDGTSVDL
                     KDLSKSTTFAKDIEKTKSLTRSHFTGERELKDQNAERKRTVPFLYGVLLVVFHAWGTT
                     YSYPGGVIFTLPLTFGIGLLVLTAVAIPKIFDSWMFARKGKALLKSGAFLLVYLASFG
                     IIYIMYREEVLLSSALSLLFSASALGIPIIFATFGAITAKRSSYGQKILEEIVGYREF
                     IEKVEIDKLKVMIKDDPELFYHVLGYAVVLGLENTWAKKFDSIAMTQASWYIGSGGIH
                     PFFYASMANNLTTGAISKALYTQASSGSGRGPISPSFGGGGFSGGGFSGGGGGAW"
     gene            complement(13243..13521)
                     /locus_tag="PLF67_04040"
     CDS             complement(13243..13521)
                     /locus_tag="PLF67_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOE89218.1"
                     /translation="MAKKIYVGNMSYNTSEAELRELFAQFGTVLSANIIIDRETHRPK
                     GFGFVEMEDNNAAEAAISQLDGKEFGGRNLRVNEAIAKSRPAYGSHRN"
     gene            complement(13736..14143)
                     /locus_tag="PLF67_04045"
     CDS             complement(13736..14143)
                     /locus_tag="PLF67_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89219.1"
                     /translation="MSEERMRILNLLAEGKISAEEAEKLLAASEKAVKAPNVKSAAGK
                     YFYVQVDPKEGKSSEKVMVKVPMALLKAGLNISKLIPSEAQDSIRESMQSKGIAFDFA
                     SLDGENIDEIFEALKEMSIDVETEETTVRVFCD"
     gene            complement(14158..14421)
                     /locus_tag="PLF67_04050"
     CDS             complement(14158..14421)
                     /locus_tag="PLF67_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89220.1"
                     /translation="MPPMLMIFRLKRPERRPISLYIPLLLAYIPLVPLVLLALVSLPF
                     LAISEKTRQYLPLVKALPSLLIATRGTRISVESNDHTIYLYIK"
     gene            complement(14421..14786)
                     /locus_tag="PLF67_04055"
     CDS             complement(14421..14786)
                     /locus_tag="PLF67_04055"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HOE89221.1"
                     /translation="MEHSWQRLLAMTGGKRFLVTEIHIPEEGITIGGSFELPSLARIP
                     IEDQIFIAMFIKTNGSIKQMESIFNISYPTVKNRLNRIAKQLDIGDIEVRTPSRMADL
                     LTRLEEGTITVADALREIE"
     gene            14993..15667
                     /locus_tag="PLF67_04060"
     CDS             14993..15667
                     /locus_tag="PLF67_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89222.1"
                     /translation="MIIPMDSNILGLILSYAFLGVVIAFSIFLKNRFGISSESMRKIV
                     HIGVSNWWFLNLHYFTSLGWAVVGPICFIVVNSLFTLFNWGKHIGLDDRKRNWGLVYF
                     PVTLLLLVVGMYRGVFTPLEATIAVMVMGWGDGLAAMVGSKWGRKKIKTLNKSYPGCL
                     TMGIVSFVVTALALASGSSLSTGMILLASFLVGAVAMFVEAFTPWGLDNITVPLAVLL
                     VLRFFR"
     gene            15664..16539
                     /locus_tag="PLF67_04065"
     CDS             15664..16539
                     /locus_tag="PLF67_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOE89223.1"
                     /translation="MSSWALLVQPTSSLTSLFDRWFFTLDGALWILVGIMGVIALGAF
                     LARQLTFGGALAAFLVGFFPTWILGFGALFSLLFFFIATAILGKISKCLRRVDVEKIH
                     QKTGRRDMVQVFANGLMALLAALLFTYRQNPAYIIMFGAAVGEAASDSFASEVGILSK
                     RPPVSILTGRPMDRGLSGAISALGLVAALMGALLVAIVVSSGYFEVGARAFRAASIIT
                     VAALFGCLLDSVLGITLQAHYWDEVTKRITEKPMINGRPLPLERGVRWIDNDMVNFIA
                     NISSALLALALASFF"
     gene            16643..18271
                     /gene="groL"
                     /locus_tag="PLF67_04070"
     CDS             16643..18271
                     /gene="groL"
                     /locus_tag="PLF67_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270710.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOE89224.1"
                     /translation="MAKQLQFSEEARRSLLNGVEKISRAVMATLGPKGRLVVLDKKFG
                     APNVTKDGVTVAREIELEDPFENMGAQLLKEVATKTNDVAGDGTTTATVLAWAITKEG
                     MKSVAAGINPMGVRRGIDKAVKDAVAEIKKQAKMIKDKEEIAQVASISANNDRTIGDE
                     IANAMEKVGLDGVITVEESKTIETTTDFVEGMQFDRGYLSAYFANNRDTMTALLDDPF
                     ILIYDKKISNMKELLPILEKVAQSGKPLLIIAEDVDGEALATLVVNSVRGILSVVAVK
                     APGFGDRRKAMLEDIAILTGGEVISEELGLKLENADLSQLGRAKSIKVEKENTTIING
                     AGKASDIKDRIAQIKVQIEDTTSDYDREKLQERLAKLAGGVAVLNVGAATEVELKEKK
                     HRVEDALSATRAAIEEGVIPGGGAALIQAAKILEKVDLSKLPEDERAGYNIVRRALEE
                     PIRQIAENAGLDGSLIADKCKNEKPGRGFDAENFVWVDMFEKGIIDPVKVTRSALQNA
                     ASIAALILTTEAAVTDIPEPPAPMPPGGGMEGMM"
     gene            18452..19489
                     /locus_tag="PLF67_04075"
     CDS             18452..19489
                     /locus_tag="PLF67_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89225.1"
                     /translation="MKKLLLTVFLVLMSFTLVFAAGTKEEAAEAAGGSLNAYTTLEEP
                     LAAALFEMFEKETGIHVNFVRLAGGEAVARLEAESANPQVSIWVGGVGLDHITAKTKG
                     LTTPYFSRYAENTPQQYRDPEGYWFGLYVGPLTFVTNLDRAKALGITPPKSWADLLKP
                     EYKGLIRMASPNSSGTAYNVLTTVRYINNGNEDKMMEYLTALDKNIDQYTLSGSAPGK
                     SVAIGEIPIAIGYAHDQVKLKAGGANIEINAPSEGTGYELASMSLVKGGKEAVNAKKL
                     YDWILSSPDAQKKFTEWYVVLVAKGAPKHPDALSIDQIKTVNQDFVWDGDVVNKTRIL
                     DRWTKEIGNKR"
     gene            19521..21761
                     /locus_tag="PLF67_04080"
     CDS             19521..21761
                     /locus_tag="PLF67_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE89226.1"
                     /translation="MNKKRFVQFVIAAMVFIAADIWIYASLMGSFTAYVEEKNYDEVA
                     LFVQTAPEEKSEYAKWLSQVEEIIPGSVAVYLDYRDHEFVPTVGMNAATDALWQQAGK
                     SNAFRQALESSYYLETIKLRDQYQVRYDLLEPERGSTIPTDRTKVHIYFVPVIADNGY
                     EALGTVMILVPSTGILGFTRLLRIFAVGAAIAFVAILWAVMLTRDPVTSFMVLGLMVI
                     VLIFVAYPLLEALRLSFMKNGRFSLETWKLGLSPTYIVALWGSLKLGVATATASTIIG
                     FMIAFLVERTAFKRKRLISILATMPVISPPFSLSLSIILLFGNNGLITTQLLKMKTFS
                     IYGLGGLTVVQTISMFPIAFLTISGVLKQIDSTVEDASLDLSATRWQTFRSITLPLAT
                     PGILSAWMLVFTNSLADFANPQLLAGSLKVLSTEAYMIVTGRMNDLGGGAMLSFLLLL
                     PTLTAFMVQRFWVARKSFVTVTGKPSTTLADLTSKRVRTVLSVVTYLVIGFIMLLYLT
                     IVAGAFVKNWGIDYTFTLSNWKAVVDSWEAIKSTVLLSLIAAPTAGLLSMIAAMLIVR
                     KNFPGKRFLEVLMMTPYAIPGTLVGISYIIAFNKPPLMLVGTAAIIVINYVIRELPVG
                     LENGITALHQIDPSIEEAAHDLGADVPTVFRTIVLPLVRPAFISSMSYTFVRAMTAVS
                     AIIFLTSAKWYHLTVKILSYSENIRFGLASVLCTVLIIIVLGVFGLMQLLVKDEKLTQ
                     KSIAMR"
     gene            21770..22870
                     /locus_tag="PLF67_04085"
     CDS             21770..22870
                     /locus_tag="PLF67_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89227.1"
                     /translation="MEKKSVSVTLNNVTKIFKDPKGKADVLAVDNADFVVEPGELVTL
                     LGPSGCGKTTTLRMIGGFELPTSGKVYIGSDDVTMLPPNRRDTATMFQSYGLFPHMSV
                     FDNVAYGLRLRKTEPDAIRKQVNEYLSLVGLAGYGERAPGRLSGGQQQRVALARSLIV
                     TPSVLLLDEPLSNLDALLREQMRVEIKRIQRSLGITAVYVTHDRVEAMSLSDRVIVMK
                     DGKIRQIGAPDVIYEKPDSRFVAGFVGKAAFFPVAIGERKEGVWHCMLGDKVVHARSA
                     APDIVKNSEGVLMARPESLRLVDKGEGHLDGRVRMNVYLGHSLESYIQTAFGEVLVQV
                     DDPNARTIRKEGEEVSIEFPPDRVRLLGKSEE"
     gene            23033..23404
                     /gene="yajC"
                     /locus_tag="PLF67_04090"
     CDS             23033..23404
                     /gene="yajC"
                     /locus_tag="PLF67_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOE89228.1"
                     /translation="MTTFITFGLIIAIFYFLIIRPQKKREKETKEMLASIKKGDKVVS
                     IGGIHGTVMIVKETTVVIKVDDNTRIEFSRNAISSIVDRKSDAPPTARKKAKKTEEAA
                     VVEAKEVDVVEAEEVEEKSEK"
     gene            23423..25150
                     /gene="secD"
                     /locus_tag="PLF67_04095"
     CDS             23423..25150
                     /gene="secD"
                     /locus_tag="PLF67_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOE89229.1"
                     /translation="MKKRNRLAIVLVVVALSAYFLFPTIKWYGFVPQEVKELATGSNV
                     QIKEYARGQATRELRALKDLVAKNADAPLPSEYSFLKSAAKENYKAMRQPVPKKWTVR
                     ALFAGFYNEQNMFDAIESYHRTRLLELKALGNKALQLGLDLRGGMSILLEADVDSYVA
                     KKGAPASASEISQAVRQDIEILEKRIDQFGVSEPMIRLQGSDQILIEIPGAADPERVN
                     SFLRGKGSLLFKMVDSELSAQLEQYYLANPGEVYTETGALKQPEFLPAGKMAAGYYVS
                     DEYGIDELARFVVIEEEIGLDGIHLESATTATDPITGRPVVNFKLDNLGGDIFYKLTS
                     TNVGKTLAVVMDGKVKAMATINEGIRSSVQISGFTAEEAADLAIVLRTAALPIELTVS
                     SQQAVGATLGEDAIAVGLKAIALGFFLVVILMFAYYGLSGLVADLALLLNMFIMVSFL
                     SAFNFTVTLTSIAGLILTLGMAVDTNVIIFERIKEERRMGKSDSAAIAAGFNKAFWTV
                     MDANITTIIAALVLSQLGSSAVKGFANTLAIGIVSSVFTALFVSHLVFDAAVGDREGK
                     KLSISWRKK"
     gene            25150..26349
                     /gene="secF"
                     /locus_tag="PLF67_04100"
     CDS             25150..26349
                     /gene="secF"
                     /locus_tag="PLF67_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOE89230.1"
                     /translation="MLKTDTVPVIKLRWFTLSLSIILLAAGVLSIVLFGGFNLGVDFE
                     SGLSQRIQIAPTGLEVAYDGDLDTVLSISANALTVEQRGEGGVATKLLRFSEYPTSGD
                     IATALGGIEGISVRVIDGSLKSGNLLSGYGFPATLGNEAIGLNFRTSGTTSIEEFRTA
                     LSDLGNVRVQTVGDSDSRTFQIRLGIKEGSNQASLEAEVADALGKTFGKDGFVILQSD
                     YIGPKFSATLISSSILAISVALALILVYVWIRFRFAFAVSAIVALIHDVLMLLTFISL
                     LRLEFSSITIAALLTIIGYSLNNAIVIFDRIRENTGLNANMGLRANIDRSVRQSLSRT
                     LMSSLTTLLAILPLAIFASGDIQLFAIKMSIGLVSGVYSSNLLAPSLLYWISKAQKNV
                     QIEDKAK"
     gene            26414..27262
                     /gene="ispH"
                     /locus_tag="PLF67_04105"
     CDS             26414..27262
                     /gene="ispH"
                     /locus_tag="PLF67_04105"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOE89231.1"
                     /translation="MKIVLAKTMGYCGGVSRALDLTEEAIAYATEHALPVYSLGKLIH
                     NPQVIASLAERGMSIIEHPEGYEPGVLVVRAHGISDTLRKEFVDAGFLLFDATCPVVR
                     HNLSNIAHWAKTRPILIIGHKGHPEVLAMQGVHVGGKPIKSFLITNAEETSSLIDRPY
                     AVFVQTTFDQVLLEEIRGSLEAFHHVVYVNEICPSSISRRMAALDLALQCDAALVIGG
                     KQSANTKALAELVSNEGKKAYHIEDSTEIPVEAFSYGTLGILAGASTPSSVIEEVVQT
                     LQRSKT"
     gene            27262..27930
                     /locus_tag="PLF67_04110"
     CDS             27262..27930
                     /locus_tag="PLF67_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOE89232.1"
                     /translation="MESKRGIPIPTIKRFPSYLRLLNVFKEEGREMASATMLAEELNL
                     KPIQVRKDISFTGIEGKPKVGFEVDKLINAIISVLGWDNATDAIIIGAGHLGSALARY
                     EGFGSYGLRIVAAFDTAEEKWGTNVGDVPIHPFDHLHRYIEENHVNIAVLTVPADEAQ
                     HVADQLVALGIMAIWNFAPKDIKVPSRVVLQRTDLATSFAVLSAKVKRVMESREFNEE
                     DDSW"
     gene            27924..28382
                     /locus_tag="PLF67_04115"
     CDS             27924..28382
                     /locus_tag="PLF67_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOE89233.1"
                     /translation="MVEKQLQELIAGWDGSLQWRYAHLANAAALLWEETEGINWLGFY
                     LIDKETDKLILGPFQGKAACTEIPIGKGVCGKAAELKKAILVPDVHAFEGHIACDSAS
                     RSELVVPLIDSTGEVVGVLDVDSPMTGRFTEEDRERFERYAKRISSALWS"
     gene            28709..30448
                     /gene="thrS"
                     /locus_tag="PLF67_04120"
     CDS             28709..30448
                     /gene="thrS"
                     /locus_tag="PLF67_04120"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOE89234.1"
                     /translation="MHEHDRLDTLRHSMAHVMAEAVLELFPGTQIAIGPAIENGFYYD
                     FDLPRPLVNEDLETITEKMKAIIAEDLAFEKKVVTRAEARELFKDQVYKLELLDAIEE
                     DEEVSIYSLGGFTDLCRGPHVESTKELRSDAFKLMNIAGAYWRGKETNPMLTRIYGTA
                     WSNPKELRLYMQHLEDVEKRDHRKLGRDLDLFSLHEEAGSGLVYWHPNGARIRQEIEN
                     FWRAEHYANGYEMVYTPHIGKSWLWETSGHLGFYKEGMYPSMEMDNADYYVKPMNCPF
                     HIMIYNNSKRSYRDLPFRWAELGTVYRYEKAGTMHGLLRVRGFTQDDAHLFVTPDQME
                     EEIAEVLRFSLHMLKSFGFSDIAAYLSTRPEKAVGDPKRWDDATEALRRAIEKEGLNY
                     EVDEGGGAFYGPKIDLKIKDAIGREWQLSTVQFDFNEPERFDMTFVDRDGTEKRPYMI
                     HRALLGSLERFFGVLIEHYAGAFPPWLAPEQIKVIPVSDAYDEYAKSVVSELKKAGLR
                     ASGDYSDNRMNAKIRTAQNLKIPYMVILGEREKENNEVSVRYRTGKQENSITLASFIK
                     QVQAAVAAKAQLS"
     gene            30467..31063
                     /gene="pgsA"
                     /locus_tag="PLF67_04125"
     CDS             30467..31063
                     /gene="pgsA"
                     /locus_tag="PLF67_04125"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOE89235.1"
                     /translation="MNLPNKLTVSRLVLTPLFFIAYHLGSWIDPSLSKLASILTIVMW
                     VLIEASDLFDGIIARKRNLVTDLGKVMDPFADTLSRVTYFLCFTGSGIMPLWALALIV
                     WRELSILFVRMLMMGKGKAVAANIFGKAKAVLYAISGGMGILYGALVSWGVETPILGW
                     GYSVMQVVFALAAISSVASFLTYVRAIIASGALSGMSR"
     gene            31060..>31829
                     /locus_tag="PLF67_04130"
     CDS             31060..>31829
                     /locus_tag="PLF67_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOE89236.1"
                     /translation="MKPIRELSIGDASHIRYVLFDIDDTITTEGKLTAQAYQSLWDLS
                     DAGFKVIPITGRPAGWCDLIARQWPVDGVVGENGALAYWEEEGVLKRLYHPNAVKNTH
                     PILSKIKDRVLSEVPEARVAQDQFSRLFDLAIDFAEEKPKLPLETAERIKRICIEEGA
                     TAKVSSIHVNTWMGVYSKLEMSEFFLSKRFGYDDPASVIFFGDSPNDEPMFAYFPLSV
                     GVASIADYRQLIDHLPAFITKERGGAGFHEGVNRLLALR"
BASE COUNT         6982 a         8548 c         9067 g         7232 t
ORIGIN      
        1 tgctctcgga gaccggccga cgaatgtttt tccccaaggg aatcgtcgcc caaagcgccg
       61 aagcgacgga aaaggccaag cgtttcaacg ccacggccgg tttggctact ctgggcggcc
      121 aggcgatgca cctccgcgac atcttcgatc agttcgtccc cggctccttc gtcccgaacg
      181 acatcttctc ctacgccccc ggcggtggcg atatgcgcct gagaaagctt tggctcgagg
      241 agatgatcgt aaagaatccc acgttgaacg gtaagaagat ttccatgccg ctggtgaccg
      301 caggcttgac caacgggctt gcgatggtcg cttccctgtt tgtcgatccg ggcgatacgt
      361 tcgtcctccc cgacctgaac tgggacaact atgagctgat ctaccaccac caatatggag
      421 cgaacatacg aaagtttccc tccttcaccg gggaaggacg gttcaacgtg ccgggtcttc
      481 tgaacgtcct cgaatccgcc gggacgaaga aagcccacct gctgctgaac ttccccaaca
      541 accccaccgg atactccctc accgagacgg aacaggatga gctggtcgaa ggcctcgtgg
      601 ccttggctga aaaagggatg atccttaacg tgatcatcga tgatgcgtac ttcgggctct
      661 tctttgagga tgatatcgcc aaggagtcca ttttcgccaa gctctgcgcg gctcacgagc
      721 ggattctggc gatcaaaagc gacggggcga cgaaggaagc gatggtctgg gggttcagga
      781 tcggctttct cacctatgga gcgaaaggac ttaccgaccg gcagcacgat gcattggaca
      841 aaaagacgct cgccgcgatt cggacctcgg tttccaactg cgatcgtccc gggcagagcc
      901 tgttgatcaa ggcgatgcag gagggacagc ggtatgagat cgacaagcag gcggtcttct
      961 ccgagatgaa gcagcgctat cttgtgctca aggaaacgtt gaagcgcttt gaaggggatg
     1021 agaacttgat cccatatccc ttcaacagcg gatatttcat ggcattcaag acaagaaaaa
     1081 gcgccgagga gctgaggcgc catctcttgg accggtatga agtgggcacg atcaacatcg
     1141 ccgatactac tctacgcgtc gcctattgct cggtggactt ggaaaaagtt ggagagctga
     1201 tagaattggt ctatcaggcg gcaggtgaga tatggaattg aagagcaagt tgtatctggt
     1261 caatgaacag ggcgaaaaat tcatgggcat cggagtattg tggctcctgg aggagatcgc
     1321 ccgttgttcc tcattgcgga aggccgccct cgttcttggc atttcctatt ctaaagcgta
     1381 tggtatgatt gagaaccttg aaaagggact cgatgtgaag atgcttgaac gcaagcgcgg
     1441 cggatcctcg cgggagggcg ccactcttac aaaagccgcc gaacggttta ttggaatcta
     1501 tcgaaccttt catcttgaag ccaaggagcg gctcgaggcg ccgtacgaaa ggcttgtcga
     1561 agcgatgaag agcgtcatgg aggaagatga tgagcacaaa gagctatgat ttcacgattt
     1621 ccagtttagg caaagcgaaa gtccccaacc cgatcatcat gggggacaag cacggggatg
     1681 ttcaggtgga ctatgttcga gacagcgacc acatcctctt cggaatcgaa gcggtgatga
     1741 atgaggttgg ccgtcaagtc ccccgctttg aagaaaccgt ggaacttgca gggccgaggg
     1801 agaagatttt cttcaatcca aagcatgtcc atgcggcgat cgctacctgc ggcggaatct
     1861 gcccgggctt gaacaacgtc atccggtcgg tcgtccgctg cttctggtat cggtatggtg
     1921 tacgtcgaat cagcggaatc caatacgggt atcaaggcct gctcgcctcc agtccctggc
     1981 cgatcatccc gctcgatccc gatgtggtgg atgacatcca ggagaagggt ggaacgatcc
     2041 taggttccgc tcgcggcggc ggaaagcagg tcgaagagat cgttgattcg cttgagcggc
     2101 tcaacgtgaa catcctcgtt accgtcggtg gtgacggaac cctccgcgga gctcgggaca
     2161 tcgccgaaga ggtaggccgg cgcggtctga agatcgctgt catcggcatc cccaagacga
     2221 tcgacaacga cctttcgttg atccaatcct ccttcggggt ggacaccgcc gttcaaatgg
     2281 cggttcccgt cgttcgggga gcgcacgtcg aagcaaaaaa ctcgattcat ggaataggat
     2341 tggtgaaagt gatggggcgg gaaagcggct tcatcgctgc aaacacctcg ctcgcccaaa
     2401 gcgacgtgaa cttctgcctg attccggaga accccttcga tctggaggga ccgaacggtt
     2461 ttctggccca cttgaagaag cgggttcttg accgcgacca cgcggtcgtc ctcgtcagtg
     2521 aaggcgccgg tcaggacctg ctgcccgaaa ccggcgaagt ggatgcttca ggcaatccga
     2581 aattccacga tatcggaatc tacctcaagg acgagatcat caaatacttc aagaaggaag
     2641 gagtcgaaat aaacctgaag tacatcgatc cctcctatat catccgttcc gctccggcgg
     2701 actcctatga ttcgatctac tgcgcccggc tcggggcaca tgcggtccac gcggcgatgg
     2761 cgggaaagac ctccctcctg atcgggatgc tgcacaaccg gttcgtccat ctgccgatcg
     2821 acgtggcggt gcaggagcga aactatgtcg atctccgcgg aacgctctgg cgcgacgtgc
     2881 ttgaaaacac ccggcagccg atttcgatga aaaactagga tgcaataatt tttgcataca
     2941 tgtaaatatt ttgtgaaatt cgggattcta taggtgtaaa gtcctatagg ataagaaaac
     3001 atttatgcaa aatgatgaga aaatgttgaa tatgtacttg attctttttt agagagtcag
     3061 tactgtactt ccaagaaaga gaacgacagc ttgcgaacca accggttcta aagcacatat
     3121 atcggatttt tttctaacct ccttttgttc ccccaaggtt taacctcctt ttccttgggg
     3181 gttttttatg ccccgagttg acccgcattg atgaaagttc gtactattca tccatgatca
     3241 acgacatcga cacgatcctc ttcgacttgg atggaaccct gcttccactg gaccaggatc
     3301 gtttcgccca agggtacttt gagctgtttg ccaaacagag cggcctgctt gggtacgacg
     3361 tgaaggcgat ggcaaaaagt cttcaagccg gcctgctggc gatgatgaac aatgacgggt
     3421 cgatgaccaa caaggagcgc tttgattcgg tgttcacgtc cgtcaccggc tacgacgctc
     3481 aagagatgaa cgaacgcttt cttcccttct atgaggggat gtttgaatcg ttggtcgaat
     3541 atgccgctcc aacaccgctg gcgcgaacga ttgtcgatac cctcgcttcc aagggatatc
     3601 gccttgtctt ggcgacgaac cccctctttc cgagagtggg cacactggtt cggatgcggt
     3661 gggtgaacct cttcgaagat gactttgcct gcgtcaccac ctacgaagat ttctcgtatt
     3721 ccaagccgaa cttggggtat taccgcgaga tcgtcgatcg attggagctc gaccctgctc
     3781 gttgtctgat ggtaggcaac gatgtcggcg aagacatggt tgtcctcgag atgggaatga
     3841 aggcctatct catcaccgat tgtctgatca acccgcacaa caccccgctt tctcattttc
     3901 gccatggaag cctgaaaaca ttcgctacgg agattctttc atgaacgata actcttcact
     3961 cctcaccaaa ccggtcgtcg ccgcactgct ggccatcttc tgcaccctcc tgtggggcag
     4021 tgcctatccc gcgatcaaga tcggatatga gctttttcag gtcggaagcg acgataccgc
     4081 ggcgaagatg gcgtatgccg gactgcgctt taccttgggt ggtctcctcg tcttggcgat
     4141 tcgcccgttg atgggcaaga gccaaggagg aatccgaacc ctcaagaaac gccattggaa
     4201 gcagataatc attcttggcc tgcttcaaac cacgatccac tacaccttct tctatatcgg
     4261 cctctcctac acgagcggct ccaaaggggc gatcatcaac gcttcgtcgg tgttcttcag
     4321 cgccctgctt gcccatttct tccatgccga tgacaagatc accctgcgca aaggcctcgg
     4381 tgtcgtgctg ggattcagcg cggtgatctt cgtgaacctg gatggggatc tcaacttctc
     4441 ctttttgctt cagggggagg ggttcatcat tctggccgct ctcctgcatt cctcaagcgg
     4501 actgtactcg aaacatatcg ccaaggaggt ggatcccgtt cttctcaccg gtctgcagct
     4561 ggccatcgga gggatcgttc tccttgcaat cggtcttctc atgggggcgg atttcccaac
     4621 gagcggtttc agcggatatc tgttgatgct ctatctggct gcgctcagcg ccattgcgtt
     4681 ctccatctgg accagcctgt tgaaatacaa caaggtcagc tcgatcacga tctacaactc
     4741 gttgattccc gtcttcggag cgattctctc gggtctcttc ctgaaggagt cgatcgccgg
     4801 agttcagctg atcggcgcga tggtcgccgt ttcgctgggc gtcgttctgg tcacttactc
     4861 cagctcaaag actcccgtat agaggcgcca gtattgacct tgccgggcga tcagctcgtc
     4921 gtgcgttccc gactcgatga ttgaaccgtt ctccaggacg atgatcaggt cggagttgtg
     4981 gatcgtcgag aggcggtggg cgatgaccag aacggtccgt ccctccatca gcgcgtccat
     5041 tcccttctga acgatctctt cggtgtgggt gtcgatcgag gaggtcgctt catccaacac
     5101 cagaaccggc ggatcggcga cgatcgcccg ggcgatcgag atcagctgcc gttgaccttg
     5161 ggagagggag gaaccatccc ccctcagttc ggtctgatac ccctccggca ggtgggagat
     5221 gaagtaatcg gcgttggcga gcttcgcagc ttcgatgact tcctcgtcgg tggcgtccag
     5281 gcggccatag cggatgttct ccataaccgt tccgctgaag aggttcacct cctgcaggac
     5341 gaccccgagg gagcgcctga gatccgcctt tctgatcctt cggatcggaa tcccgtcata
     5401 gtggatcgtc ccgccgtcga tatcgtagaa acggttgatc aggttggtga tcgtggtctt
     5461 gcccgctccg gttgctccaa cgagggcgat cttctttccc gcatccgccg tcagggtgac
     5521 atcatgaaga acctgtttac cttcgacgta gctgaaatcg acgtgctcaa gtttgaccac
     5581 cccgcgaagg cgggtgatct tctctccgtc cttccacccc cagcatccgg tctgttcctc
     5641 cacctgaacg gagtcggggt tgtctttgca catgttcacc agccggatcg atccttcatc
     5701 gatctcgctc tcctgatcga ggagctcgaa gatccgcttc gttcccgcaa gggccatcgc
     5761 tacgctgttg agctggttgg ccatctgggt gatgggtcgg ttgatgttcc ggctgagctg
     5821 gaggaagacg gcgatcgttc cgagggtgat cgcaccggtt ccgtggattg ccagcaagcc
     5881 tccgacgatg gcgacgagga cgtattggat gtagccgata ttgcccatca tcggcatgag
     5941 aatattcgcg aagcggtggg cattgaaggc ggagctgtag agctcttcgt tcaactcgtc
     6001 gaaccgggcc ttcgcctttt cttcatacgt gaagaccttg atcaccttct gaccgtggat
     6061 catctcttcg atgaatccgt tggttttccc cagcgcctgc tgctgcttca tgaagtagga
     6121 accgctttta cccgtcacca tggtcgagag ccgaagggcg atcgagaggg tgaggaggat
     6181 gacgagcgtc agctgccaag acgagaaaat catcgcaagg aagatcgcgc tgacggtcgc
     6241 cgcgctgttt acgaagtgcg gcaaggattg ggtaaccatc tggcggaggg tatcgacatc
     6301 gttggtgtag aggctcatca catcgccgtg ggagcgccgg tcgaagtagg aaatcggcag
     6361 actctgcatg tggatgaaca tcttcaagcg aatcgacttg agcgagccct gagcgatttc
     6421 aatcatcaac aggttgtaga gcagcgtcga gacgattccg acgaggtaga tggaggccat
     6481 caccccgatc gccctcagga gcgaggcgaa ggaggcgccg ctgccttggg cgaccatcgg
     6541 agtgatatgg tcatcgatga ggactttcag aaagagcgag ccggctactc cggcgacgga
     6601 gctgaggagg atcgcgatca caacaatgac aaaacggacc ttgtacggaa caaagatgta
     6661 ggcgaggagg cgcttgaacg ttccgaaatc cagttttacg gggactcggc ctttgggacc
     6721 gccaccgccg ccatgcatcc ggggacctct catgctcgtt cctccttccg catctggctg
     6781 gcgtacattt cctgatagcg ggcgcacgac ccgaggagcg tctcatggtt tcccaccgca
     6841 tgaatctttc catcctccag catgatgatt tgatcggcgt cgatgaccga gccgatccgc
     6901 tgggcgatga tgatcttcgt cgtttccttc agttcattct tcaacgcggc gcggattgaa
     6961 gcttcggtct tcgtatcgac cgcgctggtc gagtcgtcaa gaatcagaat cttgggacgt
     7021 ttcaacaacg cccgggcgat cgcaagccgt tggcgctgcc ctccggagaa gttcgttccc
     7081 ccctgttcga cgacgctgtc gtatttttcc tccaactcat cgatgaacgg atgggcttgg
     7141 gcgattttcg ccacccggat caactcctcc tgcgaggcgt cctcgtttcc ccaccggagg
     7201 ttttcggcga ccgttccgct gaagagggtg ttcttctgca gaaccactcc gacttgatcc
     7261 cgaaggctcc tgatcgaata gtcgcggaca tcgacccccc cgatcttcac gcttcccgag
     7321 tacacgtcat acaggcgggc aagcagctgg atgagcgagc tctttccgct tcccgtcggc
     7381 cccagaatgc ccaccgtgga accggaggga atgtccagag tgatcccttc gaggttcagc
     7441 ttgtcagctt ggttcgtata gctgaacgag acatcgtcga agatgatcga accgtctttg
     7501 acagttgtga cggcatcggc tttttcagtg atgtcgctct cttctttaag gacctcctcg
     7561 atccgctgga tcgccgcacg gctgatcgtg agcatcacca ccaccatgct gaggaacatc
     7621 agactcatca ggatctgtga ggtgtaggtg atgaagctca tcagctggcc ggtggtcatc
     7681 gacgatgagg tgatgagttt cgctccgata tagctgatca cgagaatcga gatgtacatc
     7741 gttccctgca tcagcgggct gttgaaagcg atgatctttt ccgctttgga gaactgcttg
     7801 tagatttcag tgttcactcc gttgaatttt tcaatctcat gctcttcccg aacgaaggac
     7861 ttcacgaccc ggatggagcg gatgttctcc tgaaccaccg tattcaggcg gtcataggtc
     7921 ttgaacacct tcgtgaagat cgggtaagcg gcgcggatca gcagaaagag gccgagggcg
     7981 agggtcggga tcgcaaccag gaagaccaga ctcagcgagg cgttgatcgt atacgccatg
     8041 aagaagctga agaagagcat cgccggactg cggacggcaa tccgggtaag catctggtag
     8101 gccatctgga cgttggtgac atcggtggtg aggcgggtga ccagactgga ggagggcagg
     8161 cggtccagat tcgcgaagga gaactcctga atcttatgat agagggattt ccgtacgttg
     8221 cgggcgaacc ccgtcgaagc ccgggcggcg aagtgcccgc tgagaattcc gaaggcaagg
     8281 gcgacaaagc tgcaaagaag caggatcgcc ccccagaaga tgatctcgtt catcgatgcg
     8341 ccgctgattc cccgatcaag cagattcgcc atcagatagg ggatcgtgac gtccatggcg
     8401 acctcgagaa ggacgaagag gatggttaaa aaggtcggaa ggcggtttcc caccgcttgt
     8461 tttctcaatg ccggtatcat gtgcttccga tagtaccgaa cttgggtatc atagacaacc
     8521 gaaagaggag gaaaagggat cgaatctcct gaaaaaaaag cccccgaagg ggcttcggat
     8581 cagaaaagac tccgataggc ctggagaatc tcatcccccg cggccccctg aaggactttg
     8641 gtggcgagaa cctttcccag ctggacccct tcctggtcaa aggagttgag gttccagaca
     8701 aagccctgga acatcacctt gttctcgtag tgggccagga gcgttcccag cacctcgggg
     8761 gtgaggcgct tggcatacag caggctggag gggcgattcc cttcaaagcg cttgttgggg
     8821 ttgtcgtcgt cctttccgag ggcgaaggcg acgatctggg cgacgaggtt ggcgacaagc
     8881 ttttcctggc ttgtcgagtc tgcaacagcg atgtcatcct tccgctggct ctcgctgaaa
     8941 ccgatgaact gtagcggcac gatatcggtt ccctggtgaa ggagctgata gaaggagtgc
     9001 tggccgttgg tccccggctc gccgaagatg atcgggccgg tcggataggc gacgggctct
     9061 ccgtcccggt tcacctgttt gccgttggac tccatgtcca gctgctgcaa gtgggcggga
     9121 aagcggctga gcccctggct gtaggggagg atcgccgtcg tcggataatg aaggacgttt
     9181 ctcaggtaca cgccgatcag ggcgtcgagg agggcggcgt tctccttcac atggggatga
     9241 agagcggcct tgtccgcttc gtgcgctccc ttgaggagcg cttcaaagac ctcgaagccg
     9301 aaggcgatcg aaagaatgac tccgccgacc gcgctcgtgg aggagtacct gcctccgacg
     9361 tagtcatcaa agaagaagga gcgaaagacc tccttgctct tcgccaaggg gctggtttta
     9421 ctggtgaccg ccacgatgtg cttggagggg tcgatcgcgg ggttccgttt tttcagccac
     9481 gcttcgacga accgttggtt ggtcaacgtc tccaacgtgg ttccgctctt ggaaaccaga
     9541 atgaaaagcg tctcttcagc gtcgagggcg gaaaggacgg cgctcgggtc gtccggatcg
     9601 acgttcgaga tgaaggaggc gggaatatgg tctttaccct ccttctccgc ccactccttg
     9661 agggcgaggt agagagcccg cggccccaga tcgctgccgc cgattccgat ttgcacgacg
     9721 ttggcgaccg gctttccggt ggaaaggagg agggatttcg agcgaacctc tttggcgaac
     9781 gtttggactt tggaaagctc tttcctgtaa aactctcctt tgttttctcc gtcgaccatg
     9841 acgttctttc cgatctgccc ccgggtgagg tggtgaagga ccagccgctt ctcaccggtg
     9901 ttcatcaccg ccccgtcgag aaggatctgg tacttctcgg tgagctggag ctcatcgctc
     9961 aacgcctgaa agaggtcaag gacctcttcg gtcaccggca tcgcggcgta gttgtaggcg
    10021 acgccttggg cggagggaac ggaacagctc aggattcgtt cctttgtgag caggtcttta
    10081 ctgccgatct gtggagcgct ttcaagcgct ttgaatgcgt ttgcttcagt gagattgatg
    10141 tacttcatgg attattcctc tatatcgggg atgcgggtct tgaaagactc cagcaaatct
    10201 tctttcgtca ttccttcccg aagaatgatg aagatcgtat catcgccggc caaggtcccg
    10261 agaatctcgg gaatggccaa gatatccaat gcgattccga cgctgttggc gtggccggga
    10321 cgggtcttca ccacaccgaa gtttccgctg aactcgatgg agatgatccc tcggcggaca
    10381 tcctgaatgt aacttttttc cgactcgctg accaaatcgt tttcaggcag gctgtagtag
    10441 tatcccgacc agccgtcact gatctttcca accttcagca tcttcaagtc gcgggagagg
    10501 gtcgcctgag ttacggtgaa tccttccact ttcaacatgg aaaggagggt gtcctgattg
    10561 tctatccggt tgtttcgaat gagttctttt acgacagcta agcggttgtg acgagatttc
    10621 atcttctggg cgctccttac gttaagaata tttatacagc ggacgcgaat aaagatacag
    10681 caatcttgat tgacaatcaa ggcggtcacg catcttgaca cgtcctattc atcggcgcaa
    10741 gctaaagatg accatacgtg ttggaggttc ctatgcttat cgtatatgta ctgttggctc
    10801 tcgtcgttct cctcgtcatc tggggagttt ccgtatacaa ccgttacgtt cgcctccgaa
    10861 accaggccga ggaagcggaa agcgcgatcg atgcgcatct gaagcagcgg tatgatctgg
    10921 ttcccaatct ggttgaaacg gtgaaaggct atgcaaaaca tgaggaacag accctcacca
    10981 aagtcattga ggcccgaaac aaggcgatga gcgccggaac gctggaggag aaggaccagc
    11041 tcaacgcatc gttctctgct tcgctgaaga ctctgttcgc tctcagtgaa gcgtatcccg
    11101 acttgaaggc aaaccaggga ttcctggacc ttcaggcaca gctttcgaag atcgagaccc
    11161 agctgctttc cgccagaaag tactacaatg cgatcatcaa gcagctcaac acgatctgcg
    11221 aggtgtttcc ctcttccatc gtcgcttcga ttgcccgctt tgcaaagaag ccgtatctgc
    11281 agatcgagga tgaggcgcgc gcccgcgtag acgtgaagtt ctgatgaaac gaagatcttt
    11341 ccttgccttt ctcctcctgc ttgcgcttgt atcacttaca gccggcgatt atacgttcag
    11401 gcagatcgat ctgagcgtga aggtgggtcc ggacaactcc tatgatgtta cggaacggat
    11461 cgtggcggag ttcttcgttc ccaagcatgg aatctttcgg gagattcccg tccggtacgg
    11521 gaatcagcgg atcgggctga agaatcttca ggcgagcgat ccgatcatca aggacgacgt
    11581 ctcctccggt tgggcgacct tccggcttgg tgacgccaat gttaccataa aaggtctcaa
    11641 agagtatgtg atctcctaca ccttggagat cggggatgac cgcaatcgct acggtgactt
    11701 cttctactac aatatcatcg gtcccggatg gcaggaagag atcggcctcc ttacctttac
    11761 ggttgagttt ccacatccga tcgatcctca gaaggtctcc ttgaccggcg gcaggtccgg
    11821 ctcgaccctt cagcggggtg aatatatgat ctcttccgat cggatgacga tcaccggaag
    11881 cgctcagaag atgaaacccg gggaagcgct gaccctctac atcgatctgg aggagggata
    11941 cttcaacgag gtgaagaagt ttatcgatct gacgattccg ctctacatcg gtgcgatcat
    12001 cctcttcctt ctgctgggat ttcattcctt tgcgctctgg aagcgctacg gtcgggacga
    12061 gcccttcgtg ccggtggttc gcttcgagcc tccgactgac ctttcaccgt tggaggtcgg
    12121 ctacctcttt gacggaacgg tggacatgaa agaccttgtc agcatgctct tctactgggc
    12181 ggatctgggg tacatccgga tcgaggaact ggggaaaaaa gactatcgca tcatcaagct
    12241 caaaaatctt gtttccggca aagaacatga acgaaggctc ttcaatgcat ttttcaaacg
    12301 cggcgacgga acaagcgtcg atctgaagga cctttccaag tcaaccacct tcgccaagga
    12361 tatcgagaag acgaagagtc tgactcgctc ccacttcacc ggggagcggg agctcaagga
    12421 tcagaatgcc gagcgcaagc gaaccgttcc gttcctgtat ggagtcctgc tggtcgtctt
    12481 ccatgcgtgg gggaccacgt acagctatcc cggaggtgtg atcttcacgc ttccgttgac
    12541 gttcgggatc ggcttgctgg ttctcaccgc ggtcgcgatt ccgaagatct tcgattcgtg
    12601 gatgttcgcc cgcaaaggaa aagcgttgtt gaagagcggc gcatttttgc tggtatacct
    12661 cgcttccttc ggcatcatat acatcatgta tcgcgaggaa gtccttctct cttccgctct
    12721 ttcactgctc ttcagcgcaa gcgccctcgg aattccgatc atattcgcca ccttcggagc
    12781 aatcaccgcc aagcgcagca gctacggtca gaagattctg gaggagattg tcggctatcg
    12841 cgagttcatc gagaaggtcg agatcgacaa gctgaaggtg atgatcaaag acgatcccga
    12901 gctcttctac catgtgctcg ggtatgcggt ggtccttggt ctggagaata cgtgggcgaa
    12961 gaagttcgac tcaatcgcca tgacacaggc ctcctggtat atcggctcgg gtggaatcca
    13021 tccgttcttc tacgcttcga tggcgaacaa cctcaccacc ggtgcgattt caaaggcgct
    13081 ctacactcag gcctcttccg gttccggccg gggcccgatc agcccctcct tcggcggagg
    13141 cgggttcagc ggaggcggat tcagcggggg aggaggggga gcctggtaat gaaaaaacgg
    13201 agcctgcggg ctccgtcctc gttgctgaat caatggtatt ggttagttgc ggtgcgatcc
    13261 gtaagccgga cggctcttgg cgatcgcttc gttgacccgt aggttgcggc caccgaattc
    13321 cttgccatcg agctgggaga ttgcagcttc cgccgcattg ttgtcttcca tctcgacaaa
    13381 cccgaaaccc ttggggcggt gcgtctctcg gtcaatgata atgttcgcac tgaggacagt
    13441 gccgaactgg gcgaacagtt cccgaagttc cgcttcggaa gtgttgtaac tcatgttacc
    13501 aacatagatt ttctttgcca tgtactaaag catccttcac aggctgcgcc tgtttgatca
    13561 atattgctta atgtggcgca gttgtgactc ttacctgcca atgggagtga ttcagcaacg
    13621 ccgatcggac aactagggaa aatgaaacga gctgcaacac aaaactaaaa gcatttgaac
    13681 ggtatcacga gggtattgga tagtcaattc ttaatccggc aaagatatcg attgcctagt
    13741 cgcaaaacac cctgactgtc gtctcttcgg tctccacgtc gatgctcatc tccttgagcg
    13801 cttcgaagat ctcatcgatg ttctctccgt caagggaggc gaaatcgaag gcgatccctt
    13861 tggactgcat cgattcccga atggagtcct gcgcttcgga aggaatcagc ttcgagatgt
    13921 tgagccccgc cttcaggagt gccatcggga ctttcaccat caccttttct gaggattttc
    13981 cctctttggg gtcgacttgg acgtagaaat acttgcctgc cgcactcttg acgtttggcg
    14041 ctttgacggc cttttcgctt gcggcgagga gcttctcggc ttcttcggcg gaaatcttgc
    14101 cctcggcaag caggttcaaa attctcattc gttcttcact cattgtggtt ctcctgttta
    14161 tttgatgtat agatagatcg tatgatcgtt tgattcaacg gaaatccggg ttccccgagt
    14221 ggcgatcagc agcgacggca gcgctttgac gaggggcagg tattggcgtg ttttctctga
    14281 aatcgccaga aagggaagac tcaccagtgc aaggaggacg agcggcacaa gcgggatgta
    14341 tgcaagcagc agggggatgt agaggctgat cggtcttctc tccggtcgct tgagtctgaa
    14401 gatcataagc atcggcggca tcactcgatc tccctaagcg catcggcgac cgtgatcgtg
    14461 ccttcctcca gccgggtaag gagatcggcc atgcgggagg gggtccgaac ctcgatgtca
    14521 ccgatatcca actgtttggc gattcggttg agccggttct tcaccgtggg gtagctgata
    14581 ttgaagatcg actccatctg cttgatcgag ccgttcgtct tgatgaacat cgcgatgaag
    14641 atctgatcct cgatcggtat ccgtgcaagc gagggcagtt caaaggatcc cccgatcgtg
    14701 atcccctctt cggggatatg gatttccgta actaggaatc ttttcccccc cgtcattgca
    14761 agaagtcgtt gccaactgtg ttccataggt tcctccgttg cttccaccat atgcaaatga
    14821 attaataaag tcaatagata ttattaaaat aattaataaa attaaaaata ataattaaaa
    14881 taattaagtt ttactggttg gtgcgagatg ggttgcctgc atatcggcgt atttagatgg
    14941 aagattgggg ttttgaactt tgcgcccctt gaaattgttg aagaggatgc gcatgattat
    15001 ccctatggac agcaacattc tcgggctcat cctttcctat gcgtttctcg gggttgtgat
    15061 agcctttagc atatttctta aaaaccgatt cggcatctcg agcgagtcga tgcggaaaat
    15121 cgttcatatc ggggtttcca actggtggtt cctgaatctg cactacttca cgtcgctcgg
    15181 ttgggcggtc gtcggaccga tctgtttcat cgtggtgaac tccctcttta cgctcttcaa
    15241 ctggggaaag catatcggtt tggatgaccg caagcgaaac tggggcctgg tctatttccc
    15301 cgttacgctc ctgttgctgg tggtcggaat gtaccggggc gttttcaccc ctcttgaagc
    15361 cacgatcgcc gtcatggtga tgggatgggg cgacggactt gccgcgatgg tcggctcgaa
    15421 gtggggaaga aaaaagatca agacactcaa taagtcttat ccgggatgtc tgacgatggg
    15481 catcgtcagc ttcgtcgtca ccgcgttggc gcttgcatca ggctcttctc tttctaccgg
    15541 tatgattctc ctcgcctcgt tcctcgtcgg agcggtagcg atgttcgtgg aagcattcac
    15601 cccgtggggg ttggacaaca tcacggttcc cttggcggta ttgcttgtgt tgaggttctt
    15661 ccgatgagta gctgggctct cttggttcaa ccgacttcat cgctcacttc gctcttcgat
    15721 cgatggtttt tcaccctcga cggagcgctg tggattctcg tcggcatcat gggagtcatc
    15781 gctttagggg cctttcttgc ccgacagctg accttcggag gtgctctggc cgcctttctg
    15841 gtcgggttct tccctacatg gatcctgggc ttcggggcgc tcttttcact cctgttcttc
    15901 ttcatcgcca cggcgatatt gggaaagatc tcaaagtgcc taagaagagt cgacgtggaa
    15961 aagattcacc agaagacggg aaggcgggat atggtccagg tcttcgccaa cggcttgatg
    16021 gcccttctgg cggcgctttt gttcacctat cgccaaaacc ccgcctatat catcatgttc
    16081 ggagcggcgg tgggcgaagc ggccagcgac agcttcgcca gcgaggtggg gattctttca
    16141 aagcgccctc cggtttcgat tctcaccggt cggccgatgg accggggcct cagcggggcg
    16201 atcagcgcct tggggttggt cgcggcgctg atgggggcgc ttcttgtcgc catcgtggtg
    16261 agttccggtt attttgaggt gggagcccgg gcgttccggg cggcttcgat catcaccgtg
    16321 gccgccctct tcggctgctt gttggacagt gtgctcggaa tcaccctcca agcccattac
    16381 tgggacgagg tgacaaaacg gatcaccgag aagccgatga tcaacgggcg gcctttgccg
    16441 ttggagcggg gcgttcggtg gatcgacaac gatatggtga acttcatcgc caatatcagc
    16501 tccgctttgc ttgcgcttgc tctggcttct tttttttgag cacttgcttc ttggataatt
    16561 tatttgtatg ttacagagcg gtgggggatt ttccccgccg aatggtatac agaatgatat
    16621 ttcttaagag aggatattca gtatggcaaa acagttgcag ttcagtgaag aggctcgtcg
    16681 ttcactgttg aacggggtgg agaagatctc ccgagccgtt atggctacat tgggccccaa
    16741 aggtcgcctg gtcgtcttgg acaagaagtt cggcgccccc aacgtgacaa aagacggagt
    16801 cacggtcgct cgcgagatcg agcttgagga tcccttcgaa aacatgggcg cccagttgct
    16861 gaaggaagtc gccacgaaga cgaacgatgt cgccggagac ggaacgacca ccgccacggt
    16921 ccttgcttgg gcgatcacca aagaaggaat gaagagcgtt gccgccggca tcaacccgat
    16981 gggggtgcgc cgcggaatcg acaaagcggt caaggatgcg gtcgccgaga tcaagaaaca
    17041 ggcgaagatg atcaaggaca aggaagagat cgctcaggtc gcctcgatct ccgccaacaa
    17101 cgatcggacg atcggggatg agatcgccaa cgcgatggaa aaagtcggtc ttgacggggt
    17161 gatcaccgtc gaagagtcca agacgattga gacgaccacc gactttgtcg aaggaatgca
    17221 gttcgatcgg ggatatctgt ccgcctactt cgcgaacaac cgcgacacga tgaccgccct
    17281 gttggacgac cccttcatcc tgatctacga caagaagatc tccaacatga aggaacttct
    17341 tcccatcctt gaaaaagtcg cccagtcggg caagcccctt ctgatcattg ccgaagatgt
    17401 cgacggagag gcgctggcga ccttggtcgt gaacagcgtc cggggaattc tcagcgtcgt
    17461 cgcagtgaaa gcccccggtt tcggtgatcg aagaaaggcg atgttggaag atatcgcgat
    17521 tctcaccgga ggcgaggtca tcagcgagga gcttggcctg aagctcgaga acgccgatct
    17581 gagccagctc ggccgggcca agtcgatcaa ggttgagaaa gagaacacca cgatcatcaa
    17641 cggtgcggga aaggcgagcg acatcaagga tcggatcgcc cagatcaagg tccagatcga
    17701 ggacaccacc agtgactatg accgggaaaa gctccaggaa cggcttgcga agctcgccgg
    17761 gggcgtcgcc gtgctgaacg tcggagcagc gacggaagtc gagctgaaag agaagaagca
    17821 ccgcgtcgag gatgccttgt cggcaacccg ggccgccatt gaagagggtg tcatccccgg
    17881 tggcggggca gcgctgatcc aagcggcgaa gattcttgaa aaagtcgatc tctccaaact
    17941 gccggaagac gagcgtgccg gatacaacat cgtccgccgc gctcttgaag agccgattcg
    18001 ccagattgcc gaaaacgccg gtcttgacgg atcgctgatc gccgacaagt gcaagaacga
    18061 gaaacccggt cgcgggttcg acgctgagaa cttcgtgtgg gtcgacatgt tcgagaaggg
    18121 aatcatcgat ccggtgaagg taacccgctc cgcactgcag aacgccgcct cgatcgccgc
    18181 cctgattctg accacggaag ccgccgtgac ggacatcccc gagcccccgg ctccgatgcc
    18241 ccccggtggc ggaatggaag gaatgatgta ggttttcgcc gatagataaa agtctgctcc
    18301 gagtcccctg actcagggca gatttttttc ttggttctca tgagggaatg tccttgacaa
    18361 cgcgcaagac catttcctag agtggtactg gacatcgctt tgtagaaagc ggtgaatcac
    18421 cgattttttt cacacggaag gaggaatttc gatgaaaaaa ttgttgctta cagtgttttt
    18481 ggtgctcatg agcttcacgc tcgtgtttgc tgccggaaca aaagaggaag cggccgaggc
    18541 tgccggtggg tcgcttaacg catacaccac attggaggag cctttggcgg ccgctctctt
    18601 tgagatgttt gagaaagaga cggggatcca tgtgaacttc gttcgccttg ccggtggaga
    18661 ggcggtagcc cgcttggaag ccgaaagcgc gaatcctcaa gtctcgattt gggtcggagg
    18721 tgtgggtctt gatcacatca ccgccaaaac gaaggggttg acgaccccgt atttctcgcg
    18781 ctatgccgaa aatacacccc agcaatatcg cgatccggaa ggatattggt tcggactcta
    18841 tgtcggaccg ctcacctttg tgaccaacct ggaccgcgcc aaggcattgg gaatcacccc
    18901 gccgaagagc tgggccgatc tgttgaagcc ggagtataag gggttgatcc gaatggcaag
    18961 cccgaactcc tcgggaaccg cctacaacgt gctgaccacg gtccgctaca tcaacaatgg
    19021 caatgaagac aagatgatgg agtacctgac cgccctcgac aagaacatcg accagtatac
    19081 cttgagcgga tccgcacccg gcaagagcgt cgcgatcggt gagattccaa tcgccatcgg
    19141 ctatgctcac gaccaggtca agctcaaagc cggaggagcc aatatcgaga tcaacgctcc
    19201 cagtgaagga accggatatg agcttgcttc gatgtcgctg gtgaagggcg gcaaggaggc
    19261 ggtcaatgcg aagaaactgt atgactggat cctttcctcc cccgatgctc agaagaaatt
    19321 taccgaatgg tatgtcgttc ttgtcgccaa gggcgcaccg aagcaccccg atgcactttc
    19381 gatcgaccag atcaagacgg tgaatcagga cttcgtctgg gacggagacg tcgtgaacaa
    19441 gacccgcatc ctggatcgtt ggaccaaaga gatcggcaac aaacgctgat gagctctgat
    19501 tcgtacatag ggtttattca gtgaacaaaa aacgctttgt acagtttgtc attgcggcga
    19561 tggttttcat cgccgcagac atttggatct atgcctcatt gatgggttcc ttcactgcat
    19621 atgtggaaga gaagaactac gatgaggttg ctttgtttgt ccagaccgct cccgaagaaa
    19681 aaagcgagta cgcaaaatgg ctctctcaag tcgaagaaat catccccggc agcgtcgctg
    19741 tctatcttga ctaccgggat catgagttcg tcccgacggt cgggatgaac gccgcaaccg
    19801 atgcgttgtg gcagcaggca gggaagagca acgcgttccg gcaggcgttg gagtcctcct
    19861 actatcttga aacgataaag cttcgcgatc agtatcaagt ccggtacgat ctcttggagc
    19921 cggagcgcgg atcgacgatc cctaccgacc ggacgaaggt gcatatctac ttcgttcccg
    19981 tcatcgccga caacgggtat gaagccttgg gtacggtgat gattcttgtt ccctcgaccg
    20041 gtattctcgg ctttacccgg ctcttgagga tttttgcagt cggagcggcg atcgcctttg
    20101 tcgcgattct ctgggcggtg atgctcaccc gcgatccggt aaccagcttc atggttctcg
    20161 gattgatggt aatagtcctt atttttgtcg cctatcccct ccttgaggcg cttcgtctct
    20221 ccttcatgaa gaatgggcga ttcagtctgg agacgtggaa gctcggtctc tctccgacct
    20281 acatcgtagc cctctgggga tcgttgaagc tgggagtggc aaccgccacc gcttcgacaa
    20341 tcatcggctt catgatcgcg ttcctcgtgg agcgaaccgc cttcaagcgg aagcggctga
    20401 tctccattct ggcgacgatg ccggtgatca gtcctccgtt ctccctttcg ctttcaatca
    20461 ttctcctctt cggaaacaac gggttgatca ccacgcagct tttgaagatg aagacgttct
    20521 cgatctacgg actcggcggc ttgacggtcg tccagacgat cagcatgttc ccgatcgcgt
    20581 ttttgacgat cagcggggtg ctgaagcaga tcgactcaac ggtcgaggat gcttcgttgg
    20641 acctcagcgc cacccgttgg cagacgttcc gttcgatcac cctgccgctt gcgacgccgg
    20701 gaattctctc ggcatggatg ctggtcttca ccaacagcct ggcggacttc gccaacccgc
    20761 agctgctggc cggttcgctg aaggtgcttt ccaccgaagc gtatatgatc gtaacgggaa
    20821 gaatgaacga tctcggcggg ggagcgatgc tttccttcct cctgctcttg ccgaccttga
    20881 ccgcattcat ggtgcaacgg ttctgggtcg ctcgaaagag cttcgtcacc gtcaccggca
    20941 aaccctcgac gaccttggcg gatctgacaa gcaagcgggt gcgaacggtt ctttccgtcg
    21001 tcacctatct cgtgatcggc ttcatcatgc tcctgtattt gacgatcgtc gccggagcat
    21061 ttgtcaaaaa ctggggaatc gattatacat ttaccctttc caactggaaa gcggtcgttg
    21121 acagctggga ggcgatcaag tccaccgttc tgctttccct catcgccgct cccacggcgg
    21181 gtctcctctc gatgatcgct gcgatgctga tcgttcggaa gaacttcccc ggcaagcgtt
    21241 tcctggaagt gttgatgatg accccgtatg cgattccggg aaccctcgtc ggcatcagct
    21301 acatcatcgc gttcaacaag ccgcccttga tgctggtcgg aaccgcggcg atcatcgtca
    21361 tcaactacgt gatccgggag cttccggtgg gtttggagaa cggcatcacc gcgctccatc
    21421 agatcgaccc ttcgatcgaa gaggcggccc acgacctcgg tgcggatgtt cccaccgtct
    21481 tcaggacgat cgttcttccc ttggtgagac cggcgttcat ttcgagcatg tcctacacct
    21541 tcgtgcgagc gatgacggcg gtcagcgcga tcatcttcct tacctcggcg aagtggtatc
    21601 acttgacggt gaaaattctc tcctattcgg agaatatccg attcggtttg gccagcgtgt
    21661 tgtgtaccgt cctcatcatc atcgttctcg gtgtgtttgg tctgatgcag ctgctcgtca
    21721 aggatgaaaa gttgactcaa aaaagtatag cgatgcggta ggatcgagta tggaaaaaaa
    21781 gagtgtttca gtaacgttga acaatgttac aaaaattttt aaagacccga aaggcaaggc
    21841 ggatgttctg gccgttgaca acgctgactt cgtcgtcgaa cccggggaac tggtaaccct
    21901 gctcggaccc tcgggatgcg gaaagacgac gacgctgcgc atgatcggag gcttcgagct
    21961 tccgaccagc ggcaaggtgt atatcggcag cgacgatgta acgatgctgc cgcccaaccg
    22021 ccgcgatacc gctacgatgt ttcaaagcta cggcctcttt ccccatatga gcgtcttcga
    22081 caacgtcgcc tacggcctga ggctgagaaa aaccgagccg gatgcgatca ggaagcaggt
    22141 gaatgaatac ctctcgctgg tcggtcttgc cggttacggc gagcgggccc cgggaaggct
    22201 cagcggcggc cagcaacagc gggtcgctct tgcccggtcg ctcatcgtaa ccccctcggt
    22261 cctcctgctt gatgagccgc tttccaacct ggacgccctc ctgcgcgagc agatgcgggt
    22321 ggaaatcaaa cggatccagc ggtccttggg aatcaccgcg gtgtacgtca cccacgaccg
    22381 cgtcgaagcg atgagccttt ccgatcgggt gatcgtgatg aaggacggaa agatccggca
    22441 gatcggagct cccgatgtga tctatgaaaa acccgattcc cgcttcgtcg ccggcttcgt
    22501 cggcaaagcg gcgttcttcc ccgtggcgat cggggagcgg aaagagggag tgtggcactg
    22561 catgttggga gacaaggtcg tgcacgcccg gtcggccgct ccggatatcg tcaagaactc
    22621 cgaaggagta ttgatggccc gaccggagag cctgcggttg gttgataaag gggaggggca
    22681 cctcgacgga agggtgcgga tgaacgtgta tctcggccac agccttgaat catacattca
    22741 gaccgccttc ggcgaggtcc ttgtccaagt ggatgacccc aacgcccgaa cgatccgaaa
    22801 ggaaggggag gaggtctcca ttgagtttcc gccggaccgc gtccgcctcc tcggtaaaag
    22861 cgaagagtag gaaaacttga ctatcgacaa gtgaacatgg tacgttaagc ggtacgtgaa
    22921 aaattgggtg cccacggcac ccgatttcat tgcaaagtga ggaataccca tgctttcatt
    22981 gttaactgcc atgccagctc ccgaggcgtc cgccgccggt ggtggctcga tgatgaccac
    23041 gttcattacg ttcggactca ttattgcgat tttctacttc ctgatcatcc gcccgcagaa
    23101 aaagcgggag aaggaaacga aggagatgct cgcctcgatc aagaaaggag acaaagtcgt
    23161 ctcgatcggt ggaatccacg ggacggtgat gattgtcaag gagaccacgg tggtgatcaa
    23221 agtggatgac aacacccgaa tcgagttctc ccgcaacgcg atcagctcaa tcgtcgatcg
    23281 aaagagcgat gcaccgccga ctgctcgaaa aaaggcgaag aaaacggaag aggctgccgt
    23341 tgtcgaagca aaagaagtcg atgtggtaga agctgaagaa gttgaagaga aatcggaaaa
    23401 gtaaccgctc ctggagataa tcatgaaaaa acggaatcgt ttggcgatcg tcctcgtggt
    23461 cgtcgccttg tcggcatact ttttgttccc gacgattaaa tggtacggct ttgttccgca
    23521 agaagtcaaa gaacttgcga ccggctcgaa tgtgcagatc aaggaatacg cccgcggtca
    23581 ggccacccga gagcttcgtg cgctgaagga tcttgtggca aagaatgcgg atgcacccct
    23641 gccgagtgaa tactctttct tgaagagcgc ggcaaaggaa aactataaag cgatgcgcca
    23701 acccgttccg aaaaaatgga ccgttcgggc gctgtttgcc ggcttctaca atgaacagaa
    23761 catgttcgac gcgatcgaat cgtatcatcg tacccggctt cttgagctca aagccttggg
    23821 aaacaaggct ctccagctcg gtttggacct gcgcggtgga atgagcatcc tcctggaagc
    23881 ggacgtggat tcctacgttg ccaagaaagg agctcccgcc tctgcaagcg agatttccca
    23941 agcggtccgc caggatatcg agattctgga gaaacggatc gaccagttcg gcgtgagcga
    24001 gccgatgatc cggcttcaag gcagtgacca gatcctcatc gagattcccg gcgccgccga
    24061 ccccgaacgg gtgaactcct ttcttcgcgg aaagggatcc ctcctcttca agatggtcga
    24121 cagcgaactc tccgctcagc tggagcaata ctatctggcc aaccccggcg aagtgtatac
    24181 ggaaaccgga gcgttgaagc agccggagtt ccttcccgcc ggcaaaatgg ctgccgggta
    24241 ctacgtcagc gatgagtacg gtatcgatga gcttgcccgt ttcgtcgtca tcgaagagga
    24301 gatcgggctt gacggaatcc accttgaaag cgcgacgacc gccaccgatc cgatcaccgg
    24361 acgtccggtc gtgaatttca agctggacaa cctcggtgga gacatcttct acaagctcac
    24421 ctccaccaac gtcggcaaga ctctggcggt tgttatggat ggaaaggtca aggcgatggc
    24481 gacgatcaat gaagggatcc gctccagcgt ccagatctcc ggcttcaccg ccgaagaggc
    24541 ggctgatctt gcgatcgtcc ttcgaaccgc cgcccttccc atcgagctga ccgtctccag
    24601 ccaacaggcg gtcggagcga cgctcggtga agacgcgatc gccgtcggct tgaaagcgat
    24661 cgctctcggc ttctttcttg tggtcattct aatgttcgcc tactacggcc tcagcggctt
    24721 ggtggcggat ctggcgcttc tgttgaatat gttcatcatg gtaagcttcc tttcggcatt
    24781 caatttcacc gtaacgctga ccagcatcgc cggcttgatc ctcaccctcg gtatggcggt
    24841 cgatacgaac gtcatcatct ttgagcggat caaagaagaa cgccgcatgg gcaagagcga
    24901 cagcgctgca atcgccgccg ggttcaacaa agcgttctgg accgtcatgg acgcgaacat
    24961 cacgacgatc atcgctgcgt tggttctctc tcaactcggc tcttccgcag tcaagggatt
    25021 tgccaacacc ttggcgatcg gtatcgtcag ctcggtgttc accgcgctct tcgtatctca
    25081 ccttgttttt gatgcagcgg tgggtgaccg cgagggtaag aaactctcca tcagttggag
    25141 gaagaaataa tgttaaagac cgatactgtt cctgtcatca aacttcgctg gttcaccttg
    25201 agcctgtcga tcatcctcct tgcagcggga gttctctcca ttgttctttt cggcggcttc
    25261 aatctgggag tcgactttga aagcggattg agccaacgaa tccagattgc accgaccggg
    25321 cttgaggttg cctatgacgg tgatctcgat acggtgcttt caatttcggc aaatgccctt
    25381 accgttgaac aacggggcga gggcggggta gcgacaaaac tgcttcgctt cagcgaatac
    25441 ccgaccagcg gcgatatagc gaccgctttg ggcggcattg aagggatttc cgtccgggta
    25501 atcgacggat cgttgaaatc cggcaatctg ctctccggtt acggctttcc cgcaaccttg
    25561 ggcaatgaag cgatcgggct caacttccgc acctccggta ccacttcaat tgaagaattc
    25621 cgcaccgccc tctccgatct tggaaacgtt cgggtccaaa ccgtcggcga cagtgacagc
    25681 cggacattcc aaatccgcct tggcatcaag gaagggtcaa accaagcttc attggaggcg
    25741 gaggttgcag atgctctcgg aaaaaccttt ggtaaagatg gatttgtcat cctgcaaagc
    25801 gactacatcg gaccgaagtt cagcgcgacg ttgatctcct cctcgatttt ggcgatttcc
    25861 gtggcgcttg cgctcattct ggtgtatgtg tggattcgct tccgcttcgc cttcgcagtc
    25921 tccgcgatcg tggcgctcat tcacgacgtg ttgatgctgc tgaccttcat cagcctgctc
    25981 aggctggaat tctcctccat cacgattgcg gccctgctga cgatcatcgg atactccctg
    26041 aacaacgcga tcgttatttt cgaccgaatc cgggaaaaca cgggcttgaa cgcgaatatg
    26101 ggtcttcggg ccaatatcga ccgctcggtg cggcagtcac tcagtcgaac gctgatgagc
    26161 tccttgacga cgctgctggc gattctccct ctggcgatct tcgccagcgg agatatccag
    26221 ctttttgcaa tcaaaatgag catcggtctg gtttccgggg tgtactcctc caacctcctc
    26281 gcgccttcct tgctgtactg gatcagcaag gcgcagaaga acgttcagat cgaggataaa
    26341 gccaagtagt gcttttgtat tttgtcgagc cgctcccctt atcgagcggc tttttttttg
    26401 acatactcaa tgcatgaaga tcgtacttgc aaagacgatg gggtattgcg gcggggtgag
    26461 ccgggcgttg gaccttaccg aagaagcgat cgcctatgca acggagcacg cccttcccgt
    26521 ctattcgctg ggaaaattga tccacaaccc tcaagtcatc gcttcgttgg cggagcgcgg
    26581 gatgagcatc atcgaacatc ccgaggggta tgaacccggg gtgctggtcg tccgcgctca
    26641 tggaatcagc gatacgttgc ggaaagagtt tgttgatgcc ggctttcttc tcttcgatgc
    26701 gacgtgtccg gtggtgcgcc ataacctttc aaatatcgcc cattgggcga agacccgtcc
    26761 gattctcatc atcggtcata aagggcatcc ggaagtgttg gcgatgcagg gtgtgcatgt
    26821 cggcgggaaa ccgataaaaa gtttcctcat tacgaatgcg gaagagacat caagcttgat
    26881 cgatcgacca tatgcggtgt tcgtccagac cacgttcgac caagtgctgc ttgaagagat
    26941 tcgaggctct cttgaggctt ttcatcatgt tgtgtacgtc aacgagatct gtccttcttc
    27001 aatcagccgc cggatggcgg cacttgacct cgcgctgcaa tgcgacgccg cgctggtgat
    27061 cgggggaaag caaagtgcca ataccaaagc gcttgcggaa ttggtttcaa atgagggaaa
    27121 aaaagcgtat catattgaag acagcacgga aattcccgtt gaagcatttt catacggtac
    27181 gttggggata ctggctggcg cctccacccc ttcgagtgtt attgaggagg tggtgcagac
    27241 tctgcaaagg agcaagacgt aatggaaagc aagcgaggaa ttccaatccc gacgatcaag
    27301 cggtttccct cctatctgcg gctgctgaac gtgttcaaag aggaaggcag ggagatggca
    27361 agtgcgacaa tgcttgccga ggaactgaat ctcaagccga tccaagtccg caaggacatc
    27421 tccttcaccg gcatcgaggg caaaccgaaa gtaggctttg aggtggataa gctgatcaat
    27481 gcgatcatct ccgtcctcgg gtgggacaac gccaccgacg cgatcatcat cggggcgggg
    27541 caccttggtt ccgctctcgc ccgctatgag gggtttggaa gctacggcct caggatcgta
    27601 gccgccttcg ataccgcaga ggagaagtgg ggaaccaatg taggggatgt tccgatccat
    27661 ccattcgatc accttcaccg gtatatcgaa gaaaaccacg tcaatatcgc cgtgctcacc
    27721 gttccggcgg atgaagccca gcacgtagcc gatcaattgg tcgccctcgg catcatggcg
    27781 atctggaact tcgcccccaa agacatcaaa gttccctccc gggtcgttct tcagcgcacc
    27841 gacttggcga cctcattcgc cgttctttcc gccaaggtaa agcgggtgat ggaaagccgc
    27901 gagttcaatg aagaggatga ttcgtggtag aaaagcagtt gcaagagttg attgcgggat
    27961 gggacggttc gcttcaatgg cggtatgccc acctcgccaa tgccgctgcc ctgctttggg
    28021 aggagacgga aggaatcaac tggctggggt tctacctcat cgataaagaa acagacaagc
    28081 tcatcctcgg cccctttcag gggaaggcgg catgcaccga gattccgatc ggaaaggggg
    28141 tgtgcggaaa ggccgccgag ctgaaaaaag cgatccttgt ccccgatgta catgccttcg
    28201 aaggccacat cgcctgcgac agcgcctcgc ggagcgaact cgtcgtcccc ctgatcgatt
    28261 caacgggcga ggtggtcggg gtattggacg tcgacagccc gatgacaggt cgattcacag
    28321 aggaagatcg ggagcgtttt gagcggtatg cgaagaggat ttcatcagca ctctggtctt
    28381 gactaaaacc atacttgttt gcattctttt accaaggcag tgagaaagac gagtagccgg
    28441 gagcgcagct acagagagat ggttcgcggt tgagagacca tcagtcagcg ttcggtgaaa
    28501 accccttttg agccgcgggt cgaacacgaa gataagtagg ccgcgccgtc gcccctgacg
    28561 aaacagggta atgaagcgcc ccctgtaaag aaagggggaa gcaaggtgga accgcggaga
    28621 gtccccggac cttcgtcctt gcccattggc ccatgccggg aaaaggacgc aggtgtgggg
    28681 atgtttcttt tgataggaga tgcggatgat gcatgaacat gaccgattgg acacccttcg
    28741 ccactcgatg gcgcatgtga tggctgaagc cgttctggag ctgtttcccg gaacccagat
    28801 tgcaataggc ccggcgatcg agaatggctt ttactatgat ttcgatcttc cccggccgct
    28861 ggtcaacgaa gatcttgaga cgatcaccga gaagatgaag gcgatcattg ccgaggatct
    28921 cgctttcgag aagaaggtcg tcacccgagc cgaggcgcgg gagctcttca aggaccaggt
    28981 gtataaactc gagctgctcg atgcgatcga ggaggatgag gaagtttcca tctactcgct
    29041 cggcgggttt accgacctgt gccgcggtcc ccacgtcgaa tcgacgaagg aactcagaag
    29101 cgatgcgttc aagctgatga atatcgcagg agcgtattgg cgcggcaagg aaaccaaccc
    29161 gatgctgacc agaatctacg ggacagcgtg gtccaatcct aaggagctgc gcctgtatat
    29221 gcagcatctt gaggatgtgg aaaaacggga tcaccgaaag ctcggccgcg acctggatct
    29281 tttcagtctt catgaggaag cgggttcagg tctggtgtat tggcatccca acggggcgcg
    29341 gattcgacag gagattgaaa acttttggcg agcggaacac tacgccaacg gctatgagat
    29401 ggtctatacc cctcatatcg gcaagtcctg gctgtgggaa accagcggcc acctcggctt
    29461 ctacaaggaa gggatgtacc cttcgatgga gatggacaac gccgactact acgtcaagcc
    29521 gatgaactgt cccttccaca tcatgatcta caacaactcc aagcgctcct atcgggacct
    29581 cccgttccgc tgggcggagc tcgggacggt ctatcgatac gaaaaagcgg gaacaatgca
    29641 cggcctgctc cgagtccgcg ggttcactca ggatgatgcg cacctcttcg tgactcccga
    29701 tcagatggag gaggaaatag cggaagtttt gcgcttctcg ctccacatgc tcaagagctt
    29761 cggcttcagc gatatcgcgg catatctctc gacccgcccc gaaaaagcgg tgggggatcc
    29821 caagcgttgg gacgatgcga ccgaagcgct gaggcgggcg attgaaaaag aaggcctcaa
    29881 ctatgaagtc gatgaaggtg gcggagcatt ctacggaccg aagatcgact tgaagatcaa
    29941 ggacgcgatc gggcgggagt ggcagctgtc gacggttcag ttcgatttca atgagccgga
    30001 gcgcttcgac atgaccttcg tcgatcggga cggaaccgaa aagcggccat acatgatcca
    30061 ccgtgccctg ctcggatcgc tggagcgctt cttcggcgtt ctgatcgaac actacgccgg
    30121 tgcgttcccc ccgtggctgg cgccggagca gatcaaggtc attccggtca gcgacgccta
    30181 tgacgagtac gccaagagcg tcgtttccga attgaagaaa gcgggtttga gagcaagcgg
    30241 cgactattcc gacaaccgga tgaacgccaa aattcgtacc gctcagaacc tcaagatccc
    30301 ctatatggtc atcttgggcg aacgagagaa ggagaataat gaggtttccg tccgctatcg
    30361 gaccggcaag caggaaaact cgatcaccct tgcctcgttc atcaagcagg ttcaagcggc
    30421 ggtcgccgcg aaggcgcaac tttcctgagg aaaaagcgag gttggtatga atcttcccaa
    30481 taaacttacc gtttcccggc tcgttctcac gccgttgttc ttcatcgcct accacttggg
    30541 ctcttggatc gacccgagcc tttccaagct tgcatcgatc ctgacgatcg tgatgtgggt
    30601 cttgatcgaa gcaagcgacc tgtttgacgg gatcatcgcc agaaagcgca atctggtaac
    30661 cgatctgggg aaagtgatgg atccctttgc cgatacgctc tcacgcgtca cctactttct
    30721 ctgtttcacc ggcagcggca tcatgccgct gtgggcgctt gccctgatcg tctggcggga
    30781 gctttccatc ctctttgtcc ggatgctgat gatgggcaaa ggaaaagcgg tggcggcgaa
    30841 catcttcggg aaggcgaaag cggtcctcta tgcgatcagc ggcgggatgg ggattctcta
    30901 cggtgcgctt gtcagctggg gtgttgaaac tccgattctc gggtgggggt attcggtgat
    30961 gcaggtggtg ttcgccctcg ctgccatcag ctccgtagcc tcgttcctca cctatgtccg
    31021 ggcaatcatc gcaagcggag cgctcagcgg gatgagccga tgaagccgat tcgtgaactt
    31081 tcgatcgggg acgcttccca tatcaggtat gtgttgtttg acattgatga cacgatcacc
    31141 acggaaggaa aactcaccgc tcaggcgtat caaagtctct gggatctcag tgatgccggt
    31201 ttcaaggtga ttcccatcac cggtcgaccc gccggctggt gcgatctcat cgcccgccaa
    31261 tggccggtcg acggagtcgt cggagagaat ggcgcgctgg cgtattggga agaggagggc
    31321 gtccttaaac ggctctacca ccccaatgcg gtgaagaaca cccatccaat cctctcaaag
    31381 atcaaagacc gggttctctc cgaggttccc gaggcccgcg tcgctcagga tcaattttcc
    31441 cggctcttcg atctggcgat cgatttcgcc gaagagaagc cgaaacttcc gctggaaaca
    31501 gcggagagga tcaagaggat ctgcatcgaa gagggggcga ccgcgaaggt gagctccatt
    31561 cacgtcaaca cctggatggg cgtctattcc aagttggaga tgagcgaatt ctttctttcc
    31621 aagcgcttcg gatatgatga tccggcgagc gtcatcttct tcggcgactc gccgaatgat
    31681 gaaccgatgt tcgcgtattt ccccttgagc gtcggagtgg cgagcattgc cgattatcgc
    31741 cagctcatcg atcatctgcc cgcattcatc acaaaggagc ggggcggggc cggctttcat
    31801 gagggggtaa accgcctttt ggcgctgcg
//
LOCUS       DAOSLI010000018        31121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1653_length_31121_cov_26.375813, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000018 DAOSLI010000000
VERSION     DAOSLI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 31121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31121
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1653_length_31121_cov_26.375813"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="cysK"
                     /locus_tag="PLF67_04135"
     CDS             complement(<1..908)
                     /gene="cysK"
                     /locus_tag="PLF67_04135"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOE89237.1"
                     /translation="MTAERYDNITQTVGNTPLVRLNRINEGAAVIYAKLEAFNPLSSV
                     KDRAALAMIEGAEKRGELKSDSVIIEPTSGNTGLGLAMVAAVKGYRLIITMPETMSIE
                     RRKLLSALGAELVLTDGALGMKGAIAKANEIAASLEHSYIPSQFENRDNPGIHYKTTG
                     PEIWDATNGAVDLFISAVGTGGTLTGVGRYLKERNPSIRIVAVEPEGSAVLSTGQSGP
                     HRIQGIGAGFVPAVLDTALYDEVHVVSDQDAATAARNAAKMEGILCGISSGAALAAAL
                     EYAKKPEYRDKTIVVVFPDTGERYLST"
     gene            complement(980..1396)
                     /locus_tag="PLF67_04140"
     CDS             complement(980..1396)
                     /locus_tag="PLF67_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOE89238.1"
                     /translation="MKISTRGRYGLRLLVDIAEQATEAPIALSTIAKRQNLSEKYLQQ
                     VALLLARGGVLTSVKGSGGGYSLAKRPEEILMYNVLDLLEGNIAFEEEPPANETAVQQ
                     VIRHHFYRAVDRNVRTAFGELNLADVMKADWFGYEI"
     gene            complement(1393..1800)
                     /locus_tag="PLF67_04145"
     CDS             complement(1393..1800)
                     /locus_tag="PLF67_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOE89239.1"
                     /translation="MKLSTKGRYSLETMVYLATSKDLVSIRTISEATGISGGYLEQLM
                     IPLRQAGLVVSQRGVQGGYAIARKKITCHDVLLASEGEFTPTPCKDCTQTTICKTHQI
                     WALLENAVIDYSKNVYIEDLAGHLLEGQKGGGI"
     gene            complement(1872..3386)
                     /locus_tag="PLF67_04150"
     CDS             complement(1872..3386)
                     /locus_tag="PLF67_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOE89240.1"
                     /translation="MKTIGFDNERYLEEQRHYILERVKESDGKLYLECGGKLLFDYHA
                     SRVLPGFDPNVKMRVFQSLADEIDVIICIHSGDIERRKMRSDFGITYDADVFKMIDDF
                     AKWGLAVNKVVITRYDGEAKSLQFKQVLEQRGITVYTHSYTRGYPTDIDTIVSDEGYG
                     ANSYIETTAPVVLVTGPGPGSGKLGTCLSQMYHDYKMGRKSRYSKFETFPIWNLGIDH
                     PVNIAYESATADLGDKILIDHFHASEYGTLAVSYSRDLEAFPLLRRILLRIMGEEPYR
                     SPTQMGVNRCGFGIIDDEVVRKAGQQEIIRRYYRSSCEYVQGIATKETADRSRAIMEE
                     AKLDPNQRVVVPAAQEALRRGIERGKGVNRIVCAAALELPTGEIATGCNSPLLHASSA
                     LILNAVKILAGIDHEVDLIPPAIVQSVTAMKRDVLKGRGVSLNLDETLICLAMSRAIN
                     EDARKASEELPRLMGCEVHMTHIPSSGDSSGLRKLLLNVTSDPRFPTSNLYNPA"
     gene            complement(3389..3931)
                     /locus_tag="PLF67_04155"
     CDS             complement(3389..3931)
                     /locus_tag="PLF67_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOE89241.1"
                     /translation="MKTVLVLLAQGFEEIEAITPIDLLRRAGAKVTVATLKEDRAAGA
                     HGITVLSDTTLEKLGDRLFDCVVLPGGGEGSENLAASAAVLTKVIQIAQEGVVAAICA
                     APAVVLGKTGLLDGRKVTGYPGTAELVEGLVFEDDGVVVDGNLITAKGPAFAMDFALA
                     IIKKLFGDEKEEQIRLGILR"
     gene            complement(3941..5008)
                     /locus_tag="PLF67_04160"
     CDS             complement(3941..5008)
                     /locus_tag="PLF67_04160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE89242.1"
                     /translation="MANVDLRIQHIEAMASPAEVFARFPLDKDGSKAIASYRQTIAKI
                     IRGEDSRLLAIVGPCSIHDPKAALEYAQKLAVFAKEVEDQLFVVMRTYFEKPRTVGGW
                     KGLILDPDMDGSYDIEGGISLARSLLVEITHMGLPVGCEVLDPIIVQYIDDLISWSSI
                     GARTTESQVHRNLASGLSVPVGFKNSTSGELINAVYAVKSARSNASFIGMDKDGVSKI
                     FRTTGNELGHLILRGGDDGPNYYEDDVEHARDLMEKEGLLPAIVVDCSHANSRKDHHR
                     QKRVLRSIVDQVRWGERSIRGFMLESNLFEGCQRIGEHPADLIYGVSVTDPCIGWDET
                     VRILRRAYGILKDRTQDVPEL"
     gene            complement(5041..6840)
                     /locus_tag="PLF67_04165"
     CDS             complement(5041..6840)
                     /locus_tag="PLF67_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE89243.1"
                     /translation="MKKKKKEPVISKKKIKRMKRSDGLKMKLVDKYTMERVVAGSASR
                     YPKRIALRTYAEEGSELTYSELKRKSDAVSLYFLEQGFVKGDRIALIGESCPNWMLAY
                     FGLTGIGLTAVPVLPEFSPKEILHILQDGGVKAVVVNARHVEKILPFIRKKPEFLIRL
                     EDLFHIPMPISGELKDRNQFLNAPGRDITRRKVDAKARQLLSEHRPKEEDPASLIYTS
                     GTTGMSKAVMLTHRNLVWNADISSDVYFKVKPGYKALSILPVSHVYEFTIGQVLALLC
                     GCEVVYLGRPPAPSSLLPALKEVRPHIIMTVPLLIEKIYRSSVLPTLKANPKLQRYLR
                     FGITRRFIFRIIGRKLIVTMGGRVRFFGIGGAPLDREVEAFLAEAKFPYALGYGLTET
                     SPLISGGKPKNHTVGVIGKIVEHLEVRLEEKDPATGIGEIAVKGPSVMQGYWNNPALN
                     EEAFTPDGFFKTGDLGFIDKKGRLAIKGRTKTMILGSGGENIYPELIEAVINNQDFVT
                     ESLVIPEGGALVALIKLDIEAFAEKMALSVNEAKSEAARYLNKIREEVNKELNVFSRL
                     SKVHLQEDAFERTPTQKIKRFLYLRKKDESSDA"
     gene            complement(6837..7610)
                     /locus_tag="PLF67_04170"
     CDS             complement(6837..7610)
                     /locus_tag="PLF67_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE89244.1"
                     /translation="MLKRAVLGSGSSGNAYYMSDGETSILLDQGFSLVELNRRLDRFG
                     SSASELDAVFVTHLHPDHAKGVGILARSRDIPIHLHTKAIARNAREFAFLGIPQKQVV
                     EVEVGEVIRLGAMRIFCFTTSHDSPGSVGWYIDHPEERVMLLTDLGKTTEEQKELAQR
                     ASILFLEANYDEKMLEEGPYPRILKHRIRSVEGHLSNDDALTFVQESGFAGNELYFVH
                     LSSTNNDHRLLEGQAKGRVSTPFVVCEKNRWYGTIGAAQ"
     gene            complement(7603..7944)
                     /locus_tag="PLF67_04175"
     CDS             complement(7603..7944)
                     /locus_tag="PLF67_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOE89245.1"
                     /translation="MIQIIGTKQCNETKKAIRFCREARIAHHFVDLNERSLSPGEIAS
                     ILTTYRAEELIDTASKVYRKEGYAYRVYDPLEELREKPALHKTPIIRFRGKTHLGYDE
                     AILRAWGASDA"
     gene            complement(7994..9913)
                     /locus_tag="PLF67_04180"
     CDS             complement(7994..9913)
                     /locus_tag="PLF67_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE89246.1"
                     /translation="MYQTIPQLFETIVEKYPSVAVQLSKDEKGTFQPITYRQLHDEVY
                     ALAAALSFYGIKRGDRVGLISDNRKEWLASDLAILSLGAADVPRGQDAMTHEVAFILG
                     ITEAEFCFVENIAQLKKVASLLDQLPHLATIAVLDRKTTKEQVRDLSIKSDITVLLYS
                     ELIAGGNLLLQDSKKRKAIQEAIATGDAEEIATIIFTSGTTGEPKGVMLSHRNFVYQL
                     EAIPKLIKGLEPEQRWLSVLPVWHSFERILQYVAVSNASTIAYSKPLGSILLNDLAVV
                     NPHWMGSVPRIWEAVRSGVFASMKKTGGIKAALFFFFTGVAATYAKNRDLLLGRVATF
                     RKRNRFVDIVRSILPAVLLFPLYKLGNVLVFSKIRAKLGTSFIAGVSGGGSLSESVDF
                     FFDSIGVKLVDGYGLTESAPVVSIRPLQKSIKRTMTGLEGTELRILDDEGQAVPPGEK
                     GVLYVRGPQVMSGYFKRPELTKTVLSEDGWLNTGDLAMWTHSGEFAIVGRAKDTIVLA
                     GGENLEPVPIETKLAESEYIEQAVVLGQDRKYLGALIVLNKARVAEYLKEKNIPYLEE
                     TMTHLGEVKELIGRTINDVISTKSGFKPFEHIARFKLLSKSFEVGKELSAKQELKRFE
                     IDRMYEEEINELFAG"
     gene            10048..11436
                     /gene="asnS"
                     /locus_tag="PLF67_04185"
     CDS             10048..11436
                     /gene="asnS"
                     /locus_tag="PLF67_04185"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOE89247.1"
                     /translation="MKERIVDVLRKDPSDELIAIEGWVRTKRDSKNVCFLEVNDGSSL
                     KGIQIVFDKNTFSDLAALNNVTTGSAVICEGTLVASGGGGQSVELAASALTLVGECPT
                     DTYPLQKKRHTLEYLREIAHLRARTNTFGAVARVRNTLAWATHKFFQDKNFLYVNTPI
                     ISSSDAEGAGEMFRVTTLDLLNVPKTEDGQVDYTKDFFGKQTFLTVSGQLEGETYALA
                     LKNIYTFGPTFRAENSNTKRHLSEFWMIEPEMAFCDIHGNMALAEEYVKYLFSEVLRK
                     NEEDMEFFSRHYEAEMGTTLQKIIETPFIRLTYTDAVKELAKKNDTFEFPVSWGTDLQ
                     SEHEKYLTEVVFKSPVIVTDYPKEIKAFYMKLNDDEKTVRGMDVLVPRLGEIIGGSER
                     EANLEILESRMRALKLNPEDYWWYLDLRRFGSVPHSGFGLGFERAVQYVTGMGNIRDV
                     IPYPRSPKSAEF"
     gene            complement(11444..11995)
                     /locus_tag="PLF67_04190"
     CDS             complement(11444..11995)
                     /locus_tag="PLF67_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE89248.1"
                     /translation="MKHSIGLLYHNTLERELLLSRTNRLPELECSLCPIDDDRFASTD
                     DLLICLCRDLPNALRMKDITKEHRLLIFAPPNLAQVFTYLHDWRCASVSLNANPAKIR
                     AAIANLLQAVDGVMEVQEPVHLTRRERQVLSLILSGQATAEIAKTLGIANATVTTYKK
                     SLYSKSGTNSLSRLLIWAMVRRM"
     gene            complement(12015..12545)
                     /locus_tag="PLF67_04195"
     CDS             complement(12015..12545)
                     /locus_tag="PLF67_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOE89249.1"
                     /translation="MIELYIDADSCPKPLRAIILKAVTNRNLKAFFVADRLLKDVRQA
                     YEEHTALLRSAVEQPEERRMIKSPITMIVVESGADQADRWIVDHAPPDSLAITRDIIL
                     ADMLVKKGITVLDDRGNVFTNENIAERLSLRNAMTTLREYGVFAEKHKPIGPKEIREF
                     SAAFDRELAKALKQKG"
     gene            12749..14395
                     /locus_tag="PLF67_04200"
     CDS             12749..14395
                     /locus_tag="PLF67_04200"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOE89250.1"
                     /translation="MNISVLQEARYGYKPKLPTILTSGACKLVPVFGKKTSSAADSEA
                     IASLFPNTYGAPIVSYAQGENNGAKTVRNVGVILSGGQAPGGHNVISGLFDAMQAANP
                     ENKLYGFKGGPSGILKDDYRIIDAEYLKEFRNTGGFDIIGSGRTKLETVEQFETCAKV
                     CKKHEIGALIIIGGDDSNTNAAVLAEYFLEKNHGIQVLGCPKTIDGDLKNESIEISFG
                     FDTATKTYAELVGNIARDANSAKKYWHFIRLMGRSASHISLEVALQTQPNFCLIGEEI
                     GAKNQSLDDVVDELVRIIAARADDGNHFGVGLIPEGVVEFIPEIKVLIQEINTLLAKE
                     EKAFGAIEDDHEKIRFVTDLLSKASAHVFSSFPEAFRLQLLADRDSHGNVQVSKIETE
                     KLLSSMVEERLNKLGKGAKFQTLHHFFGYEGRCAFPTNFDADYCYSLGYNAFVLVAQN
                     LTGYLSSVTHLAKPASEWQAGGIPITMMMNMEVRHGESKPVIRKALVELDGEPFKYFA
                     SKRDEWARKTAYTYPGAVQYFGPAEVADRTTITLALEQGR"
     gene            14647..15897
                     /locus_tag="PLF67_04205"
     CDS             14647..15897
                     /locus_tag="PLF67_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOE89251.1"
                     /translation="MKESKTLELKENITNTFLKTVSAFANFGDGTILFGVNDEGNVVG
                     IEHAEESCLDIEHKINDSIKPKPDFTLSITNENAIELKVFEGKYKPYVYKGKAYRRSG
                     TSSVEVDMIELRRLALEGSNLFYDQLACAESNLSFSYLEKKLHHVLDISTLDIDILRS
                     LGFYTKDMKYNNAAALFADRNTFPGVDIVRFGETINVILERETIGNTSLLEQYDTALD
                     RYRRNYQYEVIEGSLRQKRELVPEDAFREALANALVHRDYDVHAHIRIAMFKHKIEIS
                     SPGGLPFGVKKEEYLHGTLSVLKNPIIGNLFFRLGLIETLGTGIRRINEAYREHDIKP
                     TYTILDQSITVELPSTTAEYEVTGDESEIIEALQGGAILSSSEIADRTGFSKSKVVRL
                     MKHLEEKRYVSVSGGGRSTKYSLR"
     gene            16007..16468
                     /locus_tag="PLF67_04210"
     CDS             16007..16468
                     /locus_tag="PLF67_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE89252.1"
                     /translation="MTYNKCLYTVYTFITQRTHQRIWGDTVNIQISNASNDPIYVQIK
                     NQIKSAIISGSMAAGEQLPSIRYLAKELRVSVITTKRAYDELEAEGFINSVQGRGSYV
                     SLQNEEFIREEQLRKVEASLLEAIKGAKLAGLSMKDLKEILDTLEGVEDDA"
     gene            16461..17324
                     /locus_tag="PLF67_04215"
     CDS             16461..17324
                     /locus_tag="PLF67_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89253.1"
                     /translation="MHNAIEIRNLTKNYKGFSLGPLDFTIPKGCMVGYIGENGAGKST
                     TLKAILGLIHADSGEIKIFGNSTNEHEPELMNKIGVVFDAINLPADMKVQEVGTFCKL
                     AFTTWAENTFNDYLKRFTLPDDKKVKALSRGMKMKLSLSIALSHQAELLILDEATSGL
                     DPVVRDEILDILLDFLQDENHTVLISSHILSDLEKAADYIAFLHNGKLLFMEEKDLLV
                     ERYALCSLDDETIGHLDPEAIVGRRRHAFGQDLLVIRNLMPKSIKLTNASIEDIMVYI
                     IKGVESESVAL"
     gene            17305..17931
                     /locus_tag="PLF67_04220"
     CDS             17305..17931
                     /locus_tag="PLF67_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOE89254.1"
                     /translation="MKALLYKDLVSSKSTYLLLLIMVGVIAGIAITQGAMIIIPFIFA
                     YIPMILGAISFGYESQSEFPKFLFTTPISRTTYVKSKYCFSVLFGIMAFVSGLAVFGM
                     EYGSLDFALIMAATSFAVPIIFVALQIPFVFEYGVEKEGIIMVATYAVLFITIRLVGK
                     HLNRMMNLVQTMNRINSYLISVAIVAGAICILVVSIHMSCVIVKRKEY"
     gene            complement(17957..18670)
                     /locus_tag="PLF67_04225"
     CDS             complement(17957..18670)
                     /locus_tag="PLF67_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE89255.1"
                     /translation="MQLLPDIYQLSGSLAGLGGDQILGPWDECNSYLVDGGSSYILID
                     AGNGENIGLIFKRMEMWGLDPDKISTLFLTHAHWDHAGGARLFKDRGVEILSSKETAD
                     SVASADHRCAGFLYHREMTPVTVDRIVGDGEMVSIDALEFEAIHLPGHTLGCMAYLLT
                     FEDRRILFSGDVIGTLGWGDFGWDGSVDFDKQRYIASLFKLQGIPFDVLLPGHGMGSY
                     TDPTKRVDISLNQALIQWR"
     gene            complement(18680..19300)
                     /locus_tag="PLF67_04230"
     CDS             complement(18680..19300)
                     /locus_tag="PLF67_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOE89256.1"
                     /translation="MLDVYERYPQLEGIRKEIEAAADLLVKAAKKGKMILVAGNGGSA
                     ADSEHIVGELMKSFIRQRPIERIVAETLIEVDQEHGSYLAMKLQSPIKAIALPTHASL
                     SSAFANDVDPNLTFAQLTLGWGEEGGVFWGISTSGNAKNVNLAASVAKAKGMKVLAFT
                     GSDGGELAKKADVAIRVGESETYKVQELHLPIYHYLCIRLEEALFS"
     gene            complement(19293..20204)
                     /locus_tag="PLF67_04235"
     CDS             complement(19293..20204)
                     /locus_tag="PLF67_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOE89257.1"
                     /translation="MMFVGLDIGGTKTAVSLGSGDGSIRRKQSFPTRGKPEEVLERCA
                     LIIEAYKKEDDAIRAIGISCGGPLDSEKGIIQSPPNLPLWDDIEIVKYFEERFGIPTF
                     LENDANACALAEWYWGAGVGSSSMIFLTFGTGLGAGLILDSRLYRGVNGLAGEAGHIR
                     IADDGPMCYYKKGSWESYCSGCGISGLYKIATGQERSAKEVCALADANDPAAKKVLEE
                     SAAKLGLGIALLIDLLNVERIIIGSIFERSEHLFRSIMEEVIEKEALQTSRESCTILP
                     SGLGDRLGDMAALGVARDGYQRSTHNA"
     gene            complement(20208..21032)
                     /locus_tag="PLF67_04240"
     CDS             complement(20208..21032)
                     /locus_tag="PLF67_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89258.1"
                     /translation="MKLSKTNRAVKYILAVIAAFLFLFPLYVIINNSLKPFADVRVSE
                     MWVLARSPSSEGFSKAFKALRQNLINSFILVIPVSLLSVLFGSIIGFIFAKVRFKYSK
                     VLFFLVIFGMFIPYQSILIPLVRTLNRVGLYGKLEGLIVTHTIYGLPISSLMFRNYYA
                     KIPDELVEAGMIDGLQLVGVFRQVVVPISLPTMVVVLIWQFTSVWNDFLFAVTLTQKP
                     SIQPVTVALQNLAGTQVIEWNAQMAGALIAAVPTLFVYIFLGKYFIQGLLSGSVKG"
     gene            complement(21035..21922)
                     /locus_tag="PLF67_04245"
     CDS             complement(21035..21922)
                     /locus_tag="PLF67_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE89259.1"
                     /translation="MYCSQKDKRLAIIMLIPFLAVLFLFVYGFIFWSVRVSFSAWDSI
                     LPDMTWVGLRNYQELMGSLRFKTDIVNTIYFTIFFIAICVVGGFILSYLLFVNLKGEG
                     FFRTVYLFPLSLSFVVTGVLWRWIFNPEVGLNALLRTMGFKAEFGWFTSTYSIAGFNV
                     ALIALIIGASWQYLGYTMAMFLAGLRGIDDQLIESAQIDGAGEWAIITRIILPIIKPI
                     TFSAMIVLGHISLKIFDLAYAMTGKGPRFVTDFPGLFMFETTFRGNHYAEGAAISIVM
                     LLLVAIVIVPYLASSLRED"
     gene            complement(21993..23255)
                     /locus_tag="PLF67_04250"
     CDS             complement(21993..23255)
                     /locus_tag="PLF67_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89260.1"
                     /translation="MKKNVLAILVVLLIASALVFAAGTKEQKGANTIEIFSWWTAGGE
                     ADGLEAMFKEFSKKHPQLEIINATVAGGAGTNAKAVLATRMQGGNPPDTFQVHAGHEL
                     IDTWVTTGFMEPVTFILKDAGFMDKFPADVIDIISYKGEVYSIPVNIHRSNVMWYSPK
                     LFAKFNLKPPKTMDEFFRVADVFEKNGIPALALGDNESWAATHLLESVILASLGPDRY
                     RDLWNGKVKWNDPKVQEALVNYTKMMKYTNRDHAALANMDAAGYVAEGKAAMNVMGDW
                     AAGYYYSQNFKQGEDWDWAPAPGTGGVFIMLSDSFGLPKNAPNRENVVKWLELCASKE
                     GQDAFNPIKGSIPARTDGDRSKYDRYLNAAMDDFASNAIVASVAHGAAISEPWLTRVN
                     DIMAAFTTDLDVAKATREFQAAADRYAP"
     gene            complement(23298..24569)
                     /locus_tag="PLF67_04255"
     CDS             complement(23298..24569)
                     /locus_tag="PLF67_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOE89261.1"
                     /translation="MKKKFTAWKDELQHHLDKELLPFWLDRLWDEEHGGFLTQWDKDG
                     KDSGVDEKSMLAHFRSIYSLSLAALHGHDHDGRCQSLATKGVHFAIEHYWDEKYGGFY
                     WLFDRKNNVLIDQKITYGHSFVIYALSTYTLAFGDPIGLEYAERVFDLLQVYVVDTNY
                     GGYWTMLNRNWSLCGPGSAGGDRKTLDIHMHLMEAFTALYQASGKEIHRRKLLEIITQ
                     IDEKILHPEFKTGIPMFWEDWTIAPHIKFDIIWGWDRFEGEGGEKEHALDNTSYGHNA
                     ELFWLLAEALKSADVPLKAWEPTFRKMLDHSVAYGVDEKYGGVYVEGSLTTSAVHDKA
                     KEFWQQAEFMIGMLDAYLLYGDERYIKAYENVHRFVFDVMINHDVGEWLPLLQQDGTP
                     IWRHMSHSWKVNYHTIRAVVLSINRLERLME"
     gene            complement(24576..25640)
                     /locus_tag="PLF67_04260"
     CDS             complement(24576..25640)
                     /locus_tag="PLF67_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89262.1"
                     /translation="MTYYPLRERKNKVYIEDATILPENDSFPLADEQEVWIDALCDEI
                     LEARRNKHPVILAFGAHAIKNGLGQLLGRFAEEGYFTHLATNGAGIIHDWEFAYQGRS
                     SEDVKANVAEGKFGTWEETGFSLNLALAVGAFEGCGYGESIGKMIEEDGVMIPTQEEL
                     LETSLDQSASFEKRSGALDLLDLIVKLDLQPGWLGIDHPYKEYSLQHCAYVSDVRFTG
                     HPMFGHDIIYTHKANRGSAIGRTAERDFLVFVDSIARLEGGVYLSVGSAVMSPMIFEK
                     ALSMVRNTGVRIDHAVIRVVDLQKGTWDWNRGEPPEDNPAYYQRFMKTFSRMGLESHY
                     LCIDNRSLFVNLYTALKRKG"
     gene            complement(25651..27552)
                     /locus_tag="PLF67_04265"
     CDS             complement(25651..27552)
                     /locus_tag="PLF67_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOE89263.1"
                     /translation="MRKADALKTFLSPSKPVALAVIGDLCLDLAYQVTTEGGEISVET
                     GLQTYSVQSVKGELGGGCNVGLNLKTLGARRVDMYGIVGTDLYGKVLCDLLESHNLTI
                     EGVQTQGEGWATHVYHKVFEQGIEHPRYDSGNFNVPGERSIDLLLETLRKKIDSYDAI
                     IINEQVPQGLHSKVFQERLNRILDEYGEKVLWFADCRKLNSVYRNAIHKVNEAEGRAL
                     SPGFASLSTKELVKALYGYFGKPTVVTLGENGAVGFDGDVFSSVLGVHFTQHIDIVGA
                     GDAFLAAMALSMSRGASLAEGIEVGNLSAAQSLLTLYGCGHPTVEEILTLKEKAAWRY
                     NPEKAADDANRVFDSGGTIEIIDPVSKRGLPKIAIFDHDGTISTMRSGWEGVMAKMMK
                     EAIAGSALSTLTNEEILKLEAEIAHFIDQTTGIQTIEQMVYLTEMVARYGYVPSDEIL
                     SAQEYKNIYNDDLLAMIASKKAGVANGTIPPSEVTIAGSVEFLHYLYERGVKLYLASG
                     TDQADVIREAALLGYADLFEGRIYGSVGKIDEDPKKVVLRRLITSLAGQEGDIIVFGD
                     GPVEMREARSRGVLAVGLLSDEVRRSGENLAKRQRLVLGGASLLMPDFASSKELMNLL
                     GWNGSEQYV"
     gene            27646..28635
                     /locus_tag="PLF67_04270"
     CDS             27646..28635
                     /locus_tag="PLF67_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE89264.1"
                     /translation="MKHKTISDIAREAGVSKATVSRVLTHPELVKPATQERVKRVMEK
                     HEYVPNHIAQSLAGNPTKMIGVVIDELSNFFFIEVAEGISNILSPLGYSMLLVSSRWI
                     EEEETRLVKSLISSQVDGVLLAPVAKESKALHLLKASQTPCVIINQIPSDASLSFVAG
                     DDLEGGKIAAALVNEQEAEQVIVITGFAHQSIEDRVAGFKKVLDPRFRYKRYRFVETQ
                     ADGAALVAALIDKEALDKKKTILFVTNDNVAIGIVNELVEQGISIPEQVKVIGYDNIR
                     ATSFCRIPLTTVSQSTIHIGEQAAKALLQLINKERSEPLHQLIEPVLVRRHSL"
     gene            28710..31109
                     /locus_tag="PLF67_04275"
     CDS             28710..31109
                     /locus_tag="PLF67_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOE89265.1"
                     /translation="MHTIDMNGIWKLSPRDRHAIAPHCPSFLEIPSIDCTLPGDVHSA
                     LLKNGLIPDPFVGTNELEIQWVGRHDWILERTFTLTEEDFACGTPFITFTMADTMITL
                     FINDRQVGTMYNQFRRWRFEIGEHLQVGENTIKLIFESAENAAIRESRQIPYSEYPVH
                     AKHRNLIRKTQCHSGRDWGPCIMTFGIYEPVKIEFVDEGFIDSVKFTTVETGASSFDV
                     TVEVEYAPLEAIEALDFTATVAGSSQTVTQTFSPGRSNTASLTFAVSDVRRWRPSGEG
                     KAVLYPLTVTAGSQSVTRLVGFRTLEVETIEDGEGGKSMTFIVNGERIFAKGANWIPI
                     DSFPSRMTGERYEHLLQSMVDANMNMVRLWGGGMYEHKWFYDLCDEKGILIWHDMMFS
                     CAIYPATDPFLSNVEEEIRYQVNRLQAHPSIALWCGNNEDVVGKTVKAADPSRRWWPS
                     SPSRSEGEHIDNRGDMHIPSVWHDGSPFERYYSYAPRFVSEFGFQSFPSLSTVNRYAD
                     EEMHNLTSSVMEHHQKHPRGNSIIIEYFTQYYRLPTSFEQLLYLSQVQQARAMKIAIE
                     YWRTLYGRTMGTLYRQLNDIWPGASWSSIDYYGKWKLLHYAAKRFFNPIHPIVYMKED
                     GIVEVYVVNDSAREIADEAKLSVKYSTYEGNKLGKSEYPVVIKPRSSIHVTTIDLSRK
                     SRIDRSNTFIYVKLKSDELYMENTLLLAKPKASKLRNPEIKTTVANAPGGFSVTVSCR
                     YPAFEVALDSGSIAGIFSDNLFDIRPTAQKVIFFKSAKKVSLEAFKAELKVFDLYSSS
                     R"
BASE COUNT         7037 a         8732 c         8310 g         7042 t
ORIGIN      
        1 gtggagagat accgttcacc ggtatcggga aagacaacga cgatcgtctt gtccctgtat
       61 tccggtttct tcgcatattc caaggctgcg gccaaagctg ctccgctgga aatgccgcag
      121 aggatgcctt ccatctttgc cgcatttctt gcagcggtgg cggcgtcctg atcgctcacc
      181 acatgcacct catcatacaa cgcggtatcg aggacagccg gaacgaatcc cgctccgata
      241 ccttggatcc tatgaggacc gctctgtccg gtggaaagaa ccgcactgcc ctccggttcg
      301 acggcgacga tcctgattga cgggtttcgt tccttcagat agcggccgac cccggtgagc
      361 gttccgccgg taccgacagc gctgatgaag agatcgaccg ctccgtttgt ggcatcccag
      421 atctccggtc ccgtcgtctt atagtgaatg cccgggttgt cccggttttc aaactgggac
      481 gggatgtagg agtgttccag ggaagcggcg atctcattgg ctttggcgat cgcccccttc
      541 attcccaagg ctccgtcggt cagaacaagc tccgctccga gcgccgataa gagctttctc
      601 cgctcgatgc tcatcgtttc cggcatcgtg atgatgagtc gatacccttt caccgctgcg
      661 accatcgcca gccccaagcc ggtgttgccg ctggtcggtt cgatgatcac gctgtcgctt
      721 ttcaattcgc ctcgtttctc cgccccttcg atcatcgcca gggcggctcg gtctttgacg
      781 cttgaaaggg ggttgaacgc ctcaagcttc gcatagatga ccgccgctcc ttcattgatg
      841 cggttgagtc gaaccagcgg ggtgttgccc accgtttgcg tgatattgtc gtatcgttca
      901 gcggtcatga aaacctccct taatatccta gtattaacct gggaataaca gtatatagaa
      961 gagtaagtga cgtcaacgtc taaatttcgt agccaaacca atcagctttc atcacatcgg
     1021 ccaagttcag ctcgccaaag gcggttcgga cgttgcgatc gaccgctcga tagaaatgat
     1081 ggcgaatcac ctgctgaacc gcggtctcgt tcgccggcgg ctcctcttca aaagcgatgt
     1141 tcccttcaag caggtcaagc acattgtaca tgagaatctc ttccggacgc tttgcaagcg
     1201 aatatccgcc tcccgatcct ttcaccgagg taagaactcc tccccgagca agaagaaggg
     1261 cgacttgctg gagatatttt tcactgagat tctgccgttt ggcgatcgtc gagagagcta
     1321 tcggagcttc ggtggcttgc tcggctatat ccacgagaag acgaagacca taccgcccgc
     1381 gggttgaaat tttcaaattc cccctccttt ctgaccttcg aggagatgcc cggcaagatc
     1441 ttcaatatat acattcttgg aataatcgat caccgcattt tccaacagcg cccagatctg
     1501 atgggtttta cagattgtcg tctgggtaca atccttacag ggcgtcggag tgaactcccc
     1561 ctcgctggca agcagaacat catggcaggt gatctttttc cgagcgatcg cataaccgcc
     1621 ttggactccc cgctgggaaa ctaccaatcc cgcttggcga agcggtatca tcaactgctc
     1681 cagatacccg ccggaaatcc ctgtcgcttc actgatcgta cggattgaaa caagatcttt
     1741 tgacgtggcg aggtagacca tcgtctcaag tgagtatctg cctttcgttg aaagtttcat
     1801 atctcaaagg tatcaacacc ccatcaatgg gtcaagtatt tgacggtttc gcggtgtttt
     1861 cactcgattc gctatgcggg gttgtagaga ttcgaagtcg gaaagcgcgg atcgctcgtc
     1921 acgttcaaca ggagtttcct gaggcccgaa gagtcgccgg atgagggaat gtgggtcata
     1981 tggacttcac agcccatgag ccgtggcagc tcctcgctcg ccttccgggc atcttcattg
     2041 atcgcccgac tcatcgccag acagatcagc gtctcatcga gatttagact gactcccctg
     2101 cctttgagaa catcccgctt catcgcggtg acggattgaa cgatcgccgg cggaatcaga
     2161 tcaacttcat gatcgatacc ggcaaggatc ttcaccgcgt tgagaatcaa cgcgcttgaa
     2221 gcgtggagca gcggggagtt gcatcccgtg gcgatctcgc cggtgggcag ctccaacgcg
     2281 gcggcgcaca cgatgcggtt cacccctttg ccccgctcga ttccccgcct cagggcttct
     2341 tgcgcggcgg gtacgacgac ccgctggttg ggatccaact tggcctcttc catgatcgcc
     2401 cggcttcgat cggcggtctc ttttgtcgcg atcccctgaa catactcaca gctgcttcga
     2461 taatagcggc ggatgatctc ctgctgtccc gccttgcgga ccacctcgtc atcaatgatt
     2521 ccaaaaccgc agcgattcac ccccatctgc gtcggactcc ggtacggctc ttcgcccatg
     2581 atcctgagga gaatcctcct gagcagcgga aacgcctcca gatcccggct gtagctgacc
     2641 gcaagcgttc cgtactccga agcgtggaag tggtcgatca ggatcttgtc ccccagatcc
     2701 gccgtcgcgc tttcataggc gatattcacc ggatggtcga ttccgaggtt ccaaatggga
     2761 aacgtttcaa acttcgaata gcggctcttt ctccccattt tgtaatcgtg gtacatctgg
     2821 ctgaggcagg tgcccagttt ccccgatccc gggccgggcc cggtgaccaa caccacggga
     2881 gccgtcgtct cgatatagga gtttgctcca tatccttcat cgcttacgat cgtatcgatg
     2941 tccgtgggat agccgcgggt gtaggagtgg gtatagacgg tgatcccccg ttgctccaga
     3001 acctgtttga actggaggga ttttgcttcc ccgtcatacc gggtgatcac caccttgttg
     3061 acggcaaggc cccacttggc gaaatcgtcg atcatcttga acacgtcggc gtcataggtg
     3121 attccgaagt cgctgcgcat cttgcgccgt tcgatatcgc cgctgtggat gcagatgatc
     3181 acatcgatct catcggcaag cgattggaag acccgcatct tcacgttggg atcgaagccg
     3241 ggaagaacgc gggaggcgtg gtagtcgaag agcagcttgc ccccgcactc cagatagagc
     3301 ttgccgtcgc tttccttgac gcgctcgagg atatagtggc gctgctcctc cagataccgt
     3361 tcattgtcga agccgatggt tttcacgatt acctcaggat gcctaagcgg atctgttctt
     3421 ctttctcgtc gccgaagagt ttcttgatga tcgcaagagc gaagtccatg gcgaacgccg
     3481 gacccttggc ggtgatgagg ttgccgtcaa cgaccacacc gtcatcctcg aacacgaggc
     3541 cttcgaccag ctcggcggtt ccgggatatc cggtaacctt cctgccgtcc aagaggccgg
     3601 tctttcccaa gaccaccgcc ggggcggcgc agatcgccgc gacgaccccc tcctgggcga
     3661 tttggatcac cttcgtaagg acggcggcgg aagctgcgag gttttccgag ccctctcctc
     3721 cgccgggcag gacgacgcag tcaaagagcc ggtctcccaa cttctccagg gtcgtgtcgc
     3781 tgagaaccgt gatgccgtgc gctccggcag cccgatcctc cttaagcgtc gcgacggtca
     3841 ccttcgcacc agcccgcctc agcaggtcga tcggggtgat cgcctcgatc tcttcaaagc
     3901 cctgagccaa aagaaccaat accgttttca tctcttcctc ctataattcc ggcacgtcct
     3961 gagtgcgatc tttgaggatt ccataggcgc gccgcaagat tcgcacggtt tcatcccagc
     4021 cgatgcaggg atcggtgacg gagaccccgt agatgagatc ggcggggtgt tcgccgatgc
     4081 gctggcatcc ctcaaagaga ttcgattcga gcatgaagcc ccgaatggat cgctcgcccc
     4141 accgaacctg atcgacgatc gagcgaagaa cccgtttttg acgatgatga tccttacggc
     4201 tgttggcgtg ggagcaatcc accacgatcg ccggcaggag cccctccttc tccatcagat
     4261 cccgggcgtg ctcgacatca tcctcgtagt agttcggccc gtcatcccct ccgcgaagga
     4321 tgagatgccc cagttcatta ccggtcgttc tgaaaatttt ggaaacgcca tccttgtcca
     4381 tcccgatgaa agaggcgttt gagcgggcgc tctttaccgc atagaccgcg ttgatcagtt
     4441 cgccgctggt cgaattcttg aatccgacgg gaacgctcag tccgcttgcc aggtttcggt
     4501 gcacctggct ttccgtcgtc cgcgcaccga tcgacgacca gctgatcaga tcgtcgatat
     4561 actgaacgat gatcgggtcg agcacctcgc agccgacggg aagacccatg tgggtgattt
     4621 ccaccagcag cgagcgggcc aggctgatcc ccccttcgat gtcgtaggat ccgtccatgt
     4681 cgggatcgag gatcagccct ttccaccctc cgacggttcg gggcttctca aaataggtcc
     4741 tcatcacgac gaacagctgg tcttccacct ctttggcgaa gacggcgagc ttctgtgcat
     4801 actccagcgc cgccttcgga tcgtggatcg aacaggggcc gacgattgcc agcagcctcg
     4861 aatcttcacc gcggatgatc tttgcgatcg tctgtcgata cgaggcaatc gccttgctgc
     4921 cgtctttatc aaggggaaag cgggcgaaca cctcggcagg actcgccatc gcctcaatgt
     4981 gttgaattcg aaggtctacg ttagccatac gcttggtata gcatatccgc tgctttctcc
     5041 tcaagcatcg gaactctcat ctttttttcg gaggtataaa aaccgcttga tcttctgggt
     5101 gggggtgcgt tcaaacgcat cctcctgcag gtggaccttg ctgaggcggc tgaagacgtt
     5161 cagctccttg ttcacctctt cccgtatctt gttgagatag cgggcagctt cgctcttcgc
     5221 ttcgttcacg ctcaaagcca tcttctcggc aaacgcctcg atatccagct tgatgagggc
     5281 gacaagcgct ccgccttcgg ggatcaccag cgattcggta acgaagtcct ggttgttgat
     5341 caccgcttcg atcagctcgg gatagatgtt ctcgccgccg ctgcctaaaa tcatcgtctt
     5401 ggtccgcccc ttgatcgcga gccggccttt cttgtcgatg aacccgaggt ctccggtctt
     5461 gaagaacccg tcgggggtga acgcctcttc attcaaggcg gggttgttcc aatatccctg
     5521 catcaccgat ggtcctttga cggcgatctc gccgatcccg gtagcggggt ccttttcctc
     5581 cagccggact tcaaggtgct ccacgatctt tccgatcaca ccgacggtat ggttcttcgg
     5641 ctttcccccg gagatcaggg gggaggtttc cgtcaagccg tagccgaggg cgtagggaaa
     5701 cttcgcttcc gccagaaacg cctcgacctc ccggtcaagc ggagcgcccc cgatcccgaa
     5761 gaagcggact cggccgccca ttgtaacgat gagcttccgc ccgatgatcc ggaagatgaa
     5821 ccggcgggtg attccgaagc gcaggtagcg ttgaagcttc gggttcgcct tcaacgtcgg
     5881 caggacgctg gagcggtaga tcttctcgat cagcagcggg acggtcatga tgatatgggg
     5941 ccggacttcc ttgagcgcgg ggagcaggct ggagggagcg ggaggacggc cgaggtagac
     6001 gacctcgcag ccgcagagca gcgccaagac ctggccgatg gtgaattcgt acacatggct
     6061 gaccggtaga atggaaagcg ccttgtaccc cggcttgacc ttgaaataca catcggatga
     6121 gatgtcggca ttccacacca ggttcctgtg ggtcagcatc accgccttgc tcatccccgt
     6181 cgttccgctg gtatagatca gcgaagccgg atcctcctcc ttggggcgat gctcggaaag
     6241 gagttgccgg gctttggcat ccaccttccg gcgagtgata tcccgcccgg gcgcattgag
     6301 aaattgattc ctgtctttca gttccccgct gatcggcatc gggatgtgga acaggtcctc
     6361 caagcggatc agaaactccg gtttcttccg gatgaaggga agaatcttct cgacatggcg
     6421 ggcgttgacc acgaccgcct tcaccccgcc gtcctgaagg atatgaagga tctctttggg
     6481 tgaaaactcg ggaagaacag gaacggcggt aaggccgatg cccgtcaggc cgaagtatgc
     6541 aagcatccaa ttggggcatg attcaccgat gagggcgatt cggtcccctt tgacgaatcc
     6601 ctgctcaaga aaatacaagc tgaccgcatc gcttttgcgc ttcaattcac tgtaggtgag
     6661 ctcgcttccc tcttcggcgt aggttcggag ggcgatgcgc ttgggatagc ggctggcgct
     6721 gccggcgacg acccgctcca tcgtatactt gtccacgagc ttcatcttca gcccgtcgga
     6781 tcgcttcatt cgtttgatct ttttcttact gataaccggt tctttcttct tctttttcac
     6841 tgtgcagctc caatcgttcc gtaccagcgg ttcttttcac acacgacaaa gggagttgag
     6901 acacgacctt tggcctgccc ttccaagagt ctatgatcat tgtttgtact cgagagatgg
     6961 acaaagtaga gctcattgcc ggcaaacccg ctttcctgaa caaatgtgag ggcatcgtcg
     7021 ttggaaagat gtccctccac cgaacggatc cgatgtttca gaatacgggg atacggtccc
     7081 tcttccagca tcttttcatc gtaattcgct tccaaaaaga ggatgcttgc cctttgggcg
     7141 agttctttct gctcttcggt cgttttgcca aggtccgtca agagcatcac ccgctcttcg
     7201 ggatggtcga tataccatcc gaccgaaccg gggctgtcgt ggcttgtcgt aaaacaaaag
     7261 atcctcatcg cccccagtcg gatcacctcc ccgacttcga cttccaccac ttgtttttgg
     7321 ggaatgccga gaaaggcgaa ctcacgggcg ttgcgggcga tcgctttcgt atggaggtgg
     7381 atcgggatat cccggcttcg ggcaagaatt cccacccctt ttgcatgatc ggggtggaga
     7441 tgggtgacaa agaccgcatc aagctcgctt gccgaagagc cgaatcgatc aaggcgtcga
     7501 ttcagctcga caagggagaa cccctgatcg agaagaatcg aggtttctcc atcgctcatg
     7561 tagtaggcgt taccactcga gccgctgccg agaaccgccc gcttaagcat ccgaagcccc
     7621 ccatgcgcga agaatcgctt catcatagcc gaggtgggtc ttcccgcgaa agcggatgat
     7681 gggggtctta tgcagcgccg gcttttccct cagttcttca agcggatcgt agacccgata
     7741 cgcataccct tctttcctgt acaccttcga agcggtgtcg atgagttctt cagctcggta
     7801 ggtggtaaga atcgaggcaa tctctccggg agaaagcgaa cgctcattta aatcgacgaa
     7861 gtggtgggca atccgggctt ccctgcagaa gcgaattgct ttttttgtct cattgcactg
     7921 tttggtcccg ataatttgga tcataccccc ctgcctcgct gaaagtgtat cggaggcagg
     7981 gtttaattgc aattcaaccg gcgaagagtt cgttgatctc ctcttcgtac atcctatcga
     8041 tctcgaaccg cttcagctcc tgcttcgctg aaagctcctt tcccacttca aagcttttgg
     8101 aaagcagctt gaagcgggcg atgtgttcaa agggcttgaa cccgcttttt gtgctgatga
     8161 cgtcattgat cgtccgaccg atcagctctt ttacttcgcc gaggtgggtc atcgtctctt
     8221 caagatacgg gatgtttttc tctttcaggt attcggcgac ccgggccttg ttcaacacga
     8281 tcagcgcacc gagatacttt cgatcctgcc ccagcaccac cgcctgctcg atgtactcgc
     8341 tttccgcaag cttcgtctcg atgggaacag gctccaggtt ttctcctccg gcgagaacga
     8401 ttgtgtcttt cgcccggccg acaattgcaa actcgccgct gtgggtccac atcgccaggt
     8461 ctccggtatt gagccatccg tcctccgaga gaaccgtctt ggtgagttca ggccgtttga
     8521 aatacccgct catcacctga gggccgcgga cgtacaacac gcccttctcg cccgggggca
     8581 cggcttggcc ttcatcatca aggatcctga gctccgttcc ctccaagccg gtcattgtcc
     8641 gcttgatgct tttttgaaga gggcggattg aaaccaccgg agcgctctcg gtcagcccgt
     8701 atccgtcgac gagcttgacg ccgatcgaat caaagaagaa atcgacgctt tcactcaagg
     8761 agccgccgcc gctgacgccg gcgatgaagc ttgttccgag cttcgcccgg atttttgaaa
     8821 acaccagaac gtttccaagc ttgtagaggg ggaagagcaa cactgcagga agtattgagc
     8881 gaacgatgtc gacgaagcgg ttccgtttcc tgaacgtcgc caccctgcca aggagcagat
     8941 cgcggttctt cgcatatgtt gcggcgacac cggtaaagaa gaaaaagaga gccgctttga
     9001 tcccgccggt cttcttcatc gaggcgaaca ccccgctgcg gaccgcttcc cagatgcggg
     9061 gaaccgagcc catccaatgg ggattcacaa cagccaagtc attgagaaga attgaaccga
     9121 ggggcttgga gtaagcgatc gtcgaagcgt tggaaacggc gacatattga agaatccgct
     9181 caaaggagtg ccagaccggc aggacgctga gccaccgctg ctccggctcc aaccccttga
     9241 tcagcttggg aatcgcctcg agctggtaga cgaagtttcg atggctgagc atgacgcctt
     9301 tgggttcgcc ggtggtcccg ctggtgaaga tgatcgtggc aatctcctca gcatcgcctg
     9361 ttgcgatcgc ttcctgaatc gctttacgct ttttggaatc ttgaagcaga aggttgcctc
     9421 cggcgatcag ctcgctgtac agaagaacgg taatgtcgct tttgatcgag aggtcccgga
     9481 cctgctcctt cgtcgtcttc cgatcgagca cagcgatggt ggcaagatga ggaagctgat
     9541 ccagcagcga agcgaccttc ttcagctggg cgatgttttc aacgaaacag aactccgctt
     9601 ccgtgatgcc gaggatgaac gccacttcgt gggtcatcgc atcctggccg cggggaacgt
     9661 cggcggctcc gagggaaaga atcgccagat cgcttgcaag ccactccttg cggttgtcgc
     9721 tgatgagtcc gacccggtcg ccgcgcttga ttccataaaa tgagagagcg gctgccaagg
     9781 cgtacacttc atcatggagt tgacgatagg tgatcggttg gaatgttccc ttctcgtcct
     9841 tgctgagctg cacggcgacg gatggatatt tctctacgat ggtttcaaat agttggggaa
     9901 ttgtctgata catgaagacc ctccaagggc tgatactgat atttatatat acatttttac
     9961 aaattctgtc aatctgtata ttaatgcttc tcattgactt gtttcaccca aacgcttcat
    10021 aatagcgcaa agtcgagagg aagcgatatg aaagaacgaa ttgttgatgt gctgaggaaa
    10081 gatccttcag atgagctgat tgcgattgaa ggatgggttc gaacgaagcg ggactccaaa
    10141 aacgtgtgtt ttctggaggt caatgacggc tcttcattaa aggggatcca gatcgtgttt
    10201 gacaagaaca ccttttccga tctcgctgct ctcaataatg ttacgaccgg gagtgcggtc
    10261 atctgcgaag gaacgctcgt tgcaagcggt ggcggaggcc agagcgtcga gctggccgca
    10321 agcgccctca cgctggttgg cgaatgcccg acggatacct atccccttca gaagaaacgg
    10381 cacaccttgg aatacctcag ggaaatcgcc cacctcaggg ctcgaaccaa cacgttcgga
    10441 gcggttgccc gggtgcggaa cacccttgcc tgggcgacgc acaaattctt tcaggataaa
    10501 aacttcttat atgtcaacac cccgatcatc tcctccagcg acgccgaagg agccggagag
    10561 atgttccgtg tcaccacgct ggaccttctg aacgtgccga agaccgaaga cgggcaggtc
    10621 gattacacca aggacttctt cggaaagcag accttcctga cggtcagcgg tcagcttgaa
    10681 ggggaaacct acgccttggc gctgaagaac atctatacct tcggccccac cttccgggcg
    10741 gagaactcca acaccaagcg ccacctctcc gagttctgga tgatcgagcc ggagatggcc
    10801 ttctgcgata ttcatggaaa catggcgctt gccgaagagt atgtgaaata cctcttcagc
    10861 gaagtgctca ggaagaacga agaagatatg gagttcttca gccgccatta tgaagcggag
    10921 atgggtacca cgcttcagaa gatcatcgaa accccgttca tccggctgac ctacaccgat
    10981 gcggtcaagg agctggcaaa gaaaaacgac accttcgagt ttcccgtctc ctggggaacg
    11041 gatctgcaaa gcgagcatga gaaatacctt accgaggtcg tcttcaagtc tccggtgatc
    11101 gtcaccgact acccgaagga gatcaaagcg ttctacatga agctgaacga tgatgagaag
    11161 acggtccggg ggatggatgt cctcgttccc cgcctgggcg agatcatcgg cggcagcgag
    11221 cgtgaagcga accttgagat cctggaaagc cggatgaggg ctctgaagct taatcccgag
    11281 gactattggt ggtatctgga tcttcggcgc ttcggttcag tcccccactc cggcttcggt
    11341 ctgggcttcg agcgggcggt ccaatacgtc accggaatgg ggaacatccg cgatgtcatc
    11401 ccctacccgc gctcacccaa atccgccgag ttctaaatcc tccttacatc cggcgaacca
    11461 tcgcccagat caacagccgg ctcaaggagt tcgtcccgct cttgctgtag aggcttttct
    11521 tgtacgtggt gacggtagcg ttggcgattc cgagggtctt ggcgatctcg gcggtcgcct
    11581 ggccgctgag tatcagggaa aggacctgac gttcccttct ggtgagatga accggctctt
    11641 gtacctccat cactccgtca acagcttgca gaagattggc gatcgcagct cgaatcttgg
    11701 cgggattggc gttcaacgac accgaagcgc accgccaatc gtggaggtag gtgaagactt
    11761 gagccaagtt cggtggcgca aagatgagaa gccgatgttc tttggtgata tccttcattc
    11821 taagggcgtt gggcaggtcc cggcacaaac agatcaaaag gtcgtcggtg ctggcaaagc
    11881 gatcgtcgtc gatcgggcag agggaacatt caagttcggg aagcctgttg gtccgactga
    11941 gcaaaagctc gcgttccaga gtgttgtggt agagtaatcc gatggagtgt ttcattaccc
    12001 cttaaggggc aagcttaccc cttttgcttc aacgccttgg caagctcccg gtcaaacgca
    12061 gcggaaaact ccctgatttc ttttggaccg atcggcttgt gcttctctgc aaacactcca
    12121 tattcacgaa gcgtcgtcat cgcatttctg agcgataatc gctcggcgat gttttcattc
    12181 gtaaagacgt tcccccggtc atccagaacg gtgatcccct ttttgacgag catatccgcc
    12241 aagatgatgt cacgggtgat cgccagcgaa tcgggtgggg catgatcgac gatccatcga
    12301 tccgcctgat cggctccgct ttccacgacg atcatggtga tcgggctttt gatcatccgg
    12361 cgctcttccg gttgctcaac cgcgcttcgc agcagcgcgg tatgctcttc gtatgcctgc
    12421 cggacatctt tcagaaggcg gtcggcgacg aagaacgctt tgagattccg attggtgacc
    12481 gccttcagga tgatcgctct cagcggcttg ggacaggaat cggcgtcgat gtacagctct
    12541 atcatatctt tagaataccg cactttcgtt gcattggaaa ccatgcggaa attaatcatc
    12601 catgacatct tcatggcttg aatcattggc gaccgctcaa tgcgaaatat cgccggaggg
    12661 tacttcgaag aacaccgctc ttgaactgga gcggtgtttt attctaatgt tcatgaagtg
    12721 aaagatttct tttcaaggag taccatctat gaacatttca gttttgcagg aagcccgcta
    12781 cggctataag cccaaattgc cgacgatcct tacaagcggc gcatgcaagc tggttcccgt
    12841 ttttggcaag aagacaagca gcgctgccga cagcgaggcg atcgcctccc tctttcccaa
    12901 tacctacggc gcgccgatcg tcagctacgc gcaaggcgag aacaacggag caaaaaccgt
    12961 tcgcaacgtc ggagtgatcc tctccggcgg acaagctccc ggcggacaca acgtgatcag
    13021 cggactcttt gatgcgatgc aggcggcgaa ccccgaaaac aagctctacg gcttcaaggg
    13081 cggtccgtcg ggaatcctca aggatgatta tcggatcatc gacgctgaat acctcaagga
    13141 attcagaaac accggcggtt tcgatatcat cggcagcggc cggaccaagc tggaaacggt
    13201 cgagcagttc gagacctgcg ccaaggtgtg caagaagcat gagatcggcg cgctcatcat
    13261 catcggcgga gatgactcga acaccaacgc cgcggtattg gccgagtact ttctggagaa
    13321 aaaccacggc atccaagtgc ttggctgtcc caagacgatc gacggtgact tgaagaacga
    13381 atcgatcgag atctcgttcg ggttcgatac cgcgacgaag acctacgccg agctggtcgg
    13441 caacatcgcc cgcgatgcga actccgcgaa gaaatactgg cacttcatcc gcctgatggg
    13501 ccgctccgca agccacatct ccctggaggt cgcccttcag acacagccga acttctgcct
    13561 gatcggtgaa gagatcggag cgaagaatca aagcctcgat gatgtcgtcg acgagcttgt
    13621 ccggatcatc gccgcccggg cggatgacgg caaccacttc ggagtcggcc tgattcccga
    13681 aggggtcgtc gagttcatcc ccgagatcaa ggttctgatc caggagatca acaccctcct
    13741 cgccaaggag gagaaagcct ttggtgcgat cgaggatgac catgagaaga tccgattcgt
    13801 aacggatctc ctctccaagg cttccgccca cgtcttctct tccttccccg aagccttcag
    13861 gctccagttg ctggccgacc gcgattccca cggcaacgtc caggtctcga agatcgagac
    13921 ggagaagctt ctctcttcaa tggtcgaaga gcggttgaac aagcttggca agggagcgaa
    13981 gttccagacg cttcaccact tcttcggtta tgagggccgc tgcgccttcc cgaccaactt
    14041 cgatgcggac tactgctaca gcctcggata caacgcgttc gtcctcgtcg cccagaacct
    14101 caccggatac ctcagcagcg tcacacacct cgccaagccc gcttccgagt ggcaggccgg
    14161 cggcatcccc atcacgatga tgatgaacat ggaggttcgc cacggtgaaa gcaagccggt
    14221 catccgcaag gcgctggtcg agctggatgg agaacccttc aagtacttcg catccaagcg
    14281 cgacgagtgg gcccgcaaga ccgcctacac ctaccccgga gcggttcagt acttcgggcc
    14341 tgccgaagtg gccgaccgca ccacgatcac cttggccttg gaacagggca gataggtatt
    14401 ttcgttactg cacgatgcaa ccgcctcgag gggcggttgt tttgtcttcc gatatgatcc
    14461 accatggtga ttattcgttt catgattgac acgcaaatga atcgaatggc tattcattgc
    14521 aaatacgtgt catttgtagg ttaaattctt gaattattcg tctcatttct gaaacgcaaa
    14581 tgaatcgaat aaatattcgt tgcattttta ttgtgtttat gacatcatta cgtaggagaa
    14641 tgatgcatga aagaaagcaa aacattagag ctgaaagaaa acattacaaa tacttttttg
    14701 aaaaccgtca gtgctttcgc caatttcggc gatggtacga ttctcttcgg agtcaatgat
    14761 gaaggaaatg tagtgggaat cgaacatgcc gaggaatcct gcttggatat agaacataaa
    14821 ataaacgaca gcataaaacc gaaaccggat tttacactga gcatcacaaa cgagaatgcc
    14881 atcgaactaa aggtttttga aggaaagtat aaaccgtatg tttacaaggg gaaggcatac
    14941 agaagaagcg gaacatccag cgtcgaggtg gatatgatcg aactaaggcg tttagccttg
    15001 gaaggttcaa acctgtttta tgaccagctc gcctgtgcgg aaagcaacct cagtttttcg
    15061 tatctcgaaa aaaaacttca ccatgttctc gacatcagca ccttggatat cgacatactg
    15121 cgctcgttgg gtttctatac gaaggatatg aagtacaaca acgcagccgc tctgtttgcg
    15181 gatcgaaata cgtttcccgg tgtcgatatc gtccggttcg gtgaaacgat caacgtgatc
    15241 ctcgaaagag agacgatcgg caacacctca cttctcgagc agtacgacac ggcattggat
    15301 cgatacagaa gaaactatca atacgaagtg attgaagggt cgctgcggca aaaacgtgag
    15361 ctcgtaccgg aggatgcatt cagggaagcc cttgcaaacg ccttggttca tcgagactat
    15421 gacgtgcatg cccatatccg gatagcgatg ttcaaacata aaatcgaaat ttcttcgccg
    15481 ggcggactcc ccttcggagt caaaaaagaa gaatacctgc atggaactct ttcagtattg
    15541 aaaaatccca ttataggcaa tttgtttttc agactggggt taatagaaac tcttggaaca
    15601 ggaatcaggc gtataaacga agcatacaga gagcatgaca taaagccgac gtacaccata
    15661 ttggatcaga gcataacagt cgaactcccc tccacgacag cggaatatga agtaaccggt
    15721 gatgagtctg aaatcattga agccttacaa ggcggagcaa tcctgtccag cagtgaaata
    15781 gcggacagga cagggttcag caaaagcaaa gtggtgaggc tcatgaagca tctggaagag
    15841 aagcggtatg tctccgtttc cggagggggc agaagcacga agtattcgct gagatgatga
    15901 tcagggcctg attcagcacg atctccgtcc gcctggtcgg atcggtgcac gattccataa
    15961 cctgacgttg gagcagtatg tcctataaaa ataatataat aacggcttga catataataa
    16021 gtgtttatat acagtatata cattcattac acaaagaaca catcagcgta tatgggggga
    16081 tacagtgaat atacagataa gcaatgcaag caacgaccct atttatgtgc agataaagaa
    16141 tcagatcaaa tcagccatca tctccggaag catggctgcc ggagaacaat taccgtcaat
    16201 cagatatctg gcgaaggaat tgcgagtcag cgtaataact accaaacgag cctatgacga
    16261 actggaagcg gaaggcttca taaactccgt tcagggcaga gggagctatg tctctcttca
    16321 aaacgaggag tttatccgag aagagcaatt acgaaaggtg gaggcgtcat tattggaagc
    16381 gatcaaagga gccaaacttg ccggattgtc aatgaaagac ttgaaagaaa tattggatac
    16441 gttggaagga gttgaagatg atgcataacg caatcgaaat acgaaacctg acgaaaaact
    16501 ataaaggttt ttcattggga cctttggatt tcacgattcc caagggctgc atggtcgggt
    16561 atatcggaga aaacggcgcc ggcaaaagta ccacactgaa agccatttta ggcctgatac
    16621 atgctgacag tggggaaata aagatttttg gtaacagtac gaacgaacat gagcccgagc
    16681 tcatgaataa aatcggagtg gtttttgatg ccatcaacct tcctgccgat atgaaggtcc
    16741 aagaagtggg aacattctgt aaactcgcct ttaccacatg ggcagaaaac acattcaacg
    16801 actatctgaa gagatttaca ctgccggatg ataaaaaggt gaaagcgctg tcccgtggaa
    16861 tgaaaatgaa actctcctta tccatcgccc tttcccatca ggcggagctg ttgatccttg
    16921 acgaggcgac aagcgggctt gatcctgtag tgagagatga aatcctggac atccttttgg
    16981 attttctgca ggacgaaaac catacggtat tgatatcctc acacatcctg tccgatctgg
    17041 aaaaagcggc tgactatatc gcgtttcttc acaatggaaa actcctgttc atggaggaaa
    17101 aagatcttct tgtcgaacgg tacgcattat gttccttgga tgatgaaacg atcggacatc
    17161 tcgatcctga agcgatcgtc ggtaggcgca ggcatgcctt tggccaagat ctgctcgtca
    17221 ttcggaacct gatgccgaag agcatcaaac tgacgaacgc aagcatagaa gacatcatgg
    17281 tgtatatcat caagggggtg gaaagtgaaa gcgttgctct ataaagattt ggtttcctcg
    17341 aaatcaacat atctcttgct gttgatcatg gtaggcgtga ttgccggtat cgcaatcaca
    17401 cagggagcga tgatcatcat tcctttcatc ttcgcctata tacccatgat actgggggca
    17461 atctccttcg gatatgaaag ccagtcggaa tttcccaagt tcctgttcac aacgccgata
    17521 tcacgtacaa cgtatgtgaa aagcaaatac tgtttctccg ttctcttcgg aataatggca
    17581 ttcgtgtcgg ggttggcggt tttcggcatg gaatacggga gtttggattt tgctctcatc
    17641 atggcagcca catcgtttgc cgtaccgatc atattcgtgg cgctacagat tccgtttgtt
    17701 tttgaatatg gcgtggaaaa agagggaatc atcatggtgg cgacgtatgc cgtcctgttc
    17761 attacgatcc gtcttgtcgg gaagcatctg aatcggatga tgaatctggt tcaaacgatg
    17821 aaccggatca attcgtatct gatttccgta gcgatcgtgg caggagcgat ctgcatcttg
    17881 gtggtctcaa tccatatgag ctgtgtcatt gtaaaaagga aggaatactg aaacggatat
    17941 ggcgatatcg acctgatcac cgccactgga tcagggcctg attcagcgag atgtccaccc
    18001 gcttggtcgg atcggtgtac gatcccattc cgtggcccgg gagcagcacg tcaaagggta
    18061 ttccttggag cttgaagagc gatgcgatgt agcgctgctt gtcaaagtcc accgacccgt
    18121 cccacccgaa gtccccccac ccgagggtgc cgatcacatc gccggagaag agaatcctcc
    18181 ggtcttcgaa cgtgaggagg tacgccatgc agcccaaggt gtgaccggga agatggatcg
    18241 cctcgaattc cagggcatcg atcgacacca tctccccgtc gcctacgatc cgatcgacgg
    18301 ttaccggagt catctccctg tggtacaaga agcccgcgca ccgatgatcg gcggaggcca
    18361 ccgagtcggc ggtctccttg cttgacagga tttccactcc ccgatctttg aagaggcggg
    18421 ctccccctgc gtggtcccaa tgggcgtggg tgagaaacag ggttgagatc ttatcgggat
    18481 cgaggcccca catctccatc cgcttgaaga tgaggccgat gttctccccg ttccccgcat
    18541 cgatgaggat gtatgaagac ccgccgtcga cgaggtagct gttgcactcg tcccacggac
    18601 cgaggatctg atcaccgccg aggccggcca atgatccgga aagctgatag atatccggca
    18661 gcagctgcat gtgcgacccc taggaaaaga gcgcctcttc gagccggatg cacaggtagt
    18721 ggtagatcgg caggtgaagc tcttgaacct tgtacgtttc gctttctccc accctgatcg
    18781 caacatccgc ctttttggcc agctcgccgc cgtcgctgcc ggtaaaggca agaaccttca
    18841 tccctttcgc cttggcgacg cttgcggcaa ggttcacgtt ttttgcgttg ccgctggtcg
    18901 agatgcccca gaagactccc ccttcctcgc cccagccgag ggtaagctgg gcgaaggtga
    18961 ggttgggatc gacatcattg gcaaacgcgc tgctcaagga agcgtgggtc ggaagagcga
    19021 tcgccttgat cggactctga agcttcatcg ccagatacga gccatgctcc tggtccacct
    19081 cgatgagtgt ttccgccacg attcgctcga tcggccgctg tcggatgaac gatttcatca
    19141 gctccccgac gatatgctcg ctgtccgccg ccgatccgcc gtttccggct acaagaatca
    19201 tcttgccttt ctttgcggct ttgaccaaaa ggtccgccgc agcttcaatc tctttcctga
    19261 ttccctcaag ctgaggatat cgttcataca cgtcaagcat tgtgtgtgct cctttggtac
    19321 ccgtcccggg cgactccgag cgccgccatg tctccaagcc gatcaccgag accggaggga
    19381 agaatcgtgc aggattcccg gcttgtctga agagcttctt tttcgatgac ctcttccatg
    19441 atcgaccgga acaggtgttc gctgcgctcg aaaatgcttc cgatgatgat ccgttcgaca
    19501 ttcagcagat cgatcaggag cgcgatcccc aatccgagct tggcggcgct ttcttcgagc
    19561 accttctttg ccgccgggtc gttcgcatcg gcaagggcgc atacctcttt tgccgaacgt
    19621 tcctgtcctg tggcgatctt gtataaaccg ctgatgccgc atccgctgca atagctctcc
    19681 cacgagcctt ttttataata gcacatcggt ccgtcatcgg cgatgcggat gtgccccgcc
    19741 tcgccggcga ggccattgac cccccgatag agccgggaat ccaggatcag ccccgctccg
    19801 aggccggtgc cgaaggtcag gaaaatcatc gacgagctgc cgacccccgc cccccagtac
    19861 cattcggcaa gggcgcaggc gttcgcgtca ttctccagga acgtagggat tccgaaccgc
    19921 tcttcgaaat acttcacgat ttcgatatca tcccacagcg gcaggttcgg cgggctctgg
    19981 atgatcccct tctcgctgtc caaaggacct ccgcagctga ttccgatcgc ccggatcgca
    20041 tcgtcctcct tcttgtatgc ttcgatgatg agggcgcagc gctcaaggac ttcctcgggc
    20101 tttccacggg tcgggaagct ctgtttccgc cggatcgaac cgtcaccgct gccaaggctg
    20161 accgcggtct tcgttccacc gatatccaag ccgacaaaca tcatcgccta ccccttcacc
    20221 gaaccggaga gcagtccctg aatgaagtat ttgccaagga agatatatac gaacagggtg
    20281 ggaacggcgg cgatcaacgc accggccatc tgggcgttcc actcgatgac ctgcgtgccg
    20341 gcgaggtttt gcagcgcgac ggtaaccggc tggatcgacg gtttctgcgt gagcgtcacg
    20401 gcgaagagaa agtcattcca cacgctggtg aactgccaga tcagcacgac gaccatcgtc
    20461 ggtagcgaga tcgggacgac gacttggcgg aagaccccga ccaactgaag accgtcgatc
    20521 attcccgcct ccaccagctc atcggggatc ttcgcgtagt agttgcggaa catcagcgag
    20581 gagatcggaa ggccgtagat cgtatgggta acgatgagcc cctccagctt tccatagagc
    20641 cccaccctat tcagcgtccg caccaaagga atcaggattg attggtaggg aatgaacatc
    20701 ccgaagatga cgagaaaaaa gaggactttt gaatatttga accgcacctt cgcaaagatg
    20761 aatccgatga ttgagccaaa gagcacggag agcaggctca ccgggatgac gaggatgaag
    20821 gagttgatca agttctgccg cagcgccttg aacgccttcg agaacccttc gctcgatgga
    20881 gatctggcca acacccacat ctcgctcacg cggacatcgg caaacggctt gaggctgttg
    20941 ttgatgatca catagagggg gaaaagaaac agaaaggctg caatgacggc aaggatgtat
    21001 ttgacggctc ggttggtctt gctcagtttc atcctcaatc ctccctcagg cttgaagcaa
    21061 ggtagggcac gatgacgatc gcaaccagaa gcagcatgac gatcgagatg gcggcgcctt
    21121 cggcgtagtg gtttcccctg aacgtcgttt cgaacatgaa caagccggga aagtcggtaa
    21181 caaagcgcgg acccttgccg gtcatcgcat aggcaaggtc gaagatcttc agcgagatgt
    21241 ggcccaacac gatcatcgcc gagaaggtga tcggtttgat gatcggaaga atgatccttg
    21301 tgatgatcgc ccactccccc gccccgtcga tctgggcgga ttcgatcagc tggtcatcga
    21361 ttccccgaag accggcgagg aacatcgcca tcgtatatcc gaggtactgc catgatgctc
    21421 cgatgatcag ggcgatcagg gcgacgttga agccggcgat cgaataggtc gaggtgaacc
    21481 acccgaactc cgccttgaac cccatcgttc gaagcagggc gttcaggccg acctcggggt
    21541 tgaagatcca ccgccacagc actcccgtta cgacaaagga gagcgaaagc ggaaagaggt
    21601 acaccgtgcg gaagaaccct tcccccttga ggttcacaaa cagcaggtag gaaagaatga
    21661 atcctcccac gacgcagatg gcgatgaaga agatcgtgaa gtagatcgta ttgacgatgt
    21721 cggtcttgaa gcgcagggag cccatcagct cctggtaatt cctcaggccc acccacgtca
    21781 tgtccggcaa gatcgaatcc caggcggaga atgacacgcg gactgaccag aaaatgaatc
    21841 catagacaaa caggaataat accgcgagga acggtatgag catgataatc gctaagcgct
    21901 tgtctttttg tgaacagtac atgggctccc ttatcataga atggttgcag gttcactgca
    21961 accattctta tcacaggttc tatgtccgaa cgttacggag cataccgatc ggcggccgcc
    22021 tggaattcac gggtcgcctt ggccacatcc agatcggtgg tgaaggcggc catgatgtcg
    22081 tttacccggg tcagccacgg ctcactgatc gcagctccgt gggcgacgct ggccacgatc
    22141 gcattggagg cgaaatcatc catcgcggcg ttgagatagc ggtcatactt cgagcggtcg
    22201 ccgtcggtcc gggcgggaat cgaacccttg atcgggttga atgcgtcctg tccttccttc
    22261 gaggcgcaga gctcgagcca cttgaccacg ttctcccggt tcggcgcgtt cttcggcaag
    22321 ccgaaggagt cgctgagcat gatgaacaca cctccggttc ccggagccgg cgcccaatcc
    22381 caatcctcgc cctgtttgaa gttctgtgag tagtaatacc cggcagccca gtcacccatg
    22441 acgttcatcg ccgctttgcc ttcggcgaca tagccggcgg catccatgtt cgccagagcg
    22501 gcgtggtcgc ggttggtgta cttcatcatc ttggtgtagt tgaccaatgc ttcctggact
    22561 ttgggatcgt tccacttgac ctttccgttc cacagatcgc ggtagcggtc cgggccgagc
    22621 gaagccagaa tgacactctc caaaaggtgg gtggcagccc agctctcatt gtctccgagg
    22681 gcgagagcgg gaatgccgtt cttctcgaac acgtcggcga cccggaagaa ttcatccatc
    22741 gtcttcggcg gtttgagatt gaacttcgca aagagcttgg ggctgtacca catgacgttt
    22801 gatcggtgga tgttcacggg aatcgaatac acttcgcctt tataggagat gatgtcgatc
    22861 acgtcggccg ggaacttgtc catgaatccg gcatccttga ggatgaaggt gaccggttcc
    22921 atgaagcccg ttgtcaccca cgtgtcgatc aattcgtgtc cggcgtggac ctggaacgta
    22981 tcggggggat ttccaccctg catccgcgtt gcaagcaccg ctttcgcgtt ggttcccgct
    23041 ccgccggcta cggtcgcgtt gatgatttca agctgcggat gtttcttgct gaactccttg
    23101 aacatcgctt caagtccgtc cgcctctcct cctgcagtcc accaactgaa gatctcaatc
    23161 gtattggcgc ctttctgctc cttcgttccg gctgcgaaaa cgagtgccga agcgataagc
    23221 aggaccacca agatggctag aacgtttttt ttcatgatat actccttttt tcatgaatcc
    23281 gacctcgacg aggtccttta ttccatcaac cgttcaaggc ggttgatact taacactact
    23341 gcccggatcg tgtggtaatt cactttccag ctgtggctca tatgcctcca gatcggcgtt
    23401 ccgtcctgct gcagcagagg cagccactcg cccacgtcat ggttgatcat cacatcgaag
    23461 acgaagcgat ggacgttttc atacgccttg atgtagcgct catcaccgta gagaaggtag
    23521 gcatcgagca tcccgatcat gaactcagcc tgctgccaga actccttcgc cttgtcgtgc
    23581 acggcgctcg tggtcagcga accctcgaca tacacgccgc cgtatttctc gtcgactccg
    23641 taggcgacgg agtggtcgag catcttgcgg aacgtcggct cccacgcttt gagcgggacg
    23701 tcagcgctct tgagtgcttc ggcaagcagc cagaagagtt cggcgttgtg gccgtatgag
    23761 gtattgtcaa gggcgtgctc cttctccccg ccttcccctt caaagcggtc ccatccccag
    23821 atgatgtcga acttgatgtg cggggcgatc gtccaatcct cccagaacat gggaatgccg
    23881 gtcttgaact ccggatgaag aatcttctca tcgatctggg tgatgatctc aaggagcttg
    23941 cgcctgtgga tctccttgcc gcttgcctga tacagcgcgg tgaacgcttc catcaggtgc
    24001 atgtggatgt ccagcgtctt gcgatccccg ccggcgctgc cgggcccgca gaggctccag
    24061 ttccgattca gcatcgtcca gtatccgccg tagttcgtat cgacgacgta gacttgcagc
    24121 aggtcgaaaa cccgctcggc gtactcgagg ccgatcggat cgccgaacgc cagcgtatag
    24181 gtagagaggg catagatcac gaagctgtgt ccgtaggtga tcttctggtc gatgagcacg
    24241 ttgttcttcc ggtcgaacag ccagtagaat ccgccgtatt tctcatccca ataatgctca
    24301 atcgcgaagt gcaccccttt ggtggcaagg gactggcagc gtccgtcgtg gtcatggccg
    24361 tgcagcgccg ccagtgaaag cgaatagatc gagcggaaat gggcgagcat cgacttttca
    24421 tcgacgccgc tgtccttgcc gtctttatcc cactgggtga ggaatccgcc gtgttcttca
    24481 tcccaaagac ggtcaagcca gaacggcaac agctccttgt ccaggtgatg ctgcagttca
    24541 tccttccacg ctgtaaactt cttcttcata tcgattcatc ctttcctttt gagagcggta
    24601 tagagattca caaacagtga ccggttgtcg atgcagaggt aatggctttc caggcccatc
    24661 cggctgaatg ttttcatgaa gcgctggtag tacgccgggt tgtcttcagg cggctcgcct
    24721 cggttccaat cccaggttcc tttttgaaga tccaccaccc tgatgaccgc atgatcgatc
    24781 cggacgccgg tgttgcgaac catggaaagc gctttttcaa agatcatcgg actcatcacc
    24841 gccgatccga cggagagata gacaccccct tcgagccggg cgatcgaatc gacgaagaca
    24901 agaaaatccc gctcggcggt ccgtccgatc gcagatcccc tgttcgcctt gtgggtgtag
    24961 atgatgtcgt gaccgaacat cggatggccg gtgaagcgga catcgcttac ataggcgcag
    25021 tgctgaagcg aatattcctt gtatgggtgg tcgatgccca gccaacccgg ctgaagatcc
    25081 agcttgacga tcagatcgag caggtccaag gcaccgcttc gtttctcgaa ggatgcgctt
    25141 tgatcgaggg aggtttccag gagctcttct tgtgtgggga tcatcacccc atcctcttcg
    25201 atcatcttcc cgatcgattc gccgtagccg cagccttcga aagctccgac cgcaagcgct
    25261 agattcaggg aaaagcccgt ctcctcccac gtgccgaact tcccttcggc cacgttcgct
    25321 ttgacatcct cgctggatcg gccctgatag gcgaactccc agtcatggat gatgcccgct
    25381 ccgttggtcg ccagatgggt gaaatacccc tcctcggcaa agcggccgag caattgtccc
    25441 agcccgttct tgatcgcatg ggcaccgaac gccagaatca ccggatgctt gtttctccga
    25501 gcttcaagaa tctcgtcaca cagggcgtcg atccagacct cctgctcatc ggcaagcggg
    25561 aaggaatcat tctccggaag tatggttgca tcttcgatgt ataccttgtt ctttcgttcg
    25621 cgaagaggat aataggtcat tcgagtacga tcaaacatac tgttcactgc cattccaccc
    25681 caaaagattc atcagctcct tcgaggaagc gaaatcgggc atcagcagcg aagcgccgcc
    25741 gagaacgagg cgctggcgct tggcgaggtt ttcacctgat cggcgaactt catcgctcaa
    25801 gagtccaacg gccaagaccc cccggctgcg ggcttcgcgc atctcgaccg ggccgtcacc
    25861 aaagacaatg atatcacctt cctgaccggc aagcgaggtg atcagtcgac gtaaaacgac
    25921 ctttttcggg tcttcgtcga tcttccccac cgaaccgtag attcgccctt caaacagatc
    25981 ggcatagccg agcagcgcag cttccctgat cacgtccgcc tgatcggttc cgctggcgag
    26041 atagagcttc accccccgct catacaggta atggaggaac tcgaccgaac cggcgatcgt
    26101 tacctcgctt ggcgggatcg ttccattcgc cacacccgct tttttcgagg cgatcatcgc
    26161 aagcagatcg tcgttgtaga tattcttgta ctcctgagcg ctcagaatct catcggaggg
    26221 tacatagcca taccgcgcta ccatctctgt gaggtagacc atctgttcga tcgtctggat
    26281 accggtcgtc tgatcgatga aatgagcgat ctccgcttcg agcttcagaa tctcttcgtt
    26341 tgtgagggtg gaaagagcag aaccggcgat tgcttccttc atcatcttcg ccatcacccc
    26401 ttcccacccc gagcgcatcg tcgagatcgt tccgtcatga tcgaagatcg cgattttggg
    26461 caatccccgc ttggacacgg gatcgatgat ctcgatcgtc ccgccggagt cgaaaacccg
    26521 gtttgcgtca tccgcggctt tctccgggtt gtaccgccag gcggctttct ccttgagcgt
    26581 aagaatctcc tcgacggtgg gatgtccgca cccatagagg gtcaacaggg attgcgccgc
    26641 gctgagattt ccaacttcaa tgccttctgc aagagatgct ccccgactca tggaaagggc
    26701 catcgccgca aggaacgcat ccccggcacc gacgatgtcg atgtgctgag taaaatgaac
    26761 ccccagaacc gaagagaaga catctccgtc aaagccgacc gccccgtttt ccccgagcgt
    26821 gacgacggtc ggcttgccga agtatccgta gagcgccttg acgagctcct tggtggaaag
    26881 ggaagcgaaa ccgggagaca acgcccgtcc ctcggcttca ttcaccttgt ggatcgcgtt
    26941 ccggtagacg gagttgagct tccggcagtc ggcaaaccac agcaccttct ccccatattc
    27001 atcaagaatc cgattgagcc gttcctggaa gaccttgctg tgcaaccctt ggggaacctg
    27061 ctcgttgatg atgatcgcgt cgtaggaatc gatcttcttt cgaagggtct caaggagcag
    27121 atcgatcgac cgctcaccgg gaacgttgaa gttcccgctg tcataacggg gatgttcgat
    27181 tccctgttca aagactttgt ggtacacatg ggttgcccac ccctcccctt gggtctgcac
    27241 tccctcaata gtaaggttat ggctttcaag gaggtcacag agcaccttgc cgtagaggtc
    27301 ggttcccacg attccataca tgtccacccg tcgggctccg agggttttga ggttcagccc
    27361 gacattgcag ccgccgccga gctctccttt gacgctttga accgaatacg tctgaagccc
    27421 cgtttcaacg gagatttctc ccccttcggt ggtaacctga tacgccaaat cgaggcataa
    27481 atcaccgatc accgccaagg caaccggttt ggatggagaa agaaatgtct tcaaagcatc
    27541 agctttccgc ataatgaaac cgctttcatt ttcatgatag aacctcccct gtgcgttgtc
    27601 aacaaaaaga ttgacaggca ccttgaaaat acctaaggtc taggtatgaa gcacaagacg
    27661 atcagcgata tcgcccggga agcgggtgta tcgaaggcta ccgtctcccg ggttctcacc
    27721 caccccgagt tggtcaagcc ggcaacacag gagcgggtaa aaagagtgat ggagaagcat
    27781 gaatatgtcc ccaatcacat cgcccagagc cttgccggca atcccaccaa gatgatcggg
    27841 gtggtgatcg acgaactctc aaacttcttt ttcatcgagg tcgccgaagg aatcagcaac
    27901 atcctctccc cccttggtta ctcgatgctc cttgtttctt cccgctggat cgaagaagag
    27961 gaaacccgtc tggtgaaatc cctcatctcc agtcaagttg acggagtgct gcttgctccg
    28021 gtcgccaaag agagtaaagc gcttcacctg ctgaaagcca gtcaaacccc ctgcgtgatt
    28081 atcaaccaga ttcccagtga tgcctccctg tcgttcgtgg cgggagacga ccttgaaggc
    28141 gggaagatcg ccgcggctct ggtgaatgag caggaggccg agcaggtcat cgtcatcacc
    28201 ggcttcgccc accagagtat tgaagatcga gtggcgggct tcaaaaaagt gctcgatccc
    28261 cgctttcggt ataagcgcta tcgctttgtg gaaacacagg cggatggagc cgcgctggtc
    28321 gccgccctta tcgataaaga agcgcttgac aaaaagaaga cgattctctt cgtcaccaac
    28381 gacaatgtgg cgatcggaat tgtcaatgaa ctggttgagc agggaatttc aattcccgag
    28441 caagtgaagg tcatcggcta cgacaacatc cgggccacct cgttttgtcg aattcccctg
    28501 acgaccgtca gtcagtcaac cattcacatc ggtgaacagg ccgccaaggc ccttcttcag
    28561 cttatcaaca aggagcgatc ggaaccgctg catcaactca tcgagcccgt ccttgttcgc
    28621 aggcactcct tgtaaaacca tcaaatctcc atggataagt gagaaaactc ccctctccta
    28681 tggtatactc gccatggagg caatctcaga tgcatacaat cgacatgaat ggtatttgga
    28741 agctttcccc gcgcgaccgt cacgcaatcg ccccccattg tccctcgttc ctcgaaatcc
    28801 cttctatcga ctgcactctt cccggggatg ttcactctgc cctcctgaag aacggcctca
    28861 ttcccgatcc gttcgtcggc acgaacgaat tggagattca gtgggtcggc aggcatgact
    28921 ggatccttga aagaacattc accctgactg aagaagattt cgcttgtgga accccgttca
    28981 tcacgttcac gatggccgat acgatgatca cgctctttat caacgatcgt caagtcggaa
    29041 cgatgtacaa tcaattccga aggtggcgct ttgaaatcgg agaacacctt caagtcggag
    29101 agaatacgat caaactcatc tttgaaagcg ccgagaatgc agcgatcagg gaaagcaggc
    29161 agattccata ttccgagtac ccggttcatg cgaaacatcg gaacctgatc cgcaagactc
    29221 aatgccacag cggccgggat tggggtccgt gcatcatgac attcggaatc tacgaaccgg
    29281 tcaagatcga gttcgtcgac gaggggttca tcgacagcgt gaaattcacc acggtcgaaa
    29341 cgggagcatc ctcatttgac gtaacggtcg aggttgaata tgccccgctt gaagcgatcg
    29401 aagcccttga tttcaccgcg acggtcgccg gttcttccca aacggtcacc cagaccttca
    29461 gcccgggacg atcgaatacc gcttcattga cctttgccgt atcggatgtc cggcgctggc
    29521 gccccagcgg agaaggaaag gcggtgctct atcccttgac ggtcaccgcc ggctcccaaa
    29581 gcgttactcg actggtcggc ttcagaaccc ttgaggttga aacgatcgag gatggtgagg
    29641 gcggcaagtc gatgaccttc atcgtcaacg gggagcggat ctttgccaag ggagcgaact
    29701 ggattccgat cgactccttc ccttcccgaa tgaccgggga gcgctatgag caccttctcc
    29761 aatcgatggt cgatgcgaac atgaacatgg tccggctttg gggcggaggg atgtacgaac
    29821 acaaatggtt ctacgatctc tgtgatgaga agggaatcct catctggcac gacatgatgt
    29881 tcagctgtgc gatctatccc gccaccgatc ccttcctctc caacgtcgaa gaagagatcc
    29941 gctatcaggt caatcgtctt caagctcacc cctcgatcgc cctgtggtgc ggaaacaacg
    30001 aggacgtcgt cggcaaaacg gtcaaagcgg ccgacccgtc ccgaaggtgg tggccgtcat
    30061 ccccttcaag aagtgaaggt gaacacatcg acaaccgtgg agatatgcat atcccaagcg
    30121 tgtggcatga cgggagcccg tttgagcgct actacagcta tgcgccgcgc tttgtcagcg
    30181 agttcggctt tcaatccttt ccatcattgt cgacggtaaa caggtatgcc gatgaagaga
    30241 tgcacaacct caccagcagc gtgatggagc accaccagaa gcatccccgg ggaaactcga
    30301 tcatcatcga gtatttcacc caatactacc gcctcccgac aagcttcgag cagcttctct
    30361 acctttctca ggttcagcag gcccgggcga tgaagatcgc aatcgaatat tggcgaacgc
    30421 tctatggtcg gacaatggga accctctacc ggcagctgaa tgatatctgg ccgggagctt
    30481 catggtcttc gatcgactac tacggaaagt ggaagctgct gcactatgcc gccaagcgct
    30541 tcttcaaccc catccacccg atcgtctata tgaaagagga tggaattgtt gaagtatacg
    30601 tggtcaatga cagtgcgcgg gagatcgccg atgaagcgaa gctgagtgta aagtactcga
    30661 cctatgaagg gaacaagctt ggaaagagcg agtatccggt ggtcatcaag ccgcgctctt
    30721 ccatccatgt cacaacgatc gacctctcca gaaagagcag gatcgatcgc tcgaacacct
    30781 tcatctacgt gaagctgaag agcgacgagc tgtatatgga aaacaccctg cttctggcga
    30841 agccgaaagc ttcgaagctg cggaatcccg agatcaagac aaccgtggcg aacgctcccg
    30901 gcgggttttc cgttaccgtc agctgccgct accccgcctt tgaagtcgcc ctggacagcg
    30961 gctcgatcgc cggcatcttc agcgacaacc tctttgacat ccgccccacc gcccagaagg
    31021 tcattttctt caaaagcgcg aagaaggtga gtctggaagc gtttaaagcg gagctgaagg
    31081 tcttcgacct ctattcgagt agcagatgaa taacggaagg g
//
LOCUS       DAOSLI010000019        30536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1691_length_30536_cov_25.905876, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000019 DAOSLI010000000
VERSION     DAOSLI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 30536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30536
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_30536_cov_25.905876"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLF67_04280"
     CDS             <1..325
                     /locus_tag="PLF67_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89266.1"
                     /translation="RRNGDLLGRSQRPADPARNGSLRSDITSPTSAGNLSSCSSSNLY
                     AGFVGALLSQSMICSSGNSYLDSNICFVCTFTGPGGESPDHVLPEDHRQNDRQSHNHD
                     YGGHH"
     gene            complement(494..1705)
                     /locus_tag="PLF67_04285"
     CDS             complement(494..1705)
                     /locus_tag="PLF67_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE89267.1"
                     /translation="MKLIERPFYLNQLKRVKGVPDIKVITGIRRCGKSKLMASYAEYI
                     RKVDLNANIINIDYSDIEFEELKHYQTLNSYVEEHYCSSKNNYLFIDEVQMCEGFELA
                     LNSLHASEKYDIYVTGSNAFLLSSDLATLFTGRTFSIELFPFSLREYMDYFEQSDVYE
                     AFIEYRKTGGMSGSYVYHDEASRSRYLNDVFDTLVIRDIVQKYKIRNLPLLEKITDFL
                     MNNVSNLVSANKMANTLISNRIKTNDRTVSAYISHLCHAFAFYRIRRYDIKRKKYLAS
                     NDKYYLADPAFRNARLGSRNPDFGRMSENLVALELLRRGYEVYAGVLYKKEIDFVAIR
                     QNEKLYIQVAEFIDNPETFEREYSSLLAINDAYPKMILARTRHEPYQYEGIIIKDIAD
                     WLYGESETNSP"
     gene            2141..2995
                     /locus_tag="PLF67_04290"
     CDS             2141..2995
                     /locus_tag="PLF67_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOE89268.1"
                     /translation="MANGIHDISALFTIKERYDDLSAKEKQIADFILEHPRESVNPSI
                     EELAERIGISESTMVRFARKLGYSGYQRFRIALARETVPVTSQLFEREVIDASDGFDA
                     VFNSAIASLLETQKSLDRGLVLGVATLMANARNVYLMGLGGSNIVAQDALHKLVRTGI
                     NCQWAADFHLQLMMASQGTKEDVALLISHTGSGYDSIALAEELKANQVKLVVLTSNPR
                     SPLARLADYILTVKTGASGAVSESFSARIVALAIIDLLYIHVMELLDERGIEQLNRMR
                     ASIAKRRV"
     gene            3014..4462
                     /gene="gnd"
                     /locus_tag="PLF67_04295"
     CDS             3014..4462
                     /gene="gnd"
                     /locus_tag="PLF67_04295"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOE89269.1"
                     /translation="MKQAQIGLIGLAVMGENLVLNLESKGFSVAVYNRNTERVDNFLA
                     SRAKGKAIIGAHSVEELVSKVQSPRKIMLMIKAGDPVDQMIESLIPLLEAGDTIIDGG
                     NSNWEDTERRVAYVESKGLLYIGTGVSGGEEGALLGPSIMPGGSKKAWPALKPMLQGI
                     SAHVDGIPCCDWVGSGGAGHFVKMVHNGIEYGDMQLICEVYDIQRRLLGLSNKEMHKI
                     FAEWNEGELDSYLIEITKDILAYQEDDGTFLVDKILDAAGQKGTGKWTGISALHEGVP
                     LTLIVESVFARSVSALKEQRVAASKVFDLEIKAPAFDKKQVITQLQEALFAAKIISYA
                     QGFDLIKTAGITNKWDLDLGGIAQLWRGGCIIRSRFLGKITEAYRKNPDLPNLILDAH
                     FAEILKKNHQSLRAIVALAAQSGIPTPSLSAALSWFDSYRTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGRGGSTASTIYSI"
     gene            4473..5558
                     /locus_tag="PLF67_04300"
     CDS             4473..5558
                     /locus_tag="PLF67_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOE89270.1"
                     /translation="MKTLLAKDKAARDLLSLGALVIRLDPGTTPFEYASSFDLHVSGG
                     EFNVAANLSRAFGQRTAIATAMVDYPLGLKIEAEVRRMGVHPFYKRFEHDGVRGPNMA
                     HVYSDRGQGMRAPVVFYNRCDEAAARLDADSFDYDKLFEGGVRWVHSGGIFAALSKTT
                     PELIKEFFRRAKAQGAFVSFDLNYRQKLWSSLSDEPQKAAQKTFSEIVQYVDVLVGNE
                     EDLQMGLGLKGPEVHSTDKLDPTAFWGMAEQAKKKFPNLKAVGTTMRVVHSTNRHDWS
                     AVLYLDGKGYQAPTAQLDILDRVGGGDGFASGLIYGLLDGREPQEALNLGWAHGALLT
                     TYPGDTTMARLHEVEAFASGGSARIQR"
     gene            5562..6596
                     /locus_tag="PLF67_04305"
     CDS             5562..6596
                     /locus_tag="PLF67_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOE89271.1"
                     /translation="MNALVLTEYKNLSYQKVPKPVITSPTQTLVRIKAASICGSDVHG
                     YDGSTGRRQPPLIMGHEGSGVIEEVGSMVTNFKVGDRVTFDSTIFCGACPFCKQGLFN
                     LCDNRRVLGVGCDEYKQDGIFAEYAIIEERVLFHLPEGLSFEEAAMVEPAGVAAHALS
                     LASPSLHDDVAVVGAGLIGLLVIKLLRTVTSGKIIALDIDEKRREMALKVGADYAFDS
                     TDPALSRKIMKLTGDLGLKVVYEAVGATAPIQTAVSLARKGGTVVLVGNVSPKIELPL
                     QSVVTRQIRLQGSCAINGEYPAVLKMMADKVLDVTDLISKTAPLSEGKLWFDRLYNKE
                     GDLLKVVLIP"
     gene            6607..7674
                     /locus_tag="PLF67_04310"
     CDS             6607..7674
                     /locus_tag="PLF67_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE89272.1"
                     /translation="MVRYALLGYGKVAHLHAQALKSVKDAQLVAVWGRNMEKARQFAQ
                     KWKIKAYTDLDQMVKEAKVDAVVVTTVHPLHMEHTLQALDAGLHVMVEKPMALTVRQC
                     DLMIEKAEEKHKKLGVISQRRWYPACQRIRKAIDSGNLGTPVIGQVTMLGWRDKAYYD
                     SDPWRGSWELEGGGVLINQAPHQLDLLHWYLGEIEEVYAQWDNFNHPYIEVEDSAVAT
                     VRFKSGAISSILVSNSQKPGIYAKVHVHGSNAFSAGVQTDGGAMFVAGQSTITDPAVN
                     DLWTIEGEQGNLERWKEEDAKLFREVDATTYFFTLQLEDFTQAILEDRDPASSGYDGR
                     ETVKLIEGIYQSGRTGKPVRY"
     gene            7686..8375
                     /gene="araD"
                     /locus_tag="PLF67_04315"
     CDS             7686..8375
                     /gene="araD"
                     /locus_tag="PLF67_04315"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HOE89273.1"
                     /translation="MHQELQEIVYEANMALKRNGLITLTWGNVSMVDSKREMMAIKPS
                     GVPYESLRPSDIVLLNLKTKEQVKGSLKPSSDTPTHLYLYDHLPEVGAVVHTHSRWAT
                     IFAQSGKAIPAFGTTHADTFYGPVPCTRPLNREETEDHYELNTGKVIVETVDRSNPLA
                     IPAILIEGHGPFAWGKDAKDAVEHAIILEEVAMMAYHTLLLKGNDHPIDQHLLDKHYQ
                     RKHGKNAYYGQ"
     gene            8515..8724
                     /locus_tag="PLF67_04320"
     CDS             8515..8724
                     /locus_tag="PLF67_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE89274.1"
                     /translation="MNELSTLVREYRKQAKLTQEEFALLSGLGIRFVRELEGGKPTVR
                     LDKVNQALALLGFKMAPVPIQLKES"
     gene            8726..9049
                     /locus_tag="PLF67_04325"
     CDS             8726..9049
                     /locus_tag="PLF67_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOE89275.1"
                     /translation="MAYRKVLVLVHNIRAGILEETDDGYTFSYDDEYLARPDAKHVSL
                     TLPLSSTPYHSTTMFPFFDGLIPEGWLLLVALDNWKLDSKDRMGLLNVACRDTIGAVT
                     LENME"
     gene            9046..9981
                     /locus_tag="PLF67_04330"
     CDS             9046..9981
                     /locus_tag="PLF67_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOE89276.1"
                     /translation="MTRCLYCNKPISRDEHDFQWHPACVKKFFGTTSLPTLALDESRL
                     LHSAATSIEQGHAVPGVQKKLALHLLKTRGKGQLTVIGRPLGYIVKLPVKEYPLLPEY
                     EHLTMQLASLARIPTVEHALYRLEDGTLVYLTKRIDRVGETKIAMEDFCQLSGRLTED
                     KYHSSCEQLGKILRRYSRRLLDAVALWHLLLFCFVTGNSDMHLKNFSLYAPNGDEYIL
                     TPAYDLLPTNLILPEDREESALTLRGKKNRLKKEDFVCLAQQYGIHSKTVERLIARIV
                     GLQKTFFEAIEQSFLPKEAQKAYKALIAERIGRLS"
     gene            complement(10000..11007)
                     /gene="yiaK"
                     /locus_tag="PLF67_04335"
     CDS             complement(10000..11007)
                     /gene="yiaK"
                     /locus_tag="PLF67_04335"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF009750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HOE89277.1"
                     /translation="MKISKETMQDVFKTILLSHGFSDSDAAESAAIFVQNSLDGVYSH
                     GVNRFPRVVEYLKKGYIDPNASIEVEGSFGCIERWNANLGMGNLTAKKAMKRAIELAA
                     FHGMGCVAVRNTNHWMRGGTYGWQAAEAGCIGMCFTNTLPNMPAWGARNGRIGNNPFV
                     LGIPRENGKHIVVDMAMAQYSYGKIEEARLADRQLPYPGGYDSDGNLTTDPKAIEETG
                     RVLPIGYWKGSSLALVLDAVTSVLSGGNSTQAIGKLCEDEIAISQTFIAIDPYRLGTR
                     EEAERILEGIIDDLKRSEPIDPDIPVFYPGERTIATRRINTKDGIPVTPGVWEAIMAL
                     R"
     gene            complement(11234..12241)
                     /locus_tag="PLF67_04340"
     CDS             complement(11234..12241)
                     /locus_tag="PLF67_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE89278.1"
                     /translation="MSDYKPPFHMTDNMTSLIAEISEQVGRITVLQEGTVSPHLRREN
                     RIRTIHSSLAIEHNSLSLEQVTAILDGKRVLGNPNEIKEVQNAYEAYELMLRLNPASV
                     DDLLKAHKLMMNGLVPENGKFRSGGVGVFDGEVLIHIAPPAEFVPEHIKNLFSWYQQS
                     ELHPLIKSAVFHYEFEFIHPFADGNGRMGRMWHSLLLGKWKELFFWLPIEKLIQSRQK
                     EYYDALGAADRQADSAGFVELMLEIIRDSLKEVTVVGRSTDQDSDQVTDQDKTPTARL
                     LSVLGDDTLSAAELMDRLGLSHRPTFRKNYLNPALEQELIERTIPDKPNSRNQRYRRR
                     S"
     gene            complement(12402..12569)
                     /locus_tag="PLF67_04345"
     CDS             complement(12402..12569)
                     /locus_tag="PLF67_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89279.1"
                     /translation="MARTKTMASIDAKIEKAQTTVEKTKARYDATIPELEKKNLQAKE
                     LITAIEKSGKR"
     gene            complement(12556..13041)
                     /locus_tag="PLF67_04350"
     CDS             complement(12556..13041)
                     /locus_tag="PLF67_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE89280.1"
                     /translation="MAGFFESCDDEVPLFYEAYPGSIVDISQLQYMLQKAKSYGYRHC
                     GFILDRGYFCKPNIRYMDSCGYEFIIRSRLYTLLKDETERMDKRPNYMTVPAALKELD
                     KIEMVRQYDGVYRLDHAVTKTQKTILKAFGLDSDDVHTTAVSISETLAAQSGGKKYGK
                     N"
     gene            complement(13142..13264)
                     /locus_tag="PLF67_04355"
     CDS             complement(13142..13264)
                     /locus_tag="PLF67_04355"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HOE89281.1"
                     /translation="MNSIFQQSLVLMLKGMVGIFTVIILIYVVIVILNAISKKE"
     gene            complement(13274..14410)
                     /locus_tag="PLF67_04360"
     CDS             complement(13274..14410)
                     /locus_tag="PLF67_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOE89282.1"
                     /translation="MDFLLGGILATTWKDLVMYCVGGILIYLAIRKKLEPSLLLPMGF
                     GAILVNLPFSGAVTQLVEGIGPVQGILDWLFSVGIESAEMMPLLLFVGIGAMIDFGPL
                     LSNPKLILFGAAAQWGIFATISVATLMGFSLADAASIGIIGAADGPTSILVSQVLKSG
                     YVGPIAIAAYSYMALVPIIQPFAIKLVTTKKERMIRMSYNPKSVSKTTKIIFPIVVTI
                     IAGYVAPASVSLVGLLMFGNLIRESTVLDSLSETAQTSLVNLITLLLGITIASTMKAE
                     QFVRLQTLMIMLLGLLAFVFDTIGGVLFAKFLNLFTKKKINPMLGAAGISAFPMSARV
                     IQRMGQEADPQNHLLMHAVGANVAGQIASVVAGGVILGLVPAML"
     gene            complement(14524..16251)
                     /locus_tag="PLF67_04365"
     CDS             complement(14524..16251)
                     /locus_tag="PLF67_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOE89283.1"
                     /translation="MRGGCAYSDFDLEQPPRYQICLQYMADEAEGGGMAGKKQGTFIS
                     DLQKEFAGYNGGKLAKDIVAGLTVAAVALPLALAFAVSCGADAASGIITAIFSGLIIG
                     ALGGASFQISGPTGTMAGVLLIVVANHGHEGVLIVTFLAGIIRILAGVLKVGALVSYI
                     PIPVVTGFTAGVAIIIATGQINSFFGTVSEGVTILERLFSYTRLGFNIDWLSVLFGGF
                     VIALMLLWPKKLNERLPSTLLALIVTMIAQIILDLPVAEVGHIPRSIIGEQRLNLFAL
                     PWKNLGVYISPSISIAALGMVESLLCGTYAEKMKGEKFHATRELYAQGIGNMIIPLFG
                     GIPATAAIARTSVAIRAGQQTRLTSIFHSIALIASVFFLAPFLSRVPLAALAGVLLVT
                     AWRMNDWKSIKQIFSKRMKTSIAQFSITLVATVVFDLTTAILLGIGFSIVMFVIKSNK
                     ITIEIDPVTNRLGKKAKPTKVVYVDGALFFGSQGKLTESIAPLIESGVKRILLSLRGV
                     PNIDHSSITELAEIVRLARKKEVELLFCGVQPRVESMLRRLDFYSLLPEERFYPSVVV
                     ALETIAKEV"
     gene            complement(16248..16916)
                     /locus_tag="PLF67_04370"
     CDS             complement(16248..16916)
                     /locus_tag="PLF67_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE89284.1"
                     /translation="MVVTFLQHASFLVECDKVALLFDYFDGEVTLPENKDLIVFASHR
                     HGDHYNKKIFDLPAKAFVLSDDIPTEDIPHGIVVHRMGSHQRIEVEGLVARTLQSTDE
                     GVAFLLEVEGKVIYFAGDLNNWFWEEEGEAYTSWMEREYHKEIDRLPEAVDLAFVPVD
                     PRLRKRYGLGARDLLERVSVSYLVPMHMWGEFAVGTALKEAVSSYDVEVLAAQRNGQR
                     WRIV"
     gene            complement(16956..18056)
                     /locus_tag="PLF67_04375"
     CDS             complement(16956..18056)
                     /locus_tag="PLF67_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89285.1"
                     /translation="METKVIIINKNALQKFAEGYIECFGHAQAMIIAEEHEHHHYALP
                     ILRSLQKEGVEATGHTFKRAAFNKIFPLLDDKPVVPVFFGDDRLLSVGKRVSYKLNRS
                     FAAIPTVLDSPAIASPWRFIWPGAFIPSQPPTLLLVDTELIRKPKILPQAVAEALLLL
                     ATHGEAVLFGEENLRIDLHTLAWGSADEFFEAYLDNSRYSLELLAKALAQGGVVDQEL
                     EYVTRRNRRVPVSLLALGIFYLGEQLFTLSERDAVGHMKKDREDLIKRLFLPAEQEAL
                     LAKKPKSFQRRPSLNQVKQTLEANILTFGEIKRKLKEAGCAVSVEEARCSLGELKDEL
                     LRMMLRSAHLGVLDYYNELGLLETALTMLEGM"
     gene            complement(18031..18903)
                     /locus_tag="PLF67_04380"
     CDS             complement(18031..18903)
                     /locus_tag="PLF67_04380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE89286.1"
                     /translation="MDNRPTIAIMYDFDKTLTTRAMQEYSFIPNLGMEAAAFWGKANE
                     LARKEGMDSVLAYMKLMLDEAKRHDQPIRRGDFVSLGKTLEFYPGVEAWFDHVNELGR
                     ELGLHIEHFIISSGLSEIIEGSAIGSHFKKIYACEYYYDVNGVAKWPKLAINYTAKTQ
                     FLFRINKGILDVWEDNALNDYTPQELRPVPFVNMIYIGDGLTDVPCMKLVKESGGISI
                     AVHAKGEVDLCHRLMAAGRINFFIEADYTPQGGLFALMKKILARMSSANALEKTHLLM
                     KKEAMSYGNKGHHH"
     gene            19285..19440
                     /locus_tag="PLF67_04385"
                     /pseudo
     CDS             19285..19440
                     /locus_tag="PLF67_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
     gene            19437..19874
                     /gene="fabZ"
                     /locus_tag="PLF67_04390"
     CDS             19437..19874
                     /gene="fabZ"
                     /locus_tag="PLF67_04390"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOE89287.1"
                     /translation="MNIVKLSPQEILPHRDPFLFVDEILEADESHTVAKRVFTPDHFF
                     FKGHFPDYPVVPGVLLIETMAQAGGAGLGQAGIIPLGSLFVLATIEKAKFRSQVRPGD
                     EAVITVENVRISQMVLRQKGTITVNGTVAAEASWMCILVKGEE"
     gene            19876..21057
                     /locus_tag="PLF67_04395"
     CDS             19876..21057
                     /locus_tag="PLF67_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HOE89288.1"
                     /translation="MVITRKVLRNVSLTAHPLGCRRHVEDQIVWVKNPKGSLYSPVPE
                     KMPARVLVIGGSTGYGLSSRIVAAFGGGADTINVSFEREPGEKKTATPGWYNTKVFEE
                     HARKSNLRAFSVFGDAFSDEVKERTADLIEKEFGQVDLIVYSLASPVRIDPKTGATYR
                     SVLKPIGKPYSALSVNLDDPTITKETIEPATAEQTEETVKVMGGEDWKLWIDLLDGRN
                     LLAEGAVTVAFSYIGPEMTYPVYREGTIGKAKEDLEKTAGVLSKTLRAYVSVNKALVT
                     RASAVIPVVPLYMALLYAVMSEQGIHEQCTEQIWRLFTDRLYAGTPVPTDKAGRIRLD
                     DWEMDPLIQAAVQRRWDMQREGKPLKGGNLALVRQEYDRIHGFGYKEIDYEADVDPRV
                     V"
     gene            complement(21024..22370)
                     /locus_tag="PLF67_04400"
     CDS             complement(21024..22370)
                     /locus_tag="PLF67_04400"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOE89289.1"
                     /translation="MVSTIVTKRDGREIQRVQKVEGFTWEALRPQAPPFDSRAIGEIA
                     RLYQVTIIPKNPGKFGWFVFFRLDEDLPFPLAMTQGEITFLDPNLAFASYVNGLVSRG
                     EIAVNDRLVVNDPVLREYLDRLAVKGLLSIAKGTADSLRFMPISDELGFASSVEGDLV
                     VNSHFFLMDSSDLDSPFCTLGSPYGLSVHQGLVELPPLNERPALFASKAGETWIGVPK
                     LSDLTIEVDGIPLKGTIHTRREGRSTPPSDNQEIVIVGRKVVGVATGGKTPIPMAGFV
                     VSCKEAIEPGKGIVTYKGYEDILFAIQVGPSMMNAGEIVDSFDCFFYDPKCNDTAFPP
                     TVYPLDFNTGRAARIALGTNNEGKPILLWAEGASKVRHVPGAGSSGASLLELARFCEE
                     EGYGNIVNLDGGGSAQILIDGKRQLKISDRHPSDEEAERPVPRVLVYTTRGSTSAS"
     gene            complement(22352..22972)
                     /locus_tag="PLF67_04405"
     CDS             complement(22352..22972)
                     /locus_tag="PLF67_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOE89290.1"
                     /translation="MKKPVTLLALDFGGVVVEFIDDEVLEAMSKHTGFDHPTFTERYW
                     RHRSDFDRDRYTLLEYWRLVTGEENPPIDALVSLDLAGWSRSDPAIIRWAEGLKERGL
                     KLALLSNMATPTYEALQKDEHWPTFFDYYIISGVLKVNKPDRAIYEHLIAVSGTKPSE
                     ILFIDDLMHNVQAAQAAGIHAIHYRGMDDLATILRSEHPSLWYPRS"
     gene            complement(22974..24323)
                     /locus_tag="PLF67_04410"
     CDS             complement(22974..24323)
                     /locus_tag="PLF67_04410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE89291.1"
                     /translation="MIRLTKGVFYDLAIWMMGFGMLVGVVFPFFMEFLGMDPSTVYTV
                     WFFSVCIFSGLLVGAVNILLAHWVVGSRLNMLSTHMGKIEDKLHMISGSDTMVDCSAQ
                     DCHLLVDSTDAIGRSSASFNSLVDTLSSSLQLEMQIRSYTRMLTTHLEVEALCQNALS
                     SLLELFNIDGGAVLVEIKGNLKLLGSSGLANGEELGKNPQVLDTLSQLNRKRIDIPVQ
                     AEIDGVIARFRPQTVLLFPIIYKGLGMGVVILAAKHSIPRDVEKNLDLFISSLALALH
                     NAVTHDQIQRLAAVDPLTGVYNRRFGLTRLREEFIRSVKQNTTLGILMIDVDHFKDVN
                     DTYGHTVGDRVLQGIASSARTQLREGDLLIRLGGDEFMVVLLGASGNDVLEVGEAIRR
                     TVSERTTSWGEQQIKVTVSIGGASLATANVKDEQDLIDAADKALYQVKESGRNRVGI"
     gene            complement(24320..24901)
                     /locus_tag="PLF67_04415"
     CDS             complement(24320..24901)
                     /locus_tag="PLF67_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOE89292.1"
                     /translation="MMLYLQLFLSFFQIGLFSIGGGYVAMPLLQHQTVDLHGWLTQAQ
                     FIDLITIAEMVPGSMSINSATFIGIKIAGIPGAIVAIVGNILPSFIIMLILAKLYEHF
                     SEQRIIRNVLTSVRPIVVAAILTAALILLEFAVGTFKQPNIFAIIVFLLAYTLLSSKR
                     IPMGPVSVLGVGALVGVAKLGIVYLLEAGIVVI"
     gene            complement(24898..25509)
                     /locus_tag="PLF67_04420"
     CDS             complement(24898..25509)
                     /locus_tag="PLF67_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOE89293.1"
                     /translation="MKTEIKKGKPEVNVKFFLRLFWETFTLSMSGFGGGFVIVSLMRK
                     KMVAKLGWIDQDEMMDFVIIAQSAPGSASVNVAMMVGYHLARTPGALVATLGTVLPPM
                     IIVSIISVIYTILQGNVWVDAAFLGMRPVVAAIVLQVSWKFFKPFLKVKAIPAVLLFI
                     ISFVLLYILKVSMIALILVMLALSTTANLLDMRRRKKRMEEDI"
     gene            complement(25521..26609)
                     /locus_tag="PLF67_04425"
     CDS             complement(25521..26609)
                     /locus_tag="PLF67_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOE89294.1"
                     /translation="MREHLTMMIKPASAACNLACDYCFYLDTARNRSVHHHPVMSDEV
                     AGKIIDKSMALARNIQFVFQGGEPSLAGLSFFERFVSRVNERQGEGQRITWAFQTNGV
                     GLDRSWADFFKAHGFLVGISFDGTRRLHDLHRVDRQGLGSGRSVLNTIDLFTKEGVDF
                     NILSVVTNDLVDNVQMLYSFLNEHGLHYQQYIPCIDPMQGGKRFLSAEGYERFLKAMF
                     DLWFDSFMNQTPVSNRFFDNLVGMIAGYPPESCDMAGICSVQYVFEANGDVYPCDFYC
                     TDEHRLGTILFDEFSTLDERRRGMRFIEESSNTIDGCETCPWKHLCRGGCKRYRNDDG
                     YRFCSTMMGFFPYVIERLHYVAKQITPP"
     gene            complement(26806..27963)
                     /locus_tag="PLF67_04430"
     CDS             complement(26806..27963)
                     /locus_tag="PLF67_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE89295.1"
                     /translation="MILKLENNRAGIVKRMQSGYGCFIPHNLKDLTITIDDDMNRLIH
                     RAYLLLGRLDGMAITLPDIDLFVSMYVQKEAVISSQIEGTQASLVDVLQKDRKNKKTK
                     DTEEIVNYVKATNYAFKRLNELPLCMRLIKETHAVLLSNVRGEEKMPGEFRKSQNWIG
                     HVGSTLQNASFIPPEPSEMDICLSDLEKYMHEETAISNLIKIALIHYQFETIHPFLDG
                     NGRMGRLLIVLFLKERGLIEYPVLYLSYFFKKNRNTYYELLNNVRIKGHFEEWITFFI
                     EGICEISEDAIMSIQKIVELKKNDTEKIRSCSKGNLSNLLSVYDYLLKHPFIEADDIK
                     DLLDLSKPTIHKILDNLTELGILELVDEEKKRYRQYVYKKYVDILSEGTIL"
     gene            complement(28325..28867)
                     /locus_tag="PLF67_04435"
     CDS             complement(28325..28867)
                     /locus_tag="PLF67_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE89296.1"
                     /translation="MGSTTEKTMPETLFDQELLDKNPHLVVIKSISKSFGVPGLRLGV
                     LATTNGTIMKRISKEMSIWNINSFAEYFMQIWGKYRSYQGSLTLIKEARIQFEKELES
                     VENLTVFPSRANYILCEVHAPVSSHDLAVRLLVEEGLLIKDLSKKKGIAPRQCIRLAV
                     RTKEENTRLVEALKRHLGPL"
     gene            28854..29804
                     /locus_tag="PLF67_04440"
     CDS             28854..29804
                     /locus_tag="PLF67_04440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE89297.1"
                     /translation="MVLPTYRVAQFVERAIVSLQSQCFKAFEMIFVDDCGTDGSIDIV
                     ARHAERDPRIRIVKHERNRGTYHARRMGVEHARGRYIVFLDPDDELDEHCLSQLHEHL
                     HGSPASVIFYGVKTVPPPPWYKSSTRSFPVRSRERLLESMFKKSIGGYAFTTLGTPGK
                     AYDRKLLLAVYDELAIPGDFRYFFSEDSVVLFACAMKDPVFLSVEYDGYIYHLNPSAI
                     TSKKRMSLRSQHIFDQVAFTMDKLQHLINRHHLAKSERTFLHHYIHHNTKSELLLLKR
                     FDSSGARYFTSVASALFARPRLKHAAMLLFFVVTLGRKRL"
     gene            29889..30374
                     /locus_tag="PLF67_04445"
     CDS             29889..30374
                     /locus_tag="PLF67_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89298.1"
                     /translation="MSSKQEETKKVGKAQVIILSTFAVIIVALSVFGCYGCSYHPVSP
                     PTSEEALSIVERIRETVWELDEAGEVKTIGELGGKLKTIEILSSGVEGVSLDVLLEFE
                     NSMPKEGKLLFLEDSGFSLSESGIPLNVTIVFSNSKDGNSETLTLKGNESNSYLYYLK
                     I"
BASE COUNT         7275 a         8433 c         7966 g         6862 t
ORIGIN      
        1 ccgccgaaac ggcgacctgt tgggccggag tcaacgcccg gcagatccgg cccggaacgg
       61 ttcattgagg agcgacatca catcgcctac atctgccggc aatctcagtt cctgctcttc
      121 ttccaatctg tatgccggct tcgtcggcgc actcttgagt caatcgatga tatgtagctc
      181 cggaaactcc tatcttgata gcaatatatg ttttgtatgt acctttaccg gccccggtgg
      241 tgagtccccg gatcacgtac ttccggaaga tcatcgtcaa aatgatcgcc aaagtcataa
      301 tcatgactac ggcggccatc actgaacccc aatggacttc gacgcaggaa aggaggtggc
      361 gatcggacgg gttcgcatct tttctcattt caaaatacaa cgatgaagcg ccggattaat
      421 taacccaaac cctaagaagc cttacaatga caaagaaaaa gtgacatcac cggagacaca
      481 ttccgggaaa gattcaagga gaattcgtct cggactcgcc ataaagccag tcagctatgt
      541 cttttattat aattccttca tattgataag gttcgtggcg cgttctggcc aatatcattt
      601 tagggtaggc atcattaata gccagtaacg atgagtactc ccgctcaaat gtttccggat
      661 tatcgatgaa ttcggctact tgaatatata gtttctcgtt ttgcctgatg gcgacaaaat
      721 caatctcttt tttatagagc acacctgcat aaacttcgta tccgcgcctt aataactcta
      781 atgcaacgag attttccgac attctgccaa aatccgggtt tctgctaccg agcctggcat
      841 ttctaaatgc cggatcggca agataatatt tatcatttga cgctaaatat ttctttctct
      901 ttatgtcgta tcttctgata cggtaaaaag cgaacgcatg gcacagatga gaaatatatg
      961 cgctaaccgt tctgtcattt gttttaatcc tattcgaaat cagcgtgttt gccattttgt
     1021 ttgccgacac aagatttgaa acattgttca ttaaaaagtc cgttattttt tccagaagcg
     1081 gcagatttcg aattttatac ttttgaacta tatctcgaat tacaagcgta tcaaagacgt
     1141 cattcaaata tctggatctg ctcgcttcat catgatatac atatgacccg gacattccac
     1201 ctgtttttcg atattctata aatgcttcat agacatctga ttgttcaaaa taatccatat
     1261 actctcgtaa agagaatgga aacagttcaa tactgaatgt tctaccggta aacaatgtag
     1321 ccaaatcact gctgagcaga aacgcatttg aaccggtcac atatatatcg tatttttctg
     1381 acgcatgaag actgtttaat gcaagttcaa agccttcaca catttgcacc tcatcaatga
     1441 agagatagtt attcttcgaa ctgcaatagt gttcttccac gtatgaatta agagtctgat
     1501 aatgcttcaa ttcttcaaac tcaatatcgc tgtaatcaat gttgataata ttcgcattca
     1561 aatcaacttt tcgaatatat tctgcgtaag atgccattaa tttggatttg ccacagcgtc
     1621 gtattccggt aatgactttt atatccggaa cgccttttac tcttttcagt tgattcaaat
     1681 aaaaaggtcg ctcaatcagt ttcatcatat gctccccaaa gctttttcat atggtaccat
     1741 tctatttcga gatattcaat ttttttaagt ttctcgaaat agaacaacga tagaaatcgg
     1801 actgtaagag ccacaacgat tgtcgattcc acggatgaca tctcattgct ccctcagatt
     1861 gtcctgtcag ccggggtggt ctacatccga tggaagatta ttgccaaggt cataatcatg
     1921 gctacgacgg ccatccctga acccctatcg actccggcat aggcaagaaa gctatacaca
     1981 gcaaaaaact gaaagtattt ttcatatctg tgtttatttt cccatttttg aatttatttt
     2041 tctgaattag accgagtttt cccggttttt caggaagaaa tcaataaata tgaatatttt
     2101 tgccattgta tgaattttta tttcatacta tactgaaact atggcaaatg gcattcacga
     2161 catctccgcc ctcttcacga tcaaggagcg atacgatgat ttgagcgcga aagagaagca
     2221 aatcgctgat ttcatcctcg agcatccccg tgagtccgtg aatccttcca ttgaagaact
     2281 tgccgaacgg atcggaatca gcgaatcgac gatggtccgc ttcgcccgga agctcgggta
     2341 cagcggctat caacgcttcc gcatcgccct cgcccgggaa acggtccccg tgacgagtca
     2401 gctctttgag cgggaagtga tcgatgcctc ggacgggttt gacgccgtct tcaacagcgc
     2461 gattgcaagc ctgcttgaaa ctcagaaatc ccttgatcgg ggtttggtcc ttggagtcgc
     2521 cacgttgatg gcgaacgccc gaaacgtcta tctgatgggg ctcggcggat cgaacatcgt
     2581 cgcccaagac gccctgcaca aactggttcg gaccggaatc aactgccagt gggcggcgga
     2641 tttccacctt cagctgatga tggccagcca aggaacgaag gaagatgtcg cgctgttgat
     2701 cagtcatacc ggaagcggct atgactcgat cgccctcgct gaagagctca aagcgaatca
     2761 ggtgaagctc gtggtgctga caagcaaccc ccgctccccg ctcgcccggc tcgccgatta
     2821 catcctgacg gtcaaaacag gtgcttccgg ggcggtctcg gagtcctttt ccgcccggat
     2881 cgttgctttg gcgatcatcg atctcttgta catccacgtc atggagctcc tggatgaacg
     2941 ggggatcgaa caattgaaca gaatgcgcgc ctccatagcg aagcgccgcg tataacataa
     3001 ggagaaacgt acaatgaaac aagcacagat tggcttgatt ggattggcgg tcatgggcga
     3061 gaacctcgtc ctgaacctgg aatccaaagg cttcagtgtc gcggtctaca accgaaacac
     3121 cgaacgggtt gacaattttt tggcttcccg agccaaagga aaggcaatca tcggtgctca
     3181 cagcgttgaa gagcttgtga gcaaagtaca atcgccccga aaaatcatgc tgatgatcaa
     3241 ggcgggcgat ccggtcgatc agatgatcga atcgctgatt cccctgctcg aggcgggcga
     3301 tacgatcatc gacggaggga actccaactg ggaggacacc gagagacgag tcgcctatgt
     3361 ggagtccaaa ggcctgctct acatcggaac gggcgtttcc ggcggtgagg agggcgccct
     3421 gctcggcccc tcgatcatgc ccggcggttc gaagaaagcc tggcccgccc tcaagccgat
     3481 gcttcaaggg atcagcgccc atgtcgacgg gattccctgc tgcgactggg tcggcagcgg
     3541 cggagccggt cacttcgtga agatggttca caacggcatc gaatacggtg acatgcagct
     3601 gatctgcgag gtgtatgaca tccagcgccg gctcttgggc ctttccaaca aagagatgca
     3661 caagatcttc gccgagtgga acgagggcga gctcgattcc tatctgatcg agatcaccaa
     3721 ggatatcctt gcctatcagg aagatgacgg cactttcctc gtcgacaaga tcctcgatgc
     3781 cgccggccag aagggaaccg gaaagtggac cgggatcagc gccctccatg aaggcgttcc
     3841 cctgaccctg atcgtcgagt cggtgttcgc ccgcagcgtc tccgcattga aggagcagcg
     3901 agtggcggcg agtaaggtct tcgatcttga gatcaaggct cccgcctttg acaaaaagca
     3961 ggtcatcacc cagcttcagg aggcgctctt cgccgcgaag atcatctcct acgcccaggg
     4021 cttcgacctg atcaagaccg ccggcatcac caacaagtgg gacctcgacc ttggcggaat
     4081 cgcccagctg tggcggggcg ggtgcatcat tcgaagccgc ttcctcggca agatcaccga
     4141 ggcgtatcga aagaaccccg atcttcccaa cctgattctc gacgcccact tcgccgagat
     4201 tctcaagaag aaccaccaaa gcctgcgggc aatcgtcgcc ctggccgccc aaagtggaat
     4261 tccgaccccg tcgctgagtg ctgcactctc ctggtttgac agctaccgca ccgagcggct
     4321 cccggcaaac ctcttgcagg cgcagcgcga ctacttcgga gcccacacct acgagcggac
     4381 cgacaagccg cggggcgagt tcttccacac caactggacc ggccgcggtg gttcgaccgc
     4441 ttcaaccatt tactcaatct aggaggatac ttatgaaaac attactcgca aaagacaaag
     4501 cagcccgtga tctcctttcc ctcggtgcac tggttattcg actcgacccg ggaacgactc
     4561 cctttgaata cgcctcgtcc ttcgacctgc acgtctccgg aggagagttc aacgtcgcgg
     4621 cgaacctcag ccgggcgttc ggccagcgga cggcaatcgc cacggcgatg gtcgactacc
     4681 ccctcggtct gaagatcgaa gcggaagtcc gcaggatggg cgtccacccg ttctacaagc
     4741 gcttcgagca cgacggtgtc cggggaccca acatggccca cgtctacagc gatcggggcc
     4801 agggaatgcg cgcaccggtg gtcttctaca accggtgcga cgaagcggct gcccgcctgg
     4861 atgccgacag cttcgattat gacaagctgt ttgaaggcgg agtccgctgg gttcacagcg
     4921 gtggaatctt cgccgctctc tccaagacga cccccgagct gatcaaggag ttcttccgcc
     4981 gggcgaaagc gcagggagca ttcgtctcct tcgacctcaa ctaccgccag aagctctggt
     5041 cctcgctcag cgacgaaccc cagaaggcgg cccagaagac gttcagcgag atcgtccagt
     5101 acgtcgatgt cctcgtcggc aacgaagagg acctgcagat gggtctcgga ctgaaggggc
     5161 ccgaggtcca ctcgacggac aagctcgatc ctacggcgtt ctggggcatg gctgaacaag
     5221 ccaagaagaa gttcccgaac ctgaaggcgg tcggaacaac gatgcgggtc gtccattcga
     5281 ccaaccgcca cgactggtcg gcggtcctct atcttgacgg aaagggctac caggccccga
     5341 cggctcaact ggacatcctt gaccgggtcg gcggcggcga cggattcgcc tccggtctga
     5401 tctacggcct gcttgacggc cgcgagccgc aggaggcgtt gaacctcggc tgggcgcatg
     5461 gtgcactgct caccacatac cccggcgaca ccacgatggc gaggcttcac gaggttgaag
     5521 cgttcgcttc aggcggcagc gcccgaattc agaggtaagc catgaacgct ttggtattga
     5581 ctgagtataa gaatcttagc tatcagaagg taccaaaacc ggtcatcacc tccccgaccc
     5641 agacgttggt ccgaatcaaa gccgcctcga tctgcggcag cgacgtccac ggctacgatg
     5701 gctccaccgg tcgaaggcaa ccgcctttga tcatgggcca tgagggttcg ggggttattg
     5761 aagaagtcgg gtcgatggtc acgaacttca aggtcggcga ccgcgttacc ttcgactcga
     5821 cgatcttctg cggcgcgtgc cccttctgca agcagggact cttcaacctc tgcgacaacc
     5881 ggagggtgct cggagtggga tgcgacgagt acaagcagga cggaatcttc gccgagtacg
     5941 cgatcatcga agagcgggtg ctcttccacc tccctgaagg cctttccttc gaggaggcgg
     6001 cgatggtcga gccggcggga gtcgccgccc acgctctttc cctcgcctcc ccgtcgcttc
     6061 atgacgacgt ggcggtcgtc ggggcggggc tcatcgggct gcttgtgatc aagctgcttc
     6121 ggacggtgac cagcggaaag atcatcgccc tcgacatcga tgagaaacga cgggaaatgg
     6181 cgttgaaggt cggggcggat tacgccttcg actcgaccga tcccgccctg tcccggaaga
     6241 tcatgaagct taccggcgac ctcgggttga aagtcgtcta tgaagcggtc ggagccaccg
     6301 ctccgattca aacggcggtc tcccttgccc gcaagggcgg gactgtcgtc ctggtgggca
     6361 acgtctcgcc gaagatcgag cttccgctgc agagcgtggt aacccgccag atcaggctgc
     6421 agggctcgtg cgcgatcaac ggcgaatacc cggctgttct gaagatgatg gctgacaagg
     6481 ttctcgatgt gacggacctc atcagcaaaa ccgctccgct cagcgaaggg aagctttggt
     6541 ttgaccgcct ctacaacaaa gagggggacc tgctcaaagt ggtcctgatt ccataggagg
     6601 atgatcatgg ttcgatatgc attgctcgga tacggaaagg tcgcgcacct gcacgctcag
     6661 gcgctcaaga gtgtgaagga cgctcagctc gtcgccgtct gggggcgaaa catggaaaaa
     6721 gcccgacagt tcgcacagaa gtggaagatc aaagcgtata cggaccttga tcagatggtc
     6781 aaagaggcga aagtcgatgc ggtggtggtg accaccgtcc acccgcttca catggagcac
     6841 actcttcaag cccttgatgc cggccttcac gtcatggttg aaaaaccgat ggccctgacc
     6901 gtccggcagt gcgatctgat gatcgagaaa gcggaggaga agcacaagaa gctcggggtg
     6961 atcagccagc gccggtggta tccggcgtgc cagcggatcc gcaaagcgat cgacagcgga
     7021 aatctcggca ctccggtcat cgggcaggtg acgatgctcg gatggcggga caaggcgtat
     7081 tacgacagcg acccgtggcg aggatcgtgg gagctggagg gcggcggcgt cctgatcaac
     7141 caagcccccc accagctgga tcttcttcat tggtatcttg gggagatcga agaggtctac
     7201 gcccagtggg acaacttcaa ccacccctac atcgaagtgg aggacagcgc ggtggcgacg
     7261 gtccgcttca aaagcggtgc gatctcctcg atcctcgttt ccaactcgca aaagccggga
     7321 atctacgcca aggtccacgt ccacggctcc aacgcgttct cggccggtgt ccagacggac
     7381 ggcggggcga tgttcgtggc tggacagagc acgatcaccg acccggcggt aaacgacctg
     7441 tggactatcg aaggagaaca gggtaatctc gaaagatgga aagaagagga cgccaagctc
     7501 tttcgtgaag tggatgcgac cacctacttc ttcaccctgc agcttgagga cttcacccaa
     7561 gcgatcctgg aagacaggga tcccgcttcc tcgggatatg acggtcggga gacggtgaag
     7621 ctgatcgaag ggatctacca gagcggccgc accggaaagc cggtccgcta ttaaaggagc
     7681 gagcaatgca tcaggaactt caggagatcg tatacgaagc caatatggcc ttgaagcgca
     7741 acggtctcat caccctcacc tggggaaacg tctcgatggt tgattccaaa cgggaaatga
     7801 tggcgatcaa gccttcgggc gttccgtatg aatcgctgcg cccgagcgac atcgtgcttc
     7861 ttaaccttaa aaccaaagaa caggtaaaag gatctttgaa gccctcctcc gatactccga
     7921 cccacctcta tctctacgac caccttcccg aggtgggcgc agtggtccac acccactcac
     7981 ggtgggctac gatcttcgcc cagagcggga aagcgattcc cgccttcgga accacccatg
     8041 ccgatacgtt ttacggcccg gttccctgca cccggccgct gaacagggaa gagactgaag
     8101 accactatga gctgaacacc ggcaaggtga tcgtcgagac ggtcgaccgt tccaacccct
     8161 tggcgattcc ggcgatcctc atcgaaggcc acggcccctt cgcctggggc aaggacgcaa
     8221 aggatgcggt cgaacatgcg atcatcttgg aagaggtggc aatgatggcg taccacaccc
     8281 tccttttgaa agggaacgac catccgatcg accagcacct tctggacaag cactaccagc
     8341 gcaaacacgg aaagaacgcc tactacggtc agtaatatca acccgatcgg gtataattca
     8401 atctaaatca acccggtcgg gtataatcaa ataaaaattt gagtaaatca tcccgttcgg
     8461 gtatagataa accgaaacct tgaaaaaaca tcccgttcgg gtatactatc aagtatgaat
     8521 gaattatcaa ctcttgtccg cgaatatcgt aaacaagcca agctcactca ggaagaattt
     8581 gccctcttat ccggactcgg tatccgattc gtcagagagc ttgaaggcgg caagcccacc
     8641 gtcaggttgg ataaagtcaa tcaggcgttg gccttgctgg gattcaaaat ggctccggtg
     8701 ccaattcagc taaaggagtc ttgacgtggc atatcgcaaa gtactcgtcc ttgttcacaa
     8761 cattcgagcg ggaatccttg aagagacgga tgacggatac accttttcct atgatgacga
     8821 atacctcgca agacccgatg cgaagcacgt cagcctgacc cttcccctct cttcgactcc
     8881 gtatcacagc acgacgatgt tcccattctt tgatggtctc attccggagg gctggcttct
     8941 cttggtggcg cttgacaact ggaaactgga ctcaaaggat cggatgggac ttctcaacgt
     9001 cgcatgccga gatacgattg gcgcagtcac cttggagaac atggaatgac ccgctgtctg
     9061 tattgcaaca aaccgatttc acgtgatgaa cacgatttcc aatggcatcc ggcatgtgta
     9121 aagaaattct ttggaacaac gagcctgccg acgctggcgc ttgatgagag tcgacttctt
     9181 cacagtgccg cgacttctat cgagcagggg catgccgtcc cgggtgttca aaagaaactt
     9241 gctttgcatc tcttaaaaac ccggggcaaa gggcagctga ccgtcatcgg ccgtccgctc
     9301 ggctacatcg tcaagctccc ggtgaaggaa tacccgcttc ttcccgaata tgagcacctg
     9361 acgatgcaac ttgcatccct ggcgaggatt cccaccgtcg aacacgccct ctaccgcctt
     9421 gaagacggaa cgcttgtcta tctgacgaaa cgaatcgacc gggtcggcga gacgaagatc
     9481 gcgatggagg atttctgtca gctgagtggg cggttgaccg aggacaagta ccactcctcc
     9541 tgcgagcagc tggggaagat cctcaggcgc tactccaggc gcctgctcga tgcggtcgcc
     9601 ctctggcatc tcttgctctt ctgcttcgta acgggcaaca gcgacatgca cctgaagaac
     9661 ttctccctct acgctccgaa cggtgatgaa tacatattga ctcccgcata tgacctcttg
     9721 ccgaccaatc tcatccttcc tgaagaccgg gaagaaagcg ccctgacgct gcgaggcaag
     9781 aagaacaggc tgaaaaagga agattttgtc tgtctggcac agcagtatgg gatccactcc
     9841 aaaaccgtcg aaagactcat cgcccgaatc gtcggtcttc aaaagacatt cttcgaagcg
     9901 atcgagcaga gctttctgcc aaaagaggct caaaaagcgt ataaggcgtt gatagcggag
     9961 cggatcgggc gcttgtcata aacgtcgcct gaacatgtgt taccgtaacg ccataatggc
    10021 ctcccagaca cccggtgtaa cgggaattcc atccttggta ttgatccggc gcgttgcgat
    10081 cgtccgctcg ccgggataga agacgggaat atccggatca atcggttccg accgtttcag
    10141 atcgtcgatg attccttcaa ggattctttc ggcctcctct ctggttccca atcgataggg
    10201 atcgatcgcg atgaaggtct gggagatcgc gatctcgtcc tcacagagct ttccgatcgc
    10261 ctgggtggag tttcccccgg agaggacgga ggtgaccgca tcgaggacga gagccagaga
    10321 actccccttc caatacccga tcggcaggac cctgcccgtc tcctcgatcg ccttggggtc
    10381 ggtggtgagg tttccatccg agtcataccc tcccggatac ggaagctgtc tgtctgccag
    10441 cctggcctct tcaatctttc cataggagta ttgggccatc gccatatcca cgacgatgtg
    10501 ctttccattc tctcggggga tcccaagaac gaagggattg tttccaatcc gcccattcct
    10561 tgctccccaa gcgggcatgt tcggcagcgt gttggtgaag cacatgccga tacagccggc
    10621 ttcagcggct tgccacccat aggttccgcc ccgcatccag tggttggtgt ttcggaccgc
    10681 cacgcacccc atcccatgaa aagcggccaa ttcgatcgcc cgcttcatcg ctttcttcgc
    10741 agtcaggttc cccatcccga ggttggcgtt ccagcgctca atgcatccga aggaaccttc
    10801 aacctcgatc gaggcgttgg ggtcgatgta cccctttttg aggtattcga ccacccgggg
    10861 aaatcggttc accccgtggg aatacacccc gtccaagctg ttctgcacaa agatggcggc
    10921 gctctcagct gcatcggaat cggaaaaccc atgactgagc aggatcgtct tgaagacatc
    10981 ctgcattgtt tctttgctga tcttcatgga atccatcata ccacatcgga aaaacatcga
    11041 ctcccatcaa atcgatgaga gccaagaggc gatatcatcc tcaattatag tgatataatg
    11101 catggcttta gaccgactcc tttttgcacg ggcatagtct gttgattgta ttgactattg
    11161 accatcaatc aaatcatttt tgcaccgacc tgatgcaaaa attgatattt gtaggagaga
    11221 atgaatgcac gaatcaactt ctccttctat acctctgatt tctgctgttc ggcttatcgg
    11281 gaattgtgcg ttcaatgagt tcttgctcca aagccggatt cagatagttt ttacggaagg
    11341 tcggtctgtg ggagagtccg agccgatcca taagttctgc tgccgaaagg gtatcgtctc
    11401 caagaacaga cagaagacgt gcagtcggtg ttttatcttg gtcggtcact tggtcgctat
    11461 cttggtcggt ggagcgacca acaaccgtca cttcctttaa gctgtctcgg ataatctcaa
    11521 gcatcaattc cacaaagccg gcactatctg cttgcctatc agctgctccg agggcatcat
    11581 agtattcctt ctgcctcgac tggataagtt tttcgattgg cagccagaag aatagctctt
    11641 tccacttgcc gagcagaagg ctgtgccaca tccgtcccat acgtccattg ccgtctgcga
    11701 aaggatggat gaattcaaat tcatagtgga acacagcact tttgataagt ggatgaagct
    11761 ccgactgttg ataccaagaa aacaagttct ttatatgctc cggcacaaac tcagccggag
    11821 gagcgatatg aatcagcacc tcgccgtcaa acacacctac gccgccggag cggaattttc
    11881 cgttctccgg cacgagcccg ttcatcatca gcttatgagc cttcaataaa tcgtcaacag
    11941 atgccggatt cagacgcaac ataagttcgt aggcttcata agcgttctgc acctctttga
    12001 tctcattggg atttccaagt acacgcttac cgtccaagat agccgttacc tgctcaagag
    12061 agagggaatt gtgctcaatc gcaagggaag agtgaatcgt gcggatacga ttttcacgcc
    12121 gaagatgcgg gctgacagtt ccttcctgta aaacagtaat acgaccgacc tgttcactga
    12181 tttccgcaat cagcgatgtc atgttatccg tcatatgaaa aggcggttta tagtcgctca
    12241 taggcggtta cctccaaatt acatttctat gtctacaatg gttaaagttt tacatgccat
    12301 tatagttatc ccatcgccat cgaagaaaat ccctgtttgt gaaaggtgga attacaacct
    12361 taccttcctg aaatgttatt actcaaaatg aaaacacctg gtcaacgctt tccgctcttt
    12421 tcgattgcag ttatcagctc cttcgcctga agatttttct tctcaagttc tggtattgta
    12481 gcatcatatc tggctttcgt cttttcaaca gtcgtctgag ccttttcaat ctttgcatca
    12541 atggaagcca tcgtcttagt tcttgccata cttcttgcct ccggattgtg cagcaagcgt
    12601 ttcgctgatg gacactgctg tagtgtgaac atcatcactg tccagcccaa atgccttcag
    12661 aatcgtcttc tgagtcttgg ttaccgcatg atcgagtcga tacactccat catattgcct
    12721 caccatctcg attttgtcca attctttcaa tgcggcagga accgtcatgt agtttggcct
    12781 cttgtccata cgttccgtct catccttgag aagggtgtag agacggctac ggatgatgaa
    12841 ctcatagccg caggaatcca tgtaccggat attcggcttg cagaagtatc cgcggtctag
    12901 aatgaagccg cagtgtcggt agccgtagct tttggccttc tggagcatat actgaagctg
    12961 tgagatgtca acaatactgc cgggatatgc ttcataaaag agcggaacct catcatcgca
    13021 ggattcaaag aaaccagcga tacggctttt tcgaccgagg cgcaggtgct gaccggcgaa
    13081 gtgaacacgg tcgcagaact gatacagaag tgcatagacg aaaatgcccg cgtttcttct
    13141 cttattcctt tttggaaatc gcgttgagaa tcacgatgac gacgtagatg agaatgatca
    13201 cggtgaagat tcccaccatc cctttgagca tcaagaccaa ggattgctga aatatcgaat
    13261 tcatcccacc ctcctacagc atcgccggca caaggccgag aatgacgccg ccggcgacga
    13321 cgctggcgat ctgaccggcg acgttcgccc cgactgcatg catcaggagg tggttctggg
    13381 gatccgcctc ctggcccatc cgctggatta cccgggcgga catcggaaac gcactgatcc
    13441 ccgccgctcc gagcatcggg ttgattttct tctttgtaaa caggttcagg aacttggcga
    13501 acaggacgcc tccgatcgta tcaaagacga atgcaagcag cccgagcagc atgatcatca
    13561 gcgtctgaag gcggacgaac tgctccgctt tcatcgtcga tgcgatcgtg attcccagaa
    13621 gcagggtgat gaggttcacc agggatgtct gggcggtctc cgaaagcgag tccagaacgg
    13681 tggattcccg gatcaggttt ccgaacatca acagccccac cagagagacc gaggcgggcg
    13741 ccacataccc ggcaatgatc gtgacgacga tcgggaagat gatcttcgtt gtcttggaga
    13801 cggacttggg attgtagctc atccggatca tccgctcttt ctttgtggtg acaagcttga
    13861 tcgcgaaggg ctggatgatc ggaaccagcg ccatgtagga atacgccgcg atcgcgattg
    13921 gaccgacata cccggacttg agcacctgcg agacgagaat cgaggtcggt ccgtccgccg
    13981 ccccgatgat gccgattgag gcggcatccg ccagagagaa gcccatcagc gtcgccacgc
    14041 tgatcgtggc gaagattccc cactgcgccg ccgctccgaa gagaatcagc ttggggttcg
    14101 acaacagcgg tccgaagtcg atcatcgccc cgattcccac gaagaggaga agcggcatca
    14161 tctcggccga ctcgatgccg acggagaaga gccaatcaag gatcccctgc accggaccga
    14221 tcccttcgac aagctgcgtc accgccccgc tgaagggaag gtttacgaga atcgctccga
    14281 agcccatcgg cagaagcagc gagggttcca gttttttcct gatggccaaa tagatgagga
    14341 ttcccccaac acagtacatg accagatctt tccacgtcgt ggccagaata ccgcccaata
    14401 agaagtccat aaagacctcc ggggtaataa aaatagggga agaagttgac ttcgcccaac
    14461 ggacaggaag cttccccgac gagatcagtg taatggtcta tgcagttcta gtcaaacagc
    14521 agcctacacc tcttttgcga tcgtctccag ggcgacgacc accgacgggt agaagcgttc
    14581 ttcaggcagc agggaataga agtccagccg ccttaacatc gactccaccc gcggctgaac
    14641 cccgcagaag agcagctcga cctctttctt ccgggcgagc cggacgatct cggccagctc
    14701 ggtgatcgag ctgtgatcga tattgggaac cccccgaagg gatagaagaa tccgcttgac
    14761 cccgctctca atcagcggag cgatcgattc ggtgagcttg ccttgcgagc cgaagaagag
    14821 cgcaccatcc acgtacacca ccttggtggg tttcgctttc ttgccaagcc ggttcgtaac
    14881 cggatcgatt tcgatcgtga tcttgttgct cttgatgacg aacatcacga ttgaaaaacc
    14941 gattccaagg aggatcgcgg tggtgagatc gaagacgacc gtggcgacca gggtgatgga
    15001 gaattgggcg atcgaggtct tcatccgctt ggagaagatc tgcttgatcg atttccagtc
    15061 attcatccgc catgcggtca ccaaaagcac gcccgccaac gcggcaagcg gaacccggct
    15121 taagaacgga gccagaaaga acacgcttgc gatcagggcg atcgagtgga agatgctggt
    15181 aagccgggtc tgctggcccg cccggatcgc caccgacgta cgggcgatcg cggcggtggc
    15241 gggaatgcct ccgaaaagcg ggatgatcat gtttccgatt ccctgcgcat acagctcccg
    15301 cgtcgcatgg aacttctcgc ccttcatctt ctcggcgtac gttccgcaca gaaggctttc
    15361 gaccatcccg agcgcggcga ttgaaatcga cggcgagatg tagaccccca ggttcttcca
    15421 cggaagcgcg aagaggttga ggcgctgttc accgatgatg ctccggggaa tatgcccgac
    15481 ttcggcgacg ggaaggtcca gaatgatctg ggcgatcatc gtgacgatca gcgccaaaag
    15541 cgtggaagga aggcgttcat tgagtttctt gggccacagg agcatcaagg cgatgacgaa
    15601 cccgccgaag agcaccgaga gccaatcgat gttgaagccg aggcgggtat aggagaagag
    15661 tcgttccaga atcgtgacgc cttcgcttac ggtcccgaag aaggaattga tctggccggt
    15721 ggcgatgatg atcgccactc cggcggtgaa gccggtgaca acgggaatcg ggatatagga
    15781 aacaagcgct ccgaccttca agaccccggc aaggatccgg atgattccgg cgaggaacgt
    15841 aacaataaga accccctcat gcccatgatt cgccacgacg atcagaagga ctcccgccat
    15901 cgttccggta ggcccggaaa tctggaacga cgctccgccg agggctccga tgatcaggcc
    15961 ggagaagatc gccgtgatga ttccgctggc ggcatccgct ccgcagctga cggcgaaggc
    16021 gagggcgagc ggcagggcta ctgccgctac cgtcaagccc gccacgatat ctttagcgag
    16081 cttgcccccg ttatagccgg caaactcttt ttgcagatcg ctgatgaatg taccttgttt
    16141 ttttcctgcc atccccccac cctccgcctc gtcggccata tactgcaagc atatctgata
    16201 gcggggcggt tgctcaaggt cgaagtcgga gtaggcacat ccgcccctca tacaatcctc
    16261 catcgctgac cgtttcgttg ggcggccagg acctcaacat catatgacga taccgcctcc
    16321 ttcaaagccg ttccaaccgc aaactcgccc cacatgtgca tcgggaccaa gtaggagacg
    16381 gagacccgtt caagcagatc gcgagcccct aacccatacc gcttcctcag gcgcggatcg
    16441 acgggaacga acgcgaggtc gaccgcttcc ggtagccgat cgatctcttt gtgatactcc
    16501 cgttccatcc atgaggtata cgcttctccc tcctcctccc agaaccagtt gttcagatcg
    16561 ccggcgaagt agatgacctt tccttccacc tcaagcagga aggcgacccc ctcgtcggtg
    16621 gattgaagag tccgtgcgac gaggccttca acctcgatcc gctgatggga acccatccga
    16681 tggacgacga ttccatgggg aatatcttcg gtgggaatat cgtcgctgag aacgaacgct
    16741 ttggcgggaa gatcgaagat ctttttattg taatgatcgc cgtgccggtg ggaagcgaag
    16801 acgatgaggt ccttgttttc cggaagcgtc acctctccat cgaaatagtc gaagaggagg
    16861 gcgaccttgt cgcattcgac gagaaaggaa gcgtgctgaa gaaacgtgac aaccatgcct
    16921 acacgatgaa agagaagaaa gaaggtgtca agcgcttaca tcccctccag catcgtcagc
    16981 gccgtctcaa gaagacccag ctcattgtag tagtcgagca ctcccaagtg agcgctgcgg
    17041 agcatcatcc tgagcagctc gtccttaagt tcgccgagcg aacaccgcgc ttcctcgacc
    17101 gagacagcgc acccggcctc cttcagcttc ctcttgatct ccccgaaggt gaggatattc
    17161 gcttcaaggg tctgtttcac ctgattcagg gaggggcgcc gttggaagga cttcggcttc
    17221 ttcgcaagga gcgcttcctg ctcggcaggg agaaagaggc gtttgatcag gtcttcccga
    17281 tcttttttca tgtgcccgac cgcatcccgt tcactgagcg tgaacagctg ctcgccgaga
    17341 tagaagatcc ccagcgccag aagggaaacg gggacccttc ggttccgcct cgtgacatac
    17401 tcaagctcct gatcgaccac cccgccctga gccagcgctt tcgcaagaag ctcgaggctg
    17461 tacctgctgt tgtccaggta cgcttcgaag aactcgtcgg cgcttcccca tgccagagta
    17521 tgcagatcga tcctcaggtt ctcctcgccg aacagcaccg cctccccgtg ggttgcaaga
    17581 agcagcaagg cctcggcgac cgcttgaggc aggatcttcg gctttctgat cagttcggtg
    17641 tcgacgagga ggagcgtcgg aggctgggag ggaatgaacg cccccggcca gatgaagcgc
    17701 cagggactgg cgatcgccgg tgaatcgaga accgtgggaa tcgcggcgaa ggagcgattg
    17761 agcttgtaag agacccgctt ccccaccgaa aggaggcggt catccccgaa aaagacgggc
    17821 accaccggtt tatcatccaa aagaggaaat atcttgttga acgcggctcg cttgaacgta
    17881 tggccggttg cctccacccc ctctttttga agggagcgga gaatggggag ggcgtagtgg
    17941 tgatgttcat gctcttcggc gatgatcatc gcctgagcgt gcccgaaaca ctcgatatat
    18001 ccttcggcaa acttctgaag ggcgttttta ttaatgatga tgacctttgt ttccataaga
    18061 catcgcctcc ttcttcatca agaggtgcgt tttttcaaga gcgttcgcac tgctcatccg
    18121 ggcaagaatc ttcttcatca aggcaaacag cccgccttgc ggggtgtagt ccgcttcgat
    18181 gaagaaattg atccgcccgg cggccatcag gcggtggcag aggtcgacct cgcctttggc
    18241 gtgaacggcg atcgagatac ccccggattc cttcaccagc ttcatgcacg gtacgtcggt
    18301 gagtccgtcc ccgatgtaga tcatgttgac aaagggaacc gggcgcagct cctgcggtgt
    18361 ataatcgttg agcgcattgt cctcccacac atcaagaatc cctttgttga ttcgaaagag
    18421 aaactgcgtc tttgctgtat agttgatcgc cagcttcggc cacttggcga ccccgttcac
    18481 gtcgtagtag tactcgcatg cgtagatctt tttaaagtgg ctgccgatcg ccgaaccttc
    18541 gatgatctcg gaaagtccgc ttgagatgat gaagtgctcg atgtggagcc ccagttccct
    18601 tccaagctcg ttgacatggt cgaaccacgc ttcgaccccc gggtagaact ccagcgtctt
    18661 ccccaacgag acgaagtccc cccggcgaat cggctggtcg tgccgtttcg cctcatccag
    18721 catcagcttc atataggcga ggaccgagtc catcccctcc ttccgggcaa gctcgttcgc
    18781 ctttccccag aaggcggcgg cttccatccc cagattggga atgaaggagt actcctgcat
    18841 cgccctggtc gtcagcgtct tgtcaaaatc atacatgatc gcaatcgtcg gccggttgtc
    18901 catgtaaaaa gagtataccc ggggaagctc acggggcaag agcaaacggt tgtcaactgc
    18961 ttcctttgta taacacggcc atgcaggata tgctccaaac tccagcaacc gccgaggtgg
    19021 aggaactcac cccatgagtc ccgctgcagc ctccggcgat cgtcggaatg gtcgtcaaca
    19081 cgccgtacaa cgtggtgggt tctcaagatt caacctgcaa accctgagaa actatatttg
    19141 aagtatccaa tatgccagac actgtccggc acattgagcc ggtcacagtt ttgtgagcta
    19201 ttatctattt ccgacgatac tgatccgaag agcggtctct tacgttgaac tccttgaggt
    19261 ggcggcttgt catctttatc gcttccgccg acgatcaacc tcgacgagcc ggatgaggga
    19321 atggatttgg actatgtacc gaaccatggc cgaagtggta ggatacgcta tgcgatgagc
    19381 aactccctcg gcttcggagg ccacaacggc gtgcttgtct ttaccacata caaggagtga
    19441 atatagtgaa actgagtcct caagagatat tgccccaccg cgatccgttc ctcttcgtcg
    19501 acgaaatcct cgaagcggat gagtcccaca cggtggcaaa gcgggtcttc acccccgatc
    19561 atttcttctt caaagggcat ttccccgact acccggtcgt ccccggagtg ctcctcatcg
    19621 agacgatggc ccaagcgggc ggagcggggc tgggccaagc gggaatcatt cccctgggct
    19681 ccctcttcgt cctcgccaca atcgaaaagg cgaagttccg ctcgcaggta agaccggggg
    19741 acgaggcggt catcaccgtc gaaaacgtcc ggatctctca gatggttctt cgccagaagg
    19801 ggacgatcac ggtcaacgga accgtcgccg ccgaggcgtc ctggatgtgc atcctcgtca
    19861 aaggagagga atgagatggt catcaccaga aaagtgctga gaaacgtttc gctgaccgcc
    19921 caccccttgg ggtgccgccg ccatgtcgag gatcagatcg tctgggtcaa gaacccgaaa
    19981 ggatctttgt actcccccgt tccggaaaag atgcccgccc gggtgctcgt catcgggggg
    20041 tccaccggct acggcctctc ctcccggatc gtcgccgcct tcggaggcgg ggcggatacg
    20101 atcaacgtct ccttcgagcg ggaaccgggc gagaagaaga ccgccacccc gggttggtac
    20161 aacaccaagg tgtttgaaga gcacgcccga aaaagcaatc tgagggcgtt cagcgtcttc
    20221 ggcgatgcgt tcagcgacga agtgaaggag aggacggcgg atctcatcga gaaggagttc
    20281 ggacaggtcg acctcatcgt ctactcgctg gcaagtcccg tccgaatcga ccccaagacc
    20341 ggggcgacct accgctcggt tttaaaaccg atcggaaagc cctactccgc cttgtcggtg
    20401 aacctggacg accccacgat cacgaaggag acgatcgagc ccgctacagc cgagcaaacg
    20461 gaagagacgg tcaaggtgat ggggggcgag gactggaagc tgtggatcga tcttcttgac
    20521 ggaagaaacc tccttgccga aggggcggtc accgtcgcct tctcctacat cggaccggag
    20581 atgacctacc ccgtctaccg ggaagggacg atcgggaaag cgaaggaaga tctggaaaag
    20641 accgccgggg tgctttccaa gaccctgagg gcgtatgtct cggtgaacaa agcgctggtg
    20701 acccgcgcat cggcggtgat tcccgtcgtt ccgctctaca tggcgctcct ctatgcggtg
    20761 atgagtgagc agggcatcca cgagcagtgc accgagcaga tctggcggct tttcaccgac
    20821 cggctctatg ccggcacacc cgttccgacc gacaaagcag gcagaatccg cctcgatgac
    20881 tgggaaatgg atcccctaat ccaggcggcg gtgcaaagac gctgggacat gcaacgagaa
    20941 ggaaaacctc taaaaggagg gaatcttgcc ctcgtccggc aggagtatga ccggatccac
    21001 ggcttcgggt acaaggagat cgattacgag gcggatgtcg atccccgggt ggtatagacc
    21061 agcacccggg gaaccgggcg ctccgcctcc tcgtccgagg gatggcggtc ggagatcttc
    21121 agctgccgtt ttccatcgat caggatctgg gccgagcccc ctccgtccag gttgacgatg
    21181 ttcccatacc cttcctcttc acagaagcgg gcaagctcaa gcagggaagc gccgctgctg
    21241 cccgcccccg gcacatgcct gaccttgctc gccccttccg cccaaagcag gatcggcttg
    21301 ccttcgttgt tcgttccaag ggcgatccgg gcggcccggc cggtattgaa gtccagagga
    21361 tagacggtgg gagggaaggc tgtgtcattg cacttcggat cgtagaagaa gcaatcaaag
    21421 gaatcgacga tctcaccggc gttcatcatc gacgggccca cctggatcgc gaagaggata
    21481 tcctcatatc ctttgtaggt gacgatccct ttccccggtt caatcgcttc tttgcagctg
    21541 accacgaacc ccgccatggg gatgggggtc ttccctcccg tcgcaacccc gaccaccttc
    21601 ctgccgacaa tgacgatctc ctggttatcg gagggcggag tcgatcggcc ttcccttcgg
    21661 gtgtggatcg ttcctttaag gggaatcccg tcgacctcga tcgtcagatc ggaaagcttc
    21721 ggcaccccga tccatgtctc accggccttg cttgcgaaca gcgccggccg ttcattcagc
    21781 ggcggaagct ccacaagtcc ctgatgaacc gagagtccat agggagaacc gagggtgcag
    21841 aacggagaat caaggtcgga agagtccatc agaaagaagt gggagttcac cacaagatct
    21901 ccttctactg atgaagcgaa ccccagctcg tcggagatcg gcatgaagcg cagggaatcg
    21961 gccgttcctt ttgcaattga aagaaggcct ttgacagcca gacgatcgag atattccctc
    22021 aaaacagggt cattgaccac caaccgatcg ttgacggcga tttctcctct ggaaaccaat
    22081 ccattcacat atgaagcgaa tgcaaggttg ggatccaaaa aggtgatttc cccttgagtc
    22141 atggcaagag gaaacggaag gtcttcatcc aatcgaaaaa agacaaacca accaaacttg
    22201 ccggggttct ttggaatgat ggtgacctga tagagccggg cgatctcccc gatggctcgg
    22261 ctgtcaaacg gaggcgcttg aggccgcagc gcttcccatg taaagccttc aaccttctga
    22321 acgcgttgaa tctcccggcc gtcccgcttc gttacgatcg tggataccat aaagagggat
    22381 gctcgcttcg caggatggtt gcaaggtcat ccatgccccg gtagtggatc gcatggatgc
    22441 ccgccgcttg ggcggcttgg acattgtgca tgaggtcatc gataaacagg atctcggaag
    22501 gctttgtgcc actgacggca atgaggtgtt cgtagatcgc cctgtccggt ttgttgacct
    22561 tgagaacacc ggagatgatg taatagtcga agaaggtcgg ccaatgctca tccttttgca
    22621 gcgcttcata ggtcggagta gccatgttcg aaagaagggc cagcttcaag ccgcgctctt
    22681 tgaggccttc agcccaccgg atgatcgccg ggtcgctccg gctccatccg gccaagtcca
    22741 gcgagacaag cgcatcgatt ggcggattct cttccccggt caccaaccgc cagtattcga
    22801 ggagggtgta gcgatcgcgg tcgaagtcgg agcgatgcct ccaatagcgc tcggtgaagg
    22861 tcggatgatc gaacccggtg tgtttgctca tcgcctccag cacctcatca tcgatgaatt
    22921 cgacgacgac tccgccgaaa tcaagggcga gcagggtgac gggcttcttc acatcagatt
    22981 ccaactctgt ttcttccgct ctctttcacc tgatagagcg ccttgtcggc ggcgtcgatc
    23041 agatcctgtt catccttcac attggcggtc gccagggaag cgcctccgat cgagacggtg
    23101 accttgatct gctgctcgcc ccagctggtg gtccgctcgc tgaccgtccg tcgaatcgct
    23161 tcgccgacct ccagaacgtc gtttccgctg gcccccagca ggacgaccat gaattcatct
    23221 ccgccgagcc ggatcaacag gtcgccttcg cgaagctggg tgcgggcgct ggaggcgatc
    23281 ccctgcagga cccggtcgcc gaccgtgtgg ccgtaggtgt cgttgacatc cttgaagtgg
    23341 tccacgtcga tcatcaggat gcccagcgtg gtgttctgct tcaccgaccg gatgaactcc
    23401 tcgcggaggc gggtcagccc gaagcggcgg ttgtacaccc cggtcagggg atcgacggcg
    23461 gcgagccgtt ggatctgatc gtgggtaacc gcgttgtgca gggcaagagc gaggcttgag
    23521 atgaagaggt cgaggttctt ttccacatcg cgggggatcg aatgcttggc ggcaaggatg
    23581 acgactccca tccccaggcc tttgtagatg atgggaaaca gcagcaccgt ttgaggacgg
    23641 aaccttgcaa tgactccgtc aatctcggct tgaaccggga tgtcgatccg cttgcggttt
    23701 aactggctca gcgtatcgag aacctgaggg ttcttgccaa gctcctcacc gtttgccaat
    23761 ccgctggaac cgaggagttt caggttccct ttgatttcaa ccaagactgc cccgccgtcg
    23821 atgttgaaca gctcaagcag gctggaaagg gcgttctggc agagcgcttc cacttccagg
    23881 tgggtcgtga gcatccgggt gtaggagcga atctgcatct ccaattgaag ggatgaggaa
    23941 agcgtatcga ccaggctgtt gaaggatgcc gaagaacggc cgatcgcatc ggtgctgtcc
    24001 acgagaaggt ggcagtcctg ggcggaacag tccaccatcg tgtcgctgcc gctgatcata
    24061 tgcagcttgt cttcgatctt gcccatatgg gtggaaagca tattcagcct gctgccgacg
    24121 acccaatggg caaggaggat gttcaccgcc ccgacgagga gcccggaaaa aatacacaca
    24181 ctgaaaaacc agacggtgta cacggtcgag gggtccattc ccagaaattc catgaaaaag
    24241 ggaaagacga ctccgacaag catcccgaaa cccatcatcc agatggccag atcataaaac
    24301 acaccttttg tcagacgaat catattacta ctatacctgc ctctagaaga tatactatac
    24361 ctaacttggc gactccgacc agcgcaccga cccccaaaac ggagacaggg cccatcggaa
    24421 ttctttttga actgagcagc gtgtacgcca gcagaaagac gatgattgcg aagatgttgg
    24481 gttgtttgaa cgttccgacg gcaaactcca gaagaatcaa tgcggcggtg aggatcgctg
    24541 cgaccacgat cgggcgaaca ctcgtgagca cgttgcgaat gatccgctgc tcgctgaagt
    24601 gctcatacag cttggcgaga atcagcatga tgatgaagga gggcagaatg tttccgacga
    24661 tggcgacgat tgcgccggga atccccgcga tcttgattcc gatgaacgtg gccgagttga
    24721 tcgacatcga gccggggacc atctcggcga tcgtgatcag gtcgatgaac tgggcttggg
    24781 taagccagcc gtgaagatcg accgtctggt gttgaagcaa cggcatcgcc acatacccgc
    24841 ctccgattga aaagagaccg atttggaaga agcttaagaa cagctggaga tagagcatca
    24901 tatgtcttcc tccatccgct tcttcctcct cctcatgtca aggaggttgg ccgtggtgga
    24961 gagcgccagc atcaccagaa tcagtgcgat catgctgacc ttcagaatat agagcaggac
    25021 gaaactgatg atgaagagca gaaccgccgg aatcgctttc actttgagaa acggtttgaa
    25081 gaacttccac gaaacctgaa ggacgatcgc tgcgaccacc ggtctcatcc cgagaaacgc
    25141 cgcgtcgacc cagacgttgc cctggagtat ggtgtagatg acgctgatga tcgagacgat
    25201 gatcatcggc ggcagaaccg tccccaacgt cgcgacaagc gcacccggag ttctcgccag
    25261 atgataaccg accatcatcg ccacgttcac ggaggcggat cccggtgcgg attgggcgat
    25321 gatgacgaag tccatcatct catcctggtc gatccagccg agctttgcga ccatcttctt
    25381 gcgcatcagg ctgacgatga caaatcctcc gccgaagccg gacatgctca aggtgaatgt
    25441 ctcccagaac aggcggagaa agaattttac atttacttcc ggtttcccct tcttgatttc
    25501 agttttcatg cagtacccca ctaaggagga gttatctgct tggccacgta gtgcaatcgt
    25561 tcaatcacat aggggaaaaa ccccatcatt gtcgaacaga agcgataccc gtcgtcattt
    25621 cgataacgct tgcaaccgcc tctacacaag tgcttccacg gacatgtctc acacccatcg
    25681 atcgtgttag aactctcctc gatgaagcgc attccccggc gtcgttcatc gagggtcgaa
    25741 aattcatcga aaaggatggt tcccaatcga tgctcatcag tgcaatagaa atcgcaagga
    25801 tacacatccc cgttggcttc gaaaacgtat tgcacggaac agatgccggc catatcgcaa
    25861 ctctcagggg gatagccggc tatcatcccc acgagattat cgaaaaaacg gtttgagacc
    25921 ggggtttggt tcatgaatga atcaaaccac aagtcgaaca tggccttgag gaatcgttca
    25981 tacccctcgg cactcaaaaa acgcttcccc ccctgcatcg gatcgatgca ggggatgtac
    26041 tgttgatagt gtagcccatg ctcgttcagg aaggaataga gcatttgcac attatcaacg
    26101 agatcgttcg tcacaacgct caggatgttg aagtccaccc cttcctttgt gaacagatca
    26161 atcgtattca acacgctccg gccacttccc agaccttggc ggtcgacgcg atggaggtca
    26221 tgaagccggc gcgtcccatc aaacgagatc cccaccagaa acccatgggc tttgaagaag
    26281 tcggcccaag accggtccaa ccccaccccg ttggtctgaa acgcccaggt gatccgttgc
    26341 ccctccccct gccgttcatt gacgcgcgaa acaaagcgct cgaagaatga aagaccggcc
    26401 aaggaaggct cccctccctg gaagacgaac tggatgttcc gagcaagagc catgctcttg
    26461 tcgatgatct tgcccgcaac ctcatcgctc atgaccgggt gatggtggac gctcctgttt
    26521 ctcgccgtat cgaggtaaaa acagtagtca caggcaagat tgcacgcagc cgacgccggt
    26581 ttgatcatca tcgtcagatg ttcgcgcatc tccccacccc ccccctagta aacctttatg
    26641 atgaaccgtt ttaaggaaga atgcaagagg ttttttgaaa aagcatgctc atattcaaag
    26701 aaaacacccc gaggaagatt acaatgatcc caggtgcctc ttcagcgcca cgaccgatcg
    26761 gctgttctga ttgatgtaat gtatgctctg cttacgtgtt gcctgttata gtatcgttcc
    26821 ttccgaaaga atatccacat attttttata gacatattgt ctgtatctct tcttttcctc
    26881 atctacaagt tccaatatgc caagttccgt taagttgtcc aaaatcttat gtatggtcgg
    26941 cttgctgagg tctaataaat cttttatatc atctgcttcg ataaacggat gtttcagtaa
    27001 ataatcatac actgaaagca aatttgataa attccctttg gaacatgaac gaattttttc
    27061 cgtgtcattc tttttgagtt caacaatttt ttgaattgac atgatagcat cttcagatat
    27121 ttcacaaata ccttctatga aaaatgtaat ccattcttca aaatgtccct tgatacgaac
    27181 attattgaga agctcataat atgtattcct gttttttttg aaaaaataac ttaaatacaa
    27241 aacaggatat tcaattaagc ctcgttcttt taaaaataaa acaatgagca atctgcccat
    27301 tcttccgttt ccgtctaaaa acggatgtat cgtttcaaac tgatagtgaa tcaaggcaat
    27361 ctttatcaga tttgaaattg cagtttcctc atgcatatat ttttctaaat cactgaggca
    27421 aatatccatc tcactcggtt caggaggaat aaaacttgcg ttctgtagcg ttgaacctac
    27481 atgacctatc caattttgag attttctaaa ttcacccggc attttttctt cccctctaac
    27541 attagatagc agcactgcat gggtttcttt aatcaatctc atacataaag gaagctcatt
    27601 gagccttttg aaagcgtagt tggttgcttt gacgtagttg acaatttctt ccgtatcttt
    27661 tgttttttta tttttcctat ccttttgcaa tacatccacg agcgaagcct gcgttccctc
    27721 tatttgagaa ctgattacag cttctttctg gacatacatg gatacgaaaa ggtcaatatc
    27781 cggaagcgta atcgccatac catctaatct tcctaaaagc agatatgctc tatgtatcaa
    27841 cctattcata tcatcatcta tagttattgt caggtctttt agattgtgag gaataaaaca
    27901 cccgtagcca ctctgcattc tcttcactat tcctgctctg ttattttcca atttcaagat
    27961 catcacctct taagttaaat gtattttact taacgtaaac ataccttttc taagtaaaat
    28021 tttcaatcat ttattttact tataaataag gctagacctc taagtaacat ttcttttcat
    28081 atagaggctc ttgcaaaata gaaaaagccc tgtatttttt cctgattcaa atgctcgcac
    28141 gttgaagagt cttcgggcaa acgggatagc atacaacccg tttcggttga gagcaatgga
    28201 tctgacaatc ctctatcatg tgcatatcac caaaccctcg gtgttgccgg gctggtattg
    28261 gggttatctt tcgaagaatg caggagggtt tttgaaaaag catgctcgta gccgacaagg
    28321 aagatcataa tggccccaga tgcctcttca atgcttcgac cagccgggtg ttctcttcct
    28381 tcgtccggac cgcaagccgg atgcactgtc ttggagcaat tcccttcttt ttggagaggt
    28441 ccttgatcaa cagcccctcc tctacaagga gcctgaccgc caggtcatgg gaggagacgg
    28501 gcgcatgcac ttcgcagagg atgtagttcg cccgggaagg gaacacggtg aggttttcaa
    28561 ctgattccag ctccttctca aattggatcc gggcttcctt aatcagagtc agggagcctt
    28621 gatagcttcg gtacttgccc cagatctgca tgaagtattc tgcaaatgag ttgatgttcc
    28681 agatcgacat ctccttggag attcttttca tgatggtccc gttcgtcgtc gcaagcaccc
    28741 cgaggcgaag accgggaacc ccgaaggatt ttgagatcga tttgatgaca acgaggtggg
    28801 ggtttttgtc aagaagctcc tggtcgaaca gcgtctccgg cattgttttc tcagtggtac
    28861 tgcccaccta tcgagtggcc caattcgttg aacgggcgat cgtctccctt caaagccagt
    28921 gcttcaaagc ctttgaaatg atctttgtcg acgattgcgg caccgatgga agcatcgaca
    28981 ttgtcgcccg gcatgccgaa agggatccgc ggatccgcat cgtgaagcat gaacgaaacc
    29041 ggggaaccta tcatgcccgc agaatgggcg tcgaacacgc aagaggaaga tatatcgtct
    29101 ttctcgaccc ggatgacgag ctggatgagc attgtctgag ccaactccat gaacatcttc
    29161 atggttcgcc tgcatcggta atcttctatg gtgtgaaaac cgtcccccct cccccatggt
    29221 ataaatcatc gacacggtcc tttcccgtcc ggtcaagaga gcgcttgctc gaaagcatgt
    29281 tcaaaaaatc tatcggcgga tacgccttca cgacgctggg aacccccggg aaagcctacg
    29341 atcgcaagct cctccttgcc gtctatgatg agctggcgat acccggcgat ttcagatatt
    29401 tcttctccga agacagcgtg gtgctctttg catgcgcgat gaaggacccc gttttccttt
    29461 cagtcgaata tgacggatac atctaccatc tgaacccttc ggcgatcacc agcaagaagc
    29521 ggatgagctt gcgttcccaa catatcttcg atcaggtcgc cttcacgatg gacaagctcc
    29581 aacacctgat caatcgacat caccttgcaa aaagcgagcg cacgttcctt caccactaca
    29641 tccatcacaa taccaaaagc gagctccttt tgctgaagcg gttcgattca agcggcgccc
    29701 gctatttcac atccgtcgct tccgcccttt tcgcccggcc aaggctcaag cacgcggcga
    29761 tgctgctctt ctttgtggtg acgctcggta gaaaaagact gtagaagccc ggatacgcat
    29821 tatttttaca actcccgttt tctcttcttt tctttgacaa tgaagcaaat catttatagg
    29881 agcatgccat gagcagcaaa caagaagaaa cgaagaaggt cgggaaagcc caagtcatca
    29941 ttctttccac cttcgcggtc atcatcgtcg ccctttcggt gttcgggtgc tacgggtgca
    30001 gctatcatcc ggtcagtccc ccgacctcgg aggaggcgct ctcgatcgtc gagcggatca
    30061 gggaaaccgt gtgggagctt gatgaggccg gtgaagtgaa gacgatcggt gaactgggcg
    30121 gaaagctgaa aacgattgag atcctctcaa gcggagtcga gggtgtatca cttgacgtat
    30181 tgctggagtt tgaaaactcg atgccgaagg agggcaagct cctctttctt gaggacagcg
    30241 gcttttcatt aagcgaaagc ggcatccctc tgaatgtaac gatcgtcttc tccaacagca
    30301 aagacggaaa cagcgagacg ctcaccctca agggaaatga gtcgaattcc tatctttact
    30361 acttgaaaat ctaacggttt gttacgatag ttgaatcaga ggttcgatcc gatgcattcg
    30421 gcaaaggaaa gaacgcttga tgaggaatac gcctatggta cacttcaaag ctcaaaaacc
    30481 tacgtacatg aacggggagt ataccattgt taccaccccg tttctgcagg atatgc
//
LOCUS       DAOSLI010000020        29948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1751_length_29948_cov_27.409260, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000020 DAOSLI010000000
VERSION     DAOSLI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 29948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29948
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_29948_cov_27.409260"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="PLF67_04450"
     CDS             <1..936
                     /locus_tag="PLF67_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE89299.1"
                     /translation="ETLGIVGESGCGKSTAVRSISQLHKPTGGSVIFNGIELTTADSK
                     TMLAARRDIQMIFQDPYASLNPRMTVRSIIAEPLVIYNRRKLLDPPLSKLEIEHRVEE
                     LMERVGLSKVFKNRYPHEFSGGQRQRIGIARALALKPKIILADEPVSALDVSIQSQIL
                     NLLSDLQKEFNLTYIFIAHDLAVIQHISTRVAVMYLGKIVEISEAVRLYDNPLHPYTT
                     ALLSAAPIPDPVVERNRQRIILTGDVPSPDRERFGCYFYDRCPKRMPWCSNHIPPMFK
                     QGENHEVACWLYDKTRDYQDVSMEEAEADAAKTNP"
     gene            complement(941..1495)
                     /locus_tag="PLF67_04455"
     CDS             complement(941..1495)
                     /locus_tag="PLF67_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89300.1"
                     /translation="MLHYAVYQKNSAIVYTLKKRVRMVFLLITLFLLWGLVLNIGEGV
                     SIRSMIHIVLLLIVTTIGVLYRDGWTFDVGNRTIGSWWGIGPWVRRESFRFDEVVRLD
                     LSHFIKGSALDGKILPKRRQKAVVVFSIILTDERQKTVQVIPERSSSGRIEAAFDRIA
                     IFTELPAKIDRPRDVDLPLRMKDL"
     assembly_gap    1707..1806
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1807..3282
                     /locus_tag="PLF67_04460"
     CDS             <1807..3282
                     /locus_tag="PLF67_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE89301.1"
                     /translation="EPESLDPALVQGVPEHRIYMAIFEGLVAMDPKTGNAIPGVAESW
                     TSNEDGTQWTFKLRKNAVWSDGKAITAHDVVWSWLRILDPATGGPYAWFPAMFLVGAE
                     EYNAGLAPASAVGIRALDDYTFQMDLLGPLPYAIDALTHYSFAIMPKHAIEKHGAAWT
                     EPANFVGNGPFILADRVPQTSITVTKSPTYWDKANVRLDKVIFYSSDSDTTNYNMYLN
                     GETDWATNVPTDQIAAAEMRDDFHKSPQLATYYYVFQTEKAPLDNPLVRKALAYSVDR
                     EALIEGITKAGQIPAWGIVPDMAGYKALEFPFDSMDEAVEQAQKWLAQAGYPNGAGFP
                     TVSILYNTSEGHKQIGEFIQQEWKKNLGINVVLENQEWGTYLNSRNVGDFQISRAGWV
                     GDYQDPNTFLDMFITGTAMNGGQYSNEVYDILINEAARIDNPADRFGVLRTAEDIMIN
                     EDQALMNLYYYVTLNMIDTNKWGGWYGNTMDYHPVKDIYLK"
     gene            3372..4397
                     /locus_tag="PLF67_04465"
     CDS             3372..4397
                     /locus_tag="PLF67_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOE89302.1"
                     /translation="MNYYDLRSDTVTKPTPEMRKAIAEAEVGDDVYREDPTTTKLEAL
                     AAELTGKEAALLLTSGSMGNLVALYINGGRGNEALLSSNSHIIHHEIGSVSAIAGVLP
                     IAIEAPRGILSADLLQGKVKRGMYDMATTTLVSVENTIGGYCYPIENLAGIQRFAKKN
                     NLAVHMDGARIFNAHIATGISVKEYAAHADTITFCLSKGLGAPIGSIICGTKEFIDRA
                     LTVRKMLGGGMRQTGIIAAGGLYALNHHVKRLKDDHLHARAIAEALVDAGWADVDLEG
                     VQTNIIFFTVPGIPGSAVLSQLKKIGILANTEGDTVRLVTNLGIDDEDTVEICARLKS
                     FEPEAAE"
     gene            4394..>4786
                     /locus_tag="PLF67_04470"
     CDS             4394..>4786
                     /locus_tag="PLF67_04470"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOE89303.1"
                     /translation="MNHTILCFSGTGNSYHVANRLRIAFENARIVMIPDLMDDSTVEL
                     TEAVGLVFPTYFFLPPATVVEFIETVLPKLDLSPVQYFYVVTTKNLSGGWAHSIAEIL
                     LRDRGVALSYASSVRFPNTYIPLFTAPNI"
     assembly_gap    4787..4886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5010..6275
                     /locus_tag="PLF67_04475"
     CDS             5010..6275
                     /locus_tag="PLF67_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE89304.1"
                     /translation="MNFDTMIDRRSTLSVKWDPSAITNICSNPDAEPFWVADMDFTVP
                     PEAHAEAKALAEHGIFGYPVATDQKTVFMSFVKARHGMTIDEDELSISQGILSSISLS
                     VELFSKEGDGVVVPLPAYAPFMRIVKLHNRTLIPWPMIYDPKNHAFTLDWASLDTVID
                     RAKLLIFCSPHNPTGIVFSEEELIRVAEICKARDVVIISDEIHADLSFKSFVSIHTVA
                     KELEAKAITCMAPSKTFNIAGEHYSVTLFTDSFMKQAFEKRLHQLWMFRPASYVTALA
                     RVSYEKGLPWLEELVTYLKENAVFISDYFARHLPELIFLEPEASFIGLIDAGRIMKLI
                     EADAKANPDLYDPATSPDGSMLSRFLGQRARVACNAGTWFGGQEYASFIRFNFATQRA
                     RIEAALDRIKTAIRFLKETYPPPGGQDAR"
     gene            complement(6211..7179)
                     /locus_tag="PLF67_04480"
     CDS             complement(6211..7179)
                     /locus_tag="PLF67_04480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE89305.1"
                     /translation="MKEMVHNALYVEERDQLLFARRFSPFVAKELGRHALYQAMGKTS
                     ASVQLRFLRTGPATLTLKRFPASLRSRPGQIDFSRRYGGPLNLSETLDIEVDGMLFHN
                     PLRNGVIRFNEGEEITIHLPNHHEVGWILEGSVEPVERNTGTLLCLGDSIIQGVGVHH
                     GSEGLCTRLGSILEMEVLNQGLAGTLVNPRMVVPLEKAIDCVLLGYGTNDWSLREDRS
                     EFIEDVDELFSRLRTLYPHQPIVLLTPLYRADHDRIRPLGSFEEIGTILGEAASRYEN
                     VTVLDGVSLSLRDQFDDGFLHPNSRFIEHLARQVADRFPSGSGSLS"
     gene            complement(7176..8306)
                     /locus_tag="PLF67_04485"
     CDS             complement(7176..8306)
                     /locus_tag="PLF67_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE89306.1"
                     /translation="MKHIGLDLRFQDGYDMEVAGGVVRYARSKSDWILRGQGPWFSFK
                     EDLSECDGLIARIESKEVADEVRGYRIPVVDIASAVRLPTLAQVHNDDHLTGKRAGSY
                     LKGLGIEHFGYCSAEGLIWAKERLRGYQEEVQREIFHFSRPLGWWRALYGDDEELGRW
                     LTSLPKPIALFCCNDLSALKVQQQLKHLSIAIPDECVILGVDNEQLVCELANPSISSI
                     QIQCSEIGYQASKLLDELLAGTAEGAVRRVIEPGALVERESTRLVSENDEHVSKALLL
                     IKARATGGLTASDVASSATICRRALEIRFQKILGSTIWKEITKAKLNHATTLLKHTTD
                     SIEQISEACGFGSIHRFYTLFKRTYRTTPQGYRNREQGRKQG"
     gene            8395..9360
                     /locus_tag="PLF67_04490"
     CDS             8395..9360
                     /locus_tag="PLF67_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE89307.1"
                     /translation="MKMLHIPKIGMGTFGSDHISANVMASSVKEALAIGYRMIDCARV
                     YNNEREIGEVLAASKIKRSDLWLTGKVWNDMHGKANVIKSAELSMKDLQTDYLDLFLV
                     HWPFPNHHPPFAHVDERNPNAVPYIHKQFMDTWSGMEELQRKGMVRHIGVSNMTEAKL
                     RLLLGDCSIRPFAAELELHPTFAQKELRSYMKAEGIIPIGYSPLGSPNRPERDRTDED
                     VVDMEHPIVVELARKHDVHPASICLKWASGNGIIPIPLSTKVKNLRSNFESVHSDPLT
                     EEELTMLEAVDSNNRLIKGQVFLWEGATSWRDLWDEDGTITGGQS"
     gene            9357..10409
                     /locus_tag="PLF67_04495"
     CDS             9357..10409
                     /locus_tag="PLF67_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOE89308.1"
                     /translation="MMKAAFLPGNSTVVFKEVPIPKPGYGQVLVKTKASTICGSDIRA
                     IYREHLGKGPEGYQNVIAGHEPCGQIVEVGEGMRRFKKGDRVIVYHISGCGICYDCRQ
                     GFMISCTSHLRAAYGWQRDGGMAPYILCDEKDLVLLPDELTYADGAQVACGFGTVYEA
                     IEKVGVSGNDTVLVVGLGPVGMATLMLAKAMGATKLFGIETNVDRIEMCKELGILDYG
                     FTPSPTVVAEIKALTGGHGVERAFDCSANNEGRATAIRATRKWGKIAFVGEGGSVTFN
                     PSEDMLHDQKTIYGSWVTSIWKMEDLVERLVRWNIHPEDLITHRFSLDDADKAYALMA
                     SGKCGKVCVCFDEELK"
     gene            10469..12079
                     /locus_tag="PLF67_04500"
     CDS             10469..12079
                     /locus_tag="PLF67_04500"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOE89309.1"
                     /translation="MSLVLGVDFGTASVRSIVVDPHTGSILGSGEYAYQHTPLYSNPF
                     MVRHHLEDYRLGLEQSVKDAMKEVDASLIAALGLCSTGSSILPVREDGTALLDLPAHR
                     SNPNAHIWLWKDHTPIEEAGTINSLIHRSHPNYLSRIGSVYSAEWYWSKVLHCLRVDP
                     ELFDEAYTWIEITDYLLFLLSGKTHIDEAIRNISAASHKALYDGGYPPLSFLGELDER
                     LIPLGRSLHRSTVQPCDRRSGNLSGEWAKRLGLQEGITLATGALDAHFGALGAGVGAG
                     TLVKIVGTSSCDLTVAPLTSHFSDTKGLGGVVEHSVLPGMWGIEAGQSGVGDIFDWYV
                     NTFLGTTHQALMDEAATLRPGQSGLLSLDWHHGNRSILQDQNLSGLILGLDLSTTPAE
                     VYRALIEATAFGSKVILDRLQACSVQLEKVVLAGGIPMKNPLLVQIYADVHERPMHLA
                     ECEYATALGSAIASSVASGIHPSIEAAQKAIVPPSTRVVHPNEKATALYRRLYPIYRT
                     LHDGFGVADAAVDLYPVMKELLALKSGG"
     gene            complement(12063..13493)
                     /locus_tag="PLF67_04505"
     CDS             complement(12063..13493)
                     /locus_tag="PLF67_04505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOE89310.1"
                     /translation="MKKRIAIDLGASNGRVLVGNLEEFEVVHRFTTHNDQILGEFYWN
                     IQGLFSEIKTGLKKAFDCYPDEIVSIGIDTWGVDYALVDGNGSLVSLCYHYRDGRTDG
                     LIEEVSALLGGKERVFEETGIAFQPFNTIYQLVAHKRDRGEVFAAAKHYLALPDLLAY
                     WLTGVMKNERSHASTTQLYNPKTRDWAWPIIDELGFDRSLFGKIVDSGTVLGPLTVDL
                     QHEVGASDKVVVIASAAHDTASAVAAVPAIAGQVPLYISSGTWSLLGSELGEPLTTVE
                     AMESGFTNEVAANSQIRYLKNIMGMWIQQECVRHWESQEGKLTWKELDEQTLLEEAYQ
                     GSIDVNDLRFLKPNTYDNLMVDRIDAYLEEHGMEKPKNKGQYMVAIYRGLATAYAEAI
                     GDLERVLGVSFASLNIIGGGSKNEILNQWAADATGLTVLAGPVEATALGNLIVQSWAT
                     GELASLQEGRDLIRTLHKVKTFTPRS"
     gene            complement(13496..14770)
                     /locus_tag="PLF67_04510"
     CDS             complement(13496..14770)
                     /locus_tag="PLF67_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOE89311.1"
                     /translation="MKTRAVRLYGINDMRIEEFELPAIKDDEILAKVITNSICMSDHK
                     AAAQGPNHKRIPKDIDKNPVMLGHELCGEIIEVGKKWKDKYHEGMRFTIQPAMNYKGT
                     LDAPGYSFPYIGGDATYVILPHMLMELDCVLPYAGPSYFLGSLAEPLSCVVGTFHAMY
                     HTTAGSYVHHMGIVEGGNAAILAGVGPMGMGAIDYAINNPDRRPGRLVVTDINQERLD
                     RAAMLYTVEHAKKNGVELHYLNTRDLEDADSVLMELSNGEGYDDVFVMAPVRALIEQA
                     DAILAKDGCLNFFAGPERTDFTASLNFYNVHYASTHIVGTSGGNTDDLRESLKLMEQG
                     LINPAGMVTHIGGLSSVPQTVIDLPKIPGGKKMIYTHLDFPLTALEDFAEKGKKYPLF
                     AKLDELVKKHNGLWNAEAEAYLMEHCTMRIED"
     gene            complement(14781..15932)
                     /locus_tag="PLF67_04515"
     CDS             complement(14781..15932)
                     /locus_tag="PLF67_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE89312.1"
                     /translation="MTVFEDIKREIPPLIRGLSFDGSLGLIIHTLGPGPSALVLPSPS
                     KGDSMKETAKAWQALFDEYVKKERVYPATVGVGELRYGLGTNYDEAVRGEGVSKLPTL
                     PPALTRSDVVRDKVAVVTGGAQGFGEGMVRSLVDHGAFVYVADLNREGAQALASELNY
                     DAMITVAKAVEVNVTDEASVQAMMDTVVSEVGGVDLFISNAGVLRAGSVKTMSLKDFA
                     FVTDVDYTGFFICTKFASRTMAIQNIPSGAYYTDIVTISSKSGLEGSNKNGAYAGAKF
                     GTIGLTQSFALELVEDNIKVNAVCPGNFLDGPLWSDPKRGLFVQYLETNKVPGAKTIE
                     DVRRAYEAKVPMNRGCTTADVMKAIFYIVEQTYETGQAVPVTGGQVMLN"
     gene            complement(15929..17251)
                     /locus_tag="PLF67_04520"
     CDS             complement(15929..17251)
                     /locus_tag="PLF67_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOE89313.1"
                     /translation="MTVSDAKIKINDPDKSVRLAAAAVLGKAIREGVLANTPSLEVNN
                     HVHTTYSFSPYEPAAAAFAAWQAGLGIVGSVDHDSISAAEEMNEAASLIGIASTVGFE
                     LRVSFLDTPLAKKKINNPDSEGIVYICIHGVPSGRIEEVKAFLAPLQAIRNERNRKQV
                     GKLNTLLKRYGIVVDFEGDVVPLSRAGEGGSITERHILHALSKKLIASYGKGETLLRF
                     LRCELKLDIPPKVEKFLLDGANPHYEYDLLGVLKSAFLPSFFIQPSREESIDVREAVA
                     FAKSVGAIPAYAYLGDVASSPTGDKKAEQFEDGFLDELLDLLVEIGFPAITYMPPRNT
                     KEQLARIQRLAQERNLMEISGVDINSSRQSFSCPELLEENARHLIESAWALVAHEKLS
                     SVDPVYGLFHPENPYRDEALNQRIARYAAIARNSDVHNPYTMIEEVRS"
     gene            complement(17253..19706)
                     /locus_tag="PLF67_04525"
     CDS             complement(17253..19706)
                     /locus_tag="PLF67_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOE89314.1"
                     /translation="MSRQIPFDPATLRKKDTLVLPEIPINHYVSDVKKELKTYGKERL
                     IRAYYDMLLIRKFETMLDMIKKEGVYQGISYKHLGPAHLSAGQESAAVGQAMVLDVDD
                     QIFGSHRSHGEILAKSMSAIAQLSEKELLSIMESYMGGNTFKVVEKHHKGKTVKDLAE
                     NFVLYGALAEIYAKKNGFNAGLGGSMHTFFKPFGSMPNNAIVGGSGTIAVGAALYKKI
                     NRKKGIVIANIGDGSLARGPVYEALVLASMDQYTTLWDDNPGYPPFLVNVFNNLYAMG
                     GQPSGETMGYGIAARMGAGINQNSMHTERVDGFNPLAVADATARKKALLLEGKGPAML
                     DTITYRYSGHSPSDAMTYRTKEELDAFRNQDPIEAYGEYLVKNKIASASELESYDALL
                     EEKMRKTLEITVDPAISTMVDEAFIESVMFSNGSVEKFDTTEPALLQTLEENPRVQQI
                     AKRSRYAYDEEGKMYPAARLYQYRDAVFEAMVHRFAIDPTMVAYGEDHRDWGGAFAAY
                     RGLTELLPPSRFFNSPISEAAIVGSGVGYALAGGRAVVELMYCDFLGCAGDEVFNQMP
                     KWQAMSGGQLTMPLVLRVSVGNKYGAQHSQEWTAMVGSVPGLKAMYPATPYDVKGMLN
                     YALRGTDPVVFFESQKLYGIGEQFVKEGVPEGYYEIPEGEPAIRKEGKDITLIALGPS
                     LYTAMKASEKLAELGVDAEVIDLRWINPLKYELLVESVKKTGKVVLVTDSAERGSYLH
                     TVASNLNRLAFEYLDGPAVVVGSKNWITPPAEMEEYYFAQASSILDAIHEQIMVLPNH
                     VVKQNYTDSHFYRTSSEGV"
     gene            complement(19727..21076)
                     /gene="lpdA"
                     /locus_tag="PLF67_04530"
     CDS             complement(19727..21076)
                     /gene="lpdA"
                     /locus_tag="PLF67_04530"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOE89315.1"
                     /translation="MAQYDLIVIGSGPGGYIAAQRAGTLGKKVLLIEKEHYGGVCTNK
                     GCIPTKSLLGSAKTYEHAKNGSLFGVDAKDIAFNLKSAMAHKQDTVETLRGGIEFLMK
                     SAKVETVFGEAKALDAHRVEVNGTIHECSYLIIATGSSAFVPPIPGADLPHVLTSDDI
                     LEIESLPSELVVIGGGVIGVEFASFFSMVGVKVTVIEMLDEILPMADAEFGKLARRAM
                     KDVTFNLGCKVEKITEKSVVYTDAKGERKELAAEKILMSVGRRPNIGGLEALGLKIEK
                     GAVVVDEQMRTSLPTVYAIGDVNGKSLLAHSASRMADVAVSTIFGKGMKMRYDAIPWV
                     VYSNPEIAGCGLTEAQAKERGYEVLVHTAQGRQSGRFLAEQGKRAPGMIKVVADKESG
                     VILGVHLLGPYSSEIIWGVAAILEAELRIQDVKEIVFPHPSVSELLKDAVFALDSSL"
     gene            complement(21063..22388)
                     /locus_tag="PLF67_04535"
     CDS             complement(21063..22388)
                     /locus_tag="PLF67_04535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOE89316.1"
                     /translation="MAEQVLMPKQGNTVESCIIVEWNVNVGDKVAVGDILLSAETDKS
                     TIDVESTAAGVVLARLWEEGDDVPVMEPILIVGEEGESFEIQRPKKKEEEKSVQVEEP
                     ETVKKEPKTASAPVSAPAAGGSTPRARKLSEQLGIPLSAATPSGPKGRIVEKDIRALA
                     GSPLSPAAKAQALKEGVVPPSVGSGMGGRVLARDVSKTPERVVVDLDAHTDYPVRGIR
                     KVIARRMVESLQTTSQLSLHGWADARALKALRAAFKEDEETSGITIGDLVNFAVVRTL
                     LEFPAFNAHYLGETIRFFEHVHLGVATDTEKGLMVPVLFGSDLMSLKELSARTKVLAT
                     RARDGVAQPDELAGSTFTVSNVGSFGIEAFTPVLNIPEVAILGVGTIVLKPVSTKNGV
                     EFIDHIGLSLTMDHQAVDGADAARFLRALIDRIASLDRLLIRMGSNGTV"
     gene            complement(22399..23154)
                     /locus_tag="PLF67_04540"
     CDS             complement(22399..23154)
                     /locus_tag="PLF67_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOE89317.1"
                     /translation="MFGLSPREEEILTLLRSGKEYPVTRLAEELGVSAVTIRSDLRSL
                     DTKGLIVRSHGKAVVATTPQVGMRNDAHTKIKEAIAKAAAKLVKDGDSIMITNGSTCS
                     LIPRYLFGKQSIKVVTNSTLLLPYGRANNHLNITLIGGEYRPEAEALVGPAAITHIES
                     YHVQTTFFGTDGLTLAHGLTTSLVENAQVVQRMCAQATRRVLCVDSSKVGNRGFVRIM
                     PVSDVDTIVTDSGFPRDLVSELETLGVEVLIVQ"
     gene            complement(23275..23583)
                     /gene="rhaM"
                     /locus_tag="PLF67_04545"
     CDS             complement(23275..23583)
                     /gene="rhaM"
                     /locus_tag="PLF67_04545"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOE89318.1"
                     /translation="MREAFVMYLKKGHEEEYERRHSEIWPELAALLKESGVSEYSIFL
                     EKETGKLFAVQHNTGQGSQDLGQNPIVQKWWAYMADIMETNEDNSPISIPLREVFYLP
                     "
     gene            complement(23593..24633)
                     /locus_tag="PLF67_04550"
     CDS             complement(23593..24633)
                     /locus_tag="PLF67_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89319.1"
                     /translation="MREKRELMAKTRLIDESSLKERLITIFTMWEVLLSIIFILIIIF
                     FSTRTRYFLNWFNLMNATFQFSEKAIMALPMIFIIMCGDIDISIASIIALCAYAVGNA
                     AAGGASIPTLILIALAVGTLAGFFNGFMVTFLEMPAIAVTLATQSIYRGIAIGMLGDQ
                     ARTTFPKTYGFFGQEFIKGTTIPFEFVLYLVLMVIFGFILHKTTYGRRLYAIGNSAEA
                     ARFSGINVKRMRIINFTITGFVSGLTAILLTSRVLSVRSNIATGWDMEIITLVVLGGV
                     SITGGKGTVWGVFLGSLLVGYLKFGMGLMKISGTLMTIVIGSLLIIAVLLPRLLDLYK
                     ANRKLRKQHEMR"
     gene            complement(24633..25625)
                     /locus_tag="PLF67_04555"
     CDS             complement(24633..25625)
                     /locus_tag="PLF67_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89320.1"
                     /translation="MPSNTIKKILARREAALVILLAVLLIPITIRSPQFISVRNISTI
                     LNDMAILAIVAIGEFFVILSNGIDLSVGSVVAFTGMACGLINEANQAFGAVPLLFVGM
                     GIGLVMGLVNGVLVAYGKIPPIITTLGTVSIYRGLTFLLSGNQWVTAHELAPSYIAFP
                     RVTFLGISLLLWIAFIVIAVFYYFSRFTRTGREVYAIGGNPTAAKFVGVNESKIRVVV
                     FAISGTLSGLAGALWTARYASAVNEMATGFEMQAVAACVLGGVNFAGGAGAITGVVLG
                     TLFLGVVTNALPVVYLSVFWQTFVQGLIILTALTLNTLSDRRKHTKLLAQRRAS"
     assembly_gap    25637..25736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25770..27284)
                     /locus_tag="PLF67_04560"
     CDS             complement(25770..27284)
                     /locus_tag="PLF67_04560"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE89321.1"
                     /translation="MNQPLVKVQNLTKVFPGIRALDDVRIEIRTGEVHALIGENGAGK
                     STLVKILTGVYQPTGGSVFLEGEEITFRNAIDAQEAGIVAIHQEASMFPELSVTENIY
                     MGHHVRHPKTRKLDWKQMRKETQALLARMQLSINPDTLVKNLSVAQRHMVEIAKALSL
                     DAKLVIMDEPTSALTDREVEDLYRIVRQLKAEGKAILFISHKFSEIFAICDYYTVLRD
                     GQYIGEGRIDRSSEDEIIRMMIGRSLDQLYPDHVPNWGKEILRVDSLSQLGAFRNVSF
                     TLREGEILGLFGLIGAGRSEVVRTIFGVDKASSGTITFEGKPFMPKSPYDAMRRGIAL
                     VPEDRQKQGLILKMSLTHNISLPVLDALSWKRSITRKKEEATYVLDHGQRMEIRATGY
                     HVDAETLSGGNQQKVVLAKWIGTEPKVLILDEPTKGIDVATKAAVHEFICEMAKRGVA
                     IILISSELPEVIGISDRILVMHEGRQTAILDKSEANAESVMRWAISTEEMDNAE"
     gene            complement(27361..28371)
                     /gene="rhaS"
                     /locus_tag="PLF67_04565"
     CDS             complement(27361..28371)
                     /gene="rhaS"
                     /locus_tag="PLF67_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE89322.1"
                     /translation="MKKTLFVILIIGLAFGTLFAAGTKEDAKAQKEIVILVKNMGNGF
                     FDAVFKGSEEAAAELGGIKTVYMGPSQPTAEGQIEIIESLIAQRVDGIAISANDADAL
                     IPVAKKAMAAGIKVISFDSGINVGGRIVDLLPSNAELIGRQQIQLAAELANSAGDVAV
                     LSASAQATNQNLWIDWMKEEIKDPKYSKMKLVEVVYGDDLPDKSYREAVALMKKYPNL
                     KAIICPTTVGLLATAQAVKDEGKAGIVEVTGLGLPSEMKGYILDGTCRQMALWNPIDL
                     GYTSTYLLNALIDGTKKGSVGEEIPAGRMGNMKVLEDGIIFMSTPYVFNKDNVEMFAA
                     YF"
     gene            complement(28426..29490)
                     /locus_tag="PLF67_04570"
     CDS             complement(28426..29490)
                     /locus_tag="PLF67_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOE89323.1"
                     /translation="MSLQALINASRRYGSDPHLVLLGGGNTSYKEGNILYVKASGHPL
                     GSIDEGGFVRMDRSKLEAIWNQKFSNDDAKREAEVLQVMMASRLEGETARPSVEALLH
                     ALLPFTYVVHLHPSLVNGLTCAVEGAKYARELFPEALWIDLVKPGFILADTIKRAMMP
                     LESVPNLIILQNHGIFVGGESLEEIDELYRHVLSTLKSNLKRDVDERPVEIDSTIVDP
                     IKEELKTLFAGEVAYFHSNDTRSLLESAESFYPVSSSFTPDHIVYSGFRPLFVPKGDS
                     ITEAFEAFRKKHGQNPKVVCVQDAGVFADGEKPLMLFIDTVRISVYSESFGGPLFMTD
                     EMIEFIRNWEVEQYRAKVTT"
     gene            complement(29487..>29948)
                     /locus_tag="PLF67_04575"
     CDS             complement(29487..>29948)
                     /locus_tag="PLF67_04575"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOE89324.1"
                     /translation="EDVSDKLSSILLFDDELLMHVSRPMHWDSDHVVLFDDKTRRVAQ
                     ELVRSGKLEGCHLGLDFFDASINRIGAWVTGARSLRKALLFALLEPTDQLIEYEEAGN
                     GYAAMALSEHLSVLPFGDVWAEYLRRNNVPSEGELIQRVKAYEQVILKERL"
BASE COUNT         6133 a         8918 c         8184 g         6413 t
ORIGIN      
        1 gaaactctgg gaatcgtcgg agagtcgggg tgcggaaaat ccactgcggt ccgctcgatc
       61 agtcagctcc ataagccgac cggcggctcg gtgatcttca acggaatcga attgaccacc
      121 gccgattcca aaacgatgct cgcagcccgg cgggatatcc agatgatctt ccaggatccc
      181 tatgcatcgt tgaacccccg gatgacggtg cgttcaatca tcgccgagcc tttggtaatc
      241 tataaccgcc ggaagctgct tgatccgccg ctttccaagc ttgagatcga acaccgggtc
      301 gaagaactga tggaacgggt cggtctttca aaggtgttca agaaccgcta cccccacgag
      361 ttcagcggcg ggcagcgcca gagaatcgga atcgcccgag cccttgcgct caagccgaag
      421 atcattctgg cggatgagcc ggtctccgct ctggacgtct cgatccagtc gcaaatcctc
      481 aatctgctca gcgacctgca gaaagagttc aacctgacct atatcttcat cgcccacgac
      541 ctcgccgtca tccagcatat ctccacccgg gttgccgtga tgtaccttgg gaagatcgtg
      601 gagatcagcg aagcggtgag gctctatgac aaccccctgc acccctacac cacggcgctc
      661 ctgagcgccg ccccgatccc cgatcccgtc gtcgagcgga atcggcagcg gatcatcctg
      721 accggagatg taccctcccc cgacagggag cgcttcggtt gctacttcta cgaccgctgt
      781 ccgaagcgga tgccctggtg ctcaaaccac attccgccga tgttcaaaca gggagaaaac
      841 cacgaagtgg catgctggtt gtatgataag acgagagatt atcaagacgt ctcgatggaa
      901 gaagccgagg cggatgccgc caagacaaac ccgtaatcat ttacaggtct ttcatcctaa
      961 gcggcagatc aacatccctt ggccgatcga ttttggcagg aagctcggta aagatggcaa
     1021 tccggtcaaa cgcggcttcg atccgcccgc tggaggagcg ttcggggatc acctgaacgg
     1081 ttttttggcg ctcatcagtc aggatgatcg aaaacaccac caccgccttc tggcggcgct
     1141 tgggcaggat cttcccgtcc aaagcggatc ctttgatgaa atgggagaga tcgaggcgca
     1201 cgacctcgtc gaagcggaag gactcccgcc tcacccacgg cccgatcccc caccacgaac
     1261 cgatcgttcg attgcccaca tcaaaggtcc agccgtcgcg atagaggact ccgatcgttg
     1321 tgacaatcag aagcaggacg atgtggatca tcgaccgaat ggaaacacct tcaccaatgt
     1381 tgagaaccaa tccccacagt aaaaacaggg tgatcagaag gaacaccatc cgtacacgct
     1441 ttttcaacgt atagacgatc gccgaatttt tttgatagac tgcatagtgt agcatgaggt
     1501 actccgtctc aagtataccg atattaaacc ctttgttaag gcttgtttat gcgctgattt
     1561 gcatatccat acaccaaaag catgccatcc tctatgcttt ttccttgatt catgattaaa
     1621 tgaggattaa gcttcatggt atcgaatagt gaatacccta acgatgataa ggagagatgt
     1681 ttatgaagaa gtttcttgtg attactnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnngagc ccgagtccct cgatccggct cttgttcagg gtgttcccga gcaccggatc
     1861 tacatggcga tctttgaagg cctcgttgcg atggatccca agaccggtaa tgcgatcccc
     1921 ggcgtagcgg aaagctggac aagcaatgaa gacggcaccc agtggacctt caagctgcgc
     1981 aagaacgcag tctggtccga tggcaaggcg atcaccgccc acgatgtcgt ctggtcctgg
     2041 ctgaggattc tcgatcccgc aaccggcgga ccgtacgcct ggttcccggc gatgttcctc
     2101 gttggagccg aagagtacaa cgcagggctt gccccggcga gtgccgtcgg aatccgcgct
     2161 ctggatgact ataccttcca gatggacctc ctcggaccgc ttccgtacgc aatcgacgcg
     2221 ctgacccact acagcttcgc gatcatgccc aagcacgcga tcgagaagca cggcgcagcg
     2281 tggaccgagc cggcgaactt cgttggcaac ggacccttca tccttgccga ccgcgttccc
     2341 cagacctcga ttaccgtcac gaagagcccg acctactggg acaaagccaa tgtgagactg
     2401 gacaaggtca tcttctactc cagcgattcg gacaccacga actacaatat gtacctcaac
     2461 ggtgagaccg actgggcgac caacgtaccg accgaccaga tcgcagcggc cgagatgcgg
     2521 gacgacttcc acaagtctcc gcagcttgcc acctactact acgtcttcca gaccgagaag
     2581 gctcctttgg acaaccccct tgtccggaag gctctcgcct attcggtcga ccgcgaggcg
     2641 ctgatcgaag gcatcaccaa ggccggacag atccccgctt ggggtatcgt cccggatatg
     2701 gccggctaca aagcgcttga gttcccgttc gattcgatgg acgaggcggt tgaacaggcc
     2761 cagaagtggc ttgctcaagc cggttatccc aacggtgccg gtttcccgac cgtttcaatc
     2821 ctctacaaca cgagcgaagg acacaagcag atcggcgagt tcatccagca ggagtggaag
     2881 aagaacctcg gaatcaacgt cgttcttgag aaccaggagt ggggcaccta cctgaacagc
     2941 cgaaacgtcg gagacttcca gatctcccgt gccggatggg ttggcgacta ccaggatccg
     3001 aacaccttcc tggatatgtt catcacggga acagcgatga acggcggtca gtactccaac
     3061 gaggtgtatg acatcctcat caacgaggct gcccgaatcg acaatcccgc cgaccgcttc
     3121 ggagttctca gaaccgccga agacatcatg atcaacgaag accaggctct gatgaacctc
     3181 tactactacg tcaccttgaa catgatcgac acgaacaagt ggggcggctg gtacggcaac
     3241 acgatggact accacccggt aaaggatatc tacctcaagt agattcgacc aaaatgtgat
     3301 catccccccg gcacccgtcg gggggtttct ttaaactgga aattgtatag ccgattcgct
     3361 atagtggact aatgaactat tacgatctga gaagcgacac ggtcaccaag ccgacgccgg
     3421 agatgcgaaa agcgattgct gaagcggagg ttggcgatga tgtatatcgg gaagacccga
     3481 ccaccacgaa acttgaagcg ttggctgccg agctgaccgg caaagaagcg gcattgctcc
     3541 tcacctccgg ctcgatgggc aatctggtcg ccctctacat caacggtgga agaggcaatg
     3601 aagcattgct ctcatccaac agccatatca tccaccacga gatcggctcc gtctcggcga
     3661 tcgccggcgt ccttcccatt gccattgaag ccccgagggg aatcctcagt gccgatctgc
     3721 ttcaagggaa ggtgaaacga gggatgtatg atatggccac cactaccctg gtcagcgtcg
     3781 aaaacacgat cggcgggtac tgctatccga ttgaaaacct tgccggaatt cagcgatttg
     3841 ccaaaaagaa caatcttgcc gtccatatgg acggggcccg aatcttcaac gcccacattg
     3901 cgacggggat ctccgtgaaa gaatacgcag cgcatgcgga tacgatcacg ttctgcctct
     3961 ccaaagggct cggcgctccg atcgggagca tcatctgcgg gacgaaggaa ttcatcgatc
     4021 gggcgcttac ggtcagaaag atgctcggcg gcggcatgag gcagaccggc atcattgcag
     4081 ccggaggctt atacgccctg aaccaccacg tcaagcggct caaagacgat catctccacg
     4141 cccgggcgat cgccgaggcg ctcgttgatg caggctgggc ggatgtcgat cttgaaggag
     4201 tccaaaccaa catcatcttc ttcaccgttc ccggcattcc cggttcggcc gttctttccc
     4261 aactgaagaa gatcggtatt ctggcgaaca ccgaaggcga caccgtccga ctggtcacga
     4321 atctggggat cgacgatgaa gacaccgtcg agatctgcgc tcgattgaaa tcctttgaac
     4381 cggaggctgc ggaatgaacc acacgattct ctgttttagc ggaacgggca acagctacca
     4441 cgtggcgaac aggctcagaa tagcctttga aaacgcccgc attgtcatga ttccggatct
     4501 gatggacgat tcgaccgttg aactcaccga agcggtcgga ttggtgtttc ccacgtattt
     4561 ctttttgcct ccggcaaccg tcgtcgagtt cattgaaacg gttcttccca agctcgacct
     4621 ttcccccgtt cagtacttct acgtcgtcac caccaaaaac ctcagcggtg gatgggccca
     4681 ttcgattgcc gaaatcctcc ttcgagaccg gggagtcgcc ctcagttacg cctcttcggt
     4741 tcgatttcca aacacctaca tccccctctt caccgcaccc aatatcnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnncaac tgcaccggat gcctgggatg ctaccatcgc
     4921 tgcccccgga aagcgatcac cttcaaccgc cgggtttcca ccggccatta cccgaaccat
     4981 gaagcgcact ttgataagga atatcgtgaa tgaactttga tacgatgatc gatcgccgtt
     5041 caacgctatc ggtgaaatgg gacccctcgg cgatcaccaa catctgcagc aatcccgacg
     5101 ccgaaccgtt ttgggttgcc gacatggact ttaccgtccc acctgaggct catgccgaag
     5161 cgaaagccct tgccgagcat ggaatcttcg gctatcccgt tgccaccgat caaaagacgg
     5221 tcttcatgtc cttcgtcaaa gcccggcacg ggatgacgat cgacgaggat gaactatcga
     5281 tctcccaagg aatcctctct tcgatttccc tctcggtgga gcttttttcc aaggaggggg
     5341 acggagtcgt cgtcccgctc cccgcctacg ctccgttcat gcggatcgtc aagctccaca
     5401 accggacgtt gatcccctgg ccgatgatct acgatcccaa gaaccacgcc ttcaccctcg
     5461 attgggcatc cttggacacg gtcatcgacc gggccaaact gctcatcttc tgctccccgc
     5521 acaatccgac cggcatcgtg ttcagcgaag aggagctcat ccgggttgcg gaaatctgca
     5581 aagcccggga tgtcgtcatt atcagtgatg agatccatgc cgacctttcc ttcaaatcat
     5641 ttgtgagcat ccataccgtc gccaaggagc tcgaagcaaa agcaataacg tgcatggctc
     5701 cctcaaagac gttcaacatc gccggcgagc actattccgt taccctcttt accgattctt
     5761 ttatgaaaca agccttcgaa aaacgcctgc accagctctg gatgttccgt ccggcaagct
     5821 atgtcaccgc cctggctcga gtctcctatg agaaggggct gccctggctt gaggagcttg
     5881 tcacctacct gaaagagaac gccgtgttca tcagcgacta cttcgcccgg catcttcccg
     5941 agctgatctt cctcgaaccc gaggcctcat tcatcggcct gatcgatgcc ggcaggatca
     6001 tgaagctcat cgaagcggat gccaaggcca atcccgatct ctacgatccc gccaccagcc
     6061 cggacggcag catgctctcc cgttttctgg ggcaacgggc ccgggtcgcc tgcaacgcgg
     6121 gaacgtggtt cggcggacag gagtacgctt cctttatccg gttcaacttc gcgacccagc
     6181 gggcacggat cgaagccgcc ttggatcgga tcaagacagc gatccgcttc ctgaaggaaa
     6241 cctatccgcc acctggcggg caagatgctc gataaaccgg gagttggggt gcaaaaaccc
     6301 gtcatcaaac tggtcacgca gcgaaagaga caccccgtcc agcacggtga cgttctcata
     6361 ccttgaggca gcctccccga gaatcgttcc aatctcttca aaggaaccga ggggtcggat
     6421 tcgatcatga tctgcccggt agaggggcgt caaaagaacg atcggctgat ggggatagag
     6481 ggttcgaagg cgggaaaaca gctcgtccac atcttcgata aactcacttc tgtcctcccg
     6541 aagcgaccag tcgttggttc catacccgag caggacacaa tcgatcgctt tctccagcgg
     6601 gacgaccatc ctcggattga ccaaggttcc cgccagccct tgattgagca cctccatctc
     6661 cagaatcgaa cccaaccggg tgcacaatcc ctcgctgccg tggtgaaccc ccaccccctg
     6721 aatgatcgag tctccgagac agagcagcgt cccggtgttc cgctcgaccg gttcgaccga
     6781 gccctcgaga atccatccca cctcgtggtg gttcggaaga tggatcgtga tctcttcgcc
     6841 ttcgttgaag cggatgactc cgtttctgag ggggttgtgg aagagcatcc cgtccacttc
     6901 aatgtcgagc gtttcgctca agttgagcgg ccccccatac cgtcgggaaa aatcaatctg
     6961 gccgggcctt gaacgaagcg aagcgggaaa gcgtttgagg gtcagcgtcg ccggtccggt
     7021 tcgaaggaac cgaagctgga cggaggcgct cgtctttccc atcgcctgat agagggcatg
     7081 cctccccagc tcttttgcga cgaagggtga aaacctccgg gcgaacagga gctgatcgcg
     7141 ctcttccacg tacagcgcgt tgtgcaccat ctccttcatc cctgcttcct cccctgttcc
     7201 cggtttcgat atccctgagg ggtcgtccga taggttcgct tgaagagggt gtagaaccga
     7261 tggatggaac cgaacccgca cgcttcgctg atctgttcga tcgaatcggt cgtatgcttc
     7321 agcagggtcg tcgcatggtt cagcttcgct ttggtgatct ctttccagat cgtgcttccg
     7381 aggatcttct gaaaacggat ttcgagcgcc cgccggcaga ttgtcgcgct tgaagccaca
     7441 tcgctggcgg tcaggccgcc ggtcgcccgg gctttgatga ggaggagcgc cttggagacg
     7501 tgttcgtcgt tctcactcac caaccgggtc gattcccgct ccaccaacgc tcccggctcg
     7561 atgacccgcc tgaccgcccc ttctgcggtt cccgccaaca gctcgtcaag caatttggat
     7621 gcctgatagc cgatctcgct gcactgaatt tgaatcgagg agatcgaggg gttcgccaat
     7681 tcacacacca gttgctcatt gtccaccccc agaatgacac attcatcggg gatcgctatc
     7741 gaaagatgtt tcagctgctg ctggactttc agcgcggaaa gatcgttgca gcaaaaaaga
     7801 gcgatcggtt tcggcaggga ggtcagccac ctgccgagct cttcatcatc gccgtagaga
     7861 gcccgccacc accccagcgg gcgggagaag tggaagattt cccgttgaac ctcctcctga
     7921 taccccctga ggcgctcctt tgcccagatc aggccttcgg cgctgcaata gccgaagtgc
     7981 tcgatcccca gccccttcag ataggacccc gctcgttttc cggtaagatg atcgtcattg
     8041 tgcacctgag caagggtcgg caggcggacc gccgaagcga tgtcgacgac cgggatccga
     8101 tagccgcgga cctcgtcggc cacctctttg ctctcgatcc gggcgatcag tccgtcacac
     8161 tcggaaagat cctccttgaa ggagaaccag gggccttgac cccgaaggat ccaatcgctc
     8221 ttgcttcgag cgtatcggac gactccgccg gcgacctcca tgtcgtagcc atcctgaaaa
     8281 cgaagatcca aaccgatgtg cttcatggat ccagaataga caaattcgca aaacgataca
     8341 agatattcgt atatcaagca ttgtatttcc tgcccgcttt gataccgtta agacatgaag
     8401 atgttacaca ttcccaagat cggtatgggt acgttcggtt ccgaccatat ttccgccaac
     8461 gtgatggcct cttcggtgaa ggaagcgctg gcgatcgggt atcggatgat cgactgcgcc
     8521 cgggtgtaca acaatgagcg ggagatcggt gaggtgctcg cggcaagcaa gatcaagcgc
     8581 agcgacctgt ggctcaccgg caaggtctgg aacgacatgc acggaaaggc gaacgtcatc
     8641 aagtcggccg aactgagcat gaaggatctt cagaccgatt acctcgatct cttcctcgtc
     8701 cactggccgt ttcccaacca tcacccgccc tttgcgcatg tggatgagcg caaccccaac
     8761 gcggttccct atattcacaa gcagttcatg gatacgtgga gcggcatgga agagctccag
     8821 cgaaaaggaa tggtaagaca tatcggcgtc agcaacatga ccgaggcgaa gctgcgcctg
     8881 ctccttgggg attgctcgat ccgccccttc gcggcggagc tggagcttca cccgaccttc
     8941 gcccagaagg agctgcgctc atacatgaaa gcggaaggga tcatcccgat cggctacagt
     9001 ccgctcggaa gccccaatcg ccccgagcgg gaccgcacgg atgaggacgt ggtcgacatg
     9061 gagcacccga tcgtcgtcga gcttgcaaga aagcatgacg tccaccccgc gtcgatctgc
     9121 ctgaaatggg catcaggcaa cggcatcatc ccgatccccc tctccacgaa ggtgaaaaat
     9181 ctgcgttcga actttgaaag cgtccactcc gatcccttga cggaggagga attaacgatg
     9241 ttagaggcag tcgattccaa caatcgcttg atcaaaggtc aggtgttcct ctgggaagga
     9301 gccacctcgt ggcgggatct ctgggatgag gatggaacga ttacaggagg ccaatcatga
     9361 tgaaagcggc atttttaccg ggaaactcca ccgtggtgtt caaggaggtt cccattccga
     9421 agcccggcta cggtcaggtg ctcgtcaaga cgaaagcatc gacgatctgc ggcagcgaca
     9481 ttagggcgat ctaccgcgag cacctcggca aaggccccga ggggtatcaa aacgtcatcg
     9541 ccggacacga accgtgcggg cagatcgtcg aagtcggcga agggatgcgg cgcttcaaaa
     9601 agggcgatcg cgtcatcgtc taccatatct cggggtgtgg aatctgctat gactgccggc
     9661 agggattcat gatctcctgc acaagccacc ttcgagccgc ctacggatgg cagcgggacg
     9721 gggggatggc gccttacatc ctctgtgacg agaaggacct cgtgctcctc cccgatgagc
     9781 tgacctatgc cgacggcgcc caggtagcct gcggcttcgg aacggtctat gaggcgatcg
     9841 agaaagtcgg cgtgtcgggc aacgatacgg tccttgtcgt cggcctcggg ccggtgggga
     9901 tggcgacctt gatgctcgcc aaggcgatgg gcgccaccaa gctcttcggc atcgaaacca
     9961 acgtcgatcg gatcgagatg tgcaaggaac tcggcatcct cgactacggc ttcacccctt
    10021 cacccaccgt ggtcgccgag atcaaggcgc tcaccggcgg tcatggcgtc gagcgggcat
    10081 tcgactgctc ggccaacaac gaaggacggg cgaccgcgat ccgggcgacc cgcaaatggg
    10141 gcaagatcgc cttcgtcggc gagggcggaa gcgtcacctt caacccgagc gaagacatgc
    10201 tccacgatca gaaaacgatc tacggcagct gggtgacctc gatctggaag atggaggatc
    10261 tggtcgaacg cctcgtccga tggaacatcc accccgaaga cctcatcacc caccgcttca
    10321 gcctcgacga tgcggataaa gcctatgcgc tgatggccag cgggaagtgc ggaaaagtct
    10381 gcgtctgctt cgatgaagaa ttgaaataag cagctggttt ttacaaaggg ggtggtgatt
    10441 caccctcttt tttgcaggga ggaatgggat gagtctggtt ctcggtgtcg atttcggaac
    10501 ggcgagcgtc cgctcgatcg tggttgatcc ccataccggt tcgatcctcg gtagcggcga
    10561 atatgcctat caacataccc ccctctattc caaccccttc atggtccgcc atcacttgga
    10621 ggattaccgc ctcggtcttg aacaatcggt caaagacgca atgaaagagg ttgatgcctc
    10681 cctgattgcc gccttgggct tgtgttcgac cggctcgtcg attctccccg tccgggaaga
    10741 cggaaccgcc cttctggacc ttcccgccca tcgaagcaat ccaaacgctc atatctggct
    10801 gtggaaagac cataccccga tcgaggaggc gggcacgatc aacagcctca tccaccgttc
    10861 ccatcccaac tacctctccc ggatcggctc ggtgtactcc gccgagtggt actggtccaa
    10921 ggtgcttcac tgcctccgcg tcgaccccga gctcttcgac gaggcgtata cgtggattga
    10981 aatcaccgac tatctcctct ttctcctaag cggaaagacc catatcgatg aagcaatcag
    11041 gaacatctcc gcagcgagcc acaaggccct ctatgatggt ggatatcctc ccctctcctt
    11101 ccttggcgag ttggatgaac ggctcatccc ccttggccgg tccctccacc gctccaccgt
    11161 ccagccgtgc gaccgaagaa gcggaaacct gagcggcgag tgggcgaagc gattgggact
    11221 gcaggagggc atcaccctcg ccaccggagc gctcgatgcc catttcggag cgctcggggc
    11281 gggggttggc gccggaacgc tggtgaagat cgtggggact tcctcctgcg atctgacggt
    11341 tgctcccctg acgagccact tctccgatac caagggtctt ggcggagtgg tcgagcactc
    11401 cgtccttccg gggatgtggg gaatcgaagc gggccaaagc ggggttggag acatcttcga
    11461 ctggtatgtg aacaccttcc tcggaaccac ccatcaagcg ctcatggatg aggcggcaac
    11521 gctgagaccc ggtcaaagcg gtctgttgag ccttgattgg caccatggga accgctcgat
    11581 cctgcaggac cagaacctga gcggcctgat cctcggcctc gacctgtcga ccactcccgc
    11641 cgaagtctac cgcgccctca tcgaagcgac cgcattcggc tccaaggtga tccttgatcg
    11701 gctacaagcg tgcagcgtac agcttgaaaa ggtcgttctc gccggcggca ttccgatgaa
    11761 aaaccccctt ctggttcaga tctatgcgga tgtccatgag cggccgatgc atcttgccga
    11821 atgtgaatac gcaaccgccc tcggcagcgc gatcgcatca agcgtcgctt cgggcattca
    11881 tccctcgatc gaagccgccc aaaaggcgat cgtcccccca tcgacccggg ttgtccatcc
    11941 caatgaaaaa gcgacggcac tctaccgtcg cctctatccg atctatcgaa cgctccacga
    12001 cggcttcggc gttgccgatg ccgccgtcga cctgtatccg gtgatgaagg agctccttgc
    12061 cctcaagagc ggggggtgaa cgtcttcacc ttgtggaggg ttctaatcag gtcgcgccct
    12121 tcctgcaggg aggcaagctc gccggtcgcc caactttgga cgatcaggtt tcccagcgcg
    12181 gtcgcctcga ccgggccggc aagcaccgtc aagccggtgg catcggcggc ccactggttc
    12241 agaatctcgt tcttcgatcc tccgccgatg atgttcagcg aggcaaacga gactccgagc
    12301 acccgctcga gatccccgat cgcctcggcg tacgctgtgg cgagcccccg gtagatggcg
    12361 accatgtact ggcctttgtt cttcggcttc tccatcccgt gctcttcgag gtaggcatcg
    12421 atccggtcaa ccatcagatt gtcgtaggtg ttgggcttga ggaaccgaag gtcgttgaca
    12481 tcgatcgaac cttggtaggc ttcctcaagc agggtctgtt catccagttc cttccaggtc
    12541 agcttcccct cctggctttc ccagtggcgg acgcactcct gttggatcca cattcccatg
    12601 atgttcttga ggtagcggat ctgcgagttg gcggcaacct cgttggtgaa gccgctttcc
    12661 atcgcctcga cggtggtcag cggctcgccg agctcgctgc ccaagagcga ccacgtcccg
    12721 ctggagatgt agaggggcac ctgtcccgcg atcgcgggaa ccgccgctac ggcgctcgcc
    12781 gtgtcgtggg cggcggaggc gatcaccacc accttatccg aagctccgac ctcgtgttgg
    12841 aggtccaccg tcaaggggcc gagcaccgtt ccgctgtcga cgatctttcc gaagagggag
    12901 cggtcgaagc cgagctcatc gatgatcggc cacgcccagt ctcgggtttt cgggttgtag
    12961 agctgggtgg tcgaagcgtg ggagcgctca ttcttcatca ctccggtcag ccagtaggca
    13021 agcagatccg gcagggccag atagtgcttc gccgcggcga acacctcccc ccggtcccgc
    13081 ttgtgcgcca cgagctgata gatcgtgttg aacggctgga aggcgatccc ggtctcctcg
    13141 aagacccgct cctttcctcc cagaagcgca ctcacttcct cgatcaggcc gtcggttcgc
    13201 ccatcgcgat agtggtagca gagcgaaacc aggctcccgt tcccgtcaac cagggcgtaa
    13261 tccacgcccc acgtgtcgat tccaatcgaa acgatctcat ccggatagca gtcaaacgcc
    13321 ttcttcaaac cggtcttgat ctccgaaaac agtccctgaa tgttccagta gaactcaccg
    13381 agaatctgat cgttgtgcgt cgtgaagcgg tgaaccactt cgaactcttc caaattccca
    13441 acgagaaccc gcccgttgct tgcccccaaa tcaatcgcga tgcgcttctt catctttaat
    13501 cctcaatcct catcgtgcaa tgctccatga gatatgcctc ggcttcggca ttccacagac
    13561 cattatgctt tttcaccagc tcgtccagtt tggcgaagag cgggtatttc ttccccttct
    13621 ccgcaaaatc ttccaacgcg gtgagcggga agtcaaggtg tgtgtagatc atcttctttc
    13681 cgccgggaat cttcggaagg tcgatcaccg tctgaggaac cgaggaaagt ccgccgatgt
    13741 gggtgaccat tccggcggga ttgatcaagc cctgctccat cagcttcaac gactcgcgca
    13801 ggtcgtcggt gtttccaccg ctcgttccga cgatatgggt cgaagcgtag tgcacgttgt
    13861 agaagttgag gcttgcggta aagtcggtcc gctccgggcc ggcaaagaag ttcaggcatc
    13921 cgtccttggc gagaatcgca tccgcctgct cgatcagggc ccgaaccggg gccatgacga
    13981 acacgtcgtc gtacccttcc ccgttggaaa gctccatcaa cacgctgtcg gcatcttcaa
    14041 gatcccgggt gttgaggtag tgcagctcga ccccgttctt cttcgcatgc tcgacggtat
    14101 agagcatggc ggctcgatcg aggcgctcct gattgatatc ggtgacgacc agacgaccgg
    14161 gccggcggtc gggattgttg atcgcatagt cgatcgcgcc catccccatc ggaccgacgc
    14221 ccgccaggat cgctgcgttc ccgccttcga cgatccccat gtggtggacg tacgaaccgg
    14281 cggtggtgtg gtacatcgca tggaacgttc cgacgacgca gctcaagggc tcggccaggc
    14341 ttccgaggaa gtaggaagga ccggcgtacg gaaggacgca gtccagctcc atcagcatgt
    14401 ggggaaggat gacgtaggtg gcatcccctc cgatatacgg gaaggagtac ccgggggcat
    14461 ccagcgttcc tttgtagttc atcgccggct ggatcgtgaa ccgcattccc tcgtggtact
    14521 tgtctttcca cttcttcccc acctcgatga tctccccgca gagctcgtga ccgagcatga
    14581 ccgggttttt atcgatatct ttgggaatcc gcttatggtt cggcccctgg gcagcggctt
    14641 tatggtcgct catacagatg ctgttggtga tgaccttcgc caggatttca tcatctttga
    14701 ttgcaggaag ctcaaactct tcaatccgca tgtcgttgat gccatacagt cttactgctc
    14761 gtgttttcat cgtaattctc ctaattcaac atgacctgtc caccggtgac cggcaccgcc
    14821 tgcccggttt cataggtctg ttcgactatg taaaagatcg ccttcatcac gtcggccgtg
    14881 gtgcagcccc gattcatcgg cacctttgct tcataggccc gtcgtacatc ttcaatcgtt
    14941 ttggcccccg gaaccttgtt cgtctcgagg tattggacga acagtccccg cttcggatcc
    15001 gaccaaagcg gaccgtcgag gaagtttccg ggacacactg cattgacctt gatattgtct
    15061 tcgaccaact ccagagcaaa gctctgcgtc agtccgatcg tcccgaattt cgcaccggca
    15121 tacgccccgt tcttgttgga tccctccaaa ccggatttgc tgctgatcgt cacgatgtcg
    15181 gtgtaataag ctcccgaggg gatgttctgg atcgccatcg tccgggaagc gaacttggtg
    15241 cagatgaaga atccggtgta gtccacatcg gtaacgaagg cgaaatcctt caggctcatc
    15301 gtcttcaccg aaccggctct caggacgccc gcgttggaga tgaagagatc gacgcctccg
    15361 acctcggaga cgaccgtgtc catcatcgcc tggacggaag cttcatccgt cacgttcact
    15421 tcgaccgcct tcgccacggt gatcatcgca tcgtagttca gttcggaagc aagcgcttgc
    15481 gccccttccc gattcaggtc ggcgacatag acgaacgccc catgatcgac gagcgagcgg
    15541 accatcccct cgccgaatcc ctgagcgccg ccggtgacga cagcgacctt gtcgcggacc
    15601 acgtctgatc gggtaagtgc aggcggaagg gtcggcagct tgctcacccc ttcaccccgg
    15661 accgcttcat cgtagttcgt ccccagcccg tacctgagct ctccgacgcc aaccgtcgcc
    15721 ggatacaccc gctccttctt cacgtactcg tcgaagaggg cttgccatgc tttggcggtt
    15781 tccttcatcg agtccccttt ggaaggggaa ggcagcacaa gggcgcttgg ccccggcccc
    15841 agcgtgtgga tgatcaggcc gagggagccg tcaaagctca ggccgcggat caaaggagga
    15901 atctctcttt tgatatcttc gaagaccgtc atgatctgac ctcctcaatc atggtatagg
    15961 ggttatggac atcgctgttt ctggcgatcg ccgcatagcg ggcgatccgc tgattcagcg
    16021 cttcatcccg gtagggattc tcagggtgga agagcccgta gaccggatcg accgaggaga
    16081 gcttctcatg ggctacgaga gcccaggcgc tttcgatcag gtgcctggcg ttctcctcca
    16141 aaagctcggg acaggagaag ctttgccggg aggaattgat gtccaccccg ctgatctcca
    16201 tcaggttccg ctcctgagcc agcctttgga tgcgcgcaag ctgctccttc gtgtttctcg
    16261 gcggcatata ggtgatcgcc gggaatccga tctccacaag gagatcgagg agctcgtcaa
    16321 ggaacccgtc ctcgaactgc tccgccttct tgtccccggt cggactggag gcgacgtccc
    16381 cgagatacgc gtacgcggga atcgctccga ccgatttggc gaaggcaacc gcctcacgca
    16441 catcgatcga ctcctcccgg gagggctgga tgaagaacga cggcaaaaat gcgctcttca
    16501 gcactcccaa gagatcgtac tcgtagtgcg ggttcgcacc atcgagaagg aacttctcca
    16561 ccttcggagg aatgtccagc ttcagctcgc agcggaggaa cctcaggagg gtctccccct
    16621 ttccatagga agcgatcagc ttcttggaga gcgcatggag gatgtgacgt tcggtgatcg
    16681 atcccccttc gccggcgcgg gagagcggaa cgacatcccc ttcgaaatcc acgacaatcc
    16741 cgtatcgttt caggagggtg ttcagtttcc caacctgctt cctgttccgt tcattccgga
    16801 tcgcctgcaa gggagcaaga aaggccttca cctcctcgat ccggccgctc ggcaccccgt
    16861 ggatgcagat gtagacgatt ccttcgctgt cggggttgtt gatcttcttc ttcgccaagg
    16921 gggtgtccag gaaactcacc ctcagctcga agccgaccgt cgaagcgatt ccgatcagac
    16981 tcgccgcctc gttcatctcc tcggccgccg agatcgagtc atgatcgacc gatccgacga
    17041 tccccaagcc cgcctgccat gccgcaaacg cggcggcggc aggctcatag ggactgaacg
    17101 agtaggtggt atgaacgtgg ttgttcacct cgagggaggg agtgtttgca agcacccctt
    17161 cccggattgc tttccccaat accgcggcgg cggcaaggcg aaccgacttg tcaggatcgt
    17221 tgatcttgat cttggcatcg gaaactgtca ccctagaccc cctcgctgct ggtgcggtag
    17281 aagtggctgt cggtatagtt ctgcttcacc acgtggttcg gcagcaccat gatctgctca
    17341 tggatcgcat cgaggatcga ggaagcctga gcaaagtagt actcctccat ctccgcaggc
    17401 ggggtaatcc agttcttcga cccgacgacg accgccggtc cgtcgaggta ctcgaacgcc
    17461 aaacggttga ggttcgaggc gacggtgtgg agatagctgc cccgttcggc cgaatcggtt
    17521 acaagaacca cttttccggt tttcttcaca ctctcgacca gaagctcgta cttcaggggg
    17581 ttgatccacc tgaggtcgat cacctcggcg tcgacgccca gctcggcaag cttctcgctc
    17641 gccttcatcg cggtgtagag ggagggtcct aaggcgatca gggtgatgtc ctttccttcc
    17701 ttgcggatcg ccggctcccc ttcgggaatc tcatagtatc cctcgggcac cccttccttg
    17761 acgaactgct ccccgattcc gtagagcttc tggctctcga agaagacgac cgggtcggtt
    17821 ccccgaaggg cgtagttcag catccccttc acatcatagg gggtcgccgg gtacatcgcc
    17881 ttgaggccgg gcaccgagcc gaccatcgcg gtccactcct gggagtgctg ggcgccgtac
    17941 ttgtttccga cggagacgcg gagaaccagc ggcatcgtca gctggccacc gctcatcgcc
    18001 tgccacttgg gcatctggtt gaagacttcg tctccggcgc agccgaggaa gtcgcagtac
    18061 atcagttcga cgaccgcccg gccgccggca agggcatagc cgacgccgct tccgacgatc
    18121 gccgcttcgc tgatcggcga gttgaagaac cgggagggag gaagcagctc ggtaagaccg
    18181 cggtacgcgg cgaacgcgcc gccccagtcg cggtggtctt cgccgtaggc gaccatcgtc
    18241 ggatcgatcg caaagcggtg gaccatcgct tcgaagaccg catcgcggta ttgatagagg
    18301 cgggccgccg gatacatctt tccttcctcg tcatacgcat agcggctgcg cttggcgatc
    18361 tgctgaaccc gggggttctc ctccagggtc tgcagcaaag ccggctcggt cgtgtcgaac
    18421 ttctcgacgc ttccgttgct gaacatcacc gattcgatga acgcttcatc gaccatcgtg
    18481 ctgatcgcgg gatcgacggt gatctcgagg gtcttgcgca tcttctcttc cagaagggcg
    18541 tcgtacgatt caagctcgct tgcggatgcg atcttgttct tcaccagata ctctccgtac
    18601 gcttcgatcg gatcctggtt cctgaacgca tccagctctt ccttggtccg ataggtcatc
    18661 gcgtcgctgg ggctgtgccc gctgtagcgg taggtgatcg tatcgagcat cgccgggcct
    18721 tttccttcca gaaggagggc cttcttcctt gccgtcgcat ccgccacggc gagggggttg
    18781 aagccgtcga cccgctcggt gtgcatcgag ttctggttga ttcccgcccc catccgggct
    18841 gcgattccgt agcccatcgt ctcgccggag ggctggccgc ccatcgcata gaggttgttg
    18901 aagacgttca caaggaacgg cggatacccc gggttgtcgt cccagagggt cgtatactgg
    18961 tccatcgagg caaggacgag cgcttcatag accggaccgc gggcaagcga accgtcgccg
    19021 atattggcaa tgacgattcc cttcttgcgg ttgatcttct tgtagagcgc cgctcccacg
    19081 gcgatcgttc ccgaaccgcc gacgattgcg ttgttcggca tcgagccgaa gggcttgaag
    19141 aaggtgtgca tcgagccgcc gaggccggcg ttgaagccgt tcttcttcgc atagatctcc
    19201 gcgagcgctc cgtagagcac aaagttctcg gcaagatctt ttacggtttt gcccttgtgg
    19261 tgcttttcga cgaccttgaa ggtgtttccg cccatatagc tctccatgat cgaaagcagc
    19321 tctttttcac ttaattgggc gatcgcgctc atgctcttgg cgaggatctc tccgtggctg
    19381 cggtgggagc cgaagatctg gtcatccacg tcgaggacca tcgcctgccc gacggcggcg
    19441 ctttcctgac cggcggagag gtgggccgga ccgaggtgct tgtaggagat tccctgatac
    19501 acaccctcct tcttgatcat gtcgagcatc gtctcgaact tgcgaatcag gagcatatca
    19561 tagtaggccc ggatcaggcg ctccttgcca taggttttga gctctttttt tacatcgctc
    19621 acatagtggt tgatggggat ttcaggcaac acaagggtgt ctttcttccg caacgttgcc
    19681 ggatcaaatg gaatctgtct ggacatgacg tatctctcct tctctcttac aggctcgaat
    19741 cgagcgcaaa tactgcatct ttcaacaatt cactgacact tgggtggggg aagacgatct
    19801 ccttgacgtc ttggatccga agctccgcct ccagaatcgc ggcgacgccc caaatgattt
    19861 cactgctata gggtccaagg aggtgaacac cgagaatcac cccgctctcc ttatcggcga
    19921 caaccttgat catcccggga gcgcgcttgc cctgctcggc cagaaagcgg ccgctttggc
    19981 gcccttgggc ggtgtggaca agcacttcgt agccccgctc cttcgcctgg gcttcggtca
    20041 ggccgcagcc ggcgatctcg ggattggagt agacgaccca ggggatcgcg tcatagcgca
    20101 tcttcattcc cttgccgaag atcgtcgaaa cggccacatc ggccatccgg ctcgcactgt
    20161 gggcaagaag tgatttcccg ttcacgtccc caatcgcata gaccgtcgga agactggtcc
    20221 gcatttgttc atccacgacg acggcgccct tttcgatctt caagcccaag gcctccaacc
    20281 cgccgatgtt cggtcgacgg cctacgctca tcaggatctt ctcggcggcc agctccttcc
    20341 gctcgccttt ggcgtcggtg tagaccacgc tcttctcggt gatcttctcg accttgcagc
    20401 cgaggttgaa ggttacgtcc ttcatcgctc tccgggcgag cttgccgaac tcggcgtcgg
    20461 ccatcggaag gatctcatcg agcatctcga taaccgtcac cttcactccg accatcgaga
    20521 agaaggaggc gaattcgact ccgatgaccc cacctccgat gacgacgagc tcggagggaa
    20581 ggctctcgat ttccagaata tcatcgctgg tgagcacatg cggcagatcg gctccgggaa
    20641 tcggcggaac gaacgccgac gatccggtgg cgatgatcag gtaggaacat tcatggatcg
    20701 ttccgttcac ttcgacccgg tgggcgtcga gcgctttcgc ttcgccgaag acggtttcca
    20761 ccttggcgct cttcatcagg aactcgattc cccctcgaag ggtctcgacg gtatcctgtt
    20821 tatgggccat cgcgctcttg aggttgaagg cgatatcctt tgcatcgacg ccgaaaagcg
    20881 atccgttctt cgcatgctcg taggtcttgg cgctgccaag caagctcttg gtcgggatgc
    20941 accctttgtt ggtgcataca ccgccgtagt gctccttctc gatcaggaga accttttttc
    21001 ccaatgttcc ggcgcgctgg gcggcgatgt atccgcccgg gccgctgccg atcacgatca
    21061 aatcatactg tgccattgct gcccatcctt atcagaagac gatcgagact tgcgattcga
    21121 tcgatcagag cgcgcaggaa gcgcgccgca tcggctccgt ccaccgcctg atggtccatc
    21181 gtcagcgaaa gaccgatgtg gtcgatgaac tcgacgccgt tcttggtgga gaccggcttc
    21241 aggacgatcg tcccgacgcc gagaatcgcg acctcgggga tgttcagcac cggggtgaac
    21301 gcctcgatgc cgaaggaacc gacgttggag acggtgaagg tcgatccggc aagctcatcc
    21361 ggctgggcca ccccgtcccg ggcccgagtg gcgagcacct tggtccgggc gctgagctcc
    21421 ttcaggctca tcagatccga accgaagagg acgggaacca tcagaccctt ctccgtatcg
    21481 gtggcgacgc cgaggtggac atgctcgaaa aagcggatcg tttcaccgag gtagtgggcg
    21541 ttgaacgcgg ggaactcgag aagcgtcctg acgacggcga agttcaccag gtccccgatc
    21601 gtgatgccgc tcgtctcctc atcctccttg aatgccgccc gcagcgcctt caaggcccgg
    21661 gcgtccgccc acccgtggag actcagctga ctggtcgtct ggagggactc caccatccgg
    21721 cgggcgatca cttttctaat cccgcggacc ggataatcgg tgtgggcatc aagatcaaca
    21781 accactcgct caggagtttt gctcacatca cgggcaagca cccggccacc catgccgctg
    21841 ccgaccgaag gaggaacgac tccttccttg agggcctgcg ctttcgccgc cggagacagg
    21901 ggagatccgg cgagcgcccg gatatccttt tcgacgatcc gtcctttcgg tccgctcggg
    21961 gtcgctgcgc tgaggggaat ccccagttgc tcgctgagct ttcgagcccg gggggtcgaa
    22021 ccgccggcag ccggtgcgct cacaggcgca ctcgccgttt tcggctcttt ctttacagtt
    22081 tcaggctctt caacctggac gcttttttct tcttctttct tcttgggtcg ttgaatctca
    22141 aacgattcgc cttcctctcc gacgatgaga atcggctcca tgacgggaac atcatcacct
    22201 tcctcccaga gccgggcgag aaccacccct gctgcggtgg attcgacgtc gatcgtcgac
    22261 ttgtcggttt ccgctgagag gagaatgtct ccgactgcga ccttgtcgcc gacgttgacg
    22321 ttccattcca cgatgatgca cgattctacg gtgttgcctt gtttgggcat caatacctgt
    22381 tcagccatcc ttttgctcct attgtacaat caggacttcg acgccgaggg tttcaagctc
    22441 ggagacgaga tcccggggaa atccactatc ggtgacgatc gtatcgacgt cactgaccgg
    22501 catgattcgt acaaagcccc ggttgccgac tttcgatgag tcgacgcaca ggacccgccg
    22561 ggtcgcttgc gcgcacatcc gttggacgac ttgggcgttc tcgaccaggc tcgtggtgag
    22621 accgtgggca agggtgagcc cgtccgttcc gaagaacgtc gtctggacgt gataactttc
    22681 aatatgggtg atcgcggcgg gcccgacgag ggcttccgct tcagggcggt attcaccgcc
    22741 tatgagggtg atgttcaaat ggttgttcgc ccggccgtac ggcaagagga gcgtagagtt
    22801 cgtaacgacc ttgatcgatt gctttccgaa gaggtagcgg gggatcaggg aacaggtcga
    22861 accgttggtg atcatgatcg aatcgccgtc cttcaccagc ttcgccgcag ctttcgcaat
    22921 cgcttccttg attttggtat gggcgtcgtt tcgcatcccc acctggggag tggtcgccac
    22981 caccgctttc ccatggcttc ggacgatcag gccttttgtg tccaaactcc ttaagtcgga
    23041 acgaattgta acggccgaga cccccaactc ctccgcaagg cgggtcaccg ggtattcctt
    23101 gccgcttctg agcaacgtga gaatctcttc ttccctcgga ctcaagccga acaccttttc
    23161 gctccttgtt tctttttact ttcggaacgc tttcgattct atatcacatt gaactaatat
    23221 gcaagagata tccctatata ttttcttcca tacgagtctg tcatgaatat tctgctatgg
    23281 caggtaaaac acctccctca gcggaatcga gatgggactg ttgtcctcgt tggtctccat
    23341 gatatccgcc atatacgccc accacttttg gacgatcggg ttttggccaa gatcctgaga
    23401 gccttggccg gtgttgtgct ggaccgcgaa gagtttgccg gtttctttct ctaggaagat
    23461 cgaatactcg ctcaccccgc tctctttgag caatgcggcc agttcaggcc agatctcgct
    23521 gtgtcgtcgc tcatattcct cttcatgtcc ctttttcagg tacatcacga acgcttcacg
    23581 catggctccc tcctatcgca tctcatgctg cttgcgcagc ttgcggttgg ctttgtacag
    23641 atcaagcagg cggggaagga gaaccgcgat gatcagaagc gatccgatga cgatcgtcat
    23701 caacgttccc gagatcttca tcaaccccat cccgaacttc agatacccga cgaggagcga
    23761 tccgaggaac acgccccaca ccgttccctt tccaccggtg atcgaaacgc cgccgagcac
    23821 aaccagagtg atgatctcca tatcccaccc agtggcgatg ttggagcgaa ccgagaggac
    23881 tcgggatgtc aacagaatgg cggtaaggcc gctgacgaag ccggtgatcg tgaagttgat
    23941 gatcctcatc cgcttgacgt tgatcccgct gaagcgtgca gcttcggcgc tgtttccgat
    24001 cgcatagagc cgccttccat aggtcgtctt gtggaggatg aagccgaaga tcaccatgag
    24061 cacgaggtag agcacgaact cgaaggggat cgtggtgcct ttgatgaact cctgaccgaa
    24121 aaacccgtag gttttcggaa acgtcgttcg agcctggtcg ccgagcattc caatcgcgat
    24181 tccccggtag atcgattgcg tcgccagtgt cacggcgatc gccggcatct cgaggaacgt
    24241 gaccatgaat ccgttgaaaa accccgccag cgtacccacc gcaagggcga tgaggatcag
    24301 cgtcggaatc gaagccccgc cggccgctgc gttgcccacc gcgtacgcgc agagagcgat
    24361 gattgaggcg attgaaatat cgatgtcgcc gcacatgatg atgaagatca tcgggagggc
    24421 catgatcgcc ttctcgctga attggaaggt ggcgttcatc aggttgaacc agttgagaaa
    24481 gtaccgggtc cgggtcgaga agaagatgat gatgaggatg aagatgatcg acagcaggac
    24541 ttcccacatg gtgaagattg tgatcaatcg ttctttcagg gaactttcgt cgatgagtcg
    24601 ggtttttgcc atcagctcgc gtttttctct cattacgatg ccctcctctg tgccaacagc
    24661 ttggtatgct ttctacggtc gctgagggtg ttcagggtga gcgcggtaag aatgatgagc
    24721 ccttggacga aggtctgcca gaacacggaa agatagacga ccggaagggc gttggtcacc
    24781 acccccagga acagcgtgcc caagaccact ccggtgatcg cccctgcccc gccggcgaag
    24841 ttgacgccgc cgagcacgca ggcggcgact gcctgcatct cgaagccggt ggccatctcg
    24901 ttcaccgccg acgcgtagcg ggcggtccac agagctcccg ccagcccgct gagcgtcccg
    24961 ctgatcgcaa agacgaccac ccggattttg ctctcgttca ctccgacgaa cttcgcggcg
    25021 gtgggatttc ctccgatcgc atagacttcc cggccggttc gggtgaagcg gctgaagtaa
    25081 tagaagaccg cgatgacgat gaacgctatc cagaggagca gcgatatccc taggaaggta
    25141 acccggggga acgcgatata ggaaggagcg agctcgtggg cggtgaccca ctggtttccg
    25201 ctcaagagga acgtaaggcc ccggtagatg cttaccgtcc cgagggtggt gatgatcgga
    25261 gggatcttac cgtaggcgac gaggactccg ttcacaagac ccatcacaag tccgattccc
    25321 attccgacaa acagaagcgg aaccgcgccg aacgcctgat tcgcttcgtt gatcaggccg
    25381 cacgccatgc cggtgaacgc gacgacggag ccgaccgaca ggtcgattcc gttggaaaga
    25441 atcacgaaga actccccgat cgcgacgatc gcaaggatcg ccatgtcgtt gaggatcgtc
    25501 gagatgttcc gtacggaaat aaattgcggc gagcggatcg tgatcggtat cagcagtacc
    25561 gccagcaata tcaccaatgc agcttcgcgg cgggcaagga tcttctttat cgtattactc
    25621 ggcattgtcc atctccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctt
    25741 cgcggcgggc aaggatcttc tttatcgtat tactcggcat tgtccatctc ctccgttgaa
    25801 atcgcccagc gcatcaccga ttcggcattc gcttcgcttt tatcgagaat cgcggtctgt
    25861 ctgccttcat gcatcaccag aatccggtcg ctgatcccga tcacctcggg cagctcgctt
    25921 gaaatgagga tgatcgccac ccccctcttc gccatctcgc agatgaactc atgaacggca
    25981 gctttggtgg cgacatcgat ccccttggtc ggctcgtcaa ggatcaggac tttcggctcc
    26041 gtgccaatcc atttggcaag aacgactttc tgctggtttc ccccactgag ggtctcggca
    26101 tcgacgtgat agccggtggc tcggatctcc atccgctggc cgtggtcaag cacataggtc
    26161 gcctcttctt tcttccgggt gatcgaacgc ttccacgaaa gggcgtcaag gaccggcaag
    26221 gagatattgt gcgtcaggct catcttcagg atgaggcctt gcttctggcg gtcctcgggg
    26281 accaacgcga tcccccgcct catcgcatca tacgggctct tgggcatgaa gggctttcct
    26341 tcaaaggtga tcgttccgct cgaagcctta tcgactccga aaatcgttcg aaccacttcg
    26401 ctgcggcccg ctccgatcag gccgaacaga cctaaaatct caccctcgcg gagggtgaag
    26461 gaaacgttcc ggaacgctcc caattggcta agcgaatcga cccggagaat ctcctttccc
    26521 caattaggga catggtcggg atagagctga tcgagcgagc ggccgatcat cattcgaatg
    26581 atctcatcct cgcttgagcg gtcgattcgg ccttcgccga tatattgacc atcccgaagg
    26641 acggtgtagt agtcacaaat cgcaaaaatc tcactgaact tatgggagat gaaaagaatc
    26701 gccttcccct ccgctttgag ctgacggacg attcgataga gatcctcaac ctcgcgatcg
    26761 gtaagggcgg aggtaggctc atccatgatg acgagcttgg cgtcaaggct gagggctttg
    26821 gcgatctcaa ccatgtgccg ttgggcgacg ctgagattct ttaccaatgt atcgggattg
    26881 attgaaagct gcattcgggc cagaagtgcc tgcgtctcct tgcgcatctg cttccaatca
    26941 agctttcgag ttttaggatg gcgcacgtgg tgccccatgt agatgttttc cgtcacgctg
    27001 agctcgggaa acatcgaagc ctcctggtga atggcgacga ttccggcttc ttgggcgtcg
    27061 atggcgtttc ggaaggtgat ttcctctccc tcaaggaaca cgctcccgcc ggtgggctga
    27121 tacactccgg tcaaaatttt tacaagggtg gattttcccg ctccgttttc tccgatcaac
    27181 gcgtggactt ccccggtgcg aatctcgatg cggacatcat ccaacgctcg gattccggga
    27241 aatactttcg tcaggttctg gaccttaacc aatggctggt tcactcgttc ctcctcacat
    27301 caagaacaac aaacatcgat cgggctcccc acctcgtgaa gaacccgatc ggagtacctc
    27361 ttagaagtac gcagcgaaca tctcgacgtt gtctttattg aagacgtacg gggtgctcat
    27421 gaaaatgata ccgtcctcga gaactttcat gtttcccatg cgaccggcgg gaatctcttc
    27481 accgacactg cctttcttcg tgccgtcgat gagggcattg agcagatagg tggaggtgta
    27541 tccgagatca atcgggttcc acaaggccat ttgacggcag gtgccatcga ggatgtatcc
    27601 cttcatttcg gaggggagtc caagaccggt gacttcgacg atgccggctt ttccttcatc
    27661 cttcaccgct tgagcggttg ccaaaagtcc aacggtggtc ggacagatga tcgccttcag
    27721 gttcgggtac ttcttcatca gagcgacggc ttcgcggtag ctcttgtcgg gaaggtcatc
    27781 gccatagacg acttcaacaa gcttcatctt cgaatacttg ggatccttga tctcttcctt
    27841 catccagtcg atccagaggt tctggttcgt cgcctgggcg ctggcggaaa gcacagcgac
    27901 gtctccggcg ctgttggcaa gctcggcggc aagctgaatc tgctggcggc cgatcaactc
    27961 cgcattggag ggaagcaggt cgacgattcg tccaccgacg ttgattccgc tgtcaaagct
    28021 gatcaccttg attccggcag ccatcgcttt ctttgcaacg ggaatgaggg cgtcggcgtc
    28081 gttcgcgctg atcgcgattc cgtcgacgcg ttgggcgatc aacgattcga tgatctcgat
    28141 ctgtccttca gcggtcggct ggctgggacc catgtacacg gtcttgattc cacccagttc
    28201 ggcggccgcc tcctcgcttc ccttgaacac agcgtcaaag aatccgttac ccatgttctt
    28261 cacgaggata acgatctcct tctgagcttt cgcgtcttcc ttggttccgg cagcaaacag
    28321 ggtgccgaag gccaagccga tgataagaat aacaaatagt gttttcttca tctttgttct
    28381 ctccttttct ttatagttct atcttctcct gatattcagg ccgactcacg tagttacttt
    28441 tgcccgatat tgctccactt cccaattccg aatgaattcg atcatctcat cggtcatgaa
    28501 cagcggtccg ccgaagctct cgctgtacac gctgatccgc accgtgtcga tgaacagcat
    28561 aaggggcttt tcgccgtcgg cgaacacccc ggcatcctgg acgcagacca ccttggggtt
    28621 ttgtccgtgc ttctttctaa aggcttcaaa cgcttcggtg atcgaatctc ctttaggcac
    28681 gaagagcggc ctgaaccccg aatagacgat gtggtcgggg gtgaaggagg atgagaccgg
    28741 ataaaagctc tccgcgcttt cgagcaacga cctcgtgtcg ttggaatgga aatatgcgac
    28801 ctctccggca aagagagtct tgagctcctc cttgatcgga tcgacgatcg tcgaatcgat
    28861 ttccaccggt ctttcatcca catcccgttt gaggttcgat ttcaacgtgg aaagcacatg
    28921 ccggtagagc tcgtcgatct cctcaaggct ctcccctccc acaaagatgc cgtggttctg
    28981 aaggatgatc aggttgggga cgctctccaa gggcatcatc gccctcttga tcgtgtcggc
    29041 gagaatgaag ccgggtttca ccagatcgat ccagagcgcc tcgggaaaga gctccctggc
    29101 gtattttgct ccttcgaccg cacaggtcag tccgttgacg agcgagggat gcaggtgcac
    29161 gacgtaggta aacggcaaga gcgcatggag cagcgcttcg acggaaggac gggcggtctc
    29221 cccttcgagg cgcgaggcca tcatcacctg cagcacttcc gcttcccgct tggcatcatc
    29281 gtttgagaac ttttgattcc aaattgcttc cagcttggag cgatccattc gaacgaaccc
    29341 gccctcatca atcgatccga ggggatggcc gctcgccttc acatagagga tgtttccttc
    29401 tttgtatgag gtgtttccac ccccgagaag aacgagatga ggatcgcttc cgtaccgtcg
    29461 ggacgcgttg atcagggcct gcagactcat agccgttcct taagaattac ttgttcgtac
    29521 gctttgacgc gttgaatcaa ctccccttcc gacggaacat tgttccgccg gaggtactcc
    29581 gcccacacat cgccgaacgg cagcacggag aggtgttcac tgagggccat cgccgcatag
    29641 ccgttaccgg cctcttcata ttcaatcagt tgatcggtcg gctccagaag ggcgaagagg
    29701 agggctttcc tgagggaccg ggctccggtg acccatgcgc cgatccggtt gatcgaagca
    29761 tcgaagaaat cgaggccgag gtggcagcct tccagcttgc cgctgcgaac cagctcctga
    29821 gcgacccggc gggtcttgtc atcgaagagc acgacgtggt cgctgtccca gtgcatcggg
    29881 cgcgagacgt gcatcagcag ttcatcatca aacaggagga tcgaactgag cttgtcggag
    29941 acatcctc
//
LOCUS       DAOSLI010000021        27997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1891_length_27997_cov_27.437621, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000021 DAOSLI010000000
VERSION     DAOSLI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 27997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27997
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1891_length_27997_cov_27.437621"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLF67_04580"
     CDS             <1..378
                     /locus_tag="PLF67_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE89325.1"
                     /translation="VGTMNVTALPILHGGDEIFAYRIGSLGYVTDCSEVPQKSVEYLR
                     GLDTLVVDALRRFPHPSHFSLFEALGLISHVGPKHAYLTHLSHGFAHQELEEELPPSV
                     HVAYDGLVIPIGGENEKEKELYH"
     gene            350..3052
                     /gene="polA"
                     /locus_tag="PLF67_04585"
     CDS             350..3052
                     /gene="polA"
                     /locus_tag="PLF67_04585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOE89326.1"
                     /translation="MKKKKSFIIDGYGLIYRSYYGLGRMLSSNGENISAVYGFFTTLL
                     KLIREEEIDYISVAMDSAGPTFRHELYEAYKANRDAAPEDLHSQVPHIEAILKALGIP
                     VLEHAGWEADDIIASLAHEATKDGIHTVMFTGDKDLLQLITDDVSALRPARKGESFYR
                     PMGKTEVKEEFGVSVEQIIDYLALIGDSSDNIPGVRGIGPKGAAKLLEEYTTLEGVYE
                     ALEEQTPTMRKRLEENKEAALLSRRLVILDSTLYQEEGIDWEDYRVDRIDWANSIPLF
                     QELGMRTIVGELERLSGEKALKPLEKPKKDRRTYRGVTDLAELKSLFESAVKSGGIIA
                     FDLETDTVVELDATIIGFSFAWEAYTAYYVPLVHQGKTLIDEGGVKSLLRTYLEEGRL
                     KVVGQNIKFDYKVLKVRWNITIKHLYGDTMIAAWLLDAASTLNMDFLALRYLGVETLR
                     YTDIVPKDKTLAEIDYEKALFYAAEDADITLRLWQRFEELLKEADLLDLLFTLELPIL
                     RIIAHMELEGIFLDEGVIGPLEEEFAGRIDQIKAQIFELAGKEFNLNSPKQLQEILFV
                     EQGIPTGAKTRTGYSTASDVLEPLSGQYPLVALVLEYRELNKLASTYVEALPKQINRR
                     TGRIHPTFQQTGTETGRLSCRDPNLQNIPVRTEEGRRIRSAFVPRTGCVFLSADYSQI
                     ELVVLAHMADDPGLKEAFLGGMDVHRFTASLIFDTPMPEVTSEMRAIAKTINFGVMYG
                     MGTHALAQDLKITHQEAKRFIEQYFERYPAVRDFVEETKTRARKEEVVHTLSGRRRPV
                     GEINSRNAVERARAERIAVNTIIQGSAADIMKQAMIRVDRMMRERRLLSALILQIHDE
                     LVFEVPLGEVEEMKALVKEAMEGAVTLSIPLNVSISVGSNWGEL"
     gene            3049..3654
                     /gene="coaE"
                     /locus_tag="PLF67_04590"
     CDS             3049..3654
                     /gene="coaE"
                     /locus_tag="PLF67_04590"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269858.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOE89327.1"
                     /translation="MKPVVIGITGKACAGKNQYAKAFEELGARLIDVDRLGHQALEAN
                     KDRIIAEFGDSVADRGEIDRKALGAIVFSDPSKLKALEGISHPWMVARIERILAEETA
                     EVIVLNAALLSRMGLDRLCSHILFIAAPRCLRFRRCKARDGLSWRQFLKREASQRDIS
                     YQLLDPKAIHHRLWNWGSMEIIHRQVQRCCGRIGVKRCSRN"
     gene            3668..>4785
                     /locus_tag="PLF67_04595"
     CDS             3668..>4785
                     /locus_tag="PLF67_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89328.1"
                     /translation="MKKSIAIMLLVATSVVAIAVGIGIWYTRPSAPLAIIEEAKARQR
                     QPLLEVTPPQRAEVVPLPPDYQPLIDELLPLLQDELTQPKALSLEQQAYVDQAVENLR
                     RELLQSIDSQANRTYVDQQITSLKNELTTSLGRLEEKQVDRRAIIDSITADDLRPAFS
                     VFRDELLNQVRSEQPRTTVVMTGEVDEELLFKNLAWEIENNLDFYASYARDAILSTID
                     DDHMIALYESHRPMLLADLAASLPAPMTVIAAEPITGEQVLSELAREIENNKEFYATY
                     ARDAILDTISEDDLFSYYNHYRPLLLGDLYQGIKDEAKVEIADYADIIEQMEANREYY
                     LAQDRKALPGVLTEADILPLYATYRDDLAEDLFTLLGE"
     assembly_gap    4786..4885
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4886..6030
                     /locus_tag="PLF67_04600"
     CDS             <4886..6030
                     /locus_tag="PLF67_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89329.1"
                     /translation="ATYRDDLAEDLFTLLGEEIESNKGTYADYVRSAIGEGVSEEEVL
                     DILGREIETNKDAYAVRARDAILETISDDDLIAYYMQYRPALVADLAEELPAARAALD
                     REQILDDLYAEIETNKPLYAAYVRDALLETMIGDPEFDQVYEENRVDIDAVLLALGEE
                     IENNKKFYATYVRNALLDTISGEELVNLYLNYRDALIEVGVELDANQIMGILTNEIES
                     NREAYATWARKAIIDTMSDEELISYYLHYRPELLEDIYQGLKDRFAMASSAPVPSSPL
                     PATQRTVILQEAVEEEAAPPVVVPPSPSPAVQRTVILQEVVEEEAAPSVVVEEKPAPP
                     APRVPVSPSVTTKPTTVPYEMTPAEYETLRQEKRNEALDEILKKIG"
     gene            complement(6027..7076)
                     /locus_tag="PLF67_04605"
     CDS             complement(6027..7076)
                     /locus_tag="PLF67_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOE89330.1"
                     /translation="MNRDASITSLSALASKLTLSEEEKGFDESDGTLLPIKIPSSFFK
                     LINPDDPSDPIRRQVVPTVFEHTESRGETLDPLAEKRHAATARLIHRYPNRAAFLTTD
                     LCFIHCRHCFRRRFTGTFQGPANEEEIEQAARYVEKHPEITELLFTGGDLFTLNDVQL
                     ETMIGAFRSRRPDLIIRLCSRAPAARAERITPALIAMLKTFDTAPFFLMTQFNHVREL
                     TKEALRATALFIDNGIPAMNQTVLLKGVNDDADTLKELCNTLLANRIKPYYLFQGDLV
                     SGTAHLRVSIEDGMKLEAELRKRLSGLAMPLYALDLPEGGGKVPLVNSYVEEKKDGAL
                     IFRTPYGGHRSYPAD"
     gene            7174..8478
                     /locus_tag="PLF67_04610"
     CDS             7174..8478
                     /locus_tag="PLF67_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE89331.1"
                     /translation="MAKQKKGLKGYYLTTFLIGLGFFTMGLMDPLYDTYVPIFLSRFV
                     ESKTLIGAIMTFDNILAIFLIPIFSALSDRVRTPIGRRMPFIVVCLPVSAVAFGLIPF
                     SALSSLWFLIILLFILNLFKQAVRGPVVALMPDMIPADLRSEANGVINTMGGIAAIVG
                     TVGLARLMDVPINLPGRGPTKDILAFPISGILVIIATLLLFFFVKEKNAVAHDESERR
                     EPILSSLKAVFAEEDKSARAILFSLLFWFIGYQGILPFVGLYSTSILETSSGTASLAA
                     GMVGIAYAIFAIPSGIVAHKIGRKKTIRFALGVLIVLLLLIFFHDPLTSAMGKAFRQY
                     TFWALLFLFGIFWVSIVTNSFPMLWQMSTYRTVGIYTGLYYFFSQLASIISPPLTGAC
                     IDIFGFRSIFVFGSAAMGIAFFIMRNVQGGEPEDRKSEEMSK"
     gene            complement(8458..9012)
                     /locus_tag="PLF67_04615"
     CDS             complement(8458..9012)
                     /locus_tag="PLF67_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOE89332.1"
                     /translation="MNTVQLLFVSVSLSMDAVAVSLCKGLSMKRCSFSRAFPIAFSFG
                     FFQALMPLFGFGLGSLFGSFVERVDHWIAFVLLVCLGGITLYRSYAEKECHSAKEPFK
                     GEILLLSIATSIDAFVVGITFAILKINLVQAMSMIGVITFTLCLLALYLGKTMGTRLS
                     EHAERLGGVLLIILGWNILFTHLF"
     gene            complement(9108..9509)
                     /locus_tag="PLF67_04620"
     CDS             complement(9108..9509)
                     /locus_tag="PLF67_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89333.1"
                     /translation="MRKYRAAAILMIIHGGLMELAGLFMFLLPSTTGRTGIETGKYFS
                     FKLQYFQDNLAMMVIMGVIYGVLRLIGAIGLLKNRMWGLALSVINCVTTMILMMFMLP
                     AGIADGILAGSALVLILIQYFGDKNIVESRE"
     gene            complement(9520..10083)
                     /gene="efp"
                     /locus_tag="PLF67_04625"
     CDS             complement(9520..10083)
                     /gene="efp"
                     /locus_tag="PLF67_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOE89334.1"
                     /translation="MIKAGQIEKGTALLIKGQPFVVVEREFVNPGKGSAFVRVKLKSP
                     ATGQVLQETMKTQDNVEDITVADRDCQYLYNDGEHFHLMLTDTFEQLEVPMSSFPEYE
                     LLMQDGEVYRCTFWEAELLDIIIPAKVVYLVAEAEEAVKGDTVQGATKYVTTETGLSV
                     RVPIFIKQGEKIRVNTETREYLERVND"
     gene            complement(10080..11081)
                     /locus_tag="PLF67_04630"
     CDS             complement(10080..11081)
                     /locus_tag="PLF67_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HOE89335.1"
                     /translation="MQEAALFRSRTYHAIRSFFHERDYLEVDTPILSPTIIPESTIEN
                     FATRFRNPFLPSRDLYLLPSPEIYMKQLLAMGFGSIYQISHCFRNSEQLGRIHNIEFS
                     MLEYYSVGFDEEDSILLTEELFRRLEVPKRLQPPFRRMSVAEATKRYAGFDLEKTQRQ
                     PLLRQEALRLGLSVPDEPESWEETFNRIFLTFVEPNLPQERPLVLYDYPRRIECLAQV
                     NGAYRRRWELYGMGMELANCYYEESDEQKIEAYYRDEYARLIQTREISGGVIADVDPT
                     LAKTFARIGKVSGVALGLDRLLMGMMGRSSLEGVILFPLSAILSAGNRNLYHDEEYI"
     gene            11148..12476
                     /gene="mnmE"
                     /locus_tag="PLF67_04635"
     CDS             11148..12476
                     /gene="mnmE"
                     /locus_tag="PLF67_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOE89336.1"
                     /translation="MSRYRTDDIIYALATPWGSAALAIIRISGSGCHALLAPYFSKSE
                     ALRSAKNATLLHGYLCDQEGRRIDEVVLAVNTKGHGYTLEEAVEITCHGSLSVVAELL
                     DFLSLIGMRSAEPGEFTFRAFLSGRMDLTQAEAVHELVEAQSPVARAQALARLDGGLK
                     EKLNGMKNVLLALLAPVEVQLDYSEDELDEFVFPDELLDSLLNDLGRLLQSYAIGTLY
                     KSGARIVLAGATNVGKSSLFNLLLKEERSIVSPVRGTTRDYIEADLLLEGIPIRLYDT
                     AGLRESGDEIEREGIRRSEHLIGQADLVIHLLDSTEEGVDRAFDGRTIVVYNKADLRR
                     PPEGALAISAATGEGIGDLLSEVARRLKRGVEGVDESRIVIQSKRQADALLAAEAALK
                     GVKEDLQAALPLDIVAVGLNEALYHLGLITGEVTPNDILESIFSNFCVGK"
     gene            12478..14283
                     /gene="mnmG"
                     /locus_tag="PLF67_04640"
     CDS             12478..14283
                     /gene="mnmG"
                     /locus_tag="PLF67_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOE89337.1"
                     /translation="MDYHALVVGGGHAGIEAGLALSRIGFKTLLITQNLDAIGRLSCN
                     PAIGGLSKGNIVREVDALGGEMAHLIDRSMIQFRILNRKRGPAVQAPRAQADKYTYAR
                     LAKETLEGQANLEIFMDTVVDLIIERNRIVGVKTKRGHEISAEVVVLTTGTFMEGRIF
                     IGEYDAPEGRLEEPAAIGLGTAMRKKGFPVGRMKTGTPARVRKSSVDLDRLEVQEGDE
                     ESMSFSFDYDGVDRPSVPCHITWTNEKTHRIIADNIHRSPLYGGKIVGQGPRYCPSIE
                     DKVVRFPDRDRHQIFIEPEGLGTEELYLNGISSSLPEDVQSAFIHTIEGLEKAQIMRP
                     GYAVEYDYIDPQALFPSLESKLVENLFIAGQTNGTSGYEEAACQGLIAGINAAQKLKG
                     EKPLILGRNEAYTGVLIDDLVTLGTEEPYRMFTSRAEYRLNLRHDNADQRLTQKGYDV
                     GLRTEEHLQRLREKVSRIESVKDLLRIHRYREKSAFDALKMPEVTIEELAKSIPELGA
                     FPPSIRYQVELDVKYDGYIKRQERQINRFERFETMTIPPDLDYDTLEGLSTEARQKLK
                     HIRPLSVGQATRISGMRSSDAAILIIHLQRRSGDA"
     gene            14276..14932
                     /gene="rsmG"
                     /locus_tag="PLF67_04645"
     CDS             14276..14932
                     /gene="rsmG"
                     /locus_tag="PLF67_04645"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOE89338.1"
                     /translation="MRRYDRLLDEGLARLDLFFDPEQRSRLDTYIAELELFNPVYKLV
                     GAQGEELVINHIFDSLAGVKTIEELAKRYDHPTLADLGSGAGLPGIPLAIALPNLDVV
                     LVERMGRRVDFLRTALVVTSLHRRVTIIDRDIKELNERFEVLTFRAFRPLVEILDSVA
                     PLLQVGGVVVAYKGLRANVEEELKEVKRKVTSRWEEELIDLDVPFLERSRMLCLLHKI
                     "
     gene            14947..17478
                     /locus_tag="PLF67_04650"
     CDS             14947..17478
                     /locus_tag="PLF67_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOE89339.1"
                     /translation="MKKKAGVKKTRGAIIAALAAFIWAGILVLPSFGIELPFMEMIFF
                     LFSFSTGDNPLWLLPSALFFLVASIILIVNRKRPIVLAFQLFYSALVAYTLHSLSHIL
                     QDSPWPIFRTHLLERTEGVRSASFTLVVFFIGEILLLIIIVLISDAIRHRRRPRLEEE
                     RVDEPEETETVTFVPKARAEFPNVDDLPEYVDRTPPPPKPKREKAPAAEEISGENTVS
                     LAMFRRLSEEVKKREEQQNKPSIPSPAKGKSRSRVDEKQGAKELPGLMLAAVKRMEER
                     KEEKVVEEKPRSFFARHSEPSTLEIEEKVPSPPPPPQMPQPEEPAFAAPPVVEHSENR
                     VSVKIPPQVEQPELFDEDDPLESSRGIGGLDASALISKPKIGYRFPSESILRTYEKIG
                     DVIDEHTLRQGQLLVETLLQFNIQVDVTKIVRGPTVTMFEVLPAPGIRVGSIVSLSDN
                     IALALAATQLRIVAPIPGKSAVGIEIPNKKRDIIGFKEMLGSVTEKMKIPMILGRTIL
                     DEKRVIDLSLSPHLLIAGSTGSGKSVCINSLICTILYRRTPKEVRMILVDPKIVELNI
                     YNGIPHLLTPVISDAKRTLKAFDFCLFEMERRYNLLRALNVRNIVGYNEKIAANNLAR
                     EKLPYIMVVIDEFADLMHTVGKELETKVARLAAMSRAVGIHMVLATQRPSADVITGVI
                     KANIPSRISFAVTSLIDSRIILDEMGAEKLLGRGDMLLQTSDSPTMERIQGAFLSDAE
                     VEEIVTFATGQGEPEFIDESFFEDDPPPITESTVGLSDAITDEELMEQALQIVVERKG
                     ASASFLQRRLKIGYNRAARLVEQMEEEGWVGPANGSKPRELLRIP"
     gene            17479..18279
                     /locus_tag="PLF67_04655"
     CDS             17479..18279
                     /locus_tag="PLF67_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOE89340.1"
                     /translation="MYASRNGVIIPKEEAVVPITSREVQSGFYVYESLRVLGGFVTRI
                     NDHLDRLLASAKGIHLTHPFTKELIRLWVDELIKAEHIGDATVRITLYGGADPVLFIV
                     PVELLRYPDSYYRDGVKAVTFEGERLFPTHKSGGLLLNYVALEEAKRFGGFEALLVDR
                     NGRVLEGTRSNFFAFSNNTLHTADEDLVLGGITRSEVLRSARELGFTIDLNPIYLEDL
                     FAGRFTEVFISATSMAAMPLHTIDDLRFSAPFERTRALHDCVRARQWQ"
     gene            18387..19913
                     /locus_tag="PLF67_04660"
     CDS             18387..19913
                     /locus_tag="PLF67_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE89341.1"
                     /translation="MNFSELPLHEQVLTGIERTGFEQCTDVQQRVLPVTLAGKDVMVQ
                     SKTGSGKTAIYAITILQSFITAQADNREVKALIIAPTRELAVQIKADIDELAGGVNNF
                     RVGCFFGGVGYAKQDEWIEKGLDVYVATPGRILDYQKMKKVDFRQFDIFVVDEADRLF
                     DMGFYPDIQKMFSLLRPVKERQTMLFSATLSTKVRHLAWQFMNEPKEIEVQPEEITVK
                     EITQELYHISKDEKFRLLLQLLKQDEPVSCLIFTNTKARCVEVSKRLSMNGYQSKYLM
                     GDLPQRKRLEIIGWMKSGKLRFLVATDVAARGLQIDDLALVVNYDIPEDYESYVHRIG
                     RTARAGKSGKSITLADEEYVFNLEAIEEFIQMKIPVIWPEDLPEVEDKSASYTFRDLV
                     PRTGVRSSSARRGGTPSPSRGRKQAPRRAPERKQRRPRPAAPPKQHPPRRSAKAYADL
                     SALPLEERLAYYKKEYLGNQQPADEPVKKTSSPKPVDAKEEQAKKKGFFARLFANRGK
                     "
     gene            19913..21631
                     /locus_tag="PLF67_04665"
     CDS             19913..21631
                     /locus_tag="PLF67_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89342.1"
                     /translation="MLKRFVSYYRPYKGLFLLDLGMAVLSSILAILFPALTRHLLSVE
                     IPAGNLRAMVVIFVLMIAIYLIQTVSQYIRITWGHILGVKMETDMRNELFAHLQRLSF
                     GYFDRTKTGHMMSRITNDLFIIAEVAHHGPEDFLISLATIVGSYVLMFIYSPPLALIS
                     LIPFPFMVFYGSYFGRKMRATFRRVRSTVADVNSNVENSLMGIREVKSFGRETFQTEK
                     FQEVNNILKESKMQQYKVMGSYHALMGLFSNLYYFFTVVGGAILIMRGTIEYYDLITF
                     ILYVGIVLPPIDRLINFTEQLQQGMASFERFVQIMDEHPAIQDAKDARELTVKDGTIV
                     YENVSFTYENTDTAVLSDVNMTIPGGETVAIVGESGAGKSTFASLLPRFYEPQAGRIL
                     IDGTDITTVRQSSLRKEIGFVQQNVFLFDDTIRENLRYGKVDATDEQLWAALDAANLG
                     QFVRSLPLGLETQVGERGTLLSGGQKQRVSIARVFLKNPSILIFDEATSSLDTESEEL
                     ITEAFERLSEGRTAIVIAHRLSTIKNADRILVLDQGRIVESGTHQNLLQKNGQYAKLY
                     KNQDFM"
     gene            21646..22380
                     /locus_tag="PLF67_04670"
     CDS             21646..22380
                     /locus_tag="PLF67_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE89343.1"
                     /translation="MKALRITFAVIFVVPILVLSPLYAFFIAYLMEVTGMEKAAQNHL
                     RRCGSFIGSAILFFLGVKLVVTGRENLPETREGICYMGNHQSMLDIPAFVGPAKLWGS
                     VMAKVEVKKIPIINLWCKAMKIIFIDRKSPHDSIKAIFTGVEHLKSGGNLLIFPEGTR
                     SKTGKIGELKAGSIKLATRSRATIVPFAIKNLRPGFEGITNLKRVHASMAIGKPIETK
                     DLSKEELAGLHEVVYGAITDLFDQLP"
     gene            22343..23788
                     /locus_tag="PLF67_04675"
     CDS             22343..23788
                     /locus_tag="PLF67_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89344.1"
                     /translation="MEPSPISSINSLDVTNLSFSYQGWVGENPPPLFEKLSFSILRGS
                     KALLLAPFDSGKSTLGRMLVGGIPKYFPGRLEGAIKVMGHNLADIEVWNLLDVVTLVS
                     QNPQEQFVASTVEEEVAFGLESLGLPPKEMRRRVEDALTNWGLDELRQVSNSELSGGE
                     RKRVLLAAAEALDAPFWILDEAFDDLDQRWREHLARFIQTTDKTILLFSSRYLAQFDR
                     LFDHVFLLEDKQVRKVDPKQAATVFASLAGDTKPNPLSGRAKEYAHQHTLRVSNLVVE
                     RRRFSASSTETFHLQVPAFFLQSGEIVSLVGPNGSGKSSLSRVLCGLDDPLEGTRIID
                     GEVLDGSMLNRSVGYLFQNPDLQIFLPTVKEELSYSLRRVKGLSDEDISKRVAECADL
                     FELKLSHTPSTMSYPKRKALQAAVYYLLDRPFYILDELDTALTYHSALTMIRHLQRNG
                     AGILIITHDQQFITRTGGRIYQIEEGRVSER"
     gene            23785..24552
                     /locus_tag="PLF67_04680"
     CDS             23785..24552
                     /locus_tag="PLF67_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOE89345.1"
                     /translation="MTVNTYLSNTGWLYRFDPRAKIFFLALLLGWVFLPVTLTGLGCM
                     LLLILFTALSNPGPRFVRSTFTSILPMIIFMVLFAPFNVRSGKGLITIRSFVLLTEEG
                     ALQALRLAIRFISITYLATLFFATTPMPEILLALQWYRLPYNASLIITLAFTYIPFIA
                     DGFAQIEESHRLREAGAHLEQKRIGKIRSLIPTLTSSLVLALRSIPTLALSLELRGYG
                     RANKRTRYHDLSSYRRTFTDFLISGMIPVISFLVFKA"
     gene            24566..25078
                     /locus_tag="PLF67_04685"
     CDS             24566..25078
                     /locus_tag="PLF67_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOE89346.1"
                     /translation="MNKEKHALKVAVVAVLTAVVVVFTLVIRIPTTKGYLNLCDVAIC
                     FIAFTFGPVSAFLAAGLGTAIADLISGYAQWAPISFIVHGIEGLLIALIVRKQPLSKM
                     RTFLAAIVCILTVGLGYFILSAFFISTVAVAAAEIPPNMIQAGVGVVFGFALSKAIKR
                     AYPPVSALSW"
     gene            25091..>26042
                     /locus_tag="PLF67_04690"
     CDS             25091..>26042
                     /locus_tag="PLF67_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89347.1"
                     /translation="MYDRVAVIDYGKGDAVGLARSIRSRGIYSRIFPYTVSKERLAEC
                     KPKLIIHATDKEFERTNLAPEVTYPSTDLNEVLLSHVQANWTMERYAEDLILQIRETV
                     GRKNVLLGLSGGVDSSVCALLIHKAVGDRLKCVFVDHGLLRKDEGAAVKALFEETYHI
                     NLTYVHAEEVFLSGLKGVTDPEEKRKIIGKLFVDTFYEAGRKLGDISFLAQGTIYPDI
                     VESVQPGRTLAVKSHHNVGGLPEDLSWELLEPLSDLFKDEVRALGRVLGLPDVLLGRH
                     PFPGPGLGVRCIGAVTKERLETLKEVDAIFIDEIRKANLYD"
     assembly_gap    26043..26142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26143..26448
                     /locus_tag="PLF67_04695"
     CDS             <26143..26448
                     /locus_tag="PLF67_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOE89348.1"
                     /translation="KEVDAIFIDEIRKANLYDEIWQALATLLPVKSVGVKDGKRVYEE
                     VCTLRAVNSIDAMSASIYPFSGEFMNRTVLRILNEVPTVSRVLWDVSPKPPGTIEWE"
     gene            complement(26546..27499)
                     /locus_tag="PLF67_04700"
     CDS             complement(26546..27499)
                     /locus_tag="PLF67_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002681377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein RhuM/Fic/DOC family protein"
                     /protein_id="HOE89349.1"
                     /translation="MSNEVIIYKDGELELPVEVTPDKGTVWLRVEEMAELFDRDRSVI
                     QRHIKNIFKENELEQNSTCAKFAQVQNEGNRSVERIFDYYNLDMIISVGYRVKSQRGI
                     AFRKWATSILKEYIVQGYAVNDKRLKVLNKVIEIQSNIIADVLELDSKEVFDVIQKYT
                     QALELLDDYDHQVVQKPKPTNEESYQLSYNECRNLIDSMAFTTTSTIFGKEKSKGALE
                     GIIGSVYQTAFGEDIYPSTQEKAANLLYFIVKDHPFIDGCKRIAAAIFLYFLNKNNLL
                     FKDEEKIISDSTLVAITLLLAESKPEEKEIMINVIMNFLEW"
BASE COUNT         6350 a         7250 c         7762 g         6435 t
ORIGIN      
        1 gtggggacga tgaacgtaac ggcccttccg attcttcacg gcggtgatga gatttttgcc
       61 tatcgaatcg gttcgctcgg gtacgtaacc gactgtagtg aagttcccca aaagagtgta
      121 gaatatttgc ggggtcttga tacgcttgtc gtggatgcgt tgaggcgctt tccccaccca
      181 tcccatttca gcctctttga ggcgttgggg ttgatcagtc atgtgggacc gaagcacgcc
      241 tatcttacgc acctcagtca cggctttgcc catcaggagt tggaggagga gcttcctcct
      301 tcggtgcatg tcgcctatga tgggttggtc atccccattg gaggggaaaa tgaaaaagaa
      361 aaagagcttt atcattgacg gatacgggtt gatctatcgc tcctattacg gtcttgggag
      421 gatgctctct tcaaatgggg agaacatcag cgccgtctac gggtttttta caaccctcct
      481 gaagctgatt cgggaggagg agatcgatta catctccgtt gcgatggaca gcgccggacc
      541 gacgtttcgt catgaactct atgaagcgta caaagcgaat cgtgatgccg ccccggaaga
      601 tcttcactcc caggtgcccc atatcgaggc gattctcaaa gctctcggca tccccgtcct
      661 cgaacatgcc ggctgggagg cggatgacat tatcgcctcg cttgctcacg aagcgacaaa
      721 ggatggaatc catacggtga tgttcaccgg tgataaagac ctgcttcaac tgattaccga
      781 tgatgtctca gccctcagac ccgcccgaaa aggggagtcg ttctatcgac cgatgggaaa
      841 aaccgaggtc aaggaggagt ttggggtctc cgttgaacag atcatcgact acctcgcctt
      901 aatcggggac agctcggaca acatccccgg ggtaagggga atcgggccga agggtgcggc
      961 gaagcttctt gaggagtaca cgacccttga aggagtgtat gaggcgctcg aagaacagac
     1021 tccgacgatg aggaagcggc tcgaagagaa caaagaagcc gctctcctca gtagaaggtt
     1081 ggtaatcctc gattccacgc tctatcaaga ggaaggaatc gattgggagg actatcgagt
     1141 cgatcggatc gattgggcga attcaattcc cctgtttcaa gagctcggca tgcgaacgat
     1201 tgttggggaa ctggagcgcc tcagcggtga aaaagcctta aagccgcttg aaaagccgaa
     1261 gaaagatcga cgaacgtatc ggggggtaac ggatcttgcc gagctgaaga gcctctttga
     1321 gagcgctgtc aaaagcgggg gaatcatcgc cttcgatctt gaaaccgaca cggtcgtgga
     1381 actggatgcg acgatcatcg gcttctcgtt cgcttgggaa gcgtatacgg cgtattatgt
     1441 tccccttgtt catcaaggca aaacgctcat tgacgaaggt ggtgtgaaaa gccttcttcg
     1501 aacgtacctt gaagagggaa ggttgaaggt cgtcgggcaa aacatcaagt tcgattacaa
     1561 ggtcctcaag gtacggtgga acatcacgat caaacacctc tacggggata caatgatcgc
     1621 cgcctggctg ttggatgcgg cctcgacgct gaatatggat tttttggcgc tccgctatct
     1681 gggcgttgaa actcttcgct ataccgacat cgtacccaaa gacaagaccc ttgcggagat
     1741 cgattatgag aaggcgctct tctacgccgc cgaggatgcc gacatcaccc tccggctgtg
     1801 gcagaggttt gaagagcttc taaaagaggc tgatctcctt gatctcctct ttacccttga
     1861 gcttccgatt ctgaggatca tcgcccacat ggagctggag gggatcttcc ttgacgaagg
     1921 ggtcatcggt ccccttgaag aggaattcgc cgggcgaatc gatcaaatca aagctcaaat
     1981 tttcgaactt gccgggaagg agttcaatct taactctccg aagcagctgc aggagatcct
     2041 cttcgtcgaa cagggcattc cgaccggtgc gaaaacccga accgggtatt cgaccgccag
     2101 cgatgttctg gagccgctga gcggtcagta tccccttgtg gcgctggtat tggagtaccg
     2161 tgagctgaac aaactcgcct cgacctacgt cgaggcgttg cccaagcaga tcaaccggag
     2221 aaccggacgg attcatccaa cgtttcaaca gacgggcacg gagaccggcc gcctcagctg
     2281 ccgcgacccc aacctgcaga acattcccgt tcgaaccgag gaggggaggc ggatccgatc
     2341 ggcattcgtt cctcgaaccg gttgcgtctt cctcagtgct gactattcgc aaattgaact
     2401 cgtcgtcctt gcccatatgg cggatgatcc aggactgaag gaggcgtttt tagggggaat
     2461 ggacgttcac cgcttcacag cgagcctcat cttcgataca ccgatgccgg aggtaacaag
     2521 cgagatgcgg gcgatcgcca agacgatcaa cttcggggtg atgtatggga tgggaaccca
     2581 cgcgctcgct caggatctga agatcaccca ccaggaggcg aagcggttca tcgagcagta
     2641 ttttgaacgc tacccggcgg tcagggactt cgtcgaagag acgaagaccc gggcccggaa
     2701 ggaggaagtc gtccacaccc tgtcggggcg ccgtcgaccg gtcggggaga tcaacagccg
     2761 aaacgccgtg gagcgggccc gggcggagcg gatcgcggtc aacacgatca tccagggcag
     2821 cgccgccgat atcatgaaac aagcgatgat ccgggtcgac cggatgatga gggagcgcag
     2881 gctcttaagt gcgctgattc tccagatcca cgacgaactg gtcttcgaag tcccgcttgg
     2941 tgaggtcgaa gagatgaaag ccctggtcaa ggaagcgatg gaaggggcgg taacgttgag
     3001 catacccttg aacgtctcga tcagtgtcgg atcgaactgg ggcgagctgt gaagccggtc
     3061 gtcataggga tcaccggcaa agcgtgcgcc ggaaagaatc aatacgccaa agcatttgaa
     3121 gagctgggcg cccgattgat tgacgtcgat cgcctcgggc accaggctct cgaggccaat
     3181 aaggatcgaa tcattgccga gttcggcgac tccgtcgcag atcggggaga gatcgatcgg
     3241 aaagcgctcg gagcgatcgt tttttccgat ccttcaaagc tcaaagcgct ggaagggatc
     3301 agtcacccgt ggatggtggc tcggattgaa cggattctcg ctgaagaaac ggcggaagtc
     3361 attgttttga atgcagcgtt gctgagtcga atgggcctcg atcgattgtg ttcccatatt
     3421 ttgttcattg ctgctccgag gtgtctccgc tttcggcggt gtaaagcccg cgacggtctt
     3481 tcatggagac aattcctgaa gcgggaggcc agtcaacgcg acatcagcta tcaattgctc
     3541 gacccaaaag caatccatca ccgactctgg aattggggaa gtatggagat aattcatcga
     3601 caagtacaaa ggtgttgtgg tagaattggg gttaagcgtt gtagcaggaa ctaaatgagg
     3661 attagagatg aagaagtcaa tagcaattat gttactggta gcaacctccg ttgtggcaat
     3721 cgccgtgggc ataggaatct ggtatactcg tccctccgct cctcttgcca tcatcgagga
     3781 agcaaaagct cggcaaaggc agccactctt ggaagtaact cctcctcagc gcgccgaagt
     3841 agttccactg cctcctgact atcaaccgtt aattgacgaa ttgcttccgc ttctgcagga
     3901 tgaattgact cagccgaaag ccctctcgct tgagcagcag gcatatgttg accaagccgt
     3961 tgaaaatctt cgccgtgaat tgcttcagtc gattgacagt caggcgaatc gaacctatgt
     4021 cgatcagcag atcacctcct tgaagaatga gctcaccacc tcgcttggac gtttggagga
     4081 gaagcaggtc gatcgtcggg cgatcattga ttcgatcacc gccgatgatc tgcgcccggc
     4141 attcagcgtc ttccgggatg agctcttgaa ccaggttcgc tctgagcaac cgcgaacaac
     4201 cgtcgtgatg accggtgagg ttgacgaaga gcttctgttt aagaatctcg cttgggagat
     4261 agaaaacaat cttgacttct atgcttcata cgctcgcgat gcgattcttt ccacgattga
     4321 cgatgatcat atgatcgctc tctatgagag tcatcgtccc atgcttcttg cagacttggc
     4381 ggcgagtctg ccggctccca tgaccgtgat tgccgctgaa ccgattaccg gcgagcaggt
     4441 gctcagcgag ctggctaggg agattgagaa caacaaggag ttttacgcaa cctacgcccg
     4501 tgacgcaatc ctcgatacga tcagtgagga tgatctcttc tcttactata accactaccg
     4561 tccgctcctc cttggggatc tttatcaggg aatcaaggat gaagcgaaag tcgaaatcgc
     4621 cgattatgcc gatatcatcg agcagatgga ggccaaccgc gagtactacc ttgctcagga
     4681 ccggaaggcg ctacccggcg tcctaaccga ggcggatatc ctgccgctgt atgccaccta
     4741 ccgtgatgat cttgccgagg atctctttac ccttctcggc gaggannnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnatgcc acctaccgtg atgatcttgc cgaggatctc
     4921 tttacccttc tcggcgagga aattgagagc aacaagggaa catatgccga ctatgtccgc
     4981 tcagcgatcg gcgagggggt cagtgaagag gaggtcctcg atatccttgg gcgggagatt
     5041 gaaaccaata aggacgccta tgccgtccgg gctcgggatg cgattctgga gacgatcagc
     5101 gatgatgacc tcatcgctta ctatatgcag tatcggccgg cattggtggc ggacttggcg
     5161 gaagagctgc ctgcagctcg agccgctctt gaccgggagc aaatcttgga tgatctgtat
     5221 gccgagattg aaaccaataa accgctttat gctgcctatg tccgagacgc cttgcttgaa
     5281 acgatgatcg gtgatcccga gttcgatcag gtgtatgaag aaaaccgggt tgatattgat
     5341 gccgtcctcc tggcccttgg cgaagagatc gagaacaaca agaagtttta tgcaacgtat
     5401 gtccgaaatg ccctccttga tacgatcagc ggggaagagc ttgtcaacct ctatctgaac
     5461 taccgcgatg cattgatcga agtgggcgtc gagctggatg cgaatcagat catgggaatc
     5521 cttaccaacg agatcgaaag caaccgggaa gcatacgcaa catgggctcg aaaagcgatc
     5581 attgacacga tgagtgacga ggagctgatc tcctactacc ttcactatcg tcctgaactg
     5641 cttgaggata tctaccaagg gctgaaagat cgctttgcca tggcatcttc ggcgcccgtc
     5701 ccgtcaagcc cgttgccggc gacccagcgg acggtcatcc tccaagaggc agtggaagaa
     5761 gaggctgcac ctccggtggt cgtcccgcca agcccgtccc cggcggtcca gcggacggtc
     5821 atcctccaag aggtagtgga agaagaggct gcaccttcgg tggtcgtcga ggagaagccg
     5881 gcgccccccg ctccccgggt gcccgtctcg ccaagcgtta cgaccaagcc gacaaccgtt
     5941 ccctatgaga tgacccccgc cgaatatgag acgcttcggc aggagaaacg gaatgaagcg
     6001 cttgacgaga tattgaaaaa gatcggctaa tccgccggat aggaacgatg tccgccatat
     6061 ggagtcctga aaatcagggc tccatctttc ttttcctcaa cgtaggagtt taccagcgga
     6121 acttttccac cgccttcggg aagatcgagt gcgtagagcg gcatcgccaa gccggaaagg
     6181 cgttttcgaa gctccgcctc caacttcatt ccatcttcga tcgaaaccct cagatgagcg
     6241 gttccgctga ccagatctcc ttgaaagagg taatagggtt tgatccggtt cgctaaaagg
     6301 gtattgcaga gctccttgag ggtatcggca tcatcattga cccctttcag caacacggtt
     6361 tgattcattg ccggaattcc gttgtcgatg aaaagagcgg tcgcccggag cgcctccttc
     6421 gtcagttccc gaacatggtt gaactgggtc atcagaaaga acggggcggt atcaaacgtt
     6481 ttgagcatgg cgatcaacgc cggggtgatc cgttctgctc tggcggcagg agcccgagaa
     6541 cagaggcgaa taatcagatc gggtcgtctt gaccgaaacg ctccgatcat cgtttccaat
     6601 tggacgtcat tgagggtgaa gagatcccct ccggtgaaaa ggagttcggt gatttcagga
     6661 tgcttttcaa catagcgagc cgcttgttca atctcctctt cgtttgcagg accttggaat
     6721 gttccggtga agcgcctgcg gaagcagtgg cggcagtgga taaaacaaag gtcggtggtg
     6781 aggaacgccg cccgattggg atagcgatgg atcagccggg cggtggctgc gtgccgtttt
     6841 tccgccaagg gatcaagggt ttcccctcgg gattcggtat gctcgaagac ggtcggaacg
     6901 acctgacggc gaatcggatc ggaggggtca tcgggattga tcagtttaaa aaaggaggag
     6961 gggattttta tgggcaggag ggtgccgtcg ctttcgtcaa agcctttttc ttcttcgctt
     7021 aacgtgagct ttgaagccaa ggcggacagg gatgttatag aagcgtctcg attcatgtct
     7081 atggtgtact tgaaagaatc gctttggtct aggggtgacc ggtcgcctac atttgccgat
     7141 ccatgataca atgggcgcaa tgaggagggc gttatggcca aacaaaagaa gggtttgaag
     7201 ggatattacc tcacgacgtt tttgatcgga ctcgggtttt tcacgatggg gttgatggat
     7261 cccctctatg atacctatgt tcccatcttt ctttcccgct tcgtggaatc caagacgctc
     7321 atcggagcga tcatgacgtt cgacaacatt ctggcgatct ttctgattcc gattttcagt
     7381 gctctcagcg accgggtgcg caccccgatc gggcgaagga tgccgttcat cgtcgtctgt
     7441 ctgccggtca gcgcggtggc gttcggcctg attccgttca gcgccctttc cagcctctgg
     7501 ttcctgatca tcctgctctt cattctcaac ctcttcaagc aggcggttcg gggaccggtc
     7561 gtcgcgctga tgcccgacat gatcccggcg gatctgagaa gtgaagcaaa cggcgtgatc
     7621 aatacgatgg gcgggatcgc cgcgatcgtg ggaacggtcg gcctggcccg cctgatggat
     7681 gtgcccatca accttcccgg acgcgggccg acgaaggata tcctcgcgtt cccgatctcc
     7741 ggaatcttgg tgatcatcgc gacccttctc ctcttcttct tcgtgaagga gaagaatgcg
     7801 gtcgcccacg atgagagcga gaggcgggag ccgattcttt caagcctgaa agcggtcttc
     7861 gccgaagagg acaagagcgc ccgggcgatc ctgttcagcc tgctcttctg gttcatcggg
     7921 taccagggaa tcttgccgtt tgtcggctta tactccacct cgatccttga gacctcatcg
     7981 ggaactgcgt cgctcgcagc gggaatggtc ggcatcgctt atgcgatctt tgcgatcccc
     8041 tcgggaatcg tcgcccacaa gatcggtcga aagaaaacga tccgcttcgc ccttggggtt
     8101 ctgatcgtcc tgctgctcct catcttcttc catgatccgc ttacatccgc catggggaaa
     8161 gcgttccgcc aatatacgtt ctgggcgctg ctcttcctct ttggaatctt ctgggtttcg
     8221 atcgtgacga actccttccc gatgctttgg cagatgtcca cctaccggac ggtaggaatc
     8281 tacaccgggc tctactattt cttcagccag ctcgcctcga tcatcagtcc gccgttgacc
     8341 ggcgcctgta tcgacatctt cgggtttcga tcgatcttcg ttttcgggtc cgctgcgatg
     8401 ggaatcgcat tcttcatcat gcgaaacgtc caaggcggcg aaccggagga tcggaagtca
     8461 gaagagatga gtaaatagga tattccatcc caatatgatc aggagcacgc cgccgagccg
     8521 ctctgcatgc tcggaaaggc gtgttcccat tgttttcccc agatagagcg caagcagaca
     8581 cagggtgaaa gtgatgactc cgatcatcga catggcttga acgaggttga tcttcaggat
     8641 ggcgaacgtg atcccgacca cgaacgcatc gatgcttgtt gcaatcgata ggagcagaat
     8701 ttcacctttg aagggctctt tggcggaatg gcactctttc tcagcgtacg atctgtagag
     8761 ggtgattcca ccgaggcaga ccaacaagac aaatgcgatc cagtggtcga cccgttccac
     8821 gaagcttccg aagagtgaac cgagtccgaa tccaaacagg ggcatcaggg cttggaagaa
     8881 accaaagctg aaggcaatcg gaaatgcacg tgagaaggag cagcgtttca tcgaaagccc
     8941 tttacataag gaaaccgcta ccgcatccat cgacaacgag acggagacga agaggagttg
     9001 gacagtgttc atacgggcca gtatagggat gattgcatcc cctatcaagg agaagggtaa
     9061 aaattcaggt tcgataaagg gaagagaact ccgcggacga tcgaaagcta ctctctcgat
     9121 tccacgatgt ttttatcgcc aaaatactga atcaggatca agaccagcgc gctgccggca
     9181 aggattccat cggcgattcc ggccgggagc atgaacatca tcaggatcat cgtggtgaca
     9241 cagttgatca ccgacagggc taacccccac atcctgtttt tcagcaatcc gatcgctccg
     9301 atgagtctca acaccccata gatcaccccc atgatgacca tcatggcgag attgtcctga
     9361 aagtattgga gcttgaatga aaaatacttc cccgtttcga tcccggttct gccggtggtc
     9421 gacggaagga ggaacatgaa cagcccggcg agctccataa gcccgccgtg gatgatcatc
     9481 aagatcgctg ccgctctgta ttttctcatg gtgcgggaat cagtcgttga cccgttcgag
     9541 gtactcgcgg gtttcggtgt tcacccgaat cttttcaccc tgcttgatga agatcgggac
     9601 tcggacgctc aggccggtct ccgtggttac gtacttggtc gccccttgga ccgtatcgcc
     9661 tttcaccgct tcctccgctt cggcgacgag gtagacgact ttcgccggga tgatgatatc
     9721 aagcagctcc gcttcccaga acgtacagcg atagacttct ccatcctgca tcagaagttc
     9781 atactccgga aaggagctca tcgggacttc aagttgctcg aacgtatcgg tgagcatgag
     9841 gtggaagtgt tctccgtcgt tgtacaggta ttggcagtcg cggtcggcaa ccgtgatgtc
     9901 ttcgacgttg tcctgggttt tcattgtttc ttggagaacc tgtccggtag ccgggctttt
     9961 cagcttcacc cgaacgaacg ccgaaccctt tccggggttt acgaattcac gctcgactac
    10021 gacaaagggt tggcctttga tcagcaatgc ggtccctttt tcaatttgac ctgctttaat
    10081 catatgtatt cctcatcatg gtaaagattc ctgttgccgg cgctcaggat agcagaaagg
    10141 ggaaaaagaa tcaccccctc cagcgaagag cggcccatca tccccatcag caggcggtcg
    10201 agccccaggg cgacgccgct gacctttccg atccgggcga acgtcttggc aagcgtcggg
    10261 tcaacatcgg cgatgacgcc gccgctgatc tccctggttt gaatcagacg ggcatactca
    10321 tcccggtaat aggcttcgat cttttgttca tccgactcct cataataaca gttggccagc
    10381 tccatcccca ttccgtacag ctcccaacgc cttcgatagg ctccgtttac ctgagccaga
    10441 cactcgatcc ggcgaggata gtcgtacagc acgaggggcc gctcctgagg gaggttcggt
    10501 tcgacgaacg tcaggaagat ccgattgaac gtctcttccc agctttccgg ctcatcgggg
    10561 acggagagcc cgagacgaag ggcttcctgt ctgaggagag gttggcgctg ggtcttctca
    10621 aggtcgaagc cggcgtatcg tttcgtcgct tcggcgacgc tcatcctcct gaaggggggc
    10681 tgaagtctct ttggaacttc caatctacga aagagctctt ccgtcaaaag aatcgaatcc
    10741 tcttcatcga agccgaccga gtaatactcc agcatggaaa attcgatatt atggatcctt
    10801 cccaattgct cgctgtttct gaaacagtgg ctgatctggt agatcgaccc gaagcccatc
    10861 gcaagcaatt gcttcatgta gatctccggc gagggaagga gatagagatc ccgtgaagga
    10921 agaaaggggt tcctgaaccg ggttgcgaag ttctcgatcg tggattcggg aataatggtc
    10981 ggagagagga tcggggtatc cacttcaagg taatcccgtt catggaaaaa ggagcggatt
    11041 gcgtgatagg tccgggatcg aaagagggcg gcttcctgca tgcaaacatt gtaccgatct
    11101 gagctcttct gttgcaagct cccggtgcaa aaggatatac tcggcgcatg agtcgatatc
    11161 gaaccgatga catcatttat gctctcgcca ccccgtgggg aagcgccgcc ctagcgatca
    11221 ttcgcatcag cggcagcggt tgtcacgccc tgctggctcc atatttcagt aagagcgaag
    11281 ccctccgttc ggcaaaaaac gctacgttgt tgcatggcta tctttgcgac caggagggca
    11341 gacggattga tgaggtcgtt ctggcggtga atacaaaggg ccatggatac accctcgagg
    11401 aagcggtcga gatcacctgc cacgggtcgc tcagcgtggt tgccgaactc cttgattttt
    11461 tgtctttgat cggaatgcgg agcgccgaac ccggagagtt caccttccgc gcctttcttt
    11521 cagggaggat ggacctcact caggccgagg cggtccatga actggtcgag gcgcaaagcc
    11581 cggttgcccg cgctcaggcg ctcgcccgct tggacggcgg attgaaagaa aagctcaacg
    11641 ggatgaagaa tgttctcttg gccttgctcg caccagtgga agtgcagctc gactacagtg
    11701 aagatgagct cgacgagttc gtctttcccg atgagctgct tgattcattg ctcaatgacc
    11761 ttggtcgtct cttgcagagc tatgcgatcg gaaccctcta caagagcgga gcccggatcg
    11821 ttctggccgg ggcgacgaac gtcgggaaaa gctccctgtt caatctcctg ctcaaggaag
    11881 agcgctcgat cgtcagtccg gtccgaggca cgacgcgcga ttacattgaa gcggatctcc
    11941 tcttggaggg aattccgatc cggctctacg acactgccgg cctcagggag agcggcgatg
    12001 agatcgaacg tgaagggatt cgccgaagcg agcacctgat cgggcaggcg gatctggtga
    12061 tccatctcct tgattcgacg gaggagggtg tggatcgggc ctttgatggg cgaacgatcg
    12121 tggtgtacaa caaagcggac ttgcgccgtc cccctgaggg agcgctggcg atcagcgccg
    12181 ctacgggtga gggaatcggc gatctgcttt ccgaagtcgc aaggcggctc aagcgcggag
    12241 tcgaaggagt tgatgaaagc aggatcgtga tccaatcaaa gcggcaggcg gatgctcttt
    12301 tggccgccga agccgccctg aagggggtga aggaggatct tcaggccgca cttcccttgg
    12361 atatcgtggc ggtcggcctg aacgaggcgc tgtaccactt gggcctcatc accggagagg
    12421 tgactcccaa tgatattctg gagtcgatct tctccaattt ctgtgtaggt aaataagatg
    12481 gattatcatg cattggtcgt aggcggcggg cacgccggta tcgaagccgg cttggctctc
    12541 agccgaatcg ggttcaagac tctcctgata acgcagaatc tcgatgcgat tgggcgcctc
    12601 agttgcaacc cggcgatcgg ggggctttcg aaaggaaaca tcgtccgcga ggtggacgcc
    12661 ctcggcggtg aaatggcgca tctgatcgat cgttcgatga tccagttccg gatcctgaac
    12721 cgcaagcgcg gtccggcggt tcaagccccg cgggctcagg cggacaagta cacctacgcc
    12781 cgcctcgcca aggagaccct tgaagggcag gcgaacctcg agatcttcat ggacaccgtc
    12841 gtcgatctga tcatcgaacg aaacaggatc gtcggggtga agaccaagcg cggccatgaa
    12901 atctccgccg aggtggtggt tctcaccacc gggacgttca tggaaggacg gatcttcatc
    12961 ggcgagtatg atgcccccga gggacggctg gaagagccgg cggcgatcgg gctcggaacc
    13021 gcaatgcgaa aaaaagggtt cccggtggga cgtatgaaaa cgggaacccc cgcccgggtt
    13081 cgcaagtcct cggtcgatct cgaccggctt gaggttcagg agggggatga ggagtcgatg
    13141 agcttcagct tcgattacga cggtgtggat cgaccatccg ttccctgcca catcacgtgg
    13201 acgaatgaga aaacccaccg gatcatcgcg gataacatcc accgctctcc gctgtatggg
    13261 ggaaagatcg tggggcaggg cccccggtac tgtccttcga tcgaagacaa ggtggttcgc
    13321 tttcccgacc gggatcgtca tcagattttc attgagccgg aaggactcgg aaccgaggag
    13381 ctgtacctta acggcatctc ctcatccctg ccggaggatg tccagagtgc gttcattcat
    13441 acgatcgaag ggttggagaa agctcaaatc atgcgccccg gctacgcggt cgagtacgat
    13501 tacatcgacc ctcaggccct ctttccctcc ctggagagca agcttgttga aaatcttttc
    13561 atcgccgggc aaaccaacgg gacgagcggg tatgaggaag cggcgtgcca gggcctgatc
    13621 gccggaatca atgccgctca gaagctcaag ggggagaagc cgctgatctt ggggcggaat
    13681 gaagcgtata ccggcgtgtt gatcgatgat ctcgttactc tcggaacgga ggagccctat
    13741 cggatgttca ccagcagggc ggagtatcgc ctcaatctcc gccatgacaa cgccgaccag
    13801 cgccttaccc aaaaagggta tgacgtgggg ctccggacgg aggaacatct acagcggctt
    13861 cgagagaagg tgagtcggat cgagagcgtg aaggatctgc ttcgaataca ccgctatcgg
    13921 gagaagagcg cgtttgatgc tctcaagatg ccggaggtca cgatcgaaga gctagccaag
    13981 tcgattcccg aactgggcgc ctttcctcca tcgatccgat atcaggtcga gcttgatgtg
    14041 aagtatgacg ggtatatcaa gcgccaagag cggcagatta accgcttcga gcgctttgag
    14101 acgatgacga ttcctccgga tctggattat gatacgctgg aagggctgtc gacggaagca
    14161 cggcagaagc tgaagcatat tcgtccgttg agcgtcggac aggcgacccg aatcagcgga
    14221 atgcgctcca gcgatgccgc catcctcatc attcacctcc aaaggcggtc aggcgatgcg
    14281 tagatacgat cgtctgcttg atgaaggtct tgctcgatta gatctctttt ttgaccctga
    14341 gcagcggtca cggctggata catatatcgc tgaacttgag ttgttcaacc cggtgtacaa
    14401 gttggtgggg gcgcaaggag aagagctggt gatcaaccat attttcgatt cattggcggg
    14461 agtcaagacg attgaagaac ttgcaaaaag atatgatcac cctacgctgg cggacttggg
    14521 atcgggagct ggcctgccgg gcattcccct tgcgatcgcg ctaccgaatc tggatgtcgt
    14581 gttggtcgaa cggatgggtc gccgagttga ttttcttcga accgcattgg tagtcacatc
    14641 cttgcaccgg cgtgttacaa ttatcgatcg ggatatcaag gagttgaacg aacgctttga
    14701 agttcttacc tttcgggcat ttcggccttt ggttgaaatt cttgattcag ttgcgcctct
    14761 tctccaggtg ggtggggtgg tggtggcgta caaaggcttg agggcgaacg ttgaagaaga
    14821 actgaaagag gtaaagcgga aggtcacttc ccgatgggag gaggagttga tcgatctgga
    14881 tgttccgttt ctggaacgga gtcgaatgct ttgcctcttg cacaagattt gataggatgg
    14941 aagggtatga aaaagaaagc gggtgtaaaa aaaacaaggg gagcgatcat cgctgcgctg
    15001 gcggccttca tctgggccgg tatccttgtg cttccgagct tcggcattga gcttccattc
    15061 atggaaatga tcttcttcct cttctccttc tcgacggggg acaatcctct ttggcttttg
    15121 ccctcagctc tgtttttcct tgttgcaagc atcatcctca ttgtgaatcg gaaacgtccg
    15181 atcgtgcttg cgttccaact gttctattcc gcccttgtcg cttatacgct ccatagcctg
    15241 agtcacattc ttcaggatag tccatggccg atcttccgga cccatctgct tgagcgtacg
    15301 gagggcgttc gaagcgcctc atttactctg gtggtgttct tcatcggcga gatcctgctt
    15361 ctcatcatca tcgtgctgat cagtgatgcg atcaggcatc gaaggcgtcc ccgtttggaa
    15421 gaggaacggg tcgacgagcc tgaggagacg gaaaccgtta cgttcgtgcc caaggcgagg
    15481 gccgagttcc cgaatgttga cgatcttccc gagtatgtgg accgtactcc gccgcctccg
    15541 aaacccaaac gggagaaagc accggcggca gaggagatca gcggggagaa cacggtgagt
    15601 ctggcgatgt tccgtcgtct ttccgaagag gtgaagaagc gggaagagca acagaacaaa
    15661 ccgtctatcc cgtcaccggc caaggggaag agcaggagcc gggtcgatga gaagcagggg
    15721 gccaaagagc ttccaggctt gatgctcgcc gccgtgaaac ggatggaaga acggaaagag
    15781 gaaaaagtcg tcgaggaaaa gccccgttcc tttttcgccc gccacagcga accctcgacc
    15841 ttggagatcg aagaaaaagt tccttcacca cccccgcctc ctcaaatgcc tcaaccggaa
    15901 gaaccggcgt ttgcggctcc tcccgtcgtg gagcattcgg aaaaccgggt cagcgtcaag
    15961 attcctcctc aggtcgagca accggaactc ttcgatgagg atgatccctt ggagagcagc
    16021 aggggaatag gaggcttgga tgcttcagcg ctcatctcca agcccaagat cggatatcgc
    16081 tttcccagtg agtcgatcct ccgtacctat gagaagatcg gggatgtgat tgatgaacac
    16141 acactccgtc aagggcagtt gcttgttgaa accctccttc agttcaatat ccaggtagat
    16201 gtaaccaaga tcgtccgcgg tccgaccgta acgatgttcg aagtgcttcc cgctccggga
    16261 attcgagtcg gatcgatcgt gagtctcagt gacaatatcg ccttggcgct cgctgctacg
    16321 caactgagaa tcgtcgcccc gattcccggc aagagcgccg tcggaattga aattcccaac
    16381 aagaagcggg atatcatcgg ctttaaagag atgctcggca gcgtcacgga gaagatgaag
    16441 attccgatga ttctcgggcg aaccattctg gatgaaaagc gggtgatcga tctgagttta
    16501 tcaccccatc tgttgattgc cggatcgacg ggaagcggga agagtgtgtg catcaacagt
    16561 ctgatctgca ccatcctcta tagacgaact cccaaagagg tgcggatgat cttagtcgat
    16621 cccaagatcg tcgagctcaa catctacaac ggaattcctc atttgctcac tccggtcatt
    16681 tccgatgcga agcggacgct caaggcgttt gatttctgtc tcttcgagat ggagcggcgg
    16741 tacaatctgc tccgggcgct gaatgtccgg aacatcgtcg gctacaatga gaagatcgca
    16801 gcgaacaacc tggcccggga aaagcttccc tacatcatgg tcgtcatcga tgagttcgcc
    16861 gacttgatgc ataccgtcgg caaggagttg gagaccaagg tggcccgcct cgctgcgatg
    16921 agccgagcgg tgggaatcca tatggttctt gccacccagc gccccagcgc cgatgtgatc
    16981 accggggtca tcaaggcgaa cataccttct cggatctcgt ttgccgtaac cagcttgatc
    17041 gacagccgga tcatcctcga cgagatggga gcggagaagc tcctcgggcg gggggacatg
    17101 cttttgcaaa cctccgattc ccccacgatg gagcggatcc agggggcgtt cttaagcgat
    17161 gcggaggtgg aggagatcgt cacctttgca accggccaag gggagccgga gttcatcgat
    17221 gagtcgttct tcgaggacga tcctcccccg atcaccgaga gcaccgtcgg gctcagtgat
    17281 gcgatcaccg atgaggagct gatggaacaa gcacttcaaa tcgtggtgga acggaaaggt
    17341 gccagcgcct ccttcctgca aaggcggttg aagatcggat acaaccgggc ggcccgcctc
    17401 gtcgagcaga tggaagaaga agggtgggtc ggaccggcga acggttcaaa gccgcgtgag
    17461 ctgttgagga tcccctaaat gtacgcatcg agaaacgggg tgattatccc caaggaagag
    17521 gcggtcgtgc cgatcacctc ccgcgaagtc cagagcgggt tttacgtgta tgagtctttg
    17581 cgggttctgg gcggattcgt cacccgcatc aacgaccacc ttgatcgatt gcttgcctcc
    17641 gcaaaaggaa ttcatttgac ccatcccttt acgaaggaat tgatccgcct ctgggtggac
    17701 gagctcatca aggcggaaca cattggcgat gcgaccgttc gaatcaccct ctacggcggg
    17761 gcggatccgg ttctcttcat cgttccggtt gaacttttgc gctatcccga ttcctattac
    17821 cgggatggag tgaaggcggt cacctttgaa ggagagcggt tgtttcccac ccataagagc
    17881 gggggtctgc tgctcaacta tgttgccctt gaagaagcga agcggttcgg gggattcgag
    17941 gcgttgctgg tcgatcggaa cgggcgggtg ctggaaggaa cgcgcagcaa cttcttcgca
    18001 ttctcgaaca atacgcttca tacggcggat gaggatctcg tccttggcgg aatcacccga
    18061 agcgaggtgc tacgctccgc ccgggagctc gggttcacga tcgatctgaa tcctatctat
    18121 ctggaagatc tgtttgccgg ccgctttacc gaagtgttca tttcagcgac cagcatggcg
    18181 gcgatgccgc ttcacacgat cgatgacctt cgtttttccg ctccctttga acggacccgg
    18241 gcgctccacg attgcgtgcg cgcccgtcag tggcaatagc gggaattggt ggacagagct
    18301 tctcttttac tttatcgttg aaggaggcgc cggcgcctgt cttgttgtga ttatgttact
    18361 tcttataaag gtgaatgata taccctatga atttttctga attacccctc catgagcagg
    18421 tcctcaccgg tattgaacgg accggctttg aacagtgcac cgatgtccag cagcgggtgc
    18481 ttccggtaac acttgccgga aaggacgtga tggttcaatc gaaaaccggc agcggaaaga
    18541 cagccatcta cgccattacg attctccaaa gctttattac cgcccaagcc gacaacagag
    18601 aggtcaaagc cctcatcatc gctccgaccc gggagctggc ggtgcaaatc aaggcggata
    18661 tcgatgaatt ggccggcggc gtgaataact ttcgagtcgg ctgttttttc ggtggcgtcg
    18721 gctatgccaa gcaggatgaa tggatcgaga agggccttga cgtctatgtg gcgacacccg
    18781 ggcgcatctt ggattatcaa aagatgaaaa aggtcgattt tcgtcaattc gatatcttcg
    18841 tcgtggatga agcggaccgc ctcttcgata tgggcttcta tccggacatc caaaaaatgt
    18901 tcagcttgct ccggccggtg aaagaacgcc agacgatgct tttcagtgcg acgctgtcca
    18961 caaaggttcg tcaccttgca tggcagttca tgaacgagcc gaaagagatc gaggttcaac
    19021 ccgaggagat caccgtcaag gagatcaccc aggagctgta ccacatttcg aaagatgaaa
    19081 agttccgcct cctgctccag ctgctgaagc aggatgagcc cgtctcctgc ctgattttca
    19141 ccaacacgaa agctcgctgt gtcgaagtct ccaagcgact gagcatgaac ggatatcaga
    19201 gcaaatacct gatgggcgat ctgccgcagc gaaagcgcct tgagatcatc gggtggatga
    19261 agagcggaaa gctccgcttc ctcgtcgcaa cggatgtcgc tgcgcggggt cttcagatcg
    19321 acgacctcgc tctggtggtc aactacgaca tccccgaaga ttacgaaagc tatgtccacc
    19381 ggatcggccg gaccgcccga gccggaaaga gcggcaaatc gatcactctc gccgatgaag
    19441 agtacgtctt caaccttgag gcgatcgagg agttcatcca gatgaagatt ccggtcatct
    19501 ggccggagga tcttcccgag gtcgaggaca agagcgcatc ctacacgttc cgcgatctgg
    19561 ttccgcggac cggagtccga tcctcatcgg cgcgccgtgg gggcacccct tcaccgagcc
    19621 ggggcaggaa acaggcgccc aggcgcgccc cggaacggaa acaacgccgc ccgaggccgg
    19681 ccgcaccgcc gaagcagcat ccgccgcgcc gcagcgccaa agcatacgcc gacctttccg
    19741 cccttccgct ggaagagcgg cttgcctact acaaaaagga atatcttggg aatcaacaac
    19801 cggcggatga gccggtaaag aaaacttctt caccaaaacc ggttgatgcc aaagaggaac
    19861 aagcgaagaa gaaaggattc tttgcccgat tgtttgcgaa ccgggggaaa tgatgctgaa
    19921 gcgttttgtc tcgtactatc gcccctacaa gggtttgttc ttgttggatc tggggatggc
    19981 ggtgctgtct tccatccttg ccattctgtt ccctgcgctg acccggcacc tcttgagcgt
    20041 tgaaataccc gctggaaatc ttcgggcgat ggtggtgatc tttgtcctca tgatcgcaat
    20101 ctatctgatc cagaccgtca gtcaatatat ccggatcact tgggggcata tcctcggggt
    20161 gaagatggaa acggatatgc gcaatgagct gtttgcccac cttcagcggc tctcgttcgg
    20221 gtatttcgat cggaccaaga ccggtcacat gatgagccgg atcaccaacg atttgttcat
    20281 tatcgccgag gtggcgcacc atggccctga ggattttctg atcagtttgg cgacgatcgt
    20341 cggctcctat gtcctgatgt tcatctacag tccaccgctt gcgttgatca gtctgattcc
    20401 cttcccgttc atggtgtttt acgggtcgta tttcggacgc aagatgcggg cgacgttccg
    20461 cagggtccgc tcgaccgtcg ccgatgtgaa cagcaacgtg gagaactcgc tgatggggat
    20521 tcgggaagtc aaatcgttcg gccgggaaac ctttcagacg gagaagttcc aagaggtgaa
    20581 caacattctc aaagaatcaa agatgcagca gtacaaggtg atgggctcct accacgccct
    20641 gatggggctt ttcagcaacc tttattactt ctttaccgta gtcggcgggg cgattctcat
    20701 catgagaggc acgatcgagt actacgatct gatcaccttc attctctatg tgggcatcgt
    20761 cctacccccg atcgaccgct tgatcaactt caccgaacag cttcagcagg ggatggcgag
    20821 ctttgaacgc tttgtccaga tcatggacga gcatccggct atccaggatg ccaaggatgc
    20881 cagagagctg acggtgaagg atggaacgat cgtctatgag aacgtctcgt tcacctatga
    20941 gaataccgat acggcggtct taagcgatgt caacatgacg attccaggtg gagagaccgt
    21001 tgcgatcgtc ggagaatcgg gagcgggaaa atccacgttc gcttcacttc ttccccgatt
    21061 ctatgaaccg caagcaggtc ggattctgat cgacggcacc gatatcacga ccgtgaggca
    21121 atcatcgctc cgcaaggaga tcggctttgt ccagcagaac gtctttcttt tcgatgacac
    21181 gatcagggag aatctccggt atggaaaggt cgatgccact gacgaacagc tttgggcggc
    21241 gctcgatgcg gcgaacctcg gacagttcgt tcgctccctc cccttgggtc ttgaaacgca
    21301 ggtaggggag cgggggacgc tgctcagcgg cggacagaag cagcgggtct ccatcgcccg
    21361 ggtgttcttg aagaatccgt cgatcctgat cttcgatgag gcgaccagca gcctggatac
    21421 ggagagcgag gagctgatca ccgaagcgtt tgaacgcctc agcgagggtc gaacagcgat
    21481 cgtcatcgcc caccgtcttt cgacgatcaa gaatgcggat cggattctgg tgcttgatca
    21541 ggggcggatc gtcgagtcgg gaacccacca gaacctgttg cagaaaaacg gccagtacgc
    21601 caaactttat aaaaaccaag actttatgta aggagcatcg gttccgtgaa agcgcttcgc
    21661 attacgtttg ctgtcatatt tgttgttcca attctggtcc tcagtcctct ctatgcgttt
    21721 ttcattgcgt atctcatgga agtgaccgga atggagaaag cggcacagaa ccaccttcgg
    21781 cgctgcggct ccttcatcgg ttcggcgatc ctcttcttcc tcggggtgaa gctggttgtg
    21841 acgggaaggg agaatctgcc ggaaacgcgg gaagggatct gctacatggg gaaccaccag
    21901 agcatgctgg atatccccgc ctttgtggga ccggccaagc tgtgggggtc ggtgatggcg
    21961 aaagtggaag tcaagaagat tccgatcatc aacctctggt gcaaggcgat gaagatcatc
    22021 ttcatcgatc gcaaaagccc tcacgattcg atcaaggcga tctttacggg agtcgaacat
    22081 ttgaagagcg gtggaaacct tctgatcttc cccgagggaa cccgttcgaa aaccggaaaa
    22141 atcggggaat tgaaagccgg ctcgatcaag ctggcgaccc gcagccgggc tacgatcgtt
    22201 ccgttcgcga tcaagaacct ccggcccggg tttgaaggga tcaccaatct caagcgagtc
    22261 cacgcttcca tggctatcgg aaagccgatc gagacgaagg atctgagcaa agaggagctt
    22321 gcaggtctgc atgaggtagt ctatggagcc atcaccgatc tcttcgatca actcccttga
    22381 tgtaacgaat ctctcctttt cctatcaagg atgggtgggc gagaatccac caccactctt
    22441 tgagaagctg tccttttcca ttctcagagg aagcaaagcc ctcttgcttg ctccctttga
    22501 cagcggcaaa tccacccttg ggcggatgct tgtcggagga atcccgaaat acttccccgg
    22561 gaggttggaa ggcgccatca aggtcatggg gcataacctt gccgatatcg aagtctggaa
    22621 tcttctggat gtagtgacgc tcgtttctca aaacccgcag gagcagttcg tcgcttcgac
    22681 cgtagaagaa gaggtcgcct ttggattgga atcattgggt cttccgccga aagagatgag
    22741 aagacgggtg gaggacgctc tgacaaattg gggtctcgat gaattgcgac aggtttccaa
    22801 cagcgaactt tccggagggg agcgcaagcg ggtgctcctc gccgcagccg aggcgcttga
    22861 tgctcctttt tggattctgg atgaagcgtt cgacgatctc gaccagcggt ggcgggaaca
    22921 cctggcacgg ttcattcaaa ccaccgataa aacgattctc ctcttctctt cccgctatct
    22981 tgcccagttc gatcgattgt ttgatcatgt gtttctgctt gaagacaagc aggttcgcaa
    23041 ggtagatccg aaacaagctg caacggtgtt cgcgtctctt gccggagaca cgaagccaaa
    23101 ccccctttca ggacgagcca aagaatatgc ccatcaacat acgttgcgcg tttcgaatct
    23161 cgtcgtggag cgccggcgct tttccgcttc atcaacagaa acgtttcatc tgcaggtgcc
    23221 ggccttcttc ttacagagcg gagagatcgt aagcttggtc ggtccgaacg gaagcgggaa
    23281 aagctcgctc agccgagtcc tgtgcggtct tgacgatccg ttggagggaa cgaggataat
    23341 cgatggcgag gtgctggatg gttcgatgct gaatcgaagc gtcgggtatc tttttcaaaa
    23401 ccccgacctc cagatcttcc ttcccaccgt caaggaggag ctttcctatt cattgaggcg
    23461 ggtcaagggg ctcagtgatg aagatatttc caagagagtg gctgaatgcg ccgatctttt
    23521 tgaattgaag ctttcccaca ccccgtcgac gatgagctat ccgaagcgga aagcccttca
    23581 ggcggcggtg tattacctgc ttgaccggcc tttctatatt cttgacgaat tggataccgc
    23641 gctgacctac cacagcgctt tgacgatgat ccgccacctg cagcgaaatg gtgcgggaat
    23701 cctcatcatc acccatgatc aacaattcat aacccgcaca ggcggaagga tatatcagat
    23761 cgaagaaggg agggtgagcg agcgatgacc gtgaacacat acctttcgaa caccggctgg
    23821 ttgtatcggt tcgatccgcg ggcaaagatc ttcttcctcg ctctgttgct tgggtgggtc
    23881 ttcctgcccg ttacgcttac cggtttgggc tgcatgctgc ttttgattct gttcaccgcg
    23941 ttgagcaatc ccggtccccg ctttgtccgt tcgaccttca cctcgattct tccgatgatc
    24001 atcttcatgg tgctctttgc tccgttcaat gtccgcagcg gaaaagggtt gatcacgatc
    24061 cgttcctttg ttctgctcac cgaagagggg gctctccagg ctttgcggct tgccatccgc
    24121 ttcatttcca tcacctatct tgcaaccctg ttctttgcca ccaccccgat gcccgagatt
    24181 ctgttggcgc ttcagtggta tcggcttccc tacaacgctt ccctgatcat caccttggcg
    24241 ttcacgtaca ttcccttcat cgccgacggg ttcgcgcaga tcgaggagtc tcatcggctt
    24301 cgggaagcgg gagcccatct cgagcaaaag cggatcggca agatccgctc cctcataccg
    24361 accctcacca gcagtctcgt cctcgccctg cgttcaattc ccacgctcgc cctcagtctt
    24421 gagctcaggg gatatggaag ggcgaacaag cgaacccgct accacgattt gagttcctat
    24481 cgccgaacct ttaccgactt tttgatttcc ggtatgattc ccgtgatctc gttccttgtc
    24541 ttcaaggcct gatcggaggc gcatgatgaa caaagaaaaa cacgcgctca aggttgcggt
    24601 ggttgcggtg ttgacagccg tggtggtggt tttcacgctg gtgatccgga ttccgaccac
    24661 gaagggatac ctgaacctct gtgacgtggc gatctgtttc atcgccttca ccttcggtcc
    24721 tgtctccgct tttctggccg ccggtctcgg cacggcgatc gccgatctta tcagcgggta
    24781 tgcccaatgg gctccgatct ccttcatcgt tcatggcatc gaaggacttc tcatcgccct
    24841 gatcgtcagg aagcagccgt tgtcgaagat gcgcacgttc ctggccgcga tcgtgtgtat
    24901 tctcaccgtc ggtttggggt atttcattct ctctgctttc ttcatttcca ccgtggcggt
    24961 cgctgcggcg gaaattcctc ccaacatgat tcaggcgggg gtaggggtgg tcttcggttt
    25021 cgctctatcg aaggcgatca agcgggcgta tccgccggtc tccgccctct catggtaaag
    25081 gagcagctgt atgtatgatc gtgtcgccgt catcgattat ggaaaaggtg atgccgtcgg
    25141 tctcgcccgt tcaatccgat cgcgcgggat atacagcagg atctttccct acaccgtttc
    25201 aaaagaacgc ttggctgaat gcaaaccgaa gcttatcatc catgcaaccg acaaggagtt
    25261 tgaaagaacg aacctcgctc cggaagtcac ctatccgtca accgacctga acgaggttct
    25321 gctttcccac gtacaagcga actggacgat ggagcggtat gccgaggatc tgattctgca
    25381 gatcagagag acggtgggca ggaagaacgt gctgctcggt ctgagcgggg gagtggattc
    25441 ctccgtgtgc gccctgctta ttcacaaagc ggtgggtgat cggctcaaat gcgtcttcgt
    25501 cgatcacggg ttgctcagaa aggatgaagg ggcggcggtg aaagcgctct tcgaagagac
    25561 gtatcacatc aacctgacat acgtgcatgc cgaagaggtg ttcctttccg gtctgaaagg
    25621 agtaaccgat cccgaagaga agcggaagat catcggcaaa cttttcgtcg atacgttcta
    25681 cgaagccggg cgaaagctgg gggatatctc ctttcttgct caaggcacga tctatcccga
    25741 tatcgtcgaa agcgtccaac cgggcagaac gcttgcagtg aagagccatc acaatgtggg
    25801 tggccttccc gaagatctca gctgggagtt gctcgagccg ctcagcgacc tcttcaagga
    25861 tgaggtgcgg gcattgggcc gggtattggg cctgcccgat gtccttctcg gccgtcatcc
    25921 cttcccggga ccggggctgg gtgtccggtg cataggggcg gttacgaaag agcggctgga
    25981 gacgcttaag gaggtcgatg cgatcttcat cgatgagatc aggaaggcga atctctacga
    26041 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaggaggt cgatgcgatc
    26161 ttcatcgatg agatcaggaa ggcgaatctc tacgatgaga tctggcaagc gctcgctacg
    26221 cttcttccgg tgaagtcggt gggagtgaag gatggaaaac gggtctacga agaggtctgc
    26281 accctccggg cggtgaactc catcgatgcg atgagtgctt caatctaccc gttcagcggt
    26341 gaattcatga acagaacagt gctccgaatc ctgaatgagg tacccacagt cagccgagtc
    26401 ctctgggatg tctctccaaa accgccggga acgatagagt gggagtagtt aaggcactat
    26461 aagaaaccgt taaaattact gattattcta aggtttcgag tggtcattca gtaccatgtt
    26521 gtttgttttc ttcatgttct cctttttacc attctaaaaa attcatgatt acattaatca
    26581 taatttcttt ttcttccggt tttgattctg ctaatagcaa agtgattgct accaatgtac
    26641 tgtcagaaat aattttttct tcatctttga acagtaaatt atttttgttt aggaaatata
    26701 ggaatatagc tgcagcaatt cttttgcatc catcaataaa aggatggtct ttgactatga
    26761 aataaagaag gtttgccgct ttttcttgag tggatggata tatatcttct ccaaatgccg
    26821 tttgataaac ggagccgatt ataccttcta atgcaccttt gcttttttct tttccaaata
    26881 tagttgatgt agtggtaaat gccatgctat caatgagatt acgacactcg ttatagctta
    26941 gttgatagct ttcttcatta gttggtttgg gcttttgaac aacttggtga tcgtaatcat
    27001 ctaataactc aagtgcttga gtatattttt gaataacatc aaaaacttct ttagaatcta
    27061 gttcaagaac atcggcaatg atgtttgatt ggatttctat taccttgttc aatactttta
    27121 atcgtttgtc attaactgca taaccttgaa caatatattc ttttaagata gatgtagccc
    27181 attttctaaa agcaatacct ctctgagatt ttactctgta gccaacggat ataatcatat
    27241 cgaggttgta gtaatcaaaa attctttcta cggatctgtt gccttcattt tgaacttgtg
    27301 caaattttgc acaagttgaa ttctgctcta attcattttc cttaaatatg ttttttatgt
    27361 gtctttgtat tacagaacga tccctgtcga aaagttctgc catttcttcg acccttaacc
    27421 aaacagttcc tttatcgggt gtcacttcta cgggaagctc caattcaccg tctttataaa
    27481 taataacctc attactcata aaatataaca cctcctaaag gcaattttaa aatggtcttt
    27541 gaagagacga cacttgcatg catatgaaga ttcaccttgc ctgacatcgt ctcttccgtt
    27601 cgggcatcaa ttcttggtga agcaggaatt ttgtaaataa ttgcttttaa tatatagaaa
    27661 aagaaccctt ttctgatata gttggtaatc ttaaaaacca ccggaaaagg agctcaattt
    27721 ctgtggccta ttctacatca aacacccttt cccttcaacc tctgctcttc aattccgaag
    27781 atttcagctc atgcttcttc aggaagccta ccgaacgtga actggagttc ctcatcctct
    27841 atagtgaagg tgtatcgtta gcggaaggcc caggcggaac tagagaggct tgaatacctt
    27901 gctgaagact tcattaagtc aatctctcaa ctggaaatgc ggtgctcact gactgcaaag
    27961 cagaactcga agggcatgaa acagtggttc atagggt
//
LOCUS       DAOSLI010000022        27743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_1922_length_27743_cov_25.560857, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000022 DAOSLI010000000
VERSION     DAOSLI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 27743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27743
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1922_length_27743_cov_25.560857"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3034)
                     /locus_tag="PLF67_04705"
     CDS             complement(<1..3034)
                     /locus_tag="PLF67_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOE89350.1"
                     /translation="MAVEPAPVVVAPPVVEEVVYPYGVRPIVKNADGNEVFDLFIVHT
                     NDVKGNIYAENGGVGIAKLSTALKAGREITDNWLLLNTGYVGEIPAEAALTAAWIVDE
                     LGYDAYLPQAIQIELGIEGTQKAIPLAANVLDAEEYLLFQPYQVYDFNGFTVGVVGIV
                     APKPVSGVSFDADVILDNAQWAVDIARTYVDYLVVLTDLGEGVFSSSDLAENIQGIDL
                     IVDGSGSETAKVVNDTLIVRAEEQLRSIGGVVVSVDRGRVTGVLPLVLPAEDLAVPYL
                     SPLAAEYEPFANALGYTLMVTLPEDPAIVEQIGPTPEKVAVVVPVPVVKVEEKPAPPP
                     APAPAPPPAPPAPAPVVEEVVPIKITRPVEPREFPLGVTSIVKNAAGDKDFDLFVVHT
                     NDVHGRITPSDGGMGYSKLATMLEVGRSITDNILVLDAGDVSHGTNLANLFEGEVVGV
                     LLDMLGYDAVVPGNHDFNYGSERLIQAAEFAEEYSNLRVLAANVLDEDGYLVFQPYQV
                     YDFNGFTVGVVGLTTPDTKTKSHPKNTAGLTILNEEIFAIGQQAIDIARTYVDYVIVL
                     GHIGLDPDGASGLTSDMIVSKIKGIDLFVDGHSHDALEKGLKIGDSYIVSAGEYLENV
                     GVVEINVRGGKVVGTTPMLIPAEDVLNPEESDLARAYGIVDVPNHLKVDEYVTYMQAQ
                     LSDKLNQVIATIPVDLNGERAHVRTRPTNLSKIITAAMTEQSGADFTITNGGGIRASL
                     KAGPVTIGDVINVLPFTNILTVVEATGAEVYAALEHGYRMLPETNGGFAQTDLSVVYN
                     RFAEPGKRIVRVLLNGKAIDKNKTYRVATNDFLAAGGDGYTMFGRILVEGDLLSDVLI
                     KYLSTKKLTAEMLGDDVKEVVVAEEKPAPAPEPAPAPVPAPVVAEVKPAKVEAVEYPL
                     GVMSVVKNSAGDKDFDLFVVHTNDVHARITPSDGGMGYSKLATMLEVGRSITDNILLL
                     DAGDVSHGTNLANLFEGETVGVLLDMLGYDAVAPGNHDF"
     gene            complement(3489..3659)
                     /locus_tag="PLF67_04710"
     CDS             complement(3489..3659)
                     /locus_tag="PLF67_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89351.1"
                     /translation="MAGNVSKNARREGTKVMTSRWGGTIKMKSVFENGKLRHMAYCEK
                     SGNTARKPKDLM"
     gene            3756..4034
                     /gene="yidD"
                     /locus_tag="PLF67_04715"
     CDS             3756..4034
                     /gene="yidD"
                     /locus_tag="PLF67_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOE89352.1"
                     /translation="MKRFLRQLFLIPVYLYKGLLSPFFGSSCIYHPSCSTYMVEAVLR
                     HGIIKGSIMGIARILRCSRWFLGGEDPVPEDWSFKAIKDEFTRHRRRS"
     gene            complement(4009..4635)
                     /gene="udk"
                     /locus_tag="PLF67_04720"
     CDS             complement(4009..4635)
                     /gene="udk"
                     /locus_tag="PLF67_04720"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOE89353.1"
                     /translation="MKEVKIIGITGGSGSGKSTIVRKISEVCSDFVFIPQDNYYKSAA
                     YISNENITAFNFDHPDAFDMDLLYRHLSDLKAYRPIKMPLYDFVNHRRRDETVHVDPR
                     PLVIIEGLMVLHDPEIRALLDLKLYVDTPDDIRFIRRLKRDISERGRTLESVCAQYLE
                     VVRPGHFNFIEPTKVYADLIIPEGGYNESALSVLIPFVKGITSADDDV"
     gene            complement(4643..5677)
                     /locus_tag="PLF67_04725"
     CDS             complement(4643..5677)
                     /locus_tag="PLF67_04725"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOE89354.1"
                     /translation="MVHVQKIAESPKGEDIFKVTLESGPYTVQILNLGATIQSLIVPD
                     RDGKKRDIVLGFENPFEYLGHSAYFGQVVGRFANRIKDATFTLNDRTYKLDANDGKNS
                     LHSGSANWGWRLWAIEPFEWEEKPGVVLSITSQAGEGGFPGSVTATVSYLLDGESGQL
                     RLEYEATASEETPMNLTNHSYFNLAGAGSGTILHHDVQLFCDRYVEVDDHAIPTGKVL
                     GVEQTPFDFRKSKAVGLDIETAGGYDHCFILSDERSELRPAATVYDPGSGRMMEIFTT
                     LPSLQFYTSNFLDGSTVGKGGVPYQKHAGMCFETQYYPDSPNQAHFPNAIFGPDRPYR
                     HTTVFAFSTK"
     gene            complement(5693..7510)
                     /gene="lepA"
                     /locus_tag="PLF67_04730"
     CDS             complement(5693..7510)
                     /gene="lepA"
                     /locus_tag="PLF67_04730"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOE89355.1"
                     /translation="MTLDSSLKRNFCIIAHIDHGKSTLADRFIERAQIRQNRGPEQTQ
                     ILDNMDIERERGITIKSQAVMIPYKAQDGKTYELNLVDTPGHVDFSYEVSRAISSCEG
                     ALLLIDAAQGVEAQTLANLYMAMEHNLDIIPVINKVDLPSADIEECLHQIDHDLGLDP
                     DMAVQVSAKTGFGLGELFEAIVNHISPPSGDPKASLEALIFDSHYDPYRGVVVHCRIF
                     NGELKDGDEVTFMHTDTTYRAEEVGLFQLGLQKRDKLSAGDVGYVITGVKTISDVRVG
                     DTITSAAKPAKRPLPGFKDVKPVVFSSIYPVDTNDYEELVDALDRLKLNDASLVYEKD
                     SSAALGFGFRCGFLGMLHLEVVQERIEREFGLSIVFTSPSVQYHVHMKDGSMLSIDNP
                     LEYPEPMKIDYAEEPYIQANIITPVSYLGPIINLCIDRRGTQVGMNYLDAKRVELIYE
                     MPLAEVLFEFYDRLKSVSRGYASFDYDVIGYRRTDLVRLDILVNGEPVDALSQLVFRG
                     NSQKRGRMVCERLRKEIPRQQFKIAIQAAIGGTIISRETISAFRKDVTAKCYGGDISR
                     KRKLLEKQKEGKKRMKMVGNVEIPQSAFLAVLKSDEDSN"
     gene            complement(7513..9420)
                     /gene="ftsH"
                     /locus_tag="PLF67_04735"
     CDS             complement(7513..9420)
                     /gene="ftsH"
                     /locus_tag="PLF67_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOE89356.1"
                     /translation="MKYMNDSQDQGPEKRDPNQYWQGPKKDDNNPLNRGPRVPQNRFA
                     LIALISLALVFAYVLFDGGSTRGEQVVPYTSFLAYVESNQVAQVEIKEQSQIRFTMKN
                     GLSAQSRIPYFDEDLLSILKKQGVVITGTVAEISILQVVLQLLPWIIFIGFTIMLWRQ
                     SAGINGRMMSTLGKSRAKEYMESDTKVTFKDVAGQVEAKYELEEVVAFLKNPDQFTKV
                     GAKIPRGVLLVGPPGTGKTLLAKAVAGESGVSFFHTSGSDFVEMFVGMGAARVRDLFE
                     QARKNSPCILFIDELDAVGRTRGGGLGGGNDEREQTLNQILVEMDGFSTTTGVIVMAA
                     TNRPDILDPALLRPGRFDREVVVDLPDIKEREEILKIHARKVKLEEDVDLKRLARGSA
                     GTSGADLANLVNEAALFAARKNKLTVGMAELEEARDKILLGVARKSRVMTEEEKRATA
                     YHEAGHALLHYHLKNLDPLHKVTIIPHGRALGVTISLPERDAYTKNYSSLHDWIKVTM
                     GGYVAEELVYGETTTGTSNDIKQATQIAHRMVTEWGMSDLGFINLSDESEPLFLGRQI
                     TQRKDYSEETARRIDEEMKKILDSAMAETRKILSEHRDQLDTLTEALVEHETLDDNEV
                     RALLGFTVEEK"
     gene            complement(9404..10765)
                     /gene="tilS"
                     /locus_tag="PLF67_04740"
     CDS             complement(9404..10765)
                     /gene="tilS"
                     /locus_tag="PLF67_04740"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOE89357.1"
                     /translation="MNTIERTPPEVIRTLIDKEGRIAVAFSGGADSLALLVGVCEVRD
                     PEDIVALYVNHRLRPEEELEAELVLNRKNAERLGVVFEVLDLGPGSVEDLAKERKKGV
                     EEAARTLRYQVLLRACKMHGCSTLLTGHNADDQLETLLRRLFTAGSLSSLQGIRSRLE
                     QDGITILRPVLNCPHKDLEAYLVEGGWAWSEDSTNKAETYQRNLLRRRMTGDLLSIFE
                     GAYTALEVQTRRFADLAVFLESSVEKAFEGVDVRGDEASFSLSWFQLLPSTVQELLLF
                     RLAGADSSSAFIANVHRALKADKHAIMESGSHRIEVHRGVVTWKRNEEPWSYAIEAIG
                     DEIVLPGNLVFRREAENRDSTLLRIDGTVLENPVLRLSRDGDEFAGEGGTANVAKILS
                     SQGLPPALRVPVLVDRSGIVAIFARVYGGRDRLAKRFKAPLARRLTNIYSVSRRIDDE
                     IYE"
     gene            complement(10716..11279)
                     /gene="pth"
                     /locus_tag="PLF67_04745"
     CDS             complement(10716..11279)
                     /gene="pth"
                     /locus_tag="PLF67_04745"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOE89358.1"
                     /translation="MMIILGLGNPGSKYENTRHNAGFNVTDRLAAFFHVKLRKRCFRP
                     YRQARIKDSLIIQPLTYMNRSGSVLKYFRTEKQLIVVCDQLDLPPGMIRIRRKGSSAG
                     QRGLQSIIDAHGSEDFIRIYVGIGRPERGESVVEYVLAPSDDPLLEVGIQQAAEALVK
                     LLQGFGISEVMDEYNRKNTPRSDQNPH"
