LOCUS       DAOSLP010000001        69937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_450_length_69937_cov_11.450703, whole genome shotgun sequence.
ACCESSION   DAOSLP010000001 DAOSLP010000000
VERSION     DAOSLP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 69937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69937
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_69937_cov_11.450703"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..736)
                     /gene="araD"
                     /locus_tag="PK460_00005"
     CDS             complement(47..736)
                     /gene="araD"
                     /locus_tag="PK460_00005"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HOE53519.1"
                     /translation="MYKTLKETTYNANIAIKKSGLVLLTWGNVSVKDDVLKVIAIKPS
                     GVAYEKMKADDIVVVDFDGNIIEGTLRPSVDLPSHLALYKAHQEIGAIVHTHSTFATA
                     WAQKGRAIPMYGTTHADYFSDDIPCAPYLTPEEMKDYEKNTGEVIAALIKKGRANETP
                     AAIIKGHGAFAWGKNAKDAVLHAIVLEEVAKIAYLTEAIPGQNNPLPEHIKKTHYERK
                     HGPNARYGQKK"
     gene            complement(740..2176)
                     /gene="araA"
                     /locus_tag="PK460_00010"
     CDS             complement(740..2176)
                     /gene="araA"
                     /locus_tag="PK460_00010"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOE53520.1"
                     /translation="MKTFWFIVGSQSLYGEETLKQVEKNAKKIAKGLKTKYPIVYKET
                     VKTHAEAMRLIKDANYDENCLGIITFCHTFSPSKMWVNALQNLQKPWCHFHTQFYDEI
                     PNDAIDMDYMNLHQSAHGDREHGFIGTRLQKNRKIIVGYWQDPKTSEAISAWQDVVDA
                     VNESKTLSVVRFGDNMREVAVTEGDKVEAQMKFGWQVNTIGVGTLVDTIKAVTNEELT
                     AKLAEYKAKYALNTDNFASIEYQAKAEVAIKKILDANNAKAFTNTFEDLYNMEQLPGL
                     ATQNLMLEGYGYGAEGDWKTAAMTRIFKAIGKVTTFMEDYTYDWKEGLILGAHMLEVC
                     PSIARERPKIEVHHLGIGGKKAPARLVFEGKPGKAKAISLIDLGHRFRLIALDVECVA
                     PTKSMPNLPVARLMWRPEPNLEKSAACWLVAGGAHHTTLVYDLSLEFLRDFANLLDIE
                     FVHIGKDADPYILEEMMKNNDIRYRLRG"
     gene            complement(2180..3484)
                     /locus_tag="PK460_00015"
     CDS             complement(2180..3484)
                     /locus_tag="PK460_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOE53521.1"
                     /translation="MYLGIEFGSTRIKGIVLNDNFEVIASGSYIWQSTLKDGYWTYDL
                     NEVWVGMNAVIDEIAKTINVKEIKSLGVSAMMHGFLPFDKNGKLLTPFRTWKNTNTTV
                     AATELSKQFAFNIPLRWSIAHLYQAYLDKESYVKDIDTFTTLAGYVHLTLTGKNVLGI
                     GDASGVFPIDVKTKHYNQKMIQTFNDLTDMKLEKLLPGIVEVGEDAGFLTAKGAELLG
                     NKLTVGIPLAPPEGDAETGMVSTNALKPKTGNVSAGTSVFSMIVLEKALNKWYPEIDI
                     VQTPTGDAVAMVHANECTSRIDPWIELFGEAIMRVNGEVDRNHLFETLYEVSLEDTPL
                     GEFMKQKLVEAIIDLKPGMDILLNKEHVKVDYLLGHGGYFKSKSAGRKVMSETLGIPI
                     RLLETAGEGGPWGMAVLAAYRYYNKEQKIKFDKFIEKVFKGA"
     gene            complement(3497..5125)
                     /locus_tag="PK460_00020"
     CDS             complement(3497..5125)
                     /locus_tag="PK460_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOE53522.1"
                     /translation="MKRYLFLISVAILALTACSGGKTITSISNSDNIEEAIIYPTKPY
                     SDSDHIMQKDPYAFYNIAGNDTRYWGAANTHDPAIIKEGDTYYAFSTDANFGVTSQKG
                     IHIRKSNDLIKWSFVGTALDVTTLEDQIEYIGYNRDGEKVDFFWAPDIIKRGNEFWLF
                     YSISGFGQRNSMMGLAKSNNIEGPYTFDKEILRTSQSVGGTPNAIDSAIFVEEVDGEE
                     KMYMSYGSWSAGIYIIELDPVTGYPLIAQTLVEREVTVNTAEKGVTATATKLVPSSSD
                     DPAFGTKILSITSSEAPYIIKENGYYYLFITNGVDLTYDYDVRVFRSRNILGPYVDGK
                     GVSALSATNYNSFRPYGNKVTIAHQFTYLTDDISYKRGWAGIGHSTAFKDEEQWFFAS
                     HYRGTYMDRHRFFLGIWKMHFVNDWPVIEPNRYTGEGSINLEQADISGNYRIQILHQS
                     AVNSMLTDDIISIVNRAEEITLTKQKAGEWYVVNGEYNGRYRLNNNQLELELNGVLYK
                     GVITPQFNYEFEKGVLGLSLINSDGTALWGNRII"
     gene            complement(5138..6616)
                     /locus_tag="PK460_00025"
     CDS             complement(5138..6616)
                     /locus_tag="PK460_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOE53523.1"
                     /translation="MKTKLFIDKNLTIGKVSNLLFGSFIEHLGRAVYTGIYEPGHPKA
                     DKDGFRQDVIDLIKELKVPMVRYPGGNFLSGYNWEDGIGPKENRPVRLDYAWQTIEDN
                     TFGTDEFMRWCKKAEIEPMMAVNLGTGTPKDAGNIVEYCNFPKGTYYSDLRIKNGHKE
                     PYNIKYWCLGNEMDGPWQICYLNAEDYAKKALETAKIMRWVDPSIKLIACGSSNAEIE
                     TYPDWDRIVMETLYDHVDYISMHRYYWYEDSLEDFFASYHDMDNFIHTIKSTADFVKA
                     KKRSDKVMKISFDEWNVWYLNKWKPGGWKKAPHILEDHYSLKDALVFSGLLNTLVNNV
                     DRVEIAALAQLVNVIAPIFTKEGGGVFKQTIFYPFYAVSNYGRGVALKAIKTGPKFNS
                     KFGEVNYVSEAVTFNEEKREISVFLVNYAQEKMEVELELRSFGRVEALTHKVMTHDDL
                     EIKNTFDEENVKFVEGELPTVKNDVATLELGPLSYHVYRFKY"
     gene            complement(6631..7485)
                     /locus_tag="PK460_00030"
     CDS             complement(6631..7485)
                     /locus_tag="PK460_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE53524.1"
                     /translation="MKKSNIKANRSIKRAKLFSDISTYVILVLGSVLMLAPLIWMLSA
                     SLKVNQFSVYGPLFHEWNFSNYIEVFKTIPVWKGLLNSVIVAVPSIIVGTFVSGLAAY
                     AFAKIKFKFCNIIFILMLAVMMIPFPVIMIPQYYIFSDLDWIGTLLPLIIPKLFGNIM
                     MIFFLRQFLTHIPDALVESAKIDGANHFQIFWIIILPLLKPALAAQTVLWFIGIWNDY
                     LAPNFFSAASPTLPVVIGGLVSDFPIQEPVHLNMAASIISMIPVLAIFAIFQKQIINS
                     FVLSGMKE"
     gene            complement(7482..8390)
                     /locus_tag="PK460_00035"
     CDS             complement(7482..8390)
                     /locus_tag="PK460_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE53525.1"
                     /translation="MNTNIVEKPKKRKVKHVQENIIGYLFILPVFIGIAFFIVYPFFY
                     SLVSTFKNWNGLVPLSVAPTVGFEMYRRVLSDSLFWQAFGNTLITLIGIPIGMILSIA
                     IAMLLNRGLKFTGIFRVIFYIPVVSSITAIIILWRAMMNTQGPINDFLALFGVKAINF
                     LGEPGWARFSLIMMLVWKGLGTSILLYLAGLQGISDTYKEAAKIDGANDWQIFWHVTF
                     PLLGPTHFFIVVTGIIGGMQLYVEPELLLGGGPSRATTTLLIYLFERFGAHRISEASV
                     VAWVLGVFVFIITAIQFYTNYRREKA"
     gene            complement(8460..9905)
                     /locus_tag="PK460_00040"
     CDS             complement(8460..9905)
                     /locus_tag="PK460_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE53526.1"
                     /translation="MFKKKWMPLLLSIPLLLTGCKGKTSDSVTIFIYGQAHERSIYSK
                     IIEKFTEETGIKVNAELVNTDGYAETLTASLGTKNAPDVFYSNPASIGRYAMNGVILD
                     LKPYLEIEAEVEDGLDYKNFLDGVVDYYRYDVTTKKRGEGEAVWAIPKDVSSYPLGYN
                     RNIINLSVELGHWATHTKKGAWASREGGMPLPWENDPRTDKQIVYTFEEFTNACRATK
                     FIHNEIEYFGTALIDTWAMHSWIWAAGGNWLSDDGKTVTVNTPEFIAGFTEFIDLMDN
                     RGISQTREQMGVKPHYDRWMAGEVAFFNVGVWDVAAFESVPKELLDYGLMPTPRKDNA
                     SPEWYTYIGTLGYAVSSRTRVAQEAVKLALYMSMSTESYNRMTKYQTIQLPNNKLMVD
                     EYLSDETIKPENKGIFLDVIQKGHGKLHPTAMAYNSLWYDAFIAELSRVWKVTDDDPS
                     GEMSPAQYAAYVQPIMQAQLNIALADEAAGV"
     gene            complement(9919..11232)
                     /locus_tag="PK460_00045"
     CDS             complement(9919..11232)
                     /locus_tag="PK460_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53527.1"
                     /translation="MKKLRYLLILLSASLVLSGCKGGKVSEEETLPELTNMTVDGVIN
                     EEAYNVIPGRTAGVKAGVLVQYAVGREGVYFGLTVTDTKHQVSAPSGIVASDYVGLAV
                     DALAVPKSDDGVSESTKLFRVDTMGRYTYTTGNEYGAWVDVDSGVYEGVISGEEIPTI
                     AVNIVKDQYYVVEIFFTWEHLGTTEEAAKDANCLMYYIEHRDMGVDIHADANILAPAM
                     YNRLVYLGNRKGSNLPADPPEITIDGVLDETLWDTAFVTNSGNFKERVPDETAGDYRT
                     LAIWGEKGIYLGVEVEDPHIEAPNGTGAAYKNAGMEMRIHVYNKDGLPLNSLKWLFDV
                     WGPQWHETGAGGLASSFAPYAEYKFVISGTIDNNEDTDEGWSFEIYIPFSELGVTNVD
                     SDYIKILHAVGSHEQNNMLPQEYLDLHPDVNWDYPEDYPRISKPV"
     gene            complement(11301..12467)
                     /locus_tag="PK460_00050"
     CDS             complement(11301..12467)
                     /locus_tag="PK460_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53528.1"
                     /translation="MKKRRLLIPLIGLALLLTGCKTTSSEEKPVSSEQPPVTSVTTEQ
                     PPVSSEEPPASSEETPVANIKFGADPFNGTSATGVAGVGVALYYDATEDDVAVAFDKV
                     AFTVNKPSGVLFDPVIGLVEKLSVYYLRPMEPGTYTVTLTVEDSAGLKATATKEIVVS
                     TGDLSGDVDAIKAAIAAIYPDNIYTNLDYRTSEGVFNEWVVVGKNFTVFDREDYTGNY
                     ATAFIPFARFEGGEALQDFTISFKYTTLNEVWKLLFSFWTGEVGADNFAGDYLRLLTN
                     RNAIGIQGDAQQASEFQDEGEATGIPLKEGPVWIKFTRQVTKVGEGDYTVVFKLFTST
                     DGETFVNNTTATLVNQVNTAGGTAGLLTGFLPFSIDNDFIVEDLAVSGTVFTLE"
     gene            complement(12507..13964)
                     /locus_tag="PK460_00055"
     CDS             complement(12507..13964)
                     /locus_tag="PK460_00055"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOE53529.1"
                     /translation="MFCYHKTSASNNEKFFRKGIFLSITLLIATTVLTSCKGGTSVNS
                     EIRSSEFNNNEVVYPSNPAFGNNVTAHDPSIFKDDDGTYYTFGSHFAVTKSINLMQWT
                     QVANDGGANVLYARNWRTVLADAFAHVGERPGSTWAPCVHKFGEKYYMYYSLSTFGSS
                     VSYIGRVEGDSVLGPYDNSVEIVKSDGRGGPNAIDPELFFSKDGRLWLVYGSFFAGIY
                     IKELYASGENIGLPKEDGYGKLLWQGGDQGPEGPFIFYNPNTDYFYLMASHGSLSTNY
                     NMRVARSKNPDGPYTDPRGMSAENYRNSGLKLAGNYQFQGYPIGYAALGHNSILREDG
                     KYYVVYHSRYRSGASGVTGYHNQFVNQMFFNKNGWPVLAPNRYAGEELQVITMAQASK
                     DYDVLIHTGGDSEGFATSVTYKFSQNGDLLDAEEDAIGTWSINEDYYVTIKIGLNTYE
                     GVIIPQWNNDLQRAGLSITAYSRNGLSLWANEHFR"
     gene            14249..15256
                     /locus_tag="PK460_00060"
     CDS             14249..15256
                     /locus_tag="PK460_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE53530.1"
                     /translation="MPTIRDIARKANVSPATVSRVLNGDPTINVQAKTKRRIFEVAED
                     LAYTKHLQPKKNQQKNKFRIAIYHWYTIEQEINDPYFISIRIGVQRACRELGIAFDVI
                     YREDRITDPFEDQKYDGVIMIGSFTRETQTFISDRFSAVVYANSEDEDGKCDCVRVDF
                     RALTHDVLHLLINKGHKKIAYIGGRERVSSEPNKTPDPRELEYIEVMHQNKLYYKPYV
                     KVGEYSAHSGYELANELIDNNEGNTPTAIFCGNDSIALGVSKAIQERGFKIPDDIAIF
                     GVNDIPTLQFTSPSLSSVKIHTEFMGELALKLLNERMKKERSLKLSVNVPYELILRES
                     A"
     gene            complement(15258..16676)
                     /locus_tag="PK460_00065"
     CDS             complement(15258..16676)
                     /locus_tag="PK460_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOE53531.1"
                     /translation="MKETNKKTRLKGFMKTTVLSFQHMFAMLGATVLVPMLAGLSISI
                     TLITAGIGTIIFYFVTKRKVPVFLGSSFAYLPALMSIMAESGEVGSLAWQQNMGKLVI
                     AIFFTGVMYLIFAGVIRLIGVEKVRKMFPPRVVGPVIIVLGMLLVPSIFWNNIIAPYA
                     LYGAEAWKVWTTALITLLSIIAISAFAKPKSFFKAAPILLGFLIGYVYGAIVGLVDFS
                     TLKGSDIVIFQSIGSLLGFYKYLKFDWNAILMMVPIAFVTLMEHIGDISANSTVCGKD
                     FFVDPGIDCTLAGDGFAIMAASALGGPPQTTYGENTAILVITENYEPRNVFYAAILAV
                     IFGVISIFGNVIATIPSAVIGGASIILFGMISASGLRMVIEHKADIGQTKDLIVVSVT
                     LAVGLGLGALSVASDILSAVGVTTFDPRLLKVMIGNVELSPLAIATLVAVLLNLVIPN
                     PKVVKSEEKKASTEDTLTNEIN"
     gene            complement(16718..17218)
                     /locus_tag="PK460_00070"
     CDS             complement(16718..17218)
                     /locus_tag="PK460_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOE53532.1"
                     /translation="MEKWDLYTKDEKKTGKIVDKGALIPEGYYHIIVETLIKHTDGTY
                     LVMQRSYDKKEYPGKYEGSCGGSIKAGETKEEGAIREVFEESGLVINEVTPTYYFIQK
                     DVPAINQGYLAIISAKDKTIKYQKEETINHKWLTLKELKAFIKSDEYNESHASRLVTY
                     LDSLNE"
     gene            complement(17385..18458)
                     /locus_tag="PK460_00075"
     CDS             complement(17385..18458)
                     /locus_tag="PK460_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53533.1"
                     /translation="MKRKRMLWLPLLALLLGGCSVDFDFGPTSKDSEGGGGGTTSQTG
                     GAVSRDEANSRVTAAFTNSDIADATTYQQTTLQEVTDDTKEYVNDALKYHVVENTNSN
                     VVVKATGLQTDDPVAKVTGEPLEDMTEDGVETYKLIDNREFYYASEWIYYHNATRKRY
                     GNPEEPFIDTEVKLKQHMGPTNGQQLAEGLEGFDLGDAPVPTMFIGSELDVLQQTANI
                     TATKNGRNITVIYSINKDAFIAMMESGLRGILFSEEMTSEERAEAEEFLQWYVALLEE
                     VLDLREYKITIGADGNDHLTQVRADFELITTMVYEPSETYPKGYTQVGKLSGFLDMKP
                     KFNEEFTITLPDFSDYEEGIIFE"
     gene            complement(18730..19722)
                     /locus_tag="PK460_00080"
     CDS             complement(18730..19722)
                     /locus_tag="PK460_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53534.1"
                     /translation="MKLRNLLWLPIAAMLLNSCALGKKVEPSVAQERALAATKANEEA
                     EIDTQAITSKSEISDTIKSYEGETVVTETITSKFESSFKAKGLHGDTPEFAATYKEMM
                     EEKVDGDETYYVNHEEAFYLKDSWLFGGFSTTVRERGETETEENKGKVKIVPIDFKVV
                     SVFDYLSGFSAGEYQFGAPNEEGLAILFQVASEIEAKELFGKLTITYKLDVKSLVLIM
                     LLAFGLDLDAMTEEEREAFDTELNTMVEEMSKVMSINKYQMTLGISKDGYVEKIRTDI
                     DMVTNKYNEEVEPKVLVASEELKTFTESTMEINKKLTIKYPNIDDYPEAQPIIR"
     gene            complement(19862..20863)
                     /locus_tag="PK460_00085"
     CDS             complement(19862..20863)
                     /locus_tag="PK460_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53535.1"
                     /translation="MKFKKLLLLPLAALLLNGCAIGKKVDKDVANERVSEAIQTQEEM
                     DVDSLFQEAKQDMTESVSQTNLGAETTSKVTNKGTTTLKAKDLLGENPQVVASAKATA
                     KIDKNGQEDSFVSVNENMYYVDGWFYNDYDNKERGNISLAPKSGKGKIKINVGNIDFA
                     NKNVLAVFSELSGTAVNFGPVDYSQILLLASITGNVKAREFAGVLTITYNLSKDDLVR
                     FVAAQMAGIDYGDLTSEERELVDAIISEIIAQLEKGYEVRKYVIEVKIAKEGYITKVR
                     VENDVTQTITDPQGMGEDPEYVIVVKSKGFTEINTKLNEKVAIKFPDFSDYVERSLM"
     gene            complement(21133..21840)
                     /locus_tag="PK460_00090"
     CDS             complement(21133..21840)
                     /locus_tag="PK460_00090"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE53536.1"
                     /translation="MLLHQNIKEFRKIVILTATHYGLQEYQIEKDYYISLFLKPLGNM
                     SKNIPIVFKGGTSLSKCYGVISRFSEDIDLSVIQNKQLTRKQKKDLKQLIIETAKEIG
                     AEVININEIESRKHFNRYILKFNSVFNSDVDTLQKLIIETMIAYNPFPAIYQKTENLI
                     LEYLKIQKKNDIIGQYQLDSFLFLTQSIERTFVDKIFALCDYHLLNKYERNSRHLYDL
                     HMIWESGLLKKNTSITY"
     gene            complement(21818..22480)
                     /locus_tag="PK460_00095"
     CDS             complement(21818..22480)
                     /locus_tag="PK460_00095"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOE53537.1"
                     /translation="MKQKKINNLIIDYIFNNYDKETPIFTKDIYQKYSEISRSTIRSI
                     LSRLVKENNIVRIAEGIYSLPNYDCAIGTIAISNNEIINKKYIRNDGKIFGYRTGISF
                     ANKLGLTTQTAGVDSIISNCVANKKREVKLNKTRVIVNSPRVEVTANNYKLLQILDLL
                     NDYTSFSEINLEEGASTIFKYLEGVNLERKEVEKIVNSYPREAQVNFYKIRCQDAITS
                     KY"
     gene            22621..23169
                     /locus_tag="PK460_00100"
     CDS             22621..23169
                     /locus_tag="PK460_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE53538.1"
                     /translation="MKLATKRLILRPFTLNDVDAVQKYSSKESVTRYMMWGPNKYEDT
                     KRFVSSVVSRNSETPRDVFDFIVTLKDGTVIGACGLYFDEYPKPPTLGWVFDDTYWNQ
                     GYGTETGRALLAFGFNTLKVKSILATCDNENIASARIMEKIGMRYVETRPEELPKLGK
                     RFIKVYEITALEYKLNSNKVLL"
     gene            23166..24152
                     /locus_tag="PK460_00105"
     CDS             23166..24152
                     /locus_tag="PK460_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOE53539.1"
                     /translation="MKLRISSVLILLIALLLPFSRQKPLVLDHNDDPFVFHLQKESLK
                     IAQFTDLHLAFGFDANDRKTLKLISNITAETKPDLIVLSGDQTLSITAPARHRQLIRH
                     MESLKTPWTFVFGNHEADYHKMERLLKVIFTMNTKYMYFKVGPLLENGGYGNFTINYL
                     YEDNPFYNLYLLDSKAELKIPGSGRLFVYDYFSEAQVDWYRNKVMIDKINNIKSTVFA
                     HIPLIQYADALLIENKDKVKGIIGERISHQEVDTGFFNAMVESTVSEAFFAGHDHLNN
                     FTFEKDGIILGYGQISGYNGYGTLRRGARIIEIDESKMFKTYVVYEDLSYEY"
     gene            24142..24291
                     /locus_tag="PK460_00110"
     CDS             24142..24291
                     /locus_tag="PK460_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53540.1"
                     /translation="MNIKRFLMLLINAFVLFVILFMLTRTLWTLLVFVMPVVSLFLPK
                     EKSNG"
     gene            complement(24566..>25649)
                     /locus_tag="PK460_00115"
     CDS             complement(24566..>25649)
                     /locus_tag="PK460_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53541.1"
                     /translation="SETDVPSTESSSEIEGAQTWSVDDDFTWQTYEYALDSYNVPLYV
                     PTENPYIDIISDADKETFYTNNYKRASSYEDAMFRTEKGLISGDIIDTPNTANYPLNH
                     LPNRVYRTLANYRINEGVYEYTLDGEYKSYTINNINGKVKKVYYGAAYVSLDDVAAYL
                     FAFGEAPANQMMAKNSTAMNQAISLFGKYGRVNNGYYSSDVTKYKYEPSLPHTDNGGP
                     VEADMYNYREMDFGYTYTPWGYGIESDDPYNNGTKITRSTVRFVYTAYDNEGNEGAKY
                     IPVEHRHVFLTINHYNDFFEYLNYEGGWSESFGWMSAGNEYCAGMNSSKYGKGYYDFE
                     NPVPPTKYNIPQVKTLTELQSIFNCL"
     assembly_gap    25650..25749
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25979..27598
                     /locus_tag="PK460_00120"
     CDS             25979..27598
                     /locus_tag="PK460_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOE53542.1"
                     /translation="MNWLKRNIRYFILSLFLLTNGLIIFEATLAGGTSGARSSLLSLI
                     LSVFVNKTVDGPETAYVPIESLTLKTKDDTTLTEGMHFYIPLGITRRITATVLPENAT
                     DKGVNWTSSDSSALEVYPGGYLEARALSENVLVTAMTNSPLHRISFYVTIHEKVAPPE
                     YEVALTKDRITIDTTTQLEVSVDESVANEYDVRKLTFFSEDESVATINKYGVIKGISV
                     GETYVGVVGDDRKFKVSVKSNDTPLISPTSITLEFDEIAYVYDKIPLNYTFDVENVTD
                     PSLTFVSSNDIVATVIKEDDNYFIKTPKIKGTATITAYLNSNFAIRATKDIIVNNVMP
                     TSVNFTNVPTELITGQRVNVGYDLNHSLAKESLSVTDKRVIFTSSDNSIARVSTGNMF
                     GTIVALKEGVVTISATSVADPTISASFSIKIIPRPFINDDNFSDFQGFVRKAVGHFFL
                     FFIDGIFGFFTFYLFLKDKKSTRLITVLSLITGVFIAALSEFIQLFVPGRAGTITDVI
                     IDSAGYLCATMLMLLIMYLINKNKSNKNVQQ"
     gene            complement(27685..28158)
                     /locus_tag="PK460_00125"
     CDS             complement(27685..28158)
                     /locus_tag="PK460_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOE53543.1"
                     /translation="MIGINHHEIENEVTATLHAISVDNDFEFQLPLDVNAKTLGVTLG
                     VPVVISLYNEAFYYQITISDYRRSNFMQVSMRLFIGEIPNPQKLYALINKLNTKLLYA
                     KVVLDEEEDNTLILEHDFTAASVEQLSESIYDFLVDVVDEDVSPLIEELLKEIRD"
     gene            complement(28176..28913)
                     /locus_tag="PK460_00130"
     CDS             complement(28176..28913)
                     /locus_tag="PK460_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53544.1"
                     /translation="MILLLGTTPDDILYIKNRMIMKEKGAVNKDEHFYFVGEYGGKEI
                     CMTYTGHSNIMSAVLTSYMIRKFNPYLVISIGSAQSASSNLNQGDLFIAERVYIADLD
                     YNNYTNLPLRDAIHMHPYYVSEEVYIKHFELLNSATGNYKLVRGPVISVNNFVKEKAE
                     AERIIARQENYIEGPTVFDTEMGGIVACCRFYGIPWLYLRSINYEIGKEEQLISFVRK
                     GVEAQPRIGELVEQLFIFLNTSLEESI"
     gene            complement(28913..30499)
                     /locus_tag="PK460_00135"
     CDS             complement(28913..30499)
                     /locus_tag="PK460_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE53545.1"
                     /translation="MKQKKARRHSGKKYLWYSILCVMLLVLAFSTYVLMSFGLIPMTG
                     NKTLDIIIGILLVIISIASIVCGIIAFVYYKRNSNQIRFNYATFELVEGINSERAFLA
                     YVAKDIKRNRGKANALVAFAATRFKKIVFTRYGYEKGGEVLGLVYETFNTLKTKYPHS
                     YYGYDYNENFLIYLRDTPYEQVQEYVNDLIEEINKTLFENRNEVKFVADFGVSYCQNE
                     NDEFITAASMLQRALIAADHGRAESDLGGLTLYEPKMYERNKRNVQLGLQIERSLELE
                     QFELHYQPKYDLKLGRFAGAEALVRWRHPELGLLSPANFILFAEQSDLIIKIDHYVLE
                     KVCKQLDEWRGKGQRLLPISVNISKRTLFTGNIINHLIKVTDKYNINPMLLEIEIVES
                     PSLQDILLLLSIVKKFKNLKVKIAIDDFGTGYSSLSYIKKIPFDVIKIDKAFLDDVDI
                     DQKSRLMIKNIIDLAHILDAYVVMEGVQDERQVKMLEEMGADSIQGFYYSRPLPPEEY
                     QAFLEDNLFEANKKKKRGRK"
     gene            complement(30480..31289)
                     /locus_tag="PK460_00140"
     CDS             complement(30480..31289)
                     /locus_tag="PK460_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53546.1"
                     /translation="MPKHKQNSEAAPKKKGRIGKITSVVLGAIVVLLIVLQVVGQSTA
                     KNNHGVMRYGNYQSLRVLTDSMEPTYKVDTMVFVKRVDPKTLKGPSSEGALDGDVITF
                     VRNYHGSYDLPGDKRIVTHRIHRIEVQADGRVYFRTLGDNIHAKECPESGCTLEARGD
                     WVRDTDILGKVVGQSMVLGKFSVLLANPIFMLVFAMVPLLIVFGSSLMDLLKQLKSTE
                     GSEATPSGISEQQAEFERIKEQEKLRLLIELEKEKLRKELEEGIKDETKES"
     gene            complement(31267..33165)
                     /locus_tag="PK460_00145"
     CDS             complement(31267..33165)
                     /locus_tag="PK460_00145"
                     /inference="COORDINATES: protein motif:HMM:NF021352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HOE53547.1"
                     /translation="MADENKVTIKQEQNVVAEEVAKEETAPKTKKDKPLKVAGAQEKV
                     KKAPIKDKAKKAADTKPKQTKKVASKKSDTKVEKEKVDAKPKAEKAKKAKADPKKADA
                     KPETEKVAEKALDTEAPAAKEKEPKEAPKKEAPKKAVPRRRRLSAMELRKREALKGYK
                     RFTKVRSRYYSFLNRTVKQSKPTTNLYNTIANKAPTLSGITRNEVKRFDPEFIEKIER
                     VIPSLEEIVVNPHKFINEFAEVVQVEKARKLTPRAVKYMAQNADHVSEVLDDGRVIPK
                     KILNVYVDDDLKIYENRFIMTLINRLQIFIELRHKYIREHGDTRNSDQITIKREVQVE
                     DSLFTVDATITMSVPSDDEGARESNMDLLERLENIRRRTQFLVNSRFMREMAKAVPVS
                     EPIAQTNIIRLNWAYQDAFKLWNFIHRYEELGISYTTTQAKIEFDKKYLDKINQFSLN
                     SFFTIETEHARLALSNVEQKNIRPIIEAGDLNYELSDERFAKPGLPMRVDARVETAEQ
                     EAARLKREATREKARLKREEAKRKQKEREALKKARAKERALKRKLEAAERAKRRKEEA
                     KQRQIEKNMERARLRAEKERLLAERKAYQKLMMTEAERLRRARKAVAAQATEERKKAI
                     EDAKAQAK"
     gene            complement(33173..34693)
                     /locus_tag="PK460_00150"
     CDS             complement(33173..34693)
                     /locus_tag="PK460_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53548.1"
                     /translation="MNAFFSYIRDTFESFWANFVEWLAAVFVEPWKLVSDDFGTYRTI
                     IEMYMDSFGFWGWFFFVILMILNVAVIGGLIFLLVLLIRRLVKYSSKNYDKAKLIAEV
                     QRLNKELFFAIQEKNEVLKLKAESLGLEDPSLVEGDKTSVVIDTFPKLGAVDLKYKDK
                     DTDFITPVLDREITLEQIAVRYRYFAASQLGLFYDIDLVRVLFAAMGTVKIIILEGIS
                     GTGKTSLPYSLGKFFLNDVAICSVQPSWRDRTELIGYYNEFTKKFNESEFLRAIYEAS
                     YRKDVNIVILDEMNLARIEYYFAEFLSIMEMPNPQEWYVEVTKTADEMNPVQLVNGKL
                     LIGQNIWFFGTANNDDSTFTITDKVYDRAISITLNERGQMFDAEFTEPLKIPYEHLRR
                     LFDEAQTNFPLSKANANKFSKLDEYVVNKFRIGFGNRILRQIHTFVPIYVASGGTEPE
                     ALDFMFSSKILKKFRSLNLAFMRDELSALRAFIERTFGKNTFKHSLNQIDDFMTRA"
     gene            complement(34714..35937)
                     /locus_tag="PK460_00155"
     CDS             complement(34714..35937)
                     /locus_tag="PK460_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53549.1"
                     /translation="MAEEINLSEHPLLEVANDIIREEKRRKRTKKVVAVSAFIIIVFI
                     IISYLGYAIGYYIANATSLSLLSKDPEARFAQVGFRLSEPLTVTEQHKYNLQSDEGWV
                     GGEGYVYWTTQNVSPQTMSFVLEHEGYSNTNRLSPVTSGSFTKDEDPYLPLTLYEAPK
                     QNRAERHNLNLNVADPKDYFGFTVLFRLLIDEDRAGTIPLDNIAIYFNRDTKVHGQNE
                     LPKALRFGFQSGAKKDIISPGRTLLEENDYTGSIAVGGRLDLDDPRAPASLTGFYDYT
                     FGLDGAKYGESGEQIYEIAYGEFEQPLTNENWGEPLAEAEEKLEHGDVFHAVTYKGVR
                     PLINVVPQRQYYTSFQKYMLDGDKGEPLCYTNNLGVAEMNIKFWLEGWDLATTNKVVN
                     KSFGMQLHFVAKRAE"
     regulatory      complement(36125..36204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(36303..36695)
                     /locus_tag="PK460_00160"
     CDS             complement(36303..36695)
                     /locus_tag="PK460_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53550.1"
                     /translation="MTGQEKKKKFKLPSWATYLLLGLELALMVALVTISIVALKNVQA
                     GSGGPFIKWLIGNRVWFFVLIVLPLIVLFLFNIYLLIKVMNEASSKEFKQMTKDELLE
                     EARKQALEELRKELAKQNEPAPETKPEE"
     gene            complement(36887..37753)
                     /locus_tag="PK460_00165"
     CDS             complement(36887..37753)
                     /locus_tag="PK460_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53551.1"
                     /translation="MNKRNKRLGTVLTLLFISAIAATSGTLAWFTTVRTAGITYGDAE
                     VYMRDSDLVVTYKSSLNSLSATSELDGITKITLTGTNKVTDISGNGINFYKPRWNSDN
                     VTAAVINTVPSTNPGDADGYFIDFTLTLSRSPGEDPLNLYVFLGQNTAILPADPANPK
                     DVGALKALRMAVISYDNNDSATGTPSVDILYAPEAEINPEYLQLGSGGAYGLTTHELV
                     PATVKSDSFETHYTIADSQAVYPEIAVLPAGTGTADVTFRVWIEGTDIHAAESIIGGK
                     FKIKLDIYSTGV"
     gene            complement(37845..38699)
                     /locus_tag="PK460_00170"
     CDS             complement(37845..38699)
                     /locus_tag="PK460_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53552.1"
                     /translation="MNKKNKVLLGAIGLVLLSGIAATSSTFAWFTTVRTASISYSEAT
                     VQASDGNLAIEYLSSLNNWSGGSTYDAEANKVTIAGANKVTDISGDGKTFYKPVWTTP
                     VSTASSIETVPTTGSPGVADGFYIDFTVRLSRDNDVDENGLKVYLGTGTTIAPKDPEN
                     LADTGAVKAARLAVIDHEGDVKVRWAPEQETEPKYITTGTDSLYGVSGFETTTDLNYK
                     HGEITSADSKAAADLAGFLVADLSKGVATEANVTFRIWLEGEDKDCNNDILGGVFNVN
                     IALYTFIN"
     gene            complement(38825..39664)
                     /locus_tag="PK460_00175"
     CDS             complement(38825..39664)
                     /locus_tag="PK460_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53553.1"
                     /translation="MKNKKSIMFGALALVLLSGLSAASTTFAWFTTTRTASLTFSDAT
                     VVCSDSNLVVTYVDSLNTFGTISDEANEIKLTGANRVTDISGNGLDFYKPVWSPIEGV
                     ATNIKEVATAENYYIDFVLKIARQSDSPSATGIKVYLGENTKILPVDATKPRDVDAVS
                     AVRMAVISYADEFRASPALIELHAPVVETSPEYIIKDTASTAYSVNGYKVHDASAEVN
                     SDPFVTKLSEADGPTHQIADLSSDSEAYVGFRFWIEGTDAEATKDKALGGMFKVALDI
                     YSL"
     regulatory      complement(39793..39870)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            40188..41330
                     /locus_tag="PK460_00180"
     CDS             40188..41330
                     /locus_tag="PK460_00180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE53554.1"
                     /translation="MENFFVIVSAFFYLSIMTASFIFLNRQDDQNSRRFRVVVLLYVF
                     TILGIILDTLYRLDYGGLINLPSYAFAGVITATYVCLPVMWGLWCLYISHTSYLKEIP
                     RAYNIVVAATLLLNLALSLISLIPGTNIYFTIVDGVYARGKLFFVQTIIFLLFLALTI
                     CFYTRFFKTFGKSERFTFLLIITTLILALVGHLLNNFIPFMVMNNIFNYMILAMNVQH
                     IHASTDFLTGLANRRSLSNHLHSRMTGTGKTSSFSIIMFDLDNFKMINDTLGHDAGDD
                     ALRQFARFLRHHCDNVDFVARFGGDEFIIVLDSMSPAELKKRVAVIREQFSEYEGNKV
                     GNFKLVFSAGYFIHNKDLKISTGELIRIIDERMFRDKRKRSNGKTN"
     gene            complement(41393..42034)
                     /locus_tag="PK460_00185"
     CDS             complement(41393..42034)
                     /locus_tag="PK460_00185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOE53555.1"
                     /translation="MSTAQINPTRMELQRLRDKLATTTRGHKLLKDKQDELIHEFIPL
                     LPEYRALREKVESALSATLIRYQFACVKMSKEAINDHINDSVTKHELSAKFDRVMGVV
                     IPNLETKASHTEVNYDLLSTTSAFDDLITAIQDLFALLIELANLENTIKILVAEIEKS
                     KRRVNAIENIVIKEIEEQIRVIRMKLTDLERSNTIRMMKSKEIIMEKKFNEGK"
     gene            complement(42038..43420)
                     /locus_tag="PK460_00190"
     CDS             complement(42038..43420)
                     /locus_tag="PK460_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOE53556.1"
                     /translation="MAIKAFKTIKEVVGPLMVVENVSGVKFDELVRIIQSNGEERLGK
                     VLEIDNDKAVVQLFESSQGLKIDDAKAIFTGEGVTFGVSPHILGRKFDGLGRPIDGKG
                     HIIPFKRIDINGVPLNPVARDYPNEFIQTGISAIDGLNTLVRGQKLPIFSSSGLTHNR
                     LAAQIAKQATVLGRDEKFAVVFAAIGITFEEANFFSEAFEESDALDRTVMFFNLANDP
                     AIERIATPRMAITAAEYLAYELGMHVLVILTDITNYAEALREISAARKEVPGRRGYPG
                     YMYTDLASLYERAGRVKGLKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRPL
                     SMKGLTPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFSAYARGKDAKELSLILGD
                     SALSEIDQKYAAFADAFEREYVSQGFDTNRSIKETLDLGWKLLGMLPRQELRRVDPKL
                     VDKYYKESKE"
     gene            complement(43425..45185)
                     /locus_tag="PK460_00195"
     CDS             complement(43425..45185)
                     /locus_tag="PK460_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOE53557.1"
                     /translation="MAKVTGKIVKVSGPLVVASGMKTAQVYDVVLVGDKQIIGEIIEL
                     RDDLASIQVYEETAGVGPGEPVTLTEEPLSVELGPGLMEAIYDGIQRPLTSIYELHGT
                     YIPVGVQVPKLDRKRKWNFIKTVKVGEEVEEGDELGYVNETPAVKHLILVPPGVKGKV
                     TKINEGEFTLEEVIAELETIDGKKELTMLQKWPVRKKRPYLTKKPPKEAMVTGQRVID
                     TLFPIASGGIAAIPGPFGSGKTVVQHQLAKWADADLIVYIGCGERGNEMTDVLMEFPK
                     LVDPKTGLSLMNRTVLIANTSNMPVAAREASMYTGITIAEYFRDMGYRVAIMADSTSR
                     WAEALREMSSRLEEMPGEEGYPAYLASRLSDFYERAGVVVTLGKKKRLGSITAIGAVS
                     PPGGDTSEPVSQATLRIVKVFWGLSASLAYKRHFPAIDWLRSYSLYDDDIVKHHSASF
                     PYWKDYVALLKELLQDEAALLEIVRLVGIDSLSQKDRVKLHAAQMIREDYLHQNAFHA
                     VDTYTSIEKQYGMLDLIVTWFKEANLALDEGVEYKQMENLAVNERISHIKYVEEKDFD
                     KQVKALKKDVIAAFNNLKEE"
     gene            complement(45198..45500)
                     /locus_tag="PK460_00200"
     CDS             complement(45198..45500)
                     /locus_tag="PK460_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53558.1"
                     /translation="MDELVLFSNKSEAILFKAIGFLVHVVKDEEEITEILKELPKEVK
                     VIAYDTFFTSFFTEYTKNQKQLYPLYLPLPFNTEDEGKAISEMKESIRKSIGIDLL"
     gene            complement(45504..46220)
                     /locus_tag="PK460_00205"
     CDS             complement(45504..46220)
                     /locus_tag="PK460_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOE53559.1"
                     /translation="MNNVYLKGVLDAKISNLLTESDFATFANLSGMDRLNFFINNELV
                     STSIVTNFEALCKLALSDLKEEVSLYTKNDPLYVNYFFAHDVIKKDQGALRDFYNETY
                     EKALQRKEEALVNYLDYTHALLNILTLVRGKKRGDTTASLLANYLKQGVLSEETFSAL
                     VNGDTHTIYAYLKNNFNIDASESESTVELEEKLDEYLFNKLKILAMDSDFKPTLIYYI
                     KRKQLQIERLRNIVYTKRNG"
     gene            complement(46213..46824)
                     /locus_tag="PK460_00210"
     CDS             complement(46213..46824)
                     /locus_tag="PK460_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53560.1"
                     /translation="MKKPIFERITEEAKKEAALLVKETKSEASRILKAGKDAISAQNE
                     SELTEAKAKSVERVRQYKERNEKGLDNYKEQVRQEIVVSIFDEVVNKLANLEGEELLK
                     FVTNLIAKEKVKGDEVIHVSARNFKKFVTALSSKKDATNLDLLNKKDAKYNFTLSKEP
                     THVAEGFLLSTPQYDLIFDFKEIVINYQKKHEQRIYNELFKDE"
     gene            complement(46834..47307)
                     /locus_tag="PK460_00215"
     CDS             complement(46834..47307)
                     /locus_tag="PK460_00215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOE53561.1"
                     /translation="MTTGLVYALIGAALAAGLAGVGSAVGVSLGGKAAAGVISEKPEL
                     FGRVLILQALPATQGIYGFLVAVLIMVKIGMIGGNPIALTNEQGLALFAAALPIAIGG
                     LASGIYQGKMAAASISMTGKNPAMSARGMTMTAMVETYAILALLVSVLMYIAVPV"
     gene            complement(47308..49215)
                     /locus_tag="PK460_00220"
     CDS             complement(47308..49215)
                     /locus_tag="PK460_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOE53562.1"
                     /translation="MIVPVKKIRLLTAISEQGKLIEALQHESKFMLFEHENDPSLPSD
                     INNELRAIERGIKALSKYKKKKFFAYHEVKQSEFNTIEAEGKALLSKINSALKTIENA
                     EKEIAELQAEIKTLTPYLNLSVATKPLSELVRTRIVIGQVPLNLFEDFAKEADEKGHI
                     FEESSRDHEFVYGAFGYEVVYESQALILLNSYRFTEVTLPLYDTKLKALLPELNDNLA
                     NKDKAILEAKDSLKALSNDEEKLRIYYDYYYNLALRKLVVTKTTERVAYVEGWVREDE
                     FETLTKNLENKVDCDIEELAVQNEEMVPTATKNNKLVKPFESITNMFSVPNPNELDPN
                     PAMSVWYWIIFGIMMGDMGYGLALLIIFGLFLKLAKPKGGLKDLATIFLYSSIPSIIF
                     GILTGSFFGVSVDLLNIVGKWFGNPNLTSVVLQPVEDPLPMLIFSLALGIAHIITGLV
                     LKIINDARKRDIKSMLADAVSWILILVGLVIAVVVKNQIVLYVAYGMIGIGAIFILLF
                     SGRDKKGIIGKALAGLGGLYNVTSYLSDVLSYSRLLALSLSTAVIAFTMNLLAGMVGG
                     IPVVGILFAAIVYLIGHVFNFAMGLLSAYVHDGRLQYLEFFGKFYEGGGYEFTPFSYK
                     LKYVNAIKENE"
     gene            complement(49212..49520)
                     /locus_tag="PK460_00225"
     CDS             complement(49212..49520)
                     /locus_tag="PK460_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53563.1"
                     /translation="MSVLEKIVQAELDAKELLAKTREDNTKLLSDTHDELATLRAENA
                     LAIAREINALNKKADHEIEKLATTFAAKKETITNEIEKSAKANQSKLVDKVFKDIVEK
                     "
     gene            complement(49719..52160)
                     /locus_tag="PK460_00230"
     CDS             complement(49719..52160)
                     /locus_tag="PK460_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE53564.1"
                     /translation="MEKNGQILVVSNNFNTKNAVKTALENDFILFFSTPSEMFNEVEK
                     HPELCGVIFELKDENIQSVEQFSRLYLKYPRLKLLVLASKEFYESIDEEARFGFWCME
                     TSTAADLLNKIYELKEYPLSTIEKISLVYAAFERAPFGIMIIPNDNLELALFNRKNDE
                     VLGYTGEEYKKLGLNKETHPDDLQRVAEIEKQAKLLGENSFNVTKRVIKKDGNIVWVE
                     QFISIIKSEKYEGFSRIVISWDVTKQKAVEDTLAESDRARATLLENLPGMAYRSLNDK
                     NWTMEFVSDGALALTGYRPEELIDNKTVSYYDLIVRVHRPRIRQAFDEATKSKKQFRQ
                     EYEIVTRNGTKKWVSETAKPVFDKKGNLLFYEGIITDINHMKKIEKQLKHFQQYDQKL
                     NLPNREYLIDKIQAKLDSKNYSGTIILVNLKESQRLYRSHGYQYMELLTASLVTKLKT
                     IETSRITLHYAEEDLFVFYAKYQMTREEIKKFYAELKSAIGHTISREQIRSGIGVTFL
                     HKSISIAEVYLQRARIASEFIDDENALISIHIYNDIMDQKVKREEHLRQELIAISYEP
                     GNEHKLRLMFQPIINLQTGKVRGFEALARFTSDKYGPIAPSEFIPIVENNRLIIAFGK
                     KIINLAVSFAKELLATGIHDCGIAINISVLQLLDPNFAQDLFARIESAGVPPEMIMIE
                     MTESIFSTNYEQLSEALAEIKDYGIDTAIDDFGTGFSSLARIERLNFNTVKMDRVFVT
                     PINAENAEQGIAQDIINICKKFNIQSLAEGIETKEQYDALKGLGCEFGQGYYMSRPLE
                     VDAAIEYIKNAKRKK"
     gene            complement(52416..52892)
                     /locus_tag="PK460_00235"
     CDS             complement(52416..52892)
                     /locus_tag="PK460_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009986389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOE53565.1"
                     /translation="MNFNDFSHKDLRYYLAYGSNLNLAQMQERCGTSKKVGKAIIKDY
                     RLMFKGSENSNYLTIEKAKGYQVPVGVFIIQEGDEVALDRYEGFPTLYYKKDFVVELI
                     SENGKKTTINAFVYIMHEDRILGAPSEEYIRRVREGYKNFNFDEKILDEAIVFSAK"
     gene            complement(52917..53453)
                     /locus_tag="PK460_00240"
     CDS             complement(52917..53453)
                     /locus_tag="PK460_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE53566.1"
                     /translation="MEKEVMVNGKTYTLIKLLGKGKGGYSYLATFNNKEVVVKKIHHE
                     PCEYYTFGNKIEAEYYDYQRLFKAGIRMPRLIDICKGQEIIIKEYVKGDTIFEYVKRD
                     VNIDLFIDQVREMAALAKKAGLNIDYYPTNFVVQDNLIYYVDYECNEFSEEWNFENWG
                     IKYWTRTKEFKAVLKKEQ"
     gene            53538..55016
                     /locus_tag="PK460_00245"
     CDS             53538..55016
                     /locus_tag="PK460_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOE53567.1"
                     /translation="MKIISKSNYTLFKNCPKSLWLSFYKSDVAAPLTMDVLKRIEDGK
                     EVGDLAKKYFANTIDVKSLLPDGSLDISKMLELTKKYLKQKGVTIAEASFKVNNLFCS
                     IDLLRVTSEGYEIYEVKSTTEAKVEHKVDAAFQKYVLQQYGLNVINTYILHLNKDYIR
                     KGDLELDQLFTMNHLDEDTSFIEAMHNMKSDLKAIDKLLLTKAEPAETLLTRCKNCDF
                     KDYCQKDIPVPSVLDVYRLSGYKYLNKGIVTFEDLLESNVALRKRQSIQVHAYLDNKE
                     VIIDKKGLASFLKKIKYPVYHLDFESYQMPIPPCNLMKPYDQIPTQYSLHIEYEDGSL
                     EHKAFLGNSLDPRREIAESLCANIPRNACVTAYYKGFECGRLNELATLFPDLEEHLLN
                     ISKNVIDLLEPFESGYYYHKNMGKSNSIKAVLPALYPDDSELDYSALPVVHNGSEAMV
                     MYPIMLGAKKEEKERIRNGLLEYCRLDTLAMVKLLQKLRKTQ"
     gene            complement(55020..55931)
                     /locus_tag="PK460_00250"
     CDS             complement(55020..55931)
                     /locus_tag="PK460_00250"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOE53568.1"
                     /translation="MKKVFVLGSINYDVVVYTDKMPILGESIIGQGMIANLGGKGANH
                     AIAAKKIGVDKVFLISAVGDDDSGHIMLKSIKEHGVDTSGIEVIKGEPSGTCFIIFNR
                     SAEDNAVIVGNGANLMNAPQKAIDLLKKYARKGDIFIAQLETNLEALYKGIDFAHELG
                     MYIIINPSPVCEFNKSILSKVDLLILNAAEAKLLTGVNYKNNNDLQEIHDKLKAKSTI
                     VTFGEDGAYLIRDNEIIYQLAIPTNVVDTTCAGDTFLGALAYRKANGYEIEDSLRFAA
                     TASSIAVSRKGATQSVPYLNEILELYK"
     gene            complement(55962..57008)
                     /locus_tag="PK460_00255"
     CDS             complement(55962..57008)
                     /locus_tag="PK460_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE53569.1"
                     /translation="MRKFDYLFLKDGSVPASLIGLISSIYSLRTLDETRKHKYASIYT
                     KLIKIAKVQSVKGSNAIEGIVTSDERIKAIVNQNSAPLNHDEEEIAGYRDALNIIHNT
                     YENLQFEEKTILDLHRLLLAVSSPENAGRYKTSDNVIIEIKPDGTRNVRFRPTPTSET
                     APAIEQLILAYTIAVAESGINKLLLIPCVILDFLCIHPFSDGNGRVSRLLTLLLLYKA
                     GFDAAKYISFEEQINKRKGFYYEALRKSSVGWHENNNNYFFFIENFLVTLFYCYQELD
                     KRFLTVNSEKVNKTARIEATVLNSIVPISKREICEILPDISVTTVEYVLGKMVKSGKV
                     RKIGKSSNTKYFRM"
     gene            complement(57967..58683)
                     /locus_tag="PK460_00260"
                     /pseudo
     CDS             complement(57967..58683)
                     /locus_tag="PK460_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(58809..59738)
                     /locus_tag="PK460_00265"
     CDS             complement(58809..59738)
                     /locus_tag="PK460_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOE53570.1"
                     /translation="MVKNKVAVIIDTDLGADCDDVGTLVLALQLEKHGFCDIKAVGYC
                     TTNIYGVQCVDIVRSFYGGKFPIGMYYGKKVECNEDQHIFDIDLVDHYKDLIKETNYI
                     EAYKLYRKVLAEAEDDSIIFITIGELTNAANLVSSLPDEISPLSGQELFAKKVKYSCV
                     MGGLINDNHPITAFNNNEAFPEYNIVCAIEDAQKFVKISAKPIYFLDFYVGVDVFTGK
                     KVIEHDEHSPMKFAYLKYANGPSRSWDLLAVYYAITKRLYRSVGPGVMHIDDNGFTTF
                     TKDQDGHHYYLRPIKSNEEITKEIDEALVGNIL"
     gene            complement(59725..60429)
                     /locus_tag="PK460_00270"
     CDS             complement(59725..60429)
                     /locus_tag="PK460_00270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOE53571.1"
                     /translation="MFPNNTHKAITFSFDDGVTQDRRLVALFNKYNLKCTFNLNSGGF
                     GKDGVCHWQKWSAPHNKVNSAEVKALYSGHEVAVHTVNHPDLTTIADEKIIIDEIEQD
                     RKVLEKLTGDKIVGMAYPFGTLNDRIVNIIKRNTPIKYARSIWATYNFDLQDNLLAFR
                     PTIHFLDFKKMYELAGTFLNTEYYEDKIFYIWGHSYELDFEDAWEEFEKFLAYIANKK
                     DVFYGTNKEVLLNGQK"
     gene            complement(60431..61318)
                     /locus_tag="PK460_00275"
     CDS             complement(60431..61318)
                     /locus_tag="PK460_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53572.1"
                     /translation="MSNKGKIKLKANTAYKISFDTKGYRVGGNGAYYFLKLRSGQENI
                     MTIGEWYDRPDAPANNKIFTFTTPENSKDLTIVFGIKNEGGYYVDNISLIELPNGHIV
                     DGEDVGFDNNVRPFTPNKGLGYEEGFEDGVLADSLFSFGFNRWGHFTNKPNEVINEKL
                     SFTSELDEVTYQFQDKNNYYEFMYSNDKYLKLSGNSTYRLTLKYKLMTEIRLHSDPSL
                     KGYAYIFARSKATHVDGNPDNPLLVGTEIHFASASTLETVYTETFELMTPSCDDTMII
                     IGLYGIGTMIVDDILVEKV"
     gene            complement(61549..62724)
                     /locus_tag="PK460_00280"
     CDS             complement(61549..62724)
                     /locus_tag="PK460_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53573.1"
                     /translation="MEMYGGNFIKLIIPIILASLSLGGFLTPNKSLVEAKQNDATRET
                     VVYHEDFENIVGEIYQDTKFWGNGMSIVENAGNHSLLYTYDGGATQIGGINTHQTGLT
                     GNDLFENGKLYRVEFDALVTSVADNATVQFDFNIEGLWTSFKVGKTTAYKTESSNGTV
                     RNVQYVDGQVSATFTCSAGGHYFEIFVANMASGDSFKIDNFKVVCLDDVYSTDNYGTI
                     AGSSQQDHYYVADGTLEPATAASGQKYLHFYGTNNTVDTWEAMFFNHLPLTYTAGKNY
                     RNVIKFLEAPTTTEMYINMVGNWAGHTTFTINPVTGEVTHDSGLETCDITWSDNTLVI
                     DFYCSTTLPEQQIRFVSKSIAGTVSDFKIAEFTVYDLDLLTAKAFAKDLLNKTANTT"
     gene            complement(62819..66046)
                     /locus_tag="PK460_00285"
     CDS             complement(62819..66046)
                     /locus_tag="PK460_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53574.1"
                     /translation="MKTHKFKNFFSKVWISITVFFAFVLTIGYPVYAYFRANIERQVE
                     PTLEIELLFGHLREDLVTGEWGTDTNPYLIMSTDHLSNLSVLQNGAYSHYVNENSVFQ
                     VSDENGDPIYVGGATANQLFTIQSIGNEDYPFVSKIRGITTTDPNKYVTLPTGELTDT
                     SIIGNVRVTPEENQVDIGLFGCVGPDLEGEVPETDYQGEISNILLYNIQINTSTVGVM
                     PSGHYGYAKTGVTKETNHLGILAGHVQYANLDKISVYYSQTNGVAHVDAFNVSAGDTA
                     KYTTSGGIIGFYKRVIVDGEIENPVTSDGSDQGAGQAAAGLGKGIVYAEDIWKFMEEN
                     TTVGNPAPNNSYDLQDTFGDELYDPNDNSYFHVGVFTFAHSRQSRGKDRIAKIWTDAY
                     TNEWTISTNPTNPYTQTTRDVGPATKYTATRITNNEMSWNYTGWFNRQYYGTLNSPYS
                     SNADYRYMLVYEDTTVNPTKHYALVRYGSTAIGVEVDPNNLVIPDGEFKYFTYENLSQ
                     RTADITYPPYEAGFSYRFSGPITMDFRNTTSSAGTLQYAVYGDIVTGIVDGQTKILEA
                     PRPLRVNAQSNAVPTSSFMATASTSNPPYVEGYRISPTSNTGNFNKFYIQRTGASSSG
                     GSTSNWYMTYSTAEGFSAVSTSSPGTYASSFALYAVRYTNNTNNPSESPVATRNILEE
                     FIPAGTTETIDTEHNVLFYTGTYNSSVPTSRYSYEIKDISSLGWIDNRNKAITKGDTA
                     LLIGDPTSYYYIKNASGLERFFGVNTGIPSPVDGPPINVPEGSIGFTVNAKTPDSTET
                     TATINVIVATDPSQGVEQLITVSRFGSGTTTVGDRQLLSGFVLPPPPGSTIIGTKPVY
                     LKTSASSPTYAVYPNFNRLLIAYSFEVPIETVPITYFLEASRGTACFVYLSSDRVAAN
                     DNNPLHENDIVIPSLPNIDYVFKNTTNMIATVGRTDYVPSMTMPYFGLTTNPNYDAET
                     NPNVDHYLVTAATGFDFVYNISRIYDPSQDEKYFLYIKVHANVNGSGYAAPVDLNSLK
                     IIQQNMNFNYSENSYLDRNTNEFIHSDVVHFTINGYFLDDWSVLE"
     gene            complement(66033..66731)
                     /locus_tag="PK460_00290"
     CDS             complement(66033..66731)
                     /locus_tag="PK460_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53575.1"
                     /translation="MKEKKRNIVRTIWLMVLSLVASASLTYAWHISVYNFAQTHNVPG
                     ELTPEISLAHYNYTTSSWQNSTSAEPLSIYLGKMTNIAQIPANSNTYLKIRINETSDA
                     QTKYNVIVNNIETVISNIDGVHAVTGVDYFAAENAQNVFDFYVYTSTSDNLNPLTVFA
                     NLQTMTPFKITTLPHALLSNSATLDTWIYILLKPDIAKVQNIIRLVPIQLSPYNMVFR
                     MSLNGEVKTLDENA"
     gene            complement(66733..67476)
                     /locus_tag="PK460_00295"
     CDS             complement(66733..67476)
                     /locus_tag="PK460_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53576.1"
                     /translation="MDSQNKIKKVAKLPSRKFRLFLTFLGTAVSTVALTITTLAWFMS
                     TLKGGAEIPDIGVDDEYAHSFSCALNSVPITNGSVEFTPFYPGHVHRKTLTFSLTNLS
                     SKTFKMNLFFEQPAALEEVPYIDATGKWGNANYYYYLGSQIALTSVSLAIDGASPTFT
                     SGAGNHLVETNSVGVVKGQVNGVANELTSIARFYIIQNVSVPANKTLTGTLEFTFVDN
                     GTDQSMYFVDWPTTGVSRRSLSAFFYRGI"
     regulatory      complement(67526..67603)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(68284..69387)
                     /locus_tag="PK460_00300"
     CDS             complement(68284..69387)
                     /locus_tag="PK460_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOE53577.1"
                     /translation="MKKNKKISKKTRENWRYLVTHFTPEYIEAMTDKKEYVVGWGSDN
                     KSFCYLVEVGTKELGEIRGANSSKFGLWYGTHGEDKEIKYRATKQHFNCSVNKAFEDI
                     KIALAKLIRETRELTEFKDLESLFSGMFKYKIMYLYNPSIMIPCFLLEDLQYFEEYLI
                     GKSSKTFENAQKALIKYKDKHFSKKDNHDFMIFLYSIREKYNVIQTKELNEEADNKLN
                     KKLSKEKESTYIYITHPVDKVSLKKGTGGGLYYPRDVKMAAIALKNANYKCEANSNHK
                     CFLRKSNGMPYTEVHHLIPLAYHYKFDKSIDIPENIVSLCSNCHNEIHYGRDANKMIK
                     KLFNKRKKKLHEAGVDVSLKELFEMYHKISGQK"
BASE COUNT        20984 a        13771 c        11217 g        23865 t
ORIGIN      
        1 ttttttacaa agggtgcctt tttataatta tttaaaagtt tagtgcttat ttcttttgac
       61 cataacgagc gttaggacca tgttttcgct cataatgggt tttcttaata tgctctggta
      121 gcggattatt ttgacccggg attgcttctg taagataggc aatcttcgct acttcttcta
      181 acacgatagc gtgcagcaca gcatctttag catttttacc ccacgcaaaa gcaccatgtc
      241 ctttgatgat tgccgctggt gtttcattag cccgtccttt tttaatgagc gccgcaatta
      301 cttcaccagt atttttttcg tagtctttca tctcttctgg tgttaaataa ggagcgcacg
      361 gaatatcatc gctaaaataa tcagcgtgtg tcgtaccata cattggtatt gctctacctt
      421 tttgtgccca cgctgtcgca aatgttgagt gagtatggac aattgcacca atttcttgat
      481 gtgctttata taaggctagg tgtgaaggca aatcaacaga cggtcgcaaa gttccttcaa
      541 tgatattccc gtcaaagtcg actacaacaa tatcatctgc tttcatcttt tcatacgcaa
      601 caccagacgg tttaattgct attactttaa gtacatcatc tttaacagac acattgcccc
      661 atgttaaaag tactaaaccc gatttcttaa tggcaatgtt agcgttatat gtcgtttctt
      721 ttaaagtttt gtacatattt taccctcgta aacgatagcg gatatcgtta tttttcatca
      781 tttcttcaag tatgtaagga tcagcgtctt tcccaatatg cacaaattca atgtcaagga
      841 gattagcaaa gtcacggaga aattcaagtg ataggtcata cactagtgtg gtgtgatgtg
      901 ccccgcccgc tactaaccaa caagctgctg acttttctaa atttggttct ggtcgccaca
      961 taaggcgcgc tacaggtaag tttggcattg atttagttgg ggcaacacat tcaacgtcaa
     1021 gagcaataag ccggaaacga tgacctaaat caattagtga gattgcttta gctttaccag
     1081 gcttaccttc aaagacaagt cgcgccggtg cttttttgcc accaatacct aagtgatgga
     1141 cttcaatttt cggtctttca cgagcaattg acggacatac ttcaagcatg tgtgcgccta
     1201 atattagccc ttctttccag tcataagtat aatcttccat aaaagtagta actttaccaa
     1261 ttgctttaaa gatacgtgtc atcgctgctg ttttccaatc cccttcagcg ccatacccat
     1321 aaccttctaa cattaagttt tgggttgcta gtcctgggag ttgttccatg ttatagagat
     1381 cttcgaatgt attagtgaat gctttagcgt tattagcgtc aagaatcttt ttaatcgcaa
     1441 cttctgcttt agcttgatat tcaattgatg caaagttatc agtgttcagt gcatactttg
     1501 ctttatattc cgctagtttt gctgtaagtt cttcgttagt aacggctttg attgtatcga
     1561 ctaatgtacc aacaccaatc gtatttactt gccaaccaaa tttcatttgc gcttcaactt
     1621 tatcaccttc tgtaaccgct acttcacgca tattatcacc aaagcgaacg acacttaatg
     1681 tcttactttc attaactgca tcaacaacat cttgccaagc agaaattgct tcgcttgttt
     1741 taggatcttg ccagtaaccg acaattattt tacgattctt ttgtaaacga gtaccgataa
     1801 aaccgtgttc tctatctccg tgcgctgatt gatgaagatt catgtaatcc atatcaatcg
     1861 cgtcattagg aatttcatca taaaactgtg tgtggaaatg acaccatggt ttttgtaaat
     1921 tttgtaaggc attcacccac atttttgatg gcgagaatgt gtgacaaaaa gtaatgatac
     1981 caaggcaatt ttcatcataa tttgcgtctt taattaagcg cattgcttct gcatgtgtct
     2041 taactgtctc cttatagacg ataggatatt tcgttttcag tccttttgca attttctttg
     2101 catttttttc tacttgcttg agggtttctt ctccatataa agattgtgac ccaacgataa
     2161 accaaaatgt tttcataaat tacgctcctt taaatacttt ttctatgaat ttatcaaatt
     2221 ttattttttg ctctttattg taataacgat atgctgctaa gacagccatc ccccacgggc
     2281 ccccttcacc agcggtctct aataaacgaa ttggaatacc aagtgtttca ctcatcactt
     2341 tcctacccgc gctctttgat ttaaagtatc cgccatgccc taataaataa tcaactttaa
     2401 catgctcttt gtttagtaag atatccatgc ccggttttaa gtcaattatt gcttcaacaa
     2461 gtttctgctt cataaattca ccaagcgggg tatcttctaa agaaacttca taaagtgttt
     2521 caaaaagatg attacgatca acttcaccat taactctcat aattgcttca ccgaaaagtt
     2581 caatccatgg atcaatacgt gaagtacatt cattcgcatg gaccattgcg actgcatcgc
     2641 ctgttggagt ttggacaata tcaatttcgg gataccattt attcaacgcc ttttctaaaa
     2701 caatcattga aaaaacagaa gtacccgcgg atacattacc cgttttaggt ttaagggcgt
     2761 ttgttgatac catccccgtt tccgcatccc cctctggtgg cgctaaaggg ataccgactg
     2821 ttaatttatt ccctaagagc tccgcacctt ttgctgttaa aaagccagcg tcttctccta
     2881 cttcaacaat accaggcaaa agcttttcaa gtttcatatc tgttaaatcg ttaaaagttt
     2941 gaatcatttt ttgattatag tgcttagttt taacatcaat ggggaacacc ccacttgcgt
     3001 cgccaatccc taatacattt ttaccggtaa gcgttagatg gacataacct gctaatgtag
     3061 taaaagtatc aatatctttt acataacttt ctttatctaa ataagcttga taaagatggg
     3121 caattgacca tcgtaacgga atattaaatg caaattgctt tgataactcc gtggccgcta
     3181 ctgttgtgtt cgtgtttttc catgtgcgga atggtgttaa taattttccg tttttatcaa
     3241 atggtaaaaa accatgcatc attgctgaca caccaagtga tttgatttct tttacattaa
     3301 tcgtcttagc aatttcgtca attactgcat tcatcccgac ccaaacttca ttaagatcat
     3361 aagtccagta cccatcttta agtgttgatt gccaaatgta agatcctgaa gcaatgactt
     3421 caaaattatc atttaagaca atccctttaa ttcttgttga gccaaattca attcctaaat
     3481 acacatttct tcctttctaa ataatacggt tgccccataa tgctgttcca tcactattaa
     3541 tcagtgataa gcccagcact cctttttcaa attcataatt aaattgcggg gtaataactc
     3601 ctttgtataa caccccattt aattcaagtt caagttgatt gttattaagg cgataacgac
     3661 cgttatattc tccgtttaca acataccatt cacctgcttt ttgcttagtt aatgtaatct
     3721 cttcagcgcg attaacaatc gaaataatgt catcagtaag cattgaatta accgcggatt
     3781 gatgcaggat ttgaatgcga taatttccgc ttatatcagc ttgttctaaa ttaatactac
     3841 cttcaccagt ataacgatta ggttctataa cgggccaatc attaacaaaa tgcatcttcc
     3901 aaataccaag aaagaaacga tgacgatcca tataagtgcc gcggtaatgc gatgcaaaga
     3961 accactgctc ttcatcttta aatgccgttg agtgaccaat acctgcccag ccacgtttat
     4021 aactaatatc atctgtaaga tatgtaaatt gatgggcaat tgtcactttg ttgccataag
     4081 gtctaaatga attataattt gttgcactaa gtgcactaac acccttacca tcaacataag
     4141 ggcctaagat attgcgtgag cgaaaaacac gcacatcata gtcataagtt aaatcaacac
     4201 cgttcgtaat aaataaataa taataaccat tttctttaat aatgtaaggc gcttcgctag
     4261 atgtaatact taatattttc gtaccaaaag caggatcatc gctggaggag ggcactaatt
     4321 tagtagcggt tgccgttaca cctttttcag cagtgttaac tgttacttcg cgttcgacaa
     4381 gtgtttgggc aataagcgga taacccgtca caggatcaag ttcgataatg taaatgcctg
     4441 ctgaccatga gccataactc atatacatct tttcttcgcc atcaacttct tccacaaata
     4501 tagcggaatc aatcgcattt ggcgtgccac ctacactttg gcttgtacgt aatatctctt
     4561 tatcaaaagt gtatggtcct tcaatattgt tagactttgc taatcccatc attgaattgc
     4621 gttgtccaaa accactgatt gaataaaata accaaaattc attacctctt ttaataatgt
     4681 ctggcgccca aaagaaatca accttttccc cgtctctgtt ataaccaata tattcaattt
     4741 gatcttcaag tgttgtaaca tctaacgccg ttcctacaaa actccattta attaaatcat
     4801 ttgatttgcg gatatgaatc cctttttgtg aggttacacc aaaattagcg tctgttgaaa
     4861 aagcgtaata tgtatcacct tctttaataa ttgctggatc atgcgtgtta gccgctcccc
     4921 agtagcgagt gtcgttaccc gcaatgttat agaaggcgta aggatctttt tgcataatgt
     4981 gatcactatc actatatggc ttagtaggat aaataattgc ttcttcaata ttgtcactat
     5041 tagaaataga ggtaattgtt ttacccccgc tacaagcggt taaagcaagg atcgccacac
     5101 taattaagaa aagataacgc ttcaaactag tacctcccta gtacttaaaa cgatagacat
     5161 gataggaaag cgggcctaat tccaaggtcg caacatcatt tttaactgtt ggtaactcac
     5221 cttcaacaaa tttgacattt tcttcatcaa aggtattttt aatttctaag tcgtcatgcg
     5281 tcattacttt atgagtaagt gcttctacac gaccaaacga acgtaattca agttcaactt
     5341 ccatcttctc ttgcgcataa tttacaagga atacagaaat ttctcgtttc tcttcgttaa
     5401 atgttactgc ttctgaaaca taatttactt caccgaactt tgaattaaat ttaggtcccg
     5461 ttttaattgc ttttaaagcg acaccacgac cataatttga cacagcataa aacggataga
     5521 aaattgtttg tttaaacacc cctccacctt cttttgtgaa aatgggggca ataacattta
     5581 caagttgcgc taaagcagca atttcaacgc ggtcaacatt atttactagc gtatttagta
     5641 atcctgaaaa gactaatgca tctttaagtg aataatggtc ttcaagaata tgcggtgctt
     5701 ttttccaacc acccggtttc catttattta aataccatac attccattca tcaaaagaaa
     5761 tcttcattac tttatcagaa cgttttttag ctttcacaaa atcagcagtg cttttaattg
     5821 tatgaatgaa attatccata tcatgatatg aagcaaagaa atcttctaaa ctatcttcgt
     5881 accagtaata acgatgcatg gaaatataat caacatgatc ataaagtgtt tccatcacaa
     5941 tacggtccca atcaggatat gtttcaattt cagcatttga actgccacag gcaattaatt
     6001 taatagaagg atcaacccag cgcataatct ttgctgtttc taatgctttt ttagcataat
     6061 cttcagcatt taggtaacat atttgccacg gcccatccat ttcattgccg agacaccaat
     6121 atttaatatt ataaggttct ttatgtccat ttttaattcg taaatcactg taatatgttc
     6181 cttttggaaa attacaatat tcaacaatgt taccagcgtc ctttggagtg cctgttccta
     6241 aattgactgc catcattggt tcaatttccg cctttttaca ccagcgcata aattcatcag
     6301 tgccgaatgt gttatcttca atagtttgcc aagcgtaatc taggcgaacc ggacgatttt
     6361 ctttcggacc aatcccgtct tcccaattat atccagaaag aaagttacca ccaggataac
     6421 gcaccattgg gactttaagc tctttaatta aatcgataac atcttgacga aagccatctt
     6481 tatcagcttt agggtgacct ggttcataaa tacccgtata cactgctctt cctaaatgtt
     6541 caataaaact cccaaaaaga agattactaa ctttaccaat cgttaggttt ttgtcgataa
     6601 ataattttgt tttcatattg ttactccttc ttattctttc attcccgaga gaacgaatga
     6661 attaataatt tgtttttgga aaatagcaaa aattgcgaga actggaatca ttgaaataat
     6721 tgacgctgcc atatttaaat gaactggttc ttggattggg aagtcactta ctaatccacc
     6781 aataacaacg ggtaaagttg gtgaagccgc tgagaagaaa ttgggggcta agtaatcatt
     6841 ccaaatgcca ataaaccata acactgtttg cgccgccaaa gcgggtttta aaagcggtaa
     6901 aataattatc cagaaaattt gaaaatgatt agcgccatca attttagcac tctcaaccaa
     6961 agcatcggga atatgtgtaa ggaattgccg taagaagaaa atcatcataa tattaccgaa
     7021 caactttgga ataataagtg gtaataaagt tccaatccaa tctaaatctg agaatatgta
     7081 atactgcgga atcataataa caggaaaagg aatcatcata accgcaagca ttaaaataaa
     7141 gattatatta cagaatttaa atttaatttt tgcgaaagca taagccgcta acccagagac
     7201 aaatgtacct actataattg acggaacggc gactataaca ctatttaaaa gacccttcca
     7261 cactggaatt gttttaaaca cttcgatata gttagagaag ttccattcat gaaataaagg
     7321 accataaaca gaaaattggt taacttttaa tgacgctgat agcatccaaa taagtggcgc
     7381 taacattaac actgatccaa gtaccaaaat tacataagtg ctaatatcgg aaaagagttt
     7441 ggcacgctta attgaacgat ttgctttgat attcgacttt ttcatgcttt ttccctccga
     7501 taattcgtat aaaactgaat agcagtaata ataaagacaa acacacctag cacccacgcg
     7561 acaactgaag cttcgctaat acggtgggca ccaaaacgct caaataagta aattaagaga
     7621 gtcgtggttg ctctacttgg cccgccacct aaaagtaatt ctggctcaac ataaagctgc
     7681 attcccccta taataccggt cactacaata aagaaatgcg tcgggcctaa taaagggaaa
     7741 gtaacatgcc aaaatatttg ccagtcatta gcaccatcaa ttttagcggc ttctttataa
     7801 gtatcagaaa tgccttgtaa acccgctaaa taaagtaaga ttgatgttcc tagacccttc
     7861 catacaagca tcataataag cgagaaacgg gcccatcctg gttcaccaag gaagtttatt
     7921 gcttttacac caaagagagc taaaaaatca ttaattggtc cttgggtatt catcattgct
     7981 cgccataata taataattgc agtaattgag gaaacaactg gaatatagaa aattacacgg
     8041 aaaatgccgg taaatttaag tccgcggttt aacaacattg caattgcaat tgaaagaatc
     8101 atcccaattg gaatcccaat aagggtaata agggtattac caaaggcttg ccaaaataat
     8161 gaatcactta acactcgccg atacatctca aatccaactg tcggcgcaac actaagggga
     8221 acaagtccat tccagttttt aaaagttgaa accaatgaat agaaaaaagg atatacgata
     8281 aagaaagcaa tcccaatgaa aactggcaaa ataaataaat atcctattat gttttcttga
     8341 acatgtttaa cttttctttt ttttggtttt tctacaatat ttgtgttcat aatatattac
     8401 tcatctaatc tatgcccgaa catcgagagg atcgatgtcc gagcaatgat ataaataaat
     8461 tacacaccag ccgcttcatc ggctagtgca atattaagtt gagcctgcat tatcggctgc
     8521 acataagcag cgtactgtgc tggcgacatt tcaccacttg ggtcatcatc agtaactttc
     8581 caaacgcgtg ataattcagc gataaaagcg tcataccata atgagttata agccatggct
     8641 gttggatgga gtttcccgtg tcctttttga ataacatcaa ggaaaattcc tttgttttca
     8701 ggtttaattg tttcatcact aagatactca tcaaccatta gcttgttatt tggtaattga
     8761 attgtttgat atttagtcat acgattatac gattcggtgc tcatactcat gtaaagcgct
     8821 agtttaactg cttcttgcgc aactcttgtt cgtgaagaaa cagcgtaacc taatgtacca
     8881 atatatgtat accactctgg actagcatta tcttttcgtg gtgttggcat taaaccataa
     8941 tctaataatt cttttggtac actttcaaat gcggcaacat cccatacacc aacattaaag
     9001 aaggctactt cacccgccat ccagcgatca tagtgtggtt taacccccat ttgttcgcgt
     9061 gtttgagaaa taccgcggtt atccattaaa tcaatgaatt cagtaaaacc agcgataaat
     9121 tctggagtat tgacagtaac cgtcttacca tcatctgata accagttacc acctgctgcc
     9181 caaatccatg aatgcattgc ccatgtatca attagcgcag taccaaaata ttcaatctcg
     9241 ttatgaataa actttgtagc gcgacatgca tttgtaaatt cttcaaatgt atatacgatt
     9301 tgtttgtcag tacgcggatc attttcccac ggcaaaggca tcccgccttc acgacttgcc
     9361 catgctcctt ttttcgtatg tgttgcccag tgaccaagct caaccgacag attaataata
     9421 ttacgattgt agcctaaagg ataagaggaa acatcttttg ggattgccca tacggcttcc
     9481 ccttccccac gctttttggt tgtcacatca taacgatagt aatcaacgac tccatctaag
     9541 aaatttttat aatcaagccc atcctccact tctgcttcta tttctaaata aggtttaaga
     9601 tcgagaataa cgccattcat cgcataacga ccaattgacg ctgggtttga ataaaagaca
     9661 tcaggcgcgt tttttgttcc taaagatgca gttaatgtct cagcataccc atccgtgtta
     9721 acgagttcag cattgacttt aattcccgtc tcttcagtaa acttttcaat aatctttgaa
     9781 taaatacttc gctcatgagc ttggccataa ataaagatag taacactatc acttgtttta
     9841 cctttacaac cagttaaaag cagtggaatc gatagtaata atggcatcca tttcttttta
     9901 aacatattat tccctccttt aaacgggttt tgaaatgcgt ggataatctt cagggtagtc
     9961 ccagtttaca tctggatgca agtctaagta ttcttgtgga agcatattat tttgttcatg
    10021 actgccaaca gcgtgaagaa tttttatata atcggagtca acattagtaa caccgagttc
    10081 agagaaggga atgtaaattt caaaactcca tccttcatca gtatcttcgt tattatcaat
    10141 tgtgccgcta atcacaaatt tatattcagc atatggcgca aaagatgatg ctagaccccc
    10201 agcgccagtt tcgtgccatt gtggtcccca tacatcaaag agccatttaa gtgaattaag
    10261 tggtaaaccg tccttattat aaacatgtat acgcatttcc ataccagcat ttttataagc
    10321 ggctccagta ccatttggtg cttcaatatg tggatcttca acttcaacac caagataaat
    10381 acctttttca ccccaaattg ctaatgtgcg ataatcacca gcggtttcat caggaacgcg
    10441 ttccttaaaa ttgccagagt tagtgacaaa agctgtgtcc cataatgttt catcgaggac
    10501 gccatcaatt gttatttctg gaggatctgc tggaaggtta ctacctttac gattaccaag
    10561 ataaacaagg cgattgtaca ttgccggcgc aagaatgttt gcatcagcgt gaatatcaac
    10621 acccatatca cggtgttcaa tataatacat taaacaatta gcgtcttttg ctgcttcttc
    10681 agttgtgcct aaatgttccc aggtaaagaa tatttccaca acataatact gatctttaac
    10741 gatattaaca gcaattgtcg gaatttcttc tccacttata acgccttcat aaactccgct
    10801 atcaacatca acccatgcac catattcatt acctgtagtg taagtataac ggcccatcgt
    10861 atcgacgcgg aacaactttg ttgattcaga cacaccatca tcactctttg ggacagcaag
    10921 ggcatcaacc gcaagaccaa catagtcact agcaacaatc ccacttggtg cagaaacttg
    10981 atgttttgtg tctgtcacag ttaagccaaa atagacaccc tctctaccaa ctgcatactg
    11041 cactaatact cctgctttaa cacccgctgt gcgcccagga atgacattat aagcttcttc
    11101 atttattacg ccatctactg tcatatttgt taattcgggt aatgtttctt cttcagaaac
    11161 tttaccacct ttacaaccac ttaatacaag actagcagag agcaatataa gtaaataacg
    11221 tagttttttc atttaaaact cctttactga aagttcttcc agcgagggga tcactggaag
    11281 aacctcactt ttatttaaaa ttattctaat gtaaatactg ttccactcac agctagatct
    11341 tcaacgataa aatcattgtc gattgagaat ggaaggaatc cagttaatag accagcagtg
    11401 ccaccagcgg tattaacttg gttaactaat gttgcggtag tattattaac aaatgtttca
    11461 ccatcggttg aagtaaagag tttgaaaaca acagtgtaat caccttcacc gactttagta
    11521 acttgacggg tgaatttaat ccaaactggt ccttctttta atggaatacc agttgcttca
    11581 ccttcatctt ggaattctga agcttgttga gcatcacctt gaataccaat tgcattacga
    11641 tttgttaata gacggagata gtcacctgca aaattatcag cacctacttc accggtccag
    11701 aaactaaata ataatttcca aacctcatta agtgtggtgt atttgaaaga aatggtgaaa
    11761 tcttgtaatg cttcaccacc ttcaaagcgg gcaaatggaa taaatgctgt tgcatagtta
    11821 ccagtataat cttcgcgatc aaatacggta aagtttttgc caacaacgac ccattcattg
    11881 aagacacctt cactagtgcg atagtcaagg tttgtataaa tgttatctgg ataaatcgca
    11941 gcaatagccg ctttaattgc atcaacatca cctgataaat caccggttga aacaacaatt
    12001 tctttagtag cagttgcttt taatcctgcg ctatcttcaa ctgtcagtgt taccgtatat
    12061 gttcccggtt ccattggtcg taaataataa acactaagtt tttccactaa accaattact
    12121 ggatcaaaga gaacacctga tggtttatta acagtaaatg ccactttgtc aaaagcgaca
    12181 gcgacatcgt cttcagttgc gtcgtaataa agcgcaacac caacgcccgc gacacctgtt
    12241 gcggaagttc cgttgaacgg gtcagcacca aatttaatgt tagcaactgg cgtttcttcc
    12301 gaacttgctg gtggttcttc acttgaaacc ggtggttgtt ctgtagtaac gcttgttact
    12361 ggtggctgtt ccgaacttac tggtttttct tcacttgatg ttgtcttaca tcctgtaagc
    12421 agaagtgcta aaccaataag tggaataagt agtcttcttt ttttcatatt attctccttt
    12481 tttattagcc ttttttggct ttataattat ctaaaatgtt cgttggccca taaacttagg
    12541 ccattgcggc tataagccgt aattgaaagt ccggctcgtt gaagatcgtt attccattgt
    12601 ggaataatga caccttcata tgtatttaag ccaattttaa tcgtgacata ataatcttcg
    12661 ttaatgctcc atgtaccgat agcatcttcc tcagcatcta gcaagtcacc gttttggcta
    12721 aacttataag ttactgatgt agcaaaccct tcactatcac cccctgtatg tattaataca
    12781 tcataatctt ttgacgcttg tgccattgta ataacttgaa gttcttcgcc cgcatagcga
    12841 tttggtgcaa gtaccggcca accatttttg ttaaaaaaca tttgattgac aaattgatta
    12901 tgataacccg tcacacctga tgcgccacta cgataacgcg aatgataaac aacataatat
    12961 tttccgtctt ctcttaaaat cgaattatga ccgagtgcag cataacctat tggataacct
    13021 tgaaattgat aattaccagc gagttttaga cctgaattac gataattttc agcactcata
    13081 ccgcgcgggt cagtataagg accgtctggg ttcttactgc gcgcaacacg catattataa
    13141 ttagtagata aggaaccatg cgatgccatt aaatagaaat aatcagtgtt aggattatag
    13201 aaaatgaaag gaccttctgg gccttgatca ccaccttgcc ataaaagctt gccgtagcca
    13261 tcttcttttg gtaacccaat attttcgccg cttgcatata attctttaat ataaataccc
    13321 gcaaaaaatg aaccataaac taaccataat cttccgtctt tactaaagaa taattcagga
    13381 tcaattgcat ttggtccacc gcgaccatca gattttacaa tttccacaga attgtcgtat
    13441 ggaccaagca cactatctcc ttcaacacga ccaatataag aaacactact accaaaagtt
    13501 gataaagaat agtacatata atatttttca ccaaacttat gaacacaagg tgcccaagta
    13561 ctaccgggtc tttcaccaac atgagcaaaa gcgtccgcta atacagtgcg ccagttacga
    13621 gcatataaaa cattagcgcc accatcatta gcaacttggg tccattgcat taagtttatt
    13681 gatttagtaa cagcaaaatg agaaccaaaa gtataatagg tcccatcatc atccttaaag
    13741 attgaaggat catgtgccgt aacattatta ccaaaggcag gattagacgg ataaactact
    13801 tcattattat taaattcact actccgtatt tcgctattca cgcttgtacc tcccttacac
    13861 gatgttaaaa ctgttgtcgc tattaaaaga gttatcgata ggaaaatacc tttacgaaaa
    13921 aacttctcat tattactagc gctagtttta tggtaacaaa acatagattc ctccttaggt
    13981 tcatctaatc gttatgaaat atgtggtaat tatcttacct acagattatc acatgtttac
    14041 taaatgtcaa gcgtaattta tttctggcta aaacgaaaat ttgcgttgta atttctttgt
    14101 tttttaacat agttcatata aaatcaatgt tatttgttgc gcagtttaaa attgtttact
    14161 gtttgtttag taaatatttt aatttaattg ttgatattta ccagacctta taataaaatg
    14221 aaactaatta atatgaaatg aggtgcttat gccaaccatt cgtgatattg ctagaaaagc
    14281 taatgtttca cccgcaactg tttctcgtgt cttaaacggc gacccaacta tcaatgttca
    14341 agctaaaaca aaaagaagaa tttttgaagt tgccgaagac ttagcctaca ctaaacattt
    14401 gcaaccaaag aaaaatcaac aaaaaaataa attccgtatt gctatttatc actggtatac
    14461 aatcgaacaa gaaattaatg atccttattt tatttcaatt agaattggcg tccaacgagc
    14521 atgtcgtgaa cttggcattg cttttgatgt aatttatcgt gaagatcgca tcacagatcc
    14581 ttttgaagat cagaaatacg atggtgtgat tatgattggt agttttacga gagagacaca
    14641 aacatttatt tctgatcgct tttcggctgt tgtgtacgct aattcggaag acgaggatgg
    14701 taaatgcgat tgtgttcgcg ttgatttccg cgcactaaca cacgatgttc ttcatttact
    14761 aatcaataaa ggtcataaga aaattgctta tattggtggc cgcgagcgcg tttcttctga
    14821 accaaacaaa acacctgatc cgcgtgagtt agaatatatt gaagtaatgc atcaaaacaa
    14881 gttatattat aaaccttatg ttaaggttgg tgaatatagc gctcatagtg gttatgaact
    14941 cgcaaacgaa ctaattgaca ataatgaagg caacacacca acagcgatat tctgcggcaa
    15001 tgactcgatt gctttaggtg tttcaaaagc tatccaggaa cgcggtttta aaataccaga
    15061 cgatattgct atatttggtg ttaatgatat tcctaccctg caatttactt ctccttcatt
    15121 atcatcagtt aaaatccaca ctgaatttat gggcgaatta gcgcttaaac tactaaacga
    15181 acgcatgaaa aaagaacgtt cactaaaatt aagtgtaaac gttccttatg aactcatttt
    15241 acgcgaaagt gcttaaatta atttatttca tttgttaagg tatcttctgt tgatgccttt
    15301 ttttcttcac ttttaactac cttcggatta ggaataacta ggttaagtaa taccgctaca
    15361 agcgtggcaa ttgctaaggg agataattca acatttccaa tcattacttt aagtaaacgc
    15421 gggtcaaaag ttgttacacc aaccgcgcta aggatatcac ttgcgacact aagcgcacct
    15481 agtcctaacc caaccgctaa agtaacactt acaacgatta agtcttttgt ttggccaata
    15541 tcagctttat gttcaatgac catccgtaaa cctgaagcac taatcattcc aaagagaata
    15601 attgatgccc caccaataac cgcacttgga atagtagcga ttacattacc aaaaatgcta
    15661 atcacaccaa agataaccgc taaaattgca gcataaaaca cattacgcgg ttcatagttt
    15721 tccgtaatta ctaaaatagc agtattttca ccatatgttg tttgcggtgg tccgcctaaa
    15781 gcactagcgg ccataatcgc aaaaccgtcg cctgctaaag tgcaatcaat accaggatca
    15841 acaaaaaagt ctttgccgca tactgtacta ttagcgctta tgtctccaat atgttccatc
    15901 agtgttacaa aagcaattgg aaccatcata agaatagcgt tccaatcaaa ctttaaatat
    15961 ttataaaagc ctagaagcga accaatactt tgaaatatga caatatcact acctttgagt
    16021 gtgctaaagt cgactaaccc aactattgct ccgtatacat aaccaatcaa aaagccaagt
    16081 aaaattggcg ctgctttaaa aaatgatttg ggcttagcaa aagcgctaat ggcaataata
    16141 cttaagagtg taataagcgc agtcgtccac accttccacg cctcagcgcc ataaagcgca
    16201 tatggggcaa tgatattatt ccaaaagatt gaaggtacaa gtaacatccc taacacaata
    16261 atcacaggac cgacgacacg cggcggaaac atttttcgca ccttttcaac accaattagg
    16321 cgaattactc cggcaaaaat gaggtacatt acaccagtaa agaaaatagc aattactagt
    16381 ttgcccatat tttgttgcca cgcaagactc cccacttcgc cactttccgc cataatactc
    16441 atcagtgctg gcaagtaagc gaaacttgaa ccaagaaata ctggtacttt cctttttgtc
    16501 acaaaataga aaatgattgt cccaatccct gcggtaataa gggtaataga aattgataga
    16561 cccgctagca ttggcacaag cacagtcgca ccgagcatcg caaacatatg ctgaaaactt
    16621 aggactgttg ttttcataaa ccctttgaga cgggttttct tattagtttc cttcataata
    16681 atttcttcct tttagttatt aatcattatt ctaccattta ttcatttagt gaatctaagt
    16741 atgttactaa cctacttgcg tgactttcat tatattcatc acttttaata aatgctttta
    16801 attcttttag tgttaaccat ttatgattaa ttgtttcttc tttttgatat ttaattgttt
    16861 tatctttcgc gcttataatc gctaaatacc cttgattaat tgctggtaca tctttttgga
    16921 taaagtaata agttggtgtt acttcgttaa tgactaatcc tgattcttca aatacttcac
    16981 ggattgctcc ttcttctttt gtttcaccag ccttaattga accgccacag cttccttcat
    17041 atttcccggg atattctttt ttatcatagg agcgttgcat cactaaatat gtaccatctg
    17101 tgtgcttaat taatgtttca acaataatat gataatagcc ttcaggtatt aatgcgcctt
    17161 tatcaacaat tttccctgtt ttcttttcat cctttgtata taaatcccat ttttccatga
    17221 cataacacct ttaattattc gtaattaaaa ctattgtatc aaaatatatt tattttctta
    17281 aagactaatc taagtgaatc gtgtttcact aaatattttt ataattataa aatcaaaaga
    17341 aagagccgca tttgcggctc cctccattta gcttatttta gaatttattc aaatattatg
    17401 ccttcttcat aatcactaaa gtctggtaat gtgatagtga actcttcatt gaattttggt
    17461 ttcatatcaa ggaaaccact caatttgcca acttgtgtat atcctttcgg atatgtttca
    17521 cttggttcat aaaccattgt tgtaattaat tcaaagtccg cgcgcacttg cgttaagtga
    17581 tcattaccat ctgcgccaat tgtaatcttg tactctctga gatcgagcac ttcttctagg
    17641 agcgcaacat accactgcag aaattcttcg gcttctgccc gttcttcact tgtcatttct
    17701 tcactaaaga gaattccgcg taagccgctt tccatcatcg caataaacgc atctttgttt
    17761 atgctataga tgacagtgat atttctccca tttttcgtcg cagtaatatt tgctgtttgt
    17821 tgtaaaacat ctaattctga gccaataaac attgtcggca ctggtgcgtc acctaaatca
    17881 aacccttcaa gtccttccgc tagttgttgg ccatttgttg gacccatatg ttgttttagt
    17941 tttacttcag tgtcaataaa cggttcttct gggttaccat aacgcttacg cgtagcatta
    18001 tgataataaa tccattcact agcgtaataa aactcacgat tatcaataag cttatatgtt
    18061 tcaacaccgt cttcagtcat gtcttcaagt ggttcacccg ttaccttcgc tactggatca
    18121 tcagtttgta gtcctgttgc tttaactacc acattagagt tcgtattttc aactacatga
    18181 tacttaagag cgtcgttaac atattcttta gtatcatcag ttacttcttg taatgttgtt
    18241 tgctgatatg ttgtagcatc agcgatatct gaattagtaa atgccgcggt aacacggcta
    18301 ttagcttcgt ctctactgac ggctccacct gtttgtgagg ttgtgcctcc gcctcctcct
    18361 tctgaatctt ttgatgttgg tccaaaatca aaatcgaccg aacatccgcc aagaagtagg
    18421 gctaaaagtg gtagccataa cattcttttt cttttcatag caaagtacct acttacctat
    18481 tgctacacct tcattttaac attcaaatac gcgtaagtat atacactttt ttgttgttta
    18541 atggtaaaac agcggcaaaa cacggttatt tcactaatta taaattacac aatataatgt
    18601 gtaaaatcgt aataactggc attaggagac tcactttttt acctttacaa cacactactt
    18661 ttttcatcag tgtggcaatt taaaaaagag tcgccgcagc gactcttgtc attaatagca
    18721 ataattgttt tatcttatta ttggttgcgc ttctggataa tcatcaatat ttggatactt
    18781 aattgtaagc tttttattaa tttccatagt tgattcagta aatgtcttta attcttcgct
    18841 agcaactaat acttttggtt cgacttcttc gttatatttg ttagtaacca tatcaatatc
    18901 agtccgtatt ttttcaacat aaccatcttt agaaatacca agggtcattt ggtatttatt
    18961 aattgacata actttactca tttcttcaac cattgtatta agttcagtgt caaatgcttc
    19021 acgttcttct tcagtcattg catctaaatc tagaccaaag gctaataaca taataagcac
    19081 gagtgattta acatctaact tataggtaat tgtaagtttg ccaaaaagtt ctttggcttc
    19141 aatttctgaa gcaacctgaa agagtatagc aagtccttct tcattaggag caccaaattg
    19201 atattcacct gccgagaagc cagataagta atcaaacacg gaaacaactt taaagtcaat
    19261 tggaactatt ttaacttttc ctttgttttc ttcagtttcg gtttcgcctc tttcacgaac
    19321 agtcgtagag aaaccgccaa agagccaact atcctttaaa taaaatgctt cttcatggtt
    19381 tacataatat gtttcatcgc catcaacttt ttcttccatc atttctttgt aagttgcggc
    19441 aaattctggt gtatcaccat gtaagccttt tgctttaaat gacgattcaa attttgaagt
    19501 aattgtttca gttacaacag tttcaccttc ataactttta attgtgtctg aaatttcgct
    19561 tttgctcgta atcgcttgcg tatcaatttc tgcttcttca tttgctttag tcgcggcaag
    19621 ggcgcgttct tgcgcaacgc ttggttcgac tttcttacca agtgcacaac tgtttagtaa
    19681 cattgccgca attgggagcc ataataaatt tcttagtttc atagtaaaag tatcctcctt
    19741 tctactattc atacattata gcattgtatt tttcttttta ttgtaaagaa acatataaaa
    19801 ataacaattt tgattaataa aaaagtcgca cagcggcgac tcttctaatg acattattta
    19861 tttacatcaa tgaccgttca acataatcac taaaatctgg aaacttaatt gctacttttt
    19921 cattaagttt tgtattaatc tcggtaaatc cttttgattt cactactatg acatattcag
    19981 gatcttcacc cataccttgc ggatcagtta tagtttgtgt tacatcattt tcaacacgta
    20041 ctttagtaat ataaccttct ttagcaattt taacttcgat tacgtacttg cgaacttcat
    20101 aacctttttc aagttgagca attatctcgc taattatggc atctactaat tcacgttctt
    20161 cactcgttaa atcaccataa tcaatacccg ccatctgtgc agcaacaaat cgcacaagat
    20221 catctttgga taaattatat gtaatggtta aaacaccagc aaattcacga gccttaacat
    20281 ttccagtaat acttgctaaa agtaaaattt gactataatc aactggacca aaattaaccg
    20341 ctgtgcctga tagttcacta aacacagcaa ggacattttt attagcaaaa tcaatgttac
    20401 caacatttat ttttattttg cctttcccgc tctttggcgc tagtgaaata ttaccacgct
    20461 ctttattatc ataatcatta taaaaccatc catcaacata gtacatattt tcattaactg
    20521 atacaaacga atcttcttgc ccatttttat caattttagc tgttgctttt gcacttgcta
    20581 caacttgagg attttcccct aataagtctt ttgctttaag tgttgttgta cctttattag
    20641 taacttttga tgttgtttct gcacctaggt tagtttgtga aacactttca gtcatatctt
    20701 gctttgcttc ttgaaagaga ctgtctacat ccatttcttc ttgtgtttgt attgcttctg
    20761 acacacgctc atttgcgaca tctttatcaa cttttttacc aattgcgcag ccattaagta
    20821 ataatgccgc taacgggagc aataataact ttttaaattt cataaagaat gtttcctttc
    20881 atgtattagt ttaattatag cacttattta tttatgattt cggggaccaa ttctgaaagt
    20941 attatttggt aaagcgtatt cttacatgtt tcataagttg ttttttgaaa caacagtttt
    21001 gttgtcactt tattaaaatc tgttttatat acatcggaat caattatatt cattaatagc
    21061 tggcgtggtt tttgaccatc gctaacatat ggattgtatt cagggtattt ttgtctttcc
    21121 gctattacat tatcaataag tgatggaagt attttttttt aataatccac tttcccatat
    21181 catatgaaga tcatagagat gtcttgaatt tcgctcatat ttattaagta aatgataatc
    21241 acataacgca aatattttat caacaaaagt tctttcaata gattgcgtta aaaataaaaa
    21301 agaatcaagt tgatattgtc caattatatc gttttttttc tgaattttaa ggtattctaa
    21361 aattaagttt tctgtttttt ggtaaatcgc aggaaatgga ttataggcaa tcattgtttc
    21421 aataataagt ttttgaagtg tatcgacatc actgttaaag acactgttaa attttagaat
    21481 gtagcgatta aaatgttttc ttgattcaat ttcatttatg tttattactt ctgcaccaat
    21541 ttcttttgct gtttctatta ttaattgttt aagatccttt ttttgttttc tggttagttg
    21601 tttgttttga attacagaaa ggtcaatatc ttcagaaaaa cgactaataa ctccataaca
    21661 tttagataaa gaggtaccac ctttaaaaac tatagggatg tttttactca tattaccaag
    21721 cggttttaaa aataatgata tgtaataatc tttttctatt tgatattctt ggagcccata
    21781 atgagtagcg gttagaataa caatttttct aaactcttta atattttgat gtaatagcat
    21841 cctgacacct aattttataa aaattaactt gtgcttcacg cgggtagcta ttaactattt
    21901 tttctacttc ttttctttct aaattaacac cttctaaata tttaaaaatg gtgcttgccc
    21961 cttcttctaa atttatctcg ctaaaactag tgtaatcatt taaaaggtca aggatctgta
    22021 ataatttata attgtttgct gtgacttcta cacgcggact attaacaata acacgtgttt
    22081 tatttaattt cacttcacgc tttttattag cgacacaatt tgagattatt gaatcaactc
    22141 ctgcagtttg cgttgttaac cctaatttgt tagcgaaact tatgcccgta cgataaccga
    22201 atatctttcc atcgtttcga atatattttt tattaataat ttcattattg gaaatagcta
    22261 tggtaccgat agcacaatca taatttggta aagagtaaat accttctgca attctaacga
    22321 tattattttc ctttactaaa cgacttaaaa tagagcggat tgtacttcta ctaatttcac
    22381 tatatttttg atatatatct ttagtaaaaa ttggtgtctc tttatcatag ttattaaata
    22441 tgtaatctat aattaaatta ttaatctttt tttgtttcat cgctattctg cgtctacatt
    22501 ttatcatctc tactaagctt tgcaacactt tttcttatta atgtttactt gctgttgcat
    22561 ttaaaatcaa ttttaaataa taaagtattg atataattaa cattatacgg aggtaacatc
    22621 atgaaattag cgactaaacg actaatctta agacctttta cactaaacga tgttgatgct
    22681 gtgcaaaaat attcaagtaa agaaagtgtt actcgctata tgatgtgggg accaaataaa
    22741 tatgaagaca ccaaacgatt tgtatcttca gttgttagtc gaaatagtga aaccccgcgt
    22801 gatgtttttg attttattgt tacattaaaa gacggcaccg ttattggcgc ttgtggtctt
    22861 tattttgatg aatacccaaa accaccaacg ctagggtggg tatttgatga tacctattgg
    22921 aatcaaggtt atggcacgga aacaggcaga gctctattag cgtttggctt caatacactt
    22981 aaagttaaaa gcattcttgc cacctgtgat aatgaaaata tcgctagtgc ccgcattatg
    23041 gaaaaaatcg gtatgcgcta tgttgaaact agaccagaag aattacctaa actagggaaa
    23101 agatttatta aagtttatga aataaccgcg cttgaatata aattaaatag taacaaggtg
    23161 cttttatgaa attaaggatt agtagtgttt taatactttt aatagcgcta cttctacctt
    23221 tttcgcgaca aaaaccactc gttttagatc ataatgatga tccttttgtt tttcatttac
    23281 aaaaagaatc tttaaaaata gcgcagttta ccgacctcca tctcgcgttc ggatttgacg
    23341 ctaatgatcg gaagacatta aaattaatta gtaatattac cgcggaaaca aaaccagact
    23401 taattgtttt atctggcgac caaacacttt caattactgc tcccgctaga caccgacaat
    23461 taattcgtca tatggagtca cttaaaacac cgtggacttt tgtatttggt aatcatgaag
    23521 cagactatca taaaatggag cggttactaa aagtgatttt tactatgaat acaaagtata
    23581 tgtattttaa agttggtccc cttttagaaa atggtggtta tggtaacttt actattaatt
    23641 acttatatga agataatcct ttttataatc tgtacttact tgattctaaa gcagaactaa
    23701 aaatccctgg ttcaggtaga ctttttgttt atgattactt tagtgaagca caggttgatt
    23761 ggtatcgtaa taaagttatg attgataaaa taaataacat aaaatcgaca gtatttgccc
    23821 atatcccttt aatacaatac gctgacgcat tattaataga aaataaagat aaggttaaag
    23881 gcattattgg cgagcgtatt agtcatcagg aagttgatac aggtttcttt aatgcaatgg
    23941 ttgaaagtac tgttagtgaa gccttctttg ctggacatga tcatcttaat aatttcactt
    24001 ttgaaaaaga tggaattatt ttaggatacg ggcaaataag tggctataat ggctacggca
    24061 cacttagacg cggagcgcgc atcatcgaaa ttgatgagtc taaaatgttt aagacatatg
    24121 ttgtatatga ggatttaagc tatgaatatt aaacgattct taatgctttt aattaacgct
    24181 tttgttctct ttgttatttt atttatgtta acaagaacat tatggacgct cttagtattt
    24241 gtgatgccag ttgtttcact atttttacca aaggagaaaa gtaatggttg agttattctt
    24301 tgggttttta caactatctt tttatatcat tgtctttact tttattcctg ttacactttt
    24361 cgtgcgtgtt ttgtcagtgt ttccaagcaa aaacgatcta aaacacaaaa tactaatcat
    24421 aattgacatc ttttctttaa gttattatta ctttatttca aaagaaaata aatatcgaaa
    24481 actttataat acactgcttt ttatatattt agccttgagc atctttgcct ttggctttgc
    24541 tattcatatg tacatattgt aaagactata agcaattgaa aatcgactgt aattcagtaa
    24601 gtgtttttac ttgaggaata ttgtattttg tgggtggcac aggattttca aaatcgtaat
    24661 atcctttacc gtatttactc gagttcatac cagcgcaata ttcattaccg gcgctcatcc
    24721 aaccaaatga ctcgctccat ccgccttcat aatttaaata ttcaaagaag tcattatagt
    24781 gattaattgt taagaacaca tgtcgatgtt caactggaat atatttagcg ccttcgttcc
    24841 cctcgttatc atatgcagta taaacaaagc gtacagtgct acgtgtaatt ttagtgccgt
    24901 tattatatgg atcgtcactt tcaatcccat atccccacgg cgtataagtg taaccaaaat
    24961 ccatttcgcg ataattatac atatccgctt caaccggtcc accattatca gtgtgcggta
    25021 aagaaggttc atatttatat ttagtaacat cactgctgta ataaccgtta ttaacgcgtc
    25081 catatttacc aaataaggat attgcttgat tcattgcagt actatttttg gccatcattt
    25141 gattcgcagg tgcttcacca aaagcaaata aataagccgc aacatcatca agcgaaacat
    25201 aagcagcccc gtaatagact ttctttactt tgccattaat attgttaatt gtgtatgact
    25261 tatattcacc atctaatgta tattcataca ctccttcatt aatccggtaa ttcgctagag
    25321 tgcgatatac tcgattagga aggtggttaa gcggatagtt tgccgtgttt ggagtgtcaa
    25381 ttatatcgcc agagattaaa cctttttctg tccgaaacat tgcgtcttcg taagatgatg
    25441 ctcttttata attgtttgtg taaaatgttt ctttatcagc gtcacttata atatctatat
    25501 acggattttc tgttggaaca tatagaggca cattataact atcaagagcg tattcatatg
    25561 tttgccaagt aaagtcatca tccactgacc aagtttgtgc tccttctatt tcgctactac
    25621 tttcagttga cggtacatct gtttcacttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25741 nnnnnnnnnt gtttcactta gggagttatc agaaggcttt aatacttcac ttaaacttaa
    25801 aggcgtacag ccgcttaaaa gcatagttaa taatggtaat aatattaatt gacatttttt
    25861 catgatctaa cctctttatg acaagtctgt catttattat taactaaaac atgttagatt
    25921 acaactttag ttgtagtatg cgactatttt taatcaatct aattgctata atataaacat
    25981 gaactggctt aaaagaaata ttcgttactt tatcttgagc ctatttttat taacaaacgg
    26041 gcttattatt tttgaagcaa cactcgcggg tggtacttct ggagcgcgct caagtttact
    26101 ctcgcttatt ttaagtgtgt ttgttaataa aacagtggac ggtcctgaaa cagcttatgt
    26161 tcctattgaa tcattaacac taaaaactaa agatgacact actttaactg aaggtatgca
    26221 cttctatata ccgctaggca tcactagacg catcactgca actgtattac cagaaaatgc
    26281 tacggataaa ggggtaaatt ggacaagtag tgatagtagt gcgcttgaag tttatccggg
    26341 tggttactta gaagcgcgcg cactaagtga aaatgtatta gtcaccgcta tgactaatag
    26401 tccgctgcat cgcattagtt tttatgtcac tattcacgaa aaagttgcgc cccctgaata
    26461 cgaagtcgct cttactaaag ataggattac tattgacact actacacaac ttgaagttag
    26521 tgttgatgaa agtgtcgcta atgaatatga tgttcgtaaa ctcactttct ttagtgaaga
    26581 cgaaagtgtt gcaactatta ataagtacgg tgttattaaa gggattagcg ttggcgaaac
    26641 ttatgttggt gttgttggtg acgaccgaaa atttaaggtg agtgtgaaga gtaatgatac
    26701 accgctaata tcgccaactt caattacttt agaatttgat gaaatcgctt atgtttacga
    26761 caaaatcccc ctaaattata catttgacgt ggaaaatgtt actgatccat cactaacttt
    26821 tgtatcaagt aatgacattg ttgctactgt tattaaagaa gatgataatt actttattaa
    26881 gacacctaaa ataaaaggta ccgctacaat tactgcttac ttaaatagta actttgcaat
    26941 cagggccaca aaagatatta tcgttaataa tgtgatgcca acttctgtta attttactaa
    27001 tgttccaact gaattaatta ctggtcagcg ggtaaatgtc ggttatgact taaatcattc
    27061 cttagcgaaa gaatcgttaa gtgtgactga taagcgtgtt atttttactt caagtgataa
    27121 cagtattgcg cgcgtatcaa ctgggaacat gtttggcact attgttgctt taaaagaagg
    27181 cgttgttact attagcgcga caagtgttgc cgaccctact atcagcgcat cttttagtat
    27241 taaaattatt ccgcgaccct ttattaatga tgataatttt agtgacttcc aaggctttgt
    27301 tcgaaaagca gttggtcact tcttcttatt ttttatcgac gggatctttg gatttttcac
    27361 tttttactta tttttaaaag ataaaaaaag cactaggtta ataacagtgc tttctcttat
    27421 tactggtgtt tttattgctg ctcttagtga atttattcag ctttttgttc caggacgagc
    27481 aggtactatt actgatgtta ttatcgatag tgcagggtac ttatgtgcaa caatgttaat
    27541 gctgttaatc atgtatctaa taaataaaaa taaaagcaat aaaaatgtcc aacaataata
    27601 aaaataagtg ttaaatcacg tttttgaact ttttaaatgg caaaatatca cgtttttgaa
    27661 cttttcgaat atttttatag tgttttaatc acgaatttct tttaataatt cttcgattaa
    27721 tggtgaaaca tcttcatcga caacatcaac gagaaagtca taaatcgatt cacttaattg
    27781 ttccacactt gcagcggtaa agtcgtgttc taaaattaat gtgttatctt cctcttcatc
    27841 taaaacaact ttggcataga gtaatttagt gtttaattta ttaataagcg catataactt
    27901 ttgtggatta ggaatttcgc caatgaaaag tcgcatactc acttgcatga aattagagcg
    27961 gcgataatca ctaatcgtaa tttgataata gaaagcctcg ttataaagac taatcacgac
    28021 tggtacacct aaagtaacac caagtgtttt agcgttaacg tcaagtggta attgaaattc
    28081 aaaatcatta tcaactgaaa tcgcgtgcaa agtcgctgtt acttcatttt caatttcatg
    28141 gtgattaatg ccaatcatga cttactcctt tctttttaaa tactttcttc aagcgaagta
    28201 tttaagaata taaatagttg ttcaactagc tcaccgatgc gtggttgtgc ttcaacacct
    28261 ttacgcacaa aggaaatgag ttgttcttct ttaccaattt cgtaattaat tgagcgaaga
    28321 taaagccacg gtatcccgta gaaccgacaa cacgctacaa tcccgcccat ttccgtatca
    28381 aacactgttg gcccttcaat atagttttct tgacgggcaa taatgcgttc agcttccgcc
    28441 ttttctttca caaagttatt aacactgatg acgggacccc ttactaattt atagttccct
    28501 gttgcactgt ttaatagttc aaagtgttta atatacactt cttcactaac ataatatggg
    28561 tgcatatgaa ttgcgtctct gagcggtaaa tttgtgtaat tgttataatc taagtccgcg
    28621 atataaacgc gttcagcaat aaaaagatcg ccttgattaa gattactact tgcactttgt
    28681 gcactgccaa ttgaaataac aagataagga ttaaatttac gaatcatgta acttgtgagc
    28741 actgccgaca taatattact atgcccagtg taagtcatac atatttcttt accaccatat
    28801 tccccgacaa agtaaaagtg ttcgtcttta ttaaccgcac ctttttcttt cataatcatc
    28861 cggtttttaa tatagagaat gtcatccgga gttgttccca atagtaaaat cattatttcc
    28921 gtcctcgctt tttcttctta ttagcttcga agaggttatc ttcgaggaaa gcttgatatt
    28981 cttcaggtgg tagtggtcta ctatagtaga acccttggat ggagtcagcc cccatctcct
    29041 caagcatctt cacttgtctt tcatcttgta caccttccat aacgacatag gcgtcgagaa
    29101 tgtgcgctaa atcaataatg tttttaatca ttaatcgtga tttttgatca atatcaacgt
    29161 catcaaggaa agccttatca attttaataa catcaaaggg aatcttttta atataactaa
    29221 gcgaagagta accagtccca aagtcgtcaa tggcaatttt aactttcaag tttttaaatt
    29281 tcttcacaat tgagagtaaa agtaatatgt cttgcagtga tggtgattca acgatttcaa
    29341 tttcaagcaa catcgggtta atattatatt tatctgttac tttaattagg tgattaataa
    29401 tattcccagt aaagagtgtt cgttttgaaa tgttgacact gattggtaat aagcgttgtc
    29461 ctttaccccg ccattcatca agttgtttac acactttttc cagcacataa tggtcaattt
    29521 taataattaa gtcactttgt tcggcaaata agataaagtt tgcaggtgag agtagtccta
    29581 actctggatg gcgccatcta acaagtgctt cagcgcccgc aaagcggcct aattttaagt
    29641 catattttgg ttgatagtgc agttcgaatt gttctaattc caaactgcgt tcaatttgta
    29701 aaccaagttg gacatttcgt ttattacgtt catacatctt tggttcataa agtgttaaac
    29761 cacctaaatc actttcggca cgaccgtgat cagctgcaat gagtgcccgt tggagcatcg
    29821 acgccgcggt aataaattcg tcattttcat tttgacagta actcacacca aagtccgcca
    29881 caaattttac ctcattacga ttttcaaata atgttttatt aatttcttca attaagtcat
    29941 tcacatattc ttgtacttgt tcataaggtg tatctcttaa ataaataagg aagttttcat
    30001 tatagtcgta accgtaataa gaatgcgggt attttgtttt aagtgtatta aatgtttcat
    30061 acactaaacc taaaacttcg ccgcctttct catacccata tcgcgtaaag acaatctttt
    30121 taaaacgtgt tgccgcaaaa gcgacaagtg catttgcttt acctctatta cgcttaatat
    30181 ctttagcgac ataggctaaa aacgctcgtt ctgaattaat cccttccact aattcaaatg
    30241 tcgcataatt aaagcgaatt tggtttgagt ttcgcttata atatacaaaa gcaataatcc
    30301 cacatacaat actagcgata cttataatta ctaataaaat gccgattata atatcaagtg
    30361 ttttgttccc tgtcatagga ataagaccga aggacattaa aacataagta ctaaaagcaa
    30421 gcactaaaag cattacacat aagatgctat accataaata cttttttccg ctatgtcttc
    30481 tagctttctt ttgtttcatc ttttatgccc tcctctaatt cttttcgtaa tttttctttt
    30541 tctaattcaa ttaataatcg taatttctct tgttctttaa ttctttcaaa ttcggcctgt
    30601 tgttcactta tgccactcgg cgtggcttca ctcccctcgg tactttttaa ttgttttagg
    30661 aggtccataa gtgaagaacc aaacacaatt aagagtggta ccatcgcaaa cacaagcata
    30721 aatattggat tagcgagcaa cacactaaat ttaccaagta ccatactttg accaacaact
    30781 ttgcctaaaa tatccgtgtc acgcacccag tcaccacgcg cttctaatgt acaaccactt
    30841 tctggacact ctttagcgtg aatattatca ccaagcgtcc ggaagtaaac gcggccatct
    30901 gcttgcacct caatacgatg aatccggtga gtgacaatgc gtttatcacc aggtaaatca
    30961 taacttccgt gataattacg cacaaaagta atgacgtccc catctaatgc tccttcgctt
    31021 gatggtcctt ttagtgtttt tggatcaaca cgttttacaa ataccattgt atcaactttg
    31081 tatgttggtt ccattgaatc agttaaaacg cgtaatgact gataattacc gtaacgcatc
    31141 actccatgat tatttttagc ggttgattga ccaacgactt gtaaaacaat aagtaaaaca
    31201 acgatcgccc ctaaaacaac gcttgtaatt ttaccaatac gccctttttt ctttggcgct
    31261 gcttcactat tttgcttgtg ctttggcatc ttcaatcgcc ttctttcttt cttcagttgc
    31321 ttgtgcggca acagccttac gtgctcggcg taagcgttct gcttccgtca tcattaattt
    31381 ttgatatgct ttccgttcgg caagtaagcg ttctttctcc gcgcgtaagc gtgcccgttc
    31441 catattcttt tcaatttgcc gttgttttgc ttcttctttg cgccgtttag cgcgttcagc
    31501 agcttctaac ttgcgtttaa gcgctctttc ttttgcccgc gctttcttta aggcttcacg
    31561 ttctttttgt ttacgttttg cttcttcgcg tttaagtcta gctttttcgc gagtagcttc
    31621 acgttttagt cgcgctgctt cttgttcagc agtttcaaca cgagcgtcaa cacgcattgg
    31681 taatcctggt ttagcaaaac gttcatcact taattcataa tttaagtcgc ccgcttcaat
    31741 aatcggacga atattctttt gctcaacatt acttaaagca aggcgcgcat gctctgtttc
    31801 aattgtaaag aatgaattta atgaaaattg attaatctta tctaaatatt ttttatcaaa
    31861 ttcaattttg gcttgggttg ttgtatatga aatacctagt tcttcatagc gatgaataaa
    31921 gttccataat ttaaaagcat cttgatacgc ccagtttaaa cgaataatat ttgtttgtgc
    31981 aattggctca gacacaggta cggctttagc catttcacgc ataaagcggc tattaactaa
    32041 gaattgtgtc cgtcttctaa tattttctaa acgttcaagt aaatccatat tactttcgcg
    32101 tgccccttcg tcatcactag gcactgacat tgtaattgtt gcatcaactg tgaagagcga
    32161 atcttcaact tgtacttcac gcttaatcgt aatttggtca ctattacgcg tatcaccatg
    32221 ttctctaata tatttatgcc gcaattcaat aaagatttgt aaccgattaa ttagtgtcat
    32281 aatgaaacgg ttctcataaa tctttaagtc gtcatcaaca taaacgttaa gaatcttttt
    32341 ggggataaca cgaccatcat ctaatacctc agatacatga tcagcatttt gcgccatata
    32401 cttgactgct cgcggggtaa gtttccgcgc tttttctact tgcacgactt cggcaaattc
    32461 attaataaat ttatgcggat taacgacaat ttcttcaaga gaaggaatca cgcgttcaat
    32521 tttttcaata aactctgggt caaaacgttt tacttcatta cgcgtaatac cagacaaagt
    32581 tggtgcttta ttagcaatcg tattgtataa attcgtcgtt ggcttacttt gcttaacagt
    32641 ccgatttaaa aatgaataat aacggctacg aaccttagta aaacgcttgt agccttttaa
    32701 tgcttcacgc tttcttaatt ccatcgccga aagtcgtcga cggcgtggca cagctttttt
    32761 tggtgcttct tttttgggcg cttctttcgg ttctttttct ttagcggcag gtgcttcagt
    32821 atcaagtgct ttttccgcta ccttctcagt ttcaggctta gcgtcagctt ttttagggtc
    32881 agcctttgct tttttcgcct tttcagcttt aggtttagcg tccacttttt ccttttcaac
    32941 tttagtgtca ctttttttac tagcaacttt tttagtttgt tttggtttgg tgtccgctgc
    33001 tttttttgct ttatctttaa ttggcgcttt ttttactttt tcttgggctc cagccacttt
    33061 taaaggtttg tccttttttg ttttaggcgc agtttcttcc ttagcgactt cttccgctac
    33121 aacattttgt tcttgtttaa ttgtaacttt attctcgtct gccattattt acctacgctc
    33181 tcgtcataaa atcatcgatt tgattaagtg aatgtttaaa tgtgttttta ccaaatgtcc
    33241 gttcaataaa ggcgcgtaac gcacttaatt cgtcacgcat aaacgctaag ttaagtgatc
    33301 taaatttctt taaaattttg gatgaaaaca taaagtcaag tgcttctggt tcagtcccgc
    33361 cgctagcgac ataaattggt acaaatgtat gaatttgccg taaaatccgg ttcccaaatc
    33421 caatgcggaa tttattcaca acatattcgt caagcttact aaacttatta gcgtttgctt
    33481 tggaaagtgg gaaattagtt tgtgcttcat caaataaccg ccgtaagtgt tcatacggaa
    33541 tctttaatgg ttcagtaaat tcagcatcaa acatttgtcc acgttcgttt aatgtaatac
    33601 taatcgcacg gtcatagact ttatcggtaa tcgtaaatgt tgagtcgtca ttatttgcgg
    33661 taccaaagaa ccaaatattt tgcccaatta ataacttacc attcactaat tgcactgggt
    33721 tcatttcatc tgctgtcttt gttacttcaa cataccactc ttgtgggtta ggcatttcca
    33781 taatcgataa gaattccgca aagtagtatt caattctcgc taagttcatt tcgtcaagaa
    33841 taacaatgtt tacatcttta cgataacttg cttcgtaaat cgcccgcaag aattcacttt
    33901 cgttaaactt cttcgtaaat tcgttatagt aaccaatgag ttccgtacgg tcacgccagg
    33961 acggttgcac actacaaatc gcgacgtcat ttaagaagaa tttacctaag gagtaaggta
    34021 aagaagtttt accagtacca gagatacctt ctaaaataat aattttaacc gtacccattg
    34081 cggcaaagag cacacgcact aagtcgatat cataaaataa cccaagttgc gatgcggcaa
    34141 aataacgata gcgcactgca atttgttcta aagtaatttc gcggtctaat accggtgtaa
    34201 taaaatcagt gtctttatct ttatatttta agtccactgc cccaagtttt gggaatgtat
    34261 caataacgac cgaagttttg tcaccttcca caagtgaagg atcttcaaga cctaatgatt
    34321 cagctttcag tttaagtact tcatttttct cttgaatggc aaagaagagt tctttgttaa
    34381 gccgttgtac ttcggcaata agtttcgctt tatcataatt tttgcttgaa tacttaacaa
    34441 gtcgacgaat aaggagcact aataaaaaga ttaagccacc aataacggcc acattaagaa
    34501 tcattaagat tacaaagaaa aaccaacccc aaaatccaaa tgagtccatg tacatttcaa
    34561 tgattgtacg ataagtacca aagtcatcgg acactagttt ccatggttca acaaatacag
    34621 cggctaacca ttcgacaaag ttagcccaga atgattcaaa tgtgtctcgg atataactaa
    34681 aaaatgcgtt cataatatca ccatttcctc ttcttattca gcccttttag cgacaaagtg
    34741 taattgcatt ccaaaagact tgtttacaac tttatttgtg gtcgctaagt cccagccttc
    34801 taaccaaaat ttaatattca tttctgccac accaaggtta tttgtgtagc aaagcggttc
    34861 acctttatca ccatcaagca tatatttttg gaaacttgtg taatattggc gctgtggtac
    34921 aacgttaata agcggacgga ctcccttgta agtcaccgca tggaaaacat caccatgttc
    34981 taacttttct tctgcttccg caagtggttc accccagttt tcatttgtga gtggctgttc
    35041 aaattcgcca taagctattt cataaatttg ttcaccactt tcgccatact tcgccccatc
    35101 aagaccaaat gtataatcat aaaatcctgt aagtgacgct ggagcacgcg ggtcatctaa
    35161 gtcaagtctg ccaccaacag caattgaacc tgtataatca ttttcttcta ataaagtgcg
    35221 ccctggacta atgatgtctt ttttagctcc actttggaaa ccaaaacgca atgcttttgg
    35281 taattcattt tgaccatgga ccttcgtgtc tctattgaag taaatggcta tattatcaag
    35341 cgggattgtt ccggctcgat cttcgtcaat taaaagtcta aataatactg taaacccaaa
    35401 ataatctttt ggatctgcta catttaagtt caaattgtgg cgttctgcac ggttttgctt
    35461 cggtgcttca taaagcgtaa gtggtaaata tgggtcttca tctttcgtaa aagaaccaga
    35521 agtaactggg ctaagtctat ttgtatttga atagccctca tgctcaagca caaaactcat
    35581 cgtctgtggc gacacgtttt gcgttgtcca atacacataa ccttcaccac caacccaacc
    35641 ttcatcagac tgtaaattgt atttatgttg ttctgtaact gttagcggtt cactaagtct
    35701 aaacccaact tgcgcaaagc gagcttctgg gtcttttgac aataaggaaa gcgaggttgc
    35761 gtttgcaata tagtaaccaa ttgcataccc taagtatgaa ataatgataa aaacaataat
    35821 gatgaacgct gaaacagcga caactttttt agtgcgtttc cgccgctttt cttcacgaat
    35881 aatgtcgtta gcaacttcaa gaagtggatg ttcacttaaa ttaatttctt ctgccacaat
    35941 attctccgtt ttcttatacc gctaaaacaa tcaatgatgt tttagtgata tatgaacatt
    36001 ctttattatt ttaacacgaa tgtccgtgca cactatccta attttataat ttgatagtgg
    36061 cgattaaata aaaatgatgc taaccactct ttgccagaaa taataacatt catctttatt
    36121 tcttcgaggc aactggctac catcacactt ttaggcccta tagttttgcg tccttagatt
    36181 tctctaagtt tgccttttct gtagttatta tatgttgtac atataaaagt gtcaagaact
    36241 atctaattta agtattaaaa aagccgtatt ttctacggca attttaagta ttttgtgatt
    36301 aattattctt caggtttcgt ttcaggagcg ggttcgtttt gttttgctaa ctctttacgg
    36361 agttcttcaa gcgcttgttt gcgcgcttct tcaagtaatt catcttttgt catttgtttg
    36421 aattctttac ttgaagcttc attcatcact ttaattaaga gataaatatt gaagagaaat
    36481 aacacaataa gcggaaggac aattaggacg aaaaaccata cgcggttacc aattaaccat
    36541 ttaataaatg ggcctcctga tcctgcttga acattcttta aagcgacaat agaaatggtt
    36601 actaaagcga ccatcagcgc taattctaaa ccaagtagca aatatgttgc ccaacttggt
    36661 aatttaaatt tctttttctt ttcttggcca gtcatgaggt actccttcac atgtattatt
    36721 tcacacgtaa taaaatttta cattaaatgt aattaagtgt caaacatatc tatgcgatta
    36781 tatggctctt taacgataaa tctttataaa aatacgtgtt ttttaataaa aaacatcaac
    36841 ccaactggat ggtgtgtttt aataattcat gattaatatt aagagcttat actcctgtcg
    36901 agtaaatatc aagtttaatt ttaaacttac cgccaataat gctttcagca gcatgaatat
    36961 cagtaccttc aatccacaca cggaatgtca catcagcggt accagtacct gctggtaaca
    37021 ctgcaatttc tggataaact gcttgtgaat ctgcaatagt gtagtgtgtc tcaaacgaat
    37081 cactttttac tgttgcgggc actaattcat gggtagtaag accatacgct cccccacttc
    37141 ctaattgaag atattcggga tttatttctg cttctggtgc atataagata tcaacacttg
    37201 gcgtgcctgt agctgagtca ttgttatcat aacttattac cgccatgcgt aatgctttta
    37261 atgcaccaac atcttttgga ttagccggat ccgccggcag aatcgctgtg ttttgcccaa
    37321 gaaagacata taaatttaat gggtcttcgc caggcgagcg agataatgta agagtgaaat
    37381 caataaaata tccatccgca tcgccaggat ttgtacttgg tacggtatta atgactgctg
    37441 cggttacatt gtctgaattc cagcgcggtt tatagaagtt gataccattg cccgagatat
    37501 ctgtaacttt attcgtgccg gttaatgtta ttttagtgat tccgtctagt tcactagtag
    37561 cggataatga attcaaagag ctcttatagg taaccactaa atcagaatcc cgcatgtaaa
    37621 cttcagcatc accataagtg atacctgcag tacgcacagt tgtaaaccac gctaaggttc
    37681 cactagttgc ggcaatagcg ctaataaata atagcgttaa tactgttcct aatcttttat
    37741 ttcttttatt cattagtttt atttccttaa ttttttattg aggtttgtaa aaagtttcac
    37801 cccagagaga atgtccctgg ggcgaaacgg ggtcgcaact ttaattagtt aatgaatgtg
    37861 tataaagcaa tgttaacatt gaacacacca ccgagaatgt cgttattaca gtctttatct
    37921 tcaccttcta accaaatacg gaaggtaacg tttgcttcgg tagcaacacc tttacttaag
    37981 tcagcaacta agaaaccagc cagatcagca gcggccttgc tgtctgcaga agtgatttct
    38041 ccgtgtttat agttaagatc agttgtagtt tcgaaacctg acacaccgta aagtgagtca
    38101 gtaccagtag taatgtattt tggttctgtt tcttgttctg gagcccagcg aactttaacg
    38161 tctccttcat gatcaatgac tgctaaacga gcagctttga ctgcaccagt atctgcaaga
    38221 ttttcaggat cttttggcgc aatagtggta ccagtaccaa ggtaaacttt taaaccgttt
    38281 tcatcaacat cattatcacg gcttaagcgg acggtgaagt caatgtagaa tccatcagca
    38341 acaccaggag aaccagtggt tggaactgtt tcgatgcttg aagcagtact aactggggtt
    38401 gtccatactg gtttatagaa tgttttacca tcaccagaaa tgtcagtaac tttgttagca
    38461 ccagcaattg taactttatt ggcttcagca tcataagttg aaccaccact ccaattattt
    38521 aatgagctta ggtattcaat ggctaagttg ccatctgaag cttgaactgt agcttcgctg
    38581 taagagatgc tagcggtacg cacggttgta aaccatgcaa aggttgagct tgtcgcggcg
    38641 attccactta agagaactaa gccgatcgct cctaataata ctttattctt tttgttcata
    38701 atatgtaata tcctcctttg aacaattgcc ggaagttgtg gccggcccca ctttcatcga
    38761 tgagatgaac taattgggca attaggttct aatttaatat ctttgatatt agtgtagtac
    38821 ttgcctagag actatagatg tctagggcaa ctttgaacat gcctccgagt gccttgtctt
    38881 tggtcgcttc cgcgtccgta ccttcaatcc agaagcgaaa cccaacgtat gcttcggagt
    38941 cactcgataa atcggcaatt tgatgagtgg gtccatccgc ttcactaagt tttgtaacga
    39001 atggatcact gttaacttct gcacttgcgt catgaacttt atagccatta acactgtagg
    39061 cggtgctagc cgtatcttta ataatatatt ctgggctagt ttcgacgaca ggggcgtgca
    39121 gttcaattag tgcaggcgaa gcgcggaatt cgtccgcata acttattact gccatgcgta
    39181 ctgcgcttac tgcatcaaca tcgcgtggtt tagtggcatc gaccggaaga atcttcgtat
    39241 tttcgcctaa gtagacttta attcccgttg cgcttgggga atcgctttgt ctagcaatct
    39301 taaggacgaa gtcgatgtag taattttcgg cagtcgctac ttctttaata tttgtagcaa
    39361 cgccttcaat tggcgaccac acgggtttgt aaaaatctaa accatttccg gaaatatctg
    39421 tgacacgatt cgcacccgtt aatttaattt cgtttgcttc gtcactaatc gttccgaaag
    39481 tgtttagtga atcaacgtaa gtcacaacga gattactatc gctacataca acagtcgcat
    39541 cactaaaagt aagtgaagca gtacgtgtcg tggtaaacca cgcgaatgtt gtacttgctg
    39601 cggaaagacc actaagtaaa acaagcgcta gagcaccaaa cataattgat tttttgtttt
    39661 tcatatgttc atcttccttt cgttgcgctt gtcgtgtgtc ttacaaggtt tgaccgagtc
    39721 ggaagagtaa gttctttgta atgtattact agttttaagt ccgagtaata aaaacagcca
    39781 cctattctta cgctcggtag ctggctatct tattgctaag accctatagt tttgcgtcgc
    39841 tagatcgctc tagctttgcc ttttacacca tgattatata taattcagaa attttgtcaa
    39901 caaggaatat aaaatttttt aacttttttt cgtttttcca tttattttta tcattctatg
    39961 gtggtttaaa aggttgttta tatattttat ttacatttgt tatcgttatc tttcttattt
    40021 ttaaagtaaa attttacaat tgaaattttt gttagatttt tcgagaacaa ttttaaattt
    40081 aatgcattct atgacgaaaa taaaagcact tacatcagta aatttaagtt taaaactttt
    40141 caaaaattat tatatgtaga tataatacaa cacactggaa tatatctatg gagaatttct
    40201 ttgttattgt tagcgcattc ttctacctgt caatcatgac agcgtctttt atatttttaa
    40261 atcggcaaga tgatcaaaat tcacgtcgct ttcgggttgt cgtcttactt tatgtgttta
    40321 caatcttagg aataattctt gatacacttt atcgtttaga ttatggggga ctaattaatc
    40381 ttccgtcata cgcctttgct ggagttatta ccgctacata tgtttgctta ccagtcatgt
    40441 ggggattatg gtgcttatat attagtcaca cttcatattt aaaagagata ccgcgcgctt
    40501 ataacattgt tgttgctgcg accttacttt taaatttagc gctttcactt atatctttaa
    40561 ttcctggcac taatatttac tttactattg ttgatggcgt atatgctaga ggtaaattat
    40621 tctttgtcca aacaattatt ttccttttat tcttagcgct caccatttgt ttttacacgc
    40681 gtttctttaa aacatttggg aaaagtgagc gctttacttt cttacttatt ataactactc
    40741 tgatattagc acttgttggt cacttattaa ataattttat tccgtttatg gttatgaata
    40801 atatatttaa ctatatgatt ctggcaatga atgttcaaca tattcatgct tctactgatt
    40861 tcttgactgg tcttgctaat cgtcggagct taagtaacca ccttcatagt cgcatgactg
    40921 gtacggggaa aacgagcagt ttttccatta ttatgtttga ccttgataat tttaaaatga
    40981 ttaatgatac attgggtcac gatgccgggg acgacgctct tcgtcaattt gcgcggttct
    41041 tacgccatca ttgtgacaat gttgactttg tcgcacgctt tggcggtgac gaatttatta
    41101 tcgttcttga tagtatgtcg cccgctgaac ttaaaaaacg tgttgcggtc attcgcgaac
    41161 aatttagtga atatgaaggt aataaagtcg gtaattttaa actagtcttc agcgcgggat
    41221 actttatcca taacaaagat ttaaaaatta gcactggtga acttattcgc attatcgatg
    41281 agcggatgtt tagagataag cgtaaacgca gtaatggtaa aactaattaa aaaattttac
    41341 gcacacatag cgcgtacgta tattaataat taagtgtggc aatcacactt ttttatttac
    41401 cttcgttaaa tttcttttcc atgatgattt ctttactctt catcatccga attgtattgc
    41461 tgcgttctaa gtctgttagt ttcatgcgta tcacacgaat ttgttcttca atttctttaa
    41521 tgacaatatt ttcaatcgca ttaacacggc gttttgattt ctcaatttcc gccactaaga
    41581 ttttaatggt attttctaag ttagcaagtt cgatgagtaa cgcaaataaa tcttgaatcg
    41641 ctgtgattaa atcatcaaag gcactagttg tcgatagtaa atcataatta acttcagtgt
    41701 gactagcttt tgtttctaag ttaggaatga ctacacccat tacgcgatca aactttgcac
    41761 ttaattcatg tttcgttaca ctgtcattaa tatggtcatt aattgcttct ttactcattt
    41821 tgacacacgc aaattgatag cgaattaaag ttgcacttaa tgcactttca accttttccc
    41881 gcagagcgcg gtactcaggt agcagcggaa taaactcatg aattaattca tcttgtttgt
    41941 cttttaaaag cttgtgacca cgcgttgtgg tcgctagttt atcccgtaac cgttgtaact
    42001 ccatccgggt tggattaatt tgcgcagtgc tcattaatta ctctttactt tccttataat
    42061 atttatcaac gagttttgga tcaacacgcc gtaattcttg ccgtggtaac attcctaaaa
    42121 gtttccatcc aagatcaagt gtttctttaa tactacggtt agtgtcaaag ccttgtgaaa
    42181 cgtattctct ttcaaaagca tcggcaaaag ccgcgtattt ttggtcaatt tcagaaagtg
    42241 cactatcacc taaaataagt gataattctt ttgcatcttt accgcgcgca tacgcactaa
    42301 agagctgatt cattgtatca gcgtgatctt cacgcgtttt acctttacca atccctttat
    42361 ctttaagccg tgaaagtgaa ggtaaaacat caatcggtgg cgttaagcct ttcattgaaa
    42421 ggggacggga caaaataatt tgtccttcgg taatataacc cgttaagtct ggaattgggt
    42481 gcgttttatc atcttcaggc atcgttaaaa tcggaatttg ggtaatggaa cctttaagtc
    42541 cctttacgcg acccgcgcgt tcatagagcg aggctaagtc tgtgtacata tagccaggat
    42601 accctcttct accaggcact tctttacgcg cagcactgat ttcgcgcagt gcttcagcgt
    42661 agttagtaat atccgttaaa ataactaaga catgcatccc aagttcataa gcaagatatt
    42721 ctgccgcggt aatcgccata cgtggtgttg caatccgctc aatcgctggg tcatttgcta
    42781 aattaaagaa cattacagtc cggtcaagcg catcactttc ttcaaatgct tcactaaaga
    42841 agtttgcttc ttcaaaagta atgccaattg cggcaaatac aaccgcaaat ttttcgtcgc
    42901 gtcctaacac tgtcgcttgc ttagcaattt gcgcagcgag cctgttatgt gttaaacctg
    42961 aactagagaa aattggtaac ttttgtccgc gaacaagagt atttaaccca tctatggcac
    43021 tgatgcccgt ttgaataaat tcgtttggat aatcgcgcgc tactgggtta agcggtacac
    43081 cattaatatc aatgcgttta aaaggaataa tgtgcccttt gccatcaatt ggccgaccaa
    43141 gaccatcaaa ctttcttcct aaaatgtgtg gtgatacccc aaaagtaaca ccttcaccag
    43201 taaagatagc tttcgcgtca tcaattttta atccttgact actttcaaaa agttggacaa
    43261 cggctttatc attatcaatt tctaaaactt taccaagccg ctcttcaccg ttactttgaa
    43321 taatacgcac aagttcatca aatttaacac cgctgacatt ttcgacaacc atcagggggc
    43381 caacaacttc tttaattgtt ttaaatgctt taattgccat atatttattc ctccttcaag
    43441 ttgttaaatg ccgcaataac atctttttta agcgctttaa cttgtttatc aaaatctttt
    43501 tcttcgacat attttatatg acttatgcgt tcattaaccg caaggttttc catttgcttg
    43561 tattcaacgc cttcgtcaag tgcaaggttt gcttctttaa accaagtaac aattaaatca
    43621 agcatcccgt attgcttttc aatggaagta taagtatcta ccgcgtggaa agcgttttgg
    43681 tgtaaatagt cttctctaat catctgcgca gcgtggagtt tgactcgatc tttttgacta
    43741 agtgaatcaa tacccacaag acgaacaatt tctagaagcg ctgcttcgtc ttgtaataat
    43801 tcttttaaaa gagcgacata atctttccaa tacgggaaag aggcactatg atgcttaaca
    43861 atatcgtcat catacaaaga ataactccgt aaccagtcga tggccgggaa atgccgctta
    43921 tacgctagac tagcgcttaa cccccagaac actttaacaa tccgtaaagt tgcttgggaa
    43981 accggttcac tagtgtcacc gcctggcggc gaaactgcgc caattgcggt aatcgaacct
    44041 aaacgtttct ttttacctaa agtcacaaca acaccagctc tttcatagaa gtcacttaag
    44101 cgacttgcta agtaagcggg atagccttct tcgcctggca tttcttcaag gcgtgaactc
    44161 atttctcgca gtgcttccgc ccacctagaa gtagagtcgg ccataatcgc cacacggtaa
    44221 cccatatcac gaaaatattc cgcaattgta ataccggtat acatacttgc ttcacgtgcc
    44281 gcaactggca tatttgaagt attagcgatt aataccgtcc tgttcatcag tgataatcct
    44341 gttttagggt caacaagctt cgggaattcc attaagacat cagtcatttc attccctctt
    44401 tcaccacagc caatatacac aattaaatct gcatccgccc atttagcgag ttgatgttga
    44461 acaacggttt taccactacc aaatggccca ggaattgctg caatcccccc actagcgatt
    44521 ggaaacaagg tatcaatcac tctttgacca gtaaccatcg cttctttagg tggttttttt
    44581 gtaagataag gccgcttttt acgcactggc catttttgta acatcgtaag ttctttcttg
    44641 ccgtcaattg tttctaactc agcaattact tcttcaagag taaattcacc ttcattaatt
    44701 ttagtaactt tcccttttac tccaggcggc actaaaatta aatgtttaac tgcaggtgtt
    44761 tcattaacat aacctaattc atcaccttct tcgacttctt cgccgacttt tacagtcttg
    44821 ataaagttcc atttacgttt acgatcaagt tttggtactt gcacaccaac aggaatataa
    44881 gtgccatgta attcatagat tgaagtaaga ggacgttgaa taccgtcgta aattgcttcc
    44941 attaaccctg gaccaagttc aacggaaagt ggttcttcag ttaaggtaac aggttcacca
    45001 ggaccaaccc cagcggtttc ttcataaact tgaatacttg ccaaatcatc gcgtaattca
    45061 atgatttcgc caataatttg tttatcacca acaagcacaa cgtcataaac ttgcgctgtt
    45121 ttcatcccgc ttgctacgac aagcggacca gatactttaa caattttacc agttactttt
    45181 gccatattca tttcctctta tagaagatca atcccaattg attttctaat actttctttc
    45241 atctcactta ttgctttacc ttcatcttcc gtattaaatg ggagcggtaa ataaagggga
    45301 tataattgtt tttgattttt agtgtactca gtaaagaacg atgtaaaaaa ggtgtcatag
    45361 gctattactt ttacttcttt tggtaattct tttaagattt cagtaatttc ttcttcgtct
    45421 ttgacaacat ggactaaaaa gccaattgct ttaaaaagaa ttgcttcgct tttattacta
    45481 aataacacta gttcgtccat cttttatccg ttccttttcg tgtacacaat attgcgtaac
    45541 ctttcaattt gtaattgctt acgtttaatg taataaatta atgttggttt aaagtcacta
    45601 tccatcgcta gtatttttaa cttattaaat aagtattcat ctaacttttc ttctaactcc
    45661 actgttgatt cgctttcact agcgtcaata ttgaagttgt tttttaaata cgcatagata
    45721 gtgtgagtat caccatttac taaagcgcta aaagtttctt cacttaacac accctgtttt
    45781 aagtaattcg ctaataaact cgcggtggtg tcgcctcttt ttttaccacg cactagtgtt
    45841 aatatgttta agagcgcgtg cgtataatct aaataattta ctaaggcttc ttcttttctt
    45901 tgtagcgctt tttcatatgt ttcattatag aaatcacgaa gtgcaccttg gtctttttta
    45961 ataacatcat gggcaaagaa ataattaacg tataaggggt cgtttttcgt ataaagacta
    46021 acttcttctt ttaaatcact aagcgcgagc ttacatagcg cttcaaagtt cgtaacgata
    46081 cttgtcgaaa caagttcatt attaataaag aagttaagtc tatccatacc gctaaggttt
    46141 gcaaaagttg caaaatcact ctctgttaag agatttgata ttttagcgtc aagtactccc
    46201 tttaaataaa cgttattcat ctttaaataa ttcgttataa atcctttgct cgtgtttctt
    46261 ttgatagtta atgacgattt ctttgaagtc aaagattaag tcatactgcg gtgtacttag
    46321 caaaaagcct tccgcaacat gtgtcggttc tttacttaat gtaaagttat atttggcatc
    46381 tttcttattt aataagtcaa ggtttgtcgc atctttctta cttgaaagcg cggtcacaaa
    46441 tttcttaaag ttgcgcgcag aaacatgtat tacttcatca cctttaactt tttctttagc
    46501 aattaaatta gtgacaaatt ttaataattc ttcaccttct aaattcgcta atttattaac
    46561 aacctcgtca aagatgctta cgacaatttc ttgacgaact tgttctttat agttgtccag
    46621 tcctttttcg ttacgctctt tatattggcg aacgcgttcg acactttttg ctttagcttc
    46681 cgttaattcg gactcatttt gcgcggaaat agcgtccttt cccgctttta aaatacggct
    46741 ggcctcgctt ttagtttcct taactaaaag tgcagcctcc tttttagctt cttcagtaat
    46801 tcgttcaaag attggctttt tcataagcgc tccttaaacc ggtaccgcaa tgtacattaa
    46861 gacactaaca agtaacgcta aaattgcgta agtttcaacc atcgctgtca ttgtcatacc
    46921 acgcgcagac attgctgggt ttttaccagt catgctaatt gaagcagcag ccatcttacc
    46981 ttgataaatg cctgaagcta gcccaccaat cgcaatcggt aaagcagcgg caaataatgc
    47041 taaaccttgt tcattagtaa gagcaatcgg gtttccacca atcatcccaa ttttaaccat
    47101 aattaagacg gcgacgagga acccgtaaat cccttgtgta gctggtaagg cttgtaaaat
    47161 taacacccga ccaaataatt ctggtttttc tgagattaca ccagcggcgg ctttaccacc
    47221 taaagaaaca cctactgctg aaccaacgcc cgcaagacct gcggcaagag ctgctccaat
    47281 gagcgcatac actaatcctg ttgtcattta ttcattctcc tttattgcat taacatattt
    47341 taatttgtac gaaaacggcg taaattcata accgccacct tcgtagaact tcccaaaaaa
    47401 ttctaagtat tgtaaccgtc catcatggac ataggcactt agaaggccca ttgcaaagtt
    47461 aaagacatgg ccaattaaat acacaatcgc cgcaaataaa atgccgacga ctggaatacc
    47521 gccaaccatg cccgctaaaa gattcatcgt aaaagcaata actgctgtac ttaaactaag
    47581 cgctaataac cgtgaataac ttaaaacatc acttaagtaa cttgtaacat tgtataatcc
    47641 gcctagacca gcaagcgctt taccaatgat ccctttctta tcgcgtccgc taaataataa
    47701 gataaaaata gcgccaatcc caatcatgcc gtaagctaca tagagcacaa tttgattttt
    47761 tacaacaacc gctatcacta atcccactaa gattaaaatc caactaacag cgtcagcaag
    47821 cattgattta atatcacgct ttctcgcatc attaataatc tttagtacta aacccgtaat
    47881 aatatgagca attcctaacg ctagtgaaaa gattaacatc ggcagcggat cttcaacggg
    47941 ttgcagtact actgaagtta agtttggatt tccaaaccat ttaccaacaa tatttaataa
    48001 atcgacagaa acaccaaaga aactgccagt aagtatcccg aaaataatcg agggaatact
    48061 tgaataaaga aagatagttg ctaagtcttt aagtccgcct ttaggttttg ctagttttaa
    48121 aaataaccca aaaataatta gcagcgctag accataaccc atatcaccca tcataatccc
    48181 gaaaataatc caataccaca ctgacattgc ggggttagga tcaagttcat tagggttagg
    48241 aacactaaac atgtttgtga tcgattcgaa tggtttcact agcttattat tttttgtcgc
    48301 agttggcacc atttcttcgt tttgcactgc taactcttca atatcacaat caactttatt
    48361 ttctaagttt tttgttaagg tttcaaactc atcttcacgc acccaacctt caacataagc
    48421 gacgcgttca gtcgttttag ttaccacgag tttacgcagt gctaaattgt aataatagtc
    48481 gtaataaatc cgtaactttt cttcatcgtt acttaacgct tttaaactat cttttgcttc
    48541 taaaatagct ttgtccttat tcgctaaatt gtcatttaat tccggcaaaa gtgctttaag
    48601 ttttgtatca tatagtggta gtgttacttc agtaaaacga tatgaattaa gtaaaatgag
    48661 cgcttggctt tcatacacta cttcataacc aaaagcgcca tagacaaatt catggtcgcg
    48721 tgatgactct tcaaaaatat gacctttttc gtccgcttct ttagcaaaat cttcaaataa
    48781 attaagcggg acttgtccaa taacaatccg tgttcgcact agttcactaa gtggtttagt
    48841 ggcaacactt aaatttaagt acggcgttaa tgttttaatt tctgcttgta attccgcaat
    48901 ttctttttca gcgttttcaa ttgtttttaa agcgctatta attttagaaa gcaaagcttt
    48961 accttccgct tcaatcgtgt taaattcact ttgtttaact tcatgatagg caaaaaactt
    49021 tttctttttg tatttgctaa gcgctttaat cccgcgctca atcgctcgaa gttcattatt
    49081 aatatcacta ggaaggcttg gatcgttttc atgttcaaaa agcataaact tgctttcgtg
    49141 ttgtaatgct tcaataagct taccttgttc actgattgcg gttaatagtc taattttctt
    49201 tactggaaca atcatttttc tacaatgtcc ttaaagactt tatccactag tttactttgg
    49261 tttgcttttg cacttttttc aatctcatta gtgattgttt cttttttcgc agcaaaagtt
    49321 gtcgctagtt tttcaatctc gtgatcagct tttttattaa gcgcgttaat ttcacgcgca
    49381 attgctaaag cattttcagc gcgaagtgtt gctaattcat cgtgagtatc acttaataat
    49441 ttcgtgttat cttctcttgt cttagcgagt aattctttcg cgtcaagttc agcttgtaca
    49501 attttctcta atacactcat aggactcctt tctaggcatt ttgtccgcta ccaatttatt
    49561 tatataaata ggtagagttt tgtatccacg tgtagtattt taacatgttt acattgctaa
    49621 tgtaatgtga tacgactatg tgcttccgct aaaatgttga atgtatacta aaaaaccgca
    49681 ctatgcggtt attttagata agaacataat agttaaaact attttttgcg tttagcgttt
    49741 tttatatatt caattgccgc gtcaacttca agtgggcggc tcatataata cccttgcccg
    49801 aattcacagc ccaagccttt tagggcatca tattgttctt tagtttcaat gccttctgcg
    49861 agtgactgaa tattaaactt tttacaaatg ttaataatat cttgggcaat accttgctca
    49921 gcgttttcgg catttattgg tgtcacaaaa acgcggtcca ttttaaccgt gttgaaattc
    49981 aacctttcga ttctcgctaa tgaagaaaag ccagtaccaa agtcatcaat tgccgtatca
    50041 atcccgtaat ctttaatttc agcaagtgct tcacttaatt gttcatagtt tgtagagaaa
    50101 attgattcag tcatttcaat cataatcatt tcaggcggga cacccgctga ttcaatgcgt
    50161 gcaaaaaggt cttgggcaaa atttggatct aagagttgta atacggaaat attaattgcg
    50221 ataccacaat catgaatccc tgttgcgagc aattcttttg caaaggaaac cgctaagtta
    50281 ataatttttt taccaaaagc aataataaga cgattatttt cgacaattgg aataaattct
    50341 gacggggcaa taggcccata tttatcacta gtgaatcgcg caagtgcttc aaagccacgt
    50401 actttaccag tttgcaaatt aataatcggt tggaacatga gtcgtaattt atgttcattg
    50461 cctggctcgt aagatatagc aattaactct tgacggaggt gttcttctcg cttcactttt
    50521 tgatccatta tatcgttgta gatatggata gaaataagcg cattttcatc atcaataaat
    50581 tcacttgcaa tccgtgctct ttgtaagtac acttccgcaa tgctaatgct cttatgtaag
    50641 aatgttacac cgataccaga gcgaatttgt tcgcgcgaaa tagtgtgacc aatcgcactt
    50701 ttaagttcag cgtaaaactt tttaatttct tcgcgtgtca tctgatattt agcgtaaaat
    50761 acaaacaaat cttcttcagc ataatgcagt gttattctgc ttgtttcaat tgtttttaat
    50821 ttagtaacaa gcgaagctgt taataattcc atatattgat agccatgtga acgatataat
    50881 ctttgacttt cttttaggtt cactaaaata atcgttccgc tataattttt actatctaat
    50941 tttgcttgta ttttatcaat taaatattcg cggtttggta aattaagttt ttgatcatac
    51001 tgttgaaaat gtttgagttg tttttcaatt ttcttcatat gattaatgtc ggtaattatc
    51061 ccttcataga ataataaatt acctttttta tcaaatactg gttttgcagt ttcacttacc
    51121 cattttttag tgccattcct cgtcactatt tcatattctt ggcggaattg ctttttactc
    51181 tttgttgctt catcaaatgc ttgacgaatt cttggtctat gaacacgcac aattaaatcg
    51241 taataggaaa cagtcttatt atcgattaat tcttcaggtc gataccccgt taaggcaagc
    51301 gctccatcag acacaaattc cattgtccaa tttttatcat taagtgagcg atacgccata
    51361 cctggtaaat tttctaataa agtagcgcga gcgcgatcac tttccgctag tgtgtcttca
    51421 accgcttttt gtttcgtgac atcccaagat attactatcc gtgaaaatcc ttcatatttt
    51481 tctgatttta ttatcgaaat aaattgttca acccatacga tgttaccgtc tttttttata
    51541 acgcgtttcg tgacattgaa actattttct ccaagtagtt tagcttgttt ttcaatttcc
    51601 gctactcttt gcaaatcatc aggatgagtt tccttattta atcccaactt tttatattct
    51661 tcgcctgtat aaccaagaac ctcatcattt tttcgattga aaagcgctag ttctaaatta
    51721 tcgttgggga taatcatgat cccaaacggt gcacgttcaa aagcagcgta caccagtgat
    51781 attttttcaa ttgtggaaag cggatattct tttaattcat atatcttatt taataagtcc
    51841 gcagcggtgc ttgtttccat acaccaaaag ccaaaacgtg cctcttcatc aattgactca
    51901 taaaattctt tactcgctaa cactaatagt ttaaggcgag gatactttaa atatagacga
    51961 ctaaactgct caacgctttg aatgttttcg tctttcaatt caaaaataac gccacagagt
    52021 tcagggtgtt tttcaacctc attaaacatc tctgatggcg tactaaaaaa taatatgaaa
    52081 tcattctcaa gcgcagtttt gactgcgttc ttggtattaa aattattact gacaactaaa
    52141 atttgtccgt ttttttccat ttgatcccgt ttctattata cattataaca tctgttatac
    52201 atattttttt gttttttgaa aaaaataaat attattgagt tttttcggtg atttttcgct
    52261 ccttaaaatt tttaaaaatt taaaaaaatt gcaatataaa aattaataaa atatataaat
    52321 gttttgcatt ttctttagca acatatactc aaaaatcata ataatattaa aagttttatc
    52381 ttgcaaaaca atgttatttc ccttgcattt cgtggttatt ttgcactaaa tacaattgct
    52441 tcatctaata ttttttcgtc aaagttaaag tttttatatc cttcgcgtac tcttcttata
    52501 tattcctcac taggagcgcc aagaatcctg tcttcatgca tgatatagac aaaggcatta
    52561 attgttgttt tctttccgtt ttcacttatt aattcaacaa caaaatcttt tttatagtaa
    52621 agtgtcggaa atccttcata acggtcaagc gctacttcgt caccttcttg aataataaaa
    52681 acaccaactg gcacttgata accctttgct ttttcaatcg ttaggtaatt gctattttca
    52741 cttcccttaa acatcaagcg atagtcttta attattgctt taccaacttt tttcgatgtt
    52801 ccacaccgct cctgcatttg tgcaagatta agattgctgc cgtaggctaa atagtaccgc
    52861 aaatctttgt gactaaaatc attaaaattc ataatatttt ccccattacc ttgtatttat
    52921 tgttcttttt ttagcactgc tttaaattcc ttcgtgcgtg tccaatattt aatgccccaa
    52981 ttttcaaaat tccattcttc gctgaactca ttacattcgt aatcaacgta atatattaag
    53041 ttgtcttgca caacaaaatt cgtggggtag taatcaatat ttaggcccgc ttttttggct
    53101 aaagctgcca tttctcgaac ttgatcaata aataaatcga tatttacatc tctttttaca
    53161 tattcaaaaa ttgtgtcgcc tttaacatat tctttaatga taatctcttg gcctttacaa
    53221 atatcaatta agcgcggcat tctaatgccc gccttgaata atcgttgata atcgtaatat
    53281 tctgcctcaa tcttgttgcc aaaagtataa tattcacatg gttcatgatg aatcttttta
    53341 accacgactt ctttattatt aaaagtcgct aaataggaat aaccaccttt ccctttaccg
    53401 agcaatttta ttagtgtgta agttttaccg tttaccatta cttctttttc catagttcac
    53461 catcagtata gcgtttttaa cccttaaact aaagaccact atcgcttttg aaggtgaaag
    53521 ctggtaaaat aaataatatg aagattatat cgaaaagtaa ttacacacta tttaaaaatt
    53581 gtccgaagtc actgtggtta tctttttata aaagtgatgt cgcagcgcct ttaaccatgg
    53641 acgttttaaa acgaattgaa gacggaaaag aggtcggaga ccttgctaaa aagtattttg
    53701 caaacacaat tgatgttaag tctttattgc cagatggttc gctagacatt tctaaaatgc
    53761 ttgaactaac taaaaaatat ttaaaacaaa aaggtgtcac aatcgctgaa gcttctttta
    53821 aagtaaataa cctcttctgt tctatagact tattacgcgt cactagtgaa ggctatgaaa
    53881 tttatgaagt taaatcaacg accgaagcaa aagtagaaca taaggttgac gctgcatttc
    53941 aaaagtatgt attacaacaa tatggactaa atgttattaa cacatatatt ctgcatttaa
    54001 ataaagacta tattcgtaaa ggtgatttag aattagatca actatttacc atgaatcatc
    54061 tagatgaaga cacatctttc atagaagcaa tgcacaatat gaaaagtgat ttaaaagcta
    54121 ttgataaatt actgttaaca aaagctgaac ccgctgaaac attattaaca agatgtaaaa
    54181 attgtgactt taaagattat tgtcaaaagg atattcctgt tccaagcgta ctagatgttt
    54241 accgcctatc aggttacaaa tatttaaata aaggcatcgt cacttttgaa gatttattag
    54301 aaagcaatgt tgctttgcga aaacgacaaa gtattcaagt tcatgcctat ttagataata
    54361 aagaagtcat tatcgataaa aaagggcttg cctcttttct aaagaaaatt aagtatcctg
    54421 tctatcattt agattttgaa tcctaccaaa tgccaatccc accatgtaac cttatgaagc
    54481 catacgacca aattcccaca caatattcat tacatattga atatgaggac ggaagtttag
    54541 aacataaagc ctttcttggt aattcacttg atccgcgtcg cgaaattgct gagtcattat
    54601 gcgctaatat tccgcgtaat gcatgcgtta ctgcctatta taaaggcttt gagtgtggtc
    54661 gcttaaatga actcgccaca ttatttcctg atttagaaga gcatttactt aacataagta
    54721 agaatgtcat agatctatta gaaccattcg aaagcggata ttattaccat aaaaacatgg
    54781 gtaaaagtaa ctccatcaag gcggttttac cagcgctata tcctgatgat tctgaacttg
    54841 attatagtgc gctgcccgtt gtccataacg ggagcgaggc tatggttatg tatccaataa
    54901 tgctaggagc aaagaaggaa gaaaaagaaa gaatccgtaa cggactctta gaatattgtc
    54961 gcttagatac tttagcaatg gttaaactct tgcaaaaatt acgaaaaaca caataagtgt
    55021 tatttatata gttcaagtat ttcattaagg tagggcactg attgggttgc cccttttctt
    55081 gatactgcaa tacttgaagc ggtcgcggca aagcgtaatg aatcttctat ttcgtaacca
    55141 tttgctttcc gatacgctaa tgcacctaaa aaagtatcac ccgcacatgt cgtgtccaca
    55201 acatttgtgg gtattgccag ctgataaatt atttcgttat ctctaattaa atacgcacca
    55261 tcttcgccaa atgtaacaat cgttgacttt gcttttaatt tatcgtgtat ttcctgcaaa
    55321 tcgttgttat ttttataatt aacccctgtt aataacttgg cttctgccgc atttaaaatt
    55381 aataaatcta ctttcgacaa tatgctttta ttaaattcac agactggtga aggattaata
    55441 ataatataca ttcctaattc atgtgcaaaa tctattcctt tatatagcgc ttctaaattt
    55501 gtttctaatt gtgcgatgaa tatatcgcct ttacgcgcat atttctttaa taagtcaatt
    55561 gctttttggg gggcattcat caaattagcg ccattcccga cgatgacagc attatcttcc
    55621 gcgctacgat taaaaataat aaaacatgtc ccgctaggct cgcctttaat tacttcaatc
    55681 ccgctagtgt ctacaccatg ttctttaatg cttttaagca tgatatgacc actatcgtca
    55741 tcaccaaccg cactaattaa aaatacttta tcaacaccaa tctttttcgc cgcaatcgcg
    55801 tgattagcgc ccttgccccc taaattagca atcattcctt gaccaattat tgattcacca
    55861 agaatcggca ttttatcggt atacactact acatcatagt taattgatcc taacacgaat
    55921 actttcttca ttacttaatt gcctccgtta ttttttacac actacattcg gaaatattta
    55981 gtgttagatg atttgccaat tttcctgact ttaccactct taaccatttt tcctaaaaca
    56041 tattcgacgg tggtcacact tatgtctggt aatatttcac atatctcacg ttttgaaatc
    56101 ggtacaattg aatttaaaac cgttgcttca atccgcgcgg tcttattgac tttttcactg
    56161 tttacggtta gaaacctttt atctaattct tgataacaat aaaagagcgt tactaaaaaa
    56221 ttctcaataa aaaagaaata gttattatta ttttcatgcc agccaaccga ggatttccta
    56281 agtgcttcat aataaaatcc ttttctctta ttaatttgct cttcaaaaga aatatacttg
    56341 gcagcgtcaa aaccagcttt ataaagcaat aataatgtta agagtctaga aacccgtcca
    56401 ttaccatcac taaatggatg aatacacaag aaatctaata tcacgcacgg gatcaacaat
    56461 aatttattaa tcccactttc agcaacggca atagtgtaag caagtattag ttgttcaatt
    56521 gcgggtgcgg tttcacttgt cggagtcggt ctaaacctaa catttcttgt tccatcaggt
    56581 ttaatttcta ttattacatt atcactagtt ttataacgac ctgcattttc aggacttgaa
    56641 accgctaaaa gcaatcgatg taaatctaat attgtctttt cttcaaattg taaattttca
    56701 tatgtattat gaataatatt taatgcatcg cgatatccag caatttcttc ttcgtcatga
    56761 tttaagggcg cgctattttg attaacaatt gctttaattc tttcgtcaga agtaacaatc
    56821 ccttcaatcg cattactacc tttaaccgac tgaactttag caatttttat aagctttgta
    56881 taaattgatg catatttatg ctttcttgtt tcatcaagtg ttcgtaaaga ataaatactc
    56941 gaaattaatc cgattaagct cgctggaacg gacccgtctt ttaaaaacaa ataatcaaat
    57001 tttctcatac tgcataacaa cctttattct ctgcatatat tttactaatt attatgcaga
    57061 gatgcaatta ttttatgtgg tcgtctgcat aattttgata aatttttatg cagaaatttt
    57121 cttttacata atttaagtat taattttaaa taatgaaaga ccagatttaa aatcaaagag
    57181 gctcatctta ttttcatcaa atgttagtgt atgttcttct cccattacat atttatgctt
    57241 gccaacattc tcttttgatt taaatttcat accattaaat ttaaagtata aataatattc
    57301 ataacccaac aattcaacaa aatcgattgt tgcttttatt tctggtgctt cattttcgaa
    57361 ggtttcacca gcaatctttg gtttaatatc ttctggacgg ataccgaatt ttatcggtag
    57421 tctttttttc gttttacatt ccttataata atcgatattt ttcatataat cagttatttc
    57481 ttgattataa acttcaattt catcgtcttt ttctattctt tgccttgcat gtttatcttc
    57541 tgttcctgct tcttttgcaa gcaaagtttg ataattacta tttattgttt ttaattcagt
    57601 gtctttgcgg gtaagaagca catctaattt tcttttcaag taatcaattc cttcttgaat
    57661 accatcggtg tcaccatccg ctaaagcttt ttctaggtta gaaatgtttg tgcggtgcaa
    57721 accttctttt cgtgcatact ttctttcaag tttaaacaag gagttaactt tatagcgttc
    57781 ggcttttgaa gacagataaa gttttcgctt gtctaatttt ttggatgttt ctagcaacaa
    57841 ctcttcatat tcttttatct gtttattgat aaatgacatg actaaattat ttttatcttt
    57901 atctaatgag aatttgtaac catcttcaaa tttaataacc ccattatcat aaatagcgtt
    57961 aacagcattc atcgcaggcg aaccaataaa tgttgctaca aataagttgt tcggatgtga
    58021 ataaacttct tctggtgtgc caatttgttg aacacgccct ttatccatta cgacaatacg
    58081 cgttgccatt gtcattgctt cagtttggtc atgagttaca taaactgtcg ttgctccaat
    58141 ccgattatga atcgaaacaa tctcactgcg catttgaatc cgtaatttag cgtctaagtt
    58201 tgatagtggt tcatccatta aaaatacagg ggaagcttta acaatcggtc ttccaagcgc
    58261 tactctttgt cgttgtccac cacttagtgc tgacggcttt cgatctagat attcgtcaat
    58321 ttctaatatt cttgcagctt tttccactct ttcagcaatc acatcttttt ttagattttg
    58381 aatttctaag ccaaatgata ggttttggcg gacagtcata tttgggtaca atgcataact
    58441 ttgaaaaacc attgatatgt ttctattttt aggttcaatg tcattagcgt atttgccatc
    58501 aatatataaa tcacccgcag ttatttcctc taagcccgcg atcattctta gtgttgtgga
    58561 cttaccacag ccagaaggac cgacaaagac aataaattct tgcggcttta tatctaacga
    58621 aaaatcaaaa acagcttgaa caaaattagg atatatttta tttacatttt gtaaggaaat
    58681 gatggcatct ttcggtgcaa tatttagttt ctttgattct tcaaaaccgc gcgccgcaac
    58741 gttataataa tttagctctc gttttttatg ttcaattatt ttgtttcttt tttcatcttt
    58801 tgactttttc ataatatgtt ccccactagt gcttcatcta tttctttagt aatttcttca
    58861 ttacttttaa ttggtcttaa ataataatgg tggccatctt gatctttagt aaatgtcgtg
    58921 aatccgttat catcaatatg cattacccca ggcccaaccg atctatataa gcgtttagta
    58981 attgcataat aaaccgctaa taaatcccaa cttcgcgatg gcccgttagc gtattttaaa
    59041 tatgcaaatt tcattggtga atgttcatca tgttcaatta ctttctttcc tgtgaaaaca
    59101 tcgacgccaa cataaaaatc taaaaaatag atgggttttg ccgagatttt aacaaacttt
    59161 tgtgcatctt caatcgcaca aacaatatta tattcaggga acgcttcatt attattaaaa
    59221 gcagtaatag gatgattatc atttattaat ccacccatta cacaactata ctttactttt
    59281 ttagcaaata attcttggcc agataatggt gatatttcat caggtaaaga acttaccaaa
    59341 ttcgctgcat tagtgagttc accaatcgtg ataaaaataa ttgaatcatc ttctgcttca
    59401 gcaagaactt tacgataaag tttatatgct tcaatataat tagtttcttt tattaaatct
    59461 ttataatgat cgactaaatc aatatcaaaa atatgttggt cttcgttgca ttcaactttt
    59521 ttgccataat acattccaat tggaaatttt ccgccataga aagaacgcac aatatcaaca
    59581 cattgcaccc cataaatatt cgtcgtacaa taaccgactg ccttaatatc gcaaaaccca
    59641 tgcttttcta attgcaacgc taacacaagg gtcccaacat catcacaatc agcgcctaaa
    59701 tcagtatcaa taatgacagc aactttattt ttgaccattt agtaacacct ctttgtttgt
    59761 cccgtaaaaa acatcttttt tattagcaat atacgctagg aatttttcaa attcttccca
    59821 cgcatcttca aaatccaatt cgtaagaatg cccccaaata taaaatatct tatcctcata
    59881 atattcagtg ttaagaaaag tgcctgctag ttcatacatc tttttaaaat ctaaaaaatg
    59941 aattgttggc ctaaacgcta ataagttatc ttgtaaatca aaattgtatg tcgcccaaat
    60001 acttcttgcg tatttaatcg gcgtatttct tttaataata ttaactatcc tatcatttaa
    60061 tgtgccaaaa ggatacgcca tcccaactat tttgtcgcct gttaattttt ccaacacctt
    60121 tcggtcttgt tcaatctcat caatgatgat tttttcgtca gcgattgttg ttaaatcagg
    60181 atggttaaca gtgtgaaccg ctacttcgtg gccgctatat aaagccttaa cctctgcgct
    60241 atttaccttg ttatgtggtg cactccattt ttgccagtga cagacaccat ctttcccaaa
    60301 accaccacta tttagattga aagtgcattt taaattatac ttattaaata atgcaacgag
    60361 gcggcgatct tgtgttaccc catcatcaaa ggaaaaagtt atcgctttat gtgtattatt
    60421 tggaaacatc tcaaaccttc tccactaaaa tatcatcaac aatcattgtc ccaatgccgt
    60481 ataagccaat aataatcatt gtgtcatcgc aacttggcgt cattagctca aaggtctcgg
    60541 tataaactgt ttctaaagta ctggcgctgg caaaatgaat ttctgttccc acaagtaacg
    60601 gattatcagg attaccgtcg acatgagtag ccttgcttcg tgcaaagata taggcatagc
    60661 cctttagtga aggatcacta tgtagcctaa tctccgtcat caatttatat tttaaagtta
    60721 aacgataagt tgaatttccg ctaagtttta aatacttgtc atttgagtac atgaattcat
    60781 agtaattatt cttatcttgg aattgatatg taacctcgtc taattcactt gtaaatgaca
    60841 atttttcatt aattacctca ttaggcttat ttgtaaagtg accccatcta ttaaaaccaa
    60901 atgagaataa gctatctgct aaaacaccat cttcaaaccc ttcttcatag ccaagacctt
    60961 tatttggtgt aaatggtcga acattattat caaagcccac atcttcgcca tcaacaatat
    61021 gaccatttgg taattcgata aggctaatgt tatcaacgta atatccgccc tcatttttaa
    61081 taccaaagac aattgttaaa tccttactgt tttctggcgt ggtaaatgta aatattttgt
    61141 tattcgcggg cgcatcgggc ctatcatacc attccccaat tgtcatgata ttttcttgtc
    61201 cagagcgtaa tttaaggaaa taataagcac cgttgccacc aacgcgatat cctttagtgt
    61261 caaagcttat tttataagcg gtgttcgctt ttaatttgat ttttccttta ttactcataa
    61321 gcccttgttg ctcaccatgc aaaacattac tttcgattaa gacaacattt gaaccattaa
    61381 tgtctttttc ttcatcgcga taaatatcag tataaatact gtctgtatac cacgagtcag
    61441 tttcaatatt atatttccct tcaaaattaa tgagactgct gctgaccttg tttatcttaa
    61501 tatttttata acttatttta ttttgataaa tacctgatcc cgctatttct aagtggtatt
    61561 tgccgtctta tttaataaat ctttcgcaaa cgccttggcg gttaataaat ctaaatcgta
    61621 aaccgtaaat tcagcaatct tgaaatcaga tactgtaccc gcgatgcttt ttgaaacaaa
    61681 tcgaatttgt tgttcaggta atgtcgtaga acagtagaaa tcaatcacta atgtgttatc
    61741 actccacgta atatcacaag tttctagtcc gctatcatgg gtaacttcac ccgttactgg
    61801 attaatcgta aatgttgtat gtccggccca gttcccaacc atattaatat acatttcggt
    61861 tgtggttgga gcttctaaaa atttaataac attccgatag tttttacctg ccgtatatgt
    61921 tagtggtaag tgattaaaga acattgcttc ccatgtatca acagtgttgt ttgtaccata
    61981 gaaatgtaaa tatttttgtc cacttgctgc agtagcaggt tctaaagtgc catccgcaac
    62041 atagtaatga tcttgttgtg atgaacctgc aattgtccca tagttatctg ttgaatagac
    62101 atcatcaagg cacactactt tgaaattatc aattttgaaa gaatcgccgc ttgccatatt
    62161 agcaacaaat atctcaaaat agtgtccacc tgccgaacat gtaaaggttg ctgaaacttg
    62221 tccatcaaca tattgaacat ttcggactgt accatttgaa ctctcggtct tgtatgcagt
    62281 agttttgccg actttaaaac ttgtccataa tccttcaata ttaaagtcaa actgcactgt
    62341 cgcattatcc gcaacgcttg ttactaatgc atcaaattcg acacgatata atttaccgtt
    62401 ttcaaataaa tcattaccag taagacctgt ttgatgtgtg ttaataccac ctatttgtgt
    62461 agctccacca tcataagtgt ataaaagtga atgattgcca gcattttcga caatgctcat
    62521 cccgttgccc caaaatttgg tgtcttgata aatttcacca acaatatttt caaagtcttc
    62581 atgataaacg actgtttcgc gtgtcgcatc attttgttta gcttccacta ggcttttgtt
    62641 gggcgttaaa aatccgccaa gcgaaaggga cgctaaaatt attggaatta taagttttat
    62701 aaaattacct ccatacatct ccattctaac atacatgaaa agcgtaataa aaacattgtt
    62761 gttgcgtatt gatgtagttt tgttgttttg ttaataagtt attacttctt atttttattt
    62821 attctaaaac cgaccaatca tcaaggaaat acccgttgat ggtgaaatgc acaacatcac
    62881 tatgaataaa ttcattcgtg ttacgatcta ggtaagagtt ttcactatag ttaaagttca
    62941 tgttttgttg aataatcttt aaactattta aatcaactgg tgcagcatac ccgctaccgt
    63001 taacattcgc atgtacttta atatatagga aatacttttc atcttggctt ggatcataaa
    63061 ttcgtgaaat gttatagaca aaatcaaacc ctgttgccgc tgtgacaaga taatgatcga
    63121 catttggatt agtttcagcg tcgtaattag gattggttgt taatccaaaa tacggcattg
    63181 tcattgatgg tacgtaatct gttctaccga ctgtcgcaat catgtttgtc gtatttttaa
    63241 atacatagtc aatatttggt aaagacggga tgacaatatc gttttcgtgt aacgggttat
    63301 tatcattggc tgcgacgcgg tcacttgata agtaaacgaa acacgctgtt ccgcgcgatg
    63361 cttcaaggaa atatgtaatt ggtactgttt caattggtac ttcgaaagag taggcaatta
    63421 agagcctgtt aaagtttgga taaacagcat acgtcgggct tgatgcactc gtttttaagt
    63481 acactggttt agtgccaata attgttgaac ctggcggtgg cggtaataca aagccgctta
    63541 ataattgccg gtcaccaaca gttgtggttc cactaccaaa acggcttaca gtaataagtt
    63601 gttcaacgcc ttgacttggg tcagtagcga caataacatt aattgttgct gttgtttctg
    63661 ttgaatctgg cgttttagcg ttaactgtaa acccaatact accttctggc acattaatag
    63721 gcggcccgtc gaccggcgaa ggaatcccag tattaacacc gaagaaacgc tcaaggccag
    63781 aagcgttttt aatatagtaa taagaagtag gatcaccgat taatagtgca gtgtcccctt
    63841 ttgtaattgc tttattccgg ttatcaatcc acccgagaga actaatatct ttaatttcat
    63901 aactatagcg tgaggtcggt acacttgaat tataagtacc ggtgtaaaag agcacattat
    63961 gttcagtatc tatcgtctct gttgttcctg ctggaataaa ttcttctaaa atattacggg
    64021 ttgccactgg gctttcactt ggattattcg tgttattcgt gtaacgcaca gcgtaaagtg
    64081 caaaggaaga cgcgtaagtt cctgggcttg aagtactaac agcgctaaag ccttcggcag
    64141 ttgaataagt catataccag tttgaagtac ttccaccact actactagca ccagtccgtt
    64201 gaatataaaa cttattaaaa ttacccgtat ttgaagtagg actaatccga tacccttcaa
    64261 cataaggcgg gttgctcgta gacgctgttg ccatgaagct tgatgttggc actgcattac
    64321 tttgcgcatt aacccgtaat gggcgcggtg cttctaaaat tttagtttgt ccgtccacta
    64381 ttccggttac aatgtcgcca taaacggcgt attgtaaagt ccctgcactt gaagtagtgt
    64441 ttctaaagtc cattgtgatc ggaccactaa aacgataaga gaatccagct tcataaggcg
    64501 gataagtaat atcagcagtt ctttgactta agttttcata agtgaaatat ttaaattcac
    64561 cgtccggtat aactaggtta ttaggatcta cttccacacc aatcgcagta gaaccataac
    64621 gcacaagtgc ataatgcttt gtcggattaa cagtcgtgtc ttcatacaca agcatataac
    64681 ggtaatcagc attactacta tatggtgagt ttagtgttcc gtaatactgc cgattgaacc
    64741 atcccgtata attccaactc atttcgttat tagtaatgcg tgttgcggta tattttgtcg
    64801 caggaccgac gtcgcgcgtt gtttgtgtat acggatttgt tggattagtg gaaattgtcc
    64861 actcattagt gtaagcatct gtccaaatct tagcgatacg gtctttcccg cgtgactgac
    64921 gactgtgggc aaaagtaaat acaccaacgt ggaaatatga attatcattt ggatcataaa
    64981 gttcgtcacc aaaagtgtct tgtaaatcat aagaattatt cggagcagga ttgccaacag
    65041 tcgtattttc ttccatgaat ttccaaatat cttcagcgta aacaatcccc ttacctaaac
    65101 ccgctgcagc ttgaccagca ccttgatcac taccgtcact cgtcactggg ttttcaattt
    65161 caccgtcaac aataacgcgt ttataaaaac caataattcc gccggaagtt gtatatttag
    65221 cggtgtcacc agcgctgaca ttaaaggcgt caacatgggc aacgccattt gtttgtgaat
    65281 aataaacact gattttgtct aaatttgcgt attgaacgtg acccgcaagg attcctaagt
    65341 ggtttgtttc tttagtaacc cccgtcttag cgtaaccgta gtggccgctt ggcatgacac
    65401 caacggtgct agtgttaatt tgtatgttat ataaaaggat attagaaatt tccccttgat
    65461 agtcggtttc aggaacttcg ccttcaagat ctggaccaac acaaccaaat aacccaatat
    65521 caacttgatt ttcttctggt gtaacacgaa cattaccaat aatactagtg tcggttaatt
    65581 ccccagtcgg taatgtcaca tatttatttg gatcggttgt ggtaatccct ctaattttgg
    65641 atacgaaagg atagtcttcg ttgccaattg attgaatcgt aaataattgg ttcgcggttg
    65701 ctcccccaac ataaattggg tccccgtttt catcagaaac ttggaacacg ctgttttcat
    65761 taacataatg actataagca ccattttgta aaactgaaag gtttgataag tgatcggtgc
    65821 tcataattaa atatgggtta gtgtctgtac cccattcgcc agttacgaga tcttcacgta
    65881 aatgaccaaa aagtagttca atttctagcg ttggttctac ttgtctttca atattagcgc
    65941 ggaaataagc atatactgga tagccaattg ttaaaacaaa agcaaagaaa acggtaattg
    66001 aaatccatac tttactgaag aagtttttaa atttatgcgt tttcatctaa tgttttaacc
    66061 tccccgttta atgacattct aaacaccata ttatatggtg ataattggat tggaacaagc
    66121 cgaataatat tttgaacctt agcaatatct ggttttaaca aaatgtaaat ccatgtatct
    66181 aaagtcgcac tattgctaag taatgcgtgt ggcaaagttg tgattttaaa tggggtcatt
    66241 gtttgtaagt tcgcaaatac agtcaaggga ttaagattat cacttgttga agtatagaca
    66301 taaaagtcaa agacattttg tgcattttct gctgcgaaat aatcaacacc agtaactgca
    66361 tgaacaccat caatgttgga aataactgtt tcaatattgt ttactatcac attgtattta
    66421 gtttgtgcat cactagtttc attgattctt atttttaaat aagtattgct attagcagga
    66481 atctgtgcga tattcgtcat cttccctaaa taaatactaa gcggttctgc actcgtacta
    66541 ttttgccaac tactcgttgt gtaattgtag tgcgctagtg agatttcggg cgtaagttca
    66601 ccaggtacat tatgtgtttg tgcaaaatta taaacggaaa tatgccacgc atatgttaaa
    66661 gaagcgcttg ccacaagtga cagcaccatt aaccaaatcg ttctaacaat attgcgtttc
    66721 ttttctttca ttttatattc ctcgatagaa gaaagcactt agcgatcttc ttgatacacc
    66781 tgttgtcggc caatcaacaa aatacatact ttggtcagtg ccattatcca caaaagtgaa
    66841 ttctaaagta cctgttaagg ttttattcgc aggcacagaa acattttgga taatataaaa
    66901 gcgtgctatt gatgttaatt catttgcgac accattaact tgtcctttaa caacaccaac
    66961 actatttgtt tccactaagt gattgcctgc accacttgtg aaagttggtg aagcaccgtc
    67021 aattgccagt gaaacagaag ttaaagcaat ttgactacct aaataatagt aataatttgc
    67081 attaccccat ttacccgttg catcaatata aggcacttct tcaagcgctg ctggttgttc
    67141 aaaaaataaa ttcattttaa aagttttgct acttaaatta gtgagcgaga atgttaatgt
    67201 tttacgatga acatgacccg gataaaatgg tgtaaattca acacttccgt tcgtaatagg
    67261 aacgctatta agagcacagg aaaacgaatg agcgtactca tcatcaacac cgatatcagg
    67321 aatttcagcg ccccctttaa gcgtcgacat aaaccatgct agcgttgtaa tagtaagcgc
    67381 gaccgttgaa acagcggtac ctaaaaatgt caaaaatagt ctaaattttc gacttggtaa
    67441 tttcgctact ttcttgatct tgttttgact atccatgttc actttctctt aatagccttt
    67501 tcctgtaaaa aaagctacct ctctgttagg tagctggcta tctcgtttcc aagatcctat
    67561 agctttgcgt cgctagattg ctctagtttt gcctttttct agcattatta tattcataaa
    67621 tatgaataat gtcaataatt tttgtagatt tatcggtatt tttctaagaa ataaaactta
    67681 ttcaacttcg ttcattattc aaaacattaa aaaatatcgt taatatgata tgttttttaa
    67741 tactttaaag tagtaattgg cacaacataa acaccatctt cacgtttata agcataacga
    67801 cttaatccgc aaataacgac taaaatgtct ggtcctttga ccccttctta ttctaacgct
    67861 tttttatttt cactaactta ttagcggctt catcaacatt gccggtttta ttttaagggt
    67921 ccattcgctc acattttggg cttctgttag gtcatatttt gagtttcttt tcgtttatat
    67981 ttcacattta attgatttca actatcccaa ttattttttt ttaagtgtta ctacaactaa
    68041 tatttttcgt atttttttca gttgcactta aatttttacg catttaaata aatagtcgcg
    68101 cattctttta taaaatgatt attcgacaat cattacttac ttatttcaaa atcacaattt
    68161 ataatttcta aagtaagggc ttcacgatat ttccaaaaat agtttttaca tttgctaaga
    68221 tccaatatat agttcttttg ttgtatgtgt gtctacattt agggtatcag cacataagcg
    68281 atattatttt tgtccactta ttttatgata catttcaaat aattctttta aagacacatc
    68341 aactcctgct tcatgaagtt ttttctttct tttattaaat agttttttaa tcattttatt
    68401 tgcatctcta ccataatgaa tttcgttatg acaattgcta caaagcgaaa ctatgttttc
    68461 tggaatatca attgatttat caaatttata atgatatgcg agcggaatta aatgatggac
    68521 ttcagtatac ggcattccgt tgctttttct caaaaaacat ttatgatttg aattcgcttc
    68581 acacttatag ttagcatttt taagagcaat tgcagccatc tttacatcac gaggataata
    68641 tagtccccct cccgtcccct tcttcaaact aactttatca accgggtgcg taatatagat
    68701 atatgtagat tctttttctt ttgataactt tttatttaat ttattatcag cttcttcatt
    68761 tagttctttt gtttggatta cattgtattt ttcacgaatt gagtacaaaa aaatcatgaa
    68821 gtcatgatta tctttttttg aaaaatgttt atctttgtat ttaatcagcg ctttttgcgc
    68881 attttcaaaa gttttactac ttttaccaat taaatactct tcaaagtatt gtaaatcttc
    68941 aagtaaaaaa caaggtatca taatagatgg gttgtatagg tacataattt tatatttaaa
    69001 catgccagaa aacaacgatt ctaaatcttt aaattccgta agttctctcg tttctctaat
    69061 taattttgcc aatgcgattt tgatgtcttc aaatgcttta ttaacactac aattaaaatg
    69121 ttgttttgtc gctctatatt taatctcttt atcttctcca tgagttccat accaaagtcc
    69181 aaactttgaa gaatttgcgc cacgaatctc acctaactct tttgtaccca cttccaccaa
    69241 ataacaaaac gacttattgt ctgatcccca tccaactaca tattcctttt tatcggtcat
    69301 tgcttcaatg tactctggtg taaaatgtgt tactaaataa cgccaattct cccgagtttt
    69361 tttgcttatt tttttatttt ttttcatatt ctatctatta atgtggaatt ttccggcaat
    69421 taaaagcacc ttttcttttt tatttttggt tgtttttaag gacacataaa tttttacaca
    69481 attattatta ataactaaat tttttgttta ttttaaatta acccacctct ctattttaca
    69541 ttgtagcacg caaacatgat tgttcttata tattaattat tatccattta aacacttcca
    69601 tatttctcaa atttttccct caagtttgtt tgcctcaaca gcaggataaa ttagtcaaaa
    69661 tttttcctta cacttttctt ttaaaattgc tttataatca ttttgtattc ctttctaaaa
    69721 atacatcttt tttaatattt ttaaaaacta atagtcctta tagaataaaa tatgtgtttg
    69781 ctatattaat aaaaaagatt atctattgaa gagcgttcta taattacctt ataaataatg
    69841 agtatattat tttcttaaaa tcaaactaac aatcgtctcc gcgtgagcgg tccgcggaaa
    69901 catgtcaaca cattcaatat gttcaacatt atacttc
//
LOCUS       DAOSLP010000002        57327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_640_length_57327_cov_10.275684, whole genome shotgun sequence.