     gene            complement(11279..11914)
                     /locus_tag="PLF67_04750"
     CDS             complement(11279..11914)
                     /locus_tag="PLF67_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOE89359.1"
                     /translation="MGNKRTLSASLRTEDFGSRGARRLLRSNRIPAVIYGKNDPVHIS
                     LDNQEFSNKVRHFSETALLKVMVGENEYECLMKDYQEDLIRGQIKHVDFYEVTSGQLL
                     RTMVSLVLHGTPIGVREGGVLDQVAYEAEIESLPSDLPENIQIDVSGLELNGVLTLED
                     VEFPKGVKLLSDPEGVVAIVQSIREEVEEEEEEDADEPVLVGAKKGSDEED"
     gene            complement(12072..12143)
                     /locus_tag="PLF67_04755"
     tRNA            complement(12072..12143)
                     /locus_tag="PLF67_04755"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12108..12110),aa:Gln,seq:ttg)
     gene            complement(12190..12444)
                     /gene="spoVG"
                     /locus_tag="PLF67_04760"
     CDS             complement(12190..12444)
                     /gene="spoVG"
                     /locus_tag="PLF67_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOE89360.1"
                     /translation="MEISEVRVRKVQQGGKLKAYVTVTFDDQFVVHNIKIIEGQEGNF
                     IAMPSRQLANGEFKDIVHPITSSFREELQNAIMKAYEEAD"
     gene            complement(12475..13350)
                     /locus_tag="PLF67_04765"
     CDS             complement(12475..13350)
                     /locus_tag="PLF67_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89361.1"
                     /translation="METQIVRFAPAKLNLHLAVGPLEQNGLHSIQSLFVTASLVDVLA
                     ITLSPSESFSIAVTGLEAYCLPGEDTITKAALTYQTYRPLPFALHVHCTKRIPVQAGL
                     GGGSSDGAAILLFLQELAGENKLGDEELLTAAKHVGSDLPFFVSGAKAAIVEGTGEVV
                     VPLPCVSYPALVVKPVNLNISTKNAYGSLDRIMDRPLQWADRGDIASAFKDSIPNWHK
                     TFYNDFEQTLSDLPFYRELRKLTARFDGYQGLSGSGPSWFFIAESDQKVDVLAQMIDD
                     RFGSHVAIYRLRVCS"
     gene            complement(13430..13927)
                     /locus_tag="PLF67_04770"
     CDS             complement(13430..13927)
                     /locus_tag="PLF67_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89362.1"
                     /translation="MKRTIGVVLIILINLSLFALPVALSFETWNSKAIAGPAASAGVS
                     VGVLPNLELQGAFLAELTPRIGNRIGAFGALTIGLVNPHHYRDPARAPLYYNALVGVG
                     YLGGWNMAEGKPFGEIFVRLTPLSLGSSYYRTRERTFGIGVGFDHYTHRWSFIVNMFA
                     FDRYL"
     gene            complement(13936..14409)
                     /locus_tag="PLF67_04775"
     CDS             complement(13936..14409)
                     /locus_tag="PLF67_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89363.1"
                     /translation="MKRLFSGLLILLLATSALFSAPWLGGYRLSFDGYQDRAGQYYGA
                     HLYLEPLWGTTLGLGFSVGGATSFGLSAPSSLFESSVEIRLLNVEHRLFRRPSTWRIA
                     LSGGIVTDFETFHWYAQCDPLVFFFGEKTIKVLGLRLLDDRSWALRLLEITHYFF"
     gene            complement(14413..15678)
                     /locus_tag="PLF67_04780"
     CDS             complement(14413..15678)
                     /locus_tag="PLF67_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOE89364.1"
                     /translation="MRRTTALIVLAMTFALISCTSVKTFEKRAREIIPDPIAYLVDRG
                     LPAYPSSDITLFNTGREWNVHTLKRIADAKEYILVTVFLGNIHEVSYEVWDALAEKVK
                     EGVDVYVLLDSSSYFQLTPHTSLVVPAAFNYARDIGLKVAEYNPMSVSHAAFLPLLLD
                     RDHRKIWIFDGEYLAVGGINLNEASLMIPPETGNIDTMVEIQSPQAIEALVDSFVRTW
                     ERYSVQPIDRAAFSVPPKENLPKTVYLSDHYFFADNSVTDLFDALILGAQKEVWMIQG
                     YSFLTKELINRIKEATNRGVKVNIVLSENAVKVHYEKAAFFGMVDLIDAGATLYLFDG
                     PHHAFLHCKLIVADEKYSLFGSVNYNLRSQFLSREISFLFDDEAIAEKALEHVGFIID
                     HSRIVDREEAKTHRKIDYLFYNLLMQLIG"
     gene            complement(15723..16457)
                     /locus_tag="PLF67_04785"
     CDS             complement(15723..16457)
                     /locus_tag="PLF67_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE89365.1"
                     /translation="MNLFSNAIVSRTGDFANQVDSLRFNCDCYIYNPLDYAWPMMETY
                     IRRYLSQPVKTVFLGMNPGPFGMAQTGIPFGEITVVKEYLQIEEEIGRPLVEHPKRPV
                     LGLATQRREVSGQRLWGLIQEYFPNAQDLVGSIAVINYCPLVFVDRGSTGKNITPDRL
                     GLGERMALEAICDSYLHDMLTMLQPAFALGVGKYAENKLITVAQAFDDITVGSILHPS
                     PINPKANQGWAGIVRAQLDELGVFHE"
     gene            16676..18400
                     /locus_tag="PLF67_04790"
     CDS             16676..18400
                     /locus_tag="PLF67_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE89366.1"
                     /translation="MQELIMNHSWDVLDQYRGGLFEGTWPAILDLLSITKEKFPKRNG
                     FTVFNPERTTLTWEEVWSEVRRLGSYLIEAGIKADDKVVINGKNSPAWALSYFAALWA
                     GAVVVPLDNQMPIDRVHALGEFVEASFVFADAAIINQLNKEAPWVKNLKGIVSLTNPT
                     AAAVNYTTLRPKKMVERVKRTEHDLAAILFTSGTTGNEKGAMLTHGNLVSDVFQACDG
                     IFLQISEVDVLYALLPLHHSYSCTAVLLESITHGSECVFGHDIVVSKMINDMKRGKIT
                     VFMGIPLLYNKILAGMMKQVRKKGVATYALIRTMMKVNGFFKKHLKWNPLRKSFDKLL
                     LSKLGLDHNRICICGAGPLSPKVFTQYQQLGIDFIQGYGLTETAPILTLNPVSRFKVE
                     SVGLVFPLVDMIIADKDDSGVGEVRVKGPNVTSGYYKDPEHTAELFDEEGYLKTGDLG
                     YLDKENYLYLKGRVKNVIVTEGGKNVYPEEIEDLFQLYDQVEQILIRGYQEEKDIPSE
                     LIEAVIYPSQEYFEGKEGEVQKELDRVITEVNQQLAAYKKITRLTIIDKPMDMTSTKK
                     IKRNKVTV"
     gene            18424..19698
                     /locus_tag="PLF67_04795"
     CDS             18424..19698
                     /locus_tag="PLF67_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOE89367.1"
                     /translation="MKTLIANALIIPMDREGHFVVGDIGLDGPAIAFVGNKDPSFTAD
                     RVIDAQGYIALPSLYNSHTHLAMGLMRNYKDNEPTLQAWLNEIFPIENKLVPEDILVS
                     SRIGIIEALQSGTGFFHDMYFKAEATAQAVKESGMRAKPGLTLFGDLADSKERLKRWD
                     AKVLPLIDDPITYSIAPHAVYTCSKETLRFAHDAAKERGVPLHIHLSETEKENSDCLF
                     EHRKTPLQYLIDLNFFDDVFAFLAHCVHLSEEDRKLLKDLPAAVVHNPSSNLKLASGI
                     APVATYLEQGIPVLLGTDGASSNNNLNLIEEMHLAALLGRLSSKDGLPLKPYQVLEMA
                     TSSAAKAFGIKGGMLQAGFESDLILINTKKAHLAPLNDPFSALVYSVQGSDVEYLFSR
                     GELLLEKGRPTRFDLPSSIEAINDAWADIKQR"
     gene            19711..20742
                     /gene="mtnA"
                     /locus_tag="PLF67_04800"
     CDS             19711..20742
                     /gene="mtnA"
                     /locus_tag="PLF67_04800"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOE89368.1"
                     /translation="MDERFEALLFDAETLILLDQRVLPTEETYHHCRTTAEVEFAIRD
                     MVVRGAPAIGAAAAYGVYFAAKEAKGEKEFNELCSFLGNARPTAVNLEWAIRRMQAIY
                     QTNKTRNDLLGLLLEEAHEIRREDIATNKAMAKIGNAVVPHGATILTHCNTGALATAG
                     WGTALGVIKEAFYAGKEIFVYADETRPRFQGARLTAWELMEAKIPSKLIADSAAATLI
                     RDGKIDLVILGGDRVAANGDVANKLGTFMLSVICKAYGVPFYSVVPVSTIDFDLESGE
                     LIPIEERDPSEVTHVGDTQVAPTGMDVYNPAFDVTPHQNITGIITERGIIYPPFKENI
                     ARLKRGEHL"
     gene            20794..21180
                     /locus_tag="PLF67_04805"
     CDS             20794..21180
                     /locus_tag="PLF67_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOE89369.1"
                     /translation="MKKEVVFYRCKRCGNVARKDYDMGPPLVCCGEEMVALKANTQDA
                     AQEKHVPVVKVEGNRLTVDVGSVEHPMVAEHHIPFIYVETKNGGLRADLQVGSKPHAT
                     FTIDANDKAVAVYEYCNLHGLWKFVL"
     gene            complement(21177..21878)
                     /locus_tag="PLF67_04810"
     CDS             complement(21177..21878)
                     /locus_tag="PLF67_04810"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89370.1"
                     /translation="MRFSNVSKAYGDHVVFDRFSFALDPKTIMSVVGPSGGGKTTLLR
                     LAAGLVEPDGGTVEKDEGKVSFLFQEPRLLPHVNVWANVEITLRAFYEQEERTRIALH
                     YLELAEMKESLHLYPHELSGGMRQRVAIARAFAHPAELMLLDEPFQSLDIRLRMHLIK
                     AFLTLWEEEKRTTIFVTHDTKEAVLIGDRVLCLGDPHTPRMDEQIKIPRTARDIHDPV
                     LMALEAKLFGLLTRF"
     gene            complement(21856..22647)
                     /locus_tag="PLF67_04815"
     CDS             complement(21856..22647)
                     /locus_tag="PLF67_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE89371.1"
                     /translation="MNRTRRNLILLFSSLGLLLLWQGIAKSVDIPVIMPSLGPIVRAL
                     GELASGPAFSANLLATILRAIRSFLIIVITGTVLGLIGGRNQTVSLILKPFVTTLKAT
                     PVMSVILLAFIWFSSGSVPLFSAFLMGFPVMYLQIEHGVKQLDPNLGEMCDLYDVPPK
                     TKLFYYTLPSLAPWFVAGSRATLSMIWKVVIAAEVLTVPRHGVGSRMQLAQVQLQTDV
                     VLSWTLVAIALTALGDLFFEGVVSVVAKTRSTVQRRRVDALLKRF"
     gene            complement(22656..23594)
                     /locus_tag="PLF67_04820"
     CDS             complement(22656..23594)
                     /locus_tag="PLF67_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89372.1"
                     /translation="MKKYVLMLIAILVIVVGLFGQPIREADPGTIRLGVMAGPTGFSS
                     AGLDQPDVELQVFPSPNEVIARLANGELDMAALPTNVAANLYNKGVEIRLAAVIGNGM
                     LSLISSKGGTDIEDLYGATLHLPGAGSTPDQMARMLLAWAGLVEGKDIFFDYSVAAPA
                     QLAQLLIAGKVDYALLPQPFVSMVLNRSVQATQLLDVQELYAELTGSPTYPMSVLVVS
                     EKFASTYPASFRKVLSGYKDSVNWVNGNPDLAARKIEELGIMAKALAQSAIPHCNLVY
                     VPASEAKSAVTFYFEQLYSLDPASIGGSVPGSELYL"
     gene            complement(23608..24867)
                     /gene="tyrS"
                     /locus_tag="PLF67_04825"
     CDS             complement(23608..24867)
                     /gene="tyrS"
                     /locus_tag="PLF67_04825"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOE89373.1"
                     /translation="MNSALQTLFDRGFIKACTNLEGLSDLMDKESIRMYVGVDATGKS
                     LHIGHMVPFFALHHLQKAGHQPIALVGGGTTMIGDPSGKSEMRKMLSKEEILSNSESM
                     KSQLQTVVDFSEHPGEGMGRAMMLNNADWLTELNYIDFLREIGRHFSVNRMLSFEAYK
                     QRMERGLSFIEFNYQLLQSYDFYILNKDHDCRLQVGGDDQWGNIVSGVELIRKMGGPE
                     CFGLTFNLITRADGHKMGKSEKGAVFLDPELYSPYDFYQYWRNVNDEDTIRFLKLFTF
                     LSLEEIATYEKEGVNINDAKERLAYEQTKIIHGEEEANKARTAALAFFGSKADTLENR
                     DGMPSITIAMSELEAGIGVLELFARTDLASTNSEARRLVQGGGAWIGDQKVDDPRAVI
                     DPSFLDEHDELILRSGKKRYFRVIVER"
     gene            complement(24869..26200)
                     /locus_tag="PLF67_04830"
     CDS             complement(24869..26200)
                     /locus_tag="PLF67_04830"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOE89374.1"
                     /translation="MAEVTLEKIVSLSKRRGFVFQSSEIYGGLNGAYDYGPLGVQLKN
                     NIRDLWWKEMTQIHEDIVGLDASILMHPRVWEASGHVANFSDPMVDCKQCKSRFRTDQ
                     IDLAAACPVCGTKDSFTEPRNFNLMFSTHIGAASDAGSLIYLRPETAQGIYTEFKNVV
                     SSSRVKIPFGIAQVGKAFRNEITTRHFIFRSVEFEQMEMQWFCAPGTDDEWFEYWKAQ
                     RFAFYERLGIRKSHLRWHQHGKDELAFYAKDAYDIQYLFPMGWQELEGVHNRTDYDLS
                     QHQEFSGKDQTYLDPVTNERYIPYVVETSAGLTRNVLMVLSDAYEEETLEDGETRTVL
                     RFHPNVAPVTVAVLPLVKKDGIAEVAKELELSLREEFTTFFDLSGAIGRRYRRMDEIG
                     TPYCITVDYQSLDDKTVTLRYRDSMEQVRIGMDEIVSAIKEANKNYKRVEK"
     gene            complement(26202..27671)
                     /gene="gltX"
                     /locus_tag="PLF67_04835"
     CDS             complement(26202..27671)
                     /gene="gltX"
                     /locus_tag="PLF67_04835"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOE89375.1"
                     /translation="MQVRVRYAPSPTGLQHIGGIRTALFNYFFARSQKGSFILRIEDT
                     DQERYSTEALDDLYATLEWLGIEYDEGPIKGGPFAPYVQSERFDIYEMYAKKLVEDGK
                     AYYCYCSPERLERVRKEQQEAKSQYQGYDRHCRNLSSEERLAYEEQGIRPVIRLKVPT
                     EGKTSFEDILMGTITRRNSDVSPDPVLLKSDGFPTYHLANVIDDHMMEITHIMRAQEW
                     IPSGPLHILLYEAFGWEPPKYCHLPMVMGKDGQKLSKRHGSTAVAEFRKEGYLPEALL
                     NYVSLVGWSYDGTREFFTKEELEQLFSLEKINKAPGVFDYKKLDWFNGQYIRMKSDEE
                     LLDLLMPYLAETGWPLTDRAKLLHLTAVAKERLKVLGDITGLARFLFEEPVWEDLSIF
                     AAKGVELPLAHTALERAYTVLLSGLEAKKEQSVIEEELVQLAKDLGMKVNGVFQPLRV
                     AITGSTVSLPLFDSIELLGFDHTMNRIQDALNVLKKGIE"
BASE COUNT         6182 a         7949 c         7371 g         6241 t
ORIGIN      
        1 tgaagtcgtg gttgccgggc gctaccgcgt catagccgag catatccaga aggacgccta
       61 ccgtctctcc ttcgaaaaga ttcgccaagt ttgttccgtg cgaaacatct ccggcatcca
      121 gaagaaggat gttatccgtg atggagcgcc ctacttccag catcgtggcc agtttggaat
      181 agcccattcc gccatcgctg ggagttatgc gcgcgtgcac gtcgttggtg tggacgacaa
      241 agagatcgaa atccttatcc cccgcactgt tcttgacgac cgacattact cctaaagggt
      301 actcaaccgc ctccacttta gccggtttta cttccgccac gacaggtgca ggaaccggtg
      361 ccggtgcagg ttcaggtgca ggagccggtt tttcttcagc tacgaccact tcctttacat
      421 catcaccaag catctcggcg gtaagcttct tcgtgctcag gtatttgatc aggacgtcac
      481 ttaagaggtc gccctcgacg aggattcgac cgaacatcgt gtatccgtct ccgccggcgg
      541 ccaggaagtc attggtcgct acgcggtagg tcttgttctt gtcaatcgcc ttgccgttga
      601 gcaggactct gacgatgcgc ttgccgggct cggcaaagcg gttgtacacg acgctcagat
      661 ccgtctgagc gaatccgccg ttcgtttccg gcagcattcg atacccatgc tcaagagccg
      721 catatacctc ggcgccggtc gcttcgacca ccgtgaggat gttcgtaaac ggcagcacgt
      781 tgatcacatc accgatcgtt accggaccgg ccttcaggga agctcggatt ccgccaccgt
      841 tggtgatcgt gaagtcggct ccgctctgtt cggtcatcgc agcggtgatg atcttcgaaa
      901 gattcgtcgg gcgggttcga acatgggccc gctcaccgtt caagtcaacc ggaatcgtag
      961 cgatgacctg gttgagcttg tcgctgagtt gagcctgcat ataggttaca tactcatcca
     1021 ctttgagatg gttcggcacg tccacgattc cataggcccg ggcgagatcg ctctcttcag
     1081 ggttgaggac atcttcggcg ggaatcagca tcggggtcgt tccgaccact ttgccgcctc
     1141 ggacgttgat ctcgacgact ccgacgttct ccagatattc acctgcggag acgatgtagc
     1201 tgtcgccgat cttaagaccc ttctccagcg catcgtggga gtgcccgtcg acaaacaggt
     1261 cgattccctt gatcttgctg acgatcatgt cactggtcag tccgctcgcc ccgtccggat
     1321 caaggccgat atggccaaga acgatgacgt agtccacgta ggtgcgggcg atatcgatcg
     1381 cctgctgacc gatggcgaag atctcttcgt tgaggatggt gagacccgcg gtattcttcg
     1441 gatggctctt cgtcttcgta tcgggggtcg tcagaccgac gactccgacg gtgaagccgt
     1501 tgaagtcata gacctgatac ggctggaata caaggtatcc atcctcgtcg agcacgttgg
     1561 cggcgaggac tcggagattc gagtactctt cggcgaactc ggcggcttga atcaggcgct
     1621 cggacccgta gttgaagtcg tggttgccgg ggacgaccgc atcatagccg agcatgtcca
     1681 aaaggactcc tacaacctcg ccttcaaaca ggttcgccag attcgttccg tgcgaaacgt
     1741 cgccggcatc cagcacgaga atgttgtccg tgatggagcg gcccacttcc agcatcgtgg
     1801 ccagtttgga atagcccatt ccgccgtcgc tgggagtgat ccggccgtgc acgtcgttgg
     1861 tgtggacgac gaacagatcg aagtccttgt cacctgcggc attcttgacg atcgaggtga
     1921 cgcccaaggg gaactctctc ggttcgaccg gtcgggtgat cttgattggt accacctctt
     1981 ccacgacagg agcgggagcc ggcggtgccg gaggcggagc gggagccgga gctggaggcg
     2041 gtgccggctt ctcttcgact ttgaccaccg gtacgggtac gacaaccgct actttctcgg
     2101 gagtcggtcc gatttgttcc acgatcgccg gatcttcggg gagcgttacc atcagggtat
     2161 agcccagcgc attggcaaag ggctcatatt ccgcggcaag ggggctcaaa tagggaacag
     2221 caaggtcttc tgcgggcaga acgagcggca ggacgccggt tacgcggccg cggtcaacgc
     2281 tgaccaccac gccgccgatt gagcgcagct gctcttcggc gcggacgatg agggtgtcgt
     2341 tgacgacttt cgctgtctcc gaaccgctgc catcgacgat caggtcgata ccttgaatgt
     2401 tctctgccag atcggacgaa ctgaacacgc cttcaccgag atcggtaagg acgacgaggt
     2461 agtccacata ggtgcgggca atgtcgacgg cccactgggc gttgtcgagg atgacatcgg
     2521 cgtcaaagct tactccgctt accggtttcg gcgccacgat gccgacaacc ccaacggtga
     2581 agccgttgaa atcatagact tggtagggct ggaagagcag gtactcttcg gcatccaaca
     2641 cgttcgcggc gagggggatc gccttttggg ttccttcgat cccgagctca atctgaatcg
     2701 cttgcggcag atatgcatca tagcccagct catcgacgat ccatgctgcg gtaagggccg
     2761 cttcggcggg aatctctccg acatagccgg tgttgagcaa caaccagttg tcggtaatct
     2821 cacgaccggc cttcagggcg gtggagagct tggcgattcc gactccgccg ttctcagcat
     2881 agatattgcc cttcacgtca ttggtgtgaa cgatgaaaag atcgaacact tcatttccat
     2941 cagcgttctt aacgattgga cggactccat agggatagac gacctcctcc actacggggg
     3001 gagcgactac cacgggagcc ggttcaacag ccactggctt aacaggctca acaaccatcg
     3061 gcacctctac aacaggtgcg ggttccggtg ctgggactgc tggtggtggt gccggagccg
     3121 ggacgggctc aaccactgct ggaggaattg gctcaactcg cgctgtcgca cagcttgcga
     3181 gtgaaaatgc caacaatgtg accagcaaag ccgtcaaaat tgtgcttgaa aaacgtttca
     3241 cggttttcct cctcttgaat ttgcatgtcg ttgcataaat gcgtacagta actatatcac
     3301 accaaattga agtgcgatac cgtaaacatc actttttgtg taacgatatc ccctcaaatc
     3361 tttctataat ggaccaaaaa tcgggtaaac gtgcctttat cacgggaatt tttagtgact
     3421 tggcgaagct cggataacaa aaaacccata gatcgttttc tacgggttcc ttccaattcc
     3481 gcaatgaatt acatcaaatc tttcggcttg cgggcggtgt tgccggattt ctcacagtac
     3541 gccatatgcc gcaacttgcc gttttcaaag accgatttca tcttgatcgt tccgccccag
     3601 cgactggtca tcaccttggt tccttcacgc cgggcgttct ttgatacgtt tcctgccata
     3661 tgccattcct ccaatcaaac ttcaattcaa cactcttact gtactcgttt ttcccatgct
     3721 agtcaaatcc ccccgccttg catacaatac ggttcatgaa gcgttttctc cgccaactct
     3781 ttctcatccc cgtctatttg tacaaaggac ttctctcccc gttcttcggc tcctcctgca
     3841 tctatcatcc gagttgctcc acctatatgg tcgaggcggt gctgcgccat ggaatcataa
     3901 aaggatcgat catgggaatc gcccgaatcc tccgctgctc gcggtggttt ctcggcggag
     3961 aagatcccgt acccgaagat tggtcattta aagcgatcaa ggatgagttc acacgtcatc
     4021 gtcggcgctc gtgatcccct tgacgaatgg aatcagcacg ctcaaggcgc tttcgttgta
     4081 tcccccttcg ggaatgatca gatcggcgta cactttcgtc ggttcgatga agttgaagtg
     4141 tcccggccgg acgacctcca gatactgggc gcacacgctc tccagagtcc ttccccgctc
     4201 ggagatgtcc cgcttcaggc ggcggatgaa gcgaatgtca tccggagtat ccacgtacag
     4261 cttcaaatcc aacagcgccc gaatctccgg atcgtgaaga accatcaacc cttcaataat
     4321 caccaacggc cgaggatcga catgcaccgt ttcatcccgg cgacggtggt tcacaaaatc
     4381 atacaaaggc atcttgatgg gccgatatgc cttcaagtcg gagaggtgcc tgtaaagcag
     4441 atccatgtcg aacgcatcgg gatgatcgaa attgaacgca gtgatattct catttgaaat
     4501 ataggctgcg gatttatagt agttgtcctg aggaatgaag acgaagtcgg aacagacctc
     4561 gctgatcttc cgcacaatcg tcgatttccc cgaacccgat cctccggtga ttccgatgat
     4621 tttaacctct ttcatctcgc tctcactttg ttgaaaatgc gaataccgtc gtatggcgat
     4681 agggtctgtc gggaccgaag atcgcgttgg ggaaatgcgc ctggttgggt gaatccggat
     4741 agtactgcgt ttcgaagcac atgcccgcat gtttttgata gggcacgccc cccttgccga
     4801 ccgtacttcc gtcaaggaag ttgctcgtat aaaactgcaa agacggcaac gtggtgaaga
     4861 tctccatcat ccggccgctg cccggatcat ataccgtcgc cgccgggcgg agctcggacc
     4921 gctcatcgct caaaatgaag cagtggtcgt atccgcccgc cgtttcaatg tccaagccga
     4981 ccgctttgga tttcctgaaa tcaaaaggcg tctgttcgac tccaagaacc ttgccggtgg
     5041 gaatcgcatg gtcatcgact tcaacatacc ggtcgcagaa cagttggaca tcatgatgca
     5101 gaatcgtgcc gcttccggcg cctgcgaggt tgaagtagct gtggttcgtg aggttcatcg
     5161 gggtctcttc gcttgccgtc gcttcatact caagtcgcag ctgtccgctc tctccgtcca
     5221 gcaggtagct taccgttgcc gttacgctgc cgggaaaccc tccttcaccc gcctgggaag
     5281 tgatagaaag aacaacgccg ggcttctctt cccattcaaa gggctcgatc gcccagagcc
     5341 gccaacccca gtttgcggaa ccgctgtgca gcgagttctt tccatcgttg gcgtcaagct
     5401 tgtaggtgcg gtcattgagg gtgaacgtcg catccttgat ccggttggcg aaacgaccga
     5461 cgacttgccc gaaatacgcc gaatggccga gatattcaaa aggattttcg aaacccaaga
     5521 cgatatcccg cttctttccg tcccggtcgg gaacgatcag ggattggatc gtcgctccca
     5581 gattgagaat ctgaacggtg tacggacctg actccaaggt taccttgaag atgtcctccc
     5641 ccttcggact ttctgcgatc ttttgaacgt gtaccatggc tcctcctcga cttcaattgg
     5701 aatcctcatc actttttaac acggcaagga acgctgattg tggaatttca acgtttccga
     5761 ccatcttcat ccgcttcttg ccttccttct gtttttccag cagcttgcgc ttgcgagaga
     5821 tatcgccgcc atagcacttc gccgtcacat ccttgcggaa cgcactgatc gtctccctgc
     5881 tgatgatagt accaccgatt gccgcctgga tggcaatttt aaattgctgg cggggaatct
     5941 ccttgcgcag gcgttcgcac accatccgtc cccgtttttg gctgttgccg cggaagacga
     6001 gctggctgag cgcatcaacc ggctcgccgt tcaccagaat gtccaatcga accagatcgg
     6061 ttcgccggta cccgatgaca tcgtagtcaa aggaggcata gccgcggctg acggatttga
     6121 ggcgatcgta gaattcaaaa aggacctcgg caagcggcat ctcgtagatc agttcaaccc
     6181 gtttcgcatc aaggtagttc atccccacct gcgttcccct tcgatcgatg cacaggttga
     6241 tgatcggtcc gaggtatgaa acaggagtga tgatgttcgc ttgaatgtac ggctcttcgg
     6301 cgtaatcgat tttcatcggt tcggggtatt ccaacgggtt gtcgatcgag agcatcgacc
     6361 cgtctttcat atgaacatgg tattggaccg agggactggt gaagacgatg gagagaccga
     6421 actcccgttc gatccgttcc tggaccacct cgaggtgaag catccccaga aatccgcacc
     6481 ggaagccgaa gcccaacgcc gcggaggagt ctttttcgta caccagggaa gcatcgttca
     6541 gcttgaggcg gtcgagcgca tcgaccagct cctcatagtc gttcgtatcg acgggataga
     6601 ttgagctgaa caccaccggt ttgacatcct tgaaccccgg cagcggccgc ttcgccggtt
     6661 ttgcagccga cgtgatcgta tccccgaccc ggacatcgct gatcgtcttc accccggtaa
     6721 tcacgtaccc gacatccccg gcggagagct tgtcgcgctt ttgcaggccg agctggaaca
     6781 atccgacctc ttccgcccga taagtggtgt cggtatgcat gaacgtgact tcatcaccgt
     6841 ctttcagctc gccgttgaag attctacagt gaaccaccac tccgcggtac ggatcatagt
     6901 gggaatcgaa gatcagcgcc tccaaggagg ccttggggtc tccgctcggc ggggagatgt
     6961 ggttgacgat cgcctcaaac agttcgccca acccgaaacc ggtcttggcg ctgacttgga
     7021 ccgccatatc ggggtccaac ccgagatcat gatcgatctg gtggaggcac tcctcgatat
     7081 ccgccgaagg caggtcgacc ttgttgatca ccgggatgat gtcaagattg tgctccatcg
     7141 ccatatagag gttcgcaagg gtctgggctt ccaccccctg ggcggcatcg atcagaagga
     7201 gcgccccctc gcaggagctg atcgcccgac tcacctcata ggagaagtcc acgtgtccgg
     7261 gcgtatcgac caggttcaat tcataggttt tcccatcctg agctttgtat ggaatcatga
     7321 ccgcctggct tttgattgtg atgccccgct cccgctcgat atccatattg tcgaggatct
     7381 gggtctgttc cggaccgcgg ttctgacgga tctgcgcccg ctcgatgaag cggtcggcaa
     7441 gcgtcgactt tccgtggtcg atatgcgcga tgatgcagaa atttctcttc aaactggaat
     7501 ccaaggtcat atctatttct cttcaacggt aaaacccaac aaggcccgaa cctcattgtc
     7561 atcaagggtt tcatgttcga cgagcgcctc ggtcagggtg tcgagctggt cgcgatgctc
     7621 gctgagaatc ttcctcgtct cggccatcgc cgaatcgagg atcttcttca tctcctcgtc
     7681 gattcgacgg gcggtctctt cgctgtagtc tttccgttga gtgatttgcc gcccaaggaa
     7741 gagcggctcg ctttcatcgg aaaggttgat gaatccaaga tcgctcatcc cccactccgt
     7801 caccatccgg tgggcgatct gggttgcctg cttgatgtcg ttgctcgttc ccgtcgtggt
     7861 ctcgccgtag acgagctctt cggcgacata cccgcccatc gtcactttga tccagtcatg
     7921 cagggaggag tagttcttgg tatacgcatc ccgttccggc aagctgatcg taaccccgag
     7981 cgcccggcca tggggaatga tcgttacctt gtggagggga tcaagattct tcagatgata
     8041 atgcaagaga gcgtgccccg cttcatgata ggcggtcgct cgtttctcct cctccgtcat
     8101 cacccgcgat ttccgggcca ccccgagaag aatcttgtcc cgagcctcct caagctccgc
     8161 catgccgact gtcagtttat tctttcgggc ggcaaacaga gccgcttcgt tgaccaggtt
     8221 cgcaagatcc gctccgctgg tgccagccga gccgcgggca aggcgcttca gatcgacatc
     8281 ctcctccagc ttcactttgc gggcgtgaat cttgaggatc tcttcccgct ccttgatatc
     8341 gggaagatcg acgacgactt cccggtcaaa tcttccgggg cgcagaagcg cagggtcgag
     8401 aatatccggc cggttggtcg ccgccatgac gatgacgccg gtggtcgtcg aaaaaccgtc
     8461 catctcgacg aggatctggt tgagtgtctg ttcccgttca tcgtttccac cgcccagacc
     8521 gccgccgcgg gttcgtccaa ccgcatcgag ctcatcgatg aagaggatgc agggactgtt
     8581 cttgcgggcc tgttcaaaga gatcacgaac tcgggccgct cccattccga cgaacatctc
     8641 gacgaaatcg gagccgctgg tgtggaagaa ggaaacgccg ctctcgccgg caaccgcctt
     8701 ggcaagaagg gtttttcccg ttccgggagg tccgaccaga agaacaccgc gggggatctt
     8761 tgcccccacc ttggtgaact gatcggggtt cttcaggaac gccaccactt cttcaagctc
     8821 gtatttcgcc tctacttgac cggcaacatc cttgaacgtg actttcgtgt cggattccat
     8881 atattccttg gcccggcttt tacccaaggt ggacatcatc cgcccgttga tcccggcgct
     8941 ttggcgccac agcatgatcg tgaaaccgat gaagataatc cacggcagca gctgaagtac
     9001 gacctgaaga atcgaaatct ctgcaaccgt tccggtgatt acgactccct gttttttcag
     9061 gatcgacaga agatcctcgt caaaatacgg tatccggctc tgggcggaaa gcccgttctt
     9121 catcgtgaag cggatctgcg actgctcctt tatttcaact tgggcgacct gattgctctc
     9181 cacatacgct aaaaaagagg tatatggaac gacctgctcg ccgcgggtgg aaccgccgtc
     9241 aaaaagaacg tatgcgaata caagagcaag gctgatcaaa gcgatgaggg caaacctgtt
     9301 ttggggcacc cttggaccgc gattcagagg attgttgtcg tccttcttcg gaccctgcca
     9361 atactggttc ggatcccgct tttcaggacc ttgatcctga ctgtcattca tatatttcat
     9421 catctatcct tctgctcaca ctatatatat ttgtcaaccg gcgggcaagg ggagctttaa
     9481 agcgtttggc caatcgatcc cgccctccgt agaccctggc gaaaatcgcg acaattccgc
     9541 ttcgatccac caatacggga accctcaagg ccggcggcaa tccctggctt gaaaggattt
     9601 tcgccacgtt cgccgttccc ccttcccctg cgaactcatc gccgtcccgg ctgagccgaa
     9661 gaaccgggtt ctccaacacc gttccgtcga ttcgaagcag cgttgaatcc ctgttttccg
     9721 cttccctccg aaagaccaga ttccccggca gaacgatctc atccccgatc gcttcgatcg
     9781 cataggacca aggctcctcg ttccgtttcc acgtaacgac cccccgatgc acctcgatgc
     9841 ggtggcttcc gctttccatg atggcgtgct tgtcggcctt cagagccctg tgcacgttcg
     9901 caatgaatgc ggaggagctg tccgccccgg ccagccggaa gaggagaagc tcttgaaccg
     9961 tcgaaggcag aagctgaaac caggaaaggg aaaacgatgc ttcgtctccc cgaacgtcaa
    10021 caccttcaaa cgctttttcc acggatgact caagaaacac ggcaaggtcg gcaaaacgcc
    10081 tggtctgtac ttccaaagcg gtgtacgcgc cttcaaagat tgaaagaagg tcgcccgtca
    10141 tccgtcttcg aagaagattt cgttgatagg tctccgcttt gttggttgaa tcttccgacc
    10201 acgcccatcc gccttcgaca agatatgctt caaggtcttt gtgaggacaa ttcaatacag
    10261 gtcgaagaat cgtgattccg tcttgttcca gcctgctcct gatcccctgc agggatgaaa
    10321 ggctccctgc ggtgaagagg cgcctcaaca gcgtttccag ctgatcatcg gcgttgtggc
    10381 cggtaagcaa cgttgagcag ccgtgcattt tacatgcgcg aagcaacacc tgatagcgca
    10441 atgtccgggc agcctcctcg acccccttct ttcgctcctt tgcaagatct tcgaccgaac
    10501 cgggtccgag gtcgagcacc tcgaagacca cgccgagtcg ctcggcgttc ttcctgttga
    10561 gcaccagttc cgcttccagc tcttcttcag gacgcaagcg gtggttcaca tacagagcga
    10621 cgatatcttc gggatctcga acctcgcaca ccccgaccag cagagcgagc gaatccgcgc
    10681 cgccgctgaa ggccacggcg attcttccct ccttgtcaat gagggttctg atcacttcgg
    10741 ggggtgttct ttcgattgta ttcatccatc acctccgata tcccaaatcc ctgcagaagc
    10801 ttgacaagcg cctcggccgc ttgttgtatc cccacctcca aaagggggtc gtcgctcgga
    10861 gcaaggacgt attccacgac ggattctccc cgttcaggtc ggccaatacc gacatatatg
    10921 cgaatgaaat cctcactgcc atgcgcatca atgatcgatt gaagcccccg ctgtcccgcc
    10981 gacgatcctt ttcgccggat ccgaatcatc cccggcggca gatcgagctg gtcacagacg
    11041 acgatcagct gtttttccgt ccggaaatat ttgagcacgg aaccgcttcg attcatatag
    11101 gtaagcggct gaatgatcag tgaatccttg atgcgggctt gtcggtaggg acgaaaacag
    11161 cgttttctca acttcacgtg aaaaaacgct gctaaacggt cggttacatt gaaccctgca
    11221 ttgtggcgag tattctcgta cttggatccg ggattcccca acccaagaat aatcatcatt
    11281 aatcttcctc gtcagaccct tttttggcac cgacaagaac aggttcatcg gcatcctctt
    11341 cctcttcttc ctcaacctct tcccgaatcg actggacgat tgcgacaacc ccttcgggat
    11401 cgctgaggag cttgacgccc ttggggaact cgacatcttc aagggtcaat acaccattga
    11461 gctcaagacc ggaaacatcg atctggatgt tctcgggcaa gtcggagggc aatgactcga
    11521 tttccgcctc ataggcgacc tgatcgagaa ctccgccttc acggacaccg atcggggtcc
    11581 cgtgcagcac gaggctgacc atcgtccgaa gaagctgacc ggaggtaact tcataaaaat
    11641 cgacgtgctt gatctgaccg cggataagat cttcctgata atccttcatc aagcattcgt
    11701 actcgttctc ccctaccatg accttcagga gcgccgtttc cgaaaaatgc cgtactttgt
    11761 tgctgaactc ctgattgtcc aagctgatat ggacgggatc attctttcca taaatgactg
    11821 ccgggattcg attgcttcgg aggagtcttc gtgctccgcg acttccgaaa tcttccgttc
    11881 tgagtgaggc actcagtgtt cgcttattac ccattgttta ctcctgtact ctcattggcc
    11941 gattgccaat taccatgaca ttagaggata tgcaacacat atcgccaaga accgagtata
    12001 gaaaacttat tcccttctat acaaggccct cgtaaaagaa aagggcaatc gatccacagg
    12061 attgccccaa actgggaagg agggactcga acccccggtg ccggcaccaa aaaccagagc
    12121 cttaaccact tggccacttc ccaataacgg ttagacccta tcgtaaaaaa accgttttgt
    12181 ccagtaaggt taatccgctt cctcgtacgc cttcatgatc gcattctgca gctcttcgcg
    12241 gaagctgctg gtgattggat gaacgatatc tttaaactcc ccgttggcca gctgccggga
    12301 gggcatcgca atgaaattgc cctcctggcc ttcaatgatc ttgatgttgt gaaccacgaa
    12361 ttggtcatcg aaggtaaccg ttacgtatgc cttcagcttg ccaccttgct gcactttgcg
    12421 aactcgaact tctgaaatct ccacactaac ctccgcggaa ctgtatcaaa tcacctacga
    12481 gcacaccctc aggcgataga tggctacgtg agaaccgaac cgatcatcga tcatttgtgc
    12541 aagtacatcg actttttgat cggattctgc aatgaaaaac caagatggtc cacttccact
    12601 caacccttgg tagccgtcaa accttgccgt aagtttcctc aattccctgt aaaaaggaag
    12661 atcggaaagt gtctgctcga aatcattata aaacgtttta tgccaatttg gtattgaatc
    12721 tttaaatgct gaagcgatat ctccgcgatc tgcccattga aggggtcgat ccatgattcg
    12781 atccagggaa ccataggcgt ttttcgtcga tatattcaga ttgaccggtt ttaccaccaa
    12841 tgcgggatat gaaacgcagg gaagcggaac gaccacttct ccggtgcctt caacgatcgc
    12901 cgccttcgct ccgctgacaa agaaaggaag atcgctgcca acgtgttttg ccgccgtgag
    12961 aagctcttca tcaccgagct tgttctcccc ggcaagttcc tggagaaaca gcaggatcgc
    13021 cgctccgtca ctggagccgc cgccaagccc ggcttgaacg ggaatccgct tggtgcagtg
    13081 aacatgcaga gcaaagggaa gcgggcggta tgtttgatag gtcaaagcag ctttggtgat
    13141 cgtgtcctct ccgggcaggc agtacgcttc gaggccggtt acggcgatgg agaagctttc
    13201 actcggtgaa agggttatcg cgagaacatc aaccaaagaa gcagtcacaa agagagactg
    13261 aattgaatgg agtccattct gctcgagagg cccgaccgcc aaatggagat tgagcttcgc
    13321 cggagcaaat cggacgattt gcgtttccat accgcccaga gtagcaaagc atggtggaac
    13381 tgtccagtac aagacataaa atttccttgc cgatggtgca tattggcatc tataagtaac
    13441 ggtcaaaggc aaacatgttc acgataaagg accaacgatg ggtgtaatgg tcaaaaccga
    13501 cgccaatacc gaacgttcgt tcccgggtgc gataatacga tgaacccaat gagagcggcg
    13561 tcagtcggac aaagatctcc ccgaacggtt ttccttccgc catgttccat ccaccgagat
    13621 accccactcc cacgagcgcg ttgtaataga gcggagctct ggccggatca cgatagtgat
    13681 gcggattcac cagaccgatg gtgagcgctc cgaaagcccc gatccggttt ccgatccgtg
    13741 gggtcagctc cgccaaaaac gctccttgaa gctcaagatt cggaagaact cccacactga
    13801 ctcccgcact tgccgccgga ccggcgatcg ctttggagtt ccacgtctcg aacgaaaggg
    13861 cgacgggcag tgcaaagagg ctcaagttta tcaggatgat gagcacaaca ccgattgttc
    13921 gtttcacacc gcctcctaaa agaaataatg ggtgatttcc aacaggcgca gcgcccacga
    13981 gcgatcgtca aggaggcgaa gccccaacac cttgatcgtc ttttctccaa agaagaacac
    14041 aagcggatcg cactgggcat accaatggaa ggtttcaaag tcggtaacga tccctccgct
    14101 taaggcgatt cgccacgtcg aagggcgccg gaacagcctg tgctcaacat tgaggagacg
    14161 gatttcaacc gaactctcaa agagggaaga aggggcggag agaccgaagc tcgtcgcccc
    14221 cccgacgctg aagcccaaac cgagggttgt tccccaaagc ggctcaaggt agagatgagc
    14281 gccgtagtat tgccctgccc gatcctgata cccgtcaaag ctcaagcgat agccgccgag
    14341 ccagggagcg ctgaagagag cgcttgtcgc gagaagaagg atcagcaagc ccgaaaacaa
    14401 tcgtttcata ccctacccta tgagctgcat cagaagattg tagaagaggt aatcgatctt
    14461 ccgatgcgtt tttgcttctt cccggtcgac gatccggctg tggtcgatga tgaatcccac
    14521 atgctcaagg gccttttcgg cgatcgcttc atcatcaaag aggaagctga tctcccttga
    14581 aagaaattga gaacgaagat tatagtttac cgatccgaaa agcgaatatt tttcatcggc
    14641 aacgatcagc ttgcagtgaa gaaacgcgtg atgcggccca tcgaagagat agagcgtggc
    14701 tcctgcgtcg atcagatcga ccattccgaa gaacgcggct ttttcatagt gaaccttcac
    14761 agcgttctca ctgagcacga tattcacctt cactccccgg ttggtggctt cttttatccg
    14821 gttgatcaat tccttggtca ggaagctgta gccttgaatc atccacacct ctttttgcgc
    14881 tcccaggatg agggcatcga agagatcggt gacggaattg tcagcgaaaa aataatggtc
    14941 gctcagatag acggttttcg gcagattctc tttcggaggg acggaaaacg cagcgcggtc
    15001 gatcggttgg acactgtagc gctcccatgt ccgaacgaag gaatcgacga gcgcctcgat
    15061 cgcttgcggg ctttggatct ccaccatcgt atcgatgttc cccgtttccg ggggaatcat
    15121 caacgatgct tcattgaggt tgatgccgcc gacggccaga tactcgccat caaaaatcca
    15181 gatcttccga tggtcccggt cgaggagcag cggaaggaac gccgcatggc ttacgctcat
    15241 cggattgtac tcggcaacct tcaaaccgat atcccgggca tagttgaacg cagcgggaac
    15301 cactaggctg gtatgcggag tcaattgaaa ataggaggag gagtcaagca gcacgtacac
    15361 atcaacccct tctttcacct tctcggccaa cgcatcccat acttcgtagc ttacttcatg
    15421 aatgttgcca agaaaaaccg taaccaagat gtactctttc gcatcggcga tccgtttcag
    15481 ggtgtggaca ttccactccc ggccggtatt gaagagggtg atgtcacttg aaggataggc
    15541 aggcagaccg cgatcaacaa ggtacgcaat cgggtcgggt atgatttccc gggctcgttt
    15601 ctcaaaggtc ttcacgctgg tgcacgagat cagcgcgaag gtcattgcca agactatgag
    15661 agcggtcgtt ctcctcatat gtagcgacta taccatatct ctcttgcatg gtgacaagag
    15721 ttctactcgt gaaagactcc cagttcatcc aattgcgccc ggacgattcc cgcccaccct
    15781 tgattagcct tgggattgat cgggctcggg tgcaggatcg agccgacggt aatatcgtca
    15841 aacgcttggg cgacggtaat cagtttattc tcggcatact tccccacccc gagagcaaag
    15901 gcgggctgta acattgtgag catatcatgc aggtagctgt cgcagatcgc ctcaagagcc
    15961 atccgctccc ccagaccgag tcgatcgggg gtgatgttct tgccggtact cccccgatcc
    16021 acgaagacca gtggacagta gttgatgacg gcgatcgagc cgacgaggtc ttgggcattg
    16081 gggaaatact cctgaatcaa tccccaaagc cgttgcccgc ttacttccct gcgctgagtc
    16141 gccaacccga gaaccgggcg cttggggtgt tcaaccagcg gtcttccgat ctcctcttcg
    16201 atctgcaagt attccttcac gacggttatt tcgccgaacg gtatccccgt ctgggccatc
    16261 ccgaagggcc cggggttcat tcccagaaaa accgttttca ccggctggga aagatacctt
    16321 cggatatacg tttccatcat cggccaggca taatccaagg ggttgtaaat ataacaatca
    16381 caattgaagc gcaatgaatc cacctgattg gcgaaatctc cggttcgact gacaatcgca
    16441 ttactgaaca aattcacatc cccttcctcc accccttgcc ataaaatact cttcaacctt
    16501 ggcataaaac gcttcaaagc tcaaggcggc ccgttttgcc ctaaggagac atcgaccgtt
    16561 tgagctctag tacttttcta ctatcgctcg atggaagggc ttggattctt gacaaatata
    16621 cagactcttg tacactcaaa ctcagacaat cactagtagt tttatactag tggagatgca
    16681 ggagctcatc atgaaccatt cctgggatgt tctggaccaa tatcgcggcg gtctctttga
    16741 agggacgtgg cctgccattc tcgacttgct ctctatcacg aaagagaagt ttcccaaacg
    16801 caacgggttt accgtgttca accccgaacg cacgacgctc acctgggagg aggtgtggtc
    16861 ggaggtccgg cgcctcggct cgtatttgat tgaagcgggg atcaaggcgg atgacaaggt
    16921 ggtcatcaac ggaaagaact cccccgcctg ggcgctctcc tacttcgccg ccctctgggc
    16981 cggggcggtg gtcgtccctc tggacaatca gatgccgatc gaccgcgtcc atgcccttgg
    17041 cgagttcgtc gaagcatcct ttgtcttcgc ggatgcggca atcatcaacc agctgaataa
    17101 agaagcgcct tgggtaaaga acctgaaagg aatcgtctcc cttaccaatc ccaccgccgc
    17161 tgccgtcaac tacacaactc ttcggccgaa gaagatggtt gagcgggtca aacgcacgga
    17221 acacgacttg gcggcgatcc tcttcaccag cggaacgacc ggcaatgaaa aaggagcaat
    17281 gctcacccac ggcaacctcg tcagcgatgt gtttcaggca tgcgacggaa tctttcttca
    17341 gatcagtgaa gtggatgtcc tgtatgcgct gcttcccctt caccactcct acagctgcac
    17401 cgcagtcctg ctcgagtcga tcacccatgg gtcggagtgc gtcttcgggc acgatatcgt
    17461 cgtcagcaag atgatcaacg atatgaagag gggcaagatt accgtcttca tgggaattcc
    17521 tcttctctac aacaagatcc ttgccggaat gatgaagcag gtaagaaaga aaggagttgc
    17581 gacctacgcc ctcatccgga cgatgatgaa ggtcaacggg ttcttcaaga agcatctcaa
    17641 atggaatccg ctgcgcaagt cgtttgacaa gcttctgctc tccaagctcg gactggatca
    17701 caaccggatc tgcatctgcg gagcgggccc gctttctccg aaggtgttta cccaatacca
    17761 gcagcttgga atcgacttca tccaaggcta cgggctcacc gaaaccgctc cgattctcac
    17821 cctcaacccc gtctcccgct tcaaagtcga atcggtcgga ttggtctttc ccctcgttga
    17881 tatgatcatc gccgacaagg acgattcggg ggtcggcgaa gtccgggtaa agggaccgaa
    17941 cgtaacctcc ggatactaca aggatcccga acataccgcc gaactcttcg atgaagaggg
    18001 gtatctgaag acgggcgact tgggctatct ggacaaggag aactacctct acctcaaagg
    18061 cagagtgaag aacgtgatcg ttaccgaggg aggaaaaaat gtgtatcccg aggagatcga
    18121 ggacctcttc cagctgtacg atcaggttga gcagattctc atccgcgggt atcaggagga
    18181 aaaggatatt cccagcgaac tgatcgaagc ggtgatctac ccctcgcagg aatactttga
    18241 aggaaaagag ggcgaggtcc aaaaggagct tgatcgggtg attacggagg tgaatcaaca
    18301 gctcgcggcg tacaaaaaaa tcacccgcct gacgatcatc gacaaaccga tggatatgac
    18361 gagcaccaaa aagatcaaac ggaacaaggt gactgtttag caaaaccatg ctaggatgac
    18421 agcatgaaaa cgctcattgc caacgccctc atcatcccga tggatcgtga ggggcatttc
    18481 gttgttggag atatcggtct tgacggcccc gccatcgcct tcgttggaaa caaagatccg
    18541 tcatttaccg ccgatcgagt catcgatgcc caaggatata tcgccctgcc ctccctgtat
    18601 aacagccaca cccatctggc gatggggctg atgcgcaact acaaggacaa tgaaccgacc
    18661 cttcaggcgt ggctgaatga gatctttccg atcgaaaaca agctcgtccc cgaagatatc
    18721 ctcgtttcca gccggatcgg catcatcgag gcgctgcaaa gcgggaccgg gttcttccac
    18781 gatatgtact tcaaagccga agcgaccgcc caagcggtca aggaaagcgg aatgcgggct
    18841 aaaccgggac ttacgctgtt cggtgatctg gcagactcga aagagcgcct caagagatgg
    18901 gatgccaagg ttctgccgtt gatcgatgat ccgatcacat actcgatcgc tccgcatgca
    18961 gtgtacacgt gttcgaaaga gacgctgcgc ttcgcccatg acgcggcaaa agaacggggc
    19021 gttcctcttc acatccacct cagtgaaacg gagaaggaaa acagcgattg cctctttgag
    19081 cacaggaaaa cgcctcttca atacctcatc gacctgaact tctttgatga tgttttcgcg
    19141 ttcctcgccc actgcgtcca cctgtccgag gaggatcgga agctcctcaa ggacctccct
    19201 gccgcggtcg tccacaaccc ttccagcaat ctcaagcttg ctagcgggat cgctccggtc
    19261 gcaacgtatc tggaacaagg cattcccgtc ctcttgggga cggatggcgc ctcgagcaac
    19321 aacaacctca acctgatcga ggagatgcac cttgcagcac tgttgggccg gctttcctca
    19381 aaggacggac tgccactgaa gccctatcag gttctcgaga tggccacatc aagcgccgcc
    19441 aaggcgttcg gaatcaaggg gggaatgctt caagcgggct ttgaaagcga cctgattctc
    19501 atcaatacca agaaagccca cctcgccccc ctgaacgatc ccttcagtgc cttggtctac
    19561 agcgttcagg gaagcgatgt cgaatatctc ttcagccgag gtgagctgct gctggaaaaa
    19621 ggacgaccga cccggttcga tctgccctct tcaatcgagg cgatcaacga cgcctgggcg
    19681 gatatcaagc aacgataagg agtaccattc atggatgaac gatttgaagc cctgctcttt
    19741 gacgctgaaa cgctgattct tctcgaccaa cgggttcttc cgaccgagga aacctaccac
    19801 cattgccgca cgaccgccga ggtggagttc gccatcaggg acatggttgt ccggggagcc
    19861 ccggcgatcg gagccgcggc agcgtatggc gtctactttg ctgccaagga ggcaaagggc
    19921 gaaaaagagt tcaatgagct ctgctcgttt ctgggcaacg cccgccccac cgcggtcaat
    19981 ctcgagtggg cgatcaggcg gatgcaggct atctatcaga cgaacaagac aaggaacgat
    20041 ctgcttggac tccttctgga ggaagcccat gagatccgca gggaagacat cgccaccaac
    20101 aaggcgatgg cgaagatcgg aaacgcggtc gttcctcacg gagcgacgat cctcacccac
    20161 tgcaataccg gagcgctcgc caccgccgga tggggaaccg ccctcggagt gatcaaggaa
    20221 gcgttctacg ccggcaagga gatcttcgtc tatgcggatg aaacccgccc ccgcttccag
    20281 ggagcccgct tgaccgcttg ggagctgatg gaagcgaaga tcccttccaa gctcatcgcc
    20341 gactccgcgg cggcgaccct gatccgcgac ggaaagatcg atctggtcat cctcggcggc
    20401 gaccgggtcg cggcgaacgg ggatgtggcg aacaagctcg gaacgttcat gctcagcgtc
    20461 atctgtaaag catatggagt gcccttctat tccgtcgttc ccgtctccac gatcgatttc
    20521 gaccttgaaa gcggcgagtt gattcccatc gaggaacgag acccttcgga ggttacccac
    20581 gtcggcgaca cccaagtcgc tccgacgggc atggatgtct acaatcccgc cttcgatgtg
    20641 acccctcatc agaacatcac cgggatcatc accgaacggg gaatcatcta tccgccgttc
    20701 aaagagaata tcgcccgcct gaaacgggga gaacatcttt aagttgtata aggatatacc
    20761 tatagggtat gataagaagc aaggagatac gcaatgaaaa aagaagtagt gttttatcga
    20821 tgcaaacgtt gcggcaacgt ggcgcgcaag gactatgata tgggcccgcc gctcgtatgc
    20881 tgtggtgaag agatggtcgc gctgaaggcg aacactcagg atgccgccca ggagaagcac
    20941 gtccccgtcg taaaggtcga gggaaatcgg ttgacggtcg atgtcggcag cgtcgagcac
    21001 ccgatggtcg ccgagcacca tatccccttc atctacgtcg aaacgaagaa cggcggtctt
    21061 cgggccgatc tccaggtcgg ttccaagccc catgcgacgt tcacgatcga tgcgaacgac
    21121 aaggcggtcg cagtgtatga gtactgcaac ctccatgggc tttggaagtt cgtgctttaa
    21181 aaccgggtca acaatccgaa gagcttggct tcaagagcca tcaaaacagg atcatgaata
    21241 tccctcgcag tgcgggggat ttttatctgt tcatccatcc gcggggtgtg tggatcgcca
    21301 aggcaaagaa cccggtcacc tatgagaacc gcctccttgg tgtcatgggt gacgaagatc
    21361 gtggttcgct tctcctcctc ccatagggta agaaacgctt tgatgaggtg catcctgagg
    21421 cggatgtcca agctctggaa gggttcatcc aacagcatca gctccgcagg atgggcgaac
    21481 gcccgggcga ttgcgacccg ttggcgcatc ccaccgctga gttcatgggg atagaggtga
    21541 aggctttcct tcatctcggc aagttcaagg tagtgaagag cgatccgggt ccgctcctcc
    21601 tgttcataga acgcccggag cgttatctcg acattcgccc agacgttcac atggggaagc
    21661 agcctcggtt cctgaaagag gaagctcacc ttcccctcat ctttttccac cgttccgccg
    21721 tccggttcga caagaccggc ggccaaccgc aaaagcgtcg tctttccgcc gcccgagggg
    21781 ccgacgacgc tcatgatcgt tttcggatcg agagcgaagg agaagcgatc aaagacgaca
    21841 tgatcgccat acgccttaga aacgtttgag aagcgcatcg accctccttc gctggacggt
    21901 tgaacgagtc ttggccacta cggacacgac gccctcgaag aacagatctc ccagagcagt
    21961 gagcgcgatc gccaccagcg tccaggagag caccacgtcg gtctgaagct ggacttgggc
    22021 aagctgcatc cgcgaaccga ccccatggcg gggcacggtg agcacttcgg cagcgatgac
    22081 caccttccag atcatcgaca aggtcgcccg gcttccggcg acgaaccacg gggcgaggga
    22141 tggcagagtg tagtagaaga gcttcgtctt cggcgggaca tcatacaggt cgcacatctc
    22201 cccgaggttg ggatccaact gcttcacgcc gtgttcgatc tggaggtaca tcacggggaa
    22261 gcccatcaga aatgcggaaa agagcggcac gctcccactg gagaaccaga tgaacgcaag
    22321 caggattacg ctcatcaccg gcgtcgcctt gagggtggta acaaatggtt taagaatcag
    22381 cgaaaccgtt tgattgcgtc ctccgatcag gccgagaacc gtgccggtga tgacgatgat
    22441 gagaaaagag cggatcgccc ggagaatcgt ggccaacagg tttgcggaaa aagcggggcc
    22501 cgaggccaat tcacccagcg cacgaacgat cggcccgagc gagggcatga tcacggggat
    22561 atccacactc tttgcaatcc cctgccacag caggagaaga ccaagcgaag agaagagaag
    22621 aatcagattt cgcctggtcc tgttcatcaa atcacctaca gatacagttc gcttcccggt
    22681 acgctgccgc cgatcgaagc gggatcgagg ctatacagct gctcgaagta gaacgttacc
    22741 gcactcttcg cttcgcttgc cgggacatac accaggttgc agtgggggat cgcgctttga
    22801 gcaagcgctt tggccatgat tccaagctct tcgattttcc gggcggccaa gtcgggattg
    22861 ccgttcaccc agttcaccga atccttgtag ccgctcagca cttttctgaa gctcgcagga
    22921 taggtcgagg cgaacttttc gctgaccacg aggacgctca tcggataggt cggactcccg
    22981 gtcaattcgg cgtacagctc ttggacatcc aacagttgag tggcttggac gcttcgattg
    23041 agcaccatcg aaacaaaggg ttgcggcagg agggcgtagt cgaccttgcc ggcgatcaga
    23101 agctgggcca gctgagcggg ggccgccacc gagtaatcga aaaagatatc ctttccttca
    23161 acaagacccg cccatgcaag cagcatccgc gccatctggt cgggggtgga gcccgctccg
    23221 ggcagatgga gcgtcgctcc atacaggtcc tcgatatccg taccgccctt cgaagagatc
    23281 agggaaagca tcccgttgcc gatgaccgcg gcgagacgga tttcgacccc tttgttgtag
    23341 aggttcgcag cgacgttcgt cggaagagcc gccatgtcca gttcgccgtt ggccaagcgt
    23401 gcgatcacct cgttcggcga gggaaacacc tgaagctcga catccggctg atccagtccg
    23461 gccgacgaga atccggtggg gccggccatc acgccaaggc ggatcgttcc cgggtcggct
    23521 tctcggatcg gctggccgaa gaggccgacg acgatcacca atatcgcaat aagcatgaga
    23581 acatactttt tcatatctcg ctccttctca tcgttcgacg atgactctga agtaccgttt
    23641 ctttccgctc cgcagaatca gctcgtcgtg ctcgtcgagg aacgacgggt cgatgaccgc
    23701 ccgcggatcg tcgacttttt gatccccgat ccacgctccc ccgccctgga cgaggcggcg
    23761 ggcttcgctg ttcgttgaag caaggtcggt tcgggcaaag agctcaagca ctccgattcc
    23821 cgcttccagt tcactcatgg cgatggtgat cgaaggcatc ccatcccggt tctcgagcgt
    23881 gtcggccttg gagccgaaga acgccaacgc cgcggtgcgg gctttattcg cttcttcttc
    23941 cccgtgaatg atcttcgtct gctcatacgc aaggcgctct ttcgcatcgt tgatgttcac
    24001 gccctctttc tcgtaggtcg caatctcttc cagagagagg aaggtgaaga gcttgaggaa
    24061 cctgatcgta tcctcgtcat tgacgtttcg ccagtactga tagaagtcat agggactgta
    24121 gagctcggga tccaggaaga ccgccccctt ttcactcttg cccatcttgt gtccgtccgc
    24181 tcgagtgatc aggttgaagg tgaggccgaa gcattccggc ccgcccatct tgcggatcag
    24241 ctccactccg ctgacgatat tcccccactg gtcatccccg cccacctgga ggcggcaatc
    24301 atgatccttg ttgaggatgt agaagtcata ggattgaagg agctggtagt tgaactcgat
    24361 gaaggaaagt ccccgctcca tccgctgctt gtacgcttca aaggagagca tccggttgac
    24421 cgagaagtgg cggccgatct ccctgagaaa atcgatgtag ttgagctcgg tcagccaatc
    24481 ggcgttgttg agcatcatcg ccctgcccat tccttccccc ggatgttcgg agaaatcgac
    24541 gacggtctga agctgggatt tcatcgattc gctattggag aggatctctt ccttgctgag
    24601 catcttgcgc atttcgctct tgccggaggg atccccgatc atggtggttc ccccgccgac
    24661 gagcgcgatc ggctgatgcc ccgctttctg aaggtggtgg agggcgaaga agggaaccat
    24721 gtggccgatg tgcaggcttt tgccggtcgc atccaccccg acgtacatcc gaatcgattc
    24781 cttgtccatc aagtcgctca acccttccag attggtgcac gctttgataa atccccgatc
    24841 gaagagggtt tgcagggcac tgttcatctt acttctccac tcgcttgtag ttcttgttcg
    24901 cttccttgat cgccgaaacg atctcatcca ttccaatccg cacctgctcc atcgaatcgc
    24961 gatagcgcag cgtcaccgtc ttgtcatcca gactctgata atccacggtg atacaatagg
    25021 gggttccgat ctcatccatc cggcgatagc ggcgcccgat cgctccgctg agatcgaaga
    25081 aggtggtgaa ctcttcgcgg agggagagct ccagctcttt cgcgacttcg gcgatcccgt
    25141 ccttcttcac caagggaagc acggcgaccg tcaccggagc gacattcggg tggaagcgca
    25201 gaaccgtccg cgtttcaccg tcctcaaggg tttcctcctc atatgcgtcg ctgagaacca
    25261 tgagcacgtt acgggtcaga ccggcgctgg tctccaccac gtagggaatg tagcgttcgt
    25321 tggtcactgg gtcgaggtac gtctggtctt ttccactgaa ctcctgatgc tgggaaaggt
    25381 catagtcggt tcggttgtgc accccttcca gctcctgcca ccccatcggg aagaggtact
    25441 ggatatcgta cgcgtccttg gcgtagaacg ccagctcgtc ctttccgtgc tggtgccagc
    25501 ggaggtggct cttccggatt cccagcctct cgtagaacgc gaaccgctgg gctttccaat
    25561 actcgaacca ctcatcgtcc gtaccggggg cgcagaacca ctgcatctcc atctgttcaa
    25621 actcgacgga gcggaagatg aagtgcctag tggtgatttc gttgcggaac gcttttccca
    25681 cctgggcgat tccgaagggg atcttcaccc gggaggatga gacgacgttt ttgaattcgg
    25741 tgtagattcc ctgggcggtt tccgggcgca ggtaaatcag ggagccggca tcgctggctg
    25801 ctccgatatg ggtggagaac atcaggttga agttccgcgg ttcggtgaag gaatccttgg
    25861 ttccacacac cggacacgcc gccgcaagat cgatctgatc ggttcggaag cggcttttac
    25921 actgtttgca atcgaccatc gggtcgctga agttcgccac gtggccgctt gcttcccaga
    25981 cgcgggggtg catgaggatc gatgcgtcca gcccgacgat gtcctcgtgg atctgggtca
    26041 tctccttcca ccacagatcg cggatattgt ttttcagctg cactccgagc ggtccgtaat
    26101 cgtaggcgcc gttcaagccg ccgtagattt cgctcgattg gaagacaaat ccccgccgct
    26161 tgctgagtga tacgattttc tctaacgtaa cttctgccat cttattcaat tcctttcttc
    26221 agcacgttca gtgcgtcttg tattcggttc atcgtgtggt caaagccgag cagctcaatc
    26281 gagtcgaaga ggggcagact gaccgtgctg ccggtgatcg cgacccgaag aggctgaaac
    26341 accccgttga ccttcatccc gagatccttc gccaattgga cgagctcctc ttcgatcacg
    26401 ctttgctctt tctttgcttc caagccgcta aggagcactg tataagcacg ctcaagggcg
    26461 gtatgggcca acggaagctc gacgcccttc gcggcgaaga ttgaaaggtc ctcccacacg
    26521 ggctcttcaa agagaaaacg agccaggccg gtgatgtcgc cgaggacttt cagccgctct
    26581 ttcgcgaccg cggtcagatg cagcagcttc gctcggtcgg tcaagggcca ccccgtctcc
    26641 gccagatacg gcatcaacag atccaagagc tcttcatcgc tcttcatgcg gatgtattgg
    26701 ccgttgaacc agtcgagctt cttgtagtcg aagactccgg gagccttgtt gatcttctcc
    26761 aggctgaaga gctgttccag ctcctccttc gtgaagaact cccgggttcc gtcatacgac
    26821 catccgacga gggacacgta gttcaggagc gcttcgggaa gatagccttc ttttctgaac
    26881 tccgccaccg cggtcgaacc gtggcgcttg gagagcttct gcccgtcctt gcccatcacc
    26941 atcggcaggt ggcagtactt cggcggctcc cagccgaacg cttcatagag gaggatgtgc
    27001 aagggtccgg agggaatcca ctcctgagcg cgcatgatgt gggtgatctc catcatgtgg
    27061 tcgtcgatga cgttcgcaag gtggtaggtc ggaaacccgt cgctctttaa cagcaccgga
    27121 tcgggggaca catcgctgtt cctgcgggtg atcgtgccca tcaggatgtc ttcaaagctc
    27181 gtctttccct cggtggggac tttgaggcgg atgaccggcc tgatcccctg ttcttcatac
    27241 gccaagcgct cttcactgct taagttcctg cagtgacgat catacccctg atattggctc
    27301 ttggcctcct gctgctcttt cctgacccgt tccaagcgct cggggctgca gtagcagtag
    27361 tatgccttgc cgtcttcaac gagctttttg gcatacattt catagatgtc gaagcgctcg
    27421 ctctgaacat acggagcaaa gggtccgcct ttgatcggtc cttcatcata ctcgatgccg
    27481 agccactcca aggtggcgta gaggtcatcc agcgcttcgg tcgaatagcg ctcctgatcg
    27541 gtatcctcaa tccgcaaaat gaacgagcct ttttgtgagc gggcaaagaa ataattgaac
    27601 aacgctgtcc tgattccacc gatatgctgc aatccggtcg gagaaggcgc gtaccgcacg
    27661 cgtacttgca tgaacatgac tcctcttatt tcctttgttt taccgcaaat atgaaataat
    27721 gacaacagag gacagaggat ccc
//
LOCUS       DAOSLI010000023        26701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2013_length_26701_cov_24.857727, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000023 DAOSLI010000000
VERSION     DAOSLI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 26701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26701
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2013_length_26701_cov_24.857727"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLF67_04840"
     CDS             <1..506
                     /locus_tag="PLF67_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89376.1"
                     /translation="DFLHTLALSLGIEQKRGITSIALGLGGEVDLLHKPAGITMQPSI
                     FVHITPKLLFPIGKTSFVITPELVGRFVFGKDPAYEVEARVAIGFRFGSKPKTAVPPP
                     PAVLAASAPLVPPLPTIRSAVAIREEVAPQPVVVEEPIKQPEQPAVDPEVRKEQIRRL
                     WWLLGRP"
     gene            complement(503..1561)
                     /locus_tag="PLF67_04845"
     CDS             complement(503..1561)
                     /locus_tag="PLF67_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89377.1"
                     /translation="MKKTMRISVLLCVLVCFSAMGLGAFSFELNPQTPLHLPYLADPF
                     NTDLALGYMYLSSEEDRPAYLYKVTSGSSGVEYTGLEINKYYTDGTNMIMMRTGSSVP
                     LARISVGPLAVEANIRGGFRAFFFAYRGTDLLGFDGTYYLGMNAKLGNLLTFSFGKKH
                     HSGHIGDEIIATIDKKDPVDGSLLHDRQIDYVRQDPLTFALSFNALNHLRVYGELRLG
                     DTAQIFKPKFISDEAVADGYRARELEVGAELSLPIPYVGDFTVAFDMTLHEMGKFVPQ
                     GSGTVINGEYEKYTFTYEKDAPWETEFQIVLAQSLTTREEGLKARVMVTYHRGRFPLF
                     AFHMSKGSFISVGGSMGF"
     gene            complement(1732..2601)
                     /locus_tag="PLF67_04850"
     CDS             complement(1732..2601)
                     /locus_tag="PLF67_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE89378.1"
                     /translation="MKKDSQYLKGILITFVGVLVLSSDALLIRMSSVPGFRAAFWRGL
                     FTFLSVSIIFLITNKKEAATIVKTQYKRMLFPALLWGSSGVAFAIGVHNSGASIALVM
                     ISLGPFFASFQSFLFYREKPHPLTLLAALGAVGGIVYMFGSQLGTLEAKDFFYNIWSP
                     LLYGTNLAYLRNNPTLNRQGISILGGFTAMILALFISRFQIVIPFKNLLPLIVLGAIV
                     IPFAQLSIGHGTRFIPAAESALISSLETIIGIIYVWIFLNEAPSLHTILGGAIVFSCI
                     VFNTVVQAYRVKR"
     gene            complement(2603..3592)
                     /gene="trpS"
                     /locus_tag="PLF67_04855"
     CDS             complement(2603..3592)
                     /gene="trpS"
                     /locus_tag="PLF67_04855"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOE89379.1"
                     /translation="MRSRRRILTGDRTTGKLHLGHYVGSLKSRVELQDEYETFILLAD
                     IQALTTHFNEPELINSSILDVTMDNLSVGLDPEKVTFVQQSQITSIAELTVFYSMIVS
                     VNQLRHNPTIKTEAANYGYEDLSYGFLGYPVSQTADISFINADLVPVGEDQVPHIELS
                     RKIIRKFNKLYGTNITVPRAKLSEVGRLAGLDGNAKMGKSMGNAIYLSDSPEVVWQHV
                     RKAVTDTSRVHAHMEGHPEVCSVYKYHQVFNPGETDEICKGCTSAALSCFACKQRLNE
                     VLNNLLEPMRERRAYYENNIDIVKELIHEGSKKANTIGNENLERIKEKMHILI"
     gene            complement(3589..3759)
                     /locus_tag="PLF67_04860"
     CDS             complement(3589..3759)
                     /locus_tag="PLF67_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE89380.1"
                     /translation="MPSYEYRCRLCKATIELVQSVGKHQVPEVCPSCNMKHTMVRVNI
                     PVSTKEKEGEKR"
     gene            complement(3772..4635)
                     /locus_tag="PLF67_04865"
     CDS             complement(3772..4635)
                     /locus_tag="PLF67_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89381.1"
                     /translation="MKKAKIVLLALFAVMLLGSCTTTTVRKRPAFTIEELPTHRTILI
                     TTNDPGIIQAVLPDVADRSERISVVIEPEDDAYPLNLEASRIYGVMETTYSRVAMNTA
                     LMWSSKTKKDLHEDPSYFQMKESDLELYSPKNGKLLFTLDDYPAVYEQLSKEDGKRLS
                     AEIAERMKESAIALYAEYPRTFFDLNLGVSEEVVRQSESILLLFDRSEEGGYVLNAFI
                     TMGDEKKAKTLSQMVRSGYIANLKKANQKYVIAELMKMFLLDGQLVTIKGIEITEDEL
                     FKILAGLDGLI"
     gene            complement(4635..7766)
                     /gene="ileS"
                     /locus_tag="PLF67_04870"
     CDS             complement(4635..7766)
                     /gene="ileS"
                     /locus_tag="PLF67_04870"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOE89382.1"
                     /translation="MFRPVNPKVDFPKMEEGVLQFWEERNIFKKSIEYRPEEKEYVFY
                     DGPPFATGLPHFGHIVPGTIKDTIPRYQTMKGRRVNRRFGWDCHGLPVEYEIEKSEGI
                     SGYSAIVEFGVARFNEMCRSIVLRYTKEWEITIKRTGRWVDWEDSYRTMDLSYMESIW
                     WVFKTLYEKGYIYEGYNILPYSPKLASPLSNFEVNLGGYQDVKDPALTVRFKVDGEEN
                     TYFLAWTTTPWTLPSNLALAFGPQIEYVKVLDKRDGNYYILGKDRLSHYYTDEELYEV
                     VDTRKGSFYEGLHYEPLFPYFADEKEKSGAFVTVLGDYVTTEDGSGIVHTAPGFGEDD
                     YQVLKGTGIPTICPIDMECNFTEEVSDYAGRFVKDCDDDIIEYLKEHNLLFLEETIVH
                     PYPFCYRTKMPLIYRAMSSWFVDIDQIKPFMLAANEQIYWMPEHLKYGRFGKWLEGAR
                     DWSISRNRFWGNPIPVWKSDDGNHIEVIGSLEELEAKCGQKVTDLHKHFVDDLTWPSP
                     DGKSTMRRIPDVLDCWFESGSMPYAQLHYPFENQEYFEKHFPADFICEGLDQTRGWFY
                     TLTVLAAGLYEKPAFYNNITNGIVLTAEGKKMSKSERNYTDPMELINQYGADALRFAL
                     MSSAVVRAEDLKFSEEAVKDVLKGLLLPLWNAYSFFVTYANIDSYEPSATNHADLKNP
                     MDQWIVSATARLVENATAAFDAYDIQKACSYFIPFLDELNNWYIRRSRRRFWRGENDE
                     DKKQAYDTLYHALMTFIKVAAPVIPFTVEEIYQNLRSNELPESIHLCDYPVYREEERN
                     FILEEQMALTQKAIALGRALRAAHTLKIRQPLSKLFLVDRNEHERKVLASMQESIAEE
                     LNVKSVSIEADETALVTYSAKANFKLLGGKLGKDMKEVAALIQELDSKTIARILDGGV
                     YPLHYSNGTIDLGVEEIIVQRSEIEGVKVINDGTLTVGFDTAMTEELLDEGLMRDLIR
                     AIQNLRKESGFDVTDRIMIAYEGDDKIHDVFTKFESIISDETLADEITSKPLDGEALQ
                     LQEYQVRLELKKV"
     gene            7885..9246
                     /locus_tag="PLF67_04875"
     CDS             7885..9246
                     /locus_tag="PLF67_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE89383.1"
                     /translation="MRTFPITGRIKRFASVFRDNGYSLFVVGGAVRDFLLGLGNSDLD
                     FTTDARPEEVIALFRTVIPTGIAHGTVTVIFEGEHYEVTTFRSEGEYKDGRRPSSVTF
                     IPSLEEDLKRRDFTINAFAASAITGKIVDNHNGEQDLKDGIIRAIGNPYERFDEDALR
                     IMRAARISGKLNFTIEDQTFNAMGALKENLRLVSVERLHDELVHLVKSDHPRKGFEYL
                     HASGALAVILPELAQGDGVLQKGFHHEDVLQHSLTTCQKAADLGASVYVRLAALFHDI
                     GKATTREPNGDRYSFYNHEIVGEALTKTILRRLKASNEEIDTVSHLVRHHMFSYTPEM
                     TDSAVRRFIVRVGIEHIEDLFKLRIADQMAISGRADTDLLDEFSKRINGIISSDDALS
                     IKDLAIGGNDLLALGIPKGKRIGQTLQYLLETVLDDPAQNTKEQLFRIALAYQELCGF
                     TSD"
     gene            complement(9216..9962)
                     /gene="rnc"
                     /locus_tag="PLF67_04880"
     CDS             complement(9216..9962)
                     /gene="rnc"
                     /locus_tag="PLF67_04880"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOE89384.1"
                     /translation="MDELRTKEAPSLAPERERELLAFNEASGIGIDDLALLDLAFTHR
                     SHANETGDRTRNNERLEFLGDSILGLAVADLLLEAYPDKEEGELSKIKSIVVSEDSLA
                     LAAKKLNVAQYLLLGKGEEQTGGRRKKALLADCTEALFGACYMHNGFPAAKAFVSRAL
                     GERITAVVEDDYHRDYKTALQEYMQKKWRIVPTYRLVSKTGPDHNFTFYVEVDVNGQV
                     FGPAHGRSKKAAEQAVAKIAYQSLVNPQSS"
     gene            complement(9969..10208)
                     /gene="acpP"
                     /locus_tag="PLF67_04885"
     CDS             complement(9969..10208)
                     /gene="acpP"
                     /locus_tag="PLF67_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOE89385.1"
                     /translation="MDNTQVFEKVKALIAEKLEIEESKITMEASFRKDLGADSLDTYE
                     LVYAIEEELGISIPDEKANEFETVKDAVEFLASEL"
     gene            complement(10227..10412)
                     /gene="rpmF"
                     /locus_tag="PLF67_04890"
     CDS             complement(10227..10412)
                     /gene="rpmF"
                     /locus_tag="PLF67_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOE89386.1"
                     /translation="MATPKYKTSKARSASRKSANMKLAAPTLSRCGTCGNMVVPHRVC
                     PKCGFYRGVQVIELQDK"
     gene            complement(10628..11128)
                     /gene="coaD"
                     /locus_tag="PLF67_04895"
     CDS             complement(10628..11128)
                     /gene="coaD"
                     /locus_tag="PLF67_04895"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOE89387.1"
                     /translation="MNSNIRTAMLPGSFDPPTLGHVDIIERSARLYDRLYVVIADNVH
                     KSYLFTPEERLAMLREILREHENIEVVAHSGLVVDFAKTHKVGVMVRGVRALADFGYE
                     FELAMTNKQLYPGLEVLFLPTSPKYFLLRSSAIKEMAAFGADISTMVPPVVAQIMKQR
                     LKAAKE"
     gene            complement(11125..11574)
                     /locus_tag="PLF67_04900"
     CDS             complement(11125..11574)
                     /locus_tag="PLF67_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89388.1"
                     /translation="MDSQVKKPKKDRRRGNRRNRKAKGSQKKVEQEIFVQPSEKKFTP
                     TLPPLPVKKVVEPKEVCVMCKKPITLIANALTHPQGGYCHFDCVLGFLTEREQLKENQ
                     TISYIGRGMFAVAEKNSEGSYTFVKRIEWESPESFAAMKKYVEEMKA"
     gene            complement(11665..12021)
                     /gene="rplS"
                     /locus_tag="PLF67_04905"
     CDS             complement(11665..12021)
                     /gene="rplS"
                     /locus_tag="PLF67_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOE89389.1"
                     /translation="MDVMKAVESVQLKENAENFSVGDTVKVHFKIVEGATERIQVFEG
                     LVIAKNNSGVRRTFTVRKISYGVGVERIFPLHSPRVERVEVVRLGRVRRAKLYYIRQR
                     VGKKAKVKELIRKKNA"
     gene            complement(11999..12760)
                     /gene="trmD"
                     /locus_tag="PLF67_04910"
     CDS             complement(11999..12760)
                     /gene="trmD"
                     /locus_tag="PLF67_04910"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOE89390.1"
                     /translation="MTITILTLFPHMLEGFFTSSIMRRAVEKGTIAYKLIDFRSWATD
                     KHKSVDDLPYGGGAGMVLMYDPLSKALDELGTRGKRVVYPSPSGVLLNQKLAHELSEE
                     RELIFICGHYEGVDQRIIDEYVDDEISIGDYVITSGEVATMVIVDSVFRLIDGVISRK
                     SLDEESFSDGLLEYPQYTRSEPSCPKEVPHVLLSGHHEKIESWRLKQKLLRTMERRID
                     LLETASLDAVSRKQVNELKEHMAKGIVDNGRDESC"
     gene            complement(12757..13263)
                     /gene="rimM"
                     /locus_tag="PLF67_04915"
     CDS             complement(12757..13263)
                     /gene="rimM"
                     /locus_tag="PLF67_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOE89391.1"
                     /translation="MDRLIATATIGRTFGYEGEVRVYPNNEESDYLTKLKTIVASLKD
                     GKEATLAVEYVRADGKSVLMKFKGYDSSEEAQTLVGARVMVDRAFASPLKEGEYYTAD
                     LIGCTLVSGGEALAMVVSVLDGPQALLLEAERPDSKRFLVPFLYEYTGEVDLEAKTIE
                     LLSPWLLD"
     gene            complement(13265..13498)
                     /locus_tag="PLF67_04920"
     CDS             complement(13265..13498)
                     /locus_tag="PLF67_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOE89392.1"
                     /translation="MEKDLVEYIVKSLVDLPDQVDVNVVEGEKSTILELRVASEDVGK
                     VIGKQGRIAKAIRTILSASATRDGKRAVLEILD"
     gene            complement(13515..13781)
                     /gene="rpsP"
                     /locus_tag="PLF67_04925"
     CDS             complement(13515..13781)
                     /gene="rpsP"
                     /locus_tag="PLF67_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006433756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOE89393.1"
                     /translation="MVSMRLKRFGSKKRPDYRIVVIDSREKRQGRAIEEVGHYHPLAE
                     KKEEQLVIKEDRVKEWLMKGAQPSDTVRNLLNKSGVQVNRKARD"
     gene            complement(13808..15142)
                     /gene="ffh"
                     /locus_tag="PLF67_04930"
     CDS             complement(13808..15142)
                     /gene="ffh"
                     /locus_tag="PLF67_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOE89394.1"
                     /translation="MFDSISNAFSSIMRSIAGKSKISEKNIQDAVEEIKVALLDADVN
                     IRVVRRFINSTMEEALGEKVLKAVDPGDQFVKIVYDKMVNLLGDEENQKLNLKGPDTP
                     SSILMMGLQGSGKTTTAAKLALRLKKEGRRVLLVAADLVRPAAILQLQVLGKTVGVEV
                     FSIEGEKDPAKVAKAAFAKAKKEQFNTLIVDTSGRMHLDDVLMAEIKKVRDALKPEET
                     LFVADAMTGQQAVTIAKEFEEQVGISGVILTKFDSDTRGGAALSLRSVVGKPIKFIGV
                     GEKVEDLEPFYADRYASRILGMGDIVSLVEKAQEVFDEEQAIKMQQKMAKNTFDLQDY
                     LEQLESMNKMGGLESMLELIPGMKGQVSEDDIDKQEIEREKAIIRSMTYAERENYLIL
                     GPTRRRRIAKGSGTHISDVNRLIKKFEKSRLMMRKLTRNKKYQAEMLKQMGL"
     gene            complement(15208..18054)
                     /locus_tag="PLF67_04935"
     CDS             complement(15208..18054)
                     /locus_tag="PLF67_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE89395.1"
                     /translation="MFLKSLELYGFKSFADRVKLQFSEGITSLLGPNGCGKSNIVDAI
                     KWVLGEQSTKTLRASKMEDVIFNGTDLRKPLQVAEVILVLSNENGDLPIDEPEVEIKR
                     RIFRTGESEYYINRNRARLMDIRDLFFDTGVGKSAYSILEQGKIDQILSSRPEDRRYI
                     FEEAAGISRYKVQSLEAKRKLERTDENIAQVETILREVKRNYDTKKGQAEKAKNYREL
                     RKEQFTLEVDIQLSTLVSYSKLREVRLEQERTFSRHYQAKRAELETFDENLANLREEL
                     RTLGASRIAIQTELQRLDEATKGRGEKLDLLTVRYRDFLNQQESAKSRSEGIQGQINR
                     DKAHIAERTEHITQIDEQIRQLEGELEGFLASLGKNRKTIEDHINQINKLEKSNARLE
                     EETDTLSLEVKELSDAIVIQLEEQLRSSGYTTKARNEARSILLSELEHIKQSILKEQE
                     FLAKLKAADLSVETLFKRQHEFFSTLGDSLASLSGHFTAYEGTQASFLDELIAPEGTI
                     AKKQSLDLAIAQKRKEIIRNRERVSHLREENLHLQSQMDLFQDAITEHQSALSQLRSN
                     KESAQAWIANLRSTITEREYEYKDALAAATNARERIHETQEDIRATEAEVKEIKTRIV
                     DLNQELEELATRIEEQNLLIQNRETEKEDAFNEIQNLRSEREKAELHAQNLEEQIAGL
                     YTNFFDTYGKSLKEFEHRLEEEELDDSPVLRARLEEVKKRVSGMGYINQMAEQEFSEI
                     KEQYDFLTRQLDDLAKAKADLDSVITEITTRSEELFMATYKEISAAFQAMFRRLFGGG
                     RAELSLVDPDNVLESGIDILAQPPGKKLTHLTLLSGGERSMTAVALLFATYSVKPSPF
                     TVLDEIDAALDDRNIGYFLSLLEEFAQKSQFIIVTHNKHTVMGSTTLLGVTQMEPGVS
                     TMVSYRLDTLEGKPVILNDDEQAVDFNEEGSLIR"
     gene            18127..18678
                     /locus_tag="PLF67_04940"
     CDS             18127..18678
                     /locus_tag="PLF67_04940"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOE89396.1"
                     /translation="MTLICGVDEAGRGPLAGPVVAAAVILGKHFPAHLLKDSKKLAPK
                     QRTMLETLIKEQAAGWTVAVVSHRRIDEINILQATMEAMHNAVSQLQKNHPITKALID
                     GNRSPILSCETETIVKGDDSIPEIMAASILAKVERDRIMVAYDEQWPMYRFAEHKGYP
                     TKAHKEALKQYGPSPIHRLSFRY"
     gene            complement(18675..18893)
                     /locus_tag="PLF67_04945"
     CDS             complement(18675..18893)
                     /locus_tag="PLF67_04945"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOE89397.1"
                     /translation="MFSTRLVKNERTYFFNVKENIRGDVFLNIVESKGLPDSDRFIRQ
                     SIIIYQEDLGEFLKELQKSLDFIKQNTR"
     gene            19052..19122
                     /locus_tag="PLF67_04950"
     tRNA            19052..19122
                     /locus_tag="PLF67_04950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19084..19086,aa:Gly,seq:tcc)
     gene            19174..20343
                     /locus_tag="PLF67_04955"
     CDS             19174..20343
                     /locus_tag="PLF67_04955"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOE89398.1"
                     /translation="MQERYAELVIAIQLRLSEGDSLTINTNARTMSFARLVARKATEV
                     TKLPVTIVETSMGKVLQTYPIDPVEKGVMRPQVRTSVLAHIVDLDEFPYDEDEDWNEP
                     HHEVRTLAHYGHLSDPVFLDRRISIPWANIPYPGTAWAASMLGRPVSREEMWSLFGIL
                     MRLTSDWSPAYWAEQANVLSYREKLLNASSGRMISIKGSNTELIGEQSPLTGWISGVF
                     RLPSGREYIPLLPVQSVHTAFKTGSVNGKIRSGSPFTLFGTPVTDAAFIIENGVVVSY
                     DAREGKEALKKYFDVDAGTNAASGISLADSNTLECRYITRLVHPHYKKECRSILHLGG
                     VLVDAIKAGTGEDDLFGLGQSIAHLEIPLAHDITVEMQKPDGSMKTIIAEGSFTV"
     gene            20358..21587
                     /locus_tag="PLF67_04960"
     CDS             20358..21587
                     /locus_tag="PLF67_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOE89399.1"
                     /translation="MTEQDIRRYAELIITKGINLQKGKAVLITTGAGTYYFARALGKA
                     AYRLGASYVQILTDDLDLLSSRLEAQSEEEVQFVPAYLKALDYEFISEGWSSIRIDST
                     EERLDHGPLDPEKNRLASAARRKASEARSRVLMRHQLPWCVCVAPGPLWAKQVLGEDA
                     KPEDLFEVLKPILLLDREDPGTAWDEKHETLQKRMDFINALDFKALHFQSPRTDLTIG
                     FRKEARFVGGAETLPSGKRFFANLPTEEIFSTPDRMQVEGYVTTTRPVEVLNTPTEEV
                     RLVFKEGKVVEHSAKKGQEVLDRFFEIDEGARRIGEVALVDESSPIAQSGLIFHSILL
                     DENASCHLALGEGYPICLEHGSTLSNEEELHEAGCNTSLVHVDFMVGSSDMNVDAILS
                     DGSRVPIIVDGRFSLNH"
     gene            complement(21571..22332)
                     /locus_tag="PLF67_04965"
     CDS             complement(21571..22332)
                     /locus_tag="PLF67_04965"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOE89400.1"
                     /translation="MLIAVDIGNSHIVVAVHSGKEWIHSFRIHSDQKKTTDEYFVVLE
                     ALMNNAKISRQDITKAVISSVVPNLTRAIAKNIYNLFSIQPLMVSHSVNTNLIRETIP
                     KELGMDLLANVAQAHYVHPDRCVMVIDFGTALTFSTVNGEGAVLGVSIAPGLVTAVNS
                     LFGATAQLPQVELKTPPSVIGRTSQDSINAGIMFGYAGLVKSIIELTEAEIGEELYVI
                     ATGGLSRTLSPLVGRINQIAPMHTLDGLKLISDLN"
     gene            complement(22343..23980)
                     /locus_tag="PLF67_04970"
     CDS             complement(22343..23980)
                     /locus_tag="PLF67_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE89401.1"
                     /translation="MITASNIGLSYGTQVLFKEVDIKFTPGNCYGIIGANGAGKSTFL
                     KILSGEIEPDTGQVIITPGQRLATLRQDHFAFNEYSVLDTVIMGYEKLYSIMKERDEI
                     YAKEEFTEEDGLRAAELEGDFAELGGWEAESEAAQLLSGLGIADALHEKQMAEVEDSI
                     KVRALLAQALFGNPDILLLDEPTNHLDLESIHWLEDFLSDFDNTVIVVSHDRHFLNTV
                     CTHIADIDYGKIQLYVGNYDFWYLSSQLAAKQQRDEKRRREEKIAELKEFIYRFSSNV
                     AKARQATSRKKLIDKLTIDDIRPSSRRFPYVAFKPNREVGRNILDVDSLSKSIDGEVL
                     LDKFSLHVNAGDKIAFVGPNHAAKSALFEILSGNDKQDGGTYTWGVTTSLSYFPKNNN
                     HLFEEHVPIVDWLRRYSPDQDETYVRSFLGRMLFSGDEALKDLTVLSGGEKVRCLLAK
                     MMLEGANVLIFDEPTSHLDLEAITALNNGLIEFTGNILFNSHDHQFVESIANRIIEFT
                     PGGVIDRMMSFEEYFKDEGIKALRTELYGGVKNALAL"
     gene            complement(24038..24109)
                     /locus_tag="PLF67_04975"
     tRNA            complement(24038..24109)
                     /locus_tag="PLF67_04975"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24074..24076),aa:Glu,seq:ctc)
     gene            complement(24115..24185)
                     /locus_tag="PLF67_04980"
     tRNA            complement(24115..24185)
                     /locus_tag="PLF67_04980"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24151..24153),aa:Gln,seq:ctg)
     gene            complement(24308..24841)
                     /locus_tag="PLF67_04985"
     CDS             complement(24308..24841)
                     /locus_tag="PLF67_04985"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOE89402.1"
                     /translation="MSVNIDLDLAIRKVPHFPKEGILYYDITGILNDSNAFRASIDEL
                     AKRAGSYQFDYIAAVEARGFVFGAPLAERLGVPLILIRKKGKLPNEVYSKSFALEYGE
                     DVICIQKLDVHPGKKVLLVDDLIATGGTLEAAASIFNENGAIVTGILGVIGLPFLNYT
                     EVLKAYPTEVLITYDSE"
     gene            complement(24838..25857)
                     /gene="tsaD"
                     /locus_tag="PLF67_04990"
     CDS             complement(24838..25857)
                     /gene="tsaD"
                     /locus_tag="PLF67_04990"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOE89403.1"
                     /translation="MNVLGIETSCDECAASVVVDGKTILSNVIATQTEEHRIYYGVVP
                     ELASRIHTEWIAPTVQAAIDEAGVEIDGVAVTNRPGLLGSLLVGVSFAKGYAAAKGVP
                     LIGIDHIRAHLYAPQIEHELDYPYLGVLVSGGHTVICKVTGYDDLEVLGTTIDDAIGE
                     AFDKVAKHYDFGYPGGIAIDRLAQKGNPLAFRFPGPKIREKTNPYDISYSGLKSAVIN
                     HLDKYWDGKSEKSSANIAASFQRTAINMLIKRIRAALADTGLKSISVGGGVAANSYLR
                     SELKKFGSGGYTVAFPSMELCTDNAAMIAALGYHYLKDGIRDRYDMKASARVSAFKKQ
                     YTPGG"
     gene            complement(25854..26063)
                     /locus_tag="PLF67_04995"
     CDS             complement(25854..26063)
                     /locus_tag="PLF67_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89404.1"
                     /translation="MKKRMVFTPAAPREVIEILVSHPARFRPIVGQSWDPNVADFLIE
                     MYPEAIEQGFALEEIALMVAEDEEA"
     gene            26163..26234
                     /locus_tag="PLF67_05000"
     tRNA            26163..26234
                     /locus_tag="PLF67_05000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26195..26197,aa:Thr,seq:cgt)
     gene            complement(26408..>26701)
                     /locus_tag="PLF67_05005"
     CDS             complement(26408..>26701)
                     /locus_tag="PLF67_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89405.1"
                     /translation="SGDQAEVAIFPGFGPGFFSPGRMAPSMDKDKKIQEMIREVGLDK
                     EMTKERLKELTTYLKSLPLSDLGVESLKVAKAMSENSKGKYSIGEMYQAMIDY"
BASE COUNT         5914 a         7413 c         6771 g         6603 t
ORIGIN      
        1 cggattttct tcacaccctt gccttgagtc tggggataga gcagaagaga ggaatcacct
       61 caattgcctt gggtctcggc ggtgaagtcg atcttcttca caaacccgcc ggaattacca
      121 tgcaaccgag catcttcgtt cacatcactc cgaaactcct ttttccgatc ggaaagacct
      181 ccttcgtcat cactccggag cttgtcgggc ggttcgtgtt cgggaaagat ccggcgtatg
      241 aggttgaagc acgggtagcc atcggcttcc ggttcggctc aaagccgaaa accgccgttc
      301 ccccaccccc ggctgtctta gccgcatccg ccccgctcgt cccgccgctg ccgacgatcc
      361 gaagcgccgt ggcgatccgt gaagaggtag ctcctcaacc ggtagttgtg gaggagccga
      421 tcaagcaacc cgaacaaccg gccgttgatc cggaagttcg aaaggaacag atccggcgcc
      481 tctggtggct gcttggcagg ccctagaaac ccatcgaacc tcctacgctg ataaacgagc
      541 ccttgctcat gtggaacgca aagaggggga atcttccccg gtgataggtg accatgactc
      601 gtgccttcaa gccctcttcc cgcgtggtga gcgattgggc cagcacgatc tggaactccg
      661 tttcccaggg agcatctttt tcataggtga atgtatactt ctcatactcg ccgttgatga
      721 cggttccact gccctgggga acgaatttcc ccatttcatg caacgtcata tcaaaagcta
      781 cggtgaaatc ccctacatac ggaatgggaa gactgagctc cgctcccact tcaagctcgc
      841 gggcccggta tccgtcggcg accgcttcat cgctgatgaa cttcggctta aaaatctgtg
      901 ccgtgtctcc caacctcagc tcgccataca cacgaagatg gttcagggca ttgaatgaaa
      961 gagcgaaggt aagaggatcc tgtcgaacat aatctatctg ccggtcatgc aacagcgaac
     1021 cgtccaccgg gtcttttttg tcgatcgtgg cgatgatctc gtcgccaata tgtcctgaat
     1081 gatgcttctt tccaaaggaa aacgtcaaca ggtttcccaa ctttgcgttc atccctaaat
     1141 agtatgttcc gtcaaatccc aagagatccg tccccctgta ggcaaaaaag aacgctctga
     1201 atccccctcg gatgttggct tcaactgcta gaggtcccac actgatccgg gcgagaggca
     1261 cggaagagcc ggttctcatc atgatcatat tggtcccgtc tgtataatat ttattgatct
     1321 ccagccccgt atattcaacg ccgctgctgc cgctcgtcac cttgtacaga tacgccggac
     1381 gatcttcttc tgacgagagg tacatatatc caagagccag atccgtattg aacggatcgg
     1441 ccagataggg aagatgcaac ggtgtctgcg gattcaactc aaaagaaaat gcgccgagcc
     1501 ccattgccga gaagcatacg agcacacaca ataaaacgga aattcgcatc gtttttttca
     1561 cattgactcc ttaaacctgt caagcagaga agacgttacg agatcaacat gttcggcaat
     1621 tatcaaaatc cacaccgctt gagcaaaaac gtataaaccg cattatccca tagatgcatg
     1681 tttgccgcaa taagagatga acaaatatgt tccaaaggtt catctttctt tttaacgctt
     1741 tacccgatac gcttggacga ccgtattgaa gacgatgcag gagaagacga tcgctccccc
     1801 taagatcgtg tgaagggacg gggcttcgtt taggaagatc cagacataga taatcccgat
     1861 gatcgtttcc agactgctga tcagggcact ctccgcagcc gggatgaacc gggttccgtg
     1921 tccgatcgag agctgggcga acggaatcac gatcgccccg agaacgatga gcgggaggag
     1981 attcttgaag gggatgacga tctggaatcg ggagatgaag agtgccaata tcatcgcggt
     2041 gaatcccccc agaatactga tcccctggcg gttgagcgtc ggattattgc gcagataggc
     2101 aagattggtt ccatacagga gcggtgacca gatgttgtag aagaaatctt tcgcttcaag
     2161 ggtgccgagc tgcgagccga acatatagac gattcctcct accgctccga gggcggcaag
     2221 cagagtcaac gggtggggtt tttcccggta gaacagaaag gactgaaacg aagcgaagaa
     2281 cggaccgagg ctgatcatca ccaaagcgat cgaagcaccg ctgttgtgta ctccgatcgc
     2341 aaatgcaacg ccgctggagc cccacagcaa ggcgggaaag agcattcgct tgtactgagt
     2401 ttttacaata gttgcggcct ctttcttgtt ggtgatgaga aagatgattg acacggacaa
     2461 gaaggtaaac agtcccctcc aaaaagcggc gcgaaacccg ggcacactgc tcattcgaat
     2521 aagcaaggca tcactgctta aaacaagaac ccccacaaac gtgatgagga ttcctttgag
     2581 gtactgagaa tctttcttca tgtcagatca ggatgtgcat cttctccttg atccgctcaa
     2641 ggttctcatt gccgattgta ttcgccttct tcgatccttc atggataagt tctttgacga
     2701 tatcgatgtt gttctcatag taggcacggc gttcgcgcat cggctcaagc agattgttca
     2761 acacctcgtt gagacgttgt ttacaggcaa agcaggaaag cgctgcgctg gtgcaccctt
     2821 tgcagatctc atccgtctcc cctgggttga agacctgatg gtatttgtag acgctgcaca
     2881 cctcgggatg cccttccatg tgggcgtgaa cccgggaggt gtcggtgacc gctttgcgga
     2941 cgtgctgcca gacgacctca ggggaatcgg aaaggtagat cgcattgccc atcgacttgc
     3001 ccatcttcgc attgccgtca aggcccgcca agcgcccgac ttcactgagc tttgcccggg
     3061 gaacggtgat attcgtaccg tagagcttgt tgaacttccg gatgatcttt cgggaaagct
     3121 cgatatgggg gacctgatcc tctccgacgg gaaccagatc cgcattgatg aaggagatgt
     3181 ccgcagtctg gctgaccgga tatcccaaga acccatagga gagatcctcg tagccgtagt
     3241 tggccgcttc cgtcttgatc gtaggattgt gccgcagctg attgacggag acgatcatcg
     3301 agtagaagac ggtcagctcg gcgatcgagg tgatttggga ctgttgaaca aatgttacct
     3361 tttccggatc gagccctact gagaggttgt ccatcgtgac atcaaggatt gatgaattaa
     3421 tcaattcagg ttcgttaaag tgggtggtga gcgcctgaat atcggcaagg aggatgaacg
     3481 tctcgtactc atcctgcagt tccacccggc tcttcaaaga accgacatag tgaccgaggt
     3541 gcagctttcc cgtggttcga tccccggtga gaattcgtct tcggcttctc atctcttctc
     3601 cccctctttc tccttggttg agacagggat attcacccgg accatcgtgt gcttcatatt
     3661 gcaggaaggg cacacctcgg gtacctggtg cttaccgact gattgcacca actcaattgt
     3721 tgccttgcat aagcggcatc tgtactcata tgacggcatg agcggactcc tctatatcaa
     3781 gccatccaag cccgcaagga tcttgaacag ctcatcttcc gttatctcta ttcctttgat
     3841 tgtcacaagc tgaccgtcaa ggaggaacat cttcattaat tcagcaatca cgtatttctg
     3901 attcgctttt ttcaagttcg cgatgtagcc gctgcgaacc atctgcgaaa gcgtcttcgc
     3961 cttcttctca tcgcccatcg tgatgaacgc attcaggaca tacccgcctt cttcacttcg
     4021 atcaaagagg agaagaatcg actcgctttg gcgaaccacc tcttcactga ctcccagatt
     4081 gagatcgaag aatgtccttg ggtactcggc gtagagggct atggcgcttt ctttcatcct
     4141 ttcggcgatc tcggcggaaa gccgcttccc gtcttctttc gaaagttgct catacacagc
     4201 cgggtagtca tcgagggtga acaggagctt gccgtttttt ggcgaataca gctcaagatc
     4261 gctttccttc atttggaagt aggaagggtc ttcatgaaga tcttttttcg tttttgaaga
     4321 ccacatgagt gcggtgttca tggcaactct tgagtacgtc gtctccatca ccccatagat
     4381 ccggcttgcc tcgaggttca acggatacgc atcatcctcg ggctcgatga cgacactgat
     4441 ccgttcgctt cgatccgcaa catcgggaag aaccgcttga atgattccgg gatcgttggt
     4501 cgtaatcaga atcgtccgat gcgtcggcag ctcttcaatc gtgaaggccg gccgcttgcg
     4561 caccgttgtc gtggtgcagc taccaagcaa catcaccgcg aacagagcca acagtacaat
     4621 ctttgctttc ttcatcatac cttcttgagc tccaaacgaa cttgatattc ctgcagttga
     4681 agggcttccc cgtcaagcgg ctttgaggta atttcatccg caagcgtttc atcgctgatg
     4741 atcgattcaa acttcgtaaa aacgtcgtgg atcttatcat caccctcata agcgatcatg
     4801 atccggtcgg tgacgtcaaa cccgctctct tttcgaagat tttgaatcgc tcgaataaga
     4861 tcgcgcatca acccttcatc cagcagctct tcggtcatcg cggtatcgaa gccgacggtg
     4921 agcgttccgt cgttgatcac cttcactcct tcgatctcgc ttcgctggac gataatttct
     4981 tcaacaccca gatcaatcgt accattggag taatgaagcg ggtagacccc tccgtcaaga
     5041 atgcgggcaa tcgtctttga atccagttcc tggatcaaag ccgccacttc cttcatgtct
     5101 ttaccgagct ttccgccaag cagcttgaag ttggccttcg cgctgtaggt gacaagcgca
     5161 gtttcatccg cttcgattga aacgctcttc acgttcagct cttcggcgat gctctcctgc
     5221 attgaagcga gcactttgcg ctcatgttca ttgcgatcga cgaggaagag cttggagagc
     5281 ggctgccgga tcttcagggt gtgagcggcc ctcagggcac ggccgagggc gatcgccttc
     5341 tgggtgagcg ccatctgctc ctccaaaatg aagtttcgct cctcttcccg gtacaccgga
     5401 taatcgcaaa ggtggatcga ctcgggcagc tcgttggaac ggaggttctg atagatctct
     5461 tcaacggtga acggaatcac cggagcggca accttgatga acgtcatcag ggcatggtag
     5521 agggtatcat acgcctgctt cttgtcctca tcgttctccc ctctccagaa ccggcgccga
     5581 ctgcggcgga tataccagtt gttcaactca tccaggaacg gaatgaaata ggaacatgcc
     5641 ttttggatat cataggcatc gaaggcggcg gtggcgtttt ccaccagccg ggcggtcgcg
     5701 ctgacgatcc actgatccat cggattcttc agatccgcat ggttcgttgc ggacggctcg
     5761 taggagtcga tattcgcata ggtgacgaag aatgagtacg cattccacag cggaagcaga
     5821 agacctttaa ggacatcctt gaccgcctct tcgctgaact tcaggtcttc cgcccgaacg
     5881 acggcgctgc tcatcaacgc gaagcggaga gcatccgctc catactgatt gatgagttcc
     5941 atcgggtcgg tgtagttccg ctcgcttttg ctcatcttct tgccttcggc ggtcaggacg
     6001 attccgttgg tgatgttgtt gtaaaatgcg ggtttttcat acaaaccggc cgccaatacg
     6061 gtaagggtgt aaaaccagcc gcgggtttgg tccaaccctt cacagatgaa gtcggcgggg
     6121 aagtgttttt caaaatactc ttggttttca aaaggatagt gcagctgtgc atacggcatg
     6181 gacccggatt caaaccaaca gtccaagaca tcggggatcc tccgcatcgt cgatttgccg
     6241 tcgggactcg gccaggtaag atcgtccacg aagtgcttgt gcagatcggt gactttctga
     6301 ccgcacttcg cctccagctc ttccaaactg ccgatcacct caatgtgatt tccatcatcg
     6361 ctcttccaga ccgggatcgg attgccccaa aagcggttgc ggctgatcga ccagtcgcgg
     6421 gcgccctcaa gccacttgcc gaagcggccg tacttaaggt gttccggcat ccagtagatc
     6481 tgctcgttcg ctgcaagcat gaacggcttg atctgatcga tatcgacgaa ccagctgctc
     6541 atcgcccgat agatcagcgg catcttggtt cggtaacaga acggatacgg gtgaacgatc
     6601 gtttcttcga ggaaaagcag attatgctcc tttaaatact cgatgatgtc gtcatcgcaa
     6661 tccttgacga accgccccgc atagtcggaa acctcttcgg tgaaattgca ctccatgtcg
     6721 atcgggcaga tcgtggggat tccggttccc ttcaacacct gatagtcatc ctcgccgaaa
     6781 cccggggcgg tatgaacgat gcccgatccg tcttcggtcg taacgtaatc gcccagaacg
     6841 gtgacaaacg ctccgctctt ctctttttca tcggcgaagt acggaaagag cggctcgtag
     6901 tgcagccctt catagaagga gccctttcgg gtatccacga cctcgtacag ctcttcatcg
     6961 gtgtagtagt gggacagccg gtcttttccg aggatgtaat agttgccgtc ccgcttgtcg
     7021 agaaccttca catactcgat ctgtggcccg aatgcgagag cgaggttgga aggcaatgtc
     7081 catggtgtcg tggtccaagc gaggaaatag gtgttctcct cgccgtcaac cttgaaacga
     7141 acggtgagcg cgggatcctt gacatcctgg tatccgccga ggttcacctc gaagttagaa
     7201 agcggactgg caagcttcgg actgtaggga agaatattgt atccttcgta gatatatcct
     7261 ttttcataga gcgtcttgaa cacccaccag atcgattcca tataggagag atccatcgtc
     7321 cgatagctgt cctcccaatc gacccagcga ccggtccgtt tgatcgtgat ctcccactcc
     7381 ttggtatagc gtaatacgat tgagcggcac atctcattga acctggctac gccaaactcg
     7441 acgattgccg aatagccgct gatcccttcg ctcttttcga tctcatattc aaccggaagg
     7501 ccgtggcaat cccacccgaa gcggcggttc acccgccttc ccttcatcgt ctgatatcgg
     7561 ggaatcgtgt ctttgatcgt cccgggcacg atgtggccaa agtgcggaag accggtggcg
     7621 aagggaggtc cgtcatagaa gacgtactct ttttcttccg ggcggtattc aatcgacttc
     7681 ttaaagatat tccgctcttc ccaaaactga agaacccctt cctccatctt gggaaaatct
     7741 actttcggat taacgggacg aaacatattt gctcctcgcc tcaatcgggc cttgtgtatc
     7801 agtgtcattc tattcattca tcgcataaat gacaaccatg aatagaccgt tgacctcatc
     7861 ccgatgaggg agtactgtca tcatatgcga acctttccga taaccggcag aataaaacgc
     7921 tttgcctccg tcttccgtga caacggctac tctctttttg ttgtcggcgg tgcggttcgt
     7981 gactttctct taggcttggg gaactccgat ctggacttta ccaccgatgc ccgtcccgaa
     8041 gaggtgatcg ccctctttcg aaccgtcatt ccgacgggaa tcgcccatgg gaccgttacc
     8101 gtcatctttg agggggagca ctacgaagtt acgacgttcc gaagcgaggg ggaatacaag
     8161 gatggccgaa gaccctcttc cgttaccttc attccatccc tggaagagga cctgaagcgc
     8221 cgtgacttca cgatcaatgc attcgccgct tcggcgatca ccggaaagat cgtcgataat
     8281 cacaacggtg aacaggacct gaaagacggg atcatccggg cgatcgggaa tccctacgaa
     8341 cgctttgatg aagatgccct gaggatcatg agggccgctc gaatcagcgg aaaactcaat
     8401 ttcacgattg aagaccaaac cttcaatgcg atgggtgcat tgaaagagaa cctcaggctc
     8461 gtttcggttg agcggctgca tgatgaactg gtgcacttgg tgaagagcga ccatccaaga
     8521 aaaggatttg agtaccttca tgcaagcggt gcccttgcgg tcattcttcc ggagcttgcc
     8581 caaggcgacg gagtcctgca aaaagggttc caccatgagg acgtgctgca gcactccttg
     8641 accacctgcc aaaaagcggc ggatctgggc gcctcggtat atgtccgcct tgcagcgctg
     8701 tttcatgaca tcggcaaagc aaccacccgg gaaccgaacg gcgaccgcta ctctttttac
     8761 aaccatgaga tcgtgggaga agcgctcacg aaaacgatcc tcaggcgcct gaaggcctcc
     8821 aacgaggaga ttgataccgt ctcccatctg gtgcgccacc acatgttctc ctacaccccc
     8881 gagatgacgg acagtgcggt gcgccgcttc atcgttcgag tcggaatcga gcatattgaa
     8941 gacctcttca agctgaggat cgccgatcaa atggcgatca gcggccgggc cgataccgat
     9001 cttctggatg aattttccaa gcgaatcaac ggaattatct catccgacga cgccctctcc
     9061 atcaaagatc ttgcaatcgg gggaaacgac ctcttggcgt tgggaatacc caagggcaag
     9121 cggatcggtc aaacgctcca atacctgctg gagaccgtac tggatgatcc cgctcaaaac
     9181 acgaaagagc agctcttcag gatcgctctg gcctatcagg agctctgtgg atttaccagc
     9241 gattgatatg cgattttagc caccgcctgc tcggcggcct tcttgcttcg cccatgggcg
     9301 ggaccgaaga cttgtccgtt cacgtccact tcgacataga aggtgaagtt atgatcgggt
     9361 ccggtttttg acacgagcct gtaggtgggc acgattcgcc acttcttctg catgtactct
     9421 tgaagagccg ttttatagtc gcgatgataa tcatcttcga cgacagcggt gatccgttct
     9481 ccgagggctc gactgacgaa cgccttcgcc gccggaaagc cgttgtgcat atagcacgcc
     9541 ccaaagagcg cttccgtgca atccgccaag agcgctttct ttctccgccc gccggtctgc
     9601 tcctccccct ttcccagaag gagatattga gcgacgttca gtttcttggc ggcgagagca
     9661 aggctgtctt cgctgacgac gatcgatttg atcttggaca gctctccctc ctctttatcg
     9721 ggatatgctt ccaagagcag atcggcgacc gcaagaccga gaatcgaatc gccgaggaat
     9781 tcaagtcgtt cattgttccg ggtccggtcg cccgtctcgt tggcatggga acgatgggtg
     9841 aatgcaagat cgaggagcgc aagatcatcg attccgatcc cgcttgcttc attaaatgca
     9901 agaagctccc gttctcgctc tggggcaaga gagggagctt ccttggtacg cagctcgtcc
     9961 atggaatttt aaagttcgga tgcgaggaac tcgacagcgt ccttgacggt ttcaaactcg
    10021 ttcgcttttt cgtcgggaat ggagatacca agctcttctt caattgcgta taccagttca
    10081 taggtatcaa ggctgtcggc accgaggtcc ttgcggaagg atgcttccat cgtgatcttg
    10141 gattcttcga tctcaagctt ctcagcgatt agggctttga ctttctcaaa tacttgcgta
    10201 ttatccatag ttaactcctg atgttcttat ttatcttgca gttcaatgac ctgcacaccc
    10261 cgataaaagc cgcactttgg gcatactcgg tgaggaacga ccatgttccc gcacgtacca
    10321 caacgtgaaa gtgtaggagc ggcaagcttc atattcgccg atttgcggct cgccgatctt
    10381 gcctttgaag tcttatattt gggtgttgcc ataacgtagt cctcttattc ttttcaaaat
    10441 tttgacacgg ttggtaggat agctaaaaac aattaacgcg tcaatcaccg ggtggttaca
    10501 ccttaaagac atctcattat ccaaaaccaa tatgacattt ttaaagaatg tgtaaatata
    10561 acatatcaag cccctttcgt tcaagaggct gctctcgata tatcacaatt caatataaga
    10621 gtttttctta ctctttagcg gccttgagcc gttgtttcat gatttgagcg accaccgggg
    10681 gaaccatcgt agagatatca gcgccgaacg ccgccatttc cttgattgcg cttgagcgaa
    10741 ggagaaagta tttcggactg gtgggaagga agaggacttc gaggcccgga tacagctgct
    10801 tgttcgtcat tgcaagttca aactcgtacc cgaagtcggc gagcgcccgg acgcctcgaa
    10861 ccatcactcc gaccttgtgg gtttttgcaa aatcgacgac gagaccgctg tgagccacca
    10921 cttcgatgtt ttcatgctct ctcaaaatct ctctgagcat tgcaaggcgc tcttcggggg
    10981 tgaacagata ggatttatgc acgttatctg cgataacgac atagagccga tcatagagcc
    11041 gggcgcttcg ttcgatgata tcgacatggc caagcgtcgg gggatcgaag gatccgggaa
    11101 gcatcgcggt acggatgttg ctgttcacgc tttcatctcc tcaacgtatt ttttcatcgc
    11161 cgcaaaggat tcgggggatt cccattcgat tctcttgacg aatgtgtacg acccttcgct
    11221 gttcttttca gcaaccgcga acattccccg accgatatag ctgatcgtct ggttctcctt
    11281 caactgttct ctctcggtga ggaaaccaag cacacagtcg aaatggcagt atccgccttg
    11341 cggatgggta agcgcgttgg cgatcagggt gatcggcttc ttgcacatca cgcagacctc
    11401 tttcggttca acaactttct tgacgggaag cggaggcaag gtcggagtga acttcttctc
    11461 acttggttga acgaaaatct cctgctcgac tttcttttgg ctccctttcg ctttccgatt
    11521 tcggcgattg ccgcgccgac gatccttttt gggcttcttt acctgactgt ccatagacca
    11581 agcgtactct cttttacccc gaacgacaag ctgagggaca gcaaacatgc tgtccctccc
    11641 tgtgaatatc tgtcaagact cgtcttaggc gttcttcttg cggataagct ccttaacctt
    11701 tgctttctta ccgacccgct gccggatata gtagagcttc gctctccgga ctcgaccgag
    11761 acggacgacc tcgacacgtt cgactcgagg cgagtgcaac gggaagatcc gctcgacccc
    11821 gaccccatag gaaatctttc gcacggtaaa ggttcggcga actccgctat tgttcttcgc
    11881 gatgaccaag ccttcaaaca cctggatccg ctcggttgcc ccttcgacga ttttaaaatg
    11941 tacttttacg gtatctccga cggagaagtt ctctgcattc tccttaagtt gtactgattc
    12001 aacagctttc atcacgtcca ttgtctacga tccccttcgc catatgttct ttcaattcat
    12061 ttacttgttt tctggaaacc gcatccaaag aagctgtttc caacagatca atccgccgct
    12121 ccatcgtccg cagcagcttc tgtttcagtc gccatgattc gatcttctca tgatgaccgc
    12181 tcaataatac atgagggacc tcttttgggc aagagggttc gcttcgcgtg tactggggat
    12241 attccaacaa cccgtcactg aagctttcct catcaagcga ttttctactg atcacgccgt
    12301 cgatcagccg gaagaccgaa tccacgatca ccatcgtggc gacttcgcca ctggtgatca
    12361 cataatcgcc aatcgagatc tcatcatcca catactcgtc gatgattcgt tgatcgaccc
    12421 cttcatagtg cccgcagatg aagataagct cccgctcctc actgagttca tgggccagtt
    12481 tctggttgag cagcaccccc gacggtgagg gatacaccac tcgcttgccc cttgtcccca
    12541 gctcatccaa tgctttggaa agcggatcgt acatcaacac catccccgcc ccgcctccgt
    12601 agggaaggtc gtcgacgctc ttgtgcttgt cagttgccca agaacgaaag tcgatgagct
    12661 tgtacgctat tgttcccttt tcaaccgccc gcctcatgat cgatgaggtg aaaaacccct
    12721 cgagcatgtg gggaaagagg gttagaatcg taatcgtcag tcgaggagcc atggggataa
    12781 caactcaatc gttttcgctt cgagatcgac ttctccggta tactcgtaga gaaacggaac
    12841 caggaagcgc ttggaatccg gccgctcggc ctccagaagc agtgcctggg gcccatccag
    12901 cacactgaca accatggcaa gagcttctcc cccggacaca agggtgcacc cgatcagatc
    12961 cgcagtgtag tactcgcctt ccttgagcgg ggaagcaaac gcacggtcaa ccatcacccg
    13021 ggcaccgaca agggtctgtg cctcttccga agaatcatac cctttgaatt tcatcaaaac
    13081 gcttttgccg tccgctcgca catattcgac ggcaagtgtt gcctctttgc cgtctttcag
    13141 ggaagcgacg atcgtcttga gctttgtaag gtaatcggac tcctcattgt tgggatagac
    13201 tctgacctca ccctcgtaac cgaaggttcg cccgatcgta gcggtagcga tgagtcgatc
    13261 cacgtcagtc gaggatctcc agcactgccc gctttccatc ccgggtggcg gaggcgctga
    13321 gaatcgttct gatcgctttg gcgatccgcc cctgcttgcc gatgaccttg ccgacatctt
    13381 cgcttgcgac gcggagttcc agaatcgtgg acttttcgcc ctcgacaaca ttgacatcca
    13441 cctgatcggg aagatcgaca agcgacttta cgatatattc aaccaaatct ttttccacag
    13501 tgtgctcctg ggacttagtc ccgggctttg cgattcacct gcacgccgct cttgttgagc
    13561 aaattgcgaa ccgtatcgct tggctgggcg cccttcatca accattcttt gacgcgatct
    13621 tccttgatca ccaactgctc ttctttcttc tctgcaaggg gatggtagtg tccaacttcc
    13681 tcgattgcgc ggccttggcg cttctcgcgg gagtcgatca ccacaattcg ataatccggt
    13741 cgtttctttg atccaaacct cttcagtctc atgctcacca tcgtgacaat tcctccatga
    13801 tatccaacta cagacccatc tgttttaaca tctcagcctg gtatttttta tttcgcgtca
    13861 acttcctcat catcaggcgc gacttctcaa acttcttgat caagcggttc acatcgctga
    13921 tgtgcgtacc gcttcccttg gcgatccttc ggcgccgggt tggtccaagt ataaggtaat
    13981 tttcccgttc agcataggtc atcgaacgga tgattgcttt ctcccgttca atttcctgct
    14041 tgtcgatgtc atcttcactc acttgacctt tcatccccgg aatcaattcc aacatcgatt
    14101 cgagaccgcc catcttgttc atcgattcca gctgctcaag ataatcctga agatcgaagg
    14161 tgttcttcgc catcttctgc tgcatcttga tcgcctgctc ttcatcgaac acctcctggg
    14221 ctttctcgac aagcgagacg atgtccccca ttccgagaat tcgggaggcg taccgatcgg
    14281 catagaacgg ctcaaggtct tcgaccttct cgcctactcc gatgaattta atcggctttc
    14341 cgacaaccga acgcaatgaa agagcggcac ctccgcgggt atcgctgtca aacttcgtaa
    14401 ggatcactcc gctgatgccg acctgctctt caaactcctt ggcgatcgta accgcctgct
    14461 gaccggtcat cgcatcggcg acgaacagcg tttcctcggg cttgagcgca tcccggacct
    14521 ttttgatctc cgccatcagc acatcatcga ggtgcatccg gccgctggta tcgacgatca
    14581 aggtattgaa ctgctctttc ttcgcttttg caaacgctgc tttggcgacc ttcgccgggt
    14641 ccttttcccc ttcgatggaa aagacctcga caccgaccgt ctttccaagg acttgaagct
    14701 gcagaatagc ggcgggccgg accagatcgg cggcgaccag aaggactctt cggccctctt
    14761 tcttcaatcg aagggcaagt ttcgcagcgg tggtggtttt accggaaccc tgaagaccca
    14821 tcatgagaat cgacgagggg gtatcggggc ctttgaggtt gagcttctgg ttctcctcgt
    14881 ctccgaggag gttcaccatc ttgtcgtaga caatctttac aaactggtcc cccggatcga
    14941 ccgccttgag gaccttttca ccaagcgctt cctccatcgt ggaattgatg aacctgcgga
    15001 cgacgcggat attgacgtcc gcatccaaca gcgccacctt gatctcttca acggcgtctt
    15061 ggatgttctt ttcactgatc ttggattttc cagcgatcga tcgcatgatc gagctgaacg
    15121 cattgcttat cgaatcaaac attgtctctt aactctttca ctcaaattga ccttataccc
    15181 tacaccctca aaccctgtca agaagagcta acggatgagg cttccctctt cgttgaaatc
    15241 aacggcttgt tcatcatcat tgaggatgac cggttttccc tccagcgtat cgagacggta
    15301 gctgaccatc gtggataccc ccggctccat ctgggtcacc cccaaaagcg tcgtcgaacc
    15361 catgacggtg tgcttgttgt gggttacgat gatgaactgg ctcttctgcg caaactcctc
    15421 aagcagcgag aggaagtagc cgatgtttcg atcatcaagc gccgcatcga tctcatcaag
    15481 caccgtgaaa ggagacggtt ttacgctgta ggtcgcaaag agcagcgcca ctgcggtcat
    15541 cgagcgctcc ccgccgctga gcagcgtgag gtgggtaagc ttcttccccg gcggctgggc
    15601 gagaatatcg atgccgcttt caagaacatt atccggatcg accaacgaga gctcggcgcg
    15661 gcctcccccg aaaagccggc ggaacatcgc ttggaaagcg gctgaaatct ctttgtatgt
    15721 agccatgaag agctcttcgc tccgcgtggt gatctcggtg atgacgctgt ccaaatccgc
    15781 ttttgctttc gccaaatcat ccaattgcct tgtgaggaag tcatactgtt ccttgatctc
    15841 actgaactcc tgctccgcca tctggttgat gtaccccatt ccactgacgc gcttcttcac
    15901 ttcttccaaa cgcgctcgaa gtaccgggct gtcatccagt tcttcctctt caagccgatg
    15961 ttcgaactcc ttcaagcttt ttccgtatgt atcgaagaag ttggtataga gcccggcaat
    16021 ctgctcttcc agattctggg cgtgcagctc cgccttttcc cgctcgcttc gaaggttctg
    16081 gatctcatta aaggcatcct ccttctccgt ttcgcggttt tggatcaata ggttttgctc
    16141 ttcaattcgg gtggcaagct cttcaagctc ctgattcaaa tcgacgatgc gcgtcttgat
    16201 ctctttcact tccgcttcgg tggctcgaat atcctcctgg gtttcatgga tccgctcccg
    16261 ggcgttcgtt gcagctgcca gagcgtcttt gtattcatac tcccgttcgg taatcgtgct
    16321 tcgaaggttt gcgatccacg cttgggcgga ttccttgttg gaacgaagct ggctgagagc
    16381 gctttgatgt tcggtgatcg catcttgaaa gagatccatc tgactctgga gatgaaggtt
    16441 ctcctccctg aggtgggaaa cccgctcccg gttacggatg atctcttttc gtttctgagc
    16501 gatcgccaga tccaatgatt gtttttttgc gatcgtccct tcgggggcga tgagctcatc
    16561 caggaaagat gcctgagttc cttcataggc ggtaaaatga ccggaaagtg aagccaatga
    16621 gtcgcctaag gtggaaaaaa attcatgttg ccgtttgaac agtgtctcga cggaaaggtc
    16681 ggcggctttc agcttggcga gaaactcctg ttctttgaga atcgattgct tgatatgctc
    16741 caactccgac aagagaatgc ttcgggcttc atttcgggct ttggtggtat atccgcttga
    16801 acgcagttgt tcttccagct gaatgacgat cgcatcactg agttccttga cctcaaggct
    16861 cagcgtgtcg gtctcttctt cgagccgggc attgctcttc tcgagtttgt tgatctgatt
    16921 gatatgatct tcgatcgtct tccggttttt cccaagggag gccagaaacc cttccaactc
    16981 cccctccaat tgcctgatct gttcatcgat ctgagtgata tgttcggtcc gctcggcaat
    17041 atgcgctttg tcgcgattga tctggccttg aatcccctca ctgcggcttt ttgcgctttc
    17101 ctgctgattc aggaaatccc ggtaccgcac cgtcaacaga tcaagttttt cgccccttcc
    17161 cttcgtcgct tcatcaaggc gctggagctc ggtctgaata gcgatgcggg aggcgcccaa
    17221 ggtcctcagc tcttcgcgca gattggcgag gttctcgtcg aaggtttcca gttccgcccg
    17281 tttcgcctga tagtgccggg aaaacgtccg ctcctgttcg agccggacct ccctcagctt
    17341 cgaatagctg accagggtgg aaagctggat atcgacttcc agagtgaact gctcttttcg
    17401 aagctcccga tagttcttcg ctttttccgc ctgtcctttc ttcgtatcat agtttcgctt
    17461 cacttccctg agaatcgttt ccacttgagc gatgttctca tcggttcgct ccagcttgcg
    17521 cttcgcttcc agactttgca ccttgtatcg gctgattccc gccgcttcct cgaagatgta
    17581 gcgccggtcc tccggccggg aggaaaggat ctggtcgatt tttccctgct ccagaatcga
    17641 gtaggcgctt ttcccgaccc cggtatcgaa gaagagatcg cggatgtcca tcaaccgggc
    17701 gcgattgcga ttgatatagt actcgctttc cccggtgcgg aagatccgcc gcttgatctc
    17761 gacctccggc tcgtcgatcg gcagatcgcc gttctcgttg gagaggacga gaatcacttc
    17821 ggcgacctga aggggtttgc gcaggtcggt tccattgaag atcacgtcct ccatcttcga
    17881 tgcccggagc gtcttggtgg attgctcgcc gagaacccat ttgatcgcat cgacgatatt
    17941 ggatttgcca cacccgttcg gtcccaggag actggtgatc ccctcggaga attggagctt
    18001 cacccgatcg gcaaatgatt tgaatccata gagttcaagt gatttaagaa acaacgcaca
    18061 ccctcttctt atcttgacga tatctgagag cgattatagc atatcaggcg gcggaggtaa
    18121 tagggtatga cgttgatctg tggggttgat gaagctggtc gaggccctct ggccggtccg
    18181 gtggtcgccg cagcggtaat cctgggaaag cacttccctg cccacctcct gaaagattca
    18241 aagaagctgg cccctaagca acggacgatg cttgaaacgc tcatcaagga acaggctgca
    18301 ggttggacgg tggccgtcgt ctcgcatcgg cggattgatg agatcaatat tcttcaggcg
    18361 acgatggaag cgatgcacaa cgcagtatct caattacaga aaaaccaccc gatcaccaaa
    18421 gccctgattg acggcaatcg ctcgccgatc ctctcctgcg aaacggagac aatcgtaaag
    18481 ggggatgact cgatccccga gatcatggcg gcttctattc ttgccaaggt ggaacgggac
    18541 cggatcatgg tcgcctacga tgaacagtgg ccgatgtatc gcttcgccga gcacaaagga
    18601 tacccgacca aagcacataa agaggccttg aaacaatacg ggccttctcc gatccatcgg
    18661 ttgagtttca gatattaacg ggtattctgc ttgatgaaat cgagggactt ctgaagctct
    18721 ttcaaaaact cgcccaagtc ctcctggtag atgatgatcg attggcgaat aaatcggtca
    18781 ctgtccggca ggcctttcga ttcgacgatg ttcaaaaaca catcccctcg aatattctct
    18841 ttcacattaa aaaaatatgt ccgctcattc ttgacgagcc ttgttgaaaa cacttccccg
    18901 cgttgaccca cgctcctacc ctcgcttcag atcgatagat ccatctcttt tgtatcagtg
    18961 atgtcttata aatgtcaatc gacatcaaac gcggaattct cggggtactt gacgaagatg
    19021 aaacgataca ctataaaaag aggcggttga tgcgcctttc gtataatggt attacctcag
    19081 ccttccaagc tgaagaagcg ggttcgactc ccgtagggcg ctctagagag gcactccgat
    19141 ggggtgcctt tcgtgtcctc gtaaggagtg ctcatgcagg aacgctacgc tgaactggtc
    19201 atcgccatcc aactccgcct aagcgaggga gacagcctta cgatcaacac caacgcccgg
    19261 acgatgagct tcgcccggct tgtcgcaaga aaagcgactg aagtgaccaa actgcctgta
    19321 acgattgtcg agacatcaat gggcaaagtg cttcagacct atccgatcga ccctgttgaa
    19381 aaaggggtca tgcgaccaca ggtacgtacc tccgttcttg cccatatcgt cgatctggat
    19441 gaattcccct acgatgaaga cgaggattgg aacgagccgc atcatgaagt gcgaaccctc
    19501 gcccactacg gccatctttc cgatccggtc ttcctggatc gaagaatatc gattccctgg
    19561 gcgaacatcc cctacccggg aaccgcctgg gcggcaagca tgctcggccg cccggtgagc
    19621 cgcgaagaga tgtggagcct gtttggaatc ctgatgcgcc ttacctccga ttggtcgccc
    19681 gcctactggg cggagcaggc aaacgtgctc tcttatcggg aaaagcttct caacgcctct
    19741 tcaggaagga tgatctcgat caaaggaagc aacacggagc tcatcggcga acaaagcccc
    19801 ctgaccggat ggatcagcgg ggtgttccgc ctcccctcgg ggcgcgagta tattccgctt
    19861 cttccggtcc agtcggttca tacggcgttc aaaaccggtt cggtgaatgg aaagatccgc
    19921 tcgggatcgc cgtttaccct gttcggcaca ccggtgacgg atgccgcctt catcattgaa
    19981 aacggagttg tggtgtccta tgacgcccgg gaaggcaaag aagcgctcaa gaagtatttt
    20041 gacgtggatg cgggaaccaa cgcggcaagc ggaatcagtc ttgccgattc caacaccctg
    20101 gagtgccgct atatcacccg cctcgtccat ccccattaca aaaaagagtg ccgctcgatc
    20161 cttcatctcg gaggcgtgct ggtggatgcg atcaaagcgg gaaccgggga ggatgacctg
    20221 tttggccttg gacaatcaat cgcccacttg gaaatccccc tcgcccatga tattacagta
    20281 gagatgcaaa agcccgacgg ctcaatgaag acaatcattg ccgaaggatc tttcaccgta
    20341 taggaggaga tacaacgatg actgaacagg atatccgacg atatgcggaa ttgatcatca
    20401 ccaaaggcat caacctgcag aaaggcaagg cggtgctcat caccaccgga gcggggacct
    20461 actatttcgc ccgcgccctc ggcaaggccg cgtaccgcct gggggcttcc tatgtgcaga
    20521 ttctcaccga tgacctcgat ctgctctctt cccgcttgga agcccaaagc gaagaagagg
    20581 tccaattcgt tcccgcctac ctcaaggcgc tcgattatga attcatcagc gaaggatggt
    20641 cctccatccg catcgattcc accgaggagc ggttggacca cggtccgctc gatcctgaga
    20701 agaaccggct cgcttccgcc gcccgccgaa aggcttccga agcccgctcg agggttctga
    20761 tgcgccatca gctgccttgg tgcgtctgcg tcgccccggg accgctctgg gcgaagcagg
    20821 ttcttggcga ggatgccaag cctgaggatc tttttgaggt gcttaaaccg attcttttgc
    20881 tggacaggga agatcccgga acggcttggg atgaaaaaca cgagacgctg cagaaacgga
    20941 tggactttat caacgcgttg gatttcaagg cgctccactt ccaaagcccc cgaaccgacc
    21001 tgacgatcgg gttcagaaag gaagcccgat tcgtcggcgg agcggagacg cttccctccg
    21061 gcaagcgctt cttcgctaac cttcccaccg aggagatctt ttccactccc gatcggatgc
    21121 aggtcgaagg gtatgtgacg acgacccgcc cggtggaggt gctgaacact cccaccgagg
    21181 aggttcgtct tgtcttcaag gaaggaaagg ttgtcgagca ttcggcgaag aagggtcagg
    21241 aagtgttgga ccgtttcttc gagatcgacg agggtgcgag gaggatcggc gaagtggcgc
    21301 tggtcgatga aagcagcccg atcgcccaaa gcggcttgat cttccactcg atcctgcttg
    21361 atgagaacgc ttcctgccac cttgccctcg gggaaggata tccgatctgt ctggaacacg
    21421 gttccacgct ttcaaacgag gaggagcttc acgaagcggg gtgcaacacc agcctggtcc
    21481 acgtcgactt catggtgggc tcaagtgaca tgaacgtcga tgcgatcctg tccgacgggt
    21541 cgagagtccc gatcatcgtc gatggccgct tcagtttaaa tcactgatca gtttcaaccc
    21601 gtcaagggtg tgcatcggcg caatctgatt gattcgtcca accagggggc tgagcgttcg
    21661 cgaaagaccc ccggtggcga tcacatagag ctcttcgccg atctccgctt ccgtcagctc
    21721 gatgatcgat ttcaccaacc ccgcatatcc gaacatgatg ccggcgttga tcgaatcctg
    21781 gctggtccgc ccgatcaccg aaggcggggt tttcaattcg acctgaggca gttgagcggt
    21841 cgctccgaag agggagttca ccgctgttac caatccggga gcgatcgaga ccccgagaac
    21901 cgctccctcc ccgttcaccg ttgaaaacgt cagtgcagtt ccaaaatcga tgaccatcac
    21961 acatctatcg gggtgaacgt agtgggcctg cgccacgttg gccaagaggt ccatccccag
    22021 ctccttgggg atcgtctcgc ggatcagatt ggtgttcacg gaatggctca ccatcagagg
    22081 ctggatcgag aagaggttgt agatgttttt ggcgatcgcc cgggtgaggt tgggaacaac
    22141 gctgctgatg accgctttgg tgatatcctg acggctgatc ttggcattgt tcatcagcgc
    22201 ctcgaggacg acgaagtatt cgtcggtcgt cttcttctga tcgctatgaa tccggaaaga
    22261 gtggatccac tcttttccac tgtgcaccgc aacaacgata tggctgttgc cgatatcaac
    22321 cgctatcaac atggcacctt cctcagagcg ccaaagcgtt cttcactcct ccgtagagtt
    22381 cggttcggag cgccttgatt ccctcgtcct taaaatactc ttcaaagctc atcattctgt
    22441 caatcactcc gcccggagtg aattcgatga tccgattcgc gatcgattcg acgaactgat
    22501 ggtcatggct gttgaacaga atatttccgg tgaattcgat caatccgttg ttgagggccg
    22561 tgatcgcctc aaggtccagg tggctggtcg gttcatcgaa gatcaggacg ttcgccccct
    22621 caagcatcat cttcgcaagg agacagcgga ccttctctcc cccgcttaac acggtaagat
    22681 ccttcagcgc ttcatcaccg ctgaagagca tccgcccgag gaaggaacgg acgtaggtct
    22741 catcctgatc gggactgtac cggcggagcc aatccacgat cggaacatgc tcctcaaaga
    22801 gatggttgtt gttcttcgga aagtacgaaa gactcgtggt gacgccccag gtgtaggtac
    22861 cgccgtcctg tttgtcattg ccgctgagga tctcgaagag ggcgctcttg gcggcgtggt
    22921 tgggcccgac gaacgcgatc ttgtcgccgg cgttcacgtg aagagagaat ttgtcgagca
    22981 gtacctctcc atcgatcgat ttggaaaggg agtcgacatc cagaatattc cggcctactt
    23041 cccgattggg cttgaaggcg acatagggaa agcggcggct ggagggacgg atatcatcaa
    23101 tcgtgagctt gtcgatcagt ttctttcggc tggtcgcttg acgggctttg gcgacattgg
    23161 aactgaaccg gtagatgaac tccttcagtt cggcgatctt ttcctcccgt cgccgtttct
    23221 cgtctcgctg ctgttttgcg gcgagctggc tggaaagata ccagaagtca tagttgccga
    23281 cgtacagctg gatctttccg taatcaatgt cggcgatatg ggtgcacacc gtattgagga
    23341 agtgccggtc gtgggagacc acgatgacgg tattgtcaaa gtcggaaagg aagtcttcaa
    23401 gccagtggat cgactccaga tccaagtggt tcgtcggctc gtcaaggagg agaatatcgg
    23461 gattcccgaa cagcgcttgg gccaacagag cccggacttt gatcgagtct tccacttcag
    23521 ccatctgttt ttcatggagc gcatcggcga ttccgagtcc gctgagcagt tgagccgctt
    23581 cgctctccgc ttcccaccct ccgagttcgg cgaaatcgcc ttccagctcg gcggcgcgca
    23641 gtccgtcctc ttcggtaaac tcctctttgg catagatctc atcccgttct ttcatgatcg
    23701 agtagagctt ctcatatccc atgatgaccg tatccagaac cgagtactcg ttgaatgcga
    23761 agtggtcttg gcgcagggtt gcaagccgct ggccgggggt aatgatgacc tgaccggtat
    23821 ccggttcgat ttcaccgctg agaatcttca aaaacgtgga ttttcccgca ccgttcgctc
    23881 caatgatccc ataacagttt ccgggggtaa acttgatgtc tacctccttg aagagaactt
    23941 gcgtgccgta tgacaggcct atgttggatg ctgtaatcat cgatgtttcc tctgtcggaa
    24001 aaacagggca accggtggtt gccctgcttc acactcgtag ttcctagggg aatcgaaccc
    24061 ctatttgaag actgagaatc tcccgtccta accgttagac gaaggaaccg atcgctggga
    24121 tggagggatt cgaaccccca aaggcagaac cagaatctgc agtgttacca ttacactaca
    24181 tcccaatttc caaaatcctt cggtaggata cgaaaatcct caatcctttg tcaaccaccg
    24241 caagagtatc ggtcggcgga acaactgcac gctcactgta ccctccaaat gaggtgtttg
    24301 tcaaaggtca ctcggagtcg tatgtgataa gaacttccgt cggatacgct ttaagcactt
    24361 cggtgtagtt caggaagggc aatccaatga cccccaggat gccggtgacg atcgctccgt
    24421 tctcgttgaa gatcgaggct gcggcttcaa gggttccacc ggtggcgatc aagtcgtcga
    24481 ccaacagaac cttcttgccg ggatgcacat cgagtttttg gatgcagatg acatcttccc
    24541 cgtattcgag agcgaagctc ttgctataga cctcgttggg aagcttgccc ttcttgcgga
    24601 tgaggatcaa cggaacaccg aggcgctcgg ccaggggagc cccaaagacg aagccgcggg
    24661 cttcaaccgc tgcaatataa tcaaattggt aggaacccgc tctctttgca agctcatcaa
    24721 tgctggcacg aaatgcgttg ctgtcattca aaattcccgt aatgtcataa tacaggattc
    24781 cttctttcgg aaaatgggga acttttctaa ttgcaaggtc cagatcgatg ttcacactca
    24841 gcctcccggg gtgtactgtt tcttaaacgc actgacacgt gcacttgctt tcatatcata
    24901 gcggtcccgg attccatcct tgaggtagtg gtatcccaga gcggcgatca tcgccgcatt
    24961 gtcggtacat aactccatgg agggaaaagc gaccgtatat ccgccgctcc caaacttctt
    25021 cagttcgctg cgaaggtagc tgtttgcggc cactccccca ccgacgctga tgctcttcag
    25081 cccggtatcc gccaaggcgg ctcgaatccg cttgatcagc atattgattg cggttcgttg
    25141 aaaggaggcg gcgatgttcg cactcgattt ctcgctcttg ccatcccaat atttgtccag
    25201 atggtttatc acggcgctct tcaatccgga gtatgagata tcgtaggggt tcgtcttttc
    25261 gcggatcttc ggcccgggga agcggaaggc aaggggattg cctttttgag cgagccggtc
    25321 gatcgctatc cctcccggat acccgaagtc atagtgtttg gcgaccttgt caaatgcctc
    25381 gccgatcgca tcatcgatcg tggtccccaa cacttccaag tcatcatagc cggtcacctt
    25441 gcagatcacc gtatgtccgc cggaaacaag aacccccaga tagggatagt caagctcgtg
    25501 ttcgatctga ggggcataga ggtgtgcccg gatatgatcg attccgatca ggggaacccc
    25561 tttggctgcg gcgtaccctt tggcgaagct cacacccacc aggagcgagc cgagaagacc
    25621 ggggcggttg gtaaccgcta caccatcgat ttcgactccg gcttcatcga tggcggcttg
    25681 aacggtcgga gcgatccatt cggtatgaat tcgagaggcc agctcgggca cgactccata
    25741 gtagatccgg tgctcctcgg tctgcgtcgc aatgacattg ctgagaatcg tttttccatc
    25801 gacaacgacg ctcgctgcgc actcatcaca ggaggtctcg attccaagga cgttcatgcc
    25861 tcttcatcct cggcgaccat cagcgcgatc tcttccagag cgaagccctg ttcgatcgct
    25921 tccggataca tctcaatgag aaaatcagcc acattcggat cccagctttg accgacgatc
    25981 ggtctgaaac gggctggatg gctgacgagt atttcaatta cttcacgggg tgctgcgggg
    26041 gtaaatacca tacgtttttt caatcaattt cctcagagcc agcctacttc aaacaccaat
    26101 actggtcaag ttatcccatc ccttgcataa aatcactcgg tacggtatta tgccctcgtt
    26161 atgccaaaat agctcagggg tagagcggct cactcgtaat gagcaggtca agggttcgat
    26221 tccctttttt ggcttcccat catcgggaac acgatgcatg aagactcgac accgccctcc
    26281 tgaagggctc catgtcgagt ctttccatat gcttcggcac cgttgaaggc tttgcggaga
    26341 tggttcgtgt attcttcacc gggcgtatgc tctgcgattg catgcacggc ttgcccattt
    26401 aagaatgtca gtaatctatc attgcttgat acatctctcc gatggaatat ttgcccttgc
    26461 tgttctcgct cattgccttt gctaccttca agctctccac gccgagatcg gagagaggga
    26521 gagatttcag ataggtcgtc agctccttca gtcgctcttt ggtcatctcc ttgtcgagcc
    26581 cgacttcccg tatcatttcc tgtatttttt tgtccttgtc catcgatggt gccatccttc
    26641 cgggagaaaa gaatcccggg ccgaagcccg ggaagattgc tacttctgct tgatcaccgc
    26701 t
//
LOCUS       DAOSLI010000024        25945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2104_length_25945_cov_26.604365, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000024 DAOSLI010000000
VERSION     DAOSLI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 25945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25945
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_25945_cov_26.604365"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1935
                     /locus_tag="PLF67_05010"
     CDS             142..1935
                     /locus_tag="PLF67_05010"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOE89406.1"
                     /translation="MKKQLTANELRQKFIDFFVSKDHKQISGASLIPENDPTVLFTTA
                     GMHPLVPYILGSEHPSGRRLVNYQKCIRTGDIEAVGDPHHLTFFEMLGNWSLGDYFKE
                     EAIAYSHEFLTTVLGLETDELSVTVFAGDDEVPRDEEAAAAWRKHGVPDERIYFLPRE
                     DNWWGPAGETGPCGPDSEMFIDTGRDACGPDCRPGCSCGKYFEIWNDVFMQYVKNADG
                     TYTEMERKCIDTGMGIERTIAILQGKRSVYETEVFTPIIKRIETLANKTYGENEEDDM
                     SIRILSDHVRTGVFILGDQKGVSPSNVGQGYILRRLIRRAVRHAHKLGIEGSFLSELA
                     EVVIELYKKPYPELLENREFVMKELVAEEAKFSATLAKGEREFEKMLPNLLKSPNRII
                     SGRTAFKLYDTYGYPIELTRELAAENGFEVDEKGFHEAFAKHQELSRAGGDQTFKGGL
                     ADHSERTTALHTATHLLHQALRTVLGDHVGQKGSNITVERLRFDFTHPEPMTQEQLQK
                     VEAIVNEVIRRDLPVSFTTMSLEEARQSGAIALFEGRYDEQVKVYSVGDFSKEVCGGP
                     HVERTGGMGTFKIQKEQSSSAGVRRIRATLE"
     gene            complement(1995..3035)
                     /gene="rlmN"
                     /locus_tag="PLF67_05015"
     CDS             complement(1995..3035)
                     /gene="rlmN"
                     /locus_tag="PLF67_05015"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOE89407.1"
                     /translation="MNLPLSLYGLTSERLVESLSLPEKRYGGQILAWLGKGATTFDEM
                     TDLPLDERKRLSDLVGSPISSETTVREEDESGTIKLGITLHDGKMIEAVLLVDRKGRH
                     TACLSVQVGCAMGCTFCKTGTMGLVRNLASEEIIEQYVHLSKAASRPITHIVFMGMGE
                     PFHNFDPTIRAIHYLNQKETFNIGLRRMTISTSGVVPGINKLAELKLPIKLAVSLVSA
                     DDSLRTRLMPVNKRWPLAELKEALLAYQESVPKRLTLEYCMLSGVNTTGESARQVELF
                     TRGLDVIVNLIAWNPIDELEFSTPSEEEQKAFSALLNRYRVKHTLRISRGRTIGGACG
                     QLATSHNPETKR"
     gene            complement(3049..4629)
                     /locus_tag="PLF67_05020"
     CDS             complement(3049..4629)
                     /locus_tag="PLF67_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOE89408.1"
                     /translation="MERHLTARAIIIGILGLLVITASSMYVALRMGALPWPTIFVTVL
                     SMAALKKARNSSLQEMNVTHTIMSSGAMVAGGLAFTIPGLWMLNPDAVFPPMSLITLT
                     VVGAILGTLFSALLRKRLIVEEELPYPMGIAAFNTLKASEEGGSGAKTLFTSMGGSVL
                     FTLLRDVWAKIPALLTPFKGSALFPSFSFWVSPMAAGIGAIIGPVFALFWFGGAIFGY
                     YLLTPIGIKAGLFGDISAADVFRQNLGIGLMIGTGLGVFIHALFSKILRPKKLEEKPK
                     KSQRTLFLISLVLLLAVVLLSLGTELGFVEALVLIVGIYLTTYLSGMLTGQTGINPME
                     IFGILVLLVIQLISKPSLLASFSIAAVVAVACGLTGDVMNDLKSGHLLGTNPRDQILG
                     EGIGGVIGAVVSVIVLLAMKHAFGGFGTALLPAPQAAAVAKMVGGIEHLPAFLIGLFV
                     GLALFLMKIPSATLGLGVYLPIYISSIMGLGALASLIFTRKKDKEKSQKKIGLIASGL
                     LGGEGITGVLIAILSMLK"
     gene            complement(4686..5963)
                     /gene="hflX"
                     /locus_tag="PLF67_05025"
     CDS             complement(4686..5963)
                     /gene="hflX"
                     /locus_tag="PLF67_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOE89409.1"
                     /translation="MSKPLHSLEHEMLSALLLILIDENETRQNADLRSEELASLVDTM
                     GLEVLHTEFIPLRGETSATLIGSGKVAEIKELLEELEADVVIFDRPISPRVQRNLEEI
                     FQSAVIDRDEVILQIFADRAQTKEAMLQVELARLQYSLPRLVRKWTNLSQQRGGVRGS
                     RGEGETRLELDRRRIQDRIALLKKQLLNVVQQREVQRSSRLKSPNPIGAIVGYTNSGK
                     STLLNTLTEAGVLAEDKLFATLDPTTRLFKLPGGEEVLLSDTVGFISDLPHHLVDAFK
                     STLEEARYADFLIIVCDASHPDMIATYTTTVQVLEDLGVTDKPAIVVANKIDKVEDFF
                     ALSRLKSLYQPVLETSLKSGQGLETLIDAITETIHALNPTLSYKFPNDRHDLVAYLHR
                     SGQVADISYTEEGIIVKARLAERFASSLNSYRV"
     gene            6041..7147
                     /gene="ychF"
                     /locus_tag="PLF67_05030"
     CDS             6041..7147
                     /gene="ychF"
                     /locus_tag="PLF67_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOE89410.1"
                     /translation="MGLNCGIVGLPNVGKSTLFSALTSAPAHVANYPFCTIDPNVGIV
                     AVPDSRLDRIAKLIPGKKKIPATVEFIDIAGLVAGASRGEGLGNRFLASIREVGVIAH
                     VVRCFEDGDVVHVNGNVDPERDIETINIELALADLDTVTKRYEKVSKGRSLSKEAQKH
                     ASEILPILERLLATLGEGGSARSLKLDEDQLSLISDLHLLTVKPVIYVCNVDEEGIDG
                     SSAHVDAVKRIAGEEDAEVVVICSKIESEIATLETEEEKQEFLEAVGLKESGLNKLIR
                     SAYYILGLRTFFTTGDDENRAWTFKAGMKAPQTAGLIHSDFQKGFIKAEIYSCDDLFA
                     YGSEQKLRVAGKLRLEGKEYLVRDGDVIHFKFNV"
     gene            complement(7156..7857)
                     /locus_tag="PLF67_05035"
     CDS             complement(7156..7857)
                     /locus_tag="PLF67_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89411.1"
                     /translation="MKTIHWIGILILVFLLAGCTVSQRLDFSPAPPKADFTFNVEEFF
                     VDVLEDFSDFSTKKSDRPVMDQAIDDFETLLDSAPSTKAVQLDKTGVNAWNGTFIFSD
                     VENLVRDLGAPKDQQLLKATNDSITFYLTLETYPQLVPIIPFLADENFEAFGPVYNQG
                     LSADDYLEMISFMLGEEGPPAIEQSTITLRVTTPKPIKSMINGRKLDPTTYEFSFPLI
                     DFLLLAEPITFTVTW"
     gene            7954..8739
                     /locus_tag="PLF67_05040"
     CDS             7954..8739
                     /locus_tag="PLF67_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOE89412.1"
                     /translation="MMRRMLFFLMIILIGSALHALDLHVDEIQPSRITLSWDRVPSAL
                     TYDLYLNGCYRATVRTPSYSIEHLTSHTSYSVRVQAKGQGTVIDEGTIEVTTSGWEGR
                     YQWVNRTRKDNRGRTTVLDYRVEYVRGEYVIYGLYEGRWHEVFPLIEPYQIGEQFEYQ
                     GTRAEEIAYRYNAQLFNTTAITPRFWKVLELKRNGDELLIRVETRVGALKYTALSRYA
                     FTLDKAGNRVLRFSIEELGSNSWAIFSSPNEGEEGVFVASAIR"
     gene            8802..11321
                     /gene="topA"
                     /locus_tag="PLF67_05045"
     CDS             8802..11321
                     /gene="topA"
                     /locus_tag="PLF67_05045"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOE89413.1"
                     /translation="MSEKNTLIIVESPTKAKTITKFLPKTYKVVASNGHVRDLPEERM
                     AIKIEKGFAAEYEPVKGKQRLIADLKKELKDADRLLLATDEDREGESISWHLLEVLKP
                     KIPYRRMVFHEITKKAITNALETGRDLDENLVHAQEGRRLVDRLYGYTLSPTLWKKLS
                     NKKLSAGRVQSVGLRLTVDRERMRIAFEQNTYWDASAELTNAKGELFEAKLVSYQDRA
                     IASSRDFDATTGAYKGKKGTLHLGEEEARAIVKELKEEQFVVTSIERKPFITRPSIPF
                     TTSTLQQDATRKLRISASEVMRIAQRLYESGYITYMRTDSPSLSREGTMAARTQVEEL
                     YGTEYLSPKARYFAAKSAQAQEAHEAIRPAGDAFVHPQDTRLSGRELALYDLIWKRTL
                     ACQMADAQKATTTATIEAGAGIFTATGTEIVFPGFIRAYVEEVEDNEIDKDKILPPLG
                     EGEVCPLKSLTAVEHQTRLPARYTEASLVQELEKRGIGRPSTYATIIKTLLDRKYVVK
                     EGSALVPTFIGFGVCQFLETNFKQFIDYDFTVRMEEDLDKIARGELKKETFLAEFFLG
                     EHGLMEQNRQQLTLDNKGESKTLNLCHIDEANPIMIGPYGPYVVLKDGQLVSLPYEWT
                     PGSITDADVKALIANGKQQPEAKTLGPGPNTGEPIQLLSGRFGPYWQEGEGKDAKRAS
                     VPKWVQDGDLQEDGAIATRYLSLPRILGKDKEGMEVVATKGKYGPYIASGDKTRKLNR
                     LDKDKQLFDITLEEALELLGKEEVKGSKKRGVRTASAPLKELGEFEGRKITLHNGRYG
                     FYLKHGTSNITLPNEYKKDEEKALSLRPEEAQRIVEEKQST"
     gene            11412..12401
                     /locus_tag="PLF67_05050"
     CDS             11412..12401
                     /locus_tag="PLF67_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89414.1"
                     /translation="MLQGVGINPSSLQRDDESQAFYREVVASRLNIFTSYGSGDEEYL
                     QTLDEQIRVFDEIYVDTAPIIQKDWFLHFVADSKDILKRRKKRLIILEKTMEELYGLK
                     SNPEKDQDVRIRAIIRPDLIRSLAKQRIVRLGDTGSGGIADDHLVELFRRIGANLNLM
                     LITQDRGLSERIVHLATELGEECEEVKPLTFLEKLIGKKEREVITHRMIACKLIEGGK
                     LKRLYICPECSQSYYDDLYATEGIVLCGRCYIRLREEEERKELANRKRREAQLKAEEE
                     RQKKLEAEEERLERERNRLTVEKLIQKRKQLAIFYSSIGMTILLLIIALLVSR"
     gene            complement(12429..12502)
                     /locus_tag="PLF67_05055"
     tRNA            complement(12429..12502)
                     /locus_tag="PLF67_05055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12466..12468),aa:Arg,seq:cct)
     gene            complement(12549..13010)
                     /gene="mscL"
                     /locus_tag="PLF67_05060"
     CDS             complement(12549..13010)
                     /gene="mscL"
                     /locus_tag="PLF67_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOE89415.1"
                     /translation="MAQKKGSLLEEFKKFISRGNVMDMAVGIIIGTAFTAIVSSLVKD
                     ILMPFISLIIGGINFTDLKIVIAQATADTAEVAITYGTFIQKVIDFLIIAFVVFMMVR
                     TLNLLQARLKKQEEQEEQEEQAVVAEKKPAPPAPEIVLLTEIRDLLKKQAD"
     gene            13213..14382
                     /locus_tag="PLF67_05065"
     CDS             13213..14382
                     /locus_tag="PLF67_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89416.1"
                     /translation="MKKSLLITVALVALISVSVFAGGSGETGPKIGFIGPMTGDNANY
                     GILCNNSVILAVEQFNAKGGIDGKLKVTLVTEDSEGVPEKGLSAIEKLSSSDKISGLI
                     GPVFTGVAFAVGERVQNEGIVMLSPSATHADITSIGDYVFRTVVSDGLQGEVAGHYFY
                     EKLGYRTIGVLYAKNDYSQGLFEGMTASFEKAGGKMIISEAFQVGDKDFKTQLTKIRS
                     ANPEAIYIPNYTAEMAQILEQASQLGIKIPFVSCDGFSNPDIYNLAGDFTHGVVYVGP
                     AQVAESQAYSKFKSDYEKKFGVSPDSFATNAFDGANILLAAMEKVYKATGKFDRKAIR
                     DAVAATKDYQGVTGTVNFAENGDLVAYQGLYRVNGTTPEYIGTFTVVDGVLTQVD"
     gene            14479..15396
                     /locus_tag="PLF67_05070"
     CDS             14479..15396
                     /locus_tag="PLF67_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE89417.1"
                     /translation="MGIAEFFQHLTNGLTLGGIYALIALGYTMVYGILKFINFAHGDV
                     LMVGSYAGLFIYNALRGDASLGPWVLIAFFLAMLASMVISALLGVTIERVAYKPLRKA
                     TRLAPLLSAIGVSFILSNSAAWAFGTNSKKFHYPFDNTPFVFKGVVITPHQILILSVS
                     LVMMVILKIFVDRTRMGKAMRATSLDQQTAMLMGINVDRVISLTFSIGSALAAVGGIL
                     IALDFKVYATMGTMTGLKAFVAAVFGGIGNITGAMVGGILLGILETFGVAIFGIPSGL
                     KDTIAFGVLIIILLVKPAGLLGKDEKEKV"
     gene            15398..16303
                     /locus_tag="PLF67_05075"
     CDS             15398..16303
                     /locus_tag="PLF67_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE89418.1"
                     /translation="MLNFFLLILIYSGIYALMALGQNVITGYGGMLSLTQAGFFAIGS
                     YATAILSTRFGFSFWATLPPAAILSALCGLLIGLPTLRLKGDYLAIATLGFGEIVRNV
                     LNNWDSLTRGPMGIQKIPMPTIFGFVINPYTKYSFLVMVVVFVAIAYVLFQRLARSRM
                     GRALAAIREDEIAAEAMGINITKYKVYAFILGASVAGIAGSLQAVFTLSVTPGTYTFM
                     VSVMVLCMVVLGGMGNFKASILGAFIIQLISYFPQLTGLTSVIPPQFKQILFGLILVV
                     MMIWRPQGLLGREEIRYGNEEGEKA"
     gene            16300..17076
                     /locus_tag="PLF67_05080"
     CDS             16300..17076
                     /locus_tag="PLF67_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89419.1"
                     /translation="MTILETERLTRAYGGVIAVNNVDFQVEGGLITGLIGPNGAGKTT
                     LFNNMTGLDTPSSGKVRFMGKDITGYPAHKICRLGIARTFQNIRLFKDLTVAENVMIG
                     RHFKTGKDETKGRFLNALKSYIHLKEEENAIYDKALQWLDFFDMHSYRSELARNLPYG
                     KQRELEIARALATDPQLLFLDEPAAGMNPQETEHLIRIIRKIRELGITVVLIEHDMRL
                     VMNICDSITVLNYGEKLAEGTPTEIKNNPLVIEAYLGRDE"
     gene            17076..17810
                     /locus_tag="PLF67_05085"
     CDS             17076..17810
                     /locus_tag="PLF67_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89420.1"
                     /translation="MKELLTVDTISVSYGNIRALKEVTMHVYEGDIVCLIGANGAGKS
                     TLLKAIVGLEPLVEGTITFDGEEICTANRGRQMTTDLIASRGIALVPEGRRVFADMTV
                     EENLDMGAFLVRDDALIAQRKEAMYDFFPILGARKRQKTRSLSGGEQQMMAIARALMS
                     GPRMILLDEPGLGLAPLVVADIFEKIDVINKQDGVTVFLVEQNARMALRAAKEGYVME
                     NGRIVLYDKAANLLEDEKVRKAYLGE"
     gene            17867..18511
                     /locus_tag="PLF67_05090"
     CDS             17867..18511
                     /locus_tag="PLF67_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE89421.1"
                     /translation="MIIERRMSRNPVVASPDMSISEASALMKQEKVHRLPVLDKDKKL
                     VGIITEKDILYATPSPMSSLSIHEMAYLLSKLTVKKLMSKNVVTITKDTTVEEAARLM
                     VDQDLSSLPVMEDDKLIGIVTKSDMFKILLELFGARHFGVRISFIVEDKPGTIAKISQ
                     ALSEAGIDIITFGTFMGTDPTNAICTIKVQGAPMSEIVEIVKPFVIQLLDVREV"
     gene            18511..19368
                     /locus_tag="PLF67_05095"
     CDS             18511..19368
                     /locus_tag="PLF67_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOE89422.1"
                     /translation="MAKKKQSRPVAKDSQHESPSARKEPVVLGYDPSADFGDILESWE
                     RSGTLEGVTKRMKRSESVEFSRSFGEILDEWENKGKKPKKKKTPEPMKKSAPYKPTKD
                     FGALLDEFEGAQKPKIPKTAKQSKKRHEPKKLVASVEVEEALQDRNESSATWSFADTY
                     RKWNEISDEEGALKKAVKEKAKKPRSEEPTLSALRTMLPEVVLDLHGMTVSEAEREAR
                     AFLATALEHKIRKLAFIVGKGLHNEAGYSLIREEVERILRLSRNVREFFSPPARYGGS
                     GVIWVILKG"
     gene            complement(19413..19631)
                     /gene="infA"
                     /locus_tag="PLF67_05100"
     CDS             complement(19413..19631)
                     /gene="infA"
                     /locus_tag="PLF67_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOE89423.1"
                     /translation="MAKEEAIEVEGIVREALPNTMFRVELQNNHVILAHLSGKMRKHY
                     IRIVPGDSVRVALSPYDLTKGRIIYRER"
     gene            complement(19692..21077)
                     /locus_tag="PLF67_05105"
     CDS             complement(19692..21077)
                     /locus_tag="PLF67_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOE89424.1"
                     /translation="MSNSQKREVLGSRLGFILLSAGCAIGLGNVWRFPFITGRYGGAA
                     FVLIYLLFLVILGLPVMVMELAIGRASKQNIGLSLKTLTPEKKGWHWYGHVAIAGNYV
                     LMSFYVMVSGWVLSYLFKTLGGTFVGLDAAGVGGVFGEMLGKPGNMIFWGVLAIILGM
                     IPVGIGLQKGVEQITKYMMVGLLLLLIILAGNSIALKGGGEGLKFYLVPNFSRMAEAG
                     VGNAIYAAMNQAFFTLSLGIGSISIFGSYTSDKHRLTGEAIRIISLDTFVALLAGLII
                     FPAAFAFNVEAGAGPSLLFITLPNIFNQMRGGLFWGTLFFLFMSFAALSTVIAVFENI
                     VSYWIDVHGVSRRKAAIYNGLAMVGLSLPAILGFNVLSSFQPLGPGSGILDLEDFIIS
                     SNILPIGAFIFSIYTSWRYGWGWDNFIAEADKGTEGAEFPKILKPYFQYVLPLIIFVL
                     WILGYVDIFGA"
     gene            21246..22781
                     /locus_tag="PLF67_05110"
     CDS             21246..22781
                     /locus_tag="PLF67_05110"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine hydroxymethyltransferase"
                     /protein_id="HOE89425.1"
                     /translation="MRQTSALGKYIQAYGTENEAMIGFVANLDLIAKVDDQIAARIVN
                     ELSDQRTHLKLIASENFSSLSSQAAMGNLLTDKYSEGFAYHRFYAGCDNVDAIEERGA
                     TYAKELFGAQHAYVQPHAGADANLVAYWAILNTKVTVPSLEEIGISNPADLSREDWNR
                     IRHAVGNQKLLGLDYYSGGHLTHGYRQNISAQMFDAYSYCVDKETGLLDYDAIERQAL
                     ELKPLILLAGYSAYPRAINFKRLSEIAKKAGSVFMVDMAHFAGLVAGKVFTGEYDPVA
                     WADVVTTTTHKTLRGPRGGLVLCTEEYAESVDKGCPLVLGGPLPHVMAAKAVALKEAL
                     DPSFADYAHKIVENARALAQACIDEGITVATGGTDNHLMLLDVRSFGLNGRQAESALR
                     EAGMTLNRNSLPFDPNGPWYTSGLRIGTPALTTLGMGKREMKEVASIIALVLKNTKPV
                     ILTKGENAGQPSKARAVTEPGAREEAKERVLALLGRFVLYPQLDLQFLQTAFPLDKQG
                     VEW"
     gene            22809..23192
                     /gene="gcvH"
                     /locus_tag="PLF67_05115"
     CDS             22809..23192
                     /gene="gcvH"
                     /locus_tag="PLF67_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOE89426.1"
                     /translation="MSRILDSLRYSKDHEWVRIEGEKAYVGITDHAQDELGEIVFVEL
                     PPIGERYKKDEEISSIESVKAASPIINPIEGVVLEANEDLDGSPELINEDCYANHLYV
                     LGSFSQEDYDSMMDSTEYASYLASL"
     gene            23212..24561
                     /gene="gcvPA"
                     /locus_tag="PLF67_05120"
     CDS             23212..24561
                     /gene="gcvPA"
                     /locus_tag="PLF67_05120"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOE89427.1"
                     /translation="MIYPYIPHTDEDRAQMLEALGLSSIDRLFEVVGEDLLLEDSVPI
                     AHSRTEDEVQRMIDSIAQRNIRGISFLGGGCYDHIIPSTVKALSSLPSFVTAYTPYQA
                     EMSQGLLQAIFEFQSMVCEITSMDIANASLYDGSHAIVETALLMVNAKRGAKKVLVSE
                     TIHPFALKALGTWVLGTEYEVVPIQEKDGVVDLSNLVIGDDVGGLIVQSPNRYGFVED
                     YSGIADLLHERKALFAISSDPLSLAIQKTPGQWGADIAVGDTQSLGLSLSFGGPSCGY
                     MAVKQPLLRRIPGRLVGITTDAEGKRGFTLTLQAREQHIKRERATSNICSNQALAALM
                     TTIHLSSLGWHGMVEAARQSYAKAHYLAHHLSQLPGVEVMNAHPFWCEFPVVFHDAKR
                     MRKFLQELRNEGIFAGVRLSALTRKDTDELVLLVAVTEKRSREELEMYIAAAQRVMK"
     gene            24558..>25945
                     /gene="gcvPB"
                     /locus_tag="PLF67_05125"
     CDS             24558..>25945
                     /gene="gcvPB"
                     /locus_tag="PLF67_05125"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOE89428.1"
                     /translation="MIEPLLIDKSKKGRLAYCYPSLDLPRSFDQELAHLPSHLQREGS
                     ARLPEVSELDVVRHFTRLSRMVHGVDNGMYPLGSCTMKYNPKLGEEIARMAQFTEIHP
                     LQDISTVQGCLSVMYNLLEDLSSITGMSWGSLQPCAGSHGEYTGLKMIRSYFLKKGDV
                     GRTKVLIPVSAHGTNPASAAVNGFDVVEIASNRKGLVDLADLKSKLSDEVAALMLTNP
                     NTLGLFEEDILAISEAVHEAGALLYYDGANLNAIMGKTRPGDMGFDVLHLNLHKTFST
                     PHGGGGPGSGPVMVNDLLRPFLPKPDIDLREGGYVFDWDRPDSIGKVSLFWGNFLVFL
                     RAYVYILRMGSEGLRAAANHAVLNANYLAKKLAPYYEIPHTGGIMHEFVISVKNYKEQ
                     YGVTAEDFAKALIDEGYHPPTVYFPLIVDEALMIEPTESESLESLDAFIAAMIRIAEK
                     AKSDPASLKKAPV"
BASE COUNT         6122 a         6901 c         7450 g         5472 t
ORIGIN      
        1 cattaaacca caactttccc cctatggaat aaatcatttt cgattactct cggaattctt
       61 atattcgagg catccagctt gaactcttcg attgagccat ccttgacagc ggagcgacag
      121 ctttactact atctctgtat tatgaaaaaa cagcttaccg ccaacgaatt acgccaaaaa
      181 ttcatcgact tttttgtttc caaggaccac aaacagatca gtggcgcctc gctcattccc
      241 gagaacgacc ccaccgttct cttcaccacc gcgggtatgc acccgctcgt tccctatatc
      301 ctcggcagcg agcacccaag cggaaggcgc ttggtgaact atcagaaatg catccgcacc
      361 ggtgatatcg aggcggtcgg cgatcctcat cacctgacgt tcttcgagat gctcggcaac
      421 tggtcgctgg gggattactt caaagaggag gcgatcgcct acagccatga gtttctaacc
      481 accgtcctgg gattggaaac ggacgagctt tcggtaacgg tcttcgccgg agatgatgaa
      541 gtgccgcggg atgaagaagc cgctgcagcg tggcggaagc acggggttcc cgatgagcgg
      601 atctacttcc ttccccggga ggacaactgg tggggtcccg ccggtgaaac cggaccctgc
      661 ggaccggaca gcgagatgtt catcgacacc ggacgggatg catgcggccc cgattgtcgg
      721 ccgggctgtt cctgcggcaa gtatttcgag atctggaacg acgtgttcat gcagtatgtg
      781 aagaacgccg acggcaccta cacggaaatg gagcgcaagt gcatcgatac cgggatgggg
      841 atcgagcgga cgattgcgat ccttcaaggg aaacgatcgg tatatgagac cgaggtgttc
      901 accccgatca tcaagcggat cgaaactctg gcgaacaaaa cctacggtga aaacgaagag
      961 gacgacatgt cgatccggat tctctccgac catgtccgaa ccggcgtctt catcctcggc
     1021 gaccagaaag gtgtttcccc ctccaacgtc ggtcagggat acatccttcg ccgcctgatt
     1081 cgccgggcgg tccggcacgc ccacaagctc ggcatcgaag gttctttcct ttccgagttg
     1141 gccgaggtcg ttatcgagct ctacaaaaaa ccctatcctg agcttcttga aaaccgggag
     1201 ttcgtgatga aggagcttgt cgccgaagaa gcaaagttca gtgcgacctt ggcgaaagga
     1261 gagcgggagt tcgaaaagat gcttccgaac ctgcttaaga gtccgaaccg gatcatcagc
     1321 ggtcggactg cgttcaagct ctacgatacc tacggctatc cgatcgagct gaccagggag
     1381 cttgccgccg aaaatggctt tgaagtcgac gaaaaggggt ttcatgaggc gtttgccaag
     1441 catcaggaac tcagtcgcgc cggcggcgac cagacgttca aaggcggatt ggcggatcac
     1501 agcgagcgga ccaccgccct gcatactgca acccacctcc ttcatcaggc gctgaggacg
     1561 gtcctgggcg atcatgtcgg tcagaaggga tcgaatatta cggtcgagcg gctccggttc
     1621 gattttaccc accctgagcc gatgactcag gagcagcttc aaaaggtcga agctatcgtc
     1681 aacgaggtca tcaggcggga tcttccggtg agctttacca cgatgagtct ggaggaagcc
     1741 cggcagtcgg gcgcgatcgc cctctttgaa ggccgctacg acgagcaggt aaaagtctac
     1801 tccgtcggag acttctccaa ggaagtctgc ggaggacccc acgttgagcg gaccggcgga
     1861 atgggaacat tcaagatcca gaaagagcaa tcatcctcgg cgggagtccg ccggattcgg
     1921 gcaaccctcg aataaccgtt tcattggctg tcgggcagtg tccggcagcc aattattttt
     1981 cttcatccgc attcttatct tttggtttca gggttgtggg aagtggccag ttgtccgcac
     2041 gctccgccga tcgtccggcc gcgggagatt cgaagggtgt gtttgacccg atagcggttg
     2101 agaagggcgg agaaggcttt ctgctcctct tcgctcggag tggagaactc aagctcgtcg
     2161 atcgggttcc aggcgatgag gttcacgatg acatccagac cgcgggtaaa gagctcgacc
     2221 tgtctggcgc tttcacctgt ggtgttcacg ccggacagca tgcagtattc aagggtgagt
     2281 cgtttaggta ccgattcctg ataggcaaga agcgcttcct tgagctcggc gagcggccat
     2341 cgcttgttga ccggcatcag gcgggttctg agcgagtcat ccgcgctgac cagtgaaacg
     2401 gcgagcttga tcgggagctt caattcggcg agcttgttga ttccgggaac cactccggat
     2461 gtgctgatcg tcattcttct gaggccgatg ttgaacgtct ctttttgatt gaggtagtgg
     2521 atcgccctga tcgtcggatc gaagttgtga aacggttcgc ccatccccat gaagacgatg
     2581 tgggtgatcg gccggcttgc cgccttggaa aggtggacgt actgttcgat gatctcttcg
     2641 gaagcaaggt tacgaacgag gcccatcgtt ccggtcttgc agaaggtgca ccccatcgca
     2701 catcccacct ggacagagag acacgcggtg tgccgtcctt tacggtcgac gagcaggacc
     2761 gcttcgatca tttttccgtc gtgaagcgtg attccgagct tgatcgttcc gctttcatcc
     2821 tcctctcgga ccgttgtctc cgatgagatc ggactcccga cgaggtcgct taggcgcttt
     2881 cgctcatcga ggggaaggtc ggtcatctcg tcaaacgtag tggctccctt gccgagccag
     2941 gcaaggatct gcccgccata gcgtttctcg gggagggaca gcgattcgac gaggcgttcg
     3001 gaagtgagac cgtagaggga aaggggaaga ttcatggatt cagtatacct atttgagcat
     3061 tgaaagaata gcgatcagca cgccggtgat tccttctccg ccaagcagtc cgctggcgat
     3121 caatccgatc tttttctggg atttctcctt gtctttcttt cgggtgaaga tcaaagaggc
     3181 gagtgcgcca aggcccatga tcgaagagat gtagatcggc agatacacgc cgaggcccag
     3241 ggtggcggag ggtattttca tcagaaagag cgccaacccg acgaagaggc cgatcaggaa
     3301 ggccggcagg tgttcgattc cgccgaccat cttggcgacc gccgctgcct gaggggcggg
     3361 cagcagggcg gtgccgaagc cgccgaacgc atgcttcatc gccaagagga cgatgaccga
     3421 gacaaccgcc ccgatcaccc ctccgattcc ttcaccgagg atctgatccc ggggattggt
     3481 tccgaggagg tgtccgcttt ttaggtcatt catcacgtcc ccggtcaagc cgcaggcgac
     3541 ggcgaccacc gccgcgatcg agaaggaggc gagcagggaa ggcttggaga tcagctggat
     3601 caccagcagc acgaggatgc cgaagatctc catcgggttg atgcccgtct gtccggtgag
     3661 catcccggag aggtaggtcg tcagatagat gccaacgatg agcaccagcg cctcgacaaa
     3721 gcccagctcc gtccccagtg aaagaagaac caccgccagg agcaggacaa gcgatatgag
     3781 aaagagcgtt cgttgactct ttttcggttt ctcttccaac tttttcggtc gaagaatctt
     3841 tgaaaagagg gcgtggataa agacgcccaa ccccgttccg atcatcagcc cgatgccgag
     3901 gttttgcctg aagacatccg cagcggatat gtcgccgaaa agaccggcct tgattccgat
     3961 cggagtgagg agatagtagc cgaagatcgc tccgccgaac cagaaaaggg cgaagaccgg
     4021 gccgatgatc gccccgattc ccgcggccat cggcgagacc cagaaggaga aggaaggaaa
     4081 gagagcgctt cctttgaagg gggtaagcaa agccggtatc tttgcccaca cgtcccttag
     4141 gagggtgaag agaaccgatc ctcccattga cgtaaagagc gtcttcgcac cgctgcctcc
     4201 ttcttcggaa gccttgaggg tgttgaacgc ggcgatgccc atcggatagg gcagctcctc
     4261 ctcaacaatc aggcgttttc tgaggagggc ggagaaaagg gtgcccagaa tcgctccgac
     4321 cacggtgagg gtgatgaggc tcatcggcgg gaacacggca tcaggattga gcatccacaa
     4381 gccgggaatc gtgaaggcga gtccaccggc gaccatcgcc ccgctgctca tgatcgtatg
     4441 ggtgacattc atctcttgca gggaggagtt gcgtgctttt ttcaatgcgg ccatcgaaag
     4501 aacggtgacg aagatcgtcg gccacggaag ggcgcccatt ctgagggcga catacattga
     4561 ggatgcggta atgaccagca atcccaagat tccaatgatg attgcacgtg cggtgaggtg
     4621 tcgttccata gatacacttc ctccatatgt gtaatagtaa cgagaaagga tagcgggtgg
     4681 aagagtcaga ctcggtagga attcagcgag gaagcgaagc gttcggcgag gcgggctttt
     4741 acgatgatcc cctcttcggt ataggagatg tccgctacct gtccgctccg atggagatat
     4801 gccacgaggt catgacggtc gttggggaac ttgtaggaaa gggtcggatt cagggcgtgg
     4861 atcgtctcgg tgatcgcatc gatcagcgtc tccaatccct gaccgctctt cagggatgtt
     4921 tccaggaccg gttggtagag gctcttgagg cgggaaagcg cgaagaagtc ctcgaccttg
     4981 tcgatcttgt tggccacaac gatcgcaggc ttgtcggtaa cgccgagatc ttccaaaacc
     5041 tgaaccgtgg tggtgtaggt ggcgatcatg tcgggatgac ttgcatcaca gacgatgatg
     5101 agaaaatccg cataccgggc ttcctccaac gtggatttga acgcatcgac cagatggtgg
     5161 ggcagatcgc tgataaagcc gaccgtgtcg cttaagagca cttcttcccc tccggggagc
     5221 ttgaagaggc gggtcgtggg atccaaggtc gcaaaaagct tgtcctcagc aagaactccc
     5281 gcttcagtga gggtgttgag cagcgttgat tttccgctgt tcgtatagcc cacgatcgct
     5341 ccgatcggat tggggctctt taagcgggag gagcgttgca cctcccgctg ttggactacg
     5401 ttgagaagct gcttcttcaa aagggcgatc cggtcctgaa tccggcgtcg gtccagttcg
     5461 agccgggttt caccttcgcc tcgggatccc cgaactcctc cgcgttgctg tgaaaggttc
     5521 gtccacttgc gaaccaggcg gggaagggag tattggagcc gggcgagttc gacttgcagc
     5581 atcgcctcct ttgtctgggc gcgatcggcg aagatctgca gaatcacctc gtcgcggtcg
     5641 atcaccgcgg attggaagat ctcttccaag ttgcgctgga cccgcgggga gatcgggcgg
     5701 tcgaagatga cgacatccgc ttcaagctct tcaagcagct ccttgatttc agccactttt
     5761 ccactgccga tgagggtggc gctggtttct cctcgaaggg ggatgaactc ggtgtgaaga
     5821 acctccaagc ccatcgtgtc aaccaacgag gcgagctctt cactgcgcag atcggcattt
     5881 tggcgggtct cgttttcatc aatgagaatc aataacaggg cggagagcat ctcgtgctca
     5941 agggaatgca ggggtttact cacgccctga ctatagggcg tggttaacga gctggtcaat
     6001 atgtggtaca cttcttccgt ttgaagaatc agaggttgcc atgggactta attgcggtat
     6061 cgtcggcttg ccgaacgttg gaaaatcgac tcttttttca gcactcacca gtgctccggc
     6121 ccacgtggcg aactatccgt tttgcacgat cgatccgaac gtcggaatcg tcgcggttcc
     6181 cgattcccgc cttgatcgga tcgcaaaact gatccccggt aaaaagaaga ttcccgccac
     6241 cgttgaattc atcgatatcg ccggcctggt cgccggcgcg agccgggggg aagggttggg
     6301 gaaccgtttt ctggcctcga tccgggaggt gggcgtgatc gcccacgtgg tccgctgctt
     6361 cgaggacggc gacgtcgtcc acgtcaacgg caacgtcgat cccgagcggg atatcgaaac
     6421 aatcaacatc gagctggccc tcgccgatct ggacacggtg accaagcgct acgagaaggt
     6481 gtccaaaggg cgctcgcttt ccaaagaagc gcaaaaacat gcttcagaga ttcttccgat
     6541 ccttgaacga ctccttgcga cgctgggcga gggcggaagc gcccgaagcc tgaagcttga
     6601 cgaagatcag ctttccttga tctccgacct tcatctgctc accgtgaagc cggtgatcta
     6661 tgtctgcaac gtggatgaag agggaatcga cgggagctcc gcccatgtgg atgccgtcaa
     6721 acggattgcc ggggaggagg acgcggaggt ggtggtcatc tgttccaaga tcgaaagcga
     6781 gatcgcgacc ttggaaacgg aggaggagaa acaggagttt ctggaagcgg tcggattgaa
     6841 ggaatcgggc ttgaacaaat tgatccggag cgcgtattac attctgggtc ttcgaacgtt
     6901 cttcaccacc ggtgacgatg aaaaccgggc ttggacgttc aaggcgggaa tgaaggcccc
     6961 ccagaccgcc ggtctcatcc acagcgattt tcagaagggg ttcatcaaag ccgaaatcta
     7021 cagctgcgac gacctgttcg cctacggaag cgaacagaag ctgagggtcg ccggaaagct
     7081 ccgccttgag ggcaaggagt accttgtccg ggacggggat gtgattcatt tcaaattcaa
     7141 cgtgtaaacg gtctgctacc acgtcaccgt gaaggtgatc ggttcggcga gaagcaggaa
     7201 atcgatcaag gggaaactga actcgtaggt cgtcggatcg agctttcttc cgtttatcat
     7261 gcttttgatc ggcttgggag tcgtgacgcg cagggtgatc gtcgactgtt caatcgccgg
     7321 cggcccttct tcgccgagca tgaagctgat catctccaga tagtcgtcgg cgctgaggcc
     7381 ctgattgtac accgggccga acgcctcgaa gttctcatcc gccaggaagg ggatgatcgg
     7441 aacgagctgg ggataggttt ccaaggtcag gtagaaggtg atgctgtcgt ttgtcgcttt
     7501 cagaagctgt tggtctttcg gagctccgag atcgcgtacc agattctcca catcggaaaa
     7561 gatgaaggtg ccgttccacg cgttcacgcc ggtcttatcc agctgaaccg cctttgtcga
     7621 gggggcgctg tcgagaagcg tttcaaagtc atcgatcgcc tgatccatga cggggcgatc
     7681 gctttttttt gtgctgaagt cgctgaagtc ttccaggaca tcgacgaaaa actcctctac
     7741 gttgaacgtg aaatccgctt tcgggggggc gggagagaag tcgaggcgtt gactgaccgt
     7801 acagccggcg agaagaaaca cgaggatgag gattccgatc caatgaatgg ttttcatacg
     7861 cattacctta cgagatttat caagagttct caactgcaaa atacatctat ccacccctat
     7921 cgttcctgaa aaaaaacggc tatcctatag atgatgatga gacgaatgct attttttctg
     7981 atgatcatcc tgatcgggag cgccctccat gccctggatc tccatgtcga cgagatccag
     8041 ccgagccgga tcactctttc ctgggatcgg gttccctccg ccctcaccta tgacctgtac
     8101 ctgaacggct gctaccgggc gacggtgaga accccctcat acagcattga gcacctcact
     8161 tcccatactt cgtacagcgt cagagtccaa gccaaaggtc aaggaacggt catcgacgaa
     8221 gggacgatcg aggtgaccac ctccggttgg gaggggcggt atcagtgggt caatagaacg
     8281 cgaaaggata atcgcgggcg gacgaccgtt ctggattacc gggtcgagta cgtccggggc
     8341 gaatatgtca tctacggtct ctacgaagga cgttggcacg aggtgttccc ccttatagaa
     8401 ccatatcaga tcggagaaca gttcgaatac caaggcactc gagccgaaga gatcgcctat
     8461 cggtacaacg cccaactatt caacaccacg gcgattactc cccgtttttg gaaagtcctc
     8521 gagctgaaaa ggaacggcga tgagttgctc attcgggtgg agacccgggt cggagcgctg
     8581 aaatacacag cgctcagccg ctatgcattc acccttgata aagcgggaaa ccgggttctc
     8641 aggttctcga tcgaagagct gggttccaac agctgggcga tcttttcttc acccaacgag
     8701 ggtgaagaag gtgtctttgt cgcttcggcc atccgttgac aaaaaagaag aggaccagta
     8761 taccttggga actgaggcct gtgcttcagg agtatacgta catgagcgaa aagaacaccc
     8821 taatcatcgt cgagtctccg acaaaagcga aaacgatcac gaaattcctt cccaaaacct
     8881 acaaggtcgt agcctccaac gggcacgtac gcgatcttcc ggaggagcgg atggcgatca
     8941 agatcgagaa gggcttcgcc gccgagtatg agccggtcaa aggcaaacag cgtctgattg
     9001 ccgatctgaa gaaggaactg aaagatgccg atcggcttct tctggcgacg gatgaagacc
     9061 gcgagggtga atcgatctcc tggcaccttc ttgaagtgtt gaaaccgaag attccctacc
     9121 ggcggatggt gttccatgag atcacgaaga aggcgatcac caacgccttg gaaacggggc
     9181 gggacttgga cgagaatctg gtgcacgccc aggaaggaag gcgcctcgtc gatcggctct
     9241 acgggtatac cctttccccc acgctgtgga agaaactctc caacaagaag ctttccgccg
     9301 gccgggtgca gtcggtgggt ttgcgcctta ccgtggatcg ggagcgcatg agaatcgcct
     9361 tcgaacaaaa cacctactgg gatgcaagcg ccgagctgac caatgccaaa ggcgagctct
     9421 ttgaggcgaa actcgtctcc tatcaggacc gggcgatcgc ttcctccaga gatttcgatg
     9481 caacgaccgg agcctataaa gggaagaagg ggacgcttca tctcggagaa gaggaagccc
     9541 gggcgatcgt caaagagttg aaagaagagc agtttgtcgt cacctcgatc gaacgcaagc
     9601 cgttcatcac ccgtccctcc attcccttca ccaccagcac ccttcagcag gatgcgacaa
     9661 gaaaactcag gatcagcgct tcggaggtaa tgcgaatcgc ccagcggctc tatgaaagcg
     9721 ggtatatcac ctatatgcga accgacagcc cctcgctcag ccgggagggg acgatggcgg
     9781 cccgaactca ggtggaggag ctctacggaa cggagtatct cagccccaag gctcgatact
     9841 ttgccgcaaa aagcgctcag gcgcaggaag cccatgaggc gatccgtccc gccggcgatg
     9901 cctttgttca tccgcaggat actcgtttaa gcgggcggga gctggccctc tatgacctga
     9961 tctggaagcg gacgctggca tgtcagatgg cggacgctca aaaggcgaca accacggcga
    10021 cgattgaagc gggcgcggga atctttaccg caaccggaac ggagatcgtc tttccgggtt
    10081 ttatccgcgc ctatgtcgaa gaagtggaag acaacgaaat cgacaaggac aagattctcc
    10141 ctccgctcgg tgaaggcgag gtctgtccgc tgaagagctt gactgcggtg gaacaccaaa
    10201 cccgcctgcc ggcccggtat acggaagcat cgctcgtcca ggagcttgaa aagcgaggga
    10261 tcggccggcc ttcaacgtat gcgacgatca ttaagacctt gctcgatcgc aagtacgtgg
    10321 tcaaagaggg aagtgcactc gttccgactt tcatcggctt cggggtgtgc caattccttg
    10381 aaacgaattt caaacagttc atcgactacg acttcacagt ccggatggag gaggaccttg
    10441 ataaaatagc ccggggcgaa ctgaagaaag aaacattcct ggcggagttt tttctcggtg
    10501 aacacggatt gatggagcag aacagacagc agctgacttt ggacaacaag ggcgagtcaa
    10561 aaacattgaa tctctgtcac atcgatgagg cgaaccccat tatgatcggt ccctacggtc
    10621 cgtacgtggt tcttaaagac ggacagcttg tcagcttgcc gtatgagtgg acccccggct
    10681 cgatcaccga tgccgacgtg aaagccctga tcgccaacgg caaacaacaa cctgaagcaa
    10741 agaccctcgg tcccggtccg aataccggcg agccgattca actgctgagc ggtcgcttcg
    10801 gcccctattg gcaggaaggg gaaggcaagg atgccaaacg ggcatcagta cccaagtggg
    10861 ttcaagatgg cgacttgcaa gaggacggcg ccatcgcaac ccgctatttg agcttgccga
    10921 gaatcctcgg caaagacaaa gaggggatgg aagtggttgc aacgaagggc aagtacggtc
    10981 cgtatatcgc cagtggcgac aagacacgca agctcaaccg tttggacaaa gacaagcagc
    11041 tcttcgacat caccttggag gaagcccttg agcttcttgg aaaagaggag gtaaaggggt
    11101 cgaagaaacg aggagttcgc accgcttccg cacctttgaa agagctcggt gagttcgaag
    11161 gccggaagat taccctccac aacggacgat atggcttcta cctgaagcac ggcacgagca
    11221 atatcactct gcccaatgaa tataaaaaag atgaggagaa agcgctgagt ctccgtcctg
    11281 aggaagcgca gcggattgtg gaggaaaagc aatccaccta aacgatgcaa acgcaccaac
    11341 gaaccaaaaa aacatcagcg atatcggctt tggctctcga attcagggtg agcgagcgac
    11401 gaattcgcac gatgttgcaa ggagtgggaa tcaatccttc ctctctgcag cgcgacgacg
    11461 aaagccaagc gttctatcgc gaggtggtcg cgagcagact caatatattc acttcctacg
    11521 gaagcggtga tgaggagtat cttcaaaccc tcgacgagca gatccgggtg tttgatgaga
    11581 tctacgtcga taccgccccg atcattcaga aggattggtt ccttcacttc gtcgcagaca
    11641 gcaaggatat tctcaaacgc cgcaaaaaac ggcttatcat ccttgaaaag acgatggaag
    11701 agctctatgg gctgaagagc aaccccgaaa aggaccagga tgtccgcatt cgggcgataa
    11761 tccgcccgga tctgatccgt tccttggcga agcagcgaat cgtccggctc ggcgacaccg
    11821 gcagcggtgg aatcgccgac gaccatctgg tggagctctt tcgaagaatc ggcgccaatc
    11881 tcaatctcat gttgatcact caggatcggg gcttgagcga gcggatcgtt catctggcca
    11941 ccgaacttgg ggaggaatgt gaagaagtga aaccgcttac cttcctcgaa aagctgatcg
    12001 gtaaaaaaga acgcgaggtg atcacccatc ggatgatcgc atgcaagctg atcgaagggg
    12061 ggaagctgaa gcggctgtac atctgtccgg agtgcagcca aagctattac gatgatctct
    12121 acgccaccga ggggatcgtc ctctgcgggc ggtgctacat acggcttcgc gaggaagagg
    12181 agcgcaagga actggcaaac cggaagcggc gggaagctca gctcaaagcg gaggaggaac
    12241 gccagaagaa gctggaagcg gaggaagagc ggcttgaacg ggaacgaaac cgccttaccg
    12301 ttgaaaagct catccagaag cgcaaacagc ttgcgatctt ctactcctca atcgggatga
    12361 ccatcctgtt gctcatcatc gccctgcttg tctcgagatg aagaaaggct gccggtcggc
    12421 agcctgcatc cccctggcgc gattcgaacg cacgacccct tccttaggag ggaagtgctc
    12481 tatccggctg agctacagga ggtggaacac ctcgatagta gcgaattcat cggtcatgtg
    12541 caaggttctt aatccgcctg ctttttcaag aggtcgcgaa tctcggtgag aaggacgatt
    12601 tcgggcgccg gcggggccgg ttttttttcc gcaacaaccg cctgttcctc ctgttcctcc
    12661 tgttcctcct gtttcttcag tcttgcctgc aataggttga gtgttcggac catcatgaag
    12721 accacgaatg cgataatgag gaaatcgatc accttctgga tgaatgttcc ataggtaatg
    12781 gcgacttcgg cggtgtcggc ggtcgcttga gcgatgacga tcttcagatc ggtgaaattg
    12841 attcctccga tgatcaggct gatgaacggc atgaggatat ccttgaccaa cgatgagacg
    12901 atcgcggtga acgccgtacc gatgatgata ccgacggcca tgtccatcac gtttccccgg
    12961 gagatgaact tcttgaactc ttccaacagc gaacccttct tttgggccat ataagcctcc
    13021 tctttagatc attgagccga gtatagcaca ctcgggcatc ggggaaaaag ctgtcttaaa
    13081 gcggttgatt cttatgattg tgcggaatct tgatggaatg agtgtttaaa tattgaataa
    13141 atatacgcat tattgtataa ccaaagaaat attacgttaa cgatgatgta cgcacgtatg
    13201 gaggtactaa cgatgaagaa aagcttgttg atcacggtgg cgcttgttgc attgatcagc
    13261 gtttccgtct ttgcgggtgg aagtggcgag acgggcccca agattggatt catcggtccg
    13321 atgaccggtg acaacgcgaa ctacggtatt ttatgcaaca actccgtcat tcttgccgtt
    13381 gaacagttca acgcgaaagg cggcattgat ggaaaactca aagtaaccct cgttactgaa
    13441 gactccgaag gagtgcccga gaaaggcctc tcggcgatcg agaagctttc ctcctccgac
    13501 aagatctccg gtctcatcgg accggtcttc accggggtgg cgttcgcagt cggcgagcgg
    13561 gtgcagaatg aagggatcgt gatgctcagc ccctcggcga cccacgccga catcacctcg
    13621 atcggtgact atgtgtttcg aaccgttgtt tccgacggct tgcaggggga ggtcgccggc
    13681 cactacttct atgagaagct cggctatcgc acaatcggtg ttctgtacgc aaagaacgac
    13741 tacagccagg ggctctttga agggatgacc gcttccttcg agaaagccgg tggaaagatg
    13801 atcatcagcg aggcgttcca ggtcggagac aaggatttca aaacccagct taccaagatt
    13861 cgcagcgcca accccgaggc gatctatatt cccaactaca ccgctgagat ggctcagatt
    13921 cttgagcagg cttcccagct cggaatcaag attcccttcg tctcctgtga cggcttctcc
    13981 aaccccgaca tctacaacct cgccggtgac ttcacccacg gcgtcgtcta tgtcggtccc
    14041 gcccaagttg ctgaaagcca ggcgtattcc aagttcaaga gcgattatga gaagaagttc
    14101 ggtgtaagcc ccgactcctt cgctacaaac gcgtttgacg gagcgaacat cctgctcgca
    14161 gcgatggaga aggtgtataa agccaccggc aagttcgacc gcaaggcgat cagggatgcg
    14221 gttgccgcaa cgaaggatta tcagggagtt accggaacgg tcaacttcgc cgagaatggc
    14281 gacttggttg cgtatcaggg attgtaccgg gtcaacggca cgactcccga gtatatcgga
    14341 accttcacgg tggttgacgg agtgctgact caggtggact gagctcggca aacaccaaga
    14401 taacgatgag ccggtgttga gaacaccggc tcttttatgg tactgtcaac ccagttctcg
    14461 tgatcaagga gagacgtcgt gggaattgct gaatttttcc aacacttgac gaatggcttg
    14521 accctcgggg gaatctatgc gctgatcgcc ctcggataca cgatggtcta cggcattctg
    14581 aaatttatca acttcgctca tggggatgtg ctgatggtcg gctcctatgc cggcttgttc
    14641 atctacaacg ccctgagggg ggacgcttca ctgggtccct gggtgttgat cgcgttcttc
    14701 cttgcaatgc tcgcttcgat ggtcatcagc gctctcttgg gagtgacgat cgagcgggtt
    14761 gcgtacaagc cccttcgcaa agcgacccgg ctcgcccccc tgctcagtgc aatcggagtt
    14821 tcattcattc tctccaactc cgcagcctgg gcattcggaa cgaacagcaa gaaattccac
    14881 tatcccttcg acaacacccc ctttgttttc aaaggcgtgg tcatcacccc ccaccagatt
    14941 ctgattcttt cagtatcgtt ggtgatgatg gtcatcctta agatcttcgt cgaccggacc
    15001 cggatgggca aggcgatgcg cgccaccagc ctggatcaac aaacggcgat gctgatggga
    15061 atcaacgtcg accgggtgat cagcctgacg ttttcaatcg gcagcgctct ggccgccgtc
    15121 ggaggaattc tcatcgccct cgatttcaag gtctatgcga cgatgggaac gatgaccggg
    15181 ctgaaggcgt tcgtcgccgc cgtgttcgga ggaatcggaa acatcaccgg agcgatggtc
    15241 ggaggaatcc tgctcgggat ccttgagacc ttcggagttg cgatcttcgg cattcccagc
    15301 gggctgaagg acacgatcgc cttcggggtg ctgatcatca tcctcctggt caaaccggca
    15361 ggtctcctcg gcaaggacga gaaggagaaa gtgtgatatg ctcaacttct ttttattgat
    15421 cttgatctat tccggcatct atgcattgat ggcgctcggg cagaacgtca tcaccggtta
    15481 cggggggatg ctcagtctta cacaggcggg gttcttcgct atcggctcct atgccacggc
    15541 gatcctttcc acccgcttcg ggttctcctt ctgggctacg ctgccccccg cggcgatcct
    15601 gagcgcgctg tgcggcctct tgatcgggct gccgaccctc cggctcaaag gggactattt
    15661 ggcgatcgcc accctcggct tcggggagat cgtccgcaac gtcctgaaca actgggacag
    15721 cctgacccgg ggaccgatgg gaatccagaa gattccgatg ccgacgatct tcgggttcgt
    15781 gatcaatccc tataccaagt attcgtttct ggtgatggtc gtcgtcttcg tcgcgatcgc
    15841 ctacgtgctg ttccagcggc ttgcccgttc acgaatgggg cgggcgcttg cggcgatccg
    15901 tgaggatgag atcgcagcgg aagcgatggg gatcaacatc accaagtaca aggtctatgc
    15961 gttcattctg ggggcttccg tcgcgggcat cgccgggtcg cttcaggcgg tcttcacctt
    16021 gagcgtcacc ccgggcacct atacgttcat ggtctcggtg atggtcctct gcatggtcgt
    16081 cctgggggga atgggaaact tcaaggcttc gattctcgga gcgttcatca tccagctgat
    16141 cagctacttc ccgcaactga ccggccttac cagcgtgatc ccgccgcagt tcaaacagat
    16201 tctcttcggt ctgattctgg tggtgatgat gatctggcgg cctcagggtc tgctcggccg
    16261 ggaagagatc cgctacggca acgaggaagg agagaaggca tgaccattct ggaaacggaa
    16321 cgactgactc gagcctacgg cggagtgatc gcggtcaaca acgttgattt ccaagtggaa
    16381 ggcggcctga tcaccggact catcggcccc aacggagcgg gaaaaacgac gctgttcaac
    16441 aatatgaccg gcttggacac tccttcgagc gggaaggtcc gcttcatggg taaggatatc
    16501 accggttatc ccgctcataa gatctgccgc ctcggcatcg cccggacgtt ccagaacatc
    16561 cgtctcttca aagatctcac cgttgcggag aacgtgatga tcggccgcca cttcaaaacc
    16621 ggcaaagatg aaacaaaagg gcgttttctc aacgctctga aaagctacat tcatctgaaa
    16681 gaggaagaga acgcgatcta cgacaaagcg cttcagtggc ttgatttctt tgatatgcac
    16741 tcctatcgga gcgagctggc cagaaacctg ccgtacggga agcagcggga gctggaaatc
    16801 gcaagggcgc tcgccaccga tcctcagctg ctctttctcg atgagccggc ggcgggaatg
    16861 aacccccagg aaaccgagca tttgattcga atcatcagga agatccggga gcttggaatc
    16921 accgtcgtct tgatcgaaca cgatatgagg ctggtgatga atatctgcga ctcgattacc
    16981 gtgttgaact atggagagaa gcttgccgag ggtactccga cggagatcaa gaacaatcct
    17041 ttggtgatcg aagcgtatct gggaagggac gagtgatgaa agaactgctt accgtcgata
    17101 ctatttccgt ctcctatggc aatattcgag ccctcaaaga ggtgacgatg cacgtttatg
    17161 agggagacat cgtctgtctg atcggtgcca acggagcggg aaaatccacc cttctgaagg
    17221 cgatcgtcgg actggaaccc ctcgttgaag gcacgatcac cttcgacggg gaagagatct
    17281 gcaccgccaa ccggggccgt cagatgacta ccgatctgat cgcttctcgg ggaatagccc
    17341 tcgttccgga ggggcggcgg gtgtttgccg acatgacggt ggaagagaac ttggatatgg
    17401 gggcgttcct tgtccgcgat gacgctctca tcgctcagcg caaggaagcg atgtatgatt
    17461 tcttcccgat tctcggagcg cggaagcgac aaaagacccg ctcactttcc ggaggcgaac
    17521 agcagatgat ggcgatcgcc cgggcgttga tgagcggacc gaggatgatt ctgctggatg
    17581 agccggggct cggtctggcg cctctggtgg tcgccgacat ctttgagaag atcgatgtga
    17641 ttaacaagca ggatggagta acggtctttc tggtggaaca gaacgcacgg atggcccttc
    17701 gcgctgccaa ggaggggtat gtcatggaaa acggccgaat cgtcctctac gacaaggcgg
    17761 ccaatctgct ggaagatgag aaggttcgca aggcgtatct gggcgaataa gagtagtatt
    17821 tgagcacaag ctcatgtacc atggaagtgg aggaaggaga gccgatatga ttattgaacg
    17881 aagaatgtcc cgtaatccgg tcgttgcatc accggatatg tcgatctccg aagcgtcagc
    17941 gttgatgaag caggagaagg ttcatcgact gccggtcttg gataaggata agaagcttgt
    18001 cggaatcatc accgagaaag atattctcta tgcaaccccg tcaccgatga gcagcctctc
    18061 gattcatgag atggcctatc tcttgagcaa gctcaccgtg aaaaagctga tgagcaagaa
    18121 cgtcgtgacg atcaccaaag acacaacggt cgaagaggcc gcccggctga tggtcgacca
    18181 ggatctttcc agtcttcccg tgatggaaga tgataagctc atcggcatcg tgacgaaaag
    18241 cgacatgttc aagatccttc tcgagctctt cggagcccgc catttcggtg ttcggatctc
    18301 cttcatcgtc gaagacaaac ccggaacgat cgccaagatc agccaggcgc tcagcgaagc
    18361 gggaatcgac atcatcacct tcggaacctt catgggcacc gatccgacga acgcgatctg
    18421 cacgattaaa gttcaaggtg ctccgatgag tgagatcgtc gagatcgtca agccgtttgt
    18481 catccagctt ctggatgtcc gggaagtgtg atggcaaaga agaagcaatc acgccccgtt
    18541 gcgaaggact ctcagcatga gagtccttcc gctcgcaaag agccggtcgt cctcggctat
    18601 gatccctccg ccgacttcgg ggacattctg gagagctggg agcgaagcgg gacgctggaa
    18661 ggggtgacca agcggatgaa gcgctcagag agcgtagaat tctcccgctc cttcggagag
    18721 attctggatg agtgggagaa caaaggcaaa aagcccaaaa agaagaaaac ccccgagccg
    18781 atgaaaaagt ccgcccccta caagccgacg aaagacttcg gcgccttgct cgatgagttt
    18841 gaaggtgctc aaaagcccaa gattccgaaa acggcgaaac aatccaagaa gaggcacgaa
    18901 ccgaaaaagc ttgtagcgag cgtggaggtg gaagaagccc ttcaggatcg aaatgagagc
    18961 agcgcgacgt ggtcctttgc cgacacctac cgcaaatgga acgagatcag cgacgaagaa
    19021 ggcgcactga aaaaggcggt gaaagagaaa gcgaagaaac cccgatcgga agaacccacc
    19081 ttgagcgctc tgcgcacgat gttgcctgag gttgtgttgg atctgcatgg gatgaccgtc
    19141 agcgaggcgg agcgcgaggc tcgggcattt ctcgccacag cgttggagca caagatccga
    19201 aaacttgcct tcatcgtcgg gaaagggctg cacaatgaag cagggtactc gctgattcga
    19261 gaggaggttg aacggattct caggctcagc aggaatgtgc gggagttctt ctccccgcct
    19321 gcccggtatg gtgggagcgg agtgatctgg gtcatcttga agggatgaaa aaaagcggcc
    19381 gggagtccaa gccgccttgt aatgtcatac gcttatcgct cgcggtaaat aattcggcct
    19441 ttcgtcaaat catacggtga gagcgctacc cggaccgaat ctccggggac aattcgaata
    19501 tagtgtttgc gcatcttgcc cgaaaggtgg gcgaggatga catggttgtt ctgcagttca
    19561 acgcggaaca tcgtattggg cagtgcttca cgcacgattc cttcaacttc aattgcttct
    19621 tctttagcca caaatcctcc taagttactt gctccaaagc gttaaaagtg taaacgtaca
    19681 acacattata gtcaagcgcc gaagatatcc acgtatccga ggatccacag gacgaagatg
    19741 atgagcggga gtacatactg gaagtagggt ttcaggattt tcgggaattc cgccccttcg
    19801 gtgcctttat ccgcctcggc gatgaagttg tcccaacccc atccgtatct ccagctcgta
    19861 taaatcgaga agatgaacgc tccgatcggc aggatgttgg agctgatgat gaaatcttcc
    19921 agatccagga ttccgctgcc cggtcccagc ggctggaacg atgataagac gttgaatccg
    19981 agaatcgccg gcagactgag cccgaccatt gcaaggccgt tgtagatcgc cgcttttcgg
    20041 cgggaaacac cgtgaacatc gatccaatag ctgacaatgt tttcaaacac tgcaatgacc
    20101 gttgaaagtg cggcaaagct catgaagagg aagaacagcg ttccccagaa cagaccgccg
    20161 cgcatctggt tgaagatgtt gggcagggtg atgaacagca aagacgggcc ggctcctgct
    20221 tcgacgttga acgcgaacgc cgccgggaag atgatgaggc cggcaaggag cgccacgaac
    20281 gtatcaaggc tgatgatgcg aatcgcctcc ccggtgagcc ggtgtttgtc gctggtgtag
    20341 gatccgaaga tcgagatcga tccgattccg aggctcaggg tgaaaaacgc ctgattcatc
    20401 gccgcataga tcgcattgcc tacgcctgcc tccgccattc gggaaaagtt cgggaccaag
    20461 taaaacttga gtccttcgcc accgcctttc agagcgatcg agtttcccgc cagaatgatc
    20521 aagaggagaa gaagaccgac catcatgtat ttggtgatct gttcgacccc cttctgaagg
    20581 ccgatgccga cggggatcat cccgaggatg attgccaaca ctccccaaaa aatcatattt
    20641 cccggtttgc cgagcatttc tccgaaaaca ccgccgacac ccgctgcgtc caagccgaca
    20701 aaggttccgc caagggtttt aaaaaggtag gagagcaccc agcccgatac catcacataa
    20761 aaactcatca gaacatagtt gccggcgatc gccacatgtc cgtaccaatg ccagcctttc
    20821 ttttcagggg tcagggtctt taaggacagg ccgatgtttt gcttgctagc ccgtccgatc
    20881 gccagctcca tgaccatcac cgggagtccc agaatcacca aaaagaggag atagatcagg
    20941 acgaacgccg ccccgccata ccggccggtg atgaacggaa aacgccacac gttccccagt
    21001 ccgattgcac accctgcgga cagcaaaata aagcctaaac ggcttcccaa tacctctcgt
    21061 ttctgtgagt tgctcatacg ctttactctc ttatggtgaa tttgtatcga tttgatacat
    21121 acgtgcaatc ttataaataa aatcgttaaa atttcaacat catcatacaa ataaaacccg
    21181 ttttgttgac atcaaatgct ttcatcggca agatacgtac aatgaatata cagtgaggtg
    21241 ggagtatgcg gcaaacaagc gctctgggaa aatacattca agcatatgga actgagaatg
    21301 aggcgatgat cggctttgtc gcgaacttgg atctcatcgc caaggtggac gaccagatcg
    21361 ccgctcgaat cgtcaatgaa ctcagcgacc agcgaaccca cctgaagctt atagccagtg
    21421 aaaacttctc atccctgtcc tctcaggctg cgatgggaaa tctgttgacg gataaataca
    21481 gcgaaggctt tgcatatcac cgcttctacg ccggctgtga caacgtcgat gcgatcgaag
    21541 aacgaggcgc cacctatgcc aaagagcttt ttggagctca gcacgcctat gtccagccgc
    21601 atgccggagc ggatgccaac ttggtggcgt attgggcgat tctcaacacg aaggtgaccg
    21661 tcccttcctt ggaagagatc ggaatctcca accccgccga tttgagccgg gaggattgga
    21721 acaggattcg tcatgcggtg ggaaatcaga agctgctcgg tcttgattac tactccggcg
    21781 gtcatttgac ccacggatat cggcagaaca tcagcgcgca gatgttcgat gcgtactcct
    21841 attgcgtcga taaagaaacc ggcctcttgg attacgatgc gattgaacgc caggcgcttg
    21901 agctgaagcc gctgatcctg cttgcaggct atagtgcgta tcctcgggcg ataaacttta
    21961 aacgcctttc agagattgcg aaaaaagccg gttcggtctt catggtggat atggcccact
    22021 tcgccggcct ggtcgccggg aaggtgttta ccggcgaata tgatccggtg gcttgggcgg
    22081 atgtggtgac caccacaacg cacaaaaccc ttcgcggtcc ccggggggga ttggtgctct
    22141 gcaccgaaga gtatgccgaa agcgtcgaca aaggctgtcc ccttgtcctc ggcggaccgc
    22201 tgccgcacgt gatggcggca aaagcggtag cgttgaagga agcgttggat ccttcgtttg
    22261 cggactatgc ccataagatc gtcgaaaacg cccgagcgtt ggctcaggcg tgcatcgatg
    22321 aagggatcac ggtagccacc ggagggacgg ataaccacct gatgctgctg gatgtgcgtt
    22381 ccttcggtct caacggccgg caagcggaaa gcgcccttcg ggaggcgggt atgaccctca
    22441 accgaaacag tcttcctttc gatccaaacg ggccgtggta caccagcggc ctgaggatcg
    22501 ggacgccggc cctcacgact ctcggaatgg gcaagcgtga aatgaaagag gtcgcttcca
    22561 tcatcgcctt ggtgttgaag aacacgaaac cggttatcct gaccaaaggc gagaacgccg
    22621 gccaaccctc aaaggctcgg gcagtgacgg agccgggcgc cagagaagaa gcaaaggagc
    22681 gagtccttgc attgttgggc aggttcgtgc tctatccaca gttggatctg cagtttttgc
    22741 agacggcttt tcccctcgac aagcaagggg tagaatggta gcattgacac cgtacgcgga
    22801 ggagcaagat gagcaggatt ttagattcat tgcgctatag caaagatcat gaatgggttc
    22861 gcattgaagg tgaaaaagcc tatgtcggca tcaccgacca cgcccaggat gagctcgggg
    22921 agatcgtgtt tgtcgagttg cccccgattg gcgagcggta taaaaaagat gaggagatct
    22981 cctcgatcga gtcggtaaaa gcagccagtc caatcatcaa cccgattgaa ggagtcgtcc
    23041 ttgaggcgaa tgaagatctt gacggctcgc cggaattgat caatgaagac tgttatgcaa
    23101 accacctgta cgttctcggg tctttttctc aagaagatta cgattccatg atggacagca
    23161 ctgaatacgc atcctacctt gcctcactgt aggcaagagg cggagtatta catgatttac
    23221 ccctacattc cccacacgga tgaggatcgg gcgcagatgc ttgaggcgct cggtctttca
    23281 tcgatcgacc ggctctttga ggtggtcggt gaggacctgc tcctcgaaga ttcggttccg
    23341 atcgcccata gccgaaccga ggatgaagtg cagcggatga ttgattcgat cgctcaacga
    23401 aacatccgag gaatctcgtt tctcggcggc gggtgttacg accatattat tccttccacc
    23461 gtcaaggcgc tttcttcact tccttccttt gtaaccgcct acaccccgta ccaggcggag
    23521 atgagtcagg gattgctcca ggcgatcttc gagttccaat cgatggtctg tgaaataacc
    23581 tcgatggata tcgcgaacgc ttcgctgtac gacggttcgc atgcgatcgt cgaaacggcg
    23641 cttttgatgg tgaacgccaa gcggggcgcc aagaaggtgc tggtcagcga gacgatccac
    23701 cccttcgccc tcaaggcgct cgggacgtgg gttcttggaa cggagtatga ggttgtaccg
    23761 attcaggaga aggatggcgt tgtcgacctt tcaaacctcg tcatcggcga tgatgtcggc
    23821 gggctcatcg tgcaatcgcc caaccgctac ggatttgtcg aagactatag cggcatagcg
    23881 gatctccttc acgagcgcaa agcgctcttt gcgatcagca gcgacccgct cagcctggcg
    23941 attcaaaaga ctcccggcca gtggggagcg gacatcgccg tcggcgatac ccaaagtctg
    24001 ggtctcagtc tttcttttgg cggcccgtcc tgcggctaca tggcggtgaa acaaccgctg
    24061 ctcagaagaa ttccgggtcg ccttgtcgga atcactaccg acgccgaagg aaaaagaggc
    24121 ttcactctga ccctccaagc acgggaacaa cacatcaagc gggagcgggc gacgagcaac
    24181 atctgttcga atcaggcgct tgccgccctg atgacgacga tccacctttc atcgctcggg
    24241 tggcatggga tggtcgaagc ggcccggcaa tcctacgcca aagcgcacta cctggctcat
    24301 catctcagtc agcttcccgg ggtggaggta atgaatgccc atccgttctg gtgcgagttt
    24361 ccggtcgtct ttcacgatgc gaagcggatg cggaagttcc tccaagagct ccgcaacgaa
    24421 gggatctttg ccggtgtccg gctttccgcg ttgacccgca aagataccga tgaactcgtt
    24481 ctccttgtag cggtaacgga aaagagaagc cgggaagagc tggagatgta tattgcggcg
    24541 gctcagaggg tgatgaaatg attgaaccgt tattgatcga taaaagcaaa aaagggcgct
    24601 tggcgtattg ttatccttct ctggatcttc cccggtcctt cgaccaggag cttgcgcatc
    24661 ttccgtccca tcttcaacgg gaggggagcg cccgcctgcc ggaagtctcc gagctcgatg
    24721 tggtcaggca cttcacccgg ctttcgagga tggttcacgg ggtcgacaac gggatgtacc
    24781 ccttgggctc ctgtacgatg aaatacaatc cgaagctcgg ggaagagatc gcccggatgg
    24841 ctcagttcac cgagatccat ccacttcagg acatctcgac ggttcaaggg tgtctatcgg
    24901 tgatgtacaa cctcctggaa gacctttcct ccattaccgg aatgtcctgg ggatcgcttc
    24961 agccgtgtgc cggcagccac ggcgaataca ccggtcttaa aatgattcgc tcctactttt
    25021 tgaaaaaggg cgacgttggg cgaaccaagg ttctgattcc cgtcagcgcc cacgggacca
    25081 accccgccag cgcggcggtc aacggcttcg atgtggttga aatcgcttca aacaggaaag
    25141 gattggtgga tctggctgat ttgaagagca agctctccga tgaggtcgcc gccctcatgt
    25201 tgaccaaccc caacaccctc gggctttttg aagaggatat ccttgcaatc agcgaagcgg
    25261 ttcatgaagc gggagccctg ctgtactatg acggggcgaa cctcaatgcg atcatgggca
    25321 aaacccgccc cggcgacatg ggcttcgacg tgctccacct caaccttcat aaaacgttct
    25381 ccacccccca tggcggcggc ggcccgggca gcggaccggt aatggtcaac gatctgctcc
    25441 gcccgttcct tccgaagccg gatatcgatc tgcgggaagg ggggtatgtg ttcgattggg
    25501 accggccgga ttcgatcggg aaggtgagcc tgttctgggg gaacttcctc gtttttctgc
    25561 gggcgtatgt ctacattctc cgcatgggga gcgaaggcct tcgagccgcc gccaatcatg
    25621 cggtgctgaa cgccaactat ctggcaaaaa aattggcgcc gtactatgag attccccata
    25681 ccggcggaat catgcacgag tttgtgatca gcgtcaaaaa ctacaaagag cagtatggag
    25741 tcaccgccga ggatttcgcc aaagcgctga tcgatgaagg gtaccacccc ccgacggtgt
    25801 acttccccct gatcgtcgac gaagcgctga tgatcgaacc caccgaaagc gaaagccttg
    25861 agagtctgga cgcgttcatc gctgcgatga tccgcatcgc ggaaaaagcg aaaagcgatc
    25921 ccgcctcgct gaagaaggcc ccggt
//
LOCUS       DAOSLI010000025        25692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2136_length_25692_cov_27.359988, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000025 DAOSLI010000000
VERSION     DAOSLI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 25692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25692
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2136_length_25692_cov_27.359988"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLF67_05130"
     CDS             <1..354
                     /locus_tag="PLF67_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOE89429.1"
                     /translation="YLEAVLALSEEHEKVRTTDVALRLGVSKPSVTRAMRNLSDGGYI
                     EQEAYGDIKLTEKGRIKASQIYFRHKTITTFLHEILGVDPEVAEADACLIEHDISNET
                     MEKLAIFMRKLEEQG"
     gene            475..1788
                     /locus_tag="PLF67_05135"
     CDS             475..1788
                     /locus_tag="PLF67_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89430.1"
                     /translation="MKKSVYFILIIALLLPAAIFAAGAKEQAVDETKPVTIVYWTHDD
                     AARTKLENELIEKFQAANPHITVERSTRSAQKQIELVQTAFAANEGPDMFNLPIENQY
                     AYITNGRVAPIDYVAAGFKSKQDMLDKYMDGILDTVTVDGEVYGLPLELTNWAIYVNK
                     KVFASAGLDAEKDYPKTWEQMADVSEKLVIRDGDILVRRGFDFRYPYYLIMFVPMVEQ
                     LGGSLLSADGKKAIINDDAWIKALTFMQEWGPLGRNLGSTTYQAPRYLFNADNNDIAM
                     AHTGLYQEGRMKTENPKFFESDDWMVVPFPVFENAVKDVASAYYGHFFMVNADSDPAV
                     QKATWKLIGFLLSHGEDYLTRGGNLIQPTKALLASDTLKNMPYSEVFINDMARANMVY
                     YGSDSAEIQTQIRNAVESVMLSGESPQKALATLRSAVQEIIDERH"
     gene            1857..2792
                     /locus_tag="PLF67_05140"
     CDS             1857..2792
                     /locus_tag="PLF67_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE89431.1"
                     /translation="MGTKQYKGIERKQARWGFAFVVPSLVFFSVFNFYPILNAFYTSF
                     YDRRALSKLKPKFVGFGNYVRLFDPARAANPLSFYNSLKATLTFTLGTFIPLLIVSLL
                     LAILISNLKSQKAKRFLQISYYTPAILSSVVAATIWMLIFDPRGLGNQWMNLILNTPG
                     VDHKWKTDPVMLQVSTMLVYFWKYIGYFVILFITGLATIPQTIYEAATIDGANKSQTF
                     WRITLPLLKPTVVLVSIMAMLQCLKTFSTQYMFYTNGSTRAPINVITFNIYITGIQDQ
                     YLGRASAMSVVLFVLMLVLTLFQFRTTKSGENIDY"
     gene            2802..3650
                     /locus_tag="PLF67_05145"
     CDS             2802..3650
                     /locus_tag="PLF67_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89432.1"
                     /translation="MQIRRTFNPINLLIYALLLVMVFFTLTPIVFMVTASMMTKNQIL
                     RMPFNWIPESFNWINFKRAIVGLDENYIFLRNIVNSLVVSVTVAITTVLLGSLTGYGL
                     AKFRFRGRNVIFIMIMATMMIPFEAIMIPLYMVVMKLRIQNTYVGLILPFLVSAFGIF
                     QMRQYLSTFPTEFLDAARVDGMSEPGIYWQIVLPNAIPVIATLSILAFRSQWDNLLWP
                     LLVSQKDKMKTIPQYITSFAAERNTDEGAMMAAALIASIPMFVLFMSLTKYFIGGSAV
                     YESRKG"
     gene            3655..4269
                     /locus_tag="PLF67_05150"
     CDS             3655..4269
                     /locus_tag="PLF67_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOE89433.1"
                     /translation="MDRNAIAGKDTKVFLFDMGNVVVKGIGISEKIAKRLGVPRDQFE
                     LDYHHWIYPLMEGEISAEQYWRHAEKVFGKRIEEDLLAVCFTPYLNIEVVGTITKLRE
                     KGYKVVCASNTYASHWEILRKMDALSLFDALYPSHEVGLSKPAHAYFRHILEAEGVKA
                     SEAYFVDDSIEHIESARTMGIPSLHYSGKAASRELKRVFEPLLG"
     gene            complement(4258..5028)
                     /locus_tag="PLF67_05155"
     CDS             complement(4258..5028)
                     /locus_tag="PLF67_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE89434.1"
                     /translation="MAYTVVFVEDEELVRREIVSSIRWEALGLKLVGVAADGAEGERL
                     IKELEPDIVITDIRLPAQDGLSMLASCPVDHAIILTGHTDFTYMKQAIRLGVFDYLLK
                     PVSDEELEEALVSLVQKIQEEDRDVEQINRRHREHDHSITLPKHFKNHVIDSAVAFIV
                     DNYSQSVGLQEAANHLGVSESHLSRLFKEVTGLNFLQYLNAYRINKSVEAMSDPKKNI
                     AEIATSCGFPTPGYYAKIFKRFFGRTPTQFRDILGSPQ"
     gene            complement(5032..6777)
                     /locus_tag="PLF67_05160"
     CDS             complement(5032..6777)
                     /locus_tag="PLF67_05160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOE89435.1"
                     /translation="MADRSRTLFVRLLIYFLIVLLLPLLVFSGYYLFLGDRSQSKYLS
                     EQSLGVIRRDALIVGNVLEEYRHKAYLLSTDELIVEVMKHDSPEFLNRVIHEVYPLLF
                     SVMKGDTYLASANLVSNTGRIRLSTHAFPKMFDLRYHTSTSELNSVIAQNLNLSQTAS
                     LITTRGHRTAENGRQVVASILRRIYDGEGTNLGYLVIDLFGEALSTGINSERLFSDLY
                     LIDQKEFHATSLIHTDMHGSFDKFPPLLSLKGDYTPRSVQVGSTVVAVAPVANTTNLY
                     LAGTLSTVFFSRNISRWYLVFTGTLIAGFALAMMFSFLFSRSIAHPIASLARRMDRVD
                     RMNLETRPVKSTITEFAQLENSYNMMIDQIRTLLEQTREEQKKLSEAERKALESQMNP
                     HFLFNTLNTIKALARINNQEEIYTITVKLGKLLRSTIDSHESEATLAHSIALIDSYLT
                     IQKLRYGEKLVTSIHLDPACAHIKTPKLIIQPLVENAIIHGLEPKAGNWFLAVRVEAV
                     GRHIQITVEDNGVGFDRETLPQDLNDLAHSTHVGLYNVYRRLSLAYQNELEFSIDSTV
                     GKGTIVRISLPIGRR"
     gene            7136..7675
                     /gene="ilvN"
                     /locus_tag="PLF67_05165"
     CDS             7136..7675
                     /gene="ilvN"
                     /locus_tag="PLF67_05165"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOE89436.1"
                     /translation="MSKREKRYTLAILVNNNPGVLMRVVSLFSRRGYNIDSLSVGETE
                     DERFSRITIVVPGDRAVVEQIKKQVAKLYDVIQVYEMTRTASLQRELIMIKVNASVAS
                     RTEVVELAQIFKAKIIDVTNTTVSLEMTGTLDKIDSFLSLISPYGIVELARTGITALE
                     RGSRALSSIGFDEIQTGEE"
     gene            7675..8667
                     /gene="ilvC"
                     /locus_tag="PLF67_05170"
     CDS             7675..8667
                     /gene="ilvC"
                     /locus_tag="PLF67_05170"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOE89437.1"
                     /translation="MSTMYYDQDADLTLLEGKKVAIIGYGSQGHAHALNLHESGVDVV
                     VGLYEGSKSWNVAKEAGLEVMTASEAAKAASVIMLLLPDEKQAKIYRESIAPYLTKGD
                     YLAFAHGFNIHFGQIAPPEDVNVIMIAPKGPGHTVRSQFQEGKGVPSLIAVHQDPSGN
                     SKEIALAYAKGLGAGRAGIFETSFREETETDLFGEQAVLCGGITALMKAGFDTLVEAG
                     YQPEMAYFECVHEMKLIVDLINQGGLSYMRYSISDTAEYGDYTTGNKIITDETKKVMK
                     EVLKDIQEGTFARTWLLENQVGRPYFNARRRMERESLLEKTGAKLRSLMSWLKR"
     gene            8679..10283
                     /locus_tag="PLF67_05175"
     CDS             8679..10283
                     /locus_tag="PLF67_05175"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOE89438.1"
                     /translation="MRKERVYIFDTTLRDGEQAPGYSMNLDEKIRMALQLEALGVDVI
                     EAGFAIASEGDFESIKAVCEHVKKPVIASLSRSLEKDIDASFEAIKGAERGRIHLFLA
                     TSDLHLKYKLRMSREEVLERSTKMVAYARNKIDDVQFSLEDATRTDLDYLCTVVEAVI
                     KAGANVVNLPDTVGYASTGDLQRMVETVMNRVPNVDKAILAVHCHNDLGMGVANTLTG
                     LNAGARQAEVTICGIGERAGNAALEEVVMNLKTRGDIYPFETNINTEQITQSARVLKQ
                     ITGVKIHPSKAIVGSNAFAHESGIHQHGMLANSLTYEIMTPESVGVKKTSLVLGKHSG
                     QHAFEQRLGELGYSFTKEEFSRLFNEFKNLADRKKNVEERDLIALVESAHQGSPTIWE
                     LDSFVVNSGNLITSTACVTLKKGDEKHQEVAWGTGPVYAALRAVEKIVDHPFSLEDYT
                     LQAVTEHRDALGEVFVKLRDERGTYRGRGVSTDVIEASILSCLAAINKMVDGAPAKRE
                     HPFKPTNDHSFENDMLMNNSDKRRFM"
     gene            10274..11884
                     /gene="cimA"
                     /locus_tag="PLF67_05180"
     CDS             10274..11884
                     /gene="cimA"
                     /locus_tag="PLF67_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOE89439.1"
                     /translation="MHVKVAIFDSTLRDGSQGEGISFSVEDKLKVVRALDALGIDFIE
                     AGNPGSNPKDQEFFDRARTLELKHAAIVAFGSTRRKGIEAKDDPNLQSLIGAKTKIVS
                     IFGKSWDFHVTEILRTTLKENLAMIEDSIRFCCDAGVEVFFDAEHYYDGYLADKEYAL
                     ETLRAALKGGASTLVLCETRGGIIPSEFERITRETMAAIPNAQWAVHAHDDIGTAVAS
                     SILAVQCGVGQVQGTLLGIGERCGNANLSTIIGILQTKLGYECLNGESLEFLYPTCRA
                     IGEVANVRIARSMPFVGKSAFAHKGGMHIDGVAKNPASFEHIDPTVVGNERRLLMSEV
                     AGRALMLRRIAKVVPDLDKDDPVTVTLMARLKELEAVGYQFEGAESSFDLVIRRHLNL
                     YKPYFSLIHYQTIGTAPYLDPDSTATHAVVIKVWVKGRSAIAAAEGEGPVNALDTALR
                     KVLEEFYPTLKSVHLTDYKVRVLDSKEATASTVRVLIESTDGTESWSTIGVSKDIIQA
                     SWSALSDSIEYKLIKDGIVPNGECEKEI"
     gene            11893..13155
                     /gene="leuC"
                     /locus_tag="PLF67_05185"
     CDS             11893..13155
                     /gene="leuC"
                     /locus_tag="PLF67_05185"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOE89440.1"
                     /translation="MTMTQKILAKAAGKASVRAGELIMADLDLVLGNDITAPVAINEF
                     PKFGKETVFDREKIALVPDHFTPNKDIKAAEQVKAMRTFAHDHGIVNYFEVGQMGIEH
                     ALLPEKGLVGAGEVVIGADSHTCTYGALGSFATGVGSTDMASGMATGKAWFKVPSALK
                     FNLTGTLNRHVSGKDVILHIIGLIGVDGALYQSMEYSGEGVKNLSIDDRFTIANMAIE
                     AGAKNGIFPVDEVTLGYLAEHCPRTPRIFEADEDAEYEAVYEIDLSAIKSTVAFPHLP
                     SNTRPIDEVGEVKIDQVVIGSCTNGRISDLRQAAAVLKGKKVAPWVRALIFPATQKIW
                     KQAMNEGLFEIFVDAGCAVSTPTCGPCLGGHMGILAEGEKAVSTTNRNFVGRMGHPKS
                     EVYLASPAVAAASAVTGYITDPNTIEEA"
     gene            13157..13651
                     /gene="leuD"
                     /locus_tag="PLF67_05190"
     CDS             13157..13651
                     /gene="leuD"
                     /locus_tag="PLF67_05190"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOE89441.1"
                     /translation="MRVSGTVFRYGDNVDTDVIIPARYLNTSNHAELALHCMEDLDAT
                     FKDKVKKGDIIVADANFGCGSSREHAPIAIKESGVSCVIARSFARIFYRNSINIGLPI
                     LECPEACADIKAGDTVVVDFSTGVITNKRSGNTFQSEPFPPFMQELIQEGGLANYVAK
                     GGIA"
     gene            13651..14703
                     /gene="leuB"
                     /locus_tag="PLF67_05195"
     CDS             13651..14703
                     /gene="leuB"
                     /locus_tag="PLF67_05195"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOE89442.1"
                     /translation="MEKKRIAVIAGDGIGVEITREALNVLKKIAPDGFVFEHHKAGGE
                     ALDAFGVPLPEETLNGCKQSDAVLLGSLGGPKWDTVPSHLRPEKGLLGLRGGLGLFCN
                     LRPAILYPQLAGACPLKNELVEGGLDIMVVRELTGGIYFGERGREGDSAFDTERYSKA
                     EVERIGRKAFEIAMNRSKRLCSIDKANILISSQLWRETINALSKEYPEVSVTHMYVDN
                     AAMQLVRNPRQFDTIVTNNIFGDILSDEAAQITGSIGMLPSASLGEGGVGMYEPIHGS
                     APDIAGKDIANPIAMILSAALMLRHSFGMEREAKRIEDAVSGVLDQGYRTADIMSGGM
                     TLVGTKEMGRRITEAL"
     gene            14716..16389
                     /gene="ilvD"
                     /locus_tag="PLF67_05200"
     CDS             14716..16389
                     /gene="ilvD"
                     /locus_tag="PLF67_05200"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOE89443.1"
                     /translation="MKRRSAQMTEGPQRSPHRSLMKALGWTDSEIHMPIIGVVSGANE
                     IIPGHIHLSRIVEAVKSGVRHGGGNPVAFPVIGVCDGIAMGHTGMKYSLVSRELIADS
                     IEVMANAHPFDALVFVPNCDKVVPGMLMAAARINIPSIFVSGGPMLAGKKDGREPINL
                     SRMFEAVGSYALGAIDDAELLDWENNSCPTCGSCSGMYTANSMNCLTEAIGMGLPGNG
                     TIPAVYAERDRLAKEAGYRIMELLKEDVRPLDIMTEKAFENALAVDMAIGCSTNTMLH
                     LPAIASEAGIDLDIFIANEISAKVPNLCHLAPSGNDHVEDLFYAGGVLAVMHELGPLG
                     VLHGDLPTVSGKTIGRLWAEHPVRNHEVIRPLNNPFDKTGGIAVLKGNLAVDGAVVKR
                     SAVDPSMLVHSGPARVFDSEEEANEAIFSGRINKGDVIVIRYEGPKGGPGMREMLSPT
                     SAIAGMGLDKDVALITDGRFSGATRGSSIGHVSPEAASGGLIALVEEGDEILIDIPGG
                     ELTLKVDEATLNKRRASFKEKEVVRYGGYLDRYRKQVTSANTGAIFAQE"
     gene            16393..18072
                     /gene="ilvB"
                     /locus_tag="PLF67_05205"
     CDS             16393..18072
                     /gene="ilvB"
                     /locus_tag="PLF67_05205"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOE89444.1"
                     /translation="MKLRGAEIILECLLEQGVDTVFGFPGGAVLPLYDALYSYRDRIN
                     HILTSHEQGAAHAADGYSRSTGKVGVVFATSGPGATNLVTGIATAYMDSVPVVAITGN
                     VTVPLLGKDSFQEVDITGITMPVTKHNFIVKDVNDLARTIRLAFQIAQEGRPGPVLID
                     VPKDVTVEYAKYERQVIEPVKPRSDRISEQALSAALELIHSAERPVCFVGGGVVRARA
                     SKELTEFIEKIDAPVCASMMGIGAIPTTSTRYTGMVGMHGTKVSNMIVSMCDLLIVIG
                     SRFSDRVVSKASAFARGANIIHIDVDPAEIDKNITATVGIVGDVRIVLKELNRRIDKQ
                     IPSNGWMDQVYSYKERFPIRIESENQRSKEVLKALQKVLPEGAFVVTEVGQHQIWAAQ
                     FLKHTQCGHFLTSGGLGTMGYGTGSAIGAQVGNPEARVVSVAGDGSFKMNCNELATIA
                     RYRLPVVILIMNNRTLGMVRQWQTLFFNSHYSETTLDTPIDWVMLANAYGLKGMRIAK
                     EQDPLPILKEAFALNEAVVIDCEIPTDDKAYPMVAPGASIEEMLGVKESSK"
     gene            complement(18006..18269)
                     /locus_tag="PLF67_05210"
     CDS             complement(18006..18269)
                     /locus_tag="PLF67_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE89445.1"
                     /translation="MGTMTLGQVIKHLRYEKNMTQEQLIEKAKLTRSQLYYIEADRRG
                     IHLTTLFAICNGLGVSLVEFVSYLDDSFTPSISSIEAPGATIG"
     gene            18471..18920
                     /locus_tag="PLF67_05215"
     CDS             18471..18920
                     /locus_tag="PLF67_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOE89446.1"
                     /translation="MGAFNHGELTVICTNLRKACEKQLRSEEAALFGNLATYFSGKTE
                     CPGQATLQEIGAVIDEELATLYPQANEQAEAAGDRGAKRALLWGEKVTRLHKSFLMRL
                     ERNPKLLDETPVWVCESCGFLFVGAVSPAICPICKVPNIKIHKMEVR"
     gene            18920..19390
                     /locus_tag="PLF67_05220"
     CDS             18920..19390
                     /locus_tag="PLF67_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOE89447.1"
                     /translation="MVASRNLRLCTKDCICLFVCPTGATDTESGQIDASKCLDGCRLC
                     VDACPGGAIYLVPRNYPKPQPKREDVVKALFALAESKVKTRALANGLGDDYPLARKAL
                     DFSLEVLAEDCVREGGYMLPQSAVTKEFLKNLKERYGDDPAFPVKAVERLLDLL"
     gene            complement(19385..20191)
                     /locus_tag="PLF67_05225"
     CDS             complement(19385..20191)
                     /locus_tag="PLF67_05225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOE89448.1"
                     /translation="MPSIKLICTDIDGTLLRPDHTISPRTKRAIQEAHKRGIIVALVS
                     GRIGKSLVKIQEELGITGPLGTLNGSLVLDEKGRSILEHPLQKDEIHQILEWAKQTDL
                     NAFVYTNTNWYASTEGYWTEYELKISGFKGIVRSFYEIEGNLGTDEKPFKVLCMDKDP
                     EYLSRMERQLIQQMGSRFTILRSGPIYLEIFHRGVDKGDAVKALGSHYALPREAIMAV
                     GDYYNDVAMLKAAGYSVAMGNAPDDIREMADVVTRPNTEDGLALAIEAVL"
     gene            complement(20244..21353)
                     /locus_tag="PLF67_05230"
     CDS             complement(20244..21353)
                     /locus_tag="PLF67_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOE89449.1"
                     /translation="MSPTDRKISFIAASTLLLSSLEHLIPKPLPFLRLGLANLPLLLI
                     LQSIDYPSYFFILLLKAIGQGMVSGTLFSYLFLISLAGTMSSGYAMKLLKSLLKERVS
                     LIGISVVGAFVSNLSQLQVASWIVYGPAIWVAAPLMLGLGMITSIILGVFGERYLRHG
                     SFPAAFLADSFEPVLPTAEERPHLVERTVALIIAIGAILFAKGPIPLLGIAVLMYVIQ
                     GISGRKIRLFPPLMLLSSLIVLSLFEPNGQVIFTVGTLALTKGSIALALTKGLRLIAL
                     LSASQSLVSSNPRLKGRLGSLLVLALSYFGILTSCFRTAKGSLLERVDGALFCAAEGS
                     LQVSANNQQERSSSVLIIITAILVIGISLASRVWG"
     gene            complement(21340..21711)
                     /locus_tag="PLF67_05235"
     CDS             complement(21340..21711)
                     /locus_tag="PLF67_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOE89450.1"
                     /translation="MKIPSSLVGDLVIILLCAAAVGFFVFALGQNTGNGYVSVQTADG
                     VYRYSLKNDRSVTVTGPLGDTLIEIREGKARIVDSACPNKTCTFQSPIDRSGSFIACL
                     PNHVLLTVVGPMPQEIDDVAY"
     gene            21871..22578
                     /locus_tag="PLF67_05240"
     CDS             21871..22578
                     /locus_tag="PLF67_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE89451.1"
                     /translation="MKLIYVADGCNDDREAMKQYLELSGFEVQAFEELHGLQVALNKQ
                     APDLLLLEVHHSDGDGFTFLKKMKQSHTFPVIFVTNRSAESDRIFGFELGADDYVCKP
                     FSAKELTLRVKALFTRIELSSSRKDHATYWTLGSSRLQLDEESHIFSLDGEVISLTTM
                     EWRIMSYLVANAGILITRSQILEHCFDYSFESYDRSVDTHIKNIRKKIGPMGTEWIET
                     VRGYGYRFGGQSIPHHG"
     gene            22585..23277
                     /locus_tag="PLF67_05245"
     CDS             22585..23277
                     /locus_tag="PLF67_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE89452.1"
                     /translation="MKPLIYIVEDHAVIRDGVRQYLELSGYDVREFGDLTSTKKALQT
                     ETPDLLIQDVMLPDGDGFLFIKELKKEKDIPVIFMTARIEESDRILGFELGADDYVTK
                     PFSPKELMMRVQAVLRRTSGPQSDTFYIGDEVLTFDQGTHQAHINGEPMSLTAAEWRV
                     LEYLIENLNQLCTREDILKYCFDYTYESYERVVDTHIKNIRAKLGGGDWIETIRGYGY
                     RFLGSPKKEGAG"
     assembly_gap    23339..23438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23439..24520
                     /locus_tag="PLF67_05250"
     CDS             <23439..24520
                     /locus_tag="PLF67_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE89453.1"
                     /translation="KPANRTTRRVRTLSYTMELNAQTPSLREERTIRSQEIPLSSEIG
                     PSDIAGTFTLTVNGEEVGYIDVLTYTPFTYQSTSFILKALVAPFVWSIPLGLILALIL
                     GAVISRRTQRYSDGISSALEELSRGEHNVELPEAKFDEQETINASIRALDKTLLHNKQ
                     SRQAWLRSISHDFNTPLTAMGLLLEGIRDKVFPLSPETIAKVQGEHALLASRIAQVSL
                     YAHLLGQEPPIDERPFNAHEFAKEILQSTRSERIELTVNTEELIGDRTLLGYAANALL
                     SNALNASGEPVRWTIDSRSMEFTNKGTLPQEIDFFEPWERGDKARTSSGSGMGLPIVY
                     QIMRLHQGNATIEQQNDDVKCVISW"
     gene            24978..>25692
                     /locus_tag="PLF67_05255"
     CDS             24978..>25692
                     /locus_tag="PLF67_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89454.1"
                     /translation="MKKKFLTLALVLMVAVTLFAATPIEVSGSLTAGYKFTFGGATPG
                     TVGNNKGKATIVSSFTGDHWKVSIGGNLAYGANKGLASATAEIYLDKALKDQGVDMGD
                     FKVTLHAGTGVEKGAYSVFANKGALGKEAAAMVTKDNAGITLGYGSLAKLYFAFFPNK
                     AFPMVVSAQVTPVAGVDVTFGYSNESANNDLLVTAKADIAKLADLDFALASTAQFAYD
                     VDAKAMVVGVDASTTIEGIG"
BASE COUNT         5885 a         6887 c         7472 g         5348 t
ORIGIN      
        1 tacttggaag ccgttttggc gctgagtgag gaacatgaaa aggtgcggac caccgatgtg
       61 gcgcttcgcc tcggggtctc gaagcccagc gtgacgcggg cgatgagaaa cctgagcgac
      121 ggcggctaca tcgagcagga agcatacggt gacatcaaat taacggagaa gggaagaatc
      181 aaggcctccc aaatctactt ccgccataag accatcacta cgttcctgca cgaaatcctc
      241 ggagtggatc ccgaggtcgc cgaagcggat gcatgcctca tcgaacacga catttccaat
      301 gaaacgatgg agaagctggc gatcttcatg cgcaagctgg aagaacaagg ttgatgttta
      361 taaaagggtt ttgtcatcgg gagcaaaacc ctttccgtta gcgcaaaagt tttctcttgt
      421 gctttgaaag agactcctta aacttaacta tagaagactt taaggaggtc tgtaatgaaa
      481 aagagcgtat attttattct gatcatcgcc ctgcttcttc ccgccgcgat ctttgctgcc
      541 ggtgcgaaag agcaggctgt cgatgaaacc aagccggtta ccatcgtcta ctggacccat
      601 gacgatgctg cccggaccaa gctggaaaat gagctgattg aaaagttcca ggctgccaac
      661 ccccacatca ctgtggaacg gtcgacccga tccgcccaga agcagattga gctggtccag
      721 accgcttttg ccgccaatga agggccggat atgttcaacc ttcccatcga gaaccagtac
      781 gcctacatca ccaacggccg ggttgcgccg atcgactatg tagctgccgg tttcaagagc
      841 aagcaggaca tgcttgacaa gtacatggat ggaattctgg acaccgttac ggttgatgga
      901 gaagtctacg gcctgccgct tgagcttacc aactgggcga tctacgtcaa caagaaagtg
      961 tttgcttccg ccggtcttga tgctgaaaaa gactacccga aaacctggga gcagatggcc
     1021 gatgtctccg aaaaactggt gatcagggat ggcgacatcc tcgttcgccg cggctttgac
     1081 ttccgctatc cgtattatct gatcatgttc gttccgatgg tcgaacagct cggcggctcc
     1141 ctgctcagtg ctgacggcaa gaaagcgatc atcaacgatg atgcctggat caaggcgttg
     1201 accttcatgc aggagtgggg accgctgggc cgcaacctcg gttcgacgac ctatcaagcc
     1261 ccccggtacc tcttcaacgc ggacaacaac gatatcgcca tggcccacac cggtctgtat
     1321 caggaaggcc ggatgaagac ggagaacccc aagttcttcg agtcggacga ttggatggtt
     1381 gttcccttcc cggtctttga aaatgcggtg aaggacgttg catccgccta ctacggccac
     1441 ttcttcatgg tcaacgccga cagcgacccg gcagtccaga aggcgacctg gaagctcatc
     1501 ggattccttc tttcccacgg tgaagactac ctgacccgcg gtggaaacct gattcagccg
     1561 accaaggcgc tgcttgccag cgacaccctg aagaacatgc cctacagcga agtcttcatc
     1621 aacgacatgg ctcgggcgaa catggtgtac tacggatccg attcggcgga aatccagact
     1681 cagatccgga acgcggttga aagcgtcatg ctcagtggcg aaagcccgca gaaggcgctt
     1741 gccacgctcc gctccgccgt tcaggaaatc attgacgagc gacattgatt caatcgatct
     1801 cagccccctt ttccgggggc tgaatcttgt ctttttctct gtaaggaacc aatttgatgg
     1861 gtacgaaaca atacaaggga attgaaagga aacaggcgcg ttgggggttt gcctttgtcg
     1921 tgccctccct tgtcttcttt tccgttttca acttttatcc gattctcaac gcattctata
     1981 cgagcttcta cgatcgccgg gcactgagca agctcaaacc caaatttgtc ggcttcggca
     2041 actacgtccg cctctttgat cccgctcggg cggccaaccc gctttctttt tacaacagtc
     2101 tgaaggcgac gcttaccttc acgttgggaa cgttcattcc cctgttgatc gtcagcctct
     2161 tgttggcgat cctgatcagc aatctgaaat cccaaaaagc gaagcgcttt ttacaaatct
     2221 catactatac tccggcaatc ctctcttcgg tggtcgccgc cacgatctgg atgctgattt
     2281 tcgatcccag gggcttgggc aaccagtgga tgaacctgat tctcaacact cccggagtcg
     2341 atcataagtg gaagaccgac ccggtgatgc tccaagtctc gacgatgctt gtctactttt
     2401 ggaagtatat cggctatttc gtcatcctct tcatcaccgg tcttgcgacg attccccaga
     2461 cgatctatga agcggcgacg atcgacggcg cgaacaagag ccagacgttc tggcggatca
     2521 ccctcccgct tttgaagccg accgtggtgc ttgtttccat catggcgatg ctgcagtgtc
     2581 tgaagacgtt cagcacccag tacatgttct ataccaacgg ttcgacccgg gctccgatca
     2641 acgtcatcac cttcaacatc tacatcaccg gaatccagga tcagtacctg ggaagggcaa
     2701 gcgcgatgag cgtcgtcctc ttcgtgctga tgctcgtctt gacgctcttc caattccgaa
     2761 ccaccaagag cggtgagaat atcgattact aggagggcac gatgcagata cggcgaacat
     2821 tcaaccccat caacctgctc atctacgcac tcctcttggt gatggtcttc tttaccctga
     2881 ccccgatcgt cttcatggtc accgcctcga tgatgacaaa gaaccagatt cttcgaatgc
     2941 cgttcaactg gattcccgaa tcgttcaact ggatcaactt caagcgggcg atcgtcggtc
     3001 tggatgaaaa ctacatcttc ctccggaaca tcgtaaactc cctggtcgtc tccgtcaccg
     3061 tcgcgatcac caccgtcctg cttggcagcc ttaccggcta tggactggcc aaattccggt
     3121 tccggggccg aaacgtcatc ttcatcatga tcatggcgac gatgatgatt cccttcgaag
     3181 cgatcatgat tccgctctac atggtggtga tgaaactcag aatccaaaac acctatgtgg
     3241 gtttgattct tcccttcctc gtcagcgcct tcggaatctt ccagatgcgc cagtacctct
     3301 ccacctttcc gacggagttc cttgacgccg cccgggtgga cgggatgagt gaaccgggaa
     3361 tctactggca gatcgttctt ccgaacgcca taccggtgat cgccacgctc tcaatcctgg
     3421 cgttccgctc gcagtgggac aacctcctgt ggcctctttt ggtcagccaa aaagataaaa
     3481 tgaaaacgat tcctcagtat attacgtcat tcgccgctga acgaaacacc gacgaaggag
     3541 cgatgatggc cgccgccctg atcgcctcga ttccgatgtt cgtcctcttc atgagcctca
     3601 cgaagtactt catcggcgga tcggcggtct acgaatcacg caagggatag tccgatggac
     3661 aggaatgcaa tcgccggcaa agacacaaag gtgtttcttt tcgatatggg caatgtcgtc
     3721 gtcaaaggaa tcgggatcag tgaaaagatc gccaaacgct taggagttcc ccgcgaccaa
     3781 ttcgagttgg attaccacca ctggatctat ccgctgatgg aaggggagat cagcgccgag
     3841 cagtattggc ggcacgctga aaaggtgttc gggaaacgga tcgaagagga cctcttggct
     3901 gtctgcttca ctccatatct gaacatcgag gtggtcggca cgattactaa actgagggag
     3961 aaaggatata aggtggtgtg cgcctccaat acctacgcgt ctcattggga gatccttcga
     4021 aagatggatg cgctctccct ctttgacgcg ctctatccct cccacgaagt agggctttcc
     4081 aaaccggctc acgcgtactt ccggcatatt ctcgaagctg aaggagtcaa ggcgagtgaa
     4141 gcatatttcg tcgacgattc gatcgagcac atcgaaagcg cccggactat ggggattcct
     4201 tctctgcatt acagcggcaa agccgcttca agagagctga aacgagtgtt tgaacctcta
     4261 ttggggtgat ccaaggatat cgcggaactg ggtcggagtc ctgccgaaaa agcgtttgaa
     4321 gatcttggcg tagtacccgg gggtgggaaa gccgcaactg gttgcaattt cggcgatgtt
     4381 ctttttggga tcgctcatcg cttcgacgct cttgttgatc cgatacgcgt tgagatactg
     4441 gaggaagttg agccccgtaa cctctttgaa gagtcgggag aggtggctct cactgactcc
     4501 cagatgattt gccgcttcct gcaacccgac cgattggctg tagttgtcga cgatgaaggc
     4561 gaccgccgaa tcgatcacat ggttcttgaa gtgcttggga agggtgatgg aatgatcgtg
     4621 ctcccggtgg cgccggttga tctgctccac atcccggtct tcctcctgaa tcttctgtac
     4681 cagggaaaca agcgcctctt caagctcctc atcgcttacc ggtttcaaaa gatagtcaaa
     4741 cacaccgagg cggatcgcct gtttcatgta ggtgaagtcg gtgtgaccgg ttaagatgat
     4801 cgcatggtcc accgggcacg aggcgagcat cgaaagtccg tcctgagcgg ggaggcggat
     4861 gtcggtaatc acgatatccg gctccaattc cttgatgagt cgttctcctt ccgccccgtc
     4921 ggcggcgact cccaccaatt tcaaacccaa ggcctcccac cggatcgacg agacgatctc
     4981 ccggcgaaca agctcttcgt cctctacgaa cacgacggtg tatgccacga cttatctcct
     5041 tcctatgggc aggctgatgc gaacgatcgt tcctttccct accgttgaat cgatcgaaaa
     5101 ctcaagctcg ttttggtatg cgagtgaaag tcttcgatat acattgtata gcccaacgtg
     5161 ggttgaatgt gcaagatcgt tgagatcctg cggcaacgtc tcccggtcga acccgactcc
     5221 gttgtcctcg accgtgatct gaatgtgccg tccgacggct tccacccgga ccgccagaaa
     5281 ccaattgcct gctttaggct ccagaccgtg gatgatcgca ttctcgacca gcggctggat
     5341 gatgagcttt ggagttttga tgtgggcgca cgccgggtcg aggtggatcg aggtgacgag
     5401 cttttccccg taacggagct tttgaatcgt cagataggaa tcgatcaggg cgatcgaatg
     5461 ggcgagggtc gcttcgcttt catggctgtc gatcgtcgag cggagcagtt tccccagttt
     5521 cacggtgatc gtgtagatct cttcctggtt gttgatccgg gccagagcct tgatcgtatt
     5581 gagggtgttg aagaggaagt ggggattcat ctggctttcc aacgctttgc gctccgcttc
     5641 gctgagcttc ttctgctcct cccgggtctg ttccagaagc gttctgatct gatcgatcat
     5701 catgttgtag gagttttcaa gctgagcgaa ttcggtgatc gtcgatttga ccggtctggt
     5761 ttccaggttc attcggtcga cccgatccat ccgtcgggcc agggaagcga tcggatgggc
     5821 gattgaacgg gagaagagga agctgaacat catcgccaag gcgaagccgg cgatgagcgt
     5881 cccggtgaag accagatacc accttgagat gttcctgctg aaaaacaccg tgctgagcgt
     5941 tccggcaaga tagaggttgg tggtattggc caccggagcc acggcgacca ccgtcgaacc
     6001 gacctggacg gatcgggggg tgtaatctcc tttcagggaa agcaacgggg ggaacttgtc
     6061 gaaagaaccg tgcatatcgg tatggatcag gctggtggcg tggaactcct tctgatcgat
     6121 cagatacaga tcgctgaaca gccgttcgct gttgattccg gtgctgagcg cctcgccgaa
     6181 gaggtcgatc accagatacc cgagattggt tccctccccg tcatagattc ttctcaggat
     6241 tgaagcaacc acctgccggc cgttttcagc ggtccgatgc ccccgggtag tgatcaagct
     6301 ggcggtttgc gagaggttga ggttttgggc gatgacgctg ttcaattcac tggtggaggt
     6361 gtggtaccgc aaatcgaaca tcttgggaaa ggcgtgggtc gaaagccgga ttcgccccgt
     6421 attggaaacg agattcgcgc tggcgaggta ggtgtctccc ttcatcacgc tgaagaggag
     6481 gggatagacc tcgtggatga cccggttgag gaactccggc gagtcgtgtt tcattacctc
     6541 gacgatcaac tcatcggtgg agagaagata tgctttatgg cggtattcct ccagaacatt
     6601 gccgacgatg agggcgtcgc ggcggatcac gccgaggctt tgctcggaaa ggtacttgct
     6661 ttgacttctg tccccaagga agagatagta tccgctgaaa accaataacg gaagcaacaa
     6721 tacgatgaga aagtagatta ataagcgtac aaagagggta cgactccgat ccgccataga
     6781 caaagtgtac cgacaaactt ctcctcgaac aataggaagg aatgttcgta tattgcgtgt
     6841 ataaagatgc tatttttgaa taatgggttg acagagaagg cggaagagag gtacaaacac
     6901 tcaatacctg caggtgcagg tgcaaggagt ggagtgtgaa cggcaccttg aagatccagg
     6961 ttacccgaac gaacgagctt gccatgacga gctcgaacct tttcatctcc gccctcatta
     7021 ttctagtact tattattctc tgaccacggc tgcagaatat ctgcatcgtc cgtggaagcc
     7081 cccctgccaa gggaggtctt ttttgtatcc aacgtgttga ggaggaggga acacgatgag
     7141 taaacgagaa aaacgctaca ccctggcgat cttggtgaac aacaatcccg gagtgctgat
     7201 gcgggtcgtc tcgctcttca gtcgaagggg atacaacatc gacagcctct ccgtcggcga
     7261 gacggaggat gaacgcttca gccggatcac gatcgtcgtc cccggtgatc gagcggtcgt
     7321 cgagcagatc aagaagcagg tcgccaagct gtatgatgtg attcaagtct atgagatgac
     7381 ccgcaccgcc agtctgcagc gcgagctgat catgatcaaa gtcaatgctt cggtggcgag
     7441 ccggaccgaa gtcgtagaac tggcccagat cttcaaagcg aagatcatcg atgtgacgaa
     7501 taccaccgtc agcttggaga tgaccggcac gctcgacaag atcgattcct ttttgagcct
     7561 gatcagcccc tacggaatcg tcgagctggc ccgaaccgga ataacagcct tggagcgcgg
     7621 cagccgagct ctgagttcga tcggatttga tgaaatacaa acgggagaag agtgatgagt
     7681 acgatgtatt acgaccagga tgcggatctt acgttgctgg aaggcaaaaa agtagcaatt
     7741 atcggatatg gaagccaagg acacgcccac gcgttgaatc tgcatgaaag cggcgtcgac
     7801 gtggtggtcg gcctgtacga gggctcgaag agctggaacg tcgccaaaga agccggactg
     7861 gaggtcatga cggcgagcga agcagcgaag gccgcttccg tcattatgct gcttctgccg
     7921 gatgagaaac aggcgaagat ctaccgcgag tcgatcgctc catatctgac aaaaggcgac
     7981 tatctcgcct ttgcccacgg cttcaacatc catttcggtc agatcgctcc cccggaggat
     8041 gtgaacgtca tcatgatcgc cccgaaggga cccggacaca ccgtccgcag ccagttccag
     8101 gagggcaagg gtgttccttc cctgatcgcc gtccaccagg atccgagcgg gaactccaag
     8161 gagatcgccc tcgcgtacgc caaaggcctc ggagcgggac gggcgggaat ctttgaaacc
     8221 tcgttcaggg aggagacgga aacggacctc ttcggcgagc aggcggtgct ttgcggcgga
     8281 atcacagcgc tgatgaaagc gggctttgat acgctggtcg aagcggggta tcagcccgag
     8341 atggcgtact ttgagtgcgt ccacgagatg aagctgatcg tcgacctgat caaccagggc
     8401 ggtctttcct atatgcgcta ctcaatcagc gacaccgccg aatatggcga ctacaccacc
     8461 ggaaacaaga tcatcaccga tgaaacgaag aaggtgatga aagaggtgct gaaggatatc
     8521 caggaaggaa cgttcgcccg gacttggctc cttgagaacc aggtcggccg gccgtacttc
     8581 aacgcccgaa ggagaatgga gcgggaaagc cttctggaaa agaccggggc gaagctccgg
     8641 tccctgatga gctggctcaa acgctaaagg aggccgggat gcggaaggag cgagtctata
     8701 tctttgacac cacgttgcgg gacggcgaac aggcccccgg ctattcgatg aacctcgatg
     8761 aaaagattcg aatggccctt cagcttgaag cccttggtgt cgatgtgatc gaagcgggtt
     8821 tcgcgatcgc cagcgaaggg gatttcgaat cgatcaaagc ggtgtgcgaa cacgtgaaga
     8881 aaccggtcat cgcatcgctg agccgttctc tcgagaagga tatcgatgcc tccttcgagg
     8941 cgatcaaggg ggcggagcgg ggacggatcc accttttttt ggcgaccagc gaccttcacc
     9001 tgaagtacaa gctgcggatg agccgcgagg aggttctcga acgctcgacg aagatggtcg
     9061 cctatgcccg caacaagatc gacgatgtgc agttttccct ggaggatgcg acccggaccg
     9121 acctcgatta tctctgcacc gtggtcgaag cggtgatcaa agccggtgcg aacgtcgtga
     9181 atctccccga taccgtcggc tacgcctcga cgggcgatct tcagcggatg gtcgagacgg
     9241 tgatgaaccg ggttcccaac gtcgacaagg cgatccttgc cgtccactgc cacaacgatc
     9301 tcggcatggg ggtggcgaac accctgaccg gactgaacgc cggagcgcgg caggcggagg
     9361 tcacgatctg cggaatcggc gagcgggcgg ggaacgccgc gttggaagag gtggtgatga
     9421 accttaaaac caggggtgac atctatccct ttgaaaccaa catcaacacc gagcagatca
     9481 cccagtccgc ccgggtgctg aagcagatca ccggggtgaa gatccaccct tccaaagcga
     9541 tcgtcggttc caacgcgttc gcccacgaaa gcggaatcca ccagcatgga atgctggcca
     9601 acagcctgac ctatgagatc atgactcccg aaagcgtcgg ggtgaagaag acgagcctag
     9661 tgctcggcaa gcacagcggg cagcacgcct tcgaacaacg cctcggcgaa ctgggctact
     9721 ccttcaccaa agaggagttt tcacggctct tcaatgaatt caagaatctg gccgaccgga
     9781 agaagaacgt cgaagagcgc gacctcatcg ccctggtcga atcggctcat caaggttctc
     9841 cgacgatctg ggagcttgac tccttcgtcg tcaacagcgg caacctcatc acctcgacgg
     9901 cgtgcgtcac gctgaagaag ggggatgaaa aacaccagga ggttgcctgg ggaaccgggc
     9961 cggtctacgc ggccctcagg gcggtggaga agatcgtcga ccatccgttc agcctggagg
    10021 actatacgct gcaggcggtc accgagcatc gggacgccct cggcgaggtg ttcgtcaagc
    10081 ttcgggacga gcgggggacc taccgcggtc ggggcgtgtc gacggatgtg atcgaagctt
    10141 cgatcctctc ctgcctcgct gcgatcaaca agatggtcga cggcgctccg gccaagcggg
    10201 agcacccctt caagccgacc aacgaccatt cctttgaaaa cgacatgctc atgaacaaca
    10261 gcgataaaag gagattcatg tgaaggtagc gatctttgac tccaccctcc gcgacggcag
    10321 tcagggcgaa ggaatcagtt tctccgttga agacaagctg aaagtggttc gagccctgga
    10381 tgccttggga atcgacttca tcgaagcggg gaatcccggt tccaatccga aggatcagga
    10441 gttcttcgac cgcgctcgaa ccttggagct gaagcatgcg gcgatcgtcg ccttcgggtc
    10501 gacccgaaga aaggggatcg aagcgaagga cgatccgaac ctccaatccc tgatcggcgc
    10561 caagacgaag atcgtttcga tcttcggcaa aagctgggac ttccacgtga ccgaaattct
    10621 tcggaccacg cttaaggaaa accttgcgat gatcgaggat tcgatccgct tctgttgtga
    10681 tgccggcgtg gaggtcttct tcgatgcgga gcactactac gacggatatc ttgccgataa
    10741 ggagtatgcc ctcgaaaccc tcagggcggc gcttaagggg ggagcctcca cgctggttct
    10801 ctgcgagacc cggggcggga tcattccttc cgagtttgag cggatcacca gagagacgat
    10861 ggcggcgatt ccgaacgccc agtgggcggt tcacgcccac gatgatatcg gaacggcggt
    10921 ggcttcttcc atcctggcgg ttcaatgcgg ggtcggccag gttcagggaa cgctccttgg
    10981 gatcggcgag cggtgcggca acgccaacct ctcgacgatc atcggaatcc ttcagacgaa
    11041 gctcggctat gagtgcctaa acggcgagag cctggagttt ctgtatccga cctgtcgggc
    11101 gatcggggag gttgcgaacg tccggatcgc ccggtcgatg ccgtttgtcg gcaagtccgc
    11161 gttcgcccat aaggggggaa tgcacatcga cggagtggcg aagaatcccg cctccttcga
    11221 gcacatcgat ccgacggtcg tcggcaatga acgaagactg ttgatgagcg aggttgccgg
    11281 ccgggcgctg atgcttcgaa gaatcgccaa agtggttccc gatctggaca aggacgaccc
    11341 ggtgacggtg acgctgatgg ccaggctcaa ggagctggaa gcggtgggat accagttcga
    11401 aggggcggag tcctccttcg atctggtgat tcggcgtcat ctgaacctct ataaaccata
    11461 tttttccctg atccactatc agacgatcgg aacggctccc tacctggatc ccgactccac
    11521 ggcgacccat gcggtggtga tcaaggtatg ggtcaaagga cgcagcgcga tcgccgcagc
    11581 ggaaggggaa gggccggtga acgctttgga caccgccttg cggaaggtgc tggaggagtt
    11641 ctatccgacg ctcaagagcg tccatctgac cgactacaag gttcgggttc tggattcgaa
    11701 ggaagcgacc gcctcgacgg tccgcgtctt gatcgaatcg accgacggga cggagagctg
    11761 gtccacgatc ggggtaagca aggacatcat tcaggcgagc tggtccgcct taagcgacag
    11821 catcgagtat aaactcatca aggacgggat tgttcccaac ggtgagtgcg agaaggagat
    11881 ctaggcgtgg gcatgacgat gacacagaag atcctcgcaa aagcggcggg caaagcgtcc
    11941 gtccgagcag gcgagctgat catggcggac ttggacctgg tgctgggcaa tgacatcacc
    12001 gcaccggttg cgatcaacga gtttccgaag ttcgggaagg aaacggtctt cgacagggag
    12061 aagatcgccc ttgttcccga ccatttcacc ccgaacaagg acatcaaagc ggccgagcag
    12121 gtcaaagcga tgcgaacgtt cgcccacgat catggaatcg tcaactactt tgaagtcgga
    12181 cagatgggga tcgaacacgc cctgcttccc gagaaaggcc tggtgggcgc cggagaagtg
    12241 gtcatcggag cggacagcca tacctgcacc tacggggcgc tcggttcttt cgccaccggc
    12301 gtcggctcca ccgacatggc cagcggaatg gccaccggca aagcgtggtt caaagtcccc
    12361 tccgccctga agttcaacct gaccggaaca ctgaacaggc acgtctcggg caaagacgtc
    12421 atcctgcaca tcatcggcct catcggggtc gacggggcgc tttatcaatc gatggagtac
    12481 agcggagaag gggtgaagaa cctttcgatc gacgatcgct tcacgatcgc caacatggcg
    12541 atcgaagcgg gagcgaagaa cgggatcttt cccgtcgacg aggtaacgct tggctatctt
    12601 gccgaacact gtccccgcac ccctcggatc ttcgaggcgg acgaggacgc cgaatacgag
    12661 gcggtctacg agatcgatct ttcggcgatc aagagcaccg tcgcctttcc ccatcttcct
    12721 tccaacaccc gtccgatcga cgaggtcggc gaagtgaaga tcgatcaggt ggtgatcggt
    12781 tcctgcacca acggccggat cagcgacctg aggcaggccg cggcggtcct gaaggggaag
    12841 aaagtggcgc cgtgggtgcg ggcgttgatc ttcccggcga cccagaagat ctggaagcag
    12901 gcgatgaatg aagggctgtt tgaaatcttc gtggatgccg gctgcgccgt ctccactccc
    12961 acctgcggtc cgtgtctcgg cggccacatg ggaattctgg ccgaagggga gaaagcggtg
    13021 agcaccacga acaggaactt tgtcggacgg atgggacatc ccaagagcga ggtgtacctc
    13081 gcaagtcccg ctgtcgccgc ggcaagcgcc gttaccgggt acatcaccga cccgaatacg
    13141 atcgaggagg cataagatga gagtgtcagg aacagtattt cgctatgggg acaacgtcga
    13201 caccgacgtg atcattcccg cccggtatct caacacctcc aatcacgccg aactggcgct
    13261 ccactgcatg gaggatctcg acgcgacctt caaggacaag gtgaagaagg gggacatcat
    13321 cgtggccgac gcgaacttcg gctgcggctc cagccgggag cacgctccga tcgcaatcaa
    13381 ggagagcgga gtctcgtgcg tcatcgcccg ctccttcgcc cggatcttct accgcaactc
    13441 gatcaacatc ggcctgccga tcctggaatg ccccgaagcg tgcgccgaca tcaaggccgg
    13501 ggacaccgtc gttgtggatt tctccaccgg ggtgatcacc aacaagcgaa gcgggaacac
    13561 cttccaaagc gagccgtttc cccccttcat gcaggagctg attcaggaag gaggcctggc
    13621 gaactatgtg gccaagggag ggatcgcgtg atggaaaaga aacggatcgc agtcatcgcc
    13681 ggtgacggca tcggggtgga gatcaccaga gaagcgctca acgtgctgaa gaaaatcgcc
    13741 ccggacggtt ttgtctttga acaccacaaa gcgggaggcg aagcccttga tgcctttgga
    13801 gttcctcttc ccgaggagac gctgaatgga tgcaaacaaa gcgatgctgt cctgctcgga
    13861 tcgctcgggg ggccgaaatg ggacacggtc ccttcgcatc tgcgccctga aaaggggctc
    13921 ttgggtctgc gcggcggact cggcctgttc tgcaacctcc gtcccgcaat cctgtatccc
    13981 cagcttgccg gggcgtgtcc gttgaagaac gagctcgtcg aaggcgggtt ggacatcatg
    14041 gtggtccggg agctcaccgg ggggatctac ttcggagagc ggggccggga aggggattcg
    14101 gcgttcgata cggagcgcta cagcaaggcg gaggtcgagc ggatcggacg aaaagcgttc
    14161 gagatcgcca tgaaccgaag caaacggttg tgttcgatcg acaaggcgaa catcctgatt
    14221 tccagccagc tgtggcgcga gacgatcaat gccctctcca aggagtatcc cgaggtttca
    14281 gtgacccata tgtacgtcga taatgcggcg atgcagctgg tgaggaatcc ccgccagttc
    14341 gacacgatcg tcaccaacaa cattttcggg gatatcctca gcgacgaggc tgctcagatc
    14401 accggctcga tcgggatgct gccttccgcc tccctcggcg aaggaggggt ggggatgtat
    14461 gagccgatcc acggttccgc tccggatatc gccggaaagg atatcgccaa cccgatcgca
    14521 atgattcttt ccgcagcgtt gatgcttcgc cactccttcg ggatggagcg tgaagcgaaa
    14581 cggatcgagg atgcggtaag cggcgtgctt gatcaggggt atcgaaccgc tgacatcatg
    14641 agcgggggga tgaccctcgt cggaacgaaa gagatggggc gccgcataac cgaggcgctt
    14701 tgaggaggat gtgatatgaa acgtcgttca gcacagatga ccgaagggcc gcagcgctcc
    14761 ccccacagat cgttgatgaa agcgctgggc tggaccgatt ccgagatcca tatgccgatc
    14821 atcggagtcg tcagcggagc caatgagatc attcccgggc acatccacct cagcaggatc
    14881 gtcgaggcgg tgaagagcgg ggtccgccac ggcggaggaa accccgtcgc gtttccggtg
    14941 atcggagtct gcgacggaat cgccatgggg cacacgggaa tgaagtactc cctcgtaagc
    15001 cgggagctga tcgccgactc gatcgaagta atggccaacg cccatccctt cgacgcattg
    15061 gttttcgttc caaactgcga caaggtcgtc cccggaatgc tgatggccgc tgcccggatc
    15121 aacatcccct cgatcttcgt ttccggcggt ccgatgcttg ccggaaaaaa ggatggacgt
    15181 gagccgatca acctttcgcg gatgttcgag gcggtcggtt cctacgccct tggggcgatc
    15241 gacgatgccg agctgcttga ctgggaaaac aactcgtgtc ccacctgcgg ctcctgttcg
    15301 gggatgtaca ccgccaacag catgaactgc ctcaccgagg cgatcggaat gggccttccg
    15361 gggaacggca cgattcccgc cgtctatgcc gagcgggatc ggcttgccaa agaagcggga
    15421 tatcggatca tggagctgct caaagaggat gtccgacctt tggacatcat gacggagaag
    15481 gcgtttgaaa acgccctcgc cgtcgatatg gcgatcggtt gctcgaccaa cacgatgctg
    15541 cacctcccgg cgatcgccag cgaagcggga atcgatctgg atatcttcat cgccaacgag
    15601 atctcggcca aggtgcccaa tctctgccat ttggctccga gcggaaacga tcatgtggaa
    15661 gatcttttct acgccggcgg cgttctggcg gtgatgcatg aactgggacc gctcggcgtg
    15721 cttcacgggg atctgccgac ggtgagcggt aaaaccatcg gccggttgtg ggcggagcat
    15781 ccggtgcgaa accatgaggt cattcgaccg ctgaacaacc ccttcgacaa gaccggcggc
    15841 atcgccgtgc tgaaggggaa cctcgccgtc gacggggcgg tggtcaaacg gagcgcggtc
    15901 gatccctcga tgctggtcca cagcgggcct gcccgggtct tcgacagcga agaggaggcg
    15961 aacgaggcga tcttctccgg cagaatcaac aagggcgatg tgatcgtcat ccgctatgaa
    16021 ggccccaaag gaggcccggg gatgcgggag atgctcagtc ccaccagcgc gatcgccggt
    16081 atgggcctcg acaaggatgt ggccctgatc accgacgggc gcttcagcgg agcgacgagg
    16141 ggttcctcga tcggtcacgt cagtcccgag gcggcaagcg ggggcctgat cgccctcgtc
    16201 gaggaggggg atgaaatcct gatcgacatc ccgggtggag agttgacgtt gaaggtggat
    16261 gaggcaacat tgaacaaacg ccgtgcctcc ttcaaggaga aagaagtcgt tcgctacggc
    16321 ggctacctcg atcgctatcg aaagcaggtg accagtgcga acaccggagc aatttttgcc
    16381 caggagtgac ggatgaaatt acgtggtgcc gaaatcattt tggaatgtct gcttgaacag
    16441 ggcgtcgata cggtcttcgg gtttcccggc ggggcggtgc ttcccctcta tgatgccctc
    16501 tattcctatc gcgatcgaat caaccatatt ctcaccagcc atgaacaagg cgctgcccat
    16561 gccgccgacg gctacagccg ctcaaccggc aaggtcgggg tggtgttcgc aaccagcgga
    16621 ccgggggcga ccaacctggt gacgggaatc gccaccgcct atatggacag cgtgccggtc
    16681 gtggcgatca ccgggaacgt gacggttccc cttctcggga aggattcctt ccaggaagtc
    16741 gatatcaccg gaatcacaat gccggtgacg aagcacaact tcatcgtcaa ggatgtgaac
    16801 gatcttgcca ggacgattcg cctggcgttc caaatcgccc aggagggccg gccgggcccc
    16861 gtcctgatcg atgtgccgaa ggatgtaacg gtcgagtacg ccaagtatga gcgtcaagtc
    16921 atcgagccgg tgaaaccccg gtcggatcgg atcagcgaac aggcgctcag cgccgcgctt
    16981 gagcttattc atagcgctga gcggccggtg tgttttgtcg gcgggggagt cgttcgggct
    17041 cgggcgagca aagagcttac cgagttcatc gaaaagatcg atgctccggt gtgcgcttcg
    17101 atgatgggga tcggagcgat tccgacaacc agcacacgct atacggggat ggtcggaatg
    17161 cacgggacga aggtctccaa catgatcgtc tcgatgtgcg acctgctgat tgtgatcggc
    17221 tcgcgcttca gcgaccgggt cgtcagcaaa gcgtccgcgt tcgcccgggg agcaaacatt
    17281 atccacatcg atgtggaccc ggcggagatc gacaagaaca tcaccgccac cgtcggaatc
    17341 gtcggggatg tccggatcgt tctgaaggag ctcaaccgaa gaatcgacaa gcagattccc
    17401 tccaatgggt ggatggatca ggtgtattcc tacaaagaac ggtttccgat ccggatcgaa
    17461 agcgagaacc agcggagcaa ggaggtcctc aaggcgcttc agaaggttct gcctgaaggc
    17521 gctttcgtgg tcaccgaggt cggccagcac cagatctggg ccgcccagtt cctcaagcat
    17581 acgcagtgcg gtcacttcct caccagcggg ggcttgggaa cgatgggcta cggaacgggc
    17641 tctgcgatcg gcgctcaggt gggcaacccc gaagctcgag tggtcagcgt agccggcgac
    17701 ggttccttca agatgaactg caacgagctt gccacgatcg cccggtatcg tttgccggtg
    17761 gtcattctga tcatgaacaa ccggaccctc gggatggtcc gccagtggca aaccctcttc
    17821 ttcaattcac actacagcga aacgacgctc gacaccccga tcgactgggt gatgctggcg
    17881 aatgcgtatg gtctcaaggg aatgcggatc gccaaggagc aggatcccct tccgattctc
    17941 aaggaagcgt ttgcgctgaa tgaggcggtt gtaatcgatt gcgagattcc taccgatgat
    18001 aaagcctatc cgatggtcgc cccgggagct tcgatcgaag agatgcttgg ggtgaaggag
    18061 tcatccaaat aggagacgaa ttccacgaga gatactccca gcccgttaca gatcgcaaag
    18121 agggtggtca ggtgaattcc ccgcctgtcc gcttcgatat agtaaagctg actgcgcgtc
    18181 agctttgctt tttcaatcag ctgctcctga gtcatgtttt tttcatagcg caggtgcttg
    18241 attacttgtc cgagtgtcat tgttcccatt ccgatagtat gtcaaagaaa aaatgaacct
    18301 gacaggttta tggcataaag tggtgatgta tgcccgaatc atcaaaattg ttgtataatt
    18361 agctaaatag ttgactgata tttcctaaat tccgtcagat atcgatggat atttcccaat
    18421 aatcaatctt ctcttggtat actgaagcat ctacccaagg aggtgcggat atgggtgcgt
    18481 ttaatcatgg tgaactgacg gtgatctgta cgaatctgcg gaaggcgtgc gagaaacagc
    18541 ttcgaagtga agaagctgca ctgtttggca accttgcaac gtatttttca gggaaaacgg
    18601 aatgtcccgg acaggcgacg cttcaagaga tcggagcggt catcgatgag gagcttgcga
    18661 ccctctatcc ccaggcgaat gagcaagccg aagcggccgg ggatcgggga gcgaagcggg
    18721 cgctgttgtg gggggagaag gtcacccgcc tgcacaagag ttttttgatg cggcttgagc
    18781 gcaaccccaa gcttcttgat gaaactccgg tgtgggtgtg tgaaagctgc gggtttctct
    18841 tcgtcggagc ggtttctccg gcgatctgtc cgatctgcaa agttccgaac ataaaaattc
    18901 ataagatgga ggtcagatga tggttgcttc ccgtaattta cgcttatgca cgaaggattg
    18961 tatctgcctc tttgtctgcc cgaccggggc gaccgatacg gaaagcggtc agatcgatgc
    19021 ttcaaaatgt ctggacgggt gtcgcctctg cgttgatgca tgccccggcg gagcgatcta
    19081 tctggttccc aggaactatc ccaagccgca acccaaacgg gaagatgtgg tcaaagccct
    19141 ctttgctctt gcggaatcga aggtaaaaac acgagcgctg gccaatgggt tgggggacga
    19201 ttaccctctt gcccgaaagg ccctcgattt ctccttggag gttctggcgg aggattgtgt
    19261 ccgcgagggc gggtatatgc tgccgcagag cgctgtaaca aaagagttcc tcaagaatct
    19321 gaaggaaagg tatggggacg atcccgcctt ccccgtcaag gcggttgagc gactgcttga
    19381 tctgttatag aacggcttcg atcgccaggg caagcccgtc ttcggtgttc gggcgagtca
    19441 caacgtcggc catctccctg atgtcatcgg gagcgtttcc catcgccacg ctgtaccctg
    19501 cggctttgag catcgccacg tcgttgtaat aatctccgac cgccatgatc gcttcccggg
    19561 gaagtgcgta atgggatccg agggctttga ccgcgtcccc cttgtccact ccccggtgga
    19621 agatctcgag gtagatcggc cccgatcgga ggatggtgaa ccgggaaccc atctgctgga
    19681 tcagttgtcg ttccatcctg gaaagatact ccgggtcttt gtccatgcag aggactttga
    19741 acggcttttc atcggttccg agattccctt cgatctcata gaaggagcgg acgattcctt
    19801 tgaacccact gattttcagt tcgtattccg tccaatatcc ctctgttgag gcgtaccagt
    19861 tggtgttcgt atagacaaac gcgttcagat cggtctgttt cgcccattcg aggatctgat
    19921 gaatctcgtc cttttggagc ggatgttcca ggatcgatcg gcccttctcg tccaagacaa
    19981 gcgatccgtt gagggtgccc agcgggccgg tgatgcccag ctcttcctga atcttcacaa
    20041 ggctctttcc gattcgaccg ctgaccaacg ctacgatgat tcccctcttg tgggcttcct
    20101 gaattgccct cttcgttcga gggctgatcg tgtggtcggg ccgcaggagc gttccgtcga
    20161 tatccgtgca gataagtttg atggagggca tgtctttacc ttagagaaat agtcggtctt
    20221 tgcatagtcg gaaaaaggtg tttctaaccc cacaccctgc ttgccagcga tattccgatc
    20281 acgaggatcg ccgtgatgat gatcaaaacg gaagagctcc gctcttgctg attgttggca
    20341 gatacctgca ggcttccttc cgctgcgcag aagagcgctc cgtcgacccg ttccagaagg
    20401 gagcctttcg ccgtccggaa gcatgaagtg aggattccga agtaggagag ggccagcacc
    20461 aggagcgaac cgagtctgcc tttgaggcgg gggttggagg aaaccaggct ttggctggcg
    20521 gagagcaggg cgatcagcct caggcctttg gtcagggcca aggcgatcga gcctttcgtc
    20581 aacgccaagg ttccgacggt aaagatgact tggccgttcg gctcaaagag ggagaggacg
    20641 attagggagg aaaggagcat cagcggcggg aagaggcgga tcttccgacc cgatatccct
    20701 tgaatcacat acatcaggac ggcaattccc aaaagcggaa tcggaccttt ggcgaagagg
    20761 atcgctccga tcgcgatgat cagggccacc gttcgttcga ccagatgggg gcgctcctcg
    20821 gcggtcggca gaaccggctc aaaggagtcg gcaaggaatg cggcgggaaa tgatccatgg
    20881 cgcaggtagc gctcaccgaa aacgccgagg atgatcgagg tgatcatccc aagtcccagc
    20941 atcaggggtg ccgctaccca gatcgccggt ccgtacacga tccaggaagc gacctgaagc
    21001 tgggaaagat tgctgacgaa cgccccgacc acgctgatgc cgataaggga cacccgttct
    21061 ttcagcaagc ttttcaggag tttcatcgca tacccgctgc tcatcgttcc ggcgagcgag
    21121 atcagaaaga ggtaggagaa gagggttccg ctgaccattc cctgaccgat cgccttcaat
    21181 aaaagaataa agaagtagga ggggtaatcg atcgattgga ggatgagaag caggggcaga
    21241 ttggccagtc cgaggcgaag aaagggaagc ggtttgggga tgagatgctc cagggaggaa
    21301 agtaggaggg tgctggcggc gatgaaggag atcttccggt cagtaggcga catcatcgat
    21361 ctcctggggc atcggaccga ccacggtgag caggacgtgg ttgggcagac aggcgatgaa
    21421 cgagccgctt cgatcgatcg ggctctggaa ggtgcacgtc ttgttggggc acgccgagtc
    21481 gacgatccgg gctttgcctt cccggatctc gatgagggtg tcaccgaggg gccctgttac
    21541 ggtaacggat cggtcgttct tcagcgaata ccggtagacg ccgtcggcgg tctgaacgga
    21601 cacatagccg ttgccggtgt tttgccccag ggcaaagacg aagaagccga cggcggcggc
    21661 acagagaagg ataatgacaa gatcgcccac cagcgagctt ggaatcttca tgtcttgagt
    21721 gtacagagct cattcgagga gggcaatatg aatccgatcg acaattgacg gaagaccggg
    21781 cgcaaattat gattgtgtag aatatgtgta gttaggtgtt gaatatgcaa aggctcttgt
    21841 gaaagagcac aaaacgtgat gaggtaggct atgaagttga tatacgtggc ggacggctgt
    21901 aacgacgacc gcgaggcgat gaaacaatat ttggaactct cgggtttcga ggtacaggcg
    21961 tttgaggagc ttcacggcct tcaggtggca ttgaacaagc aagccccgga tcttctgctt
    22021 cttgaagtgc accacagcga cggcgacggc ttcaccttct tgaagaagat gaagcagagc
    22081 cacaccttcc cggtgatctt cgtcacaaac agaagcgccg agagcgatcg gatcttcggt
    22141 ttcgaactcg gggcggacga ctatgtgtgc aagccgtttt ccgccaagga actgaccttg
    22201 cgggtaaagg cgctgttcac ccggatcgaa ttgagctctt ccagaaagga tcacgccacg
    22261 tactggaccc tcggttcatc ccgcctccag cttgatgagg agagccacat cttcagcctt
    22321 gacggggagg tgatcagtct gaccacgatg gagtggcgga tcatgagcta cctggtcgca
    22381 aatgcgggta tcctgatcac gcgttcgcag attctcgaac actgttttga ctacagtttc
    22441 gaatcctacg accgcagcgt cgatacccac atcaagaata tacggaagaa gatcggaccg
    22501 atgggcaccg aatggattga aaccgtgcgg ggatacggct atcgctttgg cgggcagtca
    22561 attccgcacc atggataaca agagatgaaa ccgcttattt acatcgtcga agaccatgca
    22621 gtcatccggg acggggtgcg ccagtacctc gagctgtccg gctatgacgt gcgcgagttc
    22681 ggagatctga cttcaacgaa gaaggcgctc caaacggaaa cccccgatct gctgattcag
    22741 gatgtgatgc tgccggacgg ggacggattt ctgttcatca aggagctgaa aaaagaaaag
    22801 gatattccgg tgatcttcat gaccgcccgg atcgaggaga gcgaccggat cctcggcttc
    22861 gagctcggcg cggacgatta cgtgaccaag ccgttcagcc cgaaagagct gatgatgcgg
    22921 gtccaggcgg tcctgcgccg gacaagcggc ccgcaaagcg atacctttta tatcggtgat
    22981 gaagtcctta cctttgacca aggaactcac caggcgcata taaacgggga gccgatgagc
    23041 ctgaccgcag ccgaatggcg ggtgctcgag tacctgatcg aaaacctcaa tcaactgtgc
    23101 acccgggagg atattctcaa atactgtttc gactacacct acgagtcgta tgagcgggtg
    23161 gttgacaccc acatcaagaa tatccgggcg aagctgggcg ggggcgactg gattgagacg
    23221 atccgcgggt acgggtatcg cttcctcggt tccccaaaga aggaaggggc aggatgagaa
    23281 aacagtttat ccgcctcttc ttcggctttg catccgtggc gctcttggtg ctgctcatnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc aaaccggcca atcgcaccac
    23461 aagacgggtc cgaacccttt cctatacgat ggaactcaat gctcagaccc cctcactgag
    23521 ggaggagcga acgatccgca gtcaggagat tccgctctct tcggagatcg ggccttcgga
    23581 tatcgccgga accttcaccc tcacggtgaa cggggaggag gtcggctaca tcgatgtgtt
    23641 gacgtatact ccgttcacct atcagagcac gtccttcatc ttgaaggcgc tggtggcccc
    23701 gttcgtctgg tcgatccccc tcggtctgat cctggcgctc atcctcgggg ccgtgatcag
    23761 ccgaaggacg cagcggtaca gcgacggaat ctcttcagcg cttgaagagc tgagcagggg
    23821 agaacacaac gtcgagctgc ccgaggcgaa gttcgatgaa caggagacga tcaacgcgtc
    23881 gatccgggcg ctcgataaga ccctgctaca caacaagcag tcccgccagg cgtggctccg
    23941 ctcgatcagc catgatttca atacgccgtt gacggcgatg gggctccttc tggaggggat
    24001 ccgggataag gtattccccc tttcacccga gacgattgca aaggtgcagg gcgagcacgc
    24061 ccttcttgcc tctcgaatcg cccaggtctc gctctatgcc caccttctcg gacaggaacc
    24121 tccgattgat gaaagaccat tcaatgccca tgagttcgca aaggagattc ttcaatcaac
    24181 aagaagcgaa aggattgaac tcacagtgaa taccgaagaa ctcatcggcg accggacgtt
    24241 gttgggctat gcggcaaatg ccctgctttc aaacgccctc aacgcttccg gggagccggt
    24301 ccgatggacg atcgattccc gttcgatgga gttcaccaac aagggaacgc tgccgcagga
    24361 gatcgatttc ttcgagccct gggaacgggg ggacaaggcc cgaacatcca gcggcagcgg
    24421 gatgggattg ccgatcgtgt atcagattat gcgccttcat cagggcaatg cgacgatcga
    24481 gcagcaaaat gatgatgtaa aatgcgttat ttcatggtaa aaccccaata aatgggtgaa
    24541 acaaggtaga tttcgctaga ttcttataca cacatagagt atactctgta ggtaagtcat
    24601 gggatcaaac cttccctggg agagatggat tgagggtgtg gcctcctcaa ccacttggaa
    24661 aagtgcgagc tttttcaagg cgatcgaggt aaatgtgtat atcattctta tatgattgtg
    24721 aaacagctta tgtacaggaa taaagttgac aaagtaatcg atagtgtctt tgtaaccttc
    24781 ttcaaaagca caaataagca gaatgtgttc aactgctcca cacactctgc gtatttgtac
    24841 acacaagctt cacgcatata catcaaacct cacgtaaata cattgtccct acaatgtctt
    24901 tacattcaac tttcaatgta catcagcata ctgatgttac aaataaactc tcacgcaaaa
    24961 aaatggagat ttacgtcatg aagaaaaagt ttttaacttt ggcgctggtt ctcatggttg
    25021 cggtaacact gtttgctgcc accccaattg aggtcagcgg atcattaacg gccggttata
    25081 agttcacctt cggcggtgcc acgcctggaa ctgttgggaa taataaaggc aaagctacca
    25141 tcgtctcatc cttcaccggc gatcattgga aagtatcaat cggtggaaat cttgcgtatg
    25201 gtgctaataa gggacttgct agtgctactg ctgaaattta tctcgacaag gccttgaaag
    25261 accaaggcgt cgacatgggc gatttcaaag tgaccctcca tgccggcacc ggtgtcgaaa
    25321 agggtgcgta ctccgtgttc gcgaacaagg gtgcactcgg caaggaagcg gccgcgatgg
    25381 ttacgaaaga caacgccggc atcacgctcg gatatggctc cctcgcgaaa ctgtacttcg
    25441 ccttcttccc gaacaaggcg ttcccgatgg tcgtctctgc tcaggttact cccgttgccg
    25501 gagtcgacgt aaccttcggc tactcgaatg agagcgcgaa caacgacctc ttggttaccg
    25561 cgaaggctga cattgccaaa ctcgccgact tggattttgc tcttgcttcg accgcacagt
    25621 tcgcctatga cgtcgacgcc aaggccatgg tcgtcggtgt cgatgcatcg accacgatcg
    25681 aaggcatcgg tg
//
LOCUS       DAOSLI010000026        25456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2168_length_25456_cov_32.957915, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000026 DAOSLI010000000
VERSION     DAOSLI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 25456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25456
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_25456_cov_32.957915"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   49..930
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=49..76
                     /rpt_unit_seq="gtcttccccgcatgcgcgggggtgtttc"
     gene            complement(935..1816)
                     /locus_tag="PLF67_05260"
     CDS             complement(935..1816)
                     /locus_tag="PLF67_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOE89455.1"
                     /translation="MSKSTLATTVARINLISNAFLAITKILIGYFAHSAALLNDGINN
                     LGDTLSSIITMVGISAGSKKADDDHQYGHERLECVAAILLSGIIMFVGIALFGSGISS
                     IIKKTYLETPVPTFITVIASLASIIIKGAMYIYTQWAGKKVGSPALMASAGDSLSDVF
                     ATSGGLIGILFSRMGYPIGDAVAAVVIAIFIFRVGVEIFRDGMNQMVDRACDESFTKE
                     VRALIEAQEGVLKVDLLRTRQFGSRCYVDVEIAADPNQTLTEAHSIAERVHKTIEASF
                     PTVKHCMVHVNPSRKKQ"
     gene            complement(1851..3644)
                     /gene="aspS"
                     /locus_tag="PLF67_05265"
     CDS             complement(1851..3644)
                     /gene="aspS"
                     /locus_tag="PLF67_05265"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOE89456.1"
                     /translation="MAEHFSKRTATCGSLTKADDGKTVVLNGWVHRHRDHGIIHFINL
                     RDRYGITQVIVDDDSSKELQQAALSLNLEDCIAVTGVVRARPASMVNPEMATGEIEVK
                     AEKIEILSTCAPLPFQIDDGNVPREELRLKYRYLDLRTEGMKKRIKLRHDMVWAIRRY
                     LSARGFYEIETPTMIKSTPEGARDFLVPSRIHVGKFYALPQSPQLFKQILMVGGMDKY
                     FQIARCYRDEDARGDRQLEFTQLDLEMSFVTRDDVLSTMEGLFGYVFKEVMDYDLPPT
                     FRRFSYEEAMNTYGCDKPDLRFGLELQDMNDLASRSTFSTFKEIVAQGGHVKALCAPH
                     SEIVDFSRKYITDLENAAKVYGAHGLAWMKVGADKTASGGVSKFFEGIQGEVIDRFDA
                     KEGDMILFVAHNEWKKTCASLSAVRSKLGKDLQLIRPGFEFCWIVDFPLFEYNEERDQ
                     WEAAHHMFSMPQAEYIDTMEEDPGRVKGDLYDLVLNGYEVASGSIRIHDVELQKRIFR
                     ICNFDDETAQERFGFLLTAFSFSPPPHGGIAPGIDRIAMIMAGESSIHEVIAFPKNTA
                     GISPMDDSPSFVEEKQLEELHLKIVYPEKKE"
     gene            complement(3919..4788)
                     /locus_tag="PLF67_05270"
     CDS             complement(3919..4788)
                     /locus_tag="PLF67_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOE89457.1"
                     /translation="MLKDTKANKLFMIALVILLALGFVWVRGEVFRGKTIRDTEAIPF
                     AKGDSLSVSAVSNPIVLEINPEATSVSLEIGRIDKKQLAVAKNGSNLSIKVSPKKRIW
                     IFPVFFGSPSSTLVVTAPPDRIKDLSLASISGKVTVLNSLDLNSAEISSTSSKVSVLD
                     ITANKTISLSSVSGSVDAGDLTSNGPIEVSSTSGRIEVESLRAPKITVNSVSGSVDVA
                     LPLPSPSDVTLSSTSGQVEADLLGADHYTINAHTTSGSITIGGEQRSTLTKGDGSSRI
                     TLQTVSGSISVRP"
     gene            complement(4790..5134)
                     /locus_tag="PLF67_05275"
     CDS             complement(4790..5134)
                     /locus_tag="PLF67_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOE89458.1"
                     /translation="MATRKLYRSPNGKVFGICAGIAEWRDLNVDIVRLIVGISILTTG
                     IFPGALIYLGAALIIPMAPGGRTFEQRHGETVSDSELKSKYENLKKKVEEMESEVLNK
                     ERDWDDRFFSGK"
     gene            complement(5147..5485)
                     /locus_tag="PLF67_05280"
     CDS             complement(5147..5485)
                     /locus_tag="PLF67_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89459.1"
                     /translation="MTAVPILAVIFTFVIILNVLENSSKERNRKIEAALRMREMELGY
                     PPGTYSSPKWSKKFSNKDAEKSIRETYEQYASKGSTRVEMEQGIVDLEERLNNLEEIL
                     RSRKNSYNKE"
     gene            complement(5482..6129)
                     /locus_tag="PLF67_05285"
     CDS             complement(5482..6129)
                     /locus_tag="PLF67_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOE89460.1"
                     /translation="MGVFSRFLDIVNANINALLDKAEDPEKMIRLMIQEMEDTLIELK
                     SSAAAKMATLAKSKREYNEFEEEMKRWQARAELAISKGREDLAREALLAKRQVSERLE
                     PLLSQIASSEELISVAKSEIDQIEQKLASVKQKYQTMVDRANRAKEEEVVNTTMRRST
                     DDRFAEMQDQIDRMQASNELNRRNASLDEQFRKLEEMEELEAELDELRKLAGGKK"
     gene            6293..7270
                     /locus_tag="PLF67_05290"
     CDS             6293..7270
                     /locus_tag="PLF67_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE89461.1"
                     /translation="MAAQERYSQQPLGESEVFLDFQAKLSRVAAINRPVLLVGERGSG
                     KEIAARRLHYLSPRWDKNLVTVNCAALPPSLIETELFGYEQGAFTGAQKTRKGRFEEA
                     EGGTLFLDEIGLIPLEVQEKILRVVEYGTYERVGSSVTHEVNVRIIGATNADLPALCE
                     EGKFKQDLLDRLSFEVLFLPPLRERGEDILTLAYFFASKMAQECDREGMVTFTDEVID
                     KLMAYRWPGNVRELKNVIERAVYRSEDNVITELQFDPFANPFEEKTGQSKEAELVLGN
                     FEADRIEFEIRYLAEALKQGEGNQKEAAKLLGLTYDQFRGLYRKYQDRL"
     gene            complement(7324..8004)
                     /locus_tag="PLF67_05295"
     CDS             complement(7324..8004)
                     /locus_tag="PLF67_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOE89462.1"
                     /translation="MQFFKRVRDIVSSNVNSALDKFEDPQKMIALMVTNLEELQTKTR
                     SSIAGKKAELIALNHRVKEAQDAVNRWAERAKLAVANGKEDLAREALVEKRKAQEHHD
                     QLAEQITNLEGIIAAQTTQLAQIGDKLKEVKDKQQILVQRSISARERRQVAAVLRESD
                     SSNIASKFSELEAKIERMEAEAEMAAYSGSTSVLEQFTQMETDEAIEAELAELKKSSA
                     KKTPAKTE"
     gene            8215..8931
                     /gene="rsmI"
                     /locus_tag="PLF67_05300"
     CDS             8215..8931
                     /gene="rsmI"
                     /locus_tag="PLF67_05300"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOE89463.1"
                     /translation="MSTLYIVATPIGNLADMTYRAVEVLTNVDIIACEDTRHTQNLLN
                     HYKISKRLIATHAHNEDNSAKGIIGLLEEGKDVAFVSDAGTPGISDPGSRLVERVRSS
                     GFDVVPIPGASAFTTLVSVAGYIGRSIHFEGFLSPKSGRRKKRLEELLQRDESFVFYE
                     SPFRVVKVLEELSTLEPGRRVVAGREMTKKFEEFLTGSVEEVLDSLKGRQTVKGEFAL
                     LVAPKGVGASDDYDQETEEP"
     gene            8900..9673
                     /locus_tag="PLF67_05305"
     CDS             8900..9673
                     /locus_tag="PLF67_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOE89464.1"
                     /translation="MITIRKLKSLKEDTRLRKATLLLKELSRLGEIDSSYVKDLLQII
                     KESRAGGDKRVVGLIDRIGGQEGRARAFSLEDLHYRLLDLLGGQTADWDFVDEETSLD
                     IGQRVVCERYLVVDRIRSPFNLGSIFRLADSFGIKKIYIVEGGAEPTHQRTIKVGRGT
                     VETVEYEVVSEDSLLAGLKNSELPLFALESGGVDITEFDFPLAGICVIGSEELGVSPA
                     LLDLCDKRSGRVSIALGGTKGSLNVTSASAIMLHGWFVR"
     gene            complement(9676..11700)
                     /locus_tag="PLF67_05310"
     CDS             complement(9676..11700)
                     /locus_tag="PLF67_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE89465.1"
                     /translation="MSERTSQVLPLSFEQASFEFDPALIKRLRNQRLADRIVGQPRAL
                     RSLEMGLSLPKAGYNIFVSGESQSGRHAAVRHAIEQVQDDLSGLRDIVYVCNFTQPDS
                     PHVLTFAPGESSRFIDSLEQFNHSITLLSEESETFLSNALTLVDSLIAQFPQKELERY
                     FFGLKGDITRQDAHIRRLGKADEALATRYLGNLVVDHSRSTKRPMIIESHPSMGNLFG
                     TIHAKDKPAHLSYHPGSLLESCGGFIIIDAAELFSKEGLWEALKRYLDATNLAQKEGN
                     VVKGELANSIIRPQIPPLSIKVILIGSEELYDALSEKDDRFLSLFKVNAQFDYTMNLT
                     EETIGQTIANIEHVVSEKGLLPITDDGLAQMLRYSAWFVESRLHLSTQFPILYDLLDE
                     ANYWAKQERKEMIDRSMILKVNEERSYASGIYQEKINEQIKNGEVIISLQGAKVGVVN
                     GLAVIDQGAYSFGTPTVISATVAPGTEGIVNIEHEAGLSGEIHDKGLLILEGYLRKHY
                     ARSFPLSIYAGIAFEQSYAEVDGDSASSSELFALLSAIGELEVRQDIAVTGSVNQMGE
                     IQPVGGINEKIEGFFHTCRFLGLTGKQGVIIPYQNVSNLILSYEILQAIRDRIFFIYP
                     IRTIEEGMRILTGRPSGVRNAKGNWSAGNFNYDIENRLKRMYQAGLSGRN"
     gene            11726..12496
                     /locus_tag="PLF67_05315"
     CDS             11726..12496
                     /locus_tag="PLF67_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOE89466.1"
                     /translation="MVSTDGMMRSELVAYLDEYLGIESFLSSDSSLNGLVVGGEDKRI
                     GKIGYAVDACQASFDQAAEEKVDFLLVHHGLFWGVPLAVTGSHYTRLATLIKHGIDLY
                     AAHLPLDAHPEIGNNIRIAKALDLEEIEPFAPYKGKTIGYKGRLQEPKTLKEIAGHLG
                     YEKTILLPFGKERIRTVGIVSGGASRNVSDALNDGLDLYITGEVEHESYHVALEGGIN
                     VLGGGHYVTEVYGVRALAEHLCERFGLEAVFIANPTGL"
     gene            12510..13028
                     /gene="lspA"
                     /locus_tag="PLF67_05320"
     CDS             12510..13028
                     /gene="lspA"
                     /locus_tag="PLF67_05320"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOE89467.1"
                     /translation="MAKTWRPLILTVFIVLADQLSKAWVVAAIPENTVGYRFFGDFLA
                     IVHVRNTAIAFSMGSALPIWIRLIFFVGLPVVLLVIVLLIYFNEHIPLSMPTRWTLAL
                     FMGGGIGNLIDRIFRSLRVVDFISVKFYGIFGLERWPTFNIADASLVVSGILLAVLLL
                     LEKEVDRDGKER"
     gene            13012..13299
                     /locus_tag="PLF67_05325"
     CDS             13012..13299
                     /locus_tag="PLF67_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide processing enzyme"
                     /protein_id="HOE89468.1"
                     /translation="MGKKGNTALFMIIATLVNILLMMILFIIGFFLLVRFVDPESSMV
                     PIYLALVFFFAIGGSFFLYSKLVTWASKRFNLEDKIVPLFGNRRRKPPKRD"
     gene            13302..14318
                     /locus_tag="PLF67_05330"
     CDS             13302..14318
                     /locus_tag="PLF67_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89469.1"
                     /translation="MPLSIAAYAKLSWYPFSDEPVINSSWYMNRLGDPSILLPGESPD
                     KKWHLFGHTFLGIVHFISENGISWEPRKMIELRGHSPSLYMEDGMYYLIYEKHDAIPS
                     QIDSIFARRKRKDRVSHSRIEMRSSADLILFSEPKIILDSREIPFAADGLGRPRVSRP
                     QLFKDGGVYTLFFGASHIVLEDSRHKSSRYFASATSSDLLGPYRLANHGRPLIEPDGD
                     DPNRSLAVGSVRVLKASDGYVALQCGKYWDRELKKSSTSLIQLESVDGVNWLPSTRPR
                     ILSPPSEGWASRYIISCDARYKEDEGCVYCYFSASSKRGFRPVKEEIGLLLGKDPVLR
                     KVFL"
     gene            complement(14315..15676)
                     /gene="rsmD"
                     /locus_tag="PLF67_05335"
     CDS             complement(14315..15676)
                     /gene="rsmD"
                     /locus_tag="PLF67_05335"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOE89470.1"
                     /translation="MRVTGGLYRGRKIVCPPGVIRPAMDRMRESMFSILGNQEGTAWL
                     DLFSGSGSIGIEAASRGAGLVHLVERDRGKKETIMKNIAFVETPIKLFISDVFRFLTQ
                     AKIAYDVVYADPPFPMEEKIRLLEAVERNKILKAEGLFIIHYPVEEKAQWPEQLGDLL
                     FIDERSYGRSTLRFYKRKKKMNHNDPVAISAENIASLRFTTYSFETDSLGAAQTAFYL
                     RIVQEAAGLHAAMRGCSIMDLHAEGKTWVITRNRIEIFGYTRWNEQIHVNTWAQEPIR
                     LHLPRIVEGYASDGSPLFQAKTFWAVIDLKNSRPLRPQEMNERIGLLKDSSRAIDMNL
                     PKSTVDFETLHQLGSYAPRVTYLDTDRNQHINNVSYLSWVLEALPAKFRTTYKVRSAD
                     VLYQKQTFLDDLLFVHTYAADEEGYNEETPSLVSTIVRKTADGAEEIVFTAHTEWKKR
                     SEF"
     gene            complement(15719..17794)
                     /gene="recG"
                     /locus_tag="PLF67_05340"
     CDS             complement(15719..17794)
                     /gene="recG"
                     /locus_tag="PLF67_05340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOE89471.1"
                     /translation="MTYLRTITDPVAKLGGVGPAATRSYAELGIHTQSELLLLAPRTW
                     EDRSTVRPLGSVQDGQVANTLVEVLSHSYFGLKKGMKRTLKIIVRDVSGLGDGRLSLL
                     CFGRNFLEKTIKPGRIYYLWAPVAIKGSEKQAVQFELHPATEDGDFPPQFRKILPVYP
                     LRGSLTQRLIRANIRQVLTRCGAFINELPESLMKELDLLSTDEAIRTYHFPPNLQQLA
                     RARRTLAFTELFYLQLVARRKPRKKPAKPAIPGDLELRLIERLPFALTNDQLAALKEI
                     REDLGAEEPMNRLLQGDVGSGKTLVGWISALKIIAEGGQVAFMAPTELLARQHAESGA
                     ALLEPLGVRLAFLTGSVKPKERSYLLTALQRGEIDLLVGTHALFSESVTFRNLRYVII
                     DEQHRFGVEQRLALLGKATEPHLLLMTATPIPRTLSLTVFGSLNISTLQTMPPGRKPV
                     ITLLVREESRIRMYKSVGVEFTRGHQAYFIYPRIDDSGQSDLRDVAGMYEFLKQQYPG
                     VPSALIHSKLDEEEKVAILEAYRKGELSYLVSTSVVEVGIDIPNATCMIIEHADRFGL
                     AALHQLRGRVGRSALQSYCFLVFSDELTEEAKKRLQVMKDSTDGFHIAEQDLIIRGPG
                     ELTGRAQSGYLNLAFASLSDDLELIEQARAASDRILQVDSGFLAPENTIIRSVLSEAP
                     PFEAGRSEA"
     gene            complement(17797..18408)
                     /locus_tag="PLF67_05345"
     CDS             complement(17797..18408)
                     /locus_tag="PLF67_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOE89472.1"
                     /translation="MQQTPTVLMTIANLLASLLSLYSLLIWIRIILSWLKTGTENPLA
                     YYLGKIVDPYLSWFKGIRSLVRPRFDLTPLVALAVLSVIQSLLRLFGSYGTITVTMVI
                     ALLLQTLYSYIVHPILFFFFILFVVRLYFCYKRSIHSITYIALLDSLIGGLLDWTHRT
                     FYARQTVNDRKLVISSLVVLIIAWIGSSLLLRLLLSLLTKTGI"
     gene            complement(18442..21900)
                     /gene="dnaE"
                     /locus_tag="PLF67_05350"
     CDS             complement(18442..21900)
                     /gene="dnaE"
                     /locus_tag="PLF67_05350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOE89473.1"
                     /translation="MMTRSFSGGFVHLHNHTDFSLLDGAAKISAYMKKAQALGMDSIA
                     ITDHGNLFGALHFYYAAKKVGIKPIIGCEFYVNPSDRTERPEPGSGKKSQQYHLVLLA
                     MNQIGYQNLMILSSLSYTEGFYFRPRIDDDLLERYNEGLICLSACLGGEILQHLLNNN
                     YEEAKRRALWFNSVFDDGRYYLELQDHGLEEQKRTNPLLKRLSDETGIPLVATNDIHY
                     IEQSDANAHDLLLCVGTNAKKHDPNRMRFPNDQFYLKSKEEMASLFSWVPEAIENSVE
                     IAQRCNLEIQFPGPQLPVFEVPSGYESPAEYLRSLSREGLANRFEVVTEKLTERLEYE
                     LEVIISMGFEGYFLIVMDYIRWAKQQGIPVGPGRGSGAGSLVAYALDITNVDPIKYNL
                     LFERFLNPERVSMPDFDIDFCFERRGEVINYVTEKYGTDRVAQIATFGTLKAKAVVKD
                     VARVLDISFEESNRIAKMIPDDLGMTLSKAFAQSPDLVELEKKGGVYAELFDAARRLE
                     GMARHISTHAAGVVIGREDLTRYVPLYRDARTGAISTQYSMELLEPCGLVKMDFLGLK
                     TLTLIEHAQELIRVKNPAFSIEEIDEEDEATFAMLGRGESSAIFQFESPGMQNVLKEA
                     KPGTIEDLVALNALYRPGPMRFIPQYIACKHGRQPITYADPELEEELKNTYGVIVYQE
                     QVMKVAQIIAGYSLGAADILRRIMSKKKVQELEAEKKKFVTGALALGRSKEHAIEIFE
                     MLEPFANYGFNKSHALAYSVIAYQTAYLKANHPAEFWAANLTNEMSNPDKFSEYLQTV
                     RAAEIEILPPSVNYSLRSFVVVGGKIIYGLAGIKHVGEGVVDAIVTERDANGPYTDLI
                     DFLTRLDSRVTNAKVMESLIKAGAFDELGTNRPTLLENLPDAVASVQRKKEFSAYGQI
                     SLFDEESEEMMDSFVMKEVEDWSTQEKLEIEKSLLGFYVSGHPLDRYRSAIKTRVTVD
                     TARMEHIPLGQQTNIIALLTQLKPHVTKRNDTIAFVQLTDLNTSFEGVIFADEYERVR
                     HFLQIDRIYGFEGTFDKRGNGDERISFRIDSVKTDPNELAPRAVSRCHVLLEKSFCTA
                     EQITRLRDAALDFGGSCTLYLYFKEQGRTLPSLVECGREFSVRCTADLAIELKKNSAV
                     LDVWFD"
     gene            complement(21916..22797)
                     /locus_tag="PLF67_05355"
     CDS             complement(21916..22797)
                     /locus_tag="PLF67_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOE89474.1"
                     /translation="MEATRSPMIHQPRTLNELSLLFQRIENPLLWAGGTHLMSKSDFY
                     PSSPNRQIIDLGEIEELKRISRTDRYVDIGSMVTTSSLLSAGKLVLPSILKSALGSIV
                     NHITRRQITIGGAVCEPESRMAFSTALAVLDAQAEIRRWEEGRFTSAWIPVNRLYDKN
                     GSLLLRDAPFVLTKIRLGLEWGDFQRFVLIDNPIRKPDTAILVAFQATRNQTALTKVR
                     FSASFPKKAFYISPELENQLSLLNLPIHPDRVNTISYEMVHDLMNRHESITEFQAERT
                     RRVFESFLHELNIQTLS"
     gene            complement(22784..23290)
                     /locus_tag="PLF67_05360"
     CDS             complement(22784..23290)
                     /locus_tag="PLF67_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOE89475.1"
                     /translation="MKLECTIDSKSLTLHVNSTKPLSLMLIEDVGVKSMLSHCQGNAC
                     GNCIVLVNDVAVLSCIVPAFRLKDAKIRTFETFHRTRLCRDIERAYRASGNKPCPDCY
                     ASKTLLIESILTELMSSTRSREEGMVDEKAIMQELSLNTCPCMDVTELLEITIQAFEY
                     RRRRRGSN"
     gene            complement(23287..25236)
                     /locus_tag="PLF67_05365"
     CDS             complement(23287..25236)
                     /locus_tag="PLF67_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOE89476.1"
                     /translation="MAEKRPTMGRLTPLQGVLITSRIARGRFDRIVVPEADPSFVIVA
                     PRDIPGSNRLRVLDAQIPLLSSGAITYRDQPLLALFGPNRESALVKAKGISVTYVEDE
                     PVDSLTVTPYSYTFGNLEEALKEENLNILERSYAFHSKTKKRLTYNRVSVMFDGETLH
                     IEAPTQWPAHVRESVAEVTGFSKKNVIIHRQPFYAPHDEMLILPAQLAALCSVAAIKS
                     GRDTEILYEMEAAPPRYEITRKTYYQSSGKPQAEEIRVVVDQGCASFFREELVNHLYT
                     GLIPLYPVTALTIHVEVHTTNNRPAHFYGGLGYSEALASSEAHYSELAKLTGYNPLSW
                     RMKFASENPSSGAMIRTDKYTKLKELASTVSKRADFTRKNAAYEMQRQMRIRHSTFFK
                     YGRGNGLALAPGIGGFSREYKGTPTQSVQVTLMPNDHVVINTSFYSIGNSAEIWKQIV
                     SDELGVSKKDISFVEDGNELLDSGPSVLNANSGRMPRHILRACQLIKEKRFVEPLPIS
                     ETVTFFRHPSAKDSLFFSNSWVAIILELEISPLALVPIIRSIWVSITTPKVYERAAYI
                     SKVRHTIVTTLLEAGAQLSQSRGFAIDIQLEEEGEGMPSSMTAAVKGAVTAALLTALE
                     QAVGSSVERIPVDGPTILNLLRSKH"
     gene            25321..>25456
                     /locus_tag="PLF67_05370"
     CDS             25321..>25456
                     /locus_tag="PLF67_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89477.1"
                     /translation="MDTIEREITKLRSELEAYQRAYYVDSRPLVDDSEYDRKFDRLLE
                     L"
BASE COUNT         5171 a         6910 c         7460 g         5915 t
ORIGIN      
        1 gcgcgggggt gtttctctgg acttctcgac gatctgatca caaggcaggt cttccccgca
       61 tgcgcggggg tgtttcttac cggcttgatg ccctgtgggt cgccggagag tcttccccgc
      121 atgcgcgggg gtgtttccga cctcgtcgaa ggcgtagggc gatccatcga gtcttccccg
      181 catgcgcggg ggtgtttcta tttgtttcgc cctacataat atgaaccacc agtcttcccc
      241 gcatgcgcgg gggtgtttcc ctcatcacgg aggagccggg cggggtgctg gagtcttccc
      301 cgcatgcgcg ggggtgtttc ctttataatt gttgggatat tttttctgat caggtcttcc
      361 ccgcatgcgc gggggtgttt ctgatagctt ctatgtggta agtgataatt tttcgtcttc
      421 cccgcatgcg cgggggtgtt tctgggcgag ctggtggagg actatgacca cgagggtctt
      481 ccccgcatgc gcgggggtgt ttcccgggcg accagagagg gtcatatcac cctcaggtct
      541 tccccgcatg cgcgggggtg tttctgaaga aaaagaaaac aaaaacgtat ttgcacagtc
      601 ttccccgcat gcgcgggggt gtttctcagt acggatggcg gtgcagccat ccgattacgt
      661 cttccccgca tgcgcggggg tgtttcccga ccaaaggcca gccaggagtc cggcaagagg
      721 tcttccccgc atgcgcgggg gtgtttcttg acggtcgccg ggcgggttgt ggagatcaag
      781 gtcttccccg catgcgcggg ggtgtttcta aatcgtgtac tctccaacca atcctagcat
      841 tgtcttcccc gcatgcgcgg gggtgtttcc cgaccaaagg ccagccagga gtccggcaag
      901 aggtcttccc cgcatgcgcg ggggtgtttt tgctctattg tttcttcctg ctcgggttca
      961 catgcaccat gcagtgcttg accgtgggga aggaggcttc gatcgtcttg tggacgcgtt
     1021 cggcgattga gtgggcttcg gtgagcgtct ggttcgggtc ggcggcgatc tccacatcga
     1081 catagcatct cgaaccgaat tgtcgtgttc gtaataaatc gactttcaag actccttcct
     1141 gtgcttcgat gagagctctg acctctttgg tgaaggactc gtcgcaggct cgatccacca
     1201 tctggttcat tccgtcccgg aaaatctcga caccgacccg gaagatgaag atggcgatga
     1261 ccaccgctgc gaccgcatcc ccgatcggat atcccatccg ggagaagagg ataccgatga
     1321 gtcctccgga ggtggcgaac acatcgctca gcgagtcgcc cgccgaagcc atcagagcgg
     1381 gggagccaac ctttttgccg gcccattgag tatatatata catcgctcct ttgatgatga
     1441 tcgaagccag tgaagcaatc actgtgatga atgtcggaac gggggtttcc agatatgtct
     1501 ttttgatgat cgacgagata ccgctgccga agagggcgat tccgacgaac atgatgatgc
     1561 cggacagcag aatcgccgcc acgcactcaa ggcgctcgtg cccgtattga tgatcatcat
     1621 ctgccttctt cgagccggcg gagatcccga ccatcgtgat gatcgaggaa agggtgtcac
     1681 ccagattgtt gataccgtcg ttcagcaggg cggcggagtg ggcgaagtat ccgatgagga
     1741 ttttagtgat ggctaaaaaa gcgttgctga ttagattgat ccgggcaacg gtcgttgcca
     1801 atgtggattt gctcatatgt ggaaagcccc cggatcgggg ggcttttcgg ttactccttc
     1861 ttctcagggt agacaatctt caggtgaagc tcttcaagct gcttctcttc cacgaaggaa
     1921 ggagaatcat ccatcggact gatacccgcg gtgtttttcg ggaacgcgat cacttcgtgg
     1981 atcgagcttt caccggccat gatcatggcg atccggtcga ttcccggggc gatccctccg
     2041 tggggcggag gactgaagct gaaggcggtg aggaggaagc cgaagcgctc ctgggcggtc
     2101 tcgtcatcaa aattgcagat gcggaagatc cgcttctgca gctcaacgtc atggatccgg
     2161 atcgatccgg aagccacttc atacccgttg agcacgaggt cgtacaaatc tcccttcacc
     2221 cgacccggat cctcttccat cgtatcgatg tactccgcct gcggcatgct gaacatgtgg
     2281 tgggcggctt cccattgatc gcgctcctcg ttgtactcga agagagggaa gtcgacgatc
     2341 cagcagaatt cgaagcccgg cctgatcagc tggaggtctt ttccgagctt ggagcggact
     2401 gcactgaggc ttgcacaggt ctttttccac tcattgtggg cgacaaagag gatcatgtcg
     2461 ccctctttcg catcgaagcg gtcgatgacc tccccttgaa tgccctcgaa gaacttggaa
     2521 acgccgccgc ttgcggtctt gtccgctccg actttcatcc acgcaaggcc gtgagcccca
     2581 tagactttcg cagcgttctc cagatcggtg atgtatttgc gggaaaaatc gacgatttcc
     2641 gaatgaggtg cgcacagcgc cttgacgtgg ccgccttggg cgacgatctc cttgaacgtg
     2701 gagaacgtcg atcgcgaagc gagatcattc atatcctgca gctccagacc gaagcgcaga
     2761 tccggcttgt cgcacccgta ggtgttcatc gcttcctcat aggagaaacg gcggaaggtg
     2821 gggggaagat cgtagtccat cacttccttg aagacatatc cgaacagccc ttccatcgtg
     2881 gagaggacat catcccgcgt cacgaagctc atctccagat ccagctgggt gaactccaac
     2941 tggcggtcgc cccgggcatc ctcgtcgcgg tagcagcggg cgatctggaa gtacttgtcc
     3001 attcctccga ccatcaggat ctgcttgaag agctgggggc tctggggcag ggcatagaac
     3061 ttgcccacat ggatccggga aggaacgagg aagtcacgag ccccctcggg ggtggactta
     3121 atcatcgtcg gagtctcgat ctcgtagaaa ccgcgggctg aaagatagcg gcggatcgcc
     3181 cagaccatat catggcgcag cttgatccgc ttcttcatcc cctcggtccg aagatcgagg
     3241 tatcgatact tcagccgcag ctcctcccgg ggaacgttgc cgtcatcgat ctggaaggga
     3301 agcggtgcgc aggttgaaag aatctcgatt ttttccgctt tcacttcgat ttcgccggtc
     3361 gccatctcgg gattcaccat cgaagcgggg cgggcccgaa ccacaccggt aacggcaatg
     3421 cagtcttcaa ggttcaatga aagggcagcc tgctgcagct cttttgatga atcgtcatcg
     3481 acgatgactt gggtgatccc gtaacggtcg cgaaggttga tgaaatgaat tatcccatgg
     3541 tcgcgatgcc ggtgtaccca gccgtttaga acaacggtct ttccgtcgtc ggctttggtc
     3601 agactgccgc atgtcgcggt acgctttgaa aaatgttcag ccatgtgaac ccccttcaag
     3661 taaacaaata ctatacgata gggggaagta tggcaattga ttgaaacact ctcatagtta
     3721 ccggggtgct tgggaccggc aatattgaca aagcgtatgc aagggatcaa tactggattt
     3781 gtaccggcga agtggttagg tccgcgaacc tttcaagtac ccggtttgat gaagagcgca
     3841 ctccgagggg gtgcgttttt cgtggcttca caaaaaacat ccccgcgggt gcggggaaga
     3901 gatcgaatga ccgggtttct atggacgaac ggaaatcgaa ccgctgaccg tttgaagggt
     3961 gatcctggaa gatccgtcgc ctttcgtcag ggtggagcgc tgttcgcctc caatcgtgat
     4021 cgagccgctg gtggtgtgcg cattgatggt gtagtggtcg gctccaagca gatccgcctc
     4081 cacctgtccg ctggtggagg agagggtgac atcggagggc gaaggcagcg ggagggccac
     4141 atccacgctt ccgcttaccg aattgacggt gattttgggc gcccgaaggg attccacctc
     4201 gattcttccg ctggtcgatg agacttcaat cggaccattg ctcgtaaggt cgccggcatc
     4261 caccgaaccg gaaaccgacg agagggaaat cgtcttgttc gcggtgatgt ccagaacgga
     4321 aaccttcgaa ctggtcgatg agatttctgc ggagttgagg tccaacgaat tgagaaccgt
     4381 cactttgccg ctgatcgaag cgagtgaaag atccttgatc cgatccgggg gagcggtaac
     4441 gaccagcgtc gacgaggggg agccgaagaa gacgggaaat atccatatcc gctttttggg
     4501 tgaaaccttg attgaaaggt tcgaaccgtt ctttgcaacg gcaagctgct tcttatcgat
     4561 cctcccgatc tcaagggaga ccgaggtcgc ttccggattg atctcgagga cgatcgggtt
     4621 ggaaaccgct gaaacggaaa gggaatcgcc tttggcgaag ggaatcgctt cagtgtcgcg
     4681 aatggttttg ccgcggaaga cctctccccg cacccagaca aagcctaggg cgaggaggat
     4741 taccaaggcg atcatgaaca atttgttggc tttggtgtct ttcaacatat cattttcccg
     4801 agaagaagcg atcgtcccaa tcacgctctt tgttgagtac ttcgctttcc atctcttcaa
     4861 cctttttctt caggttttca tacttgcttt tcagctcgct gtcggagacg gtctccccgt
     4921 gccgctgttc aaacgtccgt ccacccgggg ccatcgggat gatcagggcg gctccgaggt
     4981 aaatcagcgc tcccggaaag attccggtag tcaggattga aatacctacg atcaggcgaa
     5041 cgatatccac gttcaaatcg cgccactcag cgattcctgc acagattcca aagactttac
     5101 cattcgggga tcggtataat tttcttgtag ccatcgttct cattccttat tccttgttgt
     5161 aggagttctt tcgactccgt aagatttctt ccagattgtt gagacgctct tcaaggtcga
     5221 cgatcccttg ctccatctca acccgggtgc tgcctttgga ggcatactgt tcatacgttt
     5281 ctctgatcga tttttcagca tccttgttgg aaaacttctt cgaccacttc ggggaggagt
     5341 aggttcccgg ggggtagcca agctccattt cccgcatccg aagcgccgct tcgatcttcc
     5401 gattccgttc cttggatgaa ttctccaaga cattgagaat gatgacgaag gtgaagatca
     5461 ccgcgaggat cggtactgcc gtcatttctt gcctcctgcc aatttgcgga gctcgtcgag
     5521 ctctgcttca agctcttcca tctcttcaag cttccggaac tgctcatcga gggaagcgtt
     5581 ccttcgattg agttcattgc ttgcctgcat ccggtcgatc tgatcctgca tctcggcgaa
     5641 ccggtcgtcg gtgcttctcc tcatcgtcgt gttgacgact tcctcttcct tcgcccgatt
     5701 tgcccggtcg accatcgtct ggtacttctg cttgacggaa gcaagcttct gctcaatctg
     5761 gtcgatctcg ctcttggcga cgctgatcag ctcttcgctg ctcgcgatct ggctgagaag
     5821 cggctcgagg cgctcggata cctggcgttt tgcgagcagt gcctcccggg caagatcttc
     5881 ccggcctttg ctgatggcca gttccgcccg agcctgccag cgcttcatct cttcctcgaa
     5941 ctcgttgtac tcccgcttgc tttttgcaag ggtcgccatc tttgcggcgg cggaggattt
     6001 caactcgatg agggtgtctt ccatctcctg aatcataaga cggatcatct tttcaggatc
     6061 ttccgcctta tcaagcagtg cgttgatgtt ggcatttaca atatctaaaa atcttgaaaa
     6121 tactcccatg attacgctcc ttctttagtt ttatagtaat tggtatgcat gaagcgtgcc
     6181 aaccctgaaa ccgtcctttt tgagcgtgta cttccgattt ttatctccct gtgctatggt
     6241 aattgtggtt ttatatgcca aggatggtaa attacaccaa aggaacaggg tcatggcggc
     6301 tcaagaacga tattcccaac aaccccttgg agaaagcgag gtgtttctcg attttcaggc
     6361 gaagctcagc cgcgtagctg cgataaaccg cccggttctg cttgtcggcg aacggggaag
     6421 cggaaaggag atcgccgctc gaaggctcca ctacctctcc ccccgctggg acaagaacct
     6481 cgttacggtg aactgcgccg cccttcctcc ctccctgatc gaaacggaac tgttcggcta
     6541 cgaacaggga gcgttcaccg gagcccagaa aacccgtaaa ggccgctttg aagaagcgga
     6601 agggggaacc ctcttcctcg atgagatcgg cctcatccct ctggaggttc aggagaagat
     6661 cctccgggtc gttgaatatg gaacatatga acgggtaggc tcctccgtta cccacgaagt
     6721 gaacgtccga atcatcgggg cgacgaacgc ggatcttccc gccctgtgcg aagaggggaa
     6781 gttcaagcag gacctcttgg accgtctttc cttcgaagtc ctctttctgc cccccttgcg
     6841 ggagcggggt gaggacatcc tcacccttgc ctacttcttc gcttcgaaga tggcgcagga
     6901 gtgcgaccgg gaagggatgg tcaccttcac cgacgaggtg atcgacaagc tcatggcgta
     6961 ccggtggccg ggaaacgtcc gggaactgaa gaacgtgatc gaacgggcgg tctaccgcag
     7021 cgaggacaac gtcatcaccg aacttcagtt cgatcccttc gccaacccct tcgaggaaaa
     7081 aaccggacag tcgaaagagg ctgaacttgt cttggggaac tttgaagcgg atcggatcga
     7141 gttcgagatc cgctaccttg ccgaagcgct caagcagggg gaagggaacc agaaggaagc
     7201 ggcgaagctc cttgggctca cctacgatca gtttcgggga ctgtatcgaa agtatcagga
     7261 tcgcctatag aaagagaaac gggcatcgcg tgatgcccgt ccatggagaa atacgtctga
     7321 cgcttactcg gttttcgccg gagtcttctt tgccgagctc ttcttcagtt cggcaagctc
     7381 cgcttcgatc gcttcgtcgg tctccatctg agtgaactgc tcaaggaccg aggtggaacc
     7441 gctgtaggcg gccatttccg cttccgcttc cattcgttcg atcttcgcct caagttcgct
     7501 gaacttcgac gcaatgttcg atgagtcgct ttcccttagg accgcagcca cctgacgacg
     7561 ctcccgggcg ctgatcgagc gctggacgag gatctgctgc ttgtccttca cttccttcag
     7621 cttgtcgccg atctgggcaa gctgggtggt ctgggcggcg atgatccctt ccagattggt
     7681 gatctgctcg gctagctgat cgtgatgctc ctgagctttc cgcttctcca ccagagcctc
     7741 gcgggcaagg tcttccttcc cgttcgccac cgcaagcttg gcgcgctccg cccagcgatt
     7801 caccgcatcc tgtgcttcct tcacccggtg gttcagcgcg ataagctccg ctttctttcc
     7861 cgcgatcgag gagcgggtct tcgtctgaag ctcctccaga ttggtgacca tcagggcgat
     7921 catcttctgc ggatcctcaa acttatctaa agcgctgttc acgttggagc tgacgatatc
     7981 tctgactcgt ttaaaaaatt gcattgcatg cctcctgttc ttctttggta tctttcctaa
     8041 tgcagaaggc gtgccaactc gtgaaaaaga gcaataaatg agtaattcag aggtgtattt
     8101 ttctataagc ccaccttttt ggttaaatat accaatgccc gtggttataa ataccaccat
     8161 atgatcatca tgggttgtct tgttccctca gccccggagg tgtagactga aacaatgagc
     8221 accctctata tcgtggcgac cccgatcgga aacttggccg acatgaccta ccgagccgtc
     8281 gaagtgttga cgaacgtcga catcatcgcc tgtgaagaca cccggcatac gcagaacctg
     8341 ctgaaccact acaagatctc caagcgcctg atcgcgaccc acgcccacaa tgaagacaac
     8401 tccgccaaag ggatcatcgg cctgcttgaa gagggcaagg atgtcgcctt tgtctccgat
     8461 gccgggactc ccggcatcag cgatcccggc agccgcctcg ttgaacgcgt ccgctcatcc
     8521 ggctttgacg tggtgccgat ccccggcgcc agcgccttca ccaccttggt gagcgtggcc
     8581 ggttacatcg gtcgaagcat ccacttcgag gggtttcttt ctcccaaaag cggaagacgg
     8641 aagaaacgtc tggaagagct gcttcagcgg gatgaaagct tcgtgttcta tgaatccccc
     8701 tttcgagtgg tgaaggttct tgaggagctt tccaccttgg agcccggccg ccgggtcgtg
     8761 gcggggcggg agatgaccaa gaagtttgaa gagtttctga ccgggtcggt cgaagaggtg
     8821 cttgattcac tgaaaggacg acaaaccgtg aagggggagt tcgcccttct cgtggcgccg
     8881 aaaggcgtgg gagcgagcga tgattacgat caggaaactg aagagcctta aggaagacac
     8941 ccgcctgcgc aaagcgacgc ttcttttaaa ggaactcagc cggctcggtg agattgattc
     9001 ctcatatgtg aaggaccttc ttcagatcat caaagagtca agggcgggtg gggataaacg
     9061 ggttgttggg cttatagatc gcattggcgg gcaggaggga agagccagag cgttttcctt
     9121 ggaggacctg cactaccggc tccttgactt gcttggagga cagaccgccg attgggattt
     9181 cgtcgacgaa gagacgagcc tcgacatcgg gcaacgagtg gtttgcgagc ggtatctggt
     9241 ggtcgatcgg atccgctcgc cgttcaacct cggttcgatc ttccgcctcg ccgactcctt
     9301 cggcatcaaa aagatctaca tcgtggaggg tggggcggag ccgacccatc agcgaacgat
     9361 aaaggtcggc agagggacgg tggaaaccgt cgaatatgag gttgtttcgg aagactcgct
     9421 gcttgcaggc ttgaagaaca gcgagctgcc tctctttgcg ctggagagcg ggggagttga
     9481 tatcaccgag ttcgattttc cccttgcagg aatctgcgtc atcggcagcg aagagctcgg
     9541 agtcagtccc gctctccttg atctgtgcga caagcgttcg gggcgggttt cgatcgccct
     9601 gggcgggacg aagggatcct tgaacgtgac ttcggcgagc gcgatcatgc tgcacggctg
     9661 gtttgttcgg taggatcagt ttcttcccga caggcccgcc tggtacatcc gcttcaagcg
     9721 gttctcgata tcgtagttga agtttcccgc gctccagttg cctttggcgt ttctgacccc
     9781 gctcggccgg ccggtaagaa tccgcatccc ctcttcgatc gttctgatcg ggtagatgaa
     9841 gaagatccga tccctgatcg cctgaaggat ctcgtaggag agaatgaggt tcgagacgtt
     9901 ctggtagggg atgatcacgc cctgttttcc ggtgagaccc agaaagcggc aggtgtggaa
     9961 gaacccctcg atcttttcgt tgatcccccc caccggctgg atctccccca tctggttgac
    10021 cgagccggtg acggcgatgt cctgacggac ttcaagctcc ccgatcgccg aaagcagggc
    10081 gaagagctcg ctggaggaag cgctgtcccc atcgacctcc gcataggact gttcgaaggc
    10141 gatcccggcg tagatcgaaa gagggaagga gcgggcgtag tgcttgcgca gatatccctc
    10201 caggatcaga agacccttgt cgtgaatctc gccgctgagg cccgcttcgt gctcgatgtt
    10261 gacgattccc tccgttcccg gggcgacggt ggcgctgatc accgtcggcg ttccgaagga
    10321 gtaggcgccc tggtcgatga ccgcaaggcc gttcaccacg ccgactttgg ctccctggag
    10381 gctgatgatc acctcgccgt ttttgatctg ttcgttgatc ttctcctggt agatgccgct
    10441 tgcataggag cgctcctcgt tgaccttgag gatcatcgag cggtcgatca tttccttccg
    10501 ctcctgtttc gcccagtaat tagcctcgtc caagagatcg tagagaatcg gaaactgggt
    10561 ggaaaggtga aggcgcgatt cgacaaacca tgcgctgtag cggagcattt gggccagccc
    10621 gtcatcggtg atcggaagga gccccttctc cgaaacgacg tgctcgatgt ttgcgatcgt
    10681 ctgaccgatc gtctcctcgg tcaggttcat cgtgtaatcg aactgggcgt tgaccttgaa
    10741 gagggaaaga aaacggtcat ctttctcgct gagagcgtcg tagagctcct cgctgccgat
    10801 gagaatgacc ttgatcgaaa ggggcggaat ctgggggcgg atgatcgaat tcgccaattc
    10861 ccctttcact acgttccctt ctttctgtgc aaggttcgtg gcatcgagat accgcttcaa
    10921 cgcctcccaa agcccttcct tgctgaagag ctcggccgca tcgatgatga tgaaccctcc
    10981 gcagctttcc aggagcgaac cggggtggta ggaaaggtgg gcgggcttgt cctttgcatg
    11041 gatcgttccg aagaggtttc ccatcgaagg atggctttca atgatcatcg gccgcttcgt
    11101 cgagcggctg tggtcgacga cgaggttgcc cagatagcgg gtggccagcg cttcatccgc
    11161 tttccccagc cgccggatgt gggcatcctg gcgggtgatg tcccctttca gaccgaagaa
    11221 atagcgttca agctcctttt gggggaattg ggcgatcagg gaatcgacga gggtgagggc
    11281 gttgctcaag aacgtttcgc tctcctcgga aaggagggtg atcgagtggt tgaactgctc
    11341 aagcgaatcg atgaacctcg agctctcgcc gggggcgaac gtgagcacgt gcgggctgtc
    11401 cggttgggtg aagttgcaga catacacgat gtccctgagg ccggagaggt cgtcctgaac
    11461 ctgttcgatc gcatgacgga cggcggcgtg gcggccgctc tggctctccc cgctgacgaa
    11521 gatgttgtat cccgctttcg gcagcgaaag acccatctcc aaggaacgca aagcccttgg
    11581 ctgaccgacg atccggtcgg ccaaccgttg attccgcaac cgcttgatga gggcgggatc
    11641 gaactcgaag cttgcctgtt caaaggaaag cggcaatact tgagaggtgc gctcgctcat
    11701 gagcgaacta taggaggaga gaccgatggt gtcaactgat ggaatgatgc gaagcgaact
    11761 ggtcgcctat ctggatgagt acctggggat cgagagcttt ctctcatcgg acagctcgct
    11821 caacgggctg gtggttggcg gcgaagataa acggatcggg aagatcgggt atgcggtgga
    11881 tgcctgccag gcctcttttg atcaggcggc agaggagaag gtggatttcc tgctcgtcca
    11941 ccacggtctg ttctggggag ttcccctcgc ggtaaccggt tcccactata cgagattggc
    12001 gaccctcatc aaacacggca tcgatttgta tgcggcgcat ttgccgttgg atgctcatcc
    12061 ggaaatcgga aacaacatcc ggatcgccaa agcgctcgac ttggaagaga tcgaaccgtt
    12121 cgctccctac aaagggaaga cgatcggcta taaagggcgg ctgcaagagc ccaagacgct
    12181 gaaggagatc gccgggcatc tcggatatga gaaaacgatc cttctgccct tcgggaagga
    12241 gaggatccga acggtcggaa tcgtcagcgg aggggcgagc cgaaacgtct ccgatgcact
    12301 gaatgatggc ttggatctct atataaccgg cgaggtggag catgaaagct accatgtggc
    12361 gctggaaggg ggaatcaatg tcctcggcgg cggacattac gttacggaag tgtacggagt
    12421 gagggcgttg gccgaacacc tgtgtgagcg cttcggcctc gaagcggtgt ttattgccaa
    12481 cccaaccggc ttgtagtata gtttgccgta tggcaaaaac gtggcgtcct ctcattctca
    12541 ccgtgttcat cgtgcttgcg gatcagcttt ccaaagcatg ggtcgtcgcc gcgattccgg
    12601 agaataccgt cggctaccgg ttcttcgggg attttttggc gatcgtccac gtccgaaaca
    12661 ccgcgatcgc cttcagcatg ggttctgcgc ttcccatttg gatccgactg atctttttcg
    12721 taggtctgcc ggtagtgttg ctggtgatcg ttctgctgat ttacttcaat gagcatattc
    12781 ccctctcgat gccgacccga tggacgcttg ccctcttcat gggcggtgga atcggaaacc
    12841 tcattgaccg gatcttccgc tccctcaggg tcgtggattt catcagcgtg aagttttacg
    12901 ggatcttcgg cctggaacgg tggccgacct tcaacatagc cgatgcgtcc ctggttgtca
    12961 gcggcatcct tctcgcagta ttattacttt tggagaagga ggtggacaga gatgggaaag
    13021 aaaggtaata ccgcattgtt catgatcatc gcgaccttgg tgaacatcct gttgatgatg
    13081 atcctgttca tcatcggttt ttttctcctc gtccgatttg tcgaccccga gtcctcgatg
    13141 gtgccgatct atcttgcctt ggtcttcttc tttgcgatcg gaggatcctt cttcctctat
    13201 tcaaagcttg tcacctgggc ttcgaaacgc tttaacctgg aagataagat cgttcccctc
    13261 ttcgggaacc gaagacgaaa accgcccaag cgagattgac gatgcccctt tcgattgctg
    13321 cgtacgccaa gctttcgtgg tacccattca gcgatgagcc ggtcatcaac agcagttggt
    13381 acatgaaccg gctcggcgat ccgtcgattc tgctgcccgg tgaatcgccg gacaagaagt
    13441 ggcacctctt cgggcataca tttcttggaa tcgttcattt catcagtgaa aacggaatca
    13501 gctgggagcc gcgcaagatg atcgaactgc ggggacactc tccatccctc tacatggagg
    13561 atgggatgta ctacctgatt tacgagaaac atgatgcgat cccttcgcag atcgattcga
    13621 tcttcgcaag gcggaaacgg aaagatcggg tcagccacag tcgcatcgaa atgcgttcct
    13681 ccgccgatct gatcctgttc agcgaaccga agatcatcct cgacagtcgg gagatcccct
    13741 tcgccgccga cggcctgggc cggccccggg tctcccgccc tcagctgttc aaggatggag
    13801 gagtctacac ccttttcttc ggcgcctccc acatcgtgct tgaggacagc aggcataaat
    13861 cgagccgcta cttcgcttcg gcgacctctt cagacctcct tggtccgtac cggttggcca
    13921 accatggcag gcccttgatc gaaccggacg gagatgaccc gaaccgatcg cttgccgtcg
    13981 gttccgtccg ggtcctgaag gcgagcgacg ggtacgtcgc cctccaatgc gggaagtatt
    14041 gggataggga gttgaagaaa agctccacca gccttattca acttgaatcc gtcgacgggg
    14101 tgaattggct cccttcaaca cggccccgga tcctgagtcc tccttcagag gggtgggcga
    14161 gccgttatat catttcatgc gatgcccgct ataaagagga tgaaggatgc gtgtactgct
    14221 acttcagcgc aagctccaag agaggatttc gtccggtcaa agaggagatc ggcctgctct
    14281 tgggaaagga tcccgtcctc agaaaagtct tcctctagaa ctcgcttcgc ttcttccatt
    14341 cggtgtgggc ggtgaagacg atctcttcgg cgccgtcggc cgtctttcgg acgatcgtgc
    14401 tgaccagaga gggcgtttct tcgttgtatc cctcttcatc ggctgcatag gtatggacga
    14461 agagcagatc gtcaaggaag gtctgcttct ggtagagcac atccgcgctc ctgaccttgt
    14521 aggttgttcg gaacttcgcc ggcagcgcct caagaaccca cgagaggtag gagacgttgt
    14581 tgatgtgctg attccggtcg gtatcaagat aggtgacccg cggcgcatag cttcccagtt
    14641 gatgaagggt ttcaaaatcg acggttgact tcgggagatt catatcgatg gctcgggagg
    14701 aatctttcag caatccgatc cgttcgttca tctcctgagg ccgaagcggg cgggagttct
    14761 tcaggtcgat gaccgcccag aaggtcttcg cctgaaagag cggagaaccg tcggaggcat
    14821 agccttcgac gatccggggc aggtgaagcc ggatcggctc ctgggcccag gtgttcacat
    14881 gaatctgttc attccaccgg gtgtagccga agatctcgat tcggttccgg gtgatcaccc
    14941 acgtcttgcc ctcggcgtgc agatccatga tcgagcagcc tctcatcgcc gcgtgcaaac
    15001 cggccgcttc ctggacgatg cggaggtaga acgccgtctg ggctgccccc aggctgtcgg
    15061 tttcaaagct gtacgtggta aatcgcaagc ttgcgatgtt ttcggcgctg attgcgaccg
    15121 gatcattgtg attcatcttt tttttccttt tatagaaccg cagggtgctg cggccgtatg
    15181 agcgttcatc gatgaagaga agatcgccca gctgctccgg ccactgtgct ttctcttcga
    15241 ccgggtagtg gatgataaac aatccttccg ctttgaggat cttgtttcgt tcaactgctt
    15301 caaggaggcg gatcttctct tccataggaa aggggggatc ggcatagacg acatcatatg
    15361 cgatcttcgc ctgcgtaaga aatcggaaga catcgcttat gaagagcttg atcggggtct
    15421 cgacgaaggc gatgttcttc atgatcgtct ctttcttgcc ccggtcccgc tccaccagat
    15481 ggacaagacc ggctccgcgg ctggcggctt cgatgccgat ggatccgctt ccgctgaaga
    15541 gatcaagcca ggccgttccc tcctggtttc cgaggattga aaacattgac tcgcgcatcc
    15601 ggtccatagc cggacgaatg acgccgggcg ggcagacgat cttccttcct cggtagaggc
    15661 ctcctgttac acgcatgggt tcatcctagt ggttttcgaa agtttgctcc agaggactct
    15721 acgcttcgct ccgcccggct tcaaacggtg gagcttcact gaggactgag cggatgatcg
    15781 tattttcggg ggcaaggaag ccgctgtcga cttgaaggat ccggtccgag gccgccctgg
    15841 cctgttcgat cagctccaga tcatccgaga gcgaggcgaa ggccagattc aggtagccgc
    15901 tttgagcccg accggtcaat tcgccgggac cgcggatgat gagatcctgt tcggcgatat
    15961 ggaacccgtc ggtggaatcc ttcatcacct gaaggcgctt cttcgcctct tcggtcagtt
    16021 cgtcggagaa gacgaggaaa caataggatt gcagggcgga gcgcccgacc cgcccgcgca
    16081 gctggtgcag ggcggcaagt ccgaagcgat cggcatgctc gatgatcatg caggtggcgt
    16141 tgggaatgtc gatgccgacc tccacgacgc tggtggagac aagataggag agctcgcctt
    16201 tccggtacgc ttcgagaatc gccaccttct cctcctcatc cagcttggag tggatcaacg
    16261 ccgagggaac gccgggatac tgctgcttga ggaactcgta catgcccgcc acgtcccgaa
    16321 ggtcgctctg tccgctgtca tcgatccggg gatagatgaa gtacgcctga tgaccccggg
    16381 tgaactcgac cccgaccgac ttgtacatcc ggattctgct ctcttcccgt accagaaggg
    16441 tgatgaccgg cttgcgcccc ggcggcatcg tctgaagcgt cgaaatgttc agcgacccga
    16501 agacggtgag ggaaagggtc cggggaatcg gggtggcggt catcagcagg aggtgcggct
    16561 ccgtcgcctt gccgaggaga gcgaggcgct gctccacccc gaagcggtgc tgctcgtcga
    16621 tgatcacata ccggaggttc ctgaatgtca cgctctcgct gaagagggca tgggttccca
    16681 ccaacaggtc gatctctccc cgttgcaagg cggtcagcag ataagagcgc tctttcggtt
    16741 tgaccgagcc ggtgagaaac gccagacgga ctcccaaggg ttccaacagc gccgccccgc
    16801 tctcggcgtg ctgccgggcc aagagctcgg tgggggccat gaacgccacc tgaccccctt
    16861 cggcgatgat cttcagggcg ctgatccatc cgaccagagt ttttcccgag cccacatccc
    16921 cctgaagcag acggttcatc ggctcctccg caccgaggtc ttcgcggatc tctttcagcg
    16981 cggccagctg atcgttcgtc aaggcgaagg gaaggcgctc gatgaggcga agctccaagt
    17041 caccgggaat cgccggcttg gccggtttct ttctcggctt tcgtcgggct acgagctgaa
    17101 ggtagaacaa ttcggtgaag gcgagcgttc ttcgggcccg ggcaagttgc tgaagattcg
    17161 gggggaagtg gtaggtgcgg atcgcctcgt cggtcgatag aagatccagc tccttcatca
    17221 gcgactcggg cagttcattg atgaacgctc cgcaccgggt gagaacctgc cggatgttcg
    17281 cccggatgag gcgctgggta agggaaccgc gcagcggata gacgggaaga atcttcctga
    17341 actgcggggg aaaatccccg tcctcggtgg cggggtgaag ttcgaattga acggcctgct
    17401 tctccgaacc cttgatcgca accggagccc aaagataata gatccttccc ggtttgatcg
    17461 tcttttccag aaagttccgg ccgaagcaga ggagcgaaag gcgcccgtcg ccaaggccgc
    17521 tgacatcgcg gacgatgatc ttgagggtcc gtttcatccc ttttttcaac ccgaaatagg
    17581 agtggctgag cacctccacg agggtgttgg cgacctggcc gtcctgcacg ctgcccaatg
    17641 gacgcaccgt tgaacggtct tcccacgttc gcggggcgag gagcaacagc tcgctctggg
    17701 tgtggatgcc cagctccgca tatgatctgg tcgccgccgg acccacgccg ccgagcttgg
    17761 caaccggatc ggtgatcgtt cggagatagg tcatcgtcag attcctgttt tagtgagcaa
    17821 cgaaagcagg aggcggagca gaagggagga accgatccat gcgatgatca ggacgaccag
    17881 gcttgagatc accaacttcc ggtcgttcac cgtttgacgg gcatagaagg ttcgatgggt
    17941 ccaatccaaa agccccccga tcagggagtc gagcagcgcg atataggtga ttgagtggat
    18001 cgagcgttta taacagaagt acagtcgaac gacgaacagg atgaaaaaga agaagaggat
    18061 cggatggacg atgtaggagt agagggtctg aagcagcagg gcgatcacca tcgtgacggt
    18121 gatggtcccg tagcttccaa agagccttaa gagggattgg atcactgaaa ggactgcaag
    18181 ggctacaagc ggagtcagat cgaaccgggg gcggacaaga ctgcgaatcc ccttgaacca
    18241 tgagaggtac ggatccacga tctttccgag atagtaggct agaggattct ccgtaccggt
    18301 cttcagccat gaaaggatga ttcgaatcca gatgagaagc gaatacagcg acaggaggct
    18361 ggctaaaaga tttgctattg tcatcaagac cgtgggggtt tgctgcatat cgatcgaaca
    18421 ctccttggct ctatcatact cttagtcgaa ccaaacgtcc aagactgcgg aattcttttt
    18481 cagctcaatg gccaaatcgg ccgtgcaccg gactgaaaac tcccgtccgc attcgacaag
    18541 ggaggggagc gttcggccct gttccttgaa gtaaagatac agggtgcagg aaccgccgaa
    18601 gtcgagcgcc gcatccctca gacgagtgat ctgctcggcg gtgcagaagg atttctccaa
    18661 caggacgtgg cagcggctga ccgcccgggg agccagctcg ttgggatcgg ttttgacgct
    18721 gtcgattcgg aaggagattc gttcatctcc attgccccgc ttgtcaaagg ttccttcaaa
    18781 gccgtagatt cggtcgattt ggaggaagtg gcggacccgt tcatactcat cggcgaagat
    18841 caccccttcg aaggaggtgt tcagatcggt aagctggacg aacgcgatcg tgtcgttccg
    18901 ctttgtaaca tgcggtttga gctgggtgag cagcgcgatg atgttggtct gttggcccaa
    18961 ggggatgtgc tccatccggg cggtgtcgac cgtcacccgg gttttgatcg cgctgcgata
    19021 gcgatcgaga gggtgtccgc ttacatagaa gccgaggagg gatttttcaa tttccagctt
    19081 ttcctgagtc gaccaatcct ccacctcctt cattacgaac gagtccatca tctcctcgct
    19141 ctcttcatcg aagagggaga tttgcccata tgctgaaaac tctttctttc gttggacgga
    19201 ggcgaccgca tcgggaaggt tctccagcaa tgtgggacgg ttggtcccca gctcatcaaa
    19261 cgcccccgct ttgatcaggg actccatcac cttggcgttt gtcactctgg agtcgaggcg
    19321 ggtcaggaaa tcaatcaagt ctgtgtacgg accgttggcg tcgcgttcgg tgacgatcgc
    19381 gtccacaacc ccctcgccga cgtgcttgat gcccgcaagg ccatagatga tctttccgcc
    19441 cacgacgacg aacgagcgca gcgaatagtt cacgctggga gggagaatct cgatctccgc
    19501 cgcccggacc gtttggaggt actcgctgaa tttgtcgggg ttgctcatct cgttggtgag
    19561 gttcgccgcc cagaactcgg cgggatggtt cgccttcagg tatgcggtct gataggcgat
    19621 cacggagtag gcgagggcat ggctcttgtt gaaaccgtag ttggcgaacg gctcaagcat
    19681 ctcgaagatt tcgatcgcat gctccttgct cctgcccaag gcgagcgctc ctgtgacgaa
    19741 cttctttttt tccgcttcca gttcctggac tttcttttta ctcatgatcc gcctcaggat
    19801 atccgcggct cccagggagt agccggcgat gatctgggcg actttcatca cctgctcctg
    19861 ataaacgatc accccgtagg tgtttttcag ctcttcttcc agctcgggat cggcatacgt
    19921 gatcggctga cggccgtgct tgcaggcgat gtactgggga atgaatctca tcggtccggg
    19981 acgatagagg gcgttgaggg cgacgagatc ttcgatcgtg ccgggcttcg cctccttgag
    20041 gacgttctgc attccggggc tttcaaactg gaagatagcg ctggactctc cgcggccgag
    20101 catcgcgaaa gtcgcctcat cctcttcatc gatctcttcg atcgagaagg cggggttttt
    20161 gacccgaatc agttcctggg catgctcgat caatgtgagg gtcttcagcc ccaaaaagtc
    20221 catcttcacc agaccgcacg gttccagcag ctccatcgag tattgcgtgg agatcgcgcc
    20281 ggtccgggca tcgcggtaca ggggaacgta ccgggtgaga tcctcccttc cgatcaccac
    20341 tccggcggca tgggtcgaga tgtgcctggc catcccttca agtcgccgag ccgcatcgaa
    20401 gagctcggcg tatacgcccc ccttcttttc cagttccacc agatccggcg actgtgcgaa
    20461 cgctttgctc agcgtcatcc caaggtcgtc ggggatcatc ttcgctatcc tgttggactc
    20521 ctcgaagctg atgtcgagaa cccgggcgac atccttcacc accgctttgg ccttgagggt
    20581 tccgaaggtg gcgatctggg cgacgcggtc ggttccatat ttttccgtga cgtagttgat
    20641 cacctctcct cttcgttcga agcagaagtc gatgtcgaag tccggcatac tcacccgctc
    20701 ggggttgagg aagcgctcga agagcaggtt gtatttgatc ggatcgacgt tggtgatatc
    20761 aagagcgtac gctaccaaag aacccgcccc cgaaccgcgc cccggtccga cggggatccc
    20821 ctgttgcttc gcccagcgga tgtaatccat cacgatgagg aaatatcctt cgaagcccat
    20881 cgagatgatg acctccagtt cgtactcgag gcgctcggtc aacttttcgg tgaccacttc
    20941 aaagcggttg gcgagtcctt cgcgggaaag gctcctgagg tattcggcag gggattcgta
    21001 ccccgaaggc acctcaaaga ccggcagctg gggaccgggg aattggatct ccagattgca
    21061 ccgttgggct atttcgacgg agttctcgat cgcctcggga acccacgaaa agagactcgc
    21121 catctcctct ttgcttttaa ggtagaactg gtcgttggga aagcgcatcc ggttcggatc
    21181 gtgcttcttc gcattggttc ccacgcagag gagcagatca tgggcgttcg catccgattg
    21241 ctcgatatag tggatgtcgt tggtggcgac gaggggaatg cccgtttcat cggagagccg
    21301 cttcaaaagg ggattggttc gtttctgctc ctccaagccg tggtcctgga gctcaagata
    21361 gtagcgcccg tcgtcaaaga cggagttgaa ccagagcgcc cggcgtttcg cttcctcata
    21421 gttgttgttc aagagatgtt ggagaatctc gccgccgagg cacgccgaaa ggcagatgag
    21481 cccttcatta tatcgttcca ggagatcatc atcgattcga ggtcggaagt agaacccttc
    21541 agtgtatgaa agggaggaga gaatcatcag gttctgatat ccgatctggt tcatcgcaag
    21601 gaggacgagg tggtattgct ggctcttctt tccgctgccc ggttcgggcc gttcggtccg
    21661 gtcgcttgga ttgacgtaga attcacagcc gatgatcggc ttgatcccca ccttttttgc
    21721 agcatagtag aaatggaggg caccgaacag gttcccatga tcggtgatgg cgatgctgtc
    21781 catcccgagg gcttgggctt ttttcatgta ggcggaaatc tttgccgctc cgtcaagcag
    21841 cgaaaagtct gtatggttgt gcaggtggac gaagccaccg ctgaaactcc gggtcatcat
    21901 tcctccaata tattcctatg agagcgtttg gatgttgagt tcatgcagga aggactcaaa
    21961 gaccctcctg gtccgctccg cttgaaattc ggtaatcgac tcatggcgat tcatcagatc
    22021 atgaaccatc tcgtatgaga tggtgttcac ccggtcgggg tggatcggca ggttcaacag
    22081 tgaaagctga ttctccaatt cgggggagat gtagaacgcc ttcttgggaa aggatgcgct
    22141 gaaacgaact ttcgttaagg ccgtctggtt tctggtggct tgaaacgcca caagaatcgc
    22201 cgtgtcgggt tttcgaatcg ggttgtcgat cagcacgaag cgctggaaat ccccccattc
    22261 cagcccgaga cggatttttg tcaggacgaa gggcgcatca cggagcagaa gcgatccgtt
    22321 cttgtcgtag aggcggttga ccggaatcca tgcggaagtg aaccgcccct cctcccacct
    22381 gcggatctcc gcctgggcat cgaggacggc aagtgcggtg gaaaacgcca tccggctctc
    22441 cggttcacag actgcgccgc cgatcgtgat ttggcgccgg gtgatgtgat tgacgatgct
    22501 gccgagcgcg cttttcagaa ttgagggaag gaccagtttt ccggcggaga gcagactgct
    22561 ggtagttacc atcgagccga tatcgacata tcggtcggtt cggctgattc gcttcagttc
    22621 ttcaatctct cccagatcga tgatctggcg attgggtgag gaagggtaga agtcgctctt
    22681 gctcatcagg tgggtgccgc ccgcccaaag aagggggttc tcgatccgtt ggaaaagaag
    22741 ggagagctca ttcagggttc gcggctgatg aatcatcggg gatctagttg cttccacgcc
    22801 ttctcctcct gtactcgaat gcctgaatcg taatctccag caactccgtc acgtccatgc
    22861 acgggcaggt gttcaggctg agctcctgca tgatcgcttt ctcatccacc atcccctcct
    22921 cccggctgcg ggtgctgctc atcaattcgg tgaggatcga ttcgatgagc aatgtctttg
    22981 atgcatagca atcgggacag ggtttgtttc cgcttgcccg gtaggcccgt tcaatgtccc
    23041 ggcagaggcg ggtgcgatga aatgtctcga aggttcgaat cttcgcatcc ttcaggcgga
    23101 acgcgggaac gatgcagctt aagaccgcca catcgttcac cagaacgatg cagttgccgc
    23161 acgcgttgcc ctgacagtgg ctgagcatcg atttgacccc cacatcttca atgagcatca
    23221 acgaaagcgg cttggtggaa ttgacgtgca atgtcaatga cttggagtcg atggtgcatt
    23281 ccagtttcaa tgtttactcc ttagaaggtt caaaatcgtc ggtccgtcca ccggaatccg
    23341 ttcgacgctt gatccgaccg cttgctccaa ggcggtgagc agggcggcgg tcaccgcccc
    23401 tttgacggcg gcggtcatcg aggagggcat gccttctcct tcctcctcaa gttggatatc
    23461 gatcgcaaac cccctcgact gggagagttg agctcccgct tcaagcagcg tcgttacgat
    23521 cgtgtggcga accttgctga tataggcggc tctttcgtac accttcgggg tggtgatgga
    23581 gacccagatg gagcggatga tcggcaccag agcgaggggt gagatttcaa gctcaagaat
    23641 gatggcgacc cacgagttcg agaagaagag ggaatccttt gccgaaggat ggcggaagaa
    23701 ggtaacggtc tcgctgatcg gaagcggctc gacgaaccgt ttttccttga tgagctggca
    23761 cgctcgcagg atgtgtcgag gcattcttcc gctgttggcg ttgaggaccg aaggaccgct
    23821 gtcgagcaat tcgttgccgt cttcgacgaa ggagatgtct ttctttgata cacccagctc
    23881 atcgctgacg atctgtttcc agatctcggc ggaatttccg atgctgtaga atgaggtgtt
    23941 gatgacgaca tggtcgttcg gcatgagggt cacttgaacg ctttgcgtcg gcgtcccctt
    24001 gtactcccgg ctgaacccgc cgattcccgg agcaagtgca agaccgtttc cccggccgta
    24061 cttgaagaac gtggagtggc ggattcgcat ctgccgctgc atctcatagg cggcgttttt
    24121 tcgggtgaag tcggctcgct tgctcaccgt ggaggcgagc tctttcagtt ttgtatattt
    24181 gtccgttcga atcattgcgc cggacgaagg gttctccgaa gcgaatttca tcctccatga
    24241 gagcggattg tatccggtca gcttcgccag ttcactgtag tgggcttcgc tgctggccag
    24301 cgcctcgctg tagccaagcc ccccgtagaa gtgggccggc cggttgttcg tggtatgaac
    24361 ctccacatga atcgtcagcg cggtgaccgg gtagaggggg atcaggccgg tatagagatg
    24421 gtttaccagc tcctcccgga agaaggaggc gcatccctga tcgacgacga cccggatctc
    24481 ctccgcctga ggtttcccgc tgctttggta gtaggtcttt cgagtgattt cgtagcgcgg
    24541 cggggcggcc tccatctcat agaggatttc cgtgtcccgt ccgcttttta tcgccgcaac
    24601 gctgcacagc gcggcgagct gggcggggag aatcaacatc tcgtcatggg gcgcataaaa
    24661 gggttggcgg tggatgatga cgttcttttt ggaaaagccg gtaacctccg ccacgctttc
    24721 acggacgtgg gcgggccact gggtgggcgc ctcgatgtgg agggtttccc cgtcaaacat
    24781 tacggatacg cggttgtacg taaggcgctt tttggttttg gagtggaagg cgtaggaacg
    24841 ttcaagaatg ttgaggtttt cctctttcag cgcctcctcc agattcccga acgtgtagga
    24901 gtatggcgtc acggtcaagg agtccaccgg ttcatcttcg acgtaggtga cggagattcc
    24961 cttggctttg acaagggcac tttcccgatt gggtccgaag agggcgagca gcggttgatc
    25021 gcggtaggtg atcgctccgc tgctgagcaa aggaatctgg gcatcaagga cgcgcaggcg
    25081 attggagccg gggatgtccc ggggggcgac gatgacgaag gagggatccg cttcgggaac
    25141 gacgatcctg tcgaaccgcc cccgagcgat tcgactggtg atcagaactc cttgaagggg
    25201 tgtgagcctg cccattgttg gtcgcttttc cgccataatc ttcctctgat tccaaacgat
    25261 accacgcctg aagcattggg gcaattgcct ctcttgaacg cggcgtatac aatcgaaggc
    25321 atggatacca tagagcgtga gattacaaag ctccggtcgg agcttgaggc atatcaacgg
    25381 gcgtactacg tcgacagccg gccgctggtg gatgattcgg agtacgatcg gaagttcgat
    25441 cgcctgctcg aattgg
//
LOCUS       DAOSLI010000027        23390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2420_length_23390_cov_28.746947, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000027 DAOSLI010000000
VERSION     DAOSLI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 23390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23390
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2420_length_23390_cov_28.746947"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..567
                     /gene="rpsF"
                     /locus_tag="PLF67_05375"
     CDS             286..567
                     /gene="rpsF"
                     /locus_tag="PLF67_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOE89478.1"
                     /translation="MRDYEFTVILVTDEDKTKAGLEAVTAAFAAAGVEITKQEDMGVK
                     FLAYEIKKQDKGHYVYFELKADPASITGFEQEFRLNANILKYLFVNAEK"
     gene            592..1056
                     /gene="ssb"
                     /locus_tag="PLF67_05380"
     CDS             592..1056
                     /gene="ssb"
                     /locus_tag="PLF67_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE89479.1"
                     /translation="MANDMNVVALVGRLTRDSELRYTQGGMAIARFSIAVNRRKRTAD
                     NRWEDEANFFDCAMFGKSAESVNQYLERGRQVSIIGELRQNRWEQDGQSRSKVEIVVN
                     SLQLLSSPSGQRADAQAFGQPAPRQQSDSYQQELPPIEYGGPEQFDDDQIPF"
     gene            1080..1364
                     /gene="rpsR"
                     /locus_tag="PLF67_05385"
     CDS             1080..1364
                     /gene="rpsR"
                     /locus_tag="PLF67_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOE89480.1"
                     /translation="MRDETNDAMMNDMDGRGGRDRRARRPAFRRKVCKFCTSPQGPDY
                     KNPEQLRRYVTERGKILPARITGNCAKHQRAVTREIKRARVLAYLPFEKK"
     gene            1410..2336
                     /locus_tag="PLF67_05390"
     CDS             1410..2336
                     /locus_tag="PLF67_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOE89481.1"
                     /translation="MTLIVSAVSIVLSQLMIGNFLFTIPLIALAPRYLNRRQALMPVG
                     IVALLVALIELIRSGGFPSSESRVLGAVSLFVPAVLLVGSAVWITLDGYRLLYRYLAS
                     CLFAVVASISIVIWFGSAGEAILRVDSMMSESFRTLFAQFSGSAIQYDATMNLLYRTM
                     VMILGAVLAPMMMAIMGFAFYVALSFQSRGSEEPFVVRFSRIRLPENGIWVFLSGWTL
                     VLVFVLVKSGYLPRALAIQCALSISLLYAIQGFAIILFHLVKRNLVHRAGRLGFMVVL
                     LMFLVPGLNLLVVFALPLLGVTETWIAYRRNV"
     gene            2348..2956
                     /gene="rplI"
                     /locus_tag="PLF67_05395"
     CDS             2348..2956
                     /gene="rplI"
                     /locus_tag="PLF67_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOE89482.1"
                     /translation="MKIILNQDVANLGEEGDIVVVKAGYARNFLLPKGMAVLHNKANL
                     AIFASRAEAIEKRKALKRKESASLRDKLSELELKIVVTAGESGRLFGSVTSQMVQNEL
                     SKLGYEIERKKIEVPTHTIKMTGTYTIHVRLYEGDVADVKLVVASESVEKAKAAQQAK
                     EAAEAAKAAEKAAAEAAAAAAAEEVVEEEEAVEEAAAEEDNE"
     gene            2962..4347
                     /gene="dnaB"
                     /locus_tag="PLF67_05400"
     CDS             2962..4347
                     /gene="dnaB"
                     /locus_tag="PLF67_05400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOE89483.1"
                     /translation="MATGSIQGRVPPQNEEVERAVLGAILLNDRVLTDVANVLAPGDF
                     YKVAHQQLFAAILAYKQESSEVLDLLTLNAFLKSKALVDQCGGPAYIASLTSDVPSTT
                     NAVYYAQILKALSYRRKLLLYASQLRDRAFDESQDIQKVIDDGEQELSVLSNEAAGSD
                     SYHDIKNLVNTLISDIEERSITGEKRGFDSGFSALDSITGGFKKQEFIIIGARPSIGK
                     TAFALSIVLNMLIKKRIRVGFFSLEMSAFSLMERLLSGHSRVDFTHIRRATLQNREMQ
                     AIVDASAALYDSELYIQDTPNMKLMELRAQARRMKLEHDVQVIFIDYIGLMDAETDSR
                     VPRHEQISIISRSLKQLARELKVPIICLSQVGRQADGIEPKLADLRDSGSIEQDADVV
                     ILLHRDTTKYRTDDEEQRERNNVQETKVIMAKNRNGETGSFSIAFVRNIVRFEDLVTT
                     RPYTAAANPNA"
     gene            complement(4337..5716)
                     /gene="rseP"
                     /locus_tag="PLF67_05405"
     CDS             complement(4337..5716)
                     /gene="rseP"
                     /locus_tag="PLF67_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOE89484.1"
                     /translation="MSYFLAQVIKYLIGIVGITVVVLIHELGHLVAARICGIDVEVFS
                     FGFGPRLIGKHIGDLDLRLSLFPVGGYCRLKGSDDVRQSLRNEQSLFLEEGSIFAASP
                     IRRIITYLAGPVASFLLALLIYTILSLMPYEVLSTEAIVTTVNDYPELFGPEATSPAY
                     DAGIRSGDRVLALNGETIDDWEALETILPVSPEHLFMVERNGQSFHYWVEGEKQADGT
                     YRYGLAVLQEAIVGNVRSLSAEKEAGLKKGDRIIASQGTVVHNHLDLLSTLRGELSRV
                     EFTVLRDGEEVRLSFYPRRGDDGKPIFDFSLLAATKHVKPTSKALTSGVGQTSKILHQ
                     TLDSIRSLFTGTQKDLRSNVTGVTRSALLIGDIAALGFEQETKQGLHALLYLLGVVSI
                     SLAVVNLLPLPAFDGIQIMVAFLALITGHTIKMRTYYVLQVAGVLFIIVVFIALAGSD
                     LRYLLSLRR"
     gene            complement(5713..6816)
                     /gene="dxr"
                     /locus_tag="PLF67_05410"
     CDS             complement(5713..6816)
                     /gene="dxr"
                     /locus_tag="PLF67_05410"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOE89485.1"
                     /translation="MKRIAILGATGSIGTTALSVLRNRTDLPLKVISLSAHNNRERLL
                     ALANEFSSEALCFTGSKEEGFFHGYEGLKAMLEEYPVDIVLNGIAGFDGLKASVAVLE
                     AGYDLALANKESVVCGGELLFELARKHNAKIIPVDSEHSAIYELLRGRERKDVSSLIL
                     TASGGPFRSRDLSTFDSISVGDALAHPTWQMGKKISIDSATMANKALEVIEASYLFGF
                     QGDAIEVVIHPQSIVHSMIRTRDGAVYAQLGNPDMSLPIINALTSMSSNLVSPLDFNH
                     LMLTFEAIDEKRFPLLRCGYDALSGQGGYPLAFNAANEVAVEAFLSERITYPRLIHTV
                     QRTLDHDWTFTPKSVEEIIALDSEVRSKAKELM"
     gene            complement(6813..7661)
                     /locus_tag="PLF67_05415"
     CDS             complement(6813..7661)
                     /locus_tag="PLF67_05415"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOE89486.1"
                     /translation="MNSTIKRVLTTIIAPPLLFALIFFLPHASYAALSVLAVLVTIGG
                     AYELRNLSNSAHGTTGGMPVVITAFLPLAQWVQNHCLPDVEVTFFMFTILSLALCVME
                     LSKGREDAYAASILRLANGILILFYPGLLITFIQRITVFQAPGALLVLFFLIIFGNDI
                     FAYVFGVWLGKNNRGWASVSPNKSIAGLVGGILSAVVLGGLFVFFVPALSAALSLPKA
                     IILSVLTAVVANIGDLIESAFKRSAKMKDSGTLIPGRGGLLDSIDSMLVAAPVFYLLL
                     AGFGVQ"
     gene            complement(7658..8350)
                     /gene="uppS"
                     /locus_tag="PLF67_05420"
     CDS             complement(7658..8350)
                     /gene="uppS"
                     /locus_tag="PLF67_05420"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOE89487.1"
                     /translation="MSDDRLGLKHLGIIMDGNGRWAKKRNLPRTAGHLEGLKALKRVI
                     IEAANQQIEWVTFYAFSTENWKRTEEEVSYLMRLFTSKLLGELAFYRAHQIRILTMGD
                     LAGLSSEVQNVITETVEATKDHKGITAVIAINYGGRDEIVRSVNRLIASNRQVSVEAI
                     TSNVDLPQAPPVDMIVRSAGEMRLSNFLLWESAYAELGFYEPLWPDWGAPEIQLIVRD
                     YAKRVRTYGGIE"
     gene            complement(8354..8908)
                     /gene="frr"
                     /locus_tag="PLF67_05425"
     CDS             complement(8354..8908)
                     /gene="frr"
                     /locus_tag="PLF67_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOE89488.1"
                     /translation="MHNVLESCETKMKKSVESLQREFATLRTGRASASLLDRIRVDYY
                     GAETPLNQVSTISTPEARTIVIQPWDKSLLGVIEKAILKSDLDLPPNNDGKLIRLNFP
                     PLNEERRKALVKTAKSIAEQSRVAIRNIRREAIDDLKKLEKSSEISEDEQRDGETRVQ
                     KLTDSYIEQINALSDEKEKEIMEI"
     gene            complement(8966..9817)
                     /gene="tsf"
                     /locus_tag="PLF67_05430"
     CDS             complement(8966..9817)
                     /gene="tsf"
                     /locus_tag="PLF67_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOE89489.1"
                     /translation="MVITADLVKKLRDATGAGMMDCKKALTEANGDFAEAEKILKKMG
                     LAAVAKRQDRATDNGRVFVKVAKNKAILINLTCETDFVATNKEFIALGEKICDVALEK
                     GYTEINDELNGMVNDLISIIKENMSLANLTVYDVGADELASTYTHGEGSLAVLVKIKA
                     GDPAVFEKEEVKEFANDLALHVAAFTPSYLSREKVDEKYIAEQVEIFTAQAAKLDKPA
                     KVVEGIVKGKLNKHLSEICFLDQAFVKDDTVTVAQKAKEIAKAVGTTIEIVDYAYLKA
                     GWASCAI"
     gene            complement(9830..10723)
                     /gene="rpsB"
                     /locus_tag="PLF67_05435"
     CDS             complement(9830..10723)
                     /gene="rpsB"
                     /locus_tag="PLF67_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOE89490.1"
                     /translation="MSVVTMKSLLESGVHFGHQTKRWNPKMARFIFSERNGIHIIDLQ
                     KTSACIIEAYDAVRGVVKQGKSVLFVGTKKQAQQAIETEAKRCGMPYVNHRWLGGMLT
                     NFSTIKKSIATLKKIERMENDGTFNALTKKEISLLEKQKEKLEKNLGGIKDMKELPGA
                     IFVIDTKKEAIAIAEATRLGIPVIAVVDTNCDPTYITYPIPGNDDAIRAISLFVEIIA
                     NAVIDADNEAGIQIIESLGDDDEPIEEPEVSKKDDDDDDEAIVYSTDDDADYSKFEDE
                     EEEEDDRTDEDDDEDETLYND"
     gene            complement(10793..11365)
                     /locus_tag="PLF67_05440"
     CDS             complement(10793..11365)
                     /locus_tag="PLF67_05440"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOE89491.1"
                     /translation="MSKTIILASKSLGRKLLLEEAGFEVLIQPSKADEEHQRESVEKS
                     VRLLAMRKLEAFLRDRPSPTHPVIASDTLLAFEEIELGQPETREAAFSQLRLLSGTTH
                     HVYSAWALWVNGDVHQGCDRCAVTFKELSDDEIEAYLDTGEWVGAAGSYRYQGRGKEL
                     VRSTTGDESTVIGLPMNQIFGILAKALPDW"
     gene            complement(11358..13277)
                     /locus_tag="PLF67_05445"
     CDS             complement(11358..13277)
                     /locus_tag="PLF67_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOE89492.1"
                     /translation="MATLQVQRLSIAFADRQLLNDISFTLSERARTALVGANGSGKST
                     LLQIIAGLVSADIYECAHPKEMRISYLPQSDIVLEDGTAFEQVELAYNRFLPLVDEQH
                     SIEERLATGELSDYLLQRLHEIQETLLDEGYHDRKTEIAIILKGLGFHDDDLHRPCSE
                     FSGGWQMRIALAKVLAEKPHLLLLDEPTNYLDIEARYWLRNYLRVYPESVMIVSHDRS
                     FLDETVNEVFELFHGQLKRYKGTYSQYEEQRELEIQSLIHAYEQQQEEIKKQKEFIER
                     FRAKASKARQVQGRIKQLEKLEIIEIPEHLRNLSFSFPPAPHSGKQVLKIENLTKRYG
                     SLTVFEGFDSIITKGERIAVVGKNGTGKSTLLRILAGIDTDYQGSLTVGSGVRIGYFA
                     QDAKQALDDSNTVLDELASVASTADIPRLRTYLGSFLFSGDDVFKRVGILSGGERSRL
                     ILLKTLMHPVNLLILDEPTNHLDIAAKKALLQAIREYDGTLIFVSHDAYVIQEAATSI
                     LYLTEENPPELFPGDWSYFSYRLEQKEAIPAEAAKEEKKPARSYREERQKHNRLVSLR
                     RQAEESLLTLDVIETELQEVQRLMSQPENYSVEEKILKLVKEEERLRKNLADEEAAWL
                     TLEEERMILEEELYE"
     gene            13383..14867
                     /gene="aroE"
                     /locus_tag="PLF67_05450"
     CDS             13383..14867
                     /gene="aroE"
                     /locus_tag="PLF67_05450"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOE89493.1"
                     /translation="MLCLTLTASTLAENKALVEKNRSWIGAAELRLDHLDEDELKVAS
                     AFPSTVDLPLILTYRRASDGGLCTKNEKDRLQTLYEAAKGGWAYVDIEEDVKKSDLKC
                     KDPLFEGRVDLEESLRARNIRIIRSYHDFEGVPEDLFGRVNRLAGKGDIPKVAVTPQS
                     LLDVIMLFQVEEQLAHIKEKIIIGMGQLGIPTRILYKRLGSMLSFVSDNQAAPGHLGP
                     REMSEVYHADRIDRNTHIYGVIGNPVYHTSSPKIHNRGFEAIRYNAVYVPFLVDSVRS
                     FFKLAELLKIRGFSVTIPHKQAVVPYLGKTSREVKQIGSCNTVVRIQGMWKGINTDYY
                     GFLTPIQDRIASGSIKSALVVGSGGASRAIVYALINHGVKVTILNRTVERARKLGDET
                     MSGYDSLENAHLYQGSVDLVVQTTSVGMGELEGTNPIPSFAFTGKEIAYELIYRPEET
                     AFLQAAKRAGCEPVFGKEMLIEQGKLQFEAFCGYHFPHWIPLDL"
     gene            complement(14864..16726)
                     /locus_tag="PLF67_05455"
     CDS             complement(14864..16726)
                     /locus_tag="PLF67_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89494.1"
                     /translation="MSHEQLKTLLEQYTDDEYFFLARNYLGLVESPFHKEHISSRLIA
                     FFALEENRKKMLSLIDSFDQMIITALLFTGGLRFEQLATLLEGIHPYGKLLLRVANLR
                     ERMIIIATGNTLVPNPLLEEHFYRFAGLTPLFGVEEGTVHTHPFSYREFLQAILTLAE
                     RPGRSFFRKEYETLFPTMASDDLPSIFETIHSSFVKLGIIIEDPQRRVDWQRAHDLFL
                     LDEQQIKAMMLAGHMEGALNTCCADLVFLLEDIGTFSASGLKRLSQALAFKHHVKRCD
                     LTSVLLGLGLLVKTKGGYNHVSYTQGEPASSFIVDSDYSVSYLGSLDADSLLVRFAAL
                     ETLDRTRLWRITEERLRSALDGGVGYDEIRSYLKTYVNGTLNTALLKSLDLIAERSRQ
                     VQIYDGLVLHTDERVGRIVEQLPALQEHILDRLSPTIFLMKRSSERIWRQILTQAGLS
                     LGSTKGELAHGSDGETRELPALQWPKEFQKPKKFKKSVTLPPVVPDEKLREAIIRKNY
                     PKEVEEDLLERFEERLIINKSQITPQILYTRIEAGGFDYQGKISLAKQASSKEGTVVQ
                     LFMGGEELMALALELTYTPKKEALFKVAILPQMETRVIPLSKIFLLRQIKMLFL"
     gene            complement(16723..18381)
                     /locus_tag="PLF67_05460"
     CDS             complement(16723..18381)
                     /locus_tag="PLF67_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HOE89495.1"
                     /translation="MKDTPLIVQSDRSLLLDVHHPDSEACRKDLIRFCELVKSPEHMH
                     TYRISEISLWNASGEGLDGEAIVEMIKKWSKFPPPESVLFFVRDIAGRWGSLVLTEST
                     DEAYYLLTISIEKIRLEIKHRKELMKILVVKDEDSFLVERYLRGELKLRLIKLGYPVD
                     DRIPLEKGPPLMFDKRKTTLGGQPFIVRPYQSQAADALLGDLGRGRGFGTIVLPCGSG
                     KTIVGLEIMSRLKTSTLIVTTNVVAVHQWMREIIDKTTLESEDVGEYTGNLKERKPIT
                     VCTYQVLTYRKSKTAPFAHMDLLTKGRWGLIIYDEVHMLPAPVFKVTAELQATFRVGF
                     TATLVREDGREDEVFSLVGPKRYDVPWIELQQQGFIAQAYCHEIRLDLPKELELEYAL
                     ATKRQKYRIASENPLKIEVVRSLIERHSDDFILIIGQYIDQLEEVAKLFDLPIITGST
                     PNAKRDELYNAFRNGEIRILVVSKVANYAIDLPDASVAIQISGTFGSRSEEAQRLGRI
                     LRPKGRSSFFYSVITRYSSEEEFSANRQKFLAEQGYSYEIEVWE"
     gene            complement(18378..19430)
                     /gene="aroC"
                     /locus_tag="PLF67_05465"
     CDS             complement(18378..19430)
                     /gene="aroC"
                     /locus_tag="PLF67_05465"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOE89496.1"
                     /translation="MGHNTFGNHFSVTTFGESHGPALGVIVDGVESGFTVDLEAIQKE
                     MDRRRPGGNPSGTERNEIDKLVILSGLYEGVTTGTPIAMILESTNQRPKDYSHLKDVF
                     RPGHADWTFYKKYGVRDIRGGGRSSGRETSARVAAGALAKQLLAKRGITIRAGVVQVG
                     DIVAMKRDWEDPDPILACPDREAAASMVRLIETVRKENDSIGGVIECIVRGAPVGLGE
                     PVFGKLDALLAHAILSIGAVKGIQFGSGFALSAMRGSQANDQRTKDGFLSNHSGGILG
                     GISSGQDIVFQAAIKPTSSIGIGQRTVDKEGNDVTLTVTGRHDPCICGRAVVVIEAMT
                     AITLLDLYYTAFGKDA"
     gene            complement(19433..19948)
                     /locus_tag="PLF67_05470"
     CDS             complement(19433..19948)
                     /locus_tag="PLF67_05470"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOE89497.1"
                     /translation="MSHIFLCGIKHSGKTTIGKLLAKRLGRRWYDLDREIERKIGEPI
                     RTFYRAFGQEAFQQLEVEVLEAIIGEENLVISLGGGAADNDPLMKMVKERGTLVYLYL
                     EEAHLLERILRGGIPPFLDEEDVEGSFHRLYERRNRRYSKDCNHLIQLPFDQAKEENV
                     ELVLSSLGLKE"
     gene            complement(19950..20420)
                     /locus_tag="PLF67_05475"
     CDS             complement(19950..20420)
                     /locus_tag="PLF67_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89498.1"
                     /translation="MVAIYVLPLLTLLLNFLAFGSCLRFLFSRQGLYWFIPLLLTLFL
                     IVPNALTLYTVASDPNSFISTGGILTYQPLGLSLLWYLIIITFHYALKKTIRINRYEA
                     DMRKNLHEARYQAKIESRQLADREKSRKERFAGNRSVVPRTNTHPLAWVELFED"
     gene            20486..21637
                     /locus_tag="PLF67_05480"
     CDS             20486..21637
                     /locus_tag="PLF67_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE89499.1"
                     /translation="MKLHNEIDLELLIHRNRPREIDVRGPFYRDTTAIIHSSAFRRLK
                     HKTQVFFAPSNDHICTRMEHCLHVASIASTICKGLGLDSDLAWAIGMGHDLGHTPFGH
                     TGEKILSRMMEQHGLGNFEHEKHSLRVVDFLSEQGRGLNLTYAVRDGIAFHNGEHLVK
                     SIRPTFEVRDLTRDLRNLTILPATYEGAVVRFSDTIAYLGRDYEDACRLGVIDKEALP
                     PRVATRLGKTNARIIDTLVNDLILHSNPVEGISFSDELFPVIEEMMEFNYREIYKSPM
                     LEGYERYFERLFTLIISYLETLLSECGDDERCYKDERNMLAMGFYHHKEEMQQAYLAH
                     DGNPDRLIYDYIAGMSDHFALDCANEILKPEHLNDSIEQSLTGKWFDVR"
     gene            complement(21634..22098)
                     /gene="nrdR"
                     /locus_tag="PLF67_05485"
     CDS             complement(21634..22098)
                     /gene="nrdR"
                     /locus_tag="PLF67_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOE89500.1"
                     /translation="MRCPRCSSLEDKVLESRQNASGTAIRRRRECLSCGYRSTSYERI
                     EDKPVMVIKRDGRREMFDISKVGRGIRSCTEKLSISEHQIETLLQNIEDAIMLKLGNR
                     REISSTEIGEEALKQLKTVDLVAYVRFAAVYKAFNDIEQFIGEIKQLDKELN"
     gene            22305..22964
                     /locus_tag="PLF67_05490"
     CDS             22305..22964
                     /locus_tag="PLF67_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89501.1"
                     /translation="MAESVSSCVKRIVDKSPFIGEMLTLGILSFSNYAALISDEVERA
                     LGKPAKSSAIVMALRRYGEDLKRSNLVRDTSNVNYQIVMKTNIFDINLVRRDSFIGKL
                     GSLYNQISTEKGDFLNITLGSHEISLAVSEKYRPLIKELTKDEEVLNKEDDLVALSLV
                     FTGDFLQTPGIVYEAVRHLAWQQINVIEIVSTMNELTFVISREDSMRAFDVLQNFLRV
                     Q"
     gene            22954..>23390
                     /locus_tag="PLF67_05495"
     CDS             22954..>23390
                     /locus_tag="PLF67_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOE89502.1"
                     /translation="MFNKLTLIVSPIGGGKTRYLSNLNLPSPHCGVVTSSNIEKTIYH
                     IKDLAGGEERLLLSEAYLPNARRFGRFFVLEETFDWANERIISNLEASKAVVFDEIGR
                     IEIEGWGLKSSFLKALRTPAIEIYAAVRDTLLDQVLKTFDLEE"
BASE COUNT         5266 a         6318 c         6372 g         5434 t
ORIGIN      
        1 ggtaatcaaa accgcggggc tttgcccctc aggaaggagc tctacagtgc cttgtggacg
       61 aaaaagaaaa agacataaga ttgccacgca caagcggaag aaaaagctga gaaagaaccg
      121 tcacaagaaa aagaaatagg ttcgatggca atcatcgtag ggaaaccgcc ggatattctg
      181 gcggtttctg ccgttttgtt gacctggacc ggtcaagcat gatagattac aaagacctct
      241 ttatccatcc ttgaggatgg atcatataga ccataaggag gaaccatgag agattatgag
      301 ttcaccgtta ttctcgtcac ggacgaggat aaaacaaagg caggattgga agcagtaacc
      361 gccgcctttg cagctgccgg ggtagagatt accaagcagg aagacatggg cgttaaattt
      421 cttgcctatg aaatcaagaa gcaggacaaa ggacactacg tctacttcga attgaaggct
      481 gatcccgctt ccatcaccgg ttttgagcaa gagttcagac tcaacgccaa catccttaaa
      541 tatctgttcg taaacgccga gaaataaccc gaaaagggat tggagtgcaa tatggcaaat
      601 gatatgaacg tagtcgcatt agtaggacgc ctgacccggg acagcgagct tcgctacacc
      661 caaggcggga tggcgatcgc ccgattctcg atagcagtca accggagaaa gaggacggct
      721 gacaaccgct gggaggatga agcaaacttc ttcgactgcg cgatgttcgg aaagagtgcc
      781 gaatccgtaa accagtatct ggagcggggg aggcaggttt cgatcatcgg cgaactgaga
      841 cagaaccgct gggaacagga tggtcaaagt cgcagtaaag tggaaatcgt tgtcaattca
      901 ttgcagcttc tttcgagtcc aagcggccag cgagccgatg ctcaagcgtt tggtcaacct
      961 gcaccgaggc agcaaagcga cagctaccaa caggaactgc caccgatcga atacggtggg
     1021 cccgagcagt ttgacgacga tcagattccg ttctgattcc attcaatagg agaaatgtaa
     1081 tgcgcgacga aaccaacgac gcaatgatga atgatatgga tggacgcggt ggccgcgatc
     1141 gccgtgcccg ccgccccgcc tttcgaagaa aagtatgcaa gttctgtact tcgccccagg
     1201 gccccgatta caagaatccc gagcagcttc gccggtatgt taccgagcgc ggcaagattc
     1261 ttcccgctcg catcaccgga aactgcgcca agcaccaacg ggctgtaacc cgtgaaatca
     1321 agcgtgcccg ggtgcttgcc tatcttccgt ttgagaagaa atagatcagg attgccatgg
     1381 accaaagcca acgtgcgccg attgtgaaga tgaccctgat tgtaagtgcg gtaagcatcg
     1441 tgctttccca acttatgatc ggcaactttc tcttcacgat tcccttgatc gctctcgccc
     1501 cccgatacct caatcgcaga caggctctga tgcccgtcgg gattgtcgct cttctggtgg
     1561 cgctcatcga gctcatccgc tccggcggtt ttcccagcag tgaaagccga gtcctcggag
     1621 ccgtatcgct ctttgttccc gcggtcttgc tggtcggcag cgccgtttgg attaccctcg
     1681 atggataccg ccttctctat cgttaccttg cttcctgcct ctttgcagtg gtggcatcga
     1741 tttcgatagt gatctggttc ggctcggccg gggaagcgat attgcgtgtt gacagcatga
     1801 tgagcgagag tttcagaaca ctctttgctc aattcagcgg atcggccatc caatatgacg
     1861 ctacgatgaa tcttctctat cgcacgatgg tgatgatcct cggagcggtt ctcgctccga
     1921 tgatgatggc gatcatggga tttgccttct atgtcgcatt atcttttcag agccgcggca
     1981 gtgaagagcc gtttgtcgtc cgtttcagcc gaattcgtct gccggagaac gggatttggg
     2041 tctttttgag tgggtggacg cttgtgcttg tctttgtact tgtcaagagc ggctacctcc
     2101 cccgtgccct tgcgatccag tgtgctcttt ctatcagctt gctgtatgca attcagggct
     2161 ttgcaatcat cctgtttcat ctggtgaagc gaaaccttgt ccatcgagcc ggtcggttgg
     2221 ggttcatggt tgtactgttg atgtttttgg tgccagggtt gaacctgctg gttgtcttcg
     2281 ctcttcccct cctgggagtg acggagactt ggatagcgta tagaagaaat gtgtaaggag
     2341 tattcctatg aagattattc ttaatcagga tgtcgcgaac ctcggtgaag agggcgatat
     2401 tgttgttgta aaagccggat atgcccgcaa cttcctcctg cctaaaggca tggcggtgtt
     2461 gcacaataaa gcaaaccttg cgatcttcgc ctcccgggcc gaagcgatcg agaaacggaa
     2521 agcgcttaag cgcaaagaaa gcgcttcgct tcgtgacaag ctcagtgagc ttgagctcaa
     2581 gatcgtcgtt accgccggtg aaagcggccg tcttttcggt tccgttacca gccagatggt
     2641 ccaaaatgag ctttccaagc tcggatacga gattgagcgc aaaaagatcg aggtgccgac
     2701 ccatacgatc aaaatgaccg gtacgtacac gatccacgtg cgtctctacg aaggcgatgt
     2761 cgccgatgtg aagcttgtcg tcgccagtga aagcgtggaa aaagcaaaag ccgctcagca
     2821 ggccaaggaa gccgctgaag ccgccaaggc ggcggaaaag gcagctgccg aagcagccgc
     2881 cgctgccgca gcggaagagg ttgtcgaaga agaggaagct gttgaagaag cggctgccga
     2941 ggaagataac gagtaagaga tatggctaca ggcagcatac aaggccgagt tcctcctcaa
     3001 aacgaagaag ttgaacgagc ggtccttgga gcgattcttc tcaatgaccg ggtgttgacg
     3061 gatgtcgcca acgtccttgc tccgggagat ttttataaag ttgctcacca acagctgttt
     3121 gcagccattc ttgcgtataa gcaggaaagc tcggaggtct tggaccttct cacgctgaac
     3181 gcctttctca aaagcaaggc gctcgtcgac caatgtggcg gacctgctta tattgcaagc
     3241 ttgaccagcg atgttccctc aacgaccaac gctgtgtatt acgctcaaat cctcaaagcg
     3301 ctcagctacc gaagaaagct tctcctgtat gcttcacagc ttcgagaccg ggcgtttgat
     3361 gaatcgcagg atatccagaa agtcatcgat gacggtgagc aggagctgag cgtcctctcc
     3421 aacgaggcgg ccggcagtga ttcctaccac gatatcaaaa atcttgttaa taccttgatc
     3481 tccgatatcg aggaacgttc aatcaccgga gaaaaacgag gcttcgacag cggattttcc
     3541 gccctcgact cgatcaccgg agggttcaag aagcaggagt tcatcatcat cggcgctcgc
     3601 ccttcgatcg gaaagaccgc cttcgctctg tcgatcgtgt tgaatatgct gatcaaaaag
     3661 agaatccggg tcgggttctt ctcgcttgag atgagtgcgt tcagtctgat ggagcgcctc
     3721 ctgtcgggcc acagccgagt cgatttcacg catatccgac gggcgacgct ccaaaacagg
     3781 gagatgcagg cgatcgtcga cgcttccgcc gcgctctacg attcagagct ctacatccag
     3841 gacacgccga acatgaagct gatggagctc cgcgcccaag cgcgaaggat gaagcttgaa
     3901 cacgatgttc aggtgatctt catcgactac atcggcctga tggacgctga gaccgactcg
     3961 cgggtccccc gtcatgaaca gatctcaatc atctcacgat cgttgaaaca gttggcccgg
     4021 gaattgaaag tcccgatcat ctgcctttcg caggtggggc gccaagcgga tggaatcgag
     4081 ccgaaactgg ccgatctgcg cgactccggc tcaatcgagc aggatgcgga cgtcgtgatc
     4141 cttcttcatc gggataccac gaaatatcgg accgacgatg aagaacagcg ggaaagaaac
     4201 aacgtccagg aaacgaaggt gatcatggcg aagaaccgaa acggagagac aggaagcttt
     4261 tccatcgcat tcgtccgcaa tatcgttcgt tttgaagact tggttacgac ccggccgtat
     4321 accgccgcgg caaatcctaa cgcctgagcg aaagaaggta tcgaagatcg gagcccgcaa
     4381 gggcgatgaa caccacaatg atgaagagaa cccccgccac ctgaagcacg taataggtcc
     4441 gcatcttgat cgtgtgaccg gtaatgagtg caagaaatgc gaccatgatc tgaattccgt
     4501 cgaaagccgg cagcggaagc aggttcacca ccgccagact tatcgaaacc accccgagca
     4561 gatagagcag ggcgtgaagg ccttgtttcg tttcctgctc gaagccgagc gctgcgatat
     4621 caccgatcag gagcgccgaa cgggtaactc cggtaacatt ggagcgcaga tctttctgag
     4681 ttcccgtaaa gagggatcgg atgctgtcaa gggtttgatg gagaatcttg gaggtttgcc
     4741 cgacgccgga ggtcagtgcc ttgctggtgg gtttgacatg ttttgtcgca gccaacaggg
     4801 agaaatcgaa gatcggcttt ccatcgtcgc cgcggcgggg atagaaggag agccgcacct
     4861 cttcaccgtc ccgcagaacc gtgaactcaa cccggctcag ttccccccgg agggttgaaa
     4921 gcagatccag atgattatga acgaccgtcc cctgagaggc gatgattctg tctccctttt
     4981 tcagtccggc ttccttttcc gccgagaggg aacgaacgtt gccgacgatc gcttcctgga
     5041 ggacggcaag cccgtaccga tacgttccat ccgcctgctt ttccccttcc acccaataat
     5101 ggaaggattg cccgttccgt tcgaccatga acaagtgctc aggtgaaacg ggcaggatcg
     5161 tctccaatgc ttcccagtca tcaattgttt ccccgttgag ggcgaggacg cggtcgccgc
     5221 tccgaatccc cgcatcatag gcgggactcg tcgcttccgg accgaagagc tcgggatagt
     5281 cgttcaccgt cgtaacgatc gcctcggtgg aaaggacctc gtacggcatg agcgagagga
     5341 ttgtgtagat aagcagggca agaagaaacg aagcgaccgg acctgcgagg taggtgatga
     5401 tccgcctgat tggtgatgcg gcgaaaatcg aaccctcttc caggaaaaga gattgttcat
     5461 tcctcagcga ttgccggaca tcatcgcttc ctttgagccg acagtatccg ccgacgggaa
     5521 agagcgagag cctcaaatca agatcgccga tatgtttgcc gatgagacgc ggaccgaagc
     5581 caaacgagaa gacttcaacg tcgatgccgc agattcgggc ggcaacaagg tgcccgagct
     5641 catgaatcaa cacgacaacg gtgattccaa cgatgccgat gaggtatttg atcacctgag
     5701 ccagaaagta gctcacatga gctccttggc ttttgagcgg acctcgctgt cgagggcgat
     5761 gatctcctcc acggattttg gagtgaacgt ccaatcgtgg tccaaggttc gttggactgt
     5821 atgaatcagt cggggatagg tgattcgttc cgataaaaac gcctcgacgg cgacttcatt
     5881 tgccgcattg aacgccaaag gatatccccc ttggccggat agggcgtcat agccgcagcg
     5941 aaggagggga aagcgcttct catcgattgc ttcaaacgtc agcatcaggt ggttgaaatc
     6001 cagcggagat accagatttg agctcatcga cgtaagcgca ttgatgatcg gaaggctcat
     6061 gtcgggattc cccaattggg catagaccgc tccatcccgg gtacgaatca tcgaatggac
     6121 gatcgattgc gggtggatga cgacttcgat cgcatcacct tggaagccga agagatagga
     6181 agcttcaatc acttccaacg ctttgttcgc catggtggct gaatcgatcg agatcttttt
     6241 ccccatctgc caagtggggt gggccaacgc atcgccaacg ctgatcgaat caaaggtgga
     6301 aagatcgcgg cttctaaacg gtccgccgct ggcggtaaga atcagggaag agacatcctt
     6361 tcgttctctt cctctcaaga gctcatagat cgccgaatgt tcgctgtcca ccggaatgat
     6421 ctttgcgttg tgcttccttg ccagctcaaa gagaagctca ccgccgcaca ccaccgattc
     6481 cttgtttgcg agggcaagat catagcctgc ttcgagaacg gcaacggaag ctttcaagcc
     6541 gtcgaaaccg gcgatcccgt tgaggacgat atccaccgga tattcctcaa gcatcgcctt
     6601 cagtccttca tagccgtgga aaaatccctc ttccttgcta ccggtaaagc agagcgcttc
     6661 agagctgaat tcattggcca gagcgaggag gcgttccctg ttgttgtggg cggagagcga
     6721 gatcaccttc aagggaagat cggttcggtt gcgcaacacc gaaagggcgg tcgttccgat
     6781 cgagccggtg gccccgagga tcgctatccg tttcattgca cgccaaagcc ggcgaggaga
     6841 agataaaaga caggggcagc gacgagcatc gaatcgattg aatcgagcaa gcctcctcgg
     6901 cccggaatca aggtgccgct gtccttcatc tttgcggatc gtttaaacgc cgattcgatc
     6961 agatcaccga tattggcgac aaccgcagtg agcacactga gaatgatcgc cttggggagg
     7021 gagagcgcag cggagagcgc cggtacaaag aacacaaaga gtcctccgag aaccaccgcc
     7081 gaaagaattc cacccacaag gcctgcaatc gacttgttgg gacttacgct cgcccaaccg
     7141 cgattgtttt tacccaacca taccccgaaa acatacgcaa agatatcatt gccgaagatg
     7201 atcaggaaga agagcaccaa caacgctccc ggggcctgga agaccgtgat ccgctggatg
     7261 aaggtgatga gcaggcccgg gtagaagaga atcaatatcc cgttcgccag ccggaggatc
     7321 gaagcggcat acgcatcttc cctgcctttg gacagctcca tgacgcagag cgccaaggat
     7381 aggatggtga acatgaaaaa cgtcacttca acatcgggaa gacagtggtt ctgaacccac
     7441 tgggcaaggg gaagaaaagc ggtaatgacg acgggcattc cgccggtcgt tccatgagcg
     7501 ctgtttgaga gatttctgag ttcgtatgct cccccgatcg taaccagcac cgcaaggacg
     7561 ctgagggctg cataggaggc gtggggaaga aaaaaaatca gggcgaagag cagcggaggg
     7621 gcgatgatcg tggtcaatac ccgcttgatc gtcgagttca ttcgatccct ccataggtcc
     7681 gtacccgctt ggcgtaatcg cgaacaatca gttggatctc cggtgcgccc caatccggcc
     7741 agaggggttc ataaaacccc aattcggcat atgcgctctc ccagagcagg aagttgctga
     7801 gccgcatctc accggcgctt cgcacgatca tatccacggg cggagcttga ggcaagtcca
     7861 cattggaggt gattgcttcg acggagactt gcctgtttga ggcgatcagt cgattcactg
     7921 agcgaacgat ctcatcccgg ccgccgtagt tgattgcgat cactgcggtg attcctttgt
     7981 gatccttcgt tgcttcaacg gtctcagtga tcacgttttg cacttccgag gagaggccgg
     8041 caagatcacc catcgtcaaa atccgaatct gatgggcgcg ataaaaagcg agttcaccga
     8101 gcagtttact ggtgaacagc cgcatcagat aggatacttc ttcttcagtg cgcttccagt
     8161 tttccgttga aaaggcataa aacgtaaccc attcgatctg ttggttcgcc gcttcaatga
     8221 taactcgctt cagcgccttc aatccctcaa gatggccggc ggtcctgggc aggttccgct
     8281 tcttcgccca ccgtccgttt ccgtccatga ttatcccaag gtgtttcaga ccaagccggt
     8341 cgtcactcat ccgttagatc tccataatct ccttctcttt ttcatcagag agggcgttga
     8401 tctgttcaat gtaggaatca gtcaactttt gaactcgagt ctcaccgtcc ctttgctcat
     8461 cttcactgat ttcactcgat ttctcaagct ttttcagatc atcgatcgct tcgcggcgaa
     8521 tatttcgaat ggcgacgcgg gattgttcgg cgatcgactt ggcggttttc accaacgcct
     8581 tccgccgctc ctcattcaat ggggggaagt tgagccggat aagctttccg tcattgttcg
     8641 gcggtaggtc cagatcgctt ttcagaatcg ctttttcgat gacgccaagc aggcttttat
     8701 cccatggttg aatgacgatc gtgcgggcct cgggggtgct gatcgtcgaa acctgattga
     8761 gcggggtctc cgcaccgtag taatcgactc taatccgatc aagcaaagaa gcagatgccc
     8821 ggccggtccg caaggtagcg aactcgcgct gaaggctttc caccgacttc ttcatttttg
     8881 tttcgcagct ttccaatacg ttgtgcatga ctcctccaat aaagccacca ctcacggaag
     8941 tgagtggtgg ttcttcattc ttgtcttaga tggcgcagga agcccaaccg gctttgaggt
     9001 aggcgtagtc gacgatctca atcgtcgtgc cgaccgcttt ggcgatctcc ttggctttct
     9061 gggcaacggt aaccgtatca tccttcacaa aggcctgatc caggaagcaa atttcgctta
     9121 agtgtttgtt cagcttgcct ttgacgattc cctcaacgac ctttgccggt ttgtcgagct
     9181 tcgcagcctg agcagtgaag atctcaacct gttcggcgat gtacttctca tccaccttct
     9241 cccgactgag gtaggagggg gtgaacgcag ctacgtgcag ggcgagatcg ttggcaaact
     9301 ccttaacttc ttctttttcg aagacggcgg gatcgcccgc tttgatcttc accaatacag
     9361 cgagggagcc ttccccatgg gtgtaggtgg aggccagttc atcagcaccg acatcgtata
     9421 cggtgaggtt ggcgagactc atgttctcct tgatgatgga gatcaggtcg ttcaccattc
     9481 cgttaagctc atcgtttatc tccgtgtagc ccttctcaag cgctacgtcg cagatcttct
     9541 caccgagggc gatgaactcc ttgttcgttg caacgaagtc cgtctcgcag gtgaggttga
     9601 tgagaatggc tttgttcttt gcgaccttga cgaacacccg gccattatcg gttgcccggt
     9661 cttggcgctt cgcaacggcg gcaaggccca ttttctttaa aattttctca gcctcggcaa
     9721 agtcgccgtt tgcttcggtg agcgctttct tgcaatccat cattccggca ccggtggcat
     9781 cccggagttt ctttaccaaa tcagcggtaa ttaccatatg tgctcctcct tagtcgttat
     9841 agagcgtctc gtcctcatca tcgtcttcat cggtgcgatc atcttcctct tcttcctcgt
     9901 cctcaaactt ggaataatcg gcatcgtcat cagtggagta gacaatggct tcgtcgtcat
     9961 cgtcatcgtc ttttttggag acttccggct cttcgatcgg ctcgtcatca tcaccgaggc
    10021 tttcgatgat ttggattccc gcttcgttgt ccgcgtcgat gacggcgttg gcgatgattt
    10081 cgacgaacag gctgatcgcc cggatcgcgt catcgtttcc cggaatcggg taggtgatat
    10141 aggtgggatc gcagttcgtg tcgacgacgg cgatgacggg aatgcccaaa cgggtcgctt
    10201 cggcgatcgc gatcgcttcc ttcttcgtgt cgatcacgaa gatcgctccg ggcagttcct
    10261 tcatatcctt gatgccgcca aggttcttct cgagcttctc tttctgtttc tccaagagcg
    10321 agatctcttt cttggtcaac gcgttgaagg taccgtcatt ttccattcgc tcgatttttt
    10381 tgagtgtggc gatggatttt ttgatcgttg aaaagttcgt gagcattcca ccgagccaac
    10441 gatggttgac atacggcatg ccgcagcgtt tggcttcggt ttcgatcgcc tgctgggcct
    10501 gtttctttgt tccgacaaag aggacgctct ttccttgttt gacgacgcct ctgaccgcgt
    10561 catacgcctc aatgatgcac gctgaggttt tttgtaaatc gatgatgtga ataccgtttc
    10621 gttcgctgaa gatgaaacgg gccattttcg ggttccaccg tttggtctgg tggccgaagt
    10681 gtacgcctga ctcaagcagg cttttcatgg ttactacgga cataatcctc ctattgacgc
    10741 gccaaaagca cgtcaccctt ctcctgttac acaccttgcg gtggagattt ccctaccaat
    10801 ccggcagagc tttagccagt atcccaaaaa tttgattcat tggcaagcct atcaccgtgc
    10861 tttcatcccc ggtggtcgac cgaaccagct ctttgccccg gccttgatag cggtaggagc
    10921 cggcggctcc tacccattca cccgtatcaa ggtaggcttc gatctcatca tcactcaatt
    10981 ccttgaatgt caccgcacat cgatcacagc cctgatgcac atctccattc acccacaacg
    11041 cccacgctga atatacatga tgggtggttc cgctcaagag cctcagctgg gaaaaggcgg
    11101 cttctctggt ttcgggctgc ccaagctcga tctcctcgaa agcgaggagc gtatcactgg
    11161 cgatgacggg atgggttggg gagggccggt ctcgaagaaa cgcctcaagc ttgcgcattg
    11221 ccaataatcg gacgcttttc tcgacgcttt ctctttggtg ctcttcatcc gctttgctgg
    11281 gttggatcaa gacttcaaac cccgcttctt ccagcaagag ttttcttccc agagattttg
    11341 atgccagaat aatggtctta ctcatacagc tcttcctcca atatcatccg ctcctcttcc
    11401 aacgtgagcc acgcggcttc ttcatcagcg aggtttttcc gtaatcgttc ctcttctttc
    11461 accaatttca ggatcttttc ttcaaccgag tagttctcag gttggctcat cagccgttga
    11521 acctcctgaa gctcggtttc aatcacgtcg agagtgagga gagactcttc cgcctggcgg
    11581 cgaagggaga ccagacggtt gtgcttctgt ctctcttcgc ggtaggagcg ggcgggcttt
    11641 ttttcctctt tcgccgcttc tgcgggaatc gcctccttct gctccaggcg atagctgaaa
    11701 taggaccaat cccccgggaa gagctcggga ggattctctt ccgttaaata gagaatgctt
    11761 gtcgctgctt cctgaatgac gtaggcgtcg tgactgacga agatcagggt tccgtcgtac
    11821 tccctgatcg cctgaagaag cgccttcttc gccgcgatgt ccaaatggtt cgtcggttcg
    11881 tccaggatca ggaggttcac cgggtgcatg agcgtcttga ggaggatcag gcgggagcgc
    11941 tcgccgccgc ttaagattcc gacccgcttg aacacatcgt ctccgctgaa gagaaaggag
    12001 ccgaggtatg ttctcagccg gggaatgtcg gcggtcgatg cgacgcttgc aagctcatca
    12061 agcaccgtgt ttgaatcatc gagggcctgc ttcgcgtcct gggcgaaata gccgatccga
    12121 acccccgatc cgacggtcag gcttccctgg taatcggtgt cgatccctgc aaggattctc
    12181 aaaagagtcg atttccccgt tccgttcttt ccgacgacgg cgatccgttc ccctttggtg
    12241 atgatcgagt cgaacccctc gaagacggta agcgaaccgt agcgcttggt gaggttttca
    12301 atcttcaata cctgcttgcc gctgtgcgga gcggggggga aggaaaagga aagatttctc
    12361 aggtgctcgg gaatttcaat gatttccagc ttctccagct gttttattcg cccttggact
    12421 tggcgggctt tggatgcttt cgcccggaac cgctcgatga actccttctg ctttttgatc
    12481 tcctcctgct gttgttcata ggcatggatg aggctttgaa tctcaagctc ccgctgctct
    12541 tcatattggc tgtacgttcc cttatagcgc ttcaattgac cgtgaaagag ttcaaacacc
    12601 tcgttgaccg tctcatccag aaaggagcgg tcgtggctga cgatcatcac cgactcggga
    12661 tagaccctca ggtagtttct cagccagtag cgggcttcaa tgtcgaggta gttggtcggt
    12721 tcgtccaaga ggaggagatg gggtttctcc gccaagactt ttgcaagcgc gatgcgcatc
    12781 tgccagccgc cggaaaactc gctgcaagga cgatgaagat cgtcgtcatg gaacccgagg
    12841 cccttgagaa tgatcgcgat ctccgtcttt cggtcatgat atccctcatc gaggagagtc
    12901 tcctggatct catgaagccg ctgaaggaga taatcggaaa gctcacccgt tgcaagacgc
    12961 tcttcaatcg agtgctgttc atcgacaagc ggaagaaagc gattataggc aagctcgacc
    13021 tgttcaaacg ccgttccatc ctccaggacg atatcgcttt gcggaaggta cgagatccgc
    13081 atctctttcg ggtgggcgca ttcgtagata tccgcgctca ccaatcctgc aatgatttgc
    13141 aagagggtgg atttgccgct gccgttcgcc ccgaccagag cggttcgagc ccgctcggaa
    13201 agcgtaaaag aaatatcatt caacagctgt cgatcggcga atgcaatgga taaccgctgt
    13261 acctgtaagg tagccaaact ccctctccct tggccaagcg ggtgttgatt agccctgctg
    13321 taggcgctat gatggagtct accacacccg cgcttgcggt gacaatccac aggaggtttt
    13381 ccttgctttg cttaacgctc accgcttcga cattggctga aaacaaagca ttggtggaga
    13441 aaaaccgctc atggatcggc gccgccgaac tgcggctcga tcacctcgat gaagatgaat
    13501 tgaaagtggc atcggccttt ccctcgacgg tcgatctccc gctcattctt acctatcgcc
    13561 gagcaagcga tggaggattg tgcaccaaaa acgaaaaaga tcgcctccaa accctctatg
    13621 aggctgcaaa gggggggtgg gcgtatgttg atatcgaaga ggacgtgaaa aaaagcgatc
    13681 tgaagtgtaa ggaccccctc tttgaagggc gggtggatct ggaagaaagc ttacgcgccc
    13741 gaaatatccg gatcatccga tcctatcatg actttgaagg ggtccccgaa gacctgttcg
    13801 gacgggtcaa tcgcctggcg ggcaaggggg atatcccgaa agtggcggta acccctcaat
    13861 cgctgttgga tgtcatcatg ctcttccagg tggaggaaca gcttgcccac atcaaagaga
    13921 agatcatcat cggaatgggg caattgggga ttcccacccg aatcctctac aagcgcctcg
    13981 gctcgatgct gagcttcgtc agtgacaatc aggcagctcc cggtcacctc ggtccgagag
    14041 agatgagcga agtgtatcac gccgatcgga ttgaccgaaa cacccacatc tacggggtca
    14101 tcggcaaccc cgtctatcac acctcatcgc cgaaaatcca caaccgcggc tttgaggcga
    14161 tccggtacaa cgctgtctat gttcccttcc tcgtcgactc cgtccggtcc ttcttcaagc
    14221 tcgccgagct tctgaagatt cgcggttttt ccgtcacgat tccccacaaa caggcggtgg
    14281 ttccctatct gggcaagacc agtcgggaag tcaagcagat cggttcatgc aacaccgtgg
    14341 ttcggatcca ggggatgtgg aaagggatca acaccgacta ctatgggttc ctgactccga
    14401 tccaggaccg gatcgcttcc ggttcgatca aaagcgccct tgtcgtcggc tcgggagggg
    14461 cgagccgggc gatcgtgtat gcgctcatca accatggggt gaaggtgaca attctcaacc
    14521 gtacggtcga acgggcccgg aagctgggcg atgaaacgat gagcggatat gactcgctgg
    14581 aaaacgccca cctctaccaa gggagcgtcg atctggtcgt ccagacgacc agcgtcggga
    14641 tgggggagct ggaaggaacc aatccgatac cttccttcgc gttcaccggc aaagagatcg
    14701 cctatgagct gatctaccgg ccggaagaga cggcgtttct tcaagcggcg aaacgggcgg
    14761 gatgcgaacc ggtattcggc aaggagatgc tgatcgagca gggaaaactt cagtttgaag
    14821 cgttctgcgg gtatcacttc ccccactgga ttcccctgga tctttaaaga aagagcatct
    14881 ttatctggcg gagcaggaag attttggaga gcgggattac ccgggtttcc atctgcggga
    14941 gaatcgctac tttaaaaagt gcttcctttt tgggtgtata ggtcagctcc agcgccaagg
    15001 ccatgagttc ctcgcccccc atgaagagct ggacgacggt tccttccttc gaggaggctt
    15061 gtttcgcaag ggagattttt ccctgataat caaaaccgcc cgcttcaatc cttgtataga
    15121 ggatctgggg ggtgatttgg gatttgttga tgatcaagcg ctcttcaaag cgctccagga
    15181 gatcctcctc cacttctttg ggatagttct tcctgatgat cgcctctctg agcttctcat
    15241 ccggtacaac gggaggaagc gttaccgatt ttttgaactt ctttggcttt tgaaactcct
    15301 tcggccattg aagggcgggg agctcccggg tttccccatc gcttccatgg gcaagttcgc
    15361 ctttggttga accgagactc aagcccgcct gggtcaggat ctgccgccag attcgctcag
    15421 aggagcgctt catcaggaag attgtgggcg aaagacggtc gaggatgtgt tcttgaagag
    15481 ccggcagctg ttcgacgatc cggccgaccc gttcatcggt gtgcaggaca aggccgtcat
    15541 agatctgaac ctgcctgctc cgttcggcga tcagatcaag ggatttgagc agcgccgtgt
    15601 tcagcgttcc gttcacgtat gttttcaagt atgacctgat ctcatcatag ccgacccctc
    15661 cgtccaaggc gctgcgcagt cgttcttcgg tgattcgcca caatcgggtg cgatcgaggg
    15721 tttccaaggc ggcaaaacga acgagaagcg agtcggcgtc cagtgacccg aggtaagaga
    15781 ccgaatagtc gctgtccacg atgaaggagg aggccggctc cccttgggtg taggagacat
    15841 gattgtatcc gcctttcgtt ttgaccaaca gtccaagacc gagcaacacc gaggtaaggt
    15901 cgcaacgctt gacatggtgc ttgaaggcga gggcttggct caggcgcttc aggccggaag
    15961 cggaaaacgt tcctatatcc tccaaaagaa agaccaaatc agcacaacag gtgttcaggg
    16021 cgccctccat atgcccggcg agcatcatcg ccttgatctg ctgttcatcc agaagaaaga
    16081 ggtcgtgagc ccgctgccaa tcgacccggc gctgagggtc ttcgatgatg attccgagct
    16141 tgacgaaact gctgtggatc gtttcaaaga tggagggaag atcatcgctt gccatcgtcg
    16201 gaaagagcgt ttcatactct tttcggaaga aggagcggcc cggtcgttcg gcaagggtga
    16261 ggatcgcctg aaggaattcg cgatagctga aggggtgggt atgaaccgtg ccctcttcaa
    16321 ctccgaaaag gggtgtcaaa ccggcaaatc ggtagaagtg ctcttccaaa agcggattgg
    16381 gaaccagcgt attgcctgtc gctatgatga tcatgcgttc gcgcagattc gcgacccgaa
    16441 ggagcagttt gccatatgga tggattccct ccagaagggt cgccagctgc tcaaagcgca
    16501 agcctccggt gaagagcagg gcggtgatga tcatttgatc gaaggaatcg atgagggaaa
    16561 gcatcttctt ccggttctct tcaagcgcaa agaaggcgat cagtcgagaa gagatatgct
    16621 ccttgtgaaa cggcgactcg acgaggccaa gatagtttcg ggccaaaaag aagtattcat
    16681 catcggtata ctgctcaagg agtgtcttca gttgttcatg actcattccc acacctcgat
    16741 ttcatagctg tagccctgtt cggcaagaaa cttttgacga tttgcgctga actcttcctc
    16801 actggagtag cgagtgatca ccgaatagaa gaaagacgat cggcctttgg gccggagaat
    16861 tcgcccgagg cgctgcgcct cttcgcttcg ggagccgaac gttccggaga tctgaatggc
    16921 gaccgaggcg tcgggcagat cgatcgcgta gttggcgacc ttgctgacga cgaggattcg
    16981 gatctccccg tttctaaagg cattatacag ctcgtcccgc tttgcattgg gagtgcttcc
    17041 cgtgatgatc ggaagatcga agagctttgc cacctcctca agctggtcga tgtactggcc
    17101 gatgatcagg ataaagtcat cactgtggcg ttcgatgagc gagcggacca cctcgatctt
    17161 caacgggttt tcactggcga ttcgatattt ttgacgcttg gtggccaagg cgtattctag
    17221 ctccagctct ttcggcagat ccaagcgaat ctcgtggcag tacgcctggg cgatgaaccc
    17281 ctgctgctgg agttcgatcc acggaacgtc gtatcgcttc ggtccgacca agctgaagac
    17341 ttcatcctcc cgcccgtctt cccggaccaa ggtggcggtg aagccgaccc ggaacgtcgc
    17401 ctgcaattcc gccgtcactt tgaaaacggg cgcggggagc atatggactt catcgtagat
    17461 gatgagaccc caccgtcctt tggtcaggag atccatgtga gcaaacgggg cggttttgct
    17521 cttgcggtag gtaaggacct gataggtgca caccgttatc ggtttccgtt ctttcagatt
    17581 gccggtatat tcaccgacat cttcgctttc gagcgtggtc ttgtcgatga tttccctcat
    17641 ccactggtgg accgccacca cgttggtggt tacgatcagg gtgctggttt tcaagcggct
    17701 catgatttcc aagccgacga tcgtttttcc cgatccgcag ggcaggacga tcgtaccgaa
    17761 gccgcggccc cggccaagat ctcccaaaag ggcatcagcc gcttggctct ggtacggtcg
    17821 gacgatgaac ggctgtccgc caagggtcgt cttgcgtttg tcaaacatga gaggaggacc
    17881 tttctcaaga ggaatccggt catccaccgg gtagccgagc ttgatgagac gaagcttgag
    17941 ctcgccgcgc aggtaccgtt caacgaggaa agagtcctcg tccttgacga cgagaatctt
    18001 catcagctcc ttgcggtgtt tgatttccag tcggattttt tcaattgaaa tcgttaaaag
    18061 ataataggct tcatcagtgc tttcggtcag aaccagagac ccccatcggc cggcgatgtc
    18121 ccgaacgaaa aagagtacgc tttccggcgg aggaaacttt gaccactttt taatcatttc
    18181 aacgatcgct tccccgtcaa gcccctcacc ggaggcgttc cacaggctga tctcgctgat
    18241 tcgataggta tgcatatgct cgggagattt caccaattcg caaaaacgga tcagatcttt
    18301 ccgacacgct tcgctgtccg ggtggtggac atccagaagg aggctgcggt cgctttggac
    18361 gatcagggga gtatctttca tgcatctttc ccgaacgccg tgtaatagag gtcgaggagg
    18421 gtgatggcgg tcatcgcttc gatgacgacg accgctcgac cgcagatgca cgggtcgtgg
    18481 cggccggtca cggtgagcgt cacatcgttt ccctccttgt caactgtccg ctgcccgata
    18541 ccgatcgagc tggtcggctt gatcgcagcc tggaagacaa tgtcttgacc gctggagatt
    18601 ccccccagaa tgccgcctga gtggttgctt aaaaatccgt ctttggtccg ctggtcattc
    18661 gcctgactgc cgcgcatcgc cgacagggcg aatccggatc cgaattgaat tcccttcacc
    18721 gctccgattg aaaggatcgc gtgggcgagg agcgcatcca gttttccgaa caccggttct
    18781 ccgagaccga cgggagctcc ccgaacgatg cactcgatga ccccgccgat cgagtcgttc
    18841 tcctttcgaa ccgtttcaat cagccgaacc atcgaagccg ccgcctctcg atcggggcag
    18901 gcaaggatcg ggtcgggatc ttcccaatcg cgcttcattg cgacaatatc gccgacttgg
    18961 acgaccccgg cccggatcgt gatgccccgc ttggccaaaa gctgtttcgc aagtgccccg