ACCESSION   DAOSLP010000002 DAOSLP010000000
VERSION     DAOSLP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 57327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57327
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_640_length_57327_cov_10.275684"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1633)
                     /gene="argS"
                     /locus_tag="PK460_00305"
     CDS             complement(14..1633)
                     /gene="argS"
                     /locus_tag="PK460_00305"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOE53578.1"
                     /translation="MIDIRLQELIKDATVKLGLDIDQDTIVIETSKDSAHGDYATNIA
                     MQLARPLRKNPREVATLLINAMDQTPFEKIEIAGPGFINFFLKPETLSKYIATILKEG
                     SNFGNGSKKDTKVNIEFVSANPTGKLHLGHARGAAVGDTLARLFIKSGYEVIREFYVN
                     DAGVQIDNLGKSLYVRYLNLFGIEKELPKDAYHSTDLIAIAESLKAEIGDKYVKESKE
                     GLEYFKLEGTNKFLDIIKKDLQNFDVVFDVYSYETKVRAGNTIPKLLEKLAPFTYEED
                     GATFLKTSDFGDDKDRVIIKSNGDFTYFLPDIAYHLNKLSRGSSLLIDVLGADHHGYI
                     GRMKAALQMFNYPADTLEVQIIQLVRIIQDGKELKMSKRTGNAITLEELCEEVGVDAA
                     RYFFLAKSASTHFDFDLDLATESSSANPVYYAQYAHARLSAVLARGEKFSLDPSGEGL
                     KEAAELNLIKQLIAYPSAVSDATMTREPYKIANYIQKLASLVHSFYTEVRILDESNVQ
                     LTSARLGLSIAAQIVLKDALTLLGVSAPQTM"
     gene            complement(1771..3801)
                     /locus_tag="PK460_00310"
     CDS             complement(1771..3801)
                     /locus_tag="PK460_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOE53579.1"
                     /translation="MSANIKTFGLGVFNKSPNVEYKVYENGLYLGNSTLSEHLLLVEI
                     IDKEQTTFKLHPDTRTIAEFACYKSNMTEVTSWGNNLKTIGYGAFMQSKLTAITLPNS
                     LETIADSAFKQTKLTSLNFGTGLKAIGYEAFSNCPDLADVTLPNSLERIDSSVFYGTS
                     VTYTDYENGRYLGSGDAGETANPYFALMEVITKTMTTFTVHADTHLIVPGVFYGSSSL
                     ESVEIKEKVSFIGSGAFENCIALTSLSVAANNTHFYSPSNKAILEKDTDRLLFGLNGG
                     FIPEDTKIIGEKAFYYRKFTEPLVIPSSVTTIEDYAFYQTNLTAIVIPDSVTKIGKSV
                     FQYSNSLTSVTLSKNLKVIEDWTFSGCKILETLTIPDSVTMIKEWAFSNCEMLANVTY
                     SANLAFIGDVAFSSCVALTTLPASESLRVFGSGSFRGCESLTSITIPKNVRYISSGAF
                     ADCTALTTLNVVSENNHYDSRENCNGIIETATNTLMVAVKDTVIVESIEALGAEVFSG
                     IDIQSITIPASITKIGNWAFSDCGKLETVVIEGELTMINAGTFSHCTSLTSVNIPETV
                     TEIGGSAFYNCKVLPEIKLPANLEKIGSWCFGRCTILPQIVIPRSVTNIARSAFSSCA
                     NLTIYAEATALPDGWHADWNDGNRPVYWYSETANYDGSHWRYVDGVPTVWAE"
     gene            complement(3844..4320)
                     /locus_tag="PK460_00315"
     CDS             complement(3844..4320)
                     /locus_tag="PK460_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOE53580.1"
                     /translation="MPKYDGATPTRPDDDTYTYSFNGWDPEPVPVVADATYVAKYKTA
                     LKEVVDPRDFVFADTGEGYTLCGYYGSASRVVIPETYNDKPVVSLEKWAFFNLDNLVS
                     VTLPNNLRKIGEDVFADCDNLKHVDFGNGVQEIGFMAFDHCPSLEAISLPDSVETI"
     gene            complement(4697..5812)
                     /gene="ychF"
                     /locus_tag="PK460_00320"
     CDS             complement(4697..5812)
                     /gene="ychF"
                     /locus_tag="PK460_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOE53581.1"
                     /translation="MGLSVGIIGLPNVGKSTLFNALTASKVEAANYPFATINPNKGTV
                     RVLDPRVTKLAEYYEPEKTIYATIEFNDIAGLVRGANRGEGLGNQFLGHIRECDVILE
                     VVRCFSGEEIIHVEQSVDAKRDIEIINLELILKDLEVLYRRKDKVETKARVNKDKDSV
                     FELEIINKLIPALENEVPARLVTTLTQAETDYVKLHFGLLTIKPLIYVANISDTEYAT
                     YEDNVEYQKVKAIAKREGAELVAISAEIEAEIATLSSADQELYLAELGINESGLNKVI
                     NVAYKTLGLSSFFTTGKDEVRAWTFKDGTSAQKCAGIIHSDFERGFIRAEVYSYNDFE
                     KYGSEQKIKEAGRVRSEGKDYVMRDGDIVFFKFNVRK"
     gene            complement(5916..6113)
                     /locus_tag="PK460_00325"
     CDS             complement(5916..6113)
                     /locus_tag="PK460_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOE53582.1"
                     /translation="MKGTIKQFNQERGYGFIRAEDGTDVFFHYSSLVMDGFKTITAGE
                     KVEFTLEQSDRGPRASNIVKI"
     gene            complement(6228..8930)
                     /locus_tag="PK460_00330"
     CDS             complement(6228..8930)
                     /locus_tag="PK460_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE53583.1"
                     /translation="MISIKNLECVLGGERILNIASFEINAPGLYVILGPSGCGKTTLL
                     NIITGLNSEYTGSVELFNRTYRHASKKETTTFRSRYLSAFFQHSVFIEELTMRQNISL
                     VALNHTKSLENLVNSKVEKLAKRLNIEKVLNQKVKTLSGGEKTRGAIARTLLKEVPLY
                     IFDEPTAALDRENAVIVMDLIREYSKTNIIILVTHDESLANEYADTIFTMEYGEFKSV
                     NIIRNNELRVAKALPEIPYAKNSDYIARGLLKAKKARHFFTGGAVNLGLVGLGLSFML
                     VNAINYKLVNTFKGEFTNETVYVQKKFAPEINVLKSVSHEEIALNISAEKELGSFYLN
                     NFNDFFPHQNELSFNHLGIRHVLPSFHIGLLNESLYINEIHEQLYPYVTNLAIDEIGL
                     VLPFDDFKLIQNVLRIPMRQTPSELGVYLENNNFYVTLEVTNNNWDYFDEQIFRLRTV
                     TLGREAQIIMGEPSFIPFIFEEKMMFPSSLNLTKIEEYPWTLKKINYMLLNNAFDYLW
                     QSLNYSNYVFCTANNDYFQYIYNKTYLQNRLLIFESPPHFNSMFKSYSSESEAEFYTF
                     SNGLTFIPEMMMLGFAHNFFITQDITLLEAAMWADINDHSAPSSYLELPEGILNFAIQ
                     YNGFSSFNYRNANTPLRIDELIISSGLANKLGLSKDAHNTELHIGALVAIAEASEGYL
                     KEYETIKVSIKRVIDNDEVVIYHHPLWAYLLYKDVFNVFPFALEITGAIYQSVTNEDL
                     KNLQKHFSVSYPYKDFSVLVADTLNELERYTFLASVGAFLLAALITFMTIYLLVAETS
                     DQFSGLFLVGYNEEAIHGVISRYILRFLGVIILLAIVQLFILSFVVEVVLMKYLETSF
                     RYVFNPKPYLLVLAFATGLLIFLLTYFKFQHEKLDFLAFSKRDL"
     gene            complement(8923..10179)
                     /locus_tag="PK460_00335"
     CDS             complement(8923..10179)
                     /locus_tag="PK460_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53584.1"
                     /translation="MKIITLFKVIANLYKRNIKKNRLMVIFMIFTNITLMLFLVFFSS
                     SETLAQTLAEGYLEYLSVDVALYESQPTNNPLINVKSYRRPSIDYLNSIMKVTESFVI
                     RPDYSPLLYEKEIMVLGKEIPNVLITIIGENTNEIGINRAFYNELNKLVSIKDDEMLL
                     HFYIKTTIFIDDEEVLIDYYNQFPITYIYDEPTYFSVPKLYISQNTIDATLGNIFLKP
                     GLTLNNYLLNLNPEHELSNYRFKCHFKSAAQKDKFMHIVNEVSNNESGIEVSGDHINK
                     IESFNALYDYLSILMKLFLAFVIIGSVVIYVVIAHTALLTILRQMALFAVLGASKKQI
                     YEIYLFLIIINFLLSSVSFLLLPSLLPVVSFILHKLMGVYVVLELNYTMLVLMLSADL
                     LTLLILITIIFLINMRRPLLYLLIDD"
     gene            complement(10157..10735)
                     /locus_tag="PK460_00340"
     CDS             complement(10157..10735)
                     /locus_tag="PK460_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE53585.1"
                     /translation="MFSNLSYTFKSGKIYVIVGYSGSGKSTFLHIVANYISKNSGKIK
                     YKNKLITKNDVTLVLQHPYFLGDLRIIDNLKISSLFRHLNNRKIRQKYTKALQISHIL
                     QRRANVCSGGEKARANILRGIIERKHILLIDEPTAHLDFKNSQIVAKLLSELSANKIV
                     IVTTHQPSLFKSGNTIFLRIKNGVFHEDNNII"
     gene            complement(10809..11027)
                     /locus_tag="PK460_00345"
     CDS             complement(10809..11027)
                     /locus_tag="PK460_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOE53586.1"
                     /translation="MALIKVDLYDEIEMKKPHPCIHKSHRFQVVMLGADLKLQCQGCG
                     NIVLISRIKFNQKFKKHIASHETIIFTK"
     gene            complement(11027..12631)
                     /locus_tag="PK460_00350"
     CDS             complement(11027..12631)
                     /locus_tag="PK460_00350"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOE53587.1"
                     /translation="MKNTLHILEVAKKVGLSPADVILYGEDKAKIKSPFTDINPQGKL
                     ILVTAITPTPFGEGKTTMSIALQDAMWKTGTKSLLCLREPSLGPVFGIKGGATGAGRA
                     KLYPSDDINLHFTGDLHALTSSINLIAAVIDNHIFQGNALNLDPKRITWKRSLDMNDR
                     GLRKVRINQGTGNGTPRCEEFQITVASELMAIMCLASDPDDFIRRVKDIIIGYTYEGE
                     IVRLAALEIEDAIMHLMKQALLPNLVQTLEGNPVLVHGGPFANIAHGCNSIIATKYAL
                     SKAPVVITEAGFASELGAEKFFNIKCRTANLKPNLAVVVATIKALKMHGGVPMSAIYE
                     ENTDALAKGFENLVQHYENLSLFGVKVLVALNLFDNDHKSEVKLFEKEMQKLGYDYAL
                     VSSVSKGSEGGIEFANKVKALLAEETKFEFLYDTEKEVEEKIEIICKKLYRASGVKYS
                     KRAQRELAVIKEQGFAKLPVCMAKTPLSFSDNPNLKNAPRNFNITIREFRLSAGAGFI
                     VALTGKVLTMPGLPKVPAAVNMKEVR"
     gene            complement(12628..13509)
                     /gene="miaA"
                     /locus_tag="PK460_00355"
     CDS             complement(12628..13509)
                     /gene="miaA"
                     /locus_tag="PK460_00355"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOE53588.1"
                     /translation="MIFVIGGVTASGKSDLAFRFAKAINGIIINADAFAFYKELNIGT
                     AKPTKEELAMLPTFLFNNISLDERFSIYNYQKEVRIILDKYLAKKIPIVLVGGSGLYI
                     RAALYNYNLQPEKDLKIVDDPNISNTSLHKELEVLDKEAAAKIHPNNRKRVLRALALI
                     KTHAQTKTALEEVTSSEPLYPYIMICLDKENEMLHRDIETRVKLMFDKGLREEAMTLS
                     KKYPRNAQGMQAIGYKEIVQNPNATDEELINLIALRTRQYAKRQRTFFRNQFAAKWFT
                     DKEAAFNYLRGKYEDSL"
     gene            complement(13506..15260)
                     /gene="mutL"
                     /locus_tag="PK460_00360"
     CDS             complement(13506..15260)
                     /gene="mutL"
                     /locus_tag="PK460_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOE53589.1"
                     /translation="MAKIILLDNELANQIAAGEVIERPSSVVKELVENSIDAGATKID
                     VFINEAGRKAIIVQDNGSGIPSDELALAFSRHATSKIHSTFNLFRIRTLGFRGEALPS
                     IASIAKVQVETAEKGKPGALAKIENGDIKIEPSAVSSGTKITVSELFYNTPARLKYLK
                     RDYTENAVTLDVVTKIALAHPDVSFSLTFDDKLRFRTSGRNNLEETILNIYGLTYVKN
                     LLPISVKTNDFTITGFLGNTNLVRSNRYGIITNLNGRNVYMPKVQSTIIEAYHGFIAP
                     TRYPFVILNLQIEPELVDVNVHPTKKEVRFSKEEDLRNVLLKEIPLALRKEAFIPKDP
                     HQKVQPLSSTTNEEPRAEQLIFDLPFGTETSELKISDSHHQEAIRKPITKTISELENS
                     FVNPVAALHNTYIICEVNDGGFWLIDQHAAHERINYEKFQKVLNMDLKTRPLLVPELL
                     SFSHSEIALFTPEVFTILKDVGIEAELFGPNTLRVTAVPSWAGEYDERIYIQDIIDAV
                     ITDKRLNQELLRTNAIATMACKASIKANHRLSIMEMQHLIKELLACDNPFSCPHGRPT
                     IIKFTKYELEKMFKRTGT"
     gene            complement(15260..17791)
                     /gene="mutS"
                     /locus_tag="PK460_00365"
     CDS             complement(15260..17791)
                     /gene="mutS"
                     /locus_tag="PK460_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOE53590.1"
                     /translation="MGKEKKKIVYTPMIEQYLEIKRENPGILIMYRLGDFYEFFFEDA
                     EIVSKELQLVLTKRAAGNNRKIPMCGIPHHAYGPYLAKLIDAGHKVGIVEQMEDPSSA
                     KGIVKREIIQIVTPGSIVDFEDSDNNFITAVDAYADAFTVSYADVSTGDIFVTTLPRE
                     DTSLVSYLNGLNTREIVVSPQFSDKLLGVIKTNINLVISFEENTKIDEEYEDLFSDVK
                     DEREYLIAARLVSYLTRTKKRQLSYFKAITVTKPQSFLQIDAFSRLNLELTRTIRNET
                     KYGSLFWLLDETTTAMGRRKLKDFIIRPSRDYDEIIRRQNIVNTFIVNFVTRAEVKEH
                     FVSIYDLERIVARISFGSANPRDLLCLKTSLQALEKARNLLGKIREPEISELYDKLAS
                     NIPLITLLEKAISEDAPLQITGGEIFKYGYYEALDELIDLAHGGKKWISAFEEKERVR
                     TGIKNLKIGYNRVFGYYIEVSNSNLDQVKDEFGYIRKQTTKNGERFINEALKEQETLI
                     LSAEERRQQLERELFGALLNELKKSTRLLQQSADVVSEIDVFISLAEVAVANNYVRPI
                     FNDARVVDIKEARHPVLSKLLKDHAFVANDIELDEATDIVIITGPNMGGKSTLMRQLA
                     LIVIMAQIGSFVPAKSANLMIFDQIFTRIGASDDLISGQSTFMVEMIEANYALRKATP
                     NSLLLFDEIGRGTSTYDGMALAYAMLEYIANKLKVKTLFSTHYHEITAIDEHYPNIKN
                     IHVEVKEVENNVTFLYKIKPGPMNKSYGINVARLAGLPLEILSNAQEHLQTFVANNSG
                     GNPTKTEYVPLKPLWYQQLENLNIDEMTPLDALILLKKIKDEGFK"
     gene            complement(17823..18134)
                     /locus_tag="PK460_00370"
     CDS             complement(17823..18134)
                     /locus_tag="PK460_00370"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOE53591.1"
                     /translation="MNKVEIALNALTTELANDKRVVEFKKVKALIESDAYLKNVEVRL
                     KELQRLMTQNAFNEEKHNEYKREYLRLKNNYETHPYLINYNSLLSEIEDLLYSLKTVI
                     E"
     gene            complement(18127..19563)
                     /gene="miaB"
                     /locus_tag="PK460_00375"
     CDS             complement(18127..19563)
                     /gene="miaB"
                     /locus_tag="PK460_00375"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOE53592.1"
                     /translation="MSKTLLNNLPSFTKARRRYPSIVQNLKIEVPSIVSAKLSGKFFL
                     IKTFGCQANVRDEESMAGLLSMANMRRTEEEKDASVIIINTCAVREGAEQKIFGQVGN
                     LKGLKTNNKDLIVIVAGCVVQQEESLAKLRKTFPYVDIFMGTNAIHKLLDLIALRITE
                     NKKVVDVPSDDADVYENIPVQRIAPHKAFVNITYGCDKFCTYCIVPYTRGKERSRLPA
                     DILRECQILVDSGYQEITLVGQNVNAYGIDLKDGTTFSYILEEVAKLGIPRLRFITSH
                     PWNFDDEMIDIIAKYPNIMRKIHLPLQSGSDEILKLMGRRYTVKDYLELVTKLRARMP
                     DISLTTDIIVGFPNETEQQFLETLKVVEKVNYENAYTFIYSPRKGTPAALMEDNVSRE
                     EKGERFNKLKALVDSQTEANAALYVGNEYLILVDGYSKKDTSVLSGYTESDRVVHFVG
                     TPDLIGKIVKVKITESRVYSFVGELVNE"
     gene            complement(19556..20353)
                     /locus_tag="PK460_00380"
     CDS             complement(19556..20353)
                     /locus_tag="PK460_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOE53593.1"
                     /translation="MVKILFIGDIVGKIGRETLAHFLPILKREHDFDLIIANGENTTH
                     GRGLTKKHYEELVNLGIDVITLGNHFDDRPELKYYITEVHNLVRPLNLIENYPGEGSV
                     VVTTKKGDKVRVTNVLGQAFMKEIITSPYANLKMLNADTEKMIHIVDYHAEATAEKMT
                     IGWEFDGAVSAVLGTHTHVQTRDARILPKGTAYISDVGMTGAYNGIIGADRERVIERT
                     WFDKQVPFTYEYKDKEAQFSAVILEINKHTFKTEKITPLYYLWRRDE"
     gene            complement(20356..21906)
                     /gene="rny"
                     /locus_tag="PK460_00385"
     CDS             complement(20356..21906)
                     /gene="rny"
                     /locus_tag="PK460_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOE53594.1"
                     /translation="MEVLYYALFFLVGLGLGIGLFFLIPAFKAKQAFAKAQKLTKEAE
                     IKAEHIVKNAELDAKQIIYEGKQELEQAGKERRAQIRELERLLNDREKGIERRDQLLH
                     SKEQSLDARHENLSVKIKEADELKKDLQIKIDSILGELETVAQMTVSEARDELYHRVD
                     AKLEKEIALYIKQKEEEAELQVNEQARDILANTIARYSQEVTTERTVSTVELPNDEMK
                     GRIIGREGRNIRTLEQLLGVDILIDDTPEVITVSCFDPIRRETARRTIELLVKDGRIQ
                     PSRIEEVVEKATQELDEIIIKTGKDALAELGIPNVHKDMIPFIGRLKYRTSYGQSALK
                     HSIEVAHLCGIMAAELGLDITRAKRAGLFHDIGKSADFELEGSHVEVGARLAKKFNEP
                     DYVIDAIESHHGDKESRFIFSQLVIAADTLSAARPGARTETLENYIKRLEKLEEICKS
                     YDGVQTSFAMQSGREVRVMVHPDKVDDIMVNKLVRDIRERIENEMTYPGQIKVSVIRE
                     YRASDVAK"
     gene            complement(22200..23201)
                     /gene="recA"
                     /locus_tag="PK460_00390"
     CDS             complement(22200..23201)
                     /gene="recA"
                     /locus_tag="PK460_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOE53595.1"
                     /translation="MSKKKDANILQETLKEIEKSFGKGAIMKLGERPVINVDVIPSGS
                     LLIDEALGVGGYPKGRIIEIFGPESSGKTTLALHAIAECQKKGGQAAFVDAEHAIDPI
                     YAKNLGINIDELLLAQPDSGEQALEIVVRLAESGVIDLIVVDSVAALVPEAELSGEMG
                     DNQVGLQARLMSKAMRKLAGVLNKAECTVIFINQLREKVGIIYGNPEVTSGGRALKFY
                     SSIRIDIRRGEAIKQGSKLIGNQAKIRIVKNKVAPPFAACGIDIIYGKGISKIGEILE
                     LAVNRGFIKKSGSWYEHDGLKIGQGREAAQNYILERPELLADLEEKIKTGVVATETK"
     gene            complement(23235..23708)
                     /locus_tag="PK460_00395"
     CDS             complement(23235..23708)
                     /locus_tag="PK460_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOE53596.1"
                     /translation="MTTQLLINELIDKNLTIGSVESFTGGLFAATLVNYPGVSKTYKG
                     TIVAYSNDVKERLLNIKKEDLKKFSAVSSVVALDLARHGQKALGVDFCFGFTGDAGPT
                     ASGQSGEVGDVYIALAFANDEIVEHFKFKGDRLKVRTDAVNAALKMLARVLKNFE"
     gene            complement(23686..24324)
                     /gene="pgsA"
                     /locus_tag="PK460_00400"
     CDS             complement(23686..24324)
                     /gene="pgsA"
                     /locus_tag="PK460_00400"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOE53597.1"
                     /translation="MNLPNKLTVARMILVILLIAFLLIPPTWLGNEPIILFSNVYLRY
                     FIAAIVFIVAAITDYFDGYLARKFNLITNFGKFMDPIADKMLVNSMLIILTVPHQAFV
                     GQISVPVIFVVLMIARDTVVDGFRLVAANQNKVLAANIFGKAKTVLQMIAIVMFLFND
                     WPFSHQMQSDSFPIVSMIVIGAATFVSLLSGFIYIFQNISVLKETHDDTTAH"
     gene            complement(24349..24682)
                     /gene="rnpB"
                     /locus_tag="PK460_00405"
     ncRNA           complement(24349..24682)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK460_00405"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(24687..25037)
                     /locus_tag="PK460_00410"
     CDS             complement(24687..25037)
                     /locus_tag="PK460_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOE53598.1"
                     /translation="MAEKRLLDADKILKKKFKAKSGGYDALEVDEFFDLVRNDYESML
                     EIEKELELLRLKNETQQAKIVNLEAQYIQYKKKVEELERLISKGGTAMENLRKIDKYE
                     RQLWKMGIDPSKLK"
     gene            25129..25854
                     /locus_tag="PK460_00415"
     CDS             25129..25854
                     /locus_tag="PK460_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOE53599.1"
                     /translation="MKKLAKATRGRKQLIFLDLEGTQFSHELIAIGAIKATIKTDGTI
                     KKLYPGFKHYVLATQNVGNYIEKLTGITDDLLAREGIPFDKILLMLKKYVGTNLSKTK
                     FVTFGAHDIRILRQSLAHTPEAHHTFIKTIAKNHIDLSAYINEYIKDEKNNTLSLVNL
                     CKLFGVEPVEPAHDPLNDALMLAYLYSELFKKTDILIERYGEVLLNMNNIPRPIKRML
                     NKIKAGESVNIDDLQEYLRDEIV"
     gene            25854..26444
                     /gene="recU"
                     /locus_tag="PK460_00420"
     CDS             25854..26444
                     /gene="recU"
                     /locus_tag="PK460_00420"
                     /inference="COORDINATES: protein motif:HMM:NF002581.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HOE53600.1"
                     /translation="MINYPNGSKPKKQTTKVPSKRKNITKSAANRGMALEKLISAAND
                     YYLMHDIALFTKRPTPINVVKIDYAKGAKITNAYFEKQSTTDFNGVYKNRYFDFEAKS
                     TRLKTSFPLNNIYEHQITHLENVIRHGGIAFFIVEFTAFDEYYVLSAADVIDFYRNGD
                     RKSIPYASFKEKGVLVRLGLNPVLDYLPAVIKLFNL"
     gene            complement(26439..27074)
                     /gene="nth"
                     /locus_tag="PK460_00425"
     CDS             complement(26439..27074)
                     /gene="nth"
                     /locus_tag="PK460_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOE53601.1"
                     /translation="MTRTNKERILAFLDKEYSDAKGELEYTKDYELLIAVVLSAQSTD
                     VAVNNVTRKLFAKYPTFESLLNVKEAQFAAYFKTLGLYRNKAKHVYNLIHLLNEHHNG
                     VVPSAKEELLKLPGVGVKTANVVRAELFNIPEIAVDTHVSRIARRLGFAKFSDDVTII
                     EAKLRKVLPKERYIKTHHQMIHFGRYFCKAQSPLCKKCPLVDICKEPNKKL"
     gene            complement(27058..27675)
                     /locus_tag="PK460_00430"
     CDS             complement(27058..27675)
                     /locus_tag="PK460_00430"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOE53602.1"
                     /translation="MLFEYYKELRINETELVIILFIEHLQQKETEFITPDLINLQSNI
                     DIKVIDEAMVRLVSKGLIEFTTRNGKMVTSLNPLRNKLQELFVLDYEKNKENSTVEFS
                     SQVEEVYAFIQNAFGRSLSAVELQTINDWFNYGYNITMIKEALDQTMKRKRYNIRAID
                     KALLKLSTAVDYALEGKSAQSDSYRKNIKDTLEEVNKKAQDDENE"
     gene            complement(27698..29485)
                     /gene="glmS"
                     /locus_tag="PK460_00435"
     CDS             complement(27698..29485)
                     /gene="glmS"
                     /locus_tag="PK460_00435"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOE53603.1"
                     /translation="MSGFIGRVGNSCVKEKLLSALNLLQFKDDDSTGVLFCNKEGQHL
                     YRVVGKVDELVTLIPEEVDGYMGIAHTRWSTYMHQVLENVYPLSSENGYVTLVVNGLI
                     DNIQMIKRRLVKRGYSFRSSSAVEVVANLIEDGAVENEDPLRTLHRAMQQLEGDYALL
                     AVFNGNCKKIFFAKNGVPLIIGKNHDGMVICTDYTPIADQADEYYYPNDGELGYLTSD
                     NMVLYDGKLKQRKPSFTEVSVSEKTLELGDYPHYMLKEIEEAPGVIRRLINHYFDGTK
                     YRFDDRLIKRIIDAGTIVFIAAGTSYNAALVGQRYLRNFNKKVDVFIASEWIFYPYQS
                     GEDTLYVLISQSGETADILKCLRTIRAYGADVLAITNVEVSTLYQSADYRLLLHAGTE
                     VSVASTKAYIAQITLLSLLYARLVNKITTIAALERVIEALYNIISRRDTIKAIADQIY
                     KSGDVFFLGRGFDYDVAVEAQLKLKETSYIHAEAYPGGEMLHGPIALIEENTPVIFFI
                     SDSLTVAATREVIREMRDRKANVIVCSLAPFNHDGDSFIISNPIKGYHSPITFAVFAQ
                     YLAYYCAVNLGRDVDKPRNLTKAVKDRSV"
     gene            complement(29540..30202)
                     /locus_tag="PK460_00440"
     CDS             complement(29540..30202)
                     /locus_tag="PK460_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53604.1"
                     /translation="MLKSYLEFFDEIANIFNKHGFKLYMVGGTSRDFLLNYPVDDYDF
                     ATDATPTEMHEFLPDANYRFEKFGTVSLKYEDEHVEITTLREEGEYIDSRHPAYIKFV
                     TDPAKDYIRRDFTINALYIDENYRVLDFCDGLKDLDTNTLRVIGNPYTRFAEDPLRII
                     RALRFVAKFKLNIDPLTENAIRDLAHLVKKLNPEKVKAEIRKVGEQEQNIFKKLMNMY
                     KL"
     gene            complement(30224..30496)
                     /locus_tag="PK460_00445"
     CDS             complement(30224..30496)
                     /locus_tag="PK460_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE53605.1"
                     /translation="MNKAEIIAAIADELMITKRSVEEIINLFCDTVIETLAKGEDVKL
                     TGFGTFQTRERKERRGVSPQTGKEIVIPSGRTIVFKPSKLLKEKVQ"
     gene            complement(30653..31960)
                     /gene="der"
                     /locus_tag="PK460_00450"
     CDS             complement(30653..31960)
                     /gene="der"
                     /locus_tag="PK460_00450"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOE53606.1"
                     /translation="MALPVVAIVGTPNVGKSTVFNRIIGERRAIIHDERGVTRDRLYG
                     SAEWLTRRFNLIDTGGIEIAKTAFQEQIRAQVEIAIEEADVIVYVGDGPLGVTTDDRE
                     IANLLYRANKPVIVAVNKIDGIEHHHYIHDFYSLGLGEPIAISGEHGIGIGDLLDEII
                     KVLPEQKDDYEEDVISFAVIGQPNVGKSSLVNAILNRERVIVTNVEGTTRDAIDTPFT
                     RNNKNYVVIDTAGLKKRGKIYESVDRYAALRAFSAIAKADIILFVIDAVVGIREQDKH
                     VVGYAVDEKKAIIIAVNKWDAITKDDKTMNEWTNKIRNEFKFVEYAPIVFVSALTKQR
                     VDQIFDQIDYVNEAYRRRVETSLLNEVIHRAQLYNDPPVFNGGRLKVYYANQVSTKPP
                     TFVFFVNNPAFLHFSYERYLENQIRDSFPFDGTPLKFIFRERK"
     gene            complement(31960..32634)
                     /gene="cmk"
                     /locus_tag="PK460_00455"
     CDS             complement(31960..32634)
                     /gene="cmk"
                     /locus_tag="PK460_00455"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOE53607.1"
                     /translation="MGINNIAIAIDGPAAAGKSTIAKKVANHYNFVYIDTGAMYRAFT
                     WYVLKRGVDPKDETASNSLIPEVTIRLTADYKVFVNGEDVSKAIREPSVSGNVSYIAS
                     YPKIRLFLTEQQREMAKVNSVVMDGRDIGTYVLPDAFVKIFQTASVAVRAKRRYLENL
                     EKGIATSLEDIEKELAMRDHIDSTRAMAPLKKADDAIELDTDNLSIDEVVAEIIKIVD
                     KKLGVK"
     gene            complement(32621..32941)
                     /locus_tag="PK460_00460"
     CDS             complement(32621..32941)
                     /locus_tag="PK460_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53608.1"
                     /translation="MAKRRESKTRVEYWAKYRESITNDFEVEQKMRESRKKQAENTPI
                     KKTETPPLTKAEALLSEYERKNENKESAVKKGPIGILYGLAIIVLLLIIAGIIFMILI
                     YGNK"
     gene            33066..33260
                     /locus_tag="PK460_00465"
     CDS             33066..33260
                     /locus_tag="PK460_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOE53609.1"
                     /translation="MAKKVKLDKDLCISCGVCNAVCPSLFDWDDDGKLKALVEEVPAD
                     LEDAAAEAEASCPTGAITVE"
     gene            complement(33315..33974)
                     /locus_tag="PK460_00470"
     CDS             complement(33315..33974)
                     /locus_tag="PK460_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOE53610.1"
                     /translation="MEKEKNYTNCQNDNLETNAQQKCKRRRTVTVAGTAIFAAISVLL
                     YITKVPPFVFPIFPLVNFLEINFSEIPALIAGFAYGPLSGFLVLLVRFLVKLPMSSTA
                     MVGEVADIIYSSSFVLTATIIYKYRRTFKGALLGLGIAFIAQILVSSLANYFVIYHLY
                     NEMFFNGKMAMILSNKYGAFTPEQFILWVIPFNFIKNTIIGGLTLVVYKRISRFINQF
                     Y"
     regulatory      complement(34013..34125)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(34165..34971)
                     /locus_tag="PK460_00475"
     CDS             complement(34165..34971)
                     /locus_tag="PK460_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOE53611.1"
                     /translation="MKNEPKNNLLKEHLSDLISKYSQSNVLEELAKAHTNRPIQMLPI
                     DLIVDNNFIKKITLSRKKIARFVENYQRNILLEPLVVRAKGDKYEVIIGRRRLAAARL
                     TEIQEIPVIVMNYNDEETLLILLAKARDQLGTNVLELAYIFSVLSTKHGYSHQALADL
                     TFRSRSQVTNILRLLKLEPEVIRALNNEQITFGHARALISFDREKSLEYLELIRVNNL
                     SVRELEALIKAPNTATYNGKDYFVIQMKNKLTIVFNTEEEALAFKNKHFK"
     gene            complement(34949..35728)
                     /locus_tag="PK460_00480"
     CDS             complement(34949..35728)
                     /locus_tag="PK460_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOE53612.1"
                     /translation="MAKIIAIANQKGGVGKTTTAINLSASFAHFGRKVLLIDMDPQGN
                     SSRGLGLDPATIKNTIYHTFFKGININKAIYRTPLKSLDIIGANLLLARTDVEISTIP
                     QHPFTVLQSKIKEIDRRYDYIFIDCPPSLGLLSTNALVAANSVIIPVQCEYFALEAVA
                     QVLATIKQVQASHNSELKIEGFLLTMYDERTRLGIEISQQIRGLFKESTFMTPIPRNI
                     ALAEASAKMLPTILYKPTSKGSLAYLNVAKEIMENEERAEE"
     gene            complement(35762..36463)
                     /gene="rsmG"
                     /locus_tag="PK460_00485"
     CDS             complement(35762..36463)
                     /gene="rsmG"
                     /locus_tag="PK460_00485"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOE53613.1"
                     /translation="MKNNPFLQYLDEHFSENAEVLKVQFAKYAALIEEHNKVQNLTAI
                     APSEYEEKHFLDCLLLRETYNFRNETLLDIGSGAGFPGIVLAIAYPELQITLLEPIKK
                     RCNFLSTVVKELELTNVEVVNERAEDYAASKRGEYDLVTSRAVSSLSIMLELSVPFLR
                     INGSALMMKGRNYNAELSDAENAIYTLKVEVGKTDLHFLPSDGSMRVNARFIKKGSTP
                     KKYPRPFAQIKKNPL"
     gene            complement(36453..38336)
                     /gene="mnmG"
                     /locus_tag="PK460_00490"
     CDS             complement(36453..38336)
                     /gene="mnmG"
                     /locus_tag="PK460_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOE53614.1"
                     /translation="MKFDYDIIVVGGGHAGVEAALASAHMGKNTLLLTMDIKKIANMP
                     CNPNIGGSAKGIVVREVDALGGMMGIFADHDPLQIKILNTGKGPGVRCLRAQEDKVGY
                     PRYVQSIVLNTPNLTVKEAMVVSVIHDTEKVYGVRLENDEEILAEATILTTGTYMEST
                     ILVGHTVKEIGPEGDRSSKGLSPYLEKMGLKIFRLKTGTPARLKKDTIDYSVMVEEPG
                     MEGYLNFSYMSERYHTLEEQVLCHLLYTTPETHAIINEHLGDSAMYSGLVTGTGARYC
                     PSIEDKIVRFSDKERHQLFIEPESRYTDSMYLQGLSTSMPVEVQEKMIRSLKGLENVE
                     ILKYGYAIEYDAIDPLELKPTLELKKWDNLYCAGQIIGTSGYEEAAGLGLLAGINAVL
                     KIDNKPPLILKRDEAYIGVMIDDLVTKGTEEPYRLLSSRSEYRLLLRHDNADSRLTEI
                     GRQVGLVKDDRYAKFLKSQKAIADARAILETTQVSADIPVVEFLESVGYPNYSGGLTI
                     AELIKRPRVTLDDLLELLGHLTLEGLNHNDLFQLETDIKYEGYINKQRQEAKRLKKLE
                     EVEIPASFTYDDLDGLRIEARQRLAKVKPLTIGQAKRISGVNPNDIIILMTYLRRYGK
                     NEK"
     gene            complement(38336..39736)
                     /gene="sufB"
                     /locus_tag="PK460_00495"
     CDS             complement(38336..39736)
                     /gene="sufB"
                     /locus_tag="PK460_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOE53615.1"
                     /translation="MTSADELKFGTYAYGFSDDQESIASTGIGINEDVVRQISAFKEE
                     PEWMLEFRLKSLRAFELMKNPKFGPSLKDLDFDSYTYFNRVVKDEARSWDDVPDTIKE
                     TFEKLGIPEAERKFLAGVATQYDSEVVYASMISEFKEKGVIFSSTDEALKEHPEIFKK
                     YFNTVVKYTDNKYTALNGAVWSGGSFIYVPKGVELDKPLQSYFRLNAAKMGQFERTLI
                     IVDEGASVHYVEGCTAPVYSEDSLHAAVVEIIVHKDAKCRYTTIQNWSNNIINLVTKR
                     ADVYENGQMEWIDGNIGSSINMKYPACILRGEGARGTTISIAVAGKGQYQDAGARMIH
                     LAPNTSSTIISKSVVHSGGTANYRGLVRHTKNAPNSQTHIECDTLILDGISKSDTIPT
                     NEIHNNESFIEHEATVSKISEEQLFYLMSRGLSEEDATQMIIMGFIEPFSRELPMEYA
                     VELNRLIKLDMEGSLG"
     gene            complement(39729..40184)
                     /locus_tag="PK460_00500"
     CDS             complement(39729..40184)
                     /locus_tag="PK460_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOE53616.1"
                     /translation="MPIFNNELMRATILTHYENPYHKETPQNADEFMQIHLTSSGCID
                     NLTLYLKVKDNKVITGYFDGFGCTISIASTSILLEMIEGMNVDAVLKIIAEYKKMLNS
                     EPYDIDILNEAIVFANTVRQPSRIRCATIGWDGVAELITKHKEEENHND"
     gene            complement(40171..41385)
                     /locus_tag="PK460_00505"
     CDS             complement(40171..41385)
                     /locus_tag="PK460_00505"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOE53617.1"
                     /translation="MYDVYKIRKDFPVFDNNKTIYLDNAATTYKPQVVIDAVTNYLSK
                     ETANSGRGDYQNAYLVDKRVDETRALIHKFINSKHVQEVVFTQGTTASLNMVAKCYGE
                     KFLKEGDEILLSIAEHASNTLPWMQVAKKTGAKIVYMPLSEDGKVTPEIVEKYMTEHT
                     KVVSVLHVSNVLGHVHGIKQIAKIVHDKGAIIVLDGAQSVPHMKVDVQDLDVDFLAFS
                     GHKMLAPTGIGVLYGKKELLEKMDPYQTGGGMNFSYDKEGNYELFKVPLKFEAGTLNI
                     EAIYGLHAAIEYINKIGIENISKHEHKLRTYAVSKLKEMDHIILYNENAETGIITFNV
                     KNVFAQDVATYFNSKNIAVRSGEHCAKLLKEHLREVATVRASLYLYTTKEEIDAFVEA
                     ARTAENYLDAYF"
     gene            complement(41385..42218)
                     /locus_tag="PK460_00510"
     CDS             complement(41385..42218)
                     /locus_tag="PK460_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOE53618.1"
                     /translation="MSVFKALLINKMNINDDTLILSENDTVYHIFDENDLPTSVTKVI
                     LKSSKDIDFVVVLRQNKKFITYELAPYTRGKVIFMCSEENLSIDREIILLEGAEVKLI
                     MPDFHNGNRKVNIETKLSERKARAEWHLATYSQNIDKKVFNISFSHFGNESFADMHNY
                     GVVLNASTLIFTGESTIFENVKGAETHQTARIIVFDENSHAEANPILNIYHNDVVAAS
                     HAATVGQVNAEHLYYLKSRGLTEKEAKLLITKGYLMPILNYIEDKVLKENCITMLERV
                     L"
     gene            complement(42215..43027)
                     /gene="sufC"
                     /locus_tag="PK460_00515"
     CDS             complement(42215..43027)
                     /gene="sufC"
                     /locus_tag="PK460_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOE53619.1"
                     /translation="MVLIEIKDLHASVNDIEILRGVNLKINRGEVVALLGPNGHGKST
                     LLQVIMGHPNYKVTKGEILYKGQDVLAMSVDERARAGLFLAMQNPSEVPGVQISDFLR
                     AAINTKREKPIKPIELFRVIDKASKEMQMPHDLASRNLNEGFSGGEKKRHEILQMKLL
                     KPEFAMLDEIDSGLDVDGIRIVSQQINEEKKDKTFLVISHYARLYELINPTRAIVMIN
                     GRVAIEGGMDVIKRIDQEGYEWIQVEHNINIEREDKRPIVLEICAVNERSKQ"
     gene            43355..45481
                     /locus_tag="PK460_00520"
     CDS             43355..45481
                     /locus_tag="PK460_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOE53620.1"
                     /translation="MKTTLIIPNIGCPNCAMAITEHFKTLNIEAKVNVNQKKVTFTYS
                     DKDSFTIILKELKAIGFPAAPIDYDEKRRRKYEKVRLIVATILVLPLAYTMFDNFGLN
                     IVPDIMLNGYFQMGFAAILQFIFGFRFYKNAFYQIKNKNLGMDVLIVLGTSVAFFYSL
                     YLTIFTDKTQLFFETSGMLIWMVTIGDYLEHLSQAKTTDTLKSLMALAVDEVRLIKNG
                     EEQIVSLNDVNVGDVIKVLAGEKIALDGEIIDGASYIDDSVITGESIPKYLSVGDTAV
                     GSTTNLSNTILIKVSAIGSDTVLAKIIKTVEETSLIKPKFQKAVDVIAKYFVFIILMI
                     ALVSFLVYQFILGKELSISLEVTTAVLVVSCPCALGLATPTSIAVASGLAFKNKILYK
                     GGEFFELANKIDAIAFDKTGTLTKGDLIVSDYLGDIRYLSYTKGLEIHSTHPIGKSIN
                     NYRKDIIPFAIEEYEIIDGKGIKGTYLQDQIIVGSATFIAEANISNPFLNEFEKFTGE
                     GKVVIFTAINKKVVNMIILEDEIKAESQTLITALKKMNINVYLITGDNENTAKYLANK
                     VGIENYYSGVLPNEKAHIIKKLQEKHSVVAFVGDGINDAPALKQADVSFSVSNASDIA
                     TDTADVTLLDNDLKLVLIAIDLSKKTTANIKQNIIWAFLYNLVMIPLAIFNIATPLVG
                     GFLHVISSLLVVFNALRLNLYKHKIK"
     gene            45746..47497
                     /locus_tag="PK460_00525"
     CDS             45746..47497
                     /locus_tag="PK460_00525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE53621.1"
                     /translation="MRKPKRIIAGLALSALLLTGCSKDLVADKKGNIVTVTIDGNPVQ
                     INAGDMFEDYLKSSDGLKDYYDAVYEVVVRALFNKEEQTTERTEIYDIAALSVNDVKE
                     QAKNNAKGGGKYDDELKKILKTKNVETLKELEEKLAYDEMQVRLKKQFFDGGEGAWIT
                     NAMEELIVGERNQAGDLILDENNNPVYEGYLNNKLPYHVRHLLVKVGAANNDFVTGKI
                     SKDDANKLSSVIVALANHQDTFGLLANRYSEDEDSARQFGDLGIMNFTTNFVNEFKLG
                     VYAYDALYNADGVMTPEAKAAKLKIPGTAKTKLDALGLDEIPYGAALKLAAVREIEKS
                     DAGKEVNDGDAAFFPRNVIFNKYFNKHNIGVITPNEVNVLDEVGVEKAEFAALPGFKA
                     LPELGGKKVLTDEKGNVILVVRAGSAGSYEGIHFIVVERSALYDTVNGVTLKDYYTIE
                     TPGTAAFPKDDAGNDIDAFVNFLELDARGTKERIDKIRNEVKAFDPTINDRIFQKLVT
                     YLDVKFHDAELKAALDKYLANQYARSQFQLDQDLDKLWSEWIEYLDQQEVARTDRLVP
                     ETYIDDFKAGNDYNYGG"
     gene            47502..49064
                     /locus_tag="PK460_00530"
     CDS             47502..49064
                     /locus_tag="PK460_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53622.1"
                     /translation="MKRKYLLGLAVAALLLTGCDKITAEPVDLDKTVLVDELSEVVNN
                     TLENVYKKYHDSANFKQFVLDEVLLTIAEHEFGPYDKLAADDPFKLAVDTRAKHYFYD
                     ELLSGSYTYRSIFDEKKYVVERVYGTENSYLVDANGNEVAIGAIDTLPFYKEGLFLPI
                     VNKDNFDDPAFKLVHIDYYKHYMQDRYVDKIYREKLTEKFVKEEQTSTLGRNYARKVE
                     YVAIKNNSNHPTAASALVNAFIDNNILGAEPADFNILANAWRGYEPDFIGNEAALLAA
                     TGLKTAEVNKTLIGDIESDFAKIKANPDLTDKAIEDRFTGSGKYPKEVGKQIEINEVR
                     KQSFVTKDWGIKDGGFSSLPEAIRSRLFNIGVANSVDFLKDDAGVLADAGKWEGDAEN
                     KKVASTFVRNIHDDYYLVPKTYEKGNNRNFLFFENDTFYIVKIVEAVNTAKLAAKDDD
                     LNSYEHFKTEAEIAEIVAHVTEALAKLDATKTNALNFYMKDLKIVFHDEDVYDFFKEK
                     FPDIFDDEKKKK"
     gene            49166..49582
                     /locus_tag="PK460_00535"
     CDS             49166..49582
                     /locus_tag="PK460_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOE53623.1"
                     /translation="MSKDCVFCKIIRREIPGAIVYEDNDVLAFLDISPTAPGHTLVIP
                     KEHHETFLGTPRKLMYNVMDVAQRIGQVQIKTLKAQGVNILINNYPPAGQSVPHFHVH
                     VIPRYGKKDGLKIAMVEHLDGDLNFPAVVEKIKSGL"
     gene            complement(49583..50533)
                     /locus_tag="PK460_00540"
     CDS             complement(49583..50533)
                     /locus_tag="PK460_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE53624.1"
                     /translation="MKIKDLNDGIRVIENYLVTNVTKGVSNNGQMYLTVSLQDNTGQI
                     EARVWDANENDVEVFKQGNFVRVVADVIEYRSNLQLKVMTGEVLNPKEVRLDEFTMAS
                     PVKLETLTAELNRLLSSFADTDIQRLVERLISDRYEAFITYPAASRLHHEYTHGLLEH
                     TITIANLADRIIDYYLELYEGDWHINRDLVIAGILLHDLGKTIELSGPVLTSYTVAGR
                     LVGHISLMNAELLKVAEELEIKSEMPILLSHIILSHHGKLEYGSPVTPMTKEAQLVSM
                     IDDLDAKMKLIEKALNTTNSGEFTDRLWALDSTSFYKPKK"
     gene            complement(50523..51293)
                     /locus_tag="PK460_00545"
     CDS             complement(50523..51293)
                     /locus_tag="PK460_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOE53625.1"
                     /translation="MVKIINITLKKEQYNITFDNGEELLVSAETIAEFFLYNGKELSL
                     KDLAKIKEYEAQTKHFSYASNLLARRPYTSHEIREKLRKRCVNEEVIDLIVDKLKRLN
                     LINDLRYALDKIQYLINVKKCSTRAIEQDLLRRGIHPYVIKDALAQSPRDERRELEIL
                     IPKLLKSYRNESLRRAYQKLKTKLYSLGFESDNIDEVLAKFNVEDYIDETENIKKALV
                     KLEKKAKGDTKVLYNKLVIAGYPASLIRKLLEDYSYEN"
     gene            complement(51293..52462)
                     /locus_tag="PK460_00550"
     CDS             complement(51293..52462)
                     /locus_tag="PK460_00550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE53626.1"
                     /translation="MRIAMFTDTFVPDTNGVAVSSYNLFKALNDRGHYCVVVTSNPFS
                     KEVTFIDNVLRIPGIEMKKLYGYNFAGPNRKAHKILKKLKIDIVHVQNEAGMGIFGKS
                     FARQQKIPYVYTYHTMYEDYTYYATKGRMETFAKAIIRRFSRWQAEIADEFIVPSDKT
                     KVSLREYGVQRYINVIPSGVDFSKFMYNPEDKPFLDEYKKEHGLDGTFNLVYVGRVAK
                     EKSIDVVIRGYALYVKKNLNHNTRLIIVGGGPAIPELEELAEKLQIAPFVHFVGKVLP
                     ADIGKYYHLGEVFVSASISETQGLTFMEAMAAERLLLCRFDEGLVGVIRDGESGFFFR
                     SEEDFGEMVKKIRSLSTKEIRNILDEANNIVDYYSNDRFVERCEHVYLRALRNSW"
     gene            complement(52462..53751)
                     /locus_tag="PK460_00555"
     CDS             complement(52462..53751)
                     /locus_tag="PK460_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOE53627.1"
                     /translation="MSEEKVSTTQDLKVQKQTKRDRRYLINIIVILLITFGYTTYSLW
                     NELPAVINVFRGTEADYRMIIIIIVLIALKFFIEGIILFIFARLYTPTYKIHRGVANA
                     FIGQFYNDITPSATGGQFAQVKTFAQQGVPVSIGASIMVMHFILYQIVLVVFGGLLIL
                     LNLGEFARLGSVSIFKLNIPVWVFASFGFLANLVTIVGLFLLAYSRRVHHFVINVVVD
                     LLAKIKIVKRPEYRKNKLYTSTENFRIELRRLSSNVPASILIMVLFFLKFIVNYSVTY
                     FVALMLNPALYGGLSFWETISKTGFLGMITGFIPIPGAAGFAEYFYEIIFSPVFGGDA
                     AFTKAVQLIWRVATFYVNLIVGGFVTAFYRSSMEEIVDETGHVKTFEEVKSYTFAERR
                     ETSVTMYATSQLSIKEIQRRLSPKKKRRWRNHKEDQE"
     gene            complement(53823..56228)
                     /locus_tag="PK460_00560"
     CDS             complement(53823..56228)
                     /locus_tag="PK460_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOE53628.1"
                     /translation="MVNEELKTLLHTLSLTTKLKLLTGANFWESEKVLGTDLPIIVMN
                     DGPFGVRKPTVNVDSGMLMEVIPATAFPTTSALASSFDPELLEKMGKTLATECIDQGV
                     DLLLGPGINIKRSPLCGRNFEYFSEDPYLTSEMASGLVRGLQSKGVGATLKHYAVNNQ
                     EKERYTTNAIVDKRALNEIYLRPFHNVIKNTEPFSVMCSYNQINGRHASRHYKLLTEK
                     LRNEANFKGLVVSDWGAVVDHVDSINAGLNLEMPGGYQNTAELEKAVKDGEIKLETID
                     EMITPLLKFMMYKRDNPKPKVKCDYKKNLEVAQEIAEKSAVLLRNEDNLLPINKTAKV
                     GLIGNFAIKPRYQGSGSSQINPHTLVSVHDAFIQNDVNFVYADGYAEHEVKPNKKKIA
                     EAVKVAKNSDVVVLCVGLPESYEVEGYDRKTLDMPPSHNELIKEVAKVNENIVIVLST
                     GSPVTMPWINDVKSILLTHLAGSMTGPATYNLLFGVTNPSGKLSETYPLSLDKGLPGG
                     EFLTSDQNVLYRESLYVGYRYYDTFREQVLFPFGHGLSYTQFNTSHHEIEEVKDGYNI
                     TMEVTNIGHIRGAEVIGIYTGKSRSLLYGPRKELRAFKKVYLDPGEKQKINIFLPTES
                     LKAYCPVKQEWVLENVNYNVYVGINVQTTDEIFQILVDDANDLSYLKNYQKENEMYYR
                     RADFNPSIKDFTTLFGGESLPLFFANRKRPFTLANNISDTTDYWWGRTIYKIMAKTSR
                     KLAGDDVVLQEMAVETIKELPFRSLSALSSGEVSTNQRDAILLLVNGKPIRGIFKFLR
                     KKK"
     gene            complement(56231..57253)
                     /locus_tag="PK460_00565"
     CDS             complement(56231..57253)
                     /locus_tag="PK460_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE53629.1"
                     /translation="MEAWQIILIVIGAYFLVLFLYSLLIYIFTSVRSKMRCDKNPLFT
                     YYTYEDYQGLKAETFNFKNFKGDNLMARMYTRDGFKDDTYLVFFHGIGAGHEAYTTLI
                     NDLVTTLEMPLLTFDYTGCDFSEGKSIVSVYQPLVDAHAFLAHLQTMPQYKGKKLVLI
                     GHSWGGFVAANLYPFNKDKNIIKVVNLNGVTDFGLYIKHSAKAPFIFVPLFKLFNAFR
                     YGKFAFAATRNSIKNTQIPHLIVHGEKDSVVDFNLFIGNLVLQEDKHKNIKFHFESDK
                     NHSAYLTTDGEKALGEVMGYFLTYSKQKNKDETLKQKIVDFDFSRAVENDPKTLEVIR
                     RFVKGD"
BASE COUNT        17993 a        11200 c         9070 g        19064 t
ORIGIN      
        1 tctcctttat attttacatc gtttggggtg cactaacacc taaaagtgtt aaagcatctt
       61 ttaatacaat ttgtgcggct attgataagc ctaaccgtgc actagtaagt tgcacattac
      121 tttcatctaa aatacgaact tcagtgtaga aagagtgcac taaactcgct agtttttgaa
      181 tatagttagc gattttatat ggctctctag tcattgtagc atcactaaca gcacttgggt
      241 aagcaattaa ttgcttaatt aggttaagtt cagcagcttc ttttaaacct tccccacttg
      301 ggtctagact aaacttttca ccgcgcgcta agacggcgct gagtctagcg tgtgcatatt
      361 gagcgtaata aactggattt gcactagaac tttcagttgc taaatctagg tcaaagtcaa
      421 agtgggtgga cgctgattta gcaaggaaaa agtaccgcgc tgcatcaacc ccaacttctt
      481 cgcacaattc ttctaaagta atcgcattac ctgtccgttt agacatcttt aactcttttc
      541 cgtcttgaat aatccgcact agttgaatga tttgcacttc aagtgtatca gcgggatagt
      601 taaacatttg taacgctgct ttcatgcgac caatataacc gtgatggtca gcacctaaga
      661 catcaattaa aagtgatgag ccacgactca acttatttaa atgataagca atgtcgggta
      721 agaaataagt aaagtcacca ttgcttttaa taatgacacg atctttatca tcaccaaagt
      781 cacttgtctt taagaatgtt gccccgtctt cttcataagt aaaaggcgct aatttttcta
      841 gtaactttgg aatcgtattt cctgctctta cttttgtttc gtagctataa acatcaaaga
      901 caacatcaaa gttttgtaaa tcttttttaa taatatctaa aaatttgttt gtaccttcaa
      961 gcttaaagta ttcaagaccc tctttactct ctttaacata tttatcacca atttcagctt
     1021 ttaaactttc cgcgatggcg attaagtccg tactatgata agcgtctttt ggtaactctt
     1081 tttcaatgcc aaataaattt aagtatcgta cataaagtga tttacctaaa ttatcaattt
     1141 gtacaccagc gtcattaaca taaaattcac ggataacttc gtagccactt ttaataaaga
     1201 gccttgctaa tgtatcacca actgcggctc cgcgcgcatg tcctaaatga agtttaccag
     1261 tcggattagc gctaacaaat tcaatgttaa ctttcgtatc ttttttacta ccattaccaa
     1321 aattactgcc ttcttttaaa atagtcgcaa tatattttga taatgtttct ggttttaaaa
     1381 agaaattaat aaaaccagga ccagcaattt caattttttc aaatggcgtt tgatccatcg
     1441 cattaatgag taaagtcgct acttcacgcg gattcttgcg taacggacgc gctagttgca
     1501 tcgcaatatt tgtagcgtaa tccccatgag cactatcttt actcgtttca atcacaattg
     1561 tgtcttggtc tatatctagt cccagtttca cagttgcatc tttaattaat tcttgtaatc
     1621 ggatatcaat catttcttac ctcaacgatt aagtatactt tatttaaagt atattataaa
     1681 gtaaatccac ttttcacaag caaaaaaggt gattattgct ataaaacaaa caacaaccac
     1741 ctttctttta taaataataa ctagagtcta ttattctgcc cacactgtag gcactccgtc
     1801 aacataacgc cagtgactac catcgtaatt agcggtttca ctataccaat aaacaggacg
     1861 attaccatca ttccaatcag cgtgccatcc atctggtagt gctgttgctt cggcataaat
     1921 tgttagattc gcacaactgc taaaggcaga acgagcaata tttgttacac ttctaggaat
     1981 gacaatttgc ggtaaaatgg tacaacgacc aaaacaccaa ctaccaattt tctccaaatt
     2041 agcgggcagt ttaatctctg gtaaaacttt acaattataa aaggcactgc caccaatttc
     2101 agtcactgtt tccggaatat taacactcgt taaggaagta caatgtgaaa atgtaccagc
     2161 atttatcatc gtaagttcac cttcaattac gactgtttct agctttccac agtcactaaa
     2221 tgcccaatta ccgattttag tgatacttgc gggaatcgta atactttgaa tatcaatgcc
     2281 tgaaaatact tctgctccta aagcttcaat actttcgaca atcaccgtat ctttaacggc
     2341 gaccattaat gtatttgtgg ccgtttcgat aataccatta caattttcgc gtgagtcgta
     2401 atgattattt tcacttacaa catttaatgt tgttagcgcc gtacaatcag caaaagcgcc
     2461 gctagaaata tagcgtacat ttttaggaat cgtaatactt gttaaactct cacaccctct
     2521 aaatgaccca ctgccaaaca ctcgcaaact ttcagatgct ggaagggtag ttaaggcaac
     2581 gcaacttgaa aaagcaacgt caccaataaa cgctaaatta gcgctgtaag taacattagc
     2641 aagcatttca caattactaa atgcccactc ttttatcatt gtgactgagt cgggtatcgt
     2701 aagtgtttct agtattttac aaccactaaa agtccagtcc tcaatgactt ttaagttctt
     2761 acttaaggtt actgaagtta ggctattact atattggaac actgattttc cgattttagt
     2821 gacactatca ggaatgacaa tcgctgttaa attagtttga taaaaagcat aatcttcaat
     2881 tgtcgtgaca ctacttggaa tgactaaagg ctcagtaaat ttccggtaat aaaatgcttt
     2941 ttcaccaatg atttttgtgt cttctggaat aaaaccacca tttaggccga atagtaaacg
     3001 atcggtgtcc ttttctaaaa tcgctttatt cgatggcgag taaaaatgcg tgttattagc
     3061 ggcaacacta agagaggtta acgcaataca attctcaaat gctccgctac caataaatga
     3121 caccttctct ttaatttcca cactttcaag actggaagat ccataaaaaa caccaggcac
     3181 tataagatga gtatcagcgt gaacagtaaa tgtcgtcatc gtttttgtta ttacttccat
     3241 taaagcaaaa tacggattag cggtttctcc ggcgtctcca cttcctaaat atcgtccgtt
     3301 ttcgtaatca gtgtaggtaa cgctagtgcc ataaaaaaca cttgaatcta ttcgttctaa
     3361 actattaggc aatgtaacat ccgctaaatc gggacaatta ctaaacgcct cataaccaat
     3421 tgctttaagt cctgtcccaa aatttagact tgttagtttc gtttgtttaa aagcagagtc
     3481 tgcaattgtt tctaaagaat ttggtaatgt gatggctgtt aatttagact gcataaaggc
     3541 accataacct attgtcttaa gattattacc ccatgaagtt acttctgtca tattactttt
     3601 gtaacaggca aactccgcaa tagtccgcgt atcaggatgt aatttaaatg ttgtttgttc
     3661 tttgtcaatg atttctacaa gaagtaaatg ttcgctaagt gtactattcc caagatataa
     3721 gccattttca taaactttat attcaacatt cggagactta ttaaaaacgc ctaacccaaa
     3781 agttttaata ttcgctgaca tccgaacact tgttaatgac ggacaattag cgaatgcttc
     3841 accctaaatt gtctcaacac tatctggtaa agaaattgct tctaaacttg gacaatgatc
     3901 aaatgccata aagccaattt cttgtacgcc attaccaaaa tcaacatgtt ttaaattatc
     3961 acaatcggca aacacatctt caccgatctt ccgtaaatta tttggaagtg tcactgacac
     4021 taaattatca agattgaaaa atgcccattt ttcgagcgat accactggtt tatcattgta
     4081 agtttcagga atgactaccc tactagcact tccgtaatat ccgcataaag tgtaaccttc
     4141 tcctgtatca gcaaagacga aatcacgcgg atccacaact tcttttaaag ctgttttata
     4201 ttttgctaca tatgttgcat cagcaacaac aggtacaggt tcaggatccc agccattaaa
     4261 cgaatatgta tatgtatcat catcaggccg tgttggtgta gcgccatcat acttcggcat
     4321 cgtgccgcgc tccacatttt tatcaacttc cagtatttgg ctattataat ttttccatgt
     4381 aattgtaaaa gttgttgctt cgcttcctga gtcacctgca cttgtgatag tttcaggctc
     4441 cgttgtatca gtgcttgttg taacacttgg actctcgcta cttggttttt taccacatga
     4501 tgttaaagct aaaaacgcta aaataagcca aaaagctatt gtaaattttt cataaaaatc
     4561 ttccccctac gataacttca ttttactttt aaaagaaagt aaagcaaagc tttttttact
     4621 accttattaa cttttacaat ttataaaaat aattagttat ttataatcaa tattatttaa
     4681 aaattcaatt ttttatttat tttcgcacat taaatttgaa aaagacaatg tcgccatcac
     4741 gcattacata gtctttgcct tcactgcgga ctctaccagc ttctttaatt ttctgttcac
     4801 taccatactt ttcaaaatca ttatatgaat aaacttccgc acggataaag ccgcgttcaa
     4861 agtcactgtg aataataccg gcacactttt gcgcactagt gccatcttta aatgtccacg
     4921 ctctaacttc atctttgccg gtcgtgaaaa aagaagaaag tcctaatgtt ttatatgcaa
     4981 cattaattac tttatttaac cctgattcat taatcccaag ttctgctaaa tataattctt
     5041 gatcagcact tgataaagtc gcaatttctg cttcaatttc agcactgatt gccacaagct
     5101 ctgcgccttc acgcttagca attgctttta ctttttgata ctcaacatta tcctcataag
     5161 ttgcgtattc cgtatcacta atattagcga cataaataag cggtttaatc gttaataaac
     5221 caaagtggag cttaacataa tctgtttctg cttgcgttaa agtagtaact agtcgcgctg
     5281 gcacttcgtt ttctaaggcg gggataagtt tattaataat ctccagttca aacactgaat
     5341 ctttatcttt atttacccgc gcttttgttt caactttatc tttcctacga tataaaactt
     5401 ctaagtcttt aagaattaat tctaaattaa taatctcaat atcgcgttta gcgtcaacac
     5461 tttgttcgac atgaattatt tcttcgccac taaagcagcg caccacctct aaaataacat
     5521 cacattccct aatatgtcca agaaattggt taccaagacc ttcaccacga ttagcgcccc
     5581 gcactaaacc agcaatatcg ttaaattcaa tcgttgcata tattgttttt tcgggttcat
     5641 aatactccgc tagttttgtg acacgcgggt caagtacacg gacagtacct ttatttggat
     5701 taatcgtcgc aaaaggataa ttagcggcct caactttact agcggttaaa gcattaaata
     5761 atgtcgattt gccgacatta ggtaagccaa taattccaac acttaatccc ataatttact
     5821 cccgcagtat tatattacta ttagtcacga taagcaatag tgacaataaa aggctgctca
     5881 tgagaacagc ctcttattta ctcatttaag ttaaattata tcttaacaat attagatgcg
     5941 cgtggtccgc gatctgattg ttccaaggta aattcgactt tttcaccagc agtaatggtt
     6001 ttgaagccat ccattacaag tgatgagtag tggaaaaaga catcagtgcc gtcttctgcg
     6061 cgaataaagc cgtaacctct ttcctgattg aattgtttaa tcgtcccttt catcagtaat
     6121 ccttccatga catatcgtct atttgtgtct aagcacaatt gtattataac aaaaagaaaa
     6181 tttttcgttg cttaaaatag gaaataaaaa gtaagaaagt cgtaaagtta taaatcacgt
     6241 ttcgaaaacg ctaaaaaatc gagtttttca tgctgaaatt taaaataagt tagtaagaaa
     6301 attagtaatc ctgttgcaaa cgctaagaca agtaaatatg gtttcgggtt aaacacatat
     6361 ctgaaactag tttctaaata tttcatcaac actacttcaa cgacaaaaga taaaataaat
     6421 aactgcacaa ttgctaaaag aataataaca cctaggaagc gcaggatata tctactaatc
     6481 accccatgaa ttgcttcttc gttataaccc actaaaaaga gcccgctaaa ttggtcgctt
     6541 gtttcagcaa ctaataagta aattgtcata aaagtaataa gggccgctaa aagaaaagcg
     6601 ccaactgatg ctaaaaaagt ataacgctct aactcgttta atgtatcggc aacaagcact
     6661 gaaaaatctt tatagggata agagacactg aaatgttttt gtaaattttt caagtcttca
     6721 ttagtgacac tctgataaat agcgcccgtt atctctagag caaacgggaa aacattgaat
     6781 acatctttat aaagtaaata tgcccataat gggtgatggt atattacaac ttcatcatta
     6841 tcgattaccc ttttaatcga cacttttatt gtctcgtatt cttttaaata accttcactt
     6901 gcttctgcaa tcgctacaag tgcgccaata tgtaattccg tattatgtgc gtccttgctt
     6961 aaccctaatt tatttgctaa accagaagaa ataattagtt catcaattcg taatggtgta
     7021 ttagcgttcc ggtaattaaa actagaaaac ccattatatt gaatggcaaa atttaaaata
     7081 ccctcaggta attctaagta tgaactaggt gcactatgat cattaatatc tgcccacatt
     7141 gccgcttcta ataaagtaat atcctgcgta ataaaaaagt tatgagcaaa gccaagcatc
     7201 atcatttctg gaataaaagt taaaccatta ctaaatgtat aaaattcagc ttcactctct
     7261 gaagaataac ttttgaacat actattaaaa tgcggaggac tttcaaaaat aagtaaccta
     7321 ttttgtaaat aagttttgtt gtaaatatat tgaaaatagt cgttatttgc cgtgcaaaac
     7381 acataatttg agtaatttaa ggattgccac aagtaatcaa aagcattatt aagtaacata
     7441 taatttatct ttttaagtgt ccacggatat tcttcgattt tcgttaaatt taatgaactt
     7501 gggaacatca ttttttcttc aaaaataaat ggaataaaac taggctcacc cataataatt
     7561 tgtgcttctc tgccaagtgt aactgttcgt aatctaaata tttgttcatc aaaataatcc
     7621 cagttattat tagtcacttc aagcgttaca taaaagttat tattctctag atacactcct
     7681 aattcacttg gggtttgacg catcggtatt cgtaatacat tttggattaa tttaaagtca
     7741 tcaaatggta acactaatcc aatttcatca attgctaaat ttgttacata tggatagagt
     7801 tgctcatgta tctcgtttat atacaaactt tcatttaata agccaatatg aaaagaagga
     7861 aggacatggc gaatgcctag gtgattaaac gacaactcat tttgatgtgg gaaaaaatca
     7921 ttaaaattat ttagataaaa agagcctaat tctttttccg cggagatgtt taacgctatt
     7981 tcttcgtgtg acacactttt aagcacatta atttctggtg cgaacttctt ttgtacatac
     8041 accgtttcat ttgtaaattc ccctttaaaa gtatttacta gtttataatt aatcgcattt
     8101 actaacataa atgataagcc gagtcccact aatcctaagt taacagcacc accagtaaaa
     8161 aagtgtcgtg ctttcttcgc ttttaataat cccctagcta tgtaatcact atttttagca
     8221 tatggtattt ccggcaatgc ttttgcgact ctaagttcat tatttcttat tatatttaca
     8281 cttttaaatt caccatactc cattgtgaaa atggtatcag cgtactcatt agctaaactt
     8341 tcatcatgcg ttactaaaat gatgatgttt gtttttgagt attccctaat taaatccatt
     8401 actattacag cattttctcg atcaagcgca gcggttggtt catcaaaaat ataaagcggg
     8461 acttctttta agagtgtacg agcaatggcg cccctagttt tttcaccacc tgacagtgtt
     8521 tttacttttt gattaagtac tttttcaata tttaaacgct tcgctaattt ttcgacttta
     8581 gagtttacta aattctccag cgacttagtg tgattaagcg ctacaagact aatgttttgt
     8641 cgcattgtta attcttcgat aaaaacactg tgctgaaaaa aagcgcttaa atagcgcgac
     8701 ctaaaagttg tcgtttcctt tttactagcg tgacgataag tgcggttaaa gagctcaaca
     8761 cttccagtat attcactatt taaaccggta ataatattta aaagcgttgt ttttccgcaa
     8821 cctgatggac caagaatcac atataacccc ggagcattaa tctcaaaact agcgatatta
     8881 agaatccgct ctccaccgag tacacattct aaatttttaa tactaatcat ctattaataa
     8941 atacaatagt gggcgacgca tatttatcaa gaaaataatt gtgattaaaa taagtagtgt
     9001 tagaagatca gcgctaagca tcaacactaa catcgtgtaa tttagttcaa gcacaacata
     9061 aacacccatg agtttatgta aaatgaaact tacaacgggt agtaaagaag gtaaaagcaa
     9121 gaaacttacc gagcttaata aaaaattaat aataatcaga aataaatata tttcgtatat
     9181 ttgcttttta ctagcgccta aaacggcaaa aagcgccatt tgtcgtaata tcgttaaaag
     9241 tgcagtatgc gcaatcacca cataaatgac aacactccca ataataacaa aggctaaaaa
     9301 gagtttcatc agtatactta aataatcata caatgcatta aaactctcaa ttttgttaat
     9361 gtgatcacca cttacttcaa tcccgctttc attattgcta acttcattaa caatatgcat
     9421 aaatttatcc ttttgcgctg cacttttaaa gtgacattta aagcggtagt tagatagttc
     9481 atgttctgga tttaaattaa gcaaataatt attaagtgtt aaaccaggct ttaaaaatat
     9541 attacctaag gttgcatcaa tcgtattttg ggaaatgtat aatttaggga cagaaaaata
     9601 tgtcggttca tcgtatatat aagtaatagg aaactgatta taataatcaa ttaacacttc
     9661 ttcatcatcg ataaatattg tcgtctttat ataaaaatgt agcaacattt catcatcctt
     9721 tattgataca agtttattaa gttcgttgta aaaggctcgg ttaatgccaa tctcattagt
     9781 gttctcccca ataattgtaa ttaagacatt aggaatttct ttacctaaaa ccataatttc
     9841 tttttcataa agtaagggtg aataatctgg tctaataaca aaagattctg ttactttcat
     9901 tatcgaatta aggtaatcaa tacttggtcg tcgataagat ttaacattaa tcagcggatt
     9961 gttagtaggt tgcgattcat aaagggcaac atccacactt aaatattcta aataaccttc
    10021 cgcaagagtt tgggctagtg tttcactact tgaaaagaaa actaaaaata acatcagcgt
    10081 gatattagta aaaatcataa aaataaccat taatcgattt ttctttatat ttcgtttata
    10141 taaattagca ataactttaa ataatgttat tatcttcatg aaaaactccg ttcttaatcc
    10201 ttaaaaaaat agtattacca cttttaaata aacttggttg gtgtgttgtt actattacga
    10261 ttttattagc gcttaattcg ctaagaagct tcgctactat ttgactattt ttaaaatcaa
    10321 gatgggctgt tggctcatca attaataaaa tgtgttttcg ttcaataata ccacgcaata
    10381 tatttgcacg cgccttttct ccgccactac atacatttgc tcttctttgc aaaatatggc
    10441 tgatttgcag tgctttagtg tatttttgtc gtatttttcg attgtttaaa tggcgaaaaa
    10501 gtgaactaat tttaagatta tcaattatcc ttaaatcacc taaaaaatat ggatgctgca
    10561 aaactaaagt aacatcattt ttagtaatca gtttattttt atatttaatt ttcccactgt
    10621 ttttagaaat ataattagcg acaatatgta aaaaggttga ttttccgctt cctgaataac
    10681 caacgatgac atatatcttc ccacttttaa aagtataaga aagattacta aatatttctt
    10741 ttttgccaaa acttttatat agattaacga cttgtaaaat aggaacctcc tactttacaa
    10801 ataaaacatt atttcgtaaa aattatcgtt tcgtgactag caatgtgttt tttaaacttt
    10861 tgattaaact taatcctgct aattaaaacg atgtttccac aaccctgaca ttgcaatttt
    10921 aaatcagcac caagcatcac cacttgaaag cgatgactct tatgaatgca aggatgcggc
    10981 ttcttcattt caatttcatc ataaagatca actttaatta aagccactag cgaacctcct
    11041 tcatattaac cgcagcaggt actttaggga gtccaggcat tgttaatact ttaccagtta
    11101 gggcaacgat gaaacctgcc ccagcactta gtctaaattc gcggattgta atattgaagt
    11161 ttcgtggcgc attttttaaa tttggattat cactaaatga taacggagtt ttagccatac
    11221 acaccggtaa tttagcaaac ccttgttctt taattaccgc aagttcgcgt tgtgctcttt
    11281 tactatattt aaccccgctt gcgcgatata actttttaca tataatttca atcttttctt
    11341 caacttcttt ctcagtatca taaagaaatt cgaacttagt ttcttccgct agtaatgctt
    11401 ttactttatt tgcaaattca ataccacctt cactgccttt actcaccgag ctaacaagtg
    11461 cataatcata tcctagtttt tgcatttctt tttcaaataa cttcacttca cttttatgat
    11521 cattatcaaa aagattaaga gcaacaagaa ctttaacacc aaataaactt aagttttcat
    11581 aatgctgcac taagttttca aatcctttgg caagtgcatc agtattttct tcataaattg
    11641 cactcattgg aacgccacca tgcatcttta atgccttaat agtggcaact acgaccgcta
    11701 gattaggctt taaattagcg gtacgacact taatattgaa aaacttttcc gcacctaatt
    11761 cactagcaaa acctgcttca gtgataacga ctggtgcttt acttaacgca tattttgtcg
    11821 caatgattga attacagcca tgggcaatat ttgcaaaagg tccgccatgg acaagaacgg
    11881 gattgccttc taatgtttgc accaaatttg gtaataatgc ttgtttcatt aagtgcataa
    11941 tcgcatcttc tatttctaaa gcggcaagac gaacaatttc accttcatat gtatagccaa
    12001 taataatatc ttttacacga cgaataaagt catcaggatc actagcaaga cacataatgg
    12061 ccattagttc actagcgaca gtaatttgaa attcttcgca gcgaggagta ccattgcccg
    12121 taccttgatt aattctaact ttgcgtaagc cacgatcatt catatctaaa gagcgtttcc
    12181 atgtgatacg tttcgggtct aaatttaaag cattgccttg gaaaatatga ttatcaatta
    12241 ctgcggcaat tagattaatg cttgaagtaa gagcgtggag atcacctgtg aaatgaagat
    12301 taatatcgtc acttggataa agcttagcgc gtcctgcacc agtggcacca cctttaatcc
    12361 caaacactgg accaagtgaa ggctcccgta aacaaagtaa actctttgtt cccgttttcc
    12421 acattgcgtc ttgtagagca attgacattg tcgttttccc ttcaccaaaa ggtgttggtg
    12481 taatcgcagt tactaaaatt aatttacctt gtggattaat atcagtaaac ggacttttaa
    12541 tctttgcttt atcttcacca taaagtatta catctgcagg tgaaagacca actttctttg
    12601 caacttctaa aatgtgtaat gtgtttttca taatgagtcc tcatactttc ctcttagata
    12661 attaaacgct gcttctttat cagtaaacca tttagcggca aactgattac gaaaaaaagt
    12721 tcgctgtctt ttcgcatatt gtcttgtacg taaggcaatt aaatttatta attcttcatc
    12781 agtggcatta ggattttgca caatctcttt ataaccaatt gcctgcattc cctgtgcatt
    12841 tcgcgggtat ttcttgctta atgtcatcgc ttcttctctt aatcccttgt caaacattaa
    12901 cttaactctt gtttcaatat cgcgatggag catttcattt tctttatcta aacaaatcat
    12961 aatatacgga taaagtggct cacttgatgt tacttcttca agcgcagttt ttgtttgagc
    13021 gtgagtttta attaaagcga gtgcccgcag tacgcgctta cgattattag gatgaatctt
    13081 agcggcagct tctttatcta acacttctaa ttctttgtgc agtgaagtat tacttatgtt
    13141 agggtcatca acaatcttta agtccttttc cggttgtaag ttatagttat ataaggctgc
    13201 tctaatataa agaccgctgc caccaacgag cactatcgga atttttttag ctaaatactt
    13261 atctaatata atccgtactt ctttttgata gttataaata gaaaagcgtt catctaatga
    13321 aatattgtta aataaaaaag tcggaagcat cgctaattct tctttggttg gtttagcagt
    13381 tcctatattt aactctttat aaaaagcaaa cgcatcagcg ttaataataa ttccgtttat
    13441 tgctttagca aagcggaagg ctaaatcact tttcccgctt gcggtcacac caccaatgac
    13501 aaaaatcatg tgcctgtcct cttaaacatt ttttctaatt cgtacttcgt aaacttaata
    13561 attgtcggcc gaccatgcgg acaactaaac ggattatcac acgctagtaa ttcttttatt
    13621 aaatgttgca tttccataat tgaaaggcgg tgattagctt taattgatgc tttacaagcc
    13681 attgttgcaa tagcgttagt gcgtaaaagc tcttgattta aacgcttatc agtaataaca
    13741 gcatcaatga tatcttgaat ataaatgcgt tcatcatatt cacctgccca actaggcaca
    13801 gcagtaactc ttagtgtatt tgggccaaat aactctgctt caattccgac atctttaaga
    13861 atggtaaata cttcaggagt aaaaagcgct atttcactat gactaaaaga taaaagttca
    13921 ggcactaaaa gtggtctagt ttttaaatcc atatttaata ctttttgaaa tttctcataa
    13981 ttaatccgtt catgagcagc gtgttggtca ataagccaaa agcccccatc atttacttcg
    14041 caaataatgt aggtgttatg gagtgctgca actggattaa caaaactatt ttctaattca
    14101 ctaattgttt tagttattgg ttttctaatt gcttcttgat gatgactatc actaattttt
    14161 aattcactag tttcagtgcc aaacggcaaa tcaaaaataa gttgctcggc tctaggttct
    14221 tcgttagtag tgcttgatag tggttgcact ttttggtgcg gatctttagg gataaatgct
    14281 tctttacgga gcgctagcgg aatttctttt aataaaacat tgcgtaagtc ttcttctttt
    14341 gaaaaacgca cttctttttt agtcggatgc acattaacat cgactaattc tggttctatt
    14401 tgtaaattta aaatgacaaa cggataacgc gtgggagcaa taaagccgtg atacgcttca
    14461 attatcgtac tttgcacttt cggcatatat acattacgac catttaagtt tgtaatgatc
    14521 ccgtaacgat tagaacgaac taaattcgtg ttccctaaga aacctgtaat cgtaaaatca
    14581 ttcgttttaa cactaattgg taataagttt ttaacatatg ttaaaccata aatatttaaa
    14641 attgtttctt ctaaattatt tcgcccactt gtgcgaaatc ttaacttatc atcaaatgtt
    14701 aaactaaaac taacatcagg atgggctaaa gctattttag tcacaacatc aagtgttaca
    14761 gcgttttcgg tataatcgcg ttttaaatat ttaaggcgcg ctggtgtatt gtaaaaaagt
    14821 tcagagacag taatttttgt accactacta actgcacttg gctcaatttt aatatcacca
    14881 ttttcaatct tagcaagtgc tccaggtttc cccttttcag cagtttcaac ttgcactttt
    14941 gcaattgaag caatacttgg tagtgcttca cctctaaacc ctaaagttct tattctaaat
    15001 aaattgaaag ttgaatgaat cttacttgta gcatgtcgcg aaaaagctag cgctaattcg
    15061 tcgctaggaa tcccgctacc attatcttgt acaattattg ctttacgtcc ggcttcatta
    15121 ataaaaacat caattttagt agcaccagcg tcaatactat tttccactaa ttctttaaca
    15181 actgaacttg gtctttcaat tacctccccc gctgcaattt gattagcaag ttcgttgtct
    15241 agtaaaatga tcttagccat tatttaaacc cttcatcttt aattttcttt aaaagaataa
    15301 gggcatctag gggagtcatt tcatcaatat ttaaattttc gagctgttga taccataatg
    15361 gttttagtgg cacatattct gttttagtcg gattgccgcc actattgtta gcgacaaatg
    15421 tttgtaaatg ttcttgggca ttacttaaaa tttctagcgg taatcccgct aaacgggcaa
    15481 cattaatccc gtaactctta ttcataggac caggtttaat tttataaagg aaggttacat
    15541 tattttcgac ttcttttact tcaacatgaa tgtttttaat attaggataa tgctcatcaa
    15601 ttgcagtaat ttcgtgatag tgcgtgctaa ataatgtctt tacttttaac ttgttagcaa
    15661 tatattcaag cattgcgtag gctaaagcca ttccgtcata agttgaagtg ccgcgaccaa
    15721 tttcatcaaa tagtaaaagt gaatttggtg ttgctttgcg taaagcataa tttgcttcaa
    15781 tcatttcaac cataaatgtt gattgtccgc ttattaaatc atcgcttgcc ccaatccgtg
    15841 taaagatttg atcaaaaatc attaagtttg cgctctttgc cggaacaaaa ctgccaatct
    15901 gcgccataat cacaattagc gctaattgac gcataagtgt cgatttacca cccatatttg
    15961 gaccagtaat aatcacgata tcggtcgctt catctagctc aatatcattt gcaacaaagg
    16021 cgtggtcttt aagtaattta cttaaaacag ggtgccgtgc ttctttaata tcaaccacgc
    16081 gtgcatcgtt aaatatagga cgaacataat tattagcgac cgccacttcc gctagagaaa
    16141 taaaaacatc aatttcactt acaacatcgg cactttgttg taataatcgt gttgacttct
    16201 ttaactcatt taaaagcgca ccgaaaagtt ctctttctaa ttgttgccgt cgttcttcag
    16261 cacttagaat tagtgtttct tgttctttaa gcgcttcatt aataaagcgt tcaccgttct
    16321 ttgttgtttg tttgcggata taaccaaatt catctttaac ttgatctaaa ttgctattac
    16381 taacttcaat gtaataacca aatacacgat tataaccaat tttcaaattt ttaattcctg
    16441 tccgcactcg ttctttttct tcaaaagcac taatccattt tttaccacca tgcgctaaat
    16501 caattagttc atcaagcgct tcataatatc catacttaaa tatttcgcca ccagttattt
    16561 gtagtggggc gtcttcacta attgcttttt ccagtaaggt aattaaagga atattacttg
    16621 caagcttatc atagagttca ctaatttcag gttctctaat tttacctaaa agattacgtg
    16681 ctttttctag tgcttggagt gaagtcttaa ggcacaacaa gtcacgcgga ttagcgctac
    16741 caaaagagat gcgcgccact atacgttcta agtcataaat actaacaaaa tgttctttaa
    16801 cttccgcacg tgttacaaag ttaacaataa atgtgtttac aatattttgc cgccgaataa
    16861 tttcatcata atcgcgtgaa ggacggataa taaaatcttt tagtttacga cgacccatcg
    16921 ctgttgttgt ttcatctaat agccaaaata gagacccgta ttttgtttca ttacgaattg
    16981 ttcttgttaa ctctaaatta aggcgtgaaa aagcatctat ttgtaagaaa gactgcggtt
    17041 ttgtcacagt aattgcttta aaatatgata attgtcgttt tttagttcgt gttaaatagc
    17101 tcactaaacg cgcggcaatc aaatactccc gctcatcctt aacatcacta aataaatctt
    17161 catattcttc atcaattttt gtattctctt caaacgatat aacaagattt atatttgttt
    17221 taataacacc taaaagctta tcactaaact gtggactcac aacaatttca cgagtgttta
    17281 atccgtttaa ataacttact aaactcgtat cttcacgcgg taaagttgtg acgaaaatgt
    17341 caccagtaga aacatctgcg taacttacag taaaagcatc agcgtaagca tcaaccgctg
    17401 tgataaagtt attatcacta tcttcaaaat cgacaatcga accaggagtt acaatttgga
    17461 ttatttctct ttttacaatc ccctttgctg aactaggatc ttccatttgt tcaacaattc
    17521 caaccttatg cccagcgtca attaacttgg caagatacgg accataagcg tgatggggta
    17581 tcccgcacat tgggatttta cgattatttc ctgctgctct tttagtgagc actaattgta
    17641 attctttgga tacaatttca gcatcttcaa aaaagaattc ataaaaatca ccaagtcgat
    17701 acataattaa aatacccggg ttttcccgtt taatttctaa atactgctca atcatcggag
    17761 tatagacgat ttttttcttc tcttttccca tagtgtcatt ttaccatagt atggtaaatt
    17821 gcttattcaa ttactgtttt taaagagtaa agtaaatctt caatttcact tagcaaacta
    17881 ttataattta ttagataagg atgcgtttca taattgtttt ttagtcgtaa atattctctt
    17941 ttatactcgt tatgcttttc ttcgttaaag gcgttttgtg tcattaatcg ttgtaactct
    18001 ttaagccgta cttctacatt ttttaagtaa gcatcacttt caatcagtgc ttttactttt
    18061 ttaaattcta cgactctttt gtcattcgct aattcagtcg ttaatgcatt taaggctatt
    18121 tcaactttat tcattcacta gttctccaac aaatgaataa acgcgtgact cagtaatttt
    18181 aactttaaca attttaccga ttaaatcagg agtacctaca aaatgaacaa cacgatcact
    18241 ttcggtgtat ccacttaata cactcgtatc ttttttactg taaccatcaa ctaaaataag
    18301 atactcattc ccgacatata gcgctgcatt agcttcggtt tggctatcaa cgagtgcttt
    18361 taacttgtta aagcgctcac ctttttcttc acgcgaaaca ttatcttcca taagggcagc
    18421 gggagtaccc ttgcgcggtg aataaataaa agtataagca ttttcgtaat ttaccttttc
    18481 aacaactttt aatgtttcta aaaattgttg ctctgtttca tttggaaagc caacaataat
    18541 atcagtagtc agtgaaatat caggcattct agcccgtaat ttagtaacta actctaaata
    18601 atctttaacc gtatagcggc gtcccattaa ctttaaaatt tcatcactac cactttgtaa
    18661 cggtaaatga attttacgca tgatattagg atatttagcg ataatatcaa tcatttcatc
    18721 atcaaagttc catgggtgcg aagtaataaa acgaaggcgt gggatgccaa gttttgctac
    18781 ttcttctaaa atataactaa aagttgtgcc atcttttaaa tcaataccat aagcattaac
    18841 attttgacca acgagtgtaa tttcttgata accagaatcc actaaaattt gacattctcg
    18901 taaaatatca gcaggtaatc gtgagcgttc tttcccccta gtgtaaggga cgatacaata
    18961 tgtacaaaac ttatcacaac catatgttat attgacaaaa gctttatgcg gggcaatgcg
    19021 ttgcacaggg atgttttcat agacatcggc atcatcacta ggcacatcca ctactttttt
    19081 attttccgtt attcgtaagg caattaaatc aagtaattta tgaatagcgt ttgtccccat
    19141 aaaaatatca acatatggga atgtttttcg taatttcgcg agtgattctt cttgttgcac
    19201 aacacaaccc gcaacaatta ctattaaatc tttattattt gtttttagtc cttttaagtt
    19261 accgacttga ccaaagattt tttgttcagc cccttcacga acagcacatg tattaataat
    19321 aattacacta gcgtcttttt cttcttcagt gcgtcgcata ttagccatac ttaaaagacc
    19381 ggccatactt tcttcgtcgc gtacatttgc ctgacagcca aatgttttaa ttaagaaaaa
    19441 tttaccactt aacttcgcac taacaattga cggtacttca atcttaagat tttgaactat
    19501 ggacggataa cgtcttcgtg ctttagtgaa ggagggtaaa ttattaagta atgttttact
    19561 catctcttct ccataagtaa taaagtggtg ttattttttc tgttttaaat gtatgtttat
    19621 taatttctaa aataacagcg ctaaattgcg cttctttatc tttgtactca taagtaaacg
    19681 gtacttgttt atcaaaccaa gtccgctcga ttacgcgttc tctatctgca ccaatgatgc
    19741 cattatatgc ccccgtcata ccaacatcac taatataagc tgtacctttt ggcaaaatac
    19801 gggcgtcacg cgtttgtaca tgagtgtgtg tacctaaaac agcacttact gcaccatcaa
    19861 attcccagcc aatcgtcatt ttttctgcag tagcttctgc atgataatca acgatatgaa
    19921 tcatcttttc tgtatcagcg ttaagcattt taagattagc gtacggacta gtaatgattt
    19981 ctttcataaa tgcttgtcct aatacattag tgacgcgaac tttgtcacct tttttcgttg
    20041 tgacaacgac agatccttca ccaggataat tttcaattaa gtttaatggt ctcactaaat
    20101 tatgcacttc ggtaatgtaa tatttaagtt ctggacgatc atcaaaatga ttaccaagtg
    20161 tgatgacatc aatcccaaga tttactaatt cttcataatg ttttttcgtt aatccgcgcc
    20221 catgcgttgt attttcgccg ttagcgataa ttaaatcaaa gtcatgttca cgcttaagta
    20281 taggcaggaa atgggcgaga gtttctcgcc cgattttgcc aacaatatca ccaataaata
    20341 aaattttcac cattactatt ttgctacatc acttgcacgg tattcacgaa taaccgacac
    20401 tttaatttga ccaggataag tcatttcatt ttcaatccgt tcacgaatat cacgaacgag
    20461 tttattgacc ataatatcat ccaccttatc aggatggacc atcacgcgta cttcacggcc
    20521 tgattgcatt gcaaagctag tttgcacacc gtcatacgat ttacaaattt cttcaagttt
    20581 ttctaaacgt ttaatgtaat tttcaagcgt ttcagtccga gcacctggtc gcgctgcact
    20641 taatgtatca gcagcgatga ctaattgact aaagataaaa cgcgattctt tatcaccgtg
    20701 gtggctttca attgcatcaa taacataatc tggttcattg aattttttcg ctagtcttgc
    20761 tccaacttca acgtgacttc cttcaagttc aaaatccgct gatttaccaa tgtcatggaa
    20821 taatcctgca cgtttagcgc gcgtaatatc aagccctaat tcagcagcca taatgccaca
    20881 taaatgggcg acttcaatac tatgtttaag tgcactttgt ccgtaactcg tgcggtattt
    20941 taagcggcca ataaatggaa tcatatcttt gtggacatta ggaatcccaa gttcggcaag
    21001 agcgtcttta ccagtcttaa taataatttc atcaagttct tgcgttgctt tttccacaac
    21061 ttcttcaata cgacttggtt gaattcggcc gtctttgact aatagttcaa tcgtccgtct
    21121 agcggtctca cggcgaattg gatcaaagca tgacacagta ataacttctg gtgtatcatc
    21181 aattaaaata tcaacgccta atagttgttc aagtgtgcgg atgttgcgtc cttcacgacc
    21241 gataatgcgt cctttcattt catcatttgg taattcgacc gtactaacag tgcgttcagt
    21301 cgtcacttct tgtgaataac gcgcaatcgt gtttgctaaa atatcacgag cttgttcatt
    21361 aacttgtagt tcggcttctt cttctttttg cttaatatac agcgcgattt ctttttccag
    21421 tttagcgtca acgcgatgat ataattcatc tcgcgcctca ctaactgtca tttgtgcgac
    21481 tgtttcaagc tcgcctaaaa ttgagtcaat cttaatttgt aggtcttttt tgagttcatc
    21541 agcttctttg attttcactg ataagttttc atgacgagcg tcaagtgact gctctttgct
    21601 atgtaataat tgatcgcgac gttcaattcc tttttcacga tcattaagga gcctttctag
    21661 ctcacgaatt tgggcacgcc gttctttgcc ggcttgttcg agttcttgtt ttccttcata
    21721 aatgatttgt ttagcgtcaa gttcagcatt ctttacaatg tgttcggctt taatttccgc
    21781 ttcttttgtt aatttttgtg ctttcgcaaa tgcttgcttc gctttaaatg ctggaatgag
    21841 gaaaaatagt ccgattccaa gtccaagtcc gacaagaaag aatagtgcat agtacaaaac
    21901 ttccattttg tgttccttct ttctttgttt tttaaaattt tgtgtttgaa aatatataca
    21961 aacgaaaaat agtctgttac ctaaattcca atgttacatc attaatgttt agcgatgatt
    22021 tatgtaaata aatttcaata aagtgaatta ataacctaac atgaagttta ggtgaacgca
    22081 cttagtgcgc actttgatta taacaacttt ttagcattaa ttaaagcaaa gatgattagt
    22141 aaagtacaca attttaaaaa ttgtgttttc taattttaaa atttataaaa taattgtaat
    22201 tatttagttt cagtggcgac tacgcctgtt ttaatttttt cttctaaatc agcgagtaat
    22261 tctgggcgct ccaaaatata attttgcgct gcttcgcggc cttgtccaat cttaagtcca
    22321 tcgtgttcat accacgaacc cgacttttta ataaatccgc ggttaaccgc taattcaaga
    22381 atttccccaa ttttagaaat acctttgcca taaattatat cgattccaca agcggcaaat
    22441 ggtggtgcaa ctttgttttt tacaatacga atttttgctt gattaccaat taatttagaa
    22501 ccttgcttaa tcgcttcacc gcgtctaatg tcaatacgaa tggaagaata aaattttaat
    22561 gcacgaccac cagaagtaac ttcaggattg ccataaatta ttccaacctt ttcgcgtagt
    22621 tgattaataa agataacagt acactcagct ttatttaata cacccgctag tttacgcatt
    22681 gcttttgaca tcaggcgtgc ttgtagtcct acttggttat cacccatttc gccagatagt
    22741 tctgcttcag gtacaagtgc agccacacta tcaacaacga ttaaatcaat cacaccactt
    22801 tcggcaagac ggacaacaat ttccagtgct tgttcacctg aatcaggttg tgctaaaagt
    22861 aattcatcaa tattaattcc taaattttta gcataaatcg ggtcaattgc atgttcagca
    22921 tcgacaaaag cagcttgacc acctttcttt tgacattcag caattgcgtg cagcgctaaa
    22981 gtagttttcc cactactttc tggtccaaag atttcaataa tacgcccttt tggatagcca
    23041 ccaacgccaa gtgcttcatc aattaataat gagccgctcg gaatgacatc aacattaata
    23101 actgggcgtt cgcctagttt cataatggcg cctttaccaa acgacttttc aatttctttt
    23161 aatgtttctt gtaaaatatt tgcatctttc ttttttgaca taataatttt ctccttatat
    23221 agatatatac aatattattc gaagtttttt aacactctcg ctaacatttt taaggcagca
    23281 ttcactgcat cagtgcggac tttaagccga tcacctttaa atttgaagtg ttcaacaatt
    23341 tcgtcgttag caaacgctag ggcaatataa acatcaccaa cttctccgct ttggcctgat
    23401 gctgttggac ctgcgtcacc cgtaaagcca aaacaaaaat caactcctaa tgctttttgg
    23461 ccatgacgcg ctaaatcaag cgctacaaca cttgaaacgg cactaaattt ttttagatct
    23521 tcttttttaa tgttaagtaa acgctctttg acatcgttac tataagccac aatcgtccct
    23581 ttatatgttt tactaacacc tggataattc acgagtgttg ccgcaaataa accaccagta
    23641 aaagactcaa cactaccaat tgttaaattt ttatcaatta attcattaat gagcagttgt
    23701 gtcgtcatgc gtctccttaa gtacactgat attttggaat atataaataa agcctgataa
    23761 taatgacaca aatgtagcag cgccaattac tatcatgcta acaatcggaa acgaatcact
    23821 ttgcatttgg tgtgaaaagg gccaatcatt aaataaaaac atcacaattg caatcatttg
    23881 taaaactgtt ttagccttac caaaaatatt cgccgctagt actttatttt gatttgcagc
    23941 aacaagtcta aacccgtcca ctactgtatc acgcgcaatc atgagtacaa caaaaataac
    24001 aggtacactt atttgcccga cgaacgcttg atgcggaact gtaaggatga ttaacataga
    24061 attcaccagc attttgtcgg caattgggtc cataaacttc ccaaaatttg taattaaatt
    24121 aaattttctc gctaagtatc catcgaagta atcagtgatt gccgctacta taaaaacaat
    24181 tgcggcaata aaataacgca gataaacatt agaaaaaaga ataattggtt cattacctaa
    24241 ccaagtgggc ggaataagta aaaaagcaat taaaaggatg acaagtatca ttctcgctac
    24301 tgttaattta ttcggtaaat tcataatgct cctttaggtg agtttcgatc ctgtaagcca
    24361 tgttctgtcg cggataatta tttatctatg tgtgaacaca tacggcgcta aattcatttc
    24421 tctattgccg tttcccttac caaatttagg tttctcgctt gcggggttta ccaacgttcc
    24481 acttaagagt ttccccttaa cttcgtcact gtggcacctt cagaagatta accatatctt
    24541 tcgacttagg tctttcttcg ccgttataga ctcctctata cctagtgtta ttttttccac
    24601 tagcgcaatc actacttgca tcgcagcaag tgcgagcatg gactttcctc taatacttat
    24661 gtaccagcaa ttatccagat cgaattttat ttgagtttgc ttgggtcaat gcccattttc
    24721 cataattgac gctcatactt atcaatttta cgtaagtttt ccatcgcggt tccgccttta
    24781 ctaattaagc gttctagctc ttcaactttc tttttatatt gaatgtattg cgcttcaaga
    24841 ttaacaatct tagcttgttg agtttcattt ttcagccgca gtaattctaa ttctttttca
    24901 atttcgagca tagattcata gtcattacgc actaaatcaa aaaattcgtc aacttctaaa
    24961 gcgtcatagc caccgctttt cgctttgaac ttctttttta aaattttgtc tgcgtctagt
    25021 aatctctttt ctgccatcct taacctcgca tacatattat atatgtaatt ttgttttgac
    25081 gcaacaaagt gagaccgctt tattattact atgttataat tttatagtat gaaaaaatta
    25141 gcgaaagcaa cacgtggccg caagcaactt atcttccttg atttagaagg tacacaattt
    25201 agtcatgaac taatagcaat tggggcaatt aaagccacaa ttaaaacgga cggaacgatt
    25261 aaaaaacttt atcctggctt taaacattat gtgctagcaa cacaaaatgt tggtaattat
    25321 attgaaaaat taactggcat tacggatgat ttattagcaa gagaaggcat tccttttgat
    25381 aagattctac tgatgcttaa aaaatatgtt ggcactaatc tttcaaaaac taaatttgtg
    25441 acatttgggg cacacgacat tcgtatctta cgccaatcac ttgcccatac tcctgaagct
    25501 catcatactt ttattaaaac gattgcgaaa aatcatattg atttaagtgc atacattaat
    25561 gaatatataa aagatgaaaa aaataataca ttatctttgg tgaatttatg taaacttttc
    25621 ggcgttgagc cagttgaacc tgctcatgac ccgttaaatg atgccctgat gctcgcctat
    25681 ttatatagtg agctctttaa aaagactgat attcttattg aacggtatgg tgaggtacta
    25741 ctcaatatga ataatatccc gcgtccaata aaaagaatgc tcaataaaat aaaggcagga
    25801 gaaagtgtaa atattgatga tttacaagaa tatttacgcg atgaaattgt gtaatgatta
    25861 attatccaaa cggttctaaa cctaaaaaac aaacaacgaa agtaccttca aaacgaaaaa
    25921 atattacgaa gagcgctgct aatcgtggca tggcgcttga aaaacttatc agtgctgcta
    25981 atgactatta tttgatgcac gacattgccc tttttactaa acgcccaaca ccaattaatg
    26041 tcgtaaaaat tgattacgct aaaggggcaa aaattacaaa cgcctacttt gaaaagcaat
    26101 caacaacaga ctttaacggg gtttataaaa accgatattt cgactttgaa gctaaaagta
    26161 caagattaaa aacaagtttt cctttaaata atatttatga acatcaaatt acccacttag
    26221 aaaatgttat tcgccatggt gggattgctt tctttattgt tgaatttaca gcttttgatg
    26281 aatattatgt acttagcgct gctgatgtta ttgattttta tcgcaatggc gaccgtaaat
    26341 caatccctta tgcatcattt aaagaaaaag gcgtattagt gcgccttggt cttaaccctg
    26401 tacttgacta tttgcctgca gtcataaaat tatttaattt ataacttttt atttggttct
    26461 ttacaaatgt ccacaagcgg acatttttta catagcggac tttgtgcttt acaaaaatac
    26521 cgaccaaaat gaatcatttg gtgatgcgtt tttatatatc tttctttagg taaaacttta
    26581 cgtaacttcg cttcgatgat agttacatca tcactaaact tagcaaaacc aaggcgacgc
    26641 gctattcgtg aaacatgagt gtccacggca atttcgggaa tgttaaagag ttcagcgcgt
    26701 acaacattcg cggttttaac accaacacca ggtaatttta aaagctcttc tttagcacta
    26761 ggaacaactc cgttatgatg ttcattaagt aaatgaatta aattatacac atgttttgct
    26821 ttgttgcgat ataagccaag tgttttaaaa tacgcggcaa attgtgcttc ttttacattt
    26881 aataagcttt caaaagttgg atactttgca aataattttc gcgtaacatt attaaccgct
    26941 acatcagttg attgtgcgct taaaacaaca gcaataagca gttcataatc ttttgtgtat
    27001 tctagctcac ctttagcgtc actatattct ttatcaagaa aggcgagtat tctttcttta
    27061 ttcgttctcg tcatcttggg cttttttatt cacttcttca agtgtgtcct tgatgttttt
    27121 tcgatatgaa tcactttggg cacttttacc ttcaagcgca taatccacag cagtcgataa
    27181 tttaagtagc gccttatcaa tagcgcgaat gttatagcgt ttacgtttca ttgtttggtc
    27241 aagtgcttct ttaatcattg tgatgttata accataatta aaccaatcat taatcgtttg
    27301 cagttctact gctgaaagtg aacggccaaa agcattttga ataaaggcat aaacttcttc
    27361 aacttgtgaa ctaaattcta ctgtcgaatt ttctttattc ttttcataat ctaacacgaa
    27421 aagttcttgt aatttatttc gtaacggatt aagtgaagta accattttcc cgtttctagt
    27481 cgtaaattca attaaacctt tactaacaag acgaaccatt gcttcgtcaa tcactttgat
    27541 atcaatattt gattgaagat taattaaatc aggagtaata aattctgttt ctttttgttg
    27601 taagtgctca ataaaaagaa tgattacaag ttctgtttca ttgatacgga gctctttgta
    27661 atattcaaaa agcataaagc gaaaatctag cgccttatta tacactacga tctttaaccg
    27721 cctttgttaa attacgtggc ttatcaacat cacgtcctaa gttaactgca caataataag
    27781 caagatactg cgcgaatact gcaaaggtga ttggactatg ataaccctta attgggtttg
    27841 aaataataaa gctatcacca tcatgattaa atggcgcaag tgagcataca attacattag
    27901 ctttacgatc acgcatttct ctaataactt cccgcgttgc tgctacagtt aaactatcac
    27961 taataaagaa gataactggt gtattttctt caataagcgc aattgggcca tgcagcattt
    28021 ctccgcccgg ataagcttca gcatgaatat agctagtttc ttttaattta agttgtgctt
    28081 ctacagcaac atcatagtca aacccgcgcc ctaagaaaaa gacgtcgcct gacttataaa
    28141 tttgatcggc aattgcctta atcgtatcac gacgcgagat aatgttatag agcgcttcaa
    28201 taactctttc taatgctgca attgtcgtaa ttttatttac taagcgcgcg taaagcaaag
    28261 aaagaagtgt aatttgcgcg atataagcct ttgtactagc aacactaact tctgtaccag
    28321 cgtgtagtaa taagcgataa tccgctgatt gataaagtgt agaaacttca acattagtaa
    28381 ttgctaaaac atcagcgcca tatgctctaa ttgtgcgtaa acatttaaga atatccgctg
    28441 tttcacctga ttgtgaaatt aaaacatata atgtgtcttc acctgattga tacggataaa
    28501 aaatccattc actagcgatg aaaacgtcaa ctttcttatt aaagtttcgt agataacgtt
    28561 gtcccactaa agcggcattg taactagtac cagccgcaat aaagacaatc gttcccgcat
    28621 caataattcg tttaataagc cggtcatcaa accgatattt cgttccatca aagtaatggt
    28681 tgataaggcg acgaataaca ccaggagctt cttcaatttc tttaagcata tagtgtggat
    28741 aatcacctaa ctcaagtgtt ttttcactaa ctgacacttc ggtaaaagac ggttttcttt
    28801 gtttaagttt gccgtcatat aaaaccatat tatcacttgt taaatagcct aactctccgt
    28861 catttggata ataatattca tcggcttgat cggcaattgg ggtgtaatct gtacaaatga
    28921 ccatcccgtc atgattcttt ccaataatga gcggaacacc atttttagca aaaaatattt
    28981 tcttacaatt accgttaaag acagcaagca aggcataatc accttctagt tgttgcatag
    29041 cgcgatggag tgtccgtagt gggtcttcgt tttcaacagc gccgtcttca attaaattag
    29101 cgacaacttc aacagcggaa ctagatctaa aactatatcc gcgttttaca agtcgacgct
    29161 taatcatttg aatattatca ataagcccat taacaacaag ggtgacataa ccattttcac
    29221 tagagagcgg ataaacattt tcaagaactt gatgcatgta tgttgaccat ctagtgtgag
    29281 caatccccat ataaccatca acttcttccg gaataagtgt gactaattca tctacttttc
    29341 ctactacacg atataaatgt tggccttctt tattgcaaaa aagaactcct gtggagtcat
    29401 catctttgaa ctgaagtaag tttaacgcac ttaacagttt ctcttttaca cacgaattac
    29461 caactcttcc tataaatcca ctcataaaga ttccctcact tttggtatta atagtttaca
    29521 ctattacgca atgtaaatct tataatttgt acatattcat taatttttta aagatatttt
    29581 gctcttgttc tcccactttt cgtatttcag ctttaacttt ttctggattt agttttttaa
    29641 caagatgcgc taaatcacga atcgcgttct cagttaaggg gtcaatattt aatttaaact
    29701 tagcaacaaa tcgcaatgcc cgaataatcc gcagcggatc ttctgcaaaa cgggtataag
    29761 gattgccaat aacgcgtaaa gtatttgtat ctaaatcttt taagccatca caaaaatcaa
    29821 gcactcgata attttcatca atataaagtg cgttaatcgt aaaatcacgt ctaatataat
    29881 ctttagcggg gtctgttaca aactttatat aagcggggtg gcgactatca atatattcac
    29941 cttcttcgcg caaagttgta atttccacat gttcatcttc atactttaaa cttactgtcc
    30001 caaacttttc aaaccgataa tttgcgtcag gcaaaaattc atgcatttcc gttggtgtag
    30061 cgtctgtcgc aaaatcatag tcgtcgaccg ggtagtttaa aagaaaatca cggcttgtcc
    30121 ctccaaccat gtagagttta aagccgtgct tgttgaaaat attagcaatc tcgtcaaaaa
    30181 actctaaata tgatttcaac atacttctct agcaaagagt tatttattga actttttctt
    30241 tgagaagttt tgaaggtttg aaaacgatag tacggccgct tggaataaca atttccttac
    30301 cggtttgtgg gctaacgcca cgacgttctt tacgttctct tgtttggaaa gtgccaaaac
    30361 ctgtaagttt tacatcttca cctttcgcta aggtttcgat gactgtgtca caaaataaat
    30421 taataatttc ttccacagaa cgcttagtaa tcataagctc atcggcaatg gcagcaatta
    30481 tttctgcttt gttcatatta ttttctcact cctttgtttt ttacaaagta gcataattat
    30541 ataatttata caagaaatat ttaatatttt attgaaaatt attgcgatgc cttacgactt
    30601 tattgatttt tttacactta aaacaaccct aaaaatgaac aaatgtttaa tctcatttac
    30661 gctcacggaa tataaatttc agtggtgtac catcgaacgg gaacgagtca cgaatttgat
    30721 tttctaagta ccgctcataa gagaaatgta gaaaagcagg gttattcaca aagaacacaa
    30781 atgtcggtgg tttagtgctt acttgattag cgtagtacac ttttaagcga ccgccattaa
    30841 acactggcgg gtcattatac aattgagcgc gatggataac ttcatttagt aaacttgtct
    30901 ctacacgtcg acgatacgct tcattaacat agtcaatttg gtcaaagatt tgatctactc
    30961 gttgtttagt taaagcacta acaaaaacaa ttggagcata ttcaacaaat ttaaattcat
    31021 ttctaatttt atttgtccat tcattcattg ttttgtcgtc tttagtaata gcgtcccatt
    31081 tattaacagc aataataatg gccttttttt catcaactgc ataaccaaca acgtgtttat
    31141 cttgttcgcg aatacccaca acagcgtcaa tgacaaataa aataatatca gctttagcaa
    31201 tcgcgctgaa agcccgaagc gctgcatatc tatcgacact ttcataaatt ttgccacgct
    31261 tttttagccc agcagtgtca attactacat aatttttatt gtttctagta aaaggcgtat
    31321 caatcgcgtc acgcgttgtc ccttcaacat ttgtaacaat gacgcgttcc ctatttagta
    31381 tcgcgtttac taaacttgat tttccgacat taggttggcc aataacggca aaagaaatta
    31441 catcctcttc ataatcatct ttttgttccg gtaatacttt aataatttca tcaagtaagt
    31501 ccccaatccc aatcccgtgt tcaccactga tagcgattgg ctcacctaag cctaaactat
    31561 aaaagtcatg gatgtaatga tgatgttcaa ttccgtcaat tttattaacc gcaacgataa
    31621 ctggcttatt agcgcgatat agtaaattag caatttcacg gtcgtcggtt gtaacaccaa
    31681 gcggaccgtc cccaacataa acaataacat ccgcttcttc aatcgcaatt tcaacttgcg
    31741 ctctaatttg ttcctggaat gctgtcttag caatttcaat tccgccagta tcaataagat
    31801 taaagcgacg cgttaaccac tcagcacttc catagagccg gtcacgggtc accccgcgtt
    31861 catcatgaat tatcgctcgt ctttcaccga tgatacgatt aaaaaccgtt gatttaccaa
    31921 cgtttggtgt tccaacaatt gccacaactg gtaatgccat tatttaactc ctaatttctt
    31981 atcaacgatc ttgataattt cagcgacaac ttcatcaatt gataagttgt cagtatctaa
    32041 ctcaatagcg tcatccgcct ttttaagtgg cgccattgct cttgttgaat caatatgatc
    32101 acgcattgct agttcttttt caatatcttc taaactagtg gcaatccctt tttctaaatt
    32161 ttctaagtat cgtcttttcg cccgcactgc aacagaagcg gtttgaaata tttttacaaa
    32221 agcgtctggt aaaacatatg taccaatatc acggccatcc attacgacac tattaacttt
    32281 agccatttcc cgttgttgtt cagttaaaaa taaacgaatt ttgggatatg aagcaatgta
    32341 gcttacattg cctgaaacac taggttcacg aattgcttta cttacatctt caccattaac
    32401 aaaaacttta taatcagcag ttaaccttat cgttacttca ggaattaaac tatttgatgc
    32461 cgtttcatct tttggatcaa caccgcgttt taatacgtac cacgtaaaag cgcgatacat
    32521 agcaccagta tcgatataga caaaattgta atgattcgct acttttttag caattgtact
    32581 ttttcctgct gcggcaggtc catcaatagc gatagcaata ttatttattc ccatatatta
    32641 aaatcatgaa aataatacca gcaataataa ggagcaatac gataatagct agcccatata
    32701 atatgcctat tggtcctttt tttactgcgc tttctttatt ttcatttttc ctttcatatt
    32761 cactaagtaa agcttcagcc tttgtcagcg gtggtgtttc agtcttttta ataggagtat
    32821 tttcagcttg ttttttccgt gattcacgca ttttttgttc aacttcaaaa tcattcgtaa
    32881 tactctcacg atattttgcc cagtactcta ctctcgtttt actttctcta cgctttgcca
    32941 tactgtattt ataataatat aaatatacat aaaatgctta cacgacaatt ttataattat
    33001 cgtttccaat atgatttgaa ttgatactct caaatagtta taatattatt catcaggagg
    33061 aaattatggc aaaaaaagtt aaactcgata aagatttatg tattagttgt ggcgtttgta
    33121 acgcagtttg tccttccctt ttcgattggg atgatgacgg aaaactcaaa gctctcgttg
    33181 aagaagttcc agcagattta gaagatgctg cagctgaagc cgaagcttcg tgtccaactg
    33241 gtgcaatcac cgtcgaataa gttaagagaa ttaaaaaaat accagcagcc acttcgggtg
    33301 ctggtttttt tatgctaata aaactgattt ataaagcgac taattcgttt atatacaacg
    33361 agtgttagtc cgccgataat tgtattttta ataaaattaa aagggatgac ccataaaata
    33421 aattgctctg gtgtaaaagc cccatattta ttacttaaaa tcattgccat tttaccgtta
    33481 aaaaacattt cattgtaaag atgataaata acgaagtaat tggcaagact gctaactaaa
    33541 atttgcgcaa taaaggcgat gcctaaacca agaagcgccc ctttgaaagt ccgccgatat
    33601 ttatatatga ttgtggcagt aaggacaaag ctcgatgaat atataatatc agcgacttca
    33661 ccgaccattg cagttgatga catcggtagt tttactagaa acctcactaa taacacaagg
    33721 aatccgctta aaggcccgta agcaaagccg gcaataagtg caggaatttc actaaaatta
    33781 atttctaaaa aattaacaag cgggaaaatt gggaacacaa acgggggaac tttagtaata
    33841 taaagtaaga ctgaaatagc ggcaaatatt gccgtaccag cgacagtaac tgttcttctg
    33901 cgtttacatt tttgttgtgc attagtttct aaattatcat tttgacaatt ggtgtaattt
    33961 ttctcttttt ccataaaaaa tagccctctc tattcagggc tacacataaa agaaacaact
    34021 tcttccatcc agactttaac tgtcgccact agaatttcac tagttcagct tgcgctcgcg
    34081 gggtataccg ccggtgggga ctttcacccc gccctgaagt acttttataa tagtagttat
    34141 cggtaataaa ttcaagataa atgattattt aaaatgtttg tttttaaacg ctagtgcttc
    34201 ttcttcggta ttaaacacaa ttgttaattt atttttcatt tgaataacaa aataatcctt
    34261 accattatat gtcgcagtat ttggtgcttt tattaaggct tctaattcac ggacagagag
    34321 attattaaca cgaattaatt ctaaatattc aagacttttt tcgcgatcaa atgagattaa
    34381 agcccgtgca tgtccaaaag ttatttgctc gttattaagc gcacgaataa cttctggctc
    34441 aagctttaaa agtcgtaaaa tatttgtaac ctgcgacctg cttctgaatg ttaaatccgc
    34501 aagtgcttgg tgagaatagc cgtgttttgt cgataaaaca ctaaagatat acgctaattc
    34561 taaaacatta gtaccaagtt gatcacgcgc tttcgccaat aaaattaata aggtttcttc
    34621 atcattataa ttcataacga tgacgggaat ttcttggatt tctgtaagtc ttgctgccgc
    34681 tagacgccgg cgtccaataa ttacttcgta cttatcacct tttgctctaa ccacgagtgg
    34741 ttctagtaaa atattacgct gataattttc aacaaagcgc gcaatttttt tacgggacaa
    34801 agtaatcttt ttaataaaat tattgtctac tattaaatca atcggcaaca tttgaatcgg
    34861 ccgattcgta tgtgctttcg caagttcttc taaaacattt gattgcgaat atttagaaat
    34921 taaatctgaa aggtgttctt ttaacaaatt attcttcggc tcgttcttca ttctccataa
    34981 tctccttagc tacatttaag taggctaatg aacctttcga agtcggttta tataaaatgg
    35041 ttggcagcat tttagcactt gcttccgcta gagcgatatt ccgcgggatt ggtgtcataa
    35101 atgttgattc tttaaataga cctctaattt gttggctaat ttcaattcct agtctagttc
    35161 tttcgtcata catcgttaac agaaaacctt ctattttcag ttccgaatta tgtgaggctt
    35221 gaacttgttt aatcgtcgct aaaacttggg caacagcctc aagcgcaaaa tattcacatt
    35281 gcacaggaat aattacacta ttagccgcca ctaaagcatt agtacttaat agtccaagtg
    35341 aaggcgggca atcaataaat atataatcat aacggcgatc aatttcttta atttttgatt
    35401 gtaaaacggt aaacgggtgt tgcggaatcg tgctaatttc cacatcagtc cgcgccagta
    35461 aaagattagc gccaataata tctaaacttt ttagtggtgt gcgataaata gctttattaa
    35521 tattaatgcc cttgaaaaaa gtatgataaa tcgtattttt aatcgtcgca ggatcaagcc
    35581 caagcccacg gctactattg ccttgcgggt ccatatcaat taaaagtacc ttgcgcccaa
    35641 aatgtgcaaa agaggcactt aaattaattg cggtcgttgt tttgccaaca ccgcccttct
    35701 gatttgcaat tgcgatgatt ttagccatac tattccttgt aataagtata tcaaatatac
    35761 tttaaagtgg atttttctta atttgtgcga atggacgcgg atatttttta ggagtacttc
    35821 cctttttaat aaagcgagcg ttgacccgca tactaccgtc acttggtaag aaatgtaaat
    35881 ctgtttttcc aacttcaact ttaagggtgt aaattgcatt ttcagcgtca cttaattcag
    35941 cattataatt tcgccccttc atcatcaaag cactgccatt gatgcgtaaa aacggtacgc
    36001 ttagttcaag cattatactt agcgaagaca cagcgcgact tgtgactaaa tcatattcac
    36061 cgcgtttact tgcggcataa tcttccgcac gttcattaac aacttcaaca tttgtaagtt
    36121 ctaattcttt aacgacagta cttaagaaat tacaacgctt cttaattggt tcaagtaaag
    36181 ttatttgtaa ttcaggataa gcaatcgcta aaacaattcc tggaaaccca gcaccactac
    36241 caatatctaa taatgtttca ttgcggaaat tatatgtttc gcgaagtaat aagcaatcta
    36301 aaaaatgctt ttcttcatat tcgcttgggg caattgcggt taaattttgt accttattat
    36361 gctcctcgat aagtgcagca tactttgcaa attgcacttt taacacttca gcattttcgc
    36421 taaaatgctc gtctaagtac tgcaaaaatg ggttattttt catttttacc atacctcctc
    36481 aaatatgtca ttaatataat aatatcgtta ggattaacac cactaatacg tttagcttga
    36541 ccaattgtaa gtggtttaac tttagctaag cgctgccgcg cttcgatccg caaaccatct
    36601 aaatcatcat aagtgaaaga agcaggaatt tctacttctt caagcttctt taaacgcttt
    36661 gcttcttggc gttgcttatt aatatatcct tcatatttta tatcagtttc tagttgaaat
    36721 aaatcattgt ggttaagacc ctcaagtgtt aagtgtccta aaagctctaa taaatcatct
    36781 agtgtaactc gcggtctttt aataagttca gcaatggtta atccgcccga ataattagga
    36841 taaccaacac tttcgagaaa ttcaacgaca ggaatatccg cgcttacttg tgttgtttct
    36901 aaaattgctc gagcatccgc tatggctttt tgacttttta aaaacttcgc ataacgatca
    36961 tcttttacta agccaacttg tcgcccaatt tcggtaaggc gcgaatcagc gttatcatgt
    37021 cttaagagta agcggtattc agatctactt gataataaac gatatggctc ttcagtgcct
    37081 tttgtcacta agtcatcaat cataacacca atgtaggcct catctctttt caaaattaag
    37141 ggtggcttat tatctatttt caacacagca ttaattcctg ctaaaagacc aagtcccgct
    37201 gcttcttcgt atccacttgt gccaataatt tgaccagcgc aatataagtt atcccacttt
    37261 ttaagttcta gtgttggttt aagttcaagt ggatcaattg catcatattc aatcgcataa
    37321 ccatatttta atatttcaac attttctaaa ccttttagac tgcgaatcat tttttcttga
    37381 acttcaactg gcattgaagt acttaatcct tgtaaataca ttgaatcagt gtaacgtgat
    37441 tctggttcga tgaataattg atgacgttct ttatcactaa agcgcacaat tttatcttca
    37501 atgcttggac aatatctagc acccgttcct gttacaagcc cgctatacat cgcggaatca
    37561 cctaaatgtt cattaataat tgcgtgcgtt tcaggtgtag tgtagagtaa gtggcagagc
    37621 acctgttctt caagggtgtg atagcgctca ctcatatagg aaaaatttaa gtagccttcc
    37681 atccctggtt cttcaaccat cacactataa tcaatcgtat cttttttaag tcgcgctggt
    37741 gttccagttt taagtctaaa gattttaagc cccattttct ccaaatacgg cgataaacct
    37801 ttactagaac ggtctccttc tggaccaatt tctttaacag tgtgaccaac taaaattgtt
    37861 gattccatgt atgttccagt tgttaatatt gttgcctctg ccaatatttc ttcatcgttt
    37921 tccaagcgga ccccatacac tttttctgtg tcatgaatga ccgaaactac cattgcttct
    37981 tttacagtta aatttggagt atttagcaca atgctttgga cataacgtgg ataaccaact
    38041 ttatcttctt gtgctcttaa acaacggaca ccaggaccct taccagtatt taaaatttta
    38101 atttgtagtg gatcgtgatc agcaaaaata cccatcatcc cgccaagcgc gtcaacttca
    38161 cgcaccacaa tccctttagc gctaccacca atattaggat tacatggcat attggcaatt
    38221 tttttaatgt ccattgttaa aagcagggtg tttttaccca tatgggcact agctagcgca
    38281 gcttcaaccc cagcgtgacc accgccaacg acgattatgt cataatcaaa cttcattaac
    38341 caagtgagcc ttccatgtct agtttaatca gacgattcaa ttccacagca tattccatcg
    38401 gtaattcacg agagaatggt tcaataaagc ccataataat catttgcgtt gcgtcttctt
    38461 cacttaaacc gcgactcatt aaataaaata actgttcttc actaatttta cttaccgttg
    38521 cttcgtgttc aataaatgac tcgttgttat gtatttcatt agtaggaatt gtgtcacttt
    38581 ttgatatccc gtctagtatt aaagtgtcac attcaatatg tgtttgtgaa ttaggagcat
    38641 tttttgtatg tcgcactaaa ccgcgataat ttgccgtgcc gccactatgt acgactgatt
    38701 tagaaataat ggtacttgat gtatttggtg ctaagtgaat cattcttgca cctgcatctt
    38761 gatattgacc tttccctgca acagcaatcg aaattgttgt gccgcgtgct ccttcaccgc
    38821 gtaagataca agcaggatac ttcatattaa tagagctacc aatattccca tcaatccatt
    38881 ccatctgtcc gttttcataa acatctgctc tttttgtaac gagattaata atgttatttg
    38941 accagttttg aatcgtagtg tagcgacatt tagcgtcttt atgtacaata atttcaacaa
    39001 cagcagcgtg taatgagtct tcactataaa ctggagcagt acaaccctca acataatgaa
    39061 cacttgcccc ttcatcgaca ataattaatg ttctttcaaa ttgacccatc tttgccgcat
    39121 taagtctaaa gtaactttgg agtggtttat ctagttccac accttttggc acataaataa
    39181 atgaaccacc gctccataca gccccattta aggctgtata tttattatcg gtatatttaa
    39241 caacggtatt aaaatatttt ttaaatatct caggatgttc tttaagtgcc tcatcagttg
    39301 atgaaaaaat cacacccttt tctttaaatt cactaatcat actagcgtaa acaacttcgg
    39361 aatcatattg cgtcgccaca ccagcgagaa atttacgctc agcttcagga atacctaact
    39421 tttcaaaagt ttctttaatc gtgtccggaa catcgtccca cgagcgtgcc tcatctttta
    39481 caacacggtt aaaataagta taagaatcaa aatcgagatc ttttaaagat ggtccaaatt
    39541 tagggttttt cattaattca aacgcgcgaa gtgatttaag tcgaaactca agcatccatt
    39601 ctggttcttc tttaaaggca gatatttgcc gaacaacatc ttcgttaata ccaataccag
    39661 tactagcaat tgattcttgg tcatcggaga aaccataagc ataagtacca aattttaatt
    39721 catcagcact agtcattgtg attttcctcc tctttatgtt ttgtaattaa ttcagcaacc
    39781 ccgtcccacc caattgtggc acagcgaatg cgacttggtt gccggactgt atttgcaaat
    39841 acaattgctt cattaagaat atctatgtca taaggttcgc tgttgagcat ttttttgtat
    39901 tccgcaatta ttttcaagac agcatcaaca ttcattcctt caatcatttc aaggaggatt
    39961 gaggtagagg caatcgaaat agtgcaacca aatccatcaa aatatccggt gataacttta
    40021 ttgtctttaa cttttaaata aagtgttaaa ttatctatac aaccactgga agtaaggtga
    40081 atttgcataa attcatccgc attttgcggg gtttccttgt gataagggtt ttcataatgc
    40141 gttaaaattg tcgcccgcat aagctcgtta ttaaaaatag gcatctaaat aattctccgc
    40201 tgttctagct gcctcaacaa aggcatcaat ttcttctttt gtcgtataca aatataaact
    40261 tgcacgtaca gttgccactt cgcgtaaatg ttctttcagc aatttagcgc aatgttctcc
    40321 actacgaacg gcaatattct tactattaaa atatgtagca acatcttgtg caaacacatt
    40381 tttaacattg aaagtaataa taccagtttc agcattttcg ttatataaaa taatatgatc
    40441 catttctttt aatttagaaa cagcatatgt tcttaactta tgttcatgtt tactaatatt
    40501 ttcaatacca atcttattaa tatattcaat cgcggcatgt aagccataaa tcgcctcgat
    40561 gtttaatgtg cctgcttcaa acttaagtgg gactttaaat aattcataat tcccttcttt
    40621 atcataacta aaattcatgc ccccgcccgt ttggtaagga tccatctttt ctaataattc
    40681 ttttttacca taaagtacac cgatacctgt tggcgctaac attttatgtc cgctaaacgc
    40741 taaaaagtca acatctaagt cttgaacatc aactttcata tgcgggacgc tttgcgcacc
    40801 atctaaaaca ataatagcgc ccttatcatg cactattttc gcaatttgtt taataccatg
    40861 aacatgacct aaaacattgc taacatgaag tacactgaca acttttgtat gttctgtcat
    40921 atatttttcg acaatttctg gagtgacttt cccgtcttca ctaagtggca tatagacgat
    40981 tttagcacca gtttttttgg ccacttgcat ccagggtaag gtgttagatg cgtgttcagc
    41041 aattgataat aatatttcgt caccctcttt taagaacttc tcaccataac acttagcaac
    41101 catatttaat gaggccgttg tcccttgggt aaaaacaact tcttgcacat gtttactatt
    41161 aataaattta tgaattaaag cgcgcgtttc atcaactctt ttatcgacta aataagcatt
    41221 ttgataatcg ccgcgcccac tattagcggt ttcttttgat aaataattag tcactgcatc
    41281 gattacaact tgtggtttat atgttgtagc agcgttatct aaatatattg ttttgttatt
    41341 atcaaaaacc ggaaaatctt ttctaatttt ataaacgtcg tacattataa taccctctca
    41401 agcatagtaa tgcagttctc ttttaaaact ttatcttcaa tataatttaa tatcggcatc
    41461 aggtaacctt ttgtaattaa aagtttagct tctttttctg taagtccgcg ggactttaag
    41521 taataaagat gttcagcatt tacttgtccg acagtagcgg cgtgacttgc agcgacgaca
    41581 tcattatgat aaatattcaa aattggatta gcctctgcat ggctattttc gtcaaaaaca
    41641 ataatgcgtg cggtttgatg ggtttccgcg cccttcacat tttcaaaaat tgttgattca
    41701 cctgtaaata taagagttga tgcattaagt actacaccgt agttatgcat atcagcaaat
    41761 gactcatttc caaaatgtga aaacgagata ttaaatactt tcttatcaat attttgtgaa
    41821 taagtagcaa gatgccattc tgctctagct tttctttctg atagtttcgt ttcaatattt
    41881 accttgcgat taccattatg gaaatcaggc ataattaatt taacttcagc accttcaagt
    41941 aaaataattt cgcgatcaat gctaagattt tcctcactac acataaaaat tacttttccg
    42001 cgcgtatatg gcgcaagttc ataggtaatg aactttttgt tttgccgtaa tacaacgaca
    42061 aaatcaatgt cttttgatga ttttaatata actttagtca cagaagtagg taagtcgttt
    42121 tcgtcaaaaa tatgataaac agtatcgttt tcacttaaga tgagcgtgtc atcattaata
    42181 ttcattttat ttattaataa cgctttaaaa acactcattg tttacttctt tcattgaccg
    42241 cacatatttc taatacaatt gggcgtttgt cttcgcgttc aatattaatg ttgtgttcta
    42301 cttgaatcca ttcataacct tcttggtcaa tgcgcttaat gacatccata ccgccttcaa
    42361 tcgctactcg accattaatc attacaatgg cgcgcgttgg attaataagt tcatatagtc
    42421 ttgcgtagtg actaataacg agaaatgttt tatctttttt ctcttcattt atttgttgcg
    42481 agacaattcg aatcccgtca acatcaagac ccgaatcaat ttcgtctaac attgcaaatt
    42541 caggttttaa taatttcatt tgcaaaattt catgacgctt cttctctccg ccagaaaaac
    42601 cttcatttaa attacgcgag gcaaggtcgt ggggcatttg catttctttg cttgctttat
    42661 caatcacgcg gaaaagttca attggtttaa ttggtttttc acgttttgta ttgatagcgg
    42721 cgcgtaagaa atcactaatc tgtacaccag gaacctcact tgggttttgc attgcaagaa
    42781 aaagccctgc tctagctctt tcgtcaacac tcattgctaa aacatcttga cctttatata
    42841 aaatttcacc tttcgtaact ttataattag ggtgacccat aattacttgt agtaaggttg
    42901 atttgccatg accattagga ccaagtaagg ccactacttc gccacggtta atttttaagt
    42961 taacaccacg taatatttca atatcattta cggatgcatg tagatcttta atttcgatta
    43021 ataccatctt tacaaactct ctttccgttg tatgcatata aaagatacct taaaacacct
    43081 aaaaaataaa ctgttttact atcctatttt tatttaaaaa taacatatca ttgttttata
    43141 tgcttattta ttaattttta tttcagtaaa aaaggatttt atattcccta acatccttag
    43201 ttttatcatt tattgcaatg tttatgtttc acctccgttc atcattattt atttcattca
    43261 agtaaactat cctaacaaca attagtatta cttgaattag ttttattata ctattataat
    43321 tatagtgtta gcaacggcta actggtaagg aaatatgaaa acaacattaa taatcccaaa
    43381 tattggttgt ccgaattgtg ccatggcaat tactgagcac tttaaaacat taaacatcga
    43441 agcaaaagta aatgtcaatc agaaaaaagt gacctttaca tatagtgata aagattcttt
    43501 caccatcata ttaaaagagt taaaagcgat tgggttccct gctgcgccaa ttgattacga
    43561 tgaaaaaaga cgtcggaaat atgaaaaagt ccgtttaata gttgcgacaa ttttagtgtt
    43621 accacttgca tacacaatgt ttgataattt tggattaaac attgtccccg atattatgct
    43681 aaacggctat ttccaaatgg gttttgctgc aatattgcaa tttatttttg gatttagatt
    43741 ttataaaaat gctttttatc aaataaaaaa taaaaattta ggaatggatg ttttaatcgt
    43801 tttaggtact tcagtagcgt tcttttatag tttgtattta actattttta cagataaaac
    43861 tcaattgttt tttgaaacga gcggtatgct tatttggatg gtaacaattg gtgattattt
    43921 agaacatctc tcacaagcaa aaacaacgga tacattaaaa tcattaatgg cacttgcggt
    43981 tgatgaagtt aggcttataa aaaacggaga ggaacaaata gtttcactta atgatgtgaa
    44041 tgttggcgat gttataaaag tgcttgccgg cgaaaaaata gctttagatg gtgaaattat
    44101 tgatggcgct agttatattg atgattcagt tataacaggc gaatcaatcc ctaaatatct
    44161 tagtgtaggt gataccgctg tagggtctac cactaattta tctaatacaa ttttgattaa
    44221 agtaagtgct atcggcagtg atactgtact agctaaaatt attaaaacgg ttgaagaaac
    44281 ttcgcttatt aagcccaaat tccaaaaagc tgtggatgtt attgctaaat atttcgtttt
    44341 tattatttta atgattgccc ttgtatcatt tcttgtttat caatttattt tagggaaaga
    44401 actctcaatt tctctcgagg tgactactgc ggtcttggta gtttcatgtc cttgtgctct
    44461 tgggttagca acaccaactt caattgcagt tgcaagcgga ttagcattta aaaataaaat
    44521 tctatataaa ggcggtgagt tttttgaact cgccaataaa attgatgcga ttgcttttga
    44581 taaaacagga acccttacta aaggtgattt aatagtcagc gattatcttg gcgatattag
    44641 atatttaagt tatacaaaag gcttagaaat ccattccact catccaatcg gaaaatctat
    44701 caataactat cgaaaagata ttattccttt tgcaattgag gaatatgaaa taattgatgg
    44761 caaaggtatt aaaggcacat atttacaaga tcaaattata gtgggttctg ctacttttat
    44821 tgctgaagct aatattagta atcctttctt aaatgaattt gaaaaattta caggtgaagg
    44881 taaggtcgtc atctttactg cgattaataa aaaggtcgta aatatgatta ttttggaaga
    44941 tgaaataaaa gctgaaagtc agactttaat tacagcactt aaaaagatga acatcaatgt
    45001 ctacctaatt accggcgata atgaaaatac cgctaaatac ttagcaaata aagttggaat
    45061 tgagaattat tatagtggtg tgttaccgaa tgaaaaagct catatcatca aaaaattaca
    45121 agaaaagcat agtgtagttg cttttgttgg tgacggaatt aatgatgcac ctgccctaaa
    45181 acaagcggat gttagtttct ctgtcagtaa tgcttctgat atcgccactg atacagcaga
    45241 tgtaactctc ttagataacg atttaaaact agtgttaatt gctattgatt tatcaaagaa
    45301 aacaacagcc aatattaaac aaaatataat atgggcattt ttatataact tagtaatgat
    45361 tccgcttgca atatttaata tcgctacacc actagtaggt ggtttcctcc atgttatttc
    45421 tagtcttctc gtcgttttta atgcactgcg tctcaattta tataagcaca aaataaaata
    45481 atatcttcgt tcctattaat tttgatttat taattatcat ttttgaaaat tcaaacttta
    45541 gaaaatacac aaatatctct tttgcttttt cataatattt gtataaacct taaaatatca
    45601 tgaaaaattc aatattttcg tattctattt ttatataaaa ataagttatt ggttaattta
    45661 tcgactatta acgcttattt ttgttgaaaa ggttggaagt taagtataga atataaaagc
    45721 atattattag gaggtatagc attatatgag aaaaccaaaa cgcattattg ctggtttagc
    45781 gctatcggca ctacttctta ccggatgtag taaagattta gtcgcagaca aaaaaggcaa
    45841 tattgtgacg gtgacaatcg atggtaaccc agtacaaatt aatgcagggg atatgtttga
    45901 agactattta aaatcctctg atggactaaa ggattattac gacgccgtct atgaagttgt
    45961 ggttcgcgct ctctttaata aagaagagca aactacagag cgaaccgaaa tctatgatat
    46021 tgctgccctt agcgttaacg atgtgaaaga acaagcaaag aataacgcca aaggtggtgg
    46081 caagtacgac gatgaactta agaaaattct taagactaaa aacgtcgaaa cattaaaaga
    46141 attagaagag aaactcgcat acgatgaaat gcaagtccgc ctcaaaaaac aattctttga
    46201 tggaggcgaa ggcgcctgga tcacaaacgc aatggaagaa ttaatcgttg gtgaacgaaa
    46261 tcaagcagga gatttaattc ttgatgaaaa caacaatcct gtttacgaag gatacttaaa
    46321 caacaaactt ccatatcacg ttcgtcactt actcgttaaa gtcggtgccg caaacaacga
    46381 ctttgttacc ggtaagattt ccaaagacga tgcgaacaaa ttaagttctg ttatcgtagc
    46441 cttagcaaat catcaagaca ccttcggact tttagcaaac cgctatagtg aagacgaaga
    46501 tagtgcccgt caatttggtg acttaggcat tatgaacttc accacaaact ttgtgaatga
    46561 gttcaaatta ggagtttatg cttatgacgc tctttacaac gctgacggag ttatgactcc
    46621 agaagcaaaa gcggctaaac ttaaaattcc tggcaccgcc aaaactaaat tagacgcact
    46681 cggacttgac gaaattcctt acggcgctgc gcttaaactc gccgctgttc gtgaaatcga
    46741 aaagagcgat gctggtaaag aagtaaatga cggtgatgcc gccttcttcc cacgtaacgt
    46801 tatctttaat aaatacttta ataaacataa cattggagtc attactccaa atgaagttaa
    46861 tgtccttgac gaagtcggtg tagaaaaagc tgaatttgct gcccttcctg gatttaaagc
    46921 tcttccagaa ctcggtggta aaaaagtttt aaccgacgaa aaaggtaatg ttatcttagt
    46981 cgtgcgcgct ggttcagccg gaagttatga aggaatccac ttcatcgttg tcgaacgcag
    47041 tgctctttat gacaccgtaa acggcgttac tttaaaagac tactacacaa tcgaaacccc
    47101 aggaaccgct gctttcccaa aagacgatgc tggtaacgat attgatgcct ttgtcaactt
    47161 cttagaactt gacgcccgtg gcacaaaaga aagaatcgac aagattcgta atgaagttaa
    47221 agcatttgac ccaacaatca acgatcgcat tttccaaaag ttagtgacat atcttgatgt
    47281 gaagttccat gatgctgaac tcaaagcagc gcttgataaa tatctagcga accaatacgc
    47341 tcgttcccaa ttccaactcg accaagatct tgataaatta tggagcgaat ggattgaata
    47401 tttagatcaa caggaagtcg cacgtaccga ccgccttgtt cctgaaacat atattgatga
    47461 tttcaaggct ggtaatgact acaattacgg aggttaatag agtgaaacgt aaatatttat
    47521 taggcttagc cgtcgctgca cttctcttaa ctggttgtga caaaattacc gccgaaccag
    47581 ttgatttaga taaaactgtg cttgtcgacg aattaagcga agttgttaac aacacattag
    47641 aaaatgtcta taagaaatat catgactccg cgaactttaa acaattcgta ttagacgaag
    47701 tgttacttac gattgccgaa catgaatttg gtccatacga caaattagcc gcagacgacc
    47761 cattcaaact cgcagtcgat acacgcgcga aacattattt ctatgatgaa ctattaagcg
    47821 gttcatacac atatcgctca atctttgacg aaaagaaata cgtcgttgaa agagtatacg
    47881 gaacggaaaa tagctaccta gtggacgcta acggcaacga agtcgccatt ggtgcgattg
    47941 ataccttacc attctacaaa gaaggcttat tccttccaat cgtcaacaaa gataactttg
    48001 acgatccagc ctttaaactt gtccatatcg attactataa gcattacatg caagatcgct
    48061 atgttgataa gatttatcgc gaaaaattaa cagaaaaatt cgttaaggaa gaacaaactt
    48121 ccaccctcgg ccgtaactat gcccggaagg ttgaatatgt cgctattaaa aataatagta
    48181 accacccaac cgccgctagt gcactcgtta atgcctttat tgataataat atcttaggcg
    48241 ccgagcctgc tgacttcaat atccttgcta atgcttggag aggttatgaa ccagacttta
    48301 ttggtaatga agctgcactt ctagccgcaa ctggacttaa aaccgctgaa gttaacaaaa
    48361 ccttaattgg tgacattgaa agtgatttcg ccaaaattaa agctaaccct gacttaactg
    48421 acaaagcgat tgaagatcgt ttcactggca gtggtaaata tccaaaagaa gttgggaaac
    48481 aaattgaaat taatgaagtg cgtaaacaat cattcgtcac gaaagactgg ggtattaaag
    48541 atggtggctt tagtagtcta ccagaagcta tccgctcccg cttattcaac attggtgtcg
    48601 ctaatagtgt tgacttctta aaagacgacg ctggtgtcct tgctgatgct ggtaaatggg
    48661 aaggcgacgc cgaaaacaag aaagttgcgt caaccttcgt tcgtaatatt catgacgatt
    48721 attacctagt ccccaaaact tatgaaaaag gtaacaatcg taacttcctc ttcttcgaaa
    48781 acgatacatt ctacatcgtt aaaatcgttg aagccgttaa caccgctaaa cttgccgcta
    48841 aagatgatga cttaaattca tatgagcatt tcaaaactga agcagaaatt gcggaaattg
    48901 ttgcccatgt tactgaagct ttagcgaaac tcgatgcgac taaaactaat gcgcttaact
    48961 tctacatgaa agacttaaag attgtcttcc atgacgaaga tgtctatgat ttcttcaaag
    49021 aaaaattccc tgatatcttc gatgatgaaa aaaagaagaa ataagcatac cttctattta
    49081 ttaaataacg accgcaggtt aacccctgcg gtttttattt acagatattt gttataatta
    49141 atatatagat aaaggaggaa cactaatgag taaagattgt gttttttgta agataattcg
    49201 ccgagaaatc ccaggtgcca tcgtctatga agataatgat gtccttgctt ttcttgatat
    49261 ttccccaacc gctccgggtc acactttagt tattcctaaa gaacaccacg aaacattttt
    49321 aggcactccg cgtaaattaa tgtacaacgt catggatgtc gcgcaacgta tcggccaagt
    49381 gcaaattaaa acacttaaag cgcaaggcgt taatatttta attaataatt atccgcccgc
    49441 tggtcaaagc gtcccgcact ttcatgtcca tgtaatcccg cgttatggta aaaaagacgg
    49501 attaaaaatc gccatggttg aacaccttga tggcgattta aacttcccag cagtcgtgga
    49561 aaaaattaag tctggtttat aattattttt tcggtttata aaaactcgtt gaatcaagcg
    49621 cccacaaccg atcagtgaat tcaccgctgt ttgttgtgtt gagcgctttt tcaattaatt
    49681 tcattttggc atctaagtca tcaatcatac tcacgagttg tgcttctttt gtcattggag
    49741 ttacaggtga accgtattca agcttaccgt gatgcgataa gatgatatgt gataaaagaa
    49801 ttggcatttc acttttaatt tcaagttctt ctgcaacttt taaaagttca gcgttcatta
    49861 gtgaaatatg gccaacaagt ctaccagcaa ctgtataact agttaacact ggtccactta
    49921 attcaattgt tttacctaag tcatgcagta aaatgcccgc aataactaaa tcgcgattaa
    49981 tatgccaatc accttcatac aactctaaat aataatcaat gatacgatct gcaagattag
    50041 cgatggtaat tgtgtgttca agcaaaccgt gtgtatattc atgatgtaat ctgctcgcgg
    50101 caggataagt tataaaagct tcataacgat cactgattag tcgttccacg agccgctgaa
    50161 tatccgtgtc cgcaaatgaa gagagtaaac gattaagttc cgcagttaaa gtttcaagtt
    50221 taaccgggct tgccattgta aattcatcaa gtctaacctc tttaggattt aggacttcac
    50281 ccgtcatgac tttcagttgt aaattcgaac ggtattcaat aacatcagcg acaacccgca
    50341 caaagttacc ttgtttaaat acttctacat cattttcatt tgcgtcccat acccgcgctt
    50401 caatttggcc tgtattatct tgcaaactga ccgttagata catttgaccg ttatttgata
    50461 cacctttagt tacattcgtc actaaataat tttcaataac acgaatgcca tcatttaaat
    50521 ctttaatttt cataactata atcctccaac aatttacgaa ttaaggaagc gggataaccc
    50581 gcgataacta atttattata caacacttta gtgtcacctt ttgccttctt ttctagtttc
    50641 actagtgctt tcttaatatt ttctgtttca tcgatataat cttctacatt aaatttcgct
    50701 aaaacttcat caatattatc actttcaaag ccgagactat ataatttagt tttaagtttt
    50761 tgataagcac ggcgcagtga ttcattacga tacgatttta gtaatttagg tataagtatt
    50821 tcaagttcac gtcgctcgtc acgtggcgat tgcgcaagag catccttaat aacatagggg
    50881 tgaataccac gccttaataa atcttgctca atagcacgag tagagcactt tttaacatta
    50941 attaagtact gtattttatc aagtgcatac cgtaagtcat taattaaatt aagacgctta
    51001 agtttatcga ctattaaatc aataacttcc tcatttacac accttttgcg gagtttttca
    51061 cgtatttcat gacttgtgta aggtcttctc gctaataagt tactagcgta actaaaatgc
    51121 ttagtctgtg cttcatattc tttaattttc gctaagtctt ttagtgataa ctctttgcca
    51181 ttataaagaa aaaactccgc aattgtttct gcacttacta atagttcttc accattatca
    51241 aaagtgatat tgtattgctc tttcttcaat gtgatattta taattttaac cattaccaac
    51301 tattacgaag ggcccttaaa tagacgtgct cgcatctttc tacgaaacgg tcattactgt
    51361 aataatcaac tatattgttt gcttcatcta atatattgcg aatttctttt gtggataaac
    51421 tgcgaatttt cttcaccatc tcaccaaagt cttcttcact acggaaaaag aaacctgatt
    51481 caccgtctct aatcaccccg acaagccctt catcaaaacg acaaagtaaa aggcgttctg
    51541 cagccatcgc ttccataaag gttaagcctt gggtttcact aattgaggca ctaacaaaca
    51601 cttcccctaa atgataatac ttgccaatat ccgcaggtaa gactttgccg acaaaatgga
    51661 caaacggagc aatttgtaat ttttctgcaa gttcttctaa ttcaggaatt gccggtcctc
    51721 cgccaacgat gatgagtcta gtattatgat ttagattctt tttaacataa agtgcataac
    51781 ccctaattac gacatcaata cttttttctt ttgcaacacg tccaacatac actaagttaa
    51841 aagtgccgtc taacccgtgt tcctttttat attcatcaag gaaaggttta tcttcaggat
    51901 tatacataaa ttttgaaaaa tcgacacccg acggaataac atttatataa cgctgaaccc
    51961 catattcgcg aagtgacacc tttgttttat cacttggtac aataaattca tcagcaattt
    52021 ctgcttgcca tcgtgaaaag cgacggataa tagctttagc aaatgtttcc atccgtcctt
    52081 ttgtcgcgta ataagtataa tcttcataca ttgtgtgata tgtatacaca taaggaatct
    52141 tttgttgacg agcaaaactt tttccaaata tacccatccc cgcttcattt tgaacatgaa
    52201 cgatatcgat tttaagcttt tttaaaatct tatgtgcttt acggtttggt cccgcaaagt
    52261 tgtaaccata gagttttttc atttcaattc cgggaatccg caaaacatta tcgataaatg
    52321 ttacttcttt ggaaaaagga ttagaagtaa caacaacaca gtaatgaccg cgatcgttaa
    52381 gtgctttaaa aagattataa ctagaaacgg cgaccccatt agtgtcaggg acaaatgtat
    52441 cagtaaacat tgcaattcgc attattcttg atcctcttta tgattgcgcc atcttcgttt
    52501 tttcttaggt gataaacgac gctgaatttc tttaattgat aattgacttg tggcatacat
    52561 cgtcacacta gtttcacgac gttcagcaaa ggtgtaactc ttaacttctt caaatgtttt
    52621 aacgtgaccc gtttcatcaa caatttcttc catactcgaa cgatagaagg ccgtcacaaa
    52681 tccgccaacg attaagttaa cataaaatgt tgccacccgc cagataagct gaactgcttt
    52741 cgtaaaggcg gcatctccac caaaaacagg ggagaagatg atttcgtaaa aatattcggc
    52801 aaacccagca gcgccaggaa ttggaataaa gcctgtaatc attcctaaga aaccagtctt
    52861 agaaattgtt tcccaaaagg aaagaccgcc atacaatgct ggatttaaca ttaaagccac
    52921 aaaatatgta actgaatagt taacaataaa ttttaagaaa aagagaacca taataagaat
    52981 tgaagctggc acgtttgaac ttaagcgccg taactcaatt ctgaagttct cagttgaagt
    53041 ataaagttta tttttacgat attcaggtct tttaacaatt ttaattttcg ctaaaagatc
    53101 gactacaaca ttaataacaa agtgatgaac acgtctcgag tacgctagta aaaatagccc
    53161 aactatcgta actaagttag ctaaaaaccc aaatgaagca aaaacccaca ctggaatatt
    53221 aagtttaaaa atactaaccg aaccaagtcg cgcaaattct ccaaggttta aaagaataag
    53281 taatccacca aaaacaacaa gcacaatttg atacaaaata aagtgcatca ccataatgga
    53341 agctccaatt gatactggca ctccttgttg cgcaaaagtt tttacctgcg caaattgacc
    53401 acctgtagca ctcggagtaa tatcattata aaattgacca ataaaagcat tcgcgacacc
    53461 gcgatgtatt ttataagtcg gggtataaag cctagcaaaa ataaataaga taatgccttc
    53521 aataaaaaac tttagagcaa ttaaaacaat aattattata atcatgcggt aatcagcttc
    53581 cgtgccacgg aacacattta ttaccgctgg gagttcattc cataatgagt aagtagtgta
    53641 cccaaaagta attaataaaa ttacgatgat attaataaga tagcgacgat cacgcttcgt
    53701 ttgcttttgt acttttaaat cctgggttgt gctaactttc tcttcgctca taggtaaatt
    53761 atagcatgaa aggacttaag tccttagtta agacaacttg aaaggtatat tcatacatgt
    53821 ttctatttct tttttcgtaa aaacttgaaa ataccacgaa tcggtttgcc atttactaat
    53881 agtaaaatag cgtctcgttg attagtgctt acttctccag aacttaaggc tgaaagactt
    53941 ctaaacggta attctttaat cgtttcaaca gccatctctt ggagtactac atcatcaccc
    54001 gctagtttgc gtgaagtctt ggccataatc ttataaattg ttcgtcccca ccagtaatca
    54061 gtcgtatcac taatgttatt agcaagtgtg aagggacgct tcctgttagc gaaaaataat
    54121 ggtaaagatt caccaccaaa taatgttgta aaatctttaa ttgatggatt aaaatcggcg
    54181 cgacgataat acatttcatt ttctttttga taatttttta aatagcttaa atcattagcg
    54241 tcatccacta agatttggaa gatctcatca gtggtttgta catttattcc gacataaaca
    54301 ttgtaattta cattttcaag cacccactct tgctttactg gacaataagc ttttaatgac
    54361 tcagtaggta agaatatatt gatcttttgt ttttcacctg gatcaagata caccttctta
    54421 aaagcgcgta attctttacg aggaccgtag agcaaactac ggctctttcc tgtgtaaata
    54481 ccaataactt ctgcgccgcg gatatgaccg atattcgtca cttccatcgt gatgttataa
    54541 ccgtctttta cttcttcaat ttcatgatgg ctagtgttaa attgcgtata acttaacccg
    54601 tgcccaaacg ggaaaagtac ctgttctcta aaagtatcgt aataacgata gccaacataa
    54661 agtgattcac gatataaaac attttgatca ctagtgagaa actctccacc aggtaatcct
    54721 ttatctaaac ttaacggata agtttcactt aacttaccag aggggttagt aacaccaaac
    54781 agtaaattat atgtcgctgg tcccgtcata ctacccgcta agtgggtaag taagatactt
    54841 tttacatcat tgatccacgg cattgtaact ggcgaaccag ttgataagac aatgacaata
    54901 ttttcattaa cttttgcaac ttcttttatt aattcattat ggctcggtgg catgtctaat
    54961 gttttacggt catagccttc cacttcataa ctttcaggca aaccaacaca taaaacaaca
    55021 acatcactat ttttcgcaac cttaactgct tcggcaattt tctttttatt tggttttact
    55081 tcatgctctg cataaccatc agcgtacaca aaattaacat cattttgtat aaaagcatca
    55141 tgaacactta ctaaagtatg cggatttatt tgacttgaac cactgccttg ataacgcggc
    55201 ttaattgcaa aattcccaat taatcctact tttgccgttt tattaattgg taacaaatta
    55261 tcttcatttc gcagcaaaac agcactcttt tcggcaattt cttgcgctac ttcaaggttt
    55321 ttcttataat cgcatttgac ttttggttta ggattatcac gcttatacat cataaatttt
    55381 aaaagcgggg taatcatttc atcaattgtt tctaatttaa tttcgccgtc tttaaccgcc
    55441 ttttctagtt cagcggtatt ttgataaccg ccaggcattt ctaagttaag accagcgtta
    55501 atactatcaa catgatccac tactgcaccc cagtcactaa cgacaagacc tttaaagttt
    55561 gcttcgttgc gtaacttttc tgtgagtaat ttataatggc gactcgcatg tcgaccattg
    55621 atttggttat atgaacacat aactgaaaaa ggttcagtgt ttttaataac attgtggaac
    55681 ggtcttaaat atatttcatt taatgctctt ttatcaacaa tggcgttagt cgtatagcgt
    55741 tctttttctt gattattaac agcataatgt tttagtgtag ccccaactcc cttactttgt
    55801 agcccacgaa ctagaccgct agccatttcg ctagttaaat atgggtcttc actaaaatat
    55861 tcaaagtttc gtccacagag cggacttctt ttaatgttaa taccaggacc aagcaaaagg
    55921 tcaacacctt gatcaataca ttcagtagca agtgtcttcc ccattttctc taatagttca
    55981 ggatcaaaag aactagcgag tgcactcgtt gttggaaaag ccgttgcagg aattacttcc
    56041 atgagcattc cagaatcaac attaactgtt ggttttcgta caccaaaagg tccgtcattc
    56101 attacaatta ttggtaaatc agtgcccaga actttttcac tttcccaaaa attagcgccc
    56161 gttaaaagtt ttaattttgt cgtaagtgat aaggtgtgta acaaggtttt taattcttca
    56221 ttaaccataa ttagtccccc tttacaaagc gtctaataac ttcaagtgtt tttggatcat
    56281 tttccactgc gcgtgaaaaa tcaaaatcaa caatcttttg ttttaatgtc tcgtctttat
    56341 ttttttgctt actatatgta agaaaatatc ccatcacctc accaagtgct ttttcgccat
    56401 cagtcgttaa gtacgcagag tgatttttat cgctttcaaa gtgaaatttg atgtttttat
    56461 gtttatcttc ttgtaacact aagttaccga taaagagatt aaaatcaact acggaatctt
    56521 tttcaccatg cacaattaaa tgcgggattt gtgtattttt aatactgttc cgtgtcgcgg
    56581 caaacgcaaa cttgccatat ctaaaagcat taaataactt aaagagtggc acaaagataa
    56641 atggagcttt tgctgaatgc ttgatgtata acccaaaatc tgtgacacca ttaaggttaa
    56701 caactttaat aatatttttg tctttattaa atggataaag atttgccgca acaaacccac
    56761 cccacgagtg accaattaag acaagttttt tacctttata ttgcggcatt gtttgtaagt
    56821 gcgctaaaaa agcatgagcg tcaacaagcg gttgatacac actaacaatg cttttacctt
    56881 cgctaaaatc acaaccagtg taatcaaatg ttaaaagtgg catttctaat gtggtcacta
    56941 aatcattaat cagtgttgta taagcttcat gccctgcccc aatcccgtga aaaaatacaa
    57001 gataagtatc gtctttaaat ccgtcccgtg tatacattct ggccattaag ttatcgcctt
    57061 tgaagttttt aaaattaaat gtttccgctt taagtccctg ataatcttca taagtgtaat
    57121 aagtaaatag cgggttttta tcacatcgca tcttcgagcg aacactagtg aaaatataaa
    57181 ttaatagtga ataaagaaaa agaactaaaa aataagctcc aataacgatt aaaatgattt
    57241 gccatgcttc cataataaaa ccctctactt gtcttattat atattattgc gaaaaaatta
    57301 tgtgaaaaac agtgtttttt taatatc
//
LOCUS       DAOSLP010000003        46744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_915_length_46744_cov_7.241599, whole genome shotgun sequence.
ACCESSION   DAOSLP010000003 DAOSLP010000000
VERSION     DAOSLP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 46744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46744
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_915_length_46744_cov_7.241599"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..656
                     /locus_tag="PK460_00570"
     CDS             117..656
                     /locus_tag="PK460_00570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOE53630.1"
                     /translation="MYENERFLRISDTASGFTDHRLKKQYFALFDWYKRNLEYEMFLA
                     NNKLRYKINQGEVYEIDFGRNVGSELNERHYAVVLHHSDVEAQNIVVVPLTTKIHFSY
                     GEAIDLGYLPEVKTTEKSYAKISQIRTVDKARIYLRPIIHTEDNKPCRHTYGPVTKLT
                     ADQFRLVIEGLNKLLNNQL"
     gene            complement(894..1976)
                     /gene="nadD"
                     /locus_tag="PK460_00575"
     CDS             complement(894..1976)
                     /gene="nadD"
                     /locus_tag="PK460_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOE53631.1"
                     /translation="MKIIVYPGSFDPLHNGHLLIARHAKREIGADLVLFLLSPSTVWK
                     KVQTSFNHRANMISAALKDEEGFELSRIEEGNEGKLNYTYLTFMRLREHYPHDELYLL
                     LGEDQAMAFDQWKHPELIAEQAKILVYKRKGSKVSNENFERFDMLEITGPLSSTSSTK
                     VRNFESIDVPSVVLEYIGNNKLYFSGLVAKRMSDERYYHSLSVASLSRLIAIANDVDP
                     LKAFKAGLFHDIGKEVPAAKSLKIMEEHFPKYLNMPLWSHHQFVGAYLMKEEYLEKDK
                     KLIDAVKYHATGHKKMSKLAKVIYAADKIEPSRGFDSSQYIAECVKDINKGFVIVLQA
                     NKDYLISKGKDIDNALTNACFKKYLK"
     gene            complement(1973..2266)
                     /locus_tag="PK460_00580"
     CDS             complement(1973..2266)
                     /locus_tag="PK460_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOE53632.1"
                     /translation="MLTKKEMRYLRGLSNKLKAQYQLGKNEITDSFLSLLEKALVAKE
                     IIKVHVLQSVDKEVTSLALEVAARLNATVVETKGKTFTLYRQNVKAPKIKFPL"
     gene            complement(2271..3398)
                     /gene="yqeH"
                     /locus_tag="PK460_00585"
     CDS             complement(2271..3398)
                     /gene="yqeH"
                     /locus_tag="PK460_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HOE53633.1"
                     /translation="MTENLKKIKKCYGCGVVLQSENEEEIGYVPSNVLKRDDVFLCQR
                     CFKTHHYSADKLHSPVVTLDFMNLIKDIKAKNALVVYVVDVFNFESSFNPEINTALSG
                     LDIILVANKFDLLPKSTNEAKLKDYVATKVEREGLNVKKIIVASSTRNYNIDEVLEEM
                     ANFRKKGDVYFIGAVSSGKSSLIKTLLRNFKNETSRYITTSIYPNTTLAVQQIPISET
                     TTIYDTPGIAINNSLLGLAEKEVVKVIVPRKEIKPRTYQLLAKQSILIGGIARFDYVD
                     GPFTGFTFYFAEGIKLTRTHIKLADRTFNNMIAHQKTRPISYLVTGVQDLDAFEFTTY
                     ENKKYDVSWNGLGFVSFKGSGRVVRIYAPRGVAISYNESKI"
     gene            complement(3373..3912)
                     /locus_tag="PK460_00590"
     CDS             complement(3373..3912)
                     /locus_tag="PK460_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOE53634.1"
                     /translation="MKKFTPNYYAKNIYEVSADFYLKHGIKTLLVDLDNTLASYRELE
                     PSDTTINFVRNLEKAGIKVLLASNNSGKRVKRYGELLGVPYISGARKPFAFKLRKFLK
                     EQGAELSSTLMVGDQILTDVFCGNTLGVKIMLTDKLVEEDQWTTRINRLLDRPLRNKL
                     RAKGKLKEWNEDDRELKEN"
     gene            4058..4327
                     /locus_tag="PK460_00595"
     CDS             4058..4327
                     /locus_tag="PK460_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53635.1"
                     /translation="MNLSKLKPYRNMLFLALAAGVGAFMPVIGIIVTVVMYVKRDENS
                     LNFTKEERFLLNALLIILIIYLTLNVLYTLKYPEVKPDTSSETSL"
     gene            complement(4324..6912)
                     /gene="parC"
                     /locus_tag="PK460_00600"
     CDS             complement(4324..6912)
                     /gene="parC"
                     /locus_tag="PK460_00600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOE53636.1"
                     /translation="MSKRKPKKQTEEQVITETIIELPMDEIMGDRFGNYSKYVIQNRA
                     IPDARDGLKPVQRRIIYAMHDEGNTFNKPMKKCAHTVGAVMGKYHPHGDTSIYDALAR
                     MSQEWSMRYPLIDFQGNNGSIDGDEPAAYRYTESRLNELSNELVLDINKKTVNMNLTF
                     DDTSFEPEVLPSRFPNLLVNGSQGIAVGVATEIPPHNLREVVDAIIHRITYKRSTIED
                     MRKYILGPDFPTGGVIYKGEGLDSIYETGRGRVEMEALATIESDRTSQRIVITEVPYQ
                     SNKSQILRELGAIEQNRNVDGVVEVRDESDRNGIRIVIELKKEANTDVILEYIFSKTR
                     LRAGYNANIVAIADNRPKTLNILDLIDAYISHQVDVITRRSEFDLATNEKRLHIIEGL
                     IKAISVVNEIIAIIRKSTDKANAKTNIKAAYGFSEEQAEAIVMLQLYKLSNTDITVLM
                     EEKAAIETTIDELRNILSNEKALNNVIIRDLKRIAQKFGDDRRTKIREKGEVVQIDKR
                     DLIADEQVMLALTRDGYIKRSSLKSYRSSGDFALPGVKSSDLLIAASEVNTKDFLVAF
                     TNLGNYLYIPIYEIGEGRWKDEGKHINYIVQLNANEKIIKAFNVKEFREDLYFAIATR
                     NGMIKRTSLNEFVAVRYSRPLQAMRLLSGDRVADVTLTTGNSELLVITESGAASFYNE
                     NEITVVSVKASGVKSINKKKADKVVALISVDPEVRTRTCIVTSEGHLRLLDHSYFEAT
                     ARLGRLQHVMRTFKSDPHTIVSAFRYNRNIEELRVYALTDANVILPLLLTDLAPTPVD
                     KYAKRNINELAEEEKIVSIFNEEIPTISEETKAYAPIVKAEAVKVEPVEDSYEQISIF
                     DLLDED"
     gene            complement(6914..8881)
                     /locus_tag="PK460_00605"
     CDS             complement(6914..8881)
                     /locus_tag="PK460_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOE53637.1"
                     /translation="MADKNITNSYTADSIQVLEGLDAVRKRPGMYIGSTNIVGFHQLV
                     WEVVNNSTDEAMSGYGDKITVTIHKNGSITIADEGRGIPTGINKQTGQPAVHLVFSTL
                     HAGGKFSESTYKTSAGLHGVGASVTNALSEWLDVTVYREGKVHHLRYENGGKLVTPLE
                     VVGNTNRTGTIVNFKPDPKIFGNLEFKWDTITSHLQESAFLLNKVAFIVKDERSGLSE
                     EFYYEKGLIEYVDIINRNKTALSKPIYFEDIYTNNDKLSQPMEIEVALQYCSDDYGET
                     VLSYVNNLRTHDGGTHETGFRTGLTRAVNDFATEHNLLRKGVKLDGNDIREGLTAIIS
                     VRIPESILQFESQTKGKLGTNEANWALNSFIYTKLTYYLQENHEFAVSLIKKCVDAQN
                     ARIAARKAKENLRKTKKVTNNDMILSGKLTPAQSKDYKKNELFIVEGNSAGGTAKSGR
                     DRMYQAVLPLRGKPLNTDTSTVDKMLKNEEFATLISTIGAGFGQNFDIEDAHYGKIII
                     MTDADYDGSHIQTLLLTFFYHYMKPLLKAGMIYIAVPPLYRVSKQVNKKLIFEYAWDD
                     QSLEAAKEKIGPGYKITRYKGLGEMNADQLWETTLNPETRTLIRVNINDPLLVEMRVS
                     TLMGNDANVRRKWIEENVDFNAYDTFIDEVK"
     gene            8986..9669
                     /gene="plsY"
                     /locus_tag="PK460_00610"
     CDS             8986..9669
                     /gene="plsY"
                     /locus_tag="PK460_00610"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOE53638.1"
                     /translation="MHPFIKFLIFLGFGLFGYLIGSINNAVIISNLFFKRDVRNYGSK
                     NAGGTNTGRVFGRKAGITVMVLDILKSVFVYWAITLVFHFTNVGLYVDHAATLYFAML
                     LVALGHCYPVYYKFKGGKAVSVVAGFILATNWVFTILGALIFFLILKLRKMVSYASIL
                     MAAIIVALTPILFIPVLGRISFYPLVVDNLYYYIPTLVGLCILLILKHRTNIERLQKG
                     TESKITWIK"
     gene            complement(9739..9951)
                     /locus_tag="PK460_00615"
     CDS             complement(9739..9951)
                     /locus_tag="PK460_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HOE53639.1"
                     /translation="MPIGAAIGLAVFALILGAVLGYLISRKVFEKQLKKNPPITEKQI
                     RTMLTMMGRKPSEKQVREIMRGMQQS"
     gene            complement(9951..10508)
                     /gene="efp"
                     /locus_tag="PK460_00620"
     CDS             complement(9951..10508)
                     /gene="efp"
                     /locus_tag="PK460_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOE53640.1"
                     /translation="MINVTELRPGNYFTEAGEIYNVLDILLNKTAMRKMIAKLKVKNV
                     RTGAITEQTYNSGYQIDRISISKHKMTYLYDSGDFLVFMNTETFEQVELSKESLTWEL
                     NFIVPNSEVEMAGYEGEIFGITLAPKVALKITECAPGVKGNTVQNATKDAIVETGLRV
                     SVPLFIEEGEVIMVKTTDGSYDGRA"
     gene            complement(10542..10691)
                     /gene="rpmG"
                     /locus_tag="PK460_00625"
     CDS             complement(10542..10691)
                     /gene="rpmG"
                     /locus_tag="PK460_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOE53641.1"
                     /translation="MRVNIQLRCTECNEENYLSSKNRKNNPERIEKMKFCPRCNKQTL
                     HREKR"
     gene            complement(10769..11662)
                     /locus_tag="PK460_00630"
     CDS             complement(10769..11662)
                     /locus_tag="PK460_00630"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOE53642.1"
                     /translation="MSDKLILGSHVGMSAPDYYIGAINEALSYGANTFMIYTGAPQNT
                     RRLATEQMHALEGIKYLKEKGMDIKHIVAHAPYIINLGNSVKPETYELAVSFLQDELQ
                     RVADFGITMVVLHPGSHVGAGVEAGTEQIIKGLNTVLDNDTSGTIIALETMAGKGTEI
                     GRTFEEIRTIIDGVHKKSQLGVCLDTCHINDAGYDVHDIDGVLDEFDRVIGLEYLKVV
                     HVNDSKNERGAAKDRHENIGFGTIGYDTLMKYIYSPRLAHLPKILETPYINGVAPYGH
                     EITMIKNKVWNTTLKDELGEK"
     gene            complement(11659..13014)
                     /locus_tag="PK460_00635"
     CDS             complement(11659..13014)
                     /locus_tag="PK460_00635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE53643.1"
                     /translation="MEFGKYNIDKRLVDTLKMHGYVTMTGVQSEILPRALKGESLIVK
                     AKTGSGKTHSYLIPILNKLEKTEELQALILAPTRELARQIYDFARVLNDDYKGVDILF
                     LAGGLQTSRHKERLASKPALIIATPGRLKALLINEDITSFKSIHTVVLDEGDMLLDSG
                     FYDVISETLAALDNPTVQLFSATIPQKLANLVTLKTGVKTVIDIDRDDKTSDNVTHHL
                     IDVHHMNRFEVVESFVKHFNPYLLLVFCSTNKEVLELYEFLSAKGHKIGLLTGELESR
                     KRKAMFRRVRNNEFQVVVASDIAARGIDIEHVSDVLSVSFPFDLEFYFHRAGRTGRLD
                     NKGNAYTLYDHDDLETVEKVEKLGVKFTRLVYKGGAFSAVTKRISRKKALTPEEKELN
                     VKIKTAVAETKTKKVRPGYKKKVRTAVENVKRKHRRKMIKKSIREQRVERYKAEKRRD
                     S"
     gene            complement(13079..13852)
                     /locus_tag="PK460_00640"
     CDS             complement(13079..13852)
                     /locus_tag="PK460_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOE53644.1"
                     /translation="MKTRTLLTKLGRLYPKRIALKHNDYVGYMAGQKVEEVKRIVLLL
                     DLDEIVLEEALSHKPDLILTHHPFIRGTRAKVFARDEKKKAFALWLDTLPVTVYSMHT
                     NFDEAPNGMNDALAEKLELSAVKPLASEPMARGGYLPYPMTVEEAANYIVDKLEATYG
                     LLVAGDKEREIKRVAIIGGGGSRDWPAAMAEGYDMYISGDAPHYVRRDVMNAGYNYLD
                     VPHEIENAFLWKMKEVLLTIDPSFEVIAIKHEKPPVVIK"
     gene            complement(13849..14517)
                     /locus_tag="PK460_00645"
     CDS             complement(13849..14517)
                     /locus_tag="PK460_00645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE53645.1"
                     /translation="MLSLRLQAIANLIKKDDVVADIGSDHGLLVNYLAIKGHRALYAS
                     ENKKGPYNRLKSELKAYKSDIIEIDLADGLENLPARINTVVIAGMGGELIVSILQAHP
                     AKLVNVQKLILAPHCNAKAVREVLNALNYSISYEEIVVEKEVFYEIIVAEQKAIATKE
                     VNPYFGTYHLQARSENFIKMWTSVYNQNKRILSNKKLPLSRKEELLKEQELIEKVISE
                     GEKV"
     gene            complement(14517..15968)
                     /locus_tag="PK460_00650"
     CDS             complement(14517..15968)
                     /locus_tag="PK460_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE53646.1"
                     /translation="MDDKKLKDLAELEEVAIEEDDEVEIELTLPQIRKFLLEEARRNN
                     NELDQDDVMDATSHLDLSENEFDKLIQYFKNNKVTIHSDIESDEYDPDFANFVDGDDD
                     LDDDLDLDDDFDEEELSFGDAFKDVKEENFEENQRLRKISLGEVKTTDSVKIYMKDMG
                     RYSLLKADEEVTLARRYKEEGDIDARDELITRNLRLVFNEAKKYSSGNMHILDLIQEG
                     NIGLMKAVEKFDYTKGFKFSTYATWWIKQSISRSIADQARTIRIPVHMVETINKISRT
                     QRRLTQEFGRDATPEEISDALIKYSVKDVEEAMELSANATEENPIPEEVANKVRTIMA
                     KVETLTEELKRPATPEEVSESFREFTPARIREIQAIAMEPVSLESPIGEEDDSHLGDF
                     VEDKDNETPTDFVRKSILHDQLRDNMQEFLTPREERVLHLRYGFDDDKPRTLEEVGKI
                     FGVTRERIRQIEAKAIKKLRHPSRTKGLRGHIQ"
     gene            complement(15987..17768)
                     /gene="dnaG"
                     /locus_tag="PK460_00655"
     CDS             complement(15987..17768)
                     /gene="dnaG"
                     /locus_tag="PK460_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOE53647.1"
                     /translation="MENVDIVDVIGRYIPLHKQGQNYSALCPFHDDRNPSLMISPSKQ
                     IFNCFVCNTGGNAIRFVALYEKKTYYEAAQRLADMVSFKHDFFTKKQKFVVRDTKLDP
                     LISAINDLTAYYEYTLITEAGSEAKKYLVNRGLDDALLKKYKIGFAPRDGEATIKFLQ
                     SKGHALKTIEDIGILGGSLERPYDINHGRIIFPLNNKDGQVVAYSARALPSEKKAPKY
                     VNTRETQLFIKSDTLYNYHHAKALMAAQYVYLVEGFLDVIALDRVGERSVVALMGTAF
                     TKEQLALLRLLNKEVRIYLDSDTAGQMATLSVIKDLIAANIPLQIVKPHKDGYDPDDI
                     LNKEGKEALVTALTTFIPRDDFIFNYYLSVTKLDDMASTKKFVNDVMNEIILKLNSRL
                     EVSAMIERLAEASGFNSLVLTDMYESLRRKKQTRNEAINTVKQKLPVRQTLNKVAQAE
                     RFIVYQMLVYPEARTFFDNHVRVFTHEVHRYIVNYLKEFKPKEQVNYALLLNDIQSRF
                     TDEATINEYTQELLTIESGLQDVKYDENILNDAAKTLNYERELQILERKHQDELLRAP
                     DEASYAALKVKHIQAKTELMQKYGKKK"
     gene            complement(17830..19203)
                     /locus_tag="PK460_00660"
     CDS             complement(17830..19203)
                     /locus_tag="PK460_00660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOE53648.1"
                     /translation="MHKHSFEKIVNHLVTSGFVFQGSEIYGGLSNTWDYGPLGTSVKN
                     NISNLWWKKFVTESPYNVGIDAAILMNPKVWHATGHVTTFNDPLIDCRNCKARHRADN
                     LIEDQVKGLNVTGLPNEEMMKIIKEYGVKCTVCGKQDFTDIRQFNMMFKTHIGVTEDS
                     QSEVYLRPETAQGMFVNFKNVQRTMRKKLPFGIAGIGKAFRNEITPGTFTFRTREFTQ
                     MELEFFCKPGTDLEWFKYWKDYCIKFVQDLGIRPEYLRFRDHTKDELSFYSLATTDIE
                     YLFPFGWGEIWGIADRTDYDLKQHMEHSGENFEYLDPDTNERYIPYCVEPSVGLDRLL
                     LMTLVDSYDEEELEKDTRIVMRLAPHLAPYQVAVLPLQRKLSEKALAVFSDLVKDFST
                     VYDETASIGKRYRRQDAIGTPYCVTIDFDTLEDESVTIRERDSMAQIRLPLSELKAYL
                     HSKVKFQ"
     gene            complement(19276..19983)
                     /gene="recO"
                     /locus_tag="PK460_00665"
     CDS             complement(19276..19983)
                     /gene="recO"
                     /locus_tag="PK460_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOE53649.1"
                     /translation="MEVVIIRLTPYREKDYIVNALSKEEGIITFRATGALNVKSKFAG
                     KLFLYALVDVELRESKAGHTLTNVESLTNIAKVFTDEKRIIALNIIGEVISKTLKDND
                     SISEAFPLIKKVLMSLSETTKLISLVYLFTTNLLRILGSALVIDRCVNCGDTKSIIGV
                     SFNKGGLICKKCYDEKTITLNAEEIQMLRYGFMINEEELLRHEFSDEAALSLLTLMLT
                     YLEFTYNYKIMSKDLIK"
     gene            complement(19986..20858)
                     /gene="era"
                     /locus_tag="PK460_00670"
     CDS             complement(19986..20858)
                     /gene="era"
                     /locus_tag="PK460_00670"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOE53650.1"
                     /translation="MIKSGFVSIAGRPNAGKSTLLNTILGEKRAIVTPKPQTTNKNIQ
                     GVYNDLRGQIVFIDTPGIHKPKTPYGEYLNKQAYQSIRGSEVTLLVVDASVEYGAGDE
                     YLFSRLKVDHPLIIVLTKIDLASPIHVQKVKEQWTQNYPDTPLIEVSSVRNFNIDALI
                     TLIFEHLEVGPLYFPLPEDYAIRLENYINEIVREKIIMLTREEIPYSVAVVVDHLTES
                     DTKVEAYLTIYCEKDSQKGILIGKGAKMIKRIRLQTQYELSKFLGKDVLLELMVKVEP
                     LWRSDPKILKKLLG"
     gene            complement(20855..21253)
                     /gene="cdd"
                     /locus_tag="PK460_00675"
     CDS             complement(20855..21253)
                     /gene="cdd"
                     /locus_tag="PK460_00675"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOE53651.1"
                     /translation="MTKEELIKKAREARALAYVPYSKFKVGAALLTKSGKVYVGANVE
                     NASYGLCNCAERNALFHAMMNGEKKEDFVALAVTGKTAGPISPCGACRQVMSELLPKD
                     APIYLTNLEDAFLETTIAELLPFAFDDSDL"
     gene            complement(21250..21717)
                     /gene="ybeY"
                     /locus_tag="PK460_00680"
     CDS             complement(21250..21717)
                     /gene="ybeY"
                     /locus_tag="PK460_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOE53652.1"
                     /translation="MLKLTVEFYDDYAPTYKNYRAIFKRIYAKTMKLLDYMTGEFLVE
                     VTITDEERIRALNKEYRAKDAATDVLSFAFNDIKPGHKFKEPFVHLGSIIICAPVAQR
                     QALELGHDARREFKFLFTHGLLHLLGYDHTNEKEEQVMITLQDQIIGKRGGKK"
     gene            21890..22525
                     /locus_tag="PK460_00685"
     CDS             21890..22525
                     /locus_tag="PK460_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOE53653.1"
                     /translation="MKSDFTFIYSAPKVTDEKLPAVFLFHGYRSDEKDLLSLVASLES
                     THHIFSVRGPLSFMFGGYAFWDMQDLENPNLEQYQSAVESVQNFIVNAITQYNLEKKD
                     ITLLGFSQGAVLVQSIALRAQKLVNKVVALSGHLPYFAKDEEYATLNLNKLHIFISNG
                     INDNVVPYAWGKESADYFKKRGADVTFKSYFSGHYVSRDNARDLIDFIVSN"
     gene            complement(22544..23713)
                     /locus_tag="PK460_00690"
     CDS             complement(22544..23713)
                     /locus_tag="PK460_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HOE53654.1"
                     /translation="MNLIDVRLTQNNTNECGKAVLRMLLAITHRDKRYLKLPLSGDFD
                     NFLALSKKAKEYGVELLGYKLDSIEEVKNVKVPFIMHLTKDDTNHFVLAEFKKGKLSI
                     ADPSGEFYVLSLKNIKKYFISNILVVNCVEKVENPPLVKFKQRYGALIFHSLFLIFLL
                     IGFAFLGHASLDLISYVSFTLAAILKIIEQQVIMKNLQKFDDLYIAPRLVNIKDNFKY
                     KFKVLQEAKTVVFSRPLQLFSAISSTLLITIILVLNNYYFVIISVLLMIVALIEVKRG
                     IKGSAKTFELEVKLSSLAKENNEKRMQLYKEIINDSTHIAAWRVYRSIIIHFSLGVLI
                     FVLMYFTAVYSLNFLIFYFFGFSYYLYELKKLFSLALNTHHYYSAINAINDIPVN"
     gene            complement(23795..23974)
                     /gene="rpsU"
                     /locus_tag="PK460_00695"
     CDS             complement(23795..23974)
                     /gene="rpsU"
                     /locus_tag="PK460_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOE53655.1"
                     /translation="MPKTVVRENETLDEALRRFKRQVNKAGVLSETRKRQYYVKPGLR
                     RKLKAEQARRKARRG"
     gene            complement(24201..24611)
                     /locus_tag="PK460_00700"
     CDS             complement(24201..24611)
                     /locus_tag="PK460_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53656.1"
                     /translation="MLFALLDIFLWRTKFFFIILIPISIFLILFAIKIIILKESSIFV
                     SGDLLVGLLKVEYKYNIDLKDVSFVEVQQRDYKFSSRSREKRGRGIETYRTGHKFIEF
                     NYLDGSVKRIHCNDLSRKQINKILEHCKNKKNYY"
     gene            complement(24696..25292)
                     /locus_tag="PK460_00705"
     CDS             complement(24696..25292)
                     /locus_tag="PK460_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53657.1"
                     /translation="MSNKKDYEDIIKGIGFMKKYKRNIMIKVAGVISELIITFIPIYL
                     ISSLLLFPPLIFKDFIKFTGLTILVIVIVNIIFYIIAAIAKIFIKTAYIITSDSLITQ
                     INNEVHSTIYLKDVYYLTYNFGAIDGQPSPTELVLFDKEHKYLISIENPPLLMVINIK
                     KKCLNAKITYFNSRRILFFLVSSLVISIIVSLISLIAN"
     gene            complement(25473..25751)
                     /locus_tag="PK460_00710"
     CDS             complement(25473..25751)
                     /locus_tag="PK460_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53658.1"
                     /translation="MKKRIIAFYDVLIVAIISTPFTICSIFFFVFADTSNLEWLCKNW
                     YLIIFASLGVSLPVVGIFWITGYTLIMNNHYVFFYYFPHAKTWKKNDK"
     gene            complement(26324..26479)
                     /locus_tag="PK460_00715"
     CDS             complement(26324..26479)
                     /locus_tag="PK460_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53659.1"
                     /translation="MKIYTINDLKLKYKYSEKNIKKRHYKLLLNLLKSVNSGDYDINR
                     YYSLIRN"
     gene            complement(26604..27005)
                     /locus_tag="PK460_00720"
     CDS             complement(26604..27005)
                     /locus_tag="PK460_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53660.1"
                     /translation="MSDTAEFVKVEVIDEENKEGMSRRKKFEIRKLVMSTYVTLVVIA
                     YLAMVFFLDKWHPHWVIFLSIPVVDSLVTAILEKKIALFSVDALIILAFVVVGAVTGK
                     WHPAWMILLLVPLWRYILNTIKRIKRIKAMD"
     gene            complement(27024..27464)
                     /locus_tag="PK460_00725"
     CDS             complement(27024..27464)
                     /locus_tag="PK460_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53661.1"
                     /translation="MQFNQDFDKIIGEDIYLSITEKNSGLIGGLPYYKYGIHLNDGTL
                     VGNILVRIGHNFNSYYSGNFVYQVSGAYRGQNLAVKALNAILVVAKYHKMEYVIFSAP
                     SSSKGYLRTFEKMGAKLLEIVTPPENYMYYYKGIEPLAIYRLDL"
     gene            complement(27502..27987)
                     /locus_tag="PK460_00730"
     CDS             complement(27502..27987)
                     /locus_tag="PK460_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53662.1"
                     /translation="MITFEKSKRYLFYYVGVLLVTITLFISWVVMIVKNPGSTSGDIV
                     LLSFLFLILFGGEALFLIIYLIRPKVVLSVDEEGAYLHLSKKKTKEVAFSNFSSVGNM
                     RRNLVIATKDSELYVVRFLKDHKKAEETLKTMFNTFITNHPERYFDMTESADKQKHEE
                     K"
     gene            complement(28002..28664)
                     /gene="deoC"
                     /locus_tag="PK460_00735"
     CDS             complement(28002..28664)
                     /gene="deoC"
                     /locus_tag="PK460_00735"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006267712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOE53663.1"
                     /translation="MKKNKLIDHTLLKADASPAQIEKLCQEALEYDFMSVCVNPGYVP
                     LASMLLAESDVRVCTVVGFPLGATSTEAKVLEAQIAVEEGADEVDMVINVGKLKAGLD
                     LEVFTEIRAIREALPDWVILKVIIETCLLTDDEKVRACLLSRKAGADFVKTSTGFSTG
                     GATVADVKLMRDTVGSDMGVKASGGVRTMEDLEAMVKAGANRIGTSNGVAFFTGKKGT
                     GY"
     gene            complement(28884..29087)
                     /gene="rpmF"
                     /locus_tag="PK460_00740"
     CDS             complement(28884..29087)
                     /gene="rpmF"
                     /locus_tag="PK460_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOE53664.1"
                     /translation="MAVQQRRGSKTRKRLRRTHFKLNVTGLTTCPNCGTLIRAHHVCP
                     ECHHYAGKDVITKEEPKKGKAKK"
     gene            complement(29226..30512)
                     /gene="mtaB"
                     /locus_tag="PK460_00745"
     CDS             complement(29226..30512)
                     /gene="mtaB"
                     /locus_tag="PK460_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOE53665.1"
                     /translation="MKTFTSVALGCKVNAYEVFSIEETLKAQGYKLANQGDKVDVAII
                     NTCSVTSNADQKSRQHIRKLAKNYPDAILVVMGCYAQLNKDLINTIPEIDILVGTSNR
                     SKIVDYIEEFKEHGQKIIAVEEEPRTFNYEELGATSVTPQARAYLKIGDGCDAFCTYC
                     IIPLARGKMRSRDVRDIVKEAKDLVRRGYKEIVLTGIHTGAYGKDLGNIKFSNIVEMI
                     LEACPSLHKLRVSSIEANEIDDDFLRLMLKDKRIARHLHIPLQSGSDEVLRRMNRHYT
                     TSEFIHKIENIREKVPNIAITTDVIVGFPGESEKLFNETVAFIKKVNFTQLHVFPYSA
                     RTRTPAASFKEQVLGPVKKERVRTLINLSEDLYRSYAQRFVGEKMQVLIETYDDKEEV
                     YVGHTTNYLAVRVKSDSDIKHEVITIIYDPNISKKI"
     gene            complement(30509..31216)
                     /locus_tag="PK460_00750"
     CDS             complement(30509..31216)
                     /locus_tag="PK460_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53666.1"
                     /translation="MKIYELLQENRRGHLITPSREYLFFQDHEALLTQVPELIPFINS
                     KDDFDLICANIIQSALLNYEALTNYWNNEVKPDTELPVKALFTYNNVPIYCPLFSVSN
                     NILIGNNLGSKLNLTHDAIDLFEAYNFSLFESQLTSLVLVGEDIHTRAYYHYDFHAIY
                     IINDQGRLDLKICLFDKHIKRPDFRNVLERIKPALTEYYAGNRIAFINALVKEKLISH
                     KLYTKYINNLKRRKIIG"
     gene            complement(31203..31928)
                     /locus_tag="PK460_00755"
     CDS             complement(31203..31928)
                     /locus_tag="PK460_00755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOE53667.1"
                     /translation="MSYRFFGRVRNNEVVLSDDDIFHLTKVLRLKKDETFEVVAGQSL
                     YLCTYESVNPFILNVLEKRKLPLEPLPRLTLGYALPKGDKLELVIQKAVEIGVSHIIL
                     IDSERSIGKIPATRLESRLARFNKIIKSAAMQANRNYLPTLTEPLAFKEVLDLEFDTK
                     LIAHETANLPLKAVLQQDLGDNLLILVGPEGGFSEAEVTLASTKGFNVITFGDNILRS
                     ETAAIYALSVLHHYQKGAHHENL"
     gene            complement(31956..32771)
                     /locus_tag="PK460_00760"
     CDS             complement(31956..32771)
                     /locus_tag="PK460_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOE53668.1"
                     /translation="MEILKYIILGIIQGVTEIFPISSSGHLVIFQELLKLEQPGLVFE
                     MFTNTASFLALFVLFAKDIWELIKGFFLYIFKKEAREEYKDTFFYVLKLLVAVIPIGV
                     VGLIFKDEMGMIKNLLTVGIALFITGALLLTIFFTRNLIEEHDDITWKDAIVVGFTQA
                     FAVFPGISRSGSTIIGGRASRVSLKSILRFSFLAYIIISVPTSVLGAVDLVHISEEIN
                     WTGYILAFIFTFGATFLTGLFVMKKLKVEHLIYFGIYCLVVGILAFSAFFIMR"
     gene            complement(32887..34518)
                     /locus_tag="PK460_00765"
     CDS             complement(32887..34518)
                     /locus_tag="PK460_00765"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOE53669.1"
                     /translation="MLSKATLAFFALSALLISAISLGSNHGSSARGEPTPYAIVLGTE
                     KNKIAEGSENPDGYTGTGVLTTELGNNIAVSYKHLTNPSTHWQRIKTGGYIVNSDPIN
                     GITNLELTKRDDTVNVEILYSNTMSFDASRKVVINASSPTNFSTDFNGYKPKYIGIYA
                     LADSSFNLGKIEFDCVDHYHTLSVLSKNDARGTVTSEGLFRAGTNVTVTATALAPYFF
                     AGWFDASNNLVSSSATYTFAMPSNNLVLSADFVAEGEEMKFGAYPQTRVTDVTLITTL
                     NNLAGGSEGLPTAENAKAWTDYLYYYNGAVTSFMWYIDLVNEGEEYRGVYYTEYRYTY
                     TDSTAGCFNQQDHGYTKRIVYWFKYEPITWKVLDIDAGKAFLLAQTVLDAQDYFYAKT
                     RRNIDGETVYANNYEHSHIRSWLNSSFLNAAFTTTEQNKIAVTTVDNSKATTINNVHN
                     FDCPNTLDKVFLLSYLDLNGHYGFVDDASRVRYPSDYALSQTVSKDYDKAYWWTRSPY
                     SSETSASRVNYNGALAAINVTDAAMGILPAIWINA"
     gene            complement(34797..35909)
                     /gene="dnaJ"
                     /locus_tag="PK460_00770"
     CDS             complement(34797..35909)
                     /gene="dnaJ"
                     /locus_tag="PK460_00770"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOE53670.1"
                     /translation="MAKRDYYEVLGIKKSASKDEIKSAYRKLAKKYHPDVNKEKDAEE
                     KFKEVQEAYDVLYDDQKRATYDQFGHAAFDQNGGNPFGGGFSGSGFGFDFGDIFEGFF
                     GGGRRASRQTGPARGNDLLTRVRINFMDAINGTKIEFMHTYDKMCETCQGKGAENAND
                     IHTCSKCNGSGVETFVQESIFGRVQSQRACTSCGGSGKTISKPCKSCNGAGYTRVKEK
                     QLLNIPAGIGEGQKLRIQGKGERGLNGGPNGDLHVEIVIKPHNSFKRVGNDIYLQIPI
                     SFVDAALGVTLEVPTVYGNVEVKIPAGTQPGATLRLSGQGVKDVRGNVGNQYLEVEVK
                     TPTNLSKDQKVALEDFKKAEPKKETFFEKFKSKFKR"
     gene            complement(35977..37797)
                     /gene="dnaK"
                     /locus_tag="PK460_00775"
     CDS             complement(35977..37797)
                     /gene="dnaK"
                     /locus_tag="PK460_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOE53671.1"
                     /translation="MAKKEIIIGIDLGTTNSVVSYRQADGQIKVIPTPEGKPTTPSVV
                     AFKPDGEQVNGEAAKRQAVTNPDTVMSVKRHIGTNKKFKINALKKEFTPEEISARILG
                     YMKDYAEKNLGQSIQKAVITVPAYFNDAQRQATKVAGEIAGLEVVRIINEPTAAALAY
                     GLDKEHDEKILVFDLGGGTFDVSILEIGDGTFEVIATAGDNHLGGDDWDAEIVEWIKQ
                     EIEREHGLKLNDKMAMQRFREAAEKAKIDLSQMLETTISLPFIGMGASGPISYEGKLT
                     RAKFLDLTKHLLARVEEPIRRALSDAKVKASDLNQVILIGGSTRMPAVQELVKKMTGK
                     APNLTVNPDEAVSIGAAIQGAVIAGDVKDVVLLDVTPLTLGIETMGEVMTPLITRNTT
                     IPTTKSQIFSTAADNQPSVDIVVYQGERPMARDNKLLGHFRLDGIKPARRGQPRIEVT
                     FDIDVNGIVKVTAKDLDTQKEQHITITNSSGLSKEEIDRMVKDAEEHKEADEKRKKNV
                     ELKNRAEQYINEIEHSLEEGGDKVDANQKEASEKLVTELKEAIEKEDYETLEKRLSEL
                     EQMAQMMQNMQQGQAEGETSATETPKDDDIIDADFTDKKD"
     gene            complement(37822..38412)
                     /locus_tag="PK460_00780"
     CDS             complement(37822..38412)
                     /locus_tag="PK460_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOE53672.1"
                     /translation="MSDEKKHVEKDVAPKKRHLAKIQKLEAEIEKLKAESAEWHNKYM
                     RVLADMDNARKQNAKDQQYYIKYRAVPFIEKLLPALDIFNHVIREEQEDPVLNNYLTG
                     FRFVYGQLLDALTSEGLEEVVVNVGDKFDENSMHAVEVKYSEEVEPNTVLGVRNNTFK
                     FIDRLIRPAQVIVSTNIKEEPKNDDNVPEVNPEDLN"
     gene            complement(38405..39421)
                     /gene="hrcA"
                     /locus_tag="PK460_00785"
     CDS             complement(38405..39421)
                     /gene="hrcA"
                     /locus_tag="PK460_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOE53673.1"
                     /translation="MRYRRRELILKYTVELFIKTAQPVGSKSLIDNYNLNYSSATVRA
                     EMNALEGMGFLEKPHASAGRVPSKKGYEFYVANLREDEVNDNFRHHIATLVEEKTKSV
                     EQVINQTCEILSQMTNLVSVALGPEKKGETLQSIQVVALDKNMGTAIFVTNRGYVEHN
                     TFIVPPSTSIQEISKVANIFNERLVGTRISEVSAKMELLQPLLTDYIVEQEVLANAFT
                     DAFIRYARDRLSLYGKESLLEQPEFINDSEKLRKLISILDDPEKIRELITGNGDLSVN
                     IGGKDEDLTFISANVHVPGDQVGQIVLVGPKRMDYSKVLWALEYVTDELEKYFEIERE
                     INDE"
     gene            complement(39546..41537)
                     /locus_tag="PK460_00790"
     CDS             complement(39546..41537)
                     /locus_tag="PK460_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOE53674.1"
                     /translation="MIQDNKQSKFQKIFHEFGLYAYFIGLLLFLFALLAFIPGVSFLS
                     DKAVMILNLIALILAGYKVISEGLVETVKESIKSKRFRPNIHILMALGAIGAIIIGDY
                     GDAALLILIFAGAYFLEEYAEAKSAKEITNLLKITPTTARLLLQDGTQQEVSVESLKV
                     GDKVLVLNGDQIPTDGTIVSGSAAINEAMITGESMPKEKHEGDEVFGGSINGNSTFTM
                     VVTKNSDETVLAKILRVVSETQTNLTKTATFIKKFEPIYVTIVMLIAPLFYLFGYYVL
                     GWNEVISANGMTLALKRTIILFIGASPCALAVSDIPASLSALSLLARRGVLFKGASSL
                     ALFADVEAVAFDKTGTLTEGKPSVTDVNIPPGVSAEEAEEGKVFLVSMEKKSNHPLAT
                     AIVSHFTPIKTVDLEVEHKIGSGLRAMYDGNEYLVGKPALFNLPERCANRAKELAVQG
                     KTVVFIAKNGKPILLIALLDMIKCCAFDGLEYFRKQEINTVMITGDNELTAKAIGKEL
                     GITRIYANVLPEQKLDIVKELKKEYGGVAMVGDGINDAPALVEATIGIAMGDGTDVAI
                     DVADGVLVNNDFQKLSLMHAISRKLKRIVIQNIVLALSVVLLLIALNFVVTLPMGLTV
                     AIHEGSTILVILNGLRLLNFNEKHAIKKPKRSKFNKKIA"
     gene            complement(41542..41826)
                     /locus_tag="PK460_00795"
     CDS             complement(41542..41826)
                     /locus_tag="PK460_00795"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOE53675.1"
                     /translation="MDKVVDIERLRDFFKVLGDDTRMRIILLLEESAKNVTEIANALN
                     MTQSAISHQLRTLYDHRVVTYSKAGQERIYEILDPHIYEIIKQARDHIRE"
     gene            complement(41906..43000)
                     /gene="hemW"
                     /locus_tag="PK460_00800"
     CDS             complement(41906..43000)
                     /gene="hemW"
                     /locus_tag="PK460_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOE53676.1"
                     /translation="MTKRGLYVHIPFCTSLCHYCDFVKVIYQKKWVASYLEKLKHDLS
                     FFNVSRDLSTLYIGGGTPSSLSYYELRNLLELLKPYTANVKEYTVEANVESLTLPKLK
                     LMRSYGVNRLSIGVQTTDDYMLKKLNRHHTYTMIKTKIALVKKALFTNFSVDLIYGLP
                     GQTLSDLENDLMNIIKLGAPHISTYSLTIEKGTMAALKKWEVPSDDTLREMYDFILKT
                     LRNNNYIRYEVSNFALKGYESIHNKLYWENRNYYGIGLGASGYIDGKRYLISGGLTKY
                     LKGESIINYDEITSEEYVEEYLMLNLRLAQGFSLQDFKTRVGFDFLEKYGTKVKNLIE
                     RGLLNVTDDKVYATDEGIMLLDQVVLTLIM"
     gene            complement(42993..43244)
                     /locus_tag="PK460_00805"
     CDS             complement(42993..43244)
                     /locus_tag="PK460_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53677.1"
                     /translation="MIYLPTLAIDSPGLILLFLLLFFGLIALITFIIRRLIPGLKDKG
                     GRVNEQVAVQQELDRVLEPITDEKILAEFEKVDKETKND"
     gene            complement(43255..44736)
                     /locus_tag="PK460_00810"
     CDS             complement(43255..44736)
                     /locus_tag="PK460_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53678.1"
                     /translation="MSTNQGERFSNERYLTMNQVSRALGTTLIDDIWKEVLQYRNMFS
                     VTIPLRTVERNRLRVVLTPKINDRLNQLDRRISRIQARIQEVYVDANHQKALKTLLTR
                     DILKTIAASYEINFHDGIVNQLINHNISVLNAWEMFLNDYYRGLELVAKSANLDFTEA
                     LLADVGRLFNVPPLSNYIYRTTEIKTGRQVSVINHVHPHVPVNQISDAIADFYAFMAT
                     STLPTIVQSAAIIFYLDYIKPFEAHSEIIAILMAKAFLAERELALIAPYLNFELILER
                     YRDARDDVFAEVKKSADLTYFIDLLINVLNESLQTIEERIVFVVTHELEAEKHVLPPF
                     EEETQEESVSATPPTDLFEHAQAEVAEKEETFKSEPVPVYTPKKSAIPLPLPPREEGV
                     KVTPEEANLGITQLPPELSENDILRLERHIRESDPSLSRAQAYFYARHCTMGKYYSIN
                     DFKEVTGCAYETARTSMEHLANSGYYRKEKFKNKFIYTPTRKA"
     gene            complement(44815..46626)
                     /gene="lepA"
                     /locus_tag="PK460_00815"
     CDS             complement(44815..46626)
                     /gene="lepA"
                     /locus_tag="PK460_00815"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOE53679.1"
                     /translation="MNYNKDLIRNFSIIAHIDHGKSTLADRLIETTGTLEKREMKAQV
                     LDSLELERERGITIKLNAVALDYLAKDGKTYRLNLIDTPGHVDFTYEVSRSLAACEGA
                     ILVVDATQGVEAQTLANMYLAIDNDLEIIPVINKVDLPSARVEEVREEIHKRIGLDLS
                     KVIGVSAKTGFNIEEVLEAIVKYVPAPSGNVKAPLKALIFDSYYDPYRGVIIMMRVKE
                     GEVRVGDEVLMMATNRKYLVTELGIRTPQEVKRDVLRAGEVGYLAAQIKDIKDISTGD
                     TVTLVNNKAKEALPGYREITPMVYCGLYPVDSKDYGDLKDALEKLSLNDAALKYEPET
                     SVALGFGFRCGFLGLLHMDVIQERLEREHDLNLILTAPSVIYHVYLTNGTMLKIDNPS
                     KLPDFTLVDRIEEPYVKAQIMTPQEYVGPIMELCQDKRGIYVDLSYLDDTRMQVTYEI
                     PLSEVIYNFFDKLKSYSKGYATLDYSLSGYKASNLAKMDILLNGQPVDALSLIVYRPT
                     AYNRGVAMCRALKNIIPRQQFEVPIQAAINNKIIARTDIKAVRKDVLAKCYGGDISRK
                     RKLLEKQKEGKKRMKLVGSVEIPQEAFMSVLSIDDDN"
BASE COUNT        13933 a         9333 c         7515 g        15963 t
ORIGIN      
        1 aagtagtgac tatcaagtta ttaaagtgtc tacatttagt tgactagtgc acttttgtga
       61 taaaacgata aattcaattg tatttataat cataatatcg aaagtgagga ttttttatgt
      121 acgaaaatga acgtttcctg cgcattagcg acaccgcaag tggttttacc gaccacagac
      181 tcaaaaagca atatttcgcg ctctttgatt ggtataaacg caatttagag tatgaaatgt
      241 ttctcgcaaa taataaactc cgctataaga ttaatcaagg ggaggtttat gagattgatt
      301 ttggccgtaa tgttggaagc gaacttaacg aaagacatta cgctgtcgta ttacatcata
      361 gtgatgttga ggcacaaaat attgtcgtcg ttcctttaac aactaagatt cattttagtt
      421 atggggaagc aattgatcta ggatatttac cagaagtaaa aacgacagaa aaaagctacg
      481 caaagattag ccaaattaga acggtcgata aagcgcgcat ttaccttcga cctattattc
      541 atactgaaga taataagccg tgcagacata cttacggccc ggtaacgaaa ttaacagcag
      601 accaatttag attagtgatt gaaggactga ataaattgct caataatcaa ttgtagtaat
      661 taattagtag ttatttagta tgtgtgaaat tgtggataac tctttgcttt gcgcaaagtt
      721 taattaaata aataatcaaa taatttttaa cttaataatc gtataatcac gctattttac
      781 atatttagtt tacataataa accttattga aagtggtata tttttcgatt ttataaccaa
      841 aaataaaaaa acaggctcat tggcctgctc ttcatcaatt taatattagt aggttatttt
      901 aaatactttt taaagcatgc attagttaac gcgttatcaa tatctttgcc cttacttatt
      961 aaataatctt tgtttgcctg taagacaatc acaaagcctt tattaatatc cttaacacat
     1021 tcagcaatat actgactact atcaaaaccg cgggaaggtt caattttatc agcagcgtaa
     1081 attacttttg ctaacttgga catcttctta tgccctgttg cgtgatactt aacagcgtcg
     1141 attaatttct tatctttctc taagtattct tctttcatta aataagcacc aacgaactga
     1201 tgatgtgacc agagtggcat attaagatac tttgggaaat gttcttccat aatttttaaa
     1261 ctttttgctg ctggtacttc tttacctata tcatggaata accctgcttt aaatgctttt
     1321 aagggatcaa catcattagc gatagcaatt aagcgactaa gactagcgac tgaaagcgaa
     1381 tgataatagc gttcatcact catccgttta gcgactaggc cactaaagta aagtttattg
     1441 ttgccaatat actctaatac aacacttggg acatcaatac tttcaaaatt acgaaccttt
     1501 gtactacttg tgctacttaa gggaccagta atttcaagca tatcaaagcg ttcaaagttt
     1561 tcatttgata ctttagaacc tttgcgttta taaacgagaa ttttagcttg ttcggcgatg
     1621 agttcaggat gtttccactg gtcaaaagcc attgcttggt cttcaccaag cagtaaatat
     1681 agttcatcgt gcggataatg ctcacgtagc cgcataaatg tgaggtaagt ataatttaat
     1741 ttaccttcat tcccttcttc aatacgcgaa agttcaaacc cttcttcatc ttttaaagcg
     1801 gcactgatca tgtttgcgcg atgattaaag ctcgtttgca cctttttcca cactgttgaa
     1861 ggtgaaagta aaaataacac aaggtcggca ccaatttcgc gttttgcgtg acgggcaatt
     1921 aagagatgac cattgtgcaa gggatcaaaa ctaccaggat atacaattat tttcataacg
     1981 ggaatttaat tttcggcgct ttaacatttt gccgatataa agtaaatgtt ttaccttttg
     2041 tttcaacaac agtagcgttc aggcgtgccg ctacttctag cgctagactt gttacttctt
     2101 tatcaacgct ttgtaagaca tggactttaa ttatttcttt agcgacaagt gctttttcta
     2161 aaagacttaa gaaactatca gtaatttcat ttttaccaag ttgatattgc gccttaagtt
     2221 tattacttag gccacgtaaa tagcgcattt ctttcttcgt taacatataa ttaaatctta
     2281 ctttcgttat aacttatggc aacaccacgc ggagcgtaaa tgcggacgac acggccacta
     2341 cctttaaaac taacaaagcc taagccgttc caactaacat catacttttt attttcatat
     2401 gttgtaaatt caaaggcgtc aagatcttgg acaccggtga caagataaga aatcggtcta
     2461 gtcttttgat gggcaatcat attattaaat gtgcgatcag cgagtttaat atgtgtacgc
     2521 gtaagtttta taccttccgc aaaatagaaa gtaaaaccag taaaaggtcc gtcaacataa
     2581 tcaaaacgcg caatcccacc gattaaaatg ctttgtttag cgagtaattg gtaagttctt
     2641 ggcttaattt ctttacgcgg cacaattacc tttacgactt ctttttcggc aaggcctaag
     2701 agactattat taatcgcaat ccctggtgta tcataaatcg ttgttgtttc actgattggg
     2761 atttgttgga ccgctagtgt ggtattagga taaatacttg tcgtaatata acgactagtt
     2821 tcatttttaa agttccgcag taatgtttta attaaactag atttcccgct gctgacagca
     2881 ccaatgaaat aaacatcacc ttttttccgg aaattagcca tttcttcgag tacttcatca
     2941 atattatagt ttcttgtact actagcgacg ataatctttt taacattaag tccttcgcgt
     3001 tcaactttag tggcgacata gtctttaagc ttagcttcat ttgtcgattt tggtaataag
     3061 tcaaatttat ttgcgactaa aattatatct agacctgaaa gagccgtatt tatttctgga
     3121 ttaaaacttg attcaaagtt aaagacatca acgacatata ccacaagggc atttttggct
     3181 ttaatatctt taattaagtt cataaaatcg agcgtgacaa ctggtgaatg gagtttatca
     3241 gcgctatagt gatgcgtttt aaaacagcgt tgacataaga aaacatcgtc gcgcttaaga
     3301 acattacttg gtacatatcc tatttcttct tcattttcac tttgaagcac gactccgcat
     3361 ccataacact ttttaatttt ctttaagttc tctgtcatct tcgttccact cctttaattt
     3421 gcccttggct cgtaatttat tgcgtagtgg gcgatcaagc aggcgattaa tacgtgtcgt
     3481 ccattgatct tcttccacta atttatctgt caacattatt ttaacaccta aggtgttccc
     3541 gcaaaagaca tccgttaata tttggtctcc aaccatcagc gtgctactta actctgcgcc
     3601 ctgctcttta aggaatttac ggagcttaaa cgcaaatggt ttacgagctc ctgaaatata
     3661 cgggacacca agtaattcac catatcgttt aacgcgttta ccactattat ttgatgctaa
     3721 aagtacttta ataccagctt tttctaagtt gcggacaaaa ttaatagttg tgtcacttgg
     3781 ttctaattca cgataactcg ctagtgtgtt atctaaatca acgagtaagg ttttaatccc
     3841 gtgcttaagg tagaagtcag cacttacttc atatatattt ttagcgtaat aattcggtgt
     3901 aaatttcttc ataatatctg tcacttctaa agtataaggt tattttatca tggacgatat
     3961 aattctcgct taacaacagt aaatcttttt ataaaagatt aaacctttaa atccttcttt
     4021 tcttataaat cgttagcgta tgtgctacaa tttagttatg aatttaagta aattaaaacc
     4081 gtatcgtaat atgctcttct tagcgcttgc cgctggtgtc ggtgctttta tgcccgtcat
     4141 tgggatcatt gttacagttg taatgtatgt aaagcgtgat gaaaactcgc ttaattttac
     4201 aaaagaagag cgttttttac taaatgccct tttaattata ttaataatat atctcacact
     4261 taatgtgctc tatactttaa aatatcccga agttaaacca gatacaagta gtgaaacttc
     4321 actttaatct tcatctaata aatcaaagat actaatttgt tcataagagt cttcaaccgg
     4381 ctctactttt acggcctctg cttttacgat tggggcataa gcttttgttt cttcgctgat
     4441 agtggggatt tcttcattaa agatactaac aatcttttct tcttcggcga gttcattaat
     4501 attgcgtttt gcatatttat caactggcgt tggcgctagg tcagttaaga gcaatggtaa
     4561 aataacattc gcatcagtta aagcatacac tcttaattct tcaatatttc gattataacg
     4621 gaaggcactg acaattgtgt gtggatcact tttaaaagtg cgcattacat gttgtaatct
     4681 tccaaggcga gcggtcgctt caaagtagct atgatcaagt aatcgtaagt gcccttcact
     4741 agtgacaata catgttctag ttctgacttc tgggtcaaca ctaatgagcg cgacgacttt
     4801 atccgctttc tttttattaa tcgatttaac accacttgct ttaacgctta caacggtaat
     4861 ttcgttttca ttatagaaac tagcagcgcc actttcagta ataactaaaa gttcactatt
     4921 tcccgttgtc agtgtgacat cagctactct gtcaccactt aatagccgca tggcttggag
     4981 cgggcgcgaa taacgaacag caacaaattc attaagactt gttcttttaa tcatgccgtt
     5041 acgggttgct attgcaaaat ataaatcttc tctaaattct ttaacattaa acgctttaat
     5101 aattttttca ttagcgttaa gttggacgat gtaattaata tgtttacctt cgtctttcca
     5161 tcttccttct ccaatttcat aaatcggtat ataaagataa ttacctaaat tagtaaacgc
     5221 tacgagaaag tcttttgtat ttacttcact tgcggcaata agtaaatcac tacttttaac
     5281 accaggtaag gcaaagtcac cactactccg atatgatttt aaactactgc gtttaatata
     5341 accgtcacgc gtaagcgcaa gcattacttg ttcgtcggcg attaagtcac gtttatcaat
     5401 ttgcacgact tcaccttttt cacgaatctt cgtacgacgg tcgtccccaa atttttgcgc
     5461 aatccgtttt aaatcacgaa taataacgtt attaagcgct ttttcattac ttaagatatt
     5521 acgcaattca tcaattgttg tttcaatggc cgctttttct tccattaata cggtaatatc
     5581 agtattactt aatttataaa gttggagcat gacaatagct tcagcttgtt cttcactaaa
     5641 gccataggcc gctttaatat tagttttcgc atttgcttta tctgtacttt tacgaataat
     5701 cgcgattatt tcattgacaa cactgattgc tttaataagt ccttcaataa tatgtaaacg
     5761 tttttcattt gtcgctagat caaattcact gcgacgcgta ataacatcaa cttggtgact
     5821 aatatatgca tcaattaaat ctaaaatatt aagcgtcttt ggcctgttat cagcgattgc
     5881 gacaatatta gcgttatagc cagcgcgtaa ccgcgtttta ctaaagatgt attctaaaat
     5941 aacatcagtg ttcgcttctt tttttagttc aataacaatc cgaattccgt ttcggtcact
     6001 ctcatcccgc acctccacaa caccgtcaac attacggttt tgttcaatcg ctccgagttc
     6061 ccgtaaaatt tgacttttgt ttgattgata tgggacttca gtgatgacaa ttctttgact
     6121 agtgcggtca ctttcaatag tggctagtgc ttccatttcc acgcggcccc gtcctgtttc
     6181 ataaatacta tctaagcctt cacctttata aatgacgcca cctgttggga agtctgggcc
     6241 taaaatatat ttacgcatat cttcaattgt tgagcgttta taagtaatgc gatgaataat
     6301 tgcgtctact acttcgcgta agttatgcgg cgggatttcg gtagccaccc caacagcaat
     6361 cccttgtgaa ccgtttacta aaagattcgg aaagcgtgat ggtaatactt ctggttcaaa
     6421 gcttgtatcg tcaaacgtta aattcatatt aacagtcttt ttattaatat ctaacactag
     6481 ttcattagaa agttcgttta agcgtgattc agtgtaacga taggctgctg gttcatcacc
     6541 atcaatacta ccgttattac cttggaaatc aataagcgga tagcgcatgc tccattcttg
     6601 cgacatacgc gcgagcgcat catatatact agtgtcacca tgtgggtgat atttccccat
     6661 caccgccccc acggtatgcg cacacttttt catcggttta ttaaaagtat tgccttcgtc
     6721 atgcattgca taaattatcc gccgttggac gggctttaag ccgtcacgtg catcaggaat
     6781 cgcccgattt tgaatgacgt attttgaata attgccaaaa cggtcaccca taatttcatc
     6841 cattggtaat tcaataattg tttcggtgat tacttgttct tcagtttgtt tttttggttt
     6901 tcgttttgac atcttatttc acctcatcga taaatgtatc atatgcgtta aagtcaacat
     6961 tttcttcaat ccatttgcgt ctaacattcg catcattccc catcaaggta ctaacacgca
     7021 tctctacgag taaaggatcg ttaatattaa cgcgaattaa tgttcttgtt tcgggattaa
     7081 gtgttgtttc ccataattga tcagcgttca tttcccctaa acctttataa cgcgtaattt
     7141 tataaccggg accaattttt tctttcgctg cttctaaact ttggtcatcc cacgcgtatt
     7201 caaagataag ttttttattc acttgtttac tcacacgata taaaggcggc acggcaatat
     7261 aaatcatccc cgcttttaaa agtggtttca tatagtgata gaaaaaggtt aataataatg
     7321 tttgaatatg tgaaccgtca taatcagcgt ccgtcataat aatgattttg ccgtaatggg
     7381 catcttcaat atcaaaattt tggccaaagc cagcaccaat agtgctaatt agtgtcgcaa
     7441 actcttcgtt ttttagcatt ttatcaacag tacttgtatc agtgttaagt ggtttaccac
     7501 gaagtggtaa cactgcttga tacatccggt cacgtccgct tttagcagta ccaccagcac
     7561 tattaccttc aacgataaag agttcatttt tcttataatc tttactttgc gctggtgtaa
     7621 gtttaccgct taaaatcatg tcattattcg taactttttt cgttttgcgc aagttttctt
     7681 ttgctttacg cgcggcgatt ctagcgtttt gcgcatcaac acatttttta attaaactaa
     7741 cggcgaattc atgattttct tgtaagtagt aagtaagctt agtgtaaata aaactattta
     7801 gtgcccagtt tgcttcattt gtgcctaact ttccttttgt ttggctttca aattgtaaaa
     7861 tgctttctgg aatacgtaca ctgataattg ccgtaagtcc ttcacggatg tcgttaccat
     7921 caagtttcac gcctttacgt agtaaattat gttcagttgc aaagtcatta acagcgcgtg
     7981 ttaatcctgt tctaaaccct gtttcatgtg taccgccatc atgcgtgcgt aaattattaa
     8041 cataggagag gacagtttca ccataatcat cgctacaata ttggagcgct acttcaattt
     8101 ccattggttg acttaattta tcattattcg tataaatatc ttcaaagtaa attggtttac
     8161 taagcgcggt tttattacga ttaatgatat caacatactc aattaagcct ttttcataat
     8221 aaaactcttc acttaaacca cttctttcat ctttaacaat aaaagcgact ttattcagta
     8281 agaaggcact ttcttgtaag tgacttgtaa ttgtatccca tttaaattct aaattaccaa
     8341 agattttagg gtcaggttta aagttcacaa ttgtcccggt gcgattagtg ttccccacta
     8401 cttctaaggg agtgactagt ttaccgccat tttcataacg gagatgatgc acttttcctt
     8461 cacgatatac agtcacatca agccattcgc ttaacgcatt tgttacacta gcgccgacac
     8521 cgtggagacc cgcacttgtt ttataagttg attcactaaa tttaccacca gcgtgtaatg
     8581 tggaaaatac gaggtgtacc gctggttgtc cggtttgttt attaatgcct gtggggatcc
     8641 cgcgtccttc gtcagcaatc gtaattgagc cgtttttatg aattgtaaca gtgatcttat
     8701 caccataacc actcattgct tcgtctgtac tattattgac gacttcccag acgagttggt
     8761 gaaaaccaac aatgtttgtt gacccgatat acatcccagg tcttttgcgt accgcatcga
     8821 gtccttctaa aacttgaatc gaatcagcgg tatatgaatt tgtaatattc ttatctgcca
     8881 tttgattttt ccttatcatt tcacaatact ttgttattat aacaaaaaat tactttattt
     8941 tataattgga ctattttaat ttatactatt taagggaggt tacttatgca tccatttata
     9001 aagttcttaa tattcttagg attcggactc tttggctact taattggctc gattaataac
     9061 gctgtcatta ttagtaatct cttttttaaa cgtgatgtca gaaattatgg cagtaagaat
     9121 gcgggcggga caaatactgg tcgtgtcttc ggccgtaaag caggaatcac tgtaatggtg
     9181 cttgatattt taaaaagtgt ttttgtatat tgggcgataa ccctagtctt ccacttcact
     9241 aatgtgggac tttatgttga tcacgcagca acactttact ttgcaatgct tttagtcgcg
     9301 cttggccact gttatccagt gtattataaa tttaaaggcg gtaaagctgt gagtgtcgtc
     9361 gctggtttta ttcttgccac aaactgggta tttacgattt taggggcgct tattttcttt
     9421 ttaattctta aattacgcaa gatggtcagc tatgcatcga tcttaatggc cgctattatc
     9481 gttgcactaa caccaatttt atttatccca gtacttggtc gtattagttt ttatccgctc
     9541 gtcgtcgata atctttatta ttacatcccg acactagtcg gtttatgtat cttattaatt
     9601 ttaaaacacc gcacaaatat tgaacggctc caaaaaggca ctgaatcaaa aattacgtgg
     9661 ataaagtaat tcttgccact aataaataaa agctaggacg tgtgtcctag cgtttttatt
     9721 tacatataat cttaataatt aagattgttg cattccgcgc ataatttcgc ggacttgttt
     9781 ttcacttggt ttacggccca tcatcgttaa cattgtccga atttgttttt ccgtaatcgg
     9841 tggatttttc tttaattgtt tttcaaatac tttacgacta atgagataac ctaataccgc
     9901 acctagaatt agcgcaaaga ctgctaaacc tatcgctgct ccaattggca ttatgctctc
     9961 ccatcgtaac taccgtcagt tgttttgacc ataattactt ctccttcttc aataaataat
    10021 gggacactga ctcttagtcc tgtttccaca atcgcgtcct ttgtcgcgtt ttgtacggtg
    10081 ttacctttaa cacctggcgc acattcggta attttaaggg cgactttagg cgctaaagtt
    10141 atgccaaaaa tttccccttc ataaccggcc atttctactt cactattggg gacaataaag
    10201 ttaagttccc aagttaatga ttctttggat aattcaactt gttcgaaagt ttcagtattc
    10261 ataaacacta agaaatctcc actatcataa agataagtca ttttgtgttt gctaatacta
    10321 atcctgtcaa tttgataacc gctgttgtat gtttgttcgg taatcgcacc agtgcggaca
    10381 tttttaactt taagtttcgc aatcatttta cgcattgctg tcttattaag taagatgtca
    10441 agcacgttat aaatttcacc agcttcggta aaataattac cagggcgtaa ttctgtaaca
    10501 ttaatcataa tttaaccctc ctctaattaa ttttaaatag tctagcgttt ttcgcggtgt
    10561 agcgtttgtt tattgcaacg tgggcaaaat ttcatttttt caatccgttc gggattgttt
    10621 ttcctgtttt tactggaaag ataattttct tcattacatt ccgtacatct taattgaata
    10681 tttacacgca taattcttca tctcctctat ttgcttgtat atcatatcat aaatttagaa
    10741 agtattacta aaagtaatat aattcgtctt atttttcccc taattcatct ttaagcgtag
    10801 tgttccatac tttattttta atcattgtaa tctcatgacc ataaggcgcg accccattaa
    10861 tataaggtgt ttctaagatt ttcgggaggt gtgctaagcg gggactataa atatatttca
    10921 ttaaagtatc atagccaatc gtcccaaagc cgatgttttc atggcggtct ttagcggcgc
    10981 cacgctcatt tttggaatca ttaacatgca cgacttttaa gtattcaaga ccaatcacac
    11041 ggtcaaattc atctaagacg ccatcaatat catggacatc atatcctgca tcattaatat
    11101 gacatgtatc aagacagacg cctagttggc tctttttatg aacgccatca ataattgtgc
    11161 gaatttcttc gaaggtacgg ccaatttccg tgcctttgcc tgccattgtt tctaacgcta
    11221 tgatcgtccc acttgtgtca ttatcaagca cagtattaag gcctttaata atttgttcag
    11281 taccggcttc cacacctgca cccacgtgtg aaccaggatg aagcacaacc attgtaatcc
    11341 caaaatcagc aacgcgttgt aattcatctt gtaaaaaaga taccgctagt tcataagttt
    11401 caggttttac actgttgcct aaattaataa tataaggggc atgagccacg atatgcttaa
    11461 tatccatccc tttttctttt aaatatttaa taccttcaag tgcatgcatt tgctcagtcg
    11521 ctaagcgtct tgtattttgc ggtgcaccag tataaatcat aaatgtattt gcgccatagc
    11581 ttaaagcttc attaattgcg ccaatataat aatcgggagc actcatccca acatgactac
    11641 ctaaaattaa tttatcactc atgaatccct ccttttttcc gctttataac gttcgacacg
    11701 ttgttcacgg attgactttt taatcatctt acggcgatgt tttcttttta cattttccac
    11761 cgctgttcta actttctttt tatagccggg tcgcactttc tttgtttttg tctcggcgac
    11821 cgctgtttta attttaacat taagttcttt ttcttccgga gttagtgctt tcttgcgact
    11881 aatgcgcttg gttacagcac taaaagcgcc acctttatac acaagacgcg taaatttaac
    11941 acctaacttc tctacttttt cgactgtttc taagtcatca tgatcgtaaa gcgtataagc
    12001 attaccttta ttatctaaac gccctgttct acccgcacgg tggaagtaaa attctaaatc
    12061 aaaaggaaaa ctaacgctaa gaacatcact aacatgttca atatcaatcc cgcgcgctgc
    12121 aatatcactc gcaacaacaa cttggaattc attattacgc acccgtctaa acattgcttt
    12181 acgtttacgg ctttcaagct caccagttaa aagtccgatt ttatgacctt ttgcacttaa
    12241 aaactcataa agttcaagca cttctttatt cgtggagcaa aacactagta ataaataagg
    12301 attaaagtgt ttaacaaaac tttcgacaac ttcaaagcga ttcatatggt gtacatcaat
    12361 taagtgatgg gtgacgttat cacttgtttt atcatcgcgg tcaatatcaa tcacggtctt
    12421 tacacctgtc tttaaagtaa caaggttcgc tagcttttgc ggaatagtcg ccgagaaaag
    12481 ctggacagta ggattatcaa gcgctgctaa agtttcggaa ataacatcgt aaaaacctga
    12541 atctaaaagc atatcacctt catcgagcac gacagtatga attgatttaa aacttgtgat
    12601 atcttcgtta attaataaag cttttaatcg gccaggcgtc gcaataatta gcgctggttt
    12661 actcgctaaa cgttctttat gacgactagt ttggagtccg ccggcaagaa acaatatatc
    12721 tactccttta tagtcatcat ttaaaacacg agcaaaatca tagatttgcc gcgctaattc
    12781 gcgggttggt gctaagatca gtgcttgaag ttcttcggtc ttctctaatt tatttaaaat
    12841 tggtattaaa taactatgag ttttcccgct acccgtcttc gctttaacga tgagtgattc
    12901 accttttaaa gcgcgtggca atatttcgct ttgcacaccg gtcattgtta cataaccgtg
    12961 catttttaaa gtgtccacta atcttttatc aatattgtat ttaccaaact ccataatatc
    13021 aattccttat tatctactac ataactacta atttattact tataaaatac ttttaccgtt
    13081 attttataac aactggcggt ttttcgtgct taattgcaat tacttcaaaa cttggatcaa
    13141 ttgttagtaa gacttctttc attttccata agaatgcatt ctctatttca tggggtacat
    13201 ctaaataatt gtagccagca ttcattacat cacggcgcac ataatgcggt gcgtcaccac
    13261 ttatatacat gtcgtagcct tcagccattg ctgctggcca atcgcgactt ccgccaccac
    13321 caatgatagc gactcgctta atttcgcgct ccttatcacc agcgactagt aagccgtaag
    13381 ttgcttctag tttatcaaca atatagttag cagcttcttc aactgtcatt gggtacggta
    13441 aataaccgcc gcgcgccatt ggttcgctgg ctaatggttt aacggcgctt aattctaatt
    13501 tttccgcgag ggcatcattc atcccgtttg gtgcttcatc aaagttcgta tgcattgaat
    13561 aaacagtcac gggtaaagta tcaagccata gcgcaaatgc ttttttcttc tcatcgcgcg
    13621 caaacacttt agcgcgtgta cccctaatga acggatgatg ggttaaaatt aagtcaggtt
    13681 tatgacttaa tgcttcttca aggacaattt catctaaatc aagtagtaac acaatgcgtt
    13741 taacttcttc gactttttgc cctgccatat agccaacata atcattatgt ttaagggcga
    13801 tacgctttgg ataaagacga ccaagtttag ttagtaatgt gcgtgtcttc atactttttc
    13861 tccttcacta ataacttttt caataagttc ttgttctttt agcaactctt ctttacgact
    13921 tagcggtaac tttttattac ttaagatgcg tttattttgg ttataaacac tagtccacat
    13981 cttaataaag ttttcacttc tcgcttgtaa gtgataagtg ccaaaatacg gattaacttc
    14041 ttttgtcgct atcgcttttt gctcggcaac aattatttcg taaaacactt ctttctcgac
    14101 aacgatttct tcgtagctaa ttgaatagtt taacgcattt aatacttcgc gcactgcttt
    14161 agcgttacag tgcggcgcta aaatgagttt ttggacatta actaacttag cgggatgcgc
    14221 ctgtaagata cttacaatta attcgccacc cataccagcg ataacgacag tgttaatgcg
    14281 tgcaggtaag ttttcaaggc cgtcggctaa atcgatttca ataatgtcac tcttataagc
    14341 tttaagttcg ctttttaacc ggttgtatgg tcctttttta ttttcgctcg catataaagc
    14401 acgatgtcct ttaatcgcta aataattaac gagtaaaccg tggtcactcc caatatcagc
    14461 gacaacatca tcttttttaa ttaaattggc aattgcttgt aaacgcagcg ataacattat
    14521 tggatatgtc cgcgtaaccc tttcgtacgc gaaggatgac gtaatttttt aatcgcttta
    14581 gcttcaattt gacgaatccg ttctcgtgta acaccaaaaa tcttcccaac ttcttcaagt
    14641 gtccgcggtt tatcatcatc aaagccgtag cgtaagtgta acacacgttc ttcgcgtggt
    14701 gtgagaaatt cttgcatgtt atcacgtaat tgatcgtgta aaatgctctt tctaacaaag
    14761 tcggttggtg tttcattatc tttatcttcg acaaaatcac ctaaatgact atcatcttct
    14821 tcaccgattg gtgattctaa acttactggt tccatcgcaa ttgcttgaat ttcacgaatt
    14881 ctcgcaggtg taaattcacg gaatgattca cttacttctt ctggtgttgc tggccgttta
    14941 agttcttcag ttaaagtttc aactttcgcc ataatagtac gcactttatt ggcgacttct
    15001 tccggaatcg gattttcttc ggttgcgtta gcgcttaatt ccattgcttc ttcaacatcc
    15061 ttaacgctat atttaattaa agcatcacta atttcttcag gtgtagcgtc gcgcccaaat
    15121 tcttgggtaa ggcggcgctg tgtccggcta attttattaa ttgtttcaac catatgaaca
    15181 ggaatacgaa tagtgcgggc ttggtccgcg attgaacgac taatcgactg cttaatccac
    15241 caggttgcat aagttgagaa cttaaaaccc tttgtgtaat caaacttttc gacagctttc
    15301 attagcccaa tattaccttc ttgaattaaa tcaagaatat gcatgttgcc gctgctgtat
    15361 tttttggctt cattaaaaac aagtcgtaag ttgcgagtaa ttaattcatc acgcgcatcg
    15421 atatcgcctt cttctttata acgacgggca agtgttactt cttcgtcagc tttaagtaaa
    15481 ctatagcgcc ccatgtcttt catatagatt ttaacggagt ctgttgtttt aacttcaccg
    15541 agtgaaattt tacgtaaccg ttgattttct tcaaagtttt cttctttaac atctttaaag
    15601 gcatcaccaa atgataattc ttcttcatca aaatcatcat caagatcaag atcatcatct
    15661 aaatcatcgt caccatcaac aaaattagcg aaatctggat catattcatc gctttcaata
    15721 tcactatgaa tagtcacttt gttattttta aagtattgaa taagtttatc aaactcattt
    15781 tcgcttaagt ctaggtgtga agtcgcatcc ataacatcgt cttggtcgag ctcattatta
    15841 ttacggcgcg cttcttcaag tagaaacttc cgaatctgcg gtaatgttaa ctcaatttcc
    15901 acttcatcat cttcttcaat cgctacttcc tctaattcag cgaggtcttt taatttcttg
    15961 tcatccataa actatcgtct cctttactat ttcttctttc catatttttg cattaattca
    16021 gttttagctt gaatgtgctt aactttaagg gcggcataac tcgcttcatc aggcgcacgt
    16081 aagagttcgt cctgatgttt gcgttctaag atttgtaatt cgcgttcata attaagcgtc
    16141 ttagcggcgt catttaatat attttcatcg tatttcacat cttgcaggcc actttcaatc
    16201 gtgagtagct cttgcgtata ttcattaata gtagcttcat cagtgaaacg tgactgaata
    16261 tcatttaata aaagcgcata attaacttgc tcttttggtt taaattcttt taagtaatta
    16321 acgatatagc ggtgtacttc atgcgtaaac acgcgtacat ggttatcaaa gaaagtgcgt
    16381 gcttcgggat acacaagcat ttgataaaca ataaagcgtt ctgcctgcgc gactttattt
    16441 aatgtttgcc gcaccggtaa cttttgttta acagtattta tcgcttcgtt tcttgtttgt
    16501 tttttacgcc gtaagctttc atacatatca gtgagcacta aactattaaa gccgcttgct
    16561 tcggcaagtc tttcaatcat cgcacttact tcaagacggc tatttagttt taagatgatt
    16621 tcattcataa catcattaac aaacttcttt gtactagcca tatcatcaag ctttgtaact
    16681 gataagtaat aattaaaaat aaagtcatca cgcggaataa aggttgttaa ggctgttaca
    16741 agcgcttctt tcccttcttt atttaatatg tcatcaggat catagccgtc tttatgtggt
    16801 tttacgattt gcagcggtat attcgctgcg attaaatctt taatcacact tagcgtcgcc
    16861 atttgaccag cagtgtcact atctaaataa attcttactt ccttattaag taagcgtaaa
    16921 agcgctaatt gttctttcgt aaatgctgtc cccattaagg ctacaacact gcgttcacca
    16981 acgcggtcaa gagcaatcac atcaagaaac ccttcgacga gatacacata ttgcgctgcc
    17041 atcagtgctt ttgcgtgatg gtagttataa agtgtatccg acttaataaa taattgtgtc
    17101 tcacgcgtat taacatattt tggtgctttc ttttcgctag gcaacgctct tgcgctataa
    17161 gcgacaactt gtccgtcttt attattaagc gggaaaataa ttcgcccgtg attaatatca
    17221 taaggtcgct caagactacc acctaaaata ccaatatctt cgatcgtttt aagggcgtga
    17281 cctttacttt gtaaaaactt aatagtcgct tcaccgtcgc gcggggcaaa cccaatctta
    17341 tattttttaa gtaaggcatc atcaagcccg cgattaacga gatacttttt agcttcacta
    17401 cctgcttccg taattaaggt atactcgtaa taagcagtta aatcattaat cgcactgata
    17461 agcgggtcaa gctttgtatc gcgcacaaca aacttttgtt tttttgtaaa aaagtcatgc
    17521 ttaaaactaa ccatatccgc gagccgttga gcagcttcat agtatgtctt tttttcgtaa
    17581 agcgctacaa agcgaatcgc attaccgccg gtattacata cgaagcaatt aaagatttgc
    17641 tttgatggcg aaatcattag actaggatta cggtcatcgt gaaatggaca tagtgcacta
    17701 tagttttggc cttgtttatg gagcggaata taacggccaa tgacatcaac tatgtcaaca
    17761 ttttcgatta atagatcata agggctgcga gtactaataa gctaaacctc ctaaattact
    17821 actaattaat tactgaaatt ttacttttga gtgtaaataa gcttttaact cacttaatgg
    17881 taagcgaatc tgtgccattg agtcgcgttc acgaattgtg acgctttcat cttctaaagt
    17941 gtcaaagtca atcgtgacgc agtagggtgt accgattgca tcttgacggc gatagcgttt
    18001 accaattgag gcggtttcgt catagacagt actaaagtct tttactaagt cactgaagac
    18061 tgcaagtgct ttttcactta acttccgttg taagggtaat accgctactt gataaggggc
    18121 taagtgcggg gcaaggcgca taacaatccg ggtatctttt tctagttctt cttcgtcata
    18181 actatcaacg agagtcatga gtaataagcg atcaagccca acactaggtt cgacacaata
    18241 tgggatatag cgctcattcg tatctgggtc taaatattca aagttttcac cagaatgttc
    18301 catatgttgc tttaagtcgt aatctgtgcg gtcagcaatc ccccagattt caccccagcc
    18361 aaatgggaat aagtattcaa tatcggttgt tgctaaacta taaaagctta gttcatcttt
    18421 cgtgtggtca cggaacctta agtattcagg acgaatccct aagtcttgca caaatttaat
    18481 acaataatct ttccaatatt taaaccattc taagtcagta cctggtttac agaagaattc
    18541 taattccatc tgcgtaaatt cacgggttct aaaagtaaaa gtacctggtg taatttcatt
    18601 acggaacgct ttaccaatac ccgcgatacc aaacgggagt tttttgcgca tcgtacgttg
    18661 cacattttta aagttaacaa acatcccttg cgctgtttca ggccgaagat aaacttcact
    18721 ttgactatct tcagtaaccc cgatatgtgt tttaaacatc atattaaatt gacgaatatc
    18781 agtaaaatct tgtttaccac atactgtaca cttaacacca tactctttaa tgattttcat
    18841 catttcttcg tttggtaagc ccgtaacatt aagtccttta acttgatctt caattaagtt
    18901 atcagcgcga tgacgagctt tacagtttcg acaatcaatc agcgggtcgt taaaggttgt
    18961 gacgtgacct gtcgcgtgcc atactttcgg attcattaat attgctgcgt caatcccgac
    19021 gttatacggt gattcagtaa caaacttctt ccaccataaa ttgctaatgt tatttttaac
    19081 cgatgttcca agtgggccgt agtcccatgt attacttagt ccaccataga tttcactacc
    19141 ttggaacaca aagccacttg ttaccaagtg atttactatt ttttcaaatg aatgtttatg
    19201 cataattcct ccacaattaa agaaaagtat attattaaaa tacatttttg tcaacccact
    19261 atgagtgtag ttagattatt ttattaaatc tttactcata attttataat tataagtaaa
    19321 ttctaagtat gtaagcatca aggttaagag tgaaagtgcg gcttcatcgc taaattcgtg
    19381 acgcaagagt tcttcttcat taatcataaa tccgtagcgt aacatttgta tttcttcagc
    19441 gtttaaggtt attgtcttct catcgtagca ctttttacaa atgagtccgc ctttattaaa
    19501 agacacaccg atgatacttt tagtgtcccc acaatttaca cagcggtcaa ttactaaagc
    19561 actccctaat atgcgtaata aattcgtagt aaaaagatac actaacgaaa ttagttttgt
    19621 tgtttcactt agcgacatta acactttttt tataagcgga aacgcctcac taatcgaatc
    19681 attgtctttt agtgttttac taatgacttc accgatgata tttaaagcga taatacgctt
    19741 ttcgtcagta aaaactttag cgatattcgt taaactttcg acatttgtta aagtatgtcc
    19801 tgctttactt tcgcgtaact ccacatcaac taaagcatat aaaaagagct tacccgcaaa
    19861 ctttgattta acattaagtg ctccagtagc gcgaaaagtg ataatgcctt cttccttact
    19921 taaagcatta acaatataat ctttttcccg gtacggtgtt aaacgaataa tgactacttc
    19981 catctttagc ctaataattt ctttaatatc tttgggtcac ttcgccataa cggttcaact
    20041 ttaaccatta attctagtaa cacatcttta cctaaaaact ttgataattc atattgcgtt
    20101 tgtaaacgga tgcgtttaat cattttcgct cctttaccaa taagaatgcc cttttgacta
    20161 tctttttcgc aataaattgt taaataagct tcaactttag tgtcactctc tgttaaatga
    20221 tcgacaacaa ctgcgacact ataaggaatt tcttcgcgtg ttaacataat aatcttttca
    20281 cgaacgattt cattaatata attttctaaa cgaattgcat aatcttcggg caacggaaaa
    20341 taaagtggcc ctacttctaa atgttcaaag attaatgtta taagtgcatc aatattaaaa
    20401 ttacgcacac tgcttacttc aataagtggt gtatccggat aattttgtgt ccattgctct
    20461 ttaacttttt gcacgtgaat gggagaagct aaatcaatct ttgttaagac gataattaaa
    20521 ggatgatcaa ctttaagccg actaaataaa tattcatcac ccgcaccata ctccacacta
    20581 gcgtctacga ctaaaagtgt tacttcactc ccacgaatac tctgataagc ttgtttattt
    20641 aaatattcac cgtatggtgt ctttggttta tgaatacccg gtgtatcaat aaaaacaatt
    20701 tgaccacgta agtcgttata cacaccttga atatttttat tagttgtttg cggttttggg
    20761 gtaacaatcg ctcttttttc acctaaaatc gtgtttaaaa gtgtcgattt accggcatta
    20821 ggtcgtccag caatggaaac aaagccactt ttaatcataa gtcactatca tcaaaggcga
    20881 atggtaaaag ttccgcaatt gttgtttcta aaaatgcatc ttctaaattt gttaagtaaa
    20941 tcggagcgtc ttttggtaaa agttcactca tcacttgccg gcacgctcca cacgggctaa
    21001 ttggtcccgc tgttttacca gtgaccgcta gtgcgacaaa gtcttctttc ttttcgccat
    21061 tcatcattgc gtgaaataaa gcgtttcgct ccgcacaatt acataatccg taactagcat
    21121 tttcaacatt cgcgccgaca tatactttcc cgctttttgt taatagcgct gcgcctactt
    21181 taaatttaga ataaggaaca taggctaagg cacgtgcttc tcgtgctttt ttaattaact
    21241 cttcttttgt cactttttcc ctcctctttt accaataatt tggtcttgca aagtgatcat
    21301 cacctgctct tctttttcat ttgtgtgatc ataccctaat aaatgtaaaa gtccgtgcgt
    21361 aaataaaaat ttaaattcac gccgggcatc gtgtcctaat tcaagcgctt gacgttgcgc
    21421 tactggggca caaataataa tggaacctaa atgtacaaag ggttctttaa acttatgacc
    21481 aggtttaata tcattaaagg caaatgataa tacatcagtt gctgcatctt tagcgcgata
    21541 ttctttattt aatgccctaa tcctttcttc atcagtaatt gtaacttcaa caaggaattc
    21601 acctgtcata taatcaagta atttcattgt ttttgcataa attcgtttaa aaatagcccg
    21661 atagttttta taagtcggtg cataatcatc ataaaattca acagtgagtt taagcatcaa
    21721 aataatttta ccatagattg attaaattaa cgcagtgctt taatcgagta ataaagtaaa
    21781 ccactaaagt ggtatttgaa atatacatag tacaatgtta agataatgta tataaaattc
    21841 ttatttgtta cattcttaaa aacaaagcat taaaagaaag gaaatagcaa tgaaatctga
    21901 ctttaccttt atatattccg caccaaaagt tacagatgaa aaattaccag cggttttttt
    21961 atttcacggc tatcgaagcg atgaaaaaga tttactttca cttgttgcaa gtttagaaag
    22021 cacccatcat atttttagtg tgcgcggacc gctcagtttc atgtttggtg gatacgcatt
    22081 ttgggacatg caagacttag aaaaccctaa tcttgaacaa tatcaaagcg cagtggaaag
    22141 tgtacaaaat tttatagtta atgctatcac tcaatataat ttagaaaaga aagacatcac
    22201 gcttttaggt tttagtcaag gcgcagtact tgtgcaatca attgcgctca gggcacaaaa
    22261 actagtgaat aaagtcgtag cactaagtgg tcatcttcct tattttgcta aggatgaaga
    22321 atatgcgaca ctaaatctta acaaactaca tatttttatc tctaacggca ttaatgataa
    22381 tgttgtgcct tacgcttggg gtaaggaaag cgcggactat tttaagaagc gcggcgctga
    22441 tgtgactttt aagtcttatt ttagtggcca ctatgtttcg cgtgacaacg cccgcgattt
    22501 aatcgatttt atagtaagta attaatagtt aatcatacat cttttaatta accggaatat
    22561 cattaatagc gttgatagca ctataataat gatgagtatt cagcgctaaa ctaaagagct
    22621 ttttaagttc atataagtaa taactgaagc caaagaaata aaatattaag aaattaagtg
    22681 aataaacagc ggtaaaatac attaagacaa agattaagac cccaagacta aaatgaataa
    22741 taatcgaacg atatacccgc cacgcggcaa tatgcgttga atcattaatg atttccttat
    22801 ataactgcat tcttttttca ttattttctt ttgctaaact acttaattta acttctaatt
    22861 caaatgtttt agcgctacct ttaatccccc ttttaacttc aattaatgct acaatcatca
    22921 aaagcacact gatgattaca aagtagtaat tattaagcac taaaataatc gttataagta
    22981 atgtgctaga gatagcacta aaaagttgta aaggtctact aaaaacaaca gttttagcct
    23041 cttgcaatac tttaaactta tacttaaagt tatctttaat atttacgagg cgtggcgcta
    23101 tatataagtc atcgaacttt tgtaagttct tcattattac ttgttgttca ataatcttta
    23161 atatcgctgc taaagtaaat gacacataac ttattaaatc taaacttgca tgacctagga
    23221 aagcaaaacc aattaataaa aagattaaaa acaaagagtg aaagattaat gcaccatatc
    23281 gttgtttgaa tttgactaaa ggtggatttt caaccttctc tacacaatta actactaata
    23341 tattactaat aaaatacttt ttaatgtttt ttagacttaa aacataaaat tcaccacttg
    23401 gatcagcaat gctgagtttg ccctttttaa actctgctag gacgaaatga ttcgtatcat
    23461 ctttagttaa atgcataata aagggaacct ttacgttttt aacttcttcg atactatcaa
    23521 gcttatatcc aagtaactcg acaccgtact cttttgcctt tttactcaaa gctagaaagt
    23581 tatcaaagtc cccacttaaa ggtaatttta aatagcgctt atcacgatga gtaatcgcaa
    23641 gcagcatacg gagcactgct ttaccgcatt cattagtgtt attttgggtt aatcttacat
    23701 caattaggtt cacacttata attacaacct aatcgtttat tttggtacaa aaaaagagaa
    23761 gaatgtgact tcctctcttt ttgcgctaaa ttaattagcc tctgcgggct ttacgacgag
    23821 cttgttccgc tttaagtttg cggcgaagcc ctggtttaac atagtattga cgttttctag
    23881 tttcagaaag gacacccgct ttatttactt gtcgtttaaa gcgtcgtaat gcttcgtcta
    23941 atgtttcgtt ttcacgaacg acggtttttg gcatgctctt cacatccttt cttggctagt
    24001 tgccattaat tattatagtt aattattaat ttaatgcaag aaaaagcata ataagaaaaa
    24061 cttagtatgc taaacgaatt taatgtgtta ttaacctgtt atttaaagtt cttattatca
    24121 agttcaatat tattcttttt caatatgtaa ttgtttaaat atatcctgct gatattgtac
    24181 caaatacttc taaatattat ctaatagtaa tttttcttat ttttgcaatg ttctaatatt
    24241 ttattaattt gtttacgtga taaatcgtta caatggattc gttttactga accgtctaaa
    24301 taattaaatt caataaattt atgacctgtt ctataagttt cgatacctct acctcttttt
    24361 tctcggctgc gcgaagaaaa tttgtaatct cgttgttgta cttcaacaaa agaaacatct
    24421 ttaagatcaa tgttatactt atactccacc ttaagaaggc cgacaagtaa atcgccactg
    24481 acaaaaatag aagactcttt taaaataatt attttgattg caaataatat taaaaatatt
    24541 gatatcggaa ttagaattat aaaaaagaat tttgtccgcc acaaaaaaat atctaagagc
    24601 gcaaaaagca aaaaacaatt aacgaaataa tcattttaat tagcgaatta aaaggaaata
    24661 tataatcttt catttatcac acttcaatac tacatttaat tagcaattaa agatatcaat
    24721 gaaactatta tcgaaattac caaagaactt actaaaaaaa ataaaattcg gcgcgaatta
    24781 aaatacgtta tttttgcatt taaacatttt ttcttaatat tgataaccat caatagcggt
    24841 gggttttcta tcgaaattaa gtacttgtgt tctttatcaa aaagcactaa ctctgtcggg
    24901 cttggttgac catctatcgc accgaaatta tatgtcagat aatatacatc tttaagatat
    24961 attgtgctgt gcacctcatt atttatttgg gtaattaaag aatcgctagt aattatataa
    25021 gctgttttta taaatatttt tgcaattgcc gcaataatgt agaaaatgat attgactata
    25081 acaatcacta atatcgttaa accagtgaac ttaataaaat ctttaaaaat aagcgggggg
    25141 aataataata atgaagaaat taaataaatt gggataaaag taattattag ttcactaatt
    25201 acacctgcaa ccttaatcat tatattccgc ttatattttt tcatgaaacc tattccttta
    25261 ataatatctt cataatcttt tttatttgac actgagcata gttaccaaca tatacatatt
    25321 tagtgccacc atttttgatt ataatttcta aatatttatt gcgtatgtgc ttataaaata
    25381 ccttgtgctt tttttcttct ttcgtcaatt taacaatgtt tactgcgagt acttctgata
    25441 taaaaagttc ttggttccac ctaatatcaa tattatttat catttttttt ccacgttttt
    25501 gcatgcggaa aataatagaa aaacacataa tgattattca ttattaatgt atagccagta
    25561 atccaaaaaa taccaaccac aggaagtgaa acccccaagg acgcaaagat tattaaatac
    25621 cagttcttgc ataaccactc aagattgctt gtatcagcaa aaacaaaaaa gaatattgaa
    25681 catatcgtaa agggtgtgct aatgatagca acaatcagca catcataaaa tgcaattatt
    25741 cgttttttca taaatctcct atgtggcaat tttatcaatt aattgaccag ttattaaata
    25801 tttggcatta tttcttactc cgtgattgag cgaacttgtt tgaaatactc ttccaccttc
    25861 attagaagcg tatgctcctc cacttgtacc ttgtccgtag cgctttacta agttgccata
    25921 atcatcatat tcattatttg ctgaaataat accatgcgtt tttttaatta tattatcgtt
    25981 ttcatcatat tcataacttg ttacaattgg ataacttttt acgataatgt ccgctaggta
    26041 gatggtcgtg tttagattgc ttccaccatt aagtactatt cgtacttcat caaatatatg
    26101 tctggccgta atttcagtag ttaataagtt ataataactt cgtggtcatc actaaggcat
    26161 caacactttt aattaatgtc tttatttctt tatttgtttt ttccttaatt agtgactgat
    26221 ataaaaaaag agtggaatcc cctatttttg tggtttccac ataatcatca ataaagttta
    26281 ttgttctgac aataactttt tcttcactat tttttggttc tacttaattt ctaattaacg
    26341 agtaatatcg attaatatca taatcaccac tattaacgga ctttaagaga tttaagagca
    26401 gtttataatg tctttttttg atgttctttt cactatattt atatttaagt tttaagtcat
    26461 taattgtata tattttcatc tctttctcct aacttaaata tagcataaga actaaatttt
    26521 tcaaagataa tttaggttaa tataaaccag taaaattatt gataccacaa ttagataagt
    26581 taaaatttat ctaacaatta aacttaatcc attgctttaa tacgtttaat gcgtttaatc
    26641 gtatttaaaa tataacgcca taatggcaca agtaatagga tcatccacgc tggatgccat
    26701 ttacccgtta ccgcaccaac tacgacaaag gcgaggataa ttaaagcatc aacggaaaat
    26761 aaggcgattt tcttttctaa aatggctgtc actaatgaat caacgacagg aattgataag
    26821 aaaataaccc aatgtggatg ccatttatca aggaaaaaaa ccattgcgag gtaagcaata
    26881 acgacaagcg tcacatatgt cgacattact aattttctaa tttcaaattt cttgcgtcga
    26941 ctcattccct ctttattttc ttcatcaatc acttcaactt taacaaattc agctgtatca
    27001 ctcataaatg tgttcctctc tttttataaa tctaggcgat atatcgcgag gggctctata
    27061 cctttataat aatacatata attttcaggt ggtgtgacaa tttctaataa ctttgcaccc
    27121 attttctcaa aagtgcgtaa ataacccttg ctcgaacttg gcgcactaaa gataacgtat
    27181 tccatcttgt ggtactttgc gacaacaaga attgcgttta atgctttaac cgcaagattt
    27241 tgtccgcgat acgctccgct aacttgataa acaaagttcc cactgtagta agaattaaaa
    27301 ttatggccga tgcgcactaa gatgttcccg actaaggtac cgtcatttaa atgaatacca
    27361 tatttataat aaggtaaacc accaattaag ccactatttt tctcagtaat acttaaatag
    27421 atgtcttcac caataatctt gtcaaagtct tgattaaact gcatatttat cattcctcct
    27481 gtttcattat aaacggaatg attatttttc ttcgtgcttt tgcttatccg cactttcagt
    27541 catatcaaaa taacgttctg gatgattagt gataaaagta ttaaacattg ttttaagcgt
    27601 ttcttcagcc tttttatgat cttttaagaa gcgcacaaca taaagttcac tatctttcgt
    27661 tgcaatcact aaattacggc gcatattccc aacagagcta aaattactaa acgctacttc
    27721 ttttgtcttt ttcttactta aatgtaagta agcgccttct tcatcaacac tgagtactac
    27781 tttaggacga atgaggtaaa taattaaaaa caatgcttcg ccaccaaata aaataaggaa
    27841 aaggaatgat agtaaaacaa tatcaccact tgtgctacca gggtttttaa cgatcatcac
    27901 gacccacgaa ataaacaaag taatcgttac aagtaaaacc ccgacataat aaaataagta
    27961 tcgtttagat ttttcaaaag taatcataaa tcaagtgttt tttagtaacc ggtacctttt
    28021 ttaccagtaa agaaagcaac accattactc gtgccaattc gattagcgcc agctttgacc
    28081 atcgcttcaa gatcttccat agtacggacg ccgccactag ctttaacgcc catatcactc
    28141 ccaacagtgt cacgcattaa tttaacatcg gctactgtgg ctccgccggt actaaaacca
    28201 gtactagttt tcacaaaatc agcaccagct ttacggctta aaagacacgc tctaactttt
    28261 tcgtcatcag ttaataaaca tgtttctata ataactttaa gaatgaccca atcaggaagt
    28321 gcttcacgaa tcgcccggat ttcagtaaag acttctaaat ctaagccagc tttaagttta
    28381 ccgacattaa ttaccatatc aacttcgtca gcaccttctt cgaccgcaat ttgcgcttca
    28441 agaactttcg cttcagtgct tgttgcacca agtgggaagc caacaacagt acacacacgg
    28501 acatcacttt ccgctaaaag catactcgca agtggaacgt accccggatt tacgcataca
    28561 ctcataaaat cgtattcaag cgcttcttga caaagttttt cgatttgtgc gggtgaagca
    28621 tccgctttca gtaaagtatg atcaattagt ttattctttt tcatattacc ctccaaatga
    28681 tattttacca ttttagctaa tatttaatta cttttaatat ataaaacacc taaaacaact
    28741 atttttctac tttataagaa gttttgtaaa aggattttca aaagtgctaa aagttcaaga
    28801 gttttgtaaa tggattttca aaactttgga ttatttaaaa taaaaaagga agtttccttc
    28861 ctcatttatt tagcggttaa tacctatttc tttgctttgc ctttttttgg ttcttcttta
    28921 gtaatcacgt ctttaccagc atagtggtga cattctggac aaacatgatg cgcccgaatg
    28981 agtgttccac agtttgggca agtggtaagt ccagtcacgt ttaacttaaa gtgagtccgg
    29041 cgtaagcgtt tgcgggtttt actaccacgt cgttgttgaa cagccataaa cttatcctcc
    29101 ttcttgtgac tagggttatt atagcaaaag aaaaagtagt gtcaagaaat ttagacattt
    29161 tatagtattt attttattat attgattata tgctcataat ttagtgcatt atttttatac
    29221 agtgtctaga tttttttact aatattcggg tcataaataa tagtaattac ttcatgctta
    29281 atatcgctat cgcttttaac tctaactgct aaataattag tggtatgccc aacatatact
    29341 tcttctttat cgtcataagt ttcaattaaa acttgcattt tttcgccgac aaatctttgt
    29401 gcataacttc gatataagtc ttcacttaag tttattagtg tacgcacccg ttctttttta
    29461 actggaccaa gcacttgctc tttaaaacta gcggcaggcg ttctcgtccg cgcactatag
    29521 gggaagacat gtagctgcgt gaagtttact tttttaataa acgcgactgt ttcattaaat
    29581 aacttttcac tttctccagg aaagccaacg ataacatcag tggtaatagc aatattaggt
    29641 actttttctc taatattttc gattttatgt atgaattcac tcgtggtata atgccgattc
    29701 atcctgcgga gcacttcatc acttccactt tgcagcggga tatggagatg gcgtgctata
    29761 cgcttgtctt ttagcattaa acgaaggaaa tcgtcatcaa tttcatttgc ttcaatactt
    29821 gatactcgta atttatgtaa tgatggacaa gcttctaaaa tcatctccac aatattacta
    29881 aatttaatat tccctaagtc tttaccgtag gctcctgtat ggattcccgt taaaacaatt
    29941 tctttatacc ctcttcttac taagtcttta gcctccttaa caatgtcacg cacatcgcga
    30001 cttcgcatct tccctcttgc aagaggaata atacaatacg tacaaaaagc gtcgcacccg
    30061 tcccctattt ttaaatacgc gcgcgcctgt ggtgttacac tcgtagcgcc taactcttca
    30121 tagttaaatg tgcgtggctc ttcttcaacc gcaattattt tttgaccatg ctctttaaat
    30181 tcttcaatat aatcgacaat tttacttcgg ttactagtgc cgactaaaat atcaatttcc
    30241 ggaatggtat tgattaaatc tttgttaagc tgtgcgtagc atcccattac aactaaaata
    30301 gcatcaggat aatttttcgc gagtttacgg atatgttgtc tgcttttttg atcagcgtta
    30361 cttgtgacgc tgcatgtatt aatgatagca acatcaactt tgtcaccttg attagcaagt
    30421 ttgtaacctt gtgcttttaa agtttcttca atactaaaaa cttcgtaagc atttacttta
    30481 caaccaagtg cgacactagt aaaggtcttc atccaattat cttccgtctt tttaagttat
    30541 taatatattt agtgtataac ttatgactaa ttaacttttc tttcacaaga gcattaataa
    30601 aagcaatgcg attaccagcg taatattcag taagtgccgg tttaattcgt tctaatacat
    30661 tacggaaatc agggcgctta atatgtttat caaataaaca aatcttcaaa tcaagtcgtc
    30721 cttgatcatt aataatataa atcgcatgaa agtcataatg ataataagcg cgcgtatgaa
    30781 tatcttcacc aactaacaca agactagtaa gttgcgattc aaataaacta aaattatacg
    30841 cttcaaataa atctatcgcg tcatgggtta aatttagttt acttcctaaa ttattcccaa
    30901 ttaaaatgtt attgctaaca ctaaaaagcg ggcaatatat gggtacatta ttataggtaa
    30961 aaagtgcttt tactggtaat tctgtatcag gcttaacttc attattccag taattagtga
    31021 gtgcttcata atttaagagt gcactttgaa taatattcgc acagattaaa tcaaagtcat
    31081 ctttactatt aataaaagga ataagttcag gaacttgcgt taacagtgct tcatgatctt
    31141 ggaaaaataa atattcacga ctcggtgtaa taaggtgccc tcggcgattt tcctgtaata
    31201 actcataaat tttcatggtg cgctcctttt tgataatgat gtaacacact taaagcatat
    31261 atcgctgcgg tttcacttct aagtatatta tcgccaaaag taatgacatt aaagccttta
    31321 gtactcgcta aagtaacttc cgcttcacta aatccacctt ctggaccgac taaaatgagt
    31381 aaattatcgc ctaaatcttg ttgcaatact gctttaagcg gtaaattagc ggtttcatga
    31441 gcaataagct tagtgtcaaa ctctaaatct aaaacttctt taaatgctaa gggttcagta
    31501 agcgttggca gatagttacg attagcttgc attgctgcac ttttaataat cttattaaag
    31561 cgtgctaaac gactttctaa gcgcgttgca ggtattttac cgatactacg ctcactatca
    31621 attaaaatga tgtgactaac accaatttcc actgcttttt ggatcactaa ttcaagttta
    31681 tcgccttttg gtaaggcgta acctagtgtt aagcgcggta agggctctaa cggtaacttt
    31741 cttttttcta aaacattaag tataaacgga tttacacttt cataagtgca taagtaaaga
    31801 ctttgtcctg ccactacttc aaaagtttca tcctttttaa gccttaatac tttcgttaaa
    31861 tgaaaaatat catcatcact taagactact tcattatttc taactctgcc aaaaaacctg
    31921 taactcataa ctttaaatcc acatatattt tagcattaac gcataatgaa aaaagcactg
    31981 aacgctaaaa taccaacgac taaacaataa atcccaaaat aaattaagtg ttccaccttt
    32041 aactttttca tcacaaatag tcccgttaga aaagtcgcac caaaagtaaa gataaacgct
    32101 aagatgtaac ccgtccaatt aatttcttca ctaatatgta ctaagtcaac tgcgcctaac
    32161 acacttgtcg gtacactaat aataatataa gcaaggaaac taaatcttaa tatacttttt
    32221 agtgacacac gacttgcgcg accaccaatg atggtggagc cgctccgcga aataccaggg
    32281 aaaacggcaa acgcttgggt aaagcccacg actattgcgt ctttccacgt tatatcatca
    32341 tgttcctcaa ttaaattacg cgtaaagaaa atagtaagta aaagcgcacc agtaataaat
    32401 aaagcaatac caacagttaa tagattctta atcatcccca tctcatcttt aaaaataagt
    32461 ccgacaaccc cgattgggat cactgcaact aaaagtttta aaacatagaa aaaggtgtct
    32521 ttatactctt cacgcgcttc tttcttaaag atatacagaa aaaatccttt aattaattcc
    32581 catatatctt ttgcaaataa cacaaataag gctaaaaaac tcgcagtatt tgtaaacatt
    32641 tcaaaaacta accctggttg ttctagctta agtaattctt gaaagatgac tagatggcca
    32701 ctagagctaa ttgggaagat ttccgtgacc ccttgaataa tcccaagaat aatatattta
    32761 agaatttcca taactttcac tcactttgct tattactttg taattatagc ctattcttca
    32821 ttatttagat gttaaaaaag gttaggacgt gcttatccta acctttggct tcaagtttat
    32881 taactattag gcatttatcc aaattgctgg aagaataccc attgctgcat cagtgacatt
    32941 tatagcggca agggcgccgt tataatttac ccgcgaagca cttgtctcac tagaatatgg
    33001 ggaacgtgtc caccaataag ctttgtcata atctttacta acggtctgtg ataatgcata
    33061 atcacttgga taacgcactc gcgaagcatc atcaacaaaa ccataatgtc cgttcaaatc
    33121 taagtaactt agcagaaaca ctttatcaag agtatttgga caatcgaagt tatgcacatt
    33181 attaattgtt gtcgctttac tattatcaac agttgtcacc gcaattttat tttgttcagt
    33241 tgtagtaaaa gcagcattta aaaatgaaga atttaaccat gacctaatat gtgaatgttc
    33301 atagttatta gcgtacaccg tttccccatc aatatttcgt cttgtcttag cgtaaaaata
    33361 gtcttgagcg tcaagtacag tttgcgctaa aaggaaagct ttaccagcat caatatcaag
    33421 cactttccac gttattggtt catatttaaa ccagtaaaca atacgttttg tgtaaccatg
    33481 gtcttgttga ttaaaacatc cagcagtgct atccgtataa gtatagcggt actctgtgta
    33541 atagacaccg cgatattcct cgccctcatt tactaaatcg atataccaca taaaggaagt
    33601 caccgcacca ttatagtaat aaaggtaatc agtccacgct ttcgcgtttt cggcagttgg
    33661 taatccttca cttccgcctg ctaaattgtt tagtgtagta ataagcgtta catccgttac
    33721 tcttgtttgc gggtaagcac caaatttcat ttcttcccct tctgcgacaa aatcagcgga
    33781 taacacaaga ttattacttg gcatagcgaa ggtataagtt gcagagcttg acactaaatt
    33841 attactagcg tcaaaccacc ccgcaaagaa gtatggagca agtgcagttg ccgttactgt
    33901 cacattagtt ccagcgcgga ataagccctc actcgtgact gtaccacgcg catcattttt
    33961 actaagcaca ctaagtgtat gatagtgatc aacacagtca aattcaattt tgcctaagtt
    34021 aaaggaggaa tctgcaagcg cataaatccc tatatattta ggcttataac cattaaagtc
    34081 cgtgctaaag ttcgttggtg aactagcgtt aatcactact ttacgcgaag catcaaatga
    34141 cattgtgttg gaataaagaa tttcaacatt tacagtgtca tcgcgttttg ttaactctag
    34201 attcgtaatc ccgttaatcg ggtcactatt gacaatataa ccacccgttt taattctttg
    34261 ccagtgagtc gaagggtttg ttaaatgctt gtaagaaaca gcaatattgt tgcctagttc
    34321 ggttgtaagc actcctgttc ccgtataacc atcaggattt tctgatcctt ccgctatttt
    34381 gttcttctca gtaccaagaa caatggcgta cggtgttggt tcaccgcgcg cactagagcc
    34441 gtgattactc ccaagactaa tcgcgctaat aagtagtgcc gataacgcaa aaaaggctaa
    34501 ggtcgcctta ctaagcaact atttgttttt catagattat ttccttccga ccaaatgaac
    34561 atatgttcat aaatttagtt taacaccata aaatcaatta aaagttttaa tttcatgacc
    34621 taaattagta aaaagaagga ttaaaacttc acttaaaata aattaattgc atatattcgt
    34681 aataatttag ttgattatta actaaagcaa aaattcaatt ttttgtgata atgtttttta
    34741 tacaaaaaaa ggctagggat ttattcctaa cctctttcat ataattttaa taaattttag
    34801 cgtttaaatt tagatttaaa tttctcaaag aatgtttctt tctttggctc cgctttcttg
    34861 aagtcttcaa gcgccacttt ttgatcttta cttaaattag tcggtgtttt cacttctact
    34921 tctaagtatt ggtttccaac attaccgcgt acgtctttaa ccccttggcc acttaagcgt
    34981 aaagtagcgc caggttgcgt acccgctggt attttaactt cgacattgcc ataaaccgtt
    35041 gggacttcaa gtgtaacccc aagcgctgca tcgacaaagc taatggggat ttgtaaataa
    35101 atatcgttac caacgcgctt aaaactatta tgcggtttaa tgacaatttc aacatgtagg
    35161 tcaccattag gaccaccgtt aagtccgcgt tcacccttgc cttgaatccg tagtttttga
    35221 ccttcaccaa tgccggctgg gatgttaagt aattgctttt cttttacacg ggtatagcct
    35281 gcaccattac atgatttaca cggtttacta attgttttcc cgctgccacc acaagatgta
    35341 cacgctcttt gactttgcac acggccgaaa atactttctt ggacaaaggt ttcaaccccg
    35401 ctgccattac atttactaca tgtatggata tcgtttgcgt tttcagcgcc tttaccttga
    35461 cacgtttcac acatcttgtc ataagtgtgc ataaattcaa tctttgtgcc attaatcgca
    35521 tccataaagt taatgcgtac ccgcgttaat aagtcgttcc ctctagcggg tccagtttgc
    35581 cgactagcgc gtcgtccgcc gccaaagaag ccttcaaaaa tatcgccaaa gtcaaacccg
    35641 aagccactgc cactaaagcc accaccaaaa ggattacctc cgttttgatc aaaggcagcg
    35701 tgaccaaatt ggtcatatgt agcgcgtttt tgatcgtcat agaggacgtc gtaagcttct
    35761 tgtacttctt taaacttctc ttctgcatcc ttttctttat tcacatcagg atgatacttc
    35821 ttcgcaagtt tacgataagc acttttgatt tcatctttcg aagcactttt ctttattcct
    35881 aatacttcat aataatcacg tttagccatc ggcagctcct tttacataca gaaaccccgg
    35941 atggcggggt tcctgaaagt tgtttcttaa tttaaattag tcttttttat cagtgaaatc
    36001 agcgtcaatg atgtcatcat cttttggtgt ttcagtagcg ctagtttcac cttcggcttg
    36061 gccttgttgc atattttgca tcatttgtgc catttgttcg agttcactta aacgtttttc
    36121 aagcgtttcg taatcttctt tttcaatcgc ttcttttagt tcagtgacga gtttttcact
    36181 tgcttctttt tgattagcgt caactttatc gccaccttct tctaaactat gttcgatttc
    36241 attaatatat tgttcagcgc ggtttttaag ttcaacattt ttcttgcgct tttcatcagc
    36301 ttctttatgc tcttcagcgt cttttaccat gcggtcaatt tcttctttag agagtccgct
    36361 gctattagta atcgtaatat gttgttcttt ttgcgtgtct aagtctttgg cggttacttt
    36421 aacaataccg ttaacatcaa tgtcaaatgt tacttcaatc cgtggttgac cgcgacgcgc
    36481 tggtttaatg ccgtcaaggc ggaagtgacc taataattta ttatcacgcg ccattggtct
    36541 ttcaccttgg tagacaacaa tgtccaccga tggttgattg tcggctgccg tactaaagat
    36601 ttgtgattta gtcgtcggga tagtcgtatt ccgtgtaata agcggagtca ttacttcacc
    36661 cattgtttca atacctaagg ttaacggagt aacgtcaagt aagacaacgt ctttaacatc
    36721 tccagcaata actgcacctt gaatggccgc tccaattgag actgcttcat caggattaac
    36781 agtaaggtta ggtgctttac ctgtcatttt ctttactaac tcttggacag caggcattct
    36841 tgtactaccg ccaattaaga ttacttggtt aaggtcactt gctttaactt tagcgtcact
    36901 tagtgcccgg cgaattggtt cttcgactct cgctaataag tgttttgtta aatcaaggaa
    36961 cttcgctctt gttaatttac cttcgtaact aattggtccg cttgcgccca taccaataaa
    37021 gggtaaacta attgttgttt caagcatttg acttaagtca atctttgctt tttcagccgc
    37081 ttcacggaag cgttgcatag ccattttgtc atttaacttt aagccatgtt ctctttcaat
    37141 ttcttgctta atccattcga caatctccgc gtcccagtcg tccccaccta agtggttatc
    37201 tccagcggtc gcgataactt caaaggtacc atcaccaatc tctaagattg aaacgtcaaa
    37261 tgttccacca cctaagtcaa acactaagat cttttcatcg tgttctttat ctaaaccgta
    37321 cgcaagtgct gctgcggtcg gttcgttaat aatccgcaca acttcaaggc cggcaatttc
    37381 accagcgact tttgttgctt gccgttgcgc atcattaaag taagcaggaa cggtaataac
    37441 agctttttga atactttgcc ctaaattctt ttcggcataa tctttcatat aaccaagaat
    37501 tctcgcgctt atttcttctg gtgtaaattc tttctttaac gcattaattt taaacttttt
    37561 atttgtcccg atgtgacgct taacactcat cacagtatca ggattagtaa ccgcttgacg
    37621 tttggccgct tcaccattaa cttgttcacc atcaggttta aaggcgacga cacttggggt
    37681 ggtcggtttg ccttctgggg ttggaataac tttaatttgg ccgtcagctt ggcgatagct
    37741 tacgacacta ttagttgttc ctaagtcaat tccaataata atttctttct ttgccataat
    37801 atatatctct tctccttttt attaatttaa gtcctcagga ttaacttctg ggacattatc
    37861 atcatttttt ggttcttctt taatattcgt tgacactata acttgcgcgg ggcgaattaa
    37921 gcgatcaatg aatttaaatg tgttattgcg cacaccaagg acggtgtttg gttcaacttc
    37981 ttcactgtat ttaacttcca cagcgtgcat cgagttttca tcgaatttat cgccgacatt
    38041 cacaactact tcctctaggc cttcactagt taaggcatca agtagttgtc cgtagacgaa
    38101 gcggaagccc gttaagtagt tattaagcac tggatcttct tgttcttcgc gaatgacatg
    38161 attgaagata tcaagcgctg gtaagagttt ttcaataaat ggtacggcac ggtatttaat
    38221 gtaatattgt tgatctttcg cattttgttt acgcgcatta tccatatcag cgagtacgcg
    38281 catatatttg ttatgccact cagcactttc agcttttagt ttttcaattt cagcttcgag
    38341 tttttgtatt ttcgcgagat ggcgtttttt tggcgctaca tctttttcca catgtttctt
    38401 ttcatcactc atcattaatc tctctttcta tctcgaagta cttttcgagc tcatcagtta
    38461 cgtattctaa cgcccataag actttactgt agtccatccg ttttggacca actaggacaa
    38521 tttgtccgac ctggtcgcct gggacatgga cattggcgct tatgaaggtt aagtcttcat
    38581 ctttgccccc gatattcaca cttaagtcac cgtttcccgt gataagttca cgtattttct
    38641 ctggatcatc aaggattgat atgagtttac ggagcttttc actatcatta atgaattctg
    38701 gttgctcaag taagctttct ttaccgtata agctaagccg gtcacgggcg taccttataa
    38761 atgcatcagt gaaggcattt gctaagactt cttgttcaac aatgtaatcg gttaagagtg
    38821 gttgtaagag ttccatcttt gccgatactt cactaattct tgtaccgact aggcgttcat
    38881 tgaagatatt agcgactttg ctaatttctt gaatacttgt tgaaggcggg acgatgaagg
    38941 tattatgttc gacatagccg cgattagtga caaatattgc cgtccccatg tttttatcta
    39001 acgcgacaac ttgaatactt tgtagtgttt cacctttttt ctcgggacca agtgcgacac
    39061 tgacaagatt tgtcatttgt gagagaattt cacatgtttg attaattact tgttcaacgc
    39121 ttttcgtctt ctcttccaca agcgtcgcaa tgtgatgtcg gaagttatca tttacttcat
    39181 cttcacgtaa gttagcaaca tagaattcat agcctttctt acttggtaca cggccagcac
    39241 tagcgtgtgg cttttcaaga aagcccattc cttcaagggc attcatttcc gctcgaaccg
    39301 ttgcactgct atagtttaag ttataattat cgattaatga ctttgaacca acaggttggg
    39361 cagtcttaat aaataactca acagtatact ttaagattaa ctctcttcgt ctgtatctca
    39421 taactcctcc ttagcacttg caactttcga ttgctaaata tattatacta aagaaaatag
    39481 cactgtcaac atttaagtgc taaatttcgt aaatttttac taattacttc gtaaaaagcc
    39541 aaaaattagg ctattttttt gttaaatttg cttctttttg gttttttaat cgcatgtttt
    39601 tcattaaaat ttaagagccg taaaccgttt aatatcacta aaatagtact accttcatgg
    39661 atcgctactg ttaatcccat tggcaatgtc accacgaaat taagcgcaat taataatagc
    39721 accacactga gcgctagcac aatattttga ataacaatgc gtttaagttt tcgtgaaata
    39781 gcgtgcatta atgataactt ttggaaatca ttgttgacaa gcaccccatc agcgacatca
    39841 attgccacat cagtgccatc ccccattgca atcccgattg tggcttcaac aagcgcgggc
    39901 gcatcattaa tcccgtctcc caccatcgct actccgccgt attccttttt tagttcttta
    39961 acaatatcaa gcttttgttc aggtaataca ttcgcgtata ttcgcgtaat gcctaactct
    40021 ttaccaatag cttttgccgt taattcatta tctccagtaa tcattactgt attaatttct
    40081 tgttttctaa agtattcaag cccatcaaag gcacaacatt taatcatatc taaaagcgct
    40141 ataagtaaga ttggtttccc gttttttgca ataaagacga ctgttttacc ttgaactgcc
    40201 aactctttag cgcgattagc gcagcgctct ggtaaattaa ataatgctgg tttacctaca
    40261 agatactcgt taccgtcata cattgcgcgt aatccactgc caattttatg ttcaacttct
    40321 aaatcaactg tttttatagg agtaaaatga ctaacaatcg ctgtcgctaa tgggtgatta
    40381 cttttctttt ccatactgac aaggaaaact tttccttctt ctgcttcttc tgcacttact
    40441 cctggcggga tattaacatc agtaacactc ggtttccctt ccgttaaagt accagtttta
    40501 tcaaaagcaa ctgcttctac atccgcaaat agagctaagg aagaagcacc tttaaaaagc
    40561 acaccgcgtc ttgctaaaag acttaaagca cttagacttg ccgggatatc actaacagct
    40621 agggcacacg gcgaagcacc gataaaaaga ataattgtgc gtttaagcgc aagcgtcatc
    40681 ccgttagcgc taataacttc attccaccct aaaacataat agccaaataa ataaaatagt
    40741 ggagcaatta acatcacaat agttacataa attggttcaa atttcttaat aaatgtcgca
    40801 gttttagtta aattagtttg cgtttcactc acaaccctta agattttcgc taataccgtt
    40861 tcatcactat ttttggtcac taccatcgta aaagtactgt tgccattaat actaccacca
    40921 aagacttcat caccttcatg cttctctttt ggcatacttt cacccgtaat catcgcttca
    40981 ttaattgcgg cactaccact aactatcgtg ccatctgtcg ggatttgatc accatttaac
    41041 acaagtactt tgtcaccaac ttttagtgac tcaacactta cttcttgttg tgttccgtct
    41101 tgaagtaaaa gcctagcggt tgtcggtgtt atctttaaaa gattcgtaat ttctttggca
    41161 ctttttgctt cagcatattc ttcaagaaaa tacgcgcccg cgaatattaa tataagtaaa
    41221 gcagcgtcgc cataatcacc aataataatc gccccaatcg cccctagtgc cattaagata
    41281 tgaatatttg gacgaaatct tttactttta atactctctt taaccgtttc gactagtcct
    41341 tcactaataa ctttgtaacc cgcaagaata agcgctatta agtttaatat cattaccgct
    41401 ttatcactta aaaaactcac accggggata aatgctaaaa gtgcaaataa aaagagcaat
    41461 agcccgataa aataagcata gagaccaaac tcgtgaaata ttttttgaaa tttactttgt
    41521 ttattatctt gtatcatatt tttactccct aatatggtca cgtgcttgtt taataatttc
    41581 ataaatatgc gggtcaagaa tttcataaat ccgctcttgg ccagcttttg aatacgtgac
    41641 tacgcgatgg tcataaagtg tccgtaattg atgactaatc gcactttgcg tcatatttaa
    41701 tgcattagcg atttcagtga cattctttgc gctttcttct aagagtaaaa taatacgcat
    41761 acgcgtatca tctcctaaga ctttaaagaa gtctcgtaat ctttcaatat ccacgacttt
    41821 atccataatt ttctcctaac acttgaatat ttgagcaagt gttcatatac attataaaaa
    41881 atatatctaa tatatgtaaa gatatttaca ttattaatgt taaaacgact tgatcaagga
    41941 gcataatccc ttcatctgtc gcatatactt tgtcatcagt gacatttaaa agtcctcttt
    42001 ctatgaggtt ttttacctta gtgccatact tttctaaaaa atcaaagccg acacgagttt
    42061 taaagtcttg taaactaaaa ccttgtgcga gtcgtaagtt aagcattaag tactcttcaa
    42121 catattcttc gcttgttatt tcgtcgtaat ttatgatact ttcgcctttt aaatacttag
    42181 ttaaaccgcc acttattaag tagcgtttac catcaatata cccactagcg ccaaggccaa
    42241 tcccgtaata attacggttt tcccaatata gtttattgtg gatactttca tatcccttta
    42301 acgcaaaatt actaacttca tagcgtatat aattattatt tcttaatgtt tttaaaataa
    42361 agtcatacat ctcgcgtaaa gtgtcgtcac ttggaacttc ccacttttta agtgcagcca
    42421 tcgtgccttt ttcaatcgtt aagctatagg tagaaatatg tggtgctcct agctttatga
    42481 tgttcattaa gtcattttct aagtcgctaa gagtttggcc aggtaatccg taaattaaat
    42541 caacactaaa gtttgtaaat aacgcctttt taacaagcgc aatctttgtt ttaatcatcg
    42601 tataagtatg atgacgattt aattttttaa gcatatagtc atcagtggtt tgcactccaa
    42661 tactcagacg attaacaccg taacttcgca ttaatttaag tttaggcaag gttaaacttt
    42721 cgacattagc ctcgactgta tactctttta cattagccgt ataaggcttt aataattcta
    42781 aaagattacg taactcataa tagcttaaag acgaaggcgt cccgcccccg atataaagcg
    42841 tacttaagtc acgactaaca ttaaaaaaac ttaagtcatg cttaagcttt tctaagtaag
    42901 atgcaaccca ctttttttga taaattactt taacaaaatc gcaataatgg catagtgaag
    42961 tgcaaaaagg aatgtgtaca taaagtccac gtttagtcat ttttcgtttc tttatctact
    43021 ttttcaaatt ccgctaagat tttttcatca gtaattggtt caagtactct atctaattct
    43081 tgttgaacag cgacttgttc attaacgcga ccacctttat cctttaaacc aggaattaag
    43141 cggcgaataa taaaggtaat aagcgcaatt aagccaaaaa atagtagtaa aaataacaga
    43201 attaatcctg gtgaatcaat cgcaagtgtt ggtaaataaa tcataatcat catcctatgc
    43261 tttccgtgtt ggtgtataaa taaatttgtt tttaaatttt tctttgcggt aatatccgct
    43321 gttagcgagg tgttccattg atgtccgcgc tgtttcatac gcacagcctg tcacttcttt
    43381 aaagtcatta attgagtaat atttacccat agtacagtgt ctagcgtaga aataagcttg
    43441 agcgcgactt aaacttggat cgctttcacg aatatgtcgc tctaagcgta atatatcatt
    43501 ttcgctaagt tctggtggta attgtgtaat ccctaaattg gcctcttctg gtgtaacttt
    43561 tacgccttct tcgcgcggcg gaagtggaag cgggattgcg gatttttttg gtgtataaac
    43621 tggtactggt tcagatttaa aagtttcttc cttctccgct acttcggctt gggcatgttc
    43681 aaaaagatca gttggtggtg tagcactcac actttcttct tgcgtttctt cttcaaatgg
    43741 tgggagcaca tgcttttctg cttcaagttc atgcgtcacg acaaacacaa tccgttcttc
    43801 aattgtttgc agtgattcat taagcacatt aataagtaaa tcaataaaat aagttaaatc
    43861 agcacttttc tttacttcgg cgaaaacatc atcacgcgca tcacggtatc tttctaagat
    43921 aagttcaaag ttaagataag gcgcaataag cgcgagttct ctttctgcaa ggaaggcttt
    43981 tgccattaaa atggcaataa tttcactatg agcttcaaaa ggtttaatat aatcaagata
    44041 gaaaataatc gctgcacttt gcacaatggt tggtaatgta cttgtggcca taaatgcgta
    44101 aaaatcagcg attgcatcac taatttgatt gacgggaaca tgcggatgaa catgattaat
    44161 gacggagact tgacgccccg ttttaatttc ggtagtgcga taaatatagt tacttagcgg
    44221 cggaacatta aataggcgcc caacatcagc taaaagtgct tcagtaaaat caagattagc
    44281 gctttttgct actagttcta acccgcgata ataatcattt aaaaacatct cccacgcatt
    44341 taaaacacta atattgtgat taatgagttg attaacaatc ccatcatgaa aattaatttc
    44401 ataactagcg gcgattgttt ttaaaatatc acgagttaat aaggttttaa gtgccttttg
    44461 atgattagcg tcaacataaa cttcttgaat tcgtgcttga atacgcgaga ttcgccggtc
    44521 aagttgattt aagcgatcat taattttggg ggttaataca acgcgtaaac gattacgttc
    44581 aaccgtgcgt aaagggatgg tcacactgaa catattgcga tattgtaata cttctttcca
    44641 aatatcatca atcagtgttg tgccaagtgc cctactaact tgattcatcg ttagatagcg
    44701 ttcattacta aagcgttctc cttggttagt gctcatacat ttcttcctct attaaagtgt
    44761 accataattg atgtttgttt tgacccctaa aactataaaa ctactaatat tttactaatt
    44821 atcatcatca atacttaaaa ctgacatgaa ggcttcttgt ggaatctcca ctgaaccgac
    44881 gagtttcatc cgttttttcc cttctttttg cttttctaaa agcttgcgtt tccgactaat
    44941 atcaccacca taacacttcg ctaagacatc tttacgcact gctttaatat cggttcttgc
    45001 aataatctta ttattgatag ctgcttgaat cggtacttca aattgttgtc gtggaatgat
    45061 gttttttaag gctcgacaca tggcaacacc gcggttataa gcagtcgggc gatacacaat
    45121 tagacttaaa gcatcaactg gttgaccatt aagtaaaata tccattttcg ctaagttact
    45181 agctttatag ccgctaagac tataatcaag tgtcgcatag ccctttgaat aactttttaa
    45241 tttatcaaag aaattgtaaa ttacttcaga taagggaatc tcataagtaa cttgcattcg
    45301 cgtatcatca agataagata aatcgacata aataccgcgc ttatcttggc ataattccat
    45361 aatgggaccg acgtattctt gtggtgtcat aatttgtgct ttaacatatg gttcttcgat
    45421 acggtcaaca agtgtaaaat ctggtaattt acttgggtta tctattttta acatcgtccc
    45481 attagtgaga taaacatgat aaataacact tggcgctgtt aagattaaat ttaagtcgtg
    45541 ttcccgttca agccgttctt gaattacatc catatgtaaa agccctaaaa atccgcagcg
    45601 gaaaccaaaa cctaaggcaa cactagtttc tggctcatat tttaaggctg cgtcatttag
    45661 tgaaagtttc tctaaagcgt ctttaagatc accataatcc tttgaatcaa ctggatagag
    45721 accgcaatat accatcggcg taatttcgcg gtaacctggt aaagcttcct ttgctttatt
    45781 attaactaaa gtaacagtgt cccccgtact aatatctttg atatctttaa tttgcgcagc
    45841 taagtaccct acttccccag cgcgtaatac atcacgcttt acttcttgcg gtgttctaat
    45901 ccctaattca gtgactaaat actttctatt cgtcgccatc attaacactt catcacctac
    45961 acgcacttcc ccttctttaa cgcgcatcat aataatgaca ccgcgataag ggtcataata
    46021 actatcaaaa ataagtgctt taagtggtgc tttaacattt ccgcttggtg caggcacata
    46081 tttaacaatt gcttcaagta cttcttcaat attaaaacct gttttagcgc tgacgccaat
    46141 aactttagag agatcaagac caatccgttt gtgaatttct tcacgcactt cttccactct
    46201 tgcacttggt aaatcaactt tattaatcac tgggataatc tctaaatcat tatcaattgc
    46261 taaatacata ttcgctagtg tctgtgcttc aacaccttgg gtcgcgtcaa caactaaaat
    46321 cgctccttcg cacgccgcta aagaacgact aacttcataa gtaaaatcaa catgccctgg
    46381 cgtatcaatt aaatttaaac gatatgtttt accgtctttc gccaaataat caagggcaac
    46441 agcattaagt ttaatcgtaa tcccgcgttc tctttctaat tctaaagaat caagcacttg
    46501 cgctttcatt tcacgctttt caagtgtgcc tgttgtctca attaagcgat ctgcaagggt
    46561 cgatttacca tgatcaatat gggcaataat tgagaagtta cggattaaat ctttgttgta
    46621 attcataata cttactcttg agatagtatt ataccaattt ttattaaaat tgactaatga
    46681 gaaaagacta cgttaaagga aaaggttaaa tttacatcct tcttgtcttc attataatgt
    46741 ttat
//
LOCUS       DAOSLP010000004        42984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_1041_length_42984_cov_12.858674, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000004 DAOSLP010000000
VERSION     DAOSLP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 42984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42984
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1041_length_42984_cov_12.858674"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1459
                     /locus_tag="PK460_00820"
     CDS             65..1459
                     /locus_tag="PK460_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOE53680.1"
                     /translation="MITKKLALEVLNVALSTGGDFAEIFLEETYSGNYTVENGKTQAP
                     ATSLTYGAGIRILKGLQSVYGHTSDLSKKGLLNLATALSAGFDGQRVLSVEKLKNVRV
                     RKNHELEKSLELVDINEIVEKLKITNKVMKDYDEKIVRAISVLLTDVRNITIFNSEGN
                     AFTDKQERVRLVFQAIAMAPGAFEYSYNGPGAHKGFEYVEGLDLEKIARDVADVAVRK
                     LTAAECPSGKMPVVIGNGFGGVIFHEACGHPLEASSVSKGLSPFAGKVGKQIAHPAVT
                     AVDDGTIVNGWGSNNIDDEGVKTKKNVLIKNGILQSYLVDRFNGRRMGIESNGTGRRQ
                     NYTFEPTSRMSNTYIAAGKYTPEEIISSVKLGLYAKSLGGGQVNPATGDFNFACDEAY
                     IIRDGKVAELVKGASLVGNGADILMKIDMVANDLERAQGMCGADSGSVPVDVGQPTIR
                     VSEITVGGRGGALQ"
     gene            1456..2775
                     /locus_tag="PK460_00825"
     CDS             1456..2775
                     /locus_tag="PK460_00825"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOE53681.1"
                     /translation="MNVKKFFELAKEKGLEAAELVIQKNKGIEMSVYDESVEKYTLAS
                     TINVLARGIYKGKMGFAMSEKDDKTTAQYLVAQIKMSAGVSETEDLSIIFEGSPKYRK
                     KSFFNKELATISESTKLESLFTIEKALKKAHPLITSVSTVQYSEIESDYKLFNSYGLK
                     LTNKQNYFYYGTWVVAKEGDDVKSHVEVFLDNDYSKFDADTFVKKAADGVIEKLGGKP
                     CATKDYPVVFNPKMTASLLAAYLTSASADRVEKNTSLFADKLNERVASSKVTVLDAPL
                     TANLFYRYFDDEGVAKTNKPIIKNGILQTFFHNLSTATKMGVEPTGHGRNTGGRISVG
                     ISNITLKPGRNDFDTLIKDIKEGVFITELMGLHSGLNTTSGDFSLQAAGFMIRDGKVA
                     EPVNLITVAGNLVKLFNDVKAVANENELQLSSYTTSSILVKSLKIAG"
     gene            2898..3812
                     /locus_tag="PK460_00830"
     CDS             2898..3812
                     /locus_tag="PK460_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOE53682.1"
                     /translation="MKKKLLLLLPFLLLAGCKAREKPDIVVTSFVSYDAVTNIVGDKL
                     KVKNIVPWGSELHDFEPTPRDIEDINNAKLFVYTHPALETWVKDLVTNKNSFDMSIHS
                     DVEYACKVKKQEHHDHEHDHDHTLHYWTDPHIYIRVMNDLLTVLIDISPANETYFTEN
                     HNNYTARLLATSNDFRDFLFPPLEKPPLFFAGHNALGYFAAAYKLTIKTISESYKPDV
                     DFLSQEIIDFMYEIKTFDSHYLFIEELVEPRVANLIKNELAKENYAITLLELHGYHNI
                     TAAQAKEGITYADIFAQNVANIKQAFGG"
     gene            3757..4464
                     /locus_tag="PK460_00835"
     CDS             3757..4464
                     /locus_tag="PK460_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOE53683.1"
                     /translation="MPTSSRKMSQTSNKPLAVKLTDVSVNFGDVIALKNINFSINKGM
                     LLYVIGPNGSGKTTFIRLLAKLLEPTHGTIEHSDDRLGYLPQKLNINPNFPITVEEVI
                     YSGLAKQSLLISKGDRELIKSWLKKMQIPHLLKKPMAHLSGGQQQRVYIIRALISNPD
                     FIILDEPTSALDPSFRSFFNDIITALHHEGKTIIFVTHELHGALCDCASVLYIDQEIR
                     FFGGLQEYFEKEKNIHV"
     gene            4457..5296
                     /locus_tag="PK460_00840"
     CDS             4457..5296
                     /locus_tag="PK460_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOE53684.1"
                     /translation="MFSFLQHTFMVYALLLALLIALAAALLSPFLVLSKQSLIADGMA
                     HIAFTGVVIGLLFSNEPLYIALPFALLASIAITFLTERANLDNDAAIGVVSVFALALG
                     LIIISKSDGFNRSVESLLVGSLLTVTVPEIIGASVLALLVICFILIFYRKLIITTFDV
                     THARFAGVNTMFLKYALSALTSIFVVVGVRAIGALLISAFILFPTLISRQLSKSFNMT
                     LWGGVIISLIATFIGITVSYHFTLPTGPAIIMVFTTFLIVAYIIRIIIAKRRLKDAPN
                     ARS"
     gene            5274..5651
                     /locus_tag="PK460_00845"
     CDS             5274..5651
                     /locus_tag="PK460_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOE53685.1"
                     /translation="MRLTPARKLVLNLFKTKSSLLCAEDIERLLKNESIFLSTIYRAL
                     EYFEKENVLGKSTINKTAYYYLIDGKHHHYMICTTCLARFPIDCKLDSMINKNMKKHN
                     FVPTSHDLTIYGYCENCAKLQNN"
     gene            complement(5696..6550)
                     /locus_tag="PK460_00850"
     CDS             complement(5696..6550)
                     /locus_tag="PK460_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOE53686.1"
                     /translation="MPKFKIVVDSTIDLSPEQYKKLGAIVLPLNVHIGEETFQDGVDI
                     NADKLYEKVAETKTAPKTSAVAPGVFKEVFEKLKAEGYTGALFVGIGESLSATAQSVR
                     LAAEEVKDFKVRVVDSHSLSTGSGLLALRAYDLLNEGKTLDETADYLEKIAPKSRAQF
                     IVDNLEMLAAGGRVTGIKLFFGRILRAHPFLQVNNDKLEVVATPKGKSERALDLMLQV
                     AREEISAGLESPRVFITHSHGGERIDYLYDRLKEFVDPKNILISEAGAVISSHCGAGT
                     IGILYIRK"
     gene            6726..7235
                     /gene="mscL"
                     /locus_tag="PK460_00855"
     CDS             6726..7235
                     /gene="mscL"
                     /locus_tag="PK460_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOE53687.1"
                     /translation="MRKFFKEFKAFIARGNVLNLAVGIIIGGAFNAIITSLVNHVIMP
                     PISLLLGNEVSLLKWVIRPEVRDAADNVVTAEIAISWGLFVQAIINFLLIALVLFVIV
                     RVVTRLTEKAEALRAKLLHEEAAAAPEPEPEPEPEPSEEVLLLREIRDSLVAKKQEAK
                     ATKKKEPKK"
     gene            complement(7293..8414)
                     /locus_tag="PK460_00860"
     CDS             complement(7293..8414)
                     /locus_tag="PK460_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53688.1"
                     /translation="MKKEDLLSILVYLVMLIIALFIGLRIIQPALDALDLVTDLQRYG
                     FAILTILVGIIINVILFELGHVFGALLGGYSVISVNILGLAYYKTKSGWKFSFRRYEG
                     LTGETKIIAKSDKTKPRLYLFGPTIMVLLEFVVAIVIFLLTKYNQPIHHQVLIVAGIG
                     AMLLVYNIMPFKLDNFTDGYYIVLLGKKVNVEAYNELIRIESLVYNNEKVTDIKEFDE
                     VTTMTARLSLYRLYQLIDEKAWDKALSLIDDLEKNASKVEHEFIARIRAQKLYIYLLT
                     KASEAASAYWFDELSAADRKFISNDLTIETMRVYLLYSGLVTKSQSECAFVLSRAPKA
                     LRARINDFRKEEEIALFNEAYEKIVALPGNENLKLPVLK"
     gene            complement(8433..9605)
                     /locus_tag="PK460_00865"
     CDS             complement(8433..9605)
                     /locus_tag="PK460_00865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE53689.1"
                     /translation="MHSFNLKIQTELIFGNGRHTEVGSIIKRYGKKNVIILYGQNHAV
                     RSGLLEAVTNALDAENIDYVLYGGITPNPEVFYVREAKAIAKEYAVDFILAIGGGSVI
                     DVAKSLSVNFYYEGDALDFSKRIVTPKKALPLGVILTHASAGSEMSASCVISDSTNNF
                     KQGFRNDLVRPLFAITNPELTYSVSMYQTAVGIVDTFMHSLERYFNKSDKIELADRFA
                     EAIFVTLRECAETLIADPLDETARANLMLASTFSHNDVTSMGKKANLVVHTLEHALSA
                     LYPSIAHGHGLAVLYPAWLFVYFEELKEKLDRFARVVFNLHHEDIDTNAKEGILALKE
                     LFMRLGLTTTLSSLGVKREDIPTLADIVTENGTKELYHDQKNLTRDDIIKLYESCF"
     gene            complement(9598..10143)
                     /locus_tag="PK460_00870"
     CDS             complement(9598..10143)
                     /locus_tag="PK460_00870"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOE53690.1"
                     /translation="MKTKARLGNSLKKFMATMPLDTISVINITKDCKVNRQTFYYHFR
                     NIYDLLTWVFLNEEIEKPSNNELEEAIFIVLRYVKHNEAMIENTLASAAKDLVREFLF
                     NYLYNVCLNYIDKKDPFEKLTLNERKLLARVYGAGIASSIISWIEGEYLFPEHEFVMN
                     LVTFFNDFPLDAINYKGHGYA"
     gene            complement(10133..11050)
                     /locus_tag="PK460_00875"
     CDS             complement(10133..11050)
                     /locus_tag="PK460_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOE53691.1"
                     /translation="MKIRISQEVAYALANNLPVVALESTIISHGMPYPQNVEIALKVE
                     EIVRANGALPATIAIIGGDVVVGLDEDEIEELGQRKDVIKVSRRDLAYVMATKKWGAT
                     TVAATMVIAAKVGIKVFVTGGVGGVHRGATETLDISSDLDELARTNIAVVCAGPKAIL
                     DIEKTLEYLETKGVPVLGYKTDKMPVFYTTSPSLNVLYRVDSSSEIASIVNASHALGL
                     ESGTLVVNPIPKEEALPYKDIEKVIDEALEEMKKAGVKGKEETPYLLSKLNELTKGKS
                     QAANEALVINNAKLGASIAKHICRELYED"
     gene            complement(11054..13540)
                     /locus_tag="PK460_00880"
     CDS             complement(11054..13540)
                     /locus_tag="PK460_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOE53692.1"
                     /translation="MEEFVETKKKRPGTATTIIKKSEIKVDEVIRYTPKHDEGLSTQQ
                     VNARKVAKMTNEKPNKGVKSIFSILMRNFFSFFNVLLYAIGAALIYIKEWQSLLFLVI
                     LFSNILIGLIQDFRARRTLKKLLILNEQKVTVVRDGIELLIPSEDVVLDDIVKLKLGD
                     QIAADGVVVHGSVGVNESLLTGEPNIIHKAKDDRVLSGSFVTTGTCYYQVDTIGKLGY
                     AQKLQLRAKVFKRPTSELLNSINSLFKVIGFIVIAMALLLVFNAFYQGSTFEDSVRSI
                     AGSLVAMIPSGMYLLTSMTLAVGVIRLGTKNTLVNEMYSIEMLARVNVLCLDKTGTIT
                     DGTMKVNEVVPLKGAKDIDLNIIVASILRATKDENATAIALKNHFKTRKYMEYTKVLP
                     FDSELKCSGAIMEGKTYIIGASSMVLGPKAQKQVEEVERIYLERGLRVLVLASSRKPF
                     IAKKLPIDMTPLALIVIQDNIRAEASEVIKLFQDAGVEVKIISGDDPVSVSEIARQVG
                     INNAENLLDFSKIDDPSHHSLEDFTIYGRVRPEQKEGIVTKLQSDGKVVAMVGDGVND
                     VLALKAAQCSIAMGAGSPAARGVSHLVLLDNNFDVLPSIVDEGRRAINNLQKTWSLFL
                     VKTIFAIIFSFMFVTAGLLGPKGSGIRYPFEPKHLYLWELLSLGIPAFFLSMQPNKER
                     VRGTFMDNVISRSVPAGLLMVAGAFLLYISKNFGEMFGAEISDTSMEMITMSILTITF
                     LSFVILYRNSLPFNRYRLTLFLVLSLLAIGVIILDNTVGLTFTEKYFLEINISSLEQI
                     HYAILTPINLILTIMYILVDNKIVKKLRRE"
     gene            complement(13560..16112)
                     /locus_tag="PK460_00885"
     CDS             complement(13560..16112)
                     /locus_tag="PK460_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE53693.1"
                     /translation="MLVLKDIYKDYYVAKKPLHVLKGLNLTFPKQQFVTILGPSGCGK
                     TTLLNLIGGLDKYTSGDLIIEGKSTKEFKDRDWDAYRNHRVGFVFQSYNLIPHLSVIE
                     NVEISLTLSGMGSSERREKAKQALLSVDLESELYKRPNQLSGGQLQRVAIARALVNDP
                     SIILADEPTGALDSETSEQVMKILKEISKTRLVVMVTHNEDIAKRYSDRVIRMLDGRI
                     TSDTDEAQSKKKRKVKEPTETEKNKGTSMSFLTALKNSYKNILTKKGRTVFTALAGSL
                     GIIGIGLVAAVSNGFQNYINRLERETMANYPIAVFAQTMDMQAGIKKPDKNTEYPDGE
                     EIVVYNENDTETTRITITNNRITSEFLDLVDGLKEDGLAASTMVNYPTQMQILAKPPT
                     DNARTVQWPYYVSVTPLPFGVFTQLPGDEAYITEFYDVIGKDSKFATERNEIMFVLDK
                     YNRISVQALERMGIVDNSANVQKLNFDDIIGREFKMVHNDDIYKKALGNIDDYVVLDF
                     MGQPRTIEKYRFQNPQYLYDDPTVGEMLKITGILRPKPGIQFEMLAPGMVYTQALNKY
                     MHETNKHNKVAKAQVNNVVIREANITAENINTAIEFYSATTDWSGVKPVNPTTIRIDK
                     FLSNLKIYGVDLADISKDNAIYETLLKELLNTAYSPVESVAIFAKDGDSKKEIKARIN
                     EYNNTREDEKDKIVIFDAVEFVTDSIATMIDVVSIVLIVFTSISLVVSGFLIGIITYV
                     SVIERTKEIGILRAIGARKKDISRLFNAETFIIGLLAGVIGVGTTYILSIPINVILNN
                     LFPEQNIGQIAFLVPLAALALIVFNILLTVTAGLIPSKMAANKDPVVALRTE"
     gene            complement(16253..17074)
                     /locus_tag="PK460_00890"
     CDS             complement(16253..17074)
                     /locus_tag="PK460_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53694.1"
                     /translation="MRKRILVIISLLFSVVLNGCANKEKEKSELEKFLIKYKEVYPDG
                     YNFQTGFYSITSFKKSKVENTDYFHIETYNIKAQLQSKPYGTLWNFEANMMQLNHSLV
                     GGVGTEIPNENFENILWDGEYLHKTIKYNNGYVPKETWTEKTYGTNQIETLQINTEID
                     KIDYIISTLETKLTDLEKHVVNKSNVNFKNNSLIVSTFFSKLETYKIIRIDFDENFVI
                     SRYQISYTDLGRVPDELRLRQITQILKPIDAIEVNAQLEYEEELIVEKDIFTIQI"
     gene            complement(17355..17507)
                     /locus_tag="PK460_00895"
     CDS             complement(17355..17507)
                     /locus_tag="PK460_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53695.1"
                     /translation="METLKLKTYIINFPTNGTKCFRYYMTSEAVGSRNKGRISVGTTR
                     IYVDKI"
     gene            complement(17574..18392)
                     /locus_tag="PK460_00900"
     CDS             complement(17574..18392)
                     /locus_tag="PK460_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53696.1"
                     /translation="MQKRILIVISLLISVVLNGCTKPQKEQSELEKFLIKYKEVYPDG
                     YNFQTGFYSITSFTKSKVDSSDYFEIETYNIKAQLQSIPYGVLWNFEANMMQLNRSLV
                     LGAGREGPIENFENILWDGEYLHKTIKCNNYIHKETWTEKTYGTDQVETLLINTKLYT
                     IEYVIRILKTKLSYYEKYVFDKSNVDFINNSLIVSTFFSEFEIYKIIQIDFDENFVIS
                     RYQFSYIDLGRVPDEFRPRQITQTLKPIDAIEVNAQLEYEEELIVEKDIFTIQI"
     gene            complement(18491..18946)
                     /gene="tadA"
                     /locus_tag="PK460_00905"
     CDS             complement(18491..18946)
                     /gene="tadA"
                     /locus_tag="PK460_00905"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOE53697.1"
                     /translation="MNKDEKYMKLALKEAHKAAAIDEVPVGAVIVLNDKVIARGFNKR
                     EINNDPLGHAEMIAIRKASKKLNNWRLVDCELYVTVEPCAMCAGAIMWSRLKRVVYGT
                     GDPKGGAMGEAFNLFDERIVNHIPEIKGGVLAKECKEVIQSFFRNKRKI"
     gene            complement(18951..19047)
                     /gene="ffs"
                     /locus_tag="PK460_00910"
     ncRNA           complement(18951..19047)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK460_00910"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(19057..20334)
                     /gene="serS"
                     /locus_tag="PK460_00915"
     CDS             complement(19057..20334)
                     /gene="serS"
                     /locus_tag="PK460_00915"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOE53698.1"
                     /translation="MLDINLIRNEAEKVKSLLFKKGWDFDPEPILSRDEKRRKLRVEV
                     EEAKAKLNKLSASVPVVKKAGGDIQAIFAEVKDLKAENEAKEKELKKLDEEVFNLLAE
                     LPNLPDEDLLAGGKENNKVVYTFGKMPKFTFPIKDHVELCESLGLIDYKRAAKISGSG
                     TWIYTKAGALLEWGLLNFFVSEHLKAGYDMILPPHLLNYESGFTAGQFPKFKEDVFWL
                     EGTEPRKFLLPTSETALVNFYRDEILKEDELPKKYFAYTPCYRSEAGSYRTEERGMIR
                     GYQFNKVEIFQYTKEQDSSKAFEEMVAHAAGLVEKLGLHFQISKLAAGDCSHSMARTY
                     DIEVYIPSMGIYKEVSSVSNARDYQARRGMARYRDKETNAVKYLHTLNGSGLATSRLI
                     PAIVEQFQQADGTVLVPEVLQAFVGTKVLHPDW"
     gene            complement(20590..21591)
                     /locus_tag="PK460_00920"
     CDS             complement(20590..21591)
                     /locus_tag="PK460_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53699.1"
                     /translation="MKILISFPHKKIDVFFEGAMLEYQIKKACQRDDVTLVTKFSPDI
                     DIANFINLNSQSSKVIRQSIAASIPTLLWMFFANNDTYARVIETRKDGELYIPLSRLE
                     LINMMDGIVVPAQEARVILRKLGVKIPIFVIYGAVDVRRIDELKTSKTDVFRRYFRIN
                     DDQKYAITVMNIKASEHIDQLNDLAAAVPNYNFYVFISGGTSFIDRLRLKAMDKLTSP
                     NLIVTKLVPEDVYRMGVIGASYFIDLGYDKMNVMTLFEPMYLKVPLILRKNVIFKEIV
                     DKTKAYIVNDYNGAAYLIRTEAETDKITEKAYEYTQNYNAALFTEAIYNLFYKIYTR"
     gene            complement(21594..24122)
                     /gene="gyrA"
                     /locus_tag="PK460_00925"
     CDS             complement(21594..24122)
                     /gene="gyrA"
                     /locus_tag="PK460_00925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOE53700.1"
                     /translation="MVEDNIKDMLEEEEKHELEGIDTGIHPVDVVDEVRKSFLDYAMS
                     VIVSRAIPDVRDGFKPVHRRIVYGMYESGMTPDKPFKKSARIVGDVMGKYHPHGDQAI
                     YSTLVRMAQPFSLRYPLVDGHGNFGSIDGDEAAAMRYTEARMEKLATEMVRDINLNTV
                     DFGGNYDGSEKEPEVLPSRFPNLIVNGSDGIAVGMATYMVPHNLSETIDAINLVAKNP
                     DVTPREIFENGMLGPDFPTGASILGRKGILDYFETGEGSIIVRAKTHFETKGDRTWIV
                     IDEMPYKLNKATTIEAIALLVRNKEIDGITDIRDESNKEGIRVVIELRRDVVPEVVLN
                     NLLKQTRLQTSYKITNLCLVNKAPRILNMKELIVEYLNFQVEIIRRRTEHQLSQAEAR
                     DHIVEGLLKATDNIDEIVSIIKNSESGEEASQKLMERFLFSEIQTREVLGMTLRRLTG
                     LEIDKLNKEREQLLANIERYRLILSARDHMVDVVLEELEEIKAKYGDERRTEISDDLA
                     DIDNEDLIPVEDIVVMLTNQGYIKRVDPEEFRTIKRGGVGVKGMRTKEEEIIDIILHT
                     NTHTDVLFFTSKGKVYRKRGYQIPQYRREGKGLPVINLLNIEKEEAVRAIVSTDTYGE
                     DHYLLYVTRNGVTKRTTLAEFSRINVSGKIAIDLREGDELMDVKITDGTAIIGIGSSN
                     GKMASFYETDVRAMGRTAAGVRGIKLDEGAYVVGVTTSLEGALIFALTTKGYGKLTPA
                     NDYRLTKRGSKGVITIKESERTGTLAGIRAIKGDEDLFVTTASGMVVRTNFKEISTTS
                     RNTMGVRVIRLRPDDVVCSFAVVEKGIAFDEDDHEGEGTPSHEE"
     gene            complement(24133..26073)
                     /gene="gyrB"
                     /locus_tag="PK460_00930"
     CDS             complement(24133..26073)
                     /gene="gyrB"
                     /locus_tag="PK460_00930"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOE53701.1"
                     /translation="MRARESYTAEDIQVLEGLQAVRQRPGMYIGTTGLPGLHHLVWEI
                     VDNAVDEALAGFCTTIDVSINKDGSVTVADNGRGMPVGIVSQTGKTAVETIFTVLHAG
                     GKFGKGAYQVAGGLHGVGAAVVNALSERVEVNVYKDGDEYYLAFNNDGHTEGELKKVG
                     PTNKRGTRVTFKPDPLIFVESVEFDYKTISTRLRQMAYLNRGIRINVEDRRGEELKRE
                     EFYYEGGIAEYVQHINKNRTPITQDVIFVEGKGDVEINGQKSHILVEVAIQYTTEYKE
                     TIFSFANNIQTGDGGTHEQGFRLAVGRVFNNYAKEAKIHKNEDDRFTYEDVVEGMTAI
                     ISVKHPNPQFEGQTKGKLGNTDTRAVVSQVLGEKLEEYLLENPNEAKLILEKISLSSK
                     ARRAATLAREGVRRKDGIEFTTLPGKLADCSSRNPEESELFIVEGNSAGGSAKNGRNR
                     HTQAILPLRGKILNVQKAQETRIYENAEIGNMIQAIGAGIAEEFDITKARYHKIIIMT
                     DADVDGSHIRVLLLTFFNRFMRPLIQNGYVYIAQPPLYKVDYRGKQYYCYNDEQLEKL
                     KVDLDLRPGYPFQRYKGLGEMDAHQLWETTMDPANRKLLRVHIEDAIEADQVFEDLMG
                     EKVEPRKEFINENALFVKNLDV"
     gene            complement(26133..27200)
                     /gene="recF"
                     /locus_tag="PK460_00935"
     CDS             complement(26133..27200)
                     /gene="recF"
                     /locus_tag="PK460_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOE53702.1"
                     /translation="MLISDLELLNYRNYPKLNVIFTSGLNVIVGDNGVGKTNVVEAIH
                     LFAFAKSFRTNDTKALIKHDTAKAIVSGTIHIPSRTDISIELTNRSKRILINGKVLPK
                     VSQLSKYVRTTTFEPEDVLFFDASPAKRRKFLDANLSSADEVYLATLVRYEKLLKERN
                     VLLKTDDNDEALDLIDKLFIELAKTIVEKRTRFIRELNKVVNEVLLKLSDEHLSVRLT
                     YESSLGSVQNIERNGLKRLAEVRAKENILESTTLGPHRDDLIATINGHGVKEHASQGQ
                     KRLIVIALALAPYFLEKDLHKKPIIILDDVLSELDKRHQERLIELLLTCTQAFITATH
                     YDNHAHAIYEVTSDRELRRIT"
     gene            complement(27190..27393)
                     /locus_tag="PK460_00940"
     CDS             complement(27190..27393)
                     /locus_tag="PK460_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOE53703.1"
                     /translation="METKPYITLQQALKKYNLVSTGGEAKFVIQNGEVKVDGILETRR
                     GRKLYGGEIITYAAQKVVVKDVD"
     gene            complement(27562..28695)
                     /gene="dnaN"
                     /locus_tag="PK460_00945"
     CDS             complement(27562..28695)
                     /gene="dnaN"
                     /locus_tag="PK460_00945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOE53704.1"
                     /translation="MKFKINREQFLKGLSIASRPTQAKSPIPILGNILLSLDETGLTL
                     LGSSNELAIRTHIPMRVNDLEVIRMFLPGETLISSKTLNESIRKMEGNEIDVEIIDRT
                     IAQISDGRTEYRLGAIDSKDYPDIDLSQDGTVFEIGAKDFIKLVDQTSFAASVREQRP
                     ILTALNLNAVNGTLTAVATDSARLANKVVTLKDNVSFVANVPARIMNEVAKLLETMVG
                     IETIEVSASANKINFMFDDTLVVSRLVAGEYPPTRSIIPKTHASFLEANANELLKAMD
                     RVSILYAAEKDNIVKLSLSPESVVVSARSTFSAESAREEVALCKYVGEPLEISFNYTL
                     VSSAIKATGAEDVTIGFNGGHKPFSIRVATDDSLVLIVTPVRT"
     gene            complement(28824..30179)
                     /gene="dnaA"
                     /locus_tag="PK460_00950"
     CDS             complement(28824..30179)
                     /gene="dnaA"
                     /locus_tag="PK460_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOE53705.1"
                     /translation="MNMSISEITQIWDRTLIKLQEKIQDRRVYGAFFPDTYIHSINNG
                     IITVIANSSLAKSMLNTNYRDVIEETVGEITQSNYQISFIEKSEIPRVSEIISEKKET
                     FFKNAKVNSNLTFDNFVVGSSSNLEAYQAAVVVANSPGKLYNPLFLYSASGLGKTHLL
                     HAIGNHVKQNSPNLKVLYITADEFTDEFIRFVRDNPGGSLKDFFRTVDVLLMDDVQFL
                     ADKKSTSETFFHLFNLLVGSGKQIVLTSDRHPSELKGIEERLVTRFNSGLQISISAPD
                     LETRKDILRMKISLNNLDIEDFDEEVITFFAENFTNNVRELEGALNRLLFYVVSIAPT
                     KHVTMEVALNSVQSLINVKDAVTKLSETKIINVVADYYNLTPALIIGRNRTSQVALAR
                     HIGMYLTREILDLPFARIGKAFGGKDHSTVINAITKVEKQLKEDPALIQAINEIKERL
                     Q"
     gene            30686..30820
                     /gene="rpmH"
                     /locus_tag="PK460_00955"
     CDS             30686..30820
                     /gene="rpmH"
                     /locus_tag="PK460_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOE53706.1"
                     /translation="MKRTYQPSKLRRARLHGFRARMATPGGRRVLRNRRAKGRESLTV
                     "
     gene            30844..31167
                     /gene="rnpA"
                     /locus_tag="PK460_00960"
     CDS             30844..31167
                     /gene="rnpA"
                     /locus_tag="PK460_00960"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOE53707.1"
                     /translation="MKREHRILKNEQFQNIIKNGKLTKTPLCYGYFASNELETLRVGV
                     AVSKKIGNAVTRNKIKRQIRAVVRPYVKTHTGDFIIVAKKEINDSSFGDLVDAIAKIF
                     DSNGD"
     gene            31169..32548
                     /locus_tag="PK460_00965"
     CDS             31169..32548
                     /locus_tag="PK460_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOE53708.1"
                     /translation="MKKNKKRIIKLGAIVAGLFVLSSCTASFCSAKDVGYMLYAQDSG
                     LVTEVVEGTETKVHNKVLHKIILDAQSQGYATPSLEYYEKLDQKVLDYAILNFDNSEN
                     LEGAALNAAALKQNGYLKFLGTKGATVVTADGNELWVNWTIWNKEIGAEIGYENVPDR
                     DFANFYKTQIYNKIKANRACIALYDGEYGPEDSKIPVEAKTWKGAWKKGFIEGLIVYP
                     VAALLEFLTKSFGAGGWGQIWAILVTTIIVRGLLVLMTLKQTVGAQKMQALQPELAKI
                     QQKYPNSDTNQYERQALAQAQMALYKKHGINPLGSLLVMFIQFPIFIGVWGAMTGSAS
                     LASDSVLGLNLSAQLGQSMINGWFKGGWWTAWVLFILMTAAQFVSTKLSTWLNKSKTK
                     EIGKTTANPAADKQQRQSKMMMNIMFIMIVFMSFTLPAAMGVYWFVGAIISIVQTVVV
                     HNVMKGRKQ"
     gene            32548..33189
                     /locus_tag="PK460_00970"
     CDS             32548..33189
                     /locus_tag="PK460_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOE53709.1"
                     /translation="MRIFEAKNLDEVLALASEDIGIQTKDLVYEVLEEKKGLFKSSIK
                     ISVYETTDYIEYAENYLRLVVESLGFTPEFTTSLDGEVIKIEMSTNHNSILIGKGGKT
                     LLALNEIVQVAMTVKFKKRIRVLLDINNYKNDRYRRVTGLARKVAGDVARSKQDATLD
                     PMPSDERRAIHNALSEYRNVTTESIGAGKERQVIIRYKKPEKKEKAPKEPQDE"
     gene            33258..34580
                     /gene="mnmE"
                     /locus_tag="PK460_00975"
     CDS             33258..34580
                     /gene="mnmE"
                     /locus_tag="PK460_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOE53710.1"
                     /translation="MLDTIVALATPPMMGALAIIRLSGPEAFNIVTKSFTKDLTKLEK
                     STMVYGKLFDDEELVDEVIAFCFKGPNSFTGEDVVEISCHGSMLIANEIIELMIKNGA
                     RLAGRGEFTNRAYLNGKLDLVQAEAVNDMITATTKEAKKLSLLSLVGETSKIVYPIRE
                     RIADVMSLIEVNIDYPEYRDIEEASYALVISEVDYINAEIKKLIDQGRQGQIIKEGIK
                     VAIVGAPNVGKSSLLNALLKEDKAIVTDIAGTTRDVVEGEINLHGLRLLLLDTAGIRE
                     STDAIESIGIEKTKKSIADADLVIYVKDAENVSKKDEFTGLIKGKKVITVYNKGDLVS
                     KKDPNRLYISALTNDIGALEKAIAKVVGVDEDSYLRPSLNNARQLALLRKAYGELEIA
                     KQEALDEVAIDLVSVSLQQAFFTIKEILGENVDNDLSKEIFSRFCVGK"
     gene            34583..35350
                     /locus_tag="PK460_00980"
     CDS             34583..35350
                     /locus_tag="PK460_00980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE53711.1"
                     /translation="MFFDTHCHLNDKRYENDIEGYVERAKAVGVNYLLIVAWDVKSSK
                     KAIKIAERFNNVYAAAGIHPVDAVKTPKSDLKELEKLLTHPKVIAVGEIGLDYHWIKD
                     EKERAIEHEFFIAQIELANKYKKPVLIHMRDATSDTYEVLKSNIPRYQGIMHSYSGSK
                     EMALRFIDLGMYISLAGPVTFLNAREPKEVAKVVSEERLLLETDAPYLTPHPYRGKLN
                     ESANIPLIAKTIATIRNVSVEKVAEFTTKNAKTLFKL"
     gene            35347..35886
                     /locus_tag="PK460_00985"
     CDS             35347..35886
                     /locus_tag="PK460_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4093 domain-containing protein"
                     /protein_id="HOE53712.1"
                     /translation="MSSKTLLNCIFVAEGKRDEERLKKLDIPYIVRTDGLNVPRETTK
                     HLQELAKIHEVIILTDPDGPGQKIADKLLQEIPNAKVIKVIKKKAVKKGKVGIEHIPL
                     PLLAELIKPYKQETFTTSSDVTLIKLLDLGLSGPNSQFKKIKLATKYSLLVASLKNML
                     TQIQLLNISYKELKEALSE"
     gene            35879..36664
                     /gene="rsmA"
                     /locus_tag="PK460_00990"
     CDS             35879..36664
                     /gene="rsmA"
                     /locus_tag="PK460_00990"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOE53713.1"
                     /translation="MNKEEIITLLAKENLSPQKRKGQNFLVDENVARKIVSFLEPNYI
                     DVIEVGPGLGALTKELLLKTVNFTAIEIDKGFVRVLRAKYPHLNIIEKDFLEYSVPRE
                     TKAVISNIPYYITTKIIEKVLLEAENLKKFVFMTQIDVKERLLAKSGTKAYSPLRVLL
                     SLLGDVEQKLVVTADKFYPVPHVDSAVFCFTRYKTDIPVREFYSFLNNAFLNRRKTLY
                     NNLKNIYDKEDILTALKKHRINENVRAEALSPNDLLTLFETLR"
     gene            36967..37395
                     /locus_tag="PK460_00995"
     CDS             36967..37395
                     /locus_tag="PK460_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53714.1"
                     /translation="MKNKKKLFWLILPFTLIMFALLFALYPTPWGAKEPYKVFHSPEK
                     HLKEVEKRVNQKIEEREMDYTNLRALPLYDEYNKFSFVLVEFEPQDFMILKVYRTSII
                     SVKRTIYLRDTNNSFGVHTENNNENSQFNPYQNCDTSNNK"
     gene            37894..38745
                     /locus_tag="PK460_01000"
     CDS             37894..38745
                     /locus_tag="PK460_01000"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53715.1"
                     /translation="MIVTANARLSISVETTLNPLTNALEIKSLVLPIEFSDTITIDVF
                     NTAFMDIVHYTNKALHVKEKRIIDEALLKMREQFGYKQNFSVKIHKNIPTGHGLGSGA
                     SDATAVVKAVAHLAKLKPTPEQYYNLAVKIGKDVPYFLVNKPAVYNLNKQEVTPIKFK
                     HNPQVLLIIHPDIYEKENVVKDFLDYGESLQKPLEKVVELAESGSLRELGSVLFNDFS
                     ETIIKHTPALRTVLNDLKQLNVEAYGIAGTGTTIFALSDNRHLLRFISDKYRKQNYQT
                     VMTKVIR"
     gene            38755..40143
                     /gene="glmU"
                     /locus_tag="PK460_01005"
     CDS             38755..40143
                     /gene="glmU"
                     /locus_tag="PK460_01005"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOE53716.1"
                     /translation="MKRNAIILAAGKGTRMKSLNQEISKVGFEILGKPLINWVLDAAK
                     PHVTGRTVVIVGFGGEHTSKLVEGKAEIAWQHEQKGTGHAIIQTAPLLEKEKGDTLIL
                     SGDVPLITEHSISGLLNLHTKEKNDLTILTAKPDNPFGYGRVLRAPNGEVTRIVEQKD
                     LTGNEGDISEVNAGMYVVNNQKLFQELKNLKPNNKQGELYLTDIIGLFKERGYKVGAF
                     VLSDPDEMLGTNDRAQLAEATELLKKRINKAHMLNGVTLEDPNNTYIGPDVTIGQDTI
                     ISPNTTILGNSTIGVANEIGPNTYLKNMKIGDYNSIIYSYLVDSEICNNVTIGPFARM
                     RQKALVKDGSHIGNFVELKNTTFHEGVKAGHLAYMGDAELGAHTNVGAGAITANYDGV
                     NKSKTKVGENVFIGTNTTLVAPLQIGEGSYLAAASIINKDVPKDTLAIARSRQENKEG
                     YAKIIREKQKKK"
     gene            complement(40248..42890)
                     /gene="ppdK"
                     /locus_tag="PK460_01010"
     CDS             complement(40248..42890)
                     /gene="ppdK"
                     /locus_tag="PK460_01010"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOE53717.1"
                     /translation="MAEKRYVFAFKCKEGEKLGRNYLGGKGANLAEMTRIGVPIPQGF
                     TVTTEACNLYYNEGKKLPQFVIDQIYNAVTELEKETGKTFGGKKNPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDEVAEVLANETGNAAFAFDSYRRFILMYFNVVKGQPRAEMEKML
                     EDMKVKAGVKLDSELKAEHLRELVKQFKAHYKKVLGEEFPSDARVQLIEAVTAVFRSW
                     DNERANIYRRMNHIPYEWGTAVNVQSMVYGNKGDTSGTGVAFTRSPSTGDNHIFAEYL
                     PNAQGEDVVAGIRTPLPISWLEENMPAVYKQFVETLDLLEKHYRDMQDIEFTVEDGKL
                     YFLQTRSGKRTAAAALKIVCDLIDEGLITEEEAVLRVDPKALDSLLHKGFDQAVLKTK
                     KVIATGLPASPGAATGRLSFDAADAAARKEKGETVLLVRSETSPEDIAGMVSAAGILT
                     SRGGMTSHAAVVARGMGTTCIVGAHDVIIDEVGHTMKVGDKVYTTKDVISIDGTTGNI
                     YEGEIPLVEPQLTGYFGRFMELADKYRRLEVRVNADTPLDATHGRDFGATGIGLCRTE
                     HMFFDVDRVFFMRKMILSATKEEREAALAQILPYQENDFYEIFKVMKGLPVTVRLLDP
                     PLHEFLPTEEAQIVALAKELKVDVTVLRERIESLSEFNPMMGHRGVRLAVSYPEIAVM
                     QTTAIISAALRAQKDTGAAIHPEIMIPLVLDVKEYKYVEEYVRKTADALIKKSGQKLH
                     YLVGTMIEIPRATLLADQIAKEAEFFSFGTNDLTQMTFGFSRDDATKFIPDYTEARIF
                     EQDPFQTLDTEGVGQLVRLGVERGRKGNPDVHLGICGEHGGDPNSVEFFDSVGLDYVS
                     CSPYRVPIARLAAAQAAIKHKKAK"
BASE COUNT        13331 a         8063 c         7549 g        14041 t
ORIGIN      
        1 aagcgctttt ccccatattt ccttcatatt tttgttatca tttttaatta ggaggaagta
       61 cattatgatt acaaaaaaac ttgccctcga agtattaaat gtagcgctta gtaccggtgg
      121 agatttcgct gaaatatttt tagaagaaac ttacagcggt aactacactg tagaaaacgg
      181 taaaacgcaa gcgcctgcta cttcgttaac ttatggggca ggcattcgta ttttaaaagg
      241 attacaaagt gtttatggtc acacaagtga cttaagcaaa aaaggtttat taaatcttgc
      301 cactgcgtta agcgctggct ttgatggtca aagagtttta agtgttgaaa aacttaaaaa
      361 tgtaagggtt cgtaaaaatc acgagttaga aaaaagtctt gaattagtgg atattaatga
      421 aattgttgag aagttaaaaa tcactaataa agtaatgaaa gattatgacg aaaagattgt
      481 acgcgctatc agtgtattat taactgatgt tcgtaatatc actattttta atagtgaagg
      541 taatgcattt actgataagc aagagcgcgt aagattggtg tttcaagcaa tagcaatggc
      601 accaggcgcc tttgaatata gctacaatgg accaggagca cacaaaggct ttgaatatgt
      661 tgaaggttta gatttagaaa agattgcgcg tgatgttgct gatgttgctg tccgtaaatt
      721 aaccgcagcg gaatgtccaa gcgggaaaat gccggttgtg attggtaacg gctttggtgg
      781 agttattttt cacgaagcat gtggtcaccc gcttgaagca agttctgtta gtaagggatt
      841 atcacctttt gccggtaaag tcggaaagca aattgctcac ccagcggtta ccgcagtcga
      901 tgacggcacg attgtaaacg gctggggatc aaacaatatt gatgacgaag gcgtaaaaac
      961 aaagaaaaat gttttaatta aaaacggcat tttacaaagt tatcttgttg atcgttttaa
     1021 tggacggcgg atgggaattg aaagcaatgg tacaggccgg cgtcaaaatt atacctttga
     1081 accaacaagc cggatgtcaa atacatacat tgccgctggt aaatatacac ctgaagaaat
     1141 cattagcagt gtaaaacttg gattatatgc aaaatcactt ggtggtggac aagttaatcc
     1201 agcaactggt gactttaatt ttgcgtgtga tgaagcttat attattcgtg atggtaaagt
     1261 tgccgaatta gttaaaggtg cttcacttgt tggtaatggc gctgatatat taatgaaaat
     1321 tgatatggtc gctaatgacc ttgaacgcgc tcaaggcatg tgcggtgccg atagcgggag
     1381 tgttccagtt gatgttggcc aaccaacaat tagagttagt gaaattaccg ttggtggtag
     1441 aggaggggca ttacaatgaa cgtgaagaaa ttctttgaat tagcaaaaga aaaaggcctt
     1501 gaagccgctg aactcgttat tcaaaaaaat aaaggtattg agatgagtgt gtatgatgaa
     1561 agtgttgaaa aatacacctt agcgtccaca attaacgttc tagcgcgcgg tatatataaa
     1621 ggtaaaatgg gttttgcaat gagtgaaaaa gacgataaaa caactgccca atacttagtt
     1681 gcccaaatta agatgagtgc cggagtttca gaaacagaag acttaagtat tatttttgaa
     1741 ggtagtccta aatatcgtaa aaaatcattc tttaataaag aacttgccac tattagtgaa
     1801 agcacaaaac ttgaatcgct ctttaccatt gaaaaagcgt taaaaaaagc gcacccgctt
     1861 attacttctg tttcaactgt tcaatattcc gagattgaaa gtgattataa attatttaat
     1921 tcatatggtt taaaactcac aaataaacaa aactacttct attatggaac atgggttgtt
     1981 gctaaagaag gcgatgatgt taaatcgcat gtcgaagttt tccttgataa tgattatagt
     2041 aaatttgacg ctgacacatt tgtgaagaag gcggctgatg gagttataga aaaacttggt
     2101 ggtaaaccat gtgctactaa ggattatccc gttgtattta acccaaaaat gaccgctagt
     2161 ttacttgcag cttacttaac aagcgcaagt gctgatcgtg ttgaaaagaa cacttcttta
     2221 tttgccgaca aactaaatga aagagtagca agttcaaaag taactgttct tgatgcaccg
     2281 ttaacagcta atctcttcta tcgttatttt gatgacgagg gcgttgctaa aacaaataaa
     2341 ccaattatta aaaatggtat cttacaaaca ttcttccata acttaagcac cgctactaaa
     2401 atgggtgttg aacctaccgg tcacggccgt aacacgggcg gaagaattag tgtcggtatt
     2461 agtaatatca ccttaaaacc tgggcgtaac gactttgata ctttaattaa agatattaaa
     2521 gaaggtgtat ttatcactga acttatgggt ctccactcgg gactcaacac aacaagtggt
     2581 gactttagtt tacaagcagc agggtttatg attcgtgacg gtaaagttgc tgaacctgtt
     2641 aacttaatta ccgttgctgg taacttagtt aaactcttta acgatgttaa ggcggtcgct
     2701 aatgaaaatg aattacaact tagtagctac actacttcaa gtattcttgt taaatcatta
     2761 aaaattgctg gctagtaata aagtatcttt atctaaattt aagaggcgtc ctttgtggcg
     2821 cctttgcttt ttattagcga taacgctaag ctgtgtgtta taattctttt tgcgaatcat
     2881 tcgcaaattg aaggattatg aaaaagaaat tattattact attaccattc ttacttttag
     2941 cgggttgtaa ggcgcgcgaa aaaccagata ttgttgttac ttcgtttgta agttacgatg
     3001 ctgtaactaa cattgttggt gacaaactaa aagttaaaaa cattgtccct tggggcagtg
     3061 aacttcatga ctttgaacct acaccacgtg atattgaaga tattaataac gctaagctat
     3121 ttgtctatac tcatcctgcg cttgaaacat gggttaaaga ccttgtcact aataaaaata
     3181 gttttgatat gagtattcat tctgatgttg aatatgcttg caaagtaaaa aaacaagaac
     3241 atcacgatca cgaacacgac catgatcaca cacttcatta ctggacagat ccacatatat
     3301 acatccgtgt tatgaatgat cttttaactg tgcttattga catttcgccg gcaaatgaga
     3361 cctattttac agaaaatcat aataattaca cagcgcgatt attagccaca agtaatgatt
     3421 ttcgtgactt tttgtttcca ccgttagaaa aaccaccgct tttctttgcg gggcataatg
     3481 cgcttggtta ttttgctgct gcttataaac taactattaa gacaattagc gaaagttata
     3541 aaccagatgt tgatttttta tcacaagaaa ttattgattt tatgtatgaa attaaaacat
     3601 ttgattcgca ttacttattc atcgaagaat tagtggaacc gcgtgtcgct aatttaatta
     3661 aaaatgaatt agcaaaggaa aattatgcga ttactctttt agaacttcat gggtatcaca
     3721 atattactgc agcacaagca aaggagggta taacatatgc cgacatcttc gcgcaaaatg
     3781 tcgcaaacat caaacaagcc tttggcggtt aaactcactg atgtaagtgt aaattttggt
     3841 gatgtcatag cgcttaaaaa catcaacttt tcaattaata aagggatgct cctttatgtg
     3901 attggtccta acggatcagg taaaacaact tttataaggt tgcttgctaa attgttagag
     3961 ccgacgcatg gcactattga acatagtgat gatcgtttag gttatttacc gcaaaaatta
     4021 aatattaacc ctaatttccc aatcacggtt gaagaagtaa tttatagcgg ccttgctaaa
     4081 cagtcactac tgattagtaa aggtgatcga gaactaataa aatcatggct taaaaagatg
     4141 caaatcccgc acttgcttaa aaaaccaatg gcccatcttt ctggcggtca acaacaacgc
     4201 gtctatatta ttcgcgcgct tattagtaat cctgatttta ttattttaga tgaacccact
     4261 tctgcactcg acccgtcctt tcgcagtttc tttaacgaca taattacagc attacatcac
     4321 gaaggaaaaa caattatttt tgtcacacat gaacttcatg gtgcgctttg tgactgcgct
     4381 agtgtgttat atattgatca agaaatacgt ttctttggcg gcttacaaga gtattttgaa
     4441 aaggagaaaa atatccatgt ttagtttctt acaacacact tttatggttt atgctctttt
     4501 gttagcgctg cttattgccc tagcggccgc tcttttaagc ccgtttcttg ttttatctaa
     4561 acaatcttta attgccgacg gcatggcgca tattgccttt accggcgttg ttattggatt
     4621 actatttagt aatgaacccc tttatattgc cttacctttt gcgcttttag cctcaattgc
     4681 cattaccttt ttaacagagc gcgctaatct agataatgat gcagcaattg gtgttgttag
     4741 cgtctttgct ttagcgcttg gtttaattat tattagtaaa agcgatggtt ttaatcgctc
     4801 tgtggaatcg ctattagttg gtagtttatt aacagttact gttcctgaaa ttattggtgc
     4861 ttcagtttta gctttgctcg ttatttgttt tatcttaata ttctatcgta agcttataat
     4921 tacgactttt gatgtaactc atgcgcgttt tgctggggtt aacactatgt ttcttaaata
     4981 tgccctttcc gcacttactt ctatttttgt tgttgtcggt gtccgcgcga ttggggcact
     5041 actaatctct gcatttattt tgtttccaac attaatctct agacaattat ctaaaagttt
     5101 taatatgact ttgtggggcg gcgttattat ttcactaatc gctactttta ttgggattac
     5161 tgtttcttat cactttactt tgccgacagg acccgcaatc attatggtat ttactacatt
     5221 tctcatagta gcgtatatta ttcgtattat cattgctaaa aggagactta aagatgcgcc
     5281 taacgcccgc tcgtaaactt gtgcttaatt tatttaaaac aaaatcttca ttactttgtg
     5341 ccgaagacat tgaacgatta ttaaaaaatg aatctatttt tctttcaaca atctatcgtg
     5401 ctttagaata ttttgaaaaa gaaaatgttc taggcaagag cactattaat aaaacagctt
     5461 actattactt aatagacggt aaacatcatc attacatgat ttgcactact tgtcttgcgc
     5521 gattccctat tgattgtaaa ttagatagta tgattaacaa aaatatgaag aaacataatt
     5581 ttgttcccac aagtcacgat ctaacaattt acgggtattg tgaaaattgt gcaaagttgc
     5641 aaaacaacta aaaaatgccg cgattaagcg gcatttcatt agtaattaat tattgttatt
     5701 tacgaatata taaaatacca attgtgcctg cgccacaatg acttgaaata acggcgcctg
     5761 cttcactaat taaaatattt ttgggatcga caaattcttt tagtcggtcg taaagataat
     5821 caatgcgttc accgccgtgt gagtgcgtaa taaagacgcg tggtgattct aagccagcac
     5881 taatttcttc gcgtgctact tgtagcatta ggtctaatgc tctttcactt ttaccttttg
     5941 gtgtcgctac tacttctaac ttgtcattat taacttgtaa gaatgggtgg gcacgtagta
     6001 tacgaccaaa gaataactta atgcctgtta ctcttccacc agcagcgagc atttctaaat
     6061 tatcaacaat gaattgcgct cttgatttag gcgcgatttt ttctaagtaa tcagcggttt
     6121 catcaagtgt tttgccttcg ttaagaaggt cataagcgcg tagcgctaat aacccgcttc
     6181 ctgttgaaag tgagtgtgaa tcgacgactc ggactttaaa gtctttaact tcctctgctg
     6241 ctaaacgcac actttgggct gttgcactta gggattcacc aatccccaca aataaagcgc
     6301 ctgtataacc ttcggctttt aatttttcaa acacttcttt aaatactcca ggggcaacag
     6361 cgctcgtttt tggtgctgtt ttcgtttctg ctactttttc ataaagttta tcagcgttaa
     6421 tatcgacgcc gtcttggaaa gtttcttcac caatatgaac gtttaatggt agaacaatcg
     6481 ccccaagctt tttatattgt tcaggactaa gatcaattgt tgaatcaacg actattttaa
     6541 atttaggcat attttctcct ttttgtgatt tgctcacaac tctttaatca tcacttttaa
     6601 gtgacttcac tatcaataat atcttatttt attttatttt gcatatttaa agtactacgg
     6661 tcttatttgt tttactactt acttttagct ttatgctaaa attgaaagca gaaaggaaat
     6721 aaattatgag aaagtttttt aaagaattta aagcgttcat tgcccgcggt aatgtcttaa
     6781 atcttgctgt tggtattatc atcggtggcg cttttaacgc aattattact agtttagtaa
     6841 atcatgtaat tatgccgcct atctcgttat tacttggtaa tgaggtttca ctattaaaat
     6901 gggttattag gccagaagtt cgtgacgctg ctgacaatgt tgtgactgca gaaattgcta
     6961 tttcatgggg tttatttgtc caagcaatta ttaacttctt attaattgct cttgttttat
     7021 ttgtgattgt tcgtgtcgta actagactta ctgaaaaagc agaagcttta cgcgctaaac
     7081 ttcttcacga agaagctgca gctgctccag agccagagcc agagccagaa ccagaaccaa
     7141 gcgaagaagt attattactt cgtgaaattc gtgactcatt agtggccaaa aaacaagaag
     7201 caaaagcaac taaaaagaaa gaacctaaaa agtagtaaaa gaaaaacacc agtttctagt
     7261 ttctaagtaa acatttactg gtgtttttta ttttatttaa gtactggaag ttttagattt
     7321 tcattaccag gcaaagccac aattttttca tacgcttcat taaaaagcgc tatttcttct
     7381 tctttacgga aatcgtttat tctagcgcgc agtgctttag gagcacgact taaaacaaag
     7441 gcgcattcac tttggctctt agtaactaac ccactataga gaagatacac acgcattgtt
     7501 tcaattgtta agtcgttact aataaactta cgatctgcgg cgcttagttc atcaaaccaa
     7561 taagcacttg ctgcttcact agcttttgtt aataagtaaa tataaagttt ttgcgctcta
     7621 attctcgcaa taaattcatg ttcaacttta ctcgcattct tttctaagtc atcaattaat
     7681 gataatgcct tgtcccacgc tttttcatca attaattgat ataagcgata taaacttaaa
     7741 cgtgctgtca ttgttgtgac ttcgtcaaac tctttaatgt ccgtaacttt ttcattgtta
     7801 tacacgagcg attcaattct aattaattcg ttatacgctt caacatttac ttttttgcca
     7861 agaagcacaa tgtaatatcc gtctgtgaag ttatcaagtt taaaaggcat gatgttatag
     7921 actagaagca ttgcaccaat ccccgcaacg attaacactt ggtgatgaat aggttgatta
     7981 tactttgtta ataagaaaat aacaattgca accacaaatt ccaaaagcac cattattgtt
     8041 ggaccaaaca aataaagccg tggttttgtt ttatcgcttt tagcaataat ttttgtttcg
     8101 cctgttaatc cttcataacg tctaaagcta aacttccacc cgctttttgt tttgtaataa
     8161 gctaatccaa ggatgtttac acttatcaca ctatagccac caagaagcgc accaaagaca
     8221 tggcctaact caaataaaat aacattaata ataataccaa caaggattgt taatatcgca
     8281 aatccgtagc gttgtaaatc agtcactaaa tcaagtgcgt caagcgcagg ttgaataatt
     8341 cttaacccaa taaataacgc aataattagc atgacaagat acactaatat gctaagcaaa
     8401 tcttcttttt tcatattcgt cctccaacag ttttaaaaac aactttcata aagtttgatt
     8461 atatcatctc ttgtgagatt tttttggtcg tgatacaact cttttgtgcc gttttccgtt
     8521 acgatatccg ctaaagtggg gatatcttca cgcttaacac ctaaactaga taaagtagtt
     8581 gttaatccta aacgcataaa taattcttta agtgctaaaa tgccttcttt tgcgtttgta
     8641 tcaatgtctt catgatgaag attaaaaaca acacgcgcaa aacgatcgag tttctctttt
     8701 aattcttcaa aataaacaaa gagccatgcg ggatagagca ccgctaaacc gtgcccatga
     8761 gcaatacttg gataaagcgc actaagtgca tgttctaaag tatggacaac taaattagct
     8821 tttttaccca tgcttgttac atcattgtga ctaaaagtgc tcgctaacat taaattagcg
     8881 cgcgccgttt catcaagtgg gtcagcaata agtgtctctg cgcattcccg taaagtaaca
     8941 aatatcgctt ctgcaaagcg gtcggctaac tcaattttgt cgcttttatt aaaatagcgt
     9001 tcaagcgaat gcataaatgt atcaacaatc cccactgctg tttgatacat tgagacacta
     9061 taagttaatt ctggattagt aatagcaaaa agcggtctta ctaaatcgtt tctaaaccct
     9121 tgtttaaaat tattagtgct atcactaatt acacagctcg ccgacatttc actacccgct
     9181 gaagcatggg ttaagattac accaagcggt aatgcttttt taggagtaac tatccgttta
     9241 ctaaaatcta gtgcatcgcc ttcataataa aaattcactg atagtgactt tgcgacatca
     9301 attacactgc caccaccaat tgctaaaata aagtctactg catattcttt tgcaatcgct
     9361 ttggcttcac gcacataaaa gacttccgga ttaggcgtaa tcccgccata aagtacataa
     9421 tcaatatttt cagcatcaag tgcgttagtc actgcttcta aaagcccact tcttactgcg
     9481 tgattttgac catataatat aattacattc tttttgccgt aacgcttaat tatactgcct
     9541 acttcagtat gccggccgtt accaaaaatg agttcagttt gaatctttaa gttaaaacta
     9601 tgcatagccg tgtcccttat aatttatagc gtcaagcggg aagtcattaa aaaatgttac
     9661 taaattcatc acaaattcgt gttcagggaa taagtattcg ccttcaatcc acgaaataat
     9721 gctactagcg atacctgcgc catatactct tgctaaaagt tttcgttcgt ttaatgttaa
     9781 tttttcaaat ggatcttttt tatcaatgta atttaaacac acattatata aataattaaa
     9841 aagaaactct cttaccaaat ctttagcagc gctcgctaaa gtgttttcaa tcattgcttc
     9901 gttatgttta acataacgca gcacaataaa gattgcttct tctaattcat tgttagatgg
     9961 tttttcaatt tcctcattta aaaataccca tgttaataaa tcataaatat tacgaaaatg
    10021 ataatagaaa gtttggcgat taactttaca atctttcgtg atgttaataa cgcttattgt
    10081 atcaagtggc attgttgcca tgaacttttt taaagaattt cctaaacgag ctttagtctt
    10141 catataactc ccggcaaata tgtttagcaa ttgacgctcc taacttagcg ttattaatga
    10201 caagtgcttc gtttgcggct tgactcttac cttttgttaa ttcgtttagt ttggaaagaa
    10261 ggtaaggcgt ttcttcttta cctttaaccc cagctttttt catttcttca agcgcttcat
    10321 caattacttt ttcaatgtct ttataaggca aggcttcttc ttttggaatt gggttaacaa
    10381 caagtgttcc gctttcaagc cctaaggcat gacttgcgtt aacaatcgaa gcaatttcgc
    10441 tagaagaatc aacgcgatat aagacattta atgatggact tgttgtgtaa aatactggca
    10501 ttttgtcggt tttataacct aaaactggaa cacctttagt ttctaaatat tccaatgttt
    10561 tttcaatatc aagaattgct tttggaccag cgcatacgac cgcaatgttt gttctggcaa
    10621 gttcatcaag gtcgctagaa atatctagtg tttcagtcgc accgcgatgc actccgccaa
    10681 cgccaccagt tacaaatact ttaatgccaa ctttagcagc aataaccatg gtcgcagcaa
    10741 cggtcgtcgc accccacttc tttgtggcca taacataggc taggtcacgc cgtgaaactt
    10801 taatgacatc ttttctttgg cctaactctt caatttcatc ttcatccaaa ccaacgacaa
    10861 cgtcaccacc aataatcgct attgttgctg gtagcgcccc attagcgcgc acaatttctt
    10921 caaccttaag cgctatttcc acattttgcg ggtaaggcat cccgtgacta ataatcgttg
    10981 attctagcgc tacaactggt aagttgttcg ctaaagcgta ggctacttct tggctaatcc
    11041 gtattttcat aaattattct ctccttaatt tctttacaat tttattatcc actaatatgt
    11101 acattattgt aagtattaaa tttattggag ttaatattgc atagtgaatt tgctctaaac
    11161 ttgagatgtt tatttctaag aaatattttt ccgtaaatgt taacccgaca gtgttatcga
    11221 gaataataac accaattgct aaaaggctta gtactaagaa aagtgtcagt cgataacgat
    11281 taaatggtaa tgagtttcga tataaaataa caaatgacaa gaatgtaatt gtaagaatcg
    11341 acattgtaat catttccatt gatgtatcac ttatttctgc accaaacatt tcgccaaagt
    11401 ttttacttat atagagtaaa aatgctccag cgaccatcaa caatcctgct ggcacgctgc
    11461 gactaatgac attatccata aatgtcccgc gcactctttc tttattcggt tgcatcgata
    11521 agaagaaggc gggaattcct aatgataata attcccacaa atacaagtgt tttggttcaa
    11581 agggatatct aatgcctgat ccttttggtc ctaaaaggcc cgcagtaaca aacataaacg
    11641 agaagataat cgcgaagatt gttttaacaa ggaataatga ccatgtcttt tgtaagttat
    11701 taattgctct tcgtccttca tcaacaattg aaggcaaaac atcaaagtta ttatcaagga
    11761 gcactaagtg actgactccg cgtgctgctg gactaccagc gcccattgca attgaacact
    11821 gcgcagcttt taatgctaag acatcattta cgccgtcccc aaccatcgct acaactttac
    11881 cgtcactttg taatttagtt acaatacctt ctttttgttc aggacgcact ctgccgtaaa
    11941 ttgtaaagtc ttcaagtgag tggtgacttg gatcatcaat tttagaaaag tctaataaat
    12001 tttcggcatt attaatcccg acttgacgcg ctatttcact cacgctaaca ggatcgtctc
    12061 cggaaataat ttttacttca acaccagcat cttggaatag tttaatgact tcactagctt
    12121 cagcgcgaat attatcttga ataacaataa gtgctaaagg ggtcatatca attggtagtt
    12181 ttttagcgat aaacggcttg cgagacgaag caagtactaa tacacgtaag ccgcgctcta
    12241 agtaaatgcg ttcaacttct tcaacttgtt tttgtgcttt tggaccaagc accatacttg
    12301 aagcaccaat gatataagtt ttaccttcca taattgcacc gctacatttt agttcgctat
    12361 caaacggtaa cactttggtg tattccatat attttctcgt tttaaaatgg tttttgagtg
    12421 ctatagcagt ggcgttttca tctttagtgg ctcttaaaat tgaggcaaca ataatgttta
    12481 aatcaatgtc ttttgcgcct ttaagtggca caacttcatt tactttcatt gtgccgtcag
    12541 taatcgtgcc tgttttatct aaacagagta cattaactct tgctaacatt tcaatcgaat
    12601 acatttcgtt cacaagtgtg ttttttgtcc cgagtctaat aactcctacg gcaagcgtca
    12661 tacttgttaa taaatacatc ccagaaggaa tcattgccac aagtgaaccg gcaatagagc
    12721 gcacactatc ttcaaatgtt gagccttgat aaaaggcatt aaacactaat agtaatgcca
    12781 tagcaatcac aataaaccca attactttaa atagtgaatt aattgaattt aaaagttcag
    12841 atgttggacg cttaaatact ttcgcccgta attgtaattt ttgtgcataa cctaattttc
    12901 caattgtatc aacttgatag taacatgtgc ctgttgtgac aaaagacccc gacaaaacgc
    12961 ggtcatcttt tgctttatga ataatgtttg gttcaccagt taatagtgat tcattaacac
    13021 caacactacc gtgcactacc acaccatctg ctgcgatttg atcacctaat tttaatttaa
    13081 cgatatcatc aagtacaaca tcctcacttg gaattaataa ctcaatccca tcgcgcacga
    13141 ccgttacttt ttgttcattt aatattaaga gtttctttag tgtgcgtcgc gccctaaagt
    13201 cttggataag tccgattaaa atgttggaaa aaagaatgac gagaaataac aaactttgcc
    13261 actctttaat ataaataagc gctgctccta ttgcgtagag caaaacatta aagaacgaaa
    13321 agaaattacg cattaatatt gaaaagattg acttaacgcc tttatttggt ttttcgttag
    13381 tcattttcgc tactttgcgg gcgttaactt gttgggtact taagccttca tcgtgttttg
    13441 gtgtataacg aattacttca tcaactttaa tttcgctttt tttaatgatt gttgttgccg
    13501 tacccggtct ttttttcttt gtttctacaa attcctccat aatgagtcct ttctattcat
    13561 tactcggtgc gcagcgctac gactggatct ttgtttgccg ccatcttact tggaattaat
    13621 ccagcggtga cggttaataa tatattaaat acaataagcg ctaaagctgc aagtggtact
    13681 aaaaacgcta tttgtccaat gttttgttca ggaaatagat tgttaagaat aacatttatc
    13741 ggtattgata atatatatgt tgtcccaacg ccaattaccc ccgcaagtag tccaataata
    13801 aacgtttccg cgttaaatag cctcgatata tctttcttcc tggccccaat cgcacgcagt
    13861 atcccaattt cttttgtcct ttcaatcaca ctaacataag taataatgcc aattaagaat
    13921 cctgacacaa caagtgagat acttgtaaat acaattaata caattgaaac aacatcaatc
    13981 attgtcgcaa ttgaatcagt aacaaactct actgcatcaa agattacaat tttatctttc
    14041 tcgtcttcgc gcgtattatt atattcatta atccgtgctt taatttcttt ttttgaatca
    14101 ccgtctttag caaaaattgc tactgattct actggtgaat aagctgtatt taataattct
    14161 tttaatagtg tttcgtaaat cgcgttgtcc ttacttatat ccgctaagtc cactccataa
    14221 attttgaggt tgcttaaaaa tttatcaatc cttattgttg ttggattaac tggttttaca
    14281 cccgaccaat cggtagtagc gctataaaat tcaattgcag tattaatatt ttctgcagtg
    14341 atatttgctt ctctgattac tacattatta acttgtgcct ttgcaacttt gttatgttta
    14401 tttgtttcat gcatatattt atttaatgct tgtgtataca ccattcctgg cgcgagcatt
    14461 tcaaactgaa ttcccggttt tggccgcaaa atgccagtaa ttttcagcat ttcaccaacc
    14521 gttgggtcat catataaata ttgtgggttt tgaaaacgat acttttcaat cgtgcgtggt
    14581 tgccccataa agtcaagcac tacatagtca tcaatattac ccaatgcctt tttataaata
    14641 tcatcgttgt gcaccatttt aaattctcgc ccaataatgt catcaaaatt aagcttttgt
    14701 acatttgcgg aattatcaac aatgcccatc cgttctaaag cttggactga gatacgatta
    14761 tatttatcaa gtacaaacat aatttcatta cgctctgtcg caaactttga gtctttacca
    14821 attacatcat aaaattcagt aatataggct tcgtcccctg gtaattgggt aaatacccca
    14881 aagggtagtg gcgtcacact gacataataa ggccactgta cagtacgcgc gttatcagtg
    14941 ggcggctttg ctaaaatttg catttgtgtt ggataattaa ccattgtact agcggcaagc
    15001 ccgtcttcct ttaatccgtc cactaaatct aagaattcag atgttatcct attatttgta
    15061 atggtaatcc gtgttgtttc tgtatcattt tcattataaa caacgatttc ttcaccgtct
    15121 ggatactcag tgtttttatc aggttttttt atgcctgctt gcatatccat tgtttgcgca
    15181 aaaacggcaa ttgggtaatt tgccattgtt tcccgctcca aacgattaat ataattttga
    15241 aatccgttac taaccgctgc tactaaccca ataccaataa tccctaatga tcctgcaagc
    15301 gcagtaaaca ctgttcttcc tttttttgtt aagatgtttt tataactgtt tttaagcgcg
    15361 gttaaaaagg acattgatgt tcccttgttt ttttctgttt ccgttggttc ttttactttg
    15421 cgcttctttt tactttgtgc ttcatcagtg tcactagtaa ttctgccatc taacatccta
    15481 attactcgat cgctatatcg tttagcgatg tcttcattgt gcgtaaccat tacaacaagt
    15541 cgtgttttac taatttcttt taaaattttc atgacttgtt cgcttgtttc actatctaac
    15601 gctccggtgg gttcatccgc taaaatgatg gaaggatcat taacaagtgc acgtgcaatc
    15661 gctactcttt gaagttgacc accagaaagt tgatttggtc ttttatataa ttcactttca
    15721 agatcaacac taaggagggc ttgtttagcc ttttcgcgac gttcactact ccccatccca
    15781 gaaagcgtta aagaaatctc aacattttca atcacactta agtgcgggat aagattataa
    15841 ctttgaaaca caaatccaac acgatgatta cgataagcgt cccaatctct atctttaaat
    15901 tcttttgttg atttaccttc aatgattaaa tcgcctgatg tatatttatc aagaccgcca
    15961 atcaagttta aaagtgttgt tttaccacaa cccgatggac ctaaaattgt tacgaattgt
    16021 tgtttaggaa aagtaaggtt tagccccttt aaaacatgaa gtggcttttt agccacataa
    16081 taatctttat aaatatcttt gagaacaagc ataaatatcc cctttcggat ttcactttat
    16141 acattgtcta actttttatt tatcttttca agataaaggc attattattt acattaattt
    16201 tataattgcg gttttacaag agacgggaat ttagttaatc tttgttacta tactaaattt
    16261 gaatcgtaaa tatatctttt tccacgataa gttcttcttc atattctaat tgagcgttaa
    16321 cttcaattgc atctattggt ttcagtattt gtgttatttg tcttagtctt aattcatcag
    16381 gaactctacc taagtcagta tatgaaattt gatatcgtga aattacaaaa ttttcatcga
    16441 aatcaattcg tataatttta tatgtttcta atttactgaa aaatgtagaa acaatcaacg
    16501 aattgttttt aaaattaaca tttgatttat taacaacatg tttttctaaa tcagtcagct
    16561 tagtctcaag agtacttata atataatcaa ttttatctat ttcagtattt atttgtaatg
    16621 tctctatttg atttgtgcca tatgtttttt cagtccatgt ttctttaggt acataaccgt
    16681 tgttatattt tattgtttta tgcaaatatt ctccgtccca taatatgttt tcaaaatttt
    16741 cgttaggaat ttctgttcct actccgccca ctaaagaatg atttaactgc atcatatttg
    16801 cttcaaaatt ccataatgta ccgtatggtt ttgattgcag ttgagcctta atattatatg
    16861 tttctatatg aaaataatct gtgttttcaa cctttgattt tttaaatgat gttatactat
    16921 aaaatcctgt ttgaaagtta tatccgtctg gatacacttc tttatattta atcaggaatt
    16981 tttctaattc gcttttttct ttttctttat ttgcacaacc gttaagaaca acactaaaaa
    17041 gtaatgaaat tataacaaga attctttttc gcataagaat tttcttacct cctcatcctt
    17101 gggaatcctg acggcattgg cccacctttt ttatatccct gcatttgttt catttgttca
    17161 agcgtatcta tcatttaaat caaaatctcc ttccgtaatc ttgcgcgttg ttttttgtct
    17221 tttgttcaaa ttgttgttgt gctttttcaa ccaatgttaa tacaacctct tttcctgcgt
    17281 tgtgtcaaat aatataaaac atgacaataa attgcacatt aatcttagtt tttaaatatt
    17341 ctaaattagt tttactaaat cttatcaaca taaattctgg tggttccaac gctaattctt
    17401 cctttatttc tactgccgac tgcttcagaa gtcatataat atctaaaaca tttagtaccg
    17461 ttagtgggga agttgataat atatgtcttc agttttaatg tttccaaatg ataaacatgt
    17521 cgttaatact agtcccaaag ttgtaagcat atgattaatc tcctttgcta aaactaaatt
    17581 tgaatcgtaa atatatcttt ttccacgata agttcttctt catattctaa ttgagcgtta
    17641 acttcaattg catctatcgg tttcagcgtt tgtgttattt gtcttggtct aaattcatca
    17701 ggaactctac ctaagtcaat atatgaaaat tgatatcgtg aaattacaaa attttcatcg
    17761 aaatcaattt gtataatttt atatatttca aattcactga aaaacgtaga aacaatcaac
    17821 gaattgttta taaaatcaac atttgattta tcaaaaacat atttttcata ataagacagc
    17881 ttagtcttaa gaatacgtat aacatattct attgtatata atttagtatt tattagtaat
    17941 gtctctactt gatctgtgcc atatgttttt tcagtccatg tttctttatg tatataattg
    18001 ttgcatttta ttgttttatg caaatattct ccgtcccata atatgttttc aaaattttca
    18061 atagggcctt ctcttcctgc acccaacact aaagaacgat tcaactgcat catatttgct
    18121 tcaaaattcc ataatacacc gtacggtatt gattgcagtt gagccttaat gttatatgtt
    18181 tcaatttcaa aataatcaga gctatcaacc ttcgattttg taaatgatgt aatactataa
    18241 aatcctgttt gaaaattata tccgtctgga tacacttctt tatatttaat taggaatttt
    18301 tctagttcac tttgttcttt ttgtggtttt gtacagccgt taagaacaac actaattagt
    18361 aaggaaatta caataaggat tcttttttgc ataattaaac tatatttcaa aactactcct
    18421 gtgtcaaata atagaaagcg cacattatga ttattcaatt atcgtatgta aacatttatt
    18481 tatatacata ttatattttt cgtttgttac ggaaaaatga ttgaatcact tccttacact
    18541 ctttagcgag caccccgcct tttatttccg gtatatggtt aactattcgc tcatcaaaaa
    18601 gattaaacgc ttcacccatg gcgccgcctt ttggatcacc agtgccgtac actacccgtt
    18661 ttaagcgact ccacattatc gcgcccgcac acattgcgca cggttcaact gtgacataga
    18721 gttcacagtc gacaagtcgc caattgttga gttttttact cgctttacga atcgcaatca
    18781 tttccgcgtg accaagcggg tcgttattaa tttcgcgttt attaaaacct cgggcaataa
    18841 ctttatcgtt aagcacgata actgcaccaa caggtacttc atcaattgcc gctgctttgt
    18901 gcgcttcttt aagcgctagc ttcatatatt tttcgtcttt attcatagtt taaattaaaa
    18961 accaccgcac atgattaagt aatcttaagg ctgctacgtt cccgtcctga cctggttcga
    19021 agaataacca tcgcgatggc accttcatta tagcaactac cagtcaggat gtaaaacttt
    19081 tgtccccaca aacgcttgta aaacttcagg aactaaaaca gtgccgtctg cttgttggaa
    19141 ttgttcaaca attgctggta ttaatcttga tgttgctagt ccagaaccgt taagtgtatg
    19201 taaatatttc accgcatttg tttctttatc gcgataacgc gccataccgc gcctagcttg
    19261 ataatctcta gcgttactaa cactgctaac ttctttataa atacccattg acggaatata
    19321 tacttcaata tcataagttc ttgccattga atgcgaacag tcacctgccg caagtttact
    19381 aatttggaag tgtaagccta acttctcgac aagacctgct gcgtgagcga ccatttcttc
    19441 gaaagctttt gatgaatctt gctctttagt gtattggaat atttcaactt tattaaattg
    19501 ataaccacga atcatcccgc gttcttcagt gcgataacta cctgcttcgc tacgataaca
    19561 cggagtgtag gcaaagtatt tctttggtaa ttcatcttct tttaatattt cgtcacgata
    19621 aaaattaacg agtgctgttt ccgaagttgg tagtaagaat ttacgcggtt ctgttccttc
    19681 taaccaaaac acatcttctt taaatttagg aaattgtcca gcggtaaagc cactttcata
    19741 atttaataag tgcggaggta aaatcatgtc gtaccctgct tttaagtgtt cactaacaaa
    19801 aaagtttaaa agtccccatt caagtagtgc tcctgcttta gtgtaaatcc acgtgccgct
    19861 tcctgaaatt tttgcagctc ttttataatc gataagtcct aaactttcac aaagttcaac
    19921 atgatcttta atcggaaaag taaactttgg cattttacca aaagtataca caactttatt
    19981 attttctttt ccacccgcaa gtaagtcttc gtctggtaaa tttggtaatt ctgctagtaa
    20041 attaaaaact tcttcatcaa gtttctttaa ttctttttcc ttagcttcgt tttccgcttt
    20101 caggtctttt acctcagcaa aaatggcctg aatgtcgcct ccagcctttt taacaaccgg
    20161 tacacttgcg cttaatttgt tgagttttgc tttagcttct tcaacttcaa cgcgaagttt
    20221 ccgccgtttt tcatcacgac ttaaaattgg ttcaggatca aaatcccacc cttttttaaa
    20281 aagtaaactt ttaacttttt ctgcttcatt tctaattaaa ttaatatcta acataatacg
    20341 cactcctttc ttaaacaaaa taaaacgccc taattaggac gtctttaacg tggtgccacc
    20401 taagtttgat acaaagttgt attctcatta ttgtcttaac gcgacaaacg aagcgattaa
    20461 gctttgttct ccgaggtgga attcataacc tttagttctc gccttcccac tatcagcgag
    20521 tcactgttaa accgcgaatt attactagtc tcttcactga acacatttat tttatcaagt
    20581 tttactttat tagcgagtat atatcttata aaacaagtta taaatcgctt ctgtaaataa
    20641 agcggcgtta taattttgtg tatattcgta agctttttcc gtaattttat ctgtttctgc
    20701 ttctgttcta attaagtaag ccgctccgtt atagtcattt acaatgtaag cttttgtctt
    20761 atcaacaatt tctttaaaaa ttacattttt ccgtaaaata agtggtactt ttaagtacat
    20821 cggctcaaaa agcgtcatta cattcatctt gtcgtaacct aaatcaatga agtaacttgc
    20881 accaataaca cccatccgat aaacatcttc tggtacaagc ttagtcacaa ttaagttcgg
    20941 tgaagttaat ttatccatcg ctttaagccg caatctatca ataaatgatg tgccaccgct
    21001 gatgaaaaca tagaaattat agtttggtac agcagcggct aagtcattaa gttggtcaat
    21061 atgttcactt gctttaatgt tcatcactgt tatggcatat ttttgatcat cgtttatacg
    21121 gaaatagcga cgaaatacat ctgttttgct tgtttttaac tcatcaatcc gtcgaacatc
    21181 aaccgctcca taaattacaa agattggaat ttttacaccg agttttcgta aaataacacg
    21241 cgcttcttgt gctggaacaa caataccgtc catcatatta attaattcaa gacgcgataa
    21301 aggaatgtat aactctccat cttttcttgt ttcaatcaca cgagcgtaag tgtcgttatt
    21361 agcgaagaac atccataaaa gcgtgggtat acttgcggca attgattgcc taatcacttt
    21421 cgaagattga ctattcaaat ttataaaatt agcgatatca atatcaggac taaatttagt
    21481 aacaagtgtt acatcatctc tttggcatgc tttttttatt tgatattcaa gcattgctcc
    21541 ttcaaagaag acatcaatct ttttatgtgg aaaagagatt aatattttca taactattct
    21601 tcgtgactag gagtcccttc accttcgtga tcatcttcgt caaaagcaat ccctttttcg
    21661 acaactgcga aagaacaaac aacatcatct gggcgtaaac gaatgacacg cacacccatt
    21721 gtatttcggc ttgttgtgct aatttcttta aagttggtgc gcacaaccat tccgctagcg
    21781 gtcgtaacaa ataagtcttc atctccctta attgctctaa ttcccgcaag tgttccagta
    21841 cgttcacttt ctttaatggt aattacacct tttgaacctc gttttgttaa acggtaatcg
    21901 ttagcaggtg ttaatttacc ataacctttt gttgtaagcg caaagattaa agcaccctct
    21961 aaacttgttg taacgccgac aacataagca ccttcgtcaa gtttaatacc acggactccg
    22021 gccgcagttc tgcccattgc ccgcacatcc gtttcataga aacttgccat tttaccatta
    22081 cttgaaccga taccaattat tgccgtacca tcagtaattt tgacatccat taattcatcg
    22141 ccttcgcgta aatcaatagc gattttacca ctgacattaa tgcgtgagaa ttccgctaaa
    22201 gttgtacgtt ttgtcacacc gttgcgggtt acatataaga ggtaatggtc ttcaccatat
    22261 gtatcagtcg acacaatagc acgtaccgct tcttcttttt caatatttaa aagattaatg
    22321 actggtaagc ctttcccttc acgacgatat tgcgggattt gataaccgcg tttacgatat
    22381 actttccctt tgcttgtaaa gaataagaca tcagtgtgtg tatttgtgtg taagataatg
    22441 tcaattattt cttcttcttt tgtgcgcatt ccttttacac caacgccacc gcgtttaatc
    22501 gttctaaatt cttcaggatc aacgcgttta atatagcctt ggtttgtgag catcacaacg
    22561 atatcttcaa ctggaattaa gtcttcgtta tcaatatcag caaggtcatc actaatttca
    22621 gtacggcgtt catcgccata cttagctttt atttcttcta actcttcaag gacaacatca
    22681 accatatggt cacgtgcact taaaattaag cgatagcgtt caatgttcgc aagtagttgt
    22741 tcgcgttctt tatttaattt gtcaatttct aaaccagtta agcgacggag tgtcattcct
    22801 aaaacttcgc gtgtttggat ttcgctaaag aggaagcgtt ccataagctt ttgactcgct
    22861 tcttcaccag attcactatt tttaataatt gatacaattt catcaatgtt atcagtggct
    22921 tttagtaatc cttccacaat gtggtcgcgc gcttctgctt gcgaaagttg atgttctgta
    22981 cgacgacgaa taatttccac ttggaagttt aaatattcaa caattaattc cttcatgttt
    23041 aaaatacgcg gagctttatt aactaaacat aaattcgtta ttttatatga agtttgtaaa
    23101 cgtgtttgtt ttaataaatt gtttaacacg acttctggta cgacatcacg acgtaattcg
    23161 ataaccacac gaattccttc tttgtttgat tcgtcacgaa tatcagtaat gccgtcaatt
    23221 tctttattac gcactaaaag ggcaattgct tcaattgttg ttgctttatt taatttataa
    23281 ggcatttcat caatgacgat ccatgtccga tcaccttttg tttcaaagtg cgtcttagcg
    23341 cgaacaatga tactgccttc accagtttca aaataatcta aaatcccttt gcgacctaaa
    23401 atgctcgcac cagttgggaa gtctggacct agcattccgt tttcaaaaat ttcgcgtggt
    23461 gtaacatcag gatttttcgc tactaggtta atcgcatcaa ttgtttcact taaattgtgc
    23521 ggcaccatat atgttgccat cccaacagca atcccgtcac taccattaac aattaagttt
    23581 gggaagcgtg atggtaatac ttctggttct ttttcactac catcatagtt accaccaaaa
    23641 tcaacagtgt ttaaattaat atcacgcacc atttccgttg caagtttttc catccgtgct
    23701 tcggtataac gcatcgctgc tgcttcgtca ccatcaatac taccgaagtt accgtgtccg
    23761 tccacaagcg gataacgtaa actaaatggt tgcgccatcc tgactaatgt tgaataaatt
    23821 gcttggtcac catgagggtg atatttaccc atgacatccc caacaatccg tgcgcttttc
    23881 ttaaacggtt tgtctggcgt cattcctgat tcatacatcc cgtaaactat ccgacgatgt
    23941 actggtttga acccgtctct aacatctgga atagcgcgac taacaattac cgacattgca
    24001 taatctaaaa atgatttacg aacttcatct acgacatcaa ccgggtgtat accggtgtca
    24061 atgccttcta attcatgctt ctcttcttct tcgagcatat ctttgatatt gtcttctacc
    24121 atgatgtcct ccttatacgt caaggttttt cacaaagagg gcgttttcat taataaactc
    24181 tttgcgtggt tcaacctttt ctcccattaa atcttcaaac acttgatcag cttcgattgc
    24241 gtcttcaatg tgtactctta aaagttttct gtttgcggga tccattgttg tttcccataa
    24301 ttgatgtgcg tccatttccc ctaacccttt atagcgttgg aaaggataac ctggacggag
    24361 atctaaatca actttaagct tttctaattg ttcgtcgtta taacaataat attgtttacc
    24421 gcgataatct actttataaa gcggtggttg ggcaatgtat acataaccat tttgaataag
    24481 tggacgcata aaacgattaa agaatgttaa tagtaatacg cgaatatgac taccatcaac
    24541 gtccgcgtct gtcataataa taattttatg ataccgtgct ttagttatgt cgaattcttc
    24601 ggcaattcct gcaccaattg cttgaatcat gttcccaatt tcagcgtttt catagattct
    24661 tgtttcttgc gctttttgca catttaagat ttttccgcgt aacggcaaga tcgcttgtgt
    24721 atgacggttg cgaccgtttt tagcactacc gcccgcacta ttaccttcaa cgataaatag
    24781 ttcgctttct tcaggattac gactactaca atctgctagt tttcctggca aggttgtaaa
    24841 ttcaatcccg tctttacgac gcacaccttc acgcgctaat gttgcagcgc ggcgcgcttt
    24901 agatgaaagt gaaatcttct ctaaaataag tttcgcttca tttggatttt caagcaagta
    24961 ctcttcgagt ttttccccta acacttgact gacaacagcc cgagtatcag tgttaccgag
    25021 ctttcctttt gtttgtcctt caaattgtgg gtttgggtgt ttaacggaaa taatcgccgt
    25081 cattccttca acgacgtctt cataagtaaa acggtcgtct tcatttttat gaatttttgc
    25141 ttctttcgca taattgttaa atacacggcc gaccgcaaga cggaaacctt gttcatgtgt
    25201 tccgccgtcc ccagtttgaa tgttattagc gaacgagaaa atcgtttctt tatattcagt
    25261 cgtgtactga atcgctactt ccactaagat gtggcttttt tgtccgttaa tttcgacatc
    25321 acctttgccc tcaacaaaaa taacatcttg agtaatcggt gttctatttt tattaatgtg
    25381 ttggacgtat tctgcaatac cgccttcgta ataaaattct tcgcgtttaa gctcttcgcc
    25441 acggcgatct tcaacattta ttctaatccc gcgatttaaa taagccattt gtcttagtcg
    25501 cgtgctgatt gttttgtaat caaattccac tgattcgaca aagataagtg gatcaggttt
    25561 aaatgttact cttgtaccgc gtttgtttgt gggaccaacc ttttttaatt cgccttcagt
    25621 gtgaccatcg ttattaaacg ctaagtagta ttcatctccg tctttataga cattaacttc
    25681 aactctttca ctaagggcat taacgactgc cgcaccaaca ccgtggagac cacctgctac
    25741 ttggtaagcg cctttcccga atttaccgcc agcgtgtaat acagtaaaga tagtttctac
    25801 agctgttttt ccagtttgtg agacaatacc aaccggcatt ccgcggccgt tatccgctac
    25861 tgttaccgaa ccgtctttgt taattgagac atcaattgtg gtacaaaagc ctgctagtgc
    25921 ttcgtcaaca gcgttatcaa cgatttccca cactaaatgg tggagtccag gaagtcctgt
    25981 tgtcccaatg tacatcccgg gtctttgtcg tacagcttgc aacccttcta atacttgaat
    26041 gtcttcagca gtgtaacttt cacgagcgcg catttcttgt ttagcttgct ctagttcttc
    26101 atctactatt ttatgttttt tctcttcact cattaggtaa tcctccttag ttctcggtca
    26161 cttgtaactt cataaattgc atgagcgtga ttgtcataat gtgttgcggt aataaatgct
    26221 tgagtgcatg tgagtaataa ctctattaat cgttcttgat gacgcttatc taattcactt
    26281 aagacatcat caagaataat aatcggtttt ttgtgtaagt ccttctctaa gaagtaaggc
    26341 gctagggcta aggcgataac aattaatctt ttttggcctt gtgatgcgtg ttcttttaca
    26401 ccgtgaccat taattgttgc tattaagtcg tcacgatgcg gtcctagtgt tgtactttcg
    26461 agaatgtttt cttttgctct aacttctgct agtcgtttta atccgtttct ctcaatgttt
    26521 tggacactac caagcgaaga ttcatatgtt agccttacgc ttaggtgttc atcacttaac
    26581 ttcaagagta cttcattaac tactttgttt aactcacgga tgaaccttgt gcgtttttct
    26641 acaatcgttt ttgctagttc gatgaatagt ttatcgatta agtcgagtgc ttcatcgtta
    26701 tcatccgttt ttaggagcac gtttctttct ttaagcaact tttcataacg cactaatgtc
    26761 gcaagataaa cttcatcagc gctacttaag tttgcgtcta agaactttcg tcttttagcg
    26821 ggacttgcgt cgaagaagag tacatcttct ggttcgaagg ttgtcgtccg aacatactta
    26881 cttaattgcg acaccttcgg gagcactttc ccatttatta gtatcctttt gctgcggttt
    26941 gttaactcaa tacttatatc tgtccgactt ggtatatgaa ttgttccgct tacaatcgct
    27001 tttgcggtat cgtgctttat tagtgctttt gtatcgtttg ttcgaaagct tttagcgaac
    27061 gcaaagagat gaatcgcttc tacaacattt gtcttaccga caccgttatc accaacgatg
    27121 acgtttagtc cactggtgaa gatgacatta agctttgggt agttgcgata gtttaatagt
    27181 tcaagatcgc taatcaacat ctttcactac taccttttgt gctgcgtaag ttattatctc
    27241 tccgccataa agcttacgac cccgtctagt ttcaaggatt ccgtcaactt ttacttcgcc
    27301 gttttgaatt acaaacttcg cttctccgcc tgttgaaacg agattatatt tctttagtgc
    27361 ttgctgtaaa gttatatatg gttttgtttc cacttatacg ctctccttgt cgctatgaac
    27421 gtattatacc acaaaaaaga aaagggaata taaatattcc cttaaattta ctactttttg
    27481 tgtccttaga acgacttttg cttgaatcgt gaggatttgc ccgtttttta ttaattttcg
    27541 ctctaaattg ctctatttta attatgtccg aactggtgta acaattaaca caagcgagtc
    27601 atctgtcgct actctgatgc taaatggttt gtgaccgccg ttaaacccga tggtgacatc
    27661 ttcagcaccg gtcgctttaa ttgcagagct tactaatgta tagttaaatg aaatttcaag
    27721 tggttcaccg acatacttac aaagcgctac ttcttcgcgt gcactttccg cgctgaatgt
    27781 tgaacgcgca gagacaacaa cactttctgg acttaatgac agtttaacga tattatcttt
    27841 ttccgctgcg taaagaattg aaacacggtc catcgctttt aagagttcat tagcgttagc
    27901 ttctaggaaa ctagcgtgtg tttttgggat aatgctacga gttggtggat attcaccagc
    27961 gacaagccgc gagacaacga gtgtatcatc aaacatgaaa ttaattttat tggcggacgc
    28021 actaacttcg attgtttcaa tgccaaccat tgtttcaaga agcttagcga cttcgttcat
    28081 aattcttgct ggaacattag cgacaaacga aacattgtct tttaatgtca caactttgtt
    28141 tgctagtctt gcactgtctg ttgcaactgc cgttaaagta ccattaactg catttaaatt
    28201 taatgcggtt aagattggcc tttgttctct cacactagcg gcaaatgaag tttgatcaac
    28261 gagtttaata aaatctttag cgccaatttc aaatacggtg ccatcttgac ttaaatcaat
    28321 atctggatag tctttagaat caatcgcgcc gagtcgatat tctgtgcgac cgtcactaat
    28381 ttgtgcgatg gtacggtcaa taatctcgac atcaatttcg ttcccttcca tttttcttat
    28441 tgattcatta agtgttttac tggaaataag tgtttcacct ggtaagaaca tccgaatgac
    28501 ttcgagatca ttaactcgca ttggaatatg cgtgcggatc gctagttcgt tgcttgaacc
    28561 aagtaatgtt aatccagttt catctaatga caataaaatg tttcctaaaa tgggaatagg
    28621 agactttgct tgagttggtc tagaggctat cgataaacct tttagaaatt gttctcgatt
    28681 gatcttaaac ttcataataa tatttaattc ctttcatatt taatgataat aataagggct
    28741 gtggagactg tggataactc cacaaaaaag cttattgact ctttttaatt ttagcacaga
    28801 agcaaatact ttattatata aatctattgt aaacgctctt ttatttcgtt tatagcttga
    28861 attaaagcag gatcttcttt caactgtttt tccacttttg taatagcgtt aatcacagta
    28921 ctatgatctt ttcccccaaa cgctttgccg atgcgcgcga acggaaggtc taaaatctcg
    28981 cgagttaagt acattccgat atggcgagca agcgcaactt ggcttgtgcg attacgacca
    29041 ataataagcg ctggagtaag gttatagtag tcagcgacaa cattaataat ttttgtttca
    29101 cttaatttcg tgacagcgtc tttaacattg attaaagact gcacactatt gagtgctact
    29161 tccatcgtaa cgtgttttgt tggggcgatg cttactacat aaaatagtaa acgattgagc
    29221 gcaccttcga gttcacgcac attatttgta aagttttcgg caaagaaagt aataacttct
    29281 tcatcaaaat cttcaatatc taaattattt aaggatattt tcattcgtaa aatatctttt
    29341 cttgtttcta agtcaggagc ggagatacta atttgtaaac cagagttaaa gcgtgtcact
    29401 aaacgctctt caataccttt aagttcgctt gggtgacggt cgctagttaa gacaatttgt
    29461 ttaccagagc cgaccaataa gttaaaaaga tggaaaaatg tttcacttgt cgacttttta
    29521 tccgctaaaa attggacatc atccattaat aaaacatcaa cagtacggaa aaagtctttg
    29581 agcgaaccgc ccggattatc acgaacaaaa cgaataaatt catcagtaaa ctcatcagcg
    29641 gtaatgtata aaaccttaag atttggggaa ttttgcttaa catgattacc aatcgcatgt
    29701 aaaaggtgcg tttttcctaa acccgaggca ctataaagga aaagcgggtt atataattta
    29761 cctggtgaat tagcgacaac aaccgctgct tgataagctt cgaggttgct tgagcttccg
    29821 acaacaaaat tatcaaaagt aagattagaa ttaactttcg cgtttttaaa aaatgtctct
    29881 ttcttttcgc taataatttc actaacacgc gggatttcgc ttttttcaat aaagcttatt
    29941 tgataattag attgcgtaat ttcaccaaca gtttcttcta taacatcgcg ataattcgtg
    30001 tttaacattg acttagctaa gctagaatta gcgataactg taatgattcc gttgttaatt
    30061 gaatgaatat aagtgtcagg gaagaaggct ccataaactc ttcgatcttg aattttttct
    30121 tggagcttga taagcgtacg atcccaaatt tgggtgattt ctgaaattga catgttcata
    30181 aactatttgc ctccgcaatc tttagcaata atttaacata attgggataa aaataaagag
    30241 ataagggaat aagatttttt tgtccacaat ttgttactta taaaagcgcg attatatgta
    30301 gttatccacc aaatccacaa aataatggtg tggataacat tgtggaaaac cttttccatg
    30361 ttgataatgt ggacttgcgc aagagttata cacagtgttt taaaattgtc cacaatgttt
    30421 ttgtttcata taatcggttt ttttgattaa ttaaacataa tcgtatgtgt tttccacata
    30481 aaagtggata tttttcaatt aattaataaa aaatgatgaa aaaaagccaa aaagaccgac
    30541 agatttaacg atatcctgtt attattcgtt gttgaaagaa gcatctacta attcgtgttt
    30601 aatagtcatt ggttgacttt tactatgtaa accactaaaa ttaatcgcga acaactttat
    30661 gttggattga atgaggagaa atattatgaa acgtacgtat caaccaagta aattaagacg
    30721 ggctcgtctt cacgggttcc gcgctagaat ggcgactcca ggtggtcgca gagttcttcg
    30781 taaccgacgt gctaaaggtc gtgaatcttt aacggtctaa gctgactact tttgaggaga
    30841 gttgtgaaac gggagcatcg gattttaaaa aacgagcaat tccaaaacat tataaaaaat
    30901 ggaaagctca caaaaacccc gctttgctat ggttactttg cttctaacga actcgaaact
    30961 ttacgtgtag gtgtcgctgt tagcaagaaa attggtaacg ccgtcacccg caataaaata
    31021 aaaagacaaa taagagcagt tgttagaccg tatgttaaaa cgcatactgg tgactttatc
    31081 attgtggcga aaaaggaaat taatgattca tccttcggtg atttagtaga tgcaattgcg
    31141 aaaatattcg acagtaacgg agattaacat gaaaaagaat aaaaaacgga ttataaaact
    31201 aggcgcaatt gtcgccggac ttttcgtgct ttcttcatgt accgcgagct tctgttccgc
    31261 gaaagacgtg ggctatatgc tttatgctca agattcaggg ttagttacag aagtcgttga
    31321 aggtactgaa acaaaagtac acaacaaagt tttacataag attattttag atgctcaaag
    31381 tcaaggctac gctactccta gcctagagta ttatgaaaag ttagatcaaa aagttttaga
    31441 ttatgcaatt ctaaattttg ataatagtga aaacttagaa ggcgccgcac ttaacgctgc
    31501 ggccttaaaa caaaatggtt acttaaagtt tttaggtacc aaaggcgcaa cagtagtgac
    31561 tgctgatggt aatgaactgt gggttaactg gacaatttgg aacaaagaaa ttggtgcaga
    31621 aattggttat gaaaatgttc ccgaccgcga ctttgctaat ttttataaaa cacaaattta
    31681 taacaaaatt aaggcaaatc gtgcttgtat tgcgctttat gacggcgaat atggaccaga
    31741 agatagtaaa attccagtcg aagccaagac atggaaaggt gcatggaaga aagggttcat
    31801 tgaaggctta attgtttatc ctgttgcagc actacttgaa tttttaacaa aatcatttgg
    31861 cgctggtgga tggggacaaa tttgggccat cttagttaca acaattattg tgcggggact
    31921 acttgtcctt atgacactta aacaaacggt gggtgcacaa aagatgcaag ccttacaacc
    31981 agagcttgca aagattcaac aaaagtatcc aaactctgac actaatcaat atgaaagaca
    32041 agctttagcg caagcacaaa tggcgttata taagaaacac ggaatcaacc cacttggatc
    32101 actacttgta atgttcattc agttcccaat ctttattggg gtatggggcg caatgactgg
    32161 ttctgctagt ttagcaagtg atagtgtttt agggcttaac ttaagtgctc aacttggtca
    32221 atcaatgatt aatggttggt ttaagggtgg ctggtggaca gcgtgggtgt tatttatatt
    32281 aatgaccgct gcacaatttg ttagcacaaa attatcaaca tggcttaaca agagcaaaac
    32341 aaaagaaatt ggtaaaacaa ccgctaatcc ggctgcggac aaacaacaac ggcaatcaaa
    32401 aatgatgatg aatattatgt ttattatgat tgtctttatg tcgtttactc ttcctgcagc
    32461 gatgggtgtg tattggtttg taggggcaat tatctcaatt gttcaaactg tagttgttca
    32521 taatgtgatg aaaggaagga aacaataatg cggatatttg aagcaaaaaa ccttgatgaa
    32581 gtcttagcgc tcgcaagtga agatattggt attcaaacaa aagacttagt gtatgaagta
    32641 cttgaagaaa agaaaggact tttcaaaagt tcaatcaaaa tcagtgtata cgaaacaact
    32701 gattacattg aatacgctga aaattattta cggttagtcg tggagtcatt aggttttact
    32761 cctgaattca ccacgagttt agatggcgaa gtcattaaaa ttgaaatgag cacaaaccac
    32821 aattcaattc ttattggtaa gggtggtaaa acattactcg cgttaaacga aattgtccaa
    32881 gtcgcgatga ccgttaaatt taaaaaacgc attcgagtat tattagatat taataattat
    32941 aaaaatgacc gttaccgtag agttactggc ttagcacgta aagttgcggg tgacgtcgca
    33001 cgttctaagc aagacgcaac acttgatcca atgccaagcg acgaaaggcg cgcgattcat
    33061 aatgcattaa gtgaatatcg caatgttacc actgaatcaa ttggtgcagg caaagaaaga
    33121 caagtcatca ttcgttataa aaagccagag aaaaaagaaa aggcaccaaa agaacctcaa
    33181 gacgaataaa acaagataaa caccaccgct aaggtggttt tttcttatta gataatagga
    33241 tatgataaaa tttaactatg ttagatacga ttgttgctct tgctactcca ccaatgatgg
    33301 gcgcccttgc catcatccgt ctctcaggac ctgaggcttt taatattgtt accaaaagtt
    33361 ttacgaaaga tttaactaaa ctagaaaaaa gcacgatggt gtacggcaaa ctttttgatg
    33421 acgaagaact tgttgacgaa gtaattgctt tttgttttaa agggccgaat tcttttactg
    33481 gtgaagacgt tgtcgagatt agttgtcacg gcagcatgct tatcgctaac gagataattg
    33541 aattaatgat taaaaacggc gcacgccttg cggggcgcgg agagtttact aatcgcgcat
    33601 atttaaacgg caagctcgat ttagtgcagg ccgaagctgt taacgatatg attaccgcga
    33661 cgacaaaaga agcaaagaaa ctttcactat tatcgctagt cggtgaaacg agtaaaatcg
    33721 tttatccgat aagagaaaga atcgcggacg tgatgagttt aatcgaagtt aacattgatt
    33781 atcctgagta tcgcgacatt gaagaagcaa gctacgcact tgttattagt gaagttgatt
    33841 atataaatgc ggagattaag aaattgattg atcaaggacg acaaggtcaa ataatcaaag
    33901 aaggaattaa agtcgctatt gttggtgctc ctaatgttgg gaagtcgtca ttattaaatg
    33961 cattacttaa agaggataaa gcaattgtta ctgatattgc tggtacaacg cgtgatgttg
    34021 tggaaggtga aattaattta cacggacttc gccttcttct tctcgatacc gcgggaatta
    34081 gagaaagcac agatgcaatt gaaagtatcg gtattgaaaa aacaaagaaa tcaattgcag
    34141 acgctgattt agtaatatat gtaaaagacg cagaaaatgt aagtaaaaaa gacgaattta
    34201 ctggtttaat taaaggtaag aaagtaatta ccgtttataa caagggagac ttagtaagta
    34261 agaaagatcc taatcgactt tacatttcag cgctcactaa tgatattggt gccttagaaa
    34321 aagcaattgc taaagttgta ggagttgatg aagatagtta tttacgacca agtttaaata
    34381 atgcgcgtca actagcgttg ttgcgcaaag cttatggcga acttgaaatc gctaaacaag
    34441 aggcgcttga tgaagtcgct attgaccttg ttagcgtgtc tttacaacaa gctttcttta
    34501 caatcaaaga aatattagga gaaaacgttg ataatgattt aagtaaagaa atcttttcgc
    34561 gtttctgtgt aggtaaataa agatgttttt tgatacacac tgtcatttaa acgataaaag
    34621 atacgaaaac gatattgaag ggtatgttga gcgtgctaaa gcagtgggcg ttaattatct
    34681 tttaattgtc gcatgggatg taaaatctag taaaaaagca attaaaatcg ctgaaaggtt
    34741 taataatgta tacgctgccg caggtattca cccagttgac gctgttaaaa caccgaaaag
    34801 tgatttaaaa gaacttgaaa aacttcttac ccatcccaaa gttatagcag taggggaaat
    34861 aggattagat taccactgga taaaagacga aaaagaaaga gcaattgaac acgaattctt
    34921 tatagcgcaa attgagcttg caaataaata taaaaaacca gtgcttattc atatgcgcga
    34981 cgctacaagt gatacatatg aagtgttaaa gagtaatatc ccaaggtatc aaggaataat
    35041 gcattcttac agtggttcaa aggaaatggc gttgcgtttt atagatttgg ggatgtatat
    35101 aagtctagcg ggaccagtaa cttttttaaa tgcgcgtgag ccaaaggaag tggcaaaggt
    35161 tgtaagcgaa gaaagacttc tacttgaaac agatgcgcct tacttaaccc cgcatccata
    35221 tcgcggcaaa ttaaatgaaa gtgcaaatat cccacttatt gctaagacca tagcaacgat
    35281 tcgtaatgtt tctgtcgaga aagttgcaga gtttacaaca aagaacgcta aaacattatt
    35341 taaattatga gtagcaaaac attgttaaat tgtatctttg ttgcggaagg aaagcgcgac
    35401 gaagaaagac taaaaaagtt agacatccct tacatagtaa ggacagacgg gctaaatgtt
    35461 ccacgtgaaa caacaaagca tttacaagaa ttagcgaaaa ttcatgaggt tataatctta
    35521 acagatccag acggaccagg gcaaaagatt gcagacaaac ttttacaaga aatacctaac
    35581 gctaaagtaa taaaagtaat aaagaaaaaa gcagtaaaga aaggaaaagt tggaatagaa
    35641 catattccgc taccgcttct agcggaatta attaaacctt ataagcaaga aacatttact
    35701 acttcttccg atgttacact tataaaatta ctagatttag ggttatctgg gccaaattca
    35761 caatttaaaa aaataaagtt agcgactaaa tatagtttat tagtagcgtc acttaaaaat
    35821 atgttaacgc aaatacaact attaaatata agttataaag aattaaagga agcactaagt
    35881 gaataaagaa gaaatcataa cattattagc aaaggaaaac ttgtcgccac aaaaaagaaa
    35941 gggacaaaac tttttagtgg atgaaaatgt agcgcgtaag attgtcagct ttcttgaacc
    36001 taattacatt gatgtaattg aagttggacc tggactaggt gcactaacaa aagagctgct
    36061 tttaaagacc gtaaatttta ctgcaattga aattgataaa ggttttgtgc gagtattaag
    36121 agcaaagtat ccacacctta acatcattga gaaagatttc ttagaatata gtgttccacg
    36181 tgaaacaaag gctgttatta gtaatattcc ttattacatc accacaaaaa taatagaaaa
    36241 ggtgttatta gaagcagaaa accttaaaaa gtttgtattt atgacacaaa ttgatgttaa
    36301 agaaaggttg ttagcaaaaa gcggaacaaa agcatatagt ccgctgcggg ttttacttag
    36361 tttgcttggt gatgttgaac aaaaactagt ggtaaccgct gataagtttt atccagtgcc
    36421 acatgttgat tcagcggtat tttgttttac acgatataaa accgatattc ctgtgcgtga
    36481 attttattcg tttttaaata acgcttttct aaatcggcgc aaaacactat acaacaattt
    36541 gaaaaacata tacgacaaag aagatatatt aaccgcttta aagaaacata gaattaacga
    36601 aaacgttcga gcagaagcac tatctcctaa cgatttatta acattatttg aaacattacg
    36661 ataattaaaa gggttaaaat gacaatcaac aaaaaactca aacatgaggg aaaattggaa
    36721 atttcagcaa tttatgatat attgaatatg taaatatcaa atctaaggaa tataacatga
    36781 aaaaatactg caaattttca actacattgt tgaatatttt agtaacggaa gttatttatt
    36841 atcgaacggt gtctatgtta taagtgacgc tgatttagat gcatttaata gtggtacact
    36901 tgatattcca aaaaataatg aagaaatata caattactgt tgttaataaa aagggaggaa
    36961 acgacaatga aaaataaaaa gaaattgttt tggttaatat taccatttac tttaattatg
    37021 ttcgctcttt tgtttgcgct ttatcctaca ccatggggag ccaaagaacc atacaaggtt
    37081 tttcattccc ctgaaaaaca tttaaaggaa gttgaaaaaa gagtgaatca aaaaatagaa
    37141 gagcgagaaa tggattatac aaatttacga gcacttcctc tttatgatga atataataaa
    37201 tttagctttg tacttgtaga atttgaaccc caagatttta tgattttaaa ggtttaccgc
    37261 actagtataa taagtgttaa aagaaccata tatttgcgtg acacaaataa tagttttggt
    37321 gtacacaccg aaaataataa tgaaaatagt cagtttaatc catatcaaaa ttgtgacact
    37381 tctaacaata aataatattt tttaaaagca aacaacggaa aaataggggg ttatataccc
    37441 gcgataaaaa cagaagataa atatattaac ctgatgtcaa atgaaaccat ggaactagac
    37501 tattataatt atgatactga ttatgcgaac ttgaacatct catatttagc taaaaggcca
    37561 ttatattttt aaattatttg gatctattaa atctaaggtg gaaaaaggtg gaaaaccaaa
    37621 ttaacaccct aaaactatta aatccaaggt ggacagacca ggaagtcaaa ttcctggtct
    37681 ttcattatta aagtgtctac gcttagggta tcaggacaaa tacttatcta cattgttaac
    37741 aaaagaaaaa agataatttt gagatgcacc ccaaatccta gacaatagga aaggagtgtt
    37801 ttcttatgag atacacacag ggatttatac tgttagttta ttataagcac tgaaaacgcc
    37861 ctaaaaacaa tgtaattttg atatcatata aatatgatag taactgcgaa tgcacgcttg
    37921 tccataagtg tagaaacaac gcttaatcca ttgactaacg ctttagagat taaaagcctt
    37981 gttttaccta ttgaatttag tgacacaatt acgattgatg tcttcaacac tgcctttatg
    38041 gatattgttc attacactaa taaggcgctt catgttaaag aaaaacggat tattgatgaa
    38101 gcgttactaa aaatgcggga acaatttggt tataagcaaa acttctctgt taaaatccat
    38161 aaaaatattc caacaggaca cggtttaggg agcggggcaa gtgatgcgac cgctgtcgtg
    38221 aaagctgttg cacaccttgc taaactaaaa ccaacaccag agcaatatta taatcttgca
    38281 gtcaaaattg gaaaagatgt cccttatttt ttagtaaata aacccgcagt gtacaattta
    38341 aacaaacaag aagtaacacc tataaaattt aaacataatc cacaggtgtt attaattatc
    38401 cacccagaca tttatgaaaa agaaaatgtt gttaaagact ttttggatta tggtgaatca
    38461 ctacaaaaac cgcttgaaaa agttgttgaa cttgcagaaa gtggttcttt gcgtgagtta
    38521 ggttctgtgc tttttaatga ctttagtgaa acgattatta aacatacacc agcattacgg
    38581 acagtgctta atgatctaaa acaattaaat gttgaagctt atggtatagc gggaacaggt
    38641 actacgattt ttgcactaag tgataatcga cacttattac gttttattag tgataaatat
    38701 cgcaaacaaa attatcaaac agtaatgaca aaagtcatca gataggagaa ttttatgaaa
    38761 agaaatgcca ttattctagc agcgggtaaa ggcacccgca tgaaatcatt aaatcaagaa
    38821 atatcgaaag taggttttga aatattaggc aagccactta ttaattgggt gttagatgcc
    38881 gcaaagccgc atgtaacagg aagaactgtt gtgattgtcg gttttggtgg tgaacacact
    38941 tctaaactcg tggaagggaa agctgaaatt gcttggcaac atgaacaaaa aggtacagga
    39001 cacgcaatca ttcaaacagc accgctactt gaaaaggaaa agggtgacac tttaatttta
    39061 agtggagacg taccacttat tactgaacac tccatcagcg gactacttaa tcttcacacc
    39121 aaagagaaaa acgatttaac aatattaacc gctaagccag ataatccttt tggttatggt
    39181 agagttttac gcgcaccaaa cggtgaagta actcgcattg ttgaacaaaa agacttaaca
    39241 ggtaatgaag gcgacattag tgaagttaat gctgggatgt atgtcgttaa taaccaaaag
    39301 ttattccaag aacttaaaaa cttaaaacca aacaataaac aaggcgaatt atatttaaca
    39361 gatattatcg gtttatttaa agaaagaggg tataaagttg gtgcctttgt gttaagtgat
    39421 cctgatgaaa tgcttggcac aaatgataga gcgcaacttg ccgaagctac ggaactcctt
    39481 aaaaaacgca ttaataaagc acatatgcta aacggcgtta cacttgaaga tccaaataac
    39541 acatatatag gaccagacgt cacaattggc caagatacaa ttattagtcc taacactacg
    39601 atattaggca atagcacgat tggtgttgca aatgaaattg gaccaaacac atatttaaag
    39661 aatatgaaga taggcgatta taattctatt atttatagtt acttagtaga ctcagaaatt
    39721 tgtaacaatg tcacgattgg tccgtttgcg agaatgcgac aaaaagcttt ggtaaaagac
    39781 ggctcacata ttgggaactt tgtcgaactt aaaaatacaa cattccacga aggcgttaaa
    39841 gctggccact tagcgtatat gggagatgct gaattaggcg cacacactaa tgttggtgca
    39901 ggagcaatta ctgcaaatta tgatggcgta aataaatcaa aaacaaaagt gggtgaaaat
    39961 gtctttattg gcacaaacac gactctagta gcaccccttc aaattgggga aggctcttat
    40021 cttgccgctg catcgatcat taataaagat gtaccaaaag acacacttgc tatcgcgcgt
    40081 tcacgccaag aaaataaaga aggctatgcg aaaataattc gtgaaaaaca aaaaaagaaa
    40141 tagaaattgc tgagaaatta acgaaagacg cttgtattga gcgtcttttt aaataataaa
    40201 aaacctcgct ccgaagaacg aggttaaagc tatcttaata gtaattatta cttagctttt
    40261 ttatgtttga ttgcagcttg ggctgcggct aagcgagcaa ttggcacacg atatggtgaa
    40321 cagctgacat agtctaagcc aacgctatca aagaattcga cagagtttgg atcaccacca
    40381 tgctcaccac agattcctaa gtgaacatca gggttgccct tacgaccgcg ttcaacacct
    40441 aagcgcacta attggccaac gccttcagtg tctaaggttt ggaatgggtc ttgttcgaag
    40501 atccgtgctt cagtatagtc aggaataaac ttcgtagcat catcacggct aaagccaaat
    40561 gtcatttgcg ttaagtcgtt tgttccaaag ctgaagaatt cggcttcttt agcaatttgg
    40621 tcggcaagta aggttgctcg tgggatttca atcattgtac caacgaggta atgaagtttt
    40681 tgcccactct tcttaattaa tgcatcagcg gttttacgta catattcttc aacgtattta
    40741 tattctttaa cgtctagcac tagtggaatc atgatttctg ggtggatagc ggcaccagtg
    40801 tctttttgtg cacgtaaggc ggcactaata atagcagttg tttgcatcac agcgatttct
    40861 gggtaagaaa cagctaaacg cacaccacgg tgacccatca ttgggttaaa ttctgaaagc
    40921 gattcaatcc gttcgcgaag aacagtgaca tcaactttaa gttctttggc taaagcaacg
    40981 atttgtgctt cttcggttgg taagaattcg tgtaacggtg ggtcaagtaa acgaacagtg
    41041 actggtaatc ccttcatcac cttgaagatt tcatagaagt cattttcttg gtatggaagg
    41101 atttgcgcta aggctgcttc acgttcttct tttgtagcgg aaagaatcat tttacgcata
    41161 aagaatacac gatcaacatc aaagaacata tgttcggtac ggcataagcc gataccagtt
    41221 gcaccaaagt cgcgaccatg ggtggcgtca agtggtgtat cagcgttaac acggacttca
    41281 agacgacgat atttgtcggc taattccatg aaacgaccaa agtaaccagt aagttgtggt
    41341 tcgactagtg ggatttcacc ttcatagatg ttccctgtag taccatcaat actaattaca
    41401 tctttagtag tgtaaacttt gtcaccaact ttcattgtgt ggccgacttc atcaatgatg
    41461 acatcgtgcg caccaacgat acatgttgta cccattccgc gcgcaacaac agcagcgtga
    41521 ctagtcatac caccgcgtga ggtgagaata ccagcagcgg aaaccatacc agcaatgtct
    41581 tctggtgaag tttcactacg aactaagagg acagtttcgc ctttttcttt acgggcagca
    41641 gcatcagcgg catcaaagct taaacggccg gtggcagcgc ctggagaagc tggtaaacca
    41701 gtagcgataa ctttctttgt ttttaagact gcttgatcaa agcctttgtg aagtaaactg
    41761 tcaagtgctt ttggatcaac gcgtaagacg gcttcttctt cagtaattaa gccttcatca
    41821 attaagtcac acacgatttt aagtgcagca gcggcggttc ttttaccact gcgtgtttgt
    41881 aagaagtata atttaccatc ttcaacagta aattcgatat cttgcatatc gcgatagtgt
    41941 ttttctaata aatctaaagt ttctacaaat tgtttgtaga cggcaggcat gttttcttct
    42001 aaccaactaa ttggtagcgg tgtacgaata ccagcaacaa cgtcttcacc ttgtgcattt
    42061 ggtaagtatt ccgcgaaaat gtgattgtca ccagtggatg gtgaacgggt aaaggcaaca
    42121 ccggttccac ttgtatcacc cttgttacca taaaccattg attgcacgtt tacagcagta
    42181 ccccattcgt aaggaatgtg gttcattcta cgatagatat ttgctctttc gttatcccag
    42241 ctacggaaga ctgcggtaac agcttcaatt aattggacac gagcgtcact tgggaattct
    42301 tcaccaagaa cttttttata atgagcttta aattgtttaa ctaattctct taaatgttca
    42361 gcctttaatt cactatcaag cttaacacca gctttaactt tcatgtcttc aagcattttt
    42421 tccatttcgg cacgtggttg gcctttaaca acgttgaaat acattaaaat aaaacgacga
    42481 tagctgtcaa acgcaaatgc tgcgttacca gtttcatttg ctaaaacttc ggcaacttca
    42541 tcattgagtc ctaagtttaa aacagtgtcc atcatcccag gcattgatac acgagcacca
    42601 gaacgaacac ttacgagtag tggatttttc ttaccaccaa atgttttacc agtttctttt
    42661 tctaattctg taactgcatt gtaaatttga tcaatcacaa attgtggtaa ttttttacct
    42721 tcattgtaat aaaggttgca agcttcagtt gtgacggtga acccttgtgg aataggaacg
    42781 ccaatgcgtg tcatttcagc aaggttagca cctttaccgc ccaagtaatt ccggcctaat
    42841 ttttcgcctt ctttacactt gaacgcaaag acatatcttt tttctgccat atttttcctc
    42901 cttttaataa gcagtattta acataccatc gctaattata atatcataga tattaaggtt
    42961 tattacaata aatcactcaa aaac
//
LOCUS       DAOSLP010000005        40924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_1122_length_40924_cov_10.897893, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000005 DAOSLP010000000
VERSION     DAOSLP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 40924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40924
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1122_length_40924_cov_10.897893"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(444..662)
                     /locus_tag="PK460_01015"
     CDS             complement(444..662)
                     /locus_tag="PK460_01015"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HOE53718.1"
                     /translation="MKINNCPYCNKDLKTGKLVYRYAYRAPKIIFNDGSEKKIDKLKL
                     FKDAVINKVFYCESCNIMLADLDSDSQK"
     gene            complement(678..1964)
                     /locus_tag="PK460_01020"
     CDS             complement(678..1964)
                     /locus_tag="PK460_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOE53719.1"
                     /translation="MNLKPEKYKKRIIDQQIEKYLNIFGAVCVEGPKWCGKTWSSLTH
                     SKSVIYIGDPEGNFQNRTLAEIDPKGVLNGDSPRLIDEWQEVPYLWDAVRAEVDFRGE
                     KGQFILTGSSTPKRKGVLHSGIGRIHTLRMKTMSLYESLDSSGEVSLLSLFDNEIKTN
                     LTKQFSLKELIELVVRGGWPMSLEQDKDFYGEIPKSYVNTLLNTDIHEIDNISRNKDK
                     FEALLKSLARNESTLAGNRVLINDINKYEKESIDDKTLVEYLNVLERLFIVENQNAFD
                     PNYRSSKRVARTPKRHFIDPSLAVAALGMSKEMLLNDLEYFGFLFEALVIRDLKIYAE
                     SIGGKVYHYKHHDTNHELDAVVELNDGRWIAIEVKLGASQIDDAAENLLKINNYFVNN
                     ENTSKPSALVVICGLSKAYYKRKDGVIVVPITALKP"
     gene            complement(2260..2649)
                     /locus_tag="PK460_01025"
     CDS             complement(2260..2649)
                     /locus_tag="PK460_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53720.1"
                     /translation="MNKTNLKNKISKRIIISEIIYVVVTAIISLLMFLPIYKDRATLP
                     GYDEEGNQIVVNLFYEKTPYQRLKAINIEWLLYLGLSLFLICIVILIISYTTNHKLDK
                     YKKVIFIISFGLILVLLLIAAIQITMY"
     gene            complement(2845..2931)
                     /locus_tag="PK460_01030"
     tRNA            complement(2845..2931)
                     /locus_tag="PK460_01030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2895..2897),aa:Leu,seq:caa)
     gene            complement(3060..3136)
                     /locus_tag="PK460_01035"
     tRNA            complement(3060..3136)
                     /locus_tag="PK460_01035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3100..3102),aa:Pro,seq:cgg)
     gene            3250..4158
                     /locus_tag="PK460_01040"
     CDS             3250..4158
                     /locus_tag="PK460_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53721.1"
                     /translation="MNKESGYRKTIISEYLNNLLHALNAYEGYSDGFRKLNGDFINYL
                     QARWVDLNIEVGDAIRVKFQRDRDYLSHPFLGIVIRLLEGARIHVPQREETLALLKKH
                     TNYPLHNFITFAFNFNRNDKDAPFEILSDDFIKIIFYLTNKKGYPHLENFDSFLEYKL
                     RISGVSIINLVYARRSALVEKNIDKVTEHLSLAAIQSGQDHRKFLGQKYYELATLFKK
                     HNDFAGKLLQKAVDFKNYEALLPLVKYYEEASDDKDDYTAIAFYYLVIAHHLEIPGAG
                     EKLKTYYEKGGVRPPCNKMLSKFFED"
     gene            complement(4160..8584)
                     /locus_tag="PK460_01045"
     CDS             complement(4160..8584)
                     /locus_tag="PK460_01045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOE53722.1"
                     /translation="MGNVSARFFASINYENDKKIGLTFLEWKKVDEVWHYTLLKEKPW
                     NFEDYLDFSDALMSIPYAYKVSYAYTKLPRIEDALALFKPWHLHTYLSESNYVIMVVG
                     NDTLRFTFTDEVSALDAKAVLYEFSLLLKELSYNFALETVVISSAQEEDEEDEFSLGS
                     ERPFIEDDDDDEEYFDEEDDTGVADCDPLDPNENCERSLEYHDGLEDKLKENYEKMVK
                     ERQKRRHAKGAFNLTEIKDLEHKEVDEGVDFDGEIYSYDRRPTKHGVTYTLGIGKDAH
                     GISVRFYINKRGAKQDEVLKALKTGRFVRVQGYIAIDNFRGQTKIVRGRDIDEIAAPP
                     LPTDDYEGRKRIELHLHTKMSALDAVSTIDEYCELAKNMGHEAIALTDHGVVQAFPEA
                     QKAGKKYDIKIIYGSELYVIDETLKVVNNPAPIILNNARYVIFDLETTGLSARYNEII
                     EFGAVRVEHGLVTARLDILINPGIVVPEHITRITKITNAMLRGKPKLDDVLDEILEFL
                     GDDILVSHNAEFDLGFLNANLRKRGRNPLNNPTIDTLALSRYLFPESRGHRLGVLARN
                     LEVFYDEDGAHRADYDAEVLNSIWQAMLTKLTEDDQYLTHAALAELKLTQAALQYLWP
                     AHATVYVKNKDGLRDLYKLVSVSHTEFSTDIPKTPRSMLLSHRDNLLIGSGCFNGEVF
                     ETAARFDEDRLLKVMEFYDFIEIQPYSNYSYLINMGELDSEEHLKKVIGDIKSAAQKL
                     GKPLVATGDVHYCLPSQKVFRDVYIVAKGLKNVNHPLNPYDRDKRPHFENPDQHYRST
                     TEMFEEMRKFGVFTEEELADMIINNPHRINDLVENITPFTKELYKPSIENVDELLENL
                     VFETAYEIYGNPLPTLISDRIHRELTGIINNGYAVIYYLAHLIVKKANDEGFIVGSRG
                     SVGSSVVAYFAKVSEVNPLPPHYFCPHCKFSNFRNEQRVLSGYDLPLAKCPECGHDLV
                     GEGQNIPFETFLGFEADKIPDIDLNFPEDFQKEAHNFTKELVGENNAFRAGTIQTVAE
                     RTAFGHVLNYYEKQGIYRDELNNENVAYLAYHIAGVKRTTGQHPGGIVVIPSEYEVYD
                     FTPVQYPAGNTESDWLTTHFDYDSIHDNLLKLDLLGHKDPVTLKMLCDLTGIKFEDVP
                     TNDRATISLFSSHEALNLKSNPLGEVTGALGLPEFGTNFVRQLLTEAKPKTFNDLVIV
                     SGLSHGTGVWRFNADELIRSEQATLGDVIGCRDDIMTFLIRQNLHEKDAFNIMESVRR
                     GRGVEEGLETKMREAGVPHYYIESCKKIEYLFPRAHAAAYVIMAVKVAWFKLNYPLEY
                     YATFFTIRGDNFDLKTMISSEEVILKELQKFEEQRKTSELNPRDSNIVENLQLTIEML
                     NRGFKISNIDLYKSEATRFKVDHENNQIIPPFIVIRALGEGTAESVVEARKNGEFISI
                     EDLVERTRLNTSNIENLKELGALEGLPESNQISLFDFM"
     gene            complement(8585..9841)
                     /locus_tag="PK460_01050"
     CDS             complement(8585..9841)
                     /locus_tag="PK460_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOE53723.1"
                     /translation="MEFLSTILNIILFLVMLGVLITIHELGHFIAAKIFRVYVFEFAI
                     GFGPKIFRKKKGETYYSLRALPLGGYVAMLGEEDAVPDEFVNEEIPKERSLIGINRGK
                     RALIMSAGIIMNLVLGYAIFLFANGVLPQTGLSIRLEVKEDSPVYAMGIRSDDRIDFK
                     ETKIDGYTIQYFGEGKLDAAALEPEYYVLFQPKSLDDLTFGGESLVLLKIGATDLASE
                     NSFYTDLKDTDVLTFTATFLRGETYETAERIEKVLTLSTEPKNPDKLTDGYKFVSAGF
                     NLTKFKYRNNFSETFTAANKDFIRSVTAVARGLKSLFSDGLKNVSGPVGIFNISSQTL
                     KNHGLAPYLFLWGLISINLALFNLLPFPPLDGWHLLVVTVEAITRQEISPKFKQIASM
                     IGAILLIGLTLVILAKDIFGFVGVIL"
     gene            complement(9854..10906)
                     /locus_tag="PK460_01055"
     CDS             complement(9854..10906)
                     /locus_tag="PK460_01055"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOE53724.1"
                     /translation="METNEIKPKTKRTSTIKISLNEISEATKKSMKTRTITSIILAVI
                     AVPALLLGGWYFLALVFFVSIIAVLEIMRGTKISKRYWCVYILAIVSTVALIFWIFIK
                     NNIMANIKAGYSWWDISKWVVENGFESIQVSSFLLATILIGLFILIVFDPHLGFDIAA
                     YLFLLVLFAGFGFQAFLFLRLYPQYIADRPDITFNTNIITSAFLIIYVILGTIMNDIG
                     AYFIGVLFGKNKMVPRISPNKTWEGFAGGVFFSIVVSFLFALIVSWIGYPVLPFLSHK
                     EWYYLLLISILMPLAANIGDLFFSATKRHLAIKDYGSILVGHGGILDRVDSLLMVSIV
                     TAMLIILINNGWSILV"
     gene            complement(10909..11628)
                     /gene="uppS"
                     /locus_tag="PK460_01060"
     CDS             complement(10909..11628)
                     /gene="uppS"
                     /locus_tag="PK460_01060"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOE53725.1"
                     /translation="MSKTKITNYENYGIKHIAFILDGNGRWAKARKRPRTYGHEKGFS
                     SLFDVGIAVSKRNIPFMSVYAFSTENWSRPKKEVDFLFRTLEKRLHSVLKKVQKHNIK
                     IVTSGDLKRLPESTQQAINYAKEESKNNTGTTLNICINYGGKDEIVRAIKRLAADESV
                     NIQALTPDTFENYLDTSGMPPVDLLVRTSGEMRLSNYMLWQLAYAEFIFTPTYWPDYD
                     EECLVRDLEEYAKRTRRFGGL"
     gene            complement(11991..12536)
                     /gene="frr"
                     /locus_tag="PK460_01065"
     CDS             complement(11991..12536)
                     /gene="frr"
                     /locus_tag="PK460_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOE53726.1"
                     /translation="MDEYALLAEERMEKSILNLQSEFSTLRTGRASAALLDRIECDYY
                     GEKMPINQISSISVPEPRQLLIKPYDKGDMRSILAAINASTLGINPINDGDTIRLILP
                     PLTEERRRDLVKQAKKFTEEAKIAIRNIRRDANTSIKNDKDLSEDMSKRAEEAVQKVT
                     DEYVKKADDLLKEKEEEILTV"
     gene            complement(12542..13261)
                     /gene="pyrH"
                     /locus_tag="PK460_01070"
     CDS             complement(12542..13261)
                     /gene="pyrH"
                     /locus_tag="PK460_01070"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOE53727.1"
                     /translation="MVYKRVLLKLSGEALAGDKKSGIYDPKVLSAILNFVKSFRHHQK
                     WEIGIVVGAGNIWRGKNAELIGFDPAAADYMGMTATFLNSLALKDYFTQNDLPAVVLN
                     AFAVEGLAEKTSPELAKKHLAKGEVVIFGGGTGKPFYTTDTGAALRAIDMNADLIIFV
                     KDGVDAVYIKNPHQYKDAQKITQVTYQELIDLKVEAMDLSALEVLNKTNITSFVIGMD
                     EVLNFEPLEEHMEIGTIITKR"
     gene            complement(13312..14172)
                     /gene="tsf"
                     /locus_tag="PK460_01075"
     CDS             complement(13312..14172)
                     /gene="tsf"
                     /locus_tag="PK460_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013489060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOE53728.1"
                     /translation="MASLIDKIKELRELTGAGMMDCKKALVENGEDVDKAKDWLREQG
                     ITTAAKKSGRIAAEGLALAKIEDGYAAIVEVNCETDFVSRGDDFKALVNTVLDLLLKH
                     KPANKEAADAIINPLLVEATVKIGEKLSFRRFELVKVEDRGATYIHMGGKIATVVLLD
                     KKNDELAHGLTMHIAANSPSYITTDDITEEDYNHERGIQLEITKNDPRLADKPEKMLK
                     NIVEGKIRKYFSDLVLAEQTYLMDDTKKVGQALKDLDNKVLVMFRYQVGEGIEKRQED
                     FAAEVFGQIK"
     gene            complement(14176..15315)
                     /gene="rpsB"
                     /locus_tag="PK460_01080"
     CDS             complement(14176..15315)
                     /gene="rpsB"
                     /locus_tag="PK460_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOE53729.1"
                     /translation="MSEEEKVVLTEEEVTPAMENVLPDENLPVITMKKLLEAGAHFGH
                     QTKFWNPKMKPYIYGSRNNVHIIDLEKTIVKIEEAYAALREIVTRGGKVLFVGTRKQH
                     AEHVKSEAERSGSFYMNNRWLGGTLTNFRTISTRVKYLRDLEMQEAEGAFDRLPKKEA
                     ILIRKEMKRLNDNFEGIKEMRKVPEAVFVVSTIDEANAIREARNLGIPVFALVDTNCN
                     PDVVDYIIPSNDDATKTVQLMLTLFADAIVEAKGGIPVLAYTRDAEPETETLEEALKH
                     NEPRARRTDSEERRQVRKAQTQHSDKPTRVRKTEAKEEGEAAPEVAEKPAAAAKPKKE
                     KEVKEPKAEKAEEPKVKAEPKAKAPKAPKVEEVKTEEVKTEKEGE"
     gene            complement(15487..16401)
                     /locus_tag="PK460_01085"
     CDS             complement(15487..16401)
                     /locus_tag="PK460_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE53730.1"
                     /translation="MNRPLQEFQDYLRYTRRFSENTVIAYISDIETFYLFLANQNMRD
                     DEVDKPLIRLYLKDQIDKYVSRRSIQRRLASLRHYYSFLQDKRYVKDNPFLTVQAPKA
                     TKALPRVLYEEEIRLILLANEKRTDYLRDRDQLIIELMFATGLRASEVINLTLQSFNL
                     NERFLMVLGKGKKERMVPFTDRVQVLLRNYLNGLRKELLAKRKSSQPTNILFLNAQGN
                     KLTIQGLRYILLSIEKKTGEYVNLHPHLLRHSFATYLLEKGANLRVIQELLGHESINT
                     TQIYTHVSEEKITKEYYTAHPRAKKKID"
     gene            complement(16394..17686)
                     /gene="trmFO"
                     /locus_tag="PK460_01090"
     CDS             complement(16394..17686)
                     /gene="trmFO"
                     /locus_tag="PK460_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOE53731.1"
                     /translation="MEQVVIIGGGLAGVEAAFFLAEKGIKVKLYEMRPGVMTPAHHSA
                     KLGELVCSNSLKSKELTNACGLLKKEMEIYNSLMTSVAARVSVPGGNALCVDRELYSE
                     EVTRIITTHPLIKVVNEEVVELPSHVPVIIASGPLTSKGLENTISKLIDTAFLHFFDA
                     SAPIIEKDSINMDIAYYKSRYDQGDDAYINCPFTKEEYYRFYDELINAKLAPIHEMDT
                     KYFDACMPLEVIAKRGPKTLRFGPLKPRGLGKDEHHKPYAVLQLRQDNVIGSLYNLVG
                     FQTNLTYAEQRRVFRLIPGLENAEFVRYGLMHRNTFVNAPRVLNLDKSLKVNNDIYIA
                     GQLSGVEGYVESAASGLYVALNVYAKIKGVKLEYPCETMLGSLMHYLNNANPENFAPM
                     NANFGIVRGATKQNRMEIAEKSLKITQKYKEYIESLNE"
     gene            complement(17732..19687)
                     /gene="topA"
                     /locus_tag="PK460_01095"
     CDS             complement(17732..19687)
                     /gene="topA"
                     /locus_tag="PK460_01095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOE53732.1"
                     /translation="MKLVIVESPSKIKTIERYLSDEYKVLASYGHIVDLSTRGRKNLG
                     INLETFEGDYVLDKSRQKVADTLKKAKNESSEVYLATDPDREGEAIAVHIAKLLNLDL
                     NTTKRLEFHEITRDGITNALAAPRLLNEDLFNSQEARRMLDRIVGFELSSLLNQKINV
                     KSAGRVQSAVLKLIVDKENEITAFVPEEYWTLKIDTKYDDALFSVNFNKSYAGDTKIV
                     NEAMKNDIVSKLAPKIVVTDVKSEIKHTNPKPPFTTSTLQQEASSKFKYSASRTSRII
                     QKLYEGIQINGIETGLVTYIRTDSTRLAPSFVANASQRIVKEYGAEYLGRTQARGKTL
                     QNVQDAHEAIRPTNLNFTPDSIKDSLTREEYNLYSLIYTRALASLMVGSKEQVTRYTF
                     NSNGTIFNATSTILIFPGFRKIYGKDEETVDDSALPQMHVGSELPIIDVITEQKFTEP
                     PARYSEGRLIKTMEELGIGRPSTYAPTIQILQKRKYVSSLKGTLKPTDIGFRQIDALS
                     EFFPSIISSDYTASMENELDKIANGEGDKVEFLSDFYSDFSQKIDDARKGMKKLPPEK
                     VGRDCPRCGSNLVYREGPYGRFIGCETFPKCKYTEFIVEYTGENCPKCNEPLTYRYDS
                     RNRKYIRCSNYKSCDYSATIKSTRRKK"
     gene            complement(19796..20578)
                     /gene="dprA"
                     /locus_tag="PK460_01100"
     CDS             complement(19796..20578)
                     /gene="dprA"
                     /locus_tag="PK460_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOE53733.1"
                     /translation="MNKKARDVLLYFASKYEGNYHAIINALRSKEPVDDALFNKYKED
                     WEKVDTITIIDEDYPALFKTVNNPPIVLFLKGNKELLFNLEKAIAVIGAREYSEYGKN
                     MTYEIVEGLVKEKFTIVSGLARGIDGFSHDACLRAGGNTIAVLGSGINYPYPKSNKKL
                     YDEVSKHGLLITEYPNDTKPEPDYFKFRNRLVAALTKAVLIIEAKYRSGTIITVGYAL
                     EKGSDVFCVPERAGRNSGCNRLIKDGAYLVESASDIIKLWPN"
     gene            complement(20628..21254)
                     /locus_tag="PK460_01105"
     CDS             complement(20628..21254)
                     /locus_tag="PK460_01105"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOE53734.1"
                     /translation="MTYFEFDNDVRKHGHLYLVGVDEAGRGPLAGPVFAAAVILPPFF
                     NDERIKDSKQLTATELLELSDLIKEKALDYQIVHVDAPVIDQVNIYQATILAMQKAVT
                     GLSHEFHFVLTDAMPLSNLEVGYEAIIQGDATSLSVAAASILAKVARDAYMNKLAKLY
                     PQYDFENNKGYPTRKHLQALDTYGPIKDVHRYSYKPVQDALNKQIKLF"
     gene            21439..22275
                     /locus_tag="PK460_01110"
     CDS             21439..22275
                     /locus_tag="PK460_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53735.1"
                     /translation="MKKRKFLLIPLLLGLLLTGCFGGDGPEDIIDGDDKSSQKTTNDV
                     AEEDILENMDYEKREGWPTQKLATFLSSERVNETVPALTNIATTYYHENNDANNKYFA
                     IVVPGANREADYKAILEGAGYTYSKLSWTSIAVNTEDTINIAYQYMQDHPVLDYAMYI
                     VISALKADSFRFDKLESDIGWPANQLQTFLSKYGITERVPSLTGGNYFAELTERNEFI
                     VCAPGVNFVDSYFGSLENEGYTVNGSNAVSTSGKVRINVTANDYPFFFFDATKIVIKP
                     VV"
     gene            complement(22346..23173)
                     /gene="ylqF"
                     /locus_tag="PK460_01115"
     CDS             complement(22346..23173)
                     /gene="ylqF"
                     /locus_tag="PK460_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOE53736.1"
                     /translation="MSKQIGWFPGHMQKATRKLIETLKMVDIIVELGDARIPHSSRAT
                     YLTAIIERKPNLYVLTKADLADPKRVSKHTEKLNVLAGNLNERKFVRQILAKIKEIGQ
                     PIIERDVRRGLKPRPLAVMIVGIPNVGKSTLINKLAGRKAASVQNKPGHTRANQYIRV
                     SEEIALIDTPGILSPNYDEEDTSMKLALIGTIRQEILPTHTMAEYLVNYLKTYYFEEM
                     VAFYDIDIKEDSNYQTVISAIATRRGIIGEDFDVSDRTEILILNEFKNGLIGRISLE"
     gene            complement(23173..23904)
                     /gene="lepB"
                     /locus_tag="PK460_01120"
     CDS             complement(23173..23904)
                     /gene="lepB"
                     /locus_tag="PK460_01120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOE53737.1"
                     /translation="MVIEEVLINEEISEAEINRKNKPRLRKALSVTWTILLVTAIVVS
                     LFVTYVDLTYTPVYVDGPSMQPTLNDFHHRYYEFGLMDERPKARNNIKRGDIVVFRRN
                     GINIIKRVIALPLETIQIIDNPTGDEVFITLPEATEKFKLDEPYLSTSNRYTTATYLN
                     DSSPKYENGLKEPLTLEEKTYYLMGDNRANSSDSRAIGPVHFDNISGKLVVIQGYTEN
                     VEVSESGKVKFIGQHYYVLWKWRYY"
     gene            complement(24027..24374)
                     /gene="rplS"
                     /locus_tag="PK460_01125"
     CDS             complement(24027..24374)
                     /gene="rplS"
                     /locus_tag="PK460_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOE53738.1"
                     /translation="MNKNLVNEINASQLRTDLPEFSSGDTVKVAVKIREGDKERIQFF
                     EGVVIAMQGAGVNHTFTVRKMSSGIGVERVFFTHSPVIDSVEVLRRGKVRRNKIYYIR
                     KLSGRKARIKEKK"
     gene            complement(24556..25962)
                     /locus_tag="PK460_01130"
     CDS             complement(24556..25962)
                     /locus_tag="PK460_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOE53739.1"
                     /translation="MKNLLNKLFKFEERKANLTSEILGGLVTFLAMIYILPTNAGILS
                     DMGMDKSGVFVATAIVSAIVSLVMGLYGNFPIVLSAGMGVNAYLAYVVYGALGSWQAA
                     LLVMFFAGIIFAVISLSPLRKMMIDAIPNDIKYIISAGLGAFIAFVGFKSSGFIVDSP
                     ATLVSLGNLRDPMVLLSLFGVLLVVIFMFSSNKRLNQLAIPIAMIIVVVVSVAVWNIF
                     ALPEATDAGRPIAFHFTNIKDMFSNPTSWGGQGFEKVAFKIFDKETWGTALSNPSTYA
                     FIFSLVLVNLFDTTATILAVGRGAGIIDADGKMVGGHKVVAADSFGAVICAPLGTSTV
                     TSFSESGIAVEMGAKTGLAAVVAGFMFLLSAFIYPIFYIFTEPAVTSMALISVGGLIF
                     VGNLKDVNFDDRIIGFTAFFTIMLILLTYSISDGLGLGLIMYVIMMFVSKRGKEVKIP
                     MYVVAGVFVIYFILKALV"
     gene            26116..26391
                     /gene="rpsP"
                     /locus_tag="PK460_01135"
     CDS             26116..26391
                     /gene="rpsP"
                     /locus_tag="PK460_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012094939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOE53740.1"
                     /translation="MAVKIRLTRVGRTHDPHYRIVAADSRYARDGRIIEQIGHYDPKK
                     DLKEAVIDRELALKWLGNGAQPSATVKALFRAQGINEEFAKSKKSGK"
     gene            26394..26648
                     /locus_tag="PK460_01140"
     CDS             26394..26648
                     /locus_tag="PK460_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOE53741.1"
                     /translation="MRDYEALVRTIITPLVKNEQALLIRQTNEGHRIIINIFAESDDI
                     AVLIGRRGVVANALREVVSVAGKLDEEHVFLRFEALDENK"
     gene            26689..27204
                     /gene="rimM"
                     /locus_tag="PK460_01145"
     CDS             26689..27204
                     /gene="rimM"
                     /locus_tag="PK460_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOE53742.1"
                     /translation="MNKDYLKIGYIVETHALKGALKIKPTTSFAEERFEVGNKVLVKN
                     NTTSKTETLTIQSVREQKGLLIVTFAEIDNINIAENYLKGFLFIAKKDITLEEGYFFF
                     DDLLGMKVVLEDETLVGEVIEILEYAAYHTLRVKREDGSNILIPYIEEFIVSTDLASK
                     TIVFRPIEGML"
     gene            27201..27974
                     /gene="trmD"
                     /locus_tag="PK460_01150"
     CDS             27201..27974
                     /gene="trmD"
                     /locus_tag="PK460_01150"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOE53743.1"
                     /translation="MKITILTLFPEMFTNFLETSIIKKARLQGHVTFDIVNIRDFADN
                     KWNRVDTPPVGGGAGMILQAEPIVKALESVDSGHKLLLSPRGNVLTNMKATALSQKEH
                     LIFICGHYEGIDERVNNFIDEEISIGDYILTGGELGAMVISDAAIRLLDEVISTESLD
                     IESFDYHLLEYPQYTEPYDFRGYTVPTILYSGNHAAIRKWRLKESLRITRERRPDLFK
                     KITKDNEIKKLLKEIDDDVIGEWEKDAIEKGSRFTKNKT"
     gene            28275..28565
                     /locus_tag="PK460_01155"
     CDS             28275..28565
                     /locus_tag="PK460_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53744.1"
                     /translation="MHDRKTAFNFATWLFIYDFGKCDIRNPYPFLALLFKKMELDKDI
                     QDNLNNRFDTPYDRFLDLYVDVLISSKIINANEADYLIPDKDTMLLDELEKL"
     gene            29064..30368
                     /locus_tag="PK460_01160"
     CDS             29064..30368
                     /locus_tag="PK460_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE53745.1"
                     /translation="MIKRDFYINKLINSKGNGKVKVITGLRRCGKSYLLFKLYKEYLL
                     SQGISEDQIIAYSLEGYENIHLRNINVLYASIKARLKDDNKKYYLFIDEIQNVHPMQN
                     PYVESKDIITFVDLMLEFVKLDNLDIYITGSNSKMLSKDILTQFRGRTHQIRLLPLSF
                     NELYNHFGNTPDLLDNYFMYGGMPEIYTKFRNEEAKRDYLKYLFEETYIKDVLERNNI
                     QNDTFILGTLLDFISSSVGSLISPSKISNRFLSEQNIKISSATVDRYLEYFDDAFVLS
                     KANRYDISGARYYKSNNKYYYTDVGLRNARLNFPLKDQGHIMENIIYIDLIKRGYKVD
                     VGIVHDNYRVDGRLTQKQLEVDFMVTGANERYYIQSVLTLDDLTTTERETRPLNKIKD
                     NFIKIVVIKNYTGSFYDKDGIFYINLKDFLLNENLAEITKRW"
     gene            30593..30967
                     /locus_tag="PK460_01165"
     CDS             30593..30967
                     /locus_tag="PK460_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE53746.1"
                     /translation="MTISEKIKKLRKAQGLTQAELAKGVNVSRTLINKYENGAATPTD
                     GNFISPYAVVSKNGLKYTDLSRTITDAFANEEILDMQGITEAISRYYFTNNEKLDGIA
                     VAPEYQERFERLVSDAIEYHEE"
     gene            30979..31266
                     /locus_tag="PK460_01170"
     CDS             30979..31266
                     /locus_tag="PK460_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53747.1"
                     /translation="MNISNEEKLMYKVMKAIYDSGIPVSFKGSLVLKAFLLESGYTKD
                     TRHTVDIDANWNGKTTPTMEQITESLQKALDKAKINLDVTYFRTIGLLDLN"
     gene            31323..31679
                     /locus_tag="PK460_01175"
     CDS             31323..31679
                     /locus_tag="PK460_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53748.1"
                     /translation="MVKTKIYEVSGLCFKGVIPSQMIADKLSVISIDKVFRRIKDLVD
                     LYYLSHVCDFNSEDVLSMLKNNGKELGKFDGFLNRTKDLKHSYEKFRFAGGVSKPTFE
                     EVYLAVKTFIVDVLPQ"
     gene            complement(31964..33310)
                     /gene="ffh"
                     /locus_tag="PK460_01180"
     CDS             complement(31964..33310)
                     /gene="ffh"
                     /locus_tag="PK460_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOE53749.1"
                     /translation="MMAFESLTERFSKILKKVRGQSRLTEKNMDEMLQEVRIALFEAD
                     VNFKVVKEFVNNIKEKALGTKVYDTLNPGQMVVKIVNDELVELLGSTDTELIFEKNKP
                     TIIMLVGLQGTGKTTTAGKLALHFKKKMNKRVLLAAADVYRPAAIDQLDTLAKQVGVD
                     IVNLGDKVSPVEIAKAAKKKAYDDRYDVLIIDTAGRLTIDETLMNELKEMKAAVTPTE
                     ILLLVDAMSGQDAVNTALSFNEKLGLTGAIMSKLDSDARGGAALSIKHLTGVPIKFAG
                     VGETLEDLDIFYPDRMADRILGMGDVLTLVEKAQQQLDEKTVKKTTRKIMEGEFDLND
                     MLEQMKQVQKLGKLGGILKLIPGMPKITPEQQELAEREMKLFEVIINSMTPEERSNPA
                     ILRNSRKVRIAKGSGTSNQEINKVIKKYEQMKQMMKQMKGYKKGGPMPPGFPGMGR"
     gene            complement(33297..33623)
                     /locus_tag="PK460_01185"
     CDS             complement(33297..33623)
                     /locus_tag="PK460_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53750.1"
                     /translation="MDELKEYMYINELLLIYGKTLTKRQLKIMESYYKYNLGVQEIAE
                     ELNITKAAVSDAIKVSTKHLENLESIVGHYAYRQQVEALHEEIKNTSTLQEVKELLSK
                     EEDDGI"
     gene            complement(33629..33907)
                     /locus_tag="PK460_01190"
     CDS             complement(33629..33907)
                     /locus_tag="PK460_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53751.1"
                     /translation="MVVISLLSLVVSFLVFMGVRFAVYSLNPSNYLFADILIIIIGSF
                     IVSLIFAIRQFRYMGRDKWRSQFFHGFVLRTFLINFAIIFIISLLWSW"
     gene            complement(33907..34887)
                     /gene="ftsY"
                     /locus_tag="PK460_01195"
     CDS             complement(33907..34887)
                     /gene="ftsY"
                     /locus_tag="PK460_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOE53752.1"
                     /translation="MGLISFLKKKFGGKKNETTDKYVVGLEKSKANFSSKLKRLAKRY
                     EKVTEEYYEHLLEILIGADVGVTLTYEIIEATKEEALLNNLTDPEAINDLLVDKMFIG
                     YLKQGEDIVNEIQFADNGPTVLLVVGVNGSGKTTSIAKLAHRYKKEGKKVLLVAGDTF
                     RAGAVAQLEVWAKRLDVPIVTGKPNADPASVAYDGVAEGKKINADLIIIDTAGRLQTK
                     VNLMAELSKIRRVIGDQIENAPHEVFLIIDATTGQNGVLQAKAFAEATGLTGVVITKM
                     DGTSKGGIILAIRDEIGVPVRFIGLGEKMEDLDEFDLEKYLYGLCLGEDE"
     gene            complement(34889..35569)
                     /gene="rnc"
                     /locus_tag="PK460_01200"
     CDS             complement(34889..35569)
                     /gene="rnc"
                     /locus_tag="PK460_01200"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOE53753.1"
                     /translation="MSKLNSLFTKLGITPSNEGLFEEALTHSSYKTKDKDAHDYERLE
                     FLGDAILNMLIASAIYKSHPDKKQGELTNMRKNFVRTQTLATLGRKLELERYIRTGKS
                     ITPQDLYSNDKYFEDVFEALIGAMYLDQGLKVTEDFILRMLGSAIEQYDFNQIFDYIS
                     TLQEFMQADKRGLPQYITEPSGYDNKEEAFISTVTYDGIILGCGYGANSKEAEQNAAK
                     NALEKVAR"
     gene            complement(35553..36539)
                     /gene="plsX"
                     /locus_tag="PK460_01205"
     CDS             complement(35553..36539)
                     /gene="plsX"
                     /locus_tag="PK460_01205"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOE53754.1"
                     /translation="MKLVIEMMGGDYGFESTIPALIQFKKDYPEVELFAVGRKEDLAP
                     VADYATIVDARDVVKIDATPLEVLRAKESSLLKAINTVIEEKADALISAGGTGPYLSA
                     ATLKLKLIEGVERAAILAPFPTKIKGKQVAILDIGASNENTASHLYQFALMGQQYARA
                     VFGVEAPKTYLLSNGTEDTKGSPLVLEAFKLYKEKELPYFMGQMEARDALNGEADVVI
                     ADGFTGNVLLKSIEGTAKVLMDSVKGAFKRNLFSKLGYLFARKGFKNMAETFDYKNTG
                     GAILIGVNNVVVKAHGNSDPRGFYSAILVGYKMAKADIVNKVREGLKNVETK"
     gene            complement(36790..38754)
                     /locus_tag="PK460_01210"
     CDS             complement(36790..38754)
                     /locus_tag="PK460_01210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOE53755.1"
                     /translation="MAKISNSSRIINALAKYDIHTFRDVIFHLPRRYLDMSYTDEFKM
                     NTNEKVTLKLKVTSAPLLIKPQRVAIIRFTAVSESGATYKIVAYNQRYILNAVKIGDL
                     ITVYGTINLRLLELTVIKLFPRVLSEEEQLSPLYSLPSEVGNHEFIRIVQKALKIESV
                     TSRVPRPLADKNGLISLNDALKRVHNPRDLNDVFEGLKALKYEEGLEYFIKLLLIKRA
                     NSELTNSKKTIVNLKDANEFVKRLPFKLTKDQLTAVREIGYDMNKETLMYRLLQGDVG
                     SGKTVVSFVALYINYLRGAQGVLLAPTESLAKQHYETLLSFFKDFDLRIELLIGALTP
                     SVKTKLKEDIQHGLVDIVVGTHALFSEDVFYPKLELVVIDEQHKFGVNQRNTLLNKGE
                     KADLLLLSATPIPRTLANVIYGDMDISTIESFPHTGRQVNTIITTSASKNVIPLIDKT
                     LSENRQVYIVAPKISFGDKTNVLALYERFSARYPHLVGMLHGELASEEKDAALLNFKS
                     GVTPILVSTTVVEVGIDVKSAGLMIIYEPHSFGLSSLHQLRGRIGRDGKRATLCLVSE
                     EEDNYKLEEFAREDNGFKIAELDLALRGPGELTGEKQSGMPSFMYLNVIKDLKLITII
                     KEDALEVINNPTNPHYFPLLKVINDALEKD"
     gene            complement(38763..40439)
                     /locus_tag="PK460_01215"
     CDS             complement(38763..40439)
                     /locus_tag="PK460_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOE53756.1"
                     /translation="MKEINGITLKEMFISGANHLYNNYPAIDALNVFPVPDGDTGLNM
                     NLTVSSGAKEISNLNDTNVYRIARAFSKGLLMGARGNSGVITSQIFRGFANGLEGKET
                     MNAVQFGEAMLVGKDVAYKAVMRPVEGTMLTVIREAAEVLNEKAKPDWSIVKALEVMV
                     AEAKASLERTPDLLPVLKEVGVVDSGGAGVVAILEGMLAAITTGIIQKESATATEVVP
                     QYEMAGARITDEEEYGYCTEFILRIGAGEEVKKTFNEERFASVLNSQGNSIVMVRDED
                     ILKVHIHTLRPGTTLTYAQQFGEFVTLKIENMTEQHTHLSDHGKDYFNTNPQVTSASM
                     EMKDYALISVASGEGLSDLFKEYRVDYVVKGGQTMNPSTEDFLAAIKKVHAKTVFILP
                     NNGNIILAATQAAEVTNEEGVVNVHVIPSKTIPQGLVAAMNFNPEVDTEHNIEIMTEA
                     LDAVETGQITFAIKDTSIDGFEVKKDYYMGLQGKKILCSLPDKFDALRELLAGMIDEL
                     TAIVTIIYGEDVEESAAEELLAELEEKYSAVDFELVNGGQPVYSFIVGLE"
     gene            complement(40455..40805)
                     /locus_tag="PK460_01220"
     CDS             complement(40455..40805)
                     /locus_tag="PK460_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOE53757.1"
                     /translation="METKNKTPFGGITISEEAIASAAVDAATSCYGVIGIANQPPKSW
                     LREIFNVDPEPGVVVKKVSGSWHVSLYLNVMYGLKLTEIITSVQDQVKYSLEKTFDIK
                     FKAINIYIVGVEER"
BASE COUNT        12776 a         7896 c         6607 g        13645 t
ORIGIN      
        1 gatattaaca aatcaccctt ctttttttga agaattaggt ctcacatcat ttacatcttt
       61 acaaccagtg ggattattat aattaacgag tgaataccct ctatgtatcc ttaacgagta
      121 aaattgtgca actatgcatt tttcaaccca aatctaaaaa tcaaatgtcc caaacataaa
      181 aatatgacga tattaagtca attatcgtgt tttagccaag aaaaaaggtt tgaaattgta
      241 tcaaacctta cgtttcaata tgtgttgtcc gtacaaagtt ttacccgcta aaatcacata
      301 atgtacaaag ttttacccca atatggcaca aagttttacc ccttactata tttataccct
      361 atttttgtaa aagttcagac cctaatttgt agttttcaac caattaaaac taaatatcat
      421 ttaaatgttt ttattttaat aaactatttt tgtgaatcgc tatctaagtc ggccaacatg
      481 atattgcatg attcacaata aaaaacttta ttaattacgg catctttaaa cagtttaagt
      541 ttatcaatct ttttttctga gccatcatta aaaataattt taggtgctct atatgcataa
      601 cgataaacta atttacccgt ttttaagtct ttattacaat atggacaatt attaattttc
      661 attttttacc tcttctctta tggcttaagt gccgtaattg gtactactat tacaccatct
      721 tttcttttat aataagcctt tgacaaaccg catattacga ctaatgcact cggcttactt
      781 gtattttcat tattaacaaa gtagttgtta atttttaaaa gattttctgc ggcatcatca
      841 atttgacttg ctcctaattt aacttcaata gcaatccacc taccatcatt tagttcaaca
      901 acagcatcta actcgtgatt tgtatcatga tgtttataat gataaacttt cccaccaata
      961 ctttctgcat atatttttaa atctctaatt actaaagctt caaataaaaa accaaaatat
     1021 tctaaatcat tcaataacat ttctttagac atacctaaag cagcaacagc tagtgatgga
     1081 tcaataaaat gccttttagg agttcttgca actcttttac tagatctata attggggtca
     1141 aatgcatttt gattttcaac aataaacaac ctttctaata cattcaagta ttccactaaa
     1201 gttttatcgt caatactttc tttctcatat ttattaatat cattaatcaa aactcgatta
     1261 cctgcaagtg tactttcatt tcttgctagt gattttaata acgcttcaaa cttatcttta
     1321 tttctagaaa tattatcgat ttcatgtata tctgtattta ataatgtatt aacgtatgat
     1381 ttagggattt caccataaaa atctttatct tgttctaaag acatcggcca tccacctcta
     1441 acaactaatt caattagttc ttttaatgaa aattgtttcg ttaagttcgt tttaatttca
     1501 ttatcaaata aactaagtaa tgaaacttca cctgatgaat ctaaagactc ataaagagac
     1561 attgttttca ttcttaaagt atgaattctt ccaataccac tatgtaacac gccttttctc
     1621 ttaggtgttg atgaacccgt taagataaat tgaccttttt ctcctctaaa atcaacctcg
     1681 gctctaactg catcccacaa ataaggaact tcttgccatt catcaattaa tctaggtgaa
     1741 tctccattta atactccttt tggatcaatt tctgctaagg ttctattttg aaaattacct
     1801 tctggatcac caatataaat aacacttttt gaatgtgtta aagatgacca agtttttccg
     1861 caccattttg ggccctcaac acaaacagct ccaaaaatat ttagatactt ttcaatttgt
     1921 tgatcaatta ttcttttttt atatttttca ggttttaagt tcatattcac atcacctcta
     1981 atataaatat acatcaaaca tctatttttg tcaattttat tcctgttttt ttgttaattt
     2041 tattccgtgt ttttagcctt ggctgttccc gttttttagt gggttgctga aaatcattta
     2101 caatgtcctt ttataaagaa aaaagatctg attcataaaa tcaaatcttt cgatgttttt
     2161 tgtgttgtcc atacaaaggt ttaccgcaat atggcacaat gttttactcc tttgccttta
     2221 aaaagtgaat ataatggtct atatatttag aaaataatct caatacattg ttatttggat
     2281 ggcagcaatc agcaataaaa caagaataag accaaatgat attataaaaa tcactttttt
     2341 gtatttatct agtttatgat tagtggtata agaaatgatt aatataacta tgcaaataag
     2401 aaatagtgaa agtcctaaat acaataacca ctcgatattg attgctttga gtctttgata
     2461 aggagttttt tcataaaata aattaacaac aatttggtta ccttcttcat catatcctgg
     2521 caaagtagct cggtctttat atattggcaa gaacatgagc aaagaaatta ttgctgtaac
     2581 tacaacataa ataatttcgc taataattat ccttttgctt attttatttt ttaaatttgt
     2641 tttattcata tgcctccact tcttagtata atcttatcac acttgatttt gaaatgatac
     2701 atgaaacgat acaagaaatg atacaagaaa attctaatat gtacatttaa agaaatactt
     2761 aaaataaaaa aagtaaccat gaaattaatc aatagctact tatttctcag ttttaatagt
     2821 taaaaccggt gtttcagaat tttttggtgc ctccgatcgg acttgaaccg atacagtatc
     2881 tctactaatg gattttgagt ccatcgcgtc taccacttcc accacggagg cacaagataa
     2941 ttataaaaca ttaacaacat aaattttttt ataaagctaa attcattaaa accttcgctg
     3001 agaaattatg tgattagtca cacttttttt caatgaacat catttcttta aaataaaaat
     3061 ggtcggaatg acaggattcg aacctgcgac cttctggtcc cgaaccagac gcactaccaa
     3121 gctgtgctac atcccgactg cacacatatt atacgctgtt tttaaaaatt accaaacttt
     3181 tgtgtcataa acactgttga ttttatctat acacgcaatg ataaccttaa aatttgataa
     3241 aatatttata tgaataaaga atcagggtat cggaaaacta taataagcga gtatttaaat
     3301 aatcttttac atgcacttaa tgcctatgaa ggatatagtg atggtttccg taaactaaac
     3361 ggtgatttta ttaattattt acaagctcgc tgggtcgatc taaatatcga agtcggtgat
     3421 gctattcggg ttaagtttca gcgtgaccgt gattatttat cgcatccctt tttaggtatt
     3481 gttattcgtt tacttgaagg agctaggatt catgtcccgc agcgcgaaga aacgctagcg
     3541 ttactaaaaa aacacacgaa ttatccgctt cataatttta ttacctttgc ctttaatttt
     3601 aatcgcaatg acaaagatgc tccttttgaa atattaagcg atgattttat taagattatc
     3661 ttttacttaa caaataaaaa aggttatccg catttagaaa actttgatag tttcttagaa
     3721 tataaattac gtattagcgg ggttagtata attaacctcg tatatgctag acgcagtgcg
     3781 cttgtcgaaa aaaatatcga caaagtaaca gaacacttat ccctagcagc aatacagtca
     3841 ggtcaagacc accgtaaatt cttaggtcaa aagtactatg aactagcgac attatttaaa
     3901 aaacacaacg attttgccgg aaaattgttg caaaaggccg ttgactttaa aaattatgag
     3961 gcactattac cacttgttaa atattatgaa gaagcaagtg acgataagga tgactatacc
     4021 gctattgcat tttattatct tgtaattgct catcaccttg aaatacctgg tgccggagaa
     4081 aaattaaaga cttactatga aaaaggaggc gtaaggcctc cgtgtaacaa gatgttaagt
     4141 aaattttttg aagactaatt tacataaagt caaataagct aatttgatta ctttctggga
     4201 gaccctctag cgctcctagt tcttttaagt tttcaatgtt tgaagtgttt aaacgtgttc
     4261 tttctactaa atcttcaatc gaaataaatt caccattctt ccgtgcttca accacgcttt
     4321 cagcagtgcc ttcgccaagt gctctaatga cgataaaggg cggaatgatt tgattatttt
     4381 catgatcaac tttaaaccga gtagcctcac tcttatatag atcaatattc gaaattttaa
     4441 agccacgatt aagcatctca attgttaact gtaagttttc aacaatatta ctgtcgcgcg
     4501 gatttaattc cgatgtttta cgctgttcct caaatttttg caactctttt aagataactt
     4561 cttcgctact tatcattgtt ttaagatcaa agttatcacc gcggattgta aagaatgttg
     4621 cataatactc aagcgggtaa ttaagtttaa accaagctac tttcactgcc ataataacat
     4681 aagctgcagc gtgcgcccgt gggaagagat attcaatttt cttacatgat tcaatgtaat
     4741 agtgcggaac accagcttca cgcatcttcg tttcaagacc ctcttcaaca ccgcgtcccc
     4801 ttcgcactga ttccatgatg ttaaaggcat ctttttcatg taaattttgg cgaattaaaa
     4861 atgtcataat atcatcacgg cacccaataa catctccaag tgttgcttgc tctgacctaa
     4921 ttaattcatc agcgttaaag cgccacacgc cagttccgtg gcttaaacct gaaacaatca
     4981 caagatcatt aaatgttttt ggttttgctt ccgttaataa ttgacgaaca aagttagtgc
     5041 caaattcagg taaacctaaa gcacctgtta cttcgccaag cggattactt tttaagttaa
     5101 gtgcttcatg acttgagaat aacgaaattg ttgcacggtc attagtgggc acatcttcaa
     5161 acttaatacc ggttaaatca caaagcatct taagtgttac tggatctttg tgacctaata
     5221 aatctagttt taataagtta tcatgaattg aatcatagtc aaagtgcgtt gttaaccagt
     5281 cgctttccgt gttccccgct ggatattgta cgggtgtaaa gtcatatact tcatattcac
     5341 ttggaattac aacaatccca cccgggtgtt gccctgttgt acgtttaaca ccagcgatgt
     5401 gataagcaag ataagcaaca ttttcattat ttaattcatc acgatatatc ccttgttttt
     5461 cgtaataatt taagacatga ccaaacgctg tccgttcggc cacagtttga atcgttcctg
     5521 ctctaaaagc attattttca ccaacaagtt ctttagtgaa gttatgtgct tctttttgaa
     5581 agtcttcagg gaagttaagg tcaatatccg gaattttatc agcttcaaaa ccaaggaatg
     5641 tttcaaaagg aatattttgc ccttcaccaa ctaaatcatg tccgcattca ggacatttag
     5701 cgagtggtaa gtcataaccc gagagtactc tttgttcatt acggaaatta ctaaatttac
     5761 aatgagggca gaagtaatgc ggtgggagcg gattaacttc tgagacttta gcgaaataag
     5821 caacgacgct tgagccaaca ctaccgcggc taccaacaat aaatccttcg tcatttgcct
     5881 ttttaacaat tagatgcgct aagtagtaaa taactgcata gccattattt ataatccctg
     5941 ttaattcacg atgaatacgg tcactaatta aagttggtaa tggattaccg taaatttcat
     6001 aagctgtttc aaacactaag ttttctaaaa gttcatcaac attttcaatt gatggtttat
     6061 ataattcttt tgtaaatggc gtaatatttt ccactaaatc attaatccgg tgcggattat
     6121 taataatcat atctgctaat tcttcttcgg taaatacacc aaacttacgc atttcttcaa
     6181 acatctcagt tgtagaacga taatgttgat cagggttttc aaaatgtgga cgcttgtcgc
     6241 gatcataagg attaagtgga tgattaacat tctttaatcc ttttgcgaca atgtaaacat
     6301 cacggaaaac tttttgactc ggtaagcaat agtgaacatc acccgttgcc acaagcggtt
     6361 tccctaattt ttgcgcagcg cttttaatat cgccaataac ttttttaaga tgctcttcac
     6421 tatctaattc acccatatta attaagtaag agtaattgct gtatggttga atttcaataa
     6481 aatcataaaa ttccattact ttaagcaagc ggtcttcatc aaagcgcgct gcagtttcaa
     6541 atacttcacc gttaaaacat cctgacccga ttagtaaatt atcacgatgg cttaaaagca
     6601 tggagcgcgg cgtttttgga atatcggtgg agaattcggt atgagacaca ctcactaatt
     6661 tatataaatc acgaagtccg tctttatttt tcacatacac cgtagcgtgc gctggccata
     6721 agtattggag cgcagcttgt gttagtttta actccgcaag ggcggcatgt gttaaatatt
     6781 gatcgtcttc cgttaatttt gtaagcattg cttgccaaat actatttaaa acttccgcat
     6841 cgtaatccgc acggtgcgcc ccgtcttcat cataaaatac ctctaaattg cgggctaaaa
     6901 cacctaatcg gtgaccacgt gattctggga ataaatagcg cgataacgct aaagtgtcaa
     6961 ttgttggatt gttaagcggg ttccgtcctc ttttacgcaa attagcgttt aaaaatccta
     7021 agtcaaactc ggcattatgt gacactaaaa tgtcatcacc taaaaactct aaaatctcat
     7081 caaggacatc atctagttta ggtttaccgc gcagcatcgc atttgtaatt tttgtaatgc
     7141 gtgtaatatg ttcaggaacg acaatacctg gattaattaa aatatcaagc cgcgctgtca
     7201 ctaggccgtg ctccacacgc acagcgccaa attcaataat ttcgttatag cgagcactta
     7261 gccctgttgt ttctaagtca aaaattacat atcgggcatt atttaaaata attggtgcag
     7321 ggttattaac gacttttaat gtttcatcga taacatatag ttcactacca taaattattt
     7381 taatatcata tttcttcccg gctttttggg cttcaggaaa ggcttgcaca acaccgtgat
     7441 cagtaagtgc aatcgcttca tggcccatat ttttagcaag ttcacaatat tcatcaatcg
     7501 tacttacggc atcaagcgct gacattttag tgtgtagatg taattcgatg cgtttccgac
     7561 cttcgtaatc atctgtcggt aatggtggtg cagcaatttc atcaatgtcg cgccctctaa
     7621 caattttagt ttgaccgcgg aaattatcaa tagcgatata accttgtaca cgcacaaagc
     7681 gtcccgtctt tagtgctttt aacacttcat cttgctttgc gccacgttta ttaatataaa
     7741 atcgtactga aataccgtga gcgtctttgc caataccgag tgtataagtg acaccgtgct
     7801 ttgtcggacg cctatcataa ctatatattt caccgtcaaa gtccacacct tcatcaactt
     7861 ctttatgttc taaatcttta atttcggtaa gattaaaagc tcctttagcg tgccttcttt
     7921 tttgccgctc tttaaccatt ttttcgtaat tttcttttag tttatcttct aacccgtcgt
     7981 gatattcaag acttctttca cagttttcat ttggatctag aggatcgcaa tctgctacac
     8041 ccgtgtcatc ttcttcatca aaatattctt catcgtcatc atcatcttcg ataaagggtc
     8101 tttcactccc aagtgaaaat tcgtcttcct cgtcttcttc ctgtgcacta ctaatgacaa
     8161 ctgtttctaa ggcgaagtta taagatagtt cttttaagag taaagaaaat tcataaagca
     8221 ccgctttagc gtcaagcgca ctaacttcat cggtaaaagt aaagcgtaat gtatcattgc
     8281 caacgaccat aataacatag ttgctttcac taagataagt atgtaaatgc cacggtttaa
     8341 aaagcgctaa agcatcttca atacgcggta atttagtgta agcataactc actttatatg
     8401 cataaggaat actcattaac gcatcactaa aatctagata gtcttcaaaa ttccacggtt
     8461 tttcttttaa aagtgtgtag tgccacactt catcaacttt tttccattct aaaaacgtta
     8521 acccaatttt cttgtcgttt tcataattaa ttgacgcaaa aaaacgagca ctaacgtttc
     8581 ccatctataa aataactcct acgaagccaa aaatatcttt cgctaaaata acaagtgtta
     8641 agccaatcaa aagaattgca ccaatcattg aggcaatttg tttaaattta ggactaattt
     8701 cttgacgagt aatcgcttca actgttacca ctaataagtg ccatccatca agtggtggga
     8761 acggtagtaa gttaaagagc gctaagttaa tactaattaa accccataag aataaataag
     8821 gtgctaaacc atgattctta agtgtttgac tagaaatatt aaaaatcccc actggtccac
     8881 taacattttt aagaccatcg ctaaataatg acttgaggcc gcgcgctact gccgtaacac
     8941 tacggataaa atctttattt gcggcggtga atgtttcact aaagttattg cgatatttaa
     9001 atttcgttaa attaaaacca gcggacacaa atttataccc gtctgttaat ttatcgggat
     9061 tttttggttc agtactaagt gttaatactt tttcaatacg ctccgcagtt tcataagttt
     9121 caccgcgtaa gaaagttgcg gtaaaggtta acacatctgt atcttttaaa tcagtgtaaa
     9181 acgaattttc actcgctaaa tcagtcgcgc caatttttaa aaggacaagc gactcaccac
     9241 caaaagttaa gtcatctaaa ctctttggtt gaaataaaac ataatattct ggttcaagtg
     9301 ctgcagcgtc aagcttccct tcaccgaagt actgaatcgt ataaccatca atcttcgttt
     9361 ctttaaaatc aatgcggtca tcactgcgta tccccattgc atatacagga ctatcttctt
     9421 taacttctaa acgaattgat aacccggttt gcggtaaaac cccattagcg aataagaata
     9481 tagcgtagcc taacactaag ttcataataa tacccgctga cataattaat gcccttttgc
     9541 cacgattaat gccgattaaa ctccgttctt tgggaatttc ttcatttaca aattcatcag
     9601 gcacagcatc ttcttcgccg agcatcgcaa catatccccc aagcggcaat gcgcgtaatg
     9661 aataataagt ttctcctttt ttcttgcgga aaattttagg accaaaccca atcgcaaatt
     9721 caaagacata cactctaaat atcttcgcag caataaagtg gcctaattcg tgaatggtga
     9781 ttaatacacc aagcattact aaaaataaaa taatgtttaa tattgtactt aaaaattcca
     9841 tactaacttc cttctagacg agtatactcc atccgttatt tattaagatg attagcattg
     9901 ccgtcacaat actaaccatt aaaagtgaat caacacggtc taaaataccg ccatgaccaa
     9961 ctaaaatcga accataatcc ttaatcgcta aatgtctttt ggtagcgcta aagaataagt
    10021 caccaatatt tgccgcaagc ggcattaaaa ttgagatgag taataaataa taccactctt
    10081 tatgactcaa aaatggtaaa accggataac caatccacga cacaattaag gcgaataaaa
    10141 aactaacaac aatcgaaaag aaaacaccac cagcaaagcc ttcccatgtt ttatttgggg
    10201 aaatccgtgg taccatctta tttttaccaa ataacacacc gataaaataa gcaccaatat
    10261 cattcataat tgttcctaaa attacataaa tgataaggaa tgcgctcgta ataatattcg
    10321 tgttaaaagt aatgtcagga cgatcagcaa tatattgcgg ataaagcctt aagaataaga
    10381 aagcttggaa cccaaacccg gcaaagagca ctaacaagaa taaatacgcc gcgatatcaa
    10441 aaccaagatg cggatcaaac acaattaaga taaatagacc aattaaaatg gtcgcaagta
    10501 agaaacttga tacttgaatt gattcaaaac cgttttccac cacccacttt gaaatatccc
    10561 accacgagta tccggcctta atattagcca ttatattatt tttaataaaa atccaaaaga
    10621 taagggcaac agttgaaaca atcgctaaga tatacacaca ccagtagcgt tttgatattt
    10681 tcgtgccacg cataatttca agcacagcaa taattgacac aaagaacaca agcgctaaaa
    10741 agtaccaccc acctaaaagc aaggcaggaa cagcgattac cgctaaaata atcgaagtaa
    10801 ttgtgcgggt cttcattgac ttctttgttg cttcactaat ttcatttagt gatattttaa
    10861 tcgttgaagt ccgtttggtt tttggtttaa tttcattagt ttccatcact ataatcctcc
    10921 aaacctgcgc gttctttttg cgtattcttc taaatcacgg acgagacact cttcgtcata
    10981 atccggccaa taagttgggg taaatataaa ttcagcgtag gctagttgcc acagcatata
    11041 attactaagg cgcatctcgc cactcgtccg cactaaaaga tcaaccggcg gcattccgct
    11101 tgtatctaaa taattttcaa aagtatcagg tgttaatgct tgaatattaa cactttcatc
    11161 tgctgctagt cttttaatcg cacgaacgat ttcatcttta ccaccataat ttatacaaat
    11221 atttaatgtg gtgcccgtat tattttttga ttcttctttt gcgtaattaa tcgcttgttg
    11281 tgttgattca ggtaatcttt ttaagtcacc agaagtcaca attttaatat tatgtttttg
    11341 tactttcttt aacacactat gtaatctttt ttcgagtgtt ctaaataaaa aatcgacttc
    11401 tttttttggt cgtgaccagt tttctgtaga aaaagcgtat acactcataa aggggatatt
    11461 acgtttacta acagcaatac caacatcaaa aagactggaa aaaccttttt catgaccata
    11521 agttcttggt cttttacgcg cctttgccca tctaccatta ccatctaaaa taaaagcaat
    11581 atgtttaatg ccataatttt catagtttgt aatcttcgtc ttactcatat gttaaattat
    11641 acaataagaa taactatttt cattaaataa catttttaaa atgttaaaaa agaaaagcgt