    19021 gcggcgaccc gggcactggt ttcccgcccg ctggatcggc cgcctccgcg gatgtcccga
    19081 accccgtatt tcttgtaaaa ggtccagtcg gcatgaccgg ggcggaacac atccttgagg
    19141 tgggagtagt cctttgggcg ctgattggtc gactcgagga tcatcgcgat cggagttccc
    19201 gtggtgacac cttcatacaa gccgctgagg atgaccagct tgtcgatctc gtttcgttcc
    19261 gtcccgctgg gatttccccc cggacggcgg cggtccatct ctttttgaat cgcttcaagg
    19321 tcgacggtga agccgctttc gaccccgtcg acgatgacgc cgagagccgg tccgtgcgac
    19381 tcgccgaagg ttgtcacgga aaaatggttg ccgaatgtgt tgtgacccac gtctactcct
    19441 tcaagccaag ggaagaaaga accaattcaa cattctcctc cttcgcttgg tcaaagggta
    19501 attgtatcaa atggttgcaa tccttggaat agcgtcgatt gcgccgctcg tagagccgat
    19561 gaaacgaccc ttcaacgtcc tcctcgtcca gaaaaggggg aattcctccc cggagaattc
    19621 gctctaaaag atgtgcttcc tcgagataca aatacaccag cgttccccgt tccttaacca
    19681 tcttcatcaa gggatcgttg tcggcggctc cgccgccaag gctgatgacg aggttctctt
    19741 caccgatgat cgcctccagc acctcgacct ccaactgctg gaaggcttct tgaccgaagg
    19801 cgcggtaaaa cgttcgaatc ggctcaccga tcttccgttc aatctcccgg tcaaggtcat
    19861 accaccggcg gcccagccgc ttggcaagca gctttccgat cgtggttttt ccgctgtgct
    19921 tgattccaca gagaaagatg tggctcatct caatcctcga acagctcgac ccaggctagc
    19981 gggtgggtgt tggtccgggg aactacggag cggtttccgg caaagcgttc tttcctgctt
    20041 ttttcccggt ccgccaattg gcggctttca atcttcgcct gatagcgggc ttcatggagg
    20101 ttttttctca tatccgcttc atagcgattg attcggattg ttttcttcaa cgcgtaatga
    20161 aaggtgatga tgatcaagta ccaaagcagg gaaagaccga gcggctgata ggtgaggatg
    20221 ccgccggtgg aaatgaacga gttcgggtcg ctggcgaccg tgtagagggt aagcgcgttc
    20281 ggaacgatca aaaaaagggt gagaaggagg gggatgaacc aatacagccc ttggcgtgaa
    20341 aacaggaagc gcaggcacga tccgaaggcg agaaagttca gtaaaagcgt caacaacggc
    20401 aacacataga ttgcaaccat ggatcctccg ctggaatagg ttactttttc catgatacca
    20461 taatcatcgg atgttgggga atggtatgaa gttgcacaat gagatcgatc ttgaactttt
    20521 gatacaccga aatcggccgc gggagatcga tgtccgcggt ccgttttatc gggatacgac
    20581 agcgatcatc cactcgtcgg cgtttcgtcg cctcaagcac aagacccagg tgttcttcgc
    20641 cccctcgaac gaccacatct gcacccggat ggagcactgc ctccacgtcg cctcgatcgc
    20701 atcgacgatc tgcaaggggc tcgggctcga cagcgacctg gcatgggcga tcgggatggg
    20761 gcacgacttg gggcatacgc ccttcggaca caccggggag aagatcctct ccaggatgat
    20821 ggaacaacac ggccttggca acttcgagca tgagaagcac tcccttcggg tagtcgattt
    20881 tctcagtgag cagggaaggg gattgaacct cacctatgcg gtgcgggacg ggatcgcctt
    20941 tcacaacggg gaacacctcg ttaaatcgat tcggcccacc tttgaggtgc gggatctcac
    21001 ccgggacctc aggaacctga cgattcttcc cgccacctat gaaggggcgg tggtccgctt
    21061 cagcgatacg atcgcctacc tcggacggga ctatgaggat gcgtgccggt tgggcgtcat
    21121 cgacaaggaa gcgctgcctc cgagagtggc gacgaggctg ggcaaaacaa acgcccggat
    21181 catcgatacg cttgtcaacg acctgattct gcattccaac cctgttgagg ggatctcctt
    21241 cagcgatgag ctctttccgg tcatcgagga gatgatggag ttcaactacc gggaaatcta
    21301 taaaagcccg atgctggagg ggtatgagcg gtacttcgaa cgtctcttca ccctgatcat
    21361 ctcctatttg gaaactcttc tttcggagtg cggcgatgat gaacgatgtt ataaggatga
    21421 gcggaacatg ctggcgatgg ggttctacca ccacaaggag gagatgcagc aggcgtatct
    21481 ggcccatgac ggaaatccgg accgcctgat ctacgactac atcgccggaa tgagcgacca
    21541 cttcgccctt gattgcgcca atgagattct caagccggag cacctcaacg attcgatcga
    21601 gcagtcgctg accggcaagt ggttcgatgt ccgttaattc agttccttgt ccagctgctt
    21661 gatttcaccg atgaactgtt cgatgtcgtt gaacgccttg tacaccgcag caaagcggac
    21721 gtacgccacc agatcgacgg tcttcaattg cttaagcgct tcctcgccga tctcggtgga
    21781 ggagatctcc cgccggtttc cgagcttgag catgatcgcg tcttcgatgt tttgaaggag
    21841 ggtctcgatc tggtgttcgc tgatcgagag cttctccgtg caggagcgga ttccgcgccc
    21901 gactttggag atgtcgaaca tctccctccg gccgtcccgc ttgatcacca tcaccggctt
    21961 gtcttcgatc cgctcatagc tggtggatcg atacccgcag gagaggcact cccgtcgcct
    22021 ccggatcgcc gttccgctgg cgttctgtcg tgattcaagg actttgtctt ccaatgagga
    22081 acaacgggga caccgcatct ttgcctcctt atcaccccca tagtacaaag ggcgggatga
    22141 aaaggcaatt gctagtggtg tatcacttcc aaatttcccg gtattttaac aaaacgccac
    22201 tagcgaataa ccgactcgga acatttgtta gtttggagaa aaatacctga agttttgttg
    22261 taattagcta agccttgtga taataataac agaaggagga tgcaatggcc gagtcggtat
    22321 ccagttgtgt taaacgtatc gtcgataagt ctcccttcat cggagagatg ctcaccttgg
    22381 ggatcctgtc tttttccaac tatgccgcgc tcatcagcga tgaagtcgaa cgagccctcg
    22441 gcaagccggc taaaagcagt gcgatcgtca tggcgcttcg gcgctatgga gaggacctga
    22501 agcgctccaa tctggtccgt gacacttcca atgtcaatta ccagatcgtc atgaagacga
    22561 acatcttcga catcaacctc gttcgccgcg attccttcat cggaaagctc gggtccctgt
    22621 acaaccagat ctccaccgag aaaggggact ttctcaacat cactctgggc agtcatgaaa
    22681 tctcccttgc ggtgagtgaa aaataccgac ccctcatcaa ggagcttacc aaagacgaag
    22741 aggtgctcaa caaggaagat gatcttgtcg cgctctccct ggtgttcacc ggagactttc
    22801 tgcagacgcc gggaatcgtc tatgaggcgg tccggcatct tgcctggcag cagatcaatg
    22861 tcatcgagat cgtctcgacg atgaatgaac tgacgttcgt catctcccgg gaggattcga
    22921 tgcgcgcctt cgacgtcctg cagaacttcc tccgtgttca ataagctgac cctgatcgta
    22981 tccccgatcg gaggtggaaa gacgcggtat ctttccaacc tgaaccttcc ttccccccat
    23041 tgcggggtgg tcacatcttc caacatcgag aagacgatct atcacatcaa ggacctcgcc
    23101 ggcggagaag agcgattgct cctcagtgaa gcatacctgc ccaacgctcg aagattcggc
    23161 cgcttcttcg tccttgaaga aaccttcgac tgggcgaacg aacggatcat ctccaacctc
    23221 gaagcgtcca aagccgtcgt cttcgatgag atcggacgga tcgagatcga ggggtggggg
    23281 ctgaaatctt cctttctcaa ggctcttcgg acgccggcga tcgagatcta cgccgccgtc
    23341 cgcgacactc tcctcgatca agtactcaag acatttgact tggaagaaag
//
LOCUS       DAOSLI010000028        23077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2460_length_23077_cov_26.447224, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000028 DAOSLI010000000
VERSION     DAOSLI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2460_length_23077_cov_26.447224"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..608)
                     /locus_tag="PLF67_05500"
     CDS             complement(75..608)
                     /locus_tag="PLF67_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89503.1"
                     /translation="MKKFLATLLIIALALTGVFAATEATLTLETDITPVNLIKLYAVG
                     DNSAGVASGVKYTLPAEFSTISLSQIADLEFTTNNAKQVTVSMSGTEFKSTENNVIPY
                     TLTYDKDGVDKTITPVDGQSGLSGHETVLYQANSVESLMTIRKDLSLTIGDGSTSYAN
                     YPAGSYEATITFTIAAL"
     gene            complement(1107..1631)
                     /locus_tag="PLF67_05505"
     CDS             complement(1107..1631)
                     /locus_tag="PLF67_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HOE89504.1"
                     /translation="MSRETIYLIDTNSLITPKNSYYPFDIAPEFWKSMAEKIEEGSIA
                     ILDLVKKEILKPAKKDDLANWMESLTIKRYINHGKQDIVNKYAEILQFIQQDSCYTEK
                     ALRQWTDGSTADPWLIAAAVVHGFTIVTFEKAVHINANNPSGKAKIPNIAQQFQVKTT
                     DLFGMIRDLGIRLC"
     gene            complement(1631..2773)
                     /locus_tag="PLF67_05510"
     CDS             complement(1631..2773)
                     /locus_tag="PLF67_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOE89505.1"
                     /translation="MKATYAVSPEVVEWVISQVGEHTEPLKSLNQWKTGEKIPTFNEI
                     QDISKKTRIPLGYFFLKTPPKEEIKLLEYRTIASEEFDNPSRDLIDTIADMEEIVDWT
                     RDYLIAEGSEINPIVGKLKHETNVMVMAEYIREVLGLSIDWFKTTANSFNYLRDTISE
                     AGVIVMMNGVVRNNTHRPLDINEFRAFTLIDEFAPLIFINAADSASGRLFSLLHEFVH
                     ICLGVDNLFNDNRYSTFNGVKKQETLCNAVAAEILVPASLFGSIWDAHSDQSGSIDEI
                     VENVARYFTCGRIVIARRALDTGRIEQPAYDEIVRIARADFHKQKQTKKTGGDFYRTK
                     ESRIDKRFLAFLVDSVSRGKTLYSEAFKLTDTNRHTFPKLVERMEF"
     gene            3062..3991
                     /locus_tag="PLF67_05515"
     CDS             3062..3991
                     /locus_tag="PLF67_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE89506.1"
                     /translation="MYIYDGLNKKLKERNLKKSDLTSVLSISSRTLAKISKGEKLADS
                     VMSKLCTYFSCSKDDLCRIVSDNPILQTLREEKEHKIPGGLYHELQIRMTYNSNHIEG
                     GTLSEEQTRRIFETNTLDAEDGLPVDDIIETVNHFRAIDYCIDMAEEPLNEEIIKHLH
                     YLLKSRTKNETLFWFRVGDYKLRPNVVGGIETTAPKDVATDIQALLSAYLKKQTVTLK
                     DIIDFHYRFERIHPFQDGNGRVGRLIAFKECLKNNLMPFIIEDRKKQYYYRGLAEYTN
                     EPGFLVDTCYDGQDTFRALVQRFDIDVSALDEK"
     gene            complement(4179..4250)
                     /locus_tag="PLF67_05520"
     tRNA            complement(4179..4250)
                     /locus_tag="PLF67_05520"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4216..4218),aa:Cys,seq:gca)
     gene            complement(4470..5360)
                     /locus_tag="PLF67_05525"
     CDS             complement(4470..5360)
                     /locus_tag="PLF67_05525"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOE89507.1"
                     /translation="MLFNNNEYFELVEQVKQEILTAQHKASLSLNQGLVLMYYRIGQH
                     INEHKEWGNKFIDNLAKDIRISFPRAKGYSVRNLKYMAKFAQEYPDEQFVQTVSAQIP
                     WSHNIAILNKVNDLDERIWYIKKTVENGWSHAVLVHQIESKLYERQALAEKVSNFEAR
                     RMAPHSELAVQTMKDPYIFDFIPFKEQMVERAIEEALVKDITKLLLELGTGFAFLGHQ
                     YQLNVGGDDFYIDLLFYNLNLRSYVVIELKTGEFKPEYAGQLNFYLSAVDGTLKRTEI
                     IHQLDFCYVKVRITWLQNTH"
     gene            5583..5852
                     /locus_tag="PLF67_05530"
     CDS             5583..5852
                     /locus_tag="PLF67_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOE89508.1"
                     /translation="MQPVEKTRTVKVPISVSFDSTLVEQIDSICKERGCTRSWFVNKA
                     VEEYMLEYVEDRDDYETAVAALEEFEKGDRKTYTLEEVYAEADLL"
     gene            5849..6109
                     /locus_tag="PLF67_05535"
     CDS             5849..6109
                     /locus_tag="PLF67_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE89509.1"
                     /translation="MNITFSDKAKKQFVKLDKPVQNQMRKFLDSLKNLQNPRTRGKEL
                     VGNRRGIWRYRVGDYRILCQIKDKELCIFVVEVGHRKEVYDR"
     gene            complement(6240..7133)
                     /locus_tag="PLF67_05540"
     CDS             complement(6240..7133)
                     /locus_tag="PLF67_05540"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOE89510.1"
                     /translation="MADDKVPVLVEKREIQDMIYTIRGRQVMIDSNLADLYQVTTKRL
                     NEQVNRNKNRFPPQFMFQLTEDEYRDLRSQIATSNDGDKHGGRRYMPYVFTEQGIAML
                     SSVLRSNVAVEVSIRIMDSFVEMRRFLVSNQELFSRLDRVELKQIETDKKLEQVFNYI
                     ATRTEVKQHIFFDGQIYDAFSFIVDLIKKAQNKLVLIDNYVDTNTLNLLCKKNNGVSV
                     LIAGSGKGSLTSKDISKFNAQYQGLLVKTRNDFHDRFLIIDDTEVYHIGASIKDAGKK
                     SFGITKIEDQTLLDSIINKVK"
     gene            7419..8210
                     /locus_tag="PLF67_05545"
     CDS             7419..8210
                     /locus_tag="PLF67_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOE89511.1"
                     /translation="MANIPASRQQYILSRIQDEGTVNVNQLSDALDVSELTIRRDLDQ
                     LAKRGLVERTHGGASLRRNLPVEPDYLQKASEFPLEKSMIGQGVLDLVEDGDTLYINS
                     GTTTLEAIRALVASGKRLTIVTNNIDAAYVCRESQAIRLVLAGGVYRTRSHSVSGSLS
                     SLVVEQIWANKAIIGVDGFSVQAGLTTPVLEEAETTRAMIKHTAGKVIVVAAANKIGV
                     VSNYKTVSLEDVDILVTDEKGAELVSQMEISDDLSIVVAPTRRSE"
     gene            8207..9349
                     /locus_tag="PLF67_05550"
     CDS             8207..9349
                     /locus_tag="PLF67_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOE89512.1"
                     /translation="MKYLDEYAKQYEDCAWLPFEELEAFMKEALIHAGVPREDAEIIS
                     DVLIESDKRGIDSHGIGRLKPIYIDRIDDGIMLPTTKIEVLRDKAAAAVLDGHNGMGH
                     VVSHKAMSMAIEKARTYGIGMVTVRNSNHYGISGYYATMATRKGLLGITGTNARPSIA
                     PTGGVENMLGTNPLVFGMPTDEEFPFVLDCATSVSQRGKIEVYGRAGKELPEGWVIDN
                     EGRSRTDTLQVLKDLTTGKAALTPLGGIGEVTAGYKGYGYATVVEILSAALQAGSFLK
                     ALNGFDENGNQIPYPLGHFFIAIDPEFFMGLETFKKITGQILRDLRASKKAPGEDYIF
                     TAGEKEYLAYQYRKEHGCPVPPSLQKVMITLRDRWNMDMSWDFEKK"
     gene            9363..10655
                     /locus_tag="PLF67_05555"
     CDS             9363..10655
                     /locus_tag="PLF67_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOE89513.1"
                     /translation="MNQDLLKEKALAYHIQDGVPGKVSVVPSKPCQTAEDLSLAYTPG
                     VAKPVLEIAANPEDAYKYTSKGNLVAVISNGTAILGLGDRGALASKPVMEGKGVLFKR
                     FADIDVFDIEIDETDPKKLIEIIKKISVTFGGINLEDIKSPECFEIEQTLIEECDIPV
                     FHDDQHGTAIIATAGLMNACEVVDKKIEEVKVVINGAGAAGISCAKMFLNAGVKRQNL
                     ILCDRHGVLRRDRLEGLSPEKQDFAIDTELETLADALKGADVLIGLSVADVVTPEMLL
                     SMDRDPIVFAMANPNPEIAYELAMATRSDLVMATGRSDYPNQINNVLGFPFIFRGAMD
                     VRSRIISEGMKMAAAKALADLTKEPVPDSVAKAYGGKKFTFGREYIVPKPFDPRVIEW
                     EAVAVAKAACEEGLAAAPITDWDAYRESLKQRMAHWWA"
     gene            complement(10711..11316)
                     /locus_tag="PLF67_05560"
     CDS             complement(10711..11316)
                     /locus_tag="PLF67_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89514.1"
                     /translation="MAIIKSAWEIAMERANAIQADPKKIQKDLTIKEGRQIAAAFLLD
                     IDGTKEEAEKKYDSYSNEAKAHVKEGMALTLLSNLTLPKTPNYAETIPKLIALGAMIS
                     DDDEELVELLAQMQGFFTQYVETQEDLIERLKEQFAPHLQQKEAQLRAQYGPNFVLRP
                     EQDPEFMKVLDKQLKTLEEQYTNVLDNAKERMKEILGIEGQ"
     gene            11426..12622
                     /locus_tag="PLF67_05565"
     CDS             11426..12622
                     /locus_tag="PLF67_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOE89515.1"
                     /translation="MNNHMKKHSYPRYYDEPYRTTLETRVVSVEGSKVVLEETIFYPE
                     GGGQGGDWGTVNDCPVLDTVSGDDEEIIHLVRDPAFKAGDRVVLTLDWNRRFHFMKNH
                     TAQHAASGILFKHFGIGTVSVHLGTEVMMIEVDRQEVPDSVCLELQSLVNRAIVEGHP
                     VTSETKSHEDAEALGLRRPIKVEGDVRLVHVDGVDTIACGGLHVSNTREIGLFLYEGQ
                     EKIRSHARLIFTVGEVAWNRVLLNRTIVDQLGTLHSSPPEALVETAAKACEAAASEKA
                     FLGRLRNRLASVLLDLKLAGETDPLPVITWEVEDEITLKDLAQAASRVANLALCATLR
                     LEDGKLAWLIVLTGEGAAKLDIAKHRSDLLSSINGKGGGKAPIFQGVGEGDPAELFAR
                     FRDLLR"
     gene            12619..13251
                     /locus_tag="PLF67_05570"
     CDS             12619..13251
                     /locus_tag="PLF67_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89516.1"
                     /translation="MSEKQQTEPIEIDELHRIDPKDGELSFRELFKKSIWAIVVLMAV
                     TFTSLFLFKKLGWADAEVLHDFIDAFGAWAVAGYIFVMELTMFPVSVDMVWPFVLTWS
                     VPKAVIVMGTASVVGSFCAYLVGRLLGHIPIINNYVRRNTKPETAAMMKRYGAWGVAI
                     GGIAPLPFSTICTLAGMAKLPALRVLLALQIRYVRMGLYFLIFSGFLSLG"
     gene            complement(13238..14644)
                     /gene="purB"
                     /locus_tag="PLF67_05575"
     CDS             complement(13238..14644)
                     /gene="purB"
                     /locus_tag="PLF67_05575"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOE89517.1"
                     /translation="MQEFNHQTFISPFTWRYGSEAMRRIFSEVHKRTLLRTIWIALAR
                     AQMRVGLVTEEQLAELEATKDRIDIARATQIESEIHHDLMAEIHTWAEQCPNGGKIIH
                     LGATSMDVLDNMDALRLTEALDLTISKTRELLILFKEKMEAYRELPTMAFTHIQPAEP
                     STVGYRFAQSAQDLKEDLEELIRVRSSIRGKGMKGAVGTAASYAQLLEGTGLTAIELE
                     EAVMEEIGLAAYTASTQIYTRKQDLRVAQALSGLAATLSKFFFDFRILMSPPIGEWSE
                     YFASKQVGSSAMPFKRNPINSEKINSLCRLVSTHVDLFWQNAATTVLERSLDDSANRR
                     IAIPEIFLAVDEILVTAIRTVGRMTVHEEAVGRNLNAYGLFAASERLLMELGRRGADR
                     QAMHELIRNHSLKAWEAVRSGKENPLAKSLSKDKKVLAFVTEAEVLEFMKVEAYIGDA
                     VLRTTMVAKEIDAFLNRD"
     gene            complement(14676..15737)
                     /locus_tag="PLF67_05580"
     CDS             complement(14676..15737)
                     /locus_tag="PLF67_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89518.1"
                     /translation="MFKPATKLMIALAVTVFLLTGCAKESANENGNQKLYVYNWSYYT
                     PDSVIEAFEKEYGVDVVLDYFASNEEMFAKLRASSQESGYDVIFPSADYVSIMMKLGM
                     LEKLDLSKIPSSRYITEFALSKATYDPKMEYSVPYYLGASGIAVHTKYASNYEKSWNI
                     FGDKRFRDRMVMMDDMREVLGDALVHLGYSVNTTNPAELERARKLVNDQWKPNLVKFD
                     AESFAKGFASGEYYVSHGYAEAIFEEIPEEKWAEVDFFLPEEGGPLYMDSLCIPKGAP
                     HYELALAFIDFIHRPENYAQLLDRFHFPASVNIEADRYRTTTPFYTVEQLANYELKED
                     LGANLEMYNKAWETIRYVD"
     gene            16064..17215
                     /locus_tag="PLF67_05585"
     CDS             16064..17215
                     /locus_tag="PLF67_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89519.1"
                     /translation="MSEFKKDEKRAAPFLEMKKVSKIYAPSRQHHIKAVNDLSFTIDK
                     GEFVTLVGPSGCGKSTTLRMIAGFLRPTSGDILIEGKSITALGPEMRNIPMVFQNYAL
                     FPHMTVFENIAYGLEAKNLPFDAVAHDVAMICQMLNLVGTETRHPNELSDGQQQRVAL
                     ARALVLKPKIMLFDEPLSNLDPRLRIQTRFEIKRMQQLLGITVLYVTHDQAEALSIPD
                     RILVMNRGTIVQEGKPRALYDRPGTPFVADFISNANFFDGFVLSASAETIQLSIQEME
                     FTVSRDRCSIFVEQDDSLLVAINPISVGMSPFEGECGFNQCVGIVRSSLFVGQSFEYR
                     VSFGDETIRVLHPNLLGGVREFEEGTPVILTFYPESFNLYGREVDHHER"
     gene            17205..18188
                     /locus_tag="PLF67_05590"
     CDS             17205..18188
                     /locus_tag="PLF67_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE89520.1"
                     /translation="MNARLVGRQILELVLASLGTCLLLSVSKTLVVGCTVLYLALRPA
                     NRWQIRLVRTLLLWGVYALVSSSRALDVLTVYAGASIVAAWTDRRIRWNRKHQTQPGY
                     EQLLSTLAHEIRTPLTIMQTTQSVLMEQSAGKLNEQQQKFMESIYINTQRLITFSEQM
                     LTLIKLGKDWEPDMTKSVDLKLLVRQVIETLQPQLSIRKQEVSLQFPALVSHPRADEA
                     WIRQVLINLIHNASKNTKEGGLIIVSVTQDDRQIVTTVTDNGQGIELAGREALFQEFF
                     QERKDSQQDGFGLGLSIVRSIIAKHQGKVYITSTPNQGTMVSFSLRVGAPW"
     gene            18185..18868
                     /locus_tag="PLF67_05595"
     CDS             18185..18868
                     /locus_tag="PLF67_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE89521.1"
                     /translation="MKNRILVVDDEQAIVELLHFILTNAGYEVASTGRGDEVAPLARE
                     FHPDLVILDIGLPGLSGLEVVPLLVELDTPVLVLSSHDRDDQIIEGLEVGAEDYVSKP
                     FNHKELLLRIEKIIRRTKVEQIIQIGSIMVDIRRRVLEIDGQEITLTPTEFRIIEYLA
                     LHAHVPVSAEELLKQVWNAEEWIQGPEMVKVNISRLRKKIEPNPSTPRFLVNRWGFGY
                     LLTDTQEVS"
     gene            18968..19993
                     /locus_tag="PLF67_05600"
     CDS             18968..19993
                     /locus_tag="PLF67_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89522.1"
                     /translation="MKSMKKALIVLSMIGLLLAPVFAAGVQEKASTTLNVYTIMPEKY
                     ATTVFEEFTKDTGIQVNFVRLSSGEALARLIAEKANPQVDALWGGPADTYEAGVLEGV
                     FEVYVPSEASAIPAQFKSSKGYWTGIGVIPLTFLSSNSFLKEKGLQPPTSWNDLLDPR
                     YYNALQMADVRTSGTATERVYSLIQVWGEDKAYEYQKQLHKNIQLYTKSGAGGAMPIA
                     TGQAGSGIFYIVDALDIQQQGYDLVISYPVEGVTYGVEGSGIVKGAKNLDAAKKLMDW
                     ASSKRLGQVMVDNKINYIPTRPDVVVTNPALDMSKVKLLETSVAWKGANRERLVQRWI
                     DEVIQGQ"
     gene            20062..21732
                     /locus_tag="PLF67_05605"
     CDS             20062..21732
                     /locus_tag="PLF67_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE89523.1"
                     /translation="MKRDPALFMALVTVWLSFAVFIFLPFLRLLGATFVVDGKLSLAN
                     VKTVLSKAYNVKALYGSLVLATAVSITGTVVGYLFALAVTRTRISKVTKGIIGAVAML
                     PLISPPFTSSISLTLALGPNGMLLKALGIGDFSIYGFLGTWISETLTYFPVAFMVITA
                     VLNTMSATLDDAALSLGASRWRMFRTLTIPLSLPAIVNSILLLFGASLADFATPLILG
                     GHAFPVLPTEAYLLITGMFDLKGGATMSFLLLVPALLVFFLQRMMLGKKSFVSVTGKS
                     GSRTEFARLPQSLNVVIAVVCLIVVAFIIYLYAIILWGSLVKVWSVNNTPTLEHYRYV
                     FKHGQKAIKDTVLIAIISTVIGSLLAVVIGFMIQRKEFVGRRLLEFSSMLNYALPGTV
                     VGIAYIIAFNTKPLVLTGTLSILVAAYVFRYYATGIRSVIASLEQIDPALEEASLSLG
                     AGTVRTFTHITVPLIIPALISGMRYLFIHCMTAISATIFLVSVRWTLLTTRILESMTE
                     LQFSQASAFSIVLIAIVFLADFILKHVLKFTTAHITRSQEISYEQIGA"
     gene            21713..22828
                     /locus_tag="PLF67_05610"
     CDS             21713..22828
                     /locus_tag="PLF67_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89524.1"
                     /translation="MNKSALELIGVSKLFQKDGESVLAVDAVGLTVKEGELVTFLGPS
                     GCGKTTTLRMVAGFELPTSGRITIKGKDMTNVPVNKRGIGFMFQTYALFPHMSVYNNV
                     AYGLKARGEKDREIKSKVREALRLVGLEKTEHRFPNQLSGGEQQRVALARVIVMEPSL
                     LLMDEPLSNLDAKLRIHMRTEIRKIQQDLGITCLYVTHDQSEALTMADRIVIMNKGRI
                     EQIGTPFEIYSNPQSVFVADFIGQANIIRLKITAVGSDTISVQIPRGGVFTARALRPE
                     RLKKQEAIYKVGAEASLIIRPEQIQLGPAPAKEDQFPAIVESAMFVGSHIEYFVKLPN
                     DSVIKALVPYQTGMHIFSAGDDVELSFDVASAVFLPN"
BASE COUNT         5341 a         5862 c         6157 g         5717 t
ORIGIN      
        1 aagatttaga catgaaaagg tcttgagtac aaaaaaagga cctccgaaga ggtccctgat
       61 gatcaagatg tggattagag ggcagcgatg gtgaaggtga tcgtggcttc gtatgagccg
      121 gccggatagt ttgcgtagct cgtgctgcca tctccgatcg tgagggacag atcctttctt
      181 atggtcatga ggctctctac ggagtttgct tgatacagga ctgtttcatg accggacaat
      241 ccgctttggc catcaaccgg tgtaattgtt ttatcaactc catccttatc atacgtcagg
      301 gtatagggga tgacgttgtt ttcggtgctc ttgaactcgg ttccggacat cgagacggta
      361 acctgtttcg cattgttcgt ggtgaactcc aggtcggcga tctgtgagag actgattgta
      421 ctgaactcgg cagggagcgt atacttcact cctgatgcta ctcctgcaga attatctcca
      481 acggcataaa gcttgatcag gttgaccggg gtgatatcgg tctccagcgt cagagttgct
      541 tcagtggctg caaacactcc ggtcagtgcg agtgcgatga tcagaagggt agctaaaaac
      601 tttttcattg tattcgtcct ttgagaagct ttcggcttct gtgcaagttg tagccttttc
      661 ccaggctcgt ttgtaggata ctgattatcg aatcttgtgt caatagtttt agagaaaata
      721 tgtaaatatt tttaattaat aaagaaaaaa cattataatt atatatataa tataatgtta
      781 atattcatat aagatatgac ctaagagtct tgatcaatga gatatacctc tgaatcctga
      841 aaagaccact taatcgggtc ggatacgttt ttgcggctga tccgtagagg tggcagtgat
      901 atgtgccttt ttcttctacg gaggccggaa gggttgtctc cgtcgagctc ggacgcgtgt
      961 atgactcgag attcaagtaa atgagcaatg tatcctgtgc cttagtcgga agacggcaac
     1021 cttacatttt ccaaatggtc acgcactgcg tggtgaatta agctggacgc attatcacag
     1081 tttgatgggt gttgaacatg gaatgatcag catagcctga tacccaaatc cctgatcata
     1141 ccgaacaagt ctgttgtttt tacttggaat tgttgtgcaa tgttcggaat ctttgctttt
     1201 ccggaaggat tgttcgcatt gatatgtaca gctttttcaa aagtgacaat ggtaaatcca
     1261 tgcactacag cagcggctat caaccaagga tcagcggttg atccatctgt ccactgtcga
     1321 agtgcttttt ccgtataaca cgaatcttgt tggatgaact gaagaatttc tgcgtacttg
     1381 ttgacgatgt cttgtttgcc atgattgata tatcttttga tagtgaggct ttccatccag
     1441 ttggccaagt catccttctt tgcgggtttc aggatttctt tcttcacaag atccaagatt
     1501 gcgatggaac cttcttcgat tttctcagcc attgacttcc agaattctgg cgcaatatcg
     1561 aacggatagt atgaattctt tggtgttatc aaagaattcg tgtctatcaa gtaaatggtt
     1621 tcccgcgaca tcagaactcc atcctttcga caagttttgg aaaggtatgt ctgttggtat
     1681 cggtaagctt gaaggcttcg gagtagaggg ttttgcctcg agaaacgcta tccactaaga
     1741 aagccagaaa ccttttgtca atccggcttt cttttgttcg gtaaaaatcc ccacctgttt
     1801 tcttggtttg tttttgttta tggaaatccg ctcttgcaat ccggacgatt tcatcatatg
     1861 cgggctgctc aattctcccg gtgtccaacg cccttcgagc aatgacgatg cggccacatg
     1921 taaaataacg agcgacgttc tcgacgatct catctatgga tccggattgg tcgctatgag
     1981 cgtcccatat gctgccgaat agactcgctg gtacaaggat ttcagccgct accgcattgc
     2041 agagcgtctc ctgttttttg accccgttaa aggtgctgta cctattgtcg ttgaacaggt
     2101 tgtccacccc aaggcagata tgaacgaatt catggaggag agaaaacaac ctaccgctgg
     2161 cagaatccgc tgcattgatg aaaatcaatg gggcgaactc atctatgaga gtaaaagctc
     2221 ggaattcgtt gatatcgagt ggtcgatgtg tgttgttgcg gacgacaccg ttcatcatga
     2281 caatgacacc ggcttcactg attgtatctc tgagataatt gaaggagttc gctgtcgtct
     2341 taaaccagtc tatggataac ccaagaactt cccggatata ctcggccatg accatgacat
     2401 tggtttcatg tttgagcttg ccgacgatcg gattgatctc agaaccttcg gcaatcagat
     2461 aatctcgtgt ccaatcgaca atttcttcca tatcggcgat ggtatctatc agatccctgc
     2521 ttggattatc aaattcttcg ctcgctatgg ttctgtattc taaaagcttg atttcttctt
     2581 ttggtggcgt tttgaggaaa aaatatccca agggaatgcg ggtctttttg cttatatctt
     2641 ggatctcatt gaatgtaggg attttctccc ctgttttcca ttgattcaga gatttgagag
     2701 gttctgtatg ttctcccact tggctgataa cccattcaac cacttcaggg gaaactgcat
     2761 atgttgcttt catcgtttga ttaaacctct tggacgctgc ccttcaatct ttttctcgac
     2821 aatcattctc tatggacagg ataccggtgg agtaggagtt tttcaatgct tatcgatatt
     2881 tgtcgtcatg ggtgcttatc gatatttgtc gtcatgggtg ataccgattc gtctacaacc
     2941 tgtttgacag cgtcggatta tctttttcca tctagcaacg ggttaaaagt ttccattttt
     3001 caaaaatctt tacttttttc tatttcgtta tataataagt aaagatttag gggatgtttc
     3061 catgtatatc tacgatggcc taaacaaaaa gctgaaagag agaaatctaa aaaaatccga
     3121 tttgacctcg gtgctgagca tttcttcacg tacccttgcc aaaatctcaa agggagaaaa
     3181 actggcagac agcgtgatgt caaagctttg tacctatttt tcatgcagca aagatgatct
     3241 gtgccgcatc gtatcagaca atcccatcct tcaaacactg cgagaggaaa aggagcacaa
     3301 gattccgggc ggtctctacc acgagcttca gatccgtatg acctacaact ccaatcacat
     3361 cgaaggcggt acgctgagtg aagagcagac acggcgtatt tttgaaacaa atacgcttga
     3421 tgctgaagat ggtcttcctg ttgatgacat catcgaaaca gtcaatcact tccgtgccat
     3481 tgactactgc attgacatgg cggaagaacc gcttaatgaa gagatcatca agcatctgca
     3541 ctatctcctt aaaagcagga caaagaacga aactcttttc tggtttagag tcggtgatta
     3601 caaattgcgg cccaacgttg tcggcggaat tgaaacgacc gccccaaaag atgtagcgac
     3661 cgatatccaa gcactcctgt ccgcttatct gaaaaaacaa acggtgactc tcaaagacat
     3721 catcgacttt cattaccggt tcgaacgaat ccatcctttt caggatggga acggtcgtgt
     3781 cggcaggctt attgcattta aagagtgctt gaaaaacaat ttgatgcctt tcatcatcga
     3841 agaccggaaa aaacagtatt attaccgcgg tcttgcagaa tacaccaatg agccgggttt
     3901 cctggttgat acctgctacg atggtcagga cacgttccgt gccctggttc agcgtttcga
     3961 tattgatgtt tccgctttgg atgaaaaatg atcgtgccag tgaacagaac cttcaatggc
     4021 aggataccgg tggaggagga gttttcaata cttctcaaag gttgttccac cgttgtaaga
     4081 ccgggaacca tcgagctggc tggcatcaat ggtctgaaaa aaccgggttt tgacagggtg
     4141 ggggaaatga aagggccacc ctttcggatg gctccctcag gtgccaatcg gattcgaacc
     4201 gatgcataaa ggttttgcag acctctccct taccacttgg gtatggcacc gaacacgagt
     4261 tgaagataac aggttttagt accttgcgca aggggagtct tggatggcat ggtctgatgc
     4321 tccgtacata ctctcatcca tctgtgtttc tccaattcac cgaattcgct tttgaagatc
     4381 ttcaatcgaa ggcaactgtt cttttaattc ctctggcaaa tggcttgtga tccgatactc
     4441 acttactccc atcggcttcg agatatcttt cagtgcgtat tctgcaacca agttattctt
     4501 acttttacat agcagaagtc caattgatgg attatctccg tcctttttag agttccatca
     4561 acagcagata gatagaaatt gagctgaccg gcatattccg gcttgaattc acctgttttc
     4621 agttcaatta ccacatagct tcgtagattc agattgtaga agagcaaatc gatataaaaa
     4681 tcatcaccgc ccacattcag ctggtattga tgtccaagga aggcaaagcc cgttcctaat
     4741 tccaaaagca gttttgtgat atctttgacc agagcctctt caatagctcg ctccaccatt
     4801 tgctccttaa aaggaataaa gtcaaatatg tacggatctt tcatcgtctg aacggctaat
     4861 tcgctgtgtg gtgccatacg tcgagcttca aaattactga ctttttccgc taacgcttga
     4921 cgctcgtaca atttactttc gatttggtga acgagaactg cgtgagacca accattttcg
     4981 actgttttct tgatatacca aatccgttcg tctagatcat ttactttatt caaaatagcg
     5041 atgttgtgtg accatgggat ttgtgcagat accgtctgca caaattgttc atcaggatat
     5101 tcctgagcaa atttagccat atatttcagg tttcgcacag aataaccctt ggctctcgga
     5161 aaggagatgc gaatatcttt cgccaaatta tcaatgaact tattacccca ctctttatgc
     5221 tcattaatat gctggccaat gcggtaatac atgagtacga gcccctgatt caggctcaag
     5281 ctcgccttat gctgagccgt caagatttcc tgtttcactt gctcgacaag ctcaaaatat
     5341 tcattgttgt taaacaacat ctttctcctc cagcataagt acacttatag tcattgtgtt
     5401 atgatcctga tgtaaaatgc gatgattttt atgacacatg ttgttctgat agtgattatt
     5461 aaaccacgca ggttatttaa taaaaagttc agtcaccgaa cagtgaatga aaatcttgac
     5521 gaacaataat aaaattatta aaattaataa aattattaaa acatggtaaa gggggatacc
     5581 ctatgcaacc tgtagaaaaa acaagaactg taaaagtgcc tataagtgtt agttttgatt
     5641 caactcttgt tgaacaaatt gattctattt gcaaggaacg tggttgtact cgaagttggt
     5701 tcgtaaataa ggcggttgaa gaatatatgc ttgaatacgt ggaagacaga gacgactatg
     5761 aaactgctgt cgctgctttg gaagaatttg agaaaggtga cagaaaaacc tatacgcttg
     5821 aagaagtcta tgcagaggct gatctcctgt gaacattact ttttctgaca aggcgaaaaa
     5881 acaatttgta aaacttgata agcctgttca aaatcaaatg cggaaatttc ttgatagtct
     5941 taaaaattta caaaatcctc gaactcgtgg aaaagaactt gtcgggaacc ggagaggaat
     6001 ttggcgttac cgtgttggtg actacagaat tttatgtcaa ataaaagata aggaactttg
     6061 tatttttgtt gttgaagtcg gtcatagaaa agaggtttat gacagatgaa aaatgctaaa
     6121 aagttcggtc accgaacttt taagatatat aaaaggtgag ttctttattt gtgagcttca
     6181 caccattaca gaaatcctac cttccttgcc gtaccaagtt gattcccata agctctcctc
     6241 tacttcacct tatttattat gctgtcaagt agagtctgat cttcgatttt cgtgattcca
     6301 aagctctttt tgccggcatc tttgattgaa gcccctatat gataaacttc cgtgtcatct
     6361 ataatcaaaa agcggtcatg aaaatcattt ctcgttttta ctagtaaacc ctgatactga
     6421 gcgttgaatt tggagatatc cttacttgtt aaacttccct ttcctgagcc tgcaataagg
     6481 acggaaactc cattattttt cttacataac aggtttaggg tatttgtatc cacataatta
     6541 tctatcaata cgagcttgtt ctgggctttt ttgattagat ccacaataaa gctgaaggca
     6601 tcgtagatct gtccatcaaa gaaaatatgc tgctttacct ctgtcctggt tgcaatataa
     6661 ttaaaaactt gctccagctt tttatctgtt tcgatttgtt tcagctccac cctgtctaac
     6721 ctagagaaaa gctcttgatt tgaaacgaga aacctccgca tttcgacaaa gctatccata
     6781 attctaatac ttacttcgac agctacatta cttctaagaa cagatgaaag cattgcaatc
     6841 ccttgttccg taaagacata tggcatgtat cttcgtccac catgtttatc accatcattt
     6901 gaggtcgcaa tttgcgacct caagtcccta tattcatctt cagtcaactg aaacataaac
     6961 tgtggaggaa atctattctt atttctattg acttgttcat tcaatctctt ggttgtcacc
     7021 tgatacaaat cggcaaggtt gctgtcaatc atgacctgcc tacccctaat ggtatatatc
     7081 atgtcctgaa tttctctctt ttctactaac acaggtactt tgtcgtcagc catcgttacc
     7141 tccggattct ctataaccgg ccgcaatgtg cgaccacttc catatggcat catgcactgt
     7201 ttccgtctat acggaagcaa aaatcatctt acctgattat gtggtggcaa tccctcgttt
     7261 gcttgaagtt taccgatgtt cggatttcgt gctttgtgga catcatcaag tagggacgca
     7321 aacacatcgt cagaaggcga cagatgcatg gtgtattgac atgtgaatgt ttttgaatgt
     7381 attgtataaa taagttcaaa aacaatcagg aagtattcat ggcaaacatt ccggcaagtc
     7441 gccaacagta cattctgtcc cggattcagg acgaggggac ggtgaatgtc aatcagttga
     7501 gcgatgcctt ggacgtcagc gagctgacga tccgccggga tctggaccag cttgccaagc
     7561 ggggcctcgt cgaacggacc cacggcgggg cgagcctcag gcggaatctg cccgtcgaac
     7621 ctgattacct tcaaaaagca tcggaatttc cccttgagaa aagcatgatc gggcaaggcg
     7681 tccttgacct ggtcgaggat ggcgatacct tatatatcaa cagcggaacc accaccttgg
     7741 aagcgatccg ggcgctggtc gcttccggaa aacggctgac gattgtgacg aacaacatcg
     7801 atgcggcgta tgtgtgcagg gaaagccagg cgatccgtct ggtgctggcc ggcggagtgt
     7861 accggacgcg gagccattcc gtctccggct ccctctcttc cctagtcgtc gagcagatct
     7921 gggcgaacaa ggcgatcatc ggggtggacg gattctccgt tcaggcgggg ttgaccacgc
     7981 cggtcctgga ggaagcggaa accacccggg cgatgatcaa gcacaccgcc ggaaaagtca
     8041 tcgtggtcgc cgcggcgaac aagatcgggg tggtatccaa ctataagacc gtctcgcttg
     8101 aggatgtgga cattctggtg accgatgaaa agggcgccga actggtaagt caaatggaga
     8161 tcagtgacga tctttcaata gtagtagcac cgacgaggag gagcgaatga agtacttgga
     8221 tgaatatgca aagcagtatg aggactgtgc atggctgccg tttgaagagc tggaagcctt
     8281 catgaaagag gcgttgatcc acgcgggggt tcccagggaa gacgccgaga tcatcagcga
     8341 tgttctgatc gaaagcgaca agcggggaat cgacagccac ggaatcggcc gcctgaagcc
     8401 gatctacatc gaccggatcg acgacgggat catgctgccc accaccaaga tcgaagtgct
     8461 cagggacaag gccgccgccg ccgtcctcga cggacacaac ggcatgggcc acgtggtcag
     8521 ccacaaagcg atgagcatgg cgatcgaaaa agcccggaca tacggcatcg gcatggtaac
     8581 ggtccgcaac tccaaccact acggcatcag cggctactat gcgacgatgg cgaccaggaa
     8641 gggattgctc ggaatcaccg gcacgaacgc ccggccctcg atcgccccga ccggcggcgt
     8701 cgagaacatg ctcgggacca acccgctggt cttcggaatg cccaccgatg aagagttccc
     8761 gttcgttctg gactgcgcca ccagcgtctc ccagcgcggc aagatcgaag tgtacggccg
     8821 cgccggaaaa gagctgcccg aaggatgggt catcgacaac gaaggcagaa gccgcaccga
     8881 cacccttcag gtgctcaagg acctgaccac cggcaaggcg gccctgaccc cgctcggggg
     8941 aatcggcgaa gtgaccgccg gatacaaagg ctacgggtat gcgacggtcg tcgagatcct
     9001 ttccgccgca ctgcaggcgg gttcattcct gaaagctttg aacggcttcg acgagaacgg
     9061 caaccagatt ccttatccgc tcgggcactt cttcatcgcg atcgatcccg agttcttcat
     9121 gggccttgaa accttcaaga agatcaccgg tcagattctc cgcgatctta gggccagcaa
     9181 aaaagctccc ggcgaagact atatcttcac cgcgggagag aaagaatatc ttgcgtacca
     9241 gtaccgcaaa gaacatggat gtccggttcc tccgtccctg cagaaggtga tgatcaccct
     9301 gcgtgaccgc tggaatatgg acatgagttg ggattttgag aaaaagtgag gagaagaata
     9361 agatgaatca agatctgctg aaagaaaagg cgttggccta tcacatccag gacggagttc
     9421 ccggaaaggt ttcggtcgtt ccgagcaagc cctgccagac cgccgaggat ctgagccttg
     9481 cctacacccc cggggtggca aagccggttc tggagatcgc cgcgaatccc gaggatgcct
     9541 acaagtacac gtcaaaggga aatctggtgg ccgtcatctc caacggaacg gcgatcctcg
     9601 gtctggggga ccggggtgca ttggccagca agcccgtcat ggaagggaag ggagttctct
     9661 tcaaacgctt cgccgacatc gacgtgttcg acatcgaaat cgacgaaacc gatccgaaga
     9721 agctgatcga gatcatcaag aagatcagcg tgacgttcgg tggcatcaac cttgaggata
     9781 tcaagagtcc ggaatgcttc gagatcgaac agactctcat cgaagagtgt gacattcccg
     9841 tcttccacga cgaccagcat ggaacggcga tcatcgccac agcgggtctg atgaacgcct
     9901 gcgaggtggt ggacaagaag atcgaggaag tcaaggtcgt catcaacggc gcgggagccg
     9961 ccggcatcag ctgcgcgaag atgttcctga acgcgggagt gaagcgacag aatctgattc
    10021 tctgcgaccg ccacggcgtt cttcgccggg accgcttgga aggcctttcg ccggagaagc
    10081 aggacttcgc aatcgatacc gagctggaaa ccctcgccga cgcccttaag ggtgcggatg
    10141 tcctcatcgg tctttccgtc gccgacgtgg tgacccccga aatgctgctt tcgatggacc
    10201 gcgatcccat cgtcttcgcc atggcgaacc ccaatccgga gatcgcctac gagcttgcga
    10261 tggcgactcg aagcgacctc gtcatggcca cggggcgcag cgactacccg aaccagatca
    10321 acaacgtcct gggcttcccc ttcatcttcc gcggcgcgat ggacgtccgg tcccggatca
    10381 ttagcgaagg gatgaagatg gccgccgcca aagcgctcgc cgacttgacg aaggagccgg
    10441 ttcccgattc ggttgcaaaa gcgtacggcg ggaagaagtt caccttcggc cgggagtata
    10501 tcgtgcccaa gccgttcgac ccccgggtca tcgaatggga ggcggtcgcc gtggcgaaag
    10561 ccgcctgcga ggaaggcttg gccgcagctc cgatcaccga ttgggatgca taccgcgagt
    10621 cgctgaaaca gcgcatggct cactggtggg cgtaagcgca atcaggaagg gtgatacgat
    10681 acaggctgcc tccgggcagc ctgatccgtt ctattgtcct tcgatgccca ggatctcctt
    10741 catccgctcc ttcgcgttgt ccagaacgtt ggtgtattgt tcttcaaggg ttttgagctg
    10801 tttgtccaga accttcatga actcggggtc ctgctccggg cgaaggacga agttcgggcc
    10861 gtactgggcc ctgagctgcg cttccttctg ctggaggtgg ggagcgaact gctccttcag
    10921 ccgctcgatg agatcctcct gggtttcgac gtattgggtg aagaacccct gcatttgagc
    10981 gaggagttct acaagctcct catcgtcgtc gctgatcatc gccccgaggg cgatcagctt
    11041 ggggatggtc tcggcgtagt tgggggtttt cggcagggtc aggttggaaa gaagggtgag
    11101 cgccattccc tctttcacat gggccttggc ctcattggag taggaatcgt acttcttctc
    11161 tgcctcttcc ttcgttccgt cgatatcgag caggaaggcc gcggcgatct gccgcccctc
    11221 cttgatggtg aggtctttct gtatcttttt ggggtccgcc tgaatcgcgt tcgcccgctc
    11281 catcgcgatc tcccaagcgg atttgatgat tgccatagta ctctcctgcg tgtaatcgta
    11341 tgtattgcaa agatagtaca gttgccgctc ttggacaagt aaacccttta atgatacgct
    11401 caccacagcg attccgatga tgacaatgaa taaccacatg aagaaacact cgtatccgcg
    11461 ctactatgac gaaccctacc gaaccaccct ggaaacgagg gtcgtgtcgg tcgaaggatc
    11521 gaaggtggtc ctggaagaga cgatctttta tcccgaaggc ggaggtcagg gcggggattg
    11581 gggaacggtg aatgactgcc ccgtccttga taccgtatca ggcgatgatg aagagatcat
    11641 ccacctggtc cgggatcccg ccttcaaagc gggtgatcgg gtcgttctca ccctcgattg
    11701 gaatcggcgc ttccatttca tgaagaacca caccgctcag cacgccgcaa gcggcattct
    11761 cttcaagcac ttcgggatcg gaacggtcag cgtccatctg ggaaccgaag tgatgatgat
    11821 cgaagtcgac cgacaggagg ttcccgattc cgtctgtctt gaacttcaat ccctggtgaa
    11881 ccgggcgatc gtcgaagggc atcccgtcac ttcggaaacg aaaagccatg aggatgccga
    11941 agcattgggc ctgaggcgcc cgatcaaggt ggagggggat gtccgcctcg tccacgtcga
    12001 cggggtcgat acgatcgcct gcggcggctt gcacgtttca aacacccggg agatcgggct
    12061 ctttctctac gagggtcagg aaaagatccg cagccacgcc cgcctgatct tcaccgtcgg
    12121 agaggttgcc tggaaccggg ttcttctcaa ccggacgatc gtcgatcaat tggggaccct
    12181 ccattcatcg cctccggagg cgctggtgga aacggctgcc aaagcctgcg aggccgccgc
    12241 atcggagaaa gcctttctgg gccgcctgcg gaatcggctc gcttcggtgt tgctggattt
    12301 gaaacttgcc ggggaaaccg acccgcttcc cgtcatcacc tgggaggtgg aggatgagat
    12361 caccctgaag gacctcgctc aggcggcaag ccgggtcgcg aatctcgccc tctgcgccac
    12421 cttgcgtctg gaggacggaa agctcgcctg gctcatcgtc ctcaccggcg agggagcggc
    12481 caaattggat atcgcaaagc atcgaagtga ccttctttct tcaatcaacg ggaagggcgg
    12541 ggggaaagcc ccgatctttc agggcgttgg tgaaggcgat cccgccgagc tgttcgctcg
    12601 gtttagagac ctgttgagat gagtgaaaaa caacaaacgg aaccaatcga gatcgacgaa
    12661 ctgcaccgga tagatcccaa ggatggcgag ctttcgtttc gcgagctctt caaaaagtcc
    12721 atctgggcga tcgtggtgct gatggcggtc accttcactt cgctcttcct cttcaagaag
    12781 ctggggtggg cggacgccga ggttctgcac gacttcatcg acgccttcgg ggcgtgggcg
    12841 gtcgccggct acatcttcgt catggagttg acgatgttcc cggtcagcgt ggatatggtc
    12901 tggcccttcg ttctcacctg gtcggtgccc aaggcggtca tcgtcatggg aaccgccagc
    12961 gtggtcggat ccttttgcgc ctacctcgtc gggcggcttc tggggcatat ccccatcatc
    13021 aacaactatg tgcggcggaa cacgaaaccg gagaccgctg cgatgatgaa gcgctacggg
    13081 gcatggggcg tggcgatcgg ggggatcgcg ccgctgccgt tctccacgat ctgcaccctt
    13141 gccgggatgg cgaagctgcc ggccctcagg gtcctgctcg ccctgcagat ccgctatgtc
    13201 cggatggggc tgtacttcct gatcttcagc ggattcctca gtctcggttg aggaacgcat
    13261 cgatctcttt cgcgaccatc gtcgtcctca ggaccgcatc gccgatatac gcttcgacct
    13321 tcatgaattc aagaacttct gcctcggtga cgaaggccag aaccttcttg tccttgctca
    13381 gcgacttggc aaggggattt tccttgccgc ttcggaccgc ctcccacgct ttcaggctgt
    13441 ggttgcggat caattcgtgc atcgcctgac ggtccgctcc gcggcggccg agctccatga
    13501 ggaggcgctc gcttgcggca aagaggccgt acgcgttgag gttccttccg accgcttcct
    13561 catgaaccgt catccggccc accgtcctga ttgcggtgac gaggatctcg tcgacggcca
    13621 ggaagatctc ggggatcgcg atccggcggt tcgccgaatc atccaggctc cgctccagga
    13681 cggtggtcgc ggcgttctgc cagaaaaggt cgacatgggt cgagacgagg cggcagaggc
    13741 tgttgatctt ttcgctgttg atcgggttgc gcttgaaggg catcgccgag gagccgacct
    13801 gtttggaggc gaagtattcg ctccactcgc cgatcggggg gctcatgaga atccggaaat
    13861 cgaagaagaa ctttgaaagg gtggcggcaa gacccgagag agcctgggcg acgcgaaggt
    13921 cctgcttgcg ggtgtagatc tgcgtcgaag cggtgtacgc cgcaagtccg atctcttcca
    13981 tgaccgcctc ttcaagctcg atggcggtga ggccggttcc ctccagaagc tgggcatagc
    14041 tggcggcggt tcccaccgcc cccttcatcc ctttgccccg gatcgaagag cggacccgga
    14101 tcagctcctc cagatcctcc ttcaggtctt gggcgctttg ggcgaagcgg tagccgacgg
    14161 tggagggctc ggcgggctgg atgtgggtga acgccatcgt gggaagttcc cgatatgctt
    14221 ccatcttctc cttgaagagg atgagcagct cccgggtctt gctgatcgtc agatccagcg
    14281 cttctgtaag gcggagggca tccatgttgt ccaggacgtc catgctggtg gctccgaggt
    14341 ggatgatctt tcccccgttt gggcactgct ccgcccacgt gtggatttcc gccatcagat
    14401 cgtggtggat ctcgctttcg atctgtgtcg cccgggcgat gtcgatccga tccttcgtcg
    14461 cttccagttc ggcaagctgt tcttcggtga ccagaccgac ccgcatctgg gcccgggcaa
    14521 gggcgatcca gatcgtccga aggagcgtcc gcttgtggac ttcgctgaaa atccggcgca
    14581 tggcttcgga gccatagcgc caggtgaagg gagagatgaa tgtctgatgg ttgaactctt
    14641 gcatgggtaa actgtacaag agaggccttt cggggtcaat ctacgtagcg gatcgtctcc
    14701 caggctttgt tgtacatctc gaggttcgcc cccagatcct ccttcagctc gtagttcgcc
    14761 agctgttcga cggtgtagaa gggggtggtg gtccgatacc gatccgcttc gatgttgacc
    14821 gatgcgggga agtggaaccg atccagaagc tgggcgtagt tctccgggcg gtggatgaaa
    14881 tcgatgaagg cgagggcaag ctcgtagtgg ggagcccctt tggggatgca gagggaatcc
    14941 atgtacagcg gtccgccttc ctcgggaagg aagaagtcga cctcggccca cttttcctcg
    15001 ggaatctctt cgaagatcgc ttcggcgtag ccgtggctga catagtattc gccgctggcg
    15061 aatcctttgg cgaacgattc ggcgtcgaac ttcaccaggt tcggcttcca ctgatcgttg
    15121 accagcttcc gggcgcgctc aagctcggcg gggttggtcg tgttgacgga gtatccgaga
    15181 tggacgagcg catccccgag cacttcgcgc atgtcatcca tcatgaccat ccgatccctg
    15241 aagcgcttgt caccgaagat gttccacgat ttctcatagt tggaggcgta tttcgtgtgg
    15301 acggcgatgc cgcttgctcc gaggtaatag ggaaccgagt attccatctt cgggtcgtac
    15361 gtcgccttgg agagggcgaa ctcggtgatg tacctgctgc tcgggatctt ggaaaggtcg
    15421 agcttttcga gcatccccag cttcatcatg atggaaacgt agtcggcgga ggggaagatg
    15481 acgtcgtacc cgctttcctg gctggaggcg cggagcttgg cgaacatctc ttcattcgaa
    15541 gcgaaatagt cgaggacgac atcgactccg tactcctttt cgaacgcttc gatgacggag
    15601 tcgggggtgt aataggacca gttgtacaca tagagcttct gatttccgtt ctcgttggcg
    15661 ctttccttgg cgcatccggt aagcagaaag acggtgaccg caagtgcgat catgagtttg
    15721 gtggcaggtt tgaacatggc aaacctcctg tctggattat gctgcccgct gaggagaaca
    15781 tacgccatac cctcgggcgg tggtgggttt tcagattagt ggacgaagca atatctcttc
    15841 atattcttgg cgttttttta atctgaccta tcattatgct ttgaagggtt tgagtcaatt
    15901 gttttttcag gtcaatcgga aaaagttttt acgattgacc cttgtgttca ccgaggggcc
    15961 tgcccggaat caggcacccc cttttcaaca ggaacgtgtt gagatgaacg tatttccgtg
    16021 gtagaacgat agatgcaacc tgtccggttc atgagaatgg aaaatgagtg aattcaagaa
    16081 agatgaaaaa cgtgccgctc cttttttgga gatgaaaaag gtgagtaaaa tctatgctcc
    16141 ctctcgtcaa catcatatca aagcggtcaa tgatctctcg ttcaccatcg acaagggtga
    16201 gttcgtaacg cttgtcggcc cgtccggctg cggaaagtcg accacgcttc ggatgatcgc
    16261 cggctttctc aggccgacga gcggcgacat cctcatcgaa ggcaaatcga tcacggcgtt
    16321 gggaccggag atgagaaaca ttcccatggt tttccagaac tacgccctct ttccccatat
    16381 gaccgtgttt gaaaacatcg cctatgggct ggaagcgaaa aacctgccgt tcgatgcggt
    16441 cgcgcatgac gtggcgatga tctgtcaaat gcttaatctg gtcgggaccg agacccgcca
    16501 tcccaatgag ctgtccgacg gacagcagca gcgggtcgct cttgcccggg cgctggtcct
    16561 taaacccaaa ataatgctgt tcgacgaacc gctttcaaac cttgatccgc gcctgcggat
    16621 tcagacccgc ttcgagatca agcggatgca gcagcttctg gggatcacgg ttctctatgt
    16681 cacgcacgac caggcggaag cgctgagcat tcccgatcgg atcctggtga tgaaccgggg
    16741 aacgatcgtg caggagggga agccccgagc cctgtacgat cgccccggca ccccgttcgt
    16801 ggcggacttc atcagcaatg cgaacttctt tgacggattt gttctttccg catccgcaga
    16861 gacgatccag ctgagcattc aagagatgga attcaccgta tcccgggacc gatgttccat
    16921 cttcgtcgag caagacgatt cccttctggt ggcgatcaac cccatttccg tcggcatgtc
    16981 accgtttgaa ggcgagtgcg ggttcaatca gtgcgtaggc atcgttcgct cttccctttt
    17041 cgtcggccag tcattcgaat atcgggtaag cttcggtgat gagacgatcc gggtgctgca
    17101 tccgaacctg ctcggaggag ttcgggagtt tgaagagggc acgccggtga ttctcacctt
    17161 ttaccccgag tccttcaatc tctatggccg cgaggtggat catcatgaac gctaggctcg
    17221 tcggcaggca gattcttgag cttgttctcg catccttggg gacctgtctt cttctctctg
    17281 tgagcaagac ccttgtcgtc gggtgcacgg ttctgtacct tgcccttcgt ccggcgaacc
    17341 ggtggcagat ccgcctcgtt cggacacttc tgttgtgggg ggtctatgcc ctcgtttcat
    17401 cctcccgggc tttggatgtg cttacggtat atgcaggtgc gtccatcgtc gcggcatgga
    17461 cggatcggcg catccgttgg aatcgcaagc accaaacgca gcccgggtat gagcagctcc
    17521 tttccacgct cgcccatgag atcaggactc cgcttacgat catgcagacg acgcaaagcg
    17581 ttctgatgga acagagcgcg ggaaagctca acgagcagca gcagaagttc atggaatcca
    17641 tctacatcaa cacgcagcgc ctgatcacct tcagcgagca gatgctgacg ctgatcaagc
    17701 ttggaaaaga ttgggagccc gacatgacga aatcggtcga tctcaagctc cttgtccggc
    17761 aagtcatcga aacgttgcag ccccagcttt cgatccgaaa acaggaggtc tcgctgcagt
    17821 ttcccgccct ggtgtcccac ccgagggcgg atgaggcatg gatccgccag gttctgatca
    17881 atctcatcca taacgcctca aaaaacacga aggagggagg actgatcatc gtctccgtca
    17941 cccaggatga ccggcagatc gttacgactg tcaccgacaa cgggcaggga atcgagctgg
    18001 cggggcgcga ggcgttgttt caagagtttt ttcaggaaag gaaggactcg cagcaggacg
    18061 gattcggtct tggactctca atcgtccgtt ccatcatcgc caagcatcag ggaaaggtgt
    18121 atatcacaag cacacccaat caggggacga tggtctcctt cagtctacgg gtaggggcgc
    18181 catggtgaaa aaccggatcc tcgtcgtgga tgacgagcag gcgatcgtgg agcttttgca
    18241 cttcattttg acgaacgcgg gatatgaagt cgcctctacc gggcgagggg atgaggtggc
    18301 gcccctggcc cgggagtttc atcccgatct ggtcatcttg gatatcggtc ttccgggctt
    18361 gagcggattg gaagtggttc cactgcttgt cgagctggat actccggttc tcgtgctcag
    18421 cagtcatgat cgggatgacc agatcatcga ggggttggag gtcggggcgg aggattatgt
    18481 ctcaaagccg ttcaaccaca aggagctgct gcttcgaatc gaaaagatca tccgtcggac
    18541 gaaggtcgag caaatcattc agatcggctc gatcatggtc gatatccgca gacgggtatt
    18601 ggagatcgac ggacaggaga tcaccctcac tcccaccgag ttcagaatca tcgaatatct
    18661 ggcgctccat gctcatgtcc ccgtatccgc cgaagagctt ctcaagcagg tatggaatgc
    18721 cgaggagtgg atccaggggc cggaaatggt gaaggtgaat atcagccgcc tccggaaaaa
    18781 aatcgagccg aacccctcga caccgcggtt tctggtgaac agatggggat tcggatacct
    18841 gcttaccgac actcaagagg tctcctagga gttttgttac aaagctgtaa cttgcatttc
    18901 caccctccgt ggcgaaggat gaaggtggag ccgaagtatt tcggccaact acctaaggag
    18961 gatttctatg aaaagcatga agaaagcctt gatcgtgtta agcatgattg ggttgcttct
    19021 ggccccggtg ttcgccgccg gtgtccaaga gaaagcaagt accacgctga acgtgtacac
    19081 gatcatgcct gaaaagtatg caaccacggt ttttgaggag ttcacgaagg acacggggat
    19141 ccaagtcaat ttcgttcgcc tttcatccgg tgaagccctt gcccgattga tcgccgagaa
    19201 ggcgaacccc caggtcgacg ctctctgggg cgggccggcc gatacctatg aggccggtgt
    19261 tctcgaaggg gtgttcgagg tgtatgtgcc ctccgaagcg tcggcgattc ctgcccagtt
    19321 caagtcttcc aaaggatact ggaccggtat cggcgtcatc ccgctgacct tcctgagcag
    19381 caattccttc ctcaaggaga aggggttgca gccgcctacc agctggaacg atcttcttga
    19441 tccccgctac tacaacgccc ttcagatggc ggacgtccga accagcggaa cggcgaccga
    19501 acgagtctat tcgttgattc aggtctgggg cgaggataag gcgtacgagt atcagaaaca
    19561 gctgcacaag aacatccagc tgtacaccaa gagcggagcc ggcggtgcga tgccgatcgc
    19621 aaccggtcaa gccggttccg gcatcttcta catcgttgac gctcttgata tccagcaaca
    19681 ggggtatgac ctggtgattt catatccggt cgaaggtgtc acctatggtg tcgaaggctc
    19741 cggcatcgtg aagggtgcaa agaacctaga tgcggcgaag aagctcatgg attgggcctc
    19801 ttccaagcgc ctcggacagg tcatggtcga caataagatc aactacattc cgacccggcc
    19861 cgatgttgtg gtgaccaatc cggctctcga catgtccaag gtgaagcttc ttgaaaccag
    19921 cgtcgcttgg aagggcgcga accgtgaacg tttggtccag cgctggattg atgaggtcat
    19981 ccaaggtcag tgacgccctg ctagagagag gaaggtaaga acgtggaagc acagatcccc
    20041 aaaagagttc gtagttcatt gatgaagcgc gatccggcgc tgttcatggc gctggtcacc
    20101 gtttggctct cctttgccgt ttttatcttc ctcccgtttc tccggttgct cggagccacc
    20161 ttcgtggttg acggaaagct gtcgctggcc aatgtcaaaa cggtgttatc caaggcttat
    20221 aatgtaaaag ccctctatgg gagcctggtt ctggctacgg cggtctccat cacggggacg
    20281 gtggtcgggt atctgttcgc ccttgccgta acccggactc ggatttcaaa ggttaccaaa
    20341 gggatcatcg gcgccgtagc gatgcttcct cttatttcac ctccctttac ctcaagcatt
    20401 tcattgaccc tcgcgttggg acccaacggg atgctgctga aggctctggg gatcggtgat
    20461 ttttccatct acgggtttct cggaacatgg atcagtgaga cgctcaccta tttcccggtg
    20521 gcgttcatgg tcatcacggc tgtgctcaac acgatgagcg ctacgctgga cgatgccgcc
    20581 ctctcgttgg gggcttcccg ctggaggatg ttccggacat tgacgatccc gctttcgctg
    20641 ccggcgatcg tcaactcgat tctgttgttg ttcggggcca gtctggcgga tttcgccacc
    20701 ccgctgatcc ttggcggaca tgcgttcccc gttttgccca ccgaagcgta tctcctcata
    20761 accggcatgt tcgacttgaa gggcggggcg acgatgtcgt tccttctgct ggttcccgcg
    20821 ctgctcgtct tctttcttca gcggatgatg ctcgggaaga aaagcttcgt atcggttacc
    20881 ggaaaaagcg gctcccgcac cgagttcgcc cggcttcccc agtctctgaa cgtggtcatc
    20941 gccgtggtgt gcctgatcgt ggtcgcgttc atcatctacc tctatgcgat catcctgtgg
    21001 ggctccttgg tgaaggtatg gagcgtaaac aacacgccga cccttgagca ctacagatat
    21061 gttttcaagc atggtcaaaa agcgataaag gacacggtcc tgatcgcgat catctccacc
    21121 gtcatcggct ccctgcttgc ggtggtcatc gggttcatga tccaacgcaa ggagttcgtt
    21181 ggtcgaaggt tgctggagtt cagcagcatg ctcaactacg ccctgcccgg aacggtcgtg
    21241 ggtatcgcct acatcatcgc gttcaacacg aaacctctgg ttctcaccgg aaccctttcg
    21301 attctcgtcg cagcctatgt cttcaggtat tatgcgaccg gcatccgttc cgtgatcgca
    21361 tccctggagc agatcgatcc cgccttggag gaggcatcgt tgagcctggg agccggaacg
    21421 gtaagaacct tcacccatat cacggttcct ttgatcatcc ctgcgctcat atccgggatg
    21481 cggtatctct tcatccactg catgaccgcg atcagcgcca ccattttcct ggtatcggtc
    21541 cgttggacct tgctcacgac gagaatcctt gaaagcatga ctgagctcca gttctcacag
    21601 gcatcggcgt tctcgatcgt cctgatcgcg atcgtgtttc tcgctgattt cattttgaag
    21661 catgtattga aattcacgac cgctcacatt actcggtctc aggagatttc ctatgaacaa
    21721 atcggcgctt gagttgattg gtgtttccaa gctcttccaa aaagatggcg aatccgtcct
    21781 tgcggtggat gctgtaggtc ttacggtcaa ggagggcgaa ctggtaacgt ttttgggccc
    21841 gtcggggtgc gggaagacca cgaccctccg gatggtcgcc ggattcgagc tgcccacaag
    21901 cggtcggatc accatcaaag gaaaggatat gaccaacgtc ccggtgaaca agcggggcat
    21961 cgggttcatg ttccagacct acgcgctgtt tccgcatatg agcgtgtata acaacgtcgc
    22021 atatgggttg aaagcgcgag gggagaagga tcgcgagata aaatccaaag tgcgggaagc
    22081 gctccgtctt gtcgggctgg agaagaccga gcatcgattc cccaatcaac tgagcggggg
    22141 agagcagcag cgcgtcgcgc tggctcgggt gatcgttatg gagccaagcc tcctgttgat
    22201 ggatgaaccc ctttccaacc ttgatgcgaa gctgagaatc cacatgcgga ccgagattcg
    22261 caagattcag caggatctcg gcatcacctg cctctatgtc acccacgatc agtcggaggc
    22321 tttgacgatg gcggatcgaa tcgtgatcat gaacaagggt aggatcgaac agatcgggac
    22381 gccgttcgag atctacagca atccgcaatc ggtattcgtg gcggatttca tcggacaggc
    22441 caacatcatc cgcttgaaga tcacggccgt cggctcggat acgatttccg tgcagattcc
    22501 ccggggaggg gtgttcaccg cccgggcatt gcgacccgag cggttgaaga aacaggaggc
    22561 gatctacaag gttggtgccg aggcatcctt gatcattcgt ccggagcaga tccaattggg
    22621 acccgctccg gcgaaggaag atcagtttcc ggccatcgtc gaatcggcga tgttcgttgg
    22681 aagccatatc gaatacttcg tgaagctgcc caacgattcg gtgataaagg cgttggttcc
    22741 ctaccagacc ggcatgcaca tcttctcggc gggggatgac gtggaactga gtttcgatgt
    22801 ggccagcgcc gtcttccttc ccaattagac tgtaacgttc agttttaggg ctcccgaaag
    22861 ggagcctgaa taatcttgca gaatacgggc aatggttgat tataaaatca tcgaagcaaa
    22921 tgaccaagga cttcgataag ggacctatag tttaattggt atagtaagat tgtatatgta
    22981 acttgaaatc acaaatcgtg atctcaacgt gccggtgggc tattttgaaa ttgcctcttt
    23041 aattgctgca ttaatctttt aagcagccaa taggaat
//
LOCUS       DAOSLI010000029        23068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2461_length_23068_cov_28.088037, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000029 DAOSLI010000000
VERSION     DAOSLI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 23068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23068
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2461_length_23068_cov_28.088037"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLF67_05615"
     CDS             <1..648
                     /locus_tag="PLF67_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HOE89525.1"
                     /translation="YTVDGGRRFEIQSECFNAVTVKVGFEGVSYHLGAARGRMVNALT
                     MASAFIGALPQAESPEATDGRYGYYCAHTIEGSIRHVDLTVYLRDFDLENLDRRIEVL
                     KGLARTIEGLYPGGKVDLQAKHIYYNMAQIAKDKPIALDTLFAAGKALGFELKNEVIR
                     GGTDGSRMANEAGIPCPNIFTGGYNVHSRYEWAALPAMVDATRLILKIIELGAAL"
     gene            645..1592
                     /locus_tag="PLF67_05620"
     CDS             645..1592
                     /locus_tag="PLF67_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89526.1"
                     /translation="MKRIGTLVLLLLVNSLIFSAIHVQRGPTIPGALIDHTEEALNTV
                     MGPRLKENQELRGFLTEEADRYLLTVNLDGRELMIPIPLSWEAEPSYISDFLVYDGLA
                     LLDDLPPLMIDVISKTSVLVENPDPSVRVGDRFWAIDARGNRIGLLGVRRVDESRLLL
                     QQEGGKTLQVGMGLVQGPEIPVYLSMSIDAQRVFGFHAGACIPLRLYPFSLAVEGGTL
                     GGREFSLGAGVKARVSFSQLFGTGWTPFRALSFSAAVSGGGAYSLGRGWGGYVQGTFL
                     LEYALGRWIIFAGGGERMRAFGSSVHDGLFFVAGTAYTF"
     gene            1596..2933
                     /locus_tag="PLF67_05625"
     CDS             1596..2933
                     /locus_tag="PLF67_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89527.1"
                     /translation="MKKLLPLMIVIPLLLASCRLAKDLPGSGTSSRASPLEGRASQVF
                     FHGEGRGLEPYQTLFARIEASIGFDLDQRYLDELIDTGGIADLGLVITNQYRKGDEIF
                     LRAQANEEVIERWRTELAQALQDRIDRIEELLREANLAYKANDDTITITRYLEAALVA
                     SGGPVGEKRYEPEALVTEAIRYIEPLQFVLTRSESASARTTVTLRRRTRLLAPRVMHA
                     PISVTYEAYNALGALYEDTLYFNSHDSGQLLFNPLNAAMKSEGVITFALNLDIPPLPR
                     DLALRVEEALKKVSIEFPYSLSHPFGPESIGLSLEEYSISGNLLPTTHAKTAFSEGSK
                     RDGIPILDVALERAAENELEDLVRQIPKNVRYTIIGSVGVLSFDKALDDVIAVVSGSF
                     ILYDSVRSAILYHSQDIEAVGWGATREEATARGFERFGSIAEYLLRTVLMDVR"
     gene            2967..3311
                     /locus_tag="PLF67_05630"
     CDS             2967..3311
                     /locus_tag="PLF67_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOE89528.1"
                     /translation="MTLREILELTEATLVCGSHHADDTITYGFASDLMSDVLTILKDD
                     IIMITGLSNMQAVRTAEMSDIRNILLVRNKQPSQAMIEIAEELGISISYTPYSLFRVS
                     GILYGAGLKPVY"
     gene            complement(3308..6256)
                     /locus_tag="PLF67_05635"
     CDS             complement(3308..6256)
                     /locus_tag="PLF67_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOE89529.1"
                     /translation="MAERQLAAGDELYGFILEEITPLAEYQGTGYLFRHIETKMEVYQ
                     VVNEDPECFFGYLFRTLPCNDTGVAHIIEHCLLAGSERYPVHDPFMTLLKGSANTFMN
                     ALTYPDRTVYPAASPLKKDFDNLFAVYTDAVFAPLLREETFWREGVRLVCNEEGCHYE
                     GVVFNEMLGVSADHDAIVSRSAVQALFPDNPYSFESGGDPEAIINLSYQEFRSFYDQY
                     YHPSNAKLFLFGRMEVGEKLAYLDEHYLRTRGMLQIDSIVPLARRWDRERAVTFTSPM
                     EEGGEEVKNASIILSWATTENDDSLSVMTLSTLVDILLGNPAAPLYKAIIDSDLASDI
                     SPESGMSAEFRQMPFIAGFKGIDPEDGEKAQNVILSTLKKIVRDGLDPELIETSLKRT
                     RFSQLEIPGGIPTGLRALTRATRAWMVDRSPSETIELVPTLNELEKRIKRNSRYFEDW
                     IKDHLLDNPHRILVTVKPDEEHQRRQNEAIAKRALHAAKQPGRNGMKEIIEQNRRFAA
                     FEEERDSTENLEKIPFLRLEDLPKEIRPNDHALKIIAGRELWIRTIPTNKIVYADIAF
                     TIDDLTEEEMLLLNLYTRLVQTTGLGELPYETVAQRIKRLTGGFSFFVEVGTSLQGEA
                     VHLLIVRIKTLAEDFDEALGFIQELLVGANVDDRKQLRFVLNNYKSEFSEVVTYGATN
                     FASSYATRTFSEAAWDAELIGGLSQWFHLTGLEADSLGGRLKALQTKLANRRRLVAHL
                     SCEEPLLEDSVKSFEMFITRFEDRGPLDKEPRSYEKVGPTSNPVVDLFKLPSTVSYSA
                     WALRSAKRGSVLQSAQVLLSQILTTGPLWETIRGQGGAYGVSASADVAEETFILSTYR
                     DPRIAGTFTDLKRVLEEAATRVFSEKEIEDSTIFIVGRELRPLSPGQDALLGFRRILY
                     SITDEFRLMRRTQLLELSNEDLNEGAKALLEQAERDGSNLVVLASGELLGKEAKKLEI
                     LNVESRRLPL"
     gene            complement(6353..6979)
                     /locus_tag="PLF67_05640"
     CDS             complement(6353..6979)
                     /locus_tag="PLF67_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE89530.1"
                     /translation="MRSSAAPPRGRFPVRRRSRCGLRYSTWFHGRTQPTSCQSICTGR
                     MGFVFQQMNVPANLNMIDNVVLPALHAKKSRKRELYRRARALMDDFHIGDLAERRVNE
                     VSGGQLQRACICRSLMMEPEIIFADEPTGALNQAAADVIEAFLQINLKGTSIMMVTHD
                     SRNAAVCDRILYMLDGEIKGELRLGKYVHDEAKEREKRTVRWLEEMGW"
     gene            6901..7788
                     /gene="nfo"
                     /locus_tag="PLF67_05645"
     CDS             6901..7788
                     /gene="nfo"
                     /locus_tag="PLF67_05645"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOE89531.1"
                     /translation="MYYIGAHTSIAGGLENALLEAQRLNATALGMFTKNQRRWAAKPI
                     EAEEADRFTEVLQETGFGSDRILIHGSYLINLGNPDREKQRRSIRAMADELGRAERLG
                     LKLVNTHPGSTLGLVSKEEGMKIVARGIDEALDESSKAILVIENTAGSGSNLGSTIEE
                     LAGILEATRNHARLGFCLDTCHAHVAGYDLSTVEGFENLIDRFDRLIGLEFLRGLHLN
                     DALSESGSKRDRHASLGEGTIGWPTFEHIVRDPRFRGIPLVLETPDPSIWGDEIAHLR
                     ALTFKNRVCEVYWPKRRNG"
     gene            7785..9278
                     /gene="glpK"
                     /locus_tag="PLF67_05650"
     CDS             7785..9278
                     /gene="glpK"
                     /locus_tag="PLF67_05650"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOE89532.1"
                     /translation="MKYLGAVDQGTTSTRFIIFDQGGAIVASHQLEHTQIFPQPGWVE
                     HDPMEIWANTQKCITEALKKARLRGRDLSGIGITNQRETIVAWEKESGRVYHNAIVWQ
                     DLRTSDHIEALKDSPNGPVIEQKSGLVLSPYFGGSKIAWLLEHVEGLRSDAESGKAVF
                     GTIDSWLAYQLSGKRIHVTDASNASRYLLMNIETLTWDDELLEIFSVPKCSLPTIVPS
                     SGMVYGHTVKEGPLGAEVPIAGMLGDQQAALFGQACFKPGMGKSTYGTGCFLLVNTGT
                     GLARSQNGLLTTVAYKIGDEKPVYALEGSVAVAGSLVQWARDNLKLVESAQQLDREAL
                     TVTDSGGVYVVPAFSGLFAPYWRSDARGVIAGLTAFVTKAHLCRAILEATAFQAYDIF
                     KAMESDSGIRTTELKVDGGMTNSEPLMEFQADLLGVPVIRPQVVETTALGAAYAAGLS
                     VGVYKDLDELSSYWKERKRWESTMDDETRSEKVRFWKKAVERTLEWV"
     gene            9295..10116
                     /gene="cdaA"
                     /locus_tag="PLF67_05655"
     CDS             9295..10116
                     /gene="cdaA"
                     /locus_tag="PLF67_05655"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOE89533.1"
                     /translation="MFTDIIQGILAFLRPAVQIGILAWVFYRFYAAIAQTKALALVRI
                     VIVLFSVYALSFLFKLDVLLWFYRYITVPTTILLCVVYQAELRRAFSQLWSGGGRIFR
                     LGTQTTSSDQIDSILNACNVLVNKQRGALIVFPRKLPIRQIIDSGTRLNADLSSSLIL
                     TVFDHDTPLHDGAMVIQGGKILAAGCYLPLSEQTDIKKSFGTRHRAALGLAEESDAVV
                     LIVSEETGAISLTYNANLYYDLSIPTVKRMLLALFSYHDITPEEVMKEAGSEEVE"
     gene            10100..10507
                     /locus_tag="PLF67_05660"
     CDS             10100..10507
                     /locus_tag="PLF67_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOE89534.1"
                     /translation="MKRLNKYLQGFLVNWPAKVLSLLLAVAVYAFIHYSTIGARSVTI
                     PITVLLPERMEAQSLVPDSIDVEIRGDEHIIYLINPDSITAVVDFSKVEKEGITTANV
                     ILTHDREVFERSEITLKASPSSVRVLFGPRSGP"
     gene            10504..10866
                     /gene="acpS"
                     /locus_tag="PLF67_05665"
     CDS             10504..10866
                     /gene="acpS"
                     /locus_tag="PLF67_05665"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOE89535.1"
                     /translation="MIGIDVVAIRRIEDLSEHARKRIFHPEELAQAERSPRPGEFLAG
                     RFAVKEAFFKAVGNGMKTLSTQDVWTTRGEHGEPVINLSSKARALTGGRTVTLSISHD
                     DPVAVAVVLISGGNDGAL"
     gene            10853..11701
                     /gene="truA"
                     /locus_tag="PLF67_05670"
     CDS             10853..11701
                     /gene="truA"
                     /locus_tag="PLF67_05670"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOE89536.1"
                     /translation="MARSDWKRPALVPIEAGKRRIKMILSYDGTPYSGWAYQTNAPTV
                     VGVLQQTILEMIDEEVEVIGSGRTDSGVHAAAQVCHFDIAHRNIKSGKFKPALNRLLP
                     QSIRILHSEEVDGSFHARYTTMGRTYRYYFKREGELLGFDEHRVAKLKAFPSLPLLDG
                     YAKILVGTHDFTTFAAASDNSESKVRDIYESYWKIEKDRFGAELLTYTISGNAFLHKM
                     IRSLVGSMIQFAGQKMGVEEFVGILEAKDRFRAGRTAQACGLYLYRITYDEEEYAWFE
                     EEYGGH"
     gene            11691..12416
                     /locus_tag="PLF67_05675"
     CDS             11691..12416
                     /locus_tag="PLF67_05675"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOE89537.1"
                     /translation="MDIEDSIRTFVTICDEAEALISDREVDPTSTGDLSPVLDLFITP
                     SFDKNRVDQMSFKHLEEAVRGCSLCRLAETRTKTVFGTGVPSPTLMVIGEGPGGDEDL
                     QGEAFVGRAGKYLDAWLNAINLSRTNGVYITNIVKCRPPNNRDPQGDEVEACVPYLSR
                     QIQLLQPQAILLLGRVAAQTLLGRKDALAALRGTFHRYEGIPVLVTYHPAAVLRDQRL
                     KRPVWEDLKKIASFLNIALPGRS"
     gene            12416..14380
                     /gene="priA"
                     /locus_tag="PLF67_05680"
     CDS             12416..14380
                     /gene="priA"
                     /locus_tag="PLF67_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOE89538.1"
                     /translation="MALYADVLVDVPLKQSFTYLVPEGLQEASLFGCRAVVPFGSREV
                     TGYIVGVRDTDDSPFELKELKRMIDSEPVFTEKTVELVRWISRFYLCSEGEAISLVIP
                     GGRRDSAYGGLEQDEAFSAGRIERLSAEQEAALSNILNSDERMFYLHGVTGSGKSEVY
                     LRTAEAVIAEGKGVIYLVPEITLTHQLAALVAGRFSNRVAILHSALTPSQRLKQWRRI
                     LNGEVDLVIGARSAVFAPVENLGLIIIDEEHENSYKSGSTPRYHARQVAQHRVRKEGA
                     RLIMGSATPSLEAYHLMHKTRAITPLFLTKRVAGGAMPKIEVVNLLGEKRTISGRLAR
                     LMKETLAAKKQVILFLNRRGFSYFFHCRSCGFELRCPHCDVSLTYHKHKGRLVCHYCG
                     YARNPITSCPSCHSLDVGYSGYGTEMVEEEVKSLFPFARLERLDTDTAQKKGHIQAVL
                     ERFRNGETDILLGTQMVAKGLNFPHVELVGIVLADSGMHIPDFRAQERTFSLLVQVSG
                     RAGRYTDKGRVVIQTFHPDNPAIVHALSGDLASFYEGELIARKETNFPPYTRLINITL
                     RGRNRERVTKEAGKLAALFNQASGTKGPGLLVAGECPVEKIASNWRYHLLVSGESAAQ
                     AHHLVSTVLGSYTPSGSVYVEVDIDPLTLM"
     gene            14430..14933
                     /gene="def"
                     /locus_tag="PLF67_05685"
     CDS             14430..14933
                     /gene="def"
                     /locus_tag="PLF67_05685"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOE89539.1"
                     /translation="MLDIYTLGEEVLTEKAKPVKTFDSALALLVDAMFETMAEADGVG
                     LAAPQVGVSERLFVIDVRDGKRWVFINPEIIATSEECVTAEEGCLSIPGVFSEVTRAA
                     RVTVQARNLQGRPFTVEAEGLFARAIQHELDHLNGVLFIDRLDKDEHDRAVQAFNRRN
                     RKRRTRA"
     gene            14930..15862
                     /gene="fmt"
                     /locus_tag="PLF67_05690"
     CDS             14930..15862
                     /gene="fmt"
                     /locus_tag="PLF67_05690"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOE89540.1"
                     /translation="MRILFAGSSEIAVPTLKAVAVRYEVGAVLTNEPKPGKRGRTPIP
                     TPVEEAAFELGLPVLRFDRLGKDARRAVAIHECDTLLCFAYGRIFGPKFLNLFSGEVL
                     NIHPSLLPEFRGPTPIQGAILHQKTESGITLQKLDLKMDEGDILLAKRFPLAGDETAR
                     SLTEYVAGEAALLAIEGLNRIMEGTALFTPQTGRATYTPLLTLDHAPLDFSKDARSLH
                     AQIRALNPWPKARCTYEGQSLIISGVWGRVNELGFEVVEPGRVLGFDKEKGIGIGTGL
                     GVLWVTTLQLEKRKEMDWRSFINGNQRFIGSRLE"
     gene            15865..18054
                     /locus_tag="PLF67_05695"
     CDS             15865..18054
                     /locus_tag="PLF67_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE89541.1"
                     /translation="MKRFAALLSIVFLSTSLLFAEEPTVALVLSGGGARGMAHIAVLE
                     ALEEEGIPIDLIVGTSIGALIGGMYSVGYTPNEIREILFNLDMIELFSEAVTEEGGTL
                     DRAFVPDRNHVFNLRFSGSSIGKTPAIIGDQRILELLGFLFAKYPNPIDFDELPIPFR
                     AISSDIMSGDEIVHDFGSLVRAIRASISIPLIFAPFPLEDGRLAVDGGAVDNLPINVA
                     RALGADYVIASDLNAFINEDKNQAETLSGVVIHSLSLVIQRSAFAQHEAADLLFTSDL
                     HDISIMDAFKLEAIEQRGREAVERNRPQLQALRNRLKTERDLIVRDPHRVGSYALKPD
                     PIIRSVAIENISLTPKPLFFDESLFTFMVGSPLDKEHAAELALRLRQVRKQNSLASLS
                     YEMKEGGTLGILARSFGQTGNHIAMGFIADMGVSNTLNDTYVWFKANAYLDAALVHVG
                     RNNLSYTLAASLGEQTAVTIGLIVPLENHRLGALIGYGSGALTPQASVVNAARSAPLD
                     RKIRSEFSYGYTIDKQASLELALRGDFIFIHDSILGKPFVAYPHAELSCVYSTLKKRL
                     AQEGVRFESSFVAGYDKRFLFSGRLRFHHHIRLSFFDTLGYFIEAARLDGPRELIESF
                     GEVAIPGYAPHMLKRDMIQIGTHYQRYLFDVFSYPSYLRFDIVGALFNQANPIVELLP
                     ARTGFMDLEAFDLGFGASFGVQTPAGDLLFSIGVSIDGAWSISMGIS"
     gene            18027..18992
                     /locus_tag="PLF67_05700"
     CDS             18027..18992
                     /locus_tag="PLF67_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607951.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOE89542.1"
                     /translation="MEHLNGYLLMMERYNTYGRYLQERYGHPVYRIGVDAHFTCPNRI
                     GGLGGCVFCDEAASKAVYQEGVGEDARDLEGRIRSVSKQIARGKEFIKRRYGSEHFSL
                     YYQSHTNTYDSIENLKKIYQSGLDEGPFVELIVSTRPDCITEEIVELLASFKDRVEAV
                     VVELGLESSDEGSLVRLGRNHDVASYRRSVGLLRSVGIGVYTHIMLGLPKEDSSTVVK
                     TAHLLNEMGTDGVKIHDLHVLRSTVLEDWHESGEVTVASSDRHLASLVTFLRHLEPRI
                     VVARLICETPRSRRIAPRFPIPKQQIIARLSALLEEGGYVQGDLL"
     gene            18989..19483
                     /locus_tag="PLF67_05705"
     CDS             18989..19483
                     /locus_tag="PLF67_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic resistance protein VanZ"
                     /protein_id="HOE89543.1"
                     /translation="MTTSKPERIIRRIGTVLMIIVVCCIGYFSFSNRTGGISLGWVPF
                     GDKGLHFLVYAAYGFSSFLAFFRAPEEETAKRREGSVVHFSTWASKAILRTLVSGTLL
                     GGIIELLQPLAGRQREMMDLVANVMGLIVGLAIILPVVKTIGRMPWFLIDKEDDEYEL
                     GETD"
     gene            19461..20318
                     /locus_tag="PLF67_05710"
     CDS             19461..20318
                     /locus_tag="PLF67_05710"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOE89544.1"
                     /translation="MSLAKRIEKALIDAARILHEPIHKRLEWAREEEERLDGKSVGSK
                     ASIAVLEAILENLRISRNEWIPMCQDTGMFDVFVDVGRSCPLSHAEIEEAILTGCEEG
                     AKNGYFRPSVVEEPLFGRKNTKTNTPPIIWWNLVEGRNVEINILLKGFGSENTCATRM
                     MNPTATEEQIVAAVAEIVTIAGGKPCPPIFLGIGLGGTMERAAYLSKRALLREVGVPH
                     SVERYAKLEEKILAGVQATGIGAGGLGGKITALNVAVEYEPTHIAGLPMGVSINCWAD
                     RIAHIVWEG"
     gene            20321..20839
                     /locus_tag="PLF67_05715"
     CDS             20321..20839
                     /locus_tag="PLF67_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOE89545.1"
                     /translation="MRTLHLPISEEDLQSLRAYDAVSFSGTLYVGRDQVHQRLARLLE
                     EGKPLPVELEGQAIYYMGPSPPLPGMIMGSCGPTTSARMDPFTPQLLGKGLKVLIGKG
                     PRSKAVHDALKKEKAVYVQAFGGCGALYAKTIVSVETVAFEDLGPEALLRLEVRDFPA
                     IVAIDSHGGSVF"
     gene            20981..21760
                     /locus_tag="PLF67_05720"
     CDS             20981..21760
                     /locus_tag="PLF67_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HOE89546.1"
                     /translation="MLRKIIFAITPVSLIFLLAGCPILIEPSVLDDVLPSVAQMISDE
                     GGDLYTFSVSDLPTPSTLEERFSYTYGYMLYNTLLQQDEIANLNAPYFAKGALDANAG
                     SAIFTADEMNRILFEVQLMLLQIAQQELEALSKANYEYAHAFLEENKTREGIITTESG
                     LQYTVIEPSDGPHMDPSLSPRVRFTLTDGQGEVVDSAESRLLSLGNLTPGFIEGLLLM
                     NVGGRYRFWLAPDLTYPDGGESNPAGPNALLIADVTLLGLE"
     gene            complement(21725..22231)
                     /locus_tag="PLF67_05725"
     CDS             complement(21725..22231)
                     /locus_tag="PLF67_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89547.1"
                     /translation="MKRIVLLLVLLIASTLLFAGEPVKGIGAGISTGIPFTVGALGEY
                     NFGPAYASVELGYATGPYFMFRAGGGYNFPKPFVNSDLGMDLYLSIGGKFSGYLGSFS
                     GNFVAVGAFGIPVTWSWYANKIPIKVFAQAGPEIYFGSGSVDLNFFGSIGAMYVFSLE
                     PKQSNVGN"
     gene            complement(22359..23045)
                     /locus_tag="PLF67_05730"
     CDS             complement(22359..23045)
                     /locus_tag="PLF67_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOE89548.1"
                     /translation="MYHHLFLDLDGTLMDFREAERRAFTRMAGQLRIPIDDASHLLYA
                     RCNHSCWKEFEQGLLTIDELSLKRFVLFQEQSNLRFDVESASEVYEDHLSRQAILYPE
                     TLPLLTLLKDEGYRLHLASNGISRVQRGRLEASNLDRFFDHIFISTEIGVPKPDPGFF
                     EHMLRTTEAGKHECVMIGDSLSSDIQGALDVQLDCIWLNTENRETTLEPTYTITSLSE
                     LHPLLDSIKL"
BASE COUNT         4750 a         6533 c         6902 g         4883 t
ORIGIN      
        1 tacacggtcg acggaggcag gcgcttcgag atccagagcg aatgcttcaa cgccgtgacg
       61 gtgaaggttg gcttcgaagg ggtgagctac catctcggag cggcccgggg ccggatggtc
      121 aacgcactta cgatggcgag cgcgttcatc ggcgccctcc cgcaggcgga gagccccgag
      181 gcgaccgacg gacgatacgg ctactattgt gcccatacga tcgaaggctc gatccggcac
      241 gtcgacctga ccgtgtatct gcgggacttc gaccttgaaa acctcgatcg gcggatcgag
      301 gtgctgaagg gtctcgcccg gacgatcgaa ggcctctatc ccggtggaaa ggtcgatctt
      361 caggcgaagc acatctacta caacatggct cagatcgcca aggataaacc gattgctctg
      421 gacaccctct tcgctgcggg gaaggcgctg ggctttgagt tgaagaacga ggtgatccgc
      481 ggcggaacgg atggatcccg catggcgaac gaagcgggga ttccctgccc gaacatcttc
      541 accggcggat acaacgtcca ctcccgctat gaatgggccg cccttccggc gatggtcgat
      601 gcgacccgcc tgatcctcaa gatcatcgaa ctcggagccg cattgtgaag cgaatcggga
      661 ctctggttct tctgctcttg gtaaactcgc tgatcttttc ggcgattcac gttcaaaggg
      721 ggccgacgat tcccggtgcg ttgatcgacc acacggaaga agccctcaac acggtgatgg
      781 gcccgcgtct caaagagaat caagagttga ggggtttcct caccgaagag gcggaccggt
      841 atctcctgac ggttaacctt gatggccggg agctgatgat tccgattccc ctttcatggg
      901 aggcggagcc gagctatatc tccgactttc tggtctacga tggcctcgcc ctgcttgacg
      961 atcttccccc gttgatgatc gatgtcatct cgaagacaag cgttcttgtt gaaaaccccg
     1021 atccttcggt tcgcgtcggc gatcgcttct gggcgatcga cgcccgggga aacaggatcg
     1081 gcctgcttgg cgtccgaagg gtcgacgaaa gccggcttct tttgcagcag gagggcggaa
     1141 agacccttca ggttgggatg gggctcgtgc agggaccgga gataccggta tatctctcca
     1201 tgagcatcga tgctcaacgg gtctttgggt tccatgcagg tgcatgcatt cctcttcgtc
     1261 tctatccatt ctccctcgcc gttgagggag gcaccttggg cggcagggag ttttcgctgg
     1321 gagccggggt aaaagctcgg gtgagctttt cccagctctt cggaaccggc tggactcctt
     1381 ttcgggcgct gagtttcagt gccgctgttt cgggaggagg agcctactcc cttggcaggg
     1441 ggtggggagg gtatgttcag ggaacctttc ttctggagta cgccctcggt cgttggatca
     1501 tcttcgccgg aggcggagag cggatgcggg cattcggatc atcggtgcat gatggactgt
     1561 tctttgtcgc aggaacggcg tacactttct agcgtatgaa gaagctgctt cccttgatga
     1621 tcgtcattcc tcttctgctt gcctcctgcc gcttggcgaa ggatctgccg ggttcaggaa
     1681 cgtcttcccg ggcgagtcct cttgaaggaa gggcgagcca ggtgttcttc cacggcgagg
     1741 gacggggcct tgagccctat caaaccctct ttgcccggat tgaagcatcg atcgggttcg
     1801 acctcgatca gcgatacctt gacgagctca tcgataccgg aggcattgcg gatctcggct
     1861 tggtgatcac caaccaatat cggaaagggg acgagatctt tctccgggcc caggcgaacg
     1921 aagaggtgat cgaacgctgg cgaacggagc ttgcccaagc gcttcaggat cggatcgacc
     1981 ggatcgagga gctgctccgg gaagcgaatc tcgcctacaa agccaacgat gacacgataa
     2041 ccatcacccg atacctggag gcggcgttgg tcgcctccgg cggtccggtc ggagagaaac
     2101 gctatgaacc ggaagcgctg gtcaccgaag cgatccgcta catcgagccg ctgcagttcg
     2161 tcctgacccg cagcgaaagc gcctccgccc gaacgacggt aacccttcga agacggacgc
     2221 ggctgctggc cccccgggtg atgcatgccc cgatttccgt cacgtacgaa gcgtacaacg
     2281 cccttggcgc cctctatgag gataccctct acttcaacag tcacgattcg gggcagctcc
     2341 tcttcaaccc gctgaacgcg gcgatgaaaa gcgagggagt catcaccttc gcgctgaatc
     2401 tcgacattcc tccgttgcct cgggatcttg ccctccgcgt cgaagaagcg ctgaaaaagg
     2461 tctcgatcga gtttccgtac tccctttccc atccattcgg accggaatcg atcgggcttt
     2521 cgctcgagga gtattcgatc agcggcaatc ttttgcctac cacccatgcc aaaaccgcct
     2581 tcagtgaagg ctcgaaacgg gacgggatcc ccatcttgga cgtcgcactg gagcgggcgg
     2641 cggaaaacga gcttgaagat ctggttcgtc agattcccaa gaatgtccgc tacacgatca
     2701 tcggctcggt gggcgtgctg agcttcgaca aagccttgga cgatgtgatc gccgtggtca
     2761 gcggctcgtt catcctctac gactcggtcc gttcggcgat cctctatcat tcccaggata
     2821 ttgaagcggt gggatgggga gcgacccggg aagaagcaac ggctcgcggg ttcgagcgct
     2881 tcggctcgat cgccgaatac ctgcttcgga ctgttttgat ggatgtgcgc taggatagaa
     2941 gcgctctttt gtgctatagt ctcagcatga cgttacgcga gatcttagag cttaccgaag
     3001 ccacccttgt ttgtggaagt caccatgccg acgatacgat cacctatggg tttgccagtg
     3061 acttgatgag cgatgtgctc accatcctga aagatgatat catcatgatc accggcctgt
     3121 cgaatatgca ggcagttcga accgccgaga tgagcgatat ccgcaacatt ctcctcgttc
     3181 gaaacaagca gcccagccag gcgatgatcg agatcgccga agaactgggc atcagtatct
     3241 cctacacccc gtactccctc ttcagagtga gcgggatcct gtatggcgcc ggtttgaaac
     3301 ccgtctatta aaggggaagg cgccggcttt ccacgttgag aatttccagc ttcttcgcct
     3361 ctttccccag cagttcgccg cttgccagga cgaccagatt gctcccgtcc cgctccgctt
     3421 gttcaagcag cgctttcgct ccctcattga ggtcttcgtt tgagagctca agaagctggg
     3481 ttcgccgcat caggcggaac tcgtcggtga tcgagtagag aatcctgcgg aatcccagaa
     3541 gcgcatcctg gccgggtgaa agcggcctga gctcccgtcc gacgatgaag atcgtgctgt
     3601 cttcaatctc tttttcactg aaaacacggg tcgcggcttc ctcaagcacc ctcttcaggt
     3661 cggtgaacgt tccggcgatc ctcggatccc gataggtgga aagaatgaac gtctcctcgg
     3721 ccacatcggc actggcggaa accccgtacg ctccgccttg cccccggatc gtttcccaaa
     3781 ggggccccgt ggtaagaatc tgcgagagga gaacctgagc ggattggagc acgcttcccc
     3841 gcttggcgga gcgaagcgcc caggcgctgt agctgaccgt cgacggcagc ttgaagagat
     3901 cgacgaccgg gtttgaagtc ggtccgacct tctcgtagct cctcggctct ttgtcaagcg
     3961 gtccccggtc ttcgaagcgg gtgatgaaca tctcgaagct ctttaccgaa tcttccagca
     4021 aaggctcctc gcagcttaga tgggcgacca gccttcggcg gttcgccagc ttcgtctgca
     4081 gggctttgag ccgcccgccg aggctgtcgg cttcaagccc ggtaagatgg aaccactggg
     4141 aaagcccgcc gatcagctcg gcgtcccacg ccgcctcgct gaaggttcgg gtggcgtagc
     4201 tcgaggcgaa attcgttgcg ccataggtga cgacctcgct gaactcgctc ttgtagttgt
     4261 tgaggacgaa gcgcagctgt ttcctgtcat ccacattcgc tccaacaagg agctcctgaa
     4321 tgaagcccag cgcctcatcg aaatcctccg ccagggtctt gattcgaacg atcagaaggt
     4381 gaaccgcctc tccttgaagg gaagttccca cctcgacgaa gaaggagaag ccgccggtca
     4441 accgtttgat ccgctgcgcc accgtctcat aaggcagctc gccaaggccg gtggtctgaa
     4501 ccagacgggt gtagaggttg agaaggagca tctcctcttc agtcaaatca tcaatcgtga
     4561 aggcgatgtc ggcgtagacg atcttgttgg tgggaatcgt gcggatccag agctccctgc
     4621 cggcaatgat cttcagagca tggtcgttcg gccggatctc tttcggcaga tcctccaacc
     4681 ggaggaaggg gatcttctcc agattctccg tgctgtcccg ctcctcctcg aaggcggcaa
     4741 agcgcctgtt ctgctcgatg atctccttca tcccgtttct gcccggctgt ttagcggcgt
     4801 ggagcgcccg cttggcgatc gcttcgtttt ggcgcctctg atgctcttca tccggcttca
     4861 ccgtgacaag gatccgatgg gggttgtcaa ggaggtgatc cttgatccaa tcctcgaaat
     4921 accgggagtt ccgcttgatc cgtttttcca gctcgttgag ggtcggaacc aactcgatcg
     4981 tctccgaggg cgaccggtcg accatccacg cccgggtggc tcgggtcaat gccctgaggc
     5041 cggtgggaat accgccggga atctccagct ggctgaagcg ggtgcgcttc agcgacgttt
     5101 cgatgagctc gggatcgaga ccgtcccgga cgatcttttt cagcgtggaa agaatgacgt
     5161 tctgcgcctt ttcaccgtct tccgggtcga tccccttgaa gccggcgatg aagggcatct
     5221 gccggaactc ggcgctcatc ccgctctcgg gcgagatgtc cgaggcgaga tccgaatcga
     5281 tgattgcttt gtaaagagga gcggcggggt tgccaaggag gatgtccacc aacgtggaaa
     5341 gggtcatcac cgagagcgaa tcgtcgttct ccgtggtcgc ccaggaaaga atgatcgagg
     5401 cgttcttcac ctcttctccg ccttcctcca tcgggctggt gaaggtgacc gcccgctcgc
     5461 ggtcccaccg ccgggcaagc ggaacgatcg agtcgatctg aagcattccc ctcgtccgga
     5521 ggtagtgctc atccagatac gccagctttt ctccgacttc catccggccg aagagaaaga
     5581 gcttcgcatt ggaggggtgg taatactgat cgtagaaaga gcggaactcc tgataggaga
     5641 ggttgatgat cgcttcggga tcgcctccgg attcgaagga gtaggggttg tcgggaaaga
     5701 gcgcctgcac cgccgaccgg ctgacgatcg catcatggtc ggcgctgacg cccagcatct
     5761 cgttgaagac caccccttcg tagtggcacc cctcctcgtt gcagacgagg cgcaccccct
     5821 cccgccagaa cgtctcctcc cggagcaggg gggcgaagac cgcatcggtg tagacggcaa
     5881 aaagattgtc gaagtccttc ttcaggggac tggcggccgg gtacacggtc cggtcgggat
     5941 aggtgagggc gttcatgaag gtattggcgc tgcctttgag cagcgtcatg aagggatcgt
     6001 gcaccggata gcgctccgat cccgccagga ggcagtgctc gatgatgtgg gctacgccgg
     6061 tatcattgca gggaagggtt cgaaaaagat agccgaagaa gcattcgggg tcttcgttga
     6121 ccacctggta gacttccatc tttgtttcaa tatgtcgaaa gaggtacccg gttccctgat
     6181 attccgccaa gggggtgatc tcttccaaaa tgaatccgta aagctcgtcg cctgcggcga
     6241 gctgtcgttc agccatggat gacctccgtt acaggacact aacacgcctc aaatcaattg
     6301 acaagagcgc cggaaacacc cgatcggagc atcatcacat ttgccgggca ggttaccacc
     6361 ccatctcctc aagccaccgg acggttcttt tctcacgttc cttcgcttca tcgtggacat
     6421 attttccaag cctcagttca cctttgatct cgccgtccag catatagagg atccgatcac
     6481 acaccgcggc gttcctgctg tcgtgggtga ccatcatgat tgaggttcct ttcaggttga
     6541 tctgtaaaaa cgcctcgatc acgtcggcgg ccgcctgatt cagcgctccg gtgggttcgt
     6601 ccgcgaagat gatctcgggc tccatcatca gactgcggca gatgcacgcc cgctgcagct
     6661 gcccgccgga cacctcgttg accctccgct cggcaagatc cccgatgtgg aaatcatcca
     6721 tcagggcccg ggcgcgccgg tagagttccc gcttccggct cttcttcgca tggagcgccg
     6781 gcagcacgac gttgtcgatc atgttcaaat tcgccggtac gttcatctgc tgaaatacga
     6841 agcccatcct accggtgcaa atcgattgac aagaggtggg ctgagtccta ccgtggaacc
     6901 atgtactata tcggagccca cacctcgatc gccggcggac tggaaaacgc cctcttggag
     6961 gcgcagcgct tgaacgcaac cgccttgggg atgtttacga agaatcagcg cagatgggcg
     7021 gccaaaccga tcgaagcgga agaggcggat cgatttacgg aggtgttgca ggaaaccggc
     7081 ttcggttccg accggattct gatccacgga agctacctga tcaatctcgg caaccccgat
     7141 cgggagaaac aacgacgttc gattcgagcg atggccgatg aacttgggcg cgccgagcgg
     7201 ctgggtctga agttggtgaa cacccacccc ggctccacgc tgggcctcgt ctccaaggag
     7261 gaggggatga agatcgtcgc tcggggaatc gatgaggcgc tcgatgagag ctcgaaggcg
     7321 atcctcgtca ttgagaacac cgccggcagc ggttcaaacc tcggctcgac aatcgaggag
     7381 cttgccggaa ttttggaagc gacgaggaac catgcccgcc tggggttctg cctggatacc
     7441 tgccacgccc acgtagccgg ctacgacctt tcgacggtcg aggggttcga gaacctgatt
     7501 gaccgctttg atcggttgat cggcctcgaa tttctgcgag gtcttcacct caacgatgct
     7561 ttaagcgaaa gcggcagcaa acgcgaccgt catgccagcc tgggcgaagg gacgatcgga
     7621 tggccgacgt tcgagcatat tgtccgggat ccccgcttca gaggaatccc gctggttctt
     7681 gaaacacccg atccctcgat ctggggcgat gagatcgccc atctgcgcgc tttgactttc
     7741 aaaaaccggg tgtgcgaagt atactggccc aagaggagga acggatgaag tatttaggtg
     7801 cagtagacca agggacgacc agtacccgat tcatcatctt cgatcagggg ggtgcgatcg
     7861 tcgcttccca ccagcttgag cacacgcaga tttttcccca gccggggtgg gtcgagcacg
     7921 acccgatgga aatctgggcc aatacgcaga agtgcatcac cgaagccctg aaaaaagccc
     7981 gcctgagggg cagggatctt tccggaatcg gaatcaccaa ccagcgcgag acgatcgtgg
     8041 cgtgggaaaa ggagagcgga cgggtctacc ataatgcaat cgtctggcag gaccttcgga
     8101 ccagcgacca tatcgaggcg ttgaaggaca gcccgaacgg cccggtcatc gaacaaaaaa
     8161 gcggactggt cctcagcccc tacttcggcg ggtcgaagat cgcctggctg ttggagcatg
     8221 tcgaagggtt gcggtcggac gccgaatcgg gcaaagcggt cttcggaacg atcgattcct
     8281 ggcttgccta ccaactttcc ggcaaaagga tccacgtcac cgatgccagc aacgcgtcgc
     8341 gctacctctt gatgaacatc gagacgctta cttgggatga tgagctgctt gaaatctttt
     8401 ccgttccgaa atgctctttg ccgacgatcg tgccgtccag cgggatggtg tacggccata
     8461 ccgtcaagga aggccccctc ggagccgaag tcccgatcgc cggaatgctc ggcgaccagc
     8521 aggccgccct cttcggccag gcgtgcttca agcccgggat gggcaagtcg acctacggca
     8581 cgggctgctt cctgctggtg aatacgggca ccggtctcgc ccggtcgcaa aacggcctgc
     8641 ttaccaccgt cgcgtacaag atcggtgatg aaaagccggt gtatgcgctt gaagggtcgg
     8701 tagccgtggc cggctcgctt gtccagtggg cgcgcgacaa cctgaaactg gttgaaagcg
     8761 cccagcagct ggaccgcgaa gcgcttacgg taacggactc gggcggagtc tatgtggttc
     8821 ccgcgttcag cgggttgttc gccccctatt ggcggagcga cgcccgggga gtgatcgccg
     8881 gcttgacggc gttcgtcacc aaagcccacc tctgccgggc gatcctggag gccacggcgt
     8941 tccaagcgta tgatatcttc aaagcgatgg agagcgacag cggcatcaga acgacggaac
     9001 tgaaggtcga cggaggaatg acgaacagcg agccgctgat ggagttccag gcggatctgc
     9061 tcggcgtgcc ggtcatccgg ccccaagtcg tcgagaccac cgccttgggg gctgcatatg
     9121 ccgcaggact ctccgtcgga gtatacaagg acctcgatga gctttcctcc tactggaaag
     9181 aaaggaagcg atgggaatcg acgatggatg atgaaacccg gagtgaaaaa gtccgatttt
     9241 ggaaaaaagc ggtcgagcga acgcttgagt gggtgtagga aaggagcggt ctagatgttc
     9301 accgatatca ttcagggcat cctcgcattc ctccggcctg cggtgcagat cggaatcctt
     9361 gcatgggtgt tctatcgctt ctacgcggcg atcgctcaaa ccaaggcgct tgcgctcgtc
     9421 cggatcgtga tcgtcctctt ctccgtctac gcgctgagct tcctcttcaa gctggatgtg
     9481 cttctgtggt tctatcggta catcacggtt ccgacgacga ttctgctttg cgtggtctat
     9541 caggcggagc tgaggcgggc attcagccag ctgtggagcg gcggcggccg gattttccgg
     9601 cttggaaccc agacgacgag cagcgaccag atcgattcga tcctcaatgc atgcaacgtg
     9661 ctggtgaaca aacagcgcgg ggcgttgatc gtctttcccc gaaagcttcc gatccgccag
     9721 atcatcgaca gcgggacccg cctcaatgcg gatctttcct cctccctgat cctcaccgtg
     9781 ttcgatcatg acacgccgct ccatgacggg gcgatggtga tccagggcgg caagattctc
     9841 gccgccggct gctacttgcc gttgagtgag cagacggata tcaagaaatc cttcgggacc
     9901 cgtcaccggg cggccctcgg ccttgccgag gaaagcgatg cggtcgtgct gatcgtcagc
     9961 gaagagaccg gggcgatcag cctgacctac aacgctaacc tctactatga cctttcgatt
    10021 ccgacggtca agcggatgct gctggcgctc ttcagttatc atgacatcac cccggaagag
    10081 gtgatgaagg aggcgggaag tgaagaggtt gaataagtac cttcagggat tcctggtgaa
    10141 ctggcccgca aaagtccttt cattgctcct tgcggtagcg gtctatgcgt tcatccatta
    10201 ctcgacgatc ggggcccgca gcgttacgat tcccatcacc gtgctgctcc ccgaacggat
    10261 ggaggcgcaa tcccttgtgc ctgattcgat cgatgtggaa atccgcggcg atgaacacat
    10321 catctacctg atcaatccgg attcgatcac cgccgtcgtc gatttctcca aggtggagaa
    10381 ggaggggatc acaacggcga acgtcattct cacccatgac cgggaggtgt ttgagcggag
    10441 cgagatcacc ctcaaggctt cgccaagctc cgttcgggtg ctcttcggtc caaggagcgg
    10501 cccttgatcg gaatcgatgt cgtggcgatc aggcggatcg aggatttgag cgagcatgcc
    10561 agaaagcgga tcttccatcc cgaagagttg gctcaagcgg agaggagccc ccgccccggg
    10621 gagtttctcg ccggtcgctt cgccgtgaag gaggcgtttt tcaaagcggt gggaaacggg
    10681 atgaaaaccc tctccactca ggatgtgtgg accacgaggg gtgaacacgg cgagcccgtc
    10741 atcaacctct cttcaaaagc ccgggcgctc accgggggga gaacggtcac cctttcgatc
    10801 tcccatgacg atccggtggc cgtcgccgtc gttctgattt cgggaggaaa cgatggcgcg
    10861 ctctgattgg aaaagacccg cccttgtgcc gatcgaagcg ggcaaacggc ggattaaaat
    10921 gatcctctcc tatgatggga ccccctacag cgggtgggca tatcagacca atgcgcctac
    10981 ggtggtcggc gtgctccaac aaacaatcct tgagatgatc gacgaggagg tcgaggtgat
    11041 cggcagcggg cggaccgaca gcggggtcca tgccgccgcc caagtctgcc acttcgacat
    11101 cgctcatcgg aatatcaaaa gcggcaagtt caaaccggcg ctgaaccgcc tgcttcccca
    11161 atcgatccgg atccttcact cggaggaggt cgacggctcc ttccacgccc ggtatacgac
    11221 gatggggcgg acctatcgct actacttcaa gcgggaaggg gagctgctgg gcttcgatga
    11281 gcaccgagtg gcgaaattga aggcgtttcc ctcgctgccg cttcttgacg gatatgcaaa
    11341 gatcctcgtc ggaacccacg atttcaccac cttcgccgcg gcaagcgaca acagtgaaag
    11401 caaggtccgg gacatctatg aatcctattg gaagatcgaa aaagaccgct tcggggccga
    11461 gttgctcacc tatacgatat ccggaaacgc ctttctgcat aagatgatcc gttcgctcgt
    11521 cggttcgatg atccagtttg ccggccaaaa gatgggcgtc gaagagtttg ttggaattct
    11581 ggaggccaag gatcgctttc gggcgggccg aaccgcccag gcgtgcggcc tgtacctgta
    11641 ccgcatcacc tacgatgaag aggagtatgc gtggtttgag gaagagtacg gtggacattg
    11701 aagattcgat cagaacattt gtaacgatct gtgatgaagc cgaggcgctc atttccgacc
    11761 gggaggtcga tcccacctcc acaggggatc tttcaccggt tctcgacctc ttcatcactc
    11821 cgagctttga caaaaaccgg gtcgatcaga tgagcttcaa gcacctcgaa gaagcggtca
    11881 ggggatgttc gttgtgccgg cttgctgaaa ccagaaccaa aacggtcttc gggacggggg
    11941 ttccctcccc aacgctgatg gtcatcggcg aaggcccggg gggggatgaa gaccttcagg
    12001 gtgaagcgtt cgtcggtcga gcgggaaagt accttgatgc gtggctcaat gcgatcaacc
    12061 tgagccggac gaacggggtg tacatcacca acatcgtcaa gtgtcgcccg ccgaacaacc
    12121 gcgatcctca aggcgatgag gttgaggcgt gtgttccata tctttcccgc cagattcaat
    12181 tgctccagcc ccaggcgatc ctcctgctcg gcagagtcgc cgcccaaacc cttctggggc
    12241 gaaaggatgc tcttgccgcc ctgaggggaa cgttccaccg ctatgaagga attcccgtcc
    12301 tcgtcaccta ccaccccgcc gcggtcctgc gcgaccagcg cctcaagcgc ccggtgtggg
    12361 aggatctgaa gaagatcgct tcgtttttga acatcgctct gccggggaga tcctaatggc
    12421 gctctatgcc gacgtgctgg tggatgtgcc gctgaagcag tcctttacct atctcgttcc
    12481 cgaggggctt caggaggcgt ccctgttcgg ctgccgggcg gtcgtcccct tcggctcccg
    12541 ggaggtgacg ggatacatcg tcggcgtccg cgacacggac gattctccgt tcgaactcaa
    12601 agaactcaag cggatgatcg actccgaacc ggtcttcacc gagaagaccg tcgagctcgt
    12661 ccggtggatc agccgtttct acctctgctc ggagggggag gcgatctccc tcgtcattcc
    12721 cggcggccgg cgggattccg cctatggcgg gcttgagcag gatgaggcgt tcagcgccgg
    12781 gcggatcgag cggctgagcg ccgaacagga ggccgctctt tcaaacattt tgaactccga
    12841 tgaaaggatg ttctacctgc acggggtgac cggcagcgga aaaagcgaag tctatctcag
    12901 aaccgccgag gcggtcatcg ccgaaggaaa gggggtgatc tacctcgttc ccgaaatcac
    12961 cttgactcac cagctcgccg ctctggtggc cgggcgcttt tcaaaccggg tagcgatcct
    13021 ccactccgcc ctcaccccca gccagcggct gaagcaatgg cgaagaatcc tcaacggtga
    13081 agtggacctc gtgatcgggg cccggagcgc ggtcttcgct ccggttgaaa acctcggcct
    13141 catcatcatc gacgaggagc acgagaacag ctacaaaagc gggagcaccc cccgctacca
    13201 cgcccgccag gtcgcccagc accgggtccg gaaggagggg gcccgcctga tcatgggcag
    13261 cgccaccccc tcgctggagg cgtaccatct gatgcacaag acccgtgcga taactccgct
    13321 ctttctgacg aagcgggtcg ccggcggggc gatgccgaag atcgaggtgg tgaatcttct
    13381 cggggagaag cggacgatca gcggacggct cgcccggttg atgaaggaaa ccttggcagc
    13441 gaagaagcag gtgatcctgt ttctcaaccg ccgggggttc tcctacttct tccactgccg
    13501 gagctgcggg ttcgagctgc gctgccccca ctgcgatgtc tccctgacgt accataagca
    13561 caaaggaagg cttgtctgcc actactgcgg ctatgcccgg aacccgatca caagctgtcc
    13621 ttcctgccat tccctcgatg tgggctacag cgggtatgga accgagatgg tggaggagga
    13681 ggtgaagagc ctcttcccct tcgcccgcct cgagcggctg gataccgaca ccgcccaaaa
    13741 gaaaggccat attcaggcgg tgctggagcg cttcaggaac ggcgagacgg acatccttct
    13801 gggaacgcag atggtcgcca aggggttgaa cttcccccac gtcgaactgg tcggcatcgt
    13861 tctggcggac agcgggatgc acattcccga tttcagggcg caggagcgga ccttcagcct
    13921 gctcgtccag gtctccggtc gggcggggcg gtataccgac aaaggcaggg tggtcattca
    13981 gacgttccat ccggacaacc cggcgatcgt gcatgccctc agcggggatc tcgcctcatt
    14041 ctatgaaggg gagctgatcg cccggaagga gacgaacttt cctccctaca cccgattaat
    14101 caacataacg cttcggggac ggaatcggga aagggtcacc aaggaggcgg ggaagcttgc
    14161 cgctctgttc aaccaggcaa gcggaacgaa ggggcccggg ctgctggtcg ccggcgagtg
    14221 tccggtcgag aagatcgcct cgaactggcg gtaccatctc ctggtcagcg gcgaaagcgc
    14281 cgcccaggcc caccaccttg tttccacggt tctcggttcc tacaccccct ccggatctgt
    14341 ctatgtcgag gtcgacatcg atcccttgac cctgatgtaa gggtatgacg ttgacgctcg
    14401 ccgcagtgcc taggtattat gagagaacga tgttggacat atatacgctg ggcgaagagg
    14461 ttctcaccga gaaggccaag cccgtaaaga catttgacag tgcgctcgcc ctgctggtcg
    14521 atgcgatgtt cgagacgatg gcggaggcgg acggggtggg tctcgccgct ccgcaggtcg
    14581 gtgtttccga gcgcctgttc gtcatcgatg tccgcgatgg aaagcggtgg gtcttcatca
    14641 accccgagat catcgccacc agtgaagagt gcgtgaccgc cgaagagggc tgcctttcga
    14701 ttcccggcgt tttcagcgag gtgacccggg ccgcccgggt gaccgttcag gcccggaatc
    14761 tgcagggcag gccgttcacc gtggaagcgg aagggctctt cgcccgggcg atccagcacg
    14821 agcttgacca cctgaacggc gttctcttca tcgaccgcct cgacaaagat gagcatgacc
    14881 gggcggtgca ggcgttcaat cgacgaaaca ggaagaggag aacccgtgcg tgaggattct
    14941 gtttgccggc agcagcgaga tcgcggttcc caccctcaag gcggtggcgg tccgctatga
    15001 ggtcggtgcg gttctgacga acgagccgaa gccgggcaag cggggccgga ctccgattcc
    15061 cacccccgtc gaagaggccg ccttcgagct tggtcttccc gttctccgat ttgaccgcct
    15121 tggcaaggat gcccgccggg ccgttgcgat ccatgaatgc gacacgctcc tgtgttttgc
    15181 ctacgggcgg atcttcggtc cgaaattcct gaacctgttc agcggcgagg ttttgaacat
    15241 ccatccctcc ctgctgcccg agttccgggg gccgactccg atccaagggg cgattttgca
    15301 tcaaaaaacg gaaagcggaa tcaccctcca gaaactcgac ctcaagatgg acgaaggcga
    15361 catcctgctt gcaaagcgtt ttcctttggc cggcgatgaa accgcccgct cgcttacgga
    15421 gtatgtggcc ggtgaagccg cccttcttgc cattgagggg ttgaaccgca tcatggaggg
    15481 aaccgccttg ttcactcctc agacgggaag ggccacctac accccgctgc ttacgctcga
    15541 tcacgcgcct ttggactttt ccaaggatgc ccgatccctt catgcccaaa tccgggcttt
    15601 gaatccctgg ccgaaagccc gctgcaccta tgagggtcag agtctgatca tttccggggt
    15661 ctggggaaga gtcaacgaac ttggattcga ggtggttgag ccgggaagag ttctcgggtt
    15721 tgataaagaa aaagggatcg ggatcggaac gggtctggga gttctgtggg taacgaccct
    15781 gcagcttgaa aaaagaaagg aaatggattg gcgttcgttc atcaatggca atcagcgttt
    15841 tatcggttcg cgattggagt aggtatgaaa cgatttgccg ctcttctgag catcgtgttt
    15901 ttgtcgactt cgttgctttt cgctgaagaa ccgaccgtcg cgttggtcct ctcgggagga
    15961 ggggcgcggg gaatggccca tatcgccgtt ctggaagccc tcgaggagga ggggattccg
    16021 atcgatctga tcgtcggcac gtcgatcggg gcgctgatcg gagggatgta cagcgtcggg
    16081 tacactccaa atgagattcg agagatcctc ttcaaccttg acatgatcga gctgttcagc
    16141 gaagcggtaa ccgaagaggg cggcaccctc gaccgcgcgt tcgtccccga tcggaaccat
    16201 gtgttcaatc tgcgcttttc cggttcgtcg atcggcaaaa ctccggcgat catcggggat
    16261 cagcggattt tggagctgct cgggttcctc tttgcaaaat atcccaaccc gattgatttt
    16321 gacgagctgc cgattccgtt ccgggcgatt tcctccgata tcatgagcgg ggatgagatc
    16381 gtccacgact ttggctccct ggtccgggcg atccgggcga gcatctcgat tcccctcatc
    16441 ttcgcaccgt ttcctttgga ggacgggcgg cttgccgtcg acggcggagc ggtggacaac
    16501 ctgccgatca acgtcgcccg ggcgctcggg gcggattacg tgatcgccag cgatctcaac
    16561 gcgttcatca acgaggacaa aaaccaggct gaaacgcttt cgggggtggt gatccactcg
    16621 ctttccctag tgattcagcg cagcgcattc gcccagcatg aagctgccga cctcttgttc
    16681 acgagcgatc ttcacgatat ctccatcatg gatgcgttca agctggaggc gatcgagcag
    16741 cggggcaggg aggcggtgga gcgaaaccgt cctcagcttc aagcgctgcg aaaccggctg
    16801 aaaacggagc gcgatttgat tgtccgggat ccgcatcggg tcggctcgta cgccctgaag
    16861 ccggatccga tcatccgctc cgtcgcaatc gaaaatatct ccctgactcc caagccgctc
    16921 ttcttcgatg agtcgctctt cacttttatg gtcggttctc cgcttgacaa ggagcatgcg
    16981 gcggagttgg cgctccgcct gcggcaggtt cgaaagcaga attctttagc ttccttgagc
    17041 tatgagatga aggagggggg aacccttggg attcttgccc ggagctttgg acagacggga
    17101 aaccatatcg caatgggctt cattgccgac atgggtgttt ccaataccct caacgacacc
    17161 tatgtgtggt tcaaggccaa cgcctacctt gatgcggcgt tggtccatgt cggtcggaac
    17221 aacctgtcgt atacgcttgc cgcttccttg ggtgaacaga cggcggtcac gatcggcttg
    17281 atcgttccgc tggaaaacca tcggctggga gcgctgatcg gatacggttc cggcgctctg
    17341 acgccccaag cctccgtggt caacgccgcc cgctccgctc ccttggatcg gaagattcgc
    17401 agtgagtttt cgtatggata cacgatcgac aaacaagcat ctctggagct tgctctccgg
    17461 ggagacttca tcttcatcca cgattccatc ctcggaaaac cctttgtcgc ctatccgcat
    17521 gccgagcttt cctgtgtata ttccacgctg aaaaagcggt tggcccaaga gggggtccgg
    17581 tttgagtcat cttttgtcgc cgggtacgac aaacggttcc ttttcagcgg tcgcttgcgg
    17641 tttcatcacc acatccggct ctcctttttt gatacgctcg gctatttcat tgaggccgcc
    17701 cgtcttgatg gaccgcggga actgatcgaa tcgtttggcg aggtggccat tcccggctac
    17761 gctccgcata tgctcaagcg ggacatgatc cagatcggga cgcactacca gcgctacctg
    17821 ttcgatgtgt tcagctatcc cagctacctt cgcttcgata ttgtcggcgc cttgttcaac
    17881 caggcgaatc cgatcgttga actcctgccc gctcgaaccg gtttcatgga tcttgaagcg
    17941 ttcgatctcg gtttcggagc gagcttcggc gtccagactc ccgccggtga tcttctcttc
    18001 tccatcggtg tttcgatcga tggggcgtgg agcatctcaa tgggtatctc ctgatgatgg
    18061 agcggtacaa cacctacggc aggtacttgc aggagcgata cggccatccg gtgtatcgga
    18121 tcggggttga cgcccacttc acctgtccga accggattgg gggccttggc gggtgcgtct
    18181 tctgtgacga ggccgcttca aaagcggtct atcaggaggg ggtgggcgaa gacgcccggg
    18241 atctggaagg acgaatacgt tccgtctcca agcagatcgc ccggggcaag gagttcatca
    18301 agaggcgata tgggagtgaa catttcagtc tatactatca atcccacacc aatacgtacg
    18361 attcgatcga gaacctgaag aaaatctatc aaagcggatt ggatgaagga ccgttcgtcg
    18421 agctgatcgt ctccacccgt cccgactgca tcacagagga gattgtcgag ctccttgcct
    18481 ctttcaaaga ccgggtggag gcggtcgtcg tcgagctcgg acttgagtcg agcgatgagg
    18541 gttcgctcgt ccggcttgga agaaaccatg atgtggcgtc atatcgaagg agcgtcgggt
    18601 tgttacgttc ggtgggaatc ggtgtttata cacatataat gcttgggctc ccgaaagagg
    18661 attcttcgac agtggtaaag accgcccatc tgttgaacga gatggggacg gatggagtga
    18721 aaattcacga tcttcacgtc ctgcgttcca ccgtactgga ggattggcat gaaagtggcg
    18781 aggtgaccgt cgcttcgagc gatcgacacc tcgcttcact ggtcaccttc ctgagacatc
    18841 tggaaccgag gatcgtcgtg gcgcggctga tttgcgaaac accgcgaagc agacgaatag
    18901 cgccgcggtt tccgatcccc aagcaacaaa tcatcgcccg gctttcagcg ttgctggaag
    18961 aggggggata tgttcaagga gatttgcttt gacgacgtcc aagccggaaa gaattattcg
    19021 tcgaatcggt accgtcctga tgataatcgt ggtatgctgt atagggtatt tctctttttc
    19081 caatcggaca ggtggaatat ccctcgggtg ggtgcctttc ggagacaagg gactgcactt
    19141 cctcgtgtat gccgcctacg gattttcctc ctttctggcg ttcttccgag cgcctgagga
    19201 ggaaacggcg aaacgacggg aaggttcggt ggtccatttt tcaacgtggg catcaaaagc
    19261 gatcctgcgg acgcttgtca gcggaacgct gttggggggc atcattgaac tgcttcaacc
    19321 cttggcggga cgacagcggg agatgatgga cctggttgcg aacgtcatgg gcctaatcgt
    19381 gggcttggcg atcatcctgc cggtggtcaa aacgattggg cgtatgcctt ggtttttgat
    19441 cgacaaggag gatgatgagt atgagcttgg cgaaacggat tgaaaaagcg ttgatcgatg
    19501 cagcgcggat tctgcatgaa ccgattcaca aacgactcga atgggcgcgg gaggaggaag
    19561 agcgcctcga cggcaagagc gtcggttcga aggcgagcat cgccgtgctg gaagcgattc
    19621 tggaaaatct gagaatcagc aggaatgaat ggattccgat gtgccaggac accggcatgt
    19681 tcgacgtctt cgtggatgtc ggccgatcct gtccgctttc ccacgccgag atcgaagaag
    19741 ccattctgac cgggtgtgaa gaaggggcga aaaacgggta tttccgtcct tcggtcgtgg
    19801 aagagccgct gttcggaaga aaaaacacga aaaccaatac tcccccgatc atctggtgga
    19861 acctcgttga aggcaggaat gtggaaatca acatccttct gaaagggttt ggaagcgaaa
    19921 acacctgcgc cacccggatg atgaatccca ccgccaccga agagcagatc gtcgccgcgg
    19981 tcgccgaaat cgtcacgatc gccggaggga agccctgtcc cccgatcttc ctcggaatcg
    20041 ggctgggcgg tacgatggag cgcgccgcct acctcagcaa gcgggcgctc cttagagagg
    20101 tgggggtgcc ccacagcgtg gagcggtatg cgaagcttga agagaagatt cttgccgggg
    20161 tccaggcgac cgggatcggt gccggaggcc tcggaggaaa gatcaccgcg ttgaacgtgg
    20221 cggtcgagta tgaacccacc catatcgcag gcttgccgat gggggtttca atcaactgct
    20281 gggcggatcg gatcgcccat atcgtctggg agggctgact atgagaactt tacaccttcc
    20341 gatcagtgaa gaagatctgc aatcgcttcg ggcgtatgat gcagtgagct tttcgggtac
    20401 gttgtatgtc ggtcgcgacc aagtgcatca acggctggcg cgactgcttg aagaggggaa
    20461 gcccctgccg gtggagcttg aggggcaggc gatctactat atggggccgt cacccccgct
    20521 gccggggatg atcatgggct cctgcggtcc gacgacgagc gcccgcatgg atcccttcac
    20581 cccgcagctg cttggaaagg ggttgaaggt tctgatcgga aaaggccctc gatccaaggc
    20641 ggtccatgac gctcttaaaa aagagaaggc ggtctatgtg caggcgttcg gcggctgcgg
    20701 cgccctctac gccaaaacga tcgtcagcgt cgagacggtg gcgttcgaag atctgggacc
    20761 ggaggcgctt ctgcggctgg aagtcaggga ctttcccgcg atcgtcgcca tcgattccca
    20821 cggagggtcg gtattctgat gcaatcgagg ggattcttct ggatttatcc cctcaagtgc
    20881 gatacagttt ctgcatgcag gttgattacc gcccatcctc gtggggtgaa gggtcggcgg
    20941 tgatcaaaat ggttgtatcc gtccgatgaa aggattcaga gtgctgcgaa agataatctt
    21001 tgcaatcacc ccggtgagtt tgatctttct cctcgcgggg tgtccgattc tcatagagcc
    21061 gtctgttttg gatgatgttc ttcccagtgt cgctcagatg atcagcgatg aaggcgggga
    21121 tttgtatacg ttttccgtca gcgatctgcc gaccccttcc acattggaag agcgtttcag
    21181 ctacacctat gggtatatgc tctacaacac cctgcttcag caggacgaga ttgccaatct
    21241 gaacgccccg tatttcgcca aaggggcgtt ggatgcaaac gccggttcag cgatcttcac
    21301 cgccgatgaa atgaatcgga tcctgtttga agttcagctg atgctcctgc aaatcgccca
    21361 gcaggagctt gaagccctca gcaaagccaa ttatgaatac gcccatgcgt tcttggaaga
    21421 gaataagacc cgtgagggaa tcatcactac cgaaagcggt cttcagtata cggtcatcga
    21481 gccgagcgac ggacctcaca tggatccctc cctcagtccc cgagtcaggt tcaccctcac
    21541 cgacggacag ggggaggtcg tcgattccgc cgagagccgg ctcctctccc tcggcaacct
    21601 cactcccggg ttcatcgagg ggctcctctt gatgaacgta ggggggcgtt accgcttctg
    21661 gctcgccccc gatctgacct atcccgacgg cggggaatcc aaccccgccg gtcccaacgc
    21721 tctgctaatt gccgacgtta ctctgcttgg gctcgagtga gaatacgtac atcgccccga
    21781 tggagccgaa gaagttgaga tccaccgaac ccgatccgaa gtagatctcc ggtccggcct
    21841 gagcaaatac tttgatgggg atcttattgg cataccaaga ccaggtgacg gggatgccga
    21901 atgccccgac agcgacgaaa ttaccgctaa aggagccaag atatccggag aatttaccgc
    21961 cgatgctcaa gtaaagatcc attcccaaat cgctgttcac gaatggtttc gggaagttgt
    22021 atccgccgcc cgcccggaac atgaaatacg gtccggtagc gtatcccaac tcgacggatg
    22081 catacgcagg tccgaagtta tactcaccga gcgcaccgac ggtgaacggt attcccgtgg
    22141 aaattcccgc tccgattccc ttcaccggtt cacccgcaaa gagaagggtg gatgcaatca
    22201 gcagcacgag cagcagcaca attcgtttca tgacaaactc ccttggtttt tacgaccttt
    22261 ttgatttttg gatgtattca ttttcatcgg aatcaagctt catgcactaa gagtatggta
    22321 ggaaattgcg ctcgtaaaga gtttgagcgg tattttcgct ataattttat tgaatcgagg
    22381 aggggatgga gctcggacag ggatgtaatc gtgtaggtcg gctcgagggt ggtttcccgg
    22441 ttttcggtgt tgagccagat gcaatcgagt tggacatcaa gcgctccctg aatatcgctg
    22501 gagagggagt cgccgatcat gacgcactcg tgtttcccgg cttcggtcgt ccggagcatg
    22561 tgttcaaaga acccggggtc gggtttcgga actccgatct cggtggagat gaagatgtga
    22621 tcgaagaagc ggtcgaggtt cgaggcttcc agtcgtcccc gttggactcg gctgattcca
    22681 ttggatgcaa ggtggaggcg gtatccctca tccttcaaaa gggtgagaag ggggagggtt
    22741 tcagggtaga gaatcgcttg gcggctgagg tggtcttcgt acacctcgct tgccgattcc
    22801 acatcgaagc gaaggttgct ctgttcttgg aagaggacga accgcttcaa ggagagctca
    22861 tcgatcgtca acagcccctg ttcaaactct ttccagcagg aatggttgca tcgggcatag
    22921 agcaggtgcg aggcgtcatc aatgggaatc cgcaattgcc cggccatccg ggtgaatgcc
    22981 cgcctctcgg cttccctgaa atccatcagt gtcccgtcga ggtcaagaaa aagatggtgg
    23041 tacatgccct aactccttta cttgcaga
//
LOCUS       DAOSLI010000030        22571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2547_length_22571_cov_29.841091, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000030 DAOSLI010000000
VERSION     DAOSLI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 22571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22571
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2547_length_22571_cov_29.841091"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1277
                     /locus_tag="PLF67_05735"
     CDS             291..1277
                     /locus_tag="PLF67_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89549.1"
                     /translation="MKKITIIALAILFLVMPVFAQGVGEKVKTDKLVIWSGAAEDEAA
                     ALVKKFNELHPGVDVSVIRAGSTELVNRLYSEQPRPEGDILLMIAKENMELSYEYLAP
                     YKSVNHDKIDASVRDNAAIPRFYGSSMPLQAIMVNTNLLKPSEYPKGWADLVDPRFKG
                     EIILANPALSGSAYAQLYMMSKQFDDEYLKKLAANTVFTASSTAGPESVARGEYAVTV
                     TGESNIGKYIGQGAPVTYVYPAEGTGARFDATAIIANGPNLENAKLFMDFITSIEGYE
                     IIRTTRNRRVVIKELPGPANLPALGEIKLFPYDDLEAAAIKEELINKFSEFM"
     gene            1339..2451
                     /locus_tag="PLF67_05740"
     CDS             1339..2451
                     /locus_tag="PLF67_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89550.1"
                     /translation="MSLNLTYSKIHKIFDPHSKNPVHALKDVSFSIEPGELFCLLGPS
                     GCGKTTLLRATAGLETITSGHILYGDMDMTSIPPFRRNIGMVFQSFALYPHMNIFENV
                     AYGLRVRKMEEKEIKRRVTEVMELVGLTEELKRNPSPTGLSGGQQQRVAIARALVYDP
                     DILLLDEPLANLDAKLRRYMRAEIRRIQKATNITTIFVTHDQEEAMAIGDRLAVLHKG
                     VVEQIGTSDELYTNPSNAFVANFIGKMNFFNASLVGSHSAKIDATDVTVEVRPERSRL
                     EAGLKKGSEVFVGARPEQLEIVEEASDQTIAATVQIIQHLGQVVRYEVLLDKAISPAT
                     MEIDMPHYLSNVREQDSVFIRFKEGMPFLFNREEQN"
     gene            2451..4157
                     /locus_tag="PLF67_05745"
     CDS             2451..4157
                     /locus_tag="PLF67_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE89551.1"
                     /translation="MSHDPVNAKKMRKDITPYLIFTIPLVFMMIFLMYPMVLTILRAF
                     MPSGNKLQPGAFSLDGFTKFFTSSMYKKALNNSFIVSLSVTGLCILIGVPMGYFVARV
                     KIPGKNMMLSLGILPIIMPSFVGAFTWVILLGRQGVVRHFLNLLLAPLGITIPTIYGM
                     FGMILCMTLTYYPFVFQLAYGAFASANALLEEAGMLMGASRWHIFRTITFPLILPSLG
                     AGALLVFVRAIGNFGIPAIIGGDKYVLPTLIYFRVNGFWDLNGAASIAIVNVAISALV
                     LYLQKRIVSRHEYETISATHSEIRQHDGKAIRIIAFVYCAIILVVSLLPQITIIVMSF
                     FEKWVGLFPEGFTLRHYTRIPAHSHKELFNTFYLSIMATVLAALLGSLVAYITERKKP
                     KGAALLDMSIMMPFILPGTVVSVALLSAFSGNSIIKLTGTYTIIVISYMVRRTPYVYR
                     SVAASLSQLNPSLEEASTIAGANWFYTFRRVSVPLIMPAIISGSILTLTTLLQELSTT
                     ILLYSSKTRTVPIQIYGAVADGKLGEASALSVVLLIVVFVIVYAMNRKQGKSIASAFK
                     MG"
     gene            4161..>4408
                     /locus_tag="PLF67_05750"
     CDS             4161..>4408
                     /locus_tag="PLF67_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89552.1"
                     /translation="MYREHLLTQLLPFWNRAFDELHGGIYTCYTNDGKTLVSRDKYTW
                     SQGRMLWVLSHLLGSPTLAHLLNEEERSRYTERARLLH"
     assembly_gap    4409..4508
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4509..4750
                     /locus_tag="PLF67_05755"
     CDS             <4509..4750
                     /locus_tag="PLF67_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89553.1"
                     /translation="LNEEERSRYTERARLLHIFLDRHAFPAETGNEWIQIRNRSGQPV
                     EGVVALPVKDPFHILRTVMYMTEDEEKTDELPTID"
     gene            4728..6323
                     /locus_tag="PLF67_05760"
     CDS             4728..6323
                     /locus_tag="PLF67_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89554.1"
                     /translation="MNYQQLTEAERITLNTSMFKAYDIRTKSEHVSKELSIRLLEAIA
                     RYYHEILSVDTLVLGRDARLAAPRLMEEALEIFPAFGLDVIVNPLQSSTCQFYFSCMQ
                     HPSAGGIMFTASHNPGEYIGLKLVAPPLQALALGAGPKGGITAIRDFYAAGSNKPSAK
                     TRGRVYIRRYLDQFIDYSRSLAGVHETTLKGVPIMVDFLSGAAGTEVAEALIRSGADL
                     TVRNLIPDGTFPAGEPNPIALESLRPTTELMKRGDWAFGFAFDGDGDRMDLMDRHGVQ
                     VPPSFNLSILIPEIRSYFESVHAQGYWGSAPFNPQMFYDVKANPLSIVLQAKAGMGVH
                     IIRNGHSFIKEALRKNLDDQYVLASEESAHYYMNFPYDLNDLSKGFASTENTLFFALL
                     TARMYSQYPEKYEQAMKLQHSIHRRREWPCHFFDDKHLKPVVNEVERIFADRGLTVFK
                     EMEDGSSLDATLMRKGLPLKIDESSDLEGSWLQVAQRISRSEEGIARWEVASSTKEGL
                     DEAVGLIMAVTDRYVERGEAKYI"
     gene            6414..7607
                     /gene="nagA"
                     /locus_tag="PLF67_05765"
     CDS             6414..7607
                     /gene="nagA"
                     /locus_tag="PLF67_05765"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOE89555.1"
                     /translation="MHLFINATLVLEDSLVHDGYLLEEDGVIKAFGPMPAPSFDGKVI
                     DCKGLYLSPGFVDIHTHGSGGHDYMDGSVEAFVQAAKAHMKFGATTILPTTLSSTDEH
                     LYRSFKDFKAAKATSKGMPHLPGLHLEGPYFCPKEKGAMEERHLRVPDPGHYMPILEA
                     GEGLIRRWSFAPELPGAYEMADVLKKEGIYLSAAHTCANYDQMLKAVDHGVELLTHFY
                     SGMSTITRVGGFRVLGVIESGYLIDGLKLEIIADGLHLPPELLQLIYKCKDADSICAT
                     TDSMRAAGLPEGPSILGPLVGGTDVIVEDGIAKMPDKTVFAGSVCTTDRMVRTLMEHV
                     GLNLPKAVKAASLLPARFVGLDSLTGSIAVGKQADLIIFNEDITIQSVFVSGTNTHIL
                     TDKEE"
     gene            7610..8350
                     /locus_tag="PLF67_05770"
     CDS             7610..8350
                     /locus_tag="PLF67_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOE89556.1"
                     /translation="MSISIFIADSPQELGKKAAKKIVDLLQAAIKEKGEARIILSTGA
                     SQFDTLEALLQSDVDWSKVEMFHLDEYVGLPETHPASFRKYLKERFVSKINLKAVHYV
                     DGEGDIKGKIAKLSALVKEKPVDVGVIGIGENGHIAFNDPPANFDTTDPYMVVELDDR
                     CKQQQIGEGWFATLNDVPKTAITMSPKQIIACKHIVSAVPYAVKAEAVYNTITSKVNP
                     MVPATLLKTHPDWNLFLDQESAAELLAR"
     gene            complement(8352..9194)
                     /locus_tag="PLF67_05775"
     CDS             complement(8352..9194)
                     /locus_tag="PLF67_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE89557.1"
                     /translation="MAKFVPYKNYFYNRLHGMSSLVVASVGTEQSPPGFVHGPEVRTF
                     HTLHFCLKGSGVLRMNTLEHDVEVGDLMLIYPKMKVYPKADRKNPWELIWVGFNGADA
                     RLLTSAIGLSPSSPVVHTNPLSRQRIKQAFDAVYNARGDRPSQIVAMTGALYSLLSLL
                     MELSGTPFPYTSGQEYIDPACDFIALNFNRSITVEDIARHVGVSRSCLYRAFINNLSQ
                     SPQEYLTSYRIKFSCSLLEQSNLPLKEIAYRCGFSSSLYYSKVFKSVMGVPPSEYQMR
                     SNPE"
     gene            complement(9261..10010)
                     /locus_tag="PLF67_05780"
     CDS             complement(9261..10010)
                     /locus_tag="PLF67_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE89558.1"
                     /translation="MNAYLLGYTGSIQRRGVSNTSLAVTAEGRTLLVDLSGSPVQALK
                     EADIDPATIVGVLLTHTHIDHIYALPSLLHQLWLGGRTEPLALISNGPTAAFAKRLID
                     LFDLERKKGFFPLVWHEIEEGDLTIGSLSLRLFPVIHGVPTLGLSVQHHDKHIVYLAD
                     TVYTNMWPSYLQGADILIHEVAGLSEKEETLAGKGHSSARQAAQCALDLKAKRLILVH
                     LPEDPKDDELLLKEAQALFAPSELPRLNVPY"
     gene            complement(10007..11530)
                     /locus_tag="PLF67_05785"
     CDS             complement(10007..11530)
                     /locus_tag="PLF67_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOE89559.1"
                     /translation="MENAFLAGLAQIANLSTLLWMSIGSVVGILLGALPGLTATMGVA
                     LMMPISFQLPTATGIGLLLAVYCGAVSGASIPAILLGIPGNPNAIATVEDGLLMTKRG
                     KAGQALGGAVVASFIGGIGSLLALVLFSPLIARLTLAFGPAEKTTLALVGLVIIASVS
                     GKNIWKGLLMGAFGMGLAFLGTDPFSAQVRVPFASVLSRTPLASGLDLTSTLIGLFGI
                     SQVFYELRKRFEPPQPVQPIEVKDIFPPFKKIASMWKIILSSLGIGTVIGAIPGTGAS
                     IAVFMAHNSAKNITESSNGKLDLVGTGSLEGVFAPEVANNAVTGGALIPALALGIPGD
                     AATAVLIGALMIKGVTPGFHLFATNMSLVYAIFLTMLVANIFMALFQLLGVRLYPKIL
                     SVPEQLLLPQILVLSFIGAYAIAGQATTVGIYNLGIALVMGLIGYALKRDDYPIAPIV
                     LGLILGGMFEENFRRAVKLAGGNYLVFFTKPICLLFIGLSILSVVLPILSRRKKAKQ"
     gene            complement(11547..12281)
                     /locus_tag="PLF67_05790"
     CDS             complement(11547..12281)
                     /locus_tag="PLF67_05790"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOE89560.1"
                     /translation="MSLLKNRKPLPLAFMVLSVLYFIYVVSLGSSRMIGDEIGGDPGG
                     MLLPLVLSIFMFIASTILFITDRADDAYRSGGVQKSQRGLFILTIVVSVLYVALMRHV
                     GFIVTTNTLLVTLTFAYMREGVKREDLALWGGGVVGSLALLIAIYTIGRRVTRYLLLA
                     SRQGVIPKFLGSNGMTFGITLVLVGILFALVYIAGKKLLSRHDAAAPIHSLFQAGFIA
                     MVSTELLYLIFRQLFLVELVRGIISW"
     gene            complement(12297..13280)
                     /locus_tag="PLF67_05795"
     CDS             complement(12297..13280)
                     /locus_tag="PLF67_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOE89561.1"
                     /translation="MKRLRIAITLLLLTTVIGTLAAGGVSETAFPDPEKNITIVVPHA
                     AGGGSDLIYRALAQEMENVGNINFLVTNITGAGSANGTNEVLNHEADGYMVLGAGTHT
                     TAATMQGLTQGYLELDYIAALNWDPFILAVRNDKPWKTFGDLIKDAQSRPGVITLGNA
                     GMGGATGVASVGMNIAFDRSFNVTPFNGGADLIASVLGGHCDVGIFSQSEVVANLTSF
                     RPLVILTEARSTLKDLASVPTFTEAGYPGITLPGGSFRSLMVKKGVPAEAKQWLADTA
                     EKAFNTPTFQKFMKDNGLIPSFFKLDEFAKYDQTTIKDYEAILKDAGLYKM"
     gene            13704..15236
                     /gene="rny"
                     /locus_tag="PLF67_05800"
     CDS             13704..15236
                     /gene="rny"
                     /locus_tag="PLF67_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOE89562.1"
                     /translation="MNNILIILLGCVVGIIFGWLGRWLYAKFKLTSVEQRAIRLNEEA
                     IKEAEAKSKELLLETRDQLLKEQQQQEREARERRTELQRLERRLLQKEENLEQKQTDL
                     DGIKKQLGEWEVNLSQQEEEVAEREGNLIVELERIAGLSADEAKALIMESLYNDAKRD
                     AQLMINKIEQEAQLSADKKAREVVVTSIQRLATEVVNDVTVSSVSLPSDEMKGRIIGR
                     EGRNIRTLETLTGVDVIIDDTPEAVVISCFDPVRKEIARVALERLVQDGRIHPSRIEE
                     VVNKVTKEVGGIIAEEGERVIFDLGIHNVSPEMTRALGRLHFRTSYGQNVLAHAKEVA
                     IISGMIASEIGADRELAVRGGLLHDIGKGIETESDANHSELGADLARRLGEDPRVINA
                     ILSHHNDEEPKTIEAVIVQIADAISAARPGARRETLDNYIKRLESLEKIAESFTGVEK
                     AYAIQAGRELRILVNNEQVSDEGAKEIAKGIAGRIEAELRYPGRIKVTIIRETRVTEY
                     AR"
     gene            15241..16041
                     /locus_tag="PLF67_05805"
     CDS             15241..16041
                     /locus_tag="PLF67_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOE89563.1"
                     /translation="MAEQKPITALLLGDVSGQPGSRALFLGLNTLVKETRADVVIANG
                     ENAANGFGLSVSLMNQFFNLGVHAITSGNHIWQQDELRPFLDSEKRLLRPANYPPQVP
                     GHGSVVLQIREHKVAVLNLQGRQSMSPIDCPFRVGDEHVDRLSKQTKIIIVDFHAENT
                     TEKEALGFYLDGKVSCVVGTHTHVQTADEKILPKGTAYITDLGMCGPTKSVIGSDPVL
                     SIARSISQMPLKSEIADTPPMLQGVIVRIDPITGKALSIERFTRCFSV"
     gene            16038..16817
                     /locus_tag="PLF67_05810"
     CDS             16038..16817
                     /locus_tag="PLF67_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOE89564.1"
                     /translation="MKNVPLLQALLKTHPAYTKDELHAFIVCRNVYVDGELVSDPKQG
                     VDEGAIFEIHIRKYVSRGGYKLEHALSSFKIDVSGEVFLDAGSSTGGFTDCLLQHGAK
                     LVHAVDVGYNLIDWKLRSDERVIVHERQNIMTLAEPSPPPTAAVCDLSFRSIRKAAAH
                     ILDLVGNTYLIALIKPQFEMENPPPSFDGVIEDPGLIVQVVTQLFLSLAEEGVGVFDL
                     VASPIRGAKGNIEFLALLKKGQGLSLKLLTHQVEAQLELLQ"
     gene            complement(16777..17307)
                     /locus_tag="PLF67_05815"
     CDS             complement(16777..17307)
                     /locus_tag="PLF67_05815"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOE89565.1"
                     /translation="MTNHPKALLRALIILLLLILYTTIIGIVSFPTVKRAFHESGVLQ
                     LIRSPKEQTQASLSKRMVSVVFAPLGTSFAVYTVEQPRLGSSASHDTFEALLSGVPLT
                     ALKDGAITYIASGTKLLGLTRSSNIIYLNLSKEFLASSNMELAYRQLKETASLLGAKD
                     IVVLIEGAQVELQPDE"
     gene            17382..19115
                     /gene="ptsP"
                     /locus_tag="PLF67_05820"
     CDS             17382..19115
                     /gene="ptsP"
                     /locus_tag="PLF67_05820"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOE89566.1"
                     /translation="MRIFTGLAIGEGIARGKAKIYRHTPIVVERKRIAKSQVKREHGL
                     LDQAFQRARTALERRKGNHLEGEALEILETHLLLLQDPTYITSIKEKIEDKLINAAWA
                     TEEVTEGIVKTLEASDDELFRQRTIDINDIGDSIVEHLCGKSAHPSLVLDEPVILLAD
                     TLLPSELFSLDRSNILGLSLDGGGLASHVAILARGLNIPTVIETQLASHVAEDGDDVI
                     IDGIRGEVIIRADHQAVQNVTERYNAYLEHEAELLKGASLEARTKDGKRILIKSNIQT
                     LGELPFAEAEGSDGIGLFRTEFFFMERSALPSEEEQFRIYKEVLERVAPRSVTIRTLD
                     LGGDKVVSGLNLVEENPVLGFRAVRFCLEHQDIFLTQMRAILRASPFGKADIMFPMVS
                     GIGELKETLRLFELAKRQLKEEGVSFDPDLQVGTMIEVPSAALITDLIVRHVDFISIG
                     TNDLIQYTIAVDRNNQKIAHLYQPLHPGVLRLLKGVVDQAHGMGKRVAVCGEMASDPH
                     HAVVLVGLGVDELSMGPHALLKLRKIVRSLDLAAAQSLVEHLLTMDSSVRTEQAVREW
                     MHERFDDLTIR"
     gene            19084..20619
                     /gene="der"
                     /locus_tag="PLF67_05825"
     CDS             19084..20619
                     /gene="der"
                     /locus_tag="PLF67_05825"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOE89567.1"
                     /translation="MNDSMTLLSDDPVVVSIIGRPNVGKSTLFNRLIGRRRAITDPTP
                     GVTRDLLPERWYLAGNPVTLVDSGGVKMDGQTLDEMVSAKSLSLLKRSDAIIFMMDCT
                     EVTAEDLHLLEALRPYGDRMVLTVNKVDDPKREDLVWEFYGYGYERVVGISAAHGLGI
                     DDLEELLLGMLDFEEIEQTEEEETTVKLAIMGKPNTGKSTLANLLVGRQISLVSEIAG
                     TTRDVVSGSFVHKGTTFNVLDTAGIRRKSRVDEDIEYYSVNRAIRTIEEADVVFLVVD
                     SAEGLADQDKKIAQLIVRRGKGVVIVLNKIDLLSGVPNELEAIRDRIRFLFPVLNFAP
                     VVMISALEEKGIEPLLDTAWSLYRQLHRRIETSHLNEALRRWGEEYPPPRGREGHFKV
                     YYGTQVSTNPVNFLLFVNRMKEFPASYLTYLTNKIRSELGFPQIPVEIDLKERRRSPS
                     LNLKGPKPIQDREEKREVVTPRRTGGKAVARKKGVKPGNKAAVEKARRRAIKEQQQRK
                     GGK"
     gene            20619..21302
                     /locus_tag="PLF67_05830"
     CDS             20619..21302
                     /locus_tag="PLF67_05830"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE89568.1"
                     /translation="MGFIAEAGITTLALDIDGTLYSRFQLNTRMIRSLFPNVRLAIAF
                     NRARKEVRRIQDEYPPDDETREGFLDRQAALVAERYKKDVGMVKELIGTQFYQAWERT
                     FTTITPFHHLRETLEQVKSEGVRIILFSDFPIAEKPTTLGIADLVDDAFTSEESGYLK
                     PSVKAFEYLIGKSGAERSKILYIGDSYDKDCIGATDAGLVSWYLGKKKAGPKASRSFA
                     SWSEIKAVI"
     gene            21315..21821
                     /locus_tag="PLF67_05835"
     CDS             21315..21821
                     /locus_tag="PLF67_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89569.1"
                     /translation="MGLEIILSLLLFVITLIIMLLLRARDKRSHSLEVMKNRMGQFIK
                     EIDATRSLFREEMQSVEERLNWVIDEARRGSEALSSRLDEIDRRRSELIELESILARY
                     RNTLLELGETTLAIDRKIEEQKTWQKQIDDIHAQIDEFDFRLQGFASSFERALKSAYK
                     AMEEPLLT"
     gene            21920..22465
                     /locus_tag="PLF67_05840"
     CDS             21920..22465
                     /locus_tag="PLF67_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOE89570.1"
                     /translation="MSKKEEAKKKETVKKEDTEELVEDLQSIEDELAEARREIAALKE
                     ENLRDRADLENWRKRMLRDKEDSIRFANERLIIDLLGPLDDFERAIEAAEEAQDFSKV
                     HDGVVLVSEQIYSTLQKNWGLERIEAVGKEFDPNEHEAYQVVEDENLEHETVLEEYAT
                     GYKLHGRVLRPSKVKVGRPAI"
BASE COUNT         5502 a         5770 c         6314 g         4885 t
ORIGIN      
        1 gctctttcta ttgggcttgc attgtataac gtgaaaaaga aggaggcgta gggattccaa
       61 gagagaagct gcaagagcac gcaacagtga tggtaccggt tttgatgaat tcaaggccgg
      121 tattttttat tggaacaaat ttgcttatcc ttgtctaaat ttgcttaata gatagagtgt
      181 attagataaa acaacaaaaa gaaaactttt acttgcgtta ttgttaaagg tatccgatac
      241 taaagaaggt ggcatcgaat ggtgtcgcaa gcacacattg gaggtactct atgaaaaaaa
      301 tcactatcat tgcgctggca atcctgtttt tagtgatgcc tgtgttcgcc cagggcgtcg
      361 gcgaaaaagt aaaaaccgac aaacttgtca tttggagcgg agctgccgaa gatgaagcgg
      421 ccgcattggt caaaaaattc aatgaactgc atcccggcgt tgatgtaagc gtcattcggg
      481 cgggatcgac cgagctggta aaccgtctgt attccgaaca gccgcgcccg gaaggggata
      541 tccttttgat gatcgcaaag gagaacatgg agctttccta cgaatacctt gcaccgtata
      601 aaagcgtcaa ccacgataag attgatgcat cggttcgcga caatgccgca atcccccgct
      661 tctatggttc ttccatgccc cttcaagcga ttatggtcaa caccaacctg ctgaagccga
      721 gcgagtaccc gaaggggtgg gccgatttgg tcgatccacg cttcaaaggg gagatcatcc
      781 ttgcaaaccc ggcgctttcc ggctccgcgt atgcccagct gtacatgatg tcgaagcagt
      841 tcgacgacga atacttgaaa aagctcgccg ccaacacggt gttcacggca agctccaccg
      901 ccggaccgga atcggtcgcc cggggtgagt acgcggttac cgtaaccggt gaatcaaaca
      961 tcggcaaata catcggccaa ggcgctccgg taacgtatgt gtatccggcg gaaggaaccg
     1021 gtgcccgctt tgatgcaacg gcgatcatcg ccaacggacc gaaccttgag aatgcaaagc
     1081 tgttcatgga cttcatcacg agcatcgaag ggtatgagat cattcgaacc acgagaaacc
     1141 gccgcgtagt catcaaagaa ctgcccggcc cggctaatct tccggctctc ggggagatca
     1201 aactcttccc gtacgatgat cttgaagcag ccgcaatcaa ggaagaactg atcaacaagt
     1261 tctccgagtt catgtaatat caccgctgcc cggggcaccc gggcagcccc atcttcttaa
     1321 gaggagtgta cgccaccgat gagtctcaat ctgacctatt cgaagatcca taaaatattt
     1381 gatccccatt caaaaaatcc cgttcatgcg ttgaaggatg tttcattctc gatcgagcca
     1441 ggggagttgt tctgtcttct cggaccctcg gggtgcggca agacgacgtt gctgcgggca
     1501 accgcgggtt tggagacgat cacaagcggt catattctct atggtgatat ggacatgacc
     1561 tcgatcccgc cgtttcggcg gaatatcggg atggtattcc aatcgtttgc tctttatcca
     1621 catatgaaca tctttgaaaa cgtcgcctac gggcttcgtg ttcgaaagat ggaggagaaa
     1681 gagataaaaa ggcgcgtcac cgaagtgatg gagcttgtcg gtctgaccga ggagttgaag
     1741 agaaaccctt caccgaccgg cctttccggc gggcagcagc aacgcgtggc gatcgcccgg
     1801 gcattggtat atgatccgga tattcttctg cttgatgaac cgctggccaa cttggacgcg
     1861 aagcttcggc gctatatgcg ggcggagatt cgacggattc aaaaagcgac gaacatcaca
     1921 accattttcg tcacccacga tcaggaagag gcgatggcga tcggtgaccg cctcgcggtg
     1981 ctgcacaaag gggtcgtcga acagattggc accagcgacg agctgtatac gaatccttca
     2041 aacgcttttg ttgccaactt catcggcaag atgaacttct tcaacgcttc tctggtaggc
     2101 tcccactcgg cgaagatcga tgccacggac gtgacggtgg aggtgaggcc cgaacgatcc
     2161 cggttggaag cgggcttgaa aaaggggagc gaggtctttg tgggggctcg ccccgagcag
     2221 cttgaaatcg tcgaagaggc gagtgatcaa acgatcgccg cgacggtcca gatcattcag
     2281 cacctcggac aggtcgttcg atacgaagtg ttgttggaca aggcgatcag ccccgcgacg
     2341 atggaaatcg atatgcccca ttacttgagc aatgtccggg aacaggattc ggtcttcatc
     2401 cgcttcaagg agggaatgcc tttcctcttc aaccgggagg agcagaactg atgagtcacg
     2461 accctgtcaa tgcaaagaag atgcgcaagg atataacgcc gtatctgata ttcaccattc
     2521 ccttggtgtt catgatgatt ttccttatgt atccgatggt tttgacgatt ctgcgggcgt
     2581 tcatgccctc gggaaacaaa ctgcagccgg gtgctttttc gctcgacggg tttacgaagt
     2641 tcttcaccag ttcaatgtac aaaaaagcgt tgaacaactc gttcatcgtc agcctctcgg
     2701 taacggggct ctgcattctc atcggagttc cgatggggta cttcgtggct cgggtaaaga
     2761 ttcccggtaa gaacatgatg ctcagtctgg gaatccttcc catcatcatg ccatccttcg
     2821 tcggagcgtt cacgtgggtc atcctgctcg ggcgccaggg cgtggttcgc cacttcctga
     2881 atcttcttct tgccccgctg ggaatcacga tcccgacgat ctacggaatg ttcgggatga
     2941 tattgtgcat gacgctgacc tactatccct ttgttttcca gctcgcctac ggggcgttcg
     3001 cttcggcgaa tgccctgctt gaggaggcgg ggatgctgat gggggcatcc cgctggcata
     3061 tctttagaac gatcaccttc cccctgattc ttccgagtct tggagctgga gctctgctcg
     3121 tgtttgtccg ggcgatcggg aacttcggaa ttcccgcgat catcggtggc gacaagtatg
     3181 tgcttccgac cctgatctac ttcagagtca acgggttctg ggatctcaac ggggcggcct
     3241 cgatagcgat cgtgaacgtt gcgatatccg ctctcgttct ctatctccag aaacgaattg
     3301 tcagcaggca tgagtatgag acgatttctg ctacccactc ggagatcaga cagcacgacg
     3361 gcaaagcgat tcggatcatt gcgttcgtgt actgtgcgat catcctcgtc gtctccctgc
     3421 tgccccagat cacgatcatc gtgatgtcct tctttgaaaa gtgggtaggt ctcttccccg
     3481 aagggtttac cctccgccac tatacccgaa ttccggcaca ttcgcacaaa gagctgttca
     3541 acaccttcta tctttccatc atggcgacgg ttttggctgc attgctcggc tcgctcgtcg
     3601 cctatatcac cgagcggaag aaaccgaaag gggcagcctt gctggatatg tcgatcatga
     3661 tgccgttcat cctccccggg accgtggttt cggtcgctct tttgtccgcg ttcagcggga
     3721 acagcatcat caaactgacg ggcacgtata cgatcatcgt catctcatac atggtcaggc
     3781 ggaccccgta tgtgtatcga agcgtggcgg cgagcctctc gcagctcaac ccttcgcttg
     3841 aagaggcatc gacgatcgcc ggggcgaatt ggttttatac attccgtcgg gtcagtgtgc
     3901 cgctgatcat gccggcgatc atcagcggat cgattttgac gttgacgacc ttgcttcagg
     3961 agttgagtac gacgattctt ctgtacagct cgaaaacgag aaccgtgccg attcagatct
     4021 atggcgctgt tgcggatgga aagctcggcg aggcgagcgc gttgtcggtg gttcttctca
     4081 ttgtcgtctt cgtgatcgtg tacgcgatga accggaaaca agggaagtcg atcgcttcgg
     4141 cattcaagat ggggtagatg atgtatcggg aacatttgct gactcagctt cttccctttt
     4201 ggaatcgggc atttgatgag ctccacggtg gaatctacac ctgctatacc aatgacggca
     4261 agacgcttgt ttcccgagac aagtatacgt ggagccaagg ccgaatgctt tgggttctct
     4321 cgcatctcct tggctcaccg acccttgcac acctattgaa tgaggaggaa cgaagccggt
     4381 atacggagcg ggcgcggctc ttgcacatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnta ttgaatgagg aggaacgaag ccggtatacg gagcgggcgc ggctcttgca
     4561 catatttctt gaccggcatg cattccccgc cgagacggga aatgaatgga tccagattcg
     4621 aaaccgatcg ggtcaaccgg ttgaaggggt tgtcgccctt ccggtaaaag atccgtttca
     4681 tattttgaga acagtaatgt atatgaccga agatgaggag aagaccgatg aactaccaac
     4741 aattgactga agcagagcga attacgctga atacgagtat gttcaaggca tacgacatcc
     4801 ggaccaaaag cgaacatgta tccaaagaat tatccattcg tcttcttgag gcgattgctc
     4861 gatactatca cgagattctt tcggttgata cgctcgtcct tggccgtgat gcccgcttgg
     4921 cggccccccg actgatggag gaggcactgg agatttttcc ggcgttcggg ttggatgtga
     4981 tcgtgaaccc cctccaaagc tcgacgtgtc aattttactt cagttgcatg cagcatccat
     5041 ccgccggagg aatcatgttc accgcgagtc acaaccccgg cgaatacatc ggattgaaac
     5101 tggtcgcccc gccgttgcaa gcgttggcgc tcggagccgg gccgaaagga ggcatcacag
     5161 caatccgtga tttctatgct gccggatcca ataagccctc ggcaaaaaca cgagggcggg
     5221 tgtatatcag gcggtacctc gatcaattca tcgattactc ccgctccctt gccggtgtgc
     5281 atgaaaccac cttgaaaggg gtgccgatca tggtcgattt cctcagcggg gctgccggaa
     5341 cggaagttgc cgaggcgctg atccgaagcg gggcggatct taccgttcgg aatctcattc
     5401 ccgatggcac cttccccgcc ggggaaccca acccgatagc cttggagtcg ctgagaccga
     5461 cgaccgagct gatgaagcgc ggagattggg cattcgggtt tgcgttcgac ggcgatggag
     5521 atcggatgga tctgatggac cggcacggag tgcaggttcc tccttcgttc aatctgtcca
     5581 tcctcatccc cgaaatccgc tcctatttcg aatcggttca cgctcaaggg tactggggtt
     5641 ccgctccctt taacccgcag atgttttacg atgtgaaagc aaatcctctt tccatcgtcc
     5701 ttcaggcaaa agcgggaatg ggtgttcaca tcattcgaaa cgggcactct ttcatcaaag
     5761 aagcgcttag gaagaacctt gatgatcaat acgtgctcgc cagcgaagaa tccgcccatt
     5821 actacatgaa ctttccctat gacctcaacg atctttccaa agggttcgcc tccacggaaa
     5881 acacgctgtt ctttgctctc ctgacagcta ggatgtacag ccaatatccc gaaaaatatg
     5941 aacaggcgat gaagctgcag cactcgattc accgtcggag agaatggcca tgtcatttct
     6001 ttgatgacaa gcaccttaaa ccggtagtca acgaggttga acgcatcttt gccgaccggg
     6061 ggctgacggt cttcaaggag atggaagacg gatcttccct ggatgcgacc ttgatgcgca
     6121 aaggccttcc cttgaaaatc gatgagtcct ccgacttgga gggatcttgg ttgcaagtcg
     6181 cccaacgaat cagccgaagc gaagaaggga ttgcccgctg ggaggtcgct tcatccacga
     6241 aggaagggct tgatgaggcg gtcgggttaa tcatggctgt tacggatcgg tatgtggaac
     6301 gcggagaagc caaatacata taaatgcaac ataatgatat gctcctgcaa caaagtaatg
     6361 tggttggcgg aatagggaca tggtaccgtg tacacaagag tgaggagggg taagtgcacc
     6421 tgtttatcaa tgcaaccttg gtccttgagg actcgcttgt tcatgatggg tatcttcttg
     6481 aggaagatgg tgttatcaag gcgtttggac cgatgccggc accttcgttc gacgggaagg
     6541 tgatcgactg caaaggtctc tatctgagcc ccggtttcgt tgatatccat acccacggtt
     6601 ccggcggaca cgattatatg gacggaagcg tcgaagcgtt cgttcaagcc gccaaggcgc
     6661 atatgaaatt cggtgcgacc acaattttgc cgaccaccct ttcttccacc gatgaacacc
     6721 tctatcgctc attcaaggat tttaaagctg caaaagccac ctcgaagggg atgccgcacc
     6781 tgcctggtct ccatctggag ggaccctatt tctgccccaa ggaaaaagga gcgatggagg
     6841 agcgccatct tcgagtgccg gaccctggac actatatgcc gattctggaa gccggcgaag
     6901 ggttgatccg gcgctggtcg ttcgcccccg aactgccggg cgcctatgag atggcggatg
     6961 tgctcaagaa agaagggatt tacctttccg ccgcccatac ctgcgccaat tacgatcaga
     7021 tgctgaaggc ggtcgatcat ggggtcgagc tgttgaccca cttctattcg gggatgtcaa
     7081 cgatcacccg ggtcggcggg ttcagagtac tgggagtgat cgaaagcggg tatctcatcg
     7141 atggtctgaa gctggagatc atcgccgacg gacttcacct accaccggag ctgcttcagc
     7201 tgatctacaa gtgcaaggat gccgacagca tctgtgccac caccgacagc atgcgcgccg
     7261 ccgggttgcc ggaagggccg tcgatcctcg gtccgcttgt aggcggaacc gacgtgatcg
     7321 tcgaggacgg aatcgccaag atgcccgaca agaccgtatt cgccggcagt gtgtgcacca
     7381 cggaccggat ggtccggaca ctcatggaac acgtcggttt gaatctgccg aaagcggtga
     7441 aagctgcaag cctgctaccg gcgcggttcg taggcctcga ttccctgacg ggatcgatcg
     7501 ccgtgggaaa gcaggccgat ttaattatct tcaatgaaga tattacaata caaagtgtgt
     7561 ttgtgagtgg aacgaacact cacatattga cagataagga ggagtaacca tgagtatttc
     7621 gatttttatt gccgatagcc cgcaagagct gggaaaaaaa gcagccaaga agattgtcga
     7681 tctcttgcag gcggcgatca aagagaaagg tgaagccaga atcattcttt ccaccggggc
     7741 cagccagttc gatacgctgg aagcgttgct tcaatccgat gtcgattggt ccaaggtgga
     7801 gatgttccac ctcgatgaat acgtgggcct gccggaaacc caccctgcaa gcttccgcaa
     7861 gtatctgaaa gaacgctttg tttccaagat caatctgaaa gccgtccact atgtcgatgg
     7921 ggaaggtgat atcaaaggaa agattgcgaa gctttccgct cttgtcaagg aaaaaccggt
     7981 cgatgtcggg gtaatcggaa tcggtgaaaa cgggcatatc gccttcaacg atcctccggc
     8041 gaatttcgat accaccgatc cgtatatggt cgtcgaattg gacgatcgct gcaagcagca
     8101 gcaaataggc gaagggtggt tcgcaacgct caatgatgtt cccaagactg cgatcacgat
     8161 gagtccaaag cagattattg cgtgcaagca catcgtttcg gcggttccgt acgcggtaaa
     8221 agcggaagcg gtctacaaca cgattacatc gaaagtcaat ccgatggttc cggctacgct
     8281 tctgaagacg caccccgact ggaacctgtt cctcgatcag gagtcagcgg cggaactgct
     8341 tgcccgttag atcattcggg attggagcgc atctgatact cggagggtgg cacgcccatc
     8401 acggatttga acaccttcga gtagtacagc gaactggaaa acccacaacg atatgcaatc
     8461 tccttaagcg ggagattgct ttgttccaga aggctgcatg aaaattttat ccggtaggag
     8521 gtcaggtact cctgaggtga ttgggagagg ttgttgatga atgcccggta aagacaggaa
     8581 cggctgactc cgacatgccg ggcgatatct tcaacggtta ttgatcggtt gaagttgagc
     8641 gcaatgaaat cgcacgccgg atcgatatat tcctgaccgg aggtgtaggg aaaaggcgtg
     8701 ccgctgagtt ccatcagaag cgagagcagg gagtacaatg caccggtcat cgccacgatc
     8761 tgactggggc gatcgcctcg ggcgttatag actgcatcga aggcctgttt gattcgctgg
     8821 cggctaagcg gattcgtgtg aacgaccgga gaggagggcg aaagtccgat cgcgctggtg
     8881 agcagccggg catccgctcc gttgaagccc acccatataa gctcccaggg attttttcgg
     8941 tccgctttgg gatatacctt cattttagga tagatcagca tgaggtcgcc gacttccaca
     9001 tcatgctcca aggtgttcat tctcagtact cccgatccct tgaggcagaa gtggagggta
     9061 tggaaggtgc gtacttccgg gccatggaca aaccccggcg gactctgctc ggttcccacc
     9121 gaggcaacca cgagcgaaga catgccgtgc aggcgattat agaagtagtt tttgtatggg
     9181 acgaatttgg ccatacgtca cgataccaag aagaagatgc tcacgcaatg gagagaagct
     9241 catcctgtat gccggtgaaa tcaatacggt acgttaagtc ggggcagctc gcttggcgca
     9301 aagagcgctt gagcttcttt caagaggagc tcatcatctt tgggatcttc aggaaggtga
     9361 acgagaatca agcgcttcgc cttcaaatcg agggcgcatt gtgcggcctg acgggcggaa
     9421 gagtgccctt tcccggcaag cgtctcctcc ttctcgctca gacctgccac ttcgtggatc
     9481 aggatatccg ccccttgcag atatgaaggc cacatattgg tgtatacggt atcggcaagg
     9541 tacacgatgt gtttgtcgtg atgctgaacg gaaagaccca atgtcggtac tccgtggatg
     9601 acgggaaaga gtctcaagga gagcgatccg attgtaagat ctccctcttc gatctcatgc
     9661 cagacgagcg gaaagaaccc ttttttccgt tccagatcga acagatcgat gaggcgtttg
     9721 gcaaacgccg ccgtcggacc attggagatg agagccagcg gctcggtccg gccgccgagc
     9781 cagagctggt gaagcagcga gggaagcgcg tagatatggt cgatatgggt gtgggtaagc
     9841 aggaccccta cgattgttgc ggggtctata tcggcttctt tgagcgcttg aacgggtgat
     9901 cccgagagat cgacgaggag agtcctcccc tccgcagtaa ccgccaggga ggtgttggac
     9961 accccccggc gttgtatcga gcctgtgtat cctaagagat acgcgttcat tgtttggcct
    10021 ttttacgacg ggaaagaatc ggcagaacga cggagaggat cgacagaccg ataaacagca
    10081 ggcagatcgg cttggtgaag aacaccagat aattgccgcc tgcgagcttg acggcccggc
    10141 ggaagttctc ctcgaacatt cctccgagga tgagtccgag aacgatcgga gcgatcggat
    10201 agtcgtcccg tttcagagca tatccgatca gtcccatcac gagcgcgatc ccaaggttat
    10261 agattccgac ggtcgtcgcc tggccggcga tcgcatatgc accgatgaag gaaaggacaa
    10321 gaatctgcgg caagaggagc tgctcgggaa cgcttaaaat cttgggataa agcctgacac
    10381 ccagcagctg gaagagggcc atgaagatat tcgccacgag catcgtgagg aagatcgcat
    10441 agaccaaaga catattggtg gcaaaaaggt gaaagcccgg cgtcacgccc ttgatcatca
    10501 aagcgccgat gagtacggcg gtcgccgcat cgccggggat tcccagcgca agggcgggaa
    10561 tgagggcacc gcctgtgacg gcgttgtttg cgacttcggg agcaaacacc ccttccaggg
    10621 agcccgttcc aaccaaatcc agtttaccgt tggagctttc agtgatgttt tttgccgagt
    10681 tatgcgccat gaagactgcg attgaagctc ccgttcccgg gatcgccccg atgaccgttc
    10741 cgattccgag actgcttaaa atgatcttcc acatcgaagc gatttttttg aacggaggga
    10801 agatgtcctt gacttcaatc ggttgtacgg gctgtggggg ttcaaaacgt ttgcgcaatt
    10861 catagaacac ctgagagatg ccaaagagtc cgatcagggt ggaagtcaga tccaaaccgc
    10921 tggccaaggg ggttcgggaa agaacgcttg caaacggtac cctgacctga gcgctgaacg
    10981 ggtcggtacc taagaatgcc aatcccattc cgaacgctcc catgagcaac cccttccaaa
    11041 tatttttacc cgagacggat gcaatgatga ccaacccgac gagggcgaga gtggtttttt
    11101 ccgccggacc gaaggcaagg gtgagccgtg caatgagagg gctgaagagc acgagagcca
    11161 gcagacttcc gattccaccg atgaaggaag cgaccacagc cccgccgagc gcctgacccg
    11221 cctttccccg ttttgtcatc aagagtccgt cttcaaccgt cgcgatagca ttcgggtttc
    11281 cggggattcc gagcagaatc gccgggatgg aagcaccgga taccgctccg caatacacgg
    11341 cgagcagcaa cccgattccc gttgcggtgg gcagctggaa gctgatcggc atcatcaagg
    11401 cgactcccat cgtggcggtc agtcccggca aggcgccgag aagaataccg actaccgacc
    11461 ctatcgacat ccacaagagc gtggagagat ttgcaatttg agccaagcct gcaagaaaag
    11521 cattttccat agtgtatcct ccgctcctac catgagatta tgccgcgaac caattctaca
    11581 aggaacagtt ggcggaaaat gagatataac agctccgtgc tgaccatggc aatgaatccc
    11641 gcttgaaaga gggagtgaat cggagcagcc gcgtcatggc gagacaagag ttttttcccc
    11701 gcaatgtata cgagggcaaa cagaatgccc accaaaacaa gggtgatacc gaacgtcatg
    11761 ccattggaac cgagaaactt tggaatgacc ccttgtcggg aggcgaggag aagatagcga
    11821 gttacccgtc ggccaatcgt atagattgcg atcaggaggg cgagagagcc gacgacgcct
    11881 ccgccccaca atgcaaggtc ttcccgcttt acgccttcac gcatatacgc gaaagtcagc
    11941 gtaacgagca aggtgtttgt cgtaacgata aaccctacgt ggcgcataag cgccacgtag
    12001 agaaccgaaa caacaatcgt caggataaag aggccgcgct gagacttctg aacccctccg
    12061 ctccgatatg catcatcagc ccgatccgtg atgaacagga tcgtggaagc gatgaacatg
    12121 aagattgaca aaaccagcgg caggagcatc ccacccgggt ctccgccaat ttcatcaccg
    12181 atcatcctgg atgagccgag gctcaccaca tagatgaaat acaataccga gaggaccata
    12241 aacgctaacg gcaggggttt tcgatttttc agcaatgaca tcttcttacc accttattac
    12301 atcttgtaga gaccggcatc cttcagaata gcttcgtagt ctttaatggt agtctgatcg
    12361 tacttggcaa attcgtcaag tttaaagaag gaggggatca ggccgttatc cttcatgaac
    12421 ttttggaagg tgggggtgtt gaacgccttt tccgccgtat cggcaagcca ctgcttcgct
    12481 tcggcgggga ctcccttttt gaccatcaac gagcggaacg atccgccggg aagggtgatt
    12541 cccgggtagc ctgcttcggt gaacgtggga accgaggcca gatccttgag ggtggagcgt
    12601 gcttccgtca agattaccaa aggtcggaag gaggtcaagt ttgcgacaac ctccgactgg
    12661 ctgaagatac ccacatcaca gtgccctccg aggacgctgg cgatcaggtc cgcacctccg
    12721 ttaaacggag ttacgttgaa gcttctgtcg aacgcgatgt tcattccgac tgatgcgact
    12781 ccggtggcac cgcccattcc ggcgtttcca agagtgatta ctcctgggcg gctttgggca
    12841 tctttgatca gatcgccgaa ggttttccag ggcttgtcgt ttcgtaccgc caaaatgaac
    12901 ggatcccagt tgagggccgc gatgtagtcc agctcaaggt atccctgggt caagccctgc
    12961 atcgtggctg cagtggtatg cgttcccgct cccaagacca tatatccgtc ggcttcatgg
    13021 ttcaggacct catttgtgcc gttggcgctg cccgcaccgg ttatgttggt aacaaggaaa
    13081 ttgatgttcc cgacattctc catctcctgg gcaagagctc tgtagatcaa gtccgaacca
    13141 ccgccagcag cgtgcggtac gacgatggtg atgttttttt ccggatcggg gaaagccgtt
    13201 tcgctgactc cgccggcggc cagtgttcct atcacggtgg tgagaagcaa caacgtaata
    13261 gcaattcgta gacgtttcat agttcgttcc tccatacatt tccctaacaa acagttaggt
    13321 gttattccaa gaaaagcaac tttaaatttg ccttttttat tattataacc tataagacaa
    13381 ccaaaagcaa ataaaaaaaa catgaaaaag cataaaatgt tgcataaaac gcaaaaacat
    13441 acacataata taggcttaaa gagataattt cagctttgat gaggaaaagt gcatgctttt
    13501 ctgcagtcgg agggaacgtt cccggttctt cgattgcggg gcgatacttg agaagaagaa
    13561 tcgatgtata gaatttgttg ggttataagt atatgcaatt aaataaataa cactgcaata
    13621 taggttgatt ccaccttcct gccaagcctt gacaattccg acaggcgttc gtatgattga
    13681 gctcatacga tagggggtag tgtatgaata atatactcat tatcctcctt ggttgtgttg
    13741 ttggtatcat ctttggttgg ttgggccgat ggctgtatgc gaaatttaag cttacttccg
    13801 ttgagcagcg agcgattcgc cttaacgagg aagcaataaa ggaagcggaa gcaaaaagca
    13861 aggagctttt gcttgaaact cgggatcagt tgctgaaaga gcagcaacag caggaacgag
    13921 aggcgcgtga gagacgaact gaactgcaac gattagagcg aagactcctt cagaaagagg
    13981 agaatctcga gcagaagcag actgatctgg atggtattaa aaaacaattg ggagagtggg
    14041 aagtaaatct ttcccagcag gaagaagaag ttgccgaaag ggaaggcaac ttgattgtgg
    14101 aacttgaacg catcgctgga ttgtcggcgg atgaggcgaa agcccttatc atggagagct
    14161 tgtacaacga tgcgaaaaga gacgcccagc tcatgatcaa caagattgag caagaggcac
    14221 agctctccgc cgacaaaaaa gcccgagaag tcgttgttac ctcgatccag aggttggcga
    14281 ccgaggtcgt gaatgatgtc accgtcagtt cggtgagcct tccatccgat gagatgaaag
    14341 gaaggatcat cggacgcgag ggaaggaata tccgaaccct tgagaccctt accggggtcg
    14401 atgtgatcat tgacgacacc cctgaagcag tggtcatctc ctgcttcgat ccggtgcgca
    14461 aggagatcgc gcgtgttgcg ctcgagcgcc ttgttcagga tggacgaatc catccctcac
    14521 ggattgaaga ggtggtcaac aaagtcacca aggaagttgg tggcatcatt gccgaagagg
    14581 gcgaacgggt catctttgat ctggggatac ataatgtatc acccgagatg acgcgggctt
    14641 tgggccgtct tcatttccgc accagctatg gacagaacgt ccttgcccac gccaaggagg
    14701 tcgcaatcat ctccggcatg atcgccagtg agatcggtgc tgaccgcgag cttgccgtcc
    14761 gtggtggatt gctccacgac atcggcaagg gaattgaaac ggagagtgat gcaaaccact
    14821 ccgaactcgg agcggatctt gcccggcggc ttggtgagga tccacgagta atcaatgcga
    14881 ttctctccca tcacaacgat gaggagccga aaacgatcga ggcggtgatc gttcagatag
    14941 ccgatgcgat cagcgcagcg cgccccggcg cccgccggga gacgctggac aactacatca
    15001 agcgccttga atcacttgag aagatcgctg aatcgttcac gggagtcgaa aaagcgtacg
    15061 caatccaagc gggccgcgaa ttgagaatcc ttgtgaacaa cgagcaggtc tcggatgaag
    15121 gggccaaaga gatcgcaaaa ggaatcgccg gacgaatcga agcggagctt cgctaccccg
    15181 gtcgaatcaa agtcacgatc atccgcgaaa cccgggtgac cgagtacgcc aggtaagatc
    15241 gtggctgagc aaaaaccgat aacggccttg cttctcgggg atgtctccgg acagcccggg
    15301 agcagggctc tctttttggg cttgaacacc cttgtcaaag agacgagggc ggatgtggtg
    15361 atcgccaacg gtgagaacgc cgccaacggg ttcggtcttt ccgtatcgtt gatgaaccag
    15421 ttcttcaatc tcggcgtcca tgcgatcaca agcggaaacc atatttggca gcaggatgag
    15481 ctgcgaccgt tcctcgactc ggagaagcgg ctgctcaggc cggcgaacta tccgccccaa
    15541 gtccccggcc acgggagcgt ggttcttcag attagggagc ataaagtcgc ggtcttgaac
    15601 cttcaaggtc gccagtcgat gagtccgatc gattgcccgt ttcgggtggg tgatgagcac
    15661 gttgaccgcc tctccaagca gacgaagatc atcatcgtgg attttcatgc ggaaaacacg
    15721 acggaaaaag aagcgctcgg tttttatctg gacggaaaag tcagctgcgt cgtcggaacg
    15781 cacacccacg tccagaccgc cgacgagaag atcctgccca aaggaaccgc ctatatcacc
    15841 gatctgggga tgtgcggacc gacgaagagc gtgatcggat cggatcccgt gctttcgatc
    15901 gcccgctcga tcagtcaaat gcccctgaaa agcgagatcg ccgatacccc gccgatgctt
    15961 cagggcgtca tcgtccggat cgaccccata accggcaaag ccctttcgat cgaacgcttc
    16021 acccgatgct tcagcgtatg aagaacgtgc cgcttctcca agcattgctc aaaacccatc
    16081 ccgcctatac caaagatgaa ttgcatgctt tcatcgtgtg caggaacgtc tatgtcgacg
    16141 gcgagttggt aagcgacccg aagcagggag tggatgaggg tgcgatcttt gaaatacaca
    16201 tccggaagta tgtgagtcgg ggcggataca agctggagca cgccctctcc tccttcaaga
    16261 tcgacgtatc gggcgaggtc ttcttggatg ccggctcttc aaccggcggg ttcaccgact
    16321 gtcttttgca gcacggggcc aagctggtcc acgccgtgga cgtgggatac aatctgatcg
    16381 actggaagct gcgcagcgac gagcgggtga tcgttcatga aaggcaaaac atcatgactc
    16441 tcgccgagcc gagtccccct ccgacggcgg cggtgtgcga cctttccttc cgctcgatcc
    16501 gcaaagcggc ggcgcacatc ctcgaccttg taggaaacac ctacctgatc gccctcatca
    16561 aaccgcagtt tgaaatggaa aaccctccac cctcgtttga cggtgtcatc gaagaccccg
    16621 gcctcattgt tcaggtggtc acgcagctct ttctcagtct ggccgaggag ggtgtgggag
    16681 tcttcgatct tgtggcaagc cccatcagag gagcaaaggg gaatatcgag ttccttgccc
    16741 tcctgaaaaa ggggcagggg ctctcactca aactcctcac tcatcaggtt gaagctcaac
    16801 ttgagctcct tcaataagga ctacgatatc cttcgccccg agcagtgaag cggtttcttt
    16861 aagttgacga tatgccagct ccatattcga tgaagcgaga aactcctttg aaaggttcag
    16921 gtagatgatg tttgaggaac gggtaaggcc caagagcttc gtgcccgatg cgatgtatgt
    16981 aatcgctccg tctttcaacg cggtcaacgg aactcccgag agcagcgcct caaaggtgtc
    17041 gtggcttgcc gacgatccca ggcgcggctg ctcgaccgta tagacggcga acgaggtgcc
    17101 aagcggagca aagacgacgc ttaccatccg tttggacaag gatgcttggg tttgttcctt
    17161 cggactccgg ataagctgaa gaaccccgct ttcatgaaag gcgcgcttta ccgtcgggaa
    17221 gctgacaatt ccgatgatcg tcgtatacag gatgagaagc aggaggatga taagtgcccg
    17281 aaggagggct tttgggtggt tggtcatcgc cttatcgtag caaagagtgg gctcttcttc
    17341 cagctttaca tcacggagca aactccgtat cataccgata tatgaggatt tttacgggtt
    17401 tggcaatcgg tgaaggaatc gctcggggta aggcaaaaat ctatcgccat accccgatcg
    17461 tcgttgaacg gaagcggatc gccaaatctc aggtgaaacg tgaacacgga ctcctcgatc
    17521 aagcgtttca acgcgcccgt acggctcttg agcgccgcaa aggtaatcat ttggaaggtg
    17581 aggcgctgga gattttggaa acccacctcc tcctgttgca agatccgacg tacatcacat
    17641 cgatcaagga gaagatcgaa gataagctca tcaacgccgc atgggccacc gaagaggtta
    17701 cagagggaat cgtcaagacg ctcgaggcaa gcgatgacga gctgtttcga cagcggacaa
    17761 tcgacatcaa cgatatcggt gactcgattg tcgaacacct ctgtggcaag agcgctcatc
    17821 cttccctggt tctcgatgaa ccggtgatcc ttctggcgga caccttgttg ccgagcgagc
    17881 tcttcagctt ggatcgctcg aacattctcg gtctgagcct ggatggggga gggctggcga
    17941 gtcatgtggc gattctcgcc cgggggttga atatccccac cgtcattgaa acgcagttgg
    18001 cctcccatgt cgccgaagat ggcgatgatg tgatcatcga cggcatccgg ggggaagtga
    18061 ttatcagggc ggaccatcag gcggttcaga acgttaccga acgctacaac gcctacctgg
    18121 aacacgaagc ggaactcctc aaaggagcga gcttggaagc ccgaaccaag gatggaaaac
    18181 ggattctcat caaatccaat atccagaccc tcggcgaact gccctttgcc gaggcggagg
    18241 gcagtgacgg gatcgggctc ttcagaacgg agtttttctt catggagcgt tcggctcttc
    18301 caagcgaaga ggagcagttc cggatctaca aggaagtgct tgagcgggtc gctccgagaa
    18361 gcgttacgat tcgaaccctt gacctcggag gggacaaggt cgtcagcggc ctcaacttgg
    18421 tggaagaaaa ccctgttctc ggtttcagag cggttcgttt ttgcctggag caccaagata
    18481 tattcttgac gcaaatgagg gcgattcttc gtgcttcccc gttcggaaaa gcggacatca
    18541 tgtttccgat ggtcagcggg attggagagc tgaaagagac gcttcgcctc tttgagctcg
    18601 ccaaacgaca actgaaggaa gagggagtga gcttcgatcc cgaccttcaa gtgggaacga
    18661 tgatcgaagt tccttccgcc gcgctgataa cggacctgat cgtccgacat gttgatttca
    18721 tttcgatcgg gacgaatgac ctgatccaat acacgatcgc ggtcgatcgg aacaaccaaa
    18781 aaatcgccca cctctaccaa ccccttcatc ccggggtgtt gcgcttgctc aaaggcgtcg
    18841 ttgatcaagc gcacgggatg ggaaaacgcg tagcggtatg cggcgagatg gcctccgacc
    18901 cccatcatgc ggttgtcctg gtgggtctcg gtgttgatga actgtctatg ggaccccacg
    18961 cgctcctgaa gctgcgtaag attgtccgat cactcgatct tgccgccgcc caaagcctgg
    19021 ttgagcatct cttgaccatg gactcatctg taagaactga acaagcagta cgggagtgga
    19081 tgcatgaacg attcgatgac cttactatcc gatgatccgg tggtggtttc cataatcggt
    19141 cgaccgaacg taggaaagtc gaccctcttc aaccgcctca tcggcaggcg ccgggcgatc
    19201 accgacccga cgcccggggt tacccgggat ctgctgccgg aacggtggta tctggcgggc
    19261 aaccccgtga ccctcgtcga cagcggggga gtgaagatgg acgggcaaac ccttgatgag
    19321 atggtcagtg ccaagagtct ctcactgctg aagcgaagcg atgcgatcat cttcatgatg
    19381 gattgcaccg aggtgaccgc cgaagacctg cacctgctcg aggcgctgcg cccctacggc
    19441 gatcggatgg ttctcactgt caacaaggtg gatgacccga agcgggagga cttggtttgg
    19501 gagttctacg gctacgggta tgagcgggtc gtcggcatca gcgccgccca cggtcttgga
    19561 atcgatgatt tggaagagct cctgctcggg atgctcgact ttgaagagat cgagcaaacc
    19621 gaggaggaag agacgaccgt caagcttgca atcatgggca aacccaacac cggcaaatcc
    19681 acgctggcga atcttctcgt cggaaggcag atctccctgg tcagcgaaat cgccgggacc
    19741 acccgcgacg tcgtaagcgg ctcattcgtt cacaagggca cgaccttcaa tgttctggat
    19801 accgccggaa tccggcgcaa aagcagagtg gacgaggata tcgagtacta ctcggtgaac
    19861 cgggcgatcc ggacgattga ggaggcggat gtcgtcttcc tcgtcgtcga ctccgccgag
    19921 ggcttggcgg atcaggacaa gaagatcgct cagctgatcg ttcgccgcgg aaaaggggtg
    19981 gtcatcgtcc tcaacaagat cgacctgctc tcgggtgttc ccaacgagct ggaagcaatc
    20041 agggaccgga tccgtttcct ttttcccgtt ttgaacttcg ccccggtggt gatgatcagc
    20101 gccctcgagg agaagggaat cgagccgctt cttgataccg cgtggagcct gtatcgtcag
    20161 ctccatcgaa gaatcgaaac atcccacctg aatgaagcgc tcagaaggtg gggcgaggag
    20221 tacccgccgc cccgcggccg ggaaggacac ttcaaggtct actacggaac gcaggtgtca
    20281 accaatccgg tgaacttcct cctcttcgtg aatcggatga aagaatttcc ggcttcctac
    20341 cttacctatc tgaccaacaa gattcgaagc gagctcggct ttcctcagat tcccgttgaa
    20401 atcgacctga aggagcggag acgttcgccc tcgctgaacc ttaaaggacc caagccgatt
    20461 caagatcgtg aagagaaacg ggaagtcgtt actcctagaa gaaccggcgg gaaggcggtc
    20521 gcccgaaaaa aaggggtgaa gccggggaac aaggcggcgg ttgaaaaagc ccgaaggcgg
    20581 gcgattaagg aacaacagca acgaaaaggc gggaaatgat gggctttatc gcggaagcag
    20641 gcattaccac gctcgccttg gatatcgacg gaaccctcta cagccgattc cagctcaata
    20701 cccggatgat tcgttccctc tttccgaatg tccgtttagc catcgcgttc aaccgggcgc
    20761 gcaaggaggt ccgtcgaatc caagatgaat atccgccgga tgatgaaaca cgagagggat
    20821 ttctcgatcg ccaggccgcg ttggtcgcag agcggtacaa aaaggacgtc ggtatggtaa
    20881 aagagctgat cggcacccag ttctatcagg catgggaacg aacctttaca accattactc
    20941 cgtttcacca tctccgcgaa acccttgaac aggtgaagag cgaaggggtt cgcatcattc
    21001 tcttctccga ctttcccatc gccgagaagc cgacgacgct cgggatcgcc gatcttgtcg
    21061 atgacgcatt caccagtgaa gagagcggct acctcaaacc gagtgtgaag gcgttcgagt
    21121 acctgatagg caaaagcgga gcggagcgat cgaagatcct gtatatcgga gacagctatg
    21181 acaaggactg tatcggagcc accgacgccg gactggtcag ctggtacctt ggaaagaaga
    21241 aggcgggacc gaaggcgagc aggtctttcg cttcgtggtc cgagatcaaa gcggtcatat
    21301 aggagggtgc actcatgggg cttgaaatca tactgtcgct gctgctcttt gtcatcacgc
    21361 tgatcatcat gctgctcttg cgggctcggg ataaacgcag tcattctctg gaagtgatga
    21421 agaatcggat ggggcagttc atcaaggaga tcgatgccac ccgttccctt tttcgggaag
    21481 agatgcaaag cgtcgaggaa cgcctcaact gggtgatcga tgaagccagg cgcggcagcg
    21541 aggcgctctc ttcccgcctt gacgagatcg atcggcggcg gagcgagctg atcgaactgg
    21601 aatcaattct cgcccgctat cgaaatactc ttctggagct tggggaaacg acccttgcga
    21661 tcgaccgcaa gatcgaagag caaaaaacgt ggcagaaaca gatcgatgat attcacgctc
    21721 aaatcgatga gttcgacttt cgtctgcagg gattcgcctc ctcattcgag cgggcgttga
    21781 agagcgcata caaggcgatg gaagaacctc ttcttacttg acgcttcaag caggggatga
    21841 gtatgttagt cgcgtgggcc gaatcggtcc catgctttta tatgcatttt attgtaatcg
    21901 gtaatacgag gacctgtaga tgagcaagaa ggaagaagcg aagaaaaaag aaacagtgaa
    21961 gaaggaagat acggaagagc ttgttgaaga tcttcagagc attgaagatg aacttgccga
    22021 ggctcgacgg gagatcgccg ctctgaaaga ggagaatctc cgcgaccggg cggatctgga
    22081 gaattggcgc aagcgaatgc tccgggacaa ggaggattcg atccgattcg ccaacgaacg
    22141 cctgatcatc gaccttctcg gaccgcttga tgatttcgaa cgagcgatag aagcggcgga
    22201 agaggcgcag gacttttcga aagtgcacga cggggtggtc ctcgtcagtg aacagatcta
    22261 ttcgacgctt cagaaaaatt ggggcttgga gcgaatcgaa gcggtcggaa aggagttcga
    22321 ccccaacgag catgaggcgt accaggtcgt tgaagatgaa aatcttgaac atgaaacggt
    22381 cctggaggag tatgccaccg gctataaact tcatggtcga gtgcttcgtc cctcaaaagt
    22441 gaaagttgga cgaccggcca tttgacgata aggccggtgt atgagtattt tacatacacg
    22501 gaatgaaaca gaattgatcc tgagttccgc agccaaaacc gaaaaagagg cttctaacta
    22561 attgcactgt t
//
LOCUS       DAOSLI010000031        22308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2582_length_22308_cov_26.148295, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000031 DAOSLI010000000
VERSION     DAOSLI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 22308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22308
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2582_length_22308_cov_26.148295"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..441
                     /locus_tag="PLF67_05845"
     tRNA            369..441
                     /locus_tag="PLF67_05845"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:402..404,aa:Lys,seq:ttt)
     gene            449..520
                     /locus_tag="PLF67_05850"
     tRNA            449..520
                     /locus_tag="PLF67_05850"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:482..484,aa:Glu,seq:ttc)
     gene            757..1815
                     /locus_tag="PLF67_05855"
     CDS             757..1815
                     /locus_tag="PLF67_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOE89571.1"
                     /translation="MNLQRLKTICDLGGASGFEDDVVFYLQEELKAIGPSSIDSMLNL
                     YVHRKEAKPDDPVVMLDAHTDEVGFMVRLIRPNGLIEFTTIGGWVTTNIPAHQVLVRT
                     IDGTYITGIVGSKPPHYMSESERNQAPAIENLYIDIGVSSQQEACDLGVRVGNPIVPK
                     AELEFKNNGIICGKAFDDRLGCAAVVEVLEAIRGESLSVNPVGVFSSQEEVGLRGAQV
                     AANTLKPDVAICFEGTPADDTFESGDRQQTVLGKGPMLRYIDSKMITNPRFQRFALDV
                     AKKYDIRVQTAVRTGGATNAGAIHLSGGGVPSIVIGVPVRYAHTHYSVASLDDLKGAI
                     ALGKAILKELNREIIASF"
     gene            1891..3249
                     /locus_tag="PLF67_05860"
     CDS             1891..3249
                     /locus_tag="PLF67_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOE89572.1"
                     /translation="METLRTCFTEMTAFFDRGESRSLAFRKTQLLSLRTALLEHEEEI
                     LEALSSDLQKSKVESWMTELGIVLEQIAYHLKRIDTWAKPQRVRTTLSTFPSSSYTIA
                     EPKGVVLIISPWNYPLQLTLVPLISAIAAGNCAIVKPSRQSRHTTLVIEKILSSIYPA
                     HYIAAVTEGDLLSLPFDHIFFTGSTRVGKLVMKSASEHLCSVTLELGGKSPTIVDASA
                     NLTLAAKRIIWGKTLNAGQTCIAPDYVLVERTILPRFLERMKDAMKNQWGLDPLNNPD
                     YPSIISHEAFDRLITLFEDGRIAYGGQIDPKRKRIAPTLLIDVDESSPVMEEEIFGPI
                     LPIIPFDAIDEALAFVKQRPKPLALYLFSQDRETQKKVTSELSYGGGCINDVVMHLAN
                     VHLPFGGVGQSGQGSYHAKEGFLSFSHVKPILKSRTFLDVPLRYPPYTGRRLKLIKRI
                     FS"
     gene            3264..4166
                     /locus_tag="PLF67_05865"
     CDS             3264..4166
                     /locus_tag="PLF67_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89573.1"
                     /translation="MKKIGALLLIGLFSTGVLFAALDFTTVDALYDSEQYSACKAGLL
                     SMRSQAATAQEEADVLWRLSRVTLGMGDDLDKKDKDGRFAAYEEGEEYANLSIARYPN
                     AYAYHWKASNIGRWGQTKGPLNALGKAKGMLEDLTMVVNDFNIDDYTETWYVLSSLYN
                     ELPGPPLSFGNNNWAISYMRRAMDTLPVHKLYPGHYKKLAEELWARNWSASKRGKELA
                     KMKTSWDKGGSTLERYRFYEGKDGGKSKPFYSSVTLDQMSDRQEAEMVLAYLEAKAKT
                     FTPVLPSEQRTLNEISELRASWLK"
     gene            4320..6407
                     /locus_tag="PLF67_05870"
     CDS             4320..6407
                     /locus_tag="PLF67_05870"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOE89574.1"
                     /translation="MPHPSHRKPDKEDCNDRSLARSWTFRGIDCPSCALGIEERLSKE
                     AGVDTVRLDYARKTIRITAKEEQSSSYFENLITIAKRIEPLFEVAHKKENTHHFPFEL
                     MRLIVSALFFILALVIREPWASIASYIVAGYPVIRRAIRNILGGKIFDEHFLMSVASL
                     GALAIGEVGEAAAVMLLYIIGEYLQEAAVEKSRNSILATLDLKTENARVIDGKNETVV
                     PGESVPVGTIVRVLAGEKIPLDGVVVSGTSTLDMRSLTGESMPLPVEKGSAVLSSSIN
                     ISGTLEIRTTKVWEESTAARILHLVEESSAKKAPTERFITTFARYYTPAVVASALLLF
                     LIPLIITGSHQPWLYRALVFLVVSCPCALVISVPLSYFAGIGKSAKSGIIVKGGNYLE
                     ALAKADCAIFDKTGTLTTGSFTIASIDLFDTDYSEEQVARLAASLERHSTHPLARAFA
                     EIPHARDVTDIQEHAGKGIIGTFGHQRIALGNNALYEVLEIPMEERGALLLSVDGFLV
                     ARFNVVDAIKDEAPALMKRLRSYGVATIGMISGDSKKNAEAVGLALGLDEVHAALLPH
                     QKQETMLEMGKRFPNYFYVGDGMNDAPALAAGKVGIAIGSGASDAAIEHADVVILRDD
                     LAEIGNLLSISRKTARIVRQNIALALGVKALAMIFAAFGYAPMWVAVIADTGVTALAV
                     LNALRLLIFNPSR"
     gene            6458..8488
                     /gene="fusA"
                     /locus_tag="PLF67_05875"
     CDS             6458..8488
                     /gene="fusA"
                     /locus_tag="PLF67_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE89575.1"
                     /translation="MNEQLIRNIGIMAHIDAGKTTTTERILYYTGESHRIGEVDDGEA
                     IMDWMEEEQNRGITITSAAITCSWKGHQINIIDTPGHVDFTAEVERSLRVLDGAIMVV
                     CAVGGVEPQTETVWRQADTYGVPRIAFINKMDRLGANFDEAVEELRKKLQANPIPLFL
                     PVGYENEFSGIIDVLNKTYLVYSPEDQGKTFTEQPIPEQYAEAAEAAYENVIDAVSSF
                     NDEITERFFEGKEIPVSMIKETIKKATIARQALPVFVGSSLKDIGVQPLIDGIIDYLP
                     SPSEIPPAQGIAAKSGDPIQVPFKPDGPPLALIFKIHVDREAGAMNYVRVYSGTIKKG
                     TALMNISRKKRERVNRIMRMYADRAEELSELKAGDIGVIIGFKEGRTGDTIGSEGAQI
                     LLEQIHFPVPVISIAIEPNTTSDGDKLRGALSILSQEDPTFSWRDDEETGQLIISGMG
                     ELHLEVLVNRLTKEMKIKARVGQPQVTYRESITKEASGSEEFSRIIANKEHTAGVTMT
                     VRPYRTGNGNRFINRANTRGIPHEFVEAVERGVTNAFMGGIRYGYETCNVEVEVTDIA
                     YNELTASTFAYEAASAMCFDKVANEANPVIMEPVMRMTIVVPTQYVGEAISSVTSRKG
                     IVTSIESRAFTEQIHAQAPLSELFGYSTVLRSATQGRGTFSLEFDHYQEKGV"
     gene            8615..8938
                     /locus_tag="PLF67_05880"
     CDS             8615..8938
                     /locus_tag="PLF67_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE89576.1"
                     /translation="MERTYTLQDIFARNLKERRRKLGLTQAKLAGKIGVSTSFITEIE
                     TSRKAPSFSTIEKIGRALEVPCWTFFCEDGDKFTGDVTVMDQFAYRLKQDIAKVIDQS
                     LSESR"
     gene            complement(8999..11419)
                     /locus_tag="PLF67_05885"
     CDS             complement(8999..11419)
                     /locus_tag="PLF67_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE89577.1"
                     /translation="MIALISNQESDAHIIKRALVPLEVVHFDRVSAGIDFLEEVEAPT
                     LLIVDLALGEEAKDLITLLVGNAFYAHIKVMVLCDTEEECESCLQRIVRPLSQRTVEP
                     LMGIYREFRQRHPGGRTERDDISYQALFTQVPLGIAVSKLIKRGNSQSHVIHQINPMF
                     EKILGRTKQEIMHLGIGGYTNAQDWEREQLLLKRLENRTITHYSLDKRCLRGDGKEIW
                     VQTLVFPLRFHKEDEFHYFGIIRDITEEKKLALKASEEVNQVLQEKLRGMGYRSHLDD
                     ELTVFHLTNGSYDLTGYTADELTAGQGFDYNKLLEPSSHRKVIDRLQEQIRIGKHARL
                     EYEIITKSGEHKWVFELGRLTEDDPPILEGIILDFSHYKELEQSRIYENERIPLTGLL
                     NRSALKRQMEEDKQNPEIQESALISINFTPLYESVSRLNHSYVEDLLVQIGSILAQFK
                     SETAWVYGPIDYSFAIYLSSYPSRSGLEALGTQIIGLVGNLLSSEHVGWGVGIIEIDW
                     SEEPEIDELLHQVIQASDLASKTHSGRPAIRFYERVISELIHRESSILRELSSIVEAV
                     DDDRLYLNYQPVVSVQTNEIVGFEALARLMSNELGLVGPSEFISIAEKYRLIIPLGYQ
                     ILTKALRFQKRLEERGCADYWVSINLSAFQLLEPGFANVVQSLMAEEGTNPEAIVFEH
                     TESGAYSSLTAVNSVLKTLQGFGSRIALDDFGVGYSSLARESELNVDYVKIDKLFSDQ
                     IIKLGADRSVLGDVIKMIHKVDRITIVEGVEEERQLDYLKQNGCILYQGFLYSKPVSE
                     EAALRLCT"
     gene            11774..13852
                     /locus_tag="PLF67_05890"
     CDS             11774..13852
                     /locus_tag="PLF67_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE89578.1"
                     /translation="MSVKSTDLRNVAIVGHNETGKTTLVEQMLAYAGVISRAETIESG
                     KTTSDYAEAEIANKISIYATLQSMTYGDKTFNIVDTPGTAGFIGETISGFRSTESAIM
                     VVDGRVGPQIETIKLWRNLDDRNKPRAVFINKMDRDHADFHKVLDDLKESFNVTFVPV
                     VIPVGASSEYKGVVNLIENKVYYYQPNGKETSGDIPAELEELVEEYRTELIESAAEGS
                     DELIEKYFEAGTLEPDEIRQGLQLGLRGNRLVPIFAGASGLGSGIIPLMNFIQNNFPD
                     PVGATDWSIDGEGNTHEFPITEEGPGSALVFKTTIDQFSGKLSFIKVLRGTIKGDTDL
                     TNPATGKRERPGKVYRMVGKKLNEVGSLVAGDVGVIAKSGITATNTSLVEGGDGSLIF
                     KPIAFPQPIYALAISSDDKKAEVKMNEALNRIAEEDLTFTIGFNEETKENVVAGQGEL
                     HLNMILDRVRDRQKVTINTKLPKVAYRETISKTSPICEYTHKKQSGGHGQYARVLIDI
                     KPLERGQYYSFENVIKGGAVSKGYIPGIEKGLHEQMEEGFLAGYPMMDIGITLHDGKE
                     HPVDSSEMAFKLAAKGAMRLALEKAGATLLEPIVSLSVFIDEEYLGDILSDLSGKRGR
                     VLGQEDIGHLILVKALVPQSELLNYAIDLKSMTSGTGSFEMEFDHYEPLSGKLADDVI
                     RAYKESQEEA"
     gene            complement(13948..14676)
                     /gene="trmB"
                     /locus_tag="PLF67_05895"
     CDS             complement(13948..14676)
                     /gene="trmB"
                     /locus_tag="PLF67_05895"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOE89579.1"
                     /translation="MNETIFKDFPELRVVDTREIGSDRKIKSYVLRTAGLKKREAKAI
                     ETYWPRYGLSFKEEMLDYSVLFGNDNPVVIEIGFGNGAATAHIAKMRSEYNYIGLEVF
                     LTGIAKLLNAVGKEGLDNIKMMRFDAVEVLKHMIADASIAGFHIFFPDPWPKKRHVKR
                     RLIQKPFAELLARKLIKGGYIYCVTDWQEYADQMLEVFDSIPGLTNPHGGFAPPIPWR
                     LKTSFEKKGIEKEHPINEVWVEKV"
     gene            complement(14687..17971)
                     /gene="mfd"
                     /locus_tag="PLF67_05900"
     CDS             complement(14687..17971)
                     /gene="mfd"
                     /locus_tag="PLF67_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOE89580.1"
                     /translation="MQTPITTSVQSYIKSGSAYKAYRGSETRLHVEGLEGYPLAIFLK
                     QITKIGNQRLWVVAPTEEVARTLLLDLGLALDPKSGDLIKRTAQDMKIHYLPSTGRTL
                     YAEKEIDNVEYEQANRLSEILSLKHGMIITTLRTFVSPLITPASLKRSELELKRGGVF
                     DPVLIARQLAEGSYLQVPFTSAMGQFSVRGEVLDIFPFEGARPYRIYADWDKIGRISA
                     FDPITQETIETVGSVSINILHEGGTIEYASIKEYLQKRDALAFIGDERLLTSYKSLQS
                     EAKSLYRTAFQIDRNVPKPNDLLLDYPLFVEENPAVIICDITRRSGGNHRFDFDGPRS
                     YFGNFTLLKDDLKALLRGGWKPTIFASNTLQRQRLEQMLSSIEGLAFEERELSGGFGI
                     PSLQVLAICEHEIFGRRREVVKTLRHTQTSPLDSFVDLNEGDYVVHVNYGIGRFEKID
                     RVSSFDRERDFIKIVYADRENLYVPIEQANLIQRYIGSDGSPPRLDKLAGMGWETKKA
                     KARKNAEDLAKHLITLYAKRMNSVGYAFSKDTDWQLQFEASFAFDETADQLQCIEEIK
                     EDMEKPIVMDRLVCGDVGYGKTEIAFRAAFKAVMDGKQVAFLAPTTILAEQHYRTFMK
                     RVGEFPVRSALLSRIVPPKRQKQILVEVAEGKVDVLFGTHRILQKDLLYHNLGLLIID
                     EEQRFGVKDKERIKTLRTNIDSLTLSATPIPRTLYMSLLKIRDMSLLATPPIERRAVE
                     TFIDGYDEYLVAKAIRRELERDGQVFYLHNRVESLPRVADRLRELVPEAIIDVAHGQM
                     EAAALEDQMRRFIHQGIQVLVSTTIIENGIDIPNVNTIIIDRADRYGLAQLYQLRGRV
                     GRSDRQGYAYLFYPPDSSLNELAVKRLRIISELTELGSGFKIALKDMEMRGTGNLLGR
                     QQSGQVASVGLDMYIRILDEAIRTLQNEGEKEEDRQVLLELDYTGFIPDSYIKTPSVK
                     FEVYRKISSIQNDEQLDALRTELADRYGQVPEEVENLLYIARIKILCRKLSITTLSDR
                     RGVVRIEFGKVADLNIDKVMNLIALSNKKVRLDRNVLNIMYIEVETVSLKDKALFLLE
                     HLERLL"
     gene            18016..18816
                     /locus_tag="PLF67_05905"
     CDS             18016..18816
                     /locus_tag="PLF67_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOE89581.1"
                     /translation="MKNEFLDLLAEEVVRCGTYAKERQGSVLRSFKSDGTVITEVDTT
                     ISERLTAFIKAHLPLANIVSEEAIDPFDEEAPYTFVIDPIDGTDGYSQGLPTWAVALG
                     ILDKKRNPVGAMISAPRMGIGVDNLFIRLDPGKELLINGKPFFPNPHKDVIHQVAMAS
                     SGHRLCDFTNFHGKIRVFGSAILHLLAPVIYPEIQGAMNSSVYVWDFCAAHAVLCHFD
                     YRLLYEDGRPVTYTDRLLKERKNTGEFIFAGTINGVVDLQHKIKVVPR"
     gene            complement(18797..19495)
                     /locus_tag="PLF67_05910"
     CDS             complement(18797..19495)
                     /locus_tag="PLF67_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOE89582.1"
                     /translation="MNELFWFGMLLVNFVFILTAFRLWGKTGLFAWIPVSVIVANIQV
                     TKNVTLFGLEATLGNIVYATSFLATDILSEFFGKKEAYKAVGIGFFSLVAMTVLMQLA
                     LVFTPSPADMVHDSMLAIFSLMPRIALASLIAYLASNLHDVWAFAYWKEKRPGRKTLW
                     MRNNFSTVVSQLIDTVLFTAIAFLGVYPRGVLLQIGISTYLLKWIVAVLDTPCIYLAR
                     SWVDRGYIPTGEQL"
     gene            19775..20002
                     /locus_tag="PLF67_05915"
     CDS             19775..20002
                     /locus_tag="PLF67_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOE89583.1"
                     /translation="MTTLNKVNAGRKVVVSDVKGDQRFLSRITSVGLTIGCELEILHN
                     EKKLPLLFYGRDTVVALNREESKNVFVEVIR"
     gene            19999..22041
                     /gene="feoB"
                     /locus_tag="PLF67_05920"
     CDS             19999..22041
                     /gene="feoB"
                     /locus_tag="PLF67_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOE89584.1"
                     /translation="MNAATITGHPTIALLGQPNSGKSTLFNGLTGARQRVGNWPGKTV
                     EKKEGYYVRDGIRYRVTDLPGTYSLSANSEEEIITRDYIASGEADVVCILADSSQLSR
                     SLFMLADYAGIECPAVLLLNLVDVAEAQGKTVDASKLEDKLGIPVIPFVAADRKQYGA
                     FFETVDRALKEKKRININSLTAKYYTIGNGMYEKLLALMPEEGIGNYSAPWLATKLIE
                     GDTAILDKVKNTLETEAYRDVQTILSSIQNGALHTSGCKFQWVDELLSDVLIQKQEKP
                     LFSKFDRLATHRRWGKPLAVFIILLGLVASFIPATPIMVLGSMIPRLGGPIAEILSSI
                     GVSQILIDLINQVVLNTLYFAVAMIGFVLGVNLVFGFLEEVGYMARISYVFDGTMAKL
                     GLQGKSVMPLVVSFGCTIGGAAGTRVIDSWGQRVLTIALAWVVPCAATWAIVPVISSL
                     FFGAWAPLIIVAIFIVAGLHMLITAKIFGSKLIREEDRAGLIMELPPYHKPRWKALLR
                     SIFVRTWDILARALRVIFLVSLAFWLLTYSSLGNPDASLLYRFGRAIEPVTIIFGLTW
                     QTFLAYFASMISKEAALGVLGSLYLGAGSLFTATIGSGGKVAVGLESVLLASLTAPAA
                     LAFIFAVTFNAPCLMAITSTYQETRSLKWTLRILGYYFSTSLILAFLVYRIANLFM"
BASE COUNT         5295 a         5982 c         5861 g         5170 t
ORIGIN      
        1 catccgatgc tgacaatttt cattgccctt catcttcgaa attgactgga agcccccttg
       61 cgggtgcatt ccctctctca tttccttccc tacgctaaac tctcaaaaaa cgccgactct
      121 gagacagagc cgacaggaac gcatcataac ctttttgatt gagtgtgtcc agtgggtcct
      181 gatgaatatg gcgattttga ggatttcaag gctttcagcg tcaaatcttc gcaaaatccc
      241 catttcatct tcttttcggc aacttcatgt ggaatcctat gtattacaat aacttaatca
      301 ctgattgccg attgcttaaa aggtgttgac accctcaacc tgttattgat atagtccaat
      361 cgttgttggg gccgctagct cagctggtag agcaacgccc ttttaaggcg tgggtcacag
      421 gtccgaatcc tgtgcggctc aataacgtgt ctccttcgtc tagtggttag gacaacgggt
      481 tttcatcccg tcaacagggg ttcgatcccc ctaggagatg ttttagaggg tttccatcag
      541 tggaagccct cttttcttta ccctgaactc ttgacttatc ggcactctgg ctttacaaat
      601 aaagatgcag gtggtggctg tcccaatagg cgtatatctc ccaaccagcc ttggccactg
      661 cctcttgcac ccttcttgat tgcaatccaa aggatccatc cacacccgct ttcctttggg
      721 ttgcagtctt tttattatcg ttacggaggt gaatccatga acctgcagcg gctcaagacg
      781 atttgtgatc ttggcggagc ttccggcttt gaagatgatg tcgtcttcta tcttcaggag
      841 gagctcaaag cgatcggtcc ttcatcaatc gactcgatgc tcaaccttta tgttcatcga
      901 aaggaagcga aaccggatga tcccgtcgtg atgctggacg cccataccga cgaggtgggg
      961 ttcatggtcc gcctcatccg accgaacgga ctcatcgaat tcaccacgat cgggggttgg
     1021 gtgaccacca atattcccgc ccatcaggtt ctggtccgga cgatcgacgg tacctacatc
     1081 accggcattg tcggctcgaa gccgccccac tacatgagcg agagcgaacg caaccaagcc
     1141 ccggcaatcg aaaaccttta tatagacatc ggagtgtcct cccaacagga agcctgcgac
     1201 cttggcgttc gagtcggcaa tccgatcgtt cccaaagcgg aacttgaatt caaaaacaat
     1261 ggaatcatct gcggcaaagc gtttgacgac cgccttgggt gtgctgcggt cgtcgaggtg
     1321 ttggaagcga ttcgcggaga atcactgtcg gtcaacccgg tcggggtctt ctccagccag
     1381 gaagaggtcg gactccgagg agcccaagtc gccgccaata cgctgaaacc ggacgtggcg
     1441 atctgttttg aaggaacgcc cgccgacgac accttcgaaa gcggcgaccg ccaacagacg
     1501 gtcctcggca aaggaccgat gctccgctac atcgactcga agatgatcac caatccccgc
     1561 ttccagcgct ttgccctcga tgttgcaaag aaatacgaca tccgggttca aaccgccgtt
     1621 cgcaccggag gagcgacgaa tgcgggagcg atccacctga gcggtggggg cgttccctcg
     1681 atcgtcatcg gtgttcctgt ccggtatgcc cacacccact attcggtggc gagcctcgac
     1741 gacctgaaag gagcgatcgc ccttggcaaa gcgattctca aagagctgaa ccgagagatc
     1801 atcgcttctt tttaacaatt ctttggataa tgtcgaaata aacggtctgg cgttttgaca
     1861 aacccgcttc aacggtgaga tactgctctc atggaaacac taagaacctg tttcaccgaa
     1921 atgaccgcct ttttcgatcg gggagagagt cgctcccttg cgtttcggaa aactcagctg
     1981 ctttccttga gaaccgccct gcttgaacac gaagaagaga ttctcgaagc gttatcgtcg
     2041 gacttgcaga agtccaaagt cgaatcatgg atgacggagc tcggaatcgt tctggaacag
     2101 atcgcctacc atctgaaacg gatcgatacg tgggccaaac cgcaacgggt tcgaacgacc
     2161 ctctccacct ttccctcctc ttcctatacg atcgccgaac cgaagggcgt ggtgctcatc
     2221 atcagtccct ggaactatcc ccttcaactt acgctggtac ccctgatcag cgcgatcgcc
     2281 gccggcaact gcgcgatcgt gaaaccctcc agacagagca gacataccac cctcgtcatc
     2341 gaaaagatcc tttcttccat ctacccggcc cattacatcg ccgccgttac cgagggagac
     2401 ctcctttccc ttccgttcga ccatatcttc ttcaccggtt cgacccgggt cggcaagctc
     2461 gtcatgaaga gcgcaagcga acatctgtgt tccgtcacct tggagcttgg agggaaaagc
     2521 ccgacgatcg tcgatgcgtc cgccaatctc actctcgccg ccaagcggat catctgggga
     2581 aagacgctca atgccggaca gacctgcatc gcccccgact acgtgctggt cgagcgaacg
     2641 atccttcccc ggtttctcga acggatgaag gatgcgatga aaaaccagtg ggggttggat
     2701 cccctgaaca acccggatta tccctcgatc atctcccatg aagcattcga tcggctcatc
     2761 accctctttg aggatggcag gatcgcctac ggcggtcaga tcgacccgaa gcgcaaacgg
     2821 atcgccccta ctctcttgat cgatgtggat gaatcctctc cggtcatgga agaggagatc
     2881 ttcggaccga ttctcccgat catccccttc gatgcgatcg acgaggcgct tgccttcgta
     2941 aaacaaagac cgaaaccgtt ggccctgtat ctcttcagtc aggacagaga gacccaaaag
     3001 aaggtcacaa gcgagctctc ctacggcggt ggctgcatca atgatgtggt gatgcacctg
     3061 gctaacgtcc accttccctt cggaggagtc gggcaaagcg gccagggctc ctatcacgcc
     3121 aaggaggggt ttttatcctt cagccacgtt aaaccgattc tcaaaagccg caccttcctt
     3181 gatgttccac tgcgctatcc cccctatacg gggcggagat tgaaactcat aaaacggatt
     3241 ttttcgtaac tagggaggaa tgtatgaaaa agattggagc attgctactc attggccttt
     3301 tcagtaccgg tgtgctgttc gccgctttgg atttcacaac ggtcgatgcg ctctatgaca
     3361 gcgaacagta cagtgcatgc aaagccgggc tcttgagcat gcgctctcag gccgcaaccg
     3421 cccaagagga agcggatgtg ctgtggcgcc tctcccgggt gacactcggc atgggggacg
     3481 atttggacaa gaaggataaa gacgggcgct ttgcagcgta tgaggaaggt gaagagtatg
     3541 caaacctttc catcgcccgc tatcccaacg cgtatgccta ccactggaaa gcgtccaaca
     3601 tcggtcgatg gggtcaaaca aaaggtcccc tcaatgccct tgggaaagcg aaaggaatgc
     3661 ttgaagacct gacgatggtc gtcaatgact tcaatatcga tgactatacc gaaacatggt
     3721 acgtcctttc ttcgctctac aacgaactcc ccggtccgcc cctttccttc gggaacaaca
     3781 actgggcgat cagctacatg cggcgggcga tggatacgct cccggtgcac aaactgtacc
     3841 cgggacacta caagaagttg gcggaagagc tgtgggcccg aaactggagc gccagcaagc
     3901 gcggcaagga actggccaag atgaaaacga gctgggacaa aggcggttcg accttggagc
     3961 gctatcgctt ctacgaagga aaggacggag gcaaatccaa acccttttac tcctccgtca
     4021 ccttggatca gatgagcgac cgccaggaag cggagatggt cctcgcctac cttgaagcca
     4081 aggcaaaaac cttcacccct gttttgccca gcgaacagcg caccctcaat gaaatttccg
     4141 aactgagggc ttcttggttg aaataacagc cttcaaccga agatcgatat ccggccggcg
     4201 attgccggcc ggtgttcttc atgtcattat ctcctaattt cttccattcc tgttgacagg
     4261 aaagagattc ctttctaatg tatgaataca taatcatata atgattaatt ggaggggata
     4321 tgcctcatcc gagtcacagg aagccggata aggaagattg caatgaccgt tcccttgctc
     4381 gatcatggac gtttcgggga atcgattgcc cctcctgtgc ccttggcatt gaagaacgat
     4441 tgagcaagga agccggcgtg gataccgtgc gccttgacta cgcaaggaaa acgatcagaa
     4501 ttaccgccaa agaagaacaa tcctcctctt atttcgagaa tctcatcacc atcgccaagc
     4561 ggattgagcc cctgtttgaa gtggctcaca aaaaagagaa tacccatcat tttccctttg
     4621 agttgatgcg gctcatcgtt tccgccctct tcttcatcct cgccttggtc atccgagaac
     4681 catgggcatc catcgcgagc tacatcgtcg ccggttaccc ggtaatccgg cgggcgatcc
     4741 gaaacattct cggcggcaag atctttgatg aacactttct gatgagcgta gcgagcctcg
     4801 gagcgctggc gatcggagag gtcggcgaag cggccgccgt catgctgctt tacatcatcg
     4861 gcgaatatct tcaggaagcc gccgtggaaa aaagtcgaaa ctcgattctt gcgactcttg
     4921 atctgaaaac cgagaacgct cgggtcatcg acggaaagaa cgaaaccgtc gttcccggtg
     4981 aatccgttcc cgtcggaacg atcgttcggg tcttggccgg cgaaaagatt ccgctcgacg
     5041 gtgttgtcgt cagcggaacc tcgactctgg atatgagaag cctcaccgga gaatcgatgc
     5101 ccttgccggt tgagaaagga agcgccgtat tgagctcttc gataaacatc agtggaactc
     5161 tggagatccg gacgaccaag gtctgggagg aatcgacggc ggcccgaatt ctccatctcg
     5221 tggaggagtc ctcggcgaag aaagccccga ccgagcggtt catcaccacg ttcgcccgct
     5281 attacacccc ggctgtcgtc gcttcggccc tgctgctctt cctgattccg ctcatcatta
     5341 ccggcagtca tcaaccgtgg ctctatcggg cgttggtctt tttggtggtc agctgcccct
     5401 gcgctctggt catcagcgta ccgctttcct actttgccgg aatcgggaaa agcgcgaaga
     5461 gcggaatcat cgtcaagggc ggcaactacc ttgaagcgct ggccaaggcg gattgcgcga
     5521 tcttcgataa aaccgggacc ctcaccaccg gatcgttcac gatcgcctcc atcgacctct
     5581 ttgacacgga ttattccgaa gaacaggtcg ctcgcttggc cgcatcgctg gaacggcact
     5641 ccacccaccc gcttgcccgg gcgtttgcgg agattcccca tgcccgggat gttaccgaca
     5701 ttcaggaaca tgccggcaag gggatcatcg gaaccttcgg acatcaacgg atagccctcg
     5761 gaaacaacgc cctgtatgaa gtgctggaaa tccccatgga ggaacgagga gcgcttctgt
     5821 tgagcgtcga cggcttcctt gtggcccgct tcaacgttgt cgatgcgata aaggacgaag
     5881 ctcccgctct gatgaagcgc cttcgatcct atggcgttgc cacgatcgga atgatcagcg
     5941 gtgacagcaa aaagaacgcc gaagccgtgg gtctcgccct tggattggac gaggtccatg
     6001 cagcgctgct gccccaccaa aaacaggaaa cgatgcttga aatgggaaaa cggttcccca
     6061 attatttcta tgtcggtgac gggatgaacg acgccccggc tctggcagca ggaaaagttg
     6121 gaatcgcgat cggcagcggc gccagcgacg cggcgattga acacgccgat gtggtcatcc
     6181 tccgcgacga tctggcggag atcggaaatc tgctttcgat cagcagaaaa accgcccgga
     6241 tcgtcaggca gaacatcgcc ctcgccttgg gggtgaaagc cctggcgatg atctttgccg
     6301 cctttggcta tgccccgatg tgggttgcgg tcatcgccga tacgggagtg acggctctgg
     6361 cggtcctcaa tgcattgagg ctgcttatct tcaatccttc acgttgattc tccctctttt
     6421 gtatggtatc tttgcaacgc tttacaagga ccacgcgatg aacgaacaat taatccgaaa
     6481 tatcgggatc atggcccaca ttgatgccgg aaagacgacc accactgaac gaatcctcta
     6541 ttacaccgga gaaagccacc ggatcggtga agtcgacgac ggtgaagcga tcatggattg
     6601 gatggaggag gaacagaacc ggggaatcac gatcaccagc gcagcgatta cctgctcgtg
     6661 gaaaggtcat cagatcaata tcattgacac gcccggccac gtggacttca ctgccgaggt
     6721 ggagcgctcc ctccgcgtgc tcgacggggc gatcatggtg gtgtgcgccg ttggcggggt
     6781 cgagccccag accgagaccg tctggcgtca ggcggatacc tacggagttc cccggatcgc
     6841 cttcatcaac aagatggacc ggctcggagc gaacttcgac gaagcggttg aagagctgag
     6901 gaaaaagctt caggccaacc cgattccgct ttttctgccg gtgggatatg agaacgaatt
     6961 cagcgggatc atcgatgtgc tgaacaagac ctacctggtg tactccccgg aagaccaggg
     7021 aaaaaccttc accgagcagc cgattccgga gcaatatgcc gaagcggcgg aagccgcgta
     7081 tgagaacgtg atcgacgcgg tctcctcgtt caacgacgag atcaccgaac ggttttttga
     7141 aggaaaagag attcccgttt ccatgatcaa agagacgatc aagaaggcga cgatcgctcg
     7201 gcaggccctg ccggtcttcg tcggctcctc gttgaaagat atcggggtgc aacccctgat
     7261 cgacggcatc atcgattacc ttccttcccc gagcgagata ccgccggctc agggaatcgc
     7321 ggcaaagagc ggggatccga ttcaagtccc cttcaaaccg gacggccctc cccttgcctt
     7381 gattttcaaa atccatgtgg atcgggaagc gggagcgatg aactacgttc gggtctacag
     7441 cggtacgatc aagaaaggca ccgccttgat gaacatctcc aggaaaaagc gcgaacgggt
     7501 caaccggatc atgcgcatgt acgccgaccg cgctgaagaa ttgagcgaat tgaaagcggg
     7561 cgacatcgga gtgatcatcg ggttcaaaga aggccggacc ggggatacga tcggtagtga
     7621 aggagctcag atcctgctgg agcagattca tttccccgtt ccagtcatct caatcgcgat
     7681 cgagccgaac acgacgagcg acggtgacaa gctgcgcggc gctttaagca tcctcagcca
     7741 ggaggatcct accttctcct ggcgggatga tgaggaaacc ggccaattaa tcatcagcgg
     7801 aatgggcgag ctccacctcg aagtgttggt gaaccgcctg accaaggaaa tgaagatcaa
     7861 agcccgagtc ggccagcctc aagtcaccta ccgcgaatcg atcaccaagg aagcttcggg
     7921 cagcgaagaa ttcagccgaa tcatcgccaa caaagaacac accgccggcg taacgatgac
     7981 ggtccgcccc taccgcacgg gaaatggaaa ccgcttcatc aaccgggcga acacccgcgg
     8041 cattccccat gagtttgtcg aggcggttga acggggagtt accaacgcat tcatgggagg
     8101 aatccggtat gggtacgaaa cgtgcaacgt tgaggtcgaa gtgaccgata tcgcctacaa
     8161 tgagttgacc gccagcacct tcgcctacga ggcggcgagc gcgatgtgtt tcgacaaggt
     8221 cgcaaacgaa gcgaatccgg tcatcatgga accggtcatg cggatgacga tcgtcgttcc
     8281 tacccaatat gtcggcgaag cgatcagcag cgttaccagc cggaagggaa tcgtcacctc
     8341 gatcgaatcg cgggcattca ccgaacagat tcacgcccag gctccgctgt ccgagctctt
     8401 cgggtattcg acggtcttgc gttcggcaac gcaaggaaga ggcacattct cattggaatt
     8461 tgatcattat caggaaaaag gtgtataatg tttctatata accggtttca cccttgcgaa
     8521 ttcgcattat tcgtgtatga atatacgata gtggatcggt taatgtaaca ttttcgtttt
     8581 ttatttgttt gaacagctga agaaggagta caacgtggag cgaacataca cattacagga
     8641 tatttttgcc cgcaatttga aggagcgacg gcgaaaatta gggttgaccc aagccaaact
     8701 tgctggaaaa atcggggttt ccacctcatt catcaccgaa attgaaacct cccggaaagc
     8761 cccctccttt tccacgattg aaaagatcgg tcgagctctc gaagtcccct gctggacgtt
     8821 cttctgtgag gacggggata agttcaccgg tgatgtcacc gtgatggatc agtttgcata
     8881 ccggctcaaa caggatattg caaaggttat tgaccagtca ttgagtgaaa gtcggtaact
     8941 accgaaaaaa aagtgcaaag gggccggtgc aaacgttgaa catgcgacgg cctctttttt
     9001 atgtgcacag cctgagagcc gcttcttcgg aaaccggttt tgaatagagg aacccttgat
     9061 agaggatgca accattttgt ttcaagtaat ccaactgccg ctcctcttcc actccttcaa
     9121 caatcgtgat gcggtccact ttgtgaatca tcttgatcac atccccgaga acgcttcgat
     9181 ccgctccgag cttgatgatt tgatctgaaa agagtttatc gatctttaca taatcgacgt
     9241 tcaactcgct ctcccgagcc aacgaagaat aaccgacgcc gaaatcatcg agggcgattc
     9301 gcgatccgaa accttgaagc gttttgagaa ctgaattgac tgcggtaagg gatgagtaag
     9361 caccggattc cgtgtgctca aacacgatcg cttcggggtt cgtgccctct tcggccatca
     9421 gcgattgcac gacgttggcg aaccccggct caaggagctg aaatgctgaa aggtttatcg
     9481 aaacccagta gtcggcacat cctctttctt caaggcgctt ttggaatcga agtgctttgg
     9541 tgaggatttg atatccaagg ggaatgatga gccggtattt ctcggcgatg gaaataaatt
     9601 cactcggccc cacaagcccc agctcattgc tcatcagccg ggcaagcgct tcaaagccga
     9661 cgatttcatt cgtttgcacg gaaaccaccg gttgatagtt caggtacaag cgatcatcat
     9721 ccactgcttc gacaatcgaa gaaagctccc gcaggatgct gctctcacgg tggatcaatt
     9781 cgctgatcac ccgctcataa aaacgaatcg ccgggcggcc ggaatgggtt ttggaggcca
     9841 aatcactggc ttggatcacc tgatggagaa gttcatcgat ctccggctct tcactccagt
     9901 cgatttcaat gattccgact ccccagccga cgtgttcgga agagagcaga ttaccgacaa
     9961 ggccgatgat ctgggttccg agcgcctcaa gcccgcttcg gcttggatat gatgaaagat
    10021 agattgcaaa gctgtagtcg atcgggccgt acacccacgc ggtttcgctc ttgaactggg
    10081 ccaggatcga cccgatctgc accagaaggt cttccacata agagtggttg agtcgggaga
    10141 ccgactcata gagaggtgtg aaattgatgc ttatcagagc gctttcctga atctccggat
    10201 tctgtttatc ctcttccatc tgtcgcttta aagcgcttcg attcagcagg ccggtcagcg
    10261 gaatacgttc gttttcgtag attcgactct gttccagctc tttgtagtgg gaaaaatcca
    10321 gaatgattcc ttcaagaatc ggcggatcgt cttccgtcag ccgccccagc tcaaacaccc
    10381 atttatgctc tccgcttttc gtgatgatct catactcgag gcgggcgtgc ttgccgatcc
    10441 tgatctgttc ctgaagccga tcgattactt tccggtgaga gctcggctca aggagcttgt
    10501 tataatcgaa tccttgaccg gcggttaatt catccgctgt atagccggta aggtcatacg
    10561 acccgttggt aagatggaag acggtaagtt catcgtccag atgggaccga taccccatcc
    10621 cccgcagttt ttcctgcaga acctgattca cttcctcgct tgctttcaga gccagttttt
    10681 tttcttcggt gatatcccgg ataatgccaa aataatgaaa ttcgtcctct ttatgaaacc
    10741 gaaggggaaa gaccagcgtt tgaacccata tttccttccc atcgccccga agacatcgct
    10801 tgtcgaggga gtaatgggta atcgtccgat tctcaagccg tttcaagagc agttgctccc
    10861 gctcccagtc ctgtgcgttt gtatatcccc caatccccag atgcatgatt tcctgctttg
    10921 ttcgtcccaa aatcttttca aacatcggat tgatttgatg aattacgtga ctttgggagt
    10981 ttccgcgctt gatgagcttt gaaacggcga ttccgagggg aacttgcgta aacagcgctt
    11041 ggtaggagat gtcatcccgt tcggtccgtc cacccggatg gcgctgccgg aattcccggt
    11101 agattcccat gagaggctca acggttcttt gagagagcgg acgaacaatc cgctgaaggc
    11161 aggattcgca ttcctcttcg gtatcgcaga gaaccatcac cttgatatgg gcatagaatg
    11221 cattcccgac caaaagcgtg atgaggtctt ttgcttcttc gccgagggca aggtcaacga
    11281 tgagaagtgt gggagcttcc acctcttcaa ggaagtcaat ccctgctgaa acccggtcga
    11341 agtgaacaac ctcgagagga acaagcgcac gtttaatgat atgcgcatca ctttcctgat
    11401 tacttatcaa cgcaatcatc cggttccctt tatcgttcag tcttcgaaaa cggcacctat
    11461 gtcaaatgta ccacaccata ttggccaaca ccacattttg tcgacatttt ttttcaacaa
    11521 gcacatcaac atactgaacg acgcatgaag aaagagcatc ttgagggaga catgcgcatc
    11581 gattgcatga caaatggcat cgttcatcgc ttcgatcttc tttcaaagta tggaccatcg
    11641 aattgtttgt ggtgtaggat actagacgca actctctgca tgaacaaaag aaaccttttg
    11701 acaggtgcca ccaaaggagc gtaccatacc ggtatgcgtt ttggattatc aattggtgat
    11761 cgaaggagga tctatgagtg ttaaaagcac ggacttgagg aatgtcgcca ttgttggcca
    11821 caatgaaacc gggaaaacaa ccctcgttga gcaaatgctt gcctatgccg gggttatttc
    11881 ccgggcagaa acgattgaaa gtggaaagac caccagtgac tacgcagagg cggagatcgc
    11941 caacaagatt tctatctacg caacgctcca gtcgatgacc tacggggaca agacgttcaa
    12001 catcgtcgac accccgggaa cagccggctt cataggcgaa acgatcagtg gattccggtc
    12061 caccgaatcg gcgatcatgg ttgtcgatgg ccgcgtcggt ccgcagatcg agacgatcaa
    12121 gctttggcgc aatttggatg accgaaacaa gccgcgggcg gtgttcatca ataaaatgga
    12181 tcgggaccac gccgattttc ataaagttct tgatgacctg aaggagtcct tcaacgtcac
    12241 cttcgttccg gtagtgattc cggtcggagc cagcagtgaa tacaaagggg tcgtgaacct
    12301 cattgaaaat aaagtctatt actaccaacc aaacggaaaa gagacaagcg gtgacatccc
    12361 cgccgagctg gaagagttgg ttgaagagta tcgcaccgag ctgatcgaat ccgccgccga
    12421 aggttcggat gagctgatcg aaaagtactt tgaagcggga acgcttgaac cggatgaaat
    12481 tcgtcaaggc ttgcagctcg ggttgagagg aaaccggctg gttccgatct tcgccggcgc
    12541 ctcgggcctc ggttcgggga tcattccgct gatgaacttc atccagaaca acttccccga
    12601 cccggtggga gcgaccgact ggagcatcga cggcgaagga aacacccacg aatttcccat
    12661 caccgaagag ggaccgggct cggcgctcgt cttcaagacg acgattgacc agttcagcgg
    12721 aaagctttcc ttcatcaagg tgcttcgggg aacgatcaag ggtgataccg acctcaccaa
    12781 cccggcgacc ggcaagcgcg aacgccccgg caaagtgtat cgcatggtcg gaaagaagct
    12841 gaatgaagtc ggttctctgg tcgccggaga tgtcggagta atcgccaaga gcggcatcac
    12901 cgcaaccaat acctcgctgg tggagggcgg agacggatcg ctcatcttca agccgatcgc
    12961 cttcccgcag ccgatctatg cgcttgcgat ctcctccgat gacaagaaag cggaagtgaa
    13021 gatgaacgaa gcgttgaaca ggatcgccga ggaagacctc acgttcacga tcgggttcaa
    13081 tgaagagacg aaggaaaacg tcgttgccgg ccagggtgaa ttgcacttga acatgatcct
    13141 cgatcgagtg cgggatcggc agaaggtcac gatcaacacg aagcttccga aagtcgccta
    13201 tcgggagacg atcagcaaga cgagtccgat ctgtgaatat acccacaaga aacagagcgg
    13261 cggccacggt cagtacgccc gagtcctcat cgatatcaag ccgcttgagc ggggacaata
    13321 ctactccttt gagaacgtca tcaaaggtgg cgccgtttcc aagggatata tccccggaat
    13381 tgaaaaaggc ctccacgagc agatggaaga gggattcctc gccggctatc cgatgatgga
    13441 tatcggcatc accctccatg acggtaagga acaccccgtc gactcgagtg aaatggcgtt
    13501 caagctcgcc gccaaaggcg cgatgagact ggctttggag aaagcgggag cgaccctgct
    13561 tgaaccgatc gtttccttgt cggtcttcat cgacgaggag tatcttggag atatcctttc
    13621 cgacctctcc ggcaaacgcg gccgggttct cggacaggaa gatatcggtc acctgattct
    13681 tgtaaaggct ttggtgccgc agagcgaact gctcaactat gcgatcgacc tgaagtccat
    13741 gaccagcggg accggaagtt ttgaaatgga gtttgaccac tatgagccgc tttccggcaa
    13801 gttggcagac gatgtgatcc gcgcctacaa ggagtcccag gaggaggcgt aaggatcgag
    13861 aatccgggac aatcagaagc tcactacatt tctgattgca tgagagtcaa tttgtaggac
    13921 ttgggagggg ttcgcccctc ccttttctta aactttctca acccacactt cgttgatcgg
    13981 gtgttctttc tcgatccctt tcttctcaaa agaggtcttc aggcgccagg gaatcggagg
    14041 ggcgaaccca ccatgggggt ttgtgagacc gggaatggag tcaaatacct caagcatctg
    14101 atcggcgtac tcctgccaat cggtaacgca gtagatatac ccgcctttga tgagcttccg
    14161 ggccagcagc tcggcgaacg gcttttggat gagccgccgc ttgacgtggc gctttttggg
    14221 ccatggatcg ggaaagaaga tgtggaatcc ggcgattgaa gcatcggcga tcatatgctt
    14281 gagtacctcg acggcatcaa agcgcatcat tttaatattg tccaaccctt cttttcctac
    14341 agcgttgagc agcttggcga ttcccgtaag aaacacttcc aagccgatat agttgtattc
    14401 gctccgcatc ttggcgatat gagcggtcgc cgccccattg ccgaacccga tttcgatcac
    14461 caccggattg tcgtttccga agagaacgga ataatcgagc atctcctctt tgaaggaaag
    14521 gccgtagcgg ggccaatatg tttcgatcgc cttcgcctcc cgctttttca aaccggcggt
    14581 tctgagcaca tagcttttta tcttccgatc gcttccgatt tcccgggtgt cgaccactct
    14641 cagctcggga aaatccttga agattgtttc attcatcaag acacctctac aaaagccgct
    14701 caaggtgctc cagcaaaaag agcgccttgt ccttcaggct caccgtttcc acctcgatat
    14761 acatgatgtt cagcacgttt cgatcaagcc gcaccttctt gttggaaagg gcgatcaggt
    14821 tcatcacttt gtcgatgttc aaatccgcca cttttccgaa ttcgatccgg acgactcctc
    14881 tgcggtcgct cagcgtggtg atggaaagct tccggcagag gatcttgatt cgggcgatgt
    14941 agagaaggtt ctccacctct tcgggaacct ggccatagcg gtcggccagc tcggttcgta
    15001 aggcatccag ctgctcgtcg ttctggatgg aggagatctt ccgatagacc tcgaacttca
    15061 cgctcggagt tttgatgtag gaatcgggaa taaagccggt ataatcaagc tccaggagga
    15121 cttggcgatc ctcctctttt tccccctcgt tctgaagggt tcgaatcgct tcatcaagga
    15181 ttcggatgta catgtccagt ccgaccgagg cgacctgtcc gctttgctgt cgtccaagca
    15241 gattgcccgt tccccgcatc tccatatctt tcagggcgat cttgaatccg ctgcccagct
    15301 cggtcagttc actgatgatg cgcagccttt tgaccgccag ctcgttgagc gagctgtccg
    15361 gcggataaaa gagataggca tacccctggc ggtcgctgcg gcctacccgg ccgcggagct
    15421 ggtagagctg ggcaagcccg tagcggtccg cccggtcgat gatgatcgtg ttcacgttgg
    15481 ggatgtcgat gccgttttcg atgatcgtgg tggaaaccag tacttggatt ccctggtgga
    15541 tgaacctgcg catctgatcc tccaaggcgg cggcttccat ctggccgtgg gcgacatcga
    15601 tgatcgcttc aggaacaagc tcgcgcaacc ggtctgcgac ccgcggaagg gactcgaccc
    15661 gattgtggag gtaaaacacc tgcccgtccc gctccagctc ccttctgatc gctttggcga
    15721 cgaggtactc gtcatacccg tcgatgaacg tctcgaccgc cctgcgctcg atcggcgggg
    15781 tcgccagcag cgacatgtcc cggatcttca aaagggacat atagagggtt cggggaatcg
    15841 gggtcgccga gagggtaagc gaatcgatat tggttcgaag cgtcttgatc cgctccttgt
    15901 cctttacgcc gaagcgctgc tcctcgtcga tgatcaaaag tccgaggttg tgatagagga
    15961 ggtctttctg gaggatccgg tgggttccga agaggacatc cacctttccc tcggcgacct
    16021 cgacgagaat ctgtttttgc cgtttcggcg ggacgatccg cgacaggagg gcacttcgca
    16081 cggggaactc tcccacccgc ttcatgaagg tccggtagtg ttgctccgca aggatcgtcg
    16141 tcggagcgag gaacgcgacc tgcttgccgt ccatcaccgc tttgaaggct gctcgaaagg
    16201 cgatctccgt ctttccataa ccgacgtccc cgcaaacaag acgatccatg acgatcggct
    16261 tttccatgtc ctccttgatc tcctcgatgc attggagctg gtccgccgtt tcatcgaagg
    16321 cgaaagaggc ctcgaactgg agctgccagt cggtgtcctt gctgaacgcg tagcccaccg
    16381 agttcatccg cttcgcatag agggtgatga gatgctttgc caggtcttcg gcgttcttcc
    16441 gggctttggc ctttttcgtc tcccacccca tgccggcaag cttgtccaac cggggaggac
    16501 taccgtcgct gccgatataa cgctggatca gattcgcctg ctcgatcgga acgtagagat
    16561 tctccctgtc cgcatagacg atcttgatga agtcccgctc ccgatcaaag gaggagaccc
    16621 gatcaatctt ctcaaagcgc ccgattccgt agtttacgtg cactacgtaa tccccttcgt
    16681 tcagatcgac gaaggaatcg aggggggatg tttgggtgtg tcgaagggtc ttgaccacct
    16741 cgcgccttcg cccgaagatc tcatgctcac agatcgccaa cacctgaagc gagggaattc
    16801 cgaagccgcc ggaaagttcc cgctcttcaa aagccagccc ttcgatcgag gaaagcatct
    16861 gctccaaacg ctgtctctgg agcgtgttgg aggcgaagat ggtcggcttc caccctcccc
    16921 tcagaagcgc cttcaaatca tctttgagga gggtgaaatt tccgaagtat gatcggggac
    16981 cgtcaaagtc aaagcgatgg ttgccgccgg accgtcgggt gatgtcgcag atgatcactg
    17041 cgggattctc ttcgacgaag agaggataat ccaagagaag atcattcggt ttggggacgt
    17101 tccgatcgat ctgaaacgcg gtacggtaga gacttttcgc ttcgctttga agactcttat
    17161 aggacgtaag caaacgttca tcaccgatga acgcaagggc atcgcgcttt tggaggtact
    17221 ccttgatcga ggcatactcg attgttccgc cttcatggag aatgttgatc gagacgcttc
    17281 ccaccgtttc gatcgtttcc tgggtgatcg ggtcgaaggc ggagatccgg ccgatcttgt
    17341 cccaatcggc gtagattcga tagggtcgcg ccccttcaaa ggggaagatg tccagcactt
    17401 caccccggac ggaaaactgt cccatcgccg aggtgaaggg cacctgcagg taggagcctt
    17461 cggccagctg gcgggcgatc aacaccgggt cgaacactcc tccccgtttc agttcgagct
    17521 ccgaccgctt cagcgacgcg ggggtaatca ggggagatac gaacgtcctg agggtcgtga
    17581 tgatcattcc atgcttcaag gaaagaattt cactgagccg attcgcctgt tcatactcga
    17641 cgttgtcgat ctccttttcc gcatacaggg tgcgtcccgt cgaaggcaga tagtgaatct
    17701 tcatatcctg cgcggtccgc ttgatcaagt ccccgctttt gggatccaac gccaaaccca
    17761 gatccaacag caaggtacgg gcgacttctt cagtgggggc aacgacccag agccgttgat
    17821 tgccaatctt ggtgatttgt ttcaaaaaga tcgccaaagg atatccttcc agcccctcga
    17881 catgcaagcg ggtttcggag ccccggtatg ccttgtaggc tgagccggat ttgatatatg
    17941 attggacaga ggtggtaatc ggggtttgca taagcgcctg ttaccgtacc acgtgaaaaa
    18001 gggttgtgca agagaatgaa aaacgagttc cttgatctgt tggctgaaga ggttgttcga
    18061 tgcggaacct atgcgaaaga gcgccaaggt tcggttctcc gttccttcaa aagcgacggg
    18121 acggtgatca ccgaggtcga tacgacgatc agcgaacgcc tcaccgcctt catcaaagcc
    18181 catcttcctc tggcgaacat cgtcagtgaa gaagcaatcg atccgtttga cgaagaagct
    18241 ccatacacgt tcgtcatcga tccgatcgac ggcaccgacg ggtacagcca aggcctgccg
    18301 acgtgggcgg ttgccttggg cattcttgat aagaaacgca acccggtcgg tgcgatgatc
    18361 agcgcccctc ggatgggtat cggagtcgat aatctgttca tccgcctgga cccggggaaa
    18421 gagctcttga taaacggcaa accgtttttc cccaatcctc ataaagatgt gatccaccaa
    18481 gtcgcaatgg cctcaagcgg ccatcgcctt tgcgacttca ccaactttca tggaaagatt
    18541 cgggtcttcg gatcggcgat cctccatctt ttggccccgg tgatctaccc cgagattcaa
    18601 ggagcgatga actcgtcggt ctatgtctgg gatttttgcg ccgcccacgc ggtactttgc
    18661 cacttcgact accgtctttt gtatgaagat gggcgacctg tcacctacac cgaccgcctc
    18721 ctgaaagaac ggaagaatac cggagagttc atctttgccg gaacgatcaa cggggtcgtc
    18781 gatctgcagc acaagatcaa agttgttccc cggtaggaat gtacccccga tcgacccagc
    18841 tgcgggccag atagatgcag ggggtatcca ataccgccac gatccatttc aaaagatacg
    18901 tcgagatgcc gatctgaagg agcacccccc ggggatagac accaagaaaa gcgattgcgg
    18961 taaagagcac cgtgtcgatc agctggctga cgacggtgga gaagttgttg cgcatccaga
    19021 gcgtctttcg ccccggtctc ttttctttcc agtatgcgaa cgcccacacg tcatggagat
    19081 tgcttgccag atatgcaatc agcgaagcga gggcgatccg cggcatcagg gagaagatcg
    19141 caagcatcga atcgtgtacc atatccgcag gtgacggagt gaagaccagc gcaagctgca
    19201 tcagcacggt catcgccacc agactgaaaa acccgattcc gaccgccttg tacgcttctt
    19261 tctttccgaa aaactcactg agaatgtcgg tggccaaaaa cgaggtcgca tacacgatat
    19321 tgcccaaggt cgcttccagg ccgaagagcg ttacgttctt cgtcacctgg atgttcgcca
    19381 cgatgacact gacgggaatc cacgcgaaca atccggtctt tccccacaac cggaacgccg
    19441 tcaggataaa gacgaagttg acgaggagca ttccgaacca aaacaattca ttcatatatg
    19501 ttgaagggta taccgcaaga agctaaaaaa aaacaaggcc ttttgcctct caaagcgtct
    19561 tctctcctta aatttcaata tcattttact tcttccattc gattactctc ggaattctta
    19621 tattcgaggc acccaccggc atcaagctga aaatcaatgg ttgaaaatga agagaaaaag
    19681 agttagactt gtctaacaaa tagttagctg aggctaacta tacttggagc tctcatcaat
    19741 ttagtgaaaa gcagtgatat gaggaggcga tcaaatgact acgcttaaca aagttaatgc
    19801 aggtcgaaaa gttgtcgttt cagacgtaaa aggcgatcag cggtttctaa gccggataac
    19861 atccgttgga ctaactatag gttgtgagct tgagatcttg cacaacgaaa aaaagctccc
    19921 tttgcttttt tacggtcgag atactgtagt cgctcttaat cgagaggaaa gcaaaaacgt
    19981 ctttgtggag gtgattagat gaatgcagca acgattaccg gacaccctac gattgctctg
    20041 ttaggccagc caaactccgg aaaatccacg ctgttcaacg gcttgaccgg cgcccgtcag
    20101 cgtgtcggca actggcccgg caaaaccgtt gagaagaagg aaggatatta tgtcagagac
    20161 ggcatacgtt atcgtgtcac cgacttgccc ggcacgtata gtctctctgc caattcggaa
    20221 gaggaaatca ttacacgcga ctacatcgct tccggcgagg cggatgtcgt ctgcattctc
    20281 gccgactctt ctcaactatc cagaagtctc ttcatgctgg ccgactatgc tggtattgag
    20341 tgccctgctg tactattgct gaatcttgtg gatgtagccg aggctcaggg gaaaacggtc
    20401 gatgcatcaa aactcgaaga taaacttgga atccccgtca tcccttttgt cgctgctgac
    20461 cgcaagcaat atggagcttt ttttgaaacc gtcgatcgag ccctgaagga gaagaagcga
    20521 atcaacatca attctttaac tgcaaagtat tacaccattg gaaacgggat gtatgagaaa
    20581 cttctcgcct tgatgccaga agaagggatt gggaactatt ctgccccttg gcttgcaaca
    20641 aagctcattg agggagacac agcgattttg gacaaggtga aaaacacctt ggaaaccgaa
    20701 gcgtatcgag atgtacagac gattctctct tctattcaaa acggcgcttt gcatacaagc
    20761 ggatgtaagt ttcaatgggt tgatgaactg ctttccgatg tgctcatcca aaaacaagaa
    20821 aaaccgcttt tttccaaatt tgacaggctg gcgactcatc ggagatgggg gaaaccgctc
    20881 gctgtgttca tcatccttct tggacttgtt gcctccttca ttcccgccac accgattatg
    20941 gtactcggca gcatgatccc cagattggga gggccaattg cagagatttt gagctcgatt
    21001 ggcgtatcgc agattctgat tgacctgatc aatcaagttg tgctgaacac cttgtatttc
    21061 gctgtggcca tgatcggctt tgtcctcggt gtcaatcttg tgttcggatt tttggaagaa
    21121 gtaggctaca tggctcggat ttcctatgtc tttgacggca ccatggccaa actggggcta
    21181 caaggaaaat cggttatgcc cctagtcgtc agcttcggct gcacgatcgg tggagccgca
    21241 ggtactcgcg tcatcgactc ctgggggcag cgtgttctga caattgcctt ggcttgggtt
    21301 gttccctgtg ccgcaacatg ggccattgta cccgtgattt cgtccctgtt cttcggagca
    21361 tgggctccgt taatcattgt cgccatcttc attgtcgccg gtctacatat gctgataacg
    21421 gccaaaattt ttggctcgaa attaattcga gaagaagatc gcgcaggatt gattatggaa
    21481 ttgccgcctt atcacaagcc aagatggaag gcattattgc gctcgatctt tgttcggacg
    21541 tgggatattt tagctagggc gcttcgggtc atctttcttg tatctttagc tttctggctt
    21601 ttgacgtata gtagcttggg caatccggat gcatcgctgc tgtatcgttt cggtcgggcg
    21661 attgagcctg taacgattat cttcggcttg acatggcaga cgttcttagc ctacttcgct
    21721 tccatgatca gcaaggaggc tgctttgggt gttctaggct cactatactt gggtgccgga
    21781 agtttattca ctgcaacgat tggatccggt ggtaaagtgg cagtcggact tgaaagtgtg
    21841 cttcttgctt cgcttacggc accggcggct ttagcgttca tctttgccgt taccttcaat
    21901 gcaccgtgcc tcatggccat tacatctact tatcaggaaa cgagatcgtt gaaatggacg
    21961 ctgcgtatcc ttggatatta tttttcgacc tctctgattc tggcttttct ggtgtatcgt
    22021 atcgccaatc tgttcatgta ggtgaaaggg gatgctcgtg gacggtgaag cccaatccgg
    22081 gcatcctttt gctcacatgc ccgtgatcgt taacatatct cgcgaacatt cggtaacgct
    22141 ttggaagctt gatgatgtga tcattttgtg tagcgatagg ggttttcgca cctccaccca
    22201 acctgtatcg atggagtgaa tgccctttct aaagacggat gatccgagag atcctttctt
    22261 ccaagtcaaa tgtcttgagt acttgatcga ggagagtgtc gcggacgg
//
LOCUS       DAOSLI010000032        19815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_2988_length_19815_cov_24.308249, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000032 DAOSLI010000000
VERSION     DAOSLI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 19815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19815
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_19815_cov_24.308249"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1437
                     /locus_tag="PLF67_05925"
     CDS             220..1437
                     /locus_tag="PLF67_05925"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOE89585.1"
                     /translation="MEKKKIILAYSGGLDTSVILKWLVNKGFDVVAFVANVGQDEDFD
                     AIRKKALATGASKVYVEDLRSQLVTDYVFPALLANTVYEGGYMLGTSLARPIIAKAQI
                     EIARKEGTTYVSHGATGKGNDQVRFEFGYYMHMSEVNIIAPWKDSEFLAQFEGRSDMI
                     AYAKEHNIPIKASIAKPYSEDENLIHISHEAGILEDASKRPDEDVFSHTLSPKEAPDE
                     ETLLEISFEDGLPTKVTNLNDDIVVSDPLEMILYLNKIGHDNAIGRADMVENRYVGIK
                     SRGVYETPGCEILWKAHRMLEGITMDKEAMHLRDSLMPKYAELIYNGYWGAPEFNMLS
                     ALFAESQKQVTGTAKVALYKGNVIFAGVKSPVSLYDEALGSMDKAGGYNPIDCKGFIR
                     INAIRLIAQSKRK"
     gene            1495..1965
                     /locus_tag="PLF67_05930"
     CDS             1495..1965
                     /locus_tag="PLF67_05930"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOE89586.1"
                     /translation="MNAKHYMHLAIERARETMNKGLGGPFGAVVVDKNGHVFVASNSV
                     LGDCDPTAHAEINAIRMACEANGTHDLTGAVLYATGHPCPMCLSACIWANIKDVYYAA
                     RAEDAEKIGFRDDRIYHYLEGGHQDKSVLTIEEIGREEALKLYEEYAEIEHTLY"
     gene            complement(1971..2282)
                     /locus_tag="PLF67_05935"
     CDS             complement(1971..2282)
                     /locus_tag="PLF67_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOE89587.1"
                     /translation="MKTDEVTLPLEEEIVDIADFFKVFGDPTRLKILFLLEQGERGVN
                     SICEELGMQQSTISQQLKLLRARRLVRYRKDGRNVLYRLNDEHIHAILALGIEHYQEL
                     Q"
     gene            2412..3707
                     /locus_tag="PLF67_05940"
     CDS             2412..3707
                     /locus_tag="PLF67_05940"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOE89588.1"
                     /translation="MDIKKDAAKLILEDKEVTLPIITDSMGDKAIDITSLRKQTGFIT
                     YDPGYANTGSAMSEICYVDGEQGILRYRGYDIEDLVEHCEFVEVAHLLIKGELPNVQE
                     RHLYSEMLNRHSLLHIDMRNFFRDYPQDSHPMSILAAMVASLSAFYPELADADPQANL
                     DLTVTRLLSKMRTIAAFTYRQMNGLDFVNPSYKFSYCENFLNMMFYTSVNAYVPPPLH
                     VRALNILLILHADHEQNCSTSAVRLVGSSDANLYASVAAGCCALWGTKHGGANQAVMQ
                     MLLKIHNEGLTVEQVVEMAKNKENGFRLAGFGHRIYKTYDPRAKIAKDLCQKVIGNTE
                     LDPLLQIALELEQVALSDPYFIERNLYPNIDFYTGIIFHAMGIPESMFTVLFALGRLP
                     GWIAHWLEWAGDPYQKIARPRQVYTGLAKRNVVPLVDRV"
     gene            complement(3730..4911)
                     /gene="icd"
                     /locus_tag="PLF67_05945"
     CDS             complement(3730..4911)
                     /gene="icd"
                     /locus_tag="PLF67_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOE89589.1"
                     /translation="MTRTICYIEGDGVGPEVTAVMLKAVDAAVGKAYQGAKTIQWKEV
                     LAGQKAFDRVGTYLPEETLEAIRKHKVAIKGPLTTPVGRGFRSLNVALRQELDLYVCL
                     RPVEYFEGTPSPVKHPELVDMVIFRENTEDIYAGIEWEAGSEGVKRVLAFLAEEMHVS
                     SIRFPETSAIGVKPVSKEGSERLIRSAIEYALAHKRRSVTLVHKGNIMKFTEGGFQKW
                     GYELAACEFGAVKEPDGYHIKREGEADLIINDCIADAFLQNILTKAEAYDVVATLNLN
                     GDYISDALAAQVGGIGIAPGANINYQSGHAVFEATHGTAPDIAGRNLVNPLSLVLSSV
                     LMLRHLGWDEAAALLNEAVRKTIRTGIVTKDFYSLIEKEGRSATLLGTKEYGERLCAL
                     I"
     gene            complement(4911..6848)
                     /locus_tag="PLF67_05950"
     CDS             complement(4911..6848)
                     /locus_tag="PLF67_05950"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOE89590.1"
                     /translation="MALTLTEKILSSHLKTGTLKRGNPIGISIDQTLTQDATGTMAYL
                     QFESMGLDRVQNELAVSYVDHNTIQVGFENADDHRYLQSVAAAKGVIFSRPGNGICHQ
                     VHLERFGVPGKTLLGSDSHTPTGGGLGMIAIGAGGLDVAVAMAGGPFHLISPKIIEIR
                     LTGKLRPWVSAKDVILAVLGRYGVKGNVNCIFEYTGEGVKTLQVPERATICNMGAEAG
                     VTTSIFPSDENTRAYLAAHDREEAYTPLSADEGAVYDETFELDLSTLVPKTAYPHSPG
                     NIADVSALVGKKVDQVLIGSCTNSSYADMKKVADILDGKHIAPNVSFGVAPGSREVIL
                     MLAEDGSLGKLVASGARILETACGFCIGNHQSPGTNEVSLRTSNRNFEGRSGTKSAQV
                     YLVSPETAALSALTGVFTDPLGDHGIEYPHVVNPEKFIVDDSMFIFPPEDGSAVEIIR
                     GPNIGDPPANVPLSDSLDGYVTIKVGDKITTDHIMPAGARLKYRSNIPVYSKYVFEPI
                     DEFFPQRCMENQDRGKDNFIVAGASYGQGSSREHAAICPMYLGVKAVIAISIERIHQN
                     NLCNFGIIPLTFIDEADYEKLDQNDEIVIENIRRQIAGETVILKNRTKSLEIPLRCDV
                     TGEQRKMLIKGGLLNILKEIG"
     gene            complement(6869..8053)
                     /locus_tag="PLF67_05955"
     CDS             complement(6869..8053)
                     /locus_tag="PLF67_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE89591.1"
                     /translation="MKSNSIQFHTINLASFFSQLAIAMINLTLVYHLRYHYNLPSALI
                     GLAAAMGPTSYLIACLVKGKLSSNLRPRHAIMLSLAGMSGSILLLLSVHHLAITFSCL
                     FLYGMWMSFLWPHVEEWFSRGKEGEELSKAVNGFNVSWSLGVALSSYLAGLLIEQSTI
                     LAFLVSIGFFIAVFIGIWTVSTLVPAIRMVPSETTHIETSGLTDRSTPLRFYAWIGIV
                     VLYSGMSIILTIFPLYAHEELLLRESRVGLLLLIRGVTSCVSFHLLGKFSFWHFRKSL
                     IFLVQALFALLCFSALRFSSLVFYGIFFFLFGVLFSFAYTQSIFHGSSGAIRRSTRMI
                     IHEVLLTIGTILGSVGGGWFYQKFGFANVLLAVGIFGAVAVGGELLFSIYNQRRFRYN
                     GA"
     gene            8107..8559
                     /locus_tag="PLF67_05960"
     CDS             8107..8559
                     /locus_tag="PLF67_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOE89592.1"
                     /translation="MNNNLLANSAFISAMLAFLIAQFLKPFVNVLFERRFVWHLLFST
                     GGMPSSHTAGVVALVTSIGLTYGVGTVYFAISATFASITIHDAMGIRRAAGKQAEVIN
                     EWSRILSDIHNEGQFTPENLKTMLGHSFSQILGGTILGLVIGLLVTNY"
     gene            8657..9412
                     /locus_tag="PLF67_05965"
     CDS             8657..9412
                     /locus_tag="PLF67_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE89593.1"
                     /translation="MKKWIALFILVVLVAGATLFAGGKKESAEGYVFASDATWPPLEF
                     IDENGNLTGFEVELIPLIASKVGATMTVKNIPWDTIFAGLANGAYDAIASGVTVTEDR
                     KATMDFSTTILEAGQVVIIRAADQGKYNGIDDLNGKAIGVQIGTTGDFALEEYPVVTR
                     AYDEIGLAIEDLLNKNVEAAVCDSLIASDFVLANPNYKGKLMVVGEAFTQEDIAIAVQ
                     KGNTKLLGLINKGLEIAMEDGSFDALKAKWNLL"
     gene            9521..10375
                     /locus_tag="PLF67_05970"
     CDS             9521..10375
                     /locus_tag="PLF67_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOE89594.1"
                     /translation="MENQELDEVKLEMVRKKVLSVDPHAAKKKIPTITMTDGVLIPRK
                     DSGHLFNSWRITFISALSLLAFLVVFKPDPYRSIFLVTIKGAPVTFQATIFAIIGAIV
                     IGTITGLGSVSKKHWVNIVSGIYVETIRGIPLLVQLIFIYYAMGRFFKIEGMLAAVTA
                     LAICFGAYMGEIVRAGIQAIPKGQMEAAIALGLSRTQAFRYVILPQTVKVILPAIGNE
                     FISMLKDSSLVSTIALSDILRKGREYISRTFLSLETMLIVALIYLVITLMLSRLVGIM
                     EERLHRHG"
     gene            10368..11111
                     /locus_tag="PLF67_05975"
     CDS             10368..11111
                     /locus_tag="PLF67_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOE89595.1"
                     /translation="MARIVLENLCKDYHVADHKTVHAVVDASLTVDDGQVVVIIGPSG
                     SGKSTLLRSINRLEEPTSGRIFIDDDEVTAKDVDIRSIREEVGMVFQSFNLFPHLTAL
                     ENITLAPMKVRKLSKKQAHDEAMELLKLVGLEEKANVHPPKLSGGQQQRVAIARALAM
                     NPKVMLFDEPTSALDPEMIKEVLDVMLKLAKSGMTMLLVTHEMGFAKAAADLIVFMDE
                     GSIVEIAPPDELFSKPKSDRTKHFLEHIL"
     gene            11187..11852
                     /locus_tag="PLF67_05980"
     CDS             11187..11852
                     /locus_tag="PLF67_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOE89596.1"
                     /translation="MKTESWLQKHITLHDYDDPKAERANALTHGFGAILAVLALGMVI
                     GRFSFYSSGSLARGMVLFCLTMILLYSSSTLYHLAPKSDAKRILRVFDHSSIYVLIAG
                     TYTPLMLGIDSDTARMVLFLVWTIAVAGIILTFLFWGKFKVLRVLLYVVMGWLVVFFW
                     GDIIPFLPQGLFPYVLAGGITYTVGVAFYASKRLPCHHAVWHLFCIGGSVPFFIGFFV
                     KLR"
     gene            complement(11824..12582)
                     /locus_tag="PLF67_05985"
     CDS             complement(11824..12582)
                     /locus_tag="PLF67_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE89597.1"
                     /translation="MNKQTRNLLIAIIAFLLVIILAAVLYDKLVDKAAPTVSYAPVLR
                     ELPQSGSAMDAKASVDEAPTAAKEVAKEERLPQSESAMSVAAPVDEESPDVTEEAEEE
                     ESDELKMPNIPLFRLDGTQTDFYEVADGKPVVLNYFASWCPPCQAEMPDFVKAYNAHK
                     DEVTFIFLDSLDGERESLATLKTFIRKQKMDESSVFYDEGIFAYIFQTSSLPTTVFFY
                     GDGAIAGGQLGMVNEQYLQYAIEEITSEASRKSR"
     gene            complement(12592..13263)
                     /locus_tag="PLF67_05990"
     CDS             complement(12592..13263)
                     /locus_tag="PLF67_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOE89598.1"
                     /translation="MAYLTAFLEGIISFISPCILPILPLYFSYLAGGITDREERKGVL
                     LTNSLAFVLGFTILFVALGAASTSIGRFLSGHLELLNRIGGVLVILFAVNNLGFVLIP
                     ALNKNRQFQFKGLHNMTIGKSILFGIVFAFGWTPCVGPLLGSALMMAANATMMYKGML
                     TLLFYALGLGIPFLLSALLLDQLEGVFNVMKKHSNLITIISGVFLLIFGVALLLGFNP
                     AALFL"
     gene            13328..14101
                     /locus_tag="PLF67_05995"
     CDS             13328..14101
                     /locus_tag="PLF67_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HOE89599.1"
                     /translation="MVKNTPKVPTFDEYYRVFYQDRWESLKTALLEERKPVAYTEGLL
                     QPYYLDEASIAAARALGVSEGDRVLDLCAAPGGKTLVLATALKGSGSLTANDRSSARR
                     ARLRDVITNHLPEQDRANITITGHDAAKWSLYEQDAYDRILLDAPCSSERHVLSDEKA
                     LKDWSPSRPKRLAVNQFAMLASALEAVVPGGLILYSTCSINPLENEEVIAKLEKRRAG
                     RFEELHLDRGEKRSFGSIILPDVSDGKGPLYMCLIRRLT"
     gene            14098..15216
                     /locus_tag="PLF67_06000"
     CDS             14098..15216
                     /locus_tag="PLF67_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOE89600.1"
                     /translation="MSCVSIVRCESYEPALVDKAVREACASAGMPDVNGKTVLLKPNI
                     LSDSKEELGITTHSQVVRSVIRLLKEQGAKRILVGDSPGLHTPNFRPRASGIWKVIRE
                     EEVEWADFTDRPTTYPIPYARGKKLPLPSLLEEVDLLFSLPKMKTHQLMYATGAIKNL
                     FGLVPNLHKSPCHLSFPTREQFASLMVGIATVAKASFALMDGIIAMEGPGPANGSSLP
                     MGLILASTDLVALDYAQAEIMGYTPTDVPIVAEGLKRGLGKAPSEYPNLQTHDLIKTD
                     YKRIEVQKKTRFFHALILPFFAGPFVRWRVKRQRRAPFFLKEECILCKKCIQICPVDA
                     LRIEEKHIVIDEKRCIRCYCCHEVCPASAILVDEEIPS"
     gene            15213..>15505
                     /locus_tag="PLF67_06005"
     CDS             15213..>15505
                     /locus_tag="PLF67_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOE89601.1"
                     /translation="MTSIHCLITIALAVLPISELRGAIPYAYFNGAPLFLAALIGVLA
                     NLLVAPIVYLFLGSLHRVFYRIWGWYRSFFDRFVERARKKVEHHFSKWGYIGLA"
     assembly_gap    15506..15605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15606..16817)
                     /locus_tag="PLF67_06010"
     CDS             complement(<15606..16817)
                     /locus_tag="PLF67_06010"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOE89602.1"
                     /translation="MEGRRYLKRIADRLLQERLRSSGAVLIEGPKWCGKTATATRASK
                     SQLFMQDPDMTVSYLSAADTKPSLLLKGETPRLLDEWQSAPILWDAVRFMVDQRGKPG
                     QFILTGSAVPKDDAVQHTGTGRISRLMMRPMSLFESLESNGSVSLKSLFDGHINVEAV
                     SDLSIEGIASALVRGGWPASIGDDESIALRHAVDYVEAIINADISRVDGVEKNPARVR
                     ALMRSYARNIATTATMRTIRDDIALGDADASISEKTISQYLVALDRMFVTEDLPAWNP
                     ALRSKTAIRSSVKRHFVDPSIATAVMRLTPFRLLEHFEYFGFLFESLCTRDLRIYADA
                     IDGQVFHYRDASGLESDTVVCLNDGRWAPIEVKLGTKAIEEAAEHLLELKAKVDTGKM
                     NEPSFLMILTGG"
     assembly_gap    16908..17007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17154..18008)
                     /locus_tag="PLF67_06015"
     CDS             complement(17154..18008)
                     /locus_tag="PLF67_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89603.1"
                     /translation="MAVAGNIANVQGTSAQPYLVDIFLINTNADINSKGGIHATANSG
                     NPGSGFFLNTPYAFKNPFNPCFGLATANQPNSNMYDYMNGTTPKTGQNDQYKGAYVPV
                     ASPSGTNTGHATTPIIPGGAMSGGEPGQGGGNGGIVYGDEPISDPVIHTLFSLESEDV
                     PIILSQFTGTQVKQINTATLEVVVENGTGGEKISVEVLFTDDSPVGWNGFHLLHETPG
                     PTIPYNLYWGDTTTRITKDSPIQWNNLVAGEHTKTIYMGRIDETFVNQAAEGRYKSTI
                     TVTLTNSN"
     gene            complement(18512..19672)
                     /locus_tag="PLF67_06020"
     CDS             complement(18512..19672)
                     /locus_tag="PLF67_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89604.1"
                     /translation="MGSVRYTVVLLMLLLVPALLFGAITAVYTPEPYLVLQAKPGPFT
                     SDTTFGAKLGTLIVTTTDGDKIYAPTWGSTDEVEYSAILRGPMRSYPGGPFEENATHT
                     FHIMSVAYPYGYPGTPVIRVVQSPHSPIVSWFANTVDANPFRVELYLMSTNSTYQHTT
                     TYWRPASYFQLNAVYTLPITFNPVFSFVSANDYWMNVGWMMNNGEPNPSHGSYVSVNG
                     VAGADSTPIVQSSAYTDPDNPGQPGMTYGDPPQMVSYSVFLTNQSTEFNLADAIGSKR
                     KDVNTMSITVSNGTAGANYTQKVIFTDLTPVSTSAEFRMLPEESGPNPIPFKLYFDST
                     EVVKGEAITWNNLQNGSSNTKTIRIGGINQNTIDSLASGTYSDTISVEIQNP"
BASE COUNT         4486 a         5305 c         5244 g         4580 t
ORIGIN      
        1 agttcagggt gttaaaacaa gggaaagcga atgatttgtt ctacgcatcg gtggaacaat
       61 gaacaatcat aaaaacagat aaaatgtata tctattcatc gatatgctga ttatctcaca
      121 ataattggca atatatcggc atataaaagt ataaatatac atagaccgct tgtctttctt
      181 gacggatatt tgtacactgc gcccataagg aacgaagata tggaaaagaa aaaaatcatt
      241 ttggcgtaca gtggcggttt ggatacctcc gtcatcttga aatggctggt gaacaaaggg
      301 tttgacgtgg tcgctttcgt ggcaaacgtc ggacaggacg aggattttga cgccatcagg
      361 aaaaaagcat tggccaccgg tgcttccaag gtgtatgtcg aggatcttcg aagtcaattg
      421 gtgaccgact atgtcttccc cgctctgttg gcgaacaccg tctacgaagg cgggtatatg
      481 ctcggcacct ccctcgcccg gccgatcatc gccaaagccc agattgaaat tgcccggaag
      541 gaaggaacca cctacgtctc ccacggcgct acgggaaaag ggaatgacca agtccggttc
      601 gagttcggtt attatatgca catgagcgag gtgaacatca ttgcgccgtg gaaggacagc
      661 gagttcctcg cccaattcga gggtcggagc gatatgatcg cgtatgccaa agagcataat
      721 atcccgatca aggcctcgat cgccaagccg tacagcgagg acgagaacct gatccacatc
      781 agccacgagg cgggaattct ggaggatgca tccaaacggc ccgatgagga tgtcttctcc
      841 cacaccctca gccccaaaga agctcccgat gaggaaacac ttttggagat ctcctttgaa
      901 gacgggttgc cgacgaaggt gacgaacctg aacgacgaca tcgtcgtcag cgatcccttg
      961 gagatgatcc tctacttgaa caagatcggc cacgacaacg cgatcggacg ggccgacatg
     1021 gttgaaaacc gctacgtcgg catcaagagc cggggggtgt atgaaacccc cgggtgtgaa
     1081 atcctttgga aggcgcaccg gatgcttgag ggcattacga tggacaagga ggcgatgcac
     1141 cttcgcgact ccctcatgcc caaatacgcc gagctgatct acaacggcta ctggggagcc
     1201 cccgagttca atatgctgag cgccctcttt gcggaaagcc agaagcaggt gaccggaacg
     1261 gcgaaggtag ccctgtacaa aggcaacgtc atcttcgccg gggtgaagag tcccgtctcc
     1321 ctgtacgacg aggcgcttgg ctcgatggac aaagccggcg gatacaaccc gatcgattgc
     1381 aaggggttca tcagaatcaa tgcgattcgg ctgatcgccc aatcgaagcg gaaataaaac
     1441 gtttttgcgc tcccatccga ttcgtggtat tatggaattg ccgggagcgc agtcatgaac
     1501 gcaaaacact acatgcacct cgcgattgag cgagcacggg agacgatgaa taaaggactc
     1561 ggcggaccgt tcggggcggt tgtagtagat aagaacggtc acgtttttgt cgcctcaaat
     1621 tccgttttag gcgactgtga cccgaccgcc catgcagaga tcaatgcgat ccgaatggcg
     1681 tgcgaggcca acggcaccca cgatctgacc ggtgcggtcc tctatgcaac cggacatccg
     1741 tgcccgatgt gcctcagcgc ctgcatctgg gccaatatca aggatgtcta ttacgccgct
     1801 cgggctgaag atgccgaaaa aatcgggttt cgggatgacc gtatctacca ctacctcgag
     1861 ggagggcacc aggacaagtc ggtcctcacg atcgaagaga tcggccggga ggaagcccta
     1921 aagctttatg aggaatacgc tgaaatagaa cacaccctgt attgagaacg ctattggagc
     1981 tcctgataat gctcgattcc gagggcaaga atcgcatgga tgtgctcatc attcaggcgg
     2041 tagaggacat tgcggccatc cttccggtag cgcaccaggc gccgggctct gaggagtttc
     2101 agttgttgac tgatcgtgga ttgctgcatc cccagctctt cgcagattga gttcactccc
     2161 cgctctccct gctccaataa aaagaggatc ttcaagcgag tgggatcgcc gaataccttg
     2221 aaaaaatcgg caatatcgac gatttcttcc tccaggggca atgtcacctc atcagtcttc
     2281 atctttccgt cctttcgttg attatagccg ctgcaggtgc aaatgaaaag aaaatctcct
     2341 gtaagcccac cctattctca aaaaaagaaa agtactgtag tgtttgctat cccctttaag
     2401 gagatggatt gatggacatt aaaaaagacg cagccaaact tattcttgaa gataaagagg
     2461 tcacccttcc tatcatcacc gactcgatgg gcgataaggc gatcgatatc acttcactgc
     2521 gaaagcaaac cggcttcatc acctatgacc cgggatacgc caatacgggc tctgcaatga
     2581 gcgagatttg ttatgtggac ggtgaacagg gaattcttcg ctatcgggga tacgatatcg
     2641 aagacctcgt tgaacactgc gagttcgtcg aagtcgccca ccttttgatc aaaggcgagc
     2701 tccccaatgt gcaagagcgc catctctatt cggaaatgct caaccgccac tccctgctcc
     2761 acatcgatat gcggaacttc ttccgcgact atcctcagga ctcgcacccg atgtcgattc
     2821 tggcggcgat ggtggcttcg ctttcggcgt tctatcccga gctcgccgat gccgacccgc
     2881 aggcgaacct cgacctcacc gtgacgcgcc tcctttcaaa gatgcggacg atcgctgcgt
     2941 tcacctaccg gcagatgaac ggtcttgatt tcgtaaaccc ctcctataag ttctcctact
     3001 gtgaaaactt cctgaacatg atgttttaca ccagcgtaaa cgcctacgtt cccccaccgc
     3061 tccatgtccg ggcattgaac atccttctga ttcttcacgc cgaccatgag cagaactgct
     3121 caacgagcgc cgtgcggctc gtcggttcca gcgatgcaaa cctctacgct tcggtcgccg
     3181 ccggctgctg cgccctgtgg ggaacgaagc atggcggagc gaatcaggcg gtgatgcaga
     3241 tgctgctgaa gattcacaat gaaggcttga cggtcgaaca agtcgtcgaa atggccaaaa
     3301 acaaggaaaa cggattccgc cttgccggct tcggccatcg gatctacaag acctacgatc
     3361 cccgggccaa gatcgccaaa gatctctgtc agaaggtaat cggaaacacg gagctggatc
     3421 ccttgctgca gattgcactg gagctggagc aggtagcctt gagcgacccg tattttatcg
     3481 aacggaacct gtatcccaac atcgatttct atacgggaat catcttccat gcgatgggaa
     3541 tccccgagtc gatgttcacc gtcctctttg ccttgggccg ccttccgggc tggatcgccc
     3601 actggcttga atgggccggc gacccctacc agaagatcgc ccgcccccgc caagtgtaca
     3661 ccggtctggc gaaacgaaac gtcgttcctt tggtcgatcg ggtttgagct tgaaggtttc
     3721 ttctttcttc tagatcagcg cgcagaggcg ctctccgtac tcctttgttc cgagcagggt
     3781 agcgcttctt ccctcttttt cgatcaggga ataaaagtcc ttcgtcacga tcccggttct
     3841 gatcgtcttt ctgaccgctt cgttgagaag ggccgccgct tcatcccacc ccagatgtcg
     3901 aagcatcagg acgcttgaaa ggacgagcga aagcggattg acaaggttcc tgccggcgat
     3961 gtccggggcg gttccatggg ttgcctcgaa gaccgcgtgg ccgctttgat agttgatgtt
     4021 cgccccggga gcgatcccga ttccccccac ctgggcggca agggcgtcgg aaatgtaatc
     4081 gccgttgaga ttcagggtgg cgaccacgtc gtacgcttcc gctttggtga ggatgttctg
     4141 caggaacgcg tcggcgatgc agtcgttgat gatgaggtcc gcttcccctt cccgtttgat
     4201 atggtaaccg tccggctcct tgaccgctcc aaactcgcaa gcagcgagtt catatcccca
     4261 tttttgaaat cccccttcgg tgaacttcat gatgttgcct ttgtgaacca aggtaacgct
     4321 tcgtcttttg tgggcaaggg cgtactcgat cgcgcttcgg atcaaccgct cgcttccctc
     4381 cttcgagacc ggcttgaccc cgatcgcgct ggtttcggga aagcggatcg acgagacatg
     4441 catctcttca gcgaggaagg ccaaaactct tttgacccct tcgcttcccg cctcccattc
     4501 gattccggcg tagatatcct cggtgttttc ccgaaagatg accatatcca ccagctcggg
     4561 atgcttcacc ggtgaaggag tgccttcaaa gtactccacc gggcgcaaac acacatagag
     4621 atccagctct tgtcgaaggg cgacgttcag cgagcggaac cccctgccga cgggggtggt
     4681 caagggacct ttgatcgcaa ccttgtgctt cctgatcgct tcaagggtct cctcgggcag
     4741 atacgtcccc acccggtcga acgctttttg tccggcgagc acttctttcc actgaatcgt
     4801 tttggcgcct tggtatgctt tgccgacggc ggcatccacc gctttcagca tcacggccgt
     4861 gacttccggc cccactccgt cgccttcaat gtagcaaatc gtccgcgtca tcatccaatc
     4921 tccttgagaa tgttcaacag tccgcccttg atcagcattt tccgctgctc cccggtaaca
     4981 tcacagcgca aaggaatctc caaactcttg gtcctgttct tcaggatgac ggtctctccg
     5041 gcgatctgcc ggcggatgtt ctcgatgacg atctcatcgt tttgatcgag cttttcataa
     5101 tcggcttcat cgatgaacgt gagcggaatg attccgaagt tgcagaggtt gttctgatgg
     5161 attcgctcga tcgaaatagc gatcaccgct ttgacaccga ggtacatcgg acagatcgcc
     5221 gcatgttctc tggaagaccc ctgtccgtag gaagctccgg cgacgatgaa attgtccttg
     5281 ccccgatcct gattttccat gcagcgctgg gggaagaact cgtcgatcgg ctcgaagacg
     5341 tacttggagt agacgggaat gttggagcgg tatttgaggc gggcgcctgc cggcatgatg
     5401 tggtcggtgg tgatcttgtc ccctaccttg atcgtcacgt acccgtcaag ggaatcgctt
     5461 aagggaacgt tcgccggcgg atcgccgata ttgggtcccc ggatgatttc cacggcgctt
     5521 ccgtcttccg gggggaagat gaacatcgaa tcatcgacga tgaacttctc gggattcacg
     5581 acatgaggat actcgatccc atgatccccc aaggggtcgg tgaaaacgcc ggtcaatgca
     5641 ctcagggctg cggtctcggg actgaccaga tagacctggg cgctcttggt cccgctgcgg
     5701 ccctcaaagt tccggttgct ggtcctgagg ctcacttcgt tcgttccggg gctttggtgg
     5761 ttgccgatgc agaacccgca cgccgtttca agaatccgcg ccccggaagc gaccagcttt
     5821 ccgagcgaac cgtcctcggc aagcatgaga atgacctcgc ggctgcccgg agcgactccg
     5881 aaggaaacgt tcggggcgat atgctttccg tcaaggatgt cggctacttt cttcatatcc
     5941 gcatatgagg agttggtgca tgatccgata agcacctgat cgactttctt gccgacgagc
     6001 gctgatacat cggcgatatt gccgggcgaa tgggggtacg ccgtcttggg aaccagcgtg
     6061 gaaaggtcga gttcaaacgt ttcgtcatag accgccccct catcggcgga aagaggtgta
     6121 tacgcctcct ctcgatcatg ggctgcaagg taggcgcggg tgttttcatc gctggggaag
     6181 atgctggtgg tgacccccgc ctccgctccc atgttgcaga tcgtcgcccg ttcgggaacc
     6241 tggagcgttt tcaccccttc tcccgtatac tcgaagatgc agttcacgtt ccccttcacc
     6301 ccgtaccgtc ccaggaccgc caagatcaca tctttggcgc tgacccatgg gcggagtttc
     6361 ccggtcaagc ggatttcaat gatcttggga ctgatcaggt ggaaggggcc gccggccatc
     6421 gcgacggcaa catccagtcc gccggcgccg atcgcgatca ttccgagtcc gccgccggtc
     6481 ggggtatggc tgtcgctgcc caagagcgtc ttgccgggga caccgaagcg ctcaaggtgg
     6541 acttggtggc agattccgtt tcccggacgg gagaagatca ccccttttgc cgccgccacg
     6601 gactgcaagt agcggtggtc gtcggcgttt tcaaagccta cctgaatggt attatgatcc
     6661 acatagctga ctgccagttc attttgaacc cggtccaagc ccatcgattc aaactgcagg
     6721 tatgccatcg taccggtggc atcctgggta agggtctggt cgatcgatat tccgatagga
     6781 tttccccgct tcaacgttcc cgtcttgagg tgagagctga gaattttttc cgtcagcgtg
     6841 agggccatag catcctcctt acgaatgatt acgctccatt gtagcgaaag cgcctttggt
     6901 tgtatatgct gaaaaggagt tcccctccaa cggcgaccgc accgaaaatt ccgacggcaa
     6961 gcagcacgtt ggcaaacccg aacttctggt agaaccaccc gcccccgacc gaaccgagaa
     7021 tcgttccgat cgtgagcagc acctcgtgga tgatcatccg cgtggagcgt ctgattgcac
     7081 cgctggaacc atggaagatc gattgggtat aggcgaagga aaagagcacc ccgaagagaa
     7141 aaaagaagat cccgtaaaag acgagtgatg aaaaccggag agcgctgaaa cacagaagag
     7201 cgaagagcgc ttgaacgagg aagatcaggg atttcctgaa atgccagaag gagaatttgc
     7261 cgaggagatg gaaactgacg caggaggtaa cacctcgaat caacagaagc aatccgacgc
     7321 gggattccct cagcagcagc tcttcatggg catacaaggg gaagatcgta aggatgatgc
     7381 tcattccgct gtagagcacg acgattccga tccacgcata aaacctcagc ggcgtcgatc
     7441 gatcggtcaa tccgcttgtt tcaatatggg tcgtttcgct tggaaccatc cgaattgcgg
     7501 gaaccaaggt gcttaccgtc caaatgccga tgaacaccgc gatgaagaag ccgatggaaa
     7561 caaggaaggc gaggatcgtc gattgttcaa tcaacaaacc ggcaaggtat gatgagaggg
     7621 cgactcccaa cgaccaggag acattgaacc cgttgaccgc cttgctgagc tcctccccct
     7681 ctttgccccg gctgaaccac tcctccacat gaggccaaag aaaactcatc cacatcccat
     7741 agaggaagag acacgaaaac gtgatcgcaa gatgatgtac actcaagagc agcaggatcg
     7801 aaccgctcat ccccgccaga gagagcatga tcgcatgacg aggcctcagg tttgaggaga
     7861 gctttccttt gaccaggcag gcaatgagat acgaggtcgg ccccatggcc gcggcaagcc
     7921 cgatcagcgc ggaagggagg ttgtagtgat atcgaaggtg atagaccagc gtcagattga
     7981 tcatcgcaat cgcaagctgg ctgaaaaaag aagcaagatt tatcgtatga aactgaatcg
     8041 aattactttt cattcacacg ctcctccatg attgcccaag ttaggtaaaa cctatacatt
     8101 tatgctatga ataacaacct cctggcaaac agtgctttta tctctgccat gcttgctttt
     8161 cttattgctc aatttttgaa gccgtttgta aatgtactct ttgaacgaag gtttgtctgg
     8221 catctgctct tttccaccgg cggaatgcct tccagccata ccgccggtgt ggtcgccttg
     8281 gtcacttcca tcggattgac ctacggagtc ggaaccgtat actttgcgat cagcgcgacc
     8341 tttgcttcaa tcaccatcca cgatgcgatg ggcatccgcc gggcggcggg caagcaggcc
     8401 gaggtgatca acgaatggag caggatttta agcgacatcc ataatgaagg ccagttcacc
     8461 ccggaaaacc tcaaaacgat gctcggccac tccttcagcc agattcttgg cgggacgatc
     8521 ttggggctcg tcatcggtct cctagtaacg aattattaaa ctctcctgca tacttataca
     8581 tttattttga aattacttga cattatttgc atatctatcc atgatatcca gcatatatat
     8641 gcaaggagat tcaagtatga agaaatggat agcgctcttt atattggttg tgctggttgc
     8701 cggtgcaacc ctgttcgccg gtggtaaaaa agagtccgcc gaagggtatg tgtttgccag
     8761 tgacgccact tggcctccgc tggagttcat tgatgaaaac ggcaatctca ccggctttga
     8821 agtcgagctg attcccctca tcgccagcaa agtcggtgca acgatgaccg tgaaaaacat
     8881 cccttgggat acgatcttcg ccggactcgc caacggagca tatgatgcga tcgccagcgg
     8941 ggtcaccgtg accgaagatc ggaaagcgac gatggatttc tccacgacga ttctggaagc
     9001 gggacaagtg gtcatcatcc gcgccgccga ccaaggaaag tataacggaa ttgatgatct
     9061 gaacggcaag gcgatcgggg tgcagatcgg aaccaccggg gatttcgccc ttgaggagta
     9121 ccccgtcgtc acccgggcat acgatgaaat cggtttggcg atcgaagacc tgctcaacaa
     9181 aaatgtggaa gctgcggttt gtgattccct catcgccagt gatttcgtct tggccaatcc
     9241 caactacaag ggcaaactga tggttgtcgg tgaagcgttc acccaggaag atatcgcgat
     9301 cgccgtgcag aaaggcaaca cgaagcttct gggtctgatc aacaaaggcc ttgaaatcgc
     9361 gatggaggac ggttccttcg atgctctgaa agcaaagtgg aacctgttgt aatcaacgat
     9421 gaaacgatct tggggggagc acggctcccc ttttgtttgt ctgaataaat atacggtgaa
     9481 ccttgactta atccgcataa ttatgtaaga agaatgtccg atggaaaatc aggaattaga
     9541 cgaagtaaaa ctcgaaatgg tgcgtaagaa ggttctttcg gttgaccctc acgcggccaa
     9601 gaaaaagatt cccacgatca cgatgaccga cggagttctg attccccgga aggacagcgg
     9661 acatctgttc aactcgtggc ggatcacctt catcagcgcc ttgagtctgt tggcttttct
     9721 ggtggttttc aaacccgacc cctaccgttc catcttcctg gtcacgatca aaggagcgcc
     9781 cgttaccttc caggcgacga ttttcgcaat catcggagcg atcgtcattg gaacgattac
     9841 cggcttgggc tcggtaagca agaagcattg ggtgaatatt gtcagtggaa tctatgtcga
     9901 gacgatccgg gggatccccc tcctcgtcca attgatcttc atctattatg cgatgggccg
     9961 gttcttcaag atcgaaggga tgcttgccgc cgtcaccgcg ctggcgatct gtttcggtgc
    10021 gtatatggga gaaatagtcc gggccggtat ccaagcaatt cccaaagggc agatggaagc
    10081 ggcgatcgcc ctcggcctga gccgcacgca ggcgttccgg tatgtgatcc tgccgcaaac
    10141 ggtgaaggtg atcctgcctg cgatcggaaa tgagttcatt tcgatgctga aggactcttc
    10201 cctcgtttca acgatcgccc tcagcgacat ccttcggaaa ggccgggagt acatctcccg
    10261 aacgttcctt tcgttggaga cgatgctaat cgtcgccttg atctatctgg tgatcaccct
    10321 gatgctctcg cgcctggtgg gaattatgga ggagcgactg caccgccatg gctagaatcg
    10381 tattggaaaa cctctgcaaa gattaccacg ttgccgacca taagacggtc catgcggtgg
    10441 ttgacgcttc gctcaccgtc gatgatggcc aggtcgtcgt catcatcggc ccgtcgggaa
    10501 gcggaaaatc tacgttgctg cgctcgatca acagacttga ggagccgacg agcgggagaa
    10561 tcttcatcga cgacgatgag gtgaccgcca aagatgtgga tattcgttcg attcgggaag
    10621 aagtgggaat ggtcttccaa tccttcaacc tctttcccca tcttaccgcc ttggaaaaca
    10681 tcaccctcgc tccgatgaaa gtccgcaagc tctccaagaa acaggcccat gacgaagcga
    10741 tggagctgct caagcttgtt gggttggagg aaaaggccaa tgtacatccg ccgaagctct
    10801 ccggcggcca gcagcagcgg gtggcgatcg ctcgggctct ggcgatgaac cccaaggtga
    10861 tgctctttga cgagccgacc agcgcccttg acccggagat gatcaaggaa gttctggacg
    10921 tgatgctcaa acttgccaaa agcgggatga cgatgctcct ggtgacccac gagatggggt
    10981 tcgccaaagc ggcggcggat cttatcgtgt tcatggatga aggatcgatc gttgaaatcg
    11041 cccctccgga tgagctgttc tccaagccca aaagcgatcg gacaaagcat tttctggaac
    11101 atatcctcta gtagaactct actagcataa aagaagcggt ccaaaacctt gctcaagaag
    11161 gaccgctttt tgtagtgtag ggtgatatga aaacggaatc ctggctgcaa aagcacatca
    11221 ccttgcacga ctacgatgac ccgaaagcgg aacgggcgaa cgcgctgacc cacggcttcg
    11281 gagcgatcct cgccgtgctg gcccttggaa tggtcatcgg acggttctcc ttctactcat
    11341 ccggttccct cgcccgggga atggtcctct tttgcctgac gatgatcctt ctctacagtt
    11401 catcaaccct ttatcacctc gcccccaaaa gtgatgccaa acggatcttg cgagtcttcg
    11461 accactcgag catctacgtt ctgattgccg gaacctacac gccgttgatg ctcggaatcg
    11521 actccgatac ggcaaggatg gtcctctttc tggtatggac gatcgccgtt gccgggatca
    11581 tcctgacctt tctcttctgg ggaaaattca aagtgcttcg cgttcttttg tacgtcgtga
    11641 tgggttggct ggtcgtcttc ttctggggtg acatcatccc gtttctgccg caaggccttt
    11701 ttccctacgt tctggccggg ggaatcacct acacggtggg ggtggcgttt tatgcaagca
    11761 aacgcctccc ctgccatcat gcggtctggc acctcttctg catcggagga agcgtgccgt
    11821 tctttatcgg ctttttcgtg aagcttcgct agtgatctct tcaatggcgt attgaagata
    11881 ctgctcattc accatcccca attgacctcc ggcgatcgct ccgtcaccgt agaagaagac
    11941 agtcgtcggc aagctggagg tctggaagat gtaggcgaag attccttcat cgtagaacac
    12001 cgagctctca tccatcttct gcttcctgat gaaggttttc agcgtggcga ggctctcccg
    12061 ctctccgtcc agcgaatcga ggaagatgaa cgtcacttca tccttgtggg cgttgtaggc
    12121 tttgacgaag tcgggcatct ccgcctgaca gggcgggcac catgaggcga aatagttgag
    12181 caccaccggc ttgccgtcgg ccacctcgta gaagtcggtc tgggtgccgt caagacggaa
    12241 gagggggatg ttgggcatct tcagctcgtc cgattcttct tcctccgcct cttcggtgac
    12301 atccggactt tcttcatcaa ccggtgccgc cacactcatt gcgctttcac tctgcggaag
    12361 cctctcttct ttcgctactt ccttggcagc ggtgggagct tcatcaaccg atgccttcgc
    12421 gtccatcgca cttccacttt gtggaagctc ccgcaacacc ggggcatagg agacggtcgg
    12481 agcggctttg tccacaagtt tgtcatagag gacggcggcg aggatgatga ccagaagaaa
    12541 tgcgatgatc gctatcaata aattgcgagt ctgtttattc atggaacgct cctataaaaa
    12601 gagtgcggcg ggattgaacc ccaagagcaa ggctacaccg aagatcagga ggaatacccc
    12661 gctgatgatc gtgatgaggt tggagtgctt tttcatgacg ttgaacaccc cctccaattg
    12721 gtcgagcagc aacgctgaaa ggagaaaggg aatacccaac cccagagcgt agaagaggag
    12781 cgtcagcatc cccttgtaca tcatggtggc gttcgccgcc atcatcagcg ccgagcccag
    12841 aagcggcccc acgcaggggg tccagccgaa ggcaaacacg atgccgaaga ggatgctctt
    12901 gccgatcgtc atgttgtgga gtcccttgaa ttgaaactgc cgattcttgt tcaacgcggg
    12961 gatcaggaca aagccgaggt tgttgaccgc aaagaggatg acgaggacgc ccccgatccg
    13021 gttgaggagc tccagatggc cgcttaagaa ccggccgatc gaagtcgaag ccgccccgag
    13081 ggcgacgaag agaatcgtga accccagaac gaaggcgagc gagttggtca ggagaacccc
    13141 tttgcgctct tcacgatcgg tgatgcctcc cgccaaatag gaaaaataga gggggagaat
    13201 cggtagaata cagggactga tgaaactgat gatcccttct aaaaacgctg tcaggtatgc
    13261 cattggtcat atatagcaca cgttgaaggt tgttcacaag agcagaaaaa gttggtacag
    13321 tcgattcatg gtcaagaaca cccccaaagt acccaccttc gatgagtact accgcgtatt
    13381 ctatcaggat cgctgggaat ccttaaaaac cgctctcctt gaagaacgca agccggtcgc
    13441 ctataccgaa gggctgctcc aaccctacta tcttgatgag gcatcgattg ccgcggcccg
    13501 ggcgttgggt gtcagcgaag gagaccgggt cttggacctc tgcgcggcgc ccgggggaaa
    13561 gacgcttgtt ctggcgactg cgctgaaggg atcgggcagc ctgacggcca acgaccgttc
    13621 ctccgcccga agggcccgcc tgcgggatgt gatcacaaac catctgccgg aacaggatcg
    13681 ggccaacatc acgatcaccg gccatgatgc cgcgaagtgg tccctctacg aacaggatgc
    13741 gtatgacagg attctgcttg atgcgccctg ttccagcgaa cgtcatgtcc tctcggatga
    13801 gaaggctctc aaagattggt caccgtcaag gcccaagcgc cttgcagtca atcaatttgc
    13861 gatgctcgcc tccgccctcg aggcggtcgt cccgggcggg ctgattctct actccacctg
    13921 ctcgatcaat cctctggaga atgaagaggt catcgccaag cttgagaagc gaagagcggg
    13981 gcggtttgaa gagctgcacc tcgaccgggg agagaaacga tccttcggtt cgatcatcct
    14041 tcccgatgtc tcggatggaa agggaccgtt gtatatgtgc ttgattcgga ggctcacatg
    14101 agttgtgttt ccattgttcg gtgtgaatcg tatgaacctg ccttggtgga caaggcggtc
    14161 agggaggcgt gcgcctctgc ggggatgccg gatgtcaacg gcaagacggt tctgctgaaa
    14221 ccgaacatcc tctccgattc caaagaggag ctcggcatca ccacccacag ccaggtcgtt
    14281 cgaagtgtca tccgcttgct gaaggagcag ggagccaaac ggattcttgt cggagattcg
    14341 ccggggctcc ataccccgaa ctttcgaccc cgagccagcg ggatctggaa ggtaatccgg
    14401 gaagaagagg tcgaatgggc ggatttcacc gaccgtccga ccacctatcc gatcccctac
    14461 gcccgcggca agaagcttcc ccttccctcc ctgctggagg aggtcgacct cctcttctcc
    14521 ctgccgaaga tgaagaccca ccaactgatg tacgccaccg gtgcgatcaa gaacctcttc
    14581 ggcctggttc cgaacctcca caagagcccg tgccacttga gctttccgac ccgggagcag
    14641 ttcgcctccc tgatggtcgg catcgccacg gtcgccaaag cctccttcgc cctgatggat
    14701 ggaatcatcg cgatggaagg ccccggtccg gccaacggct caagcctgcc gatgggcctg
    14761 attctcgcct cgacggatct ggtggctctt gactatgccc aggctgagat catggggtat
    14821 accccgaccg atgtcccgat cgtcgccgag ggattgaagc gggggttggg aaaagcgcct
    14881 agcgaatatc cgaacctcca aacgcatgac ctgatcaaga ccgactataa gcggattgaa
    14941 gtacaaaaga agacccgttt cttccacgcc ctgattctcc ccttctttgc cggacctttc
    15001 gtccgctggc gggtcaaacg ccagcgcaga gcccccttct tcctgaagga agagtgcatc
    15061 ctctgtaaaa aatgcatcca aatctgtccc gttgatgctc ttaggattga agagaagcat
    15121 atagtgattg acgaaaagcg gtgcatccgg tgttattgtt gtcacgaggt gtgtcccgcc
    15181 tccgctatcc tggtcgatga ggagattcct tcgtgacgtc catccactgt ctgattacga
    15241 tcgctttagc cgtccttccg atcagcgagc tcagaggagc gattccctac gcctacttca
    15301 acggcgctcc tctcttcctt gccgccctga tcggagttct ggcaaatctt ctggtcgctc
    15361 cgatcgtcta tctctttttg ggatcgctgc acagggtgtt ctaccggatt tgggggtggt
    15421 atcgctcctt cttcgaccgc ttcgtcgagc gggcacgaaa aaaggtcgag catcacttct
    15481 ccaaatgggg atacatcggt cttgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnngcctc ctgtaaggat catgagaaac gagggttcgt tcatctttcc ggtatcgacc
    15661 ttggctttca gctccaagag gtgttccgcc gcctcttcga tcgccttagt accgagcttc
    15721 acttcgatcg gcgcccatct gccgtcgttg aggcagacga cggtatccga ttccagcccg
    15781 ctcgcatccc gataatggaa gacctgccca tcaatggcat ccgcatagat tcgaagatct
    15841 ctggtgcaga gggattcgaa gagaaagccg aaatactcaa aatgctcaag caatcggaag
    15901 ggggtaagcc gcatcacggc ggttgcgatc gaaggatcta cgaaatgccg tttcactgaa
    15961 cttctgatcg ccgtcttgga tcgcaacgcg ggattccagg caggaagatc ttccgtgaca
    16021 aacatccgat ccaatgcaac gagatactgg ctgatcgttt tttccgaaat gcttgcatcg
    16081 gcatcgccca gagcaatatc atcacggatt gttctcattg tggccgtcgt cgcgatgttt
    16141 cgggcatagg aacgcatcag agcccgtacc cgggcgggat tcttttccac gccatccacc
    16201 cgggaaatat ccgcattgat gatcgcctca acataatcaa ccgcatggcg caaggcgatg
    16261 ctttcatcat cgcctatgga agccggccaa cctccccgta ccagtgcgga agcaatgcct
    16321 tctatggaga gatctgaaac cgcttcgaca ttgatgtgcc cgtcaaacaa tgatttcagc
    16381 gaaaccgacc cattcgactc caacgactca aacaggctca tgggacgcat catcaggcgt
    16441 gaaatccgtc ccgttccggt gtgctgcacc gcatcatcct tcggtaccgc cgaccctgtc
    16501 aggatgaact ggcccggttt tccccgctgg tcgaccataa accgcacagc atcccacaaa
    16561 ataggtgcgc tttgccattc atcgagcaga cggggcgtct cccccttcaa gagaagagag
    16621 ggtttcgtgt cggctgccga gaggtatgag acggtcatat cgggatcctg catgaacaat
    16681 tgactttttg acgctcttgt cgcggtcgcc gtttttccac accattttgg gccttcgatc
    16741 agaaccgctc cacttgatcg aagtctttcc tgcaaaagtc gatctgcaat gcgcttgaga
    16801 tatcgtcgtc cttccatgtg gaatcctccc cttacaagca ttgtacccct aacttccgac
    16861 aatatcaatg ttttacttcc gataatatta ctattttact tccgatannn nnnnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnact tccgatatta ttactatttt acttccgata
    17041 gtattattct gcttcatggt gaaataaaaa cactgaacca agcatttcaa aaatgaatta
    17101 ccggtttccg gtagtcgctt gagagttaaa ctcttggaaa atatgaagat tggctagttg
    17161 ctgttggtta gggtaacggt aatggtgctt ttgtatctgc cttcagcggc ctgattgacg
    17221 aaggtttcat ctattcgacc catgtaaatg gttttcgtat gctctcctgc taccagatta
    17281 ttccattgaa tcggagagtc cttcgtgatc cgggttgtgg tatcccccca gtagaggttg
    17341 tagggaatcg tgggaccggg tgtctcgtga agaagatgaa acccgttcca gccgacaggc
    17401 gagtcgtccg tgaacagtac ctccacgctg atcttctcac cacccgttcc gttctcaacc
    17461 accacttcca atgttgccgt attgatctgt tttacctgag ttccggtaaa ttggctcaat
    17521 atgatgggaa catcctcact ctccagcgaa aagagcgtat ggatgaccgg atccgatatc
    17581 ggttcatccc cgtagacgat tcccccgttg ccgcctcctt ggccgggttc acccccgctc
    17641 atcgctccgc cggggatgat cggggtcgtg gcatgtccgg tattggttcc cgaggggctt
    17701 gcgactggaa cgtatgcgcc tttgtactga tcgttttgac ctgttttagg cgttgtgcca
    17761 ttcatgtagt catacatatt gctgttgggt tgattggcgg tagccaaccc aaagcagggg
    17821 ttgaacgggt ttttgaatgc atagggagtg ttcaggaaaa acccgcttcc ggggttgccg
    17881 gaattggctg ttgcatggat tccacctttc gaattgatgt cggcattggt attgatgaga
    17941 aaaatatcga caagatacgg ctgggcggag gtaccttgca cgtttgcgat attacctgct
    18001 accgccactc ttggttcact ccatgattgt gaaatgtatc ccggattgct accttcacga
    18061 taggctagaa agatgacatg gaacgtaaga tctctttctt cagggtcgtt tggatgattc
    18121 caggaaaaaa ggccggtcat ctttatgggc ggtgaggcca catcgggaga aagcgtgatt
    18181 accggtgtat agatgttccc atcatccgac caaatcctga attgaccaag cttggtgccg
    18241 accatcgtat cactggtaaa cggtggaggc gggcgtttga aatacagact tgtttccggt
    18301 tcatattccg catagaccgc cgcacttgcg gacaccatcc ccgcgataag gatgatgaac
    18361 agagaaagaa tgagtcttgc ctgcgtttgc aatcgacaca tattaaccta taacgactat
    18421 ttcaatcgct attggtcaga gtatatgtat ttcctttccc ggtgtctatc catacgacag
    18481 gatttcttcc ggccgaacgt gcctgtcttg atcaggggtt ctggatctcc acgctgatcg
    18541 tatcggaata cgttccgctg gcaagcgaat cgattgtgtt ttggttgatt ccgccgatcc
    18601 ggatcgtttt cgtgttgctg gagccgtttt ggaggttgtt ccacgtgatg gcctctccct
    18661 ttaccacctc cgtcgaatca aaatagagct tgaagggaat cgggttgggt ccgctttcct
    18721 cgggaagcat cctgaattcg gcggatgtgg atacgggcgt caaatcggtg aagattacct
    18781 tttgggtgta gttggctccc gccgttccgt tggagacggt tatgctcatc gtgtttacgt
    18841 cttttctctt tgagccgatc gcatcggcaa ggttgaactc cgtcgattgg ttggtaagga
    18901 agacgctgta gctgaccatt tgtgggggat cgccgtacgt cattcccggt tgtccgggat
    18961 tatccgggtc ggtatacgcg gatgattgaa cgatgggggt gctgtccgcc ccggcgacac
    19021 cgttgacgct cacatatgat ccgtgtgagg ggttcggttc tccattgttc atcatccatc
    19081 cgacattcat ccaataatca ttggcgctga cgaagctgaa gaccgggttg aaggttatcg
    19141 gcaaggtgta tacggcgttc aactggaaat aacttgccgg tcgccaatac gtcgttgtgt
    19201 gctgataggt tgaattcgtg ctcatgaggt agagctcaac ccggaacgga ttggcatcca
    19261 ccgtgtttgc aaaccacgag acaatcgggg aatgagggga ctggaccact ctgatgaccg
    19321 gtgttccggg atatccataa ggatatgcga cgctcatgat gtggaacgta tgggttgcat
    19381 tttcctcaaa cggaccgccc ggataagatc tcatcggtcc tctgagaatt gccgaatatt
    19441 cgacttcatc ggtggagccc catgtcggtg catagatctt gtcaccgtcg gtcgtcgtga
    19501 cgataagggt gcccagcttc gccccgaacg tcgtgtcgct tgtaaagggg ccgggtttgg
    19561 cctgaaggac gagatacggc tcaggggtat atacggcggt gatcgctccg aagaggagtg
    19621 ccggcacaag caggagcatc aagagaacaa ctgtgtatct gacagaaccc atacgatcac
    19681 cctccttcat atatttattt attattcaca acctcctacg gattccttgt catactatac
    19741 caaaccagta gtatattgca agttctgtca tgggtattat tgcaatttta ctgtaattct
    19801 tagtttttcc aatta
//
LOCUS       DAOSLI010000033        19418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3067_length_19418_cov_24.087745, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000033 DAOSLI010000000
VERSION     DAOSLI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3067_length_19418_cov_24.087745"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..231
                     /locus_tag="PLF67_06025"
     CDS             58..231
                     /locus_tag="PLF67_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89605.1"
                     /translation="MTIHEQIVAQYEAYLEENRKFTEKGVKAAAARARKALAELGKLA
                     KDRRKEIQEEKNA"
     gene            complement(266..2914)
                     /locus_tag="PLF67_06030"
     CDS             complement(266..2914)
                     /locus_tag="PLF67_06030"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOE89606.1"
                     /translation="MKAVELSKAYDPKDFEQRIYQKWLDSGSFEPVEGSGKAYTVVMP
                     PPNVTGILHMGHALNNSLPDILIRYHRMMGIPTLWVPGTDHAGIATQNVVERQLAKEG
                     LTRDDLGREKFLERTWKVKEEHHRIIKEQLMKIGCSCDWKHERFTLDEGLSNAVREAF
                     VTLYERGLIYKGEYLVNYCPSCGTALADDEVEHQSRNAAIYRIAYPFSDREGEIVVAT
                     TRPETMFGDVAVAVNPNDERHNHLIGKCVHLPLTDRIIPIIADDFVDIEFGTGFVKIT
                     PAHDPNDYAAALRHDLPKVNILHADGTLNDEVPKEFRNLKADDARSKVVEALKTGGYL
                     KSEEPYTHEVGHCYRCHNVVEPYLSNQWFVRMDGMAKKALKALEDGKIVFHPKHWENT
                     YTHWLENIHDWCISRQLWWGHRLPVWECEACSHLIVSREDPTECPKCKKTDLVQESDV
                     LDTWFSSWLWPFSTLGWPEETADLKRFFPTDSLTTGYDIIFFWVARMIMASLEFLDEV
                     PFKDIYITGLVRDKQGRKMSKSLGNGIDPLEVIDQYGADAMNFTLSYMATQGQDILID
                     MDTFRLGSRFANKVWNAARFVLLNIGGVTLQNIEEIKLNTIDKWIYHRFNQTVRKVHA
                     AMNLFKFNEGSQAVYDFFWNDFCDWYLEAAKEGMYSKEEEPKNRQISLLLDLLERSMA
                     LMHPYLSFITEEIYSKLPNKKGLLINHPYPEYDEALVFPEEHTVFTRLQEAVTSIRAV
                     RSELQIPLDRKVRVAIQSDDSFIGASFFKAHERLLALFTNALSVEVDREVEIHGALPV
                     AGNGYQSFVFVSDAIDVEKEISKLEGEIEKTKKNLEGTLKKLANEGFLRNAKEEAIAK
                     EQAKRVEFEEKLQKSEQHIALLKTLV"
     gene            complement(3000..3536)
                     /locus_tag="PLF67_06035"
     CDS             complement(3000..3536)
                     /locus_tag="PLF67_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOE89607.1"
                     /translation="MCVDDQLLNTYLDQELKEPWKTQVEEHLSYCNACRLRLSALQEI
                     QNRLASATLSDDEIAPSKERVLTYFEKNRFSGESTKQLFWHKRVQVKLMPAILTSAAA
                     FVVVFIASFVLFSSNPEQRQEILPGVADPIDSTHIRQVSDAPRPSLDSFSLEQIINHL
                     DKLGYAVKLELKAITPLE"
     gene            complement(3540..4058)
                     /locus_tag="PLF67_06040"
     CDS             complement(3540..4058)
                     /locus_tag="PLF67_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOE89608.1"
                     /translation="MEIQSNDERAFRGVYEEVFPIIMRVAYHVTNNRDLAEEICQEAF
                     IRFFDKGMLFPSSDDAKYWLIRVTKNLAINQVKRKVREQGMVEKLKQMPTSRANTTDG
                     EHVLIREETRAEVQEAISQLPEKFRLVIVMKEYTDMGYREIAEVLRISESNVKVRVHR
                     ARKMLESILNRE"
     gene            complement(4086..>4280)
                     /gene="cysS"
                     /locus_tag="PLF67_06045"
     CDS             complement(4086..>4280)
                     /gene="cysS"
                     /locus_tag="PLF67_06045"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607341.1"
                     /note="catalyzes a two-step reaction; charges a cysteine
                     by linking its carboxyl group to the alpha-phosphate of
                     ATP then transfers the aminoacyl-adenylate to its tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOE89609.1"
                     /translation="LGFDEITKPKDVAANDEALRLLDERNEAKAAKDWGLADRLRAEL
                     LEMGYVVKDLPSGSVLEEKM"
     assembly_gap    4281..4380
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4381..5348)
                     /gene="cysS"
                     /locus_tag="PLF67_06050"
     CDS             complement(<4381..5348)
                     /gene="cysS"
                     /locus_tag="PLF67_06050"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOE89610.1"
                     /translation="MALRLYNTMSRSIEEFIPITAKKAGMYCCGPTVYNYAHIGNLRT
                     FLFEDLLKRVLIASGYEVTHVMNVTDVGHLSGDGDDGEDKLEKAAREKKKSAWEIAKY
                     YTDAFFRDFDDLKMIRPSTTCKATDHIQEMIDLILRLEEKGHTYQSGGNVYFSIDTID
                     DYGKLARLELDRLKTAVREGVEEDEHKKNPKDFVLWFTQSKFGEQEMMWPSPWGRGFP
                     GWHIECSAMSMTYLGDQFDIHCGGIDAIPVHHTNEIAQSEAATGKKWVNYWLHGEFLL
                     DETGKMSKSKGEFLTLSLLKEKGFDPMDYRYFTLGGHYRSQLRFNF"
     gene            5400..6305
                     /gene="murB"
                     /locus_tag="PLF67_06055"
     CDS             5400..6305
                     /gene="murB"
                     /locus_tag="PLF67_06055"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOE89611.1"
                     /translation="MPINVSNRVEKINLEQDVNLAKYSTIKSGGTADLFCYPTDIQEL
                     RAVLAFARARSIPVTVLGGMSNTLISDEGIEGLTIGTVKLNEIKKRGNLLSVLAGAQL
                     EETINVAIEEGLGGLEHFYGIPGTIGGALWGNAGAHDHQISETVLFADFLDLHGNLFR
                     KQIIQSEFSYRMSPYKGRSDLILYEVHLALNPISDTHTLKTIALGYKQRRIEQQQFRY
                     PSLGSIFKNPPGHKAGALLEGLGLKGTRVGGAQIAVHHANMIINVDGSATSTDIRTLI
                     DMAKEKVFQKYGFTLEEEITFVGRW"
     gene            complement(6283..7305)
                     /locus_tag="PLF67_06060"
     CDS             complement(6283..7305)
                     /locus_tag="PLF67_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89612.1"
                     /translation="MRRFSFLCLLLFASAALWAEANLLLCFEPLQKTFKASVETAGIS
                     LDLSYRFASEMPAPSVVLGPFRIKTNRIAVRNTTLHPLFANELFETRLEHPQWGRLSL
                     FRADGRHIGLGFEWGAFTGIFIHLPILEPKNVFLPHRKHQGLTVFLRYSRGPVTVHCI
                     GSAHTPLSLQGSVVLHAGAFTIRSVLDSSAFPYRLTLGLEGGKASLSHEFSVGREPIY
                     SGSEQVLRVSQKSNVATSIGSFVIGLDYLHTLQTKEDRMVRTDLRIRATIKHASNRIY
                     LVWREREGWSFELKGGNGSLVLSKEGVAYEFAAHFEQGEVRISLTSKGVFSIAYTGRF
                     TIGQRR"
     gene            7434..8768
                     /locus_tag="PLF67_06065"
     CDS             7434..8768
                     /locus_tag="PLF67_06065"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOE89613.1"
                     /translation="MVILTLNCGSSSAKYQVYDWEEKEVLAVGVVERIGLEYSTIEHK
                     AVGKEEYTAEFSSPTHKEAIELIIRMLLDKEYGVIGDLKEIGAVGHRVLHGGEAFKKS
                     ALITDEVIAQLKSYIHLGPLHMPANIMGIEGAVKAMPDVPQAIILDTAWHQTMPEDAF
                     MYAVPYEWYTKYNVRRYGFHGTSHLYCAKRAAAILGKKNKDTNVIICHIGNGASVSAV
                     KNGIAVDTSMGLTPLEGLVMGSRSGDIDPAIIPYMMENTGMSAREMDTVLNKKSGLIG
                     LCGKSDRRDVQKSAREGDKNAQLAIDMEGRRIGKYIGSYATLLGGRLDAIVFTAGVGE
                     MGPHIREKACEGLEIIGAKLDLKKNKTSLSRNGEFEISTDDSKVKILVIPTDEELVMT
                     EDAFALMNGTYDVHTNFTYSFESREYVNKARAAGLKRDLEKNPALKDILAIK"
     gene            complement(8777..9385)
                     /locus_tag="PLF67_06070"
     CDS             complement(8777..9385)
                     /locus_tag="PLF67_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89614.1"
                     /translation="MSDNKKPVAGVDIVDRRYVVCLLNEEGKAPRYYSGRSDTPSGQE
                     KFFSLIEGRTVVMPPDPLSTTAVHRFGEAAVSIDRGYEAYRISGIKNGKRMARFVALL
                     AFHQGVKNTALTKKEQEEMFAKQQEELDRLIEFTDSVGPLMERLINGNATPSDVEEMI
                     RLEVESRYPKNGNPPLVEDIDLPDPKEDSFFARLYRYLKAKT"
     gene            9489..10013
                     /locus_tag="PLF67_06075"
     CDS             9489..10013
                     /locus_tag="PLF67_06075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOE89615.1"
                     /translation="MKQNERLLVVVDMQNDFVTGSLGTEEAKRIIDGAVAKINAEKGS
                     VAYTLDTHFDDYLSTSEGRSLPVEHCIKGSEGHKLIPELRSPLKDAKSFEKHTFGSVE
                     LAEYIRDNPQIREVELFGLCTDICVVSNALMIKAMRPELVVKVDGRISAGTTVEKHNA
                     ALDTMESCQIVVTR"
     gene            10086..11276
                     /locus_tag="PLF67_06080"
     CDS             10086..11276
                     /locus_tag="PLF67_06080"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOE89616.1"
                     /translation="MLQPLSYVLVTPYTVAKSRTGGVLSRLLSRISLELVGAQMVALD
                     KETTEAFAQIIENRNLAACCGATRDILGSYIRQNLGPSDDGSLHRSLFLVFRGDNPTK
                     ELSTVCGTFQKDADDLEAVTGESIRDTYADLIYTDESQQTLRYFEPAVITASEQKEAE
                     AVIRLFAKWLPTQNNLIHNRSEEYYKGVERTLVIIKPDNWRYASSRPGMIIDMFSRSG
                     LRIVAIKVLKMSVAQAVRFYGPTKEGLKKRLAPIYGMQARELLEREFNIPLTEELEKT
                     LTESFGDMYGEEQFERIIEFMAGIKTYERPEEEWEEPGLVKSMILVYEGKDAISKIRT
                     ILGATDPTKAAAGTIRREFGSNICINAAHASDSVESAVREMGILEVEKNHLGGVLQHY
                     VAHR"
     gene            11362..12243
                     /locus_tag="PLF67_06085"
     CDS             11362..12243
                     /locus_tag="PLF67_06085"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOE89617.1"
                     /translation="MTIAPFLHMLPILVVILSGYVMGRLLPIDSKTLATVAADFFMPA
                     LLFISMATTDLEGSLVSSLIGSSISITAMLLIVAFIWAKLAGQEPSKTVPSLVFMNSG
                     FLAIPLMKLYGGNLGMNMSVIFDFTQSFAMFTVGVFIITGGLKASSVKAAFASPIIWA
                     TVSGLLVRTLAIPIPETIREIFLYAGDGAPALASFALGVSLKDFKLTLKVELISALFV
                     RFVGGFLIGLLAANLFGLQGMAKVVVTVTAALPSAIFTSVLPIRHNKDANFATTMVLL
                     STLLGAFTIPLSFALMG"
     gene            12349..13650
                     /locus_tag="PLF67_06090"
     CDS             12349..13650
                     /locus_tag="PLF67_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE89618.1"
                     /translation="MLTRRVKFGFGIGDLGGNLFFTIIGFYLLFYFTDIVGLNPALSG
                     TAVMIGKVWDAITDPITGYYSDRTKSRFGRRRPYIFIGAIFSFFFMIQLFTPVSMKNE
                     MSLFLYYTLVYSLLNTAYTLVNIPYTALLPELTDDYHERTILTGYRMSFAVLGTFAGA
                     ALVMPILNATSWPVMGGVMGGIMLASAMITVFAIREPKEHSKPGEDGFLSTFAEVLKE
                     KVFLQALLPWTFFITGTMMIQGGLVYYFTYIYGNKDLFQLALVGLLSFSLLCIPFWVS
                     VSKKIGKRKVYMIGMSIVSVGVLLFTTLGAVFGPIFGIVVMSLAGIGLSTHYVMPHSI
                     LPDVVEYDAHTNKKGRREGVFSSIWTFSSKIGQAFALALNGWILAIFGYQGGSFLPQT
                     KPGIILIVGPLPILWYILGLLILRHYPIDQAYYNEMIKRGE"
     gene            13640..14893
                     /locus_tag="PLF67_06095"
     CDS             13640..14893
                     /locus_tag="PLF67_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89619.1"
                     /translation="MANKDRFPAPAYSPLARMIVPLFGKLYLRLALGVSTIVLPEGEI
                     LRNELRRFEERRSSLILAFRHTAVEDAPTLLVGVKHPHLRFLYGRDVVNWAGWPTKIL
                     FPALGFIAVENRGNNREGAQYLRKEVQNPRFPIALAPEGQVTYHAYTCASIEMGAANI
                     ASWALEGPREEVVILPIGIGYRYAKDNDRFLLDLITRWEKEANVAVDRSLPPNEQIRA
                     IGYETLKLVNTFWNLNLTLKGSFIEQRDGLCDALLRYSESLGGLEDSSGSIIDRLFRV
                     RYKAVSVLNDTDRSLLSEDERRLHDEEVTKQKISDHAAQVVDVLEYIDLNYLEGKHAV
                     QRSIEVMLSLLDVLNRLQGGMINSRFSPKSKKAFILGGTPIEVRKSFGHITGRKERLN
                     AINQALEEGLNNVSLTLEEIMFQST"
     gene            complement(14859..15539)
                     /locus_tag="PLF67_06100"
     CDS             complement(14859..15539)
                     /locus_tag="PLF67_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE89620.1"
                     /translation="MSFAKRFVNSVLRTYFRLFFKIDRSELVKVPLEGPLMMIVNHTS
                     VFEGPMLYAYLQPRAMHALAKKELWKNPVTRYLMNLWESIPVDRGNVDRQAMDAIFTV
                     LDKGEIFAIAPEGTRSKDGTLQRGKSGIAFIAYKNRTPMVPIAVTGFENFRRNLLRLR
                     RTRITIKVGEVFRPIAQGGRLDAEGRQALADEMMVRIASLMDERHQGYYKTFDPTFTL
                     TETLSLQG"
     gene            complement(15577..16680)
                     /locus_tag="PLF67_06105"
     CDS             complement(15577..16680)
                     /locus_tag="PLF67_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOE89621.1"
                     /translation="MITYICKQCGKTYPLSTKRYACECGSLFHLSYPKTAPAFGSLGG
                     DRFLWRYRSALPPFEEKTIKRVTMGEGGTPVIRLEEHLYGKADYLMPTLSFKDRGAVM
                     LAALMVEIGVKRCAIDSSGNAATAVAAYAARAGIATEVFVPASTSAKKLTQIEAHGGR
                     IHAIEGSRDETARATIDYVDKSEIFYASHIFNPFFWEGTKTYVYELYERFGSLPEVLI
                     VPVGNGTLLMGVYLALQELKAWNLIDSHPLVIALQAAGCAPLAQAYGQGSLSPQSVLV
                     SPTLAEGIATGEPARGKEILQAVHDLGGRFVTVTEEEILKAHGDLAKKGVYVEYTGAA
                     NYAGYRVALKEDPMLADKTSLIPLTGAGLKSAH"
     gene            complement(16677..17432)
                     /locus_tag="PLF67_06110"
     CDS             complement(16677..17432)
                     /locus_tag="PLF67_06110"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOE89622.1"
                     /translation="MNAIGIIGGVGPMAGADAAQKIFENTNAATDQEHIDLYLTSTPR
                     SIGDRTKFLLEGGSNPAEGLYKNALQLIGLGASTLIVPCNTAHAPPIFDPLRKKLRDS
                     HPDITLLHMIEETAKHIAKRFPEPTTIGLLATKGTHALKTYPNALLAYPHITLIEPDR
                     ESRERVHDAIYNQRYGIKARAPVSREALTILIEEAYKLHKQGAEALILGCTELPLALT
                     RETISLPLIDPTVVLARSAILHTEPAKLKDEVE"
     gene            17508..17891
                     /locus_tag="PLF67_06115"
     CDS             17508..17891
                     /locus_tag="PLF67_06115"
                     /inference="COORDINATES: protein motif:HMM:NF025825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOE89623.1"
                     /translation="MASIRSIRERMQELYVYDLYGFRSENKALAGVLYPHEKINSFAV
                     GLHEGLKKMLVVTTHRLIIIRSSFGKTPDVQAVKRELIEEPSYTKRFFNSELSFKLHG
                     ETFTFTMVSRRVLELFVWAVEQPLE"
     gene            complement(17940..19313)
                     /locus_tag="PLF67_06120"
     CDS             complement(17940..19313)
                     /locus_tag="PLF67_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOE89624.1"
                     /translation="MADEKKKTPVKKEAEVKKEPEVKKEAPVKKEEAPVKKGVLSWYF
                     NFNLLYRILIGLVLGALLGIILGEKMLWIAPLGGLFVRLLKMIMMPIIFSTLVVGAAS
                     ISPAKLGKVGLRVVLFYLVTSAFAVFVGLVMGSIFRPSAEMGALVAGAAGKAAAKTPV
                     STVLLNIIPTSVAQAIVNETVLQVIFFAIIFGIGISYLNISKDKRLKTAGETLIVVFD
                     GTAEVMMRMVTGIMQYAPIGVLALVAEVFAKQGSKVAGSLAVVTIACFLGYAIHVVLV
                     YGGMLMVNKLSIKRFFNDAKDPFITAFVTRSSNGTLPVTIEAAKRLGIPRDVYSFSLP
                     LGATINMDGTAIYQGVCAIFISLTVTGANFTLGQMGMIIVTATLASIGTAGVPGAGAI
                     MLLMVLESVGLPVVEGSAVAGAYAMILGIDAILDMGRTALNVTGDLVCTTVVSKNMGL
                     LDQKAWE"
BASE COUNT         4401 a         5302 c         5072 g         4543 t
ORIGIN      
        1 gtttgcgttg gcattaaatc gtggtaaaat aacgcaacat agcaaaagga gttttctatg
       61 acaatccacg aacaaatcgt tgcgcagtat gaagcctatt tggaagagaa ccgaaagttc
      121 accgaaaaag gcgtgaaagc tgcggcagca cgcgcccgca aagcccttgc tgagctcggc
      181 aagcttgcca aggatcgccg caaggaaatt caagaggaga aaaacgcata atgagtgaga
      241 ggcgcccatc cggcgccttc ctctttcaga cgagagtctt caacaaggcg atgtgttgtt
      301 cactcttctg aagtttttct tcaaactcga cgcgcttggc ctgttccttt gctatcgctt
      361 cttccttggc gttccgtaga aatccttcgt tggcgagctt cttgagcgtc ccttcgagat
      421 tctttttcgt cttttcaatc tccccttcca attttgagat ctctttctca acgtcgatcg
      481 catcgctgac gaagacgaag ctttggtagc cgttgccggc aacgggaaga gcaccatgaa
      541 tctcgacttc ccggtcaact tcaacgctca gcgcattggt gaagagggcc agcagtcgct
      601 catgagcttt gaagaacgag gcaccgatga atgaatcatc gctttggatc gcaacccgca
      661 cttttcgatc aagaggaatc tgcagttcac tccgcaccgc cctgatcgag gtaaccgctt
      721 cctggaggcg ggtaaagacc gtatgctctt caggaaagac caacgcttcg tcatactcgg
      781 gatacggatg gttgatcagc aagcccttct tgttcggcag cttcgagtag atctcctcgg
      841 tgatgaagga aaggtaggga tgcatcaacg ccattgaacg ctcgaggagg tccaacagaa
      901 gggagatctg ccgattttta ggctcctcct ctttggagta catcccctct ttcgccgctt
      961 ccagatacca gtcacagaag tcgttccaga agaagtcgta gacagcctgg ctgccttcat
     1021 tgaacttgaa gaggttcatc gccgcatgga ctttccggac cgtttggttg aaacggtgat
     1081 agatccactt gtcgatcgtg ttgagcttga tctcctcaat gttctgcagg gtgacgcctc
     1141 cgatgttcaa aagaacgaat cgggcggcgt tccagacctt gttggcaaag cgactgccaa
     1201 gacggaacgt atccatgtcg atgaggatgt cctggccttg cgtcgccatg taggaaaggg
     1261 tgaaattcat cgcatcggcg ccgtattgat cgatgacctc caagggatcg atcccgttgc
     1321 cgagcgattt gctcatcttt cgtccttgct tgtcgcgaac cagaccggtg atgtagatgt
     1381 ctttgaaggg gacctcatca aggaactcca agctcgccat gatcatcctc gccacccaga
     1441 agaagatgat gtcgtacccg gtggtcaagc tgtcggtggg aaagaatcgc ttcaagtccg
     1501 ccgtttcttc cggccatccg agggtggaga acggccagag ccacgagctg aaccaggtat
     1561 caagcacatc cgactcttga acaagatccg ttttcttgca cttggggcac tcggtgggat
     1621 cttcccgact gacgatcagg tggctgcatg cttcgcactc ccagacggga aggcggtgcc
     1681 cccaccagag ctggcggctg atgcaccagt catggatgtt ctcaagccag tgggtgtagg
     1741 tgttttccca atgcttcgga tggaacacga tcttcccatc ctccaacgct ttgagcgcct
     1801 tcttcgccat cccgtccatc ctcacgaacc attgattgga cagatacggc tcgaccacgt
     1861 tgtggcagcg atagcagtgt ccgacttcgt gggtgtacgg ctcctcactc ttcagatatc
     1921 cgcccgtttt gagggcttcg accaccttcg accgggcatc atccgctttg aggtttctga
     1981 actctttggg cacctcgtca ttcagcgttc cgtcggcatg gaggatattg acctttggca
     2041 gatcgtgccg aagggcggcg gcatagtcgt tgggatcgtg agcaggcgtt atttttacga
     2101 acccggtccc gaactcgata tcgacaaagt catcggcgat gatcggaatg attctatcgg
     2161 taaggggaag atgaacgcac ttgccgatga gatggttgtg ccgctcgtcg ttggggttca
     2221 cagcgaccgc cacgtctccg aacatcgtct ccgggcgggt cgtggcgacg acgatctccc
     2281 cttcccggtc gctgaacgga taggcgatcc ggtagatcgc cgcattgcgg ctctggtgct
     2341 cgacctcgtc atcggccagc gcggtcccgc aggaggggca gtagttgacc agatactccc
     2401 ctttgtagat caaaccccgt tcatagaggg tgacgaacgc ctcgcgcact gcgttgctca
     2461 atccctcgtc gagggtgaag cgctcatgct tccaatcaca ggaacagccg atcttcatca
     2521 gctgctcctt gatgattcgg tggtgttcct ctttcacctt ccaggttcgc tcaaggaatt
     2581 tctcccttcc gagatcgtcc cgggtcaatc cctccttggc aagctgacgt tcgacgacgt
     2641 tctgtgtcgc gattcccgca tggtcggttc ccggaaccca aagggtcgga atgcccatca
     2701 tccgatggta gcggatgagg atatccggca ggctgttgtt caacgcatgg cccatgtgaa
     2761 ggattcccgt tacgttcggc ggaggcatta cgacggtata ggcttttcca ctaccctcga
     2821 ccggctcgaa gcttcccgag tccaaccact tttggtagat tcgttgttca aaatcttttg
     2881 ggtcatatgc tttgctcaat tcgacagcct tcatcacaac ctccgcgtgt gaggctatcc
     2941 tatcaaaata gtaaaggctc tacaagaatg cagagccttc gtccgatatg tgcaaggggt
     3001 tattcaagcg gggtgatcgc cttcaactca agcttcaccg catagccaag cttgtcaagg
     3061 tgattgatga tttgctcaag gctgaaggaa tccaaggaag gacgcggcgc gtcgctgact
     3121 tgtcggatat gtgtggaatc gatcggatcg gcaacaccgg gcagaatctc ctgacgctgt
     3181 tcggggttgc tggaaaagag gacgaatgag gcgatgaaga ccaccacgaa cgccgctgcg
     3241 ctggtaagaa tcgccggcat caattttacc tgaacccgtt tatgccaaaa aagctgcttg
     3301 gttgactctc cgctgaaccg gttcttttca aaatatgtga gaacccgctc ctttgacgga
     3361 gcaatctcat catcgctcaa ggtcgccgag gcgagtcggt tttgaatctc ttgaagagcg
     3421 gaaaggcgaa gccggcaggc gttgcagtag gaaagatgtt cttcgacctg cgttttccac
     3481 ggttctttca gctcttgatc caggtacgta ttcaacagtt ggtcatctac acacatgtac
     3541 tactcccggt tgaggatcga ctcgagcatt ttccgtgccc ggtggaccct caccttcacg
     3601 ttgctttcgg aaatacggag cacctcggcg atttcccgat atcccatatc ggtatattcc
     3661 ttcatcacga tcaccaggcg gaacttttcg ggcagctggc tgatcgcctc ctgcacctcc
     3721 gctctggtct cctctctgat cagcacgtgc tcgccgtcgg tggtgtttgc acgggaggtc
     3781 ggcatctgct tgagcttttc caccatcccc tgctcgcgga ccttccgttt gacttggttg
     3841 atcgccagat tcttcgtaac ccggatcagc cagtacttgg cgtcgtcgct tgaaggaaag
     3901 agcattcctt tgtcaaaaaa ccggatgaac gcctcctgac agatctcttc agcaagatcc
     3961 ctgttgttgg taacgtggta ggcaacccgc atgatgatcg ggaagacttc ctcgtataca
     4021 cccctgaacg ctcgttcatc gttgctctgt atttccataa gattaacaac tcatacgtac
     4081 aatcgttaca ttttttcttc gagaacactg ccggaaggca gatccttgac cacatatccc
     4141 atctcaagaa gctcggcgcg aagccggtcg gccagacccc aatccttcgc tgctttcgcc
     4201 tcgttgcgct catccaacag gcggagcgct tcatcattgg ccgcaacgtc tttcggcttg
     4261 gtgatttcat caaagccgag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 tcaaagttga accgcaattg agagcgatag tgcccgccga gggtgaagta ccggtaatcc
     4441 atcggatcga agcctttctc cttcaacagg ctgagcgtaa gaaactcccc tttcgattta
     4501 ctcatcttcc ccgtctcatc gagaaggaac tcgccgtgga gccaataatt gacccatttc
     4561 tttccggtgg ccgcctcgct ttgagcgatc tcgttggtgt ggtggaccgg aatcgcatcg
     4621 attccgccgc agtgaatatc gaattgatcg ccaagatagg tcatgctcat cgccgagcat
     4681 tcgatgtgcc agccggggaa tcccctgccc cagggcgatg gccacatcat ctcctgttca
     4741 ccgaatttgc tttgggtgaa ccaaagaacg aagtctttgg ggttcttctt gtgttcatcc
     4801 tcctccaccc cctcccggac cgccgttttc agccgatcga gctcgaggcg ggcgagcttg
     4861 ccgtaatcat cgatcgtgtc gatcgagaag tacacgtttc ccccgctttg gtaggtatgc
     4921 cccttttctt ccaggcggag aatcagatcg atcatctcct ggatgtggtc ggtcgccttg
     4981 caggtcgtgc tcggtcgaat catcttcaga tcgtcaaagt ccctgaagaa cgcatcggtg
     5041 taatactttg cgatctccca cgcgctcttc ttcttttccc gggcggcttt ttccagtttg
     5101 tcctcaccgt catcgccgtc cccgctcaaa tggccgacat cggtaacgtt catcacgtgg
     5161 gtcacctcat acccgctggc gatgagaacc cgcttcagga gatcctcgaa caggaaggtt
     5221 cgaaggtttc cgatgtgggc atagttatag acggtgggac cgcagcaata catccccgct
     5281 tttttggcgg tgatcgggat gaactcttcg atcgagcgac tcattgtatt atacaggcga
     5341 agcgccatga caagctcctt tggattgctt gacccgataa atagggctaa gatgtcttca
     5401 tgcctatcaa tgtaagcaat agggttgaaa aaatcaacct tgaacaggat gtcaacctag
     5461 ccaaatattc aacaattaag agtggcggaa cggcggatct gttttgctat ccaactgata
     5521 tacaagaact tagagctgtc cttgcatttg ctcgtgcaag atcgatcccc gttacggttc
     5581 tcggaggcat gagcaacacc ctgatcagcg acgaggggat cgaaggtctt accatcggaa
     5641 ccgtgaaatt gaacgaaatc aagaagcggg ggaacctcct ctccgttctt gcaggggcgc
     5701 agctggaaga aacgatcaat gttgcgatcg aagaggggtt gggaggcctt gagcacttct
     5761 atgggattcc cggtacgatc gggggggcgc tttggggaaa tgccggagcc cacgatcacc
     5821 agatcagcga aacggtgctg tttgccgatt ttctcgatct tcacggcaac ctctttcgaa
     5881 agcagatcat ccaaagcgaa ttctcctatc ggatgagccc gtataaaggg cggagcgatc
     5941 tcatcctcta tgaagtgcat ctggccttga acccgatcag cgatacccac acgctcaaaa
     6001 cgattgcctt gggatataaa caacgacgga tcgaacaaca gcagtttcgc tacccttccc
     6061 ttggctcgat ctttaagaac ccgcccggtc ataaggcagg ggcgttgctg gaggggttag
     6121 gcctcaaggg aacacgggtc ggaggggctc agatcgctgt ccaccacgcc aatatgatca
     6181 tcaacgttga tgggagcgcc acctccaccg atatccgcac cctgatcgat atggccaaag
     6241 agaaggtctt tcagaagtat ggattcaccc ttgaagaaga gatcaccttc gttggccgat
     6301 ggtgaaccga cccgtgtagg cgatggaaaa cacccctttg gatgtgagcg aaatccgaac
     6361 ttcaccctgc tcaaagtgag cggcaaactc gtacgcaacc ccttctttgg aaagaacaag
     6421 cgatccattc ccgcccttca gctcgaaaga ccacccttcc cgttcacgcc agacaaggta
     6481 tattcgattc gatgcatgtt tgattgtcgc ccggatgcga agatccgttc gaaccatcct
     6541 gtcttctttt gtttgcagag tgtgaagata atcaagtccg atgacaaagg agccgatgga
     6601 tgttgctacg ttgctttttt gggagactcg gagcacttgc tcgcttccgc tgtagatcgg
     6661 ttccctacca acgctgaact catgtgaaag tgaagctttt ccaccttcca atccgagggt
     6721 aagacgatag ggaaacgcac ttgaatcaag aacgctccgg attgtgaaag ctcctgcatg
     6781 aagcacgacc gacccttgga gcgaaagcgg cgtgtgggca ctgccgatgc aatgcaccgt
     6841 gaccggtccc cgggaatagc gaagaaacac cgtcaatcct tggtgttttc gatgaggaag
     6901 aaacacgttc ttcggctcaa gaatcggaag atggatgaat attcccgtaa aggctcccca
     6961 ttcaaatcca agaccgatat gcctcccatc cgcccgaaag agggaaagcc ttccccattg
     7021 ggggtgttca aggcgtgttt cgaacaattc attggcgaaa agaggatgaa gtgtcgtatt
     7081 ccttacggcg attcgattcg ttttgattcg gaacgggccg agaacaacgg aaggtgcagg
     7141 catctccgag gcgaagcggt aggaaagatc gagggagatt cccgcagtct cgaccgatgc
     7201 cttgaaggtt ttttgcaacg gctcaaagca cagaagaaga ttcgcctccg cccaaagcgc
     7261 tgcggatgcg aagagaagaa gacagagaaa ggaaaaacgt ctcatttctt cttaaggaaa
     7321 agagtttcca tctctacttt atgttgattc ttcgtctcaa taatggttta atactttgtg
     7381 gcacccgtgc caaccgagct ttgtaatgtt atatccaaaa tgaggagtgc ttcatggtaa
     7441 ttttaaccct gaattgtggc agttcttctg caaagtacca agtctatgat tgggaagaaa
     7501 aagaagtgct tgctgtcggc gtggtcgaac gtattggcct tgagtattca acgattgaac
     7561 acaaggcggt cggtaaagag gagtataccg ccgagttttc ttcccctacc cacaaggaag
     7621 cgatcgagct gatcatccgc atgctcttgg acaaagagta tggcgtaatc ggggatctga
     7681 aagagatcgg ggccgtggga caccgggtac tgcacggcgg cgaagccttc aagaaatccg
     7741 cattgatcac cgacgaggtc attgcccagc tcaagagcta tatccatctg ggtccgttgc
     7801 atatgcctgc caacatcatg ggcatcgaag gcgcggtaaa agcgatgccg gatgttcctc
     7861 aggcgatcat tctcgatacc gcctggcacc agacgatgcc cgaggatgca ttcatgtacg
     7921 cagtgccgta cgaatggtac acgaagtaca acgtccgtcg ctacgggttc cacggaacca
     7981 gccacctcta ctgcgccaaa cgagcggcgg cgattctcgg aaagaagaac aaagacacca
     8041 acgtcatcat ctgccacatc ggaaacggcg catcggtcag tgcggtaaaa aacggcatcg
     8101 ccgttgatac gtcaatgggc ctcactcccc tcgaaggtct ggtgatgggt tcccgcagcg
     8161 gtgacatcga tcctgcgatc attccctaca tgatggaaaa taccgggatg agcgcccgcg
     8221 agatggatac cgtgttgaac aagaaaagcg ggctcatcgg tctgtgcggc aagagcgacc
     8281 ggcgggatgt tcaaaaatcc gcccgggaag gggataaaaa cgctcagctg gcgattgata
     8341 tggaaggacg gcgaatcggc aagtatatcg gttcctatgc aacgttgctt ggaggccgcc
     8401 ttgatgcgat cgtcttcacc gccggggtcg gtgaaatggg accgcacatt cgagaaaagg
     8461 cgtgtgaagg acttgaaatc atcggggcaa aacttgatct gaagaaaaac aaaacaagct
     8521 tgagccgaaa cggcgagttt gaaatctcaa ccgatgattc aaaagtaaag atccttgtca
     8581 ttcccaccga tgaggagctt gtcatgaccg aggatgcgtt tgctttgatg aacggaacat
     8641 acgatgtcca caccaacttc acctattctt ttgagtcaag ggagtatgtg aacaaagccc
     8701 gggccgccgg cttgaaacgg gatctcgaaa agaatcccgc cttgaaggat attctcgcca
     8761 tcaaatgagg atcggatcag gttttggctt ttaagtacct gtacagtcga gcgaaaaagg
     8821 aatcctcctt cggatcggga agatcgatgt cttcaaccaa aggagggttc ccgtttttcg
     8881 gatagcgcga ttcgacttca agacggatca tctcctctac atcgctcggt gtggcgtttc
     8941 cgttgatcag ccgctccatc aacggaccga cggaatcggt gaattcgatc aatcgatcaa
     9001 gctcctcctg ttgcttggca aacatctctt cctgctcctt ttttgtaagg gcggtattct
     9061 tcaccccttg atgaaatgcc aagagagcga cgaatctcgc catccgcttt ccgttcttga
     9121 ttcccgagat ccggtaggct tcatatcccc tgtcgatgct taccgccgct tccccgaatc
     9181 gatgaactgc ggtcgtgctg agcggatcgg ggggcatcac cacggttcgg ccttcaatca
     9241 aagagaagaa cttttcctgt ccggaaggcg tatcgcttcg cccgctatag taccttggag
     9301 ccttcccctc ctcgttgagg aggcacacta cgtaccgtcg gtcgacaata tcgactcccg
     9361 ccactggctt cttgttgtca ctcatggttc aagcgtaccc ggggaaagac cccaaaccca
     9421 gttgtaaaaa tccgctcttc ttttcttttt cttcgcttct tagtattctt acggatagga
     9481 ggaaaccgat gaaacagaat gaacgactct tggtcgtagt ggatatgcag aacgatttcg
     9541 ttaccggctc gcttggaacg gaagaagcaa aacggatcat cgatggagcg gtggcgaaga
     9601 tcaacgcgga aaaaggatcg gtcgcctaca cccttgacac ccactttgac gattacctct
     9661 ccaccagtga aggtcgttcc cttcccgtcg agcactgcat caaaggcagt gagggacata
     9721 agctgatccc tgagcttcga agccccctca aagatgccaa gagctttgaa aagcacacct
     9781 tcggctccgt tgaacttgcc gagtacattc gagacaatcc ccagatcagg gaagttgaac
     9841 tcttcggtct ttgcaccgac atctgtgtcg tctccaatgc gctgatgatc aaggcgatga
     9901 gaccggaact cgtcgttaag gtagacggac gaatctccgc cggaaccacc gttgagaaac
     9961 acaacgccgc tttggacacg atggagtcct gtcaaatcgt ggtaacacgg taattataaa
    10021 ccttttcata gacaagtttc tctcctccga gtaggatagt gtataccctt ttggaggaga
    10081 ttttgatgct gcaaccccta tcatatgttc ttgtcacccc ctacaccgtt gcgaaaagca
    10141 ggaccggtgg tgtcttgtcc cgcttgctat ctcgaatttc acttgaactg gtcggcgccc
    10201 aaatggttgc cttggacaaa gagactaccg aagcgttcgc ccaaattata gaaaatcgaa
    10261 atcttgcagc gtgttgcggt gcaacgcggg acatcctcgg ttcctatatt cgacaaaact
    10321 tgggcccatc cgatgacggt tcgctccacc gttccctctt tctcgtcttc cgcggtgaca
    10381 acccgacgaa ggagctcagc accgtctgcg gcaccttcca gaaagacgct gacgaccttg
    10441 aagccgtcac cggcgaatcg attcgggaca cctatgcgga tctgatctat accgacgaaa
    10501 gccaacagac gctccgttac ttcgagccgg cggtcatcac cgccagtgag caaaaggaag
    10561 cggaagcggt catccggctg tttgccaaat ggcttccgac ccaaaacaac ttgattcaca
    10621 atcgttccga ggagtactac aagggcgttg agcgaacctt ggtgatcatc aagccggata
    10681 actggcggta cgcttcctcg cgaccgggca tgatcatcga catgttcagc cgcagcggat
    10741 tgagaattgt cgcaatcaag gtcttgaaga tgagcgtcgc ccaggcggtc cggttttacg
    10801 ggccgaccaa agaaggactc aaaaagcgac tcgccccgat ctacgggatg caggctcgcg
    10861 agcttcttga gcgggagttc aatattcccc tgacggaaga gcttgagaag accctgacgg
    10921 aatcattcgg cgacatgtac ggtgaagagc agtttgaacg gattatcgag ttcatggccg
    10981 gaatcaagac gtatgagcgg cctgaagagg aatgggaaga accgggcttg gtaaaatcga
    11041 tgattctcgt ctacgaagga aaggatgcga tcagcaagat tcgaacgatc ttgggagcga
    11101 ccgacccgac caaagcagcc gccggaacga tccgacggga gttcggttca aatatctgca
    11161 tcaatgcggc ccacgcttcc gactccgtcg agagcgccgt cagggagatg ggaatcctcg
    11221 aagtggaaaa aaaccacctc gggggagttc tccaacacta cgtggcacat cgctgagtta
    11281 tccccgttca aatgcgccca catcatcagt caagccgttc tccacttgtt catcgtatag
    11341 gaaaccaagt acagtaggat catgaccata gcgccattcc tccacatgct tcccatcctc
    11401 gtcgtgatcc tctcagggta tgtgatgggc cgcttgctgc cgatcgattc aaaaacgctt
    11461 gccaccgtgg ctgcggattt cttcatgccc gcccttctgt tcatatcgat ggcgacgacg
    11521 gatctggaag gttcactcgt ttcatccctg atcggctcat cgatctccat caccgcaatg
    11581 ctcttgatcg ttgcgttcat ctgggcgaag ctcgccgggc aggagccctc gaaaaccgtt
    11641 ccttccctgg tcttcatgaa ctccggcttt cttgcaatcc ccctgatgaa gctctatggt
    11701 ggaaaccttg ggatgaatat gagcgtgatc ttcgacttca cccaatcgtt tgcgatgttc
    11761 acggtcgggg tgttcatcat caccggcgga ttgaaggctt cttcggtcaa ggcggcgttt
    11821 gcctccccaa tcatctgggc gaccgtctcg ggtcttctgg ttcgcaccct cgccattccg
    11881 atacccgaga caatccgaga gatcttcctc tatgccggcg acggtgcgcc ggctcttgca
    11941 agcttcgccc ttggcgtctc cctgaaagac ttcaagctca ccttgaaagt cgagttgatc
    12001 agcgcattgt tcgttcgctt tgtcggaggt tttttgatcg gactgttggc ggcgaacctc
    12061 ttcggtcttc agggaatggc caaagtcgtc gtgacggtaa ctgctgccct tccctcggcg
    12121 atcttcacca gcgttctgcc gatccgccac aacaaggacg cgaactttgc caccacgatg
    12181 gttctcctct ccactcttct gggagccttc acgatccccc tttcgtttgc cctgatggga
    12241 taaggaaaga gttccctccg gggacaaatg tccaaacacc acccataatc ggcttgttta
    12301 cccccggtgt gtaagctaca ctgggtggta acgtgcagag gagggactat gcttacacgg
    12361 cgagtaaagt ttggttttgg catcggtgac ttggggggca accttttctt cacgattatc
    12421 ggtttctatc ttctcttcta tttcaccgat atcgtcggcc ttaatcccgc cttgagcgga
    12481 accgcagtga tgatcggaaa agtgtgggat gcgatcaccg acccgatcac cggctactac
    12541 tccgatcgaa ccaagagccg cttcggccga agaagaccgt atatcttcat cggggcgatc
    12601 ttctcctttt tcttcatgat ccagctcttc accccggtct ccatgaagaa cgagatgagc
    12661 ctcttcctct actacacctt ggtctactcc ctgttgaaca ccgcctacac gctggtaaac
    12721 atcccctaca cggcgctgct gccggagctc accgacgact atcacgagcg gacgattctc
    12781 accgggtatc ggatgagctt tgccgtcttg ggcaccttcg ccggagcggc gttggtgatg
    12841 ccgatcctga acgccacctc ctggccggtg atgggcgggg tgatgggggg aatcatgctg
    12901 gcgtcggcga tgattaccgt ctttgcgatc cgggagccga aggagcattc caagcccggc
    12961 gaggacggat ttctttccac cttcgccgaa gtgctcaagg aaaaggtgtt tcttcaagcc
    13021 cttctgccgt ggacgttctt catcaccggt acgatgatga tccagggagg cctcgtctac
    13081 tacttcacct atatctacgg caacaaggat ctgttccagc ttgccctcgt cggcctgctc
    13141 tccttcagcc tgctctgcat ccccttttgg gtatccgtct ccaaaaaaat cggcaagaga
    13201 aaagtgtata tgatcggcat gtcgatcgtg agcgttggcg tcctgctctt caccaccctt
    13261 ggagccgtgt tcggaccgat cttcggcatc gtggtgatga gcctggcggg gatcggtctt
    13321 tcgacccact acgtgatgcc ccattcgatc ctgccggatg tcgtcgagta tgatgcccat
    13381 accaataaaa aaggtcgccg ggaaggagtc ttttcaagta tctggacgtt cagcagcaag
    13441 atcggccaag cgttcgccct tgccctcaac ggctggatct tagcgatctt cggctaccag
    13501 ggaggatcgt tcctgcctca aacaaaaccc ggcatcatcc tcattgtcgg tccgcttccg
    13561 attctttggt acatcctcgg cctcctgatt ctaaggcact acccgatcga ccaggcctac
    13621 tacaatgaga tgatcaagcg tggcgaataa ggatcggttt ccggcacccg catactcgcc
    13681 gctcgcccgg atgatcgtac cgctgttcgg gaagctgtat ctcaggctcg ccttgggggt
    13741 atcgacgatt gtgcttcccg agggggaaat tcttcgcaat gagctgcggc ggttcgaaga
    13801 aagaagatca tccctcatcc ttgccttccg ccataccgcg gtcgaggacg ctcccacgct
    13861 tctcgtcggc gtgaaacacc ctcatctccg gttcctctat gggcgggatg tcgtcaactg
    13921 ggcaggttgg ccaaccaaga tcctctttcc cgccctcggc ttcatcgccg tggagaaccg
    13981 ggggaacaac cgggaaggcg cccaatatct ccgcaaagag gtgcaaaacc cccgtttccc
    14041 gatcgccctg gctccagaag gccaggtaac ctatcacgca tacacatgcg cctcgatcga
    14101 gatgggagcg gcgaacatcg cctcgtgggc tcttgagggt ccgcgggagg aggtcgtcat
    14161 tcttccgatc ggcatcggat accgatatgc gaaagacaac gaccgtttcc tccttgatct
    14221 catcacccgc tgggagaagg aagcgaacgt cgcagtcgat cggagtcttc caccgaatga
    14281 acaaatccgc gccatcggct atgagaccct gaagctggtc aacaccttct ggaacctgaa
    14341 tctgaccttg aaaggctcct tcatcgaaca gcgggacggg ttgtgtgatg ccctgctccg
    14401 ttactcggag tcgctgggcg gtctggaaga ctcaagcggc tcgatcatcg accggctctt
    14461 cagagtccga tacaaggcgg tgagcgtcct gaacgacacc gaccgatcct tgttgagcga
    14521 ggatgaacgg cgtcttcatg atgaagaggt aacaaagcag aagatctccg accatgccgc
    14581 tcaggtggtg gatgtgcttg aatatatcga tttgaactat ttggaaggga aacatgccgt
    14641 tcagcgttcg attgaggtga tgctctccct tcttgatgtg ctgaatcggc tccaaggtgg
    14701 aatgatcaat tcgcgcttca gccccaagag caaaaaggcg ttcatccttg gcggcactcc
    14761 gattgaagtt cgaaaaagct tcgggcacat caccgggaga aaagagcggc tgaatgcaat
    14821 caatcaggcc cttgaggagg gcttgaacaa cgtgagcctc acccttgaag agataatgtt
    14881 tcagtcaacg taaaggttgg atcgaaggtt ttgtaatatc cctgatggcg ctcatccatc
    14941 aacgaggcga tgcggaccat catctcatcg gcaagagcct gccgtccttc ggcatccagg
    15001 cgtccccctt gggcaatcgg ccggaacacc tcacccacct tgatcgtgat ccttgtccgc
    15061 cgcaatcgca gaaggttccg tctgaagttt tcaaagccgg ttacagcgat cggaaccatc
    15121 ggagtccggt tcttataggc gatgaaggcg attccgctct ttccccgctg gagggtgccg
    15181 tccttgcttc gggttccttc gggagcgatc gcgaagatct cgcccttgtc aagcaccgta
    15241 aagatcgcat ccatcgcttg gcggtcgaca tttccccgat cgaccggaat cgattcccaa
    15301 aggttcatca gataccgggt gacgggattc ttccaaagct ccttcttggc aagggcgtgc
    15361 atcgctcggg gttgaaggta cgcatagagc atcggccctt cgaagaccga ggtatggttg
    15421 acgatcatca tcaagggacc ttcgagagga accttcacca attcactgcg atctatcttg
    15481 aaaaagagtc tgaaataggt tctcaacacg ctgttgacga aacgcttggc aaaactcatg
    15541 gctcatccta caaaagagga tgaaaagaaa caagctttaa tgagcgcttt tcagccccgc
    15601 cccggtaagg ggaatcaggg atgttttatc agccagcatt ggatcttcct tcagggcgac
    15661 ccggtagccg gcgtagttgg ccgctccggt gtactccaca tatacgccct tcttcgcaag
    15721 gtcgccatgc gccttgagga tttcttcctc cgttaccgta acgaatcgcc cacccaggtc
    15781 atgaaccgct tgcaggatct cctttccacg agcgggctct cccgtcgcga ttccctcggc
    15841 cagcgtcggt gaaaccaaga ctgattgagg ggaaagggat ccttgcccat aggcttgggc
    15901 cagaggggcg cagccggccg cctggagggc gatgacaagc ggatgggaat cgatcaggtt
    15961 ccatgcttta agctcttgca acgcaagata aacacccatc agaagggtcc cgttgccgac
    16021 cggcacgatg agaacctccg gcaacgaccc gaaccgttcg tagagctcgt agacgtaggt
    16081 cttcgttccc tcccaaaaga atggattgaa gatgtggctg gcgtaaaaga tttcggattt
    16141 gtccacgtag tcgatcgtcg cccgggcggt ttcatcccgc gaaccttcaa tggcatggat
    16201 cctcccgcca tgcgcttcga tctgggtcag tttcttagcg ctggtcgaag ccgggacgaa
    16261 gacttccgtg gcaatcccgg cccgggcggc ataggcggca acggcggttg cagcattgcc
    16321 gctggagtcg atcgcacagc gcttcacccc gatttccacc attagcgcgg cgagcatcac
    16381 cgccccccga tccttgaacg agagtgttgg catcagatag tccgctttgc cgtagaggtg
    16441 ttcctccaat cggataaccg gagtcccgcc ctcgcccatc gtgacacgct tgatcgtctt
    16501 ttcttcaaac ggcggcaagg ccgaccgata gcgccacaga aagcggtcgc ctcccaaaga
    16561 gccgaaggcc ggagcggtct tgggatacga gaggtggaac agcgaaccgc attcgcaggc
    16621 atatctcttt gttgaaagcg gataggtttt gccgcactgc ttgcagatat acgtgatcat
    16681 tcgacttcat ccttcagctt ggcgggctcg gtatgcagga tcgccgagcg ggcgagcacc
    16741 acggtggggt cgataagcgg cagcgaaatc gtctcccttg tgagcgccag cggcagttcc
    16801 gtgcacccga gaatcagggc ttcggctccc tgcttgtgca gcttgtacgc ctcttcgatc
    16861 aggatcgtca gcgcttcccg gctgaccggc gcccgggcct tgattccata ccgttgattg
    16921 taaatcgcat cgtgtacccg ctccctgctc tccctgtcag gttcgatcag agtgatgtgc
    16981 ggatatgcaa ggagggcgtt tggataggtt tttaaggcat gggtgccctt tgtcgcaagc
    17041 aagccgatcg ttgtgggctc gggaaaccgc ttggctatgt gcttggccgt ctcttcgatc
    17101 atgtgcagca aggtgatgtc gggatgggaa tccctgagct tcttcctaag cggatcgaag
    17161 atcggaggag catgggcggt gttgcaggga acgatcaggg ttgaagcgcc cagaccgatc
    17221 aattgaaggg cgtttttgta caacccttca gcggggttgc ttcccccttc gagaagaaac
    17281 ttggtgcggt ctccgatcga tctcggcgta gaggtgaggt acaggtcgat gtgctcttgg
    17341 tccgttgcgg cattggtatt ctcaaaaatc ttttgcgcgg catccgcgcc cgccatgggg
    17401 cctacccccc cgatgattcc gattgcgttc atgtatacag agtaccgcac catttcctcc
    17461 gctacaagaa gtgttgtatg ctatagtgga ttttggagga aggaagaatg gcttcaatca
    17521 gatcgattcg ggaacggatg caggaactct acgtctatga cctctatggg tttcgaagcg
    17581 aaaacaaggc gttggccggt gttctctatc cccatgaaaa gatcaacagc ttcgccgtcg
    17641 gcctccatga agggctgaag aagatgctcg tggttaccac ccaccgcctc atcatcatcc
    17701 gatcctcgtt tggaaaaacc cccgatgtcc aggcggtcaa gcgcgaactg atcgaagaac
    17761 cgtcctacac caagcggttc ttcaattcgg agctttcttt caaactgcac ggcgaaactt
    17821 tcacctttac catggtctcg cgccgagtcc tggagctctt tgtatgggcg gttgaacaac
    17881 ccttggaata aacaaagggg gcccgaaggc cccacattgg tattgcattg ggtaatggat
    17941 tattcccaag ctttctggtc caacagaccc atgtttttgg aaacgaccgt cgtacagacc
    18001 aagtctccgg ttacgttcag ggcggttcga cccatgtcga ggatcgcatc gattccaaga
    18061 atcatcgcat atgcgccggc aacggcggat ccttcgacga ccggaagtcc gacggattcg
    18121 agaaccatca gaagcatgat cgcaccggct ccgggaacac cggccgttcc gatcgatgcg
    18181 agggtcgccg ttacgatgat catacccatc tgacccaagg tgaagttggc gccggttacc
    18241 gtcaagctga tgaagatcgc gcagacacct tgatagatcg ccgttccgtc catgttgatc
    18301 gtcgctccga ggggaaggga gaacgaatat acatctctgg gaattccaag tctctttgcc
    18361 gcttcaatcg ttaccggcag cgttccgttg gaagaacggg taacgaatgc ggtgatgaag
    18421 ggatcctttg catcattgaa aaatcgtttg atcgaaagtt tgttgaccat caacattccg
    18481 ccgtagacga gcacaacgtg aatcgcatat ccaaggaaac atgcgatcgt cacgaccgcc
    18541 aatgatccgg ccactttgga gccctgcttc gcaaatactt cggcaacaag agccaatact
    18601 ccgatcggag catactgcat gattcccgtt accatcctca tcatgacttc cgcggtgccg
    18661 tcaaagacga cgatcagcgt ttcacctgcg gtcttcaagc gcttatcctt gctgatgttc
    18721 agataggaga tgccgatgcc gaagatgatc gcaaagaaga tcacctgaag caccgtttcg
    18781 ttcacgatcg cttgagcaac actcgtagga atgatattga ggagcaccgt tgaaacggga
    18841 gtcttcgccg cggcttttcc tgctgcaccg gctacaagag cgcccatttc cgcactcggc
    18901 cggaagatgg aacccatgac gaggccgaca aagaccgcaa atgctgaagt aacgaggtag
    18961 aagaggacga ctctgagacc taccttaccc agtttcgccg ggctgatgga tgcggctccg
    19021 acgaccagtg tggagaagat gatcggcatc atgatcatct tcagcagtcg gacaaagaga
    19081 ccaccaaggg gagcgatcca gagcatcttc tcaccaagaa tgattccaag caacgcacca
    19141 agtacaaggc caatcaggat tcgatagagc aggttgaagt tgaaatacca acttaacaca
    19201 ccctttttga ccggggcctc ctctttcttg accggagcct cctttttgac ctctggttct
    19261 tttttcactt cagcttcttt tttgactgga gttttcttct tttcgtctgc catactaacc
    19321 tcctgaggaa catgccagtt tgtcgctcaa caattgagct tctctaatca taacattaca
    19381 ctaccaacgg ttagaatgaa acaaaaatct ttgattat
//
LOCUS       DAOSLI010000034        18648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3207_length_18648_cov_25.014199, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000034 DAOSLI010000000
VERSION     DAOSLI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 18648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18648
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3207_length_18648_cov_25.014199"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLF67_06125"
     CDS             <1..519
                     /locus_tag="PLF67_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOE89625.1"
                     /translation="RKAKESRIYELSQVGEVPIEVSYQIPFRHLCNLLQLHEGDIDRA
                     LATLEDATPGQLERLKTRAICAWNWITTFSPEDFRFRLMKDSDSKVEIEGAELAAIHA
                     LREVVKEMNTLDEQTYTTRLYDAAKDNGLDTGDFFKLVYRVLIGRSHGPKLASFLETI
                     GREKALEILSRY"
     gene            521..1135
                     /locus_tag="PLF67_06130"
     CDS             521..1135
                     /locus_tag="PLF67_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOE89626.1"
                     /translation="MKVLMINLGPHQKGATNRALVEVAKGLDEYKVEHEILWVGTDPI
                     QGCIDCKACFKNRRCVFDDLVNTAAEKVRASDGLILGSPVHYASAANTLFVDRLFRVV
                     SKEVALKVGSSVVSCRRGGASATFDQLNKYFTISQMLVVSSAYWNSVHGNNASEVEQD
                     HEGLQVMRILGRNMGYVINRLHGRDVEKPLPEQRVWTNFISSER"
     gene            complement(1453..1842)
                     /locus_tag="PLF67_06135"
     CDS             complement(1453..1842)
                     /locus_tag="PLF67_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HOE89627.1"
                     /translation="MLAILCSLTVAAVILEGPDNLPGKLFYLQTRSALLNVTSVGFLG
                     LVLMYFAAINLSGPTLATIMTMMDFSFFGNTLYNSVPVIIGILAGTIYVVVVDVTASW
                     HGGLALYNNGFAGGLTPSLIISVILWF"
     gene            2032..3225
                     /locus_tag="PLF67_06140"
     CDS             2032..3225
                     /locus_tag="PLF67_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89628.1"
                     /translation="MKRIARLIGVVFIILLLSIALVSCDDALLQHVPQNGTQNEAQNG
                     TQNNDQNEEQDEAEEEDEESEDEGDESGDEGDESGDEDDEFDGLPRKGDFTYIGTPPT
                     NHAAIVEASNEIKEEMERIKRELDHARENTETIDDISVELFKMMGNAFKNFVVDYEND
                     GYAGMEVFSVLLEGVDVESVDENALLQVDADSVKKSMFTRILFDESDSKSMETKVGMS
                     AEFEIVGSEMGIDGEYKIKLLMNVEGDDEEQEVTLVGTLTNIAEDTEDTETPLTEADL
                     LLGDDEWYEWDQEGAYPHQRTLTLFMEIVSQFDMLSETLATQIFYPEITGDNGTGKFI
                     GLVSFYQEGDAAEEEIPLYLAPMYLSFLWEGADHEKYYVVYSSHEDYQTIVAINGVAY
                     NFALP"
     gene            3490..4596
                     /locus_tag="PLF67_06145"
     CDS             3490..4596
                     /locus_tag="PLF67_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89629.1"
                     /translation="MKKVVRMLGGAFVLLIMVFMVITLVSCDDDLLPNGEHNGFPDLP
                     DNGFPDPPDGWHAEWPDDWKNEWPGDWPGELPEDFSFELPEALPSTCDGTRALIQSVE
                     DAIVIIKNDLNTAKGNTEINDLPALDEGSIQYKAKTAFDGLLANRNPKHPGITLFQFG
                     LYGDSTKENISGVTIDFDSPEGCFMDMMNEQGAFINIELDSKFTINEAEGVPEELPGS
                     YEISLKGALMEKAPGTDVIVIGSTLEKTGEGAWNKNLTGTDVKFDDEGDYEWNQAGAE
                     PHHKILTLLMSSLGGAKRKEFARMAFYFDVTFEGVEGKFIAGITAFGAERKSYSSVIW
                     KDNDNNEHLIVWSNHKDYEDFIVIDKVAYKLPKT"
     gene            4836..5774
                     /locus_tag="PLF67_06150"
     CDS             4836..5774
                     /locus_tag="PLF67_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89630.1"
                     /translation="MKKIVRMLGGALVLLVMIIALVSCDDNVAPNDVSFDFTKTLPKT
                     RSEAEAAIDSIEKDMNAIKDALDNAKKGTLAPYKEEFNDLIGDLPGLNPYMSGTESYW
                     FGLYSDSSEINHYGITDAPVTSATGFFADKTKGAHSDITIIVDTKFKINAVMEGKPDI
                     RGSYEISLNGSYVEDAGIIVPIASGTLKKTDVPTWSKSLTTADVLLDEGKYKWDKNKG
                     ENEDNYEPHQRILTVFMSLSLTSEKAAMKVFYTSAKDEGVKGEFVSGYTVHGEGGSWT
                     SATWKKGDKQYTFLYSDTEDYDDVIIINEVAYTVVT"
     gene            6400..6909
                     /locus_tag="PLF67_06155"
     CDS             6400..6909
                     /locus_tag="PLF67_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89631.1"
                     /translation="MRKLIILFVFLVVGFQPVSAGTPTFHAGVSGGVGFFSWYTSLEA
                     GIKVDLRENFALGLTQRVGYGFTYREIVGGTELRAYMYDDLFIHIGVSYLLKPSSCAE
                     PDFKTMVLPHLGFGLYIPIDSGRRFFVVPRVEMNQSFYLSDSIRPVYSDLPFLVAGQA
                     SIGFEYRMR"
     gene            complement(7019..8053)
                     /locus_tag="PLF67_06160"
     CDS             complement(7019..8053)
                     /locus_tag="PLF67_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOE89632.1"
                     /translation="MDAGITDFQELIQKFSELPNQPREDENLFSICGFPHYERVISNV
                     LAFFLDNQREHGLGNLFIDTLLSFCGHKDLDLNYRVETETPTDKGKFIDIDIIGDEYR
                     IIIENKINASLYNDLNEYFKFASKNGRTVIAFVLSLKPLDPGINKKYTFITYEKLFKE
                     LRRRMGEYVVGANQNYLSLFFDLVKNIDNLQGRDSMDYEFIKFVKENQDAVNLMNEKI
                     KSLQYEMRKTVKSVNSLVEDAISSKKIRQWPWRVLPYLEDTAVTDIDFGNVGIAIDSI
                     LNLTGWHFGVFIRRANEEFDLETFLKNRSIEYQYKEGKKYFLNQTYGIEESPTVVAEY
                     IIKVITALIG"
     gene            complement(8175..9056)
                     /gene="era"
                     /locus_tag="PLF67_06165"
     CDS             complement(8175..9056)
                     /gene="era"
                     /locus_tag="PLF67_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOE89633.1"
                     /translation="MKSATVAVVGRPSAGKSTLVNTICEMKVSITAKTPQTTRNAIKG
                     IYTDQRGQLIFIDTPGYHLGTRVLNLRLQETAVKSLSESDLVLYLIDGKRSAKEEELS
                     LARTLAKLKTPVICLINKADILTEEEIEETKAFLKEHLPSSPILIGSARNDEGIDEVL
                     IELFKVAPEGPLLYDEDAYTDQELEFRIAELIREKAIALVTEELPHAIYVDVADIEYK
                     EETNTIWIRAFIVVERESQKGIVIGKGGENMTKIRKAVEPEIRSIFPKRKLRLDLKVK
                     ANPRWRTKPHILDNLLR"
     gene            9172..9741
                     /locus_tag="PLF67_06170"
     CDS             9172..9741
                     /locus_tag="PLF67_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOE89634.1"
                     /translation="MAEEKKKTQSEAQLQEKLLKTRSVLLSGEINKESADEVIRQLLV
                     LEGESNEPIRLYINSPGGDVDAGYAIFDMVRFINAPVTMIGMGLVASAAVLVLLAVPS
                     ERRIALPNSTYLIHQPMSGMRGVATDIEIHAQYLERLRSKLDSLIASETGKSVEEVAN
                     DTDRDHWLTSEEALSYGLVSRIVAERSAL"
     gene            9748..11967
                     /locus_tag="PLF67_06175"
     CDS             9748..11967
                     /locus_tag="PLF67_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOE89635.1"
                     /translation="MPKTKYLSDKEKRIGQKHMYRQEVFNGISFSLMGETVVYLLALY
                     FGAGNFALGYISSVIFLAGIILPVVPRMLKGKHHTRTQSFVWHFRGLVGLGYLGLFIV
                     SGDWAVFLLLTVYTLYNLVRMIGIALNDSTMKSISSPSNRGKVVANANVAYQSSSMTV
                     RLIMTAVFAVQRFSGLVGLVTMQVLGVVANCFASREIKKIPSRMVITHKPGRTVWTLF
                     TEAMAQPVLRRRLLLKWLATGVTVIFGLTTPFFRIELGSSNGIVVLYSVLLGLSVMVA
                     SWVSKRFSDRLGSKPLVVISTLFTLGFFALWAILPRTLHFAWFFGLGFLTNTFVALIN
                     LLTFRLLTQVMPDDELVSFNSMVNFINGIVAFGVGMLSGFLANFTQGSLLFHGTALGN
                     GYTLVFIFGFALILVEALVALRIQEIGAYSSQAAAQVVFSMHGIRAVSMIERLERTSD
                     PAKRRMLMHNLGGNVNYLATSELRSILASPFHVDKLEAVRAIGDRPRKSLLDDLIRVA
                     QDDDSYVQLDAIAALGSYRKEEKAKSALVDLLLHGRWASVRSMASKSLARISDSDEYL
                     NVVNELSRSAKHIDEVIDYLVAKRFMDKEGRFFQEFFIFVEQGRSGTFRQTGYSVVAS
                     LLRFGPPSLASLYEERNLSSTKAYLAGFLSEARDLTMIDQNYNLIIQIFAKEQWSKLI
                     DLCLESLRSSDVQADSSLDNLKKGLLKVETMSLESFDVVDMIALLYFTYFICKSQNS"
     gene            12000..13010
                     /locus_tag="PLF67_06180"
     CDS             12000..13010
                     /locus_tag="PLF67_06180"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89636.1"
                     /translation="MIVLLLILITSCFGCSCDIPADEHVFRVLSYNVENLFDATLDGT
                     EHAEYQDPGKWSERSYRLRLRNLGKILLDPALNHPDVIILQEIENERVVEDLLSFHLA
                     KKGYRSYAVARTEGSPISVGAISRHPIVRSSVHAVPGGRNILEVVISTPVEEVVIFAL
                     HAKSQIGGFSESELLRIENAKSVSLASRDHVDKAVLVCGDFNQNPRVVHEHEGVQTAL
                     VDVIHPNAKLYGRAGSLLISGNADFLGPGIFYNPYLAEDCRLAGSYCYGGVWHRYDQI
                     LLSPSLFDRRNWEYSTFEVIRFPYLLKSDGTPKRWDLKTLSGYSDHLPVMVTLTRSPW
                     EI"
     gene            complement(12985..13623)
                     /locus_tag="PLF67_06185"
     CDS             complement(12985..13623)
                     /locus_tag="PLF67_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOE89637.1"
                     /translation="MNNDQLVRVTRYFRTLNRLKTIGLERVFAHNLADAAGVSAATVR
                     KDFSQLGIYGQKRGGYALSELLASLAELLGKGDSQNCIILGCGRIGKALMHYSGFEQD
                     GIRVVAGFDSDPLVYSDSSHPIPIYPLSRIDEVVKALDVKVGILAVPEQAANDSYERL
                     LGSGVMGILNFTPITIKAQARKDGRMPVVHNINIALELEQIFYELKFPRDSE"
     gene            13800..14273
                     /locus_tag="PLF67_06190"
     CDS             13800..14273
                     /locus_tag="PLF67_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE89638.1"
                     /translation="MTETNTVTFSPELVNFIKTWKDKPGNLIMVLHQVQEEHGYISRS
                     AADQVAAMLDVPLATIWGVVSFYHFFKLTKPGKHNIQVCMGTACYLKGGQPLIDELSK
                     QLKIGVGEVTEDGNFSLQAVRCVGCCGLAPVLTVGAEVFGKVTKDEIASIIKQFS"
     gene            14284..14823
                     /locus_tag="PLF67_06195"
     CDS             14284..14823
                     /locus_tag="PLF67_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOE89639.1"
                     /translation="MHIAITDYVLEVVQNAFEANSTNTRLMLQETAEAISVTVADNGK
                     GMSEEVMKRALDPFSTEEGKHPLRKVGLGLSFLSQATELAGGKLTLQSEVGVGTTVHF
                     TFDPASIDTPPLGDVVSTFMTLLAHPKAESLVIERKTETDGYALDRQELIDVLGDLEM
                     SGSLHLLKEYIASQEAALS"
     gene            14831..15235
                     /locus_tag="PLF67_06200"
     CDS             14831..15235
                     /locus_tag="PLF67_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOE89640.1"
                     /translation="MAKMTLEELRRLREEKQEALEKRSIEGKDFRIIIGMGTCGIAAG
                     AKETLAAFLSVLDEEKIKHVAITQTGCMGLCHVEPTVEVILPDMPPVIYGNVDADVAR
                     RIVAEHILGKRLVTDHMFDRPAIDIITQGGGR"
     gene            15235..>15617
                     /locus_tag="PLF67_06205"
     CDS             15235..>15617
                     /locus_tag="PLF67_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOE89641.1"
                     /translation="MAFKHYILVCGGTACESSKADAIYQNLIKECEANGIVDEVQIVK
                     TGCFGFCEQGPIVKILPEDSFYVKVEPSDAKELIAEHIIKGRQVERLLYDKDLSKKHA
                     KVEDIQFYQKQFRVVLRNCGTIDPE"
     assembly_gap    15618..15717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15718..16776
                     /locus_tag="PLF67_06210"
     CDS             <15718..16776
                     /locus_tag="PLF67_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOE89642.1"
                     /translation="RLEIAMAQAREYGLLGQNILGSGYEFDIEIRLGAGAFVCGEETA
                     LLRSIEGKRGMPTPRPPFPAVKGLWNRPTVINNVETWANIPVIISKGGNWFNRIGTAD
                     SKGTKVFALTGKIRNSGLVEVPMGTTLREIIYDIGGGIPNNKKFKAVQTGGPSGGVIT
                     EDYLDTPIDFGSLVKAGSMMGSGGMIVMDEDDCIVDVSKFYLDFTVEESCGKCAPCRI
                     GGRSLLNILDKITLGNGTMEDLNTLEKIGEAMRKGSLCALGQTAPNPVASTLAHFRDE
                     YIAHIVDKRCPSGTCKNLVTYSINPEKCTGCTLCARKCPVNAITGERREPHVIDPSVC
                     IKCGVCHDVCRFGAVLVS"
     gene            16788..18536
                     /locus_tag="PLF67_06215"
     CDS             16788..18536
                     /locus_tag="PLF67_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOE89643.1"
                     /translation="MSDFQVKINGISVDVTRGMTVLQAATQAGVKIPTLCYHDDLVPF
                     GSCGLCIVKQEGSSKIIRACSAPVVEGLSVITHDTELFEVRKTILELILSTHPSTCLS
                     CIRNGECELQDLAAEFGIREQPFEVRLSDRPKDTSTASIVFEPEKCIKCGRCVQVCQD
                     LQGVYALEFIGRGDMTVMAPAAMMKLEDSPCVRCGQCVVHCPVGAIYEKDQIADYIKA
                     MNDPEKQIVVQIAPAIRVGLSESFGLPVGTVTTKKIYAAIRRLGAVAVHDTNFGADLT
                     IMEEGSELLGRMSDGGLLPQLTSCCPAWIDYVQKYYPDLLEHVSSAKSPMQMVGAIQK
                     TYWAEKMGIDPAKLFTVAIMPCTAKKHETIRDKAMFSSGHQDVDLVLTTREFARLIKR
                     VGIDFLSLQEEEADNPIAEYSGAGTIFGATGGVMEAAIRTAYNVVTGKELENIDVEVV
                     RGLEEIKKGTVDFDGTPVRVAVIHGLSHAKEVMDEIRAAKAKGERAPYEFVEVMACRG
                     GCIAGGGQPYGATDILRKERAEGLYQDDRQSAIRCSHQNEAILKLYEEYFEKPLSDKA
                     HHLLHTTYQEIPVYKA"
BASE COUNT         4427 a         4574 c         5155 g         4392 t
ORIGIN      
        1 cgcaaggcga aagaaagccg gatctacgag ctcagccaag tgggggaggt tcccatcgag
       61 gtaagctacc agattccgtt ccgtcacctg tgcaacctcc tccagcttca tgagggggat
      121 atcgatcggg ctcttgccac attggaggat gccacccccg gccagctgga acgcttgaag
      181 acccgggcga tctgcgcctg gaactggatc accacgttca gcccggagga tttccgcttc
      241 cgcctgatga aggacagcga ttccaaagtg gagatcgagg gcgccgaact tgcggcaatc
      301 cacgccttgc gggaggtcgt gaaggaaatg aatacgctgg atgagcagac ctataccacc
      361 cgcctctatg atgcggcgaa ggacaacggt cttgataccg gtgatttctt caagctcgtc
      421 taccgggttc tcatcggcag gtcccacgga ccgaagctcg cttcgttcct cgagacgatc
      481 ggtcgggaga aagcgctgga gattctcagc aggtactgat atgaaggtgt tgatgatcaa
      541 cttggggcct caccaaaagg gcgctaccaa ccgcgctctg gttgaggtcg ccaaagggct
      601 tgatgaatac aaggtcgagc atgagattct ttgggtggga accgatccta tccaagggtg
      661 catcgattgc aaagcctgtt tcaagaaccg ccgctgcgtg tttgacgacc tggtcaacac
      721 ggcggcggaa aaggtccgtg cttccgatgg tttgatttta ggttcgccgg tgcattacgc
      781 ctcggccgcc aacaccctct tcgtcgaccg tcttttccgg gtcgtatcca aggaggtcgc
      841 cctgaaagtc ggctcttcgg tggtctcgtg ccgccggggc ggagcgagcg ccacgttcga
      901 tcagttgaac aaatatttca cgatcagcca gatgctggtc gtctcctcgg catattggaa
      961 cagcgtccac gggaacaatg cgtcggaagt ggagcaggat catgaggggc ttcaggtgat
     1021 gcggattctc ggacggaaca tgggatatgt gatcaaccgt ctccatggaa gagacgtgga
     1081 aaaaccgctt cctgaacagc gggtgtggac gaacttcatt tcttcggaac gatgaaggaa
     1141 tcgctgccca gccatagatc gatgtggatg ccgggattgt tgttcggcac agtcgcctgc
     1201 ttgatctgcc agccgatcag tttcagcctg aatcacaaat tcaattccag ccgatttcgt
     1261 tttgggttga gggtggtccg aatcttccgg tcattgaaga aggagagatg ggccccgtcc
     1321 tcttcctagt ggagtgaaac gaccagacga agaggatcgc cttccggtag gaaatcagtc
     1381 atttcgccga ttaaacagct gagaaattct tttcgtttta tcaggtgaaa ttctcccgta
     1441 tcctgttcgc gttcaaaacc aaaggatgac tgaaatgatc aggcttggcg tcaatccccc
     1501 tgcaaatcca ttattgtaca gagccagccc tccatgccaa gaggcggtaa catcgacgac
     1561 gaccacatag attgttccgg cgaggatgcc gatgatgacg ggaaccgagt tgtagagggt
     1621 gttgccgaag aaagagaagt ccatcatcgt catgatcgtt gccagcgtcg ggccggagag
     1681 gttgatcgcg gcgaagtaca tcaggacaag acccaggaag ccgacgcttg tcacgttcag
     1741 gagggcgctc cgggtttgaa gataaaacag tttccctgga agattatccg ggccttcgag
     1801 gatcaccgca gccacggtga gcgagcacag gatggccagc atcagcagat aaagagttca
     1861 ttcgctttta aacaaaaaca acctccttgc acccgaccga tgagaaacgg tatggagtac
     1921 attcgattgg taagacattc aagcgtaatg aaatatgttg aaaaaaatag acatccggga
     1981 tcgaccatgt tataaaagaa aaagaagaaa taacgttgta aggagagata gatgaaaaga
     2041 atcgcacgtt tgataggagt ggtgttcata atcctccttc tgagtatcgc tcttgtttcc
     2101 tgtgatgacg ctcttttgca gcacgtacca caaaatggaa cgcaaaacga agctcagaat
     2161 ggaacacaga ataatgatca gaatgaagag caggatgaag ccgaagagga agacgaagaa
     2221 tccgaagatg aaggcgatga atccggagat gagggtgatg agtccggaga tgaagacgat
     2281 gaattcgatg gactaccgcg caaaggggat tttacctaca ttggaactcc acccacaaat
     2341 catgccgcta tagtagaggc aagtaatgag ataaaagaag aaatggagag gatcaagagg
     2401 gagctcgatc atgccagaga aaatactgaa acgatcgatg acatatctgt cgagctcttc
     2461 aaaatgatgg ggaatgcttt caagaatttc gtagttgatt atgagaatga tggctatgcc
     2521 gggatggagg tgttttcagt tttattggaa ggagtagacg tcgagtctgt tgatgagaat
     2581 gccttactgc aggtggatgc cgattcagtg aaaaagtcta tgttcaccag aatacttttc
     2641 gacgaatcgg attcgaaatc catggagacc aaagtcggca tgagcgcaga gttcgaaatt
     2701 gtcggatcag agatggggat cgatggcgag tataagatca agctgcttat gaacgtcgag
     2761 ggtgatgatg aagagcaaga ggtgacactt gttggaacac tgacgaatat agccgaagac
     2821 actgaagaca ctgaaacgcc actgaccgaa gcggatctct tgctgggtga tgatgagtgg
     2881 tatgaatggg atcaggaggg cgcttatcct caccaaagaa ccctcactct gtttatggag
     2941 attgtgagtc aattcgatat gctatctgaa acgcttgcaa ctcagatttt ctaccccgag
     3001 atcacagggg ataatggcac agggaagttc atcgggttgg tgtctttcta tcaggaaggt
     3061 gatgctgctg aggaagagat tcctctgtat ctggctccta tgtatctatc ctttttgtgg
     3121 gagggggccg atcatgagaa atactatgtc gtatactcgt ctcacgaaga ttatcaaacg
     3181 atcgtagcga taaacggcgt ggcttacaac tttgcactac cataaccctg aaaagcaggt
     3241 gcgtgtggag gcttccccgt gagctttgat gcagggttca caggatttct tccggtatcg
     3301 aagtttctct ggaggcacca tttccctttc cggcaaggat agtcgggaag ggaatttctt
     3361 tgatcggtat tacattcaaa tataataaaa tatgtgaatt tccgtatttt tgagggttac
     3421 gaaatagacg gcacatatga cacatggtat aaagagaaaa aaggaatgac actgcaaaag
     3481 gagaggaaaa tgaagaaagt cgtacgtatg ctgggaggtg cttttgtgct cctcatcatg
     3541 gttttcatgg tcattactct tgtctcctgt gatgacgatc tattgccgaa tggggaacac
     3601 aatggatttc cggatctacc ggacaatgga tttccggatc caccggacgg atggcatgct
     3661 gaatggcccg atgattggaa gaatgaatgg ccgggagact ggccgggtga actgccggag
     3721 gatttttcat ttgagcttcc tgaagcgttg ccaagcacat gcgacggtac aagagcattg
     3781 atccagagcg tcgaagatgc aatcgttatt atcaagaatg acctgaacac ggcgaaggga
     3841 aacacggaaa taaatgatct ccccgcttta gatgagggat ctattcaata taaagcgaag
     3901 acagccttcg atggtctctt ggctaatcgg aatccaaagc acccggggat cactttgttc
     3961 cagttcggat tgtatggcga ctccacaaag gaaaatatat ccggcgtaac tatagatttt
     4021 gattccccgg aaggctgttt catggacatg atgaatgagc agggtgcttt catcaacatc
     4081 gagctggatt caaagttcac gatcaacgaa gcggaaggtg tccctgagga gctcccgggt
     4141 tcctacgaga tttcattgaa aggagccctc atggagaaag ctcccggaac cgatgtgatc
     4201 gtgataggaa gcacgttgga gaaaacaggc gaaggagctt ggaataaaaa tctgaccggt
     4261 accgatgtca agtttgatga tgaaggtgat tatgagtgga atcaggcggg cgctgagcct
     4321 caccataaaa tcctcacctt gctgatgagt tccttgggcg gagccaaacg gaaggaattc
     4381 gcgagaatgg cgttctattt cgatgtcacg tttgaaggcg tcgaaggaaa gttcattgcg
     4441 ggaatcactg catttggtgc tgaaagaaaa tcctattcat ccgtcatttg gaaagataat
     4501 gataacaatg aacatctcat cgtatggtcg aatcacaagg actatgaaga tttcatcgtg
     4561 atagacaaag tcgcttacaa gcttccgaaa acataggagt gtgcatggtt gcatccccgt
     4621 gagttctgga atacggtttc ggagcttttc agcagggatt ggttgccctc ccgattgtga
     4681 tgatccatga tggtcgggag ggactctttt gggtcgatac gttcatagcg atatgttttc
     4741 aaatcatagg atttcaacag gtggtgtgaa tggacggctc atggaagcca tgatgcatag
     4801 aaaaaaggtg tgaacttgcc actaaggaga ggcagatgaa gaaaatcgta cgtatgctgg
     4861 gaggagcatt agtgctcctt gtcatgatta ttgctcttgt ctcctgtgat gacaatgtag
     4921 cgccgaatga tgtgtctttt gacttcacta aaacgttgcc aaaaactcgc tccgaggcgg
     4981 aagccgcgat agacagcatc gaaaaggata tgaatgcaat caaggatgcg ttggacaatg
     5041 cgaagaaagg gacgcttgct ccttataagg aggaatttaa tgatctgata ggtgatctcc
     5101 ccggactgaa tccatacatg tccggaaccg agtcgtattg gtttggattg tatagcgatt
     5161 cttcagagat aaatcactat ggtataacgg atgcacctgt tacatccgca accgggtttt
     5221 ttgcagacaa gacgaaggga gctcattcgg atatcacgat cattgtggat acaaagttca
     5281 agatcaacgc agtgatggag gggaagccgg acattcgtgg atcatacgag atttcattga
     5341 atggaagcta cgtcgaggac gccggaataa tcgttccgat tgcaagtgga acgttgaaga
     5401 agaccgacgt gcccacttgg agcaagtcgc tgactaccgc cgatgttctg ttggatgaag
     5461 gtaaatataa gtgggataag aataagggtg agaatgagga taattatgaa cctcaccaac
     5521 gaatcctcac cgtcttcatg agtttgtcgc ttacctcaga gaaagcagcg atgaaagtgt
     5581 tttatacctc ggcaaaagat gaaggtgtca aaggggaatt cgtgtcagga tacacagtcc
     5641 atggcgaagg ggggtcttgg acatccgcta cgtggaagaa aggagacaag caatatacct
     5701 tcttgtattc cgatactgag gactatgatg atgtcattat cataaacgag gtggcatata
     5761 cagttgtaac atagtcttgg aaagcagttc gaagttttga attttgccct cccgattgtg
     5821 atggttccat ggcagtcggg agggattggt tggtggaaaa atgcaggacg taaagtcatt
     5881 ttcagccaca aatttacgct atatggttta gttttacgaa ttatgtccaa atgtgctgta
     5941 tttaccccaa gccggggtgg gttatgcagg ggaatcaaat ttagcacagg ttttaagaag
     6001 acaagagtca ataatgatgc ctgaaaagat caacttgggg agaggaaatc ttcggttgat
     6061 catgatttga tatcggaatg ctttcgagat cactttgtca atcagaggat taaagccata
     6121 tttagatttt cgcccggtgt attattcaac ccctgatgtc aaacaaatgc cacacatgga
     6181 aaagctcgat atcaggcttt gctgttttga aaatgtgaaa acagattttt attttcggct
     6241 tctcctcaag caagggcata aaaggaggta gcgggttttc tcttattcgg attctctttc
     6301 gacaggtctc catcataaca ccttgcaagc ttctctttca ttgatgtaca gtgttctggt
     6361 acggaaaagg gctgaacacg ggatgaggga gaggggattg tgagaaaact gattatcctg
     6421 tttgtcttcc tagtggtggg tttccagccg gtttccgcag gaactccgac gttccatgcg
     6481 ggggtttccg gaggtgtcgg cttcttttcg tggtacacct cgctggaagc gggaatcaag
     6541 gtggatctcc gtgagaactt cgccctcggt ctgacccagc gggtcgggta tggattcacc
     6601 tatcgggaga tcgtgggagg gacggaactt cgtgcttata tgtacgacga cctcttcatc
     6661 cacatcggag tctcctatct gttgaaacct tcttcttgtg cggaacccga tttcaagaca
     6721 atggttctgc ctcatctcgg cttcggtttg tacattccca tcgattctgg tcgcaggttt
     6781 ttcgtggttc cccgggtcga gatgaaccag tccttctatc tctccgactc gatccgaccg
     6841 gtctacagcg acctgccgtt tcttgtggca gggcaggcgt cgatcggatt tgaatatcga
     6901 atgagatgat agcatggttg cgtcgtggtt tcacaaaggc cttcagatcc aaaatcagcc
     6961 gtttccagat ggaagcgttt gactctgcat cgatataagg tgatgttgcg gagacgatct
     7021 acccaatcag cgctgttatc accttgatga tgtattctgc aaccactgtg ggactttctt
     7081 caattccgta tgtttggttc aaaaaatatt tcttcccttc tttatactga tattcgattg
     7141 aacgattttt gagaaatgtt tcaaggtcaa attcctcatt tgctcgtcta atgaatactc
     7201 caaagtgcca gcctgtgaga ttaagaattg aatcaatagc gatgcctacg ttaccaaaat
     7261 caatatctgt gactgctgta tcttctaaat atggtaaaac tctccaaggc cattgtctaa
     7321 ttttctttga actaattgca tcttctacta gtgagttaac ggatttgaca gttttccgca
     7381 tttcatattg gagcgatttt attttttcat tcattaaatt gaccgcatcc tgattttctt
     7441 tcacgaattt gatgaattca tagtccattg aatcccttcc ttgtagatta tcgatgtttt
     7501 tgaccaagtc gaagaataat gaaagataat tctgatttgc tcccactaca tattcaccca
     7561 ttcgccttcg aagctctttg aacagtttct cataggtgat gaacgtgtat ttcttattaa
     7621 taccgggatc caaaggtttc agactgagta caaaggcgat tactgttctc ccatttttgg
     7681 atgcaaactt gaaatactca ttcaaatcat tgtaaagaga ggcattaatc ttgttctcga
     7741 ttatgattcg atactcatct ccaattatat caatatcgat gaatttacct ttgtctgtag
     7801 gggtctcagt ttccacccta taattcaaat ccaagtcttt atgtccacaa aaggacaaca
     7861 gggtgtcaat gaatagattc cctaatccat gctctctttg gttatcgaga aagaatgcca
     7921 aaacattgga tatgactctt tcatagtgcg gaaaaccgca tatggagaat agattctcat
     7981 cttctcgtgg ctgatttggc aattccgaga atttttgaat cagttcttga aagtcggtga
     8041 tgcctgcatc catctttact cctttaacag ccccgtatcg tcatcgaatg atatacactt
     8101 tcttctatta ttatatatat ttttctacat ggagcaaatt attttcttac gggtataatt
     8161 cttttcttcg gtttctacct aagcagatta tctaggatat gtggtttcgt tcgccatcgg
     8221 ggattggctt tgaccttgag gtcgaggcgg agcttgcgct tggggaagat ggaacggatc
     8281 tccggttcga ccgccttgcg gatcttcgtc atattctctc cgcccttgcc gatcacgatg
     8341 cccttctggg attcccgctc gacgacgatg aacgcccgga tccagatcgt gttggtctct
     8401 tccttgtatt cgatgtccgc cacgtccacg tagatcgcat gaggaagctc ctcggtgacg
     8461 agggcgatcg ctttttcccg gatcagctcg gcgatccgga actccagctc ctggtcggtg
     8521 tacgcatcct cgtcgtagag aagcggacct tcgggggcga ccttgaaaag ctcgatcagg
     8581 acctcgtcga ttccctcgtc gtttctggcg cttccgatga ggatcgggga agaaggcaaa
     8641 tgctccttca gaaacgcttt cgtctcctcg atctcctcct cggtaaggat gtccgccttg
     8701 ttgatcaggc agatgaccgg ggttttcagt ttggcgagcg tccgggcgag cgaaagttcc
     8761 tcctccttgg cgctccgctt cccgtcaatg agatagagga ccaggtcgct ttcgctcagg
     8821 ctcttgaccg cggtctcctg aaggcggagg tttagaaccc gggtaccaag gtgatagccg
     8881 ggggtgtcga tgaagatcag ctggccccgc tgatcggtgt agatcccctt gatcgcattg
     8941 cgggtggtct gaggagtttt tgccgtgatc gagaccttca tctcgcagat cgtgttcacc
     9001 agcgttgatt ttcccgccga ggggcggcca acgaccgcta ccgttgcaga tttcatatct
     9061 tcagtatatc ttccttgccg gtgtgtggga atctcgtttt gatgcttttc atctccttgc
     9121 aatttgccta tacttgatat atccattcta tcgttcgaaa gtgaggccgt catggcggaa
     9181 gaaaaaaaga aaacgcaaag tgaagcacaa ctgcaggaaa agctgctgaa gacccgcagc
     9241 gttctgttaa gcggtgaaat caataaggaa agcgccgatg aagtgatcag gcagctcctc
     9301 gtcctggaag gggagagcaa tgagccgatc aggctctaca tcaacagccc cggaggggat
     9361 gtggatgccg gatatgcgat cttcgacatg gttcgcttca tcaacgcccc ggtgacgatg
     9421 atcgggatgg gccttgtggc gtccgccgcg gtgctcgtcc ttctggcggt tccttcagag
     9481 cggcggatcg ctctgccgaa ctcaacctac ctgatccacc aaccgatgag cggcatgagg
     9541 ggggtcgcca ccgacatcga gatccacgcc caataccttg aacgcctccg ctcgaagctc
     9601 gattccctga ttgcaagcga aaccggcaag agcgttgaag aggttgccaa cgataccgac
     9661 cgtgatcatt ggctgacgag cgaggaagcg ctttcgtacg gacttgtaag ccgcattgtc
     9721 gccgaacggt ccgcactgta atccgatatg cccaagacga agtatttaag cgacaaagaa
     9781 aaacgaattg gccaaaagca catgtatcgc caggaagtgt tcaatggcat ctccttcagc
     9841 ctgatgggcg agaccgtcgt gtatctgctt gcactctatt tcggtgccgg caatttcgcc
     9901 ctgggctata tctcctcggt gatatttctt gccggaataa tccttcccgt tgtcccccgg
     9961 atgctcaagg gaaagcatca caccaggacg caatccttcg tgtggcactt ccgcgggctc
    10021 gtgggactgg ggtacctcgg cctgttcatc gtcagtggag attgggcggt atttctgttg
    10081 ttgacggtgt ataccctcta taatctggta cggatgatcg ggatcgctct gaacgattcg
    10141 acgatgaaat cgatcagcag cccctccaat cggggcaagg tcgtggcgaa tgcgaacgtc
    10201 gcgtatcaaa gctcgtcgat gacggtccgc ctgatcatga ccgccgtctt cgctgttcag
    10261 cgcttcagcg gtcttgtagg cttggttacg atgcaggtgc ttggagtcgt cgccaactgt
    10321 ttcgcaagcc gcgaaataaa aaagattccc tcccggatgg tcatcaccca caagccgggg
    10381 cgaaccgtct ggacgttgtt caccgaagcg atggcccagc cggtgctgag gcgccggctc
    10441 ttgctgaaat ggcttgccac cggggtaacg gtcattttcg ggctcacgac cccgtttttc
    10501 cgcattgaac tgggttcatc caacgggatc gtcgtcctct attccgtgtt gctcggtctt
    10561 tcggtgatgg tcgcttcatg ggtgtcgaag cgattttccg accgtctcgg ctccaagccg
    10621 ctggttgtta tttcgacgct cttcaccttg gggtttttcg ccctttgggc gattcttccc
    10681 cgaaccctcc acttcgcctg gttctttggc ctcgggttcc tcacgaacac ctttgtggcg
    10741 ctgatcaacc tcttgacgtt ccgccttttg acccaggtga tgccggatga tgagcttgtc
    10801 tccttcaact cgatggtcaa cttcatcaac ggaatcgtcg ctttcggcgt cggaatgctc
    10861 agcgggtttt tggcgaactt cacccaagga agcttgctgt ttcatggcac ggcgctcgga
    10921 aacgggtata ccctcgtctt cattttcggt ttcgccctga tcctggtcga agctctggtc
    10981 gctctcagaa tccaggagat tggggcttac tcatcgcaag ccgccgccca ggtggtgttt
    11041 tcgatgcacg gaatccgggc ggtttcgatg atcgagcggc tggagcggac aagcgacccc
    11101 gccaagcggc ggatgctgat gcacaacctc ggggggaacg tgaactacct tgcgacaagc
    11161 gagctccgct cgattctcgc cagccccttc catgtcgaca agcttgaagc ggtcagggcg
    11221 atcggcgacc ggcctcgaaa atcactgctg gatgatctga tcagggtcgc ccaggatgac
    11281 gactcctatg ttcagcttga tgcgatcgcc gccctcggct cctaccggaa ggaagagaag
    11341 gcgaagagcg ccctcgtcga cctgctgctc catggccgat gggcttcggt ccgctcgatg
    11401 gcgtcaaagt cgcttgctcg gatcagcgat tcggatgagt acctcaacgt ggtcaatgag
    11461 ctcagccgtt cagccaagca tatcgacgaa gtgatcgact atctggtcgc caagcgcttc
    11521 atggacaaag aaggaaggtt ctttcaggag ttcttcatct tcgttgaaca gggacgcagc
    11581 ggcacgttcc gtcaaaccgg ctattcggtc gtcgcctccc tgcttcgctt cggtccgccg
    11641 agtctggcct ccctgtatga agagcgcaac ctgagctcga cgaaagcgta tcttgccggc
    11701 ttcctctccg aagcccgcga tctgacgatg atcgatcaga actacaacct catcattcag
    11761 atctttgcaa aagagcagtg gtccaagctg atcgatctct gcctggagtc cctgcgttca
    11821 agcgatgtgc aggcggattc gagtctggac aacctgaaga aaggattgct gaaggttgaa
    11881 acgatgagct tggaatcttt tgacgtggtc gacatgatcg cgctcctcta tttcacctat
    11941 ttcatctgca agagccagaa cagctgaagc gaagcggcgg cttttccccc ttacggggca
    12001 tgatcgtcct tcttctcatc ctcatcacct catgtttcgg atgctcgtgc gatattcccg
    12061 ccgatgagca tgtcttccgg gttctctcct acaacgtgga aaacctcttc gatgcgaccc
    12121 tcgacggaac ggagcatgcc gagtaccaag accccgggaa gtggagcgaa cgctcctatc
    12181 ggctcaggct caggaacctc ggcaagatcc tgcttgaccc ggctctcaac catcccgacg
    12241 tgatcatcct tcaggagatt gagaatgaac gggtcgtgga ggatctgctt tcctttcatc
    12301 tggcgaagaa ggggtaccgc tcgtatgcgg tcgcccggac ggagggttcg ccgatcagcg
    12361 tcggggcgat cagccgccat ccgatcgtcc gctcctcggt ccatgcggtt cccgggggac
    12421 ggaacatatt ggaagtcgtc atctcgactc ccgtcgaaga ggtcgtcatc ttcgccctgc
    12481 acgccaaaag ccagataggg gggttcagtg aaagcgagct ccttcgaatc gagaatgcga
    12541 aaagcgtctc cttggcgagc agggaccatg tcgataaagc ggtcctcgtc tgcggcgatt
    12601 tcaaccagaa tccgagggtc gtccatgaac acgagggagt gcagaccgcc ctcgttgacg
    12661 tgatccaccc gaatgcgaag ctctatggcc gggcggggtc gctgctgatc agcgggaatg
    12721 cggatttcct gggtccggga atcttctaca atccctacct tgccgaagac tgccgccttg
    12781 cagggtcgta ctgctatggg ggagtctggc accggtacga tcagatcctt ctttcccctt
    12841 cgctgttcga ccgcaggaac tgggagtatt ccaccttcga agtgatccgt ttcccctacc
    12901 tgctcaagag cgatggaact ccgaagcgct gggatctcaa aacgctcagc ggctacagcg
    12961 accatctgcc cgtgatggtc accctcactc ggagtccctg ggaaatttga gctcgtaaaa
    13021 gatctgctcc agctccaagg cgatgttgat gttgtggacg accggcatcc ggccatcttt
    13081 ccgggcttgg gctttgatcg tgatcggggt gaaattgaga atgcccatca ctccgctgcc
    13141 aaggagccgt tcgtagctgt cattggccgc ctgctcgggt acggcgagaa tccccacctt
    13201 tacatcgagg gctttgacga cctcgtcgat ccggctgagc ggatagatgg gaatcgggtg
    13261 ggaggagtcg ctgtagacca acggatcgct gtcgaaaccg gcgaccaccc ggattccgtc
    13321 ctgctcaaag ccgctgtagt gcatcagtgc ttttccgatt cgtccgcacc cgagaatgat
    13381 gcagttctgg ctgtctcctt tgcccaaaag ctccgccagc gaggcaagca gttcggagag
    13441 cgcgtagccg ccccgctttt ggccgtagat ccccagttgg gaaaaatctt tccgaaccgt
    13501 cgcagcggaa accccggcgg catcggcgag gttatgagcg aacacgcgtt caagaccgat
    13561 cgtcttcaga cggttgaggg tccggaaata tcgagttaca cgcaccaatt ggtcgttgtt
    13621 cattggattt tctccttata atatgaatga aagaatatta attttgaatt tagcgtaaac
    13681 atatcgtgtt atattcaaaa atacaatgcg tttgcttgga cagttttcag aatttatatg
    13741 tacatctttg cgtttaaggg gtaatgtatc accgaaacca gatggatact ggaggatcta
    13801 tgactgaaac aaacaccgta accttctccc ccgagcttgt gaatttcatc aaaacgtgga
    13861 aggacaagcc cggcaacctg atcatggtcc tgcaccaggt gcaggaagag cacggctaca
    13921 tctcccgcag cgcggcggat caggtggcgg cgatgctcga tgttccgctt gccacgattt
    13981 ggggggtcgt ctcgttctat cacttcttca agctgaccaa gcccggaaag cacaatatcc
    14041 aagtttgcat ggggactgcg tgctatctca aaggcggcca gccgttgatc gacgagctgt
    14101 cgaagcagct gaagatcggc gtcggtgaag tgacggagga tggaaacttt tcccttcaag
    14161 cagttcgctg cgttggatgt tgcggcttgg ctcccgtctt gactgtcgga gccgaggtgt
    14221 tcggaaaagt caccaaagac gagatcgcat caatcatcaa gcagttctcc tgacggaagg
    14281 cgtatgcata tcgcgatcac cgactacgtg cttgaagtgg tgcagaacgc gttcgaggcg
    14341 aacagcacaa acacgcgtct gatgctccaa gagaccgctg aagcgatcag cgtaacggtg
    14401 gcggacaacg gcaaggggat gagcgaagag gtgatgaagc gggcgcttga tccgttcagc
    14461 accgaagagg ggaagcaccc gcttcgaaag gttggactgg ggttgagttt tttaagtcag
    14521 gcgaccgagc ttgccggcgg gaaactgacc ctccaatcgg aagtcggggt gggaacgacc
    14581 gtccacttca ccttcgaccc cgcttcgatc gataccccgc cgctcggcga tgtggtgtcg
    14641 acgttcatga ccctgctcgc ccacccgaaa gcggaatcgc tggtgatcga acgaaaaacg
    14701 gaaaccgacg gatacgcgct ggaccgtcag gaattgatag acgtgctggg cgaccttgaa
    14761 atgagcggaa gcctgcactt gttgaaagag tacattgcga gccaagaggc cgcgttgagt
    14821 tgaggatact atggcaaaaa tgacacttga agagctgaga aggctccgtg aggaaaaaca
    14881 ggaagctctg gagaagcgct ccatcgaggg caaggacttc agaatcatca tcggcatggg
    14941 aacgtgcggg atcgccgccg gagcgaagga aacgctggca gcattccttt ccgttctgga
    15001 tgaggagaag atcaagcacg tggcgatcac ccagaccggg tgcatgggcc tgtgccacgt
    15061 cgagccgacg gtggaggtca ttcttcccga tatgccgccg gtgatctacg ggaacgtgga
    15121 tgcggatgtg gcgcgcagga tcgtggccga acatattctc gggaaacgcc tggtgacgga
    15181 ccatatgttc gatcgcccgg caatcgatat catcacacag ggtgggggca gataatggca
    15241 tttaaacact acatcctggt ttgcggcggt acggcctgcg agtcgagcaa agccgacgca
    15301 atctaccaga atctgatcaa agagtgcgaa gccaacggga ttgtcgatga ggtgcaaatc
    15361 gtcaagacgg gctgtttcgg cttctgtgag cagggaccga tcgtcaaaat cctgccggaa
    15421 gattccttct atgtgaaggt cgagccctcc gacgccaagg aactgatcgc cgagcacatc
    15481 atcaaaggcc ggcaggtcga gcggctgctg tatgacaagg acttgtcgaa gaagcacgcc
    15541 aaggtggagg acatccagtt ctaccagaag cagttccggg tcgtgctccg caactgcggc
    15601 acaattgatc ccgagaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc
    15721 ttggagatcg cgatggccca agcgcgtgag tacggtcttt taggtcaaaa tattcttggc
    15781 agcggctacg agttcgacat cgagatccgc cttggcgccg gagcgttcgt ctgcggcgag
    15841 gagaccgccc tgcttcgatc gatcgaaggc aagcggggaa tgcccacgcc ccgacccccc
    15901 ttcccggcgg tcaagggact ttggaatcga ccgacggtga tcaacaacgt tgaaacctgg
    15961 gcgaacatcc cggtgatcat ctccaagggc ggcaactggt tcaaccggat cggcaccgcc
    16021 gactcgaaag gaacgaaagt cttcgccctg accggaaaga tccggaactc cggcctggtc
    16081 gaagtgccga tgggaaccac cctccgcgaa atcatctacg acatcggcgg cggaattccc
    16141 aacaacaaga agttcaaagc cgtccagacc ggcggaccca gcgggggagt catcaccgaa
    16201 gactacctcg acaccccgat cgacttcggt tccctggtca aggccggctc gatgatgggc
    16261 agcggcggaa tgatcgtcat ggacgaggac gactgcatcg tcgacgtctc gaagttctac
    16321 ctcgatttca ccgtcgagga gtcgtgcggc aagtgtgctc cctgtcggat cggcgggcgt
    16381 tcgctgctca acattctgga taagatcacc ctcggcaacg ggacgatgga ggatttgaat
    16441 accctggaga agatcgggga agcgatgcgc aaaggctcgc tctgcgccct tggccagacc
    16501 gctccgaacc cggtcgcctc cacgctggct cacttcaggg atgaatacat tgcccatatc
    16561 gtggacaaga ggtgtccgag cggaacgtgc aagaatctgg tgacgtactc gatcaatccc
    16621 gagaaatgca ccggctgcac cctctgtgcc cgcaagtgtc cggtcaatgc aattaccggt
    16681 gaacggcgag agccgcacgt gatcgatcct tcggtctgca tcaagtgcgg agtctgtcat
    16741 gatgtctgtc gcttcggcgc cgtattggtc tcttaggagg acgcaaggtg agtgattttc
    16801 aagtaaaaat aaatgggatc tccgttgatg taacaagagg aatgacggta ttgcaggcgg
    16861 cgacgcaggc gggagtgaag attcccacgc tctgttacca cgatgacttg gtgccgttcg
    16921 gatcgtgcgg cctctgcatc gtcaagcagg agggttcgtc gaagatcatc cgggcgtgtt
    16981 ccgctccggt cgttgaagga ctgagcgtga ttacccacga taccgagctc tttgaggtca
    17041 ggaagacgat cctcgagctg attctttcaa cccacccctc tacctgtctg tcatgcatcc
    17101 gcaacgggga gtgcgagctt caggatctgg ccgccgaatt cggcatccgc gagcagccct
    17161 tcgaagtacg gctgagcgac cggccgaaag acacttccac cgcctcgatc gtcttcgagc
    17221 cggagaagtg catcaagtgc ggacgctgcg tccaggtgtg ccaggatctg caaggagtgt
    17281 acgctctcga gttcatcggg cgcggcgaca tgacggtgat ggccccggcg gcgatgatga
    17341 agctggagga cagcccctgc gtccgctgcg gccagtgcgt ggtccactgc cccgtcggcg
    17401 ccatttacga gaaggatcag atcgccgact acatcaaggc gatgaacgat cccgagaagc
    17461 agatcgttgt gcagatcgcc ccggcgatcc gggtcggctt gagcgaatcc ttcggccttc
    17521 ccgtcggaac cgtcacgaca aagaaaattt atgccgccat ccggcgcctc ggcgcggtgg
    17581 cggtgcatga tacgaacttc ggagcggacc tgacgatcat ggaggagggg agcgaactgc
    17641 ttggccggat gagcgacggc ggtctgcttc cccagctgac ctcctgctgt ccggcgtgga
    17701 tcgactatgt gcagaaatac taccccgacc tgcttgagca cgtctcttcg gcgaagagcc
    17761 cgatgcagat ggtgggagcg atccagaaga cctactgggc ggagaagatg ggaatcgatc
    17821 cggcgaagct gttcaccgtg gcgatcatgc cgtgcaccgc gaagaagcat gaaacgattc
    17881 gcgacaaggc gatgttctcc agcggacatc aggatgtcga ccttgtgctg acgacccggg
    17941 agttcgcccg tctgatcaag cgggtgggaa tcgatttcct ctcgcttcag gaagaggaag
    18001 cggacaaccc gatcgccgaa tactccggtg cggggacgat cttcggagcg accggagggg
    18061 tgatggaagc ggcgattcgg accgcctaca acgtcgttac cggaaaagag ctcgagaaca
    18121 tcgacgtcga agtcgtgcga ggccttgagg agatcaagaa agggacggtg gattttgacg
    18181 gcacgccggt gcgcgtggcg gtcatccacg gactgtcgca cgccaaggaa gtgatggatg
    18241 agattcgagc ggccaaagcg aagggtgaac gggctccata tgagttcgtt gaagtgatgg
    18301 cctgtcgggg cggatgcatc gccggaggcg gacaacccta cggcgcgacg gacatcctcc
    18361 gcaaggagcg ggcggaggga ctctatcagg atgaccggca gtccgccatc cgctgctccc