    11701 ttgtaaaaat aatttatttc actaatagct ttaataagga tacaacggat aatctttatc
    11761 aaaagatggg aaagagatcg ctactttttc attaaatttt atgattgtat cagtatagac
    11821 ttttcgcttc ttaacccaca taagccgcag gtcttcttcg ctagaataat aatgttttcc
    11881 gcgatttact tattactttt ggctcttata ttaattttag gataacacac tagtgtgtta
    11941 caaaagaaaa aagccttgta aaaggcttaa ttcatagttg ttaatttaca ttaaacggtt
    12001 aagatttctt cttctttttc tttaagtaaa tcatcagctt ttttgacata ttcgtcagta
    12061 actttttgca cagcttcttc tgctcttttg ctcatgtctt cacttaaatc tttatcattt
    12121 ttaatactcg tattagcgtc acgccgaata ttacgaatgg caatttttgc ttcttccgta
    12181 aactttttag cttgtttcac taaatcacgg cgccgctctt cggtaagtgg gggtaagatt
    12241 aagcgaattg tatcgccatc attaatcggg ttaatcccca gtgttgaggc gttaattgcc
    12301 gctaaaattg aacgcatatc acctttatca taaggcttaa ttaaaagttg acgcggttca
    12361 gggacactaa ttgaactaat ttgattaatt ggcatttttt caccgtaata atcacattca
    12421 atccggtcaa gtaacgccgc acttgcccgt ccggtgcgga gtgttgaaaa ttcactttgt
    12481 aaattcaaaa ttgatttttc cattcgctct tcagcgagta atgcatattc atccatattt
    12541 tttacctctt cgttataatt gtaccaattt ccatatgttc ttcaagtggt tcgaagttta
    12601 aaacttcatc cataccaatg acaaaacttg tgatgttcgt tttatttagt acttctaggg
    12661 cgcttaaatc catcgcttca actttaagat caattagttc ttggtaagtg acttgggtta
    12721 ttttttgtgc atctttatat tgatgcggat ttttaatgta aacagcgtca acaccgtctt
    12781 ttacgaaaat aattagatca gcattcatat caatagcgcg gagtgctgca cctgtatcag
    12841 tcgtgtagaa cggtttacct gtaccaccac caaaaatgac aacttctccc ttcgctaaat
    12901 gtttttttgc taattctgga ctagtttttt ccgcaagtcc ttcaaccgca aaagcgttta
    12961 agacaacagc aggtaaatca ttttgcgtaa aataatcttt cagcgctagt gagttcaaaa
    13021 atgtcgcagt catccccata taatcagcag cagcagggtc aaaaccaata agttcagcgt
    13081 ttttcccccg ccaaatatta ccagcgccaa caacgatacc aatttcccat ttttgatgat
    13141 ggcggaatga tttgacaaaa tttaaaatcg cggataatac ttttggatca taaattccgc
    13201 tttttttatc accggcaagg gcttctccac ttagttttaa gagcactctt ttatatacca
    13261 tacttttttc ctccaaaaaa acacccgaag gtgttttctt tttgtggttt attatttaat
    13321 ttgaccaaag acttcagcgg cgaaatcttc ttgacgtttt tcaattcctt caccgacttg
    13381 gtaacggaac atcactaaga ctttattgtc aagatctttt agggcttgac caactttctt
    13441 tgtatcgtcc attaaatatg tttgttcggc taagactaaa tcactaaagt attttctaat
    13501 tttaccttca acgatgtttt taagcatttt ttctggtttg tctgctaaac gtggatcgtt
    13561 tttagtaatt tcaagttgaa taccgcgttc atgattgtaa tcttcttcgg taatatcatc
    13621 agtggtaata tagcttggac tattcgcagc aatgtgcatt gttaagccat gcgctaattc
    13681 gtcatttttc ttatctaaga gtacgacagt agcaatttta ccacccatat gaatgtaagt
    13741 tgcaccacgg tcttcaacct taacgagttc aaaacgacgg aatgataatt tttcaccaat
    13801 ctttactgtt gcttcaacta aaagtggatt aataattgca tccgctgctt ctttattagc
    13861 aggtttatgt ttgagcaata aatcaagaac agtgttcact aacgctttaa agtcatcacc
    13921 gcgactaaca aagtcagttt cacagttaac ttcaacaata gcagcgtagc catcttctat
    13981 ttttgcaagc gctaagcctt cagcagcaat ccggccgctt ttcttcgcag cggtcgtgat
    14041 accttgctca cgaagccaat ctttagcttt atcaacatct tcaccgtttt caacgagggc
    14101 ttttttacag tccatcattc ctgcgccggt aagttcacgc aattctttaa ttttatcgat
    14161 taaacttgcc attaattatt ctccttcttt ctcagttttt acttcttcag ttttcacttc
    14221 ttcaactttt ggagcttttg gagctttagc ttttggttca gctttcactt ttggctcttc
    14281 agctttttca gctttcggtt ctttaacttc tttttctttt tttggtttag ctgcagcagc
    14341 aggtttttcc gctacttctg gagcagcttc accttcttct ttagcttcag ttttacgaac
    14401 acgtgttggt ttatcgctgt gttgggtctg cgctttgcga acttgacgcc gttcttcgga
    14461 gtcagtgcga cgggcacgtg gttcgttatg ttttaacgct tcttctaatg tttcagtttc
    14521 aggttcagca tcgcgtgtat atgctaaaac agggatacca ccttttgctt caacaatcgc
    14581 gtcagcaaat aaagtaagca ttaattgcac ggttttagta gcgtcatcat tacttggaat
    14641 aatgtaatca acgacatctg gattacagtt tgtatcgaca agtgcgaaca ctggaatacc
    14701 taagttacgt gcttctctaa ttgcgtttgc ttcgtcaatc gtgctaacga caaacactgc
    14761 ttctggaact ttgcgcattt ctttaatgcc ttcaaagtta tcatttaagc gtttcatttc
    14821 tttacgaatt aagattgctt ctttcttcgg tagacgatca aaagcacctt cagcttcttg
    14881 catttctaaa tcacgtaaat attttacgcg agttgaaatt gttctaaagt ttgttaatgt
    14941 accacctaac caacgattat tcatatagaa tgaaccactt cgttccgctt cacttttaac
    15001 gtgttctgca tgttgtttac gggtgccgac gaataagact ttaccaccgc gagtaacaat
    15061 ttcgcgtaat gcagcgtaag cttcttcgat tttgacaatt gttttttcta agtcaatgat
    15121 atgcacattg ttgcgtgaac cataaatata tggtttcatt tttgggttcc agaatttggt
    15181 ttggtgacca aaatgtgccc cagcttcaag taattttttc atggtaatga ctggtagatt
    15241 ttcatctggt aagacatttt ccattgcggg agttacttcc tcctcagtta atacgacttt
    15301 ttcttcttca ctcatgtttg agcctccttt tgttatttcc tccacatttt cgaaccttgt
    15361 ccctacacct gtagaaccgg acgaggaatt caatgtgtgc gtattagccc ttttttgggc
    15421 cattagtata ttaccatagg taaaaggttt aggcaactag ttaacttata aagttaaatg
    15481 aagtgattag tctattttct ttttagcgcg cggatgggca gtatagtact cttttgtaat
    15541 tttttcttcg ctaacatgcg tataaatttg tgttgtgttt attgactcat gccctaataa
    15601 ttcttggatg acccgtaaat tagcgccttt ttctaataaa taagtcgcaa aagagtggcg
    15661 gagtaagtgc ggatgtaagt taacatactc ccccgtcttc ttttcaatcg aaagcaatat
    15721 atagcgtaac ccttggattg ttaatttatt accttgggca ttaagaaata aaatgtttgt
    15781 cggttgacta ctttttcttt tcgctaaaag ttctttgcgt aaaccattta aataattgcg
    15841 taataagact tgtactcggt ctgtaaaagg aaccattcgt tctttcttac ctttgccaag
    15901 taccattaaa aagcgttcat ttaagttaaa agattgaagt gttaaattaa taacttcact
    15961 agcacgcaac cctgttgcaa acattaattc aataattaat tgatcacgat cccttaaata
    16021 atcagtgcgt ttttcattag cgagtaagat taagcgaatc tcttcttcat aaagcacgcg
    16081 aggcaatgct tttgttgctt ttggtgcttg tactgttaaa aagggattat ctttcacata
    16141 gcgcttatct tgtaaaaatg aatagtaatg gcgtaagctt gctagtcggc gctggatgct
    16201 gcgccgcgat acatatttat caatttgatc ttttaaatat aaccggatta atggtttatc
    16261 aacctcgtcg tcacgcatat tctgattagc gagaaataaa taaaaagttt caatgtcact
    16321 tatataagca atgactgtgt tttctgaaaa gcgtctagtg taacgtaagt aatcttgaaa
    16381 ctcttgtaaa ggtctattca tttaatgact caatgtactc cttatatttt tgcgttattt
    16441 ttaatgattt ctcggcaatt tccatgcggt tttgtttagt agcacctcga actattccaa
    16501 aattagcgtt cattggagca aagttttctg gattggcgtt atttaagtaa tgcattaaac
    16561 taccaagcat tgtctcacac ggatactcta attttacacc ttttatttta gcgtaaacat
    16621 taagggcaac ataaagcccg ctagcggcac tttcaacata accttcaacg ccactaagtt
    16681 gaccagcaat ataaatatca ttgttaactt taagtgactt atctaaattt aggacacgcg
    16741 gtgcattaac aaatgtattg cggtgcatta atccgtaacg aacaaactca gcgttttcta
    16801 aaccgggtat aagtctaaat acacggcgct gttcagcata agttaagttt gtttgaaagc
    16861 cgactaagtt ataaagcgag ccaattacat tatcttgccg aagttgaagg acagcataag
    16921 gtttatgatg ctcatcttta ccaagtccgc gtggtttaag tggtccaaag cgtaaggttt
    16981 taggtcctct cttcgctatt acttctaatg gcatacaggc atcaaagtat ttcgtatcca
    17041 tttcatgaat tggcgctagt ttagcgttaa ttaattcatc ataaaatcga taatattctt
    17101 cttttgtaaa tggacaatta atataagcat cgtccccttg atcatagcgc gatttataat
    17161 aagcgatatc catattaatt gaatcttttt caatgattgg tgcactagcg tcaaaaaaat
    17221 gtagaaacgc tgtatcgata agtttactaa tcgtgttctc taagccttta ctagttaaag
    17281 gtccgctggc aataataaca ggaacatgag aaggaagttc gactacttct tcgttcacta
    17341 ctttaataag cggatgagta gtaataatcc ttgttacttc ttcgctataa agttcacgat
    17401 caacgcaaag agcattaccg ccagggacac taacacgcgc agcaacactt gtcattagtg
    17461 aattatatat ttccatttct ttcttaagta aaccgcatgc gtttgtaagt tctttactct
    17521 ttaatgaatt agaacacaca agttcaccaa gcttcgcgct atgatgagca ggcgtcatga
    17581 caccaggacg catttcatat aacttaactt taatcccttt ttccgcaaga aaaaaagccg
    17641 cttctactcc tgctaatcca ccaccaataa ttactacttg ttccacaatg agattatagc
    17701 aagaaaacgg caatttccct acaaaaataa gttatttttt acgtctagta cttttaatag
    17761 ttgcggaata atcacaactt ttataatttg agcaacgaat atatttacga ttacgtgaat
    17821 cataacgata agttaaaggc tcattacatt ttggacaatt ttcaccagtg tattcaacaa
    17881 tgaattcggt gtatttacat ttcgggaaag tttcgcagcc aataaagcga ccatatggtc
    17941 cttcgcggta cactaagttt gagccacacc ttggacaatc gcgaccgacc ttttctggcg
    18001 gtagtttctt cattcctttg cgcgcatcat caattttttg actaaagtca ctgtagaaat
    18061 cactaagaaa ttcaacttta tcgccttcgc catttgctat tttatctaat tcattttcca
    18121 ttgaagcagt gtaatcacta ctaataatac ttgggaaaaa ttcgctaaga gcatcaattt
    18181 gtctaaagcc aatatccgtt ggcttaagcg tacctttaag gctagataca tatttacgtt
    18241 tttgtaatat ttgaatcgtt ggtgcataag ttgatggtcg accaataccg agttcttcca
    18301 tcgttttaat taagcggcct tcactgtaac gcgcaggtgg ttcagtaaat ttttgttcag
    18361 ttataacatc aataattggt aattcactac caacatgcat ttgcggaagc gctgagtcat
    18421 ctactgtttc ttcatctttc ccataaattt ttctaaagcc cgggaatatt aagattgttg
    18481 atgtcgcatt gaaaatagta ccatttgaat taaatgtata acgcgtaact tgttctttac
    18541 taccgaccat taaacttgca agtgctcgtg tgtaaataag tgaatacaaa ttatattctt
    18601 ctctcgttaa tgaatcttta attgaatcag gagtaaagtt taagttcgtg ggacgaatcg
    18661 cttcgtgagc atcttggaca ttttgtagtg ttttaccacg cgcttgagta cgaccaagat
    18721 attcggcccc atattcttta acgatgcgtt gtgatgcatt agcgacaaag cttggcgcga
    18781 gtcttgtcga gtcagtacga atataagtta ctaaccccgt ttcaatcccg ttaatttgta
    18841 taccttcata gagtttttgg ataatccgtg aagtgcggga agcactatat ttaaatttac
    18901 tacttgcttc ttgttgcaag gttgatgtgg taaaaggcgg cttaggatta gtgtgtttaa
    18961 tttcactttt aacatcagtc acaacaattt taggcgctag tttactaacg atatcatttt
    19021 tcatcgcttc gttaacaatc ttagtatcac cagcgtaact cttattaaaa ttcacactaa
    19081 aaagagcatc gtcatattta gtgtcaattt ttagtgtcca atattcttca gggacaaaag
    19141 cagtgatttc gttttcctta tcaacgatta gttttaaaac cgcactttga actcgcccag
    19201 cgcttttaac atttattttt tgatttaaga gactagacag ttcaaagcca acaatacggt
    19261 caagcattct tctagcttct tggctattaa aaaggtcttc attaagcaag cgtggcgcag
    19321 cgagggcatt agtaatcccg tcccgcgtaa tctcatgaaa ctcaagacgc ttagtcgtat
    19381 ttaaatcaag gtttaaaagt ttagcgatgt gcaccgcaat tgcttcacct tcacgatcag
    19441 ggtcagtggc aaggtagact tcactacttt catttttagc tttttttagc gtatctgcaa
    19501 ctttttgccg actcttatct aatacatagt caccttcaaa agtttcaaga ttaataccta
    19561 aattcttgcg accacgtgtt gaaaggtcga cgatatgacc atagctagcg agtactttgt
    19621 actcgtcact taagtagcgt tcgatcgttt taattttgct tggggattca acaattacta
    19681 gtttcacaaa gcctcctatc ggttataaaa tatagagggt ttttttgcgc ttgtcaaatg
    19741 taaaactttg tgaattttct ctttatttat aataaataaa acatttttta ttttcttagt
    19801 ttggccagag ttttataatg tcacttgcgc tctcgacaag ataagcacca tcttttatca
    19861 gtctattaca gccactatta cggccagcgc gctcaggaac acagaaaaca tcactccctt
    19921 tttctaaggc gtaaccaacc gttattattg tgcctgagcg atatttagct tcgatgatta
    19981 aaacggcttt tgtgagcgcc gctacaagtc tattgcgaaa tttaaagtaa tctggttctg
    20041 gctttgtatc attaggatac tcagttatta aaagaccgtg cttactaact tcgtcatata
    20101 gttttttatt tgatttagga taaggataat taattccgct gcctaggacc gctattgtgt
    20161 tgccacctgc ccgtaaacac gcatcatgac taaacccgtc tataccgcgc gctaagccac
    20221 taacaatcgt aaatttttct ttaacgagtc cttcgacaat ttcataagtc atgtttttac
    20281 cgtattcact atactctcga gcaccaatca cagcgatagc tttttccaaa ttaaaaagta
    20341 attctttgtt accttttaaa aataaaacaa ttggcggatt attaactgtt ttaaaaagcg
    20401 ctgggtagtc ttcatcaata atcgttatgg tatcaacttt ttcccagtct tctttatatt
    20461 tattaaataa agcgtcatca actggttctt tggagcggag tgcattaata attgcgtggt
    20521 aattgccttc atatttactg gcaaagtaaa gtaaaacatc gcgtgctttt ttattcattt
    20581 gtagttcctc attatataaa taaggaagaa cttaattttt tatcacatta aaataattta
    20641 atttgtttat tgagcgcatc ttgcactggt ttataagagt agcgatgcac atctttaatt
    20701 gggccataag tatcaagtgc ttgtaaatgt ttccgtgttg gataaccttt attattttca
    20761 aaatcatatt gcggatagag tttcgcgagt ttattcatat aagcatcacg cgctacttta
    20821 gcaagaatac ttgcggcggc cacactaagc gaagtcgcat ctccttgaat aattgcttcg
    20881 taccccactt ctaaattaga tagcggcatc gcatcagtta acacaaaatg aaattcatga
    20941 cttaagccag taactgcttt ttgcattgct aaaattgttg cttgataaat attaacttga
    21001 tcaataacgg gagcgtcaac atgcacaatt tggtaatcaa gagccttctc tttaattaag
    21061 tcacttaatt caagtaactc cgttgctgtt aattgtttcg agtctttaat tcgctcatcg
    21121 ttaaaaaagg gcggcaaaat aaccgcggca gcaaacacag gtcctgctaa agggccgcgt
    21181 cccgcttcat caactcccac taaataaagg tgtccgtgtt ttcttacatc attatcaaat
    21241 tcaaaatatg tcatattatc actccaaact tacaagctag taattcaatt actaacatta
    21301 ttatattata gaaactaact atttctattt ttaatatttc aaatatttgt gattttgttc
    21361 ctaaaatgcc taagctagta ttgagatttc atttttaaaa attaaaatga aaatatagat
    21421 tagtgaaagg agaagattat gaaaaaacga aaatttttac taatcccact acttttagga
    21481 ttgctcttaa ctggatgctt tggtggagac ggtcctgaag atattattga tggcgacgat
    21541 aaaagcagtc aaaaaactac taacgatgta gcggaagaag atattcttga aaatatggat
    21601 tacgaaaaaa gagaaggctg gccaacgcaa aagcttgcta ctttcttatc aagcgaaaga
    21661 gttaatgaaa ccgttccagc attaacaaat atcgccacta cttattatca tgaaaataat
    21721 gacgctaata ataagtactt tgcgattgta gtacctggtg ctaatcgcga agcagattat
    21781 aaagccatac ttgaaggagc tggctacaca tacagtaaac ttagttggac tagtattgct
    21841 gttaacaccg aagacacaat taatattgcc taccaataca tgcaagatca tccagttctt
    21901 gattacgcta tgtatattgt tattagtgcg cttaaggctg attcatttcg ctttgataag
    21961 cttgaaagtg atatcggatg gccggccaat caacttcaaa ctttcttaag taaatacggt
    22021 attaccgaaa gagttcctag tcttactggt ggtaactatt tcgctgaatt aacagagcgt
    22081 aatgaattta tcgtttgtgc gcctggtgtt aattttgttg atagctattt cggcagtctt
    22141 gaaaatgaag gttatacagt aaatgggtca aacgctgtaa gtacttcagg caaagtgcgg
    22201 attaatgtga ccgctaacga ttatccgttc ttcttctttg atgcgacaaa aattgtcata
    22261 aaacctgtgg tctaaaacta aggtctcgcc tattaaaaag cgaccgttat ccactagcgg
    22321 tcgtttttta tatttcacta atttactact ctagactaat tcggccaatt agcccatttt
    22381 taaattcatt taagattaag atttcggtgc gatcactaac atcaaaatct tcaccaataa
    22441 tcccgcgacg cgtagcaata gcactaatca cggtttggta gttactatct tctttaatat
    22501 caatatcgta aaaagctacc atttcttcaa agtaatatgt ttttaagtaa tttactaaat
    22561 actctgccat tgtatgcgtt ggtaaaatct cttgtctaat tgtacctata agcgctaatt
    22621 tcatgcttgt atcttcttca tcataattag gtgataaaat tcctggtgta tcaattaagg
    22681 caatttcttc actcactcta atatactgat tagcgcgagt atgccctggt ttattttgca
    22741 cacttgcggc tttgcgtccc gctagtttat taataagtgt tgatttacca acattaggaa
    22801 tcccgacaat cattaccgct aaaggtcgtg gttttaatcc gcgacgcaca tcacgttcaa
    22861 ttattggttg cccgatttct ttaatctttg ctaatatttg tcgcacaaat ttccgttcat
    22921 ttaaattacc cgctaaaaca tttaattttt ctgtatgttt acttactctt ttaggatcag
    22981 ctaagtcggc cttagttaaa acataaagat ttggctttcg ttcaataatc gccgttaagt
    23041 aagtagcgcg actactatgg gggatccgtg catcgcctaa ttcaacgata atatcgacca
    23101 tctttagcgt ttcaatgagc ttgcgtgtcg ctttttgcat gtgacctggg aaccaaccaa
    23161 tttgtttact cattagtagt accgccattt ccacagcaca taataatgct gaccaataaa
    23221 tttaacttta cctgattcac taacttcaac attttccgta taaccttgaa ttacgactaa
    23281 tttgccgctg atattgtcaa aatgaacagg accaattgcc cgtgaatcac tggaatttgc
    23341 acggttatca cccattaagt aataagtttt ctcttctaaa gttaacggtt ctttaagtcc
    23401 gttttcatat tttggagaac tatcgtttaa atatgttgcg gttgtataac gattacttgt
    23461 tgataagtag ggctcatcga gtttaaattt ctcggttgct tcaggcagtg taataaaaac
    23521 ttcgtcccct gtcgggttat caataatttg aatagtttct agtggcaaag caatcacacg
    23581 cttaataatg ttaataccgt tacgccgaaa tacaacgata tcaccacgct taatattgtt
    23641 gcgggctttt ggtctttcgt ccattaagcc aaattcataa tagcggtgat gaaagtcatt
    23701 gagtgttggt tgcatacttg gtccatcaac ataaactggg gtgtaagtta aatcaacata
    23761 agtcacaaaa agcgaaacaa caatagcggt cacgagtaaa atagtccacg tcacactaag
    23821 cgctttacgt agtctgggtt tatttttccg atttatctct gcttcactta tttcttcatt
    23881 aataagtact tcttctatga ccatatagta attatataac gaaatgtaca tttgatgtac
    23941 attttcctac ttattatcac aaataaatac tcgttttata agaaaaaaag ccagttttct
    24001 ggctttctct cataaatgat tttaacttat ttcttctctt taattcgggc tttacgacca
    24061 gataatttac gaatgtaata aattttgttc cgacgaactt tcccgcgacg gagaacttcc
    24121 acactgtcaa tgactggtga atgagtgaag aaaacacgct caacaccaat accacttgac
    24181 atcttgcgga cagtgaatgt gtggttgacg cctgcgcctt gcatagcaat gaccacgcct
    24241 tcaaaaaatt gaatccgttc tttgtcacct tcacgaattt taacagcgac cttgacagta
    24301 tcaccactac tgaattcggg aagatcggta cgaagttgag aagcgttaat ttcattaact
    24361 aaatttttat tcatgaataa ctcgctcctt cctgcaatat tattttcgtg ttcttacctt
    24421 ttggcagcgg accacagcga ctttgcgctc cattaatata ccaaaagact actcttatgt
    24481 aaagagtaat tactaaaatt ttaaaaaagc ccactctttc gtaaagtggg ctattagcgt
    24541 ttattaaact tatgtttaaa ctaaagcttt aaggataaag tagataacaa atactcccgc
    24601 tacaacatac atcggtattt taacttcttt accacgctta ctaacaaaca tcataatgac
    24661 atacattata agtcctaaac ctaagccatc actaatcgag taagtaagta aaataagcat
    24721 aattgtaaag aaagctgtaa aaccaataat gcgatcatca aagttaacat cttttaagtt
    24781 accaacaaag attaagccac caacactgat aagtgccata cttgtaaccg ctggttcggt
    24841 aaaaatataa aagattgggt aaataaaagc actaagtaag aacataaatc ccgctacaac
    24901 cgccgctaaa ccagtcttag cacccatttc aacagcaatc ccactttcgg aaaatgaagt
    24961 tactgtactt gtaccaagcg gtgcacaaat aacggcacca aaagaatcag cggccactac
    25021 tttatgacca ccaaccatct tcccatcagc gtcgataata ccagcgccac gtcccacagc
    25081 taaaattgtc gctgtcgtat caaaaagatt aacgagtact agtgagaaga taaaggcgta
    25141 tgttgaagga ttgcttaaag cagtgcccca tgtttcttta tcgaaaatct taaaagcgac
    25201 tttttcaaaa ccttgtccac cccatgaagt tggattacta aacatatctt taatattagt
    25261 aaaatggaaa gcaattggac gcccagcgtc agttgcttct ggtaatgcaa agatattcca
    25321 caccgcgaca gaaacaacaa caacaatgat cattgcaatt ggtattgcga gttgatttaa
    25381 ccgcttattg ctggaaaaca taaagataac aacaagtaaa acaccgaata aagaaagtaa
    25441 gaccattgga tcacgtaagt tcccaagtga cactaaagtc gcaggactat caacaataaa
    25501 accactgctt ttaaagccga caaaagcaat gaaggcacct agccctgcgc tgataatata
    25561 tttgatgtca ttaggaatcg catcaatcat catcttccgt aatggtgaaa gactaatcac
    25621 cgcaaaaata ataccggcaa aaaacatcac aagcaaggct gcttgccatg aacctagcgc
    25681 tccataaact acgtaagcta ggtaagcgtt aacccccatg cctgcactta ggacgatcgg
    25741 gaaattaccg taaagtccca tcactaaact aacaatagca ctaacgatgg cagtagcaac
    25801 gaagactccg cttttgtcca tgcccatatc acttaaaata ccagcgtttg ttgggagaat
    25861 atatatcatc gctaaaaagg tgactaatcc tcctaaaatt tcacttgtca agtttgcttt
    25921 tctttcttca aacttgaaca gcttatttag tagatttttc ataaattgct ccttgttttt
    25981 aatataccta ttttagtcaa ttttaagaaa aataaaagat atacatacta atttaactaa
    26041 agttaagtaa aattacttga ctaagtaaac gagtcacgct aaacttatca cggtataaaa
    26101 aggaggatta cttatatggc agttaaaatt agattaaccc gtgttggccg cactcatgat
    26161 ccacattatc ggattgttgc cgctgatagt cgctatgccc gtgatggccg tattatcgaa
    26221 caaattggac actatgatcc aaagaaagat cttaaagaag ccgttatcga tcgcgaatta
    26281 gcgcttaaat ggttaggaaa tggggcacaa ccaagtgcaa ccgttaaagc tttattccgc
    26341 gcccaaggca ttaacgaaga attcgcaaaa tcaaaaaagt caggtaaata attatgcgtg
    26401 actacgaagc attagtgcgc acgattatta ccccattagt gaaaaacgaa caagctttac
    26461 ttattcgtca aactaacgaa ggccatcgta ttattattaa catctttgcc gaaagtgatg
    26521 atattgccgt cttaattgga cgtcgcggtg ttgtcgctaa tgcattacgt gaagtagtgt
    26581 ctgtcgccgg taaacttgac gaagaacatg tcttcttacg ttttgaagca ctagacgaaa
    26641 ataaataact ctcactcgag agttttttta aattgatata ataaaattat gaacaaagat
    26701 tatttaaaaa taggttatat tgttgaaacg catgccttaa agggtgcact taaaattaaa
    26761 ccgactactt cttttgcgga ggaacgtttt gaagttggta ataaagtatt agtaaaaaat
    26821 aatactactt ctaaaactga aacattaacc attcaaagtg ttcgtgaaca aaaaggacta
    26881 ttaatagtta cctttgccga aattgataac atcaatattg ccgagaatta tctaaaagga
    26941 tttcttttca tcgctaagaa agacatcacc cttgaagaag gttacttttt ctttgatgac
    27001 cttttaggga tgaaagtcgt tttagaagat gaaacacttg tgggtgaagt tattgaaata
    27061 cttgaatacg ctgcttacca cactttaaga gtaaaaaggg aagacggtag taatattcta
    27121 attccatata tcgaagaatt catcgtttcc actgatttag cgagcaaaac aatagtattt
    27181 cgaccaattg agggtatgtt atgaagatta caatattaac attatttccc gaaatgttta
    27241 ccaatttcct tgaaacatca attattaaaa aagcgagact tcaaggccat gtaacttttg
    27301 atattgttaa tatccgtgac ttcgctgata acaaatggaa tagagttgat actccacctg
    27361 tcggtggtgg agcagggatg attcttcaag ctgaaccaat tgttaaagca cttgaaagtg
    27421 ttgactctgg tcataagctg ttactttcac cgcgcggcaa tgtccttact aatatgaaag
    27481 caacagcgct aagtcaaaaa gaacatttaa tctttatctg tggccattat gaaggaattg
    27541 acgaaagagt gaataacttt atcgatgaag aaatatcaat cggcgactac atcttaactg
    27601 gcggtgaact tggcgcgatg gtcattagtg acgctgctat tcgtttactt gatgaagtaa
    27661 ttagcaccga aagtttagat attgaaagct ttgattacca tttacttgaa tatccgcaat
    27721 atacggagcc atatgacttt agaggatata ctgttcctac tattttgtat tctggtaatc
    27781 acgcagcgat tcgtaagtgg cggctaaaag agagtttacg tattactcgc gaacgtcggc
    27841 ctgatctttt taaaaagatt acaaaagata acgaaattaa aaaactgctt aaagaaattg
    27901 atgacgacgt tattggtgaa tgggagaaag acgctattga aaaagggtca cgcttcacaa
    27961 aaaacaaaac ttagaaaaaa agataattta taaatttaca aaataggcaa taatacttct
    28021 gaaaaacacg gaataaatgc ctataataaa tttgatgaga aataggatta aggaaattta
    28081 actattaata aatcaagtta aatgataaga gcatagtaaa aaatattcta aacatagaat
    28141 tattttcaca aaattgtatt gatggtgaag aaataccaaa tatacaaaaa attaaaaata
    28201 ctgtttacca actagtttct acatttggct gggaaacctt agtagcggag tgaatgaagt
    28261 atttaaaaca tgaggtgcat gatagaaaaa cagcatttaa ttttgctacg tggttgttta
    28321 tttatgattt tggtaaatgc gatattcgta atccctatcc ttttttagca cttcttttca
    28381 aaaaaatgga attagataaa gacattcaag ataatctcaa taatagattt gatacaccat
    28441 atgaccggtt tttagattta tatgttgatg ttcttattag ttctaaaata attaatgcaa
    28501 acgaagcaga ttatttgatt cctgacaaag acactatgct tttagatgaa cttgaaaaat
    28561 tataaacata aactttcata atctatataa attgcatact aaattagcaa gaaaatagca
    28621 acaaaaatta tagctttgca cgccgctatt cttacatcga aagtcctctg tctgaatgat
    28681 aataatctag ggggttgtat gattgaacga ttacactcga aattgtatta attaggacaa
    28741 ctcataccat ctagcttagt ctgttttgat acaattatta atgcattttt taattatgaa
    28801 aagactgaaa atatggtttt tcgcgataat tgaactaaaa ttaccatttt ttaagctgct
    28861 aaacgaagtg gaatcactat gtaattatta tataaaatta aattaattga gcaattagcg
    28921 gcgaaggtac taaaaaggat tcttgctttt cttgtatcga aatgatgtta aaaacaatca
    28981 acttgttgac tatttttgac gtcaatgtta aaatacacgt gaaaaacagt caacattatg
    29041 actttatttt aggagttggt aatatgataa aaagagattt ctatattaat aagttaataa
    29101 atagtaaagg caacggaaaa gttaaagtga taactggttt acgaagatgt ggtaaatctt
    29161 atctattatt taaactatat aaagaatatt tgttaagcca aggtataagt gaagatcaaa
    29221 ttattgcata tagtttggaa gggtatgaaa atatacattt aaggaatata aatgttttat
    29281 atgcgagcat taaagcaaga ctaaaagatg ataataaaaa atattatctt ttcattgatg
    29341 agattcaaaa cgttcatcca atgcaaaatc cttatgttga atctaaagac ataataactt
    29401 ttgttgacct tatgttagag tttgtaaaat tagataattt agatatatat attactggtt
    29461 cgaactctaa aatgttatct aaagatatat taacccaatt tagaggcaga acccatcaaa
    29521 taagattact tccactatct tttaatgaac tatataacca ttttggaaac actccggatt
    29581 tattagataa ttattttatg tatggtggta tgccagaaat atacactaag tttcgtaatg
    29641 aagaagctaa aagagattat cttaaatacc tttttgagga aacttatatc aaagacgttt
    29701 tagaaagaaa caatattcaa aatgatacat ttatattagg cacacttctc gattttatat
    29761 cgtctagcgt tggttcttta attagtcctt ctaaaatttc taatcgattt ttatctgagc
    29821 aaaatataaa aatatctagc gctacagtcg atcgatattt agaatatttt gatgatgcat
    29881 ttgtattatc aaaagctaat cgatacgaca taagtggagc aaggtattat aagagcaata
    29941 acaaatacta ttacactgat gtcgggttaa gaaatgcaag gcttaatttt ccactaaaag
    30001 atcaaggaca catcatggaa aatataatat atatagattt aataaaaaga gggtataaag
    30061 tagatgttgg aatagtacat gataattatc gagtagatgg gagacttaca caaaaacagt
    30121 tggaagttga ttttatggta acaggggcaa atgaacgata ctatatccaa tctgttctaa
    30181 ctttagatga tttaacaaca acagaaagag aaacaagacc actcaataaa ataaaagata
    30241 actttattaa aatagtagtt atcaaaaact acactggttc attttatgat aaggatggaa
    30301 tattttatat taatttaaaa gattttctat taaatgaaaa tttagcggaa ataactaaaa
    30361 gatggtaatc attataatga aacgatagtg ttgttaatgt aattatcata ataaatttgt
    30421 aggaaaaagg cggtctaaaa tgtaggtatc tacacttcta atcattcaga aaatagcgtt
    30481 tagataaaag gactattaca tctagcaatc atctttcact tttttcacta tagtttcaca
    30541 tgaatattta tttaaaatct agtaaagtga tttttattaa gagagaagca tagtgacaat
    30601 ttcagaaaaa atcaaaaaat taagaaaagc gcaaggtctt acccaagctg agcttgctaa
    30661 gggagtaaat gtttcgcgaa cacttataaa taaatatgaa aatggtgccg ctactcctac
    30721 cgatggtaac tttataagtc cttatgctgt agtaagtaaa aatggtttaa agtacacaga
    30781 tttaagtaga actattactg atgctttcgc taatgaagag attttagata tgcaaggtat
    30841 tactgaggct attagtcgct attacttcac aaataacgag aaacttgatg ggattgctgt
    30901 tgctccagaa tatcaagaaa ggtttgaaag gctcgttagt gacgcaattg aatatcacga
    30961 agaatagagg ataaagtaat gaacatttca aatgaagaaa aattaatgta taaagttatg
    31021 aaagctatat acgacagcgg tatcccagta agtttcaaag gctcgttggt gctcaaagca
    31081 tttctacttg agtctggata taccaaagat actcgtcaca ctgtcgatat agacgcaaat
    31141 tggaatggaa aaactacacc tacaatggaa caaattactg aatccttaca aaaagcgctt
    31201 gataaagcaa agattaacct cgatgttact tattttagga caataggtct gctggatttg
    31261 aattaagaga caggtcatct cgagaactac tttttaccat ggacatagat gttaatcgtc
    31321 taatggttaa gacaaagatt tatgaggttt caggactatg ttttaaaggc gttatcccgt
    31381 cgcaaatgat cgcggataaa ttatctgtta tttctataga caaggtcttt cggagaatta
    31441 aagatcttgt ggatttatat tatctttctc atgtttgtga ttttaatagt gaagatgttt
    31501 taagtatgct caaaaataac gggaaagagt taggtaaatt tgatggattt ctaaaccgaa
    31561 caaaagattt aaaacattct tacgaaaagt ttcgttttgc aggtggagtg agtaagccga
    31621 catttgaaga agtatatttg gcagtaaaga cttttattgt agatgtcctc ccacaataac
    31681 aatcgctagt attatgaact aatcaaagga tttaactatt aataaattaa atcaagactt
    31741 tttgtttcag caaaaaaata ttgcacatat tgtaaaggat gcgttaatga cagcaaaggc
    31801 cattacatca ttaaatacaa ttattcattt tttcacaaat ttccattgtt aaaactaaat
    31861 tttaatcgta aatgtatctt tttccacgat aagttcttct gcatattcta attgaacgtt
    31921 aacttcaatc acctctattg gtgtcagtat ttgtgttctt tttttacctt cccattccag
    31981 ggaatcccgg tggcattggt ccgccttttt tatatccctt catttgtttc atcatttgtt
    32041 tcatttgttc atatttctta atcactttat taatctcttg attactcgtg ccactgcctt
    32101 tagcgattct tactttacga ctattccgca aaatagcggg gttacttctt tcttctggcg
    32161 tcattgagtt aataataact tcaaagagct tcatttcgcg ttcggcaagt tcttgttgtt
    32221 caggagtaat tttaggcatg cctgggatta attttaaaat ccccccgagt tttcctaatt
    32281 tttgtacttg tttcatttgt tcgagcatat catttaaatc aaactcgcct tccataatct
    32341 tgcgcgttgt ttttttcact gtcttttcat caagttgttg ctgtgctttt tcaacgagcg
    32401 ttaaaacgtc ccccatccca aggatacggt ccgccatccg gtcaggatag aaaatatcta
    32461 agtcttctag cgtttcacca acaccagcga atttaattgg tacaccagtt aaatgtttaa
    32521 ttgataaagc cgccccacca cgagcgtcgc tatcaagttt agacataata gcgcccgtaa
    32581 gccctaactt ctcattaaag gataaagcag tatttaccgc gtcttgtccg ctcatggcgt
    32641 caacaagtaa taaaatttcc gttggtgtca ctgctgcttt catctctttt aattcattca
    32701 ttaatgtttc atcaattgtt aagcgtcccg cagtgtcaat aattaagaca tcgtatcgat
    32761 cgtcatatgc ttttttcttc gcggctttcg caatttcaac aggactaact ttatcaccta
    32821 aattaacaat atcaacaccg acttgtttcg ctagtgtatc aagttgatca attgccgctg
    32881 gtcgatagac atctgctgca gcaagtaaca ctcttttatt cattttcttt ttaaaatgca
    32941 gcgctaattt accagcggtc gttgttttac cagttccttg aagaccaaca agcataataa
    33001 ttgttggttt atttttttca aaaattagtt cagtatcagt gctccctaaa agttccacta
    33061 attcatcgtt aacaattttt acgaccattt gaccagggtt aagtgtgtca taaactttcg
    33121 tgcctagtgc tttttcttta atattattaa cgaattcttt tacaacttta aaattaacat
    33181 ccgcttcaaa aagggcaatc cgcacttctt ggagcatctc gtccatattt ttttcggtaa
    33241 gacgcgattg accgcggact ttttttaata ttttactaaa tcgttcggtt aatgattcaa
    33301 atgccatcat cttcctcctt acttaagagt tctttaactt cttgtaatgt tgaagtgttt
    33361 tttatttctt cgtgcagtgc ttccacttgt tggcgataag cgtaatgccc cacaatactt
    33421 tctaaatttt ctaagtgttt agtggatact ttaatcgcat cgctcaccgc tgctttagtg
    33481 atgtttaatt cttctgctat ttcttggaca ccaagattat atttgtaata gctttccata
    33541 atctttagtt gtcgttttgt gagtgtttta ccataaatta gtaataactc attaatatac
    33601 atatactctt ttaattcatc catttttatc accaagacca gagtaacgaa attataaaga
    33661 taatcgcaaa attaattaag aatgtacgaa gcacaaaacc gtgaaagaat tgtgagcgcc
    33721 atttatctct tcccatatat ctaaattggc gaatggcaaa gataagacta acaataaaac
    33781 taccaatgat aataattaaa atatccgcga acaaatagtt tgaaggatta agcgaatata
    33841 ccgcaaagcg cactcccata aacacaagaa aagacactac taaactaagt aaggaaataa
    33901 caaccattat tcgtcctccc ctaggcataa gccatataaa tatttttcta aatcaaattc
    33961 atctaagtct tccatttttt cgcctaagcc aataaagcgt actggcacac caatttcatc
    34021 tctaatcgct aaaattattc cgcccttact agtgccatcc atctttgtaa taacaacacc
    34081 agtaagccca gttgcttcgg caaatgcttt agcttgcagc actccgtttt gtccagttgt
    34141 cgcatcaata atcaaaaata cttcatgcgg agcattttct atttggtcac caataacccg
    34201 gcgaatcttg cttaattctg ccattaagtt caccttagtt tgtaagcgac ccgcagtatc
    34261 aataataatt aaatcagcgt taattttctt cccttctgcg actccatcat aagcaacact
    34321 tgcgggatca gcgtttggtt ttcctgtcac aatcgggaca tcaaggcgct ttgcccatac
    34381 ttcaagttgt gcaaccgcac cagcgcggaa ggtatcgcct gcaactaata aaactttttt
    34441 tccttctttt ttatagcgat gcgctagttt agcaattgat gttgttttcc cgctgccgtt
    34501 aacaccaacc acaagtaaca cggttggacc attgtctgca aactgaattt cgttaactat
    34561 gtcttccccc tgctttaagt aaccaataaa catcttatcg actaaaaggt cattaatagc
    34621 ttcagggtca gttaaattat taagcagtgc ttcttctttt gttgcttcaa taatttcata
    34681 agttaaagta acaccaacat ctgcgccaat taagatttca agtaagtgtt cataatattc
    34741 ttccgttact ttttcatagc gttttgctaa gcgttttagt ttactagaaa agttcgcctt
    34801 acttttttct agaccgacaa catatttatc ggtggtttcg tttttcttcc caccaaactt
    34861 cttttttaaa aatgaaatta atcccatctt accgcgccac cttttctaaa gcattctttg
    34921 ctgcattttg ttcggcttct ttactatttg cgccgtagcc acacccaaga ataatcccgt
    34981 catatgtcac tgttgaaata aaggcttctt ctttattatc gtaacccgaa ggttcagtga
    35041 tgtattgtgg taatcccctt ttatcagcct gcatgaattc ttgtagcgta ctaatgtaat
    35101 caaaaatttg attaaagtca tattgctcaa tcgcactgcc taacattctt aaaataaagt
    35161 cttccgttac ttttagccct tgatcaagat acatcgcacc gattaatgcc tcaaaaacgt
    35221 cttcaaaata tttatcgtta ctatataagt cttgcggtgt aattgattta ccagttctaa
    35281 tatagcgttc taattctaat ttgcgtccta aagtagctag cgtttgtgtg cgcacaaagt
    35341 ttttccgcat attcgttaat tcaccttgtt ttttatcagg atgactttta taaatcgcac
    35401 tagcaatcag catatttaaa atagcgtcac ctaaaaactc aagcctttca tagtcatgcg
    35461 cgtctttgtc ttttgtttta taactagaat gcgttaacgc ttcttcaaag agtccctcat
    35521 tacttggcgt aatccctaat ttagtaaaaa gactatttag tttcgacatt ttttaaccct
    35581 tcacgtactt tattcacaat atcagctttg gccattttgt aaccaacaag aatagcgcta
    35641 taaaagccgc gtggatcact attaccatgt gctttaacga caacattatt aacaccaatt
    35701 aaaatcgctc cacctgtatt tttataatca aaggtttctg ccatgttttt gaaacctttt
    35761 cttgcaaaga gatatcctaa tttagaaaat aagtttcgtt taaaagcgcc ttttacactg
    35821 tccattaaga cttttgcggt tccttcaatt gatttaagta acacatttcc agtaaagccg
    35881 tctgctatca caacatcagc ctcgccattt aacgcgtctc gtgcttccat ttgacccata
    35941 aagtaaggta attctttttc tttataaagt ttaaaagctt caagcactaa aggtgaacct
    36001 ttggtgtctt cagttccgtt acttaataaa taagtttttg gtgcttctac tccaaatacc
    36061 gctctagcgt attgttgtcc cattagggca aattgatata agtgactcgc agtattttcg
    36121 ttacttgccc caatatcaag aattgcgact tgtttgcctt ttattttcgt tgggaagggc
    36181 gctaaaatgg cggcccgttc aacaccttca attaatttaa gtttaagcgt tgccgcactt
    36241 aaatatgggc ctgttccgcc tgcactaata agcgcatcag ccttttcttc tattaccgta
    36301 ttaattgctt ttaaaagcga actttctttt gcgcgtaaca cttcaagcgg tgtcgcatca
    36361 attttaacaa catcacgcgc atcaacaatc gttgcgtaat ccgcaactgg cgctaaatct
    36421 tctttgcgac ccacagcaaa aagttcaact tctggataat cttttttaaa ctggataagc
    36481 gctgggattg tgctttcaaa tccgtaatcg ccacccatca tttcaattac aagtttcata
    36541 gtcgttatct ccttattaaa aataaccaaa gttattttac cataatcacc tttttatata
    36601 aactaagccg tttaaagctc tgcactttca actctttcaa ttttttaaat cttgaaagtc
    36661 aaattaaaaa taacctttat aatgagacga caatttcgta ttttgtgact taaaacaatg
    36721 aaaaagtaac tataattcct attgattaaa agtgtatgtt cctgcaaaaa tattagacag
    36781 atatttatat tagtcttttt ctaaagcatc attaattact tttaaaagcg gaaaataatg
    36841 cggatttgtc gggttattaa taacttccaa cgcatcttct ttaatgatag taattagttt
    36901 taaatcctta ataacattaa gatacataaa gctaggcatc ccgctttgct tctctcccgt
    36961 taattcacca ggcccgcgga gtgctaaatc aagttctgct attttgaagc cgttatcttc
    37021 tcttgcaaac tcttcaagtt tataattatc ttcttcctca ctcactaaac aaagtgttgc
    37081 ccttttcccg tcgcggccaa ttctgcctcg aagttgatgc aatgaactaa gtccaaaact
    37141 atgtggttcg taaattatca taagtcccgc actcttcaca tcaatcccga cttcaacgac
    37201 tgtcgttgac actaagattg gtgttacacc acttttaaag tttaagagcg ccgcgtcttt
    37261 ttcttcgctt gcaagctcgc cgtgaagcat acccactaaa tgtgggtagc gcgctgaaaa
    37321 acgctcgtaa agcgctaaaa catttgtttt atcaccgaaa ctaatcttcg gtgcgactat
    37381 ataaacttgt ctattttccg agagtgtttt gtcaataagc ggtatcacat ttttagacgc
    37441 acttgtcgta ataatggtat tcacttgtcg cccagtatgc ggaaaacttt caattgttga
    37501 aatatccata tcgccataaa ttacatttgc aagtgtgcgc ggtatcggtg tcgcgctaag
    37561 taagagtaaa tcagcctttt cgcctttgtt taatagtgta ttccgctggt taacgccaaa
    37621 tttatgttgt tcatcaatga ccacgagttc aagtttagga taaaagacat cttcactaaa
    37681 taaagcgtgg gtaccaacga caatatcgac taacccatgt tggatatctt cttttaattt
    37741 ggttttaaca cttggcgtta aagccccaat tagtaattcg attcgtaaat caaaatcctt
    37801 aaagaatgaa aggagcgttt cataatgctg tttcgctaaa ctttccgttg gcgcaagtaa
    37861 gacaccttgg gctccacgca agtaattaat atagagtgcg acaaaactca caactgtttt
    37921 accgctccca acatcaccct gaagcaaacg atacatcagt gtttctttat tcatatcgta
    37981 accaatttct cgcacggcag ttagttgatc tttcgttaac ttaaatggta gtcttttaac
    38041 aaattcattt gcgtctttta aattaacaat tgttttcttt gaattagtta attcgctatt
    38101 tgccctttta ataagtaaaa gcttaataaa atattctaat ccttcttcgt atttaagtgc
    38161 tttaagtcct tcaaagacat catttaagtc acgtggatta tgcacacgtt ttaaggcatc
    38221 atttagcgaa attaaaccgt ttttgtcggc taaagggcgc ggaacacgcg aagttacact
    38281 ttctatttta agtgcttttt gcacgattct tataaactca tgattgccta cttcacttgg
    38341 taaactatag agtggactca gctgttcttc ttcgcttagc acccgcggaa agagtttaat
    38401 aactgttagt tctaaaagtc gcaaattaat tgtgccgtat accgtgatta agtcacctat
    38461 tttgacggcg tttaatatat agcgttggtt gtaggcaaca attttataag ttgcgccact
    38521 ttcactaaca gcggtgaaac gaataatcgc cactctttgc ggtttaatta aaagtggtgc
    38581 actcgttact tttaatttaa gtgtcacttt ttcattcgtg ttcatcttaa attcatcagt
    38641 gtacgacata tctaaataac gacgcggtaa atgaaaaatg acatccctaa atgtgtggat
    38701 gtcatatttt gctaatgcat taataattcg tgatgaatta cttattttcg ccatcgctac
    38761 ctctactcga gaccgacgat aaacgaatat acaggttggc cgccatttac taattcaaag
    38821 tcaactgccg aatatttttc ttcaagttcc gcaagtaatt cttctgcggc gctctcttca
    38881 acatcttcac cgtaaattat cgtcacaatt gctgttaatt catcaatcat gccagcaagg
    38941 agttcgcgaa gggcatcaaa tttatcaggt aaggaacaga gaatcttttt accttgtaaa
    39001 cccatataat aatctttttt aacttcaaaa ccatcaattg atgtatcttt aattgcaaag
    39061 gtaatttgtc cagtttcaac agcgtcaagt gcttcagtca ttatttcaat gttatgttca
    39121 gtatcaactt cagggttaaa gttcatcgct gcgactaagc cttgcgggat tgttttactt
    39181 ggaataacgt gaacattaac aacaccttct tcattagtga cttcagcagc ttgggttgcc
    39241 gctaaaatga tgttaccgtt attaggtaag atgaaaacag tcttagcatg cacctttttg
    39301 attgctgcta aaaagtcttc ggttgacgga ttcattgttt gtccgccctt aactacataa
    39361 tctacacgat attctttaaa taaatcactt aatccttcac cactagcgac agaaattaaa
    39421 gcgtagtctt tcatttccat gcttgcacta gtcacttgcg gattagtgtt aaaataatct
    39481 ttgccgtgat cacttaagtg cgtatgttgt tccgtcatat tttcaatttt aagcgtgaca
    39541 aattccccaa attgttgcgc ataagttaaa gttgtccctg gtcttaaagt atgaatgtgc
    39601 acttttaaga tatcttcgtc gcgtaccatc acaattgagt taccttgtga atttaagacg
    39661 cttgcaaaac gctcttcatt aaatgttttc tttacttctt caccagcgcc aattcgtaag
    39721 ataaattcag tacagtaacc gtattcctct tcatcagtga tccgcgctcc cgccatttca
    39781 tattgcggta caacttccgt tgcggttgca ctttcttttt gaataatacc ggtagtaatg
    39841 gccgcaagca ttccttctaa aatggccaca accccagcgc caccactatc gacgacgcca
    39901 acttctttta aaactggtaa taaatcaggg gtacgctcta aacttgcttt agcttccgca
    39961 accattactt caagcgcttt aacaattgac caatcaggct tagctttttc gtttaacact
    40021 tcagccgctt cacgaataac ggtgagcatt gtgccttcaa caggacgcat aaccgctttg
    40081 taagcgacat ctttaccgac taacattgct tcaccaaatt ggacagcgtt cattgtttct
    40141 ttaccttcaa gcccattagc gaacccgcgg aaaatttggc tagtaatcac accactatta
    40201 ccgcgtgccc ccattaagag ccctttacta aaggcgcggg cgatacgata gacattcgta
    40261 tcatttaagt ttgatatttc ttttgcccca cttgagacag ttaaattcat attaagtcct
    40321 gtgtcaccat caggcacagg gaaaacgttt aaagcatcaa tcgccgggta attgttatat
    40381 aagtggtttg ccccggagat gaacatttcc ttcagtgtaa ttccattaat ttctttcatt
    40441 ccacaaactc ctctttagcg ttcctcaacg ccgacaatat agatattaat cgctttaaat
    40501 ttgatgtcaa aagtcttctc taatgagtat ttgacttggt cttgtaccga tgtaataatt
    40561 tctgttaatt ttaatccgta cattacattt aaatataagg aaacatgcca gctgccactt
    40621 acttttttaa caacgacacc aggctctgga tcaacattaa aaatctcgcg aagccatgac
    40681 ttcggcggtt gatttgcaat cccaattacg ccataacacg acgtcgccgc gtcaacagct
    40741 gcgctcgcga ttgcttcttc actaatagta attcctccaa acggagtttt gttttttgtt
    40801 tccataatat ttgtttacga ggctttcgaa aaaggacatg aggttcgcct cgtagtgata
    40861 ttatactaca acatgaacgt tcactcaaca aaataaccta ttattaagta acattttttt
    40921 atat
//
LOCUS       DAOSLP010000006        37802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_1252_length_37802_cov_10.460699, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000006 DAOSLP010000000
VERSION     DAOSLP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 37802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37802
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1252_length_37802_cov_10.460699"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..456
                     /gene="rplL"
                     /locus_tag="PK460_01225"
     CDS             82..456
                     /gene="rplL"
                     /locus_tag="PK460_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOE53758.1"
                     /translation="MAKLTVTDIIAALKEMTVLELNDLVKAVEEEFGVTAAVATAGPA
                     PEDDGPVDKGPSEVTLVLKHWGQSKVAVIKAVQAITGLGLMDAKKLVDGAPVNIKEKI
                     SPVEAEEHKKALVEAGADVEIK"
     gene            527..1126
                     /locus_tag="PK460_01230"
     CDS             527..1126
                     /locus_tag="PK460_01230"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOE53759.1"
                     /translation="MNHYFANNNELEHDIRTLELSLQGTNLTLITDRGVFSNKEIDEG
                     SIVLLKSIIKSIDVKGEVLDVGCGYGTLGLYLAARYPEIKVTMFDINERAVSLTSENA
                     KRNNLTNTHVYVDSDYQALPKHTFNLIVINPPIHAGKKAYYPLLSAARDYLTDGGILM
                     FVIRKSHGAKSAAKYLSDYYSNITLIQKDAGFYVYHAQK"
     gene            1154..4777
                     /locus_tag="PK460_01235"
     CDS             1154..4777
                     /locus_tag="PK460_01235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015373724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOE53760.1"
                     /translation="MKTINDKTKRLHNDRVDFSKISGNFEVPNLVGIQIDSFEWFKNE
                     GIAEVFNEIFPVESYSKDIEIGFEGFRFDTPKYTYLECKDRGFTYSAPLRVTLSLGIR
                     KTGEVVKSEIFMGDIPLMTHTGTFVINGAERVIVSQIVRSPGAYMSKELDTKSGKFIY
                     NADLNPTRGTWLNFENDAKGLLSVRIDKQRKMNATLLLKALGITDYHETIELFDVFDA
                     KNRAKNNKRKSEEEMTSLELTLRKDESFTTSLEALYEIFRRLKPGEPMSDEGVKNHLI
                     QKFFDPKRYDLGKAGRFKFAKKLGIYERLEGVVLAEDLVSVDGEILFEKEHQMKKEDV
                     DKLRALKFFEKGAHTYTIPFNPDLDDYNTVNIVKAYVNDEKTQAINIVGTDLNIDEKR
                     VTTPDIVAVFSYFLNLMHGIGRVDDIDHLSNRRVRPVGELIQNQFRVGLSKMVKAMKD
                     SSSIADTETITVQKLVNTRAVASAIRDFFATSQLSQFMDQVNPLAELTNKRRISALGP
                     GGLTRDRASFDVRDVHYSHYGRICPVETPEGQNIGLINNLACYAKVNEYGFIETPYRK
                     VLKPYGQITNETIYLDADEEKEFVIAEANVEVDENGVIQTQEIIARYQGENVLVSPDK
                     VDYIDVSPKQIVSIAAASIPFLENDDAMRALMGANMQRQALPLMVPESPYVGTGLETK
                     IAHDSGSAITAHKDAVVTYVDGANISVKFDDDTTFTYKLQKFARSNQGTCFNQVPIVK
                     VGDKVKKGQVLADGPSMVNGELSLGRNVTIAFMTWDGYNFEDAVIMSDRLVKEDVYTS
                     IHIEEYKIEARKIKTVSERITRDIPNVSEAAKAFLDENGIIIPGAEVKEGDILVGRVT
                     PKGQREQSPEDRLLQTIFNEKVREVKDTSLRVPHGGGGTVIDVKVFTREAGDELGPDV
                     EISVSVFIGQKRKISEGDKMSGRHGNKGVISRILPEADMPFMEDGTPIDIMLNPLGVP
                     SRMNIGQVLEIHLGMACKKLGVKVASPVFDGMTNEEIFELMEEAKMHKDGKEILYDGR
                     TGDRFDERISVGVMYLIKLVHMVDDKLHARATGPYSLVTQQPLGGKAQNGGQRFGEME
                     VWALEAYGAANILQEILTIKSDDRVGRKRTYEAIVKGYDLPTPGIPEAFKVLVRELQG
                     LAINVELRMKDGEILDLDKLAKEQDADIRRSRRSTAVREEQEVFDPEETAVVYKNEER
                     GDE"
     gene            4780..8646
                     /gene="rpoC"
                     /locus_tag="PK460_01240"
     CDS             4780..8646
                     /gene="rpoC"
                     /locus_tag="PK460_01240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOE53761.1"
                     /translation="MARKQPFNINDLASVQIGLASPDMIREWSNGEILKPETVNYRSQ
                     KPELDGLFCERIFGPSKDYECHCGKYRRIRHRGTVCEKCGVEVISKYVRRERMGHIEL
                     ASPCTHIWYLKGTPARIATLLDIPAKQLEEIVYFISHIVTDPGTSSVLSYKQFIDETT
                     GRQVFAEAIKEIQENIEEGSFEHAKGSELLERLEVTQTFDFFATSRYISKHTGAKFGE
                     GAEVIKQLLKEVDLQKEFDEVNAALKTESKTKEKKLLRRLEVINAFLKSGNRPEWMVL
                     DVIPVIPPDLRPMLQLDGGRYATSDINDLYRRVISRNNRYKALVQMNAPKVILMNGQR
                     MLQEAVDSLIDNGRRGKPYTGANKRPLKSLSSSLKGKQGRFRQNLLGKRVDYSGRSVI
                     AVGPELKMYECGIPREMAIQLFRPFIAAELIKRDSRATNHTQADKIIDRHDEIVFDII
                     EEIIADHPVLLNRAPTLHRLGIQAFQPVLVSGRAIRLHPLVCAGFNADFDGDQMAVHV
                     PLSEAAQEEALNLMLASKNILGPKDGKPIAAPSQDMVIGNYFLTVEESREDFVRRAEE
                     ARALGDEFGAQKFMQYAESEGKVFGSIDELMVAYQTNSVHLHNRIALPGRAFNKTLFT
                     PEMNKSYLVTSVGKVIFNTIFPNDFPYLNEPNKGSLQMDKIEYFVPAGTNIREHIASL
                     PLRKPINQEFLGLIINEVFTRYGTVKTSAILDKIKDQGFHYSTKAGLTVSLHDITPIE
                     GKEELVEAGEVEVRKINKAAARGFYSEKERYNAVINVWKGVKDDVEAKLKEQFPLDKR
                     NPLYIMFDSGARGKTTNFLQLKGLRGLMASPRGGEIEIPIKSNFREGISVSEFFIASH
                     GSRKGGADTAIKTADSGYLTRRLVDVAQNVIVTEEDCGSDHGFVVTEIRIRENDTPAV
                     PLSDRIAGRYTVHDIIHPTTGEVIIEANTLINKDAALYIEKAGVKEVEIRSLFGCEAP
                     SGICAHCYGTNLATGERVKVGEAVGIMAAQSIGEPGTQLTMRTFHSGGVAQVEDVTQG
                     LPRVQELFEARNPKGKAIITEIAGEVVSITQEEDRRYTVTVKNDLEEKSYLTEYGQQL
                     RVKEGDTLKQGDRITEGAVHPRELLAVSTITAVQNYLLNEVQKVYRISASVRISDKHL
                     EIIINQMLNRVYIIDGGDTSLLPGQRITTHQFTAANKEALINGLHPAVATPLILGIKA
                     AALASDSFLSAASFQETTRILTDATVKGKLDPLHGLKENVITGRLIPAGRGILTKEEQ
                     EALLEDFSVADTLDKIKASYDNSSNS"
     gene            8787..10382
                     /locus_tag="PK460_01245"
     CDS             8787..10382
                     /locus_tag="PK460_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53762.1"
                     /translation="MKKRLLLLPLLAMLLGGCNGGSSATPSISTPESIPSSEEPSVES
                     SAESVSVSIDTTSYEIATTTNYSNRELDHSIDMTALDSLGTQKMLVVPVVFSDTAHRA
                     TEEVHTMINKAFFGASEETGWESVASYYHKTSFGKLTLTGEVLPYFYSQWSTHAFGAL
                     EVSPTQEYGEGYYWDQTHHLIEEIYNTLDAELFLEYDQDGDGHVDAFWMVYIAEIKAL
                     GGEDDPFWAYKFYWNRYANTEKPTPNTYAWASYKFADEGVGYNFNIPDAHTYIHETGH
                     MFGLPDYYDYDSKTAPAGALDMMDHNVADHNMYSKYRLNWSKPFVVTGNADIHLRPAE
                     STGDFILIKDGWNGHVYDEYILIEYYTPTGLNEKDSQGRGYETKSSNVSTKSFDINGV
                     RMWHVDSRLMTYVFDPESLELTNAFWTDEIVVGDPFSYTIIGPSNTTSRSSTPEGDSK
                     VNKLLHLMDATGTTSRVGNWLRTPTTAGSTALFQTGDVVAADGWKKYIQTSATFNDGT
                     AVGYSVEIGEMDDNGVQIIIRKA"
     gene            10527..11966
                     /locus_tag="PK460_01250"
     CDS             10527..11966
                     /locus_tag="PK460_01250"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOE53763.1"
                     /translation="MTFFPYTHHISDTDTYTRIETSDYYKVGAFARSYQDVLKSNLNG
                     NSRVVPLKSSGEQKILVLPVNFPDYDLNELDGNNGKTAHIHIQNAFFGENHTTRFYSV
                     AGYYEASSYGKLKLSGDVAPWYTLPAEYSVSAIKSRVRSNSDKLNETAEILNLALKEY
                     AKSNDLSAFDLDENGVLDSAYVIYSYPFEPKDVNNIFWAFAANMNKKDPELTHQANAY
                     SWSSYYYLDVARNKKPDPHTYIHEVGHLFGLPDYYNTNYDDPYSPLGGFDMMDYTLGD
                     HTGLSKMLLDWARPYALKRSTTIKLRPFSETGDLLVLKNNWNEKANDEYILLEYYTPT
                     VLNELDSTLNASFKLPKTNGVKVYHVDARTVYEIKNGSIVKYPYVNDYEGEFNGTELL
                     AHSNSTGSYNNAPIKTNKLYKLLEPKESTNISESFNQANQHSLFQKGDEFGYEYFKNF
                     AFNDKTPLNFRFKIVQLTNAYATIEIEVY"
     gene            11968..13590
                     /locus_tag="PK460_01255"
     CDS             11968..13590
                     /locus_tag="PK460_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53764.1"
                     /translation="MRKSTFIRRALILVVPALLLTSCVTFGDILKSSEFGATSSSANE
                     VTSEVTGSENPYPLNGTYGVNDYEHPITANPDTLNNYYKVEPLNNNSRLINNYAISNT
                     MGLAHLDSVSNQKMTVVPVKFTDTPASYGGEATIQGAINRAFFGESSDTGWESVASYY
                     YKSSYGRLSLTGEVMPLFDMGYSTTELLAKTVDGYQYWDKSHYVLEQVYNTYLSSTLK
                     EYDQNNDGFVDSVFFVYLAPVDYESDDTLFWAFQYFWNRFADKNKPVFNAYGWASYEF
                     MVEAPGYTYLKPAAHTYIHESGHILSFDDYYDYDGTIAPAGAIDMMDHNIIDHNMFSK
                     FSANWSTPYVPVGNSDIRLRPAESSGDFIIINDAWNGHAYDEYILIEYYTPTGLNARD
                     SLSVSGYNGVKGYTIPGVRIWHVDARLVILSNNGNTKKWSDTIPPHLDGSGGSWMDFA
                     TSNTPSYNYAHNSLHKLHLLDGRGRAQTWANNLVKADDSALFQTGTVIASNNWQKYLL
                     KRATFNDGSVIGYSIEIGEMNSEGVTIKIRKA"
     gene            13645..14598
                     /locus_tag="PK460_01260"
     CDS             13645..14598
                     /locus_tag="PK460_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE53765.1"
                     /translation="MATLKDIAKATGFSVITVSRVINSPHLVKEKTRKIIEAEIARQN
                     FKPNVLARALVQNRTNIIYVYIPADIDAINPFFLHVVSGIGEVLGEKGFSLLLKRSWY
                     EGEDCDGCIFMGLSAEDEAKVFELSKTKPVCVFGYVEGVSSVDVDNYQGLYLMTSHVL
                     SKGYRTLTYLGIDDYRRFILQRKKGFSDAVMSQKDKITVKYLAAKNNENDAYKVVGQS
                     LKQGDTPEVIVCATDDMAIGAIRAIKEKGLEVPRDIAVTGFDGLGTQEVAHPRLTTIH
                     QPIYEAGKALAHRIIALIENKNELPVNHAFIETTIIHRESI"
     gene            14685..15203
                     /locus_tag="PK460_01265"
     CDS             14685..15203
                     /locus_tag="PK460_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE53766.1"
                     /translation="MKPKKITTILFSLFLALSLSVVTGCKDGSENGGSDDPINFWAYQ
                     PSKQTDQTAYKNLIADFTAETGIKVKLLMVVKDSYNKSLNAAISSRSKPDLAYFDQPR
                     ISDYAHDDTIAKLDEYIANSAVIDNTKFFASGLATTVYKGSQYGVPLNMTTSVLFYNK
                     DIVTTPPTSWQE"
     gene            15375..16052
                     /locus_tag="PK460_01270"
     CDS             15375..16052
                     /locus_tag="PK460_01270"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HOE53767.1"
                     /translation="MFYDPNKVLSSGDVYLYTWDGDGRKITGNWPGTAMFNHGFGYYS
                     WFFHAAYLTENVIFHDNAGNQTGDIVWEIGKNVYHQETGTWTFVSEVHHKATVYASKI
                     LEKTATCDATGAANNLNAPEWNETLANQYNDSLDPTTKAYLKTAVADSNGDLLAQAMA
                     RYDYIVKKYGTAMFSNFLDRTIPASGSPLVSNEGTNNALVMGAFIVMALVALAGFKLI
                     SKKHRYH"
     gene            16174..16410
                     /locus_tag="PK460_01275"
     CDS             16174..16410
                     /locus_tag="PK460_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOE53768.1"
                     /translation="MIIKTEQIITMTDANQNFSKASKTAEKYGSAVIFKNNKPKYILA
                     DIEQYIELTEDEKIEIVAKRILSKHINAFKNLAK"
     gene            16481..16600
                     /locus_tag="PK460_01280"
                     /pseudo
     CDS             16481..16600
                     /locus_tag="PK460_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            16613..16786
                     /locus_tag="PK460_01285"
     CDS             16613..16786
                     /locus_tag="PK460_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53769.1"
                     /translation="MDGNKRIGLYVMLSFLQINGIIISFTDDEIEQIAFGIASGAINY
                     VDLKRILEQKIDN"
     gene            17042..17464
                     /gene="rpsL"
                     /locus_tag="PK460_01290"
     CDS             17042..17464
                     /gene="rpsL"
                     /locus_tag="PK460_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOE53770.1"
                     /translation="MPTINQLVRHGRQKAVYKSKSPALTKRWNSLKRREVNQYSSHKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNGYEVTAYIGGEGHNLQEHSTVLIRGGRVKD
                     LPGVRYHIVRGTLDTAGVEKRRQGRSQYGTKRPHTAEH"
     gene            17512..17982
                     /gene="rpsG"
                     /locus_tag="PK460_01295"
     CDS             17512..17982
                     /gene="rpsG"
                     /locus_tag="PK460_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOE53771.1"
                     /translation="MPRKGSVAKRDVLPDPIYHSKLVTRLINKLMYDGKRGTAQTILY
                     NAFERIEAKTKQPAMDVFATAISNIMPEVELKVRRVGGQNYQVPTDVSPERKTTLGLR
                     WLVNYSRLRNERTMEERLAAEIIDAANGTGASVKRRDDTHKMAEANKAYAHYRY"
     gene            17991..20072
                     /gene="fusA"
                     /locus_tag="PK460_01300"
     CDS             17991..20072
                     /gene="fusA"
                     /locus_tag="PK460_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004028937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE53772.1"
                     /translation="MADLTKFDLQHTRNIGIMAHIDAGKTTTTERILYYTGRLHKVGE
                     THEGASAMDWMAQEKERGITITSAATTAFWKENRINIIDTPGHVDFTVEVERSLRVLD
                     GAVTVLDGKSGVEPQTETVWRQGTKYNVPRIVFVNKMDATGADFNMAVSTLHSRLAAN
                     AEPVQIPIGVESNFRGIIDIIEEKTYIYDDNIANVSIEEVPEEYLEEMHIAKQNLIEK
                     LAAFDDDFMIQVLEGETPSVSEIKRVIRKAVLTGEFFPVFCGSAYKNKGIQPLLDGVV
                     DYLPSPLDVPPAKAELLDGTEIITPSDADAPLAALAFKIATDPFVGRLAYVRVYSGTI
                     KAGSYVYNSIKGVRERISRLVLMHSNHREEVSELKAGDIGAVVGFKNTTTGDTICDEK
                     NKLILERMEFPEPVIEQAIEPKTRADQDKMTNGLAKLMEEDPTFKVTTNPETGQTIIA
                     GVGELHLDVLVDRLRREFGVNVNVGAPMVGYRETISQEAECEGKYIKQSGGRGQYGHV
                     WIRFEPNPGKGFEFVDAIVGGVVPREYIKPTEEGLKDALVRGLVAGYQVIDIKATLYD
                     GSYHDVDSSEAAYKIAASMALKEAGRLAEPVLLEPIMKVEITVPEQYYGDVLSDLARR
                     RGSMKDESQRGNAKVIDAEVPLSEMFGYVTDLRSFTQGRGTYTMTFDHYGECPRYIAD
                     EIVKKSGTSAR"
     gene            20181..21365
                     /gene="tuf"
                     /locus_tag="PK460_01305"
     CDS             20181..21365
                     /gene="tuf"
                     /locus_tag="PK460_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009007562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOE53773.1"
                     /translation="MAKERFDRSKVHVNVGTIGHVDHGKTTLTAAITKVLASQGGAKL
                     MAYDQIDRAPEEKERGITINTSHIEYQTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGVMPQTREHILLARQVGVPYVVVFLNKVDLVDDPELVDIVEMEVRELLNE
                     YGFPGDDTPIVRGSARDALDGKADAEKAIIELMEAVDSYIPDPERLTDRPFLLPIEDV
                     MTISGRGTVVTGRVERGMVKLNEPVEIVGIRPTKSTVVTGLEMFRKLLDSAQAGDNVG
                     VLLRGVARDEVERGQVLAKPGSVKPHTKFTSTVYVLTKEEGGRHTAFLSNYRPQFYFR
                     TTDITGVVTLPADVQMVMPGDTVTLTVELIHPVAIENGTKFSIREGGRTVGAGTVTEI
                     LK"
     gene            21477..22589
                     /locus_tag="PK460_01310"
     CDS             21477..22589
                     /locus_tag="PK460_01310"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOE53774.1"
                     /translation="MKKEKKITQPNLDTVPGGALKRTGAVLIDLVIMYLLMNVLWFAI
                     HPIYNKVYDIDNVVTAHERDKKRSNLVVLNEPILDDESNIDLVKVESNQLPETKRGEG
                     VFKFYTVFLRSEQEAKLAAGEIDNTPENNAMFTFDWYLENVIKPDTEESIYLRIPTDE
                     HLGAEGAVSSEETAEPEYDTFRPAGLDFKADLTAEDINKFNNRIYNQAVNAFNLRPNM
                     TIINKVNIQENIILMVLGSTVVFLVFPLFFKHGQTLGKKALKLGVTTRHGYTVTVPWL
                     LLRYFAFLFINLLSNLLIPIILPFISLTIMTFSKERRSAHDFIANTKVIDLQNSTVYK
                     SEAEFLEAQKRYNPRLEAEDFNEEVFSERFDEESGA"
     gene            22589..23593
                     /locus_tag="PK460_01315"
     CDS             22589..23593
                     /locus_tag="PK460_01315"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOE53775.1"
                     /translation="MNHYVVKQTAGKRFLAGFLDTIFVIILALFLYIPARTIVDNNGL
                     IEVFVKITQIQLKSGLYDVEGIDNAVPYIINDVEKFPKALYTFYVDENLETGEIERGY
                     ADILVEGHEYNTAEDYYVVILGKGREDSLFTFDAINSEAPWEVPFKEGKQEAVKQFYT
                     DEIEKAHQHLDYHEVVVPLIIKAERLIFYAMAGSYLTSALILIVIIPNFRKDKVTFGK
                     VITGTIVLNRLGYKMSVLQANMRNFAIFIFSFVFFFVPFHLISFAMTLFSKKQLSMYD
                     QLVATLVADKKATLVFKDVNEETKYRKELAQRLIAVDKRKAESREERLRDKEKLNKID
                     "
     gene            23687..24466
                     /locus_tag="PK460_01320"
                     /pseudo
     CDS             23687..24466
                     /locus_tag="PK460_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            25140..26243
                     /locus_tag="PK460_01325"
     CDS             25140..26243
                     /locus_tag="PK460_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE53776.1"
                     /translation="MSTKAGVLHLEKGPMQTKKTIAMPIWLKAIIALLPALFFLLVFT
                     IFPIFNTAFIAFVKDFYFQSGTGSFALASYFRQLDMHNNPDWWREYFAIIEPDVPFLQ
                     PVKPIIGFANFEYVLKDPLFSNALINTAILTIISVPLTIMISLLLAVSLNSIKPLRAT
                     YQTIFFLPYVTNGIALGMVFNTIFSHEVGGIMNQFIQFFGGKPVSWIRPLWSPEGTIP
                     LSKFSLGFAITVQSIWSGLAFKILVFMAGLASIDKQYYDAARIDGSSRSTIFRRITVP
                     LLSPQILYITITSFIGAFKAYQSVISIVGGGPIHFGGENRTMWITVVGYIYMNRNVEK
                     NIPLAAAGSVVLLIVILFITLIQMQVSKKRVHY"
     gene            26259..27317
                     /locus_tag="PK460_01330"
     CDS             26259..27317
                     /locus_tag="PK460_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE53777.1"
                     /translation="MTDKDKKVTEAVELTAEEEVKLSHIDDERFQPSYEIAKEYKTRA
                     IVVKIITYTFLTLFAFFMLLPFWFMISVSVKTPLEYQREVSTGLRLFTANPSFVNFRI
                     VLGMDLKDPLKEMNEALLHIVETSPERSFIPYFLNTIIVAVVSTVFTIVTTVLASFAF
                     ARLEFKGKNFLFAILLATMMIPGEMMLITNIQTAKWLGWENTYRALIFVHGVSVFYIF
                     YLRQTFQQIPNELFLAAKVDGYGEFSYLWRVMIPIGMPTIVTIIILNVMGSWNAFIWP
                     TLIAQGYNPTIQKLFKYNHTMRLVSNGLMSTFTGQFASYDTVKIAASMIVTAPLLLVF
                     VFFRKYIMSGVSRSGIKG"
     gene            27362..28957
                     /locus_tag="PK460_01335"
     CDS             27362..28957
                     /locus_tag="PK460_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE53778.1"
                     /translation="MKFRKLIFTAVSVLALTTLAGCLRPPMPPTSETPTTSSGGGGPT
                     EKIEVKFWTGFGASVTGALNPLFARFEQENPDIKVLYESKSGYPNLQQAINGSISTNT
                     YPHIANGYPDHLVGYINANILVNLGNPTNYINHPDYGVDINEYNSDYMAEINGLSDAA
                     TFGLPFNKSTEVMIANQSFFDAAKKRDPSIFIPKTWQDLAEVGPKLKAVAKDNGWFGK
                     LVLKKDGTAVAKPAEFDAALKEQVAVDMTLVNENQFIPFSWDSASNFFITIIRQWGAM
                     YTEKGNTIQNGYIRFQEEPHRTKTLAALSYFKELYEDGLIGLPDNFGEPLYSSTPFKE
                     GKLVLTISSSAGVGENLPGGTTEYPFELSINPIPYNGDLAAQKSVIAQGTNLALFTVG
                     QGRDPKAQAERLAAWRLLRFLTYEANYEFGKATSYFPVLDASKIDRDSQRYQDYLMYS
                     EFLNQTDGTPREKVIRSTAKLQANVYMDPEAGWNKFVDPGFLGSSEIRNTIESVMAEL
                     FKGKTPEQVLNDTVTKLARYVQK"
     gene            28972..30273
                     /locus_tag="PK460_01340"
     CDS             28972..30273
                     /locus_tag="PK460_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53779.1"
                     /translation="MKKTFVKIGLLLAVFGLLVGCDNSSSEVVPPSSASEEVVSSSEE
                     EVKLTEQEYQIAVNYIARSVPLVLERGVKVPTNVERAEAENPLPTPELKYGDNLLLIT
                     AGELKYEDEINGVYLYPEYTVTYSFLEQEPYAGFTFEYDDIGRYFAVPEYPRYEAEYD
                     NGGNPIHTVPPAKAARLYVTIHIEERFTTFNMDMILEPQAIIHDYNLADVRSVPTGSV
                     VRVYGYFNGYFSDWNNGVLCDGDVALGLFKLQEFNHMELGKMYAVVGQFTVYNGLPQL
                     QWIKTVSEVEPADHPTVKEPKAVVVSADDFADQLDIGNELSGPLMHKVGTTVVLNFPL
                     RYKAMLDRDGNAVEPGALELGKHADVVLTGKDSDNFEFEVKLSLNYHMGTDNQAAFRD
                     FFVTNADADIYFTGPLGAYNAINLAPFAFEGTLSLTPPVVS"
     gene            30362..30437
                     /locus_tag="PK460_01345"
     tRNA            30362..30437
                     /locus_tag="PK460_01345"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30395..30397,aa:Lys,seq:ctt)
     gene            30545..31954
                     /gene="trpB"
                     /locus_tag="PK460_01350"
     CDS             30545..31954
                     /gene="trpB"
                     /locus_tag="PK460_01350"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOE53780.1"
                     /translation="MKYLTVKELSEKWKMSERRIRALIKEKRIEGVKYENKYLIPENA
                     KKPLDRRIKGQRALEDTSLNRQDFDFKNYRKHYPTSDGYFGRFGGSFLPKHLQKAIDE
                     VYDGYLTIAKSAKFIAELREIRKNYQGRPTPVYHCLNLSNHLGKVQIYLKREDLNHGG
                     SHKLNNAMGQALLAKYLGKKKLIAETGAGSHGAAVAMAAAYFDLECDIYMGAVDTQKQ
                     RPNVDRIKVLGANVIAVKEGTQTLKEAVDAAFNAYAKEYKNAFYVLGSAVGPHPYPLM
                     VRDFQEIIGREAKEQFVAMTGELPDVVAACVGGGSNAIGMFQAFLEEKVNIVGVEALG
                     QGPTIGQNSAAVSLGKEMILHGFNSLVLQNEDGSVGDAYTIASGLDYPGVGPEHAFLH
                     EIGRVKYEAITDEEAAEAFFLLSRLEGIIPAIESSHAVAYAIKYARSVSAGSILVCLS
                     GRGDKDMEFMTREYPNMYK"
     gene            complement(32030..32479)
                     /locus_tag="PK460_01355"
     CDS             complement(32030..32479)
                     /locus_tag="PK460_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53781.1"
                     /translation="MILYIPVDFDRMVGITEKLKTPEHSKVTLTGKHNGVDFEQKYTL
                     TDGVWVGDEEYEDPHGYTIKKSVSDHVMTIKSFTVEQQQKYKYFTNSRKFKIEFRDYT
                     SDKDYSYGTFIYNRKGTLMEIHFDSRFVNEEYSINVSDNLLIYYDFS"
     gene            32531..32845
                     /locus_tag="PK460_01360"
     CDS             32531..32845
                     /locus_tag="PK460_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE53782.1"
                     /translation="MIVYMNEHFRDPNLTIAKIAESSGYSLSHFSKAFKLYMGHSPIS
                     HLNRLRLSEARYLLHTTHLPFKEIALQCGFKNISTFTESFKQSIGILPKDYRKKYQTP
                     NS"
     gene            33013..36540
                     /gene="nifJ"
                     /locus_tag="PK460_01365"
     CDS             33013..36540
                     /gene="nifJ"
                     /locus_tag="PK460_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOE53783.1"
                     /translation="MKKKFVSLDGNAAAAEVSYLYTDIAAIYPITPSSPMAELVDLFA
                     SQGKLNYFGSKVKVTELQSEAGAAGAVHGALQAGTLATTYTASQGLLLMVPNIYKWVG
                     ELLPAVLHVSARSIAARSLSIFGDHQDIYAVRQTGVTMIASHSVQEVADLAHAAHLTA
                     IGSSAPVLHFFDGFRTSHEIQKVEVIDENEWKKLLPEDKVAEFRARALNPHANPVTRG
                     GAENDDIYFQGKEAQNTHYARVLDVACDYFAKVSKVTGRHYAPFVYLGAKDATKVIVA
                     MGSVTETIREVVADLNKKGEKVGLVKVHLYRPFSAKHLVDVLPETVKKIAVLDRTKEV
                     GALGEPLYLDVLTALKQQGRKVDVIVGGRYGISSRDTQPKHIKGVFDFLDNKPWHSFT
                     VGINDDVTKLSVPFDHDYHIDNGHTECLFYGLGSDGTVSANKSSVAIIGEHTDKYAQA
                     YFAYDSRKAGGPTRSHLRFGTNTIHSTYYVENASFISASQDSYLYKFDMIRYLKAGGT
                     FLLNTSLKGEELINALPNRVKYGLAMKNAKMYVIDANKIASEIGMGRHTNTILQSSFF
                     YLNQDIMPYEKALRLMKKYAEAAYGRRGKRIVELNYAAIDAGSHGIIEVKVDPEWKKL
                     KPLRTVELMGDKYFDEYVNPIDNLEGYDLPVSTFTKWDLLDGTMTNDITFKQKRSIAT
                     MVPRWVKENCIQCNTCSLVCPHATIRPFLLDEKEVAGLPENVREETVAAMGGPSVAGL
                     RFRIQVSPMNCVGCALCVVTCPGRGGNKALEMVEARSQFDIEPAADYLYKQVTYKTDK
                     FPLNSPKGVGFLMPYMEISGACAGCGETPYYRLITQLFGNDMLVANATGCSSIYCGSV
                     PGTPFTTNKDGEGVAWANSLFEDNAEYGFGMRMANDYKLKLIADIFTAALPKVEPELK
                     ELLEKYLANPRDRKEVRSYLPRLIELLEKSKDKGVKEALKYKDDILDKSVWIIGGDGW
                     AYDIGYGGLDHVLASGANVNVLVLDTEVYSNTGGQMSKATQTGAIAKFAASGKTGAKK
                     NLAMMAMAYGDVYVAQVALGANPVATIRALTEAENYDGPSIVIAYSPCTEHGIKGGLS
                     NHIAVEKKAVASGYWPIFRYDPRLEAAGKSPLTWDMKEPDYTQFREFVMGETRFSQLP
                     TVNPENAEFLLSKSEEEAKKRYFRLKKFE"
     gene            complement(36752..>37802)
                     /locus_tag="PK460_01370"
     CDS             complement(36752..>37802)
                     /locus_tag="PK460_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53784.1"
                     /translation="ESSAEIPSASTAPLVETLVRTIDFEELDAGNMSATHHNTYYDGL
                     IVADGRVTTTKKTSIDNKSLGMRMPASTTEEPRICTIKVSGYTKIVLDLKQERTHTRI
                     KTNTGAIYQGTKNEDILRDVEILTTELTELVFTFYTETLISSTMDCGFDNIRFYALVE
                     EGGLPPSSETPTSSEPISSSEETSTTSEIPTSVDGTYDYITMRDIAEFLQTKDAHYPI
                     SDAFIKEYQQKTGGYIDYARQPGKVVDKSICEPDQKWHFFDSWLYRTLEEGGITWTDS
                     AKNRVYTKLLCPELLLWIYEACAAPEAKILAAYDAAVAGKEAGTHISTIAKNMRACVP
                     WEDLEINIQYFLDNK"
BASE COUNT        12096 a         7055 c         7889 g        10762 t
ORIGIN      
        1 aatgcaccaa ttcaaaagtt tgccgctaca gttaaagcgg tcgcagaaaa acaataaaat
       61 tgcccaagga ggaaaagaaa aatggcaaaa ttaactgtta cagacattat tgcggcatta
      121 aaagaaatga ccgtactcga attaaatgat ttagttaaag cagtcgaaga agaattcggc
      181 gttaccgcag cagtcgcaac tgctggacca gcaccagaag atgatggccc agttgacaaa
      241 ggtccatcag aagtcacctt agtgctcaaa cactggggtc aaagtaaagt tgcagtcatt
      301 aaagctgtcc aagctatcac tggcttaggc ttaatggatg ccaaaaaatt agttgatggt
      361 gcccctgtca acattaaaga aaaaatctca cctgttgaag cagaagaaca caaaaaagct
      421 cttgtcgaag ctggtgcgga tgttgaaatt aaataactta aataagcaag atgcgaagac
      481 ccacaattca tgttgtgggt tttcgtgcgt ttaaggaagg ttgaatttga atcattattt
      541 tgcaaataat aacgagctag aacatgatat tcgcacatta gaattatcat tacaaggcac
      601 aaatttaacg ctaattactg atcgcggagt cttcagcaat aaagaaattg atgaagggtc
      661 cattgtacta ctcaaaagta ttattaagag catagacgta aagggcgaag tacttgatgt
      721 cgggtgtggt tacgggacgc ttggcttata tttagccgca cgctaccccg aaattaaagt
      781 cacaatgttc gacattaatg aacgcgctgt aagtctaacg agtgagaacg ctaaacgcaa
      841 caacctcaca aatacacatg tttatgtaga tagtgactat caggctctac ctaaacatac
      901 attcaacttg atagttatta acccaccaat tcatgcgggt aaaaaagctt attaccccct
      961 tcttagtgcg gcgcgtgact atctcactga tggcggaata ctgatgtttg tgattcgtaa
     1021 gtcccatggc gctaaaagtg ccgcaaagta cttaagtgac tattattcaa atatcacact
     1081 tatacaaaaa gatgcgggat tctacgtcta tcacgcacaa aagtaacaaa atacaagtaa
     1141 agcgaggcca tttatgaaaa caattaacga taaaacaaaa agactccaca atgaccgagt
     1201 tgactttagc aagatcagcg gaaacttcga agttcctaat cttgtaggaa ttcaaatcga
     1261 ctcattcgag tggttcaaaa atgaaggtat cgcggaagta tttaatgaaa tcttccctgt
     1321 tgaatcttat agcaaagaca ttgaaattgg ctttgaagga ttccgctttg atactccaaa
     1381 atatacgtat ttagaatgta aagatcgtgg ctttacttat agcgcaccat tacgcgttac
     1441 attatcgctt gggattagaa aaacgggtga agtcgttaaa agtgaaatct ttatgggtga
     1501 tatcccttta atgacacata ccggtacatt cgttattaac ggtgctgaac gagtaatcgt
     1561 ctcgcaaatt gttcgttcac caggcgccta tatgtccaaa gagttagaca ctaaatcagg
     1621 taagtttatt tataacgctg atttaaatcc aacgcgcggg acatggttaa actttgaaaa
     1681 tgacgctaaa ggtttattat cagtccggat tgataaacaa cggaaaatga atgcaacgtt
     1741 gcttttaaag gcacttggca ttaccgatta tcacgagaca attgaactct ttgatgtgtt
     1801 tgacgcgaaa aaccgcgcca aaaataataa acgtaaatcg gaagaagaga tgacttcgct
     1861 tgaattaact ttgcgtaaag acgaaagttt tacaacttca cttgaagcac tttacgaaat
     1921 tttccggcgc ttaaaaccag gcgaaccaat gagtgatgaa ggggttaaaa atcaccttat
     1981 tcaaaaattc tttgatccaa agcgttatga cttaggcaaa gcgggtcgct ttaaatttgc
     2041 gaaaaaactt ggtatctacg aacgtttaga aggcgttgtc cttgctgaag atttagtaag
     2101 tgttgacggc gaaattcttt tcgaaaaaga acaccaaatg aaaaaagaag atgtcgataa
     2161 gctccgtgcc cttaaattct tcgaaaaagg tgcccatact tatacgattc cttttaaccc
     2221 tgatttagat gattataata ctgtaaatat tgttaaggct tatgttaatg atgaaaaaac
     2281 acaagcaatt aatattgttg gcactgattt aaatattgac gaaaaacgcg tcacgactcc
     2341 tgatattgtc gctgttttct cctatttctt aaatctaatg cacggtattg gtcgcgttga
     2401 cgatattgac caccttagca atagacgtgt tcgtccagtt ggcgaattaa ttcaaaatca
     2461 attccgtgtt ggactttcaa aaatggttaa agcaatgaaa gattcttcat caattgctga
     2521 tacagaaact attactgtcc aaaaacttgt caatactcga gcagtagcga gtgcgattcg
     2581 tgatttcttc gcgacaagtc agctttcgca atttatggac caagttaacc cacttgcgga
     2641 acttacaaac aaacggcgta ttagtgcctt aggtcctggc ggacttaccc gtgaccgcgc
     2701 aagctttgat gtgcgtgacg ttcactattc gcactatggt cgtatttgtc cagttgaaac
     2761 gccagaagga caaaatattg ggcttattaa taacctagcg tgttatgcga aagttaatga
     2821 atatggcttt attgaaacac catatcgtaa agtcttaaaa ccatatggtc aaattacaaa
     2881 cgaaacaatt tatttagacg cggacgaaga aaaagagttc gttattgccg aagcaaacgt
     2941 tgaagttgat gaaaatggtg tgattcaaac tcaagaaatc atcgcccgtt atcaaggtga
     3001 aaacgtctta gttagtcccg ataaagttga ttacattgat gtctcaccaa aacaaatcgt
     3061 gagtatcgcc gctgcgtcaa ttccgttttt agaaaatgac gacgcaatgc gggcactcat
     3121 gggtgctaac atgcaacgac aagcgctacc attaatggtg ccagaatcac catatgttgg
     3181 tacgggactt gaaacaaaaa ttgctcacga ctcggggtca gcaattactg cacataaaga
     3241 tgccgttgtc acttatgtag acggcgctaa cattagtgtg aagtttgatg acgacacaac
     3301 tttcacttat aaattacaaa aattcgcgcg gagtaaccaa ggaacttgct ttaaccaagt
     3361 accaatcgtt aaagttggtg ataaggtgaa aaaaggtcaa gttttagccg atggaccatc
     3421 gatggttaac ggtgaactct cccttggtcg taatgttact atcgccttta tgacgtggga
     3481 tggttacaac tttgaagacg cggttattat gagtgacaga ctcgttaaag aagatgtgta
     3541 tacgagtatc catattgaag aatataaaat tgaagcccgg aaaattaaaa ctgtttctga
     3601 acggattacg cgcgacattc caaatgtgag tgaagccgca aaagcgttct tagatgaaaa
     3661 cggtattatt attcctggtg cggaagttaa agaaggcgat atcttagttg gtcgcgtgac
     3721 accaaaaggt caacgtgaac aaagtccaga agatcgctta ctacaaacaa tctttaatga
     3781 aaaagtgcgt gaagttaaag acacttcctt acgtgtcccg catggtggcg gcggaacggt
     3841 gattgacgtt aaagtcttta cccgtgaagc aggcgacgaa ctaggaccag acgtcgaaat
     3901 tagtgtcagt gtctttatcg gccaaaaacg taaaattagt gaaggggaca aaatgagcgg
     3961 acgccatggt aataaaggtg ttatttcgcg gattctccca gaagctgata tgccgtttat
     4021 ggaagatggc acaccaattg atattatgct taaccccctt ggtgtaccaa gccggatgaa
     4081 tatcggtcaa gtacttgaaa ttcacttagg tatggcttgt aagaaactcg gtgttaaagt
     4141 tgcttctcca gtatttgacg ggatgactaa tgaagaaatc tttgaattaa tggaagaagc
     4201 aaagatgcat aaagacggta aagaaatact atatgacggc cgcaccggtg accgttttga
     4261 tgaacggatt agtgttggtg tcatgtactt aattaaactt gtccacatgg ttgatgataa
     4321 gttgcacgcg cgtgcgactg gaccttatag tcttgtcaca caacaaccac ttggtggtaa
     4381 agcgcaaaac ggtggacaaa gatttgggga aatggaagtg tgggcacttg aagcttatgg
     4441 tgccgctaac attctccaag aaattctcac aatcaagagc gatgaccgtg ttggtcgtaa
     4501 acgcacttac gaagcaatcg tgaagggcta cgacttacca acaccaggaa tccctgaagc
     4561 atttaaagtg ttagtgcgtg aactacaagg actcgcgatt aacgttgaat tacggatgaa
     4621 agacggcgaa atccttgact tagataaact cgctaaagag caagatgcag atatccgtcg
     4681 ctcccgcaga agtactgctg ttagagaaga acaagaagtg ttcgacccag aagaaacagc
     4741 agtggtctac aaaaatgaag aaagagggga tgaataatta tggcaagaaa acaaccattt
     4801 aatattaatg atttagcaag tgtccaaatt ggacttgctt ccccagacat gattcgggaa
     4861 tggtcaaatg gtgaaatttt aaaaccagaa acggtcaact accggagcca aaaacccgaa
     4921 cttgatggtc ttttctgtga acgaatcttc ggacctagta aggattatga atgtcattgt
     4981 ggtaaatatc gacggattcg ccaccgcggt acagtttgtg aaaagtgtgg tgttgaagtt
     5041 atttctaaat atgtgcgtcg tgaacggatg gggcatattg aacttgctag tccgtgtaca
     5101 catatttggt acttaaaagg gacaccggca agaattgcaa cccttttaga tatccctgct
     5161 aaacaactag aagaaatcgt ctactttatt agccatatcg taactgatcc aggcacaagt
     5221 agtgtccttt cttacaaaca atttattgat gaaacaacag ggcgtcaagt gttcgctgaa
     5281 gcgattaaag aaattcaaga aaatattgaa gaaggtagct ttgaacacgc aaaaggtagt
     5341 gaacttttag aacgacttga agtgacacaa actttcgact tctttgcgac ttcgcgttat
     5401 attagtaaac atactggcgc taagtttggt gaaggtgcgg aagtcattaa gcaactctta
     5461 aaagaagttg acttacaaaa agaatttgat gaagttaacg ctgcacttaa aaccgaaagt
     5521 aaaacaaaag aaaagaaatt attacgacgc ttagaagtaa ttaatgcttt ccttaaatca
     5581 ggtaaccgcc cagaatggat ggtgcttgat gttattcccg taattccacc agatttacgg
     5641 ccaatgttac aacttgatgg cggcagatac gcgactagtg acattaacga cttatatcgc
     5701 cgtgtcatta gccggaataa ccgctataaa gccctagtgc aaatgaatgc gccaaaagtt
     5761 attctcatga acgggcaacg gatgctccaa gaagcagttg atagtctcat tgataacggc
     5821 cgtcgcggca aaccatacac tggcgcgaat aaacgtccac ttaaatcatt aagctcaagc
     5881 ttaaaaggta aacaagggcg tttccgtcaa aacttactcg gtaaacgcgt tgactatagt
     5941 gggcgtagtg ttattgctgt cggtcccgaa cttaagatgt atgaatgtgg tatcccgcgc
     6001 gaaatggcga ttcaattatt ccgcccattt attgctgcag aactcattaa aagagattca
     6061 cgcgccacaa atcatacaca agccgataag attattgatc gtcatgatga aattgtcttc
     6121 gatattattg aagaaattat cgctgatcac ccagtattac ttaaccgtgc accgacgctg
     6181 caccgtttag ggattcaagc gttccaacct gtgcttgtca gtggtcgtgc gattcgttta
     6241 cacccacttg tgtgtgcggg ctttaacgca gactttgacg gtgaccaaat ggccgtccac
     6301 gtcccgctta gtgaagctgc gcaagaagaa gcgctaaact taatgcttgc gagcaaaaac
     6361 attttagggc caaaagatgg taaaccaatt gccgccccgt cgcaagacat ggtgattggt
     6421 aactacttct taaccgttga agagtcacgc gaagactttg tgcgtcgcgc cgaagaagca
     6481 cgagcgttag gcgacgaatt tggtgcgcaa aagtttatgc aatatgctga aagcgaaggg
     6541 aaagtatttg gttcgattga tgaattaatg gttgcttatc aaacaaatag tgtccactta
     6601 cataaccgta tcgcgcttcc tggccgagca tttaacaaaa cattatttac accagaaatg
     6661 aataagtctt atcttgtgac aagtgttggt aaagtcattt ttaatacaat tttccccaat
     6721 gacttcccat acttaaacga accaaataaa ggctcattac aaatggataa aattgaatac
     6781 tttgtgcccg ctggtacaaa tattcgcgaa cacattgcaa gtttaccgct gcgtaaacct
     6841 attaaccaag aattcttagg tttaattatt aatgaagtgt ttacgcgtta tggcacggtg
     6901 aaaactagtg caattcttga caaaattaaa gaccaaggtt tccattattc aacaaaagct
     6961 ggtcttactg tttcactcca cgacattacc ccaattgaag gtaaagaaga actcgttgaa
     7021 gctggtgaag ttgaagtgcg gaaaattaat aaagccgcag cgcgtggatt ctatagtgaa
     7081 aaagaacgct ataatgccgt cattaatgtc tggaaaggtg ttaaagatga cgttgaagct
     7141 aaacttaaag aacagttccc acttgataaa cgtaatccgc tctacatcat gtttgatagc
     7201 ggagcaagag gtaaaacaac aaacttctta caacttaaag ggttacgggg cttaatggct
     7261 tcgccgcgtg gtggcgaaat tgaaattccg attaaatcaa acttccgtga aggcattagc
     7321 gtgagcgaat tcttcattgc ttcgcacggt tcacggaaag gtggtgcgga taccgcgatt
     7381 aaaactgctg actcagggta cttaacccgg cggttagtcg acgttgccca aaatgttatc
     7441 gtcaccgaag aagactgtgg tagtgatcac ggctttgtcg ttaccgaaat tcgtattcgt
     7501 gaaaatgaca ccccagcagt gccacttagt gaccgtattg cgggtcgcta tacagtccat
     7561 gacatcattc acccgacaac tggtgaagta attattgaag cgaatactct tattaataaa
     7621 gacgctgcgc tttatattga aaaagcgggt gttaaagaag ttgaaattcg ttcgctcttt
     7681 ggatgtgaag cgccaagcgg aatttgtgcg cactgttacg gcacaaactt agcgactggt
     7741 gaacgtgtta aagttggtga agcggtcgga attatggcgg cacaatcaat tggtgaacca
     7801 ggaacacaat taacaatgag aacgttccac agcggtggtg ttgcgcaagt tgaagacgtc
     7861 acccaagggt taccacgggt tcaagaatta tttgaagccc gtaatccaaa aggtaaagca
     7921 ataatcaccg aaattgctgg tgaagttgta agtatcacgc aagaagaaga ccgtcgctat
     7981 accgttactg ttaaaaacga tttagaagaa aagagttatt taacagagta tggtcaacaa
     8041 ttacgtgtta aagaaggcga cacacttaaa caaggggacc gcattactga aggtgctgtc
     8101 cacccgcgtg aacttctcgc tgttagcaca attaccgcgg tgcaaaacta tttacttaac
     8161 gaagtacaaa aagtttaccg catcagcgct tctgtccgca ttagtgataa gcacttagaa
     8221 attattatta accaaatgct caaccgcgtt tatattattg atggtggtga tacgagtctc
     8281 ttaccaggac aaagaattac gacccatcaa tttaccgcgg cgaataaaga agcacttatt
     8341 aacggactcc atccagctgt agcgacacca ttaattcttg gtattaaagc tgctgcgctt
     8401 gcaagtgact ccttcttaag tgccgcaagt ttccaagaaa caacacgcat cttaactgac
     8461 gccactgtta aaggtaaact tgacccactc cacggcttaa aggaaaacgt cattactgga
     8521 cgcttaattc ctgctggtcg cggtatctta acgaaagaag agcaagaggc cttgcttgaa
     8581 gactttagtg tcgctgacac tttagacaaa attaaagcaa gttacgacaa tagtagtaac
     8641 agctaaataa attaagggag caatataaaa acatattgct ctcttttagt aaccggtttc
     8701 atataaatat atgaacctta tcatttaact agtaattaga gcatgatata atgccaaagc
     8761 cattaaaaag aaaggatggt atgattatga aaaaaagatt actattatta ccactattag
     8821 caatgcttct aggtgggtgt aacggcggaa gtagtgctac tccaagcatc agcaccccag
     8881 aaagcattcc tagtagtgaa gaaccaagtg ttgaatcaag tgcagaaagc gtcagcgttt
     8941 caattgatac gacgagctat gaaatagcga caacgacaaa ttattcaaac cgtgaattag
     9001 atcattcaat tgatatgact gcattagact cacttggcac acaaaagatg ttagtcgttc
     9061 ctgtcgtgtt tagtgacacc gcgcaccgcg caactgaaga agtccatacg atgattaata
     9121 aagctttctt tggtgcaagt gaagaaacag gctgggaatc agtcgcctct tattatcaca
     9181 aaacgagttt tggaaaatta actttaactg gtgaagtact cccttacttc tattcacaat
     9241 ggtcaaccca tgcttttggc gcattagaag tttccccgac gcaagaatac ggtgaaggtt
     9301 attactggga tcaaacgcac cacttaattg aagaaattta caatacatta gatgccgaac
     9361 tattcttaga atatgaccaa gatggcgacg gccacgttga tgctttctgg atggtgtata
     9421 ttgccgagat taaagcatta ggcggggaag acgatccgtt ctgggcttat aaattttact
     9481 ggaaccgtta cgctaatacc gaaaaaccaa caccaaatac ttatgcgtgg gcaagttata
     9541 agtttgctga cgaaggtgtc ggttataact tcaatattcc tgatgcgcat acttatattc
     9601 acgaaacagg acatatgttt ggcttacctg actattacga ttacgattca aaaaccgcac
     9661 cagcgggcgc gcttgatatg atggatcata acgttgccga tcacaatatg tattctaaat
     9721 atcgcctcaa ctggtcaaaa ccatttgtcg ttactggtaa tgcggatatt catctccgtc
     9781 cagcggaatc aaccggtgac tttatcctta ttaaagacgg atggaacgga catgtgtatg
     9841 atgaatatat tttaattgaa tactataccc caaccggact taatgaaaaa gatagtcaag
     9901 ggagggggta tgaaacaaaa tcaagtaacg tatcgacaaa aagctttgat attaatgggg
     9961 tgagaatgtg gcacgttgat tcacggctca tgacttatgt cttcgatcct gaatcattag
    10021 aattaacaaa tgcattttgg actgatgaaa ttgttgttgg tgatccattt agttacacta
    10081 ttattggtcc aagtaataca acttcacgta gcagcacacc agaaggcgat agcaaggtta
    10141 ataagttatt acacttaatg gacgctaccg gtacaacttc acgtgttggt aactggttac
    10201 ggacaccaac cactgcgggc tcaacagcgt tattccaaac tggtgatgtc gtcgccgctg
    10261 atgggtggaa gaaatatatc caaacaagcg caacatttaa cgacggcaca gcagtgggtt
    10321 acagtgtcga gattggtgaa atggatgata acggtgtcca aatcatcatt cgtaaagctt
    10381 aagctactgc ttaacaaata agcgagttca ttgaaaaacg catacatttc tcttaaaata
    10441 taaggatgaa atgtaaagtt aaaagaaaca caaagaaggt caccttactc ctaagtctct
    10501 tgctacttag tagttgtaat ttggaggtga ccttttttcc ttacacacac catataagtg
    10561 acactgatac ttatacaagg atcgaaacaa gtgattatta taaagttggt gcttttgcgc
    10621 gcagttatca agatgtatta aaaagtaatt taaatggtaa ttcacgtgtt gtaccactta
    10681 agagtagtgg cgaacaaaag atccttgttt tgccggtgaa tttccctgat tatgacttaa
    10741 atgaattaga tggtaataat gggaaaaccg cgcatattca tattcaaaat gctttctttg
    10801 gtgaaaatca cacaacacgc ttttattcag ttgcggggta ttacgaagct tctagttacg
    10861 gcaaacttaa acttagtggt gatgtggcgc cgtggtacac tttacccgct gaatatagcg
    10921 ttagcgcgat taaaagcaga gtacggagca acagcgataa acttaatgaa acggcagaaa
    10981 tacttaattt agcgctcaaa gaatacgcta aaagtaatga cttaagtgcg tttgatttag
    11041 atgaaaatgg tgtgcttgac agtgcttatg taatatatag ttatccgttt gaaccaaaag
    11101 atgtgaataa tatcttctgg gcttttgccg cgaacatgaa taaaaaagat ccagaactta
    11161 cccatcaagc gaatgcttat agttggagta gttattatta tttagatgta gcgcgcaata
    11221 aaaaacctga tccccatact tatattcatg aagtaggcca tctttttggt ttacctgatt
    11281 attataatac gaattatgac gatccttatt ccccgcttgg cggctttgat atgatggatt
    11341 atacgcttgg tgatcatacc gggttatcaa agatgttact tgattgggcg cggccttacg
    11401 ccttaaaaag aagtactaca attaaacttc gaccctttag tgaaacaggt gacttactcg
    11461 ttcttaaaaa taactggaac gagaaagcaa atgatgaata tattttgctc gaatattaca
    11521 caccaacagt gttaaatgag cttgattcaa cgcttaatgc ttcctttaaa ctgccaaaga
    11581 caaacggagt gaaggtgtat cacgttgacg cgagaacggt gtacgaaatt aaaaacggga
    11641 gtattgttaa gtatccttac gttaatgatt atgaaggaga atttaacggt actgaacttt
    11701 tagcgcatag caatagtact ggaagttata ataatgcacc aattaagacg aataaacttt
    11761 ataaattact tgagccaaaa gaaagcacga acatcagcga gtcgtttaat caagcaaatc
    11821 agcactcttt gtttcaaaaa ggtgatgagt ttggctatga atattttaaa aatttcgcat
    11881 ttaatgataa gacaccgctt aattttcgct ttaaaatagt acaattaact aacgcttatg
    11941 cgacaataga aatagaggta tattaagatg agaaaaagta catttatccg tcgagcatta
    12001 attttagtag tcccagctct actcttaaca agctgcgtga cttttggtga tatattaaag
    12061 tcgtccgagt ttggtgcgac tagttcaagt gcgaatgaag taacttccga agtaactggc
    12121 agcgaaaatc cctatccttt aaacgggaca tacggcgtta atgattatga acacccaatt
    12181 acggcgaatc cagatacttt aaataattat tataaagtag aaccgcttaa caataattcg
    12241 cgactaatta ataactacgc tatttctaat acgatgggtt tagcgcacct tgattcagtt
    12301 tctaaccaaa aaatgacggt tgtacctgtt aaatttactg atacacccgc aagttacggg
    12361 ggagaggcga ctattcaggg agcgattaat cgcgctttct ttggtgaaag cagtgataca
    12421 ggttgggaat cagtagcctc ttattactat aaatcaagtt atggtagact cagtcttacc
    12481 ggtgaagtaa tgcccctttt tgatatgggt tattccacta ctgaattgtt agcgaaaact
    12541 gttgacggtt atcagtactg ggataagagt cactatgtgc ttgaacaggt gtacaacact
    12601 tatctttcat caacattaaa agaatacgac caaaataatg atggctttgt tgattccgtt
    12661 ttctttgtgt acttagcacc tgttgattat gaaagtgatg atacactttt ttgggccttc
    12721 caatattttt ggaatagatt tgccgataaa aacaaaccag tatttaatgc ttacggctgg
    12781 gcgagttatg aatttatggt tgaagcgcca ggttatacat atcttaaacc agcagcgcat
    12841 acttatattc atgaaagtgg tcatatctta agttttgatg attattatga ttatgacggc
    12901 actatagcgc cagcgggagc gattgatatg atggatcata atattattga tcacaatatg
    12961 ttttctaaat ttagcgcaaa ctggagcaca ccgtatgtcc cggttggtaa tagtgatata
    13021 aggcttagac ccgctgaatc aagtggtgac tttatcatta ttaatgacgc ttggaatgga
    13081 catgcttatg acgaatacat tttgattgag tactacacac caaccggttt aaatgcgcgt
    13141 gatagcttat cagtttcagg ctataacggt gtcaaaggtt acacgattcc tggggtacgg
    13201 atatggcatg ttgatgcgcg gttagtgatt ttgtcaaata acggtaatac taaaaagtgg
    13261 agtgatacaa ttccgccgca cttagatggt agcggtggtt catggatgga ttttgctact
    13321 tccaatacac caagttataa ttatgcgcat aacagtttac ataaattgca tttgcttgat
    13381 ggtcgcggac gcgcgcaaac atgggcaaat aatttagtaa aagcggacga ctcagcgcta
    13441 tttcaaacag ggacagttat tgctagtaat aattggcaaa agtacttgtt aaaaagagca
    13501 acctttaatg acggtagcgt aattggttat agtattgaaa ttggtgaaat gaatagcgaa
    13561 ggggttacca ttaaaattcg taaagcttaa gttgtcgttt tgtattgtaa tttttattaa
    13621 tcaaataatg ataaaataaa acatatggct acattaaaag atattgcaaa agctactgga
    13681 ttttctgtta ttactgtttc gcgtgtcata aattcaccgc atttagtgaa agaaaaaaca
    13741 cggaagatta ttgaagcaga aatagcgcgg caaaatttta agccgaacgt tttagctaga
    13801 gcactcgtcc aaaatcgcac gaatatcatt tatgtgtata ttcccgctga tattgatgcg
    13861 attaacccgt tctttttaca tgttgtgtct ggtataggcg aagtacttgg cgaaaaaggt
    13921 ttttccttac tcttaaaaag atcgtggtat gagggcgaag attgtgatgg ctgtattttc
    13981 atgggactta gcgcggaaga tgaagcaaag gtatttgaac tttcaaaaac taaaccagtg
    14041 tgcgtctttg gctatgtaga aggagtgtca agtgttgacg ttgataatta tcaagggctt
    14101 tacttaatga caagtcacgt acttagtaaa ggctatcgaa cactgacata tttaggaatt
    14161 gatgactata gacggtttat cttacagcgt aaaaaaggat ttagtgacgc agttatgagt
    14221 caaaaagata aaattactgt aaaatatttg gccgctaaaa ataatgaaaa tgacgcttat
    14281 aaagtcgtgg gtcaaagttt aaaacaaggt gatactcctg aagtaattgt gtgcgctaca
    14341 gatgatatgg caattggtgc tattcgcgca attaaagaaa aaggtttaga agtaccgcgt
    14401 gacatagcag taactgggtt tgatgggtta ggtacccaag aagtagcgca ccctcgactt
    14461 actaccattc atcaaccaat ttacgaagcc ggaaaagcac ttgcccatcg cattattgcc
    14521 ctgattgaaa ataaaaatga attaccagtt aatcacgcat tcattgaaac aacgattatt
    14581 catcgcgaat caatctagct attacataga atttcaattt aacttgcaaa attagcaatt
    14641 atattttaaa atgataacga taacatttta tagaggtgca caaaatgaaa ccgaagaaaa
    14701 taacaacaat cttatttagt ctttttttgg ctttatcact cagtgttgtg actggctgta
    14761 aagatggtag tgaaaatggt gggagtgatg acccaattaa cttttgggcc tatcagcctt
    14821 caaaacaaac agaccaaaca gcgtataaaa atttaattgc tgactttaca gcggaaacag
    14881 gaattaaagt taaattactg atggtcgtta aagatagtta taacaaatca cttaatgcgg
    14941 cgataagttc acgcagtaaa ccagatttag cttatttcga tcaaccacgg attagtgatt
    15001 atgcgcacga cgacacgatt gctaaactag acgaatatat cgctaatagt gcggttattg
    15061 ataatactaa attctttgct tcaggtttag cgacaacggt gtataaaggt agtcagtacg
    15121 gtgtgccact gaatatgaca acatcagtgc ttttttacaa taaagacatc gtcacgaccc
    15181 cgcccactag ttggcaagaa tgacttaaca ccccagtaag tggacataat cagtcactat
    15241 ttgacgggat tggcactgga ggatatgcgg gatggttcac tgaccctgaa ttaacgattg
    15301 cttatgttcc gggtctatta actgctgatt taacactcta cggaaaatac gttgaatcac
    15361 aagatactac tttaatcttc tatgacccga ataaagttct ttcaagtggt gacgtttatc
    15421 tttatacatg ggacggtgat ggtcgcaaaa taacaggcaa ttggcctgga acagcaatgt
    15481 ttaatcacgg attcggctac tattcatggt ttttccatgc tgcctatctt accgaaaacg
    15541 ttatattcca cgataatgct ggaaatcaaa cgggcgatat agtgtgggaa attggtaaaa
    15601 atgtctatca tcaagaaaca ggaacatgga cttttgttag tgaagttcat cataaagcta
    15661 ccgtatacgc gagtaagatt cttgaaaaaa cggcaacatg tgacgcaaca ggtgcagcaa
    15721 ataatttaaa tgcaccagaa tggaatgaaa cactagcgaa tcaatataat gactcacttg
    15781 atcctacaac aaaagcgtat ttaaaaacag cggttgctga ttctaatggt gacttattag
    15841 cgcaagcaat ggcgcgttat gactatattg tgaagaagta tggaaccgca atgtttagta
    15901 acttccttga ccggacaatc cctgctagtg gatctccgct tgtaagtaat gaaggcacaa
    15961 acaatgcact ggtaatgggg gcatttatcg ttatggcatt agttgctctt gctggcttta
    16021 aattaatcag taaaaaacat cgttatcact aaattataaa acaacgtttt atgtaactaa
    16081 aggctggggt ttgactccag cctttttaaa cattaataaa aataggttga atttatccta
    16141 ataataatct ataattatcc tgagggtgcg ataatgatta ttaaaacaga gcaaattatt
    16201 acaatgactg atgcaaatca aaatttttca aaagcttcaa aaactgcgga aaaatatggt
    16261 tccgctgtga tatttaaaaa taacaagcca aaatacattc ttgcagatat tgaacaatat
    16321 attgagttaa cagaagacga aaagatagaa atagttgcga aaagaatcct tagtaaacat
    16381 atcaacgcct ttaaaaattt agcaaaatga ttaaatttac taaagaacga atattattct
    16441 atataaactc ataattgagg ccacaggagg agtttttggt attcgtgatg agagtctatt
    16501 agaatcagca attgaagcac catatcaaac ttttgatggt gttgaacttt tcccgactaa
    16561 aattgaaaag gcagctagac ttggctttgg atttatttca aatcatccgt ttgtggatgg
    16621 taataaaaga attggcttat atgtgatgct ttctttttta caaataaatg ggattataat
    16681 ttcttttact gatgacgaaa ttgaacaaat agcctttgga atcgcaagcg gagctattaa
    16741 ctatgttgat ttaaaaagaa ttctcgaaca aaaaattgat aattaaacat attaagcatt
    16801 ataattcaca ttaaaaatca tatagttaat ttatatttta attgcatatt cttaacaacg
    16861 gattttactt tgtcaattaa caatctcgaa taattttcag ttttgtgttg acattattta
    16921 gcaatgtcct tataatatca aagtgcaatg acctaaaggt cgtttttaaa ataaaaacct
    16981 tgatacaccc ggttgtgtgt cgagtagcag aaaagaagat tcgaagaaag gagaaagatg
    17041 gatgccaaca attaatcaat tagtgcgtca tggccgtcaa aaggcagtct acaaatcaaa
    17101 atcacctgcc cttaccaaac gctggaactc acttaaacgt cgtgaagtta atcagtattc
    17161 ttcacataag cgtggtgtgt gtacccgggt tggaacaatg acaccaaaga aacctaactc
    17221 agcgttacgt aagtacgctc gtgtacggtt atcaaatggt tatgaagtta cagcctacat
    17281 tggtggagaa ggacataact tacaagaaca cagcaccgta ttaattcgtg ggggtcgtgt
    17341 taaagactta ccaggggtcc gctatcacat cgtgcgcggt acattagaca ccgctggtgt
    17401 ggaaaaaaga cgtcaaggtc gtagtcaata cggaacaaaa cgcccacata ctgctgaaca
    17461 ttaataaaaa aataaacaaa ctacttatta tcgaaggaag gaggaaacga tatgcctcgt
    17521 aaaggaagtg ttgcaaaacg tgacgtctta ccagacccaa tttatcactc aaagctcgtt
    17581 acccgcttaa ttaataaatt aatgtatgac gggaaacgcg gaacagccca aacgattctt
    17641 tataacgctt ttgaacgtat cgaagcgaaa acaaaacaac ctgcaatgga cgtttttgca
    17701 actgcaatta gtaatattat gcctgaggtc gaacttaagg ttcgtcgtgt cggtggccaa
    17761 aactatcaag tcccgactga tgtgagccca gagcgtaaaa caacattagg tcttcgttgg
    17821 ttagtgaact attcacgctt acgcaatgaa agaacaatgg aagaaagact cgcagcggaa
    17881 attattgacg ctgctaatgg aacaggcgcg tccgtcaaac gtcgtgatga cacccataag
    17941 atggcggaag ctaacaaagc ttatgcgcat tatcggtact aggaggaccc atggccgatt
    18001 taactaaatt tgatttgcaa catacgcgta atatcggaat tatggcgcat attgacgcgg
    18061 ggaaaacaac gacaacagag cgtattcttt actacactgg tcgtttacat aaagtcggtg
    18121 aaacacacga aggtgcatcc gcaatggact ggatggccca agaaaaagaa cgcggtatta
    18181 caattacaag tgcggcgaca accgcctttt ggaaagaaaa ccgcattaat atcattgata
    18241 ccccaggaca cgtcgacttt acagttgaag ttgaacgttc attacgtgta cttgatggtg
    18301 ctgtcacagt attagatggt aaaagtggtg ttgaaccaca aacggaaact gtgtggcgtc
    18361 aaggtacaaa atacaatgtc ccacgcatcg tttttgtcaa taaaatggat gcaactggcg
    18421 ctgatttcaa tatggcggtt agcacgcttc atagccgact tgccgcaaac gctgaacctg
    18481 tgcaaattcc catcggtgtt gaaagtaact tccgtggaat catcgatatt attgaagaaa
    18541 aaacatatat ttatgatgat aatatcgcta atgtttcaat cgaagaagtt ccggaagaat
    18601 acctagaaga aatgcacatt gcaaaacaaa acttaatcga aaaacttgcc gcctttgatg
    18661 acgacttcat gattcaagtc ttagaaggcg aaactccaag tgtttcagaa atcaaacgcg
    18721 ttattcgtaa agccgtctta actggtgaat tcttcccagt attctgtggt agtgcttata
    18781 aaaacaaagg tattcaacca ctgcttgacg gcgttgtcga ttacttacca agtccgttag
    18841 atgtcccgcc ggcaaaagct gaactacttg atggtactga aattattacc ccaagtgacg
    18901 ctgatgcacc acttgcggcc ttagccttta aaatcgcgac cgacccattt gtgggacgcc
    18961 tagcgtatgt ccgtgtctat tcaggtacga ttaaagctgg atcctatgtg tataacagta
    19021 ttaaaggcgt tcgtgaacgg attagtcgtt tagtgctcat gcacagtaat caccgcgaag
    19081 aagtaagtga acttaaagct ggtgatattg gtgcggttgt cggatttaaa aacacgacaa
    19141 caggggatac aatttgtgat gaaaagaata aattaatctt agaacgcatg gaattccctg
    19201 aacctgttat tgaacaagca atcgaaccaa agacccgtgc tgaccaagac aaaatgacaa
    19261 atggacttgc taagttaatg gaagaagatc caacatttaa ggtgacgact aaccccgaaa
    19321 ctggacaaac aattatcgct ggtgttggtg aacttcacct tgatgtctta gtcgacagat
    19381 tacgccgtga atttggtgtt aatgttaatg tcggtgcccc aatggttggc taccgcgaaa
    19441 caattagcca agaggcggaa tgcgaaggca aatatattaa acaatctggt gggcgcggac
    19501 aatatggtca tgtgtggatt cgctttgaac caaacccagg caaaggtttc gaatttgtcg
    19561 atgcaattgt cggtggtgtg gtcccgcgcg aatacattaa gccaaccgaa gaaggtttaa
    19621 aagacgcatt agtgcgcggt ttagtcgctg gatatcaagt aattgatatt aaggctacac
    19681 tgtatgatgg cagttatcac gatgttgata gtagtgaagc tgcttacaaa attgctgcat
    19741 caatggcgct taaagaagcg ggacgcctag cagaacccgt cttacttgaa ccaattatga
    19801 aagttgaaat tactgttcca gaacagtatt atggcgacgt gcttagtgac ttagcaagac
    19861 gccgcggttc aatgaaggat gaaagccaac gcggaaacgc taaagttatt gatgctgaag
    19921 taccactttc cgaaatgttt ggctacgtta ctgatttacg tagtttcacc caaggtcgtg
    19981 gtacatatac gatgactttt gatcactatg gcgaatgtcc acgctacatc gcagatgaaa
    20041 tcgtcaaaaa gagtggaact agtgctcgct agtccgcaaa tgctatataa atagcacaat
    20101 gatttgataa aaaattaaaa ttgctataaa ctgatagcgt tgcaataaat taaaacgcta
    20161 caagttagga ggaaattatt atggcaaaag aaagatttga cagaagtaaa gtccacgtta
    20221 acgttggtac cattggtcac gttgaccacg gtaaaacaac cttaaccgca gctattacca
    20281 aagtcctcgc atcgcaaggc ggtgcaaaat taatggcgta tgatcaaatt gaccgtgcac
    20341 cagaagaaaa agaacgcgga attacaatta atacttcaca cattgagtat caaaccgcca
    20401 ctcgtcacta tgctcacgtt gactgtccgg gacacgctga ctatgttaaa aacatgatta
    20461 ccggtgccgc acaaatggac ggagcaattc tcgttgtggc cgctactgac ggtgttatgc
    20521 cacaaacacg tgaacacatc ttacttgcac gtcaagttgg tgttccttac gttgtagtct
    20581 tcttaaataa agttgactta gtcgacgacc cagaactcgt tgacatcgtc gaaatggaag
    20641 ttcgtgaatt acttaacgaa tacggattcc caggcgatga taccccaatc gttcgcggca
    20701 gtgcccgtga tgcattagac ggtaaagcag atgctgaaaa agcaatcatc gaattaatgg
    20761 aagctgttga cagctacatt cctgacccag aacgcttaac cgatcgtcca ttccttctcc
    20821 caatcgaaga cgtcatgacc atcagtggtc gtggtaccgt cgtcactggt agagtggaac
    20881 gcggaatggt caaattaaac gaaccagttg aaatcgttgg tattcgtcca actaaatcaa
    20941 ccgtcgttac tggccttgaa atgttccgta aattactcga cagcgcccaa gctggtgaca
    21001 acgttggtgt tttactccgc ggtgtcgcac gcgacgaagt cgaacgtggt caagtcttag
    21061 ccaaacctgg ctcagttaaa ccacacacaa aattcacaag caccgtttac gtcttaacaa
    21121 aagaagaagg tggtcgtcac actgcattct tatcaaacta tcgtccacaa ttctacttcc
    21181 ggacaactga cattaccggt gttgtaacac taccagccga tgtgcaaatg gttatgcctg
    21241 gcgataccgt caccttaacc gttgaactca ttcacccagt cgccattgaa aatggaacta
    21301 agttctccat tcgtgaaggt ggccgcactg ttggtgccgg tactgttacc gaaatcttaa
    21361 aataagattt aaagcattac acaactaact cctcttcaga ggagtttttt gtttatgatt
    21421 atatcgtaac aattgaaata tatgatagtt tttagtagca tagtacttag cgaaatatga
    21481 aaaaagaaaa gaaaattacc caacctaatt tagatacagt tccaggtggc gctttaaagc
    21541 gtactggtgc agttttaatt gaccttgtca ttatgtattt attaatgaat gtattatggt
    21601 ttgccattca tccaatttat aacaaagttt atgatattga taatgttgtg accgcacatg
    21661 agcgtgataa aaaacgtagt aacttagtcg tccttaatga accgattctt gatgatgaaa
    21721 gcaatattga cttagttaaa gttgaaagta atcaattacc agaaacaaaa cgcggggaag
    21781 gtgtctttaa gttttacaca gtctttttgc gcagtgaaca agaagcgaaa ttagcggcgg
    21841 gtgaaattga taatacacct gaaaataatg caatgtttac atttgattgg tacttagaaa
    21901 atgtcattaa acctgatacg gaagaatcaa tttatttacg cattcccact gatgaacatt
    21961 taggagcaga aggcgcggtt agtagtgaag aaactgctga accggagtac gacacttttc
    22021 gtccagcggg attagatttt aaagcggatt taactgcgga agatattaat aaatttaata
    22081 accgcattta caatcaagca gtgaacgctt ttaatttacg accgaatatg acaatcatta
    22141 ataaggttaa tattcaggaa aatataatct tgatggtgct tggtagcact gtcgtctttt
    22201 tagtgttccc gcttttcttt aaacacggtc aaacactcgg caaaaaggcc ttaaaattag
    22261 gagtaacgac gcgtcatggt tacacagtta ctgtgccgtg gttattactt cgttactttg
    22321 ctttcttatt tattaactta ttaagtaatc tcttaattcc aattatttta ccgtttatct
    22381 cactcacaat tatgaccttt agtaaagagc gtcgcagtgc gcatgacttt atcgctaata
    22441 cgaaagtaat tgatttacaa aattcgacag tatataaaag tgaagctgag ttcttagaag
    22501 cacaaaaaag atataatcct agacttgagg ctgaagactt taatgaagaa gtatttagtg
    22561 aacgcttcga tgaagaaagt ggcgcctaat gaaccattat gtcgtaaagc aaacagcagg
    22621 taaacgcttc cttgcgggtt tccttgatac gatatttgtc attatattgg cgcttttttt
    22681 atatattccg gcgcgcacta tagtagacaa taatggctta atcgaagtgt ttgttaaaat
    22741 cacacaaatt caattaaaaa gcggactata tgacgttgaa gggattgata atgcagtacc
    22801 ttacatcatt aatgatgttg aaaagttccc taaagcactc tacacatttt acgttgatga
    22861 aaatcttgaa acaggcgaaa ttgaacgcgg ttacgctgat atattagtgg aaggtcatga
    22921 atataacact gcagaagatt attatgtcgt tattttaggg aaaggtcgcg aagactcttt
    22981 atttactttt gacgctatta atagtgaagc gccgtgggaa gttcctttta aagaagggaa
    23041 gcaagaagcg gtgaagcaat tttacactga tgaaatagaa aaagcgcatc aacatcttga
    23101 ttatcatgaa gtagtagttc cgctcattat taaagctgaa cggcttattt tttatgcaat
    23161 ggcgggaagt tacttaacaa gtgctttaat tcttattgtc attattccaa actttaggaa
    23221 agataaagta acatttggta aggtaattac ggggaccata gtcttaaatc gtttaggata
    23281 taaaatgagc gttttgcagg caaatatgcg caatttcgcg atatttatct ttagttttgt
    23341 ctttttcttt gtaccctttc atctcatttc ctttgcgatg acactctttt ctaaaaaaca
    23401 acttagtatg tatgatcagc ttgtggcaac actcgtggcg gataaaaaag caacgcttgt
    23461 ctttaaagat gtaaatgaag aaacaaagta tcgtaaagag ctcgcacaac gcctaattgc
    23521 cgttgataaa cgcaaagccg aaagtcgtga agaaagatta cgcgataaag agaagcttaa
    23581 taaaattgat taaaaactac ataatgccga taataaaaca atagccaaaa accttgatat
    23641 tgtgctatta taaaataaac atagcagaca atctgcagga ggaaatatgg aagttagatt
    23701 agataatctg tcgaaacact tccctggagt caaaggcgct ccgacagtca aagctgttga
    23761 tcagatgaat tttactattc ctgatggaaa gcttgtcggc cttttaggac cttcgggctg
    23821 tggaaaatca acgatactct acgtgatcgc tggattacac aaaccgaccg ctggtaagat
    23881 ttggttcggt gatcgtgatg tcactgaggt tccgcctgaa aatagaggga tcggtttagt
    23941 gtttcaaaac tatgcgctct atccgcattt tacagttcgg caaaacatta cattcccact
    24001 cgagaatgtc agaccgaaac tagaaaaaac cgaaatggag caaattgctc aagaagtcgc
    24061 aaacttagtc gacattggta acttgctcga ccgcctacca aaacaacttt cgggtggtca
    24121 acaacaacgg gtagcgattg cccgtgcgct ctcgaagcgg ccccaagttt tattacttga
    24181 cgaaccgctc tccaacctag acgcacgctt acggcttcaa acccgtgaag aaatccggcg
    24241 gattcaacgt gatacaaaag ttacaacaat ctttgttact cacgaccaag aagaagcaat
    24301 gagtattagt gactttatcg ttgtgatgcg tcgcggtgtt gtccatcaaa tcgatgcacc
    24361 gcaaacggtg tatgaagaac cagtcaactt atttgttgcg aaattcttag gctcaccagc
    24421 gattaatatt tttgatggtg aattaaagaa tggtaaagta ttacttcacg gcaatgttta
    24481 tcatgaaggg cttaaatcaa aaggtaaatt tgattacttt gataaaagaa tttatgatat
    24541 cgctgttgaa ggtggttata acggaacgaa aactgattac ttcgctagct taggcgttga
    24601 ttatgttgaa gcagagccag aagttaaaga agaaccagaa aagaaagcat ttaaagaaaa
    24661 agttagtgat ttctttgcca aattaaatcc aaagaaaaaa caaaaatttg ttccgcgtat
    24721 tattgaaggt gaagttaacc ttgaaagcat ttatgaagct atcgtcggtg gcggctatcg
    24781 ttatgcttac gctgattttg aagaagaggt tggtaaactc atcgttgaag ctggtgcaaa
    24841 tcgtgatgtg aagattggtg tgcgtcctga agcattcacc cttgacaaaa atgataatcc
    24901 agcaattgat attgaagtac tttatattga acatattggc cgcgatatta cgatcgtcgc
    24961 taatgttgtg ggacaaaaac accggattcg cgttattatc cctgctgaag aacgtcataa
    25021 agtcaaagac aaagtaatta aggtatatcc aaaacgcttc tatctctttg atattgatgg
    25081 ttcacggatt ttataggaaa acgcgatgaa agattcttat ttacgaaagg aggtaggcta
    25141 tgagcactaa agcaggagta ctgcatcttg aaaaagggcc aatgcaaacg aaaaagacga
    25201 ttgcaatgcc aatttggtta aaagcaataa ttgctttatt acctgccctt ttcttcttgc
    25261 ttgtctttac cattttcccg atttttaata cagcatttat tgcttttgtt aaagactttt
    25321 atttccaaag cgggaccggt agttttgcac ttgcaagtta cttccgacaa cttgacatgc
    25381 ataataaccc tgattggtgg cgcgaatatt ttgcgattat tgaacctgat gttccctttt
    25441 tacaaccggt taaaccaatt attggttttg cgaactttga atatgtttta aaggatccgc
    25501 tttttagtaa cgcgcttatt aataccgcta ttttaacaat catttccgtc ccactgacga
    25561 ttatgatttc acttttactt gccgtatcgc ttaattccat taagccgtta cgcgcaactt
    25621 atcaaacaat tttcttcctc ccctatgtca ctaacggcat tgccttaggg atggtcttta
    25681 atacgatctt ctcgcatgag gttgggggga ttatgaacca atttatccag ttctttggcg
    25741 gaaaaccagt atcgtggatt cgtccgttat ggagtccaga aggaacgatc ccgttatcaa
    25801 aattctcctt aggatttgcg attactgtcc aatcaatttg gtcaggctta gcgtttaaaa
    25861 tccttgtctt tatggcaggt ttagcaagta ttgacaaaca atactatgac gctgcgcgga
    25921 ttgatggctc aagtcgcagc acaatcttta gacggattac ggttccactt ttaagtccgc
    25981 aaatcttata tattacgatt acttcattta tcggggcatt taaagcttat caaagcgtta
    26041 tctcgattgt ggggggcgga ccaattcact ttgggggcga aaaccgcacg atgtggatta
    26101 cggttgtcgg gtacatttac atgaaccgca acgttgaaaa gaacattcca cttgccgcag
    26161 caggttctgt cgtcttactt attgtaattt tattcatcac gcttattcag atgcaagtct
    26221 cgaagaagcg ggtgcactat taataggagg gtagcgtaat gacagataaa gataaaaaag
    26281 tcacagaagc agtcgaatta actgccgaag aagaagtgaa attatcacac attgatgacg
    26341 aacgctttca gccatcctat gaaatcgcaa aagaatataa aacgcgggcg attgttgtta
    26401 agattattac ttacacattc ttaacgcttt ttgctttctt tatgctttta ccgttctggt
    26461 ttatgattag tgtttcagtt aaaactccac ttgaatatca acgtgaagtg tcgactggat
    26521 tacgcttatt tacggcaaat ccaagttttg ttaactttag gattgtctta gggatggatt
    26581 taaaagatcc actaaaagag atgaatgaag cactcttaca tatcgttgaa acttcaccag
    26641 aacgcagttt tatcccgtat tttcttaata cgattatcgt cgcggttgtc tcaacggtct
    26701 ttacaattgt aacaacggtg cttgcctcgt ttgcgtttgc tcgtcttgaa tttaaaggga
    26761 aaaacttctt atttgcgatt ttactcgcaa cgatgatgat tcctggcgaa atgatgctta
    26821 ttacaaatat tcaaacagcg aaatggttag gatgggaaaa cacttatcgc gcacttattt
    26881 ttgtccacgg cgttagtgtc ttctacattt tctacttacg acaaacattc cagcaaatac
    26941 caaacgaatt attccttgcc gcgaaagttg acggctatgg tgaatttagc tacttatggc
    27001 gcgtgatgat tccaattggc atgccgacaa tcgttacgat tattattctc aacgttatgg
    27061 gtagctggaa cgcctttatt tggccaacac ttattgcgca aggctataac cccactattc
    27121 agaaattatt taaatataac catacgatgc gacttgttag taacgggtta atgtcgacat
    27181 ttactggtca attcgctagt tacgacaccg ttaaaattgc cgcatcgatg attgttactg
    27241 ctccgttact gctcgtcttt gtgttcttcc gtaaatacat tatgagcggt gtctcccgca
    27301 gtggtattaa aggttagtaa ttactaatcg ttattatata aacaagaaag gaaagagaga
    27361 tatgaaattt agaaaactca tatttacagc ggtatcggtc ttagctttaa caacgcttgc
    27421 tggttgttta cgcccaccga tgccaccaac atcagaaacg ccaacgacat cttcaggcgg
    27481 tggtggtccg actgaaaaaa tcgaagtgaa gttctggaca ggatttgggg catcagttac
    27541 tggtgcatta aacccgcttt tcgcgcgttt tgaacaagaa aatcctgata ttaaagtact
    27601 ttatgaatca aagagtggtt atccaaacct ccaacaagcg attaacggtt caattagtac
    27661 aaatacttat ccgcatatcg ctaatggtta tcctgaccat ttagtcggtt acattaacgc
    27721 taatatttta gtaaatcttg gtaaccccac taactacatc aatcacccag actatggtgt
    27781 tgatattaat gaatacaata gcgactacat ggccgaaatt aatggcttat cagacgctgc
    27841 aacattcggc ttaccgttta ataaatcaac ggaagtaatg attgctaacc aaagtttctt
    27901 tgatgcggct aaaaaaagag acccaagtat ctttattcca aaaacatggc aagatttagc
    27961 cgaagttggc ccaaaactta aagccgtcgc gaaagataac ggctggtttg gtaaattagt
    28021 actcaaaaaa gatggaacag cggtagcaaa acctgcggaa tttgatgcag cgcttaaaga
    28081 acaggtcgct gttgatatga cgctagttaa tgaaaatcaa tttatcccct ttagctggga
    28141 tagcgctagt aacttcttta ttacaattat tcggcaatgg ggtgcaatgt atacggaaaa
    28201 aggtaacaca attcaaaatg gttacatccg tttccaagaa gaaccgcacc gcactaaaac
    28261 actagcggcc ctatcttatt ttaaagagct atatgaagat ggattaattg gtctacctga
    28321 caactttggt gaaccgcttt attcttcaac accatttaaa gaaggaaaac tcgtacttac
    28381 tattagtagt agtgccggtg ttggtgaaaa tttaccaggc gggacaactg aatatccatt
    28441 tgaattatcg attaatccaa ttccttataa cggtgactta gcagcgcaaa aatcagttat
    28501 tgcccaaggc acaaacttag cgctctttac cgttggtcaa ggccgtgatc caaaagcaca
    28561 agctgaacgg ctcgctgcct ggcgcttact tcgcttctta acttatgaag caaactacga
    28621 atttggtaaa gcaacttcct acttcccagt gttagacgct tccaaaattg atcgcgatag
    28681 tcagcgttat caagattact taatgtatag tgaattctta aatcaaacag acgggacacc
    28741 acgcgaaaaa gtaattcgta gtaccgctaa acttcaggct aatgtgtata tggatcctga
    28801 agcaggctgg aataagttcg ttgacccagg gttccttggt tcaagtgaaa ttcgtaatac
    28861 catcgaatca gtgatggctg aattatttaa aggtaaaaca ccggagcaag tgttaaatga
    28921 cactgttact aaacttgcgc ggtatgtaca aaaataataa aggagaaata tatgaaaaaa
    28981 acatttgtta aaattggctt gttacttgcg gtttttggct tacttgttgg ttgtgataat
    29041 agcagcagcg aagtagtgcc accctcaagc gccagtgaag aagtcgtttc atcaagcgaa
    29101 gaagaagtaa aactaactga gcaagaatat caaattgccg ttaattacat tgctcgcagt
    29161 gttccgcttg tccttgaaag aggcgttaaa gttccaacaa acgttgaaag ggctgaagcg
    29221 gagaacccac taccgacacc agaattaaag tatggtgaca acttattact tattaccgct
    29281 ggtgaattaa agtatgaaga cgaaattaat ggcgtctatc tttatccaga atatactgta
    29341 acctattcat tcttagagca agaaccttat gcaggcttta cttttgaata tgatgatatt
    29401 ggccgttatt ttgctgttcc tgaatatccg cgctatgaag cggaatatga caatggcggt
    29461 aacccaattc acactgtacc accggcaaaa gcagcgcggt tatatgtcac gattcatatt
    29521 gaagaacgtt tcactacatt taatatggac atgattttag agccacaagc cattattcat
    29581 gattacaacc ttgctgatgt ccgtagtgtg ccaactggct cagttgttag agtctacggt
    29641 tactttaacg gctatttcag tgactggaat aatggtgtac tgtgtgatgg cgatgtagcg
    29701 ttaggtctct ttaaacttca agaatttaac catatggaac ttggtaagat gtacgctgtt
    29761 gttggtcaat tcacggtgta taacggctta ccacaattac aatggattaa gactgtttct
    29821 gaagttgaac ctgctgatca cccaacggtt aaagaaccaa aagctgttgt tgtcagcgca
    29881 gacgactttg ctgatcaact tgatattggt aacgaattaa gcggaccact tatgcataaa
    29941 gttggtacga cagtcgtact taacttccca cttcgttata aggcaatgct tgaccgtgat
    30001 ggtaacgccg ttgaaccagg cgcattagaa cttggtaaac acgctgatgt tgttttaacc
    30061 ggtaaagata gtgataactt tgaatttgaa gttaaacttt cacttaacta ccatatgggt
    30121 actgataatc aagcagcgtt tagagacttc tttgtcacta atgcggatgc tgatatttac
    30181 ttcactggcc cacttggtgc atataacgct atcaacttag caccatttgc ctttgaaggt
    30241 acattatcct taacccctcc tgtcgtttct taaaataaac gcatgaagct tggccccaga
    30301 ctcctggggc ctttttcatt tggcaatttt tataaatagg cgtataatta aaaagcacta
    30361 cggaccgtta gctcagttgg tagagcaatt gactcttaat caatgggtca caggttcaaa
    30421 tcctgtacgg ttcaccacgc taaaagaaag tgcccgatta tgtcgggctt ttcttgtact
    30481 tttttaaagg ataggcgtat aatttgatta ggaacaatat ttataccgaa gggaactaaa
    30541 cagaatgaaa tatttaaccg ttaaagaact tagcgaaaaa tggaagatga gtgaaagacg
    30601 gatacgagca ctcattaaag aaaaacggat tgaaggggta aaatacgaaa ataagtattt
    30661 aataccagaa aatgctaaaa agccacttga tcgccggatt aaagggcaaa gagcgttaga
    30721 agatacctca ctaaataggc aagactttga ttttaaaaac taccgcaagc actatccgac
    30781 gagtgacggc tattttggta ggtttggtgg tagcttctta ccaaagcatt tacaaaaagc
    30841 aatagatgaa gtgtatgacg gttacttaac gatcgctaaa agcgcaaagt ttattgccga
    30901 actacgcgaa attagaaaaa attatcaagg acgaccaaca cctgtgtatc attgccttaa
    30961 tttatcaaat catcttggta aagtgcaaat ttatttaaag cgcgaagatt taaatcatgg
    31021 tggttcgcat aaacttaata atgcaatggg tcaagcctta cttgcaaaat atttaggcaa
    31081 gaaaaagtta atcgcggaaa ctggagcggg gagtcacggc gcagcggtgg caatggctgc
    31141 ggcttatttt gatttagagt gcgacattta tatgggtgca gttgacactc aaaaacaacg
    31201 accaaatgtt gaccgtatta aagtgttggg cgctaatgtg attgcggtca aagaaggaac
    31261 acaaacactt aaagaagcag tagacgccgc ttttaatgcc tacgcaaaag aatataaaaa
    31321 cgccttttat gtattaggta gtgccgttgg gccgcacccc tatcctttaa tggtgcgtga
    31381 ctttcaagaa attattgggc gcgaagctaa agaacaattt gtagcaatga ctggtgaatt
    31441 accagatgtc gtcgcagcat gtgttggcgg cgggagtaac gcaattggga tgtttcaagc
    31501 ttttttagaa gaaaaagtta atattgttgg tgttgaagcg cttggtcaag gcccaacgat
    31561 tgggcaaaat agcgctgcgg tatcacttgg taaagaaatg attttgcacg gttttaactc
    31621 attagtgctg caaaatgaag acggaagtgt gggagatgct tacactatcg cgagtggttt
    31681 agattatcca ggcgttggtc cagaacacgc ttttctccat gaaataggac gcgtgaagta
    31741 cgaagcaata actgatgaag aagcagcaga agctttcttt ctcttatcgc gtcttgaagg
    31801 tattattccc gcgattgaat caagtcacgc agtagcttat gctattaaat atgcgcgcag
    31861 cgtgagtgct ggaagtattt tagtgtgttt aagtggtcgc ggtgataaag atatggaatt
    31921 tatgacgcgt gagtatccaa atatgtataa ataataattt gtgacttaaa tagtgcataa
    31981 gaaaaagcca ggtcgcccta gctttttaga atcttttaaa attatttagt taactaaaat
    32041 cgtaataaat taataaatta tcacttacat taatgctgta ttcttcatta acaaaccgac
    32101 tatcgaagtg aatttccatt agtgtaccct tacgattata aataaatgtt ccgtatgaat
    32161 aatctttatc gcttgtataa tctctgaatt caattttaaa cttacgggag ttcgtaaaat
    32221 atttgtattt ttgttgttgc tcaactgtaa acgatttaat agtcattaca tggtcagaga
    32281 cggatttttt aatggtgtaa ccgtgcggat cttcatattc ttcgtcccca acccacacgc
    32341 cgtcagtcag tgtatatttt tgttcaaagt ctacgccatt atgttttccg gttaaggtaa
    32401 cttttgaatg ttctggcgtt ttaagttttt ctgtaatccc gaccatccga tcaaaatcaa
    32461 cagggatgta caggatcaaa ttatctaatg atcctctaaa ccaaaacaat acgcaacgcg
    32521 gattaaaaaa atgattgttt atatgaatga acatttccgt gatcctaatt taaccattgc
    32581 caaaatagcg gagtcttctg gttattcttt aagtcacttt agtaaagcat ttaaactata
    32641 catggggcat tcgcccattt cgcatcttaa tcgtttacgg ctttcagaag cacgctattt
    32701 attacataca acacatttac catttaaaga aattgcgctc caatgtggct ttaaaaatat
    32761 ttctaccttt acggaaagtt ttaaacaaag tataggaata ttgcctaaag attatcgcaa
    32821 aaagtatcaa acaccaaata gttaatagaa aataaaaata attaagttag catttaattg
    32881 atttatagcg ataaactcgt gataaatagg tggtttttat atgtgcgttt tgtttttata
    32941 ataacgctta catttttggg gtttattatt tataataata gagtcattat aaacggaggt
    33001 caaaatatgg ctattaagaa aaaatttgtc tccctagatg gaaacgccgc agccgcagag
    33061 gtaagttatc tctacacgga tatcgcggcg atttatccga ttaccccatc ttcaccgatg
    33121 gcggaactcg ttgatttatt cgcatcacaa gggaaattaa actactttgg cagcaaagta
    33181 aaagtaacag aacttcaaag tgaagctggc gctgctggtg cggtgcacgg tgctttacaa
    33241 gccggaacct tagcgacaac atataccgcg tcacaaggtt tacttttaat ggtgccaaat
    33301 atttacaaat gggttggtga acttttacca gcagtactgc atgttagtgc ccgtagtatt
    33361 gccgcgcgtt ccttatccat ttttggtgat catcaagata tttatgctgt acggcaaact
    33421 ggtgtgacga tgattgcaag tcacagtgtc caagaagtcg ctgatttagc gcacgcagca
    33481 cacttaacag caattggatc aagtgctcca gtcttacact tctttgatgg ttttagaaca
    33541 tcacatgaaa ttcaaaaagt tgaagtcatt gatgaaaatg aatggaagaa attattacca
    33601 gaagataaag tagcggaatt ccgcgctcgt gcactgaacc cacacgcaaa tcctgtgacc
    33661 cgtggtggtg cagaaaatga tgatatttat ttccaaggta aagaagcaca aaatacacat
    33721 tacgctcgtg tgcttgatgt tgcttgtgat tattttgcta aagttagtaa agtaaccggt
    33781 cgtcactatg caccgtttgt ctatctcgga gccaaagacg ctacaaaagt aatcgtcgcg
    33841 atgggcagtg ttactgaaac aatccgtgaa gtcgttgccg atttaaataa aaaaggcgaa
    33901 aaagttggcc ttgttaaagt gcacttatat cgtccgttta gtgccaaaca tttagtcgat
    33961 gttttaccag aaacagtgaa gaaaatcgct gtcttagatc gcactaaaga agtaggagca
    34021 cttggtgaac cactttatct tgatgtgtta accgcactta aacaacaagg acggaaagtt
    34081 gatgttatcg ttggtggtcg ctacggaatt agtagccgtg acacacaacc aaaacatatt
    34141 aaaggagtat ttgacttctt agataataaa ccgtggcatt catttaccgt tggaattaat
    34201 gatgatgtca ctaaacttag tgtcccattt gatcacgatt atcatattga taacggacat
    34261 acggaatgct tattctacgg tttaggaagt gacggcactg tgagcgctaa taagtcaagc
    34321 gtcgccatta ttggtgaaca cactgataaa tatgcgcaag cttactttgc ctatgactca
    34381 cgtaaagctg gtggtccaac ccgcagtcac ttacggtttg gcacaaatac gattcattca
    34441 acttattacg tagaaaatgc tagctttatt agtgcttcac aagattctta tctttataaa
    34501 tttgatatga ttcgctactt aaaagcaggc ggaacattct tacttaacac aagcttaaaa
    34561 ggtgaagaat taattaatgc attacctaac agagttaaat acggtttagc aatgaaaaat
    34621 gccaagatgt atgtaattga cgcaaataag attgctagtg aaatcggtat gggtcgccat
    34681 acaaacacaa tcttacaatc ttcattcttc tatctaaatc aagatattat gccttacgaa
    34741 aaagcgttgc gcttaatgaa aaaatacgct gaagcggctt atggtcgtcg cgggaaacgc
    34801 attgtcgaac ttaactatgc ggcaatcgat gctggctcac acggtatcat tgaagttaaa
    34861 gttgatccag aatggaagaa acttaaacca ctacgcactg tggaacttat gggtgataag
    34921 tattttgatg aatatgttaa cccaattgat aacttagaag gttacgactt accagtttca
    34981 acattcacaa agtgggactt acttgatggc acgatgacta atgacatcac cttcaaacaa
    35041 aaacgctcaa tcgcgacaat ggtgccacgc tgggttaaag aaaactgtat ccaatgtaat
    35101 acatgtagtc tagtgtgtcc gcacgccaca attcgtccgt tcttacttga cgaaaaagaa
    35161 gtggcaggct taccagaaaa tgtgcgtgaa gaaacagtcg cggcaatggg tggaccaagc
    35221 gtagcaggct tacgcttccg tattcaagtt agtccaatga actgtgtcgg ctgtgccctt
    35281 tgtgttgtca cttgtccagg acgcggcggg aataaagcac ttgaaatggt tgaagcacgt
    35341 agtcaatttg acattgaacc tgctgccgac tacttatata agcaagttac ttataaaacc
    35401 gacaaattcc cacttaattc accaaaaggt gttggtttct taatgcctta tatggaaatt
    35461 agcggagcat gtgcgggctg tggtgaaaca ccttactacc gcttaattac gcaattattc
    35521 ggtaatgaca tgctagtagc aaatgcgact ggctgtagct cgatttattg tggtagtgtc
    35581 ccaggtacac catttaccac gaacaaagac ggtgaaggcg tcgcttgggc gaatagtctg
    35641 ttcgaagata acgcagaata cggttttgga atgcggatgg ctaacgatta taaacttaaa
    35701 ttaatcgctg acatcttcac tgcggcttta ccaaaagttg aaccagaact aaaagaactc
    35761 cttgaaaaat atctcgcaaa tccgcgtgac cggaaagaag ttagaagcta cttaccacgc
    35821 ttaattgaat tacttgaaaa aagtaaagac aaaggcgtta aagaagcact taaatataaa
    35881 gatgacattc ttgataaatc agtgtggatt attggtggag atggctgggc atatgatatt
    35941 ggttacggcg gtcttgatca tgtccttgct agtggcgcaa atgttaatgt gcttgtgtta
    36001 gacactgaag tgtatagtaa caccggcgga caaatgagta aagcaacgca aactggtgct
    36061 attgctaaat ttgccgcaag tggtaaaaca ggggcaaaga aaaacttagc aatgatggcg
    36121 atggcttatg gggacgttta tgtcgcccaa gtcgcacttg gcgctaaccc agttgccacg
    36181 atccgtgcgc ttactgaagc ggaaaattac gacggaccat caattgttat cgcttatagt
    36241 ccgtgtactg agcacgggat taaaggcgga cttagtaacc atatcgccgt tgaaaagaaa
    36301 gccgtcgcta gtggttactg gccaatcttc cgttatgacc cacggcttga agcagcgggt
    36361 aaatcaccac ttacttggga tatgaaagaa ccagattaca cacaattccg tgaatttgtg
    36421 atgggtgaaa cacggtttag tcaactccca actgtgaacc cagaaaacgc tgaattctta
    36481 cttagtaaga gtgaagaaga agcgaaaaaa cgctacttcc gtcttaaaaa gtttgaataa
    36541 cgctctttaa attaaaagag agcctcggga cgaccccgag gcttttcatt ttggacctca
    36601 ttttggacct catttttaaa tattatatac gacaatatat acgatatctg tataatttcg
    36661 attataaagt gtaacgataa caccattatc agaaattaaa aatattcgtt tttttacttt
    36721 gtactctatg tttaaaaata taaataatta gctatttatt atctaaaaaa tattgaatat
    36781 taatttctaa gtcttcccac ggtacacaag cgcgcatatt tttagcaata gtggaaatat
    36841 gagttccggc ttctttaccc gcaacagcag cgtcataggc tgctagtatt tttgcttcag
    36901 gggcagcaca tgcttcataa atccaaagta ataattctgg acataatagt ttagtgtaca
    36961 cgcggttttt tgctgaatca gtccaagtta taccgccttc ttctagtgta cgatagagcc
    37021 atgaatcaaa gaagtgccac ttttgatcag gttcacagat tgatttatcg acgactttac
    37081 caggttgacg cgcgtaatct atataaccac cagttttttg ttgatattct ttgataaacg
    37141 cgtcagaaat tgggtaatga gcgtctttag tttgtaaaaa ttcagcaata tcacgcattg
    37201 taatatagtc ataagttccg tctacactag tcgggatttc gcttgtagta ctagtctctt
    37261 cactactact tattggttca gaagaagtag gtgtttcact gctaggtgga aggccgcctt
    37321 cctccactag tgcgtaaaat cttatattat caaacccgca gtccatcgta cttgagatta
    37381 atgtttccgt atagaaagta aacactagct cagtaagttc tgtggtaaga atttcaacat
    37441 cacgtaagat atcttcgttt ttagttcctt gataaatagc gcctgtattt gttttaatgc
    37501 gggtgtgggt tcgttcttgt tttaaatcca aaacaatttt agtgtagccg cttaccttaa
    37561 ttgtgcaaat acgtggttct tccgtggtag aagcaggcat ccgcatccct agtgatttat
    37621 tatcgatact tgtttttttc gtcgttgtta ccctgccgtc agccacaatt aaaccgtcgt
    37681 aataagtatt atgatgtgtg gcactcatat taccagcatc taattcttca aaatcaatcg
    37741 tgcgcactag tgtttccaca agtggagcgg tggacgcaga aggtatttct gctgaagatt
    37801 ca
//
LOCUS       DAOSLP010000007        30342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_1710_length_30342_cov_6.427642, whole genome shotgun sequence.
ACCESSION   DAOSLP010000007 DAOSLP010000000
VERSION     DAOSLP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 30342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30342
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1710_length_30342_cov_6.427642"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1991
                     /locus_tag="PK460_01375"
     CDS             <1..1991
                     /locus_tag="PK460_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOE53785.1"
                     /translation="LIVGLSVAKEKTPVGVKAAPVYTITEDFETQTALTASYADGNFV
                     GAGGITFYYGHSRDQDKYPIDGKGIMLRRASDSYLEFQLKKGLSGLKFKYRKGFTGPG
                     ARQLELLVNNTQVSLTAEFGADSGALATIFDFVYEPDVSHSGLINVKIKVPGTADSNK
                     HVILDNIQIAENSASTDVITALSVTPTSKTYYTTDTLQETDFAVNLTKNGATAPGSSA
                     NYDYFVKIGTGTGTTFVGEYISWGVTQPLTTHTNIQFKACLPQTAGNTDFIIVDIPIT
                     VSVPTLSSIAITGNMTKTSYFVGGVWNPAGLTVTATYTNSAVVDVTNEVVWTYNPATP
                     NSTTITSVAVTATYNTMTAVHNENVTVTERPVSVVISEVYGGGGNSGATYVYDYVELY
                     NLTNADIDLSTYSLWYGSSTGGTLGQRTPLSGTILANGYFLIKFKTNDATVGAVSKVL
                     EDIDASVTGFNIAGSNFKVAISNSETAPTGADDTSVVDFVGAGTANDYEGSGTAPAPN
                     ATTSISRKLTAAGIPQDTNDNAADFELTTGGFSPMNSALGVANFIMAENTEGQCTTRF
                     ALAKAQLLKLSAAQLEYFKTGAQDETATLLTDARARYVAWSVYLGESNIYGAGGGSPR
                     IDINTEKDNTISVAVAIGVIGLSALLGFYFLQKKKKLA"
     gene            2217..3563
                     /locus_tag="PK460_01380"
     CDS             2217..3563
                     /locus_tag="PK460_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE53786.1"
                     /translation="MLLEFSISNFMSFEEKNTLSMIPSIQSNRGTFNISGINVLKFAT
                     IYGGNASGKSNLIKALSTLTYIVRLGLDERHRKLYCRVHEDNKDKPTIFSVTFYANEK
                     FYNYYFEVLLAERKVIFESLSELNPKTGNGKEIYTYTKGELDYKKVFNLKNIDEFNKN
                     RLKTYLFDNNMTQHKLLITDLISKEWDDSQLSFIKDVIHWFSHININMADFPIHDMKY
                     LEKVDERNKVEDILRELNTGVSKIDFTKISEDEFASRTNPHFAKAIIKDVKERYSEIV
                     KSGKKNIREFTLRGDSYYRFLFKPQEENIDILELSLKHENCDTKFDFGDESDGTQRLF
                     DLLDIVFTSQPNSVYVVDELERSLHPVLTYKLIEILKEELQKKNIQLIFTTHETHIMD
                     LKLFRKDEIWFMERDNKGLSRLYSLDIFKIRNEKIIKDGYINGLYGGIPLFKKLEE"
     gene            3687..4274
                     /locus_tag="PK460_01385"
                     /pseudo
     CDS             3687..4274
                     /locus_tag="PK460_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
     gene            4349..5149
                     /locus_tag="PK460_01390"
     CDS             4349..5149
                     /locus_tag="PK460_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53787.1"
                     /translation="MIKRRPEFDPFRDKKVFRCKFCNFPITNPGLYPGKKIMINRYCP
                     ACHSNNTFALLNKQDQTFLRMGFFSNPLYPISPHHLKGVPLKFSLIVFPLSVFFNFVA
                     NIVYMPFSLIYNNIQIKKALKEFYASYKDQDTNLILIDMLEKKAGPKNQYASYKTGIA
                     YLEGIHVPYDLEKAESYFKIAKEEHEGGLVKLAETLIRSENPNYDKAFTILNLVKDNP
                     LASYYLAYLYFYGYGVTPSLRIATIYAGTAHTHNIKDYYDIYPKILQI"
     gene            5304..5465
                     /locus_tag="PK460_01395"
     CDS             5304..5465
                     /locus_tag="PK460_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53788.1"
                     /translation="MEKLVFETIESLLEIKKLRIEERGPVLSRLTDDEIVLLIDNLVE
                     DLQSEYLVQ"
     gene            complement(5512..6390)
                     /locus_tag="PK460_01400"
     CDS             complement(5512..6390)
                     /locus_tag="PK460_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE53789.1"
                     /translation="MIDDKLKTLIILAKVKNYTQTAELCNLSQPAVTQHIKALESRYG
                     IEIFRRNGRNLTLTYEGKSLVDSAKKLIALERNIQKEIKKQKGQPKKLDIGITLTAGG
                     YFIPEIMEVFKDKYPNLRFNFHTDIAENILERMRLNELDFAIIDGTSQTDEFNIELLF
                     RDELIIIGPKDHRLADKKNVSIEELKKEKFILRHEKANTRLAFENYLSNHFDNINNFD
                     VILEIDNTAMIKQLIIAGHGLSVMSKAICEINMQVGTLKQIDLRDFHLERGIYLVYPK
                     ELENSEIIDSILALKE"
     gene            6512..7879
                     /locus_tag="PK460_01405"
     CDS             6512..7879
                     /locus_tag="PK460_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE53790.1"
                     /translation="MVPFLLSSPFKDTSVPTVLMALSIMLLVGFLLTRLTKPLKLPNV
                     TAYIFAGIILGPLAQLILGGKPLFPDELRKGMEFITDLALAFIAFGVVRYFKFDILKK
                     SGPKILIITLFESLMAVAFSFGAMMLMNLIPGVKMDWELAILLAAIASATAPASTMMT
                     IRQTGAKGDFVNTILQVVSLDNAIALILFSVAMAIITGNKESGGINFMQLAKPILLNL
                     ALIVAGYLMGLLLYWVTPPKRTKDNRLIIAVAFMLGFSGVASLLDISPLLGVMAMGMA
                     YTNIAPDESLFRQIDAFSPPILTIFFVLSGMRLQLHMLASIGVVGVVYFIVRIIGKYT
                     GATLGSIVARSTENNKKYLGLALIPQAGVSLGLAALGSRILMGYGHNDYADMLTTIIV
                     AAGVLYEIFGPPAAKYSLSITKSYTTEKTVEMIGPKPSEIPVSAEPKEVPVEESIAEI
                     KAETK"
     gene            7928..8845
                     /locus_tag="PK460_01410"
     CDS             7928..8845
                     /locus_tag="PK460_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53791.1"
                     /translation="MMDKLIDYFEHGEIDKVIALSKGSKDPEIQFFYLAALRYLGEYQ
                     IALSFISENQMQLYTNNAPQLIDWHIDILLELDDLDQALNTLKIYETFPYFSLETNEL
                     IAKLGDKVQQKRKEKNRQHKFDLFELERRLLCRNVELAFSAVSYMVSNFHEAYIALFK
                     RALLDAPINNVKSIIIFALKELKHNETVQVNKFGKLIKVNPATAPDPFKTKGGAKLIK
                     KMQEVAALDDYNQFSEVADSLITGHAMYIYPLTYEVKDVDGLGDAYLYYIYRALGRVN
                     NVNEYIEEHGLNAETLFAIFTKYHFTYFD"
     gene            8989..10263
                     /gene="tig"
                     /locus_tag="PK460_01415"
     CDS             8989..10263
                     /gene="tig"
                     /locus_tag="PK460_01415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOE53792.1"
                     /translation="MKLKVTKLETSKYELEVEVSEAKWSAAREKAFKKLAKDVTVQGF
                     RKGKAPEHLVRAKIDPVKELDEAVHSLLDEAFQFAIKESGHYPVIQPTYDVVKVSDTE
                     LVLKFIIVAAPVVTLGQYEGLKIGRGEANVEDSAVEVRLNSILEQNAELILKEDAAAL
                     GDTVVLDFEGFVDGEAFEGGKATNHELELGSGQFIPGFEDALVGVKAGESRDVNVTFP
                     ENYHEQLAGKKAVFKTTVHEVKEKRLPALTDDFAASLNMPNVTDVASLRVHVLFELTK
                     QAEAAERNEYVEKILAKLRETSTFELANDIIDREVEHMDERLKNQVTQQGMEWKQYLE
                     LTKQTEEEIKERFVSEATVNLQNFLVLNEIAKAKEIEVTDAEVDFEIAMMAQQYNMEE
                     KRIRELLGDNINNLRNDVRQKKIINFLLENNE"
     gene            10306..12654
                     /gene="lon"
                     /locus_tag="PK460_01420"
     CDS             10306..12654
                     /gene="lon"
                     /locus_tag="PK460_01420"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOE53793.1"
                     /translation="MANKNTAKRYELPLIITRNQVYFPFNQSDDLDAGRPATVEAIKY
                     ANNSDDKLLIVATQKVFTVNNPSMNDIYEVGVLARLTKVRERPHHITVQLEPLARVFI
                     NELDESGPFIIADATVTFHEPLDPDANPERLKNALFDFFNDKLELTRRLHIDLDALKA
                     ADMNLEELIYFLANKTLNSTEQRMAVLAENSLERRIDILLKFLEDDVANDAVGAITQI
                     VRNRPQSGEGLDEVERDGLPLEDDEDEDEDEEFDTGEEILAQLKKRYFPKNFVTRIKK
                     EAKKMGRVEPSERSRIIEYLDWLMKLPYDQETIDNLDLENVRKVLDKDHFGLEEPKKR
                     IVEFMAVKRLAKENRATVLCFYGPPGTGKTSLATSIATAMGRKLVKASLGGVDDESKL
                     RGFLRTYVGAQPGIIISSMRKAGTINPIFVLDEVDKLGKSNRGDPSSALLEILDPEQN
                     KGFIDHYIDEPYDLSKVVFIATANYYWDIPRPLRDRMEMIELKGYTEEEKVQIAEKHL
                     IPNGIESHGLKGYDIKFTDEALRFIIGGYTWEAGVRMLNQKISAIFRKLSVEVLSDVE
                     SNFTITPEEVQKHLGKPRNFFTKKEPEPIVGVVTGISVAGNIGDIMKIEVTTFKGKGV
                     VNVTGNLEKMLKESGEIAASHVRSYAEKYGINPDVFLDTDINVHFPQASTKDGNSAGV
                     AMTIGIISVLTDRKINPNVAMTGEVTLTGRVLAIGGLYEKILGCIRAGIETVLFPEEN
                     VVDLDEIPQEVKDKVKLVPIKNIDEAVAIALLPKKVKKKATA"
     gene            12662..13255
                     /gene="yihA"
                     /locus_tag="PK460_01425"
     CDS             12662..13255
                     /gene="yihA"
                     /locus_tag="PK460_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOE53794.1"
                     /translation="MINFQNTLFVGSFTNKNEGAIEALPEIIFIGRSNVGKSSLINAI
                     INRKNLAYTSKKPGFTKLLNYYNVDDTFYLVDAPGYGFAKREKDLSASFATMMNNYFN
                     KNTNLSMIYLLIDSRRTLTADDEAFLDFVSHHKFPLTLIFTKSDKLNQSMRAKALNFY
                     KKTFKGEVKAIFTSSHNKENISKVHEHISDTLKNLKA"
     gene            13517..16102
                     /locus_tag="PK460_01430"
     CDS             13517..16102
                     /locus_tag="PK460_01430"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOE53795.1"
                     /translation="MKLDTHYNHLKVEKGRYEHWKKSGYFKSGDTSKPKFSVVIPPPN
                     VTGVLHLGHAWDNTFQDIIARYKKAQGFDVLYLPGMDHAGIATQARIDGRLRERGLSR
                     YDLGREKFLEEAWKWKEEYASTIRSQWAKLGLMLDYDHERFTLDKGLNDAVNHTFVEL
                     YNRGYLYLGERIINWDPVFQTALSNIEVIHKDVEGSFYYFKYKLEGSDFYLSVATTRP
                     ETMFADVALVINPRDERYTKYLGKNFVNPVNNELIPLIADDYVDLEFGTGVMKCTPAH
                     DVNDFKIGERHNLSRPICLNKDATLNERGLELAGLDRFDAREKLVAKMKELDLLIKVE
                     KIVHNVGHSERSDAIIEPYLSKQWFLRMDHFAKKSIEGQKGDDPIRFFPKRFNKTYLN
                     WMEIAEDWCVSRQIWWGHRIPAYYHKETGEIVVSVTPPKDLENYRQDEDVLDTWFSSA
                     LWPFSTLDWPNTDSELFKRYYPTDVMVTGYDIIFFWVSRMIFMGLELTNEKPFKDVVI
                     HGLIRDEQGRKMSKSLGNGVDPIEVIAKYGVDSLRYFLAGASTPGQDARYSEARIQAS
                     VNYLNKIWNAARFLLLNLPEDFKPGKINHKKLHPVDAFILSRYYETVRKVTSLMDKYE
                     LGMASNYLYAFVYDDYCSNYIEMVKVILNSDGDHTNTYNVMYHTLHGILMMIYPYSPF
                     ISEEIYLALPNHLDSIMLASYPKASRLRFKREAEEVNTIINLIKEIRNFKSTNNLAPN
                     TKMNLYVADVSFDLTPYLDIIRRLGFINEFKYSDVPIEVTTVSLPKATFGLELDVDRS
                     VLISQLEKEIETLTKEVERSTKLLNNPGFKNKAPASKVAEEEEKARHYAESLALAVDK
                     LTKLR"
     gene            16145..16321
                     /locus_tag="PK460_01435"
     CDS             16145..16321
                     /locus_tag="PK460_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53796.1"
                     /translation="MTLLTNAEMANIKGGEPITLAAVMTILVIAIVTVIVYKLFTSNA
                     GSTTIPGGFKFEWK"
     gene            16308..16994
                     /gene="radC"
                     /locus_tag="PK460_01440"
     CDS             16308..16994
                     /gene="radC"
                     /locus_tag="PK460_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOE53797.1"
                     /translation="MNGSKRIKELPHLKRPREKALYYGIGSLSDAEVLALLIGKGTTK
                     DNALSLAKKLLNKHISLQNMYRIDDPKLLLMPGIGEVKALQILAAFELNKRLLFLEGS
                     HDRPIYREDEIVRSFQRRIGRLQKETLYLLALTNRNYIISEKQLYLGTEKGFSLDPKE
                     VVRELLIANADRYILIHNHPSGHVEPSANDLETTIELATISAQFGVTLYDHLIIGANA
                     YFSLRKLGKL"
     gene            complement(17056..17892)
                     /locus_tag="PK460_01445"
     CDS             complement(17056..17892)
                     /locus_tag="PK460_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE53798.1"
                     /translation="MINITSIKAKTRNLASQIGLSANIILRMYFFERFLMRLSKSLYK
                     DKFIIKGGFLISSIIGITNRTTIDIDTNIKDMNLEQEMITNVIKEIINIDINDGVEFI
                     FTNIEIIREIDYYDNFRISLVAMLENTRIPLKLDITTGDPITPSKIVYEYSCLLTSEK
                     IRLYAYNIETIIAEKYETIIVRNIETTRMKDFYDVYMIFKLKHNAINFKTLKRAIFNT
                     SNKRKTTKIIKDSKEILNNMQNNSKLINEWEKFMQKNSYAADIKFFDTIEILKTIADI
                     IV"
     gene            complement(17889..18476)
                     /locus_tag="PK460_01450"
     CDS             complement(17889..18476)
                     /locus_tag="PK460_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOE53799.1"
                     /translation="MTSLQNYLNERPVITNKDAARIGVNRQLLSVLVKEGKLERLRPG
                     VYQKAGEVIDDFMLVSANSNQVCFSHETALILHDLSDRMPDRLYITVPQGYNTSHIKK
                     RYINLKTRYVKRKHHKIGIITMKTYFGNKILVYDMERTICDIIADRENIEKEVFVDAI
                     TRYFRRKDKDIKKLIKYSRQFKIENEILRYIEILS"
     gene            18829..19137
                     /gene="rplU"
                     /locus_tag="PK460_01455"
     CDS             18829..19137
                     /gene="rplU"
                     /locus_tag="PK460_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOE53800.1"
                     /translation="MYAIIETGGKQVKVATGDVIYVEKLDVAEGEDFVFDKVLLVGGK
                     KAKVGTPYVKGATVSAKVVKHGLNRKIKVFTYKNKSNVRRMLGHRQPYTCLEVVEIKG
                     "
     gene            19140..19463
                     /locus_tag="PK460_01460"
     CDS             19140..19463
                     /locus_tag="PK460_01460"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOE53801.1"
                     /translation="MINVVITTIDEEILKIRIKGHAYSGEPGHDLVCAGVSAVSIGAL
                     NSLHEVDTAFKVTVEDGLVEIVPLYRPSYENKIVLQVLITSLKTIAESYGKYVQIREE
                     RRAEA"
     gene            19460..19744
                     /gene="rpmA"
                     /locus_tag="PK460_01465"
     CDS             19460..19744
                     /gene="rpmA"
                     /locus_tag="PK460_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOE53802.1"
                     /translation="MKFILNLQLFASKKGVGSTRNGRDSTGRRLGAKRADGQLAKAGA
                     ILYRQRGTRIYPGLNARRGSDDTIYATVEGIVRFERFGKTRKRVSVYPVN"
     gene            19833..20795
                     /locus_tag="PK460_01470"
     CDS             19833..20795
                     /locus_tag="PK460_01470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE53803.1"
                     /translation="MAKKLVTVVVPMYNEEPMVPHFLTKIAEIIKENDGYNFEVVAVN
                     DGSRDNTLALLYDFQKKYKFLRVVNLSRNFGHEAAVAAGLSVAKGDAIIPIDADLQDP
                     PELIKPMLELFEEGYDVVNARRASRKEDTWIKRYTAGKFYKVIDKLSGKVRIPQNVGH
                     YRLISRRVLDEVLKLPESNRVFRVEVPYVGFKTAEVAFVRPKREHGKTHYNMKSMIDL
                     AINAITITSTKPLNFVFKFAMWLGIIFGLSAVTQLVLFILEMTKVLVLPFLNHGVWLL
                     INVSLFLTTVILLALTIISIYLGKTFNETQKRPFYVIESVKENK"
     gene            20799..22082
                     /gene="obgE"
                     /locus_tag="PK460_01475"
     CDS             20799..22082
                     /gene="obgE"
                     /locus_tag="PK460_01475"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOE53804.1"
                     /translation="MFIDQAKITLKAGNGGNGMVSFRREKYVPKGGPDGGNGGRGGSI
                     YFVATSNISTLTDFRFGLTIKANNGGNGATKNQYGKSGEDLYVKVPIGTVVYLEPSRE
                     VIADFKLEGETMLIAKGGKGGRGNAAFKSSTNRVPRIAEHGVPGEELLVTLELKLLAD
                     IGFIGLPNAGKSTLLTVISEARPQIDNYPFTTLRPELGVVKANSGNRFVAADLPGIIK
                     DAHLGRGLGLRFLRHAQRCKVLAFIIDGENSEDYYKEYKLLRNEVKEYGYKLDERPFF
                     VAVSKDETVDTHKKVKALAKKLKDIKVLGFSSLLHENIDTLINTMEEYIVNAPEIEMV
                     DEEGVAVKYREYDLTATIDPDLFTIEKVRAGVYRIVGEKVEERYHKYILNTEEAILSL
                     LNYLREIGVEDALDKMGVNDGDTVILVDFEFEYFR"
     gene            22092..22820
                     /gene="nadE"
                     /locus_tag="PK460_01480"
     CDS             22092..22820
                     /gene="nadE"
                     /locus_tag="PK460_01480"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOE53805.1"
                     /translation="MGQESLQNYLQYLVRYLKRRARGFNGYILGLSGGLDSAVVALLA
                     QKAVKDDLLCVIIDIESEKCDMDDALELCKLHNLNYYYIDLTAEYRAVVKKLEESKKL
                     TELSKINTKVRLRMVTLYALGQTHSKLVLGTDNKAELFTGYFTKHGDGAADLFVLSSL
                     TKGEVFAAARLLGVTPNIINKIPSAGLYVGQTDEAEMGIKYEDLDAFISGKEVSPSVK
                     ARAIELHRISNHKRRPIPRPREKR"
     gene            22918..23475
                     /gene="ruvA"
                     /locus_tag="PK460_01485"
     CDS             22918..23475
                     /gene="ruvA"
                     /locus_tag="PK460_01485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOE53806.1"
                     /translation="MIYGLKGIVTKQTATTIVLDVRGVYYEVKVPSVPQQQQFSELFL
                     YTYQVIREDEHYLVGFLSLEEKSLFTELLNVSGIGPKTALGIMTATTPERFLQAVTSE
                     ELSYLKKLPGVGPKGASQIILDLKGKLQTDTSKGLNPYEQEVTLGLQSLGFKKSEITK
                     VLKKVSNKSLDANELLRLALSELRR"
     gene            23475..24482
                     /gene="ruvB"
                     /locus_tag="PK460_01490"
     CDS             23475..24482
                     /gene="ruvB"
                     /locus_tag="PK460_01490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOE53807.1"
                     /translation="MSNRLVSDKLDQSEASVELSLRPLTLNEYVGQTALKHNLNIFIT
                     AAKARNEVLDHTLLFGPPGLGKTTLAHIIANEMGANIKVINGPSIEKAGDLAAILTTL
                     EAGDVLFIDEIHRLPRVVEEVLYSAMEDFKLQVMITLDGNVKNLQVDLPPFTLVGATT
                     RAGDLSSPLRARFGISEKIDYYEESDIFNIIKRTSRVFELPINDDAALEIARRSRRTP
                     RIANRLFRRIRDFATFASKRVIEYELCLEALKHLRVDTLGLDEVDIAYLTTLVERFRG
                     GPVGLETLANAIGEEVTNLEDVYEPYLLQIGMIDKTPRGRVATEICYAHLKKPRQTHL
                     F"
     gene            24602..27211
                     /locus_tag="PK460_01495"
     CDS             24602..27211
                     /locus_tag="PK460_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53808.1"
                     /translation="MRRLTAYLTLTFTLIFGAALNMFSPLRNLVSNHEYQTGREFVYR
                     ISDKDPDKDLDPKAIDEIVTIMDRRMQNYGVSEYNIAKEGKNIVRVSTTLQSTEEYNR
                     LQVYLNYNANFTMKIGDDDNTEAILSADQIFADKKARVEYRGPYPFIVFPLSDPTLFE
                     TTIVDVAQKIQEEKGSQETAEGEDKPALVKEATIVLWSDYDPDKDSYMSPVGDTQGKI
                     FLSFDYRSMWWNEDKTEIGVASPLEGMSENHKYTTAQIKKASETARYMMNVFNAGTLD
                     YKVDFLYANKAMAPTVEPLINLGTRDNIALSATFFASLVTMVVVLVILFAIYRLPVLN
                     ALSSSLLTFLGTMIIFNAIQVEFSTSAIIGVLIVTFVSILSTILYAAKFRNEVYKGRT
                     FKKAHTEAITRSSFLTIDILVLLLVTGIFTYFFGGAALTGFATTLIFGSIVMVASVLI
                     HNSLLLPLLAHNRVTSKSYTYYGINESKVPDTLKDEKQTYFGRFVNHKPENRAKLSMI
                     LTGSLSLITAVLLIVFSAVNINPYNLVKHDENITRVYFQVSENSDINPLSPTASPVTL
                     LNYIELDGEALTFEKKEGAPVYETQVYKTMELDIEDEIEVTYNFYVYNLIGVYTGEEE
                     VNYKVTADAPMQSATLSEALDIIVKNIDDKAVISFNAVEKEAITPHFGRIAWVSLVIL
                     AVTSFYLTIRFGLTRGITNFVESFVAASLVFLFLMVTRLAIPPLATMGAFVAILAVSF
                     LNVVIFTNIKDVQEETASKGLTHREHFELGLRYSLSVIYITVLMFSLGFLVFTLIGPL
                     AVSSVYLASAVAMVAALLVVVKYSTAFLSGSLNVLDRLPKLERKQKTVTGKQRTSIRS
                     NEPEEAIFIGIND"
     gene            27262..28812
                     /locus_tag="PK460_01500"
     CDS             27262..28812
                     /locus_tag="PK460_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOE53809.1"
                     /translation="MNKFLDKLLKYYDLSLRDYEKLTAEVTKENLPTAEAFFNFESFI
                     SRIEKAIASDENVVIYGDYDADGILATSILKYAFLKRGKDVFTMIPSRYKDGYGLNTN
                     VVEKFSKRAINLIITVDNGINQHDAIDLANELGIDVLVSDHHELSETLPNAIAILHPE
                     LSNLVRLNSCGAYMALVISYGMLGYYDDYLISLAAIATLADMMPLIDRNRTLVRLAIK
                     ATNDHQYFPLVKLNDSTFFDETSMSMKIAPKINALGRLSQNYESNVLVEYFTTTDVKR
                     HYDISDYIEKVYKKRKAMSRGEELSSEELKNPVIALITDELEGVLGLIAQNYMLEHNK
                     PAFIFTESSEDPSLLKGSVRSKSGFNVVDAFSHLEHLILTSGGHKEAGGLTIRKQDFI
                     VFKEALNKYAKNYPLITQDEKYLDVTLDEITKENYALIKNLSPFGHKFRAPLLRLTSL
                     TNVKYLFSRDGKHIITPLESGVKLIGFSLAEESKNALNIGFTATFAESEFRGRRSIDL
                     VIKEFFTA"
     gene            28899..>30342
                     /locus_tag="PK460_01505"
     CDS             28899..>30342
                     /locus_tag="PK460_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOE53810.1"
                     /translation="MKRRKLLLLPVFLLLLAGCKDTSSSVSVEDSQPTVTSTSEVEPS
                     SEEPQVTSVEDTEAPPSIPTSEAPVFEHKTIEEIRAMTEDGASATPDQKQLFYETSGV
                     VTAKFRGANISGVGPAWNIAIQDGEHALLLYAVTEEAYAADFADVPIGSKLTVTGNLA
                     AYKGLRELSTIKYVSHEAASPVEPEVLASIEQADLAGKDSKLVKIEGLKLHAKPQLEA
                     YVDGDRNRVNINMDLRKDGVKIGTYMHYNIPLADAQATVEKLQTVTSNSTVTWTGILG
                     MHEKVFQLTNANADEWEIEVGAPVVLETIAFRDETKELAPGATYQTTLVSTPFDAVVG
                     EVTYASDAAAVATVDAEGVVTAVAVGTANITATASGKVATMVITVADAAEGVYDITVV
                     DSYTTPSEIKTEEAFLAAVKGYPVGMTVPEVVKVYGIAPTMGSNQHNDYTATTGFKFS
                     SSKDNAALTLKMPAGVEIAKVKVEGRAWGKD"
BASE COUNT         9970 a         5022 c         5943 g         9407 t
ORIGIN      
        1 cactaattgt cggtcttagt gtggcaaaag aaaagacacc tgtaggggtg aaagctgctc
       61 cagtttacac cattactgag gactttgaaa cgcaaacagc tttaacggca tcatatgcgg
      121 atggaaattt tgttggggca ggtggaatta ctttctacta tgggcattcg cgagatcagg
      181 ataaatatcc tattgatggg aagggaatta tgctacgacg agcaagtgat tcttacctag
      241 aattccaatt aaaaaaagga ctctctggtc ttaaattcaa atatagaaaa ggatttactg
      301 gtccaggcgc tcgccaatta gaattgcttg ttaataatac acaggtttct cttactgcag
      361 agtttggtgc tgattcaggt gctcttgcaa ctatttttga ttttgtctat gaaccagatg
      421 tatcacactc tggtctaatt aatgtgaaaa ttaaagttcc tggtaccgca gattcaaaca
      481 aacatgttat acttgataat attcaaattg cagagaattc tgcatctaca gatgttatta
      541 cagcattaag cgtaacacca acatcaaaaa catattatac aactgatacc ttacaagaaa
      601 ctgattttgc agtgaatctt actaaaaatg gtgctactgc cccaggaagt agtgctaatt
      661 atgactattt tgtaaaaatt ggtactggta caggcacgac ttttgttggt gaatatattt
      721 cttggggagt tactcaaccg ttaacaaccc atacaaacat tcagtttaaa gcttgcttac
      781 cacaaactgc tggaaataca gactttatta tcgtagatat accaattaca gtttctgtac
      841 caacactatc ttcaattgcg attactggta acatgaccaa aacatcttat tttgttggtg
      901 gtgtttggaa tcccgctggt ttaactgtta ctgctacata tacaaactca gctgttgttg
      961 atgttacaaa tgaagtagtt tggacctata atccagcaac cccaaacagc acaactatta
     1021 ctagtgttgc tgttactgcg acatataata ctatgacagc agtacataac gaaaatgtca
     1081 ctgtcacaga aagaccagtt agcgtagtaa ttagcgaagt ttacggtggg ggcggtaata
     1141 gcggagccac ctatgtctat gactatgtag aactttataa cttaacaaat gctgatattg
     1201 atttatccac ttattcttta tggtatggat catctactgg aggcacctta ggtcaaagaa
     1261 ctccattatc tggtactatc ctagcaaacg gatatttctt aattaaattt aaaactaatg
     1321 atgccactgt tggcgcagtt agtaaagtcc ttgaagatat tgatgcttcc gttactggtt
     1381 ttaatattgc tggtagcaat ttcaaagttg cgatatcaaa ttctgaaact gctccaacag
     1441 gtgctgatga tacaagtgtt gtagactttg taggtgccgg aaccgctaat gattatgaag
     1501 gaagtggtac agcaccggct ccaaatgcaa caacatcaat ttcacgtaag ttaacagcgg
     1561 caggtatacc gcaagatact aatgataatg ctgcggactt tgaacttact acgggtggct
     1621 ttagtccaat gaatagtgcg cttggtgttg ctaactttat tatggcagaa aatactgaag
     1681 ggcaatgtac tacgcgcttt gcactagcaa aagcacaatt gcttaaatta agtgcagctc
     1741 aacttgaata ctttaaaact ggtgctcaag atgaaaccgc tacattatta acagatgcac
     1801 gtgcacgtta tgttgcttgg tcagtttacc ttggcgaatc aaatatttat ggtgctggcg
     1861 gtggtagccc ccgtattgat atcaatactg aaaaggataa cactatctct gtagcggtag
     1921 cgattggtgt tattggtcta agtgccttac ttggctttta tttcttacaa aagaaaaaga
     1981 aactagccta accctctact aactagagtt ttaagccgag actttgtgct cggcttttta
     2041 ttattactat gaatgagtac gaatgcagaa attaatattt ttaataaatt tacagataaa
     2101 atgtaaaaat atattgtatt tataattagt agataagaaa atataaagaa caattacaaa
     2161 aatattgtaa aagtgaaaag agcatggtaa tattaagaca gaaagagggt agtgatatgt
     2221 tattggaatt ttcgattagt aacttcatgt cttttgaaga aaaaaataca ttaagtatga
     2281 ttccaagtat tcaaagtaat cgtggtactt ttaatattag cggaattaat gtcttaaaat
     2341 ttgcgacaat ttatggtggt aatgcttcag gaaagagtaa tttaattaaa gcactatcaa
     2401 cactaactta cattgtacgg cttgggttag atgaaagaca tcggaaatta tattgtcgtg
     2461 tccatgaaga taataaagac aaaccaacta tttttagtgt tactttttac gctaatgaga
     2521 aattttataa ttattatttt gaagtccttt tagcggaacg aaaagtgatt tttgaatcgc
     2581 taagtgaatt aaaccctaaa actggtaacg gtaaagaaat ttatacatac accaaagggg
     2641 aattagatta taaaaaagta tttaatctta aaaatattga tgaatttaat aaaaatagac
     2701 ttaaaactta tttatttgat aataatatga cacaacataa attattaatt actgatttaa
     2761 ttagtaaaga atgggatgac agtcaattat cttttatcaa agatgttatt cattggttta
     2821 gccatattaa tattaatatg gctgattttc ccattcatga tatgaaatat ttagaaaaag
     2881 ttgatgaacg aaataaagta gaagatattt taagagaact aaatactggt gtgtcaaaaa
     2941 tcgattttac aaaaattagt gaagatgaat ttgcttcgcg aactaatcct cattttgcaa
     3001 aagcaatcat taaggatgtt aaggagcgat atagtgaaat tgtgaagagc ggtaaaaaga
     3061 atattcgcga atttactttg cgaggtgatt catattatcg gtttttattt aaaccacaag
     3121 aagaaaatat tgacatttta gaactaagtt taaaacatga aaactgcgac actaaatttg
     3181 atttcggtga tgaaagtgat ggtacacaaa gactttttga cttgctcgat attgtattta
     3241 cttcacaacc taacagtgtg tatgtcgtgg atgaacttga acgaagttta catccagttt
     3301 taacttataa attaattgaa atcttaaaag aagaactaca aaagaaaaat attcaactaa
     3361 ttttcacgac ccatgaaact catatcatgg atttaaaatt atttagaaaa gatgaaattt
     3421 ggtttatgga acgtgataat aaagggttat cgcgcttgta ttcattagat attttcaaaa
     3481 ttagaaatga aaaaataatt aaagacggat atattaatgg tttatatggg gggatcccct
     3541 tatttaagaa attagaggag taaaagatgc cgcctattct taaagctttt aattttaaaa
     3601 atagatatag cactaaaaga gtaggcacag ttgatacttt taaaacattt catattttat
     3661 gtgagtgaac agtaactgaa ccaaagtatt ttaaagcgat tcgcaattgc aactttttaa
     3721 atgtgcgcaa taatattgag gtcgaaattt taaaacgaac agagagtgat aaaggacgca
     3781 ctcaattaag cgagttattt aagttaatga agcaatatat tgatgataat aagttaacgg
     3841 atgacgatat acctgtttta gtcgttgatt ttgatgcgca caaagataat ccaaacttta
     3901 tcgatgaaat taaagcaatt gaagcatatg gtattaaggt atttggtaat tcaccatgct
     3961 tcgaattatg gctgatttta catattgaaa atgctttaaa tacctatatc ttaccgaaca
     4021 aaactgctat ctatcatgat gataaaataa gtaatgttca tactttcact agtagtttag
     4081 caagtaaaat attaggattt aacaccaaat atttaattaa gcccgaaatt ctaagcggaa
     4141 tatatcacgc tatcgatcaa agcacagaat tagagaacga tctttataaa ttaaataaag
     4201 atattggcac taatttgcag tttctaatta aagaaatatt acgtgatgaa cgttttacta
     4261 atgataataa ttagaaagat ttttatataa tttacataat aattaatgaa atttactata
     4321 atatagtaag tggaggtaag agtaacttat gattaaaaga agacctgaat ttgatccgtt
     4381 tcgtgataaa aaagtatttc gttgtaagtt ttgtaacttc ccaattacta accctggact
     4441 ttatcctggg aagaagatca tgattaatcg ctattgtcca gcgtgtcata gcaataacac
     4501 ttttgcgctt ttaaataaac aagatcaaac atttttacgg atgggtttct ttagtaaccc
     4561 gctttatcca attagcccac atcacttaaa aggcgtgcca ctgaagttta gtttaatcgt
     4621 gttcccctta agtgtgttct ttaactttgt cgcgaatatt gtgtatatgc cgtttagctt
     4681 aatttataac aatattcaaa ttaagaaggc actgaaagag ttttacgctt cttataaaga
     4741 ccaagacact aatttaatct taattgatat gttagagaag aaagctggtc caaagaatca
     4801 gtacgctagt tataagacag gtatagcgta cttagaaggc attcatgttc cttatgactt
     4861 agaaaaggcg gaatcttact ttaaaatagc aaaagaagaa catgaaggcg gcttagttaa
     4921 acttgcagaa acactgattc gcagtgaaaa cccaaattac gacaaagctt ttacgattct
     4981 taatttagta aaagataatc cgctagcgag ttattatctt gcttatcttt atttttatgg
     5041 ctacggtgta acgcctagcc tccgtattgc gacaatttac gctggtactg cgcacacgca
     5101 taatattaaa gattattacg acatttatcc aaaaatctta caaatctaaa ataaaataac
     5161 atacattcta tgtttgcttt actaattgta aagacttttt tacaaattta acactcctca
     5221 ttactttggt gagtaagtag tcttatgcta taatgcgttt gtagcaaaaa agcttatcta
     5281 gtggggagaa ggagtaaaag agtatggaaa aattagtgtt tgaaacaatt gaaagtttat
     5341 tagaaattaa aaagttacgg attgaagaac gtggtccagt attaagtaga ttaacggatg
     5401 atgaaatcgt cttattaatc gacaacttag tggaagactt acaaagtgaa tacttagtgc
     5461 aataataagt attcattttt ttagtgaagc acggtatatg gccgtgtttt tttattcttt
     5521 aagcgctaga atgctatcaa taatctcgct attttctaac tctttaggat aaacaaggta
     5581 aattccccgt tctaagtgga agtcgcgtaa atcgatttgt ttaagtgtac caacttgcat
     5641 atttatttca caaattgctt tactcattac acttaagccg tgaccggcaa taataagttg
     5701 cttaatcatt gccgtattat cgatttctaa aataacatca aagttattaa tattatcgaa
     5761 gtggttactt aaataatttt caaacgctag gcgtgtatta gctttttcgt gacgaagaat
     5821 aaacttttct ttttttaatt cttcaatact tacatttttc ttatcagcga gccgatgatc
     5881 tttaggtcca ataataatta actcatcacg gaagagcagt tcaatattaa attcatcagt
     5941 ctgtgatgtg ccgtcgatta tcgcaaaatc taattcattt aagcgcattc tctctaaaat
     6001 attttcggcg atatccgtat gaaaattaaa gcgtaaattt ggatatttat ctttaaatac
     6061 ctccatgatt tcgggaatga agtagccccc tgctgttaaa gtgatgccaa tatcaagttt
     6121 tttaggttga cctttttgct ttttaatttc tttttgaata ttacgttcaa gcgcaataag
     6181 ctttttagca ctatcgacaa ggcttttccc ttcataagtt aaagtaagat tacggccatt
     6241 acggcgaaat atctcgatgc cataacgact ttcaagtgct ttaatgtgct gcgttactgc
     6301 gggttgtgaa agattacaga gctcggcggt ctgtgtatag ttttttactt ttgctaagat
     6361 tataagtgtt tttaatttat catcaatcat cgtaaattta cctctgtttc taattataag
     6421 ttattcttat ataattataa caaattataa gttgttatat tagtaataag tgtgtataat
     6481 cataatacaa cactatataa ggaggcaagt tatggtcccg tttttacttt caagtccgtt
     6541 taaagacaca agtgtaccaa cagtactgat ggctttaagc attatgttac ttgttggctt
     6601 tcttttaacg cggctcacaa aacccctaaa gttaccaaac gtgacggctt atatttttgc
     6661 tggtattatt cttggaccac ttgcgcaact tattttaggt ggtaaaccct tattccccga
     6721 tgaactccgt aaaggaatgg agtttattac tgaccttgct ttagcgttta ttgcttttgg
     6781 tgttgtgcgt tactttaaat tcgacatatt aaagaaaagc ggaccaaaaa ttctcataat
     6841 tacattattt gagagcttaa tggctgttgc ttttagcttt ggagcgatga tgctgatgaa
     6901 cttaattccc ggagttaaaa tggactggga attagcgatt ttactcgcag cgattgctag
     6961 cgctacagca cctgcttcca cgatgatgac aattagacaa acaggcgcta aaggggactt
     7021 tgttaataca atcttacaag tagtgtcgct tgataacgcc attgctttaa ttctttttag
     7081 tgtggcgatg gcaattatta ctggtaataa agaaagtggg ggcattaact ttatgcaact
     7141 tgctaaacct attcttttaa acctcgcttt aattgtcgct gggtacttaa tgggtttact
     7201 tttatattgg gtaacaccgc caaaaagaac gaaagacaac cggcttatta tcgctgttgc
     7261 ctttatgctt ggctttagcg gggtcgcttc actcttagac atcagcccac ttttaggcgt
     7321 tatggcaatg gggatggctt acactaatat tgcaccagac gaatcacttt tccggcagat
     7381 tgatgccttt tcaccaccga ttctgacgat tttcttcgta ttaagtggga tgcggttaca
     7441 attacatatg cttgctagta tcggtgttgt aggtgttgtt tactttatcg tccgtattat
     7501 tggtaagtat acaggtgcga cgcttggtag tatcgtggcg cgtagcaccg agaataataa
     7561 gaagtactta ggtcttgctt taatcccgca agcgggtgtt agtctagggc tcgccgcgct
     7621 tggttcacgg attttaatgg gatatggcca taatgattac gctgatatgt tgacgactat
     7681 tatcgttgcc gccggtgtgc tttatgaaat ctttggacca ccagcagcga agtactcact
     7741 ctcaattacg aagtcttata cgaccgaaaa gacggtcgaa atgattggac caaaaccaag
     7801 cgaaatccct gtttcggctg aacctaaaga agtaccagtc gaagaaagta tcgccgaaat
     7861 taaagcggaa acaaaataat tatatttaat aacttaatag catatattat gctaatataa
     7921 ccttattatg atggataaat taattgacta ttttgaacac ggtgaaattg ataaagttat
     7981 cgcgctaagt aaagggagta aagatcctga aatccagttc ttttatttag cagcgctccg
     8041 ttatttaggg gagtaccaaa ttgctctatc atttatcagt gaaaatcaaa tgcaattata
     8101 cacaaataat gctccgcaac ttattgattg gcatattgat atattgcttg aattagatga
     8161 tttagatcaa gctttaaaca cacttaaaat ctatgaaact ttcccatact tcagtctaga
     8221 aacaaatgaa cttatcgcta aattaggcga taaagttcaa caaaaacgta aagaaaagaa
     8281 caggcaacat aaatttgacc tttttgaatt agaacgaaga ctattgtgcc gtaatgttga
     8341 acttgctttt agtgcggtta gttatatggt aagtaacttt catgaagctt atattgccct
     8401 atttaaaaga gcgctgcttg atgcaccaat taacaatgtt aaatcaataa taatttttgc
     8461 cttaaaagaa ttaaaacata atgaaactgt gcaagtgaat aagtttggga aattaattaa
     8521 agtaaacccc gcaacagcgc ctgatccttt taaaacgaaa ggcggggcta aattaattaa
     8581 aaaaatgcaa gaagtagcgg cgcttgatga ctataatcaa tttagtgaag tcgctgattc
     8641 tttaattaca ggtcatgcga tgtatattta cccgcttact tacgaagtaa aagatgttga
     8701 cggactaggg gacgcctacc tttattacat ttatcgtgcg ttagggcgtg ttaataatgt
     8761 taatgaatat attgaagaac acggtctaaa cgctgaaaca ctatttgcga tatttacaaa
     8821 gtatcacttt acctactttg attaatcatg tctttttatg atttaacgaa ataaaatgat
     8881 taaattgtcc ttaatcacgg aaatattaca taaataagcc actttattac taaaatgagc
     8941 gatattttag tatacttact aaggttataa atggaggaat ttttatatat gaaattaaaa
     9001 gtaactaaac ttgaaacaag taaatacgaa ttagaagtcg aagttagtga agctaaatgg
     9061 agtgcggcac gtgagaaagc ctttaagaaa ttagcaaaag acgtcactgt ccaaggcttc
     9121 cgtaaaggta aagcaccaga acacttagtg cgcgctaaaa ttgatcccgt taaagagtta
     9181 gatgaagcag tgcacagttt actcgatgaa gctttccaat ttgcgattaa agaaagcgga
     9241 cattatccag taattcaacc tacttatgac gtcgttaaag taagtgatac tgaacttgtc
     9301 cttaaattta ttattgttgc tgcaccagtt gttacacttg gccaatatga agggcttaaa
     9361 atcggtcgtg gtgaggctaa tgttgaagac agtgccgttg aagtacgcct taactcaatc
     9421 ttagagcaaa atgccgaact tatcttaaaa gaagacgctg ctgctttagg tgacactgtt
     9481 gtgcttgact ttgaagggtt tgtcgatggc gaagcttttg aaggtggtaa agccactaat
     9541 cacgaattag aacttggcag tggtcaattt atcccaggtt ttgaagatgc acttgtcggt
     9601 gtaaaagcag gtgaaagccg tgatgttaac gttactttcc ccgaaaatta tcatgaacaa
     9661 ctcgctggta agaaagctgt ctttaaaaca acagtccatg aagttaaaga aaaacgttta
     9721 ccagcgttaa ctgatgactt tgcagcctca cttaatatgc caaatgttac ggatgttgct
     9781 agtttacgcg tccatgtctt atttgaactt actaaacaag ccgaagccgc tgaacgcaat
     9841 gaatatgttg aaaaaatctt agcgaaatta cgcgaaacat caacctttga acttgcgaat
     9901 gacattattg accgtgaagt cgaacatatg gatgaaagac ttaaaaatca agtcacgcaa
     9961 caaggaatgg aatggaaaca atatttagaa ttaacaaaac aaactgaaga agaaattaaa
    10021 gaacgctttg tgagtgaagc aaccgttaac ttacaaaact tcttagtgct taatgaaatt
    10081 gccaaagcta aagaaattga agttactgat gcggaagtcg attttgaaat tgcgatgatg
    10141 gcgcaacaat ataacatgga agaaaaacgc atccgtgaat tacttggcga taacatcaac
    10201 aatttacgta atgatgtccg ccaaaagaaa attattaatt tcttattaga aaataatgaa
    10261 taattattaa ttagttaagt tttacataaa gaaaaggaga gttacatggc taacaaaaat
    10321 acagcaaaac gctatgagtt accgcttatt attacacgca atcaagtgta ttttccgttt
    10381 aatcaaagtg atgacttaga tgcgggacga ccggcgactg ttgaagcgat taaatacgct
    10441 aataatagtg atgataagtt actaattgta gcaacgcaaa aggtttttac ggttaataac
    10501 ccatcgatga atgatattta tgaagtcggc gtactcgcac gactaacaaa agtgcgtgaa
    10561 agaccgcatc acattaccgt acaacttgaa ccacttgccc gtgttttcat taatgaactt
    10621 gatgaaagtg gtccctttat tattgctgac gctactgtta cttttcatga gccacttgac
    10681 cctgacgcta atcctgaacg cttaaaaaat gccttatttg atttctttaa tgacaaatta
    10741 gagttaacaa gacggttaca tatcgattta gatgccttaa aggctgccga tatgaattta
    10801 gaagaactta tttacttctt agcgaataaa acgctcaata gcaccgaaca aagaatggct
    10861 gtccttgccg aaaatagtct agagcgacgc attgatattt tactcaagtt tcttgaagac
    10921 gatgtcgcta atgatgctgt tggagcgatt acacaaattg tgcgtaaccg cccacaaagc
    10981 ggtgaaggac ttgacgaagt tgaacgcgac ggcttaccgc tagaagacga tgaagatgaa
    11041 gacgaagacg aagaatttga tactggtgaa gaaattctag cgcaacttaa aaagcgctac
    11101 ttccccaaaa acttcgtaac gcgcattaaa aaagaagcga aaaagatggg ccgcgttgaa
    11161 ccaagtgaac gctcacggat aattgaatat ttagattggt taatgaagtt accttatgac
    11221 caagaaacaa ttgataatct tgaccttgaa aatgtccgta aggtcttaga taaagatcac
    11281 tttggtttag aagaaccaaa aaaacgcatt gtcgaattta tggccgtaaa acgccttgct
    11341 aaagaaaata gagcaacggt tttatgtttt tatggaccac ctggcactgg taaaactagt
    11401 ctagcgacaa gtattgcaac cgcgatgggc cgtaaacttg tcaaagctag tttaggtggg
    11461 gttgatgacg aaagtaagtt acgtggcttt ttacgaactt acgttggtgc gcaacctggc
    11521 attattattt cctcaatgcg taaagcagga acgattaatc caattttcgt ccttgacgaa
    11581 gtcgataaat tagggaagag caatcgtggt gacccaagca gtgcactttt agaaattctt
    11641 gaccctgaac aaaataaagg ttttatcgat cactatattg atgaaccata tgacttaagt
    11701 aaagtcgtct ttattgccac cgctaactat tactgggata tcccgcgtcc acttcgtgac
    11761 cggatggaaa tgattgaatt aaaagggtat accgaagaag aaaaagtaca aattgctgaa
    11821 aagcacttaa tacctaacgg gattgaaagt cacgggctta agggctatga cattaagttt
    11881 actgatgaag ccttacgctt tattatcggt ggctatactt gggaagctgg tgtacggatg
    11941 cttaatcaaa aaatcagcgc cattttccgt aagctaagtg ttgaagtact tagtgacgtg
    12001 gaaagtaact ttacgattac ccctgaagaa gtgcaaaagc atttaggtaa accgcgtaac
    12061 ttctttacca aaaaagaacc agaaccaatc gtcggtgttg tcactggcat cagtgtcgct
    12121 ggtaatattg gtgacatcat gaagattgaa gtaacaacat ttaaaggtaa aggtgttgtt
    12181 aatgtaacag ggaaccttga aaaaatgctt aaagaatcag gggaaattgc tgcaagccat
    12241 gttagaagtt atgctgaaaa gtatgggatt aatcctgatg tcttccttga cactgatata
    12301 aatgtacact tcccacaagc aagtacgaaa gatggcaata gtgcgggtgt cgcaatgacg
    12361 attggcatta ttagtgtatt aactgatcgt aagattaatc caaatgtggc gatgactggt
    12421 gaagtaactt taactggccg agtacttgcc atcggcgggc tttatgaaaa gattttaggc
    12481 tgtattagag cggggattga aacagtatta ttccctgaag aaaatgttgt tgaccttgat
    12541 gaaatcccgc aagaagtgaa agataaagtt aaacttgtgc cgattaaaaa tattgatgaa
    12601 gcagtagcca ttgcgctttt accgaaaaaa gtaaagaaaa aagcgactgc ctaaaagtac
    12661 tatgattaat tttcaaaata cattgttcgt tggtagtttt acaaacaaaa acgaaggggc
    12721 aatcgaagct ttgcccgaga ttattttcat tgggcgtagt aatgttggta aaagtagttt
    12781 aattaacgcg attattaatc gtaaaaactt agcgtatact tccaaaaaac ctggatttac
    12841 gaaattatta aactattata atgttgatga tacattttat ttagtcgacg ctcctggcta
    12901 cgggtttgca aagcgtgaaa aagatttaag cgctagtttt gcaactatga tgaataatta
    12961 ctttaataaa aacactaatt tatccatgat ttatttacta attgatagta gacgaacact
    13021 aaccgcggat gacgaagctt ttttagactt tgttagtcat cataaatttc cgctgaccct
    13081 tatttttaca aagtctgata aattaaatca aagtatgcgc gcgaaagcct taaactttta
    13141 taaaaagaca tttaaaggtg aagttaaagc gatttttact agtagtcaca ataaagaaaa
    13201 tataagtaaa gtgcatgagc atattagtga tacactcaaa aaccttaaag cataagtaac
    13261 ttgttgcaat taagtgctta agagtgataa aataaacagg cattgtcatt acagaagtaa
    13321 gattacgagg cgacacagag agtcgagcaa ggtgaaagtc gacacgcagc cacttactgg
    13381 gtcatagtga ctaaagatgt ccgggtaatg tccgttatca catctaatga agtgcgtata
    13441 catatatacg aaataaggtg gtaccacgga gcaattcgtc cttaaagtgg tactttttca
    13501 tttattggag ggaaatatga aacttgatac ccattataac catctaaagg tggaaaaagg
    13561 tcgttatgaa cactggaaaa aaagcggcta ttttaaaagt ggtgatacat ctaaaccaaa
    13621 attcagtgtc gtgatcccac ccccgaatgt tactggtgtt ttacacttag gacatgcttg
    13681 ggacaataca ttccaagata ttattgctcg ctataaaaaa gcgcaagggt ttgatgtttt
    13741 atatttacca ggaatggatc acgctggtat tgctacacaa gcgcggattg atggacggct
    13801 tcgggaacgc ggcttatcac gttatgactt aggacgcgaa aaattcttag aagaagcatg
    13861 gaaatggaaa gaagaatacg cgagtacgat ccgtagtcaa tgggcaaagc tcgggctcat
    13921 gcttgattat gaccatgaac gttttacgct tgataaggga ctaaatgacg ctgttaatca
    13981 tacatttgtg gaactttata accgcggtta tctctatctt ggtgaacgga ttattaattg
    14041 ggaccccgtt ttccaaacag cgcttagtaa tattgaagtc attcataaag atgttgaagg
    14101 ctcattttat tactttaaat ataaacttga aggcagtgac ttttatttaa gtgtcgcgac
    14161 aacgcgaccg gaaacaatgt ttgccgacgt cgcccttgtg attaacccac gcgacgagcg
    14221 atacacaaaa tacttaggta aaaacttcgt aaatccagta aataatgaat taattccgtt
    14281 aatcgctgat gattatgttg atttagaatt tggtactggt gtgatgaaat gtacgcccgc
    14341 gcacgacgta aatgacttta aaatcggtga aagacataac ttaagtcgcc cgatttgttt
    14401 aaataaagac gcaacactta acgaacgcgg tcttgaacta gcgggtctag atcgcttcga
    14461 tgcacgcgaa aaactcgtcg ccaagatgaa agaacttgat ttacttatta aagtggagaa
    14521 aatcgtgcat aatgtcggtc atagtgaacg aagtgatgcc attattgaac cgtatttatc
    14581 aaaacagtgg tttttacgca tggatcactt tgctaaaaaa agtatcgaag ggcaaaaagg
    14641 tgatgacccc atccgctttt tcccgaaacg ctttaataaa acttatttaa attggatgga
    14701 gattgctgaa gattggtgcg tatcacgcca aatttggtgg ggtcaccgta tcccggctta
    14761 ttatcataaa gaaactggtg aaatagtagt aagtgttaca ccacctaaag acttagaaaa
    14821 ttatcggcaa gatgaagatg tccttgatac atggtttagc agtgcacttt ggcctttttc
    14881 aacgctagac tggccaaata cggatagcga actctttaaa cgctattacc cgaccgatgt
    14941 aatggtgaca ggctacgata ttattttctt ctgggtatca cggatgattt tcatggggtt
    15001 agagttaact aatgaaaaac catttaaaga cgtcgttatt cacggtttaa ttcgtgatga
    15061 acagggccga aaaatgtcaa aatcactcgg caacggggtt gatccaattg aagtgattgc
    15121 gaagtatggt gttgatagcc tccgttattt cttagcaggc gcaagtaccc caggccaaga
    15181 tgcgcgctat agtgaagcgc gtatccaagc aagtgtcaat tacttaaata agatttggaa
    15241 cgcagctagg ttcttacttt taaacttacc agaagacttt aaaccaggta agattaatca
    15301 taagaaactg catcctgttg atgcttttat cttaagtcgt tattacgaaa cagtgcgtaa
    15361 agtcacaagt ttaatggata agtatgagct tggtatggct agtaattatc tctatgcttt
    15421 tgtttatgat gattactgct caaattatat tgaaatggtt aaagttattt taaatagtga
    15481 tggtgatcat acaaatacat ataatgttat gtatcacact ttacacggaa tcttaatgat
    15541 gatttatcct tattcacctt ttattagtga agaaatttat ttagcgctcc caaatcatct
    15601 cgattctata atgttagcga gttatccaaa agctagtcgt ttacgcttta agcgcgaagc
    15661 ggaagaagta aatacgatta ttaacttaat taaagaaatt cgtaacttta agagcactaa
    15721 taacttagcg cccaatacta aaatgaattt atatgtcgct gatgttagct ttgatttaac
    15781 gccttatctt gatattattc gccgtttagg ttttattaat gaatttaagt atagtgatgt
    15841 tcctattgaa gtaacgacag ttagtcttcc aaaagctact tttggcttag agttagatgt
    15901 tgatcgcagt gtgctgatta gtcaacttga aaaagaaatc gaaactttaa cgaaagaagt
    15961 tgaaaggagc actaagcttt taaataaccc tggttttaaa aataaagctc ccgcaagcaa
    16021 agttgcggaa gaagaagaaa aagcccgcca ttacgctgaa agtctggcgc tcgcggttga
    16081 taaactaact aaattgcgat aaaacattaa ctttcctttt acttaatagt aggaggaaat
    16141 tattatgact ttattaacta atgccgaaat ggcaaatatt aaaggtggtg aaccaatcac
    16201 tttagcagcg gtaatgacaa ttttagtcat cgctattgta acggtgattg tttataagtt
    16261 atttacttca aacgccggtt ctacgacaat accgggaggt tttaaatttg aatggaagta
    16321 aacgcattaa ggagttaccc catttaaaaa gaccgcgtga aaaggcgctt tattacggga
    16381 ttggtagttt aagtgacgcc gaagtacttg cgctgttaat tgggaaaggc acaacgaaag
    16441 ataatgcctt aagtcttgct aagaagcttt taaataaaca tatatcctta caaaatatgt
    16501 atcggattga tgacccgaag ctccttttaa tgccaggaat tggtgaagtt aaggcactgc
    16561 aaattttagc ggcatttgaa ttaaataaaa ggcttttgtt tcttgaagga agtcacgacc
    16621 gcccaatata tcgcgaagac gaaattgtgc gttcctttca aaggcggatt ggacgcttac
    16681 aaaaagaaac gctctacctt ttagcgctca cgaatcgtaa ttatattatt agtgaaaaac
    16741 aactatattt agggacggaa aaaggcttta gccttgaccc taaagaagta gtaagagaat
    16801 tattaatcgc taacgctgat cgttatattt tgattcataa tcatcctagt ggtcatgttg
    16861 aacctagcgc aaatgactta gaaacaacga tcgagttggc aacaataagt gcccaatttg
    16921 gtgttactct ttatgaccat ttaattattg gtgcaaatgc ttatttttca ttaagaaagc
    16981 ttggtaagtt gtaaaatggt attattttag tagttgttta gtagataata tgttttaaca
    17041 ttattattta tatctctata ctattatgtc tgcgattgtt tttaatatct caattgtatc
    17101 aaagaattta atatcggctg cataactatt tttttgcatg aacttttccc actcattaat
    17161 tagcttacta ttgttttgca tattatttaa tatttctttg ctatccttaa taatctttgt
    17221 tgtctttctc ttgttggaag tattaaaaat agctcttttt aaagttttaa aattaattgc
    17281 attatgttta agtttaaaaa tcatataaac atcgtaaaaa tctttcattc ttgttgtttc
    17341 tatattgcga acaattattg tttcatattt ttctgcgatg attgtttcaa tattatatgc
    17401 atataacctg attttttctg atgttaagag acacgaatat tcatatacta ttttgcttgg
    17461 tgtaattgga tcaccagttg ttatatctag tttaagagga attctagtat tttctaacat
    17521 tgctacaagc gatatacgaa agttatcata gtaatcgatt tccctaataa tttcgatgtt
    17581 tgtaaaaata aattctacgc catcatttat gtctatattg ataatttctt tgatgacatt
    17641 agttatcatt tcttgttcta aattcatgtc tttaatattt gtatctatat caatagttgt
    17701 cctattagtg attccgataa ttgaagatat taaaaaccca cctttaataa tgaatttgtc
    17761 tttatagagt gattttgaaa gccgcatcaa aaacctctca aagaagtaca tccttaaaat
    17821 tatatttgca cttaaaccga tttgtgaagc aagatttcgt gttttagctt taatacttgt
    17881 aatattaatc atgaaagaat ctcaatatag cgtaaaattt cattttcaat cttaaattga
    17941 cgactatatt tgattaactt tttgatatct ttatcttttc gacgaaaata tcttgtaatt
    18001 gcatcaacaa aaacttcttt ttcgatattt tctcgatcag cgatgatatc acaaatggtt
    18061 ctttccatat cataaactaa aattttgttg ccaaaatatg ttttcatcgt aataataccg
    18121 attttatgat gttttctttt gacatatctt gtttttaaat ttatatatct tttcttaata
    18181 tgtgaagtgt tatatccttg tggtactgtg atgtagagtc tatctggcat tcgatctgat
    18241 aaatcatgga gaataagagc ggtctcatgc gaaaaacaaa cctgattact attagcagaa
    18301 actaacatga aatcatcaat aacttcgccg gccttttgat agacaccagg tcttagtctc
    18361 tcaagttttc cctctttcac taatactgaa agtagttggc gattaacacc aattctagca
    18421 gcgtctttat tagtgattac tggcctttca tttaagtagt tttgtaaact tgtcatagca
    18481 tcctttctta cactttcgct ttaattatac gaaattaaag agtaaatgta aatatatgta
    18541 agaagcactt ccacattact taatagtcaa gaggtagtgc tttctcaata taaaatacaa
    18601 tcgaaataag tatttttact acaaataaat gatttatatg actgtttaat gtgaaaaaat
    18661 cattgtttta tattttaacc cttgcctaaa cctatggttt atgatatttt acatatgtta
    18721 tcgcctcaca agctttaacc gcctagaaat ggttagtgtg caaagaagaa caattaactt
    18781 ctaccataat agcgagtcca agtggaaata tataaaggag gttagcacat gtacgcaatt
    18841 attgaaacag gtggcaaaca agttaaagta gccactggtg atgtaattta tgtcgaaaaa
    18901 ctcgatgttg ctgaaggaga agatttcgtc tttgataaag tcttactcgt cggtggtaaa
    18961 aaagctaaag ttggaacccc atatgttaaa ggtgccaccg ttagcgcaaa agtagtgaaa
    19021 cacggcttaa atcgcaaaat taaagtattc acctacaaga acaaaagtaa tgttagacgc
    19081 atgcttggac atcgtcaacc atatacttgc cttgaagttg tggaaattaa aggttaagta
    19141 tgattaatgt cgttattact acaattgatg aagaaatctt aaagattcgc attaaaggtc
    19201 acgcttatag cggagaacca ggtcatgatc tcgtgtgtgc tggtgtaagt gccgtaagta
    19261 ttggtgcact taatagctta catgaagttg atactgcctt caaagttact gttgaagatg
    19321 gtctagtgga aatagtccca ttatatagac caagttacga aaacaagatt gtgctgcaag
    19381 tattaataac gagcttaaaa actattgcag aatcatacgg aaagtatgta caaatccgtg
    19441 aagaaaggag agcagaagca tgaaattcat tctaaactta caattatttg cctcgaaaaa
    19501 aggtgttgga tcaacgcgca acggccgtga ctccactggt cgtcgcttag gtgctaaacg
    19561 cgctgatggt caactagcga aagctggggc aattttatat cgccaaagag gtactcggat
    19621 ttatccagga cttaacgcca gaagaggtag tgatgataca atctacgcga ctgttgaagg
    19681 tattgtccgt tttgaacgct ttggtaaaac acgcaaacgt gttagcgttt atcctgttaa
    19741 ctaattagag aaacaaaatg accaccgctc acttaagtcg gtggtctttt tttcatatat
    19801 aatataaaat atattattga aaagaggtac acatggctaa aaaattagtg acggttgtcg
    19861 tcccaatgta taacgaagaa ccgatggttc cccatttttt aacaaaaatc gccgaaatta
    19921 ttaaagaaaa tgatggctac aactttgaag tagtagcggt taacgacggg agtcgtgata
    19981 atacgctggc cttactttat gattttcaga aaaaatataa atttttacgt gtcgtaaatt
    20041 taagtcgtaa ctttggccac gaagcagcag tggcggcagg actaagtgtt gccaagggtg
    20101 acgctattat tccaattgac gctgatttac aagatccacc ggagcttatt aaaccgatgc
    20161 ttgaactttt tgaagaagga tatgatgtcg ttaatgcccg tcgtgcttcg cgtaaagaag
    20221 atacatggat taagcgttac actgctggta aattttacaa agtaattgat aagttatcag
    20281 ggaaagtacg gattccgcaa aatgttggtc attatcggct tatatcaaga cgcgtgcttg
    20341 atgaagtttt aaaattaccg gaaagcaatc gcgtctttag agtagaagtt ccttatgttg
    20401 gctttaaaac agcggaagta gcgtttgtta gacccaaaag agaacatggt aaaacgcatt
    20461 acaatatgaa gtcaatgatt gatttagcaa ttaacgctat tacaattacg agtactaaac
    20521 cacttaactt tgtctttaag tttgcaatgt ggctggggat tatctttggt ttaagtgccg
    20581 tgacgcaact tgtgctattt attttagaaa tgactaaggt attagtgctg ccttttctta
    20641 atcacggtgt gtggttattg ataaatgtta gtttattttt aacaacagtt atccttttag
    20701 cgcttacaat aatctcaatt tatcttggca aaacatttaa tgaaacgcaa aaacgcccat
    20761 tttatgttat tgaatcagta aaggaaaata aataaagaat gtttatcgac caagctaaaa
    20821 taacacttaa agcgggtaat ggcggtaatg ggatggtctc ttttcgccgt gaaaagtatg
    20881 tgccaaaagg ggggccagat ggcgggaatg ggggtcgcgg cggttccatt tactttgtcg
    20941 ctacaagtaa tatttcaaca cttactgatt ttcgctttgg tttaacgatt aaggctaata
    21001 acggcggtaa tggcgcaact aaaaatcagt acggtaaaag tggtgaagat ctctatgtca
    21061 aggtaccaat cggcactgtt gtttatcttg aaccaagccg cgaagtaatt gccgacttta
    21121 aacttgaggg cgaaacaatg ttaatcgcta aaggtgggaa aggtggtcgc gggaatgctg
    21181 cttttaaaag tagcacgaat cgcgtgccgc gtattgctga gcacggtgta cctggtgaag
    21241 aactacttgt gacacttgaa ttaaaattac ttgcggatat tggctttatt ggcttaccta
    21301 acgctggtaa atcaacactt ttaaccgtga ttagtgaagc acgaccacaa attgataatt
    21361 atccttttac aacacttaga ccagaactag gcgttgtaaa agctaatagc ggtaaccgtt
    21421 ttgtggctgc ggacttacca ggcattatta aagatgcaca tttaggacgc ggacttggtt
    21481 tacgtttttt acggcatgcg cagcgttgta aggtgctcgc tttcatcatt gacggtgaaa
    21541 atagtgaaga ttactataaa gaatataagt tactgcgtaa tgaagtgaag gaatatgggt
    21601 ataaattaga tgaaagaccc ttctttgtgg ctgtaagtaa agatgaaaca gtagatactc
    21661 ataaaaaagt aaaagcacta gcgaaaaaac taaaagatat taaagtgctt ggttttagta
    21721 gtctcttaca tgaaaatatc gatactttaa ttaatacgat ggaagaatat attgttaacg
    21781 ctcccgaaat tgaaatggtt gatgaagaag gcgttgcggt taagtatcga gagtacgact
    21841 taacggcgac tattgaccct gatttattta cgattgagaa agttagagcc ggagtgtacc
    21901 gtattgttgg ggaaaaagtt gaggaaagat atcataaata catcttaaat acagaagaag
    21961 cgattttatc acttttaaat tatctccgtg aaattggtgt cgaagatgcc cttgataaaa
    22021 tgggagttaa tgatggtgat actgtgattc tcgttgactt tgaatttgag tatttccgct
    22081 agggaggaat tatgggacaa gaaagtttac aaaattattt acagtactta gtgcgttact
    22141 taaagcgtag agcacgcgga tttaatggtt atattttagg gttaagtggt ggtctcgata
    22201 gtgcagttgt ggcacttctt gcccaaaaag ccgtaaagga cgacttatta tgcgtcatta
    22261 tcgatattga aagcgaaaag tgtgatatgg atgatgcctt agaactttgt aaactacata
    22321 atttaaatta ctattacatt gatttaactg cagaataccg cgcggttgtg aaaaaacttg
    22381 aagaaagtaa aaagttaacc gaacttagta aaattaatac gaaagttcgt ttacgtatgg
    22441 tgacgcttta tgctttaggg caaacacata gcaaattagt gctcggcact gataataagg
    22501 cggaactctt tactggttac tttacaaagc acggggatgg ggctgctgat ttattcgtcc
    22561 tttcaagctt aacaaagggt gaagtctttg ccgcagcgcg cttacttggt gttacgccaa
    22621 acattattaa taaaattcca agtgctggac tttatgtcgg acagactgac gaagccgaga
    22681 tgggcattaa atacgaagat cttgacgcct ttatcagcgg taaagaagta tcgccaagcg
    22741 ttaaagcacg cgcaatagaa ctgcaccgca tcagcaatca caaacgacgt ccaatccctc
    22801 gaccacggga gaaaagatag tgaaaaagca aaaatttgtg agtaacacga ttattagtgg
    22861 cctagctgcc gtacttttat tcgtcgtgtt acttatattt ataaggatgg tgggagtatg
    22921 atttatggat taaaaggcat tgttacaaaa caaacggcga caacgattgt cttagatgtg
    22981 cgtggtgtct attacgaagt taaagtgccg tccgtgccac agcaacaaca gtttagtgaa
    23041 ctatttttat atacatatca agtaattaga gaagatgagc attatcttgt tggcttttta
    23101 agtcttgaag aaaaaagctt atttaccgag cttttaaatg tttcaggcat tggtccaaag
    23161 actgctttag ggatcatgac ggccacaaca ccagagcgct tcttacaggc agtcacgagt
    23221 gaagaactta gttacttaaa gaaattaccg ggcgttggtc caaaaggcgc gagtcaaatt
    23281 attctcgact taaagggtaa attacaaact gacacttcaa aaggcttaaa tccttatgaa
    23341 caagaagtaa cgcttggatt acaaagctta gggtttaaga aaagtgaaat tactaaagtt
    23401 ttaaagaaag ttagtaataa gtcgcttgac gctaatgagt tattacgtct agcgcttagt
    23461 gagttaagac ggtaatgagt aacagattag tgagtgataa attagatcaa agtgaagcaa
    23521 gtgtggaatt aagtctccgc ccgttaacat tgaatgaata tgtcgggcaa actgcgctta
    23581 aacataattt aaatattttt atcactgccg ctaaagcacg gaatgaagta cttgatcata
    23641 ctttactttt tggaccgcca ggacttggaa aaacaacatt agcgcacatt atcgctaatg
    23701 aaatgggcgc aaatattaaa gtaatcaatg gtccaagtat tgaaaaagcc ggtgacttag
    23761 cagcgatttt aacgacttta gaagcaggtg atgtgttatt tatcgatgaa attcaccgtt
    23821 taccgcgtgt cgttgaagaa gtcttatata gtgcaatgga agactttaag ttacaagtga
    23881 tgattacgct tgatggtaat gttaaaaatt tacaagttga tttaccaccc tttacgcttg
    23941 ttggtgcaac aacaagagcc ggtgacttaa gtagtccgct ccgggcacgc tttggcatta
    24001 gtgaaaaaat tgattattac gaagaaagtg atatttttaa cattattaaa cggacaagtc
    24061 gtgtgtttga attaccgatt aatgatgacg ctgcgcttga aattgcacgc agaagcagac
    24121 gaacaccgcg cattgctaat cggctctttc gccgcattcg tgactttgcg actttcgcta
    24181 gtaagcgagt gattgaatac gaactatgtc ttgaagcttt aaaacattta cgtgttgata
    24241 cgcttgggct agatgaagtt gatattgctt atttaacaac actagtagaa cgctttcgtg
    24301 gtggaccagt tgggcttgaa accctagcga acgcgattgg tgaagaagtg acgaacttag
    24361 aagatgttta tgaaccatat ctcttacaaa ttgggatgat tgataaaaca ccgcgtggtc
    24421 gtgtggccac agaaatttgt tatgcacacc ttaaaaaacc acgacaaaca catttatttt
    24481 aaaaaaatgc cgatattttc actaattagc gttattttgg ttgtgaaaaa agtcatttaa
    24541 tagtaatcta ttaaagcgat tatgtgtatc gaagtatttt tagtatgacg gaggaattct
    24601 tatgcgtaga ctaacagcat atctaacatt aacatttaca ctgatttttg gagccgcgct
    24661 aaatatgttt agcccgctcc gtaatttagt gagtaaccac gaataccaaa ctggtcgtga
    24721 gtttgtttat cggattagtg ataaagatcc cgataaagat ttagatccaa aagcaattga
    24781 tgagattgtg acgattatgg accgccggat gcaaaattat ggcgttagtg aatacaatat
    24841 tgctaaagaa ggcaaaaaca ttgtccgtgt ctccacaacc ttacaatcaa ccgaagaata
    24901 taaccgtctt caagtttatc ttaactataa cgctaatttc acgatgaaaa ttggtgatga
    24961 tgataatacg gaagcgattt taagcgctga tcaaatcttt gccgataaaa aagcccgtgt
    25021 tgaatatcgt ggtccttatc cttttattgt cttcccgtta agtgacccca ctttatttga
    25081 aacgacaatt gttgatgtcg cgcaaaaaat tcaagaagaa aaaggttcac aagaaacagc
    25141 agaaggcgaa gataagccag ctttagtcaa agaagcaaca atcgtcttat ggagtgatta
    25201 tgaccctgat aaagatagtt atatgagccc agttggcgat acacaaggta aaattttcct
    25261 ctcctttgac tatcgctcga tgtggtggaa tgaagacaag accgaaatcg gtgtcgctag
    25321 tccgcttgaa gggatgtcgg aaaaccataa atatacaacg gctcaaatta aaaaagctag
    25381 tgaaaccgcc cgttatatga tgaatgtctt taacgctggt acacttgatt ataaagttga
    25441 tttcttatat gccaataaag caatggcgcc aacggttgaa ccacttatta atttaggtac
    25501 acgcgataat attgccttaa gtgcaacatt ctttgctagt ttagtaacaa tggttgtcgt
    25561 ccttgttatt ttatttgcga tttaccgttt accagtctta aatgcactaa gtagtagttt
    25621 attaacattc ttaggcacaa tgattatttt caacgcgatt caagtcgaat ttagtacaag
    25681 tgccattatt ggtgtactga ttgtgacctt tgtaagcatt ttaagtacga ttttatacgc
    25741 tgctaagttt agaaatgaag tgtataaagg ccgcaccttt aaaaaggccc acactgaagc
    25801 tattacccgt tcaagtttct taacaattga tattttagtg ttattactcg tcacgggtat
    25861 ctttacatat ttctttggtg gtgcagcact cactgggttt gctacaacct taatctttgg
    25921 tagtatcgtg atggtggcaa gtgttttaat ccataacagt ttactcttac cacttttagc
    25981 ccataaccgt gtaactagta aaagttatac ttattacggc attaatgaaa gcaaagtacc
    26041 tgatacttta aaagatgaaa aacaaacata ctttgggcgt tttgttaatc ataaacccga
    26101 aaatcgcgcg aagcttagca tgattttaac tggtagttta agccttatta ctgctgtttt
    26161 acttatcgtc ttcagcgctg ttaatattaa cccatacaac ttagtgaaac acgacgaaaa
    26221 tattacgcgt gtttacttcc aagtcagtga aaatagtgat attaacccac tttcaccaac
    26281 cgcaagtcct gttactttac ttaattatat tgagttagac ggagaagcct taacctttga
    26341 aaagaaagaa ggtgcgccag tttacgaaac gcaagtttat aaaacaatgg aacttgatat
    26401 tgaagacgaa attgaagtta cttacaattt ctatgtttat aacttaattg gtgtttacac
    26461 tggtgaagaa gaagttaact ataaagtgac tgctgacgcg ccaatgcaaa gtgcaacgtt
    26521 aagtgaagcg cttgatatta ttgttaaaaa tattgacgat aaagccgtta tttcctttaa
    26581 cgctgtcgaa aaagaagcaa ttacgccgca ctttggccgc attgcttggg ttagtttagt
    26641 aatccttgct gttacttctt tctacttaac aattcgcttt ggacttacgc gcggtattac
    26701 aaactttgtt gaaagctttg tcgctgctag tttggtgttc ctattcttaa tggtaacgcg
    26761 tctagcaatc ccgccactag cgacaatggg agccttcgtt gcgattttag ccgttagttt
    26821 cttaaatgtt gtgatcttta caaacatcaa agatgtccaa gaagaaaccg ctagtaaagg
    26881 gcttacgcac cgtgaacact tcgaacttgg acttcgttat agtttaagtg tcatttatat
    26941 tactgtctta atgtttagtc ttgggttctt agtctttacc ctgattggtc cgcttgctgt
    27001 tagcagtgtc taccttgcta gtgctgtagc gatggttgcg gcactcctcg tcgttgttaa
    27061 atatagtacg gccttcttaa gcggttcgtt aaatgtcctt gatcgcttac caaaacttga
    27121 acgtaaacaa aaaacagtca ctggtaaaca acggacaagt attagaagta atgaaccaga
    27181 agaagccatc tttattggca ttaatgacta atattaatta aataaataca aaaagtacta
    27241 cccgtgtggt agtatttttt tatgaataaa ttcctcgata aattattaaa gtattacgac
    27301 ttatcactaa gagattacga aaaattaacg gcagaagtca ctaaagaaaa tttaccaacg
    27361 gccgaagctt ttttcaactt tgaatctttt attagtcgga ttgaaaaagc gatcgctagt
    27421 gatgaaaatg tcgttattta tggtgattat gatgctgacg gcattcttgc tacgagtatt
    27481 ttaaaatatg cctttcttaa acgtggtaaa gacgttttta cgatgattcc cagccgttac
    27541 aaagacgggt acgggttaaa cactaatgtg gtcgaaaagt ttagtaaacg cgctattaat
    27601 ttaattatta ccgttgataa cggcattaat cagcatgacg caattgatct cgctaatgag
    27661 ctcggtattg atgtacttgt gagtgatcac catgagttaa gtgaaacgct cccgaatgct
    27721 attgcgattt tgcatcctga actaagtaat ttggtacgtc ttaatagttg tggcgcgtat
    27781 atggcgctag ttataagtta cgggatgcta ggatattatg atgattattt aattagttta
    27841 gcggcgattg caacactggc ggatatgatg ccactaatag accgtaatcg tactcttgtc
    27901 cgtctagcga taaaggccac taatgaccac caatatttcc cgcttgttaa attaaatgac
    27961 agtacctttt ttgatgaaac aagtatgagt atgaaaatag cgccaaaaat taatgcatta
    28021 gggcgcttaa gtcaaaatta tgaaagtaat gtgttagttg aatactttac gacgactgat
    28081 gttaaacgcc attatgatat aagtgactac attgaaaaag tatataagaa gcgtaaagcg
    28141 atgagtcgcg gagaagaatt aagcagtgaa gagttaaaaa atccagtaat tgctttaatt
    28201 actgatgaat tagaaggcgt acttggactt attgcccaaa attatatgct tgaacataat
    28261 aaaccagcct ttatctttac cgaaagttca gaagaccctt ctttacttaa aggtagcgta
    28321 agaagtaaaa gcggttttaa tgttgttgat gcttttagtc atttggaaca tttaatactc
    28381 acaagtggtg gccacaaaga agctgggggc ttaacgatta gaaaacaaga ttttatagtt
    28441 tttaaagaag cactgaataa atacgcgaaa aattatccgc ttataacgca agacgaaaaa
    28501 tatttagatg tcacgcttga cgaaattaca aaagaaaatt atgcactcat taaaaatctt
    28561 tcaccatttg gtcataagtt tagagcgccg ctattgcgcc tcacttccct tacaaatgta
    28621 aaatacttgt tttcgcgtga tgggaaacac attattaccc cacttgaaag cggtgttaaa
    28681 ctaattggct tctctttagc ggaagaaagt aaaaatgcac ttaacatcgg ctttactgct
    28741 acttttgccg aaagtgaatt ccgtggccga agaagtattg atttagttat caaagaattt
    28801 ttcactgcct aaatagtaat aattaacaag taattatttt ttgtcctttt tttttgatta
    28861 tgttatgatt aatatgtagt aattaaagga ggaaaataat gaaaagaaga aaattacttt
    28921 tactaccagt tttcttgctg ctactagccg gatgtaaaga tacatcatct agtgtcagtg
    28981 ttgaagactc ccaacccact gtcacaagta caagtgaggt tgaaccaagt agtgaagagc
    29041 cacaagttac ttcagttgaa gataccgaag ctccaccaag tattcctact agtgaagcac
    29101 cagtatttga acacaaaaca attgaagaaa ttcgggcaat gaccgaagac ggtgcttccg
    29161 caacacctga tcaaaagcaa ttgttctacg aaacttcagg tgtcgtcacc gcaaaattcc
    29221 gcggtgctaa catcagtggt gtcggaccag cttggaacat tgcaattcaa gatggtgaac
    29281 acgcattatt actatatgct gtaaccgaag aagcttacgc tgcggacttt gctgatgtac
    29341 caattggttc aaaattaact gttactggta acttagccgc ctataaaggc cttcgtgaac
    29401 ttagcaccat taaatatgtt agtcatgaag ctgcatctcc agtagaacca gaagtcctcg
    29461 caagcatcga acaagctgac ttagccggaa aagactccaa attagttaaa attgaaggac
    29521 ttaaactcca cgctaaacca caattagaag cttatgttga tggcgaccgt aaccgtgtta
    29581 atattaatat ggacttacgg aaagatggtg tcaaaattgg aacatatatg cactataata
    29641 ttccacttgc tgatgcccaa gccactgtcg aaaaattaca aaccgtgaca agtaatagta
    29701 ctgttacttg gactggtatt cttggtatgc acgaaaaggt tttccaactc acaaacgcaa
    29761 atgccgatga atgggaaatc gaagttggcg caccagttgt gcttgaaacc attgctttca
    29821 gagatgaaac aaaagaactt gcaccaggcg caacatatca aacaacatta gtgtcaacac
    29881 catttgacgc agtcgttggt gaagttactt acgctagtga tgccgcagca gttgctactg
    29941 ttgacgctga aggtgtcgta actgccgttg ctgttggtac tgcaaacatt accgctaccg
    30001 ctagtggtaa agtagcgaca atggttatta ctgttgctga cgctgctgaa ggagtttacg
    30061 atattactgt cgttgattca tataccacac cttccgaaat taaaaccgaa gaagcattcc
    30121 tcgccgcggt taaaggttat ccagttggca tgactgtacc agaagttgtt aaagtttatg
    30181 gtattgctcc aacaatgggt tctaatcaac ataatgatta caccgctacg actggattta
    30241 aattcagtag tagtaaagat aatgctgctt taaccttaaa gatgcctgct ggtgtcgaaa
    30301 ttgctaaagt aaaagttgaa ggtcgtgcct ggggtaaaga ca
//
LOCUS       DAOSLP010000008        29264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_1796_length_29264_cov_8.132596, whole genome shotgun sequence.