    18421 accagaacga ggcgattctg aagctctacg aggagtactt cgagaaaccg ctgagcgaca
    18481 aggctcacca cctgttgcat accacctatc aggagattcc tgtttacaag gcctgatagt
    18541 tggccagtat ccatttgtgc ccagccttcc ggctgggcac attcgattct tcgatgttct
    18601 gaggatcagt cgcagatctg atagagcaga agcgattgag cggtctcc
//
LOCUS       DAOSLI010000035        18622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3212_length_18622_cov_21.462487, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000035 DAOSLI010000000
VERSION     DAOSLI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 18622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18622
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3212_length_18622_cov_21.462487"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..1665)
                     /locus_tag="PLF67_06220"
     CDS             complement(421..1665)
                     /locus_tag="PLF67_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOE89644.1"
                     /translation="MQEERYFIGQILWDPTILNKTIVTPSDFQGVQERAIYKAMLALS
                     RENTVVDENSTAKKSGIPLSKVIDLKSSNIISSTWEHYQKVLIDASRVRAIKKTCERV
                     LESDLPADALIDIFIEETQDVRGRMIFRPSALPSCIEESIAALEARIKSKKIPGLATG
                     YPILDSFIGGLQKTRLYYLGARPSQGKSTLLMNLVANCNVPCIIFSAESSKSEFADRM
                     IIREKMISSSQYYNGTLTEAEAKKVMEASSELYDRNHIIIHDEPNIPLARLIQIARDA
                     KRYNKVEAIFIDYVQLLVFYDQSRPKNEQVSEISKQLKQLARELDVPVICAAQLRRDA
                     EGKKPQLSDFSDSTQLERDADVAIMIYHIDKKEGDDSNRETFLLIEKNRDGRLGYIRM
                     DYQPEYMLFREARDQQIPKKKD"
     gene            complement(1670..2425)
                     /locus_tag="PLF67_06225"
     CDS             complement(1670..2425)
                     /locus_tag="PLF67_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4373 domain-containing protein"
                     /protein_id="HOE89645.1"
                     /translation="MAKLAKRYINHDENASRDEKILKMREKYGARGYGLFWEIVEYLF
                     SNNGKAEINAKVVSLAIGEDVRTVRNFLSDCIEVYQLFETDGTYFWSKRLISHIDQII
                     AISAMKSKAVQKRYENKSVNLSLYNDIDRHGITNEPYTCTTDVLQLNNSTGTINKIKE
                     INKINDYCREKKPQQNNFLKPSIEEIEAYCMERKNAVDPIQFFDYYEANGWKVGRNSM
                     KDWKAAVRTWERNDSSGKKSKVVVQNDPYAELR"
     gene            complement(2413..2658)
                     /locus_tag="PLF67_06230"
     CDS             complement(2413..2658)
                     /locus_tag="PLF67_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89646.1"
                     /translation="MSGHPISTIQPTTTMIVEWYIHNKYGEINPDKQYPARDLLTAII
                     TILVNKHRHQQVQDMTFNQLLFAALTNDGRGVGQWQN"
     gene            complement(3120..>3487)
                     /locus_tag="PLF67_06235"
     CDS             complement(3120..>3487)
                     /locus_tag="PLF67_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89647.1"
                     /translation="NQLLDDKLAPFKDHVSNKSNDPWENYEYKVWEQVALENKLMIRA
                     SEQPGIYIIAVRSVKDFFDVLYHELKEYTKIHVKSKDPQAWYQFKPTDLELCEALRKD
                     KSLKKYAKITFQSTRNRPY"
     assembly_gap    3488..3587
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3588..4615)
                     /locus_tag="PLF67_06240"
     CDS             complement(<3588..4615)
                     /locus_tag="PLF67_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89648.1"
                     /translation="MKRSYVSQKINVIGENFRILVTMKVAGKKDSWDPLFLAMTKLQM
                     SIHAQFLTELINQLYESNSLLLSDTFEEEMEKLYQTLWDQCYASGDRFNYCSFSDFWN
                     RLLEQKNIPKEEIQDYYHAKMQIFIIWKTLITLYPFGQESIVDNYILKKDKDFIDGAF
                     YQGCSNTLLTRLIALMGSEMYIPVCHDWKNSTIPATIRPICHVSGIHEDKNELVISHG
                     PYGEKTLGDIYEDYSTMQPLDEFALQYMVFLHVVEHDLAQSIAQLSNAYACVISTLGG
                     ETDFLGQPWEQETQGHSDLHLEQLKKVFNQLLDDKLAPFLHLEQFKKLINQLLDDKLA
                     PFKDHVSN"
     gene            complement(4612..5922)
                     /locus_tag="PLF67_06245"
     CDS             complement(4612..5922)
                     /locus_tag="PLF67_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE89649.1"
                     /translation="MNYYLTKRKKAKRSYFYVNFTDPNDSRKILLSTSVELLRRKIGI
                     SSRLPVTREKEAYAIVERAIADGLINLDRDTKNFADYVLDFWDFDTSEYIRRRNQKAP
                     DSIGKDYAMNMLGCFKRHAQKYLPPKLNLHEVTASHIEKVIDSLFDEGVLSNSTVKRV
                     VQSMSVPLKEATRKKWIAHNPMDGVESIVNNHKERGIYSIEEIRKILDRLYMKGTIGV
                     TEIRKTRCKKNIWEKKPQTVKTNLKPYLAVMLAAYTGMRSGEIRALRSEQMRIVNDDF
                     CIIKVDRAVNDYAGEKNTKGKRARSVPIPRFLYDMLIKEASSNPHDGSSLVFWSKKSA
                     TNPISTNFLVKQLYKAQEAIGITEKQRLERNLDFHSLRHTFNTNLRGRISEKSLRAVV
                     GHESVEMSDLYTHATEEDVIAVGVAIVDIFKSIYEGKAGDGGST"
     gene            complement(6056..6127)
                     /locus_tag="PLF67_06250"
     tRNA            complement(6056..6127)
                     /locus_tag="PLF67_06250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6093..6095),aa:Val,seq:cac)
     gene            6204..7178
                     /locus_tag="PLF67_06255"
     CDS             6204..7178
                     /locus_tag="PLF67_06255"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOE89650.1"
                     /translation="MAILAVGSRVSKNEVGISFYFFNEQIGSLVHLGGISGIEQPTFQ
                     LFDRRERVLYSVSETINGSVSAYTIDEKLHAELLFSVPSGGDFPCHLSLCPFDELVGV
                     SNYGSGDFTLLTRQGKLFFKEHFEGVGFDPIRQEGSHIHSSLWSPDCSHLYVADLGLD
                     RIVRYSYDLHSKTVIELPEGTGPRHMAFGKDGLLYVVAELSSEVLVYRDGELQQRIST
                     LPPIFSGSNTASDLHFSKDGSYLYCSNRGHDSVAIFRVNHSLRRFEYCITDKEPRSFI
                     LSPDGLWMLVANASSNTITIHRRNQVNGSVGEKTQRLAVREPVCLTWM"
     gene            complement(7147..7797)
                     /locus_tag="PLF67_06260"
     CDS             complement(7147..7797)
                     /locus_tag="PLF67_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89651.1"
                     /translation="MTYIRKLIKVFLGIILYALGVVITIEARIGFSPWEVFHAGLANV
                     LNLQIGTISIGVGLILITITTIFGEKIGMATVLNIIFVGLTVNLILNAEIIPTSKRFS
                     FSIIQMTVGLFIMVFATYLYLSTGLGAGPRDAFMVLINRKTGIKVGIVRSLIEITVTI
                     LGALMGGLFGWGTIMAAVIIGIALQTVFRIFKFDPRTVEHEDIKTTLTSMLNRPAP"
     gene            complement(7794..9014)
                     /locus_tag="PLF67_06265"
     CDS             complement(7794..9014)
                     /locus_tag="PLF67_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE89652.1"
                     /translation="MYKSTIRACHLGSFIGALTVNLAPLLFVIFMTQFGLNFEQVGRL
                     VLLNFGIQIVTSLLFSSPVDRWGQKPFILFAHLMVFIGLILLALAPLLFTSIPYLGLA
                     FATIFYSVGAGLFELQLSAIIQSIPTDNKEGAMNLLHSFYAWGLIFVVVLTTTLLSVF
                     GKGSWPFIVLFWSIFPLYNFFNFLRVPFPPAVQAQERTKTVNLFKSKYFLLVLAGIFF
                     GGATELSISQWTSAFAESSLGLRKEIGDLLGVTLFALFLGGARTLYGALGKKFDLLKT
                     MTVGSMLCVGMYLLAAFSPSPLISLIACVLCGLGSALLWPGSVVNGANHFPKAGSSLF
                     AALAAGGAAGAAFGPWLVGLIASIKPDLVKIAPWLDFLTIEEAALRSGLFIGAIFPII
                     MIILLTLMRRENST"
     gene            complement(9042..12410)
                     /locus_tag="PLF67_06270"
     CDS             complement(9042..12410)
                     /locus_tag="PLF67_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE89653.1"
                     /translation="MKQRYSLSSITIARAPGFGANQFPVIEEFSDSLNIIYGPNGVGK
                     STLLRNLRALIYQRETETRSEAYGRLRIGEDVWELERSGKVVKQLRQRDGAIIDLPGV
                     NEEFRESYWFALHELVAPALQHHTVFFEMAKRQMQGGIDLDQAERELEAITQFAGKGL
                     KEARTVVAAKAKLNESKKRIGETKELGEEIQRVKTSLANRPELQARGKRLEEIRSYLE
                     LKHAYERQKAEVEKHDGRLASLRHTNLDVLSNLEKAAANARDSVEAGQKELLELTRKL
                     DELAVPEHLLGYKEVVDRLEDELDEAEDLHVKLQNGTENLIRKKGELESWNGQLGWLA
                     SEPPEEEALQKMVSSLGKLARTDEALRSAVQANAKLLKRYPKPTEDQKEYRSELIERK
                     HKLLELATGKKKLTKGEQAVYWILLSIIALLTGAGIYFGMNGFTREPIILGALSVMLL
                     SVLGRKVVWDLKREHTVDIKEFIRYFEAQPSTTYDQILQICFTELAELDEKMKVNDEV
                     QAEFEEAKKRYEEHIAAFEEIYEALGIPHDSALEGARFFNAGEHLRLWSNLLGSVRSQ
                     ETVVAELQASFDQRIEALEALCQSTFGNDPVREAKRFLDRLKQAQTTKETLERYQRSL
                     DESKEQLALAEAERRAWYERYEIESREVAEALFSRMEEYKSLADDLELQKRNLAKVDE
                     TIVEEARHIDRQTFDIEFEALRQTLEKLEDLHEHFITIQEQYRLASRASEHENALYEY
                     ESSLEALDAHRRDALKKRLVHSIIADLKEETEREHQPAVIQRSSAWLGRITHNRYSLN
                     AGKEAFRAFDHAEDRSLALEELSSGTRIQLLFALRMGYLESLEEGSNAALPIFFDEIM
                     ANSDDERSLAIAEAIGLIAEERQVFYATAQTDEVLKLKNQAETPPRLIDLEQLRSKEA
                     VERRPFTAPKIVPRKEIPFEEEYHQYAKTLMIAQAGLFDPVETLSSWYLCLDSKELQE
                     LLTRNLDKAGTARWSSPLLSRRFLLLKEARHLAQAGREKTLTAADLSDPELTLNRSTN
                     YYQSIETFVHRNKVTGSDLLQAIAEKKEIKNISADTLQTITEWLTKNGFLSEDGAYTK
                     EEIISLLSVKHEDLRIGSDEQLIVERYLSHILDP"
     gene            complement(12407..13618)
                     /locus_tag="PLF67_06275"
     CDS             complement(12407..13618)
                     /locus_tag="PLF67_06275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOE89654.1"
                     /translation="MKLLITADIHMGRIPSVGGLLNLSGTSAWKKIVDSAIEHEVDAL
                     ILVGDVVDDNELWFSVYGHILDGLNRLEEAGIRVFGVAGNHDSSVFARLSVDFPFLTI
                     LGQGGNWERVEFNGIELAGWSFPQETVAQNPFDQFTKTDSSKLTLGLLHTDLGGSQSK
                     YAPTKIENFQTSGIDLWMLGHIHKPGKIDSAEVYYCGSPFGLDRNETGVHGCWLVTSD
                     RLTRWDRIEQIELAPYRFESFTVELKNEESREEVRQTITRELQKRTRTADRTLFVRLK
                     LTGVVNRSLLDENLLEGDERYIELFAIQNGTIYLDRIYDQETTPPLDLEEIAQGSGPA
                     ALLARRILDPDSLNRLAETYSELEKASYRSGAYQQLAPETECEEEALKIAKEQALLVL
                     QSLMNQVESER"
     gene            13692..>14059
                     /locus_tag="PLF67_06280"
     CDS             13692..>14059
                     /locus_tag="PLF67_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOE89655.1"
                     /translation="MKHYRKELSFHLPHRRGLVNITSEVQKAIDESGIREGLVLVNAM
                     HITSSVFINDDESGLHHDWEVWLEGLAPEKPYSRYRHNGYEDNADAHLKRTIMGRETV
                     CAITDGKLDFGTWEQIFYYE"
     assembly_gap    14060..14159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14160..15245
                     /locus_tag="PLF67_06285"
     CDS             <14160..15245
                     /locus_tag="PLF67_06285"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOE89656.1"
                     /translation="GFRLSANIPYPAIVSSGSTFFFHTIEEVLAFIRRFPHPAVQIPK
                     MIREAAGLLASGKLVAFPTETVYGLGADATNVEAVKRIFTAKERPTFDPLIVHIAHLE
                     QLNGVVLDWDDRAQTLAEHFWPGPLTLVVKKDPAIDDLVTAGSRTVAVRMPANPIARS
                     LIALSGKPIAAPSANLFGQTSPTSAAHVHEQLAGRIDAIVDGGSCTVGIESTVLSLVS
                     EVPAILRPGKIGMKELLPFLPDLVLSPRIGIGAAPLESPGLLDTHYAPKTPLFLVEDL
                     SAYTRRKDLGILLFEESSLSFEGEVIFISRKADPLEAARTLYRAMRTLDQKGLTALIA
                     KRLPDEGIGLAINNRLEKAASKDLVKR"
     gene            15283..16494
                     /locus_tag="PLF67_06290"
     CDS             15283..16494
                     /locus_tag="PLF67_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE89657.1"
                     /translation="MNPTKELIYKKDTNLKLFVVTVLLTGLAYGLYRGVQDNYLAEIV
                     HITEFERGIVEFFRELPGLLLVFILAAMYRFSETNVFKVGTAVMLAGVLGLLFTGSSK
                     VVVILFMVVFSTGEHVVMPIKSSMSLYLAQKDKGGTSLGITSAIGKGGNILGYLVVSI
                     IFFVFARLGFSRQDTVAFKTVYALAAALLVGSTLVALAMKESGQRVKRSRLYVRRKFF
                     TFYMLEVFYGARKQIFLTFAPYVLILVYGATTTTISLLLAICALFGMLLSPAIGRIID
                     RLGYKVVMVADTLILMAVCLLYGFAHRLFPMHIAFYVVMVNFVLDSIISLASMASNVY
                     VMDLSDNREELTATLTTGISVNHFISVLIALLGGFIWKVVGIEVLFTISAILGVVNSI
                     FAATITKPKPR"
     gene            complement(16472..17377)
                     /locus_tag="PLF67_06295"
     CDS             complement(16472..17377)
                     /locus_tag="PLF67_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOE89658.1"
                     /translation="MTSTQFFRIVEMRTKIISMGTFFMASVYGYYLSGTVHWPIFFIM
                     LFATLAVDMGTTGFNTYFDFRRGTDSIEYTKESGKVLVHDGVAPNSALLISASLFLIA
                     AVLGLYLASMTSWYLIPVGGICMFVGFVYTGGPFPISRTPFGELFAGGFLGTVLFLIV
                     LFVQGFELSLANFLITIPLWIHIAMILSVNNTCDLVGDRANGRKTLAILLGPDKAPYL
                     ILVEGIIVYSGIVLLTLSGIYPLSAGIINFLALIVWIDSYRKMMKAGFDASTKEPNIA
                     LVSKAFLVFVFTFILGFALSAVLAW"
     gene            17596..18585
                     /locus_tag="PLF67_06300"
     CDS             17596..18585
                     /locus_tag="PLF67_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOE89659.1"
                     /translation="MKKSLVFAIILAFALLATPVFAAGQGEAAKSTGFDKSKYFVTVA
                     TGPTSGLYYPIGGAFSALYQNRLGYKSSAQATGASAENLTLLKDGRAEVAILMSDAVA
                     QAYEAYGPYEGQKPFSELRALLGLYPNYVQLVTTDKTGIKSFYDLKNKRVGVGAPNSG
                     VELNARLMYEAHGMTYADSKIDYLNYGEAINQLKNNQMDAVFVTSGIPNATIMELGTV
                     SKIVLIPIDGEGLANLMAKYPFFTPEIIPADTYDMYGDVATVTVRNILATTTAMPEEA
                     AYELTKGVFEHIADIQASHATAKKHISFENSHIGVGIPFHEGAERYYREMGWK"
BASE COUNT         4476 a         4716 c         4317 g         4913 t
ORIGIN      
        1 atactcctat cgattccata atccctatag gcacagaaag ctgcataggc tttggctgtc
       61 tctccctctt gtctctccca gataggagcc gtttggttct tatcctcgct cacattcatt
      121 ctctttgttt ttgacaagcc gcatgagctc ataggcaagg gtattggctt tgataaccaa
      181 ttctgcaact atcaataatt ccttaatagt aatcatttcc aagggtttct gaaccaggta
      241 ggaatgaatc cgttcaagta gtttagtgga actttgtaga agcatcactg agttgtctgt
      301 cattttgtca tttagaatag aactctccga acccataaaa cctcctctgt tttcattgac
      361 agtatataaa aaattgccat ttagatggtg tcaataggga cagataatac ctaggcgaca
      421 tcaatccttt ttcttcggta tctgttgatc tctcgcctct ctgaaaagca tgtattcagg
      481 ttggtaatcc atacgaatat acccaagcct gccatcccgg ttcttttcga tcagcaagaa
      541 cgtctcacga ttgctgtcat ccccttcctt cttgtcgata tggtagatca tgattgctac
      601 atcagcatcc cgttcaagct gtgtggaatc agagaaatcg cttaactgtg gtttcttgcc
      661 ctctgcatct ctacgcagtt gtgctgcaca gataacaggt acatccagct ctctagccag
      721 ctgtttcaat tgcttcgata tctctgatac ctgctcattc tttgggcgtg actgatcata
      781 gaacaccaaa agctgtacgt aatcgatgaa tattgcctca accttgttgt atcgtttagc
      841 atccctggca atctgaataa gtcttgccag cgggatgttc ggctcgtcat ggatgatgat
      901 gtggttcctg tcgtagagtt cactgcttgc ctccatcact ttcttggctt cagcttcagt
      961 aagggtccca ttgtagtatt ggctgctgct gatcatcttc tcgcggatga tcattctatc
     1021 cgcaaattct gatttgcttg actcagcaga gaagataatg cagggaacat tgcaattggc
     1081 cacaagattc atcaagagag tactcttacc ctgtgaagga cgtgcaccca agtaatacaa
     1141 gcgagttttt tgcagacccc cgataaaaga atccaggatt gggtatccag ttgcaagccc
     1201 aggaattttc ttactcttta ttcgggcttc aagggcagca atgctctcct cgatacagga
     1261 gggtagtgca ctgggcctga atatcatacg gcctctcaca tcctgggttt cctcaatgaa
     1321 gatatctatc aaagcatccg cgggcagatc actttcaagg actcgttcac aagtcttctt
     1381 gatggctctc acccgtgaag cgtcgattaa caccttctga taatgctccc aggttgaaga
     1441 aatgatatta ctgctcttga ggtcaatgac tttggatagt ggtattccac tcttctttgc
     1501 agtcgagttt tcgtcaacca cagtattttc ccgggagagt gccagcattg ccttatagat
     1561 tgctcgttct tgtacacctt gaaaatccga tggagtaacg atggttttat tgagtatggt
     1621 tgggtcccag agaatctggc caatgaaata gcgttcttcc tgcatccttc tacctcaact
     1681 cagcataggg atcattttgc acaactacct tggatttctt tccactgctg tcgttgcgtt
     1741 cccaagtacg aactgcagct ttccaatcct tcatggaatt cctgcctact ttccaaccat
     1801 tggcttcata gtagtcaaag aattggatgg gatcgacggc attcttgcgt tccatgcaat
     1861 atgcttcaat ttcttcgatg gatggtttta aaaaattgtt ttgttgtggt ttcttttccc
     1921 tacaataatc atttatttta tttatttctt ttattttatt tatagtacca gtgctgttgt
     1981 tcagctgtag tacatctgta gtacacgtgt agggttcatt tgtaattcca tgtctatcta
     2041 tatcgttata taaggataaa ttaacagatt tattctcata ccttttctga acagccttgc
     2101 ttttcatcgc ggatatagca atgatctgat ctatgtggga tatgagcctt ttcgaccaga
     2161 agtaggtccc atctgtctcg aacaattggt aaacctcgat gcaatcagac aggaagttcc
     2221 ttacagttct cacatcttcc ccgattgcca agctcactac tttggcattg atttcggctt
     2281 tcccattatt ggagaacaga tattcaacaa tctcccagaa gagaccgtag cctcgggcgc
     2341 cgtatttttc gcgcatcttc aggatcttct catccctcga tgcattctcg tcatggttga
     2401 tgtaacgttt ggctagtttt gccattggcc aacacccctc ccatcatttg ttaatgcagc
     2461 aaacagtagt tgattgaacg tcatgtcctg cacctgttgg tgacggtgct tatttaccag
     2521 tattgttata attgctgtca gtaaatctct agctgggtat tgtttatctg gattaatttc
     2581 tccatactta ttatgaatat accattcaac aatcattgta gttgtaggtt gtatggtgga
     2641 aatcggatga ccactcatct tcacgacctc ctactacgat ttccatacct gatttcatcc
     2701 aaggtaggta cttctcgttg ttccaaccag gcatggatct tatcgatgtc gaatcgaaca
     2761 tggccaccga tcttcaaata gggaatcgtt ttttgccata catactttcg gacagtcttc
     2821 tccgatatgc ccaataattc ggcaatttcc cgtacgttgt agtactgttt ttggggcctt
     2881 ggattattca tgcagctctc ctttccatca atgtgacaaa agaaaaaaga gatgcatgct
     2941 gatttggaaa gacatcaaat cccaagtaaa tacgtaccat aaagacaact ccttttcgcg
     3001 atgcattgct cgcgattcct gtgtttgtct ttattttaac ttttttagcc aactagagat
     3061 agattgcaaa aaccatgatt ttgtataggt tttttatatg tcgtatgttt gatagttcat
     3121 caataaggtc tgtttctagt tgattggaag gtaattttgg cgtatttttt gaggctttta
     3181 tcttttctta aagcttcgca gagttctagg tcagtgggtt taaattgata ccatgcttga
     3241 gggtccttag atttaacgtg aatcttggtg tattctttaa gctcatgata gaggacatcg
     3301 aaaaaatctt taactgaacg tacggcaata atatatatac caggctgttc agacgctctg
     3361 atcatcaact tattttcaag tgccacctgt tcccaaacct tgtattcata attttcccaa
     3421 ggatcgttag atttgtttga aacatgatct ttaaaaggag caagtttatc atccagtaat
     3481 tggttgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg tttgaaacat
     3601 gatctttaaa aggagcaagt ttatcatcca gtaattggtt gataagcttc ttgaattgtt
     3661 cgagatgcaa gaaaggagca agtttatcat ccagtaattg gttgaaaacc ttctttaatt
     3721 gttcgagatg caagtcggag tgtccttgag tctcctgctc ccatggctgg cctaaaaagt
     3781 ctgtttcacc acccaaagta ctgatcacgc aagcataagc attcgaaagt tgtgcaatgg
     3841 attgtgcaag gtcatgttca accacatgca agaataccat atattggagt gcaaattcat
     3901 caagtggttg cattgtggaa tagtcttcat atatgtcgcc aagagtcttc tcaccataag
     3961 gaccatgact gattactaat tcatttttat cttcatgtat gccagataca tgacaaatag
     4021 gtctaatagt tgcaggaatc gttgagtttt tccaatcatg acatacaggt atgtacattt
     4081 cactacccat caatgcaatc aagcgggtga gaagtgtatt actacatcct tgataaaaag
     4141 caccatcaat aaaatccttg tcctttttaa ggatatagtt atctactatt gattcctgtc
     4201 cgaaagggta cagagtaata agcgttttcc agataatgaa aatctgcatt ttcgcatggt
     4261 agtagtcttg tatctcttct ttgggtatgt tcttttgttc gagtagccga ttccaaaaat
     4321 cagaaaagct acagtaattg aatctatctc cagatgcata gcattgatcc caaagagtct
     4381 gatagagctt ttccatctcc tcttcaaacg tatccgaaag taataaggaa ttactttcat
     4441 acaattggtt tattaattcg gtaagaaatt gggcatggat ggacatctga agcttggtca
     4501 ttgccaaaaa cagcggatcc caagaatctt ttttccctgc aactttcatg gtaacaagaa
     4561 ttcgaaaatt ctctccaatc acgttgattt tttgactaac atacgagcgt ttcaagtact
     4621 acctccatcc ccagcttttc cttcatatat tgatttgaat atatccacaa tcgccacacc
     4681 tactgcgata acatcctctt cagtcgcatg tgtatagagg tcagacattt cgacactttc
     4741 atgcccgacg actgcacgca atgacttttc tgaaatccgt ccacgcaaat ttgtgttgaa
     4801 agtgtgcctc aggctgtgga aatctaaatt gcgttccagt ctttgctttt cagttatacc
     4861 gatagcttct tgtgctttgt agagttgctt aacaaggaaa tttgttgaga ttggattggt
     4921 tgcacttttt tttgaccaga aaaccaaact acttccatcg tgtggattag agctggcttc
     4981 ctttatgagc atgtcgtata aaaacctagg gatgggaaca ctgcgagcac gcttgccttt
     5041 tgtgtttttt tccccagcat agtcattgac ggccctatca actttgatta tgcaaaaatc
     5101 atcgttgacg atcctcattt gttcagatcg aagtgcccgg atttcaccag atctcattcc
     5161 agtatatgca gccagcatta ccgctaggta tggctttaag ttggttttga cggtttgggg
     5221 tttcttttcc catatgtttt ttttacaacg tgtcttccgt atctcagtaa cgccaatggt
     5281 gcctttcatg tagagtcgat ctaaaatctt acgaatttcc tctatagagt aaatcccacg
     5341 ctctttatgg ttgtttacga tggattcaac tccatccatc ggattgtggg caatccattt
     5401 tttcctggtt gcctctttga gaggaactga catgctttgt acaactctct tcactgtaga
     5461 gttagaaagg acaccctcat caaagagtga gtcgattacc ttctcgatgt gcgatgccgt
     5521 aacttcatga agattcaatt ttggcggaag atatttctgt gcatgtctct tgaaacaccc
     5581 cagcatgttc atcgcataat ctttaccgat gctatcggga gctttttggt tgcgtcttcg
     5641 gatatattca gaggtatcga agtcccagaa atcaaggaca tagtcagcaa agttctttgt
     5701 atcacggtcg agattgatca acccatcagc tattgctcgt tcaactatag catatgcttc
     5761 tttttctctg gtgacaggaa gtctgctact gatgccaatc tttctccgca acagctctac
     5821 acttgtagaa agtaatattt tcctggaatc attcgggtca gtaaaattga catagaagta
     5881 actgcgtttg gcttttttcc gcttcgtgag atagtagttc atactctttt ctactccata
     5941 aaactcactg ttcatatagc ctctgcatac ccctttacat acccttttgg gttttcacgt
     6001 caatattcac cgacaaaaaa gcaactaact ctttacggta gagttaattg ctttatgggc
     6061 gcgattgggt ttgaaccaac gacttctacc gtgtgaaggt agcactctcc cgctgagtta
     6121 cgcgccccga ggtgatggta gtctaagaaa aatatggctg gttgtccagt agcgccgggg
     6181 aatatatgtg ctatgatgat tgtatggcga ttttggcggt tggaagtaga gtctccaaaa
     6241 acgaggtggg gatttcattc tatttcttca atgaacaaat cgggagtttg gttcatctgg
     6301 gaggaatatc aggcatcgag caaccgacct tccaactgtt tgatcgaaga gaacgcgtcc
     6361 tgtactcggt gagcgagacg atcaacgggt ccgtttccgc ctatacaatc gatgagaaac
     6421 tgcacgccga acttcttttc tccgttccca gtggcggtga tttcccctgc catttgagtc
     6481 tctgcccttt tgacgagttg gtcggagtat ccaactacgg cagcggagac ttcacccttc
     6541 taacgaggca aggaaaactg tttttcaaag aacactttga aggtgtcggc tttgacccga
     6601 ttcgacagga aggatctcat atccactcca gcctctggtc tcccgattgc tcccatctct
     6661 acgtcgccga cctcggcctt gatcgcattg tccgctattc atatgatctg cacagcaaaa
     6721 cagtcatcga gctccccgaa ggcaccgggc cgcggcacat ggcattcgga aaagacggac
     6781 tgctctatgt cgtcgccgag ctttccagtg aagtcctggt ctatcgtgac ggagagcttc
     6841 aacaacgaat cagcactctg cctccaattt tcagtggaag caatacagcg agcgaccttc
     6901 acttcagcaa agacggatca tacctctact gttcgaatcg ggggcatgac tcggtggcga
     6961 tttttcgagt gaaccacagc ttgcgccgtt tcgaatactg catcaccgat aaagaaccga
     7021 gaagcttcat cctttcaccg gatggcttat ggatgctggt cgccaatgcc tcaagcaaca
     7081 cgattacgat tcaccgccgc aaccaagtca acggttcggt cggagaaaaa acccagcggt
     7141 tggccgtcag ggagccggtc tgtttaacat ggatgtaagt gtcgttttga tgtcttcatg
     7201 ttcaacggtc cgagggtcga atttgaagat tcggaatact gtctgaagag cgattccgat
     7261 aatgacagcc gccatgatcg tcccccatcc gaacaggccg cccatcaggg cgcctaagat
     7321 tgttacggtg atttcaatca gggaacgaac aatgcctact ttgattccgg tctttcggtt
     7381 gatcagcacc atgaacgcgt cccgcggccc cgctccaagg ccggtagaga ggtacaggta
     7441 ggttgcaaac accatgatga agagcccaac cgtcatctga atgattgaaa aggaaaaacg
     7501 tttggaagtc ggaatgattt cggcgttgag aataaggttc acggtcaatc cgacaaagat
     7561 aatattgaga accgtcgcca ttcctatttt ttctccaaag atggtggtga tcgtgatcag
     7621 aatcaaccct actccgatgg agatcgttcc tatttgaagg ttcaatacgt tggcaaggcc
     7681 ggcatgaaac acctcccacg gagaaaaccc gattcgggct tctatcgtga ttacaacccc
     7741 taaagcgtag agaatgattc cgagaaagac cttgatgagt tttctgatgt acgtcatgta
     7801 ctattctcgc gcctcatcag ggtaagcaga ataatcatga tgatggggaa gatcgctcca
     7861 ataaacagtc cgcttctcaa ggctgcttcc tcgatggtga ggaaatccaa ccagggggcg
     7921 atctttacga gatccggttt gatgcttgca atcagtccga ccagccacgg accgaacgcc
     7981 gctccggccg ctccgccggc ggcaagcgca gcaaacaggg aagaaccggc tttgggaaaa
     8041 tggttcgctc cattgaccac cgagccgggc caaagaaggg cgctgccaag accacagagg
     8101 acgcaggcga tcagactgat gagcggagag ggactgaatg cggcaagcag gtacatcccg
     8161 acacagagca tggagcccac cgtcatcgtt ttcagaagat cgaatttttt tcccaacgct
     8221 ccgtagaggg tgcgggctcc tccaagaaag agggcgaaaa gggttactcc aagcagatct
     8281 ccgatctctt ttctcaagcc cagtgacgat tcggcaaatg cgctcgtcca ctgggagatt
     8341 gagagttccg tagccccacc gaagaaaatt cccgcaagga caagcagaaa gtactttgat
     8401 ttgaaaaggt tgaccgtttt cgtccgttcc tgagcttgaa ctgccggagg aaacgggacc
     8461 cgaagaaaat tgaaaaaatt gtagaggggg aagatcgacc agaacagcac gatgaagggc
     8521 catgagccct ttccgaagac ggatagaagc gtcgtggtga gcaccaccac gaagataagg
     8581 ccccaggcgt agaaggagtg cagcaggttc atcgctccct ccttgttgtc cgtcgggatc
     8641 gattggatga tcgcgctgag ctgcaactcg aaaagacccg ctcccaccga atagaagatc
     8701 gtcgcaaatg ccaagccgag gtaaggaatc gaggtgaaaa gcagcggggc aagagccaga
     8761 agaatcaatc cgatgaagac catcaggtgg gcaaaaagga tgaacggctt ttgtccccat
     8821 cgatcaaccg gggaactgaa gagcaggctt gtgacgatct ggatgccgaa gttcaggagc
     8881 accaagcgac cgacctgttc gaagttcagg ccgaactgcg tcatgaagat gacgaacaac
     8941 agcggtgcga gattgaccgt caacgcaccg atgaaactgc cgagatggca cgccctgatc
     9001 gtggatttgt acatagcttc gagtataggc gataaccggc gctaggggtc aaggatgtgg
     9061 gaaagatatc gctccacgat gagctgttca tcacttccga tcctcaaatc ctcatgcttg
     9121 acgctgagca gcgagatgat ctcctccttc gtatacgccc catcctcgct gaggaaacca
     9181 ttcttcgtca gccattcggt gatcgtctgc aaggtatcgg ctgagatgtt cttgatctcc
     9241 ttcttctcgg cgatcgcctg aagcaagtcg gagccggtaa ccttgttccg atgtacaaag
     9301 gtttcgatgc tctgatagta attggtcgat cgattcaggg tgagctccgg atcgctcagg
     9361 tcagcggctg tcagtgtttt ctcccgtccc gcctgagcca gatgccgggc ctccttcaag
     9421 aggagaaacc gtcttgaaag caacggtgag ctccatctcg cagttcccgc cttgtcaagg
     9481 ttacgcgtga ggagctcttg caactctttg ctgtcaagac aaagatacca cgaagaaagg
     9541 gtctcgacgg gatcgaagag gccggcttga gcgatcatca acgtcttggc gtattggtgg
     9601 tattcctctt caaaggggat ctcttttctg ggaacgatct ttggtgcggt gaacggccgc
     9661 ctttcgaccg cctccttcga gcgcagctgc tcaagatcga tcagacgagg aggggtttcc
     9721 gcttgatttt tgagcttaag cacctcatcg gtctgggctg tcgcatagaa gacttgccgt
     9781 tcttcggcga tcaagccgat cgcttcggcg atcgccagcg atcgttcatc atcgctgttc
     9841 gccatgattt catcaaagaa gatcggaaga gccgcattgc tcccctcctc cagactttcc
     9901 aagtacccca ttctcagagc gaaaagcaac tggatgcgcg ttccgctgga gagctcttca
     9961 agagcaaggg atcgatcttc ggcgtggtcg aacgcccgga acgcctcctt cccggcattc
    10021 agtgaatatc ggttgtgagt gatccgccca agccatgccg acgaacgctg aatgaccgca
    10081 ggctggtgtt cccgctcagt ctcttccttc agatcggcga tgatcgagtg gacaagccgt
    10141 ttcttaaggg cgtccctacg atgggcatcc aacgcttcca gcgagctttc gtactcatac
    10201 agggcgttct catgctcgga agccctgctc gccaaccgat actgctcctg aatcgtaatg
    10261 aaatgctcgt ggagatcttc aagcttctcc aatgtttgcc gaagcgcttc aaactcgata
    10321 tcgaacgttt ggcgatcaat gtgccgtgct tcctccacaa ttgtttcatc aaccttggca
    10381 aggttccttt tttgcagttc aaggtcgtca gcaagactct tgtactcctc catccggcta
    10441 aagagcgctt cggcgacctc ccggctctcg atctcataac gctcatacca tgctcgccgt
    10501 tcagcctcgg caagagcaag ctgctccttg gattcatcaa gcgatcgttg ataacgttca
    10561 agcgtctctt tcgtcgtttg ggcttgcttc agccgatcca gaaagcgctt cgcttcacga
    10621 accgggtcat tcccaaacgt ggattggcaa agcgcttcaa gggcctcgat ccgctgatcg
    10681 aatgaagctt gaagttcagc gaccacggtc tcttgggaac gaacggaacc gagcaggttt
    10741 gaccagagtc gaagatgctc ccccgcattg aagaaccggg cgccctccaa cgcggaatca
    10801 tgcggtattc ccagcgcttc gtagatctct tcaaaggcgg cgatgtgttc ctcatatcgc
    10861 ttcttcgctt cttcgaactc cgcctgtact tcatcattga ccttcatctt ttcatcgagt
    10921 tcggcaagct cggtgaaaca gatctgcaga atctggtcgt acgtagtcga tggttgggct
    10981 tcgaagtatc ggataaactc cttgatatca acggtatgtt cgcgcttaag atcccagacc
    11041 acctttcggc cgagaacgga aagcagcatg accgataacg caccaaggat gatcggttcc
    11101 cgggtgaacc cgttcatccc gaaatagatt ccggcaccgg tcaagagagc gatgatggaa
    11161 agcagaatcc aatataccgc ctgttcacct ttggtgagct ttttcttccc cgtagccagt
    11221 tccagaagtt tatgctttcg ctcgatcagc tcgctccggt attccttctg atcctcggtg
    11281 ggcttcggat accgcttcag cagcttggca ttggcttgga cagcgctccg caatgcttca
    11341 tcggtccggg cgagcttacc caaggaggag accatcttct gaagcgcttc ctcctccggc
    11401 ggttcggaag cgagccatcc gagttgtccg ttccagctct ccagctctcc cttcttccga
    11461 atgaggttct ccgtaccgtt ttggagcttg acatggaggt cttccgcctc atcaagttca
    11521 tcctcaagcc ggtccaccac ctccttgtaa ccaagaagat gctcaggaac cgccagctca
    11581 tcgagtttcc gagtgagttc caaaagctcc ttttgtccgg cttccacgga atcccgagca
    11641 ttggctgcag ctttttcaag gttggaaagc acatcaaggt tcgtgtgtct caacgaggcg
    11701 agtcgtccgt catgcttttc tacctccgct ttctgccgct cataggcatg tttcagttca
    11761 agataggaac ggatctcttc cagcctcttt cctcttgcct gaagctccgg ccggttggcc
    11821 aagcttgtct tcacccgttg aatctcttca ccgagctcct tggtttcccc gattcgtttc
    11881 ttggattcat tcagcttcgc tttggcggcg acgacagttc gcgcttcttt gagccctttt
    11941 ccggcgaact gggtgatcgc ttcgagctct ctttccgcct gatcaaggtc gattccccct
    12001 tgcatctgac gcttcgccat ttcaaagaag accgtatgat gttgcagcgc cggggcgaca
    12061 agctcgtgaa gtgcgaacca ataggattcg cgaaactctt cgttgacccc cggaagatcg
    12121 atgatcgctc catcccgctg acgcagctgc ttcacgactt ttccgcttcg ttccaactcc
    12181 cagacatctt ctccgattct cagtcgacca tacgcttcgc tccgggtttc ggtttcacgc
    12241 tgatagatga gggcgcgaag gtttcgcaag agcgtggatt taccgactcc atttggaccg
    12301 tagatgatgt tcagggaatc gctgaactcc tcgatgacgg ggaattgatt cgctccgaaa
    12361 ccgggagccc gagcgatggt gattgatgat agggaatatc gctgtttcat cgctcactct
    12421 ccacttggtt catcagcgac tgaagaacaa ggagggcctg ctcctttgcg atcttcaaag
    12481 cttcctcctc gcattcggtt tcaggtgcca gctgttgata tgctcccgag cgataagagg
    12541 ctttttccag ctcactgtag gtttccgcaa gccggttcaa ggaatcggga tcgagaatcc
    12601 gtcgggccag cagggcggcg ggaccgctgc cttgagcgat ctcttcaagg tccaacggcg
    12661 gcgtcgtttc ctgatcgtag attcgatcga ggtatattgt tccgttctgt atggcaaaca
    12721 gctcgatgta tcgttcatcg ccttcaagaa gattttcatc gagaagactc cggttgacga
    12781 ccccggtcag cttaagtcga acgaacaagg tgcggtcggc cgttcgggta cgtttctgaa
    12841 gctcccgggt aattgtttga cgaacctcct cccggctttc ctcattcttc aattcgacgg
    12901 tgaaggactc gaagcgatac ggggcaagct caatctgctc gattcgatcc catcgggtca
    12961 aacgatcact cgttaccagc cagcagccgt gaacacccgt ttcattccgg tccagtccga
    13021 agggcgagcc gcagtagtac acttcggctg aatcgatctt tcccggtttg tgaatatgac
    13081 caagcatcca cagatcgatt ccgctcgttt ggaagttctc gattttcgtc ggagcgtact
    13141 tgctttggct tccgccaagg tcggtatgca acagaccgag cgtgagcttt gaggaatctg
    13201 tttttgtaaa ctgatcaaat ggattttggg caacggtttc ttgggggaac gaccatcctg
    13261 ccagctcgat cccgttgaac tccacgcgct cccaattccc cccttgacca agaatcgtga
    13321 ggaaggggaa atccacgctc aatcgggcga ataccgagga gtcatgattc cccgcaaccc
    13381 cgaacaccct gattcccgcc tcttcaagac ggttcagccc atccagaata tggccataca
    13441 cggaaaacca gagttcattg tcgtccacga catctccgac aaggatcagg gcgtcaactt
    13501 catgttcgat cgcgctgtcg acgatctttt tccatgccga ggttccactg aggttcagca
    13561 gtcctcccac gcttggaatc cgtcccatat ggatatcggc agttatcaat agtttcatct
    13621 tcactccatg gatgagtata ccatagacct accttaccgc ttcaggtatc gtagagcaag
    13681 gagaaacgac tatgaaacac tatcgaaaag agttgagctt ccatctgccc caccgcagag
    13741 gcctggtgaa catcacctct gaagttcaaa aagcgatcga tgaaagcggc atccgggaag
    13801 gtcttgtgct ggtcaatgcg atgcacatca cttcaagcgt cttcatcaac gatgacgaat
    13861 ccggcctcca tcacgattgg gaggtatggc tggaaggact tgcgccggaa aagccctaca
    13921 gccggtatcg gcacaacggg tatgaggaca acgccgacgc ccacttgaag cggacgatca
    13981 tgggccgcga gacggtctgt gcgatcaccg acggaaagct ggatttcgga acgtgggagc
    14041 agatcttcta ctacgagttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    14161 ggttccgcct ttcggcgaac atcccctatc ctgcaatcgt ttcatccggc tccacgttct
    14221 ttttccacac gattgaggaa gtcctcgcct tcatcagaag attcccccat cccgccgttc
    14281 aaatccccaa gatgatcagg gaagcggcgg gcctcctcgc atccggcaag ctggtggcgt
    14341 ttcccactga aacggtgtat ggcttgggag cggatgcgac caatgtggaa gcggtaaaac
    14401 ggatcttcac agcaaaggaa cgccccacct tcgatcccct gatcgtccat atcgcccatc
    14461 ttgaacaact caacggtgtc gtactggatt gggatgaccg cgcccaaacc ctggccgaac
    14521 acttttggcc ggggcccctt accctcgtgg tgaagaaaga tccggcgatc gatgacctgg
    14581 taacggcagg cagccggacc gttgcggttc ggatgccggc caacccgatc gcccgcagtc
    14641 tcatcgctct aagcggaaag ccgatcgccg ctccttcggc gaaccttttc ggacagacga
    14701 gtccgacaag cgcggcccat gtgcacgaac agcttgccgg gcggattgat gcgattgtgg
    14761 acggcggctc ctgcacggtc ggaatcgaat ccaccgttct ctccctcgtc tccgaggttc
    14821 ctgcgatcct ccgtccgggg aaaatcggaa tgaaagaact cctgccgttt cttcccgacc
    14881 ttgttctttc tcctcgaatc ggaatcggtg cggcccccct tgaaagcccc ggtctccttg
    14941 atacccacta cgctccgaag acaccgttat tcctcgttga ggatctttcc gcatataccc
    15001 gtcggaaaga tcttggcatt ctattgtttg aggaaagctc cctttccttt gaaggtgaag
    15061 tgatcttcat cagcaggaaa gccgatccct tggaagcggc ccgaaccctc taccgggcga
    15121 tgagaacgct ggatcaaaaa ggtcttaccg cgctgatcgc caaacgcctg cccgatgaag
    15181 gaatcggcct ggcgatcaac aatcgcctcg aaaaagccgc ctcaaaggat cttgtcaaac
    15241 ggtgattgac agtcgaaggg cctttgtcca tagtcctttt acatgaaccc aacaaaagaa
    15301 ttgatctaca aaaaagatac aaacctcaaa ctctttgtcg tgaccgttct gctcaccggg
    15361 ctcgcctatg ggctgtatcg gggagttcag gataactacc tggccgaaat cgtccatatc
    15421 accgagtttg agcggggcat cgtggagttc tttcgtgaac ttcccggcct cctcctggtc
    15481 ttcatcctcg ctgcaatgta tcgcttcagt gaaacgaatg tcttcaaagt cggaacggcg
    15541 gtaatgcttg ccggtgtcct cggacttctg ttcacgggtt cttcaaaagt tgtagtcatc
    15601 ctgttcatgg tcgtcttttc caccggcgag cacgtcgtga tgccgatcaa gtcttcgatg
    15661 agcctctatt tggcccaaaa agacaaggga ggaacctccc ttggaatcac ctcggcgatc
    15721 ggcaaaggag gaaacatcct cggctatcta gtggtgagca tcatcttctt tgtctttgcc
    15781 cgccttggct tctcccgtca ggataccgtg gcgttcaaaa ccgtctatgc tctcgctgcc
    15841 gctcttttgg tcggatccac gctggttgcc cttgcgatga aggaaagcgg acagagggtg
    15901 aaacgaagcc gcctctatgt tcgacgaaag ttttttacgt tctatatgct tgaagtgttt
    15961 tacggtgccc gcaagcagat ctttctcacc tttgccccgt atgtcctcat tctggtgtac
    16021 ggcgcgacca caacgacgat cagtttgctc ttggcgatct gtgcactctt tggaatgctt
    16081 ctctccccgg ctatcggtcg gatcatcgat cgccttggct ataaagtcgt gatggttgcc
    16141 gacacgctca tcctgatggc ggtatgcctc ctctatgggt tcgcccaccg cctctttccg
    16201 atgcatatcg ctttttatgt cgtgatggtc aacttcgtcc tcgattcaat catcagtctg
    16261 gcgagcatgg cgagcaacgt ctatgtgatg gatctttccg acaatagaga agagcttaca
    16321 gcgacgctga ctaccggaat ctccgtcaat cacttcatca gcgtcctcat cgcccttctt
    16381 ggcgggttca tttggaaagt agtcggaatc gaggttctct ttacgatttc agcgattttg
    16441 ggagtcgtca actcgatctt cgccgctacg atcaccaagc caaaaccgcg ctgagcgcaa
    16501 aaccgaggat gaaggtaaat acgaaaacca gaaacgcttt gctgaccaag gcgatgtttg
    16561 gttctttggt cgaagcgtca aagcccgctt tcatcatctt ccggtaggag tcgatccata
    16621 cgatcaacgc aagaaaattg atgattcccg cactgagggg atagatcccg ctgagcgtca
    16681 acagaacgat cccactatat acgatgattc cttcgacaag aatcaaatag ggcgctttat
    16741 caggaccgag gagaatcgca agcgtctttc gtccattcgc cctgtcaccg accagatcgc
    16801 aggtgttgtt caccgagaga atcatagcga tgtgaatcca caggggaatc gttatcaaaa
    16861 agttcgccaa ggaaagctca aacccctgga caaaaagcac aatcaaaaag agaaccgtac
    16921 ctaaaaaccc gcctgcaaac aattcaccga aaggggttcg ggagatcgga aacggacctc
    16981 ccgtgtatac gaatccgaca aacatgcaga tgccgcccac cggaatgaga taccaactcg
    17041 tcatcgaggc aagatacaag cccagcacgg cggcgatgag aaagaggctt gccgaaataa
    17101 gcaaggcgga gttgggcgca accccatcat gcacaagaac cttcccgctc tccttggtgt
    17161 actcaatgct gtccgtacct cggcggaaat caaagtaggt attgaaaccg gtcgtcccca
    17221 tgtcaaccgc caaggtagcg aagagcatga tgaaaaagat cggccaatga acggttccgg
    17281 aaaggtaata tccatagacg cttgccatga aaaacgtgcc catgctgatg atcttcgttc
    17341 gcatttcaac gattctaaaa aactgagtac tcgtcatacg gcaatcataa ttaatgcgca
    17401 taagtatgcc tagagggatg acttcaaccc ttgacaatct cctgcccatt atcacattct
    17461 acccctatgt cttgtaatcc atcggataga tttggtataa atatacattt aaacgtatgt
    17521 gagctgtcag ccgcatgcaa cgacacatac aggcgaaacc attatgtttc cctgatataa
    17581 tggaggaact tccttatgaa aaaatcactc gtttttgcta tcatccttgc attcgcactg
    17641 cttgcaacgc ccgtattcgc tgcaggacag ggggaagcgg ccaaaagcac cggctttgat
    17701 aagagcaagt attttgtcac ggtcgctacc ggaccgacca gcggtctgta ttacccgatc
    17761 ggaggagcat tcagcgccct ctatcaaaac cggctcgggt ataaatcttc cgcccaggca
    17821 accggggcat cagcggaaaa cctcaccctg ctgaaagacg gccgggccga ggtcgccata
    17881 ctgatgagtg acgctgtcgc ccaggcgtat gaggcgtacg gtccctatga aggccaaaaa
    17941 ccgttcagtg aactgagagc ccttctcggc ctctatccga actacgttca gctggtcacc
    18001 accgacaaga ccgggatcaa atcgttctat gatttgaaaa acaagcgcgt cggtgtggga
    18061 gcccccaaca gcggcgtcga gctgaacgcc cgactgatgt acgaagcgca cggaatgacc
    18121 tatgccgaca gcaagatcga ttatctcaac tatggggagg cgatcaatca actgaagaac
    18181 aaccagatgg atgcggtgtt cgtcaccagc ggcataccga acgccacgat catggaactc
    18241 gggacggtct cgaagatcgt attgatcccc atcgacggtg aaggattggc aaacctgatg
    18301 gcaaagtacc cgttcttcac tcccgagatc attcccgccg atacgtatga catgtatggc
    18361 gatgtcgcca ccgtaaccgt tcggaacatc cttgcaacca ccacggcgat gcctgaagag
    18421 gcggcgtatg agttgacgaa aggcgttttt gaacacattg ccgatattca ggcctcccac
    18481 gccaccgcga agaagcacat ctcctttgaa aacagtcata tcggtgtcgg cattcctttc
    18541 cacgaaggag ccgaacgcta ctatcgtgag atgggctgga aataaatgaa gataagagtg
    18601 gcattgttgc tgctcgttcc gg
//
LOCUS       DAOSLI010000036        18458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3236_length_18458_cov_24.427593, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000036 DAOSLI010000000
VERSION     DAOSLI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 18458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18458
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3236_length_18458_cov_24.427593"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PLF67_06305"
     CDS             complement(<1..1663)
                     /locus_tag="PLF67_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOE89660.1"
                     /translation="MHKRFLPIVTVIIVLSVFLLISCSDEIQMPQKPGIRGRISLPAS
                     SELAPEEIWVKAVEGETTKYVGRVNPDGTFLIPNLDSEKKYNIHFTSIEPEFENKRSV
                     GDRAAGRKPYGGWLTDVSASIDASVGSVKMKPLGTIMGTAIRTGAADNYDVMVYIPGT
                     SLMAMTDAEGNFSITNVPQGTHRIRYTSSGYLSLMKEGVVLASDSDDESPTKVVAEAS
                     LIREAGIVRGTVTLKGAQSHEGIIIRLEGADPEDVATGTTTEDGAFLIPDVKPGTYSA
                     LISYPGHVPQRLEGITVRAAETTSIGQAVALSANGGDITGKITLNDGKPLSGAAVLAT
                     ITIDGKRYSFSTTTDAEGTYVLNNCPLGEGYEISIAKMGYVTVIETGVSVSAGVPTVI
                     PETILISSSCAITGSVVLEGSPGNSGVTVSATLVGDQGRVVTTTTLEDGTYYIGGLDV
                     GQYSIQIQMNGYVTDRSKLVHTTYGETTVIDPVVLKSVKATVKGSVTLIGTNVHTGIS
                     VMLKTPDNQLQYDATTAQDGAFVVTGVQPGIYSLYVSKAGYESKAV"
     gene            2182..2379
                     /locus_tag="PLF67_06310"
     CDS             2182..2379
                     /locus_tag="PLF67_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HOE89661.1"
                     /translation="MEKLKRIGSQLLLEEFIEALTKWAMAIIVAHNHPSGVLELSVED
                     REETRRLKKAGDILGIPIILG"
     gene            complement(2445..3659)
                     /locus_tag="PLF67_06315"
     CDS             complement(2445..3659)
                     /locus_tag="PLF67_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89662.1"
                     /translation="MGGCFDASSANEGLLAEEMHLARLYRGNGRMTMRFLGFLLAVGL
                     CIFFIVQKRFRRWNKAGLILASFVLMVVMAWRLLFPLLPPPQPTGTHDVVTEVAFYHY
                     QTERSEMATSGDTREIPVRVWRPKDAQAQHHPLFLYSPGSFGAEEANETLFLELASRG
                     YVVVGLNHPYHSFRSTLSDGRKIRVDSAFIKSVMRSQGSKDLPKTLAELRAWSNVRID
                     DINAVLDQVLDSQTGHHHEQCIDRKRIVLSGHSLGGSAALAVGRQRPEDVQALVILEA
                     PFASDIIGIDEDRYVFTDEWYPVPVLHIYSDALYARLDEITTYEMNVRLMKSGRPMYV
                     NKHISGVGHFGLTDMSLATPVLTYLLDGGLNTRPAPDALHELNGYVLEFLNAYNKEKC
                     VVLPQNDNHSSF"
     gene            complement(3643..4857)
                     /locus_tag="PLF67_06320"
     CDS             complement(3643..4857)
                     /locus_tag="PLF67_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE89663.1"
                     /translation="MFDRPLYYENIKPFIGKPFIKVITGIRRCGKSTMMLLIQQELIK
                     QGVDPRHIISINFESLQYGHLRSSRPLYEYVRELMPSIKGMAYVFFDEIQLVEDWEDA
                     VNSIMLDFSVDMYLTGSNSKLLSSEISSLLTGRFIHFTMQVLTYSEMLAFREGLSEAR
                     EDLLWLYIRRGGFPAIHLSPDFDERSSCMVLNSIFDSIVLRDVVQRYKIRDVELLRRI
                     LYFIADTVGSPVSAKRIADYFKSQARRVDMNTVHTYLDALVSSYIITRVQRYDIKGRE
                     ILKTREKYYLADSGLQHAVFGYRDRHISGILENIVHNELVSRGYVVYVGKLDALEVDF
                     IAEKAQQKVYVQVAYRLEGEQAVQREFAPLLKIKDSYPKFVVTMDPRFSDTIEGVRHI
                     PLERFLTDGRLF"
     gene            5047..6147
                     /locus_tag="PLF67_06325"
     CDS             5047..6147
                     /locus_tag="PLF67_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE89664.1"
                     /translation="MSRKPFLLGFGAMRFPQTEEGKIDRPLAAEMLRYAMEHGVTYYD
                     TAYTYHARESEPFLGEFLQNYDRASFQLATKLPQWLVESVDDARRIFEEQLERLKVDY
                     FDYYLVHSLRKKTYVKMVDLGVITYLEKEVQAGRIKQLGFSFHSSFEDFRYILHSRAW
                     DFCQIQYNWLDIEEQAGRAGYELATEHGIPVIVMEPIKGGSLLSIPPHLKEQVEQVAE
                     EDSIAALSLRWVAEHRNVPVILSGMSTLEQVVENIATLSDPQPLTDEQHSALEEVGSY
                     MRSRLGNGCTSCSYCMPCPFGVDIPGSFDIWNTFRLFGRYEQIRKRWESMGDKGPLSC
                     TRCMTCVSLCPQEIPIPFDLARVHEELSQGAL"
     gene            6164..7084
                     /locus_tag="PLF67_06330"
     CDS             6164..7084
                     /locus_tag="PLF67_06330"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOE89665.1"
                     /translation="MMEERIRLALLGWCLGDGFGSQTEGMEPERVEALGELAEVYSFE
                     KEVPHCGIGSYVSDLPILFALSFLELERVDYDHLRSTYRRYIKEEPDFLEPLLEQALS
                     NPMEKGERVFHLGRSVTSGILSALRSDSKMIAGKEAALTSANAIVREAADLYGQTLIS
                     LILREAETVDELFLLLMRSRTLDDELKSLIRGARNSEEVINPQAVNTLHLRPTLLAVF
                     SALQHATTFEDGMIALAKGGGSARLRCALYGGLAGVLFDHLPESWIAELHVSSALDAM
                     IKKQTLYRRETLTFEKIVDSLSSKLCDYEE"
     gene            7088..8059
                     /locus_tag="PLF67_06335"
     CDS             7088..8059
                     /locus_tag="PLF67_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE89666.1"
                     /translation="MKYKPLGRSGIMVSELCFGTMGFVSRTDRSSSERLYRKAREAGI
                     TFFDTANIYSDGEVEEYLGSFIRDERSEVVIATKGFGAMGSGPNDKGSNAKSLRLALE
                     GSLKRLKTDYVDIYFLHQYDPLVAEEELLRTLERFVTSGKVLSLGVSNYSAYQTMKLN
                     AAAARLGLPRIDVLQPMYSLAKRICEIEILPMAKGEGLGVISYSPLGAGLLTGRYSHE
                     DKPATGRLVENPMYTKRYLEERYFTLAKEFSAFAGSLGVEEATLATAWVLANEAITAP
                     IIGPTNEDHLRLSLKALDFEITKEIATRLDEIAPPPPPAHDRSEVDR"
     gene            8096..9511
                     /locus_tag="PLF67_06340"
     CDS             8096..9511
                     /locus_tag="PLF67_06340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOE89667.1"
                     /translation="MDDITSLARERFGINAVRPFQYLVIKRILEEDKKENPHPGMIVT
                     LPTGSGKSLCFMLPTLVVSGLIIIVYPLLALMSDQVRRFEEAKIPVLCLKGGQSKKER
                     MEILSDLANVRALVTNAETLAHPRVLSALLHIPISMIVIDEAHTIERWGSSFRPALRT
                     LSSLIAHLRPRQTLCFTATADNAVISLLNTLFFTGKPAHLVRGSSDRENIAYHSYPTL
                     SKSRAITTLLHNPSTRPAIVFAPTRALTERLARYCRISNPTLDARHYHAGLSKDARHM
                     IESWYFHHPDGVLFATNAYGMGMDKKDIRTVIHHSPPSDALAFLQESGRAGRDGLPAR
                     SIVLINEADRSSNLFPIFNQHKQCYRSLLLSLMDEELEHCFGCGVCLNHRILLREGER
                     PILRTIFFNPLKYTPYTLALTLQKKNARVLYSGSLSTWRIREIEEAVRTLVQEGRILQ
                     PPFLKGKMILLPQIDKRGRDR"
     gene            9540..10811
                     /locus_tag="PLF67_06345"
     CDS             9540..10811
                     /locus_tag="PLF67_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOE89668.1"
                     /translation="MATVREVQNGALHLRYPEFYGNVYDEAEHDRRYQTLIETHAKLF
                     SEENPILFSTAGRSELGGNHTDHNKGRVIAATINLDTIAAVTKRNDSIVTIISEGYPP
                     VTVDLNDLAVKKEEYNTTESLVRGIAFAFKERNLAIGGFCANTSSKVLKGSGLSSSAA
                     IEVLCGTIFNHLYNDDVLKPVDLAQIGQFAENTYFGKPSGLMDQLACAYGGIIGIDFK
                     HQVPKIQEVSCSFEEKGYRLVIVDTGGNHADLTHEYASVPIEMRAVAAFFDEENLRFV
                     SEERFLSRLPDLRSSLQNDRSILRALHFFEENKRVEKMLEALEADDMKRYLELVNESG
                     ESSFCFLQNLYPSIDPTEQGLSLAVALGKEILQGQGAVRVHGGGFAGTIQAYVPIALF
                     DRFTARMEKVFKAGSVTALAIRKAPTTALIY"
     gene            complement(10805..12073)
                     /gene="thrC"
                     /locus_tag="PLF67_06350"
     CDS             complement(10805..12073)
                     /gene="thrC"
                     /locus_tag="PLF67_06350"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOE89669.1"
                     /translation="MKFISTRGNAEPIGASQAIMQGLAPDGGLYIPESFPSLAHVNVH
                     EISSYPELAYEVLAPFFEGDPLAPHLAEICVNAFTFPAPLVQLEEGRFVLELFHGPTA
                     AFKDFGARFLAYSMEQLLQLQDRKLTILVATSGDTGGAVAAAFAGRKGIDVKVLFPKN
                     RVSARQQHQLTCWGGNIEAYEVDGSFDDCQAMVKAAFMDKEISKRWGLSSANSINLGR
                     LLPQMVYYVYGAVRVQAASGKKPHFIIPSGNVGNSCGAYWAKTMGAPIESITLALNAN
                     RTIVDYLKSGEYAPRASIETLANAMDVGSPSNMERLFALFGDLATMRLNVSAHSVDDP
                     AIRQTIKEVYEQHGYIICPHTATGVRVQKDVVSQSPSIVVSTAHPAKFETVVEPLIGH
                     VLDVPPALEEILKRETRMRSIGTDYLELFQ"
     gene            complement(12085..14439)
                     /gene="thrA"
                     /locus_tag="PLF67_06355"
     CDS             complement(12085..14439)
                     /gene="thrA"
                     /locus_tag="PLF67_06355"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOE89670.1"
                     /translation="MNLLRIHALEAHNVVTKAGLEKLLRILSPEEQSVLVISSLGNEA
                     LDVTDLLVLAKARDERLWSMAEARLSSWENMVESVLDVSNSVVATIKEGFDNIEDLLR
                     SVWLVGEVSDDVYFYVKKLSASWVAMIVHSFLQSVGIKSEYLEYSSVGKRVTHTVTVV
                     YCEEGEKRSEYAAALLASRLGANGVTFWNSTSLLKSADDGDVPSALVIRALSYAEATE
                     LSYYGSPIIHPQSLLPAIEAEIEVTLRCWNAEDDPGTVVSKEGNPNSPYKVKGFSTIR
                     NVALVNVEGVGMSGVVGISARLFSAIRKANVSVILISQGSSEYSICFAVYQDKMEEAV
                     RVARLEFKEELENKKIHSIEGHGNLAILAAVGQKMSGQRGIAGNFFSSLGRAGVNVVA
                     IAQGSSETNISAVILQQDVRRALRALHARFFLSRQTLSVGLLGPGSIGSELLSQIDAQ
                     LVRLREQFGLAIHIRGIANSKKMLLDDEGIDPGNWRDRFEKSSVDLDLTAFAAHISAT
                     YFPHSLIIDCSTSSELAGHYAGWLKNRIHVITPNKKAGTAEYSYYKELMDACLSTGSR
                     FLYETTVGAGLPVIWTLKDLVQTGDHVHRIEGMLSGTLAWLFSTYDGSVPFSTLVRQA
                     KEMGYTEPDPRDDLSGMDVGRKTVILARELGGKIEVADVPIQSLVPESLEGIGLDEFM
                     ERLEEIDPIIEQAYRTARSKGEFLRYVGTVDEEGNCEARLGSFGPDHPFSQATGTDNV
                     ILFVTDRYRTQPLVIKGPGAGREVTAGGVFSDILRLSSYLGARI"
     gene            complement(14508..14738)
                     /gene="rpmB"
                     /locus_tag="PLF67_06360"
     CDS             complement(14508..14738)
                     /gene="rpmB"
                     /locus_tag="PLF67_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOE89671.1"
                     /translation="MARRCELCGKGTVAGNSVPRKGQAKKKGGVGQHIGVTTKRTFRP
                     NLVTVKTIVKGTPKTIKICTRCLRSGRAEQLA"
     gene            complement(15340..15990)
                     /gene="nth"
                     /locus_tag="PLF67_06365"
     CDS             complement(15340..15990)
                     /gene="nth"
                     /locus_tag="PLF67_06365"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOE89672.1"
                     /translation="MNQAFEVYKRLDELSPPEIRFLDERDPFRFLISVILSAQTTDRI
                     VNIVTKDLFSKYPDAPSLAAADLKDVEEIVYPTGFYRNKAKNIIAASKALGDEAVPDT
                     MEELIKLPGVGRKTASCILGDIYDKPAIIVDTHFGRVVQRLGITAEKDPTAIELDVAG
                     QLEGRYHYRFSMIVNLFGRTTCHAKRPQCETCPLNDICPSAAFFLDQYAKKNRSSP"
     gene            16048..16120
                     /locus_tag="PLF67_06370"
     tRNA            16048..16120
                     /locus_tag="PLF67_06370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16081..16083,aa:Pro,seq:tgg)
     gene            complement(16264..18066)
                     /locus_tag="PLF67_06375"
     CDS             complement(16264..18066)
                     /locus_tag="PLF67_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89673.1"
                     /translation="MKTDGLTLYKKLVCSVLLFVVVLAAPLSADSLIYEAASGKLIGF
                     DVGVTDNPADPGLYDNNPEEVWRKSGFIGRIVYRGPATTFSFNNTGPTATGTQENRFY
                     FTRLLDPDSWKEFFLVVRLKGERHSGARIDFSGVNRVVEHPGGTITMPHGGGIEQVLI
                     DQEGYNANGEKGTYNGFNGYKYKYPYKFVWMDVTLIRTVNSSSLVPNSFYESNITVMA
                     ESGTSLSLLLSGEYGEPEDNHSASLSYDPVTGKLIGFDVGVTDSPSNPTAYDGKQNEV
                     WEKPGFIGRVAYTGPPTTLTFTNTGPVATGTQENRFYFTHLSNQGSWKEFFLVVRSKG
                     ITHSGTRIDYSEVNKVVEHPLDTITMPYGAGFEEVESGEEGYNIFGTKGTYNGFNGYK
                     YKYPYKYVWMDVTLIRTSNSLSLIPDSFYESNITVTATNGASLTLVLSGEYGDPTGYS
                     PSFYSFGVTKTVTDPFPISDLTGRKTPSQALKVGVVTYTSDNTAANIRFAANAAGTSE
                     VFLFTNENNNNIRFRYYLAFQATKPNRPLETVTPAKQFPTAVQTVYSPIDYPYRTYTW
                     NYLEGDLKIYLPDQVNPASGIYTSTVYCFVTPVH"
BASE COUNT         4177 a         5165 c         4832 g         4284 t
ORIGIN      
        1 ctactgcctt ggattcatac cctgccttgg atacatagag gctgtagatt ccgggttgta
       61 cacctgtaac cacgaatgca ccgtcctgtg ccgtagtcgc atcatattgg agctggttgt
      121 ccggggtctt cagcatcaca cttatgcccg tatgaacatt ggttccgatc aaagttacgc
      181 tgcccttcac agtcgccttg acactcttca agacaacagg gtctatcact gtcgtttccc
      241 catatgttgt atggaccagc ttgctcctgt ccgttacata gccgttcatc tgaatctgga
      301 tggaatactg cccgacatcc aggccgccta tgtagtaggt tccatcctca agtgtcgttg
      361 tagtgacgac cctgccttga tctcctacca acgttgcgct gaccgttact ccggaattgc
      421 cgggtgaacc ttccaggacg acacttcctg taattgcgca ggatgagctg atcaatatcg
      481 tttcaggaat gactgtcgga actccggccg acacggagac tccggtctca attacggtca
      541 cataacccat ctttgcaatg cttatctcgt agccttctcc aagcgggcag ttgttcagga
      601 catatgttcc ctccgcatcc gtggtggtgc tgaacgaata cctctttcca tcgattgtta
      661 tggtagccaa caccgccgca ccgcttaggg gttttccgtc gttcagggtg atcttgcctg
      721 ttatgtctcc tccattcgct gacaacgcaa ccgcttggcc tatgcttgtc gtctccgctg
      781 cccttaccgt aattccttca agtctttgcg gaacatgccc gggatatgag atcagggccg
      841 agtacgtgcc gggtttcaca tccggaataa ggaaggcgcc atcctcggta gtcgtgccgg
      901 ttgcaacgtc ttcaggatcg gccccttcca gccggatgat tatcccctca tgggactggg
      961 cgcctttcag ggtcacagtt cctcttacga ttcccgcctc ccgtatcagg cttgcctcgg
     1021 cgacaacctt cgtcgggctc tcatcatcgc tgtccgaggc aagaactaca ccttccttca
     1081 tcaagctcag atagccggag cttgtatatc ggattctgtg cgtcccctgc ggaacattcg
     1141 ttatgctgaa gttcccttct gcgtctgtca tcgccatgag tgatgttccc ggaatataga
     1201 ccatcacatc atagttgtcg gctgcaccgg ttctgatcgc cgtacccatt atggttccga
     1261 gcggcttcat cttgaccgac ccgacgcttg catctatgga tgcggacaca tcggtcagcc
     1321 atccgccata gggcttgcgt cctgccgccc tgtccccaac ggacctcttg ttctcgaatt
     1381 ccggctcgat gctggtgaag tggatattgt attttttctc ggaatcaaga ttgggtatca
     1441 ggaacgtacc atccgggttt acccggccta catattttgt ggtctctccc tcaaccgcct
     1501 tcacccagat ctcttcagga gccagctcgg atgaagcagg aagcgagatc ctgcccctga
     1561 ttcccggttt ctgtggcatc tgtatctcat ccgagcacga gataagcaga aaaaccgaca
     1621 ggacaatgat aaccgtgact atgggaagaa agcgcttatg cataagaccc acccttgaaa
     1681 aaagatgccg aagctcacca gcaacgagtt aagtataagt cacacaaaac cggaacacaa
     1741 gcaattaaac ttcaaccgac atcatctaac atattgcctg tcaagtcttt ccgagaagct
     1801 tgaagaagat atccaagccc attttccttg cgaaccgact tcagccagtt ccgatgtagt
     1861 ggaaacatct tctgactatc atcggtggaa aaaagctcac tgatactttg tatcaaggcc
     1921 aatatctgac ctattcaacc actaaatatc cggaatatcg caatatttta gaagcgttat
     1981 ttcctcctta atgcaaaaca tgaagcagac tcgctttcat attaacaaga gacctcttgt
     2041 taagagatca agagaatacc ttctaatagt atgcaaaaat agcccttaat gtagattttc
     2101 cagcactatt ttgaggtttt tctttgtcaa aaagtgaatg tacctatagt ttggttggtg
     2161 caacttcctg ttcatataca aatggaaaaa ttaaaacgta ttggaagcca gctactgcta
     2221 gaggagttca tcgaagcgct caccaaatgg gcgatggcga tcatcgtggc gcacaatcat
     2281 cccagtgggg tgctcgaact cagcgttgaa gaccgagagg agaccagacg attgaagaaa
     2341 gccggagata ttctcggcat cccgatcatt ctcggataag aacatgagat tattcataag
     2401 ataccgaaga tgaaatggat gatggttggc atgcctgcgg taccctaaaa cgatgagtgg
     2461 ttgtcgtttt ggggaagcac aacacacttc tctttgttgt aagcgttcag aaactcaagc
     2521 acatatccgt tcaattcatg gagagcgtcc ggagcgggac gggtgttcag tccgccatcc
     2581 agcagatacg tgagaacggg tgtcgccaga ctcatatcgg tcaatccgaa atggccgaca
     2641 cccgagatat gcttgttcac atacatcgga cgaccgctct tcatcaaccg cacgttcatt
     2701 tcatatgtcg tgatctcatc cagtcgggca tataaagcgt ccgaatagat atgcaggacc
     2761 ggcacaggat accactcatc cgtgaacaca taccgatctt catcgatacc gatgatatcc
     2821 gatgcaaacg gtgcctccaa gatcaccaat gcctgaacat cttccggtct ttgacgccca
     2881 acggccaacg ctgcagaacc gccaagggaa tgacccgaga ggacgatccg tttgcggtcg
     2941 atgcactgtt catgatgatg acccgtctgc gagtccaaaa cctgatcaag caccgcattg
     3001 atgtcatcaa tccgcacgtt cgaccatgct cgcaattcgg ctaaggtctt cggcaaatcc
     3061 ttggaacctt gtgaacgcat gacgctcttg atgaatgccg aatccaccct gatcttcctc
     3121 ccgtcagaga gcgtacttcg aaaggaatga taaggatggt ttaagcccac cacaacatag
     3181 cctcgtgacg ccagttccaa gaaaagcgtc tcattggctt cttcggcacc aaaagacccc
     3241 ggagaataga gaaaaagagg gtgatgttgg gcctgtgcat cctttggacg ccataccctg
     3301 acgggaattt cccgggtgtc accagaggtc gccatctccg aacgttcggt ttgatagtga
     3361 taaaatgcca cttcggttac gacatcatgc gttcccgtag gctgtggcgg cggtagcaag
     3421 ggaaagagca gtcgccaggc catgacgacc atcagaacga atgaagcaag aatcaagccc
     3481 gccttgttcc atcttcggaa tctcttttgg acgataaaga agatacagag accgacagca
     3541 agcaaaaacc ccaagaatcg catagtcatc cgtccattcc cccggtacag tctagcaaga
     3601 tgcatttctt ctgccaataa accctcattg gcggaggatg catcaaaaca gccgcccatc
     3661 cgtcaggaat cgttcaagag gaatatgcct gactccttca atcgtatcgg aaaagcgggg
     3721 atccatcgtg accacgaatt tgggatatga gtcttttatt ttcagcaagg gtgcgaactc
     3781 cctttgaacc gcttgttcgc cttcaaggcg atacgcaacc tggacataca ccttttgttg
     3841 agccttctcg gcgatgaaat cgacttccaa ggcatcgagc ttgccgacat acacgacata
     3901 cccgcggctg accagctcgt tgtgcacgat gttctccaga atccctgaaa tatgacggtc
     3961 acggtacccg aacacggcgt gctgaaggcc gctgtcggca aggtagtatt tttcccgggt
     4021 tttaagaatc tctcttccct tgatgtcgta gcgttggacc cgagtgatga tgtaggagct
     4081 taccaacgca tcaagatagg tgtgaaccgt attcatgtcg actcttcgag cctgactctt
     4141 gaaataatcg gcgatccgct tggcggaaac cgggcttccc accgtgtccg cgatgaaata
     4201 tagaattctt ctcagaagct cgacatccct gatcttatac cgttgaacca catcccgcag
     4261 gacgatcgaa tcaaaaatgc tgttgagaac catgcacgag gagcgctcat caaaatccgg
     4321 agacagatgg atggcgggaa agccgccccg cctgatatag agccaaagca ggtcttcccg
     4381 ggcttcggac aacccttccc tgaatgcaag catttcggaa tacgtcaaaa cctgcatcgt
     4441 gaaatggatg aaccgaccgg taagcagtga tgagatttcc gatgaaagca gtttcgagtt
     4501 cgacccggtc agatacatgt caaccgaaaa atccagcatg atcgaattga ccgcatcctc
     4561 ccaatcttca accagctgga tttcatcgaa gaatacgtac gccatccctt tgatcgaagg
     4621 catgagttct cttacatact catacaaggg ccgggaagaa cggagatgtc cgtactgcaa
     4681 gctctcgaaa ttgatggata tgatgtgccg gggatccacc ccctgcttga tgagctcctg
     4741 ctgaatcaag agcatcatcg tcgatttccc gcaccgacgg atccccgtga tgacttttat
     4801 gaacggcttt ccgatgaacg gctttatgtt ctcgtagtag agcggcctat cgaacatcat
     4861 ttccctccat attcaggatt atacccataa aaaggtacga agtaaagaaa gttattaggg
     4921 ttatacccat atatacttat tatttttata gagtttttcc ggttataccc ctattcccta
     4981 tccttctgaa ggtaaaagct ctttttttga atatatgcta cacttcctct tgatgttgga
     5041 ggctctatga gcaggaaacc gtttcttctg ggatttggcg cgatgcgctt tccacagacc
     5101 gaggaaggaa aaatcgatcg gccgctggct gccgagatgc ttcgctatgc gatggagcac
     5161 ggagtcacct attacgatac cgcctacacc taccatgccc gggagagcga accctttctc
     5221 ggcgagttcc tccagaacta tgaccgcgcc tcttttcagc tggcgacgaa gcttccccag
     5281 tggctggtcg aatccgttga cgacgcacga cggatctttg aagagcaact ggaacgactg
     5341 aaggtcgact actttgacta ctacctcgtc cattcgttaa gaaaaaagac ctatgtgaag
     5401 atggtcgact tgggagtcat cacctatctg gagaaagagg tacaggcggg cagaatcaaa
     5461 cagctcggct tttccttcca ttcaagcttc gaggacttcc gatacatcct tcattcaaga
     5521 gcctgggatt tctgccagat ccaatacaac tggctcgaca tcgaagaaca ggcgggccgg
     5581 gccgggtatg aactggcgac cgagcacgga atcccggtca tcgtgatgga accgatcaag
     5641 ggaggatcgc tcctttccat ccctccccat ctgaaagaac aggttgagca ggtggctgaa
     5701 gaggattcga tcgccgccct ctcccttcgg tgggtcgccg aacacaggaa cgtcccggtg
     5761 attctcagcg gcatgtccac gcttgagcag gtggttgaaa acatcgccac cctctccgat
     5821 cctcaaccgc ttaccgatga acagcattcc gccttggagg aagtcggctc ctatatgcga
     5881 agccggctcg gcaacggctg cacctcctgt tcctactgca tgccctgccc cttcggcgtc
     5941 gatattcccg gatccttcga catctggaac accttccgcc tgttcggtcg gtacgagcag
     6001 atcagaaagc gctgggaatc gatgggtgac aaaggaccgc tttcctgcac ccggtgcatg
     6061 acgtgcgtct cgctctgccc gcaggagatc ccgatcccct tcgatctggc ccgggttcac
     6121 gaggaactca gccagggggc cctgtaagag gtgcgcttga ccgatgatgg aagagaggat
     6181 ccgcttggcg ttgcttggat ggtgtctcgg ggacggcttc ggcagccaga ccgaggggat
     6241 ggaacccgag agggtcgaag cccttggaga actcgcggaa gtttacagct ttgaaaagga
     6301 agtcccgcat tgcgggatcg gaagctacgt ctccgacctt ccgatcctct tcgccctttc
     6361 ctttctggag ctggaacgcg tcgattacga ccatctccgc tcgacctaca ggcgctacat
     6421 caaggaagaa cccgatttcc tcgagccgct gcttgaacag gcgctctcga atccgatgga
     6481 gaaaggagag cgggtctttc atctgggccg atccgtcaca agcggcattc tctccgctct
     6541 tcgttccgac tccaagatga tcgcgggaaa agaagccgcc ctcacctcgg ccaatgcgat
     6601 cgtccgggaa gcggcagatc tctatggtca aacgctcatc tctctcatcc tccgggaagc
     6661 ggaaacggtc gacgagctct ttctcctttt gatgagaagc cggaccttgg acgacgagct
     6721 gaaatcgctt atccggggag cgcggaactc ggaggaggtc atcaacccgc aggcggtcaa
     6781 cactctgcac cttcgtccga cccttcttgc ggtcttctcc gccctgcagc atgccaccac
     6841 cttcgaggac gggatgatcg cccttgccaa gggcggcgga tcggcccgcc ttcgctgcgc
     6901 cttgtacgga gggcttgccg gcgtgctctt cgatcacctt cccgaatcct ggatcgccga
     6961 actgcacgtc tcttcggctc tggacgcgat gatcaagaag cagacgctct acaggcggga
     7021 gacgctcacc ttcgagaaaa ttgtcgattc cttaagcagc aagctgtgtg actatgagga
     7081 gtaacagatg aagtacaaac ctttgggaag aagcgggatc atggtaagcg aattgtgctt
     7141 cggcacgatg ggcttcgtca gccggacgga tcgctcaagc tccgagcggc tctacaggaa
     7201 agcccgggaa gcgggaatca ccttcttcga cacggcgaac atctattccg acggtgaagt
     7261 cgaggagtac ctcggctcct tcatccggga tgaacgctcg gaggtcgtca tcgcgacgaa
     7321 aggcttcggg gcgatgggaa gcggtcccaa tgacaagggc tccaacgcca agagcctgcg
     7381 ccttgctctg gaaggatcgc tgaagcgcct gaagaccgac tatgtcgaca tctacttcct
     7441 ccatcaatac gaccccctcg tcgccgagga ggagctgctg cgcaccctgg aacggttcgt
     7501 gacaagcggg aaggtcctca gcctcggcgt ctccaactac agcgcctatc agacgatgaa
     7561 gctcaacgcg gcagcggctc ggcttggttt gccccggatc gacgtcctgc agccgatgta
     7621 ctcgctcgca aagcggatct gcgagatcga aatcctgccg atggccaaag gcgaaggtct
     7681 gggcgtcatc tcctacagcc ccctcggagc ggggctctta accggtcgat actcccatga
     7741 ggataagcct gcaaccggcc ggctggtcga aaacccgatg tacacgaagc gctatctgga
     7801 ggaacgctac ttcaccttgg ccaaggagtt ttccgcattc gccggatccc tgggcgtcga
     7861 ggaggcgacg cttgccaccg catgggtcct ggccaacgag gcgattaccg ccccgatcat
     7921 cggaccgacg aacgaggatc acttgaggct aagcctcaaa gcgctcgact ttgagatcac
     7981 aaaagagatc gcaacgagac tggatgagat cgctcccccg ccgcccccgg cgcatgaccg
     8041 cagcgaggtc gacagatagc tgatgcgaac cggcaactga ttgcgaatac ggggtatgga
     8101 tgacatcacc tcccttgcgc gggagcgctt cggcatcaat gcggtgcggc ccttccaata
     8161 tctggtgatt aaacgaattc ttgaagagga caagaaggag aacccccacc ccgggatgat
     8221 cgtgacgctg cccaccggaa gcggaaagag cctttgtttc atgcttccca cattggtggt
     8281 ctcgggtctt atcatcatcg tctatcctct tttggcattg atgagtgatc aggtgcgaag
     8341 gttcgaggag gcgaagatcc ccgtcctctg cctcaaagga gggcagtcga aaaaggaacg
     8401 aatggagatt ctttccgatc ttgcgaacgt ccgcgccctt gtcaccaacg ctgaaacatt
     8461 ggcccatcct cgtgtgctat ccgccctgct gcacatcccc atctcgatga tcgtcatcga
     8521 tgaggcgcat acgatcgaac gctggggttc ctcgtttcgg ccggcgctga ggacgctcag
     8581 ctcccttatc gcccatcttc ggccgagaca gaccctgtgc ttcaccgcca ccgccgacaa
     8641 tgcggtcatc agcttattga acaccctttt tttcaccgga aagccggccc acctcgttcg
     8701 aggttcaagc gaccgggaaa acatcgccta ccattcctat ccaacccttt caaagtcccg
     8761 tgccatcact accctcctgc acaatccctc gacccggccg gcgatcgtct ttgccccgac
     8821 ccgtgctctg acggagcggc ttgcccggta ttgccggatt tccaatccga ccctcgatgc
     8881 ccgccattac catgccggcc tttcaaagga tgctcggcac atgattgaat catggtattt
     8941 ccaccatccc gacggggtgc tcttcgcgac gaacgcctac gggatgggga tggacaaaaa
     9001 ggatatccgg acggtgatcc atcattctcc cccctccgac gcccttgcgt ttctgcagga
     9061 aagcggacgc gcggggcggg atggcctgcc ggctcgatcg atcgttctga tcaatgaagc
     9121 cgaccgctct tcaaacctct ttccgatctt caaccaacac aaacaatgtt atcgatcgtt
     9181 gcttctttcg ctgatggatg aagagctcga gcactgcttc ggctgtggag tctgcttgaa
     9241 tcaccggatt cttctcaggg agggggaacg gccgattctg cgcaccattt tcttcaatcc
     9301 gctgaagtac accccctata cgcttgcctt gacgctgcaa aaaaagaatg cccgtgttct
     9361 ctattccggc tcgctttcca cctggcggat ccgggagatc gaggaagcgg tcaggaccct
     9421 tgtccaagaa ggaaggattc ttcaaccacc cttcctgaag gggaaaatga ttctcctgcc
     9481 tcagattgac aagagaggga gggaccgata gagtttttta caaacaaggg aggatcggca
     9541 tggcgacggt acgtgaagtc caaaacggtg cattgcacct gcggtatccg gaattctatg
     9601 ggaatgtgta tgacgaagcg gagcacgacc ggcggtatca gaccctgatc gaaacccatg
     9661 ccaagctgtt ttccgaagag aatccgatcc tcttctccac cgccggccgc agcgagctcg
     9721 gcggaaacca taccgaccac aacaaggggc gggtgatcgc cgccacgatc aatctcgaca
     9781 cgatcgccgc cgttacgaaa cgaaatgatt cgatcgttac catcatcagc gaagggtatc
     9841 ccccggtaac ggtggatctg aacgatctgg ccgtaaagaa agaggaatac aacaccaccg
     9901 agagtttggt tcggggtatt gcgtttgcgt tcaaggagcg caacttggcg atcggcggct
     9961 tttgcgccaa cacctcgagc aaggtgctga aaggttccgg cctctcctcc tcggcggcga
    10021 tcgaggtgct gtgcggaacg attttcaacc atctatacaa cgatgatgtg ttgaaaccgg
    10081 tcgacttggc gcagatcggg caattcgccg agaacaccta cttcggcaag ccttccggac
    10141 tgatggacca gcttgcctgc gcctatggag gaatcatcgg catcgacttc aagcatcaag
    10201 taccgaagat tcaggaggtt tcctgctcct ttgaagaaaa agggtaccgg ctggtcatcg
    10261 tggacacggg aggaaaccat gccgacctta cccacgagta cgcctccgtt ccgatcgaga
    10321 tgcgcgccgt cgccgccttc tttgacgaag aaaatctacg attcgtttcc gaggaacgct
    10381 ttctgtcccg gctgcccgat cttcgctcct cgttgcagaa cgatcgttcg atcctccggg
    10441 ccctccattt ctttgaagag aacaaacgag ttgaaaagat gctggaggct ctcgaagcgg
    10501 atgacatgaa gcgctatctg gaattggtga acgagagcgg tgaatcctcc ttctgtttcc
    10561 tgcagaacct ctacccttcg atcgatccca ccgagcaggg tctcagtttg gcggtcgccc
    10621 tcggcaagga gattcttcaa ggccaagggg cggtgcgcgt ccacgggggc gggttcgccg
    10681 gaacgattca ggcgtatgta ccgatcgctc tgttcgatcg gttcacagcg aggatggaga
    10741 aggtcttcaa agcaggcagc gtaaccgctc tggcgattcg gaaggcgccg accaccgcgc
    10801 tgatctactg aaacagctcg aggtagtcgg tcccgatcga tctcatgcgc gtctctcttt
    10861 tcaggatctc ttcaagagcg ggcggcacgt caagaacgtg cccgatgagg ggctcgacca
    10921 ccgtttcaaa cttcgccggg tgggcggtgc tcaccacgat cgaaggggat tgggaaacga
    10981 catctttctg aacccgcact ccggtggcgg tatgggggca gatgatgtac ccatgctgct
    11041 catacacttc cttgatcgtc tgccggatcg ccggatcgtc gacgctgtgg gcgctcacgt
    11101 tcaggcgcat cgtcgccagg tcgccgaaga gagcgaaaag ccgctccatg ttgctcggag
    11161 agccgacatc catcgcgttg gccagcgtct cgattgaagc gcggggggca tactcgccgc
    11221 tcttcaggta atcgacgatc gtccggttgg cgttcagggc gagggtgatc gattcgatcg
    11281 gagctcccat cgtcttcgcc cagtacgccc cgcagctgtt ccccacgttt ccgctgggga
    11341 tgatgaagtg gggcttcttg ccactcgccg cctgaacccg aacagcgccg tagacgtaat
    11401 agaccatctg agggagaagc cgtccgaggt tgatcgaatt ggcgctggac aggccccatc
    11461 gtttgctgat ctccttgtcc atgaaggcgg ctttgaccat cgcctgacaa tcgtcgaagg
    11521 aaccgtcgac ctcatacgct tcgatgtttc cgccccagca ggtcagctgg tgctgctggc
    11581 gcgcactcac ccgattcttc ggaaagagca ctttgacgtc gatccctttt cgaccggcga
    11641 acgccgcggc gaccgcaccg cccgtatcac cgctggtggc gaccaggatc gtgagcttcc
    11701 gatcctgaag ctggagcagc tgctccatcg agtacgctaa aaaccgcgcc ccgaaatcct
    11761 tgaacgccgc agtcggtccg tgaaacagtt caaggacgaa ccgcccttcc tccaattgaa
    11821 cgaggggtgc ggggaaggtg aacgcgttga cgcagatctc ggccagatgg ggagcaagcg
    11881 gatcgccttc gaagaagggg gcgagcactt cataggcgag ctcgggatag gaagagatct
    11941 catggacatt cacatgagca agagagggaa aggattcggg gatatagagc cccccatccg
    12001 gagccaagcc ctgcatgatc gcctgacttg ctccgatcgg ttcggcattg ccgcgcgttg
    12061 aaatgaactt catcgtccgt tctcctaaat ccgggcgccc agataggatg agaggcgaag
    12121 gatgtcgctg aagactcctc ctgcggtcac ttccctgccg gctcccggtc ctttgatcac
    12181 aagcggctgg gtccgatagc gatcggtgac gaaaaggatc acgttgtccg tccccgtcgc
    12241 ctggctgaag ggatggtccg gtccgaatga accgaggcgg gcttcgcagt tcccctcttc
    12301 atccaccgtt ccgacgtaac ggaggaactc tccctttgag cgagcggtcc ggtacgcttg
    12361 ttcgatgatc ggatcgatct cctccagccg ctccatgaac tcgtccaggc cgattccttc
    12421 aagggattcg ggaacgaggc tttgaatggg aacatcggcc acttcgatct tgccgcccag
    12481 ctcgcgggcg agaatcaccg tctttcgccc cacatccatc cccgaaagat cgtctcgagg
    12541 atcgggttcg gtgtatccca tctccttcgc ctgacggacc agggtcgaaa acggaaccga
    12601 tccgtcatag gtggagaaga gccaggcgag ggtaccgctg agcatccctt cgatccggtg
    12661 gacatgatca ccggtctgca ccagatcttt cagggtccag atgaccggaa gccccgcccc
    12721 caccgtcgtt tcatagagga accggctgcc ggtactgaga catgcatcca tcagctcctt
    12781 gtagtaggag tattcggcgg ttcccgcttt cttgttgggc gtgatgacat ggatgcggtt
    12841 tttcagccat ccggcgtagt ggccggccag ttcgctgctc gtcgagcagt cgatgatcaa
    12901 ggaatgcggg aagtaggtcg cgctgatatg ggcggcgaag gccgtcagat ccagatcgac
    12961 gctcgatttc tcgaagcgat ctcgccagtt gcccggatcg atcccttcat catcaaggag
    13021 catcttcttc gagttcgcga tcccgcggat atgaatcgcc aagccgaact gctcgcgaag
    13081 acggacaagc tgggcatcga tctgagaaag cagttcgctt ccgatcgaac ccggtccgag
    13141 caatccgacg gagagcgtct ggcgggaaag gaagaatcgg gcatggagag cacgaagagc
    13201 cctacgaaca tcctgttgga ggatgaccgc tgaaatattc gtctccgagg atccctgagc
    13261 gatcgcaacg acgtttactc ccgccctccc caaggaggag aagaaattcc cggcgatgcc
    13321 gcgctggccg ctcatcttct gtcccacggc ggcaagaatc gccagatttc cgtggccttc
    13381 aatgctgtgg attttcttat tctcgagttc ttccttgaac tcgagccggg cgacgcggac
    13441 cgcttcttcc atcttgtcct ggtagacggc gaaacagatc gagtattcgc tggatccctg
    13501 actgatcagg atgacggaaa cattcgcctt gcggatcgcg ctgaacaggc gggcggagat
    13561 tcccaccacc ccgctcattc ccacaccttc gacgttcacc agggcgacgt ttcggatcgt
    13621 tgaaaatcct ttcaccttgt acggactgtt cggattgccc tctttgctga cgaccgttcc
    13681 gggatcatcc tcggcgttcc aacagcggag cgtcacttcg atctccgctt caatggcggg
    13741 cagaagggac tgcgggtgga tgatcggcga tccatagtag gaaagttcgg tcgcttcggc
    13801 atacgagagg gctcgaatga cgagtgcgga aggaacatcg ccgtcatcgg cgcttttcag
    13861 aagcgacgtg gaattccaga aggttactcc gttcgctccg aggcgcgagg ccagaagcgc
    13921 tgctgcatat tcgcttctct tctctccttc ttcgcaatag accacggtaa ccgtgtgtgt
    13981 aacccgcttt ccaacgctgc tgtattccag atattcactt ttaatcccga cgctctgaag
    14041 gaaggaatga acgatcatcg ccacccacga ggcggagagc ttcttgacgt agaagtatac
    14101 gtcgtcgctg acctccccga cgagccacac cgatcgcagg agatcttcaa tattgtcgaa
    14161 cccctcttta atggtggcta ctacggagtt gctcacatcc aagacggatt cgaccatgtt
    14221 ttcccaagag ctcagtcggg cttcggccat tgaccacagg cgttcatccc gggctttggc
    14281 gagaaccaga agatctgtta catccaacgc ctcattgccc aaagaggaaa tgacgaggac
    14341 gctttgttct tcgggtgaaa ggatccgaag aagcttttcc aagcccgctt ttgtaacgac
    14401 gttgtgagcc tccaaggcat ggattcgtag cagattcacg acaatttacc ctccatatac
    14461 tgaaacagag cccaaagggc cctgttgcaa ttgcgctgac gtactctcta tgcgagttgt
    14521 tcggcccgtc cacttctcaa gcaacgagtg caaatcttga tcgtcttggg tgttcccttg
    14581 acgattgttt tcactgtcac gaggttgggg cggaacgtgc gcttggtggt aacgccgata
    14641 tgctgaccga caccaccctt ctttttggct tgacctttac gcgggacgct gtttcctgca
    14701 acagtccctt ttccacacaa ttcacatctg cgagccattg ctatatccac cttctctaca
    14761 agttcaacaa gttatcggag tctcatcatc tttgtccggg aaaacggctt cccgtgagca
    14821 gtgaaacaaa gcccaactcc aacctaggca aacatatcaa attcccgaat atgtgtaaag
    14881 agagcgggag gatttgtgtt gttgttcagc gaaaacgata cctgattagg agttctcctc
    14941 atgcagaaac tccaggaatc atctatactg caggtatgca ggactattat atccttcgtc
    15001 ttcacaaaga tcttcgcatc gctttggaaa aagaacggaa tcgactctat gcgctgtgtg
    15061 gcgatcgttc gcttttggtc tgggaaccat gcatcatctt gggacccgct tccgaccagg
    15121 ctgcccatat cattccatcc cctccgcttc cggtcatcgt caacggcacg gcccgttata
    15181 ccaacggcat tctccacctc ccgttagctg attcgacggc tttggaccga acccgcgagt
    15241 cgcttcaaac ttcgtggccg attcacggca ttttcctggg caccgtggat attgagtacg
    15301 aacgggcgga gctcgccttg cgctccctga gcttcgcggt tatggagacg accgattctt
    15361 cttggcgtat tggtcgagaa agaaggctgc actcggacat atatcgttga gcggacaggt
    15421 ctcgcactgc ggccgctttg cgtggcaggt cgttcgccca aagaggttga cgatcatgct
    15481 gaaacggtag tggtacctcc cctccaattg ccccgccaca tccagctcga tggccgtcgg
    15541 atccttctct gcggtgattc ccagccgttg aacaacccgg ccgaagtgcg tatccacgat
    15601 gatcgccggc ttgtcgtaga tatcccccag gatgcaggaa gcggttttgc gccccacccc
    15661 cggtagttta atcagctctt ccatcgtgtc gggcacagct tcatcgccga gcgctttact
    15721 ggcggcgatg atgttcttcg ccttgtttcg gtaaaagccg gtgggataga cgatctcttc
    15781 cacatccttg aggtccgccg ctgccagcga cggggcatcg ggatatttgg aaaagaggtc
    15841 tttggtaaca atattgacga tgcggtcggt cgtctgggcg gacagaatga cgctgatcag
    15901 aaagcgaaaa ggatcccgct catccaggaa tcggatctcg ggcggcgata gttcatcgag
    15961 tcttttatat acttcaaacg cttgattcat ggcttcatcc taccccttga cggaaatgag
    16021 agcaactctc tatactcaaa acgcattcgg ggcgtagcct agtggctatg gcgcctgctt
    16081 tgggagcagg atatcgaagg ttcgagtcct ttcgccccga cctattgtat cttgtttatt
    16141 ccatcaagat gcttggcaac ttgtttgtag atacaacatg caaacaggtt gccaattatt
    16201 attatcttta tctgcatcca tcttcgcttc aagcagatta ccgacctgct ccacagttgg
    16261 gtatcaatga acaggggtta cgaaacagta gacggtggac gtatagatgc cgctcgcagg
    16321 gttgacctga tcgggaagat agatcttcaa atccccctcg aggtagttcc acgtataggt
    16381 tcgatacgga tagtcgattg gcgaatacac cgtctgcact gccgttggaa attgttttgc
    16441 aggagtgacc gtctcaagag gacggtttgg ctttgtcgcc tggaaggcga ggtaatagcg
    16501 aaacctgatg ttattgttat tttcgttcgt gaaaagaaat acttcggagg ttccggcggc
    16561 attggcggca aaacggatgt tcgcggcggt gttgtccgat gtataggtta cgacgccgac
    16621 cttcaacgcc tgggagggcg tcttcctgcc ggtcagatcg gaaatgggga agggatcggt
    16681 gaccgtcttc gttacgccga atgagtagaa cgaaggtgaa tagcccgtgg ggtctccata
    16741 ctcgccgctc agaaccagag tgagactggc tccattagtc gctgtcacgg tgatgttcga
    16801 ctcatagaag gaatccggta tcaaggacaa actgttggag gttctgatca gcgtcacgtc
    16861 catccagacg tatttgtagg gatatttgta tttgtagccg ttaaagccgt tgtacgtacc
    16921 cttggtgccg aagatattgt acccttcctc ccccgactcg acttcctcaa atccggcacc
    16981 ataaggcatc gtgatcgtat ccaagggatg ctccacgacc ttgttgacct cggaatagtc
    17041 gattcgggtg ccggaatgcg tgattccctt cgacctgacc acgagaaaga attccttcca
    17101 gctgccttga ttcgacaggt gggtgaaata aaaccggttc tcttgggtgc cggtggcaac
    17161 cggtccggta ttggtaaacg taagggtcgt cggcggtccg gtgtaggcga cccttccaat
    17221 gaagcctggt ttttcccaca cctcattctg cttgccgtca tatgcagtag gattggaggg
    17281 ggagtcggtc acaccgacat caaaaccgat gagcttcccg gttaccggat catacgacag
    17341 ggaggcggaa tgattgtcct cgggctctcc gtactcgcca ctcagaagca gggagagact
    17401 ggtgccgctt tccgccatca cggtgatgtt cgactcataa aaggaattcg gtaccaagga
    17461 cgaactgttg acggttcgaa tcagcgtcac gtccatccag acgaatttgt agggatattt
    17521 gtatttgtag ccgttaaagc cgttgtacgt acccttttcg ccgttggcat tgtacccttc
    17581 ctgatctatc aaaacttgct cgattccacc gccatgaggc atcgtgatcg tcccaccggg
    17641 atgctccacg accctgttga ctccggaaaa gtcgattcgg gcgccggaat gcctttctcc
    17701 cttcaatctg accacgagaa agaattcctt ccagctgtca ggatccaata ggcgggtgaa
    17761 ataaaaccgg ttctcttggg tgccggtggc tgtcggtccg gtattgttga acgaaaacgt
    17821 cgttgccggt cctctgtaga cgattcttcc gatgaagcct gatttcctcc acacctcctc
    17881 cgggttgttg tcatacaatc ccggatcggc gggattgtcg gtcacaccga cgtcgaaacc
    17941 gatgagcttc ccgcttgccg cttcatagat cagggagtcg gcagagagtg gtgctgccaa
    18001 tacgactacg aaaagaagaa cggaacatac cagtttttta tacaatgtca gaccatctgt
    18061 tttcaccgtt gctgtaacct catgatgctt tatctctatg gacaatccgg catatatgta
    18121 taacctacag acattccgtc atcgatgtca acccaagatt tctcttggtt atgtatattg
    18181 ccgttagagt tgtttatctt catgtgtaga cccgtcatga tgatgaaacc tttcccgata
    18241 ttgttgcgtt gagttttttt ccggcaccat gggattggat attggggtgc aagaatgcgg
    18301 tggccggcag gcctccatgc cgggcgttca gcattcagga attcccggat gattggggtg
    18361 atcaccggct cgttgaagga cgaacacaaa taaatcggaa tccgagcatt tcccgttctc
    18421 acgatacgtc aacaccgact ccggccggat cgggtgtc
//
LOCUS       DAOSLI010000037        18410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3253_length_18410_cov_29.715010, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000037 DAOSLI010000000
VERSION     DAOSLI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3253_length_18410_cov_29.715010"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..723
                     /gene="pyrH"
                     /locus_tag="PLF67_06380"
     CDS             40..723
                     /gene="pyrH"
                     /locus_tag="PLF67_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013758595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOE89674.1"
                     /translation="MVTVISLGGSIIVPDSVDVAFLGRFNEAVRTYLAQDEKRKLILV
                     CGGGAPARVYQQAMKSLDEDTPSEALDWLGIKATHLNAQLVKTLFTDLAPNEVVTDPT
                     APFTFEGRILVAAGWKPGFSTDTDAVYLAERFDSKLIINLSNTAQVYTADPKKDPDAK
                     PLVNVSWKEYRRMIGNEWTPGRSTPFDPVASARAQELGMSVVCADGRNIDNTIAILEG
                     REFFGTTIA"
     gene            complement(710..1645)
                     /locus_tag="PLF67_06385"
     CDS             complement(710..1645)
                     /locus_tag="PLF67_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE89675.1"
                     /translation="MSPYEACALCPHRCLVNRLEGERGVCNESSTVRIAWAGLHRGEE
                     PPISGEVGSGMIFFSGCPLHCAYCQNRQISSRNGYGMEIGIEELSTLMLALQGMGTKT
                     LNLVTGTHFIPSIVEALIGAKQKGLTLDVVWNSSGFESIEGLSLIDPSIDLYLIDLKT
                     LDEGVSSRFCGSVRYQRSILRVYDYLIKKGRNTFIDEDGLLKGVLVRHLLFPGEPAAT
                     EEVLRYFADHLKESMHLSLMVQFEDPQGMNRFSKVSQEEYDRLLELLEELEIENGFVQ
                     EIEDSISWIPDFTQDEPFPPTFSEVCPYFLELKRS"
     gene            1790..2938
                     /locus_tag="PLF67_06390"
     CDS             1790..2938
                     /locus_tag="PLF67_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE89676.1"
                     /translation="MPWPFLPSSKPKPHEEHRFILSIDGGGMRGIVPAAILGRLSHQL
                     EVLGSNIPLYAYFDLIAGTSTGGLLALGLSVPASKSNLPGDGKDAIPVYEDIKGKWLF
                     GGKPKQRYVGTIPPSIDLSALEELYFERGSTIFPKSQTRRIGQMFIDKYDEQPLERFL
                     FEMFGNTPLDEAMVPTLIVAYDSDEGQPFYLTSYESGSFLYWEAGRATSAAPTFFRPA
                     VLYDRQKQRTVRLIDGGVIANNPSLFAYQEAKKLYPDATRFHILSLSTAGINYTFKTD
                     GAAGTGVIGWIDPTKGAPIQKIYANAQLQVVDSIAEAIPDLTYTRVHGEARRDYKMDE
                     TDPRMLKDLRFDAEEIYAKNEEKILTFLTALVKRTTFDHVRLEERANE"
     gene            2931..3338
                     /locus_tag="PLF67_06395"
     CDS             2931..3338
                     /locus_tag="PLF67_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89677.1"
                     /translation="MSSHPKQLALEQTLLELSHAVDDYLEDRYHDYFIRHPNRPKRGS
                     TASASHDGLFSIGTQFTAGYGSDLGRGYIVVIEIRTLQNVPSVIRRKIEEDGIEYLRT
                     QLPVFFPDRKIEVKKDGNLYKLVGDFSLGSVGH"
     gene            complement(3465..4313)
                     /locus_tag="PLF67_06400"
     CDS             complement(3465..4313)
                     /locus_tag="PLF67_06400"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOE89678.1"
                     /translation="MQKTCVAIHDLASYAKSSLTVVLPALEALGVEVSPLPTALLSSQ
                     SDGFDSYFFQPLTKEMRSIIAEWQKLDLAFDSIYAGFLGSAEQVSLITEFIHFHRAKK
                     NPPLVLVDPVMGDDGELYGPMEKEHIDAMKKLVTEADCICPNVTEAALLLGYPYRERF
                     DEQTILQWAKELAYQSKANVAITSVPVEGKMAVACTEKDGSYLVYYDQIDTSYPGCGD
                     LFASILLAYLLDKDPFRLSVTKAAQLTALAIKKTQNLGYPYRHGVSVTSITGELALNR
                     IESKQS"
     gene            4369..6216
                     /locus_tag="PLF67_06405"
     CDS             4369..6216
                     /locus_tag="PLF67_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HOE89679.1"
                     /translation="MIIHDTLNDQAIADWRTNPLAFPLPSKESLSDAYTSMIISSSGW
                     RKVFAPGGDEEDSGRTVNPPDRILAALAAYALYLHVGKKKPTILVGLDARPTGAHLGS
                     IAVYTLLSLGAKVRYLFITAAPQIMADTNLHPKDADAFLYISASHNPIGHNGFKFGKE
                     GGVYGSKDAERLAERFCELVGDGLTHFTLLQTYAENLDPQIYKRTLERVGEEREKSAE
                     RYRTLALRIATKSDDEQAHTRFIETMRIEHQQKPLGILGELNGSARSCSIDRPLLSEL
                     GITTEFMHDTPGDVVHPIVPEGKNLTLCMESLTALHQKNPAFVLGYVPDNDGDRGNIV
                     YINDRKTATILEAQSLFALIVLSELAQSRLSDPDAPLAVVVNGPTSNRVDEIARSFGA
                     KVFRAEVGEANVVELSNLKRKEGYVVPILGEGSNGGNISHPAKVRDPLNTLLSLTKLL
                     KQEELSRLWFTLSGQPQPASLTLDQLICSLPSYITTGAYSSLAQLSVNIEHGRLKTRY
                     EEFFEEIFNRDHILFNSFGITSYKIIQTEGTEERIGSGPAYRTPPYNGGFKVGLYNDE
                     NVMTDFLWMRGSKTEPIFRLLVDCKGDNTNRHDYLLELHRLVITRALQS"
     gene            complement(6217..7530)
                     /locus_tag="PLF67_06410"
     CDS             complement(6217..7530)
                     /locus_tag="PLF67_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexokinase"
                     /protein_id="HOE89680.1"
                     /translation="MHELIKKTEEFLNRSKIDADQVDMDQLITSFLSEMEKGLAGAPD
                     SSLKMIPTYTTTSDTILADQPVIVIDAGGTNLRTCLVRFDREKNPRISEFKKTSMPGV
                     KSEVTAKEFFSIIADEVERLIDRSDRIGFCFSYAAEITSDRDGIPLVFSKEIKAPEVI
                     GMPLGRSLLAELASRGHDVRKKKVAVLNDTVATLLAGQGTPSDVPYSGYIGFILGTGT
                     NTAYVESNKKITKLSADPSKAQIINIESGNFAYCPGPFDRKFFDSTKEPNQYHLEKMI
                     SGAYLGPICGSIITEAADAGLFSETFKERYAKRIKTLSTTVMSNYLEMPFNRDYELVQ
                     CVEGNEGDARVLWLIINAVIERAAKLTAANLTSTILKSGSGTDPRKPVCINADGTTLY
                     KTEYLLEYTNFYLHHYLQRKYKRYYRFVRIDDSPTIGSAVAALGL"
     gene            complement(7534..8145)
                     /locus_tag="PLF67_06415"
     CDS             complement(7534..8145)
                     /locus_tag="PLF67_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89681.1"
                     /translation="MNRQTIGLVLILLLIIAPLTAAKPSERDILIAVTAISDATIANV
                     AAYLNTPALNLPGSIFEKEARATLPKALELKDADLGIYRKTYQSLNKPQSNFLLSLLQ
                     SAKGPLNDVALLFLDTHEWEEGQVSLTGRVSTVWGEGVTLASLMTSVVTGGAINPIEA
                     IVDVKAAGTRLSTDVSISGSFLLFTDQEGYFVIEPRELKVNGE"
     gene            8212..9624
                     /gene="sbcB"
                     /locus_tag="PLF67_06420"
     CDS             8212..9624
                     /gene="sbcB"
                     /locus_tag="PLF67_06420"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HOE89682.1"
                     /translation="MIKPSILWYDLETFGLDSRGDRIAQFAAIRTDLDLRQIEEPIIL
                     YCKLSDDYLPDPLSCLVTGITPQEVNAKGLVEAEFIQLINTEFSRPDTTVAGFNSIRF
                     DDEFIRNALYRNFFDPYRREYEHNNSRWDIIDLVRATHDLRPEGIRWPERNEVNRPVF
                     RLTSLTEANGIEHLGAHDALADVRATIAIARLIKEKQPRLYQYAYDLRSKNRVKRLLK
                     APFGGEHTSLILHTNPLFSSEYGNTRLILPITYLKTNANAVVCFDLSCEVEPLLSATE
                     ETLMNTPGVFTISINKCPFVSPVNVLTEELERKLGIDSALAATKAAYIRTHPNVIAVI
                     HRVKDTFERVDDVDYQLYDAFFTNRDQQVFETLRQAKPREKMGFLNQVQDPRFGTLLF
                     RHVGRNWPEAFTEEERKRWISHAANRTLNPPGDGKMTWKFFYRSIEEKLESVDTTPRE
                     KQLLAELKEYGTALEAKIFG"
     gene            9669..10733
                     /gene="prfA"
                     /locus_tag="PLF67_06425"
     CDS             9669..10733
                     /gene="prfA"
                     /locus_tag="PLF67_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOE89683.1"
                     /translation="MLEKLAEYQSQLEQIDLQLADGATMRDMNLYKKLTQERSHLQPI
                     VDELSRLQEIADQIADAKELLKEETDTEMIALTREELENLEAALEKTTSKVKLLLIPP
                     DPLAGKDIIMEIRAGTGGEEAALFAADLFRMYSHYADSRSWKMDILSSNETGIGGYKE
                     LIVSISGKDVYASLQYESGVHRVQRVPETESGGRIHTSAVTVAVLAEAEETDIEIRTE
                     DLKVETMRSSGPGGQSVNTTDSAVRMTHLPTGLVVVCQDEKSQIKNRAKALRVLRSRL
                     FEMEEEKKAKERAEDRKSQVGSGDRSERIRTYNFPQNRLTDHRINLTLYKLDLIMAGN
                     LDEVIEPLKIASGEAALAEA"
     gene            10735..11568
                     /gene="prmC"
                     /locus_tag="PLF67_06430"
     CDS             10735..11568
                     /gene="prmC"
                     /locus_tag="PLF67_06430"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOE89684.1"
                     /translation="MTLNAWIAETKNLFIEQGVSDTPGLDARLLLGHATGYDQGRQIV
                     NAGEELAIHQLDLLENLRSQRLAGRPIAYIIGSKEFYGRSFYVDERVLIPRPDTEILV
                     ETVLKAIEDGVVSSEDSIIDVGTGSGAVGITLAAELDCMVLLSDISCDALDVARINSR
                     RILGRELETIASDLLPLGRKYGIIVSNPPYLTDEWVAAVDQNVRSEPDLALRGFGEDG
                     LDIIRALVAESSNHGSLFIECDYRQTKQVAGLFEEHGFTNISIQRDLQGLERVVWGVR
                     T"
     gene            11565..13601
                     /locus_tag="PLF67_06435"
     CDS             11565..13601
                     /locus_tag="PLF67_06435"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOE89685.1"
                     /translation="MNAQLIERFIQKAFKYPQEDQEKILAAATFADSKHDGQKRASGE
                     PYIIHPLAVGEILIQLKMDADTISAGVLHDTLEDTDTTYEELSRLFGKAVADMVEGET
                     KIASIKTMSKSVQEAETIRKMFFAMSEDIRVIIIKLADKLHNMRTLHHLKNERAREIA
                     NDTLDIYAPLADRLGISWMKDELEDLSLKTLKPDTYNHIQEYLLSKKSEQTAYLTRVE
                     KSIYRACGDANIADVVVTSRAKHAYSVYMKMKKRKKEIDEIFDILGVRILCTSVIDCY
                     TILGVVHRLWPPIEGRFKDYIAMPKANNYQSLHTTVMALDGKLLEIQIRTHEMHFTAE
                     YGVAAHWSYKAETGSESGNWAQMDDEQFTKIVSKLKAWSSEIENSESYMDDIKGDLLR
                     DTIYVFTPKGNIVELPKNATALDFAYQIHTEVGNRTTGAKADGSIIPLNAPLKNTQVI
                     EILTSPNARPHLNWLRYAQTNSARKKIRAWLNKYDENILIDKQIIAKRKEIEQAQAKI
                     EKRPPEQQVEGDDDEIIRQHVDPSRMKFRVGDEKNMMIHIAQCCNPVRGDEIIGYVSR
                     GRGIIVHRTDCPNLKNMAEVKDRSIDVEWETEFPKLTKRFAVTSKRTYDLFGEIEGAL
                     RKYKGHLIEGRLQDDEDGKLMGTFTMEVQQEDDFKKIIKTLKTIPSINTISEIR"
     gene            complement(13603..15237)
                     /locus_tag="PLF67_06440"
     CDS             complement(13603..15237)
                     /locus_tag="PLF67_06440"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOE89686.1"
                     /translation="MAKIFVSGHKNPDMDSICSAYAYAALKNKVDPSNTYIPIRCGNL
                     NEATRAQFNRFGLIPPPFKRDVRTRVSKVIIESDVEVCPTDPVYRLITLYKNTARHSV
                     FPIMEGTRFVGLVTIDEISDFILKENLGSRPVYNFLVKNFEVVIPGYFIKKGNEESIL
                     APLAVGANKFATYEKVMKGFKGPMPILVVGDRERHINKAIELNVPAIILTGIRGKTIS
                     KIDWDSYEGSVFVSETDTAETLRLLRLCVPVSELLVKKLPSLQADMLFDEARAFLANN
                     DYRELPVFSGKEYRGYVSRRSFLEKPATKLIMVDHNENDQAITGVEEAEVVEIVDHHR
                     LGAAKTRNPIFICCEPLGSTCTIVYKLFMRHNVEVTSDIAKVLLSGIVSDTIMLKSPT
                     TTFEDYTAVQDLLSIAGVDDMYKFGETMFSGGASLAKSDARMMIEADFKRYRESGVNF
                     GIGQSEVTTLDDVEDYRARYLEELEMVKKAYSLDWALFLITDVVKENSVLLLTRMPIA
                     EQKLAYEKAGEGMYLLPQVLSRKKQLLPEIIRVIQE"
     gene            15403..15849
                     /gene="rpiB"
                     /locus_tag="PLF67_06445"
     CDS             15403..15849
                     /gene="rpiB"
                     /locus_tag="PLF67_06445"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOE89687.1"
                     /translation="MPKVVIANDHGAVELAVTLTEYLKKMGYEVNHLGTTTEDSVDYP
                     DMALAATTEFKKGGYEFGILLCGTGIGVSISANKVDGIRCALVHNTYTAQMAKRHNDA
                     NFIAFGGRVVYREEVEDILDAFIDATFEGERHQRRIDKIMALENRN"
     gene            complement(15846..17009)
                     /locus_tag="PLF67_06450"
     CDS             complement(15846..17009)
                     /locus_tag="PLF67_06450"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOE89688.1"
                     /translation="MIEAILFDMDGVLIDSEPIILKAAMRLFADKGVTVKKEDFTPFI
                     GTGDKRYLLGVGEKYGLDLDFEVEKLVLFSHYARIAKESGPLGGVIRFITNAKRAGMK
                     IALVTSAVRMKMLLNLEAVGVEESIFDKVVTGDMVKRNKPHADIYQIASLLLGIDSKK
                     CLVVEDALNGTVAGKAAGCTVLGLTTTFGREELFESGADAVMGTLDEFDDFSTSNEFN
                     ELLRGYVGVHGGTPFGANLIADPVKEMDEGVLREAIAAALKARLNAYAPYSNYQVGSA
                     IVSHRTKGVYPGANVENSSYGATICAERSALLRAIADEGVVGIECLVVVSSDSPPAPP
                     CAQCLQVLAEFSRSDTAVHLLDVDAAEGRDGVHLVRRFDELLPHPFIFPSRRF"
     gene            complement(17009..>18410)
                     /locus_tag="PLF67_06455"
     CDS             complement(17009..>18410)
                     /locus_tag="PLF67_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE89689.1"
                     /translation="MKRLLVTQGLLVDTEWTRHADILIEDGIIVRIAPKIEVGEIDGP
                     VEIVRADGLAILPGIIDAHTHYHLVSRNTVTADSFAEGSRAAAYGGVTTVVDFADDDK
                     KRSLLVNAKERIAAMGKEMAIDFSLHQGIYSYRPSLKEELLELKEAGVKVIKLFTTYK
                     NVGYMIEKEEELKKIFAICKELDLLVTIHAEDDEVIQRAEKAYKGDYAPASHPILRPS
                     EAEAVGIRTMGKIALEAGIPIYIVHLSSKAGLEAVRDLRRQGLRVIVETTPHYLFLTG
                     EKLAGADGALWVMTPPLRDEEDRLALQEALLNGEIQVVATDHCAFTKEQKLSSKDSRT
                     ILPGIPGSEELLSLVYNFAAKSGRIGLQQVVNLLSTAPAKAFGLYPRKGALRIGSDAD
                     FVLFDPDHQWKIGDASIHSASGYSAYAGMTVVGKALMTYLGGRLIMGDGVYLGLAGGG
                     EFIPQHSVGRGKTVMH"
BASE COUNT         4368 a         5189 c         4889 g         3964 t
ORIGIN      
        1 attccaattc tgctagagtg aaaaccggag gaaatgaaga tggtgacggt tatttcactc
       61 gggggctcga tcatcgtccc cgactcggtc gatgttgcgt ttcttggtcg gttcaatgag
      121 gctgtccgga cctatctggc tcaggatgaa aagcggaagc tgattctcgt ctgcggcggc
      181 ggggcacccg ctcgagtcta tcagcaggcg atgaaatccc tcgatgaaga cactccgagc
      241 gaagcgcttg attggctcgg catcaaagcc acccacctca acgcccagct ggtgaaaacc
      301 ctcttcacag acctggctcc caacgaggtg gtaaccgacc ccaccgctcc attcacgttt
      361 gaagggcgaa tcctcgtcgc cgccggatgg aagcccgggt tttccaccga cacggatgcg
      421 gtctacctcg ccgagcgctt tgattccaag cttatcatca acctctccaa caccgcccaa
      481 gtctacaccg ccgatccgaa aaaggatccc gatgccaaac cgctggtaaa tgtctcatgg
      541 aaagagtacc ggagaatgat cggaaacgaa tggacacccg gccgctccac ccccttcgac
      601 ccggtcgcct ccgcccgggc ccaggagctc ggcatgagcg tggtctgcgc ggacggacgg
      661 aacatcgaca acacgattgc gatcctcgag ggcagggagt tcttcggaac tacgatcgct
      721 tgagttccaa aaagtacggg cacacctcgc tgaaggtcgg gggaaacggc tcgtcctgag
      781 tgaaatcggg aatccacgag atggaatcct cgatctcctg aacaaagccg ttctcgatct
      841 ccaactcctc cagcagttca agcagtcgat catactcctc ctgagaaacc ttggaaaatc
      901 gattcatccc ttgtggatcc tcgaattgga ccatcagact cagatgcatg ctttctttaa
      961 gatgatccgc aaagtatcga aggacctcct ccgtcgctgc cggttcaccg ggaaagagca
     1021 ggtgccgaac cagcacaccc ttgagaagcc catcttcatc gatgaaggtg ttccttcctt
     1081 ttttgataag atagtcatac acccgaagaa tcgagcgctg atagcggacg ctgccgcaga
     1141 atcggctgct gaccccttca tcgagggttt tcaggtcgat aagataaaga tcgatagagg
     1201 ggtcaatcag ggacagccct tcaatcgatt cgaaccccga ggagttccag accacatcca
     1261 acgtcaatcc cttttgcttc gccccgatga gcgcctcgac gatcgatggg atgaaatggg
     1321 ttcccgttac cagattgagg gtcttcgtcc ccattccttg gagggcgagc atcagggtgc
     1381 tcaactcctc gatgccgatt tccattccgt agccgtttcg ggaagagatc tgacggttct
     1441 ggcagtaggc gcagtgaagc ggacagccac tgaaaaagat catccccgat ccgacctcgc
     1501 cgctgatggg cggctcctct ccccggtgca atcccgccca ggcgatccga accgtcgagg
     1561 attcattgca gactcccctc tccccttcaa gacggttgac gaggcagcga tgaggacaga
     1621 gagcgcacgc ttcataagga ctcatacggg tatcatagcg aaaacgggaa agaagagaca
     1681 actcttatta ttctgcttct ttcattcctt tatcggtata ttatctattc cgtggcttgc
     1741 agtcttccct ttgcttgacc agaatcggaa aaatgctcac tcttaggata tgccttggcc
     1801 gtttttacca tcgagcaaac cgaagcctca tgaagaacat cgttttatcc tttccatcga
     1861 tgggggcgga atgcgcggaa tcgttccggc tgcgattctc gggcggcttt cccatcaatt
     1921 ggaggttctg ggatcaaaca ttcccctcta tgcctacttt gatctgattg ccgggacttc
     1981 gaccggcggc cttttggctc tgggcttgag cgttcccgct tccaaatcca atctaccggg
     2041 ggacggtaaa gacgcaattc ctgtctatga agatattaag ggaaaatggc tcttcggcgg
     2101 gaaacccaag caacgctatg tgggaacgat tcccccgtcg atcgatctca gcgccttgga
     2161 ggagttgtat tttgaacggg gctccaccat tttcccgaaa agccaaacaa ggcgcatcgg
     2221 gcagatgttt atcgacaagt atgatgagca gccgctggag cgcttcctct ttgaaatgtt
     2281 cggcaatacc ccgctggatg aagcgatggt tccaaccctg atcgtcgcct atgacagcga
     2341 tgagggacag ccgttttatt tgaccagcta tgagagcggc tccttcctgt attgggaagc
     2401 cggacgggcg accagcgccg cgccgacctt tttccggcct gcggtcctgt atgaccgcca
     2461 aaagcagcga acggtccgct tgatagacgg cggcgtcatc gcaaacaatc catcgctttt
     2521 tgcctatcag gaggccaaaa aattgtatcc cgacgccacc cgtttccata tcctctccct
     2581 ttcgaccgca gggataaact acacctttaa aaccgatgga gccgcaggta ccggggtgat
     2641 tggctggatc gacccgacca aaggcgcgcc gattcaaaag atctatgcca acgcccaact
     2701 gcaggtcgtt gattcgatcg ctgaagcgat ccccgatctc acctataccc gggtacacgg
     2761 agaagcgcgg cgtgattata aaatggatga aaccgatccg agaatgctaa aggacctccg
     2821 ctttgatgca gaggagatct atgcaaagaa tgaagaaaaa atcctcacct tcctgactgc
     2881 cctggtgaag cgaaccacct tcgatcacgt tcgcttggag gagagggcaa atgagtagtc
     2941 atcccaaaca gctcgccctt gagcaaaccc tccttgagct cagtcacgcg gtggacgatt
     3001 atctggaaga tcgatatcac gattacttta ttcgtcaccc caaccggccg aaacggggca
     3061 gtaccgcaag cgcttcccac gacgggctgt tttcaatcgg aacgcagttc accgccggat
     3121 atggcagcga cctggggcgc ggctacatcg tcgtcatcga gatacgaacc ctccagaacg
     3181 tcccgtcggt catccgccga aagatcgaag aggatggcat cgaatacctc agaacccagc
     3241 tccccgtttt tttccccgat cggaaaatcg aggtgaaaaa ggatgggaat ctctataagc
     3301 tggttgggga tttctcgttg ggatctgtcg gtcattgata aggaaggatt cctaactaac
     3361 tgcaaaagct cctcttcttt cgaacaaaaa ttgatttcta cttacactag gtaatgctcc
     3421 attattattg atacataaag gagttgtttt cttatgaaat attgttagga ttgtttactt
     3481 tcaattcgat tcagcgccaa ttccccggtg atcgaggtaa ccgacacccc atggcgatag
     3541 ggatatccaa ggttttgggt cttcttgatc gcaagcgcgg taagctgtgc ggctttcgtt
     3601 acgcttaatc ggaaggggtc cttatcaaga agataggcaa gaaggatcga ggcgaaaaga
     3661 tcgccgcacc ccgggtagga agtgtcgatc tgatcgtagt acacaaggta tgagccatct
     3721 ttttcggtgc aggcgacggc catcttccct tctacgggga ctgaagtgat cgctacattc
     3781 gccttgcttt ggtatgccag ctctttggcc cactgaagga tcgtctgctc atcaaaacgt
     3841 tcccggtagg gataacccag aagcaacgcc gcttcagtga cattgggaca gatgcaatcc
     3901 gcttcggtga cgagcttttt catggcatcg atgtgctctt tctccatcgg tccgtacagc
     3961 tctccatcat cgcccatcac cggatcgacg aggaccagcg gcgggttctt tttggcccga
     4021 tggaagtgga tgaactcggt gatgaggctc acctgctcgg cggaaccgag aaagccggcg
     4081 taaatcgaat caaacgcaag atcgagtttt tgccattcgg cgatgatcga gcgcatctct
     4141 tttgtaagcg gttggaagaa gtaggaatca aagccgtcgc tttgggaaga aagcagagcg
     4201 gtgggcaatg gagacacctc taccccgagc gcttcgaggg cgggaagaac gaccgtcagg
     4261 gagctttttg catatgaggc cagatcatgg attgcgacgc atgttttttg catggttcta
     4321 ccctatccta catcctcttt actgacaata tatgctacac tcaggagtat gattatacac
     4381 gatactctca atgatcaagc aattgcagat tggcggacaa accccctggc attcccgctt
     4441 ccttccaagg aatccttgag cgatgcatat acgagcatga tcatctcctc ctccggttgg
     4501 cggaaggtgt tcgctcccgg cggagatgaa gaagactccg gccggacggt caatcctccg
     4561 gatcgaattc tggcggcgtt ggcggcgtac gccctctacc tgcacgtagg caagaagaag
     4621 ccgacgatcc tcgtcggcct tgacgcccga ccgaccggag cccatcttgg ctccatcgcg
     4681 gtttataccc tcctttccct tggggcaaag gttcgctacc tcttcatcac cgcagcgccc
     4741 cagatcatgg ccgataccaa tctccacccc aaagatgccg atgcatttct ctacatctca
     4801 gccagccaca accctatcgg ccacaatggg ttcaagttcg gaaaagaagg gggtgtatat
     4861 ggctccaagg atgccgaacg tcttgccgag cgcttttgtg agctggttgg cgacggtctg
     4921 acccacttca cccttcttca gacatacgca gagaaccttg atcctcaaat ctataaacga
     4981 acccttgagc gggtcggcga agaacgggaa aaaagcgcag agcgctatcg cacccttgct
     5041 ttgaggatcg cgaccaaaag cgatgatgag caggctcata cccggttcat cgaaacgatg
     5101 agaatcgagc atcagcagaa gccgcttgga atcctcggcg aactgaacgg ctcggcccgc
     5161 agctgctcaa tcgatcgccc cctgctttcg gagcttggga tcaccaccga attcatgcac
     5221 gacacccccg gggatgtggt tcacccgatc gttcccgaag ggaagaacct caccctctgc
     5281 atggaatcgc ttacagccct tcatcagaag aaccccgcgt ttgtcctcgg gtatgtcccc
     5341 gacaatgacg gagaccgggg aaatatcgtc tacatcaacg acaggaaaac cgcgacgatt
     5401 ctggaggctc aatccctctt tgcgttgatt gttctctccg agctcgccca aagccgactt
     5461 tccgatcccg atgcccccct ggcggtcgtc gtgaacggac cgacctccaa tcgcgtcgat
     5521 gagatcgccc gctcgttcgg tgcgaaggta tttcgcgccg aagtgggaga agcgaatgtc
     5581 gtggagcttt ccaatctcaa gcgaaaagag ggatacgtcg ttccgatcct cggtgaaggc
     5641 tcgaacggag ggaacatctc ccacccggcg aaggtccgcg acccgttgaa caccctgctc
     5701 tctttgacca aactcttaaa acaagaggaa ctaagcaggt tgtggttcac cctctcggga
     5761 caaccgcaac ccgcctcgtt gacgctcgat caattgatct gttcccttcc ttcctatatc
     5821 acaaccggag catactcctc tttggcgcag ctgagcgtga acattgaaca cggccggctg
     5881 aagacccggt acgaggagtt cttcgaagag atcttcaacc gtgatcacat cctgttcaac
     5941 agctttggaa tcacctccta caagatcatt caaacggaag gaaccgaaga gcggatcgga
     6001 agcggcccgg cataccgcac tcctccgtac aacggcggct tcaaagtcgg gttgtacaac
     6061 gatgagaacg tgatgaccga ttttctgtgg atgcggggtt caaagacgga gccgatcttc
     6121 cgcctgctcg tcgactgcaa gggtgacaac acgaatcggc acgattatct tttggagctt
     6181 caccgcctcg tcatcacccg agctctccaa tcgtagttac agccccaacg cagccacggc
     6241 cgagccgatc gtcggtgaat catcgattcg gacgaagcgg tagtagcgct tgtatttccg
     6301 ctgcaggtag tggtggaggt agaagttcgt gtattcaaga aggtattcgg ttttatagag
     6361 ggtcgttccg tcggcgttga tgcacaccgg tttccggggg tcggtccccg atccgctctt
     6421 gaggatcgtt gatgtgagat tcgccgcggt cagtttcgcc gcccgttcga ttacggcgtt
     6481 gatgatcagc caaagaacgc gggcatcgcc ttcattgccc tcgacacact ggacaagttc
     6541 atagtcgcga ttaaacggca tctcgaggta gttgctcatt acggtggttg aaagcgtctt
     6601 gatccgcttt gcatagcgct ctttaaatgt ctcggaaaag agcccggcat cggccgcttc
     6661 ggtgatgatc gaaccgcaga tcggccccag ataggcgccg ctgatcatct tctcgaggtg
     6721 gtattggttg ggctccttgg tggaatcgaa aaacttccgg tcgaaggggc cgggacagta
     6781 ggcgaagttt cccgattcga tgttgatgat ttgggctttg gaagggtcgg cggaaagctt
     6841 ggtaatcttt ttgtttgact caacgtaggc ggtgttcgtc cccgtgccca gaatgaagcc
     6901 gatatagccg ctgtagggaa catcactcgg agttccctga ccggcgagca gcgtcgccac
     6961 cgtatcgttc aatacggcga cttttttctt ccgcacatcg tgtccgcggc ttgccagctc
     7021 ggcaagcaga ctcctgccaa gcggcatacc gatcacttcg ggagctttga tctctttgga
     7081 gaacactagg ggaattccat cccggtcgga ggtgatttca gcggcgtacg agaagcagaa
     7141 ccctattcga tcactccgat cgatgagtcg ttcgacctcg tcggcgatga ttgaaaagaa
     7201 ctccttcgca gtcacttcgc ttttcacccc cggcatcgag gtcttcttga actcggagat
     7261 tcgggggttt ttctcccggt caaaccggac gagacaagtc cgaaggttgg tccctccggc
     7321 atcgatcacg atgaccggct gatcggccag aatggtgtca cttgtcgtgg tataggtcgg
     7381 aatcatcttc agtgacgaat caggagcacc ggccagacct ttttccatct ccgacaagaa
     7441 cgatgtgatc aactgatcca tgtcaacctg atcggcatcg atcttggagc gattgagaaa
     7501 ctcttcggtc tttttgatga gctcgtgcat tgtttattct ccgtttactt tcagttctct
     7561 tggctcgatt acaaaatacc cttcctgatc ggtaaaaagc aagaacgagc cgctgattga
     7621 tacatcggtg gacagccggg tgccggcggc ttttacatca acgatcgctt cgatcggatt
     7681 gatcgctcct ccggtaacca cgctggtcat cagactcgcc aaggtaactc cttctcccca
     7741 aacggtgctc acccgtccgg tgagcgaaac ctgtccctct tcccattcat gggtatcgag
     7801 gaagagaagc gccacatcgt tcaacggacc tttggcgctt tgaagcaatg agagcagaaa
     7861 gttgctttgc ggcttgttca acgattgata cgtcttccga tatatgccga gatccgcatc
     7921 cttcagctcg agcgctttag gcagcgtggc ccgcgcctct ttttcaaaga ttgatccggg
     7981 caggttcaac gcgggggtat tgagatacgc ggcgacgttg gcgatcgtgg cgtcgctgat
     8041 cgcggtgaca gcgatcagga tgtcccgttc actcggcttt gctgccgtga gcggggcaat
     8101 gatgaggaga agaatgagaa ctaatccaat ggtttgacga ttcatcttgt acgtatcata
     8161 aagggaagaa tcgattaaga caatgaagaa gaggtgtgat acactgaagc aatgattaaa
     8221 ccgtcaattc tttggtatga ccttgaaaca ttcggccttg acagccgagg ggaccgaatc
     8281 gcccaatttg ccgcgatccg aaccgatttg gatcttcggc agatcgaaga gccgatcatc
     8341 ctctactgca agctcagcga tgattacctc cccgatcctc tttcctgcct ggtgaccggc
     8401 atcacccccc aggaggtgaa tgcgaagggg ctcgtcgaag cggagttcat tcagctgatc
     8461 aacaccgagt tcagcaggcc cgacaccacg gtcgccggtt tcaattcgat ccgctttgac
     8521 gatgagttca tccgcaatgc cctctatcga aactttttcg atccctatcg acgggagtat
     8581 gaacacaaca actcccggtg ggacatcatc gacctggtcc gtgcgaccca cgatctgaga
     8641 ccggaaggaa tccggtggcc tgagcgtaat gaagtgaacc gccccgtgtt tcgactcacc
     8701 tccttgaccg aagcgaacgg aatcgagcat ctcggggcgc acgatgccct ggctgacgtc
     8761 cgggctacga tcgcaatcgc tcgtctcatc aaggaaaaac agccaaggct ctatcagtac
     8821 gcctatgacc tgcgctcaaa gaaccgagtg aaaagactgc tgaaagcccc ctttggcggg
     8881 gagcacacct ccctgattct gcacaccaac cccctcttct cctcggaata tggcaacacc
     8941 cgcctcattc tgccgatcac ctacctcaaa acgaacgcca atgcggtcgt gtgcttcgat
     9001 ctgagctgcg aagtggaacc gctcctctca gcgacggaag agacgctgat gaatacgccg
     9061 ggagtcttta cgatttcgat caacaaatgc ccgttcgtct caccggtgaa cgtgcttacc
     9121 gaggagctgg aacggaagct tgggatcgat tccgccctcg ctgcgacgaa agccgcctat
     9181 atcaggacgc acccgaacgt gatagccgtg atccaccggg tgaaagacac cttcgagcgg
     9241 gtcgacgacg tcgattacca gctctacgac gcgttcttca ccaacaggga ccagcaggtc
     9301 ttcgagaccc ttcgccaagc gaagccgagg gaaaaaatgg gctttctcaa tcaggttcag
     9361 gatccccggt tcggaactct cctcttccgc catgtcgggc ggaattggcc ggaggcgttc
     9421 accgaagagg aacggaaacg ttggatcagc cacgctgcca atcgcaccct caatccaccg
     9481 ggcgacggca aaatgacgtg gaagttcttc tatcggagca tcgaagagaa actcgagtcg
     9541 gtcgacacca cccccaggga gaaacagctc cttgccgagt tgaaagagta cggcaccgct
     9601 cttgaggcaa agattttcgg gtaatacccc agctatgcaa agcaaacctc cttttgtata
     9661 cttcctctat gcttgagaag ttagccgaat accaaagcca attggaacag atcgatctcc
     9721 aactcgccga cggtgcgacg atgagagaca tgaacctcta caagaaactc acccaggaac
     9781 gcagccacct tcagccgatc gtcgatgagc tctcccgcct tcaggagatc gccgatcaga
     9841 ttgccgacgc gaaagaactg ctgaaagagg agacggacac cgagatgatc gccctgaccc
     9901 gcgaggagct tgagaatctt gaagctgcat tggagaaaac aacctccaaa gtaaaactgc
     9961 ttctgattcc ccccgatccg ctggcgggaa aagacatcat catggagatt cgagcgggca
    10021 ccggcggaga agaagcggcc ctctttgccg ccgatctgtt tcggatgtac tcccattacg
    10081 ccgacagccg ctcctggaag atggacatcc tctcttccaa cgagacggga atcggcggat
    10141 acaaggaact gatcgtttcg atcagcggca aggatgtcta cgcatcgctt cagtatgaaa
    10201 gcggcgtcca tcgggttcag cgggttccgg aaacggaaag cggcggaaga atccatacct
    10261 cggcggttac cgtggcggtg ctggctgaag cggaagaaac cgatatcgag attcgcacgg
    10321 aagacctcaa ggtcgagacg atgcgctcca gcggccccgg cggtcagagc gtcaacacca
    10381 cggacagcgc ggttcggatg acccacctgc ccaccggcct cgtggtcgtc tgccaggatg
    10441 agaaaagcca gatcaaaaac cgggcgaaag cgcttcgagt gctccgttcc cggctcttcg
    10501 agatggaaga ggagaagaag gcgaaagagc gggcggagga ccgcaagagc caagtgggct
    10561 cgggggatcg aagcgagcgg atccgcacct acaactttcc ccagaaccgc ctcaccgatc
    10621 accggatcaa ccttaccctg tacaagcttg atttgattat ggccggcaat ctggatgaag
    10681 tgatcgagcc gctgaagatc gccagcggag aggctgcgct tgccgaggca taggatgacc
    10741 ctcaacgcgt ggattgctga aacgaaaaac ctcttcattg aacagggagt cagcgacacc
    10801 cccggtcttg atgcccgtct ccttcttgga cacgcaaccg gatatgatca gggaagacag
    10861 atcgtcaatg ccggggagga gctcgctatc catcagctcg atctccttga aaacctgaga
    10921 tctcaacggc ttgcaggacg cccgatcgcc tatatcatcg gttccaaaga gttctacggg
    10981 cgctcgttct acgtggatga acgagtgctc attccccgtc cggatacgga aattctcgtt
    11041 gagaccgtcc tcaaggcgat tgaggatggc gtcgtctcct ctgaagattc gatcatcgac
    11101 gtgggcaccg gcagcggagc ggtgggcatc accctcgccg ccgaactcga ttgtatggta
    11161 ctcttaagcg atatcagctg cgatgccttg gatgtcgccc ggatcaacag ccggagaatc
    11221 ttgggaagag agcttgagac gattgcaagc gacctgcttc ctctcgggcg aaaatatggt
    11281 atcatcgtga gcaacccccc atacctcacc gatgagtggg ttgcagcggt tgatcaaaac
    11341 gtccgatctg aaccggatct cgccctgcga gggttcggag aagacggact cgatatcatc
    11401 agggcgctgg tcgctgagag ctcgaaccat ggttcgctct ttatcgaatg cgactatcgc
    11461 cagacgaaac aggtagccgg gctctttgaa gagcatggct ttacgaatat atcgattcaa
    11521 cgcgaccttc aggggttgga acgtgttgtt tggggagtac gaacgtgaat gcccagttga
    11581 ttgagcggtt tattcaaaaa gcgttcaaat atccgcagga agatcaggag aagatcctgg
    11641 ctgcggcgac gttcgccgac tccaagcatg acggccagaa acgcgccagc ggagagccgt
    11701 atatcatcca ccctctggcg gtcggcgaaa tcctcatcca attgaagatg gacgccgaca
    11761 cgatctctgc cggagtcctc cacgataccc ttgaagacac cgataccacc tacgaggaac
    11821 tgtccagact cttcggcaaa gcggtagccg acatggtgga gggcgaaacg aagatcgcaa
    11881 gcatcaagac gatgagcaaa tcggtccaag aggcggaaac gatccgaaaa atgttcttcg
    11941 cgatgagcga agacattcgc gtcatcatca tcaagcttgc cgacaagctg cacaacatgc
    12001 ggacgcttca ccatctgaag aatgagcggg cacgggaaat cgccaatgat acgctggaca
    12061 tctacgcgcc cttggcggac cgcctcggca tctcctggat gaaggacgaa cttgaggacc
    12121 tcagtctgaa aaccctcaag cccgacacct acaaccacat tcaggaatat cttctgtcaa
    12181 agaaaagcga gcagaccgcc tacctgactc gagtggagaa atcgatctat cgggcgtgcg
    12241 gggatgcgaa catcgccgat gtagtggtca ccagccgcgc caaacatgcc tactccgtct
    12301 acatgaagat gaagaagcgg aaaaaggaga ttgatgagat cttcgacatc ctcggagtgc
    12361 ggattctctg cacctcggta atcgactgct acacgatcct gggggtcgtc caccgcctct
    12421 ggcccccgat cgaaggacgg ttcaaggact atatcgccat gccgaaggcg aacaactacc
    12481 aaagcctcca caccaccgtg atggcgctcg acggcaaact tcttgaaatc cagatcagga
    12541 cccacgaaat gcatttcacc gccgaatacg gtgtcgccgc ccactggtcc tacaaagcgg
    12601 aaacgggcag tgaaagcggc aattgggcac agatggacga cgagcagttc accaagatcg
    12661 tcagcaagct caaagcatgg tccagcgaaa ttgaaaacag cgaatcctac atggatgaca
    12721 tcaaaggtga tctcttaagg gatacgatct acgtcttcac ccccaaagga aacatcgtcg
    12781 agctgccgaa gaacgccacc gcgttggatt tcgcctatca gatccacacc gaggtcggaa
    12841 accggaccac cggagcgaaa gcggacggat cgatcatccc cctgaacgct ccgctgaaaa
    12901 acacccaggt catcgagatc ctcaccagcc cgaacgcccg accgcatttg aactggctgc
    12961 gctacgccca gacgaacagc gcccggaaga aaattcgggc gtggctgaac aagtacgatg
    13021 agaacatcct catcgacaaa cagatcatcg ccaagcggaa agagatcgaa caggcccaag
    13081 cgaagatcga aaaacggccc cctgaacaac aggtcgaagg ggacgatgat gagatcatcc
    13141 gccaacacgt cgatccttca cggatgaaat tccgggtcgg ggacgagaag aacatgatga
    13201 tccacatcgc ccagtgctgc aacccggtca gaggcgatga gatcatcggc tacgtcagcc
    13261 ggggccgggg aatcatcgtt caccgcaccg attgccccaa cctgaaaaac atggccgagg
    13321 tgaaggaccg gtcgatcgac gtcgaatggg aaacggaatt ccccaagctg accaagcggt
    13381 tcgccgtgac gagcaaacgc acctacgacc tcttcggcga gatcgaaggc gcgctcagga
    13441 agtataaggg ccacctgatc gaaggccgcc ttcaggacga tgaggacggc aagctgatgg
    13501 gcaccttcac gatggaggtt cagcaggagg atgacttcaa gaagatcatc aagaccctga
    13561 aaacgattcc ttcgatcaac acgatcagcg agattcgcta gattactcct gaatcacccg
    13621 aatgatctca ggcagcagct gctttttacg ggagaggact tgcggcagga gatacatccc
    13681 ttcaccggcc ttttcgtagg cgagcttctg ctcggcgatc ggcatccggg taaggagcag
    13741 cacggagttc tccttgacga cgtcggtgat caggaagagc gcccagtcga ggctgtaggc
    13801 ctttttcacc atctccagct cctcaagata ccgagcgcga taatcctcta cgtcatcaag
    13861 ggtggttacc tcgctttgac cgatgccgaa gttgactccg ctttcccgat agcgcttaaa
    13921 gtcagcctca atcatcattc tggcatcact tttagccaga gaagccccgc cggagaacat
    13981 cgtttccccg aatttgtaca tgtcatccac tccggcgatc gagagaagat cctgcactgc
    14041 ggtgtagtct tcgaatgtcg tggtggggct tttgagcatg atcgtatcgc tgacgattcc
    14101 cgaaaggagg actttggcga tatcactcgt cacttcaacg ttgtggcgca tgaacagctt
    14161 gtagacgatc gtgcaggtcg aaccgagcgg ctcgcagcag atgaaaatcg ggttgcgggt
    14221 cttcgccgcc ccgaggcggt gatgatcgac gatctcgacg acctcggcct cctccacccc
    14281 ggtgatcgcc tgatcgtttt cattgtggtc gaccatgatc agtttggttg ccggcttttc
    14341 caaaaaggaa cgccgcgaga cgtagccgcg gtattccttg ccgctgaaga ccggcagctc
    14401 cctgtaatcg ttgttggcga gaaatgcccg cgcttcgtca aagagcatat ccgcctgaag
    14461 cgagggaagt ttttttacga gcagttcact gaccggaacg cacaggcgga ggagtcgaag
    14521 cgtttcggcg gtgtccgttt cgctgacgaa cacgctcccc tcataggaat cccaatctat
    14581 cttggagatt gttttcccgc ggattccggt gaggatgatc gcaggaacgt tcaattcgat
    14641 cgctttgttg atgtgccgct cccgatcgcc gacgacgaga atcggcatcg gtcccttgaa
    14701 gcccttcatc accttttcat aggtcgcgaa cttattcgcc ccgaccgcca aaggcgcaag
    14761 gatcgattcc tcattccctt ttttgatgaa gtatccggga atgacgactt caaagttttt
    14821 gacgagaaag ttgtagaccg gccggcttcc cagattctcc ttgaggataa aatcactgat
    14881 ctcatcgatc gtcaccaacc cgacaaaacg ggttccttcc atgatcggga agacggagtg
    14941 gcgtgcagta ttcttataga gggtgatcag gcggtagacg ggatcggtgg gacacacttc
    15001 gacatccgat tcaatgatga ctttggagac tcgggttcgc acatctcgtt taaacggcgg
    15061 aggtatcaac ccgaatcgat tgaattgagc ccgggttgcc tcgttgagat ttccacaacg
    15121 aatcggaata taggtattgc tcggatcgac cttgtttttc agcgcggcat atgcataggc
    15181 gctgcagatc gaatccatat cgggattctt gtgtccgctg acgaaaatct ttgccactgt
    15241 ccactcctgt tgtccctatc gtagcgtaat ttcaaggggc gcacaactga gacgagctgg
    15301 aaaaccccgg tggaaaagct tgttgtttca ccggccaaac cctcctcttc tcattggaaa
    15361 tacttgcttg ttggggtaga atcgaagcgt ggaggaatcg taatgcctaa agtagttatt
    15421 gctaatgatc atggtgcagt tgagttggca gttactctca cagagtatct gaagaagatg
    15481 ggttacgagg tgaaccacct cggtaccaca actgaggatt cggtcgatta cccggatatg
    15541 gcgctcgcag ccacaacgga gttcaaaaaa ggcggatacg agtttggaat tcttctttgc
    15601 ggaacgggaa tcggggtatc gatcagcgca aacaaagtcg atggaatccg ctgcgccctc
    15661 gtccacaaca cctacaccgc ccaaatggcc aaacggcata acgatgcgaa cttcatcgca
    15721 ttcggcgggc gggtcgtcta tcgcgaagag gtcgaagaca ttctcgacgc gttcatcgac
    15781 gccacctttg aaggtgagcg ccaccaacga cggatcgaca agatcatggc gcttgaaaac
    15841 cggaattaaa accgtcttga aggaaagatg aaggggtgcg gaaggagctc gtcaaagcga
    15901 cggaccagat gcaccccgtc tcttccttca gccgcatcga catccagaag atgaacagcg
    15961 gtatcactgc ggctgaattc agcgagaacc tgtaaacact gggcgcacgg cggtgccgga
    16021 ggagaatcgg agctgaccac caccagacac tcaattccga cgacaccttc atcggcaatc
    16081 gccctcaaca gggcgctgcg ctcggcgcag atcgtcgccc cataggagga gttctccacg
    16141 ttggcccccg gatacacgcc cttggttcga tggcttacga tcgcactgcc cacctgatag
    16201 ttcgagtacg gcgcatacgc attgaggcgc gccttcaacg cggcggcgat cgcctccctg
    16261 agaactccct catccatctc tttgacggga tcggcgatga gattggctcc aaacggggtg
    16321 ccgccgtgaa cgccgacata ccctcgaagc agctcattga actcattcga ggtggaaaaa
    16381 tcatcaaact catcaagggt gcccatcacc gcatccgccc cgctctcaaa gagctcttcc
    16441 cgcccgaagg tggtggtcaa gccaagaacg gtgcagccgg cggcttttcc ggcgaccgtt
    16501 ccattgaggg catcttcgac gaccagacac ttctttgagt cgatgcccaa gaggagcgag
    16561 gcgatttgat aaatatctgc atgaggcttg ttgcgtttca ccatgtcccc ggtgacgacc
    16621 ttgtcaaaaa tcgattcctc gaccccgacc gcttccaagt tgagcagcat cttcatccgg
    16681 actgcgctgg tgacaagggc gatcttcatg cccgcccgtt tggcgtttgt gatgaagcgg
    16741 atgactcccc ccagcggtcc gctttctttt gcaatccggg cgtagtggct gaagagaaca
    16801 agcttttcca cctcgaagtc cagatcaagt ccatactttt cccccacccc caagagatac
    16861 cgcttatcac cggtgccgat gaaaggagta aaatcttcct tcttgacggt cacaccttta
    16921 tcggcaaaga gccgcatcgc cgctttgaga ataatcggct cggagtcgat cagcactccg
    16981 tccatgtcaa acaagatcgc ttcaatcatc agtgcatcac cgtttttcct cgtcccaccg
    17041 agtgttgggg aatgaattcg ccgccgcccg ccaacccgag atacaccccg tcgcccatga
    17101 tgagcctgcc gccaaggtag gtcatcagcg ctttcccgac gacggtcatg cctgcatatg
    17161 cgctgtatcc gcttgccgag tgaatggagg catcaccgat cttccactga tggtcgggat
    17221 cgaagaggac gaagtccgcg tcgcttccga ttcgcaaagc tccttttcgc ggatagaggc
    17281 cgaacgcttt ggccggagcg gtcgagagaa gattcacgac ctgctgaagg ccgatccgtc
    17341 cgctcttcgc ggcaaagttg tagaccaggg agagcagttc ttcacttccg gggataccgg
    17401 gaaggatcgt ccggctgtcc ttggaggaaa gcttctgttc tttcgtgaag gcgcagtggt
    17461 cggtcgcgac gacttggatc tctccgttca ggagcgcttc ctgcaacgcc aaccgatctt
    17521 cctcatcccg aagcggaggg gtcatcaccc acagcgcccc gtcggcgccc gcaagcttct
    17581 cgccggtcag gaacaggtag tgcggcgtcg tttcaacgat cacccgcagc ccttgccttc
    17641 gaaggtcgcg aaccgcctcc agccctgcct tgctggaaag gtggacgatg tagatcggga
    17701 tgccggcttc aagggcaatc ttccccatcg tccgaattcc gaccgcttcc gcttccgaag
    17761 gacggagaat cggatgggaa gcgggagcat aatcaccttt gtatgccttt tcagcgcgtt
    17821 ggatgacctc gtcatcctcc gcgtggatcg taacaagaag atcaagctct ttacagatcg
    17881 caaagatctt tttcaattcc tcttcttttt cgatcatgta ccccacgttc ttgtaggtgg
    17941 tgaagagctt gatgaccttg actccggctt ctttgagctc aagcagctcc tctttcaggc
    18001 ttgggcggta ggaataaatc ccttgatgaa gggaaaaatc gatcgccatc tcttttccca
    18061 tcgccgcaat ccgttctttg gcattgacca agagcgatcg cttcttgtca tcgtcggcaa
    18121 agtccaccac ggtggtaacc ccgccatagg cggcggcccg cgagccctcg gcgaaggagt
    18181 cggcggtaac cgtgttgcga cttaccaaat gatagtgggt atgcgcatcg atgataccgg
    18241 gaagaatcgc aagcccgtcg gcccggacga tctccaccgg accgtcaatc tcaccgacct
    18301 cgatcttcgg agcgatccga acgatgattc catcctcgat caggatatcc gcatgccggg
    18361 tccactcggt atccaccagc aaaccttggg taaccagaag tcttttcata
//
LOCUS       DAOSLI010000038        17834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3375_length_17834_cov_23.680916, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000038 DAOSLI010000000
VERSION     DAOSLI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17834
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3375_length_17834_cov_23.680916"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLF67_06460"
     CDS             <1..555
                     /locus_tag="PLF67_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOE89690.1"
                     /translation="SELDTLNRGGTNLPSHCDHLRTKGIDVTTGSLGQGLSVGCGVAY
                     SLLHDGKQQYVYVVIGDGESQEGQIWEAAMFAAHYKLKNMIVFTDYNKLQLDGKIDDV
                     MSLGNLAEKWRSFGWDTRQVDGHDLNAIHEAVINAQNQQTRPAMIILDTIKGKGAAFC
                     EGLESNHNMSFSMETAKQAIAELG"
     gene            566..1507
                     /locus_tag="PLF67_06465"
     CDS             566..1507
                     /locus_tag="PLF67_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOE89691.1"
                     /translation="MNQKEMRTVYAETLIELAHEDSRIMVLEADLMRAHGTQIFKDVF
                     PRRTVGVGVAEANLVGVSAGLSVAGKIPFAATFATFASRRAFDQFFISANYAGLNVKL
                     VGTDPGVSAAFNGGTHMPFEDIGLMRMIPDLVIVEPADPTSLKEIIRLCAAHQGNTYL
                     RLHRKALPQIYQTGERFELGKGKLLHDGYDITLVASGGLLVNEALKAGELLKNQGISA
                     AVIDMHTIKPLDEQLILEYAEKTGAIVTAENHQVAGGLGAAVSLFLSTRHPIPMEMVG
                     IHDRFGQVGTQDWLMQHYKLTADEIVRKAFLALTRKH"
     gene            complement(1504..>2429)
                     /gene="larA"
                     /locus_tag="PLF67_06470"
     CDS             complement(1504..>2429)
                     /gene="larA"
                     /locus_tag="PLF67_06470"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOE89692.1"
                     /translation="ARRLNNRIVSHVATDRSRLTYLGTTSRGTPVALNSLLFEVDRVV
                     VTGSVEPHYFAGYTGGRKAIMPGIAGYETIEANHRLALGSAAHTLALEGNPVHEDFLE
                     VVEFVKQPIFSLMSVLDKDHRPIHLAGGTLTESFLECIPVAEETFGTPIASPMDIVIT
                     VANHPMDINLYQAQKALDNGALAVRDGGSLLLVASCREGVGGAAFADLLASSESPDQA
                     LAAIHEHYKLGYHKAAKIALEAKRINLYAYTDLDDERVSSLFLKPVKDLQRWFDRVVD
                     EFRQAHRRDPRVLVLRDGGMCVPILSSSGKH"
     assembly_gap    2430..2529
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2530..2936)
                     /locus_tag="PLF67_06475"
     CDS             complement(<2530..2936)
                     /locus_tag="PLF67_06475"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOE89693.1"
                     /translation="MDSIVLPYRSGEHLVLKAPKALGVSVRDPKEGGLRARKHLFKEA
                     LGTGFADFLRSASRVLIIINDSTRPTPTKEMMSALLPYVEEAGIQDDQLTILIATGAH
                     RPALESEFEQLLGRGLARRLNNRIVSHVATDRSR"
     gene            complement(2940..4166)
                     /gene="pgk"
                     /locus_tag="PLF67_06480"
     CDS             complement(2940..4166)
                     /gene="pgk"
                     /locus_tag="PLF67_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOE89694.1"
                     /translation="MKSIEGVDVRSKRVLFRPDINSPIDQKTKRIVNTNRLEKAAVTL
                     KLLLEGGAKVAIIAHQGDTMDYRNIIPMAEHAEILQNLTGFPVSYIDDVCGLAAQEAI
                     ESLSESEAILLGNVRYLSEEITAFEKEVKLTPTEMQSTYLIRSLKDHFDLYVNDAFSA
                     AHRSTPSMVAFQEFLPTYAGRQLMDEYSALSRISEGTAHPRVFLLGGGKISDAFGMMD
                     HVLSTGSADAVLTGGLTGHVIALASGVKLGSATEAFLAEKDFIRFIAPAKKLLEEFPK
                     KVFFPLDFAFEKDGERAECTHEEVEASDDRFYLDIGAKTIALYTEHIARANTLFVNGP
                     AGVYEDERWENGTRELLRAVSNSTAYSVIGGGDTITAAARYTGLDGYSYVCTAGGAMI
                     QFLSGKRLPLLEAMKA"
     gene            complement(4166..5200)
                     /locus_tag="PLF67_06485"
     CDS             complement(4166..5200)
                     /locus_tag="PLF67_06485"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOE89695.1"
                     /translation="MSKRVKVGVVGYGVIGQRLADGVALQGDMELVGVADVGPTLSIR
                     ALRERGMPFDFYLANPANRGVLEELGVPVTGSLEELVQKCDIILDATSAGVGLKNKEL
                     YEKHRKKAVFQGGEKNAIADVFFHGYANYEQGVGQSYLKLTSCNTTGLIRAVDCFDRA
                     VGVEKVAITIIRRVADPGDYHRGLTNALQMDKAPSHQAVDLMTIMPHIEATGILVHTP
                     VTHGHIITVVLTPKKDLSVEEAVAHLKAHPRIRVVSIDEGFLGNASLFRYARDLGNPR
                     GDMYEIAVWKDSVVKSGKDIMFAINIPQEAVVIPESIDAIRSALKLQTTREEGTSKTN
                     EYLGIGRWNR"
     gene            complement(5214..6107)
                     /gene="iolE"
                     /locus_tag="PLF67_06490"
     CDS             complement(5214..6107)
                     /gene="iolE"
                     /locus_tag="PLF67_06490"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HOE89696.1"
                     /translation="MSNTRIKLAIAPIGWTNDDLPQLGGEIPFEQCVSEMALAGFEGS
                     EVGNKYPKDPAVLRPALELRGLRICNAWFSTYLTTRPYEEVERAFVEHCTFLQAMGAK
                     IVGVAEQGNSIQGDPDIPVFDGKPVNSERQWELLAAGLDKLGKKAREFGLRLTYHHHM
                     GTGVQTNGEIDRLMEETDADLVGLLYDTGHLVFSGEDPMVPLRKWFDRIWHVHLKDVR
                     AAKRQQAIKEKWSFLKGVLEGVFTVPGDGMIDFRPIVEHLLANGYEGWWVVEAEQDPK
                     IANPLEYAMKARKYIRDIAKI"
     gene            complement(6116..>7374)
                     /locus_tag="PLF67_06495"
     CDS             complement(6116..>7374)
                     /locus_tag="PLF67_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOE89697.1"
                     /translation="KRVHYIERRIPTVGQLDRLAKLIKTGKKPLVIAGGGIRYSAAGA
                     ELASFCETFHIPFGETQAGKGTIVWDHPYNLSGIGTTGSLAANRLAKEADLIIGVGTR
                     FGDFTTCSKWLFRNPRASFVSINVASFDGYKLNAEPIIADAKLTLEAIVRHPELRSYR
                     SEWGNRIREEKAVWDREVEQLYHEELEGTLSQARVLGELNDRLLPPDAIVLSASGSIP
                     SDMQRVWRTRVPGTYHMEYAFSTMGYEIAAALGAKIAHPEREVVALVGDGAYTMLHTE
                     LLTSIQEGKKIIVVVLDNAGFHCIDNLQTSQGIVRYGNEWRKREEQSGRLSGQSLVVD
                     YALNAQSWGAVGLRATTIEGLAEAVQQALKEEVSTVIHCLVAPKSMTGNYEGWWRVGT
                     AEVSARKEVVEAWEALQVELDKVRQF"
     assembly_gap    7375..7474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7475..8115)
                     /locus_tag="PLF67_06500"
     CDS             complement(<7475..8115)
                     /locus_tag="PLF67_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOE89698.1"
                     /translation="MAQALLRFLDNQYVSVDGVEHKFVRGVFGIFGHGCVVGIGEALA
                     SEENALPFYQAKNEQGAVHAAIGFAKEHNRHAMMAVTSSIGPGAMNMVTGAATATANH
                     IPVLLLPGDVFACRQPDPVLQQVEIPHDYTNTANDAFRAVSRLWDRINRPEQLMSAMI
                     NAMRVLADPAETGAVTIALPQDVQGEAYEYPVEFFKKRVHYIERRIPTVGQLDR"
     gene            complement(8136..9167)
                     /gene="iolG"
                     /locus_tag="PLF67_06505"
     CDS             complement(8136..9167)
                     /gene="iolG"
                     /locus_tag="PLF67_06505"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HOE89699.1"
                     /translation="MSVLRIGILGAGRIGKVHAENITRFVKGVEIVGIADPLIDRKTT
                     EWANGLGIKVVTKDSMQLINNPEIDAIMVCSSTDTHADFIMAAANAKKHVFCEKPIDL
                     SVLKGKEAIETAKSKGVKLQLGFNRRFDHNFKRIRELTEDGSIGDVHLIKITSRDPAP
                     PSAAYAAVSGGIFLDMMIHDFDMARYQAGCEVVSVDAHGAVLIDPEIGKAGDVDTAIV
                     TLVFENGALGVIDNSRKAVYGYDQRVEVFGSKGCAIAANDQPNRVTLFGGENVSQDKP
                     LYFFLERYKDAFVDEVAAFVEAIRTDGEVPVDGTDGLEAMVVALAAGKSLKEGRRVTI
                     AEMKKELGL"
     gene            9307..10308
                     /locus_tag="PLF67_06510"
     CDS             9307..10308
                     /locus_tag="PLF67_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE89700.1"
                     /translation="MANIKEVAKLAGVSVSTVSRVFNQQSYVKAETRARILEAIEKTG
                     YVPNRAAREMVAKHATTVGIVMPETFNNFQRQIFSIVARQLEEHSYHTGFYYASTEAE
                     DELKCLNRIKADRLNGIIILHELTQPGFNEYLTVQNIPTVLATFRAPSWNAPAIHISE
                     EEAAAVAVRHLITNGHRNIAVIAGRQFTFSTRRLAGYGNALALANIALDERNIVYSET
                     YSMQSGYEAARHLSERSNTFTAVFAITDELAIGAIRYFQDRGLKIPEDLSVVGFDNIE
                     LSSFVSPRLTTIAQPIEEMGNLAVELLHSMITGKEKSRTDILLPFTLIERESTAQLR"
     gene            complement(10296..11570)
                     /locus_tag="PLF67_06515"
     CDS             complement(10296..11570)
                     /locus_tag="PLF67_06515"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOE89701.1"
                     /translation="MQEIRLTSRYTARNQGAYVHLPIEVAEGIQRIEVSYSYPRFEEI
                     DRRSIEINVIDLGLLDPDGLLCGWSGSERLEVFVSEARSTPGYRRMAIKPGTWHIILG
                     LYKICGEVEIETTIRLIEKKRIRLKGDLHTHTINSDGVFTTAEQAAHAKAAGLDFIAL
                     TDHNNTAQNTEIGAVEGLTVLPGMEYTNYKGHANFLFRDDGLFFENPLSNTFEEAQSV
                     FHAAKARGAVICLNHILDRYCPWNLGWDLPYDLVEVWNGFPKESDLQAIVWWHEQLCA
                     GRKIPAVGGSDNHRIGLGQQIGMPTTVVHALSTGRSDILDALVEGRAYIALTPESPMV
                     EMTIGEKSFGETVPFADDLKGIITITGVKAEDRIVLLSDQGEESRWICERDGFFETRF
                     AVNKRLFYRLELYRFTVSVHLLEVVTNPVYLS"
     gene            complement(11637..>12350)
                     /locus_tag="PLF67_06520"
     CDS             complement(11637..>12350)
                     /locus_tag="PLF67_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89702.1"
                     /translation="AEAKPVWEWLAKLYREGLMDPTSFTGTTGDMRKKMWQSQDIVMD
                     VDWAAWTGLYNNNAVNAGLYPDKVNIVGLPGVLGPDGIYRLEQGGASLWSIPSNAKNP
                     DEAFKILEYMATVEGGLLLSAGIEGHDYVIKDGKLEYTEAGLAHAKDHGAPFPISKNF
                     DLKILGKMNPGVLESVAIGRRDDVEVAPMGFANGQLDARKFYDIMSKWMTECVMGKLS
                     ADAAMKGAENELRTNGIID"
     assembly_gap    12351..12450
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12451..12857)
                     /locus_tag="PLF67_06525"
     CDS             complement(<12451..12857)
                     /locus_tag="PLF67_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89703.1"
                     /translation="MKRTYVLVAMLILLLAGSFLFAQAKPEAEVKVLAAVTGGKDDAE
                     NKLFEEAMEKATGLSITWEKVPSGYDQVLMQKLGAGEAYDLVYINQFQMYSLAKQGAL
                     MDLTDRIASSSVYAQNVDSAELEKIRFEGKFYAG"
     gene            complement(12907..13776)
                     /locus_tag="PLF67_06530"
     CDS             complement(12907..13776)
                     /locus_tag="PLF67_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89704.1"
                     /translation="MKSTFRTYDSLPLFLRMLNVLILFVLVILMGIPMVNVLAVSLST
                     VAKSDSPGLVLFPAPVTLEGYRFIWKNANLAQPFYNTLYVSVVGTALHVLMAALAGYI
                     LAQPNLPFKRVMTTFILLTMTVPYELTMIGIYAVNKSLRLINTYTGLIVNGMISGFSV
                     FLLRNYFLAIPRSLAESARIDGAGELRIFSSVYLRLSTSGLMTIATLELIRRWNNITM
                     TVTLISDMKKWTLPVVLKFILFDQTSVSGSSYVFANAKMAAVVLTAIPLVVLYFFTQS
                     FFSTGVMLGSVKE"
     gene            complement(13781..14668)
                     /locus_tag="PLF67_06535"
     CDS             complement(13781..14668)
                     /locus_tag="PLF67_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE89705.1"
                     /translation="MSGSLLKRIKAFKTFYLMFLPVALYFIVFTYYPFFLGLVMSFQE
                     NRLIGARPFVGLENYRFIVKDSNFIAAIGNSLIIGLWDMVLYFLCSLALALVLNEVRH
                     RTLKQSIQTIAYIPYLFSWSVIGGVWALIFDLQGLVNKVIGLFGVEPIFFLATPDYAR
                     PLIIAMGVWRSMGYFALLFTVSIMSIDQQLFEAARIDGASRMEQIRRIILPGLIPTMK
                     TIIVLLSIGVLTHFDEIYVMVNPANRRLISTLLLYVYETGILNFKTGTASAGAMLVMI
                     GTLVVTISMRRVIGYDKDE"
     gene            complement(14863..15729)
                     /locus_tag="PLF67_06540"
     CDS             complement(14863..15729)
                     /locus_tag="PLF67_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE89706.1"
                     /translation="MDTELNKASRRLSELPLSIEFPDILVEVQWLRIMQKSGHWSIGR
                     HAHSSFEFHLIARGTCEVITDDVTFVAEPGTFYLTGPGVYHTQRSIGTDPLVEYSLDC
                     TFKPQKDNGRGEWGALHLFFKDAKCFPVHDAHGAIALFDKAIEEALCLQPGYPIMVHS
                     LVLSILVSMGRSMGYTSSFLQSKSVSRMELIASYIEDNIYQQITPADIAAFLNLSEKQ
                     VSRIVLSSEGISTKRFITLAKIEEAKTLLVESDLPIGEIAQSLGFATASYFNTVFRNV
                     EGITPGAFRAQI"
     gene            15785..>17834
                     /locus_tag="PLF67_06545"
     CDS             15785..>17834
                     /locus_tag="PLF67_06545"
                     /inference="COORDINATES: protein motif:HMM:NF027792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HOE89707.1"
                     /translation="MIERVHLVFKTHLDIGFTDLAKNTVTRYLEEFIPKAVKVSRQLE
                     AMGAAEQLHWTTGSWLIKRYLEHAQGSELASALDAIEKGVITWHALPFTTHTELMSKE
                     LFTYGLSLSRDLDRRFGHNTIAAKMTDVPGHTVAIVPLLAKAGVTFLHIGVNGGTPVP
                     NLPPVFRWRAETGEEVIVQYDGSYGASGAIEGCPDLLHIENSLDNAGPPSLREVLDTY
                     ERLRKQYPHAEIVASDLSEYARHLTTYRTHLPVVTSEIGDTWIHGVGTDPKKVALLRE
                     LLRFVERNPSLQTDPTVMEPLLLVCEHTWGLDFKKYLADYTSYTVKDLAAARKKDTVG
                     PDAIPKKYAFIENHAQEELKATFGPNDRRKDHRSYSFFTSSHEEQREYINQALQALAP
                     EQRKIVAEGMKSGIRTGSPAARLEADTPVTIGSSTLRFGADGALVSYRRNGKEFAGGE
                     GIGVYRYERFGADEYEAYHHQYNRNFEQGKFWILGDFGKPGLELVNPKVVHRLYPGVL
                     ADLTLHHHENEVMIIASLQRGANVPESAPGRVTIAYFFTLDGILRRITLEWSGKRASR
                     IPEALWLSVGLANTAGGTWRMSKLSTLIPMDDVVSKGSRSLHAVESLHYQSGNEALTI
                     TNLDSPLVAPSKRKLLEFDDRLPSMDGLFHFNLYNNKWNTNFRLWYDEDGQSTLIFTS
                     D"
BASE COUNT         3790 a         5197 c         4803 g         3744 t
ORIGIN      
        1 tccgaactcg atacgctcaa ccggggaggc acgaacctgc ccagccattg cgaccatctc
       61 cgaaccaaag ggatcgatgt cacgaccggc tccctgggcc aggggctttc cgtcggatgc
      121 ggagttgcgt attccctcct ccatgacggg aaacagcagt atgtctacgt ggtcatcgga
      181 gacggggaga gccaggaagg acagatctgg gaagcggcga tgtttgccgc tcattacaag
      241 ctgaagaaca tgatcgtatt caccgattac aacaagcttc agctggacgg caagatcgat
      301 gacgtgatga gcctcgggaa tttggcggaa aaatggagaa gtttcggatg ggatacccgg
      361 caggtggacg gacacgacct gaatgcgatc catgaagccg tcatcaatgc ccaaaaccaa
      421 cagactcgtc cggccatgat catcctcgat acgatcaagg gaaaaggagc cgccttctgc
      481 gaagggctgg aaagcaatca caacatgagc ttctcgatgg aaacggccaa gcaggccatc
      541 gccgaactgg ggtaaaggag acgacatgaa ccaaaaagaa atgcggacgg tatatgcgga
      601 gacgttgatc gaactggctc acgaggattc gagaatcatg gttctggaag ccgatctcat
      661 gcgggcccac ggcacccaga tcttcaagga cgtctttcct cggcgaactg tcggagtcgg
      721 cgtcgccgag gcgaaccttg tcggggtaag cgcgggatta agcgtcgccg ggaagattcc
      781 ctttgcggcg acattcgcca ccttcgcttc ccgacgggcg ttcgaccagt tcttcatctc
      841 tgccaattat gccgggctca atgtgaagct cgtcggaacc gatccgggag tgagtgccgc
      901 tttcaacggc ggaacccaca tgccgtttga agatatcggg ctgatgcgga tgatccccga
      961 cctcgtaatc gtggagccgg ccgatccgac aagcctgaag gaaatcatca ggctttgcgc
     1021 cgcacatcag ggcaacacct accttcgtct tcatcggaag gcccttcccc aaatctatca
     1081 gacaggagaa cgcttcgagc tggggaaagg aaaactcctt catgacggtt acgacatcac
     1141 gctcgttgca agcggagggc tcctcgtgaa tgaagcgcta aaggccggag aactgcttaa
     1201 aaaccaggga atcagcgcag cggtcatcga tatgcacacc atcaagccgc ttgatgagca
     1261 gctgatcctt gagtacgccg aaaagacggg agcgatcgtc acggcggaaa accatcaggt
     1321 cgcagggggt ctcggcgccg ctgtttcgct cttcctgtcg acccggcatc cgatcccgat
     1381 ggaaatggtc ggcattcatg accggttcgg acaggtcgga acccaggact ggctgatgca
     1441 gcattacaaa ttgacggcgg acgagatcgt tcgaaaggct ttcctggcgc ttacgcggaa
     1501 acactagtgc ttgccggatg aagagagaat gggaacgcac atccccccgt cccgaagaac
     1561 aaggacccgg gggtctcggc ggtgcgcttg gcgaaactcg tcgacgacgc ggtcaaacca
     1621 tcgttgaaga tccttgaccg gcttgaggaa caaggatgaa acgcgctcgt cgtccaggtc
     1681 ggtataggca tacaggttga tccgcttcgc ttccaatgcg atcttcgccg ctttgtggta
     1741 gccgagcttg tagtgttcat gaatcgctgc gagcgcctga tccggagatt cgctggaagc
     1801 gagcagatcg gcgaacgccg caccccccac cccctcccgg caggaggcga ccagcaggag
     1861 ggatcctccg tcacgaacgg ccagcgcccc gttgtcgagg gctttctgcg cttgatacag
     1921 gttgatgtcc atcggatggt tcgccacggt gatcacgatg tccattggtg aagcgatcgg
     1981 cgttccgaag gtttcttcag ctaccgggat gcactcaagg aaactctccg tcagcgtgcc
     2041 tccggcgaga tggatcggtc tatgatcctt gtcaaggacg ctcatcagcg aaaagatcgg
     2101 ctgcttgacg aactccacca cctccaggaa atcctcgtgc accgggttgc cctccagagc
     2161 aagggtgtgc gcggcgctgc cgagggcgag gcggtggttg gcttcgatcg tctcgtagcc
     2221 ggcgattccg ggcatgatcg ccttccggcc tccggtatag ccggcaaaat aatggggttc
     2281 gacgctcccg gtgacaacca cccgatcgac ctcgaaaaga agcgaattca acgctacggg
     2341 tgttcctctg ctcgtcgttc cgagataggt caaccggctc cggtcggtgg cgacgtggct
     2401 gacgatccgg ttgttcaggc gtcgggccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnc ggctccggtc ggtggcgacg tggctgacga tccggttgtt caggcgtcgg
     2581 gccagccccc tgccgagcag ctgctcgaac tccgattcca gagcgggccg atgagctccg
     2641 gtggcgatga gaatcgttaa ttgatcatcc tgtattcccg cctcttcaac gtacggcagc
     2701 aaggcgctca tcatctcctt cgtcggcgtc ggccgcgtcg aatcgttgat gatgatcaac
     2761 acccggctgg cagatcgaag aaaatcggca aacccggtcc ccagcgcctc tttaaacaga
     2821 tgctttcgag ccctcagtcc gccttccttc ggatcccgta ccgatacccc aagggctttg
     2881 ggcgctttga gaacaagatg ttccccgctt cggtacggca atacgataga atccatccgt
     2941 tacgccttca tcgcctcaag caagggcagc ctcttgcctg aaaggaattg gatcatcgct
     3001 cctccggcag tgcacacgta cgagtacccg tcaaggccgg tatagcgcgc ggcggcggtg
     3061 atcgtgtctc cgcctccgat gaccgaatat gccgtgctgt tgctgaccgc ccgaagcagc
     3121 tcccgtgtgc cattctccca ccgctcatcc tcatacaccc ccgccggtcc gttgacaaac
     3181 agggtgttcg cccgggcgat gtgctccgta tacagggcga tcgttttcgc tccgatgtcg
     3241 agatagaagc ggtcatccga ggcttcaacc tcctcatggg tgcattccgc ccgctctccg
     3301 tctttttcaa aggcgaagtc aagggggaag aacaccttct tcggaaactc ctcaagcagc
     3361 ttcttcgccg gagcgatgaa ccggatgaaa tccttctccg caaggaacgc ttcggtagcg
     3421 ctccccagct ttactccgga agcgagcgcg atcacatgac cggtcaggcc gccggtgaga
     3481 accgcatcgg cgcttccggt ggagaggaca tgatccatca tgccgaacgc atcgctgatc
     3541 ttcccccctc cgagcaggaa gaccctcgga tgggccgtcc cttcggagat ccggctcaag
     3601 gcggagtact cgtccatcaa ttgccggccg gcataggtgg gcagaaactc ctggaaggcg
     3661 accatcgacg gggtgctgcg atgggcggcg gagaacgcgt cgttcacgta gagatcgaaa
     3721 tgatccttca gcgaccggat cagataggtc gattgcatct cggtcggggt gagcttcacc
     3781 tccttctcga aggcggtgat ctcctcgctc aggtagcgga catttcccag caggatcgcc
     3841 tcgctttcgg aaagcgattc aatcgcttcc tgagcggcca gaccgcatac gtcatcgatg
     3901 taggacacgg gaaagccggt aaggttctgc aggatctcgg cgtgttcggc catcggaatg
     3961 atgttccggt aatccatcgt atcaccctgg tgggcgatga tcgcgacctt ggcgcccccc
     4021 tcaagcagca gcttgagggt gaccgcggcc ttctcaaggc ggttcgtgtt gacgatcctc
     4081 ttcgtctttt gatcgatcgg actgttgatg tccggtctga acagcacccg tttggaacgg
     4141 acatccaccc cttcaatgct cttcatcatc gattccacct gccgatgcct aagtattcat
     4201 tcgtcttgga agttccctct tcccgcgttg tctgcagctt cagagcagag cggatcgcat
     4261 cgatcgattc ggggatcacc accgcttcct ggggaatgtt gatggcgaac atgatgtcct
     4321 tcccgctctt gaccaccgaa tccttccata cggcgatctc atacatgtcg ccccgcggat
     4381 tgcccagatc gcgggcgtat cggaagaggc tggcgttgcc caaaaatcct tcatcgatcg
     4441 agacgacccg aatcctcgga tgggctttca gatgggcaac cgcttcttcg accgagagat
     4501 ccttcttcgg cgtgagtacc accgtgatga tatgcccgtg ggtcaccggg gtatgcacaa
     4561 gaatgcccgt cgcctcgata tgaggcatga tcgtcatcaa atccaccgcc tgatggctcg
     4621 gagccttgtc catctgaagg gcgttcgtca ggccgcggtg ataatcgccg gggtcggcga
     4681 cccgccgaat gatcgtgatg gcgaccttct cgactccgac cgcccggtcg aagcaatcga
     4741 ccgcccggat caggccggtg gtgttgcagc tcgtcagctt cagatacgac tggcccactc
     4801 cctgctcata gtttgcatag ccgtgaaaga acacgtcggc gatggcgttc ttttcaccgc
     4861 cctggaaaac cgcttttttc ctatgcttct cgtacagctc cttgttcttc agcccgacgc
     4921 ccgcgctggt ggcgtccagg atgatgtcgc acttttgaac cagctcttca aggcttcccg
     4981 tgaccggcac cccgagctcc tccagaaccc ctcgattcgc aggatttgcc aaatagaaat
     5041 caaagggcat tccccgttcc ctgagcgctc tgatcgagag ggtcggcccc acatccgcca
     5101 ccccgacaag ctccatatcc ccttgaaggg caactccatc ggccaaccgc tgtccgatca
     5161 cgccatatcc gactactccg actttcacac gtttgctcat tgtcctacct ttatcaaatc
     5221 tttgcgatgt cacggatgta cttccgcgcc ttcattgcat actcaagcgg attggcgatc
     5281 ttcggatcct gttccgcttc caccacccac cagccctcat acccgttggc cagcagatgc
     5341 tcgacgatcg gcctgaaatc gatcattccg tcccccggta ccgtgaacac cccttccaga
     5401 acccctttga gaaacgacca cttctctttg atcgcctgtt ggcgttttgc ggcccgcaca
     5461 tccttcaggt ggacgtgcca aatccggtcg aaccacttcc taagcggaac catcgggtct
     5521 tcgccgctga agacgaggtg gccggtgtcg tagagcaggc ctaccaagtc cgcgtcggtt
     5581 tcctccatca ggcggtcgat ctccccgttg gtctggacgc cggtgcccat gtggtggtgg
     5641 taggtcagcc gaaggccgaa ctcccgcgct ttcttgccga gcttgtcgag cccggccgca
     5701 agcagctccc actgtcgctc gctgttgacc ggcttgccgt caaaaaccgg aatgtccgga
     5761 tcgccttgaa tcgagttgcc ctgttccgcc accccgacga tcttggcgcc catcgcctga
     5821 aggaacgtgc aatgctcgac aaatgcccgt tcaacctcct catagggcct ggtcgtcagg
     5881 tatgtgctga accaggcgtt gcagatccga aggccccgca gctcgagcgc cgggcgcaga
     5941 accgcgggat ctttcggata tttgtttccc acctcgcttc cttcgaagcc ggccagcgcc
     6001 atctcgctga cgcactgttc aaagggaatc tcgccgccga gctggggcag atcatcattc
     6061 gtccagccga tcggggcgat tgccagcttg atccgagtgt tgctcattgc tcctcctaaa
     6121 attgccgaac cttatcaagt tcgacttgta acgcttccca ggcttcaacg acctctttcc
     6181 gagcggagac ctccgccgtg ccgacccgcc accacccttc gtaattcccg gtcatcgact
     6241 tgggcgcgac gagacagtgg atcaccgtgg agacctcttc cttcagcgcc tgctgtaccg
     6301 cctcggcaag gccttcgatc gtcgtcgccc ggaggccaac cgcaccccag ctttgggcat
     6361 tcagggcgta atcgacgacg aggctctgcc cgctcaagcg gccgctttgc tcctcgcgct
     6421 tccgccactc attgccgtaa cggacgatgc cctggcttgt ctgaagatta tcgatgcagt
     6481 ggaagcccgc gttgtcgagg accacgacga tgattttctt cccctcctgg attgacgtga
     6541 gcaattcggt gtggagcatc gtataggcgc cgtcgccgac gagggcgacc acctcccgtt
     6601 cgggatgggc gatcttcgct ccgagcgccg ccgcgatctc ataccccatc gtggagaacg
     6661 catattccat atgataggtg ccgggaaccc ttgtccgcca cacccgctgc atatcgctgg
     6721 ggatcgagcc ggaagcggag aggacgatcg catcgggcgg cagcagacgg tcgttcagtt
     6781 ctccgagaac tctcgcttgg gagagcgtcc cttcaagctc ctcatggtag agctgctcga
     6841 cctcccgatc ccagaccgct ttctcttccc ggatgcggtt cccccattcg cttcgatagg
     6901 atctcaattc ggggtggcgg acgatcgcct ccagcgtcag tttcgcatcg gcgatgatcg
     6961 gttccgcatt gagtttgtat ccgtcgaacg aagcgacgtt gattgaaaca aaggaggccc
     7021 gagggttccg gaagagccac ttggaacacg tggtgaagtc gccgaaccgg gtgcccaccc
     7081 cgatgatcag atccgcctcc ttggctaacc gattcgccgc aagcgaaccg gtggtgccga
     7141 tccccgagag attataggga tgatcccaga cgatcgtccc ctttcccgcc tgcgtttccc
     7201 cgaacggaat atggaaggtc tcacagaagg aggccagttc cgctcccgca gcgctgtagc
     7261 ggatgccgcc tccggcgatc acaagcggtt tcttccccgt cttgatgagc ttcgccaagc
     7321 ggtcgagctg tcctacggtg gggattcgcc gttcgatgta gtggactcgc ttctnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggtcg agctgtccta cggtggggat
     7501 tcgccgttcg atgtagtgga ctcgcttctt aaagaactcg acgggatact cgtacgcctc
     7561 tccttggaca tcctggggca acgcgattgt caccgccccc gtttccgccg ggtcggcaag
     7621 aacccgcatc gcattgatca tagcgctcat cagctgctcc ggccggttga tccggtccca
     7681 cagcctgctc accgcccgaa acgcgtcgtt ggcggtgttg gtgtagtcgt ggggaatctc
     7741 gacctgctgc agcacgggat cgggctggcg gcaggcgaac acatctccgg gcagaaggag
     7801 aacggggata tggttcgccg tggccgtggc ggccccggtc accatgttca tcgcccccgg
     7861 tccgatcgag gaggttaccg ccatcatcgc atggcggttg tgttccttgg caaacccgat
     7921 cgccgcatgg acggcaccct gctcgttttt cgcctgatag aacggaagtg cgttttcttc
     7981 gcttgccagc gcttcgccga tcccgacgac gcatccgtgt ccgaagatcc cgaacactcc
     8041 gcgcacgaac ttgtgctcga ctccatccac gctcacatat tggttgtcca gaaagcgcag
     8101 caacgcctgc gccatcgtca gtcggatcgt tttgttcaaa gccccagctc ctttttcatc
     8161 tcggcgatcg tcacccggcg cccctctttg aggctcttgc cggcggcaag ggcgacgacc
     8221 atcgcttcaa gtccgtcggt tccgtcaacc ggaacctcgc catccgtccg aatcgcctcg
     8281 acaaaggcgg caacctcgtc gacgaacgca tccttgtacc gttccagaaa gaaatacagg
     8341 ggcttgtcct ggctcacgtt ctctccacca aagagcgtca cccggttcgg ctggtcgttc
     8401 gcggcgatgg cgcatccttt ggaaccgaac acctccaccc gctggtcata gccgtagacc
     8461 gcttttcggg agttgtcgat gactccgagg gctccattct caaagacgag ggtgacgatc
     8521 gcggtgtcca catcgcccgc cttgccgatc tcgggatcga tcagaaccgc cccgtgggca
     8581 tccaccgaaa ccacctcgca tcccgcctga tatcgggcca tgtcgaagtc gtggatcatc
     8641 atgtccagga aaatgcctcc cgagaccgcg gcgtaggcgg cgctcggagg cgcgggatcg
     8701 cggctggtga tcttgatgag gtggacatcc ccgatgcttc cgtcttcagt gagctcgcgg
     8761 atccgcttga agttatggtc gaaccgccgg ttgaacccaa gctgaagctt cacgcccttg
     8821 ctcttggccg tttcgatcgc ctcctttccc ttcagcacgc tgaggtcgat cggtttttcg
     8881 caaaagacgt gcttcttcgc attggccgcc gccatgatga aatcggcatg ggtgtccgtc
     8941 gagctgcaca ccatgatcgc atcgatttca ggattgttga tgagctgcat cgaatccttg
     9001 gtgacgacct tgatccccag cccgttcgcc cattccgtcg tcttccgatc gatcagggga
     9061 tcggcaatcc cgacgatctc cacgcccttc acgaacctcg tgatgttctc ggcgtggact
     9121 tttccgattc gccccgcccc caagataccg atacgcaata cagacatgtt tctttccttt
     9181 ttagtgatat gaaaacgttc ccagatatgt gtagtataca ccaaagaacc ccaatgtaaa
     9241 gtcatattgt actcattggg gtttcagggt atgtagtata tactggaagg aagtggtata
     9301 gaggtcatgg cgaatatcaa agaagtggcg aagctggcgg gcgtttcggt atcaaccgtt
     9361 tcacgggtgt tcaaccaaca gtcatatgtg aaagcggaga cccgggcgag gattctcgag
     9421 gcgatcgaga aaaccgggta tgtaccgaac cgcgcagccc gggagatggt ggccaagcac
     9481 gccaccacgg tcggaatcgt catgccggag accttcaaca acttccaacg ccagatcttc
     9541 agcatcgtcg cccgccaatt ggaggagcac tcctaccaca ccggcttcta ctacgcttca
     9601 accgaagcgg aagacgagct gaagtgcctg aatcggatca aggcggatcg cctcaacgga
     9661 atcatcattc ttcacgagct tacccagccc ggattcaatg aatacctcac tgtccaaaac
     9721 atcccgacgg ttcttgcgac cttcagggcg ccgtcctgga acgccccggc gattcacatc
     9781 agcgaggaag aagccgccgc cgtcgcggtt cggcacctga tcaccaacgg gcatcgcaac
     9841 atcgccgtga tcgcaggacg ccagttcacc ttttccaccc ggcgcctcgc cggatatggg
     9901 aatgcgctcg ctctggcgaa catcgcattg gacgagcgga atatcgtcta ctccgagacc
     9961 tacagcatgc aaagcggcta tgaggccgcc cggcatctca gcgagcggag caacaccttt
    10021 accgcggtgt tcgcgatcac cgatgagctg gcgatcgggg cgatccgata ctttcaggat
    10081 cgagggttga aaatccctga agatctttcc gtcgtgggct tcgacaacat cgagctttca
    10141 tcctttgtct ccccccgtct gacgacgatc gcccagccga tcgaggagat gggtaacctt
    10201 gccgtcgaac tgctccattc gatgatcacc ggcaaggaaa aatcccggac ggacattctg
    10261 cttcccttca ccctcatcga acgggaatca accgctcagc tcaggtagac gggatttgta
    10321 accacttcca gcaggtgaac ggatacggtg aagcggtaga gttcaaggcg atagaacagg
    10381 cgtttgttca ctgcaaaccg ggtttcaaag aacccgtccc gttcacaaat ccaccggctc
    10441 tcttcgccct ggtcgctcaa gaggacgatc cgatcttcgg ctttcactcc ggtgatcgtg
    10501 atgatccctt taaggtcatc ggcaaacggc acggtctcgc caaaactctt ttcgccgatc
    10561 gtcatctcca ccatcggcga ttcgggggtc agagcgatgt aagcccgtcc ctcgaccaat
    10621 gcatcaagga tatcgcttct gccggtcgac aatgcgtgga cgactgtcgt gggcatgccg
    10681 atctgctgtc ccagaccgat gcggtggttg tccgagccgc cgacggcggg gatcttcctt
    10741 ccggcacaga gctgctcatg ccaccagacg atcgcctgaa gatcgctttc cttgggaaac
    10801 ccgttccaga cttcaaccag atcgtaggga agatcccagc ccagattcca cgggcaatac
    10861 cggtcaagaa tatgattcag gcagatgacc gcaccccgtg ctttcgctgc gtgaaagacg
    10921 gattgggcct cttcaaacgt attggagaga gggttttcaa aaaagagacc atcgtcccgg
    10981 aaaaggaaat tcgcatggcc tttgtagttg gtgtactcca tccccggaag aaccgtcagc
    11041 ccctccaccg ctccgatctc ggtgttctgc gccgtgttgt tgtggtcggt gagcgcgatg
    11101 aaatcaagac cggccgcttt cgcatgggct gcctgctcgg ccgtcgtgaa cactccgtcg
    11161 ctgttgatcg tatgggtatg cagatccccc ttgagccgga tccgcttttt ttcaatcagc
    11221 cggatggtgg tctcgatttc gacttcgccg caaatcttat acaatccgag gatgatgtgc
    11281 cacgtgcccg gcttgatcgc catccgccta tagccgggag tcgaacgtgc ttccgaaacg
    11341 aagacttcca gccgttcgct gcccgaccat ccacagagga gcccatccgg gtccaagagc
    11401 cccaagtcga taacattgat ctcaatgctg cggcggtcga tctcttcgaa tcgggggtag
    11461 gaataggaaa cttcgatccg ctgaatgccc tctgcgacct caatcggcag atgcacatac
    11521 gcgccctgat tacgggctgt atatcgggaa gtaagacgga tttcctgcat cgcttgtcct
    11581 tggatgaaga tgaggcgcag ctaaccatgt gccgcgcctc atcatctctc acatcattag
    11641 tcgatgatgc cgttcgttct cagttcgttt tcggctccct tcatcgccgc atcggcgctg
    11701 agtttgccca tcacgcattc ggtcatccat ttactcatga tatcgtagaa tttccgtgcg
    11761 tcaagctgtc cattggcgaa tcccatcggg gcgacctcga catcgtctcg acgcccgatc
    11821 gccacggatt caagaacgcc ggggttcatc ttgcccaaga tcttgagatc gaagttcttg
    11881 gagatcggga acggagctcc gtgatccttg gcatgggcga gacccgcttc ggtatactcg
    11941 agcttgccgt ccttgatgac atagtcatgg ccttcgattc ccgccgaaag gagcagtccg
    12001 ccttccacgg tcgccatgta ctccaaaatc ttgaatgcct cgtcgggatt cttggcatta
    12061 ctcggaatcg accatagcga ggctccgccc tgctcgaggc gatagatccc atcgggaccg
    12121 agcacgccgg gaagaccgac gatattgacc ttgtccggat acaagcccgc attgactgcg
    12181 ttgttgttgt acaatccggt ccaagcggcc cagtcgacat ccatcacgat gtcctgactc
    12241 tgccacatct tctttctcat gtctccggtc gtcccggtga aggaggtggg atccatcaat
    12301 ccttcacgat agagcttcgc aagccattcc caaaccggtt tggcctccgc nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccggcgtaga acttgccttc aaatcggatt
    12481 ttctccaatt cggcggaatc cacgttctgc gcatacacgg aagagctcgc gatccgatcc
    12541 gtcaaatcca tcagcgcgcc ttgcttggca agactgtaca tctggaattg gttgatgtag
    12601 accagatcgt atgcttcacc ggcaccgagt ttctgcatca gcacctggtc atatcccgaa
    12661 gggacttttt cccacgtgat ggacaggccg gtcgctttct ccatcgcctc ctcaaacagc
    12721 ttgttttcgg catcgtcttt tccaccggtg acggccgcca gcactttcac ttcggcttcc
    12781 ggttttgcct gtgcaaacag aaacgaacct gccagcaaga gtatgagcat tgccaccaac
    12841 acatacgtac gtttcattgt tatcctccta tctggatagt acgattgatc gttcggccat
    12901 cgaggttcac tccttgaccg aacccagcat caccccggtc gagaaaaacg actgggtaaa
    12961 gaaatacaac acgacaagag ggatcgcggt cagaacgacc gcagccatct tggcatttgc
    13021 gaacacatag ctcgaaccgc tgaccgaagt ctggtcgaac aggatgaact tgagaaccac
    13081 cggaagagtc catttcttca tgtcgctgat cagggtcacg gtcatcgtga tgttgttcca
    13141 gcgcctgatc agttccaatg tagcgatcgt catcagcccg gaggtcgaga gcctgaggta
    13201 cacggaagaa aagatccgca gctcacccgc tccgtcgatc cgggcggatt cggcgagcga
    13261 gcgcggaatc gcgagaaaat agtttctcag caggaagacg gaaaacccgc tgatcatccc
    13321 gttgacgatc aggccggtgt aggtgttgat cagcctcagg ctcttgttga cggcgtagat
    13381 gccgatcatg gtcagctcat acggaacggt catcgtgagc aggatgaacg tggtcatcac
    13441 ccgtttgaag ggaagattcg gctgagcgag gatatatccg gcaagggcgg ccatcagcac
    13501 gtgaagagcg gtgccgacca ccgagacata aagggtgttg tagaacggtt gagccagatt
    13561 ggcgtttttc cagatgaatc gatacccctc cagcgtcaca ggggcgggaa acaatacaag
    13621 accgggcgaa tcgcttttgg cgaccgtcga aagggacacc gccagcacgt tgaccatcgg
    13681 aatgcccatc aggatgacga gcacgaacag gatgagcaca ttgagcatcc gaaggaaaag
    13741 cggcaaggaa tcatatgtcc gaaaggtgga tttcatgccg ctactcatcc ttgtcgtatc
    13801 ctatgaccct gcgcatcgaa atggtgacca caagcgttcc gatcatgacc agcatcgccc
    13861 ccgccgatgc cgttccggtt ttaaagttta aaattcccgt ctcatagaca tagagcagca
    13921 acgtcgaaat cagacgccga ttcgccgggt tcaccatcac gtagatctcg tcaaagtggg
    13981 tcagaacgcc gatggacagc agcacgatga ttgttttcat cgtcggaatc agaccgggaa
    14041 gaatgatcct tcgtatctgc tccatccggg aggcgccgtc gatccgggca gcctcgaaga
    14101 gctgctgatc gatgctcatg atcgaaacgg tgaagagcag cgcgaaatac cccatcgacc
    14161 gccacacgcc catcgcgatg atcaggggac gggcgtaatc gggggtggcc agaaagaaga
    14221 tcggttcgac accgaatagc ccgatgacct tgtttaccag cccctgaaga tcgaagatca
    14281 acgcccagac tcctccgatg acggaccagc tgaacagata cggaatatag gcgatcgtct
    14341 ggatcgattg tttcagcgtg cggtgtcgca cttcattcaa taccaacgca agggcgagcg
    14401 aacagaggaa atacagcacc atgtcccaca gtccgatgat cagggagttt ccgatcgccg
    14461 cgatgaagtt ggaatccttt acgatgaagc gatagttttc caacccgaca aagggccggg
    14521 cgccgatcag gcggttttcc tgaaagctca tcaccaagcc gaggaagaaa ggatagtatg
    14581 tgaaaacgat gaaatacagc gctaccggaa gaaacatgag gtagaacgtc ttaaaggcct
    14641 tgattcgttt cagcagtgat ccactcatac ggaacccttt tataataacg ttttcacacc
    14701 gttcatcagc agtgtatcat ggattataat ttttggacat agtaattatc gcttagtttt
    14761 gctattttgg taaaataccc ttattagagg cattttacat taatttaacg tatttttgtt
    14821 tataaatttt cggacatgtt gtatgataag ggaaggagtc ttttagattt gtgcgcggaa
    14881 tgccccgggt gttattcctt caacattgcg aaacacggta ttgaagtagc ttgccgtggc
    14941 aaaccccaag gattgagcga tttcaccgat cggtagatcc gactcaacca gaagcgtctt
    15001 ggcttcttcg atctttgcga gcgtgatgaa ccgtttggtg gagatgcctt cagaggaaag
    15061 gacgatccgg ctcacctgct tttcgctcag attgaggaat gccgcgatgt cggccggagt
    15121 gatctgctga tagatgttgt cttcgatgta gctggcgatc aactccatcc ggctcacact
    15181 ttttgattga aggaaggatg aggtgtagcc catgctgcga cccatggata cgaggattga
    15241 gagaacgagc gaatggacca taatcggata gccgggctgc aggcacagag cttcctcaat
    15301 cgctttatcg aacagagcga tcgccccgtg cgcatcgtgt acgggaaagc acttggcatc
    15361 tttaaagaag agatgcaatg caccccactc gccccgtcca ttgtcttttt gaggcttgaa
    15421 cgtgcaatca aggctatact cgaccaaagg atccgttccg atggagcgct gggtgtggta
    15481 cacccccgga ccggtgaggt agaaggttcc cggttcggcg acaaaggtga catcatcggt
    15541 gatgacctcg catgttcccc gagcgatcaa atgaaactca aaggaggagt gggcatggcg
    15601 tccaatggac caatgcccgg acttttgcat gattcgtaac cattgcacct cgacgagaat
    15661 atccggaaat tcaatgctca gaggcaattc ggaaagccgc cttgaggctt tgttcagttc
    15721 agtgtccaca tgtcaagtat gccaagagca cacccgtgat gcaagcgaaa ggagacggta
    15781 aacgatgata gagcgagtac atctggtctt caagactcat ttggacatcg gtttcaccga
    15841 tttggccaag aataccgtaa ctcgatacct tgaggaattc attccgaaag cggtcaaggt
    15901 ctctaggcag ctggaagcga tgggggcggc ggagcagctg cactggacaa ccggttcctg
    15961 gctgatcaag agatatcttg agcatgccca agggagtgaa ctcgcttcag cgctcgatgc
    16021 gatcgagaaa ggggtgatca cctggcatgc cctccccttc acaacccata cggagctgat
    16081 gagcaaagaa cttttcacct atggcctttc gctttcccgg gatctggacc gccgcttcgg
    16141 ccacaacacc atcgcggcga agatgacgga tgttcccggc cacaccgtcg cgatcgtgcc
    16201 cctcctggcg aaagccgggg tgacgttcct tcacatcggg gtgaacggag gaaccccggt
    16261 gccgaacctg ccgccggtct tccggtggcg ggcggaaacc ggggaagagg tcatcgtcca
    16321 gtatgacggc tcatacggcg cctccggagc gatcgagggg tgtcccgacc tcctgcacat
    16381 cgaaaacagt ctggacaatg ccggtccccc atccctccgg gaggtattgg acacctatga
    16441 acggctaaga aaacaatacc cccacgcgga gatcgtcgcc agcgatctga gcgaatatgc
    16501 ccggcatctc actacgtatc gcacgcatct gccggtcgtc acctcggaga tcggagacac
    16561 ctggatccac ggggtcggaa cggatcccaa gaaagtagcg ctgctgaggg agctccttcg
    16621 ctttgttgaa cgaaacccct ccctgcagac cgatccgacc gtcatggagc cccttcttct
    16681 ggtgtgcgag catacctggg ggctcgactt taaaaaatat ctggccgact acaccagcta
    16741 caccgtcaag gatcttgccg cggcccggaa gaaagacacg gtcggccctg atgcgatacc
    16801 gaagaaatat gcgttcatcg aaaaccatgc acaagaggag ctcaaagcga ccttcggccc
    16861 gaacgatcga agaaaagacc accggagcta ctccttcttc acgtcaagcc acgaggagca
    16921 gcgggagtac atcaaccaag cccttcaggc tcttgctccg gaacaaagga agatcgtcgc
    16981 agaaggaatg aagagcggaa tccggacagg aagtccggcc gcccgccttg aagcggacac
    17041 cccggtcacg atcggctctt caacgctccg cttcggagcg gacggggcgc tcgtctccta
    17101 tcggagaaac gggaaggagt tcgccggagg cgaaggaatc ggggtctatc gctacgaacg
    17161 gttcggggcg gacgagtatg aggcatacca tcatcaatac aacaggaatt tcgagcaggg
    17221 aaagttctgg atcctcggag atttcggaaa acccggcttg gagcttgtga atccgaaggt
    17281 ggttcatcgc ctctatcccg gggtgctcgc tgatctcacc ctccatcatc acgaaaacga
    17341 ggtgatgatc atcgcctccc tccaaagagg agcgaatgtc ccggaaagcg cccccgggcg
    17401 ggtaacgatt gcctatttct tcaccttgga tggcatctta cgccgcatca ccttggagtg
    17461 gagcggaaaa cgggccagcc ggattcccga agcgctctgg ctctccgtcg ggctggcgaa
    17521 caccgcgggg ggaacgtggc gaatgagcaa gctctccacg ctgattccca tggatgacgt
    17581 cgtctccaag ggctcaagat ccctccatgc ggtcgaaagc ctgcactacc aaagcggaaa
    17641 cgaagctctt acgatcacca atttggactc cccgctggtc gccccctcga aacggaagct
    17701 gctcgagttc gatgatcggc ttccttcgat ggacgggctc ttccatttca acctatacaa
    17761 caacaaatgg aataccaact tccgcctctg gtatgacgaa gacggccagt ccacgctgat
    17821 cttcacttcg gatc
//
LOCUS       DAOSLI010000039        17258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3507_length_17258_cov_25.955066, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000039 DAOSLI010000000
VERSION     DAOSLI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 17258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17258
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3507_length_17258_cov_25.955066"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="PLF67_06550"
     CDS             complement(<1..1824)
                     /locus_tag="PLF67_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOE89708.1"
                     /translation="MKGSFLHLVSKYAVITIVLVVAITGFFLYHALFLTLDADYTSLL
                     AKDEMNSTYQGGVATRTPIEKPPIDEVEELDEPTEFPSSYMLLIESPTLYTAEALTSI
                     NEVLDALSESPYLASRFSLFDFVTLEKRGTRLMSVPFASSSWTDEEAALLKERVQSDP
                     MVKNYLVSEDLHGLFINFESVDLTPQMEAELQEILEPLVKQGMSVSINGAAILTNVLM
                     RYLSRDLAIILTLCLLAILVVYYLSFRAKRSVLLPFSMSAMGIIWTFGTMRLLGYSLT
                     IVNIITPAMVLNLGSSYAIHVISEYFAGHEIGRGVIASTRKILRTILFACLTTVIGFL
                     SLLTSKTGALKEFGISVAIGITYCAILAATYLPAVLSLVRKPKVHHIKTYRRGYLASI
                     VNGINVLVGRYWMVFVVLWFIIIAGFGLTRDKISVNTNYMSYLPKRDPFTISSRHFAR
                     TMSGNTPYYITIDAPSGATSFFLEPGNLAQVYAFEEKVKKENNDIRQSLSFASYVAFA
                     NEVYSNERSIPSSKGLLNLLSRMVLLLSKQGKQDLESVISSDGNTLTIILQNYDSIEG
                     EFGTIGSAERIEDTILDNLQLLPGGTIVTLDGEPHRALHFSN"
     gene            complement(1834..>2209)
                     /locus_tag="PLF67_06555"
     CDS             complement(1834..>2209)
                     /locus_tag="PLF67_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOE89709.1"
                     /translation="ILDPTRYRTIEELGKPPRNEYTRLFIARGRRDGLDKRNLVDYLV
                     SRVGAEYRDIQEVTIQDDHSYISAPNQIAEQILTAFAKTSGPAIISRAKPDNPNGKNL
                     SARRKGERNRNQRSYRKNRNRR"
     assembly_gap    2210..2309
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2310..3644)
                     /locus_tag="PLF67_06560"
     CDS             complement(<2310..3644)
                     /locus_tag="PLF67_06560"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE89710.1"
                     /translation="MTNTPTFADLGLSAQTIQAIENKGFIHPTGIQATCIPLLLKDRV
                     DVIGQAQTGTGKTAAFGLPILELVEEQLNAVQALILAPTRELALQSAEEILSLKGGRN
                     LEIAAVYGGAAIVNQLRQLKKGVHVVVGTPGRILDHLRRGSLVLDQLKFLVLDEADEM
                     LDMGFLEEIEAIMVRTPKEKRTLCFSATMPDPIRRLAERYMDKPQVVRIADETLTTDL
                     TRQLFVEVRESDKFEALTRFIDIEEKFYGIVFCRTKLQCDEVGQKLIARGYHAEALHG
                     DLSQKQRESILNRMRTRQLAIIVATDVAARGIDISALTHVINYNLPEDPEAYVHRIGR
                     TGRAGKQGVAITFVAQREFKRLQFIRRTIQSDIQRTAIPPIEAIIATKRKQIVERLKN
                     LEEPNDSFLNLALEVAGSKLPETVLASVLANLYKEILDPTRYRTIEELGKPPR"
     gene            complement(3709..4743)
                     /locus_tag="PLF67_06565"
     CDS             complement(3709..4743)
                     /locus_tag="PLF67_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOE89711.1"
                     /translation="MQHEVTNNQPLLGKNGRIIEEGWARRPVWKYDRSMVKGTAFRIK
                     EWEYYAIVNQDEQWATAVTFSDLGYAGVFAIAYIDLKTGRVSQTDAIAPLTLGKLGIP
                     ATSTEDHELAWANKHIRIALVKRGEERHIMYACPSLDLGDGIIGLDVDITLKQPSSLE
                     SMTIATSWKKNRTCFYHNEKVNCLGATGTIRRGTALQRLPLGSTFGVLDWGRGKWTYR
                     NTWYWASCSGTVEQVPFGLNLGYGFTDRSPASENVIIYDSVVHKLREVTFEYGSYLEE
                     WTIRDDEGRLDLVFQPVTDRSSHFNLLLIASEQHQIFGHFSGRCTLDNGNTIVVDKIA
                     GFAEEVANRW"
     gene            complement(4813..6351)
                     /locus_tag="PLF67_06570"
     CDS             complement(4813..6351)
                     /locus_tag="PLF67_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOE89712.1"
                     /translation="MSGNDKNDVKQKKVLSFKGGKKQQESKAKAKPRVTVGWILGMAV
                     LLIIGASFIIGPTLGAVLGKRNQGNLVFGTYGKEDISYAYNNYFFDQVQHYADQYKGS
                     QSDPTQTLYQIWKSAYDSTVVYTAVGQLAKQAGIQVNTDVLNRAIIDSGAYHKDGTFD
                     VDTYQKATTERKSAIESSIRRNLPYRMVMTDVSTVLASEAEREYVGNMASKVRDFSYL
                     LFDSSLYPDSEAIAYALEHKQLFENIELSIISLGSLEDAEEVASSLQSGLYTFEEAAR
                     EHSLDMYAQADGYIGTIPFFAIKPNFRYEDEAAALLGADSGSIVGPFETGGGWAIYHV
                     STAAEEADVADENTRNLVKAYIGSNEEELMDSYLSNLVTEVSEYAKKTGLVEAALEYD
                     LDLVEVMPTGYNVGKSSFLNNFSYTDPTGMLASYATAEVQTELFTSAEHTLLEPMKTG
                     GAYLLIEVGEDSTDEGMGSYTMMFYDYLAGSQSQENLAQTLYASDLHKDNFLTTFLRV
                     VLGQ"
     gene            6493..6565
                     /locus_tag="PLF67_06575"
     tRNA            6493..6565
                     /locus_tag="PLF67_06575"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6526..6528,aa:Pro,seq:cgg)
     gene            complement(6972..9095)
                     /locus_tag="PLF67_06580"
     CDS             complement(6972..9095)
                     /locus_tag="PLF67_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE89713.1"
                     /translation="MTRLKGRKNSVTPIWILKVFIPIASIFLLTMIIMALLFSGMFTN
                     VAQSLIVDDFLASLKMISTYYRQMRFNTVPILDDLSDAQEIRDYLLQNLPKDKAMLGA
                     YLRLENTVARNSYIHSIYLYNSEYGFFSSLSGAEVPGELSDSTLLEFLATHPKNKHLY
                     QRRSVFTDKTIPLSLPKKPNTPTNLYTICNISYGEDGNVQSGIIMNLSETLARSLLAD
                     DEGTSKNFYMIDEDSYIISHPDPDQFGRKATSLPLLSQIASFDGPKGGQNIKDDEGEQ
                     FLACWYDIGEMNWRLIFILPMSHLRAPLNTLRKNLVLVFIGLTLIASLMLVWESKRIN
                     NQLTRENRFIDYLKGGLNAEALPLYEGQTFFIAVFLRQTKKPESELLQKNRFISFASQ
                     YLRFNGKNSFLLTMDQGQHVYLTLYEPVKVISRFAKLLIDLGDLFDEQLSIFYSSDRV
                     PLEDLPQRYEHMRRRLLSSSLTEIGFISEIEQEDTKEVNLFLTETTDMEKALSQRSAE
                     AYAKSVDTLITNLKRQDDFELFSAMKQYLSYSIVALIGDVFQASEIMTAQEWKHALLS
                     SRTYDDLKEGLLLVARILEEYREQYSKRQGMEQVLRIRELVAEHLRDKNLSAKFIADE
                     IGLSLGYTRNLFKTIEGVALNEYIGMKRIDEAKDLLSTTKQSINSIRESLGFSNTAYF
                     CTYFKKQTGKSPSEYRNEQRKAAQS"
     assembly_gap    9372..9471
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9472..10734
                     /locus_tag="PLF67_06585"
     CDS             <9472..10734
                     /locus_tag="PLF67_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89714.1"
                     /translation="ACLASPVFAAGASETKAAPAADGPVVISYYTWDDSSHKALIDTF
                     NATHKDIQVKAQILPAADYETKLTTLLSGRADIDAFMEKRQVDMFGQYDNGYLEPLDS
                     YIAATGKPNPAVEAFKSSSVINGDTVVIPWRGGAYYTYYNKKVFEKAGVPTPDYYVKR
                     GEWTWKKFEEVAKAITAADPSLVGSTIYIWGGQGFFQAGQAGDSIVTPDGKIDNIDNV
                     VKQLEMRKRLEDVGAMWSLVDMKVTKLHYSKQFYDGNVGMLLIGEWFPGQMVTGRNDG
                     LLAYDFEDWGITRLPCDQSEYVTVGLPTGNCITSYSKKKQAAFEFINWMSGPEGAAIA
                     ASYGVLPAAPGEAAKAEIAKNLPKGNSLDYFLEEKGNNTANFSPYGTRVETEFAKLQE
                     DFLLGNINIEQFKTNFTKLLNDIIKTTY"
     gene            10807..11706
                     /locus_tag="PLF67_06590"
     CDS             10807..11706
                     /locus_tag="PLF67_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE89715.1"
                     /translation="MNISHSLKRTGTILSFLLPSFIGFIIFVLLPMIMAFGLSFTNYS
                     GGPNFRFIGMKNYVVAFTNPEFLKYLWVTFKFMTVTVVFQIGLGLVFALILARDFRGC
                     SFFRGALYLPNILSSVAVGLAFMFIFEPTSGFMNQFLISVGLPTSKWLASSKSALSVI
                     ITIAIWQNFGYYMVLFIGGLQNISMTLYEAAGIDGANKWKQFVHVTLPGLSPMLFYGI
                     TIAVIRGFQVFDYIYIMTGGQQGGGPAGSTNVLAFDIYLNAFTYFRFGYASAESVVLM
                     IIILAMTFLQSHGQKKWVSYDIV"
     gene            11693..12523
                     /locus_tag="PLF67_06595"
     CDS             11693..12523
                     /locus_tag="PLF67_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89716.1"
                     /translation="MTSYRRKQRIEKIARYVVLAIVVFITLFPLVIVLSSSFKNESEL
                     FDFPFKFIPAKPITDNFTRLKENFPLYIWNSVKLTFVIVIVQLFTAATGAYVFTKLHW
                     KGRDFIFGLYIASMMIPMQAVMIPQFLIIRSINLYDTHMSLVLLGAFTAFGTFLIKQF
                     FMSIPDTYCEAARIDGAKEWTIFSTLMLPMSKAVLVTQIIFSFRYFWNDFFTPLIYIT
                     TPQLKTLPLGMTDFVREQYVYWGPQMAAALISIVPVLLIFLFGQKYFVQGVASSGIKG
                     "
     gene            12537..14312
                     /locus_tag="PLF67_06600"
     CDS             12537..14312
                     /locus_tag="PLF67_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE89717.1"
                     /translation="MKKTQIENFNQDIHVVDYRTPFETFRKQADSLVAVEGRETESLN
                     GKYHFSPDPYESCLRARWFEEKTVSEDGRMLPFDADFDVWPRIPVPSCWNMVRDELYY
                     YENMGLYFRTFRYKPVNNHERAFIHFEGSAYRTYVFLNGTCVGMHDGASTPFSVEVTD
                     HLLPENRLIIAVDAARSKDRVPMDNTDWFNYGGIYRDILFIRTPETFIRNFQTQLKPG
                     KTNRVFAVVELDKEVDAVATYRIRELEIEHEIAIRDGKGQIEFDADLKLWSPEHPVRY
                     EVEITCGEDRVKDLIGFRDIQVDGYKILLNGKRIFLKGICIHEDHITLGKSTTEEVIR
                     ASIADVKAMNGNFIRLAHYPHSRLFAKIADEMGILLWEEIPVYWSINFTNPETYADAE
                     NQLSELILRDRNRASVIIWSVGNENPDSDERLAFMSALAKKTRELDPTRLVSAACLVN
                     TTKLKLEDRLMEHLDIIGNNEYYGWYEPNFDDLLTILNNTDLKKPVIITEFGGGARAG
                     NRGTAETMWTEEFQEAIYRKQYAALDKAVYVQGITPWILYDFRAVRRMNRYQEGFNRK
                     GLIDSDRTTKKLAYYVTKEYYKTKG"
     gene            14316..15464
                     /locus_tag="PLF67_06605"
     CDS             14316..15464
                     /locus_tag="PLF67_06605"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOE89718.1"
                     /translation="MKQPVGLTEEELRDAFAYAIEKTRANIPVFSDGWYPAPASVGNV
                     YPRIPNNEWTSGFWNGMLWLAYLQTKNEAFRTAAENILADYRKRLDERIATNTHDLGF
                     LYILSAKAQYLITGNEEAKDTVLLAADLLMERYHPKAGIIQAWGDLDDPAQRGRIIID
                     CLMNLPLLFWATEITGDQLYRLAAFSHLDRTRETIIREDDTTFHTFYFDTKSGKPLRG
                     TTHQGYSDGSCWARGQAWGIYGLALAYGYTGDPGCLTDAKRLANYFLDHLPEDRVCYW
                     DLIFTQGSEERDSSAAAIAVQGLHLLASFLGGEDSDRQRYAHQADLILSSLVRGYTTR
                     TVPESNGILLHAVYHKPNGIGVNECTVWGDYFYMEALGMKLHSAMTFW"
     gene            15486..>17258
                     /locus_tag="PLF67_06610"
     CDS             15486..>17258
                     /locus_tag="PLF67_06610"
                     /inference="COORDINATES: protein motif:HMM:NF021534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HOE89719.1"
                     /translation="MVPYGHEIRKIVIKDRTSLAATLLELLAPCRGVLVKGGSGLFVG
                     NESAHYSQQVALLEGWSRLLWGIVPLRSGGFSWDGQEDHMAGLIAGTDPDGPYYWGDV
                     GDYDQRMVEMAAIALALLLTPQYYWDPLTERERENLCTWLGMINLHAMSRNNWLFFRV
                     LVNLAFEQLGRKEFAPELMEEDLLELESMYAGDGWYRDQYPFDNYNPLAMQYYALIYS
                     VFRREKDPERSARFVERSRLFAQQHIHFLTEEGPFVPYGRSLTYRFAVISFYSACAFA
                     GLEVLPWGVLKGIVLRNLRWWFQQPIFDRDGFLTVGYGYPSRAMAELYNAPGSPYWSL
                     KSYLVLALEDDHPFWRAEEEPLPQLAKTVLLRQPGVIMQRIDDGDVVLLNAGQYPKFH
                     MAHIAEKYAKFAYSAHYAFSTSVSYYDFEKCGCDSMLYVSDDDQNWRPRREMEVVSVT
                     ERYLRSIWSPFNDVAITTTLVPAGTFHVRIHEIDTKRKLYTKEGGFAIEKYRGWEIPV
                     PIEERYQGEHSLSIVLPWDVSHISDPLLKRKASLVSPAPNLNLAFSTTVVPVLSLVIE
                     AGDSVILIACVGAWRNDDADKPVPS"
BASE COUNT         3782 a         4562 c         4801 g         3913 t
ORIGIN      
        1 gttggagaaa tggagcgccc ggtggggttc gccgtccaag gttacgatcg tgccaccggg
       61 gagcagttgg aggttgtcga ggatcgtatc ttcaattcgc tcggcgctgc cgatcgttcc
      121 aaactctccc tcgatggagt cgtagttttg aagaatgatc gtgagggtgt tcccgtcgct
      181 gctgatcacc gattcaagat cctgcttgcc ttgcttgctc aacaggagca ccatgcgtga
      241 aagaaggttc agcaatcctt tcgaagaggg aattgagcgt tcattggagt acacttcatt
      301 ggcgaacgcg acgtaggaag cgaaggaaag gctttgacgg atatcgttgt tttctttctt
      361 gaccttttct tcaaacgcat agacctgcgc aagattcccg ggttccagga aaaagctggt
      421 cgccccgctc ggcgcatcga tggtgatata gtagggggtg tttccactca tcgtgcgggc
      481 gaagtgacgg gagctgatcg tgaagggatc ccgtttgggc agataggaca tgtagttggt
      541 gttcaccgag attttatcac gggtgagacc aaatccggct atgattataa accacagcac
      601 cacaaaaacc atccagtagc gtcccaccaa aacgtttatg ccgttgacga tcgaagcgag
      661 gtagcctctt cggtacgttt tgatatggtg gaccttgggt ttgcggacaa ggctcagaac
      721 ggccggaagg taggtcgcgg caaggatcgc gcagtaggtg attccgattg caacagagat
      781 tccgaactcc ttcaacgctc ccgtcttgga ggtaagcagg cttaaaaacc ctatgacggt
      841 ggtcaggcag gcaaagagaa tcgttcgaag gatcttccgg gtcgaagcaa tcactccccg
      901 accgatctca tgtccggcaa aatactcgct gattacgtga attgcgtagg aggatccaag
      961 gttgagaacc atcgccggag tgatgatatt gacgatcgtc agactgtagc cgagcagcct
     1021 catcgttcca aaggtccaaa tgatccccat cgccgacatc gagaagggca ggaggacact
     1081 gcgtttggct cggaatgaga ggtagtacac gacaaggatt gccaagagac acagggtgag
     1141 gatgatagcc aaatctcggg agaggtaccg catcagaaca ttggtcagaa tcgctgctcc
     1201 gttgatcgaa acgctcattc cctgtttgac gagcggttca aggatttctt gcaactccgc
     1261 ttccatctgc ggggtgaggt caaccgattc aaagtttatg aacaagccgt gcaaatcctc
     1321 gctgacgagg tagtttttta ccatcggatc gctttgcacc cgttccttga gcagggccgc
     1381 ctcttcatcg gtccatgacg aggatgcaaa aggaacgctc attagccggg ttccccgctt
     1441 ctcaagggta acaaaatcaa agaggctgaa ccgggaggca agataggggg attcggagag
     1501 agcgtcgagc acttcattga tcgaggtcag cgcctcggcg gtatagaggg tggggctttc
     1561 aatcaggagc atgtacgaag aaggaaactc cgtcggttca tcgagctcct ccacctcatc
     1621 gatcggaggt ttttctatcg gagtacgggt ggcgactcct ccttggtagg tggagttcat
     1681 ttcatccttg gccagcaggg aggtgtagtc cgcatcaagg gtgaggaaga gggcatgata
     1741 gagaaaaaat cccgtgatgg ctacgacaag gacgatcgtg atgaccgcat acttgctgac
     1801 aaggtggagg aaggatcctt tcatgacctg ctcctaacgc cggttcctgt ttttccggta
     1861 ggaacgttga ttacgattgc gttctccttt ccggcgtgca gagaggtttt tcccgtttgg
     1921 attatcgggc tttgcccgag agatgattgc cggcccgctc gttttggcaa atgccgtaag
     1981 gatttgttcg gcgatttgat tcggggcact gatataggaa tggtcatcct gaatcgtaac
     2041 ctcttggata tctcgatatt cagctcctac tctcgataca aggtaatcaa ccaggttgcg
     2101 tttgtccagt ccgtcccgac ggcctcgggc gataaaaaga cgggtatact cgtttctcgg
     2161 cggtttcccg agttcctcga tcgtccggta gcgcgttgga tccaaaattn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ctcggcggtt tcccgagttc ctcgatcgtc
     2341 cggtagcgcg ttggatccaa aatttccttg taaagattcg ccaacaccga ggcgagcacg
     2401 gtttcaggca gcttgcttcc cgcaacctca agggcaaggt tgaggaagga gtcattcggt
     2461 tcttccagat ttttcagccg ttcgacgatc tgtttgcgct ttgtcgcgat gatcgcctcg
     2521 atcggcggga tcgcggttcg ctggatgtcg ctttggatgg tgcggcgaat gaattgaaga
     2581 cgcttgaact cccgttgggc gacgaacgtg atcgccacgc cctgttttcc cgccctcccg
     2641 gtacgaccga tccggtggac gtacgcctcg ggatcttcgg gaaggttgta attgatgacg
     2701 tgggtgaggg cgctgatgtc gattccccgg gcggcaacgt ccgttgccac gataatcgcc
     2761 agctgtcggg tccgcatccg attcaagatt gattcacgct gcttttgtga aagatcgccg
     2821 tgaagcgctt cggcatgata gccgcgggcg ataagttttt gaccgacttc gtcacattgg
     2881 agcttcgttc gacaaaagac gattccgtag aatttctctt caatgtcgat gaagcgggtg
     2941 agcgcctcaa atttatcgct ctcgcgaact tcgacgaaga gttgccgggt caagtcggtg
     3001 gtcaacgtct cgtcggcgat gcgaaccacc tgcggcttgt ccatatagcg ttcggccagg
     3061 cggcggatcg gatcgggcat cgtggcgctg aagcagaggg ttcgcttctc tttcggtgtc
     3121 cggaccatga tcgcttcgat ctcctcgaga aaacccatat ccagcatctc atccgcttcg
     3181 tcgaggacga ggaacttgag ttggtccagc acgagcgagc cgcgcctcag atgatcgaga
     3241 atccgcccgg gagttcccac gacgacatga acgcctttct tcaactggcg taattgattg
     3301 acgatcgccg cacctccgta gaccgcagca atctcgaggt tccgcccacc tttgagcgaa
     3361 agaatttctt cagcgctttg aagggcaagt tcccgggtcg gggcaagaat gagagcttga
     3421 accgcattca gttgctcttc aacaagctca aggatcggaa ggccgaacgc agccgttttt
     3481 ccggttcccg tctgcgcttg gccgatgaca tccactcggt ccttcaaaag aagagggatg
     3541 catgttgctt gaatgccggt agggtggata aatcctttgt tttcaatcgc ctgtattgtt
     3601 tgagcggaca ggccgaggtc agcgaacgtt ggtgtgttgg tcatgaagct cccgtgtgtt
     3661 ttggcaccgg gaagagtgta tcggaagagg atgacacttg caagacggct accaccgatt
     3721 ggcgacctct tcggcaaatc cggcgatctt gtccaccacg atggtgtttc cgttgtccaa
     3781 tgtgcatctg ccgctgaaat gaccgaagat ctggtgttgc tcgctggcga tcaaaagcag
     3841 attgaagtgt gaagagcggt cggtgaccgg ttggaagacg agatcgagcc gtccctcgtc
     3901 atccctaatc gtccactctt ccagataaga gccatattcg aacgtcacct cacgaagctt
     3961 gtgcaccact gaatcgtaga tgatcacatt ctcgctcgcc gggcttcgat cggtgaaccc
     4021 gtagccgaga ttcagtccga aggggacttg ttcgacagtg ccgctgcagc tcgcccaata
     4081 ccaggtgttc ctgtacgtcc atttgcctct tccccaatcc aatactccga acgtcgagcc
     4141 cagcggaagc cgttgcaggg cggttccccg acgaatcgtt cccgtcgccc cgaggcagtt
     4201 cactttctca ttgtgataga agcaggtgcg gttcttcttc cagcttgtgg cgatcgtcat
     4261 ggattcaagg gaggagggct gcttcagggt gatgtcgaca tcaagaccga tgatgccgtc
     4321 gcccaaatcc agagaggggc acgcatacat gatgtgccgc tcctctcccc gtttgacgag
     4381 ggcgatgcga atgtgcttgt ttgcccatgc gagctcgtgg tcttccgttg acgtagccgg
     4441 tatgcccaac ttcccaagcg tgagcggggc gatcgcatcg gtttgactga ctcgaccggt
     4501 tttgaggtcg atgtaggcga tcgcaaacac tccggcgtat ccgagatcgc tgaacgttac
     4561 ggcagtagcc cattgttcat cctgattgac gattgcatag tattcccatt ctttgattcg
     4621 gaatgcagtt cccttgacca tcgatcggtc atacttccat accgggcgac gagcccagcc
     4681 ttcttcgatg atcctgccgt tttttcccaa aaggggctga ttgttcgtta cttcgtgctg
     4741 catggctcca tcgtaccata tcaaaaagcg atgagccatg aaaaaccggc ccgaaggccg
     4801 gccggtatcc gcttattgac ccaagacgac tcgcaggaac gtcgtcagga agttgtcctt
     4861 gtgaaggtcg gaagcataga gcgtctgggc gaggttttcc tggctctgac ttccggcaag
     4921 gtagtcgtag aacatcatcg tgtaactgcc catcccctcg tcggttgaat cttcaccgac
     4981 ttcaatcagg agatacgccc ctccggtttt catcggctcg aggagggtgt gttcggcgga
     5041 ggtgaagagc tccgtttgta cttcggctgt cgcataggag gcaagcatcc cggtaggatc
     5101 ggtataggag aagttgttca ggaaggagga ctttccgacg ttgtatccgg ttggcatgac
     5161 ctcgacaaga tccaggtcgt actcaagcgc cgcctctacc aagccggttt tttttgcata
     5221 ctcggaaacc tcggttacca agttgctgag atagctgtcc atcaactctt cctcgttgct
     5281 gccgatatat gctttgacga ggttgcgtgt attctcatcg gcaacgtccg cctcttcggc
     5341 tgcagtggat acgtggtaga tcgcccatcc gccgccggtt tcaaacggac cgacgattga
     5401 accggaatcg gcacccaata aggcggccgc ttcatcttcg tagcggaagt tcggcttgat
     5461 ggcgaagaag ggaatcgttc cgatatatcc atccgcttgg gcgtacatat cgagactgtg
     5521 ctcgcgggcg gcttcctcaa aagtatagag tccgctttgt aaggaagagg caacctcttc
     5581 agcatcttcc aaagaaccga ggctgatgat cgaaagttca atgttctcaa agagctgctt
     5641 atgctccaac gcgtaggcga tcgcttctga atccggatag agggaggaat cgaagaggag
     5701 ataggagaag tcccgaacct tggaagccat gtttccaaca tattcccgct ccgcctcgga
     5761 agcgaggacg gtgctgacgt ccgtcatcac cattcggtag gggaggttgc ggcggatcga
     5821 ggattcgatt gcgctcttcc gttcggtagt ggctttctgg taagtatcga catcgaacgt
     5881 tccgtctttg tggtacgcac cgctgtcaat gatcgccctg ttgagcacat cggtgttgac
     5941 ttggattccc gcttgcttgg caagctgacc gactgcagtg tagaccaccg ttgaatcata
     6001 ggcgctcttc cagatctgat aaagcgtttg ggtgggatcg ctctgactgc ccttgtactg
     6061 gtcggcatag tgctgtacct gatcgaagaa atagttgttg taggcgtaac tgatatcttc
     6121 cttgccgtag gttccgaaga cgaggttgcc ttgattgcgc tttccgagca ccgctccaag
     6181 agtagggcct atgatgaagg aggctccgat gatgagaaga accgccattc ccagaatcca
     6241 cccaacagtc actcgaggct ttgcttttgc tttgctctct tgttgtttct ttccaccctt
     6301 gaaggaaaga actttttttt gctttacatc gttcttgtcg ttaccggaca tcattgacct
     6361 ctttctcgca tcgtgtttcc gcagaacgga cactgtcaag aaaagtacca tttagtgcat
     6421 gcggtgtcaa tcgaacctct tgcttgacat gaaaagagag aagcagatat attaccccct
     6481 agctggttgc ctcgggcagt agcgcagggg ctagcgcact tggttcggga ccaaggggtc
     6541 ggaggttcaa atcctctctg cccgatttga tggcattcct caccgttgag ggatgtcatt
     6601 tttttcatcg ggatcggtgg agttcttacg catcatgctt ttccttcatc gctcgcccga
     6661 acgctccttt ttcctttgtt ctcagattct cggctgcatg atccttcagg aagcccttgg
     6721 ccatgagccg cttggatgga gggtcgatgc gctgtaagac tacgttatcg gctgtaacgg
     6781 agacaatcct gtacctgtcg ccttcatgta ttccatcatc tcccggtcga tcgggattcc
     6841 ggtggcgtta ccccattttc tgatttccaa ctccgtgttc atatgcaccc attctatacg
     6901 atggtcgact gtcatgcagg aacaggtacg gacggaaacg gatggaagtg gtttggaaat
     6961 ctttcgtgta atcaggattg ggccgccttc ctctgctcgt ttcggtattc cgagggggat
     7021 tttcccgtct gcttcttgaa gtaggtgcag aaatacgcag tgttggagaa tcccagactt
     7081 tcccggatgg aattgatcga ctgcttcgtc gtgctcagga gatctttcgc ctcgtcgatc
     7141 cgcttcatgc cgatgtactc gttcagcgcc accccctcga tcgtcttgaa caggtttctc
     7201 gtatagccca gagaaagacc gatctcgtcg gcgatgaact tcgcgctgag attcttgtcc
     7261 ctaagatgtt ccgccaccag ttccctgatc ctgagaacct gctccatccc ctgtcgcttt
     7321 gaatactgct cgcgatactc ctcgagaatg cgggcgacca gaagaagtcc ttctttcagg
     7381 tcgtcatagg ttcggctgga gaggagggca tgcttccact cctgggcggt catgatttcc
     7441 gaagcttgga acacatcacc gatcagggcg acgatggaat agctcagata ctgtttcatc
     7501 gcactgaaca actcgaaatc gtcctgtcgc ttgaggttgg tgatcaaggt gtcaaccgac
     7561 ttggcgtacg cttccgccga gcgctgggac aacgccttct ccatgtccgt cgtttcggtg
     7621 agaaacaggt tgacttcctt ggtgtcctcc tgctcgatct cgctgatgaa cccgatttcc
     7681 gtcagggagc tggacaggag ccggcgcctc atatgctcat accgctgggg aagatcttcg
     7741 agcggcaccc gatccgatga atagaagatg gagagctgct cgtcgaacag gtcgccgagg
     7801 tcgataagaa gctttgcgaa ccgggagatt actttcaccg gttcatacag cgtcagatac
     7861 acatgctgac cttgatccat tgtcagcaaa aatgagttct ttccattgaa gcgaaggtac
     7921 tgcgaagcga aggaaataaa ccggttcttc tgaagaagct ccgattccgg cttctttgtc
     7981 tgtctgagga acaccgcgat gaagaacgtt tggccctcat agagcggcaa agcctcggcg
     8041 ttgagccctc ctttgagata atcgatgaac cggttctccc gcgtcagctg gttgttgatt
     8101 cgcttggatt cccatacaag catcaatgag gcgatgagcg tcaggccgat gaacacgagg
     8161 accagatttt tccgtaaagt gttcagcgga gcgcgaaggt gggacatggg aaggatgaag
     8221 atcagacgcc agttcatctc gccgatatca taccaacatg caagaaactg ttcgccctcg
     8281 tcgtccttga tgttttgacc cccctttggc ccgtcgaacg atgcgatctg agagagcagg
     8341 ggcagggaag tcgctttgcg accgaactga tcgggatcgg gatggctgat gatgtaggaa
     8401 tcctcatcaa tcatataaaa gttcttcgat gttccctcgt catcggcgag gaggctccgc
     8461 gcaagcgtct cggaaaggtt catgatgatt cccgactgta cgtttccgtc ttctccgtaa
     8521 ctgatgttgc agattgtata gagattggta ggggtgttcg gttttttggg gagagaaagc
     8581 ggaatcgtct tgtcggtgaa caccgatcgc cgctggtaga ggtgtttgtt cttcgggtgg
     8641 gtggccagaa actcaagcag cgtggaatcg ctcaattccc cgggcacctc cgccccgctg
     8701 aggctggaga aaaagccgta ttcactgtta tacagataga tcgaatggat ataggagttc
     8761 cgtgccacgg tgttttccaa acggaggtac gcaccgagca tcgccttgtc cttgggaaga
     8821 ttctgtaata aatagtcacg gatctcctga gcatcgctca ggtcgtccag aatgggaacc
     8881 gtgttgaacc gcatctgccg gtaataggtg ctgatcatct tcagcgaagc gaggaagtcg
     8941 tcgacgatca acgattgagc cacgttggtg aacatcccgc tgaacaacag cgccataatg
     9001 atcatggtaa gcaggaagat ggaagcgatc ggaatgaaaa ccttcagaat ccagatggga
     9061 gtcactgaat ttttccgccc ttttagccta gtcatatcac tcctcctcat ggatatcaat
     9121 acaccatcaa gttgcacaaa tcaatacatt gttcgttttt caaaacccga atcttcactt
     9181 ttcaaaagaa aacggttctt tcaatattgc aatcaaaaca cactcatgca aggctgaatg
     9241 taccgactca catgttcaaa gagaaaggag gaacatcatg agaacaaaga aaatactgct
     9301 tgtcttaatg ctcatagcgt gcttggcaag cccggtattc gccgcagggg cgtccgaaac
     9361 gaaggcggca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgtgcttg
     9481 gcaagcccgg tattcgccgc aggggcgtcc gaaacgaagg cggcacccgc tgctgacggc
     9541 cctgtcgtta tatcttatta tacgtgggat gacagcagcc acaaggcgct tatcgacacc
     9601 ttcaacgcca cgcacaagga tattcaggta aaagcgcaga ttcttcccgc agcggactac
     9661 gaaacgaaat tgaccacgct gctcagcggc cgcgccgata tcgatgcgtt catggaaaag
     9721 cgccaggtag acatgttcgg gcagtacgac aacgggtatc tggaacccct tgattcctac
     9781 atcgcagcga ccggcaagcc caacccggcc gttgaagcgt tcaagtcgag ctccgtcatc
     9841 aacggtgaca cggtggtgat tccatggcgc ggcggtgcgt actacaccta ctacaacaag
     9901 aaggtcttcg agaaggccgg agttcccacg ccggattact acgtgaagcg cggcgaatgg
     9961 acctggaaaa agttcgagga agtcgccaag gcgatcaccg ccgccgaccc gagcctggtc
    10021 ggttccacga tctacatctg gggcggacag ggcttcttcc aggccggaca ggccggggac
    10081 tcgatcgtca cgccggacgg caagatcgac aacatcgaca acgtcgtcaa gcagctggag
    10141 atgagaaagc ggctggaaga tgtcggggcg atgtggagcc tcgtggacat gaaagtcacg
    10201 aaactgcact attccaagca gttctatgat ggaaacgtgg gaatgctcct gatcggcgaa
    10261 tggttccccg ggcagatggt aaccggacgg aacgacggat tgctcgccta tgactttgag
    10321 gactggggca tcaccagact cccgtgcgac cagagcgaat atgtaacggt cggacttccc
    10381 accggaaact gcatcacttc atactcgaag aagaagcagg ccgcgttcga gttcatcaac
    10441 tggatgagcg gacccgaggg cgcggcgatc gcggcgtcgt acggagttct tcccgcagcg
    10501 ccgggcgaag ccgccaaggc cgaaatcgcc aagaatcttc cgaaggggaa ttccctcgac
    10561 tacttcctcg aggagaaggg gaacaatacc gccaacttca gtccttatgg aaccagagtc
    10621 gaaacggaat tcgcgaagct tcaggaggac ttccttctgg gcaacatcaa catcgaacag
    10681 ttcaagacga acttcacgaa gctcttgaac gacatcatca agacgaccta ctgatcgtcg
    10741 cgacgaggcc tatgacgctc tcttccatgg aacttccgtg gaagagagcc ctcttggagg
    10801 aactgcgtga acatttcaca ttctttgaag agaaccggaa ccattctttc gttcctgctc
    10861 cccagcttca ttggattcat catcttcgtc ctgctgccga tgatcatggc gttcggactt
    10921 tcgttcacca actattcagg cggcccgaac ttccggttca tcgggatgaa gaactatgtc
    10981 gtggccttca cgaatccgga gtttttgaag tacctctggg tcaccttcaa atttatgacc
    11041 gtcacggtcg tattccagat cggtctggga ctggtgttcg cactgatcct tgccagggac
    11101 ttccgcggct gctcgttttt ccgaggagcc ctgtatctgc cgaacatcct ctcctccgtc
    11161 gccgtcggcc tcgcattcat gttcattttc gaacccacca gcgggttcat gaaccagttt
    11221 ctcatatcgg tgggactgcc gacgtcaaaa tggcttgcca gcagcaagtc ggcgctttca
    11281 gtcatcatca ccatcgcaat ctggcaaaac ttcggatact acatggtcct cttcatcgga
    11341 ggccttcaga acatcagcat gacgctgtac gaagccgcag ggatcgacgg tgcgaacaaa
    11401 tggaagcagt tcgtccatgt cacgctaccc gggctctccc cgatgctctt ctacggaatc
    11461 acgatcgcgg tcatcagggg ctttcaggta ttcgactaca tatacatcat gaccggaggt
    11521 cagcagggag gcggtccggc gggatccacc aacgtccttg cgttcgacat ctacctcaac
    11581 gcgttcacgt acttcaggtt cggctatgca tcggccgagt cggtcgtgtt gatgatcatc
    11641 attttggcga tgacgttcct gcagagccat ggacagaaaa agtgggtgag ctatgacatc
    11701 gtatagaaga aagcagcgaa tcgaaaagat tgcaagatat gtggtgttgg cgatcgtggt
    11761 gttcatcact ctgttcccgc tcgtgatcgt cctctcgtca agcttcaaga acgaaagcga
    11821 gctcttcgat ttcccgttca agttcatccc cgcgaagccg atcacggaca acttcacccg
    11881 gttgaaggag aacttccccc tctatatctg gaactcggtc aagctgacgt tcgtcatcgt
    11941 tatcgtgcag ctgttcaccg cggcgacggg ggcgtacgtg ttcacgaagc tccattggaa
    12001 ggggagggat ttcatcttcg ggctgtacat cgcatcgatg atgattccga tgcaggcggt
    12061 gatgatcccg cagtttttga tcatcaggag catcaacctc tacgatacgc atatgtcctt
    12121 ggtgctgctg ggagcgttca ccgcgttcgg caccttcctc atcaagcagt tcttcatgtc
    12181 gattcccgat acatactgcg aagcggcccg aatcgacgga gccaaggaat ggacgatctt
    12241 ctcgacattg atgcttccga tgagcaaagc ggtactggtc acccagatca tcttctcgtt
    12301 ccggtatttc tggaacgact tcttcacgcc gctcatctac atcaccacgc cgcagctcaa
    12361 gaccttgccg ctggggatga cggatttcgt gcgggagcag tatgtgtact gggggccgca
    12421 gatggcggcg gccctgatct ccatcgtccc cgtgttactc atcttcctct tcggacagaa
    12481 atacttcgtc caaggtgtcg catcgtcggg aatcaaagga tgacaaggaa acagagatga
    12541 aaaagaccca aattgaaaac ttcaatcagg atatccacgt ggtggactat cgcaccccgt
    12601 tcgagacctt ccggaaacag gcggacagcc tggttgccgt cgaagggcga gagaccgaat
    12661 cgctcaacgg caaataccat ttctctccgg atccgtacga aagctgcctg cgggcccgct
    12721 ggttcgagga gaagaccgtc agcgaagacg gccgcatgct tccgttcgat gccgacttcg
    12781 atgtctggcc gagaatcccc gttccctcct gctggaacat ggttcgggac gagctctact
    12841 actatgagaa catggggctc tacttcagaa ccttcaggta caagccggtg aacaaccatg
    12901 aacgggcgtt catccatttc gaaggaagcg cctaccggac ctacgtgttc ctcaacggga
    12961 cgtgcgtcgg gatgcatgac ggggcgtcca ccccgttctc cgtcgaggtg acggaccatc
    13021 tgctgccgga aaaccggctc atcatcgccg tcgatgccgc tcggagcaag gatcgggttc
    13081 ccatggacaa caccgactgg ttcaactacg gcggcatcta tcgggatatc ctctttattc
    13141 gaacccccga gaccttcatc aggaacttcc agacccagtt gaagccgggg aagacgaacc
    13201 gggtgttcgc tgtggtcgag ttggacaagg aggtcgatgc cgttgccacc tatcgcatcc
    13261 gggaactgga gatcgagcat gagatagcga tccgtgacgg aaaggggcag atcgaattcg
    13321 atgccgacct caagctctgg tcgccggagc atcccgtcag atacgaggtg gagatcacct
    13381 gtggggaaga ccgggtgaaa gacctcatcg gcttccggga cattcaagtc gatggataca
    13441 agattctcct caacggcaag cgaatcttcc tcaaagggat ctgcatccat gaagaccaca
    13501 ttaccttggg caaatcgacc accgaggagg taatccgcgc ttcgatcgcc gatgtgaagg
    13561 cgatgaacgg aaacttcatc cgtctggccc actatccgca ctcacggctg tttgcaaaga
    13621 tcgccgacga gatggggatc ctcctctggg aggagattcc cgtgtactgg tcgatcaatt
    13681 tcaccaaccc ggaaacctat gccgatgcgg agaaccagct ttcggaactg atcctcaggg
    13741 atcggaaccg cgcaagcgtc atcatctggt cggtggggaa tgagaacccg gattccgacg
    13801 agcggcttgc cttcatgtcc gcgctggcga agaagacgag ggagctcgac ccgacccgcc
    13861 tcgtcagcgc cgcctgtctg gtcaacacga cgaagctcaa gctggaagac cggctgatgg
    13921 agcatctgga catcatcgga aacaatgaat actacgggtg gtatgagccg aatttcgacg
    13981 atcttttgac gatcctcaac aacaccgatc tgaagaagcc ggtcatcatc acggagttcg
    14041 gcggcggggc gcgggccggc aaccgcggaa ctgcggagac gatgtggacg gaggagttcc
    14101 aagaagcaat ctacaggaag cagtacgccg cgctggacaa ggcggtctat gtccagggaa
    14161 tcaccccgtg gatcctctac gacttcaggg cggtgcggcg gatgaaccgc tatcaggaag
    14221 gattcaaccg caaaggcctg atcgacagcg accgcacgac gaagaagctg gcgtactacg
    14281 tgacgaaaga gtattacaag acgaaaggat gaatcatgaa gcagcctgtt ggactcaccg
    14341 aagaagaact gagagatgcc tttgcatatg cgatcgagaa gacaagagcg aatattcccg
    14401 tcttctccga tgggtggtat ccagccccgg cgagcgtcgg aaacgtctat ccgaggatcc
    14461 cgaacaatga atggaccagc ggcttctgga acggaatgct ctggcttgca taccttcaga
    14521 cgaagaacga agcgttccgc accgcggcgg agaacatact cgccgactat cggaaacggc
    14581 tggacgagcg gatcgccacc aatacccacg atctcggatt cctctacatc ctctcggcga
    14641 aagcccagta cctgatcacc ggaaatgaag aggcgaaaga cacggttctt ctggcggccg
    14701 acctcttgat ggagcggtat catccgaaag cgggcatcat tcaggcgtgg ggggatctgg
    14761 atgaccccgc ccagcgggga cggatcatca tcgactgcct gatgaacctt ccgctcctct
    14821 tctgggcgac ggaaatcacc ggggatcagt tgtatcggtt ggctgcgttc tcccatctcg
    14881 atcggacacg ggagacgatc atccgcgagg atgacacgac gttccatacc ttctacttcg
    14941 acacgaaaag cggcaagccg ctcaggggca cgactcatca ggggtatagc gacggctcct
    15001 gctgggcgcg gggacaggcg tgggggatct acggtcttgc gctcgcctac gggtacaccg
    15061 gggatcccgg ctgtctgacc gacgcgaagc ggctggcgaa ctattttctc gaccatctgc
    15121 cggaagaccg tgtctgctac tgggatctga tcttcaccca aggttcggaa gagcgggaca
    15181 gctcggcagc ggcgatcgcc gtgcagggac tccatctgtt ggcatccttt ttgggcgggg
    15241 aagattccga ccggcagcgg tatgcacatc aggcggatct gatcctctca tcgctggttc
    15301 ggggatacac gacccggacg gtccccgaat ccaacggaat cctcctccat gcggtctacc
    15361 acaagccgaa cgggatcggc gtcaatgaat gcaccgtctg gggcgactac ttctacatgg
    15421 aagcgttggg aatgaagctt cacagcgcga tgacgttctg gtgaacggga aaaggaagca
    15481 cgaccatggt tccatacgga catgagatca ggaagatcgt catcaaagac cggacatctt
    15541 tggcggccac cctgctggag cttctcgctc cgtgcagggg tgttctggtc aagggagggt
    15601 cgggcctctt cgtgggaaac gaatccgccc actactctca gcaggtggcg ttgctggagg
    15661 ggtggtcccg cctgctctgg gggatcgtgc cgcttcgaag cggcgggttc agctgggatg
    15721 gtcaggagga ccatatggcc gggcttattg ccggaacgga tcccgatgga ccgtattatt
    15781 ggggcgatgt gggggattac gaccagcgga tggtcgagat ggcggcgatc gccctggcgt
    15841 tgctgctcac ccctcaatac tactgggatc cgcttaccga acgggagcgc gagaatctct
    15901 gcacctggct cgggatgatc aaccttcatg cgatgagccg gaacaactgg ctgttcttcc
    15961 gggtgctggt gaatctcgct tttgagcagc tcgggcggaa ggagttcgcc cccgagctga
    16021 tggaagagga tctgctcgag ctggaatcga tgtatgccgg agacggctgg tatcgggacc
    16081 aatatccgtt cgacaactac aacccgctgg cgatgcagta ctatgcgctg atctactctg
    16141 ttttcagaag agagaaggat cccgagcgct cagcgcgctt tgtcgagcgc tccaggctct
    16201 ttgcgcagca gcacatccac ttcctcactg aagaaggtcc gtttgttccc tatggacgct
    16261 ccctcacgta tcgcttcgcg gtgatctcct tctacagcgc atgcgccttc gccggtctcg
    16321 aggtgcttcc atggggcgtc ctgaaaggga tcgtcctgag gaacctcaga tggtggttcc
    16381 aacagccgat cttcgaccgg gacggctttt tgaccgtggg gtatggatac ccgtcccggg
    16441 cgatggcgga gctgtacaac gctccgggat cgccgtactg gtcgctcaaa agctatctgg
    16501 ttctcgccct tgaggacgac catccgttct ggcgggcgga ggaggagcct ctgccgcagc
    16561 ttgcgaagac cgtgctgctc aggcagccgg gagtcatcat gcagcggatc gatgacggtg
    16621 atgtcgtcct gctcaacgcg gggcagtatc cgaagttcca tatggctcat atcgccgaga
    16681 aatacgcgaa gttcgcctac agtgctcact atgcgttcag cacgagcgtc tcctactacg
    16741 atttcgagaa atgcggctgc gacagcatgc tctatgtaag cgacgatgac caaaactggc
    16801 gccccagaag ggagatggag gtcgtctccg tcaccgagcg gtatcttcgg tcaatctggt
    16861 ctccgtttaa tgacgtggcg atcaccacga ccctcgtgcc cgccggaacg ttccacgtcc
    16921 ggatccatga gatcgatacg aagcgcaagc tctatacgaa ggagggcggg ttcgccatcg
    16981 agaagtaccg ggggtgggag atccccgttc cgatcgagga acggtaccag ggcgagcatt
    17041 ccctttcgat cgtgctgccg tgggatgtca gtcacatcag cgatccgctc ctgaagcgaa
    17101 aggcatcgct cgtcagcccc gctccgaacc tgaacctcgc gttctcgacc accgtggtac
    17161 cggttctgtc gctggtgatc gaagcgggag attcggtcat cctgatcgct tgcgtcggcg
    17221 cttggagaaa cgacgatgcc gacaaaccgg ttccctcg
//
LOCUS       DAOSLI010000040        15804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3885_length_15804_cov_25.097593, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000040 DAOSLI010000000
VERSION     DAOSLI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 15804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15804
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_15804_cov_25.097593"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..602
                     /locus_tag="PLF67_06615"
     CDS             207..602
                     /locus_tag="PLF67_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOE89720.1"
                     /translation="MKERRQTIQKGLVWDALNEHMNHPSAEQIYESIIQKHPTISRAT
                     VYRNLNSLVEDGIIRRVQVPGGPDRYDRILEQHFHIQCVVCKSIHDLEMTKDLSIGLE
                     DLDLKGYQLESYQVVFNGVCPECQKEKLS"
     gene            623..1165
                     /locus_tag="PLF67_06620"
     CDS             623..1165
                     /locus_tag="PLF67_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOE89721.1"
                     /translation="MDLKGTKTEKNLWEAFAGESMARSKYTYYASRAKKDGYVQIGKL
                     FEETAFNEQEHAKVWFKLLQEGGEIQGTAQNLKDAAAGEHYEWTEMYVNFAKDAREEG
                     FTKIASLMDMVAKIEKHHEARYLNLLKNIEEEAVFAREEEQAWICSNCGHIAFGKKAP
                     ALCPVCAHPQAYFEIEAKNY"
     gene            complement(1212..2456)
                     /locus_tag="PLF67_06625"
     CDS             complement(1212..2456)
                     /locus_tag="PLF67_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE89722.1"
                     /translation="MKYFFDPHLHVMTLEHPNLLSFFGSLESGIPDLITSGALSPSYI
                     VGGGKRSKGSSLLNRITNTLTTFEQPIGATFIMMEDDLKGAFESREEKAPRPEKPYIS
                     DGKLHIRSLVYDKIGLCPLLMDFSTSEIGKDSLYYPTEKSVRIIDYIADTVEGIKAYQ
                     EARPDGLFEFFPFLGINPPVHDIGFIRELLERFIVIEKRPEDQRRFWGIKLYPPLGYN
                     PWPDDPTERDKVELIYEFCSKHKIPIVTHCDDQGFRGISAKEAWKYTAPFAYKPALER
                     YPTLTIDFAHYGWQYNQLQKGPLAVISSLATRTPDSPWFYELTEMMHLYPNIYSDVSF
                     SGCDPAFYVQLSNYINNLERDEERETVLSRTLFGTDFSVHLIKVESYTSYYRIFEESP
                     FSDEEIDRFVSVNPMRYLGLCD"
     gene            complement(2475..4157)
                     /locus_tag="PLF67_06630"
     CDS             complement(2475..4157)
                     /locus_tag="PLF67_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOE89723.1"
                     /translation="MRSEPQNFLERIIEDDLANGRVPHNEIVTRFPPEPNGYLHIGHI
                     KSIALNFGLAEKYGGRCHLRLDDTNPEKEEMEYTEAIMRDIRWLGFDWGEHEYHASDY
                     YRQLYEIALHLIETGQAFVCELDPDQMREYRGTLTEPGKNSPYRDRPIEENLRLFKEM
                     KEGKHPEGSYVLRAKIDMASPNMNLRDPVLYRIKFATHPRTGDDWCIYPMYDYTHPIS
                     DAIEGITHSICTLEFEDHRPAYDWATSIDLIDHTPRQYEFARLNIDYTLMSKRRLLAL
                     VQMGVVDGWDDPRMPTISGIRRRGYSPEAMLDFVGRIGVAKVEGIVEHSLLEFCVRED
                     LNKRAKRLMVVLDPIKVVIDNFPEGKVEYVEALNNPEDPEGGTRLVPFSREVFIERED
                     FMEKPPKGYHRLSIGTEIRLIYAYYITAHSVVKDDDGNVLEVHCTYDPESRGGQTADG
                     RRVRGTSHWVSAAEGVSIEVRLYDKLFLAEEPGKETGNFLDDLNPESLIVVEDAKAEP
                     EVLKAKVGDYLQFIRNGYFCVDSKDSTPEKPIYNRTVTLRDTWARMKKKMGI"
     gene            complement(4244..4762)
                     /locus_tag="PLF67_06635"
     CDS             complement(4244..4762)
                     /locus_tag="PLF67_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89724.1"
                     /translation="MKRIQLKEFTFLVPPARVFRDWRSLVLTTHPVAELPNAPMLCAV
                     SGEPFTFEMRLSIPPGGYVGLCAYHIDTVFASVGVNADSIRTFSMIRGYPSKVIYPFS
                     SDKDTLKWRMQWDRKRTMIGFQREEDDPVAWVGVFELPGTSASISFGPFFVSDGEAYQ
                     ATGEDLRFTASE"
     gene            4855..5826
                     /locus_tag="PLF67_06640"
     CDS             4855..5826
                     /locus_tag="PLF67_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOE89725.1"
                     /translation="MIAVIGEALIDFIGHGTGSRTVPFESHIGGCGINTAVAAAKAGS
                     AVGFFCKISSDLFGKRHLEHLVGNKVMFDPTLCNAKEPSLLAFASLDAVGKASYAFYW
                     KNSAPVSMSVEELLKTMSDHTDLRVVHIGSLALTLEPIGEVILEALERYEPKPIIFLD
                     PNVRPSVIDDWNQYRTHIEKAIDLASFVKLSDEDLQLIHPETEVHAKAAELARTKKKH
                     IILTLGREGAVWYGPEGKEVSHPIIDLPLADTVGAGDTFSGTILSFLHDRGYFGKEGE
                     PPQLLEIPIKVRQEALKWASAASAINCSRPGCDPPSRKELLALLETL"
     gene            5849..7147
                     /locus_tag="PLF67_06645"
     CDS             5849..7147
                     /locus_tag="PLF67_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOE89726.1"
                     /translation="MSKDQRKMKTLLRLIRFGIILSLSLFIALSLYSWTKSIGRDKQR
                     KELAVLLKQTVEQEGVEAILSLSGVAVENIFHGEEGLILFEGSDTPWRYSADELQTIS
                     VYEKVNKSVVNITTNTNRSASDFLDVLPGHGTGSGIVLSSDGYILTNAHVVEGAETIM
                     VGLYNNQTYQATLVGVDSEDDLAVVKIDVGKDLMLYPIALGTSSELRVGQKVIAIGNP
                     FGYDRTMTIGVVSGLNRPVRTGEGRVVMNAIQTDASINPGNSGGPLLNSRGEVIGINS
                     SIFSTSGSSQGINFAIPIDTAVEVVGELIRTGKVSRGWIDASMLQLTPRLVEYAKLPI
                     SQGVLISQLAPGGNAEKAGLKGGTRQVQYGSSIFYLGGDIIVAIDNEKVEDLNGLYLA
                     LLPKKSGEKVAVTVNRSGEVKKIDVVLIERTMSHIRALVR"
     gene            7149..9218
                     /gene="uvrB"
                     /locus_tag="PLF67_06650"
     CDS             7149..9218
                     /gene="uvrB"
                     /locus_tag="PLF67_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOE89727.1"
                     /translation="MDDRQVVDGSWGGETITNVHGEVSYQERRFQVISEFEPAGDQGQ
                     AIDKLSEGVLEGQRYQTLKGVTGSGKTYTMAKIIERVQRPTLVLSHNKTLAAQLYREF
                     KSFFPDNAVEYFVSTYDYYQPEAYVPGKDLYIEKDADINAEIERLRLSASFSLMERRD
                     VIVVATVSCIFGLVNPVSIRDMVYTFKRGERFNHKEILNQLVRMQYERNDAILKRGSF
                     RVRGDVIEICPSYLENAVRISVDWDEIAEILWFDPISGEKQGSVETFSLYPAKQFVMP
                     PEQVKAAIDRIRLEMEEQVEYFESLGKPLEAERIRTRVEYDLEMLSEIGYCSGIENYS
                     RQLSNRKAGERPAVLLDYFPPDFLTFIDESHVTLPQVGAMYEGDRSRKLNLVTYGFRL
                     PSALDNRPLKAEEFEQVIGQRVFVSATPGKIELEKATNVVEQLIRPTGLLDPKVEVRP
                     TEGQMEDLYGEIRATVAKNQRVLITTLTKKMSEDLTDYLSGLSLKVRYLHSEIETIER
                     VELLRDLRLGVFDVLVGINLLREGLDLPEVSLIAILDADKIGFLRSATSLVQTIGRAA
                     RNADGRVIMYADRHSDAMIEAIRETERRRKAQLAYNAEHGITPKTIIKAISDIIEREK
                     ETTQAIQSREIEILKGGYNLLLASGRKKYVKALETEMFEAAKDLEFERAALLRDEIQR
                     IERGEIE"
     gene            complement(9188..10084)
                     /locus_tag="PLF67_06655"
     CDS             complement(9188..10084)
                     /locus_tag="PLF67_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOE89728.1"
                     /translation="MKPFLWLFLAVIVLASCELIPFESARNIHHIGIGISYRGTDLLV
                     LEGPINDAVALKKSFHSFFSAEPFESTLLLQQGDEKGAYTLDEKNLPTKDLVIETIIR
                     TLGGMKKNDLLIITYSGHAVEDGSLVLAPDNSNGLLLDDNLDGALLGVDELISLFDRS
                     EATILLILDSCYAGSFIPESGDVSTIDSKNRFENLRDGYRQGIERNPRLFVLAATTAD
                     NTSKEPRFGSPIHGYFTKALLEALDTTSKQVTLDQVYHHIHEHQGFPTEGSNKHLYQH
                     PIVSGGALDLVLRYSISPRSIR"
     gene            complement(10117..11097)
                     /locus_tag="PLF67_06660"
     CDS             complement(10117..11097)
                     /locus_tag="PLF67_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOE89729.1"
                     /translation="MKTRTILFLLLPILLLVSCELVQDQPPQGKVYGVFIGLDYRNTT
                     FTNPADEYGRLLPLKGTLNDAREMKTAFGHIAGLAKLEMDSYLMYQEGDTKDQSTYEM
                     ITVGGTPIRSYASKANLALLLDALTDAIKEEDLLILTYHGHGGEDVLFMAPESNDDYD
                     IELKVTEKLLKELQPIKGRKLVILDSCESGMSVPASESSVSLVRSPSLSDWFDTYFSD
                     KPYAIPPVTLFTATAHTDSYEMGDDHIHGVFTLALLEALGGSHDEDDGFLMGTEIPAK
                     HNGVISVDSLYRYIKQHQKLPLRTTIYRPASMYQHPMTNGGAMDFILFRL"
     gene            complement(11094..11645)
                     /locus_tag="PLF67_06665"
     CDS             complement(11094..11645)
                     /locus_tag="PLF67_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89730.1"
                     /translation="MKRPLVLSLLLLFALPIAGGNFVTTISYVPSAYLSIPHFEDELP
                     FRTSMSNTFIFEPFGYEADTWSVNASLKGAFVTPSLPWGSYRARGFTSLGLGARFSYS
                     LTDRFALFGSGGIAVNRYTAIEWAFLSFWAEAGLQYTILETTNTELSAILPIMIDLRK
                     EITALSIGLGLRWSFDPYGGGLL"
     gene            complement(11706..12740)
                     /locus_tag="PLF67_06670"
     CDS             complement(11706..12740)
                     /locus_tag="PLF67_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89731.1"
                     /translation="MILQCSLQATYPDFTLDLAFTVQEGDLVCIIGPSGCGKSTTLSM
                     IAGLIHAETGSIILGGEDISQKEVHKRKIAMVFQDYSLFPHMNVEQNIAYPLKNLKLK
                     KRQRVQAVKELLELVNLQGYEKRKSTRLSGGERQRVALARALASEPKILLLDEPLSAL
                     DAKLRTRLREEIRRIHEETGITTIYVTHDQDEALAIADQVIVMDQGRIIQQGTPEEIY
                     RTPRTKFVATFTGEGTILPASAFARTLRNPYQLEIPSKHRNPTNTSLFFRPHASHIVE
                     DPGEVRLQYLPFLAFDEVRVIRSEFQGTHWRNSCLWNEHELLCHSDRKPKKEIISLHV
                     DITDLSYFEE"
     gene            complement(12737..14404)
                     /locus_tag="PLF67_06675"
     CDS             complement(12737..14404)
                     /locus_tag="PLF67_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE89732.1"
                     /translation="MSDYRRFYRQKSIGPLVILFSLFALPLALTIQSGVWEEGSFTLK
                     RIVSAILDPYTQRILLFTLKQAFYSTGAALVIGLPGAYILATYTFRGKKLIKALATIP
                     FVLPSILVVLGFVMFFGNSGTLNMVLMKVFDLDDPPLKLLYSLGAIILAHTFYNFPII
                     MSIVATAWEGLDTKLEAAAATLGSAPRKVFRTITLPRLVPSIISAAALVFLFCFNSFA
                     IILVLGGGPQFTTLEVEIYRQARMMGDPSSASALAIVSMLINGILFLGYAFSQRTLTV
                     TETVAERAFEERKPSPMRTFFILLYSLFTIIFLLGPIISVLYRSFSKGASRASETTFS
                     TTWYRQLLGFEQSSSHMGSALTALINSTLIGVAVSILTVTLALLLSVSIARRKKSSSF
                     LELFGMLPMMTSSVIVGLGYVLIARLLRPSLFGTYLLITLAHTVIAFPFALRTILPNV
                     RSLSETYLHASYTLGSSPQKTFRTVEIPLLRRTLLTAAVFAFALSLGEFNATLILSSS
                     RIVTLPVVMYRLIGSYHFQGACALGSILIITSGLTFFLGEVAKGDSV"
     gene            complement(14401..15426)
                     /locus_tag="PLF67_06680"
     CDS             complement(14401..15426)
                     /locus_tag="PLF67_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE89733.1"
                     /translation="MKMHRFVLGVCITLTIAFPLFSMGAQKEQQLTVYAYDSFSGDWG
                     PGKEVVAAFEAKHDIPVKVISAGDGVEMLTKVIAEQDHPYADVVIGISDEMATRAYRA
                     DLFEPYHSPLLHEIPEFLHFDSEYRLLPFDYGNFAFIIDSERIPKEDWPTSLEDLTHP
                     RFRKNIILIDPRTSNVGLGLLLWTIHIFGEEGWLDWWKAMKDQVLTITDGWSGAYGLF
                     IEGEAPIVLSYTTSPVYHYTYEGSTRYQALIFDEGHQATIEGVGMLRSSKRKEEAKLF
                     IDFLLSEAQLPIALANSMYPTNMNTALTEGFAYAPKPGVSLRMESEVIEKKLDRWLHE
                     WTEVMSR"
     regulatory      complement(15463..15554)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         3745 a         4023 c         4537 g         3499 t
ORIGIN      
        1 atctcctcca acattttctg atgttagtga ctatctaaca tataaacaaa aagtcacgga
       61 gtttattcat gcaaggaatc gaagaatcac cgaatatgca tccgtaacat ctgtccggca
      121 accatgtttt tcctgaactt tgttgacacc cccatgggca tcatttatga tgttaatgaa
      181 attccgtatc agtagcatat caatctatga aagaaagaag acagacaata cagaagggct
      241 tggtgtggga tgcgctcaat gaacatatga accatccaag cgccgaacag atctacgaat
      301 cgattattca gaagcatccg acaatcagca gggctaccgt atatcgcaac ctcaactccc
      361 tcgtggaaga tggaataatc cggcgggtgc aggttccggg aggaccggat cggtatgatc
      421 ggattcttga gcagcacttc catatccaat gtgttgtgtg caaaagcatc catgatcttg
      481 agatgacgaa ggatttatcc attggacttg aggatctcga tttgaagggg tatcagttgg
      541 agtcctacca agtagtgttt aacggggttt gccccgagtg tcaaaaggaa aaactgtcgt
      601 aaggcaggac aaggagtgtc acatggattt aaagggaaca aagaccgaga agaacctgtg
      661 ggaagcattc gcaggggagt cgatggctcg aagcaagtat acctattacg cctcgcgggc
      721 taaaaaagat gggtatgtac aaatcggcaa actgttcgaa gaaaccgcat tcaacgaaca
      781 ggagcacgcc aaagtctggt tcaagcttct gcaggaaggc ggcgagattc agggaaccgc
      841 ccagaacctc aaagatgctg cagccggcga gcactacgag tggacggaga tgtatgtaaa
      901 ctttgccaag gacgctcggg aagaaggctt caccaagatc gcttcattga tggatatggt
      961 cgccaagatc gagaagcacc atgaagcccg ttaccttaac ttgctgaaga acatcgaaga
     1021 agaagctgtc tttgcccgtg aagaggagca ggcttggatc tgctcgaact gtggacacat
     1081 cgcatttggc aagaaggctc ccgccctttg tcccgtttgc gcccaccctc aggcatactt
     1141 cgaaatcgag gcgaagaact attaattttt tggatcgaga gaaggaaggc aggtcttcgg
     1201 atctgcctct tttagtcaca gaggccaaga tatctcatcg gattgactga aacgaagcga
     1261 tcgatctctt catcgctgaa gggggactct tcaaagatcc gatagtacga ggtatatgat
     1321 tcgaccttta tcagatgtac cgagaaatcg gtgccgaaca acgtccggga aagaacggtt
     1381 tcccgctctt cgtcccgttc gaggttgttg atgtagtttg aaagctgaac gtagaaggcc
     1441 ggatcgcaac cgctgaagga aacatcgctg tagatgttcg ggtagagatg catcatttcc
     1501 gtcaattcat agaaccaggg ggagtccgga gtcctagtcg ccaaactgct gatcaccgca
     1561 agcggaccct tctgcagctg gttgtactgc catccatagt gggcgaagtc gatcgtcagg
     1621 gtgggatagc gctcaagggc cggcttgtag gcaaacggag ccgtatattt ccatgcttcc
     1681 ttggcactga ttccccgaaa gccctggtcg tcacagtggg ttacgatcgg gatcttgtgt
     1741 ttcgagcaga actcatagat caattcaact ttatcccgtt ctgttggatc atccggccaa
     1801 gggttgtacc ccaaaggagg gtagagtttg atgccccaaa accgtctttg gtcttcaggt
     1861 cgtttttcga tgacgataaa gcgctccaac agctccctga tgaagccgat gtcatgaacc
     1921 ggcggattga ttcccaagaa gggaaagaac tcgaagaggc cgtcggggcg tgcttcctga
     1981 taggctttga ttccttcgac cgtatcggcg atgtaatcga tgattcgtac gcttttttcg
     2041 gttggataat agagggagtc tttaccgatc tcgctcgtcg aaaaatccat caacagcgga
     2101 caaaggccta ttttgtcata gacaaggctc cggatgtgga gcttgccgtc gctgatatag
     2161 ggtttctccg gacgtggggc tttttcctcc cggctttcaa aggcgccttt aagatcatct
     2221 tccatcatga tgaacgttgc accgatcggc tgctcgaacg tggtgagggt gttggtaatc
     2281 cggttgagca gcgatgaacc tttggagcgc ttgcccccgc cgacgatgta ggaaggtgaa
     2341 agcgcaccgc tggtgatgag atcgggaatg cccgattcaa gagagccaaa gaaagagagg
     2401 aggttgggat gctccaacgt catcacatgg aggtgaggat caaagaaata cttcatcttc
     2461 ctccttcttc cgggtcagat ccccatcttc ttcttcattc gggcccacgt atcgcgcaag
     2521 gtaaccgtgc ggttgtagat cggcttctcg ggtgtcgaat ccttggaatc gacgcagaag
     2581 tagccgtttc gaatgaattg gagatagtcg cccaccttgg ctttaagaac ctccggttca
     2641 gcttttgcat cttcgaccac aatcaatgat tccgggttca gatcgtccag aaagtttccc
     2701 gtttccttcc cgggttcttc ggcaaggaag agcttgtcgt acagccgaac ctcgattgat
     2761 actccttcgg cggcgctgac ccagtgactg gttccccgga ctcttcggcc atcggcggtc
     2821 tgcccgccgc gggattcagg atcgtaggtg cagtgaactt caaggacatt tccatcatcg
     2881 tcctttacga cggagtgggc ggtgatgtag tacgcataga tcagccgaat ctcagtgccg
     2941 atcgagaggc ggtgataccc tttggggggc ttttccatga aatcttcccg ttcgatgaac
     3001 acttcgcggc taaagggaac gaggcgggtt ccaccttccg gatcctcagg attgttgagc
     3061 gcttccacat attcgacctt cccttcaggg aaattgtcga tgacgacctt gattggatcg
     3121 aggaccacca tcagccgttt cgcccgtttg ttcaagtctt cgcggacgca gaactccaac
     3181 agggagtgtt ccacgattcc ttcaaccttc gccactccga ttctgcctac aaaatcaagc
     3241 atcgcttccg gcgagtagcc tcggcggcga ataccgctga tcgtcggcat ccggggatcg
     3301 tcccagccat cgacgacgcc catctgcacc agggccagca ggcggcgctt gctcatcagg
     3361 gtatagtcga tgttcaagcg ggcgaactcg tactgccggg gggtatgatc gatgaggtcg
     3421 atcgatgtcg cccagtcata tgccgggcga tggtcttcaa actcgagggt gcagatcgag
     3481 tgggtgatcc cttcgatcgc gtcgctgatc gggtgggtgt aatcgtacat cgggtagatg
     3541 caccagtcat caccggttcg cgggtgggtg gcgaacttga tccgatagag gaccggatcg
     3601 cgaaggttca tgttcggact ggccatgtcg atcttcgccc gcagcacata ggagccttcg
     3661 gggtgcttac cttctttcat ctctttgaaa agccgaaggt tctcttcgat cgggcgatcg
     3721 cgataggggc tgttttttcc cggttcggtc agggtgccgc gatactcccg catttggtcg
     3781 gggtcaagct cgcagacgaa cgcctgaccg gtttcgatca gatggagggc gatctcatac
     3841 agctgccggt agtagtcgct tgcatgatac tcatgttcac cccagtcaaa cccgagccag
     3901 cggatatccc gcatgatcgc ttcggtatac tccatctcct ctttctcggg gttggtgtcg
     3961 tctaggcgaa ggtggcagcg tccgccatat ttttcagcca gaccgaagtt cagggcgatt
     4021 gatttgatgt ggccgatgtg cagatacccg ttcggctcgg ggggaaatcg ggtgacgatt
     4081 tcattatgtg gaacccggcc gttggccaag tcatcttcaa tgatccgctc cagaaagttc
     4141 tggggctcag acctcatttc gcgatcgctc atgctcattc cttatggtgc gtgatatata
     4201 cttcgcctat agtagcatag acggtgtttt tgggcgagag tctctattcc gatgcggtga
     4261 accgaagatc ttcacccgtc gcctgatatg cttctccgtc ggagacgaag aacggaccga
     4321 agctgatcga agcggatgtc cccggaagtt caaacacacc gacccacgca acaggatcat
     4381 cctcttcgcg ttggaacccg atcatcgtcc gttttcgatc ccactgcatc cgccacttca
     4441 gcgtatcttt atcgcttgaa aagggataga ttacttttga agggtagcct cgaatcatgc
     4501 tgaatgtccg gatcgagtcc gcattcactc cgacggaggc gaagaccgta tcgatatggt
     4561 aggcacacag tcccacatag ccgccggggg gaatggaaag cctcatctca aaggtaaacg
     4621 gttcgccgct gactgcacac agcatcggag cattgggcag ttcggcgaca gggtgcgtgg
     4681 tgaggacaag ggaacgccaa tcgcgaaaaa cgcgggcggg aggtacaagg aaggtgaact
     4741 ctttgagttg aatccgtttc atggtcatca gtatactcac ggaacgataa tctttccata
     4801 acctctttat tgctctcctt cggtggtata tgcttgaacc aaaggagtca attcatgata
     4861 gctgttattg gagaagcgtt gattgatttt atcgggcatg gaacgggaag ccgaaccgta
     4921 ccgtttgaat cccatatcgg cggatgcggg atcaataccg ctgtcgccgc cgctaaagca
     4981 ggatccgccg tcggtttttt ctgcaagatc tcctccgatc tgttcgggaa gcgccacctt
     5041 gagcatctcg ttggaaacaa ggtgatgttt gatccaaccc tgtgcaacgc aaaggaaccc
     5101 tccttgttgg cgttcgcctc gctcgatgcg gtgggcaagg cgagctacgc gttctactgg
     5161 aaaaacagcg ctccggtatc gatgagcgtc gaggagctcc ttaagacgat gagcgaccat
     5221 accgatctca gggtcgtcca tatcggctcg ctcgcattga ccctcgagcc gatcggggag
     5281 gtgatccttg aagctctgga gcgctatgaa ccgaagccga tcatcttcct cgatcccaat
     5341 gtgcgcccat cggtgattga cgattggaat caataccgca cccacatcga aaaagcgatc
     5401 gacctcgctt cgtttgtcaa actcagcgat gaggacctgc agctgatcca tccggaaacg
     5461 gaagtgcatg ccaaggccgc cgagcttgcc cgaacaaaga agaagcacat catcctcacc
     5521 ttgggtcggg aaggagccgt ttggtacggt cctgaaggca aagaggtttc ccacccgatc
     5581 atcgatctcc cccttgccga taccgtggga gcgggcgata ccttcagcgg aacgattctc
     5641 tccttccttc atgaccgcgg ctacttcggg aaggagggcg agccgccgca acttctcgaa
     5701 attccgatca aggttcgtca agaggcgctc aaatgggctt ccgccgcctc cgccatcaac
     5761 tgcagccgcc ccggatgcga tcccccttca cggaaagagc tccttgcgct ccttgaaacc
     5821 ctctaggtgt tggaagtaga ctgtttttat gtctaaagac cagagaaaga tgaagaccct
     5881 gcttcgtttg atccgcttcg gaatcattct gagcctctca ttgttcatcg ccctttccct
     5941 gtactcatgg acgaagtcga tcggacggga caaacagcgg aaagagcttg ccgtactatt
     6001 gaagcaaacg gtggaacaag aaggtgttga agcaattctc tcgctcagcg gagtggcggt
     6061 tgagaatatc ttccacggtg aagaaggcct catcctcttt gaagggagcg atactccctg
     6121 gcgctacagc gcggacgagc tgcaaacgat ctccgtttac gaaaaggtga ataaaagcgt
     6181 tgtgaacatc accacaaaca cgaaccgttc agcaagtgat tttcttgacg tattgcccgg
     6241 tcacggaacg ggaagcggca tcgttctttc cagtgacggg tatatcctta ccaacgccca
     6301 cgtcgtggaa ggcgccgaga cgattatggt gggcctctac aacaaccaaa cgtaccaggc
     6361 gacgctggta ggggtggaca gcgaggatga cctcgctgtg gtcaaaatcg atgtgggaaa
     6421 ggatctcatg ctctacccga tcgcgctggg aacatcttcc gagttgcggg tggggcagaa
     6481 ggtgatcgca atcggaaatc ccttcgggta tgaccggacg atgaccatcg gggtggtcag
     6541 cggtctcaac cgccctgtgc ggaccggcga aggccgggtg gtgatgaacg cgattcaaac
     6601 ggatgcctcg atcaacccgg gcaacagcgg aggaccgctt ctgaactccc ggggagaggt
     6661 gattggaatc aacagttcga tcttctcgac ctcgggaagc agtcagggga tcaacttcgc
     6721 cattccgatc gacaccgccg tcgaggtggt cggggagttg attcggaccg gcaaggtcag
     6781 cagggggtgg atcgacgcct cgatgctgca gcttaccccc cgcctggtgg agtatgcgaa
     6841 actcccgatc agccagggcg tcctgatcag ccagctcgcc cccggcggaa acgctgaaaa
     6901 ggcggggctg aaaggaggaa cccggcaggt tcaatacggg tcaagcatct tctatctcgg
     6961 aggggacatc atcgttgcga tcgataacga gaaggtcgaa gacctcaacg gtctctacct
     7021 cgcactgctt cccaagaaaa gcggggagaa ggtcgccgtg acggtgaaca ggagcggcga
     7081 ggtgaaaaag atcgacgttg tcctcattga gcgaacgatg agccatatca gggcgttggt
     7141 gcgataagat ggatgaccga caggttgtag acggttcctg gggcggcgag acgatcacca
     7201 acgttcacgg cgaggtctcc tatcaggagc ggaggtttca ggtcataagc gagttcgagc
     7261 cagccgggga ccagggacag gcgatcgata aactgagtga gggagttctg gaggggcagc
     7321 ggtaccagac cctcaagggt gtgacgggaa gcggaaaaac ctacacgatg gccaagatca
     7381 tcgaacgtgt tcaacgcccg acgctggtat tgtcccacaa caagaccctc gctgcccagc
     7441 tgtatcggga gttcaagtcg ttctttcccg acaatgcggt ggagtacttc gtttcgacct
     7501 acgattacta ccagccggaa gcgtacgttc cgggcaaaga cctgtatatt gaaaaggatg
     7561 ccgatatcaa cgccgagatc gaacgcctga ggctttccgc atcgttttcc ctgatggaac
     7621 ggcgggatgt catcgtcgtt gccaccgtca gctgcatctt cggcctggta aaccccgttt
     7681 ccattcggga catggtttat acgttcaaaa gaggcgagcg gttcaatcac aaggagattc
     7741 ttaatcaact tgttcggatg cagtatgaac gcaacgacgc aatcctcaag cggggatcgt
     7801 tccgggtccg cggcgatgtg attgagatct gtccctccta cttggaaaat gcggtccgca
     7861 tctccgtcga ttgggatgaa atcgccgaga ttctgtggtt cgacccgatc agcggcgaaa
     7921 agcaggggag cgtggagacg ttctccctct atccggcgaa gcagttcgtg atgccccccg
     7981 aacaggtgaa ggcggcgatc gatcggattc gattggagat ggaggagcag gtcgagtatt
     8041 ttgaatcatt gggcaagccc ttggaagcgg aacgaatccg aacccgcgtc gagtatgatc
     8101 tggagatgct cagcgaaatc ggatactgct cgggaatcga gaactactca cgccagcttt
     8161 ccaaccgcaa ggccggggag cgcccggcgg tgctgctcga ctacttccct ccggacttcc
     8221 tcaccttcat cgatgaatcg cacgtaaccc tcccgcaggt cggggcgatg tatgaaggcg
     8281 accgctcccg aaagctgaat ctggtgacct atgggttccg ccttccttcc gccctcgaca
     8341 accggccgct gaaagcggag gagttcgagc aggtgatcgg acagagggtc ttcgtctcgg
     8401 cgacccccgg gaagatcgag ctggaaaaag cgaccaacgt ggtggagcag ttgatcagac
     8461 ccaccggtct gctcgacccc aaggtcgagg ttcgcccaac cgagggtcag atggaggatc
     8521 tctacggaga aatccgggca accgtggcga agaaccagcg ggtcctgatc accacgctga
     8581 cgaagaagat gagcgaagac ctgaccgact acctcagcgg tctttccctg aaggttcgct
     8641 atctccactc cgagatcgag acgatcgaac gggtcgagct cctcagggat cttaggctcg
     8701 gcgtcttcga tgtcttggtc ggcatcaatc tcctcaggga agggttggat cttcccgagg
     8761 tttccctgat agcgattctc gatgcagaca agatcggatt cctgcgctcg gccaccagcc
     8821 tggttcagac gatcgggagg gcggcgcgaa acgccgacgg acgggtcatc atgtacgccg
     8881 accgccacag cgatgcgatg atcgaggcga tcagggaaac cgagcgccgc cggaaggcgc
     8941 aacttgccta caacgcggaa catggaatca ccccgaagac gatcatcaag gcgatcagcg
     9001 acatcatcga gcgcgagaag gaaacgaccc aagcgattca aagccgggag atcgagattc
     9061 tcaagggagg gtacaacctt ctgctcgcct cgggacgcaa gaaatatgtc aaagcacttg
     9121 aaacagagat gttcgaagcg gcaaaggatc tggagttcga acgggcggcg ctccttcggg
     9181 atgagattca gcggatcgaa cggggagaga tcgaatagcg gagaacgagg tcgagagccc
     9241 cgccgctcac gatcggatgc tgatagaggt gcttgttcga cccttcggtg ggaaacccct
     9301 ggtgttcatg gatatggtga tagacctgat cgagggtgac ctgcttactt gttgtgtcca
     9361 gtgcctccaa caacgctttg gtgaagtagc cgtgaatcgg agaaccgaat ctcggctcct
     9421 tgctggtgtt gtccgccgtg gtcgccgcca agacgaagag gcggggattc cgttcgatgc
     9481 cctgacgata tccatcccgg agattttcga accggttctt cgaatcgatc gtcgacacgt
     9541 cgccgctttc ggggatgaag gagccggcgt agcaggagtc gaggatcagg aggattgtcg
     9601 cttcagagcg atcgaataaa gagatcagtt catccacccc gagcaaggct ccatccagat
     9661 tgtcatccaa aagcaaccca ttactgttgt ccggagccag aacgagcgaa ccgtcttcga
     9721 ccgcatgccc gctgtaggtg atgatgagta gatcgttctt cttcatgccc cccagtgtcc
     9781 gaatgatcgt ttcgatgacg aggtctttgg taggaagatt cttttcatcg agggtatagg
     9841 cgcctttctc atccccttgc tggagcagaa gggtggattc aaacggttcg gcagagaaga
     9901 aggagtggaa gctctttttc aaggcgaccg catcgtttat cggaccttcg aggacaagga
     9961 gatcggtgcc ccggtagctg atgccgattc caatgtgatg gatgttccga gcggattcaa
    10021 agggaatcag ctcgcatgaa gcgagcacga ttaccgcgag aaaaagccac agaaacggtt
    10081 tcataccccc taaggggcaa aagaggcggt ttcgcatcaa agcctgaaca ggatgaaatc
    10141 catcgccccg ccgttggtca tcggatgctg gtacatcgac gccggtcgat agatcgtggt
    10201 ccgaagagga agcttttggt gttgtttgat ataccggtag agcgaatcga cactgatcac
    10261 accattgtgc tttgcaggta tctcagtgcc catgagaaat ccgtcgtctt cgtcatgaga
    10321 gccgcccagc gcctccagaa gcgctagggt gaagacgcca tggatatggt cgtctcccat
    10381 ctcataggag tcggtatgcg ccgtcgcggt gaacaacgtg accgggggaa tcgcataggg
    10441 cttatcgctg aaataggtat cgaaccaatc ggaaagcgag ggggaacgaa ccagactgac
    10501 cgaggattcg gaagccggca cgctcattcc actttcacag gaatcgagga tcaccagctt
    10561 gcggcctttg atgggctgca attccttgag aagcttttcg gtaaccttca gttcaatgtc
    10621 atagtcgtca ttggattcgg gggccatgaa gagcacgtct tccccgccgt gaccatggta
    10681 ggtgaggatc agcaggtcct cttctttaat tgcatcggtc aatgcgtcca acaataaggc
    10741 gagattcgct ttcgacgcgt atgaacggat tggtgttcct ccgaccgtaa tcatttcata
    10801 cgtggattga tccttagtat ctccttcctg atacatgaga tacgagtcca tctcaagctt
    10861 tgccagacct gcaatatggc cgaacgccgt tttcatctca cgggcatcgt tgagggtgcc
    10921 cttcaggggc agcaagcggc cgtattcatc agcgggattc gtgaacgtcg tattcctgta
    10981 atccaacccg atgaacacgc catagacctt tccctgaggc ggctggtctt gaaccagctc
    11041 gcaggagaca agcagcagga tgggaagaag gaggaagagg atggttcttg ttttcatagc
    11101 aagcctcctc cgtagggatc gaacgaccag cgtaagccca aaccgattga aagggcggtg
    11161 atctccttcc tcaggtcgat cataatcgga aggatcgccg acaactccgt attcgttgtt
    11221 tcaagaatcg tgtactgaag acccgcctcc gcccagaagg agaggaacgc ccattcgatc
    11281 gccgtatagc ggttgaccgc tattcctccg ctgccaaaga gtgcgaagcg gtcagtgaga
    11341 ctgtaagaga agcgggcacc gagcccgagg gaggtgaacc ctcgggcgcg ataggatccc
    11401 caagggaggg agggggtgac gaacgcccct ttcaatgatg cgttgaccga ccaggtatcc
    11461 gcctcatagc cgaacggttc gaagatgaag gtgttgctca ttgaggtgcg gaagggcagt
    11521 tcatcctcga aatggggaat cgaaagatac gcgcttggaa cgtaggagat cgtcgtgacg
    11581 aagttcccgc cggcgatcgg gagggcgaat agcagaagga gagaaagaac caacggcctc
    11641 ttcatggaag tagtataccc ttttcccttt gaatggccat gcccctcttg ggggatatga
    11701 agaatctact cttcaaagta tgaaagatcg gtgatgtcga catgcaggga aatgatctcc
    11761 ttttttggtt tccggtcgct gtggcagagc agctcatgct cattccagag acaactgttc
    11821 cgccagtggg tcccttggaa ttcgctgcga atcacgcgga cctcgtcgaa ggcgaggaat
    11881 ggcaggtact gaaggcggac ttctcccgga tcctccacaa tgtggcttgc atggggtcga
    11941 aagaagaggg aggtgttggt cgggttccgg tgttttgatg gtatttcgag ttggtagggg
    12001 ttcctcagcg tccgggcgaa cgcactggcg ggaaggatcg ttccttcccc ggtgaacgtg
    12061 gcgacaaact tcgtcctggg ggttcgatag atctcctcgg gggtcccctg ctgaatgatt
    12121 cgcccctgat ccatcacgat cacctgatcg gcgatcgcaa gcgcttcatc ctggtcgtgt
    12181 gtgacgtaga tcgtggtgat tcccgtctct tcatggatcc ggcgaatttc ttccctcaag
    12241 cgggttcgca gctttgcatc gagggcggaa agcggttcat cgagaagcag tattttcggt
    12301 tccgaggcca acgcccgtgc caaggcgacg cgctgacgtt cgccgccgct caagcgggtg
    12361 cttttccgtt tctcatatcc ctgaagattc accagctcaa ggagttcctt gaccgcctgg
    12421 acccgttgcc gcttcttcag cttcaggttt ttcaggggat aggcgatgtt ttgttccaca
    12481 ttcatatggg ggaaaaggga gtaatcctga aagaccatag cgatctttcg cttgtgcacc
    12541 tctttttggc tgatatcttc gccaccgaga atgatcgatc cggtctcggc gtggatgagg
    12601 ccggcgatca tgctcaatgt cgtggatttg ccgcatcctg aaggaccgat gatgcatacc
    12661 agatcacctt cctgaacggt gaaggcgaga tcgagggtga aatccggata ggtggcttga
    12721 agggaacatt ggaggatcat acggaatctc ctttggcaac ttcaccaagg aagaaggtaa
    12781 ggccgctggt gatgatgagg attgagccca aggcgcaggc gccttggaaa tgatacgagc
    12841 cgatgaggcg gtacatgacc accggcaggg tcacgatccg gctgcttgag aggatgaggg
    12901 tcgcattgaa ttccccgagt gacagggcga aggcaaagac cgccgccgtg agcagcgttc
    12961 ttctcaaaag cgggatttcg acggtacgga acgttttttg aggtgaggag cccaaggtat
    13021 aggaagcgtg cagatatgtt tccgagaggg aacgaacgtt tgggagaatg gttctgaggg
    13081 cgaacggaaa ggcgatgacg gtgtgggcaa gggtgatgag cagatacgtt ccgaaaagcg
    13141 acggtcgaag aaggcgggcg atcaacacgt accccagccc gacgatgaca ctgctggtca
    13201 tcatcggaag catcccgaag agctccaaaa aggatgagct ctttttccga cgggcgatcg
    13261 aaacactcaa caggagagcc aacgtcacgg tgaggatact taccgccact ccgatgaggg
    13321 tcgaattgat caaggcggtc aaggcactgc ccatgtgact ggaggattgc tcaaagccga
    13381 ggagctgtcg ataccacgtg gtgctgaagg tcgtctcgct tgcgcgtgaa gctccttttg
    13441 agaaggatcg atagagaacc gagataatcg gaccgagtaa gaagatgatc gtgaagaggg
    13501 agtagaggag gatgaaaaag gttctcatcg gcgaaggttt tcgctcctca aacgctcgct
    13561 cggcaaccgt ttcggtaacc gtaagagtcc gctggctgaa agcgtatccc aagaagagga
    13621 ttccattgat cagcatcgag acaatcgcca aggcgctggc gcttgaagga tcgcccatca
    13681 tccgggcttg gcggtagatt tcaacttcca gcgtggtgaa ttgaggtccg cctccgagga
    13741 cgaggatgat ggcgaaggag ttgaaacaga agagaaagac caaggcagca gcgctgatga
    13801 tcgaggggac gagccgaggc agggtgatcg ttcggaacac tttacgggga gctgatccca
    13861 aggtcgccgc tgcagcttca agtttggtgt caaggccttc ccaggcggtt gcgacgatgc
    13921 tcatgatgat cgggaagttg taaaacgtgt gagcaagaat aatcgctcca agggagtaca
    13981 gaagcttcag cgggggatcg tcgaggtcaa agaccttcat cagaaccata ttcaacgttc
    14041 cgctgtttcc aaagaacatg acaaatccca agaccacgag aattgatgga agaacgaagg
    14101 gaattgtcgc caacgccttg atcagcttct tgcctcgaaa ggtgtaggtc gccagaatat
    14161 acgctcccgg caacccgatg accagagcag cgccggtcga atagaacgcc tgcttcaggg
    14221 tgaagagaag gattcgctgc gtgtagggat ccaagatcgc cgaaacgatc cgcttcaagg
    14281 tgaaggagcc ttcttcccac acccctgact gaatcgtcag ggcgagggga agggcgaaca
    14341 gcgagaagag gatgaccagc ggtccgatgc tcttctggcg atagaatcgc cgataatcac
    14401 tcatcgactc atgacctccg tccattcatg caaccatcga tcgagttttt tttcaatcac
    14461 ttcactctcc attcggagcg aaacccccgg cttgggagca taggcgaacc cttcggtgag
    14521 agctgtgttc atattggtcg gatacattga gttcgccaac gcaatcggaa gctgcgcttc
    14581 gcttaagaga aaatcgatga acagcttcgc ttcctccttt cgttttgagg agcggagcat
    14641 gcccaccccc tcgatcgttg cctggtgtcc ttcatcaaag atgagggctt gatagcgggt
    14701 ggagccttca taggtgtagt gatagaccgg actcgtggta tagcttaaga cgatgggagc
    14761 ctctccttca atgaagagtc cataggcgcc ggaccacccg tcggtgatgg taagcacttg
    14821 gtccttcatc gctttccacc aatcgagcca gccttcttcc ccgaagatgt gaatcgtcca
    14881 caggagcaac ccgagaccga cattggaggt cctcggatcg atcaggatga tgttcttcct
    14941 gaaacgagga tgagtcaaat cttccaatga tgtcggccaa tcttctttgg gaatccgctc
    15001 ggagtcgatg atgaaggcga agttgccgta atcgaaaggc aggagccggt attcggaatc
    15061 gaagtggaga aattcgggta tctcatgaag cagcggggag tgatagggct cgaagagatc
    15121 ggcccggtac gcccgggtgg ccatttcatc gctgattccg atgacgacat cggcataggg
    15181 atgatcttgt tcggcgatca ccttcgtgag catctccacg ccgtcgccgg cgctgatcac
    15241 cttcacgggt atatcgtgtt ttgcctcgaa tgcggcaacg acctcttttc cgggacccca
    15301 gtcgccgctg aatgagtcat aggcgtagac agtgagctgt tgctcctttt gtgcgcccat
    15361 cgaaaaaagg ggaaaagcga ttgtcagcgt gatgcagaca ccaagaacga atcgatgcat
    15421 cttcatatca gcacctccgt aggatgtagt ctgccttggg tgaaattgag atgggagtct
    15481 ccctccgctg gtattaccca gatcaggtta acgggtatga tctcagacct ttcggccacc
    15541 ccaggacttc cattagctta gaggtgccga gagtcgatgt caagcgttgc cggggtgtaa
    15601 aatgctacac aaaaaccttt cgcttggagg tgaacgtata agggcttcag ttgttgcaaa
    15661 ttatgcaaca actgctactg atgggaaaat ctatcaggtt tcagatagaa cagagaactt
    15721 acagactttt gctagatatc tggcactggt cttgcccaca ccaacgcctc cctcgtcatc
    15781 aataagggca tgatatgggt tatt
//
LOCUS       DAOSLI010000041        15776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_3893_length_15776_cov_26.360092, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000041 DAOSLI010000000
VERSION     DAOSLI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 15776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15776
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3893_length_15776_cov_26.360092"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..892
                     /locus_tag="PLF67_06685"
     CDS             14..892
                     /locus_tag="PLF67_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOE89734.1"
                     /translation="MSKRIVILLIVLLPALLFAGGKKERVSEKPVVVVSILPHAFFVE
                     ELGGSDVDVLTLVGEGQSPHSYEPSPSQMARLSKARVWILSGTEFELALKPKIQSMYP
                     DLVLIDGTDGMVFRQLEEGHHHDHDSLNIDRHTWLGHDQVKVFLGHALAALTDLLPEN
                     GTAFEERHRVLVDRIDELFAELKEDLAPLSGQPVFVYHPAFGYLLDDLGLIQVAVETG
                     GKEPTAKDLASLVELAKREHPKAIFVQKQFPVASAQAVADAVGSAVVPLDPLAYDWLA
                     NIRTIGDALLESGGRK"
     gene            892..1668
                     /locus_tag="PLF67_06690"
     CDS             892..1668
                     /locus_tag="PLF67_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89735.1"
                     /translation="MIEAPVALQFKGVSFAYPSLQILEDVTFHFHQGEFIALVGPNGS
                     GKTTLLKLILGLEEAQEGTITLMGGSVKTMRPEIGYVPQHATYDPSFPISVIEVVMMG
                     LVDSTRRLKKQEIRSEAIQALKQVELEDLVDRPYSQLSGGQRRRVLVARALASSPRML
                     ILDEPTANMDKESEGRLYSTLERLKGNTTILIVTHDTRFVSDLTDRVFCIDTKPEGGT
                     GGRTVVQHALESVEGEDQKRVRHDVEIPADFCAYPRELPS"
     gene            1665..2534
                     /locus_tag="PLF67_06695"
     CDS             1665..2534
                     /locus_tag="PLF67_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOE89736.1"
                     /translation="MNGFFEILFSPDVPFIRNALFAGILSSILFGILGSVVTVKRIAG
                     LAGAISHAVLGGIGIALYLSATGIAPNLSPMVGAIIFALLSALLIGAVSLKSKQREDT
                     VIQAIWAIGMSIGVLFMAKTPGYTDPSSYLFGNILLISGRDLILLAILDVIVLFLAWR
                     FYPQIEATSFDEEFAAVRGIPTQTVFLIILSVTAVAVVLLQTFVGIVMVIAMLTLPAG
                     TAGYTARNLASMMLFSTLYSLLFSAGGLVVGWSLDLPVGAVVVVLSGLVFLGFATATV
                     IRKKRSSRGPSSD"
     gene            complement(2494..3432)
                     /locus_tag="PLF67_06700"
     CDS             complement(2494..3432)
                     /locus_tag="PLF67_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE89737.1"
                     /translation="MLILSDILLFLTAALVFSVTCLVILLRVRVADALTTDFLRVLVP
                     LSLQMLLSLLATYLERSLSPRQLASFSYTMYALGATIISILLTTAMLFAFTRFFIGRL
                     PASEKQKRLGNIILRLLIILFFLISIWAILGKSRGDWIKALDETMKYHFFSASTFLVI
                     HAVVALFFIKQASTWEEEWLLKGVAMTFLPLVVLFPLDILFFLHLSFKVVYLSFAILS
                     VHLYFFISRRYFLTYEQPALPKVNLTERFGITEREQEILRLLADGLSNQEIAKQLFIS
                     VNTVKTHIKNIYAKLEVNNRLQLFNLMKDRDSNASS"
     gene            complement(3470..4879)
                     /locus_tag="PLF67_06705"
     CDS             complement(3470..4879)
                     /locus_tag="PLF67_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89738.1"
                     /translation="MKKLLVLILLIAFVSPLFAFDFFATLDDEEGQVGTEPAKPKELT
                     SLFVEGFGTYYPLDEDEPFDYGISLGVTFEENRDKIRTEVDFSFSPILRDPLDVRTLA
                     VTFFFGPTNLKVGMFTHPWGTSTTLHAVDILNSQNLTHGILDNPEAMKKSEFMIVYNA
                     YFKRSSLDLIIKPFYRPIDMEASDRYAPMDTDNLMGTSFSYGGMEIPIDSDNIRVGIY
                     QHPTFWKALGGGGRYRFGIGDLDLGFMYFSSFYPQMGFDIGAKLKMKPHPLDPTQYIP
                     DEVEVTSIDFDFTRYHFAGTEGSLLVGPVTIAWEGGLFLSEDVKGEKPEKYNTKIAAV
                     VELSYTEPKTSFFASAAYQGQYLFGDVTPTVNQTLDVIIPVDYIASFLSDKPYSSSVA
                     VAFEMPFMRERLKVRMAATYGIENQGLVALGSLSYEINDSFSVYARGAYYNGFGDKKS
                     LFKNWENNSSVTIGMNAWF"
     gene            complement(4884..5639)
                     /locus_tag="PLF67_06710"
     CDS             complement(4884..5639)
                     /locus_tag="PLF67_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOE89739.1"
                     /translation="MKRVFIMLLLILIPALIIAAPSAALIMDKVIETQKADTAAMDLK
                     LTLIEANGTERERRIQTLSQTIDDKTSIITVFLSPESVKNTRFLSVEGNDATEQWIYL
                     PALKRVRKISATEEGGSFMGSDFSYSDMASTTYDADEATHRLIEETATTYVVESIPKT
                     VKSYGKNIATVDKETFLPLKVEFYAPDEKTLLKTLTTLETGTVGTRPITKMVEMKTIA
                     TGHATRLEMVQVRYDVPIPAAYFTVRFLETGRL"
     gene            complement(5636..8014)
                     /locus_tag="PLF67_06715"
     CDS             complement(5636..8014)
                     /locus_tag="PLF67_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOE89740.1"
                     /translation="MEKRRTLDWIIISLSFVLTVLGLLGIGWLHVDSSTDAFIPTNAD
                     VVQINDRIEERFGSLDPVVVSIYAEDTILQPKYMEILKTITEEIEKLDGVSTANSIAN
                     VQHLEPSFDGVEAVSLYDGDFEALKNRIEEWPSFYEGTFLSADHTVASVLIQTTLDYD
                     SKQLLHDLREVVAPYQGVVETSILGLPVVTEAISESLLSDLGILVPIVATLIILILYY
                     FLRSFKATLLSLVPLIFSSSVALGIMAIAKITFTMATMLVPVLLLIVGSAYTIHIFSH
                     FFQNQDSGTIEEVLRVVVKRNTYPIIAAAATTAFGFLAQLSSPLGPFRIFGLLSFIGV
                     VICAFSSLVLLPALIRVVYKQPNGKERKKQQNKDMRLMTAAMVVANHHGKALLWVSMI
                     ILVIFLPLSYYFVESGTNMLDFFRPSSSLVKDSNRFNERMQGSFSMTVTIEPGEDISI
                     LDPSTLKTIEAAIRRLEAEPVVGGVQSIIPFVKRMNQLLGPGEDEIVHTVDEEEVVFD
                     FFAFDDDFFLGFEEEPVEAQEVVASAGGQGEYEIPGDPARYGLADESELANLIAQYLL
                     LYSGNLDMFINDPLEPDATALTLVLKSSDTNRLRTITNLISTLFPEDWKVEVGGGEAV
                     ALALADLVTRSQIVSLFSSLIAVWFLVWFTLKSPKLATLALIPCLFALLAVFVSMLAF
                     SIKLDIVTSLLAALCIGIGVDYAIHLLSAIVRNDMPMDRIMQTTGKAILANAASVALG
                     FIGLLFSRFMPIANLGVLFCIAMATAAASALLLLPAIAVHYPKLMFTRRTKS"
     assembly_gap    8318..8417
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8418..9428
                     /gene="pyk"
                     /locus_tag="PLF67_06720"
     CDS             <8418..9428
                     /gene="pyk"
                     /locus_tag="PLF67_06720"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOE89741.1"
                     /translation="CRMVEEGTILPRKGVNLPGVPLVHLSSFTEKDKADLAFGFKNKI
                     DYVALSFVRSGDDVALLKKYMTETFGHAIPIISKIEKPEAVDNIHQIMDESSAIMIAR
                     GDLGVEVPAEEVPLIQKSIIQMGINRNLPVITATQMLESMIYRPRPTRAETNDVANAV
                     LDGSSAVMLSGETAAGGYPLQAVRTMANITSMIEQSEVFKREVMNQILGMDMLRKQTV
                     TEAVGLATRDLAISVKASYITCFTHSGSTARLIAKFRPSVPILAFSPNKSVVQYLCLS
                     WGVTPILIDELESVDELLLFAPEYLKKNGLVKTGDAVVITAGVPVGSSGKTNMIKVVE
                     IE"
     gene            complement(9429..10178)
                     /locus_tag="PLF67_06725"
     CDS             complement(9429..10178)
                     /locus_tag="PLF67_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89742.1"
                     /translation="MKRTLLIVGILLLGTALLLAEPTLNGTSGYIIAPSANVEESGPK
                     LAIATGYSMIYGDKGLAHVPFIYLSFPIDVEVSFAVDTKTATDLLLSAKWRFAEKRGT
                     SFAVGANLQLIEVGDSNTFGVQGYLASTFRHTLMDLPTKSSIMVGYSWKQGTQALSNI
                     DFAVGLETMLFPSKLKNHLALLLDFGNISYSLSPSGGNAQNRGLVNVGLRVPAFKLFP
                     STTISFDVRALDLFDHAGRAVSFSTALTFRP"
     gene            complement(10175..10957)
                     /locus_tag="PLF67_06730"
     CDS             complement(10175..10957)
                     /locus_tag="PLF67_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOE89743.1"
                     /translation="MKRKNNAIYIDLENIPSTLDLKSLLDDLSLRHNVDSDEENVFVI
                     KMAAGNSTAIRKMKKQLTEYNFEIRDTPSITSKYKNRADLIISLAALETIIINMPVID
                     RYVFITSDSDFTVIMEMLRKYGKEVFLVTKEEVSDKPVFSNSCDEILILESYTTKTKG
                     AKKETKVSVEPTPQEKMNDQVKALAKKVIDSFDPDSWQLASFVGTKFHQMDKSRMIER
                     SKYKSLGALLAQLEKEKYITKRINEKGQPELRKATEEPRSPL"
     gene            complement(10987..12204)
                     /locus_tag="PLF67_06735"
     CDS             complement(10987..12204)
                     /locus_tag="PLF67_06735"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE89744.1"
                     /translation="MINIFDEEPARLHARYLAHLITKTGFLDFDSGKTLPVKEHGGVM
                     IGVLLGTDRYGNEVLLKAISGQQRNLARYVPHVITDTDFQTHLDTYDALIKNPHNTPF
                     NKSEYSAQALNHYYGLYRFPTIDGSTVSLFECFGNRPIPTGSGDCAAIKLLAYALGNH
                     IVPTSMTEFFYGDGTRSHLGFYQPCEERCAPILKRMLGLYPVYRDKHLLIVDKPPHLR
                     SIPGTVESDSIESRVRKLIPSAPVQCAAHRLDMDTSGLIVVALTKEALSGMHHLFRTQ
                     RVRRTYEALLEGILKEEETEISLPLRPDITNRPYQVVDHEGGKEARTHVKRIRVEEHP
                     EGHLSRVEFTPYTGRTHQLRIHSKKGLGLPILGDRLYGSGMKSRLYLHAKSLEFTHPI
                     TKEEIRVDGPVPF"
     gene            complement(12197..13540)
                     /locus_tag="PLF67_06740"
     CDS             complement(12197..13540)
                     /locus_tag="PLF67_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOE89745.1"
                     /translation="MATQTRLMTAGSIRKHLIFFAFPIFLGGLLQQLYHTTDAVVVGN
                     FVSTQALAAVSSVGSLTMLIVGLFQGVFVGSGVVISSAFGQGDYDSVTKAVHTTVALG
                     IISSVLLTIIGYTFTPAILLWMKTPPEVFTDAQTYIQIFFLGVSSLVLYNTASGILQA
                     VGDSKNPLYFLIISTILNIVLDLVFVISFGMGIEGTAYATIIAQSVSAILAFRLLIRT
                     KEVIQVRIKEIRIYRGYLGKTLRLGVPSGIQNSVTSFANVIVQSSINLFGPAAMAAHG
                     SYNRIQGFAFIPITAFALALTTFTGQNLGAGKQDRVRKAARFGIFFAMALAQLVGFAL
                     MIFGGQVMTIFTRDSEIIRLGVQKGLIAGWFFWALALSHTMTGLFRGAGKSIVPMTVM
                     LSIWCVLRIMFIKVGLALFFDIRIVFWAHPLTWAISSIIFIIYYFTADWMKRSDD"
     gene            13725..13859
                     /locus_tag="PLF67_06745"
     CDS             13725..13859
                     /locus_tag="PLF67_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89746.1"
                     /translation="MKLELIRHVHVSEEYFTGAGKYHHGNQEAIEVQMKAVANQGKAK
                     "
     gene            13939..14991
                     /locus_tag="PLF67_06750"
     CDS             13939..14991
                     /locus_tag="PLF67_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE89747.1"
                     /translation="MAFPWGNKELVSPPGKTIRDLIEDRGMTQKEFATRMDLSEKHVS
                     KLISGEVLLTTDVILRMISVIGKDVLFWSNLEALYRAGVVRETLKDQVALDERILPRI
                     PLKELSDLGWIDLDDRPEAQVELARQYFEVCTLQIIESESMRSYTSYGAYIDREMDYT
                     LLAWIQQAKFRARSMGVRPLDHRKLERNMMKVRSLSLLSPKEFLPKLQGLLAHCGVAF
                     VVLPAMKELRLEAITFFAEGRATIAFTAEGKNSETFWISLFRELAHILLRHVGPRIVS
                     SKDAISEAESLGFELLLPSDRWKTFLARKRFDEKAILTFAQEEKIHPSLVANRLGNSK
                     KELAHLTVAYELLEAI"
     gene            complement(15069..15515)
                     /locus_tag="PLF67_06755"
     CDS             complement(15069..15515)
                     /locus_tag="PLF67_06755"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOE89748.1"
                     /translation="MKRLPYHGAGILAWRKDAHGKTEVLLGLRKYNPQANMWSIPGGE
                     WDERKDGFEEGTTPDYRATAIRETWEEIRLSIDDGDDLALLWRTHIPFYHFRVFAYPM
                     PEKRNLVCHEEFSRLRWFPVDDLPNNTVSLVRLQVSALIKDSGDGA"
     gene            complement(15562..>15776)
                     /locus_tag="PLF67_06760"
     CDS             complement(15562..>15776)
                     /locus_tag="PLF67_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89749.1"
                     /translation="ERVDIVAFAFSPGGVKKGCPLDRLDGRKGYITIDDALARNWRVF
                     DEDTDELLGTYRSSAEVVAGGWKVST"
BASE COUNT         3658 a         4029 c         4458 g         3531 t
ORIGIN      
        1 cgagggggaa atcatgtcta aacgaatcgt tattctattg atagtgttgt tgcccgcttt
       61 gctctttgcg ggtggaaaga aggaacgcgt ttccgagaaa ccggttgtcg ttgtgagcat
      121 cctgccgcat gcgttttttg ttgaagaact gggtggctcg gatgtggatg tgctcacctt
      181 ggtgggggag gggcagagcc cgcactccta tgaaccctcc ccatcccaga tggcccggct
      241 ctccaaggcc cgggtctgga ttctcagcgg aaccgagttc gaactggctc tcaaaccgaa
      301 gatccaaagc atgtaccccg acctggttct catcgacggg acggacggga tggtcttcag
      361 acagcttgaa gagggtcatc atcatgacca cgattccttg aacatcgacc ggcatacgtg
      421 gctcggacat gatcaggtga aggtgtttct cggacatgcc ctcgctgccc tgaccgatct
      481 ccttccggag aacggaacgg ccttcgaaga gcgtcatcgg gtgctggtcg atcggatcga
      541 tgagctgttc gccgaattga aagaggatct ggcaccgctt tccggtcaac cggtgttcgt
      601 ctaccacccg gccttcggat atctgttgga tgaccttggc ctgatccagg tcgccgtcga
      661 gaccggaggg aaggaaccca cggccaaaga ccttgcttcg ttggttgagc tggcaaagcg
      721 ggaacatccc aaagcgatct tcgtccagaa gcagttcccc gtcgccagcg ctcaggcggt
      781 cgccgacgcg gtgggaagtg ccgtcgtccc cttggatccc ctggcctatg actggcttgc
      841 caatatcaga acaatcggcg atgctttgtt ggagtcggga ggaaggaagt gatgatcgaa
      901 gcgccggtcg ctcttcaatt taaaggggtc tcgttcgcct atccctcatt gcagattctg
      961 gaggatgtca cgttccactt tcatcaaggg gagttcatcg ccctggtcgg ccccaacgga
     1021 agcggcaaga cgaccctgct gaaactgatc ctcggtcttg aggaagcgca ggagggaacc
     1081 atcaccctca tgggcggcag cgtgaagacg atgcggcctg aaatcgggta cgtcccgcag
     1141 cacgccacct acgatccgtc ctttccgatc agcgtcatcg aagtggtgat gatggggctt
     1201 gtcgattcca cccggaggtt gaagaagcag gagatccgaa gcgaagcgat ccaagcgttg
     1261 aagcaggtcg aactggagga tctcgtcgac cgcccctaca gccagctttc cggagggcag
     1321 cggcggcggg ttcttgtcgc ccgagcgctg gcctccagcc ccagaatgct gattctcgac
     1381 gagccgaccg ccaacatgga caaggagagc gaagggcggc tgtactccac cttggagcgc
     1441 ctcaagggaa acacgacgat cctcatcgtc acccacgaca cccgcttcgt ttccgatctg
     1501 accgatcggg tcttctgcat cgataccaag cccgagggcg gcaccggcgg ccggacggtc
     1561 gtccagcacg ccttggagag cgtcgaaggg gaggatcaga agcgagtccg ccacgatgtc
     1621 gaaatcccgg ccgacttctg cgcctacccg agggagctgc catcatgaac ggtttttttg
     1681 agattctgtt ttcacctgac gtcccgttca ttcgcaacgc cctcttcgcc ggcatcctca
     1741 gctcgatcct gttcggcatc ctcggtagcg tcgtaacggt gaaacggatc gccggccttg
     1801 ccggagcgat cagccacgcc gtgctcgggg ggatcgggat cgccttgtac ctctcggcga
     1861 ccgggatcgc ccccaacctt tcaccgatgg tcggggcgat catcttcgcc cttctttcag
     1921 cgcttctgat tggagcggtc tcgctgaaga gcaaacagcg ggaggatacg gtgattcagg
     1981 cgatctgggc gatcgggatg agcatcggcg tgctcttcat ggccaagacc cccggctata
     2041 ccgacccttc cagctacctc tttggaaaca tcctcctcat ctccggccgc gatctgattc
     2101 tgctggcgat cttggatgtg atcgtcctct tcctcgcctg gcgcttctat ccgcagatcg
     2161 aagcgacgag cttcgacgag gagttcgccg ctgtccgggg gattcccacc cagacggtgt
     2221 ttctgatcat cctgtccgtt accgccgtcg ccgtggttct tcttcaaaca tttgtaggaa
     2281 tcgtgatggt gatcgcgatg ctgacccttc ctgcgggaac cgccgggtac acggcgcgca
     2341 acctcgcatc gatgatgctg ttctccacgc tgtactcgct tctcttctcc gcagggggac
     2401 tggtcgtggg gtggtcgttg gatctgccgg tcggtgcggt cgtcgtcgtg ctctcgggcc
     2461 tcgtcttttt gggcttcgcc acggcgacgg tgatcaggaa gaagcgttcg agtcgcggtc
     2521 cttcatcaga ttgaacagct gcaggcggtt gttcacctcc agcttcgcat agatgttttt
     2581 gatatgggtc ttcaccgtat tgaccgagat gaagagctgc ttggcgatct cctgattgga
     2641 taagccatcc gccaggaggc ggaggatctc ctgctcccgt tcggtgatgc caaaccgttc
     2701 ggtcaggttc actttgggaa gagccggttg ctcgtaggtg aggaagtacc ttctgctgat
     2761 gaagaagtag agatggaccg agaggatcgc gaagctgagg tagaccacct tgaagctgag
     2821 gtggaggaag aagaggatat ccaagggaaa gaggacgacc aatggcagga aggtcatcgc
     2881 gacccctttc agcagccact cctcctccca ggtcgaagcc tgctttatga agaagagcgc
     2941 cacgaccgca tggatgacca gaaaggtgga ggcgctgaaa aaatgatact tcatcgtttc
     3001 atccagcgcc ttgatccaat cgcccctgct cttcccgaga atcgcccaga tggagatcag
     3061 aaagaagagg atgatgagca ggcgcaggat gatgttcccc aaccgtttct gcttttcgct
     3121 ggccggcagc cggccgatga agaagcgggt gaatgcaaag agcatcgccg tggtaagaag
     3181 aatcgagatg atcgtggctc cgagggcgta catcgtatag gaaaagctcg ccagctggcg
     3241 aggagaaagg gatcgttcaa gataagtagc gaggaggctt aacagcatct ggaggctcag
     3301 gggaacgaga acccgtaaaa agtccgtggt cagggcatcg gcgacgcgga ctcgcagaag
     3361 gatgacgagg caggtgacgg aaaagaccag agccgcggtc aaaaaaagca ggatgtccga
     3421 aagaatgagc atatgccctc cgttcggtca tcctaccatg aaaaaaccgt tagaaccaag
     3481 cgttcattcc aatcgtcacc gagctgttgt tctcccagtt cttgaagaga ctcttcttgt
     3541 caccaaagcc attgtagtaa gcacctcggg catatacgct gaaggaatca ttgatttcat
     3601 agctgagcga tcccaacgcg acgagacctt ggttctcgat tccatacgtc gccgccatcc
     3661 ggaccttgag ccgttcgcgc atgaacggca tctcgaaagc gaccgccacg ctgctggagt
     3721 agggcttgtc gctcaggaag gaggcgatat aatcgacggg gatgatgaca tcaagcgtct
     3781 gattaaccgt cggtgttaca tcaccgaaga gatactggcc ctggtacgcc gccgaagcga
     3841 agaaggaggt cttgggctcc gtataggaaa gctccacaac cgcagctatt tttgtgttgt
     3901 acttttcggg cttttcacct ttgacatctt cgctgaggaa caggccgcct tcccaggcga
     3961 tcgtgaccgg accgacgagg agcgaaccct ccgtgccggc gaagtggtag cgggtgaagt
     4021 cgaagtcgat ggatgtgact tccacttcat cagggatata ctgggtggga tccaaggggt
     4081 ggggtttcat cttcagcttc gcaccgatgt cgaagcccat ctgcgggtag aagctggaga
     4141 agtacatgaa gccgagatcc agatcaccga tgccgaagcg atagcggcct ccgcctccga
     4201 gggctttcca gaatgtgggg tgttggtaga ttccgacccg gatattgtcc gagtcgattg
     4261 gaatctccat ccctccatag ctgaaggagg tacccatgag attgtcggta tccatcggtg
     4321 catagcggtc ggatgcttcc atgtcgatcg gccggtagaa gggcttgatg atgagatcga
     4381 gggatgagcg cttgaagtag gcgttgtaga cgatcatgaa ctcgcttttc ttcatcgctt
     4441 cgggattgtc cagaatccca tgggtgagat tctgggagtt gaggatgtcc acggcatgga
     4501 gggtcgtgct cgtcccccat gggtgggtga acattcccac cttgaggttt gtcgggccga
     4561 agaagaaggt gaccgccaag gtgcggacat caagggggtc gcgaaggatc ggactgaagg
     4621 agaaatcgac ctcggtccgg atcttgtccc ggttctcttc aaacgtcacc cccagcgaga
     4681 tgccgtagtc gaacggctca tcctcatcca gagggtagta ggtgccgaac ccctcgacaa
     4741 agagggacgt gagttctttc ggctttgccg gttcggtgcc gacttgccct tcttcatcat
     4801 ccaaggtcgc aaagaagtca aatgcgaaga gcggggaaac aaaagcgata agcaagatca
     4861 gtacaagtag tttcttcatc ggattacagc ctccctgttt ctaagaaacg aactgtgaaa
     4921 tacgcggccg gaatggggac atcatagcgg acctgaacca tctcgaggcg ggttgcatgc
     4981 cccgtcgcga tcgtcttcat ttcgaccatc ttggtaatcg ggcgggttcc gaccgtgccg
     5041 gtctccaacg tcgtgagggt tttcaacaag gttttttcat ccggcgcata gaactcgacc
     5101 ttcaggggaa ggaacgtctc cttgtccacg gtcgcaatat tctttccata gctcttgacg
     5161 gtcttgggaa tcgactcgac gacatacgtt gttgcagttt cttcgatcag gcggtgggtc
     5221 gcctcatcgg catcatacgt tgtcgatgcc atgtcggagt aggagaaatc gctgcccatg
     5281 aacgatcctc cctcttccgt ggcgctgatc ttccgtaccc gcttgagcgc cggaagatag
     5341 atccactgtt cggtagcgtc gtttccttca acggagagaa agcgggtgtt tttaacgctt
     5401 tcgggagaga ggaagacggt gatgatcgag gtcttgtcgt caattgtctg gctgagggtc
     5461 tggatccgcc gctcccgctc ggtgccgttg gcctcgatga gagtcagctt caggtccatc
     5521 gccgcggtgt cggccttctg ggtttcaatg accttgtcca tgatcagggc ggcggacgga
     5581 gcagcgatga tcaaggcggg aataagaata agaagcaaca tgataaacac acgtttcatg
     5641 atttggtcct ccttgtgaac atgagcttgg gataatgaac ggctatcgca ggaagcaaca
     5701 gcagcgcgct ggctgcagct gtcgccattg cgatgcaaaa cagcactcct aaatttgcaa
     5761 tcggcatgaa gcggctgaac aacaggccga tgaaaccaag ggcgacggat gcggcattgg
     5821 caagaatcgc cttgccggtc gtctgcatga tccgatccat gggcatatcg ttgcggacga
     5881 tcgcactgag gaggtggatc gcatagtcga ctccgattcc gatgcacagc gccgcaagca
     5941 agctggtgac gatatccagc ttgatcgaga aggcaagcat cgaaacgaag accgccaaga
     6001 gggcgaagag gcaggggatc agggcaaggg tagcgagttt cggggatttg agggtgaacc
     6061 acaccaagaa ccacacggcg atcagggagc tgaacagact gacgatctgg cttcgggtga
     6121 ccaaatccgc cagagcgagg gccaccgctt caccgccgcc gacttcgacc ttccaatcct
     6181 cgggaaagag ggtggaaatg aggtttgtta tggttcgaag ccggttggtg tccgaactct
     6241 tcagcaccag cgtcaatgca gtggcatcgg gttccaaggg atcattgatg aacatatcga
     6301 ggttgcccga gtagagcagc aggtattggg cgatgaggtt cgccagctcc gattcatcgg
     6361 caaggccgta acgcgccgga tcgccgggaa tctcatattc accttgccct ccggcgcttg
     6421 caaccacctc ttgtgcttcg accggctctt cctcgaaacc cagaaagaag tcatcgtcaa
     6481 aggcgaagaa gtcaaagacc acctcttctt catcaacggt gtgaacgatt tcatcctcac
     6541 cgggaccgag aagctggttc atccgcttca cgaacggaat gatcgattgc actcctccca
     6601 ccaccggttc agcttcaaga cgccggatcg ccgcttcaat cgttttgagg gtggagggat
     6661 cgagaatcga gatgtcctcg cccggctcga tcgtcacggt catcgagaag cttccctgca
     6721 ttcgttcgtt aaagcgattg gagtctttga ccagggatga agaggggcgg aagaaatcga
     6781 gcatgttcgt tccgctctcc acgaagtagt aggagagcgg aaggaagatc accaagatga
     6841 tcatggagac ccacaggagc gctttgccgt gatggtttgc caccaccatc gctgcagtca
     6901 tcaaccgcat atccttgttt tgttgcttct tccgctcttt tccgttcggt tgtttgtaga
     6961 caacccgaat aagagcaggc agaaggacaa gggaactgaa ggcgcagatg acgacaccga
     7021 tgaagctgag aagtccgaag attctaaagg gtcccaaggg agaggagagc tgggccaaaa
     7081 agccgaaggc ggttgtagcc gcagcggcga tgatcgggta ggtgttccgc ttcacgacga
     7141 ctcggagcac ctcttcgatc gttccggaat cctgattttg gaagaagtgg ctgaagatgt
     7201 ggatcgtata ggcgctgccc acgatgagga gcaataccgg aacgagcatc gtcgccatcg
     7261 tgaaggtgat cttggctatc gccatgattc cgagcgcgac ggaggaggag aagatcaggg
     7321 gaacgagcga aagcagcgtc gctttgaagg agcgaaggaa gtagtacagg atgaggatga
     7381 tcagggttgc cacgatcggg actaaaatcc ccaagtcact gagtaaactc tcactaatcg
     7441 cctcagtgac tacggggaga cctaagatcg aggtttcaac aaccccttgg taaggagcga
     7501 cgacttccct gaggtcatga agaagctgtt ttgaatcgta gtccaaggtt gtttgaataa
     7561 ggacggaagc aacagtatgg tcggccgata gaaatgtccc ttcataaaag gaaggccact
     7621 cttcaatccg attcttcaac gcctcaaagt cgccgtcgta cagcgatacc gcttcaaccc
     7681 catcaaagga tggttccagg tgttgtacat ttgcaatcga gttggcggtg gagactccgt
     7741 ccaatttctc gatctcctcg gtgatcgtct tcaagatctc catgtacttc ggttgaagga
     7801 tcgtatcctc tgcatagata ctcacaacca caggatccaa cgagccaaag cgttcttcaa
     7861 ttcgatcatt gatttgcaca acatctgcat ttgtcggaat gaaggcatcg gtagaactgt
     7921 ccacatgaag ccagcctatg ccgagaaggc ccagaacggt caggacgaat gaaagtgaaa
     7981 taatgatcca atcgagggtg cgtcgttttt ccatgaccat agcctcgctc tttaaagcag
     8041 atccaacaat cgccttcaga cggtatttgc atcacccgtc ggggtgattt ggatatcacc
     8101 tcatggaagg tgaggaaaga atcgaaaccc tgttccgagt ttgaaatgac cttcagcgat
     8161 atcgttgcca aataatgatt gccaggttgg gacatttgct ttacaaattc cgtccgatca
     8221 cagatgattg acctatgaac tataccaaaa ttgtagccac attagggccc agtagtgatg
     8281 agtatcagat catcaaagcg atccttcaag cgggatgnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnntgc cggatggtgg aagagggcac gatactgccc cgcaagggag
     8461 tgaaccttcc cggggtgccc cttgttcatt tgtcctcctt cacggaaaag gacaaagccg
     8521 atttggcgtt cggttttaag aacaagatcg actatgtcgc tctttccttt gtccgaagcg
     8581 gtgacgacgt ggcgttgttg aagaaataca tgacggagac gttcggccat gcaattccca
     8641 tcatcagcaa gatcgagaaa ccggaagcgg tcgataatat tcaccaaatc atggacgaaa
     8701 gcagcgcgat catgatcgcc cggggggacc tgggtgtcga agttcccgct gaagaggttc
     8761 ccctgatcca aaaatcaatc attcagatgg ggatcaatcg aaacctcccg gtgattaccg
     8821 ctacgcagat gttggaatcg atgatttatc gcccccgacc gacccgtgcc gagaccaatg
     8881 acgtggcgaa cgccgtattg gatggaagca gcgcggtgat gctcagcggc gagaccgcag
     8941 caggggggta tccgcttcag gcggtccgca cgatggcgaa tatcacttcg atgatcgaac
     9001 agagcgaagt attcaaacga gaggtgatga accagatctt agggatggat atgctgcgca
     9061 agcagaccgt aacggaagcg gtggggctgg ccacccgaga tctggcgatc agcgtgaagg
     9121 catcctacat cacctgtttc acccactcag gctcgaccgc tcgcctgatc gccaagttcc
     9181 gtccttcggt gccgattctt gcattcagcc cgaataaatc ggttgttcag tacctctgtc
     9241 tgagctgggg ggtgaccccg attctcatcg atgaattgga aagcgtcgat gaactgcttc
     9301 tcttcgcccc ggaatacttg aagaaaaacg gtcttgtgaa gaccggagac gccgtggtca
     9361 tcaccgccgg agtcccggtg ggctccagcg gcaaaaccaa tatgatcaaa gtggttgaga
     9421 tcgaatgact acggtcggaa cgtcagagca gtgctgaagc tcaccgctct accggcatga
     9481 tcgaagagat cgagggctcg aacatcgaaa gatattgtcg ttgaggggaa gagcttgaaa
     9541 gcgggaacac ggaggcctac gttgaccaat ccccggttct gggcatttcc acccgagggg
     9601 ctgaggctgt aggagatatt cccgaagtcg agaagaagag ccaaatggtt cttcagcttg
     9661 ctggggaaga gcatcgtctc aaggccgacg gcgaagtcga tgttcgaaag agcctgagtc
     9721 ccctgcttcc aggagtaccc gaccatgatc gatgacttgg tcggcaggtc catcaaggtg
     9781 tggcggaacg tggatgcaag gtatccctga acaccaaacg tattggaatc gcccacttca
     9841 atcagttgga ggttggctcc tacggcaaat gatgtgcctc ttttttcggc aaaacgccat
     9901 ttcgccgaaa gaagcaaatc ggtcgcggtt tttgtatcaa cagcgaacga cacttcaaca
     9961 tctatgggaa aggaaaggta gatgaagggg acgtgggcaa gacctttgtc gccgtagatc
    10021 attgaatagc cggtggcgat tgcaagcttg ggtccgcttt cttccacgtt ggcgctgggg
    10081 gcgatgatat agccgctggt cccgtttaac gtgggctcgg cgagtaacag agcggttccc
    10141 aacaagagga tacctacgat gagaagggtt cgtttcataa cgggctcctt ggttcttcgg
    10201 ttgcctttct taattcaggc tgaccttttt cgttgattcg tttcgtgatg tatttttctt
    10261 tctccaattg agcgagaagg gctcccaacg atttatattt cgagcgctca atcattcggc
    10321 tcttatccat ctggtgaaat ttggttccta caaaactggc aagctgccaa ctgtcggggt
    10381 caaaggagtc gatgaccttt ttggccagag ccttgacctg atcattcatc ttctcctgcg
    10441 gcgtcggttc gacggagacc tttgtttcct tcttggctcc ctttgtcttg gtcgtgtagg
    10501 actccaggat gagaatctcg tcgcagctgt tggaaaacac cggcttgtcg ctgacctcct
    10561 ccttggtgac gaggaacact tccttgccat actttcgaag catctccatg atgacggtga
    10621 aatcgctgtc actggtaata aatacatagc gatcaatcac cggcatattg atgatgatgg
    10681 tttcaagagc ggccaaggag atgatcaggt ccgcccgatt cttgtacttc gatgtgatcg
    10741 agggggtatc ccggatctcg aagttgtact cggtgagttg cttcttcatc tttcggatcg
    10801 ccgtggagtt ccccgccgcc atcttgatga cgaagacatt ctcctcgtcg ctgtccacgt
    10861 tatgccgcaa ggagaggtcg tcaagcaagc tctttaaatc aagggttgat ggaatgtttt
    10921 ccaagtcgat gtaaatcgcg ttgtttttcc gtttcatagg agaactatac tccatcagca
    10981 gcaatcctaa aaggggacag gtccatctac gcggatttct tcctttgtaa tcgggtgggt
    11041 aaactccaag ctcttggcgt gaagataaag gcgactcttc atccccgagc cgtaaagacg
    11101 atcaccgaga atcggaaggc caagaccttt tttgctgtgg atccgcagct ggtgggtccg
    11161 tccggtatac ggggtgaact cgacccggct gaggtgtcct tccggatgct cctccacccg
    11221 aatccgtttc acatgggttc ttgcttcttt accaccctca tgatcgacga cttggtaagg
    11281 ccgattggtg atgtcgggtc gaagcggaag gcttatttcg gtttcctctt ctttaaggat
    11341 tccttcaagc agcgcctcat aggttcgtct tacccgttgg gtccgaaaga ggtgatgcat
    11401 cccgctgagc gcttccttgg taagtgcaac gacgatgagg ccggaagtgt ccatatccag
    11461 gcggtgggct gcacattgaa ccggcgccga cgggatgagt tttcgtactc tgctttcaat
    11521 cgaatcactt tcaaccgttc ccgggatcga gcggaggtgg ggaggcttgt cgacgatgag
    11581 aagatgcttg tcccggtaca cgggataaag tccgagcatc cgtttaagaa tcggagcaca
    11641 gcgttcctca cagggctgat aaaatcccaa gtgagagcgc gtgccgtcgc cgtagaagaa
    11701 ctcggtcatt gacgtgggaa cgatatggtt gcccaacgcg taggcaagga gcttgattgc
    11761 tgcgcagtca ccgctgccgg tcggaatcgg tcgattacca aaacactcaa acagggagac
    11821 ggtactaccg tcaattgtcg gaaaccgata gagaccgtag tagtggttca gcgcttgggc
    11881 ggagtattca cttttgttaa agggggtgtt gtgcggattt tttatcagtg catcgtaggt
    11941 atcaagatgg gtttggaaat ccgtatcggt gatcacatgg ggtacatagc gagcaagatt
    12001 cctttgttgt ccgctgatcg ccttcagcag aacctcgttc ccatatcgat cagttccgag
    12061 caggactccg atcatcaccc cgccatgctc cttcaccgga agcgttttgc ccgagtcgaa
    12121 atcaagaaag ccggttttcg tgatgaggtg ggcaagatac cgggcatgaa ggcgagcagg
    12181 ctcttcatcg aatatattaa tcatcgcttc gtttcatcca atcggcggtg aagtaataga
    12241 tgatgaagat gatcgaactg atcgcccagg tgagcgggtg agcccagaaa acaatacgaa
    12301 tatcgaagaa gagggcgaga cccaccttga tgaacatgat ccggagaaca caccagatgg
    12361 agagcatgac ggtcatcgga acgatcgatt tgcctgctcc gcggaagagt cccgtcatcg
    12421 tgtgactgag ggcaagagcc cagaagaacc atccggcgat cagacccttt tgcacaccga
    12481 ggcggatgat ctcgctgtcg cgggtgaaga tcgtcatcac ctgaccgccg aagatcatca
    12541 gtgcgaaccc caccagttgg gcgagagcca tcgcgaagaa tatgccgaaa cgggctgctt
    12601 tcctcacccg atcttgtttt ccggcgccga ggttttgccc ggtgaacgtc gtcagggcga
    12661 gggcgaatgc ggtgatgggg atgaacgcaa agccctggat tcggttgtaa gagccgtggg
    12721 cggccatcgc cgccggaccg aagaggttga tcgatgactg gacgatcacg tttgcgaagc
    12781 tggtaacgga gttttgaatt cccgacggaa caccgagcct gagggtcttc cccaaatacc
    12841 cgcggtagat cctgatctct ttgatccgga cttgaatgac ctcttttgtt cgaatgagca
    12901 gtcggaaagc gaggatcgcg ctgacgcttt gggcaatgat cgtggcatag gcggttcctt
    12961 cgattcccat cccgaaggag atgacgaaga ccaggtccag cacgatgttc aggatggtgg
    13021 aaataatcag aaaatacagg gggtttttcg aatccccgac ggcttgaaga atcccgctgg
    13081 cggtattgta cagaacgagc gatgaaacac ccaaaaagaa gatctgaatg taggtttgag
    13141 catcggtgaa gacctccgga ggggtcttca tccagaggag gatcgccggg gtgaacgtat
    13201 agccgatgat tgtcagcagg accgaactga tgatgccgag ggcgacagtg gtatgaacgg
    13261 cttttgttac agaatcgtaa tcaccctgac cgaacgcgga ggaaatgacg accccggaac
    13321 cgacgaagac gccttgaaag agtccgacga tcaacattgt cagcgatcca accgagctga
    13381 ccgcggcgag cgcctgagtg gagacgaagt ttccgactac cacagcgtcg gtcgtatgat
    13441 agagctgttg aagaagccca ccgaggaaga tgggaaaagc aaagaatata agatgttttc
    13501 tgatcgagcc ggcggtcatc aatcgcgtct gggtagccat aatagtcact gtagacatct
    13561 ttcatgaatg acgcaactac gcattcttaa ttcatgtcca ttatgggcgt atcaaagatc
    13621 cggccgttca gcggggtgct gtcaatcttc agaaaacggg ggccccggct ctagcttctt
    13681 cgcatgtatg tgggtatctt aaatcaagat acggaggggt tcgtatgaaa ctagagttaa
    13741 ttcgtcatgt ccatgtgtct gaagagtact tcaccggagc agggaagtat catcacggca
    13801 atcaagaggc aatagaagta cagatgaagg ctgtcgccaa ccaagggaag gcgaagtaat
    13861 atacctttgt tccacgtagc agagtgttcg tgtctatcat gcaggcttga tagaacccaa
    13921 tggtgaagga gagacacgat ggcatttcca tggggtaata aagaactggt gtctccgcca
    13981 ggtaaaacaa tccgagacct cattgaagat cgggggatga cccaaaaaga gtttgcgact
    14041 cggatggatc tttcggagaa acacgtcagt aagttgatca gtggagaagt gctgctcacc
    14101 accgatgtga ttttgagaat gatttcagtt attggaaagg atgttctctt ttggtcaaat
    14161 ctggaagcgt tgtatcgggc cggcgtagtc agagaaacgc tgaaagacca ggtagccctc
    14221 gatgagcgga ttcttccccg tattcccctc aaggagctga gcgaccttgg ctggatcgac
    14281 cttgatgatc gtccggaagc gcaggtggag cttgcccgtc aatactttga ggtttgtacc
    14341 ctccaaatca ttgaaagcga gtcgatgaga tcatacactt cctacggggc gtacatcgac
    14401 cgtgaaatgg actatacgct tcttgcttgg attcagcaag cgaagttccg tgcccgcagc
    14461 atgggcgtcc gaccgttgga tcatcggaag ctggaacgga atatgatgaa agtccgctcg
    14521 ttatctctcc tttctccgaa agagtttctt cccaagcttc agggacttct ggcgcactgc
    14581 ggagtagcgt tcgtggtgct tcccgccatg aaggagcttc gccttgaggc gatcaccttc
    14641 tttgccgagg ggcgggcgac gatcgcgttc accgccgagg ggaagaactc ggaaacattc
    14701 tggatctctc tgttccggga actggctcat attctcctcc gacatgtggg gccgcgcata
    14761 gtatcctcaa aagatgccat atccgaggct gagtcccttg gctttgaact gttgcttcct
    14821 tccgaccgat ggaagacctt ccttgcccgc aagcgatttg acgaaaaagc gattctcacc
    14881 ttcgcccaag aagaaaagat ccacccttcg ctcgttgcca atcggctcgg gaattcgaag
    14941 aaggagcttg cccatctcac tgttgcgtat gagctgctcg aagcgatatg aacaagccgg
    15001 agcatccggc aaaatagaaa tcctgcaaaa tccgaagagc gaggcggggc tatcatcatt
    15061 acggctcatc aagctccatc gccggaatcc tttatcaaag cggaaacctg caaccgcacc
    15121 aacgagacgg tattgttcgg cagatcatcg acagggaacc acctcagccg ggaaaactcc
    15181 tcatgacaaa caagattccg cttctcaggc atgggatatg cgaagactct gaagtggtag
    15241 aaagggatat gggttcgcca cagaagagca aggtcatcac catcgtcgat tgacagccgt
    15301 atctcttccc atgtctcccg aatggcggtc gctcgatagt cgggcgtcgt tccttcttca
    15361 aacccgtcct tccgctcatc ccactcgccg ccggggatcg accacatgtt cgcttgagga
    15421 ttgtactttc tcaatccaag aagaacctcc gtcttcccgt gagcgtcttt ccgccatgca
    15481 aggatgccgg ccccatgata ggggagccgt ttcattgctc ttccaagatg tttgattact
    15541 gaatcgttga atgacatgat tctatgtact taccttccag ccacccgcga cgacttcggc
    15601 gcttgaacgg tacgttccga gcagttcatc ggtgtcttcg tcgaacaccc gccaatttct
    15661 ggcaagcgca tcatcgatcg tgatataccc ttttctgcca tcgagacggt ccaggggaca
    15721 ccccttttta accccaccgg ggctgaaggc gaaggcgacg atatcgaccc gctcca
//
LOCUS       DAOSLI010000042        14828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_4167_length_14828_cov_31.253300, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000042 DAOSLI010000000
VERSION     DAOSLI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 14828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14828
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4167_length_14828_cov_31.253300"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1171
                     /locus_tag="PLF67_06765"
     CDS             95..1171
                     /locus_tag="PLF67_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE89750.1"
                     /translation="MKKLFVLLCVLLVLSFSLFAQGTKDAGKATLRVGMVTDAGTIDD
                     KSFNQGTWEGILRAQKDFSLDVKYLKPAGTTEADYLKEIGNLVDAGYTMIICPGFKFE
                     TALFEAQDKYPNTKFVLLDGEPHSADYSVFRIEKNVVGLYWAEHQSGFLAGLAAALQI
                     KNGSFGFIGGMEIPAVQKFNWGFQQGVRYANANYKTNIRMDQVNNLYQGSFDNISAGQ
                     QIAAAMYDRGVDVIFAAAGGVGVGVINEAKNRGSSGQKVWVIGVDVDQYAEGLMPNGK
                     SIILTSAMKYLDRASYDMIVDELNGRFPGGEILRLNATNDGVGIPAENPNLDKSVTDE
                     VAKVFAKLKSGEVTVADKSDGLFK"
     gene            1239..2762
                     /locus_tag="PLF67_06770"
     CDS             1239..2762
                     /locus_tag="PLF67_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89751.1"
                     /translation="MNHVIEMLNIRKEFPGIVANDNITLQVREGEIHAILGENGAGKS
                     TLMSILFGLYQADEGTIKVRGKEVQIRDPNDANDLGIGMVHQHFQLVHNFTVTENIIL
                     GKEGGFILHRKSASSRIADLSARYGLNIDPDMVIEDITVGMQQRVEILKMLYRDAEIL
                     IFDEPTAVLTPQEIDELMQIMRNLVKEGKSVILITHKLAEIKAVADRCTIIRRGKLID
                     VVDVAKTPVSQMASMMVGRPVSFKVEKKPAQVGRVILDIHDLTVMGNKKVPAVKDLSL
                     FVRSGEIVGIAGVDGNGQSELIEAITGLRPIDSGVVSLDGNDITNAPIRTRNEIGMGH
                     IPEDRQRRGLVLSSAVYNNVAIKEYYHPPFSKRGLLNIEYLQEYARTVVDQFDVRSGE
                     GIFSNAGKLSGGNQQKLIVGREMVASPQLLIAVQPTRGLDVGAIEYIHSQLVAHRDKG
                     HAVLLVSFELDEIFNLSDRIAVMHSGRIMDVVLPSETDEATVGLMMTGIEAKGAGHE"
     gene            2755..3843
                     /locus_tag="PLF67_06775"
     CDS             2755..3843
                     /locus_tag="PLF67_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89752.1"
                     /translation="MSKIKRKESLGSNAFLMAVSAVILGLVAGAILMAAIGSNPFSGF
                     YYLFRGGLMNIERIGNTMATATTLMLVGLSVAFAFKTGLFNIGAAGQMLVGGLCATIV
                     AHKVFLPRPLFLVVLIASGLLGGAIWGVIPGLLKARFNVHEVVATIMMNWIAYWSIYY
                     FVPAYLKGPSLETESASIALTQSLRTPWLTKLFNGSYMNLGFFIAIICVILVKFILDK
                     TTLGFGLKTVGFNRHCAEYAGIKVNSNVVISMMIAGGLAGLAGLTFYTGYSRNMQIGV
                     LPAQGFDGIAVALLGASNPIGVFFSSLFFGILQSGKGFMNAMTSVPPEIADTIIATII
                     YFTATSVLFGRIWESIFKRRRKSAKEEQ"
     gene            3845..4777
                     /locus_tag="PLF67_06780"
     CDS             3845..4777
                     /locus_tag="PLF67_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002669777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89753.1"
                     /translation="MWSTLSSIFPYAIGYTMPMLMAALGGLYSERSGVTNLGLEGLMV
                     IGYFFSALAVKLTEATLGANALGLGLLVGTVAGALFSLLHAFASINLKADQVISGTAI
                     NMIAGALTVYLARTITGSGNVRIMMGIIRKNIPVLSEIPIVGPLFFTQTYWTTWICLL
                     VWLVSYFLLSKTSFGLRLRACGEHPSAVASAGVNVHLMRYIGVIISGALSGFGGSVIL
                     ITYSGEFNGTVAGLGFLSIAALIFGQWKVLGILGATFFFGIATTIANVSQVIPSLMIV
                     PPVYFKIFPYVATLLALVAFSKRSAAPLASGQPY"
     gene            4791..5621
                     /locus_tag="PLF67_06785"
     CDS             4791..5621
                     /locus_tag="PLF67_06785"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOE89754.1"
                     /translation="MDNLMKKMQEAAMFITSRIGQIPVDIGMVLGSGLGDLADDLANS
                     IAISYKDIPHFPVSTVFGHKGRLVAGDLEGKRVLCMQGRFHYYEGYGMDQVVFPIQVM
                     QALGIRHLLVTNAAGGVNTSFSVGDLMLITDHIKLIADNPLRGPNPDQLGERFFDMTE
                     AYDKKLIELAKAQAEELGIAVREGVYMFFSGPAYETPAEIRAARVLGADAVGMSTVPE
                     ALVASHMNMRTLGISCITNMAAGVLNQRLSHQEVMETGERVKEDFTRLVHAIVRHWPV
                     "
     gene            5618..6031
                     /locus_tag="PLF67_06790"
     CDS             5618..6031
                     /locus_tag="PLF67_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE89755.1"
                     /translation="MNLKEYAKTFDLLPLEGEGGFFRRVHTFKDGSVIYYALDEEDFS
                     YTHRLVTEEVWFHLDGFPVTIVLIDPEGNEEEHLLDKSNPILVVPGLWWQSARPKEGA
                     ALCATVMSPAWKEEEFTLAEEAFVNLHPKTRRLVQ"
     gene            6028..6726
                     /locus_tag="PLF67_06795"
     CDS             6028..6726
                     /locus_tag="PLF67_06795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE89756.1"
                     /translation="MKTMLISGGTSTIGEAAVRYFTQKGWHVYFGYNTAREKAEALET
                     ELGPSAAALHLNVQDKDSIKTLASSIDELDLLINNSGIFSSFLTEALEDAEVGRIFDV
                     NVTGLFRLAKAVLPKLKEGASIINIASVNALMPTFGSTTHYDASKGAVIAYTRSLAAE
                     LAPKIRVNALAPGLVAAPYLDEKNEIRKIFEKRSVLKRMVTSEEVAHALLFMAENTAL
                     TGAVIPLDCGYTIG"
     gene            6729..7454
                     /locus_tag="PLF67_06800"
     CDS             6729..7454
                     /locus_tag="PLF67_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE89757.1"
                     /translation="MKILCISDETDPLIYSEHITTRYGDVDLVLSAGDLPLKYYEFII
                     SMLNKPLYFVFGNHNQEYLHQYLSNNQSPSEAFHQQVVGLQPFGGEFVDGRVRYDKRN
                     KLIIAGLGGSMRYNNGDHQFTDFQMFRRIVKMIPRMIFNRIVRGRYVDVLLTHAPPLG
                     LGDGIDLPHKGFSSLLVFMKWFKPAYLLHGHVHLVDFNANRVHLFRQTKVINIFRNYL
                     LEDDQLGGETDVQDGWRPARPDK"
     gene            7414..8331
                     /locus_tag="PLF67_06805"
     CDS             7414..8331
                     /locus_tag="PLF67_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOE89758.1"
                     /translation="MSKMVGDLLAQTNEDFEKAKSRGRIQSILSSLVRKNSDLLSLYA
                     VTDLIKPKGETYRGMQTIPVKNIIGSEGRYQDFSLAFYPKKELLRARWRSIDLATKKY
                     VILPPISVFKLGEWYFVRDGNHRVSVAKTQGVEFIDAEVVELDSEIPLEPGLTMKQLR
                     RRVVYYERKRFLEQYDVDCLPMDRIMFSNAGSYAELVNHILVHKYFLNEDSDRELTFK
                     EGAISWYTTVYEPIIAEVRKQGVLTSFPGNTEGDLYMWIVRHWDNLKHMSGSHEVSIQ
                     SATSDYKARYGKTLVGRWLHKLKEFLGKD"
     gene            complement(8328..8921)
                     /locus_tag="PLF67_06810"
     CDS             complement(8328..8921)
                     /locus_tag="PLF67_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOE89759.1"
                     /translation="MKRCSIIIHSVSGNCFIIGNYLKELMAERNVDARLYRVDDPDLH
                     IWANTQETTNEFYEDICELPLVGDSTLHKSDMIVLGGPTRFGNISAEMKTFLDSTLPL
                     SKDKSLETKFFACFTSCSNSTCEGAHTLNSLIYWAQSMGMLHIPFGVHYELEFSDQPV
                     SGIVHLAGKEDSIRPGDRLGHELEVYADILAAYIQED"
     gene            complement(8974..9753)
                     /locus_tag="PLF67_06815"
     CDS             complement(8974..9753)
                     /locus_tag="PLF67_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE89760.1"
                     /translation="MRTLIELRHATVQRLGQRILNDVSLTVKRNEHVAIIGPNGAGKS
                     TLVQTLSMEVHPLHTPDFKRILFGKSRWQILELRKHLGIVSASLQHVCTSTYKAWEIV
                     VSGFFSSIGLDFHHLVEESHLKRAKEVMNTYGILPLWEKQMNRLSSGEARRVLLARAA
                     VHEPEVMLLDEAGASLDFPSRHTYRKSLEQLDREGKTIILATHELSEIIPAIGRVVVM
                     KNGAIVADGPKEEIFNEALLREVYEAPIYLDYRDGLYSVWS"
     gene            complement(9772..10524)
                     /locus_tag="PLF67_06820"
     CDS             complement(9772..10524)
                     /locus_tag="PLF67_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE89761.1"
                     /translation="MSDLPQTKSTVIAQTIERWIKDGTLECGSYLPSQVELAEQFRTS
                     SRPIREALKILEARGLVVLSQGRRAQIRSNNLDHYVMSVSSSILNSDISHSKLLKNLM
                     QVRLTVASSAARNFSRLEGRKRYLTQLWAASRQMEVSLPGIYDREAQATQSFLDAEMD
                     FHHTLVMANNNQILSAIYENLSPLLDQAMRSIRFSAGQLDKRAKNYAYLCEALENGQT
                     DLAVALVMVILSTLEDKVLDSYPEEQRRASYA"
     gene            complement(10701..11429)
                     /locus_tag="PLF67_06825"
     CDS             complement(10701..11429)
                     /locus_tag="PLF67_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE89762.1"
                     /translation="MAKVVETKSSVIAAQLEEDILAKKYPAGGNLPSQQELAEMFGAS
                     SRSIREAFKSLEAKGLIKVQQGRRAEVQTNSLERFVETLTSSMISTSAMDKKMIRDLM
                     QVRTTIEVSASRELSRDPNRTLIVRSLSRACTRMEQILPSLENSKDEEALAEFKDVEL
                     EFHATLIKSNDNAILTHIYDYLAPQLYSAMERLTETFKEQQKKVNEYRYLVDALENGQ
                     TDLAVALTLVNLTNISEKFEALEF"
     gene            12070..12615
                     /locus_tag="PLF67_06830"
     CDS             12070..12615
                     /locus_tag="PLF67_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89763.1"
                     /translation="MKKLIALLMILAVVLTGVFAEPGDEQSQVKVYLKGTIGAFFRHG
                     VKENDALVYEKTYQSESVLSATGTNFQYGYQSNQDILGILYVDYTDFSDGNNHSIQIG
                     TLKIGGTAMTADNYTQGSGFKVFTNLTGTNMSIQEKAIQVIAAQVKAGNDPKGTAISH
                     IVGEAHKGTYTATLTFQVKAN"
     gene            12717..13268
                     /locus_tag="PLF67_06835"
     CDS             12717..13268
                     /locus_tag="PLF67_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89764.1"
                     /translation="MKRLLLLSITLFLLLPLAGRTITAGLGEPVAVSLKGTIDSKELT
                     ISVRGEGMDEELNEYDVLMFDFPITDHDKRSWVKEVPLHFTYSSNKNLPSKAHLEFVV
                     GEFVGDSGFKLKPELKIARSSNKIDLDSDPRRILLETTFESGRQEDTPIVTVVVVFTK
                     AEEELFPVGNYTGVFSINYLGIN"
     gene            complement(13269..14690)
                     /locus_tag="PLF67_06840"
     CDS             complement(13269..14690)
                     /locus_tag="PLF67_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89765.1"
                     /translation="MYLNKLFERSTGRTLLLFQETKRVKGKNRTRTVERIGYLDELEK
                     IYDDPIAHFKEEAKRRSDRRKLNVEVSLDEHHQFDAESREVKAPGLSLGMLPLSQIYH
                     ELEIGSFWDNRRRYTKARYNHNAIFQALVFGGILFPESQLSTWRSRLGLLSNMDFTDE
                     DVRHALPFFEAHKTALQKHLHRKVGTLYGRDPSVLYRARVPNGRAGEEHHIQMGLLTD
                     DNGLPIAYGLFGNDGSTLARMVKEVDEQLEIQKVVHVSDKGMISANDKEEILANKGGY
                     IFGTPLSKTDADAKAYIPDETGYKHPNASERMTVNERQIVFREENGYHVIHTNVIGSE
                     GDPSSPLFRLEKPATALDIIDMYRGLWRIEERFLGSNQEKESIEAHLLSRFVSLLILK
                     ILELKTGKSIEFEKMVDDLRRLQVVEETDDVYLLLEPTNIIKAIGDAMDLDLVKVRYT
                     ARELKNLVAKTRKQGMVPDSARG"
BASE COUNT         3474 a         3804 c         4006 g         3544 t
ORIGIN      
        1 ggtcttctcc ttctttaatt tgacatctga catacaccct tgtaaaatga atctacgtgt
       61 aaagatctta tgagatccaa taaggagtag aagcatgaaa aaattgtttg tattgctctg
      121 tgtcctcttg gtgttgagtt tctcgctctt tgcccaagga acaaaagatg cgggaaaagc
      181 caccctcaga gttggaatgg taaccgatgc cggaaccatt gatgacaaat ccttcaacca
      241 gggaacctgg gaaggaatcc ttcgcgccca aaaggacttc agtctggatg tgaagtacct
      301 caagccggcc ggaaccaccg aagccgatta cctcaaagag atcggcaacc ttgtcgatgc
      361 cgggtacacg atgatcattt gcccgggctt caagttcgag acagccctgt ttgaagcaca
      421 ggacaagtat ccgaatacga agtttgttct cctcgacggc gagccgcaca gcgccgacta
      481 ttccgtcttc cgcattgaga agaacgttgt gggtctctat tgggcggagc accaatccgg
      541 cttcctcgcc ggtctggctg cagcgctgca gattaaaaac ggcagcttcg ggttcatcgg
      601 cggaatggag attcccgccg ttcagaagtt caactggggc ttccagcagg gagtacgcta
      661 cgcaaacgcc aactacaaga ccaatatccg gatggatcag gtgaacaacc tttatcaggg
      721 atccttcgac aatatcagtg ccggtcagca gatcgccgcc gcaatgtacg accgcggtgt
      781 tgatgtgatc tttgcagccg ccggcggcgt cggtgtcggt gtcatcaatg aagccaaaaa
      841 ccgcggaagc agcggacaga aggtatgggt gatcggtgtc gacgtcgacc agtatgctga
      901 aggtctcatg cccaacggca agtcgattat cctcaccagc gcgatgaagt atctcgaccg
      961 ggcatcctac gacatgatcg tcgacgagct caacggccgg ttccccggcg gagagatcct
     1021 tcgcctcaat gcgacgaacg acggggtcgg aattccggcg gaaaacccca acctggacaa
     1081 gagcgtcacg gatgaagtgg ccaaggtctt cgccaagctg aagagcggcg aggtcacggt
     1141 cgccgacaag agcgacggcc tgttcaagta attgaaggta tctttgtgta tgggccacct
     1201 ttcctggggt ggcccttctc ctcaatccgg gggttgtaat gaaccatgta attgaaatgc
     1261 tgaatattcg caaggagttt cctggaatcg tggcgaatga caatatcacg ctccaagtcc
     1321 gcgaagggga aatccatgcg attctgggtg agaacggagc gggaaaatca acgttgatga
     1381 gcatcctttt cgggctctac caagccgatg aagggacgat caaggttcgc ggaaaggagg
     1441 ttcagatcag ggaccccaac gatgcaaacg acctgggaat cggcatggtg caccagcact
     1501 tccagttggt tcacaacttc accgtcactg aaaacatcat cctcgggaaa gagggcgggt
     1561 ttatcctcca ccgaaaatcc gcatccagtc ggatcgccga cctttccgcc cggtatggcc
     1621 tgaatatcga tccggacatg gtcatcgagg acatcaccgt cgggatgcaa cagcgggtgg
     1681 agatcttgaa aatgctctat cgggatgcgg agattcttat ttttgacgag ccgacggcgg
     1741 tgctcactcc gcaggagatc gatgagctga tgcagatcat gcgcaacctt gtcaaagaag
     1801 gaaagagcgt gatcctcatc acccataaat tggcggaaat caaggcggtc gccgaccgtt
     1861 gtacgatcat tcgacgagga aagctcatcg atgtggtcga tgtcgcaaag actcccgtgt
     1921 cgcagatggc ctcaatgatg gtcggccgac cggtgagttt caaagtagaa aaaaaaccgg
     1981 cccaagtcgg acgggtcatc ttggatatcc atgatctcac cgtcatggga aacaagaaag
     2041 taccggcggt gaaagatctt tccctgtttg tccgcagcgg agagattgtg ggaatcgccg
     2101 gggtggacgg gaacggccag agcgaactga tcgaagcgat cacggggcta cggccgatcg
     2161 acagcggggt ggtttccctc gacggcaatg atatcaccaa tgctccgatc cgaacgagaa
     2221 acgagatcgg aatggggcat attcccgagg accgccaacg gcgcggattg gtcctttcca
     2281 gcgccgtata taataatgta gcgattaaag agtattacca tcccccgttc tcgaagcggg
     2341 gattgctgaa tatcgagtac cttcaggagt atgcccggac ggtcgtcgat cagtttgacg
     2401 tccgcagcgg tgaaggaatc ttctccaatg ccggcaagct cagcggggga aaccagcaaa
     2461 aactcattgt cggacgggag atggtcgcaa gcccccagct gctcattgca gtgcaaccga
     2521 cccggggttt ggatgtcggg gcgattgaat atattcactc ccaactggtt gcccaccgtg
     2581 acaaaggcca cgccgtgctc cttgtttctt ttgaactgga tgagatcttc aatctttccg
     2641 atcgaatcgc ggtgatgcac tccggcagga tcatggatgt ggtcttaccg agcgaaaccg
     2701 atgaagcgac agtcggcttg atgatgacgg gaattgaagc gaaaggagcg gggcatgagt
     2761 aagataaaaa gaaaagaaag tcttggcagc aatgcgtttc tcatggcggt cagcgcggta
     2821 atcctcggct tggtcgcagg agcgatcctg atggcggcca tcggctccaa cccgttcagc
     2881 ggattctact atctcttccg aggcgggttg atgaacatcg agcggatcgg caatacgatg
     2941 gcgaccgcga cgaccctgat gctggtcggt ctttcggtgg cgtttgcgtt caagaccggt
     3001 ctgttcaata tcggagcagc cggtcagatg ctcgtcggcg gactctgcgc cacgatcgta
     3061 gcgcataagg tgttccttcc ccgtcccctc tttctcgtcg tcctgatcgc aagcggcctt
     3121 ctgggcggag cgatctgggg ggtgattccg ggtctcttga aagcccggtt caacgtgcac
     3181 gaggtcgtcg cgacgatcat gatgaactgg atcgcctact ggtcgatcta ctatttcgtt
     3241 cccgcgtatc tcaaaggacc ctctttggag actgagagcg cttcgatcgc gctcacgcaa
     3301 tctcttcgca ccccgtggct caccaagctc ttcaacggat catatatgaa cctcgggttc
     3361 ttcatcgcga tcatctgtgt gatcctggtc aagttcatcc tcgataaaac aactctgggc
     3421 ttcggcctca agacagtcgg attcaaccgt cactgcgccg aatatgccgg aatcaaagtc
     3481 aacagcaacg tggtcatctc gatgatgatc gccggcggtc ttgccggcct tgccggtctt
     3541 accttctata ccggttattc gcgcaatatg cagatcggcg tcctgccggc ccaaggcttt
     3601 gacgggatcg ccgtggcgct tctgggggca agcaacccga tcggggtatt tttcagttcc
     3661 ctcttcttcg ggatcctgca atcggggaaa gggtttatga atgcgatgac ctccgttccc
     3721 cccgagatcg ccgacacgat catcgcgacg atcatctact tcaccgctac cagcgtcctc
     3781 ttcggtcgga tctgggagtc gattttcaaa cgccgaagaa aaagtgcaaa ggaggaacag
     3841 tagtatgtgg agcaccttat cgagcatctt cccctatgca atcggctata cgatgcccat
     3901 gctgatggcc gctttgggcg gactgtacag cgagcgaagc ggtgtgacca acctgggtct
     3961 ggaagggttg atggtcatcg gatatttttt cagcgccctt gcggtcaagc ttaccgaagc
     4021 gacattggga gcgaacgccc tcggactcgg ccttttggtc gggacggtcg ccggagcgct
     4081 tttcagtttg ctccacgcat tcgcctcgat caatctcaaa gcggatcagg tgatcagcgg
     4141 aacggcgatc aacatgatcg ccggtgcgct taccgtgtat ctagcccgaa cgatcaccgg
     4201 cagcggaaac gttcggatca tgatgggaat catccgaaag aatattccgg ttctctccga
     4261 aattccgatt gtcgggccgc tgttcttcac tcagacgtac tggacgacgt ggatctgtct
     4321 tctggtctgg ctcgtttcct atttccttct ttccaagacg agtttcggcc ttaggctccg
     4381 agcgtgcggc gaacacccct cggcggtcgc ctcggcgggg gtgaacgtcc atctgatgcg
     4441 ctatatcggc gtcatcatca gcggagcgct ttccggcttc ggcgggtcgg tgatcctcat
     4501 cacctacagc ggcgagttca acggaacggt ggcgggtctc ggattccttt ccatcgccgc
     4561 gttgatcttc ggacagtgga aagtgttggg aatcctggga gcgacgttct tcttcggaat
     4621 cgccaccacg atcgccaacg tcagtcaggt cattccttcc ttgatgatcg ttccgccggt
     4681 atacttcaag atcttcccct atgtagcgac gctcctcgcg ttggtagcgt tctcgaagcg
     4741 atcggcagca ccgctggcaa gcggtcaacc atattgagtc ggaggaatga atggataatt
     4801 tgatgaagaa gatgcaggaa gcggcgatgt tcatcaccag ccgcatcggc cagattcccg
     4861 tcgatatcgg aatggtcctg ggcagcggtc tgggtgatct ggcggatgat cttgcaaaca
     4921 gcatcgcaat ttcctataag gatattccgc attttcccgt ctccacggtc ttcgggcaca
     4981 aaggccgtct ggtcgccggc gatctggagg gaaaacgggt gctctgcatg caggggcgct
     5041 tccactacta cgaggggtat gggatggatc aggtggtctt tccgatccag gtgatgcagg
     5101 ccctcggcat caggcacctg ctggtgacca acgccgccgg cggggtgaac acctcctttt
     5161 ccgtcggaga cctgatgctc atcaccgacc acatcaaact gatcgcggac aatccgctgc
     5221 gcggtcccaa ccccgaccaa ctcggcgaac gcttcttcga catgaccgaa gcgtacgaca
     5281 agaagctgat cgagctggcg aaagctcagg cagaggagct ggggatcgcc gtccgagaag
     5341 gggtgtacat gttcttctcc ggtcctgcgt atgagacacc cgcggagatc cgggcggccc
     5401 gggttctcgg agcggacgcg gtggggatgt ccaccgtgcc ggaagcgctt gtcgccagcc
     5461 acatgaacat gcgcacgctt gggatcagct gcatcaccaa catggctgcc ggggtgctca
     5521 atcaacggct ttcccatcag gaggtgatgg agaccggcga gcgggtgaaa gaggacttta
     5581 cccggcttgt tcacgcgatc gtccgtcatt ggcccgtatg aatctgaaag agtatgccaa
     5641 aacatttgac ctcctccccc ttgaagggga gggtggattc ttccggcgag tccatacctt
     5701 caaggacggg agcgtcatct actacgccct tgatgaagag gatttttcct atacgcaccg
     5761 cttggtgacc gaggaggtgt ggtttcatct tgacggtttt ccggtgacga tcgtcctcat
     5821 cgatcccgag ggaaatgaag aggagcatct tcttgataag agcaatccga ttctcgtcgt
     5881 tcccgggctc tggtggcaaa gtgcccgccc caaggaagga gcggcgctct gcgccaccgt
     5941 gatgagcccg gcctggaagg aagaggagtt caccctcgcc gaggaagcgt tcgtcaacct
     6001 ccatccgaaa acgaggaggc tggtccaatg aaaacgatgc tgatcagcgg aggaacctcc
     6061 acgatcgggg aagcggccgt ccggtatttc actcaaaaag ggtggcatgt gtattttggg
     6121 tataacaccg ccagggaaaa ggctgaagcc cttgaaacgg aacttggccc ttccgccgcc
     6181 gccctccatc tgaacgttca agacaaggac tcgatcaaga ctctggcatc atcgatcgac
     6241 gagctggatc ttctgatcaa caacagcgga atcttctcct cctttttgac ggaagcgctg
     6301 gaggatgcgg aggtgggcag aatctttgat gtgaatgtca cgggcctctt tcggcttgcc
     6361 aaagcggttc tccccaaact gaaggaggga gcctcgatca tcaacatcgc ctcggtcaac
     6421 gcgcttatgc cgaccttcgg ctcgacgacc cactatgatg cctccaaagg cgcggtcatc
     6481 gcgtacacca gaagccttgc ggccgagctt gccccgaaga tccgggtcaa cgcccttgcc
     6541 ccgggcctcg tcgccgctcc gtatctcgat gaaaagaatg agattagaaa gatcttcgag
     6601 aagagaagcg tcctgaagcg gatggtgaca agcgaggagg tcgcccacgc cctgctcttc
     6661 atggcggaaa ataccgccct caccggggcg gtcattcccc tcgattgcgg gtatactata
     6721 gggtaggcat gaagattctt tgcatcagcg atgaaaccga tcctctgatc tactcggagc
     6781 acatcaccac ccgatatgga gatgtggatc ttgttctctc ggcgggcgat ctgccgctta
     6841 agtattatga gttcatcatc tccatgttga acaagcctct ctacttcgtc ttcgggaatc
     6901 acaaccagga gtatctgcat caataccttt caaacaatca gtcaccttcc gaggcgtttc
     6961 atcagcaagt cgtcggactg cagccgttcg gcggcgagtt cgtcgatggg cgggtgcgct
     7021 acgacaagcg caacaagctc atcatcgcag gtcttggcgg ttcgatgcgc tacaacaacg
     7081 gcgatcatca attcaccgac tttcagatgt tccggcggat cgtgaagatg atccctcgga
     7141 tgatcttcaa tcgaatcgtt cgcggcagat atgtggatgt cctgcttacc catgccccgc
     7201 cgctcgggct cggggacgga atcgatttgc cgcacaaggg attttcctca ttgttggtat
     7261 tcatgaagtg gtttaaaccg gcgtacctct tgcacgggca cgtgcatctg gttgacttta
     7321 atgcaaaccg tgttcatctc ttcaggcaga cgaaggtgat caacatcttc agaaactatc
     7381 tgcttgaaga tgatcaactc ggaggagaga cagatgtcca agatggttgg cgacctgctc
     7441 gcccagacaa atgaagattt tgaaaaagcc aagagcagag gtcgcattca atcgatcctc
     7501 tcttccctgg tccgcaagaa ctcggatttg ttgagcctct atgcggtaac ggatctgatc
     7561 aaacccaagg gcgagacata ccgggggatg cagacgattc cggtaaagaa tatcattgga
     7621 agcgaaggac gctatcagga tttctccctg gcgttctatc cgaaaaaaga gcttcttcgc
     7681 gcccgatggc gttcaatcga tctggcgacg aagaagtatg tgattcttcc gccgatctcc
     7741 gtgttcaagc ttggagagtg gtatttcgtt cgggacggca accaccgggt ctccgtcgcc
     7801 aagactcagg gcgtcgagtt catcgatgcc gaagtcgtcg agctggacag cgagatcccc
     7861 ctcgagccgg gtttgacgat gaaacagctg cgccgaaggg tcgtgtacta tgaacgcaaa
     7921 cgctttctgg agcagtatga cgtcgattgc cttccgatgg atcggatcat gttctccaac
     7981 gccggatcct atgccgagct ggtgaaccat atcctggtcc acaagtattt cctcaatgag
     8041 gacagcgacc gggagcttac cttcaaggaa ggagcgatct cctggtatac gaccgtctac
     8101 gaaccgatca tcgccgaggt ccggaagcag ggggtgctga cctccttccc cggaaacacg
     8161 gaaggggatc tgtacatgtg gatcgtgcgc cattgggaca acctcaagca tatgagcgga
     8221 agtcatgagg tgagcatcca aagcgccacc agcgactaca aggcgcgcta cggcaaaacc
     8281 ctcgtcggcc ggtggcttca taagctcaaa gagtttcttg gaaaggatta gtcctcctgg
     8341 atgtaagcgg cgaggatgtc ggcatacacc tcaagttcat gaccgagtcg gtcgccgggg
     8401 cggatcgaat cctcttttcc ggcaagatgg acgattcccg agaccggctg gtcgctgaac
     8461 tccagttcat agtgaacccc gaaggggatg tggagcatgc ccatcgattg cgcccagtag
     8521 atcagcgaat tgagggtgtg cgctccctcg cacgtcgagt tcgagcagga ggtgaagcag
     8581 gcgaagaact tcgtctccaa gcttttatct ttgctgagcg gcagcgtgga gtcgagaaac
     8641 gtcttcatct ccgccgagat gtttccgaac cgggtcggtc cgccgaggac gatcatatcg
     8701 cttttgtgca gagtggaatc tccgaccagc ggaagctcgc agatgtcttc gtagaactcg
     8761 ttggtcgttt cctgggtgtt cgcccagatg tggagatcgg ggtcgtcgac tcgatagagg
     8821 cgggcatcga cgttccgttc cgccatcaat tctttcagat agtttccgat gataaaacag
     8881 ttgccactga cgctatggat gataatcgaa caacgtttca tgaggtaccc tcgctttgta
     8941 tcgtataccc caagtataca ccaacccgag cgtttaagac cacaccgaat acagcccgtc
     9001 ccggtaatcg aggtagatcg gcgcctcata cacttcacgg agaagcgctt cattgaaaat
     9061 ctcctctttc ggaccgtcag ccacgatcgc gccgtttttc atcaccacca cccggccgat
     9121 agcagggatg atctcgctga gctcatgagt ggcgagaata atcgttttcc cttcccggtc
     9181 gagctgctcg aggcttttgc gataggtgtg gcggctgggg aaatccaacg aggcgcccgc
     9241 ctcatcaagg agcatcacct ccggctcatg caccgccgcc cgggccaaca gaacccgacg
     9301 cgcttcgccg ctggagaggc ggttcatctg tttctcccac agcgggagaa tcccgtaggt
     9361 gttcatgacc tccttcgccc gcttaaggtg ggattcttcc acgagatggt ggaaatccaa
     9421 gccgatcgaa ctgaagaaac cgctgacgac gatctcccag gccttgtagg tggatgtgca
     9481 gacatgttga aggctcgccg atacgatgcc gagatgcttt cgcagctcca ggatctgcca
     9541 tcgggacttt ccaaagagaa tccgtttgaa atcgggggta tggaggggat ggacttccat
     9601 gctgagcgtc tgaaccagcg tcgactttcc cgctccgttg ggaccgatga tcgcgacgtg
     9661 ttcgttccgc ttgacggtca gggagacatc gttaagaatc cgctggccaa ggcgctggac
     9721 ggtagcgtgt ctgagttcaa tgagggtgcg catacggcga gtatagcacg gctaggcgta
     9781 cgaggcgcgg cgttgttcct caggatagga atcgagaact ttgtcctcca gcgtggagag
     9841 gatgaccatc accaaggcga ccgcgaggtc ggtctggccg ttttccagcg cttcgcagag
     9901 gtaggcgtag tttttcgccc gtttgtccaa ttgaccggcg ctgaagcgga tggaacgcat
     9961 cgcctgatcg agaaggggag agaggttctc atagatggcg cttaagatct ggttgttgtt
    10021 cgccatcacc agggtatggt ggaaatccat ctcggcgtcg aggaaggatt gggtcgcctg
    10081 agcttcccgg tcatagattc cggggaggga gacctccatc tgtcggctcg ccgcccacag
    10141 ctgggtaaga tatcgctttc ttccttcgag acgggaaaag ttccgagctg cgctgctggc
    10201 gacggtaagg cgcacctgca tcaggttctt caacagtttg gagtggctaa tgtcgctgtt
    10261 cagaattgag gaggagacgc tcattacata gtgatcaagg ttgttggagc ggatctgagc
    10321 ccgccggccc tgggaaagaa ccaccagtcc ccgggcttcg agaatcttca acgcttcccg
    10381 gattggacgg ctggatgtcc taaactgttc tgcaagttcg acttgagagg gtagatagga
    10441 gccgcactcc aatgtgccgt ctttaatcca tcgttcaatc gtctgggcta ttaccgtgga
    10501 tttggtctgt ggaaggtcgc tcatctatag tatccctcaa aaatggtgtc atacgttgga
    10561 agtttgttcc taaacagtag tatacaacag aacctaaata gttacaataa ggcgatatct
    10621 acaattttga tagatgacat ctgttgttat tatgtactag gagagataga atctttacta
    10681 cttaagacac aagcattagc ttagaactca agcgcttcga atttctcgct gatatttgtc
    10741 aggttcacga gagtgagagc gaccgccagg tcggtctgac cgttttcaag ggcatcaacg
    10801 aggtagcggt attcgtttac cttcttctgc tgttccttga acgtctcggt aaggcgttcc
    10861 atcgcgctgt acagctgcgg ggcgaggtag tcgtaaatat gggtgaggat cgcgttgtcg
    10921 ttggatttga tcagggtggc atggaactcc agctccacat ccttgaattc ggcgagcgct
    10981 tcctcatctt tactgttctc aagggaaggg aggatctgct ccattcgggt gcatgcccgg
    11041 ctgagggaac ggacgatgag ggttcggttc ggatcgcggg aaagctcgcg ggaggcggag
    11101 acctcgatcg tggttcgtac ctgcatcagg tcccggatca tcttcttgtc catcgcgctg
    11161 gtgctgatca tcgagctggt aagggtttcc acaaagcgct caaggctgtt ggtctggact
    11221 tccgctcttc gaccctgctg gactttgatg aggcctttgg cttccaggct cttgaacgct
    11281 tcccgaattg agcggctcga agcgccgaac atctcggcca gttcctgctg gctgggaagg
    11341 ttgcctccgg ccggatattt tttagccaga atgtcttctt ccaattgcgc ggcgataacc
    11401 gatgattttg tttcaacaac cttagccata tgtgtctccc agtcgaatat ggttcactac
    11461 gtgaatatcg tacgacattt tatacaaata tgcaataata aatgtcataa gttagaagtt
    11521 ttttacaaaa ataacttttg gtaatacaag agaataaaac aagagtgtta ttatagtaac
    11581 tccttataat gtaagaagta tcgtctcaaa acctgcatat tcccccgaat cggttaaaaa
    11641 tcgactgaaa acatttatca aaagacgatt ctctgtaaaa ttatatataa tataaaaata
    11701 aatcaaacag tattcattat tattaataca taatacgaaa tacttgacat atgtttcggc
    11761 tctttttcct gaaaaaggac gccaaacctg acagaggttt cacttgtgtc catatattgt
    11821 atacataata atcgaagacg gtgactggag aacagcggct aaaaatgaat atagtgaaac
    11881 atatgttata tgttgaaaaa cagataattc tatataaaat aataaaataa aaatattatt
    11941 gcaaagattt gaaatatttt tatacttttt attcagaaga gcttgacata tgtgctagga
    12001 tgccgtatag tgagattaca aaaacgaatt atatgggagc atagccccca aaaggaaaag
    12061 gaaaagacta tgaaaaaact aattgctttg ttaatgatcc tggcagtggt tctgacgggg
    12121 gtgtttgcgg agcctggtga cgaacaatct caggtgaagg tttatcttaa aggtacgata
    12181 ggagcatttt ttaggcacgg cgtgaaggaa aacgatgcgc ttgtttatga gaagacgtac
    12241 caatcggaaa gtgttctttc tgcgaccgga acgaatttcc agtatggata tcaatccaac
    12301 caagacatat tgggtattct gtatgtggat tacactgatt ttagtgatgg caacaatcat
    12361 agcatccaga tcggaaccct taagataggc ggtaccgcga tgactgctga taactacact
    12421 cagggtagtg ggtttaaggt atttactaat ttgacaggta ccaacatgag tattcaagaa
    12481 aaggctattc aggtcattgc tgctcaggta aaggcaggga atgatcccaa gggcacagcg
    12541 atcagtcata tcgttggcga ggctcataag ggcacgtaca ccgcaaccct tacgtttcaa
    12601 gtcaaagcta actaatacga ttttgatttc tgacaatccc aaagctgtgc tcttgcacag
    12661 ctttgattgt ttcagggctt cagattaaat tctttctttg atagaatgag gatgtcatga
    12721 agagattact tctattgagc ataactttat ttcttcttct tccgttggcg gggaggacga
    12781 tcaccgccgg gctgggcgaa cctgtggcgg tcagcctgaa ggggacaatc gactctaagg
    12841 agttgacgat ctcggtgaga ggggaaggaa tggatgagga gctcaatgaa tacgatgtct
    12901 tgatgtttga ctttcccatc accgaccatg ataaaagatc gtgggtgaag gaagttcctc
    12961 tgcactttac ctactcatcc aataagaatc tgccgagtaa agcacatttg gaattcgtcg
    13021 ttggcgagtt tgtcggagac agcggtttca aactgaaacc cgagctgaaa atcgccagat
    13081 caagcaacaa gatcgatctg gattccgatc ccagacggat tctgcttgaa acgaccttcg
    13141 agtccggtcg gcaggaagat acaccgattg tgaccgtggt cgtcgtcttt accaaggctg
    13201 aagaggaatt attcccggtc ggaaactata ccggcgtgtt ttccatcaat tatctgggaa
    13261 taaactgatt atcctcttgc gctgtccggg accattccct gttttcgtgt ttttgccacc
    13321 agattcttca gttcccgggc cgtatatcgt actttcacta agtctaaatc catcgcatca
    13381 ccaatggcct tgatgatgtt cgtcggttcc agaagcaggt agacatcatc cgtctcctcc
    13441 accacctgca atctccgtag atcatcgacc atcttttcga actcaatgct cttccccgtc
    13501 ttcagctcca gaattttcag aatgagcaag ctcacgaacc tgcttagcag gtgggcttcg
    13561 atggattctt tctcttggtt gcttccgaga aagcgttcct cgattcgcca taggccccta
    13621 tacatatcaa tgatatccaa agccgtcgca ggcttctcga gccggaagag cggtgatgag
    13681 ggatcgcctt cggagccgat gacatttgtg tggattacat gatacccgtt ctcctcccgg
    13741 aagacgatct gacgttcatt gaccgtcatc cgctccgaag cgtttggatg tttgtatccc
    13801 gtctcatccg gaatatacgc cttggcatcg gcatcggtct tcgatagggg ggtgccgaag
    13861 atgtaccctc ccttgttggc caatatctcc tctttatcgt tggcgctaat catccctttg
    13921 tctgaaacat ggacaacctt ttggatttcc aattgttcat cgacctcttt caccatccgt
    13981 gcgagggtcg atccatcatt accgaaaagt ccataggcga tcggcagccc attgtcgtcc
    14041 gtcaacagac ccatttggat gtgatgctct tcgcctgctc ttccgttcgg caccctcgct
    14101 ctatacagca ccgatggatc tcgcccgtac agcgtgccga ccttccggtg cagatgcttc
    14161 tgcaatgccg tcttatgggc ttcgaaaaag ggaagagcgt gccggacatc ctcatcggtg
    14221 aaatccatgt tcgagagcag tcctagacgg cttcgccagg tgcttaattg cgattccgga
    14281 aagagaatcc ctccaaagac cagggcttga aagattgcat tgtggttgta ccgggctttc
    14341 gtataccgcc ttcggttatc ccagaaagac ccgatctcca attcatgata gatctgcgag
    14401 aggggaagca tcccaagtga aagaccgggg gctttcacct ctctgctttc cgcatcgaat
    14461 tgatggtgtt catccaggga cacttcaacg ttgagcttcc ggcggtcgct tcttcgtttc
    14521 gcctcttcct tgaaatgagc aatcggatca tcatagatct tctccagttc atccagatag
    14581 ccgattcgct cgactgttct ggtccggttc tttcccttga cccgcttggt ttcctgaaac
    14641 agtaataatg tccgtccggt gctgcgctcg aaaagcttgt tcaggtacat ggcaccagtg
    14701 tatcacatct gatatccggg gaaatacgga taatcggcaa tatttgtttt acagcttaca
    14761 gaatccatgc aataataacc cgtcttccgc agttcaaaga gttaaacaga ggccataacg
    14821 aattgtat
//
LOCUS       DAOSLI010000043        13935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_4478_length_13935_cov_27.898271, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000043 DAOSLI010000000
VERSION     DAOSLI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13935
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4478_length_13935_cov_27.898271"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..523
                     /locus_tag="PLF67_06845"
     CDS             98..523
                     /locus_tag="PLF67_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89766.1"
                     /translation="MKILFPNAVDVGCSDFVCMHGEVVQPLSVLQATEADEQLEDRPQ
                     ARPVFVWTDEHIKGYFVMCYLALSMIRYLQYLMGEEGWKEVLSAEEIMTALKKPQVVV
                     QGTYPGQCQPRVPKHSEAPEYEGSSQEHDSYPVPLSHEA"
     gene            complement(606..1469)
                     /locus_tag="PLF67_06850"
     CDS             complement(606..1469)
                     /locus_tag="PLF67_06850"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE89767.1"
                     /translation="MSRKAMSLKAKIRNLAKEKGMLAQVILQNYMFERFLDRLSKSNY
                     KNNFIVKGGMLIAALVGISNRSTMDMDTTIKNYPVTTDSLTLAITEICHVGVDDDVNF
                     SFIGIESIRDDDIYGGYRVGIRADYDTITTPLSVDITTGDIITPAEVFYSFKKLFDEG
                     TIGVWAYNIETVLAEKVETILQRGELNTRLRDFYDIYILVNTQDFDRSVFSDALKRTT
                     EHRKTTYIFNDTLKRLKTIETSEILKDRWTRYSKNYPYAETIDYEEVIKTLKMLLLTF
                     GLAERKISRKG"
     gene            complement(1466..2254)
                     /locus_tag="PLF67_06855"
     CDS             complement(1466..2254)
                     /locus_tag="PLF67_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOE89768.1"
                     /translation="MYRWLVHIQIQLILGGVSALTVLAEEERPEQGIFKGSFLILYAN
                     HVYKHTYINNNISIFVKQVKAMDHTKRIKTIINKQQGTLLSSDVDEYNIPRTYVAMMV
                     AEGTIERIDRGVYVLPDSLEDWMLILQKKYPNLIYSHETALFLHGLSDRTPFEYSATV
                     PSGYKVVNAIREKAKIYYIKKELHLMGVTEERTPLGNTITVYNVERTMCDVVRSRNRM
                     DLQIVSNALTRSLKKKNVDYALLMGYARKLNVEKLLNMYMEMLT"
     gene            complement(2451..3779)
                     /locus_tag="PLF67_06860"
     CDS             complement(2451..3779)
                     /locus_tag="PLF67_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOE89769.1"
                     /translation="MIAIAFPILVQSLLNNSLSFVDTLMIGQLGETAIAAVALANQVF
                     FLISLLFFGVASGTSIFLSQYWGAKDTRYIQKIMALSITFVAFFASIFALLSLFIPEA
                     IMHIFTREAEVVSGGVSYQKIVAVSYIFGAITQIFSTALRVTGHAKTPLKIALLALSL
                     NAIGNYFLIFGIGPFPVMGIAGAALATTLSRLVEVVLLFIVVYRKHPHLAIRDREAFL
                     WPRSFILHVVPTVLPVLLNEIAWALGMTTYKIAYSRIGIDAIAAINVAESVGNLFFVA
                     LLGLSNATLIMIGIKIGEHDYPLARKWSRRFVLVAVTVGAVMGGFELLFAPFFAGMFN
                     ISPELQATAVRLLMIHALTLPARAFNITVIVGVLRAGGDTRFSMLAEMFGVWAIGVPL
                     AFIGVHLFNLSIVPLYFLVSMEEISKMLIGLGRLKSGKWLNDLTVHAHDV"
     gene            3970..4899
                     /locus_tag="PLF67_06865"
     CDS             3970..4899
                     /locus_tag="PLF67_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOE89770.1"
                     /translation="MPESELFVILNPYAGKGQARKLEEKVRFSLSRGERKVVLIRTEE
                     EHEAERLAWEATMDGRKSIIAAGGDGTVNEVVNGMIKASLEMGTPPPVLGVIPIGRGN
                     DFAWGMGLPLDLEEACTVAFAGKTKMIDVGKVYGGKYPDGRYFVNGLGIGFEPLVNFM
                     ASDFKRLSGAPSYILALLKMLARYPKPYRVEITYDGKQRIVWTQQLSISNGRRMGSLF
                     LMGPDAVFDDGFFDVMWAKRPVSRRKIIPVIISFLKGTQLKRPDFKMVRTKKFSVVAK
                     RGLLPVHVDGEEISRGCTEISAEILEGILPVFV"
     gene            5167..6591
                     /locus_tag="PLF67_06870"
     CDS             5167..6591
                     /locus_tag="PLF67_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOE89771.1"
                     /translation="MSKDLVPFVHFYDQDFVDMYDRSWVWIDELWREGSKENQFSGSY
                     LSYPDQKYFSQYLSSFSSLFLVYSNQNHSPFAMLDYFYTRQEESGAIRGEYSIEDGRP
                     VLTEENPEGFSPPLFAYVEYIFYHKVGNKKRLRDVVPILERYFAWLEETALQENGLYH
                     VPTEACQTGNIPRSETFYPVDFNSQLALSALYMSAIGDILNDKEISFRYKRLYFSLKT
                     RINAHMWDDEDRFYYDLDKDGQRIKSKLLVSYWAMLAEIPNDERATYLIEQLQDPNEF
                     GTENPFPAVPVSSPCYSEEGNGHCGGVFSVHTYMVVKALERYNHFIYARECAIRHMYF
                     LLDTLHPDTDQIGDLWEAYLPNKEGPSKTDEIEGFPRRRLMHYAGLATITLMIENIIG
                     LDISLPRKTVDWVMPSLEVMGIENLSLKRNTITILSNKTDRGWEIRLESEKLYYFTIE
                     VLNEKKKKTLPIPSGKCSMLIDKM"
     gene            6597..7343
                     /gene="djlA"
                     /locus_tag="PLF67_06875"
     CDS             6597..7343
                     /gene="djlA"
                     /locus_tag="PLF67_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HOE89772.1"
                     /translation="MGWLNKFIGGMIGLALGGPLGMIAGVAFASVIDKAGRVQERTNP
                     LDQQQQLFFVGAFSLLAYVAAADGSVTATERQKVVEFIRRDLRLDYQTEELALRVFDA
                     ALNSNQSVEAVASQFYQGYRNNPAILQLMVDICYRVAYADGTISSKEEALILKIARLF
                     HFSEPLIETFQKRYGLATTSLEQAYATLGLSSGATNEEIKRAYRKLSMEYHPDTLISK
                     GLGEEFLKAATEKFRAIQDAYTRIKQERNL"
     gene            complement(7340..7930)
                     /gene="recR"
                     /locus_tag="PLF67_06880"
     CDS             complement(7340..7930)
                     /gene="recR"
                     /locus_tag="PLF67_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOE89773.1"
                     /translation="MTSLDTLIKDLSRLPGIGPKSATRLAYHLLKSDPSYNRNLAEEI
                     ATIQERIKPCSVCGNYTETDPCSICSDPNREQTIICVVEEPQDVLTLQASGAFDGLYH
                     VLGGAISPLDGIGPERLSFGKLMKRIREGSFSEVIIATNPTEEGDTTALYLRHLLKDH
                     PAITVTRLASGLPIGGDLEYADRVTVSRSLRGRVSF"
     gene            complement(7927..8220)
                     /locus_tag="PLF67_06885"
     CDS             complement(7927..8220)
                     /locus_tag="PLF67_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOE89774.1"
                     /translation="MNPFELLKNAQAIKEQMARMQDILPAIRATGNAGAGMVTITLNG
                     KFTVVDIAIEEAAAEDLETLQLLIQSAFNDASAKLQQKLQDEGLKAVSMMGQS"
     gene            complement(8220..9797)
                     /gene="dnaX"
                     /locus_tag="PLF67_06890"
     CDS             complement(8220..9797)
                     /gene="dnaX"
                     /locus_tag="PLF67_06890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOE89775.1"
                     /translation="MSQYEVTATKKRPQEFSTLVGQPFVVSTIEGAIAQGRIAHAYLF
                     SGPRGVGKTSAARILARSLNCDEGPTTKPCGVCSHCIEIKAGNSVDVIEIDGASNTGV
                     NDIRQIKDEVLFPPQTGRYKIYIIDEVHMLSISAFNALLKTLEEPPPYVIFILATTEL
                     QKVPATIRSRCQQFHFQLIGLDLIKESLAQAAVDQDIQADDDALFWIAREATGSMRDA
                     YTLFDQVVAFSAGHITMETIRSKLGLVGFDRIITIATDLLEGNTQTALLAIHSLINAG
                     VSVEQTIKDLTQFYRSLLFMRSQINDEAILGVQSDRVPLALRTAYTTEQLEAALELLL
                     ATYREIRYSLNPRIELELAISRLGELKSLRSNTALVKEIATLREELLSGNVHVTRKVQ
                     APAPPPKQQERITPAAPPSRPSRTLTMEDLPELTRRLGDAPMLARVLGTVKELNQNDR
                     TLTFTFNTAFAATTAKENSERLVKAIEELTGPGVDVYCINGETSKASEESPSGEDPVL
                     RNIATVFRGDVLTRKSQ"
     gene            complement(9854..9952)
                     /gene="ffs"
                     /locus_tag="PLF67_06895"
     ncRNA           complement(9854..9952)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLF67_06895"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(9965..10047)
                     /locus_tag="PLF67_06900"
     tRNA            complement(9965..10047)
                     /locus_tag="PLF67_06900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10011..10013),aa:Ser,seq:gga)
     gene            complement(10067..10151)
                     /locus_tag="PLF67_06905"
     tRNA            complement(10067..10151)
                     /locus_tag="PLF67_06905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10115..10117),aa:Ser,seq:tga)
     gene            complement(10167..10240)
                     /locus_tag="PLF67_06910"
     tRNA            complement(10167..10240)
                     /locus_tag="PLF67_06910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10204..10206),aa:Arg,seq:gcg)
     gene            complement(10259..10345)
                     /locus_tag="PLF67_06915"
     tRNA            complement(10259..10345)
                     /locus_tag="PLF67_06915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10309..10311),aa:Ser,seq:gct)
     gene            complement(10381..10468)
                     /locus_tag="PLF67_06920"
     tRNA            complement(10381..10468)
                     /locus_tag="PLF67_06920"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10432..10434),aa:Ser,seq:cga)
     gene            10681..10998
                     /locus_tag="PLF67_06925"
     CDS             10681..10998
                     /locus_tag="PLF67_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE89776.1"
                     /translation="MAKRTPVLLPWVERILIDLGERIRLARLRRNISAQLLADRAGIS
                     RQTLSTLENGSGSVSLATFIQVLFVLGLEKDLEEVAKDDVLGRKLQDLNLPTRRRATK
                     RDR"
     gene            11096..12409
                     /locus_tag="PLF67_06930"
     CDS             11096..12409
                     /locus_tag="PLF67_06930"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOE89777.1"
                     /translation="MGKNEAPPSSRKKTKLATFKAGRLAQRALAFTKEVLAQSGPRKS
                     GSLAALEAAELIKSELETTCDTVESQQVPIDSKINTRLFTLVVYTYPAVVLLCLVGLP
                     YLGLLLSALVLWFSFETFGRYKPRYLAPTDMGVNVHGIIEPVKAVESTIIFTAHHDSA
                     PIERFEKKKAMIPFGLVGGAALLSFLQTAVELTQLRLFRPNFPPILLFLLGLGLLGGL
                     YYVRTLLEYYTDEVAPGAGDNLISVGILAALAQYFKAEPLSKTRIILASFDGEEIFLQ
                     GSTAWYEAHKEALEGAVVLNFDSIFSAEHLVFLERDANATLPLSKSLARKCVNIARSM
                     GYEASALSMPILGGATDAASAARIGLEATTLTASSWSDGVYHTKDDTLAAIDPESVER
                     SIGIAIRLARLLDQDSLWDDDLPIEEEKVEEPTNPTLVFSKLTRR"
     gene            complement(12396..12989)
                     /locus_tag="PLF67_06935"
     CDS             complement(12396..12989)
                     /locus_tag="PLF67_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOE89778.1"
                     /translation="MLILKKEATVEIEVRKSRFIAHAIPITSLDQIKGLVQKAREEHP
                     QAVHVVHAAVVGPKGDLYSFSDDREPRNTAGRPAFEVLKGSGITNILILIIRYFGGIL
                     LGTGGLVKAYGQSVRELLPLLETEELIEKTAFRVTVPYNLYELLKLQLVSLEAEIESE
                     EFTTDVTITATLPATDIEDLNLYVRNLTNGGASLIVE"
     gene            complement(13013..13912)
                     /locus_tag="PLF67_06940"
     CDS             complement(13013..13912)
                     /locus_tag="PLF67_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOE89779.1"
                     /translation="MILETIERFYSWALIGILLLNLTQRKIPNSHKKRTATLYIASVL
                     LLFQLGLITILTKGWSHYLAIPLALLCVLIIFLLRKRAWPFKLRCVECSAKLDFNHVI
                     GGDENLCKTCWEIAHPQQAEQEDPIEVTIDVQDVESVEQIDWEIWEPSEICVITYLFD
                     DDRVLLIDKKRGLGRGLVNAPGGHIEEDETAMEAAIREFKEETRLDISDLEMVGVLNF
                     QFRDGLAEKAYVFFTRTYTGELAETDEARPFWVPISEIPYDRMWEDDLYWLPPALEGK
                     HFEGFFIFDGQTMVDKRITFKTP"
BASE COUNT         3218 a         3571 c         3725 g         3421 t
ORIGIN      
        1 ttttagctgt cgttctcttg tacccgtata agccgtcaca gccggatatg aaggcatgtg
       61 atgagcctga cgatgcggga atcatcctga acgaaccatg aagatcctat tccccaacgc
      121 tgttgacgtt gggtgttcag acttcgtgtg catgcacggt gaggtcgttc aaccactttc
      181 cgttcttcag gcgaccgagg ccgatgagca gcttgaagac cgacctcagg caaggccggt
      241 gtttgtctgg acggacgagc atatcaaggg gtacttcgtc atgtgctacc tagccctgtc
      301 catgatacgc tacctccaat acctgatggg ggaagaaggc tggaaggaag ttctttccgc
      361 cgaggaaatc atgactgcct tgaagaaacc acaggtggtc gtacagggga cctaccccgg
      421 tcaatgtcag ccaagagtac ctaaacatag cgaagctcct gagtatgaag gatcttcgca
      481 agaacatgac tcttacccag ttccgctcag ccacgaagct tgacctgaac aggatcctga
      541 gctgaattta gtaggagatt ttattttctt acttttccct tgaaaacatc atttggtgat
      601 gccattcagc cttttcggct gatttttctt tcagcaagtc cgaaagtcag gaggagcatt
      661 tttagagttt ttataacctc ttcataatca atagtttctg catatggata gttttttgaa
      721 tatcttgtcc accgatcctt taatatttcg cttgtttcaa tggtcttcag tctttttaga
      781 gtgtcattaa atatatatgt tgtttttctg tgttctgtcg ttctcttcaa tgcatcagaa
      841 aacactgatc tgtcaaaatc ttgagtattg actagtatat aaatatcata gaaatccctt
      901 agtctggtgt tcagctctcc tcgctgtaat attgtttcca ctttttctgc aaggacagtc
      961 tcaatgttat aagcccatac tccgatagta ccttcatcaa aaagcttctt aaacgaataa
     1021 aaaacttctg ctggggttat aatatctcct gtggttatat caactgaaag cggcgtagta
     1081 atagtatcat aatccgctct aattcctact ctgtatcctc cgtatatatc atcatccctt
     1141 atagattcaa ttcctataaa agaaaaattt acatcatcat ccactcctac atgacatatt
     1201 tctgtaatgg cgagtgttaa tgagtctgtg gttacaggat aatttttaat tgttgtatcc
     1261 atatccattg tggatcgatt ggatattcca acaagagcag ctatcaacat acctcccttt
     1321 actatgaagt tgtttttata attagatttg gatagacgat caagaaacct ttcaaacata
     1381 tagttttgca atatgacttg agccaacatg cctttttctt ttgctaagtt cctgatttta
     1441 gctttcagac tcatggcttt tctgctcacg tcagcatctc catatacatg tttaacagtt
     1501 tttccacatt gagttttctt gcatatccca taagtaaggc gtaatctaca ttctttttct
     1561 tcagcgatct tgtaagggca ttggaaacta tttgtaagtc cattctgttt ctgcttctta
     1621 ctacatcaca cattgtcctt tccacattgt atacggttat ggtgttccca aggggtgttc
     1681 tttcttcagt aacacccatc aaatggagtt ccttttttat ataatagatc ttcgcctttt
     1741 cacgtatggc gtttacgact ttatatccgc ttggaaccgt tgcagaatat tcgaagggtg
     1801 tcctgtccga aagcccatgc aaaaaaaggg ctgtttcatg ggaataaatg aggttgggat
     1861 atttcttttg taaaatcagc atccaatctt ccaatgaatc cggcagtaca taaacgcctc
     1921 tatctattcg ttctattgta ccttctgcaa ccatcatggc cacataggtt cttggaatgt
     1981 tatactcatc tacatccgaa ctgagcagcg ttccctgttg cttgtttatg atggttttaa
     2041 ttcttttcgt gtgatccata gcttttacct gttttacaaa tatacttata ttattattga
     2101 tataagtatg tttgtaaaca tgattcgcat aaagaataag aaacgacccc ttaaaaattc
     2161 cttgttctgg acgttcttcc tctgcaagaa cggtaagagc gctcactcct ccaaggatca
     2221 gctgaatctg aatatgtacg agccaacgat acatggggtt ttactgtaag actctgaacg
     2281 aaaaatgata gtcctattcc ccaaatctgt cagaatgttg aaattgggaa tggaattgtg
     2341 atccggttgt gaaaagctga cgcttgtata gaaccgaaac cctatggaag acagtggggt
     2401 gttaacaagc ggtcgtcggg gcgggtgagg atatcttgca gggttggtgt tcagacatcg
     2461 tgtgcatgaa cggtgaggtc gttcagccac ttcccgctct tcaggcggcc caagccgatg
     2521 agcatcttgg agatctcttc catgctgacg aggaagtaga gggggacgat cgagagattg
     2581 aagagatgga ctccgatgaa cgccagggga acgccgatcg cccagacgcc gaacatctcg
     2641 gcgagcatgc tgaagcgggt gtcccctccg gcgcggagaa ccccgacgat gacggtgatg
     2701 ttgaatgctc gagccggcaa cgttagggca tggatcatca gaagacgaac cgcggtagcc
     2761 tgaagttccg gactgatatt gaacattccg gcgaagaacg gggcaaaaag cagttcaaag
     2821 ccccccatta cagctcctac cgtgacggca acaaggacga atctccgcga ccacttgcgg
     2881 gccagaggat agtcgtgttc cccgatcttg attccgatca tgatcaaggt ggcgttcgac
     2941 aggcccaaaa gggcgacgaa gaagaggttg ccgacgcttt cggcgacgtt gatcgcggcg
     3001 attgcgtcaa tgccgatcct cgaataggcg atcttgtagg tggtcattcc caacgcccag
     3061 gcgatctcat tgagaagaac cggcaacacg gtgggaacca cgtggaggat gaacgaacgg
     3121 ggccatagaa acgcttcacg atctcgaatc gccagatggg ggtgtttgcg gtagaccaca
     3181 atgaagagaa gcaccacctc gaccaggcgg cttagcgtcg tggccagcgc cgctccggcg
     3241 atccccatga ccgggaaggg gccgatgccg aagatgagga agtaatttcc gatcgcattc
     3301 agggagaggg ccaaaagagc gatcttcaaa ggagtctttg catggccggt aactcgaagg
     3361 gcggtagaga agatctgcgt tatggcaccg aagatgtagc tgaccgccac gattttctga
     3421 taggaaactc cacctgagac gacctccgct tcacgggtga agatgtgcat gatcgcttcg
     3481 ggaatgaaca gggaaagaag ggcgaagatt gaagcgaaga aggcaacgaa ggtgatcgag
     3541 agcgccatga tcttttggat ataccgggta tccttggctc cccagtattg agagagaaaa
     3601 atcgacgttc cgctggctac tccgaagaaa agcagcgaga taagaaaaaa cacctggttt
     3661 gcaagggcta cggcggcgat cgcggtctcc ccgagctgac cgatcatcag ggtgtcgaca
     3721 aaggagaggg agttgttcag caggctttga acgaggatcg ggaacgcgat tgcaatcatt
     3781 cttcggtaga atgatcggcg ttcgagggat tggggcgata gcgttgtact tggcacgaga
     3841 agcaactccg gaataattag gagcgcagta tagtatatcc tccgggcttt gcatataaga
     3901 cttcagcaga tatgaaattt gtggtacgat cgcactaagg ctttgtaaaa tacgttgtga
     3961 ggatgctata tgcctgaaag cgaattattc gtgattctga acccctatgc cggcaagggc
     4021 caagcacgga aattggaaga aaaggttcgt ttctcccttt cgcggggaga gcggaaggtt
     4081 gttttgatac gaaccgaaga ggagcatgaa gcggagcgcc ttgcctggga agctacgatg
     4141 gatggacgga agtcgatcat tgccgccggc ggagacggaa cggtaaacga agtcgtaaac
     4201 gggatgatca aggcatcctt ggagatggga actccaccgc cggtgctggg agtcatcccc
     4261 atcgggcgcg gcaatgattt tgcctgggga atgggtttgc ctcttgatct tgaagaagcg
     4321 tgcacggtcg cttttgccgg aaagacaaaa atgatcgatg tggggaaagt ctatggggga
     4381 aaataccccg acggccgata cttcgtgaat ggtctgggga ttggctttga accgcttgtc
     4441 aacttcatgg ccagtgactt caagcgctta agcggagccc cttcatatat tttagccctc
     4501 ctgaagatgc tggcaaggta cccgaagccc tatcgggttg aaatcaccta tgacggaaaa
     4561 caacggatag tctggactca acaactctca atctccaacg gaagacggat gggctcgctg
     4621 ttcctcatgg gtcccgatgc cgtctttgac gacgggtttt ttgatgtgat gtgggcgaaa
     4681 cggccggtca gtcgacgaaa aatcatcccg gtcatcattt cttttttgaa gggaacccaa
     4741 ttaaaacgac cggatttcaa gatggtgcgg acaaaaaagt tctcggtggt cgccaagcgg
     4801 ggcttgcttc ctgtccacgt cgacggggaa gagatcagcc gcgggtgtac ggagatttcc
     4861 gctgaaattc tcgaaggaat tttaccggtt tttgtctgat ttctcaaaag catgtcaagt
     4921 aggcgccctt tgaggtagtt ttgttccgtc tttcacaaaa gccgctcttt ccttttcaag
     4981 cacaattagc tattatacaa atgtttagct gagaatagcg caagcgctgt gctttgattt
     5041 cgatttttcc ggttcaacgg aactcctata gatttttaga ttgacatctc atagaagaaa
     5101 gtttatctta aacaatgcac gtttttaggg tttttctgcg accatagcta tataaggagt
     5161 ctcaatttga gcaaagattt ggtgccgttc gtacacttct atgatcaaga tttcgttgat
     5221 atgtacgatc gctcatgggt gtggatcgat gaattatgga gagaagggag taaggaaaat
     5281 cagttttcag gctcctatct ttcctatccg gaccagaagt atttcagcca atatctctct
     5341 tcattctcct cccttttttt ggtgtattcc aatcaaaacc actccccgtt cgcgatgctc
     5401 gattacttct acactcgcca ggaggagagt ggtgcgatcc gcggtgaata ttccattgaa
     5461 gacggcaggc ccgttttaac ggaggaaaat cccgaaggat tctcaccgcc gctgttcgcc
     5521 tatgttgaat atatcttcta ccacaaggta ggaaacaaaa agcgcctgag ggatgtcgtc
     5581 cccattctcg aacgatactt tgcctggctt gaagaaaccg ccctgcaaga gaacgggctg
     5641 taccatgttc cgaccgaagc gtgccagacc gggaacatcc cccgctctga aacgttctat
     5701 cccgtcgact tcaactccca gctcgccctg tccgcgctct atatgtcggc gatcggggat
     5761 attctcaacg acaaggagat ctcctttcgc tacaagcggc tctacttctc gctaaaaacc
     5821 cggatcaacg cccatatgtg ggatgatgag gatcgcttct actacgacct cgataaagac
     5881 ggacagcgga tcaaaagcaa gctgcttgtt tcctactggg cgatgctcgc cgagattccc
     5941 aacgatgagc gggcgaccta cctgatcgag cagcttcagg atcccaacga gtttggaacg
     6001 gaaaacccgt tcccggcggt accggtgtca tccccctgct acagtgagga gggaaacggg
     6061 cactgcggcg gggtcttcag tgtccatacc tacatggtcg tcaaagcgct tgagcggtat
     6121 aaccacttca tttatgcccg tgaatgcgcg atccggcata tgtacttcct tctggatacg
     6181 ctccacccgg ataccgatca aataggggat ttgtgggaag cgtacctgcc caataaggag
     6241 gggccgtcga aaaccgatga aatcgaaggg tttccccgcc ggcgtctgat gcactatgcc
     6301 ggtttggcta ccatcacgct gatgattgaa aacatcatcg gtcttgacat ctcgcttccc
     6361 cgaaaaacgg tggattgggt gatgccttcg ctggaagtga tgggaattga aaacctttcg
     6421 ctgaagcgga acacgatcac gatcctttcc aacaagaccg atcgcggatg ggaaatccgc
     6481 cttgagtcgg agaagttgta ttactttacg atcgaagttc tcaatgagaa aaagaagaaa
     6541 acccttccga tcccctccgg caagtgttcg atgctcatcg ataaaatgta aacgcaatgg
     6601 gttggcttaa taaattcatc ggcggcatga tcggcttggc gctcggcggt cccttgggga
     6661 tgatcgccgg agtcgcattc gcctcggtga tcgacaaggc gggccgggtt caagagcgaa
     6721 ccaatcccct cgatcaacaa cagcagctct tctttgtcgg agcgttttcc ctcttggcct
     6781 atgtcgctgc cgccgacgga agcgtcaccg cgaccgagcg tcaaaaagtc gttgagttca
     6841 tccgccgcga tctccggttg gattatcaaa ccgaggaatt ggctcttcgc gtcttcgatg
     6901 ccgccctgaa ctccaatcaa agcgtcgaag cggttgcttc gcaattctat caagggtatc
     6961 ggaacaaccc cgcaattctc cagctgatgg tggatatctg ttatcgcgtc gcgtacgccg
     7021 acggaacgat ctcttccaag gaagaagcgc tgatcctcaa aatcgcccgc ctctttcact
     7081 tcagcgaacc cctcatcgaa acgttccaga agcggtatgg tctggcgacc acatcgttgg
     7141 agcaagccta tgcgacgctt gggctttctt ccggcgcgac caatgaggag attaaacgag
     7201 cctaccggaa gctttcgatg gagtatcatc ccgatacgct catttcaaaa gggttgggcg
     7261 aggagttcct caaagccgcc acggagaagt tccgggcgat tcaggatgca tatacccgga
     7321 ttaagcagga acgaaacctt tagaaggaaa cccttcctcg aagcgatctg gaaaccgtca
     7381 cgcggtcggc gtattccaaa tctcctccga tcggcaagcc gctggcgagg cgggtgaccg
     7441 taatggcggg gtggtctttc agcagatgac ggagatacaa cgcggtggtg tcgccttctt
     7501 ccgtcgggtt ggtcgcaatg atcacttcgc tgaaagagcc ttccctgatc cgcttcatca
     7561 acttaccgaa gctcaaccgt tccggcccga ttccatcaag gggactgatc gcccctccga
     7621 ggacatggta cagcccgtcg aacgccccgc tggcctgaag ggtgagcaca tcctgcggct
     7681 cctccaccac gcagatgatc gtttgctccc ggttgggatc gctgcaaatc gagcaggggt
     7741 cggtttcggt gtagttcccg cacaccgaac acggcttgat ccgctcttgg atcgtggcga
     7801 tctcctcggc gaggttccta ttgtaggaag ggtcgctctt cagcaggtgg taggcgaggc
     7861 gggtggcgct cttcggtccg atgcccggca gacggctgag gtctttgatg agggtgtcca
     7921 gactcgtcat gattgcccca tcatcgagac ggctttcagg ccttcgtcct gaagcttctg
     7981 ctgaagcttt gccgatgcat cgttgaacgc gctctgaatg agcagctgca gagtttcaag
     8041 atcctcggcg gccgcttctt caatggcgat atccacaacg gtgaacttgc cgttgagtgt
     8101 gatcgtgacc attcctgccc cggcattgcc ggtcgcccgg atcgcaggca ggatatcctg
     8161 cattctggcc atctgttctt taatcgcctg agcgttcttg agcaattcaa acgggttcat
     8221 cactgagact tccttgtgag cacatcaccg cggaacacgg tggcaatatt tcgtaatacg
     8281 gggtcctccc cggaagggct ttcttcactg gctttcgacg tctccccatt gatgcagtac
     8341 acatcgacgc cgggccccgt caactcctcg atcgctttca ccaggcgttc gctgttttcc
     8401 ttcgctgtcg tcgctgcgaa tgcggtattg aacgtgaagg taagcgttcg atcattctgg
     8461 ttcagttctt tcaccgttcc caagacccgg gcaagcatcg gagcgtctcc gaggcgccga
     8521 gtgagctcgg gcagatcctc catcgtcaag gtccggcttg gccggcttgg aggggcagcg
     8581 ggggtaatac gttcctgctg cttgggaggc ggagcgggag cctggacttt tcgggtgacg
     8641 tgcacatttc cggaaagaag ctcctcacgc aaggtggcga tctcctttac caaggcggtg
     8701 ttggagcgaa ggctcttcag ttctccgagg cggctgatgg ccagttcaag ctcgatccgc
     8761 ggattcagcg aatagcggat ttcccggtat gtggcaagaa gcagctccaa ggcggcttcc
     8821 agctgctccg tcgtataggc ggtccgcaaa gcgaggggaa ctcgatcgct ttgaactccc
     8881 aaaatcgctt cgtcgttgat ttgtgagcgc atgaatagga ggcttcggta gaactgagtg
     8941 agatccttga ttgtttgttc gacggagacc cccgcgttga tgagggagtg gatggcaagc
     9001 agggcagttt gggtgtttcc ttcaagaaga tcggtggcga tggtgatgat tctatcaaat
     9061 ccgacgagcc cgagcttgga ccggatcgtc tccatcgtga tgtgtcccgc actgaaggcg
     9121 acgacctggt cgaaaagggt gtaggcgtcc cgcatcgagc cggtggcctc tcgggcgatc
     9181 cagaagaggg catcgtcatc ggcttggatg tcctgatcga cagccgcttg ggcaaggctc
     9241 tccttgatga gatccagacc gatgagctgg aagtggaact gctggcagcg ggagcggatc
     9301 gtcgccggca ctttctgcag ctcggtggtg gcgagaatga agatcacgta ggggggcggt
     9361 tcctccaagg ttttgagcag cgcgttgaac gcgctgatcg agagcatatg gacttcgtcg
     9421 atgatgtaga tcttgtagcg gcctgtctga ggcggaaaga gaacctcatc cttgatctgg
     9481 cggatgtcgt tgactccggt attgctggct ccgtcgattt caatcacatc gacgctgttg
     9541 ccggctttga tttcgataca gtggctgcat accccgcacg gtttggtggt gggaccttcg
     9601 tcacagttaa gggaacgggc gaggattcgg gcagcggatg tttttcccac tccgcgggga
     9661 ccgctgaaga gatacgcatg ggcgattcgc ccttgggcga tcgctccttc gatcgtggag
     9721 acgacgaacg gctgaccgac aagagtggaa aactcctgcg gccgtttctt cgttgcggtt
     9781 acttcatact gtgacatctg ccccccaaag ttcgttgaca ctttaccata gatgaagggg
     9841 ttttaataga accagccaac aaatcgcctg cgtctcacaa gcggtccact taccgctgct
     9901 cccttccggg cctgacgggg ttgggcggat gcctgtagca cagaatctgt tggctggaaa
     9961 aaaacggaga gaaggagatt cgaactcctg gtgcttgcgc acacacgctt tccaagcgtg
    10021 caccttcgac cactcggaca tctctcccgg tgaccaataa aacgttcgga gagagaggga
    10081 ttcgaacccc cggagacttg cgccccaaca gttttcaaga ctgcctcctt cgaccgctcg
    10141 gacatctctc caaatatttt atgctatgag accaagagga ttcgaacctc cggccctcag
    10201 ttccgcaaac tgatgctcta tccagctgag ctatggtctc gctataaaaa aacaaaatcg
    10261 gagagggggg gattcgaacc cccggacccg ttaccaggtc aactccttag cagggagccc
    10321 gattcggcca ctctcgcacc tctccttgaa caaaccggct tcttggcaac tgtagccttt
    10381 cggagagaga gggatttgaa ccctcggtac tggagaccag tacaacggat ttcgagtccg
    10441 cccccttcgg ccgctcgggc acctctcctt gtttggggcc ctgagcagag ctcagtaacc
    10501 aatcggattc aaactatagt cagtggtatc atatttgtca agaagggttt ggtcgacacg
    10561 atcgggcaat cgtgttcatt cccatcaaca tgaccctgtc tttcatagga gggatcttgc
    10621 tttaataacg acgaatatat ttgacatact aaccattgta tgcataatat attggatgta
    10681 atggcgaaac gaacaccggt attgctccca tgggtggaac gaatcctcat tgatctcggg
    10741 gaacggatcc gtcttgcccg gctcaggcgg aatatcagcg cgcagctgct tgccgaccgg
    10801 gcgggcatca gcaggcagac cttgagcacc ttggaaaacg gctccggctc ggtatcgctg
    10861 gccacgttca ttcaggtgct cttcgtactc ggactggaaa aggatctcga agaggtcgca
    10921 aaagacgatg tgctcggaag aaagctccag gacttgaacc ttcctacccg aagacgggcg
    10981 acaaagcgcg accgataggc ggtagaaaat ccatcgaatc ctctgtaagg atatcgggaa
    11041 tcgggatcga tccgtttagt tgacagattt ccaccacctc gtcatcatac gaggtatggg
    11101 gaagaacgag gcgcctccct cttcgaggaa gaaaacgaag cttgcgacat tcaaggccgg
    11161 gcggcttgcc cagcgcgctt tggcgttcac caaggaggtg cttgctcaat cggggccgcg
    11221 caagagcggg agtctcgctg cgcttgaagc tgcggaattg atcaagagcg agcttgaaac
    11281 cacctgcgac accgttgaaa gccaacaggt cccgatcgac tcgaagatca acacccggct
    11341 gttcaccctc gtcgtgtata cctatccggc ggtcgttctg ctctgtctcg tcggtcttcc
    11401 ctatctcggg cttctgctct ccgccttggt gctctggttc agcttcgaga ccttcggtcg
    11461 ctataaaccc cgatatcttg ccccaacgga catgggggtg aacgtccatg ggatcattga
    11521 acccgtgaaa gctgttgaat cgacgatcat cttcaccgcc caccacgaca gcgccccgat
    11581 cgagcgcttc gagaagaaga aagcgatgat tccattcggg ctggtcggtg gagccgcact
    11641 tctgtctttt ctccaaacgg ccgtcgaatt gactcagctt cgcctgtttc gacccaattt
    11701 tccgccgatt ctgctgttcc ttctcggcct tggacttctt ggcggactct attacgtgcg
    11761 aacgctcttg gagtattaca ccgatgaggt tgcccccgga gcgggagaca acctgatctc
    11821 ggtggggata cttgccgcgc tcgcccaata tttcaaggcc gaaccccttt ccaaaacccg
    11881 gataatcctc gcttcctttg acggcgaaga gatttttctt cagggctcga cggcgtggta
    11941 tgaagcccac aaggaagcgc ttgaaggtgc agtcgtcctg aactttgatt cgatcttcag
    12001 cgccgaacac cttgtctttc tggaacgtga tgcgaacgcg acgctcccgc tgtcgaaatc
    12061 gttagctcgc aaatgcgtca acatcgcccg ctcgatgggc tatgaagcat cagcgttgtc
    12121 gatgccgatt ctcggcgggg cgacggacgc cgcttcggca gctcggatcg ggctggaagc
    12181 gaccaccttg accgcctcct cgtggagtga cggcgtctac cacaccaaag atgatacctt
    12241 ggcggccatc gatcctgaga gtgtggagcg atcgatcggc atcgcgatcc gccttgcccg
    12301 gctgctcgat caggacagcc tgtgggatga cgaccttccg atcgaagagg agaaggtcga
    12361 ggagccgacg aatccaacgc tcgtcttctc gaagctcact cgacgatgag gctcgccccg
    12421 ccgttggtga ggttccgtac gtacagattc aggtcttcta tatcggtggc gggaagggtg
    12481 gcggtgatcg tcacatccgt ggtgaactcc tcgctttcga tctccgcttc caaagaaaca
    12541 agctgcaact ttaggagttc atagaggttg tagggcaccg tcacccggaa tgcggtcttc
    12601 tcgatgagct cttcggtctc caacagcgga agcagttcgc ggacgctctg accatacgcc
    12661 ttaaccagac ctccggttcc cagcaggatt cccccgaagt aacggatgat gagaatcagg
    12721 atgttggtga tgccgctacc cttgagaacc tcgaaggcgg ggcggccggc ggtgttcctc
    12781 ggttccctgt catcgctgaa gctgtacaga tcgcctttcg gcccgaccac cgcggcgtgg
    12841 acgacgtgca ccgcctgggg atgctcttcc cgggctttct ggacgaggcc tttgatctga
    12901 tccagcgacg tgatcggaat cgcatgggcg atgaagcggg acttccgcac ctcgatctca
    12961 acggtggctt cttttttaag gatgagcata tcttctgtag gatactccat cctcacggag
    13021 ttttgaaggt tatccgcttg tcgaccatcg tctgtccgtc aaagatgaag aacccttcga
    13081 agtgcttccc ctccagagcg ggcggcagcc agtacaggtc atcctcccac atccggtcat
    13141 aggggatctc ggagatcggg acccagaacg gccgggcttc atcggtttcg gcgagttcgc
    13201 ccgtgtaggt ccgagtgaag aacacgtacg ctttttccgc aaggccgtcg cggaactgga
    13261 agttcaggac tccgaccatc tccagatcgc tgatgtcgag gcgggtctct tccttgaact
    13321 cccggatcgc cgcctccatc gccgtctcat cctcttcgat gtgtccgccc ggagcgttga
    13381 ccagtccccg gccgaggccg cgcttcttgt cgatgaggag aacccgatcg tcatcgaaga
    13441 gataggtgat tacgcagatc tcactcggtt cccagatctc ccagtcgatc tgttcgacgg
    13501 attcgacgtc ctggacgtcg atcgtcacct cgatcggatc ctcttgttcg gcctgttgcg
    13561 gatgggcgat ttcccagcat gtcttacaga ggttctcatc cccgccgatg acgtggttga
    13621 aatcaagctt tgcggagcat tcgacgcagc gcagcttgaa cggccaggcg cgcttgcgca
    13681 gcagaaagat gatgaggacg cacaacagcg caagcggaat ggcgaggtaa tggctccatc
    13741 cctttgtcag gatcgtgatg agcccgagct gaaagaggag aagaaccgag gcgatataaa
    13801 gcgttgcggt tcgtttctta tggctgttgg gaatttttcg ctgagtcagg ttcagcaaaa
    13861 gaattccgat gagagcccat gagtaaaagc gttcaatcgt ttcaagaatc atagaggtag
    13921 ggtattggca gcggg
//
LOCUS       DAOSLI010000044        13221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_4759_length_13221_cov_23.415692, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000044 DAOSLI010000000
VERSION     DAOSLI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4759_length_13221_cov_23.415692"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..74
                     /locus_tag="PLF67_06945"
     tRNA            1..74
                     /locus_tag="PLF67_06945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Arg,seq:tct)
     gene            complement(132..794)
                     /locus_tag="PLF67_06950"
     CDS             complement(132..794)
                     /locus_tag="PLF67_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89780.1"
                     /translation="MKKKAGIVAIGIVLLLVACSGSISSELYIRDLLDLAASPNQVFY
                     TQGTVSVESFGSDSNEDLKEMIVTWFRDAQNFRETTIDFSTYLVADIKIPIAYADNEL
                     LKKEQDLLSIVVKKDKLRELEFGIRLDKNVFAKIQEHVREMFYQDLSISDWTFSIDLK
                     NDTRNAQSMTLQTIYANDEPLLYPKTITVKERETVKLSFPDILRDYSYLYGDVFFGTL
                     AL"
     gene            1235..2368
                     /locus_tag="PLF67_06955"
     CDS             1235..2368
                     /locus_tag="PLF67_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89781.1"
                     /translation="MKGVEVTVDRVTRSYGDFKALDDVSVTIKRGEFFSLLGPSGCGK
                     TTLLRIIAGFDYPDRGSVLFDNKDILPIPPDKRQSNTIFQTYALFPHLTVYENIAFPL
                     RLRKLGKEEIDKRVKQYVHMVQLDDHVYKKPSMLSGGQKQRVAIARALINEPSVLLLD
                     EPLSALDAKLRQNLLVELDDIHDKVGITFIYVTHDQSEALGVSDRIAVMDKGKILQIG
                     TPYEIYEAPATRFVAQFIGETNIFDCTVLSCEPFENEYMIVVDVPALGTTINVTDLEP
                     QEIGSKFDFTVRPEKIRITLNPPRSTAAGLNVFKGVVEEPVYSGFQSKYYVRLDDNTT
                     APLMKAFKQHTAFLEDGPDIEWKDSVYLSWTAVDGYLVKDVDR"
     gene            2365..3291
                     /locus_tag="PLF67_06960"
     CDS             2365..3291
                     /locus_tag="PLF67_06960"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89782.1"
                     /translation="MKKAIKPAKREQLLGTLYSFPMGIWFTVFFTVPLAIIILYSFMK
                     RGLYGGVEKAFTLEAYRQMINPSFAKVLVRTLWISAFSTVLCVLIAIPVGYAMAKSKH
                     QTLLLFLIIIPFWTNSLIRIYAWISILSTEGFLNSLLIRLGIVKEGLHLIYNNGSVIL
                     VLVYMYLPFAILPLFTTIDKFDFFLLDAARDLGATKLEAIVKVMFPNIKSGLSTAFIF
                     TFIPIFGAYTVPLLVGGKDSTMIGNIIVDQVSKTRNWPLASAFSMILTVASLIGVFWM
                     LASAQREAAGKNKNTPQAYEQKVKAHHGRSKR"
     gene            3288..4091
                     /locus_tag="PLF67_06965"
     CDS             3288..4091
                     /locus_tag="PLF67_06965"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89783.1"
                     /translation="MNYRPYSKKKHLSFSNTMLGLTILFLFIPLFVVTFFSFNSAKGM
                     QWEHASLIWYKTLIFDSPSLWLAFKNSIIIALTSSVTATVLGSLAAIGITWYRFKGRS
                     YITTISYLPMVLPEVIIGISMLIFFSSVKIKLGMFTIFVAHATFNLPFVFMMVQARLD
                     EFDYSTIEAARDLGANERQALVKVVVPAIMPAILSSFLMCTTMSLEDFVITFFVSGPG
                     SGTLPLYVYSMIRYGVSPVINALSFVMILGTMLIAFTFRRFLKTAAASA"
     gene            4122..5180
                     /locus_tag="PLF67_06970"
     CDS             4122..5180
                     /locus_tag="PLF67_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89784.1"
                     /translation="MKHIRLFFIVLLIGLLSFTLISCNRSSKSAEKLYLYNWTYYTPD
                     ELVAKFKKETGIEIIIDNFASNEEMFAKVMAGGAEGYDIIFPSSDYTSIMINHDLVQE
                     LDHSLLPNLKYISPTVTEKADYDPNLKYHVPYFMGSSGIAVNTSRVPFEYERTWAIFA
                     DQRLAGRMTFLDDMREVMGAALKHLGYSGNTTDERELDEATALIINEWKPNIIKFDSE
                     SFGKAFSRGEFWVVHSYPENVFAELPADMWESVDFFIPPEGGMMYIDNMVIPKGAKNV
                     ENAHKFMNFIHDPKNYAIFLDEFDFPPTTNTGAEKYMKNDAFFFTDKDLANSDLNDDL
                     GEHLDLYNIRWQKIRYEH"
     gene            5492..6772
                     /locus_tag="PLF67_06975"
     CDS             5492..6772
                     /locus_tag="PLF67_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOE89785.1"
                     /translation="MNNYHFETKALHAGQEQPESATGSRAVPIYQSTSFVFDDSADAE
                     ERFALRRSGHIYTRLNNPTQDAFEARIAALEGGVGALAVASGMAAINYAILALAKQGD
                     HIVSAKTIYGGTYNLFAHTFVEWGISTTFVDPGNLKEVTEAIGANTKALFIETIGNPN
                     ATLCDIGALAEIAHDHKIPLIVDNTFASPYLLRPIEHGADIVVHSATKFLGGHGTSLG
                     GVIVDGGTFDWQASGKFPSLVEPNPSYHGFSFTEQAGNAAYITKIRTLLLRDTGSAIA
                     PLNAWLFLLGLETLALRVQRHGENTAKVIAYLQNHPKVRKINHPCIPTHPQHHLYQRY
                     FPNGGVSIFTIELVGGGETARSFIDSLKLFSLLANVADMKSLVIHPASTTHAQLSDAE
                     KAEQGITESTIRLSIGCEHPDDIISDLEQALGGV"
     gene            6772..7680
                     /gene="metA"
                     /locus_tag="PLF67_06980"
     CDS             6772..7680
                     /gene="metA"
                     /locus_tag="PLF67_06980"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOE89786.1"
                     /translation="MPVKVTYPVDANSSFFFMDEERARTQDIRPLKVLILNLMPNKSV
                     TEQQLLRLLGNTALQVEVSFLTTATYQSKNTPKSYLKTFYQTFDQIRNSRYDALMITG
                     SPVEHMAFEDVAYWAELVEILDWANENVFSSYFICWAAQAALYHYYGIGKRPLNKKLS
                     GVYRHRVVEPAHTLIRGFDDLFWAPHSRHTTIDEEAIGKEEDLIVLAESEEAGTYLLV
                     GSGGRRVFVTGHGEYDALTLDSEYRRDTQAGLPVEEPKNYYEGGSVTVRWRAHANLLV
                     SNWLNYHVYQTTPYDLAQLSRQDLQS"
     gene            complement(7650..8348)
                     /locus_tag="PLF67_06985"
     CDS             complement(7650..8348)
                     /locus_tag="PLF67_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOE89787.1"
                     /translation="MKAIIGGTGFDRLEGLSLKKRLVETPYGGVDLFLGQGEDEGLVF
                     LPRHGSSHSVPPHRINYRAHALALKQIGVVEAIAIYAVGSIDDTLKPGEVGIVSDFVD
                     HTGRSQEHTLFDRPVTHIEVDKPFDEELKKRMLALKPTLLDAGVYVTTNGPRLETKAE
                     IAFFKRVGFSVVGMTLASEVTVLLEAGIRTLALAYSINWAAGVLEEFSFLPDSQLERL
                     KTEVTQLCRSCLLS"
     gene            complement(8345..9358)
                     /locus_tag="PLF67_06990"
     CDS             complement(8345..9358)
                     /locus_tag="PLF67_06990"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOE89788.1"
                     /translation="MVHLPFLLTEDGICRLQEGHLFILDRRRYPFERVEVSCRNAEEV
                     ATALKAMVTQGGGPLEVALKTMIMEARNHERLPLLNQVRHLEEAARLLADARPTNTTM
                     ARSLDEVLSEVEQRKDEPPVKVVEEAVRTILDRFAGIYHRIGKLGSTLIEDGDQILTT
                     CFAEHTFLLSLLYAKQAGKNIRVLVSETRPYLQGSRLTAPSLEEAGIPLRVITDSMGP
                     TMIASGKVQLYMTAADLVCRDGTVVNKIGTLANALACSRYGIPYHVLAISPDPNKKTR
                     ADVTIEMRDPKEVTECMGIKTTLDTIEGVYPAFDIVDPELVTRIITPKGILRPGEMEE
                     DGI"
     gene            9484..10725
                     /locus_tag="PLF67_06995"
     CDS             9484..10725
                     /locus_tag="PLF67_06995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOE89789.1"
                     /translation="MYRKQLRTIILMLLIVSIVSGSLLFAAGKKEAAPNSFLVRVINV
                     LSPEGTPAFSVKDDDGSEHLLYTGIFTDTNFPIEDIAVGDYLEVVVDNSIIHSMRYVS
                     PIIEENRIPLPSPMDEMSLIDRFSYAYGYQLMQMFAQQGLFLDPNYWARGSVDGIHDM
                     TNPDSIGFFEFTDLFGIIDQFQKEIWEVGEAPTSYPDALSLEEVLNLTADDDLFKKFS
                     YTYGYLLVSDLLKLALDANAKLYAQGGLDFAGSNDPLIDEFGMQMTFYEYQMILEEQY
                     QAEMAALAQRNLEEAETFLAMNTKKEGVVTTESGLQYTILEEGSGPKPTADDQVEVHY
                     TLYLLNGQLVESSKDSGQSVHFSAGNLIEGFTEALLTMNVGSTIRTWIHPSLGYGEYG
                     SQAIEPNALLIFEIELLGIDE"
     gene            complement(10778..11179)
                     /locus_tag="PLF67_07000"
     CDS             complement(10778..11179)
                     /locus_tag="PLF67_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89790.1"
                     /translation="MAEVESPLMLIDLLDRLEWYRGTHPSWQRVIDILDRSLPYDDPD
                     GEHFVDSVRYQVAAYRTTEEGSVAVAEENRLLVLLEGEELFSLSEGKEVLLSTVFTEG
                     LFIYLRKGERYKSKQTYSQEKIVRRVVFFLP"
     gene            complement(11155..11790)
                     /locus_tag="PLF67_07005"
     CDS             complement(11155..11790)
                     /locus_tag="PLF67_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOE89791.1"
                     /translation="MSTNTVFTIGRQYGSGGTEIGRRVAKELDIPFYDKELIAISARE
                     SGLSESLFTNADEKATSSIFYSLVMGNYPMASGALGVTEMPLNDQLFLIQSKTIKRLA
                     GEGPCVIVGRCADYILRDMENTLNIFIHADLAYRVERAITMYEAPANKAEDICIKTDK
                     QRANFYNYYSDRKWGRCRTYDLAINSAKLGIEGSALHIIHFDEEWQKSKVR"
     gene            complement(11852..12625)
                     /locus_tag="PLF67_07010"
     CDS             complement(11852..12625)
                     /locus_tag="PLF67_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOE89792.1"
                     /translation="MLRLLMFNLEATLFSGQTFAWQKDGSTYRGVIGRHLVSLDASDP
                     FSTIGSDPFLRRYFDLEYPYEEAEAHLEILDPMLGELLLAHRSIHILNQDPWETTLSF
                     ITSQNNTIKRIRTLLNTLRQTYGKEVAPNHHALPTPDELLRGSSEEKLRALGFGYRAE
                     YLLDAARKSNLLATVSDLADEEALQTLRQIHGVGPKVASCILLFAYGRKDVFPIDTWT
                     GRILAHHYPGKDPSFFSPYGGIAQQHLFYAERRKIGSHW"
BASE COUNT         3148 a         3565 c         3319 g         3189 t
ORIGIN      
        1 gcgccaatag ctcagtagga tagagcaact gccttctaag cagtaggtca gaggttcgag
       61 acctctttgg cgtgctactc gttccaactc ctataaagtt tgttcattct ttataggagt
      121 tgttcttttg tctacaacgc aagtgtgccg aaaaacacat ctccataaag atagctgtaa
      181 tccctcagga tatcagggaa actcaatttc acggtttccc gttcttttac agtgattgtc
      241 tttggataca gcagcggttc atcgtttgcg taaatcgttt gaagagtcat cgattgagca
      301 ttccgggtat catttttcag atcgatgctg aacgtccaat cactgatgga gaggtcttgg
      361 taaaacatct ccctcacatg ctcctggatt ttggcaaaaa catttttatc caaccgtatc
      421 ccgaattcca attccctgag tttatctttt ttcactacga tactcagcag atcttgctct
      481 ttcttcagca gttcattatc ggcataagct atcggtattt taatatcggc aacaagatag
      541 gtgctaaaat caatggttgt ttccctgaaa ttctgagcat ctctgaacca agtgacgatc
      601 atctctttaa ggtcttcatt gctgtcacta ccgaaggatt caacactgac ggttccctga
      661 gtgtaaaaca cctgattcgg acttgccgcc aaatccagca aatcccggat gtagagttca
      721 gaggaaatgc ttccggaaca ggcaaccaga agcagaacga ttccgattgc tacgatacct
      781 gccttctttt tcatggcccc ctccctgacg tggtttgatg aggacagaat agcacacgtc
      841 accatgattt tggggtcttt tttcaattgt ttacgagcca agaagttaat gagagtcggt
      901 aagacctttt ccactgcatc tcattccatc aaacccactg cgttgaacga tctcactggg
      961 tatcaatcag atctcttttg gcgtttgtct ttaagcgcgg tgagtgtatc atttacagca
     1021 accgcccttt aaactgtaag ctggagcttc gagacctgtt tggggtgaaa aacccccatt
     1081 ttcttgacaa gaaagctttt attatttata tataaaagaa cgagttataa aacaaatgac
     1141 aaagatgccc cctctatctt gacaaaaggg ggtggataat tgatattcgt tgtacacgca
     1201 cacgacaaaa cggcaaggat gggaatgaaa gagattgaaa ggtgttgaag ttacggttga
     1261 tcgggtaacc cggtcctatg gtgatttcaa ggcactcgac gatgtgagtg tcaccatcaa
     1321 gcgaggagag tttttctctc ttctgggtcc ttcggggtgt ggtaaaacca cccttttgag
     1381 gattatcgcc gggtttgatt acccggatcg tgggagcgtg ctgtttgaca ataaggacat
     1441 cctcccgatt cccccggaca agcggcagtc caatacgatt ttccagacct acgcgctgtt
     1501 tcctcatttg acggtatatg aaaacatcgc ctttcccctg cgcctgcgca agctcggaaa
     1561 agaagagatc gacaagcggg taaagcagta cgttcatatg gtccaattgg acgatcatgt
     1621 ctacaagaaa ccctcgatgc tcagcggcgg acagaagcag cgcgtcgcga tcgcccgagc
     1681 cctgatcaat gaaccgagcg ttctgttgct cgacgagccg ctctccgccc ttgatgcgaa
     1741 gctgcggcag aacctcctcg ttgaactgga cgacatccat gataaagtag gaatcacctt
     1801 catctatgtc acccacgacc aaagcgaggc gctcggcgtc agcgaccgca tcgcggtgat
     1861 ggacaaaggg aagattcttc agatcggcac cccctatgaa atctatgaag cgcccgccac
     1921 ccgcttcgtc gcgcagttca tcggcgagac caatattttc gactgcacgg ttctctcctg
     1981 cgagcccttc gaaaacgagt acatgatcgt agtcgatgtt cccgccttgg gcaccacgat
     2041 caacgtaacc gatctcgagc cgcaggaaat cggttcaaaa ttcgatttca ccgttcggcc
     2101 ggagaagatc cgcatcacgc tcaatccgcc gcgctcgacc gcagcgggac tgaacgtctt
     2161 caaaggagtc gttgaggaac cggtatattc agggttccaa tcaaagtact acgtgcggtt
     2221 ggacgataat accacagcgc ccctgatgaa ggcgttcaag cagcacacgg cgtttttgga
     2281 ggatgggccg gatatcgaat ggaaggattc cgtctatttg agctggaccg ccgttgacgg
     2341 atatttggta aaggatgtcg atcgatgaag aaggcaataa aacccgccaa gcgcgagcag
     2401 cttttaggaa cgctgtacag ttttccgatg ggcatctggt ttaccgtctt cttcaccgta
     2461 ccgcttgcga ttatcatcct ctactccttc atgaagcgcg gcctctacgg tggagtggaa
     2521 aaggccttca cgcttgaagc gtacaggcag atgatcaacc cgtcctttgc gaaggtgttg
     2581 gttcggacgc tgtggatcag tgcgttctcg acggttctct gcgtcctgat cgccataccg
     2641 gtcggatatg cgatggccaa aagcaaacat cagaccctcc tgctcttctt gatcatcatt
     2701 ccgttttgga cgaacagcct gattcggatc tatgcgtgga tcagcattct ctcgactgaa
     2761 ggatttttga actccctcct catccgcctc ggcatcgtga aagagggact tcatctcatc
     2821 tacaacaacg gcagcgtgat tctggttctg gtatacatgt accttccgtt tgcgatcctc
     2881 ccccttttca ccacgatcga caagttcgac ttcttcctcc ttgatgccgc ccgggatctc
     2941 ggagccacga agctggaagc aatcgtcaaa gttatgttcc ccaatatcaa aagcgggctc
     3001 tccaccgcct tcatctttac cttcattccg atcttcgggg cctataccgt ccccttgctc
     3061 gttggcggca aggattcgac gatgatcggg aatatcatcg tcgaccaggt atcgaagacc
     3121 cggaactggc ctttggcaag cgcgttttcg atgatcctca ccgtcgcctc gttgatcgga
     3181 gtcttctgga tgctcgcctc ggctcaaagg gaagcagccg gtaaaaacaa gaatactccg
     3241 caagcgtacg agcaaaaggt caaagcccac cacgggagga gcaagcgatg aactaccgac
     3301 cctactccaa gaaaaagcac ctctcctttt ccaacacgat gctgggtttg acgattctct
     3361 ttctcttcat tccccttttc gtggttacct tcttttcctt caactcggcg aaggggatgc
     3421 agtgggagca tgcttcgctc atctggtaca agaccctgat cttcgacagc ccctctctgt
     3481 ggctcgcctt caagaactcg atcatcatcg ccctgacgag cagcgtcacc gccaccgtcc
     3541 tcggatcgct ggcggcgatc gggatcacct ggtaccgttt caagggccgt tcatacatca
     3601 cgacgatcag ctacctgccg atggtcttgc ccgaggtcat catcggtatc tcgatgctga
     3661 tcttcttctc ctcggtgaaa atcaaactgg ggatgttcac gatttttgtc gcccacgcaa
     3721 cattcaacct gccgttcgtc ttcatgatgg ttcaagcccg ccttgacgag ttcgattact
     3781 cgacgatcga ggcggcccgg gacctgggag cgaatgaacg ccaggcgttg gtgaaggtcg
     3841 tcgtcccggc gatcatgccg gcgattctct caagttttct gatgtgcacg acgatgagct
     3901 tggaagactt cgtcattacg ttcttcgttt cgggtcccgg ctcgggaacc ctgccgttgt
     3961 acgtctactc gatgattcga tatggagtgt ctccggtaat caacgcactg agttttgtga
     4021 tgattctcgg tacgatgctc atcgccttta cgttccgtcg cttcttaaag accgcagctg
     4081 ccagtgcctg acgctgctct cgacctatgg aggtttacac catgaaacac attcgtcttt
     4141 tctttattgt tctgctcatc gggcttctaa gcttcaccct gatctcgtgt aatcgttcaa
     4201 gcaagagtgc cgaaaagctc tacttataca attggaccta ttatactccc gatgaactcg
     4261 tggcgaagtt caaaaaggaa acgggaatcg agatcatcat cgacaacttc gcttccaacg
     4321 aagagatgtt cgccaaagtg atggccggtg gtgctgaagg atacgatatc atcttcccct
     4381 ccagcgacta cacctcgatc atgatcaacc atgacttggt tcaggagctt gaccactccc
     4441 tcttgccgaa cctcaaatac atctccccca ccgttacgga aaaagcggat tacgatccca
     4501 acctcaaata ccatgttccc tacttcatgg gttccagcgg aatcgccgtc aacacttccc
     4561 gtgttccctt tgaatacgag cgcacttggg cgatcttcgc cgatcagcgc ctggcgggaa
     4621 gaatgacttt tctggatgac atgcgcgagg tgatgggagc ggcgttgaag cacctcgggt
     4681 acagtggaaa cacgaccgat gaacgggaat tggatgaagc caccgccctg atcatcaacg
     4741 agtggaaacc gaatatcatc aaattcgact cggaatcctt tggaaaggcg ttcagccgcg
     4801 gcgagttctg ggttgtccat tcctacccgg aaaacgtctt cgccgagctg cctgccgata
     4861 tgtgggagag cgtcgacttc ttcattcccc ccgagggcgg catgatgtac atcgacaaca
     4921 tggtcatccc caagggagcg aaaaacgtgg aaaacgccca caagttcatg aacttcatcc
     4981 atgacccgaa aaactatgcg atcttcctcg acgagttcga tttcccgccg acgacgaata
     5041 cgggagccga gaagtacatg aagaacgatg ccttcttctt taccgacaag gacttggcca
     5101 acagcgacct caacgatgat ctcggtgaac acctggatct ctacaacatt cgctggcaga
     5161 agatccggta tgaacattag tctgtgactc ccctcaaata cccttacgat cgcccgtaag
     5221 ggtattttta catgcatgca gttgacaggc aggcaccaag cggatagact ctgcgtaatc
     5281 cgggcaggtc gcccgggcat cttcttggag gaggcacatc atgatgcgaa gcaatgacag
     5341 ttcagacccc tgcatgtgcc gcatggcatt gtaaccactc tccaaggagc tttccatgaa
     5401 acatcacata catcctcgtt gcggctctgt tgccgatcac atgtgacttc cttcggtctt
     5461 tatcatcact acaccaaaga aggaaaaaca tatgaacaac taccattttg aaacaaaagc
     5521 attacatgca ggccaggaac aaccggaatc ggcgaccggc tcgcgggcgg tgccgatcta
     5581 ccaatccacc tcgttcgtct ttgacgattc tgccgacgcc gaggagcgct tcgccctcag
     5641 gcgctcggga cacatctaca cccggctgaa caatccgacc caggacgcgt tcgaggcacg
     5701 gattgcggca ttggaggggg gcgtcggagc gttggcggtt gccagtggaa tggcggcaat
     5761 caattacgcg attctggcgc ttgcgaaaca gggcgaccac atcgtctcgg cgaagacgat
     5821 ctacggcgga acctacaacc tcttcgctca caccttcgtc gagtggggaa tctccacgac
     5881 gttcgtcgat cccggcaatc tgaaggaagt tacggaggcg atcggagcga acaccaaagc
     5941 gctcttcatt gaaacgatcg gcaatcccaa tgcaacgctc tgtgacatcg gggccttggc
     6001 cgagatagcc catgaccata agattccgct catcgtcgac aatacattcg cctccccata
     6061 cctgcttagg ccgatcgaac acggggcgga tatcgtggtg cactcggcaa cgaagtttct
     6121 cggcggtcat ggaacctctc tgggcggggt catcgtcgac ggaggaacgt tcgattggca
     6181 agcgagcggc aaatttcctt cccttgtcga gcccaacccc tcctaccacg gcttttcgtt
     6241 caccgagcag gcgggaaacg ctgcatatat caccaagatc cggaccctcc tgcttcggga
     6301 taccggttcg gcgatcgccc cgctgaacgc ctggctcttc cttctgggac tggaaacgtt
     6361 ggctcttagg gttcagcgcc atggcgagaa caccgccaag gtgattgcgt atttacagaa
     6421 ccatccgaag gtgcggaaga tcaatcaccc atgcattccc acccaccctc agcaccacct
     6481 ctatcagcgc tatttcccca atggcggagt ttccatcttc acgatagaat tggtcggtgg
     6541 aggtgaaaca gcccgctcct tcatcgattc gctgaagctc ttcagtctgc tggcgaacgt
     6601 ggccgatatg aagagccttg tgatccatcc cgcctccacg actcacgccc agctgagtga
     6661 tgcggaaaaa gcggagcagg gaattacgga aagcacgatc cggctgtcga tcgggtgtga
     6721 acaccccgat gacatcatca gcgaccttga acaagcgctg gggggtgtgt gatgccggta
     6781 aaagtaacct accccgtcga tgcgaactcc tcgttcttct tcatggatga agagcgggcg
     6841 cgaacccagg atatccgccc cctcaaggtc ctgatcctca atctgatgcc caataagtcg
     6901 gtaacggagc aacagctcct tcggctcttg gggaacacgg cccttcaggt cgaggtgagt
     6961 tttttgacga cggcgaccta tcaatcgaaa aataccccga agtcctacct gaagacgttc
     7021 tatcagacgt ttgatcaaat cagaaactca cggtacgacg cgttgatgat caccggcagc
     7081 cccgtcgaac atatggcctt tgaagatgtt gcctattggg cggagctggt tgaaatcctc
     7141 gattgggcga atgagaacgt cttctcctcg tatttcatct gctgggcggc tcaggcggcc
     7201 ctctaccact attatggaat cggtaaacga ccgctgaaca aaaagctcag cggcgtatac
     7261 cgccatcgag tggtggaacc tgcccatacc ctgatccgcg gctttgacga cctcttctgg
     7321 gctccgcaca gccggcacac gacgatcgat gaagaggcga tcggaaaaga ggaagatctc
     7381 attgtgctcg ctgaaagcga agaagcggga acatacctcc tggttggcag cggcgggcgg
     7441 cgcgtgttcg tcaccggtca cggcgaatat gacgcgctta ccctcgatag cgagtaccgg
     7501 cgggacacgc aagcgggact tccggtggag gaaccgaaga actactatga aggcggttcc
     7561 gtcaccgtcc gctggcgcgc ccacgccaat ctgcttgttt caaactggct caactaccac
     7621 gtctatcaga cgaccccgta cgatcttgct caactgagca ggcaggacct gcaaagctga
     7681 gtgacctccg ttttgagcct ctccaattgg ctgtcgggca ggaaggaaaa ctcttcaagc
     7741 accccggcgg cccagttgat cgagtacgcc agagcaagtg tccttattcc cgcctccaaa
     7801 agcaccgtca cttcgcttgc aagggtcatc ccgacaaccg agaagccgac gcgtttgaag
     7861 aacgcgatct ccgccttcgt ctccaagcgg gggccgtttg tcgtgacata gacgccggca
     7921 tccagcagtg tgggtttcaa ggcgagcatc cgctttttca gttcctcgtc aaacggcttg
     7981 tcgacttcga tgtgggtgac cggccggtca aagagggtat gctcctggct gcgcccggta
     8041 tggtcgacga aatcgctgac gatgccgacc tctcccggct tgagcgtgtc atcgatcgac
     8101 ccgaccgcat agatcgcgat cgcttcaacc accccgatct gtttcagggc gagggcgtgg
     8161 gcccgatagt tgatcctatg gggtgggacg ctgtgggaag atccgtggcg gggaagaaag
     8221 accaagcctt catcctcccc ttggccgagg aagagatcga ctcctccata gggtgtctcg
     8281 acgagcctct ttttcaagga caatccttca agccggtcaa aaccggttcc accgataatc
     8341 gctttcatat tccatcttcc tccatttcac cgggtctgag aattcctttc ggggtgatga
     8401 tccgagtgac cagttccggg tcgacgatat caaacgccgg atagactcct tcgatcgtat
     8461 caagggttgt ctttatcccc atgcactccg ttacttcctt cggatcgcgc atttctatcg
     8521 tgacatccgc ccgtgttttc ttgttcggat cgggactgat cgccagaaca tggtatggga
     8581 ttccatagcg ggaacacgcc agcgcattcg ccaacgtacc gattttatta accaccgttc
     8641 cgtcccggca caccagatcc gccgcagtca tgtagagttg aacctttccg gaggcgatca
     8701 tcgtcggccc catgctgtcg gtgatgaccc taagcgggat tcccgcctct tccaacgagg
     8761 gagcggtaag ccggctgcct tgcagatacg gtcttgtttc gctgaccagc actcggatgt
     8821 tctttcccgc ctgcttggca tagagcaggg aaagcaggaa ggtgtgttcg gcgaaacagg
     8881 tggtgaggat ctgatcgccg tcctcgatca atgttgaacc gagtttcccg atccgatggt
     8941 atattcccgc aaaacgatcg agaatcgtgc ggacagcttc ttccacaacc ttcaccggag
     9001 gttcatcctt tctctgctcc acttctgaaa gcacctcatc cagggagcgg gccatcgtcg
     9061 tattggtcgg tcgggcatcg gcaagcagac gggcagcttc ttcaagatgc cggacttggt
     9121 tcaagagtgg aagccgttca tggttgcgag cttccatgat catcgttttc aaggcgactt
     9181 ccaagggtcc gccgccttgg gtgaccatcg ctttgagggc ggttgcgacc tcttcggcat
     9241 ttcggcagga aacttcgacc cgttcaaacg gataccgtct tcgatcaaga ataaagagat
     9301 gcccttcctg tagacggcaa attccatctt cggtcagaag aaacggcagg tgaaccataa
     9361 aaaaagcgta gcataggtga agttttgtgg caattgtgtg agggtacatt gacgaggagg
     9421 cataaggttc gtacagtaaa aggaaccgtg gccgaggcca taagattttc atgaggatca
     9481 ggtatgtata gaaaacaatt gagaaccata atacttatgc tgctgattgt cagcattgtt
     9541 tccggatctt tattatttgc cgccggcaaa aaagaagccg ccccgaactc gttccttgtc
     9601 cgggtgatca atgtcttgtc tccggaagga acgccggcct tttccgtgaa ggatgatgac
     9661 gggtccgaac acctgctgta caccggaatc ttcaccgaca ccaactttcc gatcgaggat
     9721 attgctgtcg gtgattacct tgaagtcgta gtcgacaact cgatcatcca ttccatgcgg
     9781 tatgtgagcc cgatcattga agagaaccgg attcctcttc cgagtcccat ggacgagatg
     9841 agcttgatag atcgcttcag ctatgcgtac ggctaccagc tgatgcagat gttcgctcaa
     9901 cagggtctct tccttgatcc gaactattgg gctcggggaa gtgtcgacgg aattcatgat
     9961 atgacgaatc cggattcgat cggattcttc gagttcaccg atctctttgg catcatcgac
    10021 cagttccaaa aggaaatttg ggaagtcgga gaagcgccta cctcctaccc tgatgcccta
    10081 agtttggagg aggtgttgaa ccttaccgcc gatgacgatc tgtttaagaa gttcagttat
    10141 acatacggct accttctggt gtccgacttg ctcaaactgg ctcttgatgc gaacgccaaa
    10201 ttgtatgccc aagggggatt ggacttcgcc ggctccaatg atccgctgat cgatgagttt
    10261 ggaatgcaaa tgaccttcta tgagtatcag atgatcttgg aggagcagta tcaggcggag
    10321 atggcggctc tcgctcagcg caaccttgaa gaagccgaaa cgttcctggc gatgaacacc
    10381 aagaaagagg gtgtcgtaac gacggagagc ggcttgcagt atacgatcct tgaagaagga
    10441 tcgggaccga agcccacggc ggatgatcag gtggaagtgc attacacgct ctatctgctc
    10501 aatggccagc ttgttgaatc ctcgaaagat tccggacaaa gcgtccactt ctcagcgggc
    10561 aacctgatcg agggattcac cgaagcgttg cttacgatga acgttggctc gacaattcga
    10621 acatggatcc atccctctct gggatacggc gaatatggca gccaggctat tgaaccgaat
    10681 gcgctgttga tctttgagat cgagttgctt ggaattgatg aataaccgaa gcagtgcctg
    10741 cttgaggacc gaaggcgata tcttcggtcc tttcttgtca cggtaaaaag aagaccactc
    10801 gtcgaacgat cttctcctgc gaataggtct gtttactctt gtagcgctca ccttttctga
    10861 ggtagatgaa gagtccctcc gtaaagacgg tggaaaggag cacctctttg ccttcagaca
    10921 aagagaaaag ctcctcccct tcgagaagaa caagcaggcg gttttcctct gcaacggcta
    10981 cgcttccctc ttcagtcgtc cggtaggcag cgacctgata gcgaaccgaa tcgacgaaat
    11041 gctcaccatc gggatcatca tacggcaggg agcgatcgag gatatcgatc acgcgctgcc
    11101 acgacggatg cgtgcctcga taccattcaa ggcgatccag gagatcgatc agcatcagcg
    11161 gactttcgac ttctgccact cttcatcaaa gtggatgatg tgaagcgcac tcccctcaat
    11221 tcccagcttg gctgaattga tcgcaagatc gtaggttcgg cagcggcccc atttccggtc
    11281 gctgtagtag ttgtagaagt tcgcccgttg cttgtcggtt ttgatgcaga tatcttccgc
    11341 tttgttcgcc ggagcttcat acatcgtgat cgctcgttcg actcggtagg ccagatcggc
    11401 atggatgaag atgttcaagg tgttttccat atctcgaagg atatagtccg cacatcgtcc
    11461 cacgatgacg cacggcccct cgccggcaag gcgcttgatc gtttttgact ggattaggaa
    11521 gagctggtca ttcagcggca tttcggtaac gccaagagct ccgctggcca tcggatagtt
    11581 gcccattacc aaagaataga agatgctgct tgtcgctttt tcatcggcgt tcgtgaagag
    11641 cgattcgctc aagccgcttt cccgggcgct gatagcgatc agttctttat cataaaaagg
    11701 aatatcgagc tcttttgcaa ctcgccttcc gatctcggtt ccaccacttc catactgtcg
    11761 accgatagta aacacggtat tggtactcat acgctaagct ccttatctac ctgcagtata
    11821 ccatatcaat acaaaaagaa gatacaacca cctaccaatg cgagccaatc tttcttcgct
    11881 ccgcataaaa aagatgctgc tgggcaattc ctccgtacgg ggaaaagaat gagggatcct
    11941 ttccgggata atggtgcgca agaattcttc cggtccacgt gtcgatggga aagacatcct
    12001 tccgtccata ggcgaaaaga agaatgcagg aagcgacttt cggtccgact ccatggattt
    12061 gacgcaatgt ctgcagggct tcttcatctg ccaaatccga aaccgttgcc aaaaggttgc
    12121 tctttctagc tgcatcaagg aggtattcag cccgatatcc gaagccgaga gcccgcaact
    12181 tctcttcgga actcccacga agaagttcat caggggttgg aagcgcatga tgattcgggg
    12241 caacctcctt tccataggtt tgtcttaaag tattcaacag ggttcggatc cgtttgatcg
    12301 tattgttctg actggtgatg aagctcagcg tcgtctccca gggatcctga ttgagaatgt
    12361 ggattgatct atgggcaagc agcaactctc cgagcatcgg atcgaggatc tccaagtgag
    12421 cttccgcttc ctcgtaggga tactcaagat cgaaatagcg acgcaggaat ggatccgatc
    12481 caatcgttga aaagggatcg cttgcatcaa gcgagacaag gtgccgacca atgacacccc
    12541 gataggtcga tccgtctttt tgccaggcga acgtctgccc gctgaagagc gtcgcttcca
    12601 gattgaacat gagcaatcgt agcaaaagga aaaagatggt gcaaccttca aatcctcttc
    12661 caaaatccgt aaaagctcgg tatcttcgat tatggaaagg gaatcatctt gagtgccggt
    12721 ctttgttcga ctgtcagcac tcttgatcta gattcccttt tccatatccg gcaacggctc
    12781 gtcatggaac cattgccctt gttcgaaaaa ccaacatcag cgcagtggta tatttcccta
    12841 cattcatcag ccatatcatt gtattttcat gtttcactgt ttacagtggc acaccaccgt
    12901 gatgaaaggt gtgtatttga tgaaaactgc ttgtcaacag gtctgcaaaa cgctttgact
    12961 gacagcctca acgattgcca ccagacatct cgaccgcaac cgcaagcagg gatctgaagc
    13021 aattgctgca agatggtaaa ctgaaatcct ccggttccgg acgaatgacg aactatcgaa
    13081 aaaagagaca tgaaggctga acggaaacat ctgttgggta ttccttgatc ttatgatgaa
    13141 tctcctactc ctgttcaaat aaccaggtgg agagataccg ttcaccggta tcgggaaaga
    13201 caacgacgat cgtcttgtcc c
//
LOCUS       DAOSLI010000045        13122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_4805_length_13122_cov_25.285758, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000045 DAOSLI010000000
VERSION     DAOSLI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4805_length_13122_cov_25.285758"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1438
                     /locus_tag="PLF67_07015"
     CDS             35..1438
                     /locus_tag="PLF67_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89793.1"
                     /translation="MNIITRSIEPMHFDLLHQTITDPYSPETYKNRINRAVAKAEEHG
                     FSHLVIYADREHFANLTYFTGFDPRFEEALLILAKGMEPILLVGNEGYSYCTIIPYQL
                     DVVLYQSFSLMGQERVPTTKNVLTESYEKAGITGASRIGLIGWKYFRDVEGSDPDYMT
                     DVPHYLVSSLQAIVGKERVLNATSLLINANDGLRTTVDVDELAVLEIAATKASRGVQN
                     MFLNLEEGMSEIEASYYLQFDGNPLVAHPNVNFTPKGVLQGTASPSRHPLTYGSSMNV
                     GFGYRGSMCARTGVYVYDRTDFDKYYPTSWEAAYVPYFKMTALWYELVRIGNTGKAVV
                     EELLRRVPEFSKLGIGLNPGHLIHSDEWTNSLFVTHEAIELRSGMAIQCDVIANPPGH
                     PGLHIEDGLVIADADLRAAFKTKYPNAWKRIERRRKVMKEILGIEIGDQILPLSDIQG
                     VYYPFGADLSTVMAVER"
     gene            complement(1511..>1767)
                     /locus_tag="PLF67_07020"
     CDS             complement(1511..>1767)
                     /locus_tag="PLF67_07020"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOE89794.1"
                     /translation="EEIDIVARTADGELIFGECKFTNAQVCSRELHELMRKSENIRKG
                     NQSGRYYLFSKSGFTKELIQRSKEREEVVLVSLNDLMKRI"
     assembly_gap    1768..1867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1868..2959)
                     /locus_tag="PLF67_07025"
     CDS             complement(<1868..2959)
                     /locus_tag="PLF67_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE89795.1"
                     /translation="MRFVGRESELKEFHEIFTSSRFEFVVVYGRRRIGKTTLISKACE
                     GHRTIFHLAAKTTGTKTLSDLSTAAAAGLDEGFGIGFPTLEVFLEYIAEKAKEEPLIL
                     VIDEFPFFCASIPEAMGVFQRCIDLRFPKTRLKLVLSGSSVSFMEHQVLGDKSPLYGR
                     RTAQFKILPFSLQETKELYGGSDIDTVRVQAITGGVPLYVSYFSPKKNLEEEIARLFF
                     HVNGLLYNEPMNILNMEVNQPQVYFTVLELMARGVNKHIEISDKSGLSPTQASYYLST
                     LRELGLVADILPFGEKNKKRTLWVIKDGLFLFYFKHVYPYRALIERGRNDGVTAQLKK
                     SFSAHMGKPFEEICKEYLLIHPELMIIEIG"
     assembly_gap    3047..3146
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3488..4855
                     /locus_tag="PLF67_07030"
     CDS             3488..4855
                     /locus_tag="PLF67_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOE89796.1"
                     /translation="MKVLIFGLGLHGGGYAAASYFLDHGHEVRITDLRAEEDLKNQVA
                     SLVNRGATYSLGNHRKDDFLWADVVVKNPAIPPSHPLLKDARVVINDFSWLFSSPWIQ
                     EVKIIAITGTKGKTTTSAAVAHVLAHLGVEAMQCGNMGISGFTVLKEFEDRRKQGRDL
                     PVYLVCEFSSWQIRDTIVALAGAMPHLEICALTSFYPDHLNTYESIERYMEDKLLLFG
                     PFVKKAVIPQIIARRVRQQTGLDRKRIRPSDTTVPKELIEHPNLHSAYAILLSLGFKY
                     REVLEALGSFTGVPHRIEAIGSLRNILFINDSAATIPEAVHFTYGNFKLLAVHLITGG
                     TDKRLDPNSMVEELRQALSVSLLGGSFTTDKLIPLLERENIPYFGPFDSMGAALEASL
                     DSALKKERELPDLQQVILLSPGSASFELFDNEFDRGDQFRTLVNLLILAEETARTDTD
                     GAPEP"
     gene            complement(4800..5735)
                     /locus_tag="PLF67_07035"
     CDS             complement(4800..5735)
                     /locus_tag="PLF67_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOE89797.1"
                     /translation="MKIKPIRAEELTGEDSPLQSLFWARAKSILGWKPYAFEINEEQR
                     LLLLVRRIAPLFFFAYVPFGPSGPVDLSMLSEGLKQAIDEPVFAIRYDLPFTDTAFET
                     GGLLSLSYSIQPERTVRIDLQKPLVYRKRALRALKKCENRYTVRPYRGEPILFEQWYE
                     TYRQTALRDRFHPRSKEYFFHLLSIDEDKMVKPILLLAFGQNEVVGGVLLIEGKKETI
                     YLAGSSTRPNVGYLLQDGAIRRTKERGGELYDLFGISNQSGYLASLDLFKTAFGGEIV
                     KREPSLDYPTRALRYRLFTVLERHRYRFSRSLQQG"
     gene            complement(5742..7946)
                     /gene="metG"
                     /locus_tag="PLF67_07040"
     CDS             complement(5742..7946)
                     /gene="metG"
                     /locus_tag="PLF67_07040"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOE89798.1"
                     /translation="MTVMKKRLITSALPYVNNIPHLGNLIQVLSADVFARFCRSKGYE
                     TLYVCGTDEYGTATETRALQEGVTPRELCDHYFVIHRDIYRWFDIRFDYFGRTSTPAQ
                     TEIVQGIFRKVDERGYIMEHEIEQLYCPTCDRFLADRFVEGTCPVCSYEKARGDQCDH
                     CQSLLEPTELINPHCGVCGTKPELRKTRHLYLDLPKALPLLEAWMDRASKEGFWANNA
                     IQVTRSWIRDGLKERAITRDLKWGIPVPKEGYENKVFYVWFDAPIGYISITSEATSEW
                     KSWWQDPKNVELFQFIGKDNIPFHTVIFPSTLLATGDDWTMLHHMSSSEYLNYEGGKF
                     SKSLGVGIFGNDVQDTKIPVDVWRFYIFYNRPETSDFTFTWNDFQEKVNGQLIGNLSN
                     LVNRTLTFVNRFYKDDPDLFDAEIDTNLYDEVKAIETKIDEILERSELREGLRQILAL
                     SSVGNKAFQDGEPWKARTENPEKAKSLLKTLLYVIRDLGILVEPYMPQTSREILSFVG
                     SEDAKWSDLGSTDAVLTLREPTLLFEKLEDDRIAELRERFSGSQDQRKQEMKEEPMEE
                     LAQGRIFAEKVILKVAQITEVEKHPNGDRLYILTLDANEDEERTIVSSIVPFYTEEEL
                     LGRKIVLVSNMKPSNFRGVKSRGMLLAANDPEAEEHTTCEVIFVDDFETGAILEVEGF
                     DPPQANIPYVKPDLFFSLELYAEDGVVKVDGRPIGKAGRHLRTVTYLNGPVN"
     gene            8042..9016
                     /gene="hflK"
                     /locus_tag="PLF67_07045"
     CDS             8042..9016
                     /gene="hflK"
                     /locus_tag="PLF67_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOE89799.1"
                     /translation="MDNRNQRPVRPIRFNISPKLVIWIVIAVVAILMVLGSFFVVDQT
                     EQAVVLRFGRYHRTVGPGLQTKIPLGIERNYNVPTQVVQTMTFGYRGQGAASPLYGQN
                     YTNESLMLTGDLNIIDVQWTVQYKIENPYNWMFKVESRESTLRDISQSIMNQLVGDLP
                     ILSVMTSQRTRIEVEAQEMMQKMFDLYGLGIRVVTVKLQNIVPPVGSVQDAFEDVNKA
                     IQDMNRYINEGKKLYNEVIPAARGEANRLIQVANGYAAERVNQARGDVERFNSVREVY
                     ENSKDITRTRLYIETLEEILQDNNGKVTLVDKNLTNFLPMQMLQGGQQ"
     gene            9013..10002
                     /gene="hflC"
                     /locus_tag="PLF67_07050"
     CDS             9013..10002
                     /gene="hflC"
                     /locus_tag="PLF67_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOE89800.1"
                     /translation="MMNRSQKRVLTTVVVVIVLLVIFIILGPFYILVEGQQAVVTRFG
                     KIIDVQDQSGLKLKMPLIDNVVYYPKKVLSWDGAAQRIPTKENQFIWVDTTARWLISD
                     PARYYETVNTIDNGIARLNDILDSSVRTIISENYLNEAVRTTNQINEITVEEDVQSLD
                     SAEDAEQLRNLTATQTKQENIRIGREQLSAKMNIQAEQFTDGFGIKLIDIIIRQIRYS
                     DDLTESVYQRMIKERNQIAEAYRSYGRGQLAQWQGKTESEQRVILSTAYAESEAIKGV
                     ADAEAAAIYSDAYEADPEFFNLWRTLESYRKTIPDLDKVLSTDMEYFDQLYGK"
     gene            10074..10739
                     /locus_tag="PLF67_07055"
     CDS             10074..10739
                     /locus_tag="PLF67_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE89801.1"
                     /translation="MAKPNLVIFDFDGTLYPFRPYDSEQTLVLSHAKTKGPLFRLRAR
                     LFVRADRRGAFNNPQFYKRYEKMIRDVDPELVEQTARDLAGLLSEEDRAAIRALTEVA
                     DLAILSLGTENLAEFFLEELGLREEFFLIKAKRIRWKKGKAYLKVNIGEAEGKRTNLE
                     ALRKTYKKIVAIGDGPTDIPMLEAADLGLLLIWGAESSPHPFELHSSLPSACNRAKEF
                     LTS"
     gene            complement(10736..12376)
                     /locus_tag="PLF67_07060"
     CDS             complement(10736..12376)
                     /locus_tag="PLF67_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89802.1"
                     /translation="MRIPVVLLLLKSYLKGTKPLRSVTQRFSFGKKKSVRTISGVVLS
                     LLMLASLMSFVFLLGTNYYNYQLVGMMVGVPHVGLLMGAAFATLSLLIFAFPAAINIL
                     HAAKEIERLRALAISESELALSRMIIFYFNFFPIYLFFVIPALIVGIMTTGFSFFYLL
                     SSLLLLLVGPMIPISLVSLLEVGVVHLTKGRRAQRSGELFYLVVMMALVIGISSQMGK
                     NAEMTGNLEGLTHQLAPVIRKLTRFLAPFALQAQGLYNPILLLVWLAGALLLAYITFT
                     VTAGTYHHACSLLASGGYRTKKRRKNNTGEQKPIVALMKRDWTILLSTSGFIFELGGE
                     LLIPLILIITYTAMGIIGDISQATKVLAQYPIFEPVVVLIVLLMSNINLLSCTSVSRQ
                     GKLALHDRLYPLEPKVFVNAKFYLHMALVGTTNTIYLLAATLILKLSIGNLWFYVMLS
                     LISIAGTSYFHLAIDYHNPLLEWTSAQQAMKRNPNGFLGMLVSFLFVVWVALFLVGLP
                     YFFEISQSNALMAALGMAVVTAKAGQRLAYRSAKGFLA"
     gene            complement(12376..13098)
                     /locus_tag="PLF67_07065"
     CDS             complement(12376..13098)
                     /locus_tag="PLF67_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89803.1"
                     /translation="MLICDKLYKRYAKNAPYAVEDLTLKVGEGEIFGFLGPNGAGKST
                     TIKMIVSLLNSDSGEVSLDGITAKDDPIAYKRLIGYVADEPQFYDKMSANEHLAFIGD
                     LYRIPQDERTERISKLAVTLRLQDALDDEIGSFSHGMRQKLAIISALLPAPKLLVLDE
                     PMVGLDPKASFEVKNLLKELAKEGKIVFFSTHVLEVAQQLCTSLGIINEGKLIYHGTF
                     EALQNERDESLEELFLELTEKG"
BASE COUNT         3125 a         3483 c         3403 g         2911 t
ORIGIN      
        1 tggacactac gaactgtatt tctaaggagc aagcatgaac attatcacca gatcgattga
       61 accgatgcac tttgatctgt tgcatcaaac aattaccgac ccgtacagcc cggaaacgta
      121 caaaaatcgg atcaatcgag cagtagcaaa agcggaagag catggttttt cccatctcgt
      181 catctatgcc gatcgcgagc actttgcaaa cctcacctat ttcaccggct tcgatccgcg
      241 ctttgaggaa gcgctcctca ttcttgcaaa aggaatggaa cctattctgt tggtgggcaa
      301 cgaggggtac agctattgca cgatcatccc ctatcagttg gacgtcgtgc tgtatcaatc
      361 gttctccctc atgggtcagg agcgggttcc gaccacgaag aacgttctga ccgagtccta
      421 tgagaaagcg ggcatcaccg gtgcaagccg aatcggcctc atcggttgga agtatttcag
      481 ggacgtcgaa ggctcggacc ccgattacat gaccgatgtt ccgcactatc ttgtttcatc
      541 cctccaagcg atcgtcggca aggaacgggt cctcaatgcg acgagccttc tgatcaatgc
      601 caatgacgga cttcgcacca ccgtggatgt ggatgagctg gccgttcttg aaatcgcagc
      661 aaccaaagcc tcgaggggag tccagaatat gttcttgaac ctcgaggagg gaatgagtga
      721 aattgaagca tcttactatc tccaatttga cggaaacccc ctggtcgccc accccaacgt
      781 gaacttcact ccgaagggag tgcttcaggg aaccgccagt ccctcccgcc acccgctgac
      841 ctacggttca tcgatgaacg tgggcttcgg ctaccgcggt tcgatgtgcg cccgaaccgg
      901 agtgtatgtg tacgaccgga cggatttcga caaatactat ccaaccagct gggaggctgc
      961 gtatgtgccg tacttcaaga tgaccgcgct gtggtatgag ctggtccgaa tcggcaatac
     1021 cggaaaagcg gtggtcgaag agcttttacg acgggtgccc gagttctcca agctggggat
     1081 cggcctgaat ccgggacacc tgattcacag cgatgaatgg acgaactccc tcttcgtgac
     1141 ccatgaagcg atcgagctgc gaagcggcat ggcgatccag tgcgacgtca tcgccaaccc
     1201 gccgggacac cccgggctcc atatcgaaga cggcctggtg atcgccgatg ccgaccttcg
     1261 agccgcgttc aagaccaagt atcccaacgc ctggaaacgg atcgagcgcc gaaggaaggt
     1321 gatgaaggag attctcggaa tcgagatcgg agaccagatc ctgccccttt ccgacatcca
     1381 aggcgtctac tatccgttcg gagcggacct gagcaccgtc atggcggtcg agcgatagca
     1441 gcaaaaagat aaaacacaca ggtgaagcgc ctctttgtag agacacttca cctgtgtgcc
     1501 cattacatta tcagattcgc ttcatcaaat cattcaatga tacgagaacg acctcttctc
     1561 tctccttgct tcgctggatg agctccttgg taaaaccgga tttggaaaaa aggtagtatc
     1621 gaccactctg attgcccttc cgaatgttct cgcttttacg catcaactca tgtaattcgc
     1681 gggagcaaac ttgggcattt gtgaacttgc attctccgaa gatgagttcc ccatcagccg
     1741 ttcttgctac gatatcgatc tcttcttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnccc tatttcaatg atcatcaatt caggatgtat caggagatat tccttgcaga
     1921 tctcctcaaa cggtttaccc atatgggcgg aaaagctttt tttcagctgc gccgttacac
     1981 cgtcattcct tcctcgctcg attaacgccc gatagggata cacatgtttg aaataaaaca
     2041 agaaaagtcc atctttgata acccatagcg ttcgtttttt gttcttttct ccaaacggca
     2101 ggatatccgc tacaagaccc aactcacgga gggtggaaag ataatagctt gcctgagtgg
     2161 gtgaaaggcc gcttttatcg gaaatttcaa tgtgtttgtt caccccgcga gccatcagct
     2221 ccagaacggt gaagtacacc tgcggttggt ttacttccat attcaggata ttcatcggtt
     2281 cgttgtacag aaggccgttg acatgaaaaa agagacgggc gatctcttct tccaagtttt
     2341 tcttcggtga aaaatatgaa acgtagagcg gcactccacc ggttatcgcc tgcacacgga
     2401 cagtatcgat gtcactgccg ccatagagct ctttggtttc ctgtagggaa aacggaagga
     2461 tcttgaactg ggcggttctt cgaccataca ggggactttt gtcaccgagc acctgatgct
     2521 ccatgaaaga caccgaagaa ccggaaagca ccagcttgag tcgggtcttg ggaaagcgta
     2581 aatcgataca tcgctgaaac acccccatcg cttcggggat tgatgcacaa aaaaacggaa
     2641 attcatcgat gacgaggatg agcggctctt ctttggcctt ctcggctata tactccagaa
     2701 agacttccaa tgtaggaaag ccaataccga atccttcatc gagccccgct gcggcagccg
     2761 ttgaaagatc ggaaagggtt ttcgtcccgg ttgtttttgc cgccagatga aagatcgttc
     2821 tgtgcccttc acatgctttg gagatgaggg tcgtttttcc tatccgacgg cgaccataga
     2881 ccactacaaa ctcaaaacgg gacgaggtga agatctcatg gaactctttc agctcggatt
     2941 ctcttccgac aaatctcatc gcttatcctt ccctccgttc ggtgtatccc cagagtagca
     3001 gaagaaatgg aagcaatcaa gtcattcaaa tacgttttaa taaaatnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnaata aaactctcct atcaaggttg aaactcagca
     3181 gtttcttgtg tatgggtacc gcgcaaaatg ggtgtctgaa aagtggaacc ttttaacccg
     3241 ttgctgacaa attgatattc aatgatagaa atggcatgga tatccgtaca ctcctacagc
     3301 ctgatcgaac atcaatcaca gagcttatac gggaactgtc agcctcctgc cggaacaggg
     3361 cgcatggttt cccacatggc aaaccatgta cgataaagga cggagcaaat atctgtttta
     3421 gcgctccatt cgatgatttt acttgcactc tccccctctt cttcctacac tgtccccagg
     3481 gggtaggatg aaggtattga tcttcggttt ggggcttcat ggcggtggat atgctgcggc
     3541 atcctatttt cttgatcatg ggcacgaagt gcgaatcacg gatctgcggg cggaagagga
     3601 tttaaagaac caagtagcat ccctcgtgaa tcgaggggcg acgtacagtt tgggcaatca
     3661 tcgaaaagac gattttctct gggccgacgt ggtggtgaag aatccggcga ttcctccctc
     3721 ccatcctctt ctgaaagacg cccgggtcgt gatcaacgat ttttcctggc tgttttcatc
     3781 cccatggatc caggaagtga aaatcatcgc catcacgggt accaagggga aaaccacgac
     3841 cagcgccgcc gtggctcacg ttctcgccca cttgggggtg gaggcgatgc agtgcggaaa
     3901 catgggaatc agcgggttta ccgtcctcaa agagtttgaa gaccgaagga agcagggaag
     3961 agacctaccc gtctatctgg tctgcgaatt ctcttcctgg cagatcagag acacgatcgt
     4021 cgctcttgcc ggggcgatgc ctcacttgga gatctgcgct ctgacctcct tctaccccga
     4081 tcatctcaac acgtatgaat cgatcgaacg ctatatggaa gacaaactgc tcctcttcgg
     4141 tcccttcgtg aagaaagcgg tcattcctca gatcatcgct cgacgggtaa gacagcaaac
     4201 cggtctggac cgcaagcgca tccgaccgag cgacaccacc gtacccaaag agctcattga
     4261 acatcccaac ctccacagtg cgtatgcgat tctcctctcg ctgggcttca agtaccggga
     4321 agtcctcgaa gcgctcgggt cgttcaccgg ggttcctcac cggatcgaag cgatcggaag
     4381 cctcaggaac atcctcttca tcaacgacag cgccgcaacg atccccgaag cggttcactt
     4441 cacctatgga aacttcaaac ttctggcggt tcatctgatc accgggggaa ccgacaagcg
     4501 cctcgatccc aattccatgg ttgaagagct tcgtcaagcc ttgagcgtca gtctcctggg
     4561 gggaagcttc accaccgaca agttgattcc tttgctcgaa agagagaaca ttccatactt
     4621 cggccccttc gattcaatgg gagcggcgct tgaggcgagc ttggacagcg ccctgaaaaa
     4681 ggaacgggag cttcccgatt tgcagcaggt gattctgctc tcgccgggaa gcgcttcctt
     4741 tgagctgttc gacaatgagt ttgaccgcgg cgaccagttc aggacactgg tgaatctcct
     4801 catccttgct gaagagaccg cgagaaccga taccgatggc gctccagaac cgtgaaaagc
     4861 cggtaccgta gggctctggt cgggtagtca agagacggct cgcgtttgac gatctctccg
     4921 ccgaatgcgg tcttgaacag atccaaggaa gcgagatacc cgctttggtt ggagattccg
     4981 aagagatcgt agagttcacc gccccgttcc tttgttcgcc gaatcgctcc atcctgcagc
     5041 agatacccta cattgggcct ggtcgatgat cctgcgaggt agatcgtttc tttcttccct
     5101 tcgatgagca gcactccccc gaccacttcg ttctgcccga aagccagaag gagaatcggt
     5161 ttcaccatct tgtcttcatc gatcgaaagg agatggaaaa agtattcttt gctccgcggg
     5221 tggaaacggt cgcgaagagc ggtctgccta taggtttcat accactgctc aaagagaatc
     5281 ggttcacccc ggtaaggtcg gaccgtgtac ctgttctcgc acttcttaag cgccctcaga
     5341 gcgcgtttgc gatagacaag cggcttttga agatcaatcc gaacggtccg ctccggctgg
     5401 atcgaatagg ataatgataa aagacccccc gtttcaaatg cggtgtccgt gaacggaagg
     5461 tcgtagcgga tcgcgaaaac cggttcatcg attgcctgct tcagaccttc ggataacatc
     5521 gataagtcga ccggcccact cgggccgaag ggaacatatg cgaaaaagaa gagcggagcg
     5581 attctgcgaa caaggagcag cagccgctgc tcctcattga tttcaaaagc gtagggtttc
     5641 cacccgagga tcgactttgc ccgagcccaa aagagcgatt gaagcggaga atcttctccg
     5701 gtgagttctt ctgctctgat cggcttgatc ttcatacacc tttagtttac cggaccgttg
     5761 aggtatgtga cggttctcag gtgcctgccc gcttttccga tcggtcgtcc gtcaaccttt
     5821 acgaccccgt cttccgcata cagctcaagg gaaaagaaga ggtccggctt cacataggga
     5881 atattcgctt gtggcggatc gaatccttca acttccagga tcgctcccgt ctcaaaatca
     5941 tcgacaaaga tcacttcgca cgtggtgtgc tcttccgctt cgggatcgtt cgccgccaac
     6001 agcattcccc tgctcttcac tccccggaag ttcgagggct tcatgttgct gaccagaacg
     6061 atcttccggc ccaggagctc ctcctcggta tagaagggaa cgatcgagct gacgatcgtg
     6121 cgttcctcat cctcgttcgc atccagggtg aggatataga ggcggtcgcc gttggggtgt
     6181 ttttcaacct cggtgatttg ggcgactttg agaatcactt tttcggcaaa gattcgacct
     6241 tgtgcgagtt cttccatcgg ttcttccttc atttcctgct ttcgctgatc ttgagagccg
     6301 ctgaatcgct ctcgaagctc ggcaatgcgg tcatcctcca gtttctcaaa gaggagggtc
     6361 ggttcccgca gggtcaatac ggcatcggtc gatcccagat cgctccattt cgcatcctcc
     6421 gaaccgacaa aggagagaat ttcccgactg gtctgaggca tgtacggctc gacgaggatt
     6481 cccagatcgc gaatcacata cagcagcgtc ttgaggaggc tcttcgcctt ctcggggttc
     6541 tcggtgcggg ccttccacgg ctcgccatcc tgaaacgcct tgttccccac cgaggagagg
     6601 gcgaggatct gcctgaggcc ttcgcgcagt tcgctccgct ccaagatctc atcgattttc
     6661 gtttcgatcg ccttgacctc atcgtacaga ttcgtatcaa tctccgcatc gaacagatcg
     6721 ggatcatcct tgtagaagcg gttgacgaag gtaagggtcc ggttcaccag attggagagg
     6781 tttccgatca gctggccgtt caccttctcc tggaagtcgt tccacgtaaa ggtgaagtcg
     6841 cttgtttcag ggcgattgta gaagatgtag aagcgccata cgtcgacggg gatcttcgta
     6901 tcctggacat cgttgccgaa gatccctact ccgagacttt tggagaactt gcccccctcg
     6961 tagttgagat actcgctgct ggacatgtga tgaagcatcg tccaatcatc tccggtcgcc
     7021 agcagcgtcg aggggaagat gaccgtgtgg aagggaatgt tgtcctttcc gatgaactgg
     7081 aagagttcga cgtttttcgg gtcctgccac caggacttcc actcgctcgt cgcctcgctg
     7141 gtgatcgaga tgtatccgat cggggcgtcg aaccacacat agaagacctt gttctcatac
     7201 ccctctttgg gaacgggaat cccccacttc agatcccggg tgatcgcccg ttccttcagg
     7261 ccgtcgcgga tccatgagcg ggtgacctgg atcgcattat tcgcccaaaa cccctctttg
     7321 ctcgccctgt ccatccatgc ttccaaaagg gggagcgctt tgggcaggtc aagatagagg
     7381 tgcctggtct ttcgtaattc cggtttggtg ccgcacaccc cgcagtgagg attgatgagc
     7441 tcggtcggtt ccaaaagact ctggcaatga tcacactgat ctccccgggc tttttcatag
     7501 ctgcagaccg ggcaggttcc ctcgacgaag cgatcggcca ggaagcgatc gcaggtggga
     7561 cagtacagct gttcgatctc atgttccatg atataaccgc gctcatcgac cttcctgaaa
     7621 attccctgga cgatctccgt ctgagcggga gtcgaggttc gaccgaaata gtcgaaccgg
     7681 atgtcgaacc accggtagat gtcgcgatgg atgacaaaat aatggtcgca cagctcgcgg
     7741 ggcgtcaccc cttcctgcaa agcccgggtt tccgtggcgg ttccgtattc atccgttccg
     7801 catacataga gggtttcata ccccttcgat cggcaaaacc gggcaaacac atccgcgctg
     7861 agaacttgaa tgagattccc caggtgaggg atgttgttca catagggcag agcactggta
     7921 atgagtcgtt ttttcatgac cgtcatcata gaccgccggt attttctttt caatggttca
     7981 ccttgactat gctttgatgc gtaagtacat ttagggctgt gctaggcaca aaggagacag
     8041 catggataac aggaaccagc gacctgtccg cccgatccga ttcaacatca gccccaagct
     8101 ggtcatctgg atcgtgatcg cagtggtggc gatcctcatg gtgttaggca gtttcttcgt
     8161 cgtcgaccag acggagcagg ccgtggtatt gcgctttggc cgctaccatc gaaccgtcgg
     8221 gccgggcctt cagacaaaaa tcccgcttgg aatcgaaagg aattacaatg tacccaccca
     8281 ggttgttcag acgatgacgt tcggctaccg gggtcagggt gccgcttcac cgttgtatgg
     8341 acaaaattac acgaatgaat cgttgatgct caccggagat ttgaacatca ttgacgtcca
     8401 gtggaccgtc caatacaaga ttgagaaccc ctacaactgg atgttcaagg tggagtcccg
     8461 ggaatcgacg ctccgggata tcagccaatc gatcatgaat cagctcgtcg gcgaccttcc
     8521 gattctctcg gtgatgacca gccaacgaac ccggatcgaa gttgaagctc aggagatgat
     8581 gcagaagatg ttcgaccttt acggtctggg aatccgcgtc gttacggtaa agcttcagaa
     8641 catcgttcct ccggtgggaa gcgttcagga tgcgttcgaa gatgtcaaca aggcgatcca
     8701 ggatatgaac cggtatatca acgagggcaa gaagctctac aatgaagtca tcccggccgc
     8761 ccggggagaa gccaaccgcc tcatccaggt agccaacggg tatgccgccg aacgggtgaa
     8821 ccaggctcgg ggagacgtcg aacgcttcaa cagcgtccgg gaagtctatg aaaactcgaa
     8881 ggatatcacc cgaacccggc tctacatcga aacgcttgag gagattcttc aggacaacaa
     8941 cggaaaggtt acgctggttg acaagaacct gacgaacttc cttccgatgc agatgcttca
     9001 aggaggtcag caatgatgaa tcgctcacaa aaaagagttc tgactaccgt tgtcgtcgtt
     9061 atcgttctcc ttgtaatctt catcatcctc ggaccgttct acatccttgt cgaaggccaa
     9121 caggcggtag tcacccgctt cggcaagatc atcgacgtgc aggatcagag cggcctgaag
     9181 ctgaagatgc ccctgatcga caatgtcgtg tactacccga agaaggttct ctcctgggac
     9241 ggagccgccc agcggattcc gacgaaggaa aaccagttca tctgggtcga caccaccgcc
     9301 agatggctga tcagcgatcc ggccagatat tacgagacgg tgaacacgat cgacaacggc
     9361 atcgcccggt tgaacgatat tctcgattcc tccgttcgga cgatcatcag cgagaactac
     9421 ctcaacgaag cggtccggac caccaaccag atcaacgaga tcaccgttga ggaggatgtc
     9481 caaagcctcg acagcgctga agatgccgaa cagctgcgga atttgacggc aacccagacc
     9541 aaacaggaaa acatccgaat cggacgggag cagttaagcg ccaaaatgaa cattcaagcg
     9601 gagcagttca ccgacgggtt cggtattaaa ctgatcgata tcatcattcg tcagatccgc
     9661 tacagtgacg acctcaccga aagcgtctat cagcggatga tcaaggaacg aaatcagatc
     9721 gccgaagcat atcgatcata cgggcgcggt cagcttgccc agtggcaggg aaagaccgag
     9781 agtgaacagc gggtgatcct ttccacggca tatgcggaaa gcgaggcgat caagggtgtc
     9841 gccgacgccg aggccgctgc gatctacagc gacgcatatg aagcggatcc ggagttcttc
     9901 aatctttggc gaacgctgga atcgtaccga aagacgattc ccgacctgga caaggtgctt
     9961 tccaccgata tggagtactt cgatcaactc tacggaaagt aaggaattac catttggtgt
    10021 caggggaagg cgaccgccct tccccttttt ttcttctcct gctatggttg gccatggcca
    10081 aaccgaatct cgtgatcttc gattttgacg gaacgctcta tccgtttcgc ccctatgaca
    10141 gcgagcagac gctggttctc tcccacgcaa agaccaaagg tcctctcttc cgtctccgcg
    10201 cccgtctctt tgtccgcgcc gaccggaggg gagcgttcaa caacccgcag ttctacaaac
    10261 gctacgagaa gatgatccgc gatgtcgatc ccgagttggt tgaacagacc gccagagatc
    10321 tggcaggact cctcagtgaa gaggacagag cggcgatccg ggcgctgacc gaggtcgccg
    10381 atctggcgat cttaagcctc ggaacggaga acctcgccga attctttttg gaggagcttg
    10441 gcctacgaga ggagttcttc ctcatcaaag caaaacgaat ccgctggaag aagggcaagg
    10501 cgtacctgaa ggtcaatatc ggcgaagcgg aaggcaaacg caccaacctc gaagctttaa
    10561 gaaaaaccta taagaaaatc gtcgcgatcg gtgacggtcc gaccgatatt ccgatgctcg
    10621 aggcggcgga tctgggtctt ctgctgatat ggggagcgga atcctctccc cacccctttg
    10681 agcttcactc atcgctgccg tcggcgtgca accgggcgaa ggagttcctc acctcttagg
    10741 cgagaaagcc ctttgcactt ctgtaggcaa gcctttgacc ggcctttgcg gtgaccacgg
    10801 ccatgccgag ggcggccatg agagcattgg attgggagat ctcaaagaaa tacggcagac
    10861 cgaccaggaa cagggcgacc catacgacga acaggaagga aaccagcatc ccgagaaagc
    10921 cgttggggtt gcgcttcatc gcctgttggg cgctcgtcca ctccaacaga ggattgtgat
    10981 aatcgatcgc cagatggaag tagctcgttc cggcgatcga gatcagggaa agcatgacat
    11041 agaaccaaag attcccaatg gagagcttga ggatcaaggt agcggcaaga agatagatcg
    11101 tattggtcgt accgacaaga gccatgtgaa gataaaactt cgcattgacg aacaccttgg
    11161 gctcaagggg atacagacgg tcgtggaggg caagcttgcc ttggcgcgat acgctggtgc
    11221 aggaaagcag gtttatattg gacatgagca gcacaatgag aacgacaacc ggttcaaaga
    11281 tcggatattg ggcgagcacc ttcgtcgcct gagagatgtc gccgatgatt cccattgccg
    11341 tgtaggtgat gatgagaatc aggggaatca gcaattcccc gcccagttca aagatgaatc
    11401 cgctggtcga gagaaggatc gtccagtccc gcttcatcaa cgcgactatc ggtttttgtt
    11461 cgcctgtatt gttttttctt cgtttctttg tccgatatcc gccggaagcc agcagggagc
    11521 aggcgtgatg ataggttccg gcggttacgg taaaggtgat gtaggcaaga aggagcgcac
    11581 ctgcgagcca cacaaggagc agaatcggat tgtacagacc ttgagcctgc aaggcaaacg
    11641 gagcaagaaa tcgggtaagc tttctgatca ccggggccag ctgatgcgtc agcccttcca
    11701 gattgccggt catctcggcg tttttcccca tctgggagga tatgccgatc accaaggcca
    11761 tcatgacgac caggtagaag agctcgcccg accgttgagc ccgcctcccc ttggtcagat
    11821 ggacaacacc gacctcgagc agggaaacca gcgaaatcgg aatcatcggt ccgacaagaa
    11881 gcaaaagcag cgagcttaag aggtagaaga aggaaaagcc cgtggtcatg attccgacga
    11941 tgagagcagg gatgacgaag aagagataga tcgggaaaaa attgaaatag aagatgatca
    12001 tccggctgag ggcaagctcc gattcgctga tcgccaatgc cctgagccgc tcgatctctt
    12061 tcgccgcgtg caagatgttg atcgctgcgg gaaacgcgaa gatgagcaga ctcaacgttg
    12121 cgaatgccgc gcccatcaaa agaccgacat ggggaacgcc gaccatcatt ccgaccagtt
    12181 gatagttgta gtagttcgtt ccaagcaaaa atacaaagct catgagcgag gcgagcatca
    12241 aaagcgaaag aaccaccccg ctgatcgtcc gcaccgattt cttctttccg aagctgaagc
    12301 gctgggtcac cgatcgaagc ggcttggtcc ctttgagata ggatttgagc aaaagcagga
    12361 cgaccgggat gcgcatcagc ccttctccgt caattcaagg aagagctctt ccagactttc
    12421 atcccgttcg ttctgaagcg cctcgaacgt tccatggtag atgagcttcc cttcgttgat
    12481 gatgcccaag ctggtgcaaa gctgctgggc gacctcgaga acgtgagtgg agaaaaagac
    12541 gattttcccc tccttggcaa gctctttcaa gaggtttttc acttcaaacg acgccttcgg
    12601 atccaggcct accatcggct cgtcgagcac caacagcttc ggggcgggga gcagggcgct
    12661 gatgatcgcc agcttttgcc tcattccgtg ggagaaggag ccgatttcat catcaagggc
    12721 atcctgcaag cgaagcgtca cggccagttt ggagatacgc tcggtccgct cgtcctgagg
    12781 aatccgatag agatccccga tgaacgcaag gtgttcgttt gcactcatct tgtcgtagaa
    12841 ctgcggctcg tccgccacat acccgatcag gcgtttatag gcgatcggat catcctttgc
    12901 agtgatgccg tccagactca cctctccgct gtcactgttc aacagggaaa caatcatctt
    12961 gatcgtcgtc gatttccccg ccccgttggg accgagaaat ccgaagattt cgccttcacc
    13021 gaccttcagg gtcaaatctt ccactgcgta gggagcattc ttggcgtatc gtttatataa
    13081 tttgtcacat atgagcatga aagcagtata aaaagttcag gg
//
LOCUS       DAOSLI010000046        12735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_4984_length_12735_cov_30.595505, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000046 DAOSLI010000000
VERSION     DAOSLI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4984_length_12735_cov_30.595505"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1166)
                     /locus_tag="PLF67_07070"
     CDS             complement(72..1166)
                     /locus_tag="PLF67_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOE89804.1"
                     /translation="MAKNYSLEMAKSVINRYQPAQMQWHYEHGLVITAILEVGEHYRQ
                     TSFFDWAYSMYDPFIKEDGTIKDYRAGEYNLDMINAGRNLFLLHEKTGERRFIKAAHI
                     LREQLVGQPRTRSGIYWHKQIYPWQVWLDGVYMQGPFSALYAKYVDQREIIEDLAIQI
                     ERIYATLRDSKTGLLYHAWDESRGMRWSDLETGLSPHFWGRALGWYAMAVVDIIAIIS
                     TDDPLSDPLKKILNTLMGDILAFQQPDGMFYQVVDRSEAEGNYKETSASAMFAYTLLK
                     MARLGIGADPKIQKSALKALDGIEKRYLSIDEDGEYHLEGICSVAGLGGNPYRDGSLY
                     YYFKEPQVADDFKGVGPYILATLERETLST"
     gene            complement(1223..2800)
                     /locus_tag="PLF67_07075"
     CDS             complement(1223..2800)
                     /locus_tag="PLF67_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE89805.1"
                     /translation="MRKQMSTWLVLVTILALAAMPLFSQGAADKKASEGTTVIELWYG
                     AAMTEAGPPPEDWVGFQIIKDKLNIDLKITALPSSEGDQDVKIQAAAAANNLPDLFMV
                     RREVLARLVPQGLVAPVDDLYAKMPVRTANHYDEVSRRNAMFNGKSYGLSDPGAIIKN
                     EGLLIRKDWLDNLGLAVPTTIEELFTVMRAFTFNDPDRNGKHDTWGYGSFEEIGSYEG
                     WPGRRLQPIFGAFGAEGTWDMTEANAGLSVFKDGFYDAMVYLKRMVDEGVIDPNWMSY
                     KKDDFRAAWKQGRFGIMREQNAAYAAQSNYAPFDQNFPDGQWIIIDPPKGPKGHASVG
                     PYTANFRVYAISAKAAQAGKKDKIAELLEWMASDEGYYLLGWGVEGVNYTLDANGIPV
                     AAGVPDPDYSFTGPKGQVVTQLRNMVFYNGDVELYARYPTYTTAVSKKQMSALDVLRE
                     MQTKKWTPAVGSNTLPTPNADLKRFYEQSLSEFITGKRTLNRANWNAWLEEFRKLGGQ
                     AWNDAGVASMKENNLLN"
     gene            complement(2822..3724)
                     /locus_tag="PLF67_07080"
     CDS             complement(2822..3724)
                     /locus_tag="PLF67_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89806.1"
                     /translation="MRKKKQVSAIAASTKGDKVFDFLVNLFLVFMLVVIAVPMWSTIT
                     LSFRPNAYIGSNLQGMFLAPWKWSTAAYRALLGNDGFLMAFKNSFKILIGGVATSLFL
                     TIPLAYVLSIQSLPGRRYLTILIIIPYIFNVGLIPTYLLVTKLGLIDKIMAVFLPGAV
                     STYNTLIMRSFFEGIPNELKESARIDGASEIRILVMIILPLSKAIIMTIGLFYGVGFW
                     NDFFRAMLYLNSNKLQPLPILLRNILMASGMNEFVEISAFGDAPIEAIKAASVFMSAI
                     PMIVAYPFIQKYFTKGTLLGSVKG"
     gene            complement(3737..4660)
                     /locus_tag="PLF67_07085"
     CDS             complement(3737..4660)
                     /locus_tag="PLF67_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE89807.1"
                     /translation="MVTKSRKSELSREIRRHASVYVLLIIPLVYYITFKYVPIFNGQI
                     AFKDYMALDGVLGSRWIGLEHFRTFIQSYYFAELLRNTLMYSFGKLIFSLPLAILLAI
                     TIYESKRRRLSRTVQTLAYLPHFLSWVIMYGILLALLAPGDGVVNDIIKAFGGKPRAF
                     LTSVETFPWIVIFSDAWKEMGWSAIIFIAALMGIDPALFEAAMVEGATSWQRVRYITL
                     PSIRPVIVIVVLLRLGTILDAGFNQIFMLYSTPVLSVADIIDTWVYRQGLLEFQFGLA
                     TAVGLFKGAIGMLLILFTNRIVKRLGGSSLY"
     gene            complement(4781..7834)
                     /locus_tag="PLF67_07090"
     CDS             complement(4781..7834)
                     /locus_tag="PLF67_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE89808.1"
                     /translation="MKKIALVLASIHTGSSVTLWKEIAELAEASGDQLFVFPGGRIAY
                     QENQEYLRNEIYALVNPDNVEGMLVWASALSGAVSIEEVEAYLYNEELPTVSIGVKRS
                     AWPCVAFDAYAGMQTVIHHVIETHHVKKIAFLRGPEHHDSAQSRYNAYIDTLQHYRID
                     VDPLLISDPVGWTEGKRAVKQLLDERCLVPGRDFDTLVCSSDLMMFGAGKHLEQLGYI
                     TGRDYIITGFNDSRESRLLKVGCTTARMPVVNLARISYSLLGQNLAGDDSLLYDITLP
                     CPLIIRRSCGCTYALGDEEHARSILVNETRFAEWIIEHLEVTKEEEPLLESFLAGDFG
                     VLSQFARSYFDREGDPNHLTEMIHWYQKFFAKKPFDNERIMGDLLAIQDLVTHEHAYE
                     RDCQEKLLNNLKSDLLTVRRIPALGTIMAQHLPDLGITDAYLMLDGEEGVTTLVGGYR
                     PEGPIRTQEQFSSRLLLPQHYSQALGKGVFVILPLSVDNQSIGYLLVRSTRFNAVVLE
                     DVRTSLSSAIKGALLVESANKARDLAEKTERSHAEFFANISEALRAPLEEILEITSDV
                     TIANHVHHLFNTIDLSLSYTPEFTLEQRTFDPLALIRRLGSVAYSGPSRLPILVGDSV
                     RLGQALEILSGSKEVVLEARLADEGVELFLRIHVEAEAQRDVAMRIIVMNGGQIHFDE
                     HGVRILLPFPSVLGRAKKTSGTRKVYFRGDQEEVLPPFLSELPDLEVVQASGVKSREL
                     EAFSSSTLYWDGSRDDGSLRRLLFQIIAHSELSTCPFVCYDTPVGHETLAVGLAVSKP
                     QEGSLVITGSLGPYFGFLRDAMVVEAREVPKLLQDTVLGLLICDCIDADFLGALRKKT
                     SAPFVLLRDAWSPKEVETLGLIPNVVLAHTSITSNPDFIARLSALRSLGDVLPPLTGI
                     LVKKAVAYIDEHATEGMTRWQLAEAVHVSEDYLTRIFRKEIGVSPWEYINNHRIHKSI
                     DLLKRSTLTIAEIAGKCGFQDQAYFTRVFRKITGKSPTMVRLDGK"
     gene            7909..8895
                     /locus_tag="PLF67_07095"
     CDS             7909..8895
                     /locus_tag="PLF67_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOE89809.1"
                     /translation="MFHFQTLEARLGSLNRLLERFMPLITIGGVVTGLFFHRVFAPLK
                     SFVNPLFAFITFSSALAISPKRFFGALTKIKPIVLFLLWANLVMPLLAFALASLFFTE
                     ANLVTGFVLLTSIPTAMAGYIWSGIYHGDEPLSLVLILISTLLGPILTPLTVKLLAQK
                     AVVIDTRAMIMSLLVMIVLPSLAGILINHAVGDERTKKVTPSLKPFAKIALFAIVTIN
                     ISRIAPTLIERATLAYVPIALLAAFLAFLGYPASHTLGKIARLSLEENKSLTFAASMR
                     NISASMVLAINYFPPITALPIIFGIVFQQSICAIMASVLYGPKRKEAALETG"
     gene            8882..10414
                     /locus_tag="PLF67_07100"
     CDS             8882..10414
                     /locus_tag="PLF67_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HOE89810.1"
                     /translation="MKRVDVKAIRIRPEDTVAVAVHPLKKGETIVIGDFAVTVLEDIG
                     GGHKVALTVTGKGEPVIKYGSPMGVATKNIAVGEHVHTHNTSTRLSEHDEYYYDLNVR
                     DAFQQEADRRAKRREDSIPTIKAFERTDGKIGIRNEIWIVPTVGCVNMTAEQLVKWAE
                     KNLPKNEHYEGTYAWTHPFGCSQLGEDHETTRTILAGLVRHPNAGGVLVLSLGCENNT
                     PESFKELLGEVDPKRVKFLTTQEVEDELEASHALIRELYDEMQNDVRTPVGMDRLIVG
                     FKCGGSDGYSGITANPLVGYFCDELTAMGGTGILTEVPEMFGAEQLLMNRATDETVYE
                     RIVTLINDFKRYFVKHGQVVYENPSPGNKKGGITTLEEKSLGCIQKGGKAIVTDVIPY
                     GGQVHKRGLTLLSGPGNDIVSTTALTAAGAHIILFTTGRGTPLGSPVPTVKIASNTAL
                     AEKKPHWIDFDAERLLREEQDVVVDDFIDMMREIAGGKKLAKNEEHGYREISIFKDGV
                     VL"
     gene            10438..11841
                     /gene="uxaC"
                     /locus_tag="PLF67_07105"
     CDS             10438..11841
                     /gene="uxaC"
                     /locus_tag="PLF67_07105"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOE89811.1"
                     /translation="MKQFMGDDFLLKNKSSERLYHEFAKDQPIYDYHCHLIPGELATN
                     KRFTTITDMWLGGDHYKWRAMRANGIPEAIITGKDADPYDRFLAWAKTVEKLMGNPLY
                     HWTHLELKRYFHIDEPFNEKNAKAVFDETNRQLQNNPNLSVKGILKQFKVYAIGTTDD
                     PADDLGYHKEIAGQKDFPARVMPSFRPDKALAIDAPAFREYLGTLASSANMTITSLDD
                     LVSALIKRLDFFVEMGCRASDHALVTTPALFWSEEKVKKVFAKRLNDESLSKEEVEAY
                     KTYVLTHLAKAYKKRDIAMQLHFAAIRNNNSMMFEKLGPDTGYDASHDKELAEGLSAF
                     LNNLSKSGEVPKTILYTLNPKDYYSLGTLMGCYQDGRPGKMQLGSAWWFADHKDGMEE
                     QMKLLGNVGLLSRFVGMLTDSRSFLSYSRHEYFRRILSNIFGTWMEDGEIPNDIDIIG
                     KIVSDISFNNAKAYFEG"
     gene            11844..12608
                     /locus_tag="PLF67_07110"
     CDS             11844..12608
                     /locus_tag="PLF67_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOE89812.1"
                     /translation="MSEQQGTSGVIRTITILETLSRHQSINLESLAKETKLPKATLLR
                     FLSTLVNLGYVYRDPHDLYSLTLKMFSVGSRGLEHLDLITLARPIAQHLSEELGETVH
                     MGVLEEDNAVYVLKRESSYTIRMYSRVGKTIPLYCTAIGKVLLADMEDEEIASYLERV
                     RLKAFTPNTLTSKEALLSELEEVRTLGRAMDNEEHEMGTLCIGSPIRDHSGKVIAAMS
                     VSWPLFRFDSEQAERMSGAIQEATARLSRLMGYTGG"
BASE COUNT         2841 a         3604 c         3517 g         2773 t
ORIGIN      
        1 aatatcgccc cgcggagggt gctttataag ggaaagcaga atcccctccc agggcgaaga
       61 tcatccaatc gttacgttga aagcgtttcg cgctccaagg ttgccagaat gtaggggccg
      121 acacccttga agtcatcggc gacttgcggt tctttgaagt agtagtagag agaaccgtcc
      181 cgataggggt ttcctccgag acccgccacg ctgcagatac cttcgagatg atactccccg
      241 tcttcatcga tgctcaaata gcgtttttcg atcccgtcca gcgccttgag ggcggatttt
      301 tgaatcttgg gatcggcgcc gattcccaac ctcgccatct tcagaagcgt atatgcgaac
      361 atcgcgctcg ctgaagtctc cttgtagtta ccttcagctt cgcttcggtc gacaacctgg
      421 tagaacatcc cgtcgggctg ttggaatgcc agaatatcgc ccatcagcgt attgaggatc
      481 ttcttcagcg gatcggaaag cggatcatcg gttgaaatga tcgctatgat gtcaacgacc
      541 gccatcgcat accacccgag cgcccttccc cagaaatggg gagaaagccc cgtttcgagg
      601 tcggaccagc gcattccccg cgattcgtcc catgcatggt agagcagtcc ggttttggaa
      661 tcccgaagtg tcgcatatat cctttcaatt tggattgcga gatcttcgat gatttcacgc
      721 tgatcgacat acttagcata gagggcggag aacggtccct gcatatagac tccatccagc
      781 cagacctgcc acggatagat ctgcttgtgc cagtagatcc cgctgcgcgt cctcggctgg
      841 ccgaccagct gttcgcgaag gatgtgggcc gccttgatga accgccgttc tccggttttc
      901 tcgtgcagga gaaaaagatt ccgtccggca ttgatcatat cgaggttgta ctcgcccgct
      961 cgatagtcct tgatcgttcc atcctccttg atgaacgggt catacatcga atacgcccaa
     1021 tcgaagaagg atgtttgtcg atagtgctca cccacctcca agattgcggt gatgaccaat
     1081 ccatgctcat aatgccactg catctgagcc ggttggtacc gattgatgac gcttttagcc
     1141 atttccaagg agtagttctt tgccataatc caattccaag agcacgagtc cgaccggatc
     1201 ggccggactc atggcacatc tcctagttca ggagattgtt ttctttcatg gatgccacgc
     1261 cggcatcatt ccacgcttgt ccgccaagct tcctgaactc ttcaagccag gcgttccagt
     1321 tcgcccggtt cagcgtgcgc ttgccggtga tgaactcgct gagcgattgc tcgtagaagc
     1381 gcttcaggtc ggcattgggg gtcggcaggg tattggagcc cacggcgggg gtccacttct
     1441 tggtctgcat ctccctcagc acgtcgaggg cgctcatctg ctttttgctc accgcggtgg
     1501 tataggtcgg atatcgggca taaagctcga cgtcgccgtt gtagaagacc atgttgcgca
     1561 gctgggtaac aacttgaccc tttggtccgg tgaagctgta gtcgggatcg ggaacaccgg
     1621 cggcaacggg aattccattt gcatccaagg tgtagttcac cccttcgaca ccccagccaa
     1681 gaaggtagta tccttcatca ctggccatcc actccaagag ctcggcgatc ttgtccttct
     1741 tgccggcttg ggcggctttc gcactgatcg catagacgcg gaagttggcg gtgtacggtc
     1801 cgaccgaggc gtggcctttc gggcccttcg gaggatcgat gatgatccac tgtccgtccg
     1861 ggaagttctg atcgaaggga gcatagttgg actgagccgc atacgccgcg ttctgctcgc
     1921 gcatgattcc gaatcggccc tgtttccagg cggctcggaa gtcgtccttc ttgtagctca
     1981 tccagttggg atcgatcaca ccttcgtcga ccatccgctt caggtagacc atcgcatcgt
     2041 agaagccgtc cttgaagacc gacagtccgg cgttcgcctc cgtcatgtcc catgtgccct
     2101 cggcaccgaa agcgccgaag atcggctgca ggcgccgccc gggccaccct tcgtagcttc
     2161 cgatctcttc aaagctgccg tagccccacg tgtcgtgttt cccgttccga tcgggatcgt
     2221 tgaaggtgaa cgcccgcatc acggtaaaca gctcttcaat ggtggtggga accgcaagtc
     2281 cgagattgtc cagccaatcc ttccgaatca gcaatccttc gttcttgatg atcgctccgg
     2341 gatcggaaag accgtagctc tttccgttga acatggcgtt tcggcggctg acttcgtcat
     2401 agtggttcgc cgttcgaacc ggcatcttcg catagagatc atcgaccggg gctaccagcc
     2461 cttggggtac gagccgggca agcacctccc ggcgaaccat gaagaggtcg ggaagattgt
     2521 tcgccgccgc tgcagcttgg atcttcacat cctggtcgcc ttcgctggaa ggaagggcgg
     2581 tgatcttcaa atcgatgttg agtttgtctt tgatgatctg aaacccaacc cagtcctctg
     2641 ggggaggtcc tgcctccgtc atcgccgcac cgtaccacag ctcaatgacg gtcgttcctt
     2701 cacttgcctt tttgtctgca gctccctgac tgaagagcgg catcgccgcc aaagccaaga
     2761 tcgtcacaag gactaaccac gtactcattt gttttctcat cgaaaacctc cttattccac
     2821 atcatccttt gacacttccg agcaacgtgc ccttcgtgaa gtatttttgg atgaacgggt
     2881 atgcaacaat cattggaatc gcgctcatga agacgcttgc cgctttgatc gcctcgatgg
     2941 gagcatcgcc gaacgcgctg atttccacaa attcgttcat gccgctcgcc ataaggatgt
     3001 tgcgcagcag aatcggcagc ggctgaagct tattgctgtt gaggtagagc atcgcccgaa
     3061 agaaatcgtt ccagaacccg accccgtaga agaggccgat cgtcatgatg atcgcctttg
     3121 aaagagggag gatgatcatc accaggatgc ggatttcact cgccccgtcg attcgggccg
     3181 actccttcaa ttcattcgga atcccttcga agaaggagcg catgatgagg gtgttatagg
     3241 tgctgaccgc tcccggaaga aacaccgcca tgatcttgtc gatgaggccg agtttcgtca
     3301 ccagaaggta ggtcggaatc agtccgacgt tgaaaatgta cgggatgatg atcaggatcg
     3361 tcagataccg gcgtccgggc aggctctgga tcgaaagcac atatgcaagc ggaatggtaa
     3421 ggaagaggct ggttgcgacc cctccgatga ggatcttgaa ggagtttttg aacgccatca
     3481 gaaatccgtc atttccaagc agcgctctgt aggcggcggt cgaccacttc cacggagcaa
     3541 ggaacatccc ctgaaggttg ctgccgatat acgcattcgg tctgaaggaa agggtgatcg
     3601 tcgaccacat gggaactgcg atcacgacca acatgaagac caggaagagg ttgacgagga
     3661 aatcaaagac tttatctccc ttggtggagg ctgcaattgc tgatacttgt tttttcttgc
     3721 gcatatgaac cgcctcctag tagagtgatg aaccgccaag ccgcttgacg atgcggttgg
     3781 tgaagagaat caacaacatt ccgatcgcgc ccttgaagag tccgacggcg gtggcgagtc
     3841 cgaattggaa ctcgagaagt ccctggcgat acacccaggt gtcgatgatg tccgccacgg
     3901 agaggaccgg ggtcgaatag agcatgaaaa tctggttgaa cccggcgtca agaatcgtcc
     3961 ccaggcggag gagaaccacg atcacgatga ccggccgaat cgaaggcagt gtgatatacc
     4021 ggacccgctg ccacgaggtg gccccttcga ccatcgccgc ctcgaagagc gcggggtcga
     4081 tccccatcag cgcggcgatg aagatgatcg cgctccagcc catctccttc cacgcatcac
     4141 tgaagatcac gatccaaggg aacgtttcga cgcttgtcaa aaatgctcgg ggtttcccgc
     4201 caaacgcctt gatgatgtcg ttcaccaccc cgtctcccgg agcgagcagg gcaaggagga
     4261 ttccgtacat gatgacccag gaaaggaagt gcggaagata tgcgagggtc tggactgttc
     4321 tggagagccg ccgtcgtttt gattcataga tcgtgatcgc cagaagaatc gccagcggca
     4381 ggctgaagat gagttttccg aagctgtaca tcaacgtgtt gcgcagcagt tcagcaaaat
     4441 aataggattg gatgaacgtc ctgaaatgtt cgaggccgat ccaccggctt ccaagcactc
     4501 cgtcaagagc catgtagtcc ttgaaggcga tctgaccgtt gaagatcggt acgtatttga
     4561 aggtgatgta atacaccagc gggatgatga gcaggacata caccgaggca tgacgacgga
     4621 tctcccggct caattcactc tttcttgatt ttgtaaccat ctgctttccc cttacctgaa
     4681 gggtatggca tctcaagcag attatgcaga ggggctcttg tacttttccg acattttctc
     4741 cattcaccga ccttgcattc ggggatcgat tgattgcttc ctacttgccg tccaagcgaa
     4801 ccatcgtggg gctttttccg gtgatcttcc gaaacacccg ggtgaaatat gcctgatcct
     4861 gaaaaccgca cttgcctgcg atctcggcga tcgtgagggt cgaacgcttc aggagatcga
     4921 tcgacttgtg gatccgatgg ttgttgatgt actcccacgg ggagaccccg atttctttac
     4981 ggaagatccg ggtcaggtag tcttcgctga cgtggaccgc ttcggcaagc tgccaccgag
     5041 tcatcccttc ggtggcatgc tcgtctatat atgcgaccgc cttctttacg aggattcccg
     5101 tcaagggagg aagcacgtcg ccaagcgaac gaagcgccga gagccgggcg atgaaatccg
     5161 ggtttgaggt gatggaggta tgggcaagca ccacgtttgg gatgagaccg agcgtttcaa
     5221 cctccttcgg cgaccaagca tcccgcaaga ggacgaaggg agcgctcgtc ttctttcgaa
     5281 gggcgccgag gaaatccgca tcaatgcagt cgcagatcaa gagacccaac acggtgtctt
     5341 gaaggagctt cggaacctcc cttgcttcca cgaccatcgc atcacgcagg aatccgaagt
     5401 acggacccaa tgaaccggta atgaccaggg aaccctcctg gggcttcgag accgccaagc
     5461 ccaccgccaa tgtttcgtgt ccgacgggcg tgtcgtagca cacgaaggga caggtgctca
     5521 attcggaatg ggcgatgatc tgaaagagca gccggcgcag agagccatca tcccgcgaac
     5581 cgtcccaata gagcgttgaa gagctgaagg cttcaagctc gcggctcttc acacccgaag
     5641 cctggaccac ctcaagatcc ggaagctctg aaagaaacgg gggaaggacc tcctcctggt
     5701 cgcctcggaa atatactttc cttgtgccgc ttgtcttttt cgcccgcccg aggacgctcg
     5761 gaaagggaag aagaatccga acgccgtgct cgtcaaaatg gatttgtcca ccgttcatca
     5821 cgatgatccg catcgccacg tctcgctgcg cctccgcttc gacgtggatt ctgaggaaca
     5881 attcaacccc ctcatccgca aggcgagcct caaggacgac ctccttggag ccggagagga
     5941 tctcaagggc ttggccgaga cggacggagt ctccgacaag aatcggcagc cgggaaggcc
     6001 cgctgtaggc aacggaacca agccgtcgga tcaacgcaag aggatcgaac gtccgctgct
     6061 ccaaggtgaa ctcgggggta taggaaaggc tcagatcgat cgtattgaag aggtgatgaa
     6121 catggttggc gatcgtcaca tcgcttgtaa tttcaaggat ctcttccaaa ggagcccgaa
     6181 gcgcctcgct gatgttcgcg aagaactcgg catggcttcg ctccgtcttt tccgccagat
     6241 cccgggcttt gttggcgctc tcgaccagaa gcgccccctt gatcgcgctg ctgagactgg
     6301 ttcgcacatc ttccagaacc accgcgttga accgggtcga ccggaccaac agatatccga
     6361 tgctctggtt gtcgacggaa agcggaagga tgacgaacac ccccttgccc aatgcttgac
     6421 tgtagtgttg agggaggaga agccgactcg aaaactgctc ctgcgttcgg atcggaccct
     6481 ccggtcggta ccctccgacc aaggttgtca ctccttcttc cccatccagc atcaagtatg
     6541 cgtcggtgat tcccagatcc ggcaggtgtt gagccatgat cgtcccgagg gccggaatcc
     6601 ggcgaacggt caacagatcg cttttgagat tgttcagcag cttctcctga cagtcgcgct
     6661 cataggcgtg ctcgtgggtg accagatcct gaatcgccaa taagtcgccc atgatccgtt
     6721 cgttgtcaaa cggtttcttg gcgaagaact tctgatacca atggatcatc tcggttaggt
     6781 ggttcggatc cccttcccga tcgaaatagg agcgggcgaa ttgcgaaaga acgccaaaat
     6841 cccctgccag aaaggattcg agaaggggtt cttcctcctt cgtcacttcc aggtgttcga
     6901 tgatccactc ggcgaaccgg gtctcgttca ccagaatcga gcgggcgtgt tcctcgtccc
     6961 cgagggcata ggtgcagccg caggagcggc gaatgatcag cggacaggga agcgttatat
     7021 catagagaag cgaatcgtcg ccggcgaggt tctgcccaag cagggagtag ctgatccggg
     7081 caaggttcac caccggcatc cgggcggtgg tgcagccgac tttgagaagg cggctctccc
     7141 ggctgtcatt gaagccggtg ataatgtaat cgcgccctgt gatgtaaccc agctgctcaa
     7201 ggtgctttcc ggcgccgaac atcatcaggt cgctggagca gacgagcgta tcgaaatctc
     7261 tgcccgggac cagacagcgc tcatcgagca gctgcttgac cgcccgtttt ccctcggtcc
     7321 agccgaccgg atcgctgatc aagaggggat cgacatcgat tcgatagtgt tgaagcgtgt
     7381 cgatatatgc gttgtagcgg ctttgggctg aatcgtgatg ttcgggaccc cgaagaaagg
     7441 cgatcttctt cacgtggtgg gtttcgatca catggtggat caccgtttgc atccccgcat
     7501 acgcatcgaa cgccacgcag ggccaggcgc tgcgtttcac gccgatcgat acggtgggca
     7561 gctcttcatt gtagagatac gcctccacct cttcgatcga taccgcaccg ctgagggcgc
     7621 ttgcccagac cagcattcct tcgacgttgt cgggattgac cagagcatag atctcatttc
     7681 gcagatactc ctggttctcc tgataggcga tccgtccgcc ggggaagacg aagagctggt
     7741 cgcccgaagc ttcagcgagc tcggcgatct ctttccacaa cgttacggag gatccggtat
     7801 gaatcgaggc aagaacaagg gctatttttt tcatcttctc atccccgact ataccatgcc
     7861 caatgtgtat cttgacagta ttcggcagcg cttatatgct ctgaaacaat gtttcatttc
     7921 caaacccttg aggctcggct cggctctttg aataggctgc ttgaacgatt catgcccctc
     7981 atcacaatcg ggggcgtggt taccggtctg ttcttccatc gggtgttcgc ccccctgaaa
     8041 tcgttcgtaa atccgctgtt cgccttcatt acgttttcca gcgcattggc gatcagtccg
     8101 aagcggtttt tcggggcctt gacaaaaatc aaaccgatcg ttctttttct gctatgggcg
     8161 aacctcgtca tgccgctact cgctttcgct ttggcgagcc tcttcttcac cgaagcgaac
     8221 ctcgtcaccg gcttcgtcct tttgacatca attcccacgg cgatggccgg atacatctgg
     8281 tcggggatct accatggcga cgaacccctt tccctggtgc tgattctcat ctcgacgttg
     8341 ctgggaccga ttcttactcc gctgaccgtg aagctgctcg cccaaaaagc cgttgtgatc
     8401 gacacgaggg cgatgatcat gagcttgctg gtgatgatcg tccttccctc cttggcggga
     8461 atcctcatca accatgccgt aggggatgag cgaacgaaga aggtgacacc ttctttgaag
     8521 ccgtttgcaa agatcgctct ctttgcgatc gtgacaatca acatctcccg gatcgccccg
     8581 acgcttattg agcgggcgac gcttgcctac gttccgatcg ctctgctcgc cgctttcttg
     8641 gcgttcctcg gctatccggc gtcccataca ctcggaaaaa tcgcccgtct ctcccttgaa
     8701 gaaaataaaa gcctgacatt cgccgcttcg atgcggaata tcagcgcttc catggtgctc
     8761 gcgatcaact atttccctcc gataaccgcc cttccgatca tcttcggaat cgtctttcag
     8821 caatcgatct gtgcgatcat ggcgtccgtg ctctatgggc cgaaacgcaa ggaggccgca
     8881 cttgaaacgg gttgatgtga aagcgatcag gatccgtccc gaggataccg tagcggtagc
     8941 ggttcatccc ctcaaaaagg gagaaacgat cgtcatcggg gatttcgccg tgaccgtgct
     9001 cgaggatatc ggtggaggtc acaaggtcgc attgactgtg accggaaagg gagaacctgt
     9061 catcaaatac ggttctccga tgggtgtcgc caccaagaat atcgcagtcg gtgaacacgt
     9121 gcacacccat aacacatcga cccgcctgag cgaacatgat gagtattact atgacttgaa
     9181 cgtccgcgat gcgttccaac aggaggcgga ccggcgggca aagcgccggg aagactccat
     9241 tccgacgatc aaggcctttg aacggaccga tgggaagatc ggcattcgca acgagatctg
     9301 gatcgttccc acggtggggt gcgtgaacat gaccgccgaa cagctcgtca agtgggcgga
     9361 gaagaatctt cccaaaaacg aacactacga gggaacctat gcgtggaccc accccttcgg
     9421 atgctcccag ctcggtgagg accatgagac gacccgaacc attttggcgg gcttggtccg
     9481 ccacccgaac gccggcggtg tcctggtcct ctccctcggc tgtgaaaaca acacccccga
     9541 gtccttcaag gagctcctcg gcgaggtgga tcccaagcgg gtgaagttcc tcaccactca
     9601 ggaggtggag gatgagcttg aagcctccca tgccctcatc agggagctgt atgatgaaat
     9661 gcagaacgat gtccggacgc ccgtggggat ggaccggctc atcgtcgggt tcaaatgcgg
     9721 aggatccgac ggatactccg gcatcaccgc aaaccccctg gtcggatact tctgtgatga
     9781 gctgacggcg atgggcggaa ccgggatcct caccgaggtt cccgagatgt tcggtgcgga
     9841 gcagctgttg atgaacaggg cgaccgacga gacggtttac gaacggatcg tcaccttgat
     9901 caacgacttc aaacggtatt ttgtcaaaca cggtcaggtc gtgtatgaaa acccctctcc
     9961 cggaaacaag aaaggcggaa tcacgacgct tgaggagaag agcctcggat gcatccaaaa
    10021 aggggggaaa gcgatcgtca ccgacgtcat tccctacggc ggtcaggtac ataaacgggg
    10081 actgaccctt ctttcaggac cgggtaacga tatcgtctcc accaccgcct tgacggccgc
    10141 cggagcgcac atcatcctct tcaccaccgg tcggggaacc cctctcggct cccccgttcc
    10201 gacggtgaag atcgcctcca acacggcttt ggcggaaaag aagccccatt ggatcgattt
    10261 tgacgcggag cggctgctcc gggaagaaca ggatgttgta gttgacgatt ttattgatat
    10321 gatgagagag atcgccggcg gcaagaagct tgcgaagaat gaagagcacg ggtatcgtga
    10381 gatttcgatc tttaaagacg gggtggttct gtaaccccat acaaaggagt tcctgcgatg
    10441 aaacagttta tgggagatga tttcctcttg aaaaacaaga gttccgagcg gctctaccac
    10501 gagttcgcga aggatcagcc gatctacgat tatcactgtc acttgatacc gggtgaactg
    10561 gcgacgaaca aacgcttcac cacgattacc gatatgtggc tgggcgggga ccactacaag
    10621 tggcgggcga tgcgggccaa cggaattccc gaggcgatca tcacgggaaa ggatgccgac
    10681 ccgtatgaca ggttcctcgc ctgggcgaaa acggtcgaaa agctgatggg gaatccgctc
    10741 taccactgga cccatctgga gctgaagcgc tacttccaca ttgatgagcc gttcaacgaa
    10801 aagaatgcga aagcggtatt cgacgagacg aaccgccagc ttcagaacaa cccgaatctt
    10861 tcggtcaagg ggatcctcaa gcagttcaag gtctacgcaa tcggaacgac ggacgatccg
    10921 gcggatgacc ttggctacca caaggagatc gccggccaga aggatttccc cgcccgcgtc
    10981 atgccttcct tccgccccga caaggcgctc gccatcgatg ctccggcctt ccgggagtat
    11041 ctgggcacgc tcgcttcttc ggcgaacatg acgatcacga gccttgacga tctcgtctca
    11101 gcgttgatca agcggctcga cttcttcgtc gagatgggct gccgagccag cgaccacgcc
    11161 ctggtcacca ccccggcgct cttctggagc gaggagaagg tcaagaaggt cttcgccaaa
    11221 cgcctgaacg acgaaagcct ctccaaggag gaggtcgaag cgtacaagac ctatgtgctc
    11281 acccaccttg caaaggcgta caagaagcgg gatatcgcga tgcagctgca cttcgcggcg
    11341 atccgcaaca acaacagcat gatgttcgaa aagctcgggc cggacaccgg ctacgacgct
    11401 tcccacgaca aggagctcgc cgaggggctt tcagcgttcc tcaacaacct ttccaagagc
    11461 ggcgaggttc ccaagaccat cctctacacg ctcaacccca aggattacta ctccctcggc
    11521 acgctgatgg gctgctatca ggacggtcgg cccggaaaga tgcagctcgg ttcggcgtgg
    11581 tggttcgccg accacaagga cgggatggag gagcagatga agcttttggg aaacgtcggt
    11641 cttctctccc gcttcgtcgg aatgctgacc gacagccgct ccttcctctc ctactcccgg
    11701 cacgagtact tccgaaggat tctttccaat atcttcggaa cctggatgga agacggtgaa
    11761 attcccaacg atatcgatat aattggcaag atcgtcagtg atatctcgtt caacaatgcc
    11821 aaagcctatt tcgagggatg atgatgagtg agcaacaagg aaccagcgga gtaattcgaa
    11881 cgatcacgat tctggagacg ctttcgcgcc accagtcgat caatctcgag agccttgcca
    11941 aggagacaaa gctcccgaag gcgactcttc tgcgttttct ttcaacgttg gtgaaccttg
    12001 gctatgtcta tcgcgacccg cacgacctct acagtctgac gctgaagatg ttcagcgtgg
    12061 gttcgcgcgg cttggagcac ttggatctga tcactctcgc ccgcccgatc gcccagcacc
    12121 tcagtgaaga gctgggcgag acggttcaca tgggagtcct cgaagaggac aatgcggtgt
    12181 atgtcctcaa gagggaatct tcctatacga tccggatgta cagccgggtg ggaaaaacca
    12241 ttccgctcta ctgcacagcg atcgggaagg tgctcctcgc cgacatggag gatgaagaga
    12301 tcgcctccta cctcgaacgg gtcaggctca aagcgttcac tcccaacacc ctcaccagca
    12361 aggaagccct gctttccgaa ctcgaagagg tccggactct cggccgggcg atggacaacg
    12421 aagagcacga gatggggaca ctgtgcatcg gttctccgat ccgcgaccac agcggaaagg
    12481 tgatcgcggc gatgagcgtc tcgtggccgc tcttccgctt cgattccgaa caggcggaac
    12541 gaatgagcgg ggcaattcag gaagcgaccg cccgcctctc gagactcatg ggatatacgg
    12601 gcggatgata tcctccatcc ccctctcttc tccgtttaca tgagtcggat atgctttcct
    12661 taacatcggt gggtcgcttg caagcaaaca agggattgtc accacatagt aaggtaagat
    12721 gatggcagca ttctt
//
LOCUS       DAOSLI010000047        12307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_5174_length_12307_cov_29.644874, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000047 DAOSLI010000000
VERSION     DAOSLI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5174_length_12307_cov_29.644874"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLF67_07115"
     CDS             complement(<1..407)
                     /locus_tag="PLF67_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE89813.1"
                     /translation="MNVLEALEKELIKVPLESSEKYAIIRELVDVAAQAKGYDGEALL
                     DAVLARERLGSTGIGNGIAIPHAKSDAVNKVTMVIGVSKIPVDFESIDNQKARIFFLV
                     IAPSKEASAHVELLASIARSCSSGIFRRMLEQA"
     gene            complement(420..902)
                     /locus_tag="PLF67_07120"
     CDS             complement(420..902)
                     /locus_tag="PLF67_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE89814.1"
                     /translation="MSVPFKQIVAYIDGSESSMTALMYAIKLAKEHHARATAVYVVNT
                     KALSDLVKSGIFVAVERDEYQRDLYEDADRYLRHAQHLAEEKGVEIDTVKLEGSVHAV
                     VKELVKSLGADLLILGGVSDIRSRRDELASETDRMMRTAPCPVLVVREDDDIWDEFER
                     "
     gene            complement(904..1587)
                     /locus_tag="PLF67_07125"
     CDS             complement(904..1587)
                     /locus_tag="PLF67_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOE89815.1"
                     /translation="MATTTLAPTRTNLLKLSEDLKFAKLGHELLDQKRSILVVELLTL
                     VDQAVDFETRVLSSLKEAQNALSDAIMQMGRLRVGNLAGAVNIDYSIRVGSRRVMGVS
                     VPKVETSFIDKSPYFSSEETSILSEVAIDRYRSALTLMGHLAELKVSIMRLAREVKKT
                     IRKVNALEKIVIPRTEETIAWIDSRIEEQDRENVIQLKTIKDRMERIKALNQVLTQTD
                     DDDTITTGV"
     gene            complement(1589..2992)
                     /locus_tag="PLF67_07130"
     CDS             complement(1589..2992)
                     /locus_tag="PLF67_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOE89816.1"
                     /translation="MNINISQSNLLSGREYRGAARIDGPLVTMRNTHNVGYGELVELS
                     TGDGKIRLGQVLDTSDEIVVVQVFEGTDGLSMPGTGMRFLGEPLTLAVSEQMLGRVMN
                     GLGKSRDGSATPQGYAERDVNGEPINPTAREYPRDFIQTGISVIDGLTTLIQGQKLPI
                     FSGSGLSHNQLAAQIARQAKVRGSGGDFATIFVAMGVKYDVAKYFIDSFEESGVLDNV
                     ALFLSLADDPSIERIITPKTALTLAEHLAFDKGKHVLVIMTDMTNYCESLREIGTLRG
                     EIPSRKGYPGYLYSNLAEIYERSGKIIGRSGSITQLPILSMPNDDISHPVPDLTGYIT
                     EGQIVFERGMHARGLYPPINPLPSLSRLMKDGIGEGMTREDHPHLANQLFASYSHVKD
                     VQNLASVIGEEELTALDQQYLEFGTFFENRFINQGYDEDRSIEETLDLGWEALSKLPS
                     EELQRVTDEEIAQYYRG"
     gene            complement(2982..4760)
                     /locus_tag="PLF67_07135"
     CDS             complement(2982..4760)
                     /locus_tag="PLF67_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOE89817.1"
                     /translation="MNERIIGKIKRVNGPVLIVKGIQDARMMELVHIGEQRLVGEVVK
                     LHDGEATVQVYEDATGLRPDDNVYGSGMSLSVEVGPGLIGQIYDGIQRPLEELMGLSG
                     AFIARGLSFDAISHIKQWEFTPVIKKGASVSRGMILGTVQETNQIEHRIMVPLTIEEG
                     IVTEIVKKGSYTVVEPIAKITLPGGRVVDVPMVQRWPIRVPRPTKERLSLDRPLITGL
                     RVIDTLFPLAKGGTVAIPGGFGTGKTMTQHSIAQWCDADIIVYIGCGERGNEMTDVLR
                     EFPQLIDPRTGNSLMDRTILIANTSNMPVSAREASIYTGIALAEYYRDQGYHVAIMAD
                     STSRWAEALRELSGRMEEMPAEEGFPAYLPTRIAQFYERAGYMKTLSDREGSVSIIGA
                     VSPPGGDFSEPVTQHTRRFVRCFWGLDRGLASSRHYPAISWLDSYSEYLDDVRHWWSE
                     HSEDAWYRNRQKIMDLLQREVRLQQIVKLVGPDALPDTQNFILEVCSLFKTAFLQQNA
                     FDEVDRYCPVAKQVKMLGVILAYYEKGSEAINGGVPMVKLRRLRAVQDMARMRFTIKA
                     DELHQIDRIQLRMERAIDELGGIYEH"
     gene            complement(4757..5353)
                     /locus_tag="PLF67_07140"
     CDS             complement(4757..5353)
                     /locus_tag="PLF67_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOE89818.1"
                     /translation="MKSTETPLLEGILKEAARSAQDHLEDARTQAKKILERAQSRAEE
                     EVASQKRATDEKVKQIQLRLATNKASAKRKAALRQVDDSYQRVMDAVFVALDCKTLKP
                     HLPYWIAEAAIGLDRKEAKVAYSRLCPVTEEDLKKAEALILKATGAKIRLHLEEKYIQ
                     GLGVVLSSIDGSTSFNNQVDIRLRRFERLIRSMIQERA"
     gene            complement(5364..5666)
                     /locus_tag="PLF67_07145"
     CDS             complement(5364..5666)
                     /locus_tag="PLF67_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOE89819.1"
                     /translation="MRYVVIGDEDTVLGLSLVGVFGMQATNPEEARIAWEKALEDKEN
                     GIIIITDEVANMIRSSVDRYLFSETFPLVVEIPSPKSGTKRSDLRALVNQAIGVSL"
     gene            complement(5669..6022)
                     /locus_tag="PLF67_07150"
     CDS             complement(5669..6022)
                     /locus_tag="PLF67_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HOE89820.1"
                     /translation="MTKSMFRRKVTMSLIATALVLVGSGFIFAPSAYAATTEAVATTG
                     LDYNLALVAFAAALAFSVGAISAGMAIGRVGSAAMGAMSERPEIGTQALIFIALAEGV
                     VVFGFITSLMILAKV"
     gene            complement(6051..8042)
                     /locus_tag="PLF67_07155"
     CDS             complement(6051..8042)
                     /locus_tag="PLF67_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOE89821.1"
                     /translation="MNLFTKPMRLLTAVVLQQRSDQVVKALLQLGVLDFIHISRLEAD
                     QLAKLSSKPSTTNRVVLEDLRGRVEALLKQGHLALPGESTLDVSLLAEPQVDAYRAVL
                     DELTQTLLALKERQKVANQALLGVEEMRRYLAESKWQYLDLRVGSVKEGKSEQLEEKL
                     SPYGAFVEPIDEEFVTVLSLRRDLAQIDPLLEKFGWTEESDPLLQKQAVVLVRTRLET
                     EHQKLIDKRGEIEQSVKRAIAERKEELHAIWSNLRLNELSDQIKSYFSYTRNTTLFSG
                     WVPADQAESVRNAIVEASGGQCVVEWTEATEVPRESVPVAVTSPKVLEPFQNMVNNYA
                     TPEYGTVNPTIFVMIAYLSMFGLMFGDVGQGLVLLLVGLFGSRSYRRNPEKKEGLLTR
                     NVTNLLVYLGLSSMLFGALFGSYFGLPLLPPLWFNFEAAVEGHAPATALVRDVYGILG
                     ITIRFGIIIIYTGLVLNWINLFRKKSFVPMLLDKNGVVGGVLFGTGIYMAFGFVGSSY
                     RSFPSDPWIIPTLVICLILLFAKPYVQHFVDLKQGRADKTFGQVAAGSIMEFLVDVLE
                     IFTGYLSNTLSFMRVAGLGIAHASLMGTFKYLQSMVGGVAGVLIFIAGNLLVIVLEGL
                     SAGIQSLRLNYYEFFSRYFTGRGIAYRPVGLKRSSSADR"
     gene            complement(8039..9097)
                     /locus_tag="PLF67_07160"
     CDS             complement(8039..9097)
                     /locus_tag="PLF67_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOE89822.1"
                     /translation="MSSVDTYGFVNAKMRARLGKAREEAVTEALLRAPTLVDAIAALR
                     PTRYAALARSYDQSGDIQSIELVLLKMEIALYREIAQYLDGVEGRFVAELLGKIEIDN
                     LKNSIRLWYSSIIHRRPIRHRSEYLFKEVILHPVDWTALINALSWDGVVEATANTPYH
                     PILPEYTEESLREEGLFQLESRLDRVWYEEIRKATAWLKKTDRQKALELLDQEIDLKN
                     LLILVRYGWYHRLEAKELLSLLLPWGEVFKSKEVNRYIETPKENRSASELLSQFLPDE
                     QRVGDHSAVEEVLRIEELLAQNQKRVYRRLLGGDPFTIGVALAYFFLNKEEFRRIKAI
                     LNGKYYGFDEGYIRGVIG"
     gene            complement(9097..9423)
                     /locus_tag="PLF67_07165"
     CDS             complement(9097..9423)
                     /locus_tag="PLF67_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89823.1"
                     /translation="MRSDIINEVLTVEDRAQQIVRDAEREAREIITNAQTEANAFVRD
                     ALKQDRILAQETIAQAEADLVDQLTEYEASLDRSSRLTAEEVKEIAQAIVIEVCKTNL
                     DGISES"
     gene            complement(9503..>12307)
                     /locus_tag="PLF67_07170"
     CDS             complement(9503..>12307)
                     /locus_tag="PLF67_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89824.1"
                     /translation="PADDDFWADFFVVGEDSAVAYDDGTYYFRLFVNEDLVGTIEVVF
                     EGEERLVNVEDLQFYVAEHLTMAARIRIFSDLGSHVTPEELERRGVETVYDEQAFVLY
                     LTFSIQDMPERILSLSSATVNRREQYAISGAIPLKPRWFSLAANLNLFGQLEYPDDFS
                     KFNRTLLNLSVSNRASLFGVALNYNFTLSHQEPYYNPGSWNGYYDFVDASIRLSFGNI
                     GSGLGKILGAQSSIGISLEKNYAFGTTGAKRTQIEETLTIVEESDVEIFLNDKSFYTR
                     RLKPGVYRLRDFILVQGANRIRIEVTPTSTGKPEKPIYLNFGYDYRLLAKGDTTYAIG
                     LSMPQSRGPNKEGSFALPWLGGDYLSYHPKAFTATYRQELGVTDALTTSIDLAFTPGT
                     LQASLGIVFATMIGSTQIQGSAHVETDTWKASYNMNLGQRFGLSETSFLNRLGLSGNY
                     NRPVDSKGSASVSLSYSDSLFNLLRYSLSGSGAYTFDTSLATWSVSASTGFSPFKGFS
                     INGSISLTGSSNTSKPILTGQISGSYSIGSKASMSNTNSIQATGVNSNLSMSLRPSKD
                     DSITLSYSGVRTEFDGSPPKFGAGVLAGSWNHSGTFSSLNLRHQYSLAQKNMTTTVMI
                     NTALAYAGGAFGLGRSVSDSFLLVKPTGLLKRGQVSVARSLDSSPTYLPRPFGSALYN
                     GLSTNTTNTVAVFSSGPTEFSTGSSFVYSLSPRGRESFVVKISMQPAFTVSGVLIQKD
                     GTPYIQYSSPVYRITTNEEGEEKHDRDDTLYLFTDQDGRFILNEVKAGSYLFDLKVDD
                     DWYGVRFDVPEQDAKTMGINTILLLESYQVDDPAFEGRVSYFDKVAVLLEAQIEDAFH
                     VEVADEYDARLTLNVIEQTDEESFWNAIFPSFDEDPFFSFDDAFVTDDDFYFDPLVFD
                     EMIGDWESPPTEQTVTTAAP"
BASE COUNT         2650 a         3529 c         3308 g         2820 t
ORIGIN      
        1 ttcgcctgtt cgagcatccg gcggaagatt ccgctggagc aggatcgggc gatcgaagca
       61 agcagttcga cgtgggcaga ggcctccttc gagggagcga tgacgaggaa gaagattcgg
      121 gctttctggt tgtcgatcga ttcgaaatca acgggaatct tggagacgcc gatgaccatc
      181 gtgaccttgt taacggcatc gcttttagca tggggaatgg caatgccgtt accgattccg
      241 gtcgatccca agcgctcgcg cgccaaaacc gcatcaagca acgcttcgcc atcgtagccc
      301 tttgcctggg ctgcgacatc aaccaattct cggattattg catatttctc actcgactcg
      361 aggggaactt tgatcaattc tttctctaat gcttccagta cattcatagg tgtcgctcct
      421 cagcgctcaa actcatccca aatatcatca tcttcccgaa caacgagaac cggacacggg
      481 gcggttcgca tcatccgatc cgtctcgctg gccagttcat cccggcggga gcgaatatca
      541 ctcacaccgc ccaaaatcaa cagatcggct ccaaggctct tgaccagctc ctttacgaca
      601 gcgtggaccg acccctccaa cttaacggta tcgatctcca ctcccttctc ttcggcaagg
      661 tgttgggcgt gccgaagata gcgatcggca tcttcgtaga gatctctctg gtattcatca
      721 cgctccactg cgacaaagat tccgctcttg accaaatcac ttagtgcctt ggtattgacg
      781 acatataccg ccgtcgcccg ggcgtgatgc tcttttgcca gcttgattgc atacatcaaa
      841 gcggtcatcg aactctccga cccatcaata tacgcaacga tttgtttaaa cgggactgac
      901 atgctatacc cctgtggtta tcgtatcatc atcatcggtt tgcgtcaaaa cctgattgag
      961 agctttgatt cgctccatcc gatccttaat tgttttcagc tgaatcacgt tttcacgatc
     1021 ctgctcttcg atccggctgt caatccaggc gatcgtttcc tcggttcgag gaatgacgat
     1081 cttttccaga gcgttgactt tccggattgt tttcttcact tcccgggcaa gacgcatgat
     1141 ggaaaccttc agctccgcca agtgtcccat caaggtaagg gcgctgcggt agcgatcgat
     1201 cgcgacctcg cttaagatgc tcgtctcttc gctggagaaa tagggacttt tatcgataaa
     1261 ggatgtttca acttttggca ccgaaactcc catcacccgc cgggatccca ccctgattga
     1321 ataatcgata ttgaccgccc cggccaaatt tccgaccctg agccggccca tctgcatgat
     1381 cgcgtcgctc agggcgttct gcgcttcttt gagggaactc agaacccggg tttcaaaatc
     1441 caccgcttgg tcgacaagcg tgagaagctc gacaaccaga atcgaccgct tctggtcgag
     1501 cagctcatgc ccgagcttgg cgaacttcaa atcttcgctg agcttgagca ggttggttct
     1561 tgtcggcgcc aaggtggtcg ttgccatatc agcctcggta gtactgggcg atctcttcgt
     1621 cggtgacgcg ctggagctct tcgcttggga gcttgctcag cgcctcccac ccgagatcga
     1681 gggtctcttc gatcgaacga tcctcatcat acccctggtt gatgaagcgg ttttcaaaga
     1741 aggtgccgaa ctccagatac tgctggtcga gggcggtgag ctcctcttca ccgattacgg
     1801 aggcgaggtt ctgcacatcc ttgacatggc tgtaggaagc gaagagctgg ttcgccaggt
     1861 gcggatggtc ctcccgggtc atcccctcgc cgattccgtc cttcatcagc cgggacagcg
     1921 acggaagcgg gttgatcgga ggatagagac cccgggcgtg cattccccgt tcaaagacga
     1981 tctggccttc ggtgatatat ccggtaaggt ccggcaccgg gtggctgata tcatcgttcg
     2041 gcatcgaaag aatcggcagc tgggtgatcg atccgctgcg gccgatgatc ttccccgacc
     2101 gctcatagat ctccgccagg tttgaataga ggtaccccgg ataccctttt cgggagggaa
     2161 tctccccccg tagcgttccg atttcccgaa gcgactcgca gtagttggtc atgtcggtca
     2221 tgatcacaag gacgtgcttg cccttgtcga acgccaaatg ctcggcaagg gtcagggcgg
     2281 tcttgggggt gatgatccgt tcgatcgacg ggtcatcggc gagcgagagg aagagcgcca
     2341 cattgtcaag gactccgctc tcttcgaagg aatcgatgaa gtacttggcg acatcgtact
     2401 tgacgcccat cgcaacgaag atcgtggcga aatcgcctcc gctgccccgc actttcgcct
     2461 ggcgggcgat ctgggcggca agctgattgt gggacaatcc gctgccggag aagatcggca
     2521 atttctggcc ttggatcagg gtggtcaatc cgtcgatgac ggaaatcccc gtctggatga
     2581 aatcccgggg atactcccgg gcggtcgggt tgatcggctc gccgttcaca tcccgttcgg
     2641 cgtatccttg aggggtcgcc gacccgtcgc ggctcttgcc cagaccgttc ataacccgcc
     2701 cgagcatctg ctcactgacc gccagagtca gcggctcgcc gaggaagcgc atccccgtgc
     2761 ccggcatcga caggccgtcg gtgccctcaa acacttgaac caccacgatc tcatcgctcg
     2821 tatcgaggac ttggccgagc cggatcttcc catccccggt agaaagctcg accagctcgc
     2881 catacccgac attgtgggtg ttccgcatcg tcaccagcgg tccgtcgatt cgggcggctc
     2941 cccgatattc gcggccggag aggaggtttg actgactgat attaatgttc atagattcct
     3001 cccaactcgt cgatcgcccg ctccatgcgc agttggattc ggtcgatctg gtgcagttca
     3061 tccgccttga tcgtaaagcg catccgcgcc atatcctgga ccgcccgcaa ccggcggagc
     3121 tttaccatcg gtactccgcc gttgatcgct tcactgccct tttcatagta cgcaaggatg
     3181 acgccgagca tcttcacctg cttcgccacc gggcagtaac ggtccacttc atcaaaagcg
     3241 ttctgctgca ggaacgcggt tttgaagagg gaacacacct ctaaaataaa gttctgggta
     3301 tccggcagag catccggtcc caccaatttc acaatctgtt gaagccggac ttcccgctga
     3361 agaagatcca tgatcttctg gcggtttcgg taccaggcgt cctcgctgtg ttcgctccac
     3421 cagtggcgca cgtcatcaag gtactcgctg taggaatcga gccagctgat cgccggatag
     3481 tgccgggaag aagccagacc ccgatcaagg ccccagaaac agcggacgaa gcgcctggta
     3541 tgctgggtga ccggctcgga gaagtctccg cccggcggac tgaccgctcc aataattgaa
     3601 accgacccct cccggtcgct gagggtcttc atataccccg cccgctcgta gaactgagcg
     3661 atccgggtcg gcaggtacgc ggggaatccc tcttcggcgg gcatctcctc catccggccg
     3721 gaaagctccc gaagcgcttc cgcccaccgg gatgtggagt ccgccatgat tgcaacatga
     3781 tagccctgat cccggtagta ctcggccaag gcgatccccg tataaatgga ggcttcacgg
     3841 gcgctgaccg gcatattgga ggtgttggca atgagaatcg tccgatccat caggctgttg
     3901 ccggttctcg ggtcgatcaa ctgggggaac tccctgagaa cgtccgtcat ctcgtttccc
     3961 cgctcgccgc agccgatgta gacgatgatg tccgcatcac accactgggc gattgagtgc
     4021 tgggtcatcg tcttgcccgt gccgaacccg ccgggaatcg cgaccgttcc ccctttcgcc
     4081 aagggaaaca gcgtgtcgat gaccctcaag ccggtgatca gggggcggtc gagggaaagc
     4141 cgctccttgg taggccttgg aacccggatt ggccatcgtt ggaccatcgg gacatccacg
     4201 acccggccgc cgggaagggt gattttcgca atcggttcaa caacggtgta cgaacctttt
     4261 ttgacgatct cggtaacgat cccctcttca atcgtcaaag gcaccatgat ccggtgttcg
     4321 atctggttgg tctcctgaac cgttccgaga atcatccccc tggaaacgga agcgcccttt
     4381 tttatcacgg gagtgaattc ccactgtttt atatgactga tcgcatcgaa ggagagaccc
     4441 cgggcgatga acgctccgga caagcccatc agctcctcca aaggacgttg aattccgtca
     4501 tagatctgcc cgatgagccc cgggcctact tcgacggata agctcatccc gctgccgtag
     4561 acgttatcat ccggcctcag gccggtggcg tcttcgtaga cctggacggt agcttcgcca
     4621 tcatggagct tgacgacctc accgacgagg cgctgctcgc cgatgtgcac cagctccatc
     4681 attctggcat cctgaattcc tttgacgatc agaacgggac cgttgacccg cttgatcttt
     4741 ccaataatgc gttcgttcat gcccgctcct gaatcattga tcgaatcagc cgctcaaagc
     4801 gtctgagccg gatgtcgact tggttgttga aggaggttga accatcgatc gaggagagga
     4861 caaccccaag tccctggata tacttctcct ccaggtgaag gcgaatcttc gctccggtgg
     4921 ctttgagaat cagggcttcc gctttcttca agtcttcctc ggtcacggga caaaggcggc
     4981 tgtaggcgac cttcgcttct ttccgatcaa gtccgatcgc cgcttcggcg atccaatacg
     5041 gcaaatgggg cttcaatgtc ttgcaatcga gggctacaaa caccgcatcc atcacccgtt
     5101 gataggaatc atcaacctgt ctcaacgccg ctttccgttt ggctgacgct ttgttggtcg
     5161 caaggcgcaa ctggatctgc ttcacttttt catcggtggc gcgcttttgg gaagcgacct
     5221 cctcttcggc gcggctttgc gctcgttcca gaatcttttt ggcttgtgtc cgggcatcct
     5281 caagatggtc ttgcgcactt cgggccgctt ccttgagaat tccttccagc aatggcgttt
     5341 ctgtactctt catctcttca tcctcacaac gacactccga tcgcctgatt cacgagggct
     5401 cggaggtcac tgcgtttcgt gccgctcttc ggcgagggaa tctccacgac gagggggaac
     5461 gtttcgctga acaggtatcg atcaacggag gatcgaatca tgttggcgac ttcatcggtg
     5521 atgatgatga ttccgttctc tttgtcctcg agcgcttttt cccacgcgat tcgggcttct
     5581 tccgggtttg ttgcctgcat gccgaataca ccgaccaggg agagacccaa gacggtgtct
     5641 tcgtcaccga tgacaacgta tctcatcgtc aaaccttcgc gagaatcatc agactggtga
     5701 tgaagccgaa aacgacgact ccttcggcga gagcgatgaa gataagcgct tgagttccga
     5761 tctcgggtcg ttcactcatt gcacccatcg cggcgcttcc gacccgtccg atcgccattc
     5821 cggcggagat cgcaccgacg gagaacgcca gcgccgcggc aaacgccacg agggcaaggt
     5881 tgtaatccaa tccggttgtg gctaccgctt cggtcgtggc ggcgtaggcg ctcggggcga
     5941 agatgaaccc tgaacccacc agcaccaggg cggttgcaat taagctcatc gtgacttttc
     6001 ttctgaacat cgatttggtc atacgagacc ctcctatcaa atgcactaaa ttatcgatcg
     6061 gcggacgaag accgcttcag tccaactggt ctgtaggcga ttcctcgacc ggtaaaatac
     6121 cttgagaaga attcgtagta gttcaacctg agcgattgaa tacctgcact gaggccctcg
     6181 aggacgatta ccaagaggtt gcccgcgatg aagatgagaa cacccgccac tccgccgacc
     6241 atcgactgga gatacttgaa cgttcccatc aacgaagcgt gggcgatgcc aagaccggca
     6301 acccgcatga aggagagggt gttggagaga tacccggtga aaatctccag aacatccacc
     6361 aggaactcca tgatcgagcc ggcggccacc tggccgaacg tcttgtccgc cctcccttgc
     6421 ttaaggtcga cgaagtgttg cacatacggc ttggcgaaga gcagaatcag gcagatgacg
     6481 agcgtgggaa tgatccaggg atcggagggg aaggagcgat agctcgatcc gacgaacccg
     6541 aacgccatat agattccggt cccgaacagg acaccgccga cgaccccgtt cttgtcaagc
     6601 agcatcggaa cgaaggattt cttcctgaac aggttgatcc agttcaaaac gaggccggtg
     6661 tagatgatga tgattccgaa cctgatcgtg atgccgagaa tcccgtacac gtcgcggacc
     6721 agtgcagtgg cgggagcatg cccttcgact gccgcctcga agttgaacca cagcggcggc
     6781 aggagcggca ggccgaagta ggatccgaac agcgctccga agagcatcga ggagagcccg
     6841 aggtagacga gcaggttcgt gacattgcgc gtcaggagcc cttccttctt ctccggattg
     6901 cggcggtagc tccgcgatcc gaagagtccg accaacagca ggacgagccc ctgccctaca
     6961 tcgccgaaca tcaggccgaa catcgaaagg tacgcgatca tcacgaagat cgtcgggttg
     7021 acggtgccgt actccggcgt ggcgtagttg ttgaccatgt tctggaacgg ttccagcact
     7081 ttggggctgg ttaccgcgac cggaacgctt tcacggggaa cctccgtcgc ttccgtccac
     7141 tccacgacgc actgcccgcc gcttgcttcg acgattgcgt ttcgaacgct ctcagcttga
     7201 tcggcgggaa cccatccgct gaagagggtc gtattgcggg tataggagaa atagctctta
     7261 atctggtcgc tgagctcgtt gagccggaga ttcgaccaga tcgcatggag ctcttctttg
     7321 cgttcggcga tcgctcgttt gaccgattgc tcaatctcgc cccgtttatc aatgagcttt
     7381 tggtgctccg tttcaagacg ggttcggaca agcacgaccg cctgcttctg aagcagcggg
     7441 tcgctttcct ccgtccagcc gaacttctcc aaaagcggat cgatttgcgc aagatcccgc
     7501 cgaagggaaa gcacggttac aaactcctcg tcgatcggct cgacgaatgc accgtacggc
     7561 gagagcttct cctccagctg ttcgctcttt ccctccttca cgcttccgac tcgcagatcc
     7621 agatattgcc acttggattc agcgaggtat cggcgcatct cctccacccc caacagggct
     7681 tggttggcga ctttttggcg ctctttaagc gcaagcaggg tttgagtcag ttcatcgagg
     7741 accgcccgat acgcatcgac ctggggttcc gccaacaggg atacgtccaa cgtgctttcc
     7801 ccgggaaggg caaggtgtcc ctgtttcagg agcgcttcaa ctcgacctcg aaggtcttcg
     7861 agcaccaccc ggttcgtcgt actcggtttg gaggagagct tcgccagctg gtctgcttca
     7921 agccggctga tgtgaatgaa atccaacaca cccagctgga gcaacgcttt gaccacctgg
     7981 tcgcttcgtt gctgtagtac taccgcagta agcagtcgca tcggctttgt gaataagttc
     8041 atccgattac ccccctgata tatccttcat caaatccata atactttcca ttcaaaatcg
     8101 ccttgatccg cctgaattcc tctttgttga ggaagaaata cgccaaggcc acaccgatgg
     8161 tgaacgggtc tcccccgagg aggcgccgat acactctctt ttggttttga gccagaagct
     8221 cttcgattcg cagcacctct tcaaccgccg agtgatcacc gacccgttgc tcatcgggca
     8281 ggaactgcga aagcagttcg cttgccgagc gattctcctt cggcgtctcg atatagcggt
     8341 tcacctcttt gctcttgaaa acctcccccc acggcaggag caaggagagc agctcctttg
     8401 cttccagacg gtgataccat ccgtagcgaa caaggatgag gaggttcttc agatcgatct
     8461 cctgatcaag aagttccaac gctttttgac gatcggtttt cttcagccaa gcggtcgcct
     8521 tgcgaatctc ttcataccac acccggtcga gccggctctc caattgaaac agcccttctt
     8581 cccgcaaaga ctcttccgta tactccggaa gaatcggatg gtagggggtg tttgccgtgg
     8641 cctctaccac cccatcccat gagagcgcat tgatgagcgc agtccaatcg accggatgaa
     8701 gaatcacctc cttgaagagg tattcactcc gatgacgaat cggacgacgg tggatgatcg
     8761 acgaatacca gagtcgaatc gaattcttca ggttgtcgat ctcgattttc ccaagcagtt
     8821 ccgctacgaa ccgtccctcc actccatcca gatattgggc gatctcccga tacagggcga
     8881 tctccatctt gagcagaacc agctcgatgg attggatatc cccgctttga tcatacgaac
     8941 gggccaaggc ggcatatcgg gtcggtctta aggcggcaat cgcatccacc agggtcggag
     9001 cacgcaaaag agcctcggta accgcttctt cccgcgcttt accgagtcgg gcacgcatct
     9061 tcgcattgac aaatccgtac gtatctactg agctcattat gactcgctta ttccatccaa
     9121 attggtttta cacacctcga tgacgatcgc ttgggcgatc tctttcactt cttcggcggt
     9181 aagtcgggaa gagcggtcca atgaggcttc atattcggtc aattggtcga ccaagtccgc
     9241 ttcggcttga gcaatcgttt cttgggccag gattcgatct tgtttcagcg catcccggac
     9301 aaacgcattc gcttccgtct gggcattggt gatgatttca cgagcttccc gctccgcatc
     9361 ccggactatt tgctgagccc gatcttcaac cgttaatact tcgttgataa tatcactgcg
     9421 cacgtgccga cctcccaccc ttacaactcg atatgctttc tctcatatgt tcttcttttt
     9481 gctcaaaggt gtcaagcaaa cattagggcg cagcggtggt gactgtttgt tcggtgggag
     9541 gtgattccca atcaccgatc atctcgtcaa agaccagcgg atcgaagtag aagtcatcgt
     9601 ccgtcacgaa ggcatcgtcg aagctgaaga acgggtcctc atcgaatgac gggaagatcg
     9661 cattccagaa gctctcctca tccgtttgct cgattacgtt gagggtcaga cgggcatcat
     9721 actcatccgc cacttcgaca tggaaggcat cttcaatctg agcctcaaga agtacggcga
     9781 ccttatcgaa atagctgact cgaccttcaa aggcggggtc gtccacctga taggactcaa
     9841 gaagcaatat ggtgttgatt cccatcgtct tggcatcctg ttcgggcaca tcgaagcgga
     9901 ctccatacca atcgtcatcc accttcaaat caaagaggta ggatccggct ttcacctcat
     9961 tgaggatgaa ccgtccatcc tggtcggtga agaggtagag cgtatcgtcc cgatcatgtt
    10021 tctcctcgcc ctcctcgttg gtcgtgatcc gatataccgg cgatgagtat tggatgtacg
    10081 gggttccatc cttttggatg agcactccgc tgacggtaaa tgccggctgc atgctgattt
    10141 tcacgacgaa gctctctctc ccccgcggcg aaagcgaata cacgaaagag cttccggtgg
    10201 agaattcggt gggaccgctg gagaaaacgg cgacggtgtt ggtcgtattg gtggaaagcc
    10261 cattgtacaa tgcgcttccg aagggacggg gcagataggt gggactgctg tcgaggcttc
    10321 gggcgaccga gacctggcct cgcttgagga gccccgtcgg cttgaccagg aggaaggaat
    10381 cactgacgct gcgcccaagg ccgaacgctc caccggcata cgccaacgcg gtgttgatca
    10441 taaccgtggt ggtcatattc ttctgagcaa gcgaatactg gtgcctcaga ttcaaggaag
    10501 agaacgtccc cgaatgattc caggacccgg cgagaactcc cgccccgaac ttcgggggac
    10561 tgccatcgaa ttccgtacga actccgctgt aggaaagagt gatgctgtca tcctttgaag
    10621 gacgcagact catgctcagg ttcgaattca ccccggtcgc ttgaattgaa ttcgtattgc
    10681 tcatgcttgc tttggagccg atggagtagc ttccgctgat ttgaccggtg agaatcggtt
    10741 tggaagtgtt gctcgaaccg gttaacgaga ttgaaccgtt tatcgaaaac cctttaaagg
    10801 gcgagaagcc ggttgaggcg ctgaccgacc atgtagcgag ggacgtatcg aaggtatagg
    10861 cgccgcttcc gctgagcgag tagcggagca ggttgaacag gctgtcggag tatgagaggg
    10921 agacggaggc gcttcctttc gagtcgaccg gtcggttgta gttgccggac aacccgagcc
    10981 ggttcaaaaa agacgtttca ctgaggccga agcgttggcc gaggttcatg ttatatgagg
    11041 ctttccatgt atcggtctcc acatgtgcag acccctggat ctgggtcgat ccgatcattg
    11101 tggcaaagac gatccccaac gatgcctgga gggttccggg ggtgaatgca aggtcgatac
    11161 tcgtcgtcaa cgcatcggtg acacccagtt cctgccggta tgtcgcggta aacgctttcg
    11221 ggtggtagga aagataatcc cccccaagcc agggaagagc gaacgagccc tccttatttg
    11281 gaccgcggct ctgaggcatg ctgagtccga tggcataggt ggtatcgcct ttggccaaca
    11341 ggcggtaatc gtatccgaaa ttcaaataga tcggtttttc cggtttgccg gtggaagtcg
    11401 gtgtcacctc aatccggatt cggtttgccc cttgaacgag aatgaaatcc cgcaggcggt
    11461 agacgccggg cttgagtctc cgagtataaa aggacttgtc gttcaggaag atctcaacgt
    11521 ccgattcttc gacaatggta agagtttctt caatctgagt acgctttgca cccgttgtac
    11581 cgaaagcgta gtttttttcc agacttatac cgatactgga ctgggctccg aggattttac
    11641 ccaaaccgct gccgatgttt ccaaaggaaa gccggatgct ggcatcgacg aaatcgtaat
    11701 atccgttcca tgagccggga ttatagtagg gttcctgatg ggaaagggtg aagttgtagt
    11761 tcagcgccac cccgaagagg gaagcgcgat tggaaacgga aaggttcagc agcgtccggt
    11821 tgaatttact gaaatcatcc ggatattcca actgaccgaa caggttcaaa ttcgccgcca
    11881 agctgaacca ccgcggcttc aagggaattg ctccactgat cgcgtactgt tcgcggcggt
    11941 tgaccgtggc ggatgagaga ctgagaattc gttcgggcat gtcttggatt gagaacgtca
    12001 gatacagaac gaaagcctgt tcatcataca ccgtctcaac tccacgtctt tccaactcct
    12061 caggagtcac atgggagcca agatcggaga aaattcgaat acgggccgcc attgtaagat
    12121 gttcggcgac gtagaactgc agatcctcca cgttgaccag gcgttcctcc ccttcgaaaa
    12181 caacttcgat tgttcctaca agatcctcgt tgacaaagag gcggaagtaa taggtgccat
    12241 catcatacgc aacggcgcta tcctcgccga cgacaaagaa gtccgcccaa aaatcatcat
    12301 cggcggg
//
LOCUS       DAOSLI010000048        12295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_5179_length_12295_cov_17.379085, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000048 DAOSLI010000000
VERSION     DAOSLI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5179_length_12295_cov_17.379085"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..834
                     /gene="larE"
                     /locus_tag="PLF67_07175"
     CDS             88..834
                     /gene="larE"
                     /locus_tag="PLF67_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOE89825.1"
                     /translation="MDLNEFFAQNDRVAIAFSGGVDSSYLLYAAGKAGVSSKGYFVKS
                     EFQPASEVEDAKKIAAYAGVEMEIIRCSILDAPKIVENGESRCYFCKKKIFSHIQEKA
                     KADGFTIILDGTNASDDISDRPGYRVLQEMKVLSPLRICGLTKEQIRKRSKEAGLTTW
                     DKPSYSCLATRIPTDTAIKQSMLEATEHAEEYLKSLGFTDFRIRYLSGYAQIQLKNSQ
                     LTAFLAQKANIKNKLETWYSGVLLDMETRE"
     gene            831..1580
                     /gene="larB"
                     /locus_tag="PLF67_07180"
     CDS             831..1580
                     /gene="larB"
                     /locus_tag="PLF67_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOE89826.1"
                     /translation="MNDYKKLLEGVKEGSISIEDALLEIKKKPFEDIGFAKIDHHRSL
                     RQGAGEVIYGAGKTPEQIAEISKRLLERSERILITRLDDKQAKDLSECLEFTYFPGTG
                     IGMVGSLPEPTTQGIIVVATGGTSDIPVAEEAALTAEFYGNRVTRLYDVGVAGLHRTL
                     SHLDELMSASVVIAIAGMEGALASVLGGLCDSPVIAVPTSVGYGASFGGLSALLSMLN
                     SCASGVSVVNIDNGFGAGYLASMINKKGENR"
     gene            1577..2794
                     /gene="larC"
                     /locus_tag="PLF67_07185"
     CDS             1577..2794
                     /gene="larC"
                     /locus_tag="PLF67_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOE89827.1"
                     /translation="MTIYIDAGMGAAGDMLTAALVEVLGNKEKTVRELNHLGIPGISF
                     HSEKSEKLGIVGTRMVVRYNGKEEGIDGEVIHHGHGHEHDHPDHGSDHHHHGHSSLHD
                     IEHIVSSHIHVDEAVKKDIMAVYRMIAEAESHVHGKTVDEIHFHEVGTMDAIADITAV
                     CYLFSKLNPDKVVVSPIHVGSGTVTCAHGIMPVPAPATAYLLQGCPIYSSEIKGELCT
                     PTGAALLKYFATEFGEIPIMKVSSIGYGMGKKDFERPNAVRIMAGEEEGKKDVITALE
                     CNVDDMTSEELGFAMERLMEEGALEIFATAVVMKKSRPGFLLSVLTDEKTKEKMISLI
                     FSLTSTIGIRETRHSRYVLERKTEEVETRYGIVKKKISHGYGIKKEKFEYDDLATIAR
                     KEHLSIREVRESL"
     gene            complement(2778..3554)
                     /locus_tag="PLF67_07190"
     CDS             complement(2778..3554)
                     /locus_tag="PLF67_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE89828.1"
                     /translation="MEKPFTDIHATAESQLDGKPLTIEDTRHIADARVLPETVENLIL
                     LGKALSKSLRYLEAIEAYSKALALNPDSPEARRLRAGRYLSTLQTERAIEDFRRALET
                     GGDALDCRYRMGLAFYFQGDYEKAMEEFSSVLFLADPEMEIALIYWHSLASLKAKKDP
                     SFLHREVAEEAVGHHYSYREGVSFLKGELTEDGLRKRAEAESCDMEYSMKMYALHVWK
                     ESRGKKDTLLKDILKRDEYWFCFSSLAAWQERRRALRGSP"
     gene            complement(3554..4084)
                     /locus_tag="PLF67_07195"
     CDS             complement(3554..4084)
                     /locus_tag="PLF67_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOE89829.1"
                     /translation="MQNEYLELWNTCVSFHGHGCGGLAVGFRASLYALELLEAERSVD
                     EEIVCIAEGDACGVDAIQVLLGCTVGKGNLIFDMQGKNAFSFYNRKNGKSVRLVLRRT
                     PDKDRDQRLDWLMNGDYHEMFDVKPAPENVPEKSRIFKTYLCETCQEGVSENYVRVKE
                     GKMVCSRCAHTYTRGL"
     gene            complement(4093..4899)
                     /locus_tag="PLF67_07200"
     CDS             complement(4093..4899)
                     /locus_tag="PLF67_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89830.1"
                     /translation="MKRRNLPLYLGLAILLLFVLVALVPSLFTSYSRKDMFPPWQGMS
                     SEHFLGTNNLGYDIYTELIYGTGDTLFIGVTSSILSLVLGLAIGTLSSSESFVGSIFT
                     SLTNMFVYLPKLVTLIVLSTFLGSSSFTLIALISAFGWVGTSRVVKGKIVHLSSAPFI
                     ENCTLLGYGKLHTVLHHYVRNLQDVIVSRFLAGATSCIMMESTLSFLGFGDLYHPTWG
                     TMVNYAWKRGAMLRHAYNYLLAPGFAIMALSLSFYFLSLFFEKKQEEINL"
     gene            complement(4896..5876)
                     /locus_tag="PLF67_07205"
     CDS             complement(4896..5876)
                     /locus_tag="PLF67_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89831.1"
                     /translation="MTKYRKDIVTALFAFCFVIILNFILPRILPGDPIAYLTGFAEED
                     MTVKQYEYYEDALHLKAPLPEQFLHYLKSLGDGSLGYSYKKEATVRSLIFNRLGHTLQ
                     ITLTSVCITSIIALVLGLSAGLSKGGSFDRAVTTSSVILNTVPTFMIGIVLLLFFAFH
                     KKWFPYANLSSTGVRKGTVSFFLDRIWHLFLPVLTLVLAQLPSRFLLVRNLAANISEE
                     KYVLYAKTRGLSHRTIKYHYIFPNIAAPFINLVGMSVGSALGGALVIENLFSINGMGT
                     LLTDAVYSLDYPLIQGILFVTTAFMTLSIILSDGLCLVVNPKLRKKERTQ"
     gene            complement(5890..7527)
                     /locus_tag="PLF67_07210"
     CDS             complement(5890..7527)
                     /locus_tag="PLF67_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89832.1"
                     /translation="MKHRLSVLVIILLSSALMLFARPVKESPAGESSSFAVERITVGT
                     TAKIEKAERGEYAFDMLASAVTELPLVYQDTSGSYHPLLVDFATEDGVSWTFTLKDGL
                     KWSDGVAVTAEDILFTFQHEDASGSAYLTDQTDSKGKVTKAKYVSCTVSADNRSITLV
                     LPSANVRMLGDMTSFRILPKHIFENNASPTVADKRIGCGPYTFDSFSAESGTIVFKAN
                     ADYPETPRVGEIVYRIFGNEDTMYLALQNGDIDIVWNYSMGVPATYQDFLASSPAVSL
                     VAVPATNAPAVLAFNNAKGPFADENLRKAVSYALDYDLFKAYFGSEYASVPNRGFVPP
                     TTVGYKDTEKLGHDIGKADGFMAAAGYRKNALGVYVDSDGEPMSFALTVNSSKQTHLG
                     YAELIKTQLEAFGMQVAIDGLDGTSYNAKTSNRFSNNNITTEAAIYGYTSAGMGMMNG
                     LGSIYVDGTHAVQGGCQVFDPEFQAVIARLASSRNLEEYYVAAGALQDFYAAHTPLIA
                     LYWDNLIYAYSSGLGNLTVDAVFGLNNVNNWMTISSR"
     gene            complement(7712..8392)
                     /locus_tag="PLF67_07215"
     CDS             complement(7712..8392)
                     /locus_tag="PLF67_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOE89833.1"
                     /translation="MVVQRMIEVKSVSKAFGKKNVLSDLSFSLDTGEILGVYGKSGIG
                     KTTLAKILCGLVLMDEGEILIDGTMLFSPTSPYDRKLGRGIQMVYQHPYASLDPNQKI
                     RGSFSELIRYHGLAKGKEETETLILRVLSEVNLDEGILSHLPRQISGGEAQRVALARC
                     LLLQPTLLIMDEATSMLDVMVQANVFALVKKNVIEKGGSILLISHDRDLVDHVSDRIL
                     DLEKNRVG"
     gene            complement(8374..9309)
                     /locus_tag="PLF67_07220"
     CDS             complement(8374..9309)
                     /locus_tag="PLF67_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89834.1"
                     /translation="MATSKRKGREEGEKSLLRYSSGTIYAKTLAGNRTLVQDVSFSIR
                     KGEKLSLIGETGSGKTMIALSLMGKLAPNVKTEGLQCILDGKDCSHPKVASSLLGREV
                     AYIPQNGMEFLNPSRTVKHHLYDSLHRAGVRSAEAKDKAASALSSVGFRDIDEILKSY
                     PFQLSGGMAQRVTVALALCTRAKLIIADEPTNGLDKEAASRMLEHLGLLFPDAGQLFI
                     THDIGVASNADRILVLCGGRTMEYGRNFFLQNPRNAYTKRLVSSLVSNGMKATPTLRK
                     RTGCPFSDRCPEYSERCRDERIHQREGEHEWWCNG"
     gene            complement(9456..10511)
                     /locus_tag="PLF67_07225"
     CDS             complement(9456..10511)
                     /locus_tag="PLF67_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE89835.1"
                     /translation="MRTFDYYGLREASWDAEILSFVAQISVYKGKQDLYLREKPAELR
                     RLVEVAKIQSTDSSNRIEGIGTSNARMRQLVEKRTAPRNRDEQEIAGYRDVLSTIHES
                     YEHIPLTSDVLLQLHRDLLAYTDMTFGGKFKNTRNHISEIHADGTSSIRFTPLEPFMT
                     PMAVDAICEQYRRALEEHMVNPLILIPIFICDFLCIHPFNDGNGRMSRLLTTLLLYQS
                     GFMVGKYISIEQKIERTKSTYYDVLGYASVGWHEEKNDYTSFIKYFLGIILSSYKDLE
                     ERLGAVDRKNTPYEIVRKAVDNTLGVFTKTEILHRCPSIGSSSVEAALKRLKDEGYIL
                     RQGRGRNTAYVRNPNHR"
     gene            complement(10567..10767)
                     /locus_tag="PLF67_07230"
     CDS             complement(10567..10767)
                     /locus_tag="PLF67_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89836.1"
                     /translation="MRIFDHCKFRERSWVQGALFRRKISECKGRQDLYIGALNARLKE
                     LEEDILRRLGNVPKNCVISHRD"
     gene            10959..12080
                     /locus_tag="PLF67_07235"
     CDS             10959..12080
                     /locus_tag="PLF67_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89837.1"
                     /translation="MIIVKPVVTRKEWNAFFAFPNDLYKGNKYFVPYLIADEKDTFSP
                     KKNPAHEYCDTQLFLAYKDGKVVGRIAGLINNKLNAMKGMKQMRFTRWDVIDDIEVSK
                     ALFEAVFAWGKAKGMTEIIGPIGFSDLDKQGLLVEGFDQVGMFITLYNHPYYHEHLTK
                     LGFVKDVDWIEYTVYTPKELIPRVKRISEMAQERHGYRLLEFTKKKEIIPYVQEIFHM
                     YNESYAHLYGFCPLTDGQIEMAVDQFFMVAQLDYIYVVVDKEDAVIGFGLLIPSLSKA
                     MQKSNGRLFPTGLFRILRSLKRYDVLDMYLIAVKPEYFGRGVNAVILHEGIKKAIEHN
                     VVYAKTGPMLETNTYIRTQWRIFETEQNKRRRCFKQVFK"
BASE COUNT         3156 a         3137 c         3148 g         2854 t
ORIGIN      
        1 gacatgatca ggtactacct atgccctgaa aagtatttcg gctatcattg gagatgaaga
       61 cggattcaga gggggatcgg ataagagatg gacttgaatg agttctttgc acaaaacgac
      121 cgcgttgcga tcgctttttc cgggggagtc gattcttctt atctcctcta tgcggcaggc
      181 aaagcgggag tgagctccaa aggatatttt gtgaagagcg aatttcaacc tgcatctgaa
      241 gtcgaagatg caaaaaagat tgcagcctat gcaggggtgg agatggagat catccgatgc
      301 tccatcctcg atgcacccaa aatcgtcgag aatggcgaga gccgctgcta tttctgtaaa
      361 aaaaagattt tttcccatat tcaggaaaag gcgaaggccg atgggttcac catcatcctc
      421 gacggcacga acgcctctga cgacatttca gacagacccg gctaccgggt tcttcaggaa
      481 atgaaggtcc tctcccctct ccgcatctgc ggtcttacaa aagagcagat cagaaagcgt
      541 tccaaagaag ccggattgac cacctgggac aagccgagct attcctgtct cgccacccgt
      601 attcctacgg ataccgcaat taaacaatca atgctggaag caaccgaaca tgcggaagaa
      661 tatctgaaaa gtctcggctt cacggacttc agaatcagat atctctcagg ctacgcccaa
      721 atacaactaa agaacagtca gctcacagct ttcttggcac agaaggcgaa cataaaaaat
      781 aagcttgaaa catggtattc aggagttctt ctggacatgg agacaaggga atgaacgatt
      841 ataaaaaact cctggaagga gtaaaggaag gcagcatctc catagaggat gcactgctcg
      901 aaatcaagaa aaaaccattc gaagacatcg gttttgccaa gatcgaccat caccggtctc
      961 ttcgtcaagg tgcaggtgag gtcatctatg gagccggaaa gacacccgag cagatcgctg
     1021 aaatatccaa acgtcttctg gaaagaagcg aaaggattct cattaccaga ctggatgata
     1081 aacaggcaaa agacctctcc gaatgtttgg aattcaccta tttccccgga accggaatcg
     1141 gcatggtcgg gtctcttccc gagccgacga cacaaggcat catcgtcgta gcgacaggtg
     1201 gaaccagcga cattccggta gcggaagaag cggcgttgac tgcggaattc tatggaaatc
     1261 gcgttacaag actctatgat gtaggcgtag ccggtctaca caggacgctg agccatctcg
     1321 atgagttgat gagcgcttcc gtcgtcatcg ccatcgccgg aatggaagga gccctggctt
     1381 ccgttctcgg agggctttgt gacagcccgg ttatcgccgt tccgaccagt gtggggtatg
     1441 gggcctcctt cgggggactt tcagcgttgc tctccatgct gaattcctgt gcaagcggag
     1501 tatccgtcgt gaacatcgac aacggcttcg gggccggcta tcttgcctcg atgatcaaca
     1561 agaaaggaga aaaccgatga caatctacat cgacgccggc atgggcgccg ccggagacat
     1621 gctgacagca gcccttgtgg aagtgcttgg caacaaggag aaaacagtcc gggagctcaa
     1681 ccatctggga attcccggta tttcctttca cagtgaaaag agcgaaaagc tcggcatcgt
     1741 tggaacacgg atggttgtcc gctacaacgg aaaggaagaa ggaatcgatg gtgaagtaat
     1801 ccatcatggg cacggacatg agcatgatca tccggaccac ggttccgatc accatcacca
     1861 tggtcactcc tccctccacg acatcgagca catcgtctct tcacatatcc atgtggatga
     1921 agctgtcaaa aaagacatca tggcggtcta tcgcatgatt gcggaggcgg aatcccatgt
     1981 tcatgggaag acggtggatg agatccattt ccatgaagtg ggaacgatgg acgccatcgc
     2041 ggatatcacg gcagtctgct atctcttctc caagctcaat ccggataaag tcgtggtctc
     2101 gcctattcat gtcggttcag gcaccgtcac atgcgcccat ggcatcatgc cggttccggc
     2161 accggcgaca gcctatctgc tgcaaggctg cccgatctac tcttcagaga tcaagggaga
     2221 actttgtacc cctacgggag cggcgcttct caaatacttc gccacggagt tcggagagat
     2281 accgatcatg aaggtctctt ccatcgggta tggcatggga aagaaagatt ttgaaagacc
     2341 caacgccgtc agaatcatgg caggagaaga agaaggaaaa aaagacgtca tcacggcgct
     2401 ggaatgcaat gtcgatgaca tgacctccga agagctggga ttcgccatgg agcggcttat
     2461 ggaagaaggg gctctggaga ttttcgccac tgcggtagtc atgaagaaat cccgtccggg
     2521 cttccttctc tccgttctga ccgatgagaa aacgaaggaa aagatgattt ccctgatctt
     2581 ctccctcacc tccacgatcg gcatcaggga aacccgccac agccgttatg tattggaacg
     2641 caagaccgag gaagtggaaa cccgctatgg aatcgtcaag aagaagattt cccatgggta
     2701 tgggatcaag aaagaaaaat tcgagtatga cgatctggcg accatagcaa gaaaggaaca
     2761 cctttccatc agggaagtca gggagagcct ctgagcgctc ttcgacgttc ctgccaagcc
     2821 gccagagaag agaaacagaa ccagtattca tccctcttga gaatgtcttt caggagggtg
     2881 tcttttttac cccgcgactc cttccagaca tgaagcgcgt acatcttcat ggagtattcc
     2941 atatcgcatg actcggcttc agcgcgtttt ctgagaccat cttccgtcag ctcccccttg
     3001 aggaaggaaa caccctcccg gtaggaatag tggtggccaa ccgcttcttc ggctacttca
     3061 cgatgcagga atgacggatc cttcttcgcc ttcaaggatg ccagactatg ccaatagatg
     3121 agcgcgattt ccatctcggg atctgcaaga aacaacacgg aagagaactc ttccatcgcc
     3181 ttttcgtagt ctccctgaaa gtaaaaagcc agccccatcc gatagcggca atccagagca
     3241 tctcctccgg tttccaaagc cctccgaaag tcttcgattg cacgctcggt ctgcaaggtg
     3301 gaaagatatc gtccggcacg caggcggcga gcttccggac tgtccggatt caaagccaaa
     3361 gccttggagt aagcttcaat ggcttccaga tagcgaagac tctttgatag agccttgccc
     3421 agcagaatca gattttcaac cgtttcggga agaacccttg catcggcgat gtgccgggta
     3481 tcttcaatgg tcaacggctt gccgtcaagc tggctttccg ccgtggcatg gatgtcggta
     3541 aaaggttttt ccatcagagt cctctggtgt aggtatgggc acagcgggaa cagaccatct
     3601 tgccttcctt cactcgaaca tagttctcgg agaccccttc ctgacaggtt tcacagagat
     3661 aggtcttgaa gatcctggac ttttccggaa cgttttcggg tgccggcttg acatcgaaca
     3721 tctcgtgata gtctccattc atcagccaat cgagtctttg atccctatcc ttgtcgggtg
     3781 ttcttctcaa gacgagtctg acggactttc cattcttcct gttgtagaag gaaaaggcgt
     3841 tctttccctg catgtcgaag atcagatttc cctttcccac ggtgcatccg aggagaacct
     3901 ggatggcatc caccccgcag gcatcccctt cggcgatgca gacgatttct tcatcgacgc
     3961 tgcgttccgc ttccaacagt tccaaggcat agagagaagc cctgaaaccg acggcgagac
     4021 caccgcagcc atggccatga aaagagacgc atgtgttcca aagctcaagg tattcgttct
     4081 gcatggatac tcctatagat tgatttcctc ctgtttcttc tcgaagaaga ggctgaggaa
     4141 gtagaacgac aaggacaacg ccatgatggc gaagccaggt gccagaagat agttgtacgc
     4201 atggcgaagc atggcgcccc gtttccaagc atagttcacc atggttcccc aggtgggatg
     4261 atacaggtcg ccgaaaccaa ggaagctcaa agtggattcc atcatgatgc aggaggtggc
     4321 gccggcgagg aatctggaga cgatgacatc ctggagattt ctgacatagt ggtgtagaac
     4381 cgtatgaagc ttaccgtagc cgagaagcgt acagttttca atgaacggag cggaggaaag
     4441 gtggacgatc tttcccttca ccaccctgct tgttcccacc cagccgaagg ctgagatcag
     4501 ggcgatcaag gtgaaggagg aactgccgag gaaggtggaa aggacgatca gggtgaccag
     4561 cttcggaaga tagacgaaca tattcgtcaa ggatgtgaag atgctcccga cgaagctttc
     4621 cgaagaagaa agcgttccga tggctaaacc caggaccaaa gacaggatgc tgctggtgac
     4681 cccgatgaag agagtgtctc cggttccgta tatcaattcc gtatagatgt catagccaag
     4741 attgttggtg ccgagaaaat gctcggaaga catgccctgc cacggcggga acatgtcttt
     4801 tctgctgtag gatgtaaaga gagacggcac gagcgcgacc aggacaaaga gaagaaggat
     4861 cgcaaggcca aggtagagag gaagattgcg cctcttcact gtgttctctc cttttttctg
     4921 agttttggat tcaccaccag acaaagacca tcggaaagga tgatggaaag agtcatgaac
     4981 gccgtggtga caaagagaat tccctgaatc aacggatagt cgagggaata aaccgcatca
     5041 gtcaaaagag ttcccatgcc attgatcgag aagagatttt caatgacgag agcccctcca
     5101 agggcggaac ccacgctcat gcccaccaga ttgatgaagg gggcggcgat attcggaaaa
     5161 atgtaatgat acttgatggt tcgatgggag agacctctgg tcttcgcata cagaacatac
     5221 ttttcttccg aaatattggc agcgaggttg cggaccagaa gaaatcggga aggaagttga
     5281 gccagaacaa gagtaagcac aggaagaaac agatgccaga tccgatcaag gaaaaagcta
     5341 acggttccct tccgtactcc tgtggaactg agatttgcat agggaaacca cttcttgtga
     5401 aaggcgaaaa acaacagcag gacaataccg atcatgaagg tcggcacggt gttcaagatg
     5461 acggaagacg tggtgaccgc ccgatcgaac gaaccgccct tcgacagccc ggccgacaat
     5521 ccaagcacca gagcgatgat ggaggtaata catacgcttg tcagagtaat ctggagagta
     5581 tgccccaaac gattgaaaat gaggcttcgg accgtggcct cctttttgta ggaatatccc
     5641 agactgccat cacccaaaga tttcagataa tgaaggaatt gctcgggaag gggggccttt
     5701 agatgtaacg catcctcgta atactcatat tgtttgacgg tcatatcctc ttccgcgaat
     5761 cctgtcagat aggcgatggg atctccggga aggatcctcg gaagaatgaa attaagaatg
     5821 atgacgaagc agaaggcaaa caatgcggtc actatatcct tgcgatactt cgtcatcatt
     5881 ctttccctgt catctggatg aaatagtcat ccagttgttc acgttgttca atccgaaaac
     5941 ggcatcaacg gtcagattgc cgagtccgga agaatacgca tagatcagat tatcccaata
     6001 gagggcgatc aagggggtat gagctgcata aaagtcctga agagcacccg ctgcgacata
     6061 gtattcttcc aggtttctgg aagaagcgag acgggcgatg acggcctgga attccggatc
     6121 gaagacctgg caaccaccct gcacggcatg ggttccatcg acgtagatgg atcccagacc
     6181 gttcatcata cccataccgg cggaagtgta gccatagatg gcggcttcgg tggtgatgtt
     6241 gttgttggag aacctgttgc tcgtcttggc gttgtaggaa gtcccatcca gaccgtcgat
     6301 cgctacctgc atgccgaacg cctccagctg ggtcttgatc agttcggcgt agccgagatg
     6361 ggtctgcttg gaggagttga ccgttagagc gaaggacatc ggctcgccgt cggaatcgac
     6421 atagacgcca agagcgttct tccgataacc cgccgccgcc atgaagccgt cagccttccc
     6481 gatgtcatga ccaagctttt ccgtatcctt atagcctacg gtcgtaggcg gcacgaaccc
     6541 tctgttcgga acgcttgcat attcactgcc gaaatacgcc ttgaagagat catagtcgag
     6601 cgcataggac acggctttcc tcaagttttc gtccgcgaac ggtcctttcg cattgttgaa
     6661 tgcaagaacg gcgggagcat tggtggcggg aacggcgaca agtgaaacgg cgggactgga
     6721 agcaaggaaa tcctgatagg tggccggaac acccatggag tagttccaga cgatgtcgat
     6781 gtcaccgttc tggagcgcca gatacatggt atcctcattt ccaaagatcc ggtagacgat
     6841 ttctccaacc cttggagttt cgggataatc ggcattcgcc ttgaagacga tggttccgga
     6901 ttccgcggag aaggaatcaa aggtataagg accgcaaccg atgcgcttgt cggcgacggt
     6961 cggagaggcg ttgttttcaa agatgtgctt cggaagaatc ctgaaggagg tcatgtcgcc
     7021 aagcattctg acattggcgg agggaaggac aagcgtgatg cttcggttgt ccgcactgac
     7081 ggtacaggaa acatacttgg ctttggtaac cttgcctttg ctgtccgtct ggtcggtaag
     7141 atatgcgctg cctgaagcat cttcatgctg gaaggtgaag aggatatcct cggccgtaac
     7201 cgcgactccg tccgaccact tcagtccgtc tttcagggtg aaggtccagg aaacaccgtc
     7261 ctccgtcgcg aaatcgacaa gcagaggatg gtaggaaccg gaagtgtcct gatagacgag
     7321 aggaagctcg gtgaccgcgc tggccagcat atcgaaggcg tactcgcccc gctcggcctt
     7381 ctcgatcttg gcggttgttc cgacggtgat tctttccact gcaaaagaag agctttctcc
     7441 cgcaggagac tccttcacag gccgggcaaa aagcatcagc gcactggaaa gcaagatgat
     7501 gacgagtacc gataatctgt gtttcataat tccacctctt cactatttcc tgccatggtc
     7561 ggtgacacat ggacaaggat tgaatgatcg ctgttttact tccttcagga gcactatgca
     7621 taccggcata tgagggtagc ggtcacttca tgtgacgtgg aaacggcttc gtgccgtcga
     7681 tatcgagtat aagggtctcc aagcacgaga gtcaaccgac tcgattcttt tccagatcga
     7741 ggatccgatc cgaaacgtga tcaaccaaat ctctgtcatg gctgatgaga aggatggagc
     7801 cgcccttttc gatgacattc ttctttacaa gtgcaaatac attcgcctga accatcacat
     7861 ccagcatgct ggtagcctca tccatgatga gaagggtcgg ttgcagaaga aggcagcggg
     7921 caagagcgac cctttgggct tcgccaccgg aaatctgccg gggaagatgc gataggattc
     7981 cctcatcgag attgacttcg gaaagaaccc ttaaaatcaa ggtctccgtc tcttccttcc
     8041 cttttgccaa gccatgatac ctgatgagtt cggaaaagga acctctgatc ttctgattgg
     8101 gatcgaggga tgcataggga tgctggtaga ccatctggat tcctctcccc agtttcctgt
     8161 catacggcga agtcggagag aaaagcatgg ttccatcgat caggatctcc ccctcatcca
     8221 tgagaacaag tccgcaaagg atctttgcaa gggtcgtctt gccgatgccg cttttgccgt
     8281 agacccccag aatctctcct gtatcgagag aaaaactcag atcgctgagc acgtttttct
     8341 tcccgaacgc tttggagacg gacttcactt cgatcatccg ttgcaccacc attcgtgttc
     8401 cccttccctc tgatggatac gttcatccct gcaccgctcg gagtattccg gacagcggtc
     8461 ggaaaaagga cagcctgtcc tctttctcag cgtcggagtc gccttcatac cgttggaaac
     8521 gagcgaagag acgagacgct ttgtgtaggc attacgaggg ttctgaagaa agaaattcct
     8581 tccatactcc atggtcctcc ctccacagag gacaagaatc cgatccgcat tggaagcgac
     8641 cccgatgtca tgggtgatga atagttgtcc tgcgtcggga aacagaagtc caagatgctc
     8701 cagcatgcgg gatgccgctt ctttgtccaa accattcgtc ggttcatcgg ctatgatcag
     8761 tttcgccctg gtgcagagcg ccaaagccac ggtaacccgc tgagccatgc cgccggaaag
     8821 ctggaatgga tagctcttca gaatctcatc aatatcacga aatcctaccg agctgagggc
     8881 ggatgccgcc ttatccttcg cttcagcgct tctcacccct gcccggtgca ggctgtcgta
     8941 gagatgatgt ttgaccgttc tggaagggtt caggaactcc atgccgttct gaggaatata
     9001 cgccacttcc ctgcccagaa gagaagatgc gaccttggga tgagagcaat ccttcccgtc
     9061 aagaatacac tgcaagcctt cggtcttgac gttgggagca agtttgccca tcaaggaaag
     9121 agcgatcata gtcttgccgg aaccggtctc gccgatcagg gaaagcttct ccccttttcg
     9181 gatggaaaag gagacgtcct gcacaagggt cctgttcccg gccagcgtct tcgcatagat
     9241 ggttccactg gaataccgga gcagactctt ctctccttcc tctcttccct tcctttttga
     9301 agtcgccatg caatccatca taatgtaagt cctacagtat ttcatggcac aattcaagga
     9361 tgccttgcaa tccggacgtt tgaaatgatt tttccttctt tcaaggttct tggctctgac
     9421 ttctcccgcc ctcgaatgat tgacttcgtg gaagcttacc ggtggtttgg attccgtaca
     9481 taggcggtat tccttcccct gccttggcga aggatgtacc cctcatcctt gaggcgcttc
     9541 agggcggctt caaccgagga gcttccgata ctcggacatc ggtgaagaat ctccgtcttc
     9601 gtgaacactc ccaaggtgtt gtcgacggct ttgcgcacga tctcatacgg agtgttcttg
     9661 cggtccaccg ccccaagtct ctcttcaagg tctttatagc tgctgaggat gatgccaagg
     9721 aagtacttga tgaaggaagt gtaatcgttc ttttcctcat gccatccgac tgaagcatac
     9781 cccaacacat catagtacgt actctttgta cgttcgattt tctgctcgat gctgatgtac
     9841 ttgcccacca tgaaaccgct ttgatacagc aacaaggtgg taagcagcct gctcatcctt
     9901 ccgttgccgt cattgaacgg atggatgcac aagaaatcgc agatgaagat cgggatcaaa
     9961 atcagcggat tcaccatgtg ctcctccagg gctctccgat attgttcaca gatcgcatcg
    10021 accgccatcg gcgtcataaa cggttcgagc ggcgtgaagc ggatggaaga agttccatct
    10081 gcgtgaatct cgctgatatg gttccgggtg ttcttgaact tccccccgaa agtcatatcg
    10141 gtgtatgcaa gcagatcccg atgcagttgg aggaggacat cactcgtgag cgggatatgc
    10201 tcatagcttt cgtgaatcgt gcttagcaca tcacgatatc cggcaatctc ctgttcgtct
    10261 ctattccggg gtgcagtccg tttctccacc agctgtctca tccgtgcatt cgaagtaccg
    10321 ataccctcga ttctgttcga actgtcagtg ctctggatct ttgccacttc gacgagccgt
    10381 ctcagctcgg caggtttctc cctgaggtac aaatcctgtt tgcccttgta cacactgatc
    10441 tgggcgacaa aagagagtat ctctgcgtcc caagaggctt ccctcaaacc ataataatcg
    10501 aatgtacgca tctatctccc acctgatctg tcattctccc atattatact ctattttggg
    10561 agataatcaa tcgcggtggg agataacaca gtttttaggt acgtttccta aacgtctcaa
    10621 aatatcttcc tccagctcct tcagacgtgc attcaaagca ccgatgtaca aatcctgcct
    10681 gcccttgcac tcgctgatct tgcgacgaaa gagagcaccc tgcacccaag agcgttccct
    10741 gaacttgcag tggtcgaata tgcgcatcca tctcccacct tctgcatgat tctcccattt
    10801 tatgatgcta tatgggagat tgttggttca tatgcgagaa aaaacaggaa tcgtttgatg
    10861 gcttttccat cggaaatggg acaagtgtcg ccattttact ctcaaaacag ttgaaattac
    10921 ggaagggaac ccacacaata ttctcggagt gcatctctat gatcatcgtc aaaccggtcg
    10981 ttaccagaaa agaatggaat gcatttttcg cctttcccaa tgatctctac aaaggcaaca
    11041 agtactttgt cccctatctc atcgctgatg aaaaagacac cttctccccc aaaaaaaacc
    11101 cggcacatga gtattgcgat acgcagctct ttttggccta caaggatggc aaggtggtcg
    11161 gccgcatcgc cggattgatc aacaacaaac tcaacgcgat gaaagggatg aagcagatgc
    11221 gctttacccg ctgggatgtg attgacgata ttgaagtctc caaagccctt tttgaagcgg
    11281 tcttcgcttg gggcaaagca aagggaatga ccgagatcat cggcccgatc gggttcagcg
    11341 acctggacaa gcaaggcctg ctcgtcgagg ggtttgacca ggtcgggatg ttcatcaccc
    11401 tctacaacca cccgtattac catgagcatc tgacgaagct cgggttcgtg aaggatgtcg
    11461 actggattga atatacagtg tacaccccca aggaactcat tccccgagtg aagcgaatca
    11521 gtgaaatggc tcaggaacgc catggatatc ggcttttgga gtttaccaag aaaaaagaga
    11581 ttatccccta cgttcaagag atcttccaca tgtacaacga atcctatgcc cacctctacg
    11641 ggttctgccc cctcaccgat ggtcagatcg agatggcggt tgatcaattc ttcatggtgg
    11701 cccagctcga ttacatctac gtcgtggtgg ataaagagga tgcggtcatc ggcttcgggt
    11761 tgttgatccc ctccctttca aaggcgatgc agaagtccaa cggcagattg tttccgaccg
    11821 gactctttag gatcctccga tccctgaagc gctacgacgt gcttgacatg tacctgatcg
    11881 cggtcaagcc ggagtacttc ggccgcggag tgaacgccgt cattctgcat gaaggaatca
    11941 agaaagcgat tgaacacaac gtcgtgtacg cgaaaaccgg gcccatgttg gaaaccaaca
    12001 catatattcg gactcaatgg agaatcttcg agaccgagca gaataaacgc cgccgttgct
    12061 ttaaacaggt gttcaagtag gagaacaccc tcttcgccgc tcatcatctc tttgtctgca
    12121 taccctactt tggaagataa acagtttcca tatgagaaag aacaagaatt gctagatacc
    12181 ttttatcgga gagtgggaca agtgttatcg ttttcctacg aaaatagttg aaataaaaga
    12241 aggttaacta cacaatacgc ttggagtgta tagctatgat taccatcaag ccggt
//
LOCUS       DAOSLI010000049        11734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_5461_length_11734_cov_27.578902, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000049 DAOSLI010000000
VERSION     DAOSLI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5461_length_11734_cov_27.578902"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..721
                     /locus_tag="PLF67_07240"
     CDS             152..721
                     /locus_tag="PLF67_07240"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOE89838.1"
                     /translation="MDKAVIDFLRSLDRSLFLEGPSKRFADYDGPLPIGFNQTISQPS
                     LVAYMTDALEVDKTHKVLEIGTGSGYQTAFLAHFAKEVITIERIKELSVTAQERLHDL
                     GYHNITYIIGDGAKGWAELAPYDRIMVTAAPAEIPPLLVEQLRVGGKMVIPYGERTYQ
                     QLVLVKKVSDDSISRTKLLDVAFVPLISD"
     gene            938..1816
                     /locus_tag="PLF67_07245"
     CDS             938..1816
                     /locus_tag="PLF67_07245"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOE89839.1"
                     /translation="MNTPYVFTEIDRSLLNPLDSDEQSVILRKGTEYPYRGIYTDYTE
                     SGTYYCKQCNEPLYSSVAKFDARCGWPAFDKGLPNRIARVPDADGIRTEIICFTCGAH
                     LGHVFEGEGFTPTNTRHCVNSISMVFRPGEPVTTALYAGGCFWGTQYLFSKKSGVRTA
                     ESGYSGGTTENPTYQEVLTGKTGHYETVKVTYDPLKITYEELTKYFLEIHDPGQVDGQ
                     GPDIGKQYESVIFYRSRDQFDTAVKLIRRLETGGMKVATRVLPQKMFYRAESYHQDYY
                     RKKGTLPYCHYWTKRF"
     gene            1835..2530
                     /locus_tag="PLF67_07250"
     CDS             1835..2530
                     /locus_tag="PLF67_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOE89840.1"
                     /translation="MSERNLTFSTGCKDGFPIFIGYFPTAMAFGLVVKNLGLRLWEAV
                     LFSATNFAGSGQFLAASLIGSGALLAELFISVLLINLRYTFMGAEVARRLEDGITGMK
                     RALIAHGTTDEVFSISLMNPGRLPASYMAGLELTSYAGWVSGTALGFLVGLVLPPALQ
                     LAVGVTIYAMFSSLLAQEFRKKGIKVLLIAAISAGTHSVLTLLLGVKIGWSFVVSMLL
                     ASFIGSLIIREDV"
     gene            2527..2835
                     /locus_tag="PLF67_07255"
     CDS             2527..2835
                     /locus_tag="PLF67_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOE89841.1"
                     /translation="MKTFVVPILVSALATFIPRVIPYWVSFLDKLPPLLSRMLRLLPI
                     AAFGPLIFPGVFLDYVPHLYSGFAGISAAFILAYFKGGMILPILSSIVVTYLALLFGA
                     "
     gene            2837..3484
                     /locus_tag="PLF67_07260"
     CDS             2837..3484
                     /locus_tag="PLF67_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE89842.1"
                     /translation="MFDLHRHLSSASPSHACFYATSRKEEWETVIHAPYGAIGLLPSQ
                     LPAGVEELHALLVAHPHLQIGEVGLDRRFPHPDRQESFLKELLRVAVELERSVTLHCV
                     RREGRLLQILRGMERLPRLLWHSFGGSYEVAREAARLDIILSCGPRLKHSKLSGEGKR
                     LTRLPFGLESDHEKPTEDGYPETWQAHQRWFAELTDWSLDRLVRNNDEKRTILAH"
     gene            3456..4247
                     /locus_tag="PLF67_07265"
     CDS             3456..4247
                     /locus_tag="PLF67_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOE89843.1"
                     /translation="MRNEQFLRISRLLGDSAIKTLHDKTVTVVGLGAVGGMALESLVR
                     SGVGKVRIVDFDTIGITNLNRQILATYDTIGSEKTQVAKERIGAINPSCEVEVLPLFV
                     NKESVHRIFEGKETDLVIDAIDSLSPKVALLEYAYKANIPAISSMGAALRRDPFLVRK
                     ADLMDTWGCPLAKQVRYRLKRRGVGRGIDVIFSAEEIRFIYKDPSEEEHADFNERISS
                     TGRRRNVLGSLPTVTAIFGQHLAHLALTKLLGEAELSGVEVFNAR"
     gene            complement(4234..4821)
                     /locus_tag="PLF67_07270"
     CDS             complement(4234..4821)
                     /locus_tag="PLF67_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOE89844.1"
                     /translation="MAFTVYKGAAEPSLPIALVPLWGEYPNVEGSVRLGLRDTTLLLI
                     FKMRSPQLLRMVDRHNGPVYRDSCVEAFLQQQGRDEYLNFEFSASGSILAAKGKNREN
                     RTFFDPDLIDTIPLTIEILENNNRQSRYVVSVELDLRAFGLVEEGREVNLRGNFNACG
                     DGHKVPYYLAANPIGTVKPDFHAPAFFAPLVVTGR"
     gene            complement(4823..5236)
                     /locus_tag="PLF67_07275"
     CDS             complement(4823..5236)
                     /locus_tag="PLF67_07275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HOE89845.1"
                     /translation="MHIFPVRVYYSDTDCGGIVYHARYLDFAEHARTELLREIAQRHR
                     FDGNQSAMLNLSKTGFVVKSITIDYRKPAKLDDLLEVRTTVTSMKRFSMTLTQQVFKD
                     EDLIADLLVKVAAIHLDTLKPQPLGDDLQKAFLGG"
     gene            complement(5238..6503)
                     /locus_tag="PLF67_07280"
     CDS             complement(5238..6503)
                     /locus_tag="PLF67_07280"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOE89846.1"
                     /translation="MSKKTLPLGHGALLELAMELPTPFYLYDEEAIIENYRAFKEHFA
                     WAPNFKNYFAVKACPNPTIIRALAKEGSGVDCSSLPELLISEAVGLAGDDIVFTSNDT
                     PANEFKMAYELGAIINLDDITHIQFLEDTLGLFPETICFRYNPGPERTGNAIIGNPVE
                     AKYGVTSSQIIDCYRIAKKKGAKRFGLHTMVASNELDGTYIVETARMLFELCVRIQKE
                     AGVRISFIDMGGGIGIPYRPEDSAMDLAFVSREMKKLYEQIIVPAGLDPLSITFEVGR
                     MITGPYGYLVSKVLHVTEKYKDYVGLDASMADLMRPALYGAYHHITVLGKDREKHDHV
                     YDVTGSLCENNDKFAIDRSLPKVEVGDVVVIHDAGAHGHAMGFNYNAKLRSAEYLLKK
                     DGTIRLIRRAQTYDDYVATLRFDGAPVRI"
     gene            complement(6500..7729)
                     /locus_tag="PLF67_07285"
     CDS             complement(6500..7729)
                     /locus_tag="PLF67_07285"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOE89847.1"
                     /translation="MATINSAYRNLASGYLFPEINRRVRAWQERNPDKKVLRLGIGNT
                     TEALPPAICKAMIEKVEELGMRETYTGYGDEQGDGYLRKALVEYYQQGGVSLEMDEVF
                     VSDGAKSDAANIQTLFSTDSIVAVADPAYPVYVDSNVASGRSEGWDESRGMYRNLVYL
                     SCTEENGFIPPLPSEHVDLIYLCNPNNPTGAVMNHEEVKRFVDYARKHKSVIIYDSAY
                     SEYITEEGYPRSIYEIEGAKECAIEISSFSKFSGFTGVRLGWTIVPKSLVCEDSKPLE
                     LNRMWNRRQTTFFNGASNIAQRGGHAALSGEGYEQSRALVKYYLENARLIREGLKRVG
                     LTVYGGINSPYIWAKTPDGIDSWAFFDQLLDQCHVVVTPGGGFGPAGNRFVRVSAYNH
                     RENVEEAMKMIEERLKI"
     gene            complement(7732..8643)
                     /gene="dapA"
                     /locus_tag="PLF67_07290"
     CDS             complement(7732..8643)
                     /gene="dapA"
                     /locus_tag="PLF67_07290"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOE89848.1"
                     /translation="MKTIDFRGVHTALVTPFTAKDNLDEDALQAIIEHQIESGISGLV
                     PCGTTGESPTLSHDEHDRVIALTVKFANGRVPVIAGTGSNATDEAVRLSRHAQQVGSD
                     AVLLVSPYYNKPTQKGLFEHFKAIADSVDIPCILYNIQGRTAVNIETATVVALQEACP
                     NIVGVKEASGKLQQMQEVIDSSRPEFFVLSGDDNMCLPLIEIGGHGVISVASNLLPGY
                     VSTMVEKALNGDMAEAKKMEAVISNFFRTCFLETNPIPIKTAMARYGWCNESFRLPLC
                     TFESEAKRAELYRELNHLESLKIIKRR"
     gene            complement(8655..9404)
                     /gene="dapB"
                     /locus_tag="PLF67_07295"
     CDS             complement(8655..9404)
                     /gene="dapB"
                     /locus_tag="PLF67_07295"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOE89849.1"
                     /translation="MKLALVGYGKMGKIIKTHALAQNHEIASIIDPFEEGAKKSLTVE
                     NLHGCDVAIDFTHPDVALEHIRFYIENGVPAVIGTTGWYEHLGEVASMVDEANGTILY
                     SGNFSLGVALFLRSVRYAAALYGKTGRYDPAIIEVHHNKKADSPSGTAGMLASRVLEG
                     FPEKTRIEYETQHQRRGDEVLHVSSLRVGSVPGTHTVLFDSPEDTIELIHTARTRDGF
                     AFGAVAAAAWLNERESGLYTLEDMVDAVFLR"
     gene            complement(9489..11708)
                     /locus_tag="PLF67_07300"
     CDS             complement(9489..11708)
                     /locus_tag="PLF67_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HOE89850.1"
                     /translation="MKQLVLAEKPSVGRELARVLGCTQRSEGFIEGPEYIITWALGHL
                     IELAEPAAYSDSYRRWSLKDLPMLPPELKLEVIDQTKDQFRIIKSLVERADIASLIIA
                     TDAGREGELVARWIIEESGYRGPIKRLWISSQTEEAIQTGFANLKEGSLYDNLYRAAF
                     SRAAADWYVGMNVTRALTCHYDARLSAGRVQTPILALMVDREDEIEAFNGRFYWTLKA
                     QFGSISATLWPTEETSRIETEELANEYRARIVGKTGTVSDVYRDEKTIASPLAYDLTA
                     LQRDANTLLDFSAKETLDILQGLYERHKVVTYPRTDSRYITSDILPTLNRRIESLTNT
                     VLGPVARQYLTGGYGFDEGRFVQDAKVTDHHAIIPTEIRVNPASLSKKENDLFNLIAL
                     RFLEVLSSSYRYESILTTIESENLVFKSRSTRPLDLGWRRVALQSGLKSAEFVDEEEY
                     HTEEVAVGQILPVLDTALRRFTTPAPLRYTEATLLSAMENAGRLVDDPLLKKQMGGGL
                     GTPATRADIIEKLLQNAYIERDGKELVPTPKGRELIRLVPPSLKSAALTGEWELRLSN
                     IAEGIEEVGPFLSDIKESARSLVEEVKRSGEVFAPYFPESKTCPHCSSVMMKVTDPIG
                     QAHFICQRLSCSYEEKEIKKRVFIEPEKKDPPAPRKVVVVKAAANPEAVKKRVVVKKK
                     APEELSYRWETTIEVVRPSKLHRRAVPQQHVFKERESSGSSFADLIRASEERKKRRR"
BASE COUNT         2323 a         3445 c         3263 g         2703 t
ORIGIN      
        1 ctcctcggcc tcctcgtcgt cgctctccgc cctcagaaag tagttaccgt ccgaatgggt
       61 ccgacctacc cgtttaccgc tgaataaatc attgtaccga ggcgcagctc tctatctgcc
      121 aatcgtactg tgctgtggta tactttgcca tatggacaaa gccgtaatcg attttttacg
      181 ctcgctggat cgttcactct ttttagaggg tccgagcaaa cggtttgccg actacgacgg
      241 gccgctcccc atcgggttca atcagacgat cagtcagcct tcgctggtgg cgtatatgac
      301 ggatgcgctg gaggtggaca agacgcacaa agtccttgag atcggaaccg gcagcggata
      361 tcagacggcg tttctcgccc acttcgccaa agaagtgatt accattgaac gaatcaaaga
      421 gctctccgtt accgcccaag agcgtttgca tgacttgggt tatcataata tcacctatat
      481 tattggtgac ggcgccaagg gctgggccga gcttgctccc tatgaccgga tcatggtgac
      541 cgccgctccg gctgagattc ctccccttct ggtcgagcag cttcgcgtcg gaggaaagat
      601 ggtgatccct tatggcgaac ggacatacca acagctggtc ttggttaaga aggtctctga
      661 cgattcgatc agcaggacaa agctccttga tgtggcgttc gtccccctga tcagcgactg
      721 aaacccgaat ttccaccacc cgattctcct aagcgcaccg aagaggagga gcataatcga
      781 ggctcaaagc gacagtttgc gctgacattt cagaccggca acaggttttg cactcatctc
      841 actcctccgc ctttccttct ctttcgtttg ttcaagcaaa aaagcagtga cggtacccct
      901 tcttccttta ttattcatct cggagtacat tggtcgcatg aacactccat atgtatttac
      961 cgagatcgat cgatccctcc tcaatccgct cgatagcgat gaacagtcgg tcatcctgag
     1021 gaaaggaacc gaatatccct atcggggaat ctataccgat tacacggaaa gcggaaccta
     1081 ctactgcaaa caatgcaatg agccgctcta cagctcggtc gcgaagtttg acgctcgctg
     1141 tggatggccg gcgtttgaca aggggctgcc gaaccggatc gctcgagttc ccgatgccga
     1201 cgggatccga accgagatca tctgctttac ctgcggcgcg cacctcgggc acgtctttga
     1261 aggtgaaggc ttcactccga caaacacccg ccactgcgtg aattcgatca gcatggtctt
     1321 cagaccgggc gaaccggtca ccaccgcctt gtatgccggt gggtgcttct ggggaaccca
     1381 gtatctcttt tccaagaaaa gcggggtgcg gacagcggaa agcggataca gcggcggcac
     1441 gacggagaac cccacctacc aagaggtcct caccgggaag accggccatt atgaaaccgt
     1501 caaggtcacg tacgatccgc tgaagatcac ctatgaggag ctgaccaagt attttttgga
     1561 gattcatgat cccggtcagg tggatgggca aggaccggat atcggaaagc agtacgaatc
     1621 cgtcatcttt tatcgcagcc gcgaccagtt tgacactgca gtcaagctga tccgtcggct
     1681 cgaaacgggg ggtatgaagg ttgcaacccg ggtgcttcct caaaagatgt tctatcgggc
     1741 ggaatcctac catcaggact actaccggaa aaaaggaacc ctgccatact gccattactg
     1801 gacaaaacgg ttttaactta ctacgctcac gtttatgagc gaacgcaatc ttaccttctc
     1861 tacgggctgt aaagacggct ttccgatctt catcgggtat tttcccaccg cgatggcgtt
     1921 tggcctcgtg gtaaagaatc tgggattgcg tctttgggaa gcggtgctct tttccgccac
     1981 gaactttgcc ggcagcggcc aattcctcgc agccagcctg atcggatcgg gcgccctcct
     2041 tgccgaactg ttcatcagtg tcctgctcat caaccttcgg tacaccttca tgggagcgga
     2101 ggtcgcccgg cggctggagg atggaatcac ggggatgaag cgggccctca tcgcccacgg
     2161 aacgaccgat gaagtgtttt ccatctcgtt gatgaatccc ggaaggcttc ccgcctcgta
     2221 catggcgggc ttggagctga cctcctacgc cggatgggtc agcggaacag cgctgggctt
     2281 cctcgtcggt ctggtactcc ctcccgccct tcagctggcg gttggagtta cgatctatgc
     2341 gatgttctca tcgctgttgg ctcaggagtt tcgtaaaaaa ggcataaagg tccttctcat
     2401 cgccgcgatc tcggcgggaa cccactcggt tttgacgctg cttcttggag taaagatcgg
     2461 ttggtcgttc gtcgtctcga tgctccttgc aagcttcatc ggctcgctca ttattcggga
     2521 ggatgtatga aaacattcgt ggttccgatc ctcgtctccg cgcttgcgac cttcattccc
     2581 cgggtcatcc cctattgggt ttcctttctg gacaagctgc cgccgctgct ttcccggatg
     2641 ctccgtctcc ttccgatcgc cgccttcggg ccgctgatct ttcccggggt gttcctcgat
     2701 tatgttccgc acctctactc ggggtttgcc ggaatttccg ccgccttcat cctcgcatac
     2761 ttcaaaggcg gaatgattct tccgattctc tcctcgatcg tggtaacata tcttgcactc
     2821 ttgttcggag cataggatgt tcgacctgca ccgccacctt tcttcagctt ctccatcaca
     2881 tgcctgtttc tatgcgacaa gcaggaaaga ggagtgggaa acggtgatcc acgcccccta
     2941 cggggcgatc gggctcctgc cgtcgcaact gccggccggt gttgaagagc ttcatgctct
     3001 tctcgtcgcc cacccgcatc tccagattgg agaggtcggt ctcgaccgaa gatttcccca
     3061 tcccgatcgg caggaatcgt ttctaaaaga actcttgagg gtcgccgtcg aactggagcg
     3121 cagcgtaacg cttcactgcg ttcgaagaga agggcggctg cttcagattc taaggggcat
     3181 ggagaggctt ccccgtctcc tgtggcactc cttcggcggc agctatgagg tcgcccgtga
     3241 agccgctcgt ctggatatca tcctcagctg cggaccgagg ttgaagcatt caaagctgag
     3301 cggcgaagga aagcggctca cccggcttcc gttcggcctt gaaagcgatc atgaaaagcc
     3361 gacggaggat ggatatccgg aaacctggca ggctcatcag aggtggtttg ccgaattgac
     3421 ggactggagc cttgaccgat tggtgagaaa caacgatgag aaacgaacaa ttcttgcgca
     3481 ttagccggct gctcggcgac tcggcgatca aaacccttca cgataagacc gttaccgtcg
     3541 tcggtctcgg agcggtcgga ggaatggctt tggagtcgct cgtccgcagc ggggtcggca
     3601 aggtgcgcat cgtggacttt gacacgatcg ggatcaccaa cctcaaccgg cagattcttg
     3661 cgacctacga tacgatcggg agcgaaaaga cgcaggtggc gaaagagcgg atcggcgcga
     3721 tcaatccttc ctgcgaggtt gaagtacttc ctcttttcgt gaacaaggag tcggttcatc
     3781 ggatctttga aggcaaagag acggatctgg tcatcgacgc gatcgattcg ctcagcccga
     3841 aggtcgcgtt gctcgagtat gcgtacaagg cgaacattcc cgcaatttca tcgatgggtg
     3901 ccgcccttcg gcgcgatccc ttcctggtgc gcaaggcgga tctcatggat acgtgggggt
     3961 gtccgctggc caagcaggtg cgttaccgcc tcaagcgcag gggcgtcggc cgggggatcg
     4021 atgtaatctt ttcagcggaa gaaatccgct tcatctacaa agacccgtcc gaagaagagc
     4081 acgccgattt caatgagcgg atcagctcaa caggccgcag gcggaacgtc cttggatccc
     4141 ttcccaccgt caccgcgatc ttcggccagc acctcgccca ccttgccctg acgaagctcc
     4201 ttggtgaagc ggagctttcg ggagtggaag tgttcaacgc ccggtgacga cgaggggagc
     4261 gaagaaggcg ggggcatgaa aatccggctt caccgttccg atcgggtttg ccgccagata
     4321 gtagggaacc ttgtgcccgt cgccgcacgc gttgaagttt ccccgaagat tcacctctcg
     4381 cccttcttcg acgagaccga acgctctcag atcgagctca acggaaacga cataccgact
     4441 ttgacggttg ttattctcca gaatttcgat cgtcagaggg atcgtatcga tcagatcggg
     4501 gtcgaaaaat gttcggtttt cgcggttttt ccctttggcg gcaaggatgc tccccgaggc
     4561 gctgaactcg aagttcagat actcgtccct tccctgttgc tgtaaaaacg cttccacgca
     4621 gctgtccctg tacacgggtc cattgtgtct gtccaccatc ctcaaaagct ggggggatcg
     4681 catcttaaag atgagcagca aggtcgtatc ccgaagcccg agccgcaccg acccttcgac
     4741 gttcggatac tccccccaga gcggaacaag cgcaatcggg aggcttggtt ccgctgctcc
     4801 cttatacacg gtaaaggcca catcaccctc caagaaacgc tttttgaaga tcatcaccca
     4861 acggctgagg cttgagtgtg tccaagtgga tcgccgccac ttttaccagg agatcggcga
     4921 tcagatcctc gtccttgaag acctgctgcg taagcgtcat cgaaaaccgc ttcattgaag
     4981 tgaccgtcgt ccgtacttca agcaagtcgt ccagcttggc gggcttccga taatcgatcg
     5041 tgatcgattt caccacgaag ccggtcttcg acagattcag catcgccgat tgattgccgt
     5101 cgaacctatg ccgctgtgcg atctcgcgaa gcagttcggt ccgggcgtgc tcggcaaaat
     5161 cgagataccg ggcgtggtag acgatcccac cacagtcggt atcgctgtaa tacaccctga
     5221 cgggaaagat gtgcatctca gatcctcacc ggtgcgccgt cgaagcgcag tgtcgctaca
     5281 taatcatcgt atgtttgcgc cctccgaatc aaccggatgg tgccgtcttt cttcaagaga
     5341 tactccgcac tgcggagttt cgcgttgtag ttgaacccca tcgcatgccc gtgcgccccg
     5401 gcatcgtgga tcacgacgac atcccccacc tcgaccttcg gaaggcttcg gtcgatcgcg
     5461 aacttgtcgt tgttttcaca gagcgagccg gtgacatcat agacgtggtc atgcttttcc
     5521 cgatccttgc cgaggacggt gatgtggtgg taggccccat acagcgccgg ccgcatcaga
     5581 tccgccatcg atgcatccag gccgacgtag tccttgtact tctccgtcac gtgcagaact
     5641 ttactgacca gatagccgta aggccccgtg atcatccttc cgacctcgaa ggtgatggaa
     5701 agcggatcga ggccggcagg aacgatgatc tgttcgtaga gctttttcat ctcccgtgaa
     5761 acgaaggcaa gatccatcgc cgagtcttcc ggtcgatagg gaatcccgat tcccccgccc
     5821 atgtcgatga aggagattct cacccccgct tccttctgaa tcctgacaca gagctcgaag
     5881 agcatccggg cggtttcgac gatataggtg ccgtccagtt catttgaagc caccatcgta
     5941 tggagtccga agcgcttcgc ccccttcttt ttggcgatcc tgtagcagtc gatgatctga
     6001 cttgaggtaa ctccgtactt cgcctcgacg gggttgccga tgatcgcatt tccggtgcgc
     6061 tcggggccgg ggttgtaccg gaaacagatc gtttcgggga acaggcccag ggtatcctca
     6121 aggaattgaa tgtgggtgat gtcatccaga ttgatgatcg cccccagctc atacgccatt
     6181 ttgaactcat ttgccggagt atcgttggag gtgaagacga tatcatcccc cgcaaggccg
     6241 accgcttcgc tgatgaggag ctcgggaaga ctggaacagt caaccccgct tccctccttc
     6301 gccaacgctc tgatgatcgt cgggttgggg cacgctttca ccgcaaaata gttcttgaag
     6361 ttcggcgccc acgcaaagtg ctccttgaac gcccgatagt tctcaatgat cgcctcttca
     6421 tcatagaggt agaacggagt cggcagctcc attgcaagtt ccagaagcgc gccatggccc
     6481 aatggcaggg tctttttgct catatcttca acctctcttc aatcatcttc atcgcttcct
     6541 cgacgttttc gcggtggttg tacgccgata cccggacgaa cctgtttccc gccggcccga
     6601 agccgccgcc tggggtcacc acgacgtggc actgatcgag cagctgatcg aaaaacgccc
     6661 agctgtcgat tccgtccggg gtctttgccc agatgtaggg actgttgatt ccgccgtaga
     6721 cggtaaggcc gacccgctta agaccttccc ggatcaggcg ggcgttttcc agatagtact
     6781 tcacaagagc ccggctctgc tcgtaccctt ccccggaaag cgccgcatgg ccgcccctct
     6841 gggcgatgtt gctcgcaccg ttgaaaaagg tggtctggcg gcggttccac atcctgttga
     6901 gctccagagg cttgctgtcc tcgcagacaa ggctcttggg gacgatcgtc cacccgagcc
     6961 gaaccccggt aaatccggag aacttggaaa acgaggagat ttcgatcgcg cactccttcg
     7021 ccccctcgat ctcgtagatc gaacggggat atccctcttc ggtgatgtat tcgctgtagg
     7081 cgctgtcgta gatgatgacg ctcttgtgtt ttcgggcata gtcgacgaac cgtttcacct
     7141 cttcatggtt catcaccgcc ccggtggggt tgttcgggtt gcacaggtag atcagatcga
     7201 catgctcgga gggaagcggc ggaatgaatc cgttttcttc cgtgcaggag aggtagacaa
     7261 ggttcctgta cattccgcgg ctctcatccc atccttcgga gcggccgctt gccacgttgg
     7321 aatcgacgta gaccgggtac gccggatcgg ccaccgcgac gatcgaatcg gtcgaaaaga
     7381 gggtctggat gttcgcagcg tcgcttttcg ctccgtcgct gacgaacact tcatccatct
     7441 ccaaagagac gccgccctgc tggtagtatt cgaccaaggc ctttcgcaga tatccgtcac
     7501 cctgctcgtc tccgtagccg gtgtaggtct cccgcatccc cagctcttcc accttctcga
     7561 tcatcgcctt gcagatcgcc ggaggaagcg cctcggtggt gtttccgatc cccagccgaa
     7621 gcaccttttt atcgggattt cgctcctgcc acgcgcggac tctgcgattg atctcgggaa
     7681 agagatatcc gcttgccagg ttcctgtatg cgctgttgat cgtcgccatc tctacctcct
     7741 cttgatgatc ttcaaggatt ccaagtggtt caattcccga tagagctccg cccgcttggc
     7801 ttcgctttca aaggtgcaga gcggcaggcg gaacgattcg ttgcaccacc cgtatcgggc
     7861 catcgccgtc ttgatcggaa tcggattggt ctccaggaaa caggtgcgga agaagttcga
     7921 aatgaccgct tccatctttt tcgcttcggc catatctcca ttgagcgctt tttcaaccat
     7981 cgtcgagaca tatccgggaa ggaggttcga agcgacgctg atcaccccgt ggccgccgat
     8041 ctcgatcagg gggagacaca tattgtcatc cccgctgagg acgaagaact ccggcctcga
     8101 ggagtcgatc acctcctgca tctgctgaag ctttccgctc gcttccttca ctccgacgat
     8161 attggggcat gcttcctgaa gtgcgactac cgtcgccgtc tcgatgttca ccgcggttcg
     8221 gccctggatg ttgtagagga tgcagggaat gtcgacgctg tcggcgatcg ccttgaagtg
     8281 ctcgaagaga cctttctggg tcggtttgtt gtaatagggg ctgaccagca ataccgcatc
     8341 ggaaccgacc tgctgggcgt ggcgggagag ccggaccgcc tcatcggtcg cgttcgaacc
     8401 ggttccggcg atgaccggca cccttccgtt cgcaaacttg accgtcaggg cgatgacccg
     8461 gtcgtgctcg tcgtgactga gggtcggcga ctcgccggtc gttccgcagg gaacgaggcc
     8521 gctgattccg ctttcgatct gatgctcgat gatcgcctgc agggcgtcct catccagatt
     8581 atcctttgcg gtgaacgggg taaccagtgc ggtgtgcact cccctgaaat cgattgtttt
     8641 catcgtgttg tctcctatcg cagaaatact gcgtcaacca tgtcttccaa tgtatacagc
     8701 ccgctttccc gttcgttcag ccacgctgcg gcggccaccg cgccaaaggc gaagccgtcc
     8761 cgggttcgag cggtgtggat cagctcgatc gtgtcctccg gactgtcgaa gaggacggta
     8821 tgggtgcccg gcaccgaacc gacccggagg cttgatacgt gcagcacctc gtccccccgt
     8881 ctttggtgct gggtttcata ttcgatccgg gtcttttcag gaaagccttc cagcacccgg
     8941 gaggcgagca tgcccgccgt tcccgaaggg ctgtccgctt ttttgttgtg atgcacttcg
     9001 atgatcgccg gatcgtagcg tcccgtcttg ccatacagag ccgcggcata tcgtacgctc
     9061 cgtaaaaaga gcgcgactcc caaagagaaa ttcccgctgt agaggatcgt cccgttcgct
     9121 tcatcgacca tggaagcgac ttcaccaagg tgctcatacc acccggtggt cccgatcacg
     9181 gcgggaaccc cgttttcgat ataaaaacgg atatgctcca acgccacatc gggatgggtg
     9241 aagtcgatcg ccacgtcaca gccatgcagg ttttcgacgg tcaatgattt cttcgccccc
     9301 tcttcaaagg ggtcgatgat cgaagcgatc tcatggtttt gagcaagggc gtgggttttg
     9361 atgatctttc ccatctttcc gtacccgaca agcgcaagct tcataggctt cctcccgcat
     9421 ggagagtagc ctagcagaaa gctacaagag tgtctacagc gtaacagaat gtttttataa
     9481 taacagtatt accgtcttcg cttcttccgc tcctcgctcg ccctgatcag gtcggcaaag
     9541 gaggatccgg aggattcccg ctccttgaat acatgctgtt ggggtaccgc tcggcggtgc
     9601 agtttggagg ggcggacgac ctcgatcgtc gtctcccatc ggtaggaaag ctcttccggt
     9661 gccttcttct tcaccaccac ccgcttcttc accgcttcgg gattggcggc cgctttgacg
     9721 accactacct tcctgggggc gggaggatcc ttcttctccg gctcgatgaa cacccgcttt
     9781 ttgatctctt tctcctcata ggaacaggag agccgctggc agatgaagtg cgcctggccg
     9841 atcgggtcgg tgaccttcat catcacgctc gagcaatggg ggcaggtctt cgattcggga
     9901 aaatagggag cgaacacctc gccgcttcgc ttcacttctt cgacaagcga gcgggcgctc
     9961 tccttgatgt cggagagaaa cggtccgacc tcttcgatcc cttcggcgat attggagagg
    10021 cgcaattccc attcgccggt gagggcggcg ctcttcaggc tggggggaac cagccggatg
    10081 agctcccgcc ctttcggggt gggaaccagc tccttgccgt cccgttcgat gtaggcgttc
    10141 tggaggagct tttcaatgat gtccgcccgt gttgcggggg tgccgagccc tccgcccatc
    10201 tgtttcttca gcagcgggtc atcaacgagg cgtccggcat tctccatcgc cgagagcagg
    10261 gtggcttccg tgtagcggag gggcgcaggc gtggtgaatc gcctgagcgc ggtgtcaaga
    10321 acgggaagaa tctgtccgac cgctacttct tccgtatggt attcctcttc atccacgaac
    10381 tcggcgctct tcaggccgct ttgcagggcg acccttcgcc accccaagtc gagaggccgg
    10441 gtcgaccggc ttttgaagac gagattctcg ctttcgatcg tggtgaggat cgactcgtag
    10501 cgataggagg aggaaagcac ctccaggaag cggagggcga tcaggttgaa gagatcgttc
    10561 tctttctttg ataaggaagc aggattcacc cgtatttcgg tggggatgat cgcatggtgg
    10621 tcggtgacct tcgcatcctg gacgaagcgc ccttcatcga agccgtatcc cccggtgagg
    10681 tactgccggg cgaccggacc caaaacggta ttggtcaacg attcgatcct ccggttgagg
    10741 gttggcagaa tgtcggaggt gatgtagcgg ctgtcggttc ggggataggt caccaccttg
    10801 tgccgctcat agaggccttg gaggatgtcc agcgtttcct ttgcgctgaa atccaaaagg
    10861 gtgttggcgt cccgctgaag ggcggtcaga tcgtacgcca gaggggaggc aatcgtcttc
    10921 tcatcccgat atacgtcgct caccgttccg gtcttgccga caatcctcgc ccggtattca
    10981 ttggccagct cctcggtctc gatccggctg gtctcttcgg tgggccacag ggtggcggaa
    11041 atcgaaccga actgggcttt caaggtccag tagaagcggc cgttgaaggc ttcgatctca
    11101 tcttcgcggt cgaccatcag cgcgagaatc ggggtctgca ccctgccggc ggagagccgg
    11161 gcgtcgtagt ggcaggtaag agcccgggta acgttcattc cgacgtacca atccgccgcg
    11221 gcccggctga acgccgcccg gtagaggttg tcatacaggc ttccctcttt caggttggcg
    11281 aatccggttt gaatcgcctc ttccgtctgg ctgctgatcc acaggcgctt gatcgggccc
    11341 cggtagccgg actcttcgat gatccagcgg gcgaccagct ccccttcccg gccggcatcg
    11401 gtggcgatga tcagggaagc gatgtccgcc cgctcgacga gggacttgat gattcgaaac
    11461 tgatctttcg tctgatcgat cacctcgagc ttcaactcgg ggggaagcat cggcagatcc
    11521 ttcagcgacc accgcctgta ggaatcgctg taggccgccg gctcggccag ttcgatcaga
    11581 tggcccagcg cccacgtgat gatgtactcc ggcccttcga taaacccttc actgcgctga
    11641 gtgcacccca gcacccgggc aagctcccgc ccgacactcg gcttttccgc caacaccaac
    11701 tgtttcaaag attcctccac cttcgtgatg atac
//
LOCUS       DAOSLI010000050        11129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_5788_length_11129_cov_33.994853, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000050 DAOSLI010000000
VERSION     DAOSLI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5788_length_11129_cov_33.994853"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..336)
                     /locus_tag="PLF67_07305"
     CDS             complement(154..336)
                     /locus_tag="PLF67_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89851.1"
                     /translation="MVKEYTMVYLNKGFKLSREKDLGQAQTIINEYVAKGWQLQQVVS
                     PSDMSGVLVGVFYKEH"
     gene            complement(529..678)
                     /locus_tag="PLF67_07310"
     CDS             complement(529..678)
                     /locus_tag="PLF67_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89852.1"
                     /translation="MRIPDDHLPPYRQEEIFRREGIFLTRQKLASRVIIYYERLQVPN
                     VKGRC"
     gene            752..1411
                     /gene="deoC"
                     /locus_tag="PLF67_07315"
     CDS             752..1411
                     /gene="deoC"
                     /locus_tag="PLF67_07315"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOE89853.1"
                     /translation="MKQEDIAPYIDHTVLAADATKQRIEQLCKEAIKYNFASVCVNGC
                     WVKTCAKLLEKSSVKVCTVVGFPLGAMSSEAKAYEAKKAISAGASEIDMVINIGFLKD
                     RQDDKVQEDISAVKAVCGRAAILKVIIETSLLNDEEKARACRLAKASGADFVKTSTGF
                     STGGATVKDVALMRKTVGPDTGVKASGGVRTYEDFLAMVGAGANRIGTSNGVAIINGA
                     H"
     gene            1398..2540
                     /locus_tag="PLF67_07320"
     CDS             1398..2540
                     /locus_tag="PLF67_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOE89854.1"
                     /translation="MAHIEQSPQARDFLKSLGFPTLAFHETFSHFDFTVPGRRVLLIG
                     PMGSGKTEFAARVWRDAAIAQKKGKVVQKQTSTGEVDRRNVFFVRAQIDASRFPDAPP
                     DAMVYRNGYIRCGENIALIRDSFDLERVLADNPKVGTFIIDEASFFDERLAYVVRNHS
                     YERGVMFIFPSLILNFRRDLFNMTARLMLDIATDVIPLTAYCEHPDCLRDAFYTYRYY
                     QVGEKECPALYFDPLIIVGGDEHKDSSVEPNYAARCDEHHYLPGKEYTYFILKPLGQK
                     AAAGDEKPLINELTTLKEEIKASQLWKNFTERYEKLNDKEILFNSLEVEKIAEKALLY
                     LFVEQNLIPEDLLLRIVDLLELDVAYMSKVLTDNKRPVSFAQPLLF"
     gene            complement(2601..3470)
                     /locus_tag="PLF67_07325"
     CDS             complement(2601..3470)
                     /locus_tag="PLF67_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE89855.1"
                     /translation="MMRFGVRAHDLGSFDTPEALAAAVRTFGEHLPVQLAVAKSFPSS
                     GPLDEAFALRIREAFDRQHVSIAVLGCYINPIHPDPAERERQLKRFEEHLALASILGA
                     PMVGTETGSVRPDCSYHPDTAEPKHLETLYSSLDRLVEYAVKVGSVVAVEAVAKQNTI
                     CSIERMARLAERFDTPRLKIIYDPVNLIPWIGIPEADGSFRARPSLEAQRAFTDSALD
                     AFGLRIAALHVKDFVLDEHGFKIGNLPALEGVFDWPALLADLRSRKISVPALLENANP
                     ATLESTLSTFYSL"
     gene            3517..4869
                     /locus_tag="PLF67_07330"
     CDS             3517..4869
                     /locus_tag="PLF67_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOE89856.1"
                     /translation="MATMRATTLIDGGTYNNTERFQTLFDDLTEGDELVIPSGVYATG
                     ALTLKKHNVTITLEKGCRIQFIPDPELYPPVYTRWEGVNCWAMQPALLITDSSHIRLR
                     GEGILDGSGSWWWQQAVVKKKQSKPITDLEHSFAHLNPGFEEQASGGGGRKSQFLRPP
                     LLQIHQSRNVHISGITLQNSPFWTLHPLYSKRVTIEDVTIFNPEDAPNTDGIDIDSCR
                     DVVVRNCIVDVGDDGIAIKSGSGSDGVVTNQPTVGVVIEGCTVRSAHGGAVIGSETAA
                     GVRDVLVQGCIFDGTDRGIRIKTRRGRGGEITNIHYRSLTMKDNLCPIAVNMYYRPGA
                     TNPDLFSLDPQPILDTTPSISNILIEECTAVGATSSAGFIVGLPEEPIRNVRIVRSSF
                     GVSNENVTAIEESEMYDGLPALRERGIRLRNVHLHLEDVKVLGVKEGFVVEEGVTFDS
                     "
     gene            complement(4866..5828)
                     /locus_tag="PLF67_07335"
     CDS             complement(4866..5828)
                     /locus_tag="PLF67_07335"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOE89857.1"
                     /translation="MSDTLMKKIQLIGLVPVVKIDDAAKAVPLAKAMIAGGLPVAEVT
                     FRTAAAEEAISNIAKAVPEMLLGAGTVTSVEFAKKAVKAGAKFIVSPGFNPEVVDWAL
                     ENNVPIVPGVCTPSDIEAGLSRGLSTLKFFPAEASGGVDMLKNFAGPFSNVMFMPTGG
                     INAKNLGSYAALSNVLAVGGSWMVKSDLIENEQWDEITTLCAEAVLALQGLRFAHLGI
                     NETTKENAQVAAASLELLGMTRKVGNSSTFLDTVIEVTHAPFYGKHGHLGFACNNVDR
                     TVHYLQKKGFTLNEESVKRDDKGAMKSCYFNEEIAGFAFHLIKG"
     gene            5932..6969
                     /locus_tag="PLF67_07340"
     CDS             5932..6969
                     /locus_tag="PLF67_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOE89858.1"
                     /translation="MSKKFVTFGEIMLRLKSPGHERFFQSPALEATFGGGEANVAVSL
                     SLLGKKAQFVTALPKNAIGEAAVREVRRYGVDTSAILRTEEGRVGIYFLETGANQRPS
                     LVVYDRAESSIATAKPSDFDFAKILKDAAWLHVTGITPAVSAEAAASTLAALKEAKKQ
                     GVTVSIDLNYRKKLWKWGKTAVEVMRELATYADVIVANEEDIQKCLGIEANVDVTSGE
                     LDTNVYKALTEEVKKQFPNISVIGVTLRESRSADVNGWSAAINGKTGFYLSRSYEITD
                     IIDRVGGGDSFSAGFIYALSEYGDDEEYVINFAVAASALKHSISGDFNLTTLSEVKAL
                     VKGDGSGRVQR"
     gene            complement(6935..8224)
                     /locus_tag="PLF67_07345"
     CDS             complement(6935..8224)
                     /locus_tag="PLF67_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE89859.1"
                     /translation="MTPLEKLAKAKRFDMKRKPIPQRHYLRPLTWLLSYPEVWLRRLK
                     IRQRGMEGIRPPFLLLCTHMAFIDFKVTTAALFPHRANYVVAIDGFIGREWLLRNAGG
                     ICKRKFTNDLQVVRQIKEVLHVNGDVLALYPEARYSLCGTTAVLPDSLGKICKLLGVP
                     VVMLNMHGHYLNSPVWNLKKRGVPIACDYFLIADAQETESLSVDELNERIRSAFEYDE
                     YRRQKENGIAITFKKRAEGLHKILYQCAHCHTEYEMHSFGSRIACKACKKEWEMGIYG
                     ELGASKPAEDETALVTEFSHIPDWYEWERSNVRREVEKGTYSLDVPVMIEALPNARGY
                     IPIGIGRLVHDMKGFTLETEGLFLHKDVSSMYSCHIEYEYKGKGDCIDLSTLDDTYYL
                     YPKEKPFAVTKVALATEELFFHAKKTSSAEPDPIRPL"
     gene            8329..9411
                     /locus_tag="PLF67_07350"
     CDS             8329..9411
                     /locus_tag="PLF67_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89860.1"
                     /translation="MDKDLLTELYTLAKATFAANLDGLFFEEDLFAVSLANRPTYYVT
                     LGNGVLVANRGRKGLTNFLEYLEHEETEDELEFFEYQQAMDSLYSVVPFDEDDDMLET
                     DETWRKESGVLFEDMAAPIFRSKVEYCLPGIIDEEEAKEFIRIFKATLHLIARLTEIK
                     KEHPKHRAIAMWHDQVSDGSKKNEYIPRLTVGENEILLDKLLFEPWNYENTFPVPEFA
                     DAEQRRYYKSMKAKSGKVFYMATGIFPAPVSSEKFDEPTFPVFYLLYDPQAHQILDVY
                     MLDDYEKDHNIFIDRFLAVVDRGVKPQAIHCYGRRTLPLLTKIGPNMGVMVFEGKRDA
                     ELDEVIDDMLHHFLHDGIEEEDEHDR"
     gene            complement(9408..10385)
                     /locus_tag="PLF67_07355"
     CDS             complement(9408..10385)
                     /locus_tag="PLF67_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE89861.1"
                     /translation="MKTIRWGIIGCGDVTEVKSGPGFQKARGSELVAVMRRTGEKAKD
                     YARRHNVPRWYDDADQLIGDPEVDAVYIATHPDTHKEYALRVARAGKPVYVEKPMGLN
                     YQHGLEMVQGCARANVPLFVAYYRRALPKFLRVKKILESKRLGEIRAVEVLMRQQIKK
                     RDTQDGGSWRVRPEISGGGHFPDVGSHALDLIDFLLGPIVEAQGMGANQSKTYRAEDI
                     VWGHFKTHQGVAGVGTFLFNTYTDDDWTTIHCEKGDLAYSVLDLNQPIVIRTKEGTET
                     IAGDPPPAHVAQPFIQTVVDELLGIGTCPSTGESALRTDWVLARLAGTI"
     gene            complement(10560..>11129)
                     /locus_tag="PLF67_07360"
     CDS             complement(10560..>11129)
                     /locus_tag="PLF67_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002676904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HOE89862.1"
                     /translation="SPKAEPFKMWLAEVGKERIDEIVDPERTIDRALQTYLRKGYSRE
                     WINQRLQAIQVRKELTDAWGDHGVREGVEYAILTDEISKAWSGMTTRQYKDFKNLKKE
                     NLRDNMSTLELVLTMLAEATTTELTQTLNPHGLEENKEIAKEGGTVAGNARKDIEART
                     GRSVLTQKNALDFSKLIEDVADKTEDHST"
BASE COUNT         2520 a         2914 c         3128 g         2567 t
ORIGIN      
        1 gagtaaaagc gttcaatcgt ttcaagaatc atagaggtag ggtattggca gcgggaatcc
       61 tcgtcaagat attctccaat gtagcttctt atttaattgc ttgatgttta attggttgag
      121 tttgataaac tgggatgtat tgtgcaaatt acatcaatgt tccttataga aaactcccac
      181 aagaactcct gacatgtcac ttggcgatac aacttgctgt agctgccatc ctttagctac
      241 atactcattt ataatggttt gagcctgccc taaatctttc tcacgtgaaa gtttaaatcc
      301 tttgttcaaa tagaccatag tatattcttt caccactatt acctccatta attctaatgt
      361 atctcactaa atattcagca atggtatacc atttttaagc tgtttttttc taccatcaga
      421 cttcatgctt gttttcaagt ttttataaaa gcagataggg aacgaatgga aagggtagat
      481 agaaaagtcg tatgttttct ttattctttc ttttcagaat atatcacttt aacagcggcc
      541 tttcacattc ggcacctgaa ggcgctcata gtagatgatg acccggcttg caagcttctg
      601 cctggtgaga aagattccct ctcttctgaa gatctcttcc tgacgatacg ggggaaggtg
      661 gtcatcggga atcctcatca aggtattctc caattgcccg ctcatcggat ttggagtaag
      721 ctgtcactac gttacttgat ggaggatctg gatgaaacaa gaggatatcg ccccatatat
      781 cgatcacacg gttctcgccg cggatgcgac gaagcagcgc atcgaacagc tgtgcaaaga
      841 ggccatcaaa tataacttcg cctcggtgtg tgtcaatggg tgctgggtca aaacgtgcgc
      901 caagctgttg gaaaagagct cggtgaaggt gtgtaccgtg gtcggattcc ccttgggggc
      961 aatgagcagt gaagcgaagg cgtatgaggc gaagaaagcg atcagcgccg gggcgagtga
     1021 aatcgacatg gtcatcaaca tcggatttct gaaagaccgc caggatgaca aggtgcagga
     1081 ggacatcagc gcggtgaaag ccgtctgcgg ccgggcggcg atcttgaaag taatcattga
     1141 aaccagtctg ttgaacgatg aagaaaaggc tcgcgcctgt cgccttgcca aggccagcgg
     1201 agcggacttc gtaaagacct cgaccggatt ttccaccggt ggagccaccg tgaaagacgt
     1261 tgcgctgatg cgcaagaccg tcggtcccga tacgggagtg aaagcttcag gcggggttcg
     1321 aacctacgaa gatttccttg cgatggtcgg agccggtgcg aatcgaatcg ggacttccaa
     1381 cggagtggcg atcatcaatg gcgcacattg aacaatcgcc tcaggcccgg gattttctca
     1441 agagcctggg ctttccgacc cttgcgttcc atgaaacgtt ctcccacttc gatttcaccg
     1501 tgcccggacg gcgggttctc ctgatcgggc cgatgggctc ggggaaaacc gagttcgccg
     1561 cccgggtctg gcgggatgcg gcgatcgctc aaaagaaggg gaaggtggtt cagaaacaga
     1621 catcgaccgg cgaggtcgat cgacgaaacg tcttttttgt ccgcgcccag atcgatgctt
     1681 cccgctttcc ggatgcaccg ccggacgcga tggtctaccg aaacggctat atccgctgcg
     1741 gcgagaatat cgccctgatt cgagactcct tcgatcttga acgggtattg gcggacaatc
     1801 cgaaggtcgg aacattcatc atcgacgaag cgtctttctt cgatgagcgg ttggcgtacg
     1861 tggtgcggaa tcacagctat gaacggggag tgatgttcat cttcccttcc ctgattctga
     1921 actttcgccg cgacctcttc aacatgacgg cgcgcctgat gctggatatc gcaaccgacg
     1981 tgattcccct taccgcctac tgcgaacacc ccgattgtct ccgcgatgcg ttttacacct
     2041 accgttacta ccaggtgggt gaaaaggagt gcccggccct gtacttcgat cccttgatca
     2101 tcgtcggagg ggacgagcac aaggacagct cggtggaacc caactatgcc gcccgctgtg
     2161 acgagcatca ctatctgccc gggaaggagt acacgtactt tatcctcaag ccgctgggcc
     2221 aaaaagcggc cgccggggac gagaaaccgc tcattaatga actcaccacg ctgaaagagg
     2281 agatcaaagc ttcccaattg tggaagaact tcaccgaacg gtatgagaag ctgaacgaca
     2341 aggagattct cttcaactcg ctggaggtgg agaaaatcgc cgagaaagca ctgctctatc
     2401 tcttcgttga acaaaacctc atcccagaag atcttcttct tcgaatcgtc gatctcctcg
     2461 agctggatgt cgcctacatg agcaaggtgc tcaccgacaa caagcgcccg gtttcgttcg
     2521 cccagccgct tctcttctga aagtactgga acgtgcaaag accccacgca atagtccatt
     2581 gaggggggtc ttttcgtgtc ttacaatgag tagaacgtcg agagcgtgct ttccaaggtt
     2641 gccgggttcg cattctccag aagagcgggg acggagatct tccgagagcg cagatcggca
     2701 agcagcgccg gccagtcgaa gacgccttca agagcgggga ggtttccgat cttgaagccg
     2761 tgttcgtcga ggacgaagtc cttgacgtga agggcggcga tccgcaggcc gaaggcatca
     2821 agggcgctgt cggtgaaggc gcgctgggct tcaagtgaag gccttgcgcg aaaggagccg
     2881 tcggcttcgg gaatcccgat ccagggaatc aggttgacgg gatcgtagat gatcttcagg
     2941 cggggggtgt cgaagcgctc ggcgagacgg gccatccgtt cgatcgaaca gatcgtgttc
     3001 tgtttggcaa ccgcctcgac cgccacgacg gatccgactt tgacggcgta ttcgaccagg
     3061 cggtccaagg aggagtagag cgtttcaaga tgtttgggtt cagcggtatc ggggtggtag
     3121 gagcagtcgg gccggaccga cccggtttcc gttccgacca tcggcgctcc gagaatcgaa
     3181 gcgagcgcaa ggtgctcttc aaagcgcttg agttggcgct cccgctcggc gggatcgggg
     3241 tgaatcggat tgatatagca gccgagcact gcgatggaaa catgctgcct gtcaaacgct
     3301 tccctgatcc gaagcgcgaa cgcttcatca aggggaccgg aggaaggaaa ggatttggcg
     3361 acggccagct gcaccggcag gtgttcgccg aacgttcgaa cagcggcagc gagcgcctcg
     3421 ggagtatcaa aggagcccag atcatgggca cgaacaccga atctcatcat acgggcagta
     3481 taaaaggatt tggaaaggga gtaaagaaag aagaagatgg cgacgatgcg ggcaacaacg
     3541 ttgattgacg ggggaacata taacaacacg gaacgttttc aaacactctt tgatgactta
     3601 accgaaggtg atgagctggt catcccttcc ggtgtctatg ccaccggagc gctgacgctc
     3661 aaaaagcaca acgtaacgat tacgttggaa aagggatgcc ggattcagtt catccccgac
     3721 cctgagcttt atcctcccgt atatacccga tgggaagggg tgaactgttg ggcgatgcag
     3781 cccgccttat tgattaccga cagctctcac atccgcctgc ggggtgaagg gattctcgac
     3841 ggaagcgggt cgtggtggtg gcagcaagcg gtagtaaaga aaaagcaaag caaaccgatt
     3901 accgatcttg aacactcttt cgctcacctg aatcccggct ttgaggagca ggcaagtggc
     3961 ggtggcggtc gcaaaagcca gtttctccgc ccgcctctct tgcagatcca tcagagcagg
     4021 aacgtccaca tctcagggat cactctgcaa aactccccgt tctggacgct tcatcccctc
     4081 tacagcaaac gggtaacgat tgaggacgtt acgatcttca atcccgaaga cgctcccaat
     4141 accgatggaa tcgatattga ttcatgcagg gatgtcgtag tcagaaactg catcgtcgat
     4201 gtgggggatg acggcatcgc cataaagagc ggtagcggaa gcgacggtgt cgtcaccaac
     4261 caaccgaccg ttggggtcgt cattgaagga tgcaccgttc gaagcgccca tggcggggcg
     4321 gtgatcggca gcgaaaccgc agccggggtg cgggacgttc ttgttcaagg gtgcatcttt
     4381 gacggaaccg atcgaggcat ccggatcaag acccgccgcg gccggggagg tgagatcacc
     4441 aacatccact accgatccct cacgatgaag gacaacctct gtcctatcgc ggtaaacatg
     4501 tattaccgcc cgggagccac caatcccgac ctcttcagct tggatcctca gccgatattg
     4561 gataccacgc cgtccatttc caacatcctg atcgaggaat gcaccgccgt gggggcgact
     4621 tcaagcgccg gcttcattgt cggtcttccc gaggagccga ttcgcaacgt ccggatcgtg
     4681 cgctcctcct tcggtgtttc gaatgagaac gtcacggcga tcgaggagag tgagatgtat
     4741 gatggacttc ctgccctccg cgagcgggga atccgcctca gaaacgtcca ccttcacctc
     4801 gaggatgtaa aggtcctcgg ggtgaaggaa gggtttgtcg tcgaagaggg ggttacgttc
     4861 gattcttagc ctttgatcag gtggaacgcg aagccggcga tctcttcatt gaagtagcag
     4921 ctcttcatcg ctcccttgtc atcccgcttc acggattcct cattgagggt gaatcccttc
     4981 ttttgaaggt agtggacggt tcgatcgacg ttgttgcatg caaagccgag atgtccgtgc
     5041 tttccgtaga acggcgcgtg ggtcacttca atgacggtat cgaggaacgt cgaggaattt
     5101 ccgacctttc tggtcatccc cagaagctca agcgacgctg cggcgacttg ggcgttctct
     5161 ttggttgtct cattgattcc gaggtgggcg aagcgaagac cctggagggc gagcaccgct
     5221 tccgcacaga gggttgtgat ctcgtcccac tgctcgtttt caatcagatc actcttgacc
     5281 atccacgatc cgccgaccgc aaggacgttg gaaagagcgg cgtaggaacc gagattcttg
     5341 gcgttgatgc cgccggtggg catgaacatc acgttggaga agggaccggc gaagtttttg
     5401 agcatgtcga ctccgccgct cgcttcggcg gggaagaact tgagggtgga aagaccgcgg
     5461 ctcaagcccg cttcgatatc gctcggggtg cagacacccg gaacgatcgg aacgttgttt
     5521 tcaagcgccc aatcaacgac ctcggggttg aaaccgggcg aaacaatgaa tttggctccg
     5581 gccttgaccg ctttcttggc gaactcgacg ctggttaccg ttccggctcc gaggagcatc
     5641 tcgggcaccg ctttggcgat attggagatc gcttcttccg ctgcggcggt tctaaaggtg
     5701 acttcggcta cgggcagccc tccggcgatc atcgctttgg ccaggggtac ggccttggcg
     5761 gcgtcatcaa tctttacgac ggggaccaga ccgatgagtt ggattttttt catcagagtg
     5821 tcagacatta caatgcctcc ttattaaata tgaaacagtg tttcaaaaaa cattgacagt
     5881 gtgaactata ctctctatag taggtaatag caagagataa ggaggaaaac cttgagtaaa
     5941 aaatttgtaa catttggtga gattatgctt cgcttgaaaa gtccgggcca tgaacgattt
     6001 ttccagagtc cggccttgga ggcgacgttc ggtgggggag aggcaaacgt tgccgtatcg
     6061 ctctccctgc ttggtaaaaa agctcagttt gtaaccgctt tgccgaagaa tgcgatcggg
     6121 gaagcggcgg tgagggaagt tcgacgctac ggggtggata cttcggcgat tctccgaacc
     6181 gaggaaggcc gagtgggaat ctatttcctt gagaccgggg cgaaccaacg gccgagtctc
     6241 gtcgtctatg accgcgccga aagctcgatc gcgaccgcaa agccgagcga ttttgatttt
     6301 gccaagatcc ttaaggatgc agcgtggctc cacgtcacgg gaatcactcc ggcggtcagc
     6361 gccgaggcgg cggcatcgac cctggcggca ttgaaggaag cgaagaagca gggagtcacg
     6421 gtatcgatcg atctcaacta ccgcaagaag ttgtggaaat ggggcaagac cgccgttgaa
     6481 gtgatgcgcg agcttgcaac ctacgcggat gtaatcgtcg ccaatgaaga ggacatccag
     6541 aagtgcctgg gaatcgaagc gaacgtcgat gttacttccg gtgagctgga taccaacgtc
     6601 tataaagcgc tcaccgaaga ggtgaaaaag cagttcccga acatcagcgt catcggcgtc
     6661 accctgcggg aaagccgcag cgcggatgtg aacggctgga gcgccgccat caacggaaag
     6721 accggtttct atctttcccg ttcctacgag atcaccgaca tcatcgatcg ggtcggcgga
     6781 ggagactcct tctcggccgg cttcatctac gccttgagcg agtacggaga tgacgaggag
     6841 tatgtgatca acttcgcggt cgccgcgtca gctttgaagc attcgatcag cggtgacttc
     6901 aacctgacca ccctgagcga ggtgaaagcg ctcgtcaaag gggacggatc gggtcgggtt
     6961 cagcgctaga tgttttcttg gcgtgaaaaa agagctcttc cgtggccaag gcgaccttgg
     7021 tcaccgcgaa gggcttttct ttcgggtaga ggtagtaggt atcatccagc gtcgacagat
     7081 cgatgcaatc gccttttccc ttgtattcat attcgatgtg gcagctgtac atcgaagaaa
     7141 cgtccttatg gaggaacagc ccttcggttt ccagggtgaa gcccttcata tcgtgcacga
     7201 gccggcctat cccgatggga atgtatcccc gggcattggg gagcgcttcg atcatcaccg
     7261 gcacatcaag agaataggtt cctttctcca cctcccggcg gacattgctt cgctcccact
     7321 cataccaatc ggggatatgg gaaaactcgg taaccaaggc agtttcgtct tcagcgggtt
     7381 tcgaggcgcc gagttcgccg taaattccca tctcccactc ttttttgcat gccttgcacg
     7441 cgatgcggga accgaaggaa tgcatctcgt attccgtatg acagtgggcg cattggtaga
     7501 ggatcttatg cagtccttcc gcccgttttt tgaaggtgat cgcaatcccg ttctctttct
     7561 gccggcggta ctcgtcatat tcgaaggcgc tgcggatcct ttcgttgagc tcatcgaccg
     7621 aaagtgattc cgtctcctgt gcatcggcga taagaaagta gtcgcatgcg atcgggacgc
     7681 ctcgtttttt cagattccag acgggactgt tgaggtagtg cccgtgcatg ttgagcatca
     7741 ccacgggaac acccagcagc ttgcagatct tgcccagcga atcggggagg acggcggtgg
     7801 ttccacagag ggaataccgg gcttccggat agagggcgag gacatcgccg ttgacatgca
     7861 ggacttcctt gatctgccgc acgacctgaa ggtcattggt gaattttcgc ttgcagattc
     7921 cgccggcgtt tctcaacagc cattcgcggc cgatgaagcc gtcaatcgcg accacatagt
     7981 tcgcccggtg ggggaagagg gcggcggtcg tcaccttgaa gtcgatgaac gccatatggg
     8041 tgcagagcag aagaaacggc ggtctgatgc cttccatccc tctttgcctg atcttcaggc
     8101 gccttagcca tacttcaggg tagctgagca gccaagtaag cgggcgcaga taatgacgct
     8161 gaggtatggg ttttcttttc atgtcaaaac gtttcgcctt ggcaagtttt tcaagcgggg
     8221 tcatggattt tccttctcgt acgtccgatg gtaggggcat aaccgtggga ttgcaagagg
     8281 ccgggaaaga tgatttgaca actcatggat tttcactaga ctggaaccat ggataaagac
     8341 cttcttaccg agctgtatac acttgcgaaa gcgacgttcg ccgccaacct ggacggcctc
     8401 ttctttgaag aggatttgtt tgcggtttcc ctagcaaatc gaccgaccta ttacgtgacc
     8461 ttagggaacg gggtgttggt ggccaatcgc ggcaggaagg gactgacgaa cttcctcgag
     8521 tacctcgagc acgaggagac cgaggatgag ctggagttct tcgagtacca gcaggcgatg
     8581 gattcactgt acagcgtcgt tccttttgat gaggacgacg acatgcttga gaccgatgag
     8641 acgtggcgga aggagagcgg cgttctcttc gaggatatgg cggcccccat cttcagaagc
     8701 aaggtggagt actgtcttcc cgggatcatc gatgaagaag aggcgaaaga gttcatccgg
     8761 attttcaagg cgactctcca cctcatcgcc cgcctgacgg agattaaaaa agaacatccg
     8821 aagcaccggg cgatcgcgat gtggcatgat caggtttccg acggttcaaa gaagaatgag
     8881 tatattcccc gcctgacggt cggtgaaaat gaaatacttc tcgacaagct cctctttgaa
     8941 ccgtggaact acgaaaacac cttcccggta ccggagttcg ccgatgccga acagcggcgt
     9001 tactataaat cgatgaaagc gaaaagcggg aaggttttct atatggcgac gggaatcttt
     9061 cccgctcccg tatccagtga aaaatttgat gagccgacgt tcccggtttt ctatcttctc
     9121 tatgatcccc aagcccatca gatcctcgac gtgtatatgc tcgatgacta tgagaaggat
     9181 cacaatatct tcatcgatcg ctttctagca gtggtggatc gaggcgtgaa gccccaagcg
     9241 attcactgct acggccgtcg aacattgccg ctgctcacca agatcgggcc caatatggga
     9301 gtgatggtct ttgaagggaa acgggatgcc gagctcgatg aggtgatcga cgacatgctc
     9361 catcacttcc ttcacgatgg aattgaagag gaggatgagc acgaccgtta gatcgttccg
     9421 gcaagcctgg caagaaccca atcggttcgc agggcggact cgcccgtcga agggcaggtg
     9481 ccgattccca aaagctcatc aaccaccgtc tggatgaagg gctgggcgac gtgggctggc
     9541 ggaggatcgc ctgcgatggt ttcggtgcct tctttcgttc ggataacgat cggctgattc
     9601 agatcaagga cggagtaggc aagatccccc ttttcacagt ggatcgtcgt ccaatcgtca
     9661 tcggtgtagg tgttgaagag gaacgtcccc acccccgcca ctccctgatg ggttttgaag
     9721 tgtccccaga cgatgtcttc cgcccgatac gttttgctct ggttcgctcc catcccttga
     9781 gcctcgacga tcggtccgag gaggaagtcg atcagatcga gggcgtgcga gccaacgtcg
     9841 ggaaagtgtc cgcctccgct gatttccggc cgaacccgcc atgagccgcc gtcttgagta
     9901 tcccgtttct tgatctgttg gcgcatcagc acctccactg cgcgaatctc ccccaggcgc
     9961 ttgctctcca ggatcttttt cacccggagg aacttcggga gcgccctgcg gtagtaggcg
    10021 acgaagaggg ggacgttcgc ccgggcgcac ccttgaacca tctcaaggcc gtgttgatag
    10081 ttcaggccca tcggtttttc cacatagacg ggctttcccg cccgggcgac ccgaagggca
    10141 tactccttat gcgtatcggg atgggtggcg atgtagaccg catcgacctc gggatcgccg
    10201 atcaattgat cggcatcgtc ataccagcgc gggacattgt gccgtctggc gtaatccttc
    10261 gccttctcac cggttcgccg catcaccgcg acgagctcgg atccccgggc cttctgaaag
    10321 cccggtccgc tcttcacttc cgttacatca ccgcatccga tgattcccca acgtatcgtc
    10381 ttcatgaaag ccctctcctc acagtccatc gtccggcatg gaggtccgaa tcgtcaagag
    10441 acaatcgaag tgcatgggta tagataagct tatgccggag gcaacctaca agaaattctt
    10501 gtcggttggc accccgaccc ttatgcccct gtcagccggt tcaggccgtc gaactcatct
    10561 tatgtggaat ggtcttccgt tttatccgca acatcttcga taagcttgga aaaatcaagg
    10621 gcattctttt gggtgaggac ggacctgcct gtccttgctt cgatatcttt tcgggcattt
    10681 ccggcaacgg ttcctccctc tttggcaatc tctttgttct cttccaatcc atggggattc
    10741 aaggtttgag tcaattcagt ggttgtcgct tccgcaagca tggtaagaac cagctccaac
    10801 gtggacatgt tgtctctaag attttctttc ttcaggtttt tgaaatcctt gtattggcga
    10861 gtcgtcatcc cggaccatgc tttggaaatt tcatcggtga ggatggcata ttccacacct
    10921 tcccgtacgc cgtggtcgcc ccacgcatcc gtgagctctt ttctgacctg gatcgcttga
    10981 agtctttgat tgatccattc tctggaatag ccttttcgaa gatatgtctg cagagctctg
    11041 tcaatagtac gttcaggatc gacaatctca tcgattcttt cttttcctac ctccgccagc
    11101 cacattttaa acggttcggc tttcggtga
//
LOCUS       DAOSLI010000051        10783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_6000_length_10783_cov_25.017431, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000051 DAOSLI010000000
VERSION     DAOSLI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6000_length_10783_cov_25.017431"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLF67_07365"
     CDS             <1..945
                     /locus_tag="PLF67_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE89863.1"
                     /translation="LSRNRLSAMVLLMNNPLLQGQLLAFFTALCYAQNSLAHQHIGKV
                     TGSTAAAHLRMWLALPIVLLITFAAQGFLIPANLSTQAIVLLLVSGFLGYFLTDLLLF
                     RSFVLLGSRESMVLLTISPILVAIFGYFLFDEVLNLVQILGILITVGGIVIMVLFGEK
                     TAKAESTQKRLGYILATSAALTQTLANLLARSAVIEAGAITTNLLRNLGGLAAFIIYF
                     GVIKRQIKAHAKPFLENRKLLVLLFVATLFGPVLATTSQMKAYTLAPVGIVSTITQVS
                     PIILLPVDYYILKRHVSKASFLGTVLSVSGIGLLFLAI"
     gene            1008..>1666
                     /locus_tag="PLF67_07370"
     CDS             1008..>1666
                     /locus_tag="PLF67_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89864.1"
                     /translation="MRNKRRILLFSLLVGLIHCSPLLHARVFDSFTFTISNDWHTPNI
                     GYNVDDGMSYGAHIQTDFTFGLTIKLDGVSFTDKVTTETRFDLISLGVFYPFRFALRS
                     LEVTIAPHLSFMVTGNFEFRTIQNSWHLLRRIPILRIDYLDNDTLFTVQGGGELSAFY
                     PLGQGGLDTRIGFTIAPEWVNRFEATLAYYPDFATRIGLSFRYLDLGELYPTLGDYLD
                     R"
     assembly_gap    1667..1766
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1767..2518
                     /locus_tag="PLF67_07375"
     CDS             <1767..2518
                     /locus_tag="PLF67_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89865.1"
                     /translation="YLDLGELYPTLGDYLDRYQGIEISFTHTSGPLAEFFLFYPEQGI
                     SYGSYVFNVLGFNEPKTFAGADWTYSFGFALDTRPGFLQLFEISCEGFGLDIYNTSGS
                     IKNTGGLEIARHQGGLYALSYRFELFNHPWLKPYVKPFGGIQRFVYLENLIPTIEKYL
                     PAIGMELGLSFGKEDALVFGGTVWRPRLGAIAHYLFFHDRIASLFPEVYTHPKNRLRL
                     GLLIALDIGHDMSALRVQRKRPERTLAPYLP"
     gene            2571..3842
                     /locus_tag="PLF67_07380"
     CDS             2571..3842
                     /locus_tag="PLF67_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89866.1"
                     /translation="MKRACTIIILLFLGFSGLGATTFEEVSFGTGNDWITLGLGDNWD
                     DGLSYGLHATVEMKNRLHLTLALEGYTDKLVHQERYDLAKLKITYPFLFDLDRSYVRL
                     SPIGGVLIVGNLGFQNAQNFFHRILQKDELTLAYPQAETSVHLHLGGEAAYGVRIGPT
                     LLEGIIGLEYGPGWEFDWEAGLSLRMKKGGSFLTAAWRAAYPSDRYESQNLQAGHHEG
                     LKLALFHDGGLLQSFFFMYPMTGYSYGGWMVNALAFTKKPTFDEAHIAYGIGIFLDPT
                     GLRNRSNEIIYKNASLEVRYKNGPIEDSWFQVASYLLGWRFDLHEGARALPYLKVLAG
                     IERFSLKHETRTTRDEQLHPTIAFEAGSYFGRPNQWIIGNLNYRLRVAGSIHYVLGDT
                     NRPALPLAYRKQTPRWIFQIGLGLEIRHDLT"
     gene            complement(3839..>5229)
                     /locus_tag="PLF67_07385"
     CDS             complement(3839..>5229)
                     /locus_tag="PLF67_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89867.1"
                     /translation="VNLFDGSTNLLLHEDGASFLDSALSSDGSKIVTIKVKEGNTGLV
                     LVDSESARTLVGPTGDELGDPVFIDDSTILFTAEREKTFGAYRLDLESNVVEELFQDE
                     NGIFALTVVDDLLLYETAVASGRAVRALDYAGLEGTAGELAPPRNESKAGVAPSFEIT
                     PYRDTLRMNLVLPYPFMEGNQWLPGILVHTSSLLRKHTLLALAGIDVEKEYPYGAFTY
                     QYRKGRVAAESSLYAEKDYQELSLQSRVTLLSFVSSKALTELNVYGGGRIEHGNADEF
                     SGFMNLGAGMIHQKRNGRTNDFYGLSLISSTVRLQTYFQEPVITLGIANLQGQSRLGS
                     SSVMLNASLDYFYSNHVLNEIVLPIYEFVPQYFHNQKLRLGLKLRIPLTLLDLPFLYG
                     GFTGLGLEVGAQTVLYPAGNPFFEDVGFEAKLSARYEMVSGAELKFFVAFHVLSSGAW
                     DYTIGIDGLSLF"
     assembly_gap    5230..5329
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5330..6594)
                     /locus_tag="PLF67_07390"
     CDS             complement(<5330..6594)
                     /locus_tag="PLF67_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89868.1"
                     /translation="MKRFLFVVLFLSSTLLFASSWKQIETVHTRIIFEEEEAEAAREI
                     ASFADEIFEELAALLENPTTKRVPVILAGRTAWANGMYAPFPSSIILYLASDEDQFLG
                     TKTRSWLRQVYIHELTHYLHLTKAEGIYKILRIFGPGMTMIPVSFMPGWWIEGITTYA
                     ESMDGESGRGNSLKFDLLTQKPIDEDSMWSLAKGAYSGVYPPSGRIYATGYIMIEYLI
                     RTYGFSAFNEINRTFVNFPFMGMNWIFKRVIGITAKELFEAAIEERIRTVPSSLGFLY
                     AEGKGGSAFLPARTEAGIVGVVSTPKEGTFLKEYETGKRIATLPIGKASAISLGDEYA
                     LFSTRWIDATSPNSLVLAPEGYSDLFLYRFGDGEGKRLTNKQRLLHPRLSKDGKRAVA
                     SLINGSFHDLVEVNLFDGSTNLLLHEDGA"
     gene            complement(6633..7658)
                     /locus_tag="PLF67_07395"
     CDS             complement(6633..7658)
                     /locus_tag="PLF67_07395"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOE89869.1"
                     /translation="MEKKSIDWANLSFGYLPTDWRYVSVYKYGAWDDGGLTDDPNVVM
                     SECAGALQYAQTCFEGLKVYTTVDDRIVAFRPDLNAQRMYETAKRLVMPPFPVDRFLS
                     ALDEVVTANKAFVPPYGSGASLYIRPYMYGSGAIIGVAPAPEYTFRIFTTPVGPYFKG
                     GVKPLRLLVSEYDRAAPNGTGHIKAGLNYAMSLYAGLEAKKQGFDENMYLDSQTRTYI
                     EETGGANFIFVTKDGKVVTPKSPTILPSITRRSLISVAREYLGLEVEERKIELSELPE
                     FVEGGLCGTAAVISPIGSVTTRDGEVFLPSGMKEMGPITKKLYDTLTGIQYGTIEAPK
                     GWIRTIV"
     gene            complement(7768..8313)
                     /locus_tag="PLF67_07400"
     CDS             complement(7768..8313)
                     /locus_tag="PLF67_07400"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOE89870.1"
                     /translation="MNIYDFSVNGMDGKKVDLSTFSGKALLIVNTATRCGFTPQYTEL
                     EKLYESYRDRGFEILDFPSNQFREQAPEDIDEIDRICRVDYGTEFPRFDKIEVNGAGE
                     DPLYTFLKAEKSFAGFDPSHALGKKIASIVKEADPDYEKNSDIKWNFTKFLIDRKGNV
                     VRRFEPTQSFDDISKAIEAVL"
     gene            complement(8417..9664)
                     /locus_tag="PLF67_07405"
     CDS             complement(8417..9664)
                     /locus_tag="PLF67_07405"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOE89871.1"
                     /translation="MITGNVHDIHWILKDRRSQGPELAKKFFAKGYAQNAHRFHQQLP
                     GYQFTDLVGLPKLSELLGVGGIWTKDESQRLGLNSFKSLGGSFALYRYLQKQLGILGE
                     DTPFSYLASKEVKEKVGDLVFASATDGNHGRGVAWAATQLGFSSIIYVHKDTSIRRIE
                     AIESAGAKVVVIDGTYDDAVRQVALDSDKNGWVVISDTSWDGYTEVPTWIMQGYLTLL
                     TEAQEQFGKAGIAKPTHVIVQAGVGALAAAVIGHYHALFGDDAPLCYVLEPEKAQCLY
                     QSALADDGKPRSVGGDLDTLMAGLACGDPSPLAWEIIDAYADGFIVIPDELAAKGMRI
                     YASPIPGDPQVKSGESGAGGLGLLATIMGNSAYAGLREQLGLDETSQVLFISSEGDTD
                     PVHYKEIVWEGSSPVSKQYYNEY"
     gene            complement(9755..>10783)
                     /locus_tag="PLF67_07410"
     CDS             complement(9755..>10783)
                     /locus_tag="PLF67_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOE89872.1"
                     /translation="MKRVPRILLFLLLSVILLVSCKPKPVDPKSQTQLLLGTVCTITL
                     YDKSDNTEFDLAFDRIDEIERLMSSHLDDSELARVNQASGTHPVVVSGETFAVIKLAL
                     EIAELSGGAFDPTIGPLVKLWDISGANNVPGDGEIQSLLPLVDYKLVVLDEGASSVYL
                     PIQGMALDLGGIAKGWAADEAGRVLSGHKGIVNLGGNVLVLDSKPDESPWRIGIQDPD
                     TSRGSYIGIVDLIDRTLVTSGPYERFFEHEGTIYHHILDTKTGYPVASPLTSVSIITD
                     RSVIADGLSTAVYALGLEKGLELIDRLDGVEAIFLTKDRSVYASEGILDGSIAFKITH
                     PEYTIVSR"
BASE COUNT         2313 a         3069 c         2696 g         2505 t
ORIGIN      
        1 ttgagcagaa atcgtctctc tgctatggtt ctccttatga acaaccccct cctgcaaggt
       61 caactgttgg cgttcttcac agcgctctgc tatgcccaaa actcccttgc ccaccagcat
      121 atcggaaaag taaccggctc caccgcagcc gctcatttgc ggatgtggct cgcccttccg
      181 attgtcttgc tcatcacctt cgccgctcag ggttttctca taccggcaaa cctttcaacc
      241 caagcgatcg tccttctcct cgtcagcggc ttcttgggat attttctgac cgatcttctg
      301 ctctttcgtt ccttcgtgtt attgggatcc cgggagtcga tggtgctttt aacgatctcc
      361 ccgatcctcg ttgcgatctt cggatatttc ctcttcgatg aggttctcaa cctggtccag
      421 attctcggaa ttctcatcac tgtcggggga atcgtcatca tggttctctt cggcgagaag
      481 accgccaaag cggaaagcac ccaaaagcgt ctgggataca tccttgccac ctcggcggcg
      541 ttgactcaga cccttgccaa tcttcttgcc cgttcggcgg tcatcgaggc gggggcaatc
      601 actacgaacc tattgagaaa cctcggagga ttggcagcgt tcatcatcta tttcggagtg
      661 atcaaacgcc aaatcaaagc ccatgccaag ccctttctcg aaaaccgaaa gctgcttgtc
      721 ctcctcttcg tagcgaccct cttcggtccg gtattggcga cgaccagtca gatgaaagcg
      781 tataccctcg ctccggtggg aatcgtttca acgatcactc aggtctcccc aatcatcctc
      841 cttccggtcg attattacat tctcaaacgc cacgtttcca aagcttcctt tttgggaact
      901 gttctctctg tttcagggat tggattgctc tttttagcaa tctgagcacc cgtttccctt
      961 gagcttggcg acctctttgt ttcacaatga gccgtacggg aacggctatg aggaacaaac
     1021 gaaggattct gttattctct cttcttgtcg gactgattca ctgctccccg cttctccacg
     1081 ccagggtttt tgacagcttt acctttacca tctccaacga ttggcatacc cccaacatcg
     1141 gatacaacgt tgatgacggg atgagctacg gggctcatat ccaaaccgat ttcaccttcg
     1201 gactgactat aaagctggac ggggtttcct tcaccgacaa agtcaccacc gaaacccgat
     1261 tcgaccttat ctccctcggt gtgttctatc cattccgatt cgcccttcgt tccctcgaag
     1321 tgacgatagc gccccatctg tccttcatgg tcaccggaaa ttttgaattc agaacgattc
     1381 aaaacagctg gcatctcctt cgcagaatcc cgattctccg aatcgattac ctcgataatg
     1441 acaccctctt cacggtccag ggcggagggg agctttcggc attttatccc cttggtcaag
     1501 gtggtctgga tacccgaatc ggcttcacga tcgccccgga gtgggtgaac cgcttcgaag
     1561 cgacgctcgc ctactaccct gatttcgcga cccgaatcgg tctttctttc cggtatcttg
     1621 atcttggtga actctatccg acgctgggtg attacctgga tcgctannnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnggta tcttgatctt ggtgaactct atccgacgct
     1801 gggtgattac ctggatcgct atcaaggaat tgaaatctct tttacccaca caagcggacc
     1861 gcttgccgag tttttcctct tctaccccga acagggcatc tcctacggtt cctatgtctt
     1921 caatgtgctc gggttcaacg aaccaaagac ctttgccggt gccgactgga cctactcctt
     1981 cgggtttgcc ctcgacaccc gcccgggctt ccttcagctc ttcgaaatct cctgcgaagg
     2041 gttcggattg gacatttaca acaccagcgg ctcgatcaaa aacaccggcg gcttggaaat
     2101 cgcccgtcac cagggcggac tctatgccct cagctatcgc tttgagctct tcaaccaccc
     2161 ttggctcaaa ccatatgtaa aaccctttgg aggcatccag cgcttcgtct accttgaaaa
     2221 cctcatcccc acaattgaga aatatcttcc ggcgatcggt atggagcttg gtctcagctt
     2281 cggaaaggag gacgctctcg tcttcggcgg aacggtgtgg cggcctcgtc tcggcgccat
     2341 cgcccactac ctctttttcc acgaccggat tgcgtccctc tttccggagg tctacaccca
     2401 tcctaaaaac aggctccggc ttggcctgct catagcactg gacatcggac acgacatgag
     2461 cgccctcaga gttcaaagaa aacgtcctga gaggacgctt gccccctatt tgccgtaaaa
     2521 cacctctctc tttgctgtac cgggaaacaa tcttttgtac aatcaaggtg atgaaacgag
     2581 catgcacgat cataatcctt ctctttctcg gtttttccgg cttgggggcg acaacgtttg
     2641 aagaagtgag cttcggcacc ggaaacgact ggatcaccct tggcctcgga gacaactggg
     2701 atgacgggct cagttacggc ttgcatgcaa cggtggagat gaaaaaccgg cttcacctta
     2761 ccttggccct tgaagggtat accgacaagc tcgtccatca ggaacgctat gatcttgcca
     2821 aactgaagat cacctatccc ttcctctttg atctagaccg ctcctatgtc cggctctctc
     2881 cgattggcgg agtgctcatc gtaggaaatc tggggttcca gaacgctcag aacttttttc
     2941 atcgaatcct gcaaaaggat gaactcaccc ttgcctatcc ccaggcggag acctcggtcc
     3001 atcttcatct cggaggagaa gccgcctatg gcgtacgaat cgggccgaca ctgcttgaag
     3061 gaatcattgg tctcgaatat ggcccgggat gggagttcga ttgggaggcg gggctcagct
     3121 tgcggatgaa gaagggtgga agctttctca ccgcagcgtg gcgggcagcg tatccttccg
     3181 accgatatga aagccaaaac ctccaagccg gccaccatga gggcttgaaa ctcgccctct
     3241 tccacgatgg aggtctcctc cagtcctttt tcttcatgta tccgatgacc gggtattcct
     3301 acggcggatg gatggtcaac gcccttgcgt ttactaaaaa gccgaccttc gacgaagccc
     3361 atatcgccta cggaatcgga atctttctcg atccgacggg cttgagaaat cgatcgaatg
     3421 agatcatcta caagaatgca agtttggaag tgcgctacaa gaacggtccg atcgaagatt
     3481 cctggttcca ggtcgcttcc tacctcctcg gctggcgctt cgacctccat gagggtgctc
     3541 gggctcttcc atatctcaaa gtcctggcgg gaatcgagcg cttcagcctc aaacacgaga
     3601 cccggacgac ccgggacgag cagcttcatc cgacgatcgc cttcgaagcg ggctcgtatt
     3661 tcggcagacc gaatcaatgg attatcggca acctcaacta tcgtcttcga gtagcgggaa
     3721 gcatccacta cgtgctggga gatacgaacc gccccgccct cccccttgca tatcggaaac
     3781 aaacaccccg gtggatcttc cagatcggcc ttggccttga gatccggcac gacctcactt
     3841 agaagaggga aagaccgtcg ataccgatcg tatagtccca tgctccgctg ctgagcacgt
     3901 ggaatgcaac gaagaacttc agctcggctc cgcttaccat ctcataccgg gcagagagct
     3961 tcgcttcgaa accgacatct tcaaagaatg gattcccagc gggatacaac accgtctgag
     4021 cccccacttc aagaccgagg ccggtaaaac ccccatagag gaagggaaga tcgagaagcg
     4081 taaggggaat tcggagcttc aacccaagcc gaagcttttg gttgtgaaag tattgcggta
     4141 cgaattcata gatcggcaga acgatttcat tcagaacatg gtttgaataa aagtaatcaa
     4201 gcgaagcatt gagcatcacg ctcgatgaac cgagtcgact ctgtccttgg aggttcgcaa
     4261 ttcccagcgt gatcaccggc tcctggaaat aggtctggag tcgaaccgtc gaggagatga
     4321 gggaaagacc atagaagtcg tttgttcggc cgtttcgctt ttggtggatc atccccgctc
     4381 cgaggttcat gaagccgctg aactcatcag cgttcccgtg ctctattctg cctcccccgt
     4441 atacgttcag ttcggtaagc gcttttgaag aaacaaagga gagcagggtc actctgcttt
     4501 gaagggagag ctcctgatag tccttttcag cgtagaggct gctttccgcc gccacccttc
     4561 ccttccggta ttggtaggtg aatgcaccat acggatattc tttttccacg tcgattcccg
     4621 ccaaggcgag cagagtgtgc ttgcgcagca agctggaggt atgaacaagt atcccgggga
     4681 gccattgatt tccttccatg aagggatagg gcaagaccaa gttcatgcgc aatgtatcgc
     4741 ggtagggagt tatttcaaac gaaggagcaa cgcctgcctt ggattcattt cggggtggcg
     4801 ccaactcgcc cgccgttccc tcaagacctg cgtagtcaag agccctgacc gctcggcccg
     4861 aagccaccgc cgtttcatag aggagcagat cgtccaccac cgtaagagcg aagattccgt
     4921 tttcatcctg aaagagttct tcaacgacgt tgctctcaag atcgaggcgg taggcaccga
     4981 aggtcttctc ccgctcagcg gtgaagagga tcgtcgaatc atcaatgaaa accggatcgc
     5041 caagctcatc gccggtcggt ccgacgagag ttcgagcact ctcgctatca accagcacca
     5101 gacccgtgtt gccttctttg acctttatcg tgacgatctt cgatccatcg ctgctcaggg
     5161 cgctgtcaag gaagcttgcc ccatcttcat gcagcaggag gtttgtcgat ccatcgaaaa
     5221 gattcacctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttgccccatc
     5341 ttcatgcagc aggaggtttg tcgatccatc gaaaagattc acctcaacca gatcatggaa
     5401 ggagccgttt atcaaacttg cgaccgcccg cttgccgtct ttgctgagac ggggatgaag
     5461 caggcgctgc ttgttcgtga gccgcttgcc ctccccgtct ccgaatcggt agaggaacag
     5521 atcactgtac ccttcagggg cgagaacaag cgagttgggg cttgtggcgt cgatccagcg
     5581 ggtactgaac agggcatatt catcaccaag cgagatcgcc gaagctttcc cgatcggcag
     5641 ggtcgctatc ctctttcccg tttcatactc cttcaggaag gttccctctt tcggcgtcga
     5701 cacgacgccc acgattcccg cttccgtccg agcggggaga aacgcgcttc ctcctttgcc
     5761 ttccgcatac aggaacccga gggaagaagg caccgttcgg atccgctcct cgatcgccgc
     5821 ttcaaagagt tccttggcgg tgattccgat gacccttttg aagatccagt tcatacccat
     5881 gaaggggaag ttgacgaagg tgcggttgat ctcattgaac gcggaaaagc cgtaggtgcg
     5941 gatcaggtat tcgatcatga tgtatccggt cgcatagatc cggccggaag gcggatagac
     6001 tccgctgtaa gcgcctttgg ccaaggacca catcgaatcc tcatcgatcg gtttttgggt
     6061 aagcaggtcg aacttcaggg agtttcccct cccgctctcg ccgtccatcg attcggcata
     6121 cgtggtgatc ccttcgatcc accatcccgg catgaaggat acgggaatca tcgtcatccc
     6181 cgggccgaag atgcgcaaga ttttatagat gccttcagcc ttggtgaggt gaagatagtg
     6241 ggtaagctca tggatataga cctgtctcag ccaggagcgg gtcttggttc ccagaaactg
     6301 gtcttcgtca ctggcgaggt agaggatgat cgaggaggga aacggggcgt acatcccgtt
     6361 ggcccaggcg gtgcgcccgg cgagaatcac cggcacccgc ttggttgtcg ggttttccag
     6421 cagcgcggcg agctcttcga agatctcatc ggcgaaggag gcgatctccc tggccgcttc
     6481 ggcttcctct tcttcaaaga tgatcctcgt atggaccgtc tcgatctgct tccaagagga
     6541 ggcaaagagc agggtcgagg aaagaaacag caccacgaag agaaagcgct tcatggtgct
     6601 gactataccc gctcttcgaa cgcttgtaca gattagacga tcgttcggat ccagcctttg
     6661 ggcgcttcga tcgtcccgta ttggattccg gtaagggtat cgtagagttt cttggttatc
     6721 ggtcccatct ccttcatacc gctgggaagg aagacctccc cgtccctagt ggtgaccgat
     6781 ccgatcggac tgatcaccgc ggcggtcccg cacaggcctc cttcaacgaa ctcgggaagc
     6841 tcgctcagtt cgatcttccg ctcttcgacc tcgagcccga gatactcgcg ggccaccgag
     6901 atcagggagc gcctggtgat cgaggggagg atcgtcggac tcttgggggt gacgaccttt
     6961 ccgtctttcg taacgaagat gaagttcgcc cctccggttt cttcgatata cgtccgggtc
     7021 tgagaatcaa ggtacatgtt ttcatcaaac ccctgctttt tcgcttccaa gccggcatag
     7081 aggctcatcg cgtagttcaa ccccgccttg atatggcccg tcccgttcgg ggcggcccgg
     7141 tcatactcgg aaacaaggag ccggagcggt ttcacgccgc ctttgaaata ggggccgacg
     7201 ggggtggtaa aaattctaaa cgtatactcg ggagccggag cgacgccgat gatcgctccc
     7261 gatccgtaca tatacggccg gatatacaga ctggcgcctg aaccgtaggg cgggacgaac
     7321 gccttgttcg ccgtcaccac ctcatcgaga gcggaaagga agcggtcgac ggggaacgga
     7381 ggcataacga gccgcttggc ggtttcatac atccgctggg cattgagatc ggggcggaac
     7441 gcgacgatcc ggtcatcgac ggtcgtgtag accttcagcc cttcaaagca ggtctgggcg
     7501 tactgcagcg ctccggcgca ctcgctcatg acgacgttgg gatcatccgt caatcctccg
     7561 tcatcccatg caccatattt gtacaccgaa acataccgcc aatcggtagg aagatacccg
     7621 aagctcaaat tcgcccaatc tatcgacttt ttctccacgc tcatgccccc aaaaagatat
     7681 tgagaccatt gtatcgagct tcatgcactt cgtctataat tatgcaatat tattgtatat
     7741 agcgtgaaaa aagaaatcat cgcttcccta gagaaccgcc tcaatcgcct tggagatgtc
     7801 gtcaaagctt tgggtcggct cgaagcgccg gacgacgttt cccttccggt cgatcagaaa
     7861 cttggtgaaa ttccacttga tgtcgctgtt cttctcatag tcgggatccg cctccttcac
     7921 gatggaagcg atcttcttgc cgagggcatg ggaaggatcg aagccggcaa agctcttctc
     7981 tgctttcagg aacgtgtaca gcggatcctc ccccgccccg ttgacttcga tcttgtcaaa
     8041 ccgagggaac tccgtcccat agtccactcg acagatccga tcgatctcat cgatatcctc
     8101 gggcgcctgc tctctgaact ggttgctcgg gaaatcgagg atttcaaacc ctctgtctct
     8161 atagctctca tagagcttct ccagctcggt gtattgcggg gtgaagccgc agcgggtagc
     8221 ggtgttcacg atcaggagcg ccttgccgga aaaggtcgac aagtcgactt tttttccatc
     8281 catcccgttc acggaaaaat catatatgtt catgtgttgt ctcctattat attgccttat
     8341 attatatttt gcacaatata tatgggaata ataaagctcc tcacctgtcc cgtcaagaga
     8401 tgtgaggagc caaatcttag tattcgttgt aatactgttt actgaccggt gaagagcctt
     8461 cccaaacgat ttctttgtaa tggaccgggt cggtgtcccc ttcactggag atgaagagca
     8521 cctgacttgt ttcatcaagt cctaattgct cgcgcagtcc ggcataggcg ctgttgccca
     8581 tgatcgtggc cagaagaccc aatcctccgg ctccgctttc tccgctcttc acttgaggat
     8641 caccgggaat cggcgaagca tagatccgca tccccttcgc tgcaagctca tcgggtatga
     8701 cgatgaagcc gtcggcatag gcatcgatga tctcccatgc cagaggagaa ggatcgccgc
     8761 aggcgaggcc ggccatcagg gtatcaagat cgccgccgac gctgcggggc ttgccgtcgt
     8821 cggccaatgc gctttgatag aggcactgcg ctttttccgg ctccagcaca tagcaaagcg
     8881 gagcgtcatc cccgaagagc gcatggtagt gaccgatgac cgctgcggcc agcgccccca
     8941 ctcccgcttg gacgatgaca tgggtcggct tggcgatgcc ggctttcccg aactgctcct
     9001 gagcctcggt gagcagcgtc agatatcctt gcatgatcca cgtcgggact tcggtgtacc
     9061 cgtcccagga agtatcgctg atgacgaccc agccattctt gtcggagtca agtgcgacct
     9121 ggcgaaccgc atcgtcgtac gttccgtcga tgaccacgac cttcgctccg gcgctttcaa
     9181 tcgcctcgat ccggcgaatc gaggtatcct tatgaacata aatgatgctg gaaaacccga
     9241 gctgcgtcgc cgcccatgcg acgccgcgcc cgtggtttcc gtccgtcgcg ctggcgaaga
     9301 cgaggtctcc gaccttctcc ttcacctcct tggaggccag ataggagaac ggcgtatcct
     9361 cgccgagaat ccccaactgt ttttgcaagt accgatagag ggcgaacgaa ccgccaagcg
     9421 acttgaagct gttcaacccc agccgttgcg attcatcctt tgtccaaatt cctccgactc
     9481 ccaaaagctc gctgagcttt ggcaggccca ccaagtcggt gaattgatag cccggaagct
     9541 gttggtggaa gcgatgggcg ttttgggcat accccttggc aaagaatttc tttgcaagct
     9601 ccggcccctg tgatcgcctg tctttgagaa tccaatggat atcgtgtacg ttgcccgtaa
     9661 tcataccagc cctccgtcgt gtgaaatcta ctgcttccag tatagggcag aagatgcaat
     9721 gattccagaa gaatatgcaa caaaatgcaa caatctagcg ggaaacgatc gtgtactcgg
     9781 gatgtgtgat cttaaacgct atcgatccgt caagaatccc ttcgcttgcg tagaccgatc
     9841 gatccttcgt caggaaaatc gcttcgaccc catccagccg atcgatcaac tcaagacctt
     9901 tttcaagccc aagcgcatag actgcggtcg acagcccgtc ggcgatgact gagcggtcgg
     9961 tgatgatcga aacgctggtc agcggggaag cgaccggata tccggttttc gtatcaagga
    10021 tgtggtgata gattgttcct tcatgttcaa agaaccgttc atagggaccg ctcgtcacca
    10081 gggtccggtc gatcaagtca acaataccga tgtaggagcc gcggcttgta tccggatcct
    10141 ggattccgat ccgccaaggc gactcatcgg gcttggaatc cagcaccaag acgtttcctc
    10201 cgaggttcac gatccccttg tgcccggaaa gaaccctgcc cgcctcatcc gccgcccacc
    10261 ctttggcgat tcctcccaga tccagcgcca tcccctgaat cggcagataa acgctgctcg
    10321 ccccttcatc cagaacaacc agcttgtaat cgacaagggg aaggagcgat tgaatctctc
    10381 catcccctgg cacattgttc gcacccgaga tatcccacag cttgacgagc gggccgatcg
    10441 tgggatcgaa cgcccctccg gagagctcgg cgatctcaag cgcaagcttg atgacggcaa
    10501 aggtctctcc gctcacgacg acgggatgag tcccgcttgc ctgattcacc cgggcaagct
    10561 cgctgtcatc caggtgactg ctcatcaggc gttcgatctc gtcgatccga tcgaacgcca
    10621 aatcaaattc ggtattatcg cttttatcgt acagggtgat ggtgcacacc gttcccagca
    10681 gcagttgggt ttggcttttc gggtcaaccg gcttcggttt gcaggaaacc agaaggataa
    10741 cactcaacag gagaaacagc aggattcgcg gtactcgttt cat
//
LOCUS       DAOSLI010000052        10731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_6039_length_10731_cov_23.505339, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000052 DAOSLI010000000
VERSION     DAOSLI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6039_length_10731_cov_23.505339"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLF67_07415"
     CDS             <1..225
                     /locus_tag="PLF67_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89873.1"
                     /translation="AAVGHNAAECEHHQNNKHSYLFMHETPPKHMPQYRDKRYGVSID
                     PADELTISTSKQQEHMTYIFKNHLFVTYYN"
     gene            440..664
                     /locus_tag="PLF67_07420"
     CDS             440..664
                     /locus_tag="PLF67_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOE89874.1"
                     /translation="MRNTMDELAVGQKGSVKAINAPRQLKRRLMDMGFTKGVGVEVVK
                     MAPMGDPMEVAVRGYHLCLRKAEARSIELV"
     gene            668..2632
                     /gene="feoB"
                     /locus_tag="PLF67_07425"
     CDS             668..2632
                     /gene="feoB"
                     /locus_tag="PLF67_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOE89875.1"
                     /translation="MHTIALVGNPNSGKTTLFNALTGSTAYVGNWPGVTVEKKEGLTN
                     GHKILDLPGIYSLSPYSPEEQISRRYILNEQPDLIIDVIDATNLERSLYLSTQLAELG
                     RPLLLALNMEDLLVKEGIAIDTKRLSQMFGTPVVQISAAKGTGIAELKVEIEHALSNP
                     QTPPCPLFSSSIERKLTTLIEDDYLHEIPHGRQMRWAAIKLLEGDELFLETMPPIPPG
                     LNTYLEETRTHLQELYDDDVESIIVDQRYKVAERLARESLIQKEAKEKRFTFDMIATH
                     RIWAIPLFILIMSGVFFLSVGVVGGGTTALLEGFFENLAEWVTLTGESWQVHPFLIGI
                     LSDGIIAGVGAVLTFVPQLFILFLLLSILEDCGYMARIAFIMDRQMRSMGLSGKSIIP
                     LVIGTGCTVPAIMSTRTIEHQKQRELTIIVTPFIPCGAKMPIFALMLTYFFRNKWYMA
                     PLMYVMGILAVIVTGLLARAIDRHKETNAFILELPRYQMPSPKSVWRQTKDRTLGFIY
                     KAGTIILLASILIYLLSSYSFTFRAVDAEESMLAIMGRVIAPIFIPLGFGFWQAAVAL
                     LTGIAAKETIVSTLSVVIGTSSLTNFFDPAGALAFMTFVLLSSPCVAAMAAMLKELGS
                     KRKFLFAILWQFGFAYLVSLLVRYVVILIA"
     gene            2642..2794
                     /locus_tag="PLF67_07430"
     CDS             2642..2794
                     /locus_tag="PLF67_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89876.1"
                     /translation="MKTIIANTIVAIFILALVGFALSRIAPGKKETDDDRPSKCKDCP
                     FSGQCH"
     gene            2887..4704
                     /locus_tag="PLF67_07435"
     CDS             2887..4704
                     /locus_tag="PLF67_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89877.1"
                     /translation="MKRFWLTLLLIGVLLGSMFGATMEQKIYPVNSEPFHIITSLYIT
                     QGLALPSTAGPHSGDELMKMLDRIDADRLAPSLKNLYQRVHDELYEEQRVATWGLEAA
                     LEAYIHTDTTNFVHAEDWVRGYDERAPFLNIILETSPGKNIYGYSELPVNYTKYSDYD
                     ANTGTALSLFFGKSRFSTNLFFLPPGSIQELDLAMPYRAFGAFGGENWSVQIGRDKLS
                     WGPGKTGNFMLSDSFKYHNTGRFTTYNKNFKYSLVTSFFPHPAQYHDAIAGDDDTTIK
                     NFLKAKDRSQAQTISGLYMFLGHRLEGRLFDGKVGLALSESIMYQSEDNLLDLRFLNP
                     SMLYHNYYIRSNSNSLLTFEVDYSPIPHLNVYGQLAVDEFALPGEPVPGKDADAHPSA
                     YGFMLGVQTAIPVHKGLFTGSLEFVKTDPYLYLRYGGTGNTAQKLGEWGINYLAAIRE
                     YYAGGNIIYQEEFLGYPFGPDAIVINANAGYKEFGSWNVSTNFFYMWHGTHDKWTLWS
                     KVQPEGTAFAQGDDSKIPYIKSPTDKHWTQNQGDPNYKDRNSVAKTLIFGVKGGYTIL
                     PGFEVYAQTDLIHIVNPKNISANPAITDLQLTVGLSYSL"
     gene            4918..5721
                     /locus_tag="PLF67_07440"
     CDS             4918..5721
                     /locus_tag="PLF67_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE89878.1"
                     /translation="MKRIILVGLIVILGASLFAAGSKEQSDVDNSLDKVMKKGKFIMG
                     LDDSFPPMGFRNEKGEIVGFDVDLAKEVAKRMGVELVLQPIDWNAKEQELNTGNIDCI
                     WNGFTITPERKEAMNFSPPYVSNAQVVVVKENSPYTTLASLAGKSVGFQAGSSASDAI
                     DNSPEFKASVKNFVEFKENLTGLMDLEIGGIDALVVDLLVANDNIRRSGKPFRILEES
                     LAPESYGIGFRKNDKALADKVWEQLLSMKADGTLAKIAIKWFGADITVV"
     gene            5785..6420
                     /locus_tag="PLF67_07445"
     CDS             5785..6420
                     /locus_tag="PLF67_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOE89879.1"
                     /translation="MSNLLSLMLQGTYTSLKIFFLTLIFSLPLGLLVAKGRMSKNRFI
                     STTVNVYIMIMRGTPLILQLIFVYFAPYYLFGASYDRFTAVIVGFVINYAAYFAEIYR
                     GGIQSIEVGQYEAAHVLGFSKLHTFTHIVFPQVIKRILPSLGNEVITLIKDTALAQTI
                     GVAELFRVAQNAAARQFSTTPIFIAGVFYFVMNAIVSRSFDHIEKKLDYYH"
     gene            6431..7192
                     /locus_tag="PLF67_07450"
     CDS             6431..7192
                     /locus_tag="PLF67_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOE89880.1"
                     /translation="MEMIRVRDVKKEFGELGVLKGISFSLHEGEVLSIIGPSGSGKST
                     LLRCLTQLERIDGGSIEVCDDVMVTTDSEGNVSYSDNKTLQAIRLKLGLVFQSFNLFP
                     HMSVLRNIVEAQTHVLKRSKEEATEVARTLLAKMGLGEKEKVYPCHLSGGQQQRVSIA
                     RALALNPTVLCFDEPTSALDPELTQEILKVIKELAREKMTMIVVTHEMNFARDISNRV
                     IFMDDGLIVEEGSPQELFVNPKNQRTKRFLQRYEQ"
     gene            complement(7196..8095)
                     /locus_tag="PLF67_07455"
     CDS             complement(7196..8095)
                     /locus_tag="PLF67_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89881.1"
                     /translation="MSVKEAALKWGISERRVRVLCEEGRVDGVVRTSWAWNIPLDAPK
                     PSDGRTLRHMKNLDLRLGRMEFSRFEEQKEIIRTSDPNTLRATYHALLPKLVLGLFRL
                     EGISIEGSELAILYSQSFLPNLSFETQVIALNMRSLLKRMLPSIGLGPIRGNANPKMS
                     EQRLQLYYAQLLQGLDDDPPAYEERFIIQEGKRHPVSRQMELLFIQYEQEWRHLHPVV
                     RSLFLFGELMRIRPFGRYDWFVASLAFSEEMLKGGYLPTLIGSEEIDEFRSALLLTNS
                     KGNYQNLLALVEQQQIRAVGRLG"
     gene            8455..9780
                     /locus_tag="PLF67_07460"
     CDS             8455..9780
                     /locus_tag="PLF67_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE89882.1"
                     /translation="MAKYIESEHLELKERYTDSIAREIVSFLNASGGTILIGVKDNGE
                     VIGADRIDETLRKVSDIITNGIEPNPQDEITSELLFEEGKTIIAINITKGRRNIYCLK
                     KYGFSSAGCTMRIGTTCKEMTPEQIKIRYERNFIDTEYMLKKKSSSAGLTFRELKIYF
                     TEKGYHLDDRSFESNFHLKTEEGNYNLLAELLADRNNIPFIFVKFAGKNKATISERSD
                     YGYGCLLTTYAKIKTRLQAENICISDTTVRPRKDMYLFDFDSVNEAVINCLVHNDWTI
                     TEPQISMFNDRIEIFSHGGLPSGMTEKLFFEGVSKPRNETLMRIFLMMDLTEHTGHGI
                     PTIVDNYGKDVFEIDERYIRCTIPFNKDVVSKTKNNYNEAGKEGTLTKTEKKVLKLLV
                     KNSDMMADELADAIGVTVRTIQRAYSSLQKKGYLERIGSKKNGKWIVLK"
     gene            complement(10008..>10731)
                     /locus_tag="PLF67_07465"
     CDS             complement(10008..>10731)
                     /locus_tag="PLF67_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE89883.1"
                     /translation="GIRNIRPTIIVCPGGGYTFRSERERDPVALHFLAMSYNVFILDY
                     SVGEDAGDLNPLLELSDALMRIREHAVKWMCDPTRVAVIGFSAGAHLAASIAVLHDHK
                     KVADVQKVVDANNRPDAVILAYPVITGKEYAHKGSFDQLSRGNAEIRTLLSLEDQVRE
                     TATPAFIWHTVTDQGVLVENSLLLAQAYRKAKVPFELHLFEEGDHGLSMCTEEVGTPH
                     AGVRPWVELATSWLNRRFMHNL"
BASE COUNT         2755 a         2832 c         2646 g         2498 t
ORIGIN      
        1 gctgctgtag gccataacgc tgccgagtgc gaacaccatc agaataacaa acacagttat
       61 ctttttatgc atgaaacgcc tccaaaacat atgcctcagt atagagataa acgatacggc
      121 gtatcaattg atccggctga cgaattgacc atttcaacct caaaacaaca agaacatatg
      181 acatacatat ttaagaatca tttatttgta acttattata attaacacaa tccctttaca
      241 aatattatac atgtttttag tagacatatt acatactatt gacatatatt ttggatgccg
      301 atccgaatct caaacccaag aagacagtca ccctcttttg gaaggcaacc gatgaaaaca
      361 tgcatatctg tagcccgaaa gcagatatat cttgacagca ttgtttgttt tagctaactt
      421 atagctgagg gatacaccga tgcggaacac gatggatgaa ttggctgttg gtcaaaaagg
      481 ttcagtgaag gcgatcaacg cgcccagaca gctgaaacga cgattgatgg atatgggatt
      541 caccaaaggt gtcggtgtcg aagtggtcaa gatggccccc atgggcgatc cgatggaggt
      601 cgccgtccga ggctatcacc tctgtctcag aaaagcggaa gcacgctcga tcgagctggt
      661 ctgatctatg cacacaatcg ctctcgtagg gaatcccaac agcgggaaga ccactttgtt
      721 caacgcgttg accggttcga ccgcctatgt cgggaactgg ccgggggtta ccgttgaaaa
      781 gaaagaaggg ctgaccaatg gacacaagat cctcgatctt cccggcatct attcgctttc
      841 cccctattct cccgaagagc aaatctcccg ccgatacatt ctcaatgaac aacccgatct
      901 catcatcgat gtcatcgacg ccaccaactt ggaacgaagc ctctaccttt cgactcagct
      961 cgccgagctc ggtcgtccct tattgttggc tctcaacatg gaagaccttc tcgtcaaaga
     1021 agggatcgcc atcgatacga agcgcctcag tcaaatgttc ggaaccccgg tggtccagat
     1081 ttcagccgcc aagggaacgg gcatcgccga gttgaaagtg gagatcgagc acgctctttc
     1141 caatccgcag acgcccccct gcccgctgtt cagctcctcg atcgaacgga agctcaccac
     1201 cctcatcgaa gacgactatc ttcatgagat tccccatgga agacagatgc gctgggcggc
     1261 gatcaagctc ttggaaggcg atgaactgtt tttggagacg atgccgccga ttccgcccgg
     1321 actgaacacc tatcttgaag agacccggac gcacctgcag gagctctatg atgacgacgt
     1381 cgagtcgatc atcgtcgacc agcgctataa agtggcggaa agacttgcaa gggaaagtct
     1441 catacaaaaa gaggccaaag agaagcgctt caccttcgat atgatcgcga cccaccggat
     1501 ctgggcgatt cccctcttca tcctcatcat gagcggagtg ttcttccttt ccgtcggagt
     1561 cgtcggcggt ggaaccaccg ccctcctgga aggattcttc gaaaacctcg ccgagtgggt
     1621 aaccctcaca ggagagtcat ggcaggttca tccgttcctc atcgggattc tttccgacgg
     1681 gatcatcgcc ggagtcggag ctgtcctcac cttcgttccc cagctgttca tcctctttct
     1741 cttgctctcg attcttgaag attgcggcta catggcccgg attgcgttca tcatggaccg
     1801 ccagatgcgc tcaatgggtc tttcaggaaa atcaatcatt ccgcttgtca tcggaaccgg
     1861 ctgcaccgtc cctgcgatca tgagcacccg gacgatcgag caccagaagc agcgggagct
     1921 tacgataatc gtgactccgt tcatcccctg tggagcgaag atgccgatct tcgccttgat
     1981 gctcacctac ttcttccgga acaagtggta tatggctccc ttgatgtatg tcatggggat
     2041 tctggccgta atcgtcaccg gtctgcttgc ccgggcgatc gaccgccaca aggaaaccaa
     2101 tgcgttcatc cttgaactcc ctcgctatca gatgccttca ccgaagagcg tatggcgcca
     2161 aacgaaagac cggacactgg gcttcatcta caaagccggc acgatcatcc tgctcgcttc
     2221 gatcctcatc tacctgcttt cctcctactc gttcaccttc agggcggtcg atgcggaaga
     2281 aagcatgctt gcgatcatgg gaagagtcat cgctccgatc ttcattccgc tcggcttcgg
     2341 gttctggcag gcggcggtgg cgctgctcac cggcattgcg gcaaaagaaa cgatcgtctc
     2401 gacgctcagc gtcgtgatcg gcacttcctc gctcacgaac ttcttcgatc ccgccggggc
     2461 ccttgcgttc atgacgttcg ttctgctttc ctccccctgc gtcgcggcaa tggccgcaat
     2521 gctcaaggag ttgggcagca aacggaaatt cctctttgcc attctctggc aattcggatt
     2581 cgcctatctc gtgtccctcc ttgtgaggta cgtcgtcatc ctcatcgcat gaggtggcga
     2641 tatgaaaacg atcattgcaa ataccatcgt cgctatcttc atcctcgcac tcgtcgggtt
     2701 cgccctgagt cgaatcgccc ccggaaagaa ggaaaccgat gacgatcggc cttccaagtg
     2761 caaagactgc cccttcagcg gtcaatgcca ctgagtatct tcaaagcagc ggtcagtcgt
     2821 attcctatat accttttatg tgtgatattc tgtacaaaat atattgattg tagggggatt
     2881 tctttcatga aacgattttg gcttacgctc ttgcttattg gagtcctgct tggatcaatg
     2941 ttcggcgcta cgatggaaca gaagatctat ccggtgaact ccgaaccgtt tcacataatc
     3001 acttcgctgt atatcaccca aggcttggcc cttccttcaa cagccgggcc ccacagtggt
     3061 gatgaattga tgaaaatgct cgatcgcatc gatgcagatc gactcgctcc ttctttgaag
     3121 aacctctacc agagggtaca cgatgagctg tatgaggagc aaagagtcgc cacttggggg
     3181 ttggaagccg cactggaggc gtacatccac acggatacca ccaacttcgt ccacgcagag
     3241 gattgggtcc gcggatatga tgaacgggct ccattcttga atatcatctt ggagacgtcc
     3301 cccggcaaga acatctatgg gtacagcgag cttcccgtca actatacgaa gtattccgat
     3361 tatgatgcga ataccggaac ggctctttcg ctctttttcg gaaagtcccg gttctcaacc
     3421 aacctcttct tccttcctcc cggaagcatt caggaacttg atcttgcgat gccctatcgg
     3481 gcgttcggcg ccttcggcgg ggaaaactgg agcgtccaga tcggacgtga caagctttca
     3541 tggggtcccg gaaagaccgg aaacttcatg ctgagcgatt cgttcaagta tcacaatacg
     3601 ggaagattca ccacttacaa caagaacttc aagtacagcc tcgtcacctc gttcttcccg
     3661 catcccgccc agtatcatga tgcaattgca ggagacgatg atacgacgat taaaaacttc
     3721 ttaaaggcca aggaccgaag tcaagctcaa accatttcgg gcctctacat gttcctggga
     3781 caccggctcg agggcagact ctttgatgga aaggtaggtt tagcgctttc cgaatcgatc
     3841 atgtatcaat cggaagacaa cctcttggat ctgcgcttcc tgaacccgtc gatgctctac
     3901 cacaactact acatccggag caattcgaac tccctgctga ccttcgaagt ggattattcg
     3961 ccgatcccgc atttgaacgt ctacggtcag ctagccgtcg atgaattcgc ccttcccggc
     4021 gagccggttc cgggaaaaga tgcagatgcc cacccatccg cctacggctt catgctcggt
     4081 gtccagaccg ctatccccgt ccataaggga ctctttaccg gatcgctcga gttcgtgaaa
     4141 accgatccct acttgtactt gcgatatgga ggcacaggta acaccgctca aaagctcggt
     4201 gaatggggaa taaactatct ggcggcgatc cgcgagtact atgccggtgg aaacatcatc
     4261 taccaggaag agttcctcgg ctatcccttc ggtcccgatg cgattgtcat caatgcgaat
     4321 gccggatata aagagtttgg ttcctggaat gtctcaacca acttcttcta catgtggcac
     4381 ggcacccacg acaagtggac cctctggagc aaggttcagc cggaaggcac cgccttcgcc
     4441 caaggcgatg attcgaagat tccctacatc aaatcgccca cggacaaaca ctggacgcag
     4501 aaccagggcg atcccaacta caaggatcga aactccgttg ccaagaccct catcttcggt
     4561 gtgaaaggcg gatatacgat tctgcccggt tttgaggtat acgctcagac cgatctcatc
     4621 cacatcgtga atccgaagaa catctcggcc aacccggcga tcaccgatct tcagcttacc
     4681 gtgggcctct cctattcgct gtaagctgaa aagaccctgt tgttggtatt caacctgttc
     4741 cttgcctcaa tctcagccag gcaaggaaca ggtgttcata tcgttattca tgctttgaat
     4801 cggaatcact ttctaagaaa caatatgcac tgctttgcat aaatatgcaa aaacactgga
     4861 tattatcttc aagattcgct acactcttcc tatcatttgt ccgcaaggag tggttctatg
     4921 aaacgtatta tactggtcgg tctcatcgtc atccttggcg caagtctctt cgccgcagga
     4981 agcaaggaac aaagtgatgt tgataattcc cttgacaaag tgatgaagaa aggaaagttc
     5041 atcatgggtc tggatgacag ctttcctccg atgggtttcc ggaatgaaaa aggcgagatc
     5101 gtcggcttcg acgttgatct ggcgaaagaa gtggcaaaga ggatgggtgt cgagctggtt
     5161 cttcagccga tcgattggaa cgccaaggag caggaactga ataccggcaa catcgactgc
     5221 atctggaacg ggttcaccat cacccccgag cggaaagaag cgatgaactt ctcccctccc
     5281 tacgtttcaa acgcccaggt cgtcgtcgtg aaggagaaca gtccgtatac cacgctcgcc
     5341 tccctggccg gcaagagcgt cggatttcag gcaggatcat ccgcaagcga tgcgattgac
     5401 aactccccgg agtttaaggc ctccgtcaag aacttcgtcg agttcaaaga gaacctcacc
     5461 ggcctgatgg atcttgaaat cggcggaatc gatgcgctcg tcgtcgacct gcttgtcgcc
     5521 aacgacaata tccgccgcag cggaaagccc ttccggatcc tggaagagag ccttgctccc
     5581 gaatcctatg gaatcgggtt ccgtaagaac gacaaggccc ttgccgacaa ggtgtgggag
     5641 cagctcctct cgatgaaagc cgacggcacg cttgccaaga tcgcgattaa gtggttcgga
     5701 gccgacatca ccgtcgtatg aacaacgctg catgagcagg atacgggggc agctaccccc
     5761 gtatcgttgt gaaaggatcg tttgatgagc aatttacttt cgttgatgct gcaaggcacc
     5821 tataccagcc tgaagatctt cttcctcacc ctgatcttct cccttcccct cggcctgctg
     5881 gtcgccaagg ggaggatgag caaaaaccgc ttcatcagca cgaccgtgaa cgtatatatc
     5941 atgatcatgc ggggaactcc cctgatcctg cagctgattt tcgtctactt cgccccctac
     6001 tatcttttcg gggcctccta cgaccgcttc accgcggtca tcgtcggctt cgtcatcaac
     6061 tacgccgcct actttgccga aatctaccgc ggcggaatcc aatcgatcga agtcggccag
     6121 tatgaagcgg cccacgtatt gggcttttca aagcttcaca ccttcaccca catcgtcttt
     6181 ccgcaagtca tcaaacggat cctcccctcc ctcggcaacg aggtgatcac cctgatcaag
     6241 gacaccgccc tcgcccagac gatcggggtc gccgaactct tccgggtcgc ccagaacgcg
     6301 gcggccaggc agttctcgac gacgccgatc ttcatcgccg gagtcttcta tttcgtgatg
     6361 aatgcgatcg tctcccgctc gttcgaccat atcgagaaga agctcgatta ctaccactag
     6421 ggggcggacg atggagatga tcagggttcg ggatgtgaag aaggagttcg gcgagctcgg
     6481 ggtgctgaag ggaatcagct tctccctgca tgagggcgag gtgctctcga tcatcggacc
     6541 gtcgggaagc ggaaaatcga cgcttttgag atgtctgacc caactggagc ggatcgacgg
     6601 cggatcgatc gaggtgtgcg atgatgtgat ggtcactacc gacagcgagg ggaacgtcag
     6661 ctacagcgac aacaaaaccc ttcaggcgat ccgactgaag ctgggtctgg tgtttcagag
     6721 cttcaacctc tttccgcaca tgagcgtcct caggaatatc gtcgaagcgc aaacccacgt
     6781 tctgaagcgg agcaaagagg aggcgacgga agtggctcgg accctcctag ccaagatggg
     6841 gctcggcgag aaggagaagg tctacccatg ccacctctcc ggcggtcaac aacaacgggt
     6901 ttccatcgcc cgggcgctcg ccttgaaccc caccgtgctg tgctttgacg agccgaccag
     6961 cgccctcgat cccgagctca ctcaggagat cctcaaggtc atcaaggagc tcgcccgtga
     7021 aaagatgacg atgatcgtcg tgacccatga gatgaacttc gccagggaca tctccaaccg
     7081 ggtaatcttc atggatgacg ggctcatcgt cgaagaaggc tccccccaag aactctttgt
     7141 aaatcctaaa aaccaacgaa ctaaacggtt ccttcagcgc tacgaacagt gattattatc
     7201 caagcctgcc taccgcccgg atctgctgct gttcgacgag ggcgagaagg ttctggtagt
     7261 tgcccttgct gttggtaaga agcagcgctg agcgaaactc gtcgatctct tcactcccta
     7321 tgagggtcgg caggtaacca cctttcagca tctcctcact gaacgcaagg gaagcgacaa
     7381 accagtcata ccgtccaaag gggcggatcc gcatcagctc gccaaagaga aagagcgagc
     7441 ggaccaccgg atgaagatgg cgccattcct gctcatattg gatgaagagg agctccatct
     7501 ggcgggagac cggatggcgc ttgccttctt ggatgatgaa tcgttcctcg taggcgggag
     7561 gatcatcatc aaggccttga agaagctgag cgtagtaaag ctgaaggcgt tgttcgctca
     7621 tcttcggatt tgcatttccc cgaatcggcc ccagaccgat cgaaggaagc atccgtttaa
     7681 gaagtgagcg catattcaag gcaatcactt gtgtttcaaa gctcaggttc ggcagaaagc
     7741 tttgactgta gagaatcgcg agttccgacc cttcaatcga gatgccttca agacgaaaca
     7801 gcccgagcac cagcttggga agcagggcat gataggtcgc cctcagcgtg ttcggatcgc
     7861 tcgttcgaat gatctccttc tgctcctcaa agcgggagaa ttccatccgg cccaaccgaa
     7921 gatcgaggtt cttcatgtgc cgaagggttc gtccatcact tggcttcggg gcatcaaggg
     7981 ggatattcca cgcccaggag gttcgcacca ccccgtcgac ccgaccctct tcacagagaa
     8041 cccggaccct tcgttcactg atcccccact tcaaggcggc ctctttcacg ctcatgtaca
     8101 tcgtagtatg cttcctttat tggattgata ccatgaatgg gaatgaggga caagaatgga
     8161 ctgtaccatt atcgatgaat ggagaacact tcgcaaagga ggatccattg gaatcatccg
     8221 accttgtcat cgtcggcagc ggaacaactt cacctgcccg atcggctttc ccgaggagta
     8281 tgacatacta ctgcctatag gggaaaaacg cagtaaacat taggttttca aggcttccac
     8341 aacgtaagaa attcgaaaat cgttgtcaaa gatcaataac ccgacattat ttgtgtcggg
     8401 ttaacttgta taaatgtcgg gtataatgta ctatattgcg aggaggaata ggatatggcg
     8461 aagtatattg aatcagaaca tcttgaatta aaagaaagat acacagactc catcgcaaga
     8521 gagatagttt cttttttgaa tgcatcggga ggtaccattc tcattggtgt aaaagataac
     8581 ggtgaagtta tcggtgccga taggattgat gaaacgttga ggaaggtgtc ggatataatc
     8641 acaaacggga tagaacctaa cccgcaagat gaaataacct cagaattact ctttgaagaa
     8701 ggaaagacga ttatagccat taacattaca aagggcagaa gaaatattta ctgtctcaag
     8761 aaatacggat tttcatcggc aggttgcact atgcgaatcg gaacaacgtg caaagaaatg
     8821 acacctgaac aaattaaaat cagatacgaa aggaatttta ttgatacgga atacatgctg
     8881 aaaaagaaat caagttcggc aggtttgacg tttagggagt tgaaaatata ttttacggaa
     8941 aaaggctatc atcttgatga cagatccttt gagagcaact ttcatctaaa aacggaagaa
     9001 ggcaattata acctattggc agaactcttg gcagatagaa acaatatacc ttttattttt
     9061 gttaagttcg ccggtaaaaa caaagctaca atatctgaac gcagcgatta cggctatggc
     9121 tgtctattaa ctacctacgc taaaataaaa acgagattac aggcggagaa tatatgcata
     9181 tcagatacta ctgtcagacc gcgaaaagac atgtatcttt ttgactttga ctcagtcaat
     9241 gaggcagtga taaattgttt ggttcataac gattggacga taacagagcc tcaaatatca
     9301 atgttcaacg accggataga aatattttca catggcggtc ttccaagtgg tatgactgaa
     9361 aaactattct ttgaaggagt cagcaagcca aggaatgaga cactgatgag aatattttta
     9421 atgatggatc ttaccgagca tacaggtcac gggataccca ccattgtgga taactatgga
     9481 aaagatgttt ttgaaataga tgaaaggtat atccgttgca ccataccgtt taataaagat
     9541 gtcgtatcca aaaccaaaaa taattacaat gaagccggaa aagaaggtac gttgacaaaa
     9601 acagaaaaga aggttctgaa gcttttggtt aaaaattccg atatgatggc tgatgaactt
     9661 gcagatgcaa ttggagtcac agtaaggact atacaaaggg catattcttc attgcaaaaa
     9721 aaaggatatt tagaaagaat cggttctaaa aaaaacggaa aatggattgt cttaaaatag
     9781 gttcacattg caggtatgta acccgacacc ctccatgtcg ggttacatgt caggttaaaa
     9841 atcaacggtg aaataatgtg acgaacacag tatgagagcg cccgcagcat acgaatgaac
     9901 tatttgctct tgaatgtatg aagcaacttg cttcagtttt aaactctcca tatcaagttg
     9961 tttttccaac tctggtatgg tttcaaacct aatccgggcc caagaggtca tagattgtgc
    10021 atgaagcggc gattcagcca gctggtcgca agctcgaccc acggtctgac tccggcatgg
    10081 ggagtgccca cctcctcggt gcacatggaa agcccatgat ccccctcttc gaagaggtgc
    10141 agctcgaagg gaaccttggc ctttcggtag gcttgggcaa gaagcaggct gttctccacc
    10201 aagaccccct gatcggttac ggtatgccag atgaaggcgg gagtcgcggt ttctcgtacc
    10261 tgatcctcca gactcaacag cgtcctgatc tcggcgtttc cccggctcag ctgatcgaag
    10321 cttcctttgt gggcgtattc cttgccggtg atcaccggat acgcaaggat gaccgcatcc
    10381 ggccggttgt tggcatccac gaccttctgc acatcggcaa ccttcttgtg gtcgtgaagg
    10441 acggcgatcg aggcggccag gtgcgcaccg gcggagaacc cgatcaccgc gacccgggtg
    10501 gggtcgcaca tccacttgac ggcgtgctcg cggatccgca tcagcgcatc gctgagctcc
    10561 aggagggggt taaggtctcc ggcatcctct ccaaccgagt aatcgaggat gaagacattg
    10621 tagctcatcg ctaggaaatg aagcgctacc ggatcccgtt cccgttcact gcggaacgta
    10681 tatccgcccc cggggcagac gatgatcgtg gggcgaatgt tccggattcc t
//
LOCUS       DAOSLI010000053        10727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_6044_length_10727_cov_24.574307, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000053 DAOSLI010000000
VERSION     DAOSLI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6044_length_10727_cov_24.574307"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..778
                     /locus_tag="PLF67_07470"
     CDS             59..778
                     /locus_tag="PLF67_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89884.1"
                     /translation="MKRFLLLLMILLLSGSALFSGTGSLLLDAVVFGLDVAAKAGKGK
                     VEVTLPDEEKEISVRRVSMKKMKTTFEEHNFLLLEYEKRLEIYDRLSVPIAWPVVKGI
                     TIGFGKGSRLQRDHAGSITGIVMDTASAAAIGTGLVFIVIDAIFVGMLGGRENFSDDE
                     LKTTGENLLFGGLIALGASRVIQGIGPAVYGLRYNKILRTGLGLAKDRSDAVRPAIGF
                     APLPTGDGRLQWQVAARIPLR"
     gene            843..1565
                     /locus_tag="PLF67_07475"
     CDS             843..1565
                     /locus_tag="PLF67_07475"
                     /inference="COORDINATES: protein motif:HMM:NF017444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P13 family porin"
                     /protein_id="HOE89885.1"
                     /translation="MKRLLLLLVLLSVFAATLFAAFPGMFSLDVAIPGLAVSTSGGRG
                     EILLTLPDEGESISIGRAGLGTMKRVFDEHDFLSFEYEERLAIYDRLSVPLAWPILKN
                     ITLGFGSGSRLQRDPVGALVGVVLDSASLIVIGAGFFGMIFEGWVIGLSEGPDIDEDA
                     YMKMTSLKFLLGGVIALKASRIIQGIIPAVYGLRYNKVLRDGLGLNKDRSDALGPAIG
                     FAPIPTPDGKILWQVAARVPLH"
     gene            1622..2326
                     /locus_tag="PLF67_07480"
     CDS             1622..2326
                     /locus_tag="PLF67_07480"
                     /inference="COORDINATES: protein motif:HMM:NF017444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P13 family porin"
                     /protein_id="HOE89886.1"
                     /translation="MKRFVVLIILPVLLTSTLFAVGELLFDVAIVGLDIMVTAGKGPV
                     TVTLDEEKGPITIGGTGIKPLRRTFEEYNFSTLGYEERLRIYDRLSVPVVWPIVKNIT
                     IGFGNGSVLQYDSMGALTGAVLDGASAITLSVGLGIFIGLISVHPDTFSDQLPLFSVG
                     VDLMVAGMIGLGVSRIVQAIIPAVYGLRYNKVLREGLGLEKDRSDALRPALGFAPLPT
                     SDGRLQWQVAARVPLQ"
     gene            complement(2318..>4813)
                     /gene="dnaE"
                     /locus_tag="PLF67_07485"
     CDS             complement(2318..>4813)
                     /gene="dnaE"
                     /locus_tag="PLF67_07485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOE89887.1"
                     /translation="APHRYHALLPDRLKKRSSPYPLIALDDGFLRSEADRIVHKTLVA
                     IGTNANVGNLRVGTYCAKGRILLDQRSFEELFAPWPEAIATANSLIAYDPFPKTHIFP
                     TVTPPNGLSPIAELRRRVYLGAERRYAEVTDEVVHRIEYELSIIEAKHFSSYFLIMDE
                     IVALASRSCGRGSGAASIVSYALGITNVDPIAHNLYFERFLSPGRTDPPDIDVDFAWD
                     ERPRVFEAIKDRYGEDRIGRVANHIFYRRRNALRECARAWGQNDRAINRFIKGADSSD
                     PDWRTIRSIADHLAGLPKEIGTHSGGIIITPKPIVTYAPITRTGDGYPLVVWEKDGCE
                     LAGFVKLDILGNRSLAVIRDALSNLKADGIVIDEDRWRPIDDVETQDTLARGDSMGVF
                     YIESPAMRQLQKKTKKGDFDHIVIHSSMIRPAANRFIGEYVKRLNGKPFAHLHPRLES
                     ILGETYGILCYQEDVSKTAIALAGFDEVEADQLRKIIAKKHAHKRLVAYKEQFIRGCI
                     YNGVPIQTIERVWAMMLSFDGYSFCKPHSASYAMVSFQSAYLRTHHPAYFLAAVLTNQ
                     GGYYHGGAYVSEARRMGLIVEGPDLNRSQRAYIANGNTIIVGLMAIATLSEGAILRII
                     RERSANGLFHSLEEAASRLHLGREDWQALSQSGALEVLAPGTRRSDQLRVVLTTAYEE
                     HKDAQLELFGQANEILPVRLPPARRTLGELHAEYEALGFLRTAHPLILYATHLQRIRR
                     ILAKDLPCHIGRRVTLVGYPITMKRVFTSAREVMAFFSFEDETALFETVLFPEIYRRY
                     EGLLGSFRPLVIQGVVESDEGALTVEVDRLSSL"
     assembly_gap    4814..4913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4914..6012)
                     /locus_tag="PLF67_07490"
     CDS             complement(<4914..6012)
                     /locus_tag="PLF67_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89888.1"
                     /translation="MIIHLSIGDFSSSATIVRDPSLKAVPFVIARGSSARALVLSPSP
                     IARKEGIRVGMRLAHAQLILPSLLVIEPDYRTLLRAREAIDTVAGRYSPSVAVTKNGS
                     FFLDMRGTTRLFGPVVDSAARLRSEIHSSLGFDGSVAVASNTLVANVATQTIAPSGLA
                     SIPPGEESSFLHPQPVTLLPGVGAKIASLLHSVNIIHIGDLAGLDEKQVRSFLGPKGL
                     ELYKRAQGVMPNEGPVPTEAMIERRVRFSEPLIDYPLIKAALRSTCEESGFELRTRGL
                     AARTLSLTLQWIDGMERTRSMTLANPIVLDTQIADHAEILLQALLTRRPHVLGCTFRL
                     SRLEPEIQTIDLFDAEQEMKRQNLQAAVDRIR"
     gene            complement(6009..6779)
                     /locus_tag="PLF67_07495"
     CDS             complement(6009..6779)
                     /locus_tag="PLF67_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOE89889.1"
                     /translation="MAEILIGTSGYSYPDWVGSFYPEGSRSEEFLSLYAQSFETVELN
                     FSFYRMPQGAQLALMHQEAPKLRFSLKAHQSLTHTIDPSTYKSNASTFAQAVEPLAAE
                     GVLLAVLLQFPPSFAYTVDHRYYLDSLINILSHLPLAVEFRSPSWFNKQTLDGLKKRR
                     VSFVSVDLPQIAGTPPLLDVRTADLGYVRLHGRNKEAWWGSSDNKRYDYLYSTGELDS
                     IARRITALAEDAKRIAVYFNNHHRGQAVKNAQVLKTLL"
     gene            6870..>7597
                     /locus_tag="PLF67_07500"
     CDS             6870..>7597
                     /locus_tag="PLF67_07500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE89890.1"
                     /translation="MRGCLGIFFCDLDGTILPHGRKTVDQAFFDLVRRAQDKGYCFCI
                     SSGRYHQSLLSFFKDVENDLIFSASNGCRILHKGTDLIEPHRMEYSTVQAILADFARW
                     DVIPLLSALDGFYVHADHRDHPRVAALLGRDYTHVYKDPSEIPDTMLQMTALCRGNRD
                     ELLIKAKERYALDLHVFSTGVQLFDICPTNKGESLKEIRTLLKVREKDSWAFGDDEND
                     LSMLRSAGKGYLMNNAATHLHDQG"
     assembly_gap    7598..7697
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7698..8981
                     /locus_tag="PLF67_07505"
     CDS             <7698..8981
                     /locus_tag="PLF67_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89891.1"
                     /translation="GFSAGTKEEAVKEVYFLNFKPEIASVYETKVAPAFEAETGIKLK
                     VVTAASGTYAATLKSELAKSSPPTIFQTNGPVGLAGSLNYVSDLKNTQFYKILSDKSM
                     ALTSGDQVLAIPYAVEGYGLIYNDAIMRAYFALPNKKVSIKSAEEITSFALLKAVVED
                     MTRNKDRLGIKGVFASTSMAPGNDWRWQTHLVNVPLAYEFRDKGIPTGTKTDNFEFKY
                     ANEMKQLWDLYLDNSTTARGLLGSKSVDDAMAEFALGQAAIVQNGNWGAAQILGVQGN
                     KVADKDIKFMPIYIGVKGEEHDGLNVGTENYLCINSQVSAEKQALADQFLTWLFSSDT
                     GKAIVKNDLMFITPFNTFKDHELPTDPLAKEVIRWMNKDGVSSMPWAFSVIPSGQWKT
                     DFGADLGQYINGKMSWSDVAKRAVASWKTEYDLTK"
     gene            9044..9886
                     /locus_tag="PLF67_07510"
     CDS             9044..9886
                     /locus_tag="PLF67_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE89892.1"
                     /translation="MDKTLRRWAPLFVLPALISFIIAFLVPMIMGLVLSFFKFSTVTD
                     GVFVGWSNYKRIFTDMDFVTSLWMNVRFTVVTVISINILAFFLALLLTQGVKGTNVYR
                     TIFFMPNLIGGIVLGWIWLLIINGILLPRNATIITDAKYGFWGLVALANWQNIGYMMV
                     IYIAGIQTINRDLIEAAQIDGANSWIRLKSVILPSVMPAITICTFLTLTSGFKIFDQN
                     LALTAGNPARQTEMLALNIYNTFYTRTNFEGVGQAKAVLFTILVAIIALTQLAATRQK
                     EVEA"
     gene            9883..10725
                     /locus_tag="PLF67_07515"
     CDS             9883..10725
                     /locus_tag="PLF67_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89893.1"
                     /translation="MSIKTRTNYLHIVISILLAMLAILFVAPIIIVFMNSFKSKLFIS
                     SEPFAFPTSETFTGYANYVEGARKINFFKAFGYSLFITTFSVTAVVGTTSMLAWYITR
                     VKSLFTTFTYYLLVFSMIVPFQMVMFTMSKIANILHLDNPVGVIVLYVGFGAGLGSFM
                     FSGFIKTIPLSLEEAALIDGATPPQTFFLVVLPMLKPTAITIAILNAMWIWNDYLLPY
                     LTIGTEYKTIPVAIQYLRGGYGAVDMGAMMAMLVLAMLPIIIFYLFTQRYIIEGVVAG
                     AVKG"
BASE COUNT         2273 a         2893 c         3029 g         2332 t
ORIGIN      
        1 gaagtggata agaacaccat gatggcgtat actgataacg cacgtatcgg aggatgccat
       61 gaaacgcttt ctgttgcttc tcatgattct cttgctgtcc ggttcagcac tcttttccgg
      121 cacgggaagt ctcctgctgg atgccgttgt cttcggtctc gatgtcgctg caaaagccgg
      181 taaaggcaag gtggaggtga cgcttcccga tgaagagaaa gagatctccg tccgcagagt
      241 cagcatgaaa aagatgaaaa cgaccttcga ggagcacaac tttttgctcc ttgaatatga
      301 aaagcggttg gagatctacg atcggctgag cgttcccatc gcctggccgg tggtgaaagg
      361 catcacgatc ggattcggca agggctcccg cctgcagcgc gatcatgccg gatcgatcac
      421 gggaatcgtc atggacaccg caagcgccgc cgcgatcggc accggactgg tgttcattgt
      481 catcgatgcg atcttcgtcg gcatgctcgg gggccgagag aacttcagtg atgatgaact
      541 gaagacgacc ggtgagaatc tcctcttcgg aggtctcatc gctctgggag cgagccgggt
      601 catccagggg atcggtcccg ccgtctatgg cctgaggtac aacaagatcc tgagaaccgg
      661 actcggcctt gcgaaggatc gcagtgacgc cgtaaggcct gcgatcggat tcgctccgct
      721 tcccaccggc gacggacggc ttcagtggca ggtcgccgcc cggattcctc tccgctgaaa
      781 gagatacgta gatacgaaga ccgaaatgac gtatacttcc gacaaacttg ttgggagccg
      841 ccatgaaacg ccttcttctg cttctcgttc ttctttcggt gttcgcagca acactcttcg
      901 ccgcgtttcc gggaatgttt tcattggatg tcgccatacc cgggctcgcc gtctcgacga
      961 gcggaggcag gggagagatc ctgctcacgc ttcctgatga aggggagtcg atctccattg
     1021 gacgagccgg cttggggaca atgaaaagag tcttcgatga acatgacttc ctctcgttcg
     1081 aatatgaaga acggcttgcg atctacgacc gcttgagcgt ccccctcgcc tggccgatcc
     1141 tcaagaacat caccctcggt ttcggttcgg gctcccggct gcaacgtgat ccggtcggag
     1201 cgctcgttgg agtcgttctg gacagcgcga gcctgatcgt gatcggcgcc gggtttttcg
     1261 gcatgatctt cgaagggtgg gtgatcggtc tcagcgaggg tcccgacatt gacgaagatg
     1321 cgtatatgaa gatgacgagc ctgaaatttc tgcttggcgg cgtgatcgcc ctgaaagcga
     1381 gccggatcat ccagggaatc ataccggcag tgtatggcct gcgctacaac aaggttctca
     1441 gggatggact cggacttaat aaagatcgca gcgacgccct cgggcctgcg atcgggttcg
     1501 ccccgattcc cacccctgat ggcaagatcc tgtggcaggt cgccgcccgg gttcccctgc
     1561 actgaagtag aaaagaagac cggaatcgca tatactgccg acacattcac cggaggtcgc
     1621 catgaaacgc tttgtcgtgt tgattatcct tcctgtactg ctcacatcga ccctttttgc
     1681 cgtcggagaa ctcctgttcg atgtcgccat cgtggggctc gacatcatgg tgaccgccgg
     1741 caaaggtccg gttacggtca ccctcgatga ggaaaagggg ccgatcacga tcggcggaac
     1801 cggaatcaag ccgctgagaa gaaccttcga ggagtacaat ttttcgaccc tcggatacga
     1861 ggaacggctt cggatctatg atcgcctgag cgtccccgtc gtctggccga tcgtgaagaa
     1921 catcacgatc ggattcggca acggttcggt tctccagtac gattcgatgg gagcgctcac
     1981 cggagccgtc ctggacgggg cgagcgcgat cacgctgagc gtcgggctgg ggatcttcat
     2041 cggcctcatc agcgtccatc cggacacctt ctccgaccag cttccgctct tttcagtcgg
     2101 cgtcgacctg atggtcgccg gcatgatcgg cctcggagtg agccggatcg ttcaggcgat
     2161 cattcctgcg gtctacggcc tgaggtacaa caaggtcctc agggaggggc tcggccttga
     2221 gaaggatcgg agcgacgccc ttcggcccgc actcgggttc gctccgcttc ccacctctga
     2281 cggccggctt cagtggcagg tcgccgcccg ggtaccccta cagtgaggaa agacgatcga
     2341 cttcgacggt aagagctccc tcatcgctct cgacgactcc ttggatgaca agaggacgga
     2401 aggagcccag tagcccttca tatcgccgat agatctcagg gaaaagcacc gtctcaaaca
     2461 atgccgtttc atcctcgaag gagaagaacg ccatcacctc tcttgcggag gtgaagaccc
     2521 tcttcatcgt gatcgggtat ccgacgaggg tcacccgcct gcctatatgg cagggaagat
     2581 ccttcgccag aatccgtctg attctttgta gatgggtggc gtaaaggatg aggggatggg
     2641 cggttcgaag aaaccccagg gcttcatatt cggcatgaag ctcgccgaga gtgcggcggg
     2701 cggggggaag ccgaaccggc agaatctcgt tcgcttgtcc aaaaagctca agctgagcat
     2761 ccttgtgctc ctcgtatgcg gtcgtcagca cgacccgcag ctgatcgctt cggcgggttc
     2821 cgggggcgag tacctccaag gctccgcttt gacttaaggc ctgccagtcc tcccggccga
     2881 ggtgcaagcg ggaggccgcc tcttccaacg agtgaaacaa gccgttggcg gagcgttccc
     2941 ggatgatccg aaggattgcc ccttcactca aagtcgctat cgccatcaaa ccgacgatga
     3001 tcgtattccc gtttgcgata tatgcccgtt ggctccggtt gagatccggg ccttcgacga
     3061 tgagacccat ccgtcttgct tcggagacat aggctccccc gtggtaatac ccgccctggt
     3121 tggtcagcac cgcagcgagg aaatatgcgg gatgatgggt tctgagatat gcgctttgaa
     3181 aggagaccat cgcgtacgaa gcgctgtgcg gcttgcagaa ggagtagccg tcgaagctca
     3241 gcatcatcgc ccagacccgt tcgatcgttt gaatcggaac cccgttgtat atgcatcccc
     3301 ggatgaattg ttccttgtat gcaacaaggc gtttatgagc gtgtttcttg gcgatgatct
     3361 tacgcagctg atccgcttcg acctcatcaa aacccgccaa ggcgatcgct gtcttcgaga
     3421 catcctcctg ataacagagg attccgtagg tttctccgag aatcgattca agacgcgggt
     3481 gaagatgggc aaacggcttg ccgttcaggc gtttgacata ttcgccgatg aagcgattgg
     3541 cggcggggcg gatcatcgac gaatggatga cgatgtgatc gaaatcgcct tttttcgtct
     3601 tcttctggag ctggcgcatc gccgggcttt cgatgtagaa cacccccatc gaatcgcccc
     3661 gggcgagggt gtcctgcgtt tcgacatcat cgatcggccg ccatcggtct tcatcgatga
     3721 cgattccatc cgccttgaga ttggacaggg cgtcccggat caccgcaagg gagcggttgc
     3781 ccaaaatgtc cagcttcacg aaccccgcaa gctcgcatcc gtccttctcc cacaccacca
     3841 gcggataccc gtcgccggta cgggtgatcg gggcgtatgt gacgatcggc ttcggcgtga
     3901 tgatgattcc gccggagtgg gttccgatct ctttcggaag acctgcgaga tgatcggcga
     3961 tggaccggat cgtccgccaa tcgggatcgg acgaatcggc ccctttgata aaccggttga
     4021 ttgcccggtc attttgtccc catgcccggg cgcactcccg gagggcgttt cgccttcggt
     4081 aaaagatatg gttggccacc cgacctatcc ggtcctctcc atatcgatct tttatcgctt
     4141 caaagacccg aggccgctca tcccaggcga aatcgacgtc gatatccggc ggatcggttc
     4201 ggccggggga gagaaagcgt tcgaagtaga ggttgtgggc gatcggatcg acgtttgtga
     4261 tccctagggc gtaggagacg atggaggcgg cgccgctgcc gcgcccgcag gaccgggagg
     4321 cgagggcgac gatctcgtcc atgatgagaa agtaggaaga aaagtgtttt gcttcgatga
     4381 tggaaagttc gtattcgatg cggtggacca cctcgtcggt aacttcagcg taccgtcttt
     4441 ccgcgccgag gtagacgcgc cttcggagtt cggcgatcgg ggacagcccg ttaggcggcg
     4501 ttaccgtcgg aaagatatgg gtcttcggaa acgggtcgta ggcgatcagg gagtttgcag
     4561 tggcgatcgc ttccggccac ggagcgaaga gctcttcaaa tgaacgttga tcgagcagaa
     4621 tccttccttt tgcacagtag gttccgacac gaagattgcc gacgtttgcg ttcgttccga
     4681 tcgcgaccag cgtcttatgc acgatccgat ccgcttcact tctcaggaat ccgtcgtcga
     4741 gggcgataag cgggtagggc gatgaacgct tcttgagtcg gtcgggcagc agtgcatgat
     4801 accgatgcgg ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcggat
     4921 ccggtcgact gcagcttgga ggttttgccg cttcatctct tgttcagcgt caaaaaggtc
     4981 gatcgtctga atttccggct caagacgtga aagtctgaag gtgcatccaa gcacatgagg
     5041 ccgccgggtc agaagagcct gcaagaggat ttccgcatga tctgcaattt gggtgtcaag
     5101 aacgatcggg ttggcaaggg tcatcgaccg cgtccgctcc attccgtcga tccactgaag
     5161 agtaagcgaa agggttcggg cggcgaggcc ccgagtccgc aattcaaaac cgctttcttc
     5221 gcaggtcgag cgaagcgcgg ctttgatcag ggggtagtcg atcagcggct cactgaagcg
     5281 gacccggcgt tcgatcatcg cttcggtcgg gaccggtccc tcgtttggca tgacgccctg
     5341 tgcccgtttg tacagttcga ggcctttcgg ccctaagaac gaacggacct gtttctcatc
     5401 caagccggcc agatcgccga tgtggatgat gtttaccgaa tgaagaagcg aagcgatctt
     5461 cgctccgaca cccggcagaa gggtgaccgg ttgcgggtgc aaaaaggagg actcctctcc
     5521 cggggggatc gaggcgagcc ccgagggggc gatcgtctgc gtggcgacgt tcgcaaccag
     5581 agtgttggaa gcgacggcga cgcttccgtc aaaacccaag gaggagtgaa tctcgcttcg
     5641 cagccgtgca gcgctgtcga ccaccggtcc gaagaggcgg gtcgtccccc tcatgtccag
     5701 gaagaaggat ccgtttttcg tcaccgccac gctcggacta tagcggccgg cgactgtatc
     5761 aatcgcttcc cgagcgcgaa gaagggtgcg gtaatccggc tcgatgacca gcagggaggg
     5821 aaggatcagt tgggcgtgag caaggcgcat gccaacgcgg attccttcct ttctggcgat
     5881 cggagagggg ctgaggacca acgcccgggc ggacgatccc cgggcgatga cgaacggaac
     5941 ggctttcaag gaaggatccc tgacgatcgt tgcgctcgag ctgaaatcgc cgatcgaaag
     6001 atggatgatc ataggagggt cttaagcacc tgggcgtttt taaccgcctg cccgcggtgg
     6061 tggttgttga aatacactgc aatccgcttg gcatcttccg ccaacgcggt gatccgtcgg
     6121 gcgatcgaat caagctcgcc ggtactgtag agatagtcat agcgcttgtt gtcggaagac
     6181 ccccaccacg cctccttgtt tcggccatgg agccggacat accccaggtc ggcggtccgg
     6241 acgtcgagga ggggcggagt gccggcgatc tgaggcagat cgaccgagac gaagctcacc
     6301 cttcgtttct tcaatccgtc gagggtctgt ttgttgaacc aggaggggga acgaaactcg
     6361 accgccagcg gaagatggct aagtatgtta atcaaggaat cgaggtaata gcgatgatcg
     6421 acggtgtagg cgaacgacgg aggaaattga agaaggaccg caaggagcac gccttcggcc
     6481 gcaagcggct cgacggcttg ggcaaacgtt gaagcattgc tcttgtatgt gctcggatcg
     6541 attgtatgcg tgagactttg gtgagctttc aaggagaagc ggagcttcgg ggcctcttgg
     6601 tgcatgaggg caagttgggc gccttgcggc atccgataga aggagaagtt cagttcaacg
     6661 gtttcaaagc tttgggcata gagggagagg aactcctcgc tcctgctgcc ttccggatag
     6721 aaggatccaa cccaatcggg atagctgtag ccggatgtac cgataagaat ctccgccata
     6781 cgtcacagat actatatgtt tgttaagtag tcaagagttg ttgtcaatcc tttgcatctc
     6841 attcatactg atgactgacg gagggctttg tgcgcgggtg tttaggcatc tttttttgtg
     6901 atcttgacgg gacgattctt ccccatggac ggaaaaccgt cgatcaagca ttcttcgatc
     6961 tggttcgccg ggctcaggac aaagggtatt gtttctgcat ctcaagcggc aggtatcatc
     7021 agtccctcct gtccttcttc aaggatgtgg aaaatgatct catcttctcc gcctcgaacg
     7081 gctgtcggat tctgcacaaa gggacggatc tgatcgaacc gcaccggatg gagtattcga
     7141 cggtccaagc gattctcgcc gattttgcgc ggtgggacgt catccccctt ctttccgcat
     7201 tggacggatt ctacgttcac gccgaccacc gcgaccatcc ccgggtggcc gccctgttgg
     7261 ggcgggatta cacacatgtt tataaagatc cttcggaaat tccggatacg atgctgcaaa
     7321 tgaccgcact gtgccgcggc aaccgcgacg agctgttgat caaggcgaaa gagcgctatg
     7381 ctttggacct tcacgtcttt tccaccggcg tccaactgtt tgacatctgt ccgaccaaca
     7441 aaggagagtc gctgaaagag atccgtactc tcctgaaggt tcgggaaaag gacagctggg
     7501 cgttcggcga tgatgaaaac gacttgtcga tgctccgttc agccggaaag ggatacttga
     7561 tgaacaatgc ggcaactcat ctccatgatc agggattnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnggc ttcagcgccg gtaccaaaga ggaagccgtg aaggaagtct
     7741 acttcctcaa cttcaaaccg gaaatcgcat cggtctatga gaccaaggtg gctcccgcct
     7801 ttgaagctga aaccggcatc aagctgaaag tcgttaccgc ggcaagtgga acgtacgccg
     7861 ctacgctgaa aagcgagttg gccaagagca gcccgccgac aatcttccag accaacggtc
     7921 cggtcggatt ggcaggaagc ctcaactatg tttccgactt gaagaacacc cagttctaca
     7981 agattctcag tgacaagtcg atggctctga ccagcgggga tcaagttctt gcaattccct
     8041 atgcggttga aggctatggc ctcatttaca acgatgcgat catgcgcgcc tatttcgccc
     8101 tgccgaacaa aaaggtttcg atcaagagcg ccgaagagat cacctcgttc gctcttctga
     8161 aggccgttgt cgaagacatg acccgaaata aagaccgctt gggaatcaag ggcgtctttg
     8221 cttccaccag catggctccg ggaaatgact ggcgctggca gactcacctg gtgaacgtac
     8281 cgctcgccta tgagttccgc gacaagggaa tccccaccgg aaccaagacc gacaactttg
     8341 agttcaagta cgccaacgag atgaagcagc tttgggacct ctatcttgac aactccacaa
     8401 ccgcccgagg ccttctcggt tcgaagagcg tcgatgacgc gatggcggag ttcgccctcg
     8461 gtcaggcggc gatcgtccag aacggtaact ggggagcggc tcagattctc ggtgtacagg
     8521 gaaacaaagt cgccgacaag gacatcaagt tcatgccgat ttacattggg gtgaagggtg
     8581 aagagcatga tggcctgaac gtcggaacgg agaattacct ctgcatcaac agccaagtga
     8641 gcgccgagaa gcaggccctt gccgaccagt tcctcacctg gctcttctca agcgacaccg
     8701 gtaaggcgat cgtgaagaac gacctgatgt tcatcacgcc gttcaacacc ttcaaggatc
     8761 atgaactgcc gaccgacccg cttgccaagg aagtcatccg ctggatgaac aaagacggcg
     8821 tttccagcat gccttgggcg ttcagcgtca ttccgagcgg ccagtggaag accgatttcg
     8881 gtgccgatct tggccagtac atcaacggca agatgagctg gagcgatgtc gccaagcggg
     8941 ccgtcgcttc ctggaaaacg gaatacgatc tcactaaata acttatcata agtgcattgg
     9001 ggccgtttag atacggcccc tctgtacata aaaggatata tcaatggata agaccttgcg
     9061 gcgatgggcg cccctgttcg tgttacccgc tctcatctct tttatcatcg cgtttctggt
     9121 tccgatgatc atgggattgg tgctttcatt tttcaagttt tcaaccgtca ccgacggcgt
     9181 gtttgtcgga tggagcaact ataagcgaat cttcaccgat atggatttcg tcacatcgct
     9241 ttggatgaac gtccgattca ccgttgtcac cgtgatctcc atcaatatcc tggcgttttt
     9301 cctcgctctg ctgcttactc agggggtaaa agggacgaat gtctatcgga cgatcttctt
     9361 catgccgaac ctcatcggcg gaatcgttct cggctggatc tggctgctca tcatcaacgg
     9421 aatcctcctg ccaaggaatg cgacgatcat caccgatgca aaatacgggt tctgggggct
     9481 cgttgccctg gcgaactggc agaatatcgg atatatgatg gtgatctaca tcgccggaat
     9541 ccagacgatc aaccgtgacc tgatcgaagc ggcccagatc gacggagcaa acagctggat
     9601 tcgcctcaaa agcgttattc ttccctcggt gatgccggcg attacgatct gtacctttct
     9661 caccctgacc agcggcttta aaatcttcga ccagaacctt gccttgacag cgggcaatcc
     9721 cgcccggcag acggagatgc tcgccctcaa catctacaac accttctaca cccggaccaa
     9781 cttcgaaggg gtgggccaag ccaaagcggt cctctttacg atccttgtcg caatcatcgc
     9841 cctgacccag cttgcagcga cgaggcagaa ggaggttgag gcatgagcat caagacccga
     9901 accaactatc ttcacatcgt gatctcgatt cttctggcga tgctggcgat cctgtttgtc
     9961 gctccgatta tcatcgtctt catgaactcg ttcaaatcga agctcttcat ctccagcgaa
    10021 ccgttcgcct ttcccacttc cgaaacgttt accgggtatg cgaactacgt cgagggggca
    10081 agaaagatca acttcttcaa agcattcggg tattcgctct tcatcaccac attcagcgtg
    10141 accgcagtgg ttggcaccac aagcatgctg gcttggtaca tcacccgggt gaaatcgctc
    10201 tttacaacct ttacctacta tctgttggtg ttctcgatga tcgttccctt ccagatggtg
    10261 atgttcacga tgagcaagat agcgaacatc cttcacctgg ataatcccgt cggagtcatc
    10321 gtcctctacg taggcttcgg agcggggctg ggctccttca tgttcagcgg attcatcaag
    10381 acgattcctt taagtctgga ggaagcggct ctcatcgacg gagcaacccc tcctcagacc
    10441 ttttttctgg ttgttcttcc gatgctgaag cccactgcga tcacgatcgc gatcctcaat
    10501 gcgatgtgga tctggaatga ctacctcctt ccgtatctga cgatcggaac ggagtacaag
    10561 acgatcccgg tggcgatcca atacctgagg ggcggctatg gagccgtgga catgggcgcg
    10621 atgatggcga tgctcgttct tgcgatgctg ccgatcatca tcttctatct cttcacccaa
    10681 cgctacatca ttgaaggagt cgttgcagga gcggtgaaag gttaatc
//
LOCUS       DAOSLI010000054        10678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_6077_length_10678_cov_30.313941, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000054 DAOSLI010000000
VERSION     DAOSLI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6077_length_10678_cov_30.313941"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..959
                     /locus_tag="PLF67_07520"
     CDS             432..959
                     /locus_tag="PLF67_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89894.1"
                     /translation="MKKLATLLIILALATGGVFAYDSTLDLSTIVDQTQGMKITASQF
                     TGTTLEALNSATEVTSYTVTAGGEYEVGWISVISNNLTGYYVTASATAMASTAGTTTN
                     YIDYTVSFGTDEFTTTGATTAYLEPSKYALIAQSLGSLTPNSRKITLKISDNALDSAA
                     EGQYTGTVTFFFNAT"
     gene            1259..1873
                     /locus_tag="PLF67_07525"
     CDS             1259..1873
                     /locus_tag="PLF67_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOE89895.1"
                     /translation="MAKSRRDTADSYTLRARKEGYPARSVYKLEEIQKQFAIIKKGDT
                     VLDVGAAPGSWTLYTGNVLLKGTGKIVAVDLNPLNINPIPHTVISYVGDAFGKEIKPK
                     LIEHGPYDAIISDAAPLTTGNRGVDTARSEYLAEQVILLASEHLKAGGNLVVKLFQGG
                     GEQELMKRLRALFTRAKIVKPKACRDDSFEIYLVGVGRKEGEQG"
     gene            1870..2289
                     /locus_tag="PLF67_07530"
     CDS             1870..2289
                     /locus_tag="PLF67_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HOE89896.1"
                     /translation="MKITVIDGQGGSLGKALVAAIKKRFPHEEVFAIGTNALAASAML
                     GSGADAAASGENPVLVACRKSEVIIGPIGLLAADSLHGEITPAMAVAIGQSSAHKILL
                     PITKCNVSIVGKQELSTKEMIEGAVAELASYLVDRSL"
     gene            2337..3428
                     /locus_tag="PLF67_07535"
     CDS             2337..3428
                     /locus_tag="PLF67_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HOE89897.1"
                     /translation="MKVRNDHSFFAFMAFSLEDYVTRYQQPLEEGLSLAVYNGDDPIF
                     THQGHWLHPLFALEEFLLTYEGPRDLLSVHDSAAGKAAAVLQARLGVRRAHIDLISDL
                     AVEYYRAKGIEVSWEKKIERLACQTESLLAEIDDEDEIYRILKRRANLVQGVGVEVAG
                     ISFAYPNSEPLFKNLHFTLPEGGRLIIQGDNGMGKTTLINLLLGRLLPTSGAIRIDGR
                     EPSELPPRTIGYIKQQQTQEQFPVSAREVVAMAVDPSLPRERQTWEIDTALRRTASAH
                     LSSRNFFSLSGGERQRVSLARTLCQKARLLLLDEPTSFLDAKSRTALVGLLHSLTLAE
                     MPTIIIVTHDKELEEDLGWEILRLGGDHE"
     gene            3421..4227
                     /locus_tag="PLF67_07540"
     CDS             3421..4227
                     /locus_tag="PLF67_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOE89898.1"
                     /translation="MNDFLFALTLAPVARGLLAMAIAGLAFPAAGVMVLRLNLIPMRY
                     MLMHGVILAGAISLALNVNTLAISVVLNLILVLLMLRLTKDTRFGFGLSSATAMVFTM
                     AAASLVMHIWDVPAKDTLQLLWGSPFALTWTDISALVALAIIIVLYMFMSFRTISALF
                     FDQEIASSLGMRVRFHHTFMVTLIALVIALAMKLLGALLIDALLILPVLVASKRSESL
                     KRLVLSACIIGFFVSTLGYLLAIYTDMPPSGTIALLSALIYPIPTFKKRS"
     gene            4230..4982
                     /locus_tag="PLF67_07545"
     CDS             4230..4982
                     /locus_tag="PLF67_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89899.1"
                     /translation="MKRIVVLVLAALLSFTLFAQAVPEKQESTVVASTAWTAAFADIA
                     GVDNVDFIAPATMTHPPEYEITVGDVQKINNAEYFLYAGYEVMMKSMGTTIKKDESAL
                     IHIQTNNSIENVRTQSAKLAAIFGTEAENKVRFATYEEGILDGKKQVAAKGLDKLNVY
                     CHAMQVYLAKDLGLNVVGTFGPAPLTAAQLAEIAKGEIDIIIDNIHNPVAPPALEVSP
                     KSRIVTWRNLPARGGRGTLEEMVRSNIAELLK"
     gene            complement(5026..6252)
                     /locus_tag="PLF67_07550"
     CDS             complement(5026..6252)
                     /locus_tag="PLF67_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE89900.1"
                     /translation="MDLKKRAYKLLNDWKGSAYIHGLGVLDQVGEAVATLGRNALVIA
                     NTTRKSATVEKVLASLTDAGVGFGEVVPTAKPNTPKEDVLRLGKVISSRKPEVIVTIG
                     GGSAIDATKAANALATLGGAIEEYFGSDLVTKALKERNLHLLPTVAVMTAASSAAHLT
                     KYANVTDLAAGQKMLIVDNALVPTLSVFDYTSTTTMPKDLTIDGAFDSIAHTLEVYLG
                     SHSETIDLTGTIADCALRLVVENAPILIDDPTNMEAREALGLASDLGGYAIMVGGTSG
                     AHLTSFSLVDIVAHGTACGIMNPYYVVLFGRAIKQQLQVIGPIFGSKDAAPISVAEAM
                     IAFAKSIHAPTKLKDIEGFTEAHITRALAAAKDPQLKMKLQNMPIPMDAADVDTYMAP
                     LLRAAAEGDLTRIKAM"
     gene            6341..7438
                     /locus_tag="PLF67_07555"
     CDS             6341..7438
                     /locus_tag="PLF67_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOE89901.1"
                     /translation="MNMRDIFHIYGTNGFEMTQILLKAIEPEKLIPSQSSTIAIKPNL
                     VVAAKPEGGATTHPEIVIAIIEYLKERGFHNISIVESAWVGDSTKRAFEVNGYYAISS
                     KYDVPLVDVKDDAYVTRTVHGIPIELSKTITETDFLISLPVLKGHCQTSMTCALKNMK
                     GCISERSKRQSHTMGLHKPIAALNALRSADLVIVDSLNGDLDFEEGGNPVRTDRIFAC
                     RDSVLCDTYGASLMGFEAEDIEYIRLAEQFGVGTTNLDFANIIFLNEPSAIAPPPLPT
                     GKATYLNQYIDSRSACSACHGTLVHALKRLDEANQLGSKFNRKICVGQDFKTIMDLAR
                     VGVGTCTKGLGESVPGCPPTALAMLEFLKSL"
     gene            complement(7494..9377)
                     /gene="htpG"
                     /locus_tag="PLF67_07560"
     CDS             complement(7494..9377)
                     /gene="htpG"
                     /locus_tag="PLF67_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOE89902.1"
                     /translation="MAKKKFKTEVTELMQLIIHSLYSHKEIFLRELVSNASDALDKLK
                     YLTLTDERLKNLDYNPRIDITFTEESPRTLTIRDNGLGMDEEELSNNLGTIASSGTKK
                     FLASLTEEQKKDSNLIGQFGVGFYSAFMVAKRIDVRSKKAGTEQAYLWSSTGKGTFDI
                     SESELDGHGTEIVLHLNEDGETYASRWQIEQLIKKYSDHIAYPIFLTYEAVTYDDKKK
                     DKDGKAEKKSETKVEQINSSSALWRRPKSELTDKDYNEFYKQTTYDSDDPLFYVHTKA
                     EGTTEYTTLFFIPKKAPFDMYYADYRPGVKLYVKRVYITDDDKELLPPYLRFVRGIID
                     SEDLPLNVSREILQQNRIMASIRSSSVKKLLGEFQKMSENNPTMFDAFIEQYNRPLKE
                     GLYSDYSNRDALLELVRFKSSTEDGWVSLASYKERMKSDQKSIYYITGGKEEVLKASP
                     LLGAYTKRGYEVLIMSDDIDDIVISQLGFYKELPLKAINKSGAVDDLKEGDEKTKESK
                     ESKALIKKIKKALGNKVKDVVASTRLVDAPAVVVVDENDPTVQMQQLLKMMGQDQGEE
                     ILPILEINLDDPIIRKIETSDDKEYIEDLSSVLLDQALLSEGVMPKDPVAFTRRLQSL
                     LAR"
     gene            9480..10529
                     /locus_tag="PLF67_07565"
     CDS             9480..10529
                     /locus_tag="PLF67_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOE89903.1"
                     /translation="MDLSHPSVARTVNRLRVLNLIAREGAISRAEIARVLDLSKPSTS
                     EIVALLLEENLVMESGKAETASGRKPTNLKLKSDGALVLGIDMDVRTTTYTVANIHGA
                     ILRMEKFPIQEHPDPKTAGAEVIKRILKLTRESKVPFKAIVLTTGGTFSEDRRTLVAH
                     NYWSWEQIPLAEVIEKNTSIPTIFAHTTRSMSFAERWFHKETPSSYLYINWGEHLESA
                     LYINNVDRLSRFEHTPVGQTGLCFCGQIGCLETIAGLSRIGKEDLPHAAMAIGKALIA
                     ADRATAVDAIIIAGPQAREYLPIIQENYQGSTPIIPSILKERSTPLASVAVALDWGIF
                     ERSVLEQAKTATRNG"
BASE COUNT         2390 a         3048 c         2836 g         2404 t
ORIGIN      
        1 actgaaggaa aaggaaaacc ccaaataacg tcgggaaaca aagattttct tggcatagtt
       61 gttgcatatc aaaaagtgta cagagcaaca gtttttttca tactcctcct ttaattttta
      121 gggtgccgaa gccgttttcc atagcattgg caccctactt ttatctccta ggcatgcctg
      181 caaagcggtt ctgtgcgcga gaactcctac catttctatt gctttgtcat atgttttcca
      241 aactagaaat aaaattaaat ctttatttta aaatatatta cgattattat aaaaatatta
      301 tatctgtttg tgttgatttt tatgaaaccc atattgacat aagtttcgta tttcagtacc
      361 atacagatga gcctcaggaa aagggctcat cgaatacttg cacaggagcg tagctcctta
      421 cggagaccta tatgaagaag cttgccaccc tattgatcat ccttgccctt gcgaccggcg
      481 gcgtctttgc ctacgattcc accttggatc tcagcacgat cgtcgaccag actcaaggga
      541 tgaaaataac cgcttcacag ttcaccggaa ccaccctgga ggccttaaac agtgcgaccg
      601 aagtcacttc atataccgta accgccggtg gagagtatga agtcggctgg atttccgtga
      661 tcagcaacaa cctgacaggc tattacgtca cggcaagcgc gacagcgatg gccagtacgg
      721 cgggaaccac caccaactac attgactaca cggtctcttt cggcactgac gagttcacca
      781 ccaccggtgc aacgacagca tatcttgaac catctaaata tgctctcatt gcacagtccc
      841 tgggatccct aacccccaat tcaaggaaaa tcactttgaa aatcagcgat aacgcgctcg
      901 actccgccgc ggaaggtcaa tacaccggaa cggtgacttt cttcttcaac gcaacctgaa
      961 cacgatacat cttgtctgtc ccggctgccc gagcgggcag cctttttact gtgcatttcc
     1021 tgagagctgt tactcttttg cacacctgag gttcttatgc tttgtaccaa gagctgagaa
     1081 agaagagcgc ccggcaaaga actgtcgatt tggcatagta gttgcacggc tgttggtgaa
     1141 cacagttttt tagaacctcc ttaatgtgtg attccttagg tgccagtgag actgcatcac
     1201 cccaactcct cactggcacc atctttatgc gtctgtgggg ttttggtata gtgaacgtat
     1261 ggcgaaatca agacgcgaca ctgccgattc ctataccctt cgggcccgca aagaaggcta
     1321 tcccgcccgc agcgtgtaca agctggagga gatccagaag cagtttgcga tcataaaaaa
     1381 gggcgatacg gtcttggacg tcggggcggc cccgggctcg tggaccctct ataccggcaa
     1441 cgtcctgctc aaagggacgg gaaagatcgt cgcggtggat ttgaatccgt tgaacatcaa
     1501 cccgatcccc catacagtca tctcctatgt cggtgatgcg ttcggaaagg agatcaagcc
     1561 gaaactcatc gaacacgggc cgtatgatgc gatcatcagc gatgccgctc cgctgacgac
     1621 gggaaaccgc ggcgtcgata ccgcccgcag cgaatacctt gccgaacagg tgatcctcct
     1681 cgcatccgaa cacctcaagg cgggcgggaa tctggtggtc aagctctttc aaggcggcgg
     1741 ggagcaggag ctgatgaaga ggctcagagc cctcttcacc cgggccaaaa tcgtgaagcc
     1801 caaagcgtgc cgtgatgact cctttgagat ctatctggtc ggagtcggca ggaaggaagg
     1861 agaacaggga tgaagataac ggtaatcgac ggtcagggcg gcagcctcgg caaagcgttg
     1921 gtggcggcga tcaagaaacg gtttcctcat gaagaggtct ttgcgatcgg aaccaacgcc
     1981 ctcgccgcca gcgcgatgct gggaagcgga gcggatgcag cggcaagcgg agaaaatccg
     2041 gtgttggtgg cgtgtcgaaa aagcgaggtg atcatcggac cgatcggtct tctcgccgcc
     2101 gattcgctgc acggggagat caccccggcg atggccgtag cgatcggaca aagctccgcc
     2161 cataagatcc tgcttcccat caccaagtgc aatgtctcga tcgtcggaaa gcaggagctt
     2221 tccaccaaag agatgatcga aggggcggtc gccgaacttg cctcgtactt ggttgaccgt
     2281 tccctctgag aacgctacac tcaaatcaac cgctccatga aggagcacca aaagccatga
     2341 aggtaaggaa cgatcactct ttctttgcgt tcatggcttt ttcgttggag gattatgtga
     2401 ctcgctatca acaacccctt gaagaaggtc tctcattggc cgtatacaac ggggatgatc
     2461 cgatcttcac ccatcagggc cattggcttc atcccctctt cgcgctcgag gagttcctcc
     2521 taacatacga aggcccccgg gatcttttgt ccgttcacga cagcgccgcc ggaaaagccg
     2581 ctgcggttct tcaagcacgg cttggagtgc gaagagccca catcgatctg ataagcgatt
     2641 tggccgttga gtactaccgg gcgaagggga tcgaagtctc ctgggaaaag aagatcgaac
     2701 ggcttgcatg ccagaccgag agcctgcttg ccgagatcga cgatgaagat gagatctatc
     2761 gaatccttaa acgccgggcg aacctggttc aaggtgtcgg tgttgaagta gccgggataa
     2821 gcttcgccta tccgaacagt gaaccgctct ttaagaatct gcacttcacc ctccccgaag
     2881 gagggcgcct gatcatccaa ggggacaacg ggatgggaaa aaccaccctg atcaacctgc
     2941 tgctcggcag gctgcttccc acctcggggg cgatcaggat cgacggcagg gagccgagcg
     3001 agcttcctcc ccggacgatc ggatacatca agcagcagca gactcaggag cagtttcccg
     3061 tttccgcacg ggaggtcgtg gcgatggccg tcgatccaag cctccccaga gaacgacaga
     3121 cgtgggagat cgatactgcc ctccgaagga ccgcttccgc ccatctgagc tcccggaact
     3181 tctttagctt aagcggcggt gagcgccagc gggtctcgct ggcccgaacc ctctgtcaaa
     3241 aagcccgtct tctcctgctg gatgaaccca cttcgtttct ggatgcgaaa agccgaaccg
     3301 cactggtagg tctgctccac tccctcaccc ttgccgagat gccgacgatc atcattgtca
     3361 cccatgacaa ggagctggaa gaggatctgg ggtgggagat cctacgcttg ggaggtgatc
     3421 atgaatgact ttctcttcgc cctcaccctc gccccggtcg cccgcggcct tttggcgatg
     3481 gcgatcgcag gtctcgcatt tcccgccgcc ggggtcatgg ttcttcggct caacctgatt
     3541 ccgatgcgct atatgctgat gcacggcgtg atcctcgccg gagcgatctc attggcgttg
     3601 aacgtgaaca ccttggcgat cagcgtcgtg ctgaacctca ttctggtgct tctgatgctc
     3661 aggctcacaa aggacacccg cttcggattc ggtctctcca gcgcgacggc aatggtcttc
     3721 acgatggccg ccgcatcgct ggtgatgcac atctgggatg tgccggcaaa agacaccctc
     3781 cagctccttt gggggtctcc tttcgctctc acgtggaccg atatctccgc cctggtcgct
     3841 cttgcaatca tcatcgtcct ctatatgttc atgagttttc ggacgatcag cgctctcttc
     3901 ttcgaccagg agatcgcctc ctcgctgggg atgcgggtcc gtttccacca caccttcatg
     3961 gtgacgctca tcgccctcgt catcgccctg gcgatgaagc tcctcggagc gctcctgatc
     4021 gatgccttgc tgattcttcc cgttctcgtc gcttccaagc gaagcgaatc gttgaagcgg
     4081 ctcgtccttt cggcgtgcat catcggcttc tttgtttcca ccttgggata cctgttggcg
     4141 atctacaccg acatgccccc gagcgggacg atcgccctgc tttccgccct catctatccg
     4201 ataccgacct tcaaaaaaag gagttgaata tgaaaagaat cgtcgttctt gtccttgcag
     4261 cgctgttgag tttcaccctc ttcgcccaag ccgtcccgga aaaacaggaa tccacggtcg
     4321 tcgcctcgac cgcatggacc gcggcgtttg ccgatattgc cggagtcgac aacgtcgact
     4381 tcatcgcccc ggcgacgatg actcatcccc cggagtatga gatcaccgtc ggcgatgtcc
     4441 agaagatcaa caacgccgag tacttcctct acgcggggta cgaagtgatg atgaagtcga
     4501 tgggaacgac gatcaagaag gacgagtcgg ccctcatcca tatccaaacg aacaactcga
     4561 ttgaaaacgt ccggacccaa agcgccaagc tggctgcgat cttcggaacc gaagcggaaa
     4621 acaaggttcg cttcgcgacc tacgaagagg ggattctcga cgggaaaaag caggtagccg
     4681 caaagggtct ggacaagctc aatgtctact gccatgcgat gcaggtgtac cttgcaaagg
     4741 atctcggcct caacgtggtc ggaacgttcg gtccggctcc gctgacggcg gctcagcttg
     4801 ccgagatcgc caagggggag atcgacatca tcatcgacaa catccacaac cccgtcgctc
     4861 ctcccgcttt ggaggtttcc cccaagagca ggatcgtgac gtggcgcaac ctcccggccc
     4921 gcggcggacg gggaactctt gaagagatgg ttcgctcgaa tatcgccgaa ctgcttaaat
     4981 gacgcagaat gttcaaggag gccgaagccg gcctccttgt tccatctaca tcgccttgat
     5041 gcgggtaagg tcgccctcgg ccgctgccct gagaagcggc gccatatagg tgtccacatc
     5101 ggcggcatcc atcggaatcg gcatgttctg cagcttcatc ttcagctggg ggtctttagc
     5161 ggcggcgagc gcccgggtga tgtgggcttc ggtgaaccct tcgatatcct tcagcttcgt
     5221 cggggcatgg atcgacttgg cgaaggcgat catcgcttca gccacggaaa tcggagcggc
     5281 atccttggaa ccgaagatcg gcccgatcac ctgcaactgc tgcttgatcg cccggccgaa
     5341 gagaaccacg taatacggat tcatgattcc gcaagccgtt ccgtgggcga cgatgtcgac
     5401 aagggagaag ctcgtcagat gagctccgct tgttcctccg accatgatcg catacccgcc
     5461 gagatcgctc gccaagccaa gcgcctcccg cgcttccata ttcgtcggat catcgatcag
     5521 gatcggcgca ttctcgacaa cgagacggag agcacagtcg gcgatcgttc cggtgagatc
     5581 gatcgtctcg gaatgagaac cgaggtacac ctcgagggta tgggcgatcg agtcaaaggc
     5641 accgtcgatc gtcaggtctt tcggcatcgt ggtggtggag gtgtaatcga agacactcaa
     5701 cgtcggcacc aacgcgttgt cgacaatcaa catcttttgt cctgcagcaa gatccgtgac
     5761 gttcgcatac ttggtcaggt gggccgcact ggacgctgcg gtcatcaccg ccaccgtcgg
     5821 aagcaagtga agattccgct ccttgagcgc ttttgtaacc agatcgcttc cgaagtactc
     5881 ttcgatcgct ccgccaaggg tggccaacgc gttcgccgct ttagtcgcat cgatcgccga
     5941 cccgccgccg atcgtgacga tcacctcggg cttccgggat gaaatcactt tgccaaggcg
     6001 gagaacatcc tccttcgggg tgttgggctt cgccgtcggc acgacttcac cgaagccgac
     6061 tcccgcatcc gtaagcgaag cgagaacctt ctcgacggtc gcgctcttcc gggtggtatt
     6121 cgcgatcaca agggcgttcc tgcccaaggt tgccaccgct tcaccgacct gatcgaggac
     6181 tccaagcccg tggatatagg cggaaccttt ccaatcattc aacaatttat acgctcgttt
     6241 ttttaaatcc atatcgttcc cctctttcat atactacctg atactgtcct gttcagtcaa
     6301 agattgactg aaccactgtt cccgacttac actcagaaca atgaacatgc gcgatatttt
     6361 tcacatctac ggaaccaatg gtttcgagat gactcaaata cttcttaaag cgattgaacc
     6421 tgaaaaactc attccatccc aatcgagcac cattgccatc aaaccgaacc tggtcgtcgc
     6481 ggcgaagccg gaaggcggag cgacaaccca ccccgagatc gtcatcgcga tcattgaata
     6541 cctcaaagaa cggggattcc acaatatttc aatcgttgaa agcgcctggg tcggagactc
     6601 caccaaacgg gcgttcgaag tcaatggcta ctatgcgatc agcagcaagt acgatgttcc
     6661 ccttgttgac gtaaaagatg atgcctacgt cacccggacg gttcatggaa tccccattga
     6721 gctgagcaag acgattacgg agaccgattt cctcatctcc cttcccgtcc tcaagggaca
     6781 ctgtcaaaca tcaatgacgt gcgccctcaa gaacatgaag ggatgcatct ccgagcggtc
     6841 caagcgtcaa tcccacacca tggggcttca taagccgatc gccgcgctga atgcgctgcg
     6901 cagtgcggat ctggtcatcg tcgacagtct gaacggagat ctggactttg aagagggcgg
     6961 caaccccgtc cgaaccgatc ggatcttcgc ctgccgggac tccgtcctct gtgacaccta
     7021 tggtgcttcg ctgatgggat tcgaagcgga agacatcgag tatatccgct tggccgaaca
     7081 gttcggggtg ggaacgacga acctcgactt tgcaaacatc atctttctca atgaaccgag
     7141 cgcaatcgcc cctccgccgc ttcccaccgg aaaggccacc tacctcaacc aatacatcga
     7201 cagccgaagc gcatgctcgg cctgtcacgg aacattggtc cacgcgttga aacggttgga
     7261 tgaggccaat cagcttggta gcaagtttaa tcgaaagatc tgtgtcggac aggatttcaa
     7321 aaccatcatg gatttagccc gggtcggtgt cggaacatgc acaaagggct tgggtgagag
     7381 cgtgccgggg tgtccgccga ccgcgttggc gatgctggag tttcttaaaa gcctgtaaca
     7441 cacgcgagag cccatgacgg gctctcggtc gtatatatga atgccgttgg tgatcagcgc
     7501 gccagcaggc tttgcagtct gcgggtgaac gcaaccggat ccttcggcat cacgccttcg
     7561 gagagaaggg cctggtccag aagcaccgag gaaagatctt cgatatactc cttatcatcg
     7621 ctcgtttcga tcttcctgat gatcggatca tcaaggttga tctcaaggat cggaagaatc
     7681 tcttcaccct gatcctggcc catcatcttc agaagctgct gcatctgtac ggtcgggtcg
     7741 ttctcgtcga ccacgacgac cgccggagca tcgacgaggc gggtggaggc cacgacgtcc
     7801 ttcaccttgt tcccaagggc tttcttgatc ttcttgatca aagccttgga ctccttgctc
     7861 tccttggtct tctcatcacc ttccttcagg tcgtcgaccg cgccgctctt gttgatcgct
     7921 ttcagcggca gctccttgta gaaaccgagc tgactgatga cgatatcatc gatgtcatcg
     7981 ctcatgatga ggacttcata gccgcgcttg gtgtaggctc cgagcagcgg actggctttg
     8041 agcacctcct cttttccgcc ggtgatgtag tagatcgact tctggtcgct cttcatccgc
     8101 tccttgtagg aagccaggct cacccatccg tcttccgtgg aagacttgaa gcggaccagc
     8161 tccaggaggg cgtctcggtt ggagtaatcg gagtacaatc cctccttgag gggtcggttg
     8221 tactgttcga taaacgcatc gaacatcgtc gggttgttct cgctcatctt ctggaattca
     8281 ccgagcagct tcttcacgct ggaggagcgg atcgaggcca tgatccggtt ctgctggaga
     8341 atttcccgcg agacgttcag gggaagatcc tcgctgtcga tgattccccg gacgaaacgg
     8401 agatatgggg gcagcagctc cttgtcgtca tcggtgatgt acacccgctt cacatagagc
     8461 ttcaccccgg ggcgatagtc ggcgtagtac atatcaaagg gggctttctt ggggatgaag
     8521 aagagagtcg tgtactcggt ggttccctcc gccttggtgt gtacatagaa gaggggatca
     8581 tcgctgtcgt aggtggtctg cttgtagaac tcgttgtaat ccttgtcggt caactcgctc
     8641 ttcggccggc gccagagcgc cgaagagctg ttgatctgtt cgaccttggt ctcgctcttc
     8701 ttctcggctt tgccgtcctt atccttcttt ttgtcatcat aggtcaccgc ttcataggtc
     8761 agaaagatcg gataggcgat gtggtcgctg tacttcttta taagctgttc gatctgccag
     8821 cggctggcgt atgtttcgcc atcttcgttg aggtggagga cgatttccgt cccatggccg
     8881 tccagctcgc tctcggaaat gtcgaacgtt ccttttccgg ttgaggacca gaggtatgcc
     8941 tgctcggttc ccgctttctt ggagcggaca tcgatccgct tggcgaccat gaaggcgctg
     9001 tagaatccga caccgaattg accgatcagg ttggagtctt tcttctgttc ttccgtcagg
     9061 gaagcgagga acttcttcgt tccgctggat gcgatcgttc ccaggttgtt ggaaagctct
     9121 tcctcatcca ttccgagacc gttgtcacgg atcgtgaggg tccggggaga ttcttcggta
     9181 aaggtgatat cgatccgagg gttgtaatca aggttcttca gccgctcgtc ggtgagggtc
     9241 agatatttca gtttgtcgag ggcatcggat gcgttgctga ccagttcccg caggaaaatc
     9301 tctttgtgcg aatacaggga gtgaataatg agctgcatca gctcggtcac ttccgtcttg
     9361 aactttttct ttgccattat atgttcatac ctccaaggga atatatcaaa agatactgag
     9421 atttgttcca atcggcaata aaccttaaca aaacggtcgt cggtggggta tgctggggca
     9481 tggacctatc ccacccttcc gtggccagaa ccgtcaaccg cctccgagta ctcaacctta
     9541 tcgcccggga aggagcgatc agccgggcgg agatcgcccg agtgttggat ctgagcaagc
     9601 cctcgacaag cgagatcgtc gcgcttcttc tcgaagagaa cctcgtcatg gagagcggca
     9661 aagccgagac cgcttccggc cggaagccga ccaatctgaa gctcaagagt gacggagcgc
     9721 tggttctcgg catcgacatg gatgtcagga cgaccacata cacggtcgcc aacatccacg
     9781 gtgcgatcct cagaatggaa aagttcccga ttcaggaaca ccccgatccg aagacggccg
     9841 gagcggaggt catcaagcgg atcctcaagc tcacccgcga aagcaaggtt cccttcaagg
     9901 cgatcgtcct caccaccggc ggaacgttca gcgaggatcg aaggactctc gttgcccaca
     9961 actactggtc atgggagcag atcccccttg ccgaggtgat cgaaaagaac acctcgattc
    10021 cgacgatctt cgcccatacg acccgttcga tgagcttcgc cgaacgctgg ttccacaagg
    10081 aaaccccctc ttcctatctc tacatcaatt ggggcgaaca cttggagagc gctctgtaca
    10141 tcaacaacgt cgaccgcctc tcccgcttcg aacacacccc ggtcggtcag acggggctct
    10201 gcttctgcgg acagatcggg tgtctggaaa cgatcgccgg tctctcgagg atcggaaagg
    10261 aagatctgcc ccatgccgcc atggcgatcg gaaaggccct catcgcggcg gatcgggcga
    10321 ctgcggtgga tgcgatcatc atcgccggcc cccaggcaag agaatacctt ccgatcattc
    10381 aagagaatta tcagggctcg accccgatca tcccaagcat cctcaaagaa cggagcacgc
    10441 cgcttgcttc cgtcgccgtg gcgctggact ggggaatctt tgaacggtcg gtgcttgaac
    10501 aagcaaaaac cgctacccga aacggatagc ggtaacctcc ttgagagaac ctcaatactc
    10561 ctccaagctc gaggtatcag acctgccgct ggaaaaatcg accaggtccg agcaacgaca
    10621 tccttattat tgcatggatt ctgtaagctg taaaacaaat attgccgatt atccgtat
//
LOCUS       DAOSLI010000055        10152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_6435_length_10152_cov_23.227493, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000055 DAOSLI010000000
VERSION     DAOSLI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_10152_cov_23.227493"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..462)
                     /locus_tag="PLF67_07570"
     CDS             complement(151..462)
                     /locus_tag="PLF67_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89904.1"
                     /translation="MEPSGADRKRIEQLYEMVSAVTDRSPKDTIRLHVHQHVATLNAS
                     LFEVLSSDNVLVEQRDSIIQGLEELLERSRRKRLELNGLAKTKKASDAVLDYNLEQLF
                     S"
     gene            747..980
                     /locus_tag="PLF67_07575"
     CDS             747..980
                     /locus_tag="PLF67_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89905.1"
                     /translation="MKQSHENKEREASILVNIKAAVFDGEIGGTTSMSFKRFVLPFWE
                     DRSDLHFWPHRRSLSGFNLGLERDLLVAYFCSE"
     gene            complement(1050..1811)
                     /locus_tag="PLF67_07580"
     CDS             complement(1050..1811)
                     /locus_tag="PLF67_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOE89906.1"
                     /translation="MNTFNAAIMTSRQLEAVLNKSNRLIIPLASFEALGTHGPLGADF
                     LVAGHVTPLIAEKAQAVYLPVIPYGDTLELPQIAGNVMIPTDVLSGYYMGVARSFLRQ
                     FPSLSIYFLTFHSLNNRAADNVCRKLAFEGHKPFLIDWWRSVGMGTGDVLDDQEWGTG
                     HGGEMITSVLLHVAPEAVSMEEETNREPKKQFGYYRDHLSWTSSPFAAYTTFADYCEG
                     SSWGVLDNATAEKGEILVKRAVEAISSFIKNSPAQ"
     gene            complement(1808..2824)
                     /locus_tag="PLF67_07585"
     CDS             complement(1808..2824)
                     /locus_tag="PLF67_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE89907.1"
                     /translation="MSKKRFGVGIRGTGQVAVQHVLAVQANPHSYVAAVCGRSKEKAE
                     EFIALHTPGAKAYGEYEAMLDDQSVDIVSECMPNHLHSKEGVLALDAGKHLMLEKPAG
                     NTPEETKALLDAALRSDKKTVVSFVLRWHPMVQNIKSLLNRNAFGEVYYAQADYWHGI
                     SPRFSSYQWIRQRQYAGGAMITGGSHATDIIRYLHGEVAEVHAFATKARDDFDYLTTY
                     VAAVRFVDGSVGKLSASLDGVASPYQFNIDLLGTKGLCRDNRFYARDLFPYQDDWMEF
                     ASITPNSGAVDHHPFKEEFNDLIDAIINDGKALCTVEDACKSMDIVHAINESVSTKKP
                     VLLS"
     gene            complement(2827..3588)
                     /locus_tag="PLF67_07590"
     CDS             complement(2827..3588)
                     /locus_tag="PLF67_07590"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89908.1"
                     /translation="MSEKTLLSIKNLKKVFPLQGEELVALGSVSFDVYPGEFVTIVGP
                     SGCGKTTLLKIIAGLLPKSEGEIHVDEAMFDPTREVGFVFQKALLLNWRRVLDNVLLP
                     IEILKLDKKKYRSKAMEMLELVGLKGFENAYPNELSGGMQQRVSLARALIHDPKLILM
                     DEPFGALDAITREKMNLELQRIWSESKKTIIFITHEINEAVFLSDRVVVMSARPSRMV
                     GAVDIDFPRPRKLEVRTTPQFGEYAVKLYKMLEIT"
     gene            complement(3659..4681)
                     /locus_tag="PLF67_07595"
     CDS             complement(3659..4681)
                     /locus_tag="PLF67_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89909.1"
                     /translation="MRKKVLMIGMVLVLAASMMWAAGVPESGTEPKKLEKVDFLLNWT
                     IAGDHSPYYVAQKFGWFAEEGLDVNIIVGQGSGYSVQAVDSGKADIAISDAPVPITSR
                     EKGANVKIIGIIFDKHPNCSYFWKDSGIKVPQDLTGKTVAVPATDGHKVMWPAFAKQI
                     GVDPNSVKFVNIDPAAKVSALASRKADVVFELFTGKPFMEKAIPADQLGYFVWADYGF
                     NAYAHSYIASDKTIKERPELLKKFLKVAYRAWEYTLNNPEEAIEILSEYHPINKVDYL
                     GNLKVCMEFFKTDRYKNYGIGYIDPQRIRETYSLVDQYQQKLSFPVEDCFDASFLPSP
                     MYKYNF"
     gene            complement(4705..5529)
                     /locus_tag="PLF67_07600"
     CDS             complement(4705..5529)
                     /locus_tag="PLF67_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89910.1"
                     /translation="MKKNGQKAKSAWCKKAVEAIQKNYRSVLVILGFFLLWEFSVVWF
                     KVPEFVLPPPSAALKHLFIKQADANYNWPLHIRTTLQEFILGFCVTAVLGIGLAIIFV
                     WSKKVKDMVLPLFTFINSLPIIAIAPLILLWMGYGIKTNILIAFLVSFFPVVINTMTG
                     LDSIEDDLLDLIRYLNASKLQLFIKIRIPNSLPYIFSGLKICSTMSIVGAIVGEFIAS
                     DRGLGYIIISAQYTMSTPPIFSSLIVISLAGAGLYWVVALAERLIMPWAFKKEQKG"
     gene            complement(5526..6311)
                     /locus_tag="PLF67_07605"
     CDS             complement(5526..6311)
                     /locus_tag="PLF67_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89911.1"
                     /translation="MGKGIKTFIKNAAPKAFNFLLFLVIWQLIVVVFKIKVFILPSPI
                     RVLQHLFYPPIAAKYNWWLHVGATFMEVIVSFVATAIAGIFIAVMICWSPVMNQLLSP
                     IIILFNSLPKIALAPLFLVWFGYGFLPNILVAFMVAFFPVVINSAAGLMAVEEDLLDL
                     VNYLNATKAQIFIKIRIPNSLPYIFAGLKISATMCVVGSIVGEFIAADKGLGYLLRDA
                     QAFIDTPTMFACLFLLSIIGLALFFAIGQLERLVMPWNRRREA"
     gene            complement(6296..7708)
                     /locus_tag="PLF67_07610"
     CDS             complement(6296..7708)
                     /locus_tag="PLF67_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOE89912.1"
                     /translation="MSDLDIRIDDCELYFCPIETRVPLKFGPETTTHVIEARTAIHVR
                     SKGGAEAVGRGETPLSVAWVWPSSLAYSYRQNRLQDFCRVLCELWRTNADSGHAMEIG
                     HRFIEGPLKDVWNKENARCNADEKMPWLAALVCNSLFDLALHDAYGNVHKINTWKTFT
                     KDYMNHDLSRYFGEEHGKPFVGKYPADFFAEGADAATSLVAWHLVGAKDILEPEGYDG
                     PVVEDGYPFFLTDWIARDGLTCLKVKLTGNDRDWDYTRLIDVGRIANESGVLWLTADF
                     NCTVEDPAYVCDILDQLLLEQPEIYAKLLYVEQPFPYDIIAHPIDVRAVSARKPLFMD
                     ESAHDWHHVVLGRELGWSGVALKTCKTLSGALLSLCWAKVHGMTLMVQDLTNPMLAMI
                     PHVSLAAHAGTIMGVEANAMQFYPMASIHEERIHPGLYVRREGKVDFSTLGRHGFGYR
                     YDEIEDARIRAKGEDPWVKA"
     gene            complement(7985..8875)
                     /locus_tag="PLF67_07615"
     CDS             complement(7985..8875)
                     /locus_tag="PLF67_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOE89913.1"
                     /translation="MYLLTGITDEAADRIEDQVAVLHTLGWSAMELRTVDSKLAHELT
                     DEEFSRVADYLEEQQISVPCFASGIANWAKPVHEDFNVTKSAVKRSITRMNRLGSKLV
                     RIMSYQVLQDADGRILEDQMEEERFRRLNWVTQAFLDQGMTPVHENCHTYGGLSSSHT
                     LRMLEHCPGMKLVFDTGNPPITIDADSPFPYTAQNSFEFYNAVKEHVVHVHVKDAYWS
                     SDLEKVEHYTFPGEGRGDVRKILAALQQDGYKGYYSIEPHMEVVFHDSKAVSTKDRRI
                     ANFVEYGRRLERLLAETKPH"
     gene            complement(8968..>10152)
                     /locus_tag="PLF67_07620"
     CDS             complement(8968..>10152)
                     /locus_tag="PLF67_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOE89914.1"
                     /translation="LTLIEQEQSKATRAFIAKQLVLSRTTASSIISQLMQLELVKELD
                     SSINGRGRPAIPLRLTESIWYALGASYHSETWNFLLSDLYGKPVATHQVPTGPINPSH
                     FIATLLEGIDSMLDRVPGRLLPLIGIGTPGLVNSTTGVISRADDLGWKKVAVGEPVTK
                     HTGLPCIVMNRHRAAGLAEAQYGSGRGVKNLIYIGVETGISAAFINDGILLEGADYSA
                     GEIGHMLIDPNGPLCGCGRYGCLQAFSSSQALVRLVEERYRSLKSLGERVEPNILWDV
                     LSEGRLTGVFIAHEAHKGNEIALSCMKKIAVQLGIASSNLINILNPQKIIIGGGLSNA
                     GPLLTELIRSEASSRAMESPFSTVNIEQSILGSWSGALGAASLALKRKIPLAQRWFDS
                     RK"
BASE COUNT         2542 a         2731 c         2327 g         2552 t
ORIGIN      
        1 gtccaactca attgtgtcag cagtgttgac actttttctc gattcttgta aagccttgta
       61 ttttatgact ttatttatat aacaagaaga cagatttgtg ataatgcatt gtatcgctgc
      121 tacagttctg atgatgcgag accttctctt ctaagaaaaa agctgttcaa gattatagtc
      181 caatacggca tccgatgctt tctttgtttt ggccagccca ttcaattcaa gcctttttct
      241 ccgagatctt tccaaaagtt cttcaagacc ctgaataatg gaatcacgtt gctccacaag
      301 aacattatcc gatgaaagca cttcaaacaa agaagcattc agcgttgcga cgtgctgatg
      361 aacatgcaac cgaatcgtat ccttgggaga cctgtcggtt actgcactca ccatttcgta
      421 caattgctca atccgcttcc tgtccgcacc tgaaggctcc aacgtcccat ccagccattc
      481 gtagatcgtg atccgactgg ctccgaatat ccgagcccat tcagccttgc tgaactgaaa
      541 gaaccgatga agcgtgttga actgttcgta gatatatgtg atggcagcct cccctatagg
      601 atgaatatag taacagccgg agcctgcagc aagtcaaaaa ccgaagatta tgaccgttta
      661 agcaaccttt tacaaagtga ccgagcaaag gttgttgcac ttaccctctc cttgggccct
      721 tcttgcccat atgacaggat gccgtgatga aacagagcca tgaaaacaag gagagggaag
      781 cctcgattct ggtgaatatc aaagccgccg tgtttgacgg ggagattggt gggacaacct
      841 caatgtcctt caagcgcttc gtgcttcctt tttgggaaga tcgatccgat ctgcatttct
      901 ggcctcacag gagaagcctg tcgggtttca accttggttt ggagagagat ttattagtcg
      961 cttacttttg ttctgaataa tgagtagtaa tatgtacccg ggcagattac ccgggtacat
     1021 gacaagcaac acttttatcc ttcaaagacc tattgagccg gagagttctt gatgaaggag
     1081 ctgatggctt ccacggctcg tttgaccagg atttctccct tttccgcagt ggcattatcc
     1141 aatacgcccc aggaagagcc ttcacagtaa tcggcaaagg tcgtgtatgc ggcgaacggg
     1201 cttgaagtcc aggatagatg atcccggtag tatccgaatt gctttttcgg ctcccggttc
     1261 gtttcctctt ccatgctcac cgcttcgggc gccacatgga gcagcacgct ggtgatcatc
     1321 tctcccccgt gtcctgtacc ccattcttgg tcatcaagca catcgcccgt tcccatcccg
     1381 accgaacgcc accagtcgat caggaacggc ttatgccctt cgaaagcgag ttttcggcaa
     1441 acattgtcag ccgcccggtt gttcagtgag tggaaggtca ggaaatagat cgacaacgaa
     1501 gggaattggc gcagaaacga tcgggcgacg cccatgtagt agccgctgag gacatcggtc
     1561 ggaatcatca cgtttcccgc aatctgcggc agctcgagcg tatccccata cggaatgacg
     1621 ggaagataca ccgcctgagc tttctcggcg atcaggggag tgacgtgtcc ggccaccaga
     1681 aaatccgcac ctaaaggtcc atgagtcccc aacgcctcga atgaggcaag ggggatgatg
     1741 agccgattcg acttgttcag aaccgcttcc aactgccggg aagtcatgat tgccgcattg
     1801 aacgtgttca tgacagcagc accggttttt tggtcgacac cgattcgttg atcgcatgaa
     1861 cgatatccat cgacttgcat gcgtcttcca cggtgcacag ggcctttccg tcattgatga
     1921 tcgcatcgat caggtcattg aactcttcct tgaacggatg gtggtcaacg gcgccggagt
     1981 tgggcgtgat cgaagcgaac tccatccagt cgtcctgata aggaaacaga tcccttgcat
     2041 aaaaacgatt atcgcggcac agccctttgg ttcccagcag atcgatgttg aactggtacg
     2101 gggaagccac cccgtcgagg gaagcggaca gttttccaac ggatccgtcg acgaatcgca
     2161 cggcggccac ataggtggtc aaataatcaa aatcatctct ggccttggtg gcgaatgcat
     2221 ggacttcggc aacctctcca tggagatagc gaatgatgtc cgtggcatga ctgcccccgg
     2281 taatcatcgc cccgccggca tactgtcgct gccggatcca ttggtagctg ctgaagcggg
     2341 gggaaatccc atgccagtaa tcggcctgag cgtaatagac ctctccaaac gcgttcctgt
     2401 ttaaaagact ctttatgttc tgaaccatcg gatgccatcg aagaacgaag cttaccaccg
     2461 tcttcttatc cgagcgcaac gccgcatcta aaagcgcctt ggtctcttcc ggtgtatttc
     2521 ccgccggttt ctccagcatc aggtgcttcc cggcatcgag ggcaagcaca ccctccttgg
     2581 agtgcagatg gttcggcatg cactcggaaa cgatatcgac cgattgatca tcaagcatcg
     2641 cttcgtattc accatatgct ttcgcaccgg gggtgtgcag ggctatgaac tcctcggcct
     2701 tttcttttga acgcccgcag acggcagcca catatgaatg cggattcgct tgaacggcca
     2761 ggacatgctg tacggctacc tggccggttc ctctgatacc aactccaaat ctcttcttac
     2821 tcactactat gttatctcca acatcttgta tagcttgacc gcatactcac caaactgcgg
     2881 ggtggtacgc acctccaatt tccttggcct cgggaagtcg atgtccacgg ctcccaccat
     2941 tcgggacggc ctggccgaca tcaccactac acgatccgac aggaataccg cctcgttgat
     3001 ttcatgagtg ataaagataa tcgttttctt cgactccgac caaatccgtt gaagctccaa
     3061 gttcatcttc tctctggtta tcgcatccaa agcaccgaaa ggctcatcca tcagaatgag
     3121 cttcggatca tggatcaatg cccgggcaag ggagacccgt tgctgcattc caccggaaag
     3181 ctcattcgga tatgcattct caaatccctt caatcctacc agctcaagca tctccatcgc
     3241 cttgcttcgg tatttcttct tgtcgagctt caggatttca atcggaagca acacattgtc
     3301 caatacccgc ctccagttga ggagaagtgc tttttggaaa acaaaaccaa cttcgcgcgt
     3361 tggatcgaac atcgcttcat caacatggat ctcgccctcg ctcttgggca ataatcccgc
     3421 aatgattttc agcaatgtgg tctttccgca acccgatgga ccgacgatgg tcacaaactc
     3481 tccgggatac acatcgaagc tgacgctccc cagtgcaaca agctcctcac cctgaagcgg
     3541 aaacactttc ttcagattct taatcgacag caacgtcttt tcactcaaag caaaccttcc
     3601 tatcgatttc ccagcgcgag gctgggaaat cattcatgtt ttcctaacag gcgcgttctt
     3661 agaagttgta tttgtacatc gggctcggca agaagctcgc atcaaaacaa tcttccacag
     3721 ggaacgaaag cttctgctga tattggtcaa ccaagctgta cgtctctctg atgcgctgcg
     3781 ggtcgatata cccgataccg tagttcttgt accgatccgt cttgaagaac tccatgcaga
     3841 ccttcaagtt tccgagatag tccactttat tgatcgggtg gtattcggag agaatctcga
     3901 tcgcttcctc aggattgttc aaggtgtatt cccatgccct gtaggcaacc ttcaagaact
     3961 tcttcaaaag ctccggccgc tccttgatgg tcttgtcact ggcgatatag gagtgcgcgt
     4021 acgcattgaa accgtagtcc gcccagacga aatatcccaa ttgatcggca ggaatcgcct
     4081 tctccatgaa cggctttccg gtgaaaagct caaagaccac atcggccttt ctgctggcaa
     4141 gcgccgaaac ttttgccgcc gggtcgatgt tgacgaattt cacgctgttg ggatccacgc
     4201 cgatctgttt ggcgaatgca ggccacatga ccttgtgccc gtcagtcgca ggaacggcta
     4261 ccgtcttgcc ggtcaagtcc tgtggaacct tgattccgct atccttccag aagtagctgc
     4321 agttcgggtg tttgtcgaag atgattccga tgatttttac gtttgccccc ttctcgcggc
     4381 tggtgattgg caccggagca tctgaaatcg cgatatccgc ttttcccgaa tcaacagcct
     4441 gtactgaata tccggatccc tggcccacga tgatattcac gtccaagcct tcctcggcga
     4501 accatccgaa cttctgtgcg acataatacg gagaatggtc gccggcaatc gtccagttca
     4561 gcagaaaatc caccttttcc agttttttcg gctccgtacc actctcaggt acccccgctg
     4621 cccacatcat tgatgcagca agtacgagaa ccataccgat catcaatact ttctttctca
     4681 taccaagcct cctttagctt tcctctaacc tttttgttcc tttttgaatg cccatggcat
     4741 tataagacgc tctgccaacg ctaccaccca atacagtccc gccccagcca atgaaataac
     4801 gatcaatgaa gagaagatgg gaggagtgct catcgtgtat tgcgcactga tgatgatgta
     4861 acccaaccct cggtcggaag cgatgaattc tccgacgatc gcccccacga tgctcatcgt
     4921 ggaacatatc ttcagtccgg aaaaaatata cggcaacgag ttgggaattc tgatctttat
     4981 gaacaactgc aatttgcttg cattcaaata tcggatcaaa tcaagcaaat catcctcgat
     5041 tgaatccaac cccgtcatcg tgttgatcac gacggggaag aagctcacga gaaacgctat
     5101 gaggatgttc gtcttgatcc catatcccat ccacagcaaa atcaacggtg cgattgcaat
     5161 aatgggaaga ctgtttatga atgtgaataa cggcaacacc atatctttaa cttttttcga
     5221 ccagacaaag atgattgcca gcccgattcc aagcaccgct gtcacgcaaa atcccagaat
     5281 gaactcttgg agagtcgtcc tgatatgaag cggccagttg tagttcgcat cggcttgttt
     5341 tatgaacagg tgcttcaatg ccgccgaggg aggaggcaga acaaactcag gaaccttgaa
     5401 ccagacgaca ctgaattccc aaagaaggaa aaagccgagg attaccagga ctgaacgata
     5461 gttcttttga attgcttcga cagccttttt gcaccatgcg cttttcgctt tctgcccatt
     5521 ctttttcatg cttcacgtct ccggttccat ggcatcacca gtcgttccaa ctgtccgatt
     5581 gcaaagaaaa gcgccaaccc gatgatggag aggagaaaca ggcaggcgaa catcgtagga
     5641 gtatcgataa atgcctgggc atcccgcagg agatacccca atcctttatc cgctgcgatg
     5701 aactcgccta cgatcgatcc gacgacacac atggttgcac tgattttcaa cccggcaaaa
     5761 atgtagggaa gcgagtttgg aatgcgaatc ttgataaaga tctgggcttt tgtcgcattc
     5821 aagtagttta ccaaatccaa caagtcttcc tcgaccgcca tcaagcccgc agcggagttg
     5881 atgaccacgg gaaagaacgc aaccatgaac gccacgagaa tgttgggaag gaatccatac
     5941 ccgaaccaaa ccagaaacaa tggcgccaat gctatcttcg gcaatgagtt gaagagaatg
     6001 atgatcggag aaagcaactg attcatcacc ggggaccagc agatcatcac cgcgataaaa
     6061 attccggcta tcgctgttgc aacaaagctc acgatcactt ccatgaatgt cgccccgaca
     6121 tggagccacc aattatactt tgcggcaatg ggaggataaa aaaggtgttg cagaactcgg
     6181 atgggagaag gcagaatgaa cacctttatc ttgaacacga cgacgatgag ttgccagatg
     6241 acgaggaaga gaaggaaatt gaacgctttc ggagcagcat ttttgataaa tgtctttatg
     6301 cctttaccca tggatcctca ccctttgccc gtatacgcgc gtcctcaatc tcatcatatc
     6361 ggtagccgaa tccatgcctg cccaatgtcg agaagtcaac ctttccctct ctgcggacat
     6421 acagacccgg gtggattctc tcttcatgaa tcgatgccat cggataaaac tgcattgcat
     6481 tcgcctcaac gcccatgatc gtcccggcat gtgcggcaag actgacatga ggtatcatcg
     6541 ccagcatcgg attggtcaaa tcctgaacca tcaacgtcat tccatgaacc tttgcccaac
     6601 agaggctcag caaagcgccg gaaagcgttt tgcacgtctt caacgccact ccgctccagc
     6661 ccagctcacg accgagcacg acatgatgcc agtcatgggc gctctcatcc atgaacagcg
     6721 gttttcgggc gctcacggca cgaacatcaa tgggatgagc tatgatatca tatggaaacg
     6781 gttgttccac atagagaagc ttcgcataga tttccggttg ttcaagaagc aattggtcta
     6841 aaatgtcaca cacgtaggcc ggatcttcaa cggtgcaatt gaagtccgca gtgagccata
     6901 aaaccccgct ttcattggca atccgcccaa catcaatcag tcgggtataa tcccagtccc
     6961 gatcgtttcc cgtcagcttc accttgagac aggtcagtcc gtcccgggcg atccagtcgg
     7021 tcaggaagaa gggataccca tcctcgacaa caggcccgtc atacccttcg ggttcaagga
     7081 tgtcctttgc accgacaagg tgccaggcga ccagcgatgt ggcagcatcg gctccttccg
     7141 caaagaagtc ggcggggtat ttgccgacga acggcttgcc atgttcctct ccaaagtacc
     7201 gggagagatc atgattcata tagtccttcg taaaggtttt ccacgtattt attttatgga
     7261 cattaccata tgcatcatgc agagccaaat caaaaagcga gttgcagacc aaggccgcca
     7321 accaaggcat cttctcatct gcattgcagc gcgcattttc tttgttccaa acatccttca
     7381 gcggaccttc aatgaaccga tgccctattt ccattgcgtg tccgctatcc gcattcgttc
     7441 tccacagttc gcaaagaacc ctgcaaaaat cctgcagccg attctgacgg taggaatagg
     7501 caagcgagct tggccagacc cacgctacac tcaacggggt ttcaccccgt ccgactgctt
     7561 cagcaccacc ttttgagcgg acgtgaatcg ctgtgcgcgc ttcaattaca tgggttgtgg
     7621 tctcaggacc gaacttcaga ggaactcgtg tctctatcgg acagaaatag agctcacaat
     7681 cgtcaatacg gatatccaaa tcactcattg gcgctcctta cagtaaaaag tctaccacag
     7741 ttccctatta tgtcaatctt ctgaacaaaa ttgatatcta tctttctaag gatgaattaa
     7801 gatggttttt ccaaatgacc aaggttctga aaatcattgt ttttcatgca tgagccggcc
     7861 atggttccga gactgacggc atcatcgcat gccgatactc cgcaccgctc cctcatgact
     7921 gtgacagcaa cagacagtca attgtaaaaa ggttgagcag gggattgatt ttcaagaatt
     7981 caagctaatg aggtttggtt tcggcaagca gccgttcgag tcgtcttcca tactcgacaa
     8041 aattggctat tcggcgatct ttcgtgctga cggctttact gtcatgaaac acgacctcca
     8101 tatgcggttc aatgctgtag taccctttgt atccgtcttg ctgtaaggcg gcaaggattt
     8161 ttcttacatc ccctcgccct tcacccggaa acgtgtaatg ttccaccttt tccaaatccg
     8221 agctccaata agcatccttg acgtggacat gaacgacatg ctccttgaca gcattgtaga
     8281 actcaaacga gttttgggca gtatagggaa acggagaatc ggcatcaatg gtgatggggg
     8341 gatttcccgt gtcaaacacg agcttcattc ccggacagtg ttcgagcatg cgcaaggtat
     8401 gactgctgct taacccacca taggtgtggc aattctcatg aaccggagtc atcccctgat
     8461 caaggaaggc ttgtgtaacc caattcaggc gtctgaaacg ctcttcctcc atttgatcct
     8521 ccaggattcg cccatcagcg tcttggagaa cctgatagct catgattcga accagcttgc
     8581 ttcccaatcg gttcattcgg gtgattgagc gctttacagc ggatttcgtt acattgaaat
     8641 cctcgtgtac cggctttgcc caattcgcaa ttccgcttgc gaagcagggt acgctgatct
     8701 gttgttcttc aaggtaatct gcgactcggg aaaactcttc atctgtgagc tcatgagcga
     8761 gcttggaatc aacagttctc agctccatcg cactccaacc aagcgtatga agaactgcca
     8821 cctgatcttc gattcgatca gcggcttcat cggtaattcc agtcaataga tacatatttt
     8881 gctcctatcc gatcatcagt tattatacac ttctttgact ctaccatcat ttattaattt
     8941 gtcaatcttc tgtacaaaag cttcagttca cttccggctg tcaaaccagc gttgggctaa
     9001 aggtattttt ctcttcaaag cgagactcgc cgcaccaagc gcaccggacc agctgccgag
     9061 gatgctttgt tcgatattga ccgttgagaa gggagattcc atcgctcgtg atgatgcttc
     9121 acttctgata agctccgtaa gcaacggccc cgcattggaa agaccgcccc cgataatgat
     9181 tttttgcgga ttcaatatgt tgatcagatt tgaactggcg atgccgagct gaacagcaat
     9241 ttttttcatg cacgaaagag ctatttcatt ccctttatgc gcttcatgag caatgaagac
     9301 tccggtaagc cttccctcac tcaatacatc ccataatata ttcggttcga ccctctcacc
     9361 caatgatttc aaggagcgat atcgctcttc aacgagacgc accagggctt gactggagga
     9421 aaatgcctgc aagcatccgt atctgccgca tccgcaaagg ggaccgttcg ggtcaatcag
     9481 catatgaccg atttctccgg cactatagtc tgcaccttcg agaagaatcc catcattgat
     9541 gaacgctgca ctgatgccgg tctccactcc gatgtagatg agatttttta cccctcgccc
     9601 cgagccgtat tgggcttcgg ccaaacctgc agctctatgt ctgttcatta ctatgcatgg
     9661 cagcccggtg tgtttagtga ccggttctcc cactgcaacc tttttccaac ccagatcatc
     9721 ggcacgagag atgactccgg tggtgctgtt gaccaatccg ggggttccga tgccaatcag
     9781 aggcagaaga cgtcccggaa cacgatcgag catagagtcg attccttcca agagggtggc
     9841 aatgaaatgg gaagggttaa ttggaccggt aggaacctgg tgagttgcta ccggtttacc
     9901 atacaagtca ctgagcaaaa agttccatgt ttcactatgg taagaagcac cgagagcata
     9961 ccagatcgat tccgttaatc tgaggggaat ggcgggcctt cctcgcccat tgattgagga
    10021 atcaagctcc tttacaagct ccagttgcat taattgtgag ataatgctgg aagccgtggt
    10081 tctgctcagc acaagttgct tggctatgaa agctctcgtt gcctttgact gttcctgttc
    10141 aatcaacgta ag
//
LOCUS       DAOSLI010000056        10110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_6465_length_10110_cov_30.870711, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000056 DAOSLI010000000
VERSION     DAOSLI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6465_length_10110_cov_30.870711"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLF67_07625"
     CDS             complement(<1..240)
                     /locus_tag="PLF67_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HOE89915.1"
                     /translation="MSKISIRLLDDREMRAVWDERNAGWWFSVLDIIGVLRNEDDPEK
                     NRNYWKYLKAKLKREGNQGAVAKVEKATASFYVRQS"
     gene            complement(274..753)
                     /locus_tag="PLF67_07630"
     CDS             complement(274..753)
                     /locus_tag="PLF67_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89916.1"
                     /translation="MTKRQRTLLIRVGAIILILLFAVFLFHIGRQHTVLIDNNTVTRD
                     GVELQALKLVEVQVGKKGEVLEMTPRIRDKAVVVGQRHTVKVVYTDASWNEHVHELSF
                     RIPLMQNMVLISLPALMKNPEDVSFWLENFEGLAVAQSDAPQEEDVVFDELAGFDSF"
     gene            complement(750..1862)
                     /locus_tag="PLF67_07635"
     CDS             complement(750..1862)
                     /locus_tag="PLF67_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89917.1"
                     /translation="MRELSTKQKILRFMANNTVPLLFVVIIAFGVPLSGYSPTYLAGE
                     VISRISRNTFLIVSLLIPILAGMGLNFGMTLGAMAGQIGLIFTTDWGIVGIPGILLAM
                     IVSTPLSILLGWWCGKILNMAKGREMVTSYIIGFFMNGIYQLVVLYGMGPLIKIISPN
                     LLLPRGYGIRNTVNLIGIRKSLDNFLMIRIGGVPIPVGTFLVIALLSVFILWFKKTKL
                     GQDMRAVGQDMEVARAAGIRVEHTRIMSIIISTVLAGYGMIIYLHNIGTLNTYNSHMQ
                     IGMFSVAALLVGGATVASAKIRNVFLGVVLFHLMFIVSPMAGKRLIGQAQLGEYFRVF
                     VSYGVITTSLVLYEVRKKRERGMSGLALAQDQEVTS"
     gene            complement(1859..2920)
                     /locus_tag="PLF67_07640"
     CDS             complement(1859..2920)
                     /locus_tag="PLF67_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89918.1"
                     /translation="MNAALMLTKIRQMVRSFGLPRIIIALFLFSLFILAPFFNVNVAT
                     SLSDTLNRFGMNAVLVLAMVPMIHSGCGLNFGLPLGIISGLLGATISLQVDVAGPASF
                     WLAILFATPFALILGWGYGQLLNRVKGGEMMIATYVGFSSVAFMCIMWLLLPFNNPKM
                     VWGFAGSGLRTTISTEGYYLHVLNDFLKIRIGNHFIFPTGMILFFTVLAFAMWAFLHT
                     KTGTAMTAVGSNPVFARASGIDVDRIRTLSVVLSTWLGAIGILVYQQSFGFIQLYMGP
                     FYMALPAVSAILIGGASVNKASILNVIIGTFLFQGILTMTPSVMNAMIQTDMSEVIRI
                     VASNGMILYALTRKTEAAR"
     gene            complement(2917..4542)
                     /locus_tag="PLF67_07645"
     CDS             complement(2917..4542)
                     /locus_tag="PLF67_07645"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE89919.1"
                     /translation="MNNELGVPLLEMQHISKDYFGNEVLSDINFSLRRGEILGLVGEN
                     GAGKSTLMKILFGMDEIHQTGGYGGSIFIEGDEVRFTSPIDALASGIGMVHQEFSLLP
                     DSTATENILLNREPLKHTLASELFGERLNTLNRESMEKIAQGAIDKLGVPLDGKMLVS
                     EMPVGHKQFTEIARELSKEATTEMEEGLRLLVLDEPTAVLSEQEAESLLKAMRTLSSE
                     GIAIIFISHRLQEIIDVCDTIMVMRDGRIIKTVPSEGVNVTDIAHWMVGREVNAAKRN
                     TRTFNYEDSKVVLSIRNLWVDMPGETVRNVNLDVYDGEILGIGGMAGQGKLGIPNGVM
                     GLFPAGGQIVFQGEELPLNQPRSLLNAGVAFVSEDRRGVGLLLDESLEWNITFTAMQI
                     QDKFLKRYLNGLVSWRDQKAIEEETMKYVKQLEIRCVSTRQNARELSGGNQQKVCLAK
                     AFAVTPKLLFVSEPTRGIDIGAKSLVLDALRRYNQESGTTIVMISSELEELRSVCDRI
                     AIVNDGKVFGILPATSDSAKFGLMMVGQEVQEV"
     gene            complement(4661..5872)
                     /locus_tag="PLF67_07650"
     CDS             complement(4661..5872)
                     /locus_tag="PLF67_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOE89920.1"
                     /translation="MKKTLAIAMVVLFAVSMVFAAGSKETASAKSTAFHVGIVTGTVS
                     QSEDDLRGAEKLIEEYGDVKSGGIINHVTYPDNFMEEQETTISVIAGLADDPLMKAVI
                     VNQAIPGTTEAFRIIKEKRPDIITIAGEPHEDPPVIQTAADLAVNNDFVSRGYLIIKT
                     AHDLGIKTFVHVSFPRHMSYETLSRRAAIMAETAKDLGMRFVYETAPDPVSDVGIAGA
                     QQYILEKVPAWLEQYGKDAAFFVTNDAHTEPLLKQLLAYGGYFIEADLPSPLMGYPGA
                     LGLDLSKEAGDFAAILAKVEKAVVDKGGAGRFGTWAYSYGYVTTAGLGTHAINVIKGE
                     SELTKLSDIMKAYGKYTGSAKWNGAFYNDVTTGVVARNHVLIYQDTYMMGKGYMGAAD
                     VVVPEKYFSIK"
     gene            complement(5986..6732)
                     /gene="rlmB"
                     /locus_tag="PLF67_07655"
     CDS             complement(5986..6732)
                     /gene="rlmB"
                     /locus_tag="PLF67_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOE89921.1"
                     /translation="MSDAIIGFHAIEEGLKNAPGGSVLYIAREMCSKAEVLENLARMN
                     RKTIVRKVTGTELDRLAGSSNHRGALLVLGSRSKSSSASRQLTVQEFCSNLKEEEGAL
                     VLILDGITDPHNLGAILRSADQFSVSLVIIPSRRSAQSNQTVVKVSSGAAHYVNQAVV
                     TNLNREIEYLKSQGFWVYGAALGGESLHETTFPKRTVLVLGSEGRGISRLTEKLCDHI
                     VAIPTTGNIDSLNVSVAAGIFLYEIRRQQA"
     gene            complement(6735..7859)
                     /gene="dnaJ"
                     /locus_tag="PLF67_07660"
     CDS             complement(6735..7859)
                     /gene="dnaJ"
                     /locus_tag="PLF67_07660"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOE89922.1"
                     /translation="MAKRDYYEVLGVEKTATLDEIKKAYRKLAIANHPDRNQGDKAAE
                     DRFKEATEAYDVLSDEKKRQMYDQFGFAGVDGGSGPRDYSNVYRDFSDIFGSGFGGGF
                     EDIFSSFFGGGGRASRAHRGPEPGASLRYDIEIDFKDAIFGTKVEISYSHQVACDVCG
                     GSGSESSAGMKTCPTCHGSGQVSRGGGFFTIASTCPTCSGNGQIIENPCQGCHGTGLK
                     RKQQKVKVTVPAGVDTGSRVVLRGMGDAGPNGGASGDLYVYINVRPHKYFIRQGDNLF
                     VQIPISITQAALGAEIMVPTIDGKKIKLSIPSGTQHGKMLRLRQYGATRLNSTDRGDM
                     YVKIDIQVPKRLNSKAKKLMQELSDALGEVTEPNPVSFED"
     gene            complement(7938..9851)
                     /gene="dnaK"
                     /locus_tag="PLF67_07665"
     CDS             complement(7938..9851)
                     /gene="dnaK"
                     /locus_tag="PLF67_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOE89923.1"
                     /translation="MGRIIGIDLGTTNSCVAVMEGTDSVVISNSEGQRTTPSVVAFTS
                     KGDRLVGQPAKNQMVTNAENTVYSIKRFMGRKYKDIPSEVNLIPYKLKEAAGGEIQID
                     IRGNSYSPPEISAAILQKMKKTAEDYLGETITEAVITVPAYFNDAQRQATKDAGRIAG
                     LDVKRIVNEPTAAALAYGLGKDASREERIAVYDLGGGTFDISILELGDGVFEVKSTNG
                     DTHLGGDDFDQRIISWMIDEFKKSATIDLSKDKMALQRLREAAEKAKIELSNSTSTDI
                     NLPFITADASGPKHLQMTLSRAKFEQLIADLIDRSRKPVQNALRDAGLSAGDIDEVIL
                     VGGSTRIPAVQAMVKELFGKEPHKGVNPDEVVAMGAAIQGGILGGKVKDVLLLDVTPL
                     SLGIETLGGVNTRLIERNTTIPTRKSQIFSTAADNQTAVSIHVLQGEREMASQNRTLG
                     NFDLVGIPSAPRGVPQIEVTFDIDANGIVHVSAKDLGTGKEQKIRIESSSGLSEAEIE
                     RMVKEAELHAEEDKRERARIDARNEADSMVYSTEKSLSEHGDKIEAADRAKIQSALDD
                     VKAALANANATADELKSKTEALQQASYKLAEEMYKQTQAEAGGPQTETESTAGSSSDT
                     VKNAEDADFEVVD"
     gene            complement(9926..10069)
                     /gene="grpE"
                     /locus_tag="PLF67_07670"
     CDS             complement(9926..10069)
                     /gene="grpE"
                     /locus_tag="PLF67_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOE89924.1"
                     /translation="MRKHGAYQVVEDENLEHETVLEEYATGYKLHGRVLRPSKVKVGR
                     PAI"
BASE COUNT         2157 a         2904 c         2668 g         2381 t
ORIGIN      
        1 cgattgccgg acataaaaag aggccgtcgc tttttcgact tttgcgacgg ccccttgatt
       61 cccctcgcgt ttcaatttcg ccttgaggta cttccagtag tttcgattct tttccgggtc
      121 gtcttcgttg cggagcacac cgatgatatc caagacggag aaccaccatc cggcgttccg
      181 ttcgtcccaa acggctctca tttcacggtc atccagcaat cggattgaaa tcttgctcat
      241 gctgttccca ttgtaccatg aaagccttag gaatcagaag gaatcgaagc cggcaagctc
      301 atcgaagacg acatcctctt cctgcggagc atcgctctga gcgacggcaa gcccctcgaa
      361 gttttcgagc cagaaggaaa cgtcttcggg gttcttcatg agtgccggca gactgatgag
      421 aaccatgttc tgcatcaggg gaatcctgaa ggaaagctca tggacgtgct cattccagga
      481 agcatcggtg tagacgacct tcaccgtgtg tcgttgcccg acgacgacgg ctttatcccg
      541 gattcgcggc gtcatctcca agacttcacc cttttttcct acctgaactt cgacaagctt
      601 cagcgcctga agctccaccc cgtctctggt gacggtgttg ttgtcgatca gaacggtgtg
      661 ctgacggccg atgtgaaaga gaaacacggc gaagaggaga atcaggatga tcgctccaac
      721 tcggatcaga agggttcgtt ggcgtttcgt catgacgtca cctcctggtc ctgggccaag
      781 gcaaggccgc tcatcccccg ctcccgcttc tttcggactt cataaagaac cagactggtg
      841 gtgatgaccc cgtaggaaac gaaaacccgg aagtattcgc cgagctgggc ctggccgatg
      901 aggcgctttc ccgccatcgg gctgacgatg aacatcagat ggaagagcac gacgcccaaa
      961 aagacgtttc ggatcttcgc actggcgacg gtggctccgc cgaccagaag agcggcgacg
     1021 ctgaacatcc cgatctgcat gtggctgtta taggtgttga gcgttccgat gttgtgcagg
     1081 tagatgatca ttccatatcc ggcaagaacc gtggagatga tgatgctcat gatccgggtg
     1141 tgctcaaccc ggattcccgc cgcccgggcg acttccatat cctggccgac cgctcgcatg
     1201 tcctgtccca gcttcgtctt cttgaaccaa aggatgaaga cgctgagcag cgcaatgaca
     1261 aggaaggttc ccaccggaat cggcacgccg ccgatccgga tcatcaaaaa gttgtccaac
     1321 gacttgcgga tgccgatcag gttcacggta ttccggattc cgtacccgcg gggcagcaac
     1381 aggttcggtg aaatgatctt gatcaacgga cccattccat agaggacgac cagctgatag
     1441 atgccgttca tgaagaaacc gatgatatag ctggtcacca tctcccggcc tttggccatg
     1501 ttcagaatct ttccgcacca ccacccgagc agaatggaaa gcggggtgga gacgatcatc
     1561 gccaaaagga ttcccggaat tccgacgatc ccccagtcgg tggtgaagat caggccgatc
     1621 tgacccgcca tcgctccgag ggtcatcccg aagttcaacc ccattccggc gaggatcggg
     1681 atgagaagtg aaacgatcaa aaaggtgttc cggctgattc gactgatcac ctctccggcc
     1741 agataagtcg gtgaatatcc gctcaaggga accccgaagg cgatgatgac cacgaaaaga
     1801 agcggtacgg tattgtttgc catgaagcgg aggattttct gtttcgtact caattctctc
     1861 atcgggccgc ctccgttttt ctcgtcagag cgtagaggat cataccgttg gaggccacga
     1921 tcctgatcac ttcgctcata tcggtctgaa tcatcgcgtt catcacgctg ggagtcatcg
     1981 tgagaatccc ttggaagagg aatgttccga tgatgacgtt gagaatcgaa gccttgttca
     2041 ccgatgctcc tccgatgaga atcgccgata ccgcaggaag agccatgtag aacggtccca
     2101 tgtagagctg gatgaacccg aagctctgct ggtaaaccag aattccgatc gcccccaacc
     2161 acgttgaaag cactacgctg agggttcgaa tccgatcgac atcgattcct gaagcccggg
     2221 cgaaaaccgg gttggatccg acggcggtca tcgccgtgcc ggtcttggtg tgcaggaacg
     2281 cccacatggc aaacgccaaa acagtaaaga acaagatcat tccggtggga aagatgaagt
     2341 gattaccgat tcgaatcttg agaaaatcat tcagcacgtg gaggtagtac ccctcggttg
     2401 aaatcgtggt ccgcaatccc gagccggcaa aaccccagac catcttcggg ttgttgaacg
     2461 gcaaaagaag ccacatgatg cacatgaatg cgaccgagga gaatccgacg taggtcgcga
     2521 tcatcatctc tccgcctttc acccggttga gcaattgccc gtacccccac ccaagaataa
     2581 gggcgaaggg cgtggcaaaa aggatcgcca gccaaaacga ggcgggaccg gcgacatcca
     2641 cctgaagact gatcgttgca cctaagagac cggagatgat ccccagcgga agaccgaagt
     2701 tgaggccgca gccgctatga atcatcggaa ccatcgccaa taccagcacc gcattcatgc
     2761 cgaagcggtt caaggtatcg gaaagacttg ttgccacgtt tacattgaag aacggagcca
     2821 aaataaacaa tgaaaacaaa aagagggcga tgatgattct tggcaatcca aacgaacgaa
     2881 ccatctgccg gatttttgtc agcatgagag cggcattcat acttcttgca cctcctgacc
     2941 aaccatcatc aaaccgaact tggcgctgtc actcgtcgcc gggaggatcc cgaacacctt
     3001 cccgtcgttg acgatcgcga tccggtcgca gacggagcgc agctcttcaa gctcgctgct
     3061 gatcatcacg atcgtcgtcc cgctttcctg gttgtaccgc ctgagggcat ccaggacgag
     3121 actcttcgcc ccgatatcga ttccccgagt cggctcgctg acaaacagaa gcttcggcgt
     3181 tacggcgaac gctttcgcca gacatacttt ctgctggttt ccaccgctga gctcacgggc
     3241 gttctgccgg gtggagacgc atcggatctc aagctgcttt acgtacttca tcgtttcctc
     3301 ttcgatcgct ttctgatcgc gccaggaaac aagtccgttg aggtagcgct tgaggaactt
     3361 gtcttgaatc tgcatcgctg tgaaggtgat attccattcg agggactcgt ccaagagcaa
     3421 tcccaccccc cggcgatcct cgctgacgaa cgcgacgccg gcgttcaaaa gcgaccgcgg
     3481 ctgattgagg ggaagctcct ctccttgaaa aacgatctgc cccccggcgg gaaagagacc
     3541 catcactccg ttgggaatgc cgagcttccc ttgtcccgcc attcctccga tgccgaggat
     3601 ctctccgtca tatacgtcaa gattgacgtt tcgaaccgtc tctccgggca tgtcgaccca
     3661 gaggttcctg atgctcagga caaccttcga gtcttcgtag ttgaaagttc gggtatttcg
     3721 ttttgcggcg ttgacctctc gacctaccat ccagtgggcg atatccgtaa cgttgacccc
     3781 ttcggaagga accgtcttga taatccggcc gtcgcgcatc accatgatcg tgtcgcagac
     3841 atcgatgatc tcttgcagac ggtggctgat gaagatgatc gcaatcccct cggaactgag
     3901 ggtgcgcatc gccttcagaa ggctttccgc ttcctgttcg ctgagtaccg cggtcggttc
     3961 atcgagaacc agaagtcgaa gcccctcctc catttcggtc gtcgcttcct tgctgagctc
     4021 ccgggcgatc tcggtaaact gtttgtggcc gacgggcatc tccgaaacga gcatcttccc
     4081 gtccaaagga acacccagct tgtcgatcgc tccttgagcg atcttctcca ttgactcccg
     4141 gttaagcgtg ttcaaccgct cgccgaagag ttcgctggct aatgtatgtt tcaacggctc
     4201 ccgattgagc aaaatattct ccgttgccgt tgaatccggc agcagtgaga actcctggtg
     4261 aaccattccg ataccactgg caagagcatc gatcggcgag gtgaaacgca cttcgtcccc
     4321 ctcgatgaag atcgatcctc catatccacc cgtctggtgg atctcatcca tgccgaagag
     4381 aatcttcatc aaagttgatt tgccggctcc gttttcccct acaagtccca aaatctcccc
     4441 gcgtctgaga gagaaattga tatcggaaag aacttcattg ccgaagtaat ctttgctgat
     4501 gtgttgcatc tccaaaagag gcaccccaag ttcgttattc atgatgtttc cttaactatc
     4561 catggatgtg accaatacct cgaaaaggga ttgatcccag ccacaagacg cccaaaaggg
     4621 ggagagtgac ctctccccca tgcaccgggc gctttggggc ttatttgatg gagaagtatt
     4681 tctccgggac gactacgtcc gcagctccca tatacccttt acccatcata taggtgtcct
     4741 ggtagatcaa cacgtggttg cgggcgacga caccggtggt cacatcgttg tagaatgcac
     4801 cgttccactt ggcgctgccg gtgtacttgc catatgcctt catgatgtcg ctgagcttgg
     4861 tcaattcgct ttcgcccttg atgacgttga tcgcatgggt tccaagtccg gcggtggtaa
     4921 cgtatccata ggagtacgcc cacgttccga agcgaccggc gccacccttg tcgacgacgg
     4981 ccttctcgac ctttgcgaga atcgcggcga agtcgccggc ctctttggag aggtcaagac
     5041 ccagtgctcc gggatagccc atcagcgggc tgggcaggtc ggcttcgatg aagtatccgc
     5101 cgtacgccag gagctgcttc agaagcggct cggtgtgggc atcgttcgtc acgaagaacg
     5161 ctgcgtcctt gccgtactgt tcgagccagg cgggaacctt ctcaagaatg tactgctggg
     5221 caccggcgat tccgacatcg cttaccgggt cgggagcggt ctcatagacg aagcgcatac
     5281 cgagatcctt ggcggtctcg gccatgatcg cagcccggcg gctgagggtt tcatagctca
     5341 tgtggcgggg gaacgagacg tggacgaacg tcttgatgcc cagatcatga gcggtcttga
     5401 tgatcaggta tccgcgggaa acgaagtcgt tgttcaccgc aagatcggcg gcggtctgaa
     5461 tcaccggcgg atcttcgtgc ggctctccgg cgatcgtgat gatgtcgggg cgcttctcct
     5521 tgatgatccg gaacgcctcg gtggtgccgg gaatcgcctg attgacgata accgctttca
     5581 ttaacgggtc gtccgccaga ccggcgatga cgctgatcgt cgtctcctgt tcctccatga
     5641 agttgtccgg ataggtaacg tggttgatga ttccaccact ctttacatca ccatattctt
     5701 caatgagttt ttccgcaccg cgcaggtcgt cttccgattg agaaaccgta ccggtcacga
     5761 ttccgacgtg gaatgccgta ctcttggcgg aagcggtttc tttggaacct gcagcaaaga
     5821 ccatggaaac tgcaaacagc acgaccatgg caatagctaa tgtctttttc attgctactc
     5881 tcctttagcg ggatattttc cctatccgta cataatacgt aaaagcatcg atcgcggcaa
     5941 gaagagaaca cgggtttatg caagatatgt aaatatggcg tatatttatg cctgctggcg
     6001 tcgaatttcg tagagaaaga ttcccgcggc gacggaaaca ttcagtgaat cgatgttgcc
     6061 ggtcgtcgga atcgccacga tgtggtcgca cagcttctcc gtcaaccggc tgattcccct
     6121 tccctcactg cccaatacaa gaacggtgcg cttcggaaag gtcgtctcat gcaggctttc
     6181 gccgccaagt gccgctccat acacccagaa cccctgactt ttcagatatt cgatctcgcg
     6241 attcagattc gtcaccaccg cctggtttac atagtgcgcc gcccccgagg agactttgac
     6301 caccgtctgg ttggattggg cgctccgcct gcttgggatg atgacaaggg agacggaaaa
     6361 ctggtcggcg gagcggagga tcgccccgag gttgtggggg tccgtgattc cgtcgaggat
     6421 cagcacgaga gcaccttcct cttccttcag gttcgagcag aactcctgaa ccgtcagctg
     6481 acgggaagcg gatgagcttt tacttcggga accgaggaca agaagagctc ctcgatgatt
     6541 ggaggagccg gcaagtcgat caagttcggt tccggttacc ttccgaacga tcgtttttcg
     6601 attcatccgc gccagatttt ccagcacctc ggctttcgag cacatctccc gggcgatata
     6661 gagaacggag ccgccggggg cgtttttaag cccctcttcg atcgcgtgaa agccgataat
     6721 cgcatcgctc atcatcaatc ctcaaaagaa accggattcg gttcggtgac ctcgccgagg
     6781 gcgtcactga gctcctgcat cagttttttt gctttagaat tcagccgttt aggaacttgg
     6841 atatcaattt tcacgtacat atccccccga tccgtcgaat tgagccgggt ggcgccgtac
     6901 tgacggagac ggagcatctt cccgtgctgg gttcccgacg ggatgctgag cttgatcttc
     6961 ttgccgtcga tcgtcggcac catgatctcc gctccgagag cggcctgagt gattgagatc
     7021 gggatctgga cgaagagatt gtctccttga cggatgaagt acttgtgcgg tctgacattg
     7081 atgtagacgt acagatcgcc gcttgctcct ccgttgggtc cggcgtctcc cattccccgc
     7141 agaaccaccc tgctgccggt atcgactccg gcgggaacgg tgaccttcac cttctgttgc
     7201 ttgcgcttca agccggtgcc gtgacacccc tggcagggat tctcgatgat ctgcccgttt
     7261 ccgctacagg tgggacaggt cgaggcgatc gtgaagaagc cgccgcctct gcttacctga
     7321 ccggagccgt ggcaggtggg acaggttttc atccctgccg aactttcact ccccgacccc
     7381 ccgcagacat cgcatgccac ttggtgggag taggagattt cgactttcgt tccaaaaatc
     7441 gcatctttaa aatcgatttc aatgtcatag cgaagggaag cacccggttc gggaccccga
     7501 tgggctcttg aggctcgtcc gcctcccccg aagaaggagg aaaaaatatc ctcaaacccg
     7561 ccaccgaagc cggaaccgaa gatatcgctg aagtcacgat agacgttgga ataatcacgc
     7621 gggcccgaac cgccatccac tcctgcaaag ccgaattggt cgtacatctg tcgcttcttt
     7681 tcatccgaaa ggacatcata cgcctcggtt gcttccttga agcggtcctc ggccgccttg
     7741 tcaccctgat tgcgatcggg gtgattggcg atcgcaagtt tgcgatatgc ttttttaatt
     7801 tcatccaagg tagcggtctt ctccacccca aggacctcat aataatctcg tttcgccaca
     7861 gtttcctcgt ctacaccatc atgtcggccg gagccgacat gatggtaatc aacgtattct
     7921 atcgtccttc aaggccgtta gtctacgacc tcgaagtcgg catcctcagc attttttacc
     7981 gtgtccgaag aagagcctgc cgtactttcc gtctcggtct gggggcctcc ggcttccgct
     8041 tgggtctgtt tatacatctc ttcagccagc ttgtaggaag cttgttgcag ggcttccgtc
     8101 ttgctcttca gctcatcggc agtagcattc gcgttggcca acgcggcttt cacgtcatcc
     8161 aaggcgcttt ggatcttcgc ccggtccgcc gcctcgatct tatctccatg ttcggagagg
     8221 gatttctcgg tggaatacac cattgaatcc gcttcattgc gggcatctat tcgagcccgt
     8281 tcccgcttgt cctcttcggc gtgcagttcc gcttccttga ccatccgctc gatctccgct
     8341 tcggataggc cgctggagga ttcgattcgg atcttttgct ccttgccggt tccaagatcc
     8401 tttgccgaaa cgtgaacgat tccgttcgca tcgatatcga aggtcacctc gatctgaggc
     8461 acaccccgcg gtgcggaggg aattccgacg aggtcaaagt tgccgagcgt cctgttctgg
     8521 gatgccatct cgcgctctcc ctgaaggacg tggatgctga ccgcggtctg attgtccgcg
     8581 gcggtggaga agatctgact cttgcgagtc gggatcgtcg tgttccgctc gatgaggcgg
     8641 gtatttacgc ctcccaacgt ctcgatcccc agagaaagcg gggtaacatc gagaagcagg
     8701 acatccttca cctttccgcc gaggattccg ccctggatcg ccgcacccat cgcaacgacc
     8761 tcatcggggt tcacaccctt gtgcggctct tttccgaaga gctccttgac catcgcctgc
     8821 acggcgggga ttctcgtcga tccgccaacg aggatgacct catcgatatc accggcgctc
     8881 aggccggcat cgcgaagggc gttctggacg ggctttctgg atcgatcgat cagatcggcg
     8941 atcaattgct cgaacttggc gcggctcaag gtcatctgca ggtgcttcgg tcccgatgca
     9001 tccgccgtga tgaacggcag gttgatatcg gtgctcgttg agttcgagag ctcgatcttc
     9061 gccttctccg ccgcctcgcg caggcgttga agcgccatct tgtccttcga gaggtcgatc
     9121 gtcgcgctct tcttgaactc atcgatcatc caggagatga tccgctggtc aaagtcgtca
     9181 ccgccgaggt gggtatcacc gttggtggac ttcacttcaa agactccgtc tccgagttca
     9241 aggattgaaa tatcgaacgt tccgccgccg aggtcataca ctgcgatgcg ctcttcacgg
     9301 ctggcgtctt tcccgaggcc ataggcaagg gctgcggcgg tcggttcgtt gacgatccgc
     9361 ttcacatcca agccggcgat tcgaccggca tccttggtcg cctgtcgttg agcgtcattg
     9421 aaataggcgg gaaccgtgat gaccgcttcg gtgatcgtct cgccaaggta atcttcggcg
     9481 gtcttcttca tcttctgcaa aatcgccgcg ctgatctccg gcggactgta ggagttgccg
     9541 cgaatatcga tctgaatctc gccccctgcg gcctccttca gcttgtaggg aatcaaattc
     9601 acctcggagg gaatatcctt atacttgcgc cccatgaagc gcttgatcga atacaccgta
     9661 ttttcggcgt tcgttaccat ctggttcttt gcgggttgac caacgagacg atcgcctttg
     9721 ctggtaaacg caaccacgct gggggttgtc cgctgtcctt cactgttgga aataacgacc
     9781 gaatcggttc cttccatcac tgcgacgcag ctattggtcg ttcccaagtc gatcccaata
     9841 attctaccca ttgtataact cctctttatc tcatcaattc tgtttcattc cgtgtatgta
     9901 aaatactcat acaccggcct tatcgtcaaa tggccggtcg tccaactttc acttttgagg
     9961 gacgaagcac tcgaccatga agtttatagc cggtggcata ctcctccagg accgtttcat
    10021 gttcaagatt ttcatcttca acgacctggt acgccccatg cttccgcagt aactaaccaa
    10081 aactaggtga gcgtagtttt ttatttgtaa
//
LOCUS       DAOSLI010000057         9530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_6908_length_9530_cov_27.248971, whole genome shotgun sequence.