ACCESSION   DAOSLP010000008 DAOSLP010000000
VERSION     DAOSLP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 29264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29264
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1796_length_29264_cov_8.132596"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..411
                     /locus_tag="PK460_01510"
     CDS             244..411
                     /locus_tag="PK460_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53811.1"
                     /translation="MNKNRKWLARSAVISLLISAIITPAFFRKDAVKTKAATTIPVLV
                     GDSIGWEPSVS"
     gene            514..909
                     /locus_tag="PK460_01515"
     CDS             514..909
                     /locus_tag="PK460_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53812.1"
                     /translation="MEDLQFDTTPSLSRDYIYRYEDSPDANFITIYPGEIKVQVFNPD
                     NAGGSPAGARRMKVEYTSLLGATPSTPTGTSSYALRGPAVGDWTTHDINNCLVGISGY
                     DGRYLSLTRAFNADDFKIFVYKQTLNEPY"
     gene            961..1611
                     /locus_tag="PK460_01520"
     CDS             961..1611
                     /locus_tag="PK460_01520"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOE53813.1"
                     /translation="MPGDPSSGYHESFHVNTAGNYMVVYDLKMQKIYFHNAGTTYHKV
                     TKYYGELLIEEAAAFTGLNYTPAPFYQPEKKFVGWYEDALLETAYVPKDLTSDITLYA
                     KFVDSPSVNLRFYDRNLDIKVDNGGFNEYVVFAYGKNTDGTEPLGGWPGRMMQKVVDM
                     GHELSYGYYALNISGQDRPEKVTFNNGCQPFEPEHVSTPELVLPADKNLYEHDVAA"
     gene            1618..2016
                     /locus_tag="PK460_01525"
     CDS             1618..2016
                     /locus_tag="PK460_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOE53814.1"
                     /translation="MTPVHDAATSYAINFLYETRNCDATGAVNNISSHKREELGNLFN
                     ELAPDVQADLKNATATLSGDLVSQAMARYVYILQKYGTVTFTNFIDRTLPPSGIPAVR
                     GDNKQNFYVLGIMAFIAVAGFFFLNRKRRY"
     gene            complement(2136..3110)
                     /locus_tag="PK460_01530"
     CDS             complement(2136..3110)
                     /locus_tag="PK460_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53815.1"
                     /translation="MKNAICRIGELMAIAMGSLELVSMRIKEEPFDTSNTITRYTRET
                     SSTIRDSFFTTIDFKAAISDNTIIKGATLTADDSDMISLLNNDLYGIGYISYAALESS
                     ELKALMIEGVRVSEKSIIDDSYKLSRNFNYITKAPSDLSEKEYALITGFIAFSKSREG
                     LSIIKAKDGIVTQNIEEAESFTTLLAKSENSALAKMLYNTNEAPIHIKLGGSKSVESI
                     SRALSSAFTSLVPAFKAVHNYTSNEMAYEATQGSNKDSAEPLHIGFLARDLDINETEP
                     ALAGSYGVISKDAIVAVVSNKNHVLSDITRDQLKQIYKSETITWSALE"
     gene            3816..4490
                     /locus_tag="PK460_01535"
     CDS             3816..4490
                     /locus_tag="PK460_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE53816.1"
                     /translation="MSPLEIVLVSVLGALILIVAIYLFVVHTNAVNRLEIFEEVNKEA
                     KSGKIAFLGDSLTDFFPIQDFFPGVKIYNRGIAGDTTKNVLKRLDNVVALAPRKLFLQ
                     IGTNDLAKGSSPRKIVANIKKIITTFKKEVPNIEVICISLYPVSRTKKFLSTFIVGLR
                     TNKKIKKTNELLINFCAEEGLKYLDFYTLLADKKGRIDRRYTVEGLHISGKGYAVIAK
                     ALAPYL"
     gene            complement(4492..5268)
                     /locus_tag="PK460_01540"
     CDS             complement(4492..5268)
                     /locus_tag="PK460_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE53817.1"
                     /translation="MKKIVIPLVMLLSLVGCKTEKLIRVATRGCIHEEILNAAKTSLK
                     EMGYRLNLETIDNALLANSSLSSGGIDANIIQRTDDLIAYNNRVSKRAELTSAGRVYL
                     SALGVYSGTKTTLEDYNPGDKIVISDRAEDYERIMSFIEEVFTVTVFTEQEKTPNYRQ
                     TPLDFLTIEYMNPDLIPEAQKHPEEAFYIMTGSQAYNSGLSYEHILATETSNDYAFIV
                     GIRRGEENNDRIRALMKVLYSDEIGDFIVEHFRGLIIPYR"
     gene            5525..6235
                     /locus_tag="PK460_01545"
     CDS             5525..6235
                     /locus_tag="PK460_01545"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOE53818.1"
                     /translation="MVKQQNNLFLLNNLFNVRDLGEIDTKYGTKTRRYKYIRGTAKGT
                     MTEIEKEHFYNLGVRVVVDLRYTNEIEKTPSPLRGYKDIKYYHVDMMGEFWQMREQGY
                     KDMTDLYIDLLDDSQEKLLTIFKIFLKYPRDGIYFHCTAGKDRTGVVTMLLLDLVGVP
                     HSVIVENYSESYTNNLSRPGYKFIKPEWRRFILSEPEYMERTLDHLYTKYGGSRAYLS
                     HIGLRDDEINNLAATIVE"
     gene            6305..7060
                     /locus_tag="PK460_01550"
     CDS             6305..7060
                     /locus_tag="PK460_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53819.1"
                     /translation="MKTLANLLDNDTVTIVLFFAFLLLAVVIYLIVSKKEPSKEKMRP
                     EERALKMVEAPKTEVKTEVTPEKPVTEEVKTKKVEREAQKVKVKKEDTDDDQFAAIKY
                     SYSYTAQVSLAPEASRERYLKIKQEILSYTSVNNTISWREERFTLSGKTIVKFKLIGN
                     VLRMYIALTLEELSDVNIKIDDVSRYKEHELTPSLLRISGNTGVKRALQAIDILMQKL
                     ELKKDPQYKLGASDIPAAQTIDELLEAGLIRKI"
     gene            7064..7918
                     /locus_tag="PK460_01555"
     CDS             7064..7918
                     /locus_tag="PK460_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE53820.1"
                     /translation="MKKIALVLGGGGAKGSYQIGVLKALHETKILKRVRVASGTSIGA
                     IHTLLLMHDLSIEEMINVWKEFTNEAIYGRNKFKETWNISGFYNTKGILEKILPFTNE
                     EAFRNTKIEGYVTLAKVPTKGLFAKLNMKNYEKEVVFLNESEHPLKAVLASAAIPLVF
                     GEKEIEGTRYVDGGLVDNFPFTPALIANANIIISIGLDPKLLPTKPLPGRLHIDFTPL
                     EELGPFPKVSLDFSPEKTQQYFDLGYENAKALIAYLKREKFFWFGGRLKLRKGRLVTL
                     ETLTAKTK"
     gene            7962..9692
                     /locus_tag="PK460_01560"
     CDS             7962..9692
                     /locus_tag="PK460_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE53821.1"
                     /translation="MKKGLFKYFKVVWKEGILAIFFILFETVLETLVPFMASRVVDVG
                     IANNDLPYIYKWGALMVCSAFLAFFIGIISVRYVARTGQGFGAELRRAQFAKLQSLDQ
                     EARDKFAVSSLITRLTNDTYTIQNAIIIGMRGIFRAPFMITMVLMLSFNMNSKVAIVF
                     ALTLPVIAFAMLVIVFRVRPLYERMQRNFDELNRTLQENFQAIRVVKAYVTEEQEEAK
                     FKAINKMYRKVASNAFMTVSLNSPIMQLGLYSTIIGLLLVGGNLINTGEAKIGEIQGL
                     LTFVLQLLNAMLMLAQILIMFGRTSASKSRISEVLNTDTSLEYLINDLSVKAKPSLIV
                     KDVSFKYPGTSDDNYALKNINFTLKAGETLGIIGQTGSGKSTLVHLLARLYDVTDGEI
                     ILDDHNVKDYSADNLLDAINIVFQNNMLFSGTIRDNLLWGNKDASDEEINRALEASLS
                     DEIVNKLANGLNSEVGQAGSLLSGGQKQRIALARALLKRPKILVLDDSLSAIDTINEG
                     KIRTKLRKLYPQLTLIIVAQRVSAIKDADHILVLHEGEIHGAGTHNSLVENDEIYHDI
                     YTTQLKGAKA"
     gene            9689..11536
                     /locus_tag="PK460_01565"
     CDS             9689..11536
                     /locus_tag="PK460_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE53822.1"
                     /translation="MKKQSKPKHMKQTMRRLFSYLLKRPVPLVFIVLLVVWSGFTNGY
                     GIYVMREIIDKSILDFDINGLNKSLIKLAILFGSGALSALIYNPMMVKYSQTVIADIR
                     NELFAHSQKLPLSYFDRTPRGEIMNYFTNDVDTLNQALNTGFTTIIYSVVTGIGTIVF
                     LFLINFYLSLIVVFFIVVMFFMVLRNSQKMRVGFASVQKDTAHLNSYIEEMMLGLKVS
                     AMFSHQKEDMKEFKRRNDALTKSGIYANTLVLRKTPMIVSLSYFNYAISAIAGALFVL
                     NGWLSLGALGSYLIYVRQSSNPFNNFMTELNALYNGLSGAERIFAFLDLEQEIDEGKV
                     RLVSAVEIDGNIVETEHPTNSLAWKIPTANGEFTYEIFKGRVDFTNVVFGYKQDERVL
                     NNISLHAEKGEKVALVGSTGAGKTTIINLITRFYEIDSGTINYDGIDIRDIKKSDLRQ
                     SVSVVIQDTHLFTGTIFENIAYPSPGATIAEVERAAKIAHAHDFIVKLPQGYETMLYD
                     NGANLSSGQRQLIAIARAAILQPPVLILDEATSSIDTRSERLIEKAMDELMEDKTVFV
                     IAHRLSTIRNAKAILVMEKGSIIERGSHEDLILEGKVYYQLYTGQFEWT"
     gene            11691..13517
                     /locus_tag="PK460_01570"
     CDS             11691..13517
                     /locus_tag="PK460_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE53823.1"
                     /translation="MKRKKAKKHSILHLLKGHFWGMFFVTLLVIFHRVSYSFVPLFTQ
                     YILKYLQNNGVVTGVNLPLFVIEIFEKGQNVFEVSLYVALTLLLYQLFRFSLMVVEQR
                     LKGQVQESIALKLRVKLFDHIQSLSYHYHNNSDTGDLIQRVTTDVEVTTNFVVMRVMD
                     AVGLIFMVLSGAYQMYFLSPLLMYISLAVIPLYGISSYFYFKNIWKRYNDIEEKEAEI
                     LTVIQENVHNAKVVKAFANEKFETEKLEVKNKAHKNAVIKTNKIVGMYWAAMDFFALS
                     QYFVITLISVFLARNGQMDAGNAAAALMLVGLLIWPIRGLGRLINDVSHANVAYNRIT
                     TVLDEKSEFEINGDLLPEIAGKIEFKNVSFKFPGDSSYTLENLNFTIEKGQTVAIVGR
                     TGSGKTTIINLLMRMYEYEGSILIDDIELRDIEKHHLRKNMGTVLQNPFLYSRTIRDN
                     INITARKAPMEKIINASEIATIAHDIRDFEKGYDTMVGERGTTLSGGQKQRVAIARVL
                     ISEKPILIFDDALSAVDSRTDTLIRQALKQKEQTSTTLIITHRITTAKEADQIIVINN
                     KTVEDIGTHDTLAKAGGLYQVLWDIQGDLETEFLELVAKECV"
     gene            13521..15359
                     /locus_tag="PK460_01575"
     CDS             13521..15359
                     /locus_tag="PK460_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE53824.1"
                     /translation="MSADVFREEEAVKKVTFGVWKQILKIILRSKKELSLLLIFAVFM
                     SLAEASTIPLNAYAISNLIETYNESALLPFIVLSVTQIVVFAFGVFGFIYFGSKLETK
                     VRYTLRKESFKNLQKLPFSYYDSTKQGWIMARMTSDTNRLARIVSWGILDLTWSGFFM
                     FFTIVVLFISEWRLALVFVSFLPILLFIAIIFRKKVLILNRKSRFHNSQLTGLFNESF
                     LGAKTTKSLAIESELSDEFTKEARVMKKVTLKAILYGAIFSSFLLAGSYTIVAFVMAL
                     GGYFALQGLVTMPILFMFIRAAMNTFEPILNLTNFINELQVAQASAERVLQLVNEKPS
                     IDDTQEVKDVYGDLFDKKKENWEEIKGDVEFKDVTFYYNPKEIILDKFNLKVKAGQTV
                     ALVGHTGSGKTTIVNLFARFYEPRAGEILIDGKDYRKRSLSWLHSRLGYVLQMPELFS
                     TSIKENIRYGNLEASDEEVIAASKAVGLDEFVNTLEKGYDTHVGEAGNLLSVGQKQLI
                     SFARAIIADPRILILDEATSSIDSEAEAKIQKATDELLKGRTSFVVAHRLSTIVNSDL
                     IVVLENGKIIEQGTHAELLGNRGHYFELYRNQFIAERREEKYLEID"
     gene            15362..15565
                     /locus_tag="PK460_01580"
     CDS             15362..15565
                     /locus_tag="PK460_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOE53825.1"
                     /translation="MTRTYIVEGMKNERSVRRIERALFRVDNISYEINLEKNTVVIHF
                     SNRVDDDKVIGAISRAGYRAMRL"
     gene            15712..15834
                     /locus_tag="PK460_01585"
     CDS             15712..15834
                     /locus_tag="PK460_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53826.1"
                     /translation="MVLGSEKKYLCLLCGMIIDNPDYCEYCGATSENIVPYEEE"
     gene            15934..17205
                     /locus_tag="PK460_01590"
     CDS             15934..17205
                     /locus_tag="PK460_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOE53827.1"
                     /translation="MKIRFINALILTMEENRPLFYGEVHVEGNRITYVGPSGDFNEPF
                     DRIINAEGNLLMPGFKNAHTHSPMTFLRSYADDLPLYDWLHKAVFPLEDKLEPGDQYV
                     LSKIAFAEYLTSGVTACFDMYYSPLETAQAAIDVGFRAALVGTVTKYKESVCEMKAAY
                     HTINKMHPLVSYHLGFHAEYTSTPEILKELAKAAHELKVPIWSHISETETEVKECIER
                     HGYTPAVYFDKLGLYNYGGGGFHGVHFSDEDFAVYKKRGLSIVTNPSSNTKLASGIAP
                     INKFLKEGINVAIGTDGPASNNALDFFREMWLVTGLAKLREKDAAVVDAYDVLKMATV
                     NGAEAMGLKDAKYLSVGSLADIIMLDMQQPNMQPVNNIEKNIVYAGSKVNVLLTMIDG
                     KILYEDGEFNLDEPISEIYRKAQAITERLKS"
     gene            17208..17882
                     /locus_tag="PK460_01595"
     CDS             17208..17882
                     /locus_tag="PK460_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE53828.1"
                     /translation="MPNYNELKGNNLNFSIEKNKPNILKGKTLLFLGASFTYGHASFG
                     ESFVEYIEVRNDCTCIKEAVSGTTLVEHIEDSYITRLKKVPLGKKYDALLCQLSSNDV
                     RLKQEFGVIKESDYDTKTICGAIQYIAKYARDVLKCPVIFYTCPYFDKERYQKLVSIL
                     NEIATKMQFSVIDMYNDKNFNNISAETYALYMADPVHPTKAGYYFWLTPYIESHLFAF
                     FTKLIR"
     gene            17922..17996
                     /locus_tag="PK460_01600"
     tRNA            17922..17996
                     /locus_tag="PK460_01600"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17954..17956,aa:Met,seq:cat)
     gene            18439..18801
                     /locus_tag="PK460_01605"
     CDS             18439..18801
                     /locus_tag="PK460_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53829.1"
                     /translation="MEFKTVNYDSKIIKEGISHKGRRKSDDPVKIFSQQLKDQNVLYF
                     FYKDDECLYIGQTGICLWDRIIRHEDPEKDSKWFEEANKICLIILDKKVDVISRRNLE
                     STFIVNYLRAGHKLYNKE"
     gene            19079..20095
                     /locus_tag="PK460_01610"
     CDS             19079..20095
                     /locus_tag="PK460_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53830.1"
                     /translation="MRKSKYKPLADYLSASNKNSVSLTFNQIEKILGFKLPPSSRKYR
                     ANWSNNKMEALAWGWLPVGYESFDVRMSDEIANFRKVGNKLKSEKSKKSNRKQNSDLK
                     RQIENATDLIFLDVPNNLLKLEVDEYYMVKLTKTNSEIVEHELANDISYPQKAKKICE
                     ELIKRNNDFSSETFNIIIHRIAIENSTRSSKKTIDLLTDFALNSENQFLQRVKKGDLT
                     LVDDITDYLFKNNNRRDKSLASKVCRYLNEWLFDKDDFTINDSVVRKVLPYYLAYYKI
                     EKHYWANKNLDKLTYVEFFAIFEKIKEKLPELTRHELDHLLWYSYKNDNIRSTIAASL
                     AKHL"
     gene            20269..21273
                     /locus_tag="PK460_01615"
     CDS             20269..21273
                     /locus_tag="PK460_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE53831.1"
                     /translation="MKFERTLAICLDMNGCPNRCLHCWLGDLPNHKLNDKTATKIVKL
                     FKPYFENITFYSWLREPDFTKDYKARWLKDNKLSTIKPKRFELASYFTIIRDPEYVKW
                     LKAVGTKKVQLTFFGLEAMTDKYVGRKGAYKELIAATKILQDNDIDVRWQLFINEENK
                     DEVSHLLDVAAENKVTDIFVHEGSCDGNNRKLYDIRINKDNIPLKVKPYYLNYENLLS
                     ESECIELLKDDDSYFIPKHKDEIVIYITSDLNVYFNFTNPSAAWQIGNIEDDDISEIV
                     RKIVAGDVPALKFARSILLSELVKLYGDSNSDKVFSLEDYKMYLLNNYVDDKYNSKLQ
                     "
     gene            21419..21925
                     /locus_tag="PK460_01620"
     CDS             21419..21925
                     /locus_tag="PK460_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53832.1"
                     /translation="MQDTFNYNDYQNDQIVKKYDGRINRHHLGILFWRLGVAFSIIFI
                     VLLLARTLISITSIIIFMIGLVILSFIFLITLGTILIIDKNHGRHWKNLINILENRET
                     ALLVIDKILTFVPLICSVIIAFNIFALILLYTSKLPVRRFRLVTAIIIIILNIVALIY
                     ALAGGNFT"
     gene            21922..22428
                     /locus_tag="PK460_01625"
     CDS             21922..22428
                     /locus_tag="PK460_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53833.1"
                     /translation="MKTLKLRVLNPRMHNVIYMFDGKALKPKGDNMGHYVFNIETPAD
                     KVDILIIRRSPLRSRLWLVWQFLFFIVSLLGILDLQSKKLNKEAIYRATLYLSGEDEV
                     DLKFDTDNSSNAFVELTTTLQVEERENKTLSDPLIVRRAKVLKILKIITYIVLLITLI
                     IILILIKK"
     gene            complement(22527..24956)
                     /locus_tag="PK460_01630"
     CDS             complement(22527..24956)
                     /locus_tag="PK460_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HOE53834.1"
                     /translation="MNNKTKSRRLRFRNILLLWLVGLAGQLCWNLENQWFNTFVYAEV
                     GPFPQIVNAMVAVSAIATTFATFFFGTLSDRAGKRKPFISIGYIIWGIFTIAFGLTGL
                     LTKFTMNIWALATVIVLADTVMSFFGSMGNDAGFNAWTADLISDDNKGAIGTALAVQP
                     VIGTIIGTVLGGILIEAFGYIAFFSMMGGFVSLIGVLSLFLLKDSPKLEPSKRGSFWR
                     QFAEAFNFKYFFSQKELVLVNVVAAIYFIGFNVYFVHIGNLFISNYGFNAGDAGIIQG
                     IGLIIAILFTIPGSRLINRGKSPRVALISILMTIVGLIYLGLIGGYHDPANLFGAKNL
                     PLLLGVIVVGAGYILIMQTLGVWSKVLYPVGSKGQFEGLRIIFFVLIPMVLGSSISQP
                     IIRHFGIETVVEVLEDGTKVMGYIPTEALFYFASIIMVLALIPLIFLRKGYYKRMRNT
                     KISEAVSTAKEELGVDELYYTTPTPVFDKQKRHINFGYATDMSFVFDKKLVKKPLRLK
                     EWDFYQIQHGRYVVQLTLGHIAYAQNIAINLIDLEGEETYSASVVKLFKGAKSMTMPT
                     DPSVDNEIHYTDTKKKVDLHYVTKGRIRHLYGSMVNEKGVTFKVDVKLERLENHDAMV
                     INTPFEDSLYHFYLNYKIGNLRASGTFSMDDLAFTFDPKVDFGLLDWGRGVWPFHEVW
                     YWGQVNDYLADGRMINLNLGYGFGNLEAASENMMIIDGKAYKLGRLYIENDVTLDYMA
                     DWIIKSDDGKIDLVMKTIYDRYSDNDFKVIFMKCHQVYGRYYGTITLDDGTVVELTGQ
                     QGFFERSHNKW"
     gene            complement(25072..25872)
                     /locus_tag="PK460_01635"
     CDS             complement(25072..25872)
                     /locus_tag="PK460_01635"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOE53835.1"
                     /translation="MFNLMHELFKDKDTGVFTFSAFSLCHIIYLLLIIGGIILVIFLY
                     KNKTQEAKTKLITTTVTIAFALYIADFFLMPFATGTIDIGKLPFHLCTLMSVMCFVVR
                     HNKKMEVFKTAFTLLGLIGGLMYITYPAGVAQADGYSYRIVQTVIYHGLMIAQGVFAL
                     VFDDLDLSWKNFKYDVIAVLAVAVWAYIGNTLYSGSNFWLSYEHDFNWFFVKHDGLYL
                     IDDEKDAFIAPLMSVSSVLAISALLRLIAIKLKSLFVKEQVLEAEKEA"
     gene            complement(25926..26723)
                     /locus_tag="PK460_01640"
     CDS             complement(25926..26723)
                     /locus_tag="PK460_01640"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOE53836.1"
                     /translation="MTAFMKLSIFNLICLIVIGVLLFLCKKFIKERRTHDLILKTTSI
                     LLILIHYSAVYVEFFQNQGVAYASASYILPVYPCHIMMWLLVIVAFTRHKKSRFFEGL
                     AEFVFVVGTLAALIGALFNMNFLNSSNPTFTNYFLFKGVFGHMVMFFGTTYLFVFEYV
                     KPRVEKTMISVIVGLLFFVADGIIINTLYAKFDIPPVNAMFLLEPPFEDYPFINFFTI
                     GSIGLILCFIGLLIYEHITLPKAEQWITKISTKSNKQDEDLTTDKTN"
     gene            complement(26840..26915)
                     /locus_tag="PK460_01645"
     tRNA            complement(26840..26915)
                     /locus_tag="PK460_01645"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26880..26882),aa:Phe,seq:gaa)
     gene            complement(26920..26995)
                     /locus_tag="PK460_01650"
     tRNA            complement(26920..26995)
                     /locus_tag="PK460_01650"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26960..26962),aa:Asp,seq:gtc)
     gene            27257..28948
                     /locus_tag="PK460_01655"
     CDS             27257..28948
                     /locus_tag="PK460_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE53837.1"
                     /translation="MNNKVPLSEYPRMIMVRDSYLSLNGVWEYAIRENDERVPIEFDG
                     DILVPFSPETPLSGVNRTVRPNDVLFYRKRFSLPKGFKKEYVTLHFGAVDQVVTVYLN
                     GTKIGFNQSGFHPFSIEISKVLKIDNELVLVVRDYSDTRFYARGKQKIKRGQIWYTPQ
                     SGIFMPVWLESMPRDYIEKLKITPRYDESLVEITVFTNSKDNDAVIIFDGKEYTVKPN
                     KVYKLKVKDFISWSPDNPHLYPFKVKYCEDEVDSYFAMRKISVDTDEEGIKRIYLNNK
                     ILFNTGLLDQGYYHEGYLTPPDEATVINEIKVAKEMGFNVLRKHIKIEWERFYYHCDR
                     LGMLVWQDFVNGGEPYNFVTVHVPAVLNIHFKDTNYKRFARQNALGRELFYAEAKTTI
                     EHLYNYPSIVLWTIFNEGWGQFDSHKLLPFVLELDNTRLIDVNSGWHDMGLGQFRSHH
                     VYFRKYRFKKDKLDRCVILSEFGGYAHRIPGHYYGVANFGYRKYNTKEELEKAVINLY
                     EKEIIPAVKQGLAAAIYTQLSDVEDELNGVLTYDRKVVKIDPKVMRELNEKLLKA"
     gene            29118..>29264
                     /locus_tag="PK460_01660"
     CDS             29118..>29264
                     /locus_tag="PK460_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE53838.1"
                     /translation="MYCEKLITLRRNRGLTQQQLADALMLSQSMISKIERGITRLDPM
                     LAIRF"
BASE COUNT         9974 a         4652 c         5428 g         9210 t
ORIGIN      
        1 tataacaaga ggtttgatga aatatcagac ctttttttgt taatagaaat gtaaataggg
       61 ttgttttata tctttttttg gcaaaatttt ggcatttatt atgctgttcc tatgggcata
      121 aagatatttg ctatcttgtt aatggtgaat taaaatagaa aagtgaaatt gaaaatattt
      181 attattttag taatattaat caccgtgttc gcattagttt gataaaaagg gggaatcaaa
      241 aagatgaaca aaaatcgaaa atggttagct aggagcgccg ttatttcgct attaattagt
      301 gcaattatca caccagcgtt ttttcgtaag gacgcggtaa aaacaaaagc ggcaacgacg
      361 ataccggtct tagtcggcga ttcaattgga tgggaaccat cagtgtcata aaaattcacg
      421 gaatatcccg gcggttctga tatttatgta tataccttta cgcggtcgca cgctggatat
      481 tacaaaatat atgactataa tactggggca ccaatggaag accttcaatt tgataccaca
      541 ccatcgcttt cacgcgatta tatttatcga tatgaagatt cgcccgatgc taactttatt
      601 acaatttatc ccggagaaat taaggttcaa gtttttaatc ctgataacgc tgggggtagt
      661 cctgcgggcg caaggcggat gaaagtcgag tatacaagtt tactcggggc aacaccatca
      721 acaccaacag gtacatcttc gtatgcattg cggggccctg ctgttggtga ctggacaacg
      781 cacgatatta ataattgttt agttggtatc agtggttatg atggccgtta tctttcactt
      841 actagagcct ttaacgctga tgattttaaa atctttgtgt acaaacaaac attaaatgaa
      901 ccttattaac gcggatttat tgattttgat gatcataagt cgaatgcaag tgatgtaatt
      961 atgcctggtg atccttcttc aggttatcat gagagttttc atgtaaatac tgctggaaat
     1021 tatatggtcg tttatgatct taaaatgcag aaaatttact ttcacaatgc tggtacaacg
     1081 tatcataaag taacaaaata ttatggcgaa cttttaattg aagaagcagc agcctttact
     1141 ggtttaaatt ataccccggc accattttat caaccagaga aaaaattcgt tggttggtat
     1201 gaagacgctt tattagaaac ggcatatgtt ccaaaagatt taacaagtga cattacattg
     1261 tacgctaaat ttgtggattc accgagtgtt aatttaaggt tttacgatcg taatttagat
     1321 attaaagtgg ataacggcgg atttaacgag tatgttgtat ttgcttatgg taagaataca
     1381 gacggaactg aaccattagg aggttggcca ggccggatga tgcaaaaagt agtagatatg
     1441 ggacatgaat tatcttacgg ctattatgca cttaatattt caggacaaga tagaccagaa
     1501 aaagttactt ttaataatgg atgtcaacct ttcgaaccag agcatgtttc aaccccggaa
     1561 cttgtgttac ccgctgataa gaatctctat gaacacgatg tagcagcgta gagtgtaatg
     1621 actccagttc atgacgctgc aacaagttac gccatcaatt ttctctatga aacaagaaac
     1681 tgtgacgcaa ctggggcggt taataatatc tcaagtcata aacgggagga actcggaaat
     1741 ttatttaatg aattagcgcc agatgtgcaa gcggacttaa aaaatgcgac tgcaacatta
     1801 agtggtgact tagtgtcaca agctatggcg cgttatgtat atattttaca aaaatatgga
     1861 acggttacat ttacaaactt cattgaccgt acactaccac caagcggtat acccgcagtt
     1921 cgtggtgata ataaacaaaa tttctatgta ctaggcataa tggcctttat cgctgtggcg
     1981 ggttttttct tcctaaatcg taagcgtcgt tactaataag tttaatagtt attgtataaa
     2041 tttaaaaata taaaaaagag cggttatcat cattttatga agatgtaccg ctcctttaag
     2101 aatgttatcg tgacagttag ataacaagtg atgcgctatt caagtgcgga ccacgtaatt
     2161 gtttcagatt tgtaaatttg ttttaattga tcgcgtgtga tgtcactaag gacatggttt
     2221 ttattgctga cgacagcaac gatagcgtcc ttactaatta cgccgtagct accagcaagt
     2281 gcgggttcag tttcattaat atctaaatcc cgggctaaaa aaccaatatg aagtggttca
     2341 gcgctatctt tattactccc ttgtgttgct tcataagcca tttcattact agtgtagtta
     2401 tgaactgctt taaaggcggg cactaaagaa gtaaaagcac tacttaaggc acgactaata
     2461 ctttcgacag attttgaacc accaagttta atgtggattg gtgcttcatt agtgttataa
     2521 agcattttcg ctagagcgct attttcgctt ttcgctaaga gtgttgtaaa actttcagcc
     2581 tcttcaatat tttgggtaac gattccgtct ttggccttaa taatacttaa gccttcgcgt
     2641 gacttactaa aggcgatgaa ccctgtaatt agggcatatt ctttttcact taagtcacta
     2701 ggagcttttg tgatgtagtt aaagtttcgt gataatttgt acgaatcatc aataattgat
     2761 ttttcggaga cgcgaacccc ttcaatcatt aaagctttaa gctcggaaga ttcaagtgcg
     2821 gcgtaggaaa tatatccaat accgtataaa tcgttattga gtaaagagat catatcgcta
     2881 tcatccgcag ttagtgtagc tcctttaata atagtgttat cactaatagc ggctttaaaa
     2941 tcaatcgtgg taaaaaaact gtcacgtatg gtggaagaag tttcccgtgt ataacgggta
     3001 atagtgttac ttgtatcaaa tggctcttcc ttaatccgca tggatacaag ctcgagacta
     3061 cccatagcaa tcgccataag ttctccgatt ctgcaaattg catttttcat atctgaacac
     3121 ctcctttcag acacataaac tttaaagcaa acggagagct agatatatca taaagctgta
     3181 aaaagttgta aagaagttgt aaaagtggta gtttttcgct gatgcttttg gttaaaatat
     3241 cattaaagta atagttttat tactaaaaaa cttaaatttt cccgcaaaat ttttaatttt
     3301 acagttaatt taggtttgtt ttaagtgtgc ctttctcaca aaaaagtgac aaattaatag
     3361 ttaaagatat aaaaaacaat aaagcgaaat ctattttttg attcagttca tactcctatt
     3421 gataaacctt cacctgatcg tcgcgcatgt atatttaatg tttactttgc agatgatatg
     3481 ttaattgttg attataagta ataagatact tgtacagtta cgatacttta tttattagct
     3541 gcatttttta ccacaaatat aactggcaaa ataccacaaa agtaaacaac aaaacaccac
     3601 aaaatatagt aattactttt taaaacaatc gtgtaacgca taattttatg tgaaaaccat
     3661 ttgtgctctt aaggcataaa caaagtacaa aaatagtttt ataatgtttg aagtggaaga
     3721 gattataatc aaaaagtaaa aaagtcttta atttataaaa agtcttatat tatatttgct
     3781 aaaataaaga taattgaaaa gaggagctac ttattatgag tccactagaa attgtgttag
     3841 taagtgtctt aggcgcactt attttaattg ttgccattta tttatttgtc gtacacacaa
     3901 atgcagttaa tcgcttagaa atatttgaag aagttaacaa agaagccaaa agcggaaaga
     3961 ttgctttttt aggtgatagt ttaacagact tttttccgat tcaagatttc tttccgggtg
     4021 taaagatata taaccgcggt attgctggtg atacgaccaa aaatgtatta aaaaggcttg
     4081 ataatgtagt agcactagcg ccgcgcaagt tatttttaca aattggcacg aatgacttag
     4141 ccaaaggaag tagtcctcgt aaaatagtcg ctaatataaa aaagattatt acaacattca
     4201 aaaaagaagt accaaatatt gaagtaattt gcatttcact ttatcccgta tcacgcacta
     4261 aaaagttttt atccacattt attgttggat taagaacgaa taagaaaatt aaaaagacaa
     4321 atgaactctt aattaatttt tgtgctgaag aaggacttaa atatttagat ttctacactc
     4381 ttttagcgga taaaaaaggg cggattgacc gccgttatac agttgaaggg ttgcatattt
     4441 caggtaaggg ctatgcggta attgcaaagg ccttagcccc ttatttataa attaacgata
     4501 aggaataatt aatccgcgga aatgttcaac gataaagtca ccgatttcat cactatataa
     4561 tactttcatt agtgcgcgta ttctatcatt attttcttcg ccgcgacgaa taccgacaat
     4621 aaatgcataa tcattactag tttcagtagc gagaatatgt tcataactaa gtccgctgtt
     4681 ataagcttgt gaaccagtca taatgtaaaa agcttcttca ggatgctttt gggcttccgg
     4741 aattaaatct ggattcatat attcaatggt caaaaagtca agtggtgttt gtctgtaatt
     4801 tggtgttttt tcttgttcgg taaatacagt aacagtaaaa acttcctcga taaaactcat
     4861 aatccgctca taatcttcag cgcgatcact aatgacaatt ttgtcgccag gattataatc
     4921 ctcgagtgtt gttttcgttc ctgaatatac accaagtgct gaaagataga cgcgaccagc
     4981 actagtaagc tctgcgcgtt ttgatacccg gttattatag gcaattaaat catctgttct
     5041 ttgaataata tttgcgtcaa tcccgccact tgataatgaa gaattagcga gcaaagcatt
     5101 atcaattgtc tcaagattta agcgataacc catttcctta agtgaagttt tagccgcgtt
     5161 taaaatctct tcatgaatgc aacctctcgt tgcgacacga attagtttct cagtcttaca
     5221 accgactaag gataagagca ttactaacgg gataacaatt tttttcataa gctttaaggt
     5281 tagtgtgcga gatacatttt aatgattact ttcgcttata atctaccttt attaaaaggt
     5341 atttcatctt aattaaagca tatttgcagg gaattacaac caaaaacacc taaaaatacg
     5401 aaaaaaatga taacgataac aattttttag ccaatttagc gacttttctt agtaaataat
     5461 gcgttttctt tactaaagaa agaagtagtg taatgtgata tcatagtgta agcaggaggt
     5521 agttatggtc aaacaacaaa ataatttatt tctcttaaac aacttattca atgtgcgtga
     5581 cttaggtgag attgatacaa agtatggtac taagacacgg cgttataaat atattagagg
     5641 tactgccaaa ggcacaatga ctgaaatcga aaaagagcac ttttataact taggagtaag
     5701 agtagtagtg gatttacgct atacgaatga aatcgaaaaa acaccaagtc cgttacgagg
     5761 ttataaagat atcaagtatt atcatgtcga tatgatgggc gaattttggc aaatgcgtga
     5821 acaaggttat aaagatatga ccgacttata cattgatttg cttgatgact cgcaagagaa
     5881 actgctaacg atctttaaaa tcttcttaaa gtatccgcgg gacggtattt atttccactg
     5941 taccgctggt aaagatcgga ccggagttgt cacaatgtta ctcctagact tagtgggtgt
     6001 tccgcattct gtaatcgttg aaaattatag tgaatcgtac actaataact tatcgcggcc
     6061 aggctataaa tttattaaac cagaatggcg acgcttcatt cttagtgaac ctgaatacat
     6121 ggaacgaaca ttagatcatt tatacacaaa gtatggtggg agcagagctt acttatcgca
     6181 tattggctta cgtgatgatg aaattaataa tctcgcggcc acaattgtcg aataacattg
     6241 caaaaagcac ctaaaataag tacaattatt gtgtaaacac cgctataagc aaaaggagat
     6301 acatatgaaa accttagcaa acttactcga taatgataca gtaacgattg tattattttt
     6361 tgctttccta ctcttagcgg tcgttattta tcttattgtt tccaaaaaag aaccaagtaa
     6421 agaaaaaatg cggcctgaag aaagagcgct aaaaatggtt gaagcgccta aaacagaagt
     6481 taaaacagaa gtaacgccag aaaaaccggt tactgaagaa gttaaaacaa aaaaagtaga
     6541 aagagaagca caaaaagtta aagttaaaaa agaagacact gatgacgatc aattcgccgc
     6601 cattaaatat agttattctt atactgcaca agttagttta gcgccagaag caagtcgcga
     6661 acgctattta aaaataaaac aagaaattct ctcttacact agtgttaata atactatttc
     6721 atggcgtgaa gaacgcttta cattaagcgg gaaaacaatt gttaaattta aattgattgg
     6781 taatgtttta cggatgtata ttgccttaac gcttgaagaa ttaagtgatg taaatattaa
     6841 gattgatgat gttagtcgtt ataaagaaca cgaattaaca ccaagtttat tacgtatcag
     6901 tggtaatact ggtgtcaagc gtgcgctcca agcgattgat attttaatgc agaaacttga
     6961 actaaaaaaa gacccacaat ataaactagg ggcaagtgat ataccagcag cgcaaacaat
     7021 cgatgaactc ttagaagctg gacttattcg gaaaatttaa tttatgaaga agatagcctt
     7081 agttttaggt ggtggtggcg cgaaaggctc ctatcaaatt ggtgtattaa aagcgctgca
     7141 cgagactaaa atattaaaac gcgttagggt cgctagcggt acctcgattg gggccatcca
     7201 caccctttta ttaatgcatg atttaagtat tgaagagatg attaatgtgt ggaaagaatt
     7261 cacaaatgaa gcgatttatg gtagaaacaa attcaaagaa acatggaata taagcggctt
     7321 ttataacact aaaggtatct tagaaaaaat attaccgttc actaatgaag aagcatttag
     7381 aaatacaaag attgaaggct atgtcacttt agcaaaagta cccactaaag gtttatttgc
     7441 aaaattaaat atgaaaaatt acgaaaaaga agttgtcttt ttaaatgaga gtgaacaccc
     7501 gctaaaagct gttttagcaa gtgctgctat tccgcttgtc tttggggaaa aagaaattga
     7561 aggcactcgt tatgtggatg gcggtttagt cgataatttc ccgtttacac cagcgcttat
     7621 cgctaacgct aatataatta tttcaattgg tctagatcca aaacttttac caacaaaacc
     7681 attacccgga cgcttacata ttgattttac cccgctagaa gagttaggac catttcctaa
     7741 agtgtcgctt gattttagtc ctgaaaaaac acagcaatat tttgatttag ggtatgagaa
     7801 tgctaaggcg ttgatagcgt acttaaaacg cgaaaagttt ttctggtttg gtggaagact
     7861 aaagctaaga aaaggacgcc tagtaacgtt agaaacatta acagcaaaaa ctaagtagct
     7921 tatatataga gaaaaagaga ttttactggc ataataatgc tatgaaaaaa ggattattca
     7981 aatattttaa agtagtatgg aaagaaggga ttcttgccat cttttttatt ttatttgaaa
     8041 ctgttttaga aacactagtg ccatttatgg caagtcgcgt tgttgatgtt ggtatcgcta
     8101 ataatgattt accttatatc tataaatggg gtgcccttat ggtatgttcg gcttttcttg
     8161 ctttctttat tggcatcatt agtgtccgtt atgtcgctcg tactggtcaa ggatttgggg
     8221 cggaattacg tcgtgctcaa tttgcgaaac tacaaagttt agaccaagaa gcgcgtgata
     8281 aattcgcagt gtcttcctta attactcggc ttacaaatga tacttacact attcaaaatg
     8341 ccatcattat tgggatgcgc gggatattcc gtgctccatt tatgattacg atggtattaa
     8401 tgcttagttt caatatgaat tctaaagtag caattgtatt tgcacttacg cttcctgtta
     8461 ttgcgtttgc gatgctagtc attgtttttc gagttagacc actttatgaa cggatgcaac
     8521 gtaattttga tgaattaaac cgtactttac aagaaaactt tcaagcgatc cgtgttgtta
     8581 aagcttatgt tacggaagag caagaagaag caaagtttaa ggcgattaat aagatgtatc
     8641 gtaaagtcgc tagtaatgcg tttatgacag tatcactcaa tagtccgatt atgcaacttg
     8701 gactttattc aacgattatt ggtcttctac ttgtcggtgg taatttaatt aatactggcg
     8761 aagctaaaat tggtgaaatt caagggttac taacctttgt tttacaactc ttaaatgcga
     8821 tgctgatgct tgcgcaaata ttaattatgt ttggtcgcac tagcgcttca aaaagtagaa
     8881 ttagtgaagt acttaacact gacactagtc ttgaatactt gattaatgat ttaagtgtta
     8941 aagcaaaacc gagtttaatt gtgaaagatg taagttttaa gtatccgggc acaagtgatg
     9001 ataattatgc tttaaaaaac attaatttca cgcttaaggc tggtgaaaca cttgggatta
     9061 ttggtcaaac tggaagcggt aaatcaacac tcgtccatct tttagcaagg ctttatgatg
     9121 taactgatgg tgagattatt ttagacgacc ataatgttaa agattatagt gcggataatc
     9181 ttctagatgc tattaacatt gtctttcaaa ataatatgct ctttagcgga acaattcgtg
     9241 ataacttgct atggggtaat aaagatgcaa gtgacgaaga aattaatcgg gcattagaag
     9301 caagtctaag tgacgaaatt gtgaataaat tagcaaatgg tttaaatagt gaagtcgggc
     9361 aagcaggctc tttgctttca ggtggtcaaa aacaacgaat agcattagca agagcattac
     9421 taaaaagacc aaaaatatta gtgcttgatg attctttatc agcgattgat acgattaatg
     9481 aagggaaaat tcgcactaaa ttacggaaac tttatccgca acttacttta attattgttg
     9541 cgcaacgtgt gagtgctatt aaagacgctg atcatatttt agtgttgcat gaaggggaaa
     9601 ttcacggggc aggtacgcat aactcacttg tggaaaatga tgagatttat cacgatattt
     9661 acacgaccca actaaaagga gcaaaagcat gaaaaagcaa agtaagccta aacatatgaa
     9721 gcaaacaatg cgccggctct ttagttattt attaaaacga cctgtgccgc tagtttttat
     9781 tgtactcctt gttgtgtgga gcgggtttac taatggttac ggtatttatg taatgcgtga
     9841 aatcatcgat aaaagcatat tagactttga tataaatggt ttaaataagt cacttattaa
     9901 attagcgatc cttttcggtt caggtgcttt aagtgcactt atttataacc cgatgatggt
     9961 taaatattca caaactgtta tcgccgacat tcgcaatgaa cttttcgctc atagtcaaaa
    10021 attaccgcta tcttattttg acaggacacc gcgcggtgaa attatgaact atttcactaa
    10081 tgatgttgac acacttaatc aagcacttaa tactgggttt acaacgataa tttattcagt
    10141 agtaacaggc attggcacaa ttgtatttct tttccttatt aatttttatt tgtcactgat
    10201 tgttgtcttt ttcatcgttg taatgttttt tatggtactg cgtaatagtc aaaaaatgcg
    10261 ggttggtttt gctagtgttc aaaaagatac tgcgcattta aatagttata ttgaagaaat
    10321 gatgttaggg cttaaagtat cagcaatgtt ttcacaccaa aaagaagaca tgaaagaatt
    10381 taagcggcgc aatgatgcat taacgaaaag cggaatatac gcgaatactt tagtgttacg
    10441 aaagacacca atgattgtta gtttgtcgta ttttaattat gcgattagtg cgattgctgg
    10501 tgcgcttttt gtattaaatg gctggctaag tttaggagca ctaggatcat acttaattta
    10561 tgtccgccaa agttcaaatc cgtttaataa ctttatgact gaattaaatg ctctctataa
    10621 cgggctttca ggggcagaac ggatttttgc ttttcttgac ttagaacaag aaatagatga
    10681 aggtaaagtc cggcttgtca gcgctgttga aattgatggt aatatcgttg aaactgaaca
    10741 cccgactaat agtttagcgt ggaagattcc gacggctaac ggggaattca cctatgaaat
    10801 atttaaaggg cgagtcgatt ttactaatgt tgtttttggt tataagcaag atgaacgcgt
    10861 cttaaataat attagtttac atgccgaaaa aggtgaaaaa gttgcgcttg ttggttcaac
    10921 tggtgccggt aaaacaacaa ttatcaatct tattactcgc ttttatgaaa ttgatagcgg
    10981 cacgattaat tatgatggga ttgatattcg cgatattaaa aaaagtgatt tacgccaatc
    11041 agtaagtgtc gttattcaag atacacattt atttactggt actatttttg aaaatattgc
    11101 ttatcctagt cctggcgcaa caattgccga agtagaacgc gccgctaaaa ttgcgcacgc
    11161 tcatgatttt attgttaaat taccacaagg ttatgaaacg atgctttacg ataacggggc
    11221 aaatctttca agtggacagc ggcaacttat tgctattgcg cgcgctgcta ttttacaacc
    11281 accagtctta atccttgatg aagctactag ctcaattgat acccgtagtg aaagactcat
    11341 tgaaaaagcg atggatgagt taatggaaga taaaacggta tttgttattg ctcaccgttt
    11401 gagcactatt cgaaacgcta aagcaatatt agtaatggaa aaaggctcaa ttattgaacg
    11461 cggaagtcat gaggatttaa ttttagaagg taaagtttac tatcagttat acactggtca
    11521 atttgaatgg acatagaata aacttcctta tgaaagcact ttactaggta caatttgtgt
    11581 aaagtatgta aatatgtacc ttttttaatt ttatcgttgt tataaattat tccatttaat
    11641 gtataatagt agagattgtt ttaatagaat ctattaaaag aaggatagat atgaagagaa
    11701 aaaaagcaaa gaaacattca atcttacact tactaaaagg ccacttttgg gggatgtttt
    11761 ttgttacgct tttagttatc ttccatcgtg tatcgtattc ctttgtacca ctatttacac
    11821 agtacatttt aaaatactta caaaataatg gagtagtgac cggtgttaat ttaccgttat
    11881 ttgttattga aatatttgaa aagggtcaaa atgtttttga ggtttcactt tatgttgccc
    11941 ttacgctcct tttatatcaa ttatttcgtt ttagtttaat ggttgttgag caacgactta
    12001 aaggccaagt gcaagagagc attgctttaa aattacgcgt taaacttttt gatcatattc
    12061 agtcacttag ctatcattat cataataata gtgacactgg tgacctcatt caaagagtaa
    12121 cgactgatgt ggaagtgaca acaaactttg tcgtaatgcg cgtaatggat gcggtcggtt
    12181 taatattcat ggtgttaagc ggggcgtatc aaatgtattt tttaagtccg cttttaatgt
    12241 atatttcgct ggcggtcatt ccgctttatg gtatttcttc ttatttctat tttaaaaata
    12301 tttggaagcg ttataacgat attgaagaaa aagaagcgga aattttaacc gttattcaag
    12361 aaaatgtcca taacgctaaa gttgtgaagg cttttgctaa tgaaaagttt gaaacagaaa
    12421 agttagaagt taaaaataaa gcacataaaa atgcagtgat taaaactaat aaaattgttg
    12481 gtatgtattg ggcagcaatg gatttctttg ctcttagtca atattttgta attaccctca
    12541 ttagtgtgtt tcttgctaga aacggacaaa tggatgctgg taacgcagcc gcagcgttaa
    12601 tgcttgttgg ccttttaatc tggccaattc gtggtttagg ccgattaatt aatgatgtct
    12661 cgcacgctaa tgtagcatat aaccgtatta cgacagtact cgatgaaaag agtgaatttg
    12721 aaattaatgg tgatttatta ccggagattg cgggaaaaat tgaatttaaa aatgtgtcct
    12781 ttaagtttcc tggtgatagt agttatacct tagaaaactt gaactttaca attgagaagg
    12841 gccaaactgt agcgatcgtt gggcgcactg gcagcgggaa aacaacgatt attaatttat
    12901 taatgcggat gtatgaatat gaagggtcaa ttttaattga tgatattgaa cttcgcgaca
    12961 ttgaaaaaca tcacttacgg aaaaatatgg gtacggtttt acaaaatccg tttctttact
    13021 cccgcactat tcgtgacaat attaatatta ctgcgcgtaa ggccccgatg gaaaagatta
    13081 ttaatgccag tgaaattgcc acaattgccc atgatattcg tgattttgaa aaaggttatg
    13141 atacgatggt tggcgaaaga ggaacaacgc tttctggcgg gcaaaaacag cgggtagcga
    13201 ttgcgcgcgt gcttattagt gaaaaaccaa ttttaatatt tgatgatgct ttaagcgcgg
    13261 ttgatagtcg caccgatacc cttattcgtc aagcattaaa acaaaaagaa caaacatcga
    13321 caacgcttat tattactcac cggataacta ccgctaaaga ggctgatcaa attattgtaa
    13381 ttaataacaa gactgttgaa gatattggaa cccatgacac cctagcgaaa gcgggcgggt
    13441 tatatcaagt cttatgggat attcaaggag atttagaaac agagttctta gaacttgttg
    13501 ctaaggagtg tgtttagagt atgagtgcag atgtttttcg cgaagaagaa gcagttaaaa
    13561 aagtaacttt cggagtttgg aaacaaatct taaagattat tttaagaagt aaaaaagaac
    13621 tatcgttact cttaattttt gcggtgttta tgtcactcgc ggaagcttca acaattccgc
    13681 ttaatgctta tgcaattagt aatcttattg aaacatataa tgaaagtgcc ttactcccct
    13741 ttattgtatt aagtgttacc caaattgttg tctttgcctt tggtgtcttt ggctttattt
    13801 actttggttc aaaacttgaa acaaaagtac gatatacatt acgcaaagag tcatttaaaa
    13861 atttacaaaa attaccattt agctattatg actcgacaaa acagggctgg attatggcca
    13921 ggatgacaag tgatacaaac cgtttagcgc gcattgtctc atggggaatc ttagatttaa
    13981 cttggtcagg tttctttatg tttttcacga ttgttgtctt atttattagt gaatggcggc
    14041 tagctttagt gtttgtgtca tttttaccaa tcttactttt tattgcgatt attttccgta
    14101 agaaagtttt aatcttaaat cgtaaatcac gtttccataa tagtcagtta actggccttt
    14161 ttaatgaatc atttttaggc gctaaaacaa ctaagagctt agcaattgaa agtgaattaa
    14221 gtgacgaatt tacgaaagaa gcgcgcgtaa tgaaaaaagt gacgttaaag gcgattcttt
    14281 atggcgctat tttctcgagt ttccttttag cgggcagtta cacaattgtt gcctttgtca
    14341 tggcgcttgg tgggtacttt gccctccaag gcttagtgac gatgccgatt ctctttatgt
    14401 ttattcgtgc ggcaatgaat acttttgaac ctatcttaaa cttaacgaac tttattaatg
    14461 aactacaagt cgcgcaagct agtgctgaac gtgttttaca attagtcaac gaaaagccgt
    14521 cgattgatga tacgcaagaa gttaaagatg tgtatggtga tttatttgat aagaaaaaag
    14581 aaaattggga agaaattaaa ggtgatgttg aatttaaaga tgtgacattc tattacaatc
    14641 caaaagaaat tattctcgat aaatttaatt taaaagttaa agctgggcaa acagtagcac
    14701 ttgttggtca taccggcagc gggaaaacaa cgattgttaa tcttttcgca agattctacg
    14761 agccgcgcgc tggtgaaata ttaattgatg gtaaagatta tcgaaagcgt agtctatcat
    14821 ggctccatag ccgcttagga tatgttttac aaatgcccga gcttttctcc acatcgatta
    14881 aagaaaacat tcgctatggt aatttagagg caagtgatga agaagtcatt gctgcgagta
    14941 aagcagtcgg ccttgatgaa tttgtgaata ccttagaaaa gggatacgac acgcatgttg
    15001 gtgaagctgg taacctttta tcagtgggcc aaaagcaact catctctttt gcgcgagcaa
    15061 ttattgctga tccacgaatt ttaattcttg acgaagcgac tagttcgatt gatagcgaag
    15121 ctgaagcaaa aatccaaaaa gccactgatg aactcttaaa aggccgaaca agttttgttg
    15181 ttgcgcaccg cttaagcacg attgttaata gtgatttaat cgttgtttta gaaaacggta
    15241 agattattga gcaaggtact cacgctgaat tactaggtaa tcgcgggcat tactttgaat
    15301 tatatcgaaa tcaatttatc gcggaacgcc gtgaagaaaa atatctggag attgattaat
    15361 agtgacacgg acttatattg ttgaagggat gaaaaacgaa cgatccgtac gtcgtattga
    15421 acgcgcgtta tttagagtgg ataatataag ttacgaaata aatttagaaa aaaatactgt
    15481 tgtgatccac tttagtaata gagtagatga tgacaaagtt atcggtgcaa ttagtcgcgc
    15541 tgggtatcgg gcaatgcgat tataattaat gaaaatttaa aattttgtaa taaaaaactt
    15601 gatttaaaca catttataaa taaagaaaat tagttgtaaa ttaatgcatc agcgggttta
    15661 cttctaataa ttataagata taatagggta tgggaggaag aggaaataaa tatggtatta
    15721 ggttctgaaa agaaatattt atgtttatta tgtgggatga ttattgataa cccagattat
    15781 tgtgaatact gtggtgcaac atcagaaaac atcgttcctt acgaagaaga ataaatatta
    15841 aataaacata taaaaaagca tggggcaacc catgtttttt attattaata aaaacatgct
    15901 aaaattaagt atctaataaa tgaggaaaac attatgaaaa tacgttttat taacgcactt
    15961 attcttacaa tggaagaaaa tcgtccgctt ttttatggtg aagtccatgt tgaaggtaat
    16021 agaataactt atgttgggcc aagtggtgac tttaatgaac cgtttgaccg tattattaac
    16081 gctgaaggta atttactcat gcctggcttt aaaaacgccc atacgcattc accgatgaca
    16141 tttttacgga gttacgctga tgacttaccg ctttatgact ggttacataa ggccgtattt
    16201 ccgcttgaag ataaacttga gcctggcgat caatatgtct taagtaaaat tgcttttgcg
    16261 gaatacttaa caagtggcgt gacggcatgt tttgatatgt attattcacc acttgaaaca
    16321 gcgcaagcgg caattgatgt tggttttaga gcggcacttg tgggcactgt tacaaaatac
    16381 aaagaaagtg tttgcgaaat gaaagcagca tatcacacga ttaataaaat gcatccgctt
    16441 gttagttatc atcttgggtt ccacgctgaa tatacaagca cgcccgaaat attaaaagaa
    16501 ttagcgaaag ctgctcacga attaaaagtc ccaatttgga gtcatattag tgaaacagaa
    16561 acggaagtaa aagaatgtat tgaacgccac ggctacaccc cagcagtgta ttttgataaa
    16621 ttaggtttat ataattacgg tggtggcggt ttccacggag tgcactttag tgatgaagac
    16681 tttgccgtgt ataaaaagcg cggtttaagt attgtgacca atcctagttc aaatactaaa
    16741 cttgcaagcg gaattgcccc gattaacaaa ttccttaaag aagggattaa tgttgcaatt
    16801 ggcactgatg ggcccgctag taataacgcg cttgatttct tccgtgaaat gtggcttgtt
    16861 actggtttag cgaagttacg tgaaaaagac gcggcggttg ttgatgctta tgatgtcctt
    16921 aaaatggcta ctgtaaatgg tgctgaagca atgggattaa aagacgctaa atatttaagt
    16981 gttggcagtc tagcggacat cattatgctt gatatgcagc aaccgaatat gcaacctgtt
    17041 aataatattg aaaaaaatat tgtgtacgct ggtagtaagg ttaatgtatt attaacgatg
    17101 attgatggca agattttata tgaagacggt gaatttaatc ttgatgaacc tattagtgaa
    17161 atttatcgta aagcgcaagc tattactgaa cggttaaaaa gttaaatatg ccaaattata
    17221 atgaacttaa aggcaataat ttaaatttta gtattgagaa aaataagcca aatattttaa
    17281 aaggtaaaac attattattt cttggtgcgt cttttactta tggtcacgct tcgtttggtg
    17341 aatcgtttgt cgaatatatt gaagtgcgga atgattgtac ttgtattaaa gaagccgtga
    17401 gcggcacaac acttgtggag catattgaag atagttatat tactaggcta aaaaaagtac
    17461 cgttaggcaa gaagtatgac gcattacttt gtcagctctc aagtaatgat gtgcggctta
    17521 aacaagaatt tggtgttatt aaagaaagtg attacgatac gaaaacaata tgtggcgcta
    17581 ttcaatatat tgcaaagtac gctcgtgatg tgcttaagtg tcctgttatt ttttacactt
    17641 gtccttattt tgataaggag cggtatcaaa aattagtaag tatacttaac gaaattgcta
    17701 cgaaaatgca gtttagtgta attgatatgt ataatgacaa aaacttcaat aatatttccg
    17761 cggagacata cgctttatat atggctgatc ctgttcatcc aactaaagcg ggatattatt
    17821 tttggcttac tccttacata gaaagccact tatttgcctt tttcactaaa ttaattagat
    17881 aatttgcatt aattaataat catgttataa tactaataca tcgcggagta gagcagtggt
    17941 agctcgtcgg gcccataatc cggaggtcgg aggttcaaat cctccctccg caaccaagtt
    18001 aacagcaaag gtttgataca atagaaacaa tgtatcaaac cttttttcgt acaaaacaca
    18061 tatttttgcc ttaacatatt aaaagtttta actctgagca agaaatacga gatgtttatt
    18121 atgttagctt gttcgcctat taaataaaat ttagtgcttt ttcttaaaaa agaaagtcaa
    18181 aaaccttttg aaataagccc aaaattgaaa atatatatga taaaattttt aaaacatatg
    18241 aaaaaaattc aaaggaacat tttcacggat tacggcttta caatcggttt cttgattttg
    18301 aagtagacga acatgaaatt tctttaattg taaatcgata aaaccttgta aaggcatggt
    18361 tgttcgactt catacttaaa tcatcgcttt acagaaaaag gaacaatcta atttgagaga
    18421 taatctaaag gaggaattat ggaatttaaa actgttaatt atgattcaaa aataataaaa
    18481 gaagggattt ctcataaagg aagacgcaaa agtgatgacc ctgtgaagat attttctcaa
    18541 caattaaaag atcaaaatgt tttatatttc ttctacaaag atgatgaatg cttgtatatt
    18601 ggtcagactg gcatttgtct ttgggatagg attattcgcc atgaggaccc agaaaaagat
    18661 tcaaaatggt ttgaagaagc aaataaaata tgtttaatta tccttgataa aaaagttgat
    18721 gttatatcta gaagaaactt agaatcaaca ttcatagtta attatttacg agctggccac
    18781 aaattatata ataaagagta atttcatttt taagactttt gaatataaaa gtattagcta
    18841 ttgtataaaa agagtttatt caagccaatt acaaaatgtt gccatttata aatgtttaaa
    18901 tgtaattttt ataggttctc aagattagat tatgtctttt aataaataat aatctcaagt
    18961 tgtaaaagca gaaaataaag agcataaatt taaaagtagt taacaaatga aaaaaactca
    19021 gtataaaata agccttaatt ttttgttata taatatggac aaagagaagt gatgttatat
    19081 gcgaaaatcg aaatataaac cattggcaga ttatttgtca gcatctaata aaaactctgt
    19141 aagtttaaca tttaatcaaa tagaaaaaat cctaggtttt aaattgccgc cgtcatctcg
    19201 aaaatatcgt gctaactggt caaataataa aatggaggca ttggcatggg gttggcttcc
    19261 agtaggatat gaatcctttg atgtgagaat gagtgacgaa attgcaaact ttaggaaagt
    19321 tggtaataaa ttaaaatcgg aaaaaagtaa aaagagtaat cgcaagcaga attcagacct
    19381 aaaacgacaa attgaaaatg caacagatct catatttcta gatgttccaa ataatttact
    19441 taaattagaa gttgatgaat attacatggt aaaactaaca aaaacaaatt cggagattgt
    19501 tgagcatgaa ttagctaatg acatttctta tcctcaaaaa gctaagaaaa tatgtgaaga
    19561 actaatcaag cgaaataatg atttttccag cgaaacattt aacattatta ttcatagaat
    19621 agcgattgaa aattcaacca ggagctccaa aaaaacgata gatttattaa ctgatttcgc
    19681 acttaattct gaaaatcaat ttttacaaag agtaaaaaag ggtgatttaa ctttagtaga
    19741 tgatatcact gattatctat ttaaaaataa taatagaaga gataagagtt tagcaagtaa
    19801 agtttgccgt tatctaaatg aatggttatt cgataaagat gacttcacta tcaatgattc
    19861 tgttgtacgc aaggttctac cgtactattt agcctactat aaaatcgaaa agcactattg
    19921 ggctaataag aatcttgata aattaaccta tgttgaattt tttgctattt ttgaaaaaat
    19981 aaaagaaaag ttacctgaac taacccgcca tgaattggat cacctgcttt ggtattctta
    20041 taaaaatgat aatatacgct caacaattgc tgcttcgctt gctaaacatt tataatttct
    20101 aatacaaagc aagtcagata caaatattat tcaatctaaa aagtaaagaa acactgcgta
    20161 aaaattatct tgatccagcg ttaaaataca atttagtgat tcgtgaatat ccgcttaaaa
    20221 gaacaagtcg taatcagcgt tataaattat ataaatgagg atttaaagat gaaatttgaa
    20281 agaacactag caatttgtct tgatatgaac ggctgcccaa atcgttgcct acattgctgg
    20341 ttaggtgact tacctaatca taagcttaat gataaaacgg cgacgaaaat tgtaaaactt
    20401 tttaaaccat attttgaaaa tattactttt tatagttggt taagggaacc agatttcacc
    20461 aaagattata aagctagatg gttaaaagat aataaacttt caactattaa accaaaacgc
    20521 tttgaactgg caagctactt tacaattatc agagatcctg aatatgttaa atggttaaaa
    20581 gcagtgggca caaaaaaagt gcaattaact ttttttggtt tagaagctat gactgataag
    20641 tatgtaggac gtaagggagc atataaagaa ttaatcgctg caactaaaat cttgcaggat
    20701 aacgatatcg atgtacgatg gcaattattt atcaatgaag aaaataagga tgaagtgtca
    20761 catttattag atgtagcggc agaaaataag gtcactgaca tatttgtgca tgaaggaagt
    20821 tgtgacggta ataatcgtaa actttatgat attcgtatta ataaagacaa tattcctctt
    20881 aaagttaaac cttattactt aaattatgaa aatcttctgt ctgagagtga atgtattgaa
    20941 cttttaaagg atgacgattc atattttatc ccgaaacaca aagatgaaat tgtcatttat
    21001 ataacgagtg atttaaatgt ttattttaat ttcactaatc ctagtgctgc ttggcaaatc
    21061 ggaaatattg aagatgacga cattagtgaa attgtgcgga aaattgtcgc aggtgatgtt
    21121 ccggctttaa aatttgccag aagtattttg cttagtgaac ttgtaaaatt atacggtgat
    21181 tcaaactccg ataaagtctt ttcacttgaa gattataaaa tgtatttact caataattat
    21241 gttgatgata aatataatag taaattacaa taaagagcaa aatagtgcat ttttgccagt
    21301 tttttagact taaaacatta gttagactat ttaattacaa ttaggtattt ttaggataaa
    21361 gtctaaattt aagatacaat tagaaaaata cttcatttaa aagcaaggag aaaggaaatt
    21421 gcaagacact tttaattaca acgattatca aaacgatcaa atagtaaaaa aatatgacgg
    21481 ccgtattaat cgtcatcatt taggtatatt attttggcgg ttaggtgttg cttttagtat
    21541 tatctttatt gttttactct tagcaagaac actcatttcg attactagca ttatcatttt
    21601 tatgatcggg cttgtcattc tttcttttat ctttttaatt acgcttggaa cgattttaat
    21661 tattgataaa aatcatggtc ggcactggaa aaacttaatt aatatactcg aaaacaggga
    21721 gactgcgctt ttagtaattg ataaaatact aacttttgtt ccgctaatat gtagtgtaat
    21781 aattgctttt aatatctttg cgcttatctt actctacact agtaagttac cagtgcgtcg
    21841 ctttcgttta gtaactgcta ttatcattat tattttaaat atcgtcgcat taatttatgc
    21901 cttagcggga ggaaacttta catgaagact ttaaagttaa gagtgcttaa cccacgtatg
    21961 cataatgtta tttatatgtt tgacggtaaa gcgctaaagc caaaaggtga taatatgggc
    22021 cactatgttt ttaatattga aaccccagcg gataaagtcg atatccttat tattcgccgc
    22081 tctccacttc gcagcagatt atggttagtg tggcaatttt tgttctttat tgtttcactc
    22141 cttggtattt tggatttaca gagtaaaaaa cttaataaag aagcaatata tagagccaca
    22201 ctttatttaa gtggtgaaga tgaagttgac ttaaaatttg acactgataa ttcgagtaac
    22261 gcttttgtgg aactaacaac aaccttacaa gttgaagaaa gagaaaataa aacacttagc
    22321 gacccactta ttgtgcggcg cgctaaagtg ctaaaaattc taaaaataat cacatatatt
    22381 gtgcttttaa ttactctaat tatcatctta attttaatta aaaaataatt taaatatcca
    22441 aaaataaaaa gccacatttt gcaatgtgac ttttcattta ggcaggactt attgagcagc
    22501 acttatggca ggaaacacta attaaattac cacttgttat gggagcgctc gaagaagcct
    22561 tgttggccag ttaattcaac cacagtaccg tcatctaatg taattgtacc gtagtagcga
    22621 ccataaactt ggtggcattt cataaagata actttgaagt cgttatcact atagcggtca
    22681 taaatagttt tcatgaccaa atctatctta ccatcatcgg acttaataat ccagtcagcc
    22741 atgtaatcga gcgttacatc gttttcaata tagaggcgac caagcttata agctttacca
    22801 tcaataatca tcatattttc actagcggcc tctaaattac caaaaccgta gcctaagttt
    22861 agattaatca ttcgtccatc tgcgaggtag tcatttactt gaccccagta ccatacttca
    22921 tggaatggcc acaccccacg accccagtca agtaagccaa agtcaacttt cgggtcaaaa
    22981 gtaaacgcta agtcatccat gctaaacgtt ccacttgcgc gtaaattacc aattttataa
    23041 ttgagataga aatgatacaa agaatcttca aatggtgtat taataaccat tgcatcgtga
    23101 ttctctaagc gttcaagttt aacatcaact ttaaatgtca cacctttttc attaaccatt
    23161 gagccgtata aatgacgaat tcgccctttg gtaacataat gaaggtcaac ttttttcttc
    23221 gtgtcggtgt aatgaatttc attatccact gatgggtcag ttggcatagt catgctctta
    23281 gcacccttga agagtttaac aacacttgcg gaataagttt cttcaccttc taaatcaatt
    23341 aaattaatcg cgatattttg tgcatatgca atatggccaa gtgtgagctg gacaacataa
    23401 cgcccatgtt gaatttgata gaagtcccac tctttaaggc ggagtggctt tttcactagt
    23461 tttttatcaa atacaaatga catatcagtc gcgtaaccga aattaatatg tcgtttttgt
    23521 ttatcaaata ctggggtagg agttgtgtaa tatagttcat caacacctaa ttcctcttta
    23581 gcggtactga ctgcttcact aatttttgta ttacgcatcc gtttataata tcctttgcgt
    23641 aagaagataa gcggaataag cgctagtacc ataataatag aagcgaaata gaaaagtgct
    23701 tcagtgggaa tatagcccat cacttttgtg ccgtcttcta aaacttcaac gactgtttca
    23761 atcccgaaat ggcgaatgat tggttgacta atacttgaac ctaacaccat cggtattaac
    23821 acaaagaaga taatgcgtaa tccttcaaat tgtcctttac taccaacagg gtagagcact
    23881 ttgctccaga caccgagcgt ttgcataatt aaaatataac ctgcaccaac aacaataaca
    23941 ccgagtagaa gcgggagatt tttggcgcca aataaattcg ctggatcatg ataaccacca
    24001 attaatccta aataaatgag gccaacaatc gtcattaaaa tcgaaattaa cgcaacgcgc
    24061 ggactcttgc cgcgattaat taagcgactg ccaggtatgg taaagagtat cgcaataatt
    24121 aagccgattc cttgaataat accagcatcc ccagcgttaa acccatagtt tgaaataaat
    24181 aaattaccaa tatggacaaa ataaacatta aaaccaataa agtaaatagc agcgacgaca
    24241 ttaacgagta caagttcttt ttgactaaag aaatatttaa agttaaaagc ttcggcgaat
    24301 tgacgccaaa aactaccgcg tttacttggc tctaattttg gtgaatcttt aagtaagaaa
    24361 agcgaaagga caccaattaa gctcacaaag ccgcccatca ttgagaagaa ggcaatatag
    24421 ccaaaagctt caattaaaat tccgcctagt actgtaccaa taatcgtgcc gatgacgggt
    24481 tgaaccgcaa gtgcggtacc gattgcccct ttattatcat cactaattaa gtcagcggtc
    24541 caggcattaa acccggcatc atttcccatt gatccgaaaa atgacataac tgtatcggca
    24601 aggacaataa cagtcgctag tgcccaaatg ttcatcgtaa attttgtaag tagaccagtt
    24661 aaaccaaaag caatcgtgaa gataccccaa atgatatagc caatgctgat gaatggtttc
    24721 cttttaccag cccggtcact aagtgtccca aagaagaatg tggcaaaagt tgtagcaatc
    24781 gccgaaacag cgaccattgc attgacaatt tgcggaaacg gaccaacttc agcgtaaaca
    24841 aatgtattaa accattggtt ttctaagttc caacataatt gtccagcaag accaacaagc
    24901 cacaatagta aaatgttgcg aaaacgcaaa cgacgacttt tcgttttatt attcataatt
    24961 ttttctccta ttaaatgtat tttatcttat ttttgctaga tgttaaacta ctttaataaa
    25021 taaataaaaa agctctctaa taaacggaaa aatacgaaat ataagtgaat tttaggcttc
    25081 tttttctgct tcgagaactt gctctttaac aaagaggctt ttaagcttaa tagcaattaa
    25141 acgaagtaag gcactaatag caaggacaga tgacaccgac attaatgggg caataaatgc
    25201 atctttttcg tcatcaatta aatataaccc atcatgcttt acaaaaaacc aattaaagtc
    25261 atgttcataa gaaagccaga agttacttcc gctatagagc gtattaccaa tataggccca
    25321 tactgctacc gctaagacgg caataacatc atatttaaag tttttccaac tcaaatctaa
    25381 atcatcaaat actagcgcaa aaaccccttg tgcgatcata agaccgtggt aaattactgt
    25441 ttgcacaata cggtaactat agccatcagc ttgtgccaca cctgcgggat aagtaatgta
    25501 cattaatccg ccgattagcc caagaagggt aaaggctgtt ttaaatactt ccattttttt
    25561 attgtgccgc actacaaaac acataacact cattaaggta cataagtgaa acggtaactt
    25621 accgatatca attgtgccgg tagcaaaagg catgaggaaa aagtcagcaa tataaagtgc
    25681 gaaggcaatt gtcacagtgg tagtaataag ttttgtcttt gcttcttgtg ttttattttt
    25741 gtataagaaa attactaaaa tgattccacc aataattaat aataaataaa taatatggca
    25801 taaactaaaa gccgagaaag taaagacacc cgtatcttta tccttaaata attcatgcat
    25861 tagattaaac atataatatc tttttaatta ttagtagtga ataattattc ctttctaaaa
    25921 atgacttaat tagttttatc agtagttaaa tcctcatctt gtttatttga tttagtcgat
    25981 attttagtta tccattgttc agctttgggc agggtgatgt gctcgtaaat tagcagtcca
    26041 ataaagcaca aaattagccc aatagaaccg atagtgaaga aattaataaa cggataatct
    26101 tcaaacggtg gttctaaaag aaacatcgca ttaacaggtg ggatatcaaa ttttgcataa
    26161 agtgtattaa taataatacc atcagcgaca aaaaagagaa gtccaactat aacactaatc
    26221 attgtttttt caacgcgtgg ttttacatac tcaaaaacaa ataagtatgt tgtaccaaaa
    26281 aacatcacca tatgaccaaa tacaccttta aaaagaaaat agttggtgaa tgttggatta
    26341 ctactattca aaaaattcat attaaagagt gctccaatta aagccgcaag agtgccaaca
    26401 acgaatacaa attctgctag tccttcaaag aagcgggact ttttatgacg cgtaaaagca
    26461 acgattacga gcaaccacat cataatgtga catggatata ccggtaaaat ataagaagcg
    26521 gatgcatatg ctacaccttg attttgaaag aattcaacat aaactgcact atagtgaata
    26581 agaataagta agattgaggt agtttttaaa ataagatcat gagttcgacg ttctttgatg
    26641 aatttcttgc ataaaaaaag caatactcca atcactatta gacatatgag attaaatatt
    26701 gataatttca tgaaagctgt cataaactat tcatctccta ttacaaccat ttcattatat
    26761 caaatgaaat ttaataaata ttactaattt gtaataaaag aaaaaaacgg acttgttcaa
    26821 gtccgttttc gcgcaataat ggtggcccaa gccggaattg aaccggcgac acgtggattt
    26881 tcagtccact gctctaccat ctgagctatc gggccttact ggcggaccag acgggactcg
    26941 aacccgcgat cttctgcgtg acaggcagac atgttaacca ctacactact ggtccagcct
    27001 atatactata ataaagttcc aacttttttg caagtatttc aatacgatgt tttgattcta
    27061 tgaattactt ttgcaaaaat actttagaat ttcgcttatt acatagtaat aaatgatagt
    27121 taacattatg tattgcctct taagatggcg ctaatcttgt ataataaaga tgatatatga
    27181 acaaaccttt agaaactaat taaggcaata ataaaaacaa taaatattta tttcaagatg
    27241 aaaaggagca atgaatatga ataataaagt accattaagt gaatacccac gaatgattat
    27301 ggttcgtgat tcttacttat cactaaacgg cgtatgggaa tacgctatta gggaaaatga
    27361 tgaaagagta ccaatcgagt ttgatggtga tattttagtg cctttttcac cagaaacacc
    27421 gctttctggt gttaaccgca cagtaagacc aaatgatgtt cttttttatc gtaaacgctt
    27481 ttcgttacca aagggtttta aaaaagaata tgtaacgctc cattttggcg cagttgatca
    27541 agttgtaact gtgtatttaa atggtactaa aattggcttt aatcaaagtg gtttccatcc
    27601 gttttctatt gaaattagta aagtcctaaa aatagacaat gaactagtgc tcgttgtccg
    27661 tgattatagt gacactaggt tttatgcccg tggtaaacaa aagattaaac gcggacaaat
    27721 atggtatacc ccacagagcg gtatttttat gccagtttgg cttgaaagta tgccgcgtga
    27781 ttatattgaa aaattaaaga ttactccgcg ctatgacgag tcacttgtgg agataactgt
    27841 tttcactaat tcaaaagata atgatgcagt aattattttt gatggaaaag aatatactgt
    27901 aaaacctaac aaagtatata agcttaaggt taaagacttt attagttggt cacctgataa
    27961 cccgcatctc tatcccttta aagtgaaata ctgtgaagat gaagttgatt cgtactttgc
    28021 gatgcgtaaa attagtgttg atacagatga agaagggatt aaacgcatat atttaaataa
    28081 taagatactt tttaatacgg gattactcga tcaaggctat tatcacgaag gttatttaac
    28141 accacccgat gaagcaacag ttattaatga aattaaagtc gcaaaagaga tgggctttaa
    28201 tgttttacgg aaacatatca aaattgaatg ggaacgcttt tattatcatt gtgatcgctt
    28261 agggatgcta gtgtggcaag attttgttaa tggtggcgaa ccgtataact tcgttactgt
    28321 acatgttcca gcagtgttaa atattcactt taaagataca aattataagc gttttgcacg
    28381 gcaaaatgcc cttggacgcg aattattcta cgctgaagct aaaacaacga ttgaacattt
    28441 atataactat ccgtccattg ttttatggac aatatttaat gaaggctggg gccaatttga
    28501 tagtcataaa ttactcccgt ttgtgcttga acttgataat actcgactaa tagatgttaa
    28561 ttcaggttgg catgatatgg ggcttggaca gttccgcagt catcatgttt attttcgtaa
    28621 atatcgcttt aaaaaagata agcttgatcg ctgcgttatc ctaagtgaat ttggcggtta
    28681 tgcgcatcgg atacctggac actattacgg ggtcgctaac tttggatatc gcaaatataa
    28741 cactaaagaa gaattagaaa aagcggtaat taacctgtat gaaaaagaaa ttattcctgc
    28801 agttaagcaa ggcctagcag cggcgatata tacccaatta agtgatgtcg aagacgaatt
    28861 aaatggtgta ttaacttatg accgaaaggt tgttaagatt gatccaaaag ttatgcgtga
    28921 acttaacgaa aaactcttaa aagcttaacc catttaaaga aaagacatta tctttgcgat
    28981 tcggttcctg ccaattatgc attgataatg tctttttaat attccgttac agaatatttg
    29041 taaagccatt ttattctaaa ttagaatatt aagcaattaa taaagtaaaa gcgttttagt
    29101 ttagttaaga taaaatcatg tattgcgaaa aattaattac tttacgtcgt aatcgtggtt
    29161 taacccagca gcaacttgcc gatgctttaa tgttgtcgca atcaatgatt tctaaaatag
    29221 aacgcggcat aactcggctt gatccaatgc tagcaattcg tttt
//
LOCUS       DAOSLP010000009        28500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_1850_length_28500_cov_7.859378, whole genome shotgun sequence.
ACCESSION   DAOSLP010000009 DAOSLP010000000
VERSION     DAOSLP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 28500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28500
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1850_length_28500_cov_7.859378"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK460_01665"
     CDS             complement(<1..546)
                     /locus_tag="PK460_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOE53839.1"
                     /translation="MKKLTSNDIRKMWLSFFKSKNHAVVEGASLIPVNDKTLLWINSG
                     VAALKQYFDGSKVSADKRLTNVQKSIRTNDIENVGKTNRHHTFFEMLGNFSIGDYFRT
                     EAITWGFEFLTSKDYLALPLEKLYFTYHPSDTETRDLWISLGVPKSHVIANEDNYWEI
                     GEGPCGPNTEIFFDLGEKIDAR"
     gene            complement(776..1924)
                     /gene="mnmA"
                     /locus_tag="PK460_01670"
     CDS             complement(776..1924)
                     /gene="mnmA"
                     /locus_tag="PK460_01670"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOE53840.1"
                     /translation="MKKDKANIKVMVGLSGGVDSAVALALLKKEGYDVEAAFMRNWDS
                     LLNNDVSGNPTLYDPTCPQEVDYNDAKAVAETLGVKLHRVDFVKEYWDNVFSYFLSEY
                     EVGRTPNPDIFCNKYIKFDAFLAWALEMGADYIATGHYAKLEEVNGELVMKKSADKNK
                     DQTYFLSQLSQAQLKRTLFPLGDIPKSKVREIAEKLDLSPAKKKDSTGICFIGERHFR
                     DFLKNYIPAQDGVIVDINTGKEIGTHTGVYYYTLGQRRGLGIGGLKDYPENKRWYVCK
                     KDVVNNILYVANDDEDEHLKSDKITLTDVNFINEELKSGTPLNVKLRYRQKDLPATYY
                     KNKDGAYLLFPKPYLAVTPGQAAVFYIDEVCLGGAIIGDLFYKGKKIN"
     gene            complement(1911..2354)
                     /gene="dtd"
                     /locus_tag="PK460_01675"
     CDS             complement(1911..2354)
                     /gene="dtd"
                     /locus_tag="PK460_01675"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOE53841.1"
                     /translation="MRVVIQIVKDAKLEIDNKVYSSIDYGMLLLVSFTLSDTKEVAKK
                     MAEKIALMRIFPDENGKTNLNINQVNGEVLSVSQFTLYGEFNSRRPSFTKVLSGEESS
                     QLYDYFNSELRKMLKVKTGVFGSDMNITFTNVGPVTYVVESDEKR"
     gene            complement(2354..3661)
                     /locus_tag="PK460_01680"
     CDS             complement(2354..3661)
                     /locus_tag="PK460_01680"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOE53842.1"
                     /translation="MRMYDIILKKRDGFALTEEEIKFFIKGVTDGTIPDYQTSSLLMA
                     IVWRGMNKEETAALTLAMMHSGDVIDLSAIKGVKVDKHSTGGVGDKTSLVLGPLVASF
                     GVKVAKMSGRGLGHTGGTLDKLESIPGTRIDIPNDDFIRQVNEVGCAIIGQTGHLVPA
                     DKKLYALRDVTATVEAIPLIASSIMSKKLASGSDTILLDVKFGSGAFMKTLKDAQELA
                     RAMVDIGKSLNRDTRAILTDMDQPLGLAVGNALEVKEAIATLKGEGPRDLSELCIEAA
                     CIMLEQAGVVKKAQDARKEVENKIKSGAALQKLVDMVKAQGGDESYITNPEKFAISQN
                     VFEVLATSEGYVEHIDSMAIGISSMKLGGGRETYEDEIDMSAGIVLNKKVGDKVKVGE
                     RLALVYTNKADEVSTPILVDIHHAFKLSDKHIDVPPIIHDYIK"
     gene            complement(3773..4570)
                     /locus_tag="PK460_01685"
     CDS             complement(3773..4570)
                     /locus_tag="PK460_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE53843.1"
                     /translation="MIKYIIWIVKVAIPLMWGYFTWMRPYARRPDKYPPELRMAKGQK
                     LARRVLRILKVDVEIRNMPDFKRDEIYYIVSNHTSVLDLVVLLAYLPIPVSFLSKVEN
                     KKIPFVRTLMRVYKGLYLERNNLRQEIKVMQMTRDSLANKEVSWLIFPEGTRSKDYHG
                     NLSDYKAGAFKAPLATNTTIVPVVMWGNQMVLPLRTRARRYKVIVEFLPLLKPEGTTL
                     ELANKIYEASNATASKLREEYGSVQKINKYGRKIMTLQLSENIKTSK"
     gene            complement(4580..4843)
                     /locus_tag="PK460_01690"
     CDS             complement(4580..4843)
                     /locus_tag="PK460_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53844.1"
                     /translation="MLTSVFIVGTLGKNENDYRYLLVEKVPGLDYEDDEERAKYDYFK
                     VKHWSNTPSAFNRLAEGRKVALKGRLEEIEGETYIIAELYREF"
     gene            complement(4863..7034)
                     /gene="pulA"
                     /locus_tag="PK460_01695"
     CDS             complement(4863..7034)
                     /gene="pulA"
                     /locus_tag="PK460_01695"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOE53845.1"
                     /translation="MAFGQFSAHLIAPRVIRVVLYSDKYQYEQTRVTIYDDNDKLVPF
                     SSKSKVVQYKVTAFELTLEQPFVLGQNYKVYLQNFGQVPLNVSEATTFPNFDTDYYYT
                     GPLGAEYTKEQTTFRLWAPLASRVILKYEVPGEKHFLYRKLKRGERGVYEYTLEGDHE
                     GIRYTYLVLNNGLEEEVTDPYALSSGPNGAFSYVINQNKYDDMNMHDDKLPPFTKPTE
                     AIIYETSVRDQTIDERTDIEYPATFYGMIEEGRKVKDTYPAGFDYLKDLGFTHLQLMP
                     VYDFKTVDETRKWQSYNWGYDPQQYFVLEGSYARDPLHPYNRISDFKLMVSRYHEVGI
                     RIIMDVVFNHVYDHETSVFEKIVPNYYFRKTSEGVISNGSGCGNDLATERPMVRRLVV
                     DACRYYAEKLGVDGFRFDLMGLIDIKTMEEVTKAVRQVKPDFLILGEGWEMMTPHPKE
                     KLAHHGNASKTPEIGFFNDAFREIVKGGSFADNLHERGYMLGATNYRLGFYYAYAGSV
                     LNTTFTPRYLNAGQSINYVECHDNGVLVDKMTVSNASECKETHLARLLSMNTIVMLSF
                     GVPFFHRGQEIGVSKYGDLNSYKSSDKVNQFPYTLALKRKEMVEYFKALTALRKDCKF
                     LHETDPKVIKNIIEFKDLPGGASQIVYNPDMDLSPYSHFNVYVNPTSEPIFISLEKPL
                     KTILSSGGYIAARSKEYFERTMVAPYSVIVLGLRKGDSGPK"
     gene            complement(7040..7360)
                     /gene="acpS"
                     /locus_tag="PK460_01700"
     CDS             complement(7040..7360)
                     /gene="acpS"
                     /locus_tag="PK460_01700"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOE53846.1"
                     /translation="MSIGIDIVYIPRFAGKEKLATRILSKNELEIYHMRPNKEEFLAG
                     RFAAKEAFLKAMKEGIGRVAFHKIDVVYKKDGRPEIHHDGNVYAVSISHDGDYAVAVV
                     QIKE"
     gene            complement(7363..9951)
                     /locus_tag="PK460_01705"
     CDS             complement(7363..9951)
                     /locus_tag="PK460_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOE53847.1"
                     /translation="MSEAKTNLLMQQQRLSRGLKGIIVALLALIGILKISIGGVINVP
                     VALLFGDLFVVFFALQVLFAFYLIFFPKAKSKKRVTLVFLSIFGLLIISSAFYAGNLR
                     NYMRDGTTFKDNLDVMVTLARDTFSFTPPSNAYFYNYGGILGEGLLFVLKDLPVWLII
                     IILVLANIGFATMLLWPLLVKIYAAIKEEIRDLKKRRRVLHEAGQLHKEEAPTEEDQV
                     PVTEIMNDNEVWGSNLLHRVGPSASEAEEKLGPPPALNVVKNENSGMHRAMPSEGFSA
                     FDEHFELPTLDTNEEEFEALDPTVFRTMEKVESEEDFQELERAVDEKGSFEAVSFTPH
                     HEEPTNTLNAEPKKDENVLREALEERYEPEPLPPLPEPVVKQENEQKPRISSNFANFR
                     LPKLDLLDDAIDNGEEAQNREFAAEYEAKINAFFTDFNVDARVFSWTIGSTFTRFEIQ
                     LGPTESVKAINPLLNDISIRLNGASVRFEEVLPGKATSGLEVANKFRTNVNFKDCMHK
                     IVANKRKKYLIPLGKDVAGNVILAPFTDLPHLLVAGATGSGKSVFIHTVLTSLIMMHS
                     PLELRLILIDPKRVELSAYNNIPHLITPIIKEPSEAKVALDRLIDEMENRYKLFEKTG
                     VGKLSDYNEIIEEMGGEKLPVIVAIVDEFADLFESEKEISNLVLRLVQKSRAAGIHLL
                     IATQRPSVQVITGNIKANIPSRVAFMTASVTDSMTILGCGGAENLLGYGDMLVDSITT
                     NYRGLLRVQAPYVQVREVLRVTNYLRNNYMTDFYPEFLDLKEKVNVYGGGGPVISDDL
                     YQAVLDFTLTQDTISISRLQQNFNLGWPRARQLYDQLMQNGIIEPPDEANSAKGAVVI
                     ANKRGN"
     gene            complement(10027..11715)
                     /locus_tag="PK460_01710"
     CDS             complement(10027..11715)
                     /locus_tag="PK460_01710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOE53848.1"
                     /translation="MNDEVKIFALGGLDEHGKNMYVIEINNDIFVLEAGLKYPDKSSP
                     GIDFIIPNHEYLITNKSRIKTYFISHGHDDQFGALPFIYKQAPAPIYTTKAAAMMLER
                     FTRRVGVKDVKYEIFTIEPSSQHNIAGHKFTFFQTTHSAMHSFGIALDTSQGTVVYSG
                     DFIIEYTNSARYKLDLNSIAKIAEKNVLCLLSESSGAEKAGYTSPTHRLAPNIVPIFR
                     ETKGRIFISLYDQSTYNIEELVQVVINAKRKIAFYDPNTEEYFRDFAKAGLSLIDERY
                     IISNDDLLRARDQDIVILMLGAGEKIYEKIIALSNRENPDKKIVLGKDDTFIMALPAP
                     ATLEVIATEALDSLYTTGCNVVNITRKLFTNMHAQEEDLRMLLSLLKPKYYIPVKGLY
                     RQQIANAQLALHSGLKFSHHNIFLLDNGVSVEFNDGVGRVKMSPLDLIPSGDVLVDGT
                     GIGDINRGVIEDRQRLSEDGIIIMAIAVNYDERTITAGPDVQMRGFVYVKDSEQLLKQ
                     IRQIFINTVSEALDARQFDIDGAVKETESRVEKYIFRETRRHPMILPLIRVINE"
     gene            complement(11798..12145)
                     /locus_tag="PK460_01715"
     CDS             complement(11798..12145)
                     /locus_tag="PK460_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOE53849.1"
                     /translation="MSSDKKVTCLVCPRGCIMTVSSDLKVSGNFCARGVPYALQEVKE
                     PKRNLTYVVQVEGREAPLPVRTDSMIAKHLIPDVVKLLRTVTIKPPVAFNAIIIANVF
                     DTGVNIISSAEIK"
     gene            complement(12138..13391)
                     /locus_tag="PK460_01720"
     CDS             complement(12138..13391)
                     /locus_tag="PK460_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE53850.1"
                     /translation="MAKKYDLIIVGGGSAGLGAAVAAYDAGIHNILLIEKEATFGGIL
                     NQCVHDGFGVHLFKKTLTGPEYAHYLVEMLKERPFDILLSSNVTDISDKQEVTVFVAG
                     QGVMLFKTKAIILTTGSYERSAGAINLPGDRGAGIMNAGQAQYFLNLKGYEFAKDVLI
                     VGSGDIGLIMARRLSLLGIKVHAVSEIMPHSSGLKRNIVQCLDDFNIPLYLSHKVTDV
                     RGKPRIEEVDIAELDDKFEIIPGTTKTFKVDTLLLAVGLIPYVALSDNLGIPTSKSRG
                     PVVNNNYQTQLPWFFVAGNALHIHDLADDAYFEGEEAGRAASLFINGKLKAPTNTLQV
                     VPGYGINYVIPNNLELPLNTEELVLKFRPKENYENKRIVVKIDDTVIFKKFFPFLFPS
                     ELVILKVKKELLVAGEKLIVEVSDE"
     gene            complement(13375..14805)
                     /locus_tag="PK460_01725"
     CDS             complement(13375..14805)
                     /locus_tag="PK460_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE53851.1"
                     /translation="MYDIIIIGAGVIGALIGREASKYELNILMLEKDNDVGNGTSAAN
                     SAIVHSGYDPKPGTLKAKFNVASVPLYKDLCSDLDVEFKRIGSLVVAQGEEELPRLDE
                     LKARAELNGVEVKMLNKAEVHALEPNLHDHIVGGLFAPTAGIVNPFELTAHAIENAIE
                     NGMTLKLNEEVVAIKKLEDGFKVTTKNGDYFSKVVINAAGVFADDIINMVSKADFEIT
                     PRKGSYFVLDKLTVPLVNTVLFPLPNIHSKGILVVPTTAGNTLIGPTSEQIMSKVDVG
                     ADYASLEFIRKNANRLLKDIPFHKTIRTFSGLRATPSTGDFIIEHDKYVKGLINVAGI
                     ESPGLASAPAISLYVVNELVKDLLPLKVKANFNPRVRPLIRLQRLSTKEIQAKVKENK
                     DFATLVCNCEKVTKAEIIDVLSRALPVNSVKAMKKRLRTGFGLCQGGFCTAKVIAIMS
                     EYYGIPVDEIAYNEKESYVVKHGKKV"
     gene            complement(14964..17126)
                     /gene="pnp"
                     /locus_tag="PK460_01730"
     CDS             complement(14964..17126)
                     /gene="pnp"
                     /locus_tag="PK460_01730"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOE53852.1"
                     /translation="MKKVYEMMFHGRKIVVETGEFAKQADGAVLVRYGDTAVLSTAVA
                     GNNPTNLDYFPLSVAYQEKMYAAGKIPGSFLRREGRETEHATLIGRLIDRPIRPLFAE
                     GFRNEVQVINTVLSADYDCTPEMTAMFGSSLALTISDIPFEGPIAGVQVGRVDGKFII
                     NPTTEENAKSDLQLTVAGTKRAINMVEAGANEVSEEDMLEALMLGHEAIKELCAFQEE
                     IQRDIGVAKREFPLFVPDESLVAELKEKYTGKLTEAISVFDKLERSAAIEAVKGEMVE
                     EYEAKEYESDDDKRRVISDVKALHEVMVSDEIRRLITHEKVRPDGRKVDEIRPLDSQV
                     DLLPRTHGSALFTRGQTQVLSVVTLGPKSDEQIIDNLTDEESRRFMHHYNFPPYSVGE
                     IKRLGGPARREIGHGALGERALLAVIPSDEEFPYTIRVVSEVLESNGSSSQASICASS
                     MALMAAGVPIKDMVTGIAMGLITSGPLDGNHPYTILTDIQGLEDHFGDMDFKVAGTSK
                     GITALQMDIKVRGISKEVLAESLAQAKKAREEIRANQLSAISAPREDVAEFAPKMGKM
                     TIDVDKIREVIGTGGKVINKIIEDNDGAKIDIDDDGLVVVYHMNRATINNVLATIKAI
                     VKEAKAGEIYEGKVTRVEPYGVFVNLFGNVDALVHVSDLDWAYVDDASKLYKIGDTLK
                     VKVTGVDEKGKVKASVKVFKAKPEGYTERKSGGRRNNK"
     gene            complement(17187..17456)
                     /gene="rpsO"
                     /locus_tag="PK460_01735"
     CDS             complement(17187..17456)
                     /gene="rpsO"
                     /locus_tag="PK460_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOE53853.1"
                     /translation="MALTKERIAEIVKEFGANEKDTGSTPVQVALLTEQIRELTEHMK
                     VHKHDYHSQRGLLKLVGQRRGLLDYYNRVNREGYLALIAKLGLRR"
     gene            complement(17589..18803)
                     /locus_tag="PK460_01740"
     CDS             complement(17589..18803)
                     /locus_tag="PK460_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOE53854.1"
                     /translation="MQVFNVKNNVYYIGVSDRGLRVFDIMMETKHGTTYNSYLIKEEG
                     GVTIIDGVKAGFTDKWLDNIRSLVDIKDIKYLVVQHTEPDHSGAIPALLALNPNIEVY
                     GSFMAMANLKEINNAPFISNVIKPKQELKLSERQSLFFYPVPNVHWPDTIITHDPVNA
                     IAFTCDFLGQHIAPQQLLYSKEPNKKIIEEEIKLYYDAIMAPFKSDVLKSVATLKSLD
                     VSMVLNSHGYILDTKEAVDWAINNYIKWSTVNKSEKLTVTVVFASAYGYTRSMSEAVI
                     SELEKASALVHVFEVPRDDLATIVARIEESHALLVASPTFVGDGIKPVYDVLNSLNPV
                     MCFGKYFSAFGSYGWSGEAVRNYMNRASELRFKKVDEGFKVRLKPSEKELEDTRLYAR
                     NFIMKIKAELNK"
     gene            complement(18804..19724)
                     /gene="ribF"
                     /locus_tag="PK460_01745"
     CDS             complement(18804..19724)
                     /gene="ribF"
                     /locus_tag="PK460_01745"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOE53855.1"
                     /translation="MIAKHLDLSRITSLAPSTALVVGTFDGVHIGHKYLVEEGFKAAD
                     NVAVLLVYTNKKLTKLHQKSGVLTSLEDRVEIFEKFGVKTVYLLNLGDELLKLEPCEF
                     INKVLVPLAPNSVIVGTDFRFGRGAKGTGEDLREFGKNVFTTRVLAPLYYENEKVSTS
                     LIKELLSKGKPKIAKSLLGRYYALTGLVTKGYGLGNKLGFPTANIALDPSYFVPAHGV
                     YFVRVKFRANYYFGMASLGYHPTVNKVNVPLLEVNIFNFNENIYHDALTVEFIDYLRP
                     EFKFESVDTLIKQLQKDRENCEFKIEKGEY"
     gene            complement(19721..20578)
                     /gene="truB"
                     /locus_tag="PK460_01750"
     CDS             complement(19721..20578)
                     /gene="truB"
                     /locus_tag="PK460_01750"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOE53856.1"
                     /translation="MNKGKNGILLVNKEPGYTSRKVGNMLSRKLKIKKVGHLGTLDPF
                     ASGLLIFAVGEGTKVLPYLEDEVKTYVATLYFGLLTTTLDPDGEVIKKEEVPPFSKAE
                     IEKVIKQFTGEVTQIPPTTSAIKIDGKRAYEYARKNIEVDMPSRVVEVYTIEVLEYNH
                     PLLVIEATVSKGTYIRTLAQDIALALGTNATTIKLVRTRHGDFSLEKAKLFEEVSEED
                     IIDPSLGLKKFPIYKLSDEEVKRVRNGMTIKLNASEEIVQIHNNSGVLQALYKKKGDN
                     YIALRGFNL"
     gene            20621..21817
                     /locus_tag="PK460_01755"
     CDS             20621..21817
                     /locus_tag="PK460_01755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE53857.1"
                     /translation="MKHFRKLLAKIKKADEKFNLIQSGDKIVIGISGGKDSIVLFEAL
                     RTYKRYSKKDFTLYPVILDLGFSGFDASSYTAFFKEREYDLIVNDSRDVSKILSVQKE
                     KQGLSKLPCSICSRMKKAAINEVANKLGANKVAFAHHIDDALETLFMNMIGGGRFATF
                     SPQMYLERAEITFIRPLILVDEKLIIRVQKDLKLPLLPYNCPNENKTRRDDIKSLLNA
                     IYKKYPEAKTNFELMLINDKEFDLWYKDYATLINDDCVIVKVTTPAHFNDALYIRYKV
                     FVEEQNIKVEDEYDTNEKDYTSLVLYVKGNKAATIRYKYDALARRAYLGRIAVLKEYR
                     GLKLGMTLITYVEQKLSVKYRPLTFHIGGQSHLLKYYESLGYKRVGESYMDAGILHYH
                     FEKKVI"
     gene            complement(21894..22235)
                     /gene="rbfA"
                     /locus_tag="PK460_01760"
     CDS             complement(21894..22235)
                     /gene="rbfA"
                     /locus_tag="PK460_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOE53858.1"
                     /translation="MPKSRNLSRNESLMLRHLSEIISYKVGDPSLGLATVSAVELTRD
                     YSYAKVYISFLGDGDVEAKMEILNNASGFIRHELSQKMNIRKTPELRFIYDDTFERVA
                     KLDELLKRTKK"
     gene            complement(22228..24054)
                     /gene="infB"
                     /locus_tag="PK460_01765"
     CDS             complement(22228..24054)
                     /gene="infB"
                     /locus_tag="PK460_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOE53859.1"
                     /translation="MSNEKKRISNVAHHSKKQSGPCVEGRKLIYQGPIKVSDLARLLK
                     LSEAQIIKNLFMKGQMVTINNVLSPETVAELCLDFNFDYDFKEETEDDCDFGLSHIVD
                     DPKDLKPRPPVVTIMGHVDHGKTTLIDTIRSSSIVSGEAGSITQAIGAYQKEFHGKKI
                     TFIDTPGHEAFTAMRSRGTKVTDIVILVVAADDGVMPQTIEAIDHARAAEVPIIVAIN
                     KMDKPGVDPEKVKNELLAQNVIAEEYGGDVIFQPISAKKGTGIDELLDAILVTAEMLE
                     LEANPKRYAYGTVLEARLERGEGPKAMLLIQNGTLTNRDFVVAGAAYGKVRRMTNEHN
                     KALKSAGPATPVAIIGLDEVPEAGDNFIAFETEKEAKELATKRKQIRIEESRGSSEGM
                     KLSDLHKQIAEGETVVVNVIIKADNSGSAEAVRGSLEKINVEGVRLNIIRASAGQITE
                     SDVLLASASNAVIFGFNIRPNNQVRDLAERLKIEIHLHRVIYALLEQTEQALKGLLKP
                     EFEEKVTGQAEVRAIWHASKVGTIAGCYTLSGVIRRNQTVRLLRDGVIIYEGEVATLK
                     RVKDDIKETRAGYECGMTLLNYNDIKEGDIIEGYAMVEVKDA"
     gene            complement(24056..24358)
                     /locus_tag="PK460_01770"
     CDS             complement(24056..24358)
                     /locus_tag="PK460_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53860.1"
                     /translation="MTNELNNFLHLLKRSGHYRFGADYLRESNARKIALVLTSDDLSE
                     YTKTKVNNFTLAHNIAQFDVPANIMSMLYPGKTIKVLVVTNHQSAQKIANLMKEGN"
     gene            complement(24351..24608)
                     /locus_tag="PK460_01775"
     CDS             complement(24351..24608)
                     /locus_tag="PK460_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOE53861.1"
                     /translation="MKKIIVRRCIVTHESHEKRDLIRVVRTPDGRVVIDNTGKVNGRG
                     AYLVRNKEVILLAQKKNALSQALNIEVDQVIYEKLLEEVND"
     gene            complement(24619..26286)
                     /gene="nusA"
                     /locus_tag="PK460_01780"
     CDS             complement(24619..26286)
                     /gene="nusA"
                     /locus_tag="PK460_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOE53862.1"
                     /translation="MSKKLTKAQLRKKQERFEAMREMFREVAEKHNLKEEVVLNVFMR
                     AVQNVVARQKGGDDVHVEVVINEEMFDFEARVLKRVVAHDDDIEDDALEVCLEVARTF
                     DADAEVDALVVVERSFLGDFNSKLSNNVAHNFRHQLIELEKEQLFTLFKDKIGELVKG
                     TVEKNERITYINLLGITSVFFDERGRHAIPGEVFKPGQELNFYIVDVVSGAKGANIII
                     SRSHPNFVKRLMEREIHEIYDGTIVIHDIVREEGERVKVAVESLDPNVDPIGACIGPN
                     ASRIQKITAELGPDGRKEKIDIIPWVKNEALFIIEAFRPAVPLGLKIDEENKTAQVVF
                     SEEEKGKAFGHKGANIRLASKLTGYDLTILDLNEASEQGVEFLTKADIEYEERLAEQE
                     RTRERILRELARAEEVAQRAASQKSLLEEEVNSLNIDDLEYEDAFKDEEKVVETPKVV
                     TPEIKEEKKEPVVQKVKTTISLADLEAELAKEKEEIEQAQQKREERRSRTKQEVKQPE
                     VVEPTLPTGPKMDIYTEEELAELDAELEEVYEDDDLDFEDFDEYYDN"
     gene            complement(26301..26756)
                     /locus_tag="PK460_01785"
     CDS             complement(26301..26756)
                     /locus_tag="PK460_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53863.1"
                     /translation="MDLNLLQPKVEELLKERGYRLVSMRFLNEEGTRFLRVIVDKYQH
                     IISLDEIVAISEAIDAILDDDEESAFTLDITTTGAEKEIALQEVNDYLGLFIKLTMKD
                     GVKGPNTVKGILEAVSENEVTISHNDKGRIKKETYAQSDIKTINRAVKC"
     gene            complement(27006..27737)
                     /locus_tag="PK460_01790"
     CDS             complement(27006..27737)
                     /locus_tag="PK460_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53864.1"
                     /translation="MGNENLMLQHESKHYIFNYYAGSKAAEDILLIAEHQQACYTHIC
                     KVLGVEAKFKIKYFLCNSPEEVGKIYGDNEPCNAFTSSPDSIYAVYNDDIQCIGFHED
                     AHIISYTINRPESAAIREGLAMYFDRVWWGISNEDWTLYYLKTNKYISICNLLDNKYF
                     FSEDCSLTYPIMGAFTNYLIVHYGIKKYLVFYRYKDNIFDGFEKVYKLSVNELEMIFK
                     KEIAKLELTNDTESKIKELLMKNKT"
BASE COUNT         8483 a         6040 c         4598 g         9379 t
ORIGIN      
        1 acgcgcatca attttttccc ctaaatcaaa gaaaatttcg gtattgggac cacacggtcc
       61 ttccccaatt tcccaataat tatcttcatt agcgataaca tgactcttcg gcacacctaa
      121 actaatccat aagtcacgcg tttctgtgtc acttgggtga taagtaaaat aaagtttctc
      181 aagcggtaaa gctaagtaat ctttactcgt taaaaattca aagccccagg taattgcttc
      241 cgtacggaaa tagtcaccaa tactaaaatt acctaacatc tcaaaaaagg tgtggtgacg
      301 atttgtttta ccgacatttt caatatcatt cgtgcgaata cttttttgga catttgttaa
      361 acgtttatcc gcacttacct tactaccatc aaaatattgt ttaagcgctg cgacgcccga
      421 attaatccaa agtagtgtct tatcattaac cggaatcaaa ctagcgcctt ccactacggc
      481 atgatttttg cttttaaaaa aggacagcca cattttgcga atgtcattac ttgttaattt
      541 tttcataaaa aacctcaact aacataggaa cgactctcgc cgcggtacca tcctaattca
      601 tcgttgtgat gcgctttatt ttaattttgt aacggtaatt aaccggcgat gattagtcgc
      661 tcgaaggaag tggcacgttt tacttttgag tcctttcacc aactaactct cgcttttaag
      721 tttgtaaaac ttagtttcct tattgatttc gataatatta tagcatagtt aacgattagt
      781 taatcttttt gcctttataa aataagtcac caataatagc accgccaaga cacacttcgt
      841 caatatagaa aactgctgct tgacctgggg tcacggctag ataaggtttg gggaataata
      901 agtaggcacc gtctttattt ttataataag tggcaggcaa gtctttttgc cgataacgga
      961 gcttaacatt taagggagtt ccactcttta attcttcatt aataaaatta acatcagtca
     1021 aagtaatctt atcacttttt aaatgttcat cttcatcatc attagcgaca tataaaatat
     1081 tatttaccac atctttttta catacatacc agcgtttatt ttctgggtaa tctttaagac
     1141 caccaatccc taaaccacgt ctttgaccta aggtgtaata atatactcct gtgtgcgtcc
     1201 ctatctcttt accggtatta atatcgacaa tcacaccatc ttgcgctggt atgtaatttt
     1261 ttaaaaaatc acggaaatgt ctttcaccga taaaacagat cccggtacta tctttctttt
     1321 tagcaggact taaatcaagc ttttcagcaa tttcccgtac tttactttta gggatatccc
     1381 caagtgggaa taaagtacgc ttaagttgcg cttgtgagag ctgacttaag aaataagttt
     1441 gatctttatt tttatctgcg ctttttttca taacaagttc gccgtttact tcttctagct
     1501 tagcgtagtg cccggttgca atataatctg cgcccatctc taatgcccac gctaaaaacg
     1561 cgtcaaattt gatgtattta ttgcaaaaga tgtctggatt tggggtgcga ccaacttcat
     1621 attcacttaa aaaataacta aaaacattgt cccaatattc tttaacaaag tcgacacgat
     1681 gaagtttaac gccaagcgtt tctgctactg cttttgcgtc attataatca acttcttgcg
     1741 gacaagttgg gtcgtaaagc gtggggttgc cgctaacatc attatttaaa agtgagtccc
     1801 agttccgcat aaaagccgct tcaacatcgt agccttcttt ttttaagagc gctaaagcaa
     1861 cggcactatc aacgccgcca ctaaggccaa ccattacctt aatattagct ttatcttttt
     1921 tcatcgcttt ccaccacata agtcactggg ccaacattag tgaatgtgat attcatatca
     1981 ctgccaaata ccccagtttt aacttttaac atttttctaa gttcgctatt aaaatagtcg
     2041 tagagctgac tactttcttc cccgcttaag actttggtaa aagaaggtct tctgctatta
     2101 aattcaccat aaagagtaaa ctgactaaca cttaaaactt cgccattaac ttgattaata
     2161 tttaaattag tttttccgtt ttcatcggga aatatacgca taagtgctat cttttccgcc
     2221 atttttttcg ctacttcttt agtgtcacta agcgtaaaag atacaagtaa aagcattccg
     2281 taatcaatac ttgaatacac tttattatct atttctaatt tagcatcttt aacaatttga
     2341 ataacaacac gcattactta atataatcat gaataattgg tgggacatca atatgcttat
     2401 cgctaagttt aaaggcatga tgaatatcaa ctaagattgg agtagaaact tcatcagctt
     2461 tattcgtata tacgagcgct aggcgttcac caactttcac tttatctcct acttttttat
     2521 ttaaaacaat tccggcggac atatcaatct catcttcata agtttcgcgg ccaccaccta
     2581 atttcatact agaaatacca attgccatac tatcaatatg ttcaacataa ccttcactag
     2641 tcgctaatac ttcaaacaca ttttgactaa tcgcaaactt ttccgggtta gtaatataac
     2701 tctcgtcacc accttgtgct ttcaccatat cgacaagttt ttggagcgca gcacctgact
     2761 taattttatt ttcaacttct ttgcgagcgt cttgtgcttt ttttacgaca cctgcttgtt
     2821 caagcataat acacgctgct tcgatacata attcacttaa gtcacgcggt ccttcgcctt
     2881 taagcgtggc gattgcttct ttgacttcaa gtgcattacc gactgcaagg cctagtggtt
     2941 gatccatatc cgttaaaata gcccgtgtat cacggtttaa actcttacca atatcaacca
     3001 tcgctcgcgc taattcttgg gcgtctttta gtgtcttcat aaacgcaccg ctaccaaatt
     3061 taacatcaag taaaatcgtg tcactgccgg aagcaagttt ctttgacata atggaactag
     3121 caataagcgg aatagcttca acagttgctg tcacatcacg aagcgcatat aacttcttat
     3181 cagcgggtac taagtgcccc gtttgcccaa taatcgcaca acctacttca ttaacttgcc
     3241 gaataaagtc atcatttgga atatcaatac gcgtacctgg aatactttca agtttatcaa
     3301 gtgtcccgcc ggtatgccct aagccgcgcc cactcatttt ggcgacttta accccaaaac
     3361 tagcgacaag cggaccaagc actaagcttg ttttatcacc aacaccaccc gtgctatgtt
     3421 tatcaacttt aacaccttta atggcactta aatcaataac atcaccacta tgcatcatcg
     3481 ctagtgttaa ggcggcggtt tcttctttat tcattccgcg ccacacaatc gccattagta
     3541 aactactcgt ttgataatca ggaatagtcc cgtcagttac tcctttaata aagaatttaa
     3601 tttcttcttc agtaagtgcg aaaccatcgc gttttttcaa aataatgtca tacatccgca
     3661 tagttaatat ctcctttatt aaaactattt taccatacta aaaagcgctt actctactca
     3721 gatagagtaa actatacttt ttatcgtcat ttttttcatt aaattaagca aattatttag
     3781 atgttttaat attttcacta agttgcagcg tcataatttt acgcccgtat ttattaattt
     3841 tttggacact tccgtattct tcacgaagtt tactagcggt cgcattactt gcttcatata
     3901 tcttattagc gagttcaagc gttgtgcctt caggttttaa aagtggtaaa aattcgacaa
     3961 ttactttata acgccgcgct cttgttctta gtggtaagac catttggtta ccccacatca
     4021 caacggggac aatcgttgta tttgtcgcta agggagcttt aaaagcaccg gctttataat
     4081 ctgaaagatt accgtgataa tctttactgc gcgttccttc ggggaaaata agccaactta
     4141 cttctttatt agcaagagag tcacgagtca tttgcattac tttaatttct tggcgtaagt
     4201 tattgcgttc aagatataaa cctttgtaca cccgcatcag tgtccgcaca aacggaattt
     4261 tcttattttc gacttttgat aaaaaggaca cggggattgg aagatacgca agtaagacga
     4321 caagatctaa cacactcgtg tgattgctaa cgatgtaata aatttcgtca cgcttaaagt
     4381 ctggcatatt acgaatctcc acatcaactt ttaaaatgcg tagcacacgc cgtgcaagtt
     4441 tttgtccttt tgccatccgc aattcgggag ggtacttatc aggacgccgt gcgtaggggc
     4501 gcatccatgt aaaataaccc cacattaagg ggattgctac cttcactatc caaataatat
     4561 acttaatcat aatctctttt taaaactcac gatataattc ggcaataata tacgtttctc
     4621 cttcaatttc ttcgagtcga ccttttagtg caactttacg tccttcggca agcctattaa
     4681 aggctgaagg tgtattcgac caatgtttga ctttaaagta gtcatacttt gctctttctt
     4741 catcatcctc ataatctaac cctggcactt tctcgacaag taaatagcga taatcatttt
     4801 cgtttttacc tagggtacca acgataaata cggatgttaa cataatcctt cctcctatta
     4861 gtttattttg ggccagaatc tcctttccgt agtcctagca caattacact atagggggca
     4921 accatcgttc tttcgaaata ttctttcgag cgagcggcga tatatccgcc actacttaaa
     4981 attgttttaa gtggtttttc taaactaata aagattggtt cactagtggg attaacatac
     5041 acattaaagt gactatacgg gcttaaatcc atatcgggat tgtacacgat ttgacttgcg
     5101 ccacctggta aatctttaaa ttcaattata ttcttaatga ctttaggatc agtttcatgt
     5161 aaaaatttac aatctttgcg tagcgccgtt aaagctttaa aatattcaac catttcttta
     5221 cgtttcaagg ctaaagtata cgggaattgg ttaactttat cgctgctctt atatgaattt
     5281 aagtcaccat attttgacac accaatttct tgtccgcgat ggaagaaagg gacaccaaaa
     5341 gagagcatga caatcgtatt catactaagg agtctcgcta aatgagtttc cttacattca
     5401 ctagcgttac taaccgtcat tttatcaact aaaacaccgt tatcatgaca ttcaacatag
     5461 ttgatacttt gtccagcgtt aagatagcgg ggcgtaaatg ttgtattaag cacactacca
     5521 gcgtaagcat aataaaagcc taagcgataa tttgtggcgc ctaacatata accgcgttca
     5581 tgaaggttat cggcaaaact accgccttta acaatttcgc ggaacgcatc attaaagaag
     5641 ccaatctctg gtgtcttact agcgttaccg tgatgcgcta atttttcttt aggatgcggg
     5701 gtcatcatct cccagccttc acctaaaatt aagaaatcag gttttacttg gcgcacggct
     5761 tttgtgactt cttccattgt tttaatatca attaaaccca ttaagtcaaa acggaacccg
     5821 tcaaccccta atttttccgc gtaatagcga caggcatcaa cgacaaggcg ccgtaccatc
     5881 gggcgttccg ttgctaaatc attaccgcaa ccactgccat tactaatcac accttcactt
     5941 gttttacgga agtagtaatt tggtacaatc ttttcaaaaa cactcgtttc atggtcataa
     6001 acatgattaa aaacaacgtc cataataata cggattccca cttcgtgata acgactgacc
     6061 attagtttaa aatcactaat acggttatag ggatgaagcg gatcacgcgc ataacttcct
     6121 tctaacacaa aatattgctg cgggtcatag ccccagttat agctttgcca tttacgcgtt
     6181 tcatcaacgg ttttaaagtc ataaacaggc attaattgta agtgtgtaaa tcctaaatct
     6241 tttaagtaat caaatccagc agggtaagta tcttttacct tgcgtccttc ttcaatcatc
     6301 ccgtaaaatg tcgccggata ctcaatatcg gtgcgttcat caattgtttg gtcacgcaca
     6361 cttgtttcat aaattattgc ttcggtaggt ttagtaaatg gcggtaattt atcgtcatgc
     6421 atattcatat catcatattt attctgatta atgacataac taaaagcacc atttggtcca
     6481 ctgctaaggg cgtaagggtc agttacttcc tcttctaaac cgttatttag cactaagtaa
     6541 gtataacgaa ttccttcatg atcaccttca agcgtgtatt catacactcc gcgttcaccg
     6601 cgtttaagtt tgcgatataa gaaatgtttt tcacctggta cttcatattt taaaatcacg
     6661 cgactcgcta gcggtgccca gagtctaaat gttgtttgtt ctttagtgta ctccgcacca
     6721 agcgggccag tgtaataata atcagtgtca aaattaggaa aagttgttgc ctcacttaca
     6781 ttaagcggca cttgcccaaa attttgtaaa taaactttat aattttgtcc taacacaaaa
     6841 ggttgttcta gtgttaattc aaaagcagta actttatatt gtacgacttt actcttcgaa
     6901 ctaaacggca ctaatttatc gttatcatca taaattgtca cacgtgtttg ttcatattga
     6961 tatttatcac tatagagcac cacgcggatt acccgtggcg caattaaatg tgcgctaaat
     7021 tgtccaaatg ccataaatac tactccttaa tctgtactac tgcgaccgca taatcaccgt
     7081 cgtgactaat tgacacagca taaacattac cgtcatgatg aatttcaggg cgtccgtctt
     7141 ttttatacac aacatcaatt ttatgaaaag caacgcggcc tatcccttct ttcattgctt
     7201 ttaaaaacgc ctctttagcg gcaaaacgcc ctgcaagaaa ttcttcttta tttggtcgca
     7261 tatgatatat ttccaattca tttttcgata agatgcgtgt cgcaagtttt tctttacccg
     7321 caaagcgcgg aatataaaca atatcaatcc caatcgacat aattaattac ctctcttatt
     7381 ggcaataaca accgcccctt tagcgctatt agcttcatca gggggttcaa taatgccatt
     7441 ttgcattaat tgatcataaa gttgacgggc acgcggccat cctaaattaa agttttgttg
     7501 taatctgctg atagaaatcg tatcttgcgt taaagtaaag tcaagcactg cttgatagag
     7561 gtcatcacta ataacgggac cgccaccacc atagacatta actttttctt ttaaatctaa
     7621 aaattccgga taaaaatcgg tcatatagtt attgcgtaaa taattagtga cgcggagtac
     7681 ttctcgcacc tgtacataag gcgcttgtac acgtaatagt ccgcgataat tagtcgtaat
     7741 actatcaact aacatatccc cgtagccaag taagttttcc gctccaccgc aaccaagaat
     7801 cgtcatacta tcagtcacgc ttgccgtcat aaaggcaaca cggctaggaa tattagcctt
     7861 aatattacca gtaataactt ggacacttgg tctttgtgtt gcaattaata agtgaattcc
     7921 agccgcgcgg cttttttgca caagccgtaa tacgaggttt gatatttctt tttcactttc
     7981 aaaaagatcg gcaaattcat cgactatcgc gacgataacc ggtaattttt cgccacccat
     8041 ctcttcgata atttcgttat aatcgctaag tttacctacc cccgtttttt caaaaagttt
     8101 atagcgattt tccatctcat caatgagccg gtcaagcgct acttttgctt cacttggctc
     8161 tttaataatc ggtgtgatta aatgcgggat attgttataa gcagaaagtt caacgcgttt
     8221 tgggtcaatt aatattaatc gtaattcaag tggtgaatgc atcataataa gggaagttaa
     8281 tacagtatga ataaacacac ttttaccgct gcctgttgcc ccggcaacaa gaaggtgcgg
     8341 gagatcggta aacggcgcta aaataacatt accagcgaca tctttaccaa gcggaattaa
     8401 atatttctta cgcttattcg cgacaatttt atgcatacaa tctttaaaat taacattcgt
     8461 tctaaatttg ttcgctacct ctaagccact tgttgcttta cctggtaaaa cttcttcaaa
     8521 acgcacagaa gcaccattta gtcgtattga aatatcattt aaaagcggat taattgcttt
     8581 aacactttct gtcggaccaa gctgaatttc aaagcgggta aatgttgagc caatcgtcca
     8641 gctaaacacc cgagcatcaa cattaaagtc cgtaaagaac gcattaatct tagcttcata
     8701 ttccgcagca aattcgcgat tttgtgcttc ttcaccatta tcaatagcgt catcaagcaa
     8761 atcaagttta ggtaatctaa agtttgcgaa attcgaagaa atacgcggct tttgttcatt
     8821 ctcttgcttc acgactggtt caggaagtgg tgggagcggt tcaggttcat agcgctcttc
     8881 tagagcttcg cgtaaaacat tttcgtcctt tttaggttca gcgttaagtg tattagtagg
     8941 ttcttcatga tgcggggtaa aactaacagc ttcaaaactc cctttttcat cgacagcccg
     9001 ttcaagttct tggaaatctt cttcactttc gactttttcc atcgtgcgaa aaacggtcgg
     9061 atcaagtgct tcaaattctt cttcattagt gtcaagtgtt ggtaattcaa aatgttcatc
     9121 aaaagcggaa aaaccttcgc taggcatcgc gcggtgcatc ccgctatttt cattttttac
     9181 aacattaagt gctggcggtg gccctaattt ttcttcagct tcactagcgc taggaccaac
     9241 acgatgcaat aaattactgc cccatacttc gttatcgttc attatttccg taacggggac
     9301 ttggtcttct tctgtgggcg cttcttcttt atgtaattga ccagcttcgt ggagcactcg
     9361 tcttcttttc tttaagtcac gaatttcttc tttaatggct gcatatattt tcacaagcag
     9421 tggccagagt aacattgtcg caaaaccgat atttgctaac actaaaataa taataatgag
     9481 ccacactggt aaatctttaa gtacaaataa gaggccttca cctaaaatac cgccataatt
     9541 ataaaagtaa gcatttgacg gtggcgtaaa agaaaatgta tcacgggcaa gcgttaccat
     9601 tacatctaaa ttatctttaa aggttgtccc gtcacgcata taattgcgta aattaccagc
     9661 gtaaaaggcg ctactaataa ttaataagcc aaaaatacta agaaacacta gggtaactct
     9721 ctttttactt ttagcttttg ggaaaaagat taaataaaaa gcaaataata cttgtaaagc
     9781 aaaaaagacg acaaataagt cgccaaataa aagtgcaacc gggacattaa tgacgccacc
     9841 aatcgaaatt tttaaaatcc caataagtgc caaaagcgcg acaataatgc cttttaagcc
     9901 acggctaagt cgctgttgtt gcataagtaa atttgttttt gcttcactca tacttaatat
     9961 tatacataat attacttgaa tactcgaaaa agagagcaaa aattaataaa tttttgtgat
    10021 taattgttat tcgttaatta cccgaatcag cggtaagatc atcgggtgcc gtcttgtttc
    10081 acggaaaata tacttttcaa cacgtgattc agtttctttt accgcgccat caatatcaaa
    10141 ttggcgcgcg tctagtgctt cacttacagt attaataaag atttgccgaa tttgttttaa
    10201 tagttgttca ctatctttaa catacacaaa gccacgcatc tgcacatcag ggcctgcggt
    10261 aattgtgcgt tcatcataat taacggcaat tgccataata ataattccgt cttcacttaa
    10321 gcgttggcgg tcttcaatga ccccgcgatt aatatcaccg attcctgtcc cgtccactaa
    10381 cacatcacca ctaggaataa ggtcaagtgg actcatcttc acgcgcccga cgccatcatt
    10441 aaattcaaca ctgactccgt tatctagtaa gaatatatta tgatgactaa actttaagcc
    10501 actatgcagc gcgagttgag cattagcgat ttgttgccgg tataaacctt taacaggaat
    10561 ataatatttt ggttttaaaa gtgagagtaa catccgtaag tcttcttctt gcgcatgcat
    10621 attcgtaaat aatttgcgtg taatattgac gacgttacac ccagttgtgt ataaggagtc
    10681 aagtgcttcg gtggcaataa cttcaagtgt cgctggagca ggcaaagcca taataaaggt
    10741 gtcatctttg ccaagtacaa tctttttatc aggattttcc ctattggaaa gggcaataat
    10801 cttttcatag attttttcac cagcgccaag cattaaaata actatatctt ggtctctagc
    10861 gcgtaataag tcatcattac taataatata acgctcatca attaagctta aaccagcttt
    10921 tgcaaaatct ctaaagtatt cttcagtatt tggatcgtaa aaagcaattt tccgtttagc
    10981 gttaatcaca acttgcacta attcttcgat gttataagtt gattgatcat aaagcgaaat
    11041 aaagatacgc ccctttgttt cacgaaaaat tggtacaatg tttggtgcta ggcgatgtgt
    11101 aggcgaagta tatcccgcct tttcggcacc actagattca ctaagtaaac ataacacatt
    11161 tttctcggca attttggcaa tgctattaag atcaagttta tagcgtgcac tgttcgtata
    11221 ctcaataata aagtcaccag aatacacaac cgtgccttga ctagtgtcta gcgcaatccc
    11281 aaagctatgc atcgccgagt gtgttgtttg gaaaaaagta aacttatgac cagcaatatt
    11341 atgctgactt gatggttcaa tcgtaaatat ttcatactta acatctttaa caccaacacg
    11401 acgagtgaag cgttcaagca tcatcgccgc ggcttttgtc gtgtaaatcg gtgcgggtgc
    11461 ttgtttatag ataaacggta atgcaccaaa ttgatcatcg tggccgtgac taataaaata
    11521 agttttaatc cgacttttat tcgtaattaa gtattcgtga ttcggaataa taaaatcaat
    11581 accaggtgaa gatttatctg gatactttaa cccagcttct aacacaaaaa tatcgttatt
    11641 aatttcaata acgtacatat ttttgccgtg ttcatctagc ccccctaatg cgaaaatttt
    11701 tacttcatcg ttcataatta tacctcttgt taataatatg tgatatctct actttgttag
    11761 tataacaaaa gtttagctaa actaccaagt aattacttta tttaatttcg gcactactaa
    11821 tgatattaac accagtatca aagacattgg caataataat agcgttaaac gcaactggtg
    11881 gcttaatcgt aacagtgcgt aataacttga caacatcggg aattaagtgc tttgcaatca
    11941 tcgagtcagt acggacgggt aaaggtgctt cacgcccttc aacctgcaca acataagtta
    12001 aattgcgttt aggttctttt acttcttgga gcgcataggg gacgccgcgc gcacaaaagt
    12061 taccgcttac ttttaagtcg ctgctcacag tcataataca accgcgcgga caaacaagac
    12121 aagttacttt tttgtcacta ctcatcactt acctccacga tgagtttttc accagcgact
    12181 aatagttctt tttttacttt taaaatcact aattcgctcg gaaataaaaa cgggaagaat
    12241 ttcttaaata tcaccgtatc atcaatcttt acaacaatcc ttttattttc ataattttct
    12301 tttggtctaa atttaagcac taattcttca gtgtttaagg ggagttccaa attattagga
    12361 ataacataat tgatgccgta gcctggcact acttgtagcg tgttagtggg cgcttttaac
    12421 tttccgttaa taaataaact agccgctctt ccagcttctt caccttcaaa ataagcatca
    12481 tccgctaagt catggatatg gagtgcgtta cccgctacaa aaaaccatgg taactgtgtt
    12541 tgataattgt tatttactac tggcccccgt gatttactcg tgggaatccc aagattatca
    12601 ctaagcgcaa cgtaaggaat aagcccgacc gctagaagca gtgtgtcaac tttaaatgtt
    12661 ttagttgtcc ctggaatgat ttcaaactta tcatcaagct cagcgatatc aacttcctct
    12721 atccgtggct taccgcgtac atcagtgact ttatgtgata aatatagcgg gatattaaag
    12781 tcatctaaac attggacaat attgcgtttt aagccgcttg aatggggcat aatttcacta
    12841 acagcgtgca ctttaatccc gagtaaacta agccgacgag ccataattaa gccaatatca
    12901 ccagagccaa caattaagac gtctttggca aattcataac ctttgagatt taggaagtat
    12961 tgagcttgac cagcgttcat gattccagca ccgcggtcgc ctggtaaatt aattgctccc
    13021 gcacttcgtt cgtatgaccc tgttgttaaa ataatcgctt ttgttttaaa aagcataaca
    13081 ccttgaccag cgacaaatac tgtcacttct tgcttgtcac taatatcagt gacattacta
    13141 ctaagtaaaa tatcaaaagg tcgctctttt aacatttcga caagataatg ggcatattct
    13201 ggtcctgtta atgttttctt aaataaatgt acaccgaacc catcatgcac acattgattt
    13261 aaaataccgc caaaagttgc ttctttttca attagtaaaa tattatgaat tcctgcgtca
    13321 taagccgcaa cagcagcgcc aagtccagca ctaccgccac caacaataat tagatcatac
    13381 ttttttgcca tgtttcacca cgtaactttc tttttcgtta tacgctattt catcaacggg
    13441 aatcccgtaa tattcactca taatcgcaat aacttttgcc gtacaaaatc cgccttgaca
    13501 taaaccaaat cctgttcgta aacgcttttt catcgcttta acgctgttaa ctggtaaagc
    13561 gcggctcagc acatcaataa tctcggcttt tgtcaccttt tcacaattac agacaagtgt
    13621 cgcaaagtct ttattttctt taacttttgc ttgaatttct ttagtgctta agcgttgtaa
    13681 acgaatcagc ggtctaacgc gcggattaaa attagctttt actttaagcg gtaataaatc
    13741 tttcactaat tcattaacga cataaaggga aatcgctggg gcacttgcta aaccaggact
    13801 ttcaatacca gcaacattaa ttaaaccttt aacatattta tcatgttcaa taataaagtc
    13861 accagtcgat ggtgttgccc gtaacccgct gaaagttcga attgttttat ggaacggaat
    13921 atcttttaat aaccgattag cgtttttacg gataaattca agtgaagcat aatccgcccc
    13981 aacatcaact tttgacataa tttgttcact tgtcggtcca atcagtgtat taccagcggt
    14041 tgttgggaca actaaaatcc ctttactatg tatattaggc agcgggaata atactgtatt
    14101 tacaagtgga accgttaatt tatcaaggac aaaataacta ccctttcgcg gagtaatttc
    14161 aaaatcagcc ttacttacca tattaatgat atcatcggca aatacacccg ctgcattaat
    14221 cacaacttta ctaaaataat caccattctt cgtcgtaact ttaaagccgt cttcaagctt
    14281 ttttatcgct actacctctt cattaagctt taaagtcatc ccgttttcaa ttgcattttc
    14341 aatcgcgtgt gccgttagtt caaaagggtt tactatccct gctgtcggtg caaataagcc
    14401 gccgacaata tgatcatgta aatttggttc aagagcatgt acttctgctt tatttaacat
    14461 tttgacttcc acgccgttaa gctcagcgcg tgcttttaat tcatcaagtc ggggcaattc
    14521 ttcttcacct tgtgccacaa ctaaactccc aatccgctta aattccacat ctaagtcact
    14581 acataaatct ttgtaaagcg gcaccgaagc aacattaaat tttgctttta atgtccctgg
    14641 ctttgggtca taaccactat gcacaattgc gctattagca gcactagtgc cattacctac
    14701 atcgttatct ttttcgagca ttaaaatatt aagttcatat ttacttgctt cgcgtccaat
    14761 taaagcgccg attaccccag cgccaataat aataatgtca tacataaaaa ttcaccttag
    14821 agcaactccg tgcttttcta tatatattta tcatacacta attataaaaa taattaggaa
    14881 ttaaagcctt aaattattta catctattaa aaaacctcca aaccatatgg ttaagaggtt
    14941 taaagaattt tcaatagtaa ttactattta ttattacggc gaccgccact tttacgttcg
    15001 gtgtatcctt ctggtttagc tttaaagact tttacactcg ctttaacttt acctttttca
    15061 tcaacaccag taactttaac ttttaatgta tcaccaattt tatataactt tgatgcatca
    15121 tcaacataag cccagtctaa atcactaaca tgcactagtg cgtcaacatt accaaagaga
    15181 ttaacaaaga caccataagg ttcaacgcgg gttactttac cttcgtaaat ttccccagcc
    15241 tttgcttctt taacgattgc tttaatcgtg gcaagcacat tattaatcgt cgcacggttc
    15301 atgtgataga caacgactaa tccgtcgtca tcaatatcaa tcttcgcgcc atcattatct
    15361 tcgataatct tattaatgac tttaccgcca gtaccgataa cttcacggat tttatcaaca
    15421 tcaattgtca tcttacccat ctttggtgca aattcagcga catcttcacg tggtgcacta
    15481 atcgcactta attgatttgc gcgaatttct tcacgagctt ttttcgcttg cgctaagctt
    15541 tcggctaaga cttctttgct gattccgcgt actttaatat ccatttgtaa tgcagtaatt
    15601 cctttactag taccagcaac tttaaagtcc atatcaccga agtgatcttc taagccttgg
    15661 atatcagtaa gaatggtata gggatgatta ccatcaagtg gtccacttgt aattaagccc
    15721 atggcaatcc cggtaaccat atctttaatt gggacaccag cggccatcag tgccatactt
    15781 gaggcacaaa tactagcttg tgaacttgaa ccattacttt caagtacttc acttacaaca
    15841 cggatggtgt atgggaattc ttcgtcactt ggaataacgg caagtaaagc tctttcacct
    15901 aaagcaccat gtccaatttc acgccgtgct ggaccaccta atcgtttaat ttcgccaacg
    15961 ctataaggcg ggaagttata atggtgcata aagcgccgtg attcttcatc agttaagtta
    16021 tcaataattt gttcgtcact ctttggccca agcgtcacaa ctgataagac ttgtgtttgt
    16081 ccgcgggtaa aaagtgcact accatgcgtt cttggtaata agtcaacttg actatcaagc
    16141 ggacgaattt catcgacttt acgaccatca ggtctaactt tttcgtgagt aataagccgg
    16201 cgaatttcat cgctcaccat tacttcgtgt aacgctttaa catcacttat aacacggcgt
    16261 ttgtcgtcat cactttcata ttccttagct tcatattctt caaccatttc gcctttaaca
    16321 gcttcaattg ctgcactgcg ttctaatttg tcaaagacac tgatagcttc agtgagttta
    16381 cctgtatatt tttctttaag ttcagcgact aaactttcat caggtacaaa tagtgggaat
    16441 tcgcgtttag cgacaccgat atcacgctga atttcttctt ggaatgcaca aagttcttta
    16501 attgcttcgt gcccaagcat taaggcttca agcatatctt cttcgctaac ttcattagcg
    16561 ccagcttcga ccatgttaat agcacgcttt gtaccggcaa ctgtcagttg taagtcactt
    16621 ttagcgtttt cttcggtcgt tgggttaata ataaatttac catcaacgcg accaacttgc
    16681 acaccagcaa ttggtccttc aaacggaata tcactaatcg taagcgctaa actgctacca
    16741 aacattgctg tcatttctgg agtgcagtcg taatcggcac ttaagacagt attaataact
    16801 tgtacttcat ttctaaatcc ttccgcgaat aacggacgaa tcggacggtc aattaaacga
    16861 ccgattaaag tagcgtgttc tgtttcacgt ccttcacgac gtaaaaatga accaggaatt
    16921 tttcccgctg catacatttt ttcttgatac gcaactgaga gtggaaagta atctaaattt
    16981 gtcggattat ttcccgcaac agcagtactt aagactgccg tatcaccata gcgcactaac
    17041 acagcgccat cagcttgttt ggcgaattcg ccagtttcca cgacaatttt acggccatgg
    17101 aacatcattt cataaacttt tttcatcttt tcctcttttc tccaacataa ataatgaaaa
    17161 aactgtcata gacagtttaa tcttttttaa cgacgtaagc ctaattttgc aattaaagct
    17221 aagtaacctt cacgattaac acggttatag taatcaagaa gaccacgacg ttgaccaact
    17281 aatttaagta atccgcgttg tgaatgatag tcgtgtttgt ggactttcat atgttcagtt
    17341 aattcgcgaa tttgttcagt taataacgcc acttgcacag gagttgatcc tgtatctttt
    17401 tcattcgcac cgaattcttt aacgatttcg gcaatgcgtt cttttgttaa tgccataact
    17461 ttattcccct ttctctttat tcttaacttt agcatcgccc taattctagg ggtgaatcat
    17521 agcagcgcta aagtcgctac ataatattaa catattactt ggtaaattgc caaattaaat
    17581 atgcttaatt atttatttaa ctctgctttt attttcatga tgaaattacg agcgtagagt
    17641 cgagtatctt ctaattcttt ttcactaggt tttaagcgca ctttaaagcc ttcatcaact
    17701 ttcttaaagc gtaattcact tgcgcggttc atataattac gcaccgcttc cccactccag
    17761 ccataactcc caaaagcgct aaaatattta ccaaagcaca tcaccggatt aagactattc
    17821 aagacatcat acactggttt aatcccgtcc ccgacaaagg ttggtgaagc aactaggagc
    17881 gcatgacttt cttcaatgcg tgccacaatt gttgctaggt catcacgagg tacttcaaaa
    17941 acatgaacta aggcactcgc tttttctaat tcactaataa cggcttcact catactcctc
    18001 gtatacccat aagcactagc aaagactaca gtaaccgtta atttttcact tttatttaca
    18061 gttgaccatt taatatagtt attaatcgcc caatcaacag cttcttttgt gtctaaaata
    18121 taaccgtgac tattaagcac catacttaca tctaaacttt ttaaagtcgc cacactttta
    18181 agcacgtcac ttttaaaggg tgccataatt gcgtcgtaat atagtttaat ttcttcttca
    18241 ataatctttt tattaggttc tttactataa agtaattgtt gtggagcaat atgttgccct
    18301 aaaaagtcac aagtaaaagc aatcgcgtta actgggtcat gggtaataat agtatccggc
    18361 caatgaacgt ttggcacagg gtagaaaaat aaactttgcc gctcactaag tttaagttct
    18421 tgttttggtt taattacatt actaataaat ggtgcattat taatttcttt taaattagcc
    18481 attgccataa atgaaccgta aacttcaata tttggattaa gcgctaaaag ggcaggaata
    18541 gcgccgctat ggtcaggttc agtatgttgc acgactaaat atttaatatc tttaatatcg
    18601 actaatgaac gtatattgtc taaccactta tcagtaaatc ctgctttaac tccgtcaata
    18661 atcgtcactc caccttcttc tttaattaaa taagagttat atgttgtgcc gtgttttgtc
    18721 tccatcataa tatcaaacac acgaagtcct ctatcactaa ctccaatata ataaacatta
    18781 ttttttacat taaatacttg catttaatat tctcctttct ctatcttaaa ttcacaattt
    18841 tcacgatctt tttgtaattg cttaatcaac gtgtcaacac tttcaaactt aaattctgga
    18901 cgtaagtaat caataaattc aacagtgagg gcgtcgtgat aaatattttc attaaaatta
    18961 aatatattaa cttcaagtag tggcacatta actttgttaa ccgtcggatg gtaccctaaa
    19021 cttgccatcc caaagtaata attagctcta aacttaacgc gaacaaaata tacaccgtgc
    19081 gctggtacaa agtaacttgg gtcaagggca atattcgcag tcggaaatcc taatttattt
    19141 cctaaaccgt agcctttagt cactaaccca gtgagcgcat aatagcggcc aagtaatgac
    19201 tttgcgatct taggtttacc tttacttaaa agctctttaa ttagtgatgt gctcactttt
    19261 tcattttcat agtaaagtgg cgctaaaact ctagtcgtaa atacattttt accaaactct
    19321 cgcaaatctt cgccagtgcc tttagcgccc cgaccaaatc taaaatcagt accaacaata
    19381 acagaatttg gagctaaagg tactaaaact ttattaataa attcacatgg ttcaagcttg
    19441 agtaattcat cacctaaatt taataaataa acagtcttca ctccaaactt ctcaaagatt
    19501 tcaacacggt cttctaaact cgttagcacc ccactttttt gatgaagttt tgtaagtttt
    19561 ttattagtgt atacaagtaa aaccgcaaca ttatctgccg ctttaaaacc ttcttccact
    19621 aaatacttat gaccgatatg gacgccgtca aacgtcccaa caactagtgc agtgcttggt
    19681 gctaaagatg taatacgact taaatcaaga tgtttagcaa tcataaatta aatcctcgta
    19741 gcgcgatata attatcgcct tttttcttat ataatgcttg caaaacgcca ctattattat
    19801 gaatttgtac aatttcttca ctagcattaa gtttaatcgt catcccgttt cgcacccttt
    19861 taacttcctc atcacttaac ttataaatag gaaacttttt taaccctaga cttggatcaa
    19921 tgatatcttc ttcacttact tcttcaaaaa gttttgcctt ttcaagacta aagtcaccat
    19981 gccttgtgcg cactagctta atcgttgtag cgtttgttcc taaggcaagc gctatatctt
    20041 gagcaagcgt tcgaatataa gtgcctttac taacagttgc ctctatgact agtaaaggat
    20101 gattatattc aagtacttct atagtgtaaa cttctactac acggcttggc atgtcgactt
    20161 caatattttt acgcgcatat tcataggccc ttttcccatc aattttaatc gcacttgtcg
    20221 tcggtggaat ttgtgttact tcaccagtaa attgcttaat aactttttca atttccgctt
    20281 tagaaaaggg cggaacttct tcttttttaa taacttcacc atccggatct aaagtcgttg
    20341 ttaataagcc aaagtacaat gtcgcaacat aagttttaac ttcatcttct aaatacggca
    20401 acactttcgt gccttcacca acagcaaaga tgagtaagcc actagcaaag gggtcaagtg
    20461 tccctaaatg cccgactttc tttatcttaa gtttacgcga aagcatgtta ccgactttgc
    20521 gactcgtgta accaggctct ttattaacta ataaaatacc gtttttccct ttattcatat
    20581 atcttttata ttataatata aagcaacagg ttttaaaaca atgaaacatt ttcgcaaatt
    20641 actagcaaag attaagaaag ctgatgagaa gtttaatcta attcaaagtg gtgacaaaat
    20701 tgtcatcggc atcagtggtg ggaaagactc cattgttctt tttgaagcac tacggactta
    20761 taagcgttat agtaagaaag actttacgct ttaccctgtg attttagatt taggatttag
    20821 cgggtttgac gctagtagtt atactgcttt ctttaaggaa agagaatatg acttaatcgt
    20881 taatgattcg cgtgacgtca gtaaaatctt aagtgttcag aaagaaaaac aagggttatc
    20941 aaaactgccg tgttcaatat gttcacgaat gaaaaaagca gcgattaatg aagtcgctaa
    21001 taagttagga gctaataaag tcgcctttgc tcatcatatt gatgacgcgc ttgaaacact
    21061 ctttatgaat atgattggtg gcggacgctt cgcgactttt agtccacaga tgtatcttga
    21121 aagagcggaa atcaccttta tccgtccact tattcttgtc gatgaaaaac ttataatccg
    21181 tgtgcaaaaa gatttaaagt taccactcct tccttataac tgtccaaatg aaaataaaac
    21241 aagacgcgat gacattaaaa gtttattaaa cgcgatttat aaaaagtatc cggaagcgaa
    21301 aactaacttt gaactgatgt taattaacga caaagaattt gatttatggt acaaagatta
    21361 cgcaacgcta ataaatgatg actgtgtcat tgttaaagtt actactcctg cacactttaa
    21421 tgatgccctt tatattcgtt ataaagtatt cgtcgaagag caaaacatta aagtcgaaga
    21481 cgaatatgac acaaatgaaa aagattacac tagtttagtg ctctatgtta aaggtaataa
    21541 agcggccaca attcgttata agtacgacgc actagcaaga agagcgtact taggtcgtat
    21601 tgcagtatta aaagaatatc gcggtcttaa actaggaatg actttaataa cttatgtcga
    21661 gcaaaagtta agtgttaaat atcgccccct tactttccat atcgggggtc aaagtcatct
    21721 attaaaatat tacgaaagct tagggtataa acgcgttggt gaaagttata tggacgccgg
    21781 tatcttacat tatcactttg aaaagaaagt tatttaaaat tagacacttc ttgtcaatta
    21841 aaaataaaat aggcctttat aatcaggcct atttttctta ttaataatta actttacttt
    21901 ttagtacgct ttaataattc atctaatttc gctactcttt caaaagtatc gtcataaata
    21961 aagcgtagtt ctggtgtctt tctaatattc attttttgcg atagttcatg gcgaataaat
    22021 ccgcttgcgt tatttaagat ttccatcttc gcctcaacat cgccatcccc taaaaaacta
    22081 atatatactt ttgcataact atagtcacgc gtaagttcaa ccgcactaac agtagcaagc
    22141 cctaaacttg ggtcacctac tttatagcta ataatttcac taagatgacg gagcattaat
    22201 gattcattac gtgataaatt acgactttta ggcatcttta acctccacca tcgcatagcc
    22261 ttcaataata tcgccttctt taatgtcgtt atagttaagt aaagtcatcc cgcattcata
    22321 tcccgcgcgt gtttctttaa tgtcatcttt gacgcgtttc agtgtcgcta cttcaccttc
    22381 gtaaataatg acgccatcac gcagcaaccg aaccgtttga ttacggcgaa tgacaccgct
    22441 cagcgtataa catcccgcaa tagtgccaac ctttgaagcg tgccaaattg ctcgtacttc
    22501 ggcttggccc gtaacttttt cttcaaattc aggttttaat aaacctttta gtgcttgttc
    22561 agtttgttca agtaaagcat aaattacgcg atgtaaatga atttcaattt ttagtcgttc
    22621 tgcaaggtca cgcacttggt tatttggacg aatattaaac ccgaaaataa ctgcattact
    22681 cgcacttgca agtaagacat cactttcggt aatttggcca gcacttgcac gaataatatt
    22741 aaggcgcaca ccttcgacat taatcttttc aagactcccg cgtactgctt ccgcactacc
    22801 gctattatca gccttaataa tcacattaac gacaacagtt tcaccttcgg caatttgctt
    22861 atggagatca ctaagtttca tcccttcact actaccgcgg ctctcttcaa tgcgaatttg
    22921 tttgcgttta gtagcgagtt cttttgcttc tttttctgtt tcaaaagcga tgaaattgtc
    22981 acccgcttca ggtacttcgt caagaccaat aatggcgact ggtgtcgctg gtccagcact
    23041 ttttaatgcc ttattatgct cattagtcat ccgccgtact ttaccataag ctgctccggc
    23101 aacgacaaaa tcacgatttg ttaaggtgcc gttttgtatt aaaagcatag cttttggtcc
    23161 ttcaccgcgt tcaagacgcg cttctaaaac tgtgccgtag gcatagcgct ttggattagc
    23221 ctctaattca agcatttcag cagtgacaag aatcgcgtca agtagttcat caatacccgt
    23281 tccttttttc gcagaaattg gttgaaaaat aacgtcacct ccgtattctt cagcaatcac
    23341 gttttgcgct aaaagttcat ttttaacttt ttcaggatca acacctggct tatccatttt
    23401 attaatcgcg acaataattg gtacttcggc agcgcgggcg tggtcaattg cttcaatagt
    23461 ttgcggcatt accccatcat ctgcggccac aactaaaatg acgatatcag ttactttagt
    23521 cccgcgacta cgcattgcgg taaaggcttc gtgtccagga gtgtcgataa atgtaatctt
    23581 tttaccatga aattcttttt gataagcacc gatggcttgg gtaatactac ctgcttcgcc
    23641 actgacaatt gatgagctcc ttattgtatc aatcagtgtt gttttaccgt gatcaacatg
    23701 acccataatc gtcactactg gtggacgcgg tttaaggtct tttggatcat caacaatatg
    23761 cgataatcca aagtcgcaat catcttctgt ttcttcttta aaatcgtaat caaagttaaa
    23821 gtctaaacat agttcggcca ctgtttcagg gcttaacaca ttattaatcg tgaccatttg
    23881 ccctttcata aataaattct taataatttg cgcctcacta agttttaaaa gccgggctaa
    23941 atcgcttact ttaatcgggc cttgataaat aagttttctc ccctcaacac aagggccact
    24001 ttgtttttta ctgtggtgtg caacattact gatgcgcttt ttttcattac tcatttcagt
    24061 taccttcttt cattagattt gcgatttttt gagcgctttg atggttagtg acaactaaga
    24121 ctttaattgt tttaccagga taaagcatag acatgatgtt cgcagggaca tcaaattgtg
    24181 cgatgttatg cgctaaagtg aagttgttca ccttagtctt tgtgtactcg cttaagtcat
    24241 cactagttag cactagtgcg attttacgag cgttactttc tcttaagtaa tcagcgccaa
    24301 agcgataatg gccactgcgc tttaagagat gaaggaagtt attaagttcg ttagtcatta
    24361 acttcctcaa ggagtttttc gtatataact tggtcaactt caatgtttaa ggcttggctt
    24421 aaggcatttt tcttttgcgc taagagaata acttctttat tgcgcactaa gtaagcaccg
    24481 cgaccattga cttttcccgt attatcaatt accacacgtc cgtctggcgt tcttacaaca
    24541 cgaataagat cacgtttttc gtgactttca tgggtaacaa tacagcggcg aacgataatt
    24601 ttcttcattt acacactcct agttatcgta gtattcatcg aaatcttcaa agtcgagatc
    24661 atcgtcttca tagacttctt caagttcagc gtcgagttct gcaagttctt cttccgtata
    24721 aatatccatc tttggtccag tcggaagtgt tggttcaaca acttcaggtt gcttaacttc
    24781 ttgtttagtg cgactacgac gttcttcgcg tttttgttgt gcttgttcaa tttcttcttt
    24841 ttctttggca agttctgctt ctaagtcagc aagcgaaatt gttgttttaa ctttttgcac
    24901 aactggttct ttcttttctt ctttaatttc aggagtgacg acttttggtg tttcaacgac
    24961 tttttcttca tctttgaaag catcttcata ttctaagtca tcaatattaa gggaattaac
    25021 ttcctcttca agcaaactct tttgactagc ggcgcgttgt gcaacttctt cggcacgcgc
    25081 aagttcgcgt aaaatacgtt cgcgtgtccg ttcttgttca gcgagccgtt cttcgtattc
    25141 aatatccgct tttgttaaga attcaacacc ttgttcacta gcttcattca aatcaagaat
    25201 cgttaggtcg tagccagtta atttactcgc taaacgaata ttcgcgcctt tatgaccgaa
    25261 ggctttacct ttttcttctt cgctaaagac gacttgtgct gttttatttt cttcgtcaat
    25321 ttttaagcca agcggcacag ccggacggaa ggcttcaata atgaagagcg cttcgttttt
    25381 aacccatggg ataatatcaa tcttttcttt acggccatca ggaccaagtt cagcagtaat
    25441 tttttgaatg cggcttgcat ttggaccaat acaggcgcca attggatcaa catttggatc
    25501 aagactttca actgcaactt tgacgcgttc accttcttcc cgcacgatat catgaataac
    25561 gatagtacca tcataaattt catgaatttc gcgttccatg agccttttta cgaagttagg
    25621 atggctgcgg gaaataataa tatttgcccc tttagcgcca ctaacgacgt caacaatata
    25681 gaagtttaat tcttggccag gtttaaaaac ttcaccagga atcgcgtgcc gtcctctctc
    25741 atcaaagaac acacttgtaa ttcctaataa attaatataa gtaattcgtt catttttctc
    25801 cacggtacct ttaacaagtt caccaatttt atctttaaat aaagtgaaga gttgttcttt
    25861 ttcaagttca attaattgat gacggaagtt atgggcgaca ttatttgata attttgaatt
    25921 gaaatcgcct aagaatgagc gttcaacgac gactaatgca tcgacttctg catcagcgtc
    25981 aaatgtacgc gctacttcta aacatacttc aagtgcatca tcttcaatat cgtcatcatg
    26041 agcgacaact ctttttaaga cccgtgcttc aaaatcaaac atttcttcgt tgatcacgac
    26101 ttcaacatgc acatcatctc cacccttttg gcgagcgaca acattttgga cagcacgcat
    26161 aaaaacatta agaacaactt cttcctttaa gttgtgcttt tccgcaactt cacgaaacat
    26221 ttcacgcatt gcttcgaaac gttcttgctt ttttcttaat tgtgcttttg ttaatttttt
    26281 actcattttc gtattcctct ttaacatttc actgcgcgat taattgtttt aatatcactt
    26341 tgcgcataag tttccttttt aattcgtcct ttatcgttat gactaatcgt cacttcattt
    26401 tcactaacag cttctaggat accttttact gtgttaggac ctttgacacc atctttcatc
    26461 gttaatttaa tgaataaacc tagataatca tttacttctt gtagcgctat ttctttttcc
    26521 gcgcccgtcg ttgtaatatc aagggtgaag gcactttctt catcatcatc tagtattgcg
    26581 tcaattgctt cactgatggc gacaatttca tcaagactaa taatgtgctg gtacttatca
    26641 acgattacgc gtaagaaacg tgtaccttct tcattcaaga aacgcatact tactaagcga
    26701 taaccgcgtt cctttaatag ttcttcgact tttggttgta ataagtttaa atccataatc
    26761 ctcctgtaaa caaagagtgg gcgtacccac tctctagagt ttgaattaat ttgtaagtaa
    26821 aacttacagt atcataatag tagattttgt acaactttgc aactaaaaag tgtcaaatta
    26881 ctataatatt agcgattatt tcatatttac accttaaatt tacaatgatt ttttcttaag
    26941 aagtacacga tccaataccg tcgcaatagt aaaaaagact tacttaacca gtactgtttt
    27001 gattttcatg ttttgttttt cattaataat tctttaattt tactttcagt atcatttgtt
    27061 agttcaagtt tagcaatttc ttttttaaat atcatttcta attcatttac acttaattta
    27121 tatacttttt caaaaccatc aaaaatatta tctttatacc tgtaaaagac taaatatttt
    27181 ttaattccgt agtgcactat aagataatta gtaaaagcac ccataattgg ataagttaat
    27241 gaacaatctt cactaaaaaa gtacttatta tcaagtaaat tacaaattga aatatattta
    27301 tttgttttta agtagtatag tgtccagtct tcattactta tcccccacca tacacggtca
    27361 aaatacattg ctagtccttc acgaattgcc gcgctttcgg gacgatttat tgtgtaacta
    27421 ataatatgag catcttcatg aaaaccaata cactgaatat cgtcattata taccgcataa
    27481 attgagtcag gcgaactagt aaaagcgtta cacggttcat tgtcaccata tatcttccca
    27541 acttcttcag gcgaattaca taaaaagtat ttaattttaa acttagcctc aacccctaaa
    27601 actttacaaa tatgtgtgta gcaagcttgt tgatgctccg cgattaaaag gatatcttct
    27661 gcagctttac tacccgcata gtaattaaag atatagtgtt tgctttcatg ttgtaacatt
    27721 aagttttcgt ttcccatttg tcgtaaactt ttaaggcgtc ctttcgcctc atcttattgc
    27781 tcataagtta gtgctttaat tcttagctaa ttatactacg ataaacgctt cttactctta
    27841 tttagttaac aatctcttaa ctaatagtat tatttaaact ttattaaata acatcactac
    27901 ttgacttaat ttgcgaaatt aaggaaaata acgctggtga tagccgctga ttacgcttat
    27961 tgggagctaa tcactccaga gcccttggac tgttcaaggg cattttttaa attctaacac
    28021 tttccaatta ttaataactt atagtaaatt aaaattaatt agtcatttaa tttgtcatta
    28081 attggtaatt gaattgactt aaatgaaaaa aataggtaaa ttaatattgg gcatacgatg
    28141 taatgcgcga attaagtttc gactcatctg tgccttttta ttttgcttga aataacaaat
    28201 aatctaactt aataattcgt tagtgatatc gactctgtgg cgaccaaagc ccaattaagt
    28261 aagttttaca ctaactattt tatttagata attattattc cgattaccaa caatttgtat
    28321 atcaaaatac agcaccatct ggaacacaat gaataccaat aaaatcacta tctgtcacta
    28381 ttatttacat ataatccact aattttaact tttatagtaa atccatttct aaacttgata
    28441 ctgttatgta ctgataccct aaatgtagac acacaataaa atcatgtggc cacatgattt
//
LOCUS       DAOSLP010000010        27002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_1983_length_27002_cov_8.160426, whole genome shotgun sequence.
ACCESSION   DAOSLP010000010 DAOSLP010000000
VERSION     DAOSLP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 27002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27002
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1983_length_27002_cov_8.160426"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1685)
                     /locus_tag="PK460_01795"
     CDS             complement(393..1685)
                     /locus_tag="PK460_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53865.1"
                     /translation="MNKYEDKLKKIKKDIPEYPKTLDPKVLEAAHKAPRSKRPGFPKL
                     LIPSLLTVTAIALSTFFVTKYVYYWNNPVNSSSDTSDPTDKSSEPTNRDFAVMKSLSI
                     DGTSLDPDVIGEGRYGYMDIIFVDSHNSLNMTITKYAEEAQPTLNDLTFEIKDNDDFF
                     DDNVLLIVPFAHTSSETEISLEGLDIAGNNEPTKIHFDIFSPEFNDMDVLVQFNVVVI
                     GTWASESLNKGLFEIIVHNTLDNSNKSVFYGHNSITNDNSETPSEKENEESIQQSFIV
                     KKSLSIDGTSLAPDVTTEGRYGYMDIVFVDSLTTLNATITKYAEEAQPTLSDLTFDIK
                     DDDDFFIDNVLLIVPFAHTSTEIDISLQNVYLSENEEKTKLHFDVYSPEFNDMDVLVQ
                     FHIVTIEKDYEVFLNNSKFKIIVNNTFDNSNKSVFYRD"
     gene            complement(1678..2142)
                     /locus_tag="PK460_01800"
     CDS             complement(1678..2142)
                     /locus_tag="PK460_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE53866.1"
                     /translation="MTNEQFAIVYETYKVMVFQIAYSYLQSVEDAEDAVLESFMRLLR
                     INKNFVSSDDERRYLTRIVVNVSKDMLRKKRPVSISDPIVAISDEPLNENEYIWDEVN
                     KLKTKYKDVIILRYVNEYSFSEIAKVLKISASAARKRHERALKMLKEELEHE"
     gene            complement(2573..4126)
                     /locus_tag="PK460_01805"
     CDS             complement(2573..4126)
                     /locus_tag="PK460_01805"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOE53867.1"
                     /translation="MNLEDALKRIAILEKENEALQLENEEQRLLIFKQQQKLNELLKD
                     KEIVREKLIIERTKPFVAKSEKINEIVINEPEHILQENKHTKARVGRKKGGKNFANVD
                     LEAAVIDTVYEDPDSLVCPACSNDLTLASQKERYIIEVIPSTIKVTKVIKRSYKCKHD
                     QTFVYPLSQEVFPGSVLTPSFAAYIAYHKYELGIPFHHLEKHLTNVLNVPISKQLMAT
                     WMQTLATKLTPVYNAMKRDLLSNSVKVIHADETTLSIAKRPENALNRKKSYVYVYASS
                     YFDQQIHLYDFHESRSIDLTAKWLSKYDGYIVCDDYAGYTKLAKNNQNIKLQRCWAHA
                     RRRFSDILKALPTTEVKTTLSYKILTLINELFAYEAKYKTDAVLPSEICAKRHHDQIP
                     LLNELKTYVFETPLSPNSALEGAVHYVRKIWDELLTYLDYPYLEISNNLAERAVKPFV
                     INRKVFMTAGSYAGAKYTTIIFSIIRTALINHLNIEKYLIYLLENLDKQPLDELLPYA
                     PKLPNKLKV"
     gene            complement(4197..4532)
                     /gene="tnpB"
                     /locus_tag="PK460_01810"
     CDS             complement(4197..4532)
                     /gene="tnpB"
                     /locus_tag="PK460_01810"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOE53868.1"
                     /translation="MIDLNKVRNIFLYTNKIDMRMGIIKMESILSLSFSPLEIHRSVF
                     IFVSKSRKMVKIYYEDEYGKWLFINKLSYARFNVPALEESFTITKDDLAYLLKGVALI
                     SERRKQVSL"
     gene            complement(4529..4897)
                     /locus_tag="PK460_01815"
     CDS             complement(4529..4897)
                     /locus_tag="PK460_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE53869.1"
                     /translation="MGKYKTDYERLETVKEFKMSKLSVSEFARQKGINRTTLRDWINA
                     YDNIDGSFVRLNKVLDSSRKSVLLSNDDVNVSLLKPEEIRAKSKNFTRFDHSVVVIEV
                     AQVKVTTSLQQALAILEKIL"
     gene            complement(5304..5954)
                     /locus_tag="PK460_01820"
     CDS             complement(5304..5954)
                     /locus_tag="PK460_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53870.1"
                     /translation="MKKLLTSFLLGLLLLAGCENTRKYRFTIIDTDRTVLSKHKDRYS
                     AGEAIRIETVMLTDVIVSMYVNDEFYNNATTAENNVGQSVFVYDFIMPTYDVTITFVI
                     KDGMIVEPNYIDITFIQRKIVDWQNFEDYLIPVFGEEDLTITTLSFEDNIKLTLEEID
                     DLQSRSVLNYIPPETMYGYYIFYGFYFDEENTNDLHRLTPEFAVTLYNTYYYGLAG"
     gene            complement(6014..7042)
                     /locus_tag="PK460_01825"
     CDS             complement(6014..7042)
                     /locus_tag="PK460_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53871.1"
                     /translation="MKRTQILVAGLLLILTSCRAANHQTLSEVFPMLKTEEFAEVKHT
                     ISCNSLAPLALINHYYNDDVAAVTNFVNELGKTKVRRYAKLPESKNVACPTINTYAFK
                     NEALEFTFDVTNMFVVKDDVYYELEELPNIVTTYSSYSFSGSTTITEVFNSEGKEITD
                     FSLSLPSLEFKELTELSGNNVNFTIETVGGIISVYDAVTFGLNVGEQIERFYEVISGD
                     TFANLFDTNNNFTDDTMLLANIPQQLTFRNQKYKMITSGFFEWQDIQLDLLIAYFVNE
                     RDYDVFYLENPDIEYCIDEANTLYYAAGPSKFPLYSLVGHAIEQLLGLLLPQGGGSPM
                     IFLNISCV"
     gene            complement(7053..7817)
                     /locus_tag="PK460_01830"
     CDS             complement(7053..7817)
                     /locus_tag="PK460_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53872.1"
                     /translation="MNNHEEKLKKLKNDIPEYPDGLDQKVFRAAEQAPRYKRPGFPKL
                     LIPSLLTVTAVVLSTFFVTKYVYGWNTPSNTPSNSGMPSEPSNTSAEEGEVYDYYVSA
                     SAAIPGASLAPEMLVEGKLGYFDIVYIDSQLALDATINQYADDAHPVLSTLTSVIRNN
                     AEFFSESVLIIVPFAHTSTEEGIELTQIEFGSKEQPTKLRFDIYSPEFNDMDTLVTFH
                     LVTIGEWASDLLSESAFKISVHNTFDNSNKSVFYSN"
     gene            complement(7810..8274)
                     /locus_tag="PK460_01835"
     CDS             complement(7810..8274)
                     /locus_tag="PK460_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE53873.1"
                     /translation="MTNEHFAIVYETYKVMVFQIAYSYLRNVEDAEDATLESFMRLLR
                     INRKFASGDDERRYLTRIVVNVSKDMLRKKRPVPISDPVIAVNDAGQNENNYIWDEVN
                     KLPNKYKDVIILRYINEHSFNEIASVLKISASAARKRHERALKMLKEELSHE"
     gene            complement(8592..9938)
                     /locus_tag="PK460_01840"
     CDS             complement(8592..9938)
                     /locus_tag="PK460_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE53874.1"
                     /translation="MIFKRKIYSDLLNWKSEGGKTALLIEGARRVGKSTIVEEFAKQQ
                     YKSYVLIDFSIASDDVKSLFTNYASSLNDFFFFLSSLTGQKLFERETLIIFDEVQLFP
                     AARQLIKHLVKDNRYDYIQTGSLLSLKQNVAEILLPSEERTIKMYPLDFEEFCWATGH
                     NDLIPFLQKRFEERKPAKELHQLAMKLFRQYMLIGGMPQAVAMFLETNDYTRVDRAKR
                     DILQLYRNDIRKYAKNNEYKVISIFDEIPNQLTKHEKKFTLSALSKDARFRDYEEAFV
                     WLNEAMMTNPCFNASDPTVGLGLYRESLTLKMYMGDTGLLFSHAFNENEIMQTEVAQS
                     IVAGKLNINSGMFLENIVAQMLVASNKRLYFYSRNDRKNAKNTMEIDFMISDPHNPRK
                     ISLVEVKSGKNYTTSSLKKMKVKFGNKLGQSYLLHTKDLRVHEGVIYLPVYMTIFL"
     gene            complement(10498..11856)
                     /locus_tag="PK460_01845"
     CDS             complement(10498..11856)
                     /locus_tag="PK460_01845"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOE53875.1"
                     /translation="MRITMKEKVKICKMHIEEGLSISHISELYGGYDKTNIKYIINLY
                     KMHGEEPFLSRVRRMYKRDTKLLAIGQVLAGRTIRDVACEHGLANPGVLSDWLKKYRE
                     GGEEAIQDTKARANYLLKEDRFKAVVDRKIEAENERLRAEIAYLKKSQSLAQKLEGAT
                     VQERVRIVDELRTKYKLSILLELSGLSSSVFYYTLNANKTRQNKYVELEKEIERLFIK
                     VHKKRCGYHQIYNNLKKNGWIVGKNKVLEIMRAKGFSKPKVIKWRKYNSYEGNLGLKI
                     ENIMNQDFKTTRPYEKGGTDVTEFPMFGESVYLSPVIDFHTREVLAYSVGTDAKVYRI
                     IRMLRELQKKHGNNLMGMILQSDQGVQYQNSRYIVELRNMGIIQSMSRKGNCLDNSPT
                     ENFFGRLKQEIWDNNKRDYDSVEALITTIHEYINYYNKTRIIMKTKLTPIECRNNYLL
                     SI"
     gene            complement(11933..12006)
                     /locus_tag="PK460_01850"
     tRNA            complement(11933..12006)
                     /locus_tag="PK460_01850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11972..11974),aa:Thr,seq:ggt)
     gene            complement(12138..13178)
                     /locus_tag="PK460_01855"
     CDS             complement(12138..13178)
                     /locus_tag="PK460_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOE53876.1"
                     /translation="MKIRRLLLALIGVTLLASCEPPTSQSIEVPESEIPSVSDISDTP
                     SIVSETSTTEEVTSVEESSEEVSSEWVEPEPVLKPAENWNFRPLPAYTGNFWNTIDFT
                     LRGEELRQALADYMWSKFYVPGVLDNRVTYDDALEAIKVMDADPNKTGNVLSLYDLRS
                     HPHAGYPGKWNREHVFPQSKLADGDKSLRASGNTKNISSDIANLFACDAALNEHKSNH
                     SYIEWNYAEDPERWFPYLSRNTAGQLVDAIVHYGYSPTPIVRGEIARAQLYMVLMYPD
                     RCSISENFQIADMIRWDQEWAPTIERDGQRQKGIEQYQKIRNPFIDNRNLSCYIWGNT
                     NRATRKLCADIY"
     gene            complement(13245..15362)
                     /locus_tag="PK460_01860"
     CDS             complement(13245..15362)
                     /locus_tag="PK460_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE53877.1"
                     /translation="MNFNPFPNYEQDEKILEKFGRFLDEDAKLNKSDPVIGRDSEIRR
                     IIEILARKQKNNVILLGEPGVGKTAIIEGLAQRIVAGDVPKNLADKRIYELDMGALVA
                     GAKFRGEFEERLKAILKKIKESNHQIILFIDEIHLIVGAGKAEGAIDAGNMLKPMLAR
                     GEIHTIGATTLDEYRTYIEKDRALERRFQPLIVQEPTLEDTISILRGLKARYEAHHGV
                     KISDNALVSAAVLSDRYITERFLPDKALDLVDEACALIRVEVESLPAELDEIRRKMRQ
                     LEIERVALSKENDDLSKVRLQKVNEELENLKKEDEAFSKEWQHELDELNVLGKAKKEL
                     EQARFNLSVAFNSGNYEEASTLQYKVIPELEAKIETLTKETKDNKVLKETVTENEIAA
                     VVSRATGIPLKRIMESQKEKILSLYDELKKRVLGQDVALRLVSDAILRQRAGLNNPNR
                     PIGSFLFLGPTGVGKTEVAKSLAYALFDDERHIVRIDMSEYMEKFSVSRLIGAPPGYV
                     GYDEGGQLTEAVRRSPYSIILFDEIEKAHPEVFNLLLQLLDDGRLTDSQGRVVNFKNT
                     VIIMTSNLGSEFFLKNQTSKIDDLVKATFKPEFLNRIDEIVYFLPLSKDTQRQITRKM
                     LDELMAHVAKNNFKLIYDEKIVDFVLDNAYAPEFGARPLKRFIQKSLETFIAEQVVQG
                     NIDSSITYRLTVTNNEVSISPFA"
     gene            complement(15472..16497)
                     /locus_tag="PK460_01865"
     CDS             complement(15472..16497)
                     /locus_tag="PK460_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53878.1"
                     /translation="MGTNSKFNLGINLTGDSDLDRHNIFNYIIENFTKDKTEAEILVL
                     AATFLGKDTKTIFNEIRVNPEVLVPVCLYLMSEEKIALSRRNEIGNALLKYTDAHARK
                     IDDKATRKPLYNFTLEYEKALYTKMANSNVEDILVTFPFYRIYYTQGLIYEAMADYTK
                     AFVMYSKAHQWNPFNPKPILKILEGLKYDGRAQDLLRLSMWYLQITYSAPNISAALRY
                     AGYALYLDGKFAEAYAFYYQAIVYQDGETPARFNEEITSVLTALKKDAPYELSRAQIK
                     KLFAYSKFKPYPSEIAFDTIRPLIINHYTKGNYVDAIRYATQYVIFRPNDDKIMRILK
                     ASKLNLD"
     gene            complement(16564..18075)
                     /locus_tag="PK460_01870"
     CDS             complement(16564..18075)
                     /locus_tag="PK460_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53879.1"
                     /translation="MVEILVEKLLAYAELKLALNSEDINYYRNVLLNKFGLKKPYKGV
                     INVSEIQSMNNPQMLIDEMQHLIETKGLPAHLSFTDPEEVIIDVLGMMTPIPSVVSQR
                     FAEIHKKDSNKAIKYLYDLSIANNYIQEAKVAENIRWRAELSDKYFEMTINLAKPEID
                     NRETAKLLKEKQDDKYPSCPLCLENVGFGGNESTPPRQNLRAVPLTLDKEEWYLQFSP
                     FVYYKDHIIVISKDHNPMTITPRIFSKLVAFVDQFPDLFIGSNSDIPIVGGSILNHEH
                     FQGGAKVMPMFSAKDRFVLKHKHDRKLKISILEWDSNVIKLESRTKSLLLAKAAEITG
                     NWLEYTNEELGIIPATSGERHAAVTPIVVKEKHKYIMYLILRNNRTDEKYPTGIFHAH
                     PEYHNIKKEGIGLIEQMGTFILPARLKNEFGLIEELYKKGVTGAEMISEHPSIAKHAT
                     FIKELMLSKIKPTEFEKYARDYMTNVCENILQNTACFKNDEAGQDAFIKFLQL"
     gene            complement(18112..20442)
                     /locus_tag="PK460_01875"
     CDS             complement(18112..20442)
                     /locus_tag="PK460_01875"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOE53880.1"
                     /translation="MEYKYIHLKTNDTSYVLAINKYGHLENLYYGAKLALNEDLSILV
                     NKQTNMQGTTTVYSGEPHDTYSLDFVNLELGTIGKGDYRRPSILLEHELGFASDFVVK
                     KHGFAGREVLLVNDLPIPHGYDEYFYVILEDNISKVKVQLDYLIFKRANVIARRLVVL
                     SGKGKLTKAASLNLDLNNDHFDVFSTYGTWANEVHASRNKLVPGLFEVATSVGPSSNR
                     HNPFILLLNEKTDLHKGIGYGVNLIYSGPFKASVELTFDNRVRLQHGINDETFSKELD
                     GKPFITPFAALTFSDKGENGLSQNFHRFVIDHVVRYEYARKPRPILLNNWEATYLTFD
                     EAKIKKLMREASKLGIELMVLDDGWFINRDDDFGGLGNYEVNKKKLPRGLKGLSDYAT
                     KLGMSFGLWFEPEMVSADHPIFTEHPEWVVKIPSVKPSTGRHQYSLNLTLKEVQDYIV
                     ENVSKVLKSADIKYVKWDYNRNLSDYYSPSGEYGTFFYDYTLGLYAIMQRLTKEFPTI
                     LWEGCASGGNRFDLGILSFFDQIWVSDCSDAYERIRMHTNLAKAYPLSVMSGHVSAAP
                     NHQLLRDLPLQTRFNNAFMSGGFGYELDVNNLTPKDKKEIKAYNEIAKAFRSVIMDGK
                     YYELTLSSAKDKKAYALLSEASDNALLLISSGIHSPMPEVLKFPSIPVKQDLTYEVFA
                     LPQSTDISTFGSLLNMVLPKFIKHDGAVIKYLDKRSSAEELVKVEVIERYTVSGKALR
                     AGALTLYPEWTASGFSSTTRMTRDFTSVLYFITKKD"
     gene            20518..22836
                     /locus_tag="PK460_01880"
     CDS             20518..22836
                     /locus_tag="PK460_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE53881.1"
                     /translation="MKHALNYNWSFIPKFNDEYIKSIPQGVISVDLPHNMVDFPFNYF
                     DEKDFDSVGTYFREIVFTKVLESDKFVLRFGGVNNSFTLYVNSQLVGTFTRPYMEENI
                     DITKYVVEGNNLIVVCVNGSEKVNAPPFGNTVDYFPFSGIYREVTLFVYNALDIIDVK
                     VTANHLGQIEVNPDIRNINKVDYSVNYEVYEGTKLIASSIEHNFTVENPKLWSVNNPN
                     LYNLVTTITANKDVKQYVSVFGFRSAVFLSDGFFLNGEYTPLVGLNRHETYPYVGAAA
                     TSFLQFYDVVKLKNLGVNYVRCSHYPPSRAFLDACDFLGLMVINEVPGWQYIGDEAWR
                     KIHLQNIETMINRDFNRPSIIAWSIRINESADHPFYEECHKLAKALDPVRATTGTRNF
                     TNSELFEDIYSYNDFSHDGTNKGLLAKRKVTKSKKPYIVSENNGHMYPTKPFDNPLIL
                     ESHTKRHLAVLDSFFGDKNIVGVSPWCMHDYYTHHEFGSGDKICYHGVLDIYREPKPA
                     AYVYTSNLTSDPVLFPLFRGDNGDLPESKLYKTMVLSNASYIDLYKDDEVVKRYYPRR
                     DLYKHLPHPPFIIDTFISDNFKSTIKFSKRDRKKIAKLLNYAALNNFSRLSLWQKLQL
                     GWLIVKYRLKWDNLVELWFLNVVGWGDKSSGYTLVGYNDEDIEIIRKRIENTTTTTYE
                     IELLKNELVNTDTFDTTIVTIRSLSPYRNHYDFSPVSVVVTGPLKVYGPEIQNLHAGV
                     LNVYVRSINTTGRGTITIGVGGTIKTFNVEIK"
     gene            22852..23595
                     /locus_tag="PK460_01885"
     CDS             22852..23595
                     /locus_tag="PK460_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOE53882.1"
                     /translation="MAEKNLALLIDAENISPDYMSIIRDEANKYGNLTYKRIYGDWAS
                     SNLTKWRSKCIEYNLEQIQQVSYVSGKSTSDFTLVIDAMDILYSDQVDGFVLVSSDSD
                     FTRLVSRLRQNNKFIIGMGESKTPLSLVNSVDLFIYLDKIKNIRDKNVKPKKRGRPAA
                     DKKEEESIISLDDLIDVLTNIISETSDENGWAYWSNTNNTLVRKYPGFDPRNYGFKGK
                     ALQFFLANGFEKRNEGLDVFIRPKANNEQ"
     gene            complement(23669..25171)
                     /locus_tag="PK460_01890"
     CDS             complement(23669..25171)
                     /locus_tag="PK460_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53883.1"
                     /translation="MQKRNKFLSFLALFTFLFGVSVTKTQVARSPQKAEAAEFTVTWN
                     VGEQGYTDGEFAGSKVIDDNFEINFYSGRYLLAPRYFSEGSGYISLQGSGDWLGNGFL
                     ISPRPTINNYFINKIVITATDAAYTSKMRTQVGQTMRILFFRTNEFFSGATYTFEELS
                     KFRYFNLENYAGNEFRIKQISLTYTDVPPAAWVNLTSVTKAKDHYDIKRGVHLLIDKP
                     TIEPSNAFYDYFLTTEDTRLRINGPYVVAEDLCENAVVTINTFGRDAGGERLTTTFTV
                     SVTEAPLTIDGAVGMGYGSIDEKLYRFENVRVQDATLDSDNIVLHASATATNKINVMH
                     PGFNPLNTYTYVKGGTMSLFAKVRRNTTTGKNFLVEPEVISYTDKAILFANTIMASDE
                     VGQCVTKYATVRAIINEMTVEELAKLKNAGVVNGINFSPVRARYVAWALHLGESDPYG
                     DGTPISGMPLVPENSTTQNNNVLLVILLSFSVAVFLYFLTSKRKKLYLKK"
     gene            complement(25335..26897)
                     /locus_tag="PK460_01895"
     CDS             complement(25335..26897)
                     /locus_tag="PK460_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE53884.1"
                     /translation="MLNLFKTYKGDNVPKLTKYVYSFTGIGRDAAYTLINLFLMIYLQ
                     LTLPESDPQQYKLMIMTIMITMLVIHLWDAVNDPIIGMLIENSRFKLGKYKPWILIGG
                     VLNGLLLMVLFSFRPFGGWGYVIFFIVTYLLWEISFTLNDIGYWGMVPSLTRDPKQRN
                     QITMLMAIAINIGAFTVGGLVPSLYPGRAIQVFRTLSIIIGSVLIFSQLLLVFLCKER
                     DRSEVDSEEIIKVKDIFKVVFQNKPLLVMLGAIVLHYLGGSMFNNLGQNYMHFTFGYA
                     EGGQAFFYFLVGYAVGSIAANFIFGFLVKKYTRAQILRGAFTISAIGNSLLFVIGSTH
                     AFGWLQIPNSIYQWILPVLALIIFTPNGVFYLTLLVQITNTVEYNEWKFGERKEGVVF
                     AARPFAAKISNAITVIVVFLTLTVGGVYDITNKISDYEVLGGLPEGDARRLITEEVQD
                     LAQAAIDGANLGRARFVLILGMALLPLICQTLAYLLVSRKYPIDEKMYDQMVKEIDER
                     RETAAEEQVVEA"
BASE COUNT         8254 a         5180 c         4497 g         9071 t
ORIGIN      
        1 ctctctactc tcatctttct aagttcaatc ccatattttg tagccatttc aactccccct
       61 ttttaaatag cataacttta ttatacacac cttcacatta gttgtaaata atttttactg
      121 tttttgtgaa caaaataaaa acaatgtgtt taaatttaca tgctgttttt ttaaaatagg
      181 tgtaatttat aactgaaaat agtttatttt gattttcaaa gttttatcta cgcaaaaaaa
      241 cttttcccca atgcgttttt tgattaagtt aaagtcgcaa aattcaaaaa ccatcatttt
      301 ttcaaaattc aaaaaatccg aactttgaaa tcgtttttac cttattcgat atgtaataaa
      361 tgcaataaac ttaagttgct taaaaaaata ttttaatccc tataaaatac ggatttgtta
      421 ctgttgtcaa aagtattgtt gacaatgatt ttaaatttag aattatttaa aaacacttca
      481 taatctttct caattgtaac tatatgaaat tgaacaagaa catccatatc attaaactct
      541 ggtgaataaa catcaaaatg tagtttagtt ttttcttcat tttcagataa ataaacattt
      601 tgaagcgata tgtcgatttc cgtacttgtg tgtgcaaaag gtacgattaa taatacattg
      661 tcaatgaaaa agtcatcatc atctttgatg tcaaacgtca aatcactaag cgttggttga
      721 gcttcttctg catatttagt gattgtcgcg ttcagagtgg taagtgaatc aacaaacaca
      781 atatccatat atccgtaacg tccttcggta gtaacatctg gcgctaaaga agttccatct
      841 attgataaac tttttttgac aataaaggat tgttgaattg actcttcatt ttctttctcg
      901 ctcggagttt cagaattatc atttgttatt gaattatgtc cataaaaaac cgatttatta
      961 ctattatcta aagtgttatg aacaattatt tcaaataaac ctttatttaa tgattcgctc
     1021 gcccatgtac caattactac aacattaaat tgaacgagca cgtccatatc attgaattca
     1081 ggggaaaaaa tgtcgaagtg tatctttgtt ggctcattat ttccggcaat atcaagtcct
     1141 tcaagcgaaa tttctgtttc actactggtg tgtgcaaaag gtactattaa taaaacattg
     1201 tcatcaaaaa aatcatcatt atctttaatc tcaaacgtca aatcattaag tgttggctgt
     1261 gcttcttctg catatttagt aattgtcata ttaagactat tatgagaatc gacaaaaata
     1321 atatccatat atccgtagcg tccctcacca atgacatcag gatctaatga ggttccgtct
     1381 attgataaac ttttcattac ggcaaaatca cgatttgttg gttcacttga tttatcagtc
     1441 ggatcagaag tgtcgctaga agaatttaca ggattattcc aatagtaaac atattttgta
     1501 acaaaaaagg tggataaagc aattgctgtc actgttaata atgaggggat aagcagtttt
     1561 ggaaatcccg gtcgcttgct gcgtggtgct ttgtgagcag cttctaaaac tttcggatct
     1621 aaggtttttg gatactcggg aatatccttt tttatttttt tgagtttatc ttcatattta
     1681 ttcatgttca agttcctcct ttaacatttt gagcgcacgt tcgtgccgct ttcttgctgc
     1741 acttgcgctt atttttaata ctttagcgat ttcactaaaa ctatattcat tcacatagcg
     1801 caagataatc acatctttat atttagtttt aagtttgtta acttcatccc aaatatattc
     1861 gttttcatta agtggctcgt cactaatcgc aacaatcgga tcgctaattg acactggcct
     1921 tttcttgcgg agcatatctt tacttacatt aacaacaatt cttgttaagt aacgtcgttc
     1981 atcatcgctt gatacaaagt ttttatttat tcgtaagagc cgcataaacg attccagcac
     2041 agcatcttct gcatcttcga cactttgtaa gtaagagtag gctatttgga aaaccataac
     2101 tttgtaagtt tcataaacga ttgcaaactg ttcgttagtc atattctcct tacacaatac
     2161 aaacgtaact taaaatcatt ttgtgacatt aagtgtaatt tgaccctttc tattagtaat
     2221 aataattagt tctctataat acataacgat tataaagcgt cttttgtgac aaaaaacttt
     2281 tttttggtaa aaacattctt aaatcaagtt tatacttttt taaaatagat tttgttttaa
     2341 tgagttttag cggcaaactc aacaactcat aaaacatagt aaagttcgga tttttagaac
     2401 atttgtgatt ttcgtattta tcctaattaa ctataaaaac accccttcca cagttaaata
     2461 ttattgacac ttatgtatag gtaaaaaatt aacacttctt taaaaatgag aaaaaaaggc
     2521 acgaaaagtg cttttttgat agtggggcga agtctgacag tggcggactt tgtcaaactt
     2581 ttagcttatt tggtaattta ggagcgtacg gcaagagttc atctaacggt tgtttgtcga
     2641 gattttctaa aagataaata aggtattttt ctatattcaa atggttaatt aatgcagtgc
     2701 gaataatgga aaagataatt gttgtgtact tagcgcctgc gtatgatcca gcggtcataa
     2761 agactttgcg attaatgaca aatggtttta cggcgcgttc agcaaggtta ttcgaaattt
     2821 caagatacgg ataatcaaga tacgtcaaaa gttcatccca aattttccga acataatgga
     2881 cagcaccttc aagggcagag ttcggcgaga gtggagtctc aaaaacatag gttttaagtt
     2941 cattaagcag cggaatttgg tcatgatggc gttttgcaca tatttcggaa ggaagaacag
     3001 cgtccgtttt atatttagct tcgtaagcga aaagttcgtt aatgagcgtc agtattttgt
     3061 aacttaatgt tgttttaact tcggtcgtgg ggagtgcttt taagatgtca ctgaagcgac
     3121 gacgcgcgtg agcccagcag cgttgtaatt taatattttg gttatttttt gcaagtttgg
     3181 tgtaaccagc ataatcatca cagacgatat agccgtcata cttagaaagc cacttagcgg
     3241 ttaaatcaat actgcgtgat tcatgaaaat cataaagatg aatttgttgg tcaaaataac
     3301 ttgaagcata gacataaaca tagcttttct tgcgatttaa ggcgttttcc gggcgtttag
     3361 cgatacttaa tgtcgtttca tccgcgtgaa tgactttaac cgagttactg agtaaatcgc
     3421 gtttcatggc gttatagacc ggtgttaact tcgtcgcgag tgtttgcatc caggtggcca
     3481 taagttgctt cgagatgggc acgttaagca cattggtaag atgtttttcc aaatgatgaa
     3541 aaggaatccc aagttcgtat ttgtggtaag caatataggc agcaaaagag ggggttaaca
     3601 cggagccagg gaaaacttct tgactaagcg gataaacgaa tgtttgatcg tgtttacatt
     3661 tgtaggaacg tttaattact ttcgtcactt taatcgtgga aggaattact tcaatgatgt
     3721 aacgttcttt ttggctagca agcgttaagt catttgaaca ggcgggacaa acaagcgaat
     3781 ccgggtcttc gtaaactgta tcaatgacag cggcttctaa atcaacatta gcgaaattct
     3841 tcccaccttt tttgcgacca acccgcgctt ttgtgtgttt gttttcttgt aaaatatgtt
     3901 ctggttcatt aatgacgatt tcgttaatct tttcactttt agcgacgaac ggtttcgtgc
     3961 gttcaatgat aagtttttcg cgaacaattt ctttgtcttt gagtagttca ttaagttttt
     4021 gttgttgctt aaaaatgagg agacgttgtt cttcgttttc aagttggagg gcttcatttt
     4081 ctttttcaag aatagcgatg cgtttaagtg cgtcttctaa attcataact atattttagc
     4141 acatataaaa aaagttagtt atatataata taactaaaat ttttataaag cgtgatttaa
     4201 agcgaaactt gcttgcggcg ttcgcttatg agcgcgacac ctttaagtaa ataagcaaga
     4261 tcgtctttcg tgatggtgaa cgactcttca agcgcgggga cattaaaacg agcgtaagaa
     4321 agtttattaa taaagagcca ttttccgtat tcatcttcgt aataaatttt aaccattttg
     4381 cgacttttac taacaaaaat aaacacggag cggtgaattt cgagcggaga aaaagaaagc
     4441 gaaagaatgg actccatttt aataatcccc atccgcatat cgattttatt cgtgtagaga
     4501 aaaatattgc gaactttatt aagatcaatc ataatatttt ctccaaaata gctagtgctt
     4561 gttgtagcga agttgtcact ttcacctgcg cgacttcaat gacaacaaca gaatgatcaa
     4621 accgcgtgaa gttcttcgac ttagcacgta tttcttccgg cttaagcaga ctaacattaa
     4681 catcgtcatt actaagcaag acactttttc ttgaactatc aagtaccttg tttaaacgaa
     4741 cgaaagaacc atcaatatta tcgtaggcat tgatccaatc gcgtaatgtc gtgcgattaa
     4801 tgcccttttg gcgagcgaac tctgagaccg acagcttact catcttaaat tctttgactg
     4861 tctctagtcg ttcataatca gttttatatt tacccataaa aaactcctac ctttctaagt
     4921 aagagtttat gtaattcatg ttagtttgaa aatggtgtta tttttttacc tatacacact
     4981 tattgttcat ctacgccaaa tctacgccaa atctacgcca ggtaaagaaa aaaggcccat
     5041 ataatcggcc tttttcgtga tatggtgccc cctgccagtt tatcgccgtt cattttacca
     5101 catcttacca cgtcttatca catcttattt tggcttaaaa acgcgttttt taaaacctgt
     5161 tttcttacca catcttgcca catcttacgg gattttactt tttagatttt tatctacgcc
     5221 aaaaacacgc ctttctttta tataaatata ttaaggttat tccaataaac aaaaaatagt
     5281 ggagtacgca aaattagaac aatttatccc gctagtccgt aatagtacgt gttgtaaaga
     5341 gttactgcaa actctggtgt taacctatgt aaatcattag tgttttcttc atcaaaataa
     5401 aaaccgtaga atatgtaata tccatacatt gtttcgggtg ggatatagtt taaaacactt
     5461 cgactttgta aatcatcaat ttcttcaagc gttagtttta tattatcttc aaaacttaaa
     5521 gttgttattg ttaagtcttc ttcgccaaat acgggaatta aataatcttc aaagttctgc
     5581 caatcaacaa tcttgcgctg aataaacgtt atatcaatat agttcggttc cacaatcatc
     5641 ccatctttta tgacgaacgt tatcgttacg tcataagttg gcatgataaa gtcataaaca
     5701 aagacacttt gaccaacatt attttccgcg gttgttgcat tattataaaa ttcgtcattt
     5761 acatacatgc taacaataac gtcagttaac ataactgtct cgatccgtat tgcttctcca
     5821 gcggaataac gatctttatg ttttgataaa actgttcgat ccgtgtcaat aattgtaaaa
     5881 cgatacttgc gagtattttc gcaaccagct agtaataata atcctaataa gaaactcgtt
     5941 aacaactttt tcattcaaca tgtcaccttg tttttactta aacgctttct acttcataca
     6001 ctaccaagcg atactagacg caacttatat taagaaatat cattggcgaa cctccgcctt
     6061 gcgggagcaa taagccaagt aattgctcga tagcatgacc aacgagcgaa taaagcggaa
     6121 atttacttgg gccagcagca taataaagtg tattcgcctc atcaatacaa tattcgatat
     6181 cgggattttc taaataaaac acatcatagt cacgttcatt aacgaaataa gcaattagca
     6241 aatctagttg aatgtcttgc cattcgaaaa aaccactggt gatcattttg tatttttgat
     6301 ttctaaaagt aagttgttgg gggatgttcg ctaaaagcat tgtgtcatct gtaaaattat
     6361 tgttagtgtc aaataagttt gcgaatgtgt caccacttat aacctcataa aaccgctcta
     6421 tttgttcccc aacattaagg ccaaaagtaa ctgcgtcata cacagaaata atcccgccta
     6481 ctgtttcaat cgtgaagttt acattatttc cactaagctc cgttagttct ttaaattcta
     6541 gtgaaggcaa ggaaagcgaa aaatccgtta tttcttttcc ctcgctattg aaaacttccg
     6601 taatcgttgt ggaaccgcta aaactatacg agctgtatgt agtgacaata tttggtaatt
     6661 cttctagttc ataatacaca tcatctttca caacgaacat atttgtgaca tcgaacgtaa
     6721 actcgagagc ttcattctta aaagcgtacg tattaattgt cgggcacgca acatttttac
     6781 tttcaggaag ttttgcgtaa cgacgcactt tagttttccc tagttcatta acaaagttcg
     6841 ttacggcggc tacgtcatcg ttatagtaat gattaataag cgctaaaggt gcaagactat
     6901 tacaactaat ggtatgttta acttcagcaa attcctctgt tttaagcatt gggaaaactt
     6961 cactaagtgt ttggtgatta gccgctctgc atgatgttaa tatcaagagt aagccggcga
     7021 ctaaaatttg cgttcgtttc attttccctc cattaattac tataaaaaac tgatttatta
     7081 ctattgtcaa acgtattatg tacacttatt ttaaacgcac tttcacttaa tagatcactt
     7141 gcccattcac ctatcgtgac aagatgaaac gtcacaaggg tatccatatc attaaattcc
     7201 ggtgaatata tatcgaaacg cagttttgtt ggttgctcct tactcccaaa ttcgatttgt
     7261 gtaagttcaa taccttcttc tgtacttgta tgggcaaacg ggacaattat caagacgctt
     7321 tcactaaaga attccgcgtt atttctaatt acactcgtta atgtactaag tactgggtgt
     7381 gcgtcatcag catattgatt aattgttgca tctagcgcaa gctgtgaatc aatataaacg
     7441 atatcaaaat agcctagttt cccttcaaca agcatttctg gggcaaggga cgcacccggt
     7501 attgctgcgc ttgccgaaac ataataatca taaacttccc cttcttctgc actggtatta
     7561 gatggttcac ttggcattcc tgaattactt ggggtatttg aaggcgtgtt ccagccataa
     7621 acatactttg tgacaaagaa agttgataac acgactgctg ttacggttag caaggaggga
     7681 ataagtagtt taggaaagcc gggtcgcttg tagcgtggtg cttgttcagc ggccctgaaa
     7741 actttttggt ctaaaccatc gggatactcg gggatatcat ttttaagctt ttttagtttc
     7801 tcttcatggt tattcatgac taagctcctc cttcaacatt ttaagcgctc gttcatgacg
     7861 ctttcttgcg gcacttgcac ttatttttag cacactcgca atttcattga aactatgttc
     7921 attgatgtaa cgtaagataa tcacatcttt atatttatta ggaagtttgt taacttcgtc
     7981 ccaaatataa ttgttttcat tttgccctgc gtcattaact gcaatcacgg gatcactaat
     8041 tggcactggt cttttcttgc gtaacatgtc tttacttacg ttaacaacga ttcttgttaa
     8101 gtagcgtcgt tcgtcgtcac ctgatgcaaa ttttcggttt attcgtaaga ggcgcataaa
     8161 tgactcaagc gttgcgtctt ccgcatcttc tacatttcgt aagtaagagt aagcaatttg
     8221 gaaaaccatc actttgtaag tttcataaac gatcgcaaaa tgttcgttag tcattttctt
     8281 cttacataat acaaacgtaa cttaacgtca ttttgtgaca ttaagtacaa tttgctcctc
     8341 tctattagta ctaattattt ctctactata atacataacg attacaaagc gtgttttgtg
     8401 acaaaaactt tttttgttaa aaactcttaa aaaaatcctt atcttaagtt tacacttttt
     8461 ccaataaata ttatttttaa gtgtgattta tagctagaaa aaaattgatt tttaaagttt
     8521 tatcgatgcg aaacttaaaa taactaagat tttgtaaaat actactctcg ttatgttaac
     8581 gaaccttcgt tttaaagaaa tattgtcata tatactggta aataaataac gccttcgtgg
     8641 actcgtaagt ctttagtatg taacaaatac gattggccta atttattacc gaattttact
     8701 ttcattttct ttaaagacga ggttgtataa tttttacctg attttacttc aacaagtgat
     8761 attttccgtg ggttatgtgg atcactaatc ataaaatcaa tttccattgt atttttagca
     8821 ttcttccgat cattgcgcga ataaaaataa agccgtttat tactggccac gagcatttgg
     8881 gcaacgatgt tttccaaaaa catcccagaa ttgatattta attttcctgc aacaatactt
     8941 tgggctactt ctgtttgcat aatctcattt tcattaaaag catggctgaa aagcaagccg
     9001 gtatcgccca tatacatctt gagtgttaag gattcacgat aaagcccaag tcctacggtc
     9061 ggatcacttg cgttaaagca aggatttgtc atcatcgctt cgttaagcca aacaaaggct
     9121 tcttcataat cacgaaaacg ggcatcttta ctaagagcac tgagtgtgaa ttttttttca
     9181 tgttttgtta actgattggg gatttcatca aaaatactaa taactttata ttcattgttt
     9241 ttcgcatatt tcctaatatc attccgataa agttgcaaga tatctctttt tgcccgatca
     9301 acacgagtat aatcattcgt ttctaaaaac attgctactg cttgcggcat accaccgata
     9361 agcatatatt ggcgaaacaa tttcatcgca agttgatgta gttctttggc cggtttccgc
     9421 tcttcaaatc gtttttgtaa aaaaggaata agatcattat gccccgttgc ccaacaaaat
     9481 tcttcaaaat caagtggata cattttaatt gtacgttctt cagaaggtag taatatctcc
     9541 gcaacattct gtttgagtga taataaagat ccagtttgaa tataatcata gcggttatct
     9601 tttactaaat gtttgattag ttgacgggca gccggaaaaa gttgaacttc gtcaaatata
     9661 attaacgttt ctctttcgaa aagtttttga ccagttaacg aagataagaa aaagaaaaaa
     9721 tcatttaaac tactagcata gttagtgaat aaagatttaa catcatcgct agcaatgcta
     9781 aagtcgatta gtacataact tttatattgt tgttttgcaa actcttcaac aatcgtgctt
     9841 ttgccaacgc gcctagcccc ttcaattaaa agtgctgttt tgccaccttc acttttccag
     9901 ttaagtaagt cactatatat ttttctttta aaaatcattg tttttacctc tctaacacca
     9961 ttttacacga aaacaaagtt tttgcaatcc ttattttaca cgaaaacaaa gtttttatag
    10021 tgcgattaat acacgtaatc aatttttttg ccgtttattt tattaaaaca ccaattctta
    10081 atcatttaag ttggctttct ttgttatttt cgtttatttg ctacgtttgt tacacttttg
    10141 gtgacatact tcacaacgtt ttattaaaaa attataattt catttaaatc agcagctgat
    10201 ttctctttca taattcgaac aatttcattt gttctttttg gggattttga cacaatgcta
    10261 tataactcat aaaacatact aaagttcgga attttagaac ctttatcttt ttataattgc
    10321 tgtaatttaa gacaaatata cgtttataga tggcgaaaat aattgacact tattgttcat
    10381 ctacgccaaa tctacgccaa atctacgcca ggcaaagaaa aaaggcccat ataatcggcc
    10441 tttttcgtga tgtggcttta taaagttgga gagattctta atgaacctac gagattatta
    10501 tatacttaac aaataattat ttctacactc tataggcgta agttttgttt tcattattat
    10561 tcttgtctta ttgtagtaat ttatatattc atgaatcgtt gttattaaag cttctacact
    10621 atcataatca cgcttgttat tgtcccaaat ttcttgttta agccgcccga agaagttttc
    10681 tgttggagag ttgtcaaggc aatttccttt tcgactcatt gattgtatta tccccatgtt
    10741 tcgaagttca acaatatacc ttgagttttg atattgaacg ccttgatcag attgaagtat
    10801 catccccata agattattcc cgtgcttctt ttggagttct cttaacattc ggatgattcg
    10861 atataccttc gcatcagttc ccacagaata cgctagaact tcacgggtat gaaaatcaat
    10921 cacaggtgac aaatatactg attcaccaaa cattggaaac tctgtcacat ccgtcccgcc
    10981 tttttcatat ggccttgttg ttttaaaatc ttgattcata atgttttcaa tttttaaacc
    11041 aaggtttcct tcgtacgaat tatactttcg ccacttaatg actttgggtt tactaaaacc
    11101 tttcgctcgc atgatttcta aaactttgtt tttaccgaca atccacccgt ttttcttcaa
    11161 attattgtat atttgatgat atccgcaccg tttcttatga actttaatga ataatcgctc
    11221 tatttctttc tctaactcaa catacttatt ttgccttgtt ttattcgcgt ttaatgtgta
    11281 ataaaagacg cttgacgata aaccagacag ctctagaagg atacttaatt tgtatttagt
    11341 ccttaactca tcgacgattc ttactctttc ttgaaccgtc gctccttcta gtttttgagc
    11401 taaggactgc gattttttta agtacgctat ctccgcacgc aaccgctcgt tctctgcttc
    11461 aatctttcga tcaacaaccg ctttaaaacg gtcttccttt aataagtaat tcgctcgcgc
    11521 tttcgtatct tgaatcgctt cttcgccgcc ttcacggtat ttcttcaacc aatcacttaa
    11581 tactcccggg ttcgctaaac cgtgctcaca cgcaacgtct cttatcgttc ttcccgctaa
    11641 cacctgtcca attgctaata actttgtatc acgcttatac attcgccgca cacggctcaa
    11701 aaatggctcc tcaccatgca tcttatacaa atttattatg tacttgatgt tcgttttatc
    11761 atatccccca taaagctcgg aaatatgaga aatcgaaaga ccttcttcga tatgcatttt
    11821 acatatcttg accttctctt tcatcgttat ccgcataaaa aatagctccc ttcttgggta
    11881 ggtaccttca ttatacctct ctccaagatt tggaagctaa ttcatttttc aatggtgccc
    11941 cctgccagaa tcgaactagc gactgatcct taccatggat ctgttatgcc atttaactaa
    12001 gggggcgtcg aattgaatta tagtacataa cgctaaatat tacaataaat gtaatacatt
    12061 taatgaatga atagcctctt tttgcataaa gaaaacggcg agaatgactc accgtttaat
    12121 tagtgcatta attatttcta ataaatatcc gcacaaagtt tgcgggtagc gcgattagtg
    12181 ttaccccaaa tatagcacga taaattacga ttatcgataa atgggttacg aatcttttga
    12241 tattgttcaa tccctttttg ccgttgaccg tcgcgttcaa tagtcggagc ccattcttgg
    12301 tcccagcgaa tcatatcggc aatttgaaag ttttcactaa tgctacaacg atctgggtac
    12361 attagtacca tatataattg cgctctagca atttcaccac gcacaattgg cgttggtgag
    12421 tagccgtagt gcacaatcgc gtccacgagc tgtccagcgg tattgcgtga taagtatggg
    12481 aaccaacgct ctggatcttc ggcgtaattc cattcaatat aagagtgatt gctcttatgt
    12541 tcatttaaag cagcatcaca ggcgaataaa ttagcgatgt cacttgaaat atttttagta
    12601 ttaccactag cgcgtaaact tttatcacca tctgctagtt tggattgcgg gaaaacgtgc
    12661 tcacgattcc atttaccggg gtacccagcg tgcggatgac tacgtaagtc atacagtgat
    12721 aatacattac ctgttttatt tggatcagcg tccattactt ttattgcctc taaagcatca
    12781 tcgtatgtaa ctctattatc taaaacacct ggcacataaa acttcgacca catgtaatcg
    12841 gcaagtgctt gacgaagctc ttcaccgcgg agtgtaaagt caatcgtgtt ccaaaaatta
    12901 ccagtgtaag caggtagtgg tctaaaattc caattttcag ctggttttaa tactggttca
    12961 ggttcaaccc attcagaaga cacctcttca cttgattctt caacactcgt aacttcttca
    13021 gttgtagatg tttcactgac gatacttggt gtatcactaa tgtcactaac gcttggaatt
    13081 tcactctcag gtacttcaat actctgtgat gtaggtggtt cacacgacgc taaaagcgta
    13141 actccgatca gcgctaataa taatcgacgt attttcataa ttaaaagacc tcttttctta
    13201 tgtgatttaa ttataacaaa gaggcctttt tttgtaaatt aaagttaggc aaagggcgaa
    13261 atactaactt cgttatttgt taccgttaag cgataagtaa tactgctatc gatattgccc
    13321 tgaacaactt gttcagcgat aaatgtttct aaactctttt ggataaagcg ttttaatggt
    13381 cgcgccccaa attctggagc gtaagcatta tcaagcacaa agtcaacaat cttttcgtca
    13441 tatattaact tgaagttatt tttggcaaca tgagccatta gttcatcaag catcttgcgc
    13501 gtgatttgtc gttgtgtgtc tttactaagc ggtaagaaat acacaatttc atcaatccga
    13561 tttaagaatt cgggtttaaa agtcgctttc actaagtcat caatctttga tgtttgattt
    13621 ttaaggaaga attcactacc taagttactc gtcataataa tgacagtatt tttaaagttt
    13681 acaacccttc cttgagaatc agttaagcgg ccatcatcta gtagttgtaa gagtaaatta
    13741 aagacttcag gatgcgcctt ttcgatttca tcaaataata taattgaata aggactacgc
    13801 cgcactgctt cggttaattg cccgccctca tcataaccaa cgtatcccgg tggggcacca
    13861 ataagacgcg agacactaaa tttttccata tattcactca tatcaatccg gacaatatgg
    13921 cgttcgtcgt caaaaagagc gtaggctaag cttttggcta cttcggtctt accaacccct
    13981 gttgggccta ggaataagaa actaccaatt gggcgatttg ggttattaag tcctgcgcgt
    14041 tgccgtaaaa tggcatcact aacaagccgc agggctacat cttgccctaa tacgcgtttt
    14101 tttagttcat cataaagcga taaaatcttt tctttttggc tttccataat tcgttttaac
    14161 ggaatccctg ttgcgcgtga gacgaccgca gcaatttcat tttccgttac tgtttctttt
    14221 aatactttat tatctttagt ttcttttgtt aaagtttcga tctttgcttc aagttcagga
    14281 attactttat attgtaatgt tgaagcttct tcgtaattac cgctattaaa ggccacactt
    14341 aagttaaagc gtgcttgctc taattctttt ttagccttac caagcacatt aagttcatct
    14401 aattcatgtt gccattcttt actaaaggct tcatcttctt ttttaagatt ttccagttct
    14461 tcatttactt tttgtaaacg aactttactt aagtcatcat tttctttact aagtgcaact
    14521 cgttcaattt caagttgccg catcttccgg cgaatttcat cgagttcggc gggtaaactt
    14581 tcaacttcaa cacgaattaa tgcacaagct tcgtccacta agtcaagcgc cttgtcgggt
    14641 aaaaagcgtt cggtaatata acggtcacta agtactgctg cactcacaag tgcattatcg
    14701 cttatcttaa cgccgtgatg ggcttcgtaa cgcgctttta gtccgcgtaa tatcgaaatt
    14761 gtatcttcaa gcgtcggttc ttggacaata agcggttgga atcttctttc aagcgctcgg
    14821 tctttttcaa tgtaagtgcg atattcgtca agtgtcgtcg ccccaatcgt atgaatttca
    14881 ccgcgcgcta acattggttt aagcatatta ccagcgtcaa tggctccttc ggctttacca
    14941 gcgccgacga ttaaatgaat ctcatcaata aataaaatga tttgatgatt actttcttta
    15001 attttcttta aaatggcttt gagccgctct tcaaattcac cgcggaactt cgcaccagcc
    15061 actagtgcgc ccatatctaa ttcatagatt cttttatctg ctaaattctt tggcacatca
    15121 ccagcaacga ttcgttgcgc tagtccttca ataatagcgg tcttaccgac ccctggttca
    15181 ccaagtaaaa taacattatt tttttgcttt ctcgctaaaa tttcaataat gcgtctaatt
    15241 tcactatcac gcccaattac tgggtcactt ttatttagtt tagcgtcttc atctaaaaag
    15301 cgtccaaact tctctaagat tttttcatct tgttcataat ttggaaaagg attaaaattc
    15361 ataatataac acctccttag cactttaaat gtctgagtgc taagtctatt atatactaaa
    15421 attggcactt gtcaacaccg agtgccattt tttgtaatat ttttaatatt attagtctaa
    15481 attaagttta cttgctttta aaatgcgcat tatcttatca tcatttggac ggaaaataac
    15541 atactgtgtc gcataacgaa tagcatcaac ataattacct tttgtataat gattaataat
    15601 tagtggtcta atcgtatcaa atgctatttc actaggatac ggtttaaatt ttgaatacgc
    15661 aaaaagtttt ttaatttgtg cccggcttaa ttcgtatggg gcatcttttt tcaaggcagt
    15721 taatacactt gtaatttctt cattaaaacg tgctggtgtt tcaccatctt gatacacgat
    15781 tgcttggtaa taaaaggcat aagcttcggc gaacttacca tcaagataaa gcgcatatcc
    15841 tgcataacgc aatgctgcac taatgtttgg ggcgctataa gtaatttgca agtaccacat
    15901 tgaaagccgt aataaatctt gagcgcgacc atcatacttt aaaccttcaa ggattttaag
    15961 aattggttta ggattaaacg gattccattg atgagctttt gaatacatca caaatgcttt
    16021 cgtgtaatcg gccattgctt cgtaaatcaa gccttgcgta taataaatac ggtaaaaagg
    16081 gaatgtcacc aaaatatctt caacatttga attagccatt ttcgtgtata acgctttttc
    16141 gtattcaagc gtaaagttat aaagtggttt gcgtgtcgct ttatcatcaa tcttccgcgc
    16201 atgtgcgtcg gtgtacttaa gcaaagcatt accaatttca ttacgccgtg atagcgcaat
    16261 tttttcttca ctcattaaat ataaacatac agggacaagt acttcagggt taacacgaat
    16321 ttcattaaaa atcgtttttg tgtctttacc taaaaaagtc gccgctaata ctaaaatctc
    16381 cgcttcagtt ttatctttgg tgaagttttc aataatataa ttaaaaatat tatgacgatc
    16441 aagatcgcta tcaccagtta agttaatccc taagttaaat ttactgtttg ttcccataac
    16501 tcaatgttaa caaaaaagtg gcattgtcgc cacttttaac actttaattc gtgcaattct
    16561 ttattaaagt tgtaaaaact taataaaggc gtcttgtcca gcttcatcat ttttaaagca
    16621 tgcggtattt tgtaaaatgt tttcacagac attagtcata taatcacgcg catacttttc
    16681 aaattcagtt ggtttaattt tactgagcat taattcttta ataaaagtag cgtgtttagc
    16741 aatacttggg tgttcactaa tcatttcggc accagtaaca ccttttttat ataattcttc
    16801 aattaaacca aattcattct ttagtcgcgc tggtaaaata aaggttccca tttgttcaat
    16861 taggccaatc ccttcttttt taatgttatg gtattcggga tgagcgtgga atataccggt
    16921 tggatacttc tcatcagtgc gattattacg taaaattaag tacataatgt atttatgttt
    16981 ttctttaaca acaattggtg tgaccgcggc gtggcgttca ccgctagttg cgggaataat
    17041 ccctaattct tcattcgtgt attctaacca attaccagta atttccgcgg cttttgctaa
    17101 aagaaggctc tttgttcttg attctaattt aatgacatta ctatcccatt caagaatgct
    17161 aatcttaagt ttacggtcat gtttatgttt taagacaaac cgatcttttg cactaaacat
    17221 tggcatcact ttcgcgccac cttggaagtg ttcatgatta agaattgaac caccaacaat
    17281 cggaatatca ctattactac caataaataa atccgggaat tgatcgacaa aagccacgag
    17341 ttttgaaaag atacgtgggg taattgtcat cggattatga tctttactaa taacgatgat
    17401 atggtcttta taatacacaa atggtgaaaa ttgtaagtac cattcttctt tatctaaagt
    17461 aagtggcact gctcttaaat tttgccgtgg tggggttgac tcattaccac caaagccaac
    17521 attttctaaa caaagcggac aacttggata cttatcatct tgtttttctt ttaataattt
    17581 tgctgtttct ctattatcta tttctggttt tgctaagtta atcgtcattt caaaatattt
    17641 atcacttaac tctgctctcc agcgaatatt ttccgccact ttagcttctt gaatatagtt
    17701 attagcgata cttaaatcat ataaatactt aattgcttta ttactatctt tcttatgaat
    17761 ttccgcaaag cgttgactaa caacactcgg gattggtgtc atcatcccta acacatcaat
    17821 aattacttct tcagggtctg taaaacttaa atgtgctggt aatccttttg tttcaattaa
    17881 atgttgcatt tcatcaatta acatttgtgg gttattcata ctttgaattt cactaacatt
    17941 aatgacacct ttatatggtt tttttagacc aaatttattt aagagtacat tgcggtaata
    18001 attgatatct tcgctattaa gagcgagttt aagttccgca tacgctaaga gtttttccac
    18061 taaaatttct accatagtaa atacctcgtt tccttcctat tatcgcaaat attaatcttt
    18121 tttggtaatg aagtataaga cgctcgtaaa gtcacgcgtc attcttgttg tcgatgagaa
    18181 cccacttgcc gtccattcag gataaagcgt tagtgcccca gcgcgcaatg cttttccgct
    18241 tacagtgtag cgttctatta cttcgacttt cactaattct tctgcactac ttcgtttatc
    18301 aaggtacttg ataactgccc cgtcatgctt aataaacttt ggtaatacca tattaagcag
    18361 tgaaccaaaa gttgagatat ctgtactttg cggcaaggca aatacttcgt aagttaagtc
    18421 ttgcttcacg ggaatagacg gaaactttaa aacttccggc atcggtgaat gaatgccgct
    18481 actaattaag aggagtgcat tatcgctagc ttcacttagt agtgcgtacg cctttttatc
    18541 tttagcgcta ctaagtgtta actcgtaata cttcccgtcc ataataacac tgcggaatgc
    18601 tttagcaatt tcgttatatg ctttaatttc ttttttgtct tttggcgtta aattattcac
    18661 atctaattca tagccaaaac caccagacat aaaggcatta ttaaagcgtg tttgcagtgg
    18721 taaatcacgt agtaactgat gattaggggc agcactaaca tgcccagaca tgacactaag
    18781 cggataagct ttcgcgagat tggtatgcat tctaatccgt tcataagcat cactacagtc
    18841 actcacccaa atttgatcaa agaacgataa aattcctaaa tcaaaacgat taccgccact
    18901 tgcacaacct tcccataaaa tcgttggaaa ttctttggtt aaccgctgca taatcgcata
    18961 taagccaagc gtataatcat aaaagaatgt gccatattca cccgatggtg aatagtaatc
    19021 acttaagtta cgattataat cccacttaac atatttgata tcggcactct ttaatacttt
    19081 actcacattt tcgacaatat agtcttggac ttctttgagc gttaaattaa gggaatactg
    19141 atggcgtcca gtactaggtt taacactagg aattttaaca acccattcag ggtgttcggt
    19201 aaatatcggg tgatcagcac taaccatttc tggttcaaac cataaaccaa aggacattcc
    19261 taatttcgtt gcgtagtcac ttaggccttt taagccgcgt ggtaacttct tcttatttac
    19321 ttcgtaatta cctaatccgc caaagtcatc atcacgattg ataaaccatc cgtcatctaa
    19381 aaccattagt tcaatcccta atttacttgc ttcacgcatt agttttttaa tttttgcttc
    19441 atcaaatgta agataagttg cttcccagtt attaagtaag attggacgcg gtttccgcgc
    19501 atattcataa cgcacgacat ggtctataac aaagcgatgg aagttttgac taaggccatt
    19561 ttcgccttta tcactaaatg taagggcggc aaacggggta ataaaaggct ttccatcaag
    19621 ttctttacta aatgtttcat catttatgcc gtgctgaagc ctgaccctat tatcgaaagt
    19681 taattctacc gaagctttaa atggtccgct ataaattaaa ttaacaccat aacctatccc
    19741 tttatgtaaa tcagttttct cgttcaaaag taaaataaac ggattatgac gattgctact
    19801 tgggccaaca cttgtcgcca cttcaaagag accaggcact aacttattac gcgaagcatg
    19861 gacttcattt gcccatgtgc cataagttga aaacacatca aaatgatcat tatttaaatc
    19921 caagtttaaa cttgctgctt ttgttaattt accttttccg ctaaggacaa caagacgccg
    19981 agcgatcaca ttagcgcgct taaaaattaa gtaatctaat tgtactttta ctttactaat
    20041 gttgtcttca aggatgacat aaaagtattc atcatagcca tgcggaattg gtaaatcatt
    20101 tactaaaagt acttctcttc cggcaaaacc gtgctttttt acgacaaaat cactcgcaaa
    20161 tcctagttca tgttcaagta aaatacttgg tcgccggtaa tcacctttgc caatcgtccc
    20221 taattctaaa ttaacaaaat ctaaggaata agtgtcatgc ggttctccgc tataaacagt
    20281 tgtcgtccct tgcatattag tttgtttatt aactaaaatg cttaaatctt catttagcgc
    20341 taactttgcg ccatagtaaa ggttttctaa atggccatat ttattaatgg caagcacata
    20401 tgatgtgtca tttgttttaa ggtgtatata tttgtattcc atagattaat tttaacatac
    20461 atacgctatt tttaatattt actaagtaat tatcatttaa tgttaaaata tgtatgtatg
    20521 aaacacgcac tgaactataa ttggtcattc atcccaaaat ttaatgatga atatattaag
    20581 tctatcccgc aaggggtaat aagtgttgac ctcccgcata atatggtcga ctttcctttt
    20641 aattactttg atgaaaagga ttttgatagt gttggtactt acttccgtga aatcgttttt
    20701 accaaggtac ttgaaagtga taaatttgtc ttacgcttcg gtggcgtaaa taattctttt
    20761 acattatatg taaattcgca attggtcggt acatttacta gaccatatat ggaagaaaat
    20821 attgatatta cgaaatatgt tgtcgagggc aataatttaa ttgttgtatg tgttaacggc
    20881 agtgaaaaag ttaacgctcc accatttggt aataccgttg actacttccc ctttagcgga
    20941 atttaccgtg aagtaacact ttttgtatat aacgcattag atattattga tgtaaaagtt
    21001 accgctaatc atttagggca aattgaagta aaccccgata ttagaaacat taataaggtt
    21061 gattactctg taaattatga agtatacgaa ggcacaaagc ttatcgctag tagtatcgag
    21121 cataatttca cagtcgaaaa ccccaaactt tggagtgtta ataatcctaa tttatacaat
    21181 ttagttacta ctattaccgc taataaagat gttaaacaat atgttagtgt ctttggtttt
    21241 cgcagtgccg tatttcttag tgacggcttc tttcttaacg gtgaatacac gcctttagtt
    21301 ggacttaatc gtcatgaaac ttacccatat gttggcgcag cagccacgag ttttctacaa
    21361 ttttatgatg ttgtaaaact taaaaacctt ggtgttaatt atgtgcgttg ctcgcactac
    21421 ccaccaagta gagcttttct tgatgcctgt gattttttag gacttatggt cataaatgaa
    21481 gttccaggat ggcagtatat aggggacgaa gcgtggcgaa agattcattt acaaaacatt
    21541 gaaacgatga ttaatcgtga ctttaatcgt ccaagtatta tcgcgtggtc cattaggatt
    21601 aatgaaagcg ctgaccaccc attctacgaa gagtgtcata aactagcaaa agcacttgac
    21661 cccgttcgcg ctacaacagg aacacgtaac tttactaata gcgaactttt tgaagacatc
    21721 tactcctata atgactttag tcatgacggc acgaataagg gattattagc taagcgtaaa
    21781 gtaacaaaaa gtaaaaaacc ttacatcgtt agtgaaaata atgggcacat gtaccccact
    21841 aaaccatttg ataacccgct tattttagaa agtcatacaa aacgccattt agcggtgctt
    21901 gatagtttct ttggtgataa aaatattgtc ggtgtttccc cgtggtgtat gcatgactac
    21961 tacacgcacc acgaatttgg tagtggtgac aaaatttgtt atcacggtgt gttagatata
    22021 tatcgtgaac ctaaacccgc ggcgtatgtg tatacttcta accttacaag tgaccctgtt
    22081 ttattcccgc tttttcgcgg tgataacggt gacttaccag aaagtaaatt atataaaacg
    22141 atggtgctaa gtaacgcaag ttacattgat ttatacaaag acgatgaagt tgtaaagcgc
    22201 tactacccgc ggcgtgactt atataaacat ttaccgcatc caccatttat catcgatact
    22261 tttattagtg ataactttaa gtcaacaatt aaattttcaa agcgtgatcg taaaaagatc
    22321 gcaaaactat taaattatgc cgctctcaat aattttagtc ggctctcact ctggcaaaag
    22381 ttacaactcg ggtggttaat tgttaagtat cgccttaaat gggataatct tgtcgaatta
    22441 tggttcctta atgttgtcgg ctggggtgat aagtcaagcg gctacacact agtgggctat
    22501 aacgatgaag atatcgaaat aattagaaaa agaatcgaaa acaccactac tacgacttat
    22561 gaaatcgaac ttttaaaaaa tgaattagtg aacacagata catttgatac aacaattgtt
    22621 acaatccgta gtctaagtcc ttatcgtaat cattatgact ttagtcctgt ttcagttgtc
    22681 gttactggtc ccctaaaggt ttacggacca gaaatccaaa acttacacgc tggtgtactt
    22741 aatgtgtatg tccgctccat taacacgacg gggcgtggta caataactat tggtgttggc
    22801 ggtacgatta aaaccttcaa cgtcgaaatt aaatagaaag gagaagcagt aatggccgaa
    22861 aaaaatcttg cacttttaat tgatgccgag aatatatcac ctgattatat gtcaattatt
    22921 cgtgacgaag ctaataaata tggcaatcta acttataagc gcatttatgg cgattgggca
    22981 agtagcaatc tgaccaaatg gcgcagtaaa tgtattgaat ataacttaga gcaaattcaa
    23041 caagtctctt acgtcagtgg taaaagcact agtgatttta cactcgtaat tgatgcaatg
    23101 gacattttat atagtgacca agttgatggc tttgtccttg tcagtagtga tagtgacttt
    23161 acaagacttg ttagtcgctt acgccaaaac aataagttta ttattgggat gggtgaaagc
    23221 aagaccccct tatcacttgt aaattccgtc gacttattta tatatctcga taaaattaaa
    23281 aacattcgcg acaaaaacgt taaaccgaaa aaacgtggcc gtcccgctgc tgataagaaa
    23341 gaggaagaaa gcatcattag tcttgatgac ttaatcgatg ttttaacgaa cattatcagt
    23401 gaaacaagtg atgaaaatgg ttgggcatac tggagcaata caaacaatac gcttgttcgt
    23461 aagtaccccg gttttgaccc tcgtaactac ggctttaaag gtaaagcttt acaatttttc
    23521 ttagcaaacg gctttgaaaa acgcaatgaa ggcttagatg tctttattcg ccctaaagca
    23581 aataacgaac agtaattatt atttgtaaaa gaaaagctca gtgatacgta atcaccgagt
    23641 ttttttgtta gtaaactaca aataattcct attttttaag ataaagtttc tttcgttttg
    23701 atgttaagaa atatagaaac accgcaacac tgaaagaaag taaaataact aaaaggacat
    23761 tgttattttg tgtagttgaa ttttctggca ctaaaggcat tcctgaaatc ggggtgccat
    23821 cgccataagg atcgctttca cctaaatgta atgcccatgc gacgtaacgc gctctaacag
    23881 gactaaaatt aattccgtta actacacctg cattttttaa ttttgccagt tcttcgactg
    23941 tcatttcatt aataattgcc cgcactgtgg cgtattttgt tacacattga ccgacttcat
    24001 cacttgccat tattgtattt gcaaacaaaa tagccttgtc tgtgtaacta ataacttctg
    24061 gttccactaa gaagttttta cctgtcgttg tatttctccg cactttcgca aagagactca
    24121 tcgtcccgcc ttttacatag gtataagtgt taagtgggtt aaagccaggg tgcatgacat
    24181 ttattttatt cgttgcggta gcactagcgt gtaaaacgat attatcgcta tcaagagtag
    24241 catcttggac gcgaacattt tcaaagcgat agagcttttc atcaatactt ccgtaaccca
    24301 tgccaaccgc accatcaata gtcagtggtg cttcagttac actaacagtg aatgttgttg
    24361 ttaggcgctc accacctgca tcgcgcccaa aagtattaat tgtcacgacc gcattttcac
    24421 aaaggtcttc ggcgacaaca tacgggccat ttatccgtaa acgtgtatct tccgttgtta
    24481 aaaaataatc gtagaacgca ttactaggtt caatagtggg tttatcaatt aaaaggtgca
    24541 ctccacgctt aatatcataa tgatccttag ctttagtaac actagtaaga tttacccaag
    24601 cagctggtgg aacatcagtg taagttaatg aaatttgttt aatccggaat tcattacccg
    24661 cataattttc aaggttaaaa tatctaaatt tacttagctc ttcaaatgta tatgtggcac
    24721 ctgaaaaaaa ttcattagtc cggaaaaaaa gaatcctcat agtttgacca acttgtgtac
    24781 gcattttact cgtataagct gcatcagtgg cggtgataac aattttattt atgaaataat
    24841 tattaattgt cggtcgcggg ctaattaaaa acccattacc aagccagtca cctgaacctt
    24901 gtaaactaat ataaccgctg ccctcactaa aataacgcgg tgcaagtaaa taccggccag
    24961 aataaaagtt aatttcaaaa ttatcgtcaa ttacttttga acctgcaaac tcaccatccg
    25021 tgtaaccttg ttcacctaca ttccaagtga cagtaaattc agcggcctca gctttttgtg
    25081 ggctacgtgc cacttgtgtt ttagtaacac taacaccaaa aagaaaagta aaaagtgcta
    25141 aaaaacttaa aaatttattt cttttttgca taataagctc ctatctataa tatactcatt
    25201 tatttaataa cttaaaataa gtcctcacaa caaaaaagta caaaataact aacaatttat
    25261 ggtgaaagat tgaattttta acaaaaaagc ccaagtcttg ataacttgga cttttcggta
    25321 attctaatta acgcttatgc ttcaacgact tgttcttccg ctgcggtttc acgccgttcg
    25381 tcgatttctt tgaccatttg gtcgtacatc ttttcatcaa ttgggtattt acgactgaca
    25441 aggagatagg caagtgtttg acaaataagt ggtaaaagtg ccatccctaa gattaacaca
    25501 aagcgtgctc tgcctaaatt tgcgccgtca attgcggctt gggcgaggtc ttgcacttct
    25561 tcagtgataa gtcgccgtgc gtctccttct ggtaacccgc ctaagacttc gtaatcacta
    25621 attttattag taatatcgta aacaccgccg acagtgagtg ttaaaaacac aacaataacg
    25681 gtgattgcgt tactaatctt cgcggcaaaa ggacgcgcgg caaagacaac accttctttc
    25741 cgttcgccaa atttccattc attatattca accgtatttg taatttgcac gagcagtgta
    25801 agataaaaca caccgtttgg cgtaaaaata atgagcgcta aaacaggtaa aatccattgg
    25861 tagatgctat ttggaatttg taaccaacca aaagcatgtg tacttccaat cacaaagagc
    25921 agtgagttac caattgcgct aatagtgaag gcgccgcgta agatttgtgc tcttgtatat
    25981 tttttcacaa ggaagccaaa aataaagttc gcggcaattg acccaacagc ataccccact
    26041 aagaaataaa agaaagcttg gcccccttca gcgtagccaa aagtaaagtg catatagttt
    26101 tgaccaaggt tattaaacat tgaaccacct aagtaatgaa ggacaattgc cccaagcatt
    26161 actaaaagcg gtttattttg gaagacaact ttaaagatgt ctttaacttt aataatttct
    26221 tcgctatcaa cttcactacg atctcgctct ttacataaga acacaagtaa taattgggag
    26281 aaaattaata cactaccaat aataatacta agtgttctaa atacttgaat cgcgcgtcct
    26341 gggtataaac ttggaacgag tccgccaacg gtaaaagcac caatattaat cgcaatagcc
    26401 atcagcatcg taatttgatt acgttgcttt gggtcacggg taagactagg aaccatcccc
    26461 caatacccaa tatcattaag cgtaaagcta atttcccata agagataagt aacaataaag
    26521 aaaataacat agccccaacc accaaatggt cgaaaactaa agagcaccat gagtaacaaa
    26581 ccgtttagta ccccaccaat taagatccac ggtttatatt taccgagttt aaagcgtgaa
    26641 ttttcaatta acatcccaat aattgggtca ttaaccgcat cccacaagtg aatgaccaac
    26701 atcgtaatca tgattgtcat aatcatcaac ttgtattgtt gaggatcact ttctggtaaa
    26761 gttaattgca agtaaatcat taagaataaa ttaattaatg tatacgctgc atcacgtcca
    26821 atccccgtaa aagaatagac atatttcgtc aattttggga cattatctcc cttataagtt
    26881 ttaaataagt tcagcatata tgattccttt cttatttatg tgagtatata cattttaatg
    26941 atacaacatt attttgatat tatgttcgtt atttagtaaa tatcgtcaaa ttatgttcat
    27001 ta
//
LOCUS       DAOSLP010000011        25083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_2214_length_25083_cov_7.995125, whole genome shotgun sequence.