ACCESSION   DAOSLI010000057 DAOSLI010000000
VERSION     DAOSLI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6908_length_9530_cov_27.248971"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..438)
                     /locus_tag="PLF67_07675"
     CDS             complement(88..438)
                     /locus_tag="PLF67_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HOE89925.1"
                     /translation="MTIICYPRCGTCKKAEAFLKEHNISYTYRDIKEANPTKEELASL
                     HERSGLPIRRFFNTSGQLYREHNLKDRLPTMSVDEALELLSTNGMLVKRPILIAGDRV
                     LVGFRDAEWKELIG"
     gene            604..1953
                     /gene="tig"
                     /locus_tag="PLF67_07680"
     CDS             604..1953
                     /gene="tig"
                     /locus_tag="PLF67_07680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOE89926.1"
                     /translation="MIANKSIKELDHSAVELTITVKSETIEKEYKSALAKYAKTLQIK
                     GFRKGKAPQSVVEAKLGQAVREESTFNVIEDALKEAIGGVEDKYKPLSYSTPQLLDEE
                     NLTPFEAEKDLTFSVKYDVMPNVELPAYKGLKISYPKVTLPKEAVDAEIEKLRQQNAM
                     TIEKKGKAADGDIVTVDYVEVAEDGSEVEETKRADFVFTLGSGSNFYEIDKDIVGMAA
                     GEEKTVEKTYGEDSDKAGLAGRTLSLKITLKALKMKDVPELDDEFAQDVKDEYKTVAD
                     LVKGTKEKLQEALDAKLESEKYKALYAELLKNTTIALPETMVTMELEQAWKRYVRQTG
                     LSEEQIVQLLSFQGQSKEDIIAPWREGAEESLKTQLILDKIKDEEKFEIDEEDLKKLA
                     DEQLAGITDEEQRSYYVSMMEDDMRFSKTGPFLLENNTFTEGEKVEYTTFMDPSYGM"
     gene            2009..2617
                     /locus_tag="PLF67_07685"
     CDS             2009..2617
                     /locus_tag="PLF67_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOE89927.1"
                     /translation="MNNHPARMYGPIPMVVEQSGIGERAYDIFSRLLKDRIIFLDGEI
                     HDAAADLVVAQLLFLESEDPKKDISLYINSSGGSVTAGLAIYDTMQYLHSDVQTICMG
                     QAASMAALLLSAGADGKRYILPSARVMIHQPWGGVQGQSSDISIHAKEIGRLKRLTTE
                     IIARHTKKSFEEVARDVERDFFLSAEEAQTYGIVDSIMTRRA"
     gene            2617..3846
                     /gene="clpX"
                     /locus_tag="PLF67_07690"
     CDS             2617..3846
                     /gene="clpX"
                     /locus_tag="PLF67_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOE89928.1"
                     /translation="MARASRVCSFCGKGQHEVRRLINGPGVAICDECVEVCSDMLTND
                     PSFVEDVVGVPETIPTPRELKEYMDEYVIGQDSAKRILAVAVHNHYKRVKFQKQIAEQ
                     GVELDKSNILMVGPTGTGKTLLASTLAKKLQVPFAIADATTLTEAGYVGEDVENILLK
                     LIQNANDNIKAAEYGIIFIDEIDKIAKKGENPSITRDVSGEGVQQALLKIIEGTIASV
                     PPQGGRKHPSQELLKINTKNILFICGGAFVGLDKVIEKRVSSHAMGFGAPIVNSQEKN
                     LLDLYNQLMPDDLIKFGLIPEFIGRLPIHAALDNLSKEDLKRIIVEPKNSIVRQYEAS
                     FRLDGIDFTFDDDAVAAVAEKAYEQKTGARGLRSIVENIMMEIMYEIPSIENVQKVIV
                     TGDCVINGTSPEVIRGA"
     gene            3836..4831
                     /locus_tag="PLF67_07695"
     CDS             3836..4831
                     /locus_tag="PLF67_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOE89929.1"
                     /translation="MAPDFFSFPPLPEEIRRVIEDGSSFLVIGHVSPDGDCLSSQITM
                     GALLENLGKQVALVNAGPFERSEITHLAGLFQTSITREMKERNPIVVVVDCSSIERIG
                     SLAEEIEGLTTIIFDHHASGEFFGDYRYITPRSISTTFIIMHLYKEFGVEITPEIADH
                     LFFGFATDSGFFRFIGSYRGDVLRYAAELVDLGVSPNEMADRISGGKNLDYIKYVGKL
                     IDRSEVLLDGRLIIATSYIRDSRTFDTTDRPSDMLYSMLLAIKGVEVVLYLKELEPQL
                     TEIGFRSSHVSPVDVGELAKRLGGGGHRRAAGVTVNESVESIRNRLILLVKELLR"
     gene            4883..5878
                     /locus_tag="PLF67_07700"
     CDS             4883..5878
                     /locus_tag="PLF67_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89930.1"
                     /translation="MALYPMSEAASLELTRRVVTYQHTGDPALHAELKGEIMELLYTI
                     PSTLRILDSEDCCEFLFYCLSAIDNLIAGYEEGKLSFVGFLTEVVRRRVRYFLCEKQK
                     NEQKLRLLHQSEYFFPETDDIGFESAEPPIIYTFKGLPTVNIASLPSLFAELLRKRTP
                     STYPIRKELEELKTVLTKGVNRRRMLIILTITPSLYAHHLLEELSSLLLFDLTDLCRY
                     LNTASLALHSKETIKQDFEQTCTRHFRRLLEIEQALEDESDEVKKRQLIALRDWTQEK
                     YRKKIAKLRSMEQSLSHRQVGELLHIPKGTVSSSVFHIKKLLYTYIDERGVNDYP"
     gene            5882..6472
                     /locus_tag="PLF67_07705"
     CDS             5882..6472
                     /locus_tag="PLF67_07705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOE89931.1"
                     /translation="MDILLASNNEHKKEEFARLLAPHRIVMPKDLGIVFDVEEDRPSF
                     TENALLKAATLKVLAPSYWVLGDDSGLCVDALGGGPGLQTARYGFEVHNHLMDSKERN
                     AYLLEHMEGVENRSARFVCALALMVSDYRVFTVCEEAKGSIARHERGSEGFGYDPIFL
                     VEGKDLTMAELGMEMKDELGHRGKASRMLMELVRRV"
     gene            6474..8252
                     /gene="pepF"
                     /locus_tag="PLF67_07710"
     CDS             6474..8252
                     /gene="pepF"
                     /locus_tag="PLF67_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOE89932.1"
                     /translation="MQVLTRSEQKTMDTWDLGSLFENDAAWEAAVEELRSFIKEIPSY
                     KGSLKAGRESLLKTMAFHAKVGILAERVHSYAFLNWASDTSNPANVERSSIASTLMTE
                     LGAALAFFDPELLSIDEEVMNGYLADEAFEPYKVFFMRSMRYKAHVLSEREERIMALQ
                     RESAQTPRNAFSDLTNIDFDFGRIGDKPLTQSTFSSFLMSEDRALRKKAYKQFYSRYN
                     RNRHTLFRLYEGQVKQDIFRHTVRSYPSSREMSLFADDVPTGVYDRLIESVHAGLPVL
                     HRYYELRKKILGVRNLAHWDVYVPLVSGVKAHTPYEEAVKIIGEALKPLGSEYVDTLT
                     RGLTIERWVDRYENKGKRSGAFSSGSYTGYPYILMNYKEDVLRDLFTLAHEGGHSMHS
                     YYSARNNPFHHWDYSIFEAEVASTFNEQLVANYMIESTDDVQTKRYIIAKQLDDVVAT
                     LFRQTMFAEFEHKIHKKAEASIPLTLDLVRSTYGDLLRQYFGPGVTFIDESNLEGLRI
                     PHFYSPFYVYKYATGLSAAIALSKEVLGGGEEERARYLRFLSNGGRYFPIENLKQAGV
                     DMSTGKPVDDAIAHFAQRLELFETLS"
     gene            8322..8405
                     /locus_tag="PLF67_07715"
     tRNA            8322..8405
                     /locus_tag="PLF67_07715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8356..8358,aa:Leu,seq:gag)
     gene            complement(8468..9340)
                     /locus_tag="PLF67_07720"
     CDS             complement(8468..9340)
                     /locus_tag="PLF67_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89933.1"
                     /translation="MKKSKIVLIAVLTVAVVLIFSGCGKKQEAPAFSASPAPVAATAA
                     AAPKAALADGNYFAIDRSEAAPNWRYYVELAVSGGAITEVNWSAVNPAGMDKKTVDKA
                     GQYNMVKYGGAQAEWYQQAEKVEEQLIKTQSADLPDAIGGVSIGFDEFKALVKQALAS
                     PPAVRGPYADGEYYAEEGAFGSSGWKYFVSLLVRNGNIVHANWSAVDKDGRDKRQVAA
                     SGEYGMIKVSSLKKEWHEQALEAEMYLLKTQDPKQIALKADGKTDAIAGATITVGVFF
                     DLAEAALANGPRTY"
BASE COUNT         2312 a         2580 c         2560 g         2078 t
ORIGIN      
        1 agagctccac cttgccaagg tggatgtcgc gggttcaagt cccgtctttc gctctaggca
       61 gtcggagaaa tccggctgcc tgtttttcta tccgatgagc tctttccact ccgcgtccct
      121 gaaacccacg agcacccgat caccggcaat gagaatcggc cgtttcacca gcatgccgtt
      181 ggttgaaagc agttcaaggg cttcatcgac gctcatcgtc ggaagacggt ctttaagatt
      241 atgctcccga tagagttgac cgctggtgtt gaaaaagcgc cgaatcggaa gaccgcttcg
      301 ttcatggagt gaagcaagct cttctttcgt cggattcgct tccttgatgt cacgataagt
      361 gtaggaaata ttgtgttctt tcagaaatgc ttccgccttt ttacatgtac cgcaacgggg
      421 gtaacagata attgtcatat gatcctcctt gtccatcgta gcagaagcag cgctgcctcg
      481 atagtcactg cttccgtata tttaccctag accaaagcta aaaaagttgt tatatacaat
      541 atgtttccac caagtgagct tggtggctgt aaactccgcc tacaggcaaa aggatgaagt
      601 agaatgatcg ccaataagag tatcaaggaa ttggaccact cagccgtcga actgactatc
      661 accgtgaagt cggaaacgat tgagaaggaa tacaagagtg cattagcgaa atatgcaaag
      721 acgctgcaga tcaagggatt ccgaaaagga aaagctcctc agtcggttgt cgaagcaaag
      781 cttggccaag cggttcggga agagtccacc tttaatgtca tcgaagatgc tcttaaagag
      841 gcgatcggcg gagtcgagga caagtacaag cccctttcct actccacccc tcagctgctg
      901 gatgaagaga atctcactcc cttcgaagcg gagaaggatc tcaccttcag cgtgaaatat
      961 gatgtgatgc cgaatgtgga actgcccgcc tataagggat tgaaaatctc ctatcccaag
     1021 gtaaccctcc cgaaggaagc ggtcgatgcc gagatcgaga agctccgcca gcaaaacgca
     1081 atgacgatcg aaaagaaggg caaagcggct gacggtgaca ttgtcacggt ggactatgtc
     1141 gaggtcgccg aggacggcag cgaggtcgag gagaccaaac gcgccgactt cgttttcacc
     1201 ctcggttcag gctcgaactt ctatgagatc gacaaggata tcgtggggat ggcggcgggc
     1261 gaggagaaga ccgtagaaaa gacctacggc gaagacagcg acaaagccgg cctggccggt
     1321 cgaaccctct cgttgaagat caccttgaaa gccctgaaga tgaaggacgt ccctgaactc
     1381 gatgacgagt tcgcccagga tgtcaaagat gagtacaaaa ccgtggccga cctggtcaag
     1441 ggaacgaaag agaagcttca ggaagcgttg gacgccaagc tcgagagcga gaaatacaag
     1501 gccctctacg ccgaactgct gaagaacacg acgatcgccc tcccggagac gatggtgacg
     1561 atggagcttg agcaggcttg gaagcggtat gtccggcaaa ccggtctctc cgaagaacag
     1621 atcgtccaat tgctctcctt ccagggtcag agcaaggaag acatcatcgc tccctggcgc
     1681 gaaggagcgg aggagtcctt aaagacccaa ctgattcttg ataagatcaa ggatgaggaa
     1741 aagttcgaaa tcgatgagga agatctgaaa aagcttgccg acgagcagct tgccggaatc
     1801 accgatgaag agcagcgctc ctactatgta tcgatgatgg aagatgatat gcgcttctcc
     1861 aagaccggac cgttcctgct tgagaacaac accttcaccg aaggtgagaa ggttgagtat
     1921 actacgttca tggatccttc ctacggcatg tagagggaag cttgaatgcg atggatgaca
     1981 cggttcttgt catacaggag gattatagat gaataatcat cccgcgagga tgtatggccc
     2041 gattcccatg gttgtcgagc aatcaggaat tggtgaacgg gcatatgata ttttcagccg
     2101 cctgctgaaa gaccgaatta ttttcctgga cggagagatt catgatgccg cggcggatct
     2161 cgtcgtggcc cagctgctct ttctggaatc ggaggatccc aagaaggata tcagcctcta
     2221 catcaacagc tcaggcggtt cggtaaccgc gggtctggcg atctacgata cgatgcaata
     2281 ccttcacagc gacgttcaga cgatctgcat gggccaagct gcgagcatgg ccgctctgct
     2341 gttgtcggcg ggagcggatg gcaagcgtta tatcctccca tccgcccggg tgatgattca
     2401 ccagccctgg ggtggagtgc aaggtcaaag cagtgatatt tccattcacg caaaagagat
     2461 cggccggctc aagcgcctta ctaccgagat catcgcccgt cacacgaaga agagctttga
     2521 agaagtcgcc cgggatgtcg agcgggactt cttcctctca gcggaagagg ctcagacgta
     2581 cggtatcgtg gactcgatca tgacgaggag agcgtaatgg ccagagcttc cagagtctgt
     2641 tccttctgcg gcaaaggcca acacgaggtt cgccgcctga tcaacggccc cggcgtcgct
     2701 atctgtgacg agtgcgtcga ggtgtgcagc gacatgctca caaacgaccc ctcattcgtt
     2761 gaagacgtgg tgggagttcc cgagacgatc cccactccgc gggagctgaa ggagtacatg
     2821 gacgagtatg tgatcggtca ggattcggcg aaacggatct tggcggtcgc tgtacacaat
     2881 cactataagc gggtgaagtt ccagaagcag atcgccgagc agggcgtcga gttggacaag
     2941 tcgaacattc tcatggtcgg acctacggga acgggaaaaa cccttctcgc ttccacactg
     3001 gccaagaagc ttcaggttcc cttcgcgatc gccgatgcga cgacgctcac cgaagcggga
     3061 tatgtcggcg aagacgtcga gaatattctc ttgaaactga ttcaaaacgc caacgacaat
     3121 atcaaagccg ccgaatatgg aatcatcttc atcgatgaga tcgacaagat cgccaagaag
     3181 ggagaaaacc cctcgatcac ccgagatgtc tccggtgaag gagtccagca ggcgctcttg
     3241 aagatcatcg agggaacgat cgcctcggtt cccccgcagg ggggacgaaa acatcccagt
     3301 caggagcttt tgaagatcaa cacgaagaac attctgttca tctgcggcgg agcgttcgtc
     3361 ggattggaca aagtgattga aaagcgggtc tcatcccatg cgatgggttt cggcgctccg
     3421 atcgtcaaca gtcaggagaa gaatctcctt gatctgtaca accagctgat gccggatgat
     3481 ctgatcaagt tcggcctgat ccccgagttc atcggacgcc tgccgatcca tgcagcgttg
     3541 gacaacctct caaaggagga tctgaagcgg atcatcgtcg aaccgaagaa ctcgatcgtt
     3601 cgccaatacg aggcttcctt ccgccttgac gggatcgact ttacttttga tgatgatgcg
     3661 gtggcggcgg ttgccgagaa agcatacgaa caaaagaccg gagcccgggg gctgcgctcg
     3721 atcgtcgaga acatcatgat ggagatcatg tacgagattc cttcgattga aaacgtccag
     3781 aaggtcatcg tgaccggcga ttgcgtcatc aacggcacca gtccggaggt aattcgtggc
     3841 gcctgatttt ttctcgtttc ctcctctgcc cgaagaaatc cgaagagtca tcgaggatgg
     3901 atcctccttc ctcgtcatcg gccatgtcag tcccgacggg gactgtctct cctcccagat
     3961 cacgatggga gcattgctgg aaaatcttgg caaacaggtg gcgctggtga acgccggtcc
     4021 gtttgagcgg agcgagataa cccacctcgc cggcctcttc caaacctcga ttacccggga
     4081 gatgaaagag cggaatccca tcgtggtggt cgtcgactgc tcttcgattg aacggatcgg
     4141 ttccctggcg gaagagatcg agggtttgac caccatcatc ttcgatcatc acgccagcgg
     4201 cgagttcttc ggtgattacc gctacatcac tccccgttca atctccacaa cgttcatcat
     4261 catgcacctc tacaaggagt tcggtgttga aatcacccca gagatcgccg atcatctctt
     4321 cttcgggttt gccaccgaca gcggattctt ccgcttcatc ggttcctatc gcggtgatgt
     4381 tctgcgctat gccgccgaat tggtcgactt aggtgtctcg ccaaatgaaa tggcggacag
     4441 gataagcggc ggaaaaaacc tcgattacat aaagtacgtc ggtaaattga tcgaccgcag
     4501 cgaggttctg cttgacggac gcctgatcat cgctacctcc tatatccggg acagtcgaac
     4561 gttcgatacg accgatcgcc ccagtgacat gctctattcg atgcttctgg cgataaaggg
     4621 tgtcgaggtg gtcctctact tgaaggaact tgaaccgcaa ttgaccgaga tcggattccg
     4681 atccagtcat gtaagcccgg tggatgtcgg cgagctggcc aaacggcttg gtggaggcgg
     4741 acataggcga gccgcggggg tgacggtgaa cgagagtgtc gaatcgatcc gcaatcgcct
     4801 cattcttctg gtaaaggagc tccttcgcta aacttggcat acacattgca tgatagagtg
     4861 catcccccgt aaggagcact gtatggcatt gtatccgatg agtgaagccg catccctgga
     4921 actcacccgg cgggtagtca cctaccagca taccggtgac ccggccctcc atgccgagct
     4981 caagggagag atcatggagc tcctctatac gattccctcg acgctgagga ttctggactc
     5041 ggaggattgc tgtgaattcc tcttctactg tctttcagcg atcgacaacc tgattgccgg
     5101 ctacgaggag ggaaaactct cctttgtcgg cttcctgacc gaagtggttc gaagacgggt
     5161 ccgctacttt ctctgtgaaa aacaaaaaaa tgaacagaag ctccgcctgc ttcatcaatc
     5221 ggaatacttc tttccggaaa ccgatgacat cggctttgaa agcgccgaac cgcccatcat
     5281 ctataccttc aaagggttgc cgaccgtcaa catcgcttcc ctgccttccc tgttcgccga
     5341 gcttctgaga aaacggaccc cctcgacata tccgatcaga aaagaactgg aagagcttaa
     5401 aaccgtcttg accaaagggg tgaatcgaag acggatgctc atcatcctca ccatcacccc
     5461 ctctttatat gcccaccacc tccttgaaga gctgagctcg ctgttgttgt tcgatctgac
     5521 cgatttgtgt cgatacctca acacagcgag tttggctctt cattcaaagg aaacgataaa
     5581 acaggatttc gaacaaacat gcacccggca cttccgccgc ctcttggaga ttgagcaggc
     5641 gcttgaagat gaaagcgatg aagtgaagaa gcgacagttg atcgcccttc gcgattggac
     5701 tcaggaaaag tatcggaaaa agatcgccaa actccgctcc atggaacaaa gtctctccca
     5761 ccgtcaggtg ggtgaactgc ttcatattcc caaagggacg gtctcctcct cggtgtttca
     5821 catcaaaaaa cttttataca cctatataga cgaaagaggt gtcaatgact atccttagtt
     5881 aatggatatt ctgcttgcgt ccaacaatga acataaaaag gaagagttcg ctcgattgct
     5941 tgccccccat cgcatcgtga tgccgaagga tctgggtatt gtgttcgatg tcgaggagga
     6001 caggccgtcc ttcacggaaa acgccctgct caaggcagcc actctgaagg tccttgcgcc
     6061 ctcctactgg gtcctgggtg atgattcggg attatgcgtc gacgcgctgg gcggaggccc
     6121 cgggctccag accgcccgct acggctttga agtccacaac catctcatgg acagcaaaga
     6181 acgcaacgcc tacctgctgg agcatatgga aggcgtcgag aatcgaagcg cccggtttgt
     6241 ctgcgccctc gccctgatgg tcagcgacta tcgggtgttc acggtgtgcg aagaagccaa
     6301 gggctcgatc gcccgccatg aacgcggaag tgaggggttc ggctacgatc cgatcttcct
     6361 cgtggaaggc aaagacctga cgatggccga actggggatg gagatgaagg atgagctggg
     6421 acatcgggga aaagcaagca gaatgcttat ggagctggta aggagagtat agcatgcagg
     6481 tgttaacaag aagtgaacaa aagacgatgg atacatggga tctcggttcc ctcttcgaga
     6541 atgatgcggc gtgggaggca gcggtcgaag aacttcgatc attcatcaaa gagatccctt
     6601 cctacaaagg cagcctgaaa gccggcaggg agtcgttgct caagacgatg gcgtttcatg
     6661 ccaaggtcgg gatcctcgcc gaacgggtgc attcctacgc gtttctcaac tgggcgagcg
     6721 acacctcgaa tccggccaac gtcgaacgct cctcgatcgc cagcaccttg atgaccgaac
     6781 tcggggcggc gcttgcgttc ttcgatcccg aactgctttc gatcgatgag gaggtgatga
     6841 acggctatct ggcggacgag gcgttcgagc cgtacaaggt ctttttcatg cggagcatgc
     6901 gctacaaggc ccacgtcctc agtgaacggg aagagcggat catggcgctc cagcgggaaa
     6961 gcgcgcagac gccgagaaac gcgttcagcg acctgacgaa catcgatttc gacttcggcc
     7021 ggatcggaga caagccgctg acccaatcca ccttctcctc ctttctgatg agcgaggatc
     7081 gggcgctcag gaagaaggcg tacaaacagt tctattccag atacaaccgg aaccgccaca
     7141 cccttttccg cctctacgag ggtcaggtga aacaggatat cttcaggcac accgttcgtt
     7201 cctatccatc cagccgggag atgtcgctct tcgccgatga cgtgccgacc ggcgtctacg
     7261 accggttgat cgagagcgtc catgcgggct tgccggtcct tcatcgctat tacgagctgc
     7321 gcaagaagat cctcggagtg cggaacctcg cccactggga cgtgtacgtc cccttggtca
     7381 gcggtgtcaa agcccatacg ccgtacgagg aggcggtgaa gatcatcggc gaggcgctca
     7441 agccgcttgg cagcgagtat gtcgacaccc tgacccgcgg tctcacgatc gagcggtggg
     7501 tcgaccgcta cgagaacaag gggaagcgca gcggcgcgtt ctcctccggc tcctacaccg
     7561 ggtaccccta catcctgatg aactacaagg aggatgtgct gcgcgatctc ttcacgctcg
     7621 cccatgaagg ggggcactcg atgcactcct actactcggc gcgcaacaac cccttccacc
     7681 actgggatta ctcgatcttc gaggcggagg tcgcctcgac gttcaacgag cagctggtcg
     7741 ccaactacat gatcgagtcg accgatgatg ttcagaccaa gcgctacatc atcgccaagc
     7801 agctggatga cgtggtcgcc accctcttca ggcagacgat gttcgccgag ttcgagcata
     7861 aaatccacaa gaaggcggaa gcttccatcc ccttgacgct cgatctggtt cgcagcacat
     7921 atggcgatct tctccgccaa tacttcgggc cgggggttac gttcatcgat gaaagcaatc
     7981 tggaaggttt gaggattcca cacttctact cccccttcta cgtctacaag tacgcaaccg
     8041 gcctcagtgc ggcgatagcg ctctccaaag aggtgcttgg aggaggtgaa gaggagcggg
     8101 cccgctatct gaggttcctt tccaacggcg gacggtattt cccgatcgaa aacctcaaac
     8161 aagcaggcgt ggacatgtcg accggcaagc cggttgatga cgcaatcgcc cacttcgccc
     8221 aacggctgga gctttttgaa acgctcagtt aggattcgga aaaagggctt gaccttcaat
     8281 tcgtcttcat tgtaaggttg ccctgttcaa ctaattcatg tgcggtagtg gtggaattgg
     8341 tagacacgct agcttgaggt gctagtggga gagatcttgt gctggttcga gtccagtcta
     8401 ccgcagatat ggcaaaacgc tccggaaccc ccggagcgtt cgtattgcaa agagctgatt
     8461 ttaaaaatca gtatgtcctc ggtccgttgg ccaacgccgc ttcagccaag tcgaagaaaa
     8521 cacctacagt gattgtggct ccggcgatcg cgtcggtctt tccgtccgcc ttcaatgcga
     8581 tttgcttcgg atcctgagtc ttcaacaggt acatttccgc ttccaacgct tgctcgtgcc
     8641 actctttttt gagagaactc accttgatca tcccatactc gccggaagcg gcaacctgac
     8701 gcttgtcccg accgtcttta tcaacggcgc tccagttcgc atgaacgatg tttccgttgc
     8761 ggacgagcaa cgaaacgaaa tacttccacc ccgaactccc gaaagcccct tcttcagcgt
     8821 aatactcacc gtccgcatac ggacccctga cggccggagg tgaagcgagg gcctgtttca
     8881 ccaatgcttt gaattcatcg aagccgatgg aaaccccgcc aattgcatcg ggaagatccg
     8941 ctgactgcgt tttgatcagc tgttcttcca ccttctcagc ctgctggtac cactccgcct
     9001 gagcgccgcc atacttgacc atgttgtact ggcccgcctt gtccaccgtc ttcttgtcca
     9061 tgcccgccgg attgaccgcc gaccagttca cctcggtgat ggcgccgccg gaaacagcca
     9121 actcgacata atagcgccaa ttcggggcgg cttcacttcg atcaatcgcg aagtagtttc
     9181 cgtccgccaa tgcggctttg ggtgccgccg ccgctgttgc tgccactgga gccggagaag
     9241 cggaaaacgc cggtgcttcc tgcttctttc cgcatccgct gaagatgagc accaccgcca
     9301 cggttaaaac ggcaatgaga acaatcttgc tctttttcat cgtatactcc tcgtatccat
     9361 gtttctcttt ttaatggtta ggatccctat acattatgat actattgtaa cgagaatttg
     9421 caatactatt aaaacactat gattttcccg ccaatccggt gaaaggcata taattctcta
     9481 tctataaata tgttatgttg gaaagacgat tggcatcaac catataaatt
//
LOCUS       DAOSLI010000058         9462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_6960_length_9462_cov_27.442649, whole genome shotgun sequence.
ACCESSION   DAOSLI010000058 DAOSLI010000000
VERSION     DAOSLI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6960_length_9462_cov_27.442649"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..660
                     /locus_tag="PLF67_07725"
     CDS             73..660
                     /locus_tag="PLF67_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOE89934.1"
                     /translation="MKKRHWALGAIVGVVLLALGIFLIFQEESFKKIFVSLLGVYMIG
                     SGFSTLLGLKSYQLGPRLRPATLIKALLTLLLGVIALILPILVADLSFLILLYIIATE
                     LLFSGIISIINLVAVRNLDIAFSPIIGDALISLILSMLLFFFPRQIGTVLLKGVGILV
                     IAIGLFFIIASLITRRTGKREEGKTIEGEAEILEP"
     gene            complement(657..935)
                     /locus_tag="PLF67_07730"
     CDS             complement(657..935)
                     /locus_tag="PLF67_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89935.1"
                     /translation="MRSCSHCSTTIDSTLSIGYATLCPTCSEPLHSCVNCRFYSPGSY
                     HDCAEHVEEFISDKNKANYCDSFILGSNHPTKKASDEARKKAEAIFSF"
     gene            complement(935..1339)
                     /locus_tag="PLF67_07735"
     CDS             complement(935..1339)
                     /locus_tag="PLF67_07735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOE89936.1"
                     /translation="METVFTKIRAGAIPSAKVYEDEICFVILDINPVNKGHLLIITNE
                     VHPTLADTPDEVLSHLLVIAKKADAKLREVLGCAATNVVINNGEASGQEIPHLHLHII
                     PRYADDGKRLTFPKAHYDEGEIAALGKRLEFE"
     gene            1525..3633
                     /gene="fusA"
                     /locus_tag="PLF67_07740"
     CDS             1525..3633
                     /gene="fusA"
                     /locus_tag="PLF67_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE89937.1"
                     /translation="MTDLRHLRNIGISAHIDSGKTTLSERILYYTNKIHEVHEVRGKD
                     GVGATMDSMELERERGITIASAATNVVWKGNEINVIDTPGHVDFTIEVERSLRVLDGA
                     ILVLCSVGGVQSQSITVDRQMKRYKVPRIAFVNKCDRTGANPYKVKNQLIEKLGLNAL
                     LMQIPIGLEDRLEGVVDLVTMKALYFDAGPNQDQLREGEIPSALLAEAQEKREELLDG
                     VSLSSDALMEAMLEDAVTEEILIKAIREATINLQMVPVFMGSAYRNKGIPPLLDAVVN
                     YLPNPSDVTNTALDLDNDEEEVVLVADDEMPTVALAFKLEDGQYGQLTYIRIYQGKIR
                     KGDELYNTRTRRKFRVGRLIRMHADTMEDLSEAGCGEIAALFGIDCASGDTFCDPKLN
                     YSMSSMYVPKPVISLAVHPVDKKASDNMAKALNRFTKEDPTFRTFVDPESNQTIIQGM
                     GELHLEVYVERMKREYKAVVEVGQPEVAYREAITQRADFVYTHKKQTGGSGQYAKVAG
                     YIEPLIATGVDEEDADYEFVDEIKGGTIPTEFIPSCDKGFQLAMKKGTQVGFPIIGVR
                     AVINDGAYHAVDSSDQAFQTAALGAFREAFENAKPVILEPIMKVEVVAPNEFQGSVFA
                     SVNQRRGVIISSNEDNQVSTILGEVPLSEMFGYSTILRSLTQGKGEFTMEFSKYGRVP
                     ASVAESLRKEYAEKRRQAQK"
     gene            3755..4462
                     /locus_tag="PLF67_07745"
     CDS             3755..4462
                     /locus_tag="PLF67_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89938.1"
                     /translation="MEIHELNELSPLRVFDESLGGGLGRGNLGVLVSRHGVGKTACLV
                     HLATDRLLHGEYVIHVSFSGNVEHVINWYKEVFREVAEGRSLDDAATVYNNILSNRVV
                     MNFSQENVTIDKVLSSLETLIKHGSFKADAILFDGYKLTVATEEDVRKIKEFAQKMNL
                     EVWFSVSPVRSDAVYDKYGVPDSMAKYVDLIDVLIGLVYSEELDKVVMTAVKAPGDIM
                     DKPMGVSLDPKTMLISK"
     gene            complement(4514..5542)
                     /locus_tag="PLF67_07750"
     CDS             complement(4514..5542)
                     /locus_tag="PLF67_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOE89939.1"
                     /translation="MAKAKKKRVRKKHRLKRILLLFVGALILWALVLLFAPADDPSLI
                     GAAIPDLELPSYEEGALVVSHPGYTLRYEEEHEQASWVAYRLTRADLYGEAERKDNFR
                     ADPSIPTGSATPDDYRGSGYDRGHLIPAADLVRSEEAMSGSFYMSNMSPQEPSFNRGI
                     WATLEAVVRNIANVDRAVYVVTGPVLTDGPYKTIGRNEVSVPNRYYKVILDYEEPTLK
                     AIGFVLPNKDSKRDVSEFALTVREVEELTGLDFFHRLPDHIEEELETSLDLTLWDLTP
                     FQVTAKARAEFLESGDALPPSEKPGVHAVVLDTLNRVLYIIRRESIALLEDAIGKRTL
                     KQAAPFLY"
     gene            complement(5594..6640)
                     /locus_tag="PLF67_07755"
     CDS             complement(5594..6640)
                     /locus_tag="PLF67_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HOE89940.1"
                     /translation="MDGKKNHEISIRSSAAEYLTYIAAIGDGEHALEIRYEDENIWLT
                     QKMMATLYNVSIPAINQHLKKIFDDNELDEHSVIKKYLITADDGKKYNTNHYNLQAII
                     AVGFKVNNERAVQFRKWANQIVKDYTIQGWVMDDERLKNFGTILTKDYFEKQLEKIRE
                     IRLSERRFYQKITDIYATAIDYDPSARATKRFFSAVQNKLHYAIHGQTAAEVIVERAD
                     AAKEHMGLTSWEGAPNGKIHTYDVVVAKNYLSDHELGQMQRIVSAYLDMAEVQAARHI
                     PMTMEDWEQRLNGFLTLWDREILQDAGRVSAALAKRHAETEFEKHRIVQDRTYRSDFD
                     RFSALEDLKPALDE"
     gene            6752..7114
                     /locus_tag="PLF67_07760"
     CDS             6752..7114
                     /locus_tag="PLF67_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HOE89941.1"
                     /translation="MDPLLQLGIIFAFTFAGDLLSKGLQLPIPGSIIGMVLTLLALLF
                     GVVKERHLDKTGDFLLSHMTFFFIAPAVGLLGYADLVSSIWLRLILVNIISFFLCYLS
                     ASWTVVGVNALIGRLRRG"
     gene            7107..7775
                     /locus_tag="PLF67_07765"
     CDS             7107..7775
                     /locus_tag="PLF67_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HOE89942.1"
                     /translation="MADPLFGITLSIVAFGIGIRIQKKTRSALANPLIIAMVLVMLVL
                     VVFDIPLEAYEAGGSFITFFLPPVTTVLALSIYRQRQVLQAYLIPIGAGTLTGSIVAI
                     ASTIFLSRAFGLEEIVVRSLASKSVTAAVAVALTDRLGGIPALTIASTVITGMTGNIL
                     APYLGKIFGVTDKVAHGVGIGTSSHALGTSRALELGEIEGSMSSISISFSALWTTLIL
                     AFVF"
     gene            7850..9424
                     /locus_tag="PLF67_07770"
     CDS             7850..9424
                     /locus_tag="PLF67_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE89943.1"
                     /translation="MAIPINMEELLHQRVIESARIEYKTDWNPAPILHSITAFANDFD
                     NLGGGYIMLDAGHPGFPLKGLDEDALDFIQKDLLNKCNLIEPRYIPVMEPVLIDGRHI
                     LVLWVPGGDDRPYKCPVNVYTEKGAKRSEKAYYIRKGSATIRANALEERELIGLARNI
                     PFDDRINNHAKIADMRSSLLSEYLHNVGSGLYEGSLSRTVEDVATDMRLVGGPSEMRK
                     PINVGLMFFNERPDHFFPYSRIEVVDKPDPTGIGMKERVFTGPLDRQLRDALSYIGNY
                     VIAEYVTKLPDRAEAVRVFNWPYRAVEEALSNAIYHRSYQIHEPITVTVTPDRMEILS
                     LPGPDRSITDDDLEKRVLVSSRYRNRRIGDFLKELKMVEGRNTGIPLIVKEMKQNGSE
                     PPIFKTDEDRSYFRVIFPIHRAFLRQSNHDVEEPKAEKSSKRRNREELRILVLEALEK
                     TGGLSTSELARELGYKSLHDTLRAVVKELAASKEVAYLHPDKPNSRNQKIFITKELSV
                     SDHSHGTDERVLFTTC"
BASE COUNT         2084 a         2465 c         2703 g         2210 t
ORIGIN      
        1 cctaactcct ttacttgcag attgctaaga agtaccatac acatcaggaa caccttgtac
       61 aaggagaacg tgatgaagaa acgtcattgg gccctcgggg cgatcgtcgg agtggtattg
      121 ttggccttgg gcatcttcct gatcttccag gaggagtcgt tcaagaaaat ctttgtctcc
      181 ttgctcgggg tatatatgat cggttcgggg ttttccaccc tgctgggact caaatcctat
      241 cagctcggtc cccggctcag gcctgctacc ctcatcaagg cgctgctcac ccttctgttg
      301 ggggtgatcg ccctgatcct gccgattctc gtcgccgacc tcagcttcct gattcttctg
      361 tacatcatcg ccacggagct tctcttcagc ggaatcattt cgatcatcaa ccttgtagcg
      421 gtccgaaatc tcgacatcgc tttttctccc ataatcggtg atgccctgat cagcctgatt
      481 ctctcgatgc tgctcttctt ctttcccaga caaatcggaa cggtgctgtt gaaaggggtg
      541 gggattctgg tgatcgcgat cggacttttc ttcatcatcg cttcgctcat cacccgacgg
      601 acggggaaac gggaagaagg aaaaacgatt gagggagaag ccgagattct cgagccttag
      661 aaggaaaaga tcgcttccgc tttttttcgg gcctcatcac tcgctttctt cgtcgggtgg
      721 ttggaaccta ggatgaagga atcgcagtag ttcgccttgt tcttgtcgct gatgaactct
      781 tcgacgtgct cggcgcagtc gtggtaggat ccgggggagt agaagcggca gttgacgcag
      841 ctgtgcaggg gctccgagca ggtcggacac agggtagcgt atccgatcga aagcgtggaa
      901 tcgatcgtcg tcgaacagtg ggaacagctg cgcatcactc gaactccaat cgcttgccca
      961 atgcagcgat ctccccctcg tcatagtgcg ccttcggaaa ggtcaggcgt ttgccgtcat
     1021 cggcgtaacg ggggatgatg tgaagatgca ggtggggaat ctcctgtccg ctcgcctcgc
     1081 cgttgttgat caccacgttg gtggcggcgc agcccagcac ttcccgaagc ttggcgtcgg
     1141 ctttttttgc gatcacgagc aggtgggaca acacctcgtc gggggtatcg gccaaggtgg
     1201 gatggacttc gttggtgatg atgagcaggt gccccttgtt caccgggttg atgtcgagaa
     1261 tcacgaagca gatctcgtcc tcatacacct ttgcggacgg aatggctccg gctcggattt
     1321 ttgtaaaaac agtctccatg gcacacctca aaccaagtat agcacagcta tggcggggat
     1381 tgaacaaaaa agagagatgg tgtagtatct gctggtgttt tggacccatc tccttatagg
     1441 gttgaactct acgacatcgg cctccttcgg ccacggacac atagtgttga acaatcattc
     1501 gaagaacata gaggtaatgg aagcatgact gacttgcgac acttgaggaa tattgggatc
     1561 agcgcccata tcgactcggg gaagacgacc ctttccgagc gaatcctcta ctatacaaat
     1621 aaaatccacg aagtccatga agtccgcggc aaggatggcg tcggcgccac gatggacagc
     1681 atggagctgg agcgggagcg gggcatcaca atcgcaagcg ccgcgacgaa cgtcgtgtgg
     1741 aagggaaatg agatcaacgt gatcgatact cccggccacg tcgactttac gatcgaggtc
     1801 gagcgctccc tgcgggttct cgacggcgcg attctcgttc tgtgctccgt cggaggagtg
     1861 cagagccagt cgatcaccgt cgaccggcag atgaagcggt acaaagtccc ccggatcgcc
     1921 ttcgtgaaca agtgcgaccg caccggggcc aacccgtaca aagtgaaaaa ccagctcatc
     1981 gagaagctgg ggctgaacgc cctcctgatg cagattccga tcggtctgga ggatcgcctg
     2041 gaaggggtgg tggaccttgt cacgatgaag gcgctctact ttgacgccgg tccgaatcag
     2101 gatcagcttc gggaagggga gattccttca gcgcttctgg cggaagcgca ggagaagcgc
     2161 gaggagctgc ttgacggagt atccctttcc agcgatgccc tgatggaagc aatgctcgaa
     2221 gatgcggtta ccgaagagat cctgattaaa gcgattcggg aagcgacgat caatcttcag
     2281 atggtccccg tgtttatggg ttccgcatac cggaacaagg gaatcccccc gcttctggat
     2341 gccgtggtga actatctgcc caacccctcg gatgtcacca acacagcctt ggatctggac
     2401 aacgatgaag aagaagtcgt ccttgtggcc gatgacgaga tgccgacggt ggcgctcgca
     2461 ttcaagcttg aagacggtca atatgggcag ttgacgtata tccgcatcta tcagggaaag
     2521 atccgcaagg gggacgagct gtacaacacc aggacgcggc ggaaattccg agtggggcgg
     2581 ttgatccgga tgcatgccga tacgatggag gacttaagcg aagccggctg cggcgaaatc
     2641 gctgcgctct tcggcatcga ctgcgccagc ggcgatacgt tctgcgatcc gaagctcaac
     2701 tattcgatga gctcgatgta cgtgccgaag ccggtcatct ccctggcggt tcaccccgtc
     2761 gacaagaaag cctccgacaa catggcgaaa gctttgaacc ggttcaccaa ggaggatccg
     2821 accttccgca ccttcgtcga tcccgaatcc aaccagacga tcattcaggg aatgggtgaa
     2881 ctgcaccttg aagtatatgt ggagcggatg aagcgggaat acaaagcggt ggtcgaagta
     2941 gggcagccgg aggttgcata cagggaagcg atcacccagc gggccgactt cgtctacacc
     3001 cacaagaagc agaccggcgg ttccggtcaa tatgcgaaag tcgccgggta tatcgagccg
     3061 ctcatcgcta caggcgttga tgaagaagat gccgattacg agttcgtcga tgagatcaag
     3121 gggggaacga ttcccaccga gttcatccca tcctgtgata aaggcttcca gctggcgatg
     3181 aagaagggga cccaggtcgg tttcccgatc atcggagtcc gggcggtgat caacgacgga
     3241 gcgtaccatg cggtggactc ctccgaccag gcgttccaga ccgccgcttt gggggcgttc
     3301 cgcgaggcat tcgagaatgc gaagccggtg attctggagc cgatcatgaa agtggaagtg
     3361 gttgccccca acgagttcca gggaagcgtg ttcgcttcgg taaaccagag gcggggagtg
     3421 atcatctctt ccaacgagga caaccaggtc tcgacgatcc ttggggaggt tcccctctcg
     3481 gagatgttcg gatattcgac gatccttcgg tcgctgaccc agggaaaagg tgaattcacg
     3541 atggagttca gcaagtatgg ccgggtgccg gcctcggttg ccgagagctt gcgcaaggag
     3601 tatgcggaga agcgccgcca agcgcagaaa tgatgattga caggaatccc cggaagagcc
     3661 gttccggggt tcttcttttt gtaaagaagt atcaaagtgg gatatttcgt tgtatactga
     3721 aggcagaact actttgggtt aaggaggacg ttatatggaa atacatgagt tgaacgaatt
     3781 aagccccctg cgcgtttttg atgaatctct cggcggcgga ctgggacggg gaaacctcgg
     3841 ggttctcgta tcacgccatg gggtcggaaa gaccgcatgt ctggtgcact tggctaccga
     3901 tcgcctgctt catggcgagt atgtaatcca cgtctccttc tcgggaaacg tcgaacatgt
     3961 gatcaactgg tacaaggaag tgttccggga ggtggctgaa gggcggagcc tcgatgacgc
     4021 tgcgacggtg tacaacaata ttctttccaa ccgggtcgtc atgaacttca gtcaggaaaa
     4081 cgtgacgatc gataaagtcc tttccagttt ggagaccctg atcaagcatg gatcgttcaa
     4141 ggcggatgcg atcctctttg acgggtacaa gctgaccgtt gcgacggaag aggatgttcg
     4201 aaagatcaag gagttcgccc agaagatgaa ccttgaggta tggttcagcg tctctccggt
     4261 tcgttccgat gcggtgtatg ataaatatgg cgttcccgat tcgatggcga agtacgtgga
     4321 tctgatcgat gtcctgatcg ggctcgtcta cagcgaagag ctggacaagg tcgtcatgac
     4381 cgcggtgaag gcccccggcg acatcatgga caagccgatg ggggtgagcc tcgatccgaa
     4441 gacgatgctg atttcaaagt aggaaacagg tcgcattctc cccaagacag ccggcgaaag
     4501 ccggctgtcg ccgtcaatag aggaagggtg ccgcctgttt cagggttctt ttcccgatcg
     4561 catcctccaa gagggcgatg ctttcccttc ggatgatgta gagaacccga ttcagcgtat
     4621 ccaaaacgac cgcatgcacc cccggtttct ccgatggcgg gagcgcatcg cccgattcga
     4681 ggaactccgc cctcgccttg gcggtcactt ggaagggggt gagatcccag agcgtcagat
     4741 cgagactcgt ctccagctcc tcttcgatgt gatcggggag gcggtggaag aaatccagcc
     4801 cggtcagttc ctcgacctcc cgaaccgtca gggcgaactc gctcacatcc cgttttgaat
     4861 ccttgttggg aagcacgaac ccgatcgctt tcagcgtcgg ctcctcgtag tcgaggatta
     4921 ctttgtagta ccggttgggg acggatactt cattcctgcc gatcgtcttg tacggcccgt
     4981 cggtgagcac cgggccggtg accacgtaga cggcgcggtc cacgttggcg atgttgcgga
     5041 ccaccgcctc gagcgtcgcc cagatgccgc ggttgaagga gggttcctga gggctcatgt
     5101 tggacatgta gaacgagccg ctcatcgcct cctcgctgcg gaccagatcc gcggcgggaa
     5161 tgaggtgtcc ccggtcgtag ccgcttccgc ggtaatcgtc cggcgtcgcc gatccggtgg
     5221 gaatcgaggg gtcggcccgg aagttgtcct tccgctccgc ctcgccgtag agatccgccc
     5281 gggtcagccg gtaggcgacc caggaagcct gttcatgttc ttcctcatag cgcagggtgt
     5341 acccggggtg ggagacgact agtgcacctt cttcatagga gggaagctcc agatcgggaa
     5401 tggctgctcc tatcagggac gggtcgtcgg cgggggcgaa gaggagaacg agagcccata
     5461 ggatgagcgc accgacgaag agcaggagaa tccgtttcag gcgatgtttc ttgcgaacgc
     5521 gtttcttttt tgctttagcc atggattcac ggtagccaat gagcaaacgg ttgtccagag
     5581 tccttccgtc gatttactcg tcgagggcgg gcttcaggtc ttcaagcgct gaaaatctgt
     5641 caaaatcgct tctgtacgtt cgatcctgaa cgattctatg cttttcaaat tccgtttcgg
     5701 catgtctttt cgcaagggcg gcggagaccc tgccggcgtc ctgcaggatc tccctgtccc
     5761 acaaggtgag aaaaccgttc agtctctgtt cccaatcttc catggtcatg ggaatatgcc
     5821 tggcggcttg gacttccgcc atgtcaagat acgcggagac gatccgctgc atctgtccaa
     5881 gctcatgatc cgacaaataa ttcttggcta cgacgacatc gtatgtgtgg attttcccgt
     5941 ttggcgcgcc ttcccaggaa gtaagcccca tatgctcctt ggccgcatcg gctcgctcta
     6001 caatgacttc ggctgcggtt tgaccgtgta tcgcataatg cagcttgttc tgaaccgctg
     6061 aaaagaatct ctttgtggcc cttgcactcg ggtcataatc gatggcggtg gcatagatgt
     6121 cggtgatctt ctgatagaat cgtctttcgg agagccggat ttcacggatt ttttccagtt
     6181 gtttttcaaa gtagtctttt gtgaggatgg tgccgaaatt cttgagtctc tcgtcatcca
     6241 tgacccagcc ctgaatcgtg taatccttga cgatctgatt cgcccacttc ctgaactgaa
     6301 ccgcccgctc gttgttgacc ttgaatccga cggcgatgat cgcctgcagg ttatagtggt
     6361 tggtattata ttttttcccg tcatcggcag ttattaagta tttcttaata actgaatgtt
     6421 catcaagttc attgtcatca aatatctttt ttaaatgctg gtttattgcc ggaatgctta
     6481 cgttgtacag cgttgccatc atcttttggg tgagccagat attttcatct tcgtatcgta
     6541 tctccagagc atgttcgccg tcgccgatcg ctgcgatata cgtcagatat tcagctgcag
     6601 atgagcgaat ggagatttca tggttcttct tgccatccat tcaacgagtt cctccttcga
     6661 ttccttaaaa ataccacaaa ccggatccgt gctcaatttc aagagtccga accccttcca
     6721 tcgggaaggc gctttacagt aaggttgccg tatggatccg ctccttcagc ttggaatcat
     6781 tttcgccttc accttcgccg gtgacttgtt gagcaagggc cttcagcttc cgatccccgg
     6841 ttcgatcatc gggatggtgc ttacgttgct ggccctcctc tttggtgtcg tcaaggaacg
     6901 tcatcttgac aagacggggg attttcttct ttcccacatg acctttttct tcatcgcccc
     6961 ggccgtggga ttgctcgggt atgccgatct ggtctcttcg atctggctcc ggctcattct
     7021 ggtgaatatc atctccttct tcctctgcta cctgagcgca agctggaccg tcgtcggggt
     7081 caacgctctc atcgggaggc tgcgccgtgg ctgatccgct ctttggcatc accctctcga
     7141 tcgtcgcctt cggcatcggt atccggatcc agaaaaagac aagaagcgcc ttggccaatc
     7201 ccctgatcat cgcgatggtt ctggtcatgc ttgtgctcgt cgtctttgac atcccgctgg
     7261 aagcgtatga ggccggcgga tccttcatca ccttcttcct tccgcccgtc accaccgttc
     7321 tggcgctttc gatctaccgc cagcgccagg tcctgcaagc gtatctcatc cccatcggcg
     7381 ccgggacgct gacaggctcg atcgtggcga tcgcctcgac gatcttctta agccgtgcct
     7441 tcggccttga agagatcgtg gtgcgctccc ttgcaagcaa atccgtcacc gctgcggtgg
     7501 cggtggcgct gaccgaccgg cttggaggga ttcccgcctt gacgatcgcc tcgaccgtga
     7561 tcaccggaat gacgggaaac atccttgccc cctatctggg gaagatcttc ggtgtcaccg
     7621 acaaggtcgc ccacggggtg gggatcggaa catccagcca cgccctcgga acgagcaggg
     7681 cgctcgagct gggcgagatc gagggttcga tgagcagcat ctcgatcagc ttttccgccc
     7741 tttggaccac gttgattctc gcgtttgtgt tctgacttgc cctttgactg tccctttgtt
     7801 acagtatggg agagttaatg ggagagtaag gttcaggtag gaggaaatga tggcgattcc
     7861 gatcaatatg gaagaactgc ttcatcagcg tgtgatagag agcgcccgca tagaatacaa
     7921 gacggactgg aatcctgcgc cgattctgca ttccataacc gcttttgcca atgattttga
     7981 caacttgggc ggcgggtaca tcatgttgga cgccgggcat cccggattcc ccctcaaggg
     8041 gcttgatgaa gatgctttgg acttcatcca gaaggatctc ctcaacaaat gcaacctcat
     8101 cgagccgcga tacataccgg tcatggaacc ggtgttgatc gacggacggc acattcttgt
     8161 tttgtgggtt cccgggggag atgacagacc atacaaatgc ccggtcaacg tatacacgga
     8221 gaaaggagcc aaaaggagtg aaaaagcata ttacatccgc aagggttctg ctaccatccg
     8281 ggcaaatgct ttggaagagc gcgagctgat cggactggca agaaacattc cttttgatga
     8341 cagaatcaac aatcatgcaa agatcgcgga catgcgttca agcctgcttt cagagtatct
     8401 tcataacgtt ggcagcggat tgtatgaagg ctcgctttcc cgcacggttg aggacgtggc
     8461 aacggacatg agacttgtcg gggggccttc ggaaatgaga aaacccatca atgtgggctt
     8521 gatgtttttc aacgaaagac cggatcattt ctttccgtat agccggattg aagtagtgga
     8581 caagcccgac ccgacaggga ttggaatgaa agaacgggtt tttacaggcc ccttggatcg
     8641 gcagctgcgg gatgcactgt cgtacatcgg aaattacgtc atcgccgaat atgtgacgaa
     8701 actgcccgat cgggcggagg cggttcgggt gttcaattgg ccgtatagag cggtggagga
     8761 agctctgagc aatgccatct accatcgctc atatcagatc catgagccga tcaccgtaac
     8821 ggttactccc gatcggatgg aaatcctgag tcttccgggt cccgaccgat cgatcacgga
     8881 tgatgatctg gaaaagaggg tgcttgtctc cagccgatat cgaaatcggc gcatcgggga
     8941 tttcctgaaa gaattgaaga tggtcgaggg aagaaatacc ggtattccgc tgatcgtgaa
     9001 agagatgaaa cagaacggtt ccgaaccgcc gatcttcaag acggacgagg acagaagcta
     9061 ttttcgagtg atattcccga ttcatcgggc gtttctcagg caaagcaacc atgatgtgga
     9121 agaaccgaag gcggagaaaa gctccaagcg gaggaacagg gaagaactca ggatacttgt
     9181 gcttgaggcg ttggaaaaaa cgggtggtct ttcaacaagc gagctggctc gggaacttgg
     9241 ctataagagt ttgcatgaca cgcttcgagc cgttgtcaaa gagctggcgg catccaaaga
     9301 ggttgcatac ctccatccgg acaagccgaa cagcagaaac cagaagattt ttataacgaa
     9361 agaactatcg gtttcagatc atagccatgg aacagatgaa agggtgcttt ttaccacctg
     9421 ttgacagtat tccacgcttc tgattctcgc gtttgtgttc cg
//
LOCUS       DAOSLI010000059         9344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_7044_length_9344_cov_29.738831, whole genome shotgun sequence.
ACCESSION   DAOSLI010000059 DAOSLI010000000
VERSION     DAOSLI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7044_length_9344_cov_29.738831"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="PLF67_07775"
     CDS             complement(<1..1250)
                     /locus_tag="PLF67_07775"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOE89944.1"
                     /translation="MSKVVSIVGAQWGDEGKGRMIDYLAQSSQVVIRFQGGDNAGHTV
                     INDKGKFALHIIPSGIFNPDTMNIVGAGTVVNFDTMAEELASITKAGVEVDNLFIDVR
                     AHLIMPYHRQLDGAQERSKSEKAQIGTTGRGIGPCYSDKATRSGLRAGDLHDLERART
                     RLEMLLPQKNRELAFYGLPEADIEELMTLLERWAATYRDKIIDTLPVVRDAYETGQKI
                     LLEGQLGVMRDLDWGIYPYTTSSSPTSGGAAVGSGLGPRRIDEIIGVTKVYSTSVGGG
                     PFMTELLDEVGEKLRSIGKEFGATTGRPRRCGWFDAVATEFSCWINDFTALALTKLDV
                     LDTFETIKICTGYMIGGEYYNHLPETALQELAKPVYEEHKGWMSDTTGARSWDDLPKE
                     AQAYCRRIEELVGCPIKYISVGPER"
     gene            complement(1247..1765)
                     /locus_tag="PLF67_07780"
     CDS             complement(1247..1765)
                     /locus_tag="PLF67_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOE89945.1"
                     /translation="MSDNALYVSYNTVHKLVKSLSERLLDSGYDPEVIVAIGSGGFIP
                     ARIMKTFINRPIYAVGISYYGVDNKHRDHPTKIQWIDEVKSQLRGKKVLLIDEVDDSR
                     VTLAYCIGELLKYEPEEIAVLVLHNKRKEKDVEFPSEIKRYFSGMETDDVWIKYPWDA
                     VEIEEHTAKEEA"
     gene            complement(1768..2328)
                     /gene="hpt"
                     /locus_tag="PLF67_07785"
     CDS             complement(1768..2328)
                     /gene="hpt"
                     /locus_tag="PLF67_07785"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOE89946.1"
                     /translation="MNNSLSIPPLAKDLVRVLIDAATIERRVDELARQITEDYQNLEG
                     DLILVGVLKGSFIFIADLARKMMKKHVIDFIALSSYDGGTSGNVRLLMDTRENMENKH
                     VLIVEDILDSGNTLDYLTRSFMTRSPRSVKTAVLLDKPERHEVDIKVDYIGFTIPDVW
                     VVGYGLDYNERYRTLPYIAEMKPQEG"
     gene            2499..4484
                     /locus_tag="PLF67_07790"
     CDS             2499..4484
                     /locus_tag="PLF67_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HOE89947.1"
                     /translation="MRLTKNQAHKALSIMVRSRRFEELVAQFFHHKEMHGTTHLSIGQ
                     EAAQAGLSLALKEGDWIVPTHRCHGHTIARGSGEEVMFSEMFGSRHGIVKGLGGSMHM
                     TDVQTWNTGSSSVVGSGVNLATGLGFALKQQGSDSLSVAIFGDGASSRGAVHESMNLA
                     SVLNLPVLFFCENNGYGMSASRDRMVSAPSIAKRAEGYNMPHNTVDGNDVEAVYAAAR
                     EAVRSIRSTGRPYLLEVLTYRLCGHSKSDDCHYRTKEEEAEWEAKDPITRFCRTLIED
                     ELFSEDEVSEIILSGRKQVEEAAMRARAVKDEHLTFEEAEALVYAPEDPQVYGVVNQT
                     SRLTYREAIRFALAEEMARDKAVHLIGEDIGLYGGCFKVTGDLHANFQDRVHETPVSE
                     EAITGMGVGASFLGIRPVVEIMYGDFATLASDPIINHAAKAHFISAGQLSAPVVVRMP
                     VGSGTGHGAQHTQSLESMFTNVPGLIVVAPSCPGDAKALLKSAIRSNNPVLFFEHKGL
                     YDTIGPVGDKEYLMPLGKAIVKKRGSDVTIVAYSKAVTTALSAASILSAQDEIEVEII
                     DLATLKPLDTETILRSVKKTGRLLVVHDSPEFAGFGAEILAAVAGDEPTLRSLKTAPK
                     RLCGKELPIPFAQELEKGATVSKEEIVKSVRLLFTAV"
     gene            4558..6096
                     /locus_tag="PLF67_07795"
     CDS             4558..6096
                     /locus_tag="PLF67_07795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOE89948.1"
                     /translation="MDIDVKNLHPLEVRLLRHVSLGEAIDAKRLIGELGYNVGQCNQS
                     FSWLEAKGFIKEEARIRRTLYELTDVGRFQAQEGTPAERIFHFLKEHGPAPLPKIAES
                     LKLEQSEVGSSFGLLSKGGFVGMNEANNAEAKAQDLEGMILLTRTVLKKGLDGLLDDD
                     TLTSEERTAVSRIAKKRGAATSPFKVVEREEVTYTLTEAGRKAKEAIIALKITGEELG
                     LVTSEILSTGSWKNSTFRPYALNAPTSRLIVGRHNAYADYLQWVKNKLISLGFEEFDG
                     SLVESEFWNGDALFMPQFHSARDIHDVYYVKEPRHAKTIEEPWLSQVAATHENGWKTN
                     SRGWGYTFDREFTRRQILRSQGTVLSARQLPKAKVPGKYFGVVRCFRYDQVDATHGAD
                     FYQTEGIVLGEEVNLKNLLGLLKMFAEEIAGAEEVRYVPGYFPFTEPSIEVHIKHPTL
                     GWFELGGSGIFRPEVTGPLGIDVPVLAWGLGIDRMALMHLGLNDLRELFTPNIESVRL
                     RRGK"
     gene            6096..7793
                     /gene="pheT"
                     /locus_tag="PLF67_07800"
     CDS             6096..7793
                     /gene="pheT"
                     /locus_tag="PLF67_07800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOE89949.1"
                     /translation="MPKIETNARYFYELVGKTLTTEELEAILPVAKAELDDYDGEILK
                     IELNDTNRPDLWSPAGIARLLRSYWEIEAPLYDFFSTSEETFDHEGRVVIVDASVTPI
                     RPYGIGFAARGHKLSGADLEALIQSQEKICWNFGQKRRSIAMGIYRSALITYPVHYRG
                     ADPDRTRFVPLGMEEELSLREISTVHPKGKEYGHIHAHDPLFPYLHDDKDQTLSFPPV
                     INSASIGAVEEGDEELFVEFSGTELKDLLLAAAIVACDMADMGYEILPVKVVFPEETE
                     FGKEITVPYYFQEPASCTVEEIHKTLGDPLTDDEIVRGLKRMGIYAVVDEGRVYATVP
                     EWRNDFLHAVDLVEDVMIGHGLERFAPVMPTDFTVGRLTEAEELGRKIKDLMVGLGFQ
                     EMMYNYLGSRREYIDNMRYPAEKCIIIANPMSENYEVVRPSILPSLLESESVSAHAPF
                     PHRIFEVGKVAYIDDSTNSGTITRNKLGFLASDSVMGYNEVSSYVSTLFYFLSTEYTL
                     AEAGEDGRFIPGRSARIMVDGKEVGIFGEIHPQVLENWKSDTPTVGCEIDLDTLLGH"
     gene            complement(7879..8823)
                     /locus_tag="PLF67_07805"
     CDS             complement(7879..8823)
                     /locus_tag="PLF67_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89950.1"
                     /translation="MRILQRSILFAFVLTIGTFGLFAAPLSEQPVEQVQETPAVIVES
                     SRRSPVTLTLDFTAEGETYRYSYTQQIIQAPGTPVRLPSRSQIRFNPGQIGFLASWSD
                     GKNTYEAGETITMPDKDTSLTATYEEGVLFYDPIHEIEYYSSDRVVTVPTIPDDDEDR
                     VFVGWYDRQSDTLLTEDTYERTTRGACFRPVYKSLVFTRIALHSYNSNAVPRNTALSL
                     SFAYRNRGDFVMNDITASLECDHGLVRILDPQPQRRTLRPNESNHHPDFFGRRTSTDS
                     FTFVIDGSVPSGSELPFTLTVTDEDGVSFSKTFTLIVQ"
BASE COUNT         1939 a         2674 c         2783 g         1948 t
ORIGIN      
        1 cgctccggac cgacggagat gtacttgatc ggacagccta ccagttcttc gatccgcctg
       61 cagtacgcct gagcctcctt gggcaggtca tcccaggagc gggcgcccgt cgtgtcgctc
      121 atccacccct tgtgctcttc atagaccggt ttggccagct cctgaagggc ggtttccgga
      181 aggtggttgt agtattcccc gccgatcatg tacccggtgc agatcttgat cgtctcgaag
      241 gtgtccagca cgtccagctt ggtcagggcg agggcggtga agtcgttgat ccagcaggag
      301 aactccgtgg ccaccgcatc gaaccagccg caccggcgag gccggccggt cgtcgctccg
      361 aactcctttc cgattgagcg gagcttctct ccgacttcgt caagcagctc ggtcatgaac
      421 ggaccgccgc cgacgctggt cgagtagacc ttggtgactc cgatgatctc gtcgatcctt
      481 cgcggaccca ggccgcttcc gaccgccgct ccgccgctgg tgggggagga gctggtcgtg
      541 tagggataaa ttccccagtc gagatccctc atcaccccga gctgcccttc aagcaggatc
      601 ttctgcccgg tctcatacgc atcccggacg accggaaggg tgtcgatgat cttgtcccgg
      661 taggttgccg cccatcgttc cagcaaggtc atcaattcct cgatgtccgc ttccggcagg
      721 ccgtagaagg caagctcccg gttcttctgg ggcagcagca tctcaaggcg ggttcgggcg
      781 cgctcaaggt catgaagatc gccggccctc aggccgcttc gcgtcgcctt gtcgctgtag
      841 caggggccga tcccccggcc ggtggtgccg atctgggctt tctcgctctt cgagcgctcc
      901 tgggcgccgt cgagctggcg gtggtagggc atgatcaggt gggctcggac atcgatgaag
      961 aggttgtcca cctcgactcc cgctttggtg atcgaagcaa gctcttcagc catcgtgtcg
     1021 aagttcacca ccgttcccgc cccgacgatg ttcatcgtat cgggattgaa gattccggaa
     1081 ggaatgatgt ggagggcgaa cttgcccttg tcgttgatga cggtgtgacc ggcgttgtcc
     1141 ccgccctgga agcggatcac cacctgcgaa gactgggcga ggtagtcgat cattcgaccc
     1201 tttccctcat cgccccattg ggctccaacg attgatacaa ccttgctcat gcttcctcct
     1261 tggcggtgtg ctcttcgatc tccaccgcat cccacggata cttgatccag acatcatcgg
     1321 tttccattcc gctgaaataa cgtttgattt cggaggggaa ctccacgtcc ttctccttgc
     1381 gcttgttatg caataccaat acggcgatct cctcgggctc atactttaac agttcgccga
     1441 tgcagtacgc caaggtgacc cggctgtcat cgacttcgtc gatcagcagg acttttttcc
     1501 cccgaagctg gcttttcacc tcgtcgatcc attggatctt cgtcggatgg tcgcggtgct
     1561 tgttgtcgac tccgtagtag gagatgccca ccgcatagat cgggcggttg atgaaggtct
     1621 tcatgatgcg ggcggggatg aagccgccgc tgccgatcgc gacgatcacc tcggggtcat
     1681 accccgaatc gagcagccgc tcactgagcg atttgacgag cttgtgaacg gtgttgtagg
     1741 aaacatacag agcgttgtca ctcatgctta cccctcctga ggtttcatct cggcgatgta
     1801 gggaagggtt cggtagcgtt cgttgtaatc gaggccgtag ccgacgaccc agacgtcggg
     1861 tatcgtgaag ccgatgtaat cgaccttgat atcgacctcg tgccgctccg gtttgtcaag
     1921 caggacggcg gtctttaccg aacgcgggct tcgggtcatg aagctccggg tcaggtaatc
     1981 gagggtgttc ccgctgtcga ggatgtcttc gacgatcaaa acatgcttgt tctccatgtt
     2041 ttcacgggtg tccatcagaa ggcggacgtt accgctggtt cccccgtcgt aggaggaaag
     2101 ggcgatgaaa tcgatgacgt gttttttcat catcttgcgc gccagatcgg cgatgaagat
     2161 gaaggagcct ttgaggactc cgacaagaat cagatctccc tccaggtttt ggtaatcttc
     2221 ggtgatctgg cgggccagct catcgactcg acgttcgatc gtggcggcgt cgatcaggac
     2281 gcggaccaag tctttggcta gtggcggaat ggacaagctg ttgttcatag gaaccccctt
     2341 gggtacgatg taaggattat ataaaaagag gctgttgtca acgagagctc ataaaacgat
     2401 tcctcaacat tgacagggga aggattggcg cgtacgatac aaaagaccac caactcatgt
     2461 gggaaaggcg ggactgcata tcctgccggg gagcacagat gagactgaca aagaaccagg
     2521 cgcacaaagc gctttcgatc atggttcgtt cgcgccgatt cgaggaattg gtcgctcaat
     2581 tttttcacca taaagagatg catgggacca cccatctttc aatcgggcag gaggccgctc
     2641 aagccggtct ctccctggcg ttgaaagagg gggattggat cgtccccacc catcgctgcc
     2701 acggtcatac gatcgcccgg ggcagcggcg aagaggtgat gttcagcgag atgttcggct
     2761 cgcgccatgg aatcgtgaag ggcctcggcg gctcgatgca catgacggat gtccagacgt
     2821 ggaacaccgg ctcctccagc gtggtcggaa gcggcgtgaa ccttgcgacc ggcttgggct
     2881 tcgccctcaa gcagcaggga tccgattcgc tctcggtggc gatctttggc gacggggcga
     2941 gcagccgggg ggcggtgcac gaatcgatga accttgcttc cgttttgaat ctgccggtgc
     3001 tttttttctg cgagaacaac ggctacggga tgagcgcttc ccgtgatcgg atggtcagcg
     3061 ccccctcgat cgccaagcgg gcggaaggat acaacatgcc ccataacacc gtcgacggca
     3121 atgatgtcga agcggtgtac gcggcagctc gggaggcggt ccgatctatc cgttccaccg
     3181 gcaggccgta tcttctggaa gtgcttacct accgcctgtg cggacactcc aaaagcgatg
     3241 actgtcacta tcgaacaaaa gaagaggaag cggaatggga ggcgaaagac cccatcaccc
     3301 gtttttgccg gaccctcatc gaagatgaac ttttcagcga agacgaggtt tccgaaatca
     3361 ttctctccgg ccgcaaacag gtcgaagaag ccgccatgag ggctcgagcc gtcaaggacg
     3421 aacacctcac ctttgaagaa gccgaggcgt tggtctacgc tccggaagat cctcaagtct
     3481 atggcgtcgt caatcagacg agccgtttga cctatcggga agcgatccgc tttgcattgg
     3541 ccgaggagat ggcccgtgac aaggcggtgc acctcatcgg ggaggatatc ggcctctacg
     3601 gcggatgttt caaagtgacc ggcgatctgc atgcgaactt tcaggaccgg gtccatgaaa
     3661 cccccgtcag cgaagaagcg atcaccggga tgggggtcgg cgcctcgttt ctgggcatcc
     3721 gaccggtcgt cgagatcatg tacggagact tcgccaccct tgccagcgac ccgatcatca
     3781 accacgcggc gaaagcccat ttcatcagcg ccggccagct ctccgccccg gtcgtcgtcc
     3841 ggatgcccgt cggcagcggc accggccacg gagctcagca cacccaatcc ctcgaatcga
     3901 tgttcaccaa cgttccgggc ctgatcgtcg tcgctccgtc gtgcccgggg gatgcaaagg
     3961 cgctcttaaa gagcgcgatc cgctccaaca accccgtgct gtttttcgaa cacaagggct
     4021 tgtacgacac catcggaccg gtcggggaca aggagtatct catgccgctg ggcaaagcga
     4081 tcgtgaagaa gcggggaagc gacgtgacga tcgttgcata ctccaaagcg gtgacgacgg
     4141 ccttgtccgc cgcttcgatc ctttccgctc aggacgagat cgaggtcgag atcatcgacc
     4201 ttgccacgct caagcccctc gatacggaga cgatcctcag gtcggtcaaa aagaccggcc
     4261 gattgctcgt ggttcatgat tcgcccgagt tcgccggctt cggtgcggag atcctcgctg
     4321 cggtcgccgg cgacgagccg actcttcgtt ccttgaagac cgctccgaag cgcctctgcg
     4381 gcaaagaact gccgattccc ttcgcccagg aactggagaa gggggcgacc gtatcgaagg
     4441 aggagatcgt aaagagcgtc cgcttgctct tcaccgccgt atagcctttc ctttttcccg
     4501 ctcaatcgat agaatacaaa ccacttgggc catccggtcc gaagaggagt attgatgatg
     4561 gatatcgatg tgaagaacct ccatcccttg gaggtccgtc tcctgaggca tgtgtccttg
     4621 ggagaggcga tcgacgccaa acgcctgatc ggcgagctcg ggtacaatgt cggccaatgc
     4681 aaccagagct tcagctggct tgaagcgaaa ggctttataa aagaagaagc gcggatacgg
     4741 cggaccttgt atgaactgac cgatgtgggc aggttccaag cccaagaggg caccccggcc
     4801 gagcggatct ttcatttcct caaggagcat ggccccgctc cgctgccgaa gatcgccgag
     4861 agcctgaagt tggagcagag cgaagtcggc tcgtccttcg gcctgctttc caaaggcggg
     4921 ttcgtcggca tgaatgaagc aaacaacgcc gaagccaaag cccaagattt ggaaggaatg
     4981 attcttttga cccgaacggt gctgaagaag ggcctcgatg ggctcctgga tgacgatacc
     5041 ctgacaagtg aagaaagaac ggcggtctcc cggatcgcca agaagcgcgg ggcggcgacc
     5101 tcgccgttca aggtggttga gcgggaggag gtgacctaca ccctcaccga agcgggccgg
     5161 aaagccaagg aggcgatcat cgccctcaag atcaccggcg aggagctggg tttggtaacg
     5221 tccgagatcc tttccaccgg atcgtggaaa aactcgacgt tccgtcccta tgccttgaac
     5281 gctcccacca gccgcctcat cgtcggccgg cacaatgcgt acgccgacta cctgcagtgg
     5341 gtgaagaaca agctgatcag cctcggcttc gaggagttcg acggctcgct ggtggaaagc
     5401 gagttctgga acggggacgc cctcttcatg ccgcagttcc actccgcccg ggatatccat
     5461 gacgtctact atgtcaaaga accccgacac gccaagacga tcgaggagcc gtggctcagc
     5521 caggtcgcag cgacccacga gaacggatgg aagacgaaca gccggggttg gggctacacc
     5581 ttcgaccgcg agttcacccg ccgccagatc cttcgcagcc aggggacggt cctttccgcc
     5641 cgccagcttc ccaaagcgaa ggttcccggc aaatacttcg gcgtcgtccg gtgcttccgg
     5701 tacgaccagg tcgatgcgac ccacggagcg gatttctatc agaccgaggg aatcgtcctc
     5761 ggcgaagagg tgaacctgaa gaacctgctc ggtcttttga agatgttcgc cgaggagatc
     5821 gccggagccg aggaggtccg ctacgtgccc ggctatttcc ccttcaccga accctcgatc
     5881 gaagtgcaca tcaagcaccc caccttgggc tggttcgagc tcggcggctc gggaatcttc
     5941 cgccccgagg tgaccgggcc cttgggaatc gatgttcccg ttcttgcctg ggggcttgga
     6001 atcgaccgga tggcgctgat gcacctcggc ctgaacgacc ttcgggaact ctttaccccg
     6061 aacatcgaat cggtccgctt aaggagggga aagtaatgcc aaagattgaa accaatgccc
     6121 gctacttcta cgagctggtg ggaaaaacgt tgaccaccga agagcttgaa gcgatccttc
     6181 ccgtcgcaaa agccgagttg gatgactatg acggggagat cctgaagatc gagctcaacg
     6241 atacgaaccg ccccgacctc tggtcgccgg ccgggatcgc ccgcctgttg cgatcgtatt
     6301 gggagatcga agctcccttg tatgatttct tctccacgtc ggaggagacg ttcgaccatg
     6361 aggggagggt ggtgatcgtc gatgcgtcgg tcaccccgat ccgcccctat ggaatcgggt
     6421 tcgccgcccg cggccacaag ctctcgggcg ccgatctgga agccttgatc caaagccagg
     6481 agaagatctg ctggaacttc gggcagaagc gcaggtcgat cgcgatgggg atctaccgtt
     6541 cggcgttgat cacctatccg gtccactacc ggggtgccga tcccgacagg acgaggttcg
     6601 tcccgcttgg gatggaggag gagctctctt tgagggagat cagcacggtc cacccaaaag
     6661 ggaaggagta cgggcacatc cacgcacatg atccgctttt cccatacctg cacgatgaca
     6721 aggaccagac cctctccttc ccgccggtga tcaacagcgc ctcgatcggg gcggttgaag
     6781 agggggacga ggagctcttc gtcgagttca gcggaaccga gctgaaagac ctcctgcttg
     6841 cagctgcgat cgtcgcctgc gacatggcgg acatggggta tgagattctt cccgtcaaag
     6901 tcgttttccc cgaagagacg gagttcggca aggagatcac cgtaccgtac tatttccaag
     6961 agccggcctc ctgcaccgtc gaggagatcc acaagacgct cggcgatccg ctcaccgacg
     7021 atgagatcgt ccgagggttg aagcgaatgg ggatttacgc ggtggtcgac gagggcaggg
     7081 tctatgcgac cgtgccggag tggcgcaacg actttcttca cgccgtagat ttggttgaag
     7141 acgtgatgat cggccacgga cttgagcgct tcgctccggt gatgcccacc gacttcaccg
     7201 tcggccgcct tacggaagcg gaggagctcg ggcggaagat caaggatctg atggtcggtc
     7261 tcggtttcca ggagatgatg tacaactacc tcggctcgag gcgcgagtac atcgacaaca
     7321 tgcggtatcc cgctgaaaag tgcatcatca tcgccaaccc gatgagcgag aactacgagg
     7381 tggtccgccc ctcgattctt cccagccttc tggagagtga aagcgtcagc gcccatgctc
     7441 cgttccccca ccggatcttc gaggtcggca aggtcgccta catcgatgat tcgacgaaca
     7501 gcgggacgat cacccgaaac aagctcggct ttctcgccag cgattcggtg atgggttaca
     7561 acgaggtctc ctcctatgtt tccactctct tctacttcct ctcgacggag tacactctgg
     7621 cggaagcggg cgaggacggg cgcttcatcc cgggccgctc tgcccgcatc atggtggatg
     7681 ggaaggaggt cggcatcttc ggcgaaatcc atccccaggt gctggagaac tggaagagcg
     7741 acacgcccac cgtcgggtgc gagatcgacc ttgacacgct tctgggacac tgaacaccaa
     7801 gaatgcagtg gtatgttgtt cggatagagt caggcccggg attttcccgg gcctgacagt
     7861 caatctgtga atatcggttt attgtacaat cagcgtgaac gtcttcgaga aggagactcc
     7921 atcctcatcg gtcacggtga gggtgaacgg cagctcgctt ccgctcggaa cggaaccgtc
     7981 gatgacgaag gtgaacgagt ccgtcgaggt ccttcgacca aagaaatccg gatgatgatt
     8041 gctttcgttg ggccgaaggg tcctgcgttg cggttgagga tcgaggattc gaacaagacc
     8101 atgatcgcat tccaatgagg cggtgatgtc gttcatgacg aaatcgcccc gattacggta
     8161 agcgaaggac aagctgaggg cggtatttct gggaaccgca ttgctgttgt acgagtgcag
     8221 agcgatccgg gtgaagacga ggctcttata caccggcctg aagcaggcgc ctcttgtcgt
     8281 ccgctcgtag gtgtcttcgg taagaagggt atcgctttgg cgatcgtacc acccgacgaa
     8341 gacccggtcc tcgtcatcgt ccggaatggt aggaacggta accacccgat cggagctgta
     8401 gtactcgatt tcgtggatcg ggtcgtagaa gaggaccccc tcttcgtagg tcgccgtaag
     8461 ggatgtatcc ttatccggca tggtaatcgt ttctcccgct tcgtatgtgt tcttcccgtc
     8521 gctccaggat gcgaggaatc cgatctggcc cgggttgaag cggatttggc tgcgggaggg
     8581 caggcgaacc ggcgttcccg gtgcctgaat gatctgttgg gtataggagt accggtaggt
     8641 ctctccctcg gcggtgaagt caagggtgag ggtgaccggc gaacgccggg atgattcgac
     8701 gatgacagcg ggcgtttcct gaacctgttc gacaggttgc tccgaaaggg gggcggcgaa
     8761 cagcccgaag gttcctatgg tgaggacaaa cgcaaaaaga atggatcttt gaagtattct
     8821 catatggttg actccttgtt gctgatctac ccaaaaagat agcaaagcct tctctcgttg
     8881 acaagaatcg ccgggaaagg tacacacact ctccacaatc ggagcccggc ggtatcgtag
     8941 ccttgacttt ttttgcagca tcccaccccc aaataaggcc ataatcaatt gtcccggatg
     9001 aagttaccac ccgggacatc aaacatggga ttgtgttatg ttcaggagct ctgcttcacg
     9061 taggccaaca atgagcgcac ttcacctgcc gcggggtgtt gcccggagaa atcaattccg
     9121 agattcttgc cgattattct tcagaggctc atcaatcgga agatcctctc gcccacatcg
     9181 ctttttatct gactgtccgg atggcggatc cgaccattct ggcttgctct cgggacattg
     9241 aacaccaagc atgcggtgct caggaaggtt gtgttgttcg gatagagtca ggcccggggt
     9301 ttcccgggcc cgacattcaa tctatatgta tcgactcact aggg
//
LOCUS       DAOSLI010000060         9226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_7138_length_9226_cov_27.406281, whole genome shotgun sequence.
ACCESSION   DAOSLI010000060 DAOSLI010000000
VERSION     DAOSLI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7138_length_9226_cov_27.406281"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..893)
                     /locus_tag="PLF67_07810"
     CDS             complement(207..893)
                     /locus_tag="PLF67_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE89951.1"
                     /translation="MSDRKSDVELTFAQRIEVSLNRFFSKWGVVIAYISVVILIAAIA
                     LVSIGIYESKRLNKQFNEIDLLESDYMTFQSLGEDDESYAQKREELLLGLNALASKGK
                     GYPALKAQYLLGLFAFTEGSYREAIDHFNGVYQMGKNTYFQSLALANAAASAENMGDV
                     QQALEYYTRIIDEFGFEAAESPKALFAQGRLEEGRGNTDLAKATFQQLADQFPNSEYA
                     KLALNRIAFL"
     gene            complement(961..2679)
                     /locus_tag="PLF67_07815"
     CDS             complement(961..2679)
                     /locus_tag="PLF67_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOE89952.1"
                     /translation="MAEEQVLDEKRNLMQEALQEEYLKSLDGIEDGQLVAGTVVQINN
                     EYVFVDVGYKSEGRISTEEFDTMPEVGDEVKVIIVNKEGKGGQIIVSKRRADFKERTE
                     ELKAAAENRSPVLGKFEKVIKGGFEINLGGEYKGFCPLSKADVMRVEDPETLIGIEDY
                     FIIDKFHSGSRLKSVVNRREYLEMKIKENKEKFFSSVQIGDVVEGVVKSFTSFGAFID
                     LGGFDGLLHINDMSWGHVTRPKDFVKKGQVIQLRLINIDPETQKINLSLKHMQEDPWN
                     TFESRYKVDDVIKAAVTKLTTFGAFIEIEPGIEGLAHISELSWTRRINDPKEVLSVGD
                     VVEAKILGYDLDKKRVSLGLKQLDENPWDTITERYPVGMTLSKPVVKITNSGAFINLE
                     EGIDGFLHLDDISWTKKVKSMSSFCSEGDVIDVQVIRVEPDARRIRLGVKQLEGNPWQ
                     TLRSDYPKFSTISGTVTNVTDFGVFVKVPGEIEGLISKFNLVGPDEEYTDEVLKKFKV
                     GDPITAMVVELNPSTQKLSLSIKEMIKRSQQSEIAKYIHDEDDESDTFSLADMVRYKD
                     EEKDGN"
     gene            complement(2711..3355)
                     /gene="cmk"
                     /locus_tag="PLF67_07820"
     CDS             complement(2711..3355)
                     /gene="cmk"
                     /locus_tag="PLF67_07820"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOE89953.1"
                     /translation="MIVAIDGPAGVGKSSIARMLAEKSTFTYLNSGSFYRAYTYLHQQ
                     KGRDVGDLAGVLSTAKETHLRLVDGRLHADGIDIEDKLRTKEVDSVVAQVSAYPPLRS
                     YVNEQLKAIAQGEDMIVEGRDITTVVFPDAELKIYFDAKPEIRAERRFRQHPEGTTYE
                     EVLAGVKARDEIDRNKAVGALKQAEDALYIDTSYLTMEEVCEKVLSAIFALKMG"
     gene            complement(3352..4083)
                     /locus_tag="PLF67_07825"
     CDS             complement(3352..4083)
                     /locus_tag="PLF67_07825"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE89954.1"
                     /translation="MIEYPVRLQVYMAKSGCGSRRHCETLIAAGRVMVNMQRVTTLGT
                     KVTEDDVVMVDGRTVEPVEQYFYFALNKPRGYVSTNYDPNERNFARDLLDVPQKPLLF
                     SIGRLDKDSSGLLLFTNDGELTQKLSHPSHEVEKEYLVSASRPIAREDLEAMRRGVYL
                     PDNRLAYRIHRFEILGSRWTRIILTEGKNREIRKLLEHFDYRVLDLIRVRVGPVELGE
                     LKTGHWRRLTSDEVRRLHDEKGQKR"
     gene            complement(4080..4643)
                     /gene="scpB"
                     /locus_tag="PLF67_07830"
     CDS             complement(4080..4643)
                     /gene="scpB"
                     /locus_tag="PLF67_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOE89955.1"
                     /translation="MSDAIRSLSEDARLLEVILFLENEPVSVEKFVKMTDLNEDEVME
                     ALAEIEEYYDTYRHGMSLFENHGAFQFLPSADLHERLRSCYGRRVDRRLSRAAMETLS
                     IVAYSQPITRREIDNIRGVSSDSIIRLLRDREYIKVVGRKDVPGHPCLYGTSRKFLFE
                     FNLNSIAALPKLSEIDQERFEVNEEEA"
     gene            complement(4640..5584)
                     /locus_tag="PLF67_07835"
     CDS             complement(4640..5584)
                     /locus_tag="PLF67_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOE89956.1"
                     /translation="MERTEQQVSENLGLVYTTAGFEGPLGLLLHLIQKAEVNIYDIPI
                     AEITEQFIAYLESEESVLLGDLTEFYKMAADLLFIKSRMLLPVELEFDEEYQDPRQEL
                     VERLLEHQRFKKYTDLLSGTATSGELFISRKPSQFRLPFGDEELFGEVTLDDLLQTFR
                     KLIKQIAPSKVFNVYESVTVNEKIALMQEIFETREYMTIEELIVDPTSRLHIICAFMA
                     ILDATKQGMIYLLQERAFETIVIRRRDEYFEEDYSDAYDEDEEFIPAQIEPLPISNEE
                     EAAFYREDVIHGDDDERELIFDGDVEEIVFDDDEEDEL"
     gene            complement(5588..6634)
                     /gene="recA"
                     /locus_tag="PLF67_07840"
     CDS             complement(5588..6634)
                     /gene="recA"
                     /locus_tag="PLF67_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOE89957.1"
                     /translation="MAKSKDSSFPLDLKQKQDALEAARAQIDKQFGKGSLMKLGDADE
                     TLNIESIPSGSLLLDEALGIGGYPRGRIVEIYGPESSGKTTIALHAIAEAQKAGGIAA
                     FIDAEHAMDPSYAKKLGVNIDELWISQPDSGEQALEITESLVRSGAIDMIVIDSVAAL
                     TPQAEIDGDMGDSHMGLQARLMSQALRKLTGLLSKSNTTIIFINQIRMKIGVMFGNPE
                     TTTGGNALKFYSSVRIDVRKIETLSRGADDVYGNRIRIRIVKNKVAPPFKKVELDLLF
                     GEGISYEASLLDAALKYEMIDKAGSWYSYKDEKIGQGRDRTIEFLKSNPDILADLDKR
                     LRETMFGHSNVEKE"
     gene            complement(6685..7122)
                     /gene="dtd"
                     /locus_tag="PLF67_07845"
     CDS             complement(6685..7122)
                     /gene="dtd"
                     /locus_tag="PLF67_07845"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOE89958.1"
                     /translation="MRAVIQRVYDASVTVDGEVVGSIERGLLVYLGVGEGDTEEQLKW
                     ICEKIPKIRCFRDEHGKMNLDLKQVDGHILLVSQFTLLANLRKGNRPSYDRAADPKIA
                     EALYDKAFSLFEEQGVPVSGGVFGAHMHVRYTNDGPVTFTLDA"
     gene            complement(7119..7781)
                     /gene="rpe"
                     /locus_tag="PLF67_07850"
     CDS             complement(7119..7781)
                     /gene="rpe"
                     /locus_tag="PLF67_07850"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOE89959.1"
                     /translation="MSTPSLIIAPSMLASDFSRGAAEVASIKNHGADWVHLDVMDGAF
                     VPNISFGPVVIESLRKHSDLPFDTHLMIERPERYIADFAAAGSDYLTIHYEGNVHLDR
                     TLNLIKEHRVKAGISIVPSTPVDALKEILHLVDLVLVMSVNPGFGGQKMIPSTLKKIT
                     RLKDLREECGYTYLISVDGGVNLDTVSSIVEAGVDVAVTGSAFFGAEDRTRFVQRMKG
                     LP"
     gene            7931..9013
                     /gene="uxuA"
                     /locus_tag="PLF67_07855"
     CDS             7931..9013
                     /gene="uxuA"
                     /locus_tag="PLF67_07855"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HOE89960.1"
                     /translation="MLHMTLRWFGTNHDTVPLKYIRQIPGVEGVITTLYDIPAGEVWP
                     SERIRELKTEVEAAGLKILGIESVNIHDAIKIGSQDRERYIDNYIETLRNLGKEGITT
                     VCYNFMPVFDWTRTDLAKERPDGSTVFAYDEEIVRSIDPLTLFDHTTASSEGFLMPGW
                     EPERLANIKDLFEAYKEVDEKTLFDNLVYFLKRIRPVAEEYDIKMAIHPDDPVWPVFG
                     LPRIITGLDKVKKLMKAVDAPFNGVTFCTGSFGTNPKNDLPEMIRALEGRIHFAHIRN
                     LEHVEEGVFEESAHLSRDGSFDLYEIVKALHDIGFDGPVRPDHGRMIWDEVAMPGYGL
                     YDRALGAMYILGLEEAIVKNQEMKNA"
BASE COUNT         2117 a         2496 c         2285 g         2328 t
ORIGIN      
        1 aaggtcatcc aacgtgcttt gatccaccac atagttggcg gtaatcttcg tttcttcctt
       61 ggtctttacc ctgatcgagc tctgaattgt gaagatcgtg ggtattccgc acccgatgag
      121 cacaaaaaaa gcagatacga tgagaacaag ggtaagcctt ttcttcatgg tatctgcctc
      181 ctcaggggcc ttaaaatgct cgtcgtctag agaaatgcga ttcgattcaa ggccagtttt
      241 gcgtactcgg agtttgggaa ttggtcggca agctgttgga acgtcgcttt tgcgaggtcg
      301 gtattgcccc tgccctcttc aaggcgtccc tgagcaaata atgcttttgg cgattcagcc
      361 gcttcaaaac caaactcatc aatgatcctc gtgtaatatt caagggcttg ctgaacatca
      421 cccatattct cggcgctcgc cgccgcattg gcaagagcaa gactttggaa ataggtattc
      481 ttgcccattt gatacacacc gttgaagtga tcaatcgctt cccggtaaga accctcggtg
      541 aacgcaaata atccaagaag atactgtgct ttgagagccg gatatccctt gcctttggaa
      601 gcaagcgcat tgaggcccaa aagcaactcc tccctctttt gagcgtatga ttcatcatcc
      661 tcgccgagac tctggaaggt catataatcg ctttcaagga ggtctatttc attaaactgc
      721 ttattcagtc ttttggactc gtagatgccg atgctcacca aagcgattgc cgcaatgaga
      781 attaccactg aaatataggc gatgacaacg ccccatttcg agaaaaatct atttaaggaa
      841 acttcaattc gctgagcaaa ggtcagttcc acgtcacttt ttctatcact catatcctat
      901 ctccgtgttg ccaaggtatg cacaaaggcg gcctgagccg cctctgctgt aaagagggaa
      961 tcaattcccg tccttctctt catccttgta acgcaccata tctgcaaggg agaaggtatc
     1021 gctttcatca tcttcatcat ggatgtactt ggcgatctcc gattgctggg agcgcttgat
     1081 catctccttg atcgaaagag aaagcttctg agttgagggg ttcagctcga cgaccatcgc
     1141 ggtgatcgga tccccgacct tgaacttctt caagacctcg tcggtgtact cttcatccgg
     1201 accgacaaga ttgaacttgc tgatcaggcc ctcgatctca ccgggaactt tgacgaagac
     1261 gccgaaatcg gtgacgttcg taacggttcc gctgattgtc gagaacttcg gatagtcgct
     1321 tcggagcgtc tgccaagggt tgccctcaag ctgcttcacg ccgagacgga ttctgcgggc
     1381 atcaggttcg acccgaataa cctggacgtc aatcacgtcg ccttcgctgc agaaagagga
     1441 catcgacttc actttcttcg tccaggaaat atcatcgagg tggaggaatc cgtcgatccc
     1501 ctcttcaagg ttgatgaacg ccccgctgtt ggtgatcttc accaccggct tggaaagggt
     1561 catgccgacc ggatagcgct cggtgatcgt atcccaaggg ttctcatcca gctgtttgag
     1621 acccaaagaa acccgcttct tgtccaggtc atacccgagg atcttcgctt cgaccacgtc
     1681 gcctacgctc aagacctcct tcggatcgtt gattcggcga gtccaggaaa gctcgctgat
     1741 atgagccaac ccttcgatgc cgggctcgat ttcgatgaac gcaccgaagg tggtcagctt
     1801 cgtaaccgca gccttgatca catcatcgac tttataacgg gactcaaagg tgttccaggg
     1861 atcttcctgc atatgcttga gggaaaggtt gatcttctgc gtctccggat cgatgttgat
     1921 caggcgcagt tgaatgacct gccctttctt cacgaaatct ttgggtcggg taacatgccc
     1981 ccagctcatg tcgttgatat ggagcaaacc gtcaaagccg ccaaggtcaa tgaacgcacc
     2041 gaaggaagtg aaggatttga ccacgccttc gacaacgtca ccgatttgaa cggaggagaa
     2101 gaatttctcc ttgttctcct tgatcttcat ttccagatat tcacggcggt tcacgacgct
     2161 ctttaagcgg ctgccgctgt ggaatttatc aatgatgaaa taatcttcta tgccaataag
     2221 ggtttcgggg tcctcaacgc gcatgacgtc cgctttggag agcggacaaa accctttgta
     2281 ttcaccgcca aggttgatct caaatccacc cttgatgacc ttttcaaact tgcccaacac
     2341 gggtgagcgg ttttctgcgg ctgccttcag ctcttcagta cgctctttaa agtcagctcg
     2401 tcgcttggaa acaatgattt gtccaccctt gccttctttg ttgacgatga tgactttcac
     2461 ctcgtcaccg acttccggca tcgtgtcgaa ctcttcggta gagatcctac cctcgctctt
     2521 gtaaccaaca tcaacaaaca catactcgtt gtttatctgg acaacagtac cggctacgag
     2581 ttggccgtct tcgattccat caagagattt aagatactcc tcttgcagag cctcctgcat
     2641 taagtttctc ttttcatcca atacttgttc ttcagccact tccctttctc ctgatccaaa
     2701 atgtatctat ttatcccatt ttcagggcaa aaatagcaga taacactttc tcacaaacct
     2761 cttccatagt caagtatgat gtatcgatgt acagggcgtc ctcagcttgc tttaaggcac
     2821 ccacagcctt gtttcgatcg atttcatcgc gggctttgac cccggcaagc acctcctcat
     2881 aggtcgtccc ttcaggatgt tgcctgaagc ggcgttcggc tcgaatttcc ggctttgcgt
     2941 cgaagtagat cttcagttcg gcatcgggaa aaacaaccgt ggtgatatcc cgcccttcga
     3001 cgatcatgtc ttctccttga gcgatcgcct tcagctgttc attcacatag gagcgcagcg
     3061 gaggatatgc cgatacttga gcgacaaccg aatcgacttc cttggtccgc agcttgtctt
     3121 cgatatcgat cccatcggcg tggagacgtc cgtcgaccaa ccggaggtgc gtctctttgg
     3181 ccgtggaaag aactcctgca agatcgccga catccctccc cttttgttgg tgaagatagg
     3241 tataggcgcg ataaaaagaa ccgctgttca ggtacgtgaa ggtgcttttt tcagcaagca
     3301 tccgggcgat tgaggatttt ccgaccccgg cgggtccatc gattgccacg atcatcgttt
     3361 ttgtcctttt tcatcgtgaa ggcggcgaac ttcatccgaa gtaagtcggc gccagtgtcc
     3421 tgttttcagt tctcccaatt caaccggtcc aacgcgaacc ctaatcaggt ccagtacccg
     3481 gtaatcaaaa tgttcaagca atttccggat ttcacggttt ttcccctcgg tgagaatgat
     3541 tcgggtccat cgagacccca gaatctcaaa tcggtgtatc cggtaggcaa gccgattgtc
     3601 gggcagatat actccccggc gcatcgcttc cagatcctct cgggcgatcg gccgggaagc
     3661 gctgaccaag tactcctttt ccacttcatg ggaaggatgg ctcagcttct gagtcagctc
     3721 gccgtcgttg gtgaagagga gcagcccgct ggaatccttg tcgagcctgc caatgctgaa
     3781 cagcagcggt ttttgcggga catccagcag atctctggcg aagttccgtt cattgggatc
     3841 atagttggtg ctcacgtagc cgcggggttt gttgagggcg aagtagaaat attgctcaac
     3901 cggttcgacc gttcgaccgt cgaccatgac cacatcgtcc tcagtgactt tcgttccgag
     3961 ggtcgtgacc cgctgcatgt tcaccatcac cctccccgct gcgatgaggg tctcgcaatg
     4021 gcggcgggat ccacacccgc tttttgccat atagacctgg aggcgaaccg gatactcgat
     4081 catgcttcct cctcattgac ttcaaagcgt tcctgatcga tttcactcaa tttaggcaac
     4141 gctgcgatcg aattcaaatt gaattcaaac aaaaacttcc gactcgttcc atagaggcac
     4201 ggatggccgg ggacatcctt gcgtccgacg actttgatat actcccgatc acggagaagc
     4261 cgaatgatcg agtcgctgga aactccccgg atattatcga tttcgcggcg ggtgatcggc
     4321 tggctgtagg cgacgatcga gagcgtctcc atagcggccc ggctgagccg gcggtcgacc
     4381 cgtcgcccgt agcaggaacg aagccgttca tgaaggtccg ccgaaggcag gaactggaag
     4441 gctccatggt tttcgaagag actcatgcca tgccggtagg tatcgtaata ctcttcgatt
     4501 tcagctagag cttccatgac ttcatcttcg ttcagatcgg tcattttcac gaacttctcg
     4561 acgctgaccg gttcattttc caaaaacagg atcacctcca acaggcgagc gtcctcactg
     4621 agggatcgga ttgcatcact cacaattcat cctcctcatc atcatcaaac acgatttctt
     4681 ccacatctcc gtcaaatata agttcccgtt cgtcatcatc gccatggatc acatcctccc
     4741 tgtagaaagc ggcttcctcc tcattgctga tcggcagggg ctcgatctga gccgggatga
     4801 actcctcatc ctcgtcatac gcatcggagt aatcctcttc aaagtactcg tcccgtcttc
     4861 gaatgacgat cgtctcgaac gcccgctcct gcaggaggta gatcattccc tgtttggtgg
     4921 catccagaat cgccatgaag gcgcagatga tgtgaagtct gctggttgga tcgacaatga
     4981 gctcttcgat cgtcatatat tctctggttt caaagatttc ctgcatcaag gcgatctttt
     5041 cattgaccgt taccgactca tagacgttga acaccttcga cggggcgatc tgtttgatca
     5101 gttttcggaa cgtttgtaga agatcgtcaa gggtcacctc accgaagagc tcctcatccc
     5161 cgaacggcag gcggaactgg ctcggttttc gggagatgaa gagttcgccg ctcgtggcgg
     5221 ttccgctgag caggtcggtg tacttcttga agcgctggtg ctccaagagg cgttcgacca
     5281 gctcctgacg gggatcctga tactcttcat cgaactccag ttccaccgga aggagcattc
     5341 ggctctttat gaagagcaga tccgccgcca tcttgtagaa ttccgtcaga tcgccgagaa
     5401 gaaccgattc ctcgctctcc agataggcga tgaactgctc ggtgatctcg gcgatcggaa
     5461 tgtcgtagat gttgacttcg gctttctgaa tcagatgaag cagcaatccg agcggacctt
     5521 caaagcctgc ggttgtatac accaagccga gattctcaga aacctgctgc tccgttcgtt
     5581 ccacgtttta ctctttttcg acgtttgagt gcccaaacat cgtttccctg aggcgtttgt
     5641 ccaaatcagc gaggatatcg gggttcgatt ttaagaactc gatcgttcga tcccggcctt
     5701 ggccgatctt ctcatccttg taggaatacc acgatccggc tttgtcgatc atctcgtatt
     5761 tgagggcggc gtccagcagg ctcgcttcat agctgattcc ctccccgaag aggagatcca
     5821 gctccacctt cttgaacggc ggagccactt tgttcttcac aatccggatt cgaatccggt
     5881 ttccatatac gtcatccgct cccctgctga gggtttcgat cttgcggaca tcaatgcgga
     5941 ccgaggagta gaacttgagg gcgtttccgc cggtggtcgt ttcggggttc ccgaacatca
     6001 ccccgatctt cattcggatc tggttgatga agatgatcgt cgtgttggat ttgctcaaga
     6061 gaccggtgag tttgcggaga gcctggctca tcaggcgcgc ctgaagaccc atgtgactgt
     6121 cgcccatgtc tccgtcgatc tccgcctgag gggtaagagc ggccacgctg tcaatgacga
     6181 tcatgtcgat tgcgccgctg cggaccaggg actcggtgat ttcgagcgcc tgttcaccgc
     6241 tgtccggctg gctgatccag agctcgtcga tattcacccc gagcttcttc gcataggagg
     6301 gatccatcgc atgctcggca tcgatgaacg cggcgattcc tccagccttc tgcgcttcgg
     6361 cgattgcatg aagggcaatc gtggtttttc cgctggactc gggaccataa atctcaacga
     6421 tcctgccccg cggatacccg ccgatgccga gggcttcatc aagcaggagc gaaccggagg
     6481 ggatcgattc gatattcaac gtttcatcgg catcaccgag cttcatcagc gacccttttc
     6541 cgaactgctt gtctatttgg gctcgggccg cctcaagggc atcctgcttt tgcttcagat
     6601 ccaagggaaa cgaactgtct ttactttttg ccatggtaac tcctctggtt ccttatgggc
     6661 ggtatagtac catattgcat caacttatgc atcaagggta aaggtaaccg gaccatcatt
     6721 tgtatatctg acatgcatat gtgcgccaaa gactccaccg gagacgggaa cgccctgttc
     6781 ttcaaacagg gaaaacgctt tgtcgtacaa cgcttcggcg atcttcgggt ccgccgcccg
     6841 gtcgtaggag gggcggttgc ctttgcggag gtttgccagc agagtgaatt ggctgacgag
     6901 aagaatgtga ccatcgacct gtttcaggtc gaggttcatc ttgccgtgtt catcccgaaa
     6961 acagcggatc ttgggaattt tttcgcaaat ccacttcagc tgttcttccg tgtccccctc
     7021 gcccactccc agatatacga gcagaccccg ttcgatggag ccgacaacct cgccatcgac
     7081 agtgacactc gcgtcataga cgcgctgaat gaccgctctc acggaagacc cttcatccgt
     7141 tgaacgaacc gggtacggtc ttcggcgcca aaaaaggcgc tgccggtaac ggcgacgtcg
     7201 acacccgctt caacaatgga agaaacggtg tccagattaa ctcccccatc gacactgatg
     7261 aggtaggtgt agccgcactc ctctctcaaa tctttcaatc gggttatttt tttcaaggtg
     7321 gacgggatca tcttctgtcc cccgaagccg gggttcacgc tcatcaccaa aaccaagtca
     7381 acgagatgaa gaatctcctt caatgcatcg accggtgtgg agggaacgat gctgattccc
     7441 gctttgaccc gatgttcttt gatcagattc agggtgcgat ccagatggac gtttccttca
     7501 taatgaatcg tgaggtaatc actgccggcg gcggcgaaat cagcgatata acgctccggt
     7561 cgctcaatca tcagatgcgt gtcgaaggga aggtcgctgt gctttctgag gctctcgatg
     7621 acaaccggtc caaagctgat attcggaaca aaagccccgt ccatcacgtc aaggtgaacc
     7681 caatccgccc catggttctt aatcgaagcc acctcggccg ctcctctgga aaagtcgctg
     7741 gcaagcatgc tcggtgcaat aatcaatgag ggtgtgctca taccctaagt tataccaaga
     7801 agcaggccga tatgcaacga ttggttgagg aaagaatcga cgttgccacg ggtgtattcc
     7861 gccggttgaa gattgttgcc gatatgtaaa tatgctagcc tttgctcatt aaacgatagg
     7921 aaacataccc atgctccata tgacactgag atggtttggc accaaccatg acaccgttcc
     7981 gttgaaatat attcggcaga ttcccggtgt agagggggtc atcaccacct tgtacgacat
     8041 ccccgccggg gaagtatggc caagcgaacg cattcgcgag ctcaagaccg aggttgaagc
     8101 cgccggcttg aagattctcg gaatcgaaag tgtgaacatc cacgatgcga tcaagatcgg
     8161 tagccaagac cgggaacggt atattgacaa ctatatcgag acgttgagga acctcggtaa
     8221 agagggaatc actacggtgt gttataactt catgccggtt tttgactgga cccgcaccga
     8281 tctggccaaa gagcgccccg acggatcgac cgtttttgcc tacgacgagg agatcgtcag
     8341 atcgatcgat cccctgacct tgttcgatca tacgaccgcc tcgagcgagg gctttttgat
     8401 gccgggttgg gaacccgaac gccttgcaaa cataaaagat ctttttgaag cgtacaaaga
     8461 ggtcgatgaa aagactctct ttgacaacct cgtgtacttc ctgaagcgta tccggcccgt
     8521 tgcagaggaa tacgatatca agatggcgat tcatcccgac gatcccgtct ggccggtttt
     8581 cggattgccg cgaatcatta ccgggttgga caaggtcaaa aagctgatga aggcagtgga
     8641 cgcgccgttt aacggagtga cgttctgcac cggcagcttc gggaccaacc cgaaaaacga
     8701 cttgcccgaa atgatccggg ctcttgaagg ccgaatccac tttgcccaca tccggaacct
     8761 cgaacacgtt gaagagggag tctttgaaga atcggcccac ctttcccggg atggatcatt
     8821 cgacctgtat gaaatcgtca aagcgcttca tgacatcggt ttcgatgggc cggtccgccc
     8881 cgatcatggt cggatgatct gggacgaagt tgcgatgccc ggttatggct tgtatgaccg
     8941 ggctctcggt gccatgtaca tcctcgggct tgaagaagca attgtcaaaa accaagaaat
     9001 gaagaacgct taaccaaccg gcccctgttt ggaaaagacg taacaggaat caaagagaga
     9061 ccatttcata tcttgaatac atcgtttcaa tttcttccgg aattctctga tttgtgatac
     9121 gggaagctct attgagccga caaatcaatt gctaaaaact caaattttat gtatactttg
     9181 caagatagat agaaagaagt tttggactgg gcagacaaag acgaat
//
LOCUS       DAOSLI010000061         8977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_7344_length_8977_cov_20.917283, whole genome shotgun sequence.
ACCESSION   DAOSLI010000061 DAOSLI010000000
VERSION     DAOSLI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7344_length_8977_cov_20.917283"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..1196
                     /locus_tag="PLF67_07860"
     CDS             387..1196
                     /locus_tag="PLF67_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE89961.1"
                     /translation="MYWTFQCDKIGDLVTKGVDGSLTFSMDIDQQHFRYKFGKDTTKN
                     IYSLENDVEPRSSNSIFLPAKEVLSLHGIILKSRETDKTFGFDDTYVDLARALRQPPR
                     QGGNDRKLADARKHLRTIINGRVQMDEESGQWSFRKGNRKFAIGVTAEGVKKIAILDT
                     LLSNRYLDTRSIIFIDEPESALHPKAISEFMDIIVILAQSGIQFFLASHSYFVIKKLF
                     LLSQQHRMSIPVLSAEGQEWKSNDLIKGMPNNSIIDESISLYEEEVELSLL"
     gene            complement(1762..2496)
                     /locus_tag="PLF67_07865"
     CDS             complement(1762..2496)
                     /locus_tag="PLF67_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE89962.1"
                     /translation="MRIQIITLNLWNTEYWENRTNAVEAFLATYQADIYCFQEIREET
                     LVFLDARLPDYTRIEGSEPGWRSENTIYVRKTSFNVEAFGRVDLAMPETDRGLFWAKL
                     RTKKGIPLFVGTMHLTHQLNADECASGMSHRHRQAHRAAKALDSLVGTEAAVICGDFN
                     DPVHPSRILKSEAGFEDVFTILKVPAPVTFPCPYLSDETDLVESIDKIMIKGKIRPLL
                     ASSPRFSIPNSVLSDHWPVAAVLELE"
     gene            complement(2505..4553)
                     /locus_tag="PLF67_07870"
     CDS             complement(2505..4553)
                     /locus_tag="PLF67_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89963.1"
                     /translation="MQIRQISQNSVEILNPLHIVTITYTVTYEGKRQHPITLEIEDDT
                     VSASFEGIGTIEDRLTPNRNGVTVDRTWILQKEGSYRLQAGFFTTDEGVPNDLFIPAI
                     WYRDNTKGTGLFPSINRSSTWSFLETRMSIPCCLQLSAAKRHFLCATEAAAEERFLAS
                     VANDRHGAIISIPGWEWPYSYQGKKSLVDTASAPRPILSVPSGGLSYRRSFHLATYRE
                     MRDMEAYVAFVASLPKAKPVELPHPLSWNDWMELKLTRLINLVRRSEDGLAYIVMGEG
                     NGEVQDVYEFTGASFLVKSVQGAYELARFTTYEPTLECLQAARRALAKRFSLVDDSRL
                     LATVAKKIGDFFLKAERREGVFQDNHDLRTGQWGGYLGIGEFPEFAEMVNSRCNGEAM
                     NHYLRLAKELQSLGYDVSAYIDLARRVSLFYSDIQLSSGSFGRWWTVKGKPGDTLGTN
                     GAYIGSFLCALIPLLEESDPLKAVLIGAVHKAYMFYAQLADEGAFYGDTLDADSCDKE
                     AGVALLSFFMDLYHLEGDKRHLTSAHLAAEFIIQWIWQQSSYLPGESPLGKKGFSTLG
                     MTSVSVAHHHLDCYGMAIAYEFLRFAEAANLPFYAEQASLMIAACKQLVHGKENDLGR
                     DESFFGWQPEQVNHTSWEYFNRPELMNGHYEIDIAWVTILTLSSFDRIRGEFPEVLQE
                     "
     gene            complement(4558..5385)
                     /locus_tag="PLF67_07875"
     CDS             complement(4558..5385)
                     /locus_tag="PLF67_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE89964.1"
                     /translation="MKAQSTSKKILFRIIELLFIFAWMLPLIWMAITSLKLEHEVITK
                     TFTFLPKHPTLANYTKAFTSTYILRWLYNSVIVSTLAMVFTLVLDAPIAYAFAKIDFK
                     GRNVLFWAVMGGMMVPFQVLIVPLYLQFNTYGLVNSLAAVYLPRLALPIGIFILKQFY
                     EGIPGELEEAAFIDGAHRFRIFLRIILPLGQSAMATVIILSFINTWNDFLWPLIVVND
                     TIKYPITVGIANFQGTHGTQYSLIMAGALVASVPQIIFYIFFRKKIIAGIATSGIKG"
     gene            complement(5382..6254)
                     /locus_tag="PLF67_07880"
     CDS             complement(5382..6254)
                     /locus_tag="PLF67_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE89965.1"
                     /translation="MIPGNKDTRDGLLFSIPFLTVYLLFMIYPLFHGLYISFFKWDIL
                     TKPSFIGLANYKTLFQDATFYSSLWHTIQFVLMTTPTLLVIGFLMALAITGSSPLKSV
                     MENTFFFPYIFSMTVVSTLWAWLMQKDFGLFNQTLTLIGVRPISWLTNEKYAMWSIAL
                     TTLWWTSGFNMVLFGAGIKQIPKEVYESASIDGATYWQSVRHITIPLVSSTTILCLIL
                     QIIASFNVFGQVYVMTGGGPHGTTRVLVQYIFETGFRYYKMGYSAALSYILFLIILVI
                     SVLQYVLLGRKEES"
     gene            complement(6308..7567)
                     /locus_tag="PLF67_07885"
     CDS             complement(6308..7567)
                     /locus_tag="PLF67_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE89966.1"
                     /translation="MKKRLFVLMVVLLISTLLFAQGAGEKKKDGPIEITFWSLFTGGD
                     GEFFDAMVDAFNASQNEIFMKNDMVKFDNYYVKLTTALAAKNAPDVVVVHQGNLLNYV
                     PSGSLLALDEYVKPGMLADFQTAPLEACRFDGKLYSLPFDVHPIVFYYNKDLMAKAGV
                     TKVPVTAEEFIEAALKIKKATGKWGLAIDNTTGVYKAYTLARLFMSMIAQQGGSLLNA
                     DATAPTFNNAYGERALTWLQGLVHDYKVNPPELDYDAAMNVFKLGDAAFYFNGVWATG
                     TLEATPGLNFGASQLPALMGKEAAWAGSHTLAIPVQRTLDPKRIEAAMTFIDWMTSHG
                     EMWAKAGHIPTRKSVALKDEMKALPYRSDYAAGAASALPTPRTPAWEEIYGTLSDMLE
                     YAVTKNQNPKQALAEMEKTVKEIIATY"
     gene            complement(7606..8604)
                     /locus_tag="PLF67_07890"
     CDS             complement(7606..8604)
                     /locus_tag="PLF67_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE89967.1"
                     /translation="MSVLLKDIAKATGFSINTVSRAMRSDPHISEKTTSLIKKTAKDM
                     GYIPNSVAASMRSNTSKMVGIISADSANPFFSEVVRGIEEAASKAEYHILLANTEESI
                     KKEAELVNMFLRRKVDGIIAMPVFDNSGSHLDLYQRLKIPFIFPGRRLEGLEGHSILH
                     SDRIGQRMVIDHLLDHGHTKILYIAGPRKVSNTIDRLSGVHEAFASHGMETDPDYIVE
                     ASGHIEDGYSISNQALNRGMGFTAIACFNDMLAMGVLKSLYENNLRVPEDIEVFGYDN
                     LYMSQFMQPSLSTVDVPKFRLGYVAMETLIAHIRDPELAYETKDFPTRLVFRETTR"
     gene            complement(8694..>8977)
                     /locus_tag="PLF67_07895"
     CDS             complement(8694..>8977)
                     /locus_tag="PLF67_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HOE89968.1"
                     /translation="DPLRDDGVLYADALASADTPVKYLEVKKGLHGFFAYPKATGTDE
                     AQSAITQFIRGKPVEQVALIGKKEWLKAEKLELKKIKKQAKHYIETEIG"
BASE COUNT         2143 a         2249 c         2478 g         2107 t
ORIGIN      
        1 tgagtgaacg ggtgaaagca atggccgaac gaaaaggcct catcttcttc gatgcagcga
       61 cggtagccac ccccagtgag atcgaccagc tccacatgga cgccgaaagc caccgaaacc
      121 tggccgaggc gttgaaagag gtgatcatcc ggcatctctg atccgtgaaa caaggagcaa
      181 tcatgttgga tcaggtatac atgcaaaatt atggatcact ccagaatctg agctggtcgg
      241 gtctcggtcc gatcaatctt gtccttggag aaaatggtgc cgggaagacg tttctcctga
      301 aagctctcta taccgcaata agaactcttg aagaatatcg acggggagac gagtagcgcc
      361 cgatagcgga aatccttgca caaaagttgt actggacgtt ccagtgcgat aagatcggtg
      421 atctggtcac aaaaggggtt gacggttcgc ttaccttttc aatggatatc gaccagcagc
      481 atttcaggta taaattcggc aaggatacaa ccaagaatat ttattctctg gaaaatgatg
      541 tcgaacctcg gtcaagcaac tcaatcttcc tgcccgcaaa ggaagtcctc tctcttcatg
      601 ggatcatcct gaaatcgagg gagacggaca aaacattcgg ttttgacgat acctatgtgg
      661 atctggccag agctctcaga caacctccaa ggcaaggagg gaatgataga aagcttgccg
      721 atgcccgaaa gcatcttcgt accatcatca atggacgggt tcagatggat gaggaatccg
      781 gtcagtggag ctttaggaaa ggcaatagga agtttgcaat cggggttacc gccgaggggg
      841 tgaaaaagat cgcaattctg gacaccttgt tgagcaatcg gtatcttgat acccgctcga
      901 ttattttcat agatgaaccg gagtcggcgc ttcatcccaa ggcgatttca gagtttatgg
      961 atatcattgt gattctggca caaagcggaa tccaattttt ccttgccagc cactcatact
     1021 ttgtaatcaa gaagttgttt ctcctttcac aacaacatag aatgtcgatt cccgttctct
     1081 cagctgaagg tcaagagtgg aagagcaatg acctcatcaa aggaatgccg aacaactcga
     1141 tcattgatga atccatcagt ctatacgaag aagaagtgga gttgtcgttg ttatgagcat
     1201 aacgatcaac gagtcaggaa taacgttcgg cccgtacgcg gaatcggtat gttttcaaat
     1261 tgaaaaaagt cccatataca cacatctagg gaaggatgtg aagatggttg aattcgtact
     1321 caatcgatta actgccggtg gtgagcaggt tttctgtttg gtggaggcga agtaaagttc
     1381 cccgcagccg gacaatccaa ttggttccct acattcatca gtgaaatcag tgaaaagatg
     1441 atacatggta tttcgctact tctcgcaatc cttttgaatc gacacgggtc aagtgaagaa
     1501 atcttacccg aatccatgcg ctctgttgat ctttcccgat gtaaggcgcg gtatatcctc
     1561 gtcatcaatg gacataagga agagtggcta cctccgatac aagacgcatt gcgtaaatcg
     1621 ttgcgatcaa tggacacgac atggaatttt ggaccgaata cggttgtggt attaaatgac
     1681 gagaaggctg ccggttatcc gttattgcac cccacgcagc gaaccgaagc cggagacggc
     1741 aactcatgaa agccgacatc actactccag ctcaagaacc gcggccaccg gccaatgatc
     1801 gcttaggacg ctgttcggaa tcgagaagcg cggacttgaa gcaagtagcg ggcgaatttt
     1861 tcctttgatc atgatcttgt cgatcgattc gacgagatcc gtttcatcgc tcaaataggg
     1921 gcaagggaac gtaaccggtg ccggaacctt gaggatggtg aagacatcct cgaagcccgc
     1981 ttcggacttg aggatccttg aagggtggac gggatcgttg aagtctccgc agatgaccgc
     2041 cgcttcggtt ccgaccagag agtcgagggc tttggctgcg cggtgtgctt gtcgatggcg
     2101 atggctcatt cctgaagcac attcatccgc attgagctgg tgggtcaggt gcatcgtccc
     2161 gacgaacagc gggattccct tcttcgtacg gagcttggcc cagaacagtc cacgatcggt
     2221 ttccggcatc gcgaggtcga cccggccgaa cgcttcgacg ttgaaagaag tcttccgtac
     2281 atagatcgtg ttctcgcttc gccaacccgg ctcgcttcct tcaatgcgcg tgtagtccgg
     2341 cagacgggca tccagaaaga caagagtctc ctcccggatc tcctgaaagc aatagatatc
     2401 cgcctgatag gtcgccagaa acgcttcgac ggcgttcgtc cggttctccc aatactccgt
     2461 gttccagaga ttcaacgtga tgatctggat ccgcatcgtc gacttcactc ctgcaatact
     2521 tcgggaaact cgcctctgat ccggtcgaac gaggagagcg tcagaattgt cacccaggcg
     2581 atatcgatct cgtagtgtcc gttcatcagc tccgggcgat tgaaatactc ccagctcgtg
     2641 tggttgacct gctccggctg ccagccgaag aaggactcgt cccggccgag gtcgttctct
     2701 ttgccgtgaa ccagctgttt gcatgctgcg atcatcaagg aggcctgctc cgcgtagaag
     2761 gggaggtttg cggcctcggc aaagcgcagg aactcgtagg cgatcgccat tccgtaacaa
     2821 tccaggtggt gatgggcgac cgagacgctc gtcatcccca aggtggaaaa acctttcttt
     2881 cccaagggac tctccccggg aaggtaggag gattgctgcc agatccattg gatgatgaac
     2941 tcggctgcaa ggtgggcgct tgtaagatgc cgcttgtctc cttccaggtg gtacaaatcc
     3001 atgaagaagg agagcagggc cacccccgcc tccttgtcac aggaatcggc atccagcgta
     3061 tcgccgtaga acgccccttc atcggcgagc tgcgcataga acatgtacgc tttatgaacc
     3121 gcaccgatga gaaccgcctt gagcggatcg ctttcctcaa gcaacgggat gagggcgcac
     3181 agaaacgatc cgatatacgc tccgttggtt cccagagtgt cccccggttt ccccttcacg
     3241 gtccaccagc ggccgaagga accggaggaa agctggatgt cggaatagaa gagggagacc
     3301 cttcgggcga ggtcgatata cgccgaaaca tcatacccga gcgattgcag ctcctttgca
     3361 agccggagat agtggttcat cgcttctccg ttgcaccggc tgttcaccat ctcggcgaac
     3421 tcggggaact ccccgatccc gaggtatcct ccccattggc ccgtccgaag gtcatggttg
     3481 tcctgaaaca ccccttcccg gcgttccgcc ttgaggaaga aatctccgat cttcttcgcc
     3541 acggtcgcca gcaggcggga atcgtcgacc aagctgaaac gcttggccaa tgcccgtcgt
     3601 gccgcttgca ggcactcgag ggtgggttca taggtggtga accgggcgag ctcataggcg
     3661 ccttggacgc ttttcaccag gaaggaggcg cccgtgaact catacacatc ctgaacctcc
     3721 ccgttgcctt cccccatgac gatgtacgca agaccgtctt cgcttcttcg tacgaggttg
     3781 atcaggcggg tgagcttcag ctccatccaa tcgttccagg aaagggggtg gggaagctcc
     3841 accggtttcg ctttcggaag ggaggcgacg aaggcgacat acgcctccat gtcgcgcatc
     3901 tcacgatagg ttgccagatg gaaggagcgt cgatagctca gaccgccgga agggacggaa
     3961 agaatagggc ggggagccga ggcggtgtcg acgagggatt tcttgccctg atagctgtat
     4021 ggccactccc atcccggaat ggagatgatc gccccgtggc ggtcattggc caccgaagca
     4081 aggaagcgct cctcggccgc cgcctcggtg gcgcagagaa agtgtcgttt tgcagccgag
     4141 agctggagac aacagggaat cgacatcctt gtttccagaa atgaccatgt tgaggagcga
     4201 ttgatcgacg ggaacaaccc ggttcccttg gtgttgtccc gataccagat tgcaggaatg
     4261 aagaggtcgt ttggaactcc ttcatcggtg gtgaaaaacc ccgcctgaag ccgatatgat
     4321 ccctctttct ggagaatcca cgtccggtcg accgttacgc cgttccggtt gggagtcaag
     4381 cgatcctcga tcgtcccgat tccttcaaat gatgcggaca ccgtgtcatc ttcaatctca
     4441 agggtgatcg ggtgttggcg tttaccttcg taggtgacgg tataggtgat cgtgacgata
     4501 tgcagaggat tgagaatttc aacggagttt tgggatattt ggcgaatctg cactgtgtta
     4561 acctttgatt ccgctggtag cgatcccagc gatgattttc ttcctgaaga agatgtagaa
     4621 gatgatttgc ggtaccgaag cgaccaaggc tcccgccatg atcagcgagt actgggttcc
     4681 gtgggttccc tgaaagttcg cgataccgac ggtgatcgga tacttgatcg tatcgttgac
     4741 cacgatcagt ggccagagga aatcgttcca cgtattgatg aacgagagga tgatcaccgt
     4801 ggccatcgcc gattgtccca agggcaggat gatccgcaag aagattctga agcgatgggc
     4861 tccgtcgatg aaggccgcct cttcaagctc gccggggatt ccctcgtaga actgcttgag
     4921 aatgaagatt ccgattggaa gggcgagccg ggggaggtat accgccgcaa gggagttgac
     4981 cagaccatac gtattgaact ggaggtagag cgggacgatg agaacttgga agggtaccat
     5041 cattcccccc atcaccgccc agaacaggac gtttcgtcct ttgaagtcga tcttggcgaa
     5101 cgcgtaggcg atcggggcat caaggacgag ggtgaagacc atcgccaacg ttgaaacgat
     5161 cacgctgttg tagagccatc tgaggatgta ggtggaagtg aacgccttgg tgtagttcgc
     5221 gagggtcggg tgtttcggaa ggaaggtgaa ggtcttcgta atcacttcat gttcgagttt
     5281 caggctggtg atcgccatcc agatcagcgg caacatccat gcaaagatga agagaagctc
     5341 aatgattcga aagagaatct tctttgaggt tgattgggct ttcatgactc ctccttccgg
     5401 ccgaggagga cgtactgaag cacactgatc accaagatga tgaggaagag gatatagctc
     5461 agcgccgcgc tataacccat cttgtaatat ctgaaacccg tctcgaagat atattggaca
     5521 agaactcgag tagttccatg aggaccgcct ccggtcatca cgtatacctg tccaaacaca
     5581 ttaaaggacg cgatgatctg caagatcaaa caaaggatcg ttgttgatga aacaagggga
     5641 atcgtgatgt gacggacgct ttgccaatag gtagccccat caatcgaggc ggattcatac
     5701 acctccttag gaatctgttt tattcccgct ccaaagagaa ccatgttgaa cccggaggtc
     5761 caccacagcg tggtgagggc gattgaccac atcgcatatt tctcattggt caaccagctg
     5821 atcggccgaa ccccgatgag ggtgagagtc tgattgaaga ggccgaagtc tttttgcatc
     5881 aaccatgccc aaagggtact gacgaccgtc atcgaaaaga tgtaggggaa gaagaatgtg
     5941 ttttccatca cgcttttgag tggcgaggat ccggtaatcg caagagccat cagaaaaccg
     6001 attacaagga gggtcggtgt cgtcatcaag acgaattgaa tcgtgtgcca caacgaggag
     6061 tagaaagtgg catcctgaaa aagggtcttg tagtttgcca gcccgataaa ggatggtttg
     6121 gtcaaaatat cccacttgaa aaagctgata tataagccat ggaagagagg gtagatcatg
     6181 aagaggaggt agacagtcaa gaaagggata gaaaagagca ggccgtcacg agtgtctttg
     6241 ttacctggta tcataggttg ctccaagcaa atgtcctcct ctcatctgag aggaggactc
     6301 gaaaggatta atacgttgca atgatttctt tgaccgtttt ctccatctcc gcaagggctt
     6361 gcttcggatt ctggttcttg gtcacggcgt actcaagcat gtcgctcaac gttccgtaga
     6421 tctcctccca cgcaggagtt cgaggagtcg gaagcgcgct tgccgctccc gctgcatagt
     6481 cggatcgata cgggagggct ttcatctcat ccttgagggc gacgctcttt cgggtgggga
     6541 tatgtccggc tttcgcccac atctcaccat gggaggtcat ccagtcgatg aaggtcattg
     6601 ccgcttcgat ccgcttagga tcaagggttc gttggacggg aatcgccagc gtgtgcgacc
     6661 ctgcccaggc ggcctcttta cccatcaacg cgggaagctg agatgcgccg aagttcagtc
     6721 cgggggtcgc ctccaaggta ccggtcgccc atactccgtt gaagtagaat gccgcatcgc
     6781 caagcttgaa gacgttcatg gcagcatcgt agtcgagttc gggggggttc accttatagt
     6841 cgtggacaag cccctgtagc caggtgagcg ctcgctcacc gtatgcgttg ttgaaggtgg
     6901 gtgccgtcgc atcggcgttg agcagagaac ctccttgctg ggcgatcatc gacatgaaga
     6961 ggcgggcgag ggtgtaggct ttgtagacac cggtagtgtt atcgatcgcc aagccccatt
     7021 ttccggtcgc tttcttgatc ttgagagccg cctcgatgaa ctcttcggcg gttaccggaa
     7081 ccttcgttac gcctgcttta gccatcaaat ccttgttgta gtagaagacg atcgggtgga
     7141 cgtcaaaggg aagactgtag agttttccgt caaagcggca tgcttcgagc ggggcagttt
     7201 ggaaatctgc gagcatcccc ggtttcacat actcgtccag tgcgagcagg gaaccgcttg
     7261 gaacatagtt gagcaagttt ccttggtgca cgacgacaac atccggagcg ttcttcgccg
     7321 ccagagcggt tgtgagcttg acgtaataat tgtcgaattt gaccatgtca ttcttcatga
     7381 agatctcatt ctgagaagcg ttgaacgcat cgaccatcgc gtcaaagaat tcgccgtcac
     7441 cgcctgtgaa gagggaccag aacgtaattt ctatcggtcc gtctttcttc ttttcaccgg
     7501 caccttgagc aaaaagaagc gtgctgatga gaagtactac catgagtaca aacaaacgtt
     7561 ttttcattgt gaacctcctt ttaggcacaa agtagtatgc tcttcctaac gggtggtttc
     7621 cctgaaaacc aacctcgtgg ggaaatcctt cgtctcatag gccagctcgg gatcccggat
     7681 atgagctatg agcgtttcca tggcgacata cccaagccgg aacttcggta catccaccgt
     7741 acttagactg ggttgcatga attgagacat gtagaggttg tcgtacccga agacctcgat
     7801 atcctcgggt actcggagat tgttttcgta caggcttttg agaaccccca tcgcaagcat
     7861 atcgttgaag caggcaatgg cggtgaaccc catccctcga ttgagagctt ggttcgagat
     7921 cgaatagccg tcttcgatgt ggccgctcgc ttcgacgatg taatcgggat cggtctccat
     7981 tccatgggaa gcaaacgcct cgtggacacc gctgaggcga tcaatcgtgt tgctcacttt
     8041 cctcggtccg gcgatataga gaatcttcgt atgaccgtga tcaagtaagt gatcgatgac
     8101 cattctttga cctattcggt cgctgtggag gatgctgtgt ccctcaaggc cttcgaggcg
     8161 gcgacctgga aagatgaagg gaatcttgag gcgttgatag agatcaagat gggagccgct
     8221 gttgtcaaag accggcattg cgatgattcc gtccactttt cgccgcagga acatattgac
     8281 cagttccgct tctttcttga ttgattcttc ggtatttgct agaagaatgt ggtattccgc
     8341 tttggaggcc gcttcttcta ttccgcggac gacttcactg aaaaaggggt ttgccgaatc
     8401 ggcactgatg atgccgacca tcttggaggt gttggagcgc atgcttgcgg cgacggagtt
     8461 cggaatatag cccatatcct tcgcggtctt cttgatcaaa gaggtcgtct tctcgctgat
     8521 atgagggtcg gagcgcatag ctcgcgagac ggtgttgatc gagaaaccgg tcgccttggc
     8581 gatatctttc agaagcacgc tcataggtta aggttaacat gaggaagtgg ggtatcaagg
     8641 taaatcccct agattatctg ttaaaaccaa caatattcaa gattaacgtt aatctaacct
     8701 atctcggttt cgatgtaatg cttcgcttgc ttcttgatct tcttcagctc gagcttttcc
     8761 gccttgagcc actctttctt tccgatgagg gctacctgct cgacgggctt gcccctgatg
     8821 aactgggtga tcgcgctctg ggcctcatcc gtaccggttg ctttcggata ggcgaagaag
     8881 ccgtggagcc ctttcttcac ctcaaggtat ttgaccggcg tatccgccga agcgagcgca
     8941 tcggcataca gaacgccgtc atcccgaagc ggatcgt
//
LOCUS       DAOSLI010000062         8852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_7451_length_8852_cov_18.495737, whole genome shotgun sequence.
ACCESSION   DAOSLI010000062 DAOSLI010000000
VERSION     DAOSLI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_8852_cov_18.495737"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLF67_07900"
     CDS             complement(<1..598)
                     /locus_tag="PLF67_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE89969.1"
                     /translation="MSPINDREVVLDVRDLKTYFKTDAGIVKAVDGVSFTLRKGQTLG
                     IVGESGSGKSVTNLSIMRLIPSPPGKVVGGEAIFEGTDILALDEKEMRTIRGNKISMI
                     FQDPMTSLNPFLRISTQMIETIRLHTKGISKMEALERSVEMLKLVGIPSAEQRVHAYP
                     HQFSGGMRQRVMIAMALSCNAEILIADEPTTALDVTIQA"
     gene            complement(611..2200)
                     /locus_tag="PLF67_07905"
     CDS             complement(611..2200)
                     /locus_tag="PLF67_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE89970.1"
                     /translation="MKNVVTTNEFNQVVEGNSLGKDAWRRLKKNKMAVVGMIIVILYS
                     ILAATAPLLPIHPYDQIILDHQHLRPSLTRKAGEIMMENRLKDLYFKAWRSGELVVTE
                     AQSAQIRKWIAANDTNKVWNFVLKEGEAQREAGTFVFNAADQRVITRLQEKIDTEFLI
                     NVTKITFSEEKGGKREDLNKMSYEEISRIYANLTKTPIEAVEAQTLTEVTSQLMNTLK
                     STTPGLSAEEYEVNLELELQTIGEGGIAKLGKTNLLGKISTTITRQADRDLKKEIKEG
                     LAVFPVERIIDVNEQLQAKVTATKKHERMYYLGTDYSGRDMLSRIIYGSQVSIAIGLV
                     GTLTSVLIGIVLGAIAGYVGGKVDYILMRIVDIMYGLPYMLLVIIFMAIFGRSILNLF
                     GALALVSWLTIARMVRGQVMSLKNSEYVEAARSMGGSTARIILRHMIPNSLSVIIVYS
                     TLRVPAFIMQESFLSFLGLGVQAPFASWGSLVGDAVDGMTLYPWRLIFPAIAMTLFLF
                     AMNFFGDGLRDAFDPQSKNEL"
     gene            complement(2212..3141)
                     /gene="oppB"
                     /locus_tag="PLF67_07910"
     CDS             complement(2212..3141)
                     /gene="oppB"
                     /locus_tag="PLF67_07910"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HOE89971.1"
                     /translation="MTKYIVNRLLGMIPTLFIIITLSFFIVRIAPGGPLSAERNLTDV
                     VRRNIEAKYHLDEPLIKQYGRYMFDIMRGDLGPSFKYKDYDVNYYIFTSLPKSIVLGL
                     WAMLISVTIGMGIGIIAAVRQNSWIDYLSMGLAVIGISVPLFVIAPVLQLIFAVKLKW
                     LPTSGWYTTGEGYLTIILPAVSLSFEYFANIARLTRSSMLETLRSDYIRTAKAKGMKR
                     STIIFKHAMKGALLPVVSYLGPAFAGIITGSVVVEQIFRVPGLGKFFVQSSFNRDYTL
                     IVGVVIVYSVILIVMNFIVDIIYAQLDPRITYK"
     gene            3303..4658
                     /gene="rimO"
                     /locus_tag="PLF67_07915"
     CDS             3303..4658
                     /gene="rimO"
                     /locus_tag="PLF67_07915"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOE89972.1"
                     /translation="MKTVYLENLGCSKNQVDAEVMLTLLQDEFSRTEDPAAADLIIVN
                     TCGFIESARAEAIDSFFDLYGSNPEAKIIFSGCMAQRYADELSAELTEASAIFGNRDL
                     SKLPEVVGQVFSGERVVSTPEYPANSFEDVYKRSRLLSFDGSAYVKISEGCNHRCSYC
                     AIPIIRGSLRSKPMAIILEEVTSLIARGVKEINLIGQDLASWGRDLKEADAHFMALLK
                     RIAEVEGDFVIRLLYIHPDTFPMELLEFIRNHPKILPYFDIPFQHATESILRSMGRRG
                     TKESHLALTRKIREMLPDAVIRSTILLGYPGEDEDSFAEVLDFLIQAQLDWVGSFVYS
                     LEEGTKAAELTNERAHRKLASKARRFQQELEALQLSITQRRLETFVGREVDVLIEEHV
                     EGEELYIGRTYAQAPEVDGLTVVMGRDLVPGTVVRCGITRVNGIDFEAVPLHGGYDGS
                     H"
     gene            4645..6873
                     /locus_tag="PLF67_07920"
     CDS             4645..6873
                     /locus_tag="PLF67_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE89973.1"
                     /translation="MAPIETYLKQLNEPQREAVLENSRPLLMLAGAGSGKTRVITTKI
                     AYAIEELKIPPYKILAVTFTNKAANEMKERVEAMLPDNPDVKECNIRTFHSFGAWLLR
                     RFGSEIGLENNFTIYDDDDSLSLLASIYPSYKKRELEPIMKRISHAKDRMLSPGDAMK
                     RFHADLLFKQMYERYEEKLRAVGNLDFADLIGRSIELLRDAPHVQEWIHSRFAMILVD
                     EYQDSNTAQFLLLKLLVGPETFVGVVGDDDQSIYRFRGAEVKNILTFASSFPKARTIK
                     LEQNYRSTTQILDVANSIISKNADRHDKNLWSARKGGRKPKLFYVHDEEDEARRIVTL
                     LREDRNYNSSAVLYRTNAQSVPFETVFKRYGIPYKVVGALQFYEREEVKDALALLYLL
                     TNPKDEVSFRRIINKPARGIGPGSVGKIIGFAPQTGGDLLQAAASAVQNRTLSPKAAQ
                     GAELAVRLIDEGRKLIVDGNLVDGVTFLINESGLYEYYRELDLLNTTQKVANLEALVN
                     AISVYEPSFDGLVAFLEQLTLDRTTLGTDDPRDRDGVTLITMHNTKGLEFDRVFVTGL
                     EEGLFPGRKNEREEDIQEERRIFYVAVTRARNELYLFSSKVRKIWGKTSFQNPSRFID
                     DIDPSLIQVEGSPAPQGFDSFAKRDAVSTDWGSSGSGMIRQGFAPGAKRFEMKVKHEK
                     QGDSPLFPVGQKVYSDANGEGEVVKIRGEGDREAIDVVFSTGKKATYLSRFADLDRIG
                     GE"
     gene            7228..7656
                     /gene="rplM"
                     /locus_tag="PLF67_07925"
     CDS             7228..7656
                     /gene="rplM"
                     /locus_tag="PLF67_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOE89974.1"
                     /translation="MKTIFVKPLTIQKKWYLIDAEGKELGRVAVAAARILRGKNKAEY
                     APLHDLGDYVIIINAEKAILTGNKYTDKMYYSHSGFPGGLRKTSYADMIKRKPTFPME
                     HAVKGMLPKGPLGRKLFTNMRVYAGAEHKQSAQQPIPVEI"
     gene            7671..8084
                     /gene="rpsI"
                     /locus_tag="PLF67_07930"
     CDS             7671..8084
                     /gene="rpsI"
                     /locus_tag="PLF67_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOE89975.1"
                     /translation="MAKKEATKAVNLGHGVGRRKTAVARVYLREGEGAIMINGRDVDS
                     YFGNPMLTFIVKQPLAATETDGKYDILINVKGGGVSGQAGACRHGIARALAIHDEAFR
                     PVLRAAGFMTRDSRMAERKKYGQPGARRKFQFSKR"
     gene            8086..8697
                     /locus_tag="PLF67_07935"
     CDS             8086..8697
                     /locus_tag="PLF67_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOE89976.1"
                     /translation="MSYARIVREPGKGGYQLLPSQGSPFYISPDVFRTLGLRVDQEVD
                     EELAAKLFRESQKQGCYAQGLSYLARSEHTTFHLVQKLTRKGYPKEIIDDAVSRLTEE
                     GALSDLRYARLFIERRQRKNPEGRLRLAQRLADRGVRNDAALQALDELFTEEHIATYV
                     ARALNRAKTGYPEKNVRDLLRLWGFSSYEIRTGLELAGDDELP"
BASE COUNT         1916 a         2404 c         2607 g         1925 t
ORIGIN      
        1 gggcctgaat cgttacatcc agcgcggtgg tcggttcgtc ggcgatgaga atctcggcgt
       61 tgcaggagag cgccatcgcg atcatcaccc gctgacgcat cccgccggag aactggtggg
      121 ggtaggcgtg aacccgctgc tccgccgacg gtattcccac gagcttgagc atttcgacgg
      181 agcgctcaag cgcttccatt ttgctgatgc ctttcgtgtg gagccggatc gtctcaatca
      241 tctgcgttga aatcctcaaa aaggggttca ggctcgtcat cggatcctgg aagatcatgg
      301 agatcttgtt gccccggatc gtccgcatct ctttctcgtc cagggcaagg atgtccgtgc
      361 cctcgaagat cgcctctccg ccgaccacct ttcccggagg ggaggggatc aggcgcatga
      421 tcgaaaggtt cgtcacgctt tttccgcttc cgctttcgcc gacgattccc agagtctgtc
      481 ctttacgtag ggtgaaggag accccgtcga ccgccttgac gatgccggca tcggtcttga
      541 aatatgtctt gaggtctcga acgtcgagga cgacctctct atcgttgatt ggactcatgc
      601 cgtcatgctc ctacagttca ttctttgatt gcgggtcgaa cgcgtcgcgt aggccgtctc
      661 cgaagaagtt cattgcgaac aaaaagaggg tcatcgcgat cgccgggaag atcaggcgcc
      721 acggatagag ggtcatcccg tcgaccgcat cgccgacgag cgagccccat gaagcgaacg
      781 gggcttggac tccgaggccc aggaaggaaa gaaacgactc ctgcatgatg aaggcgggga
      841 cgcggagggt ggaatacacg atgatcacgc tcagcgagtt gggaatcata tgcctgagga
      901 tgatccgggc ggtcgatccg cccatggacc gggcggcttc gacgtactcg ctgtttttca
      961 ggctcatcac ctggccgcga accatccggg cgatcgtcag ccaggagaca agggcgaggg
     1021 ctccgaagag gttgagaatg ctccgtccga agatcgccat gaagatgatg acgaggagca
     1081 tatacggaag gccgtacatg atgtcgacga tccgcatcag gatgtaatcg acctttccgc
     1141 cgacgtaccc ggcgatcgct ccgaggacga tgccgatcag cacgctggtg agcgtcccga
     1201 ccagcccgat cgcgatggac acctgactgc cgtagatgat ccggctgagc atgtcccgcc
     1261 cgctgtagtc ggttccgagg taatacatcc gctcatgctt cttcgtcgcc gtcaccttcg
     1321 cctgcaactg ctcgttgacg tcgatgatcc gttccacggg gaagaccgca agaccctctt
     1381 tgatctcttt cttcaaatca cggtccgcct gacgggtgat cgtggtggag atctttccga
     1441 ggaggttcgt ctttccaagc ttggcgatcc cgccctctcc gatcgtctga agctccagct
     1501 ccagattcac ttcatattcc tcggcgctga ggccgggggt ggtgctcttg agggtgttca
     1561 tcagctggct ggtcacttcc gtgagggttt gcgcttcgac ggcttcgatg ggcgtcttgg
     1621 tcaaattcgc atagattcgg gagatctcct catagctcat cttgttgaga tcctcccgtt
     1681 tgccgccctt ctcctcgctg aacgtgattt tggtgacgtt gatcaggaac tcggtatcga
     1741 tcttctcctg aaggcgggtg atgacccgct gatcggcggc attgaagacg aacgtgcccg
     1801 cttcccgctg ggcttccccc tctttgagga cgaagttcca aaccttgttt gtatcattgg
     1861 cggcgatcca cttcctgatc tgggcgctct gtgcttccgt tactacaagc tcgccgctcc
     1921 gccatgcctt gaaatagagg tctttgaggc ggttctccat catgatttcg ccggctttcc
     1981 gggtcagcga agggcgcaga tgctgatggt cgagaatgat ctggtcatag ggatggatcg
     2041 gaagaagggg agccgtggcg gcaaggatcg agtagaggat gacgataatc attccgacga
     2101 ccgccatctt gttttttttc aaccgtctcc aggcatcctt tccgaggctg ttgccctcga
     2161 ccacctggtt gaattcattc gttgttacta cgttcttcat ctcgatctcc tctacttgta
     2221 ggtgatccgc ggatcaagct gggcatagat gatgtcgacg atgaagttca tgacgatcaa
     2281 gatgaccgaa tagacgatga ctacgccgac gatgagggtg tagtcccggt tgaatgagga
     2341 ttgaacgaag aattttccca agcccggaac ccggaagatc tgttcgacga cgacgcttcc
     2401 ggtgatgatt ccggcgaatg cggggccgag atagctgacg acgggcagga gggcgccctt
     2461 catcgcgtgc ttgaagatga tcgtcgagcg cttcatccct tttgcttttg cggtccggat
     2521 gtagtcgctt cggagcgttt ccagcatcga cgagcgggtg aggcgggcga tgttggcgaa
     2581 gtattcaaag ctcagcgaga ccgccggaag gatgatcgtc agataccctt cgccggtggt
     2641 gtaccatccg ctggtcggaa gccatttgag ctttaccgcg aaaatgagct gcagaaccgg
     2701 agcgatgacg aagagcggga cgctgattcc gatgaccgcc aaccccatcg agagataatc
     2761 gatccaggag ttttgccgca ccgcggcgat gatgccgatt cccattccga tcgttacgct
     2821 gatgagcatc gcccacaacc ccagaacgat cgatttggga aggctggtga agatgtaata
     2881 gtttacatcg tagtccttgt acttgaagga ggggccaagg tctcctctca tgatgtcgaa
     2941 catatatcgt ccatattgct tgatcagcgg ttcgtcgagg tgatatttcg cctcgatgtt
     3001 ccgtcggacg acgtcggtga ggtttcgttc ggcggaaagg ggtcctccgg gagcgatccg
     3061 gactatgaag aagcttaggg tgatgatgat gaagagcgtg gggatcatcc ctaagagcct
     3121 gttaacaata tatttcgtca tgcaatgcct cgtattcgag aagaaccgtc ggagatcaat
     3181 gccctccgac aagcggtctt gttgatacta taccaagaag gatagtttgg caagaattgg
     3241 gaaaaccccg tttccccatg gctggacaat gctttctccc ttgggtagag ttctgaaacg
     3301 tgatgaagac cgtatatctg gaaaatctgg gatgctcgaa gaatcaggtg gatgccgaag
     3361 tgatgctcac tcttttacag gatgagttct cccgaacgga ggatccggcc gccgccgatt
     3421 tgatcatcgt caacacctgc ggcttcatcg aatccgcccg ggctgaggcg atcgactcct
     3481 ttttcgacct gtacgggagc aatcccgaag caaagatcat cttcagcggc tgcatggccc
     3541 agcggtacgc cgatgaactg tcggcggaat tgacggaggc gtcggcgatc ttcggcaacc
     3601 gcgatctttc aaagctgccc gaggtggtcg ggcaggtctt ttcaggcgag cgggtcgtct
     3661 ccactccgga gtatccggca aacagctttg aagatgtcta taaacgaagt cggttgctct
     3721 cattcgacgg aagcgcgtat gtgaagatca gcgaggggtg caaccatcgc tgctcctact
     3781 gcgccattcc catcatccgc ggttccctgc gttccaaacc gatggcgatc atcctcgaag
     3841 aggtcacctc cctcattgcc cggggagtca aggagatcaa tctcatcggc caggatctgg
     3901 cctcctgggg aagggatctg aaggaagcgg atgcgcattt catggctctg cttaaacgga
     3961 ttgccgaggt ggagggggat ttcgtcatcc gtctgctgta tatccacccc gacacgtttc
     4021 ccatggagct gttggagttc attcggaacc atccgaagat ccttccgtac ttcgacatcc
     4081 cgttccagca tgcaacggag agcatccttc ggtcgatggg aagaagggga acgaaggaat
     4141 cccaccttgc gttgactcgg aagatcaggg aaatgttgcc cgatgcagtg atccgctcga
     4201 cgatcctgct cggctatccg ggcgaggatg aggattcgtt tgccgaggtg ctggatttcc
     4261 tcattcaggc gcagctcgat tgggtcggct cgttcgttta ttcgctggag gagggcacga
     4321 aagccgccga attgacgaat gagcgagccc acaggaaact cgcttccaaa gcgcgaaggt
     4381 ttcaacagga gcttgaagcg cttcagctct ccatcactca aagacgcctt gaaacctttg
     4441 tcggcaggga ggtcgacgtg ctcattgaag agcatgtcga gggcgaggag ctctacatcg
     4501 gccgaaccta cgcccaggcg cccgaggtgg acggcctgac cgtggtgatg ggccgagatt
     4561 tggtaccggg aaccgtggtt cggtgcggga taacccgggt gaacgggatc gacttcgagg
     4621 cggttcccct ccatggagga tatgatggct cccattgaaa catacttgaa gcaactgaac
     4681 gaaccccaac gggaggcggt gcttgaaaac agccggccgc tcttgatgct ggccggggcc
     4741 ggaagcggca agacccgggt gatcaccacc aagatcgcct atgcgatcga ggagttgaag
     4801 attcctcctt ataagattct cgccgtcacg ttcaccaaca aggctgcaaa cgagatgaaa
     4861 gagcgggtcg aagcgatgct tccggataat ccggacgtga aggagtgcaa tatccggacg
     4921 ttccactcct tcggcgcatg gctcttaagg agattcggaa gcgagatcgg tctggagaat
     4981 aatttcacga tctatgatga cgacgattcg ctttcgctgc ttgcttcaat ctatccttcc
     5041 tacaagaagc gggagcttga accgatcatg aagcggatca gccacgccaa ggatcggatg
     5101 ctctcccccg gtgacgcgat gaagcgcttt cacgccgatc tgctcttcaa gcagatgtat
     5161 gagcgatatg aagaaaagct tcgagcggtg ggaaaccttg atttcgccga tctgatcggt
     5221 cgctccatcg agctgctccg ggatgccccc catgtgcagg agtggatcca ctcccgtttt
     5281 gcgatgatcc tggtcgacga atatcaggac tccaacaccg cccagttcct tctgctgaag
     5341 ctccttgtcg gtcccgagac cttcgtcggg gtggtcgggg acgacgacca gtcgatctac
     5401 cgcttccggg gggcggaagt gaagaacatt cttaccttcg cttcctcgtt tccaaaagca
     5461 agaacgatca aactggagca gaactaccgt tcgaccactc agattttgga tgtggccaac
     5521 agcatcatct ccaagaatgc cgatcggcac gataagaacc tttggagcgc caggaagggt
     5581 ggacggaagc ccaagctctt ctacgtgcac gatgaagagg atgaggcccg caggatcgtc
     5641 acgcttcttc gggaggatcg caactacaac agcagcgcgg tgctctatcg aaccaacgcc
     5701 cagagcgtcc ccttcgaaac ggtgttcaaa cggtatggga ttccttataa agtggtggga
     5761 gctctgcaat tctacgaacg ggaagaggtg aaggatgctc tggcgctcct gtacctgctg
     5821 accaacccga aggacgaagt ctccttccga aggatcatca acaagcccgc tcgggggatc
     5881 gggccgggca gtgtcggcaa gatcatcggc ttcgctccgc agacgggcgg cgatcttctg
     5941 caggctgccg catcggcggt gcaaaaccgg acgctctccc cgaaagccgc ccagggagcc
     6001 gagctcgcgg ttcgcctcat cgatgaaggc cgaaagctca ttgtcgatgg aaatctggtg
     6061 gacggggtga cgttcctgat caacgagtcg ggcctctatg agtattaccg ggagctggat
     6121 ctcctcaaca ccacccagaa agtcgccaac ctcgaagcgc tggtaaacgc catttcggtg
     6181 tatgagccct cgtttgatgg gcttgtcgcg tttctggagc agctgaccct cgatcgaacg
     6241 acgttgggaa cggacgatcc ccgggacagg gacggggtga ccctcatcac gatgcacaac
     6301 accaagggcc tggagttcga ccgggtgttc gtcaccggct tggaggaggg attgtttccc
     6361 ggccggaaaa atgagaggga ggaggatatc caagaggagc ggcggatctt ctacgtcgcg
     6421 gtcacccggg ctcggaacga actctatctc ttctcctcga aggttcggaa gatctgggga
     6481 aagaccagct tccagaatcc gagccgcttc atcgacgata tcgatccctc cctgattcag
     6541 gttgaagggt ctcctgcgcc gcagggattc gactcgtttg cgaagcggga tgccgtttca
     6601 accgattggg gaagcagtgg ttcgggcatg atccgtcagg gattcgcccc cggggcaaaa
     6661 cggttcgaga tgaaggtgaa gcatgagaag cagggagaca gtcccctgtt ccccgtcggc
     6721 cagaaggtgt acagcgatgc aaatggggaa ggggaagtgg tcaagattcg gggtgaaggg
     6781 gatcgagagg cgattgatgt cgtcttttcc accgggaaga aggccaccta cctgagccgc
     6841 tttgccgacc ttgatcgcat tggcggtgaa taaggctaga cacgattggg cggaagatta
     6901 tacaatgctt accgttgtcc gcccttcggg gcgtggattt tttcatggac atggcgttgc
     6961 gccgtgaaga acccgatcat ggtcgatccg tttcaagcac cctttggatc ttgtcggagc
     7021 acgatggtgc gctccttcct aacctgcctt tcccggggag ttatccaaga ccgaagcctt
     7081 gaccggatgt aatgatcggc acgcggagag atcgttgcta cgttgctcag tagccaatcg
     7141 atttcttctc ttccggagaa gccgtcgatg caggactctg catcatgatt acaggcaagg
     7201 agtgaccttg cagagcagga tgaatggatg aagactattt ttgtgaaacc gctgacgatt
     7261 cagaagaagt ggtatctgat tgatgcagag ggaaaggagc ttggccgagt ggcggtcgcc
     7321 gcagcgcgca tcctccgcgg gaagaacaaa gccgagtacg cccccctcca cgaccttggt
     7381 gactatgtga tcatcatcaa cgctgaaaaa gcgatcctta ccggaaataa gtacaccgac
     7441 aagatgtact acagccactc aggcttcccc gggggactgc gtaagaccag ctatgccgac
     7501 atgatcaagc gcaagccgac gttcccgatg gaacacgccg tcaaagggat gctgcccaag
     7561 ggtccgcttg gccgcaagct gttcaccaac atgcgggtct atgccggtgc cgagcataag
     7621 caaagcgccc agcagccgat tccggtcgaa atctaaggta ggagcgaata atggcaaaga
     7681 aagaagctac taaggcagtg aaccttggtc atggtgttgg ccgccgcaag accgcggtcg
     7741 ctcgggtcta tcttcgtgaa ggcgaaggtg cgattatgat caacggccgg gatgtcgaca
     7801 gctatttcgg caacccgatg cttacgttca tcgtcaagca accgttggca gccaccgaga
     7861 cggatggcaa gtacgacatc ctcatcaacg tgaagggcgg aggagtctcc ggccaggccg
     7921 gggcatgccg ccatggaatc gcccgcgccc tcgccattca tgacgaagct ttcaggccgg
     7981 ttcttcgagc cgccgggttc atgacccgcg actcgcgaat ggctgagcgg aagaagtacg
     8041 gtcagcctgg cgcccgccga aagttccagt tctccaagcg ctaaggtgtc ttacgcacgg
     8101 atcgtcaggg agcccggaaa gggagggtat cagctcctcc cgagccaagg ctcccctttt
     8161 tatatctccc cggacgtgtt ccgcaccctt ggcttgaggg ttgatcagga ggtcgatgaa
     8221 gagcttgccg ccaagctctt tcgggaaagt caaaaacagg ggtgctacgc ccaaggtctt
     8281 tcctacctcg cccgaagcga gcataccacg tttcacctcg ttcagaagct gacccggaaa
     8341 ggatacccga aagagatcat tgatgatgcc gtttcccgac tcaccgagga gggggcgctc
     8401 agcgatctgc gctacgcccg gctgttcatc gaacgcaggc agcgcaagaa tcccgaaggc
     8461 cgcttgcgtc tggctcagcg tcttgccgat cggggagtca ggaacgatgc cgccctgcag
     8521 gcattggatg aactcttcac cgaagagcat attgccacct atgtagcccg ggcactgaac
     8581 cgggcgaaga ccggctatcc ggagaaaaat gtccgggatc ttcttcgctt gtgggggttt
     8641 tcctcctatg agatccgaac ggggcttgaa ctggccggcg atgatgaatt accgtaaagc
     8701 cgtgaccaaa aaaattctcg ttctctttgc aatcatagct gttttctcaa ccggactctc
     8761 cgcgttccgc ttcgactatt ccgaagacct cgaccggatc ggtgatgtgg ggatgtacct
     8821 gagcctggcg cttccgggaa cccttctctt tg
//
LOCUS       DAOSLI010000063         8768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_7529_length_8768_cov_23.019626, whole genome shotgun sequence.
ACCESSION   DAOSLI010000063 DAOSLI010000000
VERSION     DAOSLI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7529_length_8768_cov_23.019626"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLF67_07940"
     CDS             <1..273
                     /locus_tag="PLF67_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE89977.1"
                     /translation="GALFGTKGYIEFENINNCEGIQVYDKEYNLIETYKPFKQITGFE
                     FQVEACKRAIEAGKTECEQMPHSEILAVMNVMDELRRQWNLVYPME"
     gene            276..770
                     /locus_tag="PLF67_07945"
     CDS             276..770
                     /locus_tag="PLF67_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOE89978.1"
                     /translation="MELQELIALVDRQEELLRLKTFNSSIAWKLGNLFHEKIVKEDLP
                     IAVSIRSINGKTLFHYSSDKANYGSQGWLDRKFNTVRHFETSTLRYALFLRSRGATLL
                     ERGLDPSKFVSGGGGFPIRTIDSTLVGAVLVSGMKDTEDHAIIIEVLSQYLDVINVPV
                     YPIS"
     gene            complement(755..1303)
                     /gene="cyaB"
                     /locus_tag="PLF67_07950"
     CDS             complement(755..1303)
                     /gene="cyaB"
                     /locus_tag="PLF67_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOE89979.1"
                     /translation="MAFEVELKAWVRKAEALKRSLDTKEGLLYVGFEEKEDAYYALEE
                     LPLLRIRKEVVNEERSLVVTHKKRSTIDGIEENLEYEFSVDPLQEQAVHAFFTALGYR
                     VAKRKFKRGWRYRQVHTTTTPGLTVELVHLEGLGWFIEVEALVNTREERRQAREELMA
                     FLASLGIDSSAIESKPYLQLIG"
     gene            complement(1305..2081)
                     /locus_tag="PLF67_07955"
     CDS             complement(1305..2081)
                     /locus_tag="PLF67_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89980.1"
                     /translation="MVGFFIKKAFFDGWDNLISMVVHNLGYLLLLFAFFGVLSLVSTS
                     TLLFFIVIILGTGALAFYTAGASSATWAYARYQRPGWSDFKRGIRISWRHALLFWFLL
                     LLVLTLVFLVIPFYLSFENIVGIILGVIIFWVTLLLIFALMYFFPLFFAMQGDRPMKT
                     LKKSFLLVLDNPFFTLFLAVYQVINTLFSLLLAFLAPGFTGISLAGSVATKLLLFKYD
                     YLEEHPETGRKAIPWDDLLYEEREAVGPRSLKGMIFPWKD"
     gene            complement(2081..2917)
                     /locus_tag="PLF67_07960"
     CDS             complement(2081..2917)
                     /locus_tag="PLF67_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE89981.1"
                     /translation="MEYKTFSNGVKIPVVGFGTWQVSPDEAYESVRSALEVGYRHIDT
                     AAAYENEAEVGQAITDSGIRREEIFVTTKLWNAVRGYKETLQAAKESMEKLQVEYLDL
                     YLIHWPNPAAHRHWWKEANAESWKAMEELYENGAIKAIGISNFEERHIEALLASAQVI
                     PHTNQIRLFAGEQQRTLVAYCRNKGMITEAYSPLGVGGILSNEVIGRIAKETKRTPAQ
                     VALRYNLQKDHVVLPKSVHRDRMVENLNLFDFELSREQMISLDALPNTISATRNPDEV
                     PF"
     gene            3022..3094
                     /locus_tag="PLF67_07965"
     tRNA            3022..3094
                     /locus_tag="PLF67_07965"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3055..3057,aa:Met,seq:cat)
     gene            complement(3134..4492)
                     /locus_tag="PLF67_07970"
     CDS             complement(3134..4492)
                     /locus_tag="PLF67_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOE89982.1"
                     /translation="MHIVLIGAGSAQFGLGSLGDIMQSKLLAGSSIALVDIDEQALSR
                     VNAIAQAFIKEKGLNFTVTATTDRKAALKGADAVIISIEVGQRFPLWEEDWTLPQQYG
                     SQQIYGENGGPGGLFHALRITPVIVEMCDDVMEVCPDAWVFNYSNPMTAITTTVLRKY
                     PDLKFVGLCHEIASLERYLPSILNTPFENLELKAAGLNHFSVLLSARYKDSGKDAYPD
                     ILEKAPAFFEREPGYSDILAYLKAHPQELQTEGSTNRALPPEIRSRKSWADRGLFREI
                     LETYKLLPITVDSHIGEYISWAHEVADHKGIKDFFELYQIMLGFKKTRIELKAHERIS
                     FILDGIVSNSGYEESAVNIRNDHLIPDLPSWIAVEVPARVYAKGMEGIAFPDYPKGFG
                     ALLRNYTGVYDLTAEAILRKKKEYAIQALLASPITTPVRAASELVELMIQRQEKWLGY
                     LK"
     gene            4563..5087
                     /gene="thpR"
                     /locus_tag="PLF67_07975"
     CDS             4563..5087
                     /gene="thpR"
                     /locus_tag="PLF67_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOE89983.1"
                     /translation="MRLFFGLTFSEEDKITLARLSGSIDPYITKGKAVREENYHLTLS
                     FIGEVEQKYLDTCCSILDRIDPPVLTLNMKRLGLFPSRRHNTLILHVEPDERLTVLQQ
                     TLEQYLVSKKLIAKGGKYKPHITLFRQAVIPIVPMIEPIEISPTSVALFHSHRENNLL
                     TYTVVCEKELGIDE"
     gene            5080..5874
                     /locus_tag="PLF67_07980"
     CDS             5080..5874
                     /locus_tag="PLF67_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE89984.1"
                     /translation="MNESRYIRYNERAWDREVDRKSIWTDGLTEEELQKALSGTVEIR
                     LTTYKPMPSDWLGDLAGKRVLAVGSGGGQQAILFALAGSEVTLLDLSGKQLEQDRTMA
                     NRLGLELTLIKGDMRDLSQFEGEAFDIVFNPASTVFIDDVRSFYRGCARVLKPKGVFL
                     TSITNPVLYLFDERLALKNKLKVKYTLPYSDIRSLSRKRIEKMIKTGATFEFSHTLAD
                     LLGGLGDAGFVIEGVYSDRCGFELLDSFIADAYLAVRATKRLPHDS"
     gene            5893..6717
                     /locus_tag="PLF67_07985"
     CDS             5893..6717
                     /locus_tag="PLF67_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89985.1"
                     /translation="MENYLKKVLITKGLDDPIAFAKAQQRIAELEEKRIDSFNEFGPL
                     HERYLVINDLLLGAYENAGDTLNQIEAAETSYQALVHLLGRENQESSKAYIALGRAYL
                     QDGQIREAQKVCDYVLKAAIGERDDYSWELYVDTLSLHADILFVKGNHHEELRYRKHL
                     YDIANQHFGIADERTVLLRVALAYCLEKLRRFKEASEHYVVVRAYMEQDRTFATDAEK
                     IGLTVHLARCYYMIGQKDDARQIYGWAYSLARRTYGPSSALTKKTLRILKHINRLG"
     gene            6860..7474
                     /locus_tag="PLF67_07990"
     CDS             6860..7474
                     /locus_tag="PLF67_07990"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOE89986.1"
                     /translation="MFVKYTLPYSYDAMEPHIDALTMETHYTKHHEGYTNNLNDAVAK
                     AQVDKDIASLLGSLDEIADPALRRTIRNNGGGYYNHNLYFATISPDGGKDPSGAFGNL
                     LKSEFGSIDELKAKLSAFALGQFGSGWAWLSANKDGKLLLSSTPNQDNPLMDGKGYTP
                     ILGIDVWEHAYYLKYKNLRGEYVKAFFNVVDWDEVAGNYEKIRK"
     gene            7605..8609
                     /locus_tag="PLF67_07995"
     CDS             7605..8609
                     /locus_tag="PLF67_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOE89987.1"
                     /translation="MIEERIIQNPKNGLVVLIVNTLAILIAIGVFVASIVMGRATYPG
                     LLSIGLTVVSALYAFIIGPIMYVGLKVLTKNEALVLTLFGKYYGTLKQDGFFFVNPFC
                     SAFNPTAGTNPSTGATREKKKEQIVVSPQGMNVEFKLSKKKISLKAMTLNNDKQKIND
                     SLGNPIIIGVVVIWKVVDTAKAVFNVDNYIEYISIQTDASLRNAVRLYPYDSEDDEKS
                     LRGSANEVADDVKKQLQEKVEIAGLEILETRITHLSYAPEIAAAMLQRQQATAIIAAR
                     KKIVEGAVGMVEMALEQLSAKGVVDLDEERKASMVSNLMVVLSSNRDTQPVVNTGSIY
                     "
BASE COUNT         2110 a         2375 c         2308 g         1975 t
ORIGIN      
        1 ggagcgctgt tcggcacaaa aggatacatc gaattcgaaa acatcaacaa ctgtgaagga
       61 atccaagtct acgacaaaga gtacaatctg attgaaacct acaaaccctt caagcagatc
      121 accggtttcg agttccaagt cgaagcgtgc aaacgggcga tcgaagcggg aaaaaccgaa
      181 tgcgagcaga tgccgcacag tgagatcctt gccgtgatga acgtgatgga tgagctcaga
      241 cgccaatgga acttggtgta tccgatggag tgatgatgga actgcaagaa ttaattgctc
      301 ttgtcgatcg acaggaagag ctgctccggt tgaaaacgtt caacagttcg attgcgtgga
      361 aactcgggaa tttgtttcat gagaagatcg tgaaagaaga tcttccaatc gccgtgtcga
      421 ttcgctcgat caacggaaaa acccttttcc actacagcag tgataaggcg aattatggct
      481 cccagggatg gttggaccga aagttcaata cggtccggca cttcgaaaca tccaccctgc
      541 ggtacgccct ttttctccga agccgaggcg ccaccctgct cgaacgggga ttggatccgt
      601 caaagttcgt ttccggtggc ggcggatttc cgattcgcac cattgattcg acgttggtcg
      661 gggccgttct ggtctccggt atgaaggaca cggaggatca tgcgatcatc attgaggtcc
      721 tcagtcagta tctggatgtc ataaacgtcc ctgtctaccc gatcagctga aggtagggct
      781 tggactcgat cgccgaggaa tcgattccca gtgaggcaag aaacgccatc agctcctctc
      841 tggcctgccg gcgttcctcc ctcgtgttca ccagcgcttc aacctcgatg aaccagccga
      901 gcccttcaag gtggacgagt tccaccgtca atccgggggt ggtggtcgtg tggacttgcc
      961 ggtagcgcca tccccgtttg aattttctct tcgctacgcg atagccgagc gcggtaaaga
     1021 aggcgtgaac cgcctgttcc tgcaggggat cgacggaaaa ttcatattca agattctctt
     1081 cgattccatc gatggtggag cgcttcttgt gagtgaccac caaagaccgc tcctcattga
     1141 ccacctcttt tcgaatccga aggagcggga gctcttccaa ggcataatat gcatcctctt
     1201 tctcttcgaa gccgacatag agaaggccct ctttcgtatc gaggctcctc ttcaacgctt
     1261 cggccttgcg aacccacgcc ttgagctcca cttcaaaagc catctcaatc cttccacggg
     1321 aagatcattc ccttcagcga tctgggaccg accgcttccc gttcctcata gagcagatca
     1381 tcccagggaa tcgctttgcg accggtttcg gggtgctctt ccaaataatc gtatttgaag
     1441 aggagcagct tcgtggcaac gctgccggca aggctgatgc cggtgaaccc gggagccaaa
     1501 aaagccaaca gaagcgaaaa gagggtgttt atcacttggt acactgccag aaagagggta
     1561 aagaaggggt tgtccaggac aagcaggaag gattttttaa gcgtcttcat cggacggtcg
     1621 ccctgcatcg caaagaacaa ggggaagaag tacatcagag cgaaaatgag cagcagcgtc
     1681 acccaaaaga tgatgacccc caggatgatt cccacaatgt tctcgaagct caggtaaaac
     1741 gggatcacca gaaagaccag cgtgagaaca agcagcagca gaaaccaaaa gagcagagcg
     1801 tgacgccatg agatccgaat cccccgctta aaatcgctcc accccggtcg ttggtagcgg
     1861 gcgtacgccc aggtggcgga agaagctccg gcggtataga acgcaagcgc tcccgtgccg
     1921 aggatgatga cgataaagaa cagcagagtg gatgtcgaaa cgagtgaaag cacgccaaag
     1981 aacgcgaaga gaagcagaag gtaacccaag ttatggacta ccatcgaaat cagattgtcc
     2041 catccgtcga agaacgcctt ttttatgaaa aacccaacca ttagaaggga acctcgtccg
     2101 ggttgcgcgt tgcgctgatc gtattcggaa gggcatccag gctgatcatc tgctcccggc
     2161 tcagctcgaa atcgaagagg ttgaggtttt caaccatccg gtcgcggtgt acgctctttg
     2221 gaagcacgac gtgatccttt tgaagattgt agcgcagcgc cacttgagcg ggcgtgcgct
     2281 tcgtctcctt cgctattcgt ccgatcacct cgttggagag gattccacct accccgaggg
     2341 gactgtatgc ttcggtgatc atccctttat ttcgacagta ggcgaccaat gtgcgctgct
     2401 gttctccggc aaagagccgg atctggttcg tatggggaat aacctgagcg ctcgcaagaa
     2461 gcgcttcgat atggcgctcc tcgaagttac tgatcccgat cgctttgatc gctccgtttt
     2521 catagagctc ctccatcgcc ttccagcttt cggcgttggc ctctttccac cagtgtcggt
     2581 gggcggcggg attgggccag tggatgaggt acaggtcgag gtattcaacc tggagttttt
     2641 ccatcgactc ctttgccgct tgaagcgttt ctttataccc tcgaacagca ttccacagct
     2701 tggtggtaac aaagatctcc tcccgcctga tgccgctgtc ggtgatcgcc tgaccgactt
     2761 cagcttcatt ttcatatgct gcggcggtat caatgtgccg gtatcccacc tccaatgcgg
     2821 aacggacgct ttcatatgcc tcatccggag agacttgcca cgttccaaac ccgacgacgg
     2881 gaattttgac cccgttgctg aacgttttat attccatttc gttcctcact tcttcaaagt
     2941 cctatcctac cacaaaggca aatcatcctc aaaccccctt gacggagcta aagaggtttg
     3001 gctatagtaa gctcgttgtt tggcggcata gctcagttgg tagagcaagc ggctcatatc
     3061 cgctaggtca ggggtccgag tccctttgcc gctaaaatgg cctccccgca tgttgaggag
     3121 gcctcttttt ttcttatttc aggtacccca accacttttc ctgacgttga atcatcagct
     3181 ccacgagctc gctcgcagct ctgacgggtg ttgtaatcgg actggcgagg agtgcctgga
     3241 tcgcatactc cttcttcttc ctgaggatcg cttcggcagt caggtcatac actcccgtgt
     3301 agtttctaag cagggctccg aaccccttcg gataatcggg gaacgcgatt ccctccatcc
     3361 ctttggcata gacgcgggcg ggcacctcga cggcgatcca ggaaggaaga tccggaatca
     3421 ggtggtcgtt tcggatgttc accgcactct cctcataccc ggagttggag acaatcccgt
     3481 ccaggatgaa ggagatccgc tcgtgggcct tgagctcgat tctggtcttt ttgaaaccga
     3541 gcataatctg gtagagctcg aagaagtcct tgatgccctt gtgatcggcg acttcatggg
     3601 cccaggagat atattcaccg atgtgcgaat cgacggtgat cggaagaagc ttgtaggttt
     3661 caaggatctc cctgaacaag ccccggtccg cccagctctt cctgcttcgg atctccggag
     3721 gaagagcccg gttggtcgag ccttcggtct gcagctcctg cggatgtgct ttgaggtatg
     3781 caagaatatc gctgtagccc ggttcgcgct cgaagaacgc gggcgctttt tcaaggatat
     3841 cgggataggc gtccttgccg ctgtccttgt agcgggcgct gaggaggaca ctgaagtggt
     3901 tcaaccccgc cgctttgagc tcgaggttct caaacggcgt attgagaatg gagggaagat
     3961 aacgctcaag cgaggcgatt tcatggcaga gccccacgaa cttcagatcc gggtatttcc
     4021 tgaggacggt ggtggtgatc gccgtcatcg ggttcgagta gttgaacacc cacgcatcgg
     4081 gacagacttc catcacgtcg tcacacatct caacgatgac cggggtgatc ctcagggcgt
     4141 ggaagagtcc gccggggccg ccgttctcac catagatctg ctgtgatccg tactgctggg
     4201 gaagggtcca atcctcctcc cagaggggga agcgctggcc gacttcgatc gagataatca
     4261 ccgcatcagc gcctttgagg gcggcttttc ggtcggtggt cgccgttacg gtgaagttca
     4321 atcccttttc tttgatgaac gcctgggcga tcgcattcac tcgcgaaagc gcttgttcgt
     4381 cgatatcaac caatgcgatc gaagagcctg cgagcagctt gctctgcatg atgtccccca
     4441 aggaccctaa accgaactgg gcgcttccgg ccccgattaa aacaatgtgc atatgagcct
     4501 ccttccccac tatacaccaa cgagaatgtt ttattccatc gatcatcgtg tatactctag
     4561 gtatgcgtct ttttttcggt ctgacttttt cagaagaaga caagatcacc ctcgcccgtc
     4621 taagcggatc gatcgatccg tatatcacca aaggaaaggc ggttcgcgag gagaactacc
     4681 acctcaccct ctccttcatc ggcgaagtcg aacaaaaata tctggatacc tgttgttcga
     4741 tccttgatcg gattgatccg cccgtcctca cgctgaacat gaaacggctc gggctcttcc
     4801 cttccagaag acacaacacc cttattcttc acgtcgaacc cgatgaacgg cttaccgtcc
     4861 tgcagcagac gcttgaacag tacctcgtct ccaaaaaact catcgccaaa ggggggaaat
     4921 ataagccgca catcaccctt tttcgccaag ccgtcattcc gatcgtgccg atgatcgaac
     4981 cgatcgaaat ctctccgacc tcggttgctt tgtttcactc ccatcgggaa aacaatctct
     5041 tgacgtatac cgttgtctgt gaaaaggagc ttggaatcga tgaatgagag ccgatacatc
     5101 cgctacaacg aacgagcatg ggatcgcgag gtcgatagaa agagcatctg gacggatggc
     5161 ctgaccgagg aggaattgca aaaagcgtta tcggggacgg tcgaaatccg gcttaccacg
     5221 tacaagccga tgccttccga ctggcttgga gacctcgcgg gcaaacgggt gctcgcagtc
     5281 ggatccggtg gcgggcagca ggcgattctg ttcgccttgg ccggcagcga ggtcacgctt
     5341 cttgatctct ccggcaaaca gctcgagcag gaccgaacga tggccaatcg attgggcctc
     5401 gagctgacct tgatcaaggg tgatatgagg gatctctctc aatttgaggg ggaagccttt
     5461 gacatcgtct tcaatcccgc ttccacggtg ttcattgatg acgtccgctc cttctaccgg
     5521 ggatgtgctc gcgtgctcaa acctaaagga gtctttttga ccagcatcac caatcccgtc
     5581 ctctatctct tcgatgaacg actggccctc aaaaacaagc tgaaagtcaa atacaccctt
     5641 ccctacagcg atatccggag tttgagcagg aaacggatcg agaagatgat caagaccggt
     5701 gccaccttcg agttcagcca caccctcgcc gatctgctcg gcggccttgg ggatgcgggg
     5761 ttcgtcatcg aaggggtgta tagtgatcga tgcggcttcg aactgcttga ttccttcatc
     5821 gccgatgctt acttggcggt ccgggctacg aaacgcttgc cccatgactc gtagcttggg
     5881 tattatacag gtatggagaa ctatctgaag aaagtcctga tcaccaaagg cctcgatgac
     5941 ccgatcgcct tcgccaaggc tcagcagcgg attgccgagc tggaggagaa gcggatcgat
     6001 tcattcaatg agttcggccc gctccatgag cggtatctgg tgatcaacga cctgctcctc
     6061 ggggcgtacg agaacgccgg tgatacgctc aatcagattg aagccgccga aacctcctac
     6121 caagccctcg tccacctgct tggcagggag aaccaggaaa gttcaaaagc atatatcgcc
     6181 ctcggccggg cgtatctgca ggatgggcag atcagggaag ctcagaaagt gtgtgattat
     6241 gttctcaagg cggcgatcgg tgaacgggat gattactctt gggagctcta tgtcgatacc
     6301 ctcagcctcc atgccgacat cctctttgtc aaaggcaatc accacgaaga gctgcgctat
     6361 cgcaagcatc tttacgacat cgccaatcaa cacttcggaa tcgccgatga acggaccgtg
     6421 ctcttgcggg tagccctcgc ctactgcttg gaaaagctcc gccgcttcaa ggaagcaagc
     6481 gaacactacg tcgtcgtcag agcctacatg gagcaggatc ggacgttcgc aaccgatgcg
     6541 gagaagatcg gtctgaccgt ccatctggcc cggtgctact acatgatcgg acaaaaggac
     6601 gatgcaagac agatctacgg ctgggcgtac tccctcgccc ggcgaaccta cggtccctcc
     6661 tccgctctta ccaagaaaac gcttcgcatt ctcaaacaca tcaatcgact cggctgagag
     6721 tctcctgttc ccttttgcac caaacaatga aaattcaatg gatgacggtt tgaacccttg
     6781 cctcacaggc gttccttgag tatctttagc taaagttagc aaaaggcaac ttatcataac
     6841 tctttatgga ggtatcacca tgttcgtaaa atatacattg ccctattcct atgatgcgat
     6901 ggagccgcac attgatgcgc tcacgatgga gacccactac accaagcacc atgaaggata
     6961 taccaacaac ctgaacgatg cggtcgcaaa agcccaagtg gacaaggaca ttgcatccct
     7021 gctgggctcg ttggatgaga tcgccgatcc cgccttgcgt cggacgatcc gaaacaacgg
     7081 cggcggatac tataaccaca acctctactt cgcaacgatc tctccggacg ggggaaaaga
     7141 tccctcaggg gcgttcggga atctgctgaa gagcgaattc ggaagtatcg atgaactgaa
     7201 agccaaactg agcgcgttcg ctctcggtca gttcggttcc ggctgggcgt ggctcagtgc
     7261 aaacaaggat gggaagctcc tcctctcctc gacccccaac caggacaacc ccctgatgga
     7321 cggaaagggc tacaccccga ttctcggtat cgatgtctgg gaacatgcct actacctcaa
     7381 gtacaaaaac ctccggggcg agtacgtgaa ggcgttcttc aacgtcgtcg attgggatga
     7441 ggtggccgga aattacgaga agatcagaaa ataagcgctt ctcatcagca caatgacggt
     7501 acagtcgatc cggctgtact gtttttttgt tttcgggggt tctttcatct tgtatatatt
     7561 ttatcattgt gatatcgtta tgatatcaga taaggagact ctgcatgata gaagagcgaa
     7621 tcattcaaaa cccgaaaaac ggactggtcg tcctcatcgt gaacacctta gcgattctca
     7681 tcgccatcgg ggtgttcgtc gcatcgatcg ttatggggcg agccacatat cccggcttgc
     7741 tttcgattgg tttgacggtc gtcagcgcac tctatgcatt catcatcggc ccgatcatgt
     7801 atgtagggtt gaaagtgctg acgaagaacg aggcgctcgt ccttaccctc ttcgggaagt
     7861 attacggaac cctcaagcag gatgggttct tcttcgtcaa tccgttctgc tccgccttca
     7921 atcccacggc cggaaccaat ccttccacgg gagcgacgag ggagaagaaa aaagaacaga
     7981 ttgtggtgtc ccctcaggga atgaacgttg aattcaagct gtcgaagaaa aagatcagtc
     8041 tcaaggcgat gacgctgaac aacgacaagc agaagatcaa cgacagtctg gggaacccga
     8101 tcatcatcgg cgttgtggtg atctggaagg tggtcgacac cgcaaaagcg gtcttcaacg
     8161 tcgacaacta cattgaatat atttcaattc aaaccgatgc ttcgctccga aatgcggtaa
     8221 gactctatcc ctacgacagc gaagatgacg aaaaatccct tcgagggtcg gcaaacgaag
     8281 ttgccgatga tgtgaagaaa caacttcagg aaaaagtcga aatagccgga ttggagattc
     8341 tggaaacgcg catcacccac ctctcctacg ccccggagat cgccgctgcg atgctccagc
     8401 gtcagcaggc cacggcgatc atcgccgccc ggaaaaagat tgtcgaaggt gcggtcggca
     8461 tggtcgagat ggcgttggag cagctcagcg ccaaaggcgt cgtagacctt gatgaagagc
     8521 gaaaggcttc gatggtcagc aacctgatgg tcgtcctttc ctccaatcgg gacacccagc
     8581 cggtggtcaa tacgggttcg atttactagt gatgaaagaa caggaacgga aacaggtatt
     8641 actccgactc agcggcgagc tttggaaaga gctcgcccgt tgggctgaag atgagttcag
     8701 atcaatcaac ggtcagatcg aatacctcct caccgatgcg gttcgaacca agcggaagaa
     8761 aacccttg
//
LOCUS       DAOSLI010000064         7646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_8827_length_7646_cov_23.938348, whole genome shotgun sequence.
ACCESSION   DAOSLI010000064 DAOSLI010000000
VERSION     DAOSLI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8827_length_7646_cov_23.938348"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1161
                     /locus_tag="PLF67_08000"
     CDS             130..1161
                     /locus_tag="PLF67_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOE89988.1"
                     /translation="MAIIPFYKPTLRRKDMDAVLQTMVDERIGPGERKNLFLKEFCDR
                     IGFAHGVALRTVIDAMICGLKILNLPEGSRIGTGVLAPSYYEDVVRSLGYELLLGDID
                     KEHGCLSQAEADRLVQEGCKALLLHEPVCQIPFGVDYKELGVPVMEDISQSIGSSFEE
                     RNAGSWGDLVVCSFEEDGVVSCGGGAALVFNEKEHKTAATELLSNTGSHVELPDMNAA
                     LGLIQLAALDEHLHRRRELYSLYTKSLMKTHHRLFGIGHIDFEPNGYGFCTTLDSKAE
                     DVIAFAKKYEVSAKRTFADSLASHHQEAFETYPVALPAFHRSISLPIYPFLSQSDLQV
                     ILKVIAHLP"
     gene            1172..2035
                     /locus_tag="PLF67_08005"
     CDS             1172..2035
                     /locus_tag="PLF67_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOE89989.1"
                     /translation="MSSKQVQKVLIVANWGKEEAHSLSATIKSYIEEKGIECTIWRTT
                     VEQDKPIVAEDTDLVICLGGDGTVLYCARHLQTLGIPILAINLGTFGYITEISVNEWK
                     EAVDRFLEGSYTLSRRLMIRVGVYRKGERVFTAHGLNEIVVSSSGISKVIGMSLKVGE
                     TPAGYFRADGMIIATPTGSTAYSLAAGGPILDAELSTLLITPICPFTLSNRPLVLSGD
                     SLITLTIAPNQRTGIVLSVDGQQNFPLQEGDELIVEKSRSRTLLVTSEKRNYIEVLRE
                     KLNWAGGAAHA"
     gene            2028..3695
                     /gene="recN"
                     /locus_tag="PLF67_08010"
     CDS             2028..3695
                     /gene="recN"
                     /locus_tag="PLF67_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOE89990.1"
                     /translation="MLERLQIHNYALIEDRQIEFEEGFTVITGETGAGKSIILGALSM
                     LMGDKVESHVIRSGSESATVNASFSFEEGLDEELRRFLSERDIDLGDDELLLSRTIRS
                     NGRSSALIQGRMVPRTDLGELAAQLFEISAQRDHQSLLKSEAQRNALDRSGECQGERA
                     SYQEEWSRLQLLKKELAERRALFEAAMREEEFLRFAEEELSAIDPKEGEDELLKGEIH
                     RLSSFEQIYESLTRSSSLLHSFEASSVLAALREAKHELEPAIKADRSLEDFANRLEES
                     AIEVQDIYESLRDHLSSLTFSEEELDRLQGRLAQIQRLKKKYGPSLERVLEFREEIAE
                     KLSMREDGEIHLSRLEKKIAEQEHLLLEAGKALRTKRKTAATRLAKNVESRLRTLGMK
                     EAVFEIRFREIPPAPHGLDEISFMICANPGLDLHPIDEVASGGELSRIMLALKVTLAR
                     QGELPTLIFDEVDAGIGGAVAVSVAKELAELGKTHQVIVITHLASIAGKADVHLTVSK
                     VVEAGMSHSRIKRVEGEERVREIARMLSGDSAREESLTHARLLLGDR"
     gene            complement(3661..5955)
                     /locus_tag="PLF67_08015"
     CDS             complement(3661..5955)
                     /locus_tag="PLF67_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOE89991.1"
                     /translation="MNTPTALILHGHFYQPPRENPYTGRIETQPSAEPFNDWNEYITA
                     DCYQANTHSRYLNGYGQVLSMTNNYKYLSFNFGPTLLSWLRTNHPCTYEAILQADRES
                     LARLGHGNAIAQAYNHPILPLQKRHDIQTQVMWALEDFHTRFGRESEGLWLSETAINP
                     VTIDVLAENGVKFVILSPWQAKETEKEGLLNGKPAPYGKPFLLTGEKGGTITAFFYNH
                     TLAEGISFGHYLRDADVLYKLLLEIKKKEKSPLIHTATDGEVYGHHEPYGDMAFAALI
                     KKCEAGGELTFTNYGAYLAANPAKEWAVLHDGEEKKGSSWSCSHGVSRWYKDCGCYTG
                     GNDSWNQKWRTPLRASFDALAKRIDEIYISEASKMLGSEETARKVLNSFAPVASHLVP
                     LHEFLAPYTTDEEEITALGCLLQGQKYKHYSYTSCGWFFNDLAGIEPKQNIIYALMAA
                     KLYESYDPLLTSTLLKNLEKAKANEKEDGNGKTIALELMKLLPGKVEAALYYILNRTL
                     ALPSDQQDHYGFFRLDRFKEEEGLFALSITNEQCLQKHQITARMGQGKYSLTVDSNPY
                     SLTASEIPHRMRRELLEQIDKRICTVDEDQIIRLDQSLIHYSLLAQSAPEVLEPVYQQ
                     LVGWAISMIKGLFLFERIRFWEHYRERFLRVLDFFTVYGKAEEIQLLGELFDQVLFNV
                     AEKIQRNGLFEQSIRFVSEFLEEVRKRGFQPDLTKVQDALYPYIVGERLLEVEEDVER
                     VSALARDLNFDISAIDHLTAIERE"
     gene            6032..6739
                     /gene="deoD"
                     /locus_tag="PLF67_08020"
     CDS             6032..6739
                     /gene="deoD"
                     /locus_tag="PLF67_08020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOE89992.1"
                     /translation="MSIHIVTEKQLVASDVLLPGDPLRAEHIAKTFLTDLVHYNTIRG
                     MNGYTGFWNGHRISVQGTGMGMPSFSIYAHELIDAYGAKRLIRVGTCGTMSEELNLRD
                     ILIAQASDCDSGMNPNRFGLYQFAPTATYALLEKAVQTAKKLNLNAVVGTVFTSDQFY
                     DEKAEEKNALAMKAGALAVEMESAELYTLARLKRIQALTLLTVSDSILTGEQVPPKER
                     QNTFDAMIELALHTLFS"
     gene            7304..>7646
                     /locus_tag="PLF67_08025"
     CDS             7304..>7646
                     /locus_tag="PLF67_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE89993.1"
                     /translation="MKQIAYGMVGGGPGAFIGDVHRKAIRMDGLAVLKAGSFSRDAAK
                     SKAHGKTLGVSEDRLYDDYREMARKEGSREDGIKFVVVVTPNSTHFEVCKAFLEQGIH
                     VVCDKPLTWKIE"
BASE COUNT         1774 a         1917 c         2191 g         1764 t
ORIGIN      
        1 gccgatggta ctgccaatag gtgggagagt aggtagctgc cgggcctctt ctttttccta
       61 tgcggtcctt ccttcggaag ggcttttttt taccctgcag actcgctata cttggctctg
      121 gaggctttcg tggcaatcat tcccttctat aaaccaacgc ttcgccgaaa agacatggat
      181 gccgtgcttc agacgatggt cgatgaacgg atcggtcccg gggagcgaaa aaatctcttt
      241 ctgaaagaat tctgcgatcg gatcggcttt gcccatggtg ttgcgctgcg cacggtcatc
      301 gatgccatga tctgcggttt gaagatcttg aaccttcctg aaggaagccg aatcggaacc
      361 ggtgtgctgg ctccttcgta ctatgaggat gtcgttcgat cgctgggata tgagctcctc
      421 cttggagata tcgacaaaga gcacggctgc ctcagccaag cggaggcgga tcggctcgtc
      481 caagaggggt gcaaggctct tctgctccac gagccggtct gtcaaattcc ctttggagtc
      541 gactataaag agcttggtgt tcccgtgatg gaagatatca gccaaagcat cggatcctca
      601 ttcgaagaac ggaacgcggg atcgtgggga gatctggtag tctgttcctt tgaggaggat
      661 ggtgtggttt cctgcggggg aggagccgcc ttggtgttca acgaaaagga gcataaaacc
      721 gcggctaccg agctgttgtc caatacgggg agccatgtcg agctgccgga catgaatgcg
      781 gctctcggct tgattcagct cgccgccctt gatgaacacc ttcatcgccg aagggagctg
      841 tattcgctct acaccaaatc cctgatgaaa acccatcacc ggctcttcgg aatcgggcac
      901 atcgactttg aacccaatgg atacgggttt tgcaccacct tggattcgaa agcggaggat
      961 gtcatcgcct tcgcaaaaaa atacgaagtc tcggcaaaac gaacctttgc cgactcgttg
     1021 gcctcacatc atcaagaggc gtttgaaacg tatccggtgg ctctgccggc atttcatcga
     1081 agcatctccc ttcccatcta cccgtttttg agtcaaagtg atctgcaagt gattttgaaa
     1141 gtaattgccc acctgccgta aggaggtttt gatgagttcg aaacaagtac aaaaggtgtt
     1201 gatcgtagca aactggggaa aagaggaagc tcattccctc agcgcgacga tcaaatccta
     1261 tatcgaagag aaaggaatcg aatgcacgat atggcggacc accgtcgagc aggacaagcc
     1321 gatcgtggcg gaggacaccg atctggtgat ctgcctcggc ggggatggaa cggttctcta
     1381 ttgcgcccgc cacctgcaaa ccctcggtat tccgattttg gcgatcaact tgggtacctt
     1441 cggctacatc actgaaatct cggtcaatga atggaaggaa gcggtcgatc gcttcctgga
     1501 gggctcctac accctcagcc gccgactgat gatccgcgtc ggagtctatc ggaagggaga
     1561 acgggtcttc accgcccatg gcctcaatga gatcgtcgtc tcctcctcgg gaatcagcaa
     1621 ggtgatcggg atgagcctca aggtggggga gactcctgcc gggtacttca gggcggacgg
     1681 aatgatcatc gccactccga ccggctcgac cgcctacagc cttgcagcgg gcggaccgat
     1741 tcttgatgcg gagctctcaa cgctgctcat cactccgatc tgcccgttca ccctctccaa
     1801 tcggcccttg gtgctcagcg gcgattccct gatcaccctc accatcgccc cgaaccagcg
     1861 gaccgggatc gtcctgtccg ttgacggcca gcagaacttt cctctccaag agggtgatga
     1921 gctgattgtc gaaaaatcgc ggagcaggac cctcctcgtc acaagcgaaa agcgcaatta
     1981 catcgaagta ctccgagaga agcttaactg ggcgggagga gctgcgcatg cttgagcgat
     2041 tgcagatcca taattacgcg cttatcgagg atcggcagat tgaatttgaa gagggattta
     2101 ccgtcatcac cggggagacc ggcgccggaa aatcgattat tctcggcgcc ttgtcgatgc
     2161 tgatggggga caaggtggaa agccacgtca tccgcagcgg ttcggagagc gccacggtga
     2221 acgcttcatt cagctttgaa gaaggactcg atgaagagct ccggcgcttt ctctccgagc
     2281 gggatatcga ccttggggat gacgagctcc tgctctctag gacgatcaga agcaacggcc
     2341 gttcctccgc cctgattcaa gggaggatgg ttccccggac cgatttgggt gaacttgctg
     2401 cccagctttt tgaaatcagc gcccaacggg accatcagag tctcctgaaa agcgaagccc
     2461 agcgcaatgc tctggatcgg agcggggagt gtcaggggga acgggcgagc tatcaggaag
     2521 agtggagccg gctccagctc cttaaaaaag agcttgccga gcggagagcc ctctttgaag
     2581 ccgccatgcg cgaggaggag tttcttcgct tcgccgagga ggagctcagt gcgatcgatc
     2641 cgaaagaagg ggaggatgag ctccttaagg gggagattca tcggctctct tcctttgaac
     2701 agatctatga atcattgacg agaagcagtt cgttgctcca ctcctttgaa gcctcaagcg
     2761 ttcttgccgc gctgagagag gcaaaacatg agctggagcc ggcgatcaaa gcggatcgca
     2821 gcttggaaga cttcgccaat cggctggaag agagcgcgat tgaagttcag gatatctatg
     2881 agagcctgcg cgatcatctt tcttcattaa ccttcagcga ggaggaactc gaccgccttc
     2941 agggacggct tgcgcagatc cagcggctga agaagaagta cggtccttct ttggaacggg
     3001 tattggagtt cagagaagag atcgccgaga agctctcgat gcgcgaggac ggagaaatcc
     3061 acctcagcag actggaaaag aagatcgccg agcaggagca tctcctgctt gaagccggaa
     3121 aagcgttgcg gaccaaacgc aagacggcgg caacgcgtct ggcgaagaac gtggagagta
     3181 ggcttcgaac gctcgggatg aaggaggcgg tcttcgaaat ccgcttcagg gagattcctc
     3241 ccgcccccca tgggcttgat gagatctcat tcatgatctg cgccaacccg ggtttggatc
     3301 ttcacccgat cgatgaagtc gcctccggag gggagctttc acggatcatg ctggccctca
     3361 aggtgacgct cgcccggcag ggggagctgc cgactctgat cttcgatgaa gttgatgcgg
     3421 gaatcggagg ggcggtggcg gtaagcgtgg cgaaggagct tgccgagctg ggcaaaaccc
     3481 atcaggtgat cgtgatcacc cacctcgcct cgattgccgg caaggcggat gtccacctga
     3541 cggtgagcaa ggtcgtcgag gcgggaatga gccactcccg catcaagcgg gtggaaggcg
     3601 aggaacgagt ccgtgagata gcacgaatgc tcagcggcga cagcgcacgt gaggaatccc
     3661 tcactcacgc tcgattgctg ttaggtgatc gatagccgag atatcaaagt tcagatcgcg
     3721 ggcgagggcg ctgacccgct cgacatcctc ttcgacctcc aagagccgtt cgccgacgat
     3781 gtagggatag agggcgtctt ggactttggt gagatccggt tggaagcccc gcttgcgcac
     3841 ctcttcgaga aattcggaaa cgaagcggat cgactgttcg aagagtccat tgcgctgaat
     3901 cttttcagcg acgttaaaga gtacttgatc gaatagttct ccaagcagtt ggatctcttc
     3961 ggcttttcca tagacggtga agaaatccaa gacccgtaaa aagcgctccc tgtaatgttc
     4021 ccagaagcgg attcgctcaa agaggaagag tcccttgatc atgctgatcg cccatccgac
     4081 aagctgttga tacaccggct caagcacctc gggggcgctt tgagccagaa ggctgtaatg
     4141 gatcaggctt tggtcgaggc ggatgatctg atcctcatcg acggtgcaga tccgcttgtc
     4201 gatctgctcc aaaagctccc ggcgcattcg atgaggaatc tccgaggcgg taagcgagta
     4261 ggggttggaa tcaaccgtca gcgaatactt gccttgcccc atccgagcgg tgatctgatg
     4321 tttttgcaga cactgttcat tggtgatcga caaggcgaag agcccttctt cctccttgaa
     4381 ccggtcgagg cggaagaacc catagtgatc ctgttgatcg ctcggcaggg cgagggttcg
     4441 gttcagaata taatacaatg ccgcctcaac tttgccgggc agaagcttca tcagttcaag
     4501 ggcgatggtt tttccgttgc catcctcttt ttcgttcgcc ttcgccttct caaggttttt
     4561 caagagggtg gaggtgagca gcggatcata cgattcgtag agtttcgccg ccatcagggc
     4621 atagatgatg ttttgctttg gttcaatgcc cgccaggtcg ttgaaaaacc atccgcagct
     4681 ggtatagctg tagtgtttat acttttgccc ttgaagcagg cacccaaggg cagtgatctc
     4741 ctcttcgtcg gtggtatacg gtgcaaggaa ttcatgcaag ggaacgagat gagaagcgac
     4801 cggagcaaag ctgttcagaa ccttccgggc ggtttcttca ctgccaagca tcttgcttgc
     4861 ttcactgatg tagatctcat cgattcgttt ggcaagcgca tcaaacgagg cgcgcagggg
     4921 ggtccgccat ttttggttcc acgaatcgtt tcccccggta taacacccgc aatccttgta
     4981 ccatcgggaa acaccgtggg agcacgacca ggaagatcct tttttctctt caccatcatg
     5041 caggaccgcc cactccttcg ccggattggc ggccaggtag gcgccgtagt tggtaaacgt
     5101 caattccccg cccgcttcac attttttgat cagggcggcg aacgccatat ccccgtaggg
     5161 ttcgtgatgg ccgtagactt caccatcggt ggcggtgtgg atcaatgggg atttctcttt
     5221 ctttttaatc tccagcagga gtttatacag cacgtccgca tccctgagat agtgcccgaa
     5281 gctgatccct tcggcgaggg tgtggttgta gaagaacgcg gtgatcgttc cgcccttttc
     5341 accggtaagc aaaaacggtt ttccataggg agccggtttg ccgttgagaa gtccctcctt
     5401 ttccgtctct ttcgcctgcc aggggctgag aatgacgaat ttcactccgt tttcggcgag
     5461 cacgtcgatc gtcacggggt tgatcgccgt ttcactgagc caaagtccct ccgattcccg
     5521 tccgaaccgg gtatggaagt cctccagcgc ccacatcacc tgagtctgaa tgtcgtgcct
     5581 cttctgcaag ggcagaatgg ggtggttgta cgcttgtgcg atcgcattgc cgtgacctaa
     5641 gcgagcgagg ctctccctgt ccgcctggag gatcgcttca taggtgcagg gatggttggt
     5701 tctcagccac gaaaggaggg tgggaccgaa gttgaaggaa agatatttgt aattattggt
     5761 catgctgagc acttgcccgt agccgttgag gtagcgggag tgggtgttcg cctggtagca
     5821 atccgccgtg atgtactcat tccaatcgtt gaacggttcg gcggaaggtt gtgtttcaat
     5881 gcgtcccgta taggggtttt ctcttggtgg ttgatagaag tgaccgtgta gtatcaaagc
     5941 agtaggtgta ttcatatggt gcggagtata tcataagggg ccgaagcgtg catagcttgc
     6001 aaaaagggtt cggctcggag agaatgccga tatgagtatt cacattgtaa ccgagaaaca
     6061 gttagtcgct tccgatgtct tgttgccggg agatcccttg cgggcggaac atatcgccaa
     6121 aacgtttctg acggatctcg tccactacaa cacgattcgg ggaatgaacg gctataccgg
     6181 gttttggaac ggacaccgca tcagtgttca ggggaccggg atggggatgc cttccttttc
     6241 gatctatgcc catgagctga tcgatgccta tggagcgaaa cgcctgatcc gggtgggaac
     6301 ctgcggtacg atgagcgaag agctgaacct tcgcgacatc ctgatcgctc aggcctccga
     6361 ctgcgacagc gggatgaatc caaaccgctt cggtttatat cagttcgccc cgaccgccac
     6421 gtacgcgctt ttggaaaaag cggtccaaac ggcaaagaag ctcaacctga acgcggtggt
     6481 gggaacggtg ttcaccagtg atcagttcta tgatgagaag gcggaagaaa agaacgcgct
     6541 ggcgatgaaa gcgggggcgc tcgcagtcga gatggagagc gccgagctct atacgctggc
     6601 ccggttgaag cggatccaag cccttacgct gctgaccgtc agcgactcga tccttaccgg
     6661 ggagcaggtt cctccaaagg agcggcagaa cacctttgac gcaatgatcg agctcgcctt
     6721 gcatacgctc ttttcctgaa agaaacaaca aagcgggtat ggtcggtttg cggtatgggt
     6781 gaaggtaacc gttattgaag gagtagggtc gacgctcaag gtgatcttgc cggaagcggc
     6841 ggtcatttcg gtcaggaagc tgcgaggatg aaggatccgg acttctgtgc aagtattcga
     6901 tgagcccttt tcccgaggct catctgtata atacaggaac ctctgacgta tgtcaatatg
     6961 ggtattaaga taatagtata aaataggata aaaatgttaa aaatactata aaatgcttaa
     7021 tataaagcat ttactattct taataattac aatatatatg acatatttaa tacaatacct
     7081 gctattattc aaaaataaaa ctttgctgaa atggtttttg taagaatata tttgagagtg
     7141 atgacttaag tttgcgccaa accggttccc cctttcttat gcaattggag tacgaataca
     7201 ttttttttgc tctccatatg tcaatattac atatgaatca tcaatcttgc ttatggaaat
     7261 ctctttccag tgtgatacgc tttagataac aaatggaggg gttatgaaac aaattgccta
     7321 tggaatggta gggggaggtc ccggtgcgtt catcggtgat gtccaccgca aagcgatcag
     7381 gatggatgga ctcgccgttt tgaaagcggg ttcattcagt cgggatgcag ccaagtcaaa
     7441 agctcatggc aagacccttg gagtgagcga agaccgactg tacgacgatt accgcgagat
     7501 ggccaggaag gagggctcac gcgaggacgg aatcaagttc gttgtcgtcg tgactccgaa
     7561 cagcacccac tttgaagtat gtaaagcctt tctggaacag ggcatccatg tagtctgcga
     7621 caagccgttg acgtggaaaa tcgaag
//
LOCUS       DAOSLI010000065         7503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9023_length_7503_cov_21.414474, whole genome shotgun sequence.
ACCESSION   DAOSLI010000065 DAOSLI010000000
VERSION     DAOSLI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9023_length_7503_cov_21.414474"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            537..1139
                     /locus_tag="PLF67_08030"
     CDS             537..1139
                     /locus_tag="PLF67_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE89994.1"
                     /translation="MALIRNMVAQDLPYVYDIALKTAFAGLDGTPYYHDRWVVGHYYA
                     APYFFFEPEVCFTALNDRGKPSGFIVGTGDTRSFNRWMQNEWLPPLREYYKGLTEFKS
                     AAERNTVRQLLKGPGEGLWQNLGYPAHLHIDLLGTLQGKGLGKALMHTFFSALQAKDV
                     HGIHLGVDGRNVKAHGFYEAMGFSVLEEVGGSAIFGIKLA"
     gene            complement(1136..2806)
                     /locus_tag="PLF67_08035"
     CDS             complement(1136..2806)
                     /locus_tag="PLF67_08035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE89995.1"
                     /translation="MDIQFYGANRYVTGSCTLVTGNGKKILIDCGLPQGNDAKIGGLQ
                     LPFNAKDIDYLLLTHAHIDHSGRIPLLIKDGFRGTIYATEATKELCSIMLADSAHIQE
                     SEAQWQNRKAKRSGQALVEPLYTVNDAYKSLSRFETIEYEEKRTIDEGITIRYQDAGH
                     LLGSALIELWLTEGAETRKLVFTGDLGNLDQPLIKDPVYITEADFVVMESTYGNRNHA
                     PMIDKDGNTITSLYRARELAAIIQRTFDRGGNVIIPAFSVGRTQELLYLLRIIIEEGL
                     CSETVPVFVDSPLSVKATKVFAGNLYGYMDEETMALVNAGINPILFEGLTPVVDVNDS
                     IALNHRKESCVIISSSGMCEAGRIKHHLKHNLWRRECTVIFSGYQASGTLGRSIYDGA
                     RHVTIFGDQIDIRCEITELHGTSGHADQQGLIRWLSRFEGEIQRVFVVHGDEEIAPWF
                     ASYVQRKLDRDAYAPCFGERFDLLDAESIPKAEPITLTELPYMKDLASALEALSKGES
                     AFGKVLARIKQAAEHTSDEKRSVRLANATRRLASDLEDLAKKWNSDAS"
     gene            2934..3614
                     /locus_tag="PLF67_08040"
     CDS             2934..3614
                     /locus_tag="PLF67_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOE89996.1"
                     /translation="MDEHLYTIRMKSVQRTIEQLRKNNMQAHFIPTIAQVKTEVKARL
                     SKGVTVAVGGSVSLAEAGILELLQSGDYAFLDRYAPNLTGEDIRQIYTASFAADVYLS
                     SVNAITEHGELYCVDGTGNRVAALLYGPKEVIIVASWDKIVPDLAQAVLRVKHIAAPA
                     NATRLKKNTYCTEQGHCISAKLDSENLMALRAGQCPETICASYVVLSNQRIKDRITVL
                     IVGESLGY"
     gene            3626..4816
                     /locus_tag="PLF67_08045"
     CDS             3626..4816
                     /locus_tag="PLF67_08045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE89997.1"
                     /translation="MVSNFTYYTPTEVVFGKNTEREAGRLVKHHGGTKVLLHYGSNSA
                     KRSGLLDRVEASLKAEGIEYVELGGVVPNPRLSKVHEGIELGKKEGVDFILAVGGGSV
                     IDSAKAIAYGLYNGGEVWDFYEGKRTLKGALPHAAIVTIAAAGSEMSNSSVITNEDGW
                     LKRGLNTDYGRCRFAIMNPELLYTLPEYQTMSGIVDIMLHTMERYFIIEDSMSLTDDI
                     AAALLKSVMHYAHILLKDPKNYEARANIMWAGSLSHNGLTGAGHGSRGDWSCHQMEHE
                     LGGLYDVAHGAGLSALWDSWARYVYKNKIERFASFGYEVFDLPKSGDTDADALAAIAA
                     TKAFFESIRMPTTIKALGVNPTEEEILDMADKCSFGDTRTTGSVKALDRNDMEAIYRL
                     ALGD"
     gene            complement(4813..5796)
                     /locus_tag="PLF67_08050"
     CDS             complement(4813..5796)
                     /locus_tag="PLF67_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE89998.1"
                     /translation="MNQIVAFNDQERVLVASCRAVLEEQHKQLAQYLDDRVADLHSLA
                     GIITRSASLVSDLGFSKSRRDLNTLAAKLVNQGVEEVVNLPVRASIGRTFMVAKLHLF
                     GFLLNVVAKQGYLSDEQEEEILSCYHSILFALMAEDLYITIISDANGQESWAKRATHD
                     LVMMWEERSAVSTIAFAPLLQELWDARHLLIPVLGTLMGTVELLQLSMGLSRTWHDFF
                     AAHGQEEQTVLALNEFLFSLSYEQIIALEGLMKQEGYEVVSREGAQDLLQLKPAQRLE
                     GRNEEERSAVRLYRSFLRRNTLAKVRRDSERPGPRSTLEQQFMLYLWSIEH"
     gene            6260..6850
                     /locus_tag="PLF67_08055"
     CDS             6260..6850
                     /locus_tag="PLF67_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE89999.1"
                     /translation="MDKPDYADRAVTEALVNALIHRDYILLGSEIHIDMFDDRLEIQS
                     PGGMYDGRAIQDRDIHTIASVRRNPVIADLFHRMKFMERRGSGLTKILSETAKLPGYD
                     DRLKPEFFSTPSDFRVVLKNVNYSPLANTEQVTVQDDRMKTLIEFCSIPRLRDEMQAS
                     IGIGHREYFRKAFLKPLLESGRLQMTQQPQSKICRC"
     gene            6911..7246
                     /locus_tag="PLF67_08060"
     CDS             6911..7246
                     /locus_tag="PLF67_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90000.1"
                     /translation="MVPVAANVIHLLDDARQAMRELDLVCRPGGTTIIPTYIGRKGEN
                     RGHRFTTMAGRIGAGFRKTFTNASYRRFFVDAGYPDATYILIEGFIPCPIAVIGKKGS
                     DEHDAEPAW"
BASE COUNT         1773 a         2031 c         1961 g         1738 t
ORIGIN      
        1 tcatccgatg ctgacaattt tcattgccct tcatcttcga aattgactgg aagccctatc
       61 ggtcttcctc ttctccaatc tcactgttct tttcctaacg ctatactgtc aataaacatc
      121 ccacctctcg gagggggcat attagaatac ctgaactcat tgaatgggtc aagggcccct
      181 tgccctttct gccaatacga gctttggtgc tcaagccaac aagaaaggat attatagagt
      241 attgaacctt ttgtccatac ccattttgct ctttgagcaa ctatttgtta gtttattata
      301 ctataaatag ttaaaaattg ccagacagcg gtttggtgcc gaattggcct tcatgatgat
      361 acggccgccc tcattgtcaa aacggatcgt aaaaagctta tcactcctcc ccgcccccct
      421 tttaaccgac tttgctccat ccgatcccgt tcaatcactc acaaaccaat cgattatgtt
      481 cttattgtga aaccgggtga attttagtag tgtttgatat ataggaggtg tcaacgatgg
      541 cactcattcg taatatggtc gctcaggatc ttccttatgt gtacgatatt gcactgaaaa
      601 ccgcatttgc agggttggat ggaacacctt attaccatga caggtgggtg gtcggacact
      661 attacgccgc accgtatttt ttcttcgaac cggaggtctg tttcaccgcc ttgaacgaca
      721 ggggcaagcc ctccggcttc atcgtcggaa ccggtgatac ccgttccttc aaccggtgga
      781 tgcagaatga gtggctccct cccctgaggg aatactataa ggggctcacc gagtttaaaa
      841 gcgccgctga acgaaacacg gtcagacagc tcctcaaagg ccccggcgag gggctctggc
      901 aaaacttggg ctacccggct catctccaca ttgacctgct cggcacgctc cagggcaaag
      961 gtctcggcaa ggcgctgatg cacaccttct tttcagcgct ccaagccaag gacgtgcatg
     1021 gaatccacct tggagtggat ggtcgcaatg taaaggcaca cgggttctac gaagcgatgg
     1081 gcttctcggt ccttgaagag gtcggcggca gcgccatctt cggtatcaag ctcgcttagg
     1141 aggcatcact gttccacttc tttgcaagat cctccagatc gcttgccaaa cgccgcgtgg
     1201 cgttcgccaa gcggaccgag cgcttctcat cgctcgtatg ctcggccgcc tgcttgatcc
     1261 gtgccagcac cttgccgaac gcgctctcgc ctttggagag agcctcaaga gctgaggcca
     1321 gatccttcat atacggcagc tcggtaaggg taatcggttc ggccttcgga atcgactcgg
     1381 catcaaggag gtcgaagcgc tccccaaagc agggcgcgta cgcgtcccgg tcgagctttc
     1441 gctgcacata ggaagcgaac cacggagcga tctcctcatc cccgtgcacg acgaagactc
     1501 gttgaatctc cccttcaaag cgggaaagcc atctgatcag cccctgctgg tcggcatgac
     1561 cgctggtgcc gtgcagttcg gtgatttcac atcggatatc gatctgatcg ccaaagatcg
     1621 taacatgacg tgctccatca tagattgaac gcccaagcgt accggaagcc tgatagccgc
     1681 tgaagatgac cgtgcattcc cgccgccaca ggttgtgctt caggtggtgc ttgatccgcc
     1741 ccgcttcaca cattccgctg gaagagatga ttacacagct ttccttgcgg tggttcaggg
     1801 cgattgaatc attcacatca acgaccggag tcaacccttc aaaaagaatt ggattgattc
     1861 ccgcattcac caaggccatc gtctcctcat ccatgtaccc gtacaggttt cctgcaaaaa
     1921 ccttggtcgc cttgacggaa agcggactgt cgacgaagac gggaaccgtt tcggaacaaa
     1981 gcccttcttc gatgatgatc ctcagcagat agagcagctc ctgggtgcgc cccacgctga
     2041 aggcggggat gatgacgttt ccgccgcgat cgaaggttcg ctggatgatc gctgcgagtt
     2101 ccctcgcccg atacagcgag gtgatcgtat tgccatcctt gtcgatcatc ggagcgtgat
     2161 tcctgttccc gtaggtggat tccatgacca caaaatccgc ttcggtgatg tacaccggat
     2221 ccttgatgag gggttggtcc agatttccca gatccccggt gaaaacgagc ttgcgggtct
     2281 cggccccctc ggtcagccac agctcgatga gcgccgagcc gaggaggtgg cctgcatcct
     2341 gataacgtat ggttattcct tcgtcgatcg tccgcttttc ttcatactcg atcgtttcaa
     2401 accgggaaag gctcttatat gcgtcattca ccgtatacag cggttcaaca agtgcctggc
     2461 cgctccgttt ggcttttctg ttttgccact gagcctccga ttcctggata tgagcagaat
     2521 cggcgagcat gatcgaacac agctctttcg tcgcttcggt ggcgtaaatg gtccctcgaa
     2581 acccgtcctt gatcaacagc ggaatccgcc cgctatgatc gatgtgcgca tgggtgagaa
     2641 ggagataatc gatatctttc gcgttgaagg gtagttgaag ccccccgatc ttcgcatcgt
     2701 ttccctgagg caggccgcaa tcgatcagaa ttttctttcc gttgcccgta accaaggtgc
     2761 agctgcccgt cacatatcgg ttcgctccat aaaattgaat atccatagtt gactccttgc
     2821 cttataggat acaccgtatc atagttcgac acccattgta aaaatctcta aaaaggaaga
     2881 aaacttgcaa ggcgacggaa gaacagggta cacttgagaa ggaggatact cttatggatg
     2941 aacatctcta tacgattaga atgaaatcgg tccaacggac cattgaacag ctcaggaaaa
     3001 acaacatgca ggcgcacttc atccccacca tcgcacaggt gaaaaccgag gtgaaagccc
     3061 gtctctcgaa gggagtcacg gtagccgtcg gaggttcggt cagtctggct gaagccggaa
     3121 tcctggagct gttgcagagc ggtgattacg cgttcctgga ccggtatgcg ccaaacttga
     3181 ccggagagga tatccgacag atctacaccg cctctttcgc ggcggatgtc tacctgtcca
     3241 gcgtgaatgc gattaccgaa catggcgagc tttactgcgt cgacggcacc ggcaaccggg
     3301 tcgccgccct gctctacggt cccaaagagg tcattatcgt agcctcctgg gataagatcg
     3361 tccccgatct cgcccaagcg gttctgcggg taaaacacat cgcggcgccc gcaaacgcca
     3421 cccggttgaa gaaaaacacc tactgtacgg agcagggaca ctgcatcagc gccaaacttg
     3481 attcggaaaa cctgatggcc ttaagggccg gacagtgccc ggagacgatc tgtgccagct
     3541 atgtggtgct gtccaatcag cgcatcaagg atcgaatcac cgttctcatc gtcggcgaga
     3601 gcttgggata ttgatggagg aatctatggt ttcaaatttt acctactaca ccccgaccga
     3661 agtggtcttc gggaagaata ccgagcggga ggcgggccgc ctcgtcaaac accatggcgg
     3721 aacgaaggtt ctgcttcatt acggctcgaa ttcagctaaa cgatccggcc tgctggatcg
     3781 agtcgaagcc tccttgaaag cggaaggaat cgagtatgtc gagctgggcg gcgtcgtccc
     3841 caatccgcgg ttgtccaagg tccatgaagg cattgagctt ggaaaaaagg aaggggtcga
     3901 tttcatcctc gccgtcggtg gcggttcagt catcgattcg gccaaggcga tcgcctatgg
     3961 actctacaac ggaggcgaag tctgggattt ctatgagggc aaacgcaccc tcaagggagc
     4021 cctgccgcat gccgcgattg ttacgatcgc cgccgcgggt tcggagatga gcaactcttc
     4081 ggtaatcacc aatgaggatg gatggctgaa acggggtctc aacaccgatt atggacgctg
     4141 ccgctttgcg atcatgaacc ccgagctgct ctataccctg ccggaatacc agacgatgag
     4201 cggaatcgtt gatatcatgc ttcacacgat ggaacgctac ttcatcattg aagactcgat
     4261 gtcgctgacc gacgatatcg ctgccgcttt gctcaaaagt gtcatgcatt acgcccatat
     4321 cctgttgaaa gatccgaaga actacgaggc gcgagccaat atcatgtggg cgggttcgct
     4381 atcccacaat ggcctgacgg gcgccggaca cggctcccgc ggtgactggt cctgccacca
     4441 aatggaacat gagcttggag gactctacga tgtcgcccac ggtgccggac tttcggcgtt
     4501 gtgggacagc tgggcgcggt atgtgtataa gaacaagatc gaacgcttcg cctcgttcgg
     4561 ctacgaagtc tttgatcttc caaagagtgg cgacaccgat gccgacgcgc tcgctgcaat
     4621 cgctgcgacg aaggcgttct ttgaatcgat caggatgcct accacgatca aggctctcgg
     4681 cgtcaatccg acggaagaag agatcttgga tatggcggac aagtgttcgt tcggtgatac
     4741 tcgaacaacc ggttcggtca aagcactcga ccggaatgac atggaggcga tctatcgcct
     4801 tgcgttggga gattaatgct cgatgctcca gagatagagc atgaactgtt gttccaaggt
     4861 ggagcgggga ccgggtcgtt cactgtcccg gcgaaccttg gccagcgtat ttcgacggag
     4921 gaaggagcgg tacaggcgaa cagcggaccg ctcctcttca tttcttcctt ccaagcgttg
     4981 ggccggcttc agctgcagaa gatcttgggc accttcccgg ctgaccacct catatccttc
     5041 ctgcttcatc aacccttcca gcgcaataat ctgctcgtag gaaagggaga aaaggaattc
     5101 gttcaacgct aaaacggtct gttcctcttg gccgtgcgct gcaaaaaagt catgccacgt
     5161 cctgctcaag cccatggaaa gctgaaggag ttcgacggtt cccatcaatg ttccgagaac
     5221 cggtatcaac aaatggcggg catcccacag ctcctgaagc aacggtgcaa acgcgattgt
     5281 cgatacagcg ctgcgctcct cccacatcat gacgaggtca tgggtggccc gcttcgccca
     5341 ggactcctgg ccgttggcgt cactgatgat cgtaatatac agatcttcag ccatcagggc
     5401 gaaaagaatc gagtgataac aagagagtat ttcctcttcc tgctcgtcgc ttagataacc
     5461 ttgctttgca actacgttca aaagaaagcc aaacaggtga agctttgcaa ccatgaaggt
     5521 gcggccgatc gaggcgcgga ccggaaggtt caccacctcc tccaccccct ggttcaccag
     5581 ctttgccgcc agggtgttca aatcacgtct ggatttgctg aatcccaagt cgctcaccag
     5641 tgaggcggag cgggtgatga tgcctgcaag ggagtggagg tcggcaactc gatcatccag
     5701 gtactgggcc aactgcttgt gctgttcctc cagcaccgct cgacagctcg ccacaaggac
     5761 ccgttcctga tcattgaatg ccacaatttg attcacaccc gcctccttca tccagtaagg
     5821 acacaatacg aaaagagggg ccgtttgtaa agggaagagg ttctccggca aaaccttgaa
     5881 cagctgaaag ccgcgtatcc aaggatagaa gcaatgagtg ctctcagtct gccatcgaaa
     5941 ccttaacaca ccgaaggttt ttcggcacgt ttagggactt ttcaggagct atggctgtca
     6001 tgtcctaggg caggaaggaa aaagaaactg tttctctcca taccgttggg ttgatgggat
     6061 ccccagtata gagtcttacc ttatacttcc acccagttag attccaacca tcgccgtata
     6121 atatcttcaa tttcaatgtt acggaaagta agcgaccaat aaagagtatg aaggcagcct
     6181 gatcagtctg ctacacaaca gcagtgagtt cgtgcgcaac aactccaaga ttcgcttcgt
     6241 gaagaaggcc cgatctcgtg tggacaaacc cgactatgcc gatcgggcgg tcactgaggc
     6301 tcttgtaaac gcgttgatac atcgtgacta catcctgctg ggtagcgaga tccatatcga
     6361 catgtttgat gaccggcttg agatccagag ccccggaggc atgtatgatg gtcgagcgat
     6421 ccaagatcgt gatatccaca ccatcgcttc tgtcaggcgc aatccggtga tcgcagatct
     6481 gttccaccgt atgaaattca tggaaagacg tggtagcggg ttgacgaaga tcctcagcga
     6541 gacagcgaag ctccccggtt atgacgatcg tctgaagccc gagttcttct ccacaccttc
     6601 ggacttccga gtggtgttga agaatgtgaa ttactctcct ttggccaata ccgagcaagt
     6661 taccgtgcaa gatgatcgaa tgaaaacgct tatcgagttt tgctcaatac ctcggttaag
     6721 agatgaaatg caggcatcca tagggattgg gcatcgtgaa tacttcagga aagcattcct
     6781 taaaccacta cttgaatcgg ggagattaca gatgacccaa cagccccaat caaaaatatg
     6841 tcgctgctga agcccacttt tcgcaccctg atgtgacaag taaccactga attatttatg
     6901 ctgatttttg atggtgccgg tcgcggccaa tgtcatccac ctactggatg acgcccgtca
     6961 agcgatgcgt gagcttgatc tggtctgtcg cccgggcggc acgaccatca tcccgacgta
     7021 tatcggcagg aaaggagaga atcgcggaca taggttcacg acgatggccg ggcggatcgg
     7081 agcgggcttt cgcaagacgt tcacgaatgc atcctaccgg cggtttttcg ttgatgccgg
     7141 ctatcctgat gccacataca tcctgataga gggattcatt ccctgcccga tcgccgtgat
     7201 cgggaagaaa ggatccgatg agcatgatgc ggagccggcg tggtgagagt tctctgattt
     7261 cattgtccgg acgatataaa gtgacctcca agtttgtaaa tcgtggattc accgtgtgcg
     7321 gctaccgcat acaaatggag gtcaccgggg acagtatgat cgacttcgga agcaatgtcc
     7381 ataccatatc tcatgagatg gtctgtttgt tttactccat gaagagctgg atcacctcat
     7441 cggcatcttt cgcctgttcg agcatccggc ggaagattcc gctggagcag gatcgggcga
     7501 tcg
//
LOCUS       DAOSLI010000066         7408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9168_length_7408_cov_28.101455, whole genome shotgun sequence.
ACCESSION   DAOSLI010000066 DAOSLI010000000
VERSION     DAOSLI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9168_length_7408_cov_28.101455"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1385
                     /gene="hisS"
                     /locus_tag="PLF67_08065"
     CDS             81..1385
                     /gene="hisS"
                     /locus_tag="PLF67_08065"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOE90001.1"
                     /translation="MKDQEIIQPRVLKGFRDSLPSAQIPKEALVSTLQKHFSSYGFVP
                     IDTPALEYMEILLGKGGGETDKQIFHFTDQGGREVALRFDLTVPFARYLAQHEHELAL
                     PFKRYHIDKVWRGENPQKGRYREFTQCDFDIVGVDNAESDAEILSLMASSFEKMNVKH
                     ARFHVAHRGMFNTLLGNLGVENLSVDILRLVDKLRKIGEQETEEQLRALVKNERTARA
                     ILSYISKEDEKEPFETTLDRLAALLGGENEHTLRMRTIHSYLKAEGIEERFLFDPSIT
                     RGLDYYTGIVYETFLEGSEQIGSVCSGGRYNDLASLYTKTHLPGVGSSIGLDRLLAAL
                     EGAYDYRASDVLILNTDKSRYPEAIRVAKQLREAGLKVALFFEPKKLPAQYKWAEANA
                     IPKAIILDRDEEGSFTLRDLGTRTNAVVSIGEAVALLKKEQP"
     gene            1382..3886
                     /locus_tag="PLF67_08070"
     CDS             1382..3886
                     /locus_tag="PLF67_08070"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HOE90002.1"
                     /translation="MIDYKQLPVYQNRQEILDALSKNQVIVVQSPTGSGKTTQMPIIL
                     HEAGYADEKMIGITQPRRIATLSVCDFIKRQLNDRENFIAYKMRFEDTTDYTTRIKVM
                     TDGILLMELKNDPLLMQYSVILVDEAHERSLNIDFILGLLKRIVAQRPEFKVIISSAT
                     INTRVFSRFFDNCPIVSIHSRVYPVHVIYSPAHRQTFDDQLEMIISIVKRESKKQSGD
                     ILIFLSGEFEIVTAVNALYAADPKEELEIYPLYGRLGKEEQERVFTPTSKGKTKVVVS
                     TNIAETSVTIDGITVVIDLGVAKLNFYNQKDFTSSLVPLPTSRSSCEQRSGRAGRTQA
                     GTCYRLFTKEDYQSRPAYQIEEILRTDLSEVVLRMSDLGIYDYEQFSFITRPKAQALK
                     SAEETLRYIGAIDRNRMLTSIGSLMVRFPLLPRHSRVVVEAMMTYPDVLDEVLIAIAF
                     LSTKTPFLFPAGEEDASRAAHRSFNTSAYGDFVMYLNLFRQYANLAKQKQRQDFCTKY
                     YLDHQGMQEIVHVHEQLGEICSEIGFPLSSGGSVREYLSCIAKGLSQYICIRARGTMY
                     KSLSVDQIYIHPGSAWFKTLPRFIIAGEIVQTSRLYARSVSPLEQEWLEDIQPGLSKK
                     LMAFDSKQKGKEEEQEEKSFRKRDQQRGTAGFDLYGKRYPTIKARTKKQQEVVIIPLD
                     DLPSIVKANDRSPQRPKNFRAALAYRGFYVHYNDKFFSLLELNGKLKPELGILDNPPS
                     GVFTIDNARLLIDNLRWLLGFTKSKKNREILGFITLEASGSGNYRFSNNPDFFDALDT
                     SLFHLLHLEDELRDSDRKKEAKEVSSIYTKLLTLAQ"
     gene            complement(3870..4940)
                     /locus_tag="PLF67_08075"
     CDS             complement(3870..4940)
                     /locus_tag="PLF67_08075"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOE90003.1"
                     /translation="MKPLMTVTLAGGAQTEVLLAEDIKDLSAHLGDYGRSVLWVFDSN
                     TNKLFRQLPPARIILDSGEQSKNLTSLERIISLALDEGLGRDGRIIGFGGGVVGDISA
                     LAASLYMRGCRLTLVPTTLLAMVDASIGGKTAVDFQGMKNMIGTFYPANEVLISIDVL
                     KSLSEREYKSGLAEVIKHALLSEDQELFKLLLSRRSEILDREEETLMKLVELSLLVKK
                     SYIERDPTETKGIRKALNLGHTFGHALESSTRFAAFSHGAAVAWGTVRALEAGVALGI
                     TDKKYASSASKLFGSFGYDLDHRIGRGEWIEYKNHLSKDKKKLGGEVQFVLLGGNGKP
                     ELHPLPWNLIQALVMEKAIGRG"
     gene            5029..6165
                     /locus_tag="PLF67_08080"
     CDS             5029..6165
                     /locus_tag="PLF67_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOE90004.1"
                     /translation="MQTTGYFDHAATTFPSAKALERYRAVALEYWANPSTKYPEGQKS
                     HAYLEAMRASIAKSLNVRPNHIIFTGGATEANDIVFQSLLLRLSPGEVIISGIEHSSI
                     IQQQRALEQAGWKIIMLRAPRGFVNPEDLRKALTPKTRLVSIMSLNNAIGTLQEIPAL
                     VRIVREFEKTTGRAIHFHTDATQQLGKLPIDLLSWDVDSASFGAHKFHGPKGVGFLYN
                     TSEGIENLSKGGGQEGGLRGGTENLAGIASMEAALVEALANRKRVTEHVTRLNDLFRE
                     TLESHATILTPKEHASPYIITVSTALPAEVMVRMLIERGFSVSAGSACSSNARAKGVR
                     ILEMMEVAPEHRGGAIRISFSDSNTEEETVSLARAINTIAKESP"
     gene            6162..7379
                     /gene="thiI"
                     /locus_tag="PLF67_08085"
     CDS             6162..7379
                     /gene="thiI"
                     /locus_tag="PLF67_08085"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOE90005.1"
                     /translation="MNTLYVIRLGEIALKGLNRPMFENMLRRNIKAKLKGYRNTITKQ
                     KGRLFLQIGDDCPREVVDTALSTTFGIVGYSRSFSAPKELEAIHEAAREAIGEGPFAE
                     GKGSFKVISRRADKSFPLTSYQLDVELAKTVHEVHPDLTVDVKNPDLILYCEIRDKAY
                     LYTTQKKGLGGLPVGSAGAGMLLLSGGLDSPVAGFLAAKRGVKMDCLYFHAYPYTSDE
                     ALEKVKTLASLIAPYLQGTRLWVAHFTEAQLQIKRTAPENETTLMFRAAMMTLANRHS
                     ERTGSQCIITGEALSQVASQTLEGLSFSDSFSEQLVLRPLIGMDKQEIMDLARKIGTY
                     ETSILPYEDCCVLFSPKHPLTRPDKVVATEHYNAMGIEPFIEEALSNLGVFHFDERGK
                     IIEKNHPTPDPVF"
BASE COUNT         1663 a         2266 c         2079 g         1400 t
ORIGIN      
        1 gatattgcag tgaacgaatg attcgtatta cactctttct acaccatcca acgacaaaac
       61 acaacaggag cagtagggta atgaaggatc aggagatcat ccagccaaga gtcttgaaag
      121 gatttcggga cagtcttcct tctgcccaga ttccgaaaga agcgcttgtg tccaccttac
      181 agaagcattt ttcctcctat gggttcgtcc cgatcgatac cccggctctt gaatacatgg
      241 agattctcct cggaaaaggc ggcggagaaa cggataagca gatcttccac ttcaccgacc
      301 aggggggtcg ggaggtggcg cttcgcttcg atctgaccgt cccgttcgcc cgctatctcg
      361 cccagcacga gcatgagctc gccctgccct tcaaacgcta ccacatcgac aaggtgtggc
      421 ggggggaaaa ccctcaaaag ggccgctatc gggaattcac ccagtgcgac ttcgacatcg
      481 tcggggtcga caacgccgaa agcgatgcgg aaatcctttc cctgatggcg tcctccttcg
      541 aaaaaatgaa cgtcaagcac gcccggttcc acgtggcgca ccgggggatg ttcaacaccc
      601 tgctcggcaa cctcggagtc gagaatctca gcgtggacat tctccggctc gtggacaagc
      661 tgagaaagat cggtgaacaa gaaaccgagg agcagcttcg cgcgttggtg aaaaacgaac
      721 ggaccgcccg ggcgattctt tcatacatct ccaaagagga tgaaaaagaa ccttttgaga
      781 ctacgctcga tcgccttgcc gctctccttg gcggggaaaa tgaacacacc ctccggatgc
      841 ggacgatcca ctcctatctg aaagccgaag ggattgaaga gcgctttctc ttcgatccct
      901 ccatcacccg ggggctggac tactataccg gcatcgtcta tgagaccttc ctggaaggtt
      961 cggagcagat cggctcggtc tgcagcggcg gacggtacaa cgaccttgcg tccctctaca
     1021 ccaagaccca cctgcccggg gtcggctcct cgatcggcct cgatcggctc cttgccgccc
     1081 ttgaaggtgc gtatgactac cgggcaagcg acgtgctgat cctcaacacc gacaagagcc
     1141 gctacccgga agcgatcagg gtggcgaaac agcttcggga agccggcctg aaggtcgccc
     1201 tcttttttga accgaagaaa ctccccgccc agtacaaatg ggccgaagcc aacgcgattc
     1261 ccaaggcgat catcctcgac cgggatgagg aaggatcgtt caccctccgg gatctgggca
     1321 ccaggaccaa tgcggtcgtc tcgatcggag aagcggtggc gttgttgaaa aaggaacagc
     1381 cttgatagat tataagcagc tgccggtata ccaaaaccgg caggaaatcc ttgatgctct
     1441 ttccaaaaac caggtgatcg tcgtccaaag cccgacgggc agcggaaaga ccacccagat
     1501 gccgatcatc cttcatgaag cggggtatgc cgacgagaag atgatcggca tcacccagcc
     1561 gcgaagaatc gcgaccttga gcgtgtgcga tttcatcaag cgccagctga acgacaggga
     1621 aaacttcatc gcctacaaga tgcgcttcga ggataccacc gattacacca cccggatcaa
     1681 ggtgatgacc gacgggatcc tcctgatgga gctgaagaac gatccgctgc tgatgcaata
     1741 ctcggtgatc ctcgtcgacg aagcccatga gcgaagcctg aacatcgact tcatcctcgg
     1801 cctgctcaag cggatcgtcg cccaaaggcc ggagttcaag gtcatcatct cgtcggcgac
     1861 gatcaacacc cgggtcttct cccggttctt cgacaactgc ccgatcgtct cgatccacag
     1921 ccgggtctat ccggtccatg tcatctactc ccccgctcac cgccagacct tcgacgacca
     1981 gctggagatg atcatctcga tcgtcaagcg ggaatcaaag aagcaatcgg gggatatcct
     2041 catcttccta agcggcgagt ttgagatcgt cacggcggtc aatgcgctct atgccgccga
     2101 tccaaaggag gagctggaga tctatccgct gtacgggcgg cttggaaaag aggagcagga
     2161 gcgggtcttc acccccacct ccaaagggaa gacgaaggtc gtcgtttcca cgaacatcgc
     2221 cgagacgagc gtgacgatcg acgggatcac cgtcgtcatc gacctcgggg tggcgaagct
     2281 caacttctac aaccagaagg acttcacctc gtctctggtg ccgctgccca ccagccgttc
     2341 cagctgcgag cagcgaagcg gccgggcggg ccggacccaa gcggggacgt gctaccgcct
     2401 cttcaccaaa gaggattacc agagccggcc ggcctatcag atcgaggaga tccttcggac
     2461 cgacttaagc gaagtcgtgc tgaggatgag cgatctggga atttatgatt atgaacagtt
     2521 ctccttcatc acccgaccga aagcccaggc tctgaagagc gccgaagaga cgctgaggta
     2581 tatcggagcg atcgacagga accggatgct cacgagcatc ggatcgctga tggtccgctt
     2641 cccgctcctg ccccggcaca gccgggtggt ggtcgaagcg atgatgacct atcccgacgt
     2701 cctggacgaa gtcctgatcg cgatcgcctt cctctcgacg aagacgccgt tcctcttccc
     2761 cgccggcgag gaggacgcaa gccgtgcggc ccaccgctcc ttcaacacct ccgcctacgg
     2821 cgatttcgtg atgtacctca atctcttcag gcagtatgcg aacctcgcaa aacaaaagca
     2881 gcggcaggat ttctgcacga aatactacct cgatcatcag ggaatgcagg agatcgtcca
     2941 cgtccatgag cagctggggg agatctgctc ggagatcggc ttcccccttt caagcggcgg
     3001 gtcggtcagg gaatatcttt cctgcatcgc caaaggcttg tcgcagtaca tctgcatccg
     3061 ggcccgcggc acgatgtaca agagtctttc cgtcgaccag atctacatcc atcccggctc
     3121 ggcgtggttc aagaccctgc cccggttcat catcgcaggc gagatcgtcc agacctcccg
     3181 cctctacgcc cggagcgtca gcccgttgga acaggagtgg ctggaggata tccagccggg
     3241 cctttccaaa aagctgatgg cgttcgactc caaacagaaa gggaaagagg aggaacagga
     3301 ggagaaatcc ttcaggaaac gggaccagca gcgcggcacc gccggcttcg acctctacgg
     3361 caagcggtat cccacgatca aggcccggac caagaagcag caggaggtgg tcatcatccc
     3421 cttggatgac cttccttcga tcgtgaaggc gaacgaccgc tcgccccaaa ggccgaagaa
     3481 cttccgggcg gctctcgcct accggggatt ctacgtccac tacaacgaca agttcttctc
     3541 gctcttggag ctgaacggaa agctcaagcc cgaactcggg attctggaca atccgccttc
     3601 cggtgtcttc acgatcgaca acgcccgcct cctcatcgac aatctcaggt ggcttctggg
     3661 cttcaccaaa agcaagaaga accgggagat cctcgggttc atcacccttg aagccagcgg
     3721 cagcggaaac tatcgctttt cgaacaaccc cgacttcttc gatgcgttgg acacctcgct
     3781 ctttcatctg ctccatctgg aggatgaact gagggatagc gaccggaaga aggaggcgaa
     3841 ggaagtctct tcgatctaca cgaagctgct taccctcgcc caatagcctt ttccatcacc
     3901 agcgcttgga tcagattcca cggaagcgga tgcagctcgg gcttgccgtt cccgcccaag
     3961 agaacgaatt ggacctctcc gccaagcttc ttcttgtctt tggagaggtg attcttatat
     4021 tcgatccatt cgccccgccc gatccgatga tcgaggtcgt acccgaagga gccgaacagc
     4081 ttggaggcgg aggaagcgta ctttttgtcg gtgatgccga gggcgacacc cgcttcaagg
     4141 gcccggacgg tgccccaggc gaccgcggct ccgtggctga aggcggcgaa ccgggttgag
     4201 gactcaagcg cgtggccgaa ggtatgcccg aggttcaatg cttttcggat tcctttcgtt
     4261 tcggtgggat cccgttcgat gtaggatttc ttgaccagaa gcgaaagctc gacgagcttc
     4321 atcagcgtct cctcttcacg gtcgaggatc tcggaccttc gcgagagcag aagcttgaag
     4381 agctcctggt cttcgctgag cagggcatgc ttgatgactt ccgccagccc gctcttgtac
     4441 tcccgctcgc tcaaggactt cagcacgtcg atcgagatga ggacttcgtt cgccgggtag
     4501 aacgtcccga tcatgttctt catcccctgg aagtccacag cggtcttgcc cccgatcgag
     4561 gcgtcgacca tcgcaagcag cgtggtggga acgagggtca gcctgcaccc ccgcatatag
     4621 agggaggcgg cgagagcgga gatgtcaccg acgacgcccc cgccgaagcc gatgatgcgg
     4681 ccgtcccggc cgaggccttc atcgagggcg agggagatga tccgctcaag gctcgtaagg
     4741 ttcttcgatt gctctccgct gtccaggatg attcttgcgg gaggaagctg gcgaaagagt
     4801 ttgttggtgt tggagtcgaa gacccacaga accgaacggc catagtcgcc taggtgcgcc
     4861 gacaggtctt tgatatcctc ggcaagaaga acttcggttt gggcgcctcc ggcgagcgtg
     4921 acggtcatca gtggtttcat acccctattt atacccttaa agcccgttcg cttacaaccg
     4981 tcaatccttc ccttgaacaa tcaccatcgt ttcagcacaa tgagaaccat gcagaccacc
     5041 ggttatttcg atcatgccgc caccaccttt cccagtgcaa aagcccttga gcgctaccgg
     5101 gccgtcgctc ttgaatactg ggcgaaccct tccaccaaat acccggaagg gcaaaagtcc
     5161 catgcctacc tggaggcgat gagggcttcg atcgcaaaaa gtctcaatgt ccggccgaac
     5221 cacatcatct tcaccggcgg ggcgaccgag gcgaacgaca tcgtctttca atcgcttctt
     5281 ctgcgcctga gtccgggtga agtgatcatc tcgggcatcg agcattcctc gatcatccag
     5341 cagcagcggg ctcttgagca ggcgggctgg aagatcatca tgctccgagc cccccggggg
     5401 ttcgtcaacc cggaagacct gagaaaggcc cttaccccga aaacccgcct cgtctcgatc
     5461 atgagcctga acaacgccat cggaacgctt caggagatcc ccgctcttgt caggatcgtc
     5521 cgtgaatttg agaagaccac cggccgagcg atccatttcc acaccgatgc gacccagcag
     5581 ctcggaaagc ttccgatcga tctcctctcc tgggatgtgg acagcgcctc cttcggcgcc
     5641 cacaagttcc acggtcctaa gggggttggc ttcctctaca acacctccga aggaatcgaa
     5701 aacctctcca aaggcggcgg acaggagggg ggcctgaggg gcggaaccga gaaccttgcc
     5761 gggatcgcaa gcatggaggc cgccctcgtc gaagcgttgg ccaaccggaa aagagtgacg
     5821 gagcatgtaa cccgcctcaa tgacctgttc cgtgaaacct tggagtccca cgcaacgatc
     5881 ctgaccccga aagaacacgc ttcgccctac atcatcaccg tctccaccgc ccttccggcg
     5941 gaagtgatgg tgcggatgct catcgagcgc ggcttcagcg tctccgcggg aagcgcctgc
     6001 tcctccaacg cccgcgcaaa aggggtgcgg atcctggaga tgatggaggt cgcccccgag
     6061 catcgggggg gagcgatccg gatcagcttt tccgattcca acaccgaaga agagaccgtt
     6121 tcccttgccc gggcgatcaa tacgatcgcc aaggagagtc catgaacacc ctgtacgtca
     6181 tccgactcgg cgagatcgcc ctcaaggggc tgaaccgccc gatgttcgag aacatgctcc
     6241 gccggaacat caaggccaag ctcaaagggt atcgcaacac gatcaccaag cagaagggcc
     6301 gcctctttct tcagatcggc gatgactgtc cccgggaggt ggtcgacacc gccctgtcga
     6361 cgacgttcgg catcgtcggc tacagccgct cattcagcgc ccccaaggaa ttggaggcga
     6421 tccatgaagc ggccagggag gcgatcggtg aaggtccgtt tgccgaaggc aaaggctcct
     6481 tcaaggtgat cagccggcgg gcggacaaga gctttccttt aacgagctac cagctggatg
     6541 tcgagctggc aaaaacggtc catgaggtcc atcccgacct caccgtcgat gtgaaaaacc
     6601 ccgatctgat tctgtactgc gagatcaggg acaaggcata cctgtatacg acccagaaga
     6661 aaggcctcgg cgggcttccg gtcggcagcg ccggggcggg gatgctcctt ctcagcgggg
     6721 ggctggacag cccggtggcg ggatttttgg ccgccaagcg gggggtgaag atggactgcc
     6781 tctacttcca cgcctacccg tacacaagcg acgaagctct tgaaaaggtg aagaccctcg
     6841 cctccctcat cgccccctat cttcagggaa cgcgcctgtg ggtggcgcac ttcacggagg
     6901 cccagctcca gatcaagcgg accgccccgg agaacgagac gacgctgatg ttccgggcgg
     6961 cgatgatgac cctggccaac aggcactccg aacggaccgg cagccaatgc atcatcaccg
     7021 gcgaggcgct gagccaggtg gcgagccaga ccctcgaggg gctttccttc agcgactcct
     7081 tcagcgagca gctggtcctc cgcccgctca tcgggatgga caagcaggag atcatggacc
     7141 tggcgaggaa gatcggcacc tatgagacct cgatccttcc ctatgaggat tgttgcgtcc
     7201 tcttcagccc caagcacccc ctcacccgtc cggacaaggt ggtcgcgacg gagcactaca
     7261 acgcgatggg aatcgagccg ttcatcgagg aggcgctctc aaacctgggg gtgttccact
     7321 tcgatgagcg gggaaagatc atcgagaaga accaccctac ccccgatccg gttttttgag
     7381 tatctttatc ctatgaagat tgtaagac
//
LOCUS       DAOSLI010000067         7337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9283_length_7337_cov_29.407031, whole genome shotgun sequence.
ACCESSION   DAOSLI010000067 DAOSLI010000000
VERSION     DAOSLI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9283_length_7337_cov_29.407031"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLF67_08090"
     CDS             complement(<1..959)
                     /locus_tag="PLF67_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE90006.1"
                     /translation="MSNYSIILGNLGNTRDRFLSSGYKDELPKETLIRQASEINGVTG
                     VELVGTWDVTSENVEQVGEMLDKHGLKCVSIIPDHFAQKRWGRGTLSAKDEDIRKQAL
                     DYTFECVEMARTLGCKIINLWPGQDGYDYPLQSNFVRERRWLLENITKVAKATPDIRF
                     SLEYKPKEPRNFSFMARASDTLLLAKETGLDNVGVTIDTGHAFVAGENVSEAVVILQE
                     YGKKLFHMHFNDNYGSWDDDMIVGSVHFPWYVELLFWLKETGYEGWLSMDQYPYREDG
                     QGALRESVEFLQMIESRLTPAVMEEIRELVTKGDAVASQRWLR"
     gene            complement(975..1988)
                     /locus_tag="PLF67_08095"
     CDS             complement(975..1988)
                     /locus_tag="PLF67_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE90007.1"
                     /translation="MGIKEIAAEAGVSKTTVSLALNGHKGVSDETRTRILEIARRMNY
                     RLPSERIVHQESKGFIMFARLSKHGLILNRDQSSFIVDYIDSINDAVTKAGYTFEILD
                     HTFTSVSALTELLSSRKPKGIIVLGTELDEDDVHNLQSLPFTFVVIDTQFESACCDFV
                     DMANIAALAKIVNHLIDRGHKRIGMISCTTPSGNIILRERGFSLALERAGLSPASFLR
                     IQPGFKGAYNDMKTYLKQENQLPEAFFCYNDVAALGVMKALKEHRIRIPEDVSIIGFD
                     NLPMSSMSEPRLTTIKVPNRLIGTIATELLVKKLSTKHQKESITIQVNGTLVVRDSVM
                     DRR"
     gene            2222..3229
                     /locus_tag="PLF67_08100"
     CDS             2222..3229
                     /locus_tag="PLF67_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE90008.1"
                     /translation="MKRVLVFLLIALFAVGLVFAAGAKETKSDDYEIVVVPKDASNPW
                     FVRMKVGVDEYAKETGLNVYQRGTPQIDATLQAQLVQDLIAQGVDAICVVPVDLESID
                     PVLAQARKAGIVVITHEGADLENVDYDIEAFSNTGYGAFIMDNLAEAMGGEGLYTTMV
                     ASLTNGSHNEWADAGVAHQKAKYPKMTLLEADKRVESFDNGDVAYNTAKELFKKYPNL
                     KGIMGTSSYDAPGVARAIEELGLVGKAFTSGTGMPLDNAELLKSGVVKSLTLWDPALA
                     GKAMIALAVKVLNGEQITGPVNLNVEGYEALQFKTGSKTVLEGEGWIVINAANVYDFG
                     F"
     gene            3329..4813
                     /locus_tag="PLF67_08105"
     CDS             3329..4813
                     /locus_tag="PLF67_08105"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE90009.1"
                     /translation="MAETLLRAQSIYKSFVGVQALKDVSFSIKRGEIHCLAGENGSGK
                     STLIKIISGVYTPDSGHIEFDGHQYSKITPIEAINLGIQVIYQDFSIFPNLTVMENLA
                     INTELAANRKFVNWKRIRRVAEEALAKINVRIDLDAYVGSLSVAEKQIIAISRALMFN
                     TKLIIMDEPTTALTKKEVRNLFAIILQLKERGIATLFVSHKLNEVFEISENFTILRSG
                     ELVATGSTKELDDKKFSYYMTGRTFESRRFTPETVTKEPIFEADRVGIPGSFKDVSFA
                     LHGGEIIGITGLLDSGRTELALSMFGMKPIREGEFRVDGTKVELTTPREAIRHGIGYV
                     PEDRLSEGLFLPQPIGDNIVITELDSLTKRFGVVDTDRRDEEIRTWVKNLAIATPNPD
                     NACQTLSGGNQQRVVLAKWLANNPRILVLNGPTVGVDIGSKHDIHTILQELANDGMGI
                     IIITDDLPEVVENCSKILVMKSGKIVAKLDAEEADEQKILAYML"
     gene            4827..5870
                     /locus_tag="PLF67_08110"
     CDS             4827..5870
                     /locus_tag="PLF67_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE90010.1"
                     /translation="MKGGTPTLLKKLTRRNEFYVFLVLLVLCLLIEVRSGQFFSPNNL
                     VDIASAMIVPGLFAIGAFLVIVSGGVDVSFPALASLTMYATTKMLLDANYQGGAALPI
                     AIALVIGAVLGAFNGLFIGYFKLPAMIVTLGSSSVFKGLMQGALNSKQLTVIPPGMRR
                     WGTTALFIARNPVSGLTSRMYVTFIAFIAVLALVFFLLRFTMFGRGIYAIGGSEVSAH
                     RAGFNVIRTKVIMYVLVGMIASLAGIIRASMMQQAHPTNMLGMEMNIIAGVVLGGTAI
                     TGGRGTLTGCMLGTLLIVIVENSMILLGIPTSWRSVFTGVLIIVGTGVSAYQTIVMNR
                     SKRVQKAEKGVAA"
     gene            5867..6886
                     /locus_tag="PLF67_08115"
     CDS             5867..6886
                     /locus_tag="PLF67_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE90011.1"
                     /translation="MTTPTMLKKAQSIYRSDRHVWRLIIMITLWMIFMAITRFRKFYT
                     LINFQTMAAQFPEFGLMSLGIMLAMITGGIDLSVVGTANLTSILMAFLLINLTTATGG
                     LPAYAIPLVFILAILLGAAFGLFNGLLISRFNIPPILATMGSGELFTGICMAMTNGNA
                     ISKFSRTYARTINNRLFDMIPVQLIIFTVMAVIIWFLLTKTAYGSKLYLLGTSPTVAR
                     FSGLKTRQLLMQTYMISGICAALGGMIMLANYNSARADYGTVYTLQCVLIVVLGGVSP
                     VGGRGKISGVVLAIILLRLLETGLNRFPKISSYYISLIWGGVLILVMVLNYFTENAKV
                     KTASI"
     gene            6895..>7337
                     /locus_tag="PLF67_08120"
     CDS             6895..>7337
                     /locus_tag="PLF67_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOE90012.1"
                     /translation="MKKIRVLLAGESWVTFSTHHKGFDRFAAGMYDNGQRYLEAALKS
                     DPSIEYVHMPGHEVSERFPFTMEELKEWDVIIISDIGSNSFLLSNKVFLEGKKEANRL
                     QLLKEWTSGGGALVMCGGYLSFSGFQASAKFFRTPIEEVLPVDIYT"
BASE COUNT         1662 a         1951 c         2035 g         1689 t
ORIGIN      
        1 tggcggagcc agcgctggga agcgactgcg tcccctttgg tgacaagttc gcggatctcc
       61 tccatgacgg ccggggtgag gcggctttcg atcatctgaa ggaactccac gctttcccga
      121 agagcgccct gtccgtcttc ccggtagggg tactgatcca tcgacaacca gccctcgtat
      181 ccggtctcct tcagccagaa gagcagttcg acgtaccagg ggaagtgaac cgaaccgacg
      241 atcatgtcgt cgtcccatga tccatagttg tcgttgaagt gcatgtggaa gagcttcttc
      301 ccatattctt gaagaatcac gaccgcttcg gagacgtttt cccccgccac gaatgcgtgt
      361 cccgtatcga tcgtcacccc cacgttgtcc agtccggtct cctttgcgag caggagggtg
      421 tcggacgccc gggccatgaa gctgaaattc ctcggttcct tcggcttgta ttcgagggaa
      481 aaccgaatgt ccggtgtagc cttcgccacc ttggtgatgt tctcgagaag ccaccttcgt
      541 tcccggacga aattcgactg cagcggatag tcgtatccat cctgccccgg ccacaggttg
      601 ataatcttgc acccgagcgt tcgagccatc tcgacgcatt caaaggtgta gtcgagcgct
      661 tgcttccgga tatcctcatc cttggcggaa agcgtaccgc gtccccagcg cttctgggcg
      721 aagtggtcgg ggatgatcga gacgcacttc aggccatgct tgtccagcat ctctccgacc
      781 tgctcgacgt tctccgaggt cacatcccat gttcccacga gctcgactcc ggtaactccg
      841 ttgatttccg aagcctgacg aatcagtgtt tcctttggaa gctcgtcctt gtaccctgac
      901 gaaagaaatc tatcccgggt gttcccgaga ttgcctaaaa taattgaata gttgctcacg
      961 acaatacctc cttgtcatct tctatccata acactgtcac gaacgaccag cgttccattc
     1021 acttggatcg tgatcgactc cttttgatgc tttgttgaaa gttttttaac gagcagttcg
     1081 gttgcaatgg tgccgatgag gcggttgggg accttgattg tggtcagccg gggctcggac
     1141 atcgaactca tcggaagatt gtcgaagccg atgatcgaaa catcctcggg gattcgaatc
     1201 ctgtgttcct tcaacgcctt catcactccc aaggccgcca cgtcgttgta gcagaagaac
     1261 gcctccggca actgattctc ctgtttgagg tacgtcttca tatcgttgta cgcacctttg
     1321 aatcccggtt ggattcgcag aaaggatgcc ggggaaaggc ctgcccgctc caacgccagc
     1381 gaaaatcccc gttcgcgcag gatgatgttt ccgctgggcg tggtgcagct gatcatcccg
     1441 atccttttgt gtccgcgatc gatgagatgg ttgacgatct tcgccagcgc cgcgatattc
     1501 gccatatcga caaagtcgca gcacgccgat tcaaattgag tatcgatcac gacaaacgtg
     1561 aacggcaatg attgaaggtt gtgaacgtca tcctcatcca gctcggtacc cagcacgatg
     1621 attcctttcg gttttcgaga ggaaagaagc tccgtcaatg cggaaacgga ggtgaaggtg
     1681 tgatcgagga tttcaaaggt atagcccgct ttcgttacgg catcgttgat cgaatcgatg
     1741 taatccacga tgaagctgct ttgatcgcga ttgagaatca agccatgttt gctgagtcgg
     1801 gcgaacatga tgaacccctt gctttcctga tgaacgattc gttcggaggg gaggcggtag
     1861 ttcatcctgc gggcgatctc caggattctt gtccgagttt catcgctgac gcccttgtgt
     1921 ccgttcagcg caagggaaac cgttgttttt gatacgccgg cttcagcggc aatctctttg
     1981 atacccatga atgaagcata tgctttgagt tttacgctgt caaatatttt attgaaattt
     2041 tagttgaatg cctctgtatt cacagatatt ccgatattta agaactcaaa aaggcgaaaa
     2101 tatcagctaa acaggaaaga aagatattcc ggtactaaac aatttcatca tatttatttt
     2161 gacatccaca aggtgctggg gtagagttat gctacagact gttgtaaagg aggaacacag
     2221 gatgaaaaga gtactcgttt ttcttttaat cgctttgttt gccgtcggac tcgtatttgc
     2281 cgccggtgcg aaagaaacaa agagtgatga ttatgagatc gtcgttgtcc cgaaagacgc
     2341 atcaaaccca tggttcgtgc ggatgaaggt cggtgtcgat gagtatgcga aagagaccgg
     2401 cttgaatgta tatcagcgag gaacacctca gatcgatgct acattgcagg cgcagttggt
     2461 tcaggacttg atcgcccagg gcgttgatgc gatttgtgtg gttcccgtcg acctcgagtc
     2521 gatcgatccg gttcttgcgc aagcccgaaa agccggaatc gtcgtcatca cccacgaagg
     2581 agcggacctt gagaacgtcg attatgacat cgaagcgttc agcaacaccg gatacggtgc
     2641 gttcatcatg gacaaccttg ccgaagcgat gggtggtgaa ggattgtaca ccacgatggt
     2701 cgcctcactg accaacggtt cccacaacga gtgggccgat gcgggtgtcg cccatcagaa
     2761 agcgaaatat ccgaagatga ccttgctcga agcggacaag cgcgtcgaat cgttcgacaa
     2821 cggagacgtt gcctacaaca cggccaaaga gcttttcaag aagtatccca acctgaaggg
     2881 aatcatggga acatcctcct atgacgcccc gggtgtcgct cgtgcgatcg aagagcttgg
     2941 tcttgtcgga aaagcattca cctccggcac cggaatgccg ctggacaacg ccgagctgct
     3001 gaagagcggc gtcgtcaagt ccctgacgct ctgggatccc gcactcgccg gcaaggcgat
     3061 gatcgccctg gcggtgaagg tcctcaacgg cgagcagatc accggaccgg tgaacctcaa
     3121 tgtcgaagga tatgaagccc tccagttcaa gacgggaagc aagaccgtcc tcgaaggcga
     3181 aggctggatc gtaatcaacg cagcgaacgt ctacgacttc ggattctagt gttgttacta
     3241 tccgattact gtcctgtcaa agactctttg gcaggacagt atcgtgttat actgtagctt
     3301 acagaactac caccacgagg tgatatcgat ggcagagacg ttattacgag cccagtcaat
     3361 ctataagtcg tttgtcgggg ttcaggcatt gaaggacgtg tcgttctcaa tcaagcgcgg
     3421 ggagatccac tgcctcgccg gtgaaaacgg aagcggaaaa tcgaccctga tcaagatcat
     3481 cagcggcgtg tacacccccg attccggaca catcgagttt gatggacatc agtactccaa
     3541 aatcactccg atcgaggcga tcaatcttgg aattcaggtc atctaccagg acttctccat
     3601 cttccccaat ctgaccgtga tggaaaacct cgctataaac accgaactgg ccgcaaaccg
     3661 caagttcgtg aattggaagc ggatccggcg ggtagccgaa gaagctcttg caaaaatcaa
     3721 cgtccggatc gatctggatg catatgtcgg atcattgtcg gtcgcggaaa agcagatcat
     3781 cgcgatcagc cgggctctca tgttcaacac gaagctgatc atcatggacg agccgacgac
     3841 cgccctcacc aaaaaagagg tgaggaacct gtttgccatc atccttcagc tgaaagagcg
     3901 cggcatcgcc acgctgtttg tcagccacaa gctgaatgaa gtttttgaaa tctccgagaa
     3961 cttcaccatc cttcgcagcg gcgagctggt tgcgaccggc tcgacgaagg agctggatga
     4021 caagaagttc tcctactata tgaccggtcg gacgttcgag tcccggcggt tcactcccga
     4081 aacggtcacc aaggaaccga tttttgaagc ggaccgggtc ggtattcccg ggtcattcaa
     4141 ggatgtttcg ttcgcccttc atgggggcga gatcatcgga atcaccggac tgctggactc
     4201 cggacggacc gagctggctc tttcgatgtt cggaatgaag ccgatcaggg agggggagtt
     4261 ccgggtggat ggaaccaagg tcgagcttac cacccccaga gaggcgattc gccatgggat
     4321 cggatacgtt cccgaggacc gcctcagtga agggctcttt cttccccagc cgatcgggga
     4381 caacatcgta atcaccgaac tcgattcatt gacgaaaagg ttcggtgtcg tcgacaccga
     4441 tcgccgtgat gaagagatcc gcacgtgggt taagaacctt gcgatcgcta ctcccaatcc
     4501 cgacaacgcg tgccagaccc tttcgggagg aaaccagcag cgggtggtgt tggcgaaatg
     4561 gctggcgaac aatccccgga ttcttgtcct caacggtccg acggtcggcg tggatatcgg
     4621 ctccaaacat gatatccata cgattctcca agagctggcg aacgatggga tggggatcat
     4681 catcatcacc gacgacttgc cggaagtcgt cgaaaactgc tcaaaaattc tggtgatgaa
     4741 gagcgggaag attgtggcca agctggatgc cgaagaagca gacgaacaga agattctggc
     4801 gtatatgttg tagggaggga gacggaatga aaggcggtac accgacattg ctgaaaaaac
     4861 tgactcgacg aaacgagttc tatgtatttc tcgttctcct tgtgctctgt ttgctgattg
     4921 aagtccgatc gggacagttc ttctcgccca acaacctcgt cgacatcgct tcggcgatga
     4981 tcgttcccgg gctgttcgcg atcggagcgt tcctagtcat cgtttccgga ggggtcgacg
     5041 tttcctttcc ggctcttgcg agcctgacga tgtacgcgac cacgaagatg ctgcttgacg
     5101 ccaactacca agggggagcg gcgcttccga tcgcgatcgc cttggtgatc ggagccgttc
     5161 tgggagcgtt caacgggttg ttcatcgggt acttcaagct tccggcgatg atcgtgaccc
     5221 tcgggtcttc cagcgtgttc aaaggcctga tgcagggagc cctcaactcg aaacagctga
     5281 cggtgattcc cccggggatg agacgatggg gaaccaccgc cctcttcatc gcccgcaacc
     5341 ccgtctccgg cctgaccagc aggatgtacg tcacgtttat cgcattcatc gcggtgctcg
     5401 ccctcgtctt cttcctgctt cggtttacga tgttcggaag ggggatctat gcgatcgggg
     5461 gctcggaggt cagcgcccac cgggcgggct tcaatgtcat ccggaccaag gtgatcatgt
     5521 acgttctggt cggcatgatc gccagtcttg ccggaatcat ccgagccagt atgatgcagc
     5581 aggcccaccc gacgaacatg ctcggaatgg agatgaacat catcgcgggc gtcgtcctgg
     5641 gagggaccgc catcaccggc ggccgaggca ccctgacggg gtgcatgctc ggaacgctcc
     5701 tgatcgttat cgttgaaaac tcgatgatcc tgttgggtat tcccacctcg tggcgaagcg
     5761 ttttcaccgg tgtcctcatc atcgtcggca cgggagtcag tgcctatcag acgatcgtga
     5821 tgaatcgctc caagcgggtg cagaaagcgg agaagggggt agcggcatga ccacaccgac
     5881 tatgttgaaa aaggctcaaa gcatctatcg aagcgatcgg cacgtatggc ggctcatcat
     5941 catgatcacc ctgtggatga tcttcatggc gatcacccgc ttcagaaaat tctatacgtt
     6001 gatcaacttc cagacgatgg ccgcccagtt ccccgagttc ggactgatga gcctggggat
     6061 tatgctggcg atgatcacgg ggggaatcga cctctccgtc gtcggaaccg ccaacctgac
     6121 ctcaatcctg atggcgttcc tgttgatcaa cctcacgacg gcaaccggag ggctgccggc
     6181 gtatgcgatt ccgcttgtgt tcattttggc gattcttctg ggtgcggcgt tcggactgtt
     6241 caacggactc ttgatctcac gattcaatat tccgccgatt ctggcaacga tgggaagcgg
     6301 tgagctcttc accgggatct gtatggcgat gaccaacggg aatgcgatca gcaagttttc
     6361 ccgcacctac gccagaacga tcaacaacag gctgttcgat atgattcccg tccaactgat
     6421 catcttcacc gtgatggcgg tgatcatctg gttcctgctg accaagaccg cctacggttc
     6481 gaagctgtat cttctgggaa cgagtccgac cgtcgccagg ttcagtggat tgaaaaccag
     6541 acagctgctg atgcagacct atatgatctc gggcatctgc gccgccttgg gcggaatgat
     6601 catgctcgcc aactacaact ccgcccgggc ggactatgga acggtctaca ccctccagtg
     6661 cgtgctcatc gtcgtccttg ggggagtgag tcccgtggga ggacggggaa agatcagcgg
     6721 agtcgttctg gcgatcatcc tgcttcggct cctggagacg gggttgaacc ggttcccgaa
     6781 gatcagcagc tactacatct ccctgatctg gggcggtgtg ctgattctgg tgatggtgct
     6841 caactatttc accgagaacg ccaaagtgaa gacggcaagc atataggagg aactatgaag
     6901 aagattcgag tactgttggc cggagagtcg tgggtcacct tttcaaccca tcacaaaggg
     6961 ttcgatcgct ttgccgcagg catgtacgat aacggacagc gatatctgga agccgctttg
     7021 aagagcgatc cttcgatcga atacgtccat atgccgggcc atgaagtgag cgagcggttc
     7081 cccttcacga tggaggagct gaaggagtgg gatgtgatca tcatcagcga catcggatcc
     7141 aactccttcc ttctttcgaa caaggtgttc cttgagggaa agaaggaggc caatcggctt
     7201 cagctgctga aggagtggac ttcaggcggg ggagccttgg tgatgtgcgg cggatatctt
     7261 tcgttttccg ggtttcaagc gagcgcgaag ttcttccgga ccccgatcga agaggtgctt
     7321 ccggtggaca tctacac
//
LOCUS       DAOSLI010000068         7277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9356_length_7277_cov_19.448629, whole genome shotgun sequence.
ACCESSION   DAOSLI010000068 DAOSLI010000000
VERSION     DAOSLI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9356_length_7277_cov_19.448629"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   28..300
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=89..117
                     /rpt_unit_seq="agaaacacccccgcgcatgcggggaagac"
     gene            complement(359..646)
                     /gene="cas2e"
                     /locus_tag="PLF67_08125"
     CDS             complement(359..646)
                     /gene="cas2e"
                     /locus_tag="PLF67_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HOE90013.1"
                     /translation="MVVVIANDLPPAVRGRMKLWFIEPRANVFVSGIKDSVATKVVKY
                     LINNCPPSSGLLVLQRINTPPFYKIWGVGDTSKKIALIDGFQLIFEKIAQE"
     gene            complement(647..1504)
                     /gene="cas1e"
                     /locus_tag="PLF67_08130"
     CDS             complement(647..1504)
                     /gene="cas1e"
                     /locus_tag="PLF67_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HOE90014.1"
                     /translation="MQKPKKRLFIKIERNMLPQISDRYPFLYLERGRVEIDDSSVKWI
                     DSEGNLVRLPSALLQTVLLGPGTSITHEAVKVLTASNTTVCWVGEDSLLFYAVGTTPT
                     SNTRNLRHQSKLASTEKSRLAVARRMFSARFPEEDVETKSLSELMGMEGLRVKAVYER
                     LATEYGVGWKGRSYIPGKFELSDMTNQIITASNVALYALITSTVYHLGYSPHIGFIHS
                     GSPLPFVYDLADLYKEELCIDLAFKLTYEMGGIYNRHTVAEEFRERVIESNLLGRIPK
                     DITSLLGEP"
     gene            complement(1491..2177)
                     /gene="cas5e"
                     /locus_tag="PLF67_08135"
     CDS             complement(1491..2177)
                     /gene="cas5e"
                     /locus_tag="PLF67_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HOE90015.1"
                     /translation="MKYIVLWLEGPLQSWGESSKYGRRETLAFPTKSGVYGMLLAAMG
                     SKGEQEELLAKLAPLPQKVLSFGSSSQMMDFHMIGSGYDSSDPWQKLAIPRKVDGVIP
                     TGTGGSKMTYRYYLQDAKFAVIQGVSDELLDPIVDALKHPVYSPSLGRKSCVPTEFIF
                     QGIYDSQEEAEEHAKKIAGEKCVELAYDVRESSEANPWDLAVQDVPIVFGSHKKYAGR
                     YVEITEYAKT"
     gene            complement(2185..3252)
                     /gene="cas7e"
                     /locus_tag="PLF67_08140"
     CDS             complement(2185..3252)
                     /gene="cas7e"
                     /locus_tag="PLF67_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HOE90016.1"
                     /translation="MNTKYKGMNIEFHILQSFPVTSLNRDDVGSPKTAVVGGVTRARV
                     SSQAWKRQIRSALQDFGISLAIRTKYVADYLCDIMIKDGASKEQAEGASEAIAAILTK
                     DTLHFFSETEAQHLARYAKEKNYTIDASKKPEVSAVLKVHKAALGTGFEHLDGLDIAL
                     FGRMVAQGPDLTLEGASVFAHAISTHKVSNELDFFTALDDVKEEMESEVGSSHMGVIE
                     FNSATYYRYVALDVGQLVENLGGDEHVPQAIEAFIKALYVAVPYARQRTQAGAIPWDF
                     ARVYVRKGQRLQASFENPVKERGEGFSKPSREALVSYLEEKEALSGSLFGKIAECTWG
                     LDQGFSIDDLIRDVIAATKQG"
     gene            complement(3275..3850)
                     /gene="cas6e"
                     /locus_tag="PLF67_08145"
     CDS             complement(3275..3850)
                     /gene="cas6e"
                     /locus_tag="PLF67_08145"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HOE90017.1"
                     /translation="MIASMLTLGPRDIRHLDLTDEYTMHKIVYDLFPGSDRTFLYYQY
                     AFAERRGVKILILSREQPLVPTLGTIESKMVKDSFLRYRYYAFKVRLNPVTRLGGKAT
                     PVTGHDLLTNWFLEKQERWGFVADASRLDLFDVGVATIQKGKNKITLNQCTYTGVLEV
                     VDQDRFIKGFENGLGRAKGFGFGLLQLKPLR"
     gene            complement(3847..4368)
                     /gene="casB"
                     /locus_tag="PLF67_08150"
     CDS             complement(3847..4368)
                     /gene="casB"
                     /locus_tag="PLF67_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HOE90018.1"
                     /translation="MADGKEKAFVSWVLRQKRDENKGLISRLRKAESPATEFQAWEVL
                     ARWVDIEKPWEREAYGLIGASVSRTGKEHDGSLSLGGALRAVALGKGKNIDLQDSSEA
                     LRLRRVVACTSQRELVDILRPILRYLNSKDVALSYERLLKELMTFHFEDAQERTKARW
                     IKDFYTGAEEVRS"
     gene            complement(4371..5870)
                     /gene="casA"
                     /locus_tag="PLF67_08155"
     CDS             complement(4371..5870)
                     /gene="casA"
                     /locus_tag="PLF67_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HOE90019.1"
                     /translation="MNNTYNLLDELWIPITGGRPISLIDAWDVESPRLLGGTAIQKLS
                     VLKLLLAIAQRAYTPADTKEWERLGVSGLAKHCVEYLEKHRSLFFLYGDKPFLQYPIL
                     DALHTKKGEPLPVLPIGRPYHPDIPPANESITNQIQAYRIPTDAEKALFLISVMNHSL
                     GGKRVTHIEPLSSDYPEKSVTAAPGPSIGNYVGYQHTFLLGSSIAETVYINLFTRETL
                     AAFPQWADDPLIPPWEAMPESEDDANARRIQNGFMGTLVGLSRFVLLKDDGALFTEGL
                     QYPSHKQGWREPFMTSIGADRVAFLDISKKPWRNITALLALSLSGKDSGVSCPQIDLL
                     LGRAREVTRRLGIWSGGLKVRAATGDQSVKQDDDFIESTVWFESAVLGDRLFAELENQ
                     MKYLDELGTILYRSVRNYFVKLGMDPKQQSSSYAQQATARYWAFCETVFQQIVDNWNN
                     PESLKRIHGIIRHKVETIYNDTCKKETARQLEAWVLNHPFEGRFVKKEE"
     gene            complement(5874..>7277)
                     /gene="cas3"
                     /locus_tag="PLF67_08160"
     CDS             complement(5874..>7277)
                     /gene="cas3"
                     /locus_tag="PLF67_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOE90020.1"
                     /translation="VLHVRHNAVRSFGLVGKVVILDEIHSYDAYTGALIRYLIKELEA
                     LGSTVIMLSATLTKNRKEELLDESLSNEARSYPLVTSKTEAGIVHSLPRVPEDSLCTT
                     QCTSDYDKAVALARQVALEGGQVLWIENTVLDAQKSFLQFGSWAKEAGIEVGLLHSRF
                     PAYRRSELEDVWVGRYGKDAQNERRGRILVGTQVLEQSLDIDADLLITRLAPSDMLLQ
                     RIGRLWRHRSNDRFRPKGLEKTALIITPDIDGRFNPQVGFGSSGLVYEPYVLIQTYKV
                     WKDLKRIAIPSDVPTILDRTYESNPETEIERKLRADLIRKREELGRKANVGVSMANPT
                     LSEQILTRYSEVGTCPLLLVRNLPAKGSTKWVLLDGSIIDCSLRDDDSKLEIAKRIMQ
                     NIIVGPIYSMPDAQSLSELAWIRPYYYIDEEQESRLRIALLEESGTIRALQGGEANED
                     YDLTYTDHLGFRAVKRR"
BASE COUNT         1741 a         2034 c         1639 g         1863 t
ORIGIN      
        1 gaccgaatgg gagaccgaaa aagctttgga aacacccccg cgcatgcggg gaagactgga
       61 attggtgttg catacacagg ctacaagtag aaacaccccc gcgcatgcgg ggaagacact
      121 ttcctgcccc cttgaatggc aacttaccca gaaacacccc cgcgcatgcg gggaagactc
      181 ccatatgcca cggtagcaca catgactctg agaaacaccc ccgcgcatgc ggggaagacc
      241 agccaacaaa aaaaagaatg ttaaaacttt cagaaacacc cccgcgcatg cggggaagac
      301 actgacatat agtataccat ttattatatc tacgctaggt gtggtggatg ttgattttct
      361 attcctgcgc tatcttttca aaaatcaatt ggaagccatc gatcagggcg atcttttttg
      421 atgtgtctcc gactccccat attttgtaga aaggaggagt attaatacgt tgaagaacga
      481 gcagtcctga ggatggagga cagttgttga tcaagtattt tacaactttt gtggcgacag
      541 aatcttttat cccggaaacg aaaacattcg cacggggctc aatgaaccaa agcttcattc
      601 ttccacgaac ggctggaggt agatcattgg cgatcacgac gaccatttat ggctctccca
      661 atagactcgt aatatcttta ggaatccttc caagtaaatt actttctatt acccgttccc
      721 taaactcctc ggctactgta tggcggttgt agattccccc catttcgtag gtcaatttga
      781 aggcaagatc tatgcataac tcttccttgt acagatcggc taagtcataa acaaacggta
      841 aagggctccc cgagtggatg aatccgatat ggggcgaata ccccaagtgg tagaccgtgg
      901 acgtgatcaa ggcgtataac gccacattgc tagcggtgat gatctgattg gtcatatcgc
      961 ttagctcgaa ctttccagga atatatgacc ttcccttcca accaactcca tactcggtcg
     1021 ccagtcgctc atatactgcc tttactcgta gcccctccat tcccatcagc tctgaaagag
     1081 acttggtttc aacatcttct tccggaaatc gtgctgaaaa catccttcga gccactgcca
     1141 acctcgattt ttccgtcgag gccagtttgg attgatgccg tagatttctt gtattcgaag
     1201 taggagtggt tccaacggca taaaagagca gactatcttc ccctacccaa cagacagtcg
     1261 tgtttgaagc ggttaaaacc ttgaccgcct catgagtgat gcttgtcccc gggcccagca
     1321 aaacggtctg cagcagagct gaaggaagcc tcacgaggtt tccttcagaa tcaatccact
     1381 tcacactgct gtcatcgatc tccactcggc cccgttccaa ataaaggaac ggatacctat
     1441 cggagatctg cggaagcatg ttccgctcga tcttgatgaa taatcgtttc ttaggttttt
     1501 gcatactccg ttatttctac gtatcgacca gcatattttt tatgactacc aaacacgatg
     1561 ggaacatcct gcaccgcaag atcccagggg ttcgcttcac tcgactccct gacatcatat
     1621 gccaattcga cacacttctc tcctgctatc ttttttgcat gctcctccgc ctcttcctgt
     1681 gaatcatata tgccttgaaa gatgaattcc gtcgggacac agctctttct tccaaggctc
     1741 ggactgtaga caggatgttt cagagcatcc acgataggat ccagcaactc atcgctcact
     1801 ccttggatca ctgcaaactt cgcatcttgc agatagtatc gatacgtcat tttcgatcca
     1861 ccggtgcctg ttggtatgac tccatcaacc ttacggggta tagccaactt ctgccaagga
     1921 tcggaagagt cgtatccgct gccgatcata tgaaaatcca tcatttgtga cgaagaccca
     1981 aaggagagga ctttctgggg aaggggcgct agtttcgcca aaagctcctc ctgctcccct
     2041 ttggacccca ttgccgcaag aagcattccg tagactcccg atttcgtcgg gaatgcaagc
     2101 gtctcccttc ttccatactt ggaggactcg ccccaggact gaagcggacc ttccagccat
     2161 aacacgatgt acttcatagt gacctcaccc ttgcttggtc gctgcgatga catcacgaat
     2221 caggtcatcg attgaaaacc cttgatccag accccacgta cattcagcga ttttaccaaa
     2281 caatgatcca gagagcgctt ccttctcttc caaataggac accagagctt ctctacttgg
     2341 tttggagaat ccttcgccgc gctcctttac cgggttttca aacgaagctt gcaatcgttg
     2401 tccctttcgg acatacacgc gggcgaagtc ccacggtatc gctccggcct gagtccgctg
     2461 tcgagcatat gggacggcaa catataatgc cttgatgaac gcctcaatgg cttgaggcac
     2521 atgctcgtcg ccaccaagat tttcaaccag ttgtccgaca tccagtgcca cataccggta
     2581 gtaggtcgcc gagttgaact cgatcactcc catgtgtgaa gaacccacct ccgactccat
     2641 ttcttctttc acatcgtcga gcgccgtgaa gaaatcgagt tcgttggata ctttatgggt
     2701 cgatatcgca tgagcgaaaa cggaagctcc ttcaagagtc agatcaggcc cctgcgctac
     2761 catccgcccg aacaaagcga tatccagtcc gtcaagatgc tcgaaccctg taccaagtgc
     2821 tgctttatga acctttagta ctgcactcac ttccggtttc ttggacgcat ctatcgtgta
     2881 gtttttctcc ttcgcatatc gggccagatg ctgagcttca gtttcgctga agaagtgaag
     2941 ggtgtccttc gtcaggattg cagcaatcgc ctcggaagcg ccttccgcct gctctttaga
     3001 tgcaccgtct tttatcatga tgtcgcagag atagtcagcc acatactttg tccggattgc
     3061 caaggatatc ccgaaatcct ggagagcgct tcgaatctgg cgtttccagg cttggctgct
     3121 cactcgggcc cgggttacgc cgccgacaac cgccgttttg ggacttccga catcatcccg
     3181 gttcaatgat gtgacgggaa agctttgcag aatgtgaaat tcgatgttca ttcctttgta
     3241 cttcgtgttc atgtaagact ccttataata atgcttatcg taacggcttc aattgaagca
     3301 atccaaaacc aaatcctttt gcccgaccca acccattctc gaatcccttg atgaaccgat
     3361 cctggtcgac gacttccaga acgcccgtat atgtacactg gttaagtgtg atcttgttct
     3421 tacctttctg tatcgtggcg accccgacat caaagagatc caacctgctt gcatcggcca
     3481 cgaaccccca ccgctcctgc ttttccagaa accagtttgt cagcagatca tgtcctgtaa
     3541 ccggagtggc cttcccacca agcctcgtga cgggattgag tctcaccttg aatgcatagt
     3601 accggtagcg cagaaaggaa tccttgacca ttttcgattc tatcgtcccg agagtcggaa
     3661 ccagcggctg ctcccgtgaa agaatcagaa tcttcacgcc cctcctctct gcaaacgcgt
     3721 attggtaata gagaaaggtt cgatccgacc ccgggaacag gtcatacacg atcttatgca
     3781 tcgtatattc gtcggtcaaa tcgagatgcc tgatgtcacg tggcccaagc gtcaacattg
     3841 aagcaatcat gatcggacct cctccgcccc ggtgtagaag tctttgatcc accttgcctt
     3901 cgtccgttcc tgggcatctt caaagtgaaa tgtcatcagt tccttaagaa gacgttcata
     3961 ggaaagagcg acgtccttgg aattaagata gcggagaatt ggtcgcagaa tgtcgacaag
     4021 ttcccgctgg cttgtgcatg ccaccaccct tctgagccga agcgcttccg agctgtcctg
     4081 aaggtcgata ttctttcctt tgcccaatgc caccgcccga agggctccac ctaagcttaa
     4141 tgacccatca tgttcttttc cggttctgct tacactcgcc ccgatgagtc catacgcttc
     4201 acgttcccaa ggcttttcga tatccaccca gcgggcaagc acctcccaag cctggaattc
     4261 ggtcgccggg ctttccgctt tccgcagtct ggagataagt cctttgtttt cgtcacgctt
     4321 ttggcgcaac acccaggaaa caaatgcctt ttcttttccg tcagccatct ctactcctcc
     4381 ttcttcacga atcgtccctc gaagggatga ttaagcaccc acgcctccaa ctgccgggcc
     4441 gtctctttct tacaggtgtc gttataaatc gtttcaacct tatgccgaat gattccatgg
     4501 attcgtttca gactttccgg attgttccaa ttgtcaacaa tctgttggaa cacagtttca
     4561 caaaaggccc aatatctagc cgtagcctgc tgagcatacg aactggattg ttgttttgga
     4621 tccattccca gttttacaaa atagttccgg acagatcggt ataaaatggt acccaactca
     4681 tcaaggtatt tcatttggtt ttccagctcg gcaaacagcc tatcaccgag gacagccgac
     4741 tcaaaccaaa cggtggattc tatgaaatca tcatcctgtt ttaccgactg gtctccggtt
     4801 gccgcccgaa ctttcaaccc cccgctccaa atccctaggc gtcgagttac ctcccgtgcc
     4861 cgtcccaaaa gcagatcgat ttgcggacag gagacaccac tgtcctttcc actgaggctc
     4921 aaggccaata acgcggtgat gttccgccac ggttttttgc tgatatctag aaatgctacc
     4981 ctatctgcac cgatgctcgt catgaacggt tcccgccatc cttgcttatg gctgggatac
     5041 tgaagccctt cggtaaaaag tgctccatcg tctttgagca gtacgaaccg gctcaatcca
     5101 accagcgtcc ccatgaaccc gttctggatt ctccgagcat tggcatcatc ttcactttcg
     5161 ggcatcgcct cccaaggggg tatgagcgga tcgtctgccc actggggaaa cgctgccaag
     5221 gtttcacgag taaacaggtt tatatacacc gtctcggcaa tcgagctacc taaaaggaag
     5281 gtatgctggt accccacata gttcccgatg gaaggccctg gtgctgcagt gacgcttttc
     5341 tctggataat cagaggataa aggttcgata tgtgttaccc gcttccctcc taatgaatgg
     5401 ttcatcacgg agatgagaaa aagagctttt tccgcatcgg tcggaatccg gtatgcttgt
     5461 atctgattgg taatcgattc attggctggt gggatatccg gatgataggg tcttcctatc
     5521 ggcaatacag gcaggggttc tcctttcttc gtgtgcaatg catcgagaat cggatattgc
     5581 agaaaaggct tatccccgta gaggaaaaac agggagcgat gtttttctag atactccacg
     5641 cagtgttttg ccaatccgga aactccaagt cgctcccact ccttcgtgtc ggcgggggta
     5701 taagcccgtt gagcgatcgc caacagaagt ttaagcaccg acagcttctg gatggcggtt
     5761 cctcccagta gccgagggga ctcgacatcc cacgcatcga tgagggaaat gggccttcct
     5821 ccggttatcg gaatccagag ctcgtccaac agattatatg tattgttcac aacctacctc
     5881 cttttcactg ccctaaaacc aaggtgatcc gtatatgtaa gatcatagtc ctcattcgcc
     5941 tcccctccct gcaatgccct gatcgtccca ctctcctcaa gcaaggcgat acgcaaccga
     6001 ctctcctgct cttcatcgat atagtagtac ggacgaatcc acgccagctc ggataaagac
     6061 tgagcgtctg gcatactata tatgggccca actatgatgt tttgcatgat gcgcttcgcg
     6121 atttccagct tggaatcatc gtctcgtaag gaacaatcga ttatgcttcc atccagcaaa
     6181 acccatttcg tggaaccttt cgccggaaga ttgcgcacca acaacaacgg acaggttcca
     6241 acttcggagt atcgggtgag aatctgctcg ctcagcgtgg gattcgccat ggagacccct
     6301 acattagcct ttcgccccag ttcttctctt ttccggatca agtccgcccg tagtttgcgt
     6361 tctatttcgg tttccgggtt cgactcgtat gtccgatcga gaatggttgg tacatcggag
     6421 gggatggcga ttcgcttgag atctttccag actttatatg tctgtataag gacgtagggt
     6481 tcatacacca gaccggaaga cccgaatcca acttgaggat tgaaacggcc atcgatgtcg
     6541 ggagtgatga tcagtgcggt tttttccaag cccttaggtc ggaaccggtc gttcgagcga
     6601 tgtcgccaaa gccttccgat ccgctgaagt agcatatcgc ttggagccag acgggtgatg
     6661 agcaggtcgg catcgatatc aagtgattgc tccaagacct gcgtgcctac aagaatcctt
     6721 cctctgcgct cattctgagc atcctttcca taccggccca cccacacatc ctccagttcg
     6781 cttcggcgat acgcaggaaa acgtgaatga aggagaccca cctcgattcc ggcttctttc
     6841 gcccaagagc caaactgaag gaatgatttt tgggcatcca aaaccgtatt ttcaatccac
     6901 agaacctgtc ccccttccaa tgccacctga cgagcgaggg cgaccgcttt gtcataatcc
     6961 gacgtgcact gcgtggtaca gagcgaatct tcagggacgc gaggaaggct gtgaacaatc
     7021 cctgcttccg tcttgctggt caccagcgga tatgagcgag cctcgttgct caacgattcg
     7081 tcaagcaact cctcctttcg attcttcgtg agggttgcgc tcaacatgat cacggtgcta
     7141 ccgagcgcct caagctcctt gatgagatac cggatcaatg cccccgtgta ggcatcgtac
     7201 gaatggatct catccaggat gacgaccttg cccacaagac cgaaactccg aacggcatta
     7261 tgcctcacgt gcaaaac
//
LOCUS       DAOSLI010000069         7187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9480_length_7187_cov_26.214807, whole genome shotgun sequence.
ACCESSION   DAOSLI010000069 DAOSLI010000000
VERSION     DAOSLI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9480_length_7187_cov_26.214807"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1142
                     /locus_tag="PLF67_08165"
     CDS             39..1142
                     /locus_tag="PLF67_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE90021.1"
                     /translation="MKKVTIILVVLIVGAFLFAAGTKEVAKSDGRPTIRLITDATGID
                     DKSFNAAAWRGITEFYGESVGNTPSRGKLYDVVTSQTQDAYIPNLRDAAEDGSFDLIM
                     VTGFTFADALGEIAPQYPDQKFTIVDVDWVGHPNVMEFIYSEEEGSYLVGLAAALQAK
                     EDGIANPRFGFIGGVPGSTITKFEMGYVQGIRSVFPNAQIIDYYANDWGKPELAKAQA
                     KNWFDSGVYAIFSAAGGTGNGTIAQAKEYRAQGKNVWAIGVDSDQYEEGIYSGTKSAV
                     LTSMLKRVENSSLHVLNAVADGTFKGGVIQMGMVDDGVGYSTANPELSKKVIEAVEKA
                     KADVISGKVKLYKTYKDALAAGAAPAGLAALDD"
     gene            1233..2762
                     /locus_tag="PLF67_08170"
     CDS             1233..2762
                     /locus_tag="PLF67_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE90022.1"
                     /translation="MHEYAIEMRGITKTFPGVIANDKVNLSVKNQEIHALLGENGAGK
                     STLMSILFGAYSADSGTILINGEEVEIKNPNVATALGIGMVHQHFKLVQNYTVTENIV
                     LGMEPRRGQILDLKKARSEVASLSARYGLAVDPDAKIEDISVSQQQRVEILKTLYRSA
                     KIIIFDEPTAVLTPQEIDELIEIIIKLKDEGKTIVLITHKLREIKAVADRCTVLRRGK
                     YIGTVEVADVSEGDLAEMMVGRAVRFEIDKPDQQPGDVVLKLENVSVKDHLGLEKVSN
                     LTLDVHSGEVVGIAGVDGNGQSELLGAITGLLPLSGGEIYINDRPIGNLTIRERIEAG
                     LGYTPEDRQRYGLVADFTIAENAVLKNYYHPRYTGRFGILNDQAMHDEAETLIEQFDI
                     RAGEGSHTKAGSLSGGNQQKVIVAREISLRPKVLIVAQPTRGLDVGAIEYIRKRIIEE
                     RSRGQAILLVSFELDEIMNLCDRIATISKGTIVAVNRSDEVTERDIGMMMAGSRMKES
                     V"
     gene            2759..3853
                     /locus_tag="PLF67_08175"
     CDS             2759..3853
                     /locus_tag="PLF67_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE90023.1"
                     /translation="MKKKDAWIQSDGAVSVLVVLLGFLVGTILVALVGKNPLNMYKAI
                     LQALSGYNIDNGKMNVRYIGETLNYSVPFILCGLSMGFANRVGLFNIGGEGQYIMGMT
                     VAQIIALLGPQIPVLHWLLALLGAMLIGAVWGGVVGILKAKYEVSEVVSTIMLNYVAL
                     YLSRIICLQLPGATTYRTASYPETALITNVFFQKITKNSLLNNGIFFMILAVVAFWFI
                     MEKTSLGYGMRATGFNKEAARASGIPVVRSISISMAISGIFAGLAGGIIALGSFKYGR
                     VIAGMDNYGFDGIAVALVGNSTALGTTLAGLLFGLLKNAQALMQAKQIPKEITFIIQG
                     LIVIFIALRSALRIYKEYLDKRRLQKEVGL"
     gene            3850..4758
                     /locus_tag="PLF67_08180"
     CDS             3850..4758
                     /locus_tag="PLF67_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE90024.1"
                     /translation="MMIWNMLPSVLMIVAPILITAIGGMISEKSGVVNIALEGLMMIG
                     AMSAAAAHVLMEGSGMNHTLSLILAMVIGTVIGGLFSLLHAVAAINLNADQTISGTGI
                     NLLASGVTIFASQILFNADRTREFRMGMQTDALGFYPTAYIALVVVLLSWFVLYKLPF
                     GMHLRACGEHPGAAESVGINVKRIRYIAVLASGMLAGLAGGCVVLTQTIQYTSNTING
                     RGFIALAAVSFGRWRPAGVAGAALLFGTAQAFAIIANNIPALRVLPTEVFNILPYVVT
                     LVALVLFSGKNYAPAAAGKPYEKGAS"
     gene            4758..5459
                     /locus_tag="PLF67_08185"
     CDS             4758..5459
                     /locus_tag="PLF67_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOE90025.1"
                     /translation="MSVHIAAKKVASVVLAPGDPLRAKLVAERFLEKSRLITDIRNIL
                     GYTGIYKGVEISVIATGMGGPSIGIYSYELFNEYEVETIIRIGTCGGFQKEILPGDRI
                     LALTASTDSAWAHQYNLKGTLSPACDPDLLLTAALLDPSAHVGMVFSSDLFSSYNALG
                     EESWKAWAAMGALAQDMETYALYATAAHANKRALSMLTMTDCLATGNALHDDERQSAL
                     YPMIELALEVAYREL"
     gene            5462..6073
                     /locus_tag="PLF67_08190"
     CDS             5462..6073
                     /locus_tag="PLF67_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOE90026.1"
                     /translation="MNFLDIMRHRFACKLYDVDKPLSQEEIDEILEYGRLTPTSFGLE
                     MWHFHAVVSKEKLKELYTACYEQEGVRTSGMTVAILVKTPKHLDPDGSFVYERGIRFP
                     SDIDWFIEDYRPYHEFLKDNHRLEGWLRSQGYLAIANMMTGARSLGIQSCAIEGFDEE
                     GVLRVLGIDPEEWLVSLLCTFGHAAEEERPKIRVPLDELVTCH"
     gene            complement(6070..6993)
                     /locus_tag="PLF67_08195"
     CDS             complement(6070..6993)
                     /locus_tag="PLF67_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOE90027.1"
                     /translation="MKQKVILDVDTGLDDAAALFLAAGAEELEIVAVIATAGNVGLDK
                     TVENTLNVIETTGLSCPVFAGSAKPLIRQPVEAGDFHGESGLDGPVFGKRVIQELQEE
                     DGVSAMIRLVKRHPGEITIISVGPLTDLAHALRREPALPSLVKEIVVMSGSFSGGNVT
                     ENAEFNAYADPEAAAIVFSSGAPLVMLTLDITRTVTLSRERLKYLRTVEGRSAEVFSQ
                     CMDTYMANYEAKKFGWPQMHDPLCVAYVIDRNRFAVEDRRVVVDIDSASPSYGMTSRE
                     AIDGEGGVIIPTDIDRPWFWEMVERSLRNLP"
BASE COUNT         1589 a         2051 c         2102 g         1445 t
ORIGIN      
        1 aacattctat aacggacgaa ccataaggag ggttatccat gaagaaagta acgattatac
       61 tcgttgtcct tattgtcgga gcgttccttt tcgccgccgg taccaaagag gttgccaaaa
      121 gcgatggaag accaaccatc cgattgatca ccgatgcgac gggaatcgat gataaatcct
      181 ttaacgccgc cgcctggcgg ggaattacgg agttctacgg cgagagcgtc ggaaacactc
      241 cctcgcgcgg aaaactgtat gatgtagtta ccagccagac gcaggacgca tatatcccca
      301 accttcgaga cgcagctgaa gacggaagct tcgatctgat catggtcacg ggcttcactt
      361 ttgccgatgc cctcggtgaa atcgctcccc agtaccccga tcagaagttc acgatcgtcg
      421 acgtcgactg ggtcggccat ccgaacgtca tggagttcat ctactcggaa gaagaaggct
      481 cctatcttgt cggtctcgcg gcggctctgc aggcgaaaga ggatggaatc gccaacccgc
      541 gcttcgggtt catcggcgga gtccccggct cgacgatcac gaagttcgag atgggctacg
      601 tccagggaat ccgatcggtc ttccccaacg cccagatcat cgactactac gccaacgatt
      661 ggggcaagcc ggagcttgca aaagctcaag ccaagaactg gtttgacagc ggtgtgtatg
      721 cgatcttcag cgcagcgggc ggaaccggaa acggaacgat cgctcaagcg aaagaatatc
      781 gagctcaggg gaaaaacgtc tgggcgatcg gtgtcgactc cgaccaatat gaagaaggaa
      841 tctattcggg aaccaagagc gccgttctga cctcgatgct caagcgggtc gagaattcct
      901 ccctccacgt cctgaacgcc gttgccgacg gcaccttcaa ggggggagtg atccagatgg
      961 gaatggtcga cgacggtgtc gggtattcga cggcgaaccc cgaactctcc aagaaagtca
     1021 ttgaggccgt ggagaaagcc aaggcggacg tcatcagcgg caaggtcaag ctgtacaaga
     1081 cctacaagga cgctcttgcc gccggcgccg ctcccgcagg cctcgccgca ttggacgact
     1141 gatgttctgt tgccggcgga tttctgccgg catctccttc tgcccgccat cggcgatggc
     1201 gggtagagga agatggaatc aaggagagac gggtgcacga atacgctatc gagatgcggg
     1261 ggataactaa aacgttcccg ggagtcatcg caaacgacaa agtgaacctc agcgtcaaga
     1321 atcaggagat tcatgcgctt ttgggggaaa acggcgccgg taaatcgacc ctgatgagca
     1381 tccttttcgg ggcctacagc gctgacagtg gaacgattct catcaacggt gaagaggtgg
     1441 aaatcaaaaa ccccaatgtc gccacggcgt tgggaatcgg aatggttcat cagcacttca
     1501 aactggttca aaactacacc gtcaccgaga atatcgtctt gggcatggaa ccgaggcgcg
     1561 gtcaaatcct cgacttgaag aaggcgcgga gcgaggttgc ctccctttca gcccggtatg
     1621 gcttggcggt cgatcccgat gcgaagatcg aagacatctc cgtcagccag cagcagcggg
     1681 tcgaaatcct gaagaccctc taccgaagtg caaagatcat catcttcgac gagccgaccg
     1741 cggtgctcac tcctcaggag atcgatgagc tgatcgagat catcatcaag ctcaaagacg
     1801 aaggaaagac gatcgtcctg attacccaca agctgcggga gatcaaggcg gtcgccgacc
     1861 ggtgcaccgt gttgcgccgc ggcaaataca tcggaacggt tgaagttgcc gacgtcagtg
     1921 aaggtgattt ggctgaaatg atggtcgggc gggcggtccg ctttgagatc gacaagcctg
     1981 accaacaacc tggcgatgtt gtcctgaagt tggaaaacgt cagtgtgaaa gaccatctcg
     2041 gactggagaa agtctccaac cttacgttgg atgtccacag cggcgaggtc gtcggaatcg
     2101 ccggagtcga cggcaacggt caaagcgaac tcctcggggc gatcaccggt ctgcttcctc
     2161 tttccggcgg agagatctac atcaacgacc ggccgatcgg aaacctcacg atccgggagc
     2221 ggatcgaggc ggggctcggc tatacgccgg aagaccgcca gcgctacggc ctcgtcgccg
     2281 atttcacgat cgccgaaaac gcagtgctga aaaactacta tcacccccga tacacgggaa
     2341 ggttcggaat cctcaatgac caggcgatgc acgatgaggc ggaaaccctg atcgagcagt
     2401 tcgacattcg ggcgggggag ggttcccaca ccaaagcggg cagcctcagc ggaggaaacc
     2461 agcagaaagt catcgtggcc cgagagatct ccctgaggcc caaagtcctg atcgtcgccc
     2521 agccgacccg aggcttggat gtcggggcga tcgaatatat ccgaaagcgg atcatcgaag
     2581 agcgaagcag aggccaggcg attcttctgg tctcctttga gctcgatgag atcatgaacc
     2641 tctgcgaccg gatcgccaca atcagcaagg gaaccattgt agcggtgaac aggagcgatg
     2701 aagtgaccga acgcgatatc ggcatgatga tggccggctc ccggatgaag gagtccgtat
     2761 gaagaaaaag gatgcttgga ttcaaagtga cggggcggtg agtgtgctcg tcgtcctctt
     2821 ggggtttctg gtcgggacga ttctcgtcgc cctggtggga aagaacccgc tgaatatgta
     2881 caaggcgatc ctccaagcgc tcagcggata caatatcgac aatggaaaaa tgaacgtccg
     2941 ctatatcggc gagacgttga actattcggt tcccttcatt ctgtgtggtc tttcgatggg
     3001 attcgccaac cgcgtcggcc tgtttaacat cggaggggaa ggccaataca tcatggggat
     3061 gacggtcgcc cagatcatcg ccctgctggg accgcagatt cccgtgctgc actggcttct
     3121 cgccctcctg ggagcgatgc tcatcggggc ggtctgggga ggagtcgtcg gaatcctgaa
     3181 ggccaagtac gaggtcagcg aggtcgtctc gacgatcatg ctcaactacg tcgccctgta
     3241 tctctcgcgg atcatctgtc ttcagctgcc cggagcgacg acctatcgga cggcgagcta
     3301 tcccgagacc gccctgatca ccaacgtctt cttccagaag atcacgaaga actcgctctt
     3361 gaacaacggg atcttcttca tgattctcgc cgtcgtcgcc ttctggttca tcatggagaa
     3421 gaccagcctg ggctacggga tgcgggcgac cggcttcaac aaggaagccg cccgggcaag
     3481 cgggatcccc gtcgtccgtt cgatctccat ctcgatggcg atcagcggaa tcttcgccgg
     3541 cctcgccggc gggatcatcg ccctcggatc cttcaagtac ggaagggtga tcgccggcat
     3601 ggacaactac gggttcgacg ggatcgccgt cgccctggtc gggaactcca ccgcgctggg
     3661 aaccaccctt gccggcctgc tcttcggact cttgaagaac gcccaggcgc tgatgcaggc
     3721 caagcagatc cccaaggaga tcaccttcat catccaaggc ttgatcgtca tcttcatcgc
     3781 cttgcgctcg gcgctgagga tttataagga atacctcgat aagcgacggc ttcagaagga
     3841 ggtgggacta tgatgatctg gaacatgctt ccctccgtgc tgatgattgt ggctccgatt
     3901 ctcattacgg cgatcggggg gatgatcagc gagaagagcg gggtcgtgaa catcgccttg
     3961 gaaggattga tgatgatcgg ggcgatgagc gccgccgctg cccacgtctt gatggaaggg
     4021 tcggggatga accacaccct ttcgcttatt ctggcgatgg tgatcggaac ggtgatcggc
     4081 gggctcttct ccctccttca cgctgttgct gcgatcaacc tcaatgcgga tcagacgatc
     4141 agcgggacgg gaatcaacct gctggcaagc ggggtgacga tcttcgcttc gcagattctc
     4201 ttcaacgcgg accggacccg ggagttccgg atggggatgc agaccgatgc tctcgggttc
     4261 tatccgaccg cctatatcgc cttggtcgtg gtgctccttt cgtggttcgt cctctacaag
     4321 cttcccttcg ggatgcacct cagggcatgc ggcgaacacc cgggagccgc tgaaagcgtc
     4381 ggcatcaacg tgaagcggat ccgctacatc gccgtcctgg ccagcgggat gctcgccggc
     4441 cttgccggcg gatgcgtcgt gctgacccag acgatccagt atacctccaa tacgatcaac
     4501 gggcggggat tcatcgccct tgccgcggtc tccttcgggc gatggcgccc tgccggggtg
     4561 gccggagccg ccctcctctt cggaaccgcc caggcgttcg cgatcatcgc gaacaacata
     4621 cccgccctga gggtgctgcc caccgaggtc ttcaacatcc tgccctacgt ggtaaccctc
     4681 gttgccctcg tcctcttcag cgggaagaac tatgctcccg ccgctgcggg aaaaccctat
     4741 gaaaaggggg ccagctgatg agcgttcaca tcgcagcgaa aaaagtcgct tccgtcgtgc
     4801 tggccccggg ggatccgctt cgggcgaagc tggtcgccga gcgctttctc gaaaaaagca
     4861 gactgattac cgatatccga aacatcctcg ggtacaccgg tatctataaa ggggtggaga
     4921 tctccgtcat cgcgacgggg atgggcggtc cttcgatcgg catctacagc tacgagctgt
     4981 tcaacgagta tgaggtcgag acgatcatcc gaatcggcac ctgcggcggt ttccaaaagg
     5041 agattcttcc cggggatcgg atccttgctc tcacagcatc aacggacagc gcctgggccc
     5101 atcagtacaa cctgaaaggc accctcagtc ccgcctgcga tccggatctg ctcctcacgg
     5161 ctgcgcttct tgatccaagc gcgcatgtgg ggatggtctt ctccagcgat ctcttctcct
     5221 cctataacgc cctcggcgaa gagagctgga aagcatgggc ggcgatgggc gcacttgctc
     5281 aggatatgga gacatatgcc ctctatgcga ccgccgccca tgcgaacaag cgggctcttt
     5341 ccatgctcac gatgaccgac tgcctcgcaa cggggaacgc gcttcatgat gacgagcggc
     5401 aaagcgccct ctatccgatg atcgagctcg ccctcgaggt agcctatagg gaactatgaa
     5461 gatgaacttt ctcgacatca tgcgccatcg cttcgcctgc aaactctacg atgtggacaa
     5521 acccctgagt caagaggaga tcgatgaaat tctggagtac ggccgactga ccccgacctc
     5581 cttcggcctt gagatgtggc atttccatgc ggtcgtttca aaggagaagc tcaaggaact
     5641 ctataccgcc tgctatgaac aggaaggggt tcggaccagt ggaatgacgg tggcgatcct
     5701 ggtcaaaacc ccgaaacacc tcgatcccga cggatcgttc gtgtatgaac gggggatccg
     5761 cttcccgagc gatatcgact ggttcatcga ggattaccga ccctaccatg aatttttaaa
     5821 agacaatcat cggctggaag ggtggctccg aagtcagggg tatcttgcga tcgccaacat
     5881 gatgaccgga gcccgatcac tcggaatcca aagctgcgcg atcgaggggt ttgacgagga
     5941 gggggtgctc agggtcttgg gaatcgaccc cgaggagtgg ctcgtttctc ttctttgcac
     6001 cttcggccat gccgccgaag aggagcgacc gaagattcga gtacccttgg atgaattggt
     6061 tacctgtcat taaggaaggt ttctcaagga acgttccacc atctcccaga accaggggcg
     6121 gtcgatatcg gtggggatga tcactccgcc ttcaccgtcg atcgcttccc tgctcgtcat
     6181 gccgtaggag ggggaggcgg aatcgatatc gacgacaacc cgccgatctt cgacagcgaa
     6241 cctgtttcgg tcgatgacat acgccacgca gagcggatca tgcatctgcg gccagccgaa
     6301 cttcttggct tcgtagttcg ccatgtaggt gtccatgcat tggctgaaga cctccgccga
     6361 ccgtccctcg accgttctca ggtacttcaa tcgctccctc gagagcgtca cggtccgggt
     6421 gatatccagg gtaagcatga ccagcggcgc tccgctggaa aagacgatag cggctgcttc
     6481 cgggtcggca tacgcgttga attcagcatt ttcggtgacg tttccgccgc tgaacgaccc
     6541 gctcatgacg acgatctcct tcaccagcga gggaagggcg ggttctcttc gcaaggcgtg
     6601 ggcaagatcg gtcaacggac cgacgctgat gatcgtgatc tcccctggat gtcgtttgac
     6661 gaggcggatc atggcggaga ccccgtcctc ttcttgaagc tcttgaatga cccgttttcc
     6721 gaagaccggt ccatccagcc ccgactcgcc gtggaagtcg cccgcttcga ccggctggcg
     6781 gatcagcggc ttggcggaac cggcgaagac cggacacgaa aggccggtgg tttcgatcac
     6841 gttgagggtg ttttcaacgg tcttgtcaag accgacattg ccggcggtgg cgatgaccgc
     6901 gacgatctca agctcttctg cgccggcggc caaaaacagc gccgccgcat catccaatcc
     6961 ggtatcgaca tccaagatca ctttctgttt catggatcac atcctatcaa ctctttttgc
     7021 tcaacgcaat gaatcttttg tggtatcatg ggcgcatgag aatcattcaa accgccgtgg
     7081 gaccgaagcg ggttccgttg accggctatc tgcaggatat gacgatggac ggaggaatcc
     7141 ggaacattcg ccccacgatc atcgtctgcc ccgggggcgg atatacg
//
LOCUS       DAOSLI010000070         7097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9624_length_7097_cov_26.921045, whole genome shotgun sequence.
ACCESSION   DAOSLI010000070 DAOSLI010000000
VERSION     DAOSLI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9624_length_7097_cov_26.921045"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..247
                     /locus_tag="PLF67_08200"
     CDS             80..247
                     /locus_tag="PLF67_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE90028.1"
                     /translation="MAYKITDDCIACGTCLSECPTGAISEGDIYVIDADLCIDCGVCA
                     DACPTEAIIPE"
     gene            314..1507
                     /gene="xseA"
                     /locus_tag="PLF67_08205"
     CDS             314..1507
                     /gene="xseA"
                     /locus_tag="PLF67_08205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOE90029.1"
                     /translation="MDVPTITVTELTRSIKQVLEEGFPFFSVTGEISNFRPASSGHWF
                     FTLKDEGAQIGCVIFRSNAWKVTFTPQDGDKVTITGSLDLYPPRGTYQIKCETIVKTG
                     FGNLLALLEERKTRYQQKGYFDRKRPLPKTPRRLGIITSATGAALQDVLSVLGRRAPS
                     LEILIFPSPVQGAEAAQSIAFRIEQANLLSDCDVLLITRGGGSIEDLLPFSEEAVLEA
                     IHASEIPTVSAIGHEIDWALSDFVADLRAPTPSAAAELISKGIYDSKTELVRIRQRLI
                     AAIEYRLLRAEAAVGKVSVPALRAYLLAQVDRRQFRLANAQLALSNGIDDQLARRRNK
                     LTLLSNQLSALSPLAVLERGFALVSTKGGAVLRSSKPVHDGDRLKIRWIDGERSVRVE
                     EQDEL"
     gene            1497..1715
                     /gene="xseB"
                     /locus_tag="PLF67_08210"
     CDS             1497..1715
                     /gene="xseB"
                     /locus_tag="PLF67_08210"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOE90030.1"
                     /translation="MNFEEKIAKIETITKKLQDEHTSLEDSIALFEEGVTLAKELEQA
                     LEEAKGKVEQVVGESLTSMEVVEFDDEQ"
     gene            1705..2568
                     /gene="purU"
                     /locus_tag="PLF67_08215"
     CDS             1705..2568
                     /gene="purU"
                     /locus_tag="PLF67_08215"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOE90031.1"
                     /translation="MNNKIVFLIQSEDRKGILSATTTFFSTLGFNILHCQQHTDAREG
                     RYFMRIELNLSDLPSTRSDLEKRFARFAEEQRLTWRCHWGDYRSRIAILVSKTSHCLY
                     DLLFHVSEGDLFCDIPLIISNHPDLESVANLFRIPYYHLPVTKDTKEEQEAEILRLLE
                     RFDIDLVVLARYMQILTPSFTDRWYGRIINIHHAFLPAFQGANPYQQAYERGVKMIGA
                     TAHYASEELDRGPIIEQDVVRVSHELDPSGLRNLGKDVERRVLTKAVSAHLESRVIIY
                     KNRTIVFGPIV"
     gene            complement(2565..3668)
                     /locus_tag="PLF67_08220"
     CDS             complement(2565..3668)
                     /locus_tag="PLF67_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOE90032.1"
                     /translation="MADQRQIRLAKLLVEYSTKVQKGEKVLINAVDVPVPMVEELIKA
                     VYEAGGFPIVNLWNQRIERAIVEGASEASLKAWADVDMYRMNRMDVFIGIRGVANVRE
                     LATIPNQNNLASLHYSKPVHMDIRVPKTRWVVLRYPTEVMALQANMGTREFEDFFYSV
                     CTDVDYAKMAKAMAEAKKYLDTVDKVRIVAPGTDLRFSVKNIPWVPCAGEMNIPDGEI
                     YTAPVKDSVNGTITYNTPSTNGGHLFRDIRFTFKDGKIIEAHADDDRLLNEILDRDEG
                     ARYVGEFALGCNPQILHPMDNTLFDEKISGSFHFTPGNAYEDADNGNRSSVHWDLIQI
                     QTPEFGGGEIYFDGVLVRKDGVFVDERLAMLNL"
     gene            complement(3675..4391)
                     /locus_tag="PLF67_08225"
     CDS             complement(3675..4391)
                     /locus_tag="PLF67_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE90033.1"
                     /translation="MLDKTGVRIAYEDTHILVVDKPAGLATVPLKEDPPDKPTLLGVV
                     ASEYPETLKPFGRNTWEGGVLHRLDTPTSGLVIIARTQYGFDALTAASRAELVTKEYW
                     LGSHLRAKPPKGFPAYPYEDPAFAGGKEVVIGSLFRPWGEKRREVRPVLSESPRHLLE
                     KTSGVWYMTKVVAQGSGVFRARITSGFRHQVRAHLAWSGHPIDGDDRYGGKPDEKLLL
                     RATAVEFPFDGRIREVRVKE"
     gene            4452..5381
                     /locus_tag="PLF67_08230"
     CDS             4452..5381
                     /locus_tag="PLF67_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE90034.1"
                     /translation="MSEIPINTLTSSSVILELILRLKIKDVMSTNVITATKETPLRKI
                     QEIMREERITGLPIVMGKRLVGLVSMDDIIKALDQGYIEEPAQNHMSQDLIVLDEEMP
                     VSFAINYFSRYRYHRFPVLNKYKHLVGMVTNRDITTVLLVEINKEVERLEKLAKVQTH
                     ATDMSGEVHTYPIIKNDFENAGSASTQIKKRLKEAKIDNAIIRRAAIASYELEMNMVV
                     HSDGGTLKAEYTPTTLTLTAQDRGPGISDIDGALTPGWSTASEWIRSLGFGAGMGLPN
                     VKNVVDTFFIESDVRGTTVSCIINLIPKEEDHS"
     gene            5378..5704
                     /locus_tag="PLF67_08235"
     CDS             5378..5704
                     /locus_tag="PLF67_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur binding hydrogenase"
                     /protein_id="HOE90035.1"
                     /translation="MTVKDLSQVEGLKAHHVADGDQAVKDIYCGDLLSDVMANAQEDS
                     ALLTIQAHKNTVAVASLLGLGVIVLCNGRTPPSDMIEAAKSEDISIFSSSANHYKTAL
                     AITPFL"
     gene            5708..6412
                     /locus_tag="PLF67_08240"
     CDS             5708..6412
                     /locus_tag="PLF67_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOE90036.1"
                     /translation="MGLYRIDLHNHSSLSPCAEDDFSPFLLAIEAKEKGIDILGLSDH
                     NSTDNLKAFAEACKIVDLIPFFGIEVTTIEEVHVLVSFVHLAPALAFGSWVNDHLPYY
                     PNNEKLFGRQLIYDEEGNQTAEVEQMLATATTLGFEEVIDKALKEGALAIPAHIDRGS
                     NSVLSNLGFLPELPYSAIEVVTPRSEFEELGYPVLTGSDAHRFEEVGIRSCLMELSDR
                     SAKALIAALNGRRCGE"
     gene            complement(6375..7082)
                     /locus_tag="PLF67_08245"
     CDS             complement(6375..7082)
                     /locus_tag="PLF67_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOE90037.1"
                     /translation="MMQEQKMMHTAIARERTILKNVYLWMTAGLALSGLVAWLVAGSP
                     ALLRAMVGNPLSFLILVIAQFSLVFYLTARLDRMSASSAIGAFLAYAALNGAMLSTIF
                     IVYTQALIARAFFTTALMFAGMSLYAMTTTRDLHRFGTYLVMGLWGLIIASVLNMLFR
                     SSGLDFLISIAGVVIFLGLTAWDTQKIVAMNHAYGDSDDYTLVTRLSIIGALTLYLDF
                     INLFLFILRIFGRSERR"
BASE COUNT         1681 a         1985 c         1893 g         1538 t
ORIGIN      
        1 tctttttttc tacctcttgt tgactagaac caaagggggg tatatagttt tgacaacgat
       61 accaatcaag gagctttcca tggcttacaa aatcacggat gactgcatag cttgcggaac
      121 atgtctctcg gagtgcccga ccggggcgat cagcgaaggc gatatctatg tgatcgacgc
      181 cgacctctgc atcgactgcg gtgtctgtgc agatgcctgc cccactgaag cgatcattcc
      241 cgagtaagga gtattgcttt gcgattgaga cagcttatcg gggctgtctc ttttttttca
      301 aaggattgtt gacgtggatg ttcccaccat tacggttacc gaattgaccc gctcgattaa
      361 acaggttctt gaggaaggat tccccttctt ttcggtcacc ggagaaatct caaactttcg
      421 ccccgcttcc agcggtcact ggttctttac cctcaaggac gagggagctc agatcggttg
      481 cgtaatcttt cgctcaaacg cgtggaaagt aaccttcacc cctcaagacg gggacaaggt
      541 gacgattaca ggatcgttgg atctctatcc tcctcgagga acctatcaga tcaagtgcga
      601 gacgatcgta aaaaccggct tcggaaacct gctcgctctc ctcgaagagc ggaaaacgcg
      661 ctatcagcaa aaagggtatt ttgaccgcaa gcgcccgttg ccgaaaactc cgagaaggct
      721 gggcatcatc acaagcgcca ccggagcggc gttgcaggac gtgctctcgg tccttgggag
      781 gcgagccccc tccttggaga tcttgatctt cccttccccg gttcaaggcg ccgaagcggc
      841 tcaatcgatc gcatttcgga tcgaacaggc gaatctgctt tccgactgcg acgtgctcct
      901 gatcacccgc ggcggcggct cgatcgaaga tctcctgccg ttcagtgaag aggcggttct
      961 ggaagcgatt catgcatccg agattcccac cgtcagtgca atcggccatg agatcgactg
     1021 ggctctcagc gactttgtcg ccgacctgcg cgctccgact ccgtctgcag cggccgagtt
     1081 gatcagcaag gggatctacg attccaagac cgagcttgtt cgaatcaggc aacgcctgat
     1141 cgccgcaatc gaataccggc tcctgcgggc tgaagcggcg gttgggaaag tcagcgttcc
     1201 tgcactcagg gcatatctgc ttgctcaggt cgaccgtagg caattccgtt tggcaaacgc
     1261 ccagctggcc ctttccaacg gtattgacga tcagcttgcc cgaagaagaa acaaactgac
     1321 gctgctgtcc aatcaattaa gcgccttaag tccgcttgcc gtcctcgagc ggggattcgc
     1381 ccttgtctct acaaaagggg gggcggttct tcgaagcagc aagccggtgc acgatgggga
     1441 caggctgaag attcgttgga tcgatggaga acgatcggtc cgagtggagg aacaggatga
     1501 actttgaaga aaagattgca aaaatcgaaa cgattaccaa aaaactgcag gatgagcaca
     1561 cctcgctcga agattcaatc gctctctttg aagaaggggt aacactggca aaagaactcg
     1621 agcaagcgtt ggaagaagca aaaggaaaag tggaacaagt ggtcggtgaa agcttgacct
     1681 cgatggaagt ggtggaattc gacgatgaac aataagatcg tctttctgat ccaaagcgag
     1741 gatcggaagg ggatcctcag cgcgaccacg accttcttca gcaccttggg cttcaacatc
     1801 ctccactgtc agcagcatac agatgcccgg gaagggcgct acttcatgcg catcgagctg
     1861 aatctctccg atctgccgtc gacgaggagc gatctggaaa aacggttcgc ccgctttgcc
     1921 gaggagcaac ggctcacctg gaggtgccac tggggcgact accggagccg gatcgcgatt
     1981 ctcgtcagca agaccagtca ctgcctgtat gacctgctct tccatgtcag cgagggggat
     2041 ctcttctgcg acatcccttt gatcatcagc aatcaccccg atctggaaag cgtggcgaac
     2101 ctgtttcgga tcccctatta ccatctgccc gtgacgaagg ataccaagga ggagcaggag
     2161 gcggagatcc ttcggcttct ggagcgcttc gacatcgatc tggtcgttct ggcccgctac
     2221 atgcagatcc tcaccccctc cttcaccgat cggtggtatg gaaggatcat caacatccac
     2281 catgcgttcc tccccgcatt ccaaggggcg aacccctatc agcaggcgta tgaacggggg
     2341 gtgaagatga tcggggcgac cgcccactat gcgagcgagg agctcgaccg ggggccgatc
     2401 atcgaacagg acgtggtccg ggtcagccac gagctcgacc cttcaggctt gaggaacctc
     2461 ggcaaggacg tggaacggcg cgtgctcacc aaggcggtga gcgcgcatct cgaaagccgg
     2521 gtcatcatct acaagaatcg gacgatcgtc ttcggcccga tcgtttaaag gttcaacatc
     2581 gcaaggcgct catcgacgaa gaccccgtcc ttgcggacaa gaactccgtc gaagtagatc
     2641 tctccacccc caaactcggg ggtctggatc tgaatcaagt cccagtgcac cgaagagcgg
     2701 ttgccgttgt cggcatcctc gtaggcgttg cccggggtga agtggaacga tccggaaatc
     2761 ttttcatcaa aaagcgtgtt gtccatcgga tggaggatct gcgggttgca gccgagggcg
     2821 aactctccaa cgtagcgcgc tccttcatca cggtcaagaa tctcattaag caggcgatcg
     2881 tcatcggcgt gcgcttcgat gatctttcca tctttgaagg taaatcggat atccctgaag
     2941 agatgtccgc cattggtcga cggcgtgttg taggtgattg ttccgttgac cgagtctttg
     3001 accggggcgg tgtagatctc cccgtcggga atgttcattt cgcctgcgca cggcacccaa
     3061 ggaatattct ttacgctgaa gcgcaagtcc gtgcccggag ccacgatcct aaccttgtcg
     3121 acggtatcga ggtacttctt cgcctccgcc atcgctttcg ccatctttgc atagtcgacg
     3181 tcggtgcata cgctatagaa aaaatcctca aactcccgcg ttcccatgtt cgcctgaaga
     3241 gccatgactt cggtgggata gcggagtacg acccatcggg tcttgggaac ccggatatcc
     3301 atgtggaccg gtttggagta atggagggaa gcaagattgt tctggttggg gatcgtggca
     3361 agttcgcgca cgttggcgac gccgcgaatt ccgatgaaca cgtccatccg gttcatccga
     3421 tacatatcga catccgccca tgccttgagc gacgcttcgc ttgcgccttc gacgatcgcc
     3481 cgttcgattc gctggttcca gaggttgacg atcggaaacc cgcccgcttc atataccgcc
     3541 ttgatcagct cttctaccat cggaacggga acatccaccg cgttgatcaa aaccttctcg
     3601 cctttttgca ctttggtcga atactcgacc aacaacttgg ctaatcggat ctgtcgttga
     3661 tctgccatct cttcttactc ctttactcgt acttctctta ttcttccatc aaaggggaat
     3721 tcaaccgcgg tcgctcgaag aagaagcttc tcatccggtt tgcccccgta tcgatcatca
     3781 ccgtcgatcg gatgtccgct ccacgcaaga tgtgcccgca cctggtggcg gaagccgctg
     3841 gttatccggg ctcgaaacac tcccgaacct tgagcaacga ctttcgtcat gtaccacact
     3901 ccgctggttt tctccaagag gtggcgcggc gattcgctga gaaccgggcg cacttcccgt
     3961 cttttttctc cccaaggacg aaacagcgaa ccgatgacga cctcttttcc tccggcaaac
     4021 gccggatcct catagggata ggcaggaaag cctttcggcg gtttggccct tagatgtgaa
     4081 ccgagccaat actctttcgt aaccaattcg gctcgggaag cggctgtgag ggcgtcaaac
     4141 ccatattggg tccgggcgat gatgaccagc ccgctggtgg gtgtatccaa tcgatggagc
     4201 actcctcctt cccaagtgtt tcggccgaac ggcttgagag tttcaggata ctctgaggcg
     4261 acgaccccaa ggagggtcgg tttgtcggga ggatcttcct tcaaaggtac ggtggccaaa
     4321 cccgccggct tgtccacaac aagaatatgg gtatcttcat aggcgattcg cacgcccgtt
     4381 ttgtcaagca tggcagaatt aattatatca aattacccac taaagtacac agatgatgta
     4441 tactatttcc aatgtcagag ataccgatta acaccctaac ctcctccagt gtgattttag
     4501 agctgattct tcgcctcaaa atcaaggatg tgatgtcgac aaacgtcatc actgcgacga
     4561 aagaaacgcc ccttcggaaa atccaggaga ttatgcggga agagcggatc accggcctgc
     4621 cgatcgtgat gggcaaacgt ctcgtcggac ttgtttcgat ggatgacatc atcaaggcgc
     4681 tcgatcaggg gtacatcgaa gagcctgcgc aaaaccatat gagtcaggac ctcatcgtgc
     4741 tggatgaaga gatgccggtc agttttgcaa ttaactattt ctcccgttat cgctatcacc
     4801 gttttcccgt tctcaacaaa tacaagcacc ttgtcggaat ggtcaccaac cgggacatca
     4861 ccaccgtcct tctggttgaa atcaacaaag aggtcgaacg gctggaaaag cttgcgaaag
     4921 ttcagaccca tgccaccgat atgagcgggg aagtgcacac ctatccgatc atcaagaatg
     4981 actttgaaaa tgcgggctcc gcttccaccc agatcaaaaa acgcctgaag gaagccaaaa
     5041 tcgacaatgc gatcattcgc cgggcggcga tcgccagtta tgagctggag atgaacatgg
     5101 tggtccactc cgacggagga acgctgaaag cggagtacac tcccacgacc ctgacgttga
     5161 ccgcccaaga ccgcgggccg gggatcagcg atatcgatgg ggcgctcacc cccggttggt
     5221 caactgccag cgaatggatc cgatccctcg gtttcggtgc gggaatggga ctgcctaacg
     5281 tgaaaaatgt cgtcgatacg ttttttattg aaagtgatgt tcgcggaaca acggtttcct
     5341 gcatcatcaa tttaatcccg aaggaggaag accattcatg accgtcaagg atctcagcca
     5401 agtagaaggg ttgaaagccc accatgtcgc cgatggagat caagcagtga aggatatcta
     5461 ttgcggagat ctgctcagtg atgtgatggc caatgcccag gaagacagcg ccctgctcac
     5521 gattcaggca cataaaaaca cggtagcggt cgcttccctc cttggcctcg gcgtcatcgt
     5581 cctctgcaac gggcgaactc ccccctcgga tatgatcgaa gcggcaaaaa gcgaagacat
     5641 ttcaatcttc tcaagttccg ccaaccacta caagaccgcg ttggcaatta cgccatttct
     5701 ctaagcgatg ggtctgtacc ggatcgatct gcacaaccac tcatcccttt cgccgtgcgc
     5761 cgaggatgat ttctccccct ttctgctggc gatcgaagcg aaagagaaag gaattgacat
     5821 cctcggcctc agcgaccata acagcaccga taacctcaaa gcgttcgccg aagcctgcaa
     5881 aatcgtcgat ctcatcccct tcttcggcat cgaagtgacc accatcgaag aagtgcatgt
     5941 gctcgtctcc ttcgtacacc tcgccccagc cctcgccttc ggttcgtggg tcaacgatca
     6001 cctaccctac tatcccaaca atgaaaaact cttcggtcgg caactgatct atgatgaaga
     6061 gggtaatcag acggcggaag tggagcagat gcttgccacg gcgacgacct tgggctttga
     6121 agaggtgatc gacaaagcgc ttaaggaagg agcgctcgcc atccccgccc acatcgaccg
     6181 gggaagcaac agcgttctga gcaacctggg ctttctgccc gagctgccct attcggcaat
     6241 cgaagtggtg acgccgagaa gcgaattcga agagcttggc tatccggtcc ttaccggcag
     6301 cgatgcccac cgttttgaag aggtgggcat cagatcgtgc ttgatggaac tatcggatcg
     6361 ctcggccaaa gcccttatcg ccgctctgaa cggccgaaga tgcggagaat aaacaagaaa
     6421 aggttgatga aatccagata cagggtcaat gctccgatga tcgagagcct ggtgaccagt
     6481 gtgtaatcgt cgctgtcgcc ataggcgtgg ttcatcgcca cgatcttttg ggtgtcccag
     6541 gcggtcaggc cgaggaagat gaccactcct gcgatcgaga tgaggaaatc aaggccgctg
     6601 gacctgaaga gcatattcag tacgctggcg ataatgagcc cccacagccc catgaccaga
     6661 taggtgccga agcggtgcag atcccgggtg gtggtcatcg catagagact catcccggca
     6721 aacatcaaag ccgtggtgaa aaacgcccgg gcgatcaggg cctgcgtgta aacgatgaaa
     6781 atcgtgctca acatcgctcc gttcaacgcg gcatacgcca aaaacgctcc gattgcgctc
     6841 gaagcgctca ttcgatccag ccgggcggtc aaatagaaga ccagggaaaa ctgggcaatg
     6901 accaaaataa gaaagctcaa agggttcccg accatcgccc tgaggagcgc cgggctgccg
     6961 gcgacaagcc aggcgacaag cccactgaga gcaagaccgg cggtcatcca taggtataca
     7021 ttttttaata tcgtccgctc gcgggcgatc gcggtgtgca tcatcttctg ttcctgcatc
     7081 atccacctcc aatatac
//
LOCUS       DAOSLI010000071         7044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9713_length_7044_cov_46.118043, whole genome shotgun sequence.
ACCESSION   DAOSLI010000071 DAOSLI010000000
VERSION     DAOSLI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9713_length_7044_cov_46.118043"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="PLF67_08250"
     CDS             <1..1628
                     /locus_tag="PLF67_08250"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOE90038.1"
                     /translation="QETLVDLIINLTSIIHQKDEEIRQKDEENKRLKEARRLQLATQY
                     VPSTEQMGYLFEELELLDEVLKTEVEETLKVEVSAHVREVRKRSNACTAPSDTPVVDV
                     YHEKDAPATLVKDEITYDRVEDKIIDKLAIIPRRYVVERNHYPQYRARDVEAPKLIIG
                     LGGAANIGGSPSLVANTIVSKFDDHLPLYRQEEIFKREGIFLTRQKLANWVITYYEHL
                     LPFASFFKRQLYESNLISKDETSVQVLNVKGPDGKPSKNGFMYITVGETYDPDTRSTR
                     TLVNLDYIQGRSRDVLFEDINKYKYDGYLMSDGLAGYLAYPKEKHGVCWVHAVRKFKT
                     ILKENPKNLQAAKVVALVSKLFTIEQELRTHLHEGSLDPQGFLTLRREKSQSIIDSVY
                     EEVDGFRSDFTPNGAMGKAISYLDTYKPYLNVYLDVLEATPSTNNCERIAKAFATGRK
                     NWLFSETVDGADASAFFYSLVETAKFSKIDPLDYVEAICTYGIYAKSEDDWMSLLPDR
                     IDLSALYERRANLASAKADPTREKPYHFVGHTK"
     gene            complement(1621..1968)
                     /locus_tag="PLF67_08255"
     CDS             complement(1621..1968)
                     /locus_tag="PLF67_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90039.1"
                     /translation="MLRTYTHEQKLQYLKDGYEFVRSGSGTYKAYIAGIGVSRGLFYQ
                     WIKKHATEAGIPARGRRKSSNRSLVPIGKPKGQSPVMGSHFSVEFFEARINVSSEEDL
                     VTVLKAVKQASVI"
     gene            complement(2204..3364)
                     /locus_tag="PLF67_08260"
     CDS             complement(2204..3364)
                     /locus_tag="PLF67_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90040.1"
                     /translation="MSVTRFASVLLMFLLVPALLFGAITATYYAEPSLYLQVQPGPYT
                     SDTVIGAKLGYLEVTTDGEEIYSPAWGSTSENFLSAYLHGPMRLIPGGPLGQWPYEFH
                     IMSVAYPEGYPGPPIIITKVDYPYSPIIKNEPEQVKVSPFRVELYLVNTNSVNRNIKV
                     KPPELPASCFEPNESYTLTAFMGARFDLHYSFVVANQKGTKVNEMMNWWDGEPNSSGS
                     YVSVGGNTGSNSIAVTSGLDPGNPEVPYGDPSTMVNFSVFLSEQSTTFDLSEAVGTNR
                     KDVNTMTITVSNGIAGADYSQKVIFTDLTPVSTSAEFRMLPEESGPNPIPFKLYFDST
                     EVVKGEAITWSSLQNGSSNTKTIRIGGIDQNIIDSLASGTYSDTINVEIRNP"
     gene            complement(3746..4312)
                     /locus_tag="PLF67_08265"
     CDS             complement(3746..4312)
                     /locus_tag="PLF67_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90041.1"
                     /translation="MQGQTGNANPSNGTYAIVNGNGGPDSTPIVESTAYTDPDNPNSP
                     VMTYVDPSQIVSYSVFLSEQTKTFNLSEAVGTNRKDVNTMTITVSNGIAGADYSQKVI
                     FTDLTPVSTSAEFRMLPEESGPNPIPFKLYFDSTEVVKGEAITWSSLQNGSSNTKTIR
                     IGGIDQNVIDSLASGTYSDTINVEIQNL"
     gene            complement(4984..>5223)
                     /locus_tag="PLF67_08270"
     CDS             complement(4984..>5223)
                     /locus_tag="PLF67_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90042.1"
                     /translation="LKIHSFRPFMQGETLIGGRVPDNFEAPMMNINKIIDDKMHKHVD
                     NVCTDGVIGDRKKLGHRIRDFRERLQIKLITAVQY"
     assembly_gap    5224..5323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5405..>7044
                     /locus_tag="PLF67_08275"
     CDS             5405..>7044
                     /locus_tag="PLF67_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOE90043.1"
                     /translation="MFTIDYAKTPVTDFYGINCFTEAEMGKYISDPIIRQLKEVQRGQ
                     REMNEELADYIASAMKQWALNRGATHFCHWFQPLTGLTAEKHDSFISPTKGGKVLLEF
                     SGKELIKGEGDASSFPNGGLRETFEARGYTAWDTSSPAFLKEVHGIKTLYIPTAFFSY
                     KGEALDKKVPLLRSNQAIEKQALRVLRALGNTTAKRITVNIGPEQEYFLVDRKYYEQR
                     TDLRLAGRTVIGSLGAKSQEQNDHYYGTIGDRVAVFMSELNYELWKLGISSKTQHKES
                     APNQFEIAVVYDAANLSTDHNQLLMDVLQMVALRHGMVALLHEKPFAGVNGSGKHDNW
                     SLATEDGTNLYAPGTNVEDNVQFLIFLTSFIKAMDEYAPLIRSGAATAGNDLRLGSSE
                     APPAVISIYLGDQLTTILETLSKEGNCEKRVREVVKMGTTILPELPKDLTDRNRTSPI
                     AFTGNKFEYRMVGSSQSMAGPNIFINAAVAQVLGEAADRLEAATDKEAESHKIISDFY
                     QNHKRIIFNGNGYSEEWAKEAKKRGLPHYRDTVSALPQ"
BASE COUNT         1764 a         1791 c         1686 g         1703 t
ORIGIN      
        1 gccaagaaac gcttgttgat ttaatcatca accttacctc catcatccat cagaaagatg
       61 aagagattcg ccaaaaggat gaagagaata aaagactgaa agaggcaagg cgtcttcaat
      121 tggcaacgca gtatgttcct tccaccgaac agatgggata tctgtttgaa gagcttgaac
      181 tgcttgatga ggttcttaaa actgaagtcg aagagaccct caaagtcgaa gtaagtgctc
      241 atgtcaggga agtgagaaag cgctccaatg catgtaccgc tccttctgat actccggtcg
      301 tcgatgtata tcatgaaaag gatgctccgg ctactcttgt caaagatgaa atcacctatg
      361 accgtgttga agacaagatc atcgacaagc ttgccatcat cccccgccgc tatgtggtgg
      421 aaagaaacca ttaccctcaa taccgggccc gtgatgtcga agctcccaaa ctgattatcg
      481 gtcttggagg ggctgcgaac atcggaggtt ctccaagcct ggtagccaat acgatcgtaa
      541 gcaagttcga tgaccacctt cctttgtatc gtcaggaaga gatcttcaag agagaaggaa
      601 tctttctgac caggcagaag cttgctaact gggtcatcac ctactatgag catctgcttc
      661 ccttcgcttc cttcttcaaa cgtcaactgt atgagtccaa tctcatcagc aaggatgaaa
      721 caagcgttca ggtgttgaat gtgaaaggcc ctgacggaaa gcccagcaag aacggcttca
      781 tgtatatcac cgtaggagag acgtatgacc cggatacaag aagcaccagg acattggtga
      841 atctcgatta catccaaggt cgttcccggg atgtcttgtt tgaagacatc aacaagtaca
      901 agtacgatgg ctatctgatg agtgatggat tggcagggta tcttgcatat ccgaaagaga
      961 agcacggagt atgctgggtt catgcggtac ggaagttcaa gacgattctc aaggagaacc
     1021 ccaagaacct tcaggctgcc aaggttgtag ctttggtatc caagctcttt accatcgaac
     1081 aggaattgag gactcatctt catgaagggt ctcttgaccc acaggggttt cttactctca
     1141 gaagagagaa gagccaaagc atcatcgatt cggtctatga agaagtggat ggcttcagat
     1201 ccgacttcac tcccaacggt gccatgggta aagcgatctc ctatctggac acctataagc
     1261 catacctgaa cgtatacctc gatgtcttgg aagcgacacc gagtacgaac aactgtgaac
     1321 ggatcgccaa ggcgtttgcg acaggaagaa agaactggct gttcagtgag acggtggatg
     1381 gtgctgatgc atcggccttc ttctattccc tggtagagac cgccaagttc tccaagatcg
     1441 atccccttga ttatgtggaa gcgatctgca cctatggcat ctatgcaaag agtgaagatg
     1501 attggatgag tctgcttccg gatcggatcg acctgagtgc cctgtatgaa agaagagcga
     1561 acttggcttc cgctaaagcc gaccctaccc gggaaaagcc ctatcacttt gtcgggcata
     1621 ctaaataacg gaagcttgct tcacagcttt cagaaccgtt accagatctt cttcggaaga
     1681 cacgttgatt cgagcttcaa agaattcaac gctgaagtga ctacccataa ccggtgattg
     1741 cccctttggc ttcccgattg gaaccaatga ccgatttgat gatttccttc tccctctagc
     1801 cggtattccg gcttcagtag cgtgcttttt aatccattga taaaacaatc ctctggaaac
     1861 cccaatccct gcaatgtatg ccttgtatgt accgctaccg gaacgaacga attcatagcc
     1921 gtccttcaga tactgaagct tctgttcgtg ggtatatgtt ctgagcattt gtctcctccc
     1981 aatgatacag atccatgata tctggaagag ggatggtcag aaatggggat ttattgatcg
     2041 cttacatttg accccatctt atgacttatc tttccatcga tctccgacac cctctcgcct
     2101 tgtgcaaaga gtcgatgcat ccggccatgt tcagcgcccc ggctcgggta tggccatctt
     2161 tggagtcttg gcaagatcct tcagagaata atgctgctct tggttatgga ttcctgatct
     2221 ccacgttgat cgtatcggag tatgttccgc tggcaagtga atcgattatg ttttggtcga
     2281 ttccgccgat ccggatcgtt ttcgtgttgc tggaaccgtt ttggaggctg ctccacgtga
     2341 tggcctctcc ctttaccacc tccgtcgaat caaaatagag cttgaaggga atcgggttgg
     2401 gtccgctttc ctcgggaagc atcctgaatt cggcggatgt ggatacgggc gtcaaatcgg
     2461 tgaagattac cttttgggag tagtcggcac cagctattcc gttggagacg gtgatcgtca
     2521 tcgtgttcac atcttttcgg ttcgtaccga ccgcttcgga cagatcgaac gtggtcgatt
     2581 gttcgctcag gaacacgctg aagttcacca tggtcgaagg atcgccgtac ggcacttccg
     2641 ggtttccggg atccaaaccg gaggtgacag caatgctgtt tgacccggta ttaccgccga
     2701 cgcttacgta tgatccgctc gaattgggtt caccatccca ccaattcatc atctcgttga
     2761 cttttgttcc cttctggttt gctacgacga agctgtaatg taggtcaaac cttgccccca
     2821 taaaagctgt caaggtatat gactcattcg gttcaaaaca gcttgccggt agttccgggg
     2881 gtttaacttt tatgttacgg ttcactgaat tcgtattgac gagatacagc tccacccgaa
     2941 acggactgac cttcacttgt tccggctcgt ttttgataat cggagagtaa ggatagtcga
     3001 ccttggtgat gatgatcggc ggtccggggt atccttcagg atatgcaacg ctcatgatgt
     3061 ggaattcata cggccattga ccaagtggac cgccgggaat taatctcatc ggtccatgta
     3121 aataagcaga caaaaaattt tcgctggtgg aaccccatgc cggtgaatag atctcctcac
     3181 catcggtcgt cacctccaag tatcccagtt tcgccccgat gaccgtatcg ctcgtgtagg
     3241 gaccgggttg aacctgaaga taaagagacg gttcagcata atatgtggcg gtgatcgctc
     3301 cgaagaggag tgcgggtaca agcaagaaca tcaagagaac agatgcaaat ctcgtaacac
     3361 tcataccatc ccctccgtat tctatcgcaa agccctccaa ccaactcttt gttttatagt
     3421 ataccagaac atcgaacaaa gcaatttttt aatgtgtact atcgtaaata taaagattta
     3481 ttcagatttt ctttcattaa tctcaaaaca tcaccatctt gtgttccaac ttttctttcc
     3541 cgttgctgcc ttcatccaca gaagatgagc agcatgaata cagccggaag ccagcacatg
     3601 gtgattcgaa ggaatgatcc tgaaacaagt cggtattccg tgcacgatga gcgggtggtt
     3661 gtactgcgtc agagactcta ctttatagtt atgttttgtc tatccataag acactattac
     3721 ttccagttga acgtgcgtgt cttgatcaga ggttctgaat ctccacgttg atcgtgtcgg
     3781 aatacgttcc gctggcaagt gaatcgatta cgttctggtc gattcctccg atccgaatcg
     3841 ttttcgtgtt gctggaaccg ttttggaggc tgctccacgt gatggcctct ccctttacca
     3901 cctccgtcga atcaaaatag agcttgaagg gaatcgggtt gggtccgctt tcctcgggaa
     3961 gcatcctgaa ttcggcggat gtggatacgg gcgtcaaatc ggtgaagatt accttttggg
     4021 agtagtcggc accagctatt ccgttggaga cggtgatcgt catcgtgttc acatcttttc
     4081 ggttcgtacc gaccgcttcg gaaagattga acgtcttcgt ttgttcacta aggaagacgc
     4141 tgtagctgac aatttgtgaa ggatcgacgt acgtcatcac cggagaattg gggttatcgg
     4201 gatcggtata cgcggtagac tcgacgattg gggtgctatc cggaccgcca ttaccgttga
     4261 cgatcgcata tgttccgttc gaggggttgg cgttgccagt ctgtccttgc atcatgctgt
     4321 ttgcattcct atcaggtctg ttagcggtga cgaaactgaa ctccggtctg aagctctcca
     4381 gcgtatacgc agcgttcatt tgaaaatcac ttgccgagca ggtcgtgctt ggattattag
     4441 tattattatt tgtgtcagcc gtgttcagca gccaaagctc cacgataaac ggatcggcgc
     4501 tcaccgtaac ccaattttga ggtaagctga caatcgaaag aaaagccccg gaaaacactt
     4561 tgatggttgg cggttgggac tctccattag gatacgcgac actcatcagg tagaacatag
     4621 cctcattgga tccattcgac atcggacctt taaccttcac cgttcctgac gctacctcgg
     4681 tcgtgttgcc ccataccggt gtatatatcg gaccgccgtc caccctgaga gttcccagtt
     4741 ttatcccgat gaccgtatta ctcgtatagg gacctggtgc agtcttaagt tccaagggca
     4801 cagcagtata ggttacggtg atcgccccaa acaggaggga tggaatagac gaaagcatca
     4861 agagaacata tgcaaatctt gtcaagctca tccgatcatt ccttcttacc atacagcccc
     4921 ctgtcgagtc tccatcagac tatataaaaa catcgaattg tgcaaatcat gacatgggta
     4981 ttatcaatat tgcacagcag ttatcagttt tatctgtaat ctttctctaa aatcacgtat
     5041 cctatgtcct aacttttttc tatcaccgat taccccatcg gtgcatacat tatccacatg
     5101 tttatgcatt ttgtcatcaa ttattttatt aatattcatc atgggtgcct cgaaattatc
     5161 cgggacccta cccccgatta gcgtctcacc ttgcataaag ggcctaaaac tgtgtatctt
     5221 caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaccccc gattagcgtc
     5341 tcaccttgca taaagggcct aaaactgtgt atcttcaagc cagagttcaa aaaggatgaa
     5401 agccatgttc acgatcgact acgcaaagac gccggtaacc gatttttacg gaatcaactg
     5461 cttcaccgaa gcggagatgg gcaaatacat cagcgacccc atcattcgcc aactgaagga
     5521 agtgcagcgc ggtcaacgtg agatgaatga agagcttgcc gattacatcg ccagcgcaat
     5581 gaaacagtgg gccctgaacc ggggggcgac ccatttctgc cactggttcc agccgctgac
     5641 cggcctgacc gccgagaaac acgattcctt catcagcccc accaaagggg gaaaggttct
     5701 tctcgagttc agcggaaaag agctgatcaa aggtgaaggc gacgcttcat cctttcccaa
     5761 cggaggcctt cgggaaacat ttgaagcccg gggctacacc gcctgggata cctcttcccc
     5821 cgccttcctc aaggaagtac acggaataaa gacgctgtac atccccaccg cgttcttctc
     5881 ctacaaaggc gaggcgctgg acaagaaggt tcccctgctc cgatccaatc aggcgatcga
     5941 gaagcaggcc ctgcgggtcc tgagggcgct gggaaacacc acggcgaaac ggatcaccgt
     6001 caatatcggc cccgagcagg agtatttcct agtcgacagg aaatactacg agcagcggac
     6061 cgaccttcgc ctcgccgggc ggaccgtcat cggatcgctg ggcgccaaaa gtcaggaaca
     6121 gaatgaccac tactacggta cgatcggcga ccgcgttgcg gtcttcatga gcgaactgaa
     6181 ctacgagctg tggaaactcg gcatcagcag caagacccag cacaaggaat cagcgcccaa
     6241 ccagttcgag atcgcggtgg tctacgatgc ggcgaacctt tccaccgacc acaaccagct
     6301 gttgatggat gtcctgcaga tggtcgccct tcgccacggg atggtcgccc tccttcatga
     6361 aaaacccttc gccggagtaa acggatcggg caaacacgac aactggtcgc tcgccacgga
     6421 agacggcacg aatctttatg ctcccggaac caacgtggag gacaacgtcc agttcctgat
     6481 cttcctcact tccttcatca aggcgatgga tgagtacgct ccccttatcc gcagcggcgc
     6541 cgccacggcg ggcaacgatc tgcgcctcgg ctccagcgaa gcgccgcctg cagtcatcag
     6601 catctatctc ggcgatcagc tcacgacaat tctggaaaca ctttcaaagg aaggcaactg
     6661 cgaaaaacgc gtcagggaag tggtgaagat gggaaccacg attcttcccg agctgccgaa
     6721 ggatctcacc gaccgcaatc gaaccagtcc gatcgccttt accggaaaca agttcgaata
     6781 ccggatggtc ggctcctccc agtcgatggc cggaccgaac atcttcatca acgccgcggt
     6841 cgcccaagtt ctcggcgaag ccgccgatcg cctggaagcg gcaacggaca aagaagcgga
     6901 atcgcacaag atcatcagcg atttctatca gaaccacaaa cgaatcatct tcaacggaaa
     6961 cggctactcg gaggagtggg cgaaagaagc gaaaaaacgg gggctccccc actacaggga
     7021 taccgtcagc gctcttcccc agat
//
LOCUS       DAOSLI010000072         6939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9902_length_6939_cov_18.994916, whole genome shotgun sequence.
ACCESSION   DAOSLI010000072 DAOSLI010000000
VERSION     DAOSLI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9902_length_6939_cov_18.994916"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..3362)
                     /locus_tag="PLF67_08280"
     CDS             complement(54..3362)
                     /locus_tag="PLF67_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE90044.1"
                     /translation="MITFKQFLERVSLYPDEDQMRAINADANCVVSAGAGSGKTMVLS
                     YRFVRLVLERKARFDQILTLTFTRKAAREMHERIHAHLSLCTDDEEIQAQLASFSSAP
                     ISTIDAFCAQVLKGSAHEHGLSPEFQVDDEANLNLAKRVAVELLERTAQDRGARMLSI
                     LYSPNTIEEVLLSLSAKYPMGVEFEAKSVADEIRSMIRKEYRRLLARFEELLNSYRDL
                     VQGGTGKTLEKFAEFTEVWLKILEEGEEEEIWKALAGNLGYFRGLGNVKDERLVSIKE
                     SGDEYHDIRRKLAVCAAFFLNEENLEPVVGFVARFLEGHRVQKRAASIVTFADISNLA
                     IKSLTDDKELRAFYKQAYRYIMIDEFQDNNEEQKRLLYLLAEKEGSEADGIPAVAGLS
                     RNKLFFVGDEKQSIYRFRGSDVSVFKALAKELRGIGGMSVKLPRNYRSEPGLITWYNE
                     IFPSIMKNEGEPFEADFEPLEFREPKEGIDPRITLAIKPYVENAEPAGEEMAPPPLAE
                     AYYLAKKIRHILNGDEYLIPTKNGSSRKPEPNEIAILLRSTSNQYHLERALRHLSIPY
                     TVQIARSLFLEAIINDFYAMLQLLIHPSDRLSYAVVLRSPFCFIDDNALADLLANGEL
                     FAVVDPLSELDKKKMVRMKRFFEDLKEAAAVQRLERLIFMLWHESGYAHHYIQNPDYH
                     SDIEHYELLYRLAQTFQEAGKTLSEFLDYLRNRLGQDERPGELDLIKETKEGVQIMTV
                     HKAKGLEFPIVIVANAGGTGLGSGNGPSSGHRYPLPNFFDQVILTGEKGKEERIGNID
                     ALFNDKTSGSQELAELKRLLYVALTRAETHLIISGFFHSKNRNLEKHLPSFLQHLCKA
                     LEIDPKNPSLEHGKVRLELIDDVPVREFRRGDPAPVPIEELKLWYESADAPYQAEALR
                     YAVTALAPEVERTFVKELPVIASDVILGEKECSALFGTFVHRLIEAHLKKEDLSDPLF
                     YMEDSFKELLTKRETEAILSDALLLSDNFIHSRLCADEVLGHPFKSEMSFFSRLQHEG
                     RTVVAEGAIDLLVDRGDEILVIDFKSDRFVDEEVHRFQIETYIDAARRLYKKKTGGLI
                     VYLRDITRSIRYEG"
     gene            complement(3359..5953)
                     /locus_tag="PLF67_08285"
     CDS             complement(3359..5953)
                     /locus_tag="PLF67_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOE90045.1"
                     /translation="MSILGDQLASNAVCVLPTEAARRSHLIEYALTSEDGVIAGASAL
                     SFDTFRASFLPTRADRIPANTLIRSIFAAEFIENGNPVASFYNPKFPESRHQAASVIA
                     SMLPSLREVLASEVTARIPGALFRQLQTVHAAYTEFLDRNNLFEPRYDSVSVPTDWNT
                     NQEVRILYSDLIPEAALLYEELGRPDWLKLVPTPKAKTPAIDVYANHLQEIRSTLRRI
                     RDLLDEGVATHEIMICPANSDELLATLEDEAFFYGIPLAIRQGRSPLEYPAGRFFTLL
                     NEVHGGHFTLTSLKNLLLEPAIPWKDRERIQTFIRLSLEDSILYGSAARPDYFETRLR
                     DRSLIRWYTTFRDSLSAIVQATSVAELIRSLSFFRDAFWEESEWHGTPQEEVFAHAMN
                     QIEQLDKALKACNRERLDGLYALFLSHLKRTQYVSRQQEEGIAVYRWPQGASIAGSHR
                     FFLALDQEGAEVLDDPYPFLPERIRHTLMKRKELGRQHLQAASLGDSILSCHRRSNSG
                     EKLIHFYFLEEGKVHKHGESLLLSKDPQSGELKRVLKKEGDQKPTSLQAQNFKSALLG
                     SLRRRKREHDHTRSPVEEHLRRRLYTEGRLPLSYEKIDAFAACPYTYLASSLYGIEPF
                     DYTEERINHLAIGVILHDAYERFFTSIGPFDSGKIESYAIDLQTAFDHALLKTYGTRG
                     PTASIRQWIIHTYRPLILKILEEEQTYFEHLRTSAIEKRLEWTDDRTSLRGRIDRIIE
                     MGRKQEDSWGVIDFKKGSVAAPVAGGDDMGVSSYQLLVYQALIERNDLGKVVLAAYYS
                     VAEGKYVFLYKQDEHEAQELCREALDRMLDTMLSAAEQGDFGVTQDTAVCDGCSFRAL
                     CRRRYSVP"
     gene            5985..6812
                     /locus_tag="PLF67_08290"
     CDS             5985..6812
                     /locus_tag="PLF67_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOE90046.1"
                     /translation="MEQKTRHLLFTLVTALLFFFGAGVYAASLLHPYLSAYLLANIPF
                     LALALGLVLSHSILLKEPLIDLVVETNAWSWRLALSSGLSYLAVLGLFSIIGAIRYPH
                     DYHVFHTPLWKRIGFIAMALVVTPIQTTGEEILFRIIPLKLMKKIQSTTIRALVPALI
                     FTLLHLANREIAQSQNRLAVLLYYALFGFIPSYIAVRSDRWEFPLAIHAANNLFVALF
                     CNYEHSSLPSLPLFISTRPIGTWMDTLQLAAALAISYSTIPQRGTKSNVEKTTILDA"
BASE COUNT         1422 a         2017 c         1959 g         1541 t
ORIGIN      
        1 ccgcatgctg ggtggtgtga gaggttgggg ccgtgaggcc cccgcctact cgattacccc
       61 tcgtagcgga tgctccgggt gatgtcccgg agatagacga tgaggccgcc ggtcttcttc
      121 ttgtagagcc gacgtgctgc gtcgatatag gtctcgatct ggaagcggtg aacctcttca
      181 tcgacgaagc gatcgctctt gaagtcgatg accagtattt catcaccccg gtcgaccaag
      241 aggtcgatcg ccccctccgc tacgacggtc cggccttcgt gctgcaggcg gctgaagaag
      301 ctcatctccg acttgaaggg atgaccgaga acctcatccg cacacaaccg gctgtggatg
      361 aagttgtcac tgaggagaag cgcatcggag agaatcgcct ccgtctctct tttggtcagc
      421 agctctttga aggaatcctc catatagaag agcggatcgg aaaggtcttc ctttttaaga
      481 tgggcttcga tcaggcggtg gacgaacgtt ccaaacaaag cggagcattc tttttcgcca
      541 aggatgacgt cgcttgcgat caccggcaac tccttgacga acgtacgttc aacctcggga
      601 gcaagagccg tcaccgcata ccggagcgcc tccgcctgat agggagcgtc ggcactctca
      661 taccagagct tcagctcctc aatcggcacg ggtgccggat caccacggcg gaactccctg
      721 acggggacat catcgatcag ctccaggcga accttcccgt gttccagact cggattcttc
      781 gggtcgatct caagggcttt gcacagatgc tggagaaacg atggcaaatg cttctcgagg
      841 ttccgatttt tggaatggaa gaatccgctg atgatcaggt gggtctccgc ccgggtaagg
      901 gcgacataga ggaggcgttt cagctcggcg agctcctgac tgccggacgt cttatcatta
      961 aagagtgcgt caatgttccc gattcgctcc tccttccctt tttcacccgt cagaatcact
     1021 tgatcgaaaa aattaggaag ggggtaccga tgaccagatg aggggccgtt cccgctgccg
     1081 agccccgtac ccccggcatt ggcgacgatg acgatcggga actcgaggcc tttggccttg
     1141 tgcaccgtca tgatctggac accctctttc gtttccttga tcagatccag ctcgccgggg
     1201 cgttcatcct gcccaagccg attccgaaga taatcgagga actcgctgag ggtcttaccc
     1261 gcttcctgaa acgtctgggc gagtcgatag agcagctcat agtgttcgat atccgagtgg
     1321 taatcggggt tctggatgta gtggtgggca tagccgcttt catgccacag catgaatatg
     1381 aggcgctcaa ggcgctggac ggcggcagcc tctttcagat cctcgaagaa gcgcttcatc
     1441 cgcaccattt tctttttgtc gagctcggaa agggggtcga cgacggcgaa gagttcccca
     1501 tttgcaagaa gatcggccaa ggcgttgtcg tcgatgaaac agaacggtga tcgaagcacc
     1561 accgcatagg aaagccggtc cgacgggtgg atcaggagct gcagcatcgc gtagaagtcg
     1621 ttgatgatcg cttccagaaa gagggaacgg gctatctgca ccgtataggg aatcgaaagg
     1681 tgccgcagag cccgctcgag gtgatactgg ttcgatgtgg agcgaagaag aatcgcgatc
     1741 tcgttcggct cgggctttct ggaagatcca ttctttgtgg gaatgaggta ctcgtcgccg
     1801 ttaaggatgt ggcgaatttt tttggccaga taatacgcct cggcaagggg tggtggtgcc
     1861 atctcctccc cggccggttc agcattctcc acatacggct tgatcgccaa ggtgatcctc
     1921 ggatcgattc cttcctttgg ctcccggaac tccaaaggct cgaagtccgc ctcgaagggt
     1981 tcgccctcgt tcttcatgat cgacggaaag atttcattgt accaggtgat caggccgggt
     2041 tcgctccggt agttcctggg cagcttgacg ctcatccccc cgatcccccg aagctccttc
     2101 gccagagctt tgaacacgga gacatcgctg ccccggaagc ggtagattga ctgcttctcg
     2161 tctccgacga agaagagctt gttccgcgaa agaccggcga cggcagggat gccatccgct
     2221 tcggaaccct ctttttccgc aagaaggtag agtagccgct tctgctcctc gttgttgtcc
     2281 tggaactcgt cgatcatgat gtagcgatat gcctgcttgt agaaggcgcg gagctccttg
     2341 tcgtcggtga gcgatttgat cgcaaggttg gagatgtccg caaaagttac gattgaggcg
     2401 gcccgcttct gaacccggtg tccttcaagg aaccgggcaa cgaacccgac gacaggctca
     2461 aggttttcct cattgaggaa aaaggcggca cagacggcaa gcttcctgcg aatatcatgg
     2521 tactcgtccc cggattcctt gatcgatacg agacgctcgt cctttacatt tccgagccct
     2581 ctgaaataac ccagattccc ggcaagcgcc ttccagatct cttcctcttc cccttcctca
     2641 aggatcttca gccacacttc ggtgaactcg gcgaattttt cgagggtttt tcccgttcct
     2701 ccctgcacaa gatcacgata ggagttcaag agctcttcga agcgggcgag caatcgccga
     2761 tattctttgc ggatcatcga tcggatctca tcggcgacgc tcttcgcctc aaactcgacg
     2821 cccatcggat acttggccga gagggagaga aggacttctt cgatggtatt cggcgagtac
     2881 aggatgctca gcatcctcgc cccccgatcc tgagcagtcc gttccagcag ctcgaccgcc
     2941 accctcttgg cgagattgag atttgcctcg tcatccacct ggaactcggg gctcagccca
     3001 tgctcgtggg cgctgccttt caggacttgg gcgcagaagg cgtcgatcgt gctgatcggg
     3061 gcgctggaaa aggaagcgag ttgggcttgg atctcctcgt cgtcggtgca gagggagagg
     3121 tgggcgtgga tccgttcgtg catctcccgg gcggctttgc gggtgaaggt caaggtgagg
     3181 atctgatcga agcgcgcttt gcgctccaaa accagccgca cgaagcgata gcttaagacc
     3241 atcgtcttcc cgcttcccgc tccggcgctc accacgcagt tcgcatcggc gttgatcgcc
     3301 ctcatctgat cctcatcggg atagagcgaa acacgttcaa gaaattgctt gaaggtgatc
     3361 atggtaccga atacctcctt cggcacaggg ccctgaagga acatccgtca cacaccgcag
     3421 tgtcctgagt aacgccaaaa tccccctgct cggcagcgct cagcatggta tccaacatcc
     3481 tgtccaacgc ttctctgcac aattcctgcg cttcatgctc atcctgtttg tacaggaaca
     3541 catacttgcc ctccgctacc gagtagtagg cggccaagac cacttttccc agatcgttcc
     3601 gctcgatgag ggcctgatag accaacagct gataggagga aacccccata tcatccccgc
     3661 ctgcgaccgg agcggccacg ctcccctttt tgaagtcgat gactccccac gaatcctcct
     3721 gcttccggcc catctcgatg atccggtcga tgcggccccg caaggatgtc cgatcatccg
     3781 tccattcgag cctcttttca atcgcggagg ttctgaggtg ttcaaaatag gtctgctcct
     3841 cctccaaaat cttaagaatc agggggcggt aggtgtggat gatccattgg cggatcgaag
     3901 cggtgggccc ccgggttccg tacgtcttca gcagcgcatg gtcgaaggcc gtttgaaggt
     3961 cgatcgcata ggattcgatc ttcccgctgt cgaaggggcc gatcgaggtg aaaaaccttt
     4021 cataggcatc gtgcaggatc actccgatcg ccaagtggtt gatccgttct tcggtataat
     4081 cgaacggctc gattccatac aggctgcttg ccagataggt atacgggcac gcggcgaacg
     4141 catcgatctt ctcgtaagaa agcggaagcc gtccctcggt atacagccgc ctcctaagat
     4201 gctcctcgac gggagaccgg gtatggtcgt gttcccgctt tcgccggcgc agcgagccga
     4261 gcagcgcgct cttgaagttt tgtgcctgca gcgaggtcgg tttctgatca ccttccttct
     4321 tcaggactcg tttcagctcg ccgctttgcg gatccttgct gagaagaagg ctttcgccgt
     4381 gcttgtgaac cttcccctct tcgaggaaat agaagtggat gagcttttct ccgctgttgg
     4441 agcggcggtg gcatgagagg atgctgtcgc ccagagaggc ggcctgaagg tgctgtcgcc
     4501 cgagctcctt ccgcttcatc agggtgtgcc ggatccgctc gggaaggaag ggatagggat
     4561 cgtcgagaac ttccgccccc tcctgatcga gggccagaaa gaagcgatgc gaaccggcga
     4621 tgctcgcccc ttgcggccag cgatagaccg cgatcccttc ctcctgctgc cgggaaacgt
     4681 actgcgtcct cttcaggtgg gagagaaaga gggcgtacag cccgtcgagg cgttccctgt
     4741 tgcacgcctt gagggcttta tcaagctgct cgatctggtt catcgcatgg gcgaagactt
     4801 cttcctgagg ggttccatgc cattcgcttt cttcccagaa ggcatcgcgg aagaatgaaa
     4861 gcgagcggat caattcggca accgaggtcg cctgaacgat cgcggaaagc gaatcgcgga
     4921 acgtcgtgta ccatcgaatc agggagcgat cccgcagtcg cgtctcgaaa tagtcgggtc
     4981 gagccgccga tccgtagagg atcgaatcct ccaagctgag gcggatgaat gtttggatgc
     5041 gctccctatc cttccagggg atcgccggtt ccaagaggag gttcttcagg ctcgtcaggg
     5101 tgaaatgacc gccgtgcact tcgttcagca gggtaaagaa gcgaccggcg ggatattcca
     5161 gcggcgaccg gccctgacgg atcgcaagcg gaatgccgta gaagaacgcc tcatcctcca
     5221 aggttgcaag tagctcgtcg ctgttggccg ggcagatcat gatctcatgc gtcgccaccc
     5281 cctcatcgag cagatcccgg atccggcgga gggtcgagcg gatctcctga aggtggttcg
     5341 catagacatc aatcgccggg gtctttgcct tcggcgtcgg aaccagcttc aaccagtccg
     5401 gcctgcccag ctcctcatac aggagggccg cttccggaat aagatcgctg tagaggatcc
     5461 gcacctcttg attcgtgttc caatcggtcg gaacggatac cgaatcgtat cggggttcaa
     5521 acaggttgtt tcgatcaagg aactcggtgt atgcggcgtg caccgtctgc agttgtcgga
     5581 acagagcacc gggtatgcgg gccgtgacct ccgaagcgag cacctcccgg agggagggta
     5641 gcatcgacgc gatgacactc gcggcctgat gccttgattc ggggaacttc gggttgtaga
     5701 aggaggcgac cggattcccg ttttcgatga actccgcagc gaagatggag cggatcaggg
     5761 tgtttgccgg gatccgatcg gcacgggtcg gcaggaagga agcgcgaaag gtgtcgaagc
     5821 tcagggcgga agcgccggcg atcaccccgt cttcactcgt taaagcatac tcaatgaggt
     5881 ggcttcgcct tgccgcctca gtaggaagga cgcacactgc gtttgatgcg agctgatcac
     5941 caagaataga catcccttgc agtataccgt acatttacaa aacaatggaa caaaaaaccc
     6001 gccatcttct gttcacgctc gttaccgccc tgctgttctt cttcggcgcg ggagtctatg
     6061 cggcctccct gctgcatcca tatctctcgg cgtacctttt agcaaacatc cccttcctcg
     6121 cccttgccct cggtctggtt ctttcccact cgattctctt gaaagaaccg ctcatcgacc
     6181 tcgttgtcga aacgaacgcg tggtcctggc gccttgccct ctcatccggg ctctcctatc
     6241 tggccgtgct ggggctcttt tcgataatcg gcgcgatccg ctatccccat gactaccatg
     6301 tgtttcatac gccgttgtgg aagcgaatcg gcttcatcgc catggcgctc gtggtcactc
     6361 cgatccagac caccggcgag gaaatcctct ttcggatcat tccgctgaag ctgatgaaga
     6421 agatccaaag cacgacgatt agggctctgg ttcccgccct catcttcacg cttctccatc
     6481 tggccaatcg tgaaattgcg caaagccaaa accggcttgc agttctcctc tactacgccc
     6541 tcttcggctt cattccttcc tatatagcgg tacgaagcga ccgctgggag ttcccgctgg
     6601 cgatccacgc cgccaacaat ctcttcgtcg ccctgttctg caactacgag cactcatccc
     6661 ttccatccct gccactcttc atcagcaccc ggccgatcgg aacctggatg gataccctcc
     6721 agctggcggc ggctctggcg atcagctaca gcacgatccc ccaaagggga acgaaaagca
     6781 acgtcgaaaa gactacgatc ttggatgcgt aggcggtttc gcttccatac tttgatgcga
     6841 ggatggcggt cgtcgatcag ccggcacacg agaaacgggg caagcgggat gaccagaagc
     6901 ttcaacacgg tgacatggat cagcggacga tcaacatgg
//
LOCUS       DAOSLI010000073         6935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_9909_length_6935_cov_23.514244, whole genome shotgun sequence.
ACCESSION   DAOSLI010000073 DAOSLI010000000
VERSION     DAOSLI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9909_length_6935_cov_23.514244"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1046
                     /locus_tag="PLF67_08295"
     CDS             42..1046
                     /locus_tag="PLF67_08295"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOE90047.1"
                     /translation="MKSPYLVVPFGDAAGIGVEIILKALGRVKEPIVVVGDRKLIAKT
                     AKDVGLPFPFTNIVTSTNQLRTAQIEGHTPILYHLDLIDLSTFAYGEIQAQCGRGAFE
                     SVRTAVDLIEEGLVLAVVTPPLHKEALKAAGIEEIGYTEILASLSDSPKPTTMFDTHK
                     LKIFFHSRHLSLIDACKAVTKERLLQTIVTSEEITGKYPAFFDTALPLAVAGLNPHNG
                     DGGLFGKEELLEVIPAIQAAQKQGINVVGPIGADSVFHQAKIGYYRAVISLYHDQGHI
                     AAKTYDFDNTISITWGLPFFRSSVDHGTAFDIAGKGIANEQGMVRALEAASFFVGGTT
                     "
     gene            1043..2095
                     /locus_tag="PLF67_08300"
     CDS             1043..2095
                     /locus_tag="PLF67_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90048.1"
                     /translation="MNRLRISLIIGALSVLLLSSCVSGLSITRQVPAEANLHGIQMLR
                     VEPVKEFFFLWRPVPAWVEGSSGQMELTLPSGYYSTLSEDVSYAATRTLERAIESTDH
                     LSVTSSRAAEAVIKSEITFMDLSERIIERSESEGSEEKSYFLVQSAAVALSIKVMEVS
                     TGSILYTSNYADKVTKEYLVGTRMFDPTSNTYKIIRRFTFTRSPSFLPLFEEILRSFE
                     RTIARQLAPSTVTEWVNLAPNRPKDAMAKEIYHLVDRGEYRLAYHRFLGLYEDTGDWR
                     WGYNAALLLQGIGELENAVVLMDEVYSGYPSNLVFQALSRMRDALRDQQKAEMQICGE
                     CLEEFTLVTQMYTEGR"
     gene            2099..2623
                     /locus_tag="PLF67_08305"
     CDS             2099..2623
                     /locus_tag="PLF67_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOE90049.1"
                     /translation="MVLIYTGNGKGKTSAAVGQAVRALGHDGKVALAQFIKQDPDLLD
                     SGEARILKELGVTWGSFGAGFTWIEGNEKENRILASKGWKTVKRWALSGVYDLIVLDE
                     FTYPLTLGYVDVEEIVSFITEHKDDEKFPHLVITGRGAPETLVAIADMVSEVGEVKHH
                     LANSGLEARPMIEF"
     gene            2705..3337
                     /locus_tag="PLF67_08310"
     CDS             2705..3337
                     /locus_tag="PLF67_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein CapC"
                     /protein_id="HOE90050.1"
                     /translation="MGLWDVHSHLLPEIDDGYLDRAGCIRMLEIYRECGIDHIVATPH
                     IYNPFVRTAVERIRSTFEWAKNEAEALGISLHLGSELYIADQGVLRAIPILDRFVLVE
                     FSLALPPPNLKEQLRKLIDDGFVPIIAHVERYVWLDPKHALVHKLKEMGCLLQGNVEA
                     VESGAALPYLEAELLDILATDNHGNERLPLSLVQTMEKWPFVKKRMEDIW"
     gene            3371..4618
                     /locus_tag="PLF67_08315"
     CDS             3371..4618
                     /locus_tag="PLF67_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90051.1"
                     /translation="MLLDMTIANFKSVRDEQTISFEAVRDNRFSDSKVVSVTEKLKLI
                     KSAAIIGPNGAGKSSFIRALEALKAIVTATEETNNPLAILSGTSFAYSEEKNQPATII
                     IRCILGSDEESGEPIIGVYTLVADRTKIYEESLHHVIGRSKRRMFERILKEDELALND
                     EELTYTYRWGKHYRGDKKRLVGKVDEKHTFIQASAEKGSETSNLFYSWIVDRLHLLPM
                     GVSNLSEKYLAQMIEEHPGWVSQLINFLWSVDITDIRDIRVKDDKVIFVHTNVTQHYA
                     SYFSLESLSLRRLCLIGVAFFESFTKGKTLIIDDFGMLLHPNVLTHIVEIFEANNDEN
                     GSQMLVVDCNPSLLKPGLLRRDGVYFAQKDSESATVYTSLANFRYSKANDRTQIQYMN
                     GAFGALPILSEFSYDRESAQDKE"
     gene            4621..5259
                     /locus_tag="PLF67_08320"
     CDS             4621..5259
                     /locus_tag="PLF67_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HOE90052.1"
                     /translation="MARKRLSQPPRKTILLVTASEIESLYFSQMRKDCRYSNMTVEHQ
                     PVFKDLHHLINLTARKRSAGGFTTVWLVFGLADLGLSAHDVRAELEHAKKKRVHLAWN
                     NPGLPLWFYLHFQEPKAFVRDMGAINGALEKAIDGYSYTSEYFLNDGDNFHLRLYSAK
                     SKAAVNGAMYNDRTRMEAGLEPTRMIALLNEITDICGLADLSRNQKQLGLKK"
     gene            5264..6157
                     /locus_tag="PLF67_08325"
     CDS             5264..6157
                     /locus_tag="PLF67_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOE90053.1"
                     /translation="MSGFLLLIYMLAFALGSMTLALAIVYRMAHKERWASFFIVCHAS
                     LLGAMMLLALQTFTKLFITGFGGQVFSLILRIVVLADAAFLIVFLPFFTSWVIAHPWR
                     QPYAALFPFLAAVYLGLGIVNQIRPLLFFEQAQFVLFVFVIGFCLVVLVRNLGSIRNK
                     IARTSALTIIIVSLSMVPAIMLALFFPGFKPFLYAVYFLALSITIMVFLFMEFVRLGR
                     EEKQHTRQLTVDDLAPYNITEREFEIITLISQGLTNKEIASELDISANTVTNHVANIF
                     SKTQVRSRIDLLNVVKQSLYQ"
     gene            6154..>6935
                     /locus_tag="PLF67_08330"
     CDS             6154..>6935
                     /locus_tag="PLF67_08330"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE90054.1"
                     /translation="MNLDYTFPPWTKGCYQDKAPVRTCAKPFTLTRPKAKNAALLIHG
                     YAGYPGELIRVGRELFEEGLDVYVPRLPGNGTSGGDFQKSRAKDWIGTLRNGMDDLTK
                     KYDDVLIAGHSMGGALAVTLAKEYSVSNVILIAPGLLIPFIPVFKLRLIAPFIKKIAV
                     PWQPDPEYTFHYESEEDDDQYLGTEYWSWAFPKTLLELEKVRKEAVNNLSNLKSNTLL
                     ITGGKDPIIPYDVVALVKEKIQGPFTHLHLEEAGHYIPYDKN"
BASE COUNT         1618 a         1852 c         1873 g         1592 t
ORIGIN      
        1 ggactctgga cagctctgaa gcgccttgtt atcatcgggc tatgaagagt ccctacctcg
       61 tcgttccctt tggggacgct gccggcatcg gtgttgagat cattcttaaa gcgcttgggc
      121 gcgtgaagga accgatcgtg gtggtcggtg accggaagct gatcgccaaa acggcaaagg
      181 atgttggtct tccttttccc tttaccaata tcgtaacctc aaccaatcaa ttgcgtaccg
      241 cccaaataga gggtcatact ccgatactct accacctcga tctcatcgat ttatccacgt
      301 tcgcctatgg cgagatccag gcgcagtgcg ggaggggagc gttcgaaagc gttcggacgg
      361 cggtcgacct gatcgaagaa ggattggtct tggcggtggt gactccgccc cttcacaagg
      421 aagccctcaa agccgccgga atcgaggaga tcggatacac ggagattctg gcttcgctga
      481 gcgacagtcc caagccgacg acgatgttcg atacccacaa gctgaagatc ttcttccaca
      541 gccgccacct ttccctgatc gatgcgtgca aggcggtgac gaaagagcgg ctgcttcaga
      601 cgatcgtcac gagcgaggag atcaccggta aataccccgc tttctttgat accgcgcttc
      661 ctttggcggt ggcgggactc aatccccaca atggggatgg aggactcttt ggtaaagaag
      721 agctgcttga agtgattccg gcgatccagg ccgcccaaaa gcagggaatc aacgtagtcg
      781 gtcccatcgg ggcggactcc gtattccacc aagctaaaat cggttactac cgggcggtga
      841 tcagcctcta ccacgaccaa gggcatatcg ctgcgaaaac ctatgatttt gacaacacca
      901 tctcgatcac ttggggcctt ccgttcttca gatccagcgt ggatcacggc accgcgttcg
      961 atatcgccgg gaagggaatc gccaatgagc aggggatggt cagagccctt gaggccgctt
     1021 cattctttgt tggaggtacc acatgaacag attgcgcata tccctcatca tcggagccct
     1081 ctcggttctc ctgttaagct cctgtgtgtc cggtctttca atcacccgcc aggttcccgc
     1141 cgaagccaat cttcatggaa tccagatgct tcgagtcgaa ccggtaaaag aatttttctt
     1201 tttatggcgt ccggttccgg cgtgggtgga gggaagtagt ggtcaaatgg agctgacgct
     1261 cccaagcgga tactattcca ccttgagcga ggacgtttca tatgccgcaa cccgaacgtt
     1321 ggagcgggcg attgaatcaa cggatcatct ctccgtcacc tccagtcggg ccgccgaagc
     1381 ggtgataaag agcgagatca ccttcatgga tctctcagag cggatcatag aacggagtga
     1441 aagtgaagga agcgaagaaa aatcctactt ccttgtgcag agtgcggccg tcgcgctctc
     1501 gatcaaggtt atggaagtat cgaccggatc gatcctgtac acctccaatt atgcagataa
     1561 ggtcaccaaa gaatatctgg tcggaacccg gatgtttgat ccgaccagca atacctacaa
     1621 gatcatccgc cggttcacct tcacccgaag cccctccttc ctgccgctct ttgaggagat
     1681 cctccgctca tttgaaagaa cgatcgcccg acagctcgcc ccctcgacgg ttaccgaatg
     1741 ggtgaatctg gctcccaaca ggccgaagga cgcgatggcc aaggagattt accatctggt
     1801 cgacaggggt gaatatcggc tggcgtatca tcggttcctc ggtctctatg aagataccgg
     1861 cgactggagg tggggctaca atgcggcgtt gctgctccag gggatcggcg aactggaaaa
     1921 tgcggtcgtg ttgatggatg aagtctattc aggctacccg tccaatctgg ttttccaagc
     1981 cctttcgcga atgagggatg ccctccgcga tcagcagaaa gcggagatgc agatctgtgg
     2041 ggagtgtctt gaggagttta cacttgtcac gcagatgtac acggaaggac gttgaccgat
     2101 ggtgctgatc tataccggca atggcaaagg aaaaacctct gccgcggtgg gacaggccgt
     2161 tcgggctctc ggacacgatg ggaaggttgc gttggcccag ttcatcaagc aggatcccga
     2221 tctgctggac agtggagagg cacggatact caaagagctt ggagtaacgt ggggatcgtt
     2281 cggtgccggc tttacctgga ttgaaggcaa tgagaaggaa aatcgaatcc tcgcctccaa
     2341 aggatggaaa acggtaaaac gatgggcgtt gagcggggtg tacgatctga tcgttttgga
     2401 tgaattcacc tatccgttga cccttgggta tgtcgatgtg gaagagattg tttcctttat
     2461 caccgagcat aaagatgatg agaagtttcc ccacctcgtg atcaccggac ggggtgcgcc
     2521 cgagacactg gtcgcgatcg ccgatatggt cagcgaggtc ggtgaagtga agcatcatct
     2581 ggcaaattcc gggctcgaag cccggccaat gattgaattt tgatggcctt gtaaggtaat
     2641 gagccctctt tttccttccc cttgtgagta attaagggta taataggcgc aaagggaggg
     2701 ctgtatggga ttatgggatg ttcacagcca tttgttgccg gaaatcgatg acggatactt
     2761 ggaccgggcg ggatgtatcc gaatgcttga gatttatcgc gagtgtggta tcgaccatat
     2821 cgttgcaaca ccccatatct acaacccctt cgtcagaaca gccgtcgagc ggatccgctc
     2881 tacattcgag tgggcgaaaa atgaagccga ggcgcttgga atatcactcc atctcggcag
     2941 cgagctgtac attgccgatc agggtgtctt gcgggcgatt ccgattcttg accgattcgt
     3001 ccttgtcgaa ttttcgctcg ctcttcctcc tcccaacttg aaggagcagc ttaggaagtt
     3061 gattgacgac ggctttgttc cgatcatcgc ccatgttgaa cgatacgtgt ggttggatcc
     3121 caaacatgcg ctggttcata agttgaagga aatgggatgt ctgttgcaag gcaatgtcga
     3181 ggcggttgaa agtggcgccg ctttgccgta tcttgaagcg gaacttcttg atatactggc
     3241 aactgataac catggtaatg agcgattgcc gttaagcttg gtccaaacga tggaaaagtg
     3301 gccattcgta aaaaaacgga tggaagatat ttggtaatac caccgaaggg tgagattgag
     3361 gaggtagcgc atgctgcttg atatgactat tgcgaacttc aaatccgtga gggacgaaca
     3421 gaccatttcg tttgaagccg tcagggataa ccggttcagc gattccaagg tggtctcggt
     3481 gactgagaaa ctcaagctca tcaagagtgc cgcaatcata ggtccgaacg gagccggcaa
     3541 gagctccttc attcgagccc tggaagcgct caaggcgatt gtaaccgcta cggaagaaac
     3601 gaataatccg ctagcgattc tcagcgggac ctcatttgca tactcggagg agaagaacca
     3661 acctgcaacg attatcatcc gatgcattct gggcagtgat gaggagagcg gggaaccgat
     3721 catcggtgtc tatacgctcg tggcggatcg gacaaagatt tatgaagagt ctctccatca
     3781 cgtaatcggc cgttcgaaac gacgaatgtt tgagcggatt ctcaaagaag atgagcttgc
     3841 cctcaatgat gaagaactga cctacaccta ccgttggggc aaacactacc ggggtgacaa
     3901 gaagcgcctg gtcggcaaag tcgatgagaa gcacacgttc attcaagctt ccgcggaaaa
     3961 gggcagtgaa acaagcaacc tcttctactc gtggatcgtt gacagactcc accttcttcc
     4021 gatgggagtt tcaaacctca gtgaaaagta tttggcccaa atgatcgaag agcatcccgg
     4081 ttgggtctcc cagctgatca acttcctgtg gagcgtcgac atcaccgata tccgcgacat
     4141 ccgggtcaag gatgacaagg tgatcttcgt ccataccaac gtaacacaac actacgcttc
     4201 atacttcagc ttggagagct tgagtctcag aaggctctgc ttgatcgggg tggcgttctt
     4261 cgagtctttc accaagggca aaactctcat catcgatgac ttcggcatgt tgctccatcc
     4321 gaacgtactg acgcacatcg tcgagatttt tgaggcgaac aacgatgaga acggttcgca
     4381 gatgctggtc gttgactgta acccgtccct gctcaagccg ggcttgctcc gccgcgacgg
     4441 tgtttacttc gcccagaagg acagcgagag cgctacagtg tacaccagtt tggcgaactt
     4501 ccgatattca aaagctaacg accgaaccca gattcagtat atgaacggag cattcggagc
     4561 tctcccgatc ctctcggaat tttcctatga ccgagagagt gcacaagata aggagtagtt
     4621 atggcacgca aacgtctttc ccaaccccct cgcaaaacga tcttattggt aaccgccagt
     4681 gagattgaga gtctgtattt ctcacaaatg cgtaaggatt gccgctattc caacatgact
     4741 gtcgaacatc agccggtgtt taaggatttg caccatctga tcaacctcac cgcccgcaag
     4801 cgaagtgccg gaggtttcac caccgtctgg ctggtcttcg gactcgccga tttgggtctc
     4861 agcgcccacg acgtccgcgc tgaacttgaa catgcgaaga agaagcgggt tcaccttgcg
     4921 tggaacaacc ccggtcttcc cctgtggttc tatctccact tccaggagcc caaggcgttc
     4981 gtccgggaca tgggggcgat caacggagcg ctggagaagg cgatcgacgg atactcctat
     5041 accagcgagt acttcctcaa cgatggtgat aatttccacc ttcgattgta ctcggcaaag
     5101 agcaaagcgg cggtcaacgg ggcgatgtac aatgaccgga cccgaatgga agccggtttg
     5161 gagccgacga ggatgatcgc tctgcttaat gagatcaccg atatctgcgg ccttgccgat
     5221 ctgtcccgaa accagaaaca gttgggattg aagaagtgac gccgtgagtg gtttcctcct
     5281 gctgatctac atgctggcgt tcgccctcgg gtccatgacg ctggcgctgg cgatcgtgta
     5341 tcgcatggcc cacaaggagc ggtgggccag ctttttcatc gtctgccatg cctcgttgct
     5401 tggagcgatg atgcttttgg cgctccaaac cttcaccaaa ctcttcatca ccggcttcgg
     5461 aggacaagtc ttttccttga ttctcaggat cgttgttctt gccgacgcgg cgttcctgat
     5521 cgtgtttctt ccctttttca cctcgtgggt gatcgcccat ccgtggcgcc agccctatgc
     5581 cgcgctcttt ccatttctgg cggctgtcta ccttgggttg gggatcgtca atcagattcg
     5641 tccgctgttg ttctttgaac aagcgcaatt cgttctcttc gtgttcgtga tcggcttctg
     5701 tctggtggtc cttgtccgga atctcggttc gatccggaac aagatagctc ggacaagcgc
     5761 gctgacgatc atcattgtaa gcctttcaat ggttccggcg atcatgctcg ccctgttctt
     5821 ccccgggttc aaaccgtttc tctacgcggt ctacttcctt gcgctttcga tcacgatcat
     5881 ggtctttctg ttcatggagt tcgtccgcct cggaagggag gagaagcagc atacccggca
     5941 gctgacggtc gacgatctcg ccccctataa catcacggaa cgggagttcg agatcatcac
     6001 cctgatcagc caaggcctca ccaacaagga gatcgcaagt gaattggata tcagcgccaa
     6061 cacggttacc aaccatgtgg ccaatatctt ctccaagacg caggtccgat cccggatcga
     6121 cctgctcaac gtcgtaaagc agagcttgta ccaatgaacc tcgactacac gtttcccccc
     6181 tggaccaaag gatgctatca ggacaaggcg ccggttcgca cctgtgccaa gccctttaca
     6241 ttgacccgac ccaaagcaaa gaacgcggcg ctgctcatcc atggatatgc cggctatccc
     6301 ggtgaattga tcagggtggg gcgggaactc ttcgaagagg ggctcgatgt ctatgttccg
     6361 cggcttcccg gcaacggaac cagcggcggg gatttccaaa agagcagggc gaaggactgg
     6421 atcggaaccc tgagaaacgg gatggatgat ctgacgaaaa agtacgatga tgtcctgatc
     6481 gccggccact cgatgggcgg cgccttggcg gtcacccttg ccaaggagta ttccgtttcc
     6541 aatgtgattc tcatcgcccc cgggcttctg attcctttca ttcccgtctt caagcttcgc
     6601 ctgatcgcac cgtttatcaa aaagatcgca gtcccttggc agcccgatcc cgagtacacc
     6661 ttccactacg aaagcgaaga ggatgacgac caataccttg gaaccgagta ctggtcctgg
     6721 gcctttccca agaccctcct ggagcttgag aaggtccgca aggaagcggt caacaacctt
     6781 tccaacctga aatcgaacac cctcctcatc accggaggca aggatccgat catcccctac
     6841 gacgtggtcg ctctggtcaa ggagaagatc caaggcccct tcacccatct ccatctcgaa
     6901 gaagccggac actacatccc ctacgacaag aacaa
//
LOCUS       DAOSLI010000074         6693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_10377_length_6693_cov_18.349503, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000074 DAOSLI010000000
VERSION     DAOSLI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6693
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10377_length_6693_cov_18.349503"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1527)
                     /locus_tag="PLF67_08335"
     CDS             complement(121..1527)
                     /locus_tag="PLF67_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90055.1"
                     /translation="MKITTRTIDTMEFQQLNDPITTVFPGKTYESRISRAIAKGKQEG
                     YSHLVIYGDREHFANIHYFTGFDPRFEEALLILSEGKKPLLLVGNEGLWYSKIIPFEL
                     DVMLFQSFSLVGQPRSKDTSNVLKDAYERVGLCKTSKVGVIGWKYFTDTEAVDPKYMF
                     DVPHFIVESLKAETGAHNLENAAKLLIGAEGGMRTNLDVDELAVLELAGTKTSRSVLN
                     MLMHLRPGISEIEASTYLKIDGNPLVAHPNVNFTTEGILQGLASPGTHTLEYGSVCNV
                     GFGYRSSMVARTGLYTSRVEDIKKKEFHDVWDKVYVPYFKILALWYGSVAIGVSGRHI
                     VEKIRKEVPEFDRLGIGLNPGHLIHNDEWTDSLFVHDEDIHLRSGMAIQCDIIANPEG
                     LPGVHVEDGLALADAKLRDAFKNKYPEAWKRIERRREVMTDVLGLELSDDILPFSDIQ
                     GMLFPWMADLKTVMAVEG"
     gene            complement(1544..2428)
                     /locus_tag="PLF67_08340"
     CDS             complement(1544..2428)
                     /locus_tag="PLF67_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HOE90056.1"
                     /translation="MFGLIVHGGCHDLEPTELDKVSANNGVKTYGEIGYKMLSEGCAA
                     IDVVEKVITMMEDDPIFDAGTGSFRNLNGVVEMDAMVMNSEENCGAVHCIQKVRNPIQ
                     VARMVMEKTPHSILTGSGAVQFARLHGFEEYEPALSDVKNPSEEIKGRERSLRDIQYY
                     VEEIRKEDHIFSTVGVVALDQAGRLVAGTSTGGIRMKMPGRVGDSAIPGAGTYCTREI
                     GLSATGEGEKILRMCLTYQSARDYLNTGKLTDSLRKNVKRASEIDCICGLIAFTRDGE
                     FSFAHNGAFMPVYYRTNK"
     gene            complement(2454..3194)
                     /locus_tag="PLF67_08345"
     CDS             complement(2454..3194)
                     /locus_tag="PLF67_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOE90057.1"
                     /translation="MTMAMIEVKNITKAFGELVVLKNFSVDFDQSGVTVILGPSGTGK
                     STLLRCINGLETIDSGDILVDGLSVREKKNLKDIRIKCGMVFQQTILFPHLDVLENLV
                     ISPIKILGRPRNEAIEQAKELLNKVGLMDKLHSKHNELSGGEQQRIAIARAMMMNPKA
                     LLLDEITSALDPEMSTEVLKIIEKLAKDGVVMLIVTHEIPFARRIANRVLFLEYGSVV
                     SDTSNEEFFTTVKNNNERIARFIQNIHH"
     gene            complement(3213..3875)
                     /locus_tag="PLF67_08350"
     CDS             complement(3213..3875)
                     /locus_tag="PLF67_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOE90058.1"
                     /translation="MFEQYLSLTHYDIYFFLKAIGMTLWITLVSITIGTLIGIVMGLI
                     RCSKNKIVSAIPLIYIEPLRNSPLVTQLFLTYYGLPVVANIVLDAQAAAIITLSLNTG
                     AFFSVLVHSAVKAIPRLQWEAGFALGHNRYSNFKNIIAPQVLRLLIPSAITLYINQLQ
                     VSSFVSLISLIDLTKAGHILTLRTMRPFLIYGIVFVLYFAISYPLSKLAQRLEKKVAF
                     NF"
     gene            complement(3896..4552)
                     /locus_tag="PLF67_08355"
     CDS             complement(3896..4552)
                     /locus_tag="PLF67_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOE90059.1"
                     /translation="MYLNFSFLKTFYPMLLQGARLTLIISAGALVLGFILGIIVALMR
                     QSKSKVLRFIGSAWVEFLRNTPFLVQLFFLYFGLPELGIHTDPIVTSILALGINSSAP
                     NCEVIRSGLLAVKKGYYECSYALGYNSFQTFRYIVLPVSLRLAFKPLTSNFINLVLTS
                     SVVFSITVNDLMGSSKTVAARTARPFEVFIFIMLAYCVFTFVISFVAKAIDRRISITL
                     "
     gene            complement(4636..5340)
                     /locus_tag="PLF67_08360"
     CDS             complement(4636..5340)
                     /locus_tag="PLF67_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOE90060.1"
                     /translation="MDRGVLRAGIALNGPPIGGRDDKGQPYGYDVDFAQKFADTLGVK
                     LEITDVDGETRIPALTSGRVDIVFANMTGNLERAKSINFSMPYLRCGIKMMVRQGSPY
                     QTVEDLNKPGIKIAVARGTTGEDLALKYAPQAELVYVTNFTDQTLLMKQGKADATFED
                     STLIDFTAGQSKGELVALPKLYTSDPICIGLPKGDMEFVRYVDMFVSWMISSGFQRET
                     YMKWWGTEPTEYLAIW"
     gene            5645..6691
                     /locus_tag="PLF67_08365"
     CDS             5645..6691
                     /locus_tag="PLF67_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE90061.1"
                     /translation="MADRITQRNVADLANVSTGTVSRVINGHPLVSEEARAAVLRAID
                     QLGYIPNKAAQSLAQGRTRNILVAFLDKSPILPSTWQYELPILQAINDYLRLNDYSVQ
                     ITIHTAENNLSSILFKDVMYNKSIDGMIILTSLAVEDSFIRSLEESRIPAVFIGNGPF
                     WHDGNQVGTAILFDNFYIIQDAYNMLRSLGHERIGFITGSDEHIHSQIRLQAFTEIAA
                     KHMDSGTYEQLTYHGDYSINSGFEALYHFFKKSDPPTAIICANDLMAIGAMKAAKELH
                     MRIPQDLSIIGFDNIEVGSFYSPPLTSIKVPSYDLGRQGAELLLETIKDGKTKEPITL
                     PTELIIRKSISKMI"
BASE COUNT         1725 a         1724 c         1492 g         1752 t
ORIGIN      
        1 ggatcgtaaa agaacagaat cttcagggca ggcaagctct gcagattctg ttttttcatt
       61 gacgaaggcg tcagaatact tcttgttagt ggggatgctc ttgattgttc ccggtttctc
      121 ttatccttca accgccatga ctgtcttgag gtcggccatc catgggaaca gcatgccttg
      181 gatatccgaa aatggcagaa tatcgtcact gagctcaagt ccgagaacat ccgtcatcac
      241 ttctcttcgt ctttcgattc ttttccatgc ctccgggtac ttgttcttga aagcatccct
      301 cagctttgca tcggcaagag ccaatccatc ttcgacgtgc actccgggca acccttccgg
      361 attggcgatg atatcgcatt ggatcgccat gccgcttctc aaatgaatgt cttcatcgtg
      421 tacaaagagg ctatcggtcc attcatcatt atgaatgaga tgtcccggat tcaggccgat
      481 gcccagccgg tcgaactcgg gaacttcctt cctgattttt tccacgatgt gtctgccgga
      541 caccccgatg gctactgacc cataccaaag agcaagaatc ttgaaatagg gaacatatac
      601 tttgtcccac acatcatgga actctttttt cttgatatcc tcgacccggg aggtgtaaag
      661 tccggttctc gcaaccatcg aactgcgata cccgaacccc acgttgcaga ccgaaccgta
      721 ttccaatgta tgcgttcccg ggcttgccag tccctgaagg atgccctcgg tcgtaaagtt
      781 cacgttggga tgcgccacca agggattgcc gtcgatcttc agatacgttg aagcttcgat
      841 ctcactgatg cccggccgca aatgcatcag catgttcaac acgcttcgag acgtcttggt
      901 tcccgccagc tcaagtaccg ccaactcgtc cacatcgaga ttcgtgcgca ttccaccctc
      961 ggcaccgatg agcagtttcg ctgcattctc aaggttatgt gctccggttt cggccttcag
     1021 ggattcaacg atgaaatggg gaacatcgaa catgtatttc ggatcgacgg cttccgtatc
     1081 ggtaaaatat ttccatccga tcacaccgac tttgctggtt ttgcaaagac ccacccgttc
     1141 atatgcatcc ttcaacacgt ttgaagtatc cttggaccgt ggttgtccga ccaggctgaa
     1201 gctctggaaa agcatgacat ccaattcaaa gggaatgatt tttgaatacc acaggccttc
     1261 attacccacc aggagaagag gttttttacc ttcggacaga atgagaaggg cttcttcaaa
     1321 ccgcggatcg aaacccgtga aataatggat attcgcaaaa tgctcgcgat cgccatagat
     1381 cacgagatga gaatatcctt cttgctttcc tttggcgatg gctcgggaaa ttctcgattc
     1441 gtaggttttg ccgggaaaga cggtcgttat cggatcattc agctgctgaa actccatcgt
     1501 gtcaattgtt ctcgtcgtga tcttcatgca attaaccctc ttgttatttg ttggtgcgat
     1561 aataaacagg catgaaagcg ccattatgtg caaaggagaa ttcgccgtct cttgtaaaag
     1621 cgatcaagcc gcagatgcaa tcgatttccg aagctctttt cacatttttt cttagtgaat
     1681 cggtcaactt tcccgtattg agataatccc gggcggattg ataggtaaga cacattcgca
     1741 ggattttctc tccctcgccg gtcgccgaga ggccgatctc ccttgtacag tatgtccccg
     1801 ctcccggaat ggcggaatct cctacccttc ccggcatttt catccggatc cctcctgtgg
     1861 acgttcccgc gacaagtcta cctgcctggt caagcgcgac gactcccacc gtggaaaaga
     1921 tatgatcttc cttccggatc tcttcgacat agtattgaat atctcttaac gatcgttcac
     1981 gtcccttgat ctcttcagat ggatttttca cgtcactgag agccggttca tactcttcaa
     2041 agccgtgaag ccttgcaaac tggactgcac cgcttcccgt gagaatggaa tggggggtct
     2101 tctccatcac cattctggcg acttgaatgg gattcctgac tttctggatg caatgcacgg
     2161 ctccgcagtt ttcctcgctg ttcatgacca tcgcatccat ttccacaaca ccgttcagat
     2221 tgcgaaagga gccggtgccc gcatcgaaga tgggatcgtc ctccatcatc gtaatgactt
     2281 tttcaacgac gtcgatggcg gcgcatcctt cggagagcat cttatatcct atttcgccat
     2341 atgtttttac accattattt gcagaaactt tatcaagttc ggtaggttcc aaatcatgac
     2401 atcctccatg cacaatcaac ccaaacattt catctctccc gtcaatcaaa agatcagtgg
     2461 tgaatatttt gtataaatcg agctatccgc tcattgttgt tttttacggt agtgaaaaat
     2521 tcttcattgg aggtgtccga tacgaccgat ccgtattcaa ggaacagcac tctgtttgcg
     2581 atgcggcggg caaaaggaat ttcatgggtg acaatcagca tgactacgcc gtcttttgca
     2641 agtttttcga tgatcttcaa gacctcggta ctcatctcgg gatccaaggc tgaagtaatt
     2701 tcatcaagca acagagcctt tggattcatc atcatcgctc ttgcgatggc gattcgttgc
     2761 tgttctcctc cggacagctc attatgcttg gaatgcaatt tgtccatcaa tccgacttta
     2821 ttcagcaatt ccttcgcctg ctcgatcgcc tcattccgcg gtcgcccaag aatcttaatc
     2881 ggactgatga ctaaattctc caatacatca agatgaggga acagaatcgt ctgttgaaaa
     2941 accattccac atttgatccg aatgtccttt aaattctttt tttcccgaac cgacaaccca
     3001 tctacaagaa tgtcgccgct gtcgatggtt tccaaaccgt tgatacacct caaaagcgtg
     3061 ctctttccgg ttccgctcgg cccaaggatg accgtaactc cgctttgatc gaaatcgacg
     3121 ctgaaattct tgagaacgac taattctccg aatgctttcg ttatattttt tacttcgatc
     3181 atcgccatcg tcatgtacct cacatataaa agctaaaaat tgaatgccac ttttttctca
     3241 agccgctggg caagtttcga cagcggatag gatattgcaa aatataatac gaagactatt
     3301 ccgtagatca agaaaggtcg catcgttctc aatgtcagaa tatggccagc ttttgtaaga
     3361 tcgatcaaac tgatcaaaga aacaaacgaa gatacctgca gctggtttat gtacagcgtg
     3421 atcgccgaag gaatcaacag cctgaggacc tgcggcgcga tgatgttttt aaaattcgag
     3481 tacctgttgt ggcccaaggc gaatccggct tcccactgca gtctgggaat cgccttgacg
     3541 gcgctgtgaa caagcacgga gaagaacgct cccgtattca atgacaaggt gatgatcgca
     3601 gccgcctgtg catcgagaac aatgttcgca accaccggca atccgtaata cgtaaggaaa
     3661 agctgcgtga cgagcggaga gttccgcagt ggttcaatat agatcagagg aatggctgaa
     3721 acgattttat tctttgaaca acggatcaac cccatcacga ttccgatcag ggtgccgatg
     3781 gttatcgaga ccaacgtgat ccagagcgtc atgccgatcg ctttcaagaa aaaatagata
     3841 tcataatgtg tcaagctcag gtactgttca aacacaatag cttccttata tcaccttaga
     3901 gcgtgatgga aatccttcta tcgatcgcct tggccacgaa cgaaatgacg aacgtgaaaa
     3961 cacaatacgc caacatgatg aagatgaata cttcaaaagg tctggcggtc cttgccgcca
     4021 cggtcttcga acttcccatc aaatcgttga cggttatgga aaacacgacg gatgaggtaa
     4081 gcacgaggtt tatgaaattg cttgtcaacg gcttgaatgc cagacgcaag gataccggca
     4141 gtactatgta tctgaaggtc tgaaacgagt tgtaccccaa tgcgtagctg cattcataat
     4201 agcctttctt caccgccagc aatcctgatc ggatgacttc acagttcgga gcgcttgagt
     4261 tgattcccaa tgccagtatt gaagtgacga tcggatcggt atgtattccc aactcgggaa
     4321 gtccgaaata caaaaagaac aattggacga gaaacggcgt atttctcaga aattccaccc
     4381 aagccgaccc gatgaatcgg agcactttgc ttttggattg ccgcatcaat gcaacgatga
     4441 ttcccagtat gaatccaagg accaatgctc ctgcggagat gatcaacgtt aaacgagccc
     4501 cctgcagcag catcggatag aacgttttta gaaaactgaa attcaaatac atgagcacac
     4561 ccttccgaca gccgcagaag cagcccttgt gagaagcggc gttcccacaa gagctacaaa
     4621 atcaaacttc attatttacc agatggcgag atactccgtc ggttcggtgc cccaccattt
     4681 catgtacgtc tccctctgga aaccggaact gatcatccag gagacgaaca tgtcgacata
     4741 gcggacaaac tccatgtccc ccttgggaag tccgatgcag atcggatccg aggtatagag
     4801 tttaggaagg gcgaccaact cgcctttgct ctgacctgcg gtgaagtcga tcagcgtgct
     4861 gtcttcaaac gtcgcatccg ctttcccctg cttcatcaac aatgtctggt cggtaaagtt
     4921 cgtcacatat accagctcgg cttgaggagc atacttgaga gccagatctt cacccgtcgt
     4981 tcctcttgcg acggcaattt tgattccggg cttgttcaga tcctcgaccg tctggtatgg
     5041 actgccttgc ctgaccatca tcttgattcc gcagcgcaga taaggcatcg agaagttgat
     5101 tgacttggcc cgttcaagat tgcctgtcat gtttgcaaag acgatatcaa ctcggccgct
     5161 cgtcaaagcc gggattctcg tttctccatc gacatcggta atctcaagct tcaccccgag
     5221 tgtatctgcg aatttctggg caaaatcgac atcataccca taaggctgtc ccttatcgtc
     5281 ccgcccgccg atgggaggac cattaagagc tataccggca cgcaagacac ctctgtccat
     5341 gattgtttgc aacatgctga cactttttgc aggtgagctg tcttgaggct ggctcggagc
     5401 ttttccaccg gaacatgaca gcagaactaa cagaaaggcc accaataacc caacgaataa
     5461 tttccgtttc ataaaaacct cctcttggtt tcgcacacta ctgtatgcgc ttacattcta
     5521 aggagtgatt ccaagggttg tcaatgaaaa taatgttgtt ttttctttgc acaataataa
     5581 gtttatgata gtctttacta tgattaagcg tatgcaatca cgcttgattt gaaggagttc
     5641 atggttggct gatagaatca cacaacgcaa tgtggctgat ctggcgaacg tttccacggg
     5701 tacggtttcc cgggtgatca atgggcaccc gctggtcagt gaagaggcga gagcggcggt
     5761 tctccgtgcg atcgaccagc ttggatacat tccgaacaag gccgcccaat ccttggcgca
     5821 aggtcgtaca aggaacattc tggtagcctt tctggacaag agcccgatcc tcccgtcgac
     5881 atggcagtat gagctgccaa ttctccaagc cataaatgat tatttgcgat tgaatgatta
     5941 ctccgtccag ataacaatac atactgccga gaacaatctg agttccatcc tattcaagga
     6001 tgtcatgtac aacaaatcca tcgatggcat gatcatcctt acatccttgg ctgtcgagga
     6061 cagtttcatt cgatctttgg aagaaagtag aattccggca gtgttcatag ggaacggccc
     6121 gttttggcat gatggaaacc aggtcggtac agccatactg tttgacaatt tttacatcat
     6181 ccaggatgcg tacaacatgt tgcgttcatt aggccacgaa aggattggtt tcattacggg
     6241 ctctgacgaa catatccact cgcaaatccg attgcaagcg tttacagaga tcgctgcaaa
     6301 gcacatggat tccggaacat atgaacagtt gacgtatcat ggagactact ccatcaattc
     6361 gggctttgaa gcgctctacc atttttttaa aaagtccgat ccgccaaccg ccatcatatg
     6421 cgccaacgac ctcatggcga tcggggcgat gaaagccgca aaggaactcc atatgcggat
     6481 accccaagat ctctcgatca tcggatttga caatatcgag gtgggaagct tctattctcc
     6541 cccgctgaca tcaatcaaag tcccgtcgta tgatcttggc cgacaaggag cggaactctt
     6601 gctggaaacc atcaaggacg gcaagacgaa agaacctatt accctgccga ccgaactcat
     6661 cattcgaaaa tccatcagca agatgatcta gca
//
LOCUS       DAOSLI010000075         6604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_10563_length_6604_cov_30.585738, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000075 DAOSLI010000000
VERSION     DAOSLI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10563_length_6604_cov_30.585738"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..401
                     /locus_tag="PLF67_08370"
     CDS             15..401
                     /locus_tag="PLF67_08370"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOE90062.1"
                     /translation="MGEKKHLSAEFVPGKWEFTTDGGSTFQIGKTSTAYDYLMGALSG
                     CLFATFNDLAIKMKVEWEHVSFDVNGEKKATPPTTFEWVSIDVVAKGVADQARFTKAF
                     ETATRYCSIYTTISKVAEMKWNIEFA"
     gene            398..907
                     /locus_tag="PLF67_08375"
     CDS             398..907
                     /locus_tag="PLF67_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90063.1"
                     /translation="MSAKNRYSDIMLFDAVRKSLGSFLSSDEILLDWFKPKASVAHAL
                     ANHLYSSLGIEESDSLWVDVGVNGADIMVHDRSGKQILGIIFSFTYLSSSQQGQLLLL
                     EQGGCRMTLGLAFLPQKEYILTYRSKKGRLDYYHYVKPSGEMNKLKEKEVRTGIDTGQ
                     LTLGIKRGR"
     gene            complement(891..1619)
                     /locus_tag="PLF67_08380"
     CDS             complement(891..1619)
                     /locus_tag="PLF67_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOE90064.1"
                     /translation="MKIQLETIPVWDGLNSGSECYICDLMKEAEEDALTFYLGPSVMN
                     PETRVRVNEHGFCHAHWNALVARKKAQAVALMSDTYLKRTRELLEGDINNLRKAKTAN
                     QVKKAVKKFAESLNKREAGCLVCTSMENRLERYLYTTCYLWGDDPEFATALSKGKGFC
                     LHHAGLLLQTAPKALSSDKVAEFTRSLVETQLKNLDRIAEDIHWMTQKYKSENKDKPW
                     GTGEDAHKRGVEKMVGTVRVIDPV"
     gene            complement(1616..2281)
                     /locus_tag="PLF67_08385"
     CDS             complement(1616..2281)
                     /locus_tag="PLF67_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOE90065.1"
                     /translation="MDISHFGNERTSVAEFMRRLYDRQLTTASGGNISLRINEELFCI
                     TPSALDKGLLEKDQIAVVSLEGENLTPHLKLSIEADMHRLILRARPDVKAVFHAHPTY
                     ASAFTAISRDGKPVINTRLLAESYFILEDPVFVEYRLMGTDDLARLTAEKAKNHDVLL
                     LENHGVVCLGKSMLEGFDKIELLERAAQMTLATVQMREAGYTVSSLTEERMKQLMVMK
                     GKA"
     gene            2364..4136
                     /locus_tag="PLF67_08390"
     CDS             2364..4136
                     /locus_tag="PLF67_08390"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOE90066.1"
                     /translation="MRMSQLFSKTLREAPAGADSKGYEYLLRAGFIRQLGAGIFSLLP
                     LGYSSVKKIEQIIREEMDAIGGQEILMPVVNPADIWKETGRFYSIDREMSRFKDRVGR
                     DMVLAMTHEEVVTDIARFEIDSYKRMPRLVYQIQTKWRDDPRPRAGLIRVREFTMKDS
                     YSFDIDQAGLDKQYAAHYLAYFKIFGRTGLPTIVVGADSGMMGGKISHEYMYLSPIGE
                     DTIITCDSCSYTANRQVATFRKEHFPEAMKELEKVHTPECPTIEALAEYLKISTRQTA
                     KAVFMMGTFIDDTNGEEEQKLVVGVIRGDLEIEENKLQNAAKANALRPAHSEEILAAG
                     IVPGYGSVIGIRQDKILVIADDSVAKSNNLVAGANEEGYHYLNSNFTRDWNGTVADIA
                     SAQAGFICAECGGTLKSSKGVEVGNIFQLGTRYSESMDCYYQDENGIQKPVIMGSYGI
                     GVGRLLACLAEEYHDELGLNLPVTVAPYQVHLVSLVKDAEIAEELYRRLTDAGITVLY
                     DDRKESAGVKFADADLIGLPIRITLGNRSLKEGKVEVKVRSNPDEQSSFDLATLVDEI
                     KALIKRLENQITDSMYLEEWQPEK"
     gene            complement(4175..4687)
                     /locus_tag="PLF67_08395"
     CDS             complement(4175..4687)
                     /locus_tag="PLF67_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE90067.1"
                     /translation="MSKRIDALNRKLLAAATSADKAKEIRDLLKEGADIHVQNDWGLT
                     PVMLAAQYNPSVVVLKALLEAGADIHESEPKYRSNALHLAANRSTNVKVIEALLEAGA
                     DLNARNYLGETALIIAVNTNPETRIVSALLKAGADFNARDYQGHSVLEYAREAKRTYI
                     VTQLRKLGAQ"
     gene            4808..5893
                     /locus_tag="PLF67_08400"
     CDS             4808..5893
                     /locus_tag="PLF67_08400"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOE90068.1"
                     /translation="MLTSAIIKQLPKVELHDHLDGGVRVETIIELASEYGIALPSTDP
                     KSLHEWFARGGKQKSLTLYLRAFGVTTSVMQTKEALKRIAFEAVEDLAEDRVCYAEIR
                     FAPNLHTAKGLTMEESVQAVLDGLDQGTRKTGVPIGLILCAMRNQSPAVSLEVAQLAV
                     AFADRGVVGFDLAGDEAGNPPKKHLDAFQFIRSKNFNITLHAGEAFGVESIWQAVQIC
                     GSHRIGHGVRLVEDMTIEGSKILEMGSLANYILDRRIPMEMCLSSNVGTGAVASLSKH
                     PFPILFRNRFRVFLCVDNRLMSDTTLSKEMELAVEHYNLTIRDLEKITINAMKSAFIH
                     HEERLKIIYDVIKQGYAQVRKQYEILD"
     gene            complement(5890..6528)
                     /locus_tag="PLF67_08405"
     CDS             complement(5890..6528)
                     /locus_tag="PLF67_08405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOE90069.1"
                     /translation="MKKPTVLNRRFKETIYRRFAIKIIILNVFVFLVTDYLWPKGFYY
                     LAMIPSFVRHGYLYQLFTYMFVHGSFNHLIFNMLSLLIFGTMVEKRIGSKEFLLFYLL
                     TGLFSGIISFISYYLGGMNVVLVGASGAIYGVLLLFAVFFPYSVIFVFGIIPVRAPIL
                     IIIYALIELYSQVFGRGGNVAHLTHLSGLAFAYLYCRIRMRINPFEVFRRTL"
BASE COUNT         1535 a         1842 c         1819 g         1408 t
ORIGIN      
        1 tacaggagct atagatgggt gaaaagaaac atttaagtgc agaattcgtt cccggaaagt
       61 gggagttcac cactgatggg ggaagcacct ttcagatcgg aaagacctcc accgcctatg
      121 attatctcat gggtgcgctt tcggggtgtc tcttcgccac gttcaatgat ctggcgatca
      181 agatgaaagt cgaatgggaa catgtgagct tcgatgtgaa cggtgaaaag aaggcgacac
      241 cgccgacgac cttcgaatgg gtttcaatcg atgtcgtggc caaaggggtt gccgatcagg
      301 cgcgcttcac caaggcgttc gaaacggcca cgcgctactg ttcgatctac accaccatca
      361 gcaaagtcgc tgaaatgaag tggaacattg aatttgcatg agcgcgaaga atcgatactc
      421 cgatatcatg ctctttgatg ccgtcaggaa aagtctgggg tcttttcttt caagcgatga
      481 gatccttctt gactggttca aaccgaaagc cagcgtcgcc catgcgttgg ccaatcactt
      541 gtacagctcg ctcggcatcg aagagagcga ttcgctctgg gtggacgtgg gggtcaatgg
      601 cgccgacatc atggtgcatg accgatcggg gaagcagatt ctgggaatca tcttctcctt
      661 cacctacctc tcctccagcc agcaggggca gttgcttctt ctggaacagg gagggtgcag
      721 gatgaccctc ggcttggcgt ttctcccgca aaaggagtac atcctcacct atcggtcgaa
      781 gaaaggccga ttggactact accactatgt gaagccgagc ggggaaatga acaagctcaa
      841 ggaaaaggaa gtccgaaccg gcattgatac aggtcaattg acgctcggca tcaaacgggg
      901 tcgatgaccc gcaccgttcc caccatcttc tccacgcccc gtttatgggc atcctcgccg
      961 gtaccccaag gcttatcctt attttccgac ttgtacttct gcgtcatcca gtgaatatcc
     1021 tcggcgatcc gatcgaggtt cttcagctgt gtctcgacca gagagcgggt gaactcggcg
     1081 accttgtcgg acgaaagcgc tttgggcgcc gtctgaagca aaaggcccgc atgatgaaga
     1141 cagaaccctt tccccttcga caacgctgtc gcaaactccg gatcgtcccc ccacagataa
     1201 caggtcgtat agaggtagcg ctcaagccgg ttttccatcg acgtgcagac gaggcacccc
     1261 gcctctcgtt tgttgaggga ttcggcaaac ttcttcaccg cctttttgac ttgattggcc
     1321 gtctttgctt tcctgaggtt gttgatatcc ccttcaagga gctctctggt ccgtttgagg
     1381 taggtatcgc tcatcagggc gaccgcctga gctttcttcc tggcaacgag ggcgttccag
     1441 tgggcatgac agaatccatg ctcattgacc cggacccgag tctccggatt catcaccgaa
     1501 gggcccaagt agaacgtaag cgcatcttct tccgcttctt tcatgaggtc gcagatatag
     1561 cattcgcttc cactgttcag cccgtcccat accggaatcg tctcaagctg gatcttcatg
     1621 ccttgccctt catcaccatc aactgcttca ttcgttcctc ggtcaatgag gaaaccgtgt
     1681 agcccgcttc ccgcatctga accgtcgcga gggtcatctg ggcggcccgc tccagcagct
     1741 cgatcttgtc gaacccctca agcatgctct tgcccagaca caccaccccg tggttttcaa
     1801 gaagaagcac atcgtggttc ttcgccttct cggcggtcaa ccgggccaga tcatcggttc
     1861 ccatcaaccg gtactcgacg aacaccggat cttcgaggat gaagtacgat tcggcaagca
     1921 ggcgggtgtt gatcacgggc tttccatccc tgctgatggc ggtgaatgcc gaagcgtagg
     1981 tcggatgggc gtggaagacc gctttgacat cgggacgggc ccgaaggatc aggcggtgca
     2041 tgtccgcttc gatcgagagc ttcagatggg gcgtgaggtt ttccccctcc agggagacga
     2101 ccgcgatctg gtctttttcc aacaatccct tgtcgagggc ggacggggtg atgcagaaca
     2161 gctcctcgtt gatcctcagg ctgatgttgc ccccgctggc ggtggtaagc tgacggtcgt
     2221 agagccggcg catgaactcg gctacgctgg tgcgttcatt cccgaaatga ctgatatcca
     2281 tgagccgagt atatcatgga gagttctcct tgagcacccc cggacgtttt gctacaatgc
     2341 ccttcgtaga ggagaagcta tcgatgagga tgtcacaact cttttcaaaa accctgcgcg
     2401 aagcacccgc cggagcggac agcaaggggt atgaatatct actgcgcgcc ggtttcatcc
     2461 ggcaactcgg ggcgggcatc ttttcccttc tccccttggg ctacagctcc gtcaaaaaga
     2521 tcgagcagat catccgggaa gagatggatg caatcggggg ccaggagatc ctcatgccgg
     2581 tggtcaaccc cgccgatatc tggaaagaaa caggccggtt ctattcgatc gaccgggaaa
     2641 tgagccgctt caaggaccgg gtcggacggg acatggtgct tgcgatgacc cacgaagagg
     2701 tcgtcaccga catcgcccgc ttcgagatcg acagctacaa gcggatgccc cggctcgtct
     2761 accagatcca gacgaaatgg cgggacgatc ccagaccgcg cgccggcctg atccgggttc
     2821 gggagttcac gatgaaggac tcctactcct tcgatatcga ccaagcggga ctggacaagc
     2881 agtacgccgc ccactacctc gcctatttca agatcttcgg ccgcaccgga ctgccgacga
     2941 tcgtcgtcgg tgcggacagc gggatgatgg gtggaaagat ctcccatgaa tacatgtacc
     3001 tctcgccgat cggcgaggat acgatcatca cctgcgactc ctgctcctac accgccaacc
     3061 gtcaggtggc gaccttcagg aaagaacact tccccgaagc gatgaaagag cttgagaagg
     3121 tccatacgcc cgaatgcccg acaattgaag cgttggctga atatttgaag atttcgacga
     3181 gacagaccgc caaggcggtg ttcatgatgg gaacgttcat cgacgacacc aacggcgaag
     3241 aggaacagaa actggtcgtc ggcgtcattc gcggagactt ggagatcgaa gagaacaagc
     3301 tgcagaatgc ggcgaaagcc aacgccctcc gacccgccca cagcgaagag attcttgcag
     3361 cggggatcgt ccccggttac ggctcggtca tcgggatcag gcaggacaag attctcgtca
     3421 tcgccgacga ctccgtcgca aagagcaaca acctcgtcgc cggggcgaat gaagaggggt
     3481 atcactatct gaactccaac ttcacccgcg actggaacgg caccgtcgcc gacatcgcca
     3541 gcgcccaggc gggcttcatc tgcgccgaat gcggagggac gctcaaatca tccaaaggcg
     3601 tcgaggtcgg aaacatcttc cagctgggga cccgctactc cgaatcgatg gactgctact
     3661 atcaggatga aaacggaatc caaaagccgg tcatcatggg cagctacgga atcggggtcg
     3721 gacggctgct tgcctgcctg gccgaagagt accacgatga gctcggattg aacctgccgg
     3781 tcacggtcgc cccgtatcag gttcacctcg tgagccttgt caaggatgcg gagatcgccg
     3841 aagagctcta ccggagactg accgatgccg gcatcaccgt tctgtatgat gatcgcaagg
     3901 aatcggcggg agtgaagttc gccgatgcgg acctgatcgg tcttccgatc cgaatcacct
     3961 tgggcaaccg ctccctgaaa gagggcaagg tcgaggtgaa ggttcgcagc aacccggatg
     4021 aacaatcgag cttcgacctc gcgacccttg ttgacgagat caaagcgctc atcaaacggc
     4081 ttgagaacca aatcaccgat tcaatgtatc tagaagagtg gcaaccggag aagtagcaaa
     4141 gagcgagccc cggttcaggg gctctcttcc tgctctattg ggcgccaagc tttctcagct
     4201 gggtgacgat atacgtccgt ttcgcctcgc gggcgtactc caggacggag tgtccctgat
     4261 aatcccgcgc gttgaaatcc gcccccgcct tcaaaagggc gctgacgatt ctcgtttcag
     4321 gattggtgtt cacggcgatg atgagcgcag tctcgccaag gtagttccgg gcattcaaat
     4381 ccgcccctgc ttcgagcagc gcctcgatca ccttcacatt cgtcgatcgg ttcgccgcca
     4441 agtgcagcgc attggaacga tacttcggct cactctcgtg gatgtccgcc cccgcctcga
     4501 gcagggcttt gagaacaacg actgaagggt tgtattgggc ggccagcatg acgggggtaa
     4561 gaccccagtc gttctgaacg tggatatcag cgccttcttt caaaagatca cgaatctcct
     4621 ttgccttgtc tgcagatgtt gcagcggcaa gaagttttcg attgagtgca tcaattcgtt
     4681 ttgacattgt atacctccta cgcgtatcag tatcccaact tttcattttt ttgtatagca
     4741 cccgataatc cgccccagtg aaggttatgg gtttgctaaa tgggcggaag gtgacgtatc
     4801 atacagtatg ttgaccagcg caatcatcaa acaacttccc aaagtggagc ttcacgacca
     4861 tctcgacggt ggagtccgag tcgaaacaat catcgagctt gcaagcgaat acgggattgc
     4921 tcttccgtcc accgatccca aatccctgca tgaatggttc gcccggggcg gcaagcaaaa
     4981 aagcctcacc ctctaccttc gggcgttcgg cgtcaccaca agcgtgatgc agacaaagga
     5041 agcgttgaag cgaatcgcat tcgaagcggt cgaagatctg gccgaagacc gtgtctgcta
     5101 cgccgagatc cgcttcgccc cgaaccttca taccgccaag ggtctcacga tggaggagag
     5161 cgttcaggcg gtcttggacg gcctcgatca gggaacccgg aaaacagggg ttcccatcgg
     5221 cttgattctc tgtgcgatgc ggaatcaaag ccccgccgtc agcctcgagg tggctcagct
     5281 tgcggtcgcc ttcgccgacc ggggggtcgt cggcttcgac ctcgccggag acgaggccgg
     5341 caatcctccg aagaaacacc ttgatgcgtt tcagttcatt cgctccaaga acttcaacat
     5401 cacccttcac gccggcgaag cgttcggagt ggaatcgatc tggcaggctg tccagatctg
     5461 cggctcccac cggatcggcc acggggtgcg tcttgttgaa gacatgacga ttgaaggatc
     5521 gaagattctt gagatggggt cgcttgcgaa ctacatcctc gatcgccgga ttccgatgga
     5581 gatgtgcctt tcttccaacg tcggaaccgg agcggtcgct tccttgagca aacacccttt
     5641 cccgatcctc ttccgcaacc gcttccgggt attcctctgt gttgacaacc gtctgatgag
     5701 cgatacgacg ctctccaagg aaatggagct cgccgttgaa cattacaatc ttacgatccg
     5761 ggatttggag aagatcacca tcaatgcgat gaagagcgcg ttcatccacc atgaagaacg
     5821 gctgaagatc atctatgatg tgatcaagca aggatacgcc caggttcgta agcagtacga
     5881 aatcctcgac tagagggttc gtctgaacac ctcaaaagga ttgattctca tccggatgcg
     5941 gcagtacagg tacgcgaaag ccaacccgct cagatgcgtc aggtgggcaa cgtttccgcc
     6001 gcggccgaat acctggctgt acagctcgat caacgcatag atgatgatca gaatcggggc
     6061 gcgaaccgga atgattccga agacgaagat caccgaatag ggaaagaaca ccgcaaagag
     6121 caggaggacg ccgtagatcg ctccgctcgc cccgacaagg acgacgttca tcccgccgag
     6181 gtaatagctg ataaaggaga tgattccgct gaacagcccg gtaagcagat agaaaagcag
     6241 gaactccttg ctgccgatcc gcttttccac catcgtcccg aagatcagca agctgagcat
     6301 gttgaagatg aggtggttga acgacccatg gacgaacatg taggtgaaga gctgatagag
     6361 atacccgtgc cggacaaagg atggaatcat cgccagataa tagaatcctt tcggccaaag
     6421 atagtcggta actaaaaaga cgaagacatt gagaatgatg atctttatcg caaagcgtcg
     6481 atatatggtt tccttgaagc ggcggttcag tacggtgggc tttttcatat cgttaatgta
     6541 tcgataggaa tccctatcgt caattgagca ttttttgcac agttggcagg cgttatgcgt
     6601 ccct
//
LOCUS       DAOSLI010000076         6496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_10798_length_6496_cov_21.473218, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000076 DAOSLI010000000
VERSION     DAOSLI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10798_length_6496_cov_21.473218"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..866
                     /locus_tag="PLF67_08410"
     CDS             114..866
                     /locus_tag="PLF67_08410"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOE90070.1"
                     /translation="MKRKLSAKHGVPEHCNGISLRSIIIYRLLKSQDKRPVKEEMLSL
                     PSTYQQDKLEFIKNPVIAEFLGISKDTSYLESDLEQFIIENLQKFIMELGKGYAFVGW
                     QQHSKTEKEDYFIDLVFYNYILTCFVLFDLKTGKITHQDVGQMDMYVRMYDELKRSDG
                     DNPTIGIVLCYDTDEDIARYSVMHGNEQLFAVKYKLYLPTEEELRAEIETRKTLFYLQ
                     QQEKKSENRSCSTPISSTIESIPPPGRRVALR"
     gene            complement(820..1788)
                     /locus_tag="PLF67_08415"
     CDS             complement(820..1788)
                     /locus_tag="PLF67_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE90071.1"
                     /translation="MKYIQLNDTKLARIALGCDHYGEAIPESVANEQLDIYLNSGGNL
                     LDTAHVYGQDTVGGPSSSEQLLGKYLKANRNRKCVMLVSKGCHPPKEDMHASRINHRA
                     MVEDVSRTLENLKTDYLDIWFFHRDDPAVPADEIIDMAQHLIDKKLTKHLGASNWTHR
                     RIAQANAWAKERKKTPFSISQIQWSLAHCTPEQWGDDTLVCMTEEARLWYEEQQMPVM
                     AFSPQAKGFFSKMIAGVGETLSDRAKRRFATDLNLSRIPRVRALAEELGVSPAAVVVA
                     YITSQKNPSIAIAGSSKVEQITDTLNGANLTLTEEQLAFLVGESTQ"
     gene            complement(1788..2666)
                     /locus_tag="PLF67_08420"
     CDS             complement(1788..2666)
                     /locus_tag="PLF67_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOE90072.1"
                     /translation="MKTTTLTPQRMKAILAMQQKEITEYEIYTRIAKRVKDENNKKIL
                     IRIGEEERRHAATWAKVTGKTLKPQMGKVRFYSFLARILGYTFVLKKMEMGEEKAIEG
                     YSHILDEMPQAREIYEDEQKHEEALIAMLDEERLQYVGSMVLGLNDALVELSGTLAGL
                     TFALKNTRLIALSGLITGISATLSMASSEYLSSKNRNEENAGKSAMYTGVAYLIAVAA
                     MVLPYLLLSNYLVALGTMLAVVLLIIVLFTYYTSVAKNLPFGPRFREMAFISIGVSGI
                     SFVIGILVKNWLGIDI"
     gene            2775..3062
                     /locus_tag="PLF67_08425"
     CDS             2775..3062
                     /locus_tag="PLF67_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOE90073.1"
                     /translation="MDESFEDLLKLFAKTTDHDDMRQLLEELLTPNELKDLILRWNLM
                     KDLYRGKPQREIAAAYGISLCKITRGSKILKQKDAYTKRILSDRYDDHLRI"
     gene            3118..4830
                     /locus_tag="PLF67_08430"
     CDS             3118..4830
                     /locus_tag="PLF67_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90074.1"
                     /translation="MSNRRLKRICMILIVLGVFLASIGAEGTILLTRDQVNFYEIVEY
                     SFIVEGKTKPAALRELIVPADGDPIFTSEEKMVASLDSKRQELINTRLFTTVFYEYAF
                     IAYHNGIASYAVTFFIEDSKTLLIFPYPKFSNDRLGLLLGTKVLEKNVLGMLGTLTLT
                     ASIAQNEGGITGWDKRKDTIELDITGLPMIKTKLDLGFSYERIKGSEEGLAKVNTTVS
                     QLKILGTNLAINAHADFAPDSDFTEWNPQTWGLGFTYGPFMQNGGRYTLKSTVEFEGE
                     ELRDIYLTNSITQNNLHFFSLPLLFNIFMDLEIPIDEKIVRSANVSATLGVELPFLFG
                     FRLSASTKPLVEFRNTIDFGAGELTPLSLITAASITRSEIDWVGNFRKGGSLSFSYVR
                     QDYIQANTERDNWHVQGTASWFPFIAFRVNPSIQVAGFYAGGGVKRPFLPSEPQKLGD
                     YFRGFLSSSKALAPFEDADLEWGLITSINLSTDFIDFGFAESYASPFLDIGFFGDSAS
                     EYGYHVVSTIGLEGWAILDRYPAYPARASLGLNLDDLRQAFTKAISWKEVEWELFIGF
                     DLHF"
     gene            complement(4805..5923)
                     /locus_tag="PLF67_08435"
     CDS             complement(4805..5923)
                     /locus_tag="PLF67_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE90075.1"
                     /translation="MTISLLYVDAGKGHYVPAKAMGDVLAARGYDAPVEDLFVALDST
                     FWHWFCKAEWRFFLKHPILERIVHGVSDNRFTNVILRALSKTKPIRTKFIAWYKERKP
                     DVILSTNFLGGVIVGELAAYYKLDVKVYVYAADAFNNPVAGFHINVDKIFIPSFVGEE
                     NLERQGYSQDQIIRSPFPLQQSIEKMERVSKQEARSLLGLEDRFTILYNLGGEGIGSD
                     KLIRYLAKEEADYQIVIVGKTDEQTKKHFLEITKDSKLRVHVPGFVTNIGMYMLACDI
                     QAGKAGANALMESIALRRPFLLSQVLYAARDTTRFFKLRGVGWVEGSSKKQSEIIKTY
                     ARSEEARAEMDSHFDKLPLTFSTEAFVDLLLKNVNQNR"
     gene            complement(5920..>6496)
                     /locus_tag="PLF67_08440"
     CDS             complement(5920..>6496)
                     /locus_tag="PLF67_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOE90076.1"
                     /translation="GTIVTLDGEPHRALHFSNALLHDQQMSIYFSFIFVFLVVLIAFR
                     SFSLSCYALIPVLTGVMANYVIMFALNIPFDLITVSFGAVSVGVGIDYAIHFLLRYRN
                     KIDKTQKQDTAILLKETISETGRPIILTTLSIVAGMLMFLFASYAPVRIFGILMSTAL
                     LNCMLATLFIMPSMIRAVSILKERGLRRRFS"
BASE COUNT         1618 a         1556 c         1656 g         1666 t
ORIGIN      
        1 ttacgattat ttccctcaaa ttttgaacgc agtgagttca aaatctccga tactttcctg
       61 gacgcattat cgctgtttgt tgcaagttcc ggataaagaa gcacgagatt ggtatgaaaa
      121 ggaagctctc agcgaaacat ggagttccag aacactgcaa cggaatatct ctacgcagta
      181 ttattattta tcgtcttctc aaatcgcagg ataagcgtcc ggtaaaggaa gaaatgctgt
      241 ctctgccctc aacgtaccaa caggataagt tggagttcat caaaaatccc gtcattgctg
      301 aattcttagg catttctaaa gacacgagtt atcttgaatc cgatcttgaa cagtttatca
      361 ttgaaaatct acagaagttc ataatggaac tcgggaaagg ctatgcgttt gtcgggtggc
      421 agcaacatag taaaaccgag aaagaggatt acttcattga cttggtcttt tacaattaca
      481 tcctgacatg ctttgtttta tttgatttga aaacagggaa aatcacacat caggatgtcg
      541 gtcaaatgga tatgtatgta cgaatgtatg acgaactgaa acgaagcgat ggtgataatc
      601 ctactatcgg tatcgtcctc tgttatgata cggatgaaga tattgcacga tactcggtaa
      661 tgcatgggaa tgaacagctg tttgcggtga agtacaagct gtatctccct accgaagagg
      721 aactccgtgc agagatagaa acacggaaaa ccttgtttta tcttcaacaa caggaaaaaa
      781 agagcgaaaa caggagctgc tcaactccta tctcttcgac tattgagtcg attcccccac
      841 caggaaggcg agttgctctt cggtgagggt gagattcgct ccgttgaggg tgtcggtgat
      901 ctgctcgacc ttggaggaac cggcgatcgc gatgctcggg ttcttctgac tggttatgta
      961 ggcgaccacc accgctgccg ggctgacacc gagttcttcg gcaagagcac ggacgcgggg
     1021 aattcgactg agattcagat cggtcgcaaa acgtcttttc gcccgatctg agagcgtctc
     1081 tccgacccct gcgatcatct tcgaaaagaa ccccttcgct tggggggaga acgccataac
     1141 gggcatctgt tgttcctcat accaaagacg cgcctcctcg gtcatgcata ccagagtgtc
     1201 atcaccccat tgctcggggg tgcaatgggc gagcgaccac tggatctgac tgatggaaaa
     1261 aggcgttttc ttccgctctt tcgcccatgc gttcgcttgt gcgatccgcc ggtgggtcca
     1321 gttggaggcg ccgaggtgct tggtcaactt cttgtcaata agatgttgag ccatgtcgat
     1381 gatctcgtcg gccggcaccg ccggatcgtc ccggtggaaa aaccagatgt ccagataatc
     1441 cgttttcagg ttctcgaggg ttcggctgac atcctcgacc atcgcccgat gattaatccg
     1501 cgaggcgtgc atatcctctt ttggcgggtg acaccctttg gagacaagca tcacgcactt
     1561 tcgattccgg ttggctttca agtacttgcc cagcagctgt tcgctgcttg aaggtccgcc
     1621 gaccgtatcc tgtccgtaga cgtgggcggt gtccaggagg ttcccgccgg aattgaggta
     1681 aatgtcgagc tgctcgttcg cgaccgattc gggaatcgcc tctccgtaat ggtcacagcc
     1741 gagggcgatc cgggcgagtt tggtgtcgtt cagttggata tatttcatca gatatcaatt
     1801 cccaaccagt tcttcaccag aattccaatg acaaacgaga ttcccgatac tccgattgag
     1861 atgaaagcca tctccctgaa ccgcggtccg aagggaagat tcttcgctac cgaggtataa
     1921 taggtaaaga ggacgatgat caggagaacc accgccagca tcgtgcccaa tgccactaag
     1981 taatttgata aaagcaggta ggggagaacc atcgccgcta cggcgataag gtacgccact
     2041 cccgtataca tagcactttt accggcgttt tcctcgtttc tgttctttga ggagaggtat
     2101 tcacttgagg ccatcgacaa ggtcgcgctg atcccggtga tcaagccgct gagggcgatg
     2161 agccgggtgt tcttgagcgc gaaggttagt ccggccaatg ttccggagag ctcaacaagg
     2221 gcgtcattca gcccaagaac catcgaaccc acatattgaa ggcgctcttc gtcgagcatc
     2281 gctatcagcg cttcttcgtg tttctgctca tcctcataga tctctcgggc ttgaggcatc
     2341 tcgtcaagga tgtgcgagta cccttcgatt gccttttctt cgcccatctc catcttcttc
     2401 aggacgaagg tataccccag gatgcgggca agaaaagagt agaaacgaac ctttcccatt
     2461 tggggcttga gtgtttttcc agtcacttta gcccaggtgg cggcatgccg ccgttcctct
     2521 tcaccaattc ttatcaagat ttttttgtta ttttcatcct taacgcgttt agcgattcga
     2581 gtatatattt catattcggt gatctctttc tgctgcattg caagaattgc tttcatccgc
     2641 tggggggtaa gtgttgtagt cttcatcaat ttctcctctc agtagagtat acgcaccatt
     2701 ggcggagatg gcaaacggtt tgcttgacgg ggcgatattt ctctttgtag aatcaatcat
     2761 tgggaggagt tgttatggat gagagttttg aagatctgtt gaaattgttc gcaaaaacaa
     2821 ccgatcatga tgacatgaga caattactcg aagagctttt gactcccaat gaattgaagg
     2881 atctgatcct ccgatggaat ctgatgaagg acctgtaccg cgggaagccg caacgggaga
     2941 tcgcagcggc gtatgggatc agtctctgca agattactcg gggctcaaag atcctcaaac
     3001 aaaaggatgc gtacaccaaa aggattctta gcgatcggta tgatgaccat ctgaggattt
     3061 aggagagcac tacgaagggc ggaaggaaga ccgatagtgg actgcgggtg gtggactgtg
     3121 agtaaccgcc gactgaagcg aatatgcatg attctcatcg tccttggcgt gttccttgct
     3181 tcaataggag cggaagggac gatccttctc acaagagatc aggtgaactt ctatgaaatt
     3241 gtcgagtatt ccttcatcgt agagggaaaa accaaaccgg ccgccctgcg ggaactcatc
     3301 gtgcctgccg atggcgatcc gatttttacc agtgaagaaa agatggttgc ctcgcttgat
     3361 tccaaacggc aggaactgat aaacacccgg ctttttacca ccgtcttcta cgaatatgcc
     3421 tttattgcct accataatgg aattgcttcc tatgcggtaa ccttcttcat cgaagattca
     3481 aagaccctgc tcatctttcc atatccgaaa ttttccaatg accggctggg gctccttctt
     3541 ggaacgaaag tcttggaaaa aaacgtcctt ggaatgttgg gaactcttac attgacagcc
     3601 agcatcgcac aaaatgaagg aggaattacc ggttgggata agcgaaaaga tactatcgaa
     3661 ctggatatta ccggtttgcc aatgataaaa acaaaactcg atttgggctt ttcctatgag
     3721 cgcatcaagg gcagcgagga aggattggcc aaagtgaata cgaccgtctc gcaactgaaa
     3781 atccttggca ccaatcttgc gatcaatgcg cacgccgatt tcgctccgga ttcggatttc
     3841 actgaatgga atccacaaac atggggattg ggcttcacct atggtccgtt tatgcaaaat
     3901 ggcggacgct acaccttgaa atccaccgtc gagttcgaag gagaggagtt gcgggacatc
     3961 tacctcacca attcaattac tcaaaataac ttgcatttct tcagcctgcc gcttttattc
     4021 aacattttca tggatttgga gattccgatc gacgagaaga tcgttcgttc ggccaacgtc
     4081 agcgccacgt tgggcgttga gttgccgttc ctcttcggat tccgtttatc ggcttcgacg
     4141 aaaccgctgg tggagtttcg aaatacgatt gatttcggtg cgggtgaact gacaccgctt
     4201 tccctgatca ctgccgcctc gatcactcgg agcgagatag actgggttgg aaactttcgc
     4261 aaaggcggtt cgctttcctt ctcgtatgtc cgccaagatt atattcaggc gaataccgaa
     4321 cgggacaact ggcatgttca agggacggcc agctggtttc ctttcatcgc cttccgggtg
     4381 aacccatcga ttcaggttgc cggtttctac gccggtggag gagtaaagcg accattcttg
     4441 ccttcagagc cgcagaagct cggagattat ttccgcggtt ttttgtcttc ctcgaaagcc
     4501 cttgcaccct ttgaagatgc ggatcttgaa tggggtttga ttaccagcat caacctctcc
     4561 accgatttta tcgacttcgg ctttgcagaa tcgtacgcaa gccccttctt ggatatcggc
     4621 ttctttggcg acagcgcatc ggaatacggg tatcacgtgg tcagtacgat cggccttgaa
     4681 ggatgggcta ttctcgaccg gtatcccgcc tatcccgcta gagcgtcttt aggtttgaac
     4741 cttgatgatc tgagacaggc gttcaccaaa gcgatatctt ggaaagaggt tgaatgggag
     4801 ctctttatcg gttttgattt acatttttga gcaaaagatc gacgaacgcc tcagtgctga
     4861 aggtcaatgg aagcttgtcg aaatgggaat ccatctccgc tcgagcctct tccgatcgtg
     4921 cgtacgtctt gattatttca gattgtttct tcgaagagcc ctcgacccaa ccaacacctc
     4981 tgagcttgaa gaaccgagtg gtatcgcgcg ccgcatagag gacttgggaa agaagaaacg
     5041 gtcttctgag ggcgatcgat tccatcaacg cgttcgctcc cgcttttccc gcctgaatgt
     5101 cgcaggcgag catatacatc ccgatgttgg tgacaaaacc cggaacgtgt actcgaagct
     5161 tggagtcttt cgtgatttca agaaagtgtt tcttcgtttg ctcatccgtt ttgccgacga
     5221 tgacgatctg gtaatccgct tcctcttttg cgaggtatct gattaatttg tccgatccta
     5281 ttccttctcc gccgaggtta tacaggattg taaaccggtc ttccaaccca agcagggagc
     5341 gagcctcttg tttggatacc cgttccatct tctcaatgga ttgttgcaaa gggaaaggcg
     5401 aacgaatgat ctgatcttgg gagtatccct gtctctccaa attttcttct ccgacgaaag
     5461 aagggataaa gatcttgtcc acattgatgt gaaagccggc aacagggttg ttgaacgcat
     5521 ccgctgcgta gacatatacc ttcacatcaa gcttgtagta ggcggccagc tccccgacta
     5581 tcaccccgcc gaggaagttc gtcgatagga tcacgtcggg tttgcgttcc ttgtaccagg
     5641 cgatgaattt ggttcggatc ggcttcgttt tggagagagc ccgaagaatg acattggtga
     5701 atcgattgtc cgatacgccg tgaacgatcc gttccagaat gggatgcttc agaaagaagc
     5761 gccactccgc tttacaaaac caatgccaga atgtcgaatc aagagcgaca aaaaggtctt
     5821 caaccggggc atcgtagcct cgagcggcaa gcacatcgcc catcgctttg gccggaacat
     5881 aatgtccctt gcctgcatcg acatacaaga gtgagatggt catgaaaatc tcctacgaag
     5941 tcccctttct tttaggatgc tgaccgcccg gatcatcgag ggcatgatga agagggtggc
     6001 aagcatacag ttcaacaatg cagtcgacat aaggatacca aaaattcgga ccggagcata
     6061 ggatgcgaag aggaacatca acattccggc aacgattgaa agggttgtga gaatgatggg
     6121 ccttccggtt tcactgatcg tctctttcag caggatggcg gtatcctgct tctgtgtttt
     6181 gtcgattttg tttcgatagc gcaacagaaa atgaatcgca taatcgattc cgacaccgac
     6241 ggaaaccgcc ccaaagctga cggtgatcag atcaaagggg atgttgaggg caaacatgat
     6301 gacatagttt gccatcactc cggtgagaac gggaataagg gcgtaacaag aaagggagaa
     6361 ggacctgaaa gcgatgagga cgacgaggaa gacgaagatg aagctgaaat agatcgacat
     6421 ctgctgatca tggaggaggg cgttggagaa atggagcgcc cggtggggtt cgccgtccaa
     6481 ggttacgatc gtgcca
//
LOCUS       DAOSLI010000077         6482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_10823_length_6482_cov_16.873814, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000077 DAOSLI010000000
VERSION     DAOSLI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10823_length_6482_cov_16.873814"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLF67_08445"
     CDS             complement(<1..832)
                     /locus_tag="PLF67_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE90077.1"
                     /translation="MKHEYGTVTAEIVKRLQDIVGASNVLVDRERMEGYSHDETSVEQ
                     YAAMPEVVVTPVTTLQVAAIMKLADAFRIPVTPRGAGSGLSGGAIPIHGGIVLSVEKM
                     DSIVEIDEANLTVTAQAGIVTNELNNRLAELGLFFAGYPMSLETCMLGGNIAENAGGG
                     KAIKYGVTGRYVLGMEVVTPQGEILNLGGKNTKDVSGYDLKQLYIGSEGTLGVITEAT
                     IKLLGLPKTSSDLLVTFTTPQEAISVVPLLMKQGIIPTSIEFMDYLSTRISCDYLNES
                     L"
     gene            complement(829..1824)
                     /locus_tag="PLF67_08450"
     CDS             complement(829..1824)
                     /locus_tag="PLF67_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOE90078.1"
                     /translation="MHDVWTISETLNGRLKRISFELLARGRALADDLGTSLCSIVIGD
                     GISDDEAQKLAHHGADVIYLVNDPSLASFECTRYADVLLSLIKTHKPEVILAGATSSG
                     RTLMPYVGIKAHAGLTADCTELAIDEKTKNLLQTRPAIGGNIMATIITPNHRPQMATV
                     RPRSTKILEPDYGRKAVIQYVKYPDKVSVSTISVTGIRPFEGQQSSIDEAQIIVSGGK
                     GLKKKDNFGMVEELASLLGGEVGATRDAVDRGWISYPHQVGLSGKTVSPKLYLAVGIS
                     GAIQHLAGIKTAECIVAINNDEQANILGLADFAIIGDLFDVVPELARQLAKRKNG"
     gene            complement(1817..2635)
                     /locus_tag="PLF67_08455"
     CDS             complement(1817..2635)
                     /locus_tag="PLF67_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOE90079.1"
                     /translation="MHIVVPIKQVPQSSDVKMDPVTGTMIRSGSASVLNPLDLYAIET
                     ALRLKERWNATVTAVTMGPASAVKILKEALSMGCDEAVHLSAREFSGSDTYATSYILA
                     EAIKKIGNVDLVITGERATDGDTAQVGPGIAAWLEIPVVSYVASLNLVSPSEFTAERL
                     IEEGYQQVQGTLPALLTVVKEIADVRLPTLRGKKRSMDTTIPVWGVREVDPDRSKIGL
                     KGSPTRVVKIETPKVSRSCRTLVIGEELSTEVAVSELVEFLETRSLLGREDDDA"
     gene            complement(2711..3715)
                     /locus_tag="PLF67_08460"
     CDS             complement(2711..3715)
                     /locus_tag="PLF67_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90080.1"
                     /translation="MVISDPALTMNHVEDVFGIRLKYLPFDKERLLPLFLLDAYRIEY
                     AQIDGMKLILLHPRHDLGHIQSIKKHISHIQAIENVPVVLVLPSITRQRRESFMNAGI
                     PFIIQDAQAYLPFLGIKLQERFAVRDVGMLRLQPATQVLFFHYIYSRKTTMFLQEAVH
                     NLGYSAMTISRSITQLAQTNHIIADKEGVRRFFYSPLRGKELFSALEPFLINPVRSRT
                     YMAKKDITEEYLLAGESALTAQSMIEPPPVPCYAGIDSIGGSDELLDSEHQVRVEVWK
                     YDPGILSNSRIVDPLSLYMSYVDERRRQPTLFDISDERVEESLEQVLNNLWERLSYSR
                     "
     gene            complement(3833..5191)
                     /locus_tag="PLF67_08465"
     CDS             complement(3833..5191)
                     /locus_tag="PLF67_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE90081.1"
                     /translation="MIKRLQIKNVGYYRNYSQKGCGGMRIERKQYLDELIKKKDNGRI
                     KIITGIRRCGKSYLLFELYKNYLLKNSIGQNQIIEIALDDIENSQYRDPFKLNEYIKS
                     KTTDDKRYYIFIDEIQFSKSVKNPYIDDDEKITFVDTLLSLMKRKNLDIYVTGSNSKM
                     LSKDILTQFRDRGDEIHMYPLSFAEMSSCYEDKDIAWEDYVLCGGMPFVLTLETFEEK
                     SKYLKRLFEETYIKDIIDRNQIKNSEEVLEVLLDFVSSAVGSLTNPLKLSKRYASEKK
                     IHISNNTISKYLAYFEEAYVLYAAKRYDIKGSKYFSTPLKYYFADIGLRNARLNFRQV
                     EETHSMENIIYNDLLRRGFNVDVGVVEHFPSVDGKTRRVQLEVDFVINRGNLRYYIQS
                     AFAIHDEKKKEQERNSLKRIDDSFKKIIIVKDAIVPRYDEYGIYYIGIRDFLLTDDFL
                     DA"
     gene            5459..6247
                     /locus_tag="PLF67_08470"
     CDS             5459..6247
                     /locus_tag="PLF67_08470"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOE90082.1"
                     /translation="MSLEEITFALVRGGWPDAVTGSKKNALRRAIQYVETIIQTDISS
                     VDNIRKDPVKVRDLMLSLARNTATVATVKTIRDDMVRGDADNALSAPTIYGYLNALER
                     MFVVEDLPAWSPSLRSKTTIRTSPKRHFVDPSIATAVMRLTPDRLLKDFLFFGFLFES
                     LCVRDLRIYADGNDGQVFHYRDANGLEADAVVVLNDGRWGAVEVKLGTKEIEEAAIHL
                     RKLTERINTEKMMEPSFLLILTAGTYAYRREDGVLVVPLACLKD"
BASE COUNT         1522 a         1618 c         1585 g         1757 t
ORIGIN      
        1 gaaggctttc attgaggtag tcacaggaga ttctggtgga gaggtagtcc atgaactcga
       61 tcgaggtggg gatgattccc tgcttcatca acagggggac gacggagatc gcctcctgcg
      121 gggtagtgaa ggtgacaagc aggtccgagg aggttttcgg caagcccaga agcttgatcg
      181 tcgcctcggt gatgaccccc agcgttccct ccgatccgat gtagagctgc ttcaggtcgt
      241 agccgctcac gtctttggtg ttttttccgc ccagattcag gatctctccc tgcggcgtca
      301 cgacctccat gccgaggaca taccggccgg tgaccccgta cttgatcgcc ttccccccgc
      361 cggcgttctc ggcaatgttt cccccgagca tgcatgtttc aaggctcatc ggatagccgg
      421 cgaagaagag acccagctcc gcgaggcggt tgttgagttc gttcgtgacg atccccgcct
      481 gggcggtcac cgtgaggttc gcttcgtcga tctccacgat cgagtccatc ttctccacgg
      541 agaggacgat tcccccgtgg atcgggatcg ccccgccgga gagccccgat ccggctccgc
      601 ggggcgttac cggaatcctg aacgcatcgg cgagcttcat gatggccgcc acctgaaggg
      661 tcgtcaccgg ggtcacgacg acctccggca tcgcggcata ttgctcgacg gaggtctcat
      721 cgtggctgta gccttccatc cgctcccggt cgaccaggac gttggacgcc ccgacgatat
      781 cctgcagccg cttcacgatc tccgccgtga cggttccata ctcatgcttc atccgttctt
      841 cctttttgca agctgccggg caagctccgg caccacgtcg aagaggtcgc cgatgatcgc
      901 gaagtcggcg agtcccagaa tatttgcctg ttcatcgttg ttgatcgcga cgatgcactc
      961 cgcggtcttg atgcccgcca gatgctggat cgccccggag attcccacgg cgaggtagag
     1021 cttcggcgag acagtctttc cggaaagccc gacctggtgg gggtaggaga tccacccccg
     1081 atcgacggcg tcgcgggtgg cgccgacctc gccgcccaga agggaagcaa gctcttcgac
     1141 cattccgaag ttgtccttct tcttgagacc tttcccgccg gaaacgatga tctgtgcttc
     1201 atcgatgctg ctctgctgcc cttcaaaggg tcttataccg gtaactgata ttgtgcttac
     1261 tgaaacctta tcgggatact taacatactg tattaccgct tttcgaccat agtccggctc
     1321 caagatcttc gtggagcggg ggcggaccgt cgccatctgc gggcggtggt tcggggtgat
     1381 gatcgtcgcc atgatgttgc cgccgatggc gggacgggtc tgcagcaggt tcttcgtctt
     1441 ctcatcgatc gccagctcgg tgcagtccgc agtcaggccc gcatgggctt tgatgccgac
     1501 atacggcatc aatgttcgac cactgctcgt cgcaccggcg aggatgacct cgggtttgtg
     1561 ggtcttgatc agggagagga ggacatcggc atatcgggtg cactcgaacg aggccagaga
     1621 agggtcgttc accagataga tgacgtccgc tccgtggtgg gcgagttttt gcgcctcgtc
     1681 gtcgctgatt ccatccccga tgacgatgct gcagagggag gtcccgaggt catcggcaag
     1741 ggctcttccc cgggccagca gctcgaagga gatccgtttg agtctaccat tcagcgtttc
     1801 actgatcgtc cagacatcat gcatcgtcat cctcccgacc aagcaggcta cgagtttcca
     1861 gaaactccac caattccgag actgcgactt cggtgctcag ctcttcgccg atgacgagcg
     1921 tcctgcagga tcggctcacc ttgggcgtct cgatcttgac cacccgggtc ggcgagcctt
     1981 tcaatccgat cttcgaacgg tcggggtcga cttcccggac cccccagacc ggaatggtcg
     2041 tgtccatcga gcgcttcttc ccgcgcaggg tcggcagccg cacgtccgcg atctccttca
     2101 cgacggtgag cagggcgggg agggtgcctt ggacctgctg atatccttct tcaatgaggc
     2161 gttcggcggt gaactccgag ggagagacaa ggttgaggga cgcgacgtag gagacgaccg
     2221 ggatctcaag ccaggcggcg atccccggcc ccacctgagc cgtatctccg tcggtcgccc
     2281 gctcgccggt aatgaccaga tccacgttcc cgatcttttt aatcgcttcg gcgaggatgt
     2341 acgaggtcgc ataggtgtcg gatccggaaa actcccgggc gctcagatga accgcttcat
     2401 cgcagcccat gctgagggct tccttcagaa tcttcacggc gctcgccggc cccattgtga
     2461 ccgccgtaac ggtcgcattc caccgttcct tgagcctcag cgccgtttcg atcgcataga
     2521 gatccagggg attcagcacc gaggccgatc cgcttcggat catcgttcca gtaaccggat
     2581 ccatcttgac gtcgctgctc tggggaacct gtttgatcgg tacgacgata tgcatggccc
     2641 ctcctgttga ggacagtcta gaacgagaag gcgcacttgg caagaaaaat gaccgcggtc
     2701 aattatccta ctacctcgaa tatgacaacc gctcccaaag attgttcaaa acctgttcca
     2761 gcgattcttc gacgcgttca tccgaaatgt cgaagagtgt cggctgtcgt cttcgctcgt
     2821 caacgtacga catatataat gacagcggat cgacaatgcg actattgctg agaatgccgg
     2881 gatcatattt ccatacttcg acacggactt gatgctcgga atcaagaagc tcatcggatc
     2941 cgccaatcga gtcgattccc gcataacagg gcaccggagg cggttctatc attgattggg
     3001 ctgtgagtgc ggattctccg gcaagcagat actcttccgt gatatccttc ttggccatat
     3061 atgttcggct tcgaaccgga ttgatcagaa aaggctcaag tgccgaaaag agttctttgc
     3121 cccggagcgg agaataaaaa aatctgcgta ctccctcttt atcggcaatg atatggttgg
     3181 tttgagcaag ttgagtgatt gaacgcgaga tggtcattgc tgaatatccg agattgtgca
     3241 cggcttcttg gagaaacatg gttgttttcc tgctgtatat gtagtgaaag aacaatacct
     3301 gagttgccgg ttgcagtctt aacattccaa catcccgaac ggcaaaccgt tcttggagct
     3361 tgattcctaa aaatggtaag tatgcttgag cgtcctgtat gatgaaaggg attccggcgt
     3421 tcataaacga ttcccgtctt tgtcgagtaa ttgaaggcag gacaagaacc accggaacat
     3481 tctctatggc ttggatgtga gagatatgtt ttttaatgga ttgaatgtga cccagatcat
     3541 gacgagggtg aagcaggatt agcttcattc catcgatttg agcatattca atacgatatg
     3601 catccaagag aaacaaagga agcagtcgtt ccttatcgaa aggaagatac ttcaacctta
     3661 ttccgaatac atcctctaca tggttcattg tcagcgccgg gtcagaaatc accacatagt
     3721 gccaggttac attttgccag ttagcaacgg attgaaattt gccaccccta cccgaactct
     3781 ttcattattt caatggcaag attgttatct tgaatagttg aaggttattc aactatgcgt
     3841 caagaaaatc atccgttaac aaaaagtctc tgattccaat gtagtaaatt ccatactcat
     3901 cgtatcttgg cactatagca tctttgacaa taatgatttt cttaaaagaa tcatcaattc
     3961 gttttagaga gtttctttct tgttctttct tcttctcatc atgtatagcg aaggctgatt
     4021 gaatataata tctgagattt cctctgttaa tgacaaaatc aacctctaac tgcactcttc
     4081 ttgttttccc atctacacta ggaaaatgtt ccacaacgcc tacatctaca ttaaatcctc
     4141 ttcttagcaa gtcgttataa attatatttt ccatactatg cgtttcttcc acttgtctaa
     4201 aattcagtct tgcatttcta agcccaatgt cagcaaaata atatttaagt ggtgtagaaa
     4261 agtattttga acccttaata tcatatcttt tagctgcata taaaacatag gcttcttcaa
     4321 aataagctaa atactttgaa atggtgttat ttgatatatg tatctttttt tctgaagcat
     4381 atcttttgga taacttaagg ggatttgtaa gcgatcctac cgctgatgat acaaaatcca
     4441 ataaaacttc caatacttct tcactgtttt ttatctggtt tcgatctatg atatctttga
     4501 tgtacgtttc ttcaaacagt ctttttaaat acttgctttt ttcttcgaag gtttcaagtg
     4561 taagtacaaa tggcattcca ccacaaagca cataatcttc ccatgcgata tctttatcct
     4621 catagcagct actcatttca gcaaacgata aaggatacat atggatttca tctcccctat
     4681 ctctaaattg tgtcaaaata tctttagaaa gcatttttga attgcttccg gtaacgtata
     4741 tatcaagatt tttccgtttc ataagcgaaa gtaacgtatc aacaaaagta atcttttcgt
     4801 catcatcaat atatggattc ttcaccgact ttgaaaattg tatctcatca ataaaaatgt
     4861 aatacctctt atcatcagtt gttttgcttt ttatatactc attgagttta aatggatccc
     4921 tatactgact attttcgatg tcgtcgagag caatttcaat aatttggttt tgacctatac
     4981 tgtttttcaa taaataattc ttgtatagtt caaacaacaa gtatgacttg ccacatcttc
     5041 tgatacccgt aatgatcttg attcttccgt tatctttttt cttgatcaac tcatctaaat
     5101 attgttttct ttctatacgc ataccaccac atcctttttg tgaataattt ctgtagtatc
     5161 ccacattttt tatctgtaat cgttttatca cattcaaaag agaaattcaa ctactaggaa
     5221 taatttccgt ggcataacac actttttatt ccatcctaca gcgtctatag ttgctatctc
     5281 ttctttgata tgccgtgagg tttgccgtgg ataaacgggg aggaaccggc catttcattc
     5341 ttacgggctc tgcggtgcct cctgccgagg agaccccggg tcggggagaa tttctcgttt
     5401 caacaagagg cagagagatc tctttgatgg aaaggaagat atttcgagca gatcatcaat
     5461 gtctttggag gagatcacct tcgccttggt gcgaggtgga tggcctgatg cggtgacagg
     5521 atcgaaaaaa aacgctttac ggcgagcgat ccagtatgtt gaaacaatca tacagactga
     5581 tatttcaagc gttgacaata tcagaaaaga tccggtgaaa gttcgggatc tgatgctctc
     5641 cctcgccaga aacaccgcca ccgtggcgac cgtcaagacg atccgggatg atatggttcg
     5701 aggagatgcc gacaatgccc tctccgctcc aacgatctat ggatatctca atgcgcttga
     5761 acggatgttc gtcgtagagg atctgccggc atggagccct tcattgcgtt ctaaaacaac
     5821 catcagaaca tcaccgaagc gtcattttgt cgatccctcg atcgcgactg ccgtgatgcg
     5881 tttgactccc gataggttgc tgaaggactt cctgtttttt gggtttctct tcgagtcttt
     5941 gtgtgttcgg gatcttcgaa tatatgcgga tggaaacgat ggacaggtat ttcactatcg
     6001 ggatgcgaat ggtctggaag ctgatgccgt ggtggttctt aatgatggca gatggggggc
     6061 cgttgaggtg aaactcggta caaaggaaat agaagaggcg gctatccatt tgcgtaagct
     6121 tacggaacgg atcaataccg agaagatgat ggagccttcc tttttgttga tactgactgc
     6181 aggcacgtat gcctatcgtc gggaagacgg agtgttggtg gttccccttg catgtttgaa
     6241 ggattgatat gtgatcatgg aaagaaccga tggagtacgc tttcaaaaaa caaaaaccat
     6301 tgaagtttcg aaagcgtaca tcccggttcg tgtaggataa catcgctcat ggtatcgtga
     6361 tgaaaacaat gaggatgcct ccccgccgtc tgcggggagg tatctggtct cctatttgaa
     6421 gaacgtatgg cggagccagc gctgggaagc gactgcgtcc cctttggtga caagttcgcg
     6481 ga
//
LOCUS       DAOSLI010000078         6456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_10864_length_6456_cov_29.191220, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000078 DAOSLI010000000
VERSION     DAOSLI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10864_length_6456_cov_29.191220"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..279)
                     /locus_tag="PLF67_08475"
     tRNA            complement(207..279)
                     /locus_tag="PLF67_08475"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Phe,seq:gaa)
     gene            377..1582
                     /locus_tag="PLF67_08480"
     CDS             377..1582
                     /locus_tag="PLF67_08480"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOE90083.1"
                     /translation="MESLRELYRIGHGPSSSHTMGPRSAGEHFLQQYPHAASFVAELY
                     GSLAATGKGHLTDTTLREVFAKGEKPIEILWYREVEQKHPNTLVIKAFDEEDRIIASR
                     TYYSVGGGKIVIEGEENPASVSVYPPELTKMSAILEYCNKEGLQLWEFVLEVEGKEIL
                     TYMKEVWGVMSDSVERGIEEEGVLPGGLKLPRKAHSYFLKASKGAYPLDDSALALSYA
                     LAVSEENASGNLVVTAPTCGSAGVLPGVLYYYAKQKNAGRIRILRALLTAGLVGNLVK
                     TNGSISGAEVGCQGEIGVACAMAGAAAAQLNGGSIFQVEYAAEMGLEHHLGLTCDPML
                     GLVQIPCIERNALGAMRSLDHATYALLGDGRHKISFDTVVEVMLETGQALPSLYRETS
                     LGGLARVRS"
     gene            1616..2368
                     /locus_tag="PLF67_08485"
     CDS             1616..2368
                     /locus_tag="PLF67_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE90084.1"
                     /translation="MNDELLRVKGLFYSLGEDKTMLSNLSFSIQEGSFTLLCGPNGAG
                     KSTLLRILKGLYPLEAGTIVLEGKDVTKREKDRLRKIGLVFQDADSQFVGQSVEKDIR
                     FGLENLNLPLEEQNRRIDEVLHLLSMKEQRHRHPHCLSGGEKRKLAIAGVLAMQPKLI
                     LLDEPFANLDYRSTGATLKLLLRLQKEGHTIILVSHEIEKCMAHADQVLVLNDGLLVE
                     NGSPETVLPRLPEHGIHVPHDTLLEELTWLAQ"
     gene            2353..3036
                     /locus_tag="PLF67_08490"
     CDS             2353..3036
                     /locus_tag="PLF67_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90085.1"
                     /translation="MARTVIFSYRHQDRLLNRTHPFIKVLALLCGCILLFNASVFLVS
                     IIALFLVVLTGIQRLPLRSYARQLRFFILLFLLIALGEYLRSHSVSSALIVLARYALM
                     ILYALLITDSTSSDEIASSIPLVNKSVLAGGIELTLAIVPMVFQVSEEVRIAQRARLR
                     RWSTKGTFGYLSTLFELLLIRLEERALALEGRLFDAKRKRHAPPLSLYDLPMVLATAL
                     FIGLRLVRP"
     gene            3033..3578
                     /locus_tag="PLF67_08495"
     CDS             3033..3578
                     /locus_tag="PLF67_08495"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOE90086.1"
                     /translation="MNKEEIRKAMRKLNQAMQSVDHGAEDMATVRALVNSPLFQKSDW
                     VFGFMPLYCEVDIAPVLQAALQTRRLALPRSNGDDTLTFVEVKSLDELKPGRFGILEP
                     KAGVEAQPTSASLIIVPALAYTPKRVRLGRGKGFYDRYLERFPDPVSIGVCRTWQLIE
                     ELPVSIYDRQVDSLLCNGTWY"
     gene            complement(3579..5066)
                     /gene="gltA"
                     /locus_tag="PLF67_08500"
     CDS             complement(3579..5066)
                     /gene="gltA"
                     /locus_tag="PLF67_08500"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOE90087.1"
                     /translation="MHPKKQLLDDAAKLLLAELEGKELTARERNQIPLQEMATQDPSE
                     RILNMNEVALGYTEAQVRIEAMRCLQCKTKPCVKGCPVGIDIPAFILEAEKGNYAKAV
                     SIIKESSLLPSVCGRVCPQELQCQMYCTVGKAKKDVEQSVSIGRIERFVADYTREHGL
                     DEIPHVGDDSGKSVAIVGSGPASISAAADLRREGHRVVLFEALHKAGGVLVYGIPEFR
                     LPKEIVEYELEILRKMGVEIRLNYLVGKTRTVDELFDKDGFDAIFVGSGAGLPRFLDI
                     EGENSIGVFSANEYLTRSNLMKAYAKTNALTPMYRSKRVAVFGAGNVAMDAARTAKRL
                     GAEVVSIIYRRTETEMPARREEIAHAKEEGIEFLYLLAPKRIIPGEDGRVKEVEVIEC
                     KLGPEDEDGRRRSMMIEGTESLRSYDTVIVAIGNDSNPLIKATTDGIETSHKGTFIVD
                     PSTCATSLEGVYAGGDIVLGAATVILAMGQGRKAARAMNEYLATL"
     gene            complement(5069..5917)
                     /locus_tag="PLF67_08505"
     CDS             complement(5069..5917)
                     /locus_tag="PLF67_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOE90088.1"
                     /translation="MNKIIAKKQLSATVFQMEIEAPEIAEARKAGQFIILQLGGEFDE
                     RIPLTIADADPVKGTITIIFQAVGESTHHIALLKEGDYIENLLGPLGNPTDVRHYGHV
                     VCTGGGIGAAPLFPIVEAMKAAGNRVTVILGARTKELVILEEKMRAVADEVIIVTDDG
                     SYGEKALVTEPLKRICETTQVDCVVAIGPPVMMKFVSLTTKPFGIYTIVSLNTIMIDG
                     TGMCGGCRVTVDGRTRFVCVNGPEFDGHLVDWDNLLLRMGTFRAQEEEAHHRCLVGLD
                     TKEGEA"
     gene            6036..6380
                     /locus_tag="PLF67_08510"
     CDS             6036..6380
                     /locus_tag="PLF67_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE90089.1"
                     /translation="MIKRKNEMNTEVRSKMRGGSGEARFTYLMNANEVPHARLFSTIT
                     LDPGSSIGEHTHTNEIEYYWILQGEGVVTEQDGEKVVREGDLVITGGGASHAIRNEGT
                     TPLRFLALIILE"
BASE COUNT         1343 a         1918 c         1748 g         1447 t
ORIGIN      
        1 ttttagtagt agtttactag tagttttttc ttcatcactc ctatcaaaac cttcattctt
       61 ccataaaatt ccgcattcac cctcgtaacc gtattggtgg agatcacgtg tctcctccct
      121 ctcaatgata gccaaggaac agggaaaaag caaaagaagt tagcatgaaa gaacccctcg
      181 ctcccgaagg aacaaggggt atgaggtgct acctctcaga gttgaactga ggacacatgg
      241 attttcagtc cattgctcta ccaactgagc taaggtagct aacgttcatt tgtatacccc
      301 acatcttcaa ttcttgtcaa cacccggtga gaaaatacgc tttcattttg agggtgaaaa
      361 ggctatgatc gagggtatgg aaagtcttcg ggaactctat cggatcgggc acgggccatc
      421 ctccagtcac acgatgggtc cgcgcagcgc cggcgagcat tttttgcagc aataccccca
      481 tgccgcctcg tttgtcgccg agctctacgg ctccctggcc gccacgggaa aggggcacct
      541 caccgatacc acccttcggg aagtctttgc gaaaggggaa aaaccgattg agattctgtg
      601 gtatcgggaa gtggaacaga aacacccgaa caccctcgtc atcaaggcgt tcgatgaaga
      661 agaccggatc atcgcttccc ggacctacta ctccgtcggc ggagggaaga tcgtcatcga
      721 aggggaggag aatcccgctt cggtttcggt ctacccgccc gaactgacga agatgagcgc
      781 gatcctcgag tactgcaaca aggaaggact tcaactgtgg gagttcgtcc ttgaggttga
      841 aggcaaagag attctcacct acatgaaaga ggtctggggc gtgatgagcg acagcgtcga
      901 gcgggggatc gaggaggagg gggttcttcc cggcggactg aagcttcccc gaaaagccca
      961 cagctacttc ctcaaggcga gcaaaggcgc gtatcctctg gatgattcgg ccctcgctct
     1021 ctcctacgct cttgccgtca gtgaagagaa cgcctcgggc aacctggtcg tcaccgctcc
     1081 tacctgcggt agcgccgggg tgcttcccgg agtgctctac tactacgcca agcagaagaa
     1141 tgccggaagg attcggattc ttcgagccct gcttaccgcc ggactggtcg gaaacctcgt
     1201 gaaaacaaac gggtcgatca gcggggctga agtcggttgc caaggcgaaa tcggagtggc
     1261 gtgcgcgatg gccggggccg ccgccgccca attgaacggc ggatcgatct ttcaggtcga
     1321 gtacgctgcg gagatgggct tggaacacca cctcggactg acctgcgacc cgatgctggg
     1381 attggtccag atcccctgta tcgaacgcaa cgccctcggg gcgatgcgct ccctcgacca
     1441 cgccacttat gctctgctcg gggacggccg gcataagatc agtttcgaca ccgtggtcga
     1501 agtgatgctg gaaacgggtc aggcccttcc ctccctctac cgggagacga gcctcggcgg
     1561 attggcccgg gtccggagtt gacaaaccaa acccataacg gacaccctta tgcccatgaa
     1621 cgacgaactg cttcgggtta aaggactctt ctattccctt ggcgaagata aaaccatgct
     1681 cagcaacctc tccttctcca tccaagaagg aagcttcacg ctcctgtgcg gtcccaacgg
     1741 ggcgggcaaa agcaccctcc ttcgaatcct caaaggcctc tatcccctcg aagcgggaac
     1801 gatcgttctt gaaggcaagg atgtaaccaa aagagaaaaa gaccggcttc ggaagatcgg
     1861 cctcgtcttt caggacgccg acagtcaatt cgtcggccag agcgtggaaa aagacatccg
     1921 cttcgggttg gaaaacttga atcttccatt ggaggagcaa aaccggcgga tcgatgaagt
     1981 gctgcacctc ctttccatga aagagcagcg ccaccggcac ccccactgct taagcggcgg
     2041 tgaaaaacga aagctggcga tcgccggagt cctggcgatg cagccgaagc tcatcctcct
     2101 cgacgaaccc tttgcgaacc tcgattaccg ctcgaccggg gcgaccctca agctcctgct
     2161 ccgcctgcaa aaagagggac atacgatcat cctcgtcagc catgaaatcg aaaaatgtat
     2221 ggcccatgcc gatcaggtgc tggtcttgaa tgatggcctg ctcgttgaga atggaagtcc
     2281 tgaaacggtt cttccccgcc ttccggagca tggaatccat gtaccgcatg acaccctgct
     2341 ggaggagctc acatggctcg cacagtgatc ttctcctacc gccatcagga tcggcttctc
     2401 aacaggacgc acccgttcat caaggtgctc gctctcttat gtggctgcat cctgcttttc
     2461 aacgcatccg tttttcttgt ttcgatcatc gcgttgtttc ttgtcgttct gacgggtatt
     2521 caacggcttc ccctccgctc gtatgctcgt caactcaggt ttttcatcct tttgttcctc
     2581 ctcatcgccc tcggcgaata ccttcgctcg cactcggtct cctcggctct gatcgtcctt
     2641 gcccgctacg ccttgatgat tctctatgcg cttctgatca ccgattccac aagcagcgac
     2701 gagatcgcaa gctccatccc cctggtcaac aagagtgtct tggccggcgg gatcgaactg
     2761 accttggcga tcgttccgat ggtcttccag gtcagcgagg aagtccgcat cgcccagcgg
     2821 gcgcgcctgc gacgatggtc gactaaaggc acgttcgggt atctctccac gctgttcgag
     2881 cttcttctga ttcggctcga agaacgggcc cttgccctcg aaggccgcct cttcgatgcg
     2941 aagcgcaagc gacacgctcc gcctctatcg ctctatgacc ttccgatggt acttgccacg
     3001 gcccttttta tcggtctgag gttggttcgg ccatgaataa agaagaaatc cgcaaagcga
     3061 tgaggaaatt gaatcaggcg atgcaaagcg tcgaccatgg tgcggaggat atggcgacgg
     3121 tgagggcgct ggtgaactcc ccgctctttc aaaaaagcga ttgggtcttc ggcttcatgc
     3181 cgctttattg cgaggtggat attgcacccg ttttgcaagc ggctcttcag acaaggcgct
     3241 tggcgcttcc ccgctccaat ggcgatgaca ccctcacctt cgtggaagtg aagagtcttg
     3301 atgaactgaa gccggggcgc ttcgggatcc ttgaaccgaa agcaggtgtt gaggctcagc
     3361 ccacgtcagc ctccctgatc atcgtcccgg ctttggcgta tacccccaag cgagtgcgcc
     3421 tcggccgggg aaagggattt tacgaccggt atctggagcg ctttcccgac ccggtgagca
     3481 tcggcgtctg tcgaacctgg cagctgatcg aagaactgcc ggtcagcatc tatgaccggc
     3541 aggttgattc cttgctctgc aacggaacgt ggtattgatt acagagtcgc caagtattcg
     3601 ttcatcgctc ttgcggcttt tcttccctgg cccatcgcca gaatgaccgt ggccgccccg
     3661 agaacgatgt ctccgcccgc atacacccct tccaggctgg tagcgcacgt cgaaggatcc
     3721 acgatgaatg ttcccttgtg gctggtttca atgccgtccg tcgtcgcttt gatgaggggg
     3781 ttggaatcat ttccgattgc gacgatcacg gtgtcgtacg atcggaggga ctccgtcccc
     3841 tcgatcatca tcgagcgccg gcgcccatcc tcatcttccg gtcccagctt gcattcgatg
     3901 acctcgacct ctttcacccg gccatcctcg ccgggaatga tccgcttggg ggcaaggagg
     3961 tagaggaact cgatcccctc ctctttggcg tgggcgatct cttcccgccg ggcgggcatc
     4021 tcagtctccg tccggcggta gatgatgctc accacctccg ctccgagacg cttggcggtt
     4081 cgggcggcat ccatcgcaac gttgcccgct ccgaagaccg ccactcgttt ggagcgatac
     4141 atcggtgtca acgcgttggt cttggcgtag gctttcatca ggttgctgcg agtcaggtat
     4201 tcattggcgc tgaacacccc gatcgagttc tccccctcga tatccaggaa tcgggggaga
     4261 cccgctccgc tgccgacgaa gatcgcatcg aagccgtcct tgtcaaagag ttcatccacc
     4321 gtgcgggtct ttccgacaag gtagttcaat cggatctcca cccccatctt ccgaaggatt
     4381 tccagctcgt attcgacgat ctccttcggg aggcggaact cgggaatccc gtagaccaga
     4441 acgcctcccg ctttgtgcag cgcttcaaac agcacaaccc ggtgcccctc ccggcgtaaa
     4501 tccgctgcgg cggatatgct ggccggaccg cttccgacga tcgccaccga cttgccactg
     4561 tcatccccga cgtgagggat ctcatcaaga ccgtgctccc gggtgtaatc ggcgacgaaa
     4621 cgctcgatcc gcccgatcga aacggattgc tccacatcct tttttgcctt ccccacggtg
     4681 cagtacatct ggcactggag ctcctgggga cagacccgtc cgcagacgga gggaagcaga
     4741 cttgattcct tgatgatgga aaccgccttt gcatagtttc ccttctccgc ttccaggatg
     4801 aaggcgggga tatcgatgcc gacggggcat cccttgacgc agggcttggt tttacactgg
     4861 aggcagcgca tcgcttcgat gcggacttgg gcttcggtat acccgagggc gacctcgttc
     4921 atattgagga ttcgttcgct cgggtcctga gtggccatct cctgcagggg aatctgattg
     4981 cgctctctgg ccgtgagctc ttttccctcc agctcggcga gcagcagttt cgcggcgtca
     5041 tcaagtagtt gtttcttcgg gtgcatctct acgcctctcc ttctttggta tccaagccga
     5101 cgagacagcg gtggtgcgcc tcttcttcct gagccctgaa ggtgcccatc cgaagcagaa
     5161 ggttgtccca atcgacaagg tgtccgtcaa actcaggacc gttcacgcac acgaatcggg
     5221 tgcgcccgtc gaccgttacg cggcatcctc cgcacattcc cgtgccgtcg atcatgatcg
     5281 tgttcaaact gacgatcgta tagattccaa agggcttggt ggtcagcgag acgaacttca
     5341 tcatcaccgg aggtccgatc gccacgacgc aatcgacttg ggtcgtttcg cagattcgct
     5401 tcagcggctc ggtcaccagc gccttctccc cgtatgaacc gtcatcggtt acaatgatca
     5461 cttcatcggc gacggcgcgc atcttctctt ccagaatcac caactctttc gtccgggctc
     5521 cgagaatcac cgttaccctg ttgcccgccg ctttcatcgc ctcgacgatg ggaaagagcg
     5581 gagccgctcc gattcctccg ccggtgcaca ccacatggcc gtagtggcgg acgtcggtgg
     5641 ggttgccgag cggtccgagg aggttttcga tgtaatcgcc ctcctttagg agcgcaatat
     5701 ggtgggtcga ttcgccgacc gcctgaaaga tgatggtgat tgtccccttg acgggatcgg
     5761 catcggcgat cgtaagaggg atccgttcat caaactctcc gccgagctgg agaatgatga
     5821 actggccggc ctttcgcgct tcagcgattt caggagcttc aatctccatc tgaaacactg
     5881 tagcagacaa ctgttttttc gcaatgattt tgttcacacc cgcttcctcc gatacctcat
     5941 tcgtcccatg aaaaggatac aatagtattg aaagtggcag cattgttgtc aagtcatccc
     6001 ttggcaaacc cccgctgcgg tggcacactg ttcatatgat taaacgaaaa aatgaaatga
     6061 acaccgaagt tcggtccaag atgagagggg gaagcggcga ggcccgcttc acctacctga
     6121 tgaatgcaaa cgaggtcccg cacgcccggt tgttttcgac gatcacgctg gatcccggct
     6181 cctcgatcgg cgagcacacc cacacgaacg agatcgagta ctactggatt ctccaaggcg
     6241 aaggtgtcgt caccgagcag gacggagaaa aggtcgtcag ggaaggtgat ctggtgatta
     6301 ccggcggtgg agcgagtcat gcgatccgca atgaaggaac aacgccgctg cgcttcctcg
     6361 ccttgatcat cctcgagtag ggagaaatgg tttcaggcag cctcccggct gcctgaatgt
     6421 acaaagctga tccgtttttt cttacttgat cttcac
//
LOCUS       DAOSLI010000079         6369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_11049_length_6369_cov_24.553532, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000079 DAOSLI010000000
VERSION     DAOSLI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11049_length_6369_cov_24.553532"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLF67_08515"
     CDS             complement(<1..709)
                     /locus_tag="PLF67_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE90090.1"
                     /translation="MATLILALVSLTIGSSRIGLSETIGVFTGRNTSSAQRYIILNLR
                     LVRIIASFAAGTILALGGVSYQAVLRNPMADPFILGVSSGASFGVALAIYFGVATRFG
                     LPLSALLGATGTTLFILALASRKRSSNTVLLLSGVAANYILSAAMTLLLFLNREQYQR
                     ILAWSLGTFSTATWNQVVIAFLVSVLLLLFLIGNHRNLDLLLLDEGSARSSGLAVESV
                     RYRLLLISSFATALCVSF"
     gene            complement(741..1595)
                     /locus_tag="PLF67_08520"
     CDS             complement(741..1595)
                     /locus_tag="PLF67_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE90091.1"
                     /translation="MKRHFLTPLFIVISMIVLMLAPLSAQGMMEQPVEQRVVSLSPNV
                     TETIYALGGGDLLVGRSDYCNYPAEADSLPSVGTLYNPSLEMILALEPTHVISSAFVP
                     DELLFSIEQANINVLSISAQETFEGTYDLIRAVGDVIHRQSQAEQLIGEMKAQVKAIE
                     EKAAPLSKVTVYMALDFGSFDSAATGDTFLHEMIEKAGGTNVAADGRYWTYSKELLVE
                     KEPQLILLSPRWGESEEATIREFTTTKPYSDLKATVKGFDADVVLRQGPRSAQALQIL
                     FDLIDGER"
     regulatory      complement(1644..1812)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1863..2315
                     /gene="rnhA"
                     /locus_tag="PLF67_08525"
     CDS             1863..2315
                     /gene="rnhA"
                     /locus_tag="PLF67_08525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOE90092.1"
                     /translation="MMSYHEMTIYTDGGCIGNPGVGAWAYVLEADGVFVKEGSGYDPE
                     TTNNKMELTAVIEALKAASALGARKITLYTDSQYVKNGITSWINKWKINGWRTAGRDP
                     VKNKEYWVALDELRNLADVTFSWVKGHAGIPGNERCDALVAQTIEAHQ"
     gene            2312..2866
                     /locus_tag="PLF67_08530"
     CDS             2312..2866
                     /locus_tag="PLF67_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOE90093.1"
                     /translation="MKKTSLWDLYFTFFKIGGLTFGGGYAMLPLLQEEVVNKRRWASE
                     EELLDMYAIGQVTPGIIAVNTATMVGYRKRGILGGIIATLGEISPSILIITTLATILL
                     RYKDAVYLQQAFVGIRIAVCATVTVSIIKLMKKSLVDLPTVLFYLLVVASTLMFSLSP
                     IIVVIVAILFGIVVQNIKHKKERT"
     gene            2863..3408
                     /locus_tag="PLF67_08535"
     CDS             2863..3408
                     /locus_tag="PLF67_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOE90094.1"
                     /translation="MIYLTLFYEFFKIGLFSIGGGLATLPFLYKLAEKRPDWLTIQTI
                     ADMIAVSESTPGPIGINMATYAGYQVAGIFGGIIATLGEVAPSIIIIVIIAKFLAEFD
                     KNPLVKHAFYGLRAAVVGLITFALLKLLSVTLLTDGAINYLNAGLYLVFVALVLIFKK
                     AHPLIWILSGALIGLILGLPS"
     gene            complement(3377..3949)
                     /locus_tag="PLF67_08540"
     CDS             complement(3377..3949)
                     /locus_tag="PLF67_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE90095.1"
                     /translation="MKYVGITTILLALLISGCSSTNAERLTLDAFQHIDEGGIEEALP
                     LFEDACRKQPENPEYLYNLLYAKLRNMEFESVIEKSGEAFMRHPLYLEFLYLKARAQR
                     ELNRTDAAIATYREIARLNPGDVKALALLLSEAATHGLQEEAVYLAQVILDADPSNMT
                     ALKTLAEADPSSWFALVYAYLMKEDPGSVQ"
     gene            complement(3946..5010)
                     /locus_tag="PLF67_08545"
     CDS             complement(3946..5010)
                     /locus_tag="PLF67_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOE90096.1"
                     /translation="MNKITLHATDLDGYLLDADKAKIDEANRLYKEYLEHCSKLDRAM
                     NHDETVAERNNLVVKAREIGRFLKEVCSNEPNIHVYSFETPQEQHGAASRLISKLRNP
                     VTGNEEFLYYVQRAYELLFSHVWADAVLPTKRSVITRTPVTVPVQNYAVHRIPDVDSI
                     INNSVMCVMLRGALLPSMIVSKEIQEYSSIGYVTPFALFKIKRDDSKKESNMDYILDL
                     DKSYFDLQQLDGKDLIFADPMNATGGSLVTIAKYLKEHNVRPKSVKFINIISALKGSL
                     RVVRAIEGAQVYTLWMDPVLNDAAYILPGLGDAGDRLNGKDSESNPRNIIQLLADYGA
                     GITNLYRSQVREIEQTVLER"
     gene            complement(5066..5239)
                     /locus_tag="PLF67_08550"
     CDS             complement(5066..5239)
                     /locus_tag="PLF67_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90097.1"
                     /translation="MYRVLAEYKKVAPDVTIRFQPTNSGTGPDPTLLTLAAIPVLGLY
                     PGMSSQFIKGVKG"
     gene            5492..6133
                     /locus_tag="PLF67_08555"
     CDS             5492..6133
                     /locus_tag="PLF67_08555"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOE90098.1"
                     /translation="MNLIFLGPPGAGKGTVADLAKDALGIPHISSGQLFREQIKIGSP
                     LGKRVEATLKAGNLVDDETTIEFVRERLSRDDVARGYILDGFPRTIAQAEAMCVFSKP
                     EAVINFSLTEEEILRRLEGRRVAPSTGRTYHILYNPPKVEGLDDETGETLIQRPDDKK
                     EAILNRLEVYTRETRPLINHYREKNLLVPLDASPSPQEVLNQLLHLLKEKGIH"
     gene            6144..>6369
                     /locus_tag="PLF67_08560"
     CDS             6144..>6369
                     /locus_tag="PLF67_08560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOE90099.1"
                     /translation="MATYHNEPSRTFSEYLLIPGYSDRNCVPANVSLATPLVRYRKGM
                     EECPLSLQIPLTSAIMQSVSGEKMAIALARE"
BASE COUNT         1472 a         1710 c         1770 g         1417 t
ORIGIN      
        1 agaaagaaac gcataaggcg gtggcaaagg aggagatcag aagcagacga tagcggacgc
       61 tctccaccgc cagtccgctt gagcgcgccg aaccttcatc aagcagaagc agatcaaggt
      121 ttcgatggtt tccaatgagg aagaggagaa gaagaaccga gacgaggaaa gcgatgacga
      181 cctgattcca ggtggcggtg gaaaaggttc ccagcgacca ggcgagaatc cgctgatatt
      241 gctcccggtt cagaaagagg agcagcgtca tcgctgctga aagaatatag ttcgcagcca
      301 ctccgctgag cagaaggacg gtgttgcttg accgttttcg gcttgccagg gcaaggatga
      361 agagggtcgt ccccgtcgcc cccagcaggg cggagagggg aagaccgaac cgggtagcaa
      421 cgccgaaata gattgcaaga gcgaccccga aggaggctcc gctgctgacc cccagaatga
      481 aggggtcggc catcggattg cgaagaaccg cctggtagga gacgcctccg agggcgagga
      541 tcgtcccggc cgcgaaggag gcgatgatcc tcaccaaacg caggttgagg atgatatacc
      601 gttgggctga agaggtgttc cggccggtga acactccgat cgtctcggac aagccaatcc
      661 tggaggagcc gatcgtaagc gagacgagcg ccagaatcag cgttgccatc aggatgatcc
      721 atccgaggtg ccgcctcatc ctatcgttcc ccgtcgatca ggtcgaagag gatctgaagc
      781 gcctgcgcgc ttcggggacc ttggcgcaga acgacgtccg catcgaagcc tttgaccgtc
      841 gctttcaggt cgctgtacgg cttggtcgtc gtgaactcgc ggatcgtcgc ctcttcgctt
      901 tctccccacc gaggcgaaag aaggatcagc tggggctcct tttcgaccag cagctccttg
      961 gagtaagtcc agtaccggcc gtccgcggcg acgttcgtcc ctccggcttt ttcgatcatc
     1021 tcatggagga aggtatcgcc ggtcgccgcc gaatcgaagg agccgaaatc cagggccatg
     1081 tagaccgtga ccttcgatag cggggcggcc ttctcttcga tcgccttgac ttgggccttc
     1141 atttctccaa tgagctgctc cgcttggctt tgcctgtgaa tgacatcgcc aacggcccgg
     1201 atcagatcat aggttccttc gaacgtctcc tgagcactga tcgaaagcac gttgatattc
     1261 gcctgctcga tggaaaagag cagttcatcg ggcacgaatg ccgaggagat cacatgggtc
     1321 ggctcaagag caagaatcat ctcaagggag ggattgtaga gcgtcccgac gctcggaagc
     1381 gagtcggctt ccgccggata gttgcagtag tcgctccggc cgaccagaag gtctccgccc
     1441 ccgagggcat agatcgtttc cgtcacgttt ggtgaaagag agacgacccg ctgttcgacg
     1501 ggctgttcca tcatcccctg agcggaaagc ggtgcgagca tcagcacgat catgctgatc
     1561 acgatgaaaa gcggtgttag aaaatgacgt ttcatgacgc ccctttatgg gcatgagaca
     1621 tgccttgacc tatcatccgt aggtcttcgg atattatccg gatcaggtct cctggctcag
     1681 gcgttatcag aatcgacgac ttcccacttc cgcagtgtcc ttatgccgat tcccaaacct
     1741 ctacagtggc gggaccgcag gggcgtttga cccctttccc ttgttccgga caccccatcg
     1801 tacaacagtt tcccttcttc gggcaagcgt tgaccaactc aataccctgt actactatcc
     1861 gaatgatgag ttaccacgaa atgacaatct ataccgacgg cggatgcatc ggaaatcccg
     1921 gtgtcggagc ctgggcgtat gttctcgaag cggacggcgt cttcgtgaaa gagggaagcg
     1981 gctatgatcc cgaaacgaca aacaacaaga tggaacttac agcggtgatc gaagcgttga
     2041 aagcggcaag cgcgttgggg gccaggaaga tcactctgta taccgacagc caatacgtta
     2101 aaaacggaat cacatcctgg atcaacaagt ggaaaatcaa cggctggcgg accgcaggca
     2161 gggatccggt gaaaaacaag gagtactggg tcgcccttga tgagctgagg aacctcgccg
     2221 atgtcacttt ttcctgggtg aaaggccatg ccggcatccc gggcaacgaa cgctgtgatg
     2281 cgttggtcgc tcaaacaatc gaggcgcatc aatgaaaaaa acttccttat gggatctcta
     2341 tttcacgttt ttcaagatcg gcggcttgac gttcggcggt gggtatgcaa tgctcccgct
     2401 cctccaggag gaggtcgtga acaaacgccg atgggcgagc gaggaagagt tgctggatat
     2461 gtacgcaata gggcaagtaa cccccggcat catcgcggtg aataccgcaa cgatggtggg
     2521 ctaccgcaag cggggaatcc tcggcggcat catcgccacg ctgggtgaaa tctcccccag
     2581 catcctgatc atcaccacgt tggcgacaat ccttcttcgc tacaaagatg ccgtttacct
     2641 tcagcaggcg ttcgtcggaa tccgaatcgc cgtgtgcgcc acggtcaccg tatcaatcat
     2701 caagctgatg aaaaagagtt tggtggatct gccgacagtt ctgttctacc ttcttgttgt
     2761 ggcttcgacg ttgatgtttt cgctttctcc gatcattgtc gtcatcgtcg ccattctttt
     2821 cggtatcgta gttcagaaca taaagcacaa gaaggaacgg acatgatcta tctcacgctc
     2881 ttctacgagt tcttcaagat cggtctgttt tcaatcggag gagggttggc caccctaccg
     2941 ttcctctaca agcttgcaga aaaaagaccc gattggctta ccatccaaac gatcgccgac
     3001 atgatagcgg tcagcgaatc gacccccgga ccgatcggaa tcaatatggc gacctacgcc
     3061 ggctatcaag tggcgggaat cttcggcgga atcatcgcca cactcggcga agtcgccccc
     3121 tcgatcatca tcatcgtgat catcgccaag tttttagcgg agtttgataa gaacccgctt
     3181 gtaaagcatg cattctacgg cctgagggcc gccgtggtcg gactgatcac cttcgctctt
     3241 ttgaagctgt tgtccgtcac cctgctgaca gacggtgcga tcaactacct caatgccggc
     3301 ttgtacctcg tctttgttgc gctcgtgctc atcttcaaga aagcccatcc gctgatctgg
     3361 atactatcgg gagcgcttat tggactgatc ctgggtcttc cttcataagg tacgcataga
     3421 caagcgcaaa ccaagaggaa ggatccgcct ccgccaatgt tttcagtgca gtcatgtttg
     3481 agggatccgc atccaaaatc acttgtgcaa ggtacacagc ctcttcctgc aagccgtgcg
     3541 ttgccgcctc cgacagaaga agggcaaggg ctttcacatc tcccggattc aacctggcga
     3601 tctcccgata cgtcgcgatc gctgcatccg ttcggttcag ttcccgctgc gcccgggctt
     3661 tcaggtaaag aaattcaagg tataggggat ggcgcatgaa cgcctctcca cttttttcga
     3721 tcaccgattc aaactccatg ttccggagct tcgcataaag aagattgtac aggtactcgg
     3781 ggttttcggg ttgcttccgg caggcatctt caaagagggg caaggcttcc tcgattccgc
     3841 cttcatcgat gtgctggaac gcgtcaaggg tgaggcgctc ggcattcgtg gaggaacatc
     3901 cgcttataag gagagcaaga aggatggtcg tgatgccgac gtacttcatc gctccaagac
     3961 tgtctgttca atctcccgta cttgcgaacg gtagagattg gtgattccgg caccgtagtc
     4021 ggccaatagc tggatgatgt tgcggggatt ggattcgctg tcctttccgt tgagccggtc
     4081 accggcatcg ccaagtcccg ggaggatata tgcagcatcg ttgagcacgg gatccatcca
     4141 aagggtatac acttgagctc cttcgatcgc ccggacgacc ctcagcgatc ctttgagggc
     4201 gctgatgata ttgatgaatt tcacgctttt gggtctgacg ttgtgttctt tcaggtattt
     4261 ggcgatcgta acgagcgaac cgccggttgc gttcatcgga tcggcaaaga tcaaatcctt
     4321 gccgtccaac tgctgaagat cgaagtagga tttatccaga tcgaggatat aatccatgtt
     4381 cgactccttt ttggaatcat cccgcttgat cttgaacaag gcgaagggtg tgacgtatcc
     4441 gatggaagag tactcctgaa tctccttgct cacgatcatc gaaggaagca gcgccccgcg
     4501 gagcatcacg cacatcaccg agttgttgat gatcgaatca acgtcgggga ttcggtgcac
     4561 agcatagttc tggaccggga cggtgacggg ggtgcgggtg atgaccgagc gcttggtggg
     4621 aagaaccgcg tccgcccaaa catgtgaaaa gaggagctca tacgcccgtt gaacatagta
     4681 gaggaactcc tcgttgccgg tgaccgggtt tctcagcttg ctgatcaggc ggctggccgc
     4741 tccgtgctgc tcctgcggcg tttcaaagga atagacgtgg atattcggtt cgttggaaca
     4801 cacttctttc aaaaagcgcc cgatctcccg agcttttacc acaagattgt ttcgctcggc
     4861 gacggtctcg tcgtggttca tggcgcgatc aagtttcgaa caatgctcca aatactcttt
     4921 gtacagcctg ttcgcttcat cgatcttcgc cttgtcggca tccagcagat acccgtcaag
     4981 gtccgttgca tggagggtga tcttgttcat cggctcgttc tcctacgccg attctagcgc
     5041 aagatcgccc cttgcacaac cgtgatcacc ccttcacccc tttgatgaac tgagaggaca
     5101 ttcccgggta cagaccgagc acgggaatcg ccgcaagggt gagcagcgta ggatcgggac
     5161 cggtacccga gtttgtcggt tgaaagcgaa ttgttacatc cggtgcgacc ttcttgtact
     5221 ccgcgagcac ccgatacatc cgctcgccag gagcccatcg caagagtgac cgctgatgga
     5281 gcttttgcgc cctgtgctgt aagtggcagt aaaactagaa gtagaatcaa tacagaaaaa
     5341 agttcatacg gaacttcttt ttgtgttttc agcgactgta tgccatcttt cggggcgtat
     5401 ttgccaaaaa tctattaagt aattgtaaaa tcctagtgcc taaagattct gaatgtggta
     5461 cggtggttag ggaattcaag tgaggtagat catgaaccta atttttcttg gtcctccggg
     5521 agcaggcaaa gggacggttg ccgacctggc gaaagacgcg ctggggatcc cccatatttc
     5581 aagcggacag ctgttcagag agcagatcaa gatcggatct cccttgggca agcgggtgga
     5641 agcgacgctc aaggccggaa atttggtgga tgatgaaacg acgatcgagt tcgtccggga
     5701 gcgcctttcc cgcgatgacg tggctcgagg atacatcctg gatggatttc cccggacgat
     5761 cgcccaggcc gaagcgatgt gcgtcttctc caagccggaa gcggtgatca acttttccct
     5821 caccgaagaa gagattcttc gccgcctgga gggaaggcgg gtcgctcctt caacgggaag
     5881 aacctatcac atcctctaca atccgccgaa ggttgaaggg cttgatgatg aaaccgggga
     5941 aacgttgatt caacggccgg atgacaagaa ggaagcgatc ttgaatcgct tggaagtcta
     6001 cacgagagaa acccggccct tgatcaatca ctaccgcgaa aagaatctct tggtgcccct
     6061 cgacgcttca ccgagtcccc aagaggttct caaccaactg ttgcacctgt tgaaggaaaa
     6121 gggcatccac taaaggaggt tccatggcca cctatcacaa tgagccctcg cgaacgttca
     6181 gcgagtatct cctgattccg ggatattccg atcgaaactg cgtcccggcc aacgtttcac
     6241 tggcaacacc gcttgttcgt tacaggaagg gaatggaaga gtgtccgctt tcccttcaga
     6301 taccccttac cagtgcgatc atgcaatcgg taagcggaga gaagatggcg atcgccctcg
     6361 cccgggaag
//
LOCUS       DAOSLI010000080         6162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_11534_length_6162_cov_11.516293, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000080 DAOSLI010000000
VERSION     DAOSLI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11534_length_6162_cov_11.516293"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLF67_08565"
     CDS             <1..374
                     /locus_tag="PLF67_08565"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOE90100.1"
                     /translation="AKFFRTPIEEVLPVDIYTFDDRVETPEGTEITIVDPDHPILDGI
                     KGPWPHLLGYQEAPLKGDAHLIAQSQYGHPLIAVRKYGKGTSMVWMSDIGPHWCPLEF
                     TRWEGYATFWRNAIHYLANDR"
     gene            622..1881
                     /locus_tag="PLF67_08570"
     CDS             622..1881
                     /locus_tag="PLF67_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOE90101.1"
                     /translation="MKKVGTILLLLIVAGTFLFAGGKAETVKSGPLSVMIWDSNQEPG
                     IRKIIDDFTAKTGIEAEIQVVDWANYWTLLAAGAQGGSLPDVFWMHSNESQRYMSNEM
                     LLDITDRVAKSSLIDLANYPADIWSLYTYENRYYAVPKDVDTIALWYNKALFDKAGLS
                     YPDASWTWDDLYVAAKKLTAPDRSVYGFANQSNNNQAGWYNLVYGNNGYVLSEDKTHS
                     GLDKPEAIEAMKWMERMILEDLMPSQSVMSENAEDVLMQSGKVVMAMQGSWMVPSFRD
                     NEYSYENCDVAELPYNARTGRRPSIYNGLGWAIAKNSTRADDAWKLVEYFGSKEAQLK
                     QAQLGITMSAYVNTSEDWKNSVPKFNLQAYLNMQDDMVIRPFSKNTVKWEDATHDIML
                     KVWSKEITMEQGMREAARITNEILADE"
     gene            1938..2834
                     /locus_tag="PLF67_08575"
     CDS             1938..2834
                     /locus_tag="PLF67_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE90102.1"
                     /translation="MRNNSLRSKTAGQTFFWGWVLILPTVVGLMVLNIVPLITTIYQS
                     LHKTGDFGRGYIFIGLKNYEKLFSDKLVRQSIINTFKYTIIQVPFSVFIALVLAVMLN
                     RENMRGRSAYRTIFFLPMVVAPAAIAMIWRWLLNTEFGLINNLFNIRIGWLTDPRFGI
                     YSVAIIGIWSDIGYNMILFLAGLQEVPRDYYEAAELDGANVIQKFASVTVPLISPVLF
                     FVIVTRMIAAMQVFDTIFMMMERANPALAKTQSLVYRFYQASFIVKEIGYGSAIVIVL
                     LLFILAITAVQLIAQKRWVHYS"
     gene            2845..3678
                     /locus_tag="PLF67_08580"
     CDS             2845..3678
                     /locus_tag="PLF67_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE90103.1"
                     /translation="MSTKKMNLTTVFMHAVLIALACTMVLPFVWMTLTSFKTNAESIQ
                     LDPYLFFPTKLRFTAFETVLKHNNFLVLYKNTILLVGLRILCAIVTASMAGYAFGRLK
                     FPGSTIAFSIVLIQMMIPSQIFIIPQYMMIIRMKATNTIFALVFPGLVSAFGTFLMRQ
                     TYAMLPKDLEDAAKIDGLNIPKTFLYIMAPLTKSSLIALSVFTAVFAYKELMWPMIVN
                     TSKDSLVLASALAKIKGQYLANYPEVMAASLIACVPMIIFYFFFQRQFIEGIATSGGK
                     L"
     gene            3720..5039
                     /locus_tag="PLF67_08585"
     CDS             3720..5039
                     /locus_tag="PLF67_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOE90104.1"
                     /translation="MKITFLGAGSTVFARNVLGDCMHVPSLRSAEMALYDISAKRLEE
                     SRGILEAINQTINEGRAKIRVYHGMERLEDALRDAAFVVNAIQVGGYDPATLTDFEIP
                     RKYGLRQTIGDTIGIGGIMRGLRTIPVMERFASVMERACPGALLLNYTNPMSILTGYM
                     LRYTDVACVGLCHSVQVCSEMLLKDLGMEDALEGRVETIAGINHMAWLLSLRDKEGND
                     LYPEIRKRALAKNAEEKHGDMVRYEYIRRLGYYCTESSEHNAEYNPFFIKRSYPELID
                     RFNIPLDEYLRRCVDQIEEWGEEFKRISSQGGVDHSRSHEYASYIMNAVATNTPFKIH
                     GNVLNDHLIDNLDVNACVEVPCLVDGNGINPCRVGSLPVHLAAMNMTNVNVHLTTIEA
                     ARTKRVEDIYHAAMLDPHTAAELSIDDIVSMCDEMIEAHGPLMEVYT"
     gene            5117..5983
                     /locus_tag="PLF67_08590"
     CDS             5117..5983
                     /locus_tag="PLF67_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE90105.1"
                     /translation="MPESELLIKAARFVGLDGVRKSAIDLSLAYCGFQQCPPLHNNGP
                     EIRDTSFIHIVLSGKGVLKMDGKLYKIKENEAFLIPAGVEAQYTADRHDPWHYMWVGF
                     SGLMSEECMEMAGLNRKHPVRTVENSDLMKKDIEGLIETYHLTFPNELVRCGYFFHFI
                     AHLIEDYQAQGLIKEQSGIRVARHARLAYHHIMEHFSEPVKIEELANQIGVSRNYLYT
                     CFKRVYGISPKQFLNQIRMEHASSLLSSQHLYVAQIANQVGFEDELAFSSAFKGFYGI
                     SPSEYRKNIRGR"
BASE COUNT         1507 a         1506 c         1647 g         1502 t
ORIGIN      
        1 gcgcgaagtt cttccggacc ccgatcgaag aggtgcttcc ggtggacatc tacacctttg
       61 acgaccgggt ggagacgcct gaaggaaccg agatcacgat cgtcgacccc gaccatccga
      121 tcctcgacgg catcaagggc ccctggccac acctcctggg gtatcaggaa gcgccgttga
      181 agggcgatgc gcacctgatc gcccaaagcc agtacggcca ccccctgatc gccgtgcgga
      241 agtatggaaa gggaacatca atggtctgga tgagtgacat cggcccgcac tggtgtccgt
      301 tggagttcac ccggtgggaa ggatatgcga ccttctggag gaacgcgatc cactatctgg
      361 ctaacgatcg ataatacccg caagaatctc gtccaaagtg gaggatagca tgaatacaat
      421 tctcctgttc ttcttccgag cttatgtcgg ctaagtaata acatttttcc atatatctaa
      481 aacattttcc cattcctttg atgcatggat gccttaccat gctaatgaga cttgagccaa
      541 atatttattt ttatccaaag cagttgttga aaaactaagg ggtattacct ctaaaggaca
      601 cgaaaaagga gaacgtttgt catgaaaaaa gtaggcacta ttttactact tctgattgtc
      661 gccggcacgt tcctgtttgc cggaggaaaa gcggaaaccg tcaagagcgg tccgctttcc
      721 gttatgatct gggattccaa ccaagagccg ggaatccgca agatcatcga cgacttcacc
      781 gcaaagacgg gaatcgaggc agagatccaa gtcgtcgact gggccaacta ttggacgctg
      841 cttgccgccg gcgcgcaagg cggttcgttg ccggatgtat tctggatgca ctcgaacgaa
      901 tcgcaacggt atatgtccaa tgagatgctt ctggatatca ccgacagggt agcaaaaagc
      961 agtttgatcg atttggcgaa ctatccggcg gatatttgga gcttgtatac gtacgaaaat
     1021 cgctattacg cggttccaaa agatgtggat acgatcgcgc tgtggtacaa taaggcgctt
     1081 tttgataaag cgggactctc ctatcctgat gcaagttgga catgggacga tctgtacgtg
     1141 gcagccaaga agcttaccgc acccgaccgc tcggtatatg gatttgcgaa ccaatccaac
     1201 aacaatcagg ccggctggta caaccttgta tatgggaaca acggctatgt cctcagcgaa
     1261 gacaaaacgc attccggtct tgataagccc gaagcgatcg aagcgatgaa atggatggag
     1321 cggatgattt tggaggatct catgccttcc cagagtgtga tgagcgagaa cgccgaagat
     1381 gtcttgatgc aatccggcaa ggtcgtaatg gcgatgcagg gatcatggat ggttccttcg
     1441 ttccgggaca atgaatactc gtatgaaaac tgcgacgtgg ccgagttgcc gtacaatgcc
     1501 cgcaccggcc gccggccctc catctacaat ggtcttggct gggcgatcgc caaaaactcc
     1561 acccgtgccg atgatgcatg gaaattggtc gaatatttcg gttccaagga agcgcagctg
     1621 aagcaggccc agcttggaat cacgatgagc gcatatgtga acaccagtga agattggaaa
     1681 aacagtgttc cgaagtttaa tttgcaagcg tacctgaaca tgcaggacga catggtcatc
     1741 cgtcccttct cgaagaacac ggtgaagtgg gaggatgcga cccatgatat catgctcaag
     1801 gtctggtcga aggaaatcac gatggagcag ggcatgcggg aagccgcccg gatcaccaac
     1861 gagattcttg ctgacgaata atcgatgcac tatgattggg cggaggactt tccgcccgat
     1921 cattccatga ggtttgaatg aggaataatt cattacgctc caagaccgcc ggacaaacct
     1981 ttttttgggg atgggtgctg atccttccca cggtcgtcgg attgatggtt ttgaatatcg
     2041 ttccgctcat cacgacgatc tatcagagtc tccataagac cggggatttc ggaagagggt
     2101 atatcttcat cgggttgaag aactacgaaa agcttttttc cgacaaattg gttcgtcaat
     2161 caatcataaa cacattcaaa tacacaatca ttcaagtgcc gttttccgtt tttattgcct
     2221 tggtgctggc ggtcatgctc aaccgggaga atatgcgggg cagatccgcc tatcgaacca
     2281 ttttctttct tcccatggtc gtcgctcccg ccgcgatcgc gatgatctgg cggtggctac
     2341 tcaacaccga attcggattg atcaacaatc tcttcaacat ccggatcggt tggctgacgg
     2401 atccgagatt cgggatatac tccgtggcga tcatcgggat atggtcggat atcgggtaca
     2461 acatgatcct ttttctggca ggtcttcagg aagttcctcg ggattactat gaagccgccg
     2521 aactggatgg ggcgaatgtc attcagaagt ttgcttcggt gacggttccc ctgatctcac
     2581 cggtgctgtt cttcgtcatc gtcacgcgga tgatcgcggc gatgcaggtg tttgatacga
     2641 tcttcatgat gatggagcgg gcgaatccag cccttgcaaa gacccagtcg ctggtatacc
     2701 gcttctatca ggcatcgttc atcgtgaagg aaatcggcta tggttcagcg atcgtcatcg
     2761 tgctcctgct ctttattctt gcgatcaccg ccgtccagct catagcgcag aagcgctggg
     2821 tgcactacag ctaggagggg gagggtgagt acgaagaaga tgaacctgac gacagttttc
     2881 atgcacgcgg tgcttatcgc cctcgcttgt acgatggttc ttccctttgt gtggatgacg
     2941 cttacctctt tcaaaacaaa cgccgagtcg attcaactcg atccatacct ctttttccct
     3001 acaaagcttc ggtttaccgc attcgagacg gtcttgaagc ataataactt ccttgttctc
     3061 tataagaaca cgattctgct ggtcggtttg agaattctct gtgcgatcgt aacggcaagc
     3121 atggccggat acgcctttgg aaggctgaag ttccccggct ccaccatcgc attctccatc
     3181 gtcctgatcc aaatgatgat accatcgcag atcttcatca tcccccagta catgatgatc
     3241 atcaggatga aggcaacgaa tacgattttc gccttggtct ttccgggtct tgtgagcgcg
     3301 ttcggcacgt tcctgatgcg ccagacatat gcgatgcttc ccaaggatct ggaagacgct
     3361 gcgaagatcg acggactgaa tattcccaag acattcttat acatcatggc tcctcttaca
     3421 aaatcgagcc tgatcgccct gagcgtcttt accgcggtat tcgcgtataa ggagctcatg
     3481 tggccgatga tcgtgaacac cagcaaggat tcgctggttc tcgcctcggc gttggcgaag
     3541 atcaaggggc aatatttggc gaattatccg gaagtgatgg cggcttcgct tatcgcctgc
     3601 gtaccgatga ttatattcta tttctttttc caacgccaat tcattgaagg aattgcgacc
     3661 tctggcggaa agctttgagg agattgacca ctgcatttca tctctaagga ggagatttaa
     3721 tgaaaattac ctttttgggt gcggggagca cggtgttcgc acgcaacgtg ctgggagact
     3781 gcatgcatgt tccatcactc cgctcagcgg agatggcgct gtatgatatc agcgccaaac
     3841 gccttgagga atcaagaggc attcttgaag cgatcaatca gacgatcaat gaaggtcgag
     3901 cgaaaatccg ggtgtatcac ggaatggagc ggttggaaga tgcccttcgg gatgctgcct
     3961 tcgtggttaa tgcgatccaa gtcggcggct atgatcccgc aacattgaca gattttgaaa
     4021 tacccagaaa gtatgggctg agacagacga ttggagatac gatcgggatc gggggcatca
     4081 tgcgggggct cagaacgata cccgtcatgg agcggttcgc cagtgtgatg gagcgggcgt
     4141 gccccggagc gttgcttttg aactatacga atccgatgag cattcttacc ggctatatgc
     4201 ttcgatacac cgatgtggcg tgtgtggggc tttgtcacag cgttcaagtc tgttcggaga
     4261 tgttgttgaa agatcttgga atggaggatg cgcttgaagg gcgggtggag accatcgccg
     4321 gcatcaacca catggcttgg cttttgagcc tccgcgataa ggaaggcaat gacctctatc
     4381 ctgagatcag gaaacgggca ttggccaaaa atgcggaaga gaagcatgga gatatggtcc
     4441 gctatgaata catcagacgc ttaggttatt actgcaccga atcaagcgag cacaacgccg
     4501 aatacaaccc cttcttcatc aagcgttcgt atcccgagct gatcgatcga ttcaacatcc
     4561 ctcttgatga gtatttgcga agatgtgtcg atcagattga ggagtggggg gaagagttca
     4621 agcgaatctc ctcccaagga ggggtcgacc attcgcgctc tcatgagtac gcttcctata
     4681 tcatgaatgc ggttgcgacc aatacaccgt tcaagattca cggaaatgtg ctgaacgatc
     4741 atctcatcga caatctcgat gtgaatgcgt gcgtcgaggt tccctgcctg gtcgatggaa
     4801 acggtatcaa cccgtgcagg gttggatcgc ttccggtgca ccttgcggcg atgaacatga
     4861 cgaatgtgaa cgtccatctc accacgatcg aagcggcacg gacgaaacgg gtcgaagata
     4921 tctatcatgc ggcgatgctt gatccgcaca cggcggcgga gctttcgatt gacgacatcg
     4981 tttcgatgtg cgatgaaatg attgaagcgc acgggccttt gatggaagtg tatacatgac
     5041 catggtaaca ttcatgatac atttatacgc cctcttttct ttttaggagg gcgtagcgtt
     5101 tgattcggag gatgcgatgc ctgaaagtga gttgttgatc aaagccgctc gtttcgttgg
     5161 gctggatgga gtgcggaaat ccgccatcga cctgtcactc gcatactgcg gatttcaaca
     5221 atgtccaccg cttcataaca acggtccgga aattcgtgat accagcttca tccacattgt
     5281 tctctccggc aagggggtgt tgaagatgga tggcaagctg tacaagatca aagagaatga
     5341 ggcgttcctg attcccgccg gtgttgaagc tcaatacacc gccgaccggc atgatccctg
     5401 gcattatatg tgggtcgggt tttccggtct gatgagcgag gagtgcatgg aaatggcggg
     5461 tctgaataga aaacatccgg ttcgaaccgt ggaaaacagc gatttgatga aaaaggatat
     5521 cgagggcctg atcgaaacct accacctgac gttccccaac gagctggtac gctgtggata
     5581 ctttttccat ttcatcgccc acctgatcga agactatcag gcccaaggcc tgatcaaaga
     5641 acaaagcggg attcgagtcg cccgtcacgc ccgccttgcg tatcaccata tcatggagca
     5701 tttcagtgag ccggtgaaaa tcgaagagct tgccaatcag atcggggtca gtcggaacta
     5761 cctctatacg tgtttcaagc gagtctacgg aatcagcccc aaacagttcc tcaaccagat
     5821 tcgaatggaa cacgcaagca gtctcctcag ctctcagcat ctttatgtcg ctcagatcgc
     5881 caatcaggtg ggctttgaag acgagcttgc cttttcttcg gcatttaaag ggttttatgg
     5941 gatatctcct tcggagtacc gcaagaatat ccggggaagg tagccggcaa tcgctaatga
     6001 cttgtaacga cctttttaag gaagcgatcc acaagattcg gatccaacac cgtccccttc
     6061 atgcccttca ggcttgcgac cgcgactgat atgggtaccc gttcaggggt ggagttgggg
     6121 ttggtgaggt ttgcccatgc attcgccact gcgatgattc gg
//
LOCUS       DAOSLI010000081         6104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_11659_length_6104_cov_23.510002, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000081 DAOSLI010000000
VERSION     DAOSLI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11659_length_6104_cov_23.510002"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /gene="araA"
                     /locus_tag="PLF67_08595"
     CDS             complement(<1..1382)
                     /gene="araA"
                     /locus_tag="PLF67_08595"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOE90106.1"
                     /translation="MDTFKEYEFWFVVGSQFLYGPETLEQVALHARTMTDGLNKKGLL
                     PTNVIYKSTVKSADEITQVIKEANASDRCAGLIVWMHTFSPSKMWINGLALLQKPYMH
                     LHTQFNRMIPNEEIDMDFMNLNQSAHGDREHGFIAARMHRKRKIVVGYWEDEDVQRSI
                     ASWMRSAVGAAESRNLKLVRFGDNMREVAVTEGDKVEAQIKFGWQVNTFPVSELVEQI
                     NTVSEEAIDKRLEELLELYHLNTDDEDSVRYQVQVELALRSFLEEKGAKAFSNTFEDL
                     SGMKQLPGLATQHLMGTGYGYGGEGDWKVSAMTRIVKAMSEGLEGGTSFMEDYTYHLE
                     AGNELVLGAHMLEVCPSLASKKAKIEVHPLGIGGKENPARLVFEGASGPGILISLIDL
                     GTRFRLIVHDVEVVKPIMSMPNLPVARIMWKPMPDLKTGAHAWILAGGSHHSVLSTAA
                     TAEMIEDWAE"
     gene            complement(1399..2376)
                     /locus_tag="PLF67_08600"
     CDS             complement(1399..2376)
                     /locus_tag="PLF67_08600"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE90107.1"
                     /translation="MTTLRKKAETDEQSLHRWRRIEEVLRQLMPFIGFVGLVLVFSIL
                     TKGRILNPRNISLMLSQVYVLMIASLGVFMIMTVGGLDFSQGSILGLTSIVFAWVSPH
                     GIILGIVAAMLAGALMGAFNGFFHVKFKIASFIVTICTMYLFRGLCEYLTTHGPISAS
                     LKVLSLDETWLKILLTTIVITIGFLVIHFTRLGYDLRAIGAGEIAARFSGSRPELTTF
                     LVYTFAGMITGFASFINVIRVGSITGTAGSQLETQILIALVLGGMPIGGGAKARFSNI
                     IFGSLTYSVLERSLPMVFPVSATQQLVKGIIFLIVVALTIDRKSLRVIK"
     gene            complement(2389..3888)
                     /locus_tag="PLF67_08605"
     CDS             complement(2389..3888)
                     /locus_tag="PLF67_08605"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE90108.1"
                     /translation="MKEVVLRSENVNKHFGITHAVKDVSLEFYRGEVHGLIGENGSGK
                     STYCSMLSGIHSISSGRFILEGKELHVKSLVEANMNGISIVVQEMGTLSGLTVAENIF
                     LGREHKFMHGFIKDTAQMNRETQQLLNSYGLNRISASTVIDNYSFEDRKLIEIVKATY
                     FEPLVVTIDETTTALSQKGREELFKIIEHLKETNKTVIFISHDLSEVLAHTDRISVLR
                     DGALVKTVNSNEVDADELKRLMVGRELDNRYYRTDYDGTVSDEVVLSVSNLSVPGEFE
                     DISFDLHQGEILGIGGLSECGMHELGKAIFGGSYDRTGSVLLPRKGLEITSIPEAIKH
                     GIAYTSKDRDNESIVLAINIADNITLPSLDILAKKRVLKKKELHAFAQSHADRMSVRM
                     TGIKQYVFDLSGGNKQKVVLARWLGRDSDIFVLDSPTRGIDVKVKADIYALMSELKER
                     GKSIIMISEEILELIGMSDRILIMKNGKFNGTFTRSAALTEEDLIAKMV"
     gene            complement(3898..4734)
                     /locus_tag="PLF67_08610"
     CDS             complement(3898..4734)
                     /locus_tag="PLF67_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE90109.1"
                     /translation="MANYLQTSIQYAVGGCGFYFIVVMGLFDMSIGANIVLSSLVGVL
                     LSRQFGYYGLIFGCLLCGTLIGSFNGFLYTRLRIPSMIVTVGLMLVLESVAAYVVKLD
                     PAGFPGKLTDPNILAFNHAPWNYIVAVSAFLLMAFILRFTKLGTYFNAIGSNEFVAKA
                     MGINVEKYKFIGFVLLHFFVGIMALLTVSYGKGMLAVTGMESMGRNFKPLMGTFFGVA
                     FKKYGMPVSAIVIGEFIITLIFNGLVALNVPTTINDFVTGAVLLIIVTLTNRGARGSV
                     VK"
     gene            complement(4950..6074)
                     /locus_tag="PLF67_08615"
     CDS             complement(4950..6074)
                     /locus_tag="PLF67_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOE90110.1"
                     /translation="MKKAMMILFSVILVSSLLFAGGAGEKASTAKPLIGVSIWSSTDT
                     LGSQVKRMLDAAAKALDVDVMYVDQGHISERVTASAETLVAAGVDGFIICNSASAEMT
                     SVINTANRNKVYTAQFFRVIDPVANPNEHALAVASPYYVGSVHEDEVDNGINLVRILC
                     EKGNRKIALEGWEAGDATFLLRWEGYKKGIELWNAENPKDKAVLLDPQYGGTTSDTGR
                     ATAEAIINANPDIDALIVSGGGGDTLVGALAAIESLGLKGKIDVVSTDFLADLDEQLA
                     TGGMAAESGGHFCDPLFAFMMVYNAIKGNYKVPTDGYYEILFPYLYVASSEDYNAYAK
                     YFVDELPYNAAELREMANMSFDQLKAAAERLSIEDVISRR"
BASE COUNT         1490 a         1659 c         1483 g         1472 t
ORIGIN      
        1 atctccgccc aatcctcgat catctccgct gtcgccgcgg tcgagagcac gctgtggtgg
       61 cttccgcctg caaggatcca tgcatgggcg ccggtcttca ggtccggcat cggcttccac
      121 atgatgcggg ccaccggcag gttgggcatc gacatgatcg gtttcaccac ctcgacatcg
      181 tgaacgatca gacggaacct tgtaccgaga tcgatcagag agatgaggat tcccggtccg
      241 ctcgcccctt caaaaaccaa gcgggcggga ttctccttgc ccccgatacc cagcgggtgg
      301 acttcgatct tcgccttctt ggaggcaagc gagggacata cctcaagcat gtgagcaccg
      361 agaaccagct cgttgcccgc ttccaagtgg taggtataat cctccatgaa cgaagttcca
      421 ccttccaagc cttcactcat cgctttcacg atacgggtca tcgccgagac tttccaatcc
      481 ccttcaccgc cgtagccgta gccggtaccc atcaggtgtt gagtcgccaa accgggaagc
      541 tgcttcatcc cgctgaggtc ctcaaaggtg ttgctgaacg ccttcgcgcc cttctcttcc
      601 aggaatgaac gaagggcgag ctcgacctgc acttgatagc gaaccgagtc ttcgtcatcg
      661 gtgttgaggt gatacagctc cagaagctcc tcaaggcgct tgtcgatcgc ctcttcggag
      721 acggtattga tctgctccac aagctcactg acgggaaacg tattcacctg ccagccaaac
      781 ttgatctgcg cttcgacttt gtcaccttcg gttacggcca cttcgcgcat attgtctccg
      841 aagcggacca gcttcagatt ccggctctcc gccgctccga ccgccgagcg catccaggaa
      901 gcgatcgagc gctgaacatc ttcatcctcc caatacccga cgacgatttt tcgcttccgg
      961 tgcattcggg ctgcaataaa cccgtgctcg cggtctccat gggcgctttg gttgaggttc
     1021 atgaaatcca tatcgatctc ttcgttggga atcattcgat tgaactgggt atgaaggtgc
     1081 atatacggtt tttgcaggag cgcaagcccg ttgatccaca tctttgaggg actgaacgtg
     1141 tgcatccaga cgatcaagcc ggcacatcgg tcgcttgcat tcgcctcttt tatcacctga
     1201 gtgatctcgt cggcgctctt gaccgtcgac ttatagatca cgttcgtcgg caacagtcct
     1261 tttttattca aaccgtcagt catcgtccga gcgtgaagag caacctgctc aagggtctcg
     1321 ggaccataga gaaactggga acccaccaca aaccaaaact catactcttt gaatgtgtcc
     1381 atacactcct ccaaccaatt atttaattac cctgagagac tttctatcga tcgtcaaggc
     1441 gacaacgatc aggaagatga ttcctttcac caactgctgg gtagcggaaa cggggaacac
     1501 catcggcaaa ctcctttcca gaacggagta cgtcagtgat ccgaagatga tatttgaaaa
     1561 gcgggctttt gcacccccgc cgatcggcat gcccccgaga acgagggcga taaggatttg
     1621 cgtttccaat tggcttccgg ccgtaccggt gatcgatcct actctaatga cgttgatgaa
     1681 ggaagcaaaa ccggtgatca ttccggcaaa cgtgtatacc agaaaggtcg tcagctcggg
     1741 gcgggagccg gagaaacggg cggcgatctc ccctgctccg atcgctcgca gatcgtagcc
     1801 gagccgggta aagtgaatca ccaggaaccc gatcgtgatg acgatcgtcg tcaacaggat
     1861 tttcagccac gtctcatcaa gcgaaaggac tttgaggctg gcggaaatcg gaccgtgagt
     1921 cgtcaaatac tcacacaatc ccctgaagag gtacatcgta caaatcgtaa cgatgaagga
     1981 agcaatttta aactttacat ggaagaaccc gttaaaagca cccatcagcg ctccggcgag
     2041 catcgccgct acaatgccaa gaataattcc atgcgggctt acccaggcga agacgatcga
     2101 ggtgagtcca aggatcgatc cttgggagaa atccaaccct cccaccgtca tgatcatgaa
     2161 tactccaagg cttgcaatca tcaaaacata tacctgactg agcatcaggc tgatgtttct
     2221 cgggttcagg attcgtcctt tggtaagaat cgagaacaca agcaccaagc cgacaaagcc
     2281 gatgaacggc atcagttgcc ggagtacctc ctcaattctt cgccatcggt ggagagattg
     2341 ctcgtctgtt tcagctttct tacgtagagt cgtcattatc tatcccccct ataccatctt
     2401 ggcgatcaaa tcttcttcgg tcagtgctgc ggaacgggta aatgttccgt tgaacttccc
     2461 gtttttcatg atgaggatcc gatcactcat tccgatcagc tcaagaatct cctcactgat
     2521 catgatgatc gacttccccc gttccttcag ctcgctcatc agtgcgtaaa tatccgcttt
     2581 taccttgacg tcgatgcctc gagtcgggct gtcaagaaca aagatatcgg aatcacggcc
     2641 aagccagcgg gccaagacca ccttctgctt gtttcccccg gaaaggtcga agacatactg
     2701 tttgatgccg gtcatccgga cactcatccg atcggcatgg ctctgggcaa atgcatgtaa
     2761 ttccttcttt ttcaacactc gcttttttgc aagaatgtcc aaggaaggca aggtgatatt
     2821 atcggcgatg ttgatggcca agacgattga ttcgttatcc cggtctttgg atgtgtaggc
     2881 gattccatgc ttgatcgctt cggggatgga ggtgatctcc aatcctttcc ggggaagcag
     2941 aaccgaaccg gtccggtcat acgacccgcc gaagatcgct ttccccagtt catgcattcc
     3001 acattcactc aacccgccga ttccaaggat ttcaccttga tgcagatcaa atgagatgtc
     3061 ttcaaactcg ccgggaacgg aaaggttgga aacgctgagg acgacttcat cggaaaccgt
     3121 tccatcgtaa tcggttcgat agtatcggtt gtccagctcg cgtccgacca tcagtcgttt
     3181 caattcatca gcatcgactt cattgctgtt gacggttttc accagtgcgc cgtcgcgcaa
     3241 gacgctgatt cgatcggtat gagccagaac ctccgaaagg tcgtgggaaa tgaagatgac
     3301 ggtcttattg gtctctttga ggtgctcgat gatcttgaag agctcttccc ttcccttctg
     3361 gctgagggct gtcgttgttt catcaatcgt tacgaccaac ggttcaaaat aggtggcttt
     3421 gacgatttcg atcagctttc ggtcttcaaa gctgtagttg tcgatcaccg tcgaggcact
     3481 gattcggttg agaccgtagg agttaagcag ctgctgggtc tccctgttca tctgagcggt
     3541 gtctttgatg aacccatgca tgaacttatg ttctcttccc agaaagatat tctccgccac
     3601 ggtgaggccg gataatgttc ccatctcttg aacaacgatc gaaataccgt tcatattcgc
     3661 ttcaacgagg gatttgacgt gcagctcctt tccttcaaga atgaatcgtc cgctggatat
     3721 ggaatgaatc ccgctgagca tcgagcagta ggtggacttg cccgagccgt tttccccgat
     3781 gagcccgtga acctctcccc gatagaactc caaagagaca tctttcaccg catgggtgat
     3841 gccaaaatgc ttattaacgt tttcggagcg taaaactact tctttcattc cagactccta
     3901 tttcacaacg cttccgcggg ctccgcggtt ggtcagcgtc acaatgatca acaatacggc
     3961 accggttaca aagtcattga tcgtggtggg aacgttcaag gcgaccagac cgttgaagat
     4021 cagggtgatg atgaactccc cgatgacgat cgcactgacg ggcattccgt attttttaaa
     4081 ggccactccg aagaaggtcc ccatcaaggg tttaaagttt cgacccatgg attccatacc
     4141 ggtaacggcg agcattcctt tgccgtaact aacggtgagc aaagccatga ttccgacaaa
     4201 gaagtgaagc aggacaaagc cgatgaactt atacttctcg acgttgatgc ccatcgcctt
     4261 ggctacaaac tcattggaac cgatcgcatt gaagtaggtt cccagtttgg taaacctgag
     4321 aatgaaggcc atcaacaaga atgccgacac cgcgacgata tagttccacg gagcatggtt
     4381 gaaagcaaga atattcgggt ctgtcaattt gccggggaac cctgccggat cgagcttcac
     4441 aacataggct gcgacacttt caagaacaag catcaagccg accgttacga tcatcgacgg
     4501 gattctcagg cgagtataga gaaaaccatt gaaagaaccg atcaacgtac cacagagcag
     4561 acatccgaaa atgaggccgt agtagccaaa ctgtcgggaa agaagcactc cgacaagcga
     4621 tgagaggacg atgttcgctc cgatgctcat atcaaagagc cccatcacta caatgaagta
     4681 gaatccgcat cctccgactg cgtattgaat cgatgtttga aggtaattgg ccattaactt
     4741 ggatgtacca aaattatgag gtgaaagaat cttgaagaaa gcccagaaaa agagggccaa
     4801 cccaaagagc aaagcatagc caaaatactt acttcgctta aacctgctga tcgtttcgtt
     4861 cattactttt cctaaagctc aagaaagcaa gccgaaaacc tgttttcggc ttgccttttg
     4921 gacactgggg cgaatcctca gacctcgcat tagcgacggc tgatgacatc ctcaatcgac
     4981 agacgctctg ctgcagcttt cagctgatca aagctcatgt tcgccatctc gcgaagctcg
     5041 gcggcgttgt acggaagctc gtcaacaaag tacttcgcat acgcgttgta gtcttcgctg
     5101 gaagcaacgt agaggtaagg gaagaggatc tcatagtacc cgtcagtcgg gactttatag
     5161 ttccccttga tcgcattata caccatcatg aacgcaaaga gcgggtcaca gaagtgtcca
     5221 ccgctttcgg cggccattcc accggttgcc agctgctcat ccaagtcagc gaggaagtcc
     5281 gtggatacaa cgtcaatctt gcctttcagg ccaagggatt cgattgcggc aagtgcgccg
     5341 acaagggtgt ctccaccacc gccggaaacg atcaacgcat caatgtcggg attggcgttg
     5401 atgatggctt cagccgttgc ccgtccggta tcactggtcg ttccgccata ctgcgggtcg
     5461 agcaagacag ccttgtcttt gggattttcg gcgttccaaa gttcgattcc cttcttgtag
     5521 ccttcccagc ggagcaggaa cgttgcatct ccggcttccc atccctcaag ggcgatcttt
     5581 cggttgccct tctcacagag aatccgcacc agattgattc cgttatcgac ttcgtcttcg
     5641 tggaccgagc cgacatagta cggagaagca actgccaacg cgtgttcatt tgggttcgca
     5701 acaggatcga tgaccctgaa gaattgagca gtatatactt tattcctatt tgccgtattg
     5761 attacggagg tcatctccgc cgatgcggag ttgcagataa tgaacccatc gactccggcg
     5821 gctaccaatg tttcagcgga tgcggtaact cgctccgaga tgtgtccttg gtcaacatac
     5881 atgacatcta catcaagagc ctttgcagcg gcatcaagca ttcgcttgac ctggcttccc
     5941 agagtatcgg tgctgctcca aatggaaaca ccaatcaagg gttttgctgt agatgctttt
     6001 tctccggcac cccctgcaaa caggagcgaa gagaccagga tcactgaaaa aagaatcatc
     6061 attgcttttt tcatctttaa aatcctcctt atgatttcat gtgg
//
LOCUS       DAOSLI010000082         5971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_11987_length_5971_cov_21.252197, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000082 DAOSLI010000000
VERSION     DAOSLI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11987_length_5971_cov_21.252197"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLF67_08620"
     CDS             complement(<1..378)
                     /locus_tag="PLF67_08620"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HOE90111.1"
                     /translation="MGRRKFERPRGERRYKKMFVISFEGTVTEPSYFRMLVPPKSPII
                     LHCLKGSKKSSPGQVLKRMKQYIRYQNLSSSDEAWLVVDKDDWSDDQLSELYAWKSSS
                     SQRGLAVSNPKFELWLLLHFEDPR"
     gene            complement(386..1720)
                     /locus_tag="PLF67_08625"
     CDS             complement(386..1720)
                     /locus_tag="PLF67_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE90112.1"
                     /translation="MLVRFTLENWMSFRDPVEFSMIASREKQHGDRVPQVSKYQTRIL
                     PISTIYGGNASGKTNFMKALHFAETFVVQGTKPDGSIQIEPFVLDSEMAKRPSRFVFE
                     LLIDELIYEFSFMVTRKKVVEEKLILITSTSERVLYHRQNDKITLNSSLKKNKFLQFV
                     SKGTRENQLFLTTSMYQNVELFLPVYTWFRDTLVLLAPDSRFAQFEQYINESDPLYQT
                     MNRMLIHLDTGITHLGGETMPFNHLPFPQNLKETLMDEVKEGTIVRIMDDSNHDRYIV
                     TRENNELIAKKIITYHSGSMGEVKFEMHQESDGSKRIIDLLPAFLELMEIDSKKVYVI
                     DELDRSLHTLLTRRLLSIYLSACSKGSRSQLIFSTHDVLLMDQQLLRRDEMWVTERED
                     DGNTVLIPFSEFKDIRYDKDIMKSYLQGRLGGIPKILLDKTLLDSECNKGET"
     gene            complement(1869..3149)
                     /locus_tag="PLF67_08630"
     CDS             complement(1869..3149)
                     /locus_tag="PLF67_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HOE90113.1"
                     /translation="MFGRPVGTWSVARNGTHTFQYDETWLASGDSFPISLSIPLGKGV
                     IEGSYIHDFFDNLLPDSEAIRNHMRIRFQAKSLAAFDLLHEVGRDAVGSMQILPEGEL
                     PSTGEITGRVIDEHEIAQTLRGLTQLTSVESVPVDEFRISLPGAQEKTALLNYQGQWM
                     VPHGSTPTTHILKVPLGSFGPLGIDMSSSVENEYLCAKLTKALGIAVAECEIEQFEEI
                     PTLVVTRFDRRWSEDGKQVIRIPQEDFCQVTKTSRADKYESDGGPGIQTIMSILQGSQ
                     NASEDRLTFFSAQLLFWLLAAPDGHAKNFSIFLGSGGRYMLAPLYDIISAYPVLGSGR
                     KQIHPRRLKMAMALQGENRYYNWDKILPRHFVEMGRKYGLDVPTIFQDLAGRIPFAVE
                     AVSNELSPDFPQSVSEPIFEGLIRQGRVLERFTG"
     gene            complement(3181..3435)
                     /locus_tag="PLF67_08635"
     CDS             complement(3181..3435)
                     /locus_tag="PLF67_08635"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE90114.1"
                     /translation="MKFVVNLSHQFGSAVKEIRETKGLRQTDVARQVGMLPKTISALE
                     NRPATVKIESLMKLLSALEYEIILTPKPKQDAFGFNELID"
     gene            3690..4610
                     /locus_tag="PLF67_08640"
     CDS             3690..4610
                     /locus_tag="PLF67_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE90115.1"
                     /translation="MWIIIIIAVVLFILWNTTLIGYRDVPKRQLESNEQNAFSENGKP
                     IIYKGGHSRCVLMVHGFPSTPSMYSYSAKRIHEAGYDVYVPLLPGFGTDPKDLENTTF
                     MQWFDYLCRYYEDLRGKYRTMHIIGTSMGGAMTLKLAERYCSSPSLAPSSIVSIAAPV
                     VYNSIKDGIFTDLRQYVARTIALFIPSIGARIVDGNQNGSDGGQDWVGYGGLMVRPGL
                     SFIASLPKIRKDLNHVTCPLFALHDRGDTTIPFKNLSIIQKETRSSNLVSLETDMKGD
                     GHSRHALLSYYSVQESLTDRIIAFMEEHDA"
     gene            4603..5097
                     /locus_tag="PLF67_08645"
     CDS             4603..5097
                     /locus_tag="PLF67_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOE90116.1"
                     /translation="MHKREDYISWDEYFMGIAQLSAMRSKDPNTQVGACIVSSEHKIV
                     GVGYNGLPIGVDDDLIPWQREGQWLETKYPYVCHAELNAILNAIRSDLKGCRIYVGLF
                     PCNECAKAIIQAGIKEVIYLSDKYAETDNTKASKWMFDQSKVTYRQLKAAHTSLHIDL
                     SCDC"
     gene            5085..>5971
                     /locus_tag="PLF67_08650"
     CDS             5085..>5971
                     /locus_tag="PLF67_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOE90117.1"
                     /translation="MRLLTVCLNPTFQITMVFPSLLLGEVNRAKEHYLDSSGKGMNTA
                     RVLAQLGHDAFLLSHLGRERKHEFIRMAEKDRVTVLWADSKSEIRTCITVLGNGQTTE
                     LIQEPEPVGSGTEGPIRELFTQALETVDGVIITGTRAGGYAHDLYPAFVKEAKQKGLF
                     VLLDLKGDDLRNSLIHRPDIIKPNMCEFVQTFFNRTIREREMDPHLESEVRGLMKQIH
                     DEYGTITYLSRGKEESWIQGETFHTVTPHPIEAVNTIGCGDTLSAVLAVELMEHGDLL
                     RAAETATCWAGVNARQIRPG"
BASE COUNT         1523 a         1603 c         1319 g         1526 t
ORIGIN      
        1 tctgggatct tcaaaatgaa gcaacaacca aagttcaaat tttggattgc tgacagctaa
       61 acctcgttgg ctggatgagc tcttccatgc atataattca gacagctgat catcagacca
      121 atcatcttta tccacgacaa gccatgcttc atcggaagaa ctcagattct ggtaacgtat
      181 atattgcttc atccgtttga gaacttggcc cggtgaactc tttttagatc cttttagaca
      241 atgtaaaatg ataggcgatt ttggaggaac cagcatacgg aaataacttg gctcggttac
      301 ggttccctca aaggatatta caaacatctt cttgtatctg cgctcccctc gaggtcgttc
      361 aaattttctt cttcccatcg tattcctacg tttctccttt attgcactct gaatctaata
      421 atgttttatc gaggaggatt tttggaatac cgcccaaccg tccttgcaga taactcttca
      481 tgatatcttt gtcataccga atatctttaa attcactgaa tggtatcaat accgtgttcc
      541 catcatcttc ccgctcggtt acccacatct catcccgacg aaggagttgt tgatccatca
      601 acaaaacatc atgggttgaa aagatcagtt gagatctact gccttttgaa caagcagaaa
      661 gatatatact taacaatctt cttgtcaaaa gagtatgcaa actacgatcc agttcatcaa
      721 ttacatacac cttttttgaa tcaatctcca tgagctcaag gaatgccggc agcaaatcga
      781 tgattcgctt tgatccatcg gattcttggt gcatttcaaa tttcacttct cccatacttc
      841 cggaatggta cgtaatgatt tttttcgcga ttaattcatt gttttcccgt gttacgatat
      901 acctgtcatg attcgagtca tccataatcc ttacaatagt tccttctttc acctcgtcca
      961 ttaacgtttc tttgagattt tggggaaagg ggagatgatt aaacggcatt gtttccccac
     1021 ccagatgagt aataccggta tcaaggtgta taagcatccg gttcatcgtt tgatataaag
     1081 gatcgctctc attaatgtac tgttcaaatt gtgcaaaccg agaatcagga gcaagcaaca
     1141 cgagggtgtc tctaaaccac gtatacaccg gtaagaaaag ctccacattc tgatacatgg
     1201 aggtggttag aaaaagttgg ttttctctcg ttcctttgga aacaaattgt aagaacttat
     1261 tttttttcaa tgatgagttc aaggtaatct tatcgttttg ccgatgatac aacactcgct
     1321 cgcttgtgct tgtgataagg atgagtttct cctcgactac cttttttcgt gtaaccatga
     1381 agctaaactc atagattagt tcatctatca acaactcaaa cacaaacctt gaaggacgct
     1441 ttgccatttc ggaatccagt acaaatggct ctatttgaat cgatccatcg ggcttggttc
     1501 cttgaacgac aaatgtttcg gcaaaatgca gcgccttcat aaaattcgtt tttccggaag
     1561 catttcctcc atatatcgta gaaattggta agattcttgt ttgatattta gatacttgag
     1621 gaaccctatc gccatgctgt ttttctcgac tggcgatcat tgaaaactct accggatccc
     1681 gaaaagacat ccagttttca agtgtaaagc gtacaagcat agatgcatcc tcctcatatg
     1741 cgctcagtga gctcttcact ataaggataa tccatgccat caataatatc aaccacaaag
     1801 agattatatc tcgttatcca taagaatatt tacaatattt cggtatttca tctatgtaag
     1861 agccgttatc agcctgtaaa cctctccaac acccttccct gtcttatcaa cccctcgaag
     1921 atcggctcgc ttacggattg ggggaaatcg gggctgagct cattgcttac cgcctcaact
     1981 gcaaaaggaa tccttcctgc cagatcttgg aagatggttg gcacatccaa accatatttc
     2041 ctgcccatct ccacgaagtg ccttggcagg attttatccc agttgtaata ccggttttcc
     2101 ccctgaaggg ccatcgccat cttcagcctg cgcggatgga tctgttttct tccgcttcct
     2161 aaaaccggat atgcgctgat gatgtcatac aaaggagcaa gcatatacct ccctccggat
     2221 cccaaaaaga tcgagaagtt tttggcatgt ccgtccggag ccgccaaaag ccagaacaga
     2281 agctgagccg agaagaacgt aagccggtct tcgctcgcat tctgcgaccc ttgaaggata
     2341 ctcatgatcg tttgaattcc cggcccaccg tcgctttcat atttatcggc acgtgaagtt
     2401 ttagttactt ggcaaaagtc ttcctgaggg attcgaatga cctgtttccc atcctcactc
     2461 catctgcgat cgaaccgggt gacgacgagc gtcggaatct cttcaaactg ttcaatctca
     2521 cactcggcaa cggcaatccc caatgccttt gtgagctttg cacagagata ttcattctcg
     2581 acggaggaag acatatcgat tcccaacggc ccaaacgatc caagaggaac tttcaggatg
     2641 tgagtcgttg gagtggaacc gtgaggaacc atccattgtc cctgatagtt caataaagcg
     2701 gttttttcct gagcgcctgg taaggaaatc cgaaactcgt ccaccggaac actctcgacc
     2761 gaagtcaatt gtgtcaatcc tctgagcgtt tgggcgatct catgctcatc aatgaccctg
     2821 cccgtaatct cgccggttga gggaagctca ccttccggca ggatctgcat cgaccccacc
     2881 gcatctcttc cgacctcatg caacagatcg aacgcagcca atgatttcgc ttgaaacctg
     2941 attcgcatgt ggttacgaat cgcttctgaa tcgggcagga gattatcaaa aaaatcgtgt
     3001 atatatgatc cttcgatgac tcccttgcca agcggtatcg atagagaaat gggaaacgaa
     3061 tctccggaag caagccatgt ttcatcgtat tgaaatgtgt gagttccatt ccttgcaaca
     3121 ctccatgtac cgaccggcct tccgaacatc cagatgaaca acctcttcat accttattcc
     3181 tcaatcaatc aattcgttga aaccaaatgc gtcctgcttc ggtttcggag tcagaatgat
     3241 ttcatactcc aaggcggaca gaagcttcat gagactttca atctttactg ttgcagggcg
     3301 attttccagc gctgaaatag tttttggaag cattcctacc tgccgggcga catcagtctg
     3361 gcgaagccct ttcgtttcac gaatctcttt tactgcagaa ccaaattggt gcgatagatt
     3421 gacgacaaac ttcataaagc ctccccatac caaggtcgat atatgggtat gatagtgtgt
     3481 tcttcaatcc cggtcaatat tataccccgc aggggatatt atcaaaatat accctataag
     3541 gtatttttgt attatatccc ctacagggta ttttcactca atgccctact attccttttt
     3601 ttcctatcct tcccttattc atttaaatat gattcttcgt tttacaactc cctcttcttt
     3661 tctccccttt cctgggctat agtgggtgta tgtggattat catcattatt gccgtggttc
     3721 tcttcattct ttggaacacc acgctcatcg ggtaccgtga cgtaccgaaa cggcagctgg
     3781 aaagcaacga gcaaaacgca ttcagtgaaa atggcaaacc catcatctac aaaggcggcc
     3841 attcgcgctg cgtcttgatg gtgcacggct tcccttccac tccgtcgatg tactcctaca
     3901 gcgccaaacg gatccatgaa gccggatacg acgtgtacgt tccgctgctt cccgggttcg
     3961 gcaccgaccc gaaagatctg gagaacacca cgttcatgca gtggttcgac tacctctgtc
     4021 ggtactatga agaccttcga ggcaaataca ggacgatgca catcatcgga acatcgatgg
     4081 gcggggcgat gaccctcaag cttgccgagc ggtattgctc ctccccttca ctggcgccaa
     4141 gcagcatcgt ctcaatcgcc gccccggtgg tgtacaactc gatcaaagac gggattttta
     4201 ccgatttgag gcaatatgtc gcccgcacga tcgccctctt catcccctcg atcggggccc
     4261 ggatcgtcga cgggaaccag aacggctcgg acggaggcca ggactgggtc ggctacggcg
     4321 gcctgatggt ccgcccgggc ctcagcttca tcgcctccct tcccaagatc cgaaaggatc
     4381 tgaaccatgt tacctgtccc ctcttcgcgc tccatgaccg gggggacacc acgatcccct
     4441 tcaagaacct ctcgatcatc cagaaggaaa caaggagcag caatctcgtc tctctggaga
     4501 ccgacatgaa gggtgacggc cattcccggc atgctctctt atcgtattac tcggttcagg
     4561 aatcgctgac cgacaggatc atcgcattca tggaggagca cgatgcataa gcgagaggac
     4621 tatatcagct gggacgagta cttcatggga atcgcccagc tctcggcgat gcgcagcaaa
     4681 gaccccaaca cccaggtggg ggcgtgcatc gtcagtagcg agcacaagat cgtcggcgtc
     4741 gggtacaacg gacttcccat cggcgtggat gacgacctca tcccctggca gcgcgaagga
     4801 cagtggctcg aaacgaagta cccgtatgtc tgccacgccg aactgaacgc gatcctcaac
     4861 gcgatccgca gcgacctgaa aggatgccgc atctatgtcg gcctctttcc ctgcaacgag
     4921 tgcgccaagg cgatcatcca ggcgggcatc aaggaggtaa tctacctctc cgacaaatac
     4981 gccgagaccg acaataccaa agcgtccaaa tggatgttcg accagagcaa agtcacctac
     5041 cgccagctga aagccgccca tacttcattg cacatagacc tttcatgcga ctgttgaccg
     5101 tttgtttgaa ccccaccttc cagatcacga tggtgttccc ctcccttctt ctcggggagg
     5161 tgaaccgagc caaggaacac tacctcgaca gctccggcaa agggatgaac accgcccgag
     5221 tccttgccca attgggtcat gacgctttcc tcttgtccca cctcggaaga gaacgaaagc
     5281 acgaattcat caggatggcc gaaaaagacc gggttaccgt gctgtgggcc gactcgaaga
     5341 gcgagatccg cacctgcatc accgttctcg gcaacgggca gaccaccgag ctgattcagg
     5401 aaccggagcc ggttggaagc ggaaccgagg gaccgatccg agagctgttc acccaagccc
     5461 tcgaaacggt ggacggcgtg atcatcaccg gcacccgggc cggcggctat gcccacgatc
     5521 tctaccccgc attcgtcaaa gaagcgaagc agaaaggact cttcgtcctt ctggacctca
     5581 aaggggatga cctcagaaat tcgctgatcc acagaccgga catcatcaaa cccaacatgt
     5641 gcgaattcgt tcagacattt ttcaaccgta cgattcggga acgggagatg gacccacatc
     5701 tggaatcgga ggtgcgagga ttgatgaaac agattcatga tgaatacgga acgatcacct
     5761 acctttcccg gggaaaggag gagagctgga tccaaggtga aacgttccat accgtcacac
     5821 cccacccgat cgaggcggtc aacacgatcg gctgcgggga caccctctcg gcggttcttg
     5881 ccgtcgagct gatggagcac ggcgatcttc ttcgcgccgc cgaaacggcg acctgttggg
     5941 ccggagtcaa cgcccggcag atccggcccg g
//
LOCUS       DAOSLI010000083         5652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_12868_length_5652_cov_31.508129, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000083 DAOSLI010000000
VERSION     DAOSLI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12868_length_5652_cov_31.508129"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLF67_08655"
     CDS             <1..585
                     /locus_tag="PLF67_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE90118.1"
                     /translation="KTVATFLLAYWLYIDGVDTIIRMAVNYGTTLGFPSESLIIALLI
                     TQFVAFPSALAYSYFGMKVGTKTALKVAIGAYTVIAVLGYFMTKVIHFYLLAVAIGLF
                     QGGIQALSRSYYTRLIPKEQSAQFFGFFNMLGKFAAIIGPFLMGTVTLLTRSSRAGII
                     SLIILFILGYFFLGKVDEEKGKEEVKAFIQSIEI"
     gene            808..2148
                     /gene="mgtE"
                     /locus_tag="PLF67_08660"
     CDS             808..2148
                     /gene="mgtE"
                     /locus_tag="PLF67_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOE90119.1"
                     /translation="MKATFEQLRDYVTSRNWHALQAELSHMQEIDVAAFIESLPAELA
                     LLVFRTLEKDLAAEIFANFSNDQQELLIGAITDRELQHLMKGLMIDDAVDMIGELPAN
                     LVNRILRHTSPKTRSLINQFLLYPEFSAGSIMTAEFVQLKKEMSVKEAIAHIRKVGEN
                     KETIYTCYVTTQNRMLEGIVSVRTLLLSSDEEILETLMDPDYISVTTTDDQEHVADLF
                     RRYDLLSIPVTDHENRLVGIITVDDIIDVIEEENTEDFQKMSALIPSEKPYLKTGVFA
                     LAKNRIVWLMVLLFSGMITGSILARFEDAFMVLPILVSFIPMLTDTGGNAGSQSSTLI
                     IRGMAVNEISMKDLGTVMWKEIRVALIVGIILSFFTVLRIYLMGTAIMIALTVGIALY
                     ATVIMAKTIGGILPMIAKAVHLDPAIMAAPLITTIVDAFSLFLYFTIAQKLLNI"
     gene            2396..3736
                     /gene="mgtE"
                     /locus_tag="PLF67_08665"
     CDS             2396..3736
                     /gene="mgtE"
                     /locus_tag="PLF67_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOE90120.1"
                     /translation="MKATFEQLRDYVTSRNWHALQAELSHMQEIDVAAFIESLPAELA
                     LLVFRTLEKDLAAEIFANFSNDQQELLIGAITDRELQHLMKGLMIDDAVDMIGELPAN
                     LVNRILRHTSPKTRSLINQFLLYPEFSAGSIMTAEFVQLKKEMSVKEAIAHIRKVGEN
                     KETIYTCYVTTQNRMLEGIVSVRTLLLSSDEEILETLMDPDYISVTTTDDQEHVADLF
                     RRYDLLSIPVTDHENRLVGIITVDDIIDVIEEENTEDFQKMSALIPSEKPYLKTGVFA
                     LAKNRIVWLMVLLFSGMITGSILARFEDAFMVLPILVSFIPMLTDTGGNAGSQSSTLI
                     IRGMAVNEISMKDLGTVMWKEIRVALIVGIILSFFTVLRIYLMGTAIMIALTVGIALY
                     ATVIMAKTIGGILPMVAKVTGMNPAIMAAPLITTIVDALSLFLYFSIAQRLLNI"
     gene            3809..5164
                     /locus_tag="PLF67_08670"
     CDS             3809..5164
                     /locus_tag="PLF67_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOE90121.1"
                     /translation="MQPVQENRMGVKAITPLVLSMSFPIMLSMLVQALYNVVDSLFVA
                     HYSHKALTAVSLAFPLQSLVIALSVGTSIGVNSLLSRKLGAKDREGANKTAGNGIILS
                     ILGWAFFAILAILFSKHFITFFSDDPQLIRMGGQYLFVCLFFSLGIFIEINNERVLQS
                     TGDTIRPMLIQFSGAITNIILDPILIFGLLGFPRLGVLGAAIATVIGQHVAALLSIYY
                     VRRNKEVTIAKKDLKLDKRIVRDIYFVGVPAIVMQGIGTILTTSLNKILIAFGTDAVA
                     IYGIYFRLQSFIFMPIFGLNSGMIPIIGYNYGAQRPKRIMKTIRVGITVALIIMAFGT
                     ALFTFFPHVLLALFNASATMLEIGTIALQRLSMCFLMAGVSISLSALFQGMGNGYYSM
                     IISFTRQLVFLLPAAYLLGRFFGLDALWYSFFIAEFSAFFLSLLFFRTIYKNRIKPLY
                     Q"
     gene            complement(5239..5466)
                     /locus_tag="PLF67_08675"
     CDS             complement(5239..5466)
                     /locus_tag="PLF67_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90122.1"
                     /translation="MKRLLTTLLILIVSSLLFGAAIGESVVLYLKGYIAPRAEFFAFE
                     DGAFEYVTNACNFTFEIQDVGSFRMLNVITL"
BASE COUNT         1375 a         1496 c         1337 g         1444 t
ORIGIN      
        1 aagaccgttg caacctttct tcttgcctac tggctctaca ttgacggagt cgacacgatc
       61 attcggatgg cggtaaacta cggaaccacg ttggggtttc ccagcgaatc gctgatcatc
      121 gccctgctga tcacccagtt cgttgcgttc ccttccgcac tggcatactc ctacttcgga
      181 atgaaggtcg gaacaaaaac cgcccttaaa gtagcgatcg gggcatatac cgtgattgcg
      241 gtcctcgggt acttcatgac caaggtgatt cacttctacc tcttggcggt tgcaatcggg
      301 ctgttccaag ggggtatcca agccctctcc cgttcgtatt acacccggct catccctaag
      361 gaacaaagcg ctcaattctt cgggttcttc aacatgctcg gaaagttcgc agcgatcatc
      421 ggtcctttct taatgggtac ggtcaccttg cttacaagaa gcagccgggc gggaatcatc
      481 agcctgatca tcctcttcat ccttgggtat ttcttcttgg gaaaagtcga cgaggagaag
      541 ggtaaggagg aggtcaaagc attcattcag agcattgaga tataaaaact cagctgtgaa
      601 agtggttgaa caatctcatt caactcggta ttctgttggc atatctaccc tattatactg
      661 caaggaggtg cgagtcgtgg atacatgtgg caaacatgca agtatatccg atgaccctct
      721 gagtagaagc ttctgctttg gtgttttcat gcaccgtcat cgttgatgct tgacagttcc
      781 acaccggtct tcgtctccaa tgaaaagatg aaagcaacgt ttgaacagct tcgtgactat
      841 gtgacgtcac gcaattggca tgctctgcaa gcagaactct cccatatgca ggagattgat
      901 gtagccgcct tcattgaaag ccttcccgcc gaattggcgc tgttggtgtt caggactttg
      961 gaaaaggatt tggcggcgga gatctttgcg aacttcagca acgatcagca agaactgctg
     1021 atcggcgcaa tcaccgaccg ggagcttcag cacctgatga aagggctcat gatcgacgat
     1081 gcggtcgata tgatcggcga attgccggcg aatcttgtca accggattct ccgccacacc
     1141 tcgccaaaga cccgaagcct gatcaaccag ttcctcctct accccgagtt ctccgcaggg
     1201 tcgatcatga ccgccgagtt cgtccagctg aagaaggaga tgagcgtcaa ggaagcgatc
     1261 gctcatatcc gaaaggtcgg tgaaaacaag gaaaccattt atacgtgtta cgtcactacc
     1321 cagaatcgga tgcttgaagg aattgtttcc gttcgaacct tgctcctttc ctccgatgaa
     1381 gagatccttg agacattgat ggatcccgac tacatctccg tcacgacaac ggacgatcag
     1441 gaacacgttg cagatctctt ccgccgatat gacctgttga gcattccggt gaccgatcat
     1501 gaaaaccgcc tcgtgggaat catcaccgtc gacgacatca tcgacgtcat tgaagaggag
     1561 aacaccgagg acttccaaaa gatgagcgcc ctgatcccca gcgaaaagcc gtacctgaaa
     1621 accggagtgt ttgccttggc gaagaaccgg attgtctggc tcatggtctt gctcttcagc
     1681 ggaatgatca ccggaagcat ccttgcccgt tttgaagatg cattcatggt gttgccgatt
     1741 cttgtctctt tcattcccat gctcaccgat acgggaggaa acgccggaag ccagagttcg
     1801 accttgatca tccggggaat ggcggtgaac gagatatcga tgaaagactt aggcacggtc
     1861 atgtggaaag agattcgggt agccctgatc gtcggaatca tcctctcgtt cttcaccgtc
     1921 ctacggatct atctcatggg cactgcgatc atgattgccc tcaccgtcgg aatagcgctt
     1981 tatgcaacgg tgatcatggc aaaaacgatc ggcggcatcc tgccgatgat cgccaaagct
     2041 gttcatttgg acccggcgat catggccgcc cccctgatca ccacgatcgt cgatgcgttc
     2101 agtctgttcc tctattttac gatcgcccag aagctcttga acatctaagg tgcctgaatt
     2161 cttgagtgat actcctataa ctgtgaaagt ggttgaacat tcttattcaa cttgatatcc
     2221 tattaataaa taaacactat aatcactaca aggaggtgcg agtcgtggat acatgtggaa
     2281 aacatgcaag tatatctgat gaccctctga gtagaagatt cagttttggt gtgttcatgc
     2341 accgtcatca ttaatactca acagtttctc accggtcttc gtctccaatg aaaagatgaa
     2401 agcaacgttt gaacagcttc gtgactatgt gacgtcacgc aattggcatg ctctgcaagc
     2461 agaactctcc catatgcagg agattgatgt agccgccttc attgaaagcc ttcccgccga
     2521 attggcgctg ttggtgttca ggactttgga aaaggatttg gcggcggaga tctttgcgaa
     2581 cttcagcaac gatcagcaag aactgctgat cggcgcaatc accgaccggg agcttcagca
     2641 cctgatgaaa gggctcatga tcgacgatgc ggtcgatatg atcggcgaat tgccggcgaa
     2701 tcttgtcaac cggattctcc gccacacctc gccaaagacc cgaagcctga tcaaccagtt
     2761 cctcctctac cccgagttct ccgcagggtc gatcatgacc gccgagttcg tccagctgaa
     2821 gaaggagatg agcgtcaagg aagcgatcgc tcatatccga aaggtcggtg aaaacaagga
     2881 aaccatttat acgtgttacg tcactaccca gaatcggatg cttgaaggaa ttgtttccgt
     2941 tcgaaccttg ctcctttcct ccgatgaaga gatccttgag acattgatgg atcccgacta
     3001 catctccgtc acgacaacgg acgatcagga acacgttgca gatctcttcc gccgatatga
     3061 cctgttgagc attccggtga ccgatcatga aaaccgcctc gtgggaatca tcaccgtcga
     3121 cgacatcatc gacgtcattg aagaggagaa caccgaggac ttccaaaaga tgagcgccct
     3181 gatccccagc gaaaagccgt acctgaaaac cggagtgttt gccttggcga agaaccggat
     3241 tgtctggctc atggtcttgc tcttcagcgg aatgatcacc ggaagcatcc ttgcccgttt
     3301 tgaagatgca ttcatggtgt tgccgattct tgtctctttc attcccatgc tcaccgatac
     3361 gggaggaaac gccggaagcc agagttcgac cttgatcatc cggggaatgg cggtgaacga
     3421 gatatcgatg aaagacttag gcacggtcat gtggaaagag attcgggtag ccctgatcgt
     3481 cggaatcatc ctctcgttct tcaccgtcct acggatctat ctcatgggca ctgcgatcat
     3541 gattgccctc accgtcggaa tagcgcttta tgcaacggtg atcatggcaa aaacgatcgg
     3601 cggcatcctg ccgatggtcg ccaaagtgac cggtatgaac ccggcgatca tggctgcccc
     3661 cctgatcacc acgatcgtcg atgcgttgag tctgttcctc tatttttcga tcgcccaaag
     3721 gctcctgaac atctaaggtg ctttaccaaa caggagggct atgatattcc tttagacgct
     3781 gaagcttcag cgtctaaagg atcatcccat gcagccggtg caagaaaata gaatgggagt
     3841 gaaagcgatc actcccttgg tactctcgat gtctttcccc atcatgctca gcatgctggt
     3901 tcaagcgctt tacaacgtgg tcgattcgct ttttgtcgcc cactacagcc ataaagcact
     3961 cactgcagtc tccctcgcat tcccccttca gtcattggtc atcgcattga gcgtcggaac
     4021 atcaatcggt gtgaattccc tgctcagccg aaagctcggg gcgaaggatc gggaaggggc
     4081 gaacaagacg gcggggaacg gaatcattct ctccattctc ggatgggcgt tcttcgcaat
     4141 cttggcaatc ctcttttcca agcatttcat caccttcttc agtgatgatc cccagctcat
     4201 caggatgggt ggtcaatacc tctttgtctg tctcttcttc tcactgggaa tcttcattga
     4261 aatcaacaac gaacgtgtcc tgcaatcgac aggcgacacg atccgtccga tgctcatcca
     4321 attctccggg gcgatcacca acatcatctt ggatccgatt ctcattttcg ggctcttggg
     4381 gtttccccgt ctcggtgttc tgggagcagc gatcgccacc gtgatcggcc aacatgttgc
     4441 ggcgctcctt tccatttact acgtgcggag aaacaaagaa gtaacaatcg ccaaaaagga
     4501 tctgaaactc gataagcgga tcgtgcggga catctacttc gtaggagtcc ctgcgatcgt
     4561 catgcagggg atcggcacca tattgactac cagcctcaac aagatcctca tcgccttcgg
     4621 taccgatgcg gtagcgattt atggaatcta tttccggctc caatccttca tcttcatgcc
     4681 gatcttcggc ttgaacagtg gaatgatacc gatcatcgga tacaactatg gagctcaaag
     4741 accgaagcgg atcatgaaaa caatccgggt gggcatcacc gtcgccttga tcatcatggc
     4801 attcggaacc gccctcttca cctttttccc tcacgtcctg ttagctcttt tcaacgcaag
     4861 tgcgactatg cttgaaatcg gaacgatcgc cttgcagcgt ctcagcatgt gttttctgat
     4921 ggcgggagtt tcgatctccc tttcagcgct ctttcaggga atgggcaacg gatactactc
     4981 aatgatcatc tccttcacgc ggcagctggt cttccttctc cctgcagcct acctgcttgg
     5041 ccgcttcttc ggcctggatg cgctctggta ttccttcttc atcgccgagt tctccgcgtt
     5101 cttcctttcc ctgctcttct tccgcaccat atataagaac cgcatcaaac cgttgtacca
     5161 ataaaagtgg agccaaggct tttcctaccc tggctccttt tgtgcaagct tgtctgtcct
     5221 tacgcgcttg tcttatccct aaagagtgat cacgttgagc attctgaatg atccgacatc
     5281 ctgaatttca aaggtgaagt tgcaggcgtt cgtgacatat tcaaacgctc catcctcaaa
     5341 cgcgaagaat tcggcacgcg gagcgatata gcctttgagg tagagaacca cactctcacc
     5401 tatcgccgca ccgaacagca ggctactgac tatgaggatt aataatgtgg ttaacagcct
     5461 tttcatgctt acctctttat cggtcttaca tgtacatcct aagaggtaaa ttgtaagatg
     5521 tcaatagaaa tattaattaa ttccttatat tatagatatt tataaaatgt catatgttag
     5581 aggtttgcat gttatacata tctccaagtt aggacatatt acatcacaat ttgatagaac
     5641 catcggaaca tc
//
LOCUS       DAOSLI010000084         5646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_12883_length_5646_cov_22.954928, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000084 DAOSLI010000000
VERSION     DAOSLI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12883_length_5646_cov_22.954928"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1332)
                     /locus_tag="PLF67_08680"
     CDS             complement(<1..>1332)
                     /locus_tag="PLF67_08680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HOE90123.1"
                     /translation="TKQDDLILAVTLKSDGSVDKEVIASVAAAKPVEPAFPEDWAFFK
                     RTFANPSLQMVSFTITEKAYAIRSNDGTLFEPIAKDIEEGAKAPKSFLAKLTSLLYHR
                     YREKGGNLTLVSMDNCSANGDRIAEAVKTIGKGWAERGHVEQEFLAYLDENVAYPWSM
                     IDKITPRPDAKIVEILKKDGFKSGGLIVTKNNAHIAFFVNAEEAEYLVIEDTFKNGRP
                     PLDKVGVYFTDRSTVNKVERMKVTTCLNPLHTALAIFGSILGFTLISEEMKDPQLRAL
                     VEGIGYTEGMPVVVDPGIIDPRQFIDEVLNKRFPNPFMPDSPQRIATDTSQKLAIRFG
                     ETVKSYLEHPDLDIKDLKLIPLVFAGWLRYLMGIDDEGRPFTPSSDPRLEEAQEYVKG
                     IKLGDKGPFKQLDGLLRDKTIWGVDLIEIGLSALVLSYFEKLIKGPGAVRQT"
     assembly_gap    1333..1432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1433..2634)
                     /locus_tag="PLF67_08685"
     CDS             complement(<1433..2634)
                     /locus_tag="PLF67_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOE90124.1"
                     /translation="MIYTRIEDKKGLTMEAAKAAFMEGLAKLGKRERVLLIPPDITRI
                     HGLGGMLAVWATEFYGNAVKQILPALGTHVPMTPKEIEVMYPGVDPSLFTVHDWRHDI
                     VTLGTVPKEFIEEVSEGKLSYEWKAQVNKALVHGGFDLILSLGQVVPHEVIGMANYNK
                     NVFVGTGGADGINKSHYLGAVYGMERIMGRTDSPVRAVLDYAEKHFAQELPLVYALTV
                     VGRNDHGEMKPMGLFVGDDRSCYEEAAALSLEVNFTMVPEPLEKVVVYLDPEEFRTTW
                     LGNKSIYRTRMAIADNGELIVLAPAVAGFGEDEEIDRLIRKYGYKGTPKVLSDVEKHK
                     DLKDNLSAAAHLIHGSSEGRFTIRYCPGKLTKEEIEQVGYEWGDLQQMLQRYPIESLK
                     DGWNTDAQG"
     gene            complement(2718..3656)
                     /locus_tag="PLF67_08690"
     CDS             complement(2718..3656)
                     /locus_tag="PLF67_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOE90125.1"
                     /translation="MNTYRFPPLTEFLIDQIAFAMEDQNTESLLDLKQGAVITDDERS
                     FLDEDEREDPHRFIELPEWTSADGFRLMEEYAEGVRNDAIRERLFFALNRGKGVFRAF
                     KDALAEEPTLERKWYLFKEQELRKQVILWYQREAGAAELEVLPPEDEELFEEILLEDF
                     TFEACELFDRKEIHRLVQTLLLEIGTFSGAGLLIEQALQNTERKLTHLARTATGELAG
                     LIVYTLVNDSTAEVLLYGIDVRYRGLGLFRLLFDGFSRQLSRLRVNRIIFGVLEQNPK
                     LKELFIRHGAAPIATAYELTTHQWNTNHANIEQAFL"
     gene            3769..5064
                     /gene="eno"
                     /locus_tag="PLF67_08695"
     CDS             3769..5064
                     /gene="eno"
                     /locus_tag="PLF67_08695"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOE90126.1"
                     /translation="MSIIEFIEAREILDSRGNPTVEVDVILEDGSMGRAAVPSGASTG
                     VHEAVELRDGDKSRYLGKGVLKAVDNVNTIIAPELEGLDALDQVGIDRAMIALDGTPN
                     KSKLGANAILGVSMAVARAAADHLSLPLFKYLGAFHANVLPVPMANILNGGAHSDNKV
                     DFQEFMVMPIGAPSFHEGLRMTAEVFHALKAVLSSRGLNTNVGDEGGFAPDLQSNEEA
                     LEVIMEAIKKAGYTAGRDGDFMIALDPAVSELYDSKTKTYTLKWTTGEKLTNAQMVDM
                     WEDWCNRYPIISIEDGMDEDDWEGWKLLTERIGDRVQLVGDDLFVTNSDRLKMGLEKG
                     VANSILIKVNQIGTLTETFEAIDLAKRNGYTAVVSHRSGETEDNFIADLVVALETGQI
                     KTGSMSRSDRLAKYNQLLRIEDYLDETAQYAGRGAFRVL"
     gene            complement(5428..>5646)
                     /locus_tag="PLF67_08700"
     CDS             complement(5428..>5646)
                     /locus_tag="PLF67_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90127.1"
                     /translation="RDDQKLQHGDMDQRTINMERVLPPGSTSSIERAVGVDSHLFHPG
                     AEGLIPVADNASPAIGSPMKPALETSSP"
BASE COUNT         1124 a         1586 c         1605 g         1231 t
ORIGIN      
        1 ggtctgccgg acggcgccgg ggcctttgat gagcttttca aagtaggaga gcaccagggc
       61 gcttaacccg atttcgatca ggtcgacgcc ccagatcgtc ttatctctca gcagtccgtc
      121 aagttgcttg aagggaccct tgtcgccaag cttgattccc ttcacatact cctgagcctc
      181 ttccagacgg ggatcggagc tgggggtgaa cggcctgccc tcatcgtcga tccccatcag
      241 gtaccgaagc cagccggcaa agaccagcgg aatcagtttg agatccttga tgtccagatc
      301 gggatgctca aggtagctct tgaccgtctc gccgaatcgg atcgcgagct tctggctcgt
      361 gtcggtggct atccgctggg ggctgtccgg catgaagggg ttggggaagc gcttgttcaa
      421 gacctcgtcg atgaactgcc tgggatcgat gatcccggga tcaaccacga ccggcatccc
      481 ttccgtatat ccgattcctt ccaccagcgc cctgagctgg ggatctttca tctcctcgct
      541 gatgagggtg aagcccagaa tcgaaccgaa gattgcaagg gcggtgtgca gggggttcag
      601 acaggtcgtg actttcatcc gttcaacctt gttcaccgtc gaacggtcgg tgaagtagac
      661 gcccaccttg tccaaaggag gcctgccgtt cttgaaggtg tcctcgatga ccagatattc
      721 ggcctcttcc gcgttgacga agaatgcgat gtgggcattg ttcttggtca cgatcaagcc
      781 gccgctttta aagccgtcct tcttgaggat ttcaacgatt ttcgcatccg ggcggggggt
      841 gatcttgtcg atcatcgacc agggataggc gacgttctca tccaggtagg cgaggaactc
      901 ctgctccaca tggccgcgct ccgcccagcc cttgccgatc gtcttgaccg cctcggcgat
      961 ccggtcgccg ttcgccgagc agttgtccat gctcaccagg gtcagattgc cgcccttttc
     1021 ccgataacga tggtaaagaa ggcttgtcag ctttgcaagg aaggattttg gcgcctttgc
     1081 tccttcctcg atgtccttgg cgatcggctc gaagagcgtg ccgtcgtttg aacggatagc
     1141 atatgctttc tcagtgatgg tgaagctcac catctgaagc gaggggttgg cgaatgttcg
     1201 tttgaagaac gcccagtctt cgggaaaggc cggttcgacc ggctttgcgg cggcgacgga
     1261 ggcgatgacc tccttgtcga ccgatccgtc gcttttgagg gtaaccgcca ggatcagatc
     1321 atcctgtttg gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccttgggc
     1441 gtcggtattc caaccatcct tcaggctctc gatcggatag cgctgaagca tctgttgcag
     1501 gtcgccccac tcatagccga cctgctcaat ctcttccttg gtgagcttgc cggggcaata
     1561 gcggatcgtg aaccgtccct cggacgatcc gtgaatcagg tgagcggccg cagaaaggtt
     1621 gtctttgagg tccttatgct tctcgacatc ggaaaggacc tttggcgttc ccttgtagcc
     1681 gtacttgcgg atcagccggt cgatctcttc atcttcgccg aagcctgcaa cggcaggggc
     1741 caggacgatg agttccccgt tgtcggcgat cgccattcgg gtgcggtaga tcgacttgtt
     1801 tccaagccac gtggtgcgga actcctcggg atcgaggtag acgaccacct tctcaagggg
     1861 ctcgggcacc atcgtgaagt tcacttccag ggagagtgcc gccgcctctt catagcagga
     1921 tcggtcgtcg ccgacaaaga ggcccatcgg cttcatttct ccatggtcgt tccgtcctac
     1981 gacagtaagc gcgtagacaa gcgggagctc ttgggcgaag tgtttttcag cgtagtcgag
     2041 caccgcccga accggggaat cggttcgtcc catgatccgt tccatcccgt agaccgcccc
     2101 caggtagtgg ctcttgttga ttccgtccgc cccgccggtg ccgacgaaga cgttcttgtt
     2161 gtagttcgcc atcccgatca cctcgtgcgg cactacctgg cccaacgaga gaatcagatc
     2221 aaagcctcca tggacgagcg ccttgttcac ctgggctttc cattcataag agagcttgcc
     2281 ctcgctgacc tcttcgatga actcctttgg taccgttccg agggtgacga tgtcatgtct
     2341 ccagtcatga accgtgaaga gggacggatc gacgccgggg tacatcacct caatctcttt
     2401 aggggtcatc ggcacatggg ttcccagagc gggaaggatc tgtttcacgg cgttgccgta
     2461 gaactcggtc gcccacacgg caagcatccc gcccaagccg tggatccggg tgatgtccgg
     2521 ggggatgagc aagacccgtt cccgcttgcc cagcttggca agcccttcca tgaacgcggc
     2581 cttggccgcc tccatggtca atcccttctt gtcctctatc cgtgtgtata tcatcgatac
     2641 ctccagtata tgcgagacag cgccttcatc gtaccatgaa ggcgatacgc ccgccaaggt
     2701 gaaagaatct cccctttcta gagaaacgcc tgttcgatgt tcgcgtgatt cgtgttccac
     2761 tgatgagtcg tcagttcata ggccgtcgca atcggggccg ctccgtggcg gatgaacagc
     2821 tctttcagct tcgggttttg ctcaaggaca ccgaagatga tccggttgac ccgaaggcga
     2881 ctcagctggc ggctgaagcc gtcaaacagg aggcggaaca agccgagccc ccggtagcgc
     2941 acatcaatgc cgtacaggag aacctcggcg gttgaatcgt ttacaagggt atagacgatg
     3001 aggccggcaa gctcgccggt ggcggtgcgg gcgagatggg tgagctttcg ttcggtgttt
     3061 tgaagagcct gttcgatgag aagacccgct ccgctaaagg ttccgatctc cagcagaagc
     3121 gtttggacaa gccggtggat ctctttccgg tcgaagagct cgcacgcctc gaaggtgaag
     3181 tcctcaagaa gaatctcttc aaacagttct tcgtcttcag gagggaggac ttccagttcg
     3241 gcggctcccg cttcccgttg gtaccagaga atgacttgct tccgaagctc ctgttccttg
     3301 aagaggtacc acttccgctc cagggtcggt tcttcggcaa gggcgtcctt gaacgcccgg
     3361 aacacccctt tccctcggtt gagggcaaaa aagagccgct ctcggatcgc atcgttgcga
     3421 acaccttcgg cgtactcttc catcaggcgg aagccgtcgg cgctggtcca ctcgggcaac
     3481 tcgatgaagc gatgaggatc ttcccgctca tcttcatcaa gaaaggagcg ctcgtcatcg
     3541 gtgatgactg cgccctgttt cagatccaag aggctctcgg tgttctgatc ctccatcgca
     3601 aaggcgattt gatcgatcag gaactcggtc aggggaggaa atcggtaggt attcatggtg
     3661 aaagtatact cccggggccg cttcccggca aggtcggcat tcttgacaca tatggcaaaa
     3721 gaacggaaac taatgaggtg aacgaattac aaacagagga gaagtttgat gagtattatt
     3781 gaatttattg aagccagaga gatcctggat tcccgtggaa accccactgt tgaagtagat
     3841 gtcatccttg aggatggatc catgggtcgg gccgcggtgc cctccggcgc atcgactggc
     3901 gtccacgaag ctgtcgagct tcgcgacggc gacaagagcc gctacctcgg taagggtgtt
     3961 ttgaaagctg ttgacaacgt caataccatc atcgctcccg agctggaagg ccttgatgcc
     4021 ttggatcagg tcggcatcga ccgggcgatg atcgccctcg acggaacccc gaacaagtcg
     4081 aagcttggcg caaacgccat tctgggcgtt tcgatggcag tcgcccgagc ggcggccgac
     4141 catttgagcc ttccgttgtt caagtatctc ggcgccttcc acgccaacgt gctgcccgtt
     4201 ccgatggcga acatcctcaa tggcggagcg cacagcgaca acaaggtcga cttccaggag
     4261 ttcatggtca tgccgatcgg cgctccctcc ttccatgaag gactccggat gaccgccgaa
     4321 gtgttccatg ccttgaaggc ggttctctct tccagagggt tgaacaccaa cgtcggcgac
     4381 gagggcggat tcgcccccga cctccaatcc aatgaagaag cccttgaagt gatcatggaa
     4441 gcgatcaaga aggccggata caccgccggc cgcgacggcg acttcatgat cgcccttgat
     4501 ccggcggtca gcgagctcta cgactcgaag accaagacct acaccttgaa gtggaccacc
     4561 ggcgagaagc tcaccaacgc ccagatggtc gacatgtggg aagactggtg caaccgctat
     4621 ccgatcatct ccatcgagga cggaatggac gaggatgact gggaagggtg gaagcttctg
     4681 accgagcgca tcggcgaccg cgtccagctc gtcggagacg acttgttcgt caccaacagc
     4741 gaccggctga agatgggtct tgagaagggg gtagccaact cgatcctgat caaggtgaac
     4801 cagatcggca cacttaccga gaccttcgaa gcgatcgatc ttgcgaagcg gaacggctac
     4861 accgcagtcg tctcccaccg ctcgggtgaa acggaagaca acttcattgc cgacttggtc
     4921 gtcgccctcg agaccggaca gatcaagacc ggttcgatga gccgcagcga ccgcctcgcc
     4981 aagtacaacc agctccttcg cattgaggat taccttgatg aaaccgctca atatgccggt
     5041 cggggcgcgt tccgcgtgct gtaagacggc aatactatca gacattcagg gccaccgcga
     5101 ggtggtcctt tctatgaaga gtggttgaat cggtgctatg ctttacgcat acacgtagga
     5161 atgggaaagc gatgagacgg tggtgagaag atgttggatg gatcgacgct ctggagtctg
     5221 caaggtcgct gcttcttctg attgccgcag gagcccttgt ccgaaggttg aagctcattg
     5281 acgaggaggg acaagccgcg ttgaccaacg tggttctctg tgtgacgctg tcgagttcct
     5341 gctctcggtc ggagcgcaga ttttcagctt caccctcttc aggctacact atcgaaatgg
     5401 tgaggagtcc aagcggaacg tgctccgcta cggactgctc gtttccaacg ccggcttcat
     5461 ggggctgccg atcgccgggc ttgcgttatc tgcgaccgga atcaatcctt cggcacccgg
     5521 atgaaacagg tggctgtcca ccccaaccgc tctttcgatg ctgcttgtcg agccgggggg
     5581 cagaaccctt tccatgttga tcgtccgctg atccatgtca ccgtgttgaa gcttctggtc
     5641 atcccg
//
LOCUS       DAOSLI010000085         5612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_12967_length_5612_cov_22.982545, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000085 DAOSLI010000000
VERSION     DAOSLI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12967_length_5612_cov_22.982545"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..527)
                     /locus_tag="PLF67_08705"
     CDS             complement(51..527)
                     /locus_tag="PLF67_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90128.1"
                     /translation="MKQTPEYDQIQAQMQKGVITLEGFLGDDPRKLVDIIASDTMTVH
                     NLNTTCALIAGKMEHFRDLGFEGLGEFITLDEHFEVKVDSVRGLLPSPFGGPGMYRKV
                     NTTVVNKTLGRQITYTDLHIHLIGDHCFFQGEGSTYRLEPKELVEILEVESLPSEE"
     gene            complement(539..1642)
                     /locus_tag="PLF67_08710"
     CDS             complement(539..1642)
                     /locus_tag="PLF67_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOE90129.1"
                     /translation="MIVSLVISVAVISLISVFLALLMVVADATIGNYGIVKVDINDGT
                     RKLEVKGGMPLLSTLTSQGVFIPSACGGRGSCGLCKVQVLSGVGDYLPTELPFISKEE
                     QAQNIRLSCQIKVKQNIAIKIPEELFNVRQYKTTVERIRDLTHDIKEVTLRLAPGETI
                     TPKAGQYIQFVVPEYGDLDESVYRAYSVASPPDDNTRVELEIRLVPNGICTTYVHTIL
                     KEGDEVTINGPYGDFYVRDTDRPMICIAGGSGMAPIKSILLDLKNKGSKRKVTYFFGA
                     RSRRDLFLLDEMFALEKELENFTFVPALSEPKEEDKWEGEVGLITDVVRRLSGDAANS
                     EAYLCGSPGMIDACIAVLTELHMPEEHIYYDKF"
     gene            complement(1653..2246)
                     /locus_tag="PLF67_08715"
     CDS             complement(1653..2246)
                     /locus_tag="PLF67_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOE90130.1"
                     /translation="MPSQVSAFAVFFASIFTSNILLTNYLGMCSFLAVSKEMKTSVGL
                     GAAVTFVLAVTTPLNWLVYHYLLIPFNLEYLRFIVFIIVIAAFVQLTEMTLERYSEPL
                     YQSLGIFLPLITVNCAILGASLFMVIREYTFITSLLFGLGSGIGWLLAIVAMSGIRTK
                     LRTANIPPALEGPGISLIIAGFMAMAFMGFSGMIAVS"
     gene            complement(2239..2850)
                     /locus_tag="PLF67_08720"
     CDS             complement(2239..2850)
                     /locus_tag="PLF67_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOE90131.1"
                     /translation="MAKSKLLKTFTEPLGKNNPVFVQILGICSTLAVTNKVQNTMVMV
                     LGVTFATALSAWTVSLLRKLIPSRIRMMVETMIIAAYVILVDIILKAYYPEMWKQLGP
                     YVGLIITNCIVMGRVEAFALTNKPLPSLVDGLASGLGYAYVLLLISVVRELMGSGSLW
                     GYRILGSWWTNWAIMIMPPGGFFILAIAIWMIRETVAKEAAHA"
     gene            complement(2853..3437)
                     /locus_tag="PLF67_08725"
     CDS             complement(2853..3437)
                     /locus_tag="PLF67_08725"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOE90132.1"
                     /translation="MSEFYKERIYPLLFMFITTVVCIVLTAGAHILTFDLAKANELSF
                     TRRAILDAAGLSYENTTLGIEEVYLASVTEKEGYYTAIVDGKTRYIIPLVGPGLWGTI
                     EIMAGLEEDLTTFSGVAIVNQSETPGLGGRIEEPWFTAQFAGKQGPFTLVEEGTAAQA
                     HEVDGITGASRTSEYFKNLTDRAASEARRIIQGV"
     gene            complement(3434..4393)
                     /locus_tag="PLF67_08730"
     CDS             complement(3434..4393)
                     /locus_tag="PLF67_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOE90133.1"
                     /translation="MKIQKQQAMRSVLYALAPLCLAAIYLFGWRFIAVLAVVVATAVT
                     SEYLMAKKYKMRITESLFVSATIFALSLPPTIPLWIAIVGMAFGIVFGKMVFGGFGKN
                     IFNPAITARAFIYISFGVPMTSKFVANHAGFPGGFASWLSVDVISAATPLVARDTGLL
                     NLFLGFNGGSFGETSALLIILGGLYIIYKKSANWKIVVSSLVGFLALQSILFFSGVQV
                     VTERGAELVASPLTALFSGSFLFAAFFMITDPVSAGQSTNLGRWIYGAAFGILTVLIR
                     SFSTWIEGVTFAILLVNMFAPLLDILIKDARAKKKAKQAGGKA"
     gene            complement(4456..5577)
                     /locus_tag="PLF67_08735"
     CDS             complement(4456..5577)
                     /locus_tag="PLF67_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90134.1"
                     /translation="MITIKPVVTRKEWNAFFAFPNDLYKGNKYFVPYLISDEKDTFTP
                     KKNPAHEYCDTQLFLAYKDGKVVGRIAGLINNKLNEMNKMKQMRFTRYDVIDDLEVSK
                     ALFEAVFAWGREKGMTEIIGPIGFSDFDKQGLLVEGFDQVGMFITLYNHPYYHDHLTK
                     LGFVKDVDWVEFKVYTPKELDPRVQRISEIAQKRHGYRLLEMKKKKEVIPYAKEIFHM
                     LNESYAHLYGFCPLTDGQIDMAIGQFFMVAQLDYIYVVVDKDDAVIGFGLMIPSLAKA
                     MQKSNGRLFPTGLLRILWSLKHYDVLDMYLIAVKPEYFGRGVNAVILHEGIKTAIRHG
                     VVYAETGPELETNTEVQSQWKGFETEQHKRRRCYKQALK"
BASE COUNT         1213 a         1537 c         1611 g         1251 t
ORIGIN      
        1 ggggacaatc atgccccggc cgtttggcat catcgtgctt tctgccttgc tcactcctcg
       61 cttggaagcg attctacttc aaggatttca accagctctt tcggctcaag gcggtaggtc
      121 gatccttcgc cttggaagaa gcagtggtcg ccgatgaggt ggatgtgcag gtcggtatag
      181 gtgatctggc gaccgagcgt cttgttcacc acggtcgtgt tcactttccg gtacatcccc
      241 gggcctccga agggcgaggg aagaagccct cggacggaat cgaccttgac ttcaaaatgc
      301 tcatccaagg tgatgaactc cccaaggcct tcaaaaccga gatcccgaaa gtgctccatc
      361 ttcccggcga tcaaagcgca ggtggtgttg aggttgtgaa ccgtcatcgt atcggaagcg
      421 atgatgtcca cgagcttgcg gggatcatcg ccgagaaatc cttcaagggt gatgacccct
      481 ttctgcatct gtgcctgaat ttgatcatat tcgggcgtct gcttcatgat ctcccccctt
      541 agaacttgtc atagtagatg tgctcttccg gcatgtgaag ctccgtaaga acagcgatac
      601 atgcatcgat catccccggg cttccgcaca ggtatgcctc gctgttggcc gcatcgccgc
      661 ttaagcggcg gacgacgtcg gtgatcaatc cgacttcgcc ttcccacttg tcttcctctt
      721 tgggttcgct cagcgcggga acaaaggtga agttctccag ctccttttcc aaggcgaaca
      781 tctcatcgag gaggaagagg tcgcgcctcg accgggcgcc gaagaagtag gttaccttcc
      841 gcttgctgcc tttgttcttc agatccagaa gaatcgattt gatcggggcc attcccgagc
      901 ctccggcgat gcagatcatc ggccggtcgg tatcgcggac gtagaagtcc ccgtacgggc
      961 cgttgatcgt aacctcgtct ccctccttca ggatggtgtg gacgtacgtc gtgcagatcc
     1021 cgttgggcac cagacggatc tccaactcga cccgggtgtt gtcatccggc ggggaggcga
     1081 ccgagtaggc ccgatagacg ctctcatcca gatcgccgta ttcgggaacg acgaactgga
     1141 tgtactggcc cgctttgggg gtgatggtct ccccgggggc cagccggagg gtgacctctt
     1201 tgatgtcgtg ggtgagatcg cggattcgct cgacggtggt cttatactgg cggacgttga
     1261 agagctcttc ggggatcttg atcgcgatgt tctgcttgac tttgatctga caggaaaggc
     1321 ggatgttctg agcctgctct tctttggaaa taaacggcaa ttccgtcggc aggtaatcgc
     1381 cgacaccgga gagcacctgc actttgcaga gtccgcatga gccccgtccg ccgcatgccg
     1441 aaggaatgaa caccccttgg gaggtgaggg tggaaaggag cggcatccct cctttcacct
     1501 caagcttcct ggtcccgtcg ttgatgtcca ccttcacgat tccgtagttt ccgatcgtcg
     1561 catcggcgac caccatcaac agcgccagaa acacgctgat gaggctgatg accgcgacgc
     1621 tgatgactaa tgaaacaatc atgcgatcct ccttaggaaa ccgcgatcat gccgctgaag
     1681 cccatgaatg ccatcgccat gaagccggcg atgatcagag agattcccgg tccctccaag
     1741 gcagggggga tgttggcggt tcgaagcttc gttcggatgc cgctcattgc cacgatcgcc
     1801 agcagccatc cgatgccgct gccgaggccg aagagcaggc tggtgatgaa cgtatactcg
     1861 cggatcacca tgaagaggct cgctccgagg atcgcgcagt tcacggtgat gaggggcagg
     1921 aagattccaa gtgattggta gaggggttca ctgtatcgct ccaaagtcat ctccgtcagt
     1981 tggacgaacg ccgctatgac gatgatgaag acgatgaagc gcaagtactc aaggttgaac
     2041 ggaatgagga ggtagtgata caccagccag ttcagcggcg tggtgaccgc cagcacgaac
     2101 gtcaccgccg ctccgagacc gacggaggtc ttcatctctt tggaaacagc aaggaaggaa
     2161 cacattccga ggtagttggt caacaggatg ttgctggtga aaatggaagc gaagaagacg
     2221 gcaaaggcgc ttacttgact aggcatgagc ggcctccttt gccacggtct cgcggatcat
     2281 ccagattgcg atcgcgagga tgaagaaacc gcccggcggc atgatcatga tcgcccagtt
     2341 ggtccaccac gagccgagaa tccgataacc ccagaggctc ccggatccca tcagctcccg
     2401 aaccaccgag ataagcagaa ggacgtaggc gtagccgagt ccgcttgcaa gtccgtccac
     2461 cagggagggc aggggcttgt tggtgagggc gaacgcttcg acccggccca tgacgatgca
     2521 gttggtgatg atcagtccga cgtacggtcc gagctgtttc cacatctcgg ggtagtatgc
     2581 cttgaggatg atgtccacca ggatcacgta ggcggcgatg atcatcgttt cgaccatcat
     2641 ccggatccgg gaaggaatga gctttctcaa gagggagacg gtccaggcgc tcaacgcggt
     2701 ggcaaaggtt actccaagaa ccattaccat cgtattttgt accttgttgg taaccgcgag
     2761 cgtcgaacag attcccagga tctgcacaaa gaccgggttg ttcttcccaa gcggttcggt
     2821 gaatgttttc aggagtttgc ttttggccat ccttacactc cttgtatgat gcgccgggct
     2881 tccgatgctg cccgatcggt cagattcttg aaatactcac tggttcgact tgcaccggtg
     2941 attccgtcaa cttcatgcgc ctgagcggct gttccctctt ccacgagggt gaacggtccc
     3001 tgtttgccgg caaactgggc ggtaaaccac ggctcctcga ttcggcctcc aaggccgggg
     3061 gtctccgact ggttgacgat tgcaaccccg ctgaaggtgg tcagatcctc ctccagcccg
     3121 gccatgatct cgatcgttcc ccacagtccc ggaccgacaa gggggatgat gtagcgggtc
     3181 tttccgtcga cgatggcggt gtagtagcct tccttctcgg tgaccgaggc gaggtagacc
     3241 tcttcgattc cgagggtcgt gttctcgtag gagagcccgg cggcatccag aatcgccctc
     3301 cgggtgaagg aaagctcgtt cgcttttgca aggtcgaagg tgaggatatg ggctcccgcg
     3361 gtcagcacga tgcacaccac cgtggtgatg aacatgaaga ggagcgggta aatccgttct
     3421 ttatagaatt cactcatgcc ttgcctccag cctgtttcgc tttctttttc gcccgagcgt
     3481 ccttgatgag gatatcgagt agcggagcga acatgttcac cagcaggatt gcgaacgtaa
     3541 ccccttcgat ccacgtggaa aagcttcgga tcaggacggt gaggattccg aaggccgctc
     3601 cgtagatcca ccgtccaagg ttcgtcgatt ggccggcgga gacggggtcg gtgatcatga
     3661 agaaggcggc aaagaggaag gatccgctga agagcgccgt aagcggggag gcgaccaatt
     3721 ccgctccgcg ctccgtcacc acctgtactc cactgaagaa gaggatgctc tggagggcca
     3781 ggaacccgac aagagaggaa acgacgatct tccagttcgc acttttcttg tagatgatgt
     3841 acagcccgcc gaggatgatg agcagggcgc tggtctcgcc gaaggagcct ccgttgaatc
     3901 ccaaaaacag gttcagaaga ccggtgtccc tggcgaccag cggggtcgcc gcgctgatca
     3961 cgtcaacgct cagccaggag gcgaaccctc cgggaaaccc ggcatggttg gcgacgaact
     4021 tgcttgtcat cgggacgccg aagctgatgt agatgaacgc ccgggcggtg atcgcggggt
     4081 tgaagatatt ctttccaaac ccgccaaaca ccattttgcc gaaaacgatt ccgaacgcca
     4141 tgccgacgat tgcaatccag aggggaatcg tcggcggcag gctgagggcg aagatggttg
     4201 ctgagacgaa gagcgactcc gtgatacgca tcttgtactt ttttgccatc aggtattcgc
     4261 ttgttaccgc agtggcaacc accaccgcca gcacggcgat gaagcgccat ccaaacagat
     4321 aaatggctgc aaggcagagc ggagcgaggg cataaagcac cgaacgcatc gcctgctgct
     4381 tttgtatctt cacaggattg tcctccctat tcggtcagta tacggcaaga aggggtatat
     4441 gtaaaaagaa ataccctact ttaatgcttg cttatagcaa cgacgtcgtt tatgctgctc
     4501 ggtttcaaag cctttccact gggattgaac ttccgtgttg gtttccaact cgggcccggt
     4561 ctccgcgtac acgacgccat gcctgatcgc cgtcttgatt ccttcatgca ggatgacggc
     4621 attcactcct cggccgaaat actccggctt gaccgcgatc aggtacatgt caagcacgtc
     4681 gtagtgcttc aaggaccaca ggatgcgcag cagtccggtc ggaaacagcc ggccgttgga
     4741 cttctgcatc gccttcgcaa gggaaggaat catcaacccg aagccgatga ccgcatcatc
     4801 tttgtccact acgacgtaga tgtaatcgag ctgagccacc atgaagaatt ggccgatcgc
     4861 catgtcgatc tggccgtcgg tgaggggaca gaacccgtag aggtgggcgt aggattcgtt
     4921 cagcatgtgg aagatctcct tggcgtaggg gatcacttcc tttttctttt tcatctccaa
     4981 cagccgatac ccatggcgct tctgggcgat ctcgctgatc cgctggactc gcggatcgag
     5041 ttccttgggg gtgtagacct tgaattcgac ccagtcgaca tccttcacga atccgagctt
     5101 cgtcaggtgg tcgtggtaat acgggtggtt gtagagggtg atgaacatcc cgacctggtc
     5161 gaacccctcg acgagcaggc cctgcttgtc aaagtcgctg aacccgatcg gtccgatgat
     5221 ttcggtcatc cccttttccc tgccccaggc aaagaccgct tcaaaaagcg ctttggagac
     5281 ctcaagatca tcgatcacat catagcgggt gaagcgcatc tgcttcatct tgttcatctc
     5341 gttgagcttg ttgttgatca gaccggcgat gcggccgacc accttgccat ccttgtaggc
     5401 cagaaagagc tgcgtatcgc aatactcgtg cgcggggttc tttttggggg tgaaggtatc
     5461 tttttcatcc gagatgagat agggaacaaa atacttgttg cctttgtaga gatcattggg
     5521 aaaagcgaaa aaagcattcc actcttttct ggtaacaacc ggcttgatgg taatcatagc
     5581 tatacactcc aagcgtattg tgtagttaac ct
//
LOCUS       DAOSLI010000086         5306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_13885_length_5306_cov_26.420301, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000086 DAOSLI010000000
VERSION     DAOSLI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13885_length_5306_cov_26.420301"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2691)
                     /locus_tag="PLF67_08740"
     CDS             complement(<1..2691)
                     /locus_tag="PLF67_08740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE90135.1"
                     /translation="MMHINFMVNHRVSDLNKRIMDANHLITELYTLEHIFQSIRLAQR
                     GHLLVGTDDYLEAFEIQNQAYVSTLSRIECVVKNDPEQHRLLQEIRNTYNATLIYHIT
                     PLFERRSVFEDDPSLIFKDDIIQDLLAHSSNASFTLQTLIGTLQEHERMSLEKAYDDF
                     SFWQYVNWVSIFLIPSVGVPLLFVGSFLFLRELHRYRTDQEKHLESLRLERDRYEEAI
                     RCSNLFSYSWDLENDVITPEDRFIRLLGYDPERIQGMTAIQYSQFFHPDDIGKLAIDY
                     QAHLMGKSEHLTTMVRMIHKDGHPITFMLRGQISRRSSDGEPLEIVGTYNDITEQVTL
                     ALAPKQDMEDLKAMFNTMDQGLAYMRLIHNEAGDVVDFEIVRANRAHSEFIGVPNEEI
                     LHRPASTLSPPLSKELVELNLNVGITGKSVVFETEDLVFGRYFRISSYQPEPGFVAML
                     MDDITNERDMEHKVLYERMLFETTLLSVAEGVISTDEVGIVQFINEAAEHLIGWEAEE
                     AIGRPLSEIFHLVPTDRRKRAPDLAQLVLSKRESLSLGDQATLVARDGVERYISDHAS
                     PIIGKDGELYGMVIVFRDATEDRTKAQEMRTLSISDPLTSLYNRRHFDRIKEEINNEP
                     YQPLTLVLADVNGLKLTNDAFGHDAGDELLRKVATVMKRTCRENDIIFRVGGDEFVLL
                     LPQTDAEHAETILNRINNALKKEKVRNLLISVAFGSAQHNTEEGFETTFKTAEDRMYR
                     NKLKENVTYKRQMIVSILDQLYEKEESIEEHNKLVSSYAKDLAMQTQLSSEEVELIEK
                     AALFHDLGKIAINKNILRLKTSQLTQVQTLELRRHPEIGYNILRSLDEYAPLAEAVLY
                     HHERFDGNGYPRGLKGEEIPLYARIIAVANAWANLTNPNST"
     gene            2942..3616
                     /locus_tag="PLF67_08745"
     CDS             2942..3616
                     /locus_tag="PLF67_08745"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOE90136.1"
                     /translation="MEEITKATGLPLIGDPAPAFRAVTTQGDINFPEDYKGKWVILFS
                     HPADFTPVCTTEFMTFASMHDEFKAMNTELVGLSIDSLYAHIAWLRKIQELEWKGMKN
                     IEVKFPVIEDIKMDVSRKYGMVQSQSSTQAVRAVFIIDPKGIVRTILFYPAAIGRNFD
                     EIKRAVQALQKADKDGVATPADWRPGQDVIIPTPGSCGTAKERMASQDEKMYCLDWFL
                     CFKKES"
     gene            complement(3653..4429)
                     /locus_tag="PLF67_08750"
     CDS             complement(3653..4429)
                     /locus_tag="PLF67_08750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE90137.1"
                     /translation="MTTYRKATWKEFYELKDLWNVVFSEEPAFLEKFFSTRFDPNHVF
                     VAEDEGRIVSALHSLPSSFTKEGITNPCSFIVGAATLQSHRNRGIMGGLLERVQASYP
                     HPITLFPALRPYYEKHGYFTASTARAYAIPPTLALNERQKRFATPDDLEPIYKSATYR
                     CGSLDRDELAWGFLIDGYETLYVEDGYAFILKDKAVEAFALNREAARLLLGILARRGI
                     STIQVVQDSPFESFLKDGCPVPMGMATDNALEGVYIAEQY"
     gene            complement(4426..>5306)
                     /locus_tag="PLF67_08755"
     CDS             complement(4426..>5306)
                     /locus_tag="PLF67_08755"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOE90138.1"
                     /translation="YIRGCMLEFYPPTLADKDLFPKHSRYLAYNYYYSYVHLWSQQIG
                     ITIAKNDTALYMHLRDDDCFLLPITDDLPKAMSELEEHSARTGLRFQLECVPESEALK
                     LQELGYSIQHVRNLDDYLYESSKLTKLSGRKLQAKRNHISQFERTYTYTVRSLSKPEM
                     RQECYEMASTRWLENHGEVDQSIINELRAIRRTFDNWDELGFVGMLVCVDNELTAFTV
                     GEIIDERLAIVHFEKGDTSYHGVYSVINQLFVSEFLRDVQYVNRQEDAGVDGLRKAKL
                     SYQPDLMVRKYRITKP"
BASE COUNT         1167 a         1400 c         1503 g         1236 t
ORIGIN      
        1 ggtggagttg gggttggtga ggtttgccca tgcattcgcc actgcgatga ttcgggcgta
       61 gagcgggatc tcctcgcctt tgagccctcg gggatagcca ttcccgtcaa accgctcgtg
      121 atgatagagc accgcctcgg caagcggggc gtattcatcc aatgagcgga ggatgttata
      181 cccgatctcg gggtggcgcc tcagctccag agtttgaacc tgcgtgagct gggaggtttt
      241 caggcggagg atgttcttat taatcgcgat cttccccaga tcatggaaga gggcggcttt
      301 ttcgatcagt tcgacctctt cactgcttag ttgggtctgc atcgccagat ccttggcata
      361 ggaggagacc agtttgttgt gctcctcgat cgactcctct ttttcataaa gctgatccaa
      421 gatggaaacg atcatctgcc gtttgtacgt gacattctcc ttgagcttgt tccggtacat
      481 ccggtcttcc gcggtcttga acgtcgtttc aaagccttct tcagtgttgt gctgagcgga
      541 accgaacgcc acgctgatga gcaggttccg caccttctct tttttcagcg cattgttgat
      601 ccgattgaga atcgtctcgg cgtgctcggc atcggtctgc ggcagcagga gaacgaactc
      661 gtcacctccc actcggaaga tgatgtcatt ctcccggcat gtccgcttca tcaccgtagc
      721 gactttcctc agcagctcgt caccggcatc atgaccgaac gcatcattgg tgagcttcag
      781 accgttcaca tcggcgagaa ccaaggtaag gggttggtat ggttcgttgt tgatctcttc
      841 tttgatccgg tcgaagtggc ggcggttgta gaggctggta agggggtcgg agatcgagag
      901 agtcctcatc tcctgcgctt tggttcgatc ttcggtggca tcgcggaaga cgatcaccat
      961 tccatacagc tcgccgtcct tgccgatgat cggtgaggcg tggtcggaaa tgtagcgctc
     1021 cactccgtct cgcgccacaa gggtcgcctg atcaccaagg ctcagggact cccgtttgga
     1081 gaggacgagc tgggcaagat ccggggcgcg ttttctccga tcggtcggaa ccaaatggaa
     1141 gatttccgaa aggggacgcc cgatcgcctc ttccgcttcc cagccgatca gatgttccgc
     1201 cgcctcattg atgaattgga cgattcccac ctcatcggtc gagatcactc cctcggcaac
     1261 gcttaagagg gtcgtttcaa agagcatccg ttcatacagc accttgtgct ccatgtcgcg
     1321 ctcgttggtg atgtcatcca tcagcatcgc cacgaacccc ggttcgggct gataggaaga
     1381 gatcctgaag tatcggccga acacaaggtc ctccgtctcg aagacgacgc tcttaccagt
     1441 aatcccgacg ttgaggttga gctcgaccag ttccttggaa agcggcggtg acaaggtcga
     1501 cgccggtcgg tgcagaatct cctcgttcgg cacaccgatg aactccgagt gcgccctgtt
     1561 cgcccgaacg atctcaaaat cgaccacatc tcccgcctca ttatggatca gccgcatgta
     1621 ggccaacccc tgatccatcg tgttgaacat ggccttgagg tcttccatat cctgtttggg
     1681 agccagggcg agggtgacct gttcggtgat gtcgttgtag gtgccgacga tctcaagggg
     1741 ctcgccatcg cttgagcggc gcgagatctg tccccggagc atgaacgtga tcggatgccc
     1801 gtccttgtgg atcattcgga ccatggtggt caggtgctcg ctcttgccca tgaggtgggc
     1861 ctgataatcg atcgccagct ttccgatgtc atcggggtgg aagaactggg aatactgtat
     1921 tgccgtcata ccctggatcc gctcggggtc atagccgagc agcctgatga atcgatcttc
     1981 cggtgtgatg acgtcgtttt caagatccca actgtaggaa aagaggttgg agcatcgaat
     2041 cgcttcttca tagcggtcgc gctcaaggcg gagcgactcc agatgttttt cctgatcggt
     2101 acgatagcgg tgcagttccc taaggaaaag aaaacttccg acaaacagca gcgggacgcc
     2161 tacggaggga ataaggaaga tgcttaccca gtttacatac tgccaaaatg aaaaatcgtc
     2221 atatgccttt tcaaggctca tccgctcgtg ttcctgaagc gtcccgataa gagtttgcag
     2281 cgtaaatgaa gcgttgcttg aatgagcaag caaatcttga ataatgtcgt ctttgaagat
     2341 gagcgagggg tcgtcttcaa agaccgaccg gcgctcgaac agcggagtga tatggtaaat
     2401 aagcgttgca ttgtaggtgt ttcggatttc ctgaagcagg cggtgttgct ccggatcatt
     2461 cttgaccacg cattcaattc tcgaaagtgt ggagacgtat gcctgattct ggatctcaaa
     2521 cgcttcgagg taatcatccg ttcccacaag aaggtgtccg cgctgggcaa gccggatcga
     2581 ttgaaagata tgttccaatg tatacagctc cgtgatcaga tgattggcat ccataatccg
     2641 cttgttgagg tcgctgactc gatgattcac catgaagttg atatgcatca tgaccgccag
     2701 tgcaagaagg actataacga tgccaatgac aaaaagctga acaaagcgcg gttgcttttt
     2761 aatgataatt ccccctctcc tcattatagc aagagacgtc gctaaaacaa gaaaaaaaat
     2821 atataccccc atgcgatacc gaaaatcaaa aggagtatcg acctggaggg agaacgtatt
     2881 tgcctttcgt aatgataatt ggtatcatta aggtatcagt attactatga ggagtaaaac
     2941 catggaagaa atcactaaag caaccggttt gcccttgatc ggcgaccccg ctccggcgtt
     3001 ccgggcggtt accacccagg gagacatcaa ctttccggag gattacaagg gaaagtgggt
     3061 catcctgttc agtcaccccg ccgacttcac cccggtgtgc accaccgagt tcatgacctt
     3121 cgcctcgatg cacgatgagt tcaaggcgat gaacaccgag ctcgtcggtc tttcgatcga
     3181 ttcgctgtac gctcacatcg cgtggctgcg aaagattcag gagctcgaat ggaaaggaat
     3241 gaagaacatc gaagtgaagt tcccggtgat cgaggacatc aagatggatg tttcccgcaa
     3301 gtacgggatg gttcagagcc agagcagcac tcaggcggtt cgggcggtct tcatcatcga
     3361 cccgaagggc atcgtccgga cgattctctt ctatcccgcc gcgatcggcc ggaacttcga
     3421 tgagatcaag cgggcggtcc aggcgcttca gaaagcggac aaggatggcg tagccacccc
     3481 ggcggactgg agacccggtc aggatgtcat cattccgacc cccggctcct gcggcaccgc
     3541 aaaggagcgg atggccagcc aagacgagaa gatgtactgt ctggactggt tcctctgttt
     3601 caagaaggaa tcgtaaggat cacggaaaag ggggagcttt caccggctcc ctctaatatt
     3661 gctcagcgat gtagacgccc tcaagggcat tatcggtagc catgcccatc ggaaccgggc
     3721 agccgtcctt caaaaaggat tcaaacggtg agtcctgtac tacttgaatc gttgagattc
     3781 cccgtcgggc aaggatgcct aagagcaggc gggccgcttc cctgttcaac gcgaatgctt
     3841 cgaccgcctt gtccttgagg atgaacgcat agccgtcttc aacatagagc gtctcatacc
     3901 cgtcgatgag aaacccccac gccagctcat cccggtcgag cgagccgcag cggtacgtcg
     3961 cgcttttata gatcggttcc aggtcgtccg gggtggcgaa ccgcttttgg cgctcgttca
     4021 acgccaaggt gggcggaatc gcatacgccc gggcggtcga cgcagtgaaa tacccatgct
     4081 tctcatagta gggccggagg gcgggaaaga gggtgatcgg atggggatag gaagcttgaa
     4141 cacgctccag cagcccgccc atgattcccc ggttccgatg ggattgaagc gtcgctgctc
     4201 cgacgatgaa ggagcagggg ttcgtaattc cctctttcgt aaaggagctt ggaagggagt
     4261 ggagggcgga aacgatcctt ccctcatctt ccgccacgaa gacatggttc ggatcgaagc
     4321 gggtcgaaaa gaacttctca agaaaagccg gctcctcgct gaagacgacg ttccacagat
     4381 ctttgagctc ataaaactcc ttccaagtcg cttttcgata ggtggtcatg gcttcgttat
     4441 ccgatatttc ctgaccatca ggtccggctg gtaggagagc ttcgctttcc tcagtccgtc
     4501 gacaccggca tcctcctggc ggttgacgta ttggacatcc cggaggaact cggagacgaa
     4561 gagctggttg atcaccgaat acacgccgtg gtaggaggtg tcgccctttt cgaagtggac
     4621 gatcgccagt cgttcgtcga tgatctcccc gacggtgaag gcggtcagct cgttgtccac
     4681 gcagaccagc attccgacga acccgagctc gtcccagttg tcgaaggtcc gccggatcgc
     4741 cctcagttcg ttgatgatgc tttggtcgac ctctccgtgg ttctccagcc accgggtgga
     4801 ggccatctca tagcactctt gtcgcatctc cggtttggaa agggagcgca ccgtataggt
     4861 gtaggtccgt tcgaactggg agatgtggtt ccgcttcgcc tgaagcttcc gcccggagag
     4921 cttcgtcaat ttcgatgatt catagaggta gtcgtcgagg ttgcggacat gctggatcga
     4981 gtagccgagc tcctgcagct tgagcgcttc gctttcggga acgcattcca gctgaaagcg
     5041 aaggccggtt cgggcgctgt gctcttccag ctcgctcatc gccttgggca gatcgtcggt
     5101 gatgggaaga agaaaacaat cgtcgtcccg aaggtgcata tagagggcgg tatcgttctt
     5161 tgcgatcgtg attccgatct gctggctcca gaggtgaacg taggagtagt aatagttgta
     5221 cgccagatag cggctgtgct tcggaaacag gtctttatcg gcaagggtag ggggatagaa
     5281 ctctagcata catcctcgaa tatatc
//
LOCUS       DAOSLI010000087         5301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_13903_length_5301_cov_27.550896, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000087 DAOSLI010000000
VERSION     DAOSLI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13903_length_5301_cov_27.550896"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLF67_08760"
     CDS             complement(<1..1192)
                     /locus_tag="PLF67_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOE90139.1"
                     /translation="MINAALQKLWESTGLYGFFGLAEGFGWGNFAMILVGFVLLYLAI
                     KKGFEPLLLIPIGFGCILSNIPLAYISGMDPATGDLGFIKILFDMGIQSGLFPILIFM
                     GVGAMTDFGPLIANPKTFLLGAAAQLGIFAALIGALAISFIPGINFTLHDAAAIGIIG
                     GADGPTAIYVTSRLAPNLLGPIAVAAYSYMALVPIIQPPIMRALTTEEERKIKMKQLR
                     PVSRLEKILFPLSVLTMCAILLPSATPLVGALMFGNLAREVGVINRLSDTMQNALMNI
                     VTIFLGLAVGSKMEARLFLNLNTLGILFLGMVAFSLGTAGGVLIAKLMNKIDKKHPIN
                     PLIGSAGVSAVPMAARVSSKIGQEADPQNFLLMHAMGPNVSGVIGSAVAAGVLLAVVP
                     FMEVL"
     gene            complement(1204..3024)
                     /locus_tag="PLF67_08765"
     CDS             complement(1204..3024)
                     /locus_tag="PLF67_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
                     /protein_id="HOE90140.1"
                     /translation="MKKVNFMVTAFRDGFQSVLGARVFTKDFMPAVEAAREAGITHYE
                     AGGGARFQTLYFYSNEDAFEMMDEFRRVAGPDADLQTLSRGVNVVGLDSQPRDIIKLH
                     AKLFKKHGISTIRNFDALNDVNNLIDSGQAIVDAGLRHEVVVTMMALPPGLKGAHDPD
                     FYEATLRKIMDAGIKYDSVAFKDASGTTTPAVVHETIKRARKLLGKDMNIVFHSHDTA
                     GICIQQYVSALEAGANQVDLSMAPVSGGTCQPDVLTMWHALRGTEFDLGIDIKKVREA
                     EAVFQDVMKDYILPPEAMTVSPEIIFSPLPGGALTTNTQMLRDNNLMDKFPEIVEAMA
                     ETVAKGGFGTSVTPVSQFYFQQAFNNVMFGPWKRIAEGYGKMVLGYFGKTPVEPDKEV
                     VKACADALGLEPTTEKVVDINDKDPSKGVEAAKAMLKKENLPTTDENIFITATCKEKG
                     ILYLTGKAQVNGMYKYDREAEAAKAKGEYTVTVNGKAYGVKLGKDSATVNGATYPISV
                     EYGINEGTIAESVAAAPVAAAPVAGSGPAIPVEAPMPGLVLRIDVKVGQSVKKNELLM
                     VMEAMKMENEIYAPADGVVTKISVSQGQQLQSGDELMVIG"
     gene            complement(3046..3315)
                     /locus_tag="PLF67_08770"
     CDS             complement(3046..3315)
                     /locus_tag="PLF67_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOE90141.1"
                     /translation="MVFLPQFPKEQLLTQLSNGFVLLVLGMGTVFIFLILLVFLTKGL
                     SAFVMKLESKRPAVATGKPAAAVAATASEADIAAAVAAAWAKANE"
     gene            3505..4356
                     /locus_tag="PLF67_08775"
     CDS             3505..4356
                     /locus_tag="PLF67_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOE90142.1"
                     /translation="MSDGMDFAARIAEDKAIRTNLDRIGKKILIMSGKGGVGKTTVTI
                     NLANALVDSGKRVGILDTDLHGPNVAKMLGCEDGILFSENEGKTFYPVEARPNLLVMS
                     LAFAIKDPDSPIVWRGPMKTGAIRQFLAQAEWGTLDYLLIDSPPGTGDEQLTVVQTIR
                     ELTGTIIVTTPQEVALLDARRSVAFSRTMGVAILGLIENMSGLFCPHCKEEIPVFGTG
                     GGERLASELGVPFLGRIPLEVPLMEAEDSGRSHLENAPESRSALALKMIALKIDQGTA
                     VSSIIKE"
     gene            4359..4931
                     /locus_tag="PLF67_08780"
     CDS             4359..4931
                     /locus_tag="PLF67_08780"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOE90143.1"
                     /translation="MHTHLIWKSGKREILYKGPIFDVAKVERTSTDGRCAPFIEVAAP
                     KWVTIIPVYRNKEGMPMVVMVDQFRHGSATVTREFPAGVVDEGETPKEAAVRELLEET
                     GLVGSRVTLLGEINPNPAFMSNRSYFYLIEDVVPQAKQDLDENEQIDVLSVPLEEVVE
                     QMGTGIYDNGIMMIALGFFMREAKRRRMTI"
     gene            4928..>5301
                     /locus_tag="PLF67_08785"
     CDS             4928..>5301
                     /locus_tag="PLF67_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOE90144.1"
                     /translation="MKKYLIIITIIAILTFTGCRSTGNSITRTIEVSASASVTVEPDI
                     ASFSVRVSEKGETTSEAQHKANRKMHALLSTLKAANIEERDLKTTMVSLWPNYEYIDN
                     RQVITGQVASQSVHVTVRNLSAL"
BASE COUNT         1065 a         1454 c         1682 g         1100 t
ORIGIN      
        1 ccaatacttc catgaaggga acgacggcca gaagcacgcc ggccgccacg gccgatccga
       61 tgactcccga gacgttcggt cccatcgcgt gcatcaggag gaagttctgg ggatccgctt
      121 cctgaccgat cttcgatgat acacgagcgg ccatcggaac ggcggagact ccggcgcttc
      181 cgatcagggg gttgatcggg tgctttttgt cgatcttgtt catcaatttt gcgatcagca
      241 cgccgccggc ggtgccgagg ctgaaggcga ccattccgag gaacaggatg ccgagggtgt
      301 tgaggttgag gaagagccgg gcttccatct tcgatccgac cgcaaggccc aagaagattg
      361 ttacgatgtt catcaacgcg ttctgcatcg tgtcgctgag gcgattgatc accccgactt
      421 cccgggcgag gttgccgaac atcagcgccc cgacgagcgg ggtcgccgag ggaagcagaa
      481 tcgcgcacat cgtcaacacg cttaagggga agaggatctt ctccagcctg gagacgggtc
      541 gcagctgctt catcttgatc ttccgttcct cttccgtggt cagcgcccgc atgatcgggg
      601 gctggatgat cggaacaagg gccatgtagc tgtacgctgc cacggcgatc ggtccgagaa
      661 ggttcggagc cagacggctg gtgacgtaga tcgccgtcgg tccgtccgct cctccgatga
      721 tgccaatcgc cgctgcgtcg tgcagggtga agttgattcc cggaatgaag ctgatcgcca
      781 gcgccccgat gagggcggcg aagattccca gctgggcggc ggcgccgagg aggaaggtct
      841 tcgggttcgc gatgagcggt ccgaagtccg tcatcgcccc gacgcccatg aagatgagaa
      901 tcgggaagag ccctgattga atacccatgt cgaagaggat cttgatgaat ccgaggtcgc
      961 cggtggcggg atccatgccg gagatgtagg ccaaggggat attggagaga atacatccga
     1021 atccgatggg aatcagcaga agcggctcga accccttctt gatcgccaga tagagaagga
     1081 cgaatcccac gaggatcatg gcgaagttgc cccagccgaa tccctcggcc aagccgaaaa
     1141 agccatagag tccggtggac tcccacagtt tttgtaatgc agcgttaatc attcctcact
     1201 cccttacccg atgaccatca gctcgtcacc gctttggagc tgctgacctt gggagacgga
     1261 gatcttcgtg accacgccat cggcgggagc gtagatctcg ttctccatct tcatcgcttc
     1321 catgaccatc agaagctcgt ttttcttcac gctctggccc accttcacgt cgatgcgcag
     1381 gacaaggccg ggcatcgggg cttcgacggg aatcgcaggt ccggaaccgg cgaccggggc
     1441 tgctgcgacc ggggcggccg caacgctctc ggcgattgtc ccttcattga tgccgtactc
     1501 cacggaaatg ggataggtgg ctccgttgac cgtcgcgctg tccttgccga gcttcacgcc
     1561 gtacgccttg ccgttgacgg tgacggtgta ttcgcccttc gctttggcgg cttccgcttc
     1621 gcggtcgtac ttgtacatcc cgttcacctg agcttttccg gtgagataga ggattccctt
     1681 ctccttgcag gtggcggtga tgaagatgtt ctcgtcggtg gtgggcaggt tctctttctt
     1741 cagcatcgct ttggcggcct cgactccctt ggagggatcc ttgtcgttga tgtcgacgac
     1801 cttctcggtg gtcggctcaa gaccgagggc gtccgcacac gctttgacga cctccttgtc
     1861 gggttcgacg ggggtcttgc cgaagtagcc gaggaccatc tttccatacc cctcggcgat
     1921 ccgcttccac ggtccgaaca tgacgttgtt gaacgcctgc tggaagtaga actgcgaaac
     1981 gggggtgacg ctggttccga agccgccctt ggcgacggtc tcggccatcg cttcgacgat
     2041 ctcggggaac ttgtccatca aattgttgtc ccggagcatc tgggtgttgg tggtcaacgc
     2101 gccgcccggc agcgggctga agatgatttc agggctgacg gtcatcgcct cggggggcag
     2161 gatgtagtct ttcatcacgt cctggaagac cgcttccgct tcgcggacct tcttgatgtc
     2221 gatgccgagg tcgaactcgg tgccgcgaag ggcgtgccac atcgtgagga catccggctg
     2281 gcaggttccg ccggagacgg gggccatcga gaggtcgacc tggttcgctc ccgcttccag
     2341 ggcgctgacg tactgctgga tgcagatccc ggcggtgtcg tggctgtgga agacgatgtt
     2401 catgtccttt ccgaggagct tgcgggctcg cttgatcgtc tcgtggacga ccgccggagt
     2461 ggtggttccg ctggcgtcct tgaaggcgac ggagtcgtat ttgatgcccg catccatgat
     2521 cttgcgaagg gttgcttcgt agaagtcggg atcgtgcgcc cctttcaacc cggggggcag
     2581 ggccatcatc gtcaccacga cttcgtggcg aaggccggca tcgacgatcg cctggccgct
     2641 gtcgatcagg ttgttcacat cgttcagggc gtcgaagttg cggatcgtgg agatcccgtg
     2701 cttcttgaag agcttggcat gaagcttgat gatatcgcgc ggctgggagt cgagcccgac
     2761 gacgttcact cctcgggaga gggtctgaag gtcggcatcg ggaccggcga cgcggcggaa
     2821 ttcgtccatc atctcaaagg cgtcttcgtt gctgtagaag tagagggtct ggaagcgggc
     2881 gcctccgcct gcttcatagt gggtgattcc cgcttcgcgg gcggcttcga cggccggcat
     2941 gaaatccttg gtgaaaacgc gggctcccag gacggattgg aagccgtcgc ggaacgcagt
     3001 gaccatgaag tttacttttt tcatctcaaa tgatcctccg gggggttatt cgttcgcctt
     3061 cgcccatgct gctgctacgg cggcggcgat atccgcctca gaggcggtgg cggcaacggc
     3121 tgctgcaggt ttgccggtgg caacggcagg ccgcttcgat tcgagtttca tgacgaaggc
     3181 cgaaagacct ttggtcagga aaaccagcag aatgagaaag atgaacaccg tccccattcc
     3241 aagaaccaac aagacgaatc cgttggataa ttgggtgagc agttgttctt tcgggaattg
     3301 aggtaagaaa accatgtgaa ctcctcgtat ggtatggtag cctcagcaca ggactgggaa
     3361 agtcgcattg gataccagta tatcgaaaga agctaaggcg tttcaagcaa tggcaggata
     3421 aatgattaag cgctgtaaaa cgggtctcct tcttgtcgct ttacccgtct tttcgtacat
     3481 tagactgata atggagcaac ctgtatgtcg gatggaatgg attttgccgc ccggatcgcc
     3541 gaggacaagg cgattcggac gaacctggac aggatcggga aaaagattct gatcatgagc
     3601 ggaaagggcg gggtgggaaa gaccaccgtc acgatcaacc tggcgaacgc cctggtcgat
     3661 agcggaaagc gggtcggtat cctggacacc gacctccacg gtccgaacgt ggcgaagatg
     3721 ctggggtgcg aggatgggat cctcttcagc gagaacgagg gaaaaacctt ttaccctgtt
     3781 gaagcccggc caaaccttct ggtgatgagc ctggcgttcg cgatcaagga tcccgactcc
     3841 ccgatcgtct ggcgggggcc gatgaagacc ggcgcgatcc ggcagttcct cgctcaggcg
     3901 gagtggggga cattggacta cttgttgata gactccccgc cgggaaccgg ggacgagcag
     3961 ctgacggtcg tccagacgat ccgggagctc accgggacga tcatcgtcac gacccctcag
     4021 gaggtcgccc tcttggacgc ccgccgctcg gtcgccttct cccggacgat gggggtggcg
     4081 atcctcggcc tgattgaaaa catgagcggg ctcttctgtc cgcactgcaa agaggagatt
     4141 ccggtgttcg gaacgggcgg cggcgagcgc ttggcaagcg agctgggcgt gccgtttttg
     4201 ggaaggatcc ccctcgaggt tcccctgatg gaggcggaag acagcggaag aagccatctt
     4261 gagaacgccc ccgagtcccg aagcgccctg gcgcttaaga tgatcgccct gaaaatcgac
     4321 caaggcaccg cggtgtcttc tattataaag gagtgactgt gcatacccac cttatctgga
     4381 aaagcggaaa gcgggagatc ctctacaagg gcccgatctt cgatgttgcc aaggtggagc
     4441 ggacctccac cgacggaagg tgcgctccgt tcatcgaggt cgccgccccc aaatgggtca
     4501 cgatcattcc ggtttaccga aacaaagagg gaatgccgat ggtggtcatg gtcgatcagt
     4561 tccggcacgg gtctgcgacc gtcacccggg agttccccgc aggcgtcgtg gatgaggggg
     4621 agacgccgaa ggaggcggcg gtgcgggagc tgctggaaga gacgggcctc gtcggttcaa
     4681 gggttacgct gcttggggag atcaatccca atccggcgtt catgagcaac cgctcctact
     4741 tctatttaat agaagatgtc gtcccccaag cgaagcagga ccttgatgag aatgagcaga
     4801 tcgatgtcct gtccgttccg cttgaggaag tggttgaaca gatgggtacg ggaatctatg
     4861 ataacggtat catgatgatc gccctcggct tcttcatgcg ggaggccaag aggcggagga
     4921 tgacgatatg aagaagtacc ttattataat aaccatcatc gcgatcctga cattcaccgg
     4981 gtgccgatcg acaggcaatt cgatcacccg gacgatcgag gtgtccgcaa gtgcctcggt
     5041 cacggtggag ccggacatcg cttcgttctc ggttcgggtg agcgagaagg gggagacgac
     5101 gagcgaggct cagcacaagg caaatcggaa gatgcatgcg ctcctttcga cgctgaaagc
     5161 ggcaaacatt gaagagaggg atctgaagac cacgatggtc agtctgtggc cgaactacga
     5221 gtacatcgac aacaggcagg tgatcaccgg ccaggtcgct tcccagtcgg tccatgtgac
     5281 ggttcggaat ctctcggctc t
//
LOCUS       DAOSLI010000088         5144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_14454_length_5144_cov_25.088230, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000088 DAOSLI010000000
VERSION     DAOSLI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14454_length_5144_cov_25.088230"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLF67_08790"
     CDS             complement(<1..770)
                     /locus_tag="PLF67_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE90145.1"
                     /translation="MVYSANEQRYEKMQYARTGNSGLKLPRISLGLWHNYGDAYPISN
                     GRAMLRTAFDLGINHFDLANNYGPPAGSAEENFGKILKQDFAPYRDELIISTKAGYYM
                     WPGPYGEWGSRKYLLSSLDASLKRMGLEYVDIFYSHRFDPNTPLEETMGALASAYRSG
                     KALYVGISSYTAEQTRKAYAILKDMDVPLLIHQPSYSMLNRWVEPELLSTLEELGVGA
                     ICFSPLGQGMLTDKYLKEIPSTSRAAKDLSLKKSMLSK"
     gene            complement(831..1454)
                     /locus_tag="PLF67_08795"
     CDS             complement(831..1454)
                     /locus_tag="PLF67_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90146.1"
                     /translation="MHRVIVLKRLDELTVVDDGTIYHGFDQEWYSKLWQRTAGCGPTT
                     ASTLIRYNAKRNKSSTKASSIRLMEELWSFVTPGIMGVHTVAHFTRGLREYLVKKHLG
                     FEIAALSVPIDRANRPSFAQVREFLVSRLEADQPVAFLNLNNGEVGNLDPWHWVTIVG
                     IEQRGADRPLLADVYDEGRKLVINLTLWYETTTRPGGFVSLVEGGEG"
     gene            complement(1465..1839)
                     /locus_tag="PLF67_08800"
     CDS             complement(1465..1839)
                     /locus_tag="PLF67_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE90147.1"
                     /translation="MNRIILYALILMILFLIVKKVGGGARISAKDVYALLQSGEEVIL
                     VDVRTEGEYYSGHIDGAIHLSLYDIGAEKPALLSDLDAPIIVYCQSGARSGIARRKLK
                     ALGYTNVRDMGGLMFWRYDLVK"
     gene            complement(1926..>2353)
                     /locus_tag="PLF67_08805"
     CDS             complement(1926..>2353)
                     /locus_tag="PLF67_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOE90148.1"
                     /translation="KRTRTTYYAVASAKQSTEALSALRAESAAKDMIGRWAETSIDAS
                     VVTFIEEAGETLRTRQILEVLQNLSVQTVNIALRGVDIVDRWVRDDGTVYVLASYPVK
                     NLKEAYRLQAEELERKALADAIKADVLETYLQAQLEEAD"
     assembly_gap    2354..2453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2617..3207)
                     /locus_tag="PLF67_08810"
     CDS             complement(2617..3207)
                     /locus_tag="PLF67_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90149.1"
                     /translation="MKKIVLLVAMATILMVGTSCATSKKAAVVTPPVPAAKVVVGAEG
                     FIQPDWVYKTPTAGDRHFESGYGLMSNRQNSIKRAETEARNKIAGWISTNVQEVVVTY
                     LNDAGSLGDRQALDAMEVISKQVAEATLVGVTTEDVWVDKEGGVWVLASIPLANIQYA
                     FEPAAEAVVETFTPSAAAEAANAKMKEAFAKLLKGD"
     gene            complement(3226..4176)
                     /locus_tag="PLF67_08815"
     CDS             complement(3226..4176)
                     /locus_tag="PLF67_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90150.1"
                     /translation="MRKGILLFVLIPFLVSCMSIPSQRPAWIGRPYDGKYPEDKYLLV
                     VGSGSSYQKAVENARINLASVFSSQVEAVTRITTIDSSEGGSFDALFEQGTVSSNVED
                     LVGSEVVSSFTDEMGQTYVRLAVERQESARFLQTRLAELTKELAKIRSQTSADRLERY
                     RFLLSAYPVAQEAGQIADRIWVLTKEINPDHLGALASELRTLARSISVEVIVHSETDP
                     HFLKAGFEGALQNLGFVQGGGDYALTVTYDSEPILFEASPYAYERYQLSVTLGRNDKV
                     VRSFSLSERIAALHQGDAKTKALQSALNDGIETLIGLLSQ"
     gene            complement(4189..4806)
                     /locus_tag="PLF67_08820"
     CDS             complement(4189..4806)
                     /locus_tag="PLF67_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HOE90151.1"
                     /translation="MRRSRNIIISIIMLLSLVFLTACQSSYSVSRVSSDKDLDLSGSW
                     NDTDIRLVSEALVQSSMKGAWINNFRMKNLGRNPVVIVGTFINRSSEHIDTAIIAKRY
                     EMELVNSGKVDMVADQNFRASIRQEREEQQYFASEETAKALGREIGADFLLQGAVRSV
                     LDQQGNTRVRTYYVSAELIDIETSRKVWVGEETIKKVIQQARYSL"
BASE COUNT         1091 a         1454 c         1283 g         1216 t
ORIGIN      
        1 tccttgctga gcatcgactt cttcaaggaa aggtctttcg ccgcccgact ggtcgatgga
       61 atctccttaa ggtacttgtc ggtgagcatc ccttggccaa gaggtgagaa acagatcgcc
      121 ccgaccccca gctcctccaa ggtggaaaga agctcgggct cgacccatcg gttcaacatc
      181 gagtaggagg gttgatggat caacaaagga acatccatat ctttcagaat agcgtatgct
      241 tttcgagtct gttccgctgt gtaggaggag atccccacat aaagcgcctt gccgctccta
      301 tacgcactgg ccaaagcccc catcgtctcc tccaagggag tattgggatc gaagcggtgg
      361 gagtagaaga tatccacgta ctcaagcccc atccgtttca agcttgcatc cagacttgaa
      421 agaaggtatt tccggctccc ccactccccg taaggacctg gccacatata gtatccggct
      481 ttcgtggaga tgataagctc atcccgatac ggggcgaagt cctgtttcaa gatcttgcca
      541 aagttctcct ccgccgagcc ggcgggcggt ccgtagttgt tggccaggtc gaagtggttg
      601 atccccagat caaaagcggt tcgaagcatc gccctaccgt tggaaatcgg gtatgcatca
      661 ccgtagttgt gccaaagtcc gagcgaaatc cggggaagct tgagcccgct gtttcctgtt
      721 cgagcgtact gcatcttttc atatcgttgc tcatttgctg aatataccat gactgttctc
      781 cttctctgtc agtatacacc accgaaaaaa cgggagctac cgcagctcct tcaaccttcg
      841 ccgccctcca cgaggctgac gaatcccccc gggcgggtgg tcgtctcata ccagagggtg
      901 agattgatca cgagctttct tccctcgtcg tacacgtcgg caagaagcgg tctatccgcc
      961 ccccgctgtt cgatccccac gatcgtaacc cagtgccagg gatcgaggtt tcccacctct
     1021 ccgttgttca gattcaaaaa cgctaccggc tgatcggctt ccaaacggga aacgaggaat
     1081 tcacgaactt gtgcgaacga aggacgattg gctcgatcaa tcggcaccga cagggcggca
     1141 atctcaaagc ccaagtgttt tttgacaaga tattccctca atccccgggt gaagtgggcg
     1201 actgtgtgga cgcccatgat tccgggagtg acaaagctcc aaagctcctc catcagccgg
     1261 atcgaggacg ctttcgtgct gcttttgttt cgttttgcat tatagcggat cagagtgctt
     1321 gcggtcgtcg gtccgcaccc cgctgttcgc tgccaaagct tggagtacca ttcctgatcg
     1381 aagccgtgat agattgtccc gtcatctaca actgtcagct catccaaacg cttcaggaca
     1441 atcactcgat gcatgctctt taccctactt caccaaatca taccgccaaa acatcaaacc
     1501 acccatatcg cgaacgtttg tataccccaa cgctttcagt ttccttctgg cgatgccgct
     1561 gcgggctccg ctctggcaat atacgatgat cggagcatcg agatcactca acaatgcagg
     1621 tttttcagct ccgatgtcat acagggaaag atgaatcgct ccatcgatat gtccgctgta
     1681 atactcaccc tcagtccgga catccaccag gatcacctct tcaccgctct gaagcagtgc
     1741 atagacatcc ttggcggaga ttcgagctcc cccacccact tttttgacga tgaggaagag
     1801 aatcatcaat atgagcgcgt agagtattat tctgttcatt gccgaaaaac tcctgtgtgt
     1861 aaagattgca aaaaccacca ccgacgtcaa cgttcggtgg tggtcgattc actcgattgc
     1921 tttccttagt cggcttcctc cagttgggct tgcaaatatg tttccagcac gtccgccttg
     1981 attgcatcgg ccaaggcttt tcgctcaagc tcctcggctt gaaggcgata cgcctccttg
     2041 aggtttttta ccggatagga agccaacaca tagaccgttc catcatccct gacccatcgg
     2101 tcaacaatat ccactccccg gagggcgata ttcaccgtct ggacggaaag gttttgaagc
     2161 acttccagga tctgtcttgt tctcagggtt tcgcccgctt cctcgatgaa ggtgaccacc
     2221 gaagcatcga tcgaggtttc cgcccatctt ccgatcatat cctttgcagc gctttcggct
     2281 cgaagggcgc tcaacgcctc cgtcgattgc tttgccgaag ctacggcata gtaggtggtt
     2341 cgcgtcctct tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntccgtcg
     2461 attgctttgc cgaagctacg gcatagtagg tggttcgcgt cctcttggga gtgtggttcg
     2521 tccaataagg cgaaccgtcc ttattgaact tgggggtcgt tgcgcatccg gctagaagga
     2581 agaacagaac aagtatgaaa actgtccgtt tcatcatcaa tctcctttca gcagcttggc
     2641 aaacgcctct ttcatcttgg cattcgccgc ttcggcggcc gcgctcggag tgaacgtctc
     2701 aaccaccgct tcggcagccg gctcgaacgc atactggata ttggccagag gaatcgacgc
     2761 cagcacccag acgcccccct ccttgtcaac ccacacgtct tcggtcgtca ccccgaccaa
     2821 tgtcgcctcc gcgacctgct tgctgatcac ttccatcgca tcgagagcct ggcggtcgcc
     2881 gaggcttccg gcatcgttaa ggtaggttac cacgacttcc tgaacgttgg tggagatcca
     2941 gccggcgatc ttgttcctcg cctccgtttc cgcccgcttt atcgaattct ggcggttgct
     3001 catcagccca tacccgcttt caaagtgccg atcacccgcg gtcggggttt tgtataccca
     3061 atccggctgg atgaaccctt cagctccgac aacgaccttg gccgccggaa cggggggtgt
     3121 gacgacggct gcttttttac tcgtggcaca cgaagtacca accatgagga ttgttgccat
     3181 tgcgacaaga aggacgattt ttttcataac gctgtacctc catttttact ggcttaaaag
     3241 cccaatgagt gtttcgattc cgtcgttcag ggcgctttga agcgctttgg tcttggcatc
     3301 cccctgatga agggcggcta tccgctcgct caggctgaag gatcgaacca ccttatcgtt
     3361 ccgcccgagg gtgacgctca gctgatagcg ttcgtaggcg taggggctgg cttcaaagag
     3421 gatcggctcc gaatcatagg tgaccgtcag ggcatagtcg ccgccgcctt gaacaaaccc
     3481 cagattctga agcgcccctt cgaatcccgc tttcaggaaa tgcggatcgg tttcgctgtg
     3541 gacaatgacc tcgaccgaga tcgaccgagc gagggtgcgc agttccgatg cgagggcgcc
     3601 gaggtggtct ggattgatct ctttggtcaa cacccagatt cgatcggcga tctgtcccgc
     3661 ctcctgcgcc accggatacg cggaaagcag gaagcgatag cgctccagtc gatcggcgga
     3721 tgtttgggag cggatcttcg caagctcctt cgtcaattca gccaatcggg tttgaagaaa
     3781 tcgggcgctc tcttggcgct cgaccgcgag acggacgtag gtctggccca tctcgtcggt
     3841 aaaggaggaa acgacctccg atccgacaag atcctcgacg ttcgaagaga ccgtcccctg
     3901 ctcgaagagt gcatcgaagc tcccgccctc gctcgaatcg atcgtggtga tccgggtaac
     3961 ggcttccacc tgggaggaaa acaccgaggc taggttgatc cgggcgtttt caaccgcttt
     4021 ctgataggag gatccgctgc ctacgaccag cagatattta tcctccggat acttcccgtc
     4081 atagggtctg ccgatccagg caggccgctg acttggaatc gacatgcagg aaacaagaaa
     4141 aggaatgaga acaaaaagaa gaatgccttt tctcatcaaa accttcctct agagggaata
     4201 ccgagcctgc tgaatcactt tcttgatcgt ttcctcgccg acccagacct tgcggctcgt
     4261 ttcgatgtcg atcagttcag cggatacgta ataggtacga acccgagtgt ttccttgttg
     4321 atccagaacg ctcctcaccg cgccttggag caggaaatcc gctccgatct cccggccaag
     4381 cgcttttgcg gtctcttcgc tggcgaagta ctgctgctct tcccgctctt ggcggatcga
     4441 agcccggaag ttctgatcgg cgaccatgtc gaccttgccg ctgttgacca gctccatctc
     4501 ataccgcttg gcgatgatcg ccgtgtcgat atgttcgctt gatcgattga tgaacgttcc
     4561 gacgatgacc acaggatttc gtccgaggtt cttcatcctg aaattattga tccatgcacc
     4621 tttcatggag gattggacca gcgcctcgct gacgagacga atatcggtgt cgttccagct
     4681 cccgctgaga tccagatcct tgtcgctgct cactctcgac actgaatagg agctttgaca
     4741 ggcggtaaga aaaacgaggg aaagaagcat gatgatactg ataatgatat ttcggctacg
     4801 gcgcatacga tcctcctgct tctgaaagtg tagcagaaga atggcggatt ggatagaatc
     4861 ctatcattga gaaattgcac ctatcctttg gtaatggtac agttgagtac aaggaggagc
     4921 gtcggtcatc cgaaagatca tacccgtttt gcttgccatc atccttgcaa cctcgtgtgt
     4981 ctcctatgtt gatttgtcat cgactgacgc gtatctgcgc cgcggtcaat acaccaaagc
     5041 gtatgattcg ctgaaaaact cccttgagca tctcgtcgcc gcccagggtc ccctgatcgc
     5101 gagctatgac ctggggatgc ttgcccggct caccggggat tgga
//
LOCUS       DAOSLI010000089         5111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_14572_length_5111_cov_26.375593, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000089 DAOSLI010000000
VERSION     DAOSLI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14572_length_5111_cov_26.375593"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2087
                     /locus_tag="PLF67_08825"
     CDS             <1..2087
                     /locus_tag="PLF67_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOE90152.1"
                     /translation="LRSDEIAILKSNLNHSPNWDNLLVRDPFDPRLIRNSEFYGLIRL
                     GTVSAGAVSFHDFTVPVGIVNSRIVSCDIGDSCSIMECTYLSHYIIDDDVVLYRIGEM
                     QSTNHAKFGNGILKEGEDPEVLITIDVVNEAKGRHIRPFDGMIPADALLWSTYREDRL
                     LMQRFNELTDKVLDSKRGYYGFVGRKTVIKSCDTIKDVWIGEGAYIKGANKLKNLTLR
                     STLAEPVQIGEGVELVNGIIGAGSRIFYGSKAIRFVMGDNSNLKYGARLIHSVLGDNS
                     TVSCCEILNSLIFGSHEQHHNNSFLVAARIEGQSNMAAGANLGSNHNSRNNDGEMIAR
                     RGFWPALSTTVTYDSYFASYTLIAKGSFPHQLSVPLPFSLISSDEISNRRVIMGAYWW
                     MYNLYALERNTHKYHSRDKRKAPRQRYEPAYLAPDTANEIIEARGLLELWVGESLGDG
                     SIDKDSLRAQGKSLLANQAELVGRSTITTYTVENSKEPVIVLKAVESYRAYGEMLLFY
                     AMKTLVAYAANHDQVIDDLLGKRESHLSPWENVGGQLVRRDRVEALLQDLKEGSIKSW
                     DEVHQRYSEWGDSYEEDTLSHALGVLSFLLGEKRPTGDLWKSLVDETKVLVQKIEQEI
                     YHSKEKDHLNHFKYTTYRNEEERDAVLGNLKDDLLIKTVHKEMETFLDQLERVSFLRN
                     SSSASGDRDLEK"
     gene            complement(2018..>3315)
                     /locus_tag="PLF67_08830"
     CDS             complement(2018..>3315)
                     /locus_tag="PLF67_08830"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOE90153.1"
                     /translation="FAYFDEATKLANRNMLIGKLDQTIHDAKESENIAVLHIELENLR
                     IIKDTFGHSIGEQIVIQSATILKQLVGECCCISRLSEGVFVVVLPALEHAEKIEEYIE
                     RLLDSFSHPISTETGIEALFVTLNIGISVYPDNGRDAHDLLKNADLASYQAKRTNKRA
                     VFYTEQLERQIAENAFYTNRLFQSLDNNEFHLEFQPQISCKSGRTVGIEALLRWTIDG
                     DRHVVSDRFIPILEQTGLIYDVGLWVLEEALREHTQLIEKGFQPLRISINLSVVQLQA
                     EHFFEDFLAVIRKSGVAPGYIELEITESFFLENPLDTLEKIYKLKELGVSIAIDDFGK
                     GHSSFNRLKTVPFDRLKIDKEIIDTIDVDKRLAPLTEIIILLARTLGASVTAEGVERK
                     EQADFLKTVDCDEIQGFYFSRSLSPEALEEFLRNETRSS"
     assembly_gap    3316..3415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3416..>5111)
                     /locus_tag="PLF67_08835"
     CDS             complement(<3416..>5111)
                     /locus_tag="PLF67_08835"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOE90154.1"
                     /translation="SISAATAEVSAFWYSFSILGRGFFYSILLHFVMILTKTESLMDR
                     RLMLTLIYIPVWINIILFAPFGYIAEKQYLMTQTDFGWVDVLPVNMGTIWFIAYYSAF
                     SIVSIVILFLWWRRIEPDTPMKRQATCFLLSVLFPFFAGMATDTLPELLGKNAFPKVT
                     IIFMIIPVMTLFIASRRLGLLLEREIPLSLEVNQRQNIDRLRLFQTASAIYTVGAALS
                     LLVGYFGMKKELNNELLLAGFVLVIGLFTRSIPIISKKHAVQNTMFLIANTMGMLFFM
                     TTNAKVGALTIWAIYILFLLFTVILDSNIPAAVFTVLAIAMQIILSFIFPKIQVTIDG
                     SEYATRIFIIVLSFLIVRHITTEYASKIEGYKKIAREQETLEKISSNFISVDIENIRE
                     RIDGMFEMSMAILKFDCMSLMEFSPDYEDTTILNMLRKHGAKPSPYRPGVTVKTDTLP
                     IVSSLIAQKQPIICENTETLPTDEHKEVRDFFISRGVSSFFIFPIMVDNNVGGMLVVE
                     YYDRSNLSSREGRLYFLKMIANILGDTKKKTLYEERLYNFAYFDEATKLANRNMLIG"
BASE COUNT         1164 a         1326 c         1336 g         1185 t
ORIGIN      
        1 atctgagaag cgatgagatc gcaattctca agagcaatct gaaccacagt ccgaattggg
       61 ataatctttt ggtcagagat ccctttgatc cgcgcctgat ccgaaacagt gagttctacg
      121 gcctgattcg gctcgggacg gtctcggcgg gtgcggtgag ttttcacgat ttcaccgttc
      181 ccgtcggcat cgtgaactcc cggatcgtca gctgcgatat cggggattcc tgctcgatca
      241 tggagtgcac ctacttaagc cactacatca tcgatgatga cgtcgtgctc taccggatcg
      301 gggagatgca gagcaccaac cacgcgaagt tcggcaacgg gattctgaag gagggggagg
      361 atcccgaggt cctgatcacc atcgatgtcg tcaacgaggc gaagggacgg catatccggc
      421 cctttgacgg gatgattccc gccgatgcgc tcctctggtc gacctaccgg gaagaccggc
      481 tgctcatgca gcggttcaac gagcttaccg acaaggtcct cgattccaag cggggctact
      541 acggcttcgt cggcaggaag accgtgatca aatcgtgcga cacgatcaag gatgtatgga
      601 tcggcgaagg ggcgtatatc aaaggagcga acaagctgaa gaacctgacc ctccgatcga
      661 ccctggccga gccggttcag atcggcgagg gagtcgaact ggtcaacgga atcatcggcg
      721 cgggctcccg gatcttctac ggctccaaag cgatccgctt cgtgatgggg gacaactcca
      781 acctcaaata cggcgcccgc ttgatccact ccgtgttggg ggacaactcc acggtcagct
      841 gctgcgagat cctcaacagc ctgatcttcg gttcgcacga acagcaccac aacaactcct
      901 tcctcgtcgc cgcccggatc gaaggacaat ccaacatggc cgccggggcg aacctcgggt
      961 cgaaccacaa cagccggaac aacgacgggg agatgatcgc ccgccggggg ttctggccgg
     1021 cgctctccac caccgttacg tacgattcat atttcgccag ctataccctg atcgccaaag
     1081 gctcctttcc ccaccagctt tccgttcccc tgccgttcag cctgatttcc agtgacgaga
     1141 tcagcaaccg ccgggtgatc atgggagcgt actggtggat gtacaacctc tatgcattgg
     1201 agcggaacac ccataaatac cacagccgcg acaagcgcaa ggctccaaga cagcgctatg
     1261 agcccgccta ccttgccccc gatacggcga atgagatcat cgaggcgcga ggcttgcttg
     1321 aactttgggt aggcgagagc ttgggagacg gaagcatcga taaggattcc cttcgtgctc
     1381 aaggcaagtc cttgcttgcc aatcaagccg agttggtggg gcgttcaacg atcaccactt
     1441 atacggtgga gaactcgaag gaaccggtga tcgtcctcaa agccgtcgaa tcctaccggg
     1501 cctatggcga gatgctcctt ttctacgcga tgaagacctt ggttgcctat gccgccaacc
     1561 acgatcaggt gattgacgat cttcttggga agagagaatc tcacctgagt ccttgggaaa
     1621 acgtcggtgg acagcttgtt cggcgcgacc gggtggaagc gctccttcaa gacctcaaag
     1681 aaggatcgat caagtcgtgg gatgaggttc atcagcggta ttccgagtgg ggggactcct
     1741 atgaagagga caccttgtcc catgccctcg gggtgctctc cttcctcctt ggggagaaga
     1801 ggccgaccgg tgatctgtgg aagagtctcg ttgatgaaac caaagttctt gttcaaaaga
     1861 ttgagcagga gatctaccac tccaaggaga aggatcatct caatcacttc aagtacacca
     1921 cctaccgtaa tgaagaagag cgcgacgcgg tcttggggaa tctgaaggat gatctgctga
     1981 ttaagaccgt ccataaggag atggagacct tcctcgatca gctggaacgc gtctcgtttc
     2041 tgaggaactc ctccagcgct tccggcgaca gggatcttga gaagtagaac ccttggatct
     2101 cgtcgcagtc gacggttttc aggaaatccg cctgctcctt ccgttccacg ccttcggccg
     2161 taacgcttgc ccccaaggtt cgggccagca ggatgatgat ctccgtcagg ggagccagac
     2221 gtttgtccac atcgatcgta tcgatgatct ccttgtcgat cttcaaccgg tcgaacggga
     2281 cggttttcaa ccggttgaac gatgaatgtc ccttgccgaa atcgtcgatg gcgatgctga
     2341 cacccagttc tttcagcttg tatatcttct caagggtgtc cagcggattt tccagaaaga
     2401 agctttccgt tatctccagc tcaatatagc cgggcgccac cccgcttttt ctgatgaccg
     2461 caaggaaatc ctcgaagaag tgttccgcct gaagctgaac gacggacagg tttatcgaaa
     2521 tgcgaagagg ctgaaatccc ttttcaatca gctgggtgtg ctcccgaaga gcctcttcca
     2581 gcacccagag ccccacatca tagatcaatc ccgtttgttc gaggatgggg atgaatcgat
     2641 ccgataccac gtgtctgtcc ccatcgatcg tccatcggag taacgcctca attccgacgg
     2701 ttcttcccga cttgcagctg atctggggct gaaattccag atggaactca ttgttgtcca
     2761 acgattggaa gagccgattc gtatagaagg cgttttccgc gatctgccgt tccagctgtt
     2821 cggtatagaa gacggccctc ttgttcgttc ttttcgcttg atagcttgcc aaatcggcgt
     2881 tcttcaacag gtcgtgcgca tctcttccat tgtccggata cacggagatg ccgatattga
     2941 gcgtaacgaa gagcgcttct attcccgtct cggtcgatat gggatgggaa aaagaatcaa
     3001 gcagccgctc tatatactcc tcgatctttt cggcatgttc cagtgcaggc agaacgacga
     3061 cgaacacccc ttcactcaat cgtgagatgc agcagcattc ccccaccagc tgcttaagaa
     3121 tcgtcgccga ctgtattact atctgttccc ctatgctatg gccgaaggtg tctttgatga
     3181 ttcgcaagtt ctccagctcg atatgcagaa ccgctatatt ctccgattct tttgcgtcat
     3241 ggatggtttg gtcaagtttt cctatcagca tattcctgtt cgccagtttt gtggcttcat
     3301 cgaaatacgc gaagtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctat
     3421 cagcatattc ctgttcgcca gttttgtggc ttcatcgaaa tacgcgaagt tgtacagtct
     3481 ttcttcgtag agggtctttt tcttcgtgtc gcccaggatg ttggcgatca ttttcaagaa
     3541 gtacagccgg ccttcccgtg agctcagatt gctccggtcg tagtactcga cgacgagcat
     3601 cccgccgacg ttgttgtcga ccatgatcgg aaagatgaag aaggagctca cccctcttga
     3661 tatgaagaaa tctcttacct ctttatgctc atcggtggga agagtctcgg tgttctcaca
     3721 gatgatgggc tgtttctgag cgatcagcga actgacgatc ggcaacgtgt cggttttgac
     3781 ggtcactccc ggtcgatagg gagatggttt ggcgccatgt ttcctaagca tattgagaat
     3841 cgtcgtatct tcatagtccg ggctgaattc catcagactc atgcaatcga acttcaggat
     3901 ggccatcgac atttcgaaca ttccgtcgat ccgttcccgg atattttcga tatcgaccga
     3961 gatgaagttc gaggagatct tttcaagtgt ctcctgttct cttgcgatct tcttataccc
     4021 ctcgatcttt gaagcatatt cagtcgtgat atgccgcacg atgagaaagg ataggacgat
     4081 gatgaaaatt cttgtcgcat attcgcttcc gtcgatggtg acttgtattt tcgggaaaat
     4141 gaacgagagg attatttgca tcgctatcgc caatacggta aacacggcgg cggggatgtt
     4201 gctatcaagg atgacggtga agagaagaaa caggatgtag atcgcccaga tcgtcagtgc
     4261 tcccaccttg gcgttcgttg tcatgaaaaa cagcatgccc atcgtgttcg ctatgaggaa
     4321 catcgtgttc tggacggcgt gtttttttga gatgatcggt atcgatctcg tgaagagccc
     4381 gatcaccaag acgaacccgg cgagaagcag ctcgttgttc aactccttct tcatgccgaa
     4441 atatccaacc agcagggaca gcgctgctcc caccgtgtag atcgccgatg ccgtttggaa
     4501 caaacgcagc cggtcgatgt tctggcgctg gttgacttcc agagacaagg ggatctcccg
     4561 ttccagaagg agcccgagcc tccttgatgc gatgaagagc gtcatcacgg gaatgatcat
     4621 gaagataatc gtcactttgg gaaaagcgtt ttttccgagg agctcgggca gggtatccgt
     4681 cgccattccc gcgaaaaagg gaaagaggac ggaaagcagg aagcatgtcg cctgccgctt
     4741 catgggggta tcaggttcga tgcgcctcca ccagagaaag aggattacga tacttacaat
     4801 cgaaaatgcc gagtagtagg cgatgaacca gattgttccc atattgacgg gaaggacgtc
     4861 tacccagccg aaatcggttt gcgtcatcag gtattgtttc tctgcaatat atccgaaggg
     4921 cgcaaagaga atgatattga tccagacggg gatgtagatc agcgtcagca tgagccgcct
     4981 atccatcagg ctttcggttt ttgtcaggat catcacgaag tggagcagga tgctgtagaa
     5041 aaaacccctt ccgagaatcg aaaaggaata ccagaatgca cttacctccg ccgtggccgc
     5101 cgagatcgag a
//
LOCUS       DAOSLI010000090         4983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_15056_length_4983_cov_33.280438, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000090 DAOSLI010000000
VERSION     DAOSLI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15056_length_4983_cov_33.280438"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLF67_08840"
     CDS             <1..447
                     /locus_tag="PLF67_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOE90155.1"
                     /translation="ESYKAYGVTNHRHTVEIEQELSILNKEPLLVQFTPHLVPMNRGI
                     LSTCYADLKEGVTEKEVAEAYEKWYKDEAFIRLMGSNLPETRYVHSTNTVAIGWKVDP
                     RTKRVVALGAIDNLVKGAGGQAVQNMNIRFGLDEATGLKKWIANPL"
     gene            459..1682
                     /gene="argJ"
                     /locus_tag="PLF67_08845"
     CDS             459..1682
                     /gene="argJ"
                     /locus_tag="PLF67_08845"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HOE90156.1"
                     /translation="MYTIMEGGVTAPKGYQAGGIACGIKKRKKDLALIYSETPCTFAG
                     SFTTNLVKAAPVLWDQKVIETSGTVHCILVNSGNANAATGEKGLRDAQASAALVADRF
                     GIHPEQTLVCSTGVIGLPLPMETIEKGIASIVQTLDTTNEAALNAAQAILTTDTFTKQ
                     VAVEVLVDQKPVRIGGMAKGSGMIHPNMATMLSFITTDAKISKADLQALLGSSIEDTY
                     NMISVDGDTSTNDTVLVLANGEAENEMLSPSHPDWKAFVKAFDHVNAELAKLIVKDGE
                     GAQKFIEMTVRGAKDKETSRTLARSVVSSNLVKTAFFGSDANWGRIFCAMGYSGARFN
                     PQKTDLFFASEKGKIQVLKDGEPLPFDEREAKRILSEPVIYCTAVLKDGEASATAWGC
                     DLSYEYVRINGDYRS"
     gene            1679..2569
                     /gene="argB"
                     /locus_tag="PLF67_08850"
     CDS             1679..2569
                     /gene="argB"
                     /locus_tag="PLF67_08850"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOE90157.1"
                     /translation="MTTHTVAQEILKAETLIEAIPYIRRFAGAIAVVKYGGSAMVNAE
                     LKKAVIEDIAMLKYIGLKPVVVHGGGAEISSLLARLGKESVFIDGLRVTDEETASVAE
                     MVLSGSIGKSLVDNLEQVGIKAVGISGKDGRTFLCEKKKDEKGRDLGFVGEIKKVDTT
                     LVDSLLKSNFVPVLSPVGVDTEGNTYNINADYAASAIAGALSAQKLIFLTDVEGILKD
                     KDDPSSIIRTLTHGEAVSYIEQGIIKGGMIPKVQCCLDGLEKGVESVHVLDGRLPHAI
                     LLEIFTTRGIGTMVTKEKTP"
     gene            2566..3735
                     /locus_tag="PLF67_08855"
     CDS             2566..3735
                     /locus_tag="PLF67_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOE90158.1"
                     /translation="MSNNHLMNTYSQWPVTFVGGEGAYLKDDKGDAYLDFVGGIAVNA
                     LGYKHEGLAAAIREVLDSGVLHVSNLYYNAHVLEAAKKLAALSGMEKVFFCNSGAEAN
                     EAALKLARKWGSGKNKSTIISMLDSFHGRTYAAVTATGQKKYHQHFAPLPPGFVYAHF
                     NDLDSVKALVDERTCAVIVEPIQGEGGIVPATKEFLHGLRDLCDTHELLLIYDEVQCG
                     MGRSGKPFAHQLYGVKPDILTTAKALAGGVPAGAMLTQGEASDVFVPGDHASTFGGNA
                     LAARAIIYMCDTLQEKAFLSHVEAMGNYLRMKLEELKVQFSDLITAVRGVGLINGIAL
                     AIPPKEVVAKCFEKKLLLASAGSNVVRFVPPLIITEKEIDAAVKILQESLSEVRS"
     gene            complement(3730..4332)
                     /locus_tag="PLF67_08860"
     CDS             complement(3730..4332)
                     /locus_tag="PLF67_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE90159.1"
                     /translation="MTEERPLSKSQRTKAKIFEMAWNLFKGRGFDNVSLQDIADHSGT
                     AKGSFYTYFSTKSDIIVEGFWMIDGYYQTVEKEVLRHQTTEEKLLKFTELQLTYVRDE
                     IGVEMLKVLYANQVLQQGSAKVITNPTRFWHTFIRSIIEKGQINGECRTGLSAEQYAL
                     FFNRSMRGLFLDWNITSASFDLVEEGLLYCKKFVLPTILL"
     gene            complement(4342..>4983)
                     /locus_tag="PLF67_08865"
     CDS             complement(4342..>4983)
                     /locus_tag="PLF67_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOE90160.1"
                     /translation="FMDYLSTRISCDYLNESLPIEGVGALLLIEIDGIDEAVVEQQLI
                     QVGDLCMEQGAIEVYIAEDANNRERVWSVRRAIAEAIKVYSPVQSLEDIVVPIGSIPK
                     VIPFLDKLSKEHDVLIPCYGHAGDGNLHATVIKNPEFTMEHWLEVEPKILGELYEFIT
                     GELGGKISGEHGIGLKRREFFKEFTSPIEYRVMEQIKRALDPNGIMNPGKILL"
BASE COUNT         1133 a         1362 c         1470 g         1018 t
ORIGIN      
        1 gaatcgtaca aagcgtacgg ggtgacgaac caccgccaca cggtggagat cgagcaggag
       61 ctgagcattt tgaataaaga accgctgctt gtccagttca ccccgcacct cgttccgatg
      121 aaccggggga ttctaagcac ctgctacgcc gacctcaagg agggggtgac ggagaaagag
      181 gtcgccgagg cgtatgagaa gtggtacaag gatgaggctt tcatccgctt gatgggttca
      241 aacctgccgg aaacccggta cgtccactcg accaatacgg tcgccatcgg gtggaaagtc
      301 gatccccgga caaagcgggt ggtcgccctc ggggcgatcg acaacctggt caaaggcgcc
      361 ggcggccaag cggtgcagaa catgaatatc cgattcgggc tcgacgaggc gacgggtctt
      421 aagaaatgga tcgccaaccc cctgtaggag aagatgggat gtatacaatc atggaaggcg
      481 gagtcaccgc gccgaaaggg tatcaggcag gcgggatcgc ctgcggcatc aagaaacgaa
      541 agaaagatct tgccctgatc tattccgaga ctccctgcac ctttgccggc agcttcacca
      601 ccaatctcgt gaaagccgct ccggtgctct gggatcaaaa ggtgattgaa acaagcggga
      661 cggtccactg cattctcgtc aacagcggca acgccaatgc ggcgaccggt gaaaaaggat
      721 tgcgcgatgc acaagcgagc gccgccctcg tcgccgaccg attcggcatc cacccggagc
      781 agacgctggt gtgctccacc ggagtgatcg gcttaccgct tccgatggaa acgatcgaaa
      841 aagggatcgc ttcgatcgtc caaacccttg ataccaccaa cgaagcggcc ctgaacgccg
      901 ctcaggcgat tctcaccacc gacacgttca cgaagcaggt ggcggttgaa gtgcttgttg
      961 accaaaagcc ggtccgcatc ggcggcatgg cgaaagggag cgggatgatc catccgaaca
     1021 tggccacgat gctctccttc atcaccaccg atgccaagat ctccaaagcg gatcttcaag
     1081 cgctcctcgg ttcttcaatc gaggatacct acaatatgat cagtgtcgac ggggatacct
     1141 cgaccaacga cacggttctg gtcctcgcca acggcgaagc ggaaaatgag atgctttctc
     1201 cttcccatcc cgactggaag gcgttcgtca aggcgttcga ccatgtgaac gccgagctgg
     1261 cgaaactgat cgtcaaggac ggagaagggg cgcaaaagtt catcgagatg accgtcagag
     1321 gcgctaagga caaagagacc tcccggactc ttgcccgcag tgtggtctcc tccaatctgg
     1381 tgaagacggc gttcttcggc agcgatgcga actgggggcg gatcttctgc gccatggggt
     1441 acagcggggc ccgattcaac ccgcagaaga cggatctctt cttcgcttcg gaaaagggca
     1501 agattcaagt cctcaaagac ggggagccgc tgccctttga tgaacgggag gcgaagcgga
     1561 tcctctcgga accggtgatt tactgcaccg cggttctcaa ggacggggaa gcctccgcca
     1621 cggcgtgggg gtgcgacctt tcctacgagt acgtccgaat taatggagat tatcgaagtt
     1681 gacgacacat acagtagccc aggaaatttt gaaagcggaa accctcatcg aggcgatacc
     1741 gtacattcgg cggttcgccg gagcgatcgc cgtcgtgaag tatggcggtt cggcgatggt
     1801 caatgccgag ctgaaaaaag cggtcatcga agatatcgcg atgctgaagt atatcggtct
     1861 gaaaccggta gtcgtccacg gcggcggagc ggagatctca agcctccttg cccgtctcgg
     1921 caaggagagt gtcttcatcg acgggctgag ggtgacggat gaggagaccg cttcggtggc
     1981 cgagatggtg ctttccggct cgatcgggaa atccctggtg gacaacttgg agcaggtcgg
     2041 catcaaggcg gtggggatca gcggcaagga cggccggacg ttcctgtgcg aaaagaaaaa
     2101 ggatgagaaa ggccgggacc tcggcttcgt cggagagatc aaaaaggtcg acaccacctt
     2161 ggtggattcc ctcttgaaga gcaacttcgt ccctgtcctt tccccggtcg gggtcgacac
     2221 cgagggaaat acctacaata tcaacgccga ttatgccgcc agtgcgatcg ccggagcgct
     2281 gtcggcccaa aagctgatct tcctgaccga tgtggagggg attctcaagg acaaggacga
     2341 tccttcctca atcatccgaa ccctgaccca cggcgaggcg gtttcctata tcgagcaggg
     2401 gatcatcaaa ggcgggatga ttcccaaggt tcaatgctgc ttggacgggc tggaaaaagg
     2461 ggtagagagc gtccacgtcc tcgacgggcg actgccccat gcgattctcc ttgagatctt
     2521 cactacgcgg ggaatcggaa cgatggtcac caaggagaaa acaccatgag caacaaccat
     2581 ctgatgaata cctattccca atggcccgtc acgttcgtcg gcggcgaagg ggcgtatctg
     2641 aaggatgaca agggtgatgc gtatctcgat ttcgtcggag ggatcgcggt caacgcgttg
     2701 ggatataagc atgaggggct tgccgccgcc atccgggaag ttctggattc gggagtgctc
     2761 cacgtttcca acctctacta caacgcccat gtgctggagg cggcaaagaa gcttgccgcc
     2821 ctgagcggga tggagaaggt attcttctgc aacagcggag cggaagcgaa tgaagcggcg
     2881 ttgaagcttg cccggaagtg ggggagcgga aagaacaaat ccacgatcat ctcgatgctt
     2941 gattcctttc atggccggac gtatgcggcg gtgacggcga ccgggcagaa aaaataccac
     3001 cagcacttcg ctccgcttcc ccccggattc gtctatgcgc atttcaacga ccttgattcg
     3061 gtgaaggcgc tcgtcgatga acggacgtgc gcggtcatcg tcgagccgat ccagggcgag
     3121 ggcggaatcg tcccggcgac aaaggagttc cttcacggcc tgcgcgatct gtgtgacacc
     3181 cacgaactgc tactcatcta cgatgaggtg caatgcggaa tggggcgaag cggaaagccc
     3241 ttcgcccatc agctctacgg ggtgaagccg gatattctca ccaccgccaa agcgcttgcc
     3301 ggaggggttc cggcgggggc gatgctgacc caaggggaag caagcgatgt cttcgttccc
     3361 ggggaccacg cttccacatt cggcgggaac gccttggcgg cgcgggcgat catctacatg
     3421 tgcgatactc ttcaagagaa agcatttctt tcccacgttg aagcgatggg aaactacctt
     3481 cgaatgaagc tcgaggagct caaagttcaa ttttccgatc tcatcaccgc cgtccgcggg
     3541 gtgggcctga tcaacggcat cgcgcttgcg atccctccga aagaggtggt ggcaaagtgc
     3601 tttgagaaga agctccttct tgcaagcgcc ggttccaacg tcgttcgctt cgttccgccg
     3661 ctcatcatca ccgagaagga gatcgatgcc gccgtcaaga ttcttcaaga aagcttgagt
     3721 gaggtacgtt catagcagga tcgtcggcag gacgaacttt ttgcaataga gaagaccttc
     3781 ttcaacgaga tcgaaggagg ccgaggtgat gttccaatca aggaagaggc cgcgcattga
     3841 gcggttgaag aacagggcat actgttcagc cgagaggccg gttcgacact ctccgttgat
     3901 ttggcccttt tcaatgatcg agcggatgaa cgtatgccag aaccgggtcg gattggtgat
     3961 gaccttcgcc gagccttgct ggagcacctg gttggcgtac aggaccttca gcatttcgac
     4021 cccgatttca tcgcggacat aggttagctg gagctcggtg aacttcaaaa gcttctcttc
     4081 ggttgtttga tgacgcagga cctctttctc caccgtttga tagtacccgt cgatcatcca
     4141 aaacccttcg acgatgatgt cgctctttgt cgaaaagtag gtgtagaagc ttcccttggc
     4201 cgtgccggaa tgatcggcga tatcctgaag ggatacgttg tcgaatcccc gtcccttgaa
     4261 gaggttccat gccatttcga agatcttcgc cttggtgcgt tgggattttg acaatggtcg
     4321 ttcctcggtc acaccatgct cctacagaag gatcttcccc gggttcatga tcccgttcgg
     4381 gtcaagggct cgtttaatct gctccatcac ccgatactcg atcggagaag tgaactcctt
     4441 gaagaactcc cttcgcttga ggccgatccc gtgctcgccg ctgatcttcc caccgagttc
     4501 accggtgatg aactcataca gttcgccaag gattttaggt tcgacctcaa gccaatgctc
     4561 catcgtgaat tcggggttct tgatcaccgt ggcgtggagg ttcccgtctc cggcatgccc
     4621 gtaacagggg atcaggacgt catgttcttt ggagagctta tccaaaaagg gtatcacctt
     4681 ggggattgag ccgatcggaa cgacgatgtc ttcaaggctt tggaccgggc tgtacacctt
     4741 gatcgcctcg gcgatcgccc gccggaccga ccagacccgt tcccggttgt tcgcatcttc
     4801 ggcgatatat acctcgatcg ctccctgctc catgcacaga tcgcccactt ggatgagctg
     4861 ttgttcgacg accgcttcat cgatgccgtc gatctcgatg aggagcaacg ctccgactcc
     4921 ctcgatcgga aggctttcat tgaggtagtc acaggagatt ctggtggaga ggtagtccat
     4981 gaa
//
LOCUS       DAOSLI010000091         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_15448_length_4883_cov_12.052610, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000091 DAOSLI010000000
VERSION     DAOSLI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15448_length_4883_cov_12.052610"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PLF67_08870"
     CDS             <1..935
                     /locus_tag="PLF67_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE90161.1"
                     /translation="SRVLNGTKFVSEELQRKVLEAIERQGYVANHVARSMVLKKTFTV
                     GLIMPVVSELYHQMIFTHAEFILEEAGYKVLVCRVKDTAQSVQSYLDLLLENRVDGMI
                     LMHETSNPKVLEQLKKSQIPIVLTGIDIPGLSFPQVRIDDYQAAYDGTKYLLAHRRKR
                     IALIGGWGYSVGDKRLEGYKTALAEAGIEFDSSLIIPGLYTMQSGSQAVNYLIRERIE
                     FDAVFALSDEMAVGVMKELLEQGKGIPSDVAVLGFDGIELGRYTNPTLATVKQPIEQI
                     GELAAERLLAMLNGEESEDSLILPHSISSGSSVG"
     gene            1034..2305
                     /locus_tag="PLF67_08875"
     CDS             1034..2305
                     /locus_tag="PLF67_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE90162.1"
                     /translation="MKKMILFVLTFLLAIGLVTAAGAKEVGSKTSITLWDQYYRGVES
                     DIMDSLVKDFEAKNADIQIVRETKTLDDLKLVLKMSLESGTGPDIMQVNQGEADMGAM
                     AKAGLLVKANDAAVKNNWAARFSDSTLKAMGYGGDYYGIATTAEVVGFFYNKPLMQKL
                     GLSVPKTFTELETLLERTKRAGYTPINFGNLDGWTGIHEWSAIQHVLTTREQLDAMMS
                     GKPGNYWNSVENRQAAEILQRWVRSGYFTKDFSAIGYDDSASIFMQGDSLLFLTGNWM
                     MGELLENSPFEVGFFLVPANDPSRLKAVGGPGIPFVVSQKSTKADAAMKFINYLATEE
                     AAKAWADNMMLPAQSIDANEYRDAPLLFREILDAYSTADKANGMGYFIDWITPTFYDT
                     CSAAVQELMALEITPEVFVQKLQNDYASFTN"
     gene            2394..3284
                     /locus_tag="PLF67_08880"
     CDS             2394..3284
                     /locus_tag="PLF67_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE90163.1"
                     /translation="MKSKRSVYDSPWRGLLYLMPGLITYALFILIPVVQTILLGMMDW
                     SGLGKKTFIGFQNYLELFSDPIFFQSLANNAVLVFYIMILPTLLGLVLASIIELNDIA
                     FRKLFEVMFFMPYILSLVVVGVIWRWIYNPAFGVLNTVLSAVGLSSLSRAWLGESQTA
                     LAAVGFTGTWVHYGFAMVVFLAGYSRISPSLYDAVALDGGGKWHEFRHVALPSLTNEM
                     VVVGVYLFINSLKTFDLVYVMTKGGPGYSTNVLSLYVFRNAFQYNRNGYAAALAVTLM
                     VIIFGITLLVTRARRRYEQA"
     gene            3271..4110
                     /locus_tag="PLF67_08885"
     CDS             3271..4110
                     /locus_tag="PLF67_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE90164.1"
                     /translation="MSRHRVAFSFSRIITIAVLLLFLVISVAPFLLVWSAAFKTKADL
                     VTNVLGPPKTFHFENIRKAWIQGRFGTYYKNSILVVVPVVAGTILLSLANAYAFAYFR
                     IPAKELLFGIILLGMTIPMEVVIIQLYYHLLNLGLLNTLKGLVLAQIGMGIPFGTFFL
                     RGTLKEIPRSLIDAAEIDGAGTFAILTRILIPMSGSAIMTLIVFFFTWTWNEFLLTLV
                     VIHSDSLRTLPVGMAFFQGKYTGDTPLIAMGAALMTLPIIILYIVLQKYVISGITAGA
                     LKE"
     gene            4196..>4883
                     /locus_tag="PLF67_08890"
     CDS             4196..>4883
                     /locus_tag="PLF67_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOE90165.1"
                     /translation="MKLTILGAASPRFPLLLNSLLKRENLSIDRVCLFDLDREKLGLL
                     SRTLLPEIMTHATGRFDLSAAATIEEAFEGADYVFSSIRVGGQHRRALDETHAVELGE
                     IGQETVGIGGFLSALRTIPVMLEHVDFMQRYAPEATLINFTNPSGIVTQAVHSLSGYS
                     KIVGICDAPQMVATYTAEIYEVDPSEVQILSYGLNHYGWVYSVQVRGIEVLDQLLDCR
                     REEFFAKEPFY"
BASE COUNT         1094 a         1262 c         1412 g         1115 t
ORIGIN      
        1 tgtcacgggt gttgaacggg accaaattcg tgagcgaaga gcttcagagg aaagtccttg
       61 aggcgattga gcgtcaggga tatgtcgcca accatgtggc tcggagcatg gttctcaaga
      121 agacgttcac cgtgggcctg atcatgcccg tcgtgtccga gctctaccac cagatgatct
      181 tcacccacgc cgaattcatc cttgaggagg ccggctacaa ggtgctggtc tgccgggtga
      241 aagacactgc ccaaagcgtc cagtcctact tggacctcct gctggagaat agggtggatg
      301 gaatgatcct gatgcatgag accagcaacc ccaaggtgct cgagcagctc aagaaaagcc
      361 agataccgat cgtccttacc ggaatcgaca tccccggcct ttcctttccg caggtacgga
      421 tcgatgacta tcaggctgcc tatgacggaa caaagtacct gcttgcccat cggaggaaac
      481 ggatcgccct gatcggagga tgggggtatt ccgtcgggga caagcggctc gaagggtata
      541 agaccgcact ggcggaagcc ggcatcgagt ttgactcctc gctcatcata ccgggactct
      601 acacgatgca atcgggatct caggcggtga attacctgat cagggaacgg atcgagttcg
      661 atgccgtgtt cgccttgagc gacgaaatgg cggtcggggt gatgaaagag ttgttggagc
      721 aggggaaggg gattccttcg gacgtcgccg tcctggggtt tgacgggatt gagctgggcc
      781 gatacaccaa tcccacattg gcgaccgtca agcagccgat agaacagata ggagaattgg
      841 ccgccgagcg gctgctggcg atgctcaacg gggaggagag cgaagattcg ctcattcttc
      901 cgcacagcat ctcttccggc agcagcgttg gatgatcggt tgagaggatt cggtcaaata
      961 cgcaaagagg aaagaaataa tttgacagga aatcgttttc cttgtaccct caaagtaaag
     1021 aggaggaaac agcatgaaaa aaatgattct ctttgtgctg acttttttac tggcaatcgg
     1081 gttggtcacc gcagccggtg cgaaggaggt cggatccaaa acatccatca ccctctggga
     1141 tcaatattac cggggggttg agagtgacat catggattcg ctggtcaagg actttgaagc
     1201 gaagaatgcc gatattcaga tcgtccgtga aacgaaaacc ctggatgatt tgaaattggt
     1261 gctgaaaatg tcgcttgagt ccggaacggg gccggatatc atgcaggtga accaaggcga
     1321 agccgacatg ggcgccatgg cgaaggcagg acttctggtc aaggcaaacg atgccgcagt
     1381 gaaaaacaac tgggcggccc gtttttccga ttccacgctg aaagcgatgg gatatggcgg
     1441 agactactac ggcatcgcca ccacggcgga ggtcgtcggc ttcttctaca acaagccgtt
     1501 gatgcagaag ctgggcctct cggttcccaa gacgttcacc gagctggaaa ccctgcttga
     1561 acggacgaaa cgggcggggt acaccccgat caatttcgga aacctggatg gctggaccgg
     1621 aatccatgag tggagtgcga tccagcacgt tcttacgacc agagagcagc ttgatgcgat
     1681 gatgagcgga aaacccggga attactggaa cagcgtggag aacaggcaag ccgccgaaat
     1741 tctccagcgc tgggttcgca gcggctattt cacaaaagat ttctcggcga tcgggtatga
     1801 cgattcggca agcatcttca tgcaaggcga ttcattgctc tttttgacgg gaaactggat
     1861 gatgggcgaa ctgttggaaa actcgccgtt tgaggtcgga ttcttccttg ttcccgccaa
     1921 cgatccttcc cggctgaaag cggtcggagg acccggcatc ccgttcgtcg tatcgcagaa
     1981 atcaacgaag gccgatgcgg cgatgaagtt cataaactat ctggcgaccg aggaagcagc
     2041 aaaagcatgg gcggacaaca tgatgcttcc ggcacaatcg atcgatgcca acgagtatcg
     2101 tgacgctccg ctgctgttcc gggagatcct tgatgcgtac agcaccgcgg acaaggcgaa
     2161 tggaatggga tatttcatag actggattac gccgacgttc tatgacacct gctcagccgc
     2221 cgtccaggag ctgatggccc ttgagatcac accggaagtg ttcgtgcaga aactgcaaaa
     2281 cgattacgca agtttcacaa actgacgagt gtgctgcgaa gaagaccatc acccttctcc
     2341 gtttgagggg ggtgatggcc ttgtaatctg agaaaacggg gaggggtgtc tacgtgaaat
     2401 caaagcgcag tgtatatgac tcgccgtggc gaggtctctt gtacctcatg ccgggcctca
     2461 tcacgtacgc cctctttatc ttgattccgg tcgtccagac gattcttctt ggaatgatgg
     2521 actggagcgg attgggaaaa aagacattca tcggattcca aaactatctg gagctgtttt
     2581 ccgatccgat cttcttccag tcattggcga acaacgccgt cctcgtcttc tacatcatga
     2641 tcctgccgac ccttctgggg ttggttctcg ccagcatcat cgagctgaac gacatcgcct
     2701 tccgcaaact cttcgaagtc atgttcttca tgccctatat tctttccctc gtcgtcgtcg
     2761 gagtgatctg gcgctggatc tacaaccccg ctttcggggt attgaatacc gtgttgtcgg
     2821 ctgtcgggct ctcttctctg agccgggcat ggctgggtga aagccagacg gcgcttgcgg
     2881 cggtcggctt taccggcaca tgggtgcact acggattcgc gatggtcgtc tttctcgccg
     2941 gatacagcag aatttcaccg agtctgtacg atgccgtcgc ccttgacggg ggtggcaagt
     3001 ggcatgagtt ccggcatgtc gccctgccgt ctctgacgaa tgaaatggtg gtcgtcggtg
     3061 tatatctgtt catcaactcg ctcaagacct tcgacctcgt ctatgtgatg acgaaagggg
     3121 ggccggggta ctcaacgaac gtcctttccc tctatgtgtt caggaatgcg ttccagtaca
     3181 accggaacgg gtatgcagcc gcgctggcgg taacgttgat ggtcatcatc ttcgggatta
     3241 ctcttttagt aacccgggca aggaggcgct atgagcaggc atagagtcgc tttctcattc
     3301 agccggatca tcacgatcgc cgttctgctc ctcttcctgg tcatttcggt cgcgccattc
     3361 ctgttggtat ggagcgctgc attcaagacc aaggcggatc tggtcaccaa cgtgctcgga
     3421 ccgccgaaaa cgtttcactt cgaaaatatc aggaaagctt ggatccaggg ccgattcggg
     3481 acgtactaca agaacagcat ccttgtggtt gtccccgtgg ttgcgggaac gatcctcctt
     3541 tcgcttgcca atgcctacgc attcgcatat ttcaggattc cggcgaaaga gcttctgttt
     3601 ggaatcatcc ttctgggaat gacgatcccg atggaggtcg tgatcatcca gttgtactat
     3661 catctgctga acttgggact gctcaacacg cttaaggggc tcgtgttggc ccagatcgga
     3721 atgggcatcc ccttcgggac gttcttttta cggggaacgt tgaaagaaat cccccgttcg
     3781 ctgattgatg cggcggagat cgacggggcg ggcacctttg cgatattgac ccggattctt
     3841 attccgatgt ccggttcggc gatcatgacc ctgatcgtgt tcttcttcac ctggacgtgg
     3901 aatgaattcc tgctcactct ggtggtcatc cacagcgatt cactgaggac gctgccggtg
     3961 ggcatggcgt tcttccaagg taaatatacc ggggatacgc ccctcatcgc gatgggcgcg
     4021 gcgctgatga ccctgccgat catcatcctg tacatcgtat tgcaaaagta cgtgatctcc
     4081 ggaatcaccg ccggagcatt gaaggagtag gaggggacat gatggaaaga tgcgaagtgt
     4141 tccttgatgg gaaaacgctt acacagggag gtgagcgcat gcaaggagga aagccatgaa
     4201 gctcacgatt ctcggcgcgg cgagcccccg ctttcctttg ctgctcaatt ccctgctcaa
     4261 acgggagaat ctttcaatcg accgggtgtg tctcttcgac ctcgaccggg agaagcttgg
     4321 cctgctgtca agaacgcttc tgcctgagat catgacccat gcaaccggac gattcgacct
     4381 ttctgccgcc gccacgatcg aggaggcgtt cgaaggagcc gactacgtct tctcttcgat
     4441 ccgggtcggt gggcagcacc gacgggcgct cgatgaaacg catgcggtcg aacttggcga
     4501 gatcgggcag gagaccgtcg gaataggcgg gtttctgagc gccctcagga cgattccggt
     4561 catgctggag catgtggatt tcatgcagcg atacgcccct gaagccaccc tgatcaactt
     4621 tacgaatcca tcgggcatcg tcactcaggc ggttcactcg ctttccggat attccaagat
     4681 cgtgggaatc tgcgacgctc cccagatggt cgcaacctat acggcggaga tctacgaggt
     4741 cgatccgagc gaggtacaga tcctttccta tgggctgaac cactatggat gggtgtattc
     4801 ggttcaggtt cggggcattg aggttcttga tcagctgctt gattgccgaa gagaggagtt
     4861 tttcgccaag gagcccttct ata
//
LOCUS       DAOSLI010000092         4824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_15662_length_4824_cov_21.122667, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000092 DAOSLI010000000
VERSION     DAOSLI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15662_length_4824_cov_21.122667"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..676
                     /locus_tag="PLF67_08895"
     CDS             281..676
                     /locus_tag="PLF67_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE90166.1"
                     /translation="MNNLNSVLLEGNLVRDPELVRFEGNDSVLTKFSIASNRYYRNQK
                     GESVEEVLFMPVNVWGRLAENCSNYLSKGREVRVVGRLRQERWTDKEGNNRDRFVVVA
                     DHVEFRGERKKEDPVTLDEEVPFDQEVTF"
     gene            complement(684..1865)
                     /locus_tag="PLF67_08900"
     CDS             complement(684..1865)
                     /locus_tag="PLF67_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE90167.1"
                     /translation="MHPVFLFAGFFFFAVSYNLLGPLATNIMQTTGLSLSGSGTLVSF
                     QQIGSLISMAVSLLVMKRLKQGSVMKIGYLFLIVALVSIALSSTTTALFIFYLILGLG
                     SFLVDSASNATLASEYYEKRSLYIPLLHFCYSIGAIATGYLILPFKGVSWRWAYGLVG
                     VAMALILITGSVHRQATQKQSKKKRDAEPTKDVVGPITLLLKDRAFILYTLVIMFYMG
                     SQVICATWIPVYIETELGQSASITGTSLTVFWIGTALSRLIVGPIMNRGANPFTLSIW
                     GMFLAGISLVGATLSASIPIVLILVLLCGFFAGATIPMYVVIASTWYPRNTAFISLSY
                     IVSGTVGRMIFPYLVAKVASFSSLGFALMLSSLMLIISAFMILGVRSATRNRSESIHT
                     P"
     gene            2113..2439
                     /gene="trxA"
                     /locus_tag="PLF67_08905"
     CDS             2113..2439
                     /gene="trxA"
                     /locus_tag="PLF67_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOE90168.1"
                     /translation="MTVSEMIVTELNFKQEVLEADVPVLVDFWAPWCGPCKIIAPAVA
                     QIAQTYQGRLKVVKVNVDDAGSIANDYNINSIPTLMVFKDGEVVGQRLGAASLSVIEG
                     FVVQYL"
     gene            complement(2436..>2645)
                     /locus_tag="PLF67_08910"
     CDS             complement(2436..>2645)
                     /locus_tag="PLF67_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90169.1"
                     /translation="EVAQMAEELTQGLHWNGSMAKDAIDISKATATRSANRNGFIDTN
                     SANDWYVCATGNASFGVKNSTNRHH"
     assembly_gap    2646..2745
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2746..3523)
                     /locus_tag="PLF67_08915"
     CDS             complement(<2746..3523)
                     /locus_tag="PLF67_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90170.1"
                     /translation="MKYLVLLCIGVVCVGLVIGCTEHPPSLLNIFAQRDSSPPVLLDM
                     EMTAPAEACLRFNERIMIKKDAFTIEDNEIIDVSVWDEQLTIAFKKPLILGVFSTLEG
                     RVQDLAGNSMRFSIRLWAKNTRIPSLLINEFTTKGSENHPDRVELAVLKGGNLAGVTI
                     KTVNDSHTFGDHQVASGDYVVVAFSKGAVEAGSFLSKEQNGLSSNNGYICVHATPEWN
                     AEVLDCVLYSTKEATTHNGFGSKEVAQMAEELTQGLHWNGS"
     gene            3659..>4824
                     /locus_tag="PLF67_08920"
     CDS             3659..>4824
                     /locus_tag="PLF67_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90171.1"
                     /translation="MPTWMDVSNIELESIWDGEAMNLREGWTLSADGVVLDDDFTLPR
                     IVKDPDLSDKRVTLSRTIPSSYTAIRSLLFRTSQKRVKVYVDDRLTYSYDANIDNRRI
                     KLIGLINHFVRLEEGDQGKTLNIEMISNMPKSSGQFHEVYFGTRTAQIRRLFNYDGFS
                     LVVGVFLIINSLATAVIASRSLRNGKIYRGTLALSSIELCVGLWVCCGSMSTQLFIHN
                     QLLLVVLAMTALYMLPVCVTWFVLSMYSIPMRRFFMISTLFFPIAFVVVSALQLLGVI
                     TYITVLIPLAVLFFFYLLIILGAVMHAYRKGEKNAKPFLVATGFLFVSTVGELTLLIL
                     PHPTFINIQPLNIGVLIFGSILYSQILTQGVHFLEERGRKEYLQSLAYTDALTGL"
BASE COUNT         1154 a         1174 c         1277 g         1119 t
ORIGIN      
        1 gacctccaaa gctgcttgaa aatagaataa aagcaggcgt ggaaatatgc aaccttgtgg
       61 aaccgctttg cattcatgct tcatgtaacc gagggaaagg aatatcttct acgatatacc
      121 caacccacag atgatccgtg cattgatttg gaatgaaaga caacctgagt gaacccgttt
      181 taaagcaaac cggcaacacc catcccttaa gaggtatgtt ccccgatgaa agccgggaag
      241 cgatgcctgt gcattgcggc aggggagacg ggaggaaagg atgaataatc tcaattcggt
      301 gttgttggaa ggcaatctcg ttcgtgatcc ggagctggtc cggttcgaag gaaacgattc
      361 ggtgctcacg aagttctcca tcgcgtccaa tcggtactac cggaaccaga aaggggagag
      421 cgtcgaagaa gtgctcttca tgccggtgaa tgtatggggc aggcttgccg agaactgttc
      481 caattatctg agcaaggggc gggaagtacg agtcgtggga cggcttcgtc aggaacgctg
      541 gaccgacaag gaagggaaca atcgcgaccg gttcgtggtg gttgccgatc atgtggagtt
      601 ccggggtgaa cggaagaaag aggatcccgt cactctggac gaagaggttc ccttcgatca
      661 agaagttacg ttttaaaaaa cgctcaggga gtgtggattg attccgatcg atttcttgtc
      721 gcggaccgca ctcccagaat catgaaggcc gatatgatga gcatgagact gctgagcatc
      781 aaggcgaatc ccagcgagct gaaagaggcg acctttgcaa ccagatacgg gaatatcatt
      841 ctccctacgg ttccgctgac gatgtaggag agcgagatga atgccgtatt tcgtggatac
      901 catgtcgagg cgattacaac atacatggga atggtggctc cggcaaaaaa tccacagaga
      961 agaaccagaa tcaacacgat ggggatggag gcggacaggg tcgctccgac aagggagatt
     1021 cctgcaagaa acatgcccca aatggagagt gtgaaggggt ttgctccccg gttcatgatg
     1081 gggccgacga tcagccggga gagggccgtc ccgatccaga acacggtgag cgatgttccc
     1141 gtgatgcttg cggactgtcc cagctccgtc tcaatataga caggaatcca cgttgcgcag
     1201 atcacctggc ttcccatgta gaacatgatg accagagtat agaggatgaa ggctctgtct
     1261 ttcaaaagaa gggtaatggg gccgaccaca tccttcgtcg gttccgcatc ccgctttttc
     1321 ttgctttgct tttgagtggc ttgccgatga acggatcccg ttatgagaat gagcgccatg
     1381 gccactccga ccaatccgta tgcccatcgc catgaaacgc ctttgaacgg cagaatgaga
     1441 tagccggttg caatggcgcc gatgctgtag cagaagtgga gaagaggaat gtagagcgat
     1501 cgcttttcgt agtattcgct tgccagcgtt gcattcgagg cgctgtcgac gagaaatgat
     1561 cccaaaccca gtatcaggta gaagatgaac agggcggtag ttgtcgaaga gagcgcaatt
     1621 gatacaagag cgacgatcag aaacaggtag ccgatcttca tgaccgaccc ttgtttcaga
     1681 cgtttcatga caagcagcga gacggccatc gatatgagcg acccgatctg ctgaaagctc
     1741 accagcgtac cggatccgga aagggagaga ccggtggtct gcatgatgtt cgtggccagc
     1801 ggaccgagaa gattgtatga gaccgcaaaa aagaaaaatc ctgcaaagag aaacaccgga
     1861 tgcatagtgt cgactgtatc atggagcata agcgaatgca atcgatgggg atggagtgcc
     1921 tcaaacagaa gaaatcggga atggctgccg gaagcggaaa gctgagagca tatccaatgc
     1981 ggttacagct ggtgaatcct ataaaacaag gatattttgg caggattgat tatccaataa
     2041 aatgcacgga gggacacccc ggcgcccttt ctttttcaac ggtttgtatg tactattgag
     2101 ccgcaaggag agatgacagt gagtgaaatg atcgtgacgg agctgaattt caagcaggag
     2161 gttttggaag ctgatgtgcc ggttttagtc gacttctggg cgccgtggtg cggtccctgc
     2221 aaaatcatcg ctccggcggt agcgcagatc gcccaaacct accaaggccg actgaaggtg
     2281 gtgaaggtca acgtcgatga cgccgggtcg atcgccaacg attacaacat caattccatt
     2341 ccaaccttga tggtcttcaa agacggcgaa gtggtaggtc agcgcttggg ggccgcaagc
     2401 ctctcggtaa tcgaaggatt tgtcgtccag tatctttaat ggtggcggtt cgtggagttt
     2461 tttacaccga aactcgcatt gcctgtcgca cagacatacc aatcattcgc gctgtttgta
     2521 tcgatgaagc cgttacggtt agctgatcgg gtcgccgtag ccttgctgat atcgatcgca
     2581 tcctttgcca ttgacccgtt ccagtgcagt ccttgagtaa gctcttccgc catttgtgca
     2641 acctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgac ccgttccagt
     2761 gcagtccttg agtaagctct tccgccattt gtgcaacctc tttggaaccg aacccattgt
     2821 gggtcgtcgc ctctttcgtc gaatacagaa cacagtccag cacttcggcg ttccactccg
     2881 gagtcgcatg gacacagatg tatccgttgt ttgatgagag tccgttctgc tcctttgaaa
     2941 gaaaggatcc ggcctctacc gcgcctttac tgaaggctac gaccacgtag tcgccggagg
     3001 cgacctgatg atcgccgaag gtatgggagt cattgacggt cttgatcgtc actccggcaa
     3061 gatttccgcc cttgagaacg gcaagttcaa ctcggtcggg atggttttca ctgcccttgg
     3121 tggtgaactc gttgatcaac aatgaaggga tcctggtgtt ttttgcccac agccggatgg
     3181 agaagcgcat cgaattaccc gcaagatcct gaaccctgcc ctcgagggtg ctgaacacac
     3241 ccaagatcag gggttttttg aaggctatgg tgagctgctc atcccatacg ctcacatcga
     3301 tgatctcatt gtcttcgatc gtgaacgcat cctttttgat cataatgcgt tcattaaacc
     3361 tcaagcatgc ttcagcggga gcggtcatct ccatatcgag cagcaccgga ggagagctgt
     3421 cccgctgagc aaaaatgttg agcagggaag gggggtgttc agtacagccg atcacgagtc
     3481 ctacgcagac gacgccgata cacaaaagaa caaggtactt catatcccat attcgcaatc
     3541 catgacggtt ttacttgaaa atgatttaca gaatggattt gagggggcaa actatggtat
     3601 gagcaaaatc catacaggca tggtactggt gctagctgtt ctgatcatag gtgttttggt
     3661 gcctacatgg atggacgtgt ccaacatcga actggaatcc atttgggacg gtgaagcgat
     3721 gaatctgcgg gaagggtgga cgctgagcgc tgacggggtg gttctggatg atgatttcac
     3781 ccttcctcga atcgttaagg atccggattt aagcgacaag cgagttacgc tttcccggac
     3841 cattccctcc tcgtacacgg caatacgatc gcttctcttt cgaacctccc aaaagcgggt
     3901 gaaggtgtat gtcgatgatc gactcaccta ttcctacgac gcaaacatcg acaatcgccg
     3961 aatcaaactc atcggcctga tcaaccattt tgtccgtctt gaagaagggg atcaggggaa
     4021 aacgctgaat attgaaatga tcagcaacat gccgaaatca agcgggcagt tccatgaggt
     4081 gtatttcggc acccggaccg cccagattcg tcgtctgttc aactacgacg gtttttctct
     4141 cgtcgtggga gtcttcttga ttatcaactc cctggcaaca gccgtcatcg cctcgcgttc
     4201 actgagaaac ggtaaaatct atcgaggaac ccttgcactg agctcgatcg agctttgtgt
     4261 cgggctttgg gtgtgttgcg gaagcatgtc cacccagctt ttcatccaca atcaattgct
     4321 gctcgttgtc ttggccatga ccgcgctgta catgcttccg gtgtgtgtca cgtggttcgt
     4381 cttatcgatg tactcgatcc ccatgcgcag attcttcatg atcagcacgc tcttcttccc
     4441 gattgccttt gttgtcgttt ccgccctgca gctgctgggt gtgatcacct atatcaccgt
     4501 gcttattcct ttggcggttc tcttcttctt ttacctgctc atcatcctcg gggcggtcat
     4561 gcatgcgtat cgaaagggcg agaagaatgc aaaacccttt ttagtcgcca ccggctttct
     4621 ctttgtctcg accgtcggcg agctgaccct cctcatcctc ccgcacccga cgttcatcaa
     4681 catccagccg ctcaacatcg gcgtcctcat cttcggttcg attctctaca gccaaatcct
     4741 cacccaagga gttcacttcc tcgaagagcg gggaagaaag gaatacctgc agtcgctcgc
     4801 ctatacggat gctctgacgg gtct
//
LOCUS       DAOSLI010000093         4796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_15790_length_4796_cov_26.081417, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000093 DAOSLI010000000
VERSION     DAOSLI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15790_length_4796_cov_26.081417"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /gene="amrB"
                     /locus_tag="PLF67_08925"
     CDS             <1..409
                     /gene="amrB"
                     /locus_tag="PLF67_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOE90172.1"
                     /translation="ALVNHFSSLKALDEMSDFLLEEDEGLSIIASSDFTHYGPRFGFS
                     TLSEQEVKERDIQVARLLADGKIEEAFSFFTTSRHTICGWASAALVANIAKRKGMKGE
                     VAGYYTSNDIAGRDDGDFVAYATILWRQDERVS"
     gene            393..962
                     /gene="amrA"
                     /locus_tag="PLF67_08930"
     CDS             393..962
                     /gene="amrA"
                     /locus_tag="PLF67_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOE90173.1"
                     /translation="MKEYHDRLLILAREVLSASLGIGDAPMYELLKKRDDPFFTQKRG
                     VFVTLRTKDGALRGCIGSLLGYQPLVDQVVRLAHEAAFNDPRFKPVAAEELGGLVIEI
                     SLLTEPEPVSSWREIRLGVDGIIFEHGGRRAVFLPHVATEQGWDLETTLKHLSIKAGL
                     RANEYLDPHAAFEVFQATAFSERNDENKV"
     gene            946..1893
                     /gene="amrS"
                     /locus_tag="PLF67_08935"
     CDS             946..1893
                     /gene="amrS"
                     /locus_tag="PLF67_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOE90174.1"
                     /translation="MKTRCDFCYRECSLGEGQRGVCHIRVNRGGTIVTENYGEVVALA
                     LDPIEKKPLYHFRPGSYTLSAALFGCNLSCSFCQNYSISQKEFHQQRPNRYVSPEVFI
                     QQAVERNVSVSFTYSEPIVWQDYLLDCARMAKDEAIPTILVSNGTFSKASLTRILPFI
                     DAYSIDLKGDESFYRRYCNGSAKPVIAGLEALAKTSATVEVTTLLIEGEHTLSDVKEL
                     GTILSDLGIRVWHLSRYHPAYKLDLPPTGESFLQEALAAAKELKIPHVYGGNSRQDQD
                     TCCPECNALLIKRFPHQTKVFLKEGKCICSHQLYGDMES"
     gene            1925..2341
                     /locus_tag="PLF67_08940"
     CDS             1925..2341
                     /locus_tag="PLF67_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOE90175.1"
                     /translation="MRDTYIQRVHALRAKKYNCAQAVACTFSEEVGLEEELLFQIMEG
                     FGGGMGGHEATCGALSGAVAIISMLTSGGRIEANTKALTYERVSSLVKGFKEKAQSLV
                     CREILGEDDGTVLLGCEACIDEAVMLVAEFLTELKK"
     gene            2338..2970
                     /locus_tag="PLF67_08945"
     CDS             2338..2970
                     /locus_tag="PLF67_08945"
                     /inference="COORDINATES: protein motif:HMM:NF019443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOE90176.1"
                     /translation="MKGYPGRIIICGLDHEGEPIALYILTARSSSSRERILTVKADGL
                     WVEPTQNAQGGDPSLLYYRASFRRDGAIIIANGTHGERFTKTLAIEEALRDEFYEPDE
                     PIYTPRIAAVLDTGTARYAFASIRRSDDGSCTRSFFSFDALKAGQGHRIQTYEGDPKN
                     PRAFAGPPIPIALPKEDIAQTIFDELKPDLRIAVAVIRTDGYELINTHGG"
     gene            2973..4088
                     /locus_tag="PLF67_08950"
     CDS             2973..4088
                     /locus_tag="PLF67_08950"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOE90177.1"
                     /translation="MEGMNLKYGCNPNQKQAALLMESGTMPIQTLNGEAGYINLLDAL
                     NGWQLVKELKEATGFPSATSFKHVSPAGAAIGLPLNEEEARVFDAPEVLSPIAGAYVR
                     ARNADPLASFGDFVAISDECDLSLANVLKREVSDGIIAPSYAPEALELLKTKKRGRYV
                     ILQVKAEYEPPLMEKRTLFGMTLMQERNTVQLDSSLLKTIVTKKKELPPEVVRDLLIG
                     LVTLKYTQSNSVCYTKGGQAIGVGAGQQSRIACTRLAGEKADLFHLRFHPKLQDLKPD
                     TSATRQGRHLFIEEGIRNGTLLSKAEQRSCLKGISGVSLTSDAFFPFRDNIDRAAESG
                     VAYIAQSGGSTRDEEVIRAADEHNMVMCMTGIRLFHH"
     gene            4334..4756
                     /locus_tag="PLF67_08955"
     CDS             4334..4756
                     /locus_tag="PLF67_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE90178.1"
                     /translation="MKYYVTYNRNNPVSSFKSLFNDLWGDWESGSARIPPVDIYETEK
                     SYVIEAELAGYKQDEVKVNVDKHILTISSDKESLKDVDGKKSLVRERIYRKFERSFSL
                     PEGIDENKIEGEFTDGVLTLTLPKKKEVLPKTIEVKIK"
BASE COUNT         1148 a         1301 c         1359 g          988 t
ORIGIN      
        1 cgcccttgtc aatcacttct cctccctgaa ggccctggat gaaatgagcg acttcctgct
       61 tgaagaggat gaggggcttt caatcatcgc cagcagcgat ttcacccact acggtccccg
      121 cttcggtttc agcactctct ctgaacaaga agtcaaggag cgcgacatac aggtggcgcg
      181 cttgctcgcc gatggaaaga ttgaagaggc cttttccttc ttcaccacaa gccgtcacac
      241 gatctgcggg tgggcgagcg cggctctggt cgccaatatc gccaagcgaa aggggatgaa
      301 gggcgaggtg gcaggctact acacatcgaa tgacatagcc ggacgggatg acggggattt
      361 cgtcgcctat gcaacgattc tctggaggca ggatgaaaga gtatcatgac cgattgctga
      421 tccttgcccg ggaggtcctt tccgcttcgc tgggaatcgg agacgctccc atgtatgaac
      481 tgcttaaaaa gcgggatgac ccctttttca cccagaagag aggagtcttc gtgaccctca
      541 gaacaaaaga tggagcgctc cgggggtgca tcggcagcct gttaggctat cagccgctgg
      601 tcgaccaggt cgtccggctt gcccatgaag cggcgttcaa cgatccccgc ttcaagccgg
      661 ttgcagcgga agagctgggc ggtcttgtca tcgagatctc ccttctgacg gagcccgaac
      721 ccgtttcaag ctggagagag atccgactag gcgtcgatgg gatcattttc gaacatgggg
      781 gacgaagagc ggtgtttctc ccccatgtcg cgaccgaaca gggctgggat cttgagacga
      841 cgctgaagca cctttccatc aaagcagggt tgagggcgaa tgagtacctt gatccgcatg
      901 ccgccttcga ggtcttccag gcgaccgcat tcagtgagag aaacgatgaa aacaaggtgt
      961 gatttctgct accgggaatg ctccctcggt gaagggcagc ggggggtctg ccacatccgg
     1021 gtcaaccgcg gcgggacgat cgtcaccgag aactacggcg aggtcgtcgc cttggcgctc
     1081 gatccgatcg aaaaaaaacc cctctatcac ttccgacccg gttcatacac cctcagcgca
     1141 gccctcttcg ggtgcaacct ttcctgctcg ttttgccaga actactcgat cagccagaag
     1201 gaattccatc aacagcggcc gaaccggtac gtctccccgg aggtgttcat ccaacaggcg
     1261 gtcgaacgga acgtctccgt ctccttcacc tacagcgaac cgatcgtgtg gcaggactac
     1321 ctgctggatt gcgcccggat ggcgaaagat gaggcgatcc cgacgattct tgtctccaac
     1381 ggaacgttca gcaaggcctc tttgactcgg attcttccct tcatcgatgc gtactcgatc
     1441 gatctgaaag gcgacgagag tttttatagg cgttactgca acggctcggc aaagccggtc
     1501 atcgcgggct tggaggcatt ggcaaagacc tccgccacgg tggaggtgac caccctcctg
     1561 atcgaaggcg agcacaccct ttccgatgtg aaagagctcg ggacgatcct ctcggatctc
     1621 ggcatccggg tgtggcacct tagccgatac catcccgcct acaagctcga tcttcccccg
     1681 accggcgagt cgttcctgca ggaagccctt gccgcggcga aggagctgaa gattccgcat
     1741 gtctacgggg gcaacagccg ccaagaccaa gatacctgct gtcccgagtg caacgccctc
     1801 ttgatcaagc gctttcccca tcaaacaaaa gtgtttttga aagagggaaa atgcatctgt
     1861 tcccatcaac tctatggaga catggagagc tgaggtttga tatagtgagg gaaggagaat
     1921 catcatgagg gatacttata ttcaacgggt ccatgcgctg agggcgaaaa agtacaactg
     1981 cgcccaagcg gtggcgtgca cgttcagtga agaagtcggc cttgaagaag aactgctttt
     2041 tcagatcatg gagggcttcg gcggtggaat gggcggccac gaagcgacgt gcggagcgct
     2101 cagcggcgct gttgcgatca tcagcatgct taccagcgga ggccggatcg aagcgaatac
     2161 gaaagcgctc acctatgagc gggtttcctc tcttgtcaaa gggttcaaag agaaagccca
     2221 aagcctcgtc tgccgggaga ttctggggga ggatgacgga acggttctcc ttggctgcga
     2281 agcgtgcatc gatgaagcgg tcatgcttgt tgccgagttt ctcaccgagt tgaagaaatg
     2341 aagggatatc ccgggcggat catcatctgc ggcctcgatc atgaaggaga gccgatcgcc
     2401 ctctacatcc tgaccgcccg ctcctcctcg agcagggagc ggattctcac cgtcaaagcg
     2461 gacgggctct gggtcgaacc gacccaaaac gcccaaggcg gtgatccctc cctgctgtac
     2521 taccgcgcat cctttcggcg ggacggggcg atcatcatcg ccaacggaac gcacggcgaa
     2581 cgttttacaa agacgcttgc gatcgaagaa gccctcaggg atgagttcta tgaaccggac
     2641 gagccgatct atactccccg catcgcggcg gtgcttgaca cgggaacggc gaggtacgcc
     2701 ttcgcttcga tcaggagatc ggatgacgga tcgtgcactc gaagcttctt ctcctttgac
     2761 gcactgaaag ccgggcaggg acatcgaatc caaacctacg aaggggatcc gaagaatccc
     2821 cgagcgttcg caggtccccc cattccgatc gccttgccga aagaggatat cgcacagacg
     2881 atttttgatg agctaaagcc cgaccttcga attgcagtgg cggtgattcg aacggacggg
     2941 tatgaactca tcaacaccca tggaggctga tgatggaagg aatgaatctg aaatatggct
     3001 gcaaccccaa tcagaagcaa gcggccctct tgatggaaag cgggacgatg ccgattcaaa
     3061 cgctcaacgg cgaagcggga tacatcaatc tccttgatgc gctcaacggg tggcagctgg
     3121 tcaaagagct caaagaggcg acagggtttc cgagcgcgac ctcgttcaaa cacgtctcgc
     3181 cggcgggtgc ggcgatcggg cttcccctga atgaagagga ggcgagagtc ttcgatgcac
     3241 cggaggtgct ttccccgata gccggcgcgt atgtccgagc gcgaaacgcc gatccgcttg
     3301 cttccttcgg agatttcgtt gcgatcagcg acgagtgtga cctctccctc gccaatgtgc
     3361 tcaaacggga ggtgagcgac ggaatcatcg ctccttccta tgccccggaa gcgctcgaac
     3421 ttttgaaaac aaagaaacgg gggcgttacg tcatccttca ggtgaaggcc gagtatgaac
     3481 cgcccctgat ggaaaaacga accttgttcg gaatgaccct gatgcaggag cggaataccg
     3541 tccaactcga ctcctcgctc ctgaagacga tcgtcacgaa gaagaaagag cttccccccg
     3601 aagtggtccg ggatcttctc atcggtctgg ttactctcaa atatacccaa tccaactcgg
     3661 tgtgctacac caaagggggc caggcgatcg gagtgggagc gggacagcaa tcgcgaattg
     3721 cctgcacccg tcttgcaggg gaaaaagcgg atctcttcca tctccgcttt caccccaagc
     3781 tgcaggatct caagccggac acgtccgcca ctaggcaagg ccggcatctc ttcatcgaag
     3841 agggaattcg aaacgggacg ctgctttcaa aagcggagca gcgatcctgt ctgaagggca
     3901 tttccggggt aagcctgacc agcgacgcgt tctttccctt cagagacaac atcgaccgag
     3961 cggccgaaag cggtgttgcg tacatcgccc agagtggcgg ctccacccgg gacgaggagg
     4021 tcatccgggc ggcggatgag cacaacatgg tgatgtgcat gacggggatt cggctgtttc
     4081 atcattgagt tgggtcatga agaaccaaaa aagggtcttt atagcccaag tgtgtcgttt
     4141 tgattcaaat atgccgcatt tctttcttct tcaaggatag tttcctgttt gtttcatatc
     4201 tcttttttcg gaaaaagcaa tataaatcat tatatagtaa tagtttaaat ataacataac
     4261 gatttattcg ccgaggttgg cacgccattt gcattatatt gagtagaaca acaatgcaca
     4321 gtggaggtgc cttatgaaat actacgtaac ctataatcga aacaacccgg tatcgagctt
     4381 caagtccttg ttcaacgacc tttggggcga ctgggagtcc ggcagtgccc ggattcctcc
     4441 ggtggatatc tatgagaccg agaagagcta tgtcattgaa gcggagcttg ccggctacaa
     4501 gcaggatgaa gtcaaagtca acgttgacaa gcacattctg acaatctcca gcgacaagga
     4561 gagcctcaag gacgtcgatg gaaagaagag ccttgtccgg gagcgaatct atcggaagtt
     4621 cgagcgttcc ttcagcctgc ccgagggaat cgatgagaac aagatcgaag gcgagttcac
     4681 cgacggtgtc ttgacgctca cccttcccaa gaagaaggaa gtccttccca aaacgatcga
     4741 agtgaagatc aagtaagaaa aaacggatca gctttgtaca ttcaggcagc cgggag
//
LOCUS       DAOSLI010000094         4703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_16183_length_4703_cov_23.364458, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000094 DAOSLI010000000
VERSION     DAOSLI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16183_length_4703_cov_23.364458"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1294
                     /gene="manA"
                     /locus_tag="PLF67_08960"
     CDS             95..1294
                     /gene="manA"
                     /locus_tag="PLF67_08960"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOE90179.1"
                     /translation="MNIVEISGVIKDHTWGNTSSIPSLLGVEEDGKPKAELWFGTHPD
                     GMATVVETGETLQNLLASDTRHWLGEAHLATFGEELPLLLKVLAIGEPLSIQVHPNKK
                     QAEKGWAKEEHLRSRLHKEQWNYKDPNRKAEVGYALEPVTALSGFRPLKEIRAWLKRL
                     IPSALQTFFSYLDEEGDEDGRIRRFFEALYTLEKKDLKIVLDELFLSLEDLPLESADR
                     SFLTEKGIVEYCRTFYPLDPGLLAPFFLNVVHLDIGEGLFIPPGVLHAYMKGVVLEVM
                     SNSDNVLRGGLTPKKVDLHELFSIVEVIGKPAELTRQLIGRSGRVHLLTPTEEFHLMV
                     LHSGTYEIDERLSIELLFQTEGESTLKGAGEERRLKKGSCHVVAAGLGSYTLHVEGTL
                     FIADVPR"
     gene            1335..2609
                     /locus_tag="PLF67_08965"
     CDS             1335..2609
                     /locus_tag="PLF67_08965"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOE90180.1"
                     /translation="MDRYLSVLQREMRVAMGCTEPAAAALCAAKASELLGENPVRLHV
                     SASGEMLKNAMGVGIPNTALKGLKAAVALGAAIADIQDGLNILSTIDEAVISKAEGFP
                     VSLTIVKDVPSLYIQVEAVGLNHFARATISGEHERFSELVKDEEVLLSLPLDGCSATL
                     EEVDEVILSKSTLADILSWVEEAPPEAHALVLNALETNLSIARHSLESEYGLTVGKTI
                     FTDCEKELQSLDQAFKRGSALAAAASDARMAGCPLPVVINSGSGNQGITLTVPLAPVA
                     EYLSSSDEELARALLVGQLVGLSLTAHKSRLSALCGAFTAAIGTACSLVYLLGGSQKQ
                     MDMAFNTMVGNLTGIICDGAKGTCALKIYSCVEAAHLAVKLALKGVGADGKSGIVGMS
                     SQESIDFLARLMKEGMGKTEETILSIMVGKEQ"
     gene            2606..3199
                     /locus_tag="PLF67_08970"
     CDS             2606..3199
                     /locus_tag="PLF67_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOE90181.1"
                     /translation="MTYTAIDFETANSYPGGGASVALVRFDQDGQKLEEFYTLLRPKH
                     SYFDPGMTAVHNLDEGECLASAEFDRVWPRMRKFIGSDLLVAHNAVFDMGVLKGSFEA
                     YDIEAKEMNYLCTLTIARRIWPNLLSYRLSYLVDYFGMEYNAHHALDDALMCGRIFHK
                     QCAGHLDSMFSLRRFLITYKVEPKVIVHHRTGGDFFL"
     gene            complement(3159..4058)
                     /locus_tag="PLF67_08975"
     CDS             complement(3159..4058)
                     /locus_tag="PLF67_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOE90182.1"
                     /translation="MQASILRFLHRIATPFLDLLANGASFFGEETFVIAIVLLVFWNI
                     NKQKGFALYMNVLTSVLVMGILKAVVRAPRPFVVLDDIAGKRMGTATGYSFPSGHTTT
                     AASFYTSLALLLKKRICSIIAAIMIVLVGVSRLYLGVHWPNDVFAGLLIGVSISFLLY
                     RWSLQLFEDRVRLVRFSIRYGLIATFVSLILSVLLNMELVDPVAFNDLMKTLALAGAG
                     LLGFGFEEQIVRYRVEASLTKKILRYLLGMGVVIAIIASKALFPPSIYAISSFIRYSL
                     VGVWATVLFPITGKSLHLFDDER"
     gene            complement(4096..4671)
                     /locus_tag="PLF67_08980"
     CDS             complement(4096..4671)
                     /locus_tag="PLF67_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE90183.1"
                     /translation="MKTEIAPLHSKLLHTPRLLLRPPTMADSEAVFAWSSDPEVTRYL
                     RYQTHNSITHTRKIVKSWITQAKDPYFFFWIIEEISTEQAVGSIGIQVISFNDNHGEV
                     GYCFNRSVWNKGYCTEALKEVLRFGFEEARFNRIQASHSTNNPASGRVMEKAGMIREG
                     GPMKDFYKSLQSGYQDTYLYGITKAAWRLLH"
BASE COUNT         1110 a         1154 c         1356 g         1083 t
ORIGIN      
        1 ggggcgatgt atctcatgcc gaaatatttc aaataatcct ctcaaagctt tctctttgct
       61 catggctcct ctatactcac agtgtggagg agccatgaat attgttgaga tcagcggtgt
      121 gataaaagac catacatggg ggaatacctc atcgattcct tccctgctcg gagttgagga
      181 ggatggcaaa cccaaagctg aattgtggtt tggaacccac ccggacggaa tggcgacagt
      241 agttgaaacg ggagaaactc ttcagaacct tcttgcttcc gacacccggc actggctggg
      301 agaagcccat ctggctacct tcggcgaaga gcttccactg cttttgaaag tcttggcgat
      361 cggggagccc ctgtcgatcc aggttcaccc caacaagaag caggcggaga agggatgggc
      421 aaaggaagaa cacctgcgca gtcgccttca taaagaacag tggaactaca aagatcccaa
      481 ccggaaagca gaggtcggct acgctcttga gccggttacc gctttaagcg ggtttcgtcc
      541 gctcaaggag attcgagcat ggctcaaacg attgatccct tccgctcttc agactttttt
      601 ctcgtatctt gatgaagaag gtgatgaaga tggacgtatc cgtcgcttct ttgaagcgct
      661 ctacaccttg gaaaaaaagg acctgaaaat cgtcttggat gagttgtttc tttccttgga
      721 agatcttccg cttgagagcg ccgatcgatc attcctgacg gagaaaggaa tcgttgaata
      781 ctgtcgaaca ttttatccgc tcgatcccgg cctgttggct ccgttcttcc tgaacgtggt
      841 tcatttggat atcggtgaag gattgttcat cccgccggga gttctacacg cttatatgaa
      901 aggggtcgtc cttgaggtga tgagcaattc ggacaacgtt cttcggggag gattgacgcc
      961 caaaaaagtg gatctccatg aactgttttc aatcgttgaa gtgattggaa agcccgctga
     1021 attgactcgg caactcatcg gacgcagcgg acgagtccat cttctcactc ccaccgaaga
     1081 gtttcatctg atggttctcc actcgggaac ctatgagatc gatgaacggc tctccatcga
     1141 actgcttttc cagacggaag gagaatccac gctcaaggga gcaggcgaag agcgaagatt
     1201 gaagaagggc agttgccacg tcgtcgcagc gggattgggc agctatacat tacatgttga
     1261 aggaaccctg tttatcgccg atgttccccg ctagggtgtt ggctcggtgc ccgtcaggtg
     1321 atactatcgg ttttatggat agatatttat cagtgttgca gagagagatg cgcgttgcga
     1381 tggggtgtac cgaacccgcc gccgcagcgc tctgcgcggc caaagcttcc gagcttctcg
     1441 gtgagaatcc tgtacgtctg cacgtttcag caagtggaga aatgctgaaa aatgcaatgg
     1501 gggtggggat tcccaacacc gcgttgaagg gtctcaaagc ggcggtggcg ctcggagcgg
     1561 cgatcgccga tatccaggac gggttgaaca tcctctccac gatcgatgag gccgtcattt
     1621 cgaaggcgga gggatttccc gtctccctga ccatcgtcaa ggatgtgcct tccttgtata
     1681 tccaagtcga agcggttggc ttaaaccact tcgcacgagc aacaatcagc ggcgaacacg
     1741 aacgcttcag cgagctggtg aaggatgagg aagtgctttt gtcattgcct ttggacggat
     1801 gctccgctac cttggaagag gtggacgagg tgattctttc gaaatccacc ttggcggata
     1861 ttctctcctg ggttgaagag gcgccgcctg aagctcatgc gctggtgctc aacgctcttg
     1921 agacgaacct ctcgattgcc cggcattcgc ttgaaagcga gtacggtttg acggtgggca
     1981 aaacgatttt cacggactgt gagaaagaac tgcaaagctt ggatcaagcg ttcaagcggg
     2041 gatccgcttt ggctgccgca gcaagcgacg ctcgaatggc cgggtgtccc cttccggtgg
     2101 tgatcaacag cggcagcggc aatcagggga tcaccttgac cgttcccctt gctcccgtgg
     2161 cggagtatct gagctcaagc gatgaagagc ttgcccgggc tttgctggtc ggccagctgg
     2221 tcggtctttc gctcaccgca cataaaagta gattgagtgc cctgtgcggg gcgttcaccg
     2281 cggcgatcgg gactgcctgt tccctggtgt atctgcttgg gggatcccag aagcagatgg
     2341 acatggcgtt caacacgatg gtgggaaacc tcaccggaat catctgcgac ggagcaaaag
     2401 gaacgtgtgc tcttaaaatc tactcctgtg tcgaagcggc tcaccttgcg gtgaagctcg
     2461 ccctcaaagg ggtgggggcg gatggaaaaa gcgggatcgt cggcatgagc agccaggagt
     2521 cgatcgattt tcttgccaga ttgatgaaag aaggaatggg caagacggaa gagacgatcc
     2581 tttcgatcat ggttgggaaa gagcaatgac gtacactgcg atcgatttcg agacggcgaa
     2641 cagctatccc ggcggcggag caagcgttgc tctcgttcga tttgaccagg atggacagaa
     2701 gcttgaggag ttttacacgc ttcttcgtcc caagcattcc tattttgacc cggggatgac
     2761 ggcggttcac aatcttgatg aaggtgagtg tctggccagc gccgagtttg acagggtgtg
     2821 gccgaggatg cgcaagttca tcggaagcga ccttcttgtc gcccataacg cggtcttcga
     2881 catgggcgtc ctgaaaggat cgtttgaagc ctacgacatc gaggccaagg aaatgaatta
     2941 cctgtgcact ttgacgatcg cccgaagaat ctggccaaac cttctcagtt accggttgtc
     3001 atatctggtc gattacttcg ggatggagta caacgcccac catgccttgg atgatgcatt
     3061 gatgtgcggc aggatcttcc ataaacagtg tgccggtcat ctcgattcga tgttcagcct
     3121 tcgtcggttt ctgatcacct acaaggttga gccgaaggtc atcgttcatc atcgaacagg
     3181 tggagacttt ttcctgtgat cgggaaaagc accgtcgccc agactcctac gaggctgtag
     3241 cggataaagg agctgatcgc gtagatcgag ggcgggaaga gggccttcga ggcgatgatg
     3301 gcgatgacca cccccattcc aaggagatag cgaagaatct tcttcgtcag cgatgcttcg
     3361 actctgtagc gaacaatctg ctcttcaaat ccaaagccga gcaacccggc tccggcgagt
     3421 gcaagggttt tcatcaggtc attgaaggcc acggggtcga ctaattccat attcagcaga
     3481 acggaaagaa tcaaggagac aaaagtggcg ataagaccgt agcggatgct gaaacgaacc
     3541 aggcgcaccc gatcctcaaa aagctgaaga gaccaacgat agaggaggaa actgatgctc
     3601 accccgatga gaagacctgc gaacacatca ttcggccaat gaacaccgag atagaggcga
     3661 ctcactccga cgagaacgat catgatcgcg gcgatgattg aacatattct tttcttcagg
     3721 agaagggcga gggaggtgta gaaggaggct gcggtagttg tgtgcccgct cgggaaggag
     3781 tagcccgtag cggttcccat tcgcttgccg gcgatgtcat ccaggacgac gaacggtctg
     3841 ggagcccgga cgaccgcctt gaggattccc atgaccagga cagaggtgag gacgttcata
     3901 tagagggcga aacctttctg cttattgata ttccaaaaga ccagaagaac gatcgctatg
     3961 acgaaggtct cttcaccaaa gaagcttgca ccattggcaa gcaggtccag aaacggggtt
     4021 gcgatccgat gtaaaaagcg cagaattgaa gcttgcatat atcctcctgt gtggaagtat
     4081 agaagcgccg tgtgctcagt gcaagaggcg ccacgccgct ttggtgatgc cgtagaggta
     4141 ggtgtcctga tagccgctct gcaagctttt gtaaaaatcc ttcatcgggc cgccttcccg
     4201 gatcatcccc gctttttcca tcacccggcc gcttgccggg ttgttggtgg agtgggaagc
     4261 ctgaattcgg ttgaagcgcg cctcttcgaa tccgaagcga agaacctctt tcaatgcttc
     4321 ggtgcagtac cctttgttcc agacggaacg attgaagcaa tagccgactt ccccgtggtt
     4381 gtcgttgaac gagatgacct gaatccctat cgatccgacg gcttgttctg tcgaaatctc
     4441 ttcaatgatc caaaagaaga aatagggatc tttggcttgg gtaatccacg atttgacgat
     4501 cttgcgggta tgggtgatcg agttgtgggt ctgatagcgc agatacctgg tcacttcggg
     4561 atcgctcgac caggcgaaga ccgcttcgct gtcggccatg gtgggcggcc tcagcagaag
     4621 cctgggggta tggagaagct tggagtgaag cggtgcgatc tcggttttca tcatgatggc
     4681 ttagcgtata ctcttgctca att
//
LOCUS       DAOSLI010000095         4607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_16562_length_4607_cov_20.680580, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000095 DAOSLI010000000
VERSION     DAOSLI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16562_length_4607_cov_20.680580"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PLF67_08985"
     CDS             <1..1133
                     /locus_tag="PLF67_08985"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOE90184.1"
                     /translation="KRFLLIPDSFKGTMSSAEICSIIEQGLKPHYPDANIRSIPVADG
                     GEGSVDAFLEALGGEKRHITVQGPFGEAMESFYGVLPGSVAVIEMAACAGLPLVGDTL
                     RPDLTTTYGVGELILAAAKEGCTRIIVGLGGSATNDGGCGAAAACGVTFRNKEGESFI
                     PTGGTLGEIASIDRSTRNPILDRVTIITMCDIDNPFYGETGAAYIFGPQKGADPEMVR
                     RLDANLESLAKVIESDLDLDVQAIPGSGAAGGMGGGMAAFFDSELQMGIETVLDAVHF
                     DELLKETDMVITGEGKIDGQSLRGKVVIGVGRRTKKSGVPVIALVGDIADDADGAYEE
                     GVSAIFSINRLAIPFKEAKLRAKTDLLKTVDNLARFLKTMKW"
     gene            complement(1137..2048)
                     /locus_tag="PLF67_08990"
     CDS             complement(1137..2048)
                     /locus_tag="PLF67_08990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOE90185.1"
                     /translation="MNTPVIDLHIDTIYALFNRYSSGDLRSNTGHVDLQRMKTLQGIT
                     SCFALFVDGQETPGKWEAVSELHRSFLEQMRGNGETIDHVATGEKTEGCHALLSVEEG
                     GVLEGDLARLEILHSWGVRLMTIGWNWENELGFPHYQKGALKPFGHQVVKRMEELKIL
                     VDVSHLNDEGINDIVTIAKRPVVASHSNCRRVCNHSRNLSDELIGKIAETGGVVGLTF
                     CPPFVIRQSTHTSVEALAAHCKHFYKVGGSEILALGTDYDGFTGTCEIRGYQDLHILH
                     SALERVGFSSSVLDGFWYRNARRVLDA"
     gene            2153..>2468
                     /gene="trxA"
                     /locus_tag="PLF67_08995"
     CDS             2153..>2468
                     /gene="trxA"
                     /locus_tag="PLF67_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOE90186.1"
                     /translation="MIKHVNKSTYESEVLKSKAPVIVDFWAPWCGPCRMQAPILEDLA
                     KQYTADQLKIVKVNVDEERELAMAYGIQSIPTLLIYKNGEVVNKAIGVRDGATLKRAV
                     GLA"
     assembly_gap    2469..2568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2787..4076
                     /gene="trpB"
                     /locus_tag="PLF67_09000"
     CDS             2787..4076
                     /gene="trpB"
                     /locus_tag="PLF67_09000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOE90187.1"
                     /translation="MANQLTKTINTKDLPNNEGYFGEYGGAYLPPNLEEVMKEIDRAY
                     RKIVEDPAFIEALMDLRKHYVGRPSPIYHAKRLSEAVGGAQIYLKREDLNHTGAHKIN
                     HCLGEVLLAKAMGKKKVIAETGAGQHGVALATAAALMGLECDIYMGSIDVRKEAPNVS
                     RMKILGAKVIEVERGTQSLKDAVDAAFEAYLGDPVDQIYCIGSVVGPHPFPMMVRDFQ
                     SIVGIEAKEQIQEMIGRLPEAIVACVGGGSNAMGIFSAFLDDTDVAIYGVEPAGKGLD
                     TPNHAATITKGSVGILHGFKSLVLQDEQGEALPVYSIASGLDYPGVGPQHSFLHSIKR
                     VIYESITDREAVEAFYGLSRMEGIIPALESSHAVAYAIKLAQTMSPNEVILVNLSGRG
                     DKDIDFVLEHYPLEEYEPEAKNGRDGFKEFLAHIKTK"
     gene            complement(4083..>4607)
                     /locus_tag="PLF67_09005"
     CDS             complement(4083..>4607)
                     /locus_tag="PLF67_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOE90188.1"
                     /translation="IGDVGMYLSLALPGTLLFEAETEAYPSLALSYGSVIGAGYLVRT
                     VLKEAITAQRPYVGYPDAPPPSSDDYKSFPSGHALMTFASAAYLQTAVCLWHSDSTLL
                     KSMAVANWILAAATSTLRVVGGSHHVHDVLAGAAIGGGLGFLGPFITDRLQKRDRQAP
                     RVLIGQATGVLVSY"
BASE COUNT         1069 a         1253 c         1282 g          903 t
ORIGIN      
        1 tgaaacgctt tcttctgatc cccgattcct tcaagggaac catgagttcc gccgagatct
       61 gctcaatcat tgaacagggt ctcaaacccc attatcccga tgcaaacatt cgttcgatcc
      121 ccgtcgccga cggcggtgaa ggaagcgtcg atgcatttct ggaggccctc ggtggagaga
      181 agaggcatat caccgtccaa ggcccgtttg gggaagcgat ggaaagcttc tacggggtgt
      241 tgcccggtag cgtcgcggtc atcgaaatgg cggcatgcgc gggccttccc ctcgtcggcg
      301 acacgctgag gccggatctg acgacgacct acggcgtcgg cgagctgatc ctcgccgctg
      361 cgaaggaagg atgcacccgc atcatcgtcg gtcttggagg atcggcgacc aacgacgggg
      421 ggtgcggagc cgccgccgcc tgcggcgtca ccttccggaa caaggaaggc gaatccttca
      481 tccccaccgg aggcactctc ggagagatcg cttcgatcga ccggtcaacc cggaacccga
      541 tcctcgaccg ggtgacgatc attacgatgt gcgacatcga caacccattt tatggtgaga
      601 cgggtgcggc atacatcttc gggcctcaga agggggcgga tcccgagatg gtgagacgtc
      661 tggatgcaaa tctcgaatcg ctggccaagg taattgaatc cgacctcgac cttgatgtcc
      721 aagcgattcc cggtagcgga gccgccgggg gcatgggtgg cggaatggcg gcattcttcg
      781 acagcgaact gcaaatggga atcgaaacgg ttctcgatgc cgtccacttt gacgagctgc
      841 tcaaagaaac ggatatggtc atcaccgggg aaggaaagat cgacggccaa tcccttcgcg
      901 gcaaagtggt gatcggggtc ggccgacgaa cgaagaaaag cggtgttccg gtgattgcgc
      961 tggtcggcga tatcgccgat gacgcagacg gagcgtatga agagggagtc agcgcgatct
     1021 tttcgatcaa ccgcctggcg attcccttca aggaagcaaa gcttcgagcc aaaacggacc
     1081 tgctcaaaac ggtcgacaac ctcgcccgct tcctcaagac aatgaagtgg tagagttcag
     1141 gcgtccagca cccgccgggc gttcctgtac caaaacccgt ccagaacgga cgaagaaaat
     1201 ccgacccgct cgagagccga atggaggata tggagatcct gatatcctcg aatctcgcac
     1261 gttccggtaa atccgtcgta gtcggttcca agagccagaa tctcggagcc gccaaccttg
     1321 tagaaatgct tgcagtgggc tgcgagcgct tcgactgaag tatgggtcga ttgccgtatt
     1381 acaaaaggcg gacaaaacgt cagcccgacc accccgccgg tttcagcgat ctttccgatc
     1441 agctcatcgc tgaggttccg gctgtggttg cacacccttc gacagttgga gtgactggcg
     1501 acgaccggcc gtttggcaat cgtgacgata tcgttgattc cctcatcgtt gaggtgggac
     1561 acgtcgacaa ggatcttcag ttcctccatc cgcttgacga cctgatgccc gaagggcttg
     1621 agagcccctt tctgatagtg gggaaaacca agctcgtttt cccaattcca cccgattgtc
     1681 atcaggcgca ctccccatga gtggagaatc tccaatcggg caagatcccc ttcaaggact
     1741 cctccttcct cgacgctgag aagggcgtgg catccttccg tcttctcacc cgtggcaaca
     1801 tggtcgatcg tctccccgtt tccccgcatc tgctcaagaa aggatcgatg cagttcactc
     1861 acggcctccc acttcccggg agtctcctgc ccgtcaacaa aaagggcaaa gcagctcgtt
     1921 attccttgca gggttttcat tcgctggagg tcgacatgac cggtgttcga tcgaagatcc
     1981 ccgctcgaat accgattgaa aagagcatag atcgtgtcga tgtgcaagtc gataaccgga
     2041 gtgttcaccg tgaagcctcc caagtagttg tcgctagcaa gtaaatgtag tatcttactg
     2101 gtcgtagcag atgaatacac tactatggta cacaacgtaa caggagaatg gaatgattaa
     2161 acatgtaaac aagagcacct acgaaagtga agtgctcaaa agcaaggcac cggtcatcgt
     2221 cgacttctgg gcaccttggt gcggaccctg ccggatgcag gctccgattc ttgaagatct
     2281 ggcgaaacaa tatacggctg atcagttgaa gatcgtcaaa gtcaacgtcg atgaagagcg
     2341 tgagctcgcg atggcgtacg ggatccaatc gattccgacc ctgctcatct acaagaacgg
     2401 cgaagtggtc aacaaagcga tcggcgtccg cgacggcgcc accctgaaac gagccgtcgg
     2461 gcttgcttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa agcgatcggc
     2581 gtccgcgacg gcgccaccct gaaacgagcc gtcgggcttg cttaagatta tcttttatcg
     2641 atgcccccac ttttttttgt gggggtattt ttttgcaata aaagaagtaa gaaactcatg
     2701 tttcttttta tagaaacttt tttcaaaaac ctgttgacat tgtgtttcta tgtacagtaa
     2761 catccctgct attaaggaga tttctcatgg ctaatcaact gacgaaaaca atcaacacga
     2821 aagacctgcc aaacaacgaa gggtacttcg gcgaatatgg cggagcatat cttcctccaa
     2881 acctggaaga ggtgatgaag gagatcgacc gcgcctatcg aaagatcgtc gaagatcccg
     2941 ctttcattga agcgctgatg gacctgcgca agcactacgt tgggcgcccc tccccgatct
     3001 atcatgccaa acggctcagc gaagcggtcg gaggagcgca gatctatctg aaacgggaag
     3061 atctcaacca taccggcgcc cataagatca accactgtct cggcgaagtc ctccttgcca
     3121 aagcgatggg caaaaagaaa gtgattgccg aaaccggagc cggccagcat ggcgtggccc
     3181 ttgccaccgc ggcggcgttg atgggcctgg agtgcgacat ctacatgggg agcatcgatg
     3241 tccgaaagga agcgccgaac gtcagtcgga tgaagattct cggtgcgaag gtcatcgagg
     3301 ttgaaagagg aactcaatcc ctcaaagacg ctgttgatgc cgcctttgaa gcgtatctgg
     3361 gcgatccggt cgatcagatc tactgcatcg gctcggtagt cggcccccac ccctttccga
     3421 tgatggtccg cgatttccaa tcgatcgtcg gcattgaggc gaaagaacag attcaagaga
     3481 tgatcgggcg cctgcccgag gcgatcgtgg catgtgtcgg cggcgggtcg aatgcgatgg
     3541 gaatcttcag cgcgttcctg gacgacacgg atgtggcgat ctacggagtc gagccggcgg
     3601 gaaagggatt ggacacgccc aaccacgctg cgacgatcac caagggaagc gtgggaattc
     3661 ttcacggctt caaaagtctc gttcttcagg acgaacaagg ggaagctctt cccgtctatt
     3721 cgatcgccag cggcctggat taccccggcg tcggaccgca gcactcattc ctgcactcga
     3781 tcaagcgggt gatctatgaa tcgatcaccg accgcgaagc ggtggaggcg ttctacggcc
     3841 tttcccggat ggaaggaatc atccccgccc tcgaatcaag ccacgccgtg gcgtatgcga
     3901 tcaagctggc tcaaacgatg agtcccaacg aagtcattct ggtaaacctt tcaggccgag
     3961 gggacaagga tatcgacttc gtgctggagc actatccgct ggaagagtat gaaccggaag
     4021 caaagaacgg ccgggacgga ttcaaggagt tcctcgccca catcaagacg aaatagcccc
     4081 tgtcaatagg aaacaagcac cccggtagcc tgaccgataa gcacccgggg tgcttgtctg
     4141 tcacgctttt gcagtctgtc ggtgatgaag ggcccgagaa agccgagacc tcccccgatc
     4201 gccgctccgg ctagaacgtc atggacatgg tgggagcctc caacgacccg aagggtcgag
     4261 gtggcggcag ccagaatcca gttggcaacc gccatcgatt tcaacagcgt cgaatccgaa
     4321 tgccacaggc acaccgccgt ctgcaggtac gcggcggaag cgaaggtcat cagggcgtgt
     4381 ccgctgggaa agctcttgta atcgtcgctg ctgggaggcg gggcgtcggg atatcccaca
     4441 tagggccttt gggcggttat cgcctctttc aaaacggttc ggacgagata gccggcaccg
     4501 atcacagagc catagctgag cgccaaggag ggatacgctt ccgtttccgc ctcaaagaga
     4561 agggttcccg gaagcgccag gctcaggtac atccccacat caccgat
//
LOCUS       DAOSLI010000096         4598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_16604_length_4598_cov_24.811358, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000096 DAOSLI010000000
VERSION     DAOSLI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16604_length_4598_cov_24.811358"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="PLF67_09010"
     CDS             complement(<1..1615)
                     /locus_tag="PLF67_09010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE90189.1"
                     /translation="MDYQAKDFGKLLKNNALTMRRLFQSTDTNCMRVYDRNMEEFPVT
                     VDLWAQWVRITDYSEEGLDDRKRDLVMDLAGRMLYVPIEKVVYHHRRKRTGREQHDVQ
                     EPVSTLVEVMEQGLVFTVDLTKRVDTGLFLDHMPTRALVGSMSYGLRVLNLFSYTGSF
                     SVYAARGGAVQVDSVDLSATYTAWAEANLAANGFFGPTYRCINADAFEYVKDALDEKR
                     IYDLIIFDPPSFSNSRKMEHDFDIVRDYVRWLHALSALLKKGGTIIVSSSLGGFSLDK
                     SALGGYTIEERTQDLLAPGFTRRSGTARTWVLTKERNHTIPAIFRNSRKNTETAHNEA
                     QMDEEEMSKEEEKNKTEVKEATRSEEIMDQAFLSEEEDDVWTLVWDDEPEQKAEVKKE
                     RARRSESRRSSSSDRSRRDDSRPRRFDDRPRRDYDDRPRRYDDRPRREYDDRPRRYDD
                     RPRWDYDDRPRRYDDRPRREYDDHPRRYDDRPRREYDDDRPRRYDDRPRREYDDRPRR
                     YDDRPRREYDDRPRRYDDRPRREYDDHPRR"
     gene            1600..2151
                     /locus_tag="PLF67_09015"
     CDS             1600..2151
                     /locus_tag="PLF67_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90190.1"
                     /translation="MLDNPYALATLSATQCICYYEKKLVRQGHTMDDDAHKFIQELET
                     RHGSKLSWRTYATWYATTKGVAREFGVFLYKVNDSFFFEDFERQPALFGFSLRPRKNR
                     EPFVKYEDSFACTGVESVRTVSRSNALKIIRQPSHPVKDASWFDKAFRQVVEMVLLKD
                     GTVHFFEVMDRKEFRRRLNDGSV"
     gene            2138..3475
                     /locus_tag="PLF67_09020"
     CDS             2138..3475
                     /locus_tag="PLF67_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOE90191.1"
                     /translation="MGAFKAYDIRGIYNVDFNKDTVYKIGYFLPKLLKSKAVLVGRDV
                     RTSSDEIFEALSRGIMDSGADVWNIGLATTPMVYFATVHFKADASVQITASHNPAEYN
                     GLKISRTNAVPVGSDSGLKELEAMVLNDEIVPSRKKGKIIRKEARNAYIDFLKAFTPD
                     TSDLNLSIDFSHGMANLIVKDLLGENHHYLYDHLDGTFPAHEPNPLEVENTYDLRDAV
                     IANKSDIGIIYDGDADRVMFLDENGRFLQPDYITSLLGLYYLKKEKGRVLVDIRTSRS
                     TVEYLESLGAEVHVWKVGHAFAKNKLRELGAIFGGELAGHYYFRDFFNCDSGILASLL
                     VLQAVKMLKTEGQTIGSFIDSIVRWANSGEVNYTLNEKDEAMTALYERYVKNDSPTLV
                     MDFDGYRVEFKDWWFNVRKSNTEPYLRLVVEAATQALLDEKLADLSTIITRFS"
     gene            3488..4474
                     /gene="galE"
                     /locus_tag="PLF67_09025"
     CDS             3488..4474
                     /gene="galE"
                     /locus_tag="PLF67_09025"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOE90192.1"
                     /translation="MKILLVGGAGYIGSHVALQFLERGDQVGIFDNLSSGLKSNVDQR
                     ADFFEGDILNYEQIAAVLEEGWDAVIHLAAFKAAGESMIAPEKYSHNNISGSLNLIAA
                     CSKAKVKNFILSSTAAVYGEPEYLPVDEHHPTRPTNYYGYTKLAIEENLEWYSQLRDL
                     SYVSLRYFNAAGYDNEGRMLGLESNPANLIPVVMEVAMGIRPNLLIFGSDWDTPDKTA
                     IRDYVHVTDLATAHVLAVDYLMEGKGNLIVNLGSENGISVQQIVDTAREITGHPIPAE
                     YVERRKGDPAKLIASSKEARKTLGWKAVHSSVENIIASTWRVYLANQKRLES"
     gene            complement(4512..4584)
                     /locus_tag="PLF67_09030"
     tRNA            complement(4512..4584)
                     /locus_tag="PLF67_09030"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4549..4551),aa:Asn,seq:gtt)
BASE COUNT         1099 a         1258 c         1160 g         1081 t
ORIGIN      
        1 acctgcgggg atgatcgtca tactcccgcc tcgggcgatc gtcgtacctg cggggacgat
       61 cgtcatactc ccgtctcggg cgatcgtcgt acctgcgggg acgatcgtca tattccctcc
      121 tcgggcgatc gtcgtacctg cgggggcggt catcatcata ttcccgcctc gggcggtcgt
      181 cgtacctgcg gggatgatcg tcatattccc gcctcgggcg gtcgtcgtac ctgcggggcc
      241 gatcgtcata gtcccacctc ggccggtcgt cgtacctgcg ggggcgatcg tcatattccc
      301 gcctcgggcg gtcgtcgtac ctgcggggcc gatcgtcata gtctcgtctc gggcggtcgt
      361 caaaccttcg aggacgacta tcatctcgtc ttgatcgatc acttgaggaa gaacggcggc
      421 tttcacttcg ccgagcgcgt tctttcttca cttcggcctt ctgttcaggc tcatcatccc
      481 agaccaatgt ccatacgtca tcctcttcct cgcttaaaaa tgcctggtcc atgatttctt
      541 cgctccgggt cgcctctttg acctccgtct tgttcttttc ttcttctttg ctcatctctt
      601 cttcatccat ctgcgcttca ttatgcgctg tttccgtatt cttccggctg tttctgaata
      661 tcgccggtat cgtatgattc cgttcttttg tcagcaccca cgttcgggcg gtcccggatc
      721 gacgggtaaa tcccggggct aataaatctt gggtacgttc ttcgatcgtg taccctccca
      781 acgcgctttt atctagtgaa aaccctccca gactcgagga gacgatgatc gttcctccct
      841 tcttcaatag agcgctgagt gcatgaagcc agcgcacata atcacgcacg atatcgaagt
      901 cgtgctccat ttttcgtgaa ttggaaaagc ttggcggatc gaagatgatc aggtcataaa
      961 tccgtttctc gtccaaggca tccttaacat actcaaaagc gtcagcgttg atgcatcgat
     1021 aggtcggtcc gaagaacccg ttcgccgcaa ggttcgcttc ggcccacgca gtgtaggtcg
     1081 ctgacagatc gaccgagtcg acttgaaccg ccccaccccg ggccgcgtat acgctgaacg
     1141 agccggtata ggaaaagagg ttcagaaccc tcaatccata gctcatcgac cctaccaatg
     1201 ctcgggtcgg catatggtcc agaaacagac cggtatccac tcgtttcgtc agatcaaccg
     1261 tgaaaacgag gccttgctcc attacctcta cgagggtgga aacaggctct tgcacatcat
     1321 gctgctcgcg cccggtccgc ttccggcggt gatggtagac taccttctca atagggacat
     1381 acagcatccg accggcaaga tccatgacaa gatcccgctt gcgatcatcc aagccctctt
     1441 cactgtagtc ggtaattcga acccactgtg cccacaggtc gaccgtaacg ggaaactctt
     1501 ccatattccg gtcgtagacg cgcatgcaat tggtgtcagt tgattgaaac aaccgtcgca
     1561 tcgtaagggc attgttcttc agcaatttac caaaatcctt tgcttgataa tccatatgcc
     1621 ctcgctactc tttctgcaac gcagtgtata tgctactatg aaaaaaagct agtgaggcaa
     1681 ggacatacaa tggatgatga tgcccacaag ttcattcaag aacttgaaac aagacatgga
     1741 agcaaactct catggcgaac ctacgccaca tggtacgcca caacgaaggg agttgcccgc
     1801 gaattcggtg tattccttta caaagtaaat gattctttct tctttgagga ttttgagcgc
     1861 caaccggcgc tcttcggctt ttcgcttcgc ccccggaaga acagagagcc gtttgtgaag
     1921 tatgaagact cctttgcctg tacgggcgtt gaatccgttc gaaccgtttc cagaagcaat
     1981 gcattgaaga taatccggca gccgtcccat ccggtgaaag atgcctcctg gtttgacaaa
     2041 gccttccgtc aagtggtaga aatggtgctg ctgaaagacg ggacggttca cttctttgag
     2101 gtgatggatc gaaaggagtt cagaaggaga ttaaacgatg ggagcgttta aagcatacga
     2161 tattcgggga atctacaacg tggatttcaa caaagacacc gtgtacaaga tcggctactt
     2221 tttgcccaag cttctgaaga gcaaagcggt cctcgtcggt cgggatgtcc gaaccagcag
     2281 cgatgagatc tttgaagcat tgagcagggg aatcatggac agcggagcgg atgtctggaa
     2341 catcggtctg gcgaccaccc cgatggtgta ttttgcgacc gttcacttca aggcggatgc
     2401 ctccgtccag atcacagcca gccacaaccc cgccgagtac aatggactga aaatcagtcg
     2461 gacgaatgcc gtccccgtcg gttcggacag cggactgaag gaattggaag cgatggttct
     2521 taatgatgaa attgttccct cccgaaagaa aggaaagatc atcaggaaag aagcaaggaa
     2581 cgcatatatc gattttttga aagcgttcac tcccgacacc tccgacctca acctttcgat
     2641 cgacttttca cacggaatgg cgaatctcat cgtcaaagat ctcttggggg agaatcatca
     2701 ttacctctac gatcatcttg acggaacatt cccggctcat gagcccaatc ctctggaagt
     2761 cgaaaacacc tacgatcttc gcgatgcggt aattgccaac aaaagcgaca tcggaatcat
     2821 ctacgacggc gatgccgacc gcgtcatgtt ccttgatgag aacggccgct ttctccaacc
     2881 cgattacatc accagtctct tgggcctcta ttacctcaaa aaggaaaaag gccgagttct
     2941 ggttgatatt cgtaccagcc gttcaacggt tgaatacctt gaaagcctcg gtgccgaggt
     3001 tcacgtgtgg aaagtcggtc atgcgttcgc gaagaacaag cttcgggagc ttggagcgat
     3061 cttcggcggc gagcttgccg gacactacta tttcagagac ttcttcaact gcgacagcgg
     3121 cattcttgct tccctcctcg tccttcaggc tgtgaagatg ctcaaaactg aaggtcagac
     3181 aatcggctcc ttcatcgatt cgatcgtcag gtgggcaaac agcggagaag tgaactacac
     3241 cctcaatgaa aaggatgaag cgatgaccgc tctgtatgag cgatatgtca aaaacgattc
     3301 ccccaccctt gtcatggatt tcgacggcta tcgggttgaa ttcaaggatt ggtggttcaa
     3361 cgtgcgtaaa tccaacaccg aaccctactt gcggttggtt gttgaggctg ctacccaagc
     3421 tttgcttgat gaaaaactgg ccgatctgag cacaatcatt acccgctttt cataaggaga
     3481 tgaagcgatg aagatattac tagtcggagg ggccgggtac atcggtagcc atgtcgctct
     3541 ccaattcctc gaacgaggag accaagtcgg catttttgac aacctttcaa gcggcttgaa
     3601 aagcaatgtt gatcagcgtg ccgacttctt tgaaggggat atcttaaatt atgagcaaat
     3661 cgccgctgtc ctcgaagagg gatgggatgc ggtgatccac cttgcagcat tcaaagccgc
     3721 aggagagtcg atgatagcgc cggaaaaata ttcccacaac aacatctcgg gatccttgaa
     3781 cctcatcgcc gcctgctcga aagccaaggt gaagaatttc atcctctcct ccaccgccgc
     3841 cgtctacggc gagccggagt atctgccggt ggatgagcat catccgaccc gtccgacaaa
     3901 ctactatgga tacaccaagc tcgccattga agagaacctg gagtggtatt cacagcttcg
     3961 ggatctttcc tacgtctccc tgcgctactt caatgcggct gggtatgaca atgagggtcg
     4021 aatgctcggt cttgaatcaa acccggcgaa cctgattccc gtggtcatgg aagtggcgat
     4081 gggaatcagg cccaatctct taatcttcgg cagcgactgg gacaccccgg acaagaccgc
     4141 aatccgtgat tacgtgcacg tcaccgatct ggcgactgcc cacgtcctgg cggtcgatta
     4201 tttgatggaa ggcaagggaa acctcatcgt gaacctcggc tccgaaaacg gcatcagtgt
     4261 ccaacagatc gttgacaccg cccgcgagat caccggtcac ccgattcccg ccgaatatgt
     4321 ggagcgaagg aagggcgatc cggcaaagct catcgcctca agcaaggaag caaggaaaac
     4381 cctcggttgg aaagccgtac actcaagcgt tgaaaacatc atcgcttcaa cgtggcgggt
     4441 gtatttggcg aatcagaaac gactggagag ctagaaacga gaagagacaa ccccgaagga
     4501 ttgtctcatc gctcgcccag agggatttga acccccgaca gggtggttaa cagccacctg
     4561 ctctaccgac tgagctatag gcgaatgtct gttgaacg
//
LOCUS       DAOSLI010000097         4556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_16793_length_4556_cov_16.103088, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000097 DAOSLI010000000
VERSION     DAOSLI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16793_length_4556_cov_16.103088"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..653)
                     /locus_tag="PLF67_09035"
     CDS             complement(156..653)
                     /locus_tag="PLF67_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOE90193.1"
                     /translation="MDQEVDEELAAKLFRESQKQGCYAQGLSYLARSEHTTFHLVQKL
                     TRKGYPKEIIDDAVSRLTEEGALSDLRYARLFIERRQRKNPEGRLRLAQRLADRGVRN
                     DAALQALDELFTEEHIAAYVARAVNRAKTNHPDTDVRNLLRAWGFSSYEIRTGLEQAG
                     DDELP"
     gene            complement(769..1182)
                     /gene="rpsI"
                     /locus_tag="PLF67_09040"
     CDS             complement(769..1182)
                     /gene="rpsI"
                     /locus_tag="PLF67_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOE90194.1"
                     /translation="MAKKEANKAVNLGHGVGRRKTAVARVYLREGEGKIMINGRDVDS
                     YFGNPMLTFIVKQPLVTTETDGKYDILINVKGGGVSGQAGACRHGIARALAIHDEAFR
                     PALRAAGFMTRDSRMVERKKYGQPGARRKFQFSKR"
     gene            complement(1197..1625)
                     /gene="rplM"
                     /locus_tag="PLF67_09045"
     CDS             complement(1197..1625)
                     /gene="rplM"
                     /locus_tag="PLF67_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOE90195.1"
                     /translation="MKTIFVKPLTIQKKWYLIDAEGKELGRVAVAAARILRGKNKAEY
                     APLHDLGDYVIIINAEKAILTGNKYNDKMYYRHSGFPGGLRKTSYAEMIKRKPTFPME
                     HAVKGMLPKGPLGRKLFTNMRVYAGAEHKQSAQQPIPVEI"
     gene            complement(1983..4199)
                     /locus_tag="PLF67_09050"
     CDS             complement(1983..4199)
                     /locus_tag="PLF67_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE90196.1"
                     /translation="MAPIETYLKQLNEPQREAVLENSRPLLMLAGAGSGKTRVITTKI
                     AYAIEALKIPPYRILAVTFTNKAATEMKERVEALLPDNPDVKDCNIRTFHSFGAWLLR
                     RFGSEIGLENSFTIYDDADSLSLLASIYPSYKKRELEPVMKRISYAKDRMLSPGEAMK
                     GFQADLLFKQMYERYEEKLRAVGNLDFADLIGRSIELLRDAPHVQQWVHSRFEMIMVD
                     EYQDSNTAQFLLLKLLVGPETFVGVVGDDDQSIYRFRGAEVKNILTFASSFPKARTIK
                     LEQNYRSTTQILDVANSIISKNADRHDKNLWSARKGGRKPKLFYVHDEEDEARRIVTL
                     LREDHNYNGSAVLYRTNAQSVPFETTFKRYGIPYKVVGALQFYEREEVKDALALLYLL
                     TNPKDEVSFRRIINKPARGIGPGSVDKIIGFALQTDGDLLRAAALAVQNRTLSPKAAQ
                     GAELAVRLIDEGRKLIVDGNLVDGVTFLIRESGLYEHYQEQDYLNTTAKVGNLEALVN
                     ALSAYESSFDGLVAFLEQLTLDRTTLGTDDPRDKDGVTLITMHNTKGLEFDRVFVTGL
                     EEGLFPGRQNEGDEDIQEERRIFYVAVTRARNELYLFSSRVRKIWGRTTFQHPSRFIA
                     DIDPALIRVEGSPSLGGYNSFTRRESSGWGSSGSSMIRQGFGPGAKQFEVRHEQQGDA
                     PLFPVGQKVYSDAYGEGEVVALRGEGEREVIDVVFATGKKASYLSRFAALDRIGGE"
     gene            complement(4186..>4556)
                     /locus_tag="PLF67_09055"
     CDS             complement(4186..>4556)
                     /locus_tag="PLF67_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOE90197.1"
                     /translation="SFVYSLEEGTKAAELTNERAHRKLASKARRFQQELEALQLSITQ
                     ARLKTFVGRKIDVLIEEHVEGEELYIGRTYAQAPEVDGLTVVLARDLVPGTVVRCGIT
                     RVNGIDFEAVPLYGGYDGSH"
BASE COUNT          950 a         1309 c         1234 g         1063 t
ORIGIN      
        1 caaagagaag ggttcccgga agcgccaggc tcaggtacat ccccacatca ccgattcggt
       61 cgaggtcttc ggaatattcg aagcggaacg cggagagtcc attcgagaac acaacgacga
      121 gcgtaaacag aacaaggatt tttctggtca cggctttacg gtaattcatc atcacctgcc
      181 tgttcaagtc ccgttcggat ctcataggag gaaaaccccc atgcgcggag aagattccgc
      241 acatccgtat ccggatggtt ggttttggcc cgattcaccg ctctggcgac ataggcggcg
      301 atatgctctt cggtgaagag ttcatccaat gcctgcaggg cggcatcgtt cctgactccc
      361 cgatcggcaa gacgctgagc cagacgcaag cggccttcgg gattcttgcg ctgcctgcgt
      421 tcgatgaaca gccgggcgta gcgcagatcg ctgagcgccc cctcctcggt gagtcgggaa
      481 acggcatcat caatgatctc tttcgggtat cctttccggg tcagcttctg aacgaggtga
      541 aacgtggtat gctcgcttcg ggcgaggtag gaaagacctt gggcgtagca cccctgtttt
      601 tgactttccc gaaagagctt ggcggcaagc tcttcatcaa cttcctgatc caccttcaag
      661 ccatatgtac ggaacaagtc cgaagagata aaaaaagggg aaccctgttg agggaggagt
      721 tgataccctc ccttcttggg ctccctgacg atccgtgcgt aaaacacctt atcgtttgga
      781 gaactggaac tttcggcggg cgccgggctg accgtatttt ttccgctcaa ccattcgcga
      841 gtcacgggtc ataaacccgg cggctcgaag ggcgggcctg aaggcttcat cgtggatggc
      901 gagggcgcgg gcgattccat ggcggcatgc accggcttgg ccggagactc caccgccctt
      961 gacgttgatg aggatatcgt acttgccatc cgtctcggtg gttaccaacg gttgtttgac
     1021 gatgaacgta agcatcggat tgccgaaata gctgtcgaca tcccgaccgt tgatcatgat
     1081 tttgccttcg ccttcacgaa gatagacgcg agcgaccgca gtcttgcggc gaccaacgcc
     1141 atgcccaagg ttcactgctt tattagcttc tttctttgcc attattcgct cctaccttag
     1201 atttcgaccg gaatcggttg ctgggcgctt tgcttatgct cggcaccggc atagacacgc
     1261 atgttggtga acaacttgcg gccaagcgga cccttgggta gcatcccttt gactgcgtgc
     1321 tccatcggga acgtcggctt gcgcttgatc atctcggcat agctggtttt gcgcagtccg
     1381 ccggggaagc ccgagtggcg gtagtacatc ttgtcgttgt acttgtttcc ggtaagaatc
     1441 gctttttcag cgttgatgat gatcacataa tcaccaaggt cgtggagggg ggcgtactcg
     1501 gctttgtttt tcccccggag aatgcgtgcc gcggctaccg ccactctgcc aagctccttt
     1561 ccctctgcat caatcagata ccatttcttc tgaatcgtca gcggtttcac aaaaatagtc
     1621 ttcatccatt catcctgctc tgcaagggtt tccttgcctg taattaatga tgcaggttcc
     1681 tgcatcgtca tggcctttcc ggaagaagaa gaaaccggcc ggctgatgag caaatcagca
     1741 aaggtctccc cgctcgccga tcaccgcatc cggccaaggc ttcggtcctg gacaactccc
     1801 cgggaaaggc aggttaggaa ggagcgcacc atcgtgctcc gacaaggtcc aaagcatcct
     1861 tgaaaagaat cgaatatgat cgggttcttc acagcgtaac gccatatctg gtaaaaaatc
     1921 cacgccccga agggcggaca acggtaagca ttgtataatc ttccgcccaa tcgtgtctag
     1981 ccttattcac caccaatacg atcaagggcg gcaaagcggc tcaggtagct ggctttcttc