ACCESSION   DAOSLP010000011 DAOSLP010000000
VERSION     DAOSLP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 25083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25083
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2214_length_25083_cov_7.995125"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..904
                     /locus_tag="PK460_01900"
     CDS             317..904
                     /locus_tag="PK460_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOE53885.1"
                     /translation="MKYGYVRVSTVTQNIDRQMDEMYKLGLSDNEIFIDKQSGKDFNR
                     EKYQELRNILKKNDLLIIKSIDRLGRDYNMIIEEWHYITKIVEADILVIDMPLLDTRN
                     DATNLIGKFISDIVLQILSFVAETERENIKQRQAEGIRLAKERGVHMGRPRYVLPDNF
                     IEVANAYLNREITSNKAAKMVNMARSTFLKYANLL"
     gene            complement(1083..1156)
                     /locus_tag="PK460_01905"
     tRNA            complement(1083..1156)
                     /locus_tag="PK460_01905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1122..1124),aa:Gly,seq:tcc)
     gene            complement(1166..1241)
                     /locus_tag="PK460_01910"
     tRNA            complement(1166..1241)
                     /locus_tag="PK460_01910"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1206..1208),aa:Asp,seq:gtc)
     gene            complement(1346..2008)
                     /locus_tag="PK460_01915"
     CDS             complement(1346..2008)
                     /locus_tag="PK460_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOE53886.1"
                     /translation="MANELDELTGPVNEEGQDINVISKQLKVKVGWGSTLFEVLLWFP
                     LIIPGVIFLFKKIQAKKHFQQLQQRIQHHASQIDNYLEQRVVILNNVVGLVNRAVALD
                     KDTFTTIAAARSGASLDENRNVVAGQVGDVTNKLNIAFENYPELKAHQAIQDAMQQNS
                     YLQREITAARELYNDVVNRWNSDVYAWPTKMIVAAREGYTTRIPFSASAEIKAEARKT
                     FF"
     gene            complement(2055..3902)
                     /locus_tag="PK460_01920"
     CDS             complement(2055..3902)
                     /locus_tag="PK460_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53887.1"
                     /translation="MSKLIFDPLEEYRDKFKGLHDQNVNRFFDELVQKSGINIPENDL
                     TVKEIDNLNIKIDNEEKVLRRVRRIRGFLLFLFIAALIVFILFIVAAVQGEESSLTMS
                     VGARVGIAIGVIVFGIAMLIIVIKVLRPRIKDSQARLSDLISKRDEKIRLAWAQVNPL
                     NELFDANMTPMLVEKTIPLLTMDPFFDSKRLDYLVRKYELSDFTPDNESAEFVQSGEI
                     QGNPFILARTLVMNMGTKTYTGTLTVSYTVTVYVNGKAQRQTRTETLRASVNKPCPYY
                     NNDTYIIYGNEAAPNLIFSRYPQLPLDWTEKSLRCFVEKEEKKMKKLSEKALKQGKQF
                     TPLGNTEFEALFNAYDRNNELEYRLLFTALGQSEILDLIKDKKVGFGDNFQFFKNKNL
                     NLVRSKHSQAFDYSGNPRNYYHYDNKEIRKRFVDYNNNYLHRFYFNIAPVLAIPLYQQ
                     HKPHEFIYQTEYKSHLSFYEHESTVNQFPSSAFAHPDSHTQNILKTKVVQKGKDFDTV
                     SVTSYGFTTVERVDYVPKMAGNGTIHNVPVHWTEYIPVEKESHATIKVLDEESVKKTQ
                     NESFFDKIGEYLSKYTPDQKPVTGRHVMAFLVASKFADDDNEGLDKLFK"
     gene            complement(3963..4271)
                     /gene="trxA"
                     /locus_tag="PK460_01925"
     CDS             complement(3963..4271)
                     /gene="trxA"
                     /locus_tag="PK460_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009872735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOE53888.1"
                     /translation="MIKYLENAADFKEIISKGTVLVDFYADWCGPCRMLGPVLEKLSK
                     ERTDVTVLKVDVDTFGALSQQFSVFSIPTMILFKDGKPVGKQVGYKAFSQLVDFIDNN
                     "
     gene            complement(4281..5111)
                     /locus_tag="PK460_01930"
     CDS             complement(4281..5111)
                     /locus_tag="PK460_01930"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOE53889.1"
                     /translation="MQANRHLILNSFTLKIIAMSAVLIDHVALLFINPTLTIYILMRI
                     IGRIAFVLYAFFISEGVIHTKNTNKYLLRILYLYLIIQALIIGVLIYDPVIELRNIFA
                     TLGAGASLLIYFEKKEWKKVYYLLPFIIVSTINLLAYFTKDSWFIAFSGDNGFFGLAL
                     IIAFYVARKFSIYLLKKYPPSDNTAGEDAEKSAQHVYNVASCVSLLFVASIWYILSVY
                     NSEIPHVALQSFSLISIPLLLLYNGKLGHNSRSWRIIYYSFYPVHVVMLALLFFIIKL
                     "
     gene            complement(5098..5922)
                     /locus_tag="PK460_01935"
     CDS             complement(5098..5922)
                     /locus_tag="PK460_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOE53890.1"
                     /translation="MEEQEKRVDEVNQSEVEVMLEPLNYGSNHHEESRIKSPGFGYGL
                     FFSSLILLFLASFTIDFIVDALVGEGNEVTAFAYKNFGTYVVTFSLITVFLFLTKTLL
                     PLVKKFTKIRPYIHGLFYGFIVIIISVITTLLMQILFGQAEENLNQQIINLILSKHPV
                     SSFFWIVLLGPLVEELTYRVGLFNAISSRNRIAAYVVSALVFGFLHFNIPLNADGAID
                     QAKLIEEFINIPSYIVSGLVFGYIYEKEGFAGSSVAHITNNLISYILSIISIYASK"
     gene            5999..6601
                     /locus_tag="PK460_01940"
     CDS             5999..6601
                     /locus_tag="PK460_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOE53891.1"
                     /translation="MGYVINKSYINEIVVEKSRFIALLCPLTNENDFDEYFKESKTTY
                     KDARHYTYAFRYGNLERASDDGEPQNTAGLPLLNLLKNHDLVNTALIVIRYFGGKKLG
                     AGRLLRTYVDAGNRVINIADKFLEIPAFKYEITIPISDFERTRHYLEKDGFMIDDTDF
                     SSLNVKVLVRVSKDQSESFENLYNVVNKSESVMLKECNDE"
     gene            6591..7358
                     /locus_tag="PK460_01945"
     CDS             6591..7358
                     /locus_tag="PK460_01945"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOE53892.1"
                     /translation="MMNKIKKENIKHIIGIVSGKGGVGKSYVTGSIAVELAKQGYRVG
                     ILDGDVTGPSIPYMFNITSGVYGDEEIIYPATTEKLGIKIISSALLLAHGEDPIMWRG
                     PLIGSLVTQFYTTVNWGELDFLLIDFPPGTSDVALSAFQTIPVDGIVLVTTPQQMVQQ
                     IVKKTYKMGEEMKVKLLGVIENMAYVKLPNSDEKYYLYGQNKLEDLQNTFPINILGSL
                     PFLPQNTKLVDEGKIEVTDTTEFSAIVEKLIKELQNE"
     gene            7351..8562
                     /locus_tag="PK460_01950"
     CDS             7351..8562
                     /locus_tag="PK460_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOE53893.1"
                     /translation="MNKYSNRRQKLYKELANNSALILFSGCEIKKSADGFYDFDVNKN
                     FFYLTGIKQEGSILVVTKIKNIVKETILTSPYDAHKEIWTGRRLKPENVREITGINEY
                     KTTDEFNALLQTLKGNVKTVYLDLEPGIFTHTGLLVSNFREEIINTVNVKVENVYPLI
                     VRLRMVKDEEEIELIRESIALTKKALDFAISKVKPGAYEYEVAAAFEYKIKAAHGVLG
                     FSTIAATGDNAVILHYPDLNTKLEAGELLLLDLGGHNNMYTADISRTVPVNGKFTPLQ
                     ITIYEIVLACNKAVIDFIKPGKTIADLQNYTREFLKERLVKAKLMDKDEDISNYYYHG
                     VSHHLGLDTHDPADRALPLEKGNVITVEPGLYFAKYGIGVRIEDNVLVTEKGSENLSI
                     SIPKEIKEIIS"
     gene            8562..10046
                     /gene="glpK"
                     /locus_tag="PK460_01955"
     CDS             8562..10046
                     /gene="glpK"
                     /locus_tag="PK460_01955"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOE53894.1"
                     /translation="MAQYILTIDQGTTSTRAMLFDKNAEIAFIAQEEIKCYYPKPGYV
                     EQDAIELWLSVLSVINQVLIKANLTFHNITAMGITNQRETTIVWDKNTGMPVAPAIVW
                     QSRQSADICNALSKHQNMIHKKTGLLINPYFSASKIRYILDNLPNGQERADAGELMFG
                     TVDSWLIYKLTNETRHVTDITNASRTMLFNINTLEWDDDILKLFNIPRSMLPEVLASA
                     DYFGETSYFHNTIPITGVAGDQQAALFGQMCLKPGDAKNTYGTGCFMLMNIGDKVQLS
                     KNGLLTTVAWKINGKVTYALEGSVFMGGASVQWLRDQLKLFKNAADTENMAYKAQQND
                     SIYFVPAFVGLGTPYWDDNARGAVFGLTREAGRHELARATLEAIAYQSRDVFDVMIKE
                     SKQDLKVLSVDGGASVNNYLMQFQADILNTTIRVPKIYETTSLGVCYLAGLHTGFFSS
                     IEAITKIHEYTRTYEPKMEEKVRERKYAGWKKAVRAVLTFSEEE"
     gene            10176..12503
                     /locus_tag="PK460_01960"
     CDS             10176..12503
                     /locus_tag="PK460_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOE53895.1"
                     /translation="MNDLVKRLKGYDYVIVDYATKLELLKLRIRNPLLTFKIFTVEEL
                     ITKFLGSASDLSLFALLEKYNNLTYEHAKIINNTIVFPNVSKSSLKVNTLSTYQKALK
                     ALNLLESDFLIAHSLHNKKILLPKYLASNYLLINLLNSVHANFEFTEDMHNFIHEITV
                     FHTGVDEVYAALNRIAALLANGVKPSFIKILKPNSAYEELLRKLAPQFNIVLGPRLFP
                     LNAFSDVVAFVEQFKGQRIDISDPLIQTLRMSTSAPLIALYNLIIAIDFNSVSNKHHY
                     AILKDKIKSESIALEDNDGIEFINEIPLFSANDTYYFYLNFANGAAPNIQSLNPYLNN
                     EERKQLGLLDDETIALINENKLISTLNNNKNIYISFAKIFGQENYELSTLADKLEYEC
                     IEHQLAHKIYSQKYFNYLTGVSYDKLIFYKKHDENFDELFIANDYFDDYGSFDYKFKG
                     INHYHEMVYLSPTKIDLYQRLPFDYFVQHLLNIVDDRPSIHLHFGEFVHSVLENSTSE
                     ETFKVCFSHYLDQMNFNTKDRFFVENERDLIAEAFRFNQRYLNTVMPTKIHNETLIYA
                     PFAAKCVLNGRLDRILLFEKDEKKYIFVVDFKTGNATTNPKNYEYGLDLQLPLYGLLL
                     SLTEDYKDYEVSALVLNSIKLTSTYSAGSRETFTEAIINDIKYTGILIDDADSLKILN
                     AEADSERPFFKIGGRGKVKKLTDLIRREEYKNYMEVTRNKVEETYVGILNNDFRIEDK
                     KINNESSAKYSRYKEISFLPRNSVIEEADYEEGNE"
     gene            12484..15609
                     /locus_tag="PK460_01965"
     CDS             12484..15609
                     /locus_tag="PK460_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE53896.1"
                     /translation="MKKETNKNTITFSPKQEEALSLNKENILVSAGAGSGKTAVLVER
                     IIRILIDKEVPLERILVLTFTRPGAQEFKNRIKKSLLENKTHAHKAFAVDNADITTFD
                     AYSLKILRKYGYLHPTMQSDITNLDANFEMVLMKQYFDEIMAEYYENPPLSLRYFATR
                     FLSKDDRQLFELIKSLDQQVNLQIDPEAYLDNYLDSFYSDTKFEEIYKELDAYVVTTI
                     RKVANAIKHFECERHLEAYETFLDHYGAILSFKDYMDNLDKFTYQRRPSGSLKEHPED
                     LPHAEEVKIYLNDLKEVQVFESKDRMYDVFSENKGHAAFVIEIYSQIRAKLAAYKKEK
                     QAYTFSDIARGAFELVSIPEIQHELKNQYDYILIDEYQDTSDIQEQFINKIANNNVYM
                     VGDIKQSIYGFRNANCDIFREKYARYRKNEGGKKIDLNDNYRSRREVLQSINAILSEI
                     MTEEYGGANYKKEHVINSGNLKYDEFKEERQKVGLEVINYVVPKKNKKNRDNNVLIRD
                     KIDYELDYIINDIEHKIVNKYQVYENKVGMRDVRYGDFTILIAQGTKFNRIEERFIRA
                     GIPVYVDRDEEANENNINIAINKLLTIYVAYTKSDEPKGFEFRLAVASFLRGFVNKYS
                     DQQLFDLLKDDNWDFSGDPILARIKKLAQTTKLLPVTEIIERLITEFDVYGALYNIRD
                     LTQNTMKLSSKLEEIENLAALGLDLEAIVNYFKEREIAGVNAALKRVKLEHDAVTIMT
                     IHRSKGLEFPIVYYMDLESNFYQVSRSQVMKVCPKYGFILPEPSYKSQKTFIGFLSNI
                     YTSKETLSEKIRLLYVALTRAKELMVVIHSTNEDGEPYKTFEKSSNLKQLLLQSTYYD
                     QKRLVVEPEYTQQRLSESEHSKEVKYIYSLHEHFYKFNKKPKTYTVSEIVDVDERLLA
                     RGTLLHRYFEEYDFTTKDLNYISDERDRQHMLNLLSLPFFANANANNIFREYEFIDDG
                     TNKTYAIDCFIIQDDKIMLFDFKLSNIYHPSYDVQVRNYAHYLQKVFKKSVEGYLISI
                     TDLKVRKVSNE"
     gene            15602..16690
                     /locus_tag="PK460_01970"
     CDS             15602..16690
                     /locus_tag="PK460_01970"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOE53897.1"
                     /translation="MNDKKTYTPISNENFLRLLRFYKIPESAEDEVLYNLYIETVELL
                     TLHHQTFENIPYINLDHQRLILQLIHDYDFRMRGLNFEERRSLLKDELFHNKLINVVV
                     DKYGSSAIFKYDSGTYLTPFSMEISTINVYLNFIMLKLGSIPRHNKATELYAELLTSA
                     FSYVLTITELLVRGFEKEALATWRSLHELEATLLLIQDEKVLAQYNQHILYALAFNKL
                     IAQAESDKVFIEIKAKMKDLKLKSKDTKRFIEYGWLLAHNDFDLNIHKFNFRDGVQTL
                     AGLNHKRDIYQIASEVTHSSPLTLFTKRHYFLSIALENLYSSFLTIEVLFAAFYIKNT
                     TKNEAEFYEVTRSIYLEDINFVKDRITK"
     gene            complement(16741..18399)
                     /locus_tag="PK460_01975"
     CDS             complement(16741..18399)
                     /locus_tag="PK460_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOE53898.1"
                     /translation="MENKKTKTFTVVYNGKTTTHDEPVRILDIVGPDKNLICAYVNGR
                     VRELTFLLNRDAILETKTLDNGEGIRIYQTSLRFVAAMAMKRIRPELNVRFSYNVSRS
                     IFIQITNKGVTVDRLLVEKLQREMRNIVEADLPFNRTKITIEEAEKLYEELGYKDKLE
                     ILKYRPEPTVHIYECDGYENYMYGYMVPSSGYLKNFIIRPYSPGMLLQYPRSETKGEI
                     PEFKDAPMFGKALKASHNWAKLTNLDSVAHINHYIENGHIVELINIAEAKHYQMLSEL
                     GQRILEDSEDIRLICIAGPSSSGKTTFANRLRIELMSRGLNAIRLSIDDYYLPRELAP
                     RDEYGNYDLESIYALDIELFNKNMLDLINGEEVELPKFDFKKGQRVPGRKLKVAANQP
                     IIIEGIHALNEQLTSYIPKHQKYKIYIAPQAQINIDEHNPISLTDLRLLRRIVRDKKY
                     RNASAKETMAMWPSVRAGEFKWIYDTQEEADYVYNSLLSYELAVMKKHALPLLLEFDY
                     LSEFFPIAERLIRMLKYFLDMDEKWVPSNSLLREFIGGSCFEDV"
     gene            complement(18491..19249)
                     /locus_tag="PK460_01980"
     CDS             complement(18491..19249)
                     /locus_tag="PK460_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOE53899.1"
                     /translation="MKKEKILQLLLFIYIFSSFLSGCKVSNPNQILVPKRLETTITSE
                     NYTYNMTKAYHEAFTKGKKSFVLYFGSHRCQYCAEIEPLLLNYVQKTKIEIYYIDLYS
                     QEYGDNREYYIETTNVMGAPNILIYKEGEMVHRERGASNLSTQLEVNKFFKEHVRTNN
                     YYTTDKKDNIKPDFKKYVTFTYNFNNATHHELINTKFFPYFEKKGYVNYLVNEDTPLD
                     TPTLSYGSSNKEISLPNDDTELETIISNYESYFS"
     gene            19316..19861
                     /locus_tag="PK460_01985"
     CDS             19316..19861
                     /locus_tag="PK460_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOE53900.1"
                     /translation="MNIWHDMDPRRITTERFDAVVEISRGQQSKYELDKETGLIRLDR
                     VLYTSTHYPQNYGFIPRTYAGDDDPLDVLILCSQPLVPMTIVTCTPIGAVRMLDGGEL
                     DEKIIAIAANDPTYSSYASVDEIPQHVLQEIRHFFQVYKTLEGKETVVHHIQGRDKAI
                     DIIEKSIEMYNEVFNIKQAEE"
     gene            19861..20364
                     /locus_tag="PK460_01990"
     CDS             19861..20364
                     /locus_tag="PK460_01990"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOE53901.1"
                     /translation="MLNIILYQPEMPANVGNIMRTAVAIDATLHIIGPTPIKLDDKSL
                     KRAGMDYVQDLSFFIYDSIDKFMEKHHDKNIFFVTRYSKNNYTEVDYGRFDAGIYVMF
                     GNESYGLPMELLRKHKENTLRIPMVATARSLNLSNAVAVVSYEILRQQGFYNLATSEV
                     IKGDDYL"
     gene            complement(20355..21731)
                     /locus_tag="PK460_01995"
     CDS             complement(20355..21731)
                     /locus_tag="PK460_01995"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOE53902.1"
                     /translation="MQRLFPNDLQQLINANKHFEGLFLVRIRQLSLVDFVFEFSHGNN
                     LFVSLNSPTPFIVFNADLSDFPEGGELHSFAINLRQFLGGNLVAMNQPNDDMILSLVF
                     EQRNNVLELEEVTLFIELIPRHPQAVLVNNKGRILAAYRYHQERAKDGRFIRGGSKYS
                     LPEKGAFSPQISNAKTDYLQAYLKENRERIKKQNFRDVYVYLETNIKRSKRLIRNYEQ
                     DLEKLKELPDLYHKANMLLTYKPAISYYFVQIEGETIEVDPRFDALFNAEILFKKAKK
                     IKKSENILMSKIAETKEKIKYLESIENHLKSLESAGEIFQIYEELGLLKRRDKRQIIS
                     KLNPYVITYKDIDILFGKNNKQNDFLTFKIAKKNDTFMHIQNRPGAHIIIRQENPAKD
                     VLEFAGMLCLFLAKQIDGEVMYSPIYNIKKGAFPGQVLTRKYNTFFLRFNDDLKPFFE
                     NNIKRFYK"
     gene            21820..22392
                     /gene="gmk"
                     /locus_tag="PK460_02000"
     CDS             21820..22392
                     /gene="gmk"
                     /locus_tag="PK460_02000"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOE53903.1"
                     /translation="MRQGFIVLISGPSGVGKGTLIHSLLADQTLNLSFSVSMTTRKPR
                     PNEKDGIHYFFVSHETFLKNIEENNFLEHAQFVGNRYGTPRDYVEKLLNEGKNVLIEI
                     EVDGARQLLKNVDSEKVLSLYIMPPSVQELVRRLRLRGSEDEATIQRRAERFKEEIKY
                     KDQYQHIIINDELETCINDVKALIREKMNI"
     gene            22395..24584
                     /gene="priA"
                     /locus_tag="PK460_02005"
     CDS             22395..24584
                     /gene="priA"
                     /locus_tag="PK460_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOE53904.1"
                     /translation="MQLIEVFTQYSKLNRPFTYYYDGDDLEVGVRVLIDFNNREIVGY
                     VAAVKSTKETIETASKRLGFELAEITKILDEKPLLTNELMELSENVTSYYLTNRISVL
                     NAMLPPSLRPSKGSLSAPKIAMQKKVSFNPEFTDFDKLTDKQSSLLSEIMTHGSVLQT
                     SKNRTQIMQLLNKGAVIVEEIEKYRLRYEDYPREDPHELTNLQKEAVKSILEDYKETF
                     LLHGITGSGKTEIYLALAAHYLAQNKQVIMLVPEIALTGTMLANFYRRFGKKIAILHS
                     ELTPAEKYDEYRRISAGEVDIVVGARSAIFAPLQNIGLIILDEEHAETYKQDVAPFYH
                     ALTVAQMRAKSFNAKVLVGSATPSIETMARARKEVYGYYYLGERINLQPLPETTVVNM
                     LDYKNVTTTSPYISKLLATEIRKAIDAKTQVILLVNRRGYATFVSCRECGESKKCPAC
                     AISLTYHKVGNNLKCHHCGLSFPKNDPCIKCGSTNFSYTGFATQKAEEDIQKMFPDAR
                     ILRLDSDVSRKDKTPQVVLNKFMNHEADILIGTQMVSKGHDFPLVSLVGVIGTDANLS
                     IPSFRSSERTFQLITQAIGRSGREKIKGKAIIQTMMPEHYVIQHAKQQDYFGFYEVEI
                     RHRYLTSNPPFYFLLSITMSHQNERTVKDTILTLKLNLIKALGEDATVIGPTATFYPA
                     SGKTFNETLIVRYKNYFKIKPALLTTLSPFREQSSLYVHINVDPYDV"
     gene            24599..25039
                     /locus_tag="PK460_02010"
     CDS             24599..25039
                     /locus_tag="PK460_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53905.1"
                     /translation="MVIIKIYSQDIYVAAEISVKYADAIAKTLGLTKEDVFLLGSDGA
                     LIAEGVDQVSWFTYLEVTIPANLKHKQDALSKTLLEIFNNYGVHLMIKYDYVTKDNIV
                     KILNPDFKVFNETMVEVLPTGNYYDEDDVDWDDDWDEDEDKEVR"
BASE COUNT         8621 a         3926 c         4415 g         8121 t
ORIGIN      
        1 taattattat ttttatcccc taaacttttc tagtgtataa ccctaaagac gggaaatcat
       61 cctttccttt cttttaattg ttttgcgaca aaaaggtacg cctttatgga ttttggaaaa
      121 gattgttaaa atattttatt gtttcaaata aagcctgaaa attattttta atcgttgact
      181 gagtggaatt tttatataat ttaggttaaa atgtaagcag atataaaggt gtggctttat
      241 gaccgacagt tgattcttac aactgtcttt tttcataaaa atgaggggta taatacttta
      301 aaaaggagtt gtttttatga aatatggata tgttagagtt tcaactgtta cacaaaatat
      361 tgatagacaa atggatgaaa tgtataaact tggtttaagt gataatgaaa tttttatcga
      421 taaacagagt gggaaagatt tcaatcgtga aaaatatcaa gaactaagaa acattttaaa
      481 gaaaaacgat ctattaataa taaaatcaat tgatcgttta ggtagagatt ataatatgat
      541 aattgaggag tggcattata ttactaaaat agttgaagca gatattttag taattgatat
      601 gccactttta gatacaagaa atgatgcgac aaatttaata ggtaaattta ttagtgatat
      661 tgtcttacaa atactttcct tcgttgccga aacggaaaga gagaatataa aacagagaca
      721 ggctgaagga ataaggcttg caaaagaaag aggcgtccac atgggacgcc ctagatatgt
      781 attgcccgat aactttattg aagttgcaaa tgcttattta aatagagaaa taacatcaaa
      841 taaggctgct aaaatggtta atatggctag aagcacattt ttaaaatacg caaacttatt
      901 ataaaatgta attcggttcg gacacctgtc gagtttgcaa aagtcaatcg gttcggacac
      961 ctgtcgcgtt cagtaatttt taacatgaaa aaagcctcag aaacacgaat tcttctaagg
     1021 cttataaatt tctataatcg ctttgttggc ttaataaagc ccttactaaa gtaagatttt
     1081 tttggagcag gtgacgggaa tcgaacccgc gtatttagct tggaaggcta atgttctacc
     1141 attgaactac acctgcatac gtcactggcg gaccagacgg gactcgaacc cgcgatcttc
     1201 tgcgtgacag gcagacatgt taaccactac actactggtc cattgcttta ctattatatt
     1261 aaaattacga ttaatatcaa gtcttattat ttagaaaaag aaaaaccaac ggttaggttg
     1321 gtttttccta tctacaagat tctttctaga agaaagtttt acgagcttca gctttaattt
     1381 ctgcagatgc tgagaaagga atacgagttg tataaccttc gcgcgcggca acaatcatct
     1441 ttgttggcca tgcgtaaaca tcactattcc agcgattgac gacatcgtta taaagttcac
     1501 gagcggctgt aatttcgcgt tggagatagc tgttttgttg cattgcgtct tgaattgctt
     1561 gatgggcttt gagttctgga tagttttcaa aagcgatgtt aagtttatta gtgacatcac
     1621 caacttgtcc ggcgacaaca ttacgatttt catcaaggct agcaccacta cgagcagcgg
     1681 caattgtggt aaaggtatct ttgtcaagtg cgacagcgcg attaactaaa ccaacaacgt
     1741 tattaagaat aacgacgcgt tgttctaagt agttgtcgat ttgactagcg tgatgttgaa
     1801 tgcgttgttg taattgttgg aagtgttttt tggcttggat tttcttaaat aagaagataa
     1861 ctccaggaat aataagtggg aaccaaagta acacttcaaa gagtgttgaa ccccatccaa
     1921 cttttacttt tagttgttta ctaattacat taatatcttg accttcttca ttaactggtc
     1981 cagttagttc atctaattca tttgccatag tatttttctc ctttctttaa ttatactatt
     2041 aataaataaa cttattactt aaataatttg tcaaggcctt cattatcgtc atcagcgaat
     2101 ttacttgcca caaggaaagc cataacatgg cggccggtga ccggtttttg atcaggggtg
     2161 tatttactta aatattcacc aatcttatca aagaatgatt cattttgtgt tttcttcacg
     2221 ctctcttcat caagtacttt aatcgttgcg tgtgattctt tttcaactgg gatatattca
     2281 gtccaatgga cagggacatt atgaatagtg ccatttcctg ccattttggg aacatagtca
     2341 acgcgttcta cagtagtaaa accgtagcta gtcacactga cggtatcgaa gtctttacct
     2401 ttttgcacaa cttttgtttt taagatgttt tgtgtatgac tatcagggtg cgcgaaagca
     2461 ctacttggga actggttcac cgttgattca tgttcataaa atgaaaggtg cgatttatat
     2521 tctgtttgat aaataaattc atgtggttta tgttgttgat aaagcgggat tgcaaggaca
     2581 ggagcgatat taaaatagaa acgatgcaaa taattattgt tataatcgac aaagcgtttt
     2641 ctaatttctt tattatcata gtgataataa ttacgtggat tcccactata atcaaaggct
     2701 tgtgaatgtt tagaacgaac aagatttaag tttttgtttt taaagaattg gaagttatca
     2761 ccaaacccaa ctttcttatc cttaattaaa tctaatattt cactttgacc taaagcagta
     2821 aataataagc gatattctaa ttcattgttg cggtcataag cgttaaagag tgcttcaaat
     2881 tcagtattac caagtggtgt aaattgttta ccttgtttta gtgctttttc actaagtttt
     2941 ttcatctttt tttcttcttt ttcgacaaag catcgtaaac tcttttctgt ccaatcaagt
     3001 gggagttgcg gataacgact aaagattaaa ttaggcgctg cttcgttacc ataaataata
     3061 taagtatcat tgttgtagta tggacacggt ttattcacac ttgcgcgcaa tgtttctgtg
     3121 cgtgtttgtc gttgcgcttt accgttaaca tagacggtaa ccgtataaga aacagtaagt
     3181 gtcccagtat aagttttagt acccatattc atcacaagcg ttcttgctaa aataaatgga
     3241 ttaccttgaa tttcacccga ttgcacaaat tccgcggatt cattatcagg agtaaagtca
     3301 cttaattcgt atttacgcac taagtaatca agccgcttgg aatcaaaaaa tggatccatt
     3361 gtaagcagcg gaattgtttt ttccactaac attggtgtca tatttgcgtc aaacagttca
     3421 ttaagcggat taacttgtgc ccatgctagg cgaatttttt cgtcacgctt actaattaag
     3481 tcacttagtc tagcttgtga atctttaatc cgtggtcgta atactttgat gacaattatg
     3541 agcatagcga taccaaatac gattacgcca atggcaatcc ctactcgtgc accaacgctc
     3601 atcgttaggg aactctcttc accttgcact gcggcaacaa taaataaaat aaaaactatt
     3661 aaagcggcga taaataaaaa taagagaaac ccgcgtatac gccgtacgcg acggagtact
     3721 ttctcttcat tatcgatttt gatatttaaa ttatcgattt ctttaacggt taaatcgttc
     3781 tcaggaatat taatgccgct tttttgcact agttcatcaa aaaaacggtt tacattttgg
     3841 tcatgtaacc ctttaaactt gtcacgatat tcttcaagtg ggtcaaaaat taatttgctc
     3901 atagattgat tataacaaag attaaaaaat tgtaatttat tttatgataa aaatttcaaa
     3961 ttttagttat tatcaataaa gtccactaat tgtgaaaagg ctttataacc aacttgttta
     4021 ccaaccggtt tgccatcttt aaaaagaatc attgttggaa tcgaaaaaac actaaattgt
     4081 tgggagagtg ctccaaaagt gtcaacatca acctttaaaa cagtgacatc agtgcgttcc
     4141 ttacttaatt tttcaaggac tggaccaagc attcggcacg gaccacacca atcagcgtaa
     4201 aagtcgacta agactgttcc tttactaata atttctttaa aatctgctgc attttccaag
     4261 tatttaatca taagtgcctc ctagagttta ataatgaaga atagtagtgc aagcattact
     4321 acatgaacgg gatagaaaga ataatagata attcgccaac tacgactatt atggcctaat
     4381 ttaccgttat agagtaacaa gagcggaata cttataagtg agaaactttg caatgcaaca
     4441 tgcggtattt cgctattgta cacgcttaaa atataccaaa tggacgcaac gaataaaaga
     4501 gaaacgcaac ttgcgacatt atacacatgc tgtgcgcttt tttcagcatc ctctccagcg
     4561 gtattatcac taggcggata cttcttcagt aaatagatac tgaatttacg cgcgacatag
     4621 aaagcgatga ttaaggctaa accaaagaag ccgttatccc cggaaaaagc aataaaccac
     4681 gaatctttag taaaataggc taataaatta attgtcgaaa caataataaa cggtaataag
     4741 taatacactt tcttccattc ttttttctcg aaatagatga gtaaacttgc accagcgcca
     4801 agcgtagcga aaatatttct tagttcgata actgggtcgt aaattaaaac gccaatgata
     4861 agtgcttgga ttattaaata taaatataaa atgcgcaata agtatttatt ggtattttta
     4921 gtatgtatca ctccttcact tataaaaaaa gcgtaaagta caaatgcaat gcgcccaata
     4981 atgcgcatta ggatataaat tgttaatgtg ggattaataa aaagaagcgc aacatggtca
     5041 attaaaactg cactcattgc gataatttta agcgtgaatg aatttaatat taaatgtcta
     5101 tttgcttgca taaatactaa taattgaaag aatatacgat attaaattgt tagtgatatg
     5161 ggcaacactt gacccagcaa acccttcttt ttcataaata tagccaaaga cgagtccgct
     5221 aacaatgtaa ctaggaatat taataaattc ttcaattagt ttagcttgat caatagcgcc
     5281 atcagcgtta agtggaatat taaagtgtaa gaaaccaaac acaagtgcgc taacgacgta
     5341 ggctgcgata cgattccgtg acgatatcgc attaaataac ccaacgcgat acgttaactc
     5401 ttccactagt gggccaagta agacaatcca gaaaaaggaa ctgacaggat gcttcgataa
     5461 gattaaatta attatttgtt gatttaagtt ttcttcggct tgaccgaata gaatttgcat
     5521 taaaagtgtt gtgattactg aaataattat cacaataaat ccataaaata aaccgtgtat
     5581 gtatgggcgt attttagtaa acttcttaac aagaggcaaa agtgttttag ttaagaataa
     5641 aaaaactgta attagcgaaa atgtaacaac ataggtacca aaattcttat aagcaaacgc
     5701 tgttacctca tttccttcac cgactaaggc atcgacaata aaatcgatag taaaactagc
     5761 gaggaataaa agtataaggc tactaaaaaa taatccataa ccaaaacccg gtgatttgat
     5821 tcgtgattct tcgtggtgat tacttccata attaagtggt tcaagcatga cttcaacttc
     5881 actttgattt acttcatcga ctcttttttc ttgttcttcc atacataaag attataacat
     5941 ttacttattt tttgcgttag taaagcataa atatcataat tcttgataga attaaactat
     6001 gggttatgtc ataaataaaa gctatattaa cgaaatcgtt gtcgagaaat cacgcttcat
     6061 tgcgctttta tgtccgctga ctaatgagaa tgattttgat gaatacttta aagaatcaaa
     6121 aacaacatat aaagatgcta gacattatac ttatgctttt aggtatggga atttagagcg
     6181 cgctagtgat gatggcgaac cacaaaacac agcaggatta ccgctgctca acttactaaa
     6241 gaatcatgat ttagtgaata cagcgcttat tgtaatccgt tatttcggtg gtaaaaaact
     6301 tggagcaggt cgtttactgc gcacatatgt tgatgctggg aatcgcgtga ttaatattgc
     6361 tgacaagttt ttagaaattc ctgcttttaa gtatgaaata actataccaa ttagtgactt
     6421 tgaaaggaca cgtcattatt tagaaaaaga cgggtttatg attgatgaca ctgattttag
     6481 tagcttaaat gtaaaagtgc tagttcgtgt tagtaaagac caaagtgaat cgtttgaaaa
     6541 tctttataat gtcgttaaca aaagtgaaag tgttatgtta aaggagtgta atgatgaata
     6601 agattaaaaa agaaaatatt aaacatatca tcggtattgt ttctggtaaa ggtggtgtag
     6661 gtaagagcta tgtaacgggt tcaattgctg ttgaactcgc gaagcaaggc tatcgtgttg
     6721 gaattttaga tggtgacgtt acaggtccct caatccctta tatgtttaac atcactagcg
     6781 gggtttatgg tgacgaagaa attatttatc ctgctacgac tgaaaaatta ggaattaaga
     6841 ttatttcaag cgctttgctt cttgcgcatg gtgaagatcc aattatgtgg cgcggaccac
     6901 tgattggtag tctagttaca caattttaca caactgttaa ttggggtgaa ttagactttt
     6961 tactcattga tttcccacca ggaacaagtg acgtcgcact tagtgctttt caaactattc
     7021 cagttgacgg gattgtgctt gtgacaacac cacaacaaat ggtccaacaa attgtgaaga
     7081 aaacatataa gatgggggaa gaaatgaaag tcaaacttct cggtgttatc gaaaatatgg
     7141 catatgttaa attaccaaat agtgatgaaa agtattattt atatggtcaa aataaattag
     7201 aagacttaca aaacacattc ccgattaata ttttaggatc acttcctttc ttaccacaaa
     7261 atacaaaact tgttgatgaa ggtaaaatag aggtaacaga tacgactgaa ttcagtgcaa
     7321 ttgtcgaaaa attaataaag gagttgcaaa atgaataaat atagtaatag acgccaaaaa
     7381 ttatataaag aacttgcaaa taatagtgcg ctcattcttt ttagtggttg tgagattaag
     7441 aaaagcgccg atgggtttta tgatttcgat gtaaataaga acttctttta cttaactggt
     7501 attaaacaag aaggttcaat tttagtcgta acgaaaatta aaaacatcgt gaaagaaaca
     7561 attttaacga gtccatatga tgcccataaa gaaatatgga caggtcgccg gttaaaacca
     7621 gaaaatgtaa gagaaataac tgggattaac gaatataaaa caacagatga atttaatgca
     7681 ttgctacaaa cattaaaagg taatgtaaaa acagtttatc ttgatttaga acctggcatt
     7741 ttcacgcata ctggtttatt agtaagcaat ttcagagaag aaatcattaa taccgttaat
     7801 gtaaaagttg aaaatgtcta cccgttaatc gttcgtttgc gcatggtaaa agatgaagag
     7861 gaaattgaac ttattcgcga aagcatcgct ttaactaaaa aggcacttga ttttgccata
     7921 agtaaagtaa agcctggcgc ttatgaatat gaagttgccg ctgcttttga atataaaatt
     7981 aaagcggcgc atggtgtttt gggctttagt actatcgccg ctacaggtga taacgcggta
     8041 attcttcatt accctgactt aaacactaaa cttgaagcag gggaattatt actccttgat
     8101 ttaggtggtc ataataatat gtacaccgcc gatatatcgc ggactgtgcc cgttaacggt
     8161 aaatttactc cgttacaaat caccatttac gaaatcgttt tagcatgtaa taaagcagtg
     8221 attgatttta ttaaacctgg taaaactatc gctgacttac aaaactatac acgcgaattt
     8281 ttaaaagagc gcttagtgaa agcaaaatta atggataaag acgaagatat cagtaactac
     8341 tactatcacg gtgttagtca tcatttagga ctcgatacgc atgatcctgc tgatcgcgct
     8401 ttaccacttg aaaagggtaa tgtaattacc gttgaaccag gactttactt cgcaaagtac
     8461 ggtattggtg tccgcattga ggataatgta ttagtgacag aaaaagggtc agaaaattta
     8521 tcgattagta ttccaaaaga aataaaggag attattagtt aatggcacaa tatattttaa
     8581 ctattgacca aggaacaaca tcaacgagag cgatgctctt tgataagaat gcggaaattg
     8641 cttttatcgc ccaagaagaa attaagtgct actatccaaa accaggatat gttgaacaag
     8701 atgcgattga attatggctt agtgtcctta gtgttattaa ccaagtctta attaaagcga
     8761 atttaacatt tcataacatt acggcgatgg gaattacaaa tcaaagggaa acgacgattg
     8821 tatgggacaa aaacactgga atgcctgtag cgccagccat tgtgtggcaa agtcggcaaa
     8881 gcgctgatat atgtaatgct ttaagtaaac atcaaaatat gattcataaa aaaacaggct
     8941 tacttattaa cccttatttt agcgctagta agatccgtta tattcttgat aatttaccaa
     9001 acggacaaga acgcgctgat gctggtgaac ttatgtttgg tactgttgac tcatggttaa
     9061 tatacaaatt aacgaatgaa acacggcatg ttactgatat tactaatgcc tcacggacga
     9121 tgttattcaa tattaatact ttagaatggg atgatgacat cttaaagtta tttaatattc
     9181 cgcgtagtat gttacctgaa gtcttagcta gtgctgacta tttcggtgaa acaagttatt
     9241 ttcataatac aatcccgatt actggcgtag cgggtgacca acaagcagca ttatttgggc
     9301 aaatgtgttt aaagccaggt gatgccaaaa atacatatgg aacagggtgc tttatgctga
     9361 tgaatattgg ggataaagtg caactttcta aaaacggatt actcacaaca gttgcgtgga
     9421 agataaatgg gaaagtgact tatgcactag aaggtagtgt ctttatgggt ggcgctagtg
     9481 tgcagtggct acgtgatcaa ctaaaacttt ttaaaaatgc cgctgatact gaaaatatgg
     9541 cttacaaagc acagcaaaat gatagcattt actttgttcc agcttttgtt gggttaggga
     9601 ctccatattg ggacgataat gctcgtggtg ccgtttttgg cttaacgcgc gaagcaggac
     9661 gtcacgaatt agcgcgcgca acacttgaag cgattgccta tcagtctcgt gatgtgtttg
     9721 atgtgatgat taaagaatca aaacaagact taaaagtttt aagtgtcgat ggtggtgcgt
     9781 ctgttaataa ttacttaatg caattccagg ctgatatatt aaacacaaca attcgtgttc
     9841 caaagattta cgaaacaact tcacttggtg tttgctatct agcgggtcta cacaccggct
     9901 tcttcagttc aatcgaagca attacaaaga ttcatgagta cacacgaact tatgaaccaa
     9961 aaatggagga gaaagttcgg gaacgaaaat atgctggatg gaaaaaggct gtacgcgctg
    10021 tattaacctt cagcgaagaa gagtaacatt aaactattaa ttacgctggt taataagcca
    10081 gcgtttttta tttcttctaa gttatattaa caaaagttaa taaaactgta gaaaaagtat
    10141 tgggggtctt aacttacttt gctataatca aatatatgaa tgatttggtt aagcgtttaa
    10201 aaggctatga ctatgttatt gttgattatg cgacaaaact tgaactttta aagctgcgta
    10261 taagaaatcc acttttaact tttaaaatat ttacagttga agaattaatt acgaaatttt
    10321 tagggagtgc tagtgatcta agtcttttcg ccctacttga aaaatataat aatctaactt
    10381 atgaacacgc aaagataatt aataacacga ttgtttttcc taatgtaagt aaaagtagtt
    10441 taaaagttaa tacattatca acatatcaaa aagccttgaa agctttaaat ttattagaaa
    10501 gtgacttctt aatcgctcat agtttacata ataagaaaat tttattacca aaatatttag
    10561 caagtaacta cttactaatt aatttgttaa acagcgttca cgctaatttt gaatttactg
    10621 aggacatgca taattttatt catgaaatca cagtttttca taccggtgtt gatgaagttt
    10681 acgctgcttt aaataggatt gcagctttac tagcaaatgg agtgaagcct tcgtttatta
    10741 agattttgaa accaaatagt gcatatgaag aattattacg aaaattagcg ccacagttta
    10801 atattgtgct tggaccccgg ctatttccac ttaatgcctt ttctgatgta gttgcattcg
    10861 ttgaacaatt taaaggacag cgaatagata ttagtgaccc acttattcaa acactgcgga
    10921 tgagtacaag tgcaccatta attgctctct acaatttaat cattgctatt gattttaata
    10981 gtgttagtaa taagcatcat tacgctattc taaaagataa gattaaaagt gagtcaattg
    11041 ctttagagga caatgacggg attgaattta ttaatgaaat accgttattt agcgctaacg
    11101 acacctatta tttctatctt aatttcgcta atggtgctgc gccaaatatt caaagcttaa
    11161 atccatattt aaataatgaa gaacgcaaac aattaggttt attagatgac gaaacaattg
    11221 ctttaattaa tgaaaataaa ttaataagta ctctaaacaa taataagaat atttatatat
    11281 catttgctaa gatatttggt caagaaaatt atgaattaag tacattagcc gataaactag
    11341 aatatgagtg cattgaacac caattagcgc ataaaattta tagtcaaaag tattttaatt
    11401 atttaacggg tgtttcttac gataaattaa tcttttataa aaaacatgac gaaaactttg
    11461 atgaattatt tatcgctaat gattattttg atgattatgg aagttttgat tataagttta
    11521 aagggattaa tcattaccat gaaatggtat atttatcacc cactaaaatc gatttatatc
    11581 aacgattacc atttgattat tttgtacaac atttacttaa tatcgtagat gaccggccaa
    11641 gcattcatct tcattttgga gaatttgtcc atagtgtatt agaaaatagt acgagtgaag
    11701 aaacatttaa agtatgtttt agtcactatc ttgaccaaat gaattttaat actaaagatc
    11761 gttttttcgt agaaaatgag cgagatctaa ttgccgaagc ttttagattt aatcaacggt
    11821 atttaaatac agttatgccc actaaaattc ataatgaaac actaatttat gccccttttg
    11881 ctgcaaaatg tgtattaaat ggtcgattag atcgtatttt attatttgaa aaagatgaaa
    11941 agaaatatat atttgttgtt gactttaaaa ctgggaatgc aaccactaat cctaaaaatt
    12001 atgaatatgg tttagactta caattaccgc tttacggact acttctttct ttaactgaag
    12061 attacaagga ttatgaagtt agtgctcttg tattaaactc cattaaactt actagtacat
    12121 atagtgcggg tagtcgagaa acatttacag aagcaattat taatgacatc aaatacactg
    12181 gtattttaat tgatgacgct gatagcctaa aaatattaaa cgctgaagca gatagtgagc
    12241 gaccattttt taaaataggt ggacgcggga aagttaaaaa acttacggac ttaatccgta
    12301 gagaagaata taaaaattat atggaagtga cgcgtaataa agtcgaagaa acttatgtgg
    12361 gtatattaaa taatgatttt agaattgaag ataaaaagat aaataacgaa agtagcgcga
    12421 aatattcacg ctataaagaa attagtttct taccgaggaa cagtgttatt gaagaggcag
    12481 attatgaaga aggaaacgaa taagaacaca ataacattta gtccgaaaca agaggaagct
    12541 cttagtttaa acaaagaaaa tattcttgtt tctgctggtg ctggtagtgg taaaactgct
    12601 gttttagtcg aacgtattat tcgtatttta attgataaag aggttccttt agaacggatt
    12661 cttgttctta cctttactag acctggagcg caagagttta aaaatcggat aaaaaaatcc
    12721 ttgcttgaaa ataaaacgca tgcacataaa gcttttgcgg tcgataatgc agatattaca
    12781 acatttgatg cttactcctt aaaaatatta cgtaaatatg gttatctcca ccccacaatg
    12841 caaagtgata taactaattt agacgctaat tttgaaatgg tgttaatgaa acaatatttt
    12901 gatgaaatta tggctgaata ttatgaaaat cccccgcttt cattacgcta ttttgcgact
    12961 aggtttctta gtaaggatga tcgacaatta tttgaactaa ttaaaagttt agaccaacaa
    13021 gttaatttac aaatagatcc agaagcttac cttgataatt atttagactc cttctattca
    13081 gatactaaat ttgaagaaat ttataaagaa ctagatgctt atgttgtaac tacgatacgt
    13141 aaagtcgcta atgccattaa gcattttgaa tgtgaaagac atttagaagc ttatgaaaca
    13201 tttctagacc attatggtgc gatattatcg tttaaagatt atatggataa cctcgataaa
    13261 tttacttatc agcgacggcc aagtggctca ttaaaagagc atcctgaaga tttaccgcac
    13321 gctgaagaag ttaagatata ccttaatgac ttaaaggaag tacaagtttt tgaatcaaaa
    13381 gatagaatgt atgatgtctt tagtgaaaat aagggtcatg cagcgtttgt tattgaaata
    13441 tattcgcaaa ttagagcaaa acttgcagcg tataaaaagg aaaaacaagc ttatactttt
    13501 agtgatatcg ctcgcggtgc ttttgagtta gtgagtattc ctgaaattca acatgaactt
    13561 aaaaatcaat atgattatat tttgattgat gaatatcaag atacaagcga tattcaagaa
    13621 caatttataa ataaaattgc caataataat gtttatatgg ttggtgatat aaaacagagt
    13681 atttatggtt ttagaaacgc taattgtgat atattccgtg aaaaatatgc tagatatcgc
    13741 aaaaacgaag gcggtaaaaa gattgattta aatgataatt atcgaagtcg tagagaagtc
    13801 ttacaatcaa ttaacgctat tttaagtgaa ataatgactg aagaatatgg gggcgctaac
    13861 tataaaaagg aacatgtgat aaattcgggc aatcttaaat atgatgaatt caaagaagaa
    13921 agacaaaaag taggattaga agtaattaat tatgttgtgc ctaaaaaaaa taagaaaaat
    13981 cgtgataata atgttttaat ccgtgacaag atagattatg aacttgatta catcattaat
    14041 gacatcgaac ataaaatagt taataaatat caggtttatg aaaataaagt cggtatgcgt
    14101 gatgtgcgtt atggtgactt tacaattttg attgcgcaag gaacgaaatt taatcgaata
    14161 gaagaacgct ttatccgcgc aggaattcct gtttatgttg atcgtgatga agaagcaaat
    14221 gaaaataata ttaatatcgc gataaataaa ttactaacta tttatgttgc ttacacaaaa
    14281 agtgatgagc cgaaaggttt tgaattccgt ttagcggtcg ctagtttctt acgcggattt
    14341 gttaataaat atagcgacca acaacttttt gatttattaa aagatgataa ttgggatttt
    14401 agtggtgacc caatactagc acgcattaaa aagctagcac aaaccactaa actcttaccc
    14461 gtaacagaaa ttattgagcg cctcattact gaatttgatg tatatggtgc actatacaat
    14521 attcgagatt taacgcaaaa tactatgaaa ttgtcgagta aattagaaga aatagaaaat
    14581 ttagcggcat taggtttaga tttagaagct attgtaaatt actttaaaga acgtgagatt
    14641 gcgggagtca atgctgcctt aaaacgtgta aaattagagc atgatgctgt aacgattatg
    14701 acaatccacc gttcaaaggg tctagaattt ccgattgtct attatatgga ccttgaatct
    14761 aatttttatc aagtaagtag gagtcaagtg atgaaggtat gtccaaagta cggttttatt
    14821 ttacccgaac cgtcctataa atcccaaaag actttcattg gtttcttgtc aaatatatat
    14881 acatctaaag aaacattatc cgagaaaatt agattacttt atgttgccct aacacgtgcc
    14941 aaagagttga tggttgtcat tcattctact aatgaagatg gagaacctta taaaactttt
    15001 gaaaagtcta gtaaccttaa gcaactcctt cttcaatcaa cttactatga tcaaaagcgt
    15061 ttagtagttg agcctgaata cacacaacag cgacttagcg agagtgaaca tagtaaagaa
    15121 gttaaataca tttattcgct tcatgaacat ttttataaat ttaataaaaa gcctaaaact
    15181 tacacggtta gtgaaatagt tgatgttgat gagaggcttt tagcgcgtgg tactttgctc
    15241 catcgttatt ttgaagaata tgattttact acgaaagatc ttaattatat tagtgatgaa
    15301 agagatcgac aacatatgct taacctgtta agtttacctt tcttcgctaa tgctaatgcg
    15361 aataatatat tccgtgaata tgaatttatt gatgatggaa caaataaaac ctatgcgatt
    15421 gactgcttta tcattcaaga tgataagata atgctttttg attttaaact ttcaaatatt
    15481 tatcatccaa gttatgatgt tcaagtaaga aactatgcac attatttaca aaaggttttt
    15541 aagaaaagtg ttgaaggcta tttaattagt attactgact taaaagtgag gaaagtatca
    15601 aatgaatgat aaaaaaacat atacaccgat aagtaatgaa aactttttgc ggttgcttcg
    15661 gttttataaa attccagaat cagcggaaga tgaagtctta tacaatttat acatagaaac
    15721 cgttgaactt ctcacattac atcatcagac ttttgaaaat ataccgtata ttaatcttga
    15781 tcatcagcga ttaatattac aacttattca tgattatgat tttcggatgc gtggtcttaa
    15841 ctttgaagaa cgtcggtcac tacttaaaga tgaacttttt cataacaaac tcattaatgt
    15901 tgttgttgat aaatatggga gcagcgctat ttttaagtat gattctggca cttatttaac
    15961 tccgttttct atggaaatat cgacaattaa tgtttattta aatttcataa tgcttaaatt
    16021 aggatcgatt ccacgccata ataaagcgac agaactatat gcggaattac tgacaagtgc
    16081 tttttcctat gtccttacga ttacagaatt actcgtgcgc ggttttgaaa aagaagcgct
    16141 tgctacatgg cgctcactgc atgaattaga agcaacacta ctcttaattc aagatgaaaa
    16201 ggtactcgct caatataatc aacatattct ttacgcctta gcgtttaata aacttatcgc
    16261 acaagcagaa agcgataaag tttttattga aattaaagcg aagatgaaag acttaaaact
    16321 taaaagtaaa gatacgaaaa gatttattga atacggctgg cttttagcgc acaacgattt
    16381 tgatttaaac attcataagt ttaattttcg tgacggggtg cagacgctcg ctggactaaa
    16441 tcataaacgc gatatttatc aaattgctag tgaagttaca catagttcgc cacttacatt
    16501 atttactaaa cgacattact ttttaagtat tgcccttgaa aatctataca gctcattttt
    16561 aacaattgaa gtgctttttg cagcgtttta catcaaaaac accacaaaaa atgaagcgga
    16621 attttatgaa gttacgcgtt ctatttatct agaagatatt aactttgtaa aagatcgcat
    16681 aacaaaataa aaaagagcca ctcggttaaa agtggctcaa gagttagcaa ttattaatga
    16741 ttatacatct tcaaaacagc tgccgccgat aaattcacgg agtaatgaat tagaaggtac
    16801 ccacttttca tccatatcaa ggaaatattt aagcatccgg attaagcgtt ccgcaatagg
    16861 aaagaattca cttaaataat caaattcaag caagagcggt aacgcatgct ttttcataac
    16921 cgctaattcg taagagagca atgaattata tacgtaatcg gcttcttctt gtgtgtcata
    16981 aatccattta aattcaccag cgcgaacact tggccacatt gccattgttt ctttagcact
    17041 agcgttacga tatttcttat cgcgaacaat acgacgtaat aatcttaaat cagtaagact
    17101 aattgggtta tgttcatcaa tgttaatttg tgcttgtggt gcaatataaa ttttatattt
    17161 ttggtgtttt ggaatataac tagttaattg ctcattaagt gcgtgaattc cttcgatgat
    17221 gattggttga ttagccgcta cttttagttt tcgtcctggt acgcgttgac cctttttaaa
    17281 gtcaaacttt ggtaattcaa cttcttcacc attaattaag tcgagcatgt ttttattaaa
    17341 taattcgata tcaagggcat aaattgattc taaatcatag ttgccatatt catcacgcgg
    17401 tgctaattcg cgtggtaagt agtagtcgtc aattgataaa cgaatagcgt taagtccgcg
    17461 actcattagt tcaatgcgta atctgtttgc aaatgttgtc ttaccagagc tacttggacc
    17521 tgcaatacaa attaagcgaa tatcttcgga atcttctaaa atacgttgtc ctaattcact
    17581 taacatttgg taatgtttag cttcggcgat atttattaat tcgacgatat ggccattttc
    17641 aatatagtgg ttaatatggg cgacactatc taaatttgtt aatttagccc agttgtgact
    17701 agcttttaat gctttgccaa acattggtgc atctttaaat tcaggaattt caccttttgt
    17761 ttcgctacgg ggatattgaa gtagcattcc aggtgaataa ggcctaatta taaagttttt
    17821 taaataacct gaagaaggaa ccatatatcc atacatatag ttttcatagc catcacattc
    17881 atatatatgc acagtcggtt ctggacgata tttcaatatt tcgagtttat ctttatatcc
    17941 aagttcttca tataactttt ctgcttcttc tattgttatt ttagtgcgat taaatggtaa
    18001 gtccgcttca acaatattac gcatttcccg ttgcaatttt tcaacaagca atcgatcaac
    18061 agtgacgcct ttatttgtta tttgaatgaa gattgagcgc gacacattat atgaaaaacg
    18121 cacatttagt tcgggtctaa ttcttttcat tgccatcgct gctacaaagc gaagcgatgt
    18181 ttgataaatg cgaatacctt cgccattatc tagtgtcttt gtttcaagta tcgcatctcg
    18241 attcaaaaga aatgttaatt ctcggacgcg tccattgaca taagcacaaa ttaagttctt
    18301 atccggacct acaatatcta agatacgtac tggctcgtca tgagttgtag tttttccatt
    18361 gtaaactaca gtaaaagttt ttgttttttt attttccata gattctcctt ttatataaag
    18421 cttaaatagt aagttaatta tatacaacaa caagtttttt tcctaaagaa ataacaatag
    18481 caattattaa ttagctaaag tacgattcgt aattagatat gatcgtttca agttcggtgt
    18541 cgtcattagg taatgatatt tccttattac tactcccgta actcaaagta ggtgtatcta
    18601 aaggggtgtc ctcatttact aagtaattta cataaccttt cttttcaaag taaggaaaaa
    18661 actttgtatt aattaattcg tgatgagtag cgttattaaa attatatgta aatgtcacat
    18721 actttttaaa atcaggttta atgttgtctt ttttatctgt tgtgtaatag ttatttgtgc
    18781 gaacatgttc tttaaaaaat ttattaactt ccaattgtgt acttaaatta ctagcgccac
    18841 gttcgcgatg gaccatctca ccttctttat atatcaaaat attcggtgca cccatcacat
    18901 tagttgtttc aatataatat tcgcgattat cgccatattc ttgactataa aggtcaatat
    18961 aataaatttc gattttggtt ttttgcacgt aatttagtaa taatggttca atctctgcac
    19021 aatattgaca gcgatgacta ccaaaatata gcacgaaaga ttttttccct tttgtaaaag
    19081 cttcatgata tgcttttgtc atattatatg tataattttc acttgttata gtagtttcta
    19141 atcgttttgg tacgagtatt tgattagggt tagaaacttt acatccgctt aaaaatgagc
    19201 taaaaatgta aataaatagt aaaagttgta atatcttttc ttttttcata ttataattaa
    19261 accacaatca taattagaaa tcatttttca agattattac atttaaggag atattatgaa
    19321 tatttggcat gacatggacc cacgacgtat tacaactgaa cgattcgatg cagttgtaga
    19381 aattagcagg ggtcaacaat caaagtacga acttgataaa gaaacaggat taattagact
    19441 tgatcgcgtt ctttacacct caacccacta tccacaaaac tatggattta ttccgcggac
    19501 ttatgcagga gacgacgacc cattggatgt gctcatttta tgttcacaac ctttagtacc
    19561 gatgacaatt gtcacatgta cccctattgg tgcggttaga atgcttgatg gtggtgaact
    19621 tgacgaaaaa attattgcca ttgcggcaaa tgatccaact tattcttcgt atgcatcggt
    19681 tgatgaaatc ccgcaacatg tgttacaaga aattcgtcac tttttccaag tgtataaaac
    19741 acttgaaggt aaagaaacag tcgtacatca tattcaaggc cgtgataaag ctattgatat
    19801 aatagagaaa tctattgaaa tgtataacga agtatttaat ataaaacaag cggaagaata
    19861 atgctaaata ttattcttta tcaaccagag atgccggcta atgtcggcaa tataatgcgt
    19921 acggcagtgg caattgatgc tacgctacat attatagggc cgaccccaat taagcttgat
    19981 gataagagtt taaaacgtgc agggatggac tatgtccaag acttatcctt ttttatttat
    20041 gactcaatag acaaatttat ggaaaaacat cacgacaaaa atattttttt cgtgacgcgg
    20101 tatagtaaaa ataattatac agaagttgat tatggtcgtt ttgatgctgg tatatatgtg
    20161 atgtttggta atgaatcata tggtttacca atggaattat tacgcaagca taaagaaaac
    20221 accttgcgaa taccaatggt agcgactgca cgtagtttaa atcttagtaa tgctgtggca
    20281 gttgttagtt atgaaatatt acgccaacaa ggattctata accttgccac aagtgaagta
    20341 attaaaggtg atgattattt ataaaagcgc ttgatattgt tttcaaaaaa cggttttaaa
    20401 tcatcattaa agcgtaaaaa gaatgtatta tatttacggg ttagtacttg tccagggaac
    20461 gccccctttt taatattata aatcggtgaa tacataactt caccatcaat ttgttttgct
    20521 aaaaataagc ataacattcc agcaaactct aacacatctt ttgctggatt ttcttgccta
    20581 ataattatat gcgctccagg ccgattttga atatgcataa aagtatcatt tttcttagca
    20641 attttaaagg ttaaaaagtc gttttgttta ttattttttc caaataaaat atcaatatct
    20701 ttataagtta ttacatacgg atttaattta gaaattattt gccgtttatc acgtcttttt
    20761 aatagtccaa gttcttcata aatttggaat atttctccag cactctctag actttttaaa
    20821 tgattttcaa tactttccag atactttatc ttctctttag tttcagcaat ttttgacatc
    20881 aatatatttt cggatttttt aattttttta gcttttttga aaagaatctc agcgttaaaa
    20941 agtgcatcaa aacgcggatc aacttctata gtttcacctt caatttgcac aaaatagtac
    21001 gaaattgctg gtttatatgt taataacata tttgctttgt ggtataaatc cggaagttct
    21061 tttaactttt ctaagtcttg ctcataattc cttattagtc gttttgagcg tttgatattt
    21121 gtttctaaat atacataaac atcacgaaag ttttgctttt taattcgttc acgattctct
    21181 ttaagatatg cctgtaaata atctgtttta gcgttagaaa tttgcggcga aaaagcacct
    21241 ttttcaggta gtgaatattt actgccacca cgaataaatc gaccatcttt tgcgcgttct
    21301 tgatgatagc gatacgctgc taaaatacgt cctttattat tcactaatac agcttgggga
    21361 tgacgcggaa ttaattcaat aaaaagtgtg acttcttcaa gttccaaaac attgtttctt
    21421 tgctcgaaaa ctagtgataa aatcatatca tcattaggtt gattcatagc cactaagtta
    21481 ccacctaaaa actgtcttaa atttatggca aaactatgta attctccgcc ctcggggaaa
    21541 tcacttaaat cagcgttaaa cacaataaaa ggcgtcggac tatttaatga gacaaataag
    21601 ttattaccat gactaaattc aaatacaaaa tcaaccaaac ttaattgacg aattcgcact
    21661 agaaataaac cttcaaaatg tttattagcg ttaattaatt gttgtaaatc atttggaaaa
    21721 agtctttgca tgctcttatt ctacaacaag aatgtgacaa gtttgttatt tattaataaa
    21781 taagatacaa tttattaagg aaaatcaagg ggaaaatata tgagacaagg ctttattgtt
    21841 ttaataagtg gtccatcagg tgtaggcaaa ggaacactta ttcatagttt acttgccgat
    21901 caaacattga acttaagttt tagcgtttca atgactacaa gaaaaccacg tcctaatgaa
    21961 aaagatggga ttcattattt ttttgttagt catgaaacat ttttgaaaaa catcgaggaa
    22021 aacaattttt tagagcatgc tcagtttgtt ggaaatcgct atggaacgcc tcgcgactat
    22081 gttgaaaaat tattgaatga aggcaaaaat gttttaattg aaattgaagt tgatggtgcc
    22141 cgtcagttat taaaaaatgt tgatagtgaa aaagttcttt ccctttatat aatgccccct
    22201 tcagttcaag aattagtaag acgcttacgc ttacgtggct cggaagacga agcaacaata
    22261 cagcgtcgcg ctgaaagatt taaagaagaa attaaatata aagaccaata tcagcacatt
    22321 atcataaatg atgagttaga aacgtgtatt aatgatgtta aggcattaat tcgggagaag
    22381 atgaatattt aaatatgcaa ctcattgaag tttttacaca atattcaaaa ttaaatcgtc
    22441 cttttacata ttattatgat ggcgatgatt tagaagttgg agtaagagtt ctcatcgatt
    22501 ttaataatcg tgaaattgtt ggttatgtag cggccgtcaa aagcaccaaa gagacaattg
    22561 aaacggcaag taaacgccta ggctttgagc ttgctgaaat aacaaagatt ctcgatgaaa
    22621 aaccactctt aactaacgaa ttaatggaat tgagtgaaaa tgttactagt tattacttaa
    22681 caaatcggat tagtgtttta aatgcgatgt taccaccctc actacggcct agtaaaggta
    22741 gtttaagtgc gccaaagatt gcaatgcaaa aaaaagtaag ttttaatcct gaatttactg
    22801 attttgataa gttaacagat aaacaaagtt cattattaag tgaaataatg acgcatggta
    22861 gtgttttaca aacttcaaaa aatagaacgc aaataatgca gctattaaat aaaggtgctg
    22921 taattgtgga agagattgaa aaatacagat tacgctacga agattatccg cgtgaagatc
    22981 cacatgaatt aaccaatttg caaaaagaag cagtaaaatc cattttagaa gattataaag
    23041 agacattttt gctccatggt attaccggaa gtggtaaaac agaaatatat cttgcccttg
    23101 ccgctcacta tttagcacaa aataaacaag ttataatgct cgtaccagaa attgctttaa
    23161 ctggaacaat gcttgctaat ttttatcgaa gatttggtaa gaaaattgct atccttcata
    23221 gtgaacttac accagctgaa aaatacgatg aataccgtcg catcagcgct ggtgaagttg
    23281 atattgttgt tggtgccagg agtgctattt ttgccccatt acaaaatatt gggcttatta
    23341 tattagatga agagcatgct gaaacttata agcaagatgt tgcacctttt taccacgcct
    23401 taactgtagc gcaaatgcgg gcgaaaagct ttaacgctaa agtattagtt ggtagtgcaa
    23461 caccaagcat tgaaacgatg gcgcgcgcta gaaaagaagt ttatggttat tattacctag
    23521 gtgaaagaat taacttgcag cccttaccag aaacgacagt tgttaatatg ttagactata
    23581 aaaatgttac cactacttca ccatatattt ctaagttatt agcgaccgaa attagaaaag
    23641 ctattgatgc caagacacaa gtgattttat tagtaaaccg gcgggggtac gccacttttg
    23701 taagttgtcg tgaatgtggc gaatctaaaa agtgccctgc ctgtgcgata tcactcacct
    23761 atcataaagt aggaaataac ctaaaatgcc atcactgtgg cctttctttc cctaaaaatg
    23821 atccttgcat caaatgtggt agcactaatt tctcatatac tggatttgcc acacaaaaag
    23881 ctgaagaaga tattcaaaaa atgtttcctg atgcacggat cttgcggctt gatagtgatg
    23941 tgagtcgtaa agataaaaca ccacaagtag tgttaaacaa atttatgaac cacgaagcag
    24001 atattttaat tggtacgcaa atggtttcga aaggtcatga tttcccatta gttagtttag
    24061 tgggagtaat tggcactgat gctaatttat caataccctc atttagaagt agtgaacgaa
    24121 catttcagct aatcacccaa gcgattggta gatcaggtcg cgaaaaaata aaagggaagg
    24181 cgattattca aacaatgatg ccagaacatt atgttattca acatgcaaaa caacaagatt
    24241 attttggatt ttatgaagtt gaaattagac atcgttattt aacaagtaat ccgccttttt
    24301 attttttatt atcaattacg atgagccatc aaaacgaaag aacggtaaaa gatactatat
    24361 taacattaaa actaaatctt attaaagcac tgggtgaaga tgcgactgtg attggtccaa
    24421 ccgctacttt ttacccagca agtggtaaaa cctttaacga aacacttatt gtgcggtata
    24481 aaaattattt taaaattaaa cctgcacttt taactacatt aagtccattt agagaacaat
    24541 cttctcttta tgtacatata aatgttgatc catatgatgt atagaaagga aaaatattat
    24601 ggtcattatt aaaatttatt ctcaagacat ttatgtcgct gctgaaatca gtgttaaata
    24661 cgctgatgct attgctaaaa cactaggttt aacaaaagaa gatgtattct tactcggtag
    24721 tgatggtgct ttaatcgcgg aaggtgttga tcaagtaagt tggtttacat atttagaagt
    24781 tactattcct gcaaatttaa agcacaaaca agatgcatta agcaaaacgc ttttagaaat
    24841 attcaataat tatggtgtcc accttatgat taaatatgac tatgtaacaa aagataatat
    24901 tgttaaaatt ttaaaccctg attttaaagt ttttaatgaa acaatggttg aagttttgcc
    24961 tactggcaat tactacgatg aagatgatgt cgactgggat gatgattggg acgaagacga
    25021 agacaaagag gttagataac atacttatct ttacaaataa agattaaatt tttaaaaaca
    25081 aac
//
LOCUS       DAOSLP010000012        24398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_2288_length_24398_cov_10.470977, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000012 DAOSLP010000000
VERSION     DAOSLP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 24398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24398
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2288_length_24398_cov_10.470977"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..743)
                     /locus_tag="PK460_02015"
     CDS             complement(87..743)
                     /locus_tag="PK460_02015"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOE53906.1"
                     /translation="MNEIKTWEDFWEREKDSLYLQNLKVFLNAQDPLTTYPPRDKIFN
                     AFKLTPLEDVKVVIIGQDPYHQPGQAMGLAFSVPSNVPLPPSLRNIFIEMADDVGGPV
                     RTDGDLTYLAKQGVFLINTILTVERSKPLSHNIPEYKTLIVKVLEELNKRPQPIVFIL
                     WGSYARSFKKYINGPNKLVIESNHPSPLSANRGGFFGTRPFSRANNFLAKHGSNVINW
                     "
     gene            809..1897
                     /locus_tag="PK460_02020"
     CDS             809..1897
                     /locus_tag="PK460_02020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE53907.1"
                     /translation="MKILIVFNHPAPYKVALFNGLSAKHDVHVIFERFKASNRNQSFY
                     NESNYKFTLHKIKGVSLGDENHLSFGVVRHLKKHKYDLIIMNGYSTLTEMLALRYLKR
                     KKIPYAFYINGGFANEKECKLKKKIKTYFISGAEAYFSPAKKANEYLTYYGAPADRIY
                     NYPYSTVYEKDVLEVKTPTATKVLYWKEKGIEATNFTICITSFIKRKNNEALIKAWKE
                     IPKDYALILVGEGYEEPMYREIIANEKLTNVLLLPFAPKHLVFEYLSHSDNAVYISNY
                     DIYGHVINEALAHGLNVLTNTKMVAAIDLIRDGQNGLIAYPEDDLVSRILLLLATDFF
                     NEAIATAKENTIEESVRVHLDILEKICE"
     gene            1888..2988
                     /locus_tag="PK460_02025"
     CDS             1888..2988
                     /locus_tag="PK460_02025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE53908.1"
                     /translation="MRIMYLTTARHPHDYEMFLKKYKKAPNPSNQNFHNKLINILRSK
                     FPLGAFSTRPMEKNIFLTQSKSDYFYYPGYINTPVLKRLGIITAGLQYYEARTPKVIF
                     VDTLNVTLLELARRLKKRHNVKVIGIVTDNPINITGASKNYSQDVFKKSLVCDGFISL
                     TQGLLRLFNPKQKPAIIVPGFIEEAESFTNKKEKYAFFAGALYPKYGVDALIKTFRKL
                     NPPFKLVIAGHGPLADDLLENKHPNIEFLGQVCPPVAFRLAQRAYVNINPRPIDEQVD
                     LYSVPSKVIDYINSGSVTISTENTEIRNLVGDNIYFIKDAEPHTILEALNAINKNYNY
                     YKQRAAEAKKALVKALDNKVLINKIEDLINKI"
     gene            3031..4524
                     /locus_tag="PK460_02030"
     CDS             3031..4524
                     /locus_tag="PK460_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOE53909.1"
                     /translation="MQRKEMRAPVVFTLIRNVTIALLSFISFPFASQALGTAAMGVYN
                     WANTFVYYFLIISRLGIPLIAIRECAKVKDDKDKLSEKVQSFFVLQLITTALSFVLLS
                     TIMFIGKDTPMFKGDTWELIFLLSLNFLVGVFSFEWVYIALDRVFYMSVRSLATTILA
                     TLLIIVFVQNDHDLFIYAFINLIATFVTVTLNLLLLKREGVKLSFRRKVNLKHLTKSL
                     LFVFGITFLITAYNQNDSLLLGYLDDSFRTSGAYSVGIRGIEIVITIITSLSGIFVIR
                     ATSALKNDDMPAFHRVISYSTNITFFIGVPAVLFMIGLSPEIVGFIVKDSPYWDEAAI
                     NNAILAVVLLSSLMLTYSIHDGIYQQVLVPLKKEKIYFFTMLFVLILNIVASVLLALF
                     VFKDNPLFAVALVTLLSEFVVLIILFITAKEYVIRHIFSLNNLKIILSAGVSLGLVML
                     IKHFLPAMAYLATVGLCIFIGGIIYIVMLLLLKEDLVYAQVKGVKNG"
     gene            4517..5437
                     /locus_tag="PK460_02035"
     CDS             4517..5437
                     /locus_tag="PK460_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOE53910.1"
                     /translation="MANPNFKENKLKRLIAKSIQPLLRIFSKKTYVSWQYRYITGHKL
                     DWKNLKRYTEKLQYLRLYYYPRNASVVKAASRDGARERVKSKGLEKLLIPIVGIYDKV
                     EDIPFEALPKRFVVKATHACALNHICLDKDTLDVNALKNKMRRWLKMDYGKKTVEPHY
                     SKIKPRLIVEHYVGKSDSLPTEYKMHVFNGKARYLYVVSGRGKDIRYDNLYVDFTPFP
                     ESQFNHWSSSDEVPRKPKNYEELVKHAEMLASDLLFCRVDFFIVDDHIYFNEFTFTPA
                     KGTLIFDDDKADFIISEWFDISSAKKNKAA"
     gene            complement(5409..6389)
                     /locus_tag="PK460_02040"
     CDS             complement(5409..6389)
                     /locus_tag="PK460_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53911.1"
                     /translation="MRKNKWIALLISVLTLTGCKAVTETFEKGAFHTQDFSENYYTNI
                     PERYLTSNYEETVYEISDRININQPLDNAFDNAIRNDLVNQNLSLEEAIKLYGSAETK
                     AIKKEDFRNEIEYMEAIFNSLSSAQNDKASWINYAANNNLVKGKYSKAIQESFKRGIF
                     SKLTDGIIKCDGTGPKVRMQISEQGMGQTFEHELISYNRFVLSARGGTSVDYGSYGKA
                     RVTKAKVKINISFFIEKSTTNSASKHTVTFIVPEMHSDNEAILFTSIISFDLREVLGA
                     ETLKRVNGLMISYELLEHEFLKPGGVLDTSLNEEFALMLYEVMLPYSSWR"
     gene            complement(6376..7764)
                     /locus_tag="PK460_02045"
     CDS             complement(6376..7764)
                     /locus_tag="PK460_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53912.1"
                     /translation="MKDKIRESKLALISLFLALEVVLFIALDFGNIGPVFKALAFLLT
                     AILAPFFFEEMKVDLGQGLYVLLMPILFYALVTLVAPAYGSYPEMYSNEVFMQLPFIT
                     RLINLLGIVGLLLLGYFIGKSEVFATKHIFIVILAGLAAPVLISLFATLINYGFFHPL
                     VYKSLVTYYAGHEYEVSSQASLLYGFRIMVGDLKALASMAVIITSAGFGLLFVNEKKD
                     KFTIISLVVISAVGVLTLIFTASFAALIYLLPALIFALLLKFDLLKKLKNKVTLGVVI
                     ALVGLGAIIFLLTAYNIFNLQAVWQTNRVTRKLFFNGYMQRFYIIFKEAFGFRNLYGH
                     FAMTVEGYKVFPSGNFLFDAMWIDGLIGFVALLAFFVMFVIRLVKYFNKDKDERVLKV
                     VLISVLLTAFFRFMLRYPFKIFTYQEFSDINNFPLINSPLFLVTVFIAGYVFVLKEDK
                     KIEEEEVTHAEK"
     gene            complement(7764..8705)
                     /locus_tag="PK460_02050"
     CDS             complement(7764..8705)
                     /locus_tag="PK460_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53913.1"
                     /translation="MKRTRRSYIWEFLKSAIYVFRYKILRIKPPTNRIGKYKEITMQK
                     SNNAIEEAIRKGEPFAAIRFGAVEISALNNYEKIRLGFRRKYKKSVVYSIKNNAGVFP
                     PTQEIIDFYAKYLETNLKDTDYLGVSGVHMENYFFKHLTPDATPVQNWALDPLLGKWS
                     HLLAGKRVLVISPFAEQIEEQYEKRHLLFQAEPEILPEFTLVTIPAILTSGKAASKEF
                     DNWFEVLDYLKLEILKKDFDIALVGAGAYGTPLCLYIKSLGKMAIQSGGATQLLFGLM
                     GKRWEKREYVARHVNPHWSRPTVKPYGHKNIEGGCYW"
     gene            complement(8720..9406)
                     /locus_tag="PK460_02055"
     CDS             complement(8720..9406)
                     /locus_tag="PK460_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOE53914.1"
                     /translation="MENEKYKRLLQIISEEDLIKIQNKRIIIFGLGGVGGNALEALVR
                     SGFRRFTIVDNDVVDITNLNRQLIATTKTIGMPKVKAAKKRMLEIEPNCNINAVFEAV
                     TPQNVANFNLEFYDYIVDAIDSFEDKMALIIYALKHNLPIISAMGAGNKIDPTKVIIT
                     KIEKTEGCPLAKKVRYRLRQEKLKGLMVVTSKELPLTNAGNKPGSSAFVPPAFGLAMA
                     SYILRNVISK"
     gene            complement(9366..10343)
                     /locus_tag="PK460_02060"
     CDS             complement(9366..10343)
                     /locus_tag="PK460_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE53915.1"
                     /translation="MKWVFLGLAILIFLVISLYFWTVMITFRMLFRRPKLPKKLVFPK
                     AFFGGVFDKHIPEIEANHKKNAKKACEIVTISSSAGKLKGRFYENENSNKIVIFIHGY
                     YSSGMRDIGYFGSLHKELNVSLLVIDQRANGESDGVYTSFGALERYDIREWIFYLNAR
                     YKGEKDIYLHGISMGAATSLLVTALAALPPSFKGVIADCGFSRTNGVIFFAGKRMLKI
                     KPKLTFWGLNLIAKLAGGFDLNKVNVSRELHKNMKIPILFIHGTADLFVPYNMSVKNY
                     KATSGPKKLVAFPDAGHCESYLSNKALYTEEMRKFIYGKRKVQKTTTNN"
     gene            complement(10411..11619)
                     /locus_tag="PK460_02065"
     CDS             complement(10411..11619)
                     /locus_tag="PK460_02065"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOE53916.1"
                     /translation="MKKSLVYFQSGGPTAIINTSMYGVIKEAKRQTEYIDGIYGSLHG
                     IEGLLNDNLIDLRQEDEAQIELLKQTPAAALGTTRYKLSGDINHEDYQKILNNVKKHN
                     IGYMLVNGGNDSMDTCYKLSTFFRKMDFDCKVIGIPKTIDNDLAFTDHCLGYPSAARY
                     VVQSIQDIALDNKVYPKSKFVIVEVMGRHAGWLTAAASVIQDPELKPDYIYLPEEKFD
                     LEEFLARAKEVYERKKRALFVVSEGIETQMPNLDKSVDAFGHAQLGGFAAKLAGIVEE
                     RLCYSTRPIEFSLLQRAGTLAITETDQKEAIKVSTVAVKRIVAGKTGQMVIIDRKPGP
                     KYKVSYKLKNLRGIANVEHKMTPELIHDVNNGGKLFGEYLFPLIDKPIKLQLENGVAK
                     YTKLKKVKVK"
     gene            complement(11664..12872)
                     /gene="glyA"
                     /locus_tag="PK460_02070"
     CDS             complement(11664..12872)
                     /gene="glyA"
                     /locus_tag="PK460_02070"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006585103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOE53917.1"
                     /translation="MEKLFELVALETKRQNEGLELIASENFTSKKVRALAGSVLTNKY
                     AEGYPGRRYYGGCHYIDEIEGTAIELAKKLFNAKYANVQPHSGSSANMIAYASVLELG
                     DSILTLSLDEGGHLTHGSPVNFSSKHYKVTHYGLKDDGTIDYEALREIALKLKPKLIL
                     AGFSAYPFIIDFARIGKIAKEVGAYFMVDMAHIAGLIAGGYHPSPFPYADIVTSTTHK
                     TLRGPRGGIILTNSEEIIKKVNFTCFPGMQGGPLENIIGAKAQSFYEALQPEFKEYIK
                     RVVENTKVASETLRALGAEASETETHLFLLNTKKSYGITGKDAEALLETIKITTNKNM
                     LPKDDERPNTTSGLRLGFAAVTTRGLTKVNAAETARIIHEFLSKKITKEEAMIRVNKI
                     VDTLQAIDSL"
     gene            12969..13706
                     /locus_tag="PK460_02075"
     CDS             12969..13706
                     /locus_tag="PK460_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOE53918.1"
                     /translation="MRIKEHPIFKVPLAHRGLHNETIDENSMPAFEAAVKAGFGIELD
                     VHITADNKIVVIHDNTTTRTTGVEKVVNDTTYKDLKKLRLLHTKTEIPLLKDVLKMVK
                     GKVPVLVEVKAENKVPPALVTEVLKVIEKYPYKDKLAMQSFNPYVARDLKKGSTGIPV
                     GQLMSDVLYGQSKLVHFMYRSLLVLKISKPDFFNYEVKYIHKRNIQRKRKRLPLITWT
                     IDDYDKEKTAIALADNYIFEHIEIKER"
     gene            13708..15192
                     /locus_tag="PK460_02080"
     CDS             13708..15192
                     /locus_tag="PK460_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE53919.1"
                     /translation="MELKLNMKRTFLVGLAFMSIMLFWTMYDTLVAKMLVDNFGLNQT
                     FSGFIMALDNILALFLLPLFGRISDKTRTKLGKRTPFIIIGTLAASILFVSIAFFDLY
                     QQIAVTKHGFSLIESIGTAENPLYQFTLGTEVYTNNVRSALATERAQLIFQQVTKQQP
                     GLLIGFIVVLFFVLVAMGIYRSPAVSLMPDVTPKPLRSKANAVINFMGTMGGAFAYVL
                     VFLLASVPIFDKTGYVATFATTSLLMLLLIAIFVATVKENAWYKEMVEITEKYHLDSL
                     DIDDEGLDVSQHIDDEKMPKDVRRSFILILSAVVLWFMGYNAATTKFSVYAFNHLGLE
                     QFSLPPLIGIITAALAFIPIGYISQKIGRRRMILFGIVVLTIALGSAVFITGETKVLM
                     YIIMGLVGISWASINVNSYPMVVEMSRHGNIGVYTGYYYSASMSAMIITPILSGALMD
                     ITGWYPVLFIYSAVFMGLAFIPMYFVKHGEPAPVVLKEKAQKDE"
     gene            15358..15642
                     /locus_tag="PK460_02085"
     CDS             15358..15642
                     /locus_tag="PK460_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53920.1"
                     /translation="MKKKKTMVRLWGDHIVISSLFIGLAISLAFVALTFVTIEVLAKH
                     VAIIAEKKKDFIYGFGTLAILVAFVINNFWIKPQRNIVKSSDLNKKEREG"
     gene            15646..17037
                     /locus_tag="PK460_02090"
     CDS             15646..17037
                     /locus_tag="PK460_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOE53921.1"
                     /translation="MVITFSLILQMVGIALLMTALYLIIGIIPGTDETSVLVPMTLIL
                     VTLKLNPLVIMAGFIAAVVTLNIADSIPTAITSIPGGVMSTPLVGHSQTLKKHGLTSY
                     SVQWMMLGSLIGVLVSFPIALAFWGIFEFISVQTGLEISNFIKRYNEYIFLGGAILMS
                     LTSKEKVVSLLSIVPFGFATAFLRNPGLIINNTLLNTLIGGRTLSMTPFFLSITTGPL
                     VFALFELFFPSVFKNQKVEGKMTVILDDDVKKTKHGHKKIIAKTGIASALGSLLFFMS
                     PVGVTMMLGEFATKDEKDEVQQALQGVSAVNGLSSATYLAGIMISLFVFAFPISPAAL
                     GPGSVIFSPGGPIILEGNAGLAVPKGYLLIAIVIGLVIGLSLTVWLGMKYSHKMTELV
                     FKYISQETVLIFLFSIIALLSFAEAGLYGLIIVVSVGLLSGWLNKKGAGYGVQFMALY
                     AGGLFITILKALF"
     gene            17051..17941
                     /gene="mvk"
                     /locus_tag="PK460_02095"
     CDS             17051..17941
                     /gene="mvk"
                     /locus_tag="PK460_02095"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOE53922.1"
                     /translation="MDGYGLAKIILIGEHSVVYGHPAIAIPFYAMRSEVVLTPHEEFI
                     LDTKYYTGPLNNAVDELHGLVALITHMAEIYKKIKPVIISVRSSLPEKSGLGSSASVA
                     KAVIDAFNNHFHLELTHIDYFELLKISENIYHHNASGIDASTVIYEKPIIYQRPHIKP
                     LKFNIDGYLAVFYSNTNSATKSAVAHVSTHPERDIHIDALGSLTANAKWALENNDITL
                     LATMFNAAHTHLRALGISTPTLEELRTKLLDAGALGVKITGGGMGGCLIALFNDEEKA
                     LATKEKLKSFPSWMIDLRNM"
     gene            17941..18894
                     /gene="mvaD"
                     /locus_tag="PK460_02100"
     CDS             17941..18894
                     /gene="mvaD"
                     /locus_tag="PK460_02100"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOE53923.1"
                     /translation="MKYKKANVNIAILKYWGKVNALINIPYQTSISVTADNFYTLTNV
                     ELDPKATKDIVILDGTALSGISYTRVAHHLDALRKHFKRKEYCIVNSINRVFTKSGYA
                     SSASGFAALTAAYIDAIGAKVSKKEMSKLARLGSGSATRSIHGGFVIWHRGNSHDTSY
                     AEKLQVKWPEFRMLFTIIDPSVKKISSRVGMQITIEKAKSYADYVKQSNESVEPLITA
                     LKENDIDAVGRIAERAAELMRNVMLEAGLEYHTPKTQQLIQKVKAIRSKHKIPVYYTF
                     DAGPNLILFTLDEYVNEVIRLLPEVKISVSGVGGGITSARS"
     gene            18881..19858
                     /locus_tag="PK460_02105"
     CDS             18881..19858
                     /locus_tag="PK460_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53924.1"
                     /translation="MRVHEKVPGKLYVIGEYNVLKSGHGAIVAPVNLFLQGTFVANHK
                     TTIIEDGVAYPYQTLLENGCPHQLKNTVTTITFFNEYLALKNIPLQQFEYTIENNLIS
                     DEQVKFGLGSSAASVILTLKLLNRLYRTNLSPLVLFKMAVLIQRELNALTSGGDLACT
                     IFDMPLYYVRYDLNWLLNEKRTFELLEVAWPLLEIKLLTKLPTFLVGWTKETITPDTN
                     GTIDSAFFKKANKLVHHYLVTYDDIYIKQYQNLLDKLGLTRQGMITPRLEALVKTARS
                     LGVAAKISGAGYGDCGIAQINDQSVVEPLTNLWENEGIIVLDIWRKDNE"
     gene            19851..20828
                     /gene="fni"
                     /locus_tag="PK460_02110"
     CDS             19851..20828
                     /gene="fni"
                     /locus_tag="PK460_02110"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOE53925.1"
                     /translation="MSRRRKDEHIAIAKMAKIRKNDFSHIRFIHHSIPSLSLKDIDLS
                     TSFLGHKFNYPFYINAMTGGAKKGDKINAKLAILAQHFGLPLFVGSQSLAFKNKESRL
                     AFETLRFNFPDLFIVANINPNFTAEMASDAVEILGANALAIHVNSVQELVMGEGDRDF
                     TKWKDNIANILKTVKVPVIVKEVGYGMHEPTLLLLQELGVKHIDISGMGGINFSLIEL
                     KRQKKKDSPLLDFGISTVDALLLARNYPSFEVLASGGINNASSIIKSLAMGAKAAGMA
                     KYFLDLCNLPVPEMIKKVDELIIDLKKILVLLNVKSVHELNLDHLMFDL"
     gene            complement(20823..22184)
                     /locus_tag="PK460_02115"
     CDS             complement(20823..22184)
                     /locus_tag="PK460_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE53926.1"
                     /translation="MKVKLVAQLRFLLSLFELLFGELQEIPGSTLGDILVLLNTIVLS
                     FLSVLYFYQVLLSIFSVVVRAKKYPETDVFPTYTFVTCARNEETVIAQLITTIRALDY
                     PQEKINIHVVADNCTDNTAIIAAQLKAKVYTRNDLTKIGKSYALEYYFNEQKKVVPAE
                     DFAGYIVIDTDNVLDAQFLKEVNKVYQATNAKVVATYRASTNAGDSLWAFGTGYSFLR
                     ECCLMHKGREVLGLSSYVSGTGFFVSYEKMQEVEGWNHHLLIEDIEFSAAHVMEKGRT
                     HYAHNAIFYDEQAVNFKSSWHQRMRWVKGLFQVSKVYTWSLFKSIFARKRTLKERFSS
                     YEAFLFSTPFPAYIIWWFILYGIGAAINLAITQDINYFIQTYLFTLFDFLLGFYLFTT
                     LMSLLISVSNWKRIKMHPLKKIMLPFVAFFFLLSYVPMLLIAPFVKVTWRPVKHYGLK
                     KPL"
     gene            22288..22797
                     /locus_tag="PK460_02120"
     CDS             22288..22797
                     /locus_tag="PK460_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE53927.1"
                     /translation="MLKTNLLRAFSRRCFLLYISVFFLTSLGFGTDKLGLIAALSPFA
                     ALLGNIIFQRIAKNLEVNRILMFIFSIIETSIALIFFIFPKQPFIFYAIIITCVSTIN
                     GPFYSLLDGYSGTYISERNKQYSSMRVMGTLSYVIGPLIGGFLVDNMVLDFKHYFSCH
                     QCFTSLLPF"
     gene            22761..23492
                     /locus_tag="PK460_02125"
     CDS             22761..23492
                     /locus_tag="PK460_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53928.1"
                     /translation="MSSVFYIITSFLIYYLPKQKIELHIIVPPEEKKRLKNIGKPELI
                     AYLLFNFFVIGVTVVTDNFFGIYLRTELGVTTTQFGYIVAIAIAIEAGVMFLLALRKN
                     NSFRVPAFSFFFIGTFLLIRPLVIVLNLSPTLTMILSVFRGIAWGYYIVFNVRFLARV
                     IPLKHLTKALLTGSIATTSSRIITSLIVGESLKHHSYQTVFFFSMFSVVFGMTISIII
                     AEIDRRKQKKVDMIVPFIGSDKDLN"
     gene            complement(23489..23974)
                     /locus_tag="PK460_02130"
     CDS             complement(23489..23974)
                     /locus_tag="PK460_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOE53929.1"
                     /translation="MEIRDVTNVLFIKNNKVLLGYKKRGFGKKKYNGFGGKLKSGETI
                     EMAAIREAKEEAGLTMKTYYKAAEIDFAESYPLRMHLYVCTEWSGEITESDEMVPRWF
                     GFNEVPVDEMWDDDKYWLHLALSGQKFRASFVFENDDDVTGTAENKVISYSIKLVDTL
                     D"
     gene            complement(24111..24314)
                     /locus_tag="PK460_02135"
     CDS             complement(24111..24314)
                     /locus_tag="PK460_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53930.1"
                     /translation="MVKEFGLPWIAHLLLQFFIGPIWGAVIRLVRGRDLWAVIYLLTG
                     GFFVIGWIYDLVMLSIHRDYKLA"
BASE COUNT         7641 a         4333 c         4228 g         8196 t
ORIGIN      
        1 tcattgttcc tctcagccag gcacacctag ctcccgtgag ttcattatat caaatcgcaa
       61 actttgtagt gtttttttgt cttatcttac caattaatta catttgaccc gtgttttgca
      121 aggaaattat tagcacgcga gaaaggtcgt gttccaaaaa agccacctct attcgcggat
      181 aatggcgaag ggtgatttga ctcaatcact aatttattag gaccatttat atatttctta
      241 aatgaccttg cataactccc ccataagata aagacaatcg gttggggtct cttatttaat
      301 tcttcaagta ctttaacaat taatgtttta tattccggaa tgttatgtga aagtggttta
      361 cttctttcaa cagttaaaat ggtattaata agaaatacgc cttgtttagc taaataagtt
      421 aaatctccgt ccgtgcgaac tggtccaccc acatcatcag ccatttcaat aaatatattt
      481 cttaagcttg gcgggagcgg cacatttgaa ggcacactaa aagctagtcc cattgcttgg
      541 cctggttggt gataaggatc ttgcccaata atcacaactt tgacatcttc tagcggtgtt
      601 aatttgaaag cgttaaaaat tttatcgcgc ggtggatatg ttgttagggg gtcttgggca
      661 ttaagaaaaa cttttaaatt ttgtaaataa agtgagtctt tttcgcgttc ccaaaagtct
      721 tcccaagttt taatttcatt cataaaataa ttatatcatc attgctttgc aatgtttctt
      781 ggcttaagtt gttgtataat ttatacatat gaaaatatta attgtgttta atcatcctgc
      841 cccatataaa gtcgcactct ttaatggttt aagtgcaaaa catgatgtgc atgttatttt
      901 tgaacgcttt aaagcaagca atcgtaatca aagtttttac aatgaatcaa attataaatt
      961 taccctccat aaaataaagg gtgtctctct tggcgacgaa aatcatcttt cgtttggtgt
     1021 agtgcgtcac ttaaagaagc ataaatacga cttaattatt atgaatggtt actcaacatt
     1081 aacagaaatg cttgcacttc gttatttaaa gcgtaaaaaa attccttatg ctttttatat
     1141 taatggcggt ttcgctaatg agaaagaatg taagcttaaa aagaaaatta aaacttattt
     1201 tattagcggc gcagaagcct actttagtcc tgctaaaaag gcaaatgagt acttaactta
     1261 ttacggagcg cccgctgata ggatttataa ttatccttac tccacagttt atgaaaaaga
     1321 tgttttagaa gttaaaacac caaccgctac taaagttttg tattggaaag aaaaaggaat
     1381 tgaagcaact aattttacga tttgtataac tagttttatt aaacgtaaaa ataatgaagc
     1441 gcttattaaa gcgtggaaag aaataccaaa agactatgcc ctcattctag taggtgaagg
     1501 ctatgaagaa ccgatgtatc gcgaaattat tgcaaatgag aaattaacaa atgtacttct
     1561 gcttccgttt gcgcctaaac accttgtatt tgaatattta tcacatagtg ataatgctgt
     1621 ttatattagt aattacgata tttatggtca tgtcattaat gaagcactag cgcacgggct
     1681 taatgtttta acaaatacaa aaatggtggc cgcaattgac ttaattcgtg acggacaaaa
     1741 cggtttaatt gcttatccag aagacgattt agtaagcaga atattactac tgcttgcgac
     1801 tgacttcttt aatgaagcaa tcgctaccgc aaaagaaaac acaattgaag aatcagtgcg
     1861 agtgcactta gatatattgg agaaaatatg cgaataatgt atttaacaac tgctcgtcat
     1921 ccgcatgatt acgagatgtt tctaaaaaaa tataaaaaag ctcctaatcc aagtaatcaa
     1981 aactttcata ataaattaat taatatttta cggagtaaat ttccgcttgg tgcctttagt
     2041 acacgcccga tggaaaagaa tattttctta acgcaaagta aaagtgatta tttttattat
     2101 cctggttata ttaatactcc cgttcttaaa cggcttggca ttattactgc tggattacaa
     2161 tactatgaag cacgaacacc aaaagttata tttgttgata cattaaatgt gacgcttctt
     2221 gaattagcgc gtagattaaa gaaaagacat aatgttaaag ttattgggat cgttactgat
     2281 aacccaataa atattactgg tgcaagtaaa aactatagcc aagatgtatt taagaagagt
     2341 ctcgtgtgtg atggcttcat tagtttaact caaggactct tacgcctttt taatccgaaa
     2401 caaaaaccag caattattgt tccgggcttt attgaagagg ctgaaagttt caccaataaa
     2461 aaggaaaaat acgccttttt tgctggagca ttatacccaa aatacggagt tgatgcttta
     2521 attaaaacat tccgtaaact taatccgcct tttaaattag tcatagcggg acacggcccg
     2581 cttgcggatg atttacttga aaataagcat cctaatattg agttccttgg tcaagtttgt
     2641 ccgcctgttg cctttcgttt agcgcaaagg gcgtatgtca atattaatcc gcggccaata
     2701 gatgaacagg ttgatctcta ttcagtacca agtaaagtta ttgattacat caatagcgga
     2761 agtgttacta tttccactga aaacacggaa attagaaact tggtagggga taatatttac
     2821 tttataaaag acgctgaacc acacacaatt cttgaagcac ttaacgctat taacaaaaat
     2881 tataactatt ataaacaaag ggccgctgaa gctaaaaaag ctttagtaaa agcacttgat
     2941 aacaaagttc ttattaataa aattgaagat ttaattaata aaatctagta aatccgccat
     3001 tattcattaa aaatatcata taatttactt atgcaacgaa aagagatgcg cgcccctgtt
     3061 gtttttacac taattcgtaa tgtaacaatt gcgctccttt cttttatttc ttttcccttt
     3121 gcctcacaag cacttggtac tgcggcgatg ggggtatata actgggcaaa tacttttgta
     3181 tattactttt taattatctc gcggctcggg attccgctta ttgcgattag ggaatgcgca
     3241 aaggtgaaag atgataaaga taaactaagc gaaaaagttc agtctttctt tgtgctgcaa
     3301 ttaattacga cagcactttc tttcgtgctt ttatccacaa ttatgtttat tggtaaagat
     3361 acaccgatgt ttaaaggtga tacttgggag ttaatctttt tattatcact aaacttcctc
     3421 gtgggcgtct tctcctttga atgggtatat attgctctag atcgagtatt ttatatgtct
     3481 gttcgttctt tagcgacaac aattttagct acattactga ttattgtttt cgtacaaaat
     3541 gaccacgatt tatttattta tgcttttatt aacttaatcg ctacttttgt aactgtgact
     3601 ttaaaccttc tcttattaaa acgcgaaggt gtaaaacttt cctttagacg aaaggttaat
     3661 ttaaaacact taacaaagtc gcttctcttt gtatttggta ttactttctt aattaccgct
     3721 tataaccaaa atgattcttt actccttggt tacttagatg attcttttag aacatctggt
     3781 gcttattctg ttggaattcg cggtattgaa attgttatta ccattattac ttctttaagt
     3841 ggcatctttg tgatccgcgc tacttctgca cttaaaaatg atgatatgcc tgctttccat
     3901 cgtgttataa gttacagcac taatattacc ttttttattg gtgtacctgc ggtcttattt
     3961 atgattggct tatcaccgga aattgtcggc tttattgtta aagatagtcc ttattgggat
     4021 gaagcagcga ttaataacgc cattttagcg gttgttctgc tttcatcttt aatgctaact
     4081 tacagtattc atgacggtat atatcagcaa gtgcttgttc cgcttaaaaa agaaaaaata
     4141 tacttcttta ctatgttatt tgtgcttatt ttaaatatcg ttgcaagtgt attacttgcc
     4201 ttattcgtgt ttaaagataa tccgcttttt gcggttgcgc ttgttacact gctttccgaa
     4261 tttgtagtat taattatttt atttattacc gcaaaagaat atgttattcg tcatatattt
     4321 agtcttaata atctgaagat tattttatct gcaggtgtta gtcttggttt agtaatgtta
     4381 ataaaacact tcttaccagc aatggcttac ttagcgactg tggggctttg tatattcatc
     4441 ggcggaatca tttatatcgt tatgttacta ttattaaaag aagatttagt gtacgctcaa
     4501 gttaaaggag taaaaaatgg ctaatcctaa ttttaaagaa aataagttaa aacgcttgat
     4561 tgctaaaagt attcaaccgc ttttacgtat attttccaag aaaacatatg tgtcgtggca
     4621 gtatcgttat attactggtc ataaattaga ctggaaaaat ttaaagcgtt atactgaaaa
     4681 acttcaatac ttacggctct actactatcc gcgtaacgca agtgttgtta aagctgcttc
     4741 gcgtgacggg gcacgggaaa gagtaaaaag taaggggctt gaaaaacttt taattccaat
     4801 tgtggggatt tacgataaag ttgaagatat cccgtttgag gcattaccaa agcgctttgt
     4861 cgttaaagcg acgcacgctt gtgctttaaa tcatatttgt ttagataaag acacacttga
     4921 tgtaaacgca ctaaagaaca aaatgcgtag atggttaaaa atggattacg gtaaaaaaac
     4981 agtggaacca cattactcaa aaataaagcc gcgcttaata gtggagcatt atgttgggaa
     5041 aagtgatagt ttaccaactg aatataaaat gcatgtcttt aacggtaagg cccgctactt
     5101 atatgtcgtt agtggacgcg gtaaagatat acgttatgat aatttatatg tcgattttac
     5161 accttttcct gaatcgcaat ttaatcactg gtcaagtagt gatgaagtgc caaggaaacc
     5221 taaaaactat gaagagcttg taaagcatgc ggaaatgctc gctagtgatt tattattctg
     5281 tcgcgttgat ttttttattg ttgatgatca tatttacttt aatgaattta cttttacacc
     5341 tgcaaaagga accttaattt ttgatgatga taaggctgac tttatcatta gtgaatggtt
     5401 tgatatttct agcgccaaga agaataaggc agcataactt catagagcat taatgcgaac
     5461 tcttcattaa gtgaagtatc aagtactcca cctggtttca gaaattcatg ttctaataat
     5521 tcgtaactta tcattagtcc gtttactctt ttaagtgttt ccgcacctaa tacttcgcgg
     5581 agatcaaaac taataatact cgtaaataaa attgcttcat tatcactgtg catctcaggg
     5641 acaataaatg taacggtgtg tttagaagca ctgtttgtcg tgcttttttc aataaagaaa
     5701 ctaatattaa tttttacttt agctttagtg acgcgcgctt taccgtaact tccataatca
     5761 acacttgtac caccgcgagc gcttaacaca aaacggttat aactaataag ttcatgttca
     5821 aaagtttgac ccatgccttg ttcactaatt tgcatcctaa cttttggacc agtgccgtca
     5881 cacttaataa taccgtctgt taacttcgaa aaaattccgc gtttaaatga ttcttgaatt
     5941 gctttactat attttccttt aacaagatta ttattagcgg cgtaattaat ccagctcgcc
     6001 ttatcatttt gtgcactgct caaactatta aagattgctt ccatatattc aatttcatta
     6061 cggaagtctt ctttttttat cgctttagtt tcagcactac cgtataactt aattgcctct
     6121 tcaagtgata aattttggtt aactaagtca tttctaattg cattatcaaa agcattatca
     6181 agtggttgat taatatttat acgatcacta atttcataaa ctgtttcttc gtaattactt
     6241 gttaaataac gttctgggat atttgtataa taattttcac taaagtcttg agtgtgaaat
     6301 gcaccttttt caaaggtttc cgtaactgct ttacaacctg taagtgttaa gacactaata
     6361 aggagtgcta tccatttatt tttccgcatg agttacttcc tcctcttcta tttttttatc
     6421 ttcttttaag acaaaaacat accccgcaat aaagactgtc actaaaaata gtggtgaatt
     6481 aatcagcggg aaattattaa tgtcactaaa ttcttgataa gtaaagattt taaaaggata
     6541 acggagcata aagcggaaga atgctgtgag taacacgcta atgagtacaa cctttaatac
     6601 tctctcatcc ttatctttat tgaaatactt cactaaacga atcacaaaca tcacaaagaa
     6661 tgcaagtaga gcaacaaacc caattaaacc gtcaatccac attgcatcaa ataagaagtt
     6721 acctgaaggg aatactttat aaccttcaac tgtcatcgca aagtgtccat ataaattacg
     6781 gaatccaaat gcttctttga aaataatata gaagcgttgc atgtagccgt taaagaataa
     6841 tttacgcgta acgcgattag tttgccacac tgcttgtaag ttaaagatat tataagcagt
     6901 tagtaagaaa ataatagcgc caagtcccac tagggcaata acgacaccaa gcgtcacttt
     6961 gtttttaagt ttctttaaga ggtcaaactt aagtaacaaa gcaaaaataa gcgctggtaa
     7021 taaataaata agagccgcaa acgaggcggt gaatattaag gtaaggacac caacagcact
     7081 aataactacg agactaatga ttgtaaattt atctttcttt tcatttacaa acaataagcc
     7141 aaagcctgcg ctagtaataa taaccgccat cgatgccagt gcttttaagt caccaaccat
     7201 tattctaaag ccatacaata atgacgcttg actactaact tcatattcat gtcctgcgta
     7261 ataagttact aaacttttgt acactaacgg gtgaaaaaat ccgtaattaa taagtgtagc
     7321 aaaaagcgaa attaaaactg gtgctgctag tccggcaaga ataacaataa atatatgttt
     7381 tgtggcaaat acctcacttt tgccgataaa atagcccaat aataaaagtc caacaattcc
     7441 taacaaattt attaatcgcg taataaatgg tagttgcata aatacttcat ttgaatacat
     7501 ttcaggataa gaaccgtatg ctggtgcgac tagcgtcact aacgcataaa aaagaatcgg
     7561 cattaatagc acatataaac cttggcctaa atcaactttc atttcttcaa aaaagaaagg
     7621 cgcaagaatc gctgttaaca aaaacgctaa tgctttaaat accggaccaa tattaccaaa
     7681 atcaagcgca ataaaaagca ctacttctaa tgctagaaat aatgaaataa gcgccaattt
     7741 actttctcta attttatctt tcattaccag taacaccctc cttcaatgtt tttatgacca
     7801 tatggtttaa cagttggccg tgaccaatgt ggattaacgt gacgcgcaac atattcgcgc
     7861 ttttcccatc ttttacccat aagtccaaaa agtaattgtg ttgcaccgcc actttgaatt
     7921 gccattttgc ctaagctttt aatatataga caaagcggag tgccgtaagc ccccgccccg
     7981 acaagagcaa tatcaaaatc cttctttaaa atctctagtt ttaaataatc aagtacttca
     8041 aaccaattat caaattcttt acttgctgct ttaccagaag ttaaaatcgc tggaatcgtc
     8101 actagtgtaa attccggtaa tatttctggt tctgcttgaa aaagcaggtg tcttttttca
     8161 tattgttctt cgatttgttc ggcaaatggt gatataacaa gtacgcgttt gcctgctaaa
     8221 agatgtgacc atttaccaag aagcggatct agtgcccaat tttgcacggg agtagcatct
     8281 ggtgttaagt gtttaaagaa ataattttcc atatggacac cgctaactcc taaataatcc
     8341 gtatctttta agttagtctc aagatattta gcgtaaaaat caataatttc ttgcgtaggc
     8401 ggaaataccc cagcgttatt tttaatgctg tatacaacgg actttttata tttccgtctg
     8461 aaaccgagac ggattttctc ataattatta agtgcactta tttctacagc gccaaatcga
     8521 atcgccgcga aaggttcgcc tttccggatt gcttcttcaa tcgcgttgtt acttttttgc
     8581 atcgtgattt ctttatattt accgatgcga tttgttggtg gcttaattct taaaatttta
     8641 tagcgaaaaa catatattgc gctttttaag aattcccata tatatgagcg acgtgttctt
     8701 ttcatacatt tatcatacac tatttagata ttacatttct caaaatgtaa cttgccatcg
     8761 ctaatccaaa ggctggtggg acaaaggcac tacttcctgg tttattacca gcattagtta
     8821 atggtaattc ttttgaagta acaaccatta atcctttcag tttttcttga cgtaagcgat
     8881 aacgcacttt tttcgctagt ggacaccctt ctgttttttc tattttagta ataattactt
     8941 tagttggatc aatcttgttg cccgcaccca ttgcactaat aataggcaaa ttatgtttta
     9001 aagcataaat aataagtgcc atcttatctt caaaactatc aatagcgtca acaatataat
     9061 cataaaattc taaattaaaa ttagcgacat tttgcggagt aaccgcttca aaaacggcat
     9121 taatattgca atttggctct atttctaaca tacgcttctt tgccgctttt acttttggca
     9181 taccaatcgt ttttgttgta gcaattaatt gtcgattaag attagtaata tcaacgacat
     9241 cattatcaac aattgtaaag cgtctaaaac cggagcggac aagcgcttca agcgcattac
     9301 caccaacgcc gccaagtcca aaaataatga tgcgtttatt ttggatttta ataaggtctt
     9361 cttcactaat tatttgtagt agtcttttgt acttttcgtt ttccatatat aaactttctc
     9421 atttcttcgg tgtagagtgc tttatttgat aaatatgatt cacaatgacc tgcatcagga
     9481 aacgccacta gttttttggg accacttgtt gccttataat ttttaactga catattataa
     9541 ggaacaaata aatcagcggt accatgaata aataaaattg gtatcttcat gtttttatgt
     9601 aattcgcgac ttacattaac tttattaaga tcaaagccgc ccgctaattt agctattagg
     9661 ttgagtcccc aaaaagtaag tttcggttta atcttaagca tgcgcttacc ggcaaaaaag
     9721 attacaccat tagtgcgcga aaatccgcaa tcagcgataa cacctttaaa gctcggtggt
     9781 aatgccgcta gtgctgtaac gagtaagctt gtcgcagccc ccattgaaat tccgtgaaga
     9841 taaatatctt tttcgccttt ataacgagca tttaaataaa agatccattc cctaatatca
     9901 taacgctcaa gcgccccaaa tgatgtataa acaccatcac tctccccgtt agcgcgttgg
     9961 tcaatgacaa gtagtgacac atttaactct ttatgtaatg aaccaaaata acctatatca
    10021 cgcattccag aagaataata accgtgaata aaaataacta tcttattact attctcgttt
    10081 tcatagaatc gtccttttaa tttacccgca cttgaactaa tagttactat ttcacatgcc
    10141 tttttagcat ttttcttatg gttagcctct atttccggaa tatgtttatc aaaaacacca
    10201 ccaaaaaaag cttttgggaa cactagtttc ttaggaagtt ttggccgacg aaagagcatc
    10261 cggaatgtaa tcatgacagt ccaaaaatac agtgatatta cgagaaatat taagattgct
    10321 agccctaaaa atacccattt catacattta ttataacttt taataaagaa aaagcggagt
    10381 ttatactccg ctcactattt tttttactaa ttatttaact ttaacttttt taagtttagt
    10441 gtatttagcg acaccatttt ctaattgaag tttgattggt ttatcgatta gtgggaataa
    10501 atattcacca aataacttac cgccattatt aacatcgtga atgagttctg gtgtcatttt
    10561 atgttcaaca ttagcaatac cgcgtaagtt tttaagttta taacttactt tatattttgg
    10621 tcctggttta cggtcgataa taaccatttg tcctgtttta ccagcaacaa tacgtttgac
    10681 cgcaacagtt gaaactttaa ttgcttcttt ttggtctgtt tcggtaattg ctaaagtacc
    10741 tgctctttgt aaaagactaa attcaattgg gcgcgttgaa tagcataagc gttcttcaac
    10801 gatacctgct aatttggcag caaagccacc aagttgagca tgaccaaatg cgtcgactga
    10861 tttatcaagg ttaggcattt gtgtttcaat accttcacta acgacaaata atgcccgttt
    10921 tttgcgttca taaacttctt tagcgcgcgc taagaattct tctaagtcaa atttttcttc
    10981 aggtaaataa atatagtctg gtttaagttc tggatcttgg ataacacttg cggccgcagt
    11041 taaccatcca gcgtggcgcc ccatcacttc gacgataacg aattttgatt ttggatacac
    11101 tttattatct aaagcgatgt cttgaattga ttggacaaca taacgggctg cacttggata
    11161 acctaagcag tgatcagtaa acgctaagtc gttgtcaatt gtctttggaa taccgattac
    11221 tttacaatcg aagtccattt tacggaagaa agttgataat ttgtaacaag tatccattga
    11281 atcgttacca ccattaacta acatgtaacc aatgttatgt tttttaacgt tgtttaatat
    11341 tttttggtaa tcttcatgat taatatcacc agaaagttta tagcgcgttg tccctaacgc
    11401 cgcagcaggg gtttgtttta ataattcaat ttgcgcttca tcttcttgtc gtaaatcaat
    11461 aagattgtcg tttaataaac cttcaatacc gtgtaaacta ccataaattc catcgatata
    11521 ttcggtttgc cgtttggctt ctttgatgac accatacatt gaggtgttaa tgattgcggt
    11581 tggtccgcct gattggaaat acactaatga ttttttcata tttttactcc ttttctcacc
    11641 cgagattata ccatgagtga ctattaaaga ctatcgatag cctgtaaagt atcaacaatt
    11701 ttattaactc taatcatcgc ttcttctttc gtaatcttct tacttaagaa ttcatgaatg
    11761 attcttgcgg tttcggcagc attaacctta gttaatccgc gtgttgttac cgccgcaaag
    11821 cctaggcgaa gaccagatgt cgtatttggt ctttcatcat ccttaggtaa catattttta
    11881 ttcgttgtaa ttttaattgt ttcaagtagt gcttcagcgt cttttccagt aataccatac
    11941 gactttttag tgttaagtaa aaagaggtgt gtttctgttt cactcgcttc ggcaccaagg
    12001 gcacgaagtg tttcactagc gactttagtg ttttctacaa cacgcttaat atattcttta
    12061 aattcgggtt gcagcgcttc ataaaatgat tgcgcctttg cgccaataat attttcaagt
    12121 ggtccgcctt gcataccagg gaaacaagta aaattaactt ttttaataat ttcttcacta
    12181 ttggtaagga taataccacc gcgcggtcct cttaatgttt tatgggttgt acttgttact
    12241 atatcagcgt aagggaaagg actagggtga tagccaccag cgattaatcc tgcaatatga
    12301 gccatatcaa ccataaaata cgccccaact tctttagcaa ttttaccaat acgcgcaaaa
    12361 tcaataataa aaggataagc agaaaatccc gcaagaataa gttttggttt aagttttaaa
    12421 gcaatttcgc gtaatgcttc gtaatcaatt gtgccgtcat cctttaaacc gtaatgcgtc
    12481 actttataat gttttgatga gaagttaaca gggctcccgt gtgttaagtg cccgccttca
    12541 tcaagtgaga gggttaagat gctatcacct aactctaata cactagcgta agcaatcata
    12601 tttgcactac taccactgtg tggttgtaca tttgcgtact tagcgttaaa tagttttttt
    12661 gctaattcaa tagcggtgcc ttcaatttca tcaatataat gacatccgcc gtaataccta
    12721 cgccctggat aaccttcagc gtacttattt gttaatactg atcccgctaa tgcacgaact
    12781 tttttacttg taaagttttc actagcgatt aattccaatc cctcattttg tctttttgtt
    12841 tctagcgcta ctaattcaaa taatttttcc ataacttaga ccatccttac agtattagat
    12901 tatagactaa tctagtctaa aaagtgctgt taattgactt tttatggtat tttcgtgaaa
    12961 ataaatacat gcgtattaaa gaacatccaa tattcaaagt cccacttgca catcgcggac
    13021 ttcataatga gacaattgac gaaaactcga tgcctgcttt tgaggcggcg gtaaaagctg
    13081 gttttggcat tgaacttgat gtgcatatta ccgctgataa taagattgtt gtcattcacg
    13141 acaatacgac tactagaaca acgggagtag aaaaagtagt aaatgacact acttacaaag
    13201 atttaaagaa attaagacta ctacatacaa agactgaaat accattatta aaagatgttt
    13261 taaagatggt taaaggtaaa gttcctgttt tagtggaagt taaagccgaa aataaagtcc
    13321 cacctgcact tgtcaccgaa gtgttaaaag ttatcgaaaa gtatccgtat aaagataaac
    13381 tagcgatgca gtctttcaac ccatatgttg cgcgtgactt aaaaaagggg agcaccggta
    13441 ttcctgttgg gcaactaatg agtgatgtac tgtacggtca atcaaaatta gtgcacttta
    13501 tgtatcgttc acttctagtc cttaaaatat ctaaaccaga cttcttcaat tatgaagtaa
    13561 aatatattca taagcgaaac attcagagaa aacgcaaacg cttaccactt ataacatgga
    13621 caattgatga ctacgataaa gaaaaaacag ctattgccct agctgataac tatatttttg
    13681 aacatattga aataaaggag agataatatg gaactaaaac ttaacatgaa gcgcacattc
    13741 ctcgtcggat tagcgtttat gtcaattatg ctgttctgga cgatgtatga cacactcgtg
    13801 gccaaaatgc ttgttgacaa ctttgggctc aatcaaacct tctcagggtt tattatggca
    13861 cttgataaca tcttagcgtt attcttgcta ccactatttg ggcgaatcag cgacaaaacg
    13921 cggaccaaat taggcaaaag aacaccgttt attattatcg gtactttagc cgcttctatt
    13981 ctttttgtta gcattgcctt ctttgattta tatcaacaaa ttgccgttac taaacacggt
    14041 ttctcattaa ttgaatcaat tggcactgct gaaaatccgt tataccaatt cacacttgga
    14101 actgaagtat atacaaacaa tgtacgttct gctcttgcaa ccgagcgcgc acaattaatt
    14161 ttccaacaag taacaaaaca acaacctggt ttacttattg gctttattgt tgttttattc
    14221 tttgtcttag tggcaatggg aatttaccgc agtcctgctg ttagcttaat gccagacgtt
    14281 acacctaaac cactccgtag taaagctaat gctgtcatta actttatggg gacaatgggt
    14341 ggtgcgtttg cctatgtcct agtattctta cttgctagcg ttcctatctt tgataaaacg
    14401 ggctatgtcg ctacttttgc aacaactagt ttactaatgc ttttattaat tgctatcttt
    14461 gttgcgactg ttaaagaaaa cgcatggtat aaagaaatgg tcgaaatcac tgaaaagtat
    14521 caccttgatt cattagatat tgatgacgaa ggtttagatg tatcacaaca tattgatgac
    14581 gaaaaaatgc caaaagatgt acgccgtagt tttattctca tcctaagtgc tgttgtatta
    14641 tggtttatgg gttataacgc tgcgactaca aagttttctg tttatgcctt taaccattta
    14701 ggacttgaac aatttagttt accaccactt attggcatta ttaccgctgc gttagcgttt
    14761 attccaattg gttacatttc acaaaaaatt ggtcgtcgcc gtatgattct cttcggtatt
    14821 gttgtgttaa caattgcttt aggtagtgcc gtctttatta ctggtgaaac taaagtatta
    14881 atgtacatta tcatgggtct tgtcggcatt agttgggctt caattaatgt taatagttat
    14941 ccgatggttg tggaaatgtc gcgtcacggt aatatcggtg tttacactgg ttactactat
    15001 agcgctagta tgagtgcaat gattattaca ccgattcttt ctggggcatt aatggatatt
    15061 accggttggt acccagtact ctttatttat agtgcggtct ttatgggcct agcgttcatt
    15121 ccaatgtact ttgttaagca cggtgaacct gcgccagtag tgttaaaaga aaaagcacaa
    15181 aaagacgaat aattcgctaa atacactatc gaaagatgac cctagtgggg tcatcttttt
    15241 tattattatc attattactt ttcaattatt tcttactgaa taattgattt ttgcttatgt
    15301 gttttgggat taattactta aaatgctata atgatctttc aagcgaggtt tgatgttatg
    15361 aaaaagaaga aaacgatggt gcgcttgtgg ggagatcaca ttgtaatatc atcactgttt
    15421 atagggcttg ctattagcct agccttcgtg gcattaacat ttgttactat tgaagtatta
    15481 gcaaaacatg ttgcgatcat tgccgaaaaa aagaaagatt ttatttatgg gtttgggaca
    15541 ttagcgattc ttgttgcttt tgtcattaat aatttttgga ttaagcccca acgcaatatt
    15601 gttaaatcaa gtgatttaaa caagaaagaa agagagggct aatttatggt tattactttc
    15661 tctttaattt tacaaatggt cgggattgct ctccttatga ccgctttata tctgattatc
    15721 ggtattatcc ctggcactga tgaaacttca gtcttagtgc caatgacact tattctagtt
    15781 actttaaaac ttaacccact tgtgattatg gctggcttta ttgctgcagt tgttactctt
    15841 aatattgctg actcgatacc gacagcaata acgtcgatcc caggcggggt aatgtcaaca
    15901 ccgcttgttg gtcatagtca aacactaaag aaacacggac ttacttctta tagtgtacag
    15961 tggatgatgc ttggttcttt aatcggtgtc ttagttagtt tccccattgc cctagcgttt
    16021 tggggtattt ttgaatttat ttctgtacaa actggattag aaataagtaa ctttattaag
    16081 cgttacaatg aatacatctt ccttggtggc gctattttga tgagtttaac aagtaaggaa
    16141 aaagttgttt cacttttatc aatcgttccg tttggatttg ccactgcttt cttacgtaat
    16201 ccaggtctta ttattaataa tacgctctta aatacgctta tcggtggccg gactttatca
    16261 atgacacctt tctttttaag tattacgact ggtccacttg tgtttgcgtt atttgaactt
    16321 ttcttcccca gtgtatttaa aaaccaaaag gttgaaggaa agatgactgt tattcttgat
    16381 gatgatgtaa agaaaacaaa gcatggtcat aaaaagatca ttgctaaaac tggaatcgct
    16441 agtgcgcttg gtagtttgct cttcttcatg tcaccggttg gtgtcacaat gatgcttggt
    16501 gaatttgcga cgaaagatga aaaagacgaa gtacaacaag ctttacaagg tgtttctgct
    16561 gttaacggtt tatcaagcgc tacttattta gcaggaatta tgatttcact tttcgtcttc
    16621 gctttcccta tttcaccagc ggcacttggt ccaggctccg ttatttttag tcctggcggt
    16681 ccaattattc ttgaaggtaa tgctgggtta gcggtaccaa aaggttattt attaatcgcc
    16741 attgttattg gtttagtaat tggacttagc ttaacagtgt ggcttggtat gaaatattct
    16801 cataaaatga ctgaacttgt atttaagtat atttcacaag aaacagtgct tatctttttg
    16861 ttctcaatta tcgcgcttct ttcttttgcc gaagctggtt tatatggttt aataattgta
    16921 gttagtgttg gccttctaag tggttggcta aataaaaaag gagcgggtta cggtgtacag
    16981 tttatggcac tttacgcagg tgggttattt attacaatcc tcaaagcgct cttctaatta
    17041 gggactaatt atggatggat acggtttagc aaaaattata ttaattggtg agcattccgt
    17101 cgtttacggt catcccgcta ttgcgattcc tttttatgca atgcgaagcg aagttgtttt
    17161 aactccgcac gaagagttta ttcttgatac gaagtattac actggcccac ttaataatgc
    17221 cgtagatgaa ttacacggac ttgttgcttt aataacacat atggctgaaa tttataaaaa
    17281 gattaagcct gtcattataa gtgttcgttc aagtttacca gagaaaagcg gcttaggctc
    17341 aagcgctagt gttgctaaag cggtaattga tgcttttaac aatcattttc atcttgaatt
    17401 aacacatatt gattactttg aattattaaa aatctctgaa aatatatatc atcataacgc
    17461 aagcggaatt gacgcttcta ctgttattta tgaaaagcca attatatatc aaagaccgca
    17521 cataaagcca ctgaaattta atattgatgg ctatttagcg gtgttttatt caaatacaaa
    17581 cagtgcgaca aaaagtgctg tcgcacatgt aagcactcat cctgaacgcg acattcatat
    17641 tgatgcttta ggttcattaa ctgcaaacgc taaatgggca ttagaaaata atgacattac
    17701 gcttcttgca acgatgttta acgctgctca cacacattta agagcgctag gtatttcaac
    17761 gcctacactt gaagaattac gcactaagct attagacgca ggtgcgcttg gtgttaaaat
    17821 tacgggtggg ggaatgggcg gatgtttaat tgccctattt aatgatgaag aaaaagcact
    17881 tgccactaaa gaaaaattaa agagctttcc atcatggatg attgatttga ggaacatgtg
    17941 atgaaatata aaaaagcaaa tgtaaatatc gctattctta aatattgggg gaaagttaat
    18001 gctttaatta atattcctta tcaaacaagt atttccgtta ccgcggataa tttttatact
    18061 ctcactaatg ttgaattaga tccaaaagcc actaaagata ttgttattct tgatggtaca
    18121 gcattaagcg gtatttctta tacaagagta gcccatcatt tagacgcact ccgtaaacat
    18181 tttaaacgta aagagtattg tattgttaat tcaatcaata gagtttttac taaatcaggc
    18241 tatgcttcaa gtgcgagcgg ttttgccgcg cttactgctg cttatattga tgcaatcggt
    18301 gcgaaagtaa gtaaaaaaga aatgtcgaaa ttagctaggc ttggttcagg ctccgcgact
    18361 cgttcaattc acggcggatt tgttatttgg catcgcggta atagtcatga cacaagttat
    18421 gctgaaaagt tacaagttaa atggccagaa tttagaatgc tttttactat tattgatccg
    18481 agtgttaaga aaatatcaag tagagttgga atgcaaatca ccattgaaaa agctaagtct
    18541 tacgctgatt atgtcaagca atcaaatgaa tctgtcgaac cgcttattac tgcgcttaaa
    18601 gaaaatgata ttgatgctgt tggtcgcatt gcggaacgcg ctgctgaact tatgcgtaat
    18661 gtaatgcttg aagcggggct tgaatatcat acaccaaaaa cacaacaatt aattcaaaaa
    18721 gttaaagcga ttcgctctaa acataaaatt cctgtttatt atacgtttga cgctggtcca
    18781 aacttaattc tttttacact tgatgaatat gttaatgaag ttattaggct tttaccagaa
    18841 gttaaaattt cagtaagtgg tgttggaggc ggtattacta gtgcgcgttc atgaaaaagt
    18901 accaggtaaa ctttatgtta tcggggaata caatgtttta aaaagcggac atggtgcaat
    18961 tgtagcgccc gttaatcttt ttttacaagg aacatttgtc gctaatcata agacaacaat
    19021 tattgaagat ggtgtcgctt atccctatca aacgttacta gaaaatggct gtcctcatca
    19081 attaaaaaat actgttacta cgattacatt ttttaatgaa tatcttgctt taaaaaatat
    19141 tcctttacag caatttgaat atactattga aaataattta attagtgatg aacaagttaa
    19201 gtttggttta ggatcaagcg cagcgagtgt tatcctaaca cttaagttat taaatcgttt
    19261 atatcgcact aatttgtcac cacttgtgct ttttaaaatg gcagtcttaa tccagcgtga
    19321 actaaatgcg cttacttctg gtggcgactt agcgtgtact atttttgata tgccgcttta
    19381 ttatgttcgt tatgatttaa attggttact taatgaaaaa cgaacatttg aattacttga
    19441 agttgcgtgg ccgcttttag aaattaaatt attaacaaaa cttcctacct tccttgtggg
    19501 ttggacaaaa gaaacaataa caccagacac taacggcact attgattccg ctttctttaa
    19561 aaaagcaaat aagcttgttc accattattt agttacttac gatgatattt atataaaaca
    19621 atatcaaaac ctacttgata aattaggatt aacgcgacaa ggaatgatta ctccgcgttt
    19681 agaagcatta gtgaaaactg cccgctcttt aggagtggcc gctaaaatat caggggcagg
    19741 atatggtgat tgcgggattg cccaaattaa tgaccagagt gttgttgaac cattaacaaa
    19801 tttatgggaa aatgaaggta tcatcgtact tgatatttgg aggaaagata atgagtagac
    19861 gccgtaaaga tgaacatatt gcgattgcaa aaatggctaa gataagaaaa aatgatttta
    19921 gtcacattcg ttttatccat catagtattc cttctttatc attaaaagat atcgatcttt
    19981 cgacttcatt tttaggacat aaatttaatt atccttttta cattaatgca atgacaggcg
    20041 gcgctaaaaa aggtgataaa ataaatgcta aattggcgat attagcccag cattttggcc
    20101 ttcctttgtt tgttggctca caaagtttag cgtttaaaaa caaagaatca cgacttgcat
    20161 ttgaaacact tagatttaac ttccctgatc tatttattgt cgcaaatatt aaccccaatt
    20221 tcactgcaga aatggctagt gatgcagtgg aaatcttggg cgctaatgcg ttagcgattc
    20281 atgttaatag tgtccaagaa ttagtgatgg gtgaagggga tcgtgatttt acaaaatgga
    20341 aagataatat tgctaacatt ttaaaaaccg ttaaagtacc ggtaattgtt aaggaagtag
    20401 gatacggaat gcatgaaccg actctcttac tcctccaaga acttggtgtt aagcatattg
    20461 atataagcgg aatgggtggt attaatttct ccttgattga attaaaaaga caaaagaaaa
    20521 aagattcacc acttcttgat tttggtattt ccactgttga tgcattactc cttgcccgta
    20581 attatccaag ctttgaagtg cttgcaagtg gcggtattaa taacgctagt tcgattatta
    20641 aatcactagc gatgggtgct aaagcggcag gtatggctaa atatttcctc gatttatgta
    20701 acttaccagt tcctgaaatg attaaaaaag ttgatgaatt aattatcgac ttaaagaaaa
    20761 ttttagtgtt attaaatgtt aagagtgttc atgaattaaa tcttgatcac ttgatgtttg
    20821 atttataatg gtttctttaa accataatgc ttaactggac gccaggtaac tttcacgaat
    20881 ggcgcaatta gtaacattgg tacatatgaa agcaagaaaa agaatgctac aaacggtagc
    20941 attatttttt taagtggatg cattttaatc cgtttccaat ttgaaacact aattaataac
    21001 gacattagtg tagtaaaaag ataaaaccct aataagaaat caaataatgt aaaaagataa
    21061 gtttgtataa aataattaat gtcttgagtg atcgctaagt taatagcagc acctatccca
    21121 tataatataa accaccaaat gatgtaagct ggaaacggag tcgaaaaaag aaacgcttca
    21181 taagaagaaa aacgctcttt tagtgtcctt ttgcgggcaa aaatggactt aaataatgac
    21241 caagtgtaaa cttttgaaac ttgaaataac ccttttaccc aacgcatccg ttgatgccaa
    21301 cttgatttaa agttaacagc ttgttcgtca tagaaaatcg cgttatgtgc ataatgagtc
    21361 cggccttttt ccatgacatg cgctgctgaa aactcaatgt cttcaattaa taaatgatgg
    21421 ttccagcctt caacttcttg cattttttcg taactaacaa aaaaacctgt gcctgataca
    21481 taagaactaa gacctaacac ctcacgacct ttatgcatta agcaacattc acgtaaaaaa
    21541 gagtaccccg ttccaaatgc ccaaagtgaa tcaccagcgt ttgtactagc gcgataagtc
    21601 gcgacaactt tagcgtttgt tgcttgatag actttattca cttcttttag aaattgcgca
    21661 tcaagcacat tatcggtatc aatcacaatg tagccggcaa agtcttctgc gggaactact
    21721 tttttttgct cattgaaata atattcaagt gcatagcttt taccaatttt tgttaaatcg
    21781 ttccgtgtat aaactttcgc ttttaactgc gcagcaataa ttgcggtatt atcagtacaa
    21841 ttatcggcga caacatggat gtttattttt tcttggggat aatcaagcgc tctaattgtt
    21901 gtgataagtt gcgcaatcac tgtttcttca ttccgcgcac atgtcacgaa tgtatatgta
    21961 ggaaatacat cggtttctgg atattttttg gcgcgaacaa caacagaaaa gatacttagt
    22021 agcacttgat agaaatataa aacactaaga aaggataaga caattgtgtt taataaaact
    22081 aatatatctc caagtgttga gcccggaatt tcttgtaatt ccccaaataa tagttcgaaa
    22141 aggctaagaa gaaaacgcaa ctgcgcaact aattttactt tcataaaatt attatatctc
    22201 ttttggggta aatactatcg aaaaatgata acatttagtt gatattatga aagcaatgag
    22261 aagactcgct aaagagcgca cgtatgaata cttaagacta acttacttcg tgcgttttct
    22321 cggcgatgct ttcttttata catttctgta ttttttttaa catctcttgg ctttggcact
    22381 gataaacttg gacttattgc tgctctttct ccatttgccg cccttcttgg taatattatt
    22441 tttcaacgaa tagcgaaaaa tttagaagtt aatcgtattt taatgtttat cttttcaatc
    22501 attgaaacga gcattgcgct tatcttcttt atttttccaa agcaaccctt tatattttat
    22561 gcaattatca ttacatgtgt aagtacgatt aatggtcctt tttattcact gcttgacggt
    22621 tatagcggaa catatatttc agagcgtaat aaacaatact cttctatgcg ggtaatgggt
    22681 actttatcat atgtcatcgg tccacttatt ggcgggtttt tagttgataa catggtgctg
    22741 gatttcaaac attatttctc atgtcatcag tgttttacat cattacttcc tttctaattt
    22801 actatttacc aaagcaaaaa attgaattac atattattgt tccgcctgaa gaaaaaaaga
    22861 gacttaaaaa tattggaaaa cccgaactaa ttgcttattt actctttaac ttcttcgtca
    22921 ttggggttac tgttgttacc gataactttt ttggtattta tttaaggact gaattaggag
    22981 ttacgacaac acagtttgga tatattgtgg cgattgcaat agcaattgaa gcaggcgtga
    23041 tgtttttact tgcactacga aaaaataata gtttccgagt gccagccttt tcgtttttct
    23101 ttatcggaac atttttgctt attcgtccgt tagtgattgt tcttaattta tcgccgacat
    23161 tgacaatgat attgtcagtc tttcgcggga ttgcatgggg atattacatc gtttttaatg
    23221 tgcgcttttt ggctcgtgtt atccccctaa agcacttaac aaaggcactt cttactggat
    23281 caattgccac aacttcaagt cgcattatta ctagtttaat cgttggcgaa agtcttaaac
    23341 atcattctta tcaaacggtg tttttctttt caatgttctc tgtagttttt gggatgacaa
    23401 ttagtattat tattgccgaa attgatcggc gaaaacaaaa aaaggtcgat atgatcgtac
    23461 cttttattgg ttcagataaa gatttaaatt aatctagggt atccactaat ttgatggagt
    23521 aactgatgac tttgttctcg gcagtaccgg taacatcgtc atcattttca aaaacaaaac
    23581 tggcgcggaa cttttgaccg ctaagtgcta gatgcaacca atatttatca tcatcccaca
    23641 tttcatcaac tggcacttca ttaaaaccaa accatcttgg taccatttca tcagattcgg
    23701 tgatttcacc gctccattca gtacatacat ataaatgcat ccgtaatgga tagctttccg
    23761 caaaatcaat ttcagcggct ttataatatg ttttcattgt aagacccgct tcttctttag
    23821 cttctctaat cgcagccatt tcaattgttt caccactctt taatttacca ccaaagccgt
    23881 tatatttctt tttaccaaaa ccgcgttttt tataaccaag taaaaccttg ttgtttttta
    23941 taaataatac gtttgtaaca tctctaattt ccatattttt ttgtctcttt tcaataatag
    24001 tgtgttaagc acattgctta atatacgcta ataaggggga aataacaaga aaaaggaata
    24061 aaaaaaacac accagaggtg tgttccttta acttcactaa ttttaattaa ttaggcaagt
    24121 ttgtagtctc tgtggatgct aagcatcact aagtcgtaaa tccagccaat aacgaaaaat
    24181 ccgccagtga gtagataaat gacggcccaa aggtcacgtc cgcgaactaa gcgaataact
    24241 gctccccaaa taggtccaat gaaaaattgg aggagtaagt gagcaatcca tggtaatcca
    24301 aattctttaa ccattttgta cctcctatgg tttatgattt tatgatatat caattaatat
    24361 aaaatttcaa tctaataaat gtaaataata gaagaaaa
//
LOCUS       DAOSLP010000013        24173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_2323_length_24173_cov_7.793971, whole genome shotgun sequence.
ACCESSION   DAOSLP010000013 DAOSLP010000000
VERSION     DAOSLP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 24173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24173
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_24173_cov_7.793971"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..986)
                     /locus_tag="PK460_02140"
                     /pseudo
     CDS             complement(1..986)
                     /locus_tag="PK460_02140"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
     gene            complement(1254..2231)
                     /locus_tag="PK460_02145"
     CDS             complement(1254..2231)
                     /locus_tag="PK460_02145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE53931.1"
                     /translation="MDWVEKLKHKYNVDVLYDVFLRENDSLFFLIEESHDICDKPLIK
                     LFGTCEGVGLPYCVDTIDELLSHIDDKSQICDIRGANEYRDELHREINSDQTKFVLYL
                     PLDFKGTKRYLQLEIIKMFKRNISIVMLNQIDIKKVNVETLFFELYKDSLTNLFNFST
                     LLMHIDKTEDSHYFGFLDLDYFKVVNDTYGHKVGDEILAKVGWKLIEIADETVIMYRK
                     SGDEFIFMTLGLDLNETKKLVKRIQKAIRSVELPDIKIDCSIGVAEYRHNNGFYDAED
                     AVNIADVAMYVSKSTGRGRVTYFNEEQARKVMASGPLEKTLELLESRAR"
     gene            complement(2668..2880)
                     /locus_tag="PK460_02150"
     CDS             complement(2668..2880)
                     /locus_tag="PK460_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53932.1"
                     /translation="MVDTNEIRRIFNELERQAKTLLNAANSFENALRDVDNSVDTRNF
                     LEEYKTFYGILDLKTSGLQLVRWKGV"
     gene            complement(3074..5437)
                     /locus_tag="PK460_02155"
     CDS             complement(3074..5437)
                     /locus_tag="PK460_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE53933.1"
                     /translation="MIYTLKNYQRDAVDELKGYIELGFRSKNRKEVVFKAPTGSGKTF
                     MAASLFEELAGEHSDINFCILWACPGKGELHKQSHDAVKTYLGGNPVCSLLEEDFFGS
                     RKFIKNKEIVFINWEKLIQKDKGTGAWANNLMKDQEGMNFIDVIDQTKKNGTKVVLVV
                     DESHIGSSAKARIQEFINTIIIPSILLEMSATPLNDHIDVEVNPEDVIKEGMIKEDVI
                     VNEGIKKEDKSLEEKDSELLILEKGFAKREEIVKKYAELNINVNPLVLIQIPNVDEGE
                     AKKLVIKDFLREKGITEANGKLKLWCDNHDPFDKKEIRKNNDITEYLIFKTAVATGWD
                     CPRAHILVKFREGKSETFETQTIGRILRTAEAKSYDDPLLDNAYIFTNIWTFETKQDS
                     YNPNKIKTEWTKMREGYTQLRVWAQTKLISFYRSRQEDYNSADSRFGEYLFKSFMKRM
                     GLTEEDRNNVLFNDPNRLEDAGLKLETNTGDVILEETNLGMENIAEERTITGNAVNVQ
                     MSENDVQAQYNAIIRDNLNGLAYVRSKSPINTAIMDVLSTFIKGFPRAEKVKRYMQIV
                     VNNRELFAEVISEATAEFRQMLSVNAGKKGVRYDFKIEESRAYSSETHTTLLGIKSLY
                     NPLFVLKNASGQVNYLEKNFLQYLDTQNVVEWYWENGSELMRINFGISYNNGMNTFQP
                     DFIIKFKDGSVGIFDTKPNDETNLADTTVKNEALRDYLYDINQNRGYDPKVVGGIVCQ
                     SGTQFYLFEGRDYHDYNSNHDGWVNFSEFLRKIEDAYDIEKALKKLK"
     gene            complement(5440..7134)
                     /locus_tag="PK460_02160"
     CDS             complement(5440..7134)
                     /locus_tag="PK460_02160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE53934.1"
                     /translation="MADFDIEQLLKQIKELESELKASKKYGLVWDKENTKEEVVLLCE
                     KYIPTLSPIKSLNIKKGGLSHCLIEGDNFHSLTCLNYVLQDSFDLIYIDPPYNTGSED
                     FLYNDNYINSDDTFLHSKWLNFMQKRLTLARNLLKQDGVIFISIDDNEQANLKILCDQ
                     VFGPQNFVNCVVVKMSEMSGVKMSHVEKKLPKLKEYCLVYSKNRFYYSMNPIKNNRLD
                     NLDDFAKYAKYYSKIIVDKSKPVEEWEILPLDKYLKRIGKSNLTEREILQFKLDNAER
                     MIYRTNNKSFEKIQCDKPVQKIISATGIEYIWWEGKQMLFLSDYTTTFEGDLWTDIST
                     INLNKEGGVDYQNGKKPLKLLSKIISTVKNKDALCLDFFAGSGSFGEAVLMANAEDGG
                     KRRFILCQSAEENDIAKTITYPRLKTVITGIKKDGSKYSDGLSGSLYYYKTDFIKNEA
                     NTEQAKYNLVEKVDALLCLAENIFDEVERNDYSSHFKSGDKRLFIYNDYYNAQKFEEF
                     KSRVLASQGEKIVYVYSSDNNVDNSLIEGMDVVLKPIPSKIYEIYKEIVEDIKRDE"
     gene            7263..7544
                     /locus_tag="PK460_02165"
     CDS             7263..7544
                     /locus_tag="PK460_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE53935.1"
                     /translation="MRLRELRNKSGLTQNEIANKLGVSGQTILNWENGIYEPKINQLI
                     ELADLFDVTVDYLIERKTSYKSVDAICKELERIPKENVINFIKEELQKL"
     gene            complement(7539..7757)
                     /locus_tag="PK460_02170"
     CDS             complement(7539..7757)
                     /locus_tag="PK460_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53936.1"
                     /translation="MEGINHEYVTRVHNYIVAMVYFEGLLDKGEITIEEYIEIERRIA
                     FKYKLEESIFRMEKRACDYGSTIEKIEL"
     gene            complement(7975..8051)
                     /locus_tag="PK460_02175"
     tRNA            complement(7975..8051)
                     /locus_tag="PK460_02175"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8015..8017),aa:Glu,seq:ctc)
     gene            complement(8143..8219)
                     /locus_tag="PK460_02180"
     tRNA            complement(8143..8219)
                     /locus_tag="PK460_02180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8183..8185),aa:Glu,seq:ttc)
     gene            complement(8223..8297)
                     /locus_tag="PK460_02185"
     tRNA            complement(8223..8297)
                     /locus_tag="PK460_02185"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8263..8265),aa:Asn,seq:gtt)
     gene            complement(8364..8588)
                     /locus_tag="PK460_02190"
     CDS             complement(8364..8588)
                     /locus_tag="PK460_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOE53937.1"
                     /translation="MTNLERIEQIFTELLKVEKIEPEMELKALGLDSLDLVEVMMRLE
                     EEFGIEFSNDEMLGFSTVADVVAEIERKTK"
     gene            complement(8690..8827)
                     /locus_tag="PK460_02195"
     CDS             complement(8690..8827)
                     /locus_tag="PK460_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53938.1"
                     /translation="MSELKGQILGLLLVVVIFATIAAAFKASVNNQLSEIASHESALV
                     I"
     gene            complement(8844..9182)
                     /locus_tag="PK460_02200"
     CDS             complement(8844..9182)
                     /locus_tag="PK460_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53939.1"
                     /translation="MSNIMSLVYSFILIFPTILLGVDFFIIGKIIVELETRATIVSYI
                     ISEEGGIRPSLVNELAEEGITITCEGECSYISVGETIKYQLIKTYTPIIIKNEDINIY
                     VTRTTIVGYL"
     gene            complement(9179..9784)
                     /locus_tag="PK460_02205"
     CDS             complement(9179..9784)
                     /locus_tag="PK460_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53940.1"
                     /translation="MLVKWLERSAGLLLIIVGAILYFWKKNLTLALIFAGVGFTIVVI
                     TFIPQKQKSNITLADFINVFTHFRVALDNNLNVYRALNITLTTTSGAMSALLESLINA
                     LENDHSVTPFINFSLPFKHPLVSQIMINVYMLVNHGVDPKRLWQFNYLFETLVKEHND
                     EEIALHESSYERFNISLFIGTGIMIMTLMISIMGMIGEYIV"
     gene            complement(9774..10463)
                     /locus_tag="PK460_02210"
     CDS             complement(9774..10463)
                     /locus_tag="PK460_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53941.1"
                     /translation="MIIIYGGAFFLVIVIAIFSALVLGGIKITIINALIALVVAAFLT
                     YRVTNYRKDIEKRRFMFNFMEYFILNFDIQKTVEATLTTIYPLLDVKKSRVYLTMNED
                     GMLLLEKLRLTFAHQYYESFLEMVKLINEHGGEMLKVAEVLLFSISNSETQLIKLTRI
                     DNAYLIKFIFNWFFIMLVAIVFRLALDGFLKFETLPLIYVAGMEVFIAIFLTSIVLVF
                     ENRIRRTRRVS"
     gene            complement(10460..11386)
                     /locus_tag="PK460_02215"
     CDS             complement(10460..11386)
                     /locus_tag="PK460_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE53942.1"
                     /translation="MEKVIKVIEESFLAPLLSDKNITDISYNGHKLFYFSNESGRKLY
                     GEINHKTVASFLRNVANFANERFSYADTFLDISIGKYRLSAMHYACSRFEFEESLSFA
                     LRINHNFLASNPHYLNNDVVDVLKYVMKENRSIIISGTTGVGKTTLQKYLLMLLPANT
                     RVLVIDNVLELSEVTTLNKDLDLTLWQSLTNKEEEIKSFIQKALRFHPDYLIIAESRG
                     GEMKEILQGAISGHPNIVTLHADSTALAYERVIYLAKHDDRHSLLQAFPFVIHLAKKY
                     KADGSIYRYVEKITEYNVDTDEIEILYHEVIP"
     gene            complement(11389..11754)
                     /locus_tag="PK460_02220"
     CDS             complement(11389..11754)
                     /locus_tag="PK460_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53943.1"
                     /translation="MAWSNFAFLFLFTYSTLLAINIRYHHVNEVLSSVTHDHIYHVTT
                     MDLVTYELYFEKPLVRVVVANHFNLNMKRENYEYTITFYTNGVVSEESERSRYFTINL
                     VCPLGFGVTFDKTFAYYVS"
     gene            complement(11756..12700)
                     /locus_tag="PK460_02225"
     CDS             complement(11756..12700)
                     /locus_tag="PK460_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53944.1"
                     /translation="MSVFIPLVPESNFQTSALGSYRNNPGDITQSYEVAIPFIASYTT
                     TLTYRYTVYNSANEIVYTQPSTSTRALRNVRVLITYRATAGKFTVGQNRIILYYKTSS
                     EAERRHYSYFYGYQKGVNVNLNNGSAARQLLEAEVCYLYDSTNRSDIFRKMSFSSANL
                     TPIKHFNHDLYFDFSGLFLSIGVLYDNENFYSSAALYCSNPALFPYLANVGGRAMQYL
                     NLTRAYNRLVTSVKTPLFVRADTLQIARSKYSAEYLNTDTYFFPKYHFDELQNTKFTL
                     EIKDFSYSKFNLKYEFTIQIDHPYFGTSGEHEVIINRY"
     gene            12825..13367
                     /locus_tag="PK460_02230"
     CDS             12825..13367
                     /locus_tag="PK460_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOE53945.1"
                     /translation="MIVLMGASASGKTEVAKMLGKLFSIKKVVTHTTREMRVNEVNGR
                     DYHFVTKEEFLRLKAEDYFVETVEYNDNFYGTSKAEIAMNKVLIIEPIGLKSIRALNS
                     EHIVVFFMQASRAIRRKRMINRGDNPKIATERILIDDAKFNPKNIEGIDYTIDSENQS
                     IKEIAIEVYEKYALKLVNFK"
     gene            complement(13360..14265)
                     /locus_tag="PK460_02235"
     CDS             complement(13360..14265)
                     /locus_tag="PK460_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE53946.1"
                     /translation="MYVEKFLERIPSLLNKTYVVTGANSGLGFALSTLLVSKGARVIM
                     ANRNPKRSEDAINKIRELYPAASVMVLPFDQSKKDSIEAFVNSLISHNIKPEGFVLNA
                     GIYSPAKDAKTEDGVALTFGINYLGNFIFTQTLANHGLISAETRLIYISSPAYSRKFS
                     SQILARAISGQKITRPKQYAVAKTAQKIFVLGLMTKSPLIPFENKGKIYLYHPGIAVS
                     NIARFKWKFFNKIVHRFMSVFFHPPEKAVLGALVALTTDKDLNGKILVPRGFLEINGI
                     PRIKKIDEKLLSHLPYLITETLKLI"
     gene            complement(14265..15536)
                     /locus_tag="PK460_02240"
     CDS             complement(14265..15536)
                     /locus_tag="PK460_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE53947.1"
                     /translation="MGNVIRFTIVILLFALSFFFSSSESAFSTINRLRVERDVEDGKK
                     RSKTLLRIITNYENTVQTILLGNNFVNIAVTALVTGMALAYDNAHGATGQYVAIFSVV
                     LFIVLLTIGEIVPKTLAVKLNYQMSYAYANIFMFFYYLFTPLLFPFRLISARRKKKNG
                     NEEVNGDEIYTEDELQIIVDEIEEQGLIDEETSDLVRSALEFTETEAYEIMTPRVDVF
                     SYDIEDDIKELMKEEDIFKYSRIPVYEDTIDNIIGILRTKDLLKLSLSDKKINVKSLI
                     KPPLFVHHTKNVSQILTEFKQTKNHIAIVIDEYGGTEGIITMEDILEEIIGEIWDEAD
                     EVDEPIVKLEDNHYIIDGGLNIEDFFELFELSEDEDNDYDTVAGWCIEMLDRFAKAGD
                     EFTYEKITVKIIEVEGFVVEKVEVIVESEED"
     gene            complement(15529..16545)
                     /locus_tag="PK460_02245"
     CDS             complement(15529..16545)
                     /locus_tag="PK460_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOE53948.1"
                     /translation="MIDDPQTWVLLILYFVAGMYFSSSETAFMTANRFKIRAKAEEGS
                     VSAKLAAWVINRLENSIIAVVVFNNLAHLLFTALLTVVLVNQYGNDTGELLATLISVP
                     LMFLFAETIPKVLGRRYSEQYALISAYLLVPFIIISYPVALFFRGIVWVVKKLFKATE
                     DNIFTADDFEQVIETIEEHGIINEDASDIIISSLEFSETVVKDVITPKNAIVAYDISK
                     FNKDSFKDFVLSTHFSRIPLYDGSLDKIVGVILVREYIKHYQKKPNINPRTIMARPYY
                     VNYKITLTKMVEGFKKNNTHIAFVVDDKKKLLGMVTMEDVLEELVGQIAETSGTVKGA
                     SVNG"
     gene            complement(16636..18939)
                     /locus_tag="PK460_02250"
     CDS             complement(16636..18939)
                     /locus_tag="PK460_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOE53949.1"
                     /translation="MKKIFNFIVRFKVIFFGFWIALTIASIFMIPHVKINYDTTKYLP
                     EDTRVKESLRVMEEEFGITGQASVMIEKVETLQEVFVYKDEIISIPTVMEVVWIDSFV
                     ERDTINDIQRIAEENGMSGVFDFANIPGLNAFYKDKSALLQIVFTEEDHSRAVGKAIE
                     QIRSYLEGIKRPYAMRGTAVNAYYTQKLTESEVLRVTVIVVPIILFILLVFTRSWAEP
                     IIFLIAVGVAVLVNMGTNLFFPSVSFLTNSTASLLQLAISMDYSIFLLHAYQKERAEG
                     LDKKDAMVMAMRKSFVSINSSMLTTAAGFLALMFMRYTIGIDLAAVMIKGILLSIIST
                     FTFMPGLILLTDKIVEKTKHKRFFPLLGGLTKWIVKLRYVVPAILLLVIVPSYLAQTN
                     NSFVYGEAAMSVSEGTPAAIEKNRIEAKFGKENLMAILVPRDEADAYIAQEKAMIKQL
                     YREFERANQDFVVQITSLSNLSDIETYIPNYDDLDQTIKNFLDDIFADIMDNLITEEY
                     KAQFLSEHYSRIIITVNTESESPRAEIAVDIVETVVARQPFARDAHIIGVSPSVKEIR
                     NVVETDYVIVDLISIGFVFIILLVSFRSISLPFILVLVIQISIWINMAIPYLTSQPMI
                     FIGYMIVSSVQLGATIDYAILFTGFYTEGRKTMIRRDAIKYALDSGGHSILTSCLILT
                     SAGYSLKFLSSIEGVSSLGELIGRGAALSGILVLVLLPQLLYIFDKVVEKTTWKMRFY
                     KGGTKVGRVIIEDEGYSNSDQDPTLLS"
     gene            complement(18936..19502)
                     /locus_tag="PK460_02255"
     CDS             complement(18936..19502)
                     /locus_tag="PK460_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE53950.1"
                     /translation="MQLLKPELNSLIQKEALDLFYKNGYQATLMRDLAAAVGMSVGNT
                     YRYYKSKHDLFTTLVQPVHEKLVELLEATHDNPEAHFNELCLTTIMTRFTAICSKYHK
                     ESVIFLEYYLAQEDDPVIQNFRGLIYRTVKAHLPQITMPMFDLLYHFIATGTVFVLRK
                     SAPENIVSNLKLLYVFLFKDVKDRINAL"
     gene            complement(19610..20614)
                     /locus_tag="PK460_02260"
     CDS             complement(19610..20614)
                     /locus_tag="PK460_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53951.1"
                     /translation="MKLKKFMLAIGVIGLLLTGCDDPNTSSSQTSGSETSEITEAERF
                     SAAGYSPLKGWPVESIRMHYTREGIDAAWVFTNVLTPTGFGQNTDLYVRSYEDELGID
                     VFKSFEVVRVTADATTFTNYVALYDGKTDYTVIVDEEHEFGIEAFSGKVRIGVSFVEE
                     NEAVPSATYFRFTVQEKEEFPGAPKVDRSAHRSEITFSNDFKVTTRSKEIVEWAFSDL
                     YKFKVEVGESSTNVGNIPNNSGVGYLTPQLRIYKDQVLTLNMEQGTMKQVIFHGVNYT
                     EDGVDVKSIDAFPVVEGAQQFKTANAVLYHFDADNTTSLSLVVTANARLLDVTVIYFL
                     "
     gene            complement(20759..>21427)
                     /locus_tag="PK460_02265"
     CDS             complement(20759..>21427)
                     /locus_tag="PK460_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53952.1"
                     /translation="EEEEELIVELDLEDPKLEALIRKLANEEIDKRPVPEKEVVVREV
                     EKVVEVKPEPKPEPKPEPKPEPKPEPKPVVVAKPKAEAKPKIERVSFPDRMQMVEDSA
                     KANYNLLKNYLLSYGLNSRVSNVADSFRLGRVLYARLTNSGNSGLKLYLPLDLEDYKD
                     SKIPLKSAEGIKQYEDVPVFLYVRSDLSVKRAQELIDDVMLKHGIARKHDMKEVDHVK
                     ELVK"
     assembly_gap    21428..21527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21528..22141)
                     /locus_tag="PK460_02270"
     CDS             complement(<21528..22141)
                     /locus_tag="PK460_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53953.1"
                     /translation="MQKKRTSAVLTLILAILMLTFFGLAHIVSTVYLVDGAPIGVLGM
                     LVKFKEVLLSLFVPNFGDLMSLAGFVATVVVLGVALVLALVWAIKLLYYRRRGWNLLS
                     PLLFLIAIFLGVLLVGSHTDVDLAFRQPTAQVATAYFFVVGVLMVSLSLHLVVVGLKT
                     AGTKKVASLLEEDLDDEYVVIDEQVEERVVEEKEEEPVVEEKVE"
     gene            complement(22251..23918)
                     /gene="gltX"
                     /locus_tag="PK460_02275"
     CDS             complement(22251..23918)
                     /gene="gltX"
                     /locus_tag="PK460_02275"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOE53954.1"
                     /translation="MSREKLANIIFPEITTTIADLEERYPHRSLKDGAEVTRFAPSPT
                     GFLHTGSLFAALVSRTVAHRSGGVFFVRLEDTDTKREIAGAGENLLNEMKIFKVYPDE
                     GYLGDSELGDYGPYKQSERALIYKTVIKQMIKDDLAYPCFCTTDDLALLRATQEASKV
                     VSGYYGHYATCSKLSADEAIAKIEAGVPYVIRFRSKGNHENRVSFDDEVKGTIEIAEN
                     DQHIVILKSDGLPTYHFAHVCDDHFMRTTLVSRGEEWISSVPLHLEMFAAVKFNQPRY
                     AHLPTIDKLDDGNKRKLSKRRDPEAAVTYFLELGYPVDAVLTYLMSIANSNFEEWWAE
                     NREKPLSEFPFSISKMSVVGALFDIEKVNFFAREYIASLTAKEVVDEYLAYALYNKNE
                     KLVKRIKDNYDLFVQILNIEREGEKPRKDYHNYSMVYPSIKFFFSDEFAAMHEDFDIG
                     EFKESLSKDTLIGLLESFKTELTYGEGNSVWFDSLKKVAEKHGFAINNKAYRTNPELY
                     NGNVADCAEVLRLAIVLSKKSLNLYEVLSILGPKEVEKRLTAMQNKL"
     gene            24038..24111
                     /locus_tag="PK460_02280"
     tRNA            24038..24111
                     /locus_tag="PK460_02280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24070..24072,aa:Gly,seq:gcc)
BASE COUNT         7811 a         4620 c         3702 g         7940 t
ORIGIN      
        1 gatattttaa tgtctatatc tttttttgtt agattgaata ccacgaatac tccgtggtga
       61 tattccctta aataaacctt gttatttttc gaggtatagt aatcgctcac aggatataat
      121 ttgatattaa gttcaaattg actaattgtt aaagttgaat aatagctatt ttccgtaaat
      181 aaccagttag agtcacatag atatagatca gtttgtgaaa catacacttg cttatatcca
      241 taattagcac ctaaaaatcc tttagcgtta aaagtaatcg cctgcgggtc agcggctaat
      301 ttaagtccaa ttatctttgt cattccgtgc accggtgtat cttcaaaata ataaatatcg
      361 ttatatccaa tattagtttc agtgccatct aataaactta tttccggacg cggatcttct
      421 tcatcaatcg taatatattc ataaccaaca aagaataagt tattatcaat tacacggtgg
      481 tcgataataa tattgtcctt aatatttagt tcatacacta cttgatgatt cgtccgttca
      541 ataacagtaa ttgctgtcgt ggcataccca aaccatcctg gcagccaaaa ctcttcatat
      601 tctggaagta aaacatattc atacttttca ctgataacta cgacctattc cggtagcaaa
      661 tacatcccaa taccgtacga atccgcaaaa gtgagtgatt tacttaatgt aatgctttta
      721 tcatcagcaa catcaaatac ttgaagcgcg ccgcgttcac gcgctaaata ataaatttga
      781 tagccatcag ttttaataat gtcagcttca ctaacacctg ccacttgttc gtttgtacca
      841 gtgaaatcgc gacctttcgt cccagcgccc gtatcagatt ctggaacagc atcaccccca
      901 gcgattgtat cgtaatattc attcgtggac ttgttacctt ctttgactaa ttcattaaat
      961 ttttgtttcg tttttacttg ttgcattttc gttttggtaa ttaagtcgct attagtttaa
     1021 caactcgcta aacttgttaa cattcccaaa aagaatagtg atactataaa ccgtgaaagt
     1081 tttggtttaa acgatttttt catattattt tcctttcagt aattaagtgg tatacactta
     1141 actcgcgttt aaaacggatt actatctact attattttgc atcgtttttg aattttcgtc
     1201 aatattatta aaaaagacac tcgaagtgtc tttaattact agttaacaga ttattatcgc
     1261 gcgcgtgatt cgagtaattc taaagtcttt tctaaaggtc cagaggccat aacttttcgc
     1321 gcttgttctt cattaaaata tgtcacacgg ccgcgaccag tggattttga tacatacatg
     1381 gcaacatcag caatattgac agcatcttca gcgtcataaa acccgttatt atgacgatat
     1441 tccgcaacac caattgagca atctatttta atgtccggta attcaactga gcgaatcgct
     1501 ttttgaatac gttttacaag tttttttgtt tcatttaaat caagccctag tgtcataaaa
     1561 ataaattcat caccactttt gcgatacatg ataactgttt catccgcgat ttcaattaat
     1621 ttccatccta ctttagcgag aatctcatcg ccaactttat gaccatatgt atcatttaca
     1681 actttaaagt aatctaaatc taagaatccg aagtaatgac tatcttccgt cttatcaata
     1741 tgcattagta atgtgctgaa attaaataaa ttcgttaaac tatctttata caactcaaaa
     1801 aagagtgttt caacattaac ttttttaata tcgatttgat taagcatcac aattgatata
     1861 ttacgtttaa acatcttaat aatttcaagt tgtaaatatc ttttagtgcc tttaaaatca
     1921 agtggaagat agagcacaaa tttagtttga tcgctattaa tttcacggtg taattcatca
     1981 cgatactcat tagcgccgcg aatatcacat atttgacttt tatcatcaat atgacttaat
     2041 aattcatcaa tcgtatcaac gcaataagga agaccgacac cttcacatgt accaaacagt
     2101 ttaattaaag gtttatcaca aatgtcatgg ctttcttcaa taaggaaaaa aagtgaatca
     2161 ttttccctta aaaacacatc atataaaaca tctacattat atttatgttt tagtttttca
     2221 acccaatcca ctatgaaatc acctcttatt aataaattag taatttatta ttgcacgatt
     2281 ttatgcaaaa ttccataagt atatatgaat aattgaacat tttttacatt tgttcaagtt
     2341 gatgtgtcaa catagacatt ttggtatggg gccggagtca tattttttaa tgatgaatgg
     2401 tatcatttat tattgtgcca atatacataa ctaggtaaaa ttgtataagc actatcgatt
     2461 gcttttatat taatatttgc ataaccgtta taaattttta ttttaactaa aattatctat
     2521 aagtatcatt tccaccataa atagatattc catcaaatgc atctataaaa tcctcacagt
     2581 ttttaacaaa agttcggcag ttatcaacat atctataaaa actataatta aaatcaggac
     2641 caaaacgccc cgaataatag tggttatcta aactcctttc caacgaacga gctgtaaacc
     2701 tgatgttttt aaatccaaaa ttccataaaa tgtcttgtat tcctctaaaa aatttcttgt
     2761 gtcaactgag ttatcaacat cccttagagc attttcaaaa gaattcgcag catttaataa
     2821 agtctttgct tgtctttcta gctcgttaaa aattcttcta atctcatttg tatctaccat
     2881 aacttttacc tcttttcatt aatctttaaa ctaattgtaa gccaaataaa ctttaaaacc
     2941 caaatttaat ataaaaaaac ggcacgcaaa ctgtgccgtt acacgaaagt ttggcctaaa
     3001 tggacagttt tgatacagtg gaacaaaacc gaacgctttg gaacactgac cgaacactat
     3061 tatcatagag gcattatttc agctttttaa gagctttttc tatatcatac gcatcttcaa
     3121 tcttacggag gaattcactg aagttcaccc atccatcatg atttgagtta tagtcatgat
     3181 agtctcttcc ttcaaagaga tagaattggg ttcctgattg acaaacaata ccaccaacta
     3241 cttttggatc ataaccacga ttttggttta tatcgtataa ataatctctt aatgcttcgt
     3301 tcttaaccgt ggtatcagct aaattggttt cgtcgtttgg ttttgtatca aagataccga
     3361 cactaccatc tttaaactta atgataaagt ctggttggaa agtattcatt ccattgttat
     3421 aggaaatacc aaagttgatt ctcattaatt cacttccgtt ttcccaatac cactcaacga
     3481 cgttttgagt atctaaatat tggaggaaat tcttttcgag atagttaact tgtccgctag
     3541 cattcttcaa aacgaacaat gggttatata aagatttgat tcctaaaaga gttgtgtggg
     3601 tttcagacga ataagccctg ctctcctcaa tcttaaagtc atatctaaca ccttttttac
     3661 cagcgtttac agaaagcatt tgtctaaatt ctgcagttgc ttcagatata acttcagcga
     3721 ataattctct gttattaaca acgatttgca tgtatctttt gactttttct gctctcggga
     3781 atcctttgat gaatgtgctt aaaacatcca taatcgctgt atttattggt gacttagatc
     3841 taacataggc caagccattt agattgtctc taataattgc attatactga gcttgaacat
     3901 cattttcaga catttgaaca ttaacagcgt tgccggtgat ggttctttct tccgcgatgt
     3961 tttccatacc taaattagtt tcttcaagga taacatcacc agtatttgtt tctaatttga
     4021 gaccagcatc ttctaatcta tttggatcgt tgaataatac gttgtttcta tcttcttcag
     4081 tcaaacccat tctcttcata aaagatttga ataaatattc accaaatcta gagtcggcag
     4141 agttatagtc ctcttgtctg gatctataga atgaaatcaa tttagtttga gcccatactc
     4201 ttagctgagt gtaaccttct ctcattttgg tccactccgt tttaattttg tttgggttat
     4261 atgaatcttg cttggtttcg aatgtccaaa tatttgtgaa aatatatgca ttatcaagca
     4321 atggatcatc ataagatttg gcttctgcag ttcttaaaat acgaccaatt gtttgagttt
     4381 caaatgtttc agatttgcct tcacggaact taactaaaat atgtgctctt ggacaatccc
     4441 atcctgttgc taccgcagtt ttgaatatta aatattcagt gatgtcgttg ttctttctaa
     4501 tctctttctt atcaaaagga tcatgattgt cgcaccatag ctttaatttg ccgtttgctt
     4561 ctgtgattcc tttttctctt aagaaatctt tgataacgag tttctttgct tcgccctcat
     4621 ctacattagg gatttggata aggactagcg gattaacatt tatatttaac tcagcatatt
     4681 tttttactat ttcttctctt ttagcaaagc ctttttcgag aataagaagt tcactatctt
     4741 tctcttctaa agatttatct tcctttttaa tgccctcgtt aacgatgaca tcttctttaa
     4801 tcatcccttc cttaataaca tcttcaggat taacttctac atcaatgtga tcgttaagtg
     4861 gggtcgcaga catttctagt aaaatgcttg gaataatgat tgtattgatg aactcttgaa
     4921 ttctggcttt cgcagaagaa ccaatatgag attcatcaac gaccaaaaca accttagtac
     4981 cattcttctt ggtttgatca ataacatcaa tgaagttcat accttcttgg tctttcatta
     5041 agttattggc ccatgcacca gttcccttat ccttttggat aagtttctcc cagttgatga
     5101 acacaatttc tttattttta atgaattttc tggagccaaa gaagtcttct tcaagcaaag
     5161 agcaaacagg attaccacct aagtatgttt taactgcgtc atgagattgc ttgtgaagtt
     5221 caccctttcc aggacaagcc caaagaatgc agaaattaat gtcgctgtgt tcgccagcta
     5281 attcctcgaa caatgatgca gccataaagg ttttaccact tcctgttggt gctttaaaca
     5341 ctacttcttt tctatttttt gatcgaaagc ctaattcgat gtagcccttt aattcatcta
     5401 ctgcatctct ttgataattc ttaagtgtat aaatcataac tattcatccc tttttatatc
     5461 ttcaacaatc tctttgtata tttcatatat ctttgatgga attggtttta gaacaacgtc
     5521 cattccctca attagagagt tatcaacatt gttatcgctt gagtaaacat aaacgatttt
     5581 ttctccttga gatgccaaaa ccctgctctt aaactcttca aatttttgag cattgtaata
     5641 atcgttataa atgaataagc gcttgtcacc actcttaaaa tgagatgaat aatcatttct
     5701 ctcaacttcg tcaaaaatgt tttcagccaa acataataag gcgtcaactt tttctacaag
     5761 attatatttt gcttgttcgg tattggcctc gtttttaata aaatcagtct tatagtaata
     5821 aagacttcca gacaaaccat cgctgtattt tgaaccgtct tttttaatac ccgtaataac
     5881 ggttttaagt cttggataag ttatagtttt tgcaatatcg ttttcttcag ctgattgaca
     5941 aagaataaag cgtctctttc cgccatcttc tgcatttgcc attaaaacag cttctccgaa
     6001 tgaaccagaa ccagcgaaaa agtccaaaca taaagcatct ttgtttttga ctgtagatat
     6061 tatcttagat aataatttaa gtggcttttt accattttgg taatccacac caccctcttt
     6121 atttaagttg atggtgctta tgtcggtcca tagatctcct tcgaaagttg ttgtgtagtc
     6181 tgacaggaaa agcatttgtt taccttccca ccaaatgtat tcaattcctg tagcactaat
     6241 gattttttga accggcttat cacattggat tttttcaaag ctcttattgt ttgttctata
     6301 aatcatcctt tcggcattat ctaatttaaa ttggagaatt tctctttcgg ttaaatttga
     6361 tttaccaatt ctcttaagat acttgtcaag aggaagaatc tcccattctt cgacaggttt
     6421 gcttttatcg acaataattt tactgtagta cttcgcatat ttagcgaaat cgtctaaatt
     6481 atctagtctg ttatttttta taggattcat tgaataatag aacctatttt ttgaataaac
     6541 caaacaatat tcttttagct taggaagttt cttttcaacg tgtgacattt tcactccaga
     6601 catttctgac attttcacaa cgacacaatt aacaaaattt tgtggtccaa agacttggtc
     6661 acacagaatc tttaagttag cttgttcgtt atcatcaata gaaataaaaa tgacgccatc
     6721 ttgtttcaaa agattcctag caagagtcaa tcgtttttgc atgaaattta gccatttgct
     6781 atgcaaaaaa gtatcgtcgc tatttatgta attatcgtta tataaaaagt cttcactacc
     6841 ggtgttatat ggcggatcaa tgtaaatcaa atcaaacgaa tcttgaagaa cataatttaa
     6901 gcaagtgaga gaatgaaagt tgtcaccctc aatcaaacag tgagataggc cgcctttttt
     6961 aatgttcagg cttttgattg gcgagagtgt tggaatgtat ttttcacaga gcaacacaac
     7021 ttcttctttt gtgttttctt tatcccaaac aagaccgtat tttttagacg cctttaattc
     7081 ggattctaac tctttaattt gctttaataa ttgctcaata tcaaaatcag ccatagttca
     7141 acctcataga ttagtaaaat caaggtttcc ttgaattcac actatattct acaaagttta
     7201 acttgatttg tctatattca aatcaaggtt tctttgatta tttgtctaag aggaaaagat
     7261 aaatgagatt gagagaacta agaaataaga gcggtctaac gcaaaacgaa atagctaata
     7321 agcttggtgt ttctgggcaa actatactta attgggaaaa tggtatttat gaaccaaaga
     7381 ttaaccaatt aatagaatta gctgatttat ttgatgtaac tgtcgattac cttatcgaaa
     7441 ggaaaacaag ctataaaagc gttgatgcta tttgtaaaga attagaacga attcccaaag
     7501 aaaacgtcat taactttatt aaagaagagc ttcaaaaatt atagttcaat cttctcaatt
     7561 gttgagccat aatcacaggc tcttttttcc attctaaaaa tgctttcttc aagcttgtat
     7621 ttaaaggcaa ttcttctttc aatctctatg tactcttcga ttgtgatctc acccttatct
     7681 aacaagccct caaaataaac catagctaca atgtaattat gtactctagt tacatattca
     7741 tgatttatgc cttccatctt agtggacctc ctattatttc cactaatcgc tctaaatcta
     7801 aaaaatgtga agaaaaacat tcctaatggt gaatagttgt atttttttgt atcaaaatac
     7861 tgtgaataac taccctacag gcgaaatata ttgcggaaaa atatctcaaa aaagtgcgat
     7921 gtaatcaaag aaaaaaggct aataccgata tatattaacc ttcaattttt aagttggtga
     7981 cccgtacggg attcgaaccc atgaatgcat gcgtgagagg catgagagtt aaaccgcttc
     8041 tccaacgggc cataatcgcg attattattt tagcacttac aaaataagtg tgtgcaatga
     8101 aaacaaaaaa acggcacttt atagtaccgt ttgatttacc tatggcgacc cgtacgggat
     8161 tcgaacccat gaatgcatgc gtgaaaggca tgtgagttaa accgcttctc caacgggcca
     8221 gctggcgcct agtgtaggat ttgaacctac gaccgatcgg ttaacagccg attgctctgc
     8281 cgctgagcta actaggcatg ttagacctag gcagtcataa gaatatcaca cttcctaagt
     8341 cttggcaata ataattatta ttattattta gttttacgct caatttcagc gacaacatca
     8401 gcgactgtgg aaaatccaag catttcgtcg ttactaaatt caataccaaa ctcttcttcg
     8461 agtctcatca tgacttccac taaatcaagg ctgtcaagtc ctaatgcttt taattccatt
     8521 tcaggttcaa ttttttcaac cttgagcaat tcggtgaata tttgttcaat acgttctaaa
     8581 tttgtcatag tattctcctt agaattcaaa ttttaacaaa tagcacgcat tatttcaaat
     8641 aaaaacgatt ccaaggtttc ttcactgcta agcgacgaat aaaagttaat taaattacta
     8701 acgcgctttc gtgactagca atttctgaaa gttgattatt aactgatgct ttaaaggccg
     8761 cagcaattgt ggcaaaaata acaacaacaa gtaataaccc taaaatttgt ccttttagtt
     8821 ctgacataaa attacctcct taattataaa taaccaacaa ttgttgttct tgtcacataa
     8881 atgttaatgt cttcattttt aataataatc ggagtgtatg ttttaattaa ttgatattta
     8941 attgtttccc ctacactgat gtaagagcat tcgccttcac aagtaatcgt tatgccttct
     9001 tccgctagtt cgttaactag acttggccta atgccccctt cttcactaat aatatagcta
     9061 acaattgttg cccttgtttc tagttcaaca atgattttcc caatgatgaa aaagtcaaca
     9121 ccaagcaaaa ttgtcgggaa aattaggatg aatgaataaa caaggctcat aatattactc
     9181 atactatata ttctccaatc atccccataa tacttatcat cagtgtcata atcataattc
     9241 ctgtaccaat aaataaacta atgttaaagc gctcataact actttcatgt agcgctattt
     9301 cttcatcatt atgttctttc actagtgttt caaataaata attaaattgc cacaaccttt
     9361 ttggatcaac accgtgatta acaagcatat aaacattaat cataatttgc gaaacaagcg
     9421 gatgcttaaa gggtaaggaa aaattaataa aaggggtaac actatgatca ttttctaaag
     9481 cattaatcaa tgattctaaa agagcggaca tcgcaccact tgtcgtcgtt aatgtaatat
     9541 ttaaagcgcg atatacattt aagttattat caagcgctac acgaaaatgg gtgaatacat
     9601 taataaaatc cgctaaagtg atattactct tttgcttctg ggggataaag gtaataacaa
     9661 caattgtaaa accaacacca gcaaatatca gtgcaagtgt taagtttttc ttccagaaat
     9721 ataaaatagc gccaacgatg attaataata acccggcact acgttctaac catttaacta
     9781 acacggcgtg ttctcctgat tctattttca aatactaaaa caatgcttgt taaaaatata
     9841 gcgataaaga cttccatacc cgcaacataa attagtggta atgtttcaaa ttttaaaaag
     9901 ccatcaagcg ctaggcggaa gacgattgct actaacataa taaaaaacca attaaaaata
     9961 aatttaataa gataagcatt atcaattcta gttaatttaa taagttgcgt ttcactatta
    10021 gaaatactaa aaagcagtac ttccgctact ttaagcattt caccaccgtg ttcattaata
    10081 agtttcacca tctcaagaaa tgattcataa tattgatgag caaaagtaag tcgcagtttc
    10141 tcaagtaaaa gcatcccgtc ttcattcatt gttaaatata ctcttgattt ttttacgtca
    10201 agcagtggat atattgtagt tagtgttgct tctactgttt tttgaatgtc aaagttaaga
    10261 ataaaatatt ccataaaatt aaacataaag cgtctttttt caatgtcttt gcgataatta
    10321 gtaacgcgat atgtaagaaa agcagcgaca actagtgcaa ttagtgcatt gataatcgtt
    10381 atttttatac cgcctagcac tagggcacta aatattgcta ttactataac caaaaagaaa
    10441 gcaccaccat aaataataat cacggaatca cctcgtgata aagtatttca atttcatcag
    10501 tgtcaacatt gtattcagtg attttttcga cataacgata aatcgagcca tctgccttat
    10561 atttttttgc taaatgaata acaaacggaa aagcttgtaa taaactatga cgatcatcat
    10621 gctttgctaa atagatgacg cgttcgtatg ctaaagctgt actatcagcg tggagtgtta
    10681 caatatttgg atgaccgctt attgctcctt gcagtatttc tttcatctca ccaccccgac
    10741 tctcggcaat aattaaataa tcgggatgaa accgtaaagc cttttggata aaacttttaa
    10801 tttcttcttc tttatttgtt aatgattgcc aaagtgttaa atctaaatct ttatttaatg
    10861 ttgtgacttc acttaattca aggacattat caatcactaa cacccgcgta ttagcgggta
    10921 aaagcattaa taaatacttt tgtaaagtcg ttttaccgac tccagttgtg ccgctaatga
    10981 tgatggagcg attttctttc atcacatact ttagaacatc aacaacatca ttatttaaat
    11041 aatgcggatt acttgcgaga aaattatgat taatgcgcaa ggcaaaactt aaactttctt
    11101 caaattcaaa tctactacat gcataatgca tcgcactaag gcgatacttt ccaattgata
    11161 tatcaagaaa tgtatcagcg taagaaaatc tctcattagc aaaattagcg acattacgta
    11221 aaaagcttgc gacagtctta tgattaattt cgccatataa cttgcgacca ctctcatttg
    11281 aaaagtaaaa taatttgtga ccgttataac taatatctgt tatgttttta tcactaagga
    11341 gcggggctaa aaaactctct tcaattactt taattacttt ttccattgtt aactcacata
    11401 ataggcaaat gttttatcaa aagtcacacc aaagcctagt ggacacacga ggttaattgt
    11461 aaaatatcga cttctttcac tttcttcact aacgactcca tttgtgtaaa atgtaatcgt
    11521 atattcgtaa ttttctcttt tcatattaag gttaaaatga ttagcgacta caactcttac
    11581 aagcggtttt tcaaagtaga gttcataagt aactaaatcc attgttgtca catgataaat
    11641 atgatcatga gttacagaac taagtacttc attaacatgg tgatagcgaa tatttatcgc
    11701 tagcagtgtg ctataagtaa ataaaaataa aaaagcaaaa ttactccatg ccatactagt
    11761 acctattaat tattacttca tgttcaccgc ttgtgccaaa gtaagggtga tctatttgaa
    11821 tggtaaattc gtattttaga ttaaatttac tataactaaa atctttgatc tctaatgtaa
    11881 acttcgtgtt ttgtaactca tcaaaatgat acttaggaaa gaaatatgta tcggtattta
    11941 gatattcggc actatattta ctacgcgcaa tttgtagtgt atctgctctc acaaacaatg
    12001 gtgttttcac actcgttact aaacgattat aagcacgtgt taagtttaaa tactgcattg
    12061 ctcttccgcc aacattagca agataaggaa agagagctgg gttgctacaa tataaagccg
    12121 cactgctata aaaattttcg ttatcataaa gcaccccaat tgataaaaaa agcccactaa
    12181 aatcaaaata taagtcatga ttaaaatgtt taataggcgt taaattagcg gagctaaaac
    12241 tcatcttccg aaatatatca ctgcgattag tactatcata taaataacat acttcagctt
    12301 ctaataattg ccgtgccgca ctaccattat ttaaattaac attgacacct ttttgatagc
    12361 cataaaaata tgaataatga cgacgctctg cttcacttga tgttttgtaa tataaaataa
    12421 tacgattttg accaaccgta aactttcctg ctgtagcacg atatgtgatt agcacccgaa
    12481 cattacgcag tgcccttgtc gatgttgatg gttgagtata aacaatctca ttagcgctat
    12541 tgtatacggt atagcggtat gttaatgttg tcgtgtaaga agcaataaaa ggaatcgcca
    12601 cttcataact ctgtgtaata tctccaggat tattacgata actaccaagc gctgaagttt
    12661 gaaaattact ttcaggtaca agcggaataa acacgctcat aaaaatcgtt aaccacaatg
    12721 ttttaaagta tctcatggta ataaataaaa gagtattttg aactttttca catttacgac
    12781 tgattcttcg tgcaattaac aaaattttat tttattattt atgtatgatt gtactaatgg
    12841 gagcaagcgc aagcggaaag acggaagttg ccaaaatgct tggcaaactt tttagtatta
    12901 aaaaagttgt gacgcacaca actcgtgaaa tgcgtgttaa cgaagttaat ggtcgcgatt
    12961 atcactttgt cacaaaagaa gaatttttac gacttaaagc tgaagactac tttgtagaga
    13021 ctgtagagta taatgataac ttctacggaa cttcaaaagc tgaaatagcc atgaataaag
    13081 tattaattat tgaacctatt ggtttaaaat caataagagc cttaaatagc gaacatatcg
    13141 tcgtcttttt catgcaagct agtagagcta ttcgccgtaa acggatgatt aatcgcggtg
    13201 ataatccaaa aattgccact gaacgtattt taatcgatga cgctaaattt aatccaaaaa
    13261 acatcgaagg tatcgattat acgattgata gtgaaaatca aagcataaaa gaaattgcga
    13321 ttgaagtata tgaaaaatat gccttaaaac tcgttaattt taaataagct ttaatgtttc
    13381 tgttattaag taaggtagat gagataaaag tttctcgtct atttttttaa tacgcggaat
    13441 cccgttaatt tcaaggaacc cacgcggcac taaaattttc ccatttaaat ctttatctgt
    13501 cgttagcgcc acaagggcac caaggacggc tttttcggga ggatgaaaaa acacactcat
    13561 aaatctgtgg acaatcttgt taaagaattt ccatttgaag cgtgcgatat tactaacagc
    13621 tattcctggg tgataaagat aaattttacc tttattttca aacggaatta gcggagactt
    13681 agtcattaag ccaagcacaa atattttttg ggcagttttc gcaacagcat attgtttcgg
    13741 ccgcgtgatc ttttgtccgc ttattgcccg tgcaagtatt tgcgagctaa acttacgcga
    13801 atatgctgga ctagaaatat aaattaacct tgtttctgca ctaattaacc cgtgatttgc
    13861 aagtgtttgt gtaaaaataa agtttcctaa gtaattaata ccgaatgtaa gcgcaacacc
    13921 atcttcagtc ttagcatctt tagcaggcga ataaatgcca gcattaagca caaagccctc
    13981 aggcttaata ttgtgactaa ttaaactatt aacaaaggct tcaatagaat cctttttact
    14041 ttgatcaaaa gggagtacca ttacactcgc agcaggatat aattcgcgta tcttatttat
    14101 tgcgtcttca ctccgtttag gattacgatt agccataata acgcgtgccc ctttacttac
    14161 aaggagtgtg ctaagggcaa aacctaaacc actattagcg ccagtaacga cataagtttt
    14221 atttaataaa gacgggattc gttctaaaaa cttttcaacg tacattaatc ttcttcactt
    14281 tcaacgataa cttcaacttt ttcaacaaca aagccttcaa cttcaatgat tttgacagtt
    14341 attttttcat aagtgaattc gtcgcccgcc ttagcaaaac gatcgagcat ttcaatacac
    14401 caaccagcga ccgtatcata atcattatct tcatcttcgc ttaattcaaa taattcaaag
    14461 aagtcttcaa tatttaagcc accatcaata atgtaatgat tatcttcaag cttaacaatc
    14521 ggctcatcaa cttcatcagc ctcatcccaa atttcaccaa taatttcttc aaggatgtct
    14581 tccattgtaa taataccctc ggtgccacca tactcatcaa tgacaatggc aatatggttt
    14641 ttcgtttgct tgaattcggt taaaatttga ctaacatttt tcgtatgatg gacgaaaagt
    14701 ggcggtttaa ttaagctttt cacattaatt ttcttatcgg aaagtgaaag tttaagtaag
    14761 tcttttgtcc gtaaaatacc aataatatta tcgattgtat cttcgtaaac ggggatacga
    14821 ctatatttaa aaatatcttc ttctttcatt aactctttaa tgtcatcttc aatatcataa
    14881 ctaaagacat caacacgcgg cgtcataatt tcgtaagcct ctgtttccgt aaattcaagc
    14941 gcactgcgca ctaagtcact tgtctcttcg tcaattaagc cttgctcttc aatttcatcg
    15001 acaataattt gtaattcgtc ttccgtataa atttcatcgc catttacttc ttcattacca
    15061 ttctttttct tccggcgtgc actaattagt ctaaacggaa ataaaagcgg cgtaaataaa
    15121 taataaaaga acataaagat attcgcgtaa gcatacgaca tttgataatt taatttaaca
    15181 gcgagtgttt ttggcacaat ttcaccaatc gttaaaagga caataaataa aacaacagag
    15241 aaaatagcta catattgtcc agtagcgcca tgcgcattat cataggctag cgccatccct
    15301 gtcacaaggg cggtaactgc aatatttaca aaattgttac caagtaatat tgtttggaca
    15361 gtattctcat aattcgtaat tattctcagc agtgttttgc tacgcttttt accgtcttcc
    15421 acatcacgtt ccacacgaag ccgattaatc gtggaaaaag cactctctga actcgagaaa
    15481 aagaaactaa gcgcaaataa aagaataaca attgtaaatc taattacatt acccattaac
    15541 gcttgctcct ttaacagtac cacttgtttc cgcaatttgg ccgactaatt cttcaaggac
    15601 gtcttccatt gtaaccatac caagtagttt ctttttatca tcgacgacaa aagcaatatg
    15661 cgtgttgttc ttcttaaagc cttcaaccat tttagtaagc gtgatcttat aattaacata
    15721 atatgggcgt gccataattg tgcgtgggtt aatatttggt ttcttttgat aatgtttaat
    15781 atattcccgc actaaaataa cgccaacaat tttatcaagt gaaccgtcat acagcggaat
    15841 tcttgaaaaa tgtgttgata gcacgaaatc tttaaatgaa tctttattaa atttactaat
    15901 gtcataggca acaatcgcgt ttttcggtgt gataacatct tttaccactg tttcactaaa
    15961 ttcaagggag gagataataa tatcactagc gtcttcatta ataatcccgt gctcttcaat
    16021 tgtttcaatg acttgctcaa aatcatcagc ggtgaaaata ttatcttctg tggctttgaa
    16081 aagtttttta acaacccaga caataccacg gaaaaaaagc gcgacaggat agctaataat
    16141 aataaatggc actaaaagat aagcgctaat taatgcgtat tgttcactat agcgacgtcc
    16201 taatactttt ggaatcgttt ccgcaaataa aaacatcagc ggcacactaa ttaaagttgc
    16261 aagtaattca ccagtgtcat tgccgtactg attaacaagg acaacagtta aaagcgcagt
    16321 aaaaagtaag tgcgctaaat tattaaacac aacgacagca ataattgaat tttctagtct
    16381 atttataacc caagcagcaa gtttagcgct cacgctacct tcttctgctt tggctcttat
    16441 tttaaagcgg ttcgcagtca taaacgccgt ttcagatgaa ctaaagtaca tcccagcgac
    16501 aaaatataat attaagagaa cccacgtctg agggtcatca atcatttaga aaccacactc
    16561 cttctttata aatgtgcata ttcatatgca acgatagcat gttatcacag cgccgaaatt
    16621 tgtgcaacgc taaagttaac taagcaaggt tgggtcttga tcactattac tataaccttc
    16681 atcttcaata ataacccgtc caactttcgt cccgccctta taaaagcgca tcttccatgt
    16741 tgttttttcc acaactttat cgaagatata tagcaactgt ggtaataaca caagcacaag
    16801 gataccacta agtgctgcac cacggcctat tagttcacca agcgaggaca cgccttcaat
    16861 acttgagagg aattttagac tataaccagc agaggtcaaa attaagcacg atgttaaaat
    16921 actatgaccc ccgctatcaa gggcatactt aattgcatca cgtctaatca tcgtttttcg
    16981 tccctcagtg taaaatccag taaaaagtat agcgtaatca atcgtcgccc cgagttgtac
    17041 cgaacttaca atcatataac caataaaaat catcggttga ctcgttaaat acggaattgc
    17101 catattaatc caaatcgaaa tttgaataac aagcactaaa ataaatggca atgagatact
    17161 gcgaaaacta acaagtaata tgataaatac aaagccaatt gaaattaagt ctacaatcac
    17221 atagtcagtc tcgacaacat tacgaatttc tttaactgat ggcgagacac caataatatg
    17281 tgcatcacgg gcaaaaggtt gacgggcgac aacagtttca acaatatcaa cagcaatttc
    17341 cgcccgcggt gactcacttt cagtgttaac agtaataata atgcgtgaat aatgttcact
    17401 taaaaactgt gccttatatt cttcggtaat aagattatcc ataatatcgg caaaaatatc
    17461 atcaaggaaa tttttaatcg tttgatctaa atcatcgtag ttaggaatat aagtctcaat
    17521 atcacttaag tttgaaagac tcgtaatttg tacaacaaaa tcttgattag cgcgctcaaa
    17581 ctcgcgataa agctgtttaa tcatcgcttt ttcttgggca atataagcat ctgcttcatc
    17641 gcgtggtaca agaatagcca ttaagttttc tttaccaaac ttcgcttcaa tccgattttt
    17701 ctcaattgcc gctggtgtgc cttcgctcac actcatcgct gcttcaccat acacaaaact
    17761 attattagtt tgtgctaagt aagaaggcac aattacgagt aaaagtatcg caggcacaac
    17821 atagcgtaat tttacaatcc atttagtgag tccaccaaga agtgggaaaa agcgtttatg
    17881 ttttgttttt tccacaattt tatcagtgag caagattaaa ccaggcataa aggtaaaggt
    17941 tgagataata ctaagcaaaa tccctttaat catcaccgcc gcgaggtcaa tcccgattgt
    18001 gtaacgcata aacatcagtg ctaaaaatcc ggctgcagtt gttaacatgc tcgagttaat
    18061 tgacacaaat gacttgcgca ttgccatcac cattgcgtct tttttatcaa gtccctctgc
    18121 cctttctttt tgataagcat gcaagagaaa tatggaataa tccatcgaga ttgctaattg
    18181 caagagtgag gctgtcgaat tcgttaaaaa ggatacagac gggaaaaata agtttgtacc
    18241 catatttacc aaaacagcga ccccgacggc aattaaaaag ataataggct cggcccaact
    18301 tctggtaaac actaataaaa taaataagat gattgggaca acaattaccg ttacccgcaa
    18361 tacttccgat tcagttaact tctgcgtgta ataagcatta actgctgttc cgcgcattgc
    18421 ataaggtcgc ttgataccct caagatagga gcggatttgt tcaatggcct tgccgacagc
    18481 gcgactatga tcttcttccg taaacacaat ttgcagcaaa gcacttttat ctttataaaa
    18541 agcgttaagc cccggaatat ttgcgaagtc aaagacaccg gacatcccat tttcttcagc
    18601 aatgcgttga atatcattaa tcgtatcgcg ttcgacgaaa ctatcaatcc acacaacttc
    18661 cataaccgtt ggaatactta taatttcatc tttatacacg aacacttctt gtaatgtttc
    18721 aactttttca atcattacac tagcttgacc agtaatgcca aactcttctt ccatcacacg
    18781 taaagattct ttaacacgcg tatcttctgg caaatatttt gtcgtgtcgt aatttatttt
    18841 gacatgggga atcataaaaa tactagcgat tgttaacgct atccagaaac cgaaaaagat
    18901 tactttgaag cggacgatga aattaaatat ttttttcata atgcattaat cctatctttg
    18961 acatctttaa ataagaacac ataaagtaat tttaaattag atacaatatt ctctggagca
    19021 gattttcgca aaacaaaaac agtgccagtc gcgataaagt gataaagtag gtcaaacatt
    19081 ggcattgtaa tctgcggcaa gtgtgctttt accgtgcggt aaataagacc acgaaagttt
    19141 tgaataactg gatcatcttc ttgcgctaaa tagtattcaa gaaaaataac cgactccttg
    19201 tgatacttag aacatatcgc ggtgaaccgc gtcataatcg tcgttaaaca aagttcattg
    19261 aaatgcgctt ccggattatc gtgcgttgct tcaagtaact caacaagctt ttcatgtact
    19321 ggttgtacaa gcgtagtaaa taagtcatgt ttgcttttgt agtagcgata agtgttccca
    19381 acactcatcc ccactgccgc tgctaaatca cgcattaaag ttgcttgata cccgttcttg
    19441 taaaagagat caagcgcctc tttttggatt aatgaattaa gttctggttt taaaagttgc
    19501 ataatgtaaa cacctgttca ctaatattgt atagcaatgc taaaatcgtt gcaagaaaaa
    19561 aataaaaagc ccatcaaata tgggctttat aatgtaaata attaatattt tataagaaat
    19621 aaataacagt aacatcgagt agtcttgcat tagccgtaac gacaagtgaa agactagtag
    19681 tgttatcagc gtcaaagtga tataaaacag cgttagccgt tttaaattgt tgagcacctt
    19741 cgacaactgg gaaagcgtca attgacttaa catctacacc atcttctgta taattaacac
    19801 catggaaaat aacttgtttc attgttcctt gttccatatt taaagttaag acttgatctt
    19861 tataaatacg aagttgtggg gttaagtagc cgacaccact attattcgga atatttccaa
    19921 catttgtact tgattcacca acctcaactt taaacttgta aagatcgcta aatgcccatt
    19981 caacaatttc ttttgatctt gttgttactt taaaatcatt agagaaagtg atttcagagc
    20041 gatgcgcact acggtcaact tttggtgcgc cagggaattc ttctttttct tgaacagtaa
    20101 agcggaaata agtagcgctc ggaacagctt cattttcttc tacaaaacta acaccaatcc
    20161 gtactttacc gctgaaagct tcaataccaa attcgtgttc ttcatcaacg ataactgtgt
    20221 aatcggtttt accatcatag agcgcaacat aattagtaaa agttgtggca tcagcagtga
    20281 cgcgcactac ttcaaatgat ttaaaaacat caatcccaag ttcatcttca taactgcgaa
    20341 cataaagatc agtattttga ccaaatcctg ttggtgttaa aacattcgtg aatacccacg
    20401 ctgcatctat cccttcacga gtgtagtgca ttcttattga ttcaactggc cagcccttaa
    20461 gcggtgaata accagcagcg ctaaatcttt cagcttctgt aatttcgctt gtttcactac
    20521 cagaagtttg actgctcgat gtattaggat catcacatcc tgttaagagt aagccaatta
    20581 ctccaattgc taacataaat ttcttgagtt tcatacataa cctccgctat caagttatat
    20641 tatcatactt catttaatat tttaaatgat agagtaagaa agcggataca ttattacact
    20701 ttatattaaa aaagcctatg caagcatagg ctaatttatg tttatttact aattaatttt
    20761 atttaactag ttctttaacg tggtcaactt ctttcatgtc atgcttacgg gcaatgccgt
    20821 gtttaagcat tacatcatca attaattctt gagcacgttt aactgataag tcactgcgga
    20881 catataagaa gactggtaca tcttcatatt gcttaatacc ttcagcggat ttaagcggaa
    20941 tcttactatc tttatagtct tctaaatcaa gtggtaagta aagtttaagt ccactgtttc
    21001 cggagtttgt taagcgtgca tataggacac gacctaagcg gaatgaatcc gcgacattag
    21061 aaacacgtga atttaagcca taacttaata aatagttttt aagtaagtta tagttagctt
    21121 ttgcggaatc ttcaaccatt tgcatccgat ctgggaagga aacacgttca attttcggtt
    21181 ttgcttcagc ttttggctta gcaacgacga ccggttttgg ctctggtttc ggttctggct
    21241 ttggttcagg tttcggctct ggttttggtt caggtttaac ttccacaacc ttttcaactt
    21301 cgcgcaccac aacttctttt tctggaactg gacgcttgtc gatttcttca ttcgctaatt
    21361 tacgaattaa agcttcgagt ttcggatctt ctaaatcaag ctcaacaata agttcttctt
    21421 cctcttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntct tcaacttttt
    21541 cttcgacaac tggttcttct tctttttctt caacaacgcg ttcttcaact tgttcatcaa
    21601 ttacaacata ttcatcgtct aagtcttctt ctaataatga agcaactttc ttcgtcccag
    21661 cagttttaag accgacaact actaagtgga gtgaaagact aaccattaag acgccaacga
    21721 caaagaagta agcagttgca acttgcgctg ttggttgacg gaatgcaagg tcaacatcgg
    21781 tgtgacttcc aactaataat actcctaaaa agatagcaat aaggaatagg agtggcgata
    21841 ataagttcca accacgacga cgatagtaaa gtaatttaat cgcccaaaca agtgctaaaa
    21901 caagagcaac accaaggacg actactgtag cgacaaagcc cgctaaagac ataaggtcac
    21961 caaagtttgg aacaaataat gataatagta cttctttaaa tttcactaac attcctaaaa
    22021 caccaatcgg ggcaccatca accaagtata ctgtcgaaac aatatgcgct aatccgaaaa
    22081 aggttaacat taagattgca agaataagag taagtactgc actagtgcgt tttttctgca
    22141 taataaaatt gtcctttcat taattttaat tactttcata ataataaaaa cacacgcaaa
    22201 ttgcaataga aagtatgcaa aaatgatata ttttgttgaa aaatcagcta ttatagctta
    22261 ttttgcattg ctgtaagacg tttttcaact tcctttggac ctaagattga aagcacctca
    22321 tacaaattta ggctcttttt gcttagtact attgctagac gtaacacttc tgcacaatca
    22381 gcaacattac cattatataa ttctggattt gtgcgatatg ctttgttatt aatagcaaaa
    22441 ccatgctttt ctgctacttt tttaagcgaa tcaaaccaca cactattacc ctcaccatat
    22501 gtaagttcag ttttaaatga ctcaagtaaa ccaattagtg tgtctttcga taacgactct
    22561 ttaaattcac caatatcaaa atcttcatgc atagcggcga attcatcact aaagaaaaac
    22621 ttaatggaag gataaaccat tgaataattg tgataatctt ttcgcggttt ttcgccttcg
    22681 cgttcaatat tcaaaatctg cacaaataaa tcataattgt ctttaatgcg tttcactaat
    22741 ttttcatttt tattatatag agcgtatgcg agatattcat ccactacttc ttttgcagtt
    22801 aatgaagcaa tatattcacg ggcaaagaaa tttacttttt caatgtcgaa gagcgcacca
    22861 acaacactca tcttactaat gctaaagggg aattcactaa gtggtttttc acgattttct
    22921 gcccaccact cttcaaagtt tgaattagcg atgctcatca aataagtaag aacagcgtca
    22981 actggatatc ctaattctaa gaaatatgta acagcggctt ctggatcacg ccttttactt
    23041 aatttccgtt tattaccatc atctaattta tcgattgttg gtaagtgtgc ataacgcggt
    23101 tgattaaatt taactgctgc aaacatttct aaatgtaatg ggacactact aatccattct
    23161 tcaccacgtg agacgagtgt tgtacgcata aagtgatcat cacaaacatg ggcaaagtga
    23221 tatgtcggta atccgtcact ctttaaaata acgatatgtt gatcgttttc cgcaatttca
    23281 atcgtacctt ttacttcatc gtcaaatgaa acacgatttt catgatttcc tttgcttcta
    23341 aaacgaataa cataaggaac gcctgcttca attttagcga tcgcttcatc tgcacttaat
    23401 ttactacatg ttgcgtaatg tccgtaataa ccagagacta ctttactagc ttcttgtgtt
    23461 gcacgtaaaa gcgctaaatc atcagtagtg caaaaacaag ggtaagctaa atcgtcctta
    23521 atcatttgtt taattactgt tttataaata agagcacgtt cactttgttt atatgggccg
    23581 tagtctccta attcactatc acctaagtag ccttcatcag ggtatacttt aaatattttc
    23641 atttcattaa gtaaattttc accagcgcct gcaatttcac gttttgtatc agtgtcttca
    23701 agtcttacaa agaacacccc accactgcgg tgcgcaacag tgcgagaaac taaagccgca
    23761 aataaactac cagtgtgtaa aaatcctgta ggtgaggggg caaatcgtgt aacttcggca
    23821 ccatctttta aactacgatg cggatatctt tcttctaaat cggcaattgt tgtagtaatt
    23881 tctggaaata taatattcgc taatttttct ctgctcataa ttaacctctt ttaacacata
    23941 taaatatacc atactatgta tatttttcat caattttact aatttataag gacattaatt
    24001 gaataaattt cataagagtg ataatataat caagcacgca ggtgtagttc aatggtagaa
    24061 cactaccttg ccaaggtaga tacgcgggtt cgattcccgt cacctgctcc atctaactat
    24121 gaattagctt ccaaatcttg gagagaggta taatagaaat acccacccaa gaa
//
LOCUS       DAOSLP010000014        23434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_2414_length_23434_cov_7.426451, whole genome shotgun sequence.