     2041 ccggtggcga agacgacgtc gatcacctcc cgttcccctt caccccgaag agcgaccact
     2101 tccccttccc catatgcatc gctgtacacc ttctggccga cgggaaacag gggggcatcc
     2161 ccctgttgct catgcctcac ctcgaactgt ttcgcgccgg ggccgaatcc ttgacggatc
     2221 atgctcgaac cgctgctccc ccatccggac gattccctcc tcgtaaacga gttgtacccc
     2281 cccaaagaag gagatccttc aacccggatc agagcgggat cgatatcggc gatgaagcgg
     2341 cttggatgtt ggaacgtggt gcgtccccag atcttccgaa ccctcgagga gaagagatag
     2401 agctcgttcc gagcccgggt gaccgcaacg tagaagattc gccgctcctc ctggatatcc
     2461 tcgtcccctt cattttgacg gccgggaaag agcccttcct ccaagccggt gacgaacacc
     2521 cgatcgaact ccaaaccctt ggtgttgtgc atcgtaatga gggtcacccc atccttgtct
     2581 cgaggatcgt ccgttccaag ggtggttcga tcgagagtca gctgctccag gaatgcgaca
     2641 agtccgtcaa acgaggactc atacgccgaa agggcgttta ccagtgcttc gaggttgccg
     2701 accttcgctg tggtgttgag gtagtcctgt tcctgataat gctcgtagag acccgactcg
     2761 cggatcagga acgtcacccc gtccaccaga tttccatcga caatgagctt tcggccttca
     2821 tcgatgaggc gaaccgcgag ctcggctccc tgggcggctt tcggggagag cgtccggttt
     2881 tggaccgcca aggcggcagc ccgcagaaga tccccatccg tctgcaaggc gaagccgatg
     2941 atcttgtcga cactgccggg tccgatcccc cgagcgggtt tgttgatgat tcttcggaag
     3001 gagacttcgt ccttcgggtt cgtcaacagg tatagaagag ccaaggcgtc cttcacttct
     3061 tcccgttcgt agaattgaag agctcccacc actttgtagg gaatcccata tcgtttaaac
     3121 gtcgtttcaa aaggaacgct ctgggcgttg gttcgataga gcaccgcgct gccgttgtag
     3181 ttgtggtctt cccgaagaag cgtgacgatc ctgcgagcct catcctcttc atcgtgcaca
     3241 tagaagagct tgggcttccg tccacccttc ctggcgctcc aaaggttctt atcgtgccga
     3301 tcggcattct tggagatgat gctgttggcc acatccaaaa tctgagtggt cgaacggtag
     3361 ttctgctcca gtttgatcgt tcttgctttt ggaaacgagg aggcgaaggt aaggatgttc
     3421 ttcacttccg ccccccggaa ccgatagatc gactggtcgt catcgccgac gaccccgacg
     3481 aaggtctcgg gaccgacaag gagcttcaac agaagaaact gggcggtgtt ggagtcctga
     3541 tactcgtcga ccatgatcat ctcgaagcgg gagtggaccc actgttgcac atggggcgca
     3601 tctcggagca actcgatcga gcgcccgatc agatcggcga aatcaaggtt tcccaccgct
     3661 cggagctttt cttcataccg ttcgtacatc tgcttgaaga gcagatcggc ttgaaagccc
     3721 ttcatcgctt caccggggga gagcatccgg tccttggcgt agctgattcg cttcatgacc
     3781 ggttcaagct cgcgtttttt gtaggaagga tagattgagg cgagcaatga aagcgaatcg
     3841 gcatcatcat aaatcgtgaa actattctcc agcccgatct cgcttccgaa tcttcttaag
     3901 agccatgcac cgaaggagtg aaacgtccgg atattgcaat ccttcacatc cgggttgtcc
     3961 gggagcagcg cttcgacccg ctctttcatc tcggttgccg ctttgttggt gaacgtaacg
     4021 gcgagaatcc tgtagggggg gatcttcaac gcctcgatcg cataagcgat cttggtggtg
     4081 atcacccggg tctttccgct tcccgccccg gccagcatca aaagcggccg gctgttttca
     4141 agcaccgcct cccgttgggg ttcgttcagt tgcttcaagt atgtttcaat gggagccatc
     4201 atatcctcca tagaggggaa ccgcctcgaa gtcgatgccg tttacgcggg ttatcccgca
     4261 gcggaccacc gttcccggta ccaaatctcg ggccaacacg acggtcaggc cgtccacctc
     4321 gggcgcctgg gcgtaggttc ggccgatata gagctcctcg ccctcgacat gctcttcgat
     4381 gagcacgtcg atctttcggc cgacaaaggt cttcaagcgc gcttgggtga tggagagctg
     4441 aagtgcttca agctcctgtt gaaacctacg cgctttggaa gcgagtttcc tgtgggctcg
     4501 ttcattcgtc aattcggcgg ctttcgtgcc ctcctccagc gaataaacga acgagc
//
LOCUS       DAOSLI010000098         4549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_16822_length_4549_cov_13.874722, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000098 DAOSLI010000000
VERSION     DAOSLI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16822_length_4549_cov_13.874722"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLF67_09060"
     CDS             <1..386
                     /locus_tag="PLF67_09060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE90198.1"
                     /translation="FVEGQGVPEAIDFDGQDGSSGHILLFDTNIPVGTTRIRKTDKGM
                     KLERIAVLPAYRKKGYGGLLVKAALSKVTGTIYINAQVASLGFYEHLGFVQEDKTTFL
                     EAGIPHTVMLWPHGQSEALCPILEP"
     gene            complement(439..1503)
                     /locus_tag="PLF67_09065"
     CDS             complement(439..1503)
                     /locus_tag="PLF67_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE90199.1"
                     /translation="MKKRIAKISVVLAALLVLVMLPLAAGGGKEEKATDPTAMEKNLV
                     LYSSMTENDLSNLVRLFNKQYPDVKIEIVNGSAGELTSRIRAERNNPQGDLMWGGLSS
                     SDGMVHGDLFEHWLTEYEPDIMPDYRSNNGFYSLDHLSTAVFCVNVELEKELGLNIRG
                     YKDLLDPRLKGKIVMPDPNSSSSGWNNVSNMMAVFGHGTPEVWDYITKFMNNQVVFST
                     ASSVAFKSVDTGEYVVGLTYEDGASTLLKAGSNRIRMVYPEDGASASAFGCALIAGAK
                     NPNAAKAMINYLMSAEGQSELGMALETLRFTNEKAVYETPYLPASSEIKWVTRDNDWL
                     IANKKAVLEQWNKIYANVKK"
     gene            complement(1547..3199)
                     /locus_tag="PLF67_09070"
     CDS             complement(1547..3199)
                     /locus_tag="PLF67_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE90200.1"
                     /translation="MFAKKRFDVWSGVTLGFVILFGTFLVYPMFGILRQSVLNEGGEF
                     TLQHFVKFFSQKYYSMTIWNSLKVTISITLVTLLIGIPFSYFYTFYHIKGSKVIFVVS
                     ILCAMSAPFIGAYAWILLLGRGGVITQFLKNQLNINIGSIYGFKGILIVQSLKLYPLV
                     FLYMNGAFRNIDSYLLEASASLGCVGIKRFFRVVLNLSMPTILAASLLVFMQAFADFG
                     TPILIGEGYRTFPVEIYNQYLGEVGTDYNFASAISVIAIIMTALVFFLQKYATSKYNF
                     SMNATRPVEKKKAKGIRGALMYLYCYGILAIAFMPQIYVSYLSFRNSDSVVFLEGYSL
                     ASYRMAVDKLLFRSIKNTLVLGFGALAIIIVFSVLIAYLVVRRPSWINNVIDTLSMLP
                     YIMPGAIIGIALVVAFGQPPLVLSGTMAIMIISFVIRRIPYSIRSTNAILMQIPISVE
                     EAAISLGASKLKTFVVITVPMMVNGIISGAILSWVAIVTELSSSIILYNNRNITLTMS
                     TYVAISRGSYGLACAFSAILTLFTTVSLLLYLVISKTEDIRL"
     gene            complement(3212..4324)
                     /locus_tag="PLF67_09075"
     CDS             complement(3212..4324)
                     /locus_tag="PLF67_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE90201.1"
                     /translation="MSEHITIVHALKKYGENVIIPDLSMNIKEGEFFTLLGPSGCGKT
                     TLLRMIAGFNTIEGGDFYFSDQRINDLDPAKRNIGMVFQNYAIFPHLTVRKNVEFGLK
                     SRKYSREEITKQAEKYMRLMQIQQFAERMPAQLSGGQQQRVALARALCIEPSVLLMDE
                     PLSNLDAKLRVEMRSVIKDTQRDVGITTIYVTHDQEEAMAISDRIAVMDEGVIQHIGT
                     PKDIYQRPANLFVATFIGRTNIVDAELVVKEGKTFLKVGSFDVEMTNVEKKHAKNQKV
                     IVSIRPEEYKILPKEEKGIKAEVKESVFLGLNTHYFVRTERGNDVEVVQESTIDSIIP
                     PGETISLGLNVSKINLFTSDGSANILKGVKNDLANM"
BASE COUNT         1095 a         1207 c         1150 g         1097 t
ORIGIN      
        1 tgttcgtcga aggccagggc gttcccgaag cgatcgactt cgacgggcag gacggttcat
       61 ccggccacat cctgctgttt gatacaaaca tacccgtcgg aacgaccagg atccgcaaga
      121 ccgacaaagg aatgaagctc gaacgaatcg cagtcctgcc cgcctaccgc aaaaaaggct
      181 acggcgggct cctcgtgaaa gcggccttga gcaaagtaac cggaacgatt tacatcaacg
      241 cccaggtggc aagcctcggc ttctatgagc acctcggctt cgtccaggaa gacaaaacga
      301 ccttccttga agccggcatc ccccacacgg tcatgctctg gccccatggc caaagtgaag
      361 cactctgtcc cattttggag ccataataaa accccgaaga aaacctccgg ggtgcgtaga
      421 gagcaggaaa tctaccgatt atttctttac attcgcatag atcttgttcc attgctccaa
      481 aacggctttc ttgttcgcga tcaaccagtc gttgtcacgg gttacccatt tgatctccga
      541 gcttgccggc aggtacgggg tctcgtagac ggccttttcg ttcgtgaacc gaagggtttc
      601 aagagccatt ccgagctccg attgtccttc cgcgctcatc aggtaattga tcatcgcttt
      661 cgcagcgttg gggtttttgg ctcccgcgat caaggcgcaa ccgaatgccg aggcggaggc
      721 accgtcttcg ggatatacca tcctgatgcg attggaaccc gccttcaaga gggtcgaagc
      781 gccatcctca taggtcaatc cgacaacata ctcgccggta tcaacgctct tgaacgcaac
      841 ggagcttgcg gtggagaaca ccacttggtt gttcatgaac ttggtgatgt aatcccaaac
      901 ctcgggcgtg ccgtggccga agaccgccat catgttgctt acgttgttcc aacctgaaga
      961 ggatgagttc ggatcgggca tgacgatctt gcccttcagc cgaggatcga gcagatcttt
     1021 atatccgcgg atattgagac caagctcttt ttccagctcc acgttgacgc agaataccgc
     1081 ggtggagagg tggtcaaggc tgtagaaacc gttgttggag cggtagtccg gcatgatatc
     1141 cggctcatat tccgtcagcc aatgctcgaa gagatcgccg tgaaccattc cgtcgctgct
     1201 ggagagtcca ccccacatca agtctccttg agggttgttg cgctctgccc ggattctgct
     1261 ggtgagctcg ccggccgaac cgttgacgat ttcgatcttc acatccggat attgtttgtt
     1321 gaacaagcga accagattgg aaagatcgtt ttccgtcatg ctggaataga gaacaaggtt
     1381 cttctccatt gcagtgggat ccgttgcttt ttcttctttt ccgccacctg cggcaagcgg
     1441 caacatcact aaaacgagaa gtgctgccag cacgacgctt attttcgcaa tacgtttctt
     1501 catcgtattc cctccttaaa attatcagcc ttacagaccg atcaggttac agtcgaatat
     1561 cttcggtttt gctgatcact aaatacagaa gcagcgaaac cgtcgtaaag agcgtcagga
     1621 tcgcgctgaa agcacaggcc agaccatagc ttcctctgga gatcgcgaca tacgtggaca
     1681 tcgtgagcgt gatgttcctg ttgttgtaca ggatgatcga gcttgaaagc tcggtgacga
     1741 tcgccaccca gctgagaatg gcgccgctga tgattccatt gaccatcatc ggcacggtta
     1801 tcaccacgaa ggtcttgagc ttggacgcac cgaggcttat cgcagcctct tcaacactta
     1861 tcggaatctg catgagaatc gcattggtcg atcgtatcga atacggtatg cgccggatca
     1921 cgaacgagat gatcatgatc gccatcgttc ccgaaagaac caacggcggc tggccgaatg
     1981 cgacgaccag ggcgatgccg atgatcgctc cgggcatgat gtacggcagc atcgacagcg
     2041 tgtcgatgac gttgtttatc cagctgggcc tgcgcacgac tagataggcg atgagcactg
     2101 aaaagacgat gatgatcgcc agagctccga accccaggac aagggtgttc ttgatggaac
     2161 ggaacagaag cttgtctacc gccatccggt agcttgccag ggaatagcct tccaggaaca
     2221 cgaccgaatc gctgtttcgg aacgaaaggt agctgacata gatttgaggc atgaacgcta
     2281 ttgccaagat tccatagcag tagaggtaca tcagcgcccc tcgaatgccc tttgccttct
     2341 ttttctcaac cggccgggtt gcattcatcg aaaagttgta cttgctcgtt gcatatttct
     2401 ggaggaaaaa caccaaagcg gtcatgatga tcgcgatgac gctgattgct gaagcgaagt
     2461 tgtaatcggt tccgacttct ccgagatact ggttgtagat ttcgaccggg aaggttctgt
     2521 acccttctcc gatgagaatc ggcgttccga agtcggcaaa cgcctgcatg aacaccagca
     2581 aggaagctgc aaggattgtc ggcatcgaaa ggttgagcac caccctgaag aagcgcttga
     2641 tgccgacgca tccgaggctc gcggaggctt caagcagata gctgtcgata ttcctgaacg
     2701 ccccattcat atagaggaac accaacggat agagcttgag cgattgaacg atcaggattc
     2761 ccttgaaccc gtagatcgaa ccgatgttga tgttgagctg gtttttcaaa aactgggtga
     2821 tcaccccgcc ccggcccaac agcagaatcc aggcgtatgc cccgatgaac ggagcgctca
     2881 tcgcacacag gatgctgacg acgaagatga ccttcgatcc cttgatgtga tagaaggtat
     2941 agaagtacga gaagggtatt cctatcagaa gggttacgag ggttatcgaa atggtgactt
     3001 tgaggctgtt ccaaatcgtc atggaatagt atttctgcga gaagaacttc acaaagtgct
     3061 gaagcgtgaa ctccccgccc tcattcaaaa ccgattggcg aaggatgccg aacatcgggt
     3121 agacgaggaa agttccgaag agaatgacga accccagcgt cactccgctc catacatcga
     3181 aacgtttttt cgcaaacatc tcttccctcc cctacatgtt ggccaaatcg tttttcacac
     3241 ccttgaggat gttggccgac ccgtcagaag tgaacaggtt gatttttgaa acattcaacc
     3301 cgagactgat cgtctcgccc gggggaatga tggaatcgat cgtcgattcc tgaaccactt
     3361 cgacatcgtt gcctcgttcg gtccggacga agtagtgggt attgagccca aggaagacgc
     3421 tctccttcac ctcggccttg attccctttt cttctttcgg gaggatcttg tactcttcgg
     3481 gtcggatgga aacgatcacc ttctggttct tcgcatgctt cttttcaacg ttggtcatct
     3541 cgacatcgaa ggagccgacc ttgaggaacg tctttccctc tttgacgacc agttcggcat
     3601 ccacgatgtt cgttcgaccg atgaaggttg caacaaacag attggccggg cgttgataga
     3661 tatccttcgg agtgccgatg tgctgtatca cgccctcatc catgactgcg atgcggtcgc
     3721 tgatcgccat cgcctcctcc tgatcgtgcg tcacgtagat cgtggtgatc cccacgtccc
     3781 gctgcgtgtc cttgatgacc gaccgcatct caacccgaag ctttgcatcc aagttggaaa
     3841 gaggctcatc catcaacagg acggaaggct cgatgcaaag ggctcgggca agggcgaccc
     3901 gctgttgctg tcctccggac agctgagcgg gcatccgttc ggcaaactgt tggatctgca
     3961 tcaggcgcat atacttttcc gcctgttttg taatctcctc tctcgaatac tttcggcttt
     4021 tcaacccgaa ttcaacgttt tttcggacag taaggtgggg aaagatcgca tagttctgga
     4081 acaccatgcc gatgttgcgc ttggccggat caaggtcgtt gatccgttgg tcgctgaaat
     4141 aaaaatcacc gccttcgatc gtgttgaatc cggcaatcat ccgcagcaag gtggttttcc
     4201 cacacccgga aggaccaagc agggtaaaga attcaccttc cttgatgttc atgctcaagt
     4261 ccggaatgat gacgttttct ccatactttt tcagggcatg gacaatggtg atatgttcgc
     4321 tcacaacaag acactccttg caattaactt acaacctgaa ttaaaattaa cacgcaccaa
     4381 ataggtagtc aattaaagga aattgtttaa aaaccctcaa atttgttgtc taaaagggtt
     4441 tttgtaccac cccaaaaaaa tcccgttctg tgcaccccct tggacacttc cgcttttcct
     4501 gctacagtag actcggagga ttttgatgaa gattcaacat tcttgtcgt
//
LOCUS       DAOSLI010000099         4505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_17024_length_4505_cov_28.289888, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000099 DAOSLI010000000
VERSION     DAOSLI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17024_length_4505_cov_28.289888"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1393
                     /locus_tag="PLF67_09080"
     CDS             122..1393
                     /locus_tag="PLF67_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE90202.1"
                     /translation="MKKIVIFSLIALLSIGSVFAAGTKESVPAQKTVTVFGAFVDEEA
                     RRFEEAIRPFEERTGIKVVYEGSKDFETLINVRVEGGNAPDIAGLPQPGLMANFASQG
                     KLVPLWPSALALLEKNYAPVWKDLGSYKGVAYGVFHRVNAKSFVWYPKKAWDAAGYQV
                     PKTWDELKALMDRMVRNGHTPWAVGIESGGATGWVGTDWIEDIMLRTAGPDLYDKWVG
                     NEIPFNAPEVKKAFEILGDIWMNPKYVYGGRNTILTEGFGDSQRPLFDNPPKAWMHRQ
                     ANFITGFFPEHIQANLEEEVGVFPLPAIDPKWGIPVLGGGDQFVVFNDRPEVREFMEY
                     LQTWESAESWAKAGGALFPYLNQNLDAYPNAIERSLAEALVNAKVFRFDASDLMPASV
                     GAGSFWEGIVDWVNGKSLDATLADIQKSWPK"
     gene            1448..2515
                     /locus_tag="PLF67_09085"
     CDS             1448..2515
                     /locus_tag="PLF67_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE90203.1"
                     /translation="MNTKAKTIVLSILFPLATLAILATGFVFLRDTKGPQVLTAVIAI
                     IWGVGGAALLFYTVDWLVNRFKPEIARKLQPFVFVGPALLILLWYLVLPTIRSLYLSF
                     FDASSRNFVGLENYVFAFTDGKMLESFRNNLMWIILGTGGSVGFGLLIALLVERSKAE
                     SVFKSIIFMPMAISFVGAGIIWRFVYAYKPAGEDQIGVLNALVTFFGGEPQAWFTIAP
                     WNNIFLIIILIWLQSGYAMVIISSALKGVPAEIIEAGRIDGAGELRVIRSIIIPYIYS
                     TLVTVSTTIVIISLKIFDIVFTMTNGNYGTEVIASVQYKQMFRFYHYGRGSAIAIVLV
                     LAVTPVMWYNLRQFAKQEVFK"
     gene            2512..3402
                     /locus_tag="PLF67_09090"
     CDS             2512..3402
                     /locus_tag="PLF67_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE90204.1"
                     /translation="MNNRKASIAINTALLILVVIWFIPILGLFISSFRPRDYVLTTGW
                     WTAFTKNRIFTLDNYRQVLGGTKYTFVDALGNTVRSSGDNLSQAFINSFTVTIPSVII
                     PILIAAAAAYGFAWMVFPGRKFFFTSVVALLVVPLQIALIPILRDYQKIGLTGSYLGI
                     WLAHTGFGLPLSIYLLYNYISTIPRSIFESAYLDGARQIVVFARLVLPLSIPAIASLA
                     IFQFLWVWNDLLVALVFLGGTQDVAVLTQRLATMIGSRGQDWHLLTAGAFVSMVVPLA
                     VFFSLQRYFVEGLMAGSVKG"
     gene            3406..>4505
                     /locus_tag="PLF67_09095"
     CDS             3406..>4505
                     /locus_tag="PLF67_09095"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90205.1"
                     /translation="MNTNWLLTREGPNRNGHTESLYSLGNGRLGFRGFPLWRDPLFQG
                     GVFLNGLYDTRPIRYEEDVYGSAKIAQHIVPLPDSRFIAIRINDEPIRPINERKTLDF
                     KTGILTHQVETKEAVIEYETFLSMTRTSLGVVTLNIEQTGNNRISILSGIAQDEVSTS
                     NTFDPRVANHIPKLRTINASHTDDGFTAEFETENSNLAVHMTTSHRCDIQPKETTYNN
                     LPALLYETTEKNIDLTKFFSYETERKPLSHPSYETLKEEQVAYYERFWQKSDVRIQDN
                     PTLQLALRFNIFQLNQSTGHALAAKGLTGVGYEGHSFWDTEIYALPFFTHTNPEKARD
                     LLLYRIGMLPYAKKRARELSQKGALFPWRTIYG"
BASE COUNT          933 a         1450 c         1216 g          906 t
ORIGIN      
        1 atgttgacag atggaaaaag gtgtgcaaga ctatatatgg aagcgcttcc acgacgcttc
       61 ttcattctca taattcgtta ccaagtcttg gtgtaggaac caagcataag gaggaatatt
      121 catgaaaaag attgtgatct tctcactgat cgctctgctt tcaatcggct ccgtcttcgc
      181 agccggtacc aaggagagcg tcccggcgca aaagaccgta acggtcttcg gcgcctttgt
      241 cgacgaagag gctcgccgct tcgaggaagc gatcaggccc tttgaagaaa gaaccgggat
      301 caaggtcgtc tacgaaggct ccaaggactt cgagaccctg atcaacgtcc gggtcgaagg
      361 cggaaacgcc cccgacatcg ccggccttcc ccagcccggc ctcatggcga acttcgcctc
      421 ccaaggcaag ctcgttcccc tctggccgag cgccctcgcc ctcttggaaa agaactatgc
      481 accggtctgg aaggacctgg gctcctacaa gggggtcgcc tacggcgtct tccaccgggt
      541 gaacgccaag agcttcgtgt ggtatcccaa gaaagcgtgg gatgccgccg gctatcaagt
      601 gccgaagacc tgggatgagc tgaaggccct gatggacagg atggtccgaa acggccatac
      661 cccgtgggcg gtcggaatcg agtccggcgg agcgaccgga tgggtcggaa cggactggat
      721 cgaggacatc atgcttcgca ccgccggtcc ggatctctat gacaagtggg tcggcaacga
      781 gatcccgttc aacgctcccg aagtcaagaa ggccttcgaa atcctcggcg acatctggat
      841 gaaccccaaa tacgtctacg gcggccgcaa cacgatcctc accgaggggt tcggcgattc
      901 ccagcgcccg ctcttcgaca atccgccgaa ggcatggatg caccgccagg cgaacttcat
      961 caccgggttc ttccctgagc acattcaggc caacctggag gaagaggtcg gagtcttccc
     1021 gctgccggcg atcgatccca aatggggcat cccggtgctc ggcggcggcg accagttcgt
     1081 cgtcttcaac gaccggccgg aagtccgcga gttcatggag tacctgcaga cctgggaaag
     1141 cgccgaatcc tgggcgaaag ccggtggagc cctcttcccc tacctcaacc agaatcttga
     1201 cgcctatccc aacgcgatcg aacgctccct ggccgaagcg ctcgtgaacg cgaaggtctt
     1261 ccgcttcgac gcatccgacc tgatgccggc ttccgtcggc gccggatcct tctgggaagg
     1321 aatcgtcgac tgggtcaacg gcaagagcct cgatgccacc cttgccgaca tccagaagtc
     1381 ctggccgaag taagagcttc ttcgtatggt gccggcgtcg ccggcaccac attccttgga
     1441 ggattcaatg aacacgaaag ctaaaacgat cgtcctgtcg attctcttcc cgttggcgac
     1501 tctggcgatt ctcgccacgg gattcgtctt cctgagggac acgaagggcc cgcaagttct
     1561 caccgcggtg atcgcgatca tctggggagt cggaggcgcc gccctcctct tctacaccgt
     1621 cgattggctc gtcaaccgct tcaagccgga aatcgcccgg aagctccagc cgttcgtgtt
     1681 cgtcggaccc gccctgctca ttctcctctg gtatctcgtt ctgcccacca tccgctccct
     1741 gtacttgagc ttcttcgatg cgtcgagcag gaacttcgtg ggacttgaaa actatgtctt
     1801 cgcattcacc gacgggaaga tgctggaatc cttcaggaac aacctcatgt ggatcatcct
     1861 cggaaccggc ggctcggtcg ggttcgggct cctcatcgcc ctgctggtcg agcgcagcaa
     1921 agcggaaagc gtcttcaaat cgatcatctt catgccgatg gcgatcagct tcgtcggagc
     1981 cggcatcatc tggcgcttcg tctatgcgta caagccggcg ggagaggatc agatcggtgt
     2041 tctgaatgcg ctcgtcacgt tcttcggagg ggagccgcag gcgtggttca ccatcgctcc
     2101 atggaacaac atctttttga tcatcatcct catctggctg cagagcggct atgcgatggt
     2161 gatcatctca tccgccctca agggcgttcc cgccgagatc atcgaagcgg ggcgcatcga
     2221 cggcgcgggg gagctcaggg tcatccgctc gatcatcatt ccctacatct attcgaccct
     2281 ggtgaccgtg tccaccacaa tcgtcatcat ctccctgaag atcttcgaca tcgtcttcac
     2341 gatgaccaac gggaactacg gaacggaagt gatcgcctcg gtgcagtaca agcagatgtt
     2401 ccgcttctac cactacgggc gcggctcggc gatcgcgatc gtcctcgtcc tcgccgtcac
     2461 cccggtgatg tggtacaacc tcagacagtt cgccaagcag gaggtgttca aatgaacaac
     2521 agaaaagcgt cgatcgcgat caacacggct cttctgatcc tggtcgtcat ctggttcatt
     2581 ccgatcctgg gcctcttcat ctcctcgttc cggcctcggg actacgtcct caccaccggg
     2641 tggtggaccg ccttcacgaa gaaccggatc ttcacccttg acaactaccg tcaggtgctc
     2701 ggcggcacca aatacacgtt cgtcgatgcg ctgggcaaca cggtgcgctc cagcggcgac
     2761 aacctctcgc aggcgttcat caactccttc accgtgacga tcccctccgt gatcattccg
     2821 atcctcatcg cggccgcggc ggcgtacggg ttcgcctgga tggtcttccc gggaaggaag
     2881 ttcttcttca cctcggtggt ggccctgctg gtcgttccgc tccagatcgc cctgatcccc
     2941 atcctgcgcg actaccagaa gatcgggctc accggatcgt acctcggcat ctggctcgcc
     3001 cacaccgggt tcggcctgcc gctctcgatc tatctgttat acaactacat ctccacgatt
     3061 ccacgcagca tcttcgaatc cgcctacctc gacggggcac ggcagatcgt cgtcttcgcc
     3121 cgcctcgtcc tgcccctctc cattcccgcg atcgcctcgc tcgctatctt ccagttcctc
     3181 tgggtatgga acgacctgct ggtcgccctg gtcttcctgg gcggaacgca ggatgtcgcg
     3241 gtcctcaccc agcgcctggc gacgatgatc gggtcccggg gacaggactg gcacctgttg
     3301 accgccgggg cgttcgtctc catggtcgtt ccgcttgcgg tgttcttcag cctgcagcgc
     3361 tacttcgtcg aaggcttgat ggcgggatcg gtaaaggggt aatacatgaa tacgaactgg
     3421 cttctcaccc gggaaggccc caacaggaac ggccacaccg aatcgctcta ctctctcggc
     3481 aacggccgcc tcggcttcag agggtttccg ctctggcggg atcccctctt ccaggggggt
     3541 gtcttcctca acgggctgta tgacacccgc ccgatccgct atgaagagga cgtctacggc
     3601 tcggcgaaga tcgcccagca catcgtcccc ctgcccgata gccggttcat cgccatccgg
     3661 atcaatgacg aaccgatcag accgatcaac gaacgaaaga ccctcgactt caagaccggc
     3721 atcctcaccc accaggtgga gacgaaggaa gcggtcatcg agtatgagac gttcctttcg
     3781 atgacccgta catcactggg tgtggtcacc ctgaacatcg agcagacagg gaacaacagg
     3841 atttcaatcc tctcaggcat cgcccaggat gaggtgtcaa caagcaacac cttcgacccc
     3901 cgggtggcga accatatccc gaagctccga accatcaatg cttctcacac cgacgacggc
     3961 ttcaccgccg agttcgaaac ggagaactcg aacctcgccg ttcatatgac cacaagtcac
     4021 cgctgtgaca tacaacccaa agaaaccacc tataacaacc ttccggcgct cctctatgag
     4081 acaacagaga agaacataga cctgaccaaa ttcttcagct acgaaacaga acggaaaccg
     4141 ctgtcccatc cttcctacga gaccctgaag gaagaacagg tcgcctacta tgagcggttc
     4201 tggcaaaaga gcgatgtccg gatacaggac aacccgaccc ttcagctggc gctgcggttc
     4261 aacatcttcc agctgaacca gtcgaccgga cacgcccttg cggccaaggg cctcaccggc
     4321 gtcgggtacg aggggcactc cttctgggac acggagatct acgcccttcc cttcttcacc
     4381 cacaccaatc cggaaaaagc ccgcgacctg ctgctctatc ggatcggcat gttgccgtat
     4441 gcgaaaaagc gggcaaggga gctttcccag aaaggggccc tgttcccgtg gcggacgatc
     4501 tacgg
//
LOCUS       DAOSLI010000100         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_17088_length_4494_cov_22.119847, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000100 DAOSLI010000000
VERSION     DAOSLI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17088_length_4494_cov_22.119847"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..2503)
                     /locus_tag="PLF67_09100"
     CDS             complement(167..2503)
                     /locus_tag="PLF67_09100"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOE90206.1"
                     /translation="MITNKDRLFNLSTKRTTYLFAVTETGHLEHLYWGRRLTKDVSID
                     AFAEKRSIGIIGETAYDSDHPTLFLSNLAMEYSTTGKGDYRESSIDIEYQKGLRTLDF
                     TLQSFRILKGKPRLFGGLVESYGDHEQCTTLEITLREKLLPLRILLTYTTFEDTDVIT
                     RRTTLHNDGHESVVIRNLASAQVDLEGSWELITFDGAWGRERYVNRRELKPGIYINDS
                     KKGVSSSDHNPAIFLTNEENECVGFNLVYSGNHRELVEVSPFGKTRVMTGINPTGFSW
                     TLTSGERFQTPEAVMTYSPHGLEGASHNFHQFINHHIIRGPWKFRERPILINNWEATY
                     FNFTEDKILKLARESAELGIELFVLDDGWFGVRNDDTTSLGDWTVNSKKLPSGLGYIS
                     SQIKKMGMLFGLWVEPEMVSIQSELYKNHPDWMIAIPGRTPGVGRNQYILDLTREDVR
                     RYLFETLSKTWKLADVNYIKWDMNRVFSDLYSLNPEMANHGEFLHRYVLGLYELLTKL
                     ATEFPAVLFESCASGGNRFDLGMLCFMPQTWTSDNSDALSRLYIQEGTSYAYPLSTMG
                     AHVSSSPNHQTLRRTDLESRFNVAAFGVLGYELDVTSLNRQQKEAIKAQIAFYKAHRS
                     ILQYGTFTRIKLANSASNQVVWAVASKDRSELLVLFAQKLNRANPGGDILRVHAVDLN
                     ATYEVFPRQQKIDIKVFGDLLNMVSPVHITEGGIAQETISKALSLDSEVEHYRLSGEQ
                     IAFGGIKLNQQFGGTGYDSMTRVLGDFGSRIYLFKRID"
     gene            2551..3672
                     /locus_tag="PLF67_09105"
     CDS             2551..3672
                     /locus_tag="PLF67_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90207.1"
                     /translation="MDIRRLKLFNSRNAALLTNDLVQLVVEDQGGMVLEFSAKIANGG
                     RINPHAIPSHRGTGSSVYSDENGEFWQNNPTLYQQAGSYLVFPREDEHSTAASYWLVE
                     QYGTDPEFGGVWLTSRMRNRLEGWQIRKLDLLLPNQPVHYTAYQVTNKAPEPLQATAQ
                     HRTLLGPPFLEQGCVVNTNAASFESFPIPNTQSRIPAGQFNDWKKIQTRENGLLDLTE
                     IPKVTNTVDFIKAALNQDAPLGFTSMINPRMQMAYVTFFPSKPEDEDELVFTHTSFLY
                     QLGGMSQTPYALYAGGLDQQYTVELGEEFSTTLKPLQTRIFFHARAFAPYENARLGGN
                     FHSLEQTLEGILLKRTKTTALIPADPSFHAARTLAKRLF"
     gene            complement(3738..4250)
                     /locus_tag="PLF67_09110"
     CDS             complement(3738..4250)
                     /locus_tag="PLF67_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90208.1"
                     /translation="MRKGMILTAIIILLGPLALFALPSTVTFNIASTVALINQIQIKG
                     ANEDNSAYGSSFTFNFDELPQVDNRYIADLKYTTNSRTGVTLGIIGSELKSTENKVIP
                     YTLTLGNSTTIKVGYDEDNNPEGTIPTPSQFTQLTTTTAPVYLTIPGSVSDYPAGAYT
                     GTITFTLGAK"
BASE COUNT          905 a         1168 c         1378 g         1043 t
ORIGIN      
        1 ccgtcctgcc cgtcgaagtc gatcgcttcg ggaacgccct ggccttcgac gaacacgact
       61 cttctgatcg cctggcagtg aaggaggtag gaagggtggt cgtcaccctt ttggaggtag
      121 tagatcgtga cgctcatgag tgggagtgta caaaggtggg gcgggttcag tcaattcgct
      181 tgaagaggta gatccgggaa ccgaagtccc ccagaaccct tgtcatcgaa tcatagccgg
      241 tgccgccgaa ctgctggttc agcttgatcc ctccgaaagc gatctgctcg ccgctgaggc
      301 ggtagtgctc gacctcgctg tcaagggaga gcgccttgct gatcgtctcc tgggcgatgc
      361 cgccttcggt gatgtggacc ggggagacca tgttgagcag gtcgccgaac accttgatgt
      421 cgatcttctg ctgtctgggg aacacctcgt acgtcgcgtt gagatcgacc gcatggactc
      481 gaaggatgtc gcctccgggg ttggcccggt tcagcttctg ggcgaacagg accaggagtt
      541 cgctcctgtc cttggacgcg accgcccaga ccacctggtt ggaggcgctg ttcgcaagct
      601 tgatgcgggt gaaggttccg tactgcagga tcgagcggtg ggccttgtag aaggcgatct
      661 gggccttgat ggcctccttc tgctggcggt tcagcgaggt gacgtccagt tcgtatccga
      721 gcactccgaa cgccgctacg ttgaagcggc tttccagatc ggtccttcgg agcgtctggt
      781 ggttcggaga actggaaacg tgcgctccca tcgtcgagag gggataggcg tagctggtcc
      841 cctcctggat gtagaggcgg gagagggcgt cgctgttgtc gctggtccac gtctggggca
      901 tgaagcagag catccccagg tcgaagcggt ttccgccgct ggcgcacgat tcgaagagga
      961 ccgccgggaa ctcggtggcg agcttcgtga gcagctcata gaggccgagg acgtagcggt
     1021 ggaggaactc cccgtggttg gccatctcag gattgaggct gtacaggtcg ctgaagacgc
     1081 ggttcatgtc ccatttgatg tagttcacgt cggcgagctt ccacgtcttt gaaagggtct
     1141 cgaagagata cctgcggaca tcctcccggg tgaggtcgag gatgtactgg ttccggccga
     1201 cgccgggcgt ccttcccggg atcgcgatca tccagtcggg gtggttcttg tagagttcgc
     1261 tctggatcga gaccatctcc ggctcgaccc agaggccgaa gagcatcccc atctttttga
     1321 tttgtgatga aatatacccg aggcccgagg gcagcttctt ggagttcacg gtccagtcgc
     1381 ccaggctcgt ggtgtcgtcg ttgcggaccc cgaaccagcc gtcatcgagg acgaagagtt
     1441 caatgcccag ctcggcgctt tcccgggcga gcttcagaat cttgtcttcg gtgaagttga
     1501 agtaggtggc ttcccagttg ttgatgagga tcggccgctc gcggaacttc cacggtccgc
     1561 ggatgatgtg gtgattgatg aattggtgga agttgtgcga agccccctcg agtccgtggg
     1621 gggaataggt catgaccgct tcgggggtct ggaagcgctc gccgcttgtg agcgtccagc
     1681 tgaagcccgt ggggttgatg ccggtcatga cccgggtctt cccgaacggg gagacttcaa
     1741 caagctcgcg gtggttgccg ctgtagacga ggttgaaccc gacgcattca ttttcttcgt
     1801 tggtcaggaa gatcgcgggg ttgtggtcgg aagagctgac gcctttcttc gagtcgttga
     1861 tgtagatccc gggtttcagt tcccgcctgt tcacgtatcg ctcccgcccc caggcgccgt
     1921 cgaaggtgat aagctcccaa gagccttcca agtcaacctg ggccgaggcg aggttgcgga
     1981 tgacgacgct ctcgtggccg tcgttgtgga gcgtcgtgcg acgggtgatc acgtcggtgt
     2041 cttcaaaggt ggtgtaggtc agcaggattc gaagcggaag cagcttctcc ctgagggtga
     2101 tctccagcgt ggtgcattgt tcatgatcac cgtaggattc gaccaggccg ccgaagagcc
     2161 ggggcttgcc tttcaggatt ctgaagctct gaagggtgaa gtcgagggtt ctcagccctt
     2221 tttggtattc gatgtcgatc gaactctctc gatagtctcc tttgcctgtg gtagaatact
     2281 ccatggcgag attggagaga aacagcgtgg gatggtcact gtcataggcg gtttcaccga
     2341 tgatgccgat gcttctcttt tcggcgaatg catcgatgct tacgtctttg gtgagccgac
     2401 gaccccaata gaggtgctca aggtgtccgg tttcggttac tgcgaagagg taggtggtcc
     2461 gcttggttga aaggttgaag agtcggtctt tgttggtaat catctgtcgc tccttgttct
     2521 agagtatacc gtctttttgg agagttagga atggatatac gtcgtttgaa actgttcaac
     2581 agccgcaatg ccgcgctctt gacgaatgat ctggtccagc tggtcgtcga ggaccagggc
     2641 gggatggtgc tggagttcag cgcgaagatc gcaaacggcg ggcggatcaa cccgcatgcg
     2701 atcccttccc accggggaac gggttcttcg gtgtactccg acgagaacgg cgagttctgg
     2761 cagaacaatc cgaccctcta tcagcaggcg ggttcatatc tggtgttccc cagggaggac
     2821 gagcattcga ccgccgcatc ctattggctg gtcgagcagt acggcaccga tcccgagttc
     2881 gggggagtgt ggctgaccag ccggatgcgc aaccgcttgg aaggatggca gatccggaag
     2941 ctggatctgc ttctgcccaa tcagccggtc cactacaccg cctatcaggt caccaacaag
     3001 gctcccgagc cgctacaggc gacggcgcag catcggacgc tgcttggccc tccctttctg
     3061 gaacaggggt gcgtggtgaa caccaacgca gcgagcttcg aatcgtttcc gattcccaac
     3121 acccagagcc gaatccccgc ggggcagttc aatgactgga agaagatcca gaccagggag
     3181 aacggccttc tggatctcac cgagattccc aaggtcacca acaccgtcga tttcatcaag
     3241 gcggcattga atcaggatgc ccctctgggc ttcacctcga tgatcaaccc gaggatgcag
     3301 atggcgtacg tcacgttctt tccctccaag ccggaggatg aggatgagct tgtcttcacc
     3361 catacctcgt ttctctatca gctgggcgga atgagccaga ctccctatgc gctctatgcc
     3421 ggagggctcg accagcagta cacggtcgag ctcggtgagg agttctcgac gaccctgaag
     3481 cctcttcaga cgaggatctt cttccatgcc cgggcgttcg ccccgtatga gaacgcccgc
     3541 ctgggtggaa acttccattc cctggaacag accctggagg gcatcctgct caaacggacg
     3601 aagaccactg ccctcattcc ggccgatccg tcgttccatg cggcaaggac tctggcaaag
     3661 agattgttct aaggctaaag gcgaaagaaa aagcccttcc gattgagggc tttgaaaagt
     3721 gcgtgaaaaa gattcggcta tttcgccccg agggtgaagg tgatcgttcc cgtataggcg
     3781 ccggccgggt agtcgctgac gctgcccgga atggtgagat agaccggtgc ggtcgtcgta
     3841 gtcagctgag tgaactggga gggggtcgga atcgtacctt ccggattgtt gtcctcatca
     3901 taccccacct ttatcgttgt gctattcccc aaggtaagag tgtaggggat gaccttgttt
     3961 tcggtgcttt tgagctcact tccgatgatt cccagagtca ccccggttct ggagttggtg
     4021 gtatatttga ggtcggcgat ataacggttg tccacctggg gcaactcatc gaagttgaac
     4081 gtgaaggatg atccgtatgc cgaattatct tcattcgccc ccttgatctg gatctggttg
     4141 atcaaggcga cagttgaggc gatattgaac gtgacggtcg aaggaagcgc aaacagggca
     4201 aggggcccta gaagaatgat gatggcggtg agaatcatgc cctttctcat atccggtctc
     4261 tccatgtggg gatcttctcc ccggtgcaag tagtcggatt ctttccctga atccgcttta
     4321 agcataataa agattatcac ctatgtcaac tggaagatga tgataatatc atctatttga
     4381 agccgaaaga gacatgttgt taattaattg ttatattgta agcaataatt atttttatta
     4441 aaacaaaaac atatgacaag atttagacat gaaaaggtct tgagtacaaa aaaa
//
LOCUS       DAOSLI010000101         4453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_17277_length_4453_cov_26.391314, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000101 DAOSLI010000000
VERSION     DAOSLI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17277_length_4453_cov_26.391314"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLF67_09115"
     CDS             complement(<1..887)
                     /locus_tag="PLF67_09115"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOE90209.1"
                     /translation="MSLIDRAYGSLLGLMVGDAFGAQVEGTSGAMLKELFPFGIREMG
                     SRIRSFEGGTVTDDSEMALLMAASLVANDGFNVVDLRRRYVAWIDTDPPGIGSTTYQA
                     LKNDYFLHLRWTNGALMRVAPLGIWGTRVSEEELLEIAREECKLTHVHRFCQEANQLW
                     ALGLRKAILEGSSKEEIYTYLLELADRIEIDPSLKKVLVLAGTQPPVVDGPDKGWVTV
                     SFQQALYSVLHATSIEDEIRAITMRGGDTDTNAAIYGMMAGAIHGSEAIPRRWVEALK
                     PSSSLLELLGSRAAKLDALA"
     gene            complement(896..1708)
                     /gene="ccsB"
                     /locus_tag="PLF67_09120"
     CDS             complement(896..1708)
                     /gene="ccsB"
                     /locus_tag="PLF67_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOE90210.1"
                     /translation="MYQTIENTAFMIAFIFYVASALLFFVRISKLAKRVEKGAILTTS
                     LGFLSHTAAMIARTIGAGRLPFSNQYEFALSFAWGIVLSFLILYAIYRFGAIGAFVLP
                     LAILVMGYASLQSKTVRPLMPALQSMWLTFHVGTAILSYGSFALGCAISVIYLIDSKR
                     SEEHKILSDPEVSDILAYRVVVFGFLMLTLVIISGAIWAQKAWSRYWAWDPKETWSLI
                     TWIIYAIYLHLRLRRNWKGKHAAWFSIIGFISVIFTFIGVNILLPSIHSYRA"
     gene            complement(1692..2957)
                     /locus_tag="PLF67_09125"
     CDS             complement(1692..2957)
                     /locus_tag="PLF67_09125"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOE90211.1"
                     /translation="MKNRTSKFIRFLASLKVAIVLLLLIGGYIVIGTVLPQHSAQTWY
                     LERYPAMGKLILGLSLDTAYSSPVFIILLVLFSINLALCTILSLKGQVRQVKPTFFPR
                     FKEEEYGFEGVDADKVRSYLKKHRYRVTEEDGVIRAAKHRYGVLGAAITHLGIIILFL
                     GGAIGTMSSSEEMITLLPGNRHRFEKQGFTLTLDDFHMTFEPNGAVKQYYSDVTVVDD
                     DGTTLSETMWVNKPFHHNGLGFYQANYGWTSHLQISDSESGEVVAEGLIRSGKTYFHQ
                     PNHLTIYLYGYYPELGIGHDQQPVKLSDREIDPYYAVVLYEFGQPVGSYILAPNQHIS
                     YENLSITFTHSIAYTGLLVRSDLSYPIVLVSFITIILGLFVSFYLYPRFVTYKDGRII
                     TSSRRNEWIFHRTITTALAKKDNTYVSND"
     gene            complement(3027..4082)
                     /locus_tag="PLF67_09130"
     CDS             complement(3027..4082)
                     /locus_tag="PLF67_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90212.1"
                     /translation="MSTAHGKLGCVTCHGGNGDEGRFIHAHDGVDPRPSADVNGICAQ
                     CHQEVTDTFATSLHATVRGFSNSLIEFSGDPNALDDLHKGLGEVYKLNCMNCHASCGE
                     CHVSRPDSYAGGLIDQHKFFSTPPMDQTCFACHGMRNAGEFMGTVGFARDVHYEMGMT
                     CVDCHAVSNFHGTGTAYDSMWDKPTLPSCSDCHGDVLSGNSEIKMHNVHGDALACQVC
                     HGQANQNCFECHVTIADDRQSLASHSETRILFRIGLNTDPTPERPYKYVALRHMPTTA
                     DSFIEAGDNLLPNFDEKANWKYSPTHNIQRSTFQNESCNACHGNPRIFLSEKDLRETD
                     SKANWEIVPPVPAALGR"
     gene            complement(4140..>4453)
                     /locus_tag="PLF67_09135"
     CDS             complement(4140..>4453)
                     /locus_tag="PLF67_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90213.1"
                     /translation="LWLLKVYRYCYNGLSTLGGDMGKHRLKLSRMGLLALGVLLLFVG
                     TGCSKKKEAVAETVEVTARFTKPVAIASQSSTKCIACHTQVGPINNLATPVSAAAETG
                     G"
BASE COUNT          904 a         1250 c         1348 g          951 t
ORIGIN      
        1 gccaatgcat ccagctttgc tgctctgctt ccgaggagct cgagcaggga ggagcttggt
       61 ttcaaggctt cgacccatcg tcggggaatc gcttcggagc catgtatcgc accggccatc
      121 atcccgtaga ttgccgcatt ggtgtcggtg tccccgcccc gcatcgtgat cgccctgatt
      181 tcatcttcga tgctcgttgc atgcagaacg ctgtacagcg cttgctggaa tgagacggtt
      241 acccagcctt tgtccggtcc gtcgacgacc ggcggctgcg ttccggcaag cacaagcacc
      301 tttttcaggg aaggatcgat ctcgatgcga tcggcaagtt caagcagata ggtatagatc
      361 tcttcctttg atgatccctc caggatcgct ttccgaagcc ccagcgccca cagctggttc
      421 gcctcctggc agaatcggtg gacatgggtc agcttgcact cttcacgagc gatctccaga
      481 agctcttcct cgctgactcg ggttccccag atacccagag gagccaccct catcaacgcc
      541 ccgttggtcc atctgagatg caggaaatag tcgttcttca gcgcctgata ggtggtgctg
      601 cctatgcccg gcgggtcggt atcgatccag gcgacatagc ggcgccgaag atcgacgaca
      661 ttgaacccgt cgttggcgac caggctggcc gccatcagca gggccatctc gctgtcgtcg
      721 gtgacggtac cgccttcaaa ggatcggatc ctgctaccca tctcccggat tccgaagggg
      781 aagagctctt tgagcattgc gccggaagtg ccttcgacct gagcgccgaa cgcatcgccc
      841 accatcaagc ccagcaatga accgtacgcc cggtcgatga ggctcatcat gcaccttatg
      901 ctctgtagct gtggatgctc ggcagaagga tgtttacgcc gatgaaggtg aagatgacgg
      961 agatgaagcc gatgatcgag aaccaggccg catgcttgcc cttccagttt cttctcagcc
     1021 gaaggtggag gtagatcgca tagatgatcc aggtgatcag ggaccaggtc tccttgggat
     1081 cccacgccca gtagcgggac caagcctttt gcgcccagat cgctccgctg atgatgacga
     1141 gcgtgagcat caggaagccg aagaccacca cccggtaggc gaggatgtcg ctgacttcgg
     1201 gatcggatag gatcttatgc tcttcgctcc gcttcgagtc gatcaggtag atcacgctga
     1261 tcgcgcaccc gagggcgaag gagccgtagg agaggatcgc tgtccccacg tggaaggtga
     1321 gccacatcga ctggagggcg ggcatgagcg gccggacggt cttgctctgc aaagaagcat
     1381 accccatcac cagaatcgcc agaggcagca cgaacgcccc gatcgccccg aagcggtaga
     1441 tcgcatagag gatcaggaag ctcagcacga tcccccaggc gaaggagagg gcgaactcgt
     1501 actggttgct gaacggcagc cgacccgccc caatcgtccg ggcgatcatc gcggccgtat
     1561 gggaaagaaa cccaagggag gtcgtgagga tggcgccttt ttccacccgc ttcgccagct
     1621 tggagatccg gacgaagaag aggagggcgg aagcgacata gaagatgaag gcgatcatga
     1681 atgccgtgtt ttcaatcgtt tgatacatat gtgttatcct ttttcgccaa ggcggttgta
     1741 atcgttcgat ggaagatcca ttcgtttctt cggctggacg tgatgattct accatctttg
     1801 taggtgacaa acctcggata gaggtagaag ctgacaaaga gaccgagaat gatcgtgatg
     1861 aaggagacga ggacgatcgg gtaggagagg tcgcttcgaa ccagcagtcc cgtataggcg
     1921 atgctgtggg tgaaggtgat cgacaggttc tcatagctga tgtgctggtt cggggcgagg
     1981 atatacgatc cgaccggctg gccgaactcg tagagcacga ccgcatagta cgggtcgatc
     2041 tcccggtcgc tcagcttcac cggctgctgg tcatgtccga ttccgagctc ggggtaatat
     2101 ccgtagagat agatcgtcag atggttcggc tgatggaagt acgtttttcc gcttcgaatc
     2161 agcccctcgg ccacgacttc gccgctttcc gaatcactga tctgcagatg gctcgtccac
     2221 ccgtagttgg cttggtagaa cccgaggccg ttgtggtgga agggtttgtt cacccacatg
     2281 gtctcgctca gagtggttcc gtcgtcgtcg acgaccgtca cgtcgctgta gtactgtttc
     2341 accgccccgt tgggttcaaa ggtcatatgg aagtcgtcga gggtgagggt gaagccctgc
     2401 ttctcgaagc gatgccggtt tcccggcagc agtgtgatca tctcctcact ggagctcatc
     2461 gtcccgatcg ctccgccgag gaagaggatg atgattccca ggtgggtgat cgccgccccg
     2521 agcacgccgt agcggtgctt ggccgcccgg atcaccccgt cctcttccgt cacccggtag
     2581 cggtgcttct tcaggtatga gcggaccttg tcggcatcga caccctcgaa tccgtattct
     2641 tcttccttga aacggggaaa gaaggtcggc ttgacctgac gcacctgccc cttcaggctg
     2701 aggatcgtgc agagggcgag gttgatggag aagaggacaa ggaggatgat gaagaccggg
     2761 gagctgtacg cagtatcaag ggacaacccg aggatcagtt tccccatcgc cgggtagcgc
     2821 tcaaggtacc aggtctgggc gctgtgctgc ggcagcacgg tgccgatcac gatatatcct
     2881 ccgatcagga gcaggaggac gatcgccacc ttcagggagg cgaggaagcg gatgaatttc
     2941 gatgttcgat tcttcatact gtctttatac aacacaagag ccaaaagctt ccacctttgg
     3001 ctcttgaaaa accttcgatt accgacttac ctgccgaggg ccgccggcac cgggggaacg
     3061 atttcccagt tggccttgct gtccgtttcc ctcagatcct tctcgcttaa gaagattcgc
     3121 gggttcccgt gacatgcatt gcagctttcg ttctggaagg tcgagcgctg gatgttgtgg
     3181 gtcggcgaat acttccagtt cgccttctcg tcgaagttgg gcagcaggtt gtcgcccgct
     3241 tcgatgaagg agtcggcggt ggtgggcatg tgccgcagcg ccacgtactt gtacggccgt
     3301 tccggggtcg ggtcggtgtt cagcccgatc ctgaagagga tccgggtctc cgagtggctg
     3361 gcaagggact gccggtcatc cgcgatggtg acgtggcact cgaagcagtt ctggttcgct
     3421 tggccgtggc agacctggca cgccagcgca tcgccgtgga cgttgtgcat cttgatctcg
     3481 gaattgccgg aaagcacatc accgtgacaa tcactgcagg agggcagggt gggcttatcc
     3541 cacatcgagt cgtacgccgt tccggttccg tggaagttgc tgacagcgtg acaatcgacg
     3601 cacgtcatgc ccatctcgta gtggacgtcc cgggcgaagc cgaccgttcc catgaactcg
     3661 ccggcgttcc gcatgccgtg acaggcgaag caggtctggt ccatcggcgg ggtgctgaag
     3721 aacttgtgct ggtcgatcag gccgccggca tagctgtccg gccgcgatac gtgacactcg
     3781 ccgcaggagg cgtgacagtt catgcagttg agcttgtaga cttcacccag acccttatgg
     3841 agatcgtcaa gggcgttggg atcgccggag aactcgatca gggagttgct gaacccgcga
     3901 accgtggcgt gcagggatgt cgcaaaggtg tccgtcactt cctggtggca ctgggcgcag
     3961 atgccgttga catcggccga cggtcggggg tcgaccccgt cgtgggcatg gatgaagcgg
     4021 ccttcatcgc cattgccgcc gtggcaggtt acacagccca gctttccgtg ggcggttgac
     4081 aaaaattcag ttgaaacaag gaatttttca agcgccgaaa actccggcgc ggcaggagct
     4141 caccctcctg tctctgccgc ggcagagacg ggtgtcgcaa gattgttgat cggcccgacc
     4201 tgagtatggc aggcgatgca cttggtgctc gattgagagg cgatcgcgac gggtttcgta
     4261 aagcgcgcgg ttacctcgac ggtctccgct acggcttcct tcttcttgct acagcctgtc
     4321 cccacaaaga gcaacagcac gccaagtgcc agcaatccca tgcgggagag tttcagtcga
     4381 tgttttccca tatctcctcc caatgttgac aacccattat aacagtatcg ataaactttt
     4441 aacaaccata aaa
//
LOCUS       DAOSLI010000102         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_18937_length_4152_cov_26.646815, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000102 DAOSLI010000000
VERSION     DAOSLI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18937_length_4152_cov_26.646815"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1140
                     /locus_tag="PLF67_09140"
     CDS             229..1140
                     /locus_tag="PLF67_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE90214.1"
                     /translation="MAGTATSLHKQMTKRARRDMWTFFAFTGPTFILLAIFIFWPIFY
                     SFYLSLFKWNMVSPRKTFLGLQNYKDMFSDDVFKLVVKNTLIMAFFTVVVKMAISLYL
                     ATQLNKKIRLSNFYRAIIFSPTFTANVAIAMVWAWIFEPTYGLMRSFLRPLGLPMIDW
                     LKSTSHSLPAVIIVLIWSGIGYDMVLFLAALKNVPLEIYEAAIVDGANPFQTFWKITF
                     PLISPTTFFLTITGFINVLKAFDIARIMTEGGPLNSSNVYVHYLYQNAFQWFRAGYAS
                     ALALVLFLFIMLITLIQNRLSKSWVHY"
     gene            1154..1996
                     /locus_tag="PLF67_09145"
     CDS             1154..1996
                     /locus_tag="PLF67_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE90215.1"
                     /translation="MEQHVNIRALASKLFTHFILIIATMVIGLPFLWMLSTSIKGPTE
                     VAMFPPVWWPKEVRLDNFTTAWNTAPFGRFYINSIVTAAAGVILGVVTAAFAAYAFAR
                     IRFKYRDFFFLVMLAAMMIPGQVALIPNYVILAKLNWINTYQGIVVPHIANVFGAFLL
                     RQYFMTIPNDLYDAAQIDGLGHTRTLLHVALPLSRPVLGTLLLYLFIQKWNAYLWPLI
                     VTNKQNMRTLPVGLAMIRSAEYSIGPEHLMAASIFVLMPVLIAYFLAQKQLIEGIAAG
                     AVKG"
     gene            2050..3324
                     /locus_tag="PLF67_09150"
     CDS             2050..3324
                     /locus_tag="PLF67_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE90216.1"
                     /translation="MKKGILLLVVMLIATTFLFAAAKAEEQKPDRIEILYWRGLTGVA
                     GEAQEEIARRFNESRDDIHVTVEFQGAYAEILQKMQAALAAGDVPDLVLLDSPHLQIF
                     AKDEAVQNLDRFVKAEPKGFLDDYVPGLLADSYYNGSLYALPALRSTPLLYVNADAFV
                     EAGLPKRAPKTWEEFREFAKKLTKVNERGETTQYGVGFTLGATTAHWYFQGAVYAYDG
                     KVSDEKFNMYLHEEPAVKAATLWQEMVFKDKTGVPSTTHDDLFTGKVAMVFGSTGSMG
                     NVMRRASFEVIPAFIPGQVRNGVPVGGSVIAMPSTDKWRQWAAWEFLKFFTSPESQAY
                     LAEMTGYMPSRISSAKVPSLVEYYNKNPHWQVAIDQLKYVQPQASVISLPKGTEILRQ
                     MVEKLLIGNQDVRKVMAETEADLTKEYNENFK"
     gene            3308..>4152
                     /locus_tag="PLF67_09155"
     CDS             3308..>4152
                     /locus_tag="PLF67_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOE90217.1"
                     /translation="MRTSSKHWISIITMAASAAIVLLILNSCTTVAIKEVDMGKTKAV
                     AHRGGALLAPENTLEAFQAGIASGADQLELDIHLSKDDVIIVMHDPTLQRTANQSGAI
                     RDYTAAELKQFNAASAHPGHFPLEIPTLKEVLDLVKDTDLELQIEIKVDKDGNRYEGI
                     EERLIALLKEYELIDRSIVISFDFPTLARIRELQPSLRTGALVSKAYMSKIGSKGPKA
                     VAEGIKALQVDYIGINHTYLSPALHQELRNQELGIGAWTVNDEEAMKKIAALGVAFIT
                     SDRPDL"
BASE COUNT          942 a         1196 c         1094 g          920 t
ORIGIN      
        1 cccttcaaca ggaaaatcat atgcccataa aggcgggtgg aagacttgcg tgattctgaa
       61 agagcggtta tcctattgaa gtagcatcga tgatgtcggc ttcgctgatg gcggaacacc
      121 tgagtttgga ggattggata tagctcagcg acatattccc cggaacatta ctttaaccaa
      181 ccgttggatg agtccattga gtaacagctc acccaaggag gaacaccagt ggcaggtaca
      241 gctacatctt tacataagca gatgaccaaa cgggcaaggc gcgatatgtg gacattcttc
      301 gcgttcaccg gccccacgtt catcctgttg gccattttca tcttttggcc aatcttctac
      361 tcattttact taagcttgtt taagtggaac atggtcagcc cgagaaaaac attccttggc
      421 ctgcagaact acaaggatat gttcagtgat gacgtgttta aactcgtcgt gaagaacacg
      481 ctcatcatgg cgttcttcac cgtcgtggtg aagatggcga tcagcttgta cctggcaacc
      541 cagctgaaca agaagatccg cttgagcaac ttctaccggg cgatcatctt cagccccacc
      601 ttcaccgcga acgtggcgat cgcgatggtc tgggcgtgga tctttgaacc cacctacggg
      661 ctgatgcgtt ccttcctcag accgttgggc ctgccgatga tcgactggct caaatcgacg
      721 agccactcgc ttcccgcggt gatcatcgtc ctgatttgga gcggaatcgg ctatgacatg
      781 gttctcttcc ttgccgcgct gaagaacgtt ccgcttgaga tctacgaggc ggccatcgtc
      841 gatggtgcga accccttcca gaccttctgg aaaatcacct ttccgctgat cagtccaacg
      901 accttctttc tgacgatcac cggattcatc aacgtgctca aggcgttcga catcgccaga
      961 atcatgaccg agggaggacc gttgaactct tcgaacgtct acgtccacta cctctatcag
     1021 aatgcattcc agtggttcag agcggggtat gcctccgccc tcgccctggt gcttttcctc
     1081 ttcattatgc ttatcacctt gattcagaat cggctctcca agagctgggt tcactattga
     1141 tcggaggagc aggatggaac aacacgtcaa tattcgagcc cttgcttcga agctcttcac
     1201 ccatttcata ctcatcatcg cgacaatggt catcggcctg cccttcctgt ggatgctctc
     1261 cacctcgatc aagggcccga cggaagtcgc gatgttcccg ccggtctggt ggccgaaaga
     1321 ggttcggctg gataacttca caaccgcctg gaacaccgca ccgttcggcc ggttctacat
     1381 caacagcatc gtaaccgcgg cagcaggggt cattctgggc gttgtcaccg cggcattcgc
     1441 cgcgtatgcg tttgcccgca tccgcttcaa gtatcgggac ttcttctttc tcgtgatgct
     1501 ggccgcgatg atgattcccg gccaagtcgc cttgattccg aactacgtca tcctcgccaa
     1561 gctgaattgg atcaacacct atcagggaat cgtggttccg catatcgcaa acgtgttcgg
     1621 agcgttcctc ctgcgccagt acttcatgac gatacccaac gacttgtacg atgcggccca
     1681 gatcgacgga ctggggcaca cgcgaacgct tcttcacgtc gcccttcccc tctcccgtcc
     1741 ggtcttggga acgcttctcc tctacctgtt catccagaag tggaacgcct acctctggcc
     1801 gctgatcgtt acgaacaaac aaaacatgcg aaccctgccc gtgggattgg cgatgattcg
     1861 aagtgctgag tactcgatcg gaccggaaca cttgatggct gcttcgattt tcgtgttgat
     1921 gccggtattg atcgcgtatt tcctggctca gaaacaattg attgagggta tagcagctgg
     1981 cgccgtcaag ggctagctca tacaaatcgg caacccctac cggatgggga catttccaag
     2041 gaggaaataa tgaaaaaagg tattttactt cttgttgtaa tgcttattgc aacaaccttt
     2101 ctcttcgcag ccgcaaaggc cgaggagcag aaaccggatc gaatcgagat tctctactgg
     2161 cgcggtctca ccggagtagc cggagaagct caggaagaga tcgcccggcg ctttaacgaa
     2221 tcccgtgatg atattcacgt gaccgttgag ttccaaggcg cgtatgctga gatcttgcag
     2281 aagatgcagg cggctctcgc tgccggcgat gtgccggatc tcgtcctgct cgattcaccg
     2341 caccttcaga tttttgccaa ggatgaggcg gtccaaaacc tcgatcgctt cgtcaaggcc
     2401 gaacccaagg gattccttga tgactatgtc ccgggtctct tggcggacag ctactacaac
     2461 ggaagcctct atgcccttcc ggctcttcgc tcgactccct tgctgtacgt caacgccgac
     2521 gccttcgtcg aggcgggtct tccgaagcgg gctccgaaaa cctgggagga gttccgcgaa
     2581 ttcgccaaaa agctgacgaa ggtcaacgaa cgcggtgaaa ccacccagta cggcgtcgga
     2641 ttcaccctcg gagcgaccac cgcgcactgg tatttccagg gtgccgtata cgcatacgac
     2701 ggaaaggtca gtgacgagaa gttcaacatg tacctccatg aggagccggc cgtgaaagcc
     2761 gcgacgctct ggcaggagat ggtcttcaag gacaagaccg gcgttccttc caccacgcac
     2821 gacgacctct ttaccgggaa agtcgccatg gttttcggtt ccaccggttc gatgggcaac
     2881 gtcatgaggc gggcaagctt tgaagtgatt cccgcattca ttccgggtca agtccgcaat
     2941 ggagtgccgg ttggcggttc cgtcatcgca atgccttcca ccgacaagtg gcggcagtgg
     3001 gcggcatggg agttcctgaa gttcttcacg agccccgaaa gccaggccta tctcgccgag
     3061 atgaccggct acatgccctc gcgcatttct tcggcgaagg ttcccagcct ggtcgagtac
     3121 tacaacaaga acccgcactg gcaggtggcg atcgatcagc tgaaatacgt ccagcctcag
     3181 gcgagcgtca tctccctgcc caagggcacc gagatcctcc gccagatggt cgaaaagctt
     3241 ctgatcggca atcaggatgt ccgcaaggtc atggccgaga ccgaagccga cctgaccaag
     3301 gaatacaatg agaacttcaa gtaagcattg gatttcgatc atcacgatgg ccgcatccgc
     3361 ggccatcgtc ctcttgatcc tcaacagctg caccacggtc gccatcaagg aggtcgatat
     3421 ggggaaaacc aaggcggttg cccatcgcgg cggagctctt ctggctccgg agaacacgct
     3481 cgaagcgttt caggcgggaa tcgcttcggg ggcggatcag ctcgaactgg acatccatct
     3541 ctcaaaggat gacgtgatca tcgtgatgca cgatccgacc ctccagcgaa ccgcaaacca
     3601 aagcggagcg atccgcgact acaccgcagc ggaattgaag caattcaacg cggcaagcgc
     3661 tcatccgggg cattttccgc ttgagattcc gaccctgaag gaggtcttgg atctcgtgaa
     3721 ggataccgac cttgagcttc agatcgagat caaggtggac aaggacggaa acaggtatga
     3781 aggaatcgaa gagcgcctca tcgcgcttct taaggagtat gagctcatcg accgctcgat
     3841 cgtcatctct ttcgattttc cgacgctcgc ccggatccgg gagctccaac cgagcctcag
     3901 aaccggtgct ctcgtcagca aagcgtatat gagcaagatc ggttcaaaag gccccaaagc
     3961 cgtcgctgaa gggatcaaag cgcttcaggt ggactacatc ggcatcaacc atacctatct
     4021 ttcccccgct ctgcatcagg agctgaggaa tcaagaactg ggtatcggtg cctggacggt
     4081 gaacgatgag gaagcgatga agaagatcgc cgccctcgga gtcgccttca tcaccagcga
     4141 ccggcccgac ct
//
LOCUS       DAOSLI010000103         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_19474_length_4057_cov_10.786857, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000103 DAOSLI010000000
VERSION     DAOSLI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19474_length_4057_cov_10.786857"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PLF67_09160"
     CDS             complement(<1..129)
                     /locus_tag="PLF67_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOE90218.1"
                     /translation="MKIYAHRGYSGAYPENTMLAFAKALEANADGIELDVQLSKDGH"
     gene            423..1331
                     /locus_tag="PLF67_09165"
     CDS             423..1331
                     /locus_tag="PLF67_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE90219.1"
                     /translation="MRAMQRKGDAPKVWKGFPGAVEPYLFLVPSFIIFGLFLFYPFFR
                     TIYLSAFLTNKTGQPKIFVLFTDMDNYLALFRDPSFWKSVGVTFQFVFLVAFGGLLVG
                     LTTSLLTEKKFPGSDAATAVYAMPIAIASAAASMAFKMILHPTNGLINNVLGTQINFT
                     GDVRYALASVVGVTIWLTSGINFIFISAGLRNVPAELYDSASVDGAGYWRKVWNVTIP
                     TISPTLFFQIIINIIGAFQSFSQIKLLTEGGPSNTTNVMVWSIYRDAFFNFRFGSAAA
                     RSVILFLIVLGITIIQFRFEKKGVNY"
     gene            1331..2170
                     /locus_tag="PLF67_09170"
     CDS             1331..2170
                     /locus_tag="PLF67_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE90220.1"
                     /translation="MKQTLVRRHQFQTLRRLLLNIPLMVVILIPLLYTLSISVMQPDE
                     IYTNRLIPSSVSFENYVRALTGSYYNFPRMILNSFIVSTSVMLGQMVTCSLAAFAFSF
                     LEFKGKRVLFLAVLATMMVPGEVTIIANYLTMLRWGWLDSYRALTFPFLTSAMGIFLL
                     RQYYLTFAKELYEAAKLDGCSNIRFMGSIVVPLSRPALGALGAYVFLNTWNQYMWPLL
                     VTNSRHYRTVQVGISMLYDIDAYSLGLMMAGVIIVIVPSLSIFVFMNKQLIHGLMAGA
                     VKG"
     gene            2250..3584
                     /locus_tag="PLF67_09175"
     CDS             2250..3584
                     /locus_tag="PLF67_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE90221.1"
                     /translation="MKRTLSLFLVVLLLAGSAFAAGKTEAVKAEGPVELTFWHAMGGV
                     NGEAIAHMVDQFNKQYEGKIHVTYEFQGTYDDEFAKIQASGGNYPCDVMQVYDIGTRY
                     LIDSGWTLPVQKYIDKDKYDISQIEPNIAAYYTVEGTLHSMPFNSSTPLLYYNKTAFD
                     EAGIKKVPTSFEEIYEVAAKLKQYNPDGSVKRYGFGMGNYGWFFEQWIGKQGLHYVNN
                     NNGRGDAYATAVDFDRNGGGVKIMRVWDKVLKEDIAPYLNRGNNDAKAAFIAGNIAIT
                     LESTAALKSLLLNVGDKFEIGVGYFPKIDKNDVGGVSVGGGSLWMMNTGDTRREDASW
                     EFLKFMISPDMQAYWNMMTGYFPITVKAAETDVFKQNIAKYPQFQVAIDQLHSSAPQY
                     AGALLSVFSESRKIVEDYTERLANREGTPESAVEGMARDINRAITNYNLVNY"
     gene            3736..>4057
                     /locus_tag="PLF67_09180"
     CDS             3736..>4057
                     /locus_tag="PLF67_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOE90222.1"
                     /translation="MLAPAEHARLTEFAKEIRKKTLYTIGNLGVGHIGGALSIAEVLA
                     LLYGKVMRIDPNNPQWEDRDFFILSKGHAGPALYATLALKGYFPLSELDTLNRGGTNL
                     PSHCD"
BASE COUNT          962 a         1099 c         1017 g          979 t
ORIGIN      
        1 atgaccgtcc ttggagagct gcacatccaa ttcgatccca tcggcgttcg cctccaacgc
       61 cttggcaaac gcgagcatcg tgttctccgg atatgcaccg ctgtatcccc ggtgcgcata
      121 aatcttcatt gtacctctcc cctttgcttc accttcatat tcttctggcg gctcaaagca
      181 tgcgatttaa aatttaggtt aaatctttat tatatttaac taattttaac cactattctt
      241 caacaccaaa ttctgaggtt agtatagcta atccattatt aaaaatcaac ttatatcaag
      301 cttaattttg tacttgtact ctctcctttg aaccagtata cttcatgtat gggaatgttt
      361 gcaaaggttt attttaaccc gtgttttaat tgctgaaaac ggcccatttt tggaggatgt
      421 ctatgagagc aatgcagagg aagggcgatg ctccgaaagt ctggaaaggg tttcccggtg
      481 ccgttgaacc ttacctcttt ctcgtcccct cgttcattat tttcggatta tttcttttct
      541 atcctttttt caggacgatc tatttgagcg ctttcctgac gaacaagacc gggcaaccga
      601 agattttcgt actcttcacc gacatggaca actacctcgc ccttttccgg gatccctcat
      661 tctggaaaag cgtcggcgtc acgttccagt ttgtattcct ggtcgccttt ggcggattgc
      721 tggtcggact gaccaccagt ctccttacgg aaaaaaagtt tcccggatcc gatgcggcca
      781 ccgctgtcta cgcgatgcca atcgcaatcg catccgctgc ggcttccatg gcgttcaaga
      841 tgatcctcca tccgacaaac ggactgatca acaatgtgct ggggacgcag atcaacttca
      901 ccggggatgt ccggtatgcc ctcgcttccg tcgtcggggt gacgatctgg ctgaccagcg
      961 ggatcaactt catcttcatc agcgccggct tgcgaaacgt gcccgccgaa ctgtatgaca
     1021 gcgcctccgt cgacggcgcc gggtattggc ggaaggtctg gaatgtgacg ataccgacga
     1081 tctcgcccac cctctttttc cagatcatca tcaacatcat cggcgcgttc caatcgttca
     1141 gccagatcaa gctgctcacc gaaggaggtc cttccaatac cacgaacgtc atggtctggt
     1201 cgatctaccg ggacgcgttc ttcaacttcc gcttcggttc cgccgccgcc cgatcggtca
     1261 ttctcttcct catcgtgttg ggaatcacga tcatccagtt ccggtttgag aagaaaggag
     1321 ttaactactg atgaaacaaa cattggttcg ccgccatcaa ttccagaccc tccgaagatt
     1381 gctgctcaac attccgctga tggtggtcat cttgatacct ctgctctata cgctttccat
     1441 ctcggtgatg cagccggatg aaatctatac caaccggctg attcccagtt cggtgagttt
     1501 tgaaaactac gttcgggcat tgaccgggtc gtactacaac tttccgcgga tgatactcaa
     1561 ctcgttcatc gtctcgacgt ccgtcatgct tggccagatg gtcacatgca gcctggccgc
     1621 gttcgccttc tcctttctgg agttcaaagg gaagcgggtg ctctttttgg cggtgctcgc
     1681 cacgatgatg gttcccggcg aggtgacgat catcgcaaac tacctgacga tgctccgctg
     1741 gggatggctg gactcctaca gggctctcac cttccccttc ctaaccagcg cgatgggaat
     1801 cttcctgctg aggcagtact atctgacatt tgcaaaagag ctgtatgaag cggcgaagct
     1861 tgacggttgc agcaacattc ggtttatggg tagcatcgtc gttcccctct cccgtccggc
     1921 gcttggggcg ctgggcgcgt acgtgttcct caacacctgg aaccagtaca tgtggccgct
     1981 gctcgttacg aattcacgcc actaccggac ggttcaggtg ggaatctcga tgctgtatga
     2041 catcgatgcg tattcgcttg gattgatgat ggcgggagtg atcatcgtca tcgtcccctc
     2101 gttgagcatc ttcgtgttca tgaacaagca attgattcat ggattgatgg ccggagcagt
     2161 taagggttga cgggaaaatc catcataccc ttatccgata tagacagatg aaatggattc
     2221 catttcaaga aaccgaaaag gagaaaaaaa tgaaaagaac actcagtttg tttctcgtgg
     2281 tgctcttgct cgcaggttcg gcctttgccg caggcaaaac tgaagccgta aaagcggaag
     2341 gtccggtcga actgaccttc tggcatgcga tgggcggggt caacggcgaa gcaatcgccc
     2401 acatggttga ccagttcaac aagcagtacg agggaaagat ccacgtcacg tatgagttcc
     2461 agggcaccta tgatgatgag tttgccaaga ttcaggcatc cggcggaaac tacccctgtg
     2521 acgtcatgca ggtctatgat atcggaaccc gatatctgat cgactcgggc tggaccctcc
     2581 cggtacaaaa atacatcgac aaggacaagt acgacatcag ccagatcgag ccgaacatcg
     2641 ctgcgtacta tacggtcgaa ggaaccctcc attcaatgcc gttcaacagt tccaccccgc
     2701 tcttgtacta caacaagacc gcatttgacg aagccggcat caagaaggtt cccacgagct
     2761 ttgaggagat ctacgaagtc gctgccaagc tgaagcagta caatcccgac gggtcggtga
     2821 agcgctacgg cttcggcatg ggcaactatg gctggttctt cgagcaatgg atcggaaagc
     2881 agggcctgca ctatgtcaac aacaacaacg gacggggcga tgcatatgcg accgctgtcg
     2941 actttgacag aaacggcggc ggcgtgaaga tcatgcgcgt ctgggacaag gtgctcaaag
     3001 aggatatcgc tccctatctg aaccggggca acaacgatgc gaaagccgcg ttcatcgccg
     3061 gcaatatcgc gatcaccctc gagtccaccg ccgcattgaa atccctgttg ctgaacgtcg
     3121 gcgacaagtt cgagatcggc gtcggatact tcccgaagat cgacaagaac gatgtcggtg
     3181 gcgtttccgt cggtggtggc tcgctgtgga tgatgaacac cggagatact cgccgcgaag
     3241 atgcttcgtg ggaattcctg aagttcatga tctctcctga catgcaggca tattggaata
     3301 tgatgactgg atatttcccg atcacggtga aagccgccga gacggatgtg ttcaagcaga
     3361 atatcgccaa atatccccag ttccaggtcg cgatcgacca gcttcatagc tcggctcccc
     3421 agtacgccgg tgctctcctc agcgtcttca gcgaatctcg gaaaatcgtc gaggattaca
     3481 ccgagcgcct ggcgaaccgt gaaggtaccc ccgaaagcgc tgtcgaagga atggcgcgcg
     3541 acatcaaccg cgccatcaca aactacaacc tggtcaacta ctgatcaata ggcgcaatca
     3601 agaaaaggcc gtcagatatg gcggtctttt ctttttgaaa atccttaaca cgctttggaa
     3661 aaaaagctat actgcactgg aacataactc gatatatcta aaaagattga tacgttgaaa
     3721 ccacaggggg gagagatgct tgcacctgct gaacacgcga ggctcacgga gtttgcgaaa
     3781 gagatacgga aaaagaccct gtacacgatc gggaacctgg gggtcggtca tatcggagga
     3841 gcgctttcga ttgcggaagt gctggccctg ctctacggaa aggtgatgag gatcgatccg
     3901 aacaatcctc aatgggaaga ccgggatttc ttcatcctct caaaaggaca tgccggcccc
     3961 gccctgtatg ccaccttggc gctgaaaggg tatttccccc tttccgaact cgatacgctc
     4021 aaccggggag gcacgaacct gcccagccat tgcgacc
//
LOCUS       DAOSLI010000104         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_19554_length_4046_cov_18.658231, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000104 DAOSLI010000000
VERSION     DAOSLI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19554_length_4046_cov_18.658231"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1285
                     /locus_tag="PLF67_09185"
     CDS             119..1285
                     /locus_tag="PLF67_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE90223.1"
                     /translation="MLKVRKNDLPLLYTVYFSFFTSGMMSTLIGSLLPSMKSEYELSY
                     VLGGVVLSAHQIGNFTALILAGFLPYLLGRKKSTLVLSSGIIIGFTMMTLTGNPLTLI
                     IAFVLTGVGRGTRSNITNVLISEITENKTAGLNLLHASFAIGAFLAPLVALLTTLIRG
                     VHWRLAAYLLIVLELFVLLAIGRSTISNTPTRREKGTRPDFLTDSSYWINTGILFFYL
                     CTEASVIGWLVTYFRDTGRLSASLSLTTSTALWVFILFGRIFCAFLSTRMNKNILLVI
                     LGSLQLLFFLLMIGIRSIVLTYISLFGFGLAMSGTYPTTLSTMDPRYNASTIVTGTTI
                     ATASVGAITMPIIVGSVAEKAGIGSGLATISITIVTMLVLMVVKLLRERPATEG"
     gene            complement(1272..2021)
                     /locus_tag="PLF67_09190"
     CDS             complement(1272..2021)
                     /locus_tag="PLF67_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOE90224.1"
                     /translation="MKLISWNINGLRAIQQKGFNDYFREADADIFCLQETKLQEHQVT
                     LETPGYYQYWSFAEKKGYSGTALFTKTEPLSVEYGIGHEFDNEGRVINAEFEEFYVMT
                     VYTPNSQEELARLDRRMEWDDAFREYVKALAEHKGVIICGDLNVAHQAIDLKNPKTNE
                     RNAGYTIEEREKFTRLLEAGFVDTFRYLYPDATEAYSWWSYRFQSRSRNTGWRIDYWL
                     VSERLKERVVDSTIATEVMGSDHAPVILTLR"
     gene            complement(2032..3066)
                     /gene="asnA"
                     /locus_tag="PLF67_09195"
     CDS             complement(2032..3066)
                     /gene="asnA"
                     /locus_tag="PLF67_09195"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOE90225.1"
                     /translation="MAKYFPDGYIPLMERKQTEKAIKYVKDTFERELSGGLMLSRVTS
                     PLFVPRGSGINDDLNGVERPVVFQIGNFDNKDMEIVQSLAKWKRLALATLGFKPGTGL
                     YTDMNAIRPDDDLDAIHSIYVDQWDWELVITEEQRSLDFLKFVVSKIYSAMRRTEFLV
                     CEMFPVIKPTLPEKITFIHAEEALRRYPNLSVYDREYELAKEYGAVFIIGIGAKLSDG
                     TSHGGRAPDYDDWSTPNSDGYLGLNGDIVVWDPIRKNLMEITSMGIRVNPESLIRQLT
                     IRGMEERANLYWHKRLLANEMPLTIGGGIGQSRLCMYFLKKAHIGEVQASIWPQDVME
                     EAASLGVVLM"
     gene            3529..>4046
                     /locus_tag="PLF67_09200"
     CDS             3529..>4046
                     /locus_tag="PLF67_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOE90226.1"
                     /translation="MDFPNDFLWGCATASYQVEGATEKDGRTPSIWDTFAKKPGAVEA
                     GNDGSVACDQYHRYEEDVELIASLGFEAYRFSIAWPRIIPDGIGAVNPKGVDYYVRLC
                     KALHAKGIKAVATIYHWDLPQSLQDAGGWPERRTAYAFAEYAKACFTELGPYVDQWIT
                     LNEPWCSSYLSYL"
BASE COUNT          945 a         1055 c          966 g         1080 t
ORIGIN      
        1 actgccaata ggtgggagag taggtagctg ccgggcctct tcttttgtat cttccgcatc
       61 cctctacgca tgggggattt ctttatgtgc aggagcattc atggaccctt tatccaacat
      121 gttgaaggta cggaagaacg atcttcccct cctctatacg gtatattttt cgtttttcac
      181 cagtggaatg atgtctaccc tcattggttc actgcttccc tctatgaaga gtgaatacga
      241 gctcagttac gtccttggcg gtgtggttct cagcgctcat cagatcggta actttaccgc
      301 cctcattctg gccgggtttc tgccctacct tttgggaaga aagaagagca cgcttgttct
      361 ctcctcgggg atcatcatcg gttttacgat gatgacgctt accggcaacc cgctcactct
      421 catcatcgcg ttcgttttaa ccggtgtcgg acgggggacg cgaagcaata tcaccaatgt
      481 cctcatcagc gaaattactg aaaacaagac agccgggttg aatctgcttc atgcctcgtt
      541 tgcaattgga gcgttcctgg cgccccttgt cgcacttctg acgacattga tcaggggggt
      601 tcattggcgg cttgccgcat atctcctgat tgttttggaa ctgttcgttc ttcttgcgat
      661 tggccggtcg acgatcagca atactccgac ccggcgagag aaaggaacaa gaccggattt
      721 ccttaccgat tcttcctact ggatcaatac ggggattctg ttcttctacc tttgtaccga
      781 ggcttcggtg attggctggc tggtcaccta tttccgagat accggccgtc ttagtgcgag
      841 tctttcattg accacaagca ctgcgctctg ggtgttcatt cttttcgggc ggatattttg
      901 cgcgttcctt tcaacgagaa tgaacaagaa tatcctcttg gtcattctcg gatcgctgca
      961 actcctcttt ttcctgttga tgatcggcat ccgaagcatc gtgctgacat atatcagtct
     1021 gttcggtttc ggtctggcga tgagcgggac ctatccgacc actctttcaa cgatggatcc
     1081 acgctacaat gcatcgacga tcgtaacggg cacgacgatt gcgactgcat cggtcggggc
     1141 gatcacgatg cctataatcg tgggttcggt cgccgagaaa gcgggaatag gttcggggct
     1201 tgccacgatc tccataacga tcgttacgat gcttgtgctg atggtggtga agctcctcag
     1261 ggaacgaccg gctaccgaag ggtaagaatc accggcgcat ggtcgctacc catcacctcg
     1321 gtggcgatcg tcgaatcgac cacccgttct ttcaagcgtt cgctcaccag ccaataatcg
     1381 attctccacc cggtattccg tgatcggctt tgaaagcgat acgaccacca cgaataggct
     1441 tcggttgcat ccggatacag atagcggaag gtgtcgacaa atccggcttc cagcagccgg
     1501 gtgaacttct cccgttcttc gatcgtatag ccggcgttgc gttcattcgt tttcgggttt
     1561 ttcaagtcga tcgcctgatg ggctacattc aaatcgccgc agatgatgac gcctttgtgt
     1621 tccgccaacg ctttgacata ttctcgaaac gcatcatccc actccatccg ccgatccaag
     1681 cgggcaagct cttcctggct gtttggagta tagaccgtca tcacgtagaa ctcttcgaac
     1741 tcggcgttga tgactcgccc ttcattgtcg aactcatgtc cgattccata ttcgacggaa
     1801 aggggttccg tcttggtgaa caacgcagtt ccgctatagc ctttcttctc ggcaaatgac
     1861 caatactgat aatagccggg ggtttccaga gttacctggt gttcctgaag cttcgtctcc
     1921 tgcaggcaga agatatccgc atccgcctcc ctgaaatagt cgttgaagcc tttctgctgg
     1981 atggctctca atccgtttat gttccaactt atcagtttca ctgctttctt cctacatcaa
     2041 tacgacgcca aggcttgcgg cttcttccat cacatcctgc ggccaaattg atgcttgcac
     2101 ctctccgata tgggctttct tcaggaaata catgcagagg cggctttgac cgattccgcc
     2161 tccgatcgta agcggcatct cattggccaa aagccgtttg tgccaataga ggtttgcccg
     2221 ttcttccatt ccacgaatcg tcaattgccg gatcaatgac tccggattca ctcgaattcc
     2281 catcgacgtg atttccataa gattctttcg aataggatcc catacgacga tgtcgccgtt
     2341 gagaccgaga tacccatcgc tgttcggcgt cgaccagtca tcatagtcgg gggctcgacc
     2401 gccatgagat gttccgtcgc tcagcttggc accgatgccg atgatgaaga ccgccccata
     2461 ttccttggcg agttcatact cgcgatcata cacgctcaga ttcgggtatc gtctcagcgc
     2521 ctcctcggca tggatgaagg tgatcttctc gggaagcgtc ggcttgatta ccggaaacat
     2581 ttcacacacc agaaactcgg tacgtctcat cgccgaatag atcttggaaa ccacgaactt
     2641 caaaaagtca agggaacgct gttcttccgt gatgacaagc tcccaatccc attggtcaac
     2701 gtagatcgag tggatagcgt ctaaatcatc atcagggcga atggcattca tatcggtgta
     2761 caaaccggtt ccgggcttga acccaagggt ggcaagcgcc agacgcttcc atttcgccaa
     2821 ggattggacg atctccatat ccttattgtc gaagttccca atctgaaaga ccaccgggcg
     2881 ctcgacgcca ttcaggtcgt cattgattcc cgagcctcgg ggaacaaaca acggactggt
     2941 gacgcggctg agcatcaacc cgccggaaag ctcccgctca aaggtgtctt tcacatattt
     3001 gatcgctttc tcggtctgtt tccgctccat caacgggatg tatccatcag gaaagtactt
     3061 cgccatcttg catttctcct ataatcgcct agcatatcgt attttctcct gcagtgaaag
     3121 ctacctgctt gaacatcgga gattccaata ccatgctcat gatccataga aaagttgttt
     3181 actccgaatg agaaactgaa tcatactaaa caggtttaga ttggaaccct tataaaacga
     3241 tatgattaca acatgtaaag aaattaaaaa taattgtaat agtatttaaa taatatagaa
     3301 tatatataat aaaaatgtat atgattgcat ataaatatat atgatgagat tcaagcaatt
     3361 tgtcaggtaa tcgttgccgt tgccaatcct gcgtgatatg atggtttgga agtttagcag
     3421 aggcagactc cgcatgaaga tcaaacaccc aaaaagtatg tcgccctact gcccataata
     3481 ggtataaacc tgagcaacgt caggatcatg tatacgtagg aggtatctat ggatttcccc
     3541 aatgattttc tctggggttg tgctacggca agttatcaag tggaaggcgc caccgaaaaa
     3601 gacgggcgaa caccctcgat ttgggacacg tttgcaaaga aacccggtgc ggttgaagca
     3661 ggcaacgacg gatcggtagc ctgtgaccag taccatcgat atgaagaaga tgtagagctg
     3721 atagcgagtc ttggctttga ggcctatcgt ttttcgatcg cttggccaag aatcattcct
     3781 gacggtatcg gagcggtcaa tccgaaagga gtcgattatt atgtccgtct ctgcaaggct
     3841 cttcatgcga aaggaatcaa agcggtcgca acgatctatc attgggatct tcctcaaagc
     3901 ctccaggatg ccggcggctg gcccgaacga agaaccgcct atgcgttcgc cgagtatgca
     3961 aaagcctgtt ttaccgagct ggggccttac gtagaccaat ggatcacgtt gaacgagccg
     4021 tggtgctcct cgtatctctc ctatct
//
LOCUS       DAOSLI010000105         3888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_20594_length_3888_cov_15.344639, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000105 DAOSLI010000000
VERSION     DAOSLI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20594_length_3888_cov_15.344639"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..973
                     /locus_tag="PLF67_09205"
     CDS             77..973
                     /locus_tag="PLF67_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE90227.1"
                     /translation="MTHKNHARKRTDGMVALAFLLPLLAGLGVFYFYAFVKNIYYSFT
                     NLGSFGKATFTGLENYLRLFSDPKFYRSLGNTLRYVVIAVPAVVVLSTLNAYLLSRLR
                     ILSGVFRTLLFLPAVTMPAAIGILWRWLFNYKFGLINALLGKFGIEPVAWLSDPAVSL
                     YSVSIVLVWALLATQTVIILTGLQNIPAMYEEAALIDGANRRQIFLKITLPLLSPTLF
                     FVITITTINVFQVFDFIFLMLRPATLAIQYASSLVYYFYDRAFIAYEKGYGVAVSMIL
                     FLIILLVTVIQFIFQKKWVHYS"
     gene            970..1791
                     /locus_tag="PLF67_09210"
     CDS             970..1791
                     /locus_tag="PLF67_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE90228.1"
                     /translation="MKKDKHTPVIMILLIIASAMTLLPYVWMVLTSLKTYEEAIRIPP
                     KILPEKFQWINYQIIWNKFPFATLYLNTFFVVIATVAGNIVVAAMAAYAFARLKFFGR
                     DLLFIAILGLLMIPGQIFLVPHYQIINSWGLSNTRIALVLPGIFRVFGVFFLRQAFLT
                     IPREMDEAALVDGANHLQIFVKVLIPLISSSIVSLGILIALWTWTELMWPLIINRSLD
                     KLTLSAGLAFLIGEHTTYYEQVMAGGVISSLPVIILFFFFQRYIIEGIAATGVKG"
     gene            1801..2475
                     /locus_tag="PLF67_09215"
     CDS             1801..2475
                     /locus_tag="PLF67_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOE90229.1"
                     /translation="MLDFLVVTAHPDDESAIAGFLLSAKKQGYKTGIICLTKGESGGF
                     ATKEERVLEFEEATALLGFDWVRILDFPDAGIEVSSSAVETLIPLFRETEAKTIITLH
                     PEDYHPDHRAVAALVDRAVFVAGLKKHSTDDTTWHPAQVLSISLDARRATRRADIVYD
                     ISDVQEEWRAFLKCYPSQKIAQFVEIRARYYGLLGGYPLAEGFFLNQPLRLDGPGTLI
                     HKGKRN"
     gene            2512..3768
                     /locus_tag="PLF67_09220"
     CDS             2512..3768
                     /locus_tag="PLF67_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOE90230.1"
                     /translation="MKKRMIVLVLLIVVAGILFAAGAKEAGKSKLTLALWDENQKPAI
                     QKIVDQYNASQDKVEVVIELTPWDTYWTKLDAAAGSKSAPDIFWMNVYMPKYVDGGVL
                     LPLDDLIARDKVDMNQYVPATVKMNRFEGKTYGMPKGLDSVAVALNLELFEKYGVATP
                     KEGWTWQDMRVLAAQLRDAIAKKGGDEYPIVMELDAQPSHFNFAYQTGGYVISDDFKK
                     SGYNLPETTLAYQRVVDLMDDGLMAPYVVLSDTKGTDLFLSGKGAILFVGSWKSSVLE
                     NSSLGVNKKIRLITMPNQEAGNTSVLGGLGYVISAFTKDKEGAWDAVKFITGEVGNRI
                     QAEEGIDIPALKKSQATYLANFKNINIDAFFKAAEHAVPFPAGPDLVLWIGIIGDRAA
                     EIFGGKTTAAAGTAAIYKEMQAILNK"
BASE COUNT          843 a         1038 c         1093 g          914 t
ORIGIN      
        1 tcgatgaccg cgtccgtcaa ggaatccctt tccccccgaa tcagacccga gctcgctgag
       61 gcgatggaga aatactatga ctcataagaa ccatgcacga aaaagaactg acgggatggt
      121 agccctggcg tttcttctgc cccttttggc gggcttggga gtcttctatt tctatgcctt
      181 cgtgaaaaac atctattaca gtttcacgaa tctcggatcc ttcggaaaag cgacgtttac
      241 cggtctggaa aactacctgc ggcttttcag cgatccgaag ttctaccgat cccttggaaa
      301 caccctgcgg tatgtggtca tcgccgtccc ggcggtcgtg gtcctttcca ccctgaatgc
      361 atatctgctc tccaggcttc ggatattgag cggagtcttc agaaccctcc tgtttctgcc
      421 ggcggtcacg atgccggcgg cgatcggaat tctctggcgc tggctgttca actacaagtt
      481 cgggctcatc aacgccctct tggggaagtt cgggatcgaa ccggtcgcct ggttgagcga
      541 tcccgcagtc tcgctgtatt cggtgagcat cgtcctggta tgggcgctgc ttgcgaccca
      601 gacggtcatc attctgacgg gactgcagaa cattccggcg atgtatgagg aagccgccct
      661 gatcgacgga gcgaacaggc ggcagatctt tttgaagatc accctgcctc tgctttcgcc
      721 caccttgttt ttcgtgatca cgatcacgac gatcaacgtc ttccaggtct tcgacttcat
      781 ctttttgatg cttcgtccgg cgacgctggc gatccaatat gcctcctcgc tggtctacta
      841 tttttacgat cgggccttca tcgcgtatga aaaagggtac ggagtggccg tttccatgat
      901 tcttttcctc atcattctcc tggtcacggt gattcagttc atcttccaga aaaagtgggt
      961 gcattattca tgaagaaaga caagcatacc ccggtgatca tgattctcct catcatcgct
     1021 tcggcgatga cgctcctgcc atatgtctgg atggttttga cctccctgaa gacctatgaa
     1081 gaggcgatcc ggattccgcc gaagatcctt cccgagaagt tccaatggat caactatcaa
     1141 atcatctgga acaagttccc ctttgctact ctctatctga acacgttttt cgtcgtcatc
     1201 gcgacggtcg cgggaaatat cgtggtcgcc gccatggccg cttacgcgtt cgcccgcctc
     1261 aagttcttcg gccgggatct tctgttcatc gcgattctcg gtctgttgat gattcccggg
     1321 cagatcttcc ttgttcccca ctatcagatc atcaacagct gggggctgtc caacacacgc
     1381 atcgcattgg tgcttccggg aatcttccgg gtgttcgggg tgtttttcct ccgccaagcg
     1441 tttttgacga tccctaggga gatggatgaa gcggctctcg tggatggggc gaaccatctt
     1501 cagatctttg tcaaagtcct cattccgctc atctcctcat cgatcgtgag tcttgggatt
     1561 ctgatcgccc tgtggacgtg gacggagctg atgtggcccc tgatcatcaa ccgttctctg
     1621 gacaagctga ccctttcagc cggcttggcg ttcctgatcg gggaacatac cacatattat
     1681 gagcaggtga tggccggagg agtcatctct tcacttccgg tgatcatcct gttcttcttc
     1741 tttcaacggt acatcatcga aggtatcgca gctacgggag taaagggata ggaggcaatc
     1801 atgcttgatt ttttagtcgt tacggcgcat cccgatgatg agagcgcgat cgcagggttt
     1861 cttctctctg ccaagaaaca gggatacaag acgggaatca tctgtcttac gaaaggggaa
     1921 agcggaggct ttgcgacgaa agaggagcgg gtgctcgagt ttgaggaagc tacggccctg
     1981 ctggggttcg actgggtgcg gattctggac ttccccgatg cggggattga agtatcgagc
     2041 tccgcggtgg agacgctgat ccccctcttt cgggagacgg aggcgaagac gatcatcacc
     2101 ctgcatccgg aagactacca cccggaccac cgggcggtgg ccgctctggt tgatcgggcg
     2161 gtgttcgtgg cggggctgaa gaagcattcc acagacgata cgacatggca tcccgcccag
     2221 gtgttgtcga tttctctcga tgcccgaaga gcaacgagga gggcggatat cgtatacgac
     2281 atttccgatg tacaggagga gtggcgggcg ttcctgaaat gctatccatc tcagaagatc
     2341 gctcaatttg ttgagatcag agcacggtac tacgggctgc tggggggata tccccttgct
     2401 gaagggtttt tcctcaacca gccgctgcgc ctggacggcc cgggcactct gattcacaaa
     2461 gggaaaagaa actaaagccc tcagacagag ggtaatataa taggaggaac tatgaaaaag
     2521 agaatgattg ttttggtgct tctcattgtt gtcgccggca ttctgtttgc cgccggagcg
     2581 aaagaagcgg ggaaaagcaa gcttaccctg gcgttgtggg atgagaacca gaaacccgca
     2641 atccagaaga ttgtcgatca gtacaatgct tctcaggaca aggtggaggt ggtgatcgag
     2701 ctcactccgt gggataccta ttggacgaaa cttgatgccg ccgccggcag caagagcgct
     2761 cccgatatct tctggatgaa cgtctacatg ccgaagtatg tggatggcgg agttctgctt
     2821 cctcttgatg atctgatcgc ccgggacaag gtcgacatga accagtatgt ccctgcaacg
     2881 gttaagatga accggttcga aggcaagaca tacggaatgc ccaagggact ggacagcgtt
     2941 gcggtcgctt tgaacctgga gctgttcgag aagtacggcg tcgccacccc gaaggaaggg
     3001 tggacctggc aggatatgcg cgtgctcgcg gcccagcttc gcgatgcaat cgccaagaag
     3061 ggcggagacg agtatccaat cgtgatggag ctggatgccc agccctccca cttcaacttc
     3121 gcctatcaga ccggaggata tgtgatcagc gatgatttca agaagtccgg atacaacctg
     3181 cccgagacca ccctcgcgta ccagcgggtc gtggatctga tggatgatgg tctgatggct
     3241 ccctacgtcg tcctctccga tacgaaggga accgatctct tcctctccgg caagggagcg
     3301 atcctctttg tcggctcctg gaagagctcc gtcttggaga actcctcgct gggcgtgaac
     3361 aagaagatcc gtctgatcac gatgcccaac caggaggcgg gaaacacctc cgtccttggc
     3421 ggactggggt atgtgatcag cgccttcacg aaggataagg agggcgcctg ggatgccgtg
     3481 aagttcatca ccggagaggt cggcaaccgg attcaggcgg aagagggaat cgacattccc
     3541 gccctcaaga aatctcaggc cacgtacctc gcgaacttca agaacatcaa catcgatgcg
     3601 ttcttcaaag cggccgaaca cgccgtaccg ttcccggcgg gaccggatct tgtcctctgg
     3661 atcggaatca tcggcgaccg tgctgcggag attttcggtg ggaagacgac cgcagcggct
     3721 ggaactgcag caatctacaa ggagatgcag gcgattttga acaagtaggt aagatgatta
     3781 ccgaacatga cagggggctc cggccccctg ttcctcgttc gatcagaaga agtgatccga
     3841 agtgaagatc agcgtggact ggccgtcttc gtcataccag aggcggaa
//
LOCUS       DAOSLI010000106         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_21008_length_3824_cov_11.069514, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000106 DAOSLI010000000
VERSION     DAOSLI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21008_length_3824_cov_11.069514"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLF67_09225"
     CDS             complement(<1..340)
                     /locus_tag="PLF67_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE90231.1"
                     /translation="MTTFEQFLEKLYSDEDRKFYPDEDQMRAINADVNCVVSAGAGSG
                     KTMVLSYRFVRLVLERKARFDQILTLTFTRKAAREMHRRIHDHLSLCNDDDEIQAQLA
                     SFSSAPISTID"
     gene            complement(337..>1146)
                     /locus_tag="PLF67_09230"
     CDS             complement(337..>1146)
                     /locus_tag="PLF67_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOE90232.1"
                     /translation="LSATKIDAFAKCPYIYLASYLYKIEPFDYRETLINNKAIGVLLH
                     DAYERFFSSIGPFDSGKIESYEIGLQTAFDHALLKTYGTKGPTASIRQWIIHTFRPKI
                     LKILEEEKKYFEHLGTTDTEKELAWKGDRVILDGRIDRIIGIDPKKKEWGVIDFKKGK
                     AFDPVKYKNDEQVEHVEFYQLLVYQALIERNDLGKAMVAAYYSVEDGKYKFLYEQKDA
                     KKQELCRIELDRMLERILSEVQRGDYAATPGDKACRQCSFRALCRRRYSAP"
     assembly_gap    1147..1246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1247..2814)
                     /locus_tag="PLF67_09235"
     CDS             complement(<1247..2814)
                     /locus_tag="PLF67_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90233.1"
                     /translation="MSILGDHLASNAVCVLPTEAARRSHLIEYALTSEDGVIAGDSVL
                     SFDTFRSSFLPAYADRLPSNTLIRFIFATEFVESGNSISSFYNPDFPESKHQALGVIA
                     SMLPSLLKVLTSEIGTLLPRELFRQLQAVHAAYTEFLDRNNLFEPQYDSVSVPSGWNT
                     EREVRILYSDLESEAAVLYEELGRPSWLKLIPTPKADTPTVDVYANHLQEIRSTLRRI
                     RDLLDEGVATHEIMICLAGSDELLATLEDEAYFYGIPLAVRQGRSPLKYPAGRFFSLM
                     NEVHSDRFTLTSLKKLLLEPAIPWKAEERKHIEEFIRLCLKDTILYGSEARPDYFELR
                     LSNRSLRRWYVTFRDALTAIVQATTVADLVRSLSFFRKRFWEEEEWHGTPQEEVFAHA
                     MNQIEQLDRALKACNRERLDGLYALFLSHLQWTQYVPQQQDEGIGVYRWPQGASITGP
                     YRFFLGLDQEGAEVVDNPFPFLSERVRQTLMKRKELGRYHLQAASLGNSTLSCHRRSN
                     NGEKLIHFFFLEEGT"
     gene            2846..3682
                     /locus_tag="PLF67_09240"
     CDS             2846..3682
                     /locus_tag="PLF67_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOE90234.1"
                     /translation="MEQKNRHLLFTLITGLLFFLGVGVYATSLVHPYISAYLAANIPF
                     LALALGLILSHSAVLKEPLIGLVTETNAWSWRLALLAAGSYLAVTALFTVISALRYPQ
                     DYHALRIPLLKRLGFIATALIITPIQTTGEEILFRIIPLKLMKKIQSKAIKALVPTLI
                     FTLLHLANREIAQSQNRLAVLLYYALFGFIPSYIAVRSDRYEFPLAIHAANNLFVVLF
                     CNYEHSSLPSLPLFTSTRPVGTWMDILQLTAALAVSYSTIPQRGMESNESSVEKTTSL
                     DA"
     gene            complement(3607..>3824)
                     /locus_tag="PLF67_09245"
     CDS             complement(3607..>3824)
                     /locus_tag="PLF67_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOE90235.1"
                     /translation="VIPVASFLICRMIGIDPVITGAGVILAAMPGGSTTVILASKYGS
                     DTAYASKLVVFSTLLSLLSIPLWGMVL"
BASE COUNT          807 a         1046 c          992 g          879 t
ORIGIN      
        1 cgtcgatcgt gctgatcggg gcgctggaaa aggaagcgag ttgggcttgg atctcatcat
       61 catcgttgca gagcgagagg tggtcgtgga tccggcggtg catctcccgg gcggctttgc
      121 gggtgaaggt caaggtgagg atctgatcaa agcgcgcttt gcgttccaga acgagccgca
      181 cgaagcgata gcttaggacc atcgtcttcc cgcttcccgc tccggcgctc actacgcagt
      241 tcacatcggc gttgatcgcc ctcatctggt cttcatcggg ataaaatttc cggtcttcgt
      301 cggaatacaa tttttcaaga aattgctcga aggtggtcat ggcgccgagt acctccttcg
      361 gcacagtgcc cggaaagaac attgcctgca agccttgtca ccgggggtgg cggcgtagtc
      421 acccctttgg acttcagata ggattcgctc cagcatcctg tccagttcga ttcggcagag
      481 ctcctgcttt ttcgcatcct tctgttcgta caggaatttg tatttaccgt cttcaacgga
      541 gtagtaggcg gctaccatcg ctttaccgag atcgtttcgt tcgataaggg cctgatagac
      601 caacagctga tagaattcga cgtgttcgac ttgttcatcg tttttatatt tgaccgggtc
      661 gaacgcttta ccctttttaa agtcaatgac tccccactcc ttcttttttg gatcgatccc
      721 gatgatccgg tcgatccgcc cgtccaggat cacccggtct cctttccatg caagctcctt
      781 ctccgtgtcg gtggttccga ggtgttcaaa atatttcttt tcctcctcca aaatcttgag
      841 gatcttggga cggaaggtat ggatgatcca ctggcggatc gaagcggtgg gtcccttggt
      901 tccgtacgtc ttcagcagtg catggtcgaa cgctgtttga agaccgatct cataggattc
      961 gatcttcccg ctgtcgaagg gcccgattga ggagaaaaac ctttcatagg catcgtgcag
     1021 cagcactccg attgccttgt tgttgatcag ggtttcccgg tagtcgaacg gctcgatctt
     1081 gtacaggtag ctcgccagat agatgtacgg gcacttggcg aaggcatcga ttttcgtggc
     1141 ggagagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngttc cctcttcgag
     1261 gaaaaagaag tggatgagct tctccccatt gttggagcgg cggtggcagg acagggtgct
     1321 gttacccaag gaagccgctt ggaggtgata tcgtccgagt tccttccgct tcatcagggt
     1381 ttgtcgaacc cgttcggaaa ggaaggggaa cggattatcg accacttccg ctccctcttg
     1441 gtcgagtcca aggaaaaagc gatatggacc ggtaatgctc gccccttgcg gccagcgata
     1501 gaccccgatt ccttcatcct gctgctgggg aacgtactgc gtccactgca ggtgggagag
     1561 aaagagggcg tacagaccgt cgagacgctc acggttgcac gctttgaggg cccggtcaag
     1621 ctgttcgatc tggttcatgg catgggcgaa cacctcctcc tgaggggttc catgccattc
     1681 ctcttcttcc cagaatcgct tgcgaaagaa ggaaagcgaa cgaaccagat cggcgaccgt
     1741 agttgcctga acgatggcgg tgagcgcatc gcggaacgtc acataccatc ttctcaagga
     1801 acgattgctc aggcgcaatt cgaagtagtc ggggcgggct tccgatccat agaggatcgt
     1861 atccttcagg cagaggcgga tgaattcctc aatgtgtttt cgctcttccg ctttccaagg
     1921 gatggccggt tccaagagga gcttcttcag gctcgtcagg gtaaaccgat cgctgtgcac
     1981 ttcgttcatc aaggagaaga agcgaccggc gggatacttc agcggtgaac gaccctgacg
     2041 gaccgcaagc ggaatgccgt agaagtatgc ctcatcctcc agcgtcgcaa gcagctcatc
     2101 gctgcctgcc agacagatca tgatctcatg cgtcgccacc ccctcatcga gcaggtctcg
     2161 aatccggcgg agggttgaac gaatctcctg aagatggttc gcatacacgt caaccgtagg
     2221 ggtatccgcc ttgggcgtcg ggatcaactt cagccagctt ggcctgccca gctcctcata
     2281 caggacggca gcttcactct ccagatcgct gtagaggatt cgaacctccc gctccgtgtt
     2341 ccaaccgctc ggaacggata ccgaatcata ttggggttca aacaggttgt ttcgatcgag
     2401 gaactccgtg tatgcggcat gcaccgcctg aagttgtcga aacagctccc ttggcaggag
     2461 ggttccgatc tccgaagtga gcaccttcag gagggaggga agcattgaag cgatgacgcc
     2521 caaggcctga tgctttgatt cgggaaaatc gggattgtaa aaggaggaaa tcgagttgcc
     2581 gctttcaacg aactccgtgg cgaagatgaa acgaatcaat gtatttgagg gaagccggtc
     2641 ggcataggcc ggtaaaaatg aggagcgaaa cgtatcaaag ctcagaacgg aatcgccggc
     2701 gatcaccccg tcttcactcg ttaaagcata ctcaatgagg tggcttcgcc ttgccgcttc
     2761 agtaggaagg acgcacactg cgtttgatgc aagatgatca ccaagaatag acatcccctg
     2821 cagtataccg tacatttaca aaacaatgga acaaaaaaac cgccacctgc ttttcaccct
     2881 catcaccggt cttctgttct ttcttggcgt gggagtctat gcgacatccc tggtgcatcc
     2941 ctacatctcg gcgtatcttg ccgcaaacat ccccttcttg gcccttgccc tcggattgat
     3001 tctctcccac tcggctgttc tgaaagaacc gctcatcggt cttgttactg aaacaaacgc
     3061 gtggtcttgg cgccttgccc tcttggcggc gggatcttat ctggcggtga ctgcgctctt
     3121 taccgtcatc agtgcactcc gctatcctca ggactaccac gcgcttcgca tcccgctcct
     3181 gaagcggctg ggcttcatcg ccacagccct catcatcact ccgatccaga ccaccggcga
     3241 ggagatcctc tttcggatca tcccgctgaa actgatgaag aagatccaaa gcaaggcaat
     3301 caaggctctg gttcccaccc ttatctttac acttctccat ttggccaatc gcgaaatagc
     3361 gcaaagccaa aaccggcttg cagtccttct ctactacgct ctgttcggct tcatcccttc
     3421 ctatatcgct gtacgaagcg atcgctacga gttcccgttg gcgatccatg ccgccaacaa
     3481 tctcttcgtc gtcctcttct gcaactatga acactcgtcg cttccatccc tgccgctctt
     3541 caccagcacc cgaccggtcg gcacatggat ggatatcctt cagttgacgg cggcattggc
     3601 ggtcagttac agcaccatcc cccaaagggg aatggagagc aacgagagca gcgtcgaaaa
     3661 gaccacgagc ttggatgcat aggcggtgtc acttccatac tttgatgcga gaatgacggt
     3721 cgtcgatccg cccggcatcg cggcaagaat cacccctgcc ccggtgatga ccggatcgat
     3781 gccgatcatc cggcagatga gaaacgaggc aaccgggatg acca
//
LOCUS       DAOSLI010000107         3796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_21180_length_3796_cov_14.815557, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000107 DAOSLI010000000
VERSION     DAOSLI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21180_length_3796_cov_14.815557"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PLF67_09250"
     CDS             complement(<1..1045)
                     /locus_tag="PLF67_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase (NAD(+)) LigA"
                     /protein_id="HOE90236.1"
                     /translation="MESIEREIMELRSELEAYQRAYYVDSRPLVDDSEYDRKFDRLLE
                     LERRYPEFASSTSPTVRVGSDISADFPEVEHTIAVLSLDKAYTEEELLSWIGKLEKNA
                     SDELGIIVEEKIDGVSIVLYYEDGILDRAVTRGNGVVGNDVSANVKTIPTIPLSLPER
                     ATVAVRGEIYLPKREFETLNKTLETPYANPRNLASGTLRRIKSSEVAKIPLRIFAYEG
                     FWSGDQPATHLEVLAKLKAYGFTINEHFAYFTKDAIDAASALETAGLRAASGSWEEIG
                     SFIHEQAERRASLSYEIDGLVAKVDSIAYRETLGYTGHHPRWALAYKFESPQTVTTLL
                     GIDVQVGRTGRVTP"
     gene            1129..3078
                     /locus_tag="PLF67_09255"
     CDS             1129..3078
                     /locus_tag="PLF67_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOE90237.1"
                     /translation="MAEKRSTMGRLTPLQGVLITSQIARGRFDRIVVPEADPSFVIVA
                     PRDIPGSNRLRVLDAQVPLLSSGTITYRDQPLLALFGSNRESALVKAKGISVIYVEDE
                     PVDSLSVTPYSYTFGNLEEALKEENLNILERSYAFHSKTRKRLTYNRVSVTLDEDVLH
                     IEVPTQWPAHVRESVAEVTGFPKKNIIIHRQPFYAPHDEMLILPAQLAALCSVAAIKS
                     GRNTEILYEMEAAPPRYEITRKTYYQSSGKPQAEEIRVVVDQGCASFFREELVNHLYT
                     GLIPLYPVTALTIHVEVHTTNNRPAHFYGGLGYSEALASSEAHYSELAKLTGYNPLSW
                     RMKFASENPSFSAMIRTDKYAKLKELASTVSKRADFARKNAAYEMQGRMRIRHSTFFK
                     YGRGTGLALAPGIGGFSREYKGTPTQSVQVTLLPNDHVVINTSFYSIGNSAEIWKQIV
                     SDELGVSKKDISFVEDGNELLDSGPSVLNANSGRMPRHILRACQLIKEKRFVEPLPIS
                     ETVTLFRHPSAKDSLFFSNSWVAVVLELEISPLALMPIIRSVWVSITTPKVYEKAAYI
                     SKVRHTIVTTLLEAGAQLSQSRGFAIDIQLEEEGDGMPSSMTAAVKGAVTGALLTALE
                     QSLGSGVETIPVDGPMILNLLRSTH"
     gene            3075..3581
                     /locus_tag="PLF67_09260"
     CDS             3075..3581
                     /locus_tag="PLF67_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOE90238.1"
                     /translation="MKLECTIDSKSLTLHVNSTKPLSLMLIEDVGVKSMLSHCRGDAC
                     GNCIVLVNDVAVLSCIVPAFRLKDAKIRTFETFHRTRLCRDIERAYRTSGNKPCPDCY
                     PSKTLLIESILIELMSSTRSREEGTVDEKAIMQELSLNTCPCMDVAELLEITIQAFEY
                     RRRRRGSN"
     gene            3568..>3796
                     /locus_tag="PLF67_09265"
     CDS             3568..>3796
                     /locus_tag="PLF67_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOE90239.1"
                     /translation="MEATRSPMIHQPRTLNELSLLLQRIEKPLLWAGGTYLMSKSEYY
                     PSPSNRQIIDLAEIEELKRISRTDRYVDIGSM"
BASE COUNT          833 a         1165 c         1035 g          763 t
ORIGIN      
        1 cgggggtcac ccgcccggtg cggcccacct ggacatcgat gcccagaaga gtggtgaccg
       61 tctggggcga ttcgaacttg taggcaagag cccacctcgg gtggtggccg gtgtagccga
      121 gcgtctcccg gtaggcgatc gaatcgactt tcgcgaccag accgtcgatc tcgtaggaaa
      181 gcgaggcgcg gcgctcggcc tgctcgtgga tgaaggagcc gatctcttcc catgagccgg
      241 aagccgccct cagaccggca gtttcaagag cgctcgcggc atcaatcgca tccttggtga
      301 aatacgcaaa atgctcgttg atcgtaaagc cgtacgcctt gagtttagcg aggacttcca
      361 gatgggttgc cggctggtcg ccgctccaga acccttcgta agcgaagatc cgcagcggga
      421 tctttgcaac ttcgctggac tttatccgcc tcaacgttcc gctggcgagg ttgcgggggt
      481 tggcgtacgg ggtttcaagg gtcttgttga gggtttcgaa ctccctcttg ggaagataga
      541 tctccccccg caccgccacc gtcgcccgtt cgggaagcga gagcggaatc gtcggaatcg
      601 ttttcacatt cgcactcaca tcgttgccca ccactccgtt tccccgggtc accgcccgat
      661 cgagaatccc gtcttcgtaa tacaggacga tcgagacccc gtcgatcttc tcctcgacga
      721 tgatgcccag ttcatcggag gcgttctttt caagcttgcc gatccatgaa aggagttcct
      781 cttcggtgta cgctttatcg aggctgagaa cggcgatcgt atgttccact tcgggaaaat
      841 cggcggatat gtcgctgccg acccggacgg tcggagacgt ggaggaagcg aactcggggt
      901 atcgccgctc caattcgagc aggcgatcga acttccgatc gtactccgaa tcatccacca
      961 gcggacggct gtcgacgtag tacgcccgtt gatatgcctc cagctccgac cgaagctcca
     1021 tgatctcgcg ctctatgctt tccatgcctc gattgtatac tcgtcgttca agataggcaa
     1081 ttgccccaat tcttctggcg tggtatcgtt tggaatcaga ggaagaccat ggcggaaaag
     1141 cgatcaacaa tgggcaggct cacccccctt cagggagtcc tgatcaccag tcaaatcgct
     1201 cgggggcggt ttgacaggat cgtcgtcccc gaagcggatc cctcctttgt catcgtcgct
     1261 ccccgggaca tccccggctc caaccgtctg cgcgtcctcg atgcccaggt tcccctgctc
     1321 agcagcggaa cgatcaccta ccgcgaccag ccgctgctcg ccctcttcgg ttccaatcgg
     1381 gagagtgccc tcgtcaaagc caagggaatc tccgtcatct acgtagaaga cgagccggtg
     1441 gactccttgt ccgtgacgcc gtactcctac acgttcggca accttgagga ggctttgaaa
     1501 gaggaaaacc ttaacattct tgaacgttcc tacgccttcc actccaaaac cagaaagcgc
     1561 cttacgtaca accgcgtatc cgtgacgctt gacgaggatg tccttcacat cgaagtgccc
     1621 acccagtggc ccgcccatgt ccgcgagagc gtggcggagg ttaccggttt tcccaaaaag
     1681 aacatcatca tccaccgcca acccttctat gcgccccatg acgagatgct gatcctcccc
     1741 gcccagctcg ccgctctgtg cagcgtggcg gcgataaaaa gtggacggaa cacggaaatc
     1801 ctctatgaga tggaagccgc cccgccgcgc tacgagataa cccgaaaaac ctactaccaa
     1861 agcagcggga aacctcaggc ggaggagatc cgggtcgtcg tcgatcaggg atgcgcctcc
     1921 ttcttccggg aggagctggt aaaccatctc tataccggcc tgatccccct ctacccggtc
     1981 accgcgctga cgattcatgt ggaggttcat accacgaaca accggccggc ccacttctac
     2041 ggggggcttg gctacagcga ggcgttggcc agcagcgaag cccactacag cgagctggcg
     2101 aagttgaccg ggtacaatcc gctttcctgg cggatgaaat tcgcctcgga gaatccgtcg
     2161 ttcagcgcga tgatccgaac ggacaagtac gcgaagctga aagagctcgc ctccacggtg
     2221 agcaaacggg ccgatttcgc ccgaaaaaat gcagcctacg agatgcaggg gcggatgcga
     2281 atccgccact cgacgttctt caagtacggt cggggaaccg gcctcgctct cgctccgggg
     2341 atcggcgggt tcagccggga atacaagggg acgccgacgc aaagcgttca ggtaaccctt
     2401 ctgccgaacg accatgtcgt gatcaacacc tcattctaca gcatcggaaa ttccgccgaa
     2461 atctggaagc agatcgtcag cgatgagctg ggtgtttcaa agaaggacat ctccttcgtc
     2521 gaagacggta atgaattact cgacagcggc ccctcggttc tcaacgcgaa cagtggaagg
     2581 atgccccgcc acatccttcg ggcatgccag ctcatcaagg aaaagcggtt cgtcgagccg
     2641 cttccgatca gcgagaccgt cactctcttc cgccatccct cggcaaaaga ctccctcttc
     2701 ttctccaact cgtgggtcgc cgtcgttctt gaacttgaaa tctcgcccct tgccctgatg
     2761 ccgatcatcc gatccgtctg ggtctccatc accaccccga aggtgtatga aaaagccgcc
     2821 tacatcagca aggttcgcca cacgatcgta acgacgctgc ttgaagccgg agctcagctt
     2881 tcccagtcga gagggtttgc catcgatatc caacttgagg aggaaggtga cggcatgccc
     2941 tcctcgatga ccgccgccgt caaaggggcg gtgaccggag ccctgctcac cgccttggag
     3001 caatcgctcg gatcgggtgt tgaaactatc ccggtggacg gcccgatgat tctgaacctt
     3061 ctaaggagta cccattgaaa ctggaatgca ccattgactc caagtcatta acactgcacg
     3121 tcaattccac caagccgctc tcgttgatgc tcattgagga cgtgggggtc aaatcaatgc
     3181 tcagccactg ccggggcgac gcgtgcggaa actgcatcgt cctggtgaac gacgtggcgg
     3241 tcttaagctg catcgttccg gcgttccgcc tgaaggatgc aaagattcga accttcgaga
     3301 cattccaccg cacccgcctc tgccgggata tcgaacgggc ataccggaca agcggaaaca
     3361 aaccctgtcc cgattgctat ccatcaaaaa cgctgctcat cgaatcgatc ctcatcgaac
     3421 tgatgagcag cacccgtagc cgtgaagagg gtacggtgga tgaaaaagcg atcatgcagg
     3481 agctcagcct gaacacctgt ccctgtatgg acgtggcgga gctgctggag atcacgattc
     3541 aggcgttcga gtataggagg cgaaggcgtg gaagcaacta gatccccaat gatccatcag
     3601 ccacgaactc tgaatgagct ctctcttctc ctgcagcgaa tcgagaagcc ccttctttgg
     3661 gcgggcggaa cctatctgat gagcaagagc gaatactacc cttctccatc caatcgccag
     3721 atcatcgacc tcgccgagat cgaagaactg aagcgaatca gccgaaccga ccgatatgtc
     3781 gatatcggct cgatgg
//
LOCUS       DAOSLI010000108         3590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_22749_length_3590_cov_26.141443, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000108 DAOSLI010000000
VERSION     DAOSLI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22749_length_3590_cov_26.141443"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLF67_09270"
     CDS             <1..333
                     /locus_tag="PLF67_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE90240.1"
                     /translation="SVGKDGNKLIKSQDLYDALEANPKAFKVIDIRGEKDFNEGHVIG
                     SSHTAWAQFGSILPELDKKDKIVIVCFSGQTAGQTVGVLRTMGFDAYSLLGGINNGWK
                     PAGLPLEK"
     gene            368..455
                     /locus_tag="PLF67_09275"
     tRNA            368..455
                     /locus_tag="PLF67_09275"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:401..403,aa:Sec,seq:tca)
     gene            457..1788
                     /gene="selA"
                     /locus_tag="PLF67_09280"
     CDS             457..1788
                     /gene="selA"
                     /locus_tag="PLF67_09280"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOE90241.1"
                     /translation="MYETLPQVEALLQHPSIGKWFVHVSRPVAADVLRITIEEFRKRI
                     RVEEKGFDLDEIVSACEKALATISCQRLQRVINATGVIIHTNLGRAPISRENWERVGL
                     LNYSYTNLEFDVAKGKRGKRSSFTSLLLTKMSGAEDALVVNNNAAAIFLILSAFAKRK
                     EVIVSRSELVQIGGGFRIPDILKASGAKLVEVGTTNITTLSDYLDAINEKTAMVLRVH
                     RSNFALRGFTEEVGVRDLKQHLPSDLLLVVDQGSGVFDRRIPSETTVGRHIASGADLV
                     SFSADKALGSVQAGCVVGKRELVAKLGKHPLFRTMRIGKTVSSLLEATLIDLLNGVDQ
                     PVGTLLFRSVAELRKTGRSILERIKSERLSLIEDTMTLGGGSTPDESLPSLSIRLESS
                     KPQKDAQGLRLRPVPIITTIHKGAVHINLGTVMYHETEELVSALGDLVEAP"
     gene            1788..3557
                     /gene="selB"
                     /locus_tag="PLF67_09285"
     CDS             1788..3557
                     /gene="selB"
                     /locus_tag="PLF67_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOE90242.1"
                     /translation="MYIIGTAGHVDHGKSALVKALTATDPDRLPEEKERALTIELGFA
                     SYTNKQGFVIGVIDVPGHERFIRNMVSGVWSLDGSLLCVAADDGWMQQSRDHALVLKA
                     MGTRLPMGVITKSDLVSEERLSVVKKQVANELRSIFGSEPIIHVISSKEGRGIEELKE
                     SIDSYLTKKKTTNFPPALALDRSFLIDGVGAVGTGSLRSMELKIGDEVTLLPSNLRAR
                     IRTLQSFGKNVERVKDGSRVAISLRGIEQAQLPKGAIITNDPTFYMCTQTVYVALQGT
                     GFKRISQLEIAAFTWHDQAKVRIIGKLESDTLLASVTTREIRPWYIGQKVVLIQSGAS
                     TIRASAQVITSSTLNKDQRTRLGALMNTDPRAIGELFTANRFLLWLNGYAQIKEATFT
                     GSLLGESYQKVGSWYLRSSLFESYTKNLMARLAKDRSVALAVVMQESPFPASLTQAIV
                     TSLVEKKQVVVKGTHLEPVRSEDPLSREEERLLQKITAAGLEGYPVKFLSREEKPVLG
                     HLRQRGAILIVESTFVYSKETFDRIVSLILKDKEEGTFFSIAEAKEHLPLSRKYILPL
                     LNKLEELGYVQRIGNERKVLKNA"
BASE COUNT          798 a         1099 c         1006 g          687 t
ORIGIN      
        1 tccgtcggca aggatggaaa caagctcatc aaatcccagg atctctatga cgcccttgag
       61 gcgaacccga aagccttcaa ggtaatcgac atccgaggcg agaaggactt caacgaaggt
      121 cacgtcatcg gctcgagcca caccgcctgg gcgcagttcg gatcgatcct tcccgagctc
      181 gacaagaagg ataagatcgt catcgtctgc ttcagcggcc agaccgccgg gcagaccgtc
      241 ggagtcctcc gcacgatggg cttcgatgca tactctctcc tcggcggcat caacaacggg
      301 tggaaacccg ccggtctccc gctggagaaa taatcgcatc cacaaaaggg gccgcttggc
      361 cccttttgga agcagatggg tcctggggat cccgcggtct tcaaaaccgt tggctgtagt
      421 aatcacagcg gcaggttcga ttcctgcctc ttccgtatgt atgaaaccct ccctcaagtg
      481 gaagccctcc ttcagcaccc ttcgatcggg aagtggttcg tccacgtcag ccgccccgtg
      541 gcggccgatg tgcttcggat cacgatcgaa gagtttcgaa agcggatcag ggttgaagaa
      601 aagggattcg atcttgatga aattgtaagc gcctgtgaaa aggcccttgc gacgatctcc
      661 tgccagcggc tgcaacgagt catcaacgcg accggcgtga tcatccacac caacctcgga
      721 cgggccccga tcagcaggga gaactgggaa cgcgtcggtc ttttgaacta ctcctacacc
      781 aacctggagt tcgacgtggc caagggcaaa cggggaaagc gctcttcgtt cacctctctt
      841 ttgttgacga aaatgagcgg tgccgaagac gccctcgtgg tgaacaacaa cgccgcggcg
      901 atctttctga ttctctccgc cttcgccaaa cgaaaggaag tgatcgtcag ccgatcggag
      961 ctggtgcaga tcggcggagg gttcagaatc cccgacatcc tcaaagcctc gggggcgaag
     1021 ctcgtggaag tgggtacaac aaatataaca accctctccg attaccttga tgcgatcaat
     1081 gaaaaaaccg cgatggtcct ccgggtccat cgctcgaact tcgccctccg ggggttcacc
     1141 gaggaagtcg gcgtccgtga cctgaaacag catcttccct ccgatctgct gctggtcgtc
     1201 gatcagggct ccggggtgtt cgatcgacgg atcccttccg agaccaccgt cggccggcac
     1261 atcgcctcgg gtgccgacct cgtctccttc tccgccgaca aggctctcgg ctcggtgcag
     1321 gccgggtgtg tcgtggggaa acgggaactg gtcgccaagc ttggaaagca tcccttgttc
     1381 aggacgatgc ggatcggaaa gaccgtctcc tccctcctgg aagccaccct gatcgacctg
     1441 ctcaacggag tcgaccagcc ggtgggcacg cttctgttca gaagcgtcgc tgaattacga
     1501 aagacgggaa gatcaattct ggaaagaatc aagagcgagc gcctctccct catcgaggac
     1561 acgatgaccc tcgggggagg atcgacccct gatgaaagcc ttccctccct ctcgatccgt
     1621 cttgaatcat ccaaacccca gaaagacgct cagggcctcc gcctccgacc ggtcccgatc
     1681 atcaccacga tccacaaggg ggctgttcac atcaaccttg gaacggtaat gtatcatgag
     1741 actgaagagc tggtttcagc gcttggcgac ctggtggagg ccccctagtg tacatcattg
     1801 gaaccgccgg ccatgtggac catggcaaat cggctctggt aaaggcattg accgcaaccg
     1861 accccgaccg gctccccgaa gagaaggaac gcgcccttac gatcgagctg gggttcgctt
     1921 cctatacgaa caagcagggc ttcgtgatcg gggtcatcga cgtgcccggc catgaacgct
     1981 tcatccgcaa catggtcagc ggggtctggt cgctggacgg ctcattgctg tgcgtcgccg
     2041 ccgatgacgg gtggatgcag caaagccgcg accacgccct cgttctgaag gcgatgggca
     2101 cccgcctgcc gatgggggtc atcaccaaaa gcgacctggt ttccgaagaa cgcctctcgg
     2161 tcgtgaagaa gcaggtcgca aacgagctcc gatcgatctt cggttccgaa ccgatcatcc
     2221 acgtgatcag cagcaaggaa ggacgaggga tcgaagagct gaaggaatcg atcgattcct
     2281 acctcacgaa aaagaagacc acgaactttc ctcctgccct cgccctcgac cgctccttcc
     2341 tgatcgacgg tgtcggggcg gtcggcaccg gatcgctccg ctcgatggag ctgaagatcg
     2401 gtgatgaagt gactcttctg ccatcgaacc tccgcgcccg gatccggacg ctccaatcgt
     2461 tcggaaagaa cgtcgagcgg gtgaaggacg gctcccgggt cgcaatctcc cttcggggga
     2521 tcgagcaggc ccagctgccc aaaggcgcga tcatcaccaa tgatcccacc ttctacatgt
     2581 gcacgcagac cgtgtatgtt gccctccagg gaaccggctt caaacggatc tcccagctgg
     2641 agatcgccgc cttcacctgg catgaccagg cgaaggtccg gatcatcggc aagcttgaaa
     2701 gcgacaccct tctggcctcc gtcaccactc gtgagatccg gccgtggtac atcggccaga
     2761 aggtggtgct cattcaaagc ggagcctcga cgatccgggc ttccgcccag gtcatcacct
     2821 cctcgaccct gaacaaggac cagcgcacca gactgggggc cctgatgaac accgatcccc
     2881 gcgccatcgg ggagctcttc accgccaacc ggttcctgct gtggctcaac ggatacgctc
     2941 agatcaagga ggcgaccttc accggctcgc tccttgggga atcataccaa aaggtcggtt
     3001 catggtatct tcgatcttcg ctgtttgaat cctacacaaa gaatctgatg gcccggcttg
     3061 cgaaagatcg gagcgtcgct cttgcggtgg tgatgcagga atccccgttt cccgcttcgt
     3121 tgacccaagc cattgtcacc tcccttgtcg agaaaaaaca ggtcgtcgtg aaaggcaccc
     3181 accttgaacc ggtcagaagc gaagacccct tgagcagaga agaagagcgc ctgcttcaga
     3241 agataaccgc cgccgggctt gaaggctatc cggtgaagtt cttaagccgt gaggaaaaac
     3301 cggttcttgg gcatctccgc cagcgggggg cgattctcat cgtcgaatcg acgttcgtct
     3361 actcgaagga gacattcgac aggatcgtct ccctgatcct caaagacaag gaggagggca
     3421 cgttcttctc gatcgccgag gcgaaggagc acctgcccct cagccggaag tacatcctcc
     3481 ccctgctcaa caagcttgaa gagctcggat acgtccagcg aatcgggaat gagcggaagg
     3541 tgctcaagaa cgcgtagtgc atgcatacta tgtctgataa accatgttcc
//
LOCUS       DAOSLI010000109         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_23329_length_3517_cov_13.997978, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000109 DAOSLI010000000
VERSION     DAOSLI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23329_length_3517_cov_13.997978"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..3400
                     /locus_tag="PLF67_09290"
     CDS             296..3400
                     /locus_tag="PLF67_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOE90243.1"
                     /translation="MRGWSQQGRQIAAVNRIRDFIHELKRSYVHSELAFSATRHTCDE
                     RLPLVDTFDLERLPIKEGERWGSAWQSSYFILSCDLPKEYEGKELAVRLNLGGEMLVY
                     SESGTPLYGLTSYSHYEKDYLKDTHRIGFHKGKVRLYVEVAANDLFGIERDEKKLDRS
                     GKHPEGVWNGIVAYARIGIFDDQRWKLFMASEFLFDLYQSIDKDSPRAVRVLGALFDA
                     ALIYPVRGTEAALSVLTEVLDKPAVPSALSTTAVGHAHIDTAWLWPIAETHRKVTRTF
                     ASQLRNLERYPSYLFGASTPQHYQWVKEEHPALYAQIRSAVASMRWEPLGAMWVEPDC
                     SLVSGESLVRQIVEGKQFFKEEFGIDVRNCWIPDVFGYPASLPQILRKADVQYFLTQK
                     ISWNAHTQFPYETFIWEGIDGSQVVTHFLPERTYNSGGRADGLIRAERGYLEKDRLDE
                     FLTAVGIGDGGGGPKDEHIERIILAKNLEGVPKASFGRVDRFFERLEAQKELLDLYRG
                     ELYLELHRGTLTTQARTKKNNRIFEEAIRVIESLFATQKRYPRETIKHVLRDGLMLQF
                     HDILPGSSIGKVYREADSLYARMFATLGELIVEYLESNKTDWNFISSSFFTRGRETKR
                     SKYLSIYRFNDSDDMQLVKIEGLDTDGRSYMLDGRYPLTRFGDSHWLYLPLARKGWNS
                     FVISEQHPVKLSKDSLILENEHITYEFAPNGQIIHAIDHATGRDRMHGASGNVLRLYA
                     DTPQKWEAWDIDFYYRQQEIETLQAVEHQYHVDNESFAALRFVYKTEHSTILQYAILP
                     KQGRQVYFKTHVDWNECRKLLRVEFPIDSMVEWASCDIPYGVLKRKTHIQDEKDFAQF
                     EFAARGFVDVGDGTTGAALLSDSKYGYSCRDGVLGMSLLRAPLDPDPFADLGEHSFVY
                     ALYPYADKANHVRALSNALNDPGVAVFSEEPIMLDAQPLSLEGDGVELSVLKQAETSA
                     ALVFRAVETGGQERFVTLHCASTLVFNEANLLEEAISDTEYRDGDQIAFMPFEIKTFV
                     QILR"
BASE COUNT          873 a          837 c          971 g          836 t
ORIGIN      
        1 cgatctggtg ataatgttca tgcttgctcc ttagaaatac agttcgtagt gtccaactct
       61 tatcattggc ttgggtgatg gaataagtca agtgaagatt gcaagcggat caaggaggat
      121 atagctatga gcataaaggt tttacatcag atttcaagcg gacatcgaac tgttatgtca
      181 tgcttttggc atatttgatt ttaatacgcg ttcgcgtatg atggggaaga acaagaaata
      241 aaaaacgacg atgttcttga tgaaagaata cgaatcggtt tgaaaggaag gtaccatgag
      301 aggatggtct caacaagggc ggcagattgc cgcggtcaac aggatcagag attttattca
      361 tgagttgaag cgaagctatg tccatagtga actggccttt tcggcgactc ggcatacgtg
      421 cgatgagcga ttgcccctgg tcgatacgtt cgatctcgag cggctgccga tcaaggaagg
      481 cgagaggtgg ggaagcgcct ggcaatcctc ctatttcatc ctttcctgcg atctgccaaa
      541 ggagtatgaa ggtaaagagc tggcggtccg gctcaatctg ggcggcgaga tgctcgtcta
      601 ttcggagagc ggcactccgc tctacgggct tacaagctac agccattacg agaaagacta
      661 cctcaaagat actcatcgga taggattcca caaagggaag gttcggctct acgtggaggt
      721 tgccgccaac gacctgttcg gcatcgagcg ggatgaaaag aagctcgatc gttcgggaaa
      781 gcatcccgaa ggggtttgga atggaatagt tgcatacgcc aggattggaa tctttgatga
      841 ccaacggtgg aagctcttca tggccagcga gttcctgttt gatctgtatc aatcgatcga
      901 caaggattct ccacgcgctg tccgggtgct tggcgccctg tttgatgcag cgctcatcta
      961 cccggttcgg ggaacggaag ccgctctatc cgttctgact gaagtccttg acaaacctgc
     1021 cgtgccttcc gcattgtcga ctacggcggt ggggcatgcc cacatcgata ccgcctggct
     1081 ctggccgatt gcggaaacgc accgaaaagt gactcggaca ttcgccagcc agcttcgaaa
     1141 tctggaacga tacccctcct atctcttcgg agcttcaact cctcagcact accagtgggt
     1201 caaagaagaa cacccggctc tctatgcgca aatacggagc gccgttgcct caatgcgatg
     1261 ggaaccgttg ggtgcgatgt gggtcgaacc ggattgctca ttggtatcgg gcgaatcatt
     1321 ggttcgccag attgtcgagg gaaagcagtt ctttaaggag gagttcggga tagatgtgag
     1381 gaactgctgg attccggatg tgttcggata tcccgcttca ttgccgcaga tcttgagaaa
     1441 agccgacgtt caatattttt tgactcaaaa aatatcatgg aacgcccata ctcaatttcc
     1501 ctatgaaaca tttatctggg agggaatcga cgggtcgcag gtggtaacgc actttctccc
     1561 ggagcgcacc tacaattcgg gcggaagagc cgacggtctt atccgagcgg agcgcggata
     1621 tctggaaaaa gatcgtcttg atgaattcct cactgcagta gggatcggag acggcggagg
     1681 cggaccgaaa gacgaacata tcgaacgcat tatcctggca aaaaacttgg aaggagttcc
     1741 gaaagcctcc tttggccggg ttgatcggtt ctttgagcgg ctggaggctc aaaaagagct
     1801 cttggatctc tatcgaggcg agctctatct ggagctgcat cggggaaccc tgacgaccca
     1861 agcgcgtacg aaaaagaaca accggatctt tgaagaagcg attcgggtca tcgagtccct
     1921 gttcgcaacc caaaagcgct atccccggga aacaatcaaa cacgtgttga gagacggctt
     1981 gatgctgcaa ttccatgata tccttccggg gtcaagcatc ggaaaggtgt acagggaggc
     2041 ggattcgctg tacgcaagga tgtttgcaac cctcggtgag ctcatcgtcg agtatcttga
     2101 gagcaacaag acggattgga atttcatatc gtcttcgttt tttactcgag gtcgggagac
     2161 caagcgatcg aagtatcttt cgatctatcg attcaacgac tccgacgaca tgcagctggt
     2221 caagatagag gggttggata cggatggaag atcatacatg ctcgatggaa gatatccgct
     2281 tacccggttc ggggattccc attggctgta tcttccgctc gctcggaagg ggtggaattc
     2341 atttgtcatc agcgaacaac acccggtcaa gctttccaag gactcactga tcctagaaaa
     2401 cgagcatatc acgtatgagt ttgctcccaa cggacagatc atacacgcga tcgatcatgc
     2461 aacgggcaga gaccggatgc atggcgcttc gggcaacgta ttgcgactct atgccgatac
     2521 tcctcagaaa tgggaggcct gggatattga tttttattat cgccaacagg agatcgagac
     2581 ccttcaggcg gtggaacatc aataccatgt ggacaatgaa tcatttgccg cgcttcggtt
     2641 cgtgtataag accgaacatt caacgattct ccagtatgcg atcctgccga aacaggggcg
     2701 ccaggtctat tttaaaaccc acgtcgactg gaatgaatgc cgcaaacttt tgcgagtcga
     2761 gttcccgatc gattccatgg tcgagtgggc ttcgtgcgat attccctatg gagttctgaa
     2821 gagaaagacc cacattcagg atgagaagga ttttgcccag ttcgaatttg ccgccagagg
     2881 gtttgttgat gtcggagacg gcacaacggg cgccgcgctg ctttccgatt caaaatacgg
     2941 gtattcatgc cgagacggag tgcttggaat gagcttgctc agggctccgc tcgatcccga
     3001 tccgtttgcg gatctcggag agcactcgtt cgtgtacgcg ttgtatccct acgctgacaa
     3061 ggccaatcat gtccgcgctc tttccaatgc actcaatgat cccggcgttg cagttttttc
     3121 cgaagaaccg atcatgctgg atgcacagcc gctttcgctg gaaggcgacg gggtggaact
     3181 gtcggtgctg aagcaagccg agacaagcgc tgcgttggta tttcgggcgg ttgaaacagg
     3241 cgggcaagag cgttttgtga cgcttcattg tgcttcaacc ctggtgttca atgaagccaa
     3301 cttgttggaa gaggccattt ccgatacaga atatcgagat ggagatcaga tcgcgttcat
     3361 gcccttcgaa atcaagacct ttgtgcagat actccgataa ggaggagcaa atagggcaat
     3421 caactccctt gacgttcctt tccattatcc gcataatggc aaggtaatca aaaccgcggg
     3481 gctttgcccc tcaggaagga gctctacagt gccttgt
//
LOCUS       DAOSLI010000110         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_23676_length_3472_cov_28.206029, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000110 DAOSLI010000000
VERSION     DAOSLI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23676_length_3472_cov_28.206029"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="PLF67_09295"
     CDS             complement(<1..1282)
                     /locus_tag="PLF67_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90244.1"
                     /translation="MIVLPVEEVRVEPSPIAAEEPAVFEPETAVVPPVVFTEELPPEP
                     ISVAIEEDVGAKPPAVIEKEPVSVLIVEEALIPEEEPSGYPFGIVPIIKSDDGSTSFD
                     LMIVHTNDVKGNIYTENGGLGLARLSTLMKMGRGYSDNLLFLNTGDVGSIAPEHEEIA
                     ALVVDEMGYDAYLPFEIQGTKNARSLAINALDEEGYFVVQPYQLYIYNGFVVGVVGVV
                     GPKDVPNVTFDSDLVLENARHAVDLAHTFVDYLIVLTDLGDGPFSSVDLAESIDGIDL
                     IIDGNGGETVLDVNNTLIVRGKEQLRSIGGLVLTIVDREVMLTQLLTFGAEDLLNPSA
                     SEILQAYGEMAEHYGTTLPEEIDEDPEIVALIGTLAEEPPVEEFVGPPPPPYVFDPLV
                     FTEEEYDFFYPTAPEPLPADDDFWADFFVVGEDSA"
     gene            complement(1450..2214)
                     /locus_tag="PLF67_09300"
     CDS             complement(1450..2214)
                     /locus_tag="PLF67_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HOE90245.1"
                     /translation="MNVRKNLVKRSVLLTLVLGALLVTSVYAYQFSPLEQTFQPSGAE
                     SSKTYTIVNDSNDSIAISISALTRDQDAQGNEINTPADAYFSIVPNKLVVPPQSSWVV
                     RVQYRGPQTVTTELAFRLKAEQIPYSQGRASTDKGMFNFLYIYTTSLYVQPSRVVESV
                     AVRSLAPATLEDGSPGLAVTVANLGTVHQLLIAATLEIRDNKGNTVLLEGQDALGSLD
                     GMNLLARKTVTKTIPWPNGLSSGSGVTYQATIKYTK"
     gene            complement(2301..2855)
                     /locus_tag="PLF67_09305"
     CDS             complement(2301..2855)
                     /locus_tag="PLF67_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOE90246.1"
                     /translation="MSTKARLARSLKELMAEQSLDRITVKEIVRRCGVNRQTFYYHFR
                     DMHDLLDWMFIHEGGEFLKKNPVYDYDDDGQSALKMICCYLQEHKSNVLSIYHSMGRE
                     LLCQYLCREIYKMLNKIVIRQGAAHKMSEFELHTILHFYKHAFVGSMLDWVEKGLPGT
                     PEDVVIRYRPLLKGMFDATMKKMA"
     gene            3009..3082
                     /locus_tag="PLF67_09310"
     tRNA            3009..3082
                     /locus_tag="PLF67_09310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3043..3045,aa:Pro,seq:ggg)
     gene            3226..>3472
                     /locus_tag="PLF67_09315"
     CDS             3226..>3472
                     /locus_tag="PLF67_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90247.1"
                     /translation="MDFMTAVQVLNGYLESIGIDLFWTVVVAVVAIGIIRRVTFGIAC
                     AAIAKSKGYRHGFWWGLLLPLLGLLVVALRPQKVVTVR"
BASE COUNT          827 a          905 c          856 g          884 t
ORIGIN      
        1 cggcgctatc ctcgccgacg acaaagaagt ccgcccaaaa atcatcatcg gcgggtagcg
       61 gttcaggcgc cgtcggatag aagaagtcat actcttcttc ggtgaatacc aacggatcga
      121 acacatatgg aggaggtggc ggaccgacga actcctcaac aggaggttct tcggccaagg
      181 ttccaatcaa ggcgacgatc tcaggatcct catcgatctc ttcgggaagc gttgttccat
      241 agtgttcggc catctctccg tacgcctgaa gaatctcgct cgccgacgga ttgaggagat
      301 cttcggctcc gaaggtcagc agctgggtca acatcacctc acgatcgacg atcgttaaga
      361 ccaatccccc tatggagcgc agctgctctt taccgcggac gatgagggta ttgttgacat
      421 ccagaaccgt ctcccctccg ttgccgtcga tgatcaagtc gattccatcg atcgactccg
      481 caagatccac cgaagaaaac ggtccgtcgc ccaagtcggt caggacgatc aggtaatcga
      541 cgaacgtatg ggcaagatcc acggcatgcc gggcgttttc aagaacgaga tccgaatcaa
      601 aggtgacgtt cgggacatct ttcggcccca caactccgac gacgccgacg acgaagccgt
      661 tgtagatata gagctgataa ggctgaacga cgaagtaccc ctcttcatcg agggcgttga
      721 tcgcaagcga ccgggcgttc ttcgttcctt ggatctcaaa cggcaagtat gcgtcgtagc
      781 ccatctcatc gactaccaac gccgcaatct cctcatgctc gggggcgatc gatccgacat
      841 caccggtgtt gaggaacaac aggttgtcgg agtaacccct tcccatcttc atcagcgtcg
      901 atagccgggc aagaccgaga ccgccgttct ccgtatagat gttgcccttc acatcgttgg
      961 tgtgcacaat catcaggtca aacgaggtgc tgccatcatc actcttgatg atcggaacga
     1021 ttccaaacgg atacccggag ggttcctctt caggaatcag cgcctcttcg acgatgagaa
     1081 cggaaacagg ttctttttca atgacggcag gaggctttgc gccaacatcc tcttcaatgg
     1141 caacactaat tggttcaggg ggaagttctt ccgtaaaaac aacgggaggc actaccgccg
     1201 tttccggctc aaataccgcc ggctcttcgg ctgcgatggg gcttggttcg accctaacct
     1261 cttccacggg taaaacaatc acctgttcct caatgtgagg aacaggtctt tcaggaggtc
     1321 tcgttaccat gtgatagagg aaaaacgcaa taatcaatac cccgatactc agaagcaatc
     1381 cgaggaaaac tgcgctttta ctagtttttc tgcccatcgc gagtcccttc tgctttggaa
     1441 ggtggtgcgc tatttcgtat acttaatggt tgcttgatac gttacacccg atccgctgga
     1501 taatccgttc ggccacggga tcgtctttgt tacagttttg cgagccagca ggttcattcc
     1561 atccaagctt ccaagagcat cttggccttc aagaagaaca gtgtttcctt tattgtcgcg
     1621 aatttccaat gttgcggcta tcaagagctg gtggaccgta ccaagattgg cgaccgtcac
     1681 ggccagaccg ggggatccgt cttcaagggt cgccggagca agggagcgaa ccgcgacact
     1741 ttcgacgact ctcgacggct gaacgtataa gcttgtcgtg tagatgtaga ggaagttgaa
     1801 catccccttg tcagtagagg cgcgcccctg tgaatacgga atctgttccg ctttcagtcg
     1861 gaaggcgagc tcggttgtta ccgtttgggg acctcgatat tgaacacgaa cgacccagct
     1921 ggactgaggc gggacaacaa gtttattggg aacaatcgaa aagtatgcat cagccggggt
     1981 gttgatctca ttgccttgag catcctggtc tcgagtcaat gcggaaatgc tgattgcaat
     2041 tgaatcgttt gaatcgttta cgattgtgta tgttttagag gactcagctc ctgaaggctg
     2101 gaatgtctgc tccaacgggg agaactggta cgcgtacacc gaagtaacga gtagcgctcc
     2161 caatacgagt gtaaggagga cactccgctt cactagattc tttctcacgt tcatatgaca
     2221 cactctcttt tcttttgccc aatgtttcgg ttagtgtacg ccatcaccac caaatcatca
     2281 agtatgcact cttactgact tcaagccatt ttcttcattg tagcatcaaa catccctttt
     2341 aacaggggtc gatagcggat aacgacgtcc tccggggttc ccggcagacc tttttcaacc
     2401 cagtcaagca tcgatccgac aaacgcatgc ttgtagaaat gcaggatcgt atgtagctca
     2461 aactcgctca ttttgtgggc agcaccctgg cgtataacaa ttttattcag cattttgtag
     2521 atttctctgc agagatactg acacaacaat tcccggccca tcgaatgata gatggaaaga
     2581 acgttgcttt tatgttcctg aaggtagcag cagatcattt tcaaggctga ttgaccgtca
     2641 tcatcataat catagaccgg attcttcttc aggaactcac ccccctcatg gataaacatc
     2701 caatccaata aatcatgcat gtccctaaag tgatagtaga aggtttgtcg gttgacgccg
     2761 catcgccgga cgatctcctt tacggtaatc cgatccagcg attgctcagc catcaactct
     2821 ttcaacgatc ttgccaatct tgctttggtg ctcaacagca atacctccgg atatcatgca
     2881 acaggttaca tcttttttag ggttactaca cctgtaaata gtacagcacc ttcagtgaga
     2941 tttctagaca aatattgttc aattgactaa agcaatcgtt acgtggtaag gttcgcatcg
     3001 ttcattgacg ggctgtagcg caggggttga gcgtacatgt ctgggggaca tggggtcgac
     3061 ggttcaaatc cgtccagccc gatttatcgt caagatcctg taaggtgtta ctccttgcag
     3121 gattttttgt gacccgggat cgttgcctca tccaccgttt tgctttggaa atccgctttc
     3181 gttctttcct gccgtatcct atactgaagg aaaaggagct gacctatgga ctttatgaca
     3241 gcggtgcagg tattgaacgg ctatttggaa tcgattggaa tagatctttt ttggaccgtg
     3301 gtggtagccg ttgtggctat tggtatcatc cgtcgagtga ctttcggcat cgcatgtgcg
     3361 gcgatcgcaa aatccaaggg atatcgccat ggcttctggt gggggcttct gcttcctctc
     3421 ctcggcctcc tcgtcgtcgc tctccgccct cagaaagtag ttaccgtccg aa
//
LOCUS       DAOSLI010000111         3445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_23917_length_3445_cov_15.709735, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000111 DAOSLI010000000
VERSION     DAOSLI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23917_length_3445_cov_15.709735"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..2969)
                     /locus_tag="PLF67_09320"
     CDS             complement(24..2969)
                     /locus_tag="PLF67_09320"
                     /EC_number="3.1.4.4"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF023335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004630 - phospholipase D activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE90248.1"
                     /translation="MSDFEKQLANGLYSGFLDRRRSSSEAMRPKLVLNNRLTQNRVAD
                     VLNTHLHTCSSFSFSVAFLSKGGLATLLQALLECKRRGVRGRMLTSDYLNFTDPRAVG
                     FIFRELGPEIEVRVYTAEAFHTKGYLFEHEERPTTLIIGSSNITQDALLRNHEWNLQV
                     AGTNQGQLIQDTKEEFERLWTNATTVSEAWLEHYTKVWDTFNTARVFVPRQVDSRSIQ
                     ETSVAKRGFELNSMQKDALARLKEIRSEGMSKALLVSATGTGKTILAASDVNQVKPER
                     FLFIIHREQIAHEAMRSFMQHLDEPVECALLTGSDRNLNAPYLFATIQTLTKDEVLYS
                     FDPDWFDYIVVDEAHHAPAASYRRVLEYFRPAFLLGMTATPVRPDGRDVHGIFGNTVA
                     AEITLSQALEEDLLAPFHYFGITGLSIDGTEGQKVEEFSNLEREEQIKHLSVILRRYS
                     LGLSRRRGLIFTSTNDDAAFVARELRGLGVRALQLSGASSQKEREEAFDRLEAEEEDP
                     HHLEYLVSVDIFNEGVDIPALNQIIMLRPTQSVIVFTQQLGRGLRKYPGKEFLTVVDF
                     VGNHQNNYMIPIALFGENSYDKDTLRRVVFGGAAIPGVSTVTFDRIAKERILDSINRA
                     SFSNRRMLTEAYQSVKNRLGNRIPSLVDFIRFDGINPELFLRYRRTWAQCKAVIDPAF
                     TLAMDDIHLKSLRFFSLYLPRGIRILEAGILSALLEGPKTEEALFARFKTNYGFVPTV
                     ASFASALRILNNEFVQEKYKESYGNISYVEEADGRYRLSREFSELLRNDQYRSELNEL
                     IQLGTLNHQLRFAGQCDDFGLVRGRKYTRSEVCRVLNWESDEGNTIYGYKVHGETNTC
                     PIFVTYHKHEGIDASIDYDDLFIDHQHFAWQSRTRVRLGSTEMQGIRDPEMKKLLFVK
                     KRDDEGAGHYYLGELGFLSNEQSSRLNNEGVELDVVNVRFRLFHPVEEKLYDYITDRE
                     QEEMVALDA"
     gene            complement(2966..3349)
                     /locus_tag="PLF67_09325"
     CDS             complement(2966..3349)
                     /locus_tag="PLF67_09325"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOE90249.1"
                     /translation="MRVVAAVIIKDGRIFAAQRNAFGPLAGKWEFPGGKIKEGESPVC
                     ALKREIREELGIEIEVHSPFLTVDHSYPTFDITLESYRCSLTEGTITLNEHLSCGWFS
                     LEELERLDWAAADLPIVSHLGRELG"
BASE COUNT          778 a          963 c          871 g          833 t
ORIGIN      
        1 ctgcttgaat cgtacagtcc cctctaggca tcgagggcga ccatctcttc ctgttcccgg
       61 tcggtgatat agtcgtagag cttctcctcc accgggtgaa aaaggcgaaa ccggacattc
      121 accacatcca gctcgacacc ctcattattt aggcgagaac tctgctcatt cgaaagaaag
      181 ccgagctcgc cgaggtagta gtgtcccgcc ccctcatcgt cacgcttttt gacgaacagg
      241 agttttttca tctccggatc tcggatcccc tgcatctctg tcgatccgag ccgaacccgg
      301 gtccgactct gccatgcaaa gtgttgatgg tcgatgaaga gatcgtcata atcgatcgaa
      361 gcatcgatac cttcatgttt gtggtacgtc acgaagatcg gacacgtatt cgtttcccca
      421 tgaactttgt acccatagat cgtgttgcct tcatcacttt cccaattaag cactcggcac
      481 acctcgctgc gggtatactt ccgtccacgc acaagaccga aatcatcgca ctgtccggca
      541 aaccgcaact gatggttcag cgtgcccagc tggataagtt cattcagttc gcttcgatac
      601 tggtcgttcc gcaaaagctc cgagaactcc cttgaaaggc gatagcgccc atccgcctcc
      661 tcgacgtagc tgatgtttcc ataggattcc ttgtacttct cctggacgaa ttcattgttc
      721 aggatccgaa gagctgatgc aaacgaagca acggtcggta caaacccata gttcgttttg
      781 aaccgggcaa acagggcttc ttccgttttc ggaccttcca gaagtgctga caagataccc
      841 gcttcaagga ttctgatacc gcgtgggaga taaaggctga aaaacctcag gctcttgaga
      901 tggatatcat ccatggcaag ggtgaaagcg ggatcgatca cagccttgca ctgagcccat
      961 gttctccggt agcggaggaa gagctccggg ttgatgccat cgaagcggat gaaatccacg
     1021 aggctcggta tccgattccc caagcggttt tttacgcttt gatacgcttc ggtcagcatc
     1081 ctcctgttgg agaacgacgc cctgttgatc gaatcgagga tccgttcctt cgcgatccgg
     1141 tcgaacgtga ccgtcgaaac gccggggatc gccgctcctc cgaagacaac acgcctcagc
     1201 gtatccttat catagctgtt ctccccgaag agggcgatgg gaatcatgta gttgttctgg
     1261 tggtttccca cgaaatcgac gacggtaagg aattcttttc cggggtattt tctgagtccc
     1321 cttccgagtt gctgagtaaa cacgatgacc gattgcgtcg gccggagcat gatgatctgg
     1381 ttgagcgcgg gaatatccac cccctcgttg aagatatcga cgctcaccag atattcaagg
     1441 tggtgcggat cttcctcttc cgcttcaaga cgatcaaacg cttcttcccg ctctttctgg
     1501 ctgctggctc cactgagttg aagcgcccga acgccaaggc cccgcaattc ccgggcgaca
     1561 aacgcggcat cgtcattcgt cgaggtgaag atgagtcctc ttcttcgact caagcccaag
     1621 gagtatcgcc tcaagatgac ggaaagatgt tttatctgct cttccctttc aagattgctg
     1681 aactcttcga ctttttgccc ttcagtgcca tcgatactga gaccggtgat gccgaagtag
     1741 tggaagggag ccagaaggtc ttcttcaagc gcctgagaaa gggtgatttc cgccgcaacc
     1801 gtattgccaa agattccatg gacatccctc ccgtccggtc ggacgggtgt ggcggtcatc
     1861 cccaggagaa atgctgggcg aaaatactca aggactctcc tgtagcttgc cgccggagcg
     1921 tgatgggcct catcgacgac gatgtagtcg aaccagtcgg ggtcgaacga gtacagcacc
     1981 tcatcctttg tgagcgtttg gatcgttgca aagagatagg gggcgttcag gttccgatcc
     2041 gagccggtca ggagggcaca ttccaccggc tcgtcgagat gctgcatgaa actccgcatc
     2101 gcttcatggg caatctgttc gcggtggatg atgaacagaa atcgctcagg ttttacctga
     2161 ttcacatcac ttgccgccag gattgttttt cccgtgcccg ttgccgaaac aaggagggct
     2221 ttgctcattc cttccgaccg aatttcttta agacgcgcaa gagcatcttt ctgcatcgaa
     2281 ttgagctcga aaccgcgttt ggcgacggaa gtctcctgaa tggaacggct gtcaacctgc
     2341 cgaggtacaa atactcgagc ggtattgaac gtatcccaca ccttggtgta gtgctcgagc
     2401 cacgcctccg agacagtggt tgcattggtc caaagacgct caaactcctc cttcgtatcc
     2461 tgaatcaatt ggccttgatt ggttccggct acctgaagat tccactcatg gtttctgagg
     2521 agggcatctt gagtgatatt ggatgatccg atgatcaggg tcgtcggcct ctcttcatgt
     2581 tcaaagaggt accctttggt gtggaacgct tcggcggtat acacccgtac ttcgatttcc
     2641 ggtccgagct cgcgaaaaat gaagccgacg gctcggggat cggtgaagtt cagatagtcg
     2701 ctggtgagca tccgtcccct caccccccgc cgtttgcatt cgagaagcgc ctgcaggaga
     2761 gttgcgagcc ctcctttgct taaaaatgcg acgctgaagg aaaaggagga acacgtatgc
     2821 aaatgggtgt tcagcacatc ggcaactcgg ttttgagtca gtcggttatt gagtacaagc
     2881 ttggggcgca tcgcttccga actggatctt cggcgatcga ggaagcccga atacaaaccg
     2941 ttggccagct gcttttcaaa gtcgctcatc ccagctcccg tccaagatga gacacgatcg
     3001 gaagatccgc tgccgcccaa tccagccgct ccaactcttc aagagaaaac catccgcaac
     3061 tcaggtgttc attcagggta attgtcccct ccgtaagcga gcaccggtag ctttccagag
     3121 taatatcaaa ggtcggatag gagtgatcga cggtaagaaa cggagaatga acctcgatct
     3181 cgattcccaa ctcttcccgt atttcccgct tcaatgcaca aacagggctt tccccttcct
     3241 tgatcttgcc accggggaac tcccactttc ctgcaagcgg accgaacgca ttccgctgag
     3301 cggcaaaaat ccgcccatcc ttgatgatga cagctgcaac gactctcata gatctgatct
     3361 tagcagcgcc gttttcattt gaccagggaa ttgagcagaa atcgtctctc tgctatggtt
     3421 ctccttatga acaaccccct cctgc
//
LOCUS       DAOSLI010000112         3341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_24869_length_3341_cov_25.853013, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000112 DAOSLI010000000
VERSION     DAOSLI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24869_length_3341_cov_25.853013"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1005)
                     /locus_tag="PLF67_09330"
     CDS             complement(52..1005)
                     /locus_tag="PLF67_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOE90250.1"
                     /translation="MRKIVVLDGYALNPGDLDWTPLEAYGELTVYDRTEPKDVVKRIG
                     DAEIIFTNKTILTGETIIQAPKLKYIGVLATGYNVLDMGAAREKGIVVTNIPAYSTDA
                     VAQFTFALLLEITNRVQRHSDAVIRDNRWTTCKDFCFWDYPLIELANKKMGIIGYGAI
                     GKAVGKVAKALNMEVLAYSPSLDPKHPDRASMETIYKESDIISLHLPLTEQSRGMINR
                     DTIAKMKDGVIILNTGRGPLVNEQDLADALNSGKVYAAAVDVVSVEPIKADNPLLKAK
                     NIIITPHIAWAPLQTRERLLGMAIRNVERFLAGKPINRVDL"
     gene            complement(1035..3158)
                     /locus_tag="PLF67_09335"
     CDS             complement(1035..3158)
                     /locus_tag="PLF67_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family protein"
                     /protein_id="HOE90251.1"
                     /translation="MDIYQDLLTRLKEVNQPLTEFFLDATYSEESFLTTLKERTEETL
                     KTVYPEGWAYLHGEKNFYRLSEPVLAHVRLYDYLVFDKAVFKDGTNEVTSRPVTLLRS
                     FLQKKSPTIHPDVAEEMVHFFALLSKDEPRTIPTRGQVQEWMERHPSGLDDEVIAWRK
                     KNKERIIDLLIRKIDERGSKEKRYTFKPGHSEKEKRWIVDGWWKEDRFHLYFALRSTK
                     ELDTFLGNTLDEETKRIMEAAEAKGIPIFVTPYFLSLIDTRPREEQEHPFADEPIRSY
                     LFYSRDLVDEFGEITAWEKEDAVEIGKPNAAGWVLPSHNIHRRYPNVAIFIPDTMGRA
                     CGGLCAYCQRMYDFQAGRFNFELEKLRPKKSWPARLKENMEYFRSDPYLWDILITGGD
                     AFMSSVKSLREILEAVLQMAKDKVEDNTKREEPYAVMKRVRLGTKLPVYLPQRVTGEL
                     ADTLAWFKRESAKIGIEQCVIQTHFSSAMEVTPDTEKAVGRILKAGWAVTNQEVFTVA
                     ASRRGHSAKLRKVLNDIGVLPYYNFTVKGFRENRALFATNARSVQELVEESSIGAIAP
                     RYHARIRSFIFNAKEMKEQIDSVRESDEIPFISPDRNTINLPGVGKSNTYRTIGLTDD
                     GRRILRFEFDHTRPHSKVIEEMHHVDIVESKSIARYLRQLEAMGEDPKEYESIWGYSA
                     GEMEERSPVFEGVIESKETPASPLL"
BASE COUNT          630 a          995 c          896 g          820 t
ORIGIN      
        1 tgtcggtctc aatgagggta aaaaggctga tgaaagatcg gaagcgtagc tttacaagtc
       61 caccctgttg atcggcttcc ccgccagaaa gcgttccaca ttcctgatcg ccatccccaa
      121 aaggcgctcc cgggtctgca gcggcgccca ggcgatgtgg ggggtgatga tgatgttctt
      181 cgccttcaga agggggttgt ccgccttgat cggctcgacg ctcaccacgt ccacggctgc
      241 ggcatagacc tttccggagt tcagcgcatc ggcaagatcc tgttcgttca cgagggggcc
      301 gcgcccggtg ttgaggatga tcaccccgtc cttcatcttt gcgatcgtat ccctgttaat
      361 catccccctg ctctgctcgg tcaggggcag atggaggctg atgatgtcgc tctctttgta
      421 gatcgtctcc atggaagccc ggtcagggtg tttcggatca agcgaaggcg aataggccag
      481 aacttccatg ttgagcgctt tggcgacctt tcccaccgcc ttgccgatcg ccccgtagcc
      541 gatgatcccc atcttcttgt tcgccagctc gatgagggga taatcccaga agcagaagtc
      601 cttgcaggtc gtccacctgt tgtcccgtat caccgcatcg ctgtgccgct gcacccggtt
      661 ggtgatctca agcaggaggg cgaaggtgaa ctgggccacc gcgtcggtcg agtaggcggg
      721 gatattcgta accacgatgc ctttttctct cgccgctccc atgtccaaga cattgtatcc
      781 cgtcgccaag acgccgatgt acttcagctt cggcgcttgg ataatcgtct ccccggtgag
      841 aatcgtcttg ttggtgaaga tgatctcggc atcgccgatc cgcttgacga catcctttgg
      901 ctccgtccgg tcatatacgg tcaattcacc gtaggcttca agcggagtcc aatccagatc
      961 cccggggttc agggcatatc catccagtac gacgatcttt ctcatggatg gaatcctacc
     1021 acgaagatcc gggattatag aagcggtgac gccggcgttt ccttggattc gatcacccct
     1081 tcgaagacgg gactccgctc ctccatctcg ccggcgctgt acccccagat cgactcatac
     1141 tccttcggat cctcccccat cgcctcaagc tgccggaggt accgggcgat cgacttggac
     1201 tcgacgatgt cgacgtggtg catctcctcg atcaccttgg agtgcgggcg ggtatggtcg
     1261 aactcaaagc ggaggattct ccgcccgtca tcggtaagcc cgatcgtccg gtaggtattg
     1321 gactttccca ctcccggaag attgatcgta ttgcggtcgg gggagatgaa ggggatctca
     1381 tcgctttctc tgacactgtc gatctgctcc ttcatctcct tagcattgaa gataaacgag
     1441 cggatcctgg cgtggtagcg cggcgcgatc gcaccgatgc tcgactcctc aaccagctcc
     1501 tgcaccgaac gggcgttcgt cgcaaacagc gcccggttct ccctgaaccc cttcaccgtg
     1561 aagttgtagt agggaagcac tccgatgtcg ttgagaacct tcctcagctt cgccgagtgt
     1621 ccccttcggc tcgccgccac ggtgaacacc tcctggttcg tcaccgccca gccggccttc
     1681 agaatccgac ccaccgcctt ttccgtatcg ggggtgacct ccatcgccga ggagaagtgg
     1741 gtctggatca cgcactgctc gatcccgatc ttcgcgctct cccgcttgaa ccaggcaagg
     1801 gtgtcggcaa gctccccggt gacccgctgg ggaagataga cgggcagctt cgttccgagg
     1861 cggacccgct tcatcacggc atagggctct tccctcttgg tattgtcttc aaccttgtcc
     1921 ttcgccatct ggagcaccgc ttccagaatc tccctcagcg acttcaccga gctcatgaac
     1981 gcatccccgc cggtgatcag gatgtcccag aggtacggat cgctgcggaa gtactccatg
     2041 ttttccttta gccgggccgg ccagctcttc ttcggccgga gcttctcaag ctcgaagttg
     2101 aagcgccccg cctgaaaatc gtacatccgc tggcagtagg cgcagagtcc cccgcacgcc
     2161 ctgcccatcg tgtcgggaat gaagatcgcg acgttcggat agcgccggtg gatgttgtgc
     2221 gagggcagaa cccaccccgc cgcattgggc ttcccgatct cgacggcatc ctccttctcc
     2281 cacgccgtga tctctccgaa ctcatcgacc agatcccggc tgtagaagag gtagctccgg
     2341 atcggctcat ccgcaaaggg atgttcctgt tcttccctcg gacgggtgtc gatcagggag
     2401 aggaagtatg gggttacgaa gatcggaatc cccttcgctt cggcagcctc catgatcctc
     2461 ttcgtctctt catccaacgt gttcccgaga aaggtgtcca gctcctttgt cgagcgcagc
     2521 gcgaagtaca ggtggaagcg gtcttccttc caccatccgt cgacgatcca tcgtttctcc
     2581 ttctcggagt gcccgggctt gaaggtgtag cgtttttctt tgcttccccg ctcatcgatc
     2641 tttcggatca gaaggtcgat gatccgctcc ttgtttttct tccgccatgc gatgacctcg
     2701 tcgtccagcc cgctggggtg gcgctccatc cactcctgga cctggccccg agtgggaatc
     2761 gttctcggct catctttgct caacagggcg aagaaatgaa ccatctcctc ggcaacgtcg
     2821 ggatggatcg tcggactctt cttctgcaga aaggatctaa ggagcgtgac cggtcgggat
     2881 gttacttcat tcgttccgtc tttgaagaca gccttgtcga agacgaggta atcgtacaag
     2941 cgaacgtggg caagaaccgg ttcgctgagc cggtagaagt tcttctcccc gtgaaggtat
     3001 gcccagccct cgggatagac tgttttcaag gtctcttccg tccgctcttt cagggtggtg
     3061 agaaatgact cctcgctgta tgttgcatcc agaaagaatt cggtcagcgg ttggttcact
     3121 tccttgagtc ttgttaaaag atcttgatag atatccatct taaaagggta gtattcgatg
     3181 ataattctgt caacccgaca gcctatttta ttatataata tatagttatg ttctctttgt
     3241 tttataaaaa ccgctggatg attctttcgt tttccctctg gagtcgggtt gacgaagcag
     3301 tgaaaaggtc gtatcaatat atgcgtatcg actgatatat t
//
LOCUS       DAOSLI010000113         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_25815_length_3249_cov_27.619912, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000113 DAOSLI010000000
VERSION     DAOSLI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25815_length_3249_cov_27.619912"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1728
                     /locus_tag="PLF67_09340"
     CDS             343..1728
                     /locus_tag="PLF67_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOE90252.1"
                     /translation="MKKFRIEHDSLGEKQVPVDAYYGVQTVRAMENFVISNTPISAFP
                     EYIKALGYIKKACALANMEFKILDKQIGEVIIKASEEMIAGKMDDQFPIDAIQGGAGT
                     SVNMNANEVIANRALELLGKERGVYDAVHPNNHVNLSQSTNDVCPSGIRIAIHHMLDD
                     LHDALKGLKKALADKGVEFSDVLKMGRTQLQDAVPMTLGMEFASWAVSVEEDIVRLKE
                     IQTLLTEINMGGTAIGTGINAPKGYAQVVTAHLATLTKLPLHPAKDLVQATQDSGTFV
                     EVSGFLRRIAVKVSKISNDLRLLSSGPRTGISEITLPPMQPGSSIMPGKVNPVIPEVM
                     NQVAFDVIGNDVAVAIAAEAGQLELNVMEPLIAYKIFTSIERLTRGLSTLTEKCVVGI
                     VANRDRSRSLVENSIGIITNLVPRLGYELCAQIAKKAQKTGGSVYEIVLEEGHLSKEE
                     LDQLLSPEAML"
     gene            complement(1783..2703)
                     /locus_tag="PLF67_09345"
     CDS             complement(1783..2703)
                     /locus_tag="PLF67_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90253.1"
                     /translation="MVRFVPDEGLWLKGNLHAHSTLSDGNLTPDRVIAAYEERGYDFF
                     ALTDHDYYARYAPREKMILIPGFELAGYMGSKRVHLNVFEKGPVSRFVQGQRFSIQDE
                     EETRAFIEEYQDEYLFMLNHPDWSLLEYRDIEAYDQFFALEVMNWGTEYYDGIGEGSY
                     FWETGLRYGRRWGAIATDDNHNGYTDEPGWPFGNTGNDSFGAWIMVKAKERTRQAIIA
                     ALEAGHYYASTGPEIHSFTVEDGWAEVHCSPVSRIIFSGEKRNLRRRLGENLTHFRAP
                     LRGNKEHVKVRIIDDKGKTAYSNPIYLKDV"
BASE COUNT          757 a          883 c          835 g          774 t
ORIGIN      
        1 gagaggtcgg ctgaatgaaa aagtcccgga aacaatcaaa aaaacggctc ctaaagcatt
       61 tcaagcatcc ctgccgaacc aaaacatcca acgccttctt gggtcgctca tcctatcgaa
      121 aggctgccaa tccggtagag attggttctc cgcagctggc cgcttctgaa cggttgaaac
      181 tgcaacgccc ttgacgggct cttcaaacgg aatcctcttt ttttatctat tccgaagaat
      241 accgatcttt gattgagttc tttttcaatg tcttttttca tcgttacggt gtacactgaa
      301 ggtagtgcta ttaccagttt ctatacacat ggaggagctt cgatgaaaaa gtttagaatt
      361 gaacacgatt ctcttggaga aaaacaggtg cccgtagatg catactatgg ggtgcaaacc
      421 gtccgggcga tggagaactt cgttatctcc aacaccccga tttctgcttt cccggagtat
      481 atcaaggctt tgggttatat caagaaagcc tgcgccttgg cgaatatgga gttcaagatc
      541 ctagacaaac agatcggcga ggtgatcatc aaggccagcg aggagatgat cgccggaaag
      601 atggatgacc agttccctat cgatgcgatt cagggcggag cgggaacttc ggtgaacatg
      661 aacgcaaacg aagtgatcgc caaccgcgcc ctcgagctgc tcggcaaaga acgaggcgtc
      721 tatgatgccg tgcaccccaa taaccacgtc aacctttcac aatcgaccaa cgatgtgtgt
      781 ccttcgggaa tccggatagc aatccaccac atgctcgacg acctgcacga tgctttgaaa
      841 ggattgaaaa aggcccttgc cgataaaggc gttgaattca gcgacgtgct gaagatgggt
      901 cgaacgcagc tgcaggatgc ggttccaatg accctcggga tggagttcgc ctcctgggcg
      961 gtatcagttg aggaggatat cgtccgattg aaagagattc aaaccctcct cacggaaatc
     1021 aacatgggag ggactgcgat cggcaccggc atcaacgcgc cgaaaggcta tgcccaagtg
     1081 gtcaccgccc atctggcaac gttgaccaag cttcccctgc atccggcgaa agaccttgtg
     1141 caggccactc aggattccgg aaccttcgtg gaagtgagcg gcttcctcag acggatcgca
     1201 gtgaaggttt ccaagatttc caacgacctt cggctccttt cctcgggacc gcggacggga
     1261 atcagcgaaa tcaccctgcc cccgatgcag ccgggatctt cgatcatgcc gggcaaggtg
     1321 aatccggtca tccccgaagt gatgaaccag gtcgcctttg atgtgatcgg caatgatgtc
     1381 gccgtcgcga tcgccgccga agcgggccag cttgaattga acgtgatgga gcccctcatc
     1441 gcctacaaga tcttcacctc gatcgaacgc cttacccgcg gtctatccac gctcactgaa
     1501 aagtgcgtcg tcggtatcgt cgccaaccgc gaccgctcgc gctcgctggt tgaaaactcc
     1561 atcggcatta taaccaatct ggttccgcgc ctcggctatg agctgtgcgc ccaaatcgcc
     1621 aagaaagccc agaagaccgg cggatcggtg tatgagatcg tgctggagga aggccatctc
     1681 agcaaggaag agctggatca gctcctctcc cccgaagcga tgctgtagat cgaaccatcc
     1741 cggtggaggc tcttcaccgg gatttctttt gctttccgct cgttacacat ccttcagata
     1801 gatcgggttg ctgtacgctg tcttgccctt gtcatcgatt attcgaacct tgacatgctc
     1861 tttgtttccc ctcagcggag cgcgaaagtg cgtgaggttt tccccaagtc tccgtctcaa
     1921 attgcgcttc tcaccactga agatgatcct gctcaccggt gaacaatgca cctccgccca
     1981 cccgtcttca acggtgaagg aatggatttc cggccccgtc gaggcgtaat aatgccccgc
     2041 ctcgagggcg gcgatgatcg cttggcgggt gcgctctttc gccttgacca tgatccaggc
     2101 gccgaaggag tcgttaccgg tattgccgaa cggccaaccc ggttcatccg tgtatccgtt
     2161 gtggttgtcg tcggttgcga tcgcacccca ccttcgtcca tagcgaagac ctgtttccca
     2221 aaaatacgac ccctcgccga ttccgtcgta atactcggtg ccccagttca tcacctcaag
     2281 ggcaaagaac tgatcgtacg cctcgatgtc gcgatactcc agaagcgacc aatcggggtg
     2341 attgagcatg aacagatact catcctgata ttcttcaatg aaagcgcggg tttcttcctc
     2401 gtcctgtatc gagaagcgtt ggccttggac gaatcgggag accggcccct tctcgaacac
     2461 gttgaggtgt acccgtttcg agcccatgta tccggcaagc tcgaagccgg gaatgaggat
     2521 catcttttca cgcggggcat atcgggcata gtaatcgtgg tcggtgagcg cgaagaaatc
     2581 atatccccgc tcttcatacg ccgcaatcac ccgatccggc gtcaaattgc cgtcactgag
     2641 agtggaatgg gcgtggagat tgcccttgag ccaaagacct tcgtccggaa caaaccgtac
     2701 catgcccatt tcgtccgcct gttcatctga tttcttgttg gcatcatacc atgtcgtcca
     2761 atcatcgaaa acttctcaca ggcggttatt tttggaatat gctctctatg ggcgtgtatc
     2821 ggtcggagac ggcgtgtttg ttttatcaag ataacggcct tatcggaaac agattttgaa
     2881 ggtaacgccg gtctcctgtt gtggaggccg gcataaagaa gtgttatttg aaatacatgg
     2941 tgattggcag tacgtgcagg agtgtctttg agctcctcga ggaacactaa aaagtcgtgc
     3001 aagtttgacg gacaggtaac cacggcatca atgttgatcc ggcttactcc gtcactgacc
     3061 gctttaaaca tggatattga gggtgaagtt gtagagctgg aggtatcggt cgattttttg
     3121 ggagatccat ctcttttgtc gtcatacttc gttacaaatc cttataatat atcgaactac
     3181 ttacgctatt tcacccgttc gtctttgccg ccagagccaa gagggccatc ccttggccat
     3241 actgcatcg
//
LOCUS       DAOSLI010000114         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_28099_length_3040_cov_25.404355, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000114 DAOSLI010000000
VERSION     DAOSLI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28099_length_3040_cov_25.404355"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="PLF67_09350"
     CDS             complement(<1..1580)
                     /locus_tag="PLF67_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE90254.1"
                     /translation="MERTIVSLLQSAAEKYQDRAYTNTRFDQGWVPSSFKQTNLDSDY
                     VAAFLLEDGVKSQMCVGILSEGKGQWVTFEHGALKARMVSVPLSIKLTAEEVAFRINH
                     SEAVMLAVSSNTLALVAAAFPSFDHPVKLVYLDAPDERLAKHVEEKKWKEGKDYVVWP
                     EILKKGEAILKKKPKLVSDSIAKIKEDDTINICYTSGTTGNPKGIMLTHLNYWANVQG
                     AVELFKIPDGTFETLIILPVDHSFAHTVGIYTSMVCGITLHFVDSRGSNAAIIRNFPK
                     NLVELNPTFLMTVPSITGSFMKKMTQGIAAKGPFIEGIFKRGLEAGIARNGDGFNKGS
                     KFKGWFHYHLANLLVFPKLRKVFGNKFLYCVGGGALLEARQQHFFAAIGSPVYQGYGL
                     TEAAPIICSNSPGRYKFGSSGMVLHNIECRIMVDDETEAKVGQRGEIVIRGDNVMKGY
                     FKNPEATAEALRDGWLWTGDLGYYDEDGFLVVTGRAKALLIGKDGEKYSPEEVEEVMV
                     NNVASINQLMVYNDHQAIT"
     gene            1773..2129
                     /locus_tag="PLF67_09355"
     CDS             1773..2129
                     /locus_tag="PLF67_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOE90255.1"
                     /translation="MPYTEPYDALDEKTRDISRAITSLKEEIEAVNWYNQRVYTTKDP
                     ELAAIMAHNRDEEIEHACMTIEWLRRNMDKWDEELRTYLFTDGSIIEAEESAMGRTGS
                     SSPKSGSSLSIGNLKK"
     gene            2144..2932
                     /locus_tag="PLF67_09360"
     CDS             2144..2932
                     /locus_tag="PLF67_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOE90256.1"
                     /translation="MDMFKRNLAPISNRAWSEIEERAKLVLLSRLSARKVVSVNGPHG
                     LDYTVLSEGRLSLVDDKSAVKVGIYNVLPLTEARVRFTLNKWELDNLEHGAKDIDFDS
                     LDKALDQLALFEENAVYHGYAPGTIKGLKESFAHKTLELTGDTKEFLAAIGEGVILLK
                     DSFVDGPYSLIVSNEVWVRLHKEAQGQSLKERVERIIGGEIVLASAIKGAILVPHDSA
                     DLELTIGQDFELGYESHDTKEVTLFATESFTFRVIEPKAIVVFK"
BASE COUNT          726 a          824 c          788 g          702 t
ORIGIN      
        1 gtgatcgcct ggtggtcgtt gtacaccatc agctggttga tcgaagcgac gttgttcacc
       61 atcacctctt cgacctcttc ggggctgtat ttttccccat ccttgccgat gagcaatgct
      121 ttcgcccggc cggttacgac cagaaagccg tcttcgtcgt agtagcccag atctccggtc
      181 caaagccaac cgtccctaag cgcctcggcg gtcgcttcgg ggttcttgaa gtatcccttc
      241 atcacattgt cgccgcgaat gacgatctcg ccccgctgcc ctaccttcgc ctcggtctcg
      301 tcatcgacca tgatccggca ctcgatgttg tgcaaaacca ttccgctgct tccgaacttg
      361 tagcgtcccg gggagttgga gcagatgatc ggggcggctt ccgtcagccc atagccctga
      421 tacaccggag aaccgatcgc cgcgaagaag tgttgttggc gcgcctccaa gagcgctccg
      481 ccgccgacgc agtacaggaa cttgtttccg aacacctttc ggagtttagg gaagactaaa
      541 agattggcca aatgataatg gaaccagcct ttgaatttgc tccctttatt gaagccgtcc
      601 ccgttgcggg cgattccggc ttcaagaccg cgtttgaaga tcccttcaat aaacggcccc
      661 ttggcggcga tcccctgggt cattttcttc atgaaggaac cggtgattga aggaaccgtc
      721 atcaggaaag tgggattcag ctcgaccagg tttttcggaa aatttcggat gatggcggca
      781 ttggaacccc gtgaatcgac gaagtgcagc gtgatgccgc aaaccatcga ggtatagatt
      841 cccaccgtat gggcgaaact gtgatcgacg ggcaggatga taagggtctc aaacgtccca
      901 tcgggaatct tgaacagttc gaccgctccc tggacgtttg cccaatagtt gaggtgggta
      961 agcatgattc ctttcggatt gcccgtcgtt ccgctggtat aacagatatt gatcgtatca
     1021 tcttccttga tcttcgcgat cgagtcgctg accagcttcg gcttcttttt cagaatggct
     1081 tcgccttttt tgagaatttc tggccagacg acatagtcct tcccttcctt ccacttcttt
     1141 tcttccacgt gcttcgcaag ccgctcgtcc ggggcgtcaa gatagaccaa tttcaccgga
     1201 tggtcgaatg aagggaatgc ggcggcgaca agcgccagcg tgttggaaga gactgcgagc
     1261 atcaccgctt cgctgtggtt gatccgaaac gccacttctt cagcggtaag cttgatcgaa
     1321 aggggaacac tgaccatccg ggctttcagc gccccatgct caaaggttac ccattgccct
     1381 tttccttcac tgagaattcc cacgcacatt tggcttttga cgccgtcctc gagaaggaac
     1441 gccgcaacgt aatcactgtc gagattcgtc tgcttgaagg aggagggaac ccagccttga
     1501 tcaaagcggg tgtttgtgta cgcacggtct tggtatttct ccgctgcgct ctgcaaaagg
     1561 gagacaattg ttcgttccat agtgctctct caacctgttt gtttttaata tatacattat
     1621 tataattatt gtcgaaaaaa acaagacgtc cttctcttta attcatttga tctatacacc
     1681 caccgagggt gaaggaaacg actccgcacc acttccattg aacctcttct aatagtacac
     1741 tgtatcagta acaaatgtaa caggaggaat gtatgccata tactgaaccg tatgacgcat
     1801 tggatgaaaa gactcgtgat atttcgcggg caatcaccag tttgaaagaa gaaattgaag
     1861 cagtcaactg gtataaccag cgggtctaca cgacaaagga tcccgaactt gccgccatca
     1921 tggcgcacaa ccgggacgaa gagattgaac atgcctgcat gacgatcgag tggcttcgaa
     1981 gaaatatgga caaatgggat gaggagctca ggacctatct ctttaccgac ggctcgatca
     2041 ttgaagcgga agaaagcgcc atgggaagaa cgggaagctc ttctccgaag agcggctcat
     2101 cgctttcaat cggaaacctg aagaaataaa aggaaggaat accatggata tgtttaaacg
     2161 aaacctcgct cccatatcca accgggcatg gagcgagatc gaggaacggg caaagctcgt
     2221 tttgctctcg cgcctgagcg cccgcaaggt cgtttccgtg aatgggcctc atgggctcga
     2281 ttatacggtg ctcagcgaag gcagactctc cctcgttgat gacaagagcg cagtaaaggt
     2341 gggaatctac aacgtgctgc cgctgaccga agcccgagtc cgcttcacct tgaacaagtg
     2401 ggaactggac aatctggagc acggggcgaa agacatcgac ttcgacagct tggataaagc
     2461 cctcgatcag ctcgctcttt ttgaggaaaa cgcggtgtat cacggctacg ctccgggtac
     2521 tatcaaaggg ctgaaggaat ctttcgccca caagaccctt gaacttacag gcgataccaa
     2581 ggagttcctc gcagcgatcg gagaaggcgt gattcttctg aaggacagct tcgttgacgg
     2641 cccctactcc ctcatcgttt caaacgaggt ctgggtgcgc ctccacaaag aagcgcaggg
     2701 ccagagcctc aaggaacggg tagagcgcat catcggcggc gagatcgtcc tcgcctccgc
     2761 gatcaaagga gcgatcctcg ttccccacga cagcgcggac ctggagctca cgatcggtca
     2821 ggactttgaa ctcggctacg agagccatga caccaaagag gtcaccctct tcgccaccga
     2881 gtcgttcacg ttccgcgtca tagaacccaa ggcaatcgtc gtattcaagt aatcgatttg
     2941 cgggatagac ggagaaagac cccgctcact gagtggggcc tttcttcgag cgccagaagg
     3001 gaatcgaacc ctcgtctcat gcttgggaag cacgagcact
//
LOCUS       DAOSLI010000115         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_28980_length_2967_cov_29.114698, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000115 DAOSLI010000000
VERSION     DAOSLI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28980_length_2967_cov_29.114698"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..929)
                     /locus_tag="PLF67_09365"
     CDS             complement(102..929)
                     /locus_tag="PLF67_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE90257.1"
                     /translation="MRITTLVENVSTDPLLGSEHGLSLWVETEAKKILFDSGATTLFE
                     ENARVLGVDLSKADFAVLSHGHWDHSGGMNRFLALNGHAPIYLKETALGSFYSKRDEE
                     TLVYAGLDEELKQSDRFIFSDEYTDLGDGAFLFGRIEGTEFVPEGNRTLYVERDEVMT
                     VDPFDHEHYLGIEENGEKVLFSGCSHLGIVNIVEQYRLLKGHYPTRVIGGFHLSKIDP
                     HNSDDFHILKEIAARLLESGAIFYTGHCTGEASYQILKEYMQEHLEYLPTGRVLEFP"
     gene            complement(926..1780)
                     /locus_tag="PLF67_09370"
     CDS             complement(926..1780)
                     /locus_tag="PLF67_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE90258.1"
                     /translation="MKQLLLLSGKGGTGKTTVATGLIQLSQAKAYADCDVDAPNLHLV
                     LGTFDNHATKPYYDLPKAVIDPNRCTECGLCYQVCRFDAIAENPYRVIPHLCEGCTFC
                     THVCPAGAIALEETVAGTIELHTRGDETFSTATLKMGSGTTGLLVTEVKKQIKDKAEF
                     AILDGSPGIGCPVIASLTGVDLVLMVAEPSLSGISDLKRVIQNARQLQVPICVIVNKS
                     DVNEELTERIRHYCAKEALPFLGTIPYDREAVAAINSGQSLIERGGPASEAIQEIYHK
                     VMKELEAL"
     gene            complement(1752..2597)
                     /locus_tag="PLF67_09375"
     CDS             complement(1752..2597)
                     /locus_tag="PLF67_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE90259.1"
                     /translation="MKIAVLSGKGGTGKTLVSVNLAAVAEKGGYFDCDVEEPNGHLFL
                     KPSDIEEKEVTSLKPVVDMDKCDGCRVCVDACVYKALAYIPGELMIFDEICHACGLCT
                     HLCPRQALTETKRVIGTIRRGRRGSLSVHTGTMHIGESTGVPIIKELLKKLDDDLSII
                     DSPPGSGCLVLETIAHADYCILVAEPTIFGARNLALVHELTQVMNKPAGVLLNKVEDG
                     INPSELYAKEKTLKILGSLPYDSYVALENSNAELLVERSEKYRTFFLELLKEVVDEAT
                     LAAQR"
     gene            complement(2601..>2967)
                     /locus_tag="PLF67_09380"
     CDS             complement(2601..>2967)
                     /locus_tag="PLF67_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE90260.1"
                     /translation="VMIIAIPAEEKSLDSVISINFGRSPYYCIYDTEKEQSTFVENKA
                     VERSGGAGVEAAQDLVDRKIDALVTFRLGDHASKVLTAAKIKVLKAVNLSIADNIAKV
                     LDHSLEELNEVHPGGINKG"
BASE COUNT          564 a          844 c          848 g          711 t
ORIGIN      
        1 ccgtcctcga cggtgaatac ttcaaaggtc tcacagtgtc cgaaatggcc acagacgtgc
       61 ttgttctcgc ttgctactgc aatctttttc attgttgact cctatggaaa ctcgagcacc
      121 cgtccggtcg gaaggtactc gaggtgttcc tgcatgtatt ccttcaatat ctgatagctc
      181 gcctccccgg tgcaatggcc ggtatagaag atcgctccgc tttcaagaag ccgggcggcg
      241 atctccttga ggatatgaaa gtcatcgctg ttgtgggggt cgattttgga gaggtggaac
      301 cctccgatga cccgggtggg gtagtgtccc ttcagaagcc tgtactgctc gacgatgttc
      361 acgattccca gatggctgca tccgctgaaa aggacttttt ctccattttc ctcgattccg
      421 aggtagtgtt catggtcgaa gggatcgaca gtcatcacct cgtccctctc gacgtagagc
      481 gtcctgttcc cctcgggaac gaactccgtt ccttcgatcc tgccgaagag aaacgccccg
      541 tcgccaagat cggtgtactc atcgctgaag atgaagcggt cgctttgctt gagctcctca
      601 tccagaccgg cgtacaccaa ggtttcttca tcccgtttgc tgtaaaagga accgagggcg
      661 gtctctttca ggtagatcgg cgcatgccca ttgagggcga ggaagcggtt catccctccg
      721 ctgtggtccc agtgtccgtg ggaaagaacc gcaaagtcgg ctttggaaag atcgacgccc
      781 aagacccggg cgttctcttc aaaaagggtg gtggccccgc tgtcgaaaag gatcttcttc
      841 gcctccgtct cgacccacaa cgaaaggccg tgttccgaac ccaagagggg gtcggtggag
      901 acgttctcca cgagcgtcgt gattctcata gtgcttccag ctccttcatc accttgtgat
      961 agatctcttg aatcgcctcg cttgccggac cgccgcgttc gatgagcgac tgtccgctgt
     1021 tgatcgcagc gaccgcttcc ctgtcgtagg ggatcgtccc caggaaggga agagcctcct
     1081 tggcgcaata gtgcctgatt cgctcggtaa gctcctcgtt cacatcgctt ttgttcacga
     1141 tgacgcagat cggcacctga agctgccggg cgttttgaat cacccgcttc agatcgctga
     1201 ttcccgaaag cgagggttcg gcgaccatca gaaccagatc gaccccggtc agggaggcga
     1261 tgacgggaca cccgatgccg gggctgccgt cgaggatcgc gaactccgcc ttgtctttga
     1321 tctgcttttt cacctcggtg accagaagcc cggtggttcc gctacccatc ttcaatgtcg
     1381 cggtggagaa cgtctcgtcg ccccttgtat gaagctcgat cgttccggcg acggtctctt
     1441 cgagggcgat cgccccggcg gggcacacgt gggtgcagaa ggtgcatcct tcacagagat
     1501 ggggaatcac ccgatagggg ttctcggcga tcgcatcgaa gcggcagact tgataacaca
     1561 agccgcactc ggtgcaccga ttggggtcga tgaccgcttt gggcaggtcg taatagggtt
     1621 tggttgcgtg gttgtcaaac gttcccaata ccagatggag gttcggggca tccacgtcgc
     1681 agtcggcgta cgcctttgcc tgactgagtt ggatcaggcc ggtcgcgacg gtggtctttc
     1741 cggttccgcc tttaccgctg agcagcaaga gttgcttcat cgacaacctc cttcaggagt
     1801 tcaaggaaga acgtccgata cttctcgctt cgctcgacga ggagctcggc gttggagttc
     1861 tccagcgcca catatgaatc atagggcagg gacccgagaa tcttcaaggt tttttccttg
     1921 gcgtagagct ccgacggatt gattccgtct tcgactttgt tgagcaatac ccccgccggc
     1981 ttgttcatca cctgagtcag ctcatggacc agggcgaggt tccgggcgcc gaagatcgtc
     2041 ggctcggcga ccagaatgca gtagtcggcg tgggcgatcg tctcaaggac cagacacccc
     2101 gagcccgggg ggctgtcgat gatggaaaga tcgtcatcga gttttttcag gagttccttg
     2161 atgatcggga ccccggtgct ttcgccgatg tgcatcgttc ccgtatggac ggaaagggag
     2221 ccgcgcctgc cccgccggat cgtcccgatc acccgtttcg tctccgtgag cgcctgccgg
     2281 ggacacaggt gggtgcagag cccgcaggca tggcagatct catcgaaaat catcagttcg
     2341 ccggggatgt aggcgagggc tttatacacg cacgcatcga cgcagaccct gcatccgtcg
     2401 catttgtcca tatccacgac gggcttgagg gaggtgacct ccttttcttc aatgtcggac
     2461 ggcttcagga agaggtgtcc gttgggctct tcgacatcgc agtcgaagta tcctcctttc
     2521 tccgctacag cggccaggtt caccgaaacg agggttttgc ccgttccgcc tttgccgctg
     2581 agaaccgcta tcttcatata tcatcctttg ttgatcccgc cggggtggac ttcgttcagc
     2641 tcctcaaggg aatgatcgag gactttggcg atattgtcgg cgatggaaag gttcaccgct
     2701 ttcaacacct tgatcttcgc ggcggtgagc acctttgatg catggtcgcc gaggcggaag
     2761 gtgacaaggg catcgatctt ccgatccacc agatcctggg cggcttcgac tcccgctcca
     2821 ccggagcgtt cgacggcttt gttctcgacg aacgtcgact gttctttttc cgtatcatag
     2881 atgcaataat agggactgcg tccgaagttg atgctgatga cgctgtctaa acttttttct
     2941 tctgccggaa ttgcgataat cattacc
//
LOCUS       DAOSLI010000116         2946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_29257_length_2946_cov_26.500865, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000116 DAOSLI010000000
VERSION     DAOSLI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29257_length_2946_cov_26.500865"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..2830
                     /gene="greA"
                     /locus_tag="PLF67_09385"
     CDS             110..2830
                     /gene="greA"
                     /locus_tag="PLF67_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOE90261.1"
                     /translation="MGIQEIEQVLKEEQWTRASASAFTVANFETFDRLIEDLDDEQKI
                     EARDMCERHIREREKNSVVAHYINGMLAIQRKGSENFLPLLNLIELFSEHKRWNIVEY
                     LAQKILNKSENRHALRLLAQSYEATGKQEKKIELWERLVNVDYEEVDILKQLGTYALE
                     AGKTEKAVSYFKKTINRLLKRKDFTPIRHIHEIVITSDPHQFDYTMNLAEQISVTSHS
                     SAVAILRDATEAYKEDVDQMIEIQKQILMLDREDAIARDQLIKSYRKKYKDHSRLESC
                     LEASALVGMIARDVLLSIEDFETNIAFDKGTFVFQNSTGRIGRIRSIDETDVIVDFAG
                     QATTQGSRLSTNMAFKSLQALPKSHIWVLKSALPKQRLYDKVKSDIPWTLNILMSSNG
                     GQSSLREMKAELVPSILEAKEWTSWMNQAKKELMANPLFDLSPTDSDVYLLRSTPISY
                     EEKQLSIFRSEREFYDKIRRLREFISSKGDVESDYFLEMVGFFSEFFFEENGVSKPIV
                     VTNDVIMSSYLLLEELVKKQGMHFVTLPEAFGFTDLFERANNIYEVFAPIKDSELKKS
                     FIDHVVDEIPSWPKVLMEIFPGYLTSYIPEIFRQNKKNADLLKLYKDAVENFKDKGAT
                     LIYLLKNGDKKVWDKSGYTKEQLLYIQLQLLDTVNRYIDAKRDAQENRKNSKALMSML
                     FDDKQIFTCIEEGNEENAQRIYSLVANVFDLPGGKKIEVKHSISVKFPGFKFFDEAPL
                     SQREAFVPTGLFCTAASLEAKKKELDYIQHVELPEIAKEIGAAREMGDLRENSEYKYG
                     KEKQTLIQNNLRRLSEEIDRASVVTPDMVDESKVGFGTKVTLMDNLKGEEVTYTIMGQ
                     WEANPNENIINISAPFGRELVNHEVDERFTFTINEQSYDFTVRKIEKIDF"
BASE COUNT          859 a          696 c          733 g          658 t
ORIGIN      
        1 tatactttgc aagatagata gaaagaagtt ttggactggg cagacaaaga cgaatgtctg
       61 cacggttttg ttttgtctat taagacgata tattttcgga gacaagatca tgggaatcca
      121 agaaattgaa caagtactca aggaagaaca atggaccaga gcctcagctt ccgccttcac
      181 ggtggccaat tttgaaacat ttgaccggtt gattgaagat ttggatgatg agcagaagat
      241 cgaggctcgg gatatgtgcg agcggcatat tcgggaacgg gaaaagaatt cagtagttgc
      301 ccactatatc aatggaatgc tcgccatcca acggaagggg tcggaaaact tccttcccct
      361 tttgaatctc attgaactct tcagcgaaca caagcgctgg aacatcgtgg aatacctcgc
      421 ccagaaaatt ctaaacaaaa gcgaaaaccg ccacgccttg agactgctcg cccaatcata
      481 tgaagcgacc ggcaaacagg agaagaagat cgagctgtgg gaacgactgg tcaatgtcga
      541 ttatgaagag gttgatattc tcaagcagct tggaacgtac gctcttgaag ccgggaagac
      601 ggaaaaagct gtcagttact tcaagaaaac gatcaatcgg ttgctgaaac gaaaagactt
      661 taccccgatt agacatattc atgagatcgt catcaccagt gacccgcacc aattcgatta
      721 cacgatgaac cttgctgaac aaatctccgt tacgagccac tcctccgccg tggcgatctt
      781 aagggatgct accgaagcgt ataaagagga tgttgatcag atgatcgaaa tccaaaagca
      841 aatccttatg ttggaccggg aggatgcgat cgcccgcgat caattgatca agagctatcg
      901 aaaaaaatat aaggaccact cccgcttgga aagctgtctt gaagcctccg ccctcgtagg
      961 catgatcgcc cgggacgtgc tcttgtcgat tgaggatttt gaaacaaaca tcgcattcga
     1021 caagggaacg ttcgtattcc agaactccac cggacggatc ggaaggattc gatctatcga
     1081 tgaaacggat gtgatcgtcg acttcgccgg gcaggcgacc actcagggtt cccgtttgtc
     1141 gaccaacatg gcattcaaga gcttgcaggc cctgcccaag agccatatct gggttctgaa
     1201 aagcgccctt ccaaagcagc ggctctacga caaagtcaag agtgatattc cttggacgtt
     1261 gaacattctt atgtcaagca atggagggca atcgagtctt cgggagatga aggcggaatt
     1321 ggtgccttcg atcctggagg cgaaagagtg gacaagctgg atgaatcagg caaagaaaga
     1381 gttgatggcc aatcccctct ttgacctctc ccccaccgac agcgatgtgt atctgcttcg
     1441 atccaccccg atcagctacg aggagaaaca gctttcgatc ttccgaagcg aacgggaatt
     1501 ctatgataag attcgtcgtt taagagagtt catttccagc aagggcgatg tggaaagcga
     1561 ttacttcctt gaaatggtcg ggttcttcag cgagttcttt tttgaagaaa acggagtctc
     1621 aaaaccgatc gttgtcacca atgatgtgat catgtcctcc tatcttctcc ttgaagagtt
     1681 ggtcaagaag caagggatgc attttgtcac ccttcccgag gcgttcggct ttaccgatct
     1741 ctttgaacgg gcgaataaca tctatgaagt ctttgctccg atcaaggact cggagctgaa
     1801 gaagtccttc atcgatcacg tcgttgatga aatcccctcg tggccaaagg tcttaatgga
     1861 gatctttccc ggctatctta caagctatat tcctgaaatc ttccgccaga acaagaagaa
     1921 cgccgatttg ctcaagctgt acaaggatgc ggtggagaat ttcaaggata agggtgcaac
     1981 cctgatctac cttttgaaga atggcgacaa aaaagtgtgg gacaagagcg ggtataccaa
     2041 ggagcagttg ttgtatatcc aactccagct gcttgatacg gtgaaccgct acatcgatgc
     2101 caaacgcgat gcgcaagaga atcgaaagaa ctcaaaagcg ctgatgagca tgcttttcga
     2161 tgacaagcag atcttcacct gcattgaaga aggtaatgaa gaaaacgccc aacgaatcta
     2221 ctcgctggtg gcgaacgtct tcgatcttcc cggtggaaag aaaatcgagg tcaagcattc
     2281 aatcagtgtt aaattccccg gattcaagtt ctttgatgaa gccccgctct cccagcggga
     2341 agcgttcgtt cccaccggac tgttctgcac cgcagcttct ttggaggcca aaaagaagga
     2401 gttggattat atccagcacg tcgagctgcc ggagatcgcc aaggaaatcg gggccgcccg
     2461 cgagatgggc gacctgagag agaacagcga gtacaagtac ggaaaggaaa aacagaccct
     2521 gatccagaac aatcttcgcc ggctctcgga ggagatcgac cgggcctcgg tcgtcacccc
     2581 ggacatggtc gatgaatcaa aggtcggatt cggcaccaag gtcaccttga tggacaatct
     2641 gaaaggcgaa gaggtcacct acacgatcat gggccaatgg gaggcgaacc ccaacgagaa
     2701 catcatcaac atctccgccc ccttcggccg ggaactggtg aaccacgagg tcgatgaacg
     2761 cttcacgttc acgatcaacg agcaatcgta cgactttacg gtcagaaaga tcgaaaagat
     2821 cgatttttaa ggaaccttca aacaatcatt caatcgcccc caaacggggg cgatgactat
     2881 attgttaaat acatttaaca aaaacacatc aaaattgtta gatggacttt acaaaaaggc
     2941 catatg
//
LOCUS       DAOSLI010000117         2911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_29701_length_2911_cov_22.208683, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000117 DAOSLI010000000
VERSION     DAOSLI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29701_length_2911_cov_22.208683"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..775
                     /locus_tag="PLF67_09390"
     CDS             113..775
                     /locus_tag="PLF67_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90262.1"
                     /translation="MTRRAKLITTTLLLFLVLLGGCTSTKQVKPLGGEQLHRQLIATL
                     IEAAYGMDTTTLMAKTVEELLPANALPMNRIAGIPLFEEQLSRFKDEVNSAFLSTAWE
                     IAPLLEPIIQTIPWSDDLALVASLRSGAATLQRESRDELLSVITPILSDALKPAASRW
                     TALIDWYHLYRESVKLLNIEVLPVLDEDIKEHLTLVYLERYCAALGDEEHRLRSQTTE
                     VR"
     gene            775..2907
                     /locus_tag="PLF67_09395"
     CDS             775..2907
                     /locus_tag="PLF67_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE90263.1"
                     /translation="MELFSATKSGSEPLAYRMRPRTLEEYVGQEAIIGEGRLLRRAIQ
                     ADQLSSVIFYGPPGTGKTTLARVIANSTKRHFATLNAVLSGVKELRFEIDAARERLEL
                     YQRGTILFVDEVHRWNKSQQDALLPWVENGTVILIGATTENPFFEVNAALVSRSRIFQ
                     LKPLTGDDLMKIAHMTLSDKERGYGNYEITFEDGALEHLVDVSDGDARTLLNALELAV
                     ETTGEPDEPKYISLAAAEDSIQQKAVLYDKEGDYHFDVISAFIKSIRGSDPDAALYWL
                     ARMVHAGEEPRFIFRRMLISAAEDIGLADPNALVVVEAAAAAFERIGLPEGRFHLAQA
                     ALYLATAKKSNSTMGFFDALKKVTEEAQREVPNHLRDNSRDAKGFGHGEGYLYPHAYS
                     GHWVAQQYLPAALQGKIFYNPGDQGYEATIASEVQGRREEQLESIQSDVFVEHLSYSP
                     PSSVEAIWRERSAGERSTILRAIRKELFREHTVRRSDRVFIANAGSGFLLWEALRLTP
                     EGLCVAQVSTKQHEEVINHYAKTLEMLERPHVHIGDPASVLRSLHERLLFEHIYGRNI
                     LSRLHAEKEILELFFEKLVKGGTLNIAQSIPSRSSRLSEFCDHPLLKEAEKVIYADET
                     NPLINWDEKDLEEAAKSYGFQGKTEVLSFTENRVFTSDQLEGYLKNSYLPALKNEDPS
                     FVVSVIKALEKKSLAYRQSIAILRLAKD"
BASE COUNT          687 a          854 c          788 g          582 t
ORIGIN      
        1 agaaagagcc tcctcgacag gcgttctcca aaagatgctt tgcgcttatt ggaaataaag
       61 catatagttt tgaatgaaga tgatcttcac tgttataagg agctgaactg agatgactcg
      121 ccgggccaag ctgattacga ccactcttct tctcttcctc gtcctgcttg gcggatgcac
      181 gtcaacgaaa caggtgaagc cccttggggg cgagcagctc catcgacagc tcatcgccac
      241 cttgatcgaa gcggcgtatg gaatggatac gacgaccctc atggcgaaaa ccgttgagga
      301 gctccttccg gcaaacgccc ttcccatgaa ccggatcgcc ggcatccccc tgtttgaaga
      361 acagctttcc cgcttcaaag acgaagtgaa cagcgccttt ctttccaccg cttgggagat
      421 cgctcccctc ttggagccga ttatccaaac aataccctgg agcgatgacc tcgcccttgt
      481 cgcatccctc cgctcaggag ccgccaccct tcaacgggag agtcgggatg agctcctatc
      541 ggtcatcacc ccgattctca gcgatgccct gaagccggcg gcaagccgat ggacagccct
      601 catcgattgg tatcacctct atcgggaaag cgtcaaactc ttgaacattg aagttcttcc
      661 cgtgttggat gaggatatca aggagcattt gacgcttgta tatctggagc gctattgcgc
      721 cgccctcggc gatgaagagc atcgcctccg cagccaaacg accgaggtac ggtgatggaa
      781 ctgttttccg cgaccaaatc cggatccgag ccgctggcat accggatgag accccgaact
      841 ttggaggagt atgtgggtca ggaagcgatc atcggggaag gacggctcct cagacgggcg
      901 atccaggcgg accagctctc ctcggtcatc ttctacggac ctccggggac gggaaaaacc
      961 accctcgccc gggtgatcgc caactcgacc aagcggcact tcgccacctt gaacgccgtc
     1021 ctcagcggag tgaaggagct ccgctttgag atcgatgcgg cacgcgaacg tcttgaactg
     1081 tatcaacgag ggacgatcct cttcgtcgat gaggtgcatc gctggaacaa gagtcagcag
     1141 gacgccctcc ttccctgggt ggaaaacggg acggtcatcc tgatcggcgc cacgacggaa
     1201 aaccccttct ttgaagtcaa tgccgccttg gtgtcccgct caagaatctt tcaactcaag
     1261 ccgctgaccg gggacgatct gatgaagatc gcccatatga cgttgagtga caaggaacgc
     1321 ggctacggca actatgagat aacctttgaa gatggagcgt tggagcacct tgttgatgtc
     1381 agcgacggtg atgcccggac ccttttgaac gctcttgagc ttgctgttga gacgacggga
     1441 gaaccggatg agcccaaata catctccctc gcagcagccg aggattcaat ccaacagaaa
     1501 gcggttctct acgacaagga aggggattat cactttgacg taatcagcgc gttcatcaaa
     1561 tcaatcaggg gcagcgatcc cgatgccgcc ctctactggc ttgcccggat ggtccacgcc
     1621 ggcgaggagc cgcggttcat ctttcgaaga atgttgatca gcgccgccga agacattggt
     1681 cttgccgacc ccaacgcact ggtggtggtc gaggccgccg ccgcggcgtt tgaacgaatc
     1741 ggtctgccgg aaggccgctt ccacctcgct caggccgccc tctaccttgc aaccgcgaaa
     1801 aagtccaact cgacgatggg gttcttcgac gcgttgaaaa aagtcaccga ggaagctcaa
     1861 cgcgaagttc ctaaccacct ccgcgacaac agccgcgacg ccaaggggtt cgggcacggt
     1921 gaaggatatc tctacccgca cgcctacagc gggcactggg tcgcccagca gtacctgccg
     1981 gcagccttgc aggggaagat cttttacaat ccgggcgacc aggggtacga ggcgacaatc
     2041 gcttccgagg tccaagggcg gcgggaggaa cagctggaat cgatccaaag cgacgtgttt
     2101 gtcgagcacc tctcctattc tcccccttcg tcggttgagg caatatggcg agaacgatcg
     2161 gcgggtgaac gatcgacgat tctgagggcg atcaggaagg agctctttcg cgaacatacg
     2221 gttcgccgaa gcgatcgggt tttcatcgcc aacgcgggca gcggctttct tctctgggaa
     2281 gcactgcgcc tgaccccgga gggcttgtgc gtagcccagg tgtccacgaa acagcacgag
     2341 gaagtgatca accactatgc aaaaacgctc gaaatgctgg aacggccgca cgttcacatt
     2401 ggagacccgg cctcggtcct ccgctcctta catgaacgac tcctctttga acacatctac
     2461 ggacgaaaca tcctctcccg cttgcatgcg gagaaagaaa tattggaact tttttttgag
     2521 aaactggtga aaggcggcac cctgaacatc gcccaaagca tcccctcccg ttcctcccga
     2581 ttaagcgagt tctgcgatca tccgctgctc aaagaagcgg aaaaagtcat ctatgccgat
     2641 gaaaccaacc ccctgatcaa ctgggacgag aaggatctgg aagaagcagc caaatcgtac
     2701 ggattccaag gaaagacgga agtgctctca ttcaccgaaa accgggtctt cacctcggat
     2761 cagcttgagg gatatctgaa gaactcctat ctgcccgccc tcaagaacga ggatccctcc
     2821 ttcgtcgttt cagtgatcaa ggctcttgag aagaagagcc tcgcctaccg tcaaagcatc
     2881 gcgatcctgc gccttgccaa ggattgaaca a
//
LOCUS       DAOSLI010000118         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_30770_length_2834_cov_22.199712, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000118 DAOSLI010000000
VERSION     DAOSLI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30770_length_2834_cov_22.199712"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="PLF67_09400"
     CDS             <1..1404
                     /locus_tag="PLF67_09400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE90264.1"
                     /translation="FILKTIFDDAPIGMSRTDPKTGKVLQINDAYAHFLGYEKEEIIG
                     RTWKEFTLPDEQNESDKVDFILVSGSKNQITLDKRYRTKDGRIVSGNLTVSNLSVGKY
                     SEEMLGMMVDITDRKKYEERLLRLSTHDPLTNLYNRNHFEQRVEEFVVPKDQTVAVLL
                     AEVNGLEILNEAFGQNEGNRLLKKIAKILQASTEETDYLARVGGDEFAVVLTGRSRHD
                     YQAIAKDITEELSLVQMPGGMDMIVSVVHAVVDEGDRTLHKAIEEAGKALKTRKLFEE
                     APIKENLVQTVMNALHVRNKPEEHHSRRVSFLSEQLGRVIGLSENEITQLRLAGLFHD
                     IGKIAIAEEILNKHGKLTAEEFSEIQRHVEIGYRLLSSVEGMAPIAHVILSHHERIDG
                     EGYPRGIKGEAIPLQARIISIADAYDAMTSSRTYKQQISSQEAARELKVCSGTQFDTD
                     LARHFIEGVLFLPYDEL"
     gene            1438..2085
                     /locus_tag="PLF67_09405"
     CDS             1438..2085
                     /locus_tag="PLF67_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOE90265.1"
                     /translation="MAVITISRQIGSSGTYIAGELAQNLNLAYADKAVITAVMEEYGF
                     SNFEKVYEERTGFWERYDEPRQKLVRFLNATMYALAKTQDIVILGRGGFGLFQQFTDV
                     LNVRIKAPLALRLERKMKEYGLSEETMLGALKEADLVRASFVEHDLGFDRNNAELFDL
                     VIDTGIVSPATAVDWIAEAYRHLTANPRIDGEFSTKNLMVDEILLKTVTEYLKKH"
     gene            2186..>2834
                     /gene="argC"
                     /locus_tag="PLF67_09410"
     CDS             2186..>2834
                     /gene="argC"
                     /locus_tag="PLF67_09410"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOE90266.1"
                     /translation="MIRCGVIGATGYAGSILVTLLVGHPEVEPVYLASHSYVGKRYSD
                     IYPSMAGHCDLILREEDLEEASTLCDVLFLALPHTLASQKVTQKILDRTVVIDLGADY
                     RLHDVDVYESWYQCEHGSKELVSQAVYGLCELHRGAIREANLIANPGCYPTCSILTLA
                     PLLGEGLVDPSSIIIDSASGVTGAGRSEKTANLFSEINESYKAYGVTNHRHTVEIE"
BASE COUNT          694 a          774 c          797 g          569 t
ORIGIN      
        1 ttcatcctga agacgatctt tgacgatgct ccgatcggca tgagccgaac ggatccgaaa
       61 acgggaaagg tgcttcagat caacgacgcc tacgcccatt ttctggggta tgagaaggag
      121 gagatcatcg ggcgtacctg gaaggaattt accctccccg acgaacaaaa tgaaagcgac
      181 aaggtggatt tcattctggt gagcggatcg aaaaaccaga tcaccctcga caagcggtat
      241 cggacgaagg atggccggat cgtcagcggc aacctcaccg tctccaacct ctcggtcggc
      301 aagtattccg aagagatgct cgggatgatg gtggacatca ccgaccggaa gaagtatgaa
      361 gagaggctgc tccgcctttc cacccatgac cccctcacca atctctacaa ccgaaaccat
      421 ttcgaacaga gagtcgagga gttcgtggtt cccaaggatc agacggtggc ggtgttgctg
      481 gccgaggtga acgggttgga aattctcaat gaagcgttcg gccagaacga aggaaaccgc
      541 ctgctgaaga agatcgcgaa gatccttcag gcctcgacgg aagagaccga ctacttggcc
      601 agagtcggcg gcgatgagtt tgccgtggtt ctcaccggcc gctcccggca tgactatcag
      661 gcgatcgcca aggacatcac cgaggagctc tccctcgtcc agatgcccgg agggatggac
      721 atgattgtca gcgtcgtcca tgccgtggtc gatgaagggg atcgcaccct ccacaaagcg
      781 atcgaggaag cgggaaaagc cttgaagacc cgcaaactgt ttgaggaagc cccaatcaag
      841 gagaacctcg tgcagaccgt catgaacgcg ctacacgttc ggaacaagcc ggaagagcac
      901 cacagccgaa gagtctcctt cctcagcgaa cagctcggac gggtgatcgg gctaagcgaa
      961 aacgagatta cccagcttcg cttggcgggc cttttccatg atatcgggaa aattgcgatc
     1021 gctgaagaga tcctcaacaa gcacggcaag cttaccgccg aggaattcag cgaaatacag
     1081 cgccacgtgg agattggcta ccgcctcctc agcagcgtgg aaggtatggc acccatcgcc
     1141 cacgtcatct tatcccatca tgaacggatc gatggggagg gatatccgcg gggaatcaaa
     1201 ggggaggcga tcccccttca ggcccgcatc atctcgatcg ccgatgccta cgatgcgatg
     1261 acttccagcc gaacctacaa acagcagatc agcagccagg aggcggcgcg ggagctcaaa
     1321 gtatgcagcg gcacgcagtt cgacacggat ctcgccagac acttcatcga aggggttctc
     1381 tttcttccct atgatgaact ctaggctgta gattgtacga ttgaaacgga ggtgcatatg
     1441 gcggtcatca cgatatcgcg acaaatcgga agttcgggca cctacatcgc cggcgagctt
     1501 gcacagaacc tcaaccttgc ctacgccgac aaggcggtca tcacggcggt gatggaggag
     1561 tacgggttct cgaacttcga aaaagtctac gaagaacgaa ccggcttctg ggagcgctat
     1621 gacgagccgc gccaaaagct tgtccggttt ctcaatgcaa ccatgtatgc gcttgcaaag
     1681 actcaggata tcgtcatcct cggccgaggc ggcttcgggc tctttcagca gttcaccgac
     1741 gtgttgaacg tccggatcaa agcgcccctg gccctgcggc ttgaacgcaa gatgaaagag
     1801 tatggcctca gcgaagagac gatgctcgga gctcttaaag aagcggatct tgttcgggca
     1861 tcctttgtcg aacacgacct cgggttcgac cggaacaacg ccgagctctt cgatctggtc
     1921 atcgacacgg gaatcgtttc tccggcgacg gcggtggatt ggattgccga agcatatcgc
     1981 cacctcacgg cgaatcctcg gatcgacggt gagttttcca ccaaaaatct gatggtcgac
     2041 gagatcctcc tgaagacggt caccgaatac ctgaaaaaac actgacatac atatttatgc
     2101 aaagaatccg caaattatat gcataagtac tggtaatatt tttcattttc tgtataatta
     2161 taccaatctt gatgaggagg ttggtatgat tcgctgtggg gttattggtg cgaccggcta
     2221 tgcgggctcg attcttgtca cgctgctggt cggccatccc gaggtcgaac cggtctatct
     2281 tgcttctcat tcctatgttg gaaaacgata tagcgatatc tatccctcca tggccgggca
     2341 ctgcgatctc atccttcggg aggaggatct tgaagaagcg agcaccctct gcgacgtgct
     2401 ctttctggcg cttcctcata ccctcgcttc gcaaaaagtg acacaaaaga ttttggatcg
     2461 aaccgttgtc attgatctcg gagcggatta ccgcttgcat gacgtggatg tatacgaatc
     2521 gtggtaccag tgcgagcatg gatccaagga gctggtaagc caggcggtct atggactctg
     2581 cgagctgcac cgcggggcga tcagggaggc aaacctcatc gccaaccccg gctgctatcc
     2641 gacgtgttcg atcctgacgc ttgccccgct gctcggagag ggactggtcg acccctcctc
     2701 gatcatcatc gacagcgcca gcggggtgac gggggcggga cgctccgaaa agacggcgaa
     2761 cctcttctcg gagatcaacg aatcgtacaa agcgtacggg gtgacgaacc accgccacac
     2821 ggtggagatc gagc
//
LOCUS       DAOSLI010000119         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_31097_length_2811_cov_17.988026, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000119 DAOSLI010000000
VERSION     DAOSLI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31097_length_2811_cov_17.988026"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..858)
                     /locus_tag="PLF67_09415"
     CDS             complement(262..858)
                     /locus_tag="PLF67_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE90267.1"
                     /translation="MLLLNEIRDTASGHPSCPSVINPYFATIIQGMKQEAGARARRYE
                     GLKSTIEKAGATLHLYTTEVSSFVELNTIDIEHQSGRTLANRCMNQSPEVTAMVAYGV
                     IDAIRDAGKEIPKDMSVCGFENIYPSSFNGVKLTSVKHCIMEGGRSAVRLLCEKIEPN
                     SNRTVERMAVTRVEYENKLVVRSSTGKPNTQQGEQALA"
     gene            881..1918
                     /locus_tag="PLF67_09420"
     CDS             881..1918
                     /locus_tag="PLF67_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE90268.1"
                     /translation="MELLKTLEFFFFPMHGIAMGIKEIAKEANVSVATVSRVLNGTKF
                     VSEELQRKVLEVIERQGYVANHVARSMVLKKTFTVGLIMPVVSELYHQMIFTHAEFIL
                     EEAGYKVLVCRVKDTAQSVQSYLDLLLENRVDGMILMHETSNPKVLEQLKKSQIPIVL
                     TGIDIPGLSFPQVRIDDYQAAYDGTKYLLSHGRKRIALIGGWGYSVGDKRLEGYKTAL
                     SEAGLEIDSSLVIPGFYTMQSGSQAVNYLIRERIKFDAVFALSDEMAVGVMKELLEQG
                     KEIPSDVAVLGFDGIELGRYTNPTLATVKQPIEQIGELAAERLLAMLNGEESEDSLIL
                     PHSISSGSSVG"
     gene            2124..>2811
                     /locus_tag="PLF67_09425"
     CDS             2124..>2811
                     /locus_tag="PLF67_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOE90269.1"
                     /translation="MKLTILGAASPRFPLLLNSLLKRENLSIDRVCLFDLDREKLGLL
                     SRTLLPEIMTHATGRFDLSAAATIEEAFEGADYVFSSIRVGGQHRRALDETHAVELGE
                     IGQETVGIGGFLSALRTIPVMLEHVDFMQRYAPGAMLINFTNPSGIVTQAVHSLSGFS
                     GIVGICDAPQMVATYTAEIYDVDPSEVQILSYGLNHYGWVYSVQVQGIEVLDQLLDCR
                     REEFFAKEPFY"
BASE COUNT          625 a          707 c          816 g          663 t
ORIGIN      
        1 aatcacgcaa gtcttccacc cgcctttatg ggcatatgat tttcctgttg aagggatggt
       61 acctaatgga tgtttatgtt tcattcatgc aaccgccggc atcatccggt gtttcatatt
      121 ttaccgtttt tctatttttc acggccccat tgtacggatg caatgttaaa tcaaaaaggg
      181 aagtcatgtt aaatgcaagt aaagcggaaa ccggtctgtt tccctgccgt tcggaattga
      241 tgatcgggct gcaggccgga atcacgcgag ggcctgctcg ccttgctgag tgttcggctt
      301 tccggttgaa gaacggacga ccagcttgtt ttcatactca acccgggtaa ccgccatgcg
      361 ttccacggtc cgattggagt tcggttcgat cttctcgcac agaagacgga ccgcgctgcg
      421 gcccccttcc atgatgcagt gcttaacgga agtgagcttc actccgttga atgatgaggg
      481 atagatgttc tcgaacccgc agacgctcat atccttcgga atctctttcc cggcatcccg
      541 gattgcatcg atgaccccat aggcgaccat ggcggttact tcgggacttt ggttcatgca
      601 tcgattggcg agggtccgtc cgctttggtg ctcgatgtca atggtattga gctcgacgaa
      661 ggaagagacc tccgtcgtat agaggtggag cgtcgcaccc gctttctcga tcgtggactt
      721 caacccctca tatcgacggg ctcgggcgcc ggcttcctgc ttcattccct ggatgatcgt
      781 cgcgaagtac gggttgatga ccgaggggca ggacggatgt cctgaagccg tatctcggat
      841 ttcgttgaga aggagcatgt ggacgggtac gatcccgagt atggaacttt tgaaaactct
      901 tgagtttttc ttcttcccga tgcatggtat tgctatggga atcaaagaga tcgccaaaga
      961 ggccaacgtc tccgtagcga ccgtttcacg ggtgttgaac ggaaccaaat tcgtgagcga
     1021 agagcttcaa aggaaagtcc ttgaggtgat tgagcgccaa ggatatgtcg ctaaccatgt
     1081 ggctcggagc atggttctca aaaagacgtt caccgtgggc ctgatcatgc ccgtcgtgtc
     1141 ggagctctac caccagatga tcttcaccca cgccgaattc attctcgagg aagccggata
     1201 caaggtgttg gtctgtcggg tgaaagacac tgcgcaaagc gtccagtcct acttggacct
     1261 cctgctggaa aaccgggtgg atggaatgat cctgatgcac gagaccagca accccaaggt
     1321 gctcgagcag ctcaagaaaa gccagatacc gatcgttctc acgggtatcg acatcccggg
     1381 cctttccttt cctcaggtgc ggatagatga ttatcaggcc gcatatgatg gaacgaagta
     1441 tctgctctcc catggcagga agcggatcgc cctgataggg gggtgggggt attcggtcgg
     1501 agacaagcgg cttgaagggt ataagacggc gctgtcggaa gcaggtcttg aaattgattc
     1561 ctcgctcgta atcccgggat tctacacgat gcagtcgggc tctcaggcgg tgaactacct
     1621 gatcagggaa cggatcaagt tcgatgccgt gttcgcgttg agcgacgaaa tggcggtcgg
     1681 ggtgatgaaa gagctgttgg agcaggggaa ggagattcct tcggacgtcg ccgtcctcgg
     1741 gtttgacggg attgagctgg gccgatacac caatcccaca ttggcgaccg tcaagcagcc
     1801 gatagaacag ataggagaat tggccgccga gcggctgctg gcgatgctca acggggagga
     1861 gagcgaagat tcgctcattc ttccgcacag catctcttcc ggcagcagcg ttggatgatc
     1921 ggttgagagg attcggtcaa atacgcaaag aggaaagaaa taatttgaca ggaaatcgtt
     1981 ttcctattac cctgtgacca aagccgggga acagcatatg atctccggaa tcaccgtcgg
     2041 agcgttgaag gagtaggaga gaaggtgttt cttatcggaa aaacacttgt tcatgaacct
     2101 ggaacggtac aaggagggaa gccatgaagc tcacgattct cggagcggcg agtccccggt
     2161 ttcccctgtt gctcaattcc ctgctcaaac gggagaatct ttcaatcgac cgagtgtgtc
     2221 tcttcgacct cgaccgggag aagcttggcc tgctgtcaag aacgcttctg cctgagatca
     2281 tgacccatgc aaccggacga ttcgaccttt ctgccgccgc cacgatcgag gaggcgttcg
     2341 aaggagccga ctacgtcttc tcttcgatcc gggtcggtgg gcagcaccga cgggcgctcg
     2401 atgaaacgca tgcggtcgaa cttggcgaga tcgggcagga gaccgtcgga ataggcgggt
     2461 ttctgagcgc cctcaggacg attccggtca tgctggagca tgtggatttc atgcagcgat
     2521 acgcccctgg agccatgctg atcaacttca cgaatccatc ggggatcgtc actcaggcgg
     2581 ttcactcgct ttccggattt tccgggatcg tgggtatctg tgacgcccct cagatggtcg
     2641 ccacctatac ggcggaaatc tacgatgtcg atccgagcga ggtgcagatc ctttcctatg
     2701 ggctgaacca ttatggatgg gtgtattcgg ttcaggttca aggcatcgag gttctcgatc
     2761 agctgcttga ttgccgaaga gaggagtttt tcgccaagga gcccttctat a
//
LOCUS       DAOSLI010000120         2751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_31955_length_2751_cov_25.429896, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000120 DAOSLI010000000
VERSION     DAOSLI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31955_length_2751_cov_25.429896"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLF67_09430"
     CDS             complement(<1..757)
                     /locus_tag="PLF67_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE90270.1"
                     /translation="MQDIRRSIFAWAWYDWANSAFATTVMAGFFPVFFTAYWAVGSSA
                     EQGTFFLGVANSLGSFIVALLAPFLGAIADWGSYKKRILTFFTFLGAVMTGSLFFLKM
                     GSWVLAVLYYSLALVGFSGANTFYDGLLPSVASEKKVDYVSSLGYSLGYIGGGLLFLV
                     NVLMYLNPHWFGLPDEATAIRVSFVIVGIWWLVNTIPLWLWVKEEKVEGELTVRESVK
                     KGLRLTVNTIKSFRQLKTVATFLLAYWLYIDGVD"
     gene            879..2183
                     /locus_tag="PLF67_09435"
     CDS             879..2183
                     /locus_tag="PLF67_09435"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOE90271.1"
                     /translation="MVVCKFGGSSVADAAQLRKIKQIIDSNPNRTIVIVSAPGKRRSD
                     DEKVTDMLYGCAALVDQGKPCKPIFEKVVERYSTILDDLKIDRTEFDPLFRCIEAEIN
                     EGAGSHYAASRGEYLSAKVVAAFFGWEFLDTQDLITIESDGTVASSSYEKIARAIDPK
                     KRYVVPGFYGSDSEGIIKTFSRGGSDITASVFARAVDADVYENWTDVSGIYAIDPRLT
                     DKAKVIQELTYQEIRALAGVGANVFHEEAIAPLYHLSIPINVKNTNDPTAEGTWIRAT
                     RDYTNTPLAGVSAKAGYTRIVVQKLLLLKKVGIRQGMLTMLHVFGIHPTFTSSGIDSL
                     AWYFDSSQANEQIVTALCVRLKDEFELDEVTVTTDIAITGVVGEGSSRLEGLTSKATG
                     ALAEEGIELMFLSVGSSPNTLLLGVPQNKQKEAAIALYSALF"
     gene            2193..2702
                     /locus_tag="PLF67_09440"
     CDS             2193..2702
                     /locus_tag="PLF67_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOE90272.1"
                     /translation="MIATETLLLSLMIFSARVVDVSLSTIRQVMIIRGKRAITFGIAF
                     IENLIWVFAVSKVLQDLSEPITALAFALGFATGTFVGITLEGKLRIGEQVVKVFTSLG
                     DEVADHLRSNGYRLTEFMGKGRDGEVSLLFVQVRRRDAPKVLTLARELDPACYLVLED
                     IRAHQRATR"
BASE COUNT          686 a          681 c          731 g          653 t
ORIGIN      
        1 tgtcgactcc gtcaatgtag agccagtagg caagaagaaa ggttgcaacg gtcttaagct
       61 ggcggaagct ctttatcgtg ttgaccgtca gtcgcagtcc tttttttact gattcacgga
      121 cggtgagttc accctccacc ttttcttctt tcacccaaag ccatagtgga atcgtgttga
      181 ccaaccacca gatgccgacg atgacaaagc ttactctgat ggctgtcgct tcatcgggga
      241 gaccgaacca atgggggttg aggtacatca gaacattcac caaaaacagg agtcctccac
      301 cgatgtaacc gaggctgtac cccagtgaag agacgtaatc gactttcttc tccgaggcga
      361 cggatggaag aagcccgtca tagaacgtgt tcgctccgct gaagccgaca agtgcaaggc
      421 tgtaatacag gaccgcaagc acccacgagc ccattttcaa aaaaaagagc gatccggtca
      481 ttaccgctcc gaggaaggtg aagaaggtga ggattctttt tttatagctt ccccagtcgg
      541 cgattgctcc aagaaacgga gccagaaggg ctacgatgaa cgaaccgagg gagtttgcga
      601 ctcccaagaa gaaggtcccc tgttcagcgg aggacccaac agcccaatat gcggtgaaga
      661 acacggggaa aaacccggcc atcaccgtgg tggcaaacgc actgtttgcc caatcatacc
      721 aagcccaggc aaagatcgat cggcgtatgt cctgcattat atacctcgtt gctgtcacga
      781 tagcattacc gttggacctt gtcagccctt tgttttcgtc ttgaacaaga acgttgcctc
      841 ctctacaata cgcccataaa ttcatacagg agtccgagat ggtagtatgt aaatttggtg
      901 gcagctcggt tgcagatgcc gctcaactga ggaagatcaa gcagattatc gacagtaatc
      961 ccaatcgaac gatcgtaatt gtctccgctc cgggaaagcg aagatccgat gacgagaaag
     1021 tcaccgacat gctctacggg tgcgccgcct tggtcgatca aggcaaaccg tgcaaaccga
     1081 tctttgaaaa ggtcgtcgaa cgctacagca cgatcttgga tgatttgaag atcgatcgga
     1141 ccgaattcga tcctctattt cggtgtattg aagcggagat caatgaaggt gccggcagtc
     1201 actacgccgc aagtcgggga gaatacctct cagcgaaagt ggtcgccgcc tttttcggat
     1261 gggagttctt ggatacacag gatcttatca ccatcgaaag cgacggaaca gtggcttcct
     1321 cctcctatga gaaaatcgcc cgggcgatcg atccgaagaa gcgctacgtg gtcccgggat
     1381 tctatggcag cgacagtgag ggtattatca aaaccttcag ccgcgggggc agcgatatta
     1441 ccgcaagcgt cttcgctcgg gcggtggatg ccgatgtcta tgaaaactgg acggatgttt
     1501 ccggaatcta tgcgatcgat ccccggctta ccgataaagc gaaagtaatc caagagctca
     1561 cctatcagga gatacgggct cttgcgggag tgggggcgaa tgtgttccat gaggaagcga
     1621 tcgctccgtt gtatcatctt tcgattccaa tcaatgtaaa gaatacaaat gatccgaccg
     1681 ctgagggaac ttggattcga gcaacccgtg attacaccaa cacaccgctt gcgggagttt
     1741 ccgccaaagc gggatatacc cgcatcgtgg ttcaaaaatt gctgttgctg aagaaggtcg
     1801 gaatccgtca gggaatgttg acaatgctcc atgtctttgg aattcacccg acatttacat
     1861 catcgggaat cgattcgctt gcgtggtatt ttgactccag ccaagcgaat gagcagatcg
     1921 ttaccgccct ctgtgttcgc cttaaagatg agtttgagct tgacgaagta accgttacta
     1981 ccgatattgc aattacaggt gtggttggtg agggctcaag tcggttggaa ggactgactt
     2041 cgaaggccac cggagcgctt gccgaagagg ggattgaact aatgtttctc agtgttggca
     2101 gttcaccgaa tactctgctg ctcggagtcc cccagaataa acaaaaagag gctgcgattg
     2161 ccctctattc tgcgctcttt taaggaagtg tcatgattgc tactgaaacg ctattactct
     2221 ctttgatgat tttcagcgcc cgagtggttg atgtttcatt atcaacaatt cgccaagtga
     2281 tgattattcg aggtaaacgg gcgattacgt tcggaattgc ttttattgaa aacctgattt
     2341 gggtcttcgc cgtgtcaaag gtgctccaag acctttccga accgattacc gcattagcgt
     2401 tcgctctcgg ttttgctacg ggaacgttcg taggcatcac gttggaagga aagctgcgca
     2461 tcggcgaaca ggtcgtgaaa gttttcacct cccttggtga tgaggtcgcc gaccatcttc
     2521 gctccaacgg ctatcggctc acggaattca tgggcaaggg gcgggatggc gaggtgtccc
     2581 tgctgtttgt ccaagttcgg cgtcgggatg ctccgaaggt gttgactctt gcccgcgagc
     2641 tcgatcccgc atgctatctg gtccttgaag acattcgcgc ccaccagcgg gcaacaaggt
     2701 gattgaagat atatggatac aagagaaccg gccgatccgg ccggttcttt g
//
LOCUS       DAOSLI010000121         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_33708_length_2641_cov_20.108275, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000121 DAOSLI010000000
VERSION     DAOSLI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33708_length_2641_cov_20.108275"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..531
                     /locus_tag="PLF67_09445"
     CDS             304..531
                     /locus_tag="PLF67_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE90273.1"
                     /translation="MNKKKIVVLGGGYAGVHAAKTLNKAFKKHQDKVEVTLIDKKHHH
                     ILLTEPHEVAGARVSEDSARYRLTESLPGKK"
     gene            528..1619
                     /locus_tag="PLF67_09450"
     CDS             528..1619
                     /locus_tag="PLF67_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOE90274.1"
                     /translation="MNVVQDAIVEIDFKKQQLIGQQAKYGYDELIIGTGATTADFNIP
                     GVKEHAFYLRFLEEAIKIRCHIEETVAASHPFVRQVLLDYVRTGGKMLRPALVLIMST
                     LNGNNIDQDAINTAAAVELIHLASIVHDDIIDGAPIRRGKKTLSKQAVLAGDLLLSKA
                     LTLTGRLDTKTNRVAMARLCESELEQDANVGNFFIIRTACTRRIAGKTASLFALSCYL
                     GSALATEDQLVRMRAHRLGYALGMAFQIQDDNLDYDGEAVLPGKEIAWDLKNGIPTLP
                     LLLALQEEKKAGSALLHSYLHEPKLPLSKKNINTILSLVDHYRGVEKAQKIEQQYRKR
                     VERDIEALDTPEIERMLHSLISRLSLRRN"
     gene            1622..1894
                     /locus_tag="PLF67_09455"
     CDS             1622..1894
                     /locus_tag="PLF67_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90275.1"
                     /translation="MDEYDFSGEQRVFDNAYDLMIHHLELRARTTPLSIHDLEGELHH
                     LTIYEGQDWDGRGSLKAAEIDGQIQAYITMINRMKQDRNDKDVHSK"
     gene            complement(2000..2446)
                     /locus_tag="PLF67_09460"
     CDS             complement(2000..2446)
                     /locus_tag="PLF67_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE90276.1"
                     /translation="MSNVQSILDQKGSSVFSITPDDVLAHALLQMNEQKVGALLVLNE
                     TGDIKGILSERDIVRYFSRRMQHINTTEIKVKDVMTAGVTCVKRHQSLEDCLQLMTVG
                     RFRHLPVVDDTGKVMGMVSIRDVVKAVLDAKDFQIGEMEQYITSAY"
BASE COUNT          685 a          668 c          637 g          651 t
ORIGIN      
        1 ctctatctat aaatatgtta tgttggaaag acgattggca tcaaccatat aaattattct
       61 ttatcgattt cacgtttgat ttcaatccgt attcctgaat acattgaaga tgctcttttt
      121 gttgaaaaaa caaaggcatt tctcccgttt tgagctcttt tcaattgaac cccatcatat
      181 cgatataaaa caaagaattg cattatcgcc tgcacttgtg gtagagttac cacgcctata
      241 gaagatattg gtcaactaca tttggcaggg tcgaaaccct gttttcttaa gaacggtagt
      301 actatgaaca agaagaagat tgtggtcttg ggtgggggct atgccggagt tcacgcagcc
      361 aagacactga acaaagcgtt taaaaagcat caggacaaag tcgaggtgac gctcattgac
      421 aagaagcatc accacatcct gcttactgag ccgcacgagg tcgcaggggc ccgggtaagc
      481 gaagattcag caagatatcg tttgaccgaa tctttgccgg gaaaaaagtg aacgtggttc
      541 aggacgcgat cgtcgaaatc gattttaaaa agcagcagct tattggccag caggcaaaat
      601 atggttacga tgaactgatc atcggcaccg gtgccaccac tgccgatttc aatattccgg
      661 gggtaaaaga acatgctttc tatcttcggt ttttggaaga agcgatcaag atccgctgtc
      721 acattgaaga gacggtcgca gcttcccatc cctttgttcg ccaagtgctg ttggactacg
      781 ttcgaaccgg cggcaagatg ttgcgtcctg ccctggttct catcatgagc acgcttaacg
      841 gaaacaacat cgaccaggat gcgatcaata ccgctgcagc ggttgaattg atccacctcg
      901 cttcgatcgt ccatgacgat atcatcgatg gagctccgat ccgccgcggg aagaaaaccc
      961 tctcgaagca ggccgtgttg gcgggtgatc tcctgctttc aaaggcactc acattgaccg
     1021 gccgcctcga cacgaagaca aaccgggtag cgatggcccg cctctgcgaa agcgagcttg
     1081 aacaagatgc gaatgtgggc aactttttta tcatccgaac tgcctgcacc cgacgaattg
     1141 ccggtaaaac cgccagtcta ttcgccctca gctgttacct tggttcagcg ttggcaaccg
     1201 aagatcaatt ggtaagaatg cgcgctcatc gtctcggata tgcgctcggg atggcgtttc
     1261 aaattcagga cgataatctc gactacgacg gagaggcggt ccttccggga aaagagatcg
     1321 cctgggatct gaaaaacggg attcctacgc ttcctctctt gcttgcgttg caggaggaaa
     1381 agaaagccgg ttccgctctc cttcattcgt atctccatga accgaagttg ccgctctcaa
     1441 agaagaacat taacaccatc ctttccttgg tggatcacta tcgaggtgtt gaaaaagccc
     1501 aaaaaatcga gcagcagtat cgcaagcggg ttgagcgtga tattgaagct ttggataccc
     1561 ccgaaattga gcggatgctc cacagcctga tcagccgtct ttcgttgagg aggaattaga
     1621 attggacgaa tatgattttt cgggtgagca acgggtattc gacaacgcct atgatttgat
     1681 gattcaccac cttgaattga gagcccgaac caccccctta agcatccatg atctggaggg
     1741 ggaactccat cacttgacaa tctatgaggg acaggactgg gatggtcggg gcagcctgaa
     1801 agccgccgag atcgacggtc agatccaagc gtatatcacg atgatcaatc ggatgaaaca
     1861 ggaccggaat gataaggatg tccatagtaa atgagggctg agcaaccgcc tcagcccttt
     1921 ttattttcat aatcgacaca atcgcatcta cgctgctcaa tcaccgcgtt gagcttgtga
     1981 tttccattct actcgcatcc taataggcgc tcgtgatgta ctgctccatc tcgccgatct
     2041 ggaaatcctt ggcatccaga accgctttca cgacgtccct gatcgatacc atccccatga
     2101 ccttcccggt gtcgtcgacc accggcaagt gcctgaagcg gccgacggtc atcagctgaa
     2161 ggcaatcctc aaggctctgg tggcgtttca cgcatgtcac cccagcggtc atcacatcct
     2221 tgactttgat ctccgtggtg ttgatgtgct gcatccggcg ggagaaatag cggacaatgt
     2281 cgcgctcgga aagaatcccc ttgatatccc ctgtctcatt gagaaccaaa agagctccga
     2341 ctttttgttc gttcatctgt aaaagcgcgt gagccaacac atcatccggt gtaatgctaa
     2401 agactgaact gcccttctga tccaaaatag attgtacgtt tgacatctgc tacctccgct
     2461 agtgattctc ttcctatcgt acatcaccaa atacccaaac gcaatgattt ctactgtttt
     2521 cgtgcatcga ttagatggtt gatcgttcga tccagaacgg aggcaaggct ccgggcgccg
     2581 gtctcatcat catcaccgaa cagacacgag gccatatggg agggaattga gcgggcttct
     2641 t
//
LOCUS       DAOSLI010000122         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_34186_length_2613_cov_27.366693, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000122 DAOSLI010000000
VERSION     DAOSLI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34186_length_2613_cov_27.366693"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLF67_09465"
     CDS             complement(<1..236)
                     /locus_tag="PLF67_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90277.1"
                     /translation="MNRYLRFITRRERITTPLWILSIVSSLLFFTALYPTLIPTNEEA
                     LALARTMQNPAMVAMMGPIYGLDAISQAIVMSQET"
     gene            complement(233..919)
                     /locus_tag="PLF67_09470"
     CDS             complement(233..919)
                     /locus_tag="PLF67_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE90278.1"
                     /translation="MAVIVINDVSKHFGKTKALDGVTLTIEENTIHGFLGPNGAGKTT
                     MIRILLGIIRPTRGEASIFGLDVYKDRIPIHSRLAYVPGEVNLWPNFTGGEVIDLLIG
                     RERLDRKRIDYYLDLFELDPSKKCKTYSKGNRQKVALVAAFASPAEVFILDEPTSGLD
                     PLMEQRFQQVIKAKHGEGKTIFLSSHILSEVQKIATAVSIIKQGRIEETLLIDEHTDL
                     ERFFHESNGL"
     gene            1001..>2613
                     /gene="nadE"
                     /locus_tag="PLF67_09475"
     CDS             1001..>2613
                     /gene="nadE"
                     /locus_tag="PLF67_09475"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOE90279.1"
                     /translation="MIDGLITVAALSPGLRVADPSFNGKEIVAGIEEAAKQGVCLLVL
                     PELCTTGSTCGDLFYSQTLLKASDDAVRKIVGSTTSLQLIVVFGAPVAFGSNLYNASI
                     VAYDGQILAIVPKPAAGVFASYEGEPLSVELFDAYTYFGNGIRIAPSAIPHFVLAIDD
                     FKTPSGATVVASPISEAELIGRSTKRRLLAQGFTISNTCALVQANSGEGESTTDSVYL
                     ADALIVERGKILSESGGMSGKLVIGQIDLQVLVHQRRLEKTFPLYEEGCLEIPCDLVH
                     APSSLIRHIDPAPFVPHDPVRLKERCEEVLTLQALGLKKRLEHIGIKRVILGLSGGLD
                     STAALLAVIRTFDVMSLDRGGIIAVSMPGFGTTSRTKSNAQALAEALSVTFFTIPIEK
                     AVTQHFKDIDHDPTVLDITYENAQARERTQVLMDLANKHGALVIGTGDLSELALGWTT
                     YNGDHMSMYAINSSVPKTLLRPILAHEANEADEDLQAVLLDILATPVSPELLPPSGDG
                     AISQKTEAVIGPYELHDFFLYYTLRWGFGP"
BASE COUNT          543 a          737 c          735 g          598 t
ORIGIN      
        1 gtctcctgac tcatcacgat cgcctggctg atcgcatcaa gtccgtagat cggtcccatc
       61 atcgccacca tcgccgggtt ctgcatcgtg cgggcgaggg ctaaagcctc ttcattcgtc
      121 gggatcaggg tcgggtagag ggcggtgaaa aagagaagcg aggaaacgat cgagaggatc
      181 cagagagggg tcgtgatccg ttcccgccgg gtgatgaagc gaaggtatcg gttcatagcc
      241 cgttgctctc atggaagaac cgctcaagat ccgtatgctc atcgatgagg agggtctctt
      301 cgattcggcc ctgcttgatg atggagaccg cggtcgcgat cttctgcact tcgctgagga
      361 tgtgcgaaga aaggaagatc gtctttccct caccgtgctt ggcttttatt acctgctgga
      421 agcgctgctc catcagagga tccaacccgc tggtcggctc atcgaggatg aacacctccg
      481 caggggaggc gaacgccgcc accagggcga ctttctggcg atttcccttt gagtaggtct
      541 tgcacttctt cgaagggtcg agctcaaaga ggtcaagata gtaatcgatc cgtttacgat
      601 caaggcgttc cctgccgatg agcaggtcga tcacctctcc tccggtgaag ttcggccaga
      661 gattgacctc cccggggaca taggcaagcc ggctgtggat cgggattcta tccttgtaca
      721 catcaaggcc gaagattgat gcctcgcccc gagtgggtcg gatgattcca aggaggattc
      781 ggatcatcgt ggtcttgccc gccccgttgg gaccgagaaa tccgtgaatc gtgttttctt
      841 cgatcgtcag ggtcaccccg tcaagggctt tggtttttcc gaagtgcttg gaaacgtcat
      901 tgattacaat cactgccatg gcattagaat accacacaac cgcctcttgc ccaccactat
      961 tcttgggcgg tacactgagg gtgtggagag gaggtcgacg atgattgacg gattgattac
     1021 ggttgcggcg ctttcgcccg ggctcagggt tgcagatcct tcgttcaacg gcaaagagat
     1081 cgtcgccgga attgaagaag cggcaaaaca gggtgtttgc cttctcgtac tacccgaact
     1141 ttgcaccacc gggtcgacgt gcggggatct cttctactcc caaaccctcc tcaaagcgag
     1201 cgatgatgcg gtaagaaaga ttgtcggctc tacaacctcc cttcagctga tcgtcgtctt
     1261 cggagcgccc gtcgccttcg gatccaatct ctataacgct tcgatcgtcg cctatgacgg
     1321 gcaaatcctt gcgatagtac ccaaacccgc cgccggcgtc ttcgcatcat atgagggcga
     1381 gccgctctcc gtcgagctgt ttgatgcgta cacctacttc ggcaacggga ttcgcatcgc
     1441 cccgtcggcg attcctcatt tcgttcttgc aatcgacgat tttaagacac cgagcggagc
     1501 tacggtggtc gcttccccga tcagtgaagc ggagctgatc ggtcgttcca ccaaacgccg
     1561 gcttcttgct cagggcttta cgatttccaa cacctgcgct ctcgttcagg cgaacagcgg
     1621 agaaggggag tcgacgaccg attccgtcta tcttgccgat gcgctgatcg tcgaacgggg
     1681 aaaaatcctc agtgagagcg gcggaatgag cggaaagctc gtaatcggac agatcgatct
     1741 tcaggtgctt gtccatcagc gtcggcttga aaaaaccttc cccctctacg aagagggctg
     1801 ccttgaaatt ccctgtgatc tggttcatgc tccatcctcc ttgattcgcc atatcgaccc
     1861 ggcgccgttc gttccccatg atccggtgcg gcttaaggag cgctgcgaag aagtgctcac
     1921 gcttcaggcc ctcggcctga agaaacgtct tgaacatatc ggcatcaaac gggtgattct
     1981 cggtctctcc ggaggacttg attcaacggc agccctgctt gccgtcatcc gcacgtttga
     2041 tgtgatgagc cttgaccgcg gaggcatcat tgcggtctcg atgccggggt tcggaacaac
     2101 aagccggacg aaatcgaacg ctcaagcttt ggcggaagcg ctctcagtca cgttcttcac
     2161 gatcccgatc gaaaaggcgg tgactcagca cttcaaggat atcgatcatg atccgacggt
     2221 gttggacatc acctatgaaa acgcccaggc gcgggagcga acacaggttt tgatggatct
     2281 ggccaacaaa cacggggcct tggtcatcgg caccggagat ctttccgagc ttgccctcgg
     2341 gtggacgacg tacaacggtg accatatgtc gatgtatgcg atcaacagct cggtgccgaa
     2401 gactctgctc aggccgattc ttgcccatga agcgaacgaa gccgatgaag atcttcaggc
     2461 tgttttgctg gacatccttg ccactccggt cagtcccgag cttcttcccc cgtccgggga
     2521 cggggcgatc agtcagaaaa cggaagcggt catcgggccg tatgagctcc atgatttctt
     2581 cctttactac accctgcgct gggggttcgg tcc
//
LOCUS       DAOSLI010000123         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_36615_length_2478_cov_11.640941, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000123 DAOSLI010000000
VERSION     DAOSLI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36615_length_2478_cov_11.640941"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2478)
                     /locus_tag="PLF67_09480"
     CDS             complement(<1..>2478)
                     /locus_tag="PLF67_09480"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOE90280.1"
                     /translation="EWIRALEEARGDEPKIIEEIGCTYLDVMRASLKSEEEPWFEEEK
                     PVVLVSPTWGSHGLLSRYGKAVLQALTEDKKYNIIVRPHPQSFIAEGKLMEELQTTFP
                     DSSNLRWDRRNSGLEAMGQADVMVSDFSGIIFDFLFLFKKPILTFKGIFDKRGRDAMD
                     VDREPWNVEILDRIGRTLGEEDLPHLSAIISATLQDPVSFEASFQEAQMGMDRYPGES
                     GRRGADFIERTLNTLPRTKEAISKPVSSEPQGWTGKIRAVVSTLFDPSFYLEAFFALV
                     LFYGYLLIGKRILVVDGFNYKFVTQGLPWVAKVLPLPLIGSVVLIWIRERGACSFVRK
                     REPFSLKELWLLLFPMAPITQYVIANQDILLFGDSLAVLGFFLALSFGMVILVPYFLS
                     PLMRKHFTVTIGLALAFHLFNMANFIGIFGMGRKRIQVPLFLAIALMIFVLYGINKKG
                     LYVFSVLFFVVTLGSAVYSTLGIGEERVTTQSGKVAVVAGRSAQKTPDVYLLIYDSYP
                     NEETLEFYGIDNRQMYESLLEKGFAIYDGTYSVGPISLESIAHVMDFEQAGWSTNLRK
                     VLAQDANGLKIFKEAGYMNYSIMPNDYMVRGVRIDPSVHDSYFPNPEDGDVNIRSSRI
                     LISAISEGMFRFDAAFGHTTEEEFTQEKRNFLRVQSQQPRFLYTHENVPGHTTDIGVL
                     ADNETEMWADRLPWANDIIEDDLAIALEHNPDALIIVAADHGPYLTKNGKDLNVPAYS
                     LGDITRYDVQDRYGTLLAIRWPEEGYETRYDIRILQDVLPAVFAYIYDDDVLFDRLRM
                     ERKTLYPYVTGGVVVEDGIVVGGADDG"
BASE COUNT          586 a          687 c          634 g          571 t
ORIGIN      
        1 ttccgtcatc cgcgcccccg accacgatgc cgtcttcgac cacgactcct cccgtcacat
       61 acggatagag cgtcttccgt tccatccgga gccggtcgaa caacacgtca tcgtcataga
      121 tataggcgaa cacggcggga aggacatcct gaagaatcct gatgtcgtat ctggtctcgt
      181 atccttcttc cggccagcgg atcgccagaa gcgttccata tcggtcttga acatcgtacc
      241 gggtgatgtc accaagcgaa tatgccggaa cgttgaggtc tttgccgttc ttggtcagat
      301 acggcccatg atcggccgcc acgatgatga gggcatcggg gttgtgttca agggcgattg
      361 ccagatcgtc ttcgatgatg tcattcgccc agggaagacg atccgcccac atctcggttt
      421 cattatccgc caagaccccg atgtcggtcg tgtgccccgg aacattttca tgagtatata
      481 aaaagcgcgg ttgctgtgat tggacacgca ggaaattcct cttctcttgg gtgaactctt
      541 cctcggtcgt atgaccaaat gcggcgtcga agcggaacat cccctccgag atcgccgaga
      601 tgagaatcct tgagctcctg atattcacat caccgtcttc cggattgggg aaataggaat
      661 catgcacgga ggggtcgatc ctcactcccc tgaccatgta gtcgttcggc atgatcgaat
      721 agttcatata tccggcttcc ttgaaaatct tcagcccgtt tgcatcctgt gctaggactt
      781 ttcgcaggtt cgtcgaccaa cccgcttgtt caaaatccat gacgtgtgca atggactcaa
      841 gggagattgg accgacgctg taggttccat catagatggc gaaccctttt tcaagaaggc
      901 tctcatacat ctgtcgattg tcgatgccgt agaactcgag ggtctcctca ttgggatagg
      961 aatcgtagat cagcaggtac acgtccgggg tcttttgagc cgaccgtccg gcgacgaccg
     1021 ccactttccc gctttgagtc gtcactcgtt cttctccgat ccccagcgtc gaatagaccg
     1081 cgcttcccag cgtgacgaca aagaacagaa ccgagaacac atacaagccc ttcttgttga
     1141 ttccatagag gacgaagatc atcagcgcga tcgccagaaa gaggggaacc tgaatccgtt
     1201 tccttcccat tccgaagatg ccgataaagt tcgccatgtt gaacagatgg aaggccaaag
     1261 caagaccgat cgtgacggtg aagtgcttcc gcattaaagg acttaggaag tagggaacaa
     1321 gaatcaccat gccaaaggag agtgccagga agaatcccaa gaccgcaagc gagtccccga
     1381 agagaaggat gtcctggttg gctatcacat actgggtgat cggggccatc ggaaagagca
     1441 gaagccaaag ttccttaaga gaaaacggct ctcttttccg gacaaaagaa catgctcctc
     1501 tttcccggat ccagatgagg acaaccgaac cgatgagtgg aagcggaagg actttggcca
     1561 cccacggcag tccctgagtg acaaacttgt aattgaaccc gtcgaccacc agaatccgct
     1621 ttccgatgag caggtatcca tagaagagca ccagagcgaa gaacgcttcc agataaaaag
     1681 aaggatcgaa cagcgttgat acgaccgccc ggatcttgcc tgtccatccc tgaggttccg
     1741 aagaaaccgg tttggaaatc gcttctttgg ttcgaggaag agtgttcagc gttcgttcga
     1801 tgaagtccgc tcctcttctt ccgctctccc ccggatagcg atccattccc atctgagctt
     1861 cttggaacga tgcttcaaac gagacggggt cctgaagggt cgcactgatg atcgcagaga
     1921 gatgcggaag atcctcttca ccaagcgttc ttccgatccg gtctaagatc tcgacgttcc
     1981 acggctcgcg atcgacgtcc atcgcatcac gaccgcgctt atcgaagatt cctttgaagg
     2041 tgagaatcgg cttcttgaag agaaagagga aatcgaagat gattcccgag aagtcgctga
     2101 ccatgacatc cgcctgaccc atcgcctcca gaccgctgtt tcttcgatcc cagcggagat
     2161 tcgaggaatc ggggaatgtg gtttgaagct cttccatcag ctttccttcg gcgatgaagg
     2221 actgggggtg cggccggacg atgatgttgt acttcttgtc ctcggtgagg gcttggagaa
     2281 ccgctttccc atatctggag agcaggccgt ggcttcccca cgttggagat accagcacca
     2341 cgggcttctc ttcttcaaac caaggctcct cttcactctt cagcgatgct ctcatcacat
     2401 caagataggt gcacccgatc tcttcgatga tcttcggttc atctcccctt gcctcttcaa
     2461 gggcccggat ccattcaa
//
LOCUS       DAOSLI010000124         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_37778_length_2419_cov_18.219966, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000124 DAOSLI010000000
VERSION     DAOSLI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37778_length_2419_cov_18.219966"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLF67_09485"
     CDS             complement(<1..358)
                     /locus_tag="PLF67_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE90281.1"
                     /translation="MIEDENHALVIDTGPEFRLQALKHKIARLDAVFYTHDHADHING
                     IDDLRSFSRHGPLRVYASRNVVETIRVRFSYALDADVVAGGRPHLLLTVIKPYETVAV
                     GTMNVTALPILHGGDEI"
     gene            complement(478..687)
                     /locus_tag="PLF67_09490"
     CDS             complement(478..687)
                     /locus_tag="PLF67_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90282.1"
                     /translation="MARKGFFSSLLSSLGVAVISFFILYFFFPNISNPYLGVAFKKPT
                     EIVDQESPSRLRQLFDQATSWLPFR"
     gene            complement(785..1399)
                     /locus_tag="PLF67_09495"
     CDS             complement(785..1399)
                     /locus_tag="PLF67_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90283.1"
                     /translation="MKKAWILLIVLAVLVPAAVSASPFGLSLGVTALYSDTFGQISTA
                     MEAGNYDGFANIDNYRFGADLRIKLLLAEIDAVAMFGSEAGATNISMLTTAGLSFDLF
                     NLVRIGAGVGPRFTVNIESDGTMWVKDSNGTKVDLENAGEAFLKSPLAYRLTADLKLG
                     KILVGLNYTLDTNYTFEEWQKFENIFNAPFDNGYMGVSVLFSFF"
     gene            complement(1454..2071)
                     /locus_tag="PLF67_09500"
     CDS             complement(1454..2071)
                     /locus_tag="PLF67_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90284.1"
                     /translation="MRKQVAVILTLLLFLSLPLTARSAFNLSLGFGATYCPPEKEDFS
                     SGMGDSRNWILSGELNGRLFFLQAQATVMLTKSEEDTQALKAIALVGLNLPVVGSWMV
                     MELSGGVGVTYVPQNPGVVKPYYALYGGKKAAVEEIPFLDAVLQSPIHLQMGIGTDFG
                     PLGLRARYVVESNQTIESVMAKGRWWEIFVPNSGCFSLAVLLKMS"
     gene            complement(2127..>2419)
                     /locus_tag="PLF67_09505"
     CDS             complement(2127..>2419)
                     /locus_tag="PLF67_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOE90285.1"
                     /translation="YELHDFFLYYTLRWGFGPKRIYRYAVKAFEESYTSSDIRHWLAV
                     FFTRFIANQFKRSASPDGPAVGSVSLSPRTGYRAPSDAQKQVWIEEIAGLMD"
BASE COUNT          631 a          679 c          592 g          517 t
ORIGIN      
        1 aaatctcatc accgccgtga agaatcggaa gggccgttac gttcatcgtc cccactgcaa
       61 cggtttcata cggctttatg acggtcagca gcagatgagg ccgtcctccg gcaacgacat
      121 ccgcgtcaag cgcataggaa aaacgaacac ggatcgtttc caccacgttc ctgctcgcat
      181 agacccgcag cggaccgtgt ctgctgaagg aacgcaaatc atcgattccg ttgatatgat
      241 cggcatggtc gtgggtataa aagaccgcat ccagacgagc aatcttgtgt ttcagcgctt
      301 gaagtcgaaa ctccggtccg gtatcgatga ccagcgcatg gttttcatct tcaatcaata
      361 cgcttgagcg cagacgcgta tcccgctcgt cgctcgaggt gcataccgga catgtacacc
      421 ctatgaccgg aatcccatga ctggttccgg tccctaaaaa agtcaatgag caagcggtca
      481 tcgaaacggc aaccaagagg tggcttgatc aaacaattga cgcagccgtg atggactttc
      541 ctgatcgacg atctccgtcg gcttcttaaa agcaactccc aaataaggat tactgatatt
      601 cggaaagaaa aaatagagga tgaagaatga aatcaccgcg acacccaaag aggaaagcag
      661 ggaagaaaaa aaaccttttc tggccatgtg gaactccttg aaccactata ccacacttgc
      721 gagtcctgtt gcaccgaaac aggcccgttt tcacgggcct gagcaaggag atgcgaacac
      781 tctgttagaa gaaggagaag aggacggaaa cgcccatata cccattgtcg aatggagcgt
      841 tgaagatgtt ctcgaacttc tgccactctt caaaggtata gttcgtatcc agcgtgtagt
      901 tcaatccgac aagaatcttt ccaagcttca gatcagcggt gaggcggtat gccaagggac
      961 tcttcaggaa cgcttcgccg gcattctcaa gatcgacctt ggtgccattg gagtctttta
     1021 cccacatcgt cccgtcggat tcgatgttga cggtgaagcg cggaccgaca ccggctccga
     1081 tccgaaccag gttgaacagg tcgaaggaga gaccggcggt ggtcaacatg gaaatgttcg
     1141 tcgcacctgc ctcggaaccg aacatcgcca ccgcgtcgat ctcggcgagc agaagcttga
     1201 tccgcaaatc tgctccaaag cgatagttgt cgatgttggc gaatccgtca tagttccccg
     1261 cttccattgc ggtggaaatc tgtccgaacg tgtcgctgta gagtgccgtc acaccgagac
     1321 tcaacccgaa tggcgatgcg ctcaccgccg ccggaacgag aaccgccaac acaatcaaaa
     1381 gaatccatgc ttttttcatt ctgtcacctc tttctgctac acactgtcta tttataagct
     1441 tcgtgtgtaa aatttaactc atcttcaaaa ggacagcaag ggaaaaacat ccgctattcg
     1501 gaacaaatat ctcccaccac ctgccttttg ccattaccga ttcaatcgtc tgattcgatt
     1561 cgaccacgta ccgggccctc agtcccaacg gtccgaagtc ggtcccgatt cccatctgca
     1621 gatggatcgg actctgaagc accgcatcaa ggaacggaat ctcctcgacc gccgccttct
     1681 ttccgccgta cagcgcatag tagggtttca cgaccccggg gttctgcggg acgtacgtca
     1741 cacccactcc cccactgagc tccatcacca tccacgagcc gaccaccgga agattcaacc
     1801 ccacaagggc gatcgccttg agcgcctgcg tgtcctcttc actcttcgta agcatcaccg
     1861 tcgcttgagc ctggagaaaa aaaagccgtc cgttcaactc accgctgaga atccaattcc
     1921 gggaatctcc cattccggaa gaaaaatcct ccttttccgg ggggcagtac gtcgcaccaa
     1981 agccaaggct gaggttgaac gcacttcgcg cggtcaacgg cagagacaaa aaaagcagaa
     2041 gggtcaagat gaccgccact tgtttacgca tgactgattg ttgctcccgt actcatataa
     2101 caacgatatg agtaaaaggg tacaaattaa tccatcaatc ctgcaatttc ttcaatccaa
     2161 acctgttttt gggcatcgct gggcgcccga taccctgttc gaggggacag ggagaccgat
     2221 ccgaccgccg gtccgtcggg tgaagcggag cgcttgaatt gattggcgat gaagcgggtg
     2281 aagaacacgg cgagccaatg tcgtatatcc gatgaggtgt acgattcctc gaatgctttg
     2341 accgcatacc ggtagatcct cttcggaccg aacccccagc gcagggtgta gtaaaggaag
     2401 aaatcatgga gctcatacg
//
LOCUS       DAOSLI010000125         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_37816_length_2417_cov_14.817104, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000125 DAOSLI010000000
VERSION     DAOSLI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37816_length_2417_cov_14.817104"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1879)
                     /locus_tag="PLF67_09510"
     CDS             complement(152..1879)
                     /locus_tag="PLF67_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE90286.1"
                     /translation="MNVVQDAIVEIDFKKQQLIGQQAKYGYDELIIGTGATTADFNIP
                     GVKEHAFYLWSLEEAIKIRCHIEETVRLAALEADEKKRKQMLTFVVAGGGFTGVEMVG
                     ELLEYLPILCKTNKIDYEKEVQLILCEGLGTILNMLPEKPMKKAWEYMKKKGVKIMVN
                     NLITNVTADGFTTRQGADIKTKTLIWTCGIRGTEFGEKLKITDGKIGRKLVNEYMQTP
                     DYENVYVVGDGMWFLENNRAVPQIVEAAEQTAKTAARSIIYKVKTELGLKAKEPVPFK
                     SNFHGFMVSIGGRYAVSHTAGISLSGFFAMALKHLVNVYYQSGVCGINGAWAYLKHEI
                     FNIKNNRSLIGGLAANKVPAYWITFLRMFLGVMWLIEGGGKIADGWLTDTTGSKVYWG
                     SADGVAGATGAVTEAVASATTAATQAVASATQAVADTVSGATGEVAQFAPPLLKEPLA
                     IYTWINETFVAQAPYFFQVVIVLVEVAIGLMFIGGFLTFPAAIVSLGLSIMFLIGALA
                     GKEILWYMAVSIVMLGGAGKAFGLDYWVMPWLKKIWNMTPLAKKTYLYLDEPTFSRKQ
                     MERRLNKKK"
     gene            complement(1876..2103)
                     /locus_tag="PLF67_09515"
     CDS             complement(1876..2103)
                     /locus_tag="PLF67_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE90287.1"
                     /translation="MNKKKIVVLGGGYAGVHAAKTLNKAFKKHQDKVEVTLIDKKHHH
                     ILLTEPHEVAGARVSEDSARYRLTESLPGKK"
BASE COUNT          554 a          647 c          593 g          623 t
ORIGIN      
        1 gcaggacgca acatcttgcc gccggttcga acgtagtcca acagcacttg gcgaataaag
       61 ggatgggaag ctgcgatcgt ctcttcaatc gtagccttca cccggttcag cggttcggcc
      121 aatgcttgtt cttcttccca gaattccgcc attacttttt cttgttgagt ctgcgctcca
      181 tctgcttgcg tgagaaggtc ggctcatcaa ggtagaggta ggtcttcttg gccagaggag
      241 tcatattcca aatcttcttc agccaaggca tcacccagta atcaaggccg aacgccttgc
      301 cggctccacc gagcatgacg attgaaactg ccatatacca gagaatttct tttccggcca
      361 acgctcctat gaggaacatg atggagaggc caagggagac gatcgctgca gggaacgtca
      421 ggaagcctcc gatgaacatt aatccgatcg ccacttcgac gagaacgatt acgacttgga
      481 agaagtaagg cgcctgagcg acaaacgtct cgttgatcca agtatagatt gccagcggtt
      541 ccttcaagag gggaggagcg aattgggcaa cctcgccggt agcgccggaa acggtatccg
      601 caactgcctg agtcgccgat gcaaccgctt gcgttgcagc ggttgttgcc gatgcaacag
      661 cctcggtaac ggcaccggtt gcacctgcaa cgccatccgc cgaaccccaa tagactttgg
      721 atccggtcgt atcggtcagc cagccgtcgg cgatctttcc gccgccttcg atcagccaca
      781 tcacaccgag gaacattctg aggaacgtga tccagtacgc cgggaccttg tttgccgcca
      841 atcctccgat gagggaacgg ttgttcttga tgttgaagat ctcatgcttc aggtatgccc
      901 acgcaccgtt gataccgcac actccgcttt ggtagtagac gttaaccaag tgtttaagcg
      961 ccattgcaaa gaagccggaa aggctgatcc ctgcagtatg gcttaccgca tagcgtccgc
     1021 cgatggaaac catgaagccg tgaaagttcg acttgaacgg aaccggttct ttcgccttca
     1081 gaccgagttc cgtttttacc ttgtagatga tcgaccgggc agcggtcttc gccgtctgct
     1141 cggcggcctc aacgatctgg ggaacggcgc ggttgttctc aaggaaccac attccgtccc
     1201 caacgacgta gacattctca tagtcggggg tctgcatata ttcgttgacg agcttgcggc
     1261 cgatctttcc gtcggtgatc ttgagctttt cgccaaactc ggttcccctg attccgcagg
     1321 tccagatgag ggtcttggtc ttgatgtcgg caccttggcg ggtggtgaat ccatctgcgg
     1381 tgacgttcgt gatcaggttg ttgaccatga tcttcacacc cttctttttc atatactccc
     1441 aagctttctt catcggcttt tcggggagca tgttgaggat cgtgcccaag ccttcgcaaa
     1501 gaatcagctg aacttctttt tcgtagtcga tcttgttcgt tttgcagagg atcggcaggt
     1561 actccaaaag ttcacccacc atctcgacgc cggtgaagcc gccgccggca acgacgaagg
     1621 taagcatctg cttgcgcttc ttctcatccg cttcaagggc ggccaatcga accgtctctt
     1681 caatgtgaca gcggatcttg atcgcttctt ccaaggacca gagatagaaa gcgtgttctt
     1741 ttacccccgg aatattgaaa tcggcagtgg tggcaccggt gccgatgatc agttcatcgt
     1801 aaccatattt tgcctgctgg ccaataagct gctgcttttt aaaatcgatt tcgacgatcg
     1861 cgtcctgaac cacgttcact tttttcccgg caaagattcg gtcaaacgat atcttgctga
     1921 atcttcgctt acccgggccc ctgcgacctc gtgcggctca gtaagcagga tgtggtgatg
     1981 cttcttgtca atgagcgtca cctcgacttt gtcctgatgc tttttaaacg ctttgttcag
     2041 tgtcttggct gcgtggactc cggcatagcc tccacccaag accacaatct tcttcttgtt
     2101 catagtacta ccttccttaa gaaaacaggg tttcaaccct gccaaatgta gttgaccaat
     2161 atcctcgata gacgtggtaa ctctaccaca agtgcaggtg ctagtacaat tctttgtttt
     2221 tatatcgata cgacggtttc aattgagagg agctcaacac accgtccgaa acggaagaaa
     2281 tgccttgatt tttcaacaaa aagagcatct tcaatgtttt caggaatccg gattgaaatc
     2341 aaacgtgaaa tcgataaaga acaatttata tggttgatgc caatcgtctt tccaacataa
     2401 catatttata gatagag
//
LOCUS       DAOSLI010000126         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_37985_length_2410_cov_30.433121, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000126 DAOSLI010000000
VERSION     DAOSLI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37985_length_2410_cov_30.433121"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLF67_09520"
     CDS             <1..222
                     /locus_tag="PLF67_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90288.1"
                     /translation="LVKGRIVFTEDTFHFFTKEKGRVVEDLAIPLDRIMSLSTDSIVE
                     SKKALVIVTGDASYAFTLSKAIDGESCSV"
     gene            287..826
                     /locus_tag="PLF67_09525"
     CDS             287..826
                     /locus_tag="PLF67_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90289.1"
                     /translation="MKHVTAVLLLMFIGTHVVFGSTNVELKTDCASLISLFGETISAR
                     IEARLDLSDEFALMLPVELTAEKGRGQPIYLESGLFLAYHPFSKAAAIYASLIQLGIL
                     FERRYGETETFFLNEVGFGYTFRLPFGLLIEPRMTIKDPNSVFTSDYQELKEAFSSYP
                     MIRFALYGGWSFTLPSATT"
     gene            839..1684
                     /locus_tag="PLF67_09530"
     CDS             839..1684
                     /locus_tag="PLF67_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90290.1"
                     /translation="MKRWMNTIVAILLIIPISGCTMGLEMKKKHAVTEEQVLSGVSVI
                     LDAQKETVWPYLAEEMDVLSRGGIPSAEEIIRNTLAEELGMEYLEFCYVVGTGETQED
                     LDLVTEYAKTLLDEEGKAELERSLVQVREILLAEAGDLARRLAPSQRVPFWKDMQKLV
                     TRTMVLFTAGIVYACMPTTVFWGKVSAAAAIAVAAGIVSETVMAIWRYYQFGGDPDEA
                     FGEWLKGVTTEPELSYALTASVMAVGKTMKRGPVVTGILICVFAIYNVIDIVKPMLKR
                     YNFNV"
     gene            1759..>2410
                     /gene="pepT"
                     /locus_tag="PLF67_09535"
     CDS             1759..>2410
                     /gene="pepT"
                     /locus_tag="PLF67_09535"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripeptide aminopeptidase PepT"
                     /protein_id="HOE90291.1"
                     /translation="MITRLKEDILKRFLRYVQVDTMSDDRVVDTKRPSTDGQWDLLKM
                     LASELVELGVDDVHIDEKGIVIAKIPSNMKKAVPVVGFMAHVDTADDVPGNGVKPRVI
                     EKYDGGDIPLNEEHSIIAVENPELSNYIGETLIVTDGTTLLGSDDKAGIAEIMSAVRH
                     LMEHPEIEHGGINIIFTSDEETGAGMDGFPYEKLDCEYCYTVDGGRRFEIQSECFNA"
BASE COUNT          532 a          641 c          739 g          498 t
ORIGIN      
        1 cttgtcaaag gacggatcgt cttcaccgag gacaccttcc atttcttcac caaggagaag
       61 gggcgggtgg tggaggatct tgccatcccc ctcgaccgga tcatgagcct ctcgacggat
      121 tcgatcgtcg aatcgaaaaa agctctcgtg atcgtcaccg gggacgcctc ctacgccttt
      181 acgctttcca aggcgatcga cggggagagc tgctctgtct gagctacctc aaacggggaa
      241 tatgatggac cgataggctg tggtaccttc ctcgcaaggg gaagagatga aacacgtcac
      301 agcggtattg ctcctcatgt ttatcggaac acatgttgtc ttcggctcca cgaatgttga
      361 gttgaagacc gattgcgcct cgttgatctc gctcttcggc gagacgattt cggctcgcat
      421 tgaagcccgg ctcgatctga gtgacgagtt cgctctgatg ctgcccgttg aactgacggc
      481 ggaaaagggc agggggcaac cgatctatct ggagagcggc ctgtttctcg cctaccaccc
      541 gttttccaag gcggcggcaa tctatgcatc tctcatccag ttgggaatcc tttttgaacg
      601 gcggtacggg gaaacggaga cattctttct gaacgaggtg ggcttcggct acaccttccg
      661 tcttcccttc ggactgctca tcgagccgcg gatgaccata aaagatccca acagtgtttt
      721 cacaagcgat tatcaggaat tgaaggaggc cttcagttcc tatccgatga tacggttcgc
      781 tctctacggc gggtggtcct tcaccctgcc gtcggcaaca acctaagagg aggaagtgat
      841 gaagcgatgg atgaatacga tcgtggcaat cctgttgatc atccccattt cggggtgcac
      901 gatggggttg gagatgaaga agaaacacgc tgtgacggag gagcaggtcc tcagcggggt
      961 ctcggtaatc cttgacgccc agaaagagac cgtctggccg tacctggccg aggagatgga
     1021 tgtcctgagt cgagggggca ttccgtcggc ggaggagatc attcgaaaca cccttgccga
     1081 agagctgggg atggagtatc tggagttctg ctacgtcgtc ggcaccgggg agactcagga
     1141 ggacctggat ctggtgacgg agtacgcgaa gacgctcctg gacgaggagg gaaaggcgga
     1201 gctggaacgt tcgcttgtcc aagtcaggga gatcctgctt gccgaggcgg gggatctggc
     1261 caggcgcctt gcacccagtc aacgggtgcc gttctggaag gacatgcaga agctcgtgac
     1321 ccggacgatg gtcctcttca ccgccggaat cgtgtatgcg tgcatgccga cgacggtctt
     1381 ctgggggaag gtgtccgccg ccgcggcgat cgccgtggca gcggggatcg tcagcgaaac
     1441 ggtaatggcg atctggcggt actaccagtt cggcggcgat cccgatgaag cgttcggcga
     1501 gtggctcaaa ggggttacga ccgagcccga gctctcctat gcgttgaccg ccagcgtgat
     1561 ggcggtgggc aaaaccatga agcggggacc ggtcgtgacg ggaatcctca tctgtgtttt
     1621 tgcgatctac aacgtcatcg acatcgtgaa accgatgctg aaacggtaca atttcaacgt
     1681 ctaggccgtc tcgcttcctc ctcaatgttg atgaggtccg cactttgtgt gatactgaat
     1741 caatctgagg aggaagatat gatcacccgg ttgaaagaag atattctcaa gcggtttctc
     1801 cgctacgtcc aagtcgacac gatgagcgac gatcgcgtcg tcgacaccaa gcgcccctct
     1861 accgacgggc agtgggatct cttgaagatg ctcgcctccg agcttgtcga acttggagtc
     1921 gatgatgtgc atatcgatga gaaggggatc gtcattgcga agattccctc caacatgaag
     1981 aaagcggtgc cggtcgtcgg cttcatggcg cacgtcgata cagccgatga tgtgccgggc
     2041 aacggggtaa agccccgggt gatcgagaag tatgacggag gggacatccc cctcaacgag
     2101 gaacactcga tcatcgcggt tgaaaatccc gagctatcca actacatcgg agagacgctc
     2161 atcgtcaccg acgggacgac cctgctcggc agcgacgaca aggccggaat cgccgagatc
     2221 atgagcgcgg ttcgccatct tatggaacac cccgagattg aacacggcgg aatcaacatc
     2281 atcttcacca gcgacgagga gaccggtgcc gggatggacg ggttccccta cgagaagctc
     2341 gactgtgagt actgctacac ggtcgacgga ggcaggcgct tcgagatcca gagcgaatgc
     2401 ttcaacgccg
//
LOCUS       DAOSLI010000127         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_37986_length_2410_cov_26.481104, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000127 DAOSLI010000000
VERSION     DAOSLI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37986_length_2410_cov_26.481104"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /gene="pgmB"
                     /locus_tag="PLF67_09540"
     CDS             <1..1407
                     /gene="pgmB"
                     /locus_tag="PLF67_09540"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOE90292.1"
                     /translation="YTNMMVRHHLRRLKEFIDESSLTKEELNTLDKAIETIVIPFDKE
                     KGITLQDDSFLSKEIWDETKKGPLKRPMLLHYHPLVIYRHRLIKQADTVLAHVLLSDE
                     TPWYQKRRDMLFYEPLTTGDSSLSAGIQGILAFEIGEIPLAVTYLEDTTLTDIRDLHS
                     NTKDGLHTAAMAASWMGIVYGVAGYRYLKNTPTFRPRLPDGRAGVGFSLTFGDVTLTI
                     RIDKEETTYQATGPITIAHRSTKLNVTDTPITIKTKPTCKAVIFDLDGVITSTDDDHY
                     RAWKAIADKSGLAFDRTINQELRGVSRTESLKIILKHNKRTLADDEIKKLCDEKNEIY
                     RSFLTELTPKDILPNIEALLKQLKEKNMKIGLASASQNARTILSNLKLTHYFDVIVDA
                     KDLIKPKPDPEIFARAADALGFYPEECTGVEDAKAGIEAINAAMMKAVGIGDAVDPAE
                     CDVHRKDTRMLTIEDLLF"
     gene            complement(1404..2363)
                     /locus_tag="PLF67_09545"
     CDS             complement(1404..2363)
                     /locus_tag="PLF67_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE90293.1"
                     /translation="MGKITIEDIAREAGVGIGTVSRVLNNSVHVAEDTRKRVLDVIKA
                     RQYVRSAAASKLARNNVVETTVGLLLPDIGNHYFFEIFEAIYQKFRGLGIDLIIFNYE
                     KHNPKVIQRILDAQLSALLIFAFHLVDSERDMLLRHNVPYLYVDYPTRTEHSICVDNT
                     QGGRIAAQYLHGKGVRKPCYIAVDPPAWSNEQRYAGFTGEYLQYGVSSIPVFSAFLSE
                     EDGYRAAEEIIALGTHDGIFCYCDDIAAGAIRAIRKHDAPIRVIGFDGVRATDYLELS
                     TVSQEPQMIGELASTLIIDLIEGKGSGGLIHRVITPVLIDRGS"
BASE COUNT          559 a          659 c          700 g          492 t
ORIGIN      
        1 tacaccaaca tgatggtccg ccatcatttg aggaggctca aggagttcat cgacgaatcc
       61 tcgctcacaa aagaggaact gaacacgctt gacaaagcga tcgaaacgat cgtcatcccc
      121 ttcgacaaag aaaaaggaat caccctgcag gatgattcct tcctttccaa agagatctgg
      181 gatgaaacga aaaaaggtcc gctgaagcgt ccgatgctgc ttcactacca tccgctggtc
      241 atctaccgcc accgcctgat caagcaggcg gacacggtgc tcgcccatgt gctgttaagc
      301 gatgagacgc cttggtatca gaagcggaga gacatgctgt tctatgagcc gctgaccacg
      361 ggagactcct ccctgtctgc ggggatccag gggatcttgg cgttcgagat cggggagatt
      421 cctcttgccg tcacctatct ggaagacacc accctgaccg acatccggga ccttcactcc
      481 aacacgaagg acggcctgca taccgccgcg atggcggctt cgtggatggg catcgtctat
      541 ggtgtggcgg gctacagata cctcaagaac acccccacct tccggccccg gctacccgac
      601 ggccgggccg gggtggggtt ctccctcacc ttcggtgatg taacgcttac catacgcatt
      661 gacaaagagg agacgaccta tcaggcgacc gggccgatta cgatcgccca caggtcgacg
      721 aagctcaacg taacagacac accgataacg atcaaaacaa aaccgacctg caaagcggtg
      781 atcttcgacc ttgacggggt gatcacctcc accgatgatg atcattaccg ggcctggaag
      841 gcgatcgccg acaaaagcgg tcttgccttc gatcggacga tcaaccagga gctgagaggg
      901 gtgtcccgaa cggagtccct gaagatcatc ctgaaacaca acaagagaac gcttgcggat
      961 gatgaaataa aaaagctctg cgatgaaaag aacgagattt atcgttcctt cctcacagag
     1021 cttactccga aggatattct gcccaatatc gaggcgcttc tcaagcagtt gaaagagaag
     1081 aacatgaaga tcggtctcgc ttcggcgtcc caaaacgccc ggacgatcct gtccaacctc
     1141 aagctgactc actatttcga tgtgatcgtg gatgcgaagg atctgatcaa gcccaagccg
     1201 gatcccgaga tcttcgcccg ggcggccgat gcgcttggat tctatccgga agaatgcacc
     1261 ggggtcgagg atgccaaggc ggggatcgag gcgatcaatg cagcgatgat gaaggcggtc
     1321 ggaatcgggg atgcggtgga tcccgccgaa tgtgatgtcc acagaaaaga tacaagaatg
     1381 ctgacgatcg aggatctgct cttttagctg cctcgatcaa ttaataccgg ggtgatgacc
     1441 ctgtggatca gaccgcctga tcctttgcct tcgatgaggt cgatgatcag ggtcgaggcc
     1501 agctctccga tcatctgggg ttcctggctg acggtggaga gttccaggta gtcggtcgcc
     1561 cggactccgt cgaagccgat gaccctgatg ggggcgtcgt gcttgcggat cgcccggatc
     1621 gctccggcgg cgatgtcgtc gcagtagcag aagatgccgt catgcgttcc cagggcgatg
     1681 atctcttccg ctgcccgata cccgtcttct tccgacagga aagcgctgaa gacggggatc
     1741 gaggagactc cgtattgtag gtactcgccg gtaaagccgg catagcgctg ttcgttcgac
     1801 caggcgggtg gatcgacagc gatgtaacac ggcttccgaa cacctttgcc atgcaggtat
     1861 tgggcggcga ttcttccgcc ctgggtgttg tcgacgcaga tgctgtgctc ggtccgggtg
     1921 ggataatcga cgtagaggta cgggacgttg tggcgaagga gcatgtcccg ttcactgtcg
     1981 acgaggtgaa aggcgaagat cagcaacgcc gagagctgcg catcaaggat gcgctggatg
     2041 actttggggt tgtgtttttc gtagttgaag atgatgaggt cgattccgag tccccggaac
     2101 ttctggtaga tcgcttcgaa gatctcgaag aagtagtggt ttccgatgtc aggcagcagg
     2161 aggcccaccg tcgtctccac gacgttgttt ctcgccagct tggaggcggc ggcgcttcga
     2221 acatactgtc gggccttgat gacgtcaaga acacgcttgc gcgtatcctc ggccacatga
     2281 acgctgttgt tgagtacccg ggacacggtc ccgatcccca ccccagcctc ccgagcgata
     2341 tcttcaatgg ttatcttacc catgccaaaa cgatagagtg tttaccaaca tgagtcaacg
     2401 gaggggggga
//
LOCUS       DAOSLI010000128         2389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_38389_length_2389_cov_28.767352, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000128 DAOSLI010000000
VERSION     DAOSLI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38389_length_2389_cov_28.767352"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLF67_09550"
     CDS             complement(<1..268)
                     /locus_tag="PLF67_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90294.1"
                     /translation="MLKILMLLLVGGISILNRFYIGVGFYYLMILLLAAVALLYWRLA
                     FHLSVKETGITDPIRLTCSSSKITEDELVKGRIVFTEDTFHFFTK"
     gene            367..>2389
                     /locus_tag="PLF67_09555"
     CDS             367..>2389
                     /locus_tag="PLF67_09555"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HOE90295.1"
                     /translation="MKRHKHFFTLFVLVITLVIAGTSCTDPGPEVGTLTLNMQMSSRS
                     RALIPGETPLEVARYIVMGTGPQGATFNVSTSTKSVDIEGLLLGEWSIRAIGQNQGGT
                     DLVEGTIDTIIGTSPNIVIIELNELIGKGTLNLTLDWSEIEIASPSTTLRLIDAEGNT
                     RTLNPTVNNAANGSVTYQELLDAGSYTVTAQLENNGEPIAGFADFIRIVSNRTTEAQI
                     TFEIEDYTTLPASVTMVDHLGTPIECEIAGLPEEVPAFEPVTVYLSSDSDAVIDAVWY
                     LDGAEIARGMECTFRTAIGSHRLDVLAQGALLASLGSATISFESKVIGTSGVPVLLRN
                     VSSSSDGITIDAGCVTKLLPNNILIVGNNTEQAIHVCRIVRGSLEVLHTYTRAMSGYH
                     TKKLTDIYYDPLSSQVIISDEDGPRVSLYLYNPSTHELTLRLVKDNIYYSKGSTVLRF
                     NKLSNFKLDKLNGYLYGLIGGKNEITAVLINSATAEELKQNVGQAFHSSGYEFSDWDT
                     SPSSNHTVLVSPQYNCVLIASRSAATGTMVQNMKLYLQDTPYVTGARSVCFIGEETFV
                     YSTANNLVRCRYTAQGDAMTQTDVYSKGDGHGTARGITQMVASPSMRYLYTLSPSERA
                     LDVYEISEGTEELFHIETIPMGSFNGTDMEISPDGTHLVVTSETAQSLLL"
BASE COUNT          572 a          717 c          656 g          444 t
ORIGIN      
        1 ccttggtgaa gaaatggaag gtgtcctcgg tgaagacgat ccgtcctttg acaagttcat
       61 cctcggtgat ctttgaagat gaacaggtaa gccggatcgg atcggtgatc cccgtctctt
      121 tcaccgaaag atggaacgcc aggcgccagt acagcaaggc taccgcggcg agcagaagga
      181 tcatcaagta atagaagccg actccgatat agaagcggtt gaggatcgag attcctccaa
      241 cgagcaacaa cataagaatt ttcagcacgg tcaacatctg aataagcaat ctacctcagt
      301 cggggcaaaa aagaaagagc gggaagcgat atcccgactc tccccccttc cagggtggag
      361 gatatcatga agcgacacaa acattttttc acgcttttcg ttttggtcat cacgcttgtc
      421 atagcgggaa cctcgtgcac ggatccggga ccggaggtcg gaacgctcac cctcaatatg
      481 cagatgagct cccgttcccg agcactgata ccgggggaaa caccactgga agtggcccgc
      541 tacatcgtga tgggaaccgg ccctcagggg gctacgttca atgtttcaac ctcgaccaag
      601 agtgttgata tcgaagggtt gctgcttggc gaatggagca tccgggcgat cgggcagaac
      661 caggggggca ccgatctggt cgagggaacg atcgatacga tcatcggcac ctctccgaac
      721 atcgtcatca tcgaattgaa tgaactgatc ggaaaaggaa ccctcaacct caccctcgac
      781 tggagcgaga tcgagatcgc ttccccgtcg acgacgctca ggctcatcga tgccgaaggg
      841 aacacccgaa cgctcaatcc gacggtgaac aatgcggcga acggctcggt cacctaccag
      901 gagctgctgg atgccggttc ctacaccgtg acggcgcagc tggaaaacaa cggagagccg
      961 atcgccggat ttgccgactt catccggatc gtcagcaatc gaaccaccga agcccagatc
     1021 acctttgaga tcgaggacta cacgacgctt cccgcttccg tgacgatggt cgatcatctg
     1081 ggcaccccga tcgagtgcga aatcgccggg ctgcccgagg aggtccccgc gttcgagccg
     1141 gtcaccgtgt acctcagctc cgacagtgat gcggtcatcg atgcggtgtg gtatctggac
     1201 ggcgcggaga tcgcccgcgg aatggagtgc accttcagga cggcgatcgg atcgcaccga
     1261 ttggacgtgc ttgcccaagg cgctctcctc gcctccctgg gatcggcgac gatctccttt
     1321 gaatcgaaag tcatcggcac ctcgggcgtt ccggtgctcc tgcgcaacgt ctcttcttcc
     1381 agcgacggaa tcacgatcga tgcggggtgc gtcaccaaac tcctgcccaa caacatcctc
     1441 atcgtgggga acaacaccga gcaggcgatc catgtgtgcc gaatcgtccg cggctccctt
     1501 gaagtgcttc acacgtacac gagggcgatg agcgggtatc acacgaagaa actgaccgac
     1561 atctactacg atccgctcag cagtcaggtg atcatcagcg acgaggacgg accgagggtc
     1621 agcctctatc tctacaaccc ctcaacccac gagctcaccc ttcggcttgt gaaagacaac
     1681 atctactact ccaagggcag tacggttctc cggttcaaca agctctccaa cttcaagctg
     1741 gacaagctga acggatatct gtacggcctc atcggtggaa aaaacgagat caccgccgtc
     1801 ctgatcaact cggcaaccgc cgaagagctg aagcagaacg tcggtcaggc gttccactcg
     1861 agcgggtatg agttcagcga ctgggacacc agtccgagtt ccaatcacac cgtactggtg
     1921 agcccgcagt acaactgcgt cctgatcgcc agccgatcgg cggccaccgg cacgatggtt
     1981 caaaacatga agctctatct gcaggacacg ccgtatgtca ccggggctcg gagcgtctgc
     2041 ttcatcggcg aggagacgtt tgtctactcc accgccaaca acctcgtccg ctgcagatac
     2101 acagcccaag gcgatgcgat gactcagacg gatgtgtaca gcaaaggcga cggacacgga
     2161 acagctcggg gaatcacgca gatggtggcc tccccctcga tgcggtacct ctacaccctc
     2221 agtccaagcg aacgggctct ggatgtgtat gagatcagcg aaggcaccga ggaactcttc
     2281 cacatcgaga cgatcccgat gggctcgttc aacggaaccg acatggagat ctcccccgac
     2341 ggaacccacc tggtcgtcac ttcggagacc gctcaatcgc ttctgctct
//
LOCUS       DAOSLI010000129         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_39105_length_2356_cov_21.839635, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000129 DAOSLI010000000
VERSION     DAOSLI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39105_length_2356_cov_21.839635"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..538
                     /gene="fucU"
                     /locus_tag="PLF67_09560"
     CDS             113..538
                     /gene="fucU"
                     /locus_tag="PLF67_09560"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HOE90296.1"
                     /translation="MLKTIPSILSPELLKVLMEMGHGDELVIGDGNFPAASMNDRVVR
                     LDGHGGEEILKAILQLFPLDTYAPNAFLMEKVPGDPAQTPIWDSYEKIAKAGDPGFTG
                     FTFVERFAFYEQSKKAYAIVATGESALYANIILKKGVVL"
     gene            complement(535..1422)
                     /locus_tag="PLF67_09565"
     CDS             complement(535..1422)
                     /locus_tag="PLF67_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE90297.1"
                     /translation="MSLVLRASDYFYDPSFPIKVLERDPEIPYPLHSHDFWELIVIVS
                     GSGTHFDTAGSFELTSGNVFVIPPGFKHGYRDVRNLRLFNILFDVSMFEESFFDLSNM
                     SGYQALLKVEPSYRHDGSLSTLMQLTPAQQARVLPLLEHMLIESDSTESEIGAKAGAF
                     AILAQLLVMLFRFYAEQPRKDNQTVMRIADALSFLEAHPDRSVSTSELMELTNMSAST
                     LNRHFKNATGLSPIEFHIQRRIKHACRLLRSSDMSVSEISEATGFEDSNYFSRQFRNV
                     MGITPLSYRKNRSMWYRLP"
     gene            1507..>2356
                     /locus_tag="PLF67_09570"
     CDS             1507..>2356
                     /locus_tag="PLF67_09570"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOE90298.1"
                     /translation="METTSYQYAKERYRTIGVDTDAVLKRLDRIPISIHCWQGDDVGG
                     FETPDAELSGGGIQVTGSYPGKAQTIEQLRADLEEVLSIVPGPHRISLHASYGEFGSE
                     FVDRNEIEPNHFRGWVDWAQANNVHLDFNGTFFSHPKANDGYTLASKDEEIRQFWIEH
                     AKRTRKIAAWIGKQQGSPCILNTWIPDGAKNHTADKMGYRAILKESLDEIFRIEYPRE
                     HMRDALETKLFGIGSEAFVVGSHEFYMNYAARNDVMLCLDMGHFHTEEDVSDKLSSIL
                     LFDDELL"
BASE COUNT          554 a          613 c          696 g          493 t
ORIGIN      
        1 atgtttgcca tctcgttctc cttggtgtat gatggttttg taatcgttta cataccttca
       61 gtattacaca gcatccaagc gattgcaacg aaaaaaaaag gagtaacgtg ccatgcttaa
      121 aacaattccc tcgattctca gtcccgagct gctaaaggtt cttatggaga tggggcacgg
      181 cgatgaactg gtcatcggcg acggaaactt ccccgccgca tcaatgaacg accgggtcgt
      241 ccgcctcgac ggtcacggcg gtgaggagat tctgaaagcg atcctgcagc ttttccctct
      301 agatacctac gcccccaacg ccttcctcat ggagaaggtt cccggcgatc ctgcccaaac
      361 cccgatctgg gattcctatg aaaaaatcgc caaagcgggt gatcccggct tcaccgggtt
      421 caccttcgtc gagcgctttg cgttctatga gcagtcgaag aaggcgtatg cgatcgtggc
      481 gaccggcgaa agcgccctgt acgcgaacat catcctcaag aaaggcgtcg ttctctaagg
      541 gagccgatac cacatcgagc ggttcttccg ataggagagc ggcgtgattc ccatcacgtt
      601 gcgaaactgc cggctgaagt agttggagtc ctcgaacccc gtagcttcgc tgatctcgct
      661 gaccgacatg tcgcttgagc ggagaagccg gcacgcatgc ttgatcctcc gttggatgtg
      721 gaattcgatg ggggagaggc cggtcgcgtt cttgaagtgc cggttgaggg tgcttgccga
      781 catgttggtc agttccatca attcgctcgt tgaaacggag cggtcgggat gggcttccaa
      841 aaagctgagg gcgtcggcga tccgcatgac ggtctggttg tccttccgcg gctgctcggc
      901 ataaaagcga aacagcatca cgaggagctg cgcgaggatc gcgaacgctc cggctttcgc
      961 cccgatctcg ctttccgtcg aatccgattc gatgagcatg tgctcaagca ggggaagtac
     1021 ccgcgcctgc tgggcggggg tgagctgcat cagggtcgac agcgaaccgt catggcggta
     1081 ggagggctcc accttcaaaa gcgcctgata gccgctcatg ttgctcagat cgaagaacga
     1141 ttcttcgaac attgagacgt cgaagaggat gttgaagagg cgcaggttgc ggacatcccg
     1201 atagccgtgt ttaaagccgg gagggatgac gaagacgttt ccgctggtca gctcgaacga
     1261 gccggcggtg tcgaagtggg ttccgcttcc cgagacgatg acgatcagtt cccagaagtc
     1321 gtggctgtgc agaggatagg ggatctcggg atcccgttca agaaccttga tggggaagga
     1381 agggtcgtaa aaatagtcgg aagcccggag tactaagctc attatgacaa aatagtgcta
     1441 aaatatgcct attatgtcaa gacaaaaatg gtaaagtggg ggtaacatta tagataggag
     1501 gagtgtatgg aaaccacttc atatcagtac gcgaaagaac ggtaccgaac gatcggcgtt
     1561 gataccgatg cagtcctcaa gcggcttgat cggattccga tctcgattca ttgctggcag
     1621 ggcgatgatg tcggcgggtt tgaaactccc gatgccgagc tcagcggcgg tggaattcag
     1681 gtcaccggct cctatcccgg caaagcgcag acgatcgagc agctgagagc cgatcttgaa
     1741 gaggtgcttt cgatcgtccc tggtccgcat aggatcagcc tccatgcgag ctacggcgag
     1801 ttcggctccg agttcgtcga ccgaaacgag attgaaccga accacttcag gggatgggtg
     1861 gattgggcgc aagcgaacaa cgtccacctt gatttcaacg gaaccttttt cagccacccg
     1921 aaagcgaatg acggatatac cctggcgagc aaggatgagg agatccgcca gttctggatc
     1981 gagcacgcca agcgaacccg gaagatcgca gcgtggatcg gaaagcagca gggatcgccg
     2041 tgcatcctca acacgtggat tcccgacggg gcgaaaaacc acaccgccga caagatgggg
     2101 tatcgggcga tcctcaagga gagcctcgat gagatcttca ggatcgaata cccccgcgag
     2161 catatgcggg acgctcttga aacgaagctt ttcgggatcg gcagcgaagc gttcgtcgtc
     2221 ggctcacacg agttctacat gaactatgcc gcccggaacg atgtgatgct ctgtctcgac
     2281 atggggcact tccacaccga agaggatgtc tccgacaagc tcagttcgat cctcctgttt
     2341 gatgatgaac tgctga
//
LOCUS       DAOSLI010000130         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_39126_length_2355_cov_19.755652, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000130 DAOSLI010000000
VERSION     DAOSLI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39126_length_2355_cov_19.755652"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLF67_09575"
     CDS             complement(<1..850)
                     /locus_tag="PLF67_09575"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOE90299.1"
                     /translation="METTSYQYAKERYRTIGVDTDAVLKRLDRIPISIHCWQGDDVGG
                     FETPDAELSGGGIQVTGSYPGKAQTIEQLRADLEEVLSIVPGPHRISLHASYGEFGSE
                     FVDRNEIEPKHFQGWVDWAQANNVHLDFNGTFFSHPMANDGYTLASKDEEIRQFWIEH
                     AKRTRKIAAWIGKQQGSPCILNTWIPDGAKNHTADKMGYRAILKESLDEIFTTEYPRE
                     HMRDALETKLFGIGSEAFVVGSHEFYMNYAARNGVMLCLDMGHFHTEEDVSDKLSSIL
                     LFDDELL"
     gene            935..1822
                     /locus_tag="PLF67_09580"
     CDS             935..1822
                     /locus_tag="PLF67_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE90300.1"
                     /translation="MSLVLRASDYFYDPSFPIKVLERDPEIPYPLHSHDFWELIVIVS
                     GSGTHFDTAGSFELTSGNVFVIPPGFKHGYRDVRDLRLFNILFDISMFEESFFDLSNM
                     SGYQALLKVEPSYRHDGSLSTLMQLTPAQQARVLPLLEHMLVESDSTESEIGAKAGAF
                     AILAQLLVMLFRFYAEQPRKDNQTVMRIADALSFLEAHPDRSVSTSELMELTNMSAST
                     LNRHFKNATGLSPIEFHIQRRIKHACRLLRSSDMSVSEISEATGFEDSNYFSRQFRNV
                     MGITPLSYRKNRSMWYRLP"
     gene            complement(1819..2244)
                     /gene="fucU"
                     /locus_tag="PLF67_09585"
     CDS             complement(1819..2244)
                     /gene="fucU"
                     /locus_tag="PLF67_09585"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HOE90301.1"
                     /translation="MLKTIPSILSPELLKVLMEMGHGDELVIGDGNFPAASMNDRVVR
                     LDGHGGEEILKAILQLFPLDTYAPNAFLMEKVPGDPAETPIWDSYEKIAKAGDPGFTG
                     FTFVERFAFYEQSKKAYAIVATGESALYANIILKKGVVL"
BASE COUNT          498 a          694 c          610 g          553 t
ORIGIN      
        1 tcagcagttc atcatcaaac aggaggatcg aactgagctt gtcggagaca tcctcctcgg
       61 tgtggaagtg ccccatgtcg aggcagagca tcaccccgtt ccgggcggca tagttcatgt
      121 agaactcgtg cgagccgacg acgaacgctt cgctgccgat gccgaagagc ttggtctcaa
      181 gggcgtcccg catatgctcg cggggatact cggtggtgaa aatctcatca aggctctcct
      241 tgaggatcgc ccgatagccc atcttgtcgg cggtatggtt tttcgccccg tcgggaatcc
      301 acgtattgag aatgcacggc gatccctgct gctttccgat ccacgctgcg atcttccggg
      361 ttcgtttggc atgctcgatc cagaactgac gaatctcttc atccttgctt gccagggtat
      421 agccatcatt cgccatgggg tggctgaaaa acgttccgtt gaaatcaagg tggacgttgt
      481 tcgcttgcgc ccaatccacc catccctgaa agtgtttcgg ttcaatctcg tttcggtcga
      541 cgaactcgga gccgaactcg ccgtagctcg catggaggct gatcctatgc ggaccaggga
      601 cgatcgaaag cacctcttca agatcggctc tcagctgctc gatcgtctgc gctttgccgg
      661 gataggagcc ggtgacctga attccaccgc cgctgagctc ggcatcggga gtttcaaacc
      721 cgccgacatc atcgccctgc cagcaatgaa tcgagatcgg aatccgatca agccgcttga
      781 ggactgcatc ggtatcaacg ccgatcgttc ggtaccgttc tttcgcgtac tgatatgaag
      841 tggtttccat acactcctcc tatctataat gttaccccca ctttaccatt tttgtcttga
      901 cataataggc atattttagc actattttgt cataatgagc ttagtactcc gggcttccga
      961 ctatttttac gacccttcct tccccatcaa ggtccttgaa cgggatccgg aaattcccta
     1021 tccccttcac agccacgact tctgggaact gatcgtcatc gtctcgggaa gcgggaccca
     1081 cttcgacacc gccggatcgt tcgagctgac cagtggaaac gtcttcgtca ttcctcccgg
     1141 ctttaaacac ggctatcggg atgtccgcga tctgcgcctc ttcaacatcc tcttcgacat
     1201 ctcgatgttc gaggaatcat ttttcgattt gagcaacatg agcggctacc aggcgctttt
     1261 gaaggtggag ccctcctacc gccatgacgg ctcgctttcg acgctgatgc agctcacccc
     1321 cgcccagcag gcgcgggttc ttccccttct tgaacacatg ctcgtggaat cggattcgac
     1381 cgaaagcgag atcggggcga aggccggagc gttcgcgatc cttgcgcagc ttctggtgat
     1441 gctgtttcgc ttttatgccg agcagccgcg gaaggacaac cagaccgtca tgcggatcgc
     1501 cgacgccctc agctttttgg aagcccatcc cgaccgctcc gtttcaacga gcgaattgat
     1561 ggaactgacc aacatgtcgg caagcaccct caaccggcac ttcaagaacg cgaccggcct
     1621 ctcccccatc gaattccaca tccaacggag gatcaagcat gcgtgccggc ttctccgctc
     1681 aagcgacatg tcggtcagcg agatcagcga agctacgggg ttcgaggact ccaactactt
     1741 cagccggcag tttcgcaacg tgatgggaat cacgccgctc tcctatcgga agaaccgctc
     1801 gatgtggtat cggctccctt agagaacgac gcctttcttg aggatgatgt tcgcgtacag
     1861 ggcgctttcg ccggtcgcca cgatcgcata cgccttcttc gactgctcat agaacgcaaa
     1921 gcgctcgacg aaggtgaacc cggtgaagcc cggatcaccc gctttggcga ttttttcata
     1981 ggaatcccag atcggggtct cggcggggtc gccgggaacc ttttccatga gaaacgcgtt
     2041 gggagcgtag gtgtccaagg ggaaaagctg caggatcgct ttcagaatct cctcaccgcc
     2101 gtgaccgtca aggcggacga cccggtcgtt catcgatgcg gcggggaaat ttccgtcgcc
     2161 gatcaccagt tcatcaccgt gccccatctc catgagaacc ttcagcagct cgggactgag
     2221 aatcgaggga attgttttaa gcatggtatg ccactccttt tttttcgttg caatcgcttg
     2281 gatgctgtgc gatactgaga gtatgtaaac gattacaaaa ccatcataca ccaaggagaa
     2341 cgagatggca aacat
//
LOCUS       DAOSLI010000131         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_48965_length_1995_cov_12.696392, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000131 DAOSLI010000000
VERSION     DAOSLI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48965_length_1995_cov_12.696392"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLF67_09590"
     CDS             complement(<1..373)
                     /locus_tag="PLF67_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90302.1"
                     /translation="MKKAATHVGITIVVVGLMIASIIYLMSNSLFTVTRFGTNYLNRV
                     FSYQVTTLALSLVIILIIAFLTRFQSLRLLSVTRIDGEVHPEPWIGISTRNKDTWKTL
                     GRNVAIVISVVTAIAIYFQVYR"
     gene            complement(370..888)
                     /locus_tag="PLF67_09595"
     CDS             complement(370..888)
                     /locus_tag="PLF67_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOE90303.1"
                     /translation="MSTKCALLGLLNIRKMTAYELVKFANESIGFFWNESYSNVHRTL
                     NLLAEENLIEKLPEKGSRNKNIYAINDRGRQALSEWLSNHQHTTIYRDELLLKLFVSS
                     RKDYPALLVDLKNVFEELQMQRQVYAGLQELLSSRVDNLSFDLVLEYGIVQNEVTIEW
                     LKKTIQTIEEII"
     gene            complement(900..1937)
                     /locus_tag="PLF67_09600"
     CDS             complement(900..1937)
                     /locus_tag="PLF67_09600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOE90304.1"
                     /translation="MTEKIEQLLQNSAKKSKTLQFAMHLPESGTRYSFSSTKPDQRFH
                     SASVGKLMTSTLIFMAIEQGTLSLDAKVAGILKNGMLDKLFVFEDVDYQGEVTIRQLL
                     GHTSGVNDYFGSKTFNGSSFTEDMIRNPDTFWTPDMMLDFTRIRQKALSRPGEKFFYS
                     DTGFVLLGLVIEEVFKMPFYQALETYLFKPAGMKDTHLCFYSEGFDQKALGPTSINGV
                     DVHLFRSLSADFSGGGLSTTTEDLLKFLDALQNGKFISQSSLDLASGFTNRYRQGLHY
                     GLGMMQIRFEEFFFMLKNLPRVQGHLGVTGVHAWYDPKSKGMFVLNVGNTKDMALSFR
                     LLIKILQIVYR"
BASE COUNT          472 a          541 c          492 g          490 t
ORIGIN      
        1 tgcggtacac ctgaaaatag atcgcaatcg ccgtcacgac cgagatgacg atggctacgt
       61 tccgccccaa cgtcttccag gtgtccttgt tcctggtaga gatcccgatc cagggttcgg
      121 ggtgaacctc gccgtcgatc ctcgtgacgg acaacagtcg aagcgattgg aagcgggtca
      181 gaaaggcgat gatcaggatg atcaccaagc tcaaggcaag cgtggtcact tgatacgaga
      241 atacccgatt gagatagttc gttccgaacc gggtaacggt gaacagcgaa ttgctcatca
      301 gatagatgat gcttgcaatc atcaatccca ccacaacgat ggtgatacct acatgggttg
      361 ccgctttttt catatgattt cctcaatggt ttgtatcgtt tttttaagcc actcgatcgt
      421 cacctcgttt tgcacgattc catactccaa cacgaggtcg aatgagagat tgtccaccct
      481 cgacgataga agctcctgca atcctgcata cacctgtctt tgcatctgca gttcctcaaa
      541 aacgtttttg aggtcgacga ggagagccgg atagtctttt cggctgctga caaagagctt
      601 gagcagaagc tcatcccggt agatcgtcgt atgctgatga ttcgacagcc actcggacag
      661 cgcctgccgc ccccgatcgt tgatcgcata gatgttcttg tttctgcttc ccttctccgg
      721 cagcttttcg atgaggttct cttcggcaag gaggttcagc gtccggtgga cgttgctgta
      781 cgattcattc cagaagaacc cgatcgattc attggcgaac ttcaccagct cataggcggt
      841 catctttcgg atgttcagaa gaccgagcaa agcacatttc gtcgacacga cctctcctcc
      901 tatcgataca cgatctgcag gatctttatc agcagtctga aactcaaagc catatccttg
      961 gtgttgccga cattcaggac gaacatccct ttgctcttcg gatcatacca ggcatgaact
     1021 cccgtcaccc cgagatggcc ctgcactctc ggaaggtttt taagcatgaa gaagaactcc
     1081 tcgaagcgga tctgcatcat cccaagtccg tagtggagcc cctgacgata gcgatttgtg
     1141 aagcccgaag ccagatcaag cgaggattgg ctgatgaact tcccgttctg tagcgcatcg
     1201 aggaacttca agagatcctc cgtcgtcgtc gagagtccgc ccccggagaa atcggcactg
     1261 agacttctga acagatgcac gtcaacaccg ttgatggagg tcggccccag cgccttctgg
     1321 tcgaagcctt cgctgtagaa gcacaggtgg gtatccttca tgccggccgg tttgaagaga
     1381 tatgtctcga gcgcctgata aaacggcatc ttgaagacct cctcgatgac cagtccaagc
     1441 aggacaaacc cggtatcgga gtagaagaac ttctcccccg gtctggaaag cgccttttgc
     1501 cggattctcg taaaatcaag catcatatca ggcgtccaga acgtgtcggg attcctgatc
     1561 atgtcctcgg tgaaggaaga tccgttgaag gtcttgcttc cgaagtaatc gttcactccg
     1621 gaggtgtgcc cgagcagctg ccggatcgta acctccccct gatagtccac atcctcaaag
     1681 acgaacagct tgtccaacat cccgtttttc aggatgccgg caaccttggc atcgagagac
     1741 agcgttccct gctcgatcgc catgaaaatc agcgtgctcg tcatgagctt gccgacgctt
     1801 gcgctgtgga agcgctggtc gggtttcgtg cttgagaaag aataccgtgt cccggattcc
     1861 ggcagatgca tggcgaactg aagcgtcttg ctcttctttg cgctattctg caatagctgt
     1921 tctatttttt cagtcatcaa catgatccaa gctcctaaaa aagaatctta agcaaacgat
     1981 acatatatct attag
//
LOCUS       DAOSLI010000132         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_50090_length_1963_cov_25.623690, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000132 DAOSLI010000000
VERSION     DAOSLI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50090_length_1963_cov_25.623690"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..903
                     /locus_tag="PLF67_09605"
     CDS             19..903
                     /locus_tag="PLF67_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE90305.1"
                     /translation="MRYWQLYAMLIIPLLYFLLFKYVPLFGNVLAFRRYRPGMGPFGT
                     EWVGLHYFQRFWGDPAFWRAFRNTLVLSLLNLVVNFPIPIVFAILLNEVRILPFKKAV
                     QTVSYMPRFISTVVVIAILGELLSPSTGIVNIIRMNVFGQKAIYFINEAKYFRWIYIF
                     VDTWQYTGWTAIIYLAAITAIPADLYEAATIDGATRIQQIFYVTIPSIMPTIMVMLIL
                     NVGRLLSLGFEKVLLLYTPDNSMVSDIIDTLVYRTGLANQNYSYATAIGLFSGIIGVI
                     LVSSSNFLSRKITGEGVY"
     gene            903..1772
                     /locus_tag="PLF67_09610"
     CDS             903..1772
                     /locus_tag="PLF67_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE90306.1"
                     /translation="MKSYSDRSTILFQILVYTVMIFTLLVTLIPLISMAALSLSSSKA
                     IVNNEVFLWPKGLTFEAYRQIFTYPNFFRAYGNTFFYTFFGTAISLVVMIFFAFPLSK
                     PALKGTKFVLKLVVFSMFFSGGLIPNYLLISNLGLTNTRWAILLPFAINQFNLILLIN
                     FFKSIPIDLEEAAIIDGVNYAGILTRIVLPLSGAALATVGLYTAVFFWNDWFNGLIYL
                     KSSQFPVMLFLRNIVTGTSMVGEAAGVGDKSAIAISIKSAVIVTSTLPIILLYPFLQR
                     YFVKGVMIGAVKG"
BASE COUNT          426 a          516 c          474 g          547 t
ORIGIN      
        1 aaagcaaaag catatttctt gagatactgg caactatacg cgatgttgat catcccgctc
       61 ttgtattttc ttctctttaa atacgtcccc ctctttggaa acgtgttggc gtttcggcgc
      121 tatcgaccgg gaatgggtcc ctttgggacg gagtgggtgg gactgcacta ttttcagcgg
      181 ttctggggcg atccggcgtt ctggcgggcg tttcgaaaca cgctggtatt gagtctgctc
      241 aatctggtgg tcaatttccc cattcccatc gtctttgcga ttctgttgaa cgaggttcgt
      301 attcttccct tcaaaaaagc ggttcagacc gtgtcgtaca tgccgcgttt catctccacg
      361 gtcgtcgtga tcgcaattct gggtgagctg ctttcccctt ccacgggaat cgtgaacatc
      421 atacggatga acgtcttcgg acaaaaggcg atctacttca tcaatgaagc caagtacttc
      481 cggtggatat acattttcgt cgacacctgg caatacaccg gatggacggc gatcatctat
      541 cttgccgcga tcacggcgat tcccgccgat ctgtacgagg cggccacgat cgacggagcc
      601 acccggatcc agcagatctt ctacgtaacg atcccttcga tcatgccgac gatcatggtg
      661 atgctcatat taaatgtcgg ccggctgttg tcactgggct ttgaaaaggt tcttctgctc
      721 tatactccgg acaactcgat ggtcagcgac atcatcgaca cgctggtgta ccgaaccggc
      781 cttgccaacc agaactactc ctacgctacg gcgatcgggc tcttcagcgg aatcatcgga
      841 gtgattctgg tttcctcttc gaacttcctc agcaggaaga tcaccggtga aggagtgtac
      901 tgatgaaatc ctacagcgac cgttcaacga ttctttttca gattctcgtc tacaccgtga
      961 tgattttcac ccttttggtc accttgattc cccttatttc aatggcggct ctttcgcttt
     1021 caagctcgaa ggcgatcgtg aacaacgagg tgttcctgtg gcctaaaggc ctcacctttg
     1081 aagcatatcg acagattttc acctacccga acttcttccg ggcgtatggc aatacgttct
     1141 tctatacgtt cttcgggacg gcgatcagtc tggtggtgat gattttcttc gcgtttccac
     1201 tgagcaaacc cgcgttgaaa ggaacaaagt ttgtattgaa gcttgtggtc ttttcgatgt
     1261 ttttctccgg aggcttgatc cccaattacc tgttgatctc caatctcggc ttgaccaaca
     1321 cccgttgggc gattctcctg ccgtttgcga tcaaccaatt caacctgatt cttctcatca
     1381 acttcttcaa gtcgattccg atcgacttgg aggaagctgc gatcatcgac ggggtgaact
     1441 acgccggtat tctgacaagg atcgtgcttc cgctctccgg tgccgcgttg gcgaccgtcg
     1501 gactgtatac cgcggtattc ttctggaacg actggttcaa cggcctcatc tacctcaaga
     1561 gcagtcaatt cccggtcatg cttttcctcc gaaacatcgt taccgggacc agcatggtcg
     1621 gcgaagctgc gggagtgggg gataagagcg ccatagcaat ttcgatcaaa tccgcggtca
     1681 tcgtgaccag cacccttccc atcatcctcc tctatccctt cctccaacgc tacttcgtga
     1741 agggcgtgat gatcggagcg gtgaaggggt aatctcaaaa gagagtccgg aaatgtgacc
     1801 tcatgtaaaa gttgtcgttt ttgcaggagt ggaatcttcg agtccctgta atcaataact
     1861 cgtataattt gcaaaaagca tggaaacaat tgcgtattgt ggcggtttcc tcatgtatgg
     1921 tagcataact agtgataaag agagaccgct ctcttgattt aat
//
LOCUS       DAOSLI010000133         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_54040_length_1860_cov_10.274792, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000133 DAOSLI010000000
VERSION     DAOSLI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54040_length_1860_cov_10.274792"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1860)
                     /locus_tag="PLF67_09615"
     CDS             complement(<1..>1860)
                     /locus_tag="PLF67_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE90307.1"
                     /translation="AYRYIMIDEFQDNNEEQKRLLYLLAEKEGFEADGIPTVTDLSQD
                     KLFFVGDEKQSIYRFRGSDVSVFKALAKELQGIGGMSVKLPRNYRSEPGLITWFNEIF
                     PSIMKNEGEPFEADFEPLEFREPKEGIDSKITLAIKPYVAGAEPGEEELAEDSLAEAY
                     HLAAKIDHILNGDEYLIPIKNGSPRKPKPNEIAILMRTTSNQLHLERALRLFSIPYTV
                     VMARSFFLEAITNDFYAMLQLLIHPSDRLSYAVVLRSPFCFIDDSALVELLQSEELFT
                     VVDTLSERDRSKLLQMKLFFEDLKEAATVQRLEHLIFMLWHESGYAHHYIQNRHYHAY
                     IEHYELIYRLAQKFQEEGRTLSEFLDYLRERLGRNERLEEMDLIREKEEGIQIMSVHQ
                     AKGLEFPIVILANAGGGIRNQGKKLSTILGYSLPNYLDRVNPRRKKDRIKNVDALFDE
                     GEEAAQELAELKRLLYVALTRAETHLIISGSFSEKKRNIDGKERTFLLLLCQALGIDP
                     YDPTLEHGKVLFELIEDVPSDVFYRDLEADVPIEELKPWYESVDAPYQAEALRYAVTA
                     LAPEVERTFVKELPAIASDIFLGEKECSALFGTFVHRLIEAHLKKEDLSDPLF"
BASE COUNT          398 a          514 c          507 g          441 t
ORIGIN      
        1 agaagagcgg atcggaaagg tcttcctttt taagatgggc ttcgatcagg cggtgaacga
       61 acgttccaaa caaagcggag cattcctttt cgccaaggaa gatgtcgctt gcgatcgccg
      121 gcagctcctt gacgaacgta cgttcaacct cgggagcgag agccgtcacc gcataccgga
      181 gcgcctccgc ctgataggga gcgtcaacgc tctcatacca gggcttcagc tcctcgatcg
      241 gcacgtctgc ttcaagatct cgatagaaca catcggaagg aacatcttcg atcagttcaa
      301 agagaacctt cccgtgttcc aatgtcggat cgtacgggtc gattcctagg gcctggcaga
      361 gaagcaggag gaacgtccgc tcctttccat caatgttccg ttttttttca gaaaagctcc
      421 cgctgatgat caggtgggtc tccgcccggg tgagggcgac atagagcagc cgcttcagct
      481 cggcaagctc ctgggcggcc tcctctcctt catcaaacag ggcatcgaca ttcttgattc
      541 gatccttttt ccttctgggg ttcactctat ccagatagtt cggaagagaa taccccaata
      601 tggttgagag tttctttcct tgattacgaa ttccaccccc ggcattggcg aggatgacga
      661 tcgggaactc aaggcccttg gcctgatgca ccgacataat ctggatcccc tcttcttttt
      721 ccctgatcag atccatctct tcaaggcgct cattccgtcc gaggcgctcc cgaagataat
      781 cgaggaactc gctgagcgtc ctgccttcct cctgaaactt ttgggcgagt cgatagatca
      841 gctcgtagtg ctcgatgtag gcgtggtaat ggcggttctg tatgtagtgg tgggcgtagc
      901 cgctttcatg ccacagcatg aatatgaggt gctccagacg ctggacggtc gccgcctctt
      961 tcagatcctc aaagaagagc ttcatctgga gaagtttgct ccggtcgcgc tcggaaaggg
     1021 tgtcgacgac ggtgaagagt tcctcgcttt gaaggagctc aaccaaggcg ctgtcgtcga
     1081 tgaagcagaa cggcgagcga agcaccaccg cataggagag ccggtccgac gggtggatca
     1141 gaagctgcag catggcgtaa aagtcgttgg tgattgcctc cagaaagaag gagcgggcca
     1201 tcaccaccgt atagggaatc gaaaacagcc tcagagcccg ctcaagatgg agctggttcg
     1261 aggtggtgcg catgagaatc gcgatctcgt tcggcttagg ctttctggga gatccatttt
     1321 ttatgggaat gagatactcg tcgccgttga ggatgtgatc gatctttgca gccagatgat
     1381 acgcctcggc gagggaatcc tccgccagct cctcctcgcc cggttcggca cccgctacat
     1441 atggcttgat cgccaaggtg atcttcgaat cgattccttc ctttggttcc cggaactcca
     1501 aaggctcgaa gtccgcttcg aagggttcgc cctcgttctt catgatcgac ggaaaaatct
     1561 cgttgaacca ggtgatcagg ccgggttcgc tccggtagtt tctgggcagc ttgacgctca
     1621 tccccccgat cccctgaagc tccttcgcca gagccttgaa cacggagaca tcgctgcccc
     1681 ggaagcggta gatcgactgc ttctcatctc cgacgaagaa gagcttgtcc tgcgaaagat
     1741 cggtcacggt aggaatgcca tccgcttcga atccctcttt ttccgcaagg agatagagca
     1801 accgtttctg ctcctcgttg ttgtcctgga actcgtcgat catgatgtag cgatatgcct
//
LOCUS       DAOSLI010000134         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_56448_length_1801_cov_28.091065, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000134 DAOSLI010000000
VERSION     DAOSLI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56448_length_1801_cov_28.091065"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..>1801
                     /locus_tag="PLF67_09620"
     CDS             45..>1801
                     /locus_tag="PLF67_09620"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOE90308.1"
                     /translation="MANILDIKQVGPEKRYASELPKIGIRPVIDGRQRGVRESLEEQT
                     MNMAKAAAKLISDNLRHTNGEQVECVIADSTIGGVRQSADCAEKFKKEGVAVTLTVTP
                     CWCYGTETFDMDPLTVKAVWGFNGTERPGAVYLAAVLAAYTQKGLPAFGIYGRDVQDS
                     TDTSIPEDVQEKILRFAKAGLAVAEMRGKSYLSIGGMSMGIAGSIVDQDFLQAYLGMR
                     TEVVDMSEIERRIAEEIYDPVEFKKAIEWVRKHVIQAEDTVNDPALRKSQEELDKDWE
                     YVTKMVMIGRDLMIGNPKLNTLGFKEESYGRNAIAAGFQGQRQWTDHWPNGDFMETML
                     TSSFDWNGIREPYIMATENDHLNGISMLFAKLLTNRAVIFSDVRTYWSPKSVERVTGW
                     KPTGLAKDGFIHLINSGATTLDAAGQMANEKGEPAMKPYWEITAEDVQKTLDNTRFSV
                     GNLGYFRGGGFSSTFLSEGGMPVTMLRLNMVKGVGPVLQIAEGWTADVPEEVFNIINK
                     RTDQTWPTTWFVPRLVEHEGPFKDVYSVMANWGANHGAIAYGHVGADLITLASMLRIP
                     VNMHNVAEKDIFRPSAWSML"
BASE COUNT          420 a          522 c          550 g          309 t
ORIGIN      
        1 gtatgtaaac gattacaaaa ccatcataca ccaaggagaa cgagatggca aacattcttg
       61 atatcaaaca ggtggggccg gagaagcgct acgcttcaga gcttccgaag atcggaatcc
      121 gtccggtcat cgacgggcgc cagcggggcg ttcgcgagtc gctggaagag cagacgatga
      181 atatggccaa ggccgcagcg aagctcatca gcgacaatct tcgccacacg aacggtgagc
      241 aggtggagtg cgtcatcgcc gactccacga tcggcggagt gcgccagagc gccgattgcg
      301 ccgagaagtt caaaaaggaa ggggtcgctg taaccctgac cgttaccccg tgctggtgct
      361 acggaacgga gaccttcgac atggatccgc tgacggtcaa ggcggtctgg ggcttcaacg
      421 gcaccgaacg ccccggcgcg gtgtaccttg cggcggttct tgccgcctac acccagaaag
      481 gcctccccgc cttcggcatc tacggccggg atgttcagga ctcgacggac acctcgattc
      541 ccgaggatgt ccaggagaag attctccgct tcgccaaagc cggtctggcg gtcgccgaga
      601 tgagaggaaa gagctacctc tcgatcggag ggatgtcgat gggaatcgcc ggctcgattg
      661 tcgaccagga tttccttcag gcgtaccttg gcatgcgaac cgaagtcgtg gacatgagtg
      721 aaatcgagcg gcggatagct gaagagatct atgatcccgt cgagttcaag aaagcgatcg
      781 aatgggtccg caagcatgtc atccaggcgg aggacaccgt caacgatccc gccctccgca
      841 agagccagga agagctggac aaggattggg agtacgtcac caagatggtg atgatcggcc
      901 gggacctgat gatcggaaac cccaagctca acaccctcgg attcaaagag gagtcctatg
      961 gccgcaatgc gatcgccgcg ggcttccagg gccagcgcca atggaccgac cactggccga
     1021 acggcgactt catggagacg atgctcactt ccagcttcga ctggaacggc attcgggagc
     1081 cgtacatcat ggcgaccgag aacgaccacc tcaacgggat cagcatgctc tttgcgaagc
     1141 tcttgaccaa cagggcggtg atcttcagtg acgtgcggac gtactggagt cccaagagcg
     1201 tcgaacgggt aaccggatgg aagcccaccg ggttggcgaa ggacggattc atccacctga
     1261 tcaacagcgg agcgacgacc ctcgacgccg ccggacagat ggccaacgaa aagggtgagc
     1321 cggcgatgaa gccctattgg gagattaccg ccgaggatgt ccagaagacg ttggacaaca
     1381 cccgcttcag cgtcggaaac ctcggatatt tccggggcgg cgggttctcc tccaccttct
     1441 taagtgaagg cggaatgccg gtgacgatgc ttcggctgaa catggtcaag ggcgtcggcc
     1501 cggttcttca gatcgccgag ggatggacag ccgatgttcc cgaggaagtc ttcaacatca
     1561 tcaacaagcg aaccgaccag acctggccca ccacctggtt cgttccccgc ctcgtggagc
     1621 atgaaggccc cttcaaggat gtctactccg tgatggcaaa ctggggagca aaccacgggg
     1681 cgatcgccta tggccacgtc ggcgccgacc tgatcaccct cgcttcgatg ctccggatcc
     1741 cggtcaacat gcacaacgtg gcggaaaagg atatcttccg ccccagtgcc tggtcgatgc
     1801 t
//
LOCUS       DAOSLI010000135         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_58255_length_1761_cov_29.442556, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000135 DAOSLI010000000
VERSION     DAOSLI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58255_length_1761_cov_29.442556"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..82)
                     /locus_tag="PLF67_09625"
     tRNA            complement(10..82)
                     /locus_tag="PLF67_09625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Ala,seq:cgc)
     gene            186..1103
                     /locus_tag="PLF67_09630"
     CDS             186..1103
                     /locus_tag="PLF67_09630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE90309.1"
                     /translation="MHEEYRDDILSSVMNTIADPILVVDEDGRYLEVFGGTDRSLYDD
                     GRPLKGKTVSEILTKEFGQFFVNQVKHTIAMGTLNVFDYRLDTQTVNLPIFDGPGGTQ
                     WFEARMYPLEQPIEGKRAVVSMIINISERRALHRRLHELSYKDPLTGLYNRRYFLERV
                     NVHLLEKNSIHLMFGDIDHFKAINDRYGHLAGDEVIRELAAILQSILGETTTICRLGG
                     DEFIAALVNSTDEQTLELAEKVRSTVEDYRFMYQDTPIEFTVSIGIAPVDDDSLDSAT
                     LIGHADKALYEAKEAGRNCVRLYNKPPRG"
     gene            1110..>1761
                     /locus_tag="PLF67_09635"
     CDS             1110..>1761
                     /locus_tag="PLF67_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HOE90310.1"
                     /translation="MVHLVININLLLTMSILIGLYPFKLNNWWDRHKMVRGVIIGLLG
                     MLIMSFPFIYTEGIIFDTRTILLAVSGMVFGLVPTLVAAIMMIIFRVMMGGAGVVMGI
                     STILVSSTIGIGWHLLRFEAVDDVKPPLLEFLVVGFISQFFSWLCLFLLPRSEWEAVL
                     KMITIYFLVIYPFFMAALGIVLLSQIQRITAMREIARSEFILKTIFDDAPIGMSRTD"
BASE COUNT          382 a          457 c          492 g          430 t
ORIGIN      
        1 cccgtctgtt ggagccaatg ggactcgaac ccaccacctg ctgattgcga accagccgct
       61 ctaccagatg agctatgacc ccgtatgtaa gagtcaaaat atctcagtgg cgaaaaactg
      121 tcaactcttt tgcttacagg gaggctcttc tgtgatagag ttaggcagag gtggaggaat
      181 ctgttatgca tgaagagtat cgggatgaca tcttgtcctc ggtgatgaat acgatcgccg
      241 atcctatcct ggttgtcgat gaggatggga ggtatcttga ggtctttggc ggaaccgatc
      301 gttccctcta tgatgacggg cggccgctga agggaaagac ggtttcagag atactgacca
      361 aggagttcgg tcagttcttc gtgaatcagg tcaaacacac gatcgccatg ggtacgctga
      421 atgtgttcga ctatcggctc gacacccaga cggtgaatct gccgatcttt gacggaccgg
      481 gaggaacaca gtggttcgag gcgcggatgt acccgcttga gcagccgatc gaaggaaaac
      541 gggcggttgt ctcgatgatc atcaatatct ccgagcgacg ggcgctccat cggaggcttc
      601 atgagctctc ctataaggat cctttgacgg ggctgtacaa tcggcggtat ttccttgagc
      661 gggtgaacgt ccatctcctg gaaaagaatt cgatccatct gatgttcggc gatatcgatc
      721 acttcaaagc gatcaatgac cggtatggac accttgccgg tgatgaggtg attcgggagc
      781 ttgccgccat tctgcagtcc attctcggtg aaacgactac catctgccgc cttggcggcg
      841 atgaattcat cgccgcattg gtaaactcca cggatgagca aacccttgaa cttgctgaga
      901 aggtacgcag taccgtggaa gattatcgct tcatgtatca ggatacgccg atcgaattca
      961 ccgtctcgat cgggatcgct cccgtggatg atgacagcct tgacagcgcc acgctcatcg
     1021 gccatgcgga taaagctctg tatgaagcaa aggaggcggg tcgaaactgc gtccgcctct
     1081 acaacaagcc gcccagggga tgatggaaga tggttcacct cgtcatcaac atcaacctcc
     1141 ttctcacgat gagcattctc atcggactct atccgttcaa actgaacaac tggtgggatc
     1201 ggcataaaat ggtgcggggc gtcatcatcg gtcttctggg catgctgatc atgagctttc
     1261 ccttcatcta tacggaagga atcatcttcg acacccggac gattcttttg gcggtgagcg
     1321 ggatggtgtt cggactcgtc ccgactctcg tcgccgccat catgatgatc atcttccggg
     1381 tcatgatggg cggagcgggg gtcgtgatgg gcatctcgac gatcctcgtc tcctcgacga
     1441 tcggaatcgg ttggcatctc ttgcggttcg aagcggtgga cgatgtgaag cccccgcttc
     1501 tggagttcct cgtggtgggt ttcatttcgc agttcttctc ctggttgtgc ctgttccttc
     1561 tgcccagaag cgagtgggaa gccgtcctca agatgatcac gatctacttt ctggtgatct
     1621 atccattctt catggctgcg ctggggatcg tccttctctc ccagatccaa cgaatcacgg
     1681 cgatgcgtga aatcgcccgc agcgagttca tcctgaagac gatctttgac gatgctccga
     1741 tcggcatgag ccgaacggat c
//
LOCUS       DAOSLI010000136         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_58515_length_1755_cov_24.475882, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000136 DAOSLI010000000
VERSION     DAOSLI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58515_length_1755_cov_24.475882"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLF67_09640"
     CDS             <1..497
                     /locus_tag="PLF67_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOE90311.1"
                     /translation="GVAWKMAIKRSDGPTALLLSRQNLVPRKRTEEQLSLIEKGGYIL
                     HETDSSPRVILIGTGSEVELAVKAAKVLEEEKISVRVVSMPSPEVFEAQDSKYKEGVL
                     PKAVKARVAVEAGWADYWYKYVGLDGKIIGMRSFGESGPAEQLYEHFGITVDKVVEAA
                     KSLL"
     gene            547..1134
                     /locus_tag="PLF67_09645"
     CDS             547..1134
                     /locus_tag="PLF67_09645"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE90312.1"
                     /translation="MSDYYETNAASYIEETKNLDMSRFTSRFLRSIPEGGAILDAGCG
                     SGRDALLFHEKGYRVRAFDASKELVRLARQWTNLPIEHRSFHEMNEYAEYDGIWASAS
                     LLHLACDELPTVFSLLYRALKEQGILYASFKVGESFTKEGLLYTSFIPDEFRSFIRNE
                     TSFIIDELIISEDAREYRKGEFWLNAFLKKNATVS"
     gene            complement(1159..1638)
                     /locus_tag="PLF67_09650"
     CDS             complement(1159..1638)
                     /locus_tag="PLF67_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOE90313.1"
                     /translation="MDVLDVAIRLEQEGAKFYEELTKNAPTQGFATIFTMLAEDERKH
                     ERYFKALKEKHKPITASTMIIDEARKIFQEFDPSEFPEERDQIPAYERAIEIEQKSID
                     LYREQVSISTDESEKHLLNIIIEEEERHKRVLTELLGLVVRPHRWVEDAEFGIREEY"
BASE COUNT          401 a          442 c          486 g          426 t
ORIGIN      
        1 cgggggtcgc ctggaagatg gcgatcaagc gctcggacgg accgacggcc ctgctccttt
       61 caaggcagaa tctggttccc cggaagcgga cggaagagca gctttccctg atcgaaaagg
      121 gtggatatat cctccatgaa accgacagca gcccgcgggt catcctcatc ggaaccggta
      181 gcgaggtgga gctggccgtc aaggcggcca aggtgctgga agaagagaag atctccgtcc
      241 gggtcgtctc gatgccctcg ccggaggtct tcgaagcaca ggactccaag tacaaggaag
      301 gtgttctgcc caaggcggtc aaagcccgcg tcgccgtcga agccggctgg gccgactact
      361 ggtacaagta tgtcggtctg gatgggaaga tcatcggaat gcgctccttc ggagaatccg
      421 gtcccgccga acagctgtat gaacacttcg gcatcaccgt tgataaggta gtcgaagcgg
      481 cgaagagtct gttgtaagca tgaagaccaa tcaatcctcc ggcgagcgtc ggaggatcgt
      541 tttcccatga gcgactacta cgaaaccaac gccgcctcgt acattgaaga aaccaaaaac
      601 cttgatatga gccgctttac aagcaggttt cttcgatcga ttcccgaagg cggagcgata
      661 ttggatgcgg ggtgcgggtc gggtcgagat gcccttctgt ttcatgagaa gggctatcgg
      721 gtaagggcgt ttgatgcctc taaggagctt gtccgattgg ctcgtcagtg gacgaatctc
      781 cccattgagc atcgaagctt ccatgaaatg aacgaatatg ctgaatatga cgggatctgg
      841 gcttccgctt ccttgttgca tctggcctgc gatgagctgc cgacggtctt ttcgctgctg
      901 tatcgggcat tgaaagaaca ggggatcctg tatgcttcct tcaaggtggg agaaagcttt
      961 acaaaagagg gacttctcta tacctccttc atcccggatg agttcagatc gttcattcgg
     1021 aatgaaacct ccttcatcat cgatgagctg atcatctccg aggatgcgag ggaatatcgg
     1081 aaaggggagt tttggttgaa tgcgtttttg aaaaagaatg ccaccgtctc ctgaacagga
     1141 agcggtggcg gattccgatc aatactcttc ccggataccg aactcggcgt cttcgaccca
     1201 gcgatggggt cgaacaacca gaccaagcag ctcggtcaga actcgtttgt gtcgttcctc
     1261 ttcttcaatg atgatgttga gcaggtgttt ttcactctca tccgtagaaa tggaaacttg
     1321 ttcgcgatac aggtctatcg atttctgttc aatctcgatc gctcgctcat aggcggggat
     1381 ctggtcccgc tcctccggaa attcggaagg atcgaactcc tggaagattt tccgggcttc
     1441 atcgatgatc atggtcgaag cggttatggg cttgtgcttt tccttcaacg ctttgaagta
     1501 gcgttcatgc ttgcgctcat cttcggcgag catcgtgaag atggtggcaa atccctgcgt
     1561 cggagcgttc tttgtcagct cctcatagaa ttttgcacct tcctgttcca gccggatagc
     1621 gacgtccaat acgtccatgg cttcctcctt taattaccta caatataact aggaattggt
     1681 gactatgcaa cgcttcacca ttttaccagc ggaatcgagg tcttgatgaa gcggaagaat
     1741 cactctgaaa acgct
//
LOCUS       DAOSLI010000137         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_60104_length_1723_cov_22.108513, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000137 DAOSLI010000000
VERSION     DAOSLI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60104_length_1723_cov_22.108513"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLF67_09655"
     CDS             complement(<1..473)
                     /locus_tag="PLF67_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE90314.1"
                     /translation="MSPVNDREVVLDVQNLKTYFKTDAGIVKAVDGVSFTLHKGQTLG
                     IVGESGCGKSVTNLSIMRLIPSPPGKVVGGEAIFEGTDILALDEKEMRTIRGNKISMI
                     FQDPMTSLNPFLRISTQMIETIRLHTKGISKKEALERSIEMLKLVGIPSAEQRVHA"
     gene            complement(486..1535)
                     /locus_tag="PLF67_09660"
     CDS             complement(486..1535)
                     /locus_tag="PLF67_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE90315.1"
                     /translation="MSYEEISQIYADLSKTDIEAVKAQTLTEVTSQLMNTLKSATPGL
                     SAEEYEVNLELELQTIGESGIAKLGKTNLLGKISTTITRQADRDLKQEIKEGLAVFPV
                     ERIIDVNEQLQAKVTATKKHERMYYLGTDYSGRDMLSRIIYGSQVSIAIGLVGTLTSV
                     LIGIILGAVAGYVGGKVDYILMRIVDIMYGLPYMLLVIIFMAIFGRNILNLFGALALV
                     SWLTIARMVRGQVMSLKNSEYVEAARSMGGSTARIILKHMIPNSLSVIIVYSTLRVPA
                     FIMQESFLSFLGLGVQAPFASWGSLVGDAVDGMTLYPWRLIFPAIAMTLFLFAMNFFG
                     DGLRDAFDPQSKNEL"
BASE COUNT          347 a          482 c          484 g          410 t
ORIGIN      
        1 gcgtgaaccc gctgctccgc cgacggtatt cccacgagct tgagcatttc gatggagcgc
       61 tcgagcgctt cttttttgct gatgcccttc gtatggaggc ggatcgtttc gatcatctgc
      121 gttgaaatcc tcaaaaaggg attcaggctt gtcatcgggt cctggaagat catggaaatc
      181 ttgttacccc ggatcgtgcg catctctttc tcatccaggg cgaggatgtc cgtcccctcg
      241 aagatcgcct ctccgcctac cacctttccc ggaggagagg gaatcagacg catgatcgaa
      301 agattcgtta cactctttcc gcagccgctc tcaccgacga ttcccagagt ttgtcctttg
      361 tgcagggtga aggagacccc gtcgaccgcc ttgacgatgc cggcgtcggt cttgaaatat
      421 gttttgaggt tttgaacgtc taagaccacc tctctatcgt tgactggact catgccatca
      481 tactcctaca gctcattctt tgattgcggg tcgaacgcgt cgcgcaggcc gtctccgaag
      541 aagttcatag cgaacaaaaa gagggtcata gcgatcgcgg ggaaaatcag gcgccacgga
      601 tagagggtca tcccgtcgac cgcgtcgccg acgagcgaac cccacgaagc gaacggggct
      661 tggactccga gacccaggaa ggaaagaaac gactcctgca tgatgaaagc gggaacgcgg
      721 agggtggaat acacgatgat cacgcttagc gagttgggaa tcatatgctt gagaatgatg
      781 cgggcggtcg atccgcccat ggaccgggcg gcttcgacgt actcgctgtt cttcaggctc
      841 atcacctgtc cgcggaccat ccgggcgatc gtcagccagg agacgagggc gagggccccg
      901 aagaggttga ggatgtttcg tccgaaaatc gccatgaaga tgatgacgag gagcatgtac
      961 ggcaggccgt acatgatatc gacgatccgc atcaggatgt aatcgacttt tccgccgaca
     1021 tacccggcga ccgcgccgag gatgatcccg atcagcacac tggtgagcgt cccgaccaat
     1081 ccgatcgcaa tggacacctg actgccgtag atgattcggc tgagcatgtc ccgcccgctg
     1141 tagtcggttc caaggtaata catccgctca tgcttcttcg tcgccgtcac tttcgcctgc
     1201 agctgctcgt tgacgtcgat gatccgctcc acggggaaga ctgcaagccc ctctttgatc
     1261 tcctgcttca aatcacgatc cgcctgacgg gtgatcgtgg tggagatttt tccaaggagg
     1321 ttcgtctttc caagcttggc aatcccgctc tcgccgatcg tctgaagctc cagctccagg
     1381 ttcacctcat attcctcggc gctcagtccg ggggtggcgc tcttgagggt gttcatcagc
     1441 tggctggtca cttcggtaag agtctgagct ttgacggctt cgatatccgt tttcgacaaa
     1501 tcggcgtaga tttgggagat ctcctcatag ctcatcttgc tgaggtcctg ccgtttgccg
     1561 tttttttctt cactgaaggt gatcttggtg acgttgatca ggaactcggt gtcgatcttg
     1621 tcttgaagac gggtgatgac ccgctgatcg gcggcattga aggtgaaggt gcccgcttcc
     1681 cgctgggctt ccccctcttt gaggacgaag ttccaaacct tgt
//
LOCUS       DAOSLI010000138         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_61166_length_1703_cov_25.044903, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000138 DAOSLI010000000
VERSION     DAOSLI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61166_length_1703_cov_25.044903"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLF67_09665"
     CDS             <1..203
                     /locus_tag="PLF67_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOE90316.1"
                     /translation="KQDEILKLISINPLIRAKEVAEQLDLTVDGVWYHLVKMRKSGVV
                     RYERVNGSLPRSTLHDQDVFDG"
     gene            245..622
                     /locus_tag="PLF67_09670"
     CDS             245..622
                     /locus_tag="PLF67_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90317.1"
                     /translation="MSKNKQVKTMETLRKKYEALGEDVSLEKRNLTKAALIRRAYMPT
                     LILEVADFFSFTKEDMLAEYKRVAALDADSAEIKSVVAIKDPLEVKKTHLTMLLYAFE
                     QLSLLRKDDPNAWLLVNELYEDD"
     gene            672..1613
                     /locus_tag="PLF67_09675"
     CDS             672..1613
                     /locus_tag="PLF67_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOE90318.1"
                     /translation="MDWEVRHALNYETLLTAAGGSEAKAGEAFDRAKELLDINEPFDR
                     SYGEYARLLDLDIAAVRQAYEENVRAFGTLEGKVWIINRDSPLWPKQVSMFAYIPRFL
                     YVQGDVNLLKQPIVSVIGTRSPSLEGRKLAYQSSLALAKGNFVVASGLALGIDAIAHK
                     SVLSEGEKTIAVIGTPLNQTYPAEHKELQQIIGEQGAVVSRFGPATRTQKFHFLLRNR
                     LMSALSLANVVVEDRDGGGAVRQASFALEQKRLLFFYQQSLDNHAVLWPRQFLGKERV
                     YVIKKPANLPGAITKAVKGIQNTAKELQKIQQLELFD"
BASE COUNT          413 a          433 c          495 g          362 t
ORIGIN      
        1 tgaaacagga tgagattctc aaactcattt ccatcaatcc cctgatacgg gcaaaagaag
       61 tggcggaaca gctggatctt accgttgatg gcgtgtggta tcatctggta aaaatgagaa
      121 agtccggggt tgtccggtat gagagggtca acgggtcatt gccaaggagt acgttgcatg
      181 accaagacgt gtttgatggt tgacgccaac atggtgaagc gaactatcga ggtacatgtt
      241 cagtatgagt aagaataaac aggttaaaac gatggagaca ctgcgaaaga agtacgaggc
      301 tcttggcgag gatgtcagtc ttgagaaacg caacctcacg aaagccgcgc tgattcgaag
      361 ggcgtatatg ccgacattga tccttgaggt ggccgatttc ttttccttca cgaaagaaga
      421 catgcttgcc gaatacaaac gagtggcggc tcttgatgcc gacagcgccg aaatcaagag
      481 cgtcgttgcg atcaaggatc cgctcgaagt gaagaaaacc catctgacga tgctcctcta
      541 tgcgtttgaa cagctttctt tgcttcgaaa ggacgacccc aatgcctggc tcctggtgaa
      601 tgaactctac gaggacgatt gagcgttttt cccgcattgc cctcttcatc ccggggtgat
      661 acagtatcgg tatggattgg gaagtacgcc atgctctgaa ctatgaaacg ctcctcaccg
      721 ccgccggagg ctcggaggcc aaagcgggtg aggcattcga tcgggcgaaa gagcttctcg
      781 acatcaacga acccttcgat cggagctatg gtgaatacgc ccgattgctg gatcttgaca
      841 tcgccgcggt tcggcaggcg tatgaagaga acgtccgggc gttcggcacg ctggaaggga
      901 aggtctggat catcaaccgt gacagccccc tgtggcccaa gcaggtgagc atgttcgcct
      961 acattccccg gtttctctat gtccaagggg atgtgaacct gctgaagcag ccgatcgtca
     1021 gcgtcatcgg gacgcgctcg ccctcgctgg agggacgaaa gctggcgtat caaagctccc
     1081 tcgctctggc taagggaaac ttcgtcgttg ccagcggctt ggcgctgggg attgatgcga
     1141 tcgcccacaa gagcgtgctc tcggaaggag agaagacgat cgccgtgatc ggaactccgc
     1201 ttaatcagac atatccggcc gagcacaaag agcttcagca gatcatcggc gagcaggggg
     1261 cggtcgtcag ccgcttcgga ccggcgaccc ggacccagaa gttccacttt cttttgcgca
     1321 accggctgat gagcgcctta agtctggcaa acgtcgtggt ggaggaccgg gacggcgggg
     1381 gagcggtccg ccaagcctcc ttcgccttgg aacaaaaacg gcttctcttc ttctatcaac
     1441 aaagcctcga caaccatgca gtcctctggc cccggcagtt tctgggcaaa gaacgggtct
     1501 acgtcatcaa gaagccggcc aaccttcccg gggcgatcac gaaggcggta aaagggattc
     1561 agaacacggc gaaagagctt caaaagatcc agcagcttga actcttcgat tgagccccgg
     1621 aacgtcagga tattgcaatt ggcccttagt ggtgctattg ttctcatacg gttttggggg
     1681 agagaaagtt tttgaccttg gaa
//
LOCUS       DAOSLI010000139         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_66674_length_1603_cov_15.128553, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000139 DAOSLI010000000
VERSION     DAOSLI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66674_length_1603_cov_15.128553"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..770
                     /locus_tag="PLF67_09680"
     CDS             27..770
                     /locus_tag="PLF67_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE90319.1"
                     /translation="MIDALTGGYENNTLFRRISLTFPKGAFIALAGPNGSGKSSLLKF
                     FYKQLKPEEGTIWVEKADLATLKQEVLAQNIGFVPQNGRLDFSFTVEETVAMGRYANP
                     QKDDAKRIYRAMETCSILNLRNTPVTRISGGELQRTFLARALAQDGKILLLDEPVNHL
                     DIAHQRSVMHLLQDLVAKGYTVIAVLHDLLLAQAYSSWIIFLKEGAVAADGPTGEVVN
                     EEILKSVYNVESHRIYDPHLDKELLIPAW"
     gene            853..>1603
                     /locus_tag="PLF67_09685"
     CDS             853..>1603
                     /locus_tag="PLF67_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE90320.1"
                     /translation="MNLAILGAGNIGNKMATTVKQMEGVTLHAVASRSLAKAKEFAKT
                     YSIPKAYGSYEEMLKDRDIELVYVCTPHSHHYEHMKLALEHGKHVLCEKAFTVNAKQA
                     KEILDLGKQKGLLVAEAIWTRYLPMRTVLDQILARRVIGEVQTLTANLAYPVSRIERM
                     WNPELAAGTLLDLSVYTINFAMMVFGNRIETIDGVCTWTNTGVDESNSITLTWAGGKM
                     ATLHASMLGIGDRRGALFGTKGYIEFENINNC"
BASE COUNT          407 a          423 c          424 g          349 t
ORIGIN      
        1 gaggtaccgg tttggctgat gcgatcgtga ttgacgccct gaccggcggg tatgagaaca
       61 atacgttgtt tcggaggatc tccctcacct tccccaaggg tgcgttcatc gccttggccg
      121 gccccaacgg cagcggcaag tcaagtttgt tgaagttttt ctataaacaa ctcaaaccgg
      181 aggaaggaac gatctgggtt gaaaaagcgg atctcgcaac cctcaaacag gaagtcctgg
      241 ctcaaaacat cggattcgtt cctcaaaacg gaaggctgga tttttctttc accgtggaag
      301 agaccgttgc gatgggccga tatgcgaatc cccaaaagga tgatgcgaag cggatttatc
      361 gggcgatgga aacatgttcc attctcaacc tccgaaacac tccggtcacc cggatctccg
      421 gcggggagct gcagcggacc tttctggccc gggcgctcgc tcaggacgga aagattcttc
      481 tgctcgatga gccggtaaac cacttggaca tcgcccacca acgaagtgtg atgcacctgc
      541 ttcaagacct cgtagcaaaa ggatataccg tcatcgccgt tcttcatgac ctgctgttgg
      601 ctcaagccta cagctcctgg atcatcttcc tcaaggaagg agctgtcgcc gcggacggtc
      661 cgaccggtga agtggtgaac gaagaaatcc ttaaatccgt ctacaacgtt gaaagtcacc
      721 gcatctacga tcctcatctg gacaaggagc ttttgatacc cgcctggtag atttttagtg
      781 aaacaaggtg ttcgcatctc cttttcttcg attcttgcga tatactcata ggtatcacaa
      841 ggaggatacg acatgaacct ggcgattctc ggtgccggca acatcggcaa taagatggca
      901 acgacggtga agcagatgga aggagtgacg ctccatgcgg tcgcttcccg atcattggcc
      961 aaagccaagg agtttgccaa aacatactca atccccaagg cgtacgggtc ctatgaagag
     1021 atgctcaagg atcgtgatat cgagctggta tatgtgtgca ctccccactc ccatcactac
     1081 gaacacatga aactggcttt ggaacatggc aaacatgtgc tttgtgagaa agccttcacc
     1141 gtaaacgcaa aacaagccaa ggagatcctt gatcttggaa aacaaaaagg tctgctcgtc
     1201 gccgaagcga tttggaccag atacctgccg atgcggacgg ttttggatca gatcctcgcc
     1261 cgaagggtga tcggggaggt ccaaaccctt accgccaacc tcgcttatcc ggtcagtcgg
     1321 atcgaacgga tgtggaaccc cgagcttgcc gccggaaccc tgctcgacct gtcggtctat
     1381 acgatcaact ttgcgatgat ggtcttcgga aacaggatcg agacgatcga cggagtgtgc
     1441 acgtggacga acaccggtgt ggatgaatca aattccatca ccttgacctg ggcgggcggt
     1501 aaaatggcca cccttcacgc ctcgatgctt ggcatcgggg atcgcagagg agcgctgttc
     1561 ggcacaaaag gatacatcga attcgaaaac atcaacaact gtg
//
LOCUS       DAOSLI010000140         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_67360_length_1592_cov_24.642811, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000140 DAOSLI010000000
VERSION     DAOSLI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67360_length_1592_cov_24.642811"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLF67_09690"
     CDS             <1..699
                     /locus_tag="PLF67_09690"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90321.1"
                     /translation="DQLLDCRREEFFAKEPFYKDLEEEMLTTRSIPNEYLYYYTQRER
                     VLANQRNAPLSRAQTISRLDDELYQTLARGTLDPIRAFNEYMDQRNSSYMMLESGKKR
                     SQPVFSLLDQENANGYDAIALDVLGIASGVLDRQMILNIPNNGFDPALRDEDVIEVSA
                     RRIGGRFRAQGSASALHPSSLKRIRAMKEYERLVIEAVANRSDRAAIQALEANPLILT
                     DPKTLWRKMGGFNE"
     gene            692..>1592
                     /locus_tag="PLF67_09695"
     CDS             692..>1592
                     /locus_tag="PLF67_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOE90322.1"
                     /translation="MSKRSVLVIGPMFADLLVEGASQMPRSGEERYIDSYTVSPGGNA
                     ILALAFARLGLHCALLSTIGDDFIGAELRDSLETEGVDTSTLSVIKSQQSNISLIFHD
                     ASDRSFLTWVQPDPLVHEHASTVARRIESNHFDHVHICFEYLSHRWAQDLLRSLRRSG
                     TSISTGLGYQDSVSWNQGSVEQASLVDWIFMNEEEARRITGKREIRDLLHALREIVRE
                     PVVTLGREGSVALSEKGETIRVPAVDVSVVNTTGSGDSFAAGFIYALLQKRSLMECIR
                     IGGLFGSMTASVKESLSPRIRPEL"
BASE COUNT          340 a          445 c          483 g          324 t
ORIGIN      
        1 gatcagctgc ttgattgccg aagagaggag tttttcgcca aggagccctt ctataaagat
       61 ctggaagagg agatgctcac cacgcgctcg atccccaacg aatatcttta ttactatacg
      121 cagcgggagc gggttctggc gaatcagcga aacgctcccc tcagccgggc gcaaaccatc
      181 agccggttgg acgatgagct gtatcagacg cttgcccggg gtactctcga cccgatccgg
      241 gccttcaacg agtacatgga tcaaagaaac agctcgtaca tgatgctcga gtcgggcaag
      301 aagcgctctc agccggtctt ctcactgctc gatcaggaga atgcgaacgg ctacgatgcg
      361 atcgcgctgg acgtgctggg gatcgcaagc ggagtgctgg acagacagat gatcctgaac
      421 ataccgaaca acggcttcga cccggcgctc cgtgatgagg atgtgatcga ggtctcggcg
      481 aggcggatcg gagggcgctt ccgtgcccaa ggcagtgctt ccgccctgca tccctcctcc
      541 ctgaagcgta tccgggcgat gaaggagtat gaacgtctgg tgatcgaagc ggtggcgaac
      601 aggagcgacc gggcggcgat tcaggcattg gaggcgaacc ccctgatcct gacggatccc
      661 aaaacgcttt ggcggaagat gggaggtttc aatgagtaaa cgatcggttc tcgtcatcgg
      721 tccgatgttt gccgacctgc ttgtcgaagg agcaagccag atgcctcgct ccggcgaaga
      781 gcggtatatt gattcctata cggtttcccc gggagggaat gcgatccttg ccctggcgtt
      841 cgccagactg gggttgcact gcgcgctctt gtcgaccatc ggggacgatt tcatcggtgc
      901 cgagcttcgg gattcgctcg aaacggaagg ggtggatacc tccaccttgt cggtgatcaa
      961 gagccagcaa tcgaatattt cactcatatt ccacgatgct tccgatcgct ccttccttac
     1021 ctgggttcaa cccgacccgc tcgtgcatga gcacgcctcc actgtcgccc ggcggatcga
     1081 atccaaccat ttcgaccatg tgcatatctg tttcgaatac ctgtcgcacc gatgggcgca
     1141 ggatctgctc agaagtctcc gccgatccgg gaccagcatc tcgacgggac tcggctatca
     1201 ggacagcgtt tcctggaatc aggggagcgt cgaacaggca tccctcgtcg attggatctt
     1261 catgaatgaa gaagaagccc ggcggatcac cggaaaacgg gagattcggg atctcctcca
     1321 tgcgttgaga gaaatcgtcc gggagccggt ggtcaccctg ggaagagagg ggtcggtcgc
     1381 gttgagcgag aaaggggaga cgatccgggt tccagcggtc gacgtctccg tggtgaacac
     1441 gacgggaagc ggggattcct ttgcagcagg gttcatctac gcgcttcttc aaaagagatc
     1501 cctgatggag tgcatccgga tcggaggcct gttcgggtcg atgaccgcgt ccgtcaagga
     1561 atccctttcc ccccgaatca gacccgagct cg
//
LOCUS       DAOSLI010000141         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_68841_length_1568_cov_13.187707, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000141 DAOSLI010000000
VERSION     DAOSLI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68841_length_1568_cov_13.187707"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1568
                     /locus_tag="PLF67_09700"
     CDS             30..>1568
                     /locus_tag="PLF67_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90323.1"
                     /translation="MAKHGEAPKAETIENVVNIYKKTGSINETALQAEISPTKVRKIL
                     ITMGLWSSPRSLQIRQLADQGKSSVEIAEMLQVSVVMVQNYLPYKKGLYDEPEKTDTA
                     MRSEKYRARNRSYAQRSRSREHKRPDGLPEDPTTAAPREKKAPYAMLLHLELRDSRLD
                     YFEDDEAKILAKYGGVVKSISRDVIVPSSLALHQLHYLINMAFGWTNSHLHDFKLPQP
                     LFQSLTAGKVLEIAPVFGYYLQFPNSTFDDEFWDDDYEETKSPRTWMRSKYLKQYRYR
                     GISEYWLENQVEIRELAQRRSMLTMHSFGSGAKAEPGQVRFEDVSLQELLNAFMFDSG
                     MPDVLKESLRLSEILSLDPVDIETAKASALAMDSGGIGLYKRYRDVFVAGAKQAKGSR
                     DESIQSHAFLQMQMLQKKYEPKHVMPITDKLLYRYDYGDGWEVDITLVKEFGKDGLMV
                     DDETAAKVIADRRPVCIAKDGLHVLDDCGHVFGYIDMLEIIHGDDREEARDMREWARG
                     QGWTG"
BASE COUNT          402 a          380 c          482 g          304 t
ORIGIN      
        1 cttcagtacg aagaaaggag gaatgggaaa tggcgaagca tggagaagcc ccgaaagcgg
       61 agaccatcga aaacgtagtg aatatctaca agaagacagg ctccatcaat gaaactgcgt
      121 tacaggcgga aatctcgccg acgaaggtga gaaagatcct catcacgatg gggttgtggt
      181 cgagcccgag gtcacttcag atccggcaac tggccgacca ggggaaatcg tcggttgaaa
      241 tcgccgagat gttgcaggtc agcgtggtga tggtccagaa ctaccttccc tacaagaagg
      301 ggctgtatga cgagccggaa aagacggaca cggcgatgag aagcgagaag tatcgggcac
      361 gaaaccgttc gtacgcacaa aggagccgat cccgcgaaca taagagaccg gatgggttgc
      421 cggaagatcc gacgacggcg gcaccgcggg aaaagaaggc tccctatgcc atgctcttgc
      481 atctggagct tcgtgacagc cgtcttgatt actttgagga cgatgaagcg aagatcctcg
      541 cgaaatacgg aggcgttgtt aaatctatct cccgggatgt catcgtcccc tcctcattgg
      601 ctctccatca gctgcattac ctcatcaaca tggctttcgg atggacgaac agccacctac
      661 atgatttcaa gctgccgcaa ccgttgttcc agtcactcac agcggggaag gtgttggaaa
      721 tcgcgccggt gttcggctat tatctgcagt tcccgaacag cacgttcgat gacgaattct
      781 gggatgacga ttatgaggag accaagagcc caagaacctg gatgcgttcc aagtacctca
      841 aacagtacag ataccggggg atcagcgagt attggctcga gaaccaggtc gagatccggg
      901 agctggcaca gcggcgctcc atgctcacca tgcattcgtt cggatcggga gcaaaggcgg
      961 aacccgggca ggtgcggttc gaggatgtat cgttgcagga gcttctgaat gcgttcatgt
     1021 tcgatagcgg catgcccgac gtactgaagg aaagcctccg gttgagcgaa atcctttcac
     1081 tggatcccgt ggacatcgag acggcgaagg ccagcgcctt ggcgatggat tccggcggta
     1141 tcggattgta caagagatat cgtgacgtct ttgttgccgg tgccaaacaa gcgaagggtt
     1201 cgcgtgacga atccatccaa tcccatgcgt tcctgcagat gcagatgctg caaaagaaat
     1261 atgagccgaa gcacgtcatg ccgatcaccg acaagcttct ctataggtac gactatggtg
     1321 atggctggga ggtggacatc acgctggtga aggaattcgg gaaggacggc ctgatggtgg
     1381 atgatgagac ggcggcaaag gtcattgccg accgtagacc ggtctgcatc gccaaggacg
     1441 gtctgcatgt gcttgacgac tgcggacatg tcttcgggta tatcgatatg ctcgagatca
     1501 tccacggaga tgacagagaa gaagcgaggg atatgagaga gtgggcgcga ggtcagggct
     1561 ggaccggc
//
LOCUS       DAOSLI010000142         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_70300_length_1545_cov_26.711409, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000142 DAOSLI010000000
VERSION     DAOSLI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70300_length_1545_cov_26.711409"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PLF67_09705"
     CDS             <1..1121
                     /locus_tag="PLF67_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOE90324.1"
                     /translation="GGRRMTRKEELRIKLDRVRTLMSRLEFDGVFLKRQDDFSWLSCG
                     GQNYIGWGDMGLCGLLVTQDETYAVTNVIEEPRMREEEQLEELGFKIHAGAWQDTGFE
                     ARTLKNLVPSGRIAFDYTHDLGPNIEGEIKKLRLSLTKSEIERMRKVGIMATLALEEA
                     AVSVRPGDTEIEAIKRIANALYDQDMMSTSLMCAADDRLMKYRHAIATPTKIKERFQI
                     GGNMRKWGLTVCLTRYVNFVPVTDELRKQYRLNQLIDTTMMVNTVVGRAYHEPLKAAQ
                     KLYDEHGYGDEFLKHHLGGPIGYANRDYRVDFNLPGTIQENQGFCWNPSITGTKSEDT
                     ILVRKDGFEFITRPIVFPKNEVVVDGKTFIRSDILEKF"
BASE COUNT          416 a          351 c          416 g          362 t
ORIGIN      
        1 acggaggaag aagaatgacg agaaaagaag aattgcgaat caaactggat cgagtaagga
       61 cgctgatgag caggcttgag tttgacggag tgttcctcaa gcggcaagac gacttctcct
      121 ggctctcctg cggcggtcag aactacatcg gctggggaga catgggctta tgcggtctgt
      181 tggtaactca ggatgagacc tatgccgtta ccaacgtcat tgaggaaccg agaatgaggg
      241 aagaggagca gcttgaggag ctgggcttca agatccatgc cggggcatgg caagataccg
      301 gctttgaagc gagaacgtta aagaaccttg tcccctccgg acggatcgca ttcgattata
      361 cccacgatct cgggccgaat atcgaagggg aaatcaaaaa actccgtctt tcgcttacga
      421 aatctgaaat cgaacggatg agaaaagtcg gtatcatggc gacactcgcc cttgaggaag
      481 ccgccgtttc cgtccgtccg ggtgataccg aaattgaagc gataaagcgg atcgccaacg
      541 cgctctatga ccaggacatg atgtccacga gcctgatgtg cgccgccgac gaccggctga
      601 tgaagtaccg ccatgcgatc gccaccccca cgaagattaa ggagcgcttc cagatcggcg
      661 gaaacatgcg caaatggggt ctcaccgtct gtctgacccg gtatgtcaac tttgttcccg
      721 taaccgatga actgcgcaag cagtatcgcc tcaatcagtt gatcgatacg acgatgatgg
      781 tcaacaccgt cgtcggtcgg gcgtatcacg aaccgctgaa agcggcacag aagctgtacg
      841 atgagcatgg atacggggat gagttcctca agcaccacct cggcggaccg atcgggtatg
      901 ccaaccgcga ttatcgggtc gatttcaacc ttccgggaac aatccaggag aatcagggat
      961 tctgctggaa cccctcgatc accggaacga agagcgagga tacgatcctt gttcgaaaag
     1021 acggatttga gtttatcacc cgaccaatcg tcttccccaa gaacgaggtg gtggtcgacg
     1081 gcaaaacctt catccggagc gatattttag agaagttcta actgataata cgcatggatt
     1141 gaatgataat accggtcttc cgtggccggt attttgttcg tccggcatgg tagtaataat
     1201 cggaagaaga atctccccca tggatggagg ttatcctctt tcttcctgat ttgttttgtc
     1261 tcttccgttt ttccggttgc tgcagaaggc gtttggttga tgaaatggaa actaacttgt
     1321 tctaatataa ttagttataa gcatatatcg ggtaatgttt cccagcactt aatcctgaaa
     1381 taagtttgct atcagcaagt aattgatttg taatgaaagt tgacaaaatg aaatgatgtg
     1441 ttagagtaac cctatatgtc cacatggatg gtatgagaag ccatgtgatt cttcttttta
     1501 gagggagggg aagatgaaaa atctgaaacg agtcgacctc tgggt
//
LOCUS       DAOSLI010000143         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_70640_length_1540_cov_11.327273, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000143 DAOSLI010000000
VERSION     DAOSLI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70640_length_1540_cov_11.327273"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLF67_09710"
     CDS             complement(<1..1070)
                     /locus_tag="PLF67_09710"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HOE90325.1"
                     /translation="MNDTDICLVKQNALLKTPAIFKETWPNMKAILIADKNTYPVAGE
                     AVQALFEKHSIEHKVFIFEDEEVIPADYEYVELVKKQLKHNDYVPVAIGSGTINDLVK
                     RAAFEEERTYMVVATAPSVDGYTAYGAAINVDGFKMTLPCDAPKVVIGDAAILTSAPY
                     PMIASGYADLSAKIPAGADWFIADGLKIEKMVPSIFDMVQKDLRDSLANPKLLIEKDE
                     ETILKLFNGLASVGYAMQAYKDSRPASGAEHMVSHIWEMNHLSLNGEPVSHGFKVAIG
                     TLISTAFITELLKLSTEDIKELIEKHKPETWEEREQKIQIAFEDSPALEGALKVNKAK
                     FTKDLTKRHQILIKNWEKIKEKV"
     gene            complement(1107..>1540)
                     /locus_tag="PLF67_09715"
     CDS             complement(1107..>1540)
                     /locus_tag="PLF67_09715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE90326.1"
                     /translation="VEGQGVPEAIDFDGQDGSCSHLLLFDATIPVGTTRIRKTDKGMK
                     LERIAVLPAYRKKGYGELLVKAALSKVTGTIYINAQVASLGFYEHLGFVREDKTTFLE
                     AGIPHTVMLWPHDRGEAPLDTSIFPATVDSEDFDEDSTFLS"
BASE COUNT          295 a          407 c          430 g          408 t
ORIGIN      
        1 accttctctt ttatcttctc ccagttctta atgaggatct gatgacgctt ggtcaaatcc
       61 ttggtgaact tggctttgtt cacctttaag gccccttcca gtgcaggtga gtcctcaaac
      121 gctatttgga tcttctgctc ccgttcttcc caggtttcgg gcttgtgctt ttctatgagt
      181 tctttgatgt cttccgtcga aagcttcaag agctcggtga tgaatgcggt gcttatcaat
      241 gttccgatag ctaccttgaa gccgtgggag accggctctc cgttcaggga aaggtggttc
      301 atctcccaga tgtggctgac catgtgctcc gctccgcttg cagggcggga gtccttgtac
      361 gcctgcattg catagccgac ggacgcaagg ccgttgaaga gcttgaggat cgtctcctca
      421 tctttctcga tcaacagctt agggtttgcc aggctgtctc gaagatcctt ctggaccatg
      481 tcgaagatgc tgggaaccat cttttcaatc ttcagaccgt cagcgatgaa ccagtccgct
      541 ccggcgggga tctttgccga aagatccgca tagccggaag cgatcatcgg atacggggcg
      601 cttgtcagga tggctgcatc tccgatgacg actttcggcg catcgcaggg aagggtcatc
      661 ttgaacccgt ccacgttgat cgctgcaccg tacgcggtgt acccgtcgac cgacggggcg
      721 gtggcgacga ccatgtaggt gcgctcttct tcaaacgcgg cccgcttgac gaggtcgttg
      781 atcgtaccgg atccgatggc gacgggaaca tagtcattgt gtttgagctg tttcttgacg
      841 agctccacgt attcataatc cgccggaatg acttcttcat cttcaaagat gaagaccttg
      901 tgctcgatcg agtgcttctc aaagagcgcc tgaacggctt cacccgctac ggggtaggtg
      961 ttcttgtcgg caatcaggat cgccttcatg ttcggccagg tctctttgaa gatcgccggg
     1021 gtcttcaaca acgcgttttg tttgacaaga cagatatcgg tgtcattcat gcttggttct
     1081 ccatcggttt ggcagaggga tgaagattac gacaagaatg ttgaatcttc atcaaaatcc
     1141 tccgagtcta ctgtagcagg aaatatggaa gtgtccaagg gtgcttcacc ccgatcgtgg
     1201 ggccagagca tgaccgtgtg ggggatgccg gcttcaagaa aggtcgtttt gtcttcccgg
     1261 acgaacccga ggtgttcata gaagccgagg cttgccacct gggcgttgat gtagatcgtt
     1321 ccggtgactt tgctcaaggc cgctttcacc aggagctcgc cgtagccttt cttgcggtag
     1381 gcgggcagga ctgcgatccg ttccagcttc atccctttgt cggtcttccg gatcctggtc
     1441 gttccgacgg gtatggttgc atcaaacagc aggagatggc tacaggaacc gtcctgcccg
     1501 tcgaagtcga tcgcttcggg aacgccctgg ccttcgacga
//
LOCUS       DAOSLI010000144         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_77027_length_1454_cov_21.759114, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000144 DAOSLI010000000
VERSION     DAOSLI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77027_length_1454_cov_21.759114"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLF67_09720"
     CDS             complement(<1..338)
                     /locus_tag="PLF67_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90327.1"
                     /translation="MNTEEARKILDDCLEKHKNLLEQVSALGFIAQGSINERYLTCSS
                     KNCSCHSDPSKRHGPYVYWTTKVQGKTVSRILKGETAELVRQWVANRIELDSIVEQLR
                     QLSVKALEPSV"
     gene            complement(475..>1454)
                     /locus_tag="PLF67_09725"
     CDS             complement(475..>1454)
                     /locus_tag="PLF67_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOE90328.1"
                     /translation="FGQFILTGSAVPAEIDASMHTGTGRISKLYMRTMSLYESKDSRG
                     EVSLGDLFKGGHISAEDDTRLEKIAFLLCRGGWPKAVGLDERPALFQAMDYVDAIVST
                     DISRVDSTRRDKEKAKRLLRSYARTIGTQSSLETIRQDMLANHSDTFDASTLYSYIDA
                     LTKIFVIEDAPAWNPNVRSKTAIRTTDTRYFTDPSIATAALGIGPADLLDDLKTMGFM
                     FENLCIRDLRIYADYLDGNLYHYRDKNGLECDAVIHLRNGSYGLIEIKLGGDSAIEEG
                     AANLKRLASIIDTKKMKAPSFMLVLCARAPYAYKRKDGIHVAAITSLGP"
BASE COUNT          351 a          379 c          335 g          389 t
ORIGIN      
        1 actgaaggct cgagcgcctt gacggatagc tgtcgaagct gttccacgat gctgtccaat
       61 tcaattctgt tggcgaccca ttgacgcacc agttccgcgg tctcaccctt caagatcctt
      121 gagaccgtct tgccttgtac tttggtagtc cagtacacat agggaccatg acgtttggaa
      181 gggtcggaat gacaagagca gttcttggaa gaacacgtca gatatcgttc attgatggag
      241 ccttgggcga tgaagcccaa agcggatact tgttcgagca ggttcttgtg cttctccaag
      301 caatcatcga gtatttttcg tgcttcttcc gtattcatga atcttgcccc catccatacc
      361 ctacagtatt ttgcggtagg gtatacgacc cgtcaagggg ggagatcatg aaagccaacc
      421 ggtggtgcaa taattaaaag aaactgccaa agccattctt caacgagtcc ggtatcacgg
      481 ccccaggctg gtgatggcag ccacatggat accgtctttc ctcttgtagg cgtacggcgc
      541 tcgggcgcac aggacgagca tgaatgaagg agctttcatt tttttcgtat ctataatcga
      601 agccaaccgt ttcaggttcg cggctccttc ttctatcgcg ctgtccccac ccagcttgat
      661 ttctatcagc ccgtaggatc cgtttctcag atgaatgacg gcatcgcatt ccaaaccgtt
      721 tttgtctcga tagtgataca agttaccatc cagataatcc gcatagattc tcagatcgcg
      781 aatgcataga ttctcaaaca tgaaccccat ggttttcaga tcgtcgagaa gatccgccgg
      841 cccgatcccg agagccgccg ttgcaatgga tggatccgtg aaatatcgcg tatccgtcgt
      901 tctgatggct gttttagatc gcacgttcgg attccaagcc ggagcatctt cgatgacgaa
      961 aatcttagta agggcatcta tatatgaata cagggtcgag gcatcgaatg tatccgaatg
     1021 gttggccagc atatcctgcc tgatcgtctc caaggaactt tgagtcccta tagttcgtgc
     1081 ataagaccga agaagtcttt tcgccttttc cttgtccctt cttgtagaat ccactctgct
     1141 gatgtcggtc gaaacaatgg catcaacata atccatcgct tgaaaaagtg ccggtctttc
     1201 gtctaaaccc acagccttcg gccaacctcc tctacagagt aaaaaggcta ttttttccaa
     1261 ccttgtatcg tcttctgcgg aaatatgccc ccctttgaac aaatctccaa gagaaacctc
     1321 tccgcgtgaa tctttggact cgtagagact catagttcgc atgtacaact ttgaaatcct
     1381 acccgttccg gtgtgcatgg acgcatcaat ttcagcggga acggcagatc ccgtcaggat
     1441 gaattggccg aact
//
LOCUS       DAOSLI010000145         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_78976_length_1430_cov_20.821818, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000145 DAOSLI010000000
VERSION     DAOSLI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78976_length_1430_cov_20.821818"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>1430)
                     /locus_tag="PLF67_09730"
     CDS             complement(81..>1430)
                     /locus_tag="PLF67_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOE90329.1"
                     /translation="QSRKGKLPFKIVGFVDDNPDYDRSSILGIRVYGTIKELTQILKE
                     ARAKVLIIAITHIEQEKMLEAVDAAREADCEIKVIPSLFEMQDKPLEEVDVRSLDYAD
                     LLGRPLIKIDREPIRQMVQGKTVLVTGAGGSIGQEICRQLLSYGPVKLLLFDIDETEL
                     HDLSLRLHHYQREWSDWIYPIVGDIRNKQKVNTVFEIYQPQLVFHAAAYKHVPLQELY
                     PEEAVQTNILGSYNVLKAAKDHKAEKVVVISTDKAVNPTNVMGATKRVVELLARMLTS
                     DETEMVSVRFGNVLGSRGSMLPLFVEQIKAGVPVTVTHKDIIRYFMAIPEAVGLVFKA
                     ASMAKGGEVMVLDMGQPVKIYDFAKKLVQYYGDENSKIIITGLRPGEKLYEELLADKD
                     TTMPTDDPLVFKAKVNNNIFSEETFLNEHYDVFASGEKEELLKHLHELVPEFRMHQH"
BASE COUNT          315 a          399 c          361 g          355 t
ORIGIN      
        1 tgatcgttgg atggcgaacc aagctcaaca atcacctctc tgccttccgg gatgcagaga
       61 tgatccagtg tagcacttta tcagtgctga tgcatcctga actcgggaac gagttcatgg
      121 aggtgcttca acagctcctc cttctctccg gatgcaaaga catcataatg ctcgttgagg
      181 aacgtctctt cactgaagat gttgttgttc accttcgctt tgaagaccaa cggatcatcc
      241 gtcggcatgg tcgtgtcctt gtctgcaagc agttcctcat agagcttctc accgggacgg
      301 agcccggtga tgatgatctt gctgttttcg tctccatagt attggacaag cttcttggca
      361 aagtcataga tctttaccgg ctggcccata tccaacacca tcacctcccc gcctttggcc
      421 atcgaagccg ccttgaacac gaggcccacc gcctcgggaa tcgccataaa gtaccggatg
      481 atatccttgt gggtgaccgt gacgggaacc cccgccttga tctgctcgac gaagagcggc
      541 agcatcgaac cgcgggaacc caggacattc ccgaacctca cggataccat ctcggtctca
      601 tcgcttgtca gcatccgggc aagcagctcg acgacccgct tggtcgcccc catcacgttg
      661 gtgggattca ccgccttgtc ggtggagatg actaccactt tttcagcctt atggtctttg
      721 gcggctttca ggacattgta ggaaccgagg atattggttt gaaccgcttc ttcggggtac
      781 agctcctgaa gcggcacatg cttgtacgcc gcggcatgga acacaagctg cggctgatag
      841 atttcaaaga ccgtattgac tttctgtttg tttcggatgt ccccgacaat tggatagatc
      901 caatcagacc attcccgctg atagtggtgt aatcgaagag acagatcatg cagttccgtt
      961 tcatcgatat cgaaaagaag gagcttgacc ggaccgtagg aaagcagctg ccggcagatc
     1021 tcctgaccga tcgagccccc ggctccggtg accagaaccg tcttgccctg aaccatctgc
     1081 ctgatcggtt cacgatcgat tttgatcaat ggtctgccga gaaggtcggc ataatcgagg
     1141 gagcggacat ccacttcctc aaggggcttg tcctgcatct cgaagagcga aggaatgacc
     1201 tttatctcac agtcggcctc tctcgccgca tcgaccgcct caagcatctt ctcctgttca
     1261 atatgggtga tcgcgatgat caggactttg gcccgggctt ctttaaggat ctgcgtcaat
     1321 tctttaatcg ttccatagac ccgaatacca agaatggaag aacggtcata gtccgggttg
     1381 tcatcgacga agccgacgat cttgaaggga agcttccctt ttctgctttg
//
LOCUS       DAOSLI010000146         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_80979_length_1405_cov_22.241481, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000146 DAOSLI010000000
VERSION     DAOSLI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80979_length_1405_cov_22.241481"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLF67_09735"
     CDS             complement(<1..439)
                     /locus_tag="PLF67_09735"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HOE90330.1"
                     /translation="MAQDLRIITTGGTFDKQYDAIKGELTFRESQLPRILLQARCTLT
                     VHLEGPLAVDSLYMTEQQREEIAQACVHSVEDRIIIIHGTDTMCATARVIAKSNIDKT
                     IVLTGAMTPYSLEGSDAVFNLGSAISAVQLLEPGVYITMSGRIL"
     gene            complement(396..734)
                     /locus_tag="PLF67_09740"
     CDS             complement(396..734)
                     /locus_tag="PLF67_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90331.1"
                     /translation="MEKLFSLKGEGLDGETHIRIDDYGTTFVDKSYHDENILAEDVSV
                     MPVELSVTVLLIQPGGLFDEYFLLFVLIGPNHIFASAKLFFILALEVILSSGGSYGTG
                     FEDYYDRRDV"
     gene            684..>1405
                     /locus_tag="PLF67_09745"
     CDS             684..>1405
                     /locus_tag="PLF67_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HOE90332.1"
                     /translation="MGLTVESFTFQGEELLHQDSARKRAGRTYGIPILFPTPNRVAND
                     RYVFNDKMVPAVMHGFLRHRGFGIESRSDTHIAGRITFDGSEPLFPYNAEVMVTISLI
                     GSTLRWEFHLTNTGDEPFGYGLALHPFFVKKKGMTLETNGHQRLVTDENMLPTEEIVE
                     ERPSSDVDTLASDAIFRCDGPSTSTLRGDGIELKILGSPAFGYTVVYTGLESAFICIE
                     PQSCAINAHNLADQELAALQVL"
BASE COUNT          349 a          348 c          362 g          346 t
ORIGIN      
        1 gcaggattcg tccgctcatc gtgatatata cccccggttc caacagttga accgcgctga
       61 tcgccgaacc gagattgaag acagcgtccg acccctccag agaataggga gtcatcgctc
      121 cggtgaggac gatggtttta tcgatattgc tcttggcgat gacccgggcg gtcgcgcaca
      181 tcgtgtcggt tccatggatg atgatgattc gatcttccac actatgaacg cacgcttggg
      241 cgatttcttc tcgttgttgc tcggtcatgt acaggctgtc gaccgccagc ggcccttcaa
      301 ggtgtacggt caacgtgcat cgggcttgca aaagaattcg aggcagctga ctctcccgaa
      361 acgtcaattc acccttgatc gcatcatact gcttgtcaaa cgtccctccg gtcgtaataa
      421 tcctcaaatc ctgtgccata tgatccccct gaagacagta tcacctcaag agcgagaata
      481 aaaaagagtt ttgccgatgc aaaaatgtgg ttgggtccta tcagaacgaa aagaaggaaa
      541 tactcgtcaa agagtccccc gggttggatg aggagtacgg taacggaaag ttccactggc
      601 ataacggaca catcttccgc taagatattc tcatcatgat aactcttgtc aacaaatgtt
      661 gtaccgtagt cgtcgatccg gatatgggtc tcaccgtcga gtccttcacc tttcagggag
      721 aagagcttct ccatcaggat tccgctcgga aaagagccgg aagaacgtat ggaatcccca
      781 tcctcttccc gacacccaat cgggtagcga atgatagata tgtgttcaac gataaaatgg
      841 ttccggccgt gatgcacggg tttctgaggc atcggggttt tggcattgaa tcaagaagtg
      901 atacacacat tgcaggaagg atcacctttg atggaagcga gcctctcttt ccttacaacg
      961 ctgaagttat ggtaacgatt tccctgatag gttcaactct gcgctgggag tttcacttga
     1021 cgaataccgg tgacgagccc tttggctatg gattggccct tcatcccttc tttgtgaaga
     1081 agaaagggat gacgttggaa acgaacggtc atcagcgcct tgtcaccgat gaaaacatgc
     1141 ttcccaccga agagatcgtt gaagaaagac cttccagtga tgtggatacc ttggctagcg
     1201 atgcaatctt tcgttgtgac ggcccatcga cgagtaccct tcgcggagac ggaattgaat
     1261 tgaagattct aggcagtccg gcgtttggct atacggtggt gtatacggga ttggagtcgg
     1321 cgttcatctg tatagaacct caaagctgcg ccatcaatgc gcacaatctt gcagatcagg
     1381 agctggcagc cttgcaggtt ctgga
//
LOCUS       DAOSLI010000147         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_81207_length_1402_cov_29.697847, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000147 DAOSLI010000000
VERSION     DAOSLI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81207_length_1402_cov_29.697847"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1349)
                     /locus_tag="PLF67_09750"
     CDS             complement(57..1349)
                     /locus_tag="PLF67_09750"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOE90333.1"
                     /translation="MAVTTGGSGSWSVINSLLEGIPIPKMVKVHQNFFLPEVIDIQNA
                     LAAQLSSKTLWAPLKRGDTVAIGIGSRGIDGQIEAVRTLVSAIKERGGVPFLVPAMGS
                     HAGATAEGQKAMLLTLGMDQVDAPIKSSMETVLLGRCPDGMPVLVDRFAHEADHLIII
                     NRIKSHVCFRGPYESGLVKMIAIGLGKQQGAEIAHNLGFGRMPRHIEAIATEALNHLK
                     LLFAVALVEDATHRCSHIEVIAPDDLLTREPELLKLSKSWEARLHFETLDALVIDEIG
                     KHISGSGFDTNVVGRYHSEHVTGGPKITRVAILDIASESNGNGNGLGMADFTTVRAVE
                     KFDFAQTYPNTLTALLTGGVKIPMVLMNDRQVFQACMKTSYRQRWEEARIVRIRNTLS
                     LGEIEVSESIVPEIEHDSCFEIIGEPYELQFNDEGNLF"
BASE COUNT          299 a          417 c          351 g          335 t
ORIGIN      
        1 actcgatcca gtttgattcg caattcttct tttctcgtca ttcttcttcc tccgtattaa
       61 aatagatttc cttcatcatt gaattgaagc tcgtatggct cgccgataat ctcaaagcag
      121 ctgtcatgtt caatctccgg tacgatcgat tccgatacct cgatctcgcc caaggaaagc
      181 gtgttccgga ttcgaacgat ccgggcttct tcccatcgct ggcgataact tgtcttcatg
      241 catgcttgaa acacctgccg atcattcatc aggaccatcg ggatcttcac ccctccggtt
      301 agcaaggcgg taagcgtgtt cgggtaggtc tgagcgaagt cgaacttctc caccgcccgg
      361 acggtcgtaa aatccgccat tcccaagccg ttgccgtttc cgttggactc ggaagcgata
      421 tccaaaattg ccacccgggt gatcttcggt ccgccggtga catgctccga atgatagcgg
      481 cccacgacat tcgtatcgaa accgctgccg ctgatgtgtt tgccgatctc atcgatgacc
      541 aaggcatcaa gcgtctcgaa gtgaagccgg gcttcccagc ttttcgaaag cttgaggagt
      601 tccggctccc ttgttaagag atcatcgggg gcgatcacct cgatgtggga acagcggtgg
      661 gttgcatcct cgacgagggc gacggcgaag aggagcttca gatggttcag cgcctcggtc
      721 gcgatcgctt cgatgtggcg aggcatccgc ccgaatccga gattgtgggc gatctccgct
      781 ccctgctgct ttcccagacc gatcgcgatc atcttgacca gaccgctttc atacggcccg
      841 cggaagcata catggctctt gatgcggttg atgatgatca gatgatccgc ctcgtgggca
      901 aaacggtcga caagaaccgg cattccgtcc gggcacctgc cgagcaacac cgtctccatc
      961 gaacttttaa tcggtgcatc gacctgatcc atgccaagcg tcagaagcat cgctttttga
     1021 ccttcggcgg tggccccggc atgactgccc atcgccggaa cgagaaacgg aactccaccg
     1081 cgctctttta tcgcggaaac gagcgttcgc acagcctcga tctgtccgtc gataccgcgg
     1141 cttccgatcc cgatcgctac cgtatcaccc ctcttaagcg gtgcccaaag cgtttttgaa
     1201 gagagctgag cggcaagcgc attctgaata tcgattacct ccggaaggaa gaagttctgg
     1261 tgcaccttca ccattttggg gatgggaatc ccttcaagca gtgaattaat gacgctccac
     1321 gaaccgcttc caccggtggt aaccgccatc cctacgcctc cttctttttc acgttataca
     1381 atgcaagccc aatagaaaga gc
//
LOCUS       DAOSLI010000148         1401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_81303_length_1401_cov_24.069837, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000148 DAOSLI010000000
VERSION     DAOSLI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81303_length_1401_cov_24.069837"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..>1401
                     /gene="ugpC"
                     /locus_tag="PLF67_09755"
     CDS             322..>1401
                     /gene="ugpC"
                     /locus_tag="PLF67_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOE90334.1"
                     /translation="MATVTLKNISKVYDGGVKAVDNVNIEIKDREFVVLVGPSGCGKS
                     TTLRMVAGLEDITSGELYIDDKLVNDVPPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     IRKFPKDEIDQRVREAAKILEIEELLDRKPKALSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRAEISSLHHRLQATMIYVTHDQVEALTMADVIVVMKFGVIQQIGG
                     PLELYNHPANKFVAGFIGSPPMNFLVTQIEADGDAIYANEGTFRLKVTAEQKKYLTPY
                     VGKSVTFGIRPEDVEFHHTKKDGETIDGVVSVVEPLGSETHVYITTSKSQVIGKIEPN
                     LVPAPDTKISLIPNMAKAKFFDLETE"
BASE COUNT          406 a          352 c          361 g          282 t
ORIGIN      
        1 cggaaagaaa tgcaagaatt tatggggctg accggtagaa gaaacttttc ggaaaaatat
       61 ataaagccgt tgcttaatgc aggggaaatt gaaatgacga tcccggataa gccgaacagt
      121 ccaaatcaaa ggtacaggaa aaaacagctt gatcgttaac caatcaggac aagagaccaa
      181 gtttaatgga gtgtgcgtgc atttcaaggc gccaatagta atggtgcttt gaaaagcgca
      241 aatcatcttc acccatcaaa ggtttgacac cggagcttga gtggggtaca gtcacagaag
      301 ataaaataat gaggaggcat tatggccaca gtaacactga aaaacatctc aaaggtgtat
      361 gacggtggag tcaaagcagt tgacaacgtc aacatcgaaa tcaaggaccg ggaattcgtc
      421 gttctcgtcg gcccatccgg atgtggaaag tccacaaccc tcagaatggt cgccggtctt
      481 gaagacatca cttccggcga gctctacatc gatgacaagc tcgtcaatga cgttcccccg
      541 aaagatcgtg atatcgcgat ggttttccag aactatgcgc tctatccgca catgaccgtc
      601 tatgacaaca tggcgttcgg tctgaagatt cgaaagttcc ccaaagacga aatcgaccag
      661 agggtacgcg aagctgctaa aattcttgaa atcgaagagt tgctcgatcg aaaaccgaaa
      721 gcgctctccg gtggacagcg ccagcgcgtt gcggtaggac gggcaatcgt tcgaaagccg
      781 aaggtattcc tctttgacga accgctttcc aaccttgacg caaagcttcg agttcagatg
      841 cgcgccgaga tctcttcgtt gcaccacaga ctgcaggcaa cgatgatcta cgttacccac
      901 gaccaggtcg aggccttgac gatggcggac gtgatcgtag tcatgaagtt cggtgtcatc
      961 caacagatcg gcggaccgct cgagttgtat aatcacccgg cgaacaagtt cgttgccggc
     1021 ttcatcggaa gccccccgat gaacttcctc gtaacgcaga tcgaagcgga cggcgatgcc
     1081 atctatgcaa atgagggaac cttccgcctg aaggtaaccg ccgagcagaa gaagtatctc
     1141 accccgtatg tcggcaagag cgtcaccttc ggtatccgcc ccgaagacgt cgagttccac
     1201 cacaccaaga aggacggtga gacgattgac ggcgtcgtaa gcgttgtcga accgctggga
     1261 agcgagacgc atgtctatat cacgacaagc aagagccaag tcatcggtaa gatcgagccg
     1321 aacctcgtac ccgcaccgga tacgaagatc tccttgattc ccaatatggc gaaagccaag
     1381 ttcttcgatc tcgaaacgga a
//
LOCUS       DAOSLI010000149         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_81612_length_1397_cov_22.712370, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000149 DAOSLI010000000
VERSION     DAOSLI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81612_length_1397_cov_22.712370"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLF67_09760"
     CDS             complement(<1..890)
                     /locus_tag="PLF67_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE90335.1"
                     /translation="MKLLEESIEIDYDELMTEYDLPRRMKRLLKLAQRVSAWDPDELT
                     PDRIAELNGKAHPSSLLIRRLFGKPARNITTKTFVIPVNDGKVTGYLFEKEDSRGISD
                     LLPLIIYYHGGGWVWGNMDLYNIICAHLADVTGATVLSVDYRLAPQHRFPTAVEDCYN
                     TLLWAAAGCRYWKNDPDRIFLVGDSAGGNLAAVVSRLARDRKGPAIEGQVLIYPITDG
                     RMRTASYAEHKDSPTLTDRQMAFYIHNYQREPKDTLDPNFSPLLAKDHSRLPPTLIIG
                     AEYDPLRDDGVLYADALASA"
     gene            932..>1397
                     /locus_tag="PLF67_09765"
     CDS             932..>1397
                     /locus_tag="PLF67_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOE90336.1"
                     /translation="MSTHTRFGYDFIKEPFLPEGKDEYYLRFAQNGKDPGTYRNLRSD
                     EIAILKSNLNHSPNWDNLLVRDPFDPLLIRNSEFYGLVRLGTVSVGTVSFHDFTVPVG
                     IVNSRIVSCDIGDSCSIMECTYLSHYIIDDDVVLYRIGEMQSTNHAKFGNGIL"
BASE COUNT          307 a          370 c          415 g          305 t
ORIGIN      
        1 tccgccgaag cgagcgcatc ggcatacaga acgccgtcat cccgaagcgg atcgtactcc
       61 gccccgatga tcagggtggg gggaaggcgg ctgtggtcct tggcgagcag cggagagaag
      121 ttcggatcga gggtatcctt cggttcgcgt tggtagttgt ggatatagaa cgccatctgc
      181 cgatcggtca aggtggggga gtccttgtgc tcggcgtagc tggcggttcg catccggccg
      241 tcggtgatcg gatagatcag aacctgccct tcgatcgcag ggcccttgcg atcccgcgct
      301 agacggctca ccaccgcggc aaggtttcct ccggcgctgt cgccgacgag gaagatccgg
      361 tcggggtcgt tcttccaata gcggcagccg gcggcggccc acagaagggt gttgtagcaa
      421 tcctcgacgg cggtcggaaa acggtgttga ggcgcaagac ggtagtcgac ggaaagaacc
      481 gtggccccgg taacatccgc cagatgggcg cagatgatat tgtaaagatc catgttcccc
      541 cacacccagc cgccaccgtg gtagtagatg atcaggggca gcagatcgct gattccccgc
      601 gagtcctctt tttcaaagag gtagccggtg acttttccat cgttgacggg aatcacgaac
      661 gtcttggtgg tgatgtttcg agccggcttt ccgaagagcc ggcggatcaa cagggaggag
      721 ggatgggctt tgccgtttaa ctcggctatc cgatcagggg tcagctcatc gggatcccaa
      781 gcggaaaccc gttgcgccaa cttcagcaat cgcttcatcc gccgaggcaa gtcatactca
      841 gtcatcaatt catcgtagtc tatctcaatg gactcttcaa gaagcttcat cggttgtatt
      901 ggaaggaaag aggggagtat actgtttcca tatgagcaca catactcgat ttggttatga
      961 tttcatcaaa gaaccgttcc tgcccgaggg caaggatgag tactatctcc gctttgcgca
     1021 gaacgggaaa gacccgggca cataccgaaa tctgagaagc gatgagatcg caattctcaa
     1081 gagcaatctg aaccacagtc cgaactggga caatcttttg gtcagagatc cctttgatcc
     1141 gctgttgatc cgaaacagtg agttctacgg cctcgtccgg ctcggtacgg tatcggtggg
     1201 tacggtgagt tttcacgatt tcaccgttcc cgtcggcatc gtgaactccc gaatcgtcag
     1261 ctgtgacatc ggagattcct gctcgatcat ggagtgcacc tacttaagcc attacatcat
     1321 cgacgatgac gttgtgctct atcggatcgg ggagatgcag agcaccaacc acgcgaagtt
     1381 cggcaacggg attctga
//
LOCUS       DAOSLI010000150         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sphaerochaeta sp. isolate AMR_MDS_1627
            NODE_107187_length_1162_cov_27.106594, whole genome shotgun
            sequence.
ACCESSION   DAOSLI010000150 DAOSLI010000000
VERSION     DAOSLI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863156
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphaerochaeta sp. (wastewater metagenome)
  ORGANISM  Sphaerochaeta sp.
            Bacteria; Spirochaetota; Spirochaetia; Spirochaetales;
            Sphaerochaetaceae; Sphaerochaeta.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:40:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,915
            Genes (coding)                    :: 1,914
            CDSs (with protein)               :: 1,914
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Sphaerochaeta sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107187_length_1162_cov_27.106594"
                     /isolate="AMR_MDS_1627"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1972642"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLF67_09770"
     CDS             <1..1134
                     /locus_tag="PLF67_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE90337.1"
                     /translation="PLIASYDLGMLARLTGDWKHSNELLSDAERRIQDAYTKSITASL
                     ASFVVNDNTKEYGGESYEDIYLNLFKALNYLHLGQYESSLVEIRRMIEKQSVLQDRYE
                     QQANQARRYASTNHIRQTDLPAQATSFTTSALANYLGVIIAAHLGEQSTLDYSFNQIH
                     HAFETQTKLYPFPIPESVEPLKREGRDSVHLIAFTGRFPVKEERMDFISISPTNVTRI
                     AYPVLRPIPSAITSIRVRVDGKVITTLEKIESFSLVAEDAFQSKSSLLYAKATMRAIS
                     KELGIGIWDAISKDKDGKRSAFAEVLGFAFRVGKELSESADLRSTHYLPAEAWVGTLD
                     LEPGSYTIEVEYLNRIGQVLHTERFMETIDPTTINLLESYSPL"
BASE COUNT          277 a          357 c          287 g          241 t
ORIGIN      
        1 cccctgatcg cgagctatga cctggggatg cttgcccggc tcaccgggga ttggaagcat
       61 tccaatgaac tgctcagcga tgcggagagg cgaatccaag atgcgtatac gaaatcgatc
      121 actgcatcac ttgcctcctt cgttgtcaat gacaacacca aagagtatgg cggtgaaagc
      181 tatgaggata tctatctcaa cctcttcaaa gcgctgaact atctccatct cggccagtat
      241 gagtcgagtt tggtcgaaat ccggcggatg atcgaaaaac agagcgtatt gcaagatcgt
      301 tatgaacaac aagcgaacca agcgcgccgt tatgcgtcaa ccaaccatat ccgccaaacc
      361 gatctgcccg cccaggccac ctcgttcacc accagtgcgc tcgccaacta cctcggggtg
      421 atcatcgccg cccatctcgg tgaacagtcc accctcgatt actctttcaa tcagattcac
      481 catgcgttcg aaacccagac gaagctctac cccttcccca ttcccgaaag tgtcgagccg
      541 ctcaagaggg aaggtcggga ttccgtccac ctgatcgcct tcaccggacg ctttcccgtc
      601 aaggaggagc ggatggattt catcagtatc tcccccacca acgtcacccg aatcgcctac
      661 ccggttctcc ggccgattcc ttcggcgatc acttcaatcc gagtccgggt tgatggaaag
      721 gtcattacca ccttggaaaa aatagaatca ttctccctcg tcgccgagga tgcgtttcaa
      781 tccaaaagca gcctgctcta cgccaaagcg accatgcggg cgatctccaa agagctaggc
      841 atcggcatct gggacgccat ttcgaaagac aaggacggca agcggagcgc cttcgccgag
      901 gtcctcggct ttgcattccg agtgggcaaa gagctgagcg aatcggcgga tcttcgttcg
      961 acgcactacc ttccggcaga agcctgggtg gggacgctcg atctcgaacc gggttcctac
     1021 acgatcgagg tggagtacct caatcgaatc ggacaggtgc ttcacaccga gcgcttcatg
     1081 gagacgatcg atcccacgac gatcaacctg cttgaatcgt acagtcccct ctaggcatcg
     1141 agggcgacca tctcttcctg tt
//