ACCESSION   DAOSLP010000014 DAOSLP010000000
VERSION     DAOSLP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 23434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23434
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_23434_cov_7.426451"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1336)
                     /locus_tag="PK460_02285"
     CDS             complement(206..1336)
                     /locus_tag="PK460_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOE53955.1"
                     /translation="MREAYKKIPCRITVIDLLKLTGMIFICIPHCLQRWGPSDYTSTL
                     GFSFFYSVGLSMFFFSSGIVLRRITSLKDLLIFIVKTVGVYLTSAFIFTVLSIYTLPR
                     FTLLEKDFGYWMNELYLRTDTFYWYLLVATLINIFIASAYYFVSLIKKNNLKWDIFKA
                     SLIIILTVAYSMIFLHIYNREDLGPGCLGASLVLYYLPITLFGFLFNIFKPYLLKLPR
                     QELLKGIATLVALVIYIPLLIVYRDWLPHLNGSFWTISALWICSLSGTIVYYYLLSKI
                     SHFTIAKKVSCYGFFSGQFYLVHVYIIRLFATYIPRPETFDFPAIMFVTFLTIIFTIG
                     SILITVFLRFIPYTDFLLFISYDRYKDIIYPHRWLKRVVLQK"
     gene            1474..2481
                     /locus_tag="PK460_02290"
     CDS             1474..2481
                     /locus_tag="PK460_02290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE53956.1"
                     /translation="MRILLFNKKQKLYDKSGVGRAMKHQVKALTEAKIPFTTNEKDDY
                     DLVVINTIDPSAFRMARKARRQGKKVIVYGHSTKEDFMNSFMLSNQIAPFFKIHLKRI
                     YKMGDLVITPTPYSKKLLDTYGLNREVVALSNGVDLARFNADNKEKIQKFREYFKLKP
                     DQKVVMTVGLFLARKGLHDLIEIAKDRPEYTFIWFGHTPKAVLSRKIKKAIKNKPDNV
                     IMPGYIAGDIIEGAFANADVFFFPTYEETEGIVVLEALASKCPLLIRDIPIYEEWLTH
                     GVNCHKGKTNEEFKALLDDIITKKAADTREAGYKVAAERDIKEIGKKLGEIYRRVLEE
                     K"
     gene            complement(2507..3349)
                     /locus_tag="PK460_02295"
     CDS             complement(2507..3349)
                     /locus_tag="PK460_02295"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HOE53957.1"
                     /translation="MNDLQKVLLENLKEFIRVCEKHNLRYYLVTGSALGAVRHKGFIP
                     WDDDIDVAMPRNDAMKLLALKDEFKEPYFLQHYTTDPSFPYPFMKLRNSATTFEEGLL
                     KFHKINHGIYIDIFIIDGMSKTSTLKNKWKNALMWPVMYLIYAANFWQKPRKNTFILQ
                     ILLYLVSILLLPFKINNWLIKALDKSMAKIPFEEATLVGAYLLWGGNKEAMDKRIYGP
                     GVKMKFETIEAVLPENYDAYLINRYGNYMELPPEHKRVGHHKPSALSTKVSYKDYYKR
                     KKRR"
     gene            complement(3370..4257)
                     /locus_tag="PK460_02300"
     CDS             complement(3370..4257)
                     /locus_tag="PK460_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HOE53958.1"
                     /translation="MNKKQLEIMRSGKGFIAALDQSGGSTPKALRTYGVPETAYKTDE
                     EMFTKVHEMRTRIIKSDAFNSERILGAILFENTMDRKIDASYTADYLWEEKGIVPFLK
                     IDQGLCEPISGVQLMKPMPNLASLLERAKERKIFGTKMRSVIKEANEQGIKLIVEQQF
                     AVAKKVINYGLVPIIEPEVDINSPTKKEAETILLKFILEELAKLGPNDLVMFKVTLPT
                     MMNLYAPLLKDPRVVRVVALSGGYTLEHANDLLYLNKGVIASFSRALVADLRVDQSDK
                     DFDKTLAKAVESIYEASLT"
     gene            complement(4348..4959)
                     /locus_tag="PK460_02305"
     CDS             complement(4348..4959)
                     /locus_tag="PK460_02305"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOE53959.1"
                     /translation="MVRKEIKQQAKDLIKNGKWFLLFVVLLIVSAVSSLTAGILMPVA
                     LFGLHLAIVGMFAGQEFDFNHFGTPFNDFNHALKVIAVYFLTMLIVMVGTMLFIIPGI
                     IFYMMYVQAYRIMIEKPEIGIMDALRESKEMMNGYKMDYFVFQLSFIGHFLLIMITFG
                     LWGIYLMPYFVVADTNYYLHLKQVRNEAKVVAEAAPVEAEVVE"
     gene            5127..6287
                     /locus_tag="PK460_02310"
     CDS             5127..6287
                     /locus_tag="PK460_02310"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOE53960.1"
                     /translation="MTIKDHIKHRFAAIYNTPPVAVYFSPGRVNLIGEHIDYHGGLVL
                     PVALSIGTYGAYSLRDDDEVRLYSTGYSDYPITINLGDLSKDSFTGWANYVRGVFQVL
                     KDEGYHVPHGLNLYLESDMPTSGGLSSSSSLELLIIKMLNDIFNFGIDRTKMAVLGKK
                     VENEYIGVLTGIMDQFVIANGKKNNALLLNTNTLKFDYIPLDLKDYQLVIVNTNKRRG
                     LADSKYNERWNETMGALKTLKNHFKINNLTELKSSDLPQIEKLLDPLLFRRVRHIITE
                     QERTLESAMLLKEGKIEEFAKLLTASHNSLRDDYEVTGLELDTIVLLLLETGAIGARM
                     TGAGFGGCAIAIVPKEKVEKQAPLVIDLYKKVIGYAPSFFASDSSDGTHELK"
     gene            complement(6279..7241)
                     /locus_tag="PK460_02315"
     CDS             complement(6279..7241)
                     /locus_tag="PK460_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53961.1"
                     /translation="MKLYVLENDLLKVTFLDVGASIYSFSVKALDNRNIVLTTKDKNI
                     YKEPPKNAYFGATVGRVGGRIANGEFIIDGKKYQAAQNEKVTNSLHGGFESYAAKLFE
                     VVEEKEDYITFRYVSRDGEGGYPGELELYVKYTLLDNCLALDYEARTTKKTLVNIINH
                     SYFNLNGKGDILKHKVSADVEAVFALDDKQINLLPLKVRKGDTFDLSTGKKLEKMVFA
                     KEVNVPPMGGVDHLLVTPKRKLTLEGEDLRLIVLSDYPAVQIYSTNYPPEVTLLEGTK
                     VIKYGGLAIEPADIVCGQNGEFKNLELCPGEVYKRRIKYIVELL"
     gene            complement(7485..9278)
                     /locus_tag="PK460_02320"
     CDS             complement(7485..9278)
                     /locus_tag="PK460_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53962.1"
                     /translation="MKKRTSKLIMLLLGLSLLAGCFKTPSSEKPPVSSEQPPVTSVTT
                     EEPPVSSEQPSEPPVSSEEPPSSEVEPGEPIKLGDAAAALADPNEIYLQVGEKVNLTY
                     ARIVDNVPRIQFTVEDGVAYDALKLFVKFPGEVGKRYKVNAAFTAAAAFTGTINGERF
                     DFIRGENNLEFYYDELENEVSIAFVFGFNEEGLGLKANNITFGEGSISERVYDDPETT
                     LAVDGDISDWSNLKAFENTVGVHSNTAEVKHKSVDFYASLTSTGLYLAAEAYHDILIE
                     ESEHWWQSTNFEIFIQGNNQYWVSARKVDGAFTKRDNVTEAAMVTTPLTGEGVANFRT
                     VAEMFIANANLPEAAVVGGEIRVGFAWKTDGDLLDNGEAAGGGLDCYWVPKGTWTDNA
                     DQTFVTINGIFKQTQLTFEPTTMTIDGDLTDWEEKAAYTTNKIYIEGSDATNHKNVTF
                     MAFLNDEGLFLAALAHHDVLINNEEIWHSNTNFEFFLNGGNQYYVTAAGQVSYGKGII
                     VTQAYKGEPYAEYESVAEFFIPRWALPEGDMHRVGFAWKTNGDTATGLGGSGGGPDAW
                     WFEAGHFPNNADEQYYVTAEGLFRTDPTPAP"
     gene            complement(9373..11109)
                     /locus_tag="PK460_02325"
     CDS             complement(9373..11109)
                     /locus_tag="PK460_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE53963.1"
                     /translation="MYRKEYPRPALVRKEWLNLNGIWDFAFDDDNVGLNENWVEKGKF
                     PLKINVPFAFQTPLSGINDPSFHDYVWYRRFFSVKKEEGKRFILHFGAVDYECKIYLN
                     KKHIGDHIGGQVPFSFDVTDFLNFNEEELVVWVSDPSTELSMPRGKQYWKEKSEVIWY
                     NRTTGIWQTVWLEIVPEAYVKSMRITPLFDEGKVHFSLSFSQVGRELILEDENGKIKY
                     AKVPNKLTHEFTVQTFLDEQDFITKAWSPENPHLFPFKLRYGDDLISSYFGMRKVHAD
                     KGITYLNNKPYYMKLVLDQGYWPQSLVTPPSKEALEYDIKMAKAMGFNGARKHQKCED
                     PYFAYYADKLGFLVWGEMASSIRFSEKSAWRDKHEWPQIIARDYNHPSIVAWVPLNES
                     WGVDDIGHDKRQQQHALELYYLIKSLDNTRFVVGNDGWEQIITDICAIHNYNHGRHDD
                     KDAIAAFNKSISTKEELLKSRPANRDIYAEGYSYRGEPILLTEFGGISYQMNEDKGWG
                     YTAVGDAEHLLSEYKRIILAIKESTALVGYCYTQLTDVEQEINGLLTYDRKPKLPVEK
                     VKEVNDLIQKYK"
     gene            complement(11127..11993)
                     /locus_tag="PK460_02330"
     CDS             complement(11127..11993)
                     /locus_tag="PK460_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE53964.1"
                     /translation="MAIKSGVKRRKRVAFIILLVLSILWVLPLLWALGTSFKSANDMR
                     TNVITIIPREFTFGNYKNLFDNSAVYPIFKWLRNSALIAIIHTLLYLIIASMAAYAFG
                     VMRWKGRNIVFGFLLATMAIPTVINLTPLFTMVIRFGWLDSEAAWKSIFALIVPGLGG
                     TFGLFIMRQFFMNIPYDLVESAQIEGLGHFGVYRKIILPLGKSAVMVAGLFAFMGSWN
                     DYLWPTLTAAVHFDQNWYTLQTGLATMQSQSNYDPGQVMAAAIISIIPVLIVYMFVQD
                     KIIEGVSHTGIK"
     gene            complement(11993..12964)
                     /locus_tag="PK460_02335"
     CDS             complement(11993..12964)
                     /locus_tag="PK460_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE53965.1"
                     /translation="MDAKQKLKLKEGIKRYLTVAFFLLPYIALFATFFIFPLFFGVYI
                     SFFRYNVFDPSASKFIGFDNYKLAISGLTIGTTNVTYYAKMFWGSLKNTLLFVCVSVP
                     LLIIIPIFIAILIDIQPRFYKVFRVIFFLPTILSISAVGIIFKWQFDTQYGFVNGLIR
                     VFGGKEVAWLNSQPYAWIAIVVTTIWWTIGTNTVIIGAGLKEVDKQMYEAAEVDGASY
                     PRVIWSIALPSIKNQIFIIVFTTIIASFNIYGQPDILTGGGPAPSGTVAAGTPGTTTV
                     VMMVIRGLMTGVNRQPGLAAAMSLIFGSFILVVSLGQAAYNKYREKY"
     gene            complement(12984..14309)
                     /locus_tag="PK460_02340"
     CDS             complement(12984..14309)
                     /locus_tag="PK460_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE53966.1"
                     /translation="MKKKTIPLLLLALVLTGCGQTSEKPSQPGGGGEGSVEVSLPTFE
                     NVALTYGNPITGADGTAMRRLVREFNEEYQGQITVTESFMPEVDFYESLVATIPMKRS
                     FDIALIHSYRVPSFANKNLLFPLDNLTASTGVDITRENYIEDVYDAMIWDNKQYGVPL
                     DVHSIMLYYNKDLLAQHGCEVPTNRAELIAAAKKMPQTNAGGWGLPLSTTWPSEYIFT
                     TALYQNGGKEIDKNTLMPAFNNTAGEKALKAVADLIHIEKISPLNVNVDADLLMFQQG
                     KAMFHINGNWMLNSLVDSGINFGVTSLADMFVDTPTADSKKVASRSHTFVLPQGRNET
                     IKQQAALVFIKYVTENAYLWAESGGHIPASNIARNTEEYLALPYHQTYGDVNMYTLNE
                     PSPYYYEAFAPVFSRVTTAMSDATYDAMALLNAAAEEGEQAVLIAQLEG"
     gene            complement(14338..15483)
                     /locus_tag="PK460_02345"
     CDS             complement(14338..15483)
                     /locus_tag="PK460_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOE53967.1"
                     /translation="MKRKIGFTLILCTLLVGCTPPVSSETLPPTSEDVVTSQSLEEVP
                     TTYENPVWEPVLADPAVIRADDGTYYAYGTQDNALWGDYFGVRYTPILSSTNLVDWEY
                     RDQAFKPATMPTWSETSGAGLWAPDIVKIGNTYNLYYSLSAWGDPNPGIGVATGPSPL
                     GPFTDQGKIFDSNEIGVYNSIDQAVFVDTDNRVYMFWGSFSGIYAVELTADGLSVKGT
                     IAEANANKVLVAGVDNKRWDIGNYEGAYIIRERGYYYMFLSTGTCCEGHHSTYEVRVC
                     RSESILGPYYDKAGNLMTYRQNLGTLVVRGNDDFAGVGHNSIVKDDAGESYIVYHGFD
                     KFEDPYYGTTNRRALLIDKLVWDKEYWPSVEGLGPSRGAERPYIAPR"
     gene            complement(15483..17501)
                     /locus_tag="PK460_02350"
     CDS             complement(15483..17501)
                     /locus_tag="PK460_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOE53968.1"
                     /translation="MKKHRYLVLALLSLVLLGSCKGDGSESGKTSEPQVSTTSTVGSE
                     EGERTITWYGTDDIVITIGDEFTLLDGVRAVDSVDGELEVVVADDDYFDCYYVSQYTI
                     KYEATNSVGTKSTKYRMVQVVKGCNVFNGNFAMGKTYWRFDTPGGQGTFNVINEEAVF
                     SFTDTGSEAWSLQLYQQPGITFSAGRTYEMTFIAKSDKGRSISAGFENYNGFAMLIPG
                     YPAMVLTSEFQTYSAIYTADADYPSVKPVLYLGRGLDIDGTASKANPLDLVVDDIKVR
                     EIVLPEESKRPVFSNAGPVTVFTGDQFEALPPVTAVDYKGNDISDRIERIGAIPDSIA
                     AVTRMLVSYRVTDTEGNFNFINRSVSYQIARDNPYNLINGDFNNGLQGWVRDVNQTNG
                     TGAANFIDNEDGTITIDITNGSNDNWHIQFYQSNVSIQAGKIYRVTLIAKASIERKAT
                     IEISNPSQSYSRVATKLISLTTEYQTFLLEFKSTVTCLAKVSLLLGAQGANQVTLDKY
                     DNDMINADEATTIDLRDYEDYEVINGDFRYGYYGWTQEMTHGAEVDFIADQANEQIKI
                     DVISPGTANWHVQINQDGKTFTEGKTYIMKVSASALAATTIAMEVTNNNGETVLARED
                     ITLSATLTEYTMEFTPSQTFTLGKCALLLGTSEVTVITVKSVAIGIKP"
     gene            complement(17733..18872)
                     /locus_tag="PK460_02355"
     CDS             complement(17733..18872)
                     /locus_tag="PK460_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOE53969.1"
                     /translation="MKRYRILLPLLCVALLVSCNQTSSREEPITGSEVVSEISDDSLS
                     ETIASSEKPRELTTVFFQNPIYNADFPDPSIVRHTDGQFYTFATGGRIIKSANLVEWE
                     ALSNAIARPTWGTPGANIWAPDVQYINGQYVMYYSLSRWDDPNPGIGIATATHPAGPW
                     TDHGKFFLSQEIGVNNSIDPMVFVDEDERVYMFWGSFRGIYAIELTADGLGFKDGSVE
                     QAANNKVHIAGFETNRNFDISTFEGVYIIKKDNYYYMFLSTGTCCSGDYTYNVVVGRS
                     ESILGEYFDSYNRSMKNANVGHSVVHQNDFFIGVGHNSILRDDAGTYWILYHGFDATV
                     ETRNARKLLIDKLIWNADNWPSTLGNVPSNNYRPGPELYLSDENN"
     gene            complement(18886..20133)
                     /locus_tag="PK460_02360"
     CDS             complement(18886..20133)
                     /locus_tag="PK460_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOE53970.1"
                     /translation="MKIKRHVRHLMVLVFMMVISSCALFPTSSEKDSLPIASSSSETF
                     SSANESSEELSTSEVPSSESEVQSSEPEPSSKELSSSEEPSISVELEMKRSTYTNSVY
                     FYDFPDPSGVYDPIQRMWFLYATGGQYLRSSDGVTWGQKGWVFGHLPDWGTKGAGLWA
                     PDVQYINGQYVMYYSLSTWGDPNPGIGYATAPKPNGPWTDHGKLFLSQEIGVNNSIDA
                     NAFVDPNTNRVYLVWGSMRGNYLVELTRDGLGFKAGSVEQAALSKIRVAGLDTVHGWD
                     AETYEAAYIRYWDGYYYLFLSMGTCCEGLSSTYKVVVGRALSPYGPYYDDTGTDMRNR
                     SAGKLVLQKNDTFVGTGHNSVIDDVNGEPWIYYHAYHVDKQSARVLLQDKLLFDENGW
                     PYVEGFTPSTTEQIGPYYHSVVE"
     gene            20269..21291
                     /locus_tag="PK460_02365"
     CDS             20269..21291
                     /locus_tag="PK460_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE53971.1"
                     /translation="MEKNNKERVTLKDIAERAGVTVNSVSRALRGHKSISEPTKERVR
                     AIAKELGYIPDAQARLMRGAKLRVIAVVYDNIVNPYYAIMNNHLNNELTKANYRPMVF
                     FESSQDGSLSLEVAREIISMRIVGVITYLVPSKEVIELFEDHNVPIVLLGGTGDALGI
                     DSVASNDYRGGKLAGYKLFDLGGKNFAYIGVTRKLNINNERLNGFLKALKNNDVIVPE
                     ANIILNEKQQTTRQLMDTLDVQKKKIDSIFCFNDQIAYEVIGYLSEKGIKVPEDINII
                     GFDNLQSAIIYPIKLTTIDADKPRAAHLALVSLFSKIKDSEDTEVTGKIVDVTLVEGN
                     TTKMRK"
     gene            complement(21378..21545)
                     /locus_tag="PK460_02370"
     CDS             complement(21378..21545)
                     /locus_tag="PK460_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53972.1"
                     /translation="MSVVLLALLKNFAVMPNVDEPAKALEAYKLTGGAVVALVLAGLG
                     GVAGVAADLLK"
     gene            22287..23243
                     /locus_tag="PK460_02375"
     CDS             22287..23243
                     /locus_tag="PK460_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOE53973.1"
                     /translation="MLTRVIGLDIGAATMRAAVVDREGTIYYSKEVATPERSELRFVR
                     EINDLLENVLFHAPEAIAIGVGVPGIVSPGTTLIHLLPNLGYENIDFKRILRKEIKQP
                     VYLLNDANAACYAEALFGAGADKRIVQYITLSTGVGGGLVINKEIYTGARGFALEIGN
                     MIIDTETPRPNDKMNRNSFEGSCSGKSLYNQAKRFGTNIHTVASVFNESRFSPIRNVW
                     VDNLGKGIANIYSLYDPDVVILGGAVMQSADKFFDDILPAVEKYTLPQLHPYINLKRS
                     HFGETGGMIGAALYALHEHEKIINKAKSDEVKIDTEVKAETK"
     gene            complement(23244..>23434)
                     /locus_tag="PK460_02380"
     CDS             complement(23244..>23434)
                     /locus_tag="PK460_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53974.1"
                     /translation="FSFNGGTGFKAKVLFYIFPFLYLSGIATPIKLEWLWATLTNVGY
                     LALLTTLGILLANRTDYK"
BASE COUNT         7461 a         4738 c         3984 g         7251 t
ORIGIN      
        1 tctattttta aaactttatt ttctattttg tcactttatc tttttcatgt aacaaaaatg
       61 ttttagaatt acactttttg taatttatga ttatttattt tgcaaagtaa aataaacaca
      121 gcaaattttt attcacggtt gaaacatttt ttcattatta attgacgact atcagcgtag
      181 cacttcttaa ttaaatcatt aaattttatt tttggagcac tactcttttt agccaacgat
      241 gcggataaat aatgtcttta tagcgatcat aactaataaa taataagaag tccgtgtaag
      301 gaataaagcg taagaaaaca gtaattaaaa tactaccgat tgtgaagata attgttaaaa
      361 aggtaacaaa cataattgcc ggaaagtcaa acgtctcggg tcgcggaata tatgtagcaa
      421 aaagacgaat aatatacaca tgcactaaat aaaattgtcc gctaaagaag ccgtaacatg
      481 atactttttt agcaattgta aagtgtgaga ttttggaaag taagtaataa tacacaattg
      541 tcccgctaag tgaacatatc cataaggcac taattgtcca aaatgagccg tttaaatgag
      601 ggagccaatc gcgatacaca attaaaagcg gtatataaat tactagcgca actaaagtag
      661 cgataccctt aagtaattct tggcgtggta gtttaagtaa atacggttta aatatgttaa
      721 aaagaaaacc aaataaagtt atcggtaaat agtaaagcac taaagaagca cctaagcacc
      781 ctggtcctaa gtcttctcta ttataaatat gtaaaaaaat cattgagtaa gcaactgtaa
      841 gtataattat aagacttgcc ttaaaaatgt cccacttaag gttatttttc tttattaaag
      901 acacaaagta atatgcacta gcgataaaaa tattaattaa tgttgcgaca agcaaatacc
      961 aataaaatgt atcagtgcgt aaatacaatt cattcatcca gtagccaaag tctttttcta
     1021 aaagcgtaaa gcgcggcaaa gtataaattg ataaaacagt aaaaataaag gcggatgtta
     1081 aatacacacc aacagttttt actataaata taagcaaatc tttaagtgat gtgatgcggc
     1141 ggagcacaat tccgctacta aagaaaaaca tcgaaagacc aacactatag aaaaaagaaa
     1201 agcctaatgt tgatgtataa tcacttggcc cccatctttg taaacagtgc gggatacaaa
     1261 taaaaatcat ccctgtaagt tttaataagt caatgaccgt tatccgacac ggtatctttt
     1321 tgtaagcttc acgcatagct ttaactcttt ctattattaa gggtttagtt ccctttaata
     1381 gcaataatta tagcattaaa ttataaacta ttaacacgtt caaaaaagta tatatcgtga
     1441 tatgataaaa agagagtgaa aggagcccgg agtatgcgaa ttctcttatt taataaaaaa
     1501 caaaaattat atgataagtc tggtgtgggt cgcgcgatga agcatcaagt caaagcatta
     1561 acagaagcaa aaatcccgtt taccacaaat gaaaaagatg actatgatct cgtcgtcatt
     1621 aacaccattg accctagtgc tttccgtatg gcacgcaaag caagaagaca aggaaaaaaa
     1681 gtaatagttt atggtcactc aacaaaagaa gactttatga actccttcat gctaagtaat
     1741 caaattgcac cttttttcaa aatacactta aaacgcattt acaaaatggg tgaccttgtc
     1801 ataacaccaa caccatactc caaaaaatta cttgatacat acggcttaaa tcgtgaagtt
     1861 gttgccttat caaacggtgt cgatttagcg agatttaacg ctgataataa agaaaaaatt
     1921 caaaagtttc gtgaatattt taagcttaaa cctgaccaaa aagtcgtaat gactgttggg
     1981 cttttcttag cgcgtaaagg tctccacgac ttaattgaaa ttgcaaaaga tcggcctgaa
     2041 tatacattta tttggtttgg acatacccct aaagcggttc taagtcgtaa aattaaaaaa
     2101 gccattaaaa ataaacccga taatgtaatt atgccaggct acattgctgg tgatattatc
     2161 gaaggcgctt ttgcaaacgc tgatgtcttc ttcttcccaa cttacgaaga aacagaagga
     2221 attgttgtcc ttgaagccct cgcaagtaag tgtccacttc tcatccgtga tatcccgatt
     2281 tacgaagaat ggttaacgca cggtgtcaat tgtcataaag gtaaaactaa tgaagaattt
     2341 aaagcgttac ttgatgacat tattactaaa aaagccgcgg atacaaggga agcaggttac
     2401 aaagtagcag cagaacgtga cattaaagaa atcggtaaaa aactcggaga aatttatcgc
     2461 cgagtactcg aagaaaaata gttaactaaa cgacaaaaaa ggcgccctag cggcgctttt
     2521 ttcgtttgta ataatcttta taactaactt tagtgcttaa ggcgctcggt ttatggtgac
     2581 cgactctttt atgttctggt ggtaattcca tgtaattacc ataacgattt attaaataag
     2641 catcataatt ttcagggaga acggcttcaa ttgtttcaaa tttcatctta acacctggac
     2701 cataaatccg tttatccatc gcctctttat tcccgcccca aagcaggtaa gcaccaacga
     2761 gtgtcgcctc ttcaaaaggg attttagcca tacttttatc aagtgcttta atgagccaat
     2821 tattaatttt aaatggtaat aaaagaattg atacaagata gagtaagatt tgaaggataa
     2881 atgtattttt acgcggtttt tgccagaaat tcgcggcata aataagatac atcactggcc
     2941 acattaaagc gtttttccat ttatttttaa gggttgatgt cttgctcatc ccatcaataa
     3001 taaaaatatc aatataaata ccgtgattga tcttatgaaa ctttaaaaga ccttcttcaa
     3061 aagttgtcgc gctattgcgt aacttcataa acggataagg aaaactgggg tcagttgtgt
     3121 agtgctgtaa gaagtatggt tctttaaatt catcttttag cgcgagaagt ttcatcgcat
     3181 catttcgtgg catcgctaca tcgatatcat catcccacgg aataaagcct ttatgtctta
     3241 cggcacctag tgcactccct gttactaagt aatagcgtaa attatgtttt tcacaaacac
     3301 gaataaactc ttttagattt tctaataata ctttttgtaa atcattcatc cttaatgtct
     3361 tttccttatt taggtaagcg aagcttcgta aattgattca acggccttcg ctaaagtttt
     3421 gtcaaagtct ttatcacttt ggtcgacgcg taaatccgcg actaatgccc gtgagaaact
     3481 agcaattaca cctttattta aataaagtaa atcattagcg tgctcaagag tataaccccc
     3541 gcttaaagct acaacacgaa caacacgcgg atcttttaaa agtggtgcat ataaattcat
     3601 cattgtaggt agagtcactt taaacatcac taaatcattt ggtcctaatt ttgctaactc
     3661 ttcgaggata aattttagaa gaatcgtttc tgcttctttt ttcgttggac tattaatatc
     3721 cacttcaggt tcaataattg gcactaaacc gtaatttatt acttttttag cgaccgcaaa
     3781 ttgttgttcg acaataagtt taatgccttg ttcattcgct tctttaatga ccgaacgcat
     3841 tttagtacca aatatttttc gttctttcgc tctttctaaa agactagcaa gattcggcat
     3901 tggcttcatc agttggacac cacttatagg ttcacaaaga ccttgatcaa ttttaagaaa
     3961 aggaacaata cctttttctt cccataagta atcagcggta taacttgcat caatcttccg
     4021 gtccattgta ttttcaaata agattgcccc taaaattcgc tcactattaa aggcatcgct
     4081 ttttataatt cgcgtccgca tttcgtggac cttagtaaac atttcttcgt cagttttata
     4141 tgcggtttca gggacaccat aggtgcgtaa tgcttttggt gttgaacctc cgctttgatc
     4201 aagagcggca atgaaccctt tcccgcttct cataatttct aattgttttt tattcataat
     4261 aaaaactccc ctctatctat acatattgta aaggaaaatt attaaattac atatgtttta
     4321 gcacaggaga gttttaattt ttaaaaatta ttcgactact tcagcttcaa ctggtgcagc
     4381 ttcggcgacg acttttgctt cattacgaac ttgtttaagg tgtaagtaat aattagtgtc
     4441 ggcaacaaca aagtatggca ttaaataaat tccccatagc ccaaaggtaa tcattattaa
     4501 taagaagtga ccgatgaaac taagttggaa cacaaaataa tccattttgt agccattcat
     4561 catttcttta ctttcacgaa gcgcatccat aataccaatt tctggttttt caatcatgat
     4621 acgataggct tggacataca tcatatagaa gatgatgcca ggaatgataa ataacattgt
     4681 acctaccatc acgatgagca ttgttaagaa ataaaccgcg ataactttta atgcgtggtt
     4741 aaaatcatta aatggtgtgc cgaaatgatt aaagtcaaat tcttgaccgg caaacatacc
     4801 tacaattgct aagtgtaatc caaagagtgc gactggcatt aaaataccag cggttaaact
     4861 tgaaactgca ctaacaatta agagaacgac aaagagtaaa aaccatttcc cgttcttaat
     4921 taggtctttt gcttgttgtt taatttcttt tcttaccata aattatcctc cttcttaaat
     4981 tataacctat ccaataattt aacagtagtt aaaattcaat tttttgtgat tacttgataa
     5041 ttattactcc tagagtaatt aaaaatgaaa tagggtaaaa tgtatttgat atatacttaa
     5101 aatctcatca caaaggagac aaacttatga caattaaaga tcatattaaa catcgctttg
     5161 ccgctattta taacactcca ccagttgcgg tatatttttc accaggaaga gttaatttaa
     5221 ttggtgaaca tattgattat catggcgggc ttgtacttcc tgtagcactc tcaattggga
     5281 cgtacggggc atatagcttg cgtgatgacg acgaagttcg cctttattca actggctata
     5341 gtgattaccc aatcacaatt aatcttgggg acttatcaaa agactccttt actggttggg
     5401 caaattatgt gcgcggtgtg tttcaagttt taaaagatga aggttatcat gtgccacacg
     5461 gtcttaatct ctatctagaa agtgacatgc ccacaagtgg cggactatca tcttcttcat
     5521 cacttgaact gcttattatt aaaatgctta atgatatatt taatttcggt attgatcgca
     5581 caaaaatggc cgtgttagga aaaaaagttg aaaacgaata cattggtgtt ttaacaggga
     5641 ttatggacca gttcgttatc gctaacggga aaaagaataa tgcactgctc cttaacacaa
     5701 acacgctaaa gtttgattat atcccgcttg acctcaaaga ttatcaactt gtcattgtta
     5761 atacgaataa gcggcgcggt ttagcggatt ctaaatacaa tgaacgctgg aatgaaacaa
     5821 tgggagcact taaaacatta aagaatcatt ttaaaataaa taatttaaca gaacttaaga
     5881 gcagtgattt accacaaatt gaaaaactgc ttgacccgct tctatttcgc agagttcgcc
     5941 atattattac cgaacaagaa cggacactag aaagcgctat gcttttgaaa gaggggaaaa
     6001 ttgaagaatt tgccaagctt ttaaccgctt cacataattc gttacgcgat gattacgaag
     6061 taacagggct tgaacttgat accattgttc ttctcctttt agaaacagga gcaatcggag
     6121 ccaggatgac tggtgccggc tttggcggat gcgcaattgc gattgtgcca aaagaaaaag
     6181 tggagaagca agctccactt gtcattgatt tatataagaa agttataggt tatgcaccgt
     6241 cattcttcgc tagtgatagt agtgatggca ctcatgaatt aaagtaattc aacaatatat
     6301 ttaatacggc gcttataaac ttcacctgga catagttcca ggtttttaaa ttcaccattt
     6361 tgcccgcaga caatatccgc tggttcaatt gctaacccgc catattttat aacttttgtg
     6421 ccttcaagta gtgttacttc cggcggatag tttgtactat aaatttgcac tgctggataa
     6481 tcactaagaa ctattaagcg taagtcttca ccttcaagcg ttaatttccg ctttggagta
     6541 acaagtaaat ggtcaacccc gcccatcggt ggtacattta cttctttggc aaacaccatt
     6601 ttttctaact ttttaccagt gcttaagtca aaagtgtcgc cttttcttac tttaagaggc
     6661 agtaagttaa tttgtttatc gtcaagcgca aatactgctt caacatcagc gctaacctta
     6721 tgttttaaaa tgtcaccttt accatttaaa ttaaagtaac tatgattaat aatgttcact
     6781 aatgttttct ttgttgtccg tgcttcgtaa tcaagcgcta aacagttatc tagtaatgtg
     6841 tatttaacat atagttcaag ttcaccagga taaccccctt caccgtccct acttacataa
     6901 cggaaagtaa tataatcttc cttttcctcc actacttcaa aaagtttagc ggcataactt
     6961 tcaaatccgc cgtgtagtga attagttact ttttcatttt gcgcggcttg atacttcttg
     7021 ccatcaatta taaattcacc gttagcaatc ctgccaccaa cccgcccaac agtagcgcca
     7081 aaataagcat tcttcggtgg ttctttataa atgtttttgt cttttgttgt taagacaata
     7141 ttgcggttat ctagcgcctt aacactaaag gaataaatac tagcaccgac atctaaaaaa
     7201 gttactttaa gcaagtcatt ttctaaaaca tataatttca taaaattatc cttttccatt
     7261 gtataaatac attttaatta tacataaaat aagtatgttt tgcagtaata aatgctgatt
     7321 ttgccactaa ataatagaaa aggtcctatt aatgatataa ctaggaccaa attcttaatt
     7381 acgagcattt tgtaaacaag gtctcctcgc acggtgtgtg cgaggagata gagataataa
     7441 tgaaattatc tgagtattat ttatgtctaa actttattgt ttaattatgg agcaggtgtt
     7501 ggatcagttc taaagagacc ttcagcggta acatagtatt gttcgtcagc attatttggg
     7561 aagtgtccag cttcaaacca ccacgcatct ggtccaccac cactgccacc taaaccagtt
     7621 gccgtgtcgc cattagtctt ccacgcaaag ccaacgcggt gcatatcacc ttctgggagt
     7681 gcccagcgcg gaataaagaa ttcggccact gattcatatt ctgcatacgg ttcgcctttg
     7741 tatgcttgtg tcacaataat acctttacca tagcttactt gccctgctgc ggtaacataa
     7801 tactgatttc caccattaag gaagaattca aagttcgtat tagagtgcca tatttcttca
     7861 ttattaatta agacatcatg gtgcgctaaa gcggcaagga agagtccttc atcatttaag
     7921 aaagccatga atgtaacatt cttatggttt gttgcatcac taccttcaat ataaattttg
     7981 ttagtagtgt aagcagcttt ttcttcccaa tcagttaagt caccatcaat tgtcatggtc
     8041 gttggttcaa atgttaattg tgtttgcttg aaaatcccgt taatcgtcac gaatgtttgg
     8101 tcagcattat ccgtccaagt accttttggt acccagtaac agtcaagacc accaccagca
     8161 gcttcaccgt tatctaataa gtcaccatct gtcttccaag caaaaccaac acggatttca
     8221 ccaccaacaa ccgcggcttc tggtaagtta gcgttagcaa taaacatttc agcaactgta
     8281 cggaaattag cgacaccttc gcccgttaat ggggttgtca ccattgcggc ttctgtaaca
     8341 ttatcacgtt tagtgaatgc accatcaact ttgcgcgcac taacccagta ttggttatta
     8401 ccctgaataa agatttcaaa gttagtgctt tgccaccagt gttcactttc ttcaatgaga
     8461 atatcgtgat atgcttcggc ggctaagtat aacccagtgc ttgtcaagct tgcatagaag
     8521 tcgacactct tgtgcttgac ttctgcggtg ttactatgga caccaacggt gttttcaaaa
     8581 gctttgagat tactccaatc gctgatatca ccatcaacag caagcgttgt ttctggatca
     8641 tcataaacgc gttcggaaat actaccttcg ccaaaagtaa tattgttggc ttttaggcct
     8701 aaaccttctt cattaaagcc aaagacaaag gcaatgctaa cttcattttc tagttcatcg
     8761 taataaaatt ctaaattgtt ttcaccacga ataaaatcga agcgttcgcc attaattgtg
     8821 ccagtaaagg ccgcagcagc agtaaatgcc gcattaacct tatagcgttt acctacttcg
     8881 ccagggaatt tgacgaataa tttaagtgcg tcataagcga caccatcttc aacagtaaat
     8941 tggatgcgag gtacattatc aacaattcga gcatatgtga gatttacctt ttcaccaact
     9001 tgtaaataaa tttcattagg atcggcaaga gcagcagcag cgtcccctaa tttgattggc
     9061 tcacctggtt caacttctga gcttggtggc tcttcagagc ttaccggtgg ctcacttggc
     9121 tgttctgaag atactggtgg ctcttcagtc gtcacacttg ttacaggtgg ctgttccgaa
     9181 cttactggcg gtttttcact tgaaggtgtt ttaaaacaac ccgcaagtaa agagagacca
     9241 agtaataaca tgattaattt tgatgttctt tttttcatat tttctccttt catcaaactt
     9301 tattagtaat ctttttttcg cctcttaatt atgggtagtg ctcgttccta cagtggcgaa
     9361 aagatgtgct aactacttat atttttgaat taagtcattc acctccttta ctttttcaac
     9421 tggaagtttt ggcttccggt catatgttaa tagtccatta atttcttgtt caacatcagt
     9481 taattgcgta tagcaatatc cgacaagagc agtgctttct ttaattgcta aaataatgcg
     9541 tttatattca cttagtaaat gctctgcgtc accaaccgca gtatagcccc aacccttatc
     9601 ttcattcatt tgataactaa ttcccccaaa ttcagttaaa agaatcggtt caccgcgata
     9661 actataaccc tctgcatata tatcgcgatt cgctggtctt gacttcagta attcttcttt
     9721 tgtgctaatt gatttattaa aagcagcaat ggcgtcttta tcatcatgac gaccatgatt
     9781 ataattatga atcgcacaaa tgtcggtaat tatttgttcc catccgtcat tcccaacaac
     9841 gaagcgcgta ttatctaaac ttttaattaa ataatacaat tcaagtgcat gttgttgttg
     9901 ccgtttatcg tgaccaatat catcaacacc ccaactttca ttaagtggga cccacgcgac
     9961 gattgacgga tgattatagt cgcgagcaat aatttgtggc cattcatgtt tatcacgcca
    10021 cgctgatttt tcactaaagc gaatcgaact agccatttca ccccacacta agaaacctaa
    10081 tttatcagcg taataagcaa aataaggatc ttcacatttt tgatgcttac gtgccccgtt
    10141 aaagcccatt gctttcgcca tttttatatc atattctagc gcttctttac tcggtggcgt
    10201 cactaagctt tgtggccaat aaccttggtc gagcacaagt ttcatataat agggcttatt
    10261 atttaaatat gtaatcccct tatcagcgtg aaccttgcgc ataccgaagt aacttgaaat
    10321 caaatcatca ccataccgta atttaaacgg gaataaatgc gggttttctg gtgaccacgc
    10381 ttttgttata aaatcttgct cgtctaaaaa agtttgaaca gtaaattcat gtgttaattt
    10441 attaggtact ttagcatatt taattttgcc attttcatct tcaagaataa gttctctacc
    10501 aacttgacta aaacttaagg aaaaatgaac cttaccttca tcaaaaagtg gggtaatgcg
    10561 catactttta acatatgcct ctggaacgat ttctaaccac actgtttgcc aaattccggt
    10621 cgtgcggtta taccagatta cttctgattt ttctttccag tattgtttac cacgcggcat
    10681 acttaattct gtacttgggt cactaaccca cactactagt tcttcttcat taaaatttaa
    10741 aaaatcagtg acatcaaaac taaatggaac ttggccacca atatgatcac caatatgttt
    10801 tttatttaaa tatattttac attcataatc aaccgcacca aaatgtaaga taaagcgttt
    10861 accttcttct tttttaacac taaagaaacg ccgataccac acataatcat gaaaactagg
    10921 gtcattaatc ccgcttaatg gcgtttggaa cgcaaacggt acatttattt ttaagggaaa
    10981 ctttcccttt tcaacccaat tttcgtttaa cccaacatta tcatcatcaa aagcaaaatc
    11041 ccatattccg tttagattta gccactcttt gcgcacaaga gcgggacgcg gatattcttt
    11101 gcgatacata tagttaatgc tcccttctat ttaataccgg tgtgactgac accttcaata
    11161 atcttatctt gaacaaacat ataaacgatt aatactggaa taatcgaaat aatcgctgct
    11221 gccattactt gaccggggtc ataattactt tgactttgca ttgtcgctaa cccagtttgt
    11281 aatgtatacc aattttgatc aaagtgcact gcagcggtta aagtcggcca taagtagtca
    11341 ttccaacttc ccataaaagc aaatagtccc gcaaccatca ccgcggattt ccctaagggt
    11401 aaaataattt tacgatacac accaaaatgg cctaagcctt caatttgcgc actttcgact
    11461 aagtcatatg gtatattcat aaagaattgc cgcataataa agagcccaaa tgttccgcct
    11521 agtcctggca caataagcgc aaaaattgat ttccatgctg cttcactatc aagccagcca
    11581 aaacgaatga ccatcgtaaa tagcggcgtt aagttaatca ccgtcggaat cgccattgtc
    11641 gctagtaaaa aaccaaaaac aatattacga cccttccagc gcattacccc aaaagcatag
    11701 gctgccatgg aagcaataat taaatataaa agcgtatgaa ttatcgcaat aagcgcacta
    11761 ttgcgtaacc atttaaaaat aggatatacc gcgctattat caaataaatt tttataatta
    11821 ccaaaggtaa attcacgcgg aataatcgta attacattcg tgcgcatatc gtttgcgctt
    11881 ttaaacgaag taccaagcgc ccataaaagc ggtagtaccc ataagatgct cagcactaag
    11941 agaataataa atgcgacacg tttccggcgc ttcactccac ttttaattgc cattagtatt
    12001 tctccctata tttgttgtat gcagcttgcc caagactaac aactaagata aatgaaccaa
    12061 aaattagtga catcgccgct gctaaaccag gttgacgatt aacaccagtc attaacccgc
    12121 gaattaccat cattacaaca gttgtcgtgc ctggtgtacc agccgcgaca gtcccgcttg
    12181 gtgctggtcc accacccgtt aagatatcgg gctgaccata aatattaaaa ctagcgataa
    12241 ttgtcgtaaa cacaatgata aagatttgat ttttaatact aggcaaagca atactccaaa
    12301 tcacccgcgg ataactagcg ccatcaactt ccgcagcttc gtacatctgt ttatcgactt
    12361 cttttaagcc ggcgccaata atgactgtat ttgtcccaat tgtccaccaa attgtcgtta
    12421 ctacaatcgc aatccatgca taaggttgcg aattaagcca tgcaacttct ttaccaccga
    12481 acacgcgaat taacccattt acaaagccat attgcgtatc aaattgccat ttgaaaatga
    12541 tcccaaccgc ggaaattgaa agaatcgtcg gtaagaaaaa gattactcga aatactttgt
    12601 aaaagcgtgg ttggatatca attaagattg caataaagat cggaataata attaataagg
    12661 gaacagaaac acagacgaaa agtagcgtgt tctttagtga accccaaaac atctttgcgt
    12721 aatacgttac attcgttgta ccaattgtta agcctgagat cgctaatttg tagttatcaa
    12781 agccaataaa cttactagca cttggatcaa agacgttata gcggaaaaag gaaatataaa
    12841 caccaaagaa aagcggaaag ataaagaatg tcgcaaataa ggcgatataa ggcaatagga
    12901 agaaggccac tgttaagtag cgtttaattc cttctttcag ctttaatttt tgtttggcat
    12961 ccatgattta tatcaacttt cctttaacct tcaagttgcg caattaagac ggcttgttca
    13021 ccttcttccg ccgcagcgtt aagtaaagcc atcgcatcat atgtagcgtc actcatggcc
    13081 gttgttacac ggctaaagac tggtgcaaaa gcttcgtaat aatatggaga aggttcatta
    13141 agtgtataca tattaacatc accataagtt tgatgatatg ggagcgctaa atattcctcg
    13201 gtattacgcg caatattact tgcgggaata tgtccgccac tttctgccca taaatatgca
    13261 ttttcagtaa catatttaat aaacactaat gcagcttgtt gcttgattgt ttcatttcgc
    13321 ccttgtggta aaacaaaggt gtgactacgg cttgcaactt ttttgctatc cgcagttggt
    13381 gtatcaacaa acatatccgc taaggaagta acaccaaaat taattcctga atcaacaaga
    13441 ctattaagca tccagttccc gttaatgtgg aacattgctt tcccttgttg gaacatgagt
    13501 aaatcagcat caacattcac attaagtggt gaaatctttt caatatgaat taaatccgcc
    13561 actgctttca gtgctttttc cccagcagtg ttattaaagg ctggcattaa agtattttta
    13621 tcaatttctt tcccgccatt ttgatataaa gctgttgtaa aaatatattc acttggccat
    13681 gttgttgaaa gcggtaagcc ccagccacca gcattagttt gtggcatctt tttcgccgca
    13741 gcgattaatt ctgcgcgatt agtcgggact tcgcagccat gttgcgcaag taaatcttta
    13801 ttgtaataaa gcataatgct atgtacatca agcgggacac catattgctt attgtcccaa
    13861 atcatcgcat catacacatc ttcaatataa ttttcacgcg ttatatcaac tcccgtcgat
    13921 gctgttaaat tatcgagcgg gaataataag tttttattag cgaatgatgg tacacgataa
    13981 ctatgaatta aagcaatatc aaatgaacgt ttcattggaa ttgtcgcaac aagtgattca
    14041 tagaaatcta cttccggcat aaatgattca gtcaccgtaa tttgtccttg atattcttca
    14101 ttaaattcac ggacaaggcg acgcattgct gttccatctg cacccgtaat tgggttaccg
    14161 taagttaagg cgacattctc aaatgtcggt aatgatactt caacactacc ttcaccacct
    14221 ccaccgggtt ggctaggttt ttcacttgtt tgtccgcagc ccgttaatac gagagcaagt
    14281 agtaataatg gaatcgtttt ctttttcata atttttacct ttcctaaatg tatattgtta
    14341 gcgtggtgca atataaggtc tctcggcccc acgtgaaggt cctaagcctt caacacttgg
    14401 ccaatattcc ttgtcccaca ctaatttatc aattaataat gcacggcgat ttgtagtccc
    14461 gtaataagga tcttcaaatt tatcaaaacc gtgataaaca atataacttt caccagcgtc
    14521 atctttgaca attgagttat gcccaacccc agcaaagtca tcattaccac gcacaactag
    14581 cgtgcctaga ttttgccgat aagtcattaa gttcccagct ttgtcgtaat aaggtcctaa
    14641 gatactttca ctgcgacaca cacgcacttc ataagttgag tggtgacctt cgcaacatgt
    14701 ccccgttgaa aggaacatat agtaataacc gcgctcacga atgatataag caccttcgta
    14761 atttccaatg tcccatcgct tgttatcaac accagcgaca agtactttat tagcgtttgc
    14821 ttcagcgatt gtacctttga cgctaagccc atcagcagtt aattccacag cgtaaatgcc
    14881 gctaaaactg ccccaaaaca tgtaaacacg attatctgta tcaacaaata ctgcttggtc
    14941 aattgaatta taaacaccaa tttcattact atcaaagatt ttgccttggt cagtaaatgg
    15001 gcctaagggt gaaggccctg ttgctacacc aatcccagga ttaggatcgc cccatgctga
    15061 aagcgaataa tataagttat atgtattacc aattttaacg atatctggtg cccataaccc
    15121 cgcacctgaa gtttcactcc atgttggcat tgttgcgggt ttaaaggctt gatcacgata
    15181 ttcccaatca acaagattgg tgctacttaa gattggtgta taccgaacac caaagtaatc
    15241 accccataac gcattatctt gcgtaccata ggcataataa gtcccatcat ccgcgcgaat
    15301 cacagcaggg tcagctaaaa cgggttccca cactggattt tcataagttg tgggtacttc
    15361 ttccaaactt tggctagtta ctacatcttc actagtggga ggtagtgttt cactagaaac
    15421 tggaggagta catcccacta gcaaggtgca taaaataaga gtaaatccta tctttctttt
    15481 cattatggtt taattcctat cgctacgctt tttactgtaa ttactgtgac ttcgctagtg
    15541 ccaagtaaga gcgcacattt tccgagtgta aatgtttgtg atggtgtaaa ttccatcgtg
    15601 tactctgtta aggttgcact taaagtaata tcttcacggg ctaaaactgt ttcaccatta
    15661 ttattcgtta cttccatcgc aatcgtcgtt gccgctaatg cacttgcgct aactttcata
    15721 atgtaagtct tgccttccgt aaatgttttc ccatcttgat taatttgcac atgccaattt
    15781 gccgttcctg gactaatgac atcaatctta atttgctcat tcgcttgatc agcgatgaaa
    15841 tcaacttccg caccatgcgt catctcttgt gtccagccat agtaaccata tctaaagtca
    15901 ccattaatta cttcataatc ttcataatca cgaagatcaa ttgttgttgc ttcgtcagca
    15961 ttaatcatat cattatcata tttatcaagt gtgacttgat ttgcgccttg cgccccaagt
    16021 aaaaggctaa ctttcgctaa acaagtaaca gttgacttaa attcaagtaa gaatgtttga
    16081 tattccgtcg ttaacgaaat tagtttagtt gcgactcgtg aataactttg ggaaggatta
    16141 ctaatttcaa ttgtggcctt acgttcaata ctagctttag caattaaagt tacacgataa
    16201 atctttcctg cttgaatact cacattgctt tgataaaact gaatgtgcca gttatcatta
    16261 ctaccatttg ttatatcaat cgttatcgta ccgtcttcat tatcaataaa gtttgcggca
    16321 ccagtgccat tagtttgatt aacatctcgt acccaccctt ggagtccatt attaaagtca
    16381 ccattaatta agttatacgg gttatcacgc gcaatttgat aactaacaga gcgattaata
    16441 aagttaaaat taccttcggt gtccgtaacc cgataactaa caagcatccg tgtgacagcc
    16501 gcgattgaat caggaattgc accaattctt tcaatgcggt cactaatgtc gttaccttta
    16561 taatccacag cggtaactgg cggtaatgct tcaaattggt caccagtgaa tacagtaact
    16621 ggtccagcat tactaaacac tggacgttta ctttcttccg gtaaaacaat ttctctaacc
    16681 ttaatatcat cgacaactaa atcaagtgga tttgccttac ttgctgtacc atcaatatct
    16741 aaaccgcgtc ctaaataaag cactggttta actgacggat aatcagcatc tgccgtgtaa
    16801 atagctgaat aagtttggaa ttcactagtt agtaccattg ctggataacc aggaattaac
    16861 atagcaaaac cgttataatt ttcaaacccg gcagaaattg aacgcccttt atcactttta
    16921 gcgataaaag tcatttcgta agtacgccca gcactaaagg taatccccgg ttgttggtaa
    16981 agctgtaagg accacgcttc gctccctgta tcggtaaagc taaagacggc ctcttcgtta
    17041 atgacattaa atgtcccctg cccacctggt gtatcaaaac gccaataagt tttacccatc
    17101 gcaaagttac cgttaaacac attacagcct ttgacaactt gcaccatccg atactttgta
    17161 cttttagtgc caacactatt tgtcgcttcg tatttaattg tgtattgcga tacatagtaa
    17221 caatcaaaat agtcatcatc agcgacgaca acttctagtt caccatcaac actgtcgacg
    17281 gcgcgaaccc catcaagtaa tgtaaattcg tctccaatcg taattacaat atcatcagtg
    17341 ccgtaccacg taattgttcg ttcaccttct tctgaaccaa ctgtagatgt tgtacttact
    17401 tgcggttcgc ttgtctttcc gctttcacta ccatcacctt tacaagagcc aagtaacact
    17461 aatgaaagta gtgccaacac tagatagcga tgttttttca taatcaagtc tccttttcct
    17521 ttgatttatt taccatcaat tatttaatta aacataagga tacgaaacta tcatggtagt
    17581 ttctgaagtt taagttgtgg ggcactaatt tcgatctatt tttattgaat tattggtatg
    17641 attttgtttt ttagattatc gttaatctta cttacacctt tattttataa agcgtagcaa
    17701 aaatagtcaa ggacaaacta attaattcgt cattaattat tttcatcact taagtataat
    17761 tcaggtcctg gccgataatt attactaggc acattaccta atgtactcgg ccaattatca
    17821 gcattccata tgagtttatc aattaataac ttgcgggcat tacgcgtttc cactgttgca
    17881 tcaaatccgt gataaagaat ccaatatgtt ccagcatcat cacgcaagat tgaattatga
    17941 ccaacaccaa taaagaaatc attttgatgg acgacggaat gtccaacatt agcgtttttc
    18001 atactacgat tatatgaatc aaagtattcc cctaaaatac tttcacttcg cccaacgaca
    18061 acattataag tataatcacc agaacaacat gtaccagtcg ataaaaacat atagtagtaa
    18121 ttatcttttt taataatgta tacaccttcg aaggtcgata tatcaaagtt acgatttgtt
    18181 tcaaaaccag cgatatgcac cttattattc gctgcttgtt caacacttcc gtctttaaaa
    18241 ccaaggccat ccgctgttaa ttcaattgcg tatatacctc taaaactacc ccaaaacata
    18301 taaacacgtt catcttcatc gacaaacacc attgggtcaa tactattatt tacaccaatt
    18361 tcttgtgata aaaagaattt accgtgatca gtccagggtc ctgctgggtg cgtcgcagtc
    18421 gctatcccaa ttccggggtt aggatcatcc caccttgata atgaataata cattacatat
    18481 tgtccgttga tgtattggac atcaggtgcc cagatatttg caccaggtgt gccccaagtt
    18541 ggacgcgcta tggcattact tagtgcttcc cattccacta gattagcgct tttaataatg
    18601 cgtccaccag tggcaaatgt ataaaattgt ccatctgtgt gacggacaat actcggatca
    18661 ggaaaatcag cattataaat tggattttgg aaaaagactg ttgtaagttc acgcggtttt
    18721 tcacttgaag caatcgtttc actaagtgaa tcatcactta tttcacttac aacttcactt
    18781 ccagtaatcg gttcttccct gctagaagtt tggttacaac taacaagtag cgctacacaa
    18841 agtagcggca acaatatccg atagcgtttc atactcttcc cccttctatt ctactacact
    18901 atggtagtat ggtccaattt gctccgtcgt cgaaggcgta aaaccttcaa catatggcca
    18961 tccattttca tcaaataata atttatcttg taataaaact cgcgcacttt gtttgtcaac
    19021 atgataagcg tgatagtaaa tccacggttc gccgttaaca tcatcaatta ctgagttatg
    19081 accagtgccg acaaatgtat catttttctg taatactagt ttgcccgctg aacgattgcg
    19141 catatccgtg cctgtatcat cataataagg accatatggt gaaagggcgc gcccgactac
    19201 tactttgtaa gtagagctaa gtccttcaca acatgtaccc attgataaga ataaataata
    19261 gtagccatcc caataacgaa tataggctgc ttcgtaagtt tcagcgtccc agccgtgcac
    19321 cgtatctaag cccgcgactc ttattttact aagtgccgcc tgttcaacgc ttcccgcttt
    19381 aaacccaaga ccgtcacgcg taagctccac taaatagtta ccacgcattg aaccccacac
    19441 taaataaaca cggttagtgt taggatcaac aaaagcattt gcgtcaatac tattattcac
    19501 accaatttct tgtgataaaa ataatttacc gtgatcagtc cacggcccat ttggttttgg
    19561 cgcagtagcg taaccaatcc ccggattagg atcgccccat gttgaaagtg agtaatacat
    19621 tacatattgt ccgtttatat attgcacatc tggtgcccaa agtcctgctc cttttgtccc
    19681 ccaatctggt aaatgaccaa agacccaacc tttttgaccc caagttacgc catcgctcga
    19741 gcgtaaatat tggccacctg ttgcatataa aaaccacatg cgttgaattg ggtcatatac
    19801 tcctgaaggg tcagggaaat cataaaaata cacagagttc gtgtatgtac ttcttttcat
    19861 ttcaagttcc acgctaatac taggctcctc actacttgat aactctttac tacttggttc
    19921 aggttcactg ctttgcactt cgctttcact tgagggtact tcgcttgttg atagctcttc
    19981 actactttca tttgcactcg aaaacgtttc actactactt gaagcaattg ggagcgaatc
    20041 tttttcacta cttgtaggga aaagtgcaca actagaaatt accatcataa atactaagac
    20101 cattaaatgg cgaacatgtc gttttatttt cataagcgcc ccctattata gtttttcaaa
    20161 ggttatcgtt aatctaatta taaaacgaaa aaagtttttt gtaaacaaag cgtcgcacta
    20221 attgttaatc taatttattt tgcaccgaat atatgccata attattttat ggaaaagaat
    20281 aataaagagc gcgtaacatt aaaagatatt gcggaacgtg caggtgtaac tgttaatagt
    20341 gtttcacgtg cattacgtgg tcataaaagt atttctgaac cgacaaaaga aagagtaaga
    20401 gcgattgcta aagaattagg ctatatccct gatgcgcaag ctaggctgat gcgtggggca
    20461 aaattaagag ttattgctgt agtctacgat aatattgtta atccttacta tgcaattatg
    20521 aataatcatc ttaataatga attaacaaag gccaattacc ggccaatggt attttttgaa
    20581 agtagtcaag acggttcttt atcacttgaa gttgcccgtg aaatcatctc aatgcgcatt
    20641 gtcggtgtta ttacttattt agtgccaagt aaagaagtta ttgagctttt tgaagaccac
    20701 aatgtcccca ttgtactcct aggaggcact ggggatgcac tcgggattga tagcgttgcg
    20761 tcaaacgatt atcgtggtgg taaactcgca ggttataaat tatttgattt aggtggtaaa
    20821 aactttgcct acattggtgt gacccgcaag cttaatatta ataatgagcg acttaacggg
    20881 ttcttaaaag cacttaaaaa taatgatgtt atcgttcctg aagcaaacat tattcttaac
    20941 gaaaagcaac aaacaacccg tcaactaatg gacacgcttg atgtgcaaaa gaaaaagatt
    21001 gattccattt tttgtttcaa tgaccaaata gcctacgaag taattggcta cttaagtgaa
    21061 aaaggaataa aagtacctga agatattaat attatcggct ttgataattt acaaagcgca
    21121 attatttatc caattaaatt aacaacaatt gacgccgata aaccgcgtgc cgcccattta
    21181 gcgctcgtga gtttattctc taaaattaaa gatagtgaag atactgaagt aactggaaaa
    21241 atagttgatg ttacacttgt tgaaggtaac acaactaaaa tgcgaaaata agtccttttt
    21301 gccgcaaata attaataaaa atataaaaaa agataacgcg acgaagcgtt atctttcatt
    21361 ttaattaaaa attaatctta ttttaaaaga tcagcggcaa caccagcaac accaccaagt
    21421 ccagcaagga ctaaagcgac gacggcacca ccagtaagtt tataagcttc tagtgctttt
    21481 gctggttcat caacatttgg cataaccgca aagtttttaa gaagtgctag taatacaaca
    21541 ctcactaagc caagcgctaa aatgacaaag ccaagaagtt tatttttttc taaaccttta
    21601 acgactaaac ggccaacact aagtccaacc ccagcaagga gaagtaataa gactaagaag
    21661 tttaagaaac taaacttaaa aataataact tctactccaa ggagcgtttc agttcttcca
    21721 aaggctaaat caaggaaggt gtaacttact tctggttgag ttctagcaat cgcaggaacg
    21781 gcaaaccatc agatgagggc aactaaaaat aaaccagctg cgactacatt taataatttt
    21841 ttcttttgca taaaatataa agttaggcaa ctattaactt tccaaaatca caaaataata
    21901 aacggttgtt catttagtaa ataagcactt aaaaccgaat aattagcgta taatgggcat
    21961 aagttattaa gtacttctct ttggaaaagt gatattaaaa aatattttac aattagtaat
    22021 gaacaaagcc ccgttttgtt cacacttttc accttaaaat aaaggacttt atcttgcttt
    22081 taatttagtg ctaatattag taattcaaga ctaaatgtaa agattatgaa tgcttcgcat
    22141 aaccctttac catcactaag gcacaattat gataaaatta ttacgatgaa ttattcataa
    22201 ggaaagaaat tcatagttac tactcttaca tttctttagt gttgaattaa tagtagtaat
    22261 aatgaattta gaaaggacaa attaatatgt taacacgagt tattggatta gatatcggtg
    22321 ccgctactat gcgtgccgcg gttgtggacc gtgagggaac aatttattat agtaaggaag
    22381 ttgcaacacc agaacgcagt gaattacgct ttgtgcgaga aataaatgat ttattagaaa
    22441 atgttttatt tcatgcccct gaagcaatcg ccatcggtgt tggtgttcct ggtattgtta
    22501 gccccggcac tacattaatt catctcttac caaacttagg ttacgaaaat attgacttta
    22561 aacgaatact ccgtaaagaa ataaagcaac ctgtctattt acttaacgac gctaacgcag
    22621 cgtgttatgc tgaagcactt tttggtgccg gtgctgataa acgcatcgtc caatatatta
    22681 ctttatcgac cggtgttggt ggcggacttg ttattaacaa agaaatttat acaggagcac
    22741 gtggctttgc tttagaaatc ggcaatatga ttattgatac agaaacaccg cgccctaatg
    22801 acaaaatgaa tcgtaatagt tttgaaggga gttgttcggg taaaagttta tataaccaag
    22861 caaaacgctt tggcacgaat attcataccg tcgcaagtgt gtttaacgaa agccgtttct
    22921 ccccgattag aaatgtgtgg gttgataact tagggaaagg tatcgctaat atttattcac
    22981 tctatgatcc tgatgttgta attcttggtg gtgctgtgat gcaaagtgcg gataaattct
    23041 ttgatgatat tttaccagca gtcgaaaagt atacgctacc gcaacttcat ccgtatatta
    23101 atttaaaacg gtcacacttt ggtgaaacag gcgggatgat tggtgccgcg ctttatgctc
    23161 ttcacgaaca tgaaaaaata attaacaaag caaaaagtga cgaagtaaaa atagacactg
    23221 aagtaaaagc agaaactaaa taattattta taatcagtgc gattcgctaa taaaatacca
    23281 agtgttgtaa gcaaggctaa atacccaaca ttcgtcaatg ttgcccataa ccattcaagc
    23341 tttattggtg tagcaattcc agataaatat aaaaagggga atatataaaa taaaactttg
    23401 gctttaaaac cagtcccccc attaaaagaa aaga
//
LOCUS       DAOSLP010000015        21762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_2665_length_21762_cov_9.318423, whole genome shotgun sequence.
ACCESSION   DAOSLP010000015 DAOSLP010000000
VERSION     DAOSLP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 21762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21762
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2665_length_21762_cov_9.318423"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1407)
                     /locus_tag="PK460_02385"
     CDS             complement(172..1407)
                     /locus_tag="PK460_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE53975.1"
                     /translation="MGFKLFFKIVKANFVSFLAYVLILVVSGLMFGLSDSGAANEDFK
                     ILAPVYYANITNETVDDLPSDNTLIFDYLMDNNLISEESDDPFSQGFYTYTLNFVNVK
                     KNIKPSEVNDAIYAGVVRGALIIPKDLSTLTRENVLFIFHTNSQDLATITPTKAALNK
                     FINTYHDLKEVSATAGNNLSATELTNKTLIALEKETEIVYENPNRTKLSMLSHFFSFS
                     TYIMSAVIILLVSIVMYEIKRREILMRISITPYSIPRLIVGLIFGAITLSLLLVGLVY
                     VLAIISYPKIALSITGLYLTLNIMLFTLPLTALAMVIGLVISKAEIVSIISTVFALAQ
                     AFFSGAFLPTFLLPKSIINVGKVFPASHTVTINNLLAEGLNTNGPSILLNIGILLAYF
                     AVFLTIAIILSKKITKREG"
     gene            complement(1409..2575)
                     /locus_tag="PK460_02390"
     CDS             complement(1409..2575)
                     /locus_tag="PK460_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE53976.1"
                     /translation="MKSLFILNLKKLLKNKEIVFWALLFPIILSTIFHIVFDGIRNRD
                     NFETLKVNYVSTNFTAEDKAFLVEEMLTNKAEYIKNEGTDKAQTVKIFALSEKTKAEA
                     EKEFKKDKTIYFYKEDGAFKIKASSQNISTAILQTFIDDYQNFIKVTTLLVAESNGTL
                     SFQDAMNKHVASYAYTYDFDSGDKKIDYVNNYHYTVLAMAIIYGAFLATEQAKIFQPN
                     SFAFAKRVTVSGASRAKLLVSALLSSLVVQTAIIALYIAILVFIFKVTFANIGLALLA
                     ALIGVILVNINGFFIGIVFNKLSQNAVIAIIILAGTIGGFLSGMMISSMKFFVGTKMP
                     LLSYININALISDSFVSLDFGNMTRYWNNMLAMTVIGVILFGVTLIKFNRESNK"
     gene            complement(2577..3518)
                     /locus_tag="PK460_02395"
     CDS             complement(2577..3518)
                     /locus_tag="PK460_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE53977.1"
                     /translation="MINMIKVKNLHKTYKKGVKAVNGLNFKVKAGEIFGLLGPNGSGK
                     TTTINAILDLVDYETGEIILFDNKYKTKVEAKKHIGLITQEVGVFNELTVRENIDYFC
                     GLYINDEKLRAQYVEEAMDFVQIKDFAKFKPKKLSGGLLRRLHIACGIAHKPTLIFFD
                     EPTVGIDPQSRNHILEQIKVLNKNGATIVYTSHYMEEIEMLCDYILIMDKGQVIAEGN
                     YDALIDQIDLAEYIEIEYKGEMPLKVVTEINELPGILEVIHTKEKLVIKTSEDDKAFI
                     KIIHILENNNVDYSRANANKPSLNDVFLELTGKELRD"
     gene            complement(3655..4413)
                     /locus_tag="PK460_02400"
     CDS             complement(3655..4413)
                     /locus_tag="PK460_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOE53978.1"
                     /translation="MRRNANPIFRNMNNDMNQAMTSSVPVDVTVSTASWSGIALKTLL
                     LVLIMIAAGLSTWFLPFPVVVGFLIFGGIASFFFVFFAMRSVKTAPVLSVLYAITQGI
                     MYGALTFIVETYFPGVGVMALVATFGIVGVMAFLHSIKAVRATPGLIRFVFAALLMVM
                     ISSLVLFIISRVAQDLYLSYVDNYPLMVIVSAFLVVLGAIMLILDFNNAETIVQSGAP
                     KIYEWQVSLGFLVTLVWIYLQVIRFLLVLARNRE"
     gene            complement(4452..5168)
                     /locus_tag="PK460_02405"
     CDS             complement(4452..5168)
                     /locus_tag="PK460_02405"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE53979.1"
                     /translation="MGRAFQVRAASMASTAAKKSALNMRASKEIFMVAKSGGTDPNSN
                     LALRAALDKYRAMGATKDVMERAIKKAEGDDAFAYIPGRYEVMGPGGSQLVIDTLTDN
                     VNRAISDIKAVLKKTDSILQTVAYNFTETGIFLFAGNNLEEITETLILNDVDVWDINE
                     EDDVIEVLVNPTSYGKTRDTLYSLGIKEFNISEIQMIPNDKVTLNAEDTEVFKRIIDM
                     LEDVQDVQEVYHNVNVPTEE"
     gene            complement(5204..5929)
                     /gene="truA"
                     /locus_tag="PK460_02410"
     CDS             complement(5204..5929)
                     /gene="truA"
                     /locus_tag="PK460_02410"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOE53980.1"
                     /translation="MRYVAIVKYLGTNYFGWQRQPFEISVQQVIEDKLSTIFNTEIVI
                     YASGRTDARVHAYGQVFHFDAPPFPVSKLKYALNKMLPGDIEIVKLQKAKDDFHARYA
                     AKSKTYEYLIYLGAKDPFLFNRVLFYPFALDEKKLRKDAKLFVGTHNFQNFTSKKEDE
                     ANFVRNISAVKIQKSGKMLKIVFIGEGFMRGQIRMMVGALLAQNAGKEDANFIKDNLN
                     SKERKIISHKVIGDGLYLRKVKY"
     gene            complement(5929..6813)
                     /locus_tag="PK460_02415"
     CDS             complement(5929..6813)
                     /locus_tag="PK460_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOE53981.1"
                     /translation="MKNISLGRYMPADSFMHKADPRIKFFGLVVFMVLIFLKFANPAM
                     DFLMYGIYAVVVFILMKVTKLKIFSLFKQLKSLWFMMIFLLIINIFTFRNSEGNILFM
                     IGNFAIKDAALYQTLYIFIRLVLMIALTSVLTASTRPLDLTYAIEWYMSPLKVIRFPV
                     HEIAMTLSIALRFIPTLLDETGRIMRAQESRGVDFKGGKVKEKLRGVVALIVPLFVSA
                     LQRSDELANAMIARGYDPQAKRTRYRILRFTWRDLIVLLLAVSFLTLVILLRVYHIDL
                     ANMLYVNIQNLLSLWGLK"
     gene            complement(6810..7769)
                     /locus_tag="PK460_02420"
     CDS             complement(6810..7769)
                     /locus_tag="PK460_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOE53982.1"
                     /translation="MAIKFHHIYFSYAPKSPFQYDALHDINLEIKQNSFTAVIGHTGS
                     GKSTLVQHINALLVPTSGAVKVEDFLILPCKKKKYLRQLNRKLKSKKVSAEQKQRLAF
                     LKSIVESGAAYKVKELRKKVGVVFQFPEYQLFEETVIKDVAFGPKNFGVSNEEALALA
                     KNALTLVGIDESFYSRSPFELSGGEKRRVAIAGILALKPEILVLDEPTAGLDPLSAHE
                     MMEVFANIHRAGTTIVLVTHDMDLVLRYASDVVVMKDGKIVEETTPQKLFADVKEEYS
                     LEVPAFYAFMQALVQRGCPLDITKINTVSDLAKMVKNLKEQAK"
     gene            complement(7760..8575)
                     /locus_tag="PK460_02425"
     CDS             complement(7760..8575)
                     /locus_tag="PK460_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOE53983.1"
                     /translation="MNKYALEIMNLNYSYDKNVEVLNGVTFNVKAGTYVSVIGHNGSG
                     KSTLAKLIAGILDVGKGSVIKVFGKPLSSYDDERPDIGIVFQNPDNQFIGATVRDDIA
                     FGLENKRVAHAEMDDIINEYAAKVKMSEFLDKEPSNLSGGQKQRVAIAGVLAMKPRIL
                     LLDEATAMLDPRGKREIKEIVTELRKEVPDLTIISITHDLEETLHSDEIIVMNEGKIV
                     LQGTPEDIYKNHETLTSIRLDLPFIFRIKLELEKQGIKVKSKDLESLAEELWP"
     gene            complement(8568..9047)
                     /locus_tag="PK460_02430"
     CDS             complement(8568..9047)
                     /locus_tag="PK460_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOE53984.1"
                     /translation="MKRKDYINWDEYFMGIAILSSKRSKDPSTQVGACIADDENKVVS
                     LGYNGMPRGIDDDVIPWEKGEDINNKYFYVCHAELNAILNTRSGASLKGCRLYVTLFP
                     CNECAKACIQVGIKEIVYLDDKYHEQVNMRASRHMLKLAGVKLTPFKGKVLDPKFNE"
     gene            complement(9114..10745)
                     /locus_tag="PK460_02435"
     CDS             complement(9114..10745)
                     /locus_tag="PK460_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOE53985.1"
                     /translation="MNNKWWHERIFYQIYPRSFKDTNGDGVGDIPGIISKLPYLKSLN
                     VGAVWISPLYASPNFDNGYDISDYKAIHPEYGTMEDMERLLAQAKKLDIKIIMDLVIN
                     HTSFQHDWFLKSKDPKSEYHNYYFWRKGKISKKGKKLPPNNWQSMFTGSAWTYEPSND
                     MWYLHLFTREQPDLNYENPKVLEEVKSILRFWLDKGVAGFRCDVINLISKTSLKDGKK
                     RVYKTGKEHYLNAPGCHLILKELNDEILEPYAAYTVGETMDITIDAAKDYLDGELTQV
                     FAFDQVQVDYFLLPTFKRKYKPHRMVNALKKWQLKIPWNTVFYENHDLPRSVSRFGSK
                     KYYKESVTALLASVLFLRGTPYIYQGQEIGMTNVDFGGIENIEDVSSRNVYNLLRKFK
                     VPRKLAWRFVNNFTRDHGRSPVQWNDKKHAGFTTGKPWLMVNPNYTEINVEAQEIDQD
                     SVLHHYRKMATLRQKTKAFKNGDLEFKKTHRDILAFTRTDDLNEYLVVINLASKARTH
                     KLNLDGEVLYSNYQEFSDKAKKLRPFEVQIIKRLD"
     gene            complement(10832..10960)
                     /locus_tag="PK460_02440"
     CDS             complement(10832..10960)
                     /locus_tag="PK460_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE53986.1"
                     /translation="MFEELLFTYDPRTKKEEQREDDDTLEMAQALALYEKHLEDNN"
     gene            complement(11160..11972)
                     /locus_tag="PK460_02445"
     CDS             complement(11160..11972)
                     /locus_tag="PK460_02445"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOE53987.1"
                     /translation="MLKTKRLVTALTLSAFLIAISVVIQRFLVIPFGMPSLYRLSLGN
                     IPIIMASLYLGPVFGAIVGAASDLIGATLFPVGTLIIWPVISSTLYGVVPWLILRLVM
                     YLDRKIKVPLFYVFLAIIFIGLETYIFVKPSIRHPFNSTLDPIMFTTTFRIVFTLVLL
                     LIFSGLIITFNVLVKKYKEGAYEKYTGAPTSLAFTLMLMTFFVDILYSSWWKMFQFKV
                     DFFVSVFFHTLIMFILLPFQVVLLLILSNVYAKSRVAELLALPPKEHIDTDD"
     gene            complement(11965..13185)
                     /locus_tag="PK460_02450"
     CDS             complement(11965..13185)
                     /locus_tag="PK460_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOE53988.1"
                     /translation="MTKEQIKEYLTNTPTRYDKRAYDSFLKEHGIPKLPPAIHITGTN
                     GKGSTAYYLALSLQNSGYNVGLYTSPSFKNVNELACINGEEISDEYISECIKKYDEDF
                     KKYELSLFEIMTFISFNYFADNNLDYVVIEVGMGGLLDATNVFTPLISIITNIGLDHL
                     DTLGPAITDVAEHKAGVIKKGVPVLLGDAVRRAEKVVLDTALKLNAPVYRTHNNALVT
                     RHDLERINFTYNGEHYTLYSGALYEVNNAVAALDAIEILKSLGVSIKKSAIKAAFKTK
                     PFEGRFNVIQKEPAIIVDGLHNPHAIRALLSDIKVYGKNLKVVFAAFNDKDYFQEIDL
                     LILAGATITLTTFDHPRAVRDFGSTQLPYIGEHKVAIKEAVKSLKKNDMLLIVGSLYF
                     ARLVIDEFKRGEYA"
     gene            complement(13178..13660)
                     /gene="smpB"
                     /locus_tag="PK460_02455"
     CDS             complement(13178..13660)
                     /gene="smpB"
                     /locus_tag="PK460_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOE53989.1"
                     /translation="MAKQKKTNNVITENRRASHDYFLSDFLEVGIVLKGTEIKSLRHS
                     GASLKDTYIVFRNGEAFIEGLYIAPYAFGNIFNHEPRRTRKLLMHKREIARYSANVQI
                     ASMTCVATKMYFVRGRVKLTIALGKGKKLHDKRDAIREREDKLAMAKALKQERSSRDD
                     "
     gene            complement(13678..14583)
                     /locus_tag="PK460_02460"
     CDS             complement(13678..14583)
                     /locus_tag="PK460_02460"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE53990.1"
                     /translation="MENYSHKYKISPELTLYGAYWGVKKPRANVLVITGMQEHAMRYT
                     EFAKFLNEAEFSVYSIDYFGQGENVTQGGHTLGVLPNHGFEKFVTALGTFVENLAKDK
                     LPLYVLGHSMGSFLAQRFVQRYPLLSKKVVIVGSNGPSALFGMGKAIAALTVNNRNRD
                     KESKLLASMAIGAYSKAVKNAHTPADWLSYNEDNVNTYLAHPLDGKPSSKGFYLELLR
                     GTSNLYKKKHYVDVPRDLPIFIIAGAEDPVGANGKGIIKLGKFYAKLGFTNLEVKIYE
                     GMRHEILNETDRRDVYADVLSFLMR"
     gene            complement(14701..15702)
                     /gene="galE"
                     /locus_tag="PK460_02465"
     CDS             complement(14701..15702)
                     /gene="galE"
                     /locus_tag="PK460_02465"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOE53991.1"
                     /translation="MKILVTGGTGFIGSHTVVALLEEGHEVVIADNLVNSQRNVVTKI
                     KRITGVKPFFYKVDVADKKSMRKVFSEHKFDAVIHFAGLKAVGESVQKPLLYYRNNLD
                     STLTLLELMKEFDVKNILFSSSATVYGTPENVPLKEEDPIGGTTNPYGTTKLIIEQIL
                     MDFVHANEGFKAVSLRYFNPIGAHPSKLLGESPKDIPNNLMPYISRVANGKLSHLNVF
                     GDDYETPDGTGIRDYVHVLDLANGHVLALKAFSFNHAYNVYNLGTGKGTSVLELVHLY
                     EKANKLKIPYVIGPRRDGDIAASYADITKAKNELGFVPHYSVKDACRHAYLFEKKQK"
     gene            complement(15714..16268)
                     /locus_tag="PK460_02470"
     CDS             complement(15714..16268)
                     /locus_tag="PK460_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOE53992.1"
                     /translation="MAKNIMVVTGSVRKTGNSIMLANAFVKGARNNGNAIQKVEASHR
                     NFSPFRDKENAWRKGKPSLVEDDFNVLAPYLHVSDVLVLASPVYFGGFSGAIKVFIDN
                     LYAYVVEHRKRNLRFTHAILLAVARDDNPDTFVGITHEFKELCRQLGIEKMDIITVPG
                     VAERGDIVGHEALDRARDLGKTIS"
     gene            complement(16339..17268)
                     /locus_tag="PK460_02475"
     CDS             complement(16339..17268)
                     /locus_tag="PK460_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE53993.1"
                     /translation="MNILMILTPKSEVIYLSTKMRVRDAMEVLKKARFNSVPLLDESG
                     HYVGTVTEGDILWHLDQNGYEDSLDMRLKNVKRIRDYAPVSVDEEIDELIHKALGQNF
                     IPILDDRKYFIGIVTRKNLISTYIKKVDDNKEEVVHKNEVLDNLYKRRSIRRFKEGIT
                     IPQEIIDEIVKVSLVTPTAMNRRPHEIMLVDDPQVIKTLSELHQRGGQFEKAPYLILI
                     LNDDEKEPNAYLANSNCSALLISLLLAVESFDNLGGFWVSTRYPEYNRKMLNAVGVPE
                     RFSLYGMVAFGVKGEHKPANEPLNPEKIHRNKW"
     gene            17388..18212
                     /gene="nagB"
                     /locus_tag="PK460_02480"
     CDS             17388..18212
                     /gene="nagB"
                     /locus_tag="PK460_02480"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOE53994.1"
                     /translation="MTIFIRDNYNEMSKAAAKIIAKQIKEKPDSVIGLATGFTPIGTY
                     RELIRMHKEEGLSFKGVTAFTLYEFLGEGFDIIKPYGLDKSSARYMHEEFFKHVDIDK
                     KNIHYFDGFTDDPRRTCQEYEEKIKAAGGIDLQLLGIGRDGHWGYNEPGSSLGSRSHV
                     VALTKQTIDDNYEDFYRKVGITKDEMPHFALTMGVGTILDARKLILVASGAKKAEIVQ
                     KALEGPITAQITSTAIQLYAGEATIILDADAASMLSNIEHIKHTEAMSKKYGFRAE"
     gene            complement(18241..19668)
                     /locus_tag="PK460_02485"
     CDS             complement(18241..19668)
                     /locus_tag="PK460_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOE53995.1"
                     /translation="MATILIVGLTFFFLEQSIITDVTPGYYFDPLTLSNQMMVTGIIV
                     TLVSIFSFFVIRKIRLSVNGIILCIVLTIVFTMALVNLLIMPKEQLLSIRFLDGTYHD
                     INFVLPTRMRLTAIMTLVVNFSFIFFVVLVLPNHVSFLKLVLFFTIAIVLIAFFAIGY
                     SLATEWEKYVAIVKEGPGSTYQVPASFFNNRNPYASFLLNAQVMLFFLYYKNLDKKRR
                     FFYILLQIPLVLAINFTYSKTNLLLANILLIIAFLFHLVRLLGRKKFIRFGIEVFLAF
                     LLVIGFIVFRYSPKLETTNISLFLHRTLPDEMFERVPGSFDSRKKLWYYAVSLMVANP
                     IIFVFGHGPHLSRYFYHLRMQIEIEGVMTPGFGDYHNGLVEVMHTFGASGLIIYALVF
                     LFVTILLIRRARTNRSLTFFVLLSMGVFILRSQMESLAVLLFKSEGIMASLTFVLPAL
                     YLLRKSHECGFSKELKVKKTRPEEA"
     gene            complement(19826..20863)
                     /locus_tag="PK460_02490"
     CDS             complement(19826..20863)
                     /locus_tag="PK460_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOE53996.1"
                     /translation="MKKKAITLVVLLAMLVGCSRNESSSAPSVSSEGEVSTSEVNSET
                     TSEESSVASSEELPSSEELSSEEPSSEEPPSSSENPTNYPDTPAEIEVPQSVFEYYAS
                     VDFNKTGNNLKLELYNKIKGHVSTSYNELNATMAITDRNWDLSPDPNDSDPYMVLIYA
                     SYNDTNPKKYSTRNNDVWDKEHIWAKSHGNFDNTPPAGSDMHHLRASDKRNNGSRGNL
                     DFGKANSVKSTINDYDGKPSGKTGTQTGKSGTVYEPLPQYRGDVARAMFYMATRYHND
                     TQTQLTLVDNTTSSSTGVGKFGILSTLLEWHLQDPVDEFEFRRNGLVQGYQNNRNPYI
                     DFPSLAHDVFG"
     gene            complement(20898..>21762)
                     /locus_tag="PK460_02495"
     CDS             complement(20898..>21762)
                     /locus_tag="PK460_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOE53997.1"
                     /translation="SEDSSSIASSEESSVSVSSEPGVPYNVTFVTNCETMLDAIYTDV
                     IYDMPLIFREGYTLAGWYLEATFINIVSFPYYVTSDVTLYAKWTEGETAHFTFELHPN
                     NNGYIVTSYGGNAINVVVPTTYNDKPIVALGEKLFYQNGSIKSVVLPATLTYIGMQAF
                     KDAVSLESIVIPSNVTELATDAFAGTISLAQVTLSSKLKIIGNNAFENSAFAEVTIPS
                     LVTEISSRAFAGNTNLLSVNLISTTPPLRFANSFEGTVSTMKYYVPSSAYNSYINSPY
                     WQAYSAQIVVK"
BASE COUNT         6979 a         4221 c         3481 g         7081 t
ORIGIN      
        1 tgtgttgttt gacgtagcat atttgtattt ctcctttacc aattttcgtc ttaccgggac
       61 gacaaaaggc agataatgcc gtgattaata ataatatatt ataaatatat tgtcaactta
      121 attcgttatt ttatttatca atataattga taagccgaca taaagatgaa attaaccttc
      181 gcgtttcgta attttcttgc taagaatgat ggcaattgta aggaatactg caaagtaggc
      241 tagtaaaata ccgatattta ataagatgct tggtccgttt gtatttaatc cttcggcaag
      301 caagttatta attgtcactg tatgactagc gggaaagact ttcccaacat taataatcga
      361 cttaggtaag aggaatgttg gtaaaaaagc tccagagaaa aaggcttgcg ctaaagcaaa
      421 gactgtacta atgatggaga cgatttcagc tttactaatt acaaggccaa tgaccatcgc
      481 tagtgccgta agtggtaaag taaatagcat aatattaagt gttaagtata atccagttat
      541 gcttaaagca atttttggat aagaaataat tgctaagaca taaactaggc ctactaacag
      601 aagcgaaagg gttattgctc caaagataag gcccacgatt aagcgcggga tgctgtaggg
      661 cgttatcgaa atacgcatta atatttcacg gcgtttaatt tcatacatta caatactaac
      721 aagcaagata ataactgctg acataatgta agttgaaaat gaaaagaaat ggcttaacat
      781 cgacagtttt gtgcgatttg gattttcata aactatctcc gtttcttttt caagcgcaat
      841 taatgttttg tttgttaact cagtagcgga taagttattc ccggctgtag cactaacttc
      901 ttttaagtcg tggtaagtat taataaactt attaagggcg gcttttgttg gggttattgt
      961 cgctaaatct tgcgaattcg tatgaaaaat aaataataca ttttcccgcg ttaaagttga
     1021 taaatccttc ggaataatga gtgcgccacg cacaactccg gcataaatag cgtcatttac
     1081 ttcacttggc ttaatatttt tctttacatt cacaaagttt aaagtgtatg tataaaagcc
     1141 ttgggaaaac ggatcatcac tttcttcact aattaaatta ttgtccatta aataatcgaa
     1201 aattaaagtg ttatcacttg gtaagtcatc gactgtttca tttgtaatat ttgcgtagta
     1261 gactggcgct aaaattttaa agtcttcatt agcggcgcca ctatcgctta acccaaacat
     1321 aagaccacta acaactaaaa ttaaaacata agcgagaaat gaaacgaagt ttgctttaac
     1381 aattttgaaa aataatttaa accccatctt atttattgct ctcccgatta aatttaataa
     1441 gcgttacacc aaagagaatg acaccgataa cggtcatcgc tagcatatta ttccagtaac
     1501 gagtcatatt accaaaatca agactaacaa atgaatcact aattaatgcg ttgatattta
     1561 tataagacaa aagcggcatt ttagtgccaa caaaaaactt cattgatgat atcatcatcc
     1621 ctgagaggaa cccgccgata gtacccgcta aaataatgat agcaatcact gcattttgac
     1681 taagtttatt aaagacaatc ccaatgaaaa agccatttat attcactaaa ataacaccaa
     1741 ttaatgcagc taaaagcgct aaaccaatat tagcaaatgt cactttaaaa atgaaaacta
     1801 atattgcaat gtaaagcgca ataattgctg tttgcacaac gagtgaacta agtaaggcac
     1861 tcactaataa tttagcgcga ctagcgccac taactgtaac gcgtttagca aaagcaaaac
     1921 tatttggttg aaagatttta gcttgttctg tcgcaagaaa tgccccataa attatcgcca
     1981 tcgcaagtac tgtatagtga tagttattaa cataatctat ttttttatca ccagaatcaa
     2041 agtcatatgt ataagcatag cttgcaacat gtttattcat ggcgtcttga aatgataatg
     2101 taccattact ttctgcgact aagagtgtcg taaccttaat aaagttttga tagtcatcaa
     2161 taaatgtttg taatatcgcg gtacttatat tttgtgaaga tgctttaatt ttaaatgccc
     2221 catcttcctt ataaaaataa atggtcttat cttttttaaa ttctttttcg gcttcagcct
     2281 tagttttttc gctaagtgca aagatcttta ctgtttgtgc tttatctgtg ccttcgtttt
     2341 taatgtattc agctttgtta gtgagcattt cttcaactaa aaacgctttg tcctcggcag
     2401 taaagtttgt ggaaacataa tttactttaa gtgtttcaaa gttatcacga ttgcgaatac
     2461 catcaaacac aatatgaaaa atagtactta atataattgg aaataataac gcccaaaaaa
     2521 caatttcttt atttttaagt aatttcttta aatttaaaat aaataaactt ttcatcttag
     2581 tcccgtaact ctttaccagt taactccaaa aatacatcat ttaaacttgg tttattcgca
     2641 ttagcccgcg aataatcaac attgttattt tctaaaatat gaataatttt aataaatgct
     2701 ttatcgtctt cactcgtctt aattactaac ttctccttag tgtggatcac ttcaagaata
     2761 cctggtaatt catttatttc tgtcacaact tttagcggca tttcgccttt atattcaatt
     2821 tcaatgtatt ccgctaaatc gatttgatca attaaggcat cataatttcc ttcggcaata
     2881 acttggcctt tgtccataat taaaatatag tcacatagca tttcaatttc ttccatgtaa
     2941 tgcgaagtat aaacaattgt cgccccgttt ttatttaata ctttaatttg ttctaagatg
     3001 tgattacgac tctgtgggtc aatcccaacg gttggttcat caaagaaaat aagcgttggt
     3061 ttatgcgcaa tgccgcacgc tatatgtaat ctccggagta atccgcctga aagtttcttt
     3121 ggtttaaact ttgcaaaatc tttaatttgc acaaagtcca tcgcttcttc aacatattgt
     3181 gcacggagtt tttcatcatt tatataaagt ccgcagaaat aatcaatatt ttctctaact
     3241 gttaattcat taaagacacc aacttcttgg gtaataagtc cgatatgctt tttggcttca
     3301 actttagtct tatatttatt atcaaataaa ataatttcgc ctgtttcata atccactaaa
     3361 tcaagaatag cgttgatcgt tgtcgtttta ccgctaccat taggacctaa taagccaaaa
     3421 atttctccag ctttaacttt aaaattaaga ccatttacag ccttaacacc ttttttatat
     3481 gttttgtgta agttttttac ttttatcata ttaatcacct ataagcatta taacttcatt
     3541 tatgtgattt ttatcagtat aagcaattcg taaaggtttc tttacaaagt aaagcgcgac
     3601 aataaagaaa aaacgaccac atgtagtggc cgtctttttg ctaacatata ttccttattc
     3661 tcgattccga gcgagaacaa gaagaaagcg aatgacttgt aaataaatcc acacaagggt
     3721 aactaagaac cctaaactta cttgccattc ataaattttt ggcgctccgc tttggacgat
     3781 tgtttcagca ttattaaagt caagaatgag cataatcgcc ccaagcacga ctaagaatgc
     3841 actgacaatg accatcagcg ggtaattatc aacataactt aaatataaat cttgggcaac
     3901 acggctaata atgaaaagta ctaaactact aatcattacc attaataagg ctgcaaaaac
     3961 aaaacgaatt aagcctggtg tagcgcgtac agctttaata ctatgtaaga aagccatcac
     4021 accaacaatg ccaaaagtcg ccacaagtgc cattacacca acaccaggga agtaggtttc
     4081 aacaataaat gtcagtgcgc catacattat cccctgagtt attgcatata acacacttaa
     4141 aacaggagca gttttcacac ttcgcattgc gaagaacacg aagaagaatg aagcaatacc
     4201 accaaagatt aaaaaaccga cgacgacagg gaatggtaaa aaccatgtac taagtcccgc
     4261 agcaatcata atgagtacga gtaataatgt tttaagggca atgccgctcc atgatgctgt
     4321 gctaactgtc acatcaactg gcacactact tgtcattgct tgattcatat cattattcat
     4381 attacggaat attggatttg catttctacg catataaagt tcctccttat atttttgcca
     4441 ctaatattat atcactcttc agtgggcaca ttaacattat gatacacttc ttggacatct
     4501 tggacatctt ctagcatatc aataatgcgt ttaaatactt cagtgtcttc tgcatttagt
     4561 gttactttat cgttaggaat catttgaatt tcactaatgt taaattcctt aatccctaaa
     4621 gaatataatg tatcgcgtgt tttaccgtat gaagtcggat taacaagaac ttcaatcaca
     4681 tcatcttctt cattaatatc ccatacatca acatcattaa gaattaatgt ctctgttatt
     4741 tcttcaagat tattgcctgc gaataaaaag atacctgttt cggtaaagtt ataagcaact
     4801 gtttgtaaga tcgagtccgt ctttttaagc acagctttaa tatcactaat cgcgcgatta
     4861 acattgtcag ttaaagtatc aattacaagt tgactgccac ctgggcccat cacttcgtaa
     4921 cgccctggaa tataagcaaa agcgtcatct ccttccgcct ttttaatcgc ccgttccatg
     4981 acatcttttg tcgcacccat tgctcgatat ttatcaagcg ctgcgcgtaa ggctaagttg
     5041 gaattaggat cagtgcctcc gctttttgcc accataaaaa tttcttttga ggcccgcata
     5101 ttaagtgcac tctttttcgc tgcggtactt gccatacttg ccgctctaac ttgaaatgct
     5161 cttcccataa ctttgctcct ttaactacta ttttattata agcctaatac tttactttgc
     5221 gtaaataaag cccatcacca attactttat gactaatgat ttttcgttct tttgaattta
     5281 agttgtcttt aataaagttt gcatcttctt tacccgcatt ttgcgcgagt aatgcgccaa
     5341 ccatcatcct aatttggcca cgcataaatc cttcaccaat aaacacaatc tttagcattt
     5401 tgccgctctt ttgtattttc actgcactaa tattacggac aaaattagcc tcatcttctt
     5461 tcttacttgt gaagttttgg aagttatgtg taccgacaaa taatttagcg tctttccgta
     5521 actttttctc atctaatgca aaaggataaa aaagcactct attaaataaa aacggatctt
     5581 ttgcacctaa gtaaattaga tattcatatg tcttactttt agcggcatac cgcgcatgaa
     5641 agtcatcttt tgctttttgt aatttaacaa tttcaatatc accaggtagc attttattta
     5701 aggcatactt taatttacta acaggaaaag gcggagcgtc aaaatgaaac acttgcccgt
     5761 aagcatgaac tctagcgtcc gttctacctg aagcataaat tacaatttcc gtattaaaaa
     5821 ttgttgataa cttatcttct attacttgtt ggacactaat ttcaaagggt tgcctttgcc
     5881 acccaaagta atttgtgcct aagtacttta ctattgcgac gtagcgcatt acttaagccc
     5941 ccaaagagat agtaaatttt ggatattaac atagagcata tttgctaaat caatatgata
     6001 gacacgtagt aaaataacaa gcgttaaaaa actaaccgct agtaataaaa caattaaatc
     6061 acgccaagta aaccgtaaaa tccgataacg cgttctttta gcctgtgggt catacccgcg
     6121 cgcaatcatc gcattagcga gctcatcgct gcgttggagg gcactaacaa aaagcggaac
     6181 aataagtgcg acaacaccgc gtaatttttc ttttaccttc ccgcctttaa aatcaacacc
     6241 acgactttct tgcgctcgca taattcgtcc cgtttcatca agtaaagtcg gaataaaccg
     6301 gagtgcaatt gacagtgtca tcgctatttc atgaactgga aaacgaataa ctttaagcgg
     6361 actcatatac cactcaatcg cataagttaa gtctagcggt ctcgtacttg cggttaaaac
     6421 cgatgttaaa gcaatcatta acacaaggcg aatgaaaatg tatagtgttt gataaagcgc
     6481 tgcatcttta atcgcaaagt taccaatcat gaataagata ttaccttcac tatttctaaa
     6541 tgtaaatata ttaattatta gtaagaaaat catcataaac cacaagcttt ttagttgttt
     6601 aaagagtgaa aaaatcttta attttgttac tttcattaaa ataaaaacaa caacagcgta
     6661 aatcccgtac atcaaaaagt ccatggcagg attagcaaac tttaggaaga ttaaaaccat
     6721 aaatacgact aaaccaaaaa atttaatccg tggatcagct ttatgcataa atgaatcagc
     6781 aggcatataa cgccctaagg agatattttt cactttgctt gctccttaag gtttttcacc
     6841 atttttgcta aatcacttac agtatttatt ttagtaatat caagtggaca gccgcgctgc
     6901 actaacgctt gcataaaagc atagaatgct ggtacttcta aactatattc ttctttcaca
     6961 tcagcgaaaa gtttttgtgg tgttgtttct tcgacaatct tcccgtcttt catcaccacc
     7021 acatcactag cgtaacgcag cactaaatcc atatcatgtg tgactaacac gattgttgtc
     7081 cccgctctgt gaatattagc gaaaacttcc atcatttcat gggcgcttaa aggatctaga
     7141 cccgcagttg gttcatcaag cactaaaatt tcaggcttaa gcgctaaaat accagcaatt
     7201 gccacccggc gtttttcacc accggaaagt tcaaatgggg aacgactata aaaggattca
     7261 tcaatcccga ctaatgttaa agcgtttttc gctaacgcta gtgcttcttc attactcacc
     7321 ccaaaatttt tcgggccaaa agcaacatct ttaatgacgg tctcttcaaa tagttgatat
     7381 tcaggaaact gaaagacaac gccaactttt tttcgtaatt ctttaacttt ataagcagcg
     7441 ccactttcaa caatactttt taaaaacgct agtctttgct tttgttcggc acttactttt
     7501 ttacttttaa gcttacggtt taattgtcgt aagtactttt tctttttaca aggcaaaata
     7561 agaaaatctt caactttaac ggcaccagaa gttgggacta aaagtgcatt aatatgttga
     7621 acaagcgttg attttccgct accagtgtga ccaataaccg ctgtaaaact attttgctta
     7681 atttctaaat taatatcatg caatgcatca tattgaaaag gagactttgg cgcataacta
     7741 aaataaatat ggtgaaactt tatggccata attcctccgc aagactttct aaatctttac
     7801 tcttaacttt aattccttgt ttttcaagtt caagtttaat ccggaaaata aagggtaaat
     7861 caaggcgaat actcgttaat gtttcgtgat tcttataaat gtcttccggt gtaccttgca
     7921 gcacaatttt gccttcattc atcacaataa tttcatcact atgtaatgtt tcttctaagt
     7981 catgcgtaat actgatgatt gttaagtctg gtacttcttt tcttaactct gtcacaattt
     8041 ctttaatctc gcgtttccca cgtggatcaa gcatcgcggt cgcttcatct agtagtaaaa
     8101 tgcgtggttt catcgctaac acaccagcaa tcgccacgcg ttgcttttgt ccaccactta
     8161 agttgcttgg ttctttatct aagaactcac tcatttttac tttagcggcg tattcattaa
     8221 ttatgtcatc catctcggca tgggctactc ttttattttc gagaccaaaa gcaatatcat
     8281 cacgcaccgt tgcaccaata aattggttat cggggttttg aaaaacaatc ccgatatctg
     8341 gtcgttcatc atcataacta gataagggct taccaaatac tttaatgaca ctccctttgc
     8401 cgacatctaa aataccggca ataagtttcg ctaatgttga tttcccactg ccgttatgac
     8461 cgataacact aacataagtg cccgctttaa cattaaaagt gacgccgttt aaaacttcga
     8521 catttttgtc gtaagagtaa tttaagttca ttatttctaa ggcatattta ttcattaaat
     8581 ttggggtcta acactttacc tttaaatggt gttaatttca ctcctgctaa tttaagcata
     8641 tggcgactag cgcgcatatt cacttgttca tgatacttgt catcaagata cactatttct
     8701 ttaatcccga cttgaatgca agctttagcg cattcgttac atgggaaaag tgttacatac
     8761 aagcgacatc ctttaagtga ggctccactt cttgtattta aaatggcatt aagttcagcg
     8821 tgacatacgt aaaaatattt attattgata tcttcacctt tttcccacgg aattacatca
     8881 tcatcaattc cgcgaggcat cccgttataa cctaaactga cgactttatt ttcatcgtca
     8941 gcaatacatg caccaacttg ggtacttggg tctttacttc ttttcgaaga aagaatcgca
     9001 attcccataa aatattcatc ccaattaata taatctttac gtttcatgat tagcctccct
     9061 taacagcgct aattatagca taaatgctat aggttaaggg ctagttaaga gtcttaatct
     9121 aagcgtttta taatttgcac ttcaaatggg cgcaactttt tagccttatc actaaactct
     9181 tgatagttgg aataaagtac ttcaccatca agatttaact tatgagtgcg tgctttactt
     9241 gctaaattaa tgacgactaa atattcgttt aaatcatctg tgcgcgtaaa cgctaaaatg
     9301 tcacgatgtg ttttcttaaa ttctaggtcg ccatttttaa aagcttttgt tttttgccgt
     9361 aaagtcgcca tttttcgata atgatgtaat actgagtctt gatctatttc ctgagcttca
     9421 acattaattt ctgtatagtt tggattcacc attaaccacg gtttaccagt agtaaaacca
     9481 gcgtgctttt tatcattcca ctgtaccgga ctgcggccat gatcgcgcgt aaagttattc
     9541 acaaagcgcc acgcaagttt acgcggtact ttaaatttac ggagtaagtt ataaacatta
     9601 cgcgaactta catcttcaat attttcaatg ccgccaaaat caacatttgt cattccaatt
     9661 tcttgtcctt gataaatata cggtgttccg cgtaagaata agactgacgc taaaagtgcc
     9721 gtaacacttt ccttataata ctttttacta ccaaagcgac tcacactacg tggcaagtca
     9781 tgattttcat aaaagactgt attccaaggt attttaagtt gccatttctt tagggcgtta
     9841 accatccgat gcggtttata tttccgttta aatgttggta ataaaaagta atcgacttgc
     9901 acttgatcaa aggcaaacac ttgcgttaat tcaccatcta aataatcttt agcagcgtca
     9961 atagtaatat ccattgtttc gccaaccgtg taagccgcgt aaggttctag tatttcatca
    10021 ttaagttctt ttaagattaa gtgacaccca ggtgcattta agtaatgctc ttttcctgtt
    10081 ttatacacgc gcttcttccc gtctttaagt gaagttttac taattaaatt aataacatca
    10141 caccggaaac ctgcgacacc tttatcaagc caaaagcgta aaatactttt aacttcttct
    10201 agtacttttg ggttttcata atttaaatca ggttgttctc gtgtaaaaag atgtaagtac
    10261 cacatgtcat tacttggttc ataagtccag gctgatccag taaacattga ttgccaatta
    10321 ttcggcggta attttttacc ttttttacta attttacctt tacgccaaaa gtaatagtta
    10381 tgatattcac ttttgggatc cttgctcttt aaaaaccaat catgctggaa ggaagtatga
    10441 ttaataacta agtccataat aattttaatg tcaagtttct tagcttgcgc aagtaatcgc
    10501 tccatgtctt ccatcgtgcc atattctgga tggattgctt tataatcact aatgtcgtac
    10561 ccattatcaa aatttgggga agcgtaaagc ggagaaatcc atactgcccc aacatttagt
    10621 gattttaaat atggtaattt agaaataatc ccaggaatat caccgacacc atcaccattt
    10681 gtgtctttaa aactccgtgg ataaatttga tagaaaatac gttcatgcca ccatttatta
    10741 ttcataattt tgtacctctc ttttagtgta tcaaaaaagc aataaaaaaa cgccctaagg
    10801 cgtaatttat taaaagtcac aataactttt attagttatt atcttctaaa tgcttttcat
    10861 aaagagcaag cgcttgtgcc atttccagcg tgtcatcatc ttcgcgttgt tcttcttttt
    10921 ttgttcgcgg atcgtacgta aataaaagtt cttcaaacat aagcatttac tcctttttta
    10981 actacgacta ttgtaagata atagccccta aaattcaaag gtatttaaga cactaataat
    11041 taatactata agtattaatt ttgacatatt gtgtctaatg cgtttaggag ttttttagta
    11101 gtttttaaaa aaggcgttaa ttcacgccta acttaaggtt taaaacatta taaaaaggct
    11161 taatcgtctg tatctatatg ttcttttggg ggtagcgcta aaagttctgc tactctgctt
    11221 ttggcgtata cattacttaa aattaataaa agcacaactt ggaatggtaa taaaataaac
    11281 atgattaaag tgtggaaaaa gacactgacg aaaaaatcaa ctttaaattg gaacatcttc
    11341 caccatgacg aataaagaat atcgacaaag aaagtcatca gcatcagtgt aaacgctagt
    11401 gaagttggtg caccagtata tttttcataa gcaccttctt tatatttttt aacaagcaca
    11461 ttaaaagtaa taatgagtcc gctaaagata aggagtaaaa caagcgtaaa aacaattcta
    11521 aaagttgtcg taaacataat cgggtcaagc gtgctattaa acgggtgtct aattgaaggc
    11581 ttaacaaaga tatatgtttc taagccaatg aaaataatag ctaaaaatac atagaaaagc
    11641 ggtactttga tttttctatc taaatacatc actaggcgta aaataagcca cggcacaaca
    11701 ccataaagtg ttgaactaat cactggccaa ataataaggg ttccgaccgg gaaaagtgtc
    11761 gccccaatta aatcacttgc cgcgcccaca atcgcaccaa atacggggcc aagataaagt
    11821 gaagccataa taatcggaat attaccaagt gataagcggt aaagtgaagg cattccaaaa
    11881 ggaattacta aaaagcgttg aataacgaca ctgatagcaa ttaaaaaagc acttagtgtt
    11941 aatgctgtca cgagtcgttt cgttttaagc atattcacct cttttaaatt catcaataac
    12001 aaggcgcgca aaataaagcg aaccaacaat taaaagcatg tcatttttct ttaatgattt
    12061 cactgcttct ttaattgcca ctttgtgctc accaatatac ggaagttgtg ttgatccaaa
    12121 atctctgaca gcccgcggat gatcaaaagt tgttaaagta attgtggccc ctgctaaaat
    12181 taataaatca atttcttgaa aataatcttt atcattaaaa gcagcaaaca ctactttgag
    12241 atttttacca tatactttaa tatcacttaa taaagcacga attgcatgtg ggttatgtaa
    12301 accatcaaca ataattgctg gttctttttg aataacatta aaacgacctt caaacggttt
    12361 agttttaaat gcagctttaa tcgcactttt tttaatacta acacctaaac tttttaatat
    12421 ctcaatggcg tcaagtgccg ctactgcatt atttacttca taaagtgctc cgctataaag
    12481 cgtgtaatgt tcaccattgt atgtaaaatt aatccgctct aaatcatgtc ttgtaacaag
    12541 tgcattatta tgcgtgcgat acactggcgc gttaagtttt aaagcagtgt caaggacaac
    12601 cttttcagcg cgtctaacag catcaccaag taagaccggc acaccttttt ttataacgcc
    12661 cgccttatgt tcagcaacat ccgtaattgc tggtccaagt gtatcaagat ggtcaagtcc
    12721 aatattagta atgatgctta taagcggggt aaaaacattc gtagcgtcaa gtaaaccgcc
    12781 cattcccact tcaataacga cataatcaag attattatcc gcaaaataat taaagctaat
    12841 aaatgtcatt atttcaaaaa gtgataattc atatttttta aagtcttcgt catacttttt
    12901 aatacattca ctaatatatt catcactaat ttcttcacca ttaatgcacg ctaattcgtt
    12961 aacattttta aaagatgggg atgtatataa gccgacatta tacccactat tttgcaaact
    13021 taaggcaagg taataggcag tacttccctt cccgttagtc cctgttatat gaattgcagg
    13081 cggtaatttt gggattccgt gttcctttaa aaatgagtca tacgctcttt tgtcgtaacg
    13141 agtcggggta ttagttaaat actctttaat ttgctcttta gtcatcacgc gaacttcttt
    13201 cttgctttaa agcctttgcc atcgctaatt tatcttcgcg ctctctaatt gcatcacgct
    13261 tgtcatgaag ttttttgcct ttaccaagcg caattgtaag cttgacacga ccacgaacaa
    13321 aatacatttt tgtggcgacg catgtcattg acgctatttg cacatttgcg ctatagcgtg
    13381 ctatttcgcg tttatgcatt aaaagtttac gtgtcctgcg tggttcgtga ttaaaaatat
    13441 taccaaaagc atatggagca atatatagcc cttcaatgaa agcttctccg ttacggaaaa
    13501 caatataagt atctttaagc gaggcaccac tgtggcgaag cgacttaatt tctgtgcctt
    13561 ttaaaacaat tcccacctct aaaaagtcac taagaaaata atcgtgactt gcacggcggt
    13621 tttctgttat aacattattc gtcttttttt gcttcgccac tatagtgtat ctttctttta
    13681 tctcattaag aaacttaaga catcagcgta aacatcacgc cgatctgttt catttaatat
    13741 ttcgtggcgc attccttcgt aaatcttcac ttctaaatta gtaaaaccaa gttttgcata
    13801 aaatttacct agtttaataa tacctttccc gttagcgcct actgggtctt cagcaccagc
    13861 aataataaag attggtaagt cacgcgggac atcaacataa tgctttttct tatataaatt
    13921 actcgtgccg cgtaataatt ctaaataaaa accttttgat gacggtttcc catcaagcgg
    13981 atgcgcgaga taagtattga cattgtcttc gttataacta agccaatcag cgggagtatg
    14041 cgcattttta acagcttttg agtaagcacc aattgccata ctagcaagta acttactttc
    14101 tttatcacgg ttacgattat taacggtgag agcggcaata gctttcccca ttccaaaaag
    14161 tgcagaagga ccgtttgatc caacaattac cactttctta gaaagaagcg gataacgttg
    14221 cacgaagcgt tgcgctaaga aactacccat tgaatgccct aaaacataaa gtggtaattt
    14281 atcttttgct aggttttcta caaaagtacc aagcgccgtc acaaattttt caaagccgtg
    14341 attaggtaaa acacctaaag tatgaccacc ttgcgtgaca ttttctcctt gtccaaaata
    14401 gtcgatacta tatacactaa attccgcttc atttaagaat tttgcaaatt cagtgtagcg
    14461 cattgcatgt tcttgcatcc ctgtgatgac taagacattc gcccgcggtt ttttaacacc
    14521 ccaataagca ccataaagtg taagttcggg ggagatttta tatttgtggc tgtaattttc
    14581 catattacgt cctccttctt ttaattttac catttattta catattgctt acataagtaa
    14641 aatgaaaata tttatggtta accattactc cttaattttt tgatataaat ataaaaatta
    14701 ttacttttgt tttttctcaa atagataagc gtggcgacaa gcatctttta ctgaataatg
    14761 cggaacaaag cctaattcat ttttagcttt tgtaatatca gcgtagcttg cggcaatatc
    14821 accatcacga cgtggaccaa tgacatacgg aattttaagt ttatttgctt tttcataaag
    14881 atgcactaat tcgaggacgc tagtgccttt gcctgtccct aagttataaa cattataagc
    14941 atgattgaaa ctaaaggctt taagcgctaa aacatgacca ttagctaaat caaggacatg
    15001 gacataatct ctaattcctg tcccatcagg cgtctcataa tcatcaccaa acacatttaa
    15061 gtgacttaat ttcccgttag cgacacggct aatgtaaggc attaagttat tgggaatatc
    15121 ctttggtgat tcaccaagta attttgaagg atgagcacca attggattaa aatagcgtaa
    15181 actaactgct ttaaaacctt cattagcgtg cacaaaatcc attaagattt gctcaataat
    15241 taactttgta gtgccatagg gattggtagt accgccgatt gggtcttctt ctttaagcgg
    15301 gacgttttca ggggtgccat ataccgttgc actactggaa aaaagaatgt ttttcacatc
    15361 aaattctttc atcagttcca gcagtgttaa tgttgaatct aagttgtttc tgtaataaag
    15421 gagcggtttt tgcacacttt caccaaccgc tttaagccca gcaaaatgaa tcacagcgtc
    15481 aaatttatgt tcgctgaaaa cctttcgcat gcttttttta tcagcaacat cgactttata
    15541 aaaaaacggc tttactcctg taatccgttt aattttagtg acgacatttc tttgtgagtt
    15601 cactaaatta tcagcaataa cgacttcatg accttcttct agtagtgcca caacggtatg
    15661 tgaaccgatg aagccagtgc ctccagtaac taatattttc ataaaactat ttcctaacta
    15721 attgtttttc ctaaatcccg cgcacggtca agtgcttcat gcccaacaat atcaccacgt
    15781 tcagctacac caggtacagt aataatatcc attttttcaa tacctaactg ccgacataat
    15841 tctttaaatt catgggtaat accaacaaat gtatcgggat tatcgtcacg cgctaccgca
    15901 aggagtattg catgcgtaaa gcgtaagttg cgtttacgat gttcgacaac ataagcataa
    15961 agattatcaa tgaatacttt gattgcgccg ctaaagccac caaagtaaac aggtgaagca
    16021 agcactaaaa catcactgac atgtaaatat ggggcaagga cattaaaatc gtcttcgact
    16081 aaacttggtt tacctttccg ccatgcattt tctttatcgc gaaatggtga aaaattacga
    16141 tgcgaagctt caaccttttg aatcgcatta ccattatttc gcgcgccttt aacaaaagca
    16201 tttgcaagca taattgagtt gcctgttttc cggacgctgc ctgtaactac cataatgttt
    16261 tttgccatat tgcctccatc aataattata tcataaaatt gttatattgt gcttaaaatc
    16321 gtgcctttta ttaagttttt accacttgtt acgatgaatt ttttctgggt taagtggttc
    16381 atttgccggt ttatgttcac ccttgactcc aaaggcaacc atcccgtaga gactaaagcg
    16441 ttctggtaca ccaacagcgt ttagcatctt cctgttatat tctggataac gggtactaac
    16501 ccagaaccca cctaaattat caaaactttc aacagcgagt aataagctaa taagtaaagc
    16561 actgcagtta ctatttgcaa gataagcatt tggttctttt tcatcatcat ttaatattaa
    16621 tattaaatat ggagcttttt caaattggcc gcctctttga tgtaattcgc taagtgtttt
    16681 gataacttgt ggatcatcga ctaacattat ttcatgtggg cgtcgattca ttgccgttgg
    16741 ggttactaaa ctaactttaa caatttcatc gattatttct tgtggaatag ttatgccttc
    16801 tttaaagcgt ctaatacttc ttcttttata taaattgtcg agcacttcat ttttatgaac
    16861 aacttcttct ttattatcat caaccttttt aatataagtg ctaattaagt tcttccgtgt
    16921 cacaatacca ataaagtact ttcgatcatc aagaattgga ataaagtttt gaccgagtgc
    16981 tttatgaatt aattcatcaa tctcttcgtc cactgataca ggagcgtaat ccctaatgcg
    17041 tttaacgttt tttaagcgca tatcaagtga atcttcataa ccattttgat caagatgcca
    17101 taagatgtca ccttcagtga cagtgccaac ataatgacca ctttcatcaa gtaaaggtac
    17161 actattaaat cgtgctttct ttaacacttc catcgcatct cttacacgca ttttagttga
    17221 taaatatatg acttcactct tcggggttag tatcattaaa atgttcatgc tccacctctt
    17281 tctatactat aattatataa taaaaccgct ttcactgtgg taaaaaaagg aactttgttg
    17341 tattatatgg tgtaactata tgttagacta tttatgagga gaatattatg actattttta
    17401 tacgtgacaa ttataacgag atgtcaaaag cagcagcaaa aataattgct aagcaaatta
    17461 aagaaaaacc tgattcagtt attggccttg cgacaggctt tacaccaatt ggcacttatc
    17521 gtgaactaat tcggatgcat aaagaagaag gtttaagttt taaaggagta acagctttta
    17581 ccctttatga attcttaggt gaaggttttg atattattaa gccttacgga ttagataaat
    17641 caagcgctcg ttacatgcat gaagaatttt tcaaacatgt cgacatcgat aagaaaaata
    17701 tccattattt tgacggattt accgatgatc cacgtcgcac ctgtcaagaa tatgaagaaa
    17761 aaattaaagc agcaggcgga attgacttac aactcttagg tatcggccgt gatggccact
    17821 ggggttataa cgaaccaggc tcaagtttag gtagtcgtag ccatgttgtt gctttaacta
    17881 aacaaacaat tgatgataac tatgaagact tttatcggaa agtaggaatt actaaagatg
    17941 aaatgccgca ctttgcctta acaatgggcg ttggcacaat tttagatgca agaaaattaa
    18001 tccttgtcgc tagcggagct aaaaaagcag aaattgtaca aaaagcactt gaaggaccaa
    18061 tcaccgccca aattacttct actgctattc agttatatgc tggcgaagca acaattattt
    18121 tagacgctga tgccgcaagt atgttatcaa atattgaaca cattaaacat accgaagcaa
    18181 tgagtaaaaa atacggcttc cgtgccgaat aatacttatt aatcatattt taagaaatga
    18241 ctaggcctct tctggcctag tttttttaac ttttaattct ttactaaagc cacattcatg
    18301 cgactttctt aataaataaa gcgcaggtaa tacgaatgtt agtgaagcca taataccttc
    18361 gcttttaaaa aggagaactg ctaaagattc catttgactc cgtaaaataa agacgcccat
    18421 actaagcaag acaaagaaag ttaaactgcg atttgtcctt gctcttctaa ttagtaatat
    18481 cgttacaaag agaaaaacaa gggcatatat tattaatccg cttgcgccaa aagtatgcat
    18541 tacttcgact aagccattat gataatcacc aaagcccggt gtcattactc cttctatttc
    18601 aatttgcatt cgtaagtgat agaaatagcg cgaaagatgt ggaccgtgac caaagacaaa
    18661 tattatcgga ttagcgacca taagactaac tgcgtaatac caaagtttct tccgtgaatc
    18721 aaaagagcca ggcacccttt caaacatttc atcagggagt gtccgatgta aaaataaaga
    18781 aatattcgtc gtttctaatt ttggcgaata acgaaagaca ataaatccaa ttacgagtaa
    18841 aaatgctaaa aacacttcaa taccaaagcg aataaacttc ttgcgaccta ataacctaac
    18901 cagatgaaat aaaaaagcaa taataagcag tatattagct aataataaat ttgttttaga
    18961 ataagtgaag ttaatagcta aaacaagcgg aatttgcagt aaaatataaa agaaacgtcg
    19021 ttttttatca agatttttat aatagagaaa aaagagcatt acctgcgcat tcagcaaaaa
    19081 cgacgcataa ggatttctgt tattaaaaaa actagcgggc acttggtaag tgctccctgg
    19141 accctctttt acaattgcaa catatttttc ccattcagta gcaagactgt acccaatagc
    19201 aaaaaaagcg attaagacaa ttgctatagt aaagaaaagc actaatttta aaaatgacac
    19261 atgatttggt aacacaagca cgacaaagaa aataaaacta aaattcacaa caagcgtcat
    19321 aatcgctgtt aaacgcatcc ttgttggtaa cacaaagttt atatcgtgat atgttccatc
    19381 taaaaagcga attgaaagca attgttcttt tggcattatt agcaagttaa caagtgccat
    19441 agtaaatact atagtaagta caatacacag gataataccg tttacactaa gacgaatctt
    19501 acggataacg aaaaaagaaa aaatactaac gagtgttaca atgattcctg ttaccatcat
    19561 ttgattagaa agtgttagcg gatcaaagta atatccagga gtaacatccg taattattga
    19621 ctgttctaaa aagaagaaag ttaagcccac aattagaatt gtggccaaaa agtatataaa
    19681 cagatttgct ctttccctga ctttactcat tttttaatta tacaactatt tgttcctaaa
    19741 tgttaatgca gataattgtg cggttaaaat gacacacatt ttatgtgtgt attttatttg
    19801 tgtttttttg ctataatttt ctttattaac cgaatacatc atgcgctaaa gatgggaagt
    19861 caatatacgg attacggttg ttttgataac cttgcactaa tccgtttctc ctaaattcaa
    19921 attcatcaac tgggtcttgt aagtgccatt ctaaaagagt agataaaatg ccaaatttac
    19981 ctactccagt tgaactactt gttgtattat cgacaagtgt taattgcgtt tgcgtatcat
    20041 tatggtaacg tgttgccata taaaacatcg cacgcgcaac atcaccgcga tattgtggta
    20101 gtggttcata aacggttcca cttttcccgg tttgcgttcc cgttttacca gaaggcttac
    20161 catcataatc attaatcgta gatttaacac tatttgcctt accaaagtct aaattaccac
    20221 ggctaccatt attgcgctta tcactagcgc gtaagtgatg catgtcactt cccgctggtg
    20281 gggtattatc aaagttaccg tgagacttcg cccaaatatg ttctttatcc catacatcat
    20341 tattacgggt agaatatttt ttaggattag tatcattata actagcgtaa attagcacca
    20401 tatatggatc actatcatta gggtccggtg ataagtccca gttacgatca gtaatagcca
    20461 ttgttgcgtt taattcgtta taacttgtac ttacatgccc tttaattttg ttatatagtt
    20521 ctagttttaa attgttgcct gttttattaa agtctacact tgcgtaatat tcaaacacac
    20581 tttgggggac ttcaatttcc gctggtgtat caggataatt agtcgggttt tcactgctac
    20641 taggcggttc ttcactcgaa ggttcttcgc ttgaaagttc ctcactactt ggcagctctt
    20701 cgctactcgc aacacttgat tcttcacttg tcgtttcact attaacttca ctagtggaca
    20761 cttctccttc actgcttaca gaaggtgcgc tacttgattc attacgcgaa cagcccacta
    20821 acattgctaa tagtactaca agtgtaattg ctttcttttt catacttttt tacctattta
    20881 tttttagacc tttctattta tttaaccaca atttgtgcgg aatatgcttg ccaataaggg
    20941 ctatttatat atgagttata tgctgaggaa ggcacataat atttcatcgt cgaaacagtg
    21001 ccttcaaatg aattagcgaa acgtaatggt ggtgtggttg atattaaatt aacacttagt
    21061 aaattagtgt taccagcaaa agcacgtgaa ctaatttcag taactaaact aggaatagtc
    21121 acttctgcaa aagcgctatt ttcaaaagcg ttattcccaa taatttttaa tttgctcgaa
    21181 agtgttactt gcgctaggct tattgttccg gcaaaagcat ccgttgctaa ctcagtaaca
    21241 ttgcttggaa taacaatact ttctaaacta actgcatctt taaaagcttg catcccaata
    21301 taagttaatg tggcaggtaa aacgacagac ttaatactac cattttgata gaaaagcttt
    21361 tcacctagtg ccacgatagg tttatcatta taagttgtgg gcacaacaac attaatcgca
    21421 ttaccaccgt aagatgtaac aatataacca ttattattag gatgtaattc aaaggtaaag
    21481 tgtgctgttt caccttcggt ccatttcgcg tacaatgtca catcactagt cacataataa
    21541 ggaaaactga cgatattaat aaatgttgct tctaagtacc atcctgcaag agtgtagcct
    21601 tctctaaaaa taagaggcat atcataaatt acatcggtgt agattgcatc aagcattgtt
    21661 tcacaatttg taacaaatgt aacattatag ggcactcctg gttcgctgct tacagaaaca
    21721 gaagattctt cactacttgc gatgcttgaa gagtcttcgc ta
//
LOCUS       DAOSLP010000016        21231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_2739_length_21231_cov_11.988525, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000016 DAOSLP010000000
VERSION     DAOSLP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 21231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21231
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2739_length_21231_cov_11.988525"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /gene="rplC"
                     /locus_tag="PK460_02500"
     CDS             complement(<1..558)
                     /gene="rplC"
                     /locus_tag="PK460_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOE53998.1"
                     /translation="MKAIIGRKMGMTQVFAEDGTIYPVTVVEVLPNVVTQVKTVEKDG
                     YAAIQVGYQDKKPSRVNKPLKGIYEKAGVTPKYGLYELKGEEMLSYEVGSEIKVDIFK
                     KGDIVDVTGTSKGKGYTGVIKRYGFKLGNAAHGSGYHRGIGSQASVGRIQRVIPGTKM
                     PGHHGAQRTTVLNLLVVDVLPEKNAL"
     gene            complement(571..882)
                     /gene="rpsJ"
                     /locus_tag="PK460_02505"
     CDS             complement(571..882)
                     /gene="rpsJ"
                     /locus_tag="PK460_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOE53999.1"
                     /translation="MAGKQKIRVRLQSYDHALLDEAVAKIVEQAVESGAKVVGPIPLP
                     TQREVYTVLRAVHKYKDAREQFEIRTHKRLIDIVDPNKRTVEFLRKFELPSGVDIDIK
                     L"
     gene            complement(1210..1872)
                     /locus_tag="PK460_02510"
     CDS             complement(1210..1872)
                     /locus_tag="PK460_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOE54000.1"
                     /translation="MEKINYYNISIAEVNNFKDQGLRPKLALHACCAPCSSHTLEFLT
                     PFFAVTIVYQNSNIYPKAEFKKRRDEVERFVREFNIKNNQDVKVVFLPYDHEAFMKEL
                     RVFASEKEGGKRCHLCYEKRIVEALKYGSENGFDYATTTLTISRQKNSQVINAIAKKI
                     APRYPNIKYFYSDFKKKAGLLRVREMKKEYNLYQQEYCGCEYSYRDTVLARNNKKDTE
                     NR"
     gene            complement(1859..2893)
                     /gene="queA"
                     /locus_tag="PK460_02515"
     CDS             complement(1859..2893)
                     /gene="queA"
                     /locus_tag="PK460_02515"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOE54001.1"
                     /translation="MDVNLFDFDLPKELIAQYPSLLRQNARLLAFNKDKNEIKDEHFY
                     DITKYFKHGDVLVINNTKVIPARLRGIKVPTGAKIEVVLLKALKDDEYEVLIGNAKAV
                     KVGTVVSFGDGELLMECREKKGEGIHIVKFKYEGVFLEVLERVGTTPLPPYIEDKSEK
                     YARYQTVYAKAPGSAAAPTAGFHFTPEILDELKTKGVIVVEITLHIGLGTFKPVSVSD
                     TKDHKMHYETYEISEEAAATINKAIEEKRRIISVGTTATRTLEANFNKYQKIVARSEA
                     TNLFITPGYKFKVISALITNFHLPKSTLIMLVSAFAGRENTLAAYCHAIKERYRFFSF
                     GDVMYIYGED"
     gene            complement(2893..4026)
                     /gene="tgt"
                     /locus_tag="PK460_02520"
     CDS             complement(2893..4026)
                     /gene="tgt"
                     /locus_tag="PK460_02520"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOE54002.1"
                     /translation="MAKKPPVYLEIIHECTQSGARYGFLHTPHGKVEVPMFMPVGTLA
                     NVKTITPEEIVALGSGVILANTYHLSLRPGTDILKKAGGIHSFMNHHGPILTDSGGFQ
                     VFSLADNRKISEEGVTFKSHLDGRELFFSPEEAIRMQEEIGADIIMSFDECIHYPADY
                     EYVKASTERTLRWAKRGKAAHKREDQALFGIVQGGEYPDLRKYSAMETVKIGFPGYAI
                     GGTSIGEPKDVFIKMVESSVPYLPKDKPRYLMGIGSIDMILQGVARGVDMFDCVLPTR
                     IARHGSLYTRKGRLNIRRAEFKDDFRPIEEECDCYTCKNYTRAYLRHLYVSEEALAQR
                     LLSIHNLRFLIKLMEDVRVAIKENRFLDFKSDVLKKFGDKRGF"
     gene            complement(4019..4525)
                     /locus_tag="PK460_02525"
     CDS             complement(4019..4525)
                     /locus_tag="PK460_02525"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOE54003.1"
                     /translation="MKKKKIIRQITDNALIAAIYYVLTIILGGFAFNDVQFRISEILL
                     FLIFFRKDFVIGVTLGCLLANLHSLLQPWDVIFGTLATLLSGVLIAFSKRLYWGVIWP
                     TIINAVVVGLMLHIILDLPLFATMGLVAIGEFVVLLLGHAVFSLLAKQPAFLNLIMAE
                     QRKEEKDG"
     gene            complement(4583..6010)
                     /gene="gatB"
                     /locus_tag="PK460_02530"
     CDS             complement(4583..6010)
                     /gene="gatB"
                     /locus_tag="PK460_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOE54004.1"
                     /translation="MKYEVIIGLEIHIETKTNTKMFSSASSLFDAPANSNVSVVDMAF
                     PGTLPTVNKKAVQNAIMLGHALNMEIDDELHFDRKNYFYSDLPKGYQITQQRRPIGSN
                     GYLDIYVNDTKKRIRIERIHMEEDTAKQIHLGEVTLIDYNRAGNPLVELVTEPDLRSA
                     DEAMAFVEAVREIVTFLEISDGKMEEGSLRCDVNISLRPVGVSKFGNKVEIKNMNSIS
                     NIGKAIETEIKRQSELLDKGLEVESETRRYDDALKDTITMRKKDESVDYRYIPEGNIL
                     PISLSKEFIDATIREMKELPSVKRERYFEEFKLNAHEVNILLQDKDLTSYFDEAARDV
                     KNYKTLINWVIGEFSSYLNKEGKNAKSVALSPQNTSKLVNLIDNGTISNSQARELFAH
                     IIETNEDPETKAKALNLMQVSDTYFILSIINEVINENLNVIDDIKAGKDRAMGFLVGQ
                     VMKKTKGKVNPALASKLMKQEIDKR"
     gene            complement(6007..7449)
                     /locus_tag="PK460_02535"
     CDS             complement(6007..7449)
                     /locus_tag="PK460_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOE54005.1"
                     /translation="MRYLGLTMAELHELYVSGKATPLDVVNEVIEALTKDKNNILEKT
                     MYDEARSMAQSLTKVEKDNLLWGIPFLVKDNIAMKGIETTGSSNILNGFIPLCNAEVI
                     DKLLAAKAIPVAKTTLDEFGMGGRGETGHKGITYNPYGKERTRIVGGSSSGSASGVAS
                     GYVPFAVGSDTGDSIRKPASFAGLVGFKPTWGEISRFGLFAFMPTLDHVGFFTRSVSE
                     ARVLFEKTRGYDKKDATSVMNVPFKTEKDATLKGKKVAVLEPIFALMKNKLLIKEFHN
                     LVDKLRQAGAVVDFVPFDKDLLAAIFPTYFILSCAEANVSNACLTGIGFGNKEEGASY
                     EEIVANTRGKGFSSRIKYRLMLGQYALKKENRNEYYDRAQKARRLIVEAVDKVLENYD
                     VIMLPAAPNHAPTVDKQSEPHSEIENNFMAIANFGGNPSLTLPYFLDEGLPLGVNLTG
                     KPFDDFNVLNIALKIEEITGLYNLHAGLKS"
     gene            complement(7451..7738)
                     /locus_tag="PK460_02540"
     CDS             complement(7451..7738)
                     /locus_tag="PK460_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54006.1"
                     /translation="MNKEEIRKLAAEILITLTPEEEATFAAEFAYFKELAKLLEALPN
                     IDEKEPMVFPFKVTQTYLKEDTPEESPGAKEILKNSKNAKDTFVVIPKVVK"
     gene            complement(7761..9752)
                     /gene="ligA"
                     /locus_tag="PK460_02545"
     CDS             complement(7761..9752)
                     /gene="ligA"
                     /locus_tag="PK460_02545"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOE54007.1"
                     /translation="MDYKARILYLREVLNKYNYEYYVLNNSSVSDAEYDSLMQELIAL
                     EKAHPDTFDKTSPSQRVGGFVASEFNKITHSSMMLSLANAFSADDLRDFDRRVRGVIK
                     KDVIDYVVELKIDGLAMSLMYIDGKLSYAATRGDGEVGEDVTSNIVTIKSIPLTLKEE
                     VSFEVRGEVFMPKKSWEELNAIREETGEALFANPRNAAAGSIRQLDSKIAASRKLDAF
                     WYFLVDPLSLNIKTHYDALDYLRTLGFKVNDKIKLCKGIEEVIRHIEKVTSQRHDFPY
                     EIDGIVVKVNDVTKYDELGYTAKTPRWATAYKFPPEEVETILEDIFFTVGRTGKITPN
                     AALKPVFVAGSKIQNATLHNAAFITDRDLKIGDHVFIRKAGDIIPEVLRAVPEKRTGA
                     EKDFVMIENCPVCLEPLTFKDPLHFCLNASCPARYEEGLIHFASRGAMDIEGLGERIV
                     EILFSQRLITNIADIYTLHTKREELMQIEGFGEKSVDNLLNAIEKSKEKSLEKLVFGL
                     GIKELGAKTSKILAEKYMTLDALSCATEAELLTIRDIGPVAASSIVSYFSNEQNLALI
                     TKLKEFGLNTDYLGSVKKDNTFFSGKTIVITGKFENYSRDELTALLENLGAKVAGSVS
                     KKTSLVVAGEDAGSKLTKAQELNIDILNEEQLLKLISEA"
     gene            complement(9755..11956)
                     /locus_tag="PK460_02550"
     CDS             complement(9755..11956)
                     /locus_tag="PK460_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE54008.1"
                     /translation="MNLYENLNSKQLEAVTTSGQYVRVIAGAGSGKTRVLTTRIAYLV
                     QEIGTPEDRIVGFTFTNKAAGEMTNRLKKYLERDNVRVRLSTFHSFGARLLREDIDVL
                     GYSRSFTIYDEEDTSSLIRNISVERGHERRGELTRDANNFIGAVKEKGLDAFTYKIRP
                     HGFVNEKEFVEIWKEYERRLKASNCLDFSDLISKSITILEEYRAIREKWQHRFDYILV
                     DEFQDTNDLQYKLIKLLMHQNTSLYVVGDPDQTIYTWRGANERIIMNFDSDFKNAETI
                     ILEENYRSTKYILDAANKLIAHNKNRVEKNLFTAKEKGEPVIVQYTESDSREVNWIYE
                     QIISLRLNVKDFSFRDVAILMRANYLTLPFERHFLHKNVPYRIYGGVRFYQRREIKDV
                     LAYLRILVNEKDDISFSRIVNVPRRGMGNVAMENLKIAALKNEYSLFETVHYVEDIGL
                     SSASQEQLKALMNAVDETRTKLQNDKLELSTVINDYIQKVGYYEHIKTEKDEDKQADM
                     RANVQVLIADIKQFMNDYPESTFNDYLENASLHSAQDEVEEGDYITLMTVHMAKGLEY
                     DYIFVSGLVEGVFPNNRALLDAGRAGEEEERRLCYVAFTRARKRLYLTMSSQYNFAIA
                     SGGRPSRFISEAGLGPPKIKEYHPKYGTPLKEGGVKQVKKEAPRYRANYAEYDYASDF
                     PYAVNDIVEHKTFGSGIVTAIFPTQTTIEVKFDTGEVKRLIASPKYFKEKK"
     gene            12032..12937
                     /locus_tag="PK460_02555"
     CDS             12032..12937
                     /locus_tag="PK460_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54009.1"
                     /translation="MKMNSLSRTHQLVLGALMGAINVIFALISSYLFAFSLIIMLFLP
                     LASIIVAINIDLKFYPVYLLGTLTLALVLNLGNIDNTLFFLLPILTSGLAFGLLIRHK
                     VPDILILLIVSGVNFLTLLITIPIINLIYDVNFLQVFASFIGFNNIEFGELVLPSILT
                     LLAVMQTLITLVIVTQDAAYFRLEINTEEWPYISLVNLGFSAIVTVLMFFNHGISLAL
                     LFVVILLSLYQIVHLFQKHTIFAWSTLLATIVFIIIGLALFENYTSLPYYFGIIIAVI
                     PVVISDILWLYISRKKSEAKNEGTI"
     gene            12921..13346
                     /locus_tag="PK460_02560"
     CDS             12921..13346
                     /locus_tag="PK460_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54010.1"
                     /translation="MKGLFKRLGQGILFILALPFGIAIFILFSIYAIITFFYTFFSAI
                     PAFFKGENVFGPTELDIAASTRLAEQKLAAQQPPPPPVVQPSTTINLFTVQQPGTEQV
                     PPPQVTYTQSDGVTYRRVTEQEVAALPEETIIDNSEEQK"
     gene            13343..14062
                     /locus_tag="PK460_02565"
     CDS             13343..14062
                     /locus_tag="PK460_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54011.1"
                     /translation="MILNPYILIKLSESEKRFLIVLFFLIVVVLFVCGLFYEGITRFM
                     ARQGEDIGRLMATQVESGYISSLKQFKRNSYRKSRLEFYRQFIKTFIMMIIWFAIYGT
                     VSIFYRHWLDLFDYTSEGFTTLLWKFDYANTPRTTFFGMSIISDWPPLLHKPFFSAKA
                     IPSYLLAVFGIVIIILFVGQVLSYLARTIFIIQHSKKMFTRDLRDYKLSDLSAAPAEL
                     QESGVNIPEVKPKEPLPPTNG"
     gene            complement(14052..14204)
                     /locus_tag="PK460_02570"
     CDS             complement(14052..14204)
                     /locus_tag="PK460_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54012.1"
                     /translation="MNTNKYGQDIPLTKEEIAIRGYKRRNLVLGIIIIILIIIIGIQI
                     GVMLTR"
     gene            complement(14214..15581)
                     /gene="asnS"
                     /locus_tag="PK460_02575"
     CDS             complement(14214..15581)
                     /gene="asnS"
                     /locus_tag="PK460_02575"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOE54013.1"
                     /translation="MLNVKALYTAYEKGELKAIPVKLQGWVKTNRNNGTIGFIEFNDG
                     SAFKGVQIVYEKNKIDNFAEVDKYRTGAALEVYGKVVLTPEMKQPFEVHADKVVLVGD
                     VAEDYPLQKKRHTPEFLREIAHLRPRTNTHTALFRLRSELSYALHKYLYDHEFVYVHT
                     PIITGNDAEGAGNVFNVVTDHKDPNFFFGKEASLTVSGQLHVESFALTFGKVYTFGPT
                     FRAEESNTTRHAAEFWMLEPELAFTDLNGNMDSIEEIIKYSVAYVLEKCSDEIDFFTK
                     FYDKTLKDRLEALVKSKVLRITYTKAVEILKEAQQKGVKFQFDNIFWGMDLASEHERY
                     LCEVYTNGPVFVTDYPKDIKAFYMRLNDDGKTVAACDLLVPGVGELVGGSQREDRYDV
                     LKAKMIEGGNLEDLEWYLDLRKYGGVPHSGYGIGFDRLLMYLTGVQNIRDVQPYARVF
                     GSIKY"
     gene            complement(15617..16630)
                     /gene="tsaD"
                     /locus_tag="PK460_02580"
     CDS             complement(15617..16630)
                     /gene="tsaD"
                     /locus_tag="PK460_02580"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOE54014.1"
                     /translation="MKERIILAIESSCDETAVAIMRGDKLLSNAVSTQISMHQKYGGV
                     MPELASRLHTENIVYTLDTALKDANIKLEEVDAFAVTRGPGLIGALHVGLQAAKTLAL
                     LYNKPLIPVHHLAAHIYASEFVKPLVFPLLAIVVSGGNTEFVVMKKHLDFTTIGETRD
                     DAIGECYDKIARVLNLGYPGGIPIDRLAKEGEATYKLPSPALTGYDFSFSGLKTHVIN
                     LVNNTKQRGEEINVPNLCASFQKVAIDAIIKKGLKAAQEYKVKQVVIAGGVSANSYLR
                     SEVLAKFPKIGVDPAIPPLWCCTDQAAMIAKLASYLYDEKVFADYDLGVDPNWEIEDF
                     REF"
     gene            complement(16627..17088)
                     /gene="rimI"
                     /locus_tag="PK460_02585"
     CDS             complement(16627..17088)
                     /gene="rimI"
                     /locus_tag="PK460_02585"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOE54015.1"
                     /translation="METKEKKLIIRPMTIDDVDAVDELAFAALPDPWPKYTYAQELKN
                     EFAYLFVLTYADVILGFSHFWITFDSATIVKFAVHPAAQGKGLGSLLMAHLIERVSML
                     EEVRAITLEVRTSNDKAMHLYLKYGFNVVTKKRKYYEDGEDAYYMIKVLKE"
     gene            complement(17093..17674)
                     /gene="tsaB"
                     /locus_tag="PK460_02590"
     CDS             complement(17093..17674)
                     /gene="tsaB"
                     /locus_tag="PK460_02590"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOE54016.1"
                     /translation="MITLLLDSSSHHLSVALAKGDKVLSFVEEKAFQTQSEIMVGRIN
                     KLLAQEKLTSDDIKRIVVASGPGSFTGVRIAVTIAKVWAYAKEVPLYAVSSLSVYRHE
                     TKPTICVLDARRERSFAGVYAQNNAILPDKIMQNSEILALAEKEGYLISGETKHLGIE
                     PAKFNRFENMLKSISEENRVKDIAAFKPLYLKG"
     gene            complement(17671..18132)
                     /gene="tsaE"
                     /locus_tag="PK460_02595"
     CDS             complement(17671..18132)
                     /gene="tsaE"
                     /locus_tag="PK460_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOE54017.1"
                     /translation="MKHEFISISREQTIALGKRLAALFNLGDVVLLTGDLGAGKTTIT
                     KGIASGLNITEKVNSPTFNILKIYLKGDKPLFHIDAYRLEDDKKDIGLDEYIGEAGIT
                     VIEWPAFIKHLLPEKALEINITHRDLTSRNITLKGEGHYADVVKVIAEEFK"
     gene            complement(18187..18720)
                     /locus_tag="PK460_02600"
     CDS             complement(18187..18720)
                     /locus_tag="PK460_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOE54018.1"
                     /translation="MSIKGKWIKIQSYKHDGNLHRFWRKSYVLEDNDEWLIVASRRTQ
                     VIEGNGRKWFTKEPAVTFFHKNDWFNVIAMLKTEGVVYYTNIASPTLIDYGMAKYIDY
                     DLDVKKFSDGTIKYLDKNEFLRNIENYQYSKELSAIVEYKQTQVVAMINEKTFPFDDD
                     EVRKLYNRFVEETKIKK"
     gene            complement(18777..19763)
                     /locus_tag="PK460_02605"
     CDS             complement(18777..19763)
                     /locus_tag="PK460_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54019.1"
                     /translation="MTEKEKMVEVVEETPAETKTPQAFVNEEISDDATNEQLELPALE
                     TMRNTFSKMYRKFRIFSYVSTAIVIAVIVVSYVWLLPINQWAGIVPIILVLVASLVYS
                     RWARNKLTDNVRKYMADYNGFVNELALSESKVNNYVFDFAGQLDKAAFTDARLLKDII
                     NSNSRNLVTYDLGRWHVKIADYAAYRRDGKQARAVFLGKLVTGTTEKEIDGRLVLYIK
                     PSEQKYDGGTGPDDLEDLELVVDKPEYKLYATNKELKKAISTKALEALLQVRPDNELA
                     DVSVALYKNKFAVTLTYSDALMVVPYKENVPVHAIKKYKEHLTALNKFIALL"
     gene            complement(19772..20413)
                     /locus_tag="PK460_02610"
     CDS             complement(19772..20413)
                     /locus_tag="PK460_02610"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE54020.1"
                     /translation="MMILNYTNKTNQELERLIKSNDPAAMHELARRLVQGEGIAEDNK
                     RGIELARQSEALGYAPADTVIGYCYFYGKGVEEDKQKGFAYFHSGAVKGHDAAQYNMG
                     IAYERGTVIPQDYALALEWYQRAADQKFAKAFYKLGYFHEHGMGVTKDVTKAFSYYEK
                     SANLGYVKAEVEVGKAHLSGRGLLRDLTKAREYLERAAAKGNRIAARLLRDIE"
     gene            complement(20458..21078)
                     /locus_tag="PK460_02615"
     CDS             complement(20458..21078)
                     /locus_tag="PK460_02615"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOE54021.1"
                     /translation="MKETTKKRRTLKPNRFFGRILTVRGITEMAIFIALALVFDLEFL
                     KFRLGEAQSFSITMLPLMIIALRFPLVDAFIGIGGIYGVITMLWDGYGLITYPLDYLL
                     AYGFLSIISLFQALVYGKHKHEIINYLYLALAVPAGVSGRIFFSTISGVVVYETPFVG
                     SLLYNLPAIGVSGIICGVLLMMLFPTLLRFRPQPFLQNEVEESKQQ"
     regulatory      complement(21119..21223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         6279 a         4366 c         3369 g         7217 t
ORIGIN      
        1 gagtgcattc ttttctggta agacatcaac aacgagtaag tttaagactg ttgttctttg
       61 cgctccatga tgtccaggca tttttgttcc gggaattacg cgttgaatac gaccaacgct
      121 agcttgcgaa ccaattccgc ggtgataacc agaaccgtgg gcggcatttc caagtttgaa
      181 gccatagcgt ttaatgacac cagtgtagcc tttccctttg cttgttccag ttacgtcgac
      241 gatatctcct tttttgaaga tgtcgacttt aatttcgcta ccaacttcgt agctaagcat
      301 ttcttcaccc tttaattcgt ataaaccgta ttttggagtg acacctgctt tttcgtatat
      361 gcctttaagt ggtttattta cccggctcgg ttttttatct tgatagccta cttggatggc
      421 ggcatagcca tctttttcca ctgttttcac ttgagtgaca acatttggta agacttcaac
      481 aactgtaacg gggtagattg tgccatcttc ggcgaatact tgggtcatac ccattttgcg
      541 gccaataatt gctttcatat ttctacctcc ttatagttta atatcgatgt cgacaccact
      601 tgggagttca aacttccgta agaattccac tgtgcgtttg tttggatcga cgatatcgat
      661 aagccgtttg tgtgttctaa tttcgaattg ctcacgcgca tctttgtact tgtggaccgc
      721 tcgtaacacc gtgtacacct cacgttgtgt tgggagcggg atggggccaa ctaccttggc
      781 gccactttcg actgcttgtt caacgatctt cgctactgct tcatcaagta atgcatgatc
      841 ataactttga agtcgaaccc ttatcttttg ttttcctgcc atgaattatt ttcctcctta
      901 catctacagg cgctaatggt cctcagctac gaattccccg attacacttc atgacaacgc
      961 ttgtaggtgt atcggcaacc tcgcatttca acgcgaccat taacttgtta aataatacta
     1021 aaagcataat ataatgtcaa ccataaacga taaaaaatat atatttttat atataaggtc
     1081 acaaaatctt gtgaaaaacc aaatttcaaa cttttgaact ttttaccgaa aatcgtgaaa
     1141 aaaaccactt ttgcaagcgg ttccattttt tcttaaatac tgctgttttt gtccaaaaat
     1201 cgtgaaaaat caacggtttt cggtgtcttt tttattattt cttgctaaaa cggtgtcgcg
     1261 ataagagtat tcacaaccac aatattcttg ctgataaagg ttatattctt ttttcatttc
     1321 acggacacgc aataatccgg cctttttctt aaaatcacta taaaaatatt taatatttgg
     1381 gtagcgaggc gcaatctttt tagcaattgc attgatgact tgtgagttct tttgtcgcga
     1441 gatagtaagc gtcgtagtgg cgtaatcaaa tccgttttca ctaccatact ttaaagcttc
     1501 tacaatacgt ttttcataac aaagatgaca gcgtttaccg ccttcttttt cactagcgaa
     1561 cactcttaat tccttcataa aggcttcgtg atcatatggt aaaaacacga ctttgacatc
     1621 ttgattattt ttaatattaa attctcttac gaatctttca acttcgtcgc gtcttttctt
     1681 aaactcagct ttgggataga tgttgctatt ttgatacaca attgtcacag caaaaaatgg
     1741 tgttaaaaat tctagggtgt gactagaaca cggcgcacag caagcatgga gcgctagttt
     1801 tggtctaagc ccttgatctt tgaaattatt aacttctgca atcgaaatgt tgtaataatt
     1861 aatcttctcc atatatatac attacatcac caaaagagaa aaagcgatag cgttctttaa
     1921 ttgcgtggca ataagccgcg agtgtatttt cacgccccgc aaaagcacta actaacataa
     1981 ttagtgtaga tttaggtaaa tgaaaattcg taattaacgc gctgatgact ttaaatttat
     2041 aacctggagt aataaataaa tttgttgctt cactcctcgc tacaatcttt tgatatttat
     2101 taaaattagc ttctagtgtt cttgttgcgg ttgtgccaac actgatgatt cttcgttttt
     2161 cttcaatcgc tttatttatt gttgcggccg cttcttcgct aatttcataa gtttcgtaat
     2221 gcatcttatg atctttagta tcactaacac tgaccggttt aaaagtacct aagccaatat
     2281 gcagtgtaat ttcgacgact atcactcctt tagtttttaa ctcatctaat atttccggcg
     2341 taaagtgaaa gcctgctgtt ggtgctgccg cactaccagg cgctttagcg tacaccgttt
     2401 gataacgcgc atatttttca cttttatctt caatataagg cgggagcggg gttgtcccaa
     2461 ccctttctaa gacttctaaa aagacaccct catacttaaa ctttacaatg tgaatgcctt
     2521 ctcctttttt ctcgcggcat tccattagaa gctcgccgtc accaaaagaa acaacagttc
     2581 caactttaac ggctttagca ttaccaatta acacttcata ttcatcgtct tttagtgctt
     2641 ttaataacac aacctcaatc tttgctcctg ttggaacttt aatgcctcta agtctagcgg
     2701 gaataacttt tgtattatta ataactaaca catcaccatg cttaaaatac ttagtaatat
     2761 cataaaaatg ttcatctttt atttcatttt tatctttgtt aaacgctaaa agacgtgcat
     2821 tttgccgtaa aagagaagga tattgcgcaa ttagttcttt tggtaaatca aaatcaaaaa
     2881 gattaacatc cattagaaac ctcttttatc gccaaacttt tttaagacat cactcttaaa
     2941 atctaaaaag cgattttctt taatcgccac acgcacatct tccataagtt taattaaaaa
     3001 gcgtaagtta tgaattgaga gtaagcgctg cgctaaagct tcttcactaa catataaatg
     3061 gcgtaaataa gcacgcgtgt aattcttaca tgtatagcaa tcgcactctt cttcaattgg
     3121 gcggaagtca tctttaaatt cagcgcgccg gatattaagt ctgccttttc ttgtatagag
     3181 cgaaccgtga cgcgcaatac gcgttggtag tacacaatca aacatatcaa caccgcgcgc
     3241 tacaccttgt aagatcatat caattgaacc aatccccatt aagtaacgcg gtttgtcttt
     3301 aggtaaataa gggacggaac tttccaccat ctttataaag acatcttttg gttcaccgat
     3361 gcttgttcca ccaattgcgt aaccaggaaa cccaattttt actgtttcca tcgcactata
     3421 tttgcgtaag tcggggtatt ctccgccttg tacaatacca aataaagctt ggtcttcacg
     3481 cttatgcgct gcttttccgc gcttagccca gcgtaaagta cgttcagtac ttgcttttac
     3541 atattcataa tctgcgggat aatgaataca ttcatcaaaa ctcataataa tatccgcacc
     3601 aatttcttct tgcatcctaa ttgcttcttc tggtgagaag aaaagttcgc gtccatctaa
     3661 atgtgattta aaggtcacac cttcttcgct aatcttacga ttatcggcta aagagaatac
     3721 ttgaaagccg cctgaatcag taagaattgg tccgtgatga ttcataaaag agtggattcc
     3781 accagccttt tttaaaatat cagtgcctgg tctaagtgat aagtgataag tattcgccaa
     3841 gatgactccg ctgcctaagg caacaatttc ttcaggagta attgttttaa cattagcaag
     3901 cgtacctacc ggcataaaca tcggcacttc aactttgccg tgcggtgtat gtaaaaaacc
     3961 gtatcgcgca ccgctttgtg tgcattcatg aataatttct agataaacgg gtggtttttt
     4021 agccatcttt ttcctctttt ctttgttcag ccataatgag atttagaaat gcaggttgct
     4081 tcgctaggag cgaaaacaca gcatgcccta ataagagtac aacaaattca ccaattgcga
     4141 caagtcccat tgtagcaaat aatggtaaat cgagaataat gtgtaacatt agtccgacga
     4201 caacagcatt aattattgtc ggccatatta caccccaata tagtctttta ctaaaagcaa
     4261 ttaacactcc acttaataaa gtagcaagcg tcccaaagat aacatcccac ggttgaagca
     4321 gactatgcaa attagcgagc aaacacccga gtgtaacacc aatgacaaag tcttttctaa
     4381 aaaatattaa aaataataat atttctgaga tccgaaattg cacatcatta aaagcaaatc
     4441 cgccaagaat aattgttaat acataataaa tggcagcaat cagcgcatta tctgtaatct
     4501 gccgaataat ctttttcttc ttcatatttt ctccttgttt tgatggtggt ggttaggcaa
     4561 ggaacacctc aactccgctt gcttagcgct tatcaatttc ttgcttcatc agtttgctag
     4621 caagagcggg attaactttt ccttttgtct ttttcataac ttgtccgact aagaacccca
     4681 tagcccgatc tttacctgct ttaatgtcat caattacatt taaattttca ttaatcactt
     4741 cgttaataat actaaggata aagtaagtat cactaacttg cattaaatta agtgctttcg
     4801 cttttgtttc ggggtcttca ttagtttcaa taatgtgcgc aaataattca cgcgcttggc
     4861 tatttgaaat cgtgccatta tctattaaat tcactaattt agaggtgttt tgcggggata
     4921 atgctacact tttagcgttt ttcccttctt tatttaagta cgaactaaat tcaccaataa
     4981 cccaattaat tagtgtctta tagtttttaa catccctcgc tgcttcatca aaataacttg
     5041 ttaaatcttt atcttgtagc aagatattta cttcatgagc gttaagttta aattcttcaa
     5101 aatagcgttc acgctttacg ctcggtaact ctttcatttc cctaattgtt gcatcaataa
     5161 attccttgct taaagaaatt ggtaaaatat ttccttcagg aatatatcga taatcaacgc
     5221 tttcatcttt tttacgcatt gtaattgtat ctttaagggc atcgtcatac cgccgtgtct
     5281 cactttcaac ttcaagccct ttatcaagta attcactttg ccgtttaatt tctgtttcaa
     5341 ttgctttgcc aatattagaa attgagttca tatttttaat ttctacttta ttaccaaact
     5401 ttgaaactcc cacagggcga agtgaaatat taacatcaca gcgtagtgat ccttcttcca
     5461 tttttccgtc acttatttcg aggaatgtga cgatttcacg cactgcttcc acaaacgcca
     5521 ttgcttcgtc agcacttctt aagtctggtt ctgtgacaag ttccacaagc ggattaccag
     5581 cgcggttata gtcaataagt gtaacttcac caaggtgaat ctgtttagcg gtatcttctt
     5641 ccatgtggat gcgttcaatc cgaatgcgtt ttttagtgtc attaacataa atatctaaat
     5701 aaccattact accaattggc cgtctttgtt gggtgatttg ataacctttt ggtaaatcac
     5761 tataaaaata atttttgcga tcaaaatgta attcatcatc aatttccata tttaaagcgt
     5821 gacctaacat aattgcattt tgcactgcct ttttatttac cgttggtaat gtgccaggaa
     5881 aagccatatc aacaacacta acattgctgt tcgcaggtgc atcaaaaaga ctagaagcgc
     5941 tagaaaacat ctttgtgttt gtttttgttt caatatgaat ttctaaacca ataattactt
     6001 cgtatttcat gattttaacc ccgcgtgtaa gttgtaaagc ccggttattt cttcgatttt
     6061 aagtgcaata tttaacacat taaagtcatc aaacggttta ccagttaaat taacaccaag
     6121 cggcaaacct tcatctaaaa agtaaggaag ggtaagtgaa ggattaccac caaaattagc
     6181 aatagccata aagttatttt cgatttcact atgtggttca ctttgtttat ctactgttgg
     6241 agcgtgattc ggtgcagcgg ggagcatgat gacatcataa ttttctaata ctttatccac
     6301 tgcttcaaca attaagcgac gtgctttttg ggcacggtca taatattcat tcctattttc
     6361 tttcttcagc gcatattgtc ctaacattaa acggtattta atccgtgaag aaaagccttt
     6421 gccccttgta ttagcaacga tctcttcata acttgcgcct tcttctttat taccaaagcc
     6481 aatccctgtt aaacatgcgt tacttacatt agcttcggca cagcttaaga taaagtaagt
     6541 tggaaatatc gccgctagca ggtctttatc aaacggaaca aagtccacaa ctgctcccgc
     6601 ttggcgaagt ttatcgacta gattatgaaa ttctttaatt aataatttat ttttcattaa
     6661 cgcaaaaata ggttctaata ctgcaacttt tttgcccttt aaagttgcat ctttctctgt
     6721 tttaaaggga acattcatta ctgaagttgc atctttttta tcgtaacccc tcgtcttttc
     6781 aaaaagtaca cgcgcttcac taacactgcg ggtaaaaaat ccaacatggt ctaaagttgg
     6841 cataaatgcg aatagaccaa aacgcgaaat ttcgccccat gttggtttaa agccaacgag
     6901 tccagcaaag cttgcgggct tacggattga gtctcccgta tcactaccaa ccgcaaaagg
     6961 aacataccca ctagcgaccc cactagcgct accgctacta gaaccaccga caattcgtgt
     7021 tctttcttta ccatacggat tatatgttat ccctttgtga ccagtttcac cgcggccacc
     7081 catcccaaat tcatcaagtg ttgtttttgc aacaggaatt gctttagcag ctaaaagttt
     7141 atcaataact tcagcattgc aaagcggtat aaagccgtta agtatattac tactgcctgt
     7201 tgtttcaatg cctttcattg cgatattatc tttgactaaa aaaggaattc cccatagtaa
     7261 attatctttt tcaactttag taagactttg tgccatactg cgtgcttcgt catacattgt
     7321 tttttctaaa atgttgtttt tatcttttgt taaggcttca attacttcat taacaacatc
     7381 taatggtgtt gctttacccg acacatataa ttcatgaagt tccgccattg ttaaacctag
     7441 gtagcgcata ttatttcacc acctttggga taacgacaaa agtgtctttt gcgtttttac
     7501 tattttttaa tatctcttta gcgccaggac tttcttccgg cgtgtcttct tttaaatatg
     7561 tttgtgttac tttaaaaggg aacaccattg gttctttttc atcaatatta ggtaaagctt
     7621 ctaaaagctt cgctaattct ttaaagtaag caaattctgc ggcaaaagtg gcttcttctt
     7681 caggagtaag cgttattaat atctctgctg ctaactttct tatttcttct ttattcataa
     7741 ttatcctcgc tagtaatata tcatgcttcg ctaattaatt ttagcaattg ttcttcgttt
     7801 aatatatcaa tattaagttc ttgcgctttt gttaatttgc taccagcgtc ttcgccagca
     7861 acaacaagtg aagttttttt actaacactg cccgctactt tagcgcctaa attttctaag
     7921 agagcggtta attcgtcacg tgaataattt tcaaacttac ccgtaatgac aattgtctta
     7981 ccagagaaga aagtattatc ttttttaacg gaccctagat aatccgtgtt taagccgaac
     8041 tcttttagtt tagtaattaa cgctaaattt tgttcattac taaagtaaga cacaattgaa
     8101 gaagcagcaa ctggaccgat atctctaatt gttaaaagtt ctgcttcagt agcgcaacta
     8161 agtgcgtcta aagtcatata cttctcggct agaatcttac ttgttttcgc tcctaattct
     8221 ttaatcccta aaccaaacac taatttttct aaagactttt ctttactttt ttcaatagcg
     8281 tttaataagt tatcaactga tttttcacca aagccttcaa tctgcattaa ttcttctctt
     8341 ttagtgtgca gtgtataaat atcagcaata ttagtaatta atctttgtga gaaaagaatt
     8401 tctacaattc tttcgccgag cccctcaata tccattgcgc cgcgactcgc aaaatgaatt
     8461 aagccttctt cataacgcgc aggacacgac gcatttaaac aaaaatgcag cgggtcttta
     8521 aaggtaagcg gttctaaaca aaccggacaa ttttcaatca ttacaaaatc tttttcggca
     8581 cccgttcttt tttctggtac agcgcgcaat acttctggaa taatatcacc agctttacga
     8641 ataaaaacat ggtcacctat ttttaagtca cgatcagtga taaacgctgc attatgtaaa
     8701 gtagcgtttt gaatctttga acccgcgaca aatactggct taagtgcagc gtttggtgtt
     8761 attttacctg ttcttccaac cgtaaagaaa atatcttcaa gtattgtttc aacttcttcg
     8821 ggcgggaatt tataagcagt agcccaccgt ggcgttttag cggtataacc aagttcgtca
     8881 tactttgtaa cgtcattaac tttcacgaca ataccgtcga tttcataagg gaaatcatgt
     8941 cgttgcgatg ttactttttc aatatgccga attacttctt caatcccttt acataattta
     9001 attttgtcat ttaccttaaa tcctaaagtg cgtaaataat caagagcatc ataatgcgtt
     9061 tttatattta gtgaaagcgg atcaactaag aaataccaaa aagcatcaag cttccgacta
     9121 gcagcaattt tagaatcgag ttgacgaatt gagcccgcgg cggcgttacg cggattagca
     9181 aaaagtgctt cacccgtttc ttcacgaata gcatttaatt cttcccaaga ttttttaggc
     9241 ataaatactt cgccacgcac ttcaaaacta acttcttctt taagtgttaa aggaatactt
     9301 ttaattgtga caatatttga agtaacatct tccccaactt caccgtctcc tctagttgcc
     9361 gcataactta actttccgtc gatatacatt aagctcatcg ctaagccgtc aatctttaac
     9421 tctacgacat aatcaatgac atctttttta ataacaccgc ggacacgacg atcaaaatca
     9481 cgtaagtcat cagcactaaa ggcattcgca agtgaaagca tcattgaact atgtgtaatc
     9541 ttattaaatt cacttgcaac aaagccacca actctttggc tcggacttgt tttatcaaaa
     9601 gtatcgggat gcgctttttc aagggcaatt aattcttgca ttaaactatc atattcagca
     9661 tcactaacac tcgaattatt aaggacataa tattcatagt tatacttatt taaaacttcg
     9721 cgtaaataaa gaattcttgc tttataatcc atctttattt tttctcctta aagtattttg
     9781 ggctagcgat tagtcgcttt acttcgccag tatcaaactt aacttcaatt gttgtttgcg
     9841 ttggaaaaat ggcagtaaca attcctgatc caaatgtttt atgttccaca atatcgttaa
     9901 ctgcataagg aaaatcacta gcgtaatcgt attctgcata attagcgcga taacgcggcg
     9961 cttctttttt aacttgttta acaccacctt ctttgagcgg tgtcccgtat tttggatgat
    10021 attctttaat cttcggtggt cctaaccccg cttcactaat aaagcgtgat ggtctcccgc
    10081 cagaagcaat cgcaaagtta tattgcgaac tcattgttaa ataaagccgt ttacgcgctc
    10141 tagtaaaagc aacgtaacat agtcttcttt cttcctcttc accagcgcga ccagcatcaa
    10201 gcaacgctcg gttatttgga aagacacctt caacaagtcc tgaaacaaaa atataatcat
    10261 actcaagacc ttttgccata tgcacagtca taagcgtaat ataatcacct tcttcaactt
    10321 catcttgggc gctatgtaaa ctagcgttct ctaaataatc attaaaggtt gattcaggat
    10381 aatcattcat aaattgtttg atgtcggcaa ttaagacttg gacgttagca cgcatatctg
    10441 cttgtttatc ttcatctttt tcggttttaa tatgttcgta gtaaccaact ttttgaatat
    10501 agtcattaat aacagtactt agttcaagtt tgtcattttg taattttgtt cgtgtctcat
    10561 ccacagcatt cattaacgct tttaattgtt cttgtgaagc acttgataag ccaatatctt
    10621 caacataatg cactgtttca aatagtgaat attcattctt taacgcagca attttaaggt
    10681 tttccatcgc tacattaccc atgccacggc gcgggacatt aacgattctg ctaaaagaaa
    10741 tatcatcttt ttcattaact aaaatccgca gatacgctaa aacatcttta atctcgcgtc
    10801 tttgatagaa acgcacgcca ccataaatac ggtacggaac attcttatgt aaaaagtgcc
    10861 gttcaaatgg tagtgttaag taattagcgc gcataagaat tgcgacatca cgaaacgaaa
    10921 aatctttgac gtttaagcgc aatgaaataa tttgttcata aatccaatta acttcgcgtg
    10981 aatcgctttc tgtatattga acaatcactg gttcaccttt ttctttagcg gtaaaaagat
    11041 ttttctccac tctatttttg ttatgagcaa taagtttatt tgctgcatcc aaaatatatt
    11101 tagtggaacg atagttttct tctagaataa tcgtttcggc atttttaaaa tcactatcaa
    11161 aattcataat gattcgctcg ttagcgccac gccatgtata aattgtttga tcgggatcac
    11221 ccacgacata gagtgatgta ttttggtgca ttaaaagttt aatgagttta tattgcaaat
    11281 cattagtgtc ttgaaattca tcaacgagaa tataatcaaa gcgatgttgc catttttctc
    11341 taattgctct atattcctct aaaatcgtaa ttgatttact aattagatca ctaaaatcaa
    11401 gacagtttga cgctttaagt cttcgttcat attctttcca tatttcaacg aattcttttt
    11461 cattaacaaa gccgtgcggt ctaattttat aagtaaaagc atctaagcct ttttctttaa
    11521 ccgccccaat aaaattattt gcgtcacgcg ttaattcgcc gcggcgttca tgtcctcttt
    11581 ctacacttat attgcgaatt aaggaacttg tatcttcttc gtcatatatt gtaaagctgc
    11641 gtgaatagcc taaaacatca atgtcttcac gcagtagtct tgcaccaaaa gagtgaaatg
    11701 ttgaaagacg cacacgaaca ttatcgcgtt ctaaatactt ttttaagcga tttgtcattt
    11761 cgccagcggc tttatttgta aaagtaaaac cgacaatgcg gtcttccgga gtacctattt
    11821 cttgcactaa ataagcaata cgagtagtga gtactcgcgt tttaccactt cccgcaccag
    11881 caataacacg cacatattgc ccgcttgtag taacagcttc taactgtttt gaattaagat
    11941 tttcatacaa attcatatct aaaatattat aacataatat aaggtgaaat tactcccgta
    12001 gtgaagccct tttttatgtt aaaatatgac tatgaaaatg aactcattat cacgcacaca
    12061 tcaactcgtt ttaggcgccc tcatgggtgc gataaatgta atatttgcac ttatcagcag
    12121 ttatttattt gcttttagtt taattattat gcttttttta ccgctggcga gtattattgt
    12181 cgcaattaat attgatttaa aattttatcc tgtttattta ctaggtacgc ttaccttagc
    12241 gcttgtatta aatcttggta atattgataa cacacttttt ttcttattac cgatattaac
    12301 aagcggatta gcctttgggc ttttaattag acacaaagta cccgacatct taattttatt
    12361 aatcgttagc ggtgtgaact ttcttacact tttaattaca ataccgatta ttaatttaat
    12421 ttatgatgtg aatttcttac aagtgttcgc tagttttatc ggttttaata atattgaatt
    12481 tggtgaatta gtgttgcctt caatcttaac cttattagcg gttatgcaaa cactcattac
    12541 gcttgtgatt gtaacgcaag acgctgctta ttttagatta gaaattaata cggaagagtg
    12601 gccttatatt agtttagtta atttaggatt tagcgctatt gttacggtat taatgttttt
    12661 taatcacggg atttcgcttg cgcttttgtt cgttgttatt ttgctttcac tctatcaaat
    12721 cgtgcatctt ttccaaaaac acaccatttt tgcatggtca acattacttg cgacaattgt
    12781 gtttattatt attggtttag cattatttga aaattatact tcgcttcctt attattttgg
    12841 tattattatt gcggtaattc cagttgtgat tagcgacatt ttgtggttat atattagtag
    12901 aaagaaaagc gaggcaaaaa atgaagggac tatttaaaag attaggtcaa ggaattcttt
    12961 ttatcctagc gctaccattt gggatagcga tctttatcct tttttcaatt tacgccatta
    13021 ttactttttt ctatactttc ttttctgcta ttccagcatt ctttaaaggt gaaaatgtct
    13081 tcggtccaac cgaattagat attgccgcta gtacacgctt agccgaacaa aaactagcag
    13141 cgcaacaacc accaccgcca ccagtcgtgc aaccttcaac aactattaac ttatttacag
    13201 tacaacaacc cggcaccgaa caagtgccac caccacaagt cacttatacc caaagtgatg
    13261 gtgttactta tcgccgtgtt actgaacaag aagttgcagc acttcctgaa gaaactatta
    13321 ttgataatag cgaggagcaa aaatgatttt aaatccgtat atattaatta agcttagtga
    13381 aagtgaaaaa aggtttttaa tcgttctctt tttcctaatt gtcgttgtgc tttttgtatg
    13441 cggactattt tatgaaggta ttacgcgctt tatggcacgc caaggtgaag atattggcag
    13501 actcatggca acacaggtgg aatcgggtta tatatcttca cttaaacaat ttaaaagaaa
    13561 ctcttaccgt aaatcacgct tagaatttta tcgtcaattt ataaaaacat tcattatgat
    13621 gattatctgg tttgcgattt atggtactgt tagtatcttt taccgtcact ggttagattt
    13681 atttgattac actagtgaag gttttactac cctcttatgg aaatttgatt acgctaatac
    13741 gccacgcact actttctttg gcatgagcat tattagtgac tggccacctc ttcttcataa
    13801 accattcttc agcgctaaag caattccaag ctacctttta gcggtgttcg gtattgtgat
    13861 tatcatttta tttgttggcc aagtactttc ctacttagcg cgtacgatct ttattattca
    13921 acatagtaaa aagatgttta ctcgcgattt acgtgattat aaattaagtg atttaagtgc
    13981 cgctcctgct gagttacaag aaagcggtgt gaatattcct gaagttaaac ctaaagagcc
    14041 cttaccaccg actaacgggt aagcattaca ccaatttgaa ttccaataat aattattaaa
    14101 ataataataa tgatacctaa aacgaggttc cgtcttttat aaccacggat tgctatttct
    14161 tcttttgtta aggggatatc ttgtccgtat ttatttgtat tcactttgca cctctaatat
    14221 ttaattgagc caaatacccg agcgtaaggt tgaacatcac gaatattttg cactccagtt
    14281 aagtacatca ataagcggtc aaacccaata ccgtatccag aatgggggac accaccatat
    14341 ttacgtaagt ctaaatacca ttctaagtct tcaaggttac cgccttcaat catctttgct
    14401 tttaaaacat cgtatctatc ttctctttgt gaaccaccaa ctagttcacc aacacctggc
    14461 actaataagt cacacgctgc gactgtttta ccatcatcat ttaggcgcat ataaaatgct
    14521 ttaatgtctt taggataatc agtaacaaac actggaccat tcgtataaac ttcacaaaga
    14581 taacgttcat gttcacttgc taaatccata ccccagaaaa tattgtcaaa ttggaattta
    14641 acgccttttt gctgggcttc ttttaatatt tcaacagctt tagtgtatgt aatacgtaat
    14701 actttacttt tcactaatgc ttctaaacgg tcttttaatg tcttatcata aaatttcgta
    14761 aagaaatcaa tttcatcgga acatttttct aatacataag caacagaata cttaataatt
    14821 tcttcaattg aatccatatt accatttaaa tcggtgaacg ctagttcggg ttcaagcatc
    14881 caaaactccg ctgcgtgccg ggtagtatta ctttcttcag cacggaatgt tggtccaaag
    14941 gtataaactt taccaaatgt taacgcaaat gattcaacat ggagttggcc tgagacagta
    15001 agtgaagctt ctttaccaaa aaagaagttt ggatctttat gatcagtaac aacattaaag
    15061 acattacccg ctccttcagc gtcgtttccg gtaataattg gtgtatgaac atagacaaat
    15121 tcatggtcgt ataagtattt atgaagcgca tatgatagtt cactccggag tcggaataaa
    15181 gcagtgtgtg tattggtccg cggtcttaag tgcgcaatct ctcttaaaaa ttcaggagta
    15241 tgccgtttct tttgtaacgg ataatcttct gcaacgtcac caacaagaac aactttatca
    15301 gcgtgtactt caaagggttg cttcatttct ggagttaaca caacttttcc gtaaacttca
    15361 agcgccgctc cagtgcgata tttatcaact tcagcaaagt tatcaatctt atttttttca
    15421 taaactattt gtacaccctt aaaggcacta ccatcattaa attcaataaa accaatcgtg
    15481 ccattattac gatttgtttt gacccatcct tgtaatttaa caggaatcgc tttaagttcg
    15541 cctttttcat aggcggtata tagtgctttt acatttaaca tattgttacc tctttgactt
    15601 ctttattaat tatatcttaa aactctctaa aatcttcaat ttcccaatta ggatcaacac
    15661 ctaaatcata atccgcaaaa actttttcat catataagta tgatgctagc ttagcaatca
    15721 tcgctgcttg gtcagtacaa caccatagtg gcggtattgc gggatcaact ccaattttcg
    15781 ggaattttgc tagtacttcg ctccgtaaat atgagtttgc gctcacgcca ccagcaatta
    15841 caacttgttt gactttatat tcttgtgctg cttttaatcc ttttttaata atggcatcaa
    15901 ttgcgacttt ttgaaatgat gcacataagt tcggcacatt tatttcttcg cctctttgtt
    15961 tagtattatt aactaaatta attacatgtg tctttaaccc ggaaaaagaa aagtcgtaac
    16021 cagttaaggc aggacttggt aatttgtaag ttgcttcacc ttcttttgct aaacggtcaa
    16081 ttgggatacc gcccggataa cctaaattta agacacgcgc tattttgtca tagcactctc
    16141 caatcgcgtc gtcacgcgtt tccccaattg ttgtaaagtc taaatgtttt ttcatcacaa
    16201 caaattcagt gttaccgcca cttacaacaa tagcaagcag tggaaacaca agcggtttaa
    16261 caaattcact agcgtaaata tgcgctgcta agtggtgaac agggatcagc ggtttgttgt
    16321 aaaggagcgc taatgtttta gcagcttgta acccaacgtg gagcgcccca ataagtccag
    16381 gtccgcgagt caccgcaaaa gcatcaactt cttcaagctt aatattcgca tcttttaggg
    16441 cagtatctaa tgtataaaca atgttttcgg tgtgaagtct tgaggctaat tcaggcataa
    16501 ctccgccata cttttgatgc atcgaaattt gtgtactaac agcgttactt aaaagtttgt
    16561 caccacgcat aatagcaaca gcggtttcat cacaactaga ttcaattgct aatattattc
    16621 gttctttcat tcttttagta cctttatcat ataataagca tcttcaccgt cttcataata
    16681 cttccgtttc ttagtcacaa cattaaatcc gtattttaaa taaagatgca ttgctttatc
    16741 gttactcgtt cttacttcta atgtaatagc tcttacttct tcaagcattg acactctttc
    16801 aatgagatgc gccattagta aacttcctag tcccttgcct tgcgccgcgg gatgcacagc
    16861 aaatttaaca atggtggcac tatcaaacgt aatccaaaaa tgggagaatc caagaatcac
    16921 atcagcgtaa gttaagacaa atagatatgc aaattcattt tttaattctt gggcataagt
    16981 atatttaggc cacggatcag ggagtgccgc aaacgctagt tcatcaacag cgtcaacatc
    17041 gtctatagtc attggacgaa taataagttt cttttctttt gtttccatct tattatccct
    17101 ttaaataaag cggcttaaac gctgctatgt ccttaactct attttcttca ctaatgcttt
    17161 taagcatatt ttcaaaacga ttaaactttg cgggttcaat tcctaaatgt tttgtttcgc
    17221 ctgaaattaa gtaaccttcc ttttccgcta gtgctaatat ttcgctgttt tgcattattt
    17281 tgtccggcaa aatggcatta ttttgcgcgt aaaccccagc aaaagaacgc tctcttcttg
    17341 catcaagtac acaaatggtc ggttttgttt catggcgata aacacttaaa ctagaaacag
    17401 cataaagtgg gacctcttta gcgtacgccc atactttggc aattgttaca gcaattctta
    17461 ccccagtaaa agagcctgga ccacttgcga ccacgattct tttaatgtca tcacttgtta
    17521 atttttcctg cgctaatagt ttattaattc ttccaaccat tatttcactt tgggtttgaa
    17581 aagccttttc ttcaacaaaa cttaaaactt tgtcgccctt tgcaagcgca acacttaaat
    17641 gatgactcga actatctaaa agtaatgtaa tcatttgaac tcctcagcga taacttttac
    17701 cacgtccgca taatgacctt ccccttttaa tgtaatgttt cttgaagtta aatcgcgatg
    17761 cgtaatatta atttcaagtg ccttttcggg taaaagatgc ttaataaatg ccggccactc
    17821 aataacagta atacctgctt caccaatata ttcatctagt ccaatatctt ttttatcatc
    17881 ttctaaacga taagcatcaa tatgaaataa tggtttatca ccttttaaat aaatcttaag
    17941 aatattaaag gttggactat taactttttc tgtaatgttt aaaccgctcg ctattccttt
    18001 tgtaatcgtt gttttaccag cccctaagtc accagtaagc agcacgacat cacctaaatt
    18061 aaataatgcc gctagtcttt tacctagcgc tattgtttgt tctctactaa tacttataaa
    18121 ttcatgtttc atagacgcca aaattatacc atgattagtt aggtttcaat ttataattaa
    18181 aatatattat tttttaatct ttgtttcttc aacaaatcga ttataaagtt tccgaacttc
    18241 atcatcatca aacggaaacg ttttttcatt tatcatcgct acgacttgcg tttgtttata
    18301 ttcaacaatt gcggataatt ctttggaata ttgatagttt tcaatattgc gcaaaaattc
    18361 atttttatca aggtatttaa tcgtaccatc actaaatttc ttcacatcta aatcgtaatc
    18421 aatatacttc gccatcccgt aatcaatcag tgtcggtgaa gctatatttg tgtaataaac
    18481 aacaccttca gttttaagca tagcaatcac attaaaccag tcgtttttat gaaaaaaggt
    18541 aaccgctggt tcttttgtaa accacttgcg accgttacct tcaattactt gtgtacggcg
    18601 tgaagcgacg attaaccatt catcgttatc ttctaaaaca taagacttac gccaaaaacg
    18661 atgtaaatta ccatcgtgtt tatagctttg gattttaatc catttacctt tgatactcat
    18721 aactaactca actctcgatt gttagttaga ttttaacata aaaactgatg tttttactat
    18781 agtaaagcga taaatttgtt taaagctgtt aaatgctctt tatatttctt aattgcgtgc
    18841 actggcacat tctctttata tggcacaacc attaaagcat cactataagt aagtgtaact
    18901 gcaaatttat ttttatataa tgcgacactt acatcggcga gttcgttatc aggacgaact
    18961 tgtaacagtg cttcaagtgc ttttgtagaa atagcttttt taagttcttt attcgttgca
    19021 tataatttgt attctggttt atccacgact agttctaagt cttcaagatc atctggacca
    19081 gtgccaccat catatttttg ttcacttggt ttaatataaa gcacaagtcg tccatcaatt
    19141 tctttttcag tcgttcctgt cactaatttt cctaagaaaa cagcacgtgc ttgcttacca
    19201 tcgcgtctat aagcggcgta atcagcaatt ttgacatgcc agcgtcctaa gtcataagta
    19261 actaagttac gagaattaga attaataatg tctttaagta atcgagcgtc agtaaacgct
    19321 gctttatcta attgaccagc aaaatcaaag acataattat taacttttga ttcactaagc
    19381 gcaagttcat tcacaaagcc attgtaatca gccatatatt ttcgcacatt gtcagtaagt
    19441 ttgttacgcg cccagcgtga atacactaaa gaagcaacaa gtactaaaat aattggaaca
    19501 ataccagccc actgattaat tggtagtaac cacacataag aaacaactat cacagcaata
    19561 acaattgcag ttgacacata actaaaaata cggaactttc gatacatttt cgaaaaagta
    19621 ttccgcattg tttctaatgc tggtaattct aattgttcgt tagtagcgtc atcactaatt
    19681 tcttcattaa caaatgcttg tggagtttta gtttctgcag gagtttcctc cactacctcc
    19741 accatctttt ctttttctgt catataattc tctattctat gtcacgtaat aagcgcgcag
    19801 caatacggtt tcctttcgcg gcagcacgct ctaaatattc acgggctttc gttagatctc
    19861 gtagtagtcc tcggcctgat aaatgagctt taccaacttc cacttcagcc ttaacataac
    19921 cgagatttgc agacttttcg tagtaactaa atgcttttgt cacatctttt gttacaccca
    19981 tcccgtgttc atggaagtaa cctagtttgt agaaagcttt ggcaaatttt tgatcagccg
    20041 ctctttgata ccattcaagc gctaaagcgt aatcttgcgg aataacagtc ccgcgctcgt
    20101 aagctatccc catattatat tgagcagcgt cgtgaccttt aactgctccg ctatggaagt
    20161 aagcaaatcc tttttgttta tcttcttcaa cacctttgcc ataaaaataa caatacccaa
    20221 tcacagtgtc cgctggcgca tagccaaggg cttctgattg gcgcgcaagt tcaatgccgc
    20281 gcttattgtc ttctgcaata ccttcacctt gcactaaacg cctagcaagt tcatgcattg
    20341 cagcgggatc attactttta ataagccgtt caagttcttg atttgtctta tttgtataat
    20401 ttaaaatcat cataacttct tccccctttt agtataacat actaaacgcg gttgatgtta
    20461 ttgttgtttg ctttcttcta cttcgttttg taggaatggt tgtggtctaa atcttagtaa
    20521 tgtcgggaaa agcatcatta aaagaacgcc acaaataatt ccagacacac caattgcagg
    20581 taaattatat aagagtgaac cgacaaatgg tgtttcatag accactacac cactgatagt
    20641 agaaaagaaa attcgcccac ttacacccgc aggtactgca agagcaagat ataaataatt
    20701 aattatttcg tgtttatgtt tgccatacac taaagcttgg aataaagaaa ttatagaaag
    20761 aaacccatac gctaataagt aatctaaagg ataagtaatt aaaccgtatc catcccataa
    20821 catcgtgata acaccataaa ttccgcctat cccaataaag gcgtcaacaa gggggaaacg
    20881 caaagcaata atcatcagtg gtaacatcgt aatactaaat gattgtgctt caccaagccg
    20941 aaattttaaa aattctaaat caaacactaa tgctaaagca ataaatattg ccatttccgt
    21001 aataccacgc actgttaaaa tacgaccaaa aaatctgttt ggttttaatg ttcttctttt
    21061 ttttgttgtt tctttcataa ttagtctcct ttaaacaaaa agtctccacg ctgacgtgaa
    21121 gactcttatg ttacatccct acgccagcat tatctgtatc aggtgcggtc gaacgttcat
    21181 tgttcctctc agccaggcac acctagctcc cgtgagttca ttatatcaaa t
//
LOCUS       DAOSLP010000017        21072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_2766_length_21072_cov_8.852738, whole genome shotgun sequence.
ACCESSION   DAOSLP010000017 DAOSLP010000000
VERSION     DAOSLP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 21072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21072
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_21072_cov_8.852738"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..799)
                     /locus_tag="PK460_02620"
     CDS             complement(89..799)
                     /locus_tag="PK460_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE54022.1"
                     /translation="MNIITSKQNPKIKALVALKAKPKDELLIEGKHLIEMAIDANLVT
                     EIFSVEKLNYDVPMTLITPEIAEKLSGKVTTDGVFALIKKPYLKVNKNKHLLYLDEVS
                     DPGNLGTILRTALAFDFGAVLLSEGSVNAYNEKVLSAAQGAHFLLPVKKSSLDELIAL
                     KKDGYKVVATTLNDAITIEEVKASKLVLVLGNEARGVSKDVLKIADYNVKIPMQHIDS
                     LNVAVAGAILMYEISKRD"
     gene            complement(780..1445)
                     /locus_tag="PK460_02625"
     CDS             complement(780..1445)
                     /locus_tag="PK460_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOE54023.1"
                     /translation="MFNHTISVNTKNGVVNYTLILTYKRMKTIVFRPCETQENTFNVS
                     LPYGTRISKLEEVFMKNYKSLLRLQKKTSTVPFSDTTYIFGEEKSLNDIKIKYNLKEV
                     PTSLEHFYALTKKLLLSHLEESVKHYSKIMNIPVTYKVRVKRMKTRWGSNSKHTMSLS
                     FNEKLIHFHPRIINALVVHELVHYFVNGHGPKFYKLLEKYEPNYKDLDRVLKEQCYEY
                     NNE"
     gene            complement(1512..2522)
                     /locus_tag="PK460_02630"
     CDS             complement(1512..2522)
                     /locus_tag="PK460_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54024.1"
                     /translation="MKLKKLLPLSFIFLVITSCDSGIKVSKQKANKAVFAAKEKSQFH
                     NFDSVNFTYIGEVLRGKVVYNLDKTTESEVSHFSLDVKAIATDIGSDDLKMAVNYIHY
                     EPSEDDENVFEPLYKRDNYYTEEWFYTKKESIPPPTNAGVSDKLNKKLKCKMGKLPAT
                     DVLYFFNNYYGSLLIERVPRGIAEYPGFLSIVNTVEARKTKDKLVVTYTMSKEELAFF
                     LKVSFGKNIDEMTSEEIEWYKTHPWYSENIERALNGLNITSHTTTIEINKDGLISRVT
                     NDINYTVTYYETTHSVIEKTTRHYKFKNEANFVFNSKTTITFPEFIDYLDLTNVYYPE
                     NT"
     gene            complement(2619..3704)
                     /locus_tag="PK460_02635"
     CDS             complement(2619..3704)
                     /locus_tag="PK460_02635"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOE54025.1"
                     /translation="MDKKRLELLRKVSEVKGISGAERDVARLIKAELKDVADSFEYDN
                     LGSVIANLNGEEDAPRVLIAAHMDEVGFLVKQIEKGGFLRLHNVGGWWGHVIPAHEWV
                     VTTRDGKEYIGVTGAQPPHGMPAEQRNKVMELKDMYLDMGVGSDEDIKKLGIKVGDTV
                     TPLQTFRVLNDGKTLLGKAWDDRVAVAIGIEVLRRLKKEGHTANVSFAGTVQEEVGLR
                     GARTSAYKVKPDLAFALDVTMSYDLPGSPAMDTKLGAGVALSIMDGSVIGHRGLFDHV
                     ESVAKEIGVKYTYDMLTAGGTDSGEIHKVHDGVINMTLSLPCRYFHSHVSLVHSDDID
                     AAIDLLVAVIKGVDSKLIERLKATKYE"
     gene            complement(3726..4226)
                     /locus_tag="PK460_02640"
     CDS             complement(3726..4226)
                     /locus_tag="PK460_02640"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOE54026.1"
                     /translation="MEINLAPLLLKQRELDADINSRHNVTHESTKTERFLALLVEIGE
                     LANATRAFKYWSLKKSESRDVLLDEFADGLHFFLSIAIVFNFAVGKVEYESLPATKEE
                     VNNAFKRVYLRITYLMRKESKRCYTVALKEFLSLGSALEFSAQDITNAYNAKLVVNYD
                     RQQHNY"
     gene            complement(4216..6630)
                     /locus_tag="PK460_02645"
     CDS             complement(4216..6630)
                     /locus_tag="PK460_02645"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOE54027.1"
                     /translation="MFTTTKSFIHEFKKRLTEKYGVGLDDSHITERYDILGSMVRDYA
                     NINWRKSRDAVNKKGKKQLIYFSMEFLIGRLLTNNLINLGIYNVVNKGLKQIGMNLHD
                     LEEQESDAGLGNGGLGRLAACFLDSIASLGYAGNGHCIRYEYGFFRQKIVDGKQVELP
                     DQWLVNGNVWEVRKPKHSVNVKFYGHAETYQKRNGEFGIRTVNPTIVKAVPYDMSVVG
                     YRNDVANTLRLWSAEPSDEQLPRDESFDDYLRTLREINRGLYPDDSTEKGKMLRLRQQ
                     YFLVSAGLQSAIRGHFRRHKTLRNFHEHYVFQLNDTHPILAIPELMRILLDDYEYGWD
                     ESWDIATKTFAYTNHTIMVEALERWPVRYVHHLVPRNYMIIEEIHRRSQREFVNKGIS
                     EGDRQNMAIIRDGMIHMTNLAIYASFSVNGVAALHTEILKAQTFKEFYSLYPEKFNNK
                     TNGITHRRWFVASNPELNKYLTSLIGDSYITNPDDFEKLLPFVKDKKVQRKFLQIKRL
                     KKQQLAAYIKRANGIKIDVDSIFDVQIKRLHAYKRQLMNVFRIMMLYFRMKSDADFRI
                     HPVTFIFGAKAAPSYYFAKKVIELINGVADVVNNDPKISPFMKVVFIENYGVKTAEII
                     IPATDISEQISTAGKEASGTGNMKFMMNGALTLGTLDGANVEIHERVGDEHSFIFGLT
                     EPEVKALRESGTYNPWDVYHNDHEIRRILDSLIDGTWSQGNPHTFKAIFDEVMNKGDE
                     YLILKDLPAYFKRTDEIVTYYRKKSSWAESALYNIAKSGFFSSDRTIHQYIDEIWHID
                     KVKNGN"
     gene            complement(6667..8724)
                     /gene="glgB"
                     /locus_tag="PK460_02650"
     CDS             complement(6667..8724)
                     /gene="glgB"
                     /locus_tag="PK460_02650"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOE54028.1"
                     /translation="MSFIDEFLMGFTTSAHKYFGAHFNPAKGKKKKSVTFRLYAPAAS
                     DVSVIGDFNGWDPNVHKMEKVDYRGIWEITIPELENYQNYKFRFRNAHGHYVDKADPY
                     AFFSEKAPGTASRLFDYEGFIWHDEDWMKSRCRGHDKPISIYEIHLGSWKGKENGRIL
                     SYEEIADYLIPYMRDNGFTHVEIMPITQYPFDGSWGYQATGFFSVDSRYGNPMQLMSF
                     VDRMHQAGFGVILDFVAVHFAIDHYGLIEFDGTRMYEYQDSEHTFSQWGSPQFDLGRD
                     PVRSFLISAIFYFLEYFHFDGIRIDAVSNIVYWHGNSDRGVNHGGVEFLKRLNGKVHY
                     HFPGVMMIAEDSTAFDGTTRDVEWGGLGFDYKWDMGWMNDTLRYYSKDPIYKKYDHNL
                     FTFSMHYFFSENFMLPLSHDEVVHGKGTIINKMFGNYDQKFALTRNLYTYQFAHPGKK
                     LTFMGNEIASFDEWNESKSLPWNLKSYPKHDSVSRLVRDLNLIYRSEKAMHVGEFDPR
                     NFRWLMVDNRDQSVFAFQRKYGEETLIFVFNMTGNYYEYYDIGVEEEGTYTEIFNSDK
                     DVYGGYNQYNGLPVTTHAGPGPEMRPYRLTLKLGSFAAMILKKDLPAKKEEPKVEEVK
                     AKKPAAKKAPAKKAATTKKAEPKATKTATKSTATKKATKSATAKKTTTKKATTAKTKK
                     DTK"
     gene            9092..10300
                     /locus_tag="PK460_02655"
     CDS             9092..10300
                     /locus_tag="PK460_02655"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOE54029.1"
                     /translation="MSKLVLAINSGSSSLKFKLFKVPSEKVITSGLFERITQKMGAFR
                     IKTETGEVRRELPLPNHEVAVNVLTKALIEFKIVKSLDEINAVGHRVVQGGKYYNDSV
                     EFTDEVAAMVEKLSPFAPLHNPANLTGYEAFKKILPDALHVAVFDTSYHQTMPPEDYL
                     FPIPYEYYEKYDIRRYGAHGTSHKFIADEVTKINKRKPCNLIVCHLGNGASITAVKDG
                     KCVATSMGFTPLGGIMMGTRTGDLDPSVFYFVSKLEDKSPEEVYDMFNKKSGMLGVSG
                     ISSDARDVENALLQGNERAKHTFDLYSRRVADYIGQYHIRLGGTDTIVFTAGIGENVP
                     GIRKQIIDKVKDAMGIEFDDEQNSLTRGGIYGLLSTKNSKVKVYVIPTDEELMIVRDC
                     VRIAENKKKK"
     gene            10310..11299
                     /locus_tag="PK460_02660"
     CDS             10310..11299
                     /locus_tag="PK460_02660"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOE54030.1"
                     /translation="MHIYSKEIELKIKEYKKTTKAIFSLVEKYDKVAIFTHLSPDFDA
                     FGSQFGLGVYLRDHYPEKDIKIIGQSLPLYNGELYPEMDEVKDEWFNDDFLAIIVDTA
                     TLDRVSDNRYEKANHLIKIDHHPNVDPYGDICFVDVTSVAVAEMIAVMLLADGGTISK
                     DVASYLFSGIVGDSGRFKYASTTSLTFGIAEILTSLGIDLNYIYNRLYIQNLDDLRCI
                     GYILNNFKITKNGFAYYVMDEATLQELNITAERAKENVNVFADIRGIKVWAAITERTD
                     TNEWRVSIRSAGIPINEFAQKWRGGGHVQASGASLLNREEIDVFVKEMDEYIG"
     gene            11347..14334
                     /locus_tag="PK460_02665"
     CDS             11347..14334
                     /locus_tag="PK460_02665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOE54031.1"
                     /translation="MSFIPLNVFTNYALLQSALTLDDYLKTLKNRNIKAAGVSDPDTL
                     FSYPHFAKKSREFHIKPLYGVRLNFEGSSLVIYPQNEKGYRELLALLEVKNKRELTLQ
                     DLKENSTNLIVIIPSSAIHNLELKEQIMQKYFYNFAINTTSLYIGLERENAELTSFLR
                     EFSDKYNYALVAFPLIKYAKSEDHKTLLMIEAIREGKSFSIYDISSGPDYILSDAELN
                     NYYSENELKNTHLISDLINFDLSVKHDLNFPLPGAYDPNKKIDELTLNALKAKGLDNN
                     KKYIERLTHELSIIRNLGYVNYFLIVSDYVNFAKKAGILVGYGRGSAPGSLVSYLLGI
                     THADPLEHNLLFERFLNPERVSLPDIDIDFMDIRRGEVIDYIKKTYGEDKVANIVTFQ
                     TNATKASLRDTGNVFNIDPKFINRLSNSLGNTNYNFRDAYRNIKAFRDLVDNDTYNLE
                     IIKNATKIEGFPRQSGLHAAGIIIDGEPLARNLPTFFNNGLRVTQYEMGFLEEHGFLK
                     VDILGLSNLTFVHNIINLVKENHNIDIDFYNLKFDDPQIYDVIRNGHTLGLFQLESAG
                     MRKAIATIKPTNFDDVSALLALYRPGPMKYIDEYALRKEGKSPITYINDDLIDILKPT
                     YGIMIYQEQIMQIAATMAGFSLGQADILRRAIGRKDEKNILEQKDAFFAGAKKRGYSR
                     KDTEKVFDDILRFADYGFNKSHSVVYAMLTTALAYLKTYYPYEFYQEILRGLVSDNVK
                     FSLARQELKALNIKISRPCVLKSTTEFIRTNNSINFPLSAINGLNRESANIIIRLREQ
                     KAFSSVQDFFFRSFKAGVTAEEFIALIEAGALDSFNTNRAQLKVMFNTYLPTLDIGLF
                     DNEAALHAIIVPKIKESDDRLDLEISRLRFPLSGNPLDKLKLKDIKRVSHILELEDGY
                     IKTYGVLTSLRLIKTKKGDEMAFATASDYEEDINLVFFPRVLAEVKGMLTRNNIYIIA
                     GKLETRDGQKQLVVNTLERVQNE"
     gene            14327..16882
                     /gene="polA"
                     /locus_tag="PK460_02670"
     CDS             14327..16882
                     /gene="polA"
                     /locus_tag="PK460_02670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOE54032.1"
                     /translation="MSKRLIIIDGNSLLFRAYYATAYMGTDKIMRTSTGVPTNAIFAF
                     ANMIVRIVNDLKADDHLFVAFDTGEKTFRHEEYDDYKANRQAAPDELVTQFPIAREFL
                     DALSIRHDEVVGYEADDLAGSLAKAAGKEGFKVELYTSDQDYLQLIDEDISVQLIRRG
                     LSDVRFLDPKGLYEEFGYHAYQVPDFKGLTGDASDNLKGVPGVGKVTATKLLAEYDTL
                     EKILENADNIKGKLGENIREHKATALQSKHLATILTDLPLPCPLNDFHYKGFDNSAVH
                     AFAQKYEMRQFLNNLKYTNSEVEEEIKAHEIKSFNEVKEKDYSYISFYVTASDEDYYK
                     ATPEFIYFSLGKNVYRISFNDLLLDPKLKALFSDEALLKYTYDYKRHAIMLSNHALDI
                     KGTVNDALVLTYVWDTNVAPNISAFLLTYGVNASSEEEETYAILVAKLSAIYQEGINK
                     LKKENLLQVYEDIEKPLTDVLIKMEKAGFNVDKDVLTAIGSEYKVILDNLTRTIFLLA
                     GHEFNINSPKQLATILFDELGLKANRKRSTSADVLNDLKDKHPIIPAILEYRKYQKII
                     STYIDGFIPHIHRDGKVHTTFNQALTTTGRLSSNNPNLQNISIRHEEGREIRKAFNAG
                     DKDVLAIDYSQIELRLLAAIANVPSLIDIFNTDRDIHSETAIALFPLEDLNYARRKAK
                     AVNFGIVYGISDFGLSEQLETTVKDARAIIQSFFKTYPEVWTYRQEVINELIKDGYVK
                     TLTGRKRYIPQINDPNYQTREFAKRAAMNAPLQGSAADLIKMAMIKVDEFLTKNKCKT
                     KLILQVHDELIFALDQDEHEIVDDLVNIMENVLDLPVKLKVEVKTGKTWFEAS"
     gene            16888..18261
                     /gene="mutM"
                     /locus_tag="PK460_02675"
     CDS             16888..18261
                     /gene="mutM"
                     /locus_tag="PK460_02675"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOE54033.1"
                     /translation="MPELPEVETVKKILNKVIVGAKITDVIIHKENIITGDPHTFKKT
                     LIGETFSEVKRIGKYLIFTFESDLIMLSHLRMEGKYIEVLPGTEDSRYARVIFVLNDG
                     RRLCYDDSRQFGTLELSTKDTYLQTKSMQNVGREPFDIDPIDYFNKISNRTTPIKQTL
                     LDQKLMSGLGNIYVDEVLYLSKIHPETPTNMLGLMAINLLIENSIKVLNAAIKKGGTT
                     VRSYSPAEGVHGNFQQELNAYGKAGERCPVCNFKFKKIFVGGRGTTYCPSCQKNPAVP
                     LTVAITGQKASGKTLIANYLRKNGHKVIDTDKLAKELYKDIKITTDLEKHLGVNLHDN
                     TTFNLQYLRNYLVENENEIKKLNDFIHPLVKKEIIEIIKTSNEAILYFEVPLLFSHKI
                     YELFNFMIGVEVSVEKQLKNLEKRGDTHTINADEAYMKNRHKLDLIIVNDGSEQDLIE
                     KFKAFKV"
     gene            complement(18251..19129)
                     /locus_tag="PK460_02680"
     CDS             complement(18251..19129)
                     /locus_tag="PK460_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54034.1"
                     /translation="MKKVELDIDTTNVEEIRQEMYDKIMSHKSFVKLLEEKGATPEII
                     KNNVGLLNDYLESYLSVEKCRKAGHCVSDEKHHALTIQIDAPFVTVERHLCPVYLESQ
                     SLAMRFIVRDFPENYLDLRVEDLPARRGFAAFIRELMKVLKGDIYFIYAYGTAGIGKL
                     KTTIPFVTKMLEDDKNKTAGVVNFPAFVRASIADYYDKKDIVDSYLQHYIDLDVLVLD
                     NFGNEEVNKIVIESIIFPLLAARVRQKKPTIILAGLSTNDLRSLYVGRSVRGHQIVDL
                     IKANIKEEIYLQGTKL"
     gene            complement(19126..20373)
                     /locus_tag="PK460_02685"
     CDS             complement(19126..20373)
                     /locus_tag="PK460_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54035.1"
                     /translation="MRLIKSNDFYSVKVRGYVTSLHLEVLDILYQPIMGFAATALYRY
                     LFTMRDFRKGDPLSFTLIHDHFGFSYDQISIAFSKLEGLGLMRSFYVEYNEFSEYVLE
                     LYAPKDPAAFSQDQIFMNLLNDVLGPRHTQDLLSLFTLDRETLNKEEVTSTFAEIYAD
                     RIFDIKGDDLYKGEPLENVVGNIKFEFDRAAFVNILTRERKILPSALSTEELKKAEQM
                     AGLYNLTEVATAERVGDFYDALQPFGARVDFVAMNKHLQELVKYPVLSKPLRRPVQEL
                     AGESQKIKRINLMETYSPVEFLQYLNEGTKIAPADYNILTTLSHDYNLLSPVINALVY
                     YTLYHNNNELIRALVEKNASLLARNKVTTALDALDTLERPIKKRGRAPKKAPILNDDK
                     KEEATPQVDDLDDPIWDELKKLK"
     gene            20574..>21072
                     /locus_tag="PK460_02690"
     CDS             20574..>21072
                     /locus_tag="PK460_02690"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOE54036.1"
                     /translation="MTVKVAVLTSGGDAPGMNAAIRAVIRSGLLAGYEMFAIFEGFRG
                     IIENKIVSVDRTFVSEIIMRGGTILKTARFPEFRDSKVVEKAAENLRFYGITHLIVIG
                     GDGSYRGAYALSKHGIKIIGIPATIDNDVMGTEQTIGFDTALNTITEAVDKLRDTSSS
                     HHRCSI"
BASE COUNT         6723 a         3863 c         3694 g         6792 t
ORIGIN      
        1 caaataatct attttaaata taaaaattgt aagaaaatgt taaaaaaagg aagttgatgc
       61 aacaaaacaa taaaattatt gctacttgtt agtctctttt acttatttca tacatcagta
      121 ttgcccccgc aacagcaaca ttaagcgaat caatgtgttg cattggaatt tttacattgt
      181 aatcagcgat ttttagaaca tctttactca caccacgcgc ttcattccct aggacgagca
      241 ctaatttaga agccttaact tcttcaatag taatagcgtc gttaagcgtt gtagcaacaa
      301 ctttataccc gtctttttta agtgctatta attcatcaag tgaagacttt ttaacgggga
      361 gtaaaaagtg tgcaccttgt gcagcgctta atactttttc gttatacgcg ttaacactac
      421 cttcactaag caaaactgcg ccaaagtcaa aggctaaagc ggtgcgtaaa atagtaccta
      481 agttaccagg atcacttact tcatctaaat agagtaaatg tttattttta ttaactttta
      541 gatatggttt ttttataaga gcaaacacgc catcagtggt tactttacca cttaattttt
      601 ccgctatttc aggagtaata agcgtcatag gaacatcgta atttagtttt tcaacactaa
      661 agatttcagt gactaagttt gcatctatag ccatttcaat aaggtgttta ccttcaatta
      721 aaagttcgtc ttttggttta gctttaagag ccactagtgc tttaatttta gggttttgtt
      781 tactcgttat tatattcata acattgctcc tttaacactc tatctaaatc tttataattt
      841 ggttcatact tttctaacag cttataaaac ttagggccat gcccattaac gaaataatgc
      901 actaattcgt ggacgactaa agcattaata atacgcggat gaaagtgaat taatttttca
      961 ttaaaactta aactcattgt atgtttacta ttactacccc accttgtttt catccgttta
     1021 actcttactt tgtaagtgac aggtatattc ataattttag aatagtgttt cacactttct
     1081 tctagatgac ttaaaagtaa cttttttgtt aaagcataga agtgttcaag acttgtcggt
     1141 acttctttta agttatattt tatttttatg tcattaagac tcttttcttc gccaaagatg
     1201 taagtcgtat cactaaaagg gacagtgctt gtcttttttt gtagtcttaa aagactctta
     1261 taatttttca taaacacttc ttctaatttt gagattcgtg tgccataggg aagcgaaaca
     1321 ttaaatgtat tttcttgcgt ttcacacggt cgaaaaacaa tcgtcttcat tcttttatag
     1381 gtaagaatta aagtatagtt aacgacacca tttttagtat taacactgat agtatgatta
     1441 aacataaatc tattgtatca tgagagagta attaatattt ttgaagaata aataatgaca
     1501 gcacttttta tttacgtgtt ttcggggtaa tacacattag taagatcgag atagtcaata
     1561 aattcgggaa atgttatagt ggttttacta ttaaatacaa aatttgcttc attcttgaat
     1621 ttataatgtc ttgttgtttt ttcgattacc gaatgcgttg tttcgtaata tgtaactgta
     1681 taattaatgt cattagtgac tctactaata agaccatcct tattaatttc aatagtcgtt
     1741 gtatggctag taatatttaa accgtttagt gctcgttcaa tgttttctga ataccacgga
     1801 tgagttttat accattcaat ttcttcactt gtcatttcat caatgttttt accaaaactt
     1861 acctttaaga aaaacgctag ttcttctttt gacattgtat aagtcaccac tagtttatct
     1921 ttagttttac gcgcctcaac agtatttact attgataaaa agccagggta ttccgcaatt
     1981 ccgcgcggaa cacgttctat taataatgaa ccataataat tgttaaaaaa atataaaaca
     2041 tccgtcgcgg gaagttttcc cattttacat ttgagttttt tatttaactt atcacttaca
     2101 ccagcatttg ttggtggtgg tattgattct ttcttggtgt agaaccattc ttccgtgtag
     2161 taattgtcac gcttgtaaag tggctcaaaa acattttcat catcttcact aggttcataa
     2221 tgaatataat ttacggccat ttttaggtca tcgcttccta tatcggtcgc aattgctttt
     2281 acatctagtg aaaaatgact tacttcactt tcagtcgttt tatcaaggtt ataaactact
     2341 tttcctctta atacttcccc aatatatgta aaatttacag aatcaaaatt atggaattgt
     2401 gacttttctt tcgccgcaaa taccgcttta ttagcttttt gtttagatac tttaatgccg
     2461 ctatcgcagc tagtaataac taaaaagata aaagataaag gcaataattt cttaagtttc
     2521 ataactcata ctccataatt tattgtacca ctatttattc tataaataaa aagtaccgaa
     2581 gttttaccct cggtactctt acactttaaa ttatttaact attcgtattt agtcgctttt
     2641 aagcgttcaa ttaacttact atcaacgcct ttaatgaccg cgacgagtaa atcaatagct
     2701 gcatcaatgt catcactatg cacaagtgag acatgactat ggaaatagcg gcacggtaga
     2761 gatagcgtca tattaatgac accgtcatgc accttatgaa tttccccact atcagtgcca
     2821 ccagcagtta acatatcgta agtatattta acgccaattt cttttgcgac actttcaaca
     2881 tggtcaaaaa gtccgcgatg gccaatcacg ctgccgtcca taatgctaag cgctactcct
     2941 gctcctagtt ttgtgtccat tgctggtgac ccaggtaagt cgtaactcat tgttacgtca
     3001 agggcaaaag ctaggtcagg tttaactttg tatgcactag tgcgtgctcc gcggagtccc
     3061 acttcttctt ggacagtacc ggcaaaacta acatttgcgg tatggccttc ttttttcagg
     3121 cgacgtaaaa cttcaatccc aattgcaact gcgacacggt cgtcccacgc tttccctaaa
     3181 agtgttttac catcatttaa tacccggaaa gtttgtaagg gtgtaacagt gtcaccaact
     3241 ttaattccaa gttttttaat gtcttcgtca ctaccgacac ccatatcaag atacatatct
     3301 tttagttcca tcactttatt gcgttgttca gcgggcatgc cgtgcggagg ttgagcacca
     3361 gtgacaccaa tatattcttt accatcacgc gttgtcacga cccattcgtg ggctgggata
     3421 acgtgacccc accagccacc aacattatgg agccgtaaaa agccaccttt ttcaatttgt
     3481 ttaacaagga agccaacttc atccatatgc gcagcgatta agacacgggg tgcgtcttct
     3541 tctccattta aatttgcgat aactgaacct aggttatcgt attcaaaaga atctgcaaca
     3601 tcttttaatt ctgctttaat aagacgagcg acatcgcgtt cagcaccgct gatgcctttg
     3661 acttcactta cttttcttaa taattctaaa cgttttttat ccatcatgac ctctttctat
     3721 tacttctagt aattatgttg ttgtctgtca tagtttacca ctagtttagc gttataggca
     3781 ttagttatgt cttgggcact aaactctaaa gcgctaccaa gacttaaaaa ttcttttaaa
     3841 gcgactgtgt aacagcgttt actttctttc cgcattaagt acgtaatccg taaataaaca
     3901 cgcttaaagg cattatttac ttcctcttta gtcgccggta aactttcgta ctctacttta
     3961 ccgactgcaa aattaaagac aatcgcaatt gagaggaaaa aatgtaaacc gtccgcgaat
     4021 tcatcaagta gtacatcgcg actttcactt tttttaagcg accaatattt aaaggcacgc
     4081 gttgcattag cgagttcacc tatttccaca aggagcgcta aaaagcgctc cgttttagta
     4141 ctttcgtgag tgacattatg acgactattt atatcagcgt caagttcacg ttgtttaagg
     4201 agtaatggtg caagattaat ttccattttt taccttgtca atatgccaaa tttcatcgat
     4261 atattgatga attgtgcggt cactgctaaa gaacccgctc ttagcaatat tatagagcgc
     4321 actttccgcc cagcttgatt tcttccgata atacgtgaca atttcgtctg tgcgtttaaa
     4381 gtaggctggt aaatctttta agattaagta ctcgtcaccc ttattcatta cttcgtcaaa
     4441 tattgctttg aaagtatggg ggttaccttg actccatgta ccgtcgatta atgaatctaa
     4501 tatccgacga atttcatggt cattatggta aacatcccac ggattataag tacctgattc
     4561 tctaagtgct ttcacttctg gctcagttaa gccaaagata aaggaatgtt cgtcccctac
     4621 gcgctcatga atttccacat tcgcgccgtc tagtgtccct aaagttaagg caccattcat
     4681 cataaacttc atattaccag tgccgcttgc ttctttccca gcggtactaa tttgttcact
     4741 aatatcggtc gcaggaataa taatttcggc agtcttgacg ccatagtttt caataaagac
     4801 aactttcata aatggcgaaa tttttggatc attgttaacg acatcagcga caccgttaat
     4861 taattcaatt actttcttcg caaagtagta acttggtgcg gctttcgcgc caaagataaa
     4921 ggtgacagga tgaattctaa aatcagcatc actcttcatc cggaaataaa gcatcataat
     4981 gcgaaataca ttcattaatt gccgtttata agcgtgtaaa cgtttaattt gcacatcaaa
     5041 aatcgaatca acatcaattt taatgccgtt agcgcgctta atataggcgg caagttgttg
     5101 ctttttgagg cgtttaattt gtaagaattt ccgttgcact ttcttatctt ttacaaaagg
     5161 taagagtttt tcaaagtcat cgggattagt aatataagag tccccaataa gacttgttaa
     5221 atatttatta agttccgggt tacttgccac aaaccagcga cgatgcgtaa tcccgtttgt
     5281 tttattatta aatttctcag gataaagact gtagaattct ttaaatgttt gcgcttttaa
     5341 gatttcagtg tgtagcgctg cgacaccgtt aacactaaaa ctagcgtaaa tcgctaagtt
     5401 tgtcatatga atcatcccgt cgcggataat tgccatattt tggcgatcac cttcactgat
     5461 gcctttatta acgaattcgc gttgactacg acgatgaatt tcttcaataa tcatgtagtt
     5521 gcgtggcact aaatgatgga cataacgcac tggccagcgt tcaagtgctt cgaccatgat
     5581 tgtatggttt gtataggcaa aagtcttagt agcgatatcc cacgactcat cccagccata
     5641 ttcataatca tcaagtaaaa tacgcattaa ttccggaatt gctaaaatgg ggtgcgtatc
     5701 attaagttgg aacacatagt gttcatggaa attacgtaac gttttatgac gacggaaatg
     5761 accgcgaatc gcactttgca agccagcact gactaagaaa tattgctgtc ttaagcggag
     5821 cattttacct ttttcggtag aatcatcagg atataacccg cgattaattt cgcggagtgt
     5881 ccgtaaataa tcatcaaatg attcgtcacg tggtaattgt tcatcacttg gttcagcgct
     5941 ccataagcgg agagtatttg cgacgtcatt acgatagccg acaacactca tgtcataggg
     6001 cactgcttta acgatagtgg gattaacagt tctaatccca aattcaccat tgcgtttttg
     6061 ataagtttca gcgtgaccgt aaaacttaac attcaccgag tgctttggtt tccgtacttc
     6121 ccagacgttg ccattgacta accattgatc gggtaattcg acttgtttcc cgtcgacaat
     6181 cttttgccgg aagaacccgt attcataacg aatacagtga ccgtttccag cgtaaccaag
     6241 actcgcaatt gaatcaagga aacatgcggc aaggcgacct aaaccaccat taccaaggcc
     6301 agcatcactt tcttgttctt ctaaatcatg aagattcatc ccaatttgtt ttaggccttt
     6361 gttaacgaca ttataaattc ctaagttgat taggttgttt gtcagtaagc gaccaattaa
     6421 aaattccatt gagaaataaa ttaattgttt cttacctttc ttattaacag cgtcacggct
     6481 tttgcgccag ttgatgttcg cgtagtctct taccattgaa cctaaaatgt cataacgttc
     6541 agtgatgtga ctatcatcaa ggccaacgcc gtatttctct gttaatcgct ttttaaattc
     6601 atgaataaaa ctttttgttg ttgtaaacat aaattattcc ttcctttatt tatttaactc
     6661 actaaattat ttagtgtctt tttttgtttt agcagtagtt gcttttttcg ttgtagtctt
     6721 tttggctgta gcactttttg ttgctttttt agtagcggtg ctctttgtgg cagtttttgt
     6781 tgccttaggt tcagcctttt ttgttgtcgc tgctttttta gcaggcgctt ttttcgctgc
     6841 tggtttcttt gctttaactt cttcgacctt tggttcttct ttctttgcgg gtaaatcttt
     6901 ctttaaaatc atcgcggcaa atgaaccaag tttaagtgtt aagcggtatg gacgcatttc
     6961 tggtcctgga ccagcgtgtg ttgtcactgg taaaccgtta tattggttat aaccaccata
     7021 gacatcttta tcactattaa agatttcagt gtaagtacct tcttcttcaa caccgatatc
     7081 gtaatattcg taataatttc cagtcatatt aaagacaaag attaatgttt cttcaccgta
     7141 tttacgttgg aacgcaaaca cactttgatc acgattatcg accattaacc atctaaagtt
     7201 acgagggtca aattcaccaa catgcatcgc tttttcacta cgataaatta agtttaagtc
     7261 gcgcactaag cgactaactg aatcgtgttt tggatatgac tttaagttcc acggtaaact
     7321 cttactttca ttccactcat cgaagctagc aatttcgtta cccataaagg taagtttttt
     7381 accaggatgc gcgaattgat aagtatataa gttacgcgtt aaggcaaact tttggtcata
     7441 gttaccaaac atcttattaa taattgtccc tttaccgtgc actacttcgt cgtgacttag
     7501 tggtaacata aagttttcac taaagaaata atgcatcgag aatgtaaaga ggttatggtc
     7561 atatttctta taaattgggt ctttactata atagcgtaag gtgtcattca tccaccccat
     7621 atcccattta tagtcaaagc ctaaaccacc ccactcaacg tcccgcgttg taccgtcaaa
     7681 cgctgttgag tcttcagcaa tcatcataac accagggaaa tggtaatgaa ctttaccgtt
     7741 gagtcgtttt aagaattcaa caccgccgtg atttactccg cggtcactat taccgtgcca
     7801 gtagacaatg ttactgacag cgtcaattct aattccgtcg aaatggaagt attctaagaa
     7861 ataaaaaatc gcactaatta agaagctacg aactggatcg cgccctaaat caaattgcgg
     7921 ggaaccccat tgactaaatg tatgctcact gtcttgatat tcatacattc ttgtcccatc
     7981 aaattcaatt aaaccataat ggtcaatggc aaagtgaaca gcaacaaagt ctaaaataac
     8041 gccaaaaccg gcttggtgca tacggtcgac aaatgacata agttgcattg ggttaccata
     8101 acgactatca acactaaaga aaccagtagc ctgataaccc catgacccgt caaatggata
     8161 ttgtgtaatc ggcatgattt cgacatgagt aaagccatta tcacgcatat aaggaattaa
     8221 gtaatcagcg atttcttcgt aagataaaat ccgcccattt tcttttcctt tccagctgcc
     8281 taaatggatc tcgtaaatac taattggttt atcatgacct ctacatctac ttttcatcca
     8341 atcttcgtcg tgccaaataa aaccttcata atcaaatagc cgtgaagcag tacctggtgc
     8401 tttttcacta aagaaagcat atgggtcagc tttatctaca taatgaccat gggcattccg
     8461 gaatcggaat ttatagtttt ggtaattttc taattcagga atagtaattt cccaaatacc
     8521 acggtaatcg accttctcca tcttgtggac atttggatcc caaccgttaa aatccccaat
     8581 cacacttaca tcacttgccg ctggggcata gagacgaaat gttacacttt ttttcttttt
     8641 acctttcgcg ggattaaaat gagcgccaaa atatttatgt gcactcgttg taaaccccat
     8701 caggaattca tctataaaac tcataatcaa cctccctcgt tttattatta taataaaaaa
     8761 ggacgaagaa tggtagttta aacaccggaa ttatcgcaat tttgacagaa aatgaaaatt
     8821 taccgacaaa tttagacgaa taatattcaa tttattgaaa ttttatgcat tatcggtatt
     8881 ttttaaatat ttgagttatt acaattttag agttgttact agcttattgc caaattattt
     8941 atcgcaaaat attaatccgc aaacaagata aaaagataat ttgctcttat tattaataat
     9001 ataggggaaa taattaatca acttttttgt aacaaaatca cagcacgcta acttgtcttt
     9061 gtgtataata ataaaatgag aggtgaaata tatgtcaaaa ttagtgttag ctattaactc
     9121 gggaagcagt tccttaaaat tcaaactttt caaagttcca agtgaaaaag taattacaag
     9181 tggacttttt gaaagaatta cacaaaaaat gggagcgttt cgcattaaaa ccgaaacggg
     9241 tgaagtaaga cgcgaactac cactaccaaa tcacgaagta gcggttaatg ttttaacgaa
     9301 ggctttaatt gaatttaaaa tcgttaaatc attagatgaa attaacgcag tgggtcaccg
     9361 tgttgttcaa ggcggtaaat attacaatga tagcgttgaa tttaccgatg aagtcgcggc
     9421 aatggtggag aaacttagtc cttttgcccc acttcataac cccgcaaact taacgggcta
     9481 cgaagctttt aagaaaatct tgccagatgc actgcatgtt gctgtctttg atacttctta
     9541 ccatcaaaca atgccaccag aagattattt attccccatt ccttatgaat attatgaaaa
     9601 gtatgacatc cgtcgctacg gagcacacgg tacttcacat aaatttattg ctgatgaagt
     9661 tacaaaaatt aataaaagga aaccgtgtaa tcttatcgtc tgtcacttag ggaacggcgc
     9721 aagtattact gctgttaaag acggaaaatg tgttgctaca tcaatgggct ttactccttt
     9781 aggaggcatc atgatgggta cacgaactgg tgaccttgat ccaagcgtct tttattttgt
     9841 tagtaaatta gaagataaat cacccgaaga agtttatgac atgtttaata aaaagtcagg
     9901 aatgcttggt gtcagcggta tttctagtga tgcacgcgat gttgaaaatg ctcttcttca
     9961 aggcaatgaa cgcgctaaac acacctttga tttatatagt cggcgtgtcg ctgattatat
    10021 tggtcaatat catattcgtt taggtggtac tgacaccatt gtctttacgg ctggtatcgg
    10081 tgaaaatgtc ccaggcattc gtaaacaaat tatcgacaaa gttaaagatg cgatgggcat
    10141 tgaatttgat gacgaacaaa atagcttaac tagaggtgga atttatggtt tactttccac
    10201 gaaaaatagt aaagtaaaag tatatgtgat tccgactgat gaagaattaa tgattgttcg
    10261 agattgtgtg cgaatcgctg aaaataaaaa gaaaaaatag gtgtaattaa tgcacatata
    10321 ttcaaaagaa attgaactta aaatcaaaga gtataaaaaa acaaccaaag caatttttag
    10381 tcttgttgaa aagtatgata aggtagctat tttcacgcac ctttcaccag actttgacgc
    10441 cttcggttct caattcggtt taggagtata tttacgcgac cattatcccg agaaagacat
    10501 caaaattatt gggcaaagtt taccacttta taacggtgaa ctttatcccg aaatggatga
    10561 agtaaaggat gaatggttta atgatgattt tttagcaatt attgttgata ccgcgacgct
    10621 tgaccgtgtt agtgataatc gttatgaaaa agctaaccat ctcattaaaa ttgaccatca
    10681 tccaaatgtt gatccctacg gtgatatatg ttttgttgat gttactagtg ttgccgtcgc
    10741 cgaaatgatt gccgttatgc ttttagcaga tggcggcact atatcaaaag atgtcgctag
    10801 ttatctattt agtggtatcg ttggtgattc tggacgcttt aaatatgcgt ccactacttc
    10861 acttaccttc ggtattgccg aaatcttaac aagtttaggc attgatttaa attatatata
    10921 caatcgttta tacatccaaa acttagatga cttacgctgt attggttata tcttaaataa
    10981 ttttaaaatt acgaaaaatg gttttgccta ttacgtgatg gatgaggcta cactgcaaga
    11041 actcaatatt actgctgaac gcgcaaaaga aaatgttaat gttttcgctg atattcgcgg
    11101 gattaaagtc tgggccgcca ttaccgaacg cactgacaca aacgagtggc gggtatcaat
    11161 acgcagtgct ggtataccga ttaatgaatt cgcccaaaaa tggcgaggcg gtggccatgt
    11221 gcaagctagt ggtgcttcac ttttaaaccg tgaagaaatt gatgtatttg tgaaagaaat
    11281 ggatgaatat ataggctaat aagcctattt tcatttcttg tctcttcaat atgttaaaat
    11341 aattatatgt cttttatccc tttaaatgta tttacaaact atgcgctcct tcaaagtgca
    11401 cttactttgg acgattattt aaagacactt aaaaatcgaa acatcaaagc agcgggtgtt
    11461 agtgatcctg ataccctttt tagttatccg cactttgcca aaaaaagtag ggaatttcat
    11521 ataaaaccac tctatggtgt taggcttaat tttgaaggtt cttcactagt tatttatcct
    11581 caaaacgaaa agggatatcg tgaactttta gcgctccttg aagtcaaaaa taaacgcgaa
    11641 ttgacgctgc aagatttaaa agaaaatagc acaaatttaa ttgttattat tccctcttcc
    11701 gctattcata atttagagtt aaaagagcaa attatgcaaa aatattttta taattttgcc
    11761 attaatacaa catcattata catcggttta gaacgggaaa atgcagaatt aacatccttt
    11821 ttgcgagaat ttagtgataa atataactat gcactagtag cgttcccgct tattaaatat
    11881 gctaaaagtg aagaccataa aacactttta atgattgagg caattagaga aggtaaatcg
    11941 ttttccattt atgatatttc tagtggtccc gattatattt taagtgacgc tgaacttaat
    12001 aattattata gtgaaaatga acttaaaaat acgcatctaa ttagtgattt aattaacttt
    12061 gatttaagcg ttaaacacga cttaaatttc ccacttcctg gcgcatatga tcctaataag
    12121 aaaattgatg aattgacact aaatgcgctt aaagcaaaag gcttagataa taataaaaaa
    12181 tacatagaac gactcacaca tgaattaagt atcattcgta atttaggcta tgttaactac
    12241 ttcttaatcg ttagtgatta tgttaatttt gcaaaaaaag ctggaatctt agtcggttac
    12301 ggtaggggta gtgcaccggg ttcacttgtt agttatcttt taggtatcac ccacgctgat
    12361 ccacttgaac acaatttact ttttgaacgc tttttaaatc ctgaacgtgt ctctttgcca
    12421 gacattgata tcgactttat ggatattaga cgcggtgaag taattgatta tattaaaaag
    12481 acttatggtg aagataaagt cgctaatatt gtgactttcc aaactaatgc cacaaaagca
    12541 tcgttgcgtg atacaggtaa tgtttttaac attgatccca aatttattaa tcgcttaagt
    12601 aactctttag gtaacactaa ctataacttc cgtgatgctt atcgtaacat taaagcattc
    12661 cgtgatttag tggacaacga tacttataac ttagaaatta ttaaaaatgc tactaaaatt
    12721 gaaggatttc cgcggcaaag tgggcttcac gctgctggta ttataattga cggcgaaccg
    12781 ctcgcgagaa acttaccaac attttttaat aacgggttac gcgttacaca atatgaaatg
    12841 ggattccttg aagaacacgg atttttaaaa gttgatattt taggactatc aaaccttacc
    12901 tttgttcata acatcattaa tttagtgaaa gaaaatcata atatcgacat tgatttttat
    12961 aatttgaaat ttgatgatcc gcagatttat gatgtaatcc gtaatggtca tacattagga
    13021 cttttccaac ttgaaagcgc cggaatgcgg aaagcaattg cgacaattaa accgactaat
    13081 tttgatgatg ttagcgcctt acttgcgctt tatcgtcctg gtccgatgaa atatattgat
    13141 gaatacgctc tgcgtaagga aggaaagtca ccgataactt acatcaatga cgacttaatt
    13201 gatatcctaa aaccaacata cggcattatg atttatcaag aacaaattat gcaaattgcc
    13261 gcgacaatgg ctggctttag ccttgggcaa gccgatatct tacgacgggc gattgggcgt
    13321 aaagatgaaa aaaatatcct agagcaaaaa gatgcatttt tcgctggtgc taaaaagcga
    13381 ggctactcaa gaaaagatac cgaaaaagtg tttgatgata ttttacgctt tgctgattac
    13441 ggctttaata aatcgcactc tgtagtctac gcgatgctca ctactgcttt agcgtatctt
    13501 aaaacttatt atccttatga attctatcaa gaaatattac gcggtttagt aagtgataat
    13561 gttaaattct ccttagcgag acaagaatta aaagcactta acatcaagat aagtaggcct
    13621 tgtgtcttaa aatctacaac tgaatttatt cgcactaata actcgattaa tttcccactt
    13681 agtgcgataa acggattaaa cagggaatcc gcgaatatca ttattagatt acgtgaacaa
    13741 aaagctttta gtagtgtgca agacttcttc ttccgcagtt ttaaggctgg tgttaccgct
    13801 gaagaattta tcgcccttat tgaagctggg gcacttgact cttttaacac taatcgcgca
    13861 cagttaaaag taatgtttaa cacctactta ccaactttag acattggctt atttgataat
    13921 gaagcggctt tacatgcgat tattgttcct aagataaaag agagtgacga ccgtcttgat
    13981 ttagaaataa gtaggctgcg cttcccgctt tcaggtaatc cgcttgataa acttaaacta
    14041 aaagacatta aacgcgttag tcatatttta gaattagaag acggttacat taaaacttat
    14101 ggtgtactaa catcattacg gcttattaag acgaaaaagg gtgacgagat ggcttttgcg
    14161 accgctagtg attatgaaga agacattaat cttgtcttct ttccgcgtgt acttgcggaa
    14221 gttaaaggga tgttaacccg caataatatt tatattatcg ctggtaaact tgaaaccagg
    14281 gacggccaaa agcaacttgt agttaacact ttagaaaggg tccaaaatga gtaaacgact
    14341 tattattata gatggaaatt ctttgctttt tagagcatat tacgctactg catatatggg
    14401 cacagataaa attatgcgca caagcactgg cgtaccgaca aacgccattt ttgcttttgc
    14461 gaatatgatt gtgcgcattg ttaatgattt aaaggctgac gatcatttat ttgttgcctt
    14521 cgataccggc gaaaaaacat ttagacatga agaatatgat gattataagg ctaatcgcca
    14581 agcagcaccc gatgaattag taactcaatt tccgattgct cgtgaatttc ttgatgcgct
    14641 ttcgattcgt cacgacgaag ttgtgggata cgaagctgat gacttagcag gaagtcttgc
    14701 taaagcagca ggtaaagaag gttttaaagt cgaattatac actagtgatc aagattattt
    14761 acaattaatt gatgaagata tcagcgtgca gcttattcgc cgcggactaa gtgacgtgcg
    14821 ctttttagac ccaaaaggac tatatgaaga atttggttat cacgcttatc aagtaccaga
    14881 ctttaaaggg ttaacggggg acgcaagtga taacttaaaa ggagtgccag gcgttggtaa
    14941 agttaccgca acaaaattac ttgccgaata tgacacttta gaaaaaatac ttgaaaatgc
    15001 cgacaatatt aaaggtaaac ttggggaaaa cattcgagaa cataaagcta ctgccttaca
    15061 gtcaaaacac ttagcgacga ttctcactga cttaccgcta ccgtgtccgc ttaatgactt
    15121 ccattataaa ggctttgaca atagtgctgt tcacgccttt gcgcaaaagt atgaaatgcg
    15181 ccaattttta aataacttaa aatatacaaa tagtgaagtt gaagaagaaa ttaaagctca
    15241 tgaaatcaaa agttttaatg aagttaaaga aaaagattat agttatattt ccttttatgt
    15301 taccgctagt gatgaagatt attacaaggc gacaccagaa tttatttatt tctcacttgg
    15361 gaaaaatgta tatcgaataa gctttaacga tttacttctt gatccaaagc ttaaagcttt
    15421 atttagtgat gaagcacttc ttaaatatac ttatgactat aaacgtcatg ccattatgct
    15481 aagtaaccac gctctagata taaagggcac agttaatgac gcacttgtct taacttatgt
    15541 atgggacacg aatgtggcac ctaacatcag cgctttctta ttaacttacg gggttaacgc
    15601 tagtagtgag gaagaggaaa cttacgctat tttagttgcg aaattaagtg ctatttatca
    15661 agaaggcatt aataaactta aaaaagaaaa tttgctccaa gtttatgaag acattgaaaa
    15721 accactgact gatgtattaa taaagatgga aaaagccggg tttaatgttg ataaagatgt
    15781 gttaaccgca attggaagtg aatataaagt cattcttgat aacttaacgc gtacaatttt
    15841 cttgctcgca ggtcatgaat ttaatattaa ttccccaaaa caactagcga caatcctttt
    15901 tgatgaattg gggctcaagg ctaatcgtaa acgctccact tccgctgatg tgttaaatga
    15961 cttaaaagat aagcatccaa ttattcctgc tattcttgaa taccgcaaat atcaaaagat
    16021 tatttcaact tatattgatg gctttattcc ccatattcat cgtgacggga aagttcatac
    16081 tacctttaac caagccttaa cgacaacggg tcgactaagc agtaataatc caaacttaca
    16141 aaatattagc attcgccatg aagagggacg cgagataaga aaagcattta atgccggaga
    16201 taaggatgtt ttagcaattg actatagtca aattgagtta cgcttacttg ccgctatcgc
    16261 taatgttcct agtcttattg atatttttaa cactgaccgc gatattcata gtgaaaccgc
    16321 aatagcacta tttccgcttg aagacttaaa ttacgcaaga cggaaagcaa aagccgttaa
    16381 ctttggcata gtgtatggca ttagtgattt tggcttaagc gaacaacttg aaacaacagt
    16441 aaaagacgcc cgcgctatca ttcagtcctt cttcaagact tatcccgagg tatggactta
    16501 tagacaagaa gtaattaatg agttaattaa ggatggttac gttaaaacat taactggtcg
    16561 taaacgctat atcccgcaaa ttaatgatcc taactatcaa acacgcgaat ttgcaaagcg
    16621 tgccgcgatg aatgctccgc ttcaaggcag cgctgccgat ttaattaaaa tggcaatgat
    16681 taaagtcgac gaatttctaa caaagaataa gtgtaaaaca aaactaattt tacaagtcca
    16741 tgacgaactt atctttgcgc ttgatcaaga tgaacatgaa attgtcgacg atttagtgaa
    16801 tattatggaa aatgtccttg atttaccagt aaaattaaaa gtggaagtta aaaccggaaa
    16861 aacatggttt gaagcgagtt aataattatg ccagaactac cagaagttga aacagttaaa
    16921 aaaatcttaa ataaagtcat tgttggggca aaaattactg atgttattat tcataaagaa
    16981 aacatcatca ctggggaccc acacactttt aaaaagacac ttattggtga aacttttagt
    17041 gaagtaaaac gcattggtaa atacttaatc tttacctttg aaagtgattt aattatgctt
    17101 agtcacttac ggatggaagg taagtatatt gaagttttac cgggtactga agattcgcgt
    17161 tacgctcgtg taatttttgt gttaaacgat ggcaggcgct tgtgttatga cgattcacgg
    17221 caatttggta cgcttgaatt atcaactaaa gacacttact tacaaacaaa atcaatgcaa
    17281 aatgtaggaa gagaaccctt tgatattgat ccgatcgatt attttaataa aataagtaac
    17341 cgcactacac caattaaaca aactttactt gaccaaaagc tgatgagcgg gctcggtaat
    17401 atttatgttg atgaagtatt atacttaagt aaaattcatc cagaaacacc aacgaatatg
    17461 ttaggactta tggcaataaa tttacttatc gaaaatagta ttaaagtact taacgcggcc
    17521 ataaaaaaag gcggcacaac cgtaagaagc tatagtcctg ctgaaggagt gcacggtaac
    17581 ttccagcaag aattaaatgc ttatggtaag gcgggtgaaa ggtgtcctgt ttgtaatttt
    17641 aaattcaaga aaatatttgt tggtgggcgc ggtacaactt attgtccaag ttgtcaaaaa
    17701 aatcccgctg tgccattaac tgtcgccatt actggtcaaa aagcaagtgg gaaaacactt
    17761 atcgctaatt acttacggaa aaatggacac aaagttattg acactgataa actcgctaaa
    17821 gagctttata aagatattaa aattacaact gatttagaaa aacatctcgg cgttaattta
    17881 catgataaca ctacttttaa ccttcaatat ttacgaaatt atctagtgga aaatgaaaat
    17941 gaaataaaaa aactaaatga ctttattcat ccgcttgtaa aaaaagaaat tattgaaatt
    18001 attaagacga gtaatgaagc gattctttat tttgaagtac cgcttctttt ctcgcataaa
    18061 atatatgaat tatttaattt tatgattggg gttgaagtaa gcgttgaaaa gcaacttaag
    18121 aatttagaaa agcgcggaga tacgcataca attaacgctg atgaagcata tatgaaaaac
    18181 cgtcataaac tagacttaat catcgttaat gacggtagtg aacaagattt aatcgaaaag
    18241 tttaaagctt ttaaagttta gtgccttgta aataaatttc ttctttaata ttggctttaa
    18301 ttaagtcaac aatttgatgg ccacggacac tgcgaccaac ataaagactt cgtaagtcat
    18361 tcgtgcttag acccgctaaa ataatagtgg gctttttttg tctgactctc gctgctagaa
    18421 gtggaaaaat aattgattca atgacaatct tattaacttc ttcattaccg aagttatcaa
    18481 gcactaaaac atctaaatca atataatgtt gtaaataaga atcaacgata tcttttttat
    18541 cgtagtaatc agcaattgat gctctaacaa aagcagggaa attgacaact cccgctgttt
    18601 tatttttatc atcttcaagc attttagtca caaacggaat cgttgttttt aatttaccaa
    18661 tccccgctgt accataagca tatataaagt agatatcacc ttttaaaact ttcataagtt
    18721 cacgaataaa agctgcaaag cctcgtctag cgggtaagtc ttcgactctc aaatccaaat
    18781 aattttcggg aaaatcacga acaataaagc gcatcgctaa tgattgactt tctagataaa
    18841 ctgggcataa atgtctttcc actgtcacaa acggcgcgtc aatttgaata gtgagtgcat
    18901 gatgcttttc atcactaacg cagtgaccag ctttacgaca tttttctaca cttaagtaac
    18961 tttctaaata atcatttaaa agtccaacat tgttcttaat tatttctgga gttgcacctt
    19021 tttcttctaa aagtttaacg aaagatttat gactcataat tttgtcatac atttcttggc
    19081 gtatttcttc aacatttgtg gtgtcaatat ctaattctac ttttttcatt ttaacttctt
    19141 caattcatcc caaattggat catcaagatc atcaacttgc ggtgttgctt cttctttctt
    19201 gtcatcgtta aggattggcg cttttttagg tgcgcgtccg cgttttttta tcggccgttc
    19261 aagtgtatca agggcatcaa gcgcggtagt tactttgttt ctcgctagta aactagcgtt
    19321 tttctccact aaagcacgga taagttcatt gttattatga taaagcgtgt aataaacaag
    19381 ggcattaatt actggtgata ataaattata atcatgactt agcgtcgtta aaatattata
    19441 atcagcagga gcaattttcg tcccttcatt taagtattgt aaaaattcaa cgggactata
    19501 tgtttccatt aaattaatcc gcttaatttt ttgactttca cccgcaagtt cttgaactgg
    19561 acggcgtaat ggtttactta aaacggggta cttcacaagt tcttgcaagt gcttattcat
    19621 cgccacaaaa tcgactctag cgccaaaggg ttgtagagcg tcataaaaat caccgacacg
    19681 ctccgctgtt gctacttccg tcaaattata taaaccagcc atttgctcag cttttttaag
    19741 ctcttcagta cttagtgctg agggcaaaat tttacgttca cgcgttaaga tattaacaaa
    19801 agcagcgcga tcaaattcaa atttaatatt accgacaaca ttttctaatg gttcaccctt
    19861 atataggtca tcacctttaa tatcgaaaat acgatcagcg taaatttcgg caaaagttga
    19921 tgtaacttct tctttattta atgtttcacg atcgagcgta aataaagata ataagtcttg
    19981 tgtatggcgc ggaccaagca catcatttaa taaattcata aagatttgat cttgactaaa
    20041 cgctgctggg tcttttggtg catataattc taagacatat tcactaaatt cattatattc
    20101 aacataaaat gaccgcatta aaccaagacc ttctaattta ctaaaggcga tactaatttg
    20161 gtcataacta aacccaaaat gatcatgaat taaagtaaac gatagcggat caccttttct
    20221 aaaatcacgc attgtaaata aatagcgata taaggctgtc gcggcaaagc ccatgattgg
    20281 ttgatataaa atatctaaaa cttcaagatg taacgatgtt acataaccgc gaactttcac
    20341 actatagaaa tcattacttt taattaatcg catagtaaaa ttattataac acaatgaata
    20401 acgcaccgtt aaggtaagta tgtatttttt tacaaagaac aaataattac caaagttaac
    20461 acttttttgt agaatagaaa gaaaatattc gcactttctt taaaattgac tattataaat
    20521 aagtcaaagg tattgttaac atgataaaat ataaaatgta tggaggaagc aatatgacag
    20581 ttaaagtagc agtattaaca tcaggtggcg acgctccagg gatgaatgcc gcgattcggg
    20641 cagttatccg tagcggactc cttgctggct atgagatgtt tgctattttt gaaggctttc
    20701 gcggaattat cgaaaataaa atagttagtg tcgaccgtac tttcgttagt gaaattatta
    20761 tgcgcggtgg cactatttta aaaactgcgc gttttcccga gtttcgtgat tcaaaagttg
    20821 tcgaaaaagc agcggaaaac ttacgcttct atggtattac tcatctaatt gttattggcg
    20881 gtgacggtag ttatcgtggt gcatatgctt taagtaagca cggcattaaa attatcggga
    20941 ttcctgcgac aattgataat gatgtaatgg gtactgaaca aacaattggt tttgatacag
    21001 cgcttaatac aattacggaa gctgttgaca aacttcgtga tacttctagc agccatcacc
    21061 gctgttcaat ta
//
LOCUS       DAOSLP010000018        17059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_3549_length_17059_cov_8.767055, whole genome shotgun sequence.
ACCESSION   DAOSLP010000018 DAOSLP010000000
VERSION     DAOSLP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 17059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17059
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3549_length_17059_cov_8.767055"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3275)
                     /locus_tag="PK460_02695"
     CDS             complement(<1..3275)
                     /locus_tag="PK460_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOE54037.1"
                     /translation="MKLLKTVKIINWHYFWNQTLNFEQIVFLTGVNASGKSTLIDAMQ
                     VVLLGDTSGRHFNKAAAEKSNRTLRGYLRGELGDSLEGGFHYLRNGRFTSYIVLEFYD
                     DVNDDHFNLGIVFDVFDDGGVDHRFFMLEAPMPENEFIIDNVPMEYKDLNLFFQEHYP
                     NKYKFFDTNRQYQDALKRKLGNLKDKYFSLLKKAVSFSPITDITTFITEYVTEAQGNI
                     DIAPMQENILQYKKLEDEAALMRFKVERLNEIAKNYEVYKGHKEQLTLFEYVLEKATL
                     QNDLNRLATYDNQYLGYQKRLTDIESELADLDVDLAELNKRRVKLIQDSSSNDTYRIT
                     DEINEHKKVAEEKIKSLKFLNMQVRSDLENYVLTFDRLANEVLEKVSAMDEKRLKKDG
                     LYAREELLESAETIIKENHKITSVLNKDINELSETMLNDWRSAQAKFKQHLGAFNLSL
                     GRLINDYQVNEAILKQQELDLRGGGKPYEYSLVQIRNELQNKLSAKYGHKVEVSIFSD
                     LIDIRDAKWTNAIEGYLGGQRFHLFVEGEYFLDAYEFLKGLLQQYNFYGTNLVDVDAI
                     IERRFSAMPNSVAEEILTDHIGARAYADFLLGRLQKCNTIQEARNSGNGITPDTDLYR
                     NFTFGRINPRLYQQKYIGRRVEEADVNAKKAELAAHAETLRSYQELLQALLPGNDVEI
                     VNTNEIAALGSQVKRIQELPSLEQSIVFYDEELGRHDLTLIEGIHRRIGLVEADIKGI
                     EDQKSSLVEERGKISTNMQILKNERMPETKAIIRERQSKLEENYDAFFINDVAEPFFQ
                     NLIAEGKNPLDINAEYRQAFSNALYLSNSVFNQVKKLRKEYVVDYRLTYDVETLHNDD
                     FQMELNDLLEVKLPEYEEKIADAYRKAVKQFKDDFISKLRNAIETVEDQIAELNEALS
                     MSTFGEDSYQFTVKPSTVYRQYYDMIKDDLLLKVTDDDSEFFQKYDDVMKDLFAQIVL
                     AGDGDKNAAVLANVEKFTDYRNYLDFDLIVRNKDGHEQRLSRVLMKKSGGETQTPFYI
                     SVLASFAYLYRVNEPGELSNSARLIIFDEAFSKMDSGRIKESIRLLRRFGLQAIISAP
                     SDKV"
     gene            complement(3272..3910)
                     /locus_tag="PK460_02700"
     CDS             complement(3272..3910)
                     /locus_tag="PK460_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOE54038.1"
                     /translation="MFEEAFLQLTKADQDAFAKAINELLLNSFIVRDEFDRREKTIKI
                     NPTYRFIERYYDLINAYLSFAGWRVDKDLILGVFSLVNEYNHNRLRFDREVSLVLFVL
                     RLIYEEERKEGGHVGDSVYLTTPLVMKVMHDRGIGFPNKRLSGRLIGRCLKEIAAHNI
                     IARVSGSYDEGNVTFYLLPSITFAVDNEKIVAISEALEKLALRDAGTEGGNV"
     gene            complement(3912..5327)
                     /locus_tag="PK460_02705"
     CDS             complement(3912..5327)
                     /locus_tag="PK460_02705"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HOE54039.1"
                     /translation="MSLFTIIPSNFFSILASKNKEIYAEALLLLHHSIQDNELLIKKR
                     DFTRNLRDRLNELALSVDFDAEEEGDEDEIVNHSDKAAFIVRRLEECGWIDVEVNPDN
                     FEEYIALPSYSINVISLLNDLVKESTAEYVSLVHATYSELKLEDDMRDEFMYATLVRA
                     FENTKRLNVELITLAHSIRIFQNRLGKVFSTNNILSDYFDLYKTRVSDRYYHPLKTFD
                     SVAKFKRPILQILQKWLYDDDIRQQLVTQSLMWSRNKDAKAAEADIINKINYIADMYE
                     RVNETIDQIDQKHYEYTKASASKILYLNAADKSIKGHLDYIFKTYAETLSKGESVAPI
                     LNGINQSITFQQHGYITPDSLTLPIVRQLRAESIPMSLTDFDDVRDMLMQGFLDETSS
                     QFSNDAIFEFMDIAFDEESEVHISDIALPSFDAFLLLILATIKTSDEQCFYIIETEEG
                     SVSSYGYRLPNLTFVRKEITL"
     gene            5454..6521
                     /locus_tag="PK460_02710"
     CDS             5454..6521
                     /locus_tag="PK460_02710"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOE54040.1"
                     /translation="MANYELSRNTVSDLLSDNIQISPNTNEKLDYFRKAIKNTYPDYQ
                     KKFRHRARSFAVFAEIIIKRHNHTIKNNSIEHQKTYFKNDAYIYHIIEDFILAEEAKQ
                     NPEHTFTRDEYVDPTILQFENLIDHRYQNLLRYDFQKIKDPKLTLYNLTSRFFQELVS
                     GIMLLEREFYNDSFIIWRSLLETTTTLLILYENDNLVGKFNERRNIALMRVKVVDASR
                     QTLKSKAKETKQQLGFKGVPDYVAERYGWAGDLFKSRDYSLRTLLERINMVDLYSHYA
                     FASLFVHEYLISPEDLRLEIDFEKYLLSLYFKLYEAVRIKINDFTNDLDEVKKLEQGV
                     RKEVNNFKAQFNDFSTRIQTT"
     gene            6518..6967
                     /locus_tag="PK460_02715"
     CDS             6518..6967
                     /locus_tag="PK460_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54041.1"
                     /translation="MKHKRIYTSFRGLLLIYLPIYLLMSIIFYLIVGIKFPPTLNHYL
                     TVGGWTIITAIYLIFGYKNSYYELTKHEIVHHKGSNILYYKFDDIIYIDKVYSGKKLS
                     IRFFTRIGDERYLAHDPKKKVYNTMLERCTNLIDKEELLRRFPKIKY"
     gene            complement(6957..7652)
                     /locus_tag="PK460_02720"
     CDS             complement(6957..7652)
                     /locus_tag="PK460_02720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE54042.1"
                     /translation="MKTLKANSVDLIFADPPYFLSNDGLSIRSGKVVSVNKGEWDKRD
                     NYDSTYMFTYSWLKECHRILKPTSSIWVSGTHHNIFEIKKVMDDLGFRIINLVIWQKT
                     DPPPLIYKNKLKFSHEILIWAKKGRSHFFNYDLAYSLRNSELGDVWKFSAVQNHEKKF
                     GYHPTQKPERLLERIIRLTTNKNDVVLDPFMGSGTTCFVAKKLQRQYIGIELEEEYFS
                     IAKRRIDSIKANT"
     gene            complement(7720..8580)
                     /locus_tag="PK460_02725"
     CDS             complement(7720..8580)
                     /locus_tag="PK460_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOE54043.1"
                     /translation="MSRNFEEWLSTFRETIATWDYYTDFNKVYRNVDAVKVELNILNS
                     LIGSDDIRNEFISLVESYPHVLQVVPLLLAKRENTIKINTSETMYDFDFKNTNYSIEE
                     YANFMDKTGLFNLLKNRILRSLLDYVTGIEVGMDTNARKNRMGKAMEDLVEEHLKKKG
                     FVRDVTYFKEMSRSEIQSKFGLDLSNITNEGKAEKRFDFVIKTKDHVYAIETNFYSSS
                     GSKLNETARSYELLARESATVQGFTFIWITDGLGWRSARNNLRQTFEVTRHIYNIADL
                     ENDVLAVVLK"
     gene            complement(8612..9403)
                     /locus_tag="PK460_02730"
     CDS             complement(8612..9403)
                     /locus_tag="PK460_02730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE54044.1"
                     /translation="MNIINVGKSALKINDDDFILVQGNALRVLKKIAPKSVDVIFADP
                     PYFLSNDGITCVNGKMVSVNKALWDKSISATDKLNFNRKWIRYCRDALKDDGTIWISG
                     TMHNIYSIGVALELEGFSIINNITWRKPNPPPNIACRAFTHSTETVLWARKIINNRKK
                     GKHFFNYALMKEINGGKQMKDVWELKGALKKEKEHGKHPTQKPFELLERIILASSNEG
                     DIILDPFAGSGTTGIAAKRLKRRFIGIEIEKEYIGIIKKRYKAIQ"
     gene            complement(9405..10466)
                     /locus_tag="PK460_02735"
     CDS             complement(9405..10466)
                     /locus_tag="PK460_02735"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOE54045.1"
                     /translation="MDLINKNIGEKIKSRRLRLGYSQLMLSELAHVSRDQISRIESGK
                     INFTLATLFKICRALRIKPHKLLKDAEQEEYIKSGDLRPFVKWAGGKTQILDELMQRI
                     PNEFNNYFEPFVGGGAFFLKLEHPRSVINDINSELMTAYSVIKDKEKFIALKKLLLQY
                     EEKHNEEFYYEVRARDRDPSFSTLSDVEIAARFIYLNKNCFNGLYRVNSKGYFNVPFN
                     KSEKVKTFDEDNINKLHKYLSLPSIKICCEDYKNMTNKAKKGDFVYFDPPYDTLKDDT
                     FTAYDKEGFGRNEQIELSEEFKKLHKKGVKVMLSNHNTPLINELYKEFNITIIHARRA
                     INSRGSGRGKIEEVIITNY"
     gene            10633..10824
                     /gene="rpmB"
                     /locus_tag="PK460_02740"
     CDS             10633..10824
                     /gene="rpmB"
                     /locus_tag="PK460_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOE54046.1"
                     /translation="MARHCPVTGKGPLSGNKRSHSLRATRRRWGVNLQNYKVEVDGKV
                     QTVRMSARAYRSLNKSAKV"
     gene            complement(10874..11527)
                     /locus_tag="PK460_02745"
     CDS             complement(10874..11527)
                     /locus_tag="PK460_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54047.1"
                     /translation="MDKSIVHIAFFSSLSLFVITLIFQLSLYRTKQNRKFSFRNELPF
                     ELVQGADIKFINYHYVLLFLVTIANLLFAFKYLDHIYNWYEYLLVGSLVLSAIMLYLI
                     FFIKVFEIKKHIIVVILQALSVVTSYLSFGLFAHISPFGKQNIVFGIFGYLFALIGML
                     VLLNPRLRKWPIMDKVLQQDGTVLILRPRYFMLALYEWGFIAAQFLLMIVMYAYLYV"
     gene            complement(11520..12185)
                     /gene="rpe"
                     /locus_tag="PK460_02750"
     CDS             complement(11520..12185)
                     /gene="rpe"
                     /locus_tag="PK460_02750"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOE54048.1"
                     /translation="MKQKVYVAPSLLAADFKNLHRELLILQQNKVKYLHFDVMDGHFV
                     PNLSFGIPILEALKPHYIFIYDVHLMVTNPEFIAPKFVEAGADIVTFHLEAMKTGNDV
                     VTLIKELRLMGVKVGISIKPDTPVEDLAPILKDIDLVLVMSVEPGFGGQSFLRSALHK
                     IEWLDEERKKYKYNYLIEVDGGINAETGALCAEAGADILVAGSYLFGKEDINERIKGL
                     RNG"
     gene            complement(12182..13042)
                     /gene="rsgA"
                     /locus_tag="PK460_02755"
     CDS             complement(12182..13042)
                     /gene="rsgA"
                     /locus_tag="PK460_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOE54049.1"
                     /translation="MAGLVINKQKSSFIVEKNGETYVTKPSGNLIKSRQTIYVGDYVE
                     IDTENNLISELLPRRNFLVRPPIANVDIVLIVMSMVQPDFSLFLVEKFLSYANFAEIK
                     PLVIITKQDKLDTDDLLKEVQDNLDKLHVKNYVVNNKSGEGLDAVRKYLHGKVVALMG
                     QSGVGKSSFINALTDYDREVGKFSISINRGRHVTKEVIMLPFNAGYLVDTPGFSSFEL
                     PLTKGDLAENFPGFKKYIGTCKFNNCLHLSEIGCTVKADVEAGLISEESYNNYVMISD
                     ELKLYSEDYK"
     gene            complement(13043..13930)
                     /locus_tag="PK460_02760"
     CDS             complement(13043..13930)
                     /locus_tag="PK460_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOE54050.1"
                     /translation="MIRIGDLIDNRYRIVSLLGEGGMGTVYEAVDIITKRNVALKFLK
                     ANVARDKVNLARFEIEAKATATLNHPNIVRVISVGKYENLPYMVNELIVGNNLEDELQ
                     TRGRYTFLEAADIIDQLCNAVSLAHKNGVIHRDIKPSNIYITKEGLVKLGDFGIARYS
                     DSKRVTSNTNIVGSAHYLAPEVCRGGEASPRSDIYALGINFFELITGHVPFDGDSNVA
                     IAVKQIKEKFPSPRKYVKNTPKAIEKIIKKCCKKNPRERYPDVESLQQDILQLKTNPK
                     LLRPRQSIWVRWFGFVSED"
     gene            complement(13923..14675)
                     /locus_tag="PK460_02765"
     CDS             complement(13923..14675)
                     /locus_tag="PK460_02765"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOE54051.1"
                     /translation="MATTVKGTFAAKSDRGLVRLHNEDRAAVLLNQKGDLLFVVCDGL
                     GGHNKGDFASKLAIDMLMTAFANNRGFLTTNSIKRFFNKNIRAINKAIFEESILSPDM
                     NNMSTTLNAVVLTRSKLCNVNIGDSRFYLCTDDEMYLMSEDQTVVGYLVRTNQITPEE
                     ALVHPKRHILTNALGTMPTVTFDFRKFQYRGERILVCSDGLYNMLDSEDILGILRTTQ
                     TAEFKVDALVALANHRGGADNIGVALWEPDND"
     gene            complement(14659..15723)
                     /gene="rlmN"
                     /locus_tag="PK460_02770"
     CDS             complement(14659..15723)
                     /gene="rlmN"
                     /locus_tag="PK460_02770"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOE54052.1"
                     /translation="MSKKLNFYDYSLPKLQDFLLNEGFKKYSATQMFEWVYLKDELDF
                     NNMSNLALVTREFMINNFTFALPTVHELQESKDGTVKLLLKLGDGSLVETVLMRYRYG
                     NSVCVSTQVGCNMGCRFCASGLLKKKRDLTPGEMLGQVLMINEYLKGRGELKLTTHVV
                     LMGTGEPFDNFENILDFIDLLNNPKSLAIGARHITVSTVGLVPKIKEFADLKLQVKLA
                     ISLHAATDEKRNQIMPINNAYPLKEVLSAVKYYESKTNKRVTYEYIMLNDFNDTLEDA
                     RALVNLVKGTNGFVNLIAYNPIAEFDYEPSTRDHVYKFANYLKSMGVQTTVRKEFGSD
                     IDAACGQLRAKVEKKRGNNS"
     gene            complement(15707..16924)
                     /locus_tag="PK460_02775"
     CDS             complement(15707..16924)
                     /locus_tag="PK460_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54053.1"
                     /translation="MIKHILDTTEKIIDGSLPYQQFSKDLKQDNSYSQSEKHQFRTLV
                     NALLNRYFRYLYSLTEGEETTLTQFEVLALSLFSFNREKKVVPLSYEEFLAALPTDIQ
                     VKFEQEYPDESFPIRIPKHFHSENPALSLAARFSVPLALTEQLIADIGRKNLLKFLTN
                     RYRETYGLINQNVISDEDFFANMPLFIRGLKPGEYEYKGKEYIKKTPAYNNNEIVIAS
                     QTLLDVVDIIESKKPKNLLFVQHEDTSLMLVLALKYPEIQIHFYKDDNRSKYIVRHLV
                     RKFSLENVTYVDKITQTYDFVLTTLQSSNINGDIRHRDFYFRLPDNLQFFAAKGREEL
                     EELKDYVNEDGHLMFITTTVLRFETHLQAVGFLRDYPEFELILEKQHFHFIPNNETLY
                     YALLKKGAHVEEA"
BASE COUNT         5493 a         3215 c         2636 g         5715 t
ORIGIN      
        1 actttatcac ttggggcact aataattgct tgtaagccaa agcggcgtaa taagcgaatt
       61 gattctttaa tccgcccact atccatttta ctaaacgctt cgtcaaagat aataagacgg
      121 gcgctgttgc taagttcacc tggttcatta acgcgataga gataagcaaa actagcaagt
      181 acactaatgt agaacggggt ttgtgtttcc ccaccagact tcttcattaa tacgcgtgat
      241 aagcgttgtt cgtggccatc tttattgcgg acaattaagt caaagtctaa ataattacgg
      301 taatcggtga atttttcaac attagcgagc actgccgcat ttttatcacc gtctcccgct
      361 aacacgattt gcgcgaataa atctttcatc acatcgtcat atttttggaa gaactcacta
      421 tcgtcatccg ttactttaag taataaatcg tctttaatca tatcgtaata ttgccgatat
      481 actgtacttg gtttaaccgt gaattgatac gagtcttcac caaatgtaga catactaagt
      541 gcttcgttaa gttcagcaat ttgatcttca accgtttcaa ttgcattccg caatttcgaa
      601 atgaagtcat ctttaaattg tttaaccgct ttacgatatg catccgcaat tttttcttcg
      661 tactctggta atttaacttc taacaagtca tttaattcca tttggaagtc gtcattgtgt
      721 aaagtttcaa cgtcatatgt taagcgataa tcaacaacat actctttacg cagtttcttc
      781 acttgattga agacactatt tgagaggtag agcgcattgc taaatgcttg ccgatattca
      841 gcattaatat caagcggatt cttaccttcg gcaattaagt tttggaagaa tggttcggcg
      901 acatcgttaa taaagaaagc atcataattt tcttcaagtt tactttgccg ttctctaatg
      961 attgcttttg tttccggcat tctttcattc tttaaaattt gcatgtttgt ggaaatctta
     1021 ccgcgctctt ccactaaact tgatttttgg tcttcaatac ctttaatatc tgcttcgact
     1081 aagccaatcc ggcgatgaat cccttcaatt agtgttaagt catgacggcc aagttcttca
     1141 tcataaaaga caatgctttg ttcaagactt ggtaattctt gaatacgctt aacttgactg
     1201 ccaagggcgg cgatttcgtt agtgttaaca atttcgacat cattgcctgg taagagtgct
     1261 tgcagtaact cttgataaga ccgtaatgtt tcagcgtgag cagcaagttc agctttcttc
     1321 gcgttaacat ccgcctcttc tacgcggcgt ccaatatatt tttgttggta taagcgcgga
     1381 ttaatacgcc caaaagtaaa gttccgataa agatcagtat caggcgtaat accattacca
     1441 ctattgcgtg cttcttgaat tgtattacat ttttgtaaac gaccaagcaa gaaatcagcg
     1501 tatgctcttg ccccaatgtg gtcagttaat atttcttcag caacagaatt tggcattgca
     1561 ctaaagcggc gctcaattat cgcgtcaacg tccactaaat ttgtgccata gaaattatat
     1621 tgttgtaaaa gtccttttaa aaattcataa gcatcaagga aatattcacc ttcgacaaaa
     1681 agatggaagc gttggccacc taagtaacct tcaatcgcat ttgtccactt cgcatcgcga
     1741 atatcaatta agtcactaaa aatacttact tcgactttat gaccatattt tgccgaaagt
     1801 ttattttgta attcattgcg aatttgcaca agactatatt catatggttt accaccaccg
     1861 cgtaaatcta gttcttgttg ttttaaaatt gcttcattta cttgatagtc attaattaaa
     1921 cgaccaagac ttaagttaaa agccccaaga tgttgcttaa atttcgcttg agcactgcgc
     1981 caatcattaa gcattgtttc actaagttca ttaatgtctt tatttaaaac actagtgatt
     2041 ttatgatttt ctttaataat tgtttcggcg ctttcaagta gttcttcgcg cgcatataat
     2101 ccgtctttct ttagccgttt ttcatccatc gcactaactt tttctaaaac ttcattagcg
     2161 agtctatcaa atgttaggac ataattttct aagtcactac ggacttgcat atttaagaat
     2221 ttaagacttt taattttttc ttctgcgact tttttatgtt cattaatttc atcagtgatg
     2281 cgataagtat cattacttga actatcttga attaatttaa cacggcgttt attaagttca
     2341 gcaaggtcaa catctaaatc agcaagttca ctttcaatat ctgttaagcg tttttgataa
     2401 cctagatatt gattatcata agttgctagt cgatttaagt cattttgtag tgttgctttt
     2461 tctaagacat attcaaaaag tgttaattgt tctttatgac ctttgtatac ttcatagttt
     2521 ttagctattt catttaagcg ttcaacttta aaacgcatca gcgctgcttc gtcttcaagc
     2581 tttttatatt gcaaaatatt ttcttgcatt ggtgcaatat caatattgcc ttgtgcttca
     2641 gtgacatatt cagtaataaa agtcgtaata tccgtaatgg gtgagaaact aacagctttc
     2701 tttaagagac taaaatactt atcttttaaa ttaccaagtt tccgtttaag ggcatcctga
     2761 tattgacggt tcgtgtcaaa gaatttgtat ttatttggat aatgttcttg gaaaaataaa
     2821 tttaaatctt tatattccat cgggacatta tcgatgataa attcgttttc cggcattggt
     2881 gcttctaaca taaagaagcg atgatcaacc ccgccgtcat caaacacatc aaagacaatc
     2941 cctaagttaa agtgatcatc attaacatca tcataaaact ctaaaacaat gtaagaagta
     3001 aagcgtccgt tccgtaaata gtggaatccg ccttctaacg agtcgccaag ttccccgcgt
     3061 aaatatccgc gtaatgtccg gtttgatttt tccgcggcgg ctttattaaa gtggcggccg
     3121 gaagtatcac caaggagcac aacttgcatt gcgtcaataa gtgttgactt cccgctagcg
     3181 ttcaccccag tcaggaagac gatttgttca aaatttaagg tttgattcca aaaatagtgc
     3241 caattaataa ttttgactgt ttttaataat ttcatacatt acctccctcg gtgcctgcat
     3301 cgcgtaaggc taatttttcg agagcttcgc taattgcgac aattttttca ttatcaacgg
     3361 caaaagtaat cgatggtaat aagtagaaag taacattacc ctcatcataa cttcccgaga
     3421 ctctagcaat aatattatgt gccgcgattt cttttaaaca acgaccaatt aagcgcccac
     3481 ttaggcgttt atttggaaaa ccaatgccac gatcatgcat tactttcatc acaagtggtg
     3541 ttgttaagta cacagaatcg ccaacatgtc ccccttcttt ccgttcttct tcataaatta
     3601 ggcgcaagac aaataacact aagcttactt cacgatcaaa gcgtaagcgg ttgtgattat
     3661 attcattaac gaggctaaat acacctaata ttaaatcttt atcaacacgc cagcctgcaa
     3721 aacttaaata ggcattaatt aagtcgtaat agcgttcaat aaaccgataa gtcggattaa
     3781 ttttaattgt tttttcgcga cgatcaaatt catcacgcac gataaaacta ttaagtaata
     3841 attcattaat tgctttagca aaggcgtctt gatctgcttt tgttaattgt aaaaaggctt
     3901 cttcaaacat attataaggt aatctccttt cgtacgaatg ttaagttcgg taatcggtaa
     3961 ccatagctac taacactacc ttcctcggtt tcgattatgt aaaaacattg ttcatcactc
     4021 gttttaattg ttgctaaaat taaaagtaag aatgcgtcaa atgacggtaa agcaatatca
     4081 ctaatatgta cttcactttc ttcatcaaac gcgatatcca taaattcaaa gattgcatca
     4141 ttagaaaatt ggctacttgt ctcatcaagg aacccttgca ttaacatatc acgcacatca
     4201 tcaaagtcag taagtgacat cggaatactt tcagcgcgta attgacgaac aattggtaag
     4261 gttaatgaat caggggtaat gtaaccatgt tgttggaatg taattgattg attaatcccg
     4321 ttaagaatcg gcgcgacact ttcaccttta cttaatgttt cagcataagt cttaaaaata
     4381 taatcaaggt gacctttaat cgatttatcc gcagcattta aatataaaat ctttgaagca
     4441 ctagctttcg tgtattcata gtgtttttga tcaatttggt caattgtctc attaactctt
     4501 tcatacatat cagcaatgta gttaatttta ttaataatat cggcttcggc cgcttttgcg
     4561 tctttgttgc ggctccacat taatgactgt gtcactagtt gttgacggat atcatcgtca
     4621 tacaaccact tttgtaaaat ttgtaaaatt ggtcgcttaa atttagcgac actatcaaat
     4681 gttttaagcg ggtgataata acggtcactc acgcgcgttt tatataagtc aaaataatca
     4741 cttaaaatgt tattagtgct aaatactttt cctaagcgat tttggaaaat gcggatgctg
     4801 tgcgctaagg tgataagttc gacattaagg cgttttgtat tttcaaatgc ccgcactaat
     4861 gtcgcataca taaattcatc gcgcatatca tcttctaact taagttcgct ataagtcgcg
     4921 tgcacaagcg aaacgtattc agcagtgctt tctttcacta aatcgtttaa taaggaaata
     4981 acattaatac tataacttgg aagcgcaatg tattcttcaa aattatcagg attaacttca
     5041 acatcaatcc agccgcattc ttctaaacga cgaacgataa atgccgcttt atcgctatga
     5101 ttaacgattt cgtcttcatc gccttcttct tccgcgtcaa aatcaacact aagcgctagt
     5161 tcatttaaac ggtcacgcaa attacgagta aaatcgcgct tctttattaa taattcattg
     5221 tcttgaattg aatggtgtag taaaagaagt gcttcagcat atatctcttt attcttactc
     5281 gcaagaatcg agaagaagtt actaggtatg attgtaaata gactcaaaaa aaataatctc
     5341 cttcggttat atgttttatg ttaacatata aaaaaatgaa ttggtattaa acttgtataa
     5401 taaaagagaa atatttaatt tcgtttaaaa taataataga aaggcggtaa catatggcaa
     5461 attatgaact tagtcgcaat actgttagtg accttttaag tgataatatt caaatttcac
     5521 caaacacgaa tgagaaactt gattacttcc gtaaagcaat taaaaatact tatcctgatt
     5581 accaaaaaaa gtttagacat cgtgctcgtt catttgcagt atttgcggaa atcattatca
     5641 aaagacataa tcatacgatt aaaaataaca gcattgaaca tcaaaaaaca tattttaaaa
     5701 atgatgctta tatttaccat atcattgaag attttatcct agcggaggag gctaaacaaa
     5761 atcctgaaca cacttttaca cgtgatgaat atgttgaccc gacaatctta caatttgaaa
     5821 acttaattga tcatcgttat caaaatttat taagatatga ttttcaaaaa ataaaagatc
     5881 caaaactaac tctttacaac ttaacaagcc ggttctttca agaattagtg tcaggaataa
     5941 tgctcttaga acgcgaattt tataatgact ccttcatcat ttggcgcagt ctcttagaaa
     6001 caacaacgac cttgttaata ctttacgaaa acgataatct agttggtaaa tttaatgaga
     6061 gacgcaacat tgcgctgatg cgtgttaagg tcgtagatgc gagtcggcaa acactaaaaa
     6121 gcaaagcaaa agaaactaaa caacaactag gctttaaagg tgtgccagat tatgttgcag
     6181 aacgttacgg ttgggcgggt gatttattta aatcgcgcga ctattcctta cggacactac
     6241 ttgaaagaat taacatggtt gacctttatt cgcattatgc ctttgcctcg ttattcgtcc
     6301 acgaatattt aataagtcca gaagatttac ggctcgaaat agattttgaa aaatatttat
     6361 tatcacttta ctttaaactt tatgaagccg tgcggattaa aataaatgac ttcacaaatg
     6421 accttgatga agttaaaaaa ttagaacaag gagtgcggaa agaagttaat aattttaaag
     6481 cacaatttaa tgactttagt acgcgaattc aaacaacatg aaacacaaac gaatttacac
     6541 aagttttaga ggattacttt taatttattt accaatatat ttattaatga gtatcatctt
     6601 ctatctcata gttggtatta agtttccacc aaccttaaat cactatctaa cagtgggtgg
     6661 ctggacaatt attactgcaa tatatttaat atttggttat aaaaatagtt attatgaact
     6721 aacaaaacat gaaatagtgc accataaagg tagcaatatc ctttattata aatttgatga
     6781 catcatttat attgataaag tatattccgg gaagaagctt agtatccgct tctttacgcg
     6841 aattggcgat gagcgctatt tagcgcatga tccaaagaaa aaagtgtata acacgatgct
     6901 tgaacgctgc actaatctta tcgataaaga ggaattatta cgcagattcc caaaaattaa
     6961 gtattagctt ttatactatc aatccttctt tttgcaattg aaaaatattc ttcttctaat
     7021 tcaataccaa tatattgacg ctgaagtttt ttagcgacaa aacaagtagt gccactaccc
     7081 ataaatggat ctagtaccac atcatttttg tttgtggtta atcgaatgat tctttctaat
     7141 aatctttcag gcttttgtgt gggatgataa ccaaattttt tctcatgatt ttgtactgcc
     7201 gaaaatttcc aaacatcgcc aagttcacta ttacgcaaac tataggctag gtcataatta
     7261 aaaaagtgtg accttccttt tttagcccaa attaatattt catgcgaaaa ctttaatttg
     7321 tttttataaa ttaatggtgg tgggtctgtt ttttgccaga ttactaaatt aattatccga
     7381 aaaccaagat catccatcac ctttttaatt tcaaaaatat tatgatgagt gccacttacc
     7441 cagattgaac ttgttggttt taaaatcctg tgacattctt ttaaccaact ataagtgaac
     7501 atgtatgtac tatcgtaatt atctcttttg tcccattcac ctttatttac tgacacaacc
     7561 ttaccacttc taatacttaa accgtcgttg gaaagaaagt agggaggatc agcaaaaatt
     7621 aaatctacac tatttgcttt tagtgttttc attactttcg tgcaatcacc taaatacaac
     7681 atactaataa ttacaaatgt aattactaat atttctcatt tattttagga caactgctaa
     7741 aacatcattt tctaaatcgg caatattata aatatgacga gtaacttcaa atgtttgacg
     7801 gagattattt ctagccgaac gccaccctaa gccgtctgtt atccaaataa aggtaaaacc
     7861 ttgaactgtt gcactttctc ttgctaaaag ttcataactt ctggcggttt cattaagttt
     7921 tgagcctgaa gaagaataaa aattcgtttc aatcgcatag acatggtctt ttgtttttat
     7981 tacaaaatca aaacgctttt cagcttttcc ctcgttagta atattagata aatctaatcc
     8041 aaatttactt tggatttcac ttcgactcat ttctttaaaa taagtaacat cgcgcacaaa
     8101 gcccttcttt ttaagatgtt cttccactaa gtcttccatt gctttgccca tacggttctt
     8161 acgggcgttc gtatccatgc cgacttctat tccagtaaca taatcgagca agctacgcag
     8221 aatacgattt ttcagcaagt taaataatcc ggttttatcc ataaagtttg cgtattcttc
     8281 aatggaatag ttcgtatttt taaaatcaaa gtcatacatt gtttccgaag tattaatctt
     8341 aattgtgttt tctcttttgg ctaaaagtaa aggcactact tgtaaaacat gcggataact
     8401 ttcaacaaga ctaataaatt cattgcgaat atcatcactg ccaataagac tattaagaat
     8461 atttaattcg actttgactg catctacatt acgataaacc ttattaaaat cagtataata
     8521 atcccatgta gcaatagttt ctctaaaagt gcttaaccac tcttcaaagt ttcgactcat
     8581 aatcattgcc tcctatttta ataatacaaa cttattgtat tgctttataa cgttttttaa
     8641 taattccaat atattctttt tcaatttcta tcccaatgaa acgtcttttt aatctttttg
     8701 cggcaatacc agtagtgcca cttccagcaa agggatcaag tataatatca ccttcattac
     8761 ttgatgcaag aataattcgt tctagtagtt caaacggttt ttgcgtagga tgcttaccgt
     8821 gttctttttc tttttttagc gcacctttta attcccacac gtccttcatt tgcttaccgc
     8881 cgttaatttc tttcattagt gcataattaa agaaatgttt gccctttttt cggttattaa
     8941 tgattttacg agcccaaagc acggtctcgg ttgaatgggt aaaagcgcga caagcaatat
     9001 tcggtggtgg attaggtttg cgccatgtaa tgttgttaat aatagaaaat ccttctaatt
     9061 caagtgctac cccgattgaa taaatattat gcattgtgcc actaatccag attgttccat
     9121 catcctttaa agcatcgcga caatatctaa tccatttacg gttgaaattt aatttatctg
     9181 tcgcgctgat acttttatcc cacaaagcct tattaacaga aaccatttta ccgtttacac
     9241 atgtaatacc gtcatttgat agaaagtaag gtgggtcagc aaaaataaca tcaactgatt
     9301 ttggggctat tttctttaaa acgcgcaaag cattaccttg cactaaaata aaatcatcat
     9361 cgtttatctt aagtgcgctt tttccaacat taatgatatt cattttagta attagtaata
     9421 atcacttctt caattttacc gcgcccacta ccacgtgaat taatagcgcg acgagcatga
     9481 ataatcgtaa tattaaactc cttataaagt tcattaatca gtggtgtgtt atgatttgaa
     9541 agcataacct tcacaccttt tttatgtaat tttttaaatt cttccgaaag ttcaatttgt
     9601 tcattacgac caaagccttc tttatcatag gcagtgaatg tatcatcctt aagcgtgtca
     9661 taaggtggat caaagtaaac aaaatcacct tttttagctt tgttagtcat attcttgtaa
     9721 tcttcacaac atattttaat actaggtaat gacagatatt tatgtagttt atttatatta
     9781 tcttcatcga aagtttttac tttttcactt ttattaaatg gcacattaaa ataacctttg
     9841 gaattcactc ggtacaaacc attaaaacaa ttcttattta agtatataaa cctagcggca
     9901 atttcaacat cacttaaagt cgaaaatgaa gggtcacggt ctctcgcgcg aacctcatag
     9961 taaaattcct cattatgttt ttcttcatat tgtagtaata gtttcttcag tgcaataaat
    10021 ttttctttat ctttaataac actataagca gtcattagtt cactgttaat atcgttaatg
    10081 acggaacgcg ggtgttcgag ttttaaaaag aacgcgccac cccccacaaa aggttcaaag
    10141 taattattga actcattagg aattctttgc ataagttcgt ctaatatttg cgttttacca
    10201 cccgcccatt taacaaaagg acgaaggtcc ccacttttaa tatattcttc ttgttcagca
    10261 tctttaagca atttatgagg tttaattcta agcgcccgac aaattttaaa tagtgtcgct
    10321 aatgtaaaat ttatcttccc gctttcaatc cgtgaaattt ggtcacggga tacatgagca
    10381 agttccgata acattaattg tgagtagcct aaacgcaatc ttctgctttt tattttttca
    10441 ccgatattct tattaattaa atccatagag agagtataac ataatgtgga ctaaaataca
    10501 cattaataac aaaggtattt attaacaata tgtgcaaatt tgcaataatt ttcaaaattt
    10561 aactttttat atttgcaaat ttataacatt aatgttatct taatgtagca tttattgaat
    10621 ggggaggaaa ttatggctag acattgtcca gtaacaggca aaggaccctt aagtggcaat
    10681 aaacgcagtc actctcttcg cgcaactaga cggcgctggg gcgtaaactt acaaaactat
    10741 aaagttgaag ttgatggcaa agtccaaacc gtccggatgt ctgcaagagc atatcgctca
    10801 cttaacaagt ccgccaaagt ataaaaaaag ctcacaacaa agtgagtttt ttttattatc
    10861 ttcttctttc tacttaaaca taaagataag cgtacattac aatcattaat aaaaactgtg
    10921 cggcaataaa accccattca taaagcgcta gcataaaata gcgcggtcgc aaaataagta
    10981 ccgttccatc ttgctgtaaa actttatcca taatcggcca tttgcgaagt cgtggattaa
    11041 gtaacactaa catcccaata agcgcaaata agtaaccaaa aataccaaag acgatatttt
    11101 gtttaccaaa tggactaatg tgcgcaaata aaccaaatga aagataactt gtgacaacag
    11161 ataaagcttg taagataaca acgataatat gttttttaat ttcaaatact ttaataaaga
    11221 aaataagata gagcattatc gcacttaata cgagtgaacc gactaataaa tattcgtacc
    11281 aattataaat atgatctaaa tatttaaagg caaataataa attagcgatc gtcacaagaa
    11341 acaataatac atagtgataa ttgataaatt tgatatcggc gccttgtaca agttcaaacg
    11401 gcagctcatt acgaaacgaa aacttccgat tttgtttagt gcgataaagt gataattgaa
    11461 aaattaaagt aatcacaaac agtgataatg atgaaaaaaa agcaatatga acaatactct
    11521 tatccattac gtaatccctt tatcctttca ttaatgtctt ctttaccaaa taagtatgaa
    11581 cccgcgacaa gaatatcagc gcctgcttca gcacaaagtg ccccagtttc agcattgata
    11641 ccgccatcaa cttcgatcaa gtaattatat ttatactttt tgcgctcttc atcaagccat
    11701 tcaattttat gtaaagcact acgtaagaaa ctttgtccac caaagcctgg ttcaactgac
    11761 atcacaagca ctaaatcgat atcttttaaa atcggcgcta agtcttcaac aggtgtatca
    11821 ggtttaatac taatacctac ctttacaccc atcagtcgta attctttaat tagtgtaaca
    11881 acatcattac cagttttcat tgcttcaagg tgaaaagtaa cgatatccgc tccagcttcc
    11941 acaaattttg gagcaataaa ttcaggatta gtaaccatta aatgtacatc ataaataaat
    12001 atataatgag gtttaagtgc ttctaaaatt ggaatcccaa atgataaatt tggaacaaaa
    12061 tgtccatcca taacatcaaa atgtaaatat ttaaccttgt tttgctgcaa aattaatagt
    12121 tcgcgatgta aatttttgaa atccgcagct aataaagatg gggcaacata aactttctgt
    12181 ttcatttata atcctcacta tataacttta actcatcact tatcataaca taattattat
    12241 aactttcttc actgataagc ccagcttcaa catcggcctt aacggtacag cctatttcac
    12301 taagatggag acaattatta aatttacaag taccaatata ctttttaaat cccgggaagt
    12361 tttcggcaag gtcgcctttc gttagcggta attcaaagga agaaaagcct ggtgtatcaa
    12421 ctaaatatcc ggcgttaaac ggaagcatta ttacttcttt agtgacatgt cgaccacggt
    12481 taatgctaat tgagaactta ccaacttcgc ggtcataatc tgttaaagcg tttataaaac
    12541 ttgattttcc gacaccagat tgccccataa gtgccacaac tttgccatgc aaatatttgc
    12601 gcacagcatc aagtccttca ccacttttat tattgacaac ataattttta acgtgtagtt
    12661 tatcaagatt atcttgtact tcctttaaca agtcatcagt gtcaagttta tcttgctttg
    12721 taatgatgac tagtggctta atttccgcaa aattagcgta actaagaaac ttttcgacta
    12781 aaaatagact gaaatcaggt tgcaccattg acatgacaat taacacaata tcaacattgg
    12841 caattggtgg tcttactaaa aagtttcgcc gtggtagaag ctcactaatt aaattatttt
    12901 ccgtatcaat ttcaacataa tccccgacat aaatagtctg gcgcgattta attaaattac
    12961 cgcttggttt agttacataa gtttcaccat ttttctctac gataaaactt gatttttgtt
    13021 tattaataac taatccagcc atctagtcct cagagacaaa cccaaaccag cgcacccaaa
    13081 ttgattgccg tggccgtaaa agttttgggt tagtcttaag ttgtaatata tcttgttgta
    13141 atgattcaac atcaggatag cgttcccgtg gatttttctt acaacatttt ttaataattt
    13201 tctcaatagc tttcggggta tttttaacat acttacgcgg acttggaaac ttctctttaa
    13261 tttgttttac cgcaattgcc acattcgaat caccgtcaaa gggaacatga cctgtaatta
    13321 attcaaagaa attaatacct aaagcataaa tatcactgcg tggtgaagct tcgccaccac
    13381 gacacacttc gggcgctaag taatgggcgc taccaacaat atttgtgtta cttgtgacac
    13441 gctttgagtc actataacga gcaataccaa agtccccaag tttaacaagt ccttctttag
    13501 taatataaat attacttggt tttatatcac gatgaattac cccgttttta tgcgctaaac
    13561 taacagcgtt acacagctga tcaataatat cagcagcttc taaaaatgta tagcgaccgc
    13621 gcgtttgcag ttcatcttct aagttattac cgactattaa ttcattcacc atgtaaggta
    13681 aattttcata cttgcctaca ctaatgacac gaacaatatt tggatgattt aaagtcgcag
    13741 ttgctttggc ttcaatttca aaacgggcaa gattcacttt atcacgagcg acattagcct
    13801 ttaaaaactt cagcgcgaca ttacgtttgg taatgatgtc gactgcttca taaactgttc
    13861 ccattccgcc ttcaccaagc aaacttacaa tccgatatcg attgtcaatt aaatcaccta
    13921 ttctaatcat tatctggctc ccataatgct actcctatat tgtcggctcc accgcgatga
    13981 ttggcgagag cgacgagtgc gtctacttta aattccgctg tttgtgttgt gcgtaatatt
    14041 cctaaaatat cttcgctatc aagcatgttg taaagaccat cactacacac taaaattcgt
    14101 tcaccccgat attgaaactt cctaaaatca aaagtaacag ttggcatcgt accaagcgca
    14161 tttgttaaga tgtgacgttt ggggtgcact agtgcttctt cgggggtaat ttgatttgtg
    14221 cgcactaaat aaccgacaac tgtttggtct tcactcatta aatacatttc atcatccgta
    14281 cataaataaa aacgactatc accaatatta acattgcaca attttgaccg cgttaatact
    14341 acagcattta atgttgttga catattgttc atatctgggg ataaaatact ttcttcaaag
    14401 atggctttat taatagcgcg aatattttta ttaaagaaac gtttgatgct atttgtcgtt
    14461 aaaaaaccac ggttattagc aaaagcagtc atcagcatgt caatcgctaa tttggatgca
    14521 aagtcacctt tattatgacc gccaagacca tcacaaacaa caaagagtaa atcacccttt
    14581 tggtttaaaa ggactgcggc acgatcttca ttgtgaagtc taactaagcc acgatcactt
    14641 ttagcagcga atgttccttt aactgttgtt gccacgtttc ttctccactt ttgcccgtaa
    14701 ttgtccgcag gccgcatcta tatcactacc aaactcttta cgtacagttg tttgcactcc
    14761 catgcttttt agataattag caaatttata aacatgatca cgagttgatg gttcataatc
    14821 aaattccgca attgggttat acgcgattaa attaacaaaa ccatttgtgc ctttaactag
    14881 gttaactaag gctcttgcgt cttccaaagt atcattaaaa tcattaagca taatatattc
    14941 ataagtcacg cgcttattag ttttactttc atagtatttc acggcagaaa gtacttcttt
    15001 taaaggataa gcattgttaa ttggcataat ttgattccgc ttttcatcag tagccgcatg
    15061 taaagaaatg gctaacttta cttgtaattt taaatcggcg aattctttaa ttttagggac
    15121 taaacccacc gttgaaactg taatatgacg agcaccaatc gcaagtgatt ttgggttatt
    15181 taataagtca ataaaatcta aaatattttc aaagttatca aacggctccc cagtccccat
    15241 aagtacgaca tgcgttgtaa gttttaattc accacgacct tttagatatt cattaatcat
    15301 taatacttga ccaagcattt caccaggtgt taagtcgcgc tttttcttta aaagccctga
    15361 cgcacaaaag cgacacccca tattgcatcc gacttgcgtt gaaacacaga cgctattgcc
    15421 ataacgatag cgcatcaaaa ctgtttcgac gagtgatcca tcaccaagct ttaaaagaag
    15481 tttaacagta ccgtctttac tttcttgtag ttcatgaact gttggtaatg caaaagtaaa
    15541 attattaatc ataaattcgc gtgtaactaa cgctaaatta ctcatattat taaaatctaa
    15601 ttcgtctttt aaatagaccc actcaaacat ttgagttgca ctatattttt taaacccctc
    15661 atttaataaa aagtcttgga gtttaggtaa agaatagtca taaaaattaa gcttcttcga
    15721 catgggcacc tttctttaaa agggcataat agagcgtttc attattaggt ataaagtgga
    15781 aatgttgttt ttcaagtatt aattcaaact ctggataatc acgcaaaaaa cccactgctt
    15841 gcaagtgagt ttcaaaccgc aatacggtag tcgtgataaa cattaaatgt ccatcttcat
    15901 ttacataatc ttttagctct tctaactctt cccggccctt tgcagcaaag aattgtaagt
    15961 tgtctggtaa ccggaagtaa aaatcacgat ggcgaatatc accattaata tttgaacttt
    16021 gcagtgttgt gagcacaaag tcataagttt gggtaatttt atctacataa gtaacatttt
    16081 ctaatgaaaa cttccgtact aaatggcgca cgatatattt tgagcgatta tcatctttat
    16141 aaaaatgaat ttgaatttca ggatatttaa gcgcaagtac aagcattaag gatgtatctt
    16201 cgtgttgcac aaataacaaa tttttaggtt ttttgctttc gataatatcg acaacatcta
    16261 ataatgtttg actagcaatt acaatttcat tgttattgta cgctggtgtt ttcttgatat
    16321 actctttccc tttatattca tattcaccag gttttaagcc acgaataaaa agtggcatat
    16381 tagcaaagaa atcttcatca ctaattacat tttgattaat aagaccatat gtttcacgat
    16441 atctatttgt aaggaattta aggaggtttt tccgtcctat atctgcaatt aattgttcgg
    16501 taagtgctaa tggtacacta aagcgagcgg ctaaagaaag cgctgggttt tcactatgaa
    16561 aatgttttgg aattcgaatt ggaaaacttt catcaggata ttcttgttca aatttaactt
    16621 gtatatcagt aggaagcgca gcaagaaact cttcataaga taaaggcaca acttttttct
    16681 cacgattaaa actaaagaga cttaatgcta aaacttcaaa ttgagttaga gttgtttctt
    16741 cgccttcagt taaagaatac aaataacgga agtatctgtt taaaagagca ttaactaaag
    16801 tacgaaactg atgcttctca ctttgtgagt aactattatc ttgctttaag tccttactaa
    16861 attgttgata agggagactc ccatcaatga ttttttctgt tgtgtctaaa atgtgtttta
    16921 tcatatgtac ctacctatat tagtatatca ctctttttaa aggtgtttct aattaattag
    16981 aaaattagtc gtctttgtct tcgtctgtta gatgacatac ttatctttac aaataaagat
    17041 taggaaactt ttaaatttt
//
LOCUS       DAOSLP010000019        16009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_3819_length_16009_cov_10.091325, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000019 DAOSLP010000000
VERSION     DAOSLP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 16009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16009
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3819_length_16009_cov_10.091325"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..387
                     /locus_tag="PK460_02780"
     CDS             199..387
                     /locus_tag="PK460_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54054.1"
                     /translation="MDKMTFIDRTLCKLLFKMLAKKEKDKLEVWEKALLAAGNRVVDW
                     TNERYLAPITKYLANLNQ"
     gene            587..1768
                     /gene="metK"
                     /locus_tag="PK460_02785"
     CDS             587..1768
                     /gene="metK"
                     /locus_tag="PK460_02785"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010288382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOE54055.1"
                     /translation="MANYRLFTSESVSEGHPDKVCDQISDAILDAVLAINPNARVACE
                     VFATTNFVLISGETSLTKEEEPDYENVAREVIRDIGYTNPKLGFSADAAEIVIKMNQQ
                     SSDIAQGVTQIKPEEQGAGDQGIMFGYATKESAGLMPLAIVIAHKLMRVATKMRKDGE
                     FPGARPDMKSQVTMRYYEDGSSAIDTVLMSVQHDEDVNLEEFKKFIHQKIMIPVVESF
                     GKNTDFKYLVNPTGRFVIGGPAGDTGLTGRKIIVDTYGGYAKHGGGAFSGKDPSKVDR
                     SAAYYARYIAKNLVESDVCDEVEIQLSYAIGVAEPLSVAIDTFGTGKVADEVILAAIS
                     KYFDCRPGMITQEFSLRKPKFKYRDLAALGHFGRPDINIPWEETNKAQALKSYFKKYL
                     V"
     gene            1853..2413
                     /gene="raiA"
                     /locus_tag="PK460_02790"
     CDS             1853..2413
                     /gene="raiA"
                     /locus_tag="PK460_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOE54056.1"
                     /translation="MKYQIVGKNIVVTDAIRNALEKKLSRMNKYFVIDNDIDCRAVVS
                     KIRSTAKVEVTIFTKHMIFRAEESDADLYAAFDKAIDSLEGQMRKLKTRLSRRNRVGL
                     GEAIAQAIAFDNIEDATVNEAENKVVRLKSIYLEPMSLEDAIMRMEALSHKFFLYLDA
                     EDNLISLLYIRNDGGYGIIQAENKVK"
     gene            2512..3348
                     /locus_tag="PK460_02795"
     CDS             2512..3348
                     /locus_tag="PK460_02795"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE54057.1"
                     /translation="MKKNYKLLATDLDGTLFYPKRPRSLISRKNKKFLKKFMDEGNKV
                     VLVTGRSPAYTKNLFEVLGQEVDVIGMNGAYTIVDGQIRDEHFLDFPIEKMLYDLNAL
                     FPILGTMLISQKYPLLISTPPIGKFIQFFYNVYYFLQGRYAEDYHFSNEEFIAEIESK
                     HVYKLMIFFGMSRSGVKRAARANQYLREHYPHQFEASWTGGFIEITPVGVSKANGIKK
                     YAAEKNIPEENINVVGDSGNDISMFKAFYEHSFCMKHSHKNVKKYAKTIIRRFHHLEK
                     YI"
     gene            3362..4219
                     /locus_tag="PK460_02800"
     CDS             3362..4219
                     /locus_tag="PK460_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54058.1"
                     /translation="MNQVTQYILGIYQINMIIRDTVSYVAPRKEQSFSKEIYEHRGRS
                     LELLTAEGSPFAHFVSINKEKADKLVENINEFKKEMYSPESRVFRVVGEGIEVDDKMH
                     HRVYEMAIGIYQTLLDVLGGYIKYAKDNDQLELRIEELVAADEYFFRSLSYFALINDI
                     FKLFKEFSDAMQQHKGEPNPIAKFINEDINKMVQLIAFMNKHNRVTNLTYKKMTDLTN
                     AFIEHMGGQRQLPEGKNFPELFTELNEFALKTLQDAETNWRTIFLPVAKAHQEEMKKN
                     ERKNPEDLS"
     gene            4222..5082
                     /locus_tag="PK460_02805"
     CDS             4222..5082
                     /locus_tag="PK460_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOE54059.1"
                     /translation="MRKTALIFDSTAIIDEEIVKKYDITFVSMNVAIDGVNHRALDID
                     DEEFRKQFHNLKNLKSGSPSILDFEEAINAKFDEGYKEVIVLPLASGLSATFNIAVMA
                     REGLSPERKAATYIHESNNASIGMDLLMHSLVELLDTDPTADELLAALEARAPQNRVL
                     FEIDNLRHLFRGGRLNPLKYIIGTALKIKPLVEFKDGELKVIAQNRSRRKTMDVILGK
                     IDDFVQNFKNVYVGLFTYDKEDNIFKAIHDIIKERWPHIRINFSKKICPIFTTHVGVD
                     GYSVAVVSYN"
     gene            5092..5418
                     /locus_tag="PK460_02810"
     CDS             5092..5418
                     /locus_tag="PK460_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54060.1"
                     /translation="MAKRKIDGGELALYIIYGVIALGGLTLVVLHLIGMNLANLENAL
                     RVADETFAEKMKMDFLVFGSLLVVLAGALSAITLAIYGNRAELEEEKRARRRQRMALE
                     DFSDLE"
     gene            complement(5447..6322)
                     /locus_tag="PK460_02815"
     CDS             complement(5447..6322)
                     /locus_tag="PK460_02815"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HOE54061.1"
                     /translation="MHKDKIINAFKIYLNKDEVNLEYQERFLGGMSNYTYHVKVNGKD
                     YVIRIANKEGKVFVDYATEKLHLFLLEPFNITSKTLFYDTDTGIKISEYIPGDNLTAT
                     LSAEDYAGVARALHKLHALNIEGVDYGQIDRLARYESLVEKHLTPEYFELKAFWMNEN
                     STTYKDADIVFTHGDAQRTNIVKNGDSYTLVDFEFAGMNDRYFDIASFGNINFSDSLN
                     LLDYYLGRKALPHEVRRVKFHRLFQVLQWHVVALQKHLNGQSEKLLIDFKKYSDNYLA
                     FAAYLATKIKALPIK"
     gene            6371..6446
                     /locus_tag="PK460_02820"
     tRNA            6371..6446
                     /locus_tag="PK460_02820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6404..6406,aa:Arg,seq:cct)
     gene            6455..6808
                     /locus_tag="PK460_02825"
     CDS             6455..6808
                     /locus_tag="PK460_02825"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOE54062.1"
                     /translation="MKRHEFTLKPIPFMRIKNKQKVIEMRLYDEKRQKLRVGDFIYFT
                     NTETKEILKVEILELTRYLNFTELYAAYTPKELGYLDGEEVAPSDMEEYYKKDDIDRF
                     GVLAIYINVLNSEID"
     gene            6847..6922
                     /locus_tag="PK460_02830"
     tRNA            6847..6922
                     /locus_tag="PK460_02830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6880..6882,aa:Ala,seq:ggc)
     gene            7010..8293
                     /locus_tag="PK460_02835"
     CDS             7010..8293
                     /locus_tag="PK460_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE54063.1"
                     /translation="MKRKTYLRNQTIYQIYARNHTAEGTFVALIKDLPRIKELGVDIL
                     YLLPIHPIGEIARKGELGSPYAIRDYYLINPEHGTLEDFQTFITLAHSYGFKVMIDIV
                     LHHTSPDAELLAEHPEFYYYKNGKLGNKVGDWSDIIDLDYNNRDLWDYMVEMLKYWAS
                     LGVDGYRADVAPLVPLAFWEYARQELDEDYPELIWLSESVEPHFITNLTAAGHVAHHD
                     KEMYSAFDILYDYDVYNDLKAYLEGKAPLKPYLDGVRYQEQNYPKGYLKIRTIENHDT
                     VRIASLCKTDQILRNVTAWSFFQNGVGFIYGGQEVKATHRPTLFDIDKIDLNVKDQDY
                     YDFMILLVEMKKHPVFAEISSFNIIGDGADDVIVSTLESRVGAAHGFFNLGLKEKEVK
                     TELKDGSYTNVLTNTDIVVNDGKIKVSEPIIIIEE"
     gene            8323..8407
                     /locus_tag="PK460_02840"
     tRNA            8323..8407
                     /locus_tag="PK460_02840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8357..8359,aa:Leu,seq:cag)
     gene            8457..9062
                     /locus_tag="PK460_02845"
     CDS             8457..9062
                     /locus_tag="PK460_02845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOE54064.1"
                     /translation="MHKVSIRSKLTPHQVFTIPNILSFFRLLLIPLIVFLYVGKELYG
                     WTLVVLIISLVTDIIDGLVARKLNMVTDFGKFIDPVADKLTQLAVLFCLVTRFPLMWI
                     PISIMLLKEILQFALRFVVFRYTGEVHSAEWHGKVATFLIYVMMSLHVIWYGITDVAS
                     TLTITITSLSMLLSCVLYTISSAVIIINIKRRKKDKEQLNN"
     gene            complement(9111..9887)
                     /locus_tag="PK460_02850"
     CDS             complement(9111..9887)
                     /locus_tag="PK460_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE54065.1"
                     /translation="MEEYTLLTNKLFSSLKNKEFNLQFQPQINCNNGQINGVESLLRW
                     TTDEGQRVGPNVFIPMLEQTGLIHEVGLWVIDQSLKQHKALVKKGFAPLRFSVNLSVA
                     QFRRDDFVDNVLKLLKKHKVEPQYLELEITESLLSKNFADTIEKLTRLNDAGLHIAID
                     DFGKGYSSLHRLQLVPFNRIKIDKSVVDNITIEPKKVVIAKTIINLAKSLNANITTEG
                     VETKEQAAFMKDLGTDEIQGYYYYSRPLPPKDLEDFLKQK"
     gene            complement(9968..11440)
                     /locus_tag="PK460_02855"
     CDS             complement(9968..11440)
                     /locus_tag="PK460_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE54066.1"
                     /translation="MRLFQTAALIFGIGAAASFFSGYFLTNGDFIREIILSVSIWFFA
                     IFFLFVPHITKKILIQNILFFITSVVGMTFFIVKDAHMGASTVWAVYIVFLLYSIVLD
                     SKRDTLFFLIFTVFTQIVLWITFPNVTIVLDNAQYAKRLFIIILSYVAVRYVTSEYDS
                     KFQGYQRFSRGQEILEKISTNFIAVNQDNVRAKIDEMFMMAAEILQYDQAYLFRFDDK
                     GERATILNAYVRDSEDNSLLLQLGRSFKISEFPQVEPLAGKHSPIFCDDIANLGTHTQ
                     DESANFFLSRGINSFYALPMFVEEKLSGFFVVEYKEKNELTVVESRLHYLKIIANVLG
                     DARKKIIYEKQLYDFAYIDKSTNLPNRNMLINNLEELLLNRDDADKLVVFNVELDNLR
                     MINDTFGHATGEQVVIKSASILQTLMKDGCVLSRIAEDKFIIVMPIAETIAQVMDCAN
                     EITDAFVNPILPADSIGSLFVTTIVGVATFPEDGRDATPF"
     gene            complement(11453..12211)
                     /locus_tag="PK460_02860"
     CDS             complement(11453..12211)
                     /locus_tag="PK460_02860"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOE54067.1"
                     /translation="MDYNALLPIIFYLCACFYSFFGIYTLATNAKSRTNWQFFFLMIS
                     LTIWSFTYAMAYSVDSAETRIMWKSLGVFGWSLFYAFFLRFAIILTKRNEPSKKPFLQ
                     VLFYLPALITIILFGPFGFLMGMQYEFVPGETGLFQAIINNIGQIWFGLYPSAYTIIS
                     LVILIRWRRTIDRESPLRRLLSIFLISIILPFIIGIFLGVFPRFFGLENLPRLTVAFL
                     IPPAVLLFIALRKFGMVFETKTRRDFSPLDPKTT"
     gene            12510..12722
                     /locus_tag="PK460_02865"
     CDS             12510..12722
                     /locus_tag="PK460_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOE54068.1"
                     /translation="MILPIANTERDYVILEVAGTDKLRQYLASLGIIKGKSLRIMSQG
                     KTGTIILVNETRLALNYDIAKLITIA"
     gene            12737..13024
                     /locus_tag="PK460_02870"
     CDS             12737..13024
                     /locus_tag="PK460_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOE54069.1"
                     /translation="MNEEKIIIKVGDLAKLSIVPIGTSGVIHLVSGQGRLRRRLLDMG
                     LTPGAEVFLRKQAPLGDPLELTLRGYELSIRKSEADLIVIRVTDLDAKGDK"
     gene            13026..15299
                     /locus_tag="PK460_02875"
     CDS             13026..15299
                     /locus_tag="PK460_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOE54070.1"
                     /translation="MKKNIRVALLGNPNSGKTTLFNSLTGSTAYVGNWPGVTVEKKSG
                     TYYFKGKGKDREGIDIIDLPGIYSLSPYTPEEVISRNYILKENPDVVINVIDATNLER
                     NLYMTTQLLEIDVPVVLAVNMTDALAESGKTIDYAKLKEKLKIPVVPISALEEKGLDN
                     LMKVTKVEANKKREAYSVITDKKIEKVTEIYKKYKIANPLFHAIKALEKDEIEESENK
                     EAYADVQSLMAQDKEDYEAVIADLRYQFLTPLCAEISTKPHKSEKEKWTKSDRIDQVL
                     THKIFGMLIMIVALFFVFHFTFSGDLLFLNAFGVNFGEGYEGFLSIMVGGEEYRPFAG
                     LIYTDGGINSIGEFFHVLAGDNETGILGLISLGIKALLLKWNAPEWVVGFFYDGLLNG
                     VTAVLGFVPQIMILYTFFSIMEDSGYMARIAFMLDRIFRKFGVSGRAFIPMIMGFGCA
                     IPAMINTRTLATEKERIKTIRVIPFFTCGAKAEFIAIIAAAIAAAVGVDAGLFTFSMY
                     MLGILVAIVSVIVMNKTTQREKVPPFIMELPTYRTPQLKALTRHVYNKGKHFVIKAFT
                     IILASTIVIWLLANFGWDWQMVDENGTGSILQSLGSFIQPLFTPLGFGVQAGEHGWTL
                     TVASIQGIVAKENVTATAETLASIIDVDGFTGIVEAIGLSNAAVVAFSVFNLLTIPCF
                     AAIATAKGELSNRKVYRWTIAYWFLLSYGLGALTYVSFAYVWTLAITIPVIALLIALL
                     IIYDRHKKKQELLLENE"
     gene            complement(15582..15947)
                     /locus_tag="PK460_02880"
     CDS             complement(15582..15947)
                     /locus_tag="PK460_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOE54071.1"
                     /translation="MGLKPSGEMYLKTIYILTKTVHPVRSIDIAEYMNFSKPSVSRAV
                     GNLRKEKYISVNEDGHIFLTPKGLDAAQNIYDRHKVLTKALRILGVSEETALNDACEI
                     EHIISDESFEALKQYLKNK"
BASE COUNT         5304 a         2607 c         3036 g         5062 t
ORIGIN      
        1 acataaaatc attaaaacac ttaatcttgt taattttaat tattcatcta tcctaaatta
       61 ttaagcgata aaaaagtatt aattttttgt gttggtgctt ctccgtttga tgaaaaagct
      121 tttcaaacac ttaaagacca taatttaaaa gatgacctta aacatatacc gtgtttctat
      181 ttgcgcggag cgtggaacat ggataagatg acattcattg atcgcacctt atgtaaatta
      241 ttatttaaaa tgctcgctaa aaaagaaaaa gacaaacttg aagtatggga aaaagctttg
      301 cttgctgcgg gtaatagggt agtggactgg actaatgaac gttatttagc gccaatcact
      361 aagtatcttg caaatctaaa tcaataagca ttcgtattat tttatttcaa ctatatattt
      421 tgttatactt actttaacct tatcaagaac tacattgtgg cattgatgta gtagcaactc
      481 ttatgaacat aagtaagtgc tcgctaaagg caaatcttgt aacgataagg aaagtgttaa
      541 attactttcc ttgcgaaagt aattttaatt aacggaggat aaaattatgg ctaattatcg
      601 tttattcact agtgaatcag taagtgaggg acatcccgat aaagtttgtg accaaataag
      661 tgatgcaatt cttgatgcag tgttagcgat taatcctaac gcaagagtag cgtgtgaagt
      721 ttttgctacc actaattttg tcttaatcag tggtgaaacc tcactaacaa aagaagaaga
      781 accagattat gaaaatgttg ctcgtgaagt aattagagat attggctaca caaatcctaa
      841 gttaggtttt agcgctgatg cggcggagat tgtgattaaa atgaatcaac aaagtagtga
      901 tattgcccaa ggggtaacac aaattaaacc ggaagaacaa ggcgctggtg accaagggat
      961 tatgtttggt tacgctacaa aagaaagtgc agggttaatg cctttagcaa ttgttattgc
     1021 tcataaactt atgcgtgtcg cgactaagat gcgaaaagac ggtgagttcc ccggtgcaag
     1081 acctgatatg aaatcgcaag taacgatgcg ctattatgaa gatgggagct ctgcaataga
     1141 tacagtatta atgagtgtgc aacatgatga agacgttaat cttgaagagt ttaaaaagtt
     1201 tattcatcaa aagattatga tcccagtagt ggaaagtttt ggcaaaaata ctgactttaa
     1261 atatttagtt aacccaacgg gacgctttgt cattggtggt cctgctggtg atactggttt
     1321 aactggtcgc aagattattg tcgacactta cgggggatac gcgaagcatg gtggcggtgc
     1381 ttttagcggt aaagacccga gtaaagttga ccgcagcgct gcctattacg cgcgttatat
     1441 tgctaaaaac ttagtggaaa gcgatgtgtg tgacgaagtc gaaattcagc taagttacgc
     1501 aatcggagta gcggagccac taagtgttgc tattgataca tttggtactg gtaaagtcgc
     1561 ggatgaagta attttagcgg caatttccaa gtattttgac tgtcgtccag gaatgataac
     1621 tcaggaattt agcttacgaa agccgaagtt taagtatcgc gaccttgcgg cgcttggtca
     1681 ctttggtcgt cccgacatta atattccgtg ggaagaaact aataaagcac aagcattaaa
     1741 atcatacttt aaaaaatatt tagtctaaat taaaacttcc tctttataaa ttactattct
     1801 catgttaaaa taagaataca taagccatct cttgatagaa aggaaacgat aaatgaaata
     1861 tcaaattgtt ggcaaaaaca tcgtcgtcac cgacgcaata agaaatgcgt tagaaaagaa
     1921 actttcacgc atgaacaaat actttgtcat cgacaatgac attgactgtc gcgcagtagt
     1981 gtcgaaaatt cgctcgacag cgaaggttga agtaacaatc ttcactaagc atatgatctt
     2041 ccgcgcagaa gaaagcgatg cagatctcta tgccgccttt gataaggcaa ttgattcatt
     2101 agaagggcaa atgcggaaac ttaaaacgag gcttagtcga cgcaatcgcg ttggtttagg
     2161 ggaagcaatt gctcaagcta ttgcctttga taatatcgaa gacgcaactg ttaatgaagc
     2221 tgaaaacaaa gtcgtacggc ttaaatcaat ctatttagaa ccaatgtcac ttgaagatgc
     2281 gattatgcgc atggaagcct tgtcgcataa attcttcctt tatcttgatg ctgaagataa
     2341 tttaatctca ttactttata tccgtaatga tgggggatat ggtattatcc aagcagaaaa
     2401 taaagtaaaa tagttaatag cgacgaagtt tatcaaaaaa atcggctagg atatcctagc
     2461 cttttcttta tttcaataga aataaaaaat atattgtata attattacgc tatgaagaaa
     2521 aattacaaat tattagcaac tgatttagat ggaacactct tctacccaaa gcgaccacgt
     2581 tcgcttattt cacgaaagaa taaaaagttc ttaaagaagt ttatggatga aggcaataaa
     2641 gtcgtattag tcaccggaag aagtccggct tatacgaaaa atctcttcga agtgcttggc
     2701 caagaagtcg atgtgattgg gatgaatggg gcctacacta ttgttgatgg ccaaattcgt
     2761 gatgaacatt ttttagattt tccaattgaa aaaatgctct atgatcttaa tgcgctcttt
     2821 cccattcttg gtacaatgct aatctcacaa aaatatccgc ttttaatctc gactccaccg
     2881 atcggcaagt ttattcaatt cttttacaat gtttattact ttttacaagg tcgctatgcc
     2941 gaagattatc atttttctaa tgaagaattt attgctgaaa ttgaatcaaa acatgtctac
     3001 aaattaatga tcttttttgg aatgtcgcgg agtggtgtta aaagagctgc gcgcgctaat
     3061 caatacttgc gtgaacatta tccgcatcaa tttgaagcaa gttggacagg cggatttatc
     3121 gagattacgc cggtaggagt ttcaaaagct aacgggatta aaaaatatgc agcggagaaa
     3181 aatattcctg aagaaaatat taatgttgtt ggtgattcgg ggaatgatat ttcaatgttt
     3241 aaagcatttt atgagcattc tttctgtatg aaacactcac ataagaatgt taaaaaatat
     3301 gccaaaacaa ttattcgacg gttccatcat ttagaaaaat atatttagaa agaaggtatt
     3361 tatgaatcaa gttacacaat atattttggg tatttaccaa attaatatga ttattcgtga
     3421 tacggtaagt tatgttgcgc cacgcaagga acaatctttt agtaaagaaa tttatgaaca
     3481 ccgcggacgt agtctagaac ttttaacagc agaaggttcc ccttttgcgc actttgtatc
     3541 cattaataaa gaaaaagctg acaaactcgt ggaaaacatt aatgaattta aaaaagaaat
     3601 gtattcacca gagtcacgag tctttagagt agtaggagag ggtattgagg ttgatgataa
     3661 aatgcatcac cgggtttacg aaatggcgat tgggatttat caaacactgc ttgatgtttt
     3721 aggcggatac attaaatacg ctaaagataa tgatcaatta gaattacgaa ttgaagaact
     3781 agtggccgct gatgaatatt tctttagaag tttatcgtac tttgccctta ttaacgatat
     3841 ctttaaatta tttaaagagt ttagtgatgc aatgcaacaa cataagggtg aaccaaatcc
     3901 tattgcaaag tttattaacg aagacattaa taaaatggtg caacttattg cctttatgaa
     3961 taaacataat cgtgttacta acttaactta taagaaaatg accgatttaa caaatgcatt
     4021 tattgaacat atgggtggtc agcggcaact tcctgaaggt aaaaacttcc ctgaattatt
     4081 tactgaatta aacgaatttg cgcttaaaac attacaagat gctgaaacta attggcgcac
     4141 aatcttttta ccagtagcga aagcccacca agaagaaatg aagaaaaatg aaagaaagaa
     4201 tccggaggat ttatcataaa tatgagaaaa acagcactta tttttgattc aacagcgatt
     4261 attgatgaag aaattgttaa aaaatatgac attacttttg tatcgatgaa tgtagcgatt
     4321 gatggtgtca atcatcgcgc actcgatatt gatgatgagg aatttcgtaa acaattccat
     4381 aatttaaaaa atttaaaaag tggttcacca tcaattttag attttgaaga agcgattaac
     4441 gctaaatttg atgaaggata taaagaagta atcgtgcttc cattagcaag cggtttaagc
     4501 gctactttta atattgcagt aatggcgcgt gaaggtttaa gtccagaacg caaagctgct
     4561 acatatatac acgaatcaaa taatgcttca attgggatgg atctattaat gcattcactt
     4621 gtggaacttt tagacacaga ccctactgct gatgaacttt tagcggcgct tgaagctcgc
     4681 gctccacaaa accgcgtgtt atttgaaatt gataatttgc gtcatctttt ccgtggtgga
     4741 aggcttaatc cgttaaaata cattatcggc acagcactta aaatcaaacc gctcgtggag
     4801 tttaaagatg gtgaattaaa agtcattgcg caaaacagaa gtcggcgcaa aacgatggat
     4861 gtaatcttag gaaaaattga tgactttgtg caaaatttta aaaatgttta tgttggatta
     4921 tttacttatg ataaagaaga caatatattt aaagcaatcc atgatataat caaagaacga
     4981 tggccacata ttcgaattaa ctttagtaaa aaaatatgtc ccatcttcac aacccatgtg
     5041 ggagttgatg gttattcagt tgcggttgta agttataatt aggaggaagt tatggcaaaa
     5101 agaaagattg atggtggaga attagcgctt tacattattt atggcgtgat tgcccttggt
     5161 ggtttaactc tagtcgtatt acatttaatt ggaatgaatc ttgctaatct agaaaacgca
     5221 ctgcgtgttg ccgatgaaac attcgcggaa aaaatgaaaa tggattttct cgtctttggt
     5281 tcattacttg ttgtcctagc gggcgcatta agtgcaatta ctttagcaat ctatggtaac
     5341 cgcgcggaat tagaagaaga aaaacgcgct cgtcgtcgtc aacggatggc gcttgaagat
     5401 tttagcgatt tagaataatt actaattatt tatgaaaaaa agagcactac ttaatcggta
     5461 gtgcttttat tttagttgct aagtaagcgg caaatgcgag gtaattatca ctatattttt
     5521 taaaatcaat tagtaatttc tcactttgcc catttaaatg tttttgtagc gctacaacat
     5581 gccactgtaa tacttggaaa agacgatgaa atttaacacg gcgcacttca tgtggtaaag
     5641 cttttcttcc taaatagtaa tcaaggagat taagactatc actaaaatta atattaccaa
     5701 aactagcaat atcaaaatag cgatcgttca ttccggcaaa ttcaaaatcc actagtgtat
     5761 aactatcacc gtttttaaca atgtttgtgc gttgggcatc cccatgcgta aaaacgatgt
     5821 cagcatcttt ataagtcgta gagttttcat tcatccaaaa tgctttaagt tcaaaatatt
     5881 ctggtgttaa gtgtttttct acaagtgact cataacgcgc aagacgatca atttgaccgt
     5941 aatcaacacc ttcaatattt aaggcatgca gtttatgaag ggcacgcgct acaccagcat
     6001 aatcttctgc tgataatgta gcggttaagt tatcgccagg aatatattca gagattttaa
     6061 tcccggtgtc tgtgtcgtaa aatagtgttt tacttgtaat attaaatggc tctagtaaaa
     6121 aaagatgaag tttctcagtt gcgtaatcaa caaacacctt cccttcctta ttagcaatac
     6181 ggataacata atcttttcca ttcactttta cgtgatatgt ataattactc atcccaccga
     6241 ggaatcgttc ttgatattcg agatttactt cgtctttatt aagatatatc ttaaaagcat
     6301 taataatctt gtctttatgc atggatatat catactactt tttaattaaa tttgttatga
     6361 ttaagtatgc gcgctggtag ctcagtggat agagcaatcg cctcctaagc ggtaggtcgg
     6421 aggtttgaat cctccccagt gcgccagcct ttttatgaaa agacatgaat ttactttaaa
     6481 accaattcca tttatgcgca ttaaaaataa acaaaaagtt atagaaatgc gcctttatga
     6541 tgaaaagcgc caaaagcttc gcgtaggcga ctttatttat tttactaata cagagacgaa
     6601 agaaatttta aaagtagaaa tattggaatt aactcgctat ctaaatttca ctgaacttta
     6661 cgcagcttat actcccaaag aattaggtta tctcgatggt gaagaagttg cgcctagtga
     6721 tatggaagag tattataaaa aggacgatat tgaccggttt ggcgttcttg ctatttatat
     6781 aaatgtcctt aatagtgaaa ttgattgaca acttatgcaa atagtattat tataaacaag
     6841 caattagggg ccgtagctca cctgggagag cgcctccatg gcatggagga ggtagcgagt
     6901 tcgatcctcg tcggctccac cattattgta aaaatctcga tatttagatc cccctctaga
     6961 tatcgagttt ttttattact taaagctgtt tatttgctaa aatgaaagta tgaaaagaaa
     7021 aacatattta cgcaaccaaa caatttatca aatttatgcg cgtaatcaca ccgcagaagg
     7081 aacatttgtc gctttaatta aggacctacc aagaataaag gagcttggtg tcgacatttt
     7141 atacttgctg ccaattcacc ctattggtga aattgctcgt aaaggtgagc taggaagtcc
     7201 ttatgcgatt cgtgattact atttaattaa tccggaacac ggtacacttg aagactttca
     7261 aactttcatt actttagcgc attcatacgg ttttaaagta atgattgata tcgtgcttca
     7321 tcacactagt cctgatgccg agttattagc ggagcatcct gaattttatt actataaaaa
     7381 cggcaaactt ggcaacaaag ttggcgactg gagtgatatt attgacttag actataataa
     7441 tcgtgactta tgggattata tggtagaaat gctcaaatac tgggctagtt taggagtaga
     7501 tggttatcgc gctgatgtgg cgccactcgt tccccttgca ttttgggaat atgcacggca
     7561 ggaactagat gaagactatc ctgaacttat ttggcttagt gaatcagtgg aaccgcattt
     7621 tattacaaat ttaacggcag caggtcatgt tgcgcatcac gataaagaaa tgtatagtgc
     7681 ttttgatatt ctttatgact atgatgtcta taatgactta aaagcctatt tagaaggtaa
     7741 ggcaccactt aaaccttatc ttgatggcgt tcgttatcaa gagcaaaact atccaaaagg
     7801 ttatttaaaa attagaacga ttgaaaatca tgataccgtg cggattgctt ctttatgtaa
     7861 aacagatcaa attttgcgta atgtcactgc ttggtcattt ttccaaaatg gtgtagggtt
     7921 tatttatggt ggtcaagaag ttaaggctac tcatcgcccg acactatttg acattgataa
     7981 aattgattta aatgttaaag accaagacta ttatgacttt atgattttac ttgttgaaat
     8041 gaagaaacac ccagtttttg ccgagatttc ttcctttaat attattggcg acggtgcaga
     8101 tgatgtaatt gtttcaacgc ttgagtcaag agttggagcg gcacacggtt tctttaattt
     8161 agggcttaaa gaaaaagaag ttaaaacaga gttaaaagac ggaagttaca ctaatgtgct
     8221 gacgaacact gatatcgttg ttaacgacgg taaaataaaa gttagtgaac ctattatcat
     8281 tattgaagaa taaatttttt gcagtgttaa aataaagtgc aggcagtcat ggcggaattg
     8341 gtagacgcgc aagtttcaga cgcttgtatc gcaagatgtg ggggttcaag tccctttggc
     8401 tgcaccaatt ttaattattt taattagtgt aaatataaag gaaataggag aaaactatgc
     8461 acaaagttag tattaggagt aaattaacac ctcatcaagt ttttaccatt cctaatatct
     8521 tatccttttt ccgcttacta cttattccgc ttatcgtctt tctctatgtt ggtaaagaac
     8581 tttatggctg gacgcttgtt gtacttatca tttcactagt cactgatatt attgatggtc
     8641 tagttgcccg taaattaaat atggtgactg attttgggaa attcatcgac cctgttgccg
     8701 ataaattaac ccaattagcg gttctatttt gtttagtgac tagattcccg ctgatgtgga
     8761 ttccaattag cataatgtta ttaaaggaaa tacttcaatt cgctcttcgc tttgtagtgt
     8821 ttagatatac tggtgaagtt catagcgctg agtggcatgg caaagtagcg acatttctta
     8881 tctatgtaat gatgtcatta catgttattt ggtatggaat aacagatgta gcgtcaactt
     8941 taacaataac aattacttca ctatcaatgc ttttatcatg cgtgctttat acaatttcta
     9001 gtgccgtaat cattattaat attaaaagaa gaaaaaaaga taaagagcaa cttaataatt
     9061 aataagcaat aattaattag tgcattatat ataaaattaa aagtaagtga ttatttttgt
     9121 tttaaaaagt cttcaagatc tttaggtgga agtgggcgtg agtagtagta gtagccttga
     9181 atttcatcag tgcctaaatc tttcataaac gctgcctgtt ctttggtttc aacaccttca
     9241 gttgtaatat tagcgtttaa tgatttcgct aaattaataa ttgttttagc aataacaact
     9301 ttcttcggtt cgattgtaat attatcaacg acgcttttat caattttaat tcggttaaat
     9361 gggacaagtt gtaagcggtg tagtgaagaa tatcctttac caaaatcgtc aatggcaatg
     9421 tgtaatcccg catcattaag tcttgttaat ttttcaatag tgtcggcaaa atttttcgag
     9481 agcaaacttt cagtaatttc tagttcaaga tactgaggtt ccaccttatg ttttttaagt
     9541 aatttcaata cattatccac aaagtcatca cggcggaatt gcgctacaga gagattaacg
     9601 gaaaagcgca gtggggcaaa tccctttttc actagtgcct tatgctgttt aagtgattgg
     9661 tcaatgaccc ataacccgac ttcgtggatt aagccagttt gttcaagcat tgggataaaa
     9721 acatttggtc ccactctttg cccttcgtca gtcgtccatc taagtaatga ctcaacacca
     9781 tttatttgtc cgttattaca attaatttgt ggttggaatt gtaaattaaa ctctttattt
     9841 tttaaagaac taaaaagttt attagttagt agtgtatatt cttcaatatg tgttttaagt
     9901 tcttcggtac taaagactac tttactatcg gatattttcg cttcataggc agcaatatcg
     9961 gcattttcta aaagggtgta gcatctctcc catcttcagg gaatgtggca acaccaacaa
    10021 ttgttgtcac aaatagcgag ccaatactat ctgctggtaa tattgggttt acaaaagcat
    10081 ccgtaatctc gttagcgcaa tccattactt gggcgatagt ttcggcaatt ggcataacga
    10141 taataaattt atcttcggca atacgcgaaa gcacgcaccc atctttcata agcgtttgaa
    10201 gaattgatgc ggatttaatt acaacttgct cacctgttgc gtgtccgaat gtatcattaa
    10261 tcatccgtag attatcaagt tccacattaa agacgactaa cttatcagcg tcatcgcgat
    10321 taagtaatag ctcttctaaa ttattaataa gcatattgcg gtttggtaaa ttagttgatt
    10381 tatcaatata cgcaaaatca tatagttgtt tttcataaat tattttcttg cgtgcatcac
    10441 ctaatacatt agcaataatt tttaaatagt gtaaacgact ttcgacaacg gttagttcgt
    10501 ttttttcttt atattcaaca acaaagaaac cacttagttt ttcttcaacg aacattggta
    10561 aagcatagaa agaattaatg cctctagaca ggaagaaatt ggcactttca tcttgtgtat
    10621 gagtacctaa atttgcaata tcatcacaga agattggtga atgtttacct gcgagcggtt
    10681 ctacttgtgg aaactcgctg attttaaaag aacgtcctaa ctgcagaaga agtgaattgt
    10741 cctcactatc tcttacatat gcattaagaa ttgttgctct ttcgcctttg tcatcaaatc
    10801 gaaacaaata agcttgatca tactgtaata tttcggcggc catcataaac atttcatcaa
    10861 ttttagctcg gacattgtct tgattaacag cgatgaaatt tgtggaaatt ttttcaagaa
    10921 tttcttgtcc cctcgagaat ctttgataac cttgaaactt tgaatcatat tcactagtaa
    10981 cataacgcac agccacgtat gaaagtatga taataaatag ccgcttagca tactgcgcat
    11041 tatcaaggac aatagtaaca ttaggaaatg taatccataa cacaatctgt gtgaaaactg
    11101 taaaaattaa gaagaataat gtgtcgcgtt tactatcaag aacaattgaa tatagtaaga
    11161 agacaatata aactgcccac actgttgatg cccccatatg cgcatcttta acgatgaaga
    11221 aggtcatacc aactacacta gtgatgaaaa agagaatatt ttgaataaga atcttcttcg
    11281 taatatgtgg cacaaataga aagaatatag caaagaacca aatagaaaca gatagaataa
    11341 tttccctaat gaaatcgcca tttgttaaga aataaccgga gaaaaatgat gcagcggcac
    11401 caatcccgaa aattagcgct gctgtttgga ataagcgcaa gcggctttct ttttatgttg
    11461 tcttgggatc aaggggtgag aagtcacgcc ttgtttttgt ttcgaatacc attccaaatt
    11521 tacgcagtgc gataaataat aaaaccgcag gcggaatcag aaatgcaaca gtgagccgtg
    11581 gtaagttttc taaaccgaaa aagcgcggga atacaccaag gaatatgccg ataataaagg
    11641 gtaatatgat tgaaattaag aagattgata aaagccgtct aagcggagat tcacgatcaa
    11701 ttgtcctgcg ccatctaata agtatgacta gagaaataat tgtatatgct gaagggtata
    11761 atccaaacca aatttgacca atgttattaa taattgcttg aaataatcct gtttcacctg
    11821 gcacgaattc atattgcata cccattaaaa agccaaaagg accaaataag attattgtta
    11881 ttagtgcagg taaataaaat aaaacttgta agaagggctt tttacttggt tcatttcttt
    11941 tcgttagaat tatggcgaag cggaggaaaa aagcataaaa aaggctccac ccaaaaacac
    12001 caagtgactt ccacattatt cttgtttcag cactatcaac agagtaggcc attgcatatg
    12061 taaatgacca aattgttaac gaaatcatta ggaagaaaaa ctgccaattc gtgcgacttt
    12121 tcgcattagt ggccaaagta tatatgccaa aaaaactata gaaacaggcg caaagataaa
    12181 agattatcgg aagcaatgca ttataatcca tagttttttg ctgttcctcc tactattaat
    12241 caattatttg attaacacaa gcaaatccta acacatagtt agaaaaaaag cacaacaata
    12301 ttaaaaaaat gcagaaatta tgaatatatt ttacaattgg tgctcttttt aactgtttat
    12361 aaaacgactc tatcacatta ataaactcta taacaataaa tgtttcgatg taaatttatg
    12421 aatatttttt aaaatttaca atattgcttg aaataccggt tagcaacggc taacatttcc
    12481 ttgttagcaa tggctaacta ggggatttta tgatattacc aatagcaaat acggaacgcg
    12541 attatgtcat tttagaagta gcgggcacgg ataaactccg acaatattta gcgtccctag
    12601 gaattattaa aggtaaatca cttaggatta tgtcacaagg taaaacaggc acgattattt
    12661 tagtaaatga aacacgtctt gcccttaatt atgacattgc caagctaata actatcgcat
    12721 aaggaatggt agcaatatga atgaagaaaa aataattatt aaagttggag atttagcaaa
    12781 actaagtatt gttccaattg gtacaagcgg agtaatccac ttagtaagtg gccaaggacg
    12841 gttacggcgt cggcttctag atatgggttt aacaccaggc gctgaagtgt ttttgcgtaa
    12901 acaagcgcca cttggtgatc ctttagaact tacattacgc ggttatgaat tatctattcg
    12961 taaaagtgaa gccgatttaa ttgtaattag agttacagat ttagatgcga aaggtgataa
    13021 ataagatgaa gaaaaatatt agagttgcct tgttaggtaa tccaaattct ggaaaaacaa
    13081 cactttttaa tagtttgact ggttcaacgg cgtatgttgg taattggcca ggtgttactg
    13141 ttgaaaagaa gagcggaact tattatttta aaggcaaagg taaagataga gaaggcattg
    13201 acattattga cttaccaggt atttattctt tatctcctta cacccctgaa gaagttattt
    13261 cacgaaatta tatattaaaa gaaaatcctg atgttgttat taatgtaatt gacgcaacta
    13321 acttagagcg taatttatat atgacaacac aacttttaga aatcgatgtt cctgttgttc
    13381 ttgctgtgaa tatgactgat gctctagcgg aaagtggcaa aacaattgac tatgcaaaac
    13441 ttaaagaaaa attaaaaatc cctgtagtgc cgatttctgc attagaagaa aaagggctag
    13501 ataatttaat gaaagtaaca aaagttgaag cgaataagaa acgtgaagct tatagtgtta
    13561 ttactgataa aaaaattgaa aaagttaccg aaatttataa aaaatataaa attgctaatc
    13621 cgttatttca cgcgattaag gcactagaaa aagatgagat tgaagaatct gaaaataaag
    13681 aagcttacgc tgatgttcaa tcattaatgg cacaagataa agaagactat gaagctgtaa
    13741 ttgctgactt acgttatcag tttttaaccc cattatgcgc ggaaataagt acaaaaccgc
    13801 ataaaagtga aaaagaaaaa tggacaaaat ctgaccggat tgaccaagtg ttaacgcata
    13861 aaatattcgg aatgctaatc atgattgtcg ccttattttt tgtatttcat tttactttca
    13921 gtggagacct actattttta aatgcttttg gtgttaattt tggtgaaggt tatgaaggtt
    13981 tcttaagtat tatggtaggt ggcgaagaat atcgaccatt cgccggcctt atttacactg
    14041 atggtgggat taattctatt ggtgaatttt tccatgtact agcaggtgat aatgaaactg
    14101 gaatactggg attgatatct ttaggaatta aagcactatt acttaaatgg aatgctcctg
    14161 aatgggtcgt tggttttttc tatgacgggc tattaaacgg tgttactgct gttttagggt
    14221 ttgtcccaca aattatgatt ctttacactt tcttttcgat tatggaagat agtggatata
    14281 tggctcgtat tgcctttatg ttagatcgta ttttccgtaa atttggtgtt tctggtcgtg
    14341 cttttattcc aatgattatg ggctttggct gtgcaattcc tgcaatgatt aatacacgca
    14401 cactagcgac tgaaaaagaa agaattaaaa caattcgcgt tattccgttt ttcacttgtg
    14461 gcgcaaaagc tgaatttatt gccattattg ccgcagcgat agcagcagca gttggtgttg
    14521 atgctgggct ctttactttt tccatgtata tgcttggtat tttagtagcg attgtttcag
    14581 ttatagtaat gaataaaaca acgcaaagag aaaaagtacc accctttatt atggaattac
    14641 caacatatcg tacgccccaa ttaaaagcac tcacgcgtca tgtttataac aaaggtaaac
    14701 actttgtcat caaagcattt actatcattt tagcgtcgac gatcgtgatt tggcttttag
    14761 caaactttgg ttgggattgg caaatggtcg atgaaaatgg tacaggctca attttacaaa
    14821 gtcttggttc atttattcag cccttattta caccgcttgg ttttggtgta caagctggtg
    14881 aacacggctg gacattaacg gtcgcttcaa tccaaggtat agtggccaaa gaaaatgtta
    14941 ccgcaacagc ggaaacacta gcgagtatta ttgatgtaga tgggtttacc ggtattgtcg
    15001 aagcgattgg actaagtaac gctgctgttg tggcctttag cgtgttcaac ttactaacta
    15061 ttccttgttt tgcggctatt gctacagcta aaggtgaatt aagtaatcgc aaagtatatc
    15121 ggtggacaat tgcttattgg ttccttttaa gttatgggtt aggcgcatta acttatgtta
    15181 gtttcgctta tgtgtggaca ctggcgataa cgataccagt tatagcacta ttaattgcct
    15241 tgcttattat ttatgatcgc cataaaaaga aacaagaatt actattagaa aatgaataac
    15301 aaaggaagat taaaattttg cattggacag aaatattcac gattatattt attattgttg
    15361 tactaactag cactattctt tattttgtta aaaaaagaca aaaaggcgaa acaggatgtg
    15421 gggttagttg tggtgggtgt cccacgacaa atattgacat taagtcatat tatcgtaaac
    15481 attataagtc gcttaaataa aacaagaaat gattattaaa aacgggctgt taatgaagat
    15541 tatattcata agcagccctt tatttttatg aagtttaagt attatttatt ttttaggtac
    15601 tgtttaagtg cttcaaagga ttcatcgcta ataatatgtt cgatttcaca agcgtcatta
    15661 agtgccgtct cttcacttac tcctaaaata cgcagcgcct tagttagcac tttatgtctg
    15721 tcatatatat tttgtgcagc atcaagccct ttaggcgtaa ggaaaatgtg accgtcttca
    15781 ttaacgctta tatacttttc tttccgtaaa ttaccgacag cgcgactcac acttggttta
    15841 gaaaaattca tgtattcagc aatatcaatt gaccgaacag gatggactgt cttagttaaa
    15901 atataaattg tttttaaata catctcacca gatggtttta gacccatata accgacttgt
    15961 attatctaat catgccccgg tacttcgatt aggtaataca tctcctttc
//
LOCUS       DAOSLP010000020        15936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_3841_length_15936_cov_7.880864, whole genome shotgun sequence.
ACCESSION   DAOSLP010000020 DAOSLP010000000
VERSION     DAOSLP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3841_length_15936_cov_7.880864"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1115)
                     /locus_tag="PK460_02885"
     CDS             complement(177..1115)
                     /locus_tag="PK460_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54072.1"
                     /translation="MKTRKLKIFALALTLPLLTGCKLIHNFICGIALNVAFNSPDLLS
                     KVNVVEGGAKLTPIEDKPTSADKRASLTYGIEALVLPKRIETVQYGYEIIIDFTFTFS
                     EGAQDYFYIRDVEDAQEGEYNVDGTILYPTGTVENPSNEQEISDWLTVWRMFDLKNQD
                     AKDITITLTGKSLNKTKSQTYYFNLNGDLIALPEQGDVDIDREYALVVEQAGVPKYLD
                     VVVPKEELVVGKNIPLKVMWVEFLLEEGTGTLTIDIETDLSADYDVTLPTENSYAVTV
                     NETLIDYPVNVRLKLGHPLPSEDLTIRFKAVVTSNN"
     gene            1535..1972
                     /locus_tag="PK460_02890"
     CDS             1535..1972
                     /locus_tag="PK460_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOE54073.1"
                     /translation="MSKLHNVLNKQVANLAVFFVKLHHHHWYIKGQHFYGLHAKFEEF
                     YDEVNELYDAVAERLLMIGGKPYSTMKDYLANSSLVEASGGETATEMVTAIKQDFKTL
                     RDEFNALIKVAQDEGDEVTTDLLLGAVTSFDKHIWMLNATLGK"
     gene            2084..2159
                     /locus_tag="PK460_02895"
     tRNA            2084..2159
                     /locus_tag="PK460_02895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2117..2119,aa:Val,seq:gac)
     gene            2277..3416
                     /locus_tag="PK460_02900"
     CDS             2277..3416
                     /locus_tag="PK460_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54074.1"
                     /translation="MIKIVPVYGGRKLRQFINFPVKLYKNSKQFTPYIFEDEISNLTP
                     SKNPASAWCDFKLFLAYRDRKIVGRICGIISHYANKKYDQKRVRFNRIDMIDDIEVTK
                     ALIKAVEEWGYSRGMNEIVGPLGYSDQDKEGLLVEGFEYSNMFATFYHFPYYMKHLEE
                     LGFVSDAVWVEHRIIIPKEIDERFERVSEMVKRKYNVKVRRLKSKRQIQPYVRKALAL
                     TNRAYDHLYGYVPMSEKLMDMLARQYVPLINLKYLILVVDEKDELLAFGLSIPSPVFA
                     LKKIKGKLFPFGFIRFLRALKKSKQLDLLLVAVEPELQNSGLANVIMYESMKSAIADG
                     IEYAETGPQLEYNKPVQRLWKHLNYFNHKRRICYVKPITEVEGEE"
     gene            3406..4674
                     /locus_tag="PK460_02905"
     CDS             3406..4674
                     /locus_tag="PK460_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE54075.1"
                     /translation="MRNRKPKKQRIVKKRHQRYVNLANFLLGRLIRRSYGVKQAKIDI
                     KQYEPCLIVSNHILTFDPILMTSIFGHQIYYVASELIFSLGWKSRLLTHCFAPIPKTK
                     SMADVTTVKNIMQIVKEGGSVGVYVEGNATMTGGLSSMPAAIGKLVLRLKIPLVIFNF
                     EGGYLSLPRWATYTKREKHVRGKIRKVLYYEDYKDMTADEINELIIENININAYEESP
                     QYTYRGKRNAEGLHRLLFTCPICHGVNTAKTAYNNYSCAKCGEILTYDEHGYLHSTKW
                     DKPKNLIELDKANNLAYQEHIINNPNFSVSVPGNFTEVFRKRRKHFGPATLTLSREGL
                     TIKFKRKMKRHVTQHFTIDELDTMAMQQKEVLVIYPRHEQTKMLKIPKTEIVSSYQFV
                     VTLQILKNILQYPDAKPDEVPRLSAQDLGL"
     gene            4708..6207
                     /locus_tag="PK460_02910"
     CDS             4708..6207
                     /locus_tag="PK460_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54076.1"
                     /translation="MRTLFLANTWDFNPFWISIFQIGVVGALILIANTIRRKTPFLRN
                     YLVPTGLIAGFLGLGLKYLFQWLGVEIYGTPIVDESYMHFLTYHSLAIGFIALGLVTA
                     EKKVEKEGRALKSGALIVGGYLIQGIVGLILSLGVSLIFVNYAIGKAPYAGVLLPLGF
                     GEGPGQAGNMGDVYEKLTDYNALAGGRDFGLSIAAMGFVVAAVVGTVILNVVARKGLV
                     TRRKAEVTETFGGLADSQYAVVDHPEEIPVVESVDKFTIQVAFVIAIYLIAFGIMSLV
                     SFLVVDLAKFASIRGIIWGFNFLFAIITTMIVKAVLNLLRKKKIMKRKYINNFMQNRI
                     AGLAFDVMIAAGIMSINFGKLNDPSFWVLLALMSVFGTIVSYYYINFVAKRYFPKTRW
                     YTFFGFFGMLTGTMSEGVALLREIDPYFETGVAEDLVNGSGTAALFGVPMLLITTVVY
                     RGWVGFWVSFALLIVLFAAMILVLHFVTKSRNKKLGAETNGEKEVLETE"
     gene            6351..9038
                     /locus_tag="PK460_02915"
     CDS             6351..9038
                     /locus_tag="PK460_02915"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOE54077.1"
                     /translation="MEQKSIKQTLEQLNSCRENGLTTEEALKRQEVHGKNELPKAKKK
                     TIVGVFFSQMNNAMIYILLVAAVISFVMALIEGDGSFFEPILILAIVLLNAVIGAIQE
                     LKADKALEALEKLSAPTTVVRRDGELKEIKASELVPGDIVIIEDGRTIPADLRLISSA
                     NLKVSEASLTGESVPVEKDANIVYDRPMPIGDRKNVVYMSTPVVYGRGEGVVIGTGLQ
                     TEIGQIAKMLQTTKQEETPLQKRLADLSKLLGIITFVLVALLFIIALIKDPTWDNIKN
                     MFLLSISLAVAAVPEGLPAVVTITLALGVQRMVKVHTITRRLPSVETLGAVSVICSDK
                     TGTLTQNVMTVVSAYTNEKQYEVEDFSADTVGELADGLSLCSDASVDHGVYGDPTEIA
                     LVNFANLIDHHKDDLEAATPRVEELPFDSVRKMMSTLHKTGKTYTQYTKGAMDNVLEL
                     TTHISVNGKVREIKKSDITAINDVASKMAKRALRVLALAKKEVPFAGISEENLVFVGL
                     VGMVDPPRPEAIPAVENLKGAGITTIMITGDHADTAYAIAHELGITDSPSNVLTGAEL
                     DALNEDALKDALLTKRVFARVSPENKVQIVNALQANGEIVAMTGDGVNDAPSLKTADI
                     GIAMGITGTDVAKGAADMVLTDDNFASIEKAVQEGRGIYANIRKTVWFLLSSNFGEVI
                     TMLVAVLVGYPFPLSALHILWVNLITDGVPALALGADSKDPDIMKEKPRDKKESLFAN
                     GGYFITIFFGSVIALITTLAFLFIPVVQHGMRSVQDINTFFAENPELKVRAMTYAFTA
                     LGVSQLFHMFGMSNLNKTFFRTFTKKKWFLWVAFFIGFLLQIAVTEIHALTVAFGTVE
                     LTFIEWVFLALLSTTPLVIHELRVFFRFIREKIKNRKSH"
     gene            9100..11283
                     /locus_tag="PK460_02920"
     CDS             9100..11283
                     /locus_tag="PK460_02920"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOE54078.1"
                     /translation="MKKRNIFTKFSLTAILLSALFAFSSVRASSNIVRTEAWSGTQTS
                     TEGTYYSTVGSETGTALLNKLNTITGNPTPQTSYDWYRYEAADEAENESNKVLLIYSR
                     QKVLKTAHVSGSTGWNREHTYPDSKISGAAKSDNHHIFADDNRTNSTRGNMPFGVVAQ
                     TSTNRVKDGYGNLTDCYKDGTYFYPNEPARGEVARATMYVNMRYGYSVTGNFQSIALM
                     LEWHLQNPVSNREIYRNNTVHTMQKNRNPFIDHPEYACRIWGNTNSETQAICGAAQQV
                     DVTSVSVSPSSGSINLLDVNKTLQLSANVLPANATNKSVNWSTSDSSIATVDANGLVT
                     GKAVGNVTITATSVSNPSVKGTASIAVSNTSIPVTGVTISDKSFTLVKDATKTLTATV
                     SPANASNKNVTWSSSNTSVATVSSSGVVTGVSAGNATITVTTVDGGKTDTANVTVANA
                     PPTQSETTTYTFSTKGWKATPANWQSMYPANGFETDQNYLRGMQVLGNNTAYGNSPYF
                     DNVKKVIFGTAASSSGAGTYSAYLVTSSTSKLKDGTQIGTDVTVSKPGATIIERTFEL
                     TTPASGHVQAIIDATSNSLYLKYVTIEVATPINYQGEADLWANGFLESTSAGCSSQSQ
                     STLSSIWPNLSNEYNALAAEVKAIIEGSTPNSAGSEVEQALARYVFIVNKYGFTAFIN
                     GIPASGVHDTMHNDKQLAEPLLILFSVATLSLIPFVYKRKKRTLN"
     gene            complement(11348..12445)
                     /locus_tag="PK460_02925"
     CDS             complement(11348..12445)
                     /locus_tag="PK460_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54079.1"
                     /translation="MKKQGLAFLLLGTFLLTGCNKPPKMEKVQIMYKYSNLTEVINIV
                     DDENQTALEQLELKITDKENFVLVSYADYTCSCWSVFRDHVLRNYINTKKIPIYVIET
                     DALGNDFKGLPIRKDLTNTPVIGIFAGGVCKYSIDYTSKSEIFIERDKFNEWMDARIK
                     DPLMTYISLEEVNTLLKGTEPFLLNWSYSICPDCVALDKQFMPGYIETLKKAPKMPYY
                     IIESKPIRDAGNWLNVKDIYGLSDKNNTVSGYATGYVPTLQVIRPDGNGATYLANNDI
                     SPMIDDMLVFQNDQVKKVDGVYKIKDSYYNGVRATRYLGEYESEVGKVVDPSIVMETE
                     YNGVLNTYFAPGSRYELHANYATKFFDHYWR"
     gene            12737..14263
                     /locus_tag="PK460_02930"
     CDS             12737..14263
                     /locus_tag="PK460_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE54080.1"
                     /translation="MDKLTKHNVKKEDVIIELKDISKEYDGKTVVDDFNLYVKRGEFI
                     TFLGPSGCGKTTTLRMIAGFELPTTGTITLNGEDITKLPPYKRPVNTVFQRYALFPHL
                     DVYDNIAFGLKLKKVKDTKNGKEVMRKLTFKEIDEKVVRALKMVDLEQFEARDIATLS
                     GGQQQRVAIARAIVNEPEILLLDEPLGALDLKMRKEMQIELKYLHKRLGITFIYVTHD
                     QEEALTMSDTIVVMNDGLIQQIGTPLDIYNEPKNPFVADFIGESNIYNATMAGKLKVK
                     FLGATFDCVDDLPTNEKVDVVIRPEDIKIVAAGKGNVDGKIISRVFKGVHYEYDVQVG
                     RKSEVIIQSTIEYPENEKVGLAVAPDDIHIMKKEVTTNVYADAWIDNKNRLMIGDNAF
                     EIDITQLLKGSTVDEEGYLVGPDAKKYDLDDVDVVATIDFKDIELDDVIEEDAPMGQI
                     ISIIWLGDHYQVIVRTNDEEDFIVDSEYLWNEGDLVSIIIKPEDIKLKLKGELVPYEN
                     "
     gene            14253..15056
                     /locus_tag="PK460_02935"
     CDS             14253..15056
                     /locus_tag="PK460_02935"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE54081.1"
                     /translation="MKIRFKRSYIGIPYVLFMVLFVLLPLFFVAFYAFTNSAGRFSFE
                     PMVNFFTSSAKWRVLFVSFIFGILNTALCLVIGYPLAMILANRKYNTNAVLVMLFVMP
                     MWINFIIRSWAMRDVLVFLGIRGGEYPELATLLGLTYNYLPFTILPLYSTMLKMDKNQ
                     IEASYDLGAGPVTTFFKVIVPMTMPGIISAATMVFMPTMTSYVIADILGEGKVTLFGK
                     YIEIYFTQNIWNEGSFLALVMLALIGISVLVPKLLTRKEAKVKGVGIYD"
     gene            15049..15912
                     /locus_tag="PK460_02940"
     CDS             15049..15912
                     /locus_tag="PK460_02940"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE54082.1"
                     /translation="MIKKILAKVYVYLILFLLYLPILFLIIYSFTPAETTGVWEGFSF
                     ELYGRVFLNEKIMRALLNTLIIALSSATVATILGTLGAIGVFYMKKRPQRIMEGINEI
                     PVVNAEIVVAISLAILFRVFANFLEARGVATNSPNFVTLLIGHVVLSISFVYLNVKPK
                     LQQMDPNDYEAALDLGAKPMYAIRKVVLPAIVPGIISGFMLAFTLSLDDFIITNFLRN
                     QSFDTLSTYVQGVIVRAAIPAEMRALTTYIVLVTLAVVLVSGIKRESRSSRKAMLRAQ
                     KREVLTINKSS"
BASE COUNT         5068 a         2611 c         3171 g         5086 t
ORIGIN      
        1 ctttttgata tatatttact atttaacttg attaagaata gttaatctcc ttgttattat
       61 atgttcatat atattttaaa ttgtgacgac aacataaaat cagcgtattg ttaaaaaaat
      121 cgcaaataat tgaaaaataa ttttaacact taaatttaat tcacttaact aaaactctag
      181 ttattactag taacaaccgc cttaaatcta atggtgagat cttcagaagg aagcggatga
      241 ccaagtttta atctaacatt aacgggataa tcaataagtg tttcatttac tgtaacagcg
      301 tatgaatttt cagtgggtaa tgttacatca taatcagcgc ttaagtctgt ttcaatatca
      361 attgttaatg ttccagtgcc ttcttcaagc aaaaattcaa cccacattac tttaagcgga
      421 atgttttttc cgaccactaa ttcttcttta ggaactacaa catcaagata ttttggaaca
      481 ccagcttgct ccacaactaa ggcatactcg cgatcaatat caacatcacc ttgttccgga
      541 agcgcgatta agtcgccatt taaattaaaa taataagttt gagactttgt tttattaagt
      601 gacttacctg ttaaagttat agtaatatct tttgcgtctt ggttttttaa atcaaacatt
      661 cgccatacgg ttagccaatc acttatttct tgttcgttac tcggattttc aacagtgcca
      721 gttggataga gaatagtacc atcaacatta tattcgcctt cttgtgcgtc ttcgacatca
      781 cgaatataga aataatcttg cgctccttca ctgaaagtaa aagtaaagtc aatgattatt
      841 tcataaccat attgaacagt ttcgattcgt ttaggaagaa cgagtgcttc gataccataa
      901 gttaaactcg cgcgtttatc tgcggaagtt ggtttatctt caattggtgt taactttgca
      961 ccaccttcta caacattaac tttgcttaat aaatctggtg aattaaaagc gacatttaaa
     1021 gcaatgccac aaatgaaatt atggattaac ttacacccag ttagtaaagg tagtgtaagt
     1081 gcgagtgcga atattttaag tttacgagtt ttcataacaa atcctcctgt atccattgta
     1141 ctttaaaaca agaaatcttg ttaggagtat ttatattgat taagtgataa aagagcaaaa
     1201 aaaatcttac acaaggtgtg caagattatt tgttattgct gaagattact tgatcaagca
     1261 gtaacttcgc tagtttattt aagaatttac gcatactttc acctccttaa cgttgctatt
     1321 cttttagggg ggcggcaagt aagttaactt acttacaggc tcattataca ccaattataa
     1381 atataagcaa taaaaacact caaaccccat aaataaaact tataatatgt gataatagcg
     1441 attatatgtt gatatttttt aaagggagca tcattttagg taacattttc aaataatggt
     1501 ataatgtgta ttgaagaaaa aaggaggaaa aattatgtca aaattacata acgtcttaaa
     1561 taaacaagtt gcaaacttag ctgtgttttt tgttaagtta catcaccacc actggtatat
     1621 taaaggtcaa catttttacg gattacatgc taaattcgaa gaattttatg atgaagttaa
     1681 tgaactttac gatgctgtag ccgaacgtct tttaatgatt ggtggtaaac catattcgac
     1741 aatgaaagat tatctcgcta atagttcgct tgttgaagct agtggcggtg aaactgctac
     1801 ggaaatggta acagcaatta aacaagactt taaaacctta agagatgaat ttaatgcact
     1861 aattaaagta gcacaagatg aaggtgatga agtaacaact gacttactac ttggtgctgt
     1921 tacatcattt gataagcaca tatggatgct taacgctaca ttaggtaagt aaattttagc
     1981 gaaacaaccc cagttcaata tatttcaaac tggggttttc ttttttataa atcgcaatta
     2041 tatgaattga caaagttaat cgctttgtta tacttatgtg gctggatgtt tagctcagct
     2101 gggagagcat ctgtttgacg tacagaaggt cgggggttcg attccctcag catccaccat
     2161 ttttttaaaa atagctcacg caatcgtgag ttttttattt tcgttcatta ttcataatat
     2221 gttttacttc aaatataatt tgattatgtt aaaattatca agtagaggaa tgtattatga
     2281 taaaaatagt ccctgtgtac ggcggaagaa aacttagaca attcatcaac ttccccgtca
     2341 aattatataa aaattcaaaa caatttactc cgtatatttt tgaagacgaa atatcaaatt
     2401 taactccaag taaaaatccg gcttcagcgt ggtgcgattt taaactgttt ctagcatata
     2461 gagaccggaa aattgttggt cgcatttgcg gtattattag tcattacgct aataagaagt
     2521 atgatcaaaa acgtgtccga tttaatcgaa tcgatatgat tgatgatatt gaagtaacaa
     2581 aagcactcat taaagcagtg gaagaatggg gttattcacg cggtatgaat gaaattgttg
     2641 gtcctttagg ttatagtgac caagataaag aaggactact tgtggaaggg tttgaatatt
     2701 caaatatgtt tgccacattt tatcacttcc cgtattacat gaaacattta gaggaattag
     2761 gatttgtaag tgatgctgta tgggttgagc accgcattat tattccgaaa gaaatcgatg
     2821 agcgttttga acgcgttagt gaaatggtaa aacgcaaata taatgtcaaa gttagaagac
     2881 ttaaatcaaa acgacaaatt caaccatatg tccgcaaagc tttagcgtta acaaatagag
     2941 catacgacca tctttatggt tatgtaccaa tgagtgaaaa actcatggat atgctagcgc
     3001 gacaatatgt cccgcttatt aacttaaaat atcttatttt agtagtggat gaaaaagatg
     3061 aattattagc gtttgggctt tcaattccgt ctcctgtttt tgcccttaaa aagattaaag
     3121 gaaaattatt cccgtttgga tttattcgct ttttacgcgc acttaagaaa tcaaaacaat
     3181 tagacttact ccttgttgcg gttgaacctg aattgcagaa tagtggtctt gctaatgtaa
     3241 taatgtatga atcaatgaaa agcgcgattg ccgatgggat tgagtatgct gaaactggtc
     3301 cgcaacttga atataataaa cctgtccaac ggctttggaa acatctaaat tactttaatc
     3361 ataaacgccg gatatgttat gtaaaaccta tcacggaggt tgaaggtgag gaatagaaaa
     3421 ccgaagaagc agcggattgt taaaaaacgt catcaacgtt atgtcaatct cgctaacttc
     3481 ttactaggta gattaattcg tcgttcttat ggtgttaaac aagcaaagat tgatattaag
     3541 caatatgagc catgtttaat tgtcagtaat catatcctta cttttgatcc gattttaatg
     3601 acatcaattt ttggtcatca aatatattat gtcgctagtg aattaatctt ttcacttggt
     3661 tggaaatcaa gacttttaac gcactgtttt gcccctattc ctaagactaa aagcatggca
     3721 gatgtaacaa cggttaaaaa cattatgcaa atcgttaaag aaggtggtag tgttggtgtt
     3781 tatgttgaag gaaatgcgac gatgactggg ggactaagta gtatgccagc ggccattgga
     3841 aagttagtgt taagactaaa aattccgctg gtgattttca attttgaagg tggatactta
     3901 tcgcttccac gctgggcaac ttataccaaa agagaaaaac atgtacgcgg taaaattcgc
     3961 aaggttttat attacgaaga ttataaagat atgacggctg atgagattaa tgagctcatc
     4021 attgagaata taaatattaa tgcttatgaa gaaagtccgc aatatactta tcgcggtaaa
     4081 cgcaacgctg aagggttgca tcgtttacta tttacatgcc caatttgtca cggtgtgaac
     4141 actgcaaaaa cggcatataa taattattca tgcgcaaaat gcggggaaat attaacttat
     4201 gatgaacatg gatatttgca tagcacgaag tgggataaac ccaaaaattt aattgaatta
     4261 gataaagcaa ataatttagc gtatcaagaa catatcatta ataatcctaa ttttagtgtt
     4321 agtgtccctg gcaattttac ggaagtcttt agaaagcgcc gtaaacactt tggacctgcc
     4381 acattaacac tttctcgtga aggtttaact ataaaattta aacgtaaaat gaaacgacat
     4441 gttactcagc attttacgat tgacgaatta gatacgatgg cgatgcaaca aaaagaagtt
     4501 ttggttattt acccgcgcca tgaacaaact aaaatgctaa agattccaaa aaccgaaatt
     4561 gttagttcct atcagtttgt agttacctta caaatcttaa aaaatatctt acaatatcct
     4621 gatgcaaaac ctgatgaagt tccgcgtctt tctgcacagg atttaggtct atagtcgtta
     4681 gtgtaaaaat aaaggaggaa aagagatatg cgtacattat ttttagctaa tacgtgggat
     4741 tttaatccgt tttggatttc aatctttcaa atcggtgttg ttggtgcact tattttaatt
     4801 gccaacacta ttagacggaa aacacctttc ttacgtaact acttagtgcc aacaggttta
     4861 atcgctgggt ttcttggttt aggtttaaag tacttattcc aatggcttgg tgtagaaatt
     4921 tatggtactc caattgttga tgaaagttat atgcattttc taacttatca tagtttagca
     4981 attggattta ttgctttagg gttagtaact gcggaaaaga aagttgaaaa agaaggtaga
     5041 gccctaaaaa gcggggcatt aattgttggc ggatatctga tccaaggtat tgttggactt
     5101 atcttgagct tgggtgttag tttaatcttt gttaattacg ctattggcaa agcaccttac
     5161 gctggtgtct tattaccgct tggttttggt gaaggccctg gtcaagctgg taatatgggt
     5221 gatgtttatg aaaaattaac ggattataat gcccttgctg gtggtcgtga ctttggtcta
     5281 agtattgccg caatgggctt tgttgtcgcc gctgttgttg gaacggtaat cttaaatgtc
     5341 gttgctcgca aagggctagt aacacgaaga aaagctgaag ttacagaaac ttttggcggt
     5401 ttagcggata gtcaatatgc ggtggtagat catccagaag aaataccagt tgtggaatca
     5461 gttgataaat ttactatcca agtagcgttt gttattgcta tttatttaat tgcatttggt
     5521 attatgtcac tcgttagttt tcttgttgta gatttagcaa aatttgcgtc tatacgcgga
     5581 attatttggg gatttaactt cctttttgcg attattacga caatgattgt taaagcagta
     5641 ctaaatttat tacgcaagaa aaagattatg aagcgtaaat acattaataa ctttatgcaa
     5701 aatagaattg ctggtttagc ctttgatgtg atgattgccg caggtattat gtcaattaac
     5761 tttggtaaat taaatgatcc aagtttctgg gtgttactcg cgcttatgag tgtatttggc
     5821 acgattgtca gttattacta tattaacttt gttgctaagc gttatttccc aaaaacgaga
     5881 tggtatactt tctttggttt ctttgggatg ttaacgggaa caatgagtga aggcgtcgcc
     5941 ttgctacggg aaattgatcc atattttgaa acaggagtag cagaagactt agttaacggc
     6001 agcggaaccg ctgcgctatt tggtgtaccg atgcttctaa ttactactgt tgtctatcgt
     6061 gggtgggtcg gattctgggt gtcttttgcc cttctaatcg tcttatttgc cgcaatgatt
     6121 ttagtgctgc attttgttac taaatcacgc aataagaaac tcggcgcgga aacgaatggt
     6181 gaaaaagaag tactagaaac agaataaaac aatttaaaac tacaaataag cactgcatgg
     6241 cggtgcttat tttttactaa tttactcaaa tgattacatt tgtagtaaaa tatcaacgta
     6301 cgctattttt ggacttatta gatgaattgt cactagtcct ggaggatcat atggaacaaa
     6361 aatcaattaa acaaacactc gaacaactaa attcgtgtcg cgaaaatggt ttaacgacgg
     6421 aagaagcact taaacgtcaa gaagtgcacg ggaaaaacga attaccaaaa gcgaaaaaga
     6481 aaacaattgt tggtgtcttt ttctcgcaga tgaataatgc gatgatttat atattacttg
     6541 tcgctgctgt tatttctttt gtgatggcgc taattgaagg ggatggttcc ttttttgaac
     6601 ctattttaat tttagcgatt gtattactta atgcagtaat tggagctatt caagagttaa
     6661 aagcggataa agcacttgaa gcactagaaa aacttagtgc ccctacaaca gttgtacgac
     6721 gggatggtga acttaaagaa attaaggcta gtgaacttgt tccaggtgac atcgttatta
     6781 ttgaagatgg tcgtacgata cccgcggact tacgcttaat tagtagcgca aaccttaagg
     6841 ttagcgaagc tagtcttaca ggtgaaagtg ttcctgtgga aaaagacgca aacattgttt
     6901 atgaccgtcc aatgccaatt ggtgatcgta aaaatgttgt ttatatgtcg actcctgttg
     6961 tctatggtcg cggtgaaggg gttgtaattg gtactggttt acaaactgaa atcggtcaaa
     7021 ttgcgaagat gctccaaaca acgaagcaag aagaaacacc attacaaaaa aggcttgctg
     7081 acttatcaaa attattaggg attataactt ttgtattagt cgcgctttta tttattattg
     7141 cccttattaa agatccaact tgggacaata ttaaaaatat gttcctgctt tctatttctt
     7201 tagcagtagc ggcggtccca gaaggcttac cagcggttgt gacaattaca ctagcgttag
     7261 gtgtgcaacg gatggttaaa gttcatacaa ttaccagaag acttccgagc gttgaaacgc
     7321 ttggtgccgt cagtgttatt tgtagtgata aaactggaac tttaacacaa aatgtgatga
     7381 ctgttgttag tgcatacact aatgagaaac agtatgaagt ggaagacttt agtgctgaca
     7441 ctgtcggtga actagccgat ggtcttagtc tttgtagtga cgcaagtgtt gatcacggag
     7501 tttatggcga cccaacggaa attgcgcttg tcaattttgc taatctgatt gaccatcata
     7561 aagatgattt agaagcggca acaccgcgtg ttgaagaatt accatttgat agtgtaagaa
     7621 agatgatgtc aacattacat aaaactggta aaacatatac ccaatacaca aaaggggcaa
     7681 tggataatgt tttagaactt acgacccata ttagtgttaa tggtaaagtt cgtgaaatta
     7741 aaaaaagtga tattacggca attaatgatg tcgctagtaa aatggctaaa cgtgcacttc
     7801 gtgtgttagc tttagcgaag aaagaagtgc cttttgccgg tataagtgaa gaaaaccttg
     7861 tttttgtagg cttggtagga atggttgatc caccgcgtcc agaagcaatt cctgctgtgg
     7921 aaaatttaaa aggcgcaggt attacgacaa ttatgattac tggcgatcac gctgatacag
     7981 cttacgcgat tgcccatgaa cttggtatta ctgatagccc aagtaatgtt ttaactggcg
     8041 cggagcttga cgctttaaat gaagatgcct taaaagatgc tttgcttact aagcgtgtct
     8101 ttgcccgtgt atctcccgaa aacaaagtac aaattgttaa tgccttacag gctaatggtg
     8161 aaattgtagc gatgacgggg gacggtgtta atgacgctcc tagtttaaaa actgctgata
     8221 tcgggattgc gatgggtatt accggaactg atgtggctaa aggtgccgct gatatggtat
     8281 taactgatga taactttgcg agtattgaaa aagcggtgca agaaggacgc ggtatatacg
     8341 ctaatattcg taaaacagtg tggtttttat tatcaagtaa ctttggtgaa gttattacga
     8401 tgcttgttgc cgtcttagta ggttatcctt tcccgctttc ggcattacat attttatggg
     8461 ttaatttaat tactgatggt gtaccggcgc ttgcacttgg cgctgactcg aaagacccag
     8521 acattatgaa agaaaaaccg cgtgataaga aagaatcatt attcgctaat ggtggttact
     8581 ttattacgat attcttcgga agtgttattg ctttaattac tactttggcg ttcttattta
     8641 tcccggtagt gcaacacggg atgcgtagtg ttcaagatat taatactttc tttgcggaaa
     8701 atcctgaact taaagttaga gcaatgactt atgcttttac tgcattaggt gttagccaac
     8761 tcttccatat gtttggcatg tcaaatttaa ataaaacatt ctttagaaca tttacgaaga
     8821 aaaagtggtt cttatgggtt gctttcttta ttggcttcct tttacaaatt gcggtaactg
     8881 aaattcatgc tttaactgtg gcgtttggta cagttgaatt aacatttatt gagtgggtat
     8941 ttttagcatt acttagtaca acaccacttg taattcatga attaagagtt ttcttccgct
     9001 ttatccgtga gaaaatcaaa aatcgaaaaa gtcattaata acactattgc tttatgacat
     9061 tagtaaagtt ataataaatt tccttgtgag gataagtata tgaaaaaacg aaatattttt
     9121 actaaatttt ctttaactgc aattttacta tctgcgctct ttgcttttag tagtgtacgc
     9181 gctagtagta atatagttag aacagaagcg tggagcggga cacaaactag tactgaaggt
     9241 acatattact ccaccgttgg tagtgaaacg ggcacagctt tacttaataa attaaataca
     9301 attacgggta atccgactcc gcaaactagt tatgattggt atcgctacga agcagcggat
     9361 gaggcggaaa atgagagcaa taaagtgctt ttaatttatt cgcgacaaaa agtacttaaa
     9421 acggcccatg tttcaggaag tacaggttgg aatagagaac atacttatcc tgactccaaa
     9481 atatcgggtg ctgccaaatc ggacaaccat catattttcg ctgatgacaa tcgcacaaat
     9541 agtactcgtg gcaacatgcc atttggtgtt gttgctcaaa ctagcacaaa tcgggttaaa
     9601 gacggttatg gaaatttgac agattgctat aaagatggta cttattttta tccaaacgaa
     9661 cccgctcgtg gtgaagtagc gcgcgctacg atgtatgtta atatgcgtta tggttatagt
     9721 gtgactggta acttccaaag tattgcactt atgctagagt ggcatttaca aaatcctgta
     9781 tcaaatcgcg aaatatatcg taataatacc gtccatacaa tgcaaaaaaa tcgtaatcct
     9841 tttattgatc atccagaata tgcatgtcgg atttggggta acactaatag tgaaacacaa
     9901 gctatttgcg gggctgcaca acaagtcgat gttactagtg taagtgtgtc accgtcaagt
     9961 ggtagcatta acttattaga tgttaataag acattacaat taagtgcgaa tgttttaccc
    10021 gctaacgcta ctaataaaag tgttaactgg agcacttcag actcaagtat tgcgactgtt
    10081 gacgctaacg gtttagtcac gggtaaagca gtgggaaacg tcacaattac tgctacttcg
    10141 gtatcaaatc catcagttaa aggtacagca tctattgctg ttagtaatac atcaattcct
    10201 gttactggtg tcactattag tgataaatcg ttcacattag ttaaagacgc tactaaaaca
    10261 ttaactgcaa cagtaagtcc agcgaatgca tcaaataaga atgtaacatg gtcatcatca
    10321 aatacaagtg ttgcgactgt ttctagtagc ggggtcgtta ctggagtaag cgcaggaaac
    10381 gccacaataa ctgtcacaac agttgatggt ggtaaaactg ataccgcaaa cgtaacagtc
    10441 gccaatgcac cgccaaccca aagtgaaaca acaacttata cattttcaac caaaggttgg
    10501 aaagctacac ctgctaattg gcagagtatg tatcctgcta atggttttga aactgatcaa
    10561 aactatttaa gaggtatgca ggttttaggt aataatacag cctatggtaa tagtccgtat
    10621 ttcgataatg tgaaaaaggt tatttttgga acagcagctt caagtagtgg ggcaggaact
    10681 tatagtgctt atctagtaac ttctagtacc tcaaaattaa aagatgggac acagattggt
    10741 accgatgtaa ccgttagtaa accaggtgcg actattattg agcgtacttt tgaattaaca
    10801 acaccagcgt cgggtcacgt tcaagctatt attgacgcga cttctaattc tttgtactta
    10861 aaatatgtaa cgattgaagt agcgacaccc attaattatc aaggggaggc tgatttatgg
    10921 gcaaatggct tcttagaaag tacaagtgct ggttgtagta gccaatcaca aagtacatta
    10981 agtagtattt ggccaaattt aagtaatgag tataacgcat tagcagcaga agttaaagca
    11041 attattgaag gctcaacacc aaactctgct ggtagtgaag ttgaacaagc tttagcgcgt
    11101 tatgtgttta ttgttaataa atatggattt actgccttta ttaatgggat ccccgctagt
    11161 ggtgtgcatg acacaatgca taatgataaa caattagcgg aaccgctttt aattttgttt
    11221 agtgtagcaa cgcttagctt aatcccgttt gtgtataaac gcaaaaaacg aacacttaat
    11281 taatataaaa atttaattac aagaaaagcc gaggtctgcc ccggcttttt aacaatagaa
    11341 gtatatgtta gcgccaatag tggtcgaaga atttagtagc gtagtttgcg tgaagttcat
    11401 agcgcgatcc tggagcaaaa taagtgttaa gcacaccgtt atattcagtt tccataacga
    11461 ttgaaggatc aacaactttt ccgacttcac tttcatattc tcctaaatag cgggtagctc
    11521 tcacaccatt gtagtaggag tcttttattt tataaacacc atctactttt ttaacttggt
    11581 cattttggaa gacgagcatg tcgtcaatca tcggactaat gtcattattc gctaaataag
    11641 tagcgccatt accatctggt cgaataactt gtaaagtagg aacatagcct gtcgcgtaac
    11701 ctgaaacagt attattttta tcacttaagc cataaatgtc tttaacatta agccaattac
    11761 cagcgtcacg aattggttta ctttcgataa tgtaataagg catctttggc gcttttttaa
    11821 gtgtttcaat gtaacccggc ataaactgtt tatcaagggc cacgcagtct ggacaaatac
    11881 tatagctcca attaagtaaa aatggttccg tgccttttag tagtgtatta acttcttcaa
    11941 gactaatata agtcatcagc gggtctttaa ttcttgcgtc catccattca ttaaatttat
    12001 cgcgttcaat aaagatttca ctttttgatg tgtaatcaat actatattta caaacgccac
    12061 ctgcaaaaat accaataacc ggagtgttcg ttaaatcttt gcgaatcggt agtcctttaa
    12121 agtcgttacc taatgcatct gtttcaataa cataaattgg gattttcttc gtatttatgt
    12181 agtttcttaa tacatggtca cggaacacac tccagcatga acatgtataa tcagcgtaac
    12241 ttactaacac aaaattttct ttatcggtga tttttaattc aagctgttca agcgctgttt
    12301 ggttttcatc atcaacaatg tttattactt ccgttaagtt tgaatattta tacatgattt
    12361 ggactttttc catttttggt ggtttattgc aacctgttaa tagaaaagta cctaataata
    12421 agaatgctaa accctgtttt ttcataagtt tttacctcga tttcactcta tatgtgagtt
    12481 tattttatca agttttaata gtcgttgcaa catgcagaaa aaagggtgaa aaatttacaa
    12541 aaaaagaggg tgaaaaatag acccataaat tagacgaaaa aagggggtaa aaattcgaca
    12601 attttttatt tgaaatcttt atcatataat gcaataaaat ctgctttttc ggggtgccaa
    12661 aaaaagtgta aaataattat gcttgtattt tattaaaatt tgccttatat tttattgtgt
    12721 tgaaaggggg gtacctatgg acaaattaac aaagcataac gtgaagaaag aagacgttat
    12781 cattgaatta aaagatatta gtaaagagta tgatggaaag acagtagtgg atgattttaa
    12841 cttgtatgtg aagcgtggtg aattcattac atttttagga ccatctggct gtggtaaaac
    12901 aacgacttta cgcatgattg ccggttttga attaccgaca acagggacga ttacacttaa
    12961 cggagaagat attaccaaac tcccaccata taaacgtcca gtaaatacgg tatttcaacg
    13021 ctacgcatta ttcccacatt tagatgtgta tgacaatatt gcttttggct taaaacttaa
    13081 aaaagtcaaa gacacaaaaa acgggaaaga agtaatgcgt aaacttactt ttaaagaaat
    13141 tgatgaaaaa gttgtgcgcg ctttaaaaat ggttgatctt gaacaatttg aagcacgtga
    13201 tatcgcgacg ctctcaggtg gtcaacaaca acgggtcgca attgcgcgcg cgattgttaa
    13261 tgaaccagaa attttattac ttgatgaacc gcttggcgca cttgacctga agatgcgtaa
    13321 agaaatgcaa attgaattaa aatatttaca taaacgctta ggtattactt ttatttatgt
    13381 tacgcacgac caagaagaag ctttaacaat gagtgataca atcgtcgtga tgaatgacgg
    13441 acttatccaa caaattggaa cgccgttaga tatatataac gaaccaaaga acccgtttgt
    13501 tgctgacttt atcggtgaat caaatattta taatgcgaca atggcgggca agcttaaagt
    13561 gaagttttta ggcgcgacat ttgactgtgt tgatgactta ccgacaaatg aaaaagtcga
    13621 tgttgtgatt cgtcctgaag atattaaaat tgttgccgct ggtaagggta atgttgacgg
    13681 gaaaattatt agccgtgtct ttaaaggtgt gcactatgaa tatgatgtgc aagtaggccg
    13741 taaaagtgaa gttattattc aaagcactat tgaatatccc gaaaatgaaa aggtcggctt
    13801 agctgttgcc cctgatgata ttcatattat gaaaaaagaa gtgacaacaa atgtgtacgc
    13861 tgatgcatgg attgataata agaatcgctt aatgatcggt gataatgctt ttgaaatcga
    13921 tattactcag ctcttaaaag ggagcactgt tgatgaagaa ggttatttag tgggacctga
    13981 tgctaaaaaa tatgatttag atgatgttga tgtcgttgcc actattgact ttaaagatat
    14041 tgaacttgat gacgtcattg aagaagatgc gccaatggga caaataatca gcattatttg
    14101 gcttggtgac cactatcaag ttatcgttcg tactaatgac gaagaagact ttatcgttga
    14161 ttccgaatac ttatggaacg aaggtgactt agtaagtatt atcattaaac ctgaagatat
    14221 taagctaaaa ctaaaaggag aattagtgcc gtatgaaaat tagattcaaa cggtcctaca
    14281 ttgggattcc ctatgtgctc ttcatggtgc tcttcgtctt attaccgctt ttctttgtag
    14341 cattttacgc cttcactaac agtgcggggc gctttagttt tgagccaatg gtgaacttct
    14401 ttacaagtag tgcgaaatgg cgtgttttat tcgtttcctt tatttttggc atattaaata
    14461 cagcactatg cttagttatc ggctatccgt tagcgatgat tttagcgaat cgaaaatata
    14521 acactaacgc agtattagtg atgctctttg ttatgccaat gtggattaac ttcattattc
    14581 gctcatgggc gatgcgtgat gtacttgtgt tccttgggat tcgtggcggt gagtatccag
    14641 aacttgccac ccttttaggg ttaacttata actatttacc ttttaccatc ttgccgcttt
    14701 attcaacgat gctaaaaatg gataaaaatc aaattgaagc aagttatgac ttaggcgctg
    14761 gcccagtaac aactttcttt aaagtaattg taccaatgac gatgcccggg attattagtg
    14821 cagcgacaat ggtctttatg ccaacgatga caagttacgt tatcgcggat attttaggag
    14881 aaggtaaagt gacattattt gggaagtata ttgaaattta tttcacccaa aacatttgga
    14941 atgaaggctc attccttgcg cttgtaatgc ttgcgcttat cggtattagt gtccttgttc
    15001 ctaaactctt aacaagaaaa gaagcaaaag tgaagggggt cggtatttat gattaagaag
    15061 attctcgcaa aagtatatgt atacttaatt ttattccttt tgtacttacc aattttattt
    15121 ctcattattt atagctttac tcccgcggaa acaacaggag tatgggaagg ctttagtttt
    15181 gagctttatg gacgcgtctt tttaaatgaa aagattatgc gcgccttact taatacactg
    15241 attattgcgc ttagtagtgc aacggttgcg acaatcttag gcactcttgg ggcaattggt
    15301 gtcttttata tgaaaaagcg tccgcaaaga ataatggaag gaattaatga aatacctgtt
    15361 gttaacgcgg aaatcgttgt agcgattagt ttagctattt tattccgggt gttcgctaat
    15421 ttcttagaag cacgtggtgt agcgacaaat agtccaaact ttgtcacact tttaataggg
    15481 catgttgtgc ttagtatttc ttttgtgtat ctcaatgtta aacctaaact ccaacaaatg
    15541 gatccaaatg attatgaagc agcgcttgac ttaggtgcaa aaccgatgta cgcgattcgt
    15601 aaagtcgtgt tacccgcaat tgtacctggt attatcagtg gctttatgct tgcttttact
    15661 ttaagtttag atgattttat cattacaaac ttcttacgta atcagtcttt tgacaccctt
    15721 tcaacttatg tacaaggtgt gattgtgcgg gcagcaattc ccgccgagat gcgcgcccta
    15781 acgacttata ttgtgttagt cacactagcg gttgtgcttg tgtctgggat taaacgcgag
    15841 tcacgctctt cgcgcaaagc gatgctaaga gcacaaaaac gggaggtttt aactattaat
    15901 aaatcaagtt aaatgataag agcatagtaa aaaata
//
LOCUS       DAOSLP010000021        15710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_3912_length_15710_cov_8.556691, whole genome shotgun sequence.
ACCESSION   DAOSLP010000021 DAOSLP010000000
VERSION     DAOSLP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 15710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15710
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3912_length_15710_cov_8.556691"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1257
                     /locus_tag="PK460_02945"
     CDS             139..1257
                     /locus_tag="PK460_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE54083.1"
                     /translation="MKKSRLLAGFVLLAGIMLSVTGCREKTYKLALVTDVGTINDKSF
                     NQGAWEGLEKYAKDKKMTYQYYQPKQQTTNDYVESAKVAIENGAEVVVTPGYLFENAI
                     WQLQTEHPDVKFILLDGSPHNVIDWDRMATLDGEDPNYDIKDNVYSIFYAEEEAGFYA
                     GYASVKEGMRDLGFMGGMAVPAVVRFGMGYIEGAKYAANELELDDGAVTMKYTYLNSF
                     IPDAAHQTKAAAWYGTGTEVIFAAAGGAGGSVMKAAEEKQNKWVIGVDVDQRAESRTV
                     LTSAIKSLGSSVYQTLDKIYKDGVKGGTSETLDSTVGGVGLPAKSDADFDRFQNFNKA
                     QYDAVYELVKNGTITVYDDVEDPDYVALGGAKVVVTVE"
     gene            1375..2910
                     /locus_tag="PK460_02950"
     CDS             1375..2910
                     /locus_tag="PK460_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE54084.1"
                     /translation="MSYVIEMLNITKSFPGIKANDNVTLQLKHGEIHALLGENGAGKS
                     TLMSILFGLYQPDEGTIKVHGEVVNIKNPNDANDLKIGMVHQHFKLVEDYTVLENIIL
                     GREETKNGFLTTKNARKKILEIADHYGLHIDLDAKIEDITVGMQQRVEILKMLYRDAE
                     ILIFDEPTAVLTPQEIVELMDNMRDLINEGKSIILITHKLNEIKMVADRCTVLRKGQY
                     IGTVDVKNTTVEEMAEMMVGRKVSFHIEREKSEPGEVILEVKDVNHFNDYLNKNDLTD
                     VSFNVRAGEIVVVAGIEGNGQSQLASVITGLRNVKSGNIYLNGDEITRKTIRQKTLTG
                     ISHIPEDRHKHGLVLDFNLAYNLILQTYFTPEIQKYGFLKFDSINKKADELIERFDIR
                     SGEGRKTPMRSMSGGNQQKTIIARELSRPMDLVVAVQPTRGLDVGAIEYIHKQLIKLR
                     DERKAILVFSLELDEVFAIADRILVMFEGRIVANLITAETNENELGLYMSGSKEATYD
                     GSN"
     gene            2894..4126
                     /locus_tag="PK460_02955"
     CDS             2894..4126
                     /locus_tag="PK460_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE54085.1"
                     /translation="MTEVTKKRTCSLKFKTWWRKVVESTGIEKFLATIIAIIAGLLFG
                     LIVMLVINITDPFVAFEGLGAILTAGFSKGVKSIGEVLFRAAPLILVGLSVAFAFKTG
                     LFNIGASGQLMMGSFLAVFVGIKTESWILALISGVLGGALWALIPALLKAYRNVHEVV
                     ATIMMNYIAMYSSKMLIEWLIFMPIQQQARPVPTGGQLPTLGLDKLFNFPRLNSGIVI
                     AIITTIIIFVLLEKTTFGYQLKAVGFNREAARYAGINDKRNIVLSLVIAGALAGLAGA
                     VIYLVPAQGERLATKHVISNYGFQGIAVALLGMSHPIGTMGAGLFFGYIEAANLPLQR
                     VGFKREIIDIISASIIYFSAFTLFFQIYARRFINKMRDFFAKIKKKPQLATAEGVEIE
                     TLPPNEEGKEGGQEDDRK"
     gene            4242..5225
                     /locus_tag="PK460_02960"
     CDS             4242..5225
                     /locus_tag="PK460_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE54086.1"
                     /translation="MYDLLTQTIYSAIPLAIVAMGGMYSERSGVVNIALEGTMMFGAF
                     VGILCLSHIQHAGIDGQHIIFLALLIGAISGVIFSALHAFASITMKSDQTISGTALNM
                     LAAGLAIFMGRALSLGRTEEITFSSTHHRISAIPLLRHIPFFGKILFEDIYLISLFGI
                     LLFIVMGVIAYKTKFGLRLRACGENPHAADAAGINVIRTRYIAVLISGMLGGIGGVLL
                     IIPTTVSFIANVYGFGFLALAVLISGQWKPSRIMIFAIFFGLLRSIAFGFAFIKADLS
                     HIAFLKTFLDKLPTDILNMLPYVVTLIVLAFTSLKSRGPKAAGEPYDQGKR"
     gene            5305..5381
                     /locus_tag="PK460_02965"
     tRNA            5305..5381
                     /locus_tag="PK460_02965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5339..5341,aa:Arg,seq:acg)
     gene            5572..6507
                     /locus_tag="PK460_02970"
     CDS             5572..6507
                     /locus_tag="PK460_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008753856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE54087.1"
                     /translation="MSKINEIQVLLSKKAELNARLSLLPYDGTPEIKTISGKDYLYVR
                     KRVLNKVTSTYVDVYSDKLYNNLLRNNEEAKKLRREIRKIDKALASFGYKEGILLPNV
                     ILARDFARKNRNAIIYDQVILEGIGTTFPETEAILEQGIIKNARAEDVQKILNLKRAW
                     EFILDDDVLLTKTDFHLISYIAKIVNEGLFIFGGEVRGVSVKIGGSTYYPPIPLESKV
                     KEDINALLSSSLNDVDTAIELCLYIMKTQIFNDGNKRTAVIATNHYLVSKGIGFLAIA
                     YDKVPAFREMLVNYYENKNINEIKNFLKSCIHLVD"
     gene            6676..7149
                     /locus_tag="PK460_02975"
     CDS             6676..7149
                     /locus_tag="PK460_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE54088.1"
                     /translation="MTKEMFSEKYLTYKVMVFQISYAYLRNIEDAEEATLDSFMKFYK
                     STKKFQSVNHEKNYLIRVTINTAKDYLKKIKALPLNEEVHGVYDDNGELDKHYVWAVV
                     DELEGKYKDVIYLRYTADLPYKDIASILKISEALARKYHEIAIKTLRELIGGNYA"
     gene            7142..8491
                     /locus_tag="PK460_02980"
     CDS             7142..8491
                     /locus_tag="PK460_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54089.1"
                     /translation="MPELEKKLHETKTLIPEVPQRLDIKMEERIDGATKVKRPLNLRF
                     RFVLATAIVACVALSATTIALFSHTQKEQQQLFHKLPQSKVLMNLDDDLVNRTAIKAV
                     EKLSPFLTDEKAEDTVLSPATYSLAMAGLSAVSDNFNNDAFGLINAEEDVKALLEAWN
                     FKFKNYTGETFFRSAVLHQQVGPTYAFDKSKQEAVSDHYISTMVSSHESYRKDADKFF
                     DKKIGLKLKVPDLNLKSDGVVTYGVLKMKDYVANDGLFHRHMPFTFKSGLGTADSYVF
                     AVREDPKKVAYYRGGNYEVFKVNIRNTDLMIVLPNEEVNINHVNITEAYTNFVNEAEL
                     VPAFGYVPFFHNKVEGLNLTDTLENVITHNEIYMSKLLKDDVYNDLVIDAVVQSNDFE
                     FNKYGVAGESMTAVSGCTSTGPLYDEPIDLSVNRPFYAFSLKDKYPIFINKVSKPGA"
     gene            8715..9854
                     /locus_tag="PK460_02985"
     CDS             8715..9854
                     /locus_tag="PK460_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE54090.1"
                     /translation="MIVLNLKLDNILAFKDFEVNFSYPRKLKKTLIEGEHLRTIPTFR
                     YKKLVVLIGSNATGKTSLIKLIWRISLFLHRKNPSFLDSLISENNEHGNIEIDFVNEY
                     DDVAILYRAKIRIIKNNVNNQNIVEVAMYSKKLTEGDSYENSINELDNHEYVFESWQV
                     ALQEFYDNFGWQVVLPSNEELFDKFYIHADINESEKDDYLDILNNVMKTLDPAIKNVF
                     HSKDAADAIVIEYSDERKVLIQNGNDISSIPLLSSGTKYGVAISNILFSIKNHRNGLY
                     LVDEQFSYVTADIETAILAKMTALLNSGEQLFFTTHNSNILELGFPFHSFYFMKKTII
                     DGAPIINVSCASELENRNNVSPKNMFDNDAFGTAPNVDGIYSIGK"
     gene            10039..10209
                     /locus_tag="PK460_02990"
     CDS             10039..10209
                     /locus_tag="PK460_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54091.1"
                     /translation="MSNDEWWTYSEEYDCAVPTAKCPAKGIREFNRNRNLITGFIMIG
                     DGSVPPEYDPYI"
     gene            10413..11612
                     /locus_tag="PK460_02995"
     CDS             10413..11612
                     /locus_tag="PK460_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE54092.1"
                     /translation="MKITFMLFYYDPENPTINYLYRPLWDKLIKAGHEINIITPNPTR
                     GIPLSEQEKYKKTSVEKKGAFTIHRVKCYTYHPSRFSKFKLLRRYISVSRRLAKKLRK
                     VESDVVFVSSNPPLLYSYLVTRYCARKKIPVIYNVQDIYPDNIFKENSFAYKVFNPLQ
                     VKSLARASEVVTISETMRKTLLKKGDFNNKISVIYNFDISEVRKTPLVKKYTFFDPLQ
                     FNVVYAGNIGYVQDIDVILSAAKLLVHEPRIHIHIVGEGSQAARIAKRIHDENIVNAT
                     YYPPHSVEDSAQLYKNADVNVISILPGIIKTALPLKTASCLAAQKPIIFIGLETFYHN
                     EEWVNEVSELYPINYRNYEKVAETIEKLANAKTKPKLKYDQIELFDKEKNVNEYFNLI
                     TKYLKEE"
     gene            11861..12253
                     /locus_tag="PK460_03000"
     CDS             11861..12253
                     /locus_tag="PK460_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54093.1"
                     /translation="MLIYIQLLLDYYSKAFEGLFAFHEWPKALIDKYLIDNNVELTIP
                     NVPNIEFCLAGVGYGQVSVVIPGPDRCSEIKDVFEEQNFTVEHVVENNYYKVINLEGN
                     FEMMFNYYTEPKVTPNYPGFVNMVLYQP"
     gene            12500..12576
                     /locus_tag="PK460_03005"
     tRNA            12500..12576
                     /locus_tag="PK460_03005"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12534..12536,aa:Pro,seq:tgg)
     gene            12582..12657
                     /locus_tag="PK460_03010"
     tRNA            12582..12657
                     /locus_tag="PK460_03010"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12615..12617,aa:Ala,seq:tgc)
     gene            12667..12744
                     /locus_tag="PK460_03015"
     tRNA            12667..12744
                     /locus_tag="PK460_03015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12702..12704,aa:Met,seq:cat)
     gene            12761..12836
                     /locus_tag="PK460_03020"
     tRNA            12761..12836
                     /locus_tag="PK460_03020"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12794..12796,aa:Ile,seq:cat)
     gene            12840..12930
                     /locus_tag="PK460_03025"
     tRNA            12840..12930
                     /locus_tag="PK460_03025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12874..12876,aa:Ser,seq:tga)
     gene            13040..14197
                     /locus_tag="PK460_03030"
     CDS             13040..14197
                     /locus_tag="PK460_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54094.1"
                     /translation="MKAKIITMLTFCLLMLVSCNDKTSSSNIPSEEVTSTQVTSTPTS
                     TSFPSSEPLEESSDEPLTSEASSNTPSSEQSTINEELQFISNSSRYGNTNGNTMNRSL
                     AVYDFERKMHYYANRGSIYAYNPVTATSTELLADMSSITYLTLYENTIYFIGSTENYL
                     YTYDLLSMTTDLVLERGVSYASRQTNYLYLKTTIEGFGDDFYFVCYNLSNMTMIKYSS
                     MLDNINHVWNKVIYTDSEALKIKLVSYNLSGRTTIANFTERGFKALNYLHLLSEDYVD
                     EHNRLFFVSLQTQEGPAYYFYEIKTDTLTFICKGGAHSVNNDDKYLYLVIDRDIVIYN
                     YVEKTYERTIYFDMSVRNLNVINHYLYYQSGDHNDLYMYHPEMVVPFCLTA"
     gene            14748..15044
                     /locus_tag="PK460_03035"
     CDS             14748..15044
                     /locus_tag="PK460_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54095.1"
                     /translation="MLFSRRIFHQYEKPFYSKDGVEITPDWTLPQYKDLGDVIIEYWG
                     ITNDEKYEESKKYKLDIYKKEGVTLISIEQSEIKNLAEILERKLKTAKKGKINS"
     gene            15080..15601
                     /locus_tag="PK460_03040"
     CDS             15080..15601
                     /locus_tag="PK460_03040"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOE54096.1"
                     /translation="MEIIKSLKDSEYPKAALYNARIAARGVCLNAKGEIALTHILATD
                     KFGHRDYYELPGGGKRINETVLESAIREMDEELGVKVELLAKIGIIHDFYNLIKQENY
                     SYYYLFKVTKVGQRHFEERESRLIDKIVWVPIQKAILLYKEQLKIKGVGLLVARRELP
                     ILELVAKSFDKYV"
     gene            15629..15704
                     /locus_tag="PK460_03045"
     tRNA            15629..15704
                     /locus_tag="PK460_03045"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15662..15664,aa:His,seq:gtg)
BASE COUNT         5299 a         2410 c         3036 g         4965 t
ORIGIN      
        1 aaaaatgatt ctttcagttt tttatcttgt caatttaacc ttttcttcta ttattctaaa
       61 acatttttat tgttaaacac tttttttatg atatgctaaa tatgtagacc acatataaca
      121 taataaggag gaactttaat gaaaaaatct cgtctacttg ccggttttgt attacttgct
      181 ggcattatgc tctctgtcac tggttgtcga gaaaaaacct acaaactagc gctcgttact
      241 gacgttggta caatcaatga caaatcattc aaccaaggtg cgtgggaagg attggaaaaa
      301 tacgcgaaag ataagaaaat gacttatcag tattaccaac caaaacaaca aaccacgaat
      361 gactacgttg aatctgctaa ggtcgccatt gaaaacggcg cggaagttgt tgtaactcca
      421 ggctacttat tcgaaaatgc gatttggcaa ttacaaactg aacacccaga tgtgaaattc
      481 atcttacttg atggttctcc acataacgtc attgactggg atagaatggc aacacttgat
      541 ggtgaggacc caaattatga cattaaagat aacgtgtatt caattttcta tgccgaagaa
      601 gaagcaggct tctatgctgg ctatgcttca gttaaagaag gcatgagaga tttaggattc
      661 atgggcggta tggccgttcc agcagttgtc cgcttcggta tgggttatat tgaaggggca
      721 aaatacgctg ctaatgaatt agaattagac gatggtgctg tcactatgaa atacacatac
      781 cttaactcct ttattcctga cgctgctcac caaactaaag ctgctgcgtg gtacgggact
      841 gggaccgaag ttatcttcgc cgctgctggt ggagctggtg gtagtgtgat gaaagctgcc
      901 gaagaaaaac aaaataaatg ggttattggt gttgacgttg accaaagagc agaatcaaga
      961 acagtcttaa catccgctat taagagttta ggctcaagtg tgtaccaaac cttagacaaa
     1021 atctataaag atggcgttaa aggtggtaca agtgaaactt tagactcaac cgttggcggt
     1081 gttggtttac cagcaaaatc ggatgctgat tttgatcgct tccaaaactt taacaaagca
     1141 caatatgatg ccgtttatga actcgttaaa aatggtacca ttactgttta tgacgatgtt
     1201 gaagatccag attatgttgc cttaggtggc gcaaaagttg ttgttactgt tgaataataa
     1261 tttttaacat aagctaacta aaggccgcgc aattatacgc gcggcctttt actttattac
     1321 caaattaaaa acttcatgtt ataattgatt taaagttgta aaggagattt tattatgtcc
     1381 tacgttattg aaatgttgaa cataacaaag agcttccctg gcattaaagc taatgacaat
     1441 gttacgctgc aattaaagca cggagaaatt catgctttat taggagaaaa cggtgcaggg
     1501 aaaagtacat tgatgtcgat tctttttggg ctatatcaac ctgatgaagg aacaattaaa
     1561 gtgcatggtg aagttgtcaa cattaaaaat ccaaatgacg ctaatgattt aaagattggg
     1621 atggtccatc aacattttaa gttagtggaa gattatactg tgttagaaaa tattattctt
     1681 ggtcgagaag agacgaaaaa cggttttcta acaaccaaaa atgcacgtaa aaagatctta
     1741 gaaattgctg accactatgg tttacacatt gatttagacg caaagattga agatattact
     1801 gttggtatgc aacaacgtgt ggagatttta aagatgcttt atcgtgatgc ggaaatttta
     1861 atttttgacg aaccaacagc agtgcttacc ccgcaagaaa ttgtggagtt aatggataat
     1921 atgcgtgact taattaatga aggtaagtca attattctta ttacgcataa acttaatgaa
     1981 attaaaatgg ttgctgatcg ctgtacagta ctgcggaaag gacaatacat tgggacagtt
     2041 gatgtaaaaa acacaacagt tgaagaaatg gcagagatga tggttggacg caaagttagt
     2101 ttccacattg aacgcgagaa aagtgaacct ggtgaagtta tccttgaagt aaaagatgtt
     2161 aatcacttta atgattattt aaataaaaat gacttaacag atgtctcctt taatgtgcgc
     2221 gctggagaaa ttgtcgtcgt agcggggatt gaaggtaatg gtcaaagtca actcgcaagt
     2281 gtgattactg ggttgcggaa tgttaagtca gggaacattt acttaaatgg tgatgagatt
     2341 acacgtaaaa caattcgcca aaaaacacta acgggtatta gccatatccc agaagatcgc
     2401 cataaacacg gacttgtttt agattttaat ttagcgtata accttatttt acaaacatat
     2461 ttcactcctg aaatacaaaa atacggtttc ttaaaatttg atagtattaa taaaaaggcc
     2521 gatgaactaa ttgaacgctt cgatattcgt agtggtgaag gtagaaagac gccaatgcgt
     2581 agtatgagtg gcggtaatca gcaaaaaaca attatcgctc gtgaattatc taggcctatg
     2641 gatttagtcg tcgctgtcca accaacacgc ggacttgatg tgggggcgat tgaatacatt
     2701 cataaacaac taatcaaatt acgtgatgaa cgcaaagcaa ttcttgtttt ctcccttgaa
     2761 ctagatgaag tcttcgccat tgcggaccgc attttagtga tgtttgaagg acgaattgtc
     2821 gctaatttaa ttacagcaga aactaatgaa aatgaacttg ggctttatat gtcaggttcg
     2881 aaagaggcaa cttatgacgg aagtaactaa gaaaaggaca tgtagtctaa aatttaaaac
     2941 ctggtggcgc aaagttgttg aatccacagg gattgagaaa tttcttgcga cgattattgc
     3001 gattatcgct gggcttctat ttggccttat cgttatgctc gtcatcaata ttactgatcc
     3061 atttgtcgct tttgaaggac ttggggcgat tttaactgct ggtttctcta aaggcgtaaa
     3121 aagcattggt gaagtgctct tccgcgctgc accattaatt ttagtggggc tttcagtcgc
     3181 atttgccttt aagacaggct tatttaatat tggcgctagt ggacaattaa tgatggggtc
     3241 gtttttagct gtttttgtgg ggattaaaac cgaatcatgg attttagcgc ttattagcgg
     3301 tgtgctcggt ggcgcattat gggcattaat cccagcctta cttaaagctt atcgcaatgt
     3361 ccatgaagtt gtggcgacca ttatgatgaa ctatattgca atgtactctt caaagatgtt
     3421 aattgaatgg ttaattttta tgccaattca gcaacaagca cgtcctgtgc ccacaggcgg
     3481 acaattacca acattaggac ttgataaatt atttaacttt ccgcgcctta atagcgggat
     3541 agtaatagcg ataattacga caattatcat ttttgtgtta cttgaaaaaa caacctttgg
     3601 ttatcagctt aaagcagttg gctttaaccg tgaagccgcg cgttatgcag ggattaatga
     3661 taaacgtaat atcgtgctct cacttgtaat tgctggcgct cttgctggtt tagcgggggc
     3721 tgttatttac ttagtgccag cgcaaggcga aagacttgcc acaaaacacg ttatctcaaa
     3781 ttacggcttc caagggattg ccgttgcact ccttgggatg agccatccaa taggaacgat
     3841 gggagcgggg ctcttctttg gttatattga agcggcgaac ttaccactcc aaagagttgg
     3901 gtttaaacgc gaaattattg atattatttc ggcaagtatt atttacttta gtgcctttac
     3961 gttattcttc caaatttacg cacgacgctt tattaataaa atgcgtgatt tcttcgctaa
     4021 gataaagaaa aaaccacaat tagcaactgc cgaaggagta gaaattgaaa cgctaccacc
     4081 gaatgaagaa ggtaaagaag gaggacaaga agatgaccga aaataaatca aagttcaaag
     4141 tttttcttgc taaacttccg aaactttcga aaaaacaaat tattacaatc gcagttttac
     4201 ttgtccttta tgtgttactt gctatcttta cgagtgataa tatgtacgat cttttaacac
     4261 agacgattta ctcggcaatc cccttagcaa ttgtggcgat gggcggcatg tatagtgagc
     4321 gttcaggcgt cgttaatatt gcccttgaag ggacaatgat gtttggggca tttgtcggaa
     4381 tattatgctt aagtcatatc caacatgcgg gaattgatgg ccaacatatt attttccttg
     4441 cgctcttaat tggggctatt tctggcgtta tattctccgc cttacacgct tttgcctcaa
     4501 ttacaatgaa aagtgatcaa acaatttcgg ggacagcact taatatgctc gctgctgggt
     4561 tagcaatctt tatgggccgc gcactttcac taggccgaac agaagaaatt accttttcaa
     4621 gtacgcatca tcgtattagt gcgattccgc ttttacgcca tattccgttc tttggtaaaa
     4681 tcctgtttga agatatttac ttaatttcat tattcggtat tttgctcttt attgtgatgg
     4741 gtgttattgc ctataagacg aagtttggat tacgcttacg agcatgtggc gaaaatccgc
     4801 atgctgcaga tgcagccgga attaatgtca tcagaactcg ttatattgct gttttaatca
     4861 gcggaatgct cggcggtatt ggcggtgtct tacttattat cccgacgacc gtgagtttta
     4921 tcgcgaatgt gtatggattt gggttcttag cacttgccgt tcttatcagt ggtcaatgga
     4981 aaccaagtcg gattatgatt tttgcgattt tctttggact cttacggagt attgcttttg
     5041 gttttgcttt tattaaggca gacttaagtc atattgcgtt ccttaaaaca ttcttagata
     5101 agttaccgac ggatatttta aatatgttgc catatgttgt aacacttatt gtgcttgcct
     5161 ttacatcact taaatcacgc ggaccaaaag ccgcaggtga gccttatgac caagggaaac
     5221 gctgattatt tgctaaatat tacatattta actttataac acttgcaaaa atcacttaaa
     5281 taactataat gtttaagtga cgctgcggtc atagctcaac tggatagagt gccagactac
     5341 gaatctggag gttgggagtt cgattcttcc tgaccgcgcc attgcgaata aacatgctcc
     5401 ctaaaattgc actagagtct agtacaatga aaaggggagc tttttattta tgaaatctaa
     5461 ttatgtctaa tacatgcgat aaacgaataa atttgacaag gtaaaattta gatttgtttt
     5521 agggaacaaa aggtgtataa ttaagttaga aagttcccta agagaattgt tatgagtaaa
     5581 ataaatgaaa tacaagtatt attatcaaaa aaagctgaac taaacgcacg gctatcgtta
     5641 ttgccgtatg atggcactcc tgaaataaaa acaatcagcg gcaaagatta tctctatgtg
     5701 cgaaaaagag ttctaaataa agtgacttca acctatgttg atgtttatag tgacaagctt
     5761 tataacaatc tacttcgtaa taatgaagaa gcaaaaaaac ttaggcgtga aatacgcaaa
     5821 atcgataaag ctttagcgag ttttggttac aaagaaggaa tactattacc taatgtcatt
     5881 ttagcgcggg attttgcgcg aaaaaatcgt aatgcgatta tttatgatca agttatatta
     5941 gagggaattg gtacaacttt tcccgagaca gaagcaattc tcgaacaagg tattatcaag
     6001 aatgctagag cggaagatgt gcaaaaaatt cttaatttga aaagagcgtg ggaatttatt
     6061 ttagatgacg atgttttatt aacgaaaaca gattttcatc ttattagtta tatcgctaaa
     6121 attgttaatg aaggattatt tatttttggt ggcgaagtta gaggggtatc tgttaagatt
     6181 ggtggttcaa cttattatcc tcctatccca cttgaatcta aagtaaaaga agatatcaac
     6241 gctttattgt cttcatcgtt aaatgatgtc gataccgcta ttgaactttg tttatacatt
     6301 atgaaaacgc aaatctttaa tgatggcaat aaaagaactg cggttattgc cactaaccat
     6361 tatttagtga gtaaaggcat tgggttttta gcaatagctt acgataaagt tcccgctttt
     6421 agagaaatgt tagtaaatta ttatgaaaat aaaaatatta atgaaattaa aaactttctt
     6481 aaaagttgta tacatttagt tgactagtgc aaacttagca atataaaggg ttttttaaca
     6541 atatttacta ttttttcgca aaacagtgtt tttaatcgta ttataagtag taggtattaa
     6601 taataatgga aacaaataaa gtgttgatga attcacaaaa gtgtcgcgtt aatcgtaata
     6661 atagtgtagg tgcttatgac taaagagatg tttagtgaaa agtatctaac ctacaaagtc
     6721 atggtgtttc aaataagcta tgcatattta cggaatattg aagatgctga agaggcgacg
     6781 cttgactcat ttatgaaatt ttataaaagc acgaagaaat ttcagtcagt taaccatgaa
     6841 aagaattatt taattagagt aacgattaat actgcaaaag attacttaaa gaaaattaaa
     6901 gcgttaccct taaatgaaga agttcatggt gtgtatgacg ataatggtga acttgataag
     6961 cattatgtat gggcagtagt cgacgagtta gaaggtaaat ataaagatgt tatttatttg
     7021 cgttatactg cagacttacc gtataaagat atagcaagca ttcttaaaat aagtgaagca
     7081 ctagcgagga aatatcatga aattgccatt aaaacattgc gtgaattaat cggaggaaat
     7141 tatgcctgaa ttagaaaaga aattacatga aacaaaaaca cttattcctg aagtgccaca
     7201 aagattagat attaaaatgg aagaaaggat tgatggtgct acaaaagtaa agcgtccgct
     7261 taacttgcgt tttcgctttg tcctagcgac agccattgtc gcgtgtgtgg ctttaagcgc
     7321 gacgacaatt gccttattta gtcatactca aaaagaacaa caacaattat ttcacaaatt
     7381 accacagtct aaagtgctga tgaatttaga tgatgactta gtaaatagaa cagcaattaa
     7441 ggcggttgag aaattatccc cctttcttac tgatgaaaaa gcagaagata ctgtgctatc
     7501 accagcaact tactccttag cgatggctgg gctttccgct gttagtgata actttaataa
     7561 tgatgctttt ggtttaatta atgccgaaga agatgtaaaa gcgctcttag aagcatggaa
     7621 ttttaaattt aaaaattaca caggtgaaac ttttttccgc tcggcagtct tacatcaaca
     7681 agttggacca acttatgctt ttgataagag taaacaagaa gcagtaagtg atcattacat
     7741 ttcgacaatg gtctcttcgc acgaatctta ccggaaagac gctgataaat tttttgataa
     7801 gaagattggt ttaaagctta aagttcctga tttaaatctt aaaagtgatg gagtagtgac
     7861 ttacggtgtt ttaaaaatga aagattatgt cgctaacgat gggctttttc atcgacatat
     7921 gccttttaca tttaaaagcg ggctaggcac agcggattcc tatgtctttg ctgtaagaga
     7981 agatccaaag aaagttgctt attatcgcgg aggtaattat gaggtattta aagtaaatat
     8041 tcgaaatact gatttaatga tagttttacc aaacgaagag gttaatatta atcatgtaaa
     8101 tatcactgaa gcttacacta attttgttaa tgaggctgaa cttgttcccg cttttggcta
     8161 tgtacccttc ttccataaca aagttgaagg tctaaattta acagacacat tagaaaatgt
     8221 tattacgcat aacgaaatct atatgagtaa attacttaaa gatgatgttt ataatgattt
     8281 agttattgac gctgttgtgc aatcaaatga ctttgaattt aataaatatg gtgtggcagg
     8341 agaatcaatg accgcagttt ccggctgtac atcaactggt ccactttacg atgaaccaat
     8401 tgatctttct gttaatcgtc cgttttatgc cttctcttta aaagataaat acccaatctt
     8461 tattaataaa gtgtcaaagc ctggcgcata attattgttt gtcataaaac tatttactaa
     8521 aaataaggct acatcaaaat gtagtctttt tttaaaaatt gattttaatt tattggtcgc
     8581 tgcatattct atttaagtat ttaacatttt tttaaaaagt tttaaaaagt ttgaaaatac
     8641 ttgtttttca aattaaaatt tattacatta aaattgtaag ttcaaaaaac ttgaatatac
     8701 aaggagcaaa taatatgatt gtcttaaatt tgaagttaga taatattcta gcctttaagg
     8761 atttcgaagt taattttagt tatccgcgta aacttaagaa aacgctaatt gaaggagaac
     8821 atcttcgcac aattccaact tttagatata agaaattagt agtgttaatt ggttcaaacg
     8881 caacagggaa aacttctctc attaaattaa tttggagaat ttctttattt ctgcatcgta
     8941 aaaatcctag ttttttagat agtcttataa gtgaaaacaa tgaacacggc aacattgaaa
     9001 ttgactttgt aaatgaatat gatgatgttg ctattttata ccgcgctaaa attagaataa
     9061 ttaaaaataa tgtaaataat caaaacattg ttgaagttgc aatgtatagc aagaaactca
     9121 ctgaaggtga ttcatacgaa aatagtatta atgagttaga taatcatgaa tatgtctttg
     9181 aaagttggca agtagcttta caagagtttt atgataattt tggttggcaa gtagtccttc
     9241 catcaaatga agaattattt gataagtttt atatacatgc tgatatcaat gaaagtgaaa
     9301 aagatgatta tttagatata ttaaacaatg taatgaaaac attagatcca gcaattaaga
     9361 atgtttttca tagtaaagat gctgctgatg cgatagttat tgaatatagt gatgagcgaa
     9421 aagtattaat tcaaaacggt aatgacattt caagtattcc gctgctttca agcggtacta
     9481 aatatggggt ggcgatcagt aatattcttt tttcaattaa aaatcaccgt aacggactct
     9541 atttagtgga tgagcaattt tcatatgtga ctgctgacat tgaaacagca attttagcaa
     9601 aaatgactgc tttacttaat agtggtgaac aactattttt tactacacac aattcaaata
     9661 ttcttgaatt aggttttcct tttcattcgt tctattttat gaaaaagaca atcatagatg
     9721 gtgcaccaat aatcaatgtg tcttgtgcat cagaactaga aaatcgcaat aatgtatcac
     9781 caaagaatat gttcgataat gatgcttttg gaacagcgcc gaatgttgac ggtatctatt
     9841 ctattggtaa atgaaataca tgctgcaatc caaaattctg gacaataaat atgaatattg
     9901 agttctcatt tctatttttg aaaagaaatt tagtttcatg tgcataagaa tacacttgac
     9961 tctattattt gtaagagtgt aagattaggt aaaagcgagg aataatgaaa agtgactgat
    10021 ttaacacgat atatatttat gagtaatgat gaatggtgga catatagtga agaatatgac
    10081 tgcgccgtgc caaccgcgaa atgtccggca aaaggtattc gtgaatttaa tcgtaatcgt
    10141 aatttaatta ctgggttcat tatgattggt gatggtagtg ttccaccaga atatgatccc
    10201 tatatataag tattgaaaag tttgttaaaa tgcgtgagtc aataggttac actccgcaac
    10261 aaataaaacg cgaaactaga attatctatg accgaaaaac acgtcgcgaa tattataaaa
    10321 ataaagcata aattttgtgt tattagtgaa taatgcggca aatcatactt aaatttctgt
    10381 tttattatta gtgtcgtgtt atactttaaa gtatgaaaat aacatttatg cttttttatt
    10441 atgaccccga gaatccgacg attaattact tatatcgtcc gctttgggat aagctaatta
    10501 aagcggggca tgaaattaat attatcaccc ctaatccgac acgcgggatt ccgttaagtg
    10561 aacaagaaaa atataagaaa acgagtgttg agaaaaaagg ggcgtttacg attcatcgtg
    10621 taaagtgtta cacttatcac ccgagtcgct ttagtaaatt taaattactt agacgctata
    10681 tcagtgtgtc gcgccgttta gcgaaaaaat tgcgtaaagt agaaagtgat gttgtctttg
    10741 tttctagtaa cccgccactg ctttatagtt atttagtaac aaggtattgt gcgagaaaga
    10801 aaataccagt tatttataat gttcaagata tttatcctga taatatcttt aaagaaaata
    10861 gttttgcgta taaagttttt aatcctttac aagttaaaag tttagcgcgc gctagtgaag
    10921 tcgtcactat tagtgaaaca atgcgtaaaa cacttttaaa gaaaggcgac tttaataata
    10981 aaataagtgt catctataac tttgatatta gtgaagtgcg gaagactccg ctagtgaaga
    11041 agtatacatt ttttgatcca ttgcaattta atgtcgttta cgctggtaac attggctatg
    11101 tccaagatat tgatgtcatt ttaagtgcgg caaaactttt agtgcatgaa ccgcgcattc
    11161 atattcatat cgttggtgaa ggtagtcaag ctgcaagaat cgctaaacga attcatgacg
    11221 aaaatattgt taacgctact tattacccgc cacatagtgt ggaagatagt gcccagcttt
    11281 ataaaaatgc ggatgtaaat gtaatatcaa tattgccagg gataattaaa acggcattac
    11341 cacttaaaac ggcaagttgt ctagcggcac agaaaccgat tatttttatt ggacttgaaa
    11401 ctttttatca taatgaagaa tgggtaaatg aggtaagtga gctctatccc attaattatc
    11461 gtaattatga aaaggttgcg gaaacaattg aaaaactcgc taatgctaaa actaagccaa
    11521 aattaaagta tgatcaaatt gaactttttg ataaagaaaa gaatgttaat gagtacttta
    11581 acttaattac taagtactta aaagaagaat agtgaactga ttattaaagc ttttttaagc
    11641 attatgaagc tatcctttaa tacattttct attatttgtt gcacgaacag cataattaat
    11701 ataaaatgcg attgtatgta agaaattttt ggagagtgat taggaaaaag accaaatttt
    11761 tatttttagc cgcacttttt gcattaacgc taacgagact atacggataa gctataactt
    11821 tttagactcc acttataatg aaagactagc gacaggtggg atgctaattt atattcaatt
    11881 actgcttgat tactattcga aagcttttga aggcttattt gcgtttcacg aatggcctaa
    11941 ggcacttatt gataaatatt taattgataa taatgttgag ctaactatcc ctaatgttcc
    12001 aaatattgaa ttttgtctcg cgggcgtagg ttacggtcaa gtttcagttg ttattcctgg
    12061 tccagatcgt tgttcggaaa taaaagatgt tttcgaagaa caaaatttta ctgttgaaca
    12121 tgttgttgag aacaactatt ataaagtgat taatcttgaa ggtaattttg agatgatgtt
    12181 taattattat actgaaccaa aagtaacacc gaattatcca ggatttgtca acatggtttt
    12241 ataccaacca taaaaataaa agaagcgatg atataatcca ttttcttaat aagcataaac
    12301 gcaaatttta aagagagtaa tagtctttta aatatctttt tatcttcaag aaacatgcat
    12361 atttgataaa aaaagataat ttaaccgcta atcaattaat agataaatta accttttcga
    12421 ttgttagtgc aagaaagcaa gaactcaagt ttgttaatac ttgctaaatt tttatacctt
    12481 tggtaatata tacaagcatc gggatgtagc gtagcttggt agcgcgtctg ttttgggaac
    12541 agaaggtcgc aggttcaaat cctgtcatcc cgaccattta tggggccgta gctcatctgg
    12601 gagagcgctt gccttgcacg catgaggtag cgagttcgat cctcgtcggc tccaccatta
    12661 tattgtggct gcgtagctca gttggtttag agcggctgac tcatacccag caggtcgggg
    12721 gttcaaatcc ctccgcagct accaatacta aaattaagct ggacccttag ctcaatggtt
    12781 agagctcccg gctcataact ggttggttat aggttcgagt cctatagggt ccaccatacg
    12841 gaagcatacc caagcggttg aaggggtcgg tcttgaaaac cgatagggga tgcaagtccc
    12901 gcctgagttc gaatctcagt gcttccgcca gaaaattaat atatgctcat atattgagca
    12961 ttttttatta tcatataaaa tgttgtattt ctctaatatt aaaaatgcta caataaattt
    13021 aaggagaaac aaaaacttta tgaaagccaa aataattacg atgttaacct tttgtctttt
    13081 aatgcttgtt agttgtaatg acaaaacttc aagtagtaat ataccctccg aagaagttac
    13141 tagtacacaa gtgacttcaa caccaacatc cacctccttt ccaagtagtg aaccgcttga
    13201 agaatcaagt gatgaaccac taacaagcga agcatcaagt aacacacctt catctgagca
    13261 atcaacaatt aatgaagaac tgcaatttat atctaattca agtcgttacg ggaacacaaa
    13321 cgggaatact atgaaccgta gcctcgcggt atatgacttt gaacgaaaaa tgcattatta
    13381 tgcaaatcgt ggtagcattt atgcttataa tcccgtaaca gcgacaagca cagagttact
    13441 tgcagacatg tcaagtatta catatttaac gctttatgag aacactattt attttatcgg
    13501 tagtaccgaa aattatttat atacatatga cttattatca atgaccactg atcttgtgct
    13561 agaacgcggt gtaagttatg catcacgcca gactaattat ttatatttaa aaactacgat
    13621 tgaagggttt ggagatgatt tctatttcgt ttgttacaat ttaagtaata tgacgatgat
    13681 taaatatagt agtatgcttg ataatattaa tcatgtatgg aataaagtca tttatacgga
    13741 tagtgaagca ctgaagatta aattagtgtc ttataactta tccggtcgca ccacgattgc
    13801 taatttcact gaacgcggat ttaaagcact taattatttg catttattaa gtgaagatta
    13861 tgtcgatgaa cataatcgtt tattctttgt gtctttacaa acacaagaag gacccgcgta
    13921 ttacttttat gaaataaaga ctgatacact tacatttata tgtaaaggcg gagcacatag
    13981 cgttaataat gatgataaat atctttattt agtaattgat cgtgatatcg tcatttataa
    14041 ctatgttgaa aagacttatg aaagaacaat atattttgat atgagtgtcc gcaatttaaa
    14101 tgtgattaat cattaccttt attatcaaag tggggaccat aatgatcttt atatgtatca
    14161 cccagaaatg gtcgtgcctt tttgcttaac agcatagtag tgaggattga cactgattta
    14221 actccttaac tgggagtttt ttggatttaa taacttaatt tacagtttaa tattagaatc
    14281 taatctatac cagttcatac catgataatg ttatactaat gaaaatagga ggtagaacaa
    14341 tggaacaaaa atgtccaatt tgtggcaaga aaacatcatc ctatattggt aatgtgagaa
    14401 aagattgatt atgtcgagaa catgctaaac aactaaaaga aggaaaaata ataaaatgtg
    14461 aaaattgtgg ttcaattatt aaaagtgatg aaccttgtcc gaattgtgat ccagtaagtg
    14521 caactgtaat aattgattct caaaatactt gcataacatg tggtgaaaga actgatggat
    14581 atcatttttg taaggtttgt tataacaaat ataaagataa aaatataata gtcaatattg
    14641 caaaatgtaa ggaagcgaaa attttagaag aggattacta caacaccata tacaaaaccg
    14701 aggatggtca tctggtaaaa agtaaagatg aggttattat tgataatttg ctttttagta
    14761 gaagaatatt tcatcaatat gaaaagcctt tttattccaa agatggtgtt gagataacac
    14821 cggattggac attaccacaa tataaagatt taggtgatgt cattatagag tattggggaa
    14881 taacgaatga tgaaaaatat gaggaatcaa aaaaatataa attagatata tacaaaaaag
    14941 aaggagttac attaatttca atagagcaaa gtgaaattaa aaatcttgct gaaattttgg
    15001 aaagaaaact aaagactgca aaaaaaggaa aaattaatag ttgagttaat ttaaaatgca
    15061 tatttagtaa atttaaaaaa tggaaattat taaaagttta aaagatagtg agtatccaaa
    15121 agccgcttta tataacgccc gaatagcggc gcgcggtgta tgtttaaacg caaaagggga
    15181 aatagctttg acccatattt tagcaacgga taaatttggt caccgtgatt attatgaatt
    15241 accaggtggc ggtaagcgta ttaatgaaac agtgttagaa agtgctatcc gtgagatgga
    15301 tgaagagctt ggtgtaaaag ttgaactctt agcgaaaatt gggattattc atgactttta
    15361 taatttgata aaacaagaaa attattcgta ttattattta tttaaagtga caaaagttgg
    15421 tcagcgtcac tttgaagaaa gggaatcgcg ccttattgat aaaatcgtct gggttcccat
    15481 acaaaaagcc attcttttgt acaaagaaca actaaaaatc aaaggtgttg gtttgttagt
    15541 agcgcgtaga gaactgccaa ttcttgaact tgtggcgaaa tcctttgaca aatatgttta
    15601 agacaagtaa aatatctttt gctaactggc ggccgtggtg aagcggttaa cacacctggc
    15661 tgtgaaccag gcattcgggg gttcaagtcc cctcggtcgc cccagtttat
//
LOCUS       DAOSLP010000022        15585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_3937_length_15585_cov_9.109981, whole genome shotgun sequence.
ACCESSION   DAOSLP010000022 DAOSLP010000000
VERSION     DAOSLP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 15585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15585
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_15585_cov_9.109981"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1014
                     /locus_tag="PK460_03050"
     CDS             91..1014
                     /locus_tag="PK460_03050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE54097.1"
                     /translation="MKKKISLIIPCYNEEEMIDILYDEINKVLVKLPKYDFIFLFVDD
                     GSKDQTLSLIKAKAKKDHRVKYISFSRNFGKESSMLAGLEAAQKLDVDAALLMDADMQ
                     DPPSLMLEFFKYYEEGYKYIFTRNKTRKGQALLKRFFANAFYVIYAWLTGDKNSVHSA
                     RDFALLDRDVINAFLTYKDKHRYSKGISSQIGFKKKCIEYDFQERQAGTTKWSFRKLF
                     KYAMTGIKQFSHVYILVPNLISIFLSIALILQIIFGHIYKYDYRYIYIAITAGVLLLN
                     ITLKYVMKLGYDIRDQLLNRPHYLTAETNIE"
     gene            1117..1785
                     /locus_tag="PK460_03055"
     CDS             1117..1785
                     /locus_tag="PK460_03055"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOE54098.1"
                     /translation="MSKEKKQISKNLVYRVPVYLTVSRRLLKDGARYITAPQISAITG
                     INVETVKKDLSQICKTPGNPKLGREADVLVSEIIEFGVYTKLTNAVIIGAGHLGSALL
                     NYDGFNEWGLQIIAAIDNNPALINTTINDKQIHGVDDLDKIIKEYNVQIAIITVPKEY
                     AQELVDHAVAAGVKAIWNFAPTHISVPKDVVLQNENMASSLSLLNYNLNLKTSKQKRK
                     TKRK"
     gene            complement(1833..3536)
                     /locus_tag="PK460_03060"
     CDS             complement(1833..3536)
                     /locus_tag="PK460_03060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOE54099.1"
                     /translation="MKFSQYPFNVPDVPKIQKQLTKYIEQFKNAKDTNAASRVMRKIS
                     KYVDDFVTDAVIISVKFSQDSRNEEYVKAQEYVDHHFPYLSALMNEYNKLLVASPFRA
                     ELEKKWGSYLFKMIETQLKTFDPSIIELLQKESQLTNEYSKLIASAQIKFDGEVYNIP
                     QMGKFTQDKNRDVRKRASAAVVKFFEENNDELGRIYHELVQVRHAMAVKLGYKNFIEL
                     GYLKLGRLDYTAKEVAGYRDQIYREVVPLAHKLYRTSLRRAGIKSPRHYDLNQSFSDG
                     NPVPLGDSKYLVKAATKMYDELSPETGEFFRFMVDKELLDLDARPGKRGGGYMTYFPR
                     YQAPFIFANFNGTAGDVDTLTHEVGHAYQGYESRHIKVSEYRQPTLEACEIHSMSMEF
                     FAWPWMESMFDDADKYRLSHLEGTIKFLPYGVAVDEFQEGVYANPEMTHEERCAMWRE
                     IEKKYLPIRNYKGAPFLEKGTFWLRQSHIFSSPFYYIDYTLAQVVAFQFATEMWKNRD
                     RAWNKYVKLCRLGGRYPFVELLAKAKLRNPFEDGNVRRVITPLKRKLKELTQVLESKN
                     D"
     gene            complement(3551..5152)
                     /gene="gpmI"
                     /locus_tag="PK460_03065"
     CDS             complement(3551..5152)
                     /gene="gpmI"
                     /locus_tag="PK460_03065"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOE54100.1"
                     /translation="MNKRPIILTILDGYGYSEETKGNAVKKAKTPNINAFYKNNPTVL
                     IEASGEAVGLPAGQMGNSEVGHQNIGAGRIVYQSLTYINQLIKDGRFFENEVFVNAVK
                     HTLKYNSKLHLMGLVSDGGVHSHINHIKAFLDLAKMHGAKEVYLHALMDGRDVAPQAG
                     PEYIQELVDYMAKINFGKLATIGGRYYGMDRDKNLNRTNVHYDAIVNRKGLFFSDFRE
                     YFKEQYETLPKNGFDPSDEFLVPGINKNVNGQLGDNDAVIFFNFRPDRAIQFGTIVTN
                     PYFYQNPPLKDGKPEYKAFVPSPILKNIYYACTMKYADSVHGHIALSLPKLDNGLGVF
                     LADKGYTQLRIAETEKYAHVTFFLDGTVNYDGVEKPELKGARRILVPSPKVATYDLQP
                     EMSAPGVTDALLKELDKGDLDVVILNYANPDMVGHTAIESAVIKALETVDACVGRILE
                     WVEKHDATIMITADHGNSEKLLDEEGKPFTAHTSNPVRFGINRKDIKLMKEGGKLSNI
                     APTIIELLGEVPPSEMTEPSLIIKN"
     gene            complement(5224..5697)
                     /locus_tag="PK460_03070"
     CDS             complement(5224..5697)
                     /locus_tag="PK460_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54101.1"
                     /translation="MQIVRIIILVLVVIYLLLAFVFMHISLDYTRQLKKSKETIHSLF
                     AGQIALFAMIGKELESPNSEAQVMNELLEKREFTELNKLAAEKERAYQELAAKKKDTT
                     PQTAQLLQGLSENVVLIRNEIYRHNKLVDNINVNVDSVIFSLFVVILRLKRLTKI"
     gene            5882..6550
                     /locus_tag="PK460_03075"
     CDS             5882..6550
                     /locus_tag="PK460_03075"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOE54102.1"
                     /translation="MENIVRMPILGERFPELDTMTTFGKKKLPNDYEGKWFILFSHPG
                     DFTPVCTTEFIAFNEEIEEFRKMNAELIGLSIDQLQSHMKWTEFIGKITGKEIGFPII
                     ADELGRVANALGMVHPGKGTNTVRAVFIVDPKGVLRLMMYYPQEVGRAVYEVKRALNA
                     LQVADEHKVAAPENYPNNAFLGKDMVILPPPGTEKQKADRLALLERGEAKSYDWWFSY
                     KKLK"
     gene            6704..7537
                     /locus_tag="PK460_03080"
     CDS             6704..7537
                     /locus_tag="PK460_03080"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE54103.1"
                     /translation="MGTAKPIIAIIYDFDKTLTTTDMQNYNFIPALGMTPEEFWGETG
                     KFSDKTGVERILSYMYMMIVKAREKGINLTKEFLYEQGKNMEYFDGVLTWFDRINEYG
                     EKKSIKIEHYLISSGTKEIIEGSEIAKHFKAIFGCEFYFDPQTKLPVWPKLAINYTAK
                     TQFYFRITKGILDVSEDNALNRKLVDHRIPHRNIIYIGDGMTDVPVMLLVKQNGGHSI
                     AVYPKGEREKVEYLYTDGRVNFVARADYTEKSDINSIVRLIIDQVSIAEQIEKKKQLL
                     N"
     gene            7540..7911
                     /locus_tag="PK460_03085"
     CDS             7540..7911
                     /locus_tag="PK460_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOE54104.1"
                     /translation="MNIYELNEIVEGTITEIRPYGAIITFPENRTGLLHIKQISDAYI
                     SNINTYLQLDAQIKVRIIEIDEHNGFLKLSLKSVPYNERIIFERDKKATNINPAQTDF
                     SALEKALPLWIEKAIKEAQND"
     gene            7904..9181
                     /locus_tag="PK460_03090"
     CDS             7904..9181
                     /locus_tag="PK460_03090"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOE54105.1"
                     /translation="MIRLNYDNVLTTIDFEKYRSKLEKINEGINQKKGAGNDYLGWAD
                     WPLHYDKKEFNLIKETALMIRETFDTLVVTGIGGSYLGARAAIEAINGLLPQKGPEII
                     FLGHTFSPTLTAQTLEYLKTKNFAINVISKSGTTTETSIAFRLLKQLLIEKVGEANAN
                     KAIFATTDAKTGALRKLATQNGYTTFTLPSDIGGRYSVLTAVGLLPLAVAGIDIDDLM
                     AGALKARSESVDNLDHDLYKYAVIRDTLYRQNYKSELYVVYEPQYAMFNEWLKQLYGE
                     SEGKEKKGLFPTSVTFSTDLHSLGQFIQDGSPILFETTLFVENPNQDVVIPSEKENLD
                     ELNYLAGKNLALVNKQAFKGTLAAHVEDGKVPNLVFEIATLDAHTLGYMFYFFMRSCA
                     VSAYLLDINPFNQPGVEVYKKNMFALLGKPSRK"
     gene            9267..9342
                     /locus_tag="PK460_03095"
     tRNA            9267..9342
                     /locus_tag="PK460_03095"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9300..9302,aa:Val,seq:tac)
     gene            9363..9446
                     /locus_tag="PK460_03100"
     tRNA            9363..9446
                     /locus_tag="PK460_03100"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9397..9399,aa:Tyr,seq:gta)
     gene            9456..9531
                     /locus_tag="PK460_03105"
     tRNA            9456..9531
                     /locus_tag="PK460_03105"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9488..9490,aa:Gln,seq:ttg)
     gene            9535..9610
                     /locus_tag="PK460_03110"
     tRNA            9535..9610
                     /locus_tag="PK460_03110"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9568..9570,aa:Lys,seq:ttt)
     gene            9613..9700
                     /locus_tag="PK460_03115"
     tRNA            9613..9700
                     /locus_tag="PK460_03115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9647..9649,aa:Leu,seq:taa)
     gene            9792..10031
                     /locus_tag="PK460_03120"
     CDS             9792..10031
                     /locus_tag="PK460_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOE54106.1"
                     /translation="MEDLKQTENKIKVLLNRIRPYIQSHGGDFEFVRFEEGFVYLKML
                     GACEGCPLVNQTLEYGIEEILMEEVPGVLGVLLDE"
     gene            complement(10208..10858)
                     /locus_tag="PK460_03125"
     CDS             complement(10208..10858)
                     /locus_tag="PK460_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54107.1"
                     /translation="MKRKGSLLILIIPMLFSLTACNSGLNEYRENAKAEIARFVTDWQ
                     HIFNDHQEALNIAVQKGEEAINKARNKKEIDSIVKNTKADIDALLINDDYEFSISTEM
                     VVKKSGEDFSVEVKLKNNSGADIEMSYFWSPFWPFAPRWQYPLFIEPSEFPETVTFLN
                     NETYTETWTLGANFEVGKHDLRFKALFYPNYYEDDTNCVVIWSPHIEIAVDYYLGK"
     gene            complement(10997..>12358)
                     /locus_tag="PK460_03130"
     CDS             complement(10997..>12358)
                     /locus_tag="PK460_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOE54108.1"
                     /translation="TSTEGRTGQKELSFYATNDFHGSIVETNSEPGIFKLGSYLKSEF
                     AAKRNKTVYVDAGDIWQGSADSNINRGKFLVEAMNVLKLDAQTIGNHEFDWYDTVIET
                     NKELANYPFLGANIMVRETGELATNLVSYDETFKASTMVTRNDVNIGIIGTIGMLEGS
                     ILAGAIAPYTFKPVTTYIEREAENLRAQGADMIVLSTHDSLANSLGAYAPIIENKVVD
                     IIFSGHHHVRDNQFINDVPIMQTEGNGKQVMEVHGYYDFNLKEFKVTSREFTEAADIK
                     RDYTEDTELLNLFNEKYAEEIERVKGEVIGSLTNYINKSTLVKLANKVMYNFASITHN
                     PDTIIAVHNYGGVRVDGIDAGEVTYGDIYKAFPFDNEIKIFEDVLGSTASSFIGSNAS
                     YPIGKFDIQNDQEYTIVTIDYVSDSKYSNIRDYPHTVTGEYVRELIAEYFRTNGEIDP
                     YTI"
     assembly_gap    12359..12458
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12589..13371)
                     /locus_tag="PK460_03135"
     CDS             complement(12589..13371)
                     /locus_tag="PK460_03135"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOE54109.1"
                     /translation="MKYKKELNAAIEAGKIARDIVLKIYESDFDVEIKDDNSPVTLAD
                     KMADKAMREYLGPLFPDYAFLTEESDDDLSRLTTDYVWIIDPVDGTKDFIHRDGEFTV
                     CIALTYKGEVVVGVIVVPVLNEIYYATKGGGAYELFADGSTKKLSVNDKTSNLTATRS
                     RHHFKPADNEYIEKHKDLISNVITYGSTLKAIKIAKGEIELFYRSGPGTKEWDIAAAD
                     IIVTEAGGIFIGPDGKKFTYNKADIHNRNGYLIVNRKENIRL"
     gene            complement(13361..14302)
                     /locus_tag="PK460_03140"
     CDS             complement(13361..14302)
                     /locus_tag="PK460_03140"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOE54110.1"
                     /translation="MFKNLRKFIEEHDKIAIFSHIYPDGDAVGSIFGLRELIKTSYPH
                     KEVFGLGSNVQPFVSIVGELDEVSDDFIKESAAIIVDVANGERVEDQRFKLAKASFKI
                     DHHIFVEKFTDEELVLTKRIATAEIIGELMMKEKLRTNKLGATALALGIITDSGRFRY
                     DLTSGNTFSVMARLTNIGADLALINENLAKRSLADFKKRGHFLSNYETYENVIYIIVP
                     YLKLQELDILPSEGSNYVNTYSNLDDYPLWATFFIEKDGRVFVELRSKNYNVQKVATL
                     FGGGGHLKASGCRLENEARIADVLQQLTKAEELKDEV"
     gene            complement(14303..15160)
                     /gene="fba"
                     /locus_tag="PK460_03145"
     CDS             complement(14303..15160)
                     /gene="fba"
                     /locus_tag="PK460_03145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOE54111.1"
                     /translation="MPLVSMKEMLIKANKEKYAVGQFNINNLEWTQAILDEVQELQAP
                     VILGVSEGAAKYMGGYNVVVQMVKALIKDRGYTIPIAIHLDHGSSEEVCKKAIDAGFT
                     SVMIDASHHPFKENLEITKRVVDYAHARGVSVESELGSIGGQEEHVIAETLYADPNEC
                     LEMVQKTKIDCLAPALGSVHGPYKGEPKLGFDEMDHINKLVGVPLVLHGGSGIPDFQI
                     QMAIERGTAKINVNTEIQQEWTKIVRETLANNDKVYDPRKIIGPGMKGIRKVVNEKCR
                     VFGCIGKAA"
     gene            complement(15190..>15585)
                     /gene="rpoE"
                     /locus_tag="PK460_03150"
     CDS             complement(15190..>15585)
                     /gene="rpoE"
                     /locus_tag="PK460_03150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HOE54112.1"
                     /translation="RRYMKKRTNIEIAYTLLKEHGESLSFYDLWQGVVNEHEYSEAEG
                     HDLISRFYTALTMDGRFVNLGDNMWALRENVLFDDIALPLNEVYTEVDEAGDEEEEDE
                     GPEILYFDDETGQADEAKVRSTLDEEDEE"
BASE COUNT         4877 a         2975 c         2609 g         5024 t
ORIGIN      
        1 caatttaacc ttttcttcta ttatgaaaat aataaatttt tacttactat ccataaaatg
       61 atatcatttt attaattaag ggagttattt atgaaaaaga aaataagcct cataattcct
      121 tgttataacg aggaagaaat gattgatatt ctctatgatg aaattaataa agttttagta
      181 aaattaccaa aatatgattt tatctttctt tttgttgatg acggatcaaa agatcaaaca
      241 ctgtcattaa tcaaagcaaa agcaaagaaa gatcatcgcg ttaaatatat ttctttttcg
      301 cgaaactttg gcaaagaatc ttcaatgctt gccggccttg aagccgcaca aaagcttgat
      361 gttgatgcag cgttacttat ggacgctgat atgcaagatc cgccatctct tatgcttgaa
      421 ttttttaaat attatgaaga aggctataaa tatatcttta cccgtaataa gacacgcaaa
      481 ggacaagcgc ttttaaaacg cttcttcgct aacgcatttt atgtgattta tgcttggctt
      541 acaggggaca aaaatagtgt gcatagtgcc cgcgattttg cccttcttga ccgtgatgtt
      601 ataaatgctt tcctgactta taaagataaa catcgttatt ctaaaggaat ctcttcacaa
      661 attggtttta aaaagaaatg cattgaatat gatttccaag aacggcaagc tggcactact
      721 aaatggtcgt ttagaaagct atttaaatat gcgatgacgg ggattaaaca gttttcacat
      781 gtttatattt tagtgcctaa cctcatcagc atcttcttaa gcattgcttt aatcttacaa
      841 attatattcg gccatattta taagtatgac tatcgttata tttatatagc aattaccgct
      901 ggggttttat tgctgaacat tacgcttaaa tatgtcatga agttaggata cgatattcgt
      961 gatcaactct taaatcggcc acattactta actgctgaaa caaatatcga gtaaaaatac
     1021 cgatatgctt atatcaatgt tgttattttt taatgttttc ctttataata aggctgacag
     1081 aaaattttcc cgtaatgcag aaaggtttct ttttttatga gtaaagagaa aaaacaaata
     1141 tccaaaaacc tcgtataccg tgtccctgta tatttaacgg tctcgcgccg tcttttaaaa
     1201 gatggtgccc gctacataac tgcaccgcaa attagtgcga ttaccgggat taatgtcgaa
     1261 acagttaaaa aagatttatc gcaaatttgt aaaactccag gtaacccaaa acttggccgt
     1321 gaagctgatg ttttagtatc tgaaattatt gaatttggtg tgtacacaaa attaactaat
     1381 gccgttatta ttggtgctgg tcatcttggt agtgccttgc ttaattatga tggctttaat
     1441 gaatggggtc tgcaaattat tgccgcaatt gataataatc cagcattaat taacactaca
     1501 attaatgata aacaaattca tggagttgat gatctagata aaatcattaa agagtacaac
     1561 gtacaaatcg ctattatcac cgtaccaaaa gaatatgctc aagagttagt cgatcacgct
     1621 gttgcggcgg gtgttaaagc aatttggaac tttgctccta cacatataag tgtgccaaaa
     1681 gatgttgtct tacaaaatga gaatatggca agtagtctca gtcttttaaa ttacaattta
     1741 aatttaaaaa catcaaaaca aaaaaggaaa acaaaacgga aataataagg aaaagggctg
     1801 caaattgcag cccttttaat aaaatttaag ctttaatcgt ttttgctttc taacacttgt
     1861 gttaactctt ttaatttacg tttaagtgga gttataacac gccgtacatt cccatcttca
     1921 aacggattac gaagtttagc tttcgctagt aattccacaa atggataacg accaccaagg
     1981 cggcaaagtt tcacatactt attccatgca cgatcacgat tcttccacat ttctgttgca
     2041 aattggaaag caacgacttg tgctaaagtg tagtcaatat agtaaaacgg actactaaag
     2101 atatgacttt gacggagcca gaatgtacct ttttctagga aaggagcgcc cttgtaatta
     2161 cgaattggta aatatttctt ttcaatttcc cgccacatgg cacaacgctc ttcatgagtc
     2221 atctctggat tagcgtatac gccttcttgg aattcatcaa cggcaacgcc atagggcaag
     2281 aatttaatcg taccttctaa gtgtgacaag cgatatttat cagcgtcatc gaacatactt
     2341 tccatccatg gccaagcaaa aaattccatg ctcatgctat gaatttcgca agcttcgagt
     2401 gttggttgac gatattcact aactttaata tgacgacttt cgtacccctg ataagcgtgt
     2461 cctacttcat gcgttaaagt gtcgacgtca ccagctgttc cgttaaaatt agcaaagata
     2521 aatggtgctt ggtaacgcgg gaagtaagtc atgtaaccac cacctctttt accagggcga
     2581 gcatctaaat ctaaaagttc tttatcaacc ataaaccgga agaattctcc ggtttcagga
     2641 ctaagttcat catacatttt agttgcggct ttcactaaat atttactgtc acctagtggc
     2701 actgggttac catcgctaaa gctttggttt aagtcataat gacgtggtga cttaatacct
     2761 gcgcggcgta aacttgtgcg atataattta tgtgcaagtg ggacgacttc acgataaatt
     2821 tggtcacgat agcctgcgac ttctttagcg gtatagtcaa gccggcctaa ttttaagtaa
     2881 ccaagttcaa taaagttttt ataaccaagt ttaactgcca ttgcatggcg aacttgcaca
     2941 agttcgtgat aaatgcgacc aagttcatca ttgttttctt caaagaattt aacaacagcg
     3001 gcactagcgc gttttcttac atcacgattc ttatcttgag taaatttacc catctgcggg
     3061 atattgtata cttcaccatc aaacttaatt tgtgcactgg caataagctt tgaatattca
     3121 ttcgtgagtt gtgattcttt ttgtaataat tcaatgattg aaggatcaaa tgttttaagt
     3181 tgagtttcaa tcatcttgaa taagtaactt ccccacttct tttcaagttc agcgcggaag
     3241 ggagaagcaa cgagcagttt attgtactca ttcattaatg cacttaaata cgggaaatga
     3301 tgatcgacat attcttgggc tttcacatat tcttcattgc gtgaatcttg cgagaattta
     3361 acactaataa taacagcatc agtgacaaag tcatcaacat acttagaaat cttgcgcata
     3421 acacgagatg ccgcatttgt gtctttagca tttttaaatt gttcaatata ctttgttaat
     3481 tgcttttgaa tttttggtac gtcagggacg ttaaatggat attgactaaa tttcatattt
     3541 gttttccttt ctaattttta attattaaac ttggttcagt catttcacta ggcgggactt
     3601 ccccaagtaa ttcaatgatt gttggggcaa tattactaag tttgccacct tctttcataa
     3661 gtttgatatc tttacgatta atcccaaagc gtactggatt actcgtatga gcggtaaatg
     3721 gtttaccttc ttcgtcaagt aatttttccg agttaccgtg gtcagctgta atcataatcg
     3781 ttgcatcgtg tttttccacc cattcaagga tgcgcccaac acatgcgtcc acagtttcaa
     3841 gtgctttaat cactgcactt tcaattgcag tatgaccgac catatcaggg tttgcataat
     3901 ttaatataac aacatcaaga tcacctttat ctaattcttt aagtaaagca tccgtgactc
     3961 ctggcgctga catttctggt tgtaagtcat aagtcgctac tttgggggac ggcactagga
     4021 tacgacgcgc acctttaagt tcaggctttt caacaccgtc ataattaacc gtaccatcta
     4081 agaaaaatgt aacatgcgca tatttttctg tttcagcgat tcgtagttgt gtgtaacctt
     4141 tatccgctaa aaagacacct aagccattat caagtttagg tagtgataaa gcgatatgtc
     4201 catgaactga atcggcatat ttcatcgtac atgcgtaata aatatttttt aagattgggc
     4261 ttggcacaaa tgccttatat tctggttttc catcttttag tggtgggttt tgatagaaat
     4321 atggattagt gacaatcgtt ccaaattgaa tcgcacgatc tggacggaaa ttaaagaaaa
     4381 taacagcgtc attatctcct aattggccat tgacattttt attaattccg ggaactaaaa
     4441 attcatcact tggatcaaag ccgtttttag gtaatgtttc gtattgttct ttgaaatatt
     4501 ctctaaaatc actaaagaat aagcccttac gattcacaat cgcatcgtaa tggacattcg
     4561 tacgatttaa attcttatca cgatccattc cgtaataacg gccaccgatg gtagcgagtt
     4621 taccaaaatt aattttcgcc atataatcca ctaattcttg gatatattct gggccagctt
     4681 ggggcgctac atcgcgtcca tccattaaag cgtgtaaata aacctcttta gcgccatgca
     4741 tttttgctaa atctaaaaag gctttaatgt gattaatatg tgaatgaaca ccgccatcac
     4801 taactaaacc cattaaatgt aatttagaat tgtatttaag tgtatgttta acggcattaa
     4861 caaatacttc attttcaaaa aagcgaccat ctttaattag ttgattaata taagttagtg
     4921 attgataaac aatgcggcct gcaccaatat tttggtgacc gacttcacta tttcccattt
     4981 gcccagcggg taaacctacc gcttcgcctg aggcttcaat taggacggtc ggattatttt
     5041 tatagaatgc gttaatgttt ggggtttttg cttttttaac ggcattccct tttgtttctt
     5101 cgctatagcc gtagccatct aagattgtaa gtataatagg acgtttattc ataataaaaa
     5161 acctctttcc gatagtaatt atattactat aaagggtaat tgtaagcaaa aatgcttatt
     5221 taactagatt ttagtaagtc tttttaatcg taaaataacg acaaacaatg aaaatataac
     5281 agaatcaaca ttaacattaa tgttatccac tagtttgtta tgacgatata tttcattacg
     5341 aataagtaca acattctcac tgagaccttg tagtagttgc gctgtttgtg gagttgtatc
     5401 tttctttttc gctgctaact cttgataagc gcgctctttt tccgccgcta gcttattaag
     5461 ttcggtaaat tcacgttttt ccaaaagttc attcattact tgtgcttcac tatttggtga
     5521 ctctaattct ttaccaatca ttgcaaaaag ggcaatttga cctgcaaaaa ggctgtgaat
     5581 tgtttctttg ctctttttta attgtcgtgt gtagtcgagt gaaatatgca taaaaacaaa
     5641 tgctaagagc aagtatataa cgactagaac caaaataata atacgcacga tttgcatagt
     5701 ttaattataa catacaacga ttattgtaaa ttagtgattt tatttaagat taattatgaa
     5761 agcgttatat taactttttt caagaataaa aatagcaact taccaaaaga aagcatattt
     5821 aaagaagttt ccattaaaaa aagataaaat atagctgtat acaaaaggag gaaattaata
     5881 tatggaaaat attgttagaa tgccaattct tggcgaacgc ttccccgagt tagatacaat
     5941 gacaactttc gggaaaaaga aattacctaa tgattacgaa gggaaatggt ttattctctt
     6001 ctcacaccca ggtgacttca caccagtctg tacgactgaa tttattgctt ttaatgaaga
     6061 aattgaagaa tttagaaaaa tgaatgcgga acttatcgga ctttcaattg accaacttca
     6121 atcacatatg aagtggactg aatttatcgg taagattact ggcaaagaaa ttggattccc
     6181 aattatcgct gacgaactag gccgtgtcgc taatgcgctt ggtatggttc accctggtaa
     6241 aggcacaaat actgttcgtg ctgtcttcat cgttgatcca aaaggtgttt tacgcttaat
     6301 gatgtactac ccacaagaag ttggccgtgc agtttacgaa gtaaaaagag cacttaatgc
     6361 gctccaagtc gctgatgaac ataaagttgc tgctccagaa aactacccaa ataatgcttt
     6421 cttaggcaaa gacatggtca tcttaccgcc accaggaacc gaaaaacaaa aagcagatcg
     6481 tcttgcgtta cttgaacgcg gtgaagcaaa gagttatgac tggtggttct cttataaaaa
     6541 attaaaataa gaattcctat aactactaaa caaggtgcta caaaacgcac cttgtttttg
     6601 tttataatac ttttcaacga tttagtggct atttataagt ccttattcgt tttgacaatc
     6661 actctattga gtgatattat gttataaatg gaggtttttc aacatgggta cagctaaacc
     6721 aattattgct attatctatg actttgacaa aacattaacc accacagata tgcaaaacta
     6781 taattttatt cctgcgcttg gtatgacacc agaagaattt tggggtgaaa caggaaaatt
     6841 tagtgataaa actggcgttg aacggatttt atcatatatg tatatgatga ttgtcaaagc
     6901 tcgtgaaaaa ggtattaatt taacgaaaga atttctttat gaacaaggca aaaacatgga
     6961 atacttcgat ggggtcttaa cttggtttga ccgcattaat gaatatggtg agaaaaaaag
     7021 cattaaaatt gaacactatc tcattagcag cggcacaaaa gaaatcattg aaggttcaga
     7081 aattgctaaa cactttaaag ccatttttgg ttgtgaattt tactttgatc cacaaactaa
     7141 attaccagta tggccaaaac tagcgattaa ctacaccgct aaaacacagt tctacttccg
     7201 cattactaaa ggtatcttag atgtcagcga agataatgcc ttaaaccgta aactagtaga
     7261 ccatcgcatc ccacatcgca acattattta tatcggcgac gggatgacag atgttccggt
     7321 aatgctcctt gttaaacaaa atggcggtca ctcaattgct gtttatccaa agggtgaacg
     7381 cgaaaaagtt gagtacttat acactgatgg ccgggtaaac tttgttgctc gcgctgatta
     7441 tacagaaaaa agtgatatta attccattgt tcgcttaatt attgaccaag tgtctatcgc
     7501 cgaacaaatt gaaaagaaga aacaacttct taactagtta tgaacattta cgaactaaat
     7561 gaaattgtcg aaggcactat tactgaaatt agaccatatg gtgccattat tacctttcct
     7621 gaaaatagga ctgggctttt acatataaaa caaattagtg atgcctacat tagtaacatc
     7681 aacacatact tgcaacttga tgcacaaatt aaagtgcgga taattgaaat tgatgaacat
     7741 aatggtttct taaaactttc acttaaatcc gttccttaca atgaacgcat tatttttgaa
     7801 cgcgataaaa aagcaacaaa cattaatccg gcacaaactg attttagtgc acttgaaaaa
     7861 gcattgccgc tttggattga aaaggctatt aaggaggcgc aaaatgatta gattaaatta
     7921 tgacaatgta ttaactacga ttgattttga aaaataccgc tctaaacttg aaaaaattaa
     7981 tgaaggcatt aatcaaaaaa agggagcagg aaatgattat cttggttggg ctgattggcc
     8041 attacattac gataaaaaag aatttaattt aattaaagaa acagccttaa tgattagaga
     8101 aacctttgat actttagtcg ttactggtat tggtggttct tacttggggg caagagctgc
     8161 aattgaagca attaacggat tattgccgca aaaaggacct gaaattatct ttttgggtca
     8221 tactttttct ccgaccttaa cagcgcaaac gctcgaatac ttaaaaacaa aaaactttgc
     8281 gattaatgtt atttctaaaa gtggtaccac tactgaaaca agtattgctt ttcgcttatt
     8341 aaagcaacta ttaatcgaaa aagtcggtga agcaaatgct aataaggcaa tttttgcgac
     8401 tacagacgct aaaactggtg cgctccgtaa actagcgaca caaaatggct acactacttt
     8461 tacattgcca agcgatatcg gtggtcgtta tagtgtttta accgctgttg gacttctacc
     8521 acttgctgtt gcgggaattg atattgatga cttaatggcg ggagctctta aagcacgcag
     8581 cgaaagcgtc gataatttag atcatgacct ttataaatat gcagttattc gcgacacact
     8641 ctatcgtcaa aattacaaga gcgagctcta tgttgtttac gaaccacaat acgctatgtt
     8701 taatgagtgg ttaaaacaac tatatggcga aagcgaaggt aaagaaaaga aaggtttatt
     8761 cccaacttca gttaccttct ccactgattt acatagctta ggacaattta ttcaagacgg
     8821 atctcctatt ttatttgaga caacgctctt tgttgaaaat ccaaaccaag atgtcgtcat
     8881 tcctagtgaa aaagaaaact tagatgaatt aaattacctt gcgggaaaaa accttgccct
     8941 tgtcaataag caagctttta aaggcacact cgccgcccat gttgaagatg gtaaggttcc
     9001 taatcttgtc tttgaaattg ctacacttga tgcccacact cttggttata tgttctactt
     9061 ctttatgcgc tcatgtgccg taagtgctta tctacttgat ataaatcctt ttaatcaacc
     9121 aggcgtcgaa gtttataaaa agaatatgtt tgccctactt ggtaaaccta gcagaaaata
     9181 ataacaggcc gttttgatag tattatttgt tgactaaata actatctaat gatatattaa
     9241 gtaagcataa attagcgacc agtcccggat ggttagctca gctgggagag catcgccttt
     9301 acacggcgga ggtcgggggt tcaaacccct caccatccac catttatttt atgccaaaag
     9361 tcgggagagt agcgaagtgg tcaaacgcgg ctgactgtaa atcagttcct gagggttcgg
     9421 tggttcaaat ccgccctctc ccaccatctt ttgtttgggg tatagccaag cggtaaggca
     9481 acagactttg actctgtcat ctccctagtt cgaatctagg taccccagcc attcctcccg
     9541 ttagctcagg cggtagagca tttgactttt aatcaaaggg tcgtaagttc gattcttaca
     9601 cgggagacca tcgcccgggt ggcggaacag gtagacgcaa gggacttaaa atccctcggt
     9661 tgttgagacc gtgccggttc aagtccggcc ccgggcacca gttccaaaaa aattgtccta
     9721 ctacggtagg gcttttttat tcataagcat tctttttagt aaatataatt tcaatttgtt
     9781 atactatttg catggaagat ttaaaacaaa ctgaaaacaa gattaaagta ctacttaacc
     9841 gcatcagacc ttatattcaa agtcacggtg gtgattttga atttgtaaga tttgaagaag
     9901 gttttgttta tttaaagatg cttggcgctt gcgaaggctg cccacttgtt aatcaaacgc
     9961 tagaatatgg tatcgaagaa atattgatgg aagaggtacc aggcgttctt ggcgtcttac
    10021 ttgatgaata aaagctagta caaaaagaaa aggtgcactt aagatgcacc ttttttacat
    10081 tattccttac tatttataat aaagttttgt gtcacttgtc ttaaaatcta ctgctatcag
    10141 tgtagtctaa cagccactta aggagaaaag tatccctaca aatagtacgc gttttataaa
    10201 gcctctttta cttgcctaaa taataatcca ctgctatttc aatatgcggc gaccatatca
    10261 ccacacaatt ggtatcatct tcataataat taggatagaa aagcgccttg aatcgtaagt
    10321 catgctttcc gacttcaaaa ttagcgccta aagtccatgt ttccgtgtat gtttcattat
    10381 ttaagaaagt aactgtttca ggaaattcac taggctcaat aaaaagtggg tattgccatc
    10441 taggggcaaa gggccaaaat ggtgaccaaa aatatgacat ttcaatatct gcgccactat
    10501 tattttttaa tttaacttca acactaaaat cttcaccgct ttttttaaca accatctctg
    10561 ttgatatact aaattcataa tcatcattta ttaaaagagc atcaatatcc gcttttgtat
    10621 tttttacaat actgtcaatt tcttttttat tccttgcttt attaatagct tcttcccctt
    10681 tttgtacagc tatatttaaa gcttcttgat gatcattaaa gatatgctgc caatcagtga
    10741 cgaaacgcgc tatttcagct ttagcatttt cgcgatattc gttaagaccg ctattacacg
    10801 cagttagact aaaaagcatt ggaattataa gaataagtaa acttcctttt cgtttcataa
    10861 gatgtcacag ccttttattt tagttataat ttgcggcaat attaaagttt tatctctacc
    10921 cttattttat tacaaggtgg ggaatttaca atacgaaaaa agctataaag tattctaata
    10981 aaattaattt atctatttaa atagtgtaag ggtctatttc accgttagtg cggaaatatt
    11041 cagcaattaa ttcacgaaca tattcaccag taacagtatg tgggtaatca cgaatatttg
    11101 aatacttgct atcactaaca taatcgatag taacgattgt atattcttga tcattttgaa
    11161 tatcaaattt accgattgga taagaagcat tgctaccaat gaaacttgaa gcagttgagc
    11221 ctaaaacatc ttcaaatatc ttaatttcat tatcaaacgg gaaggcttta taaatatcgc
    11281 catatgttac ttctcctgca tcaatcccgt ctactcgcac accaccataa ttatggacag
    11341 caataattgt atcagggtta tgcgtaatac tcgcgaagtt atacatcacc ttattagcga
    11401 gttttacaag tgtactctta ttaatataat ttgtcagtga acctataact tcacctttta
    11461 ctctttcaat ttcttcagca tacttttcat taaataagtt taataattca gtgtcttctg
    11521 tgtaatctct tttaatgtcc gctgcttcgg taaattcacg gctagtaact ttaaattctt
    11581 ttaagttaaa gtcataatag ccatgcactt ccattacttg tttaccatta ccttccgttt
    11641 gcataattgg cacatcatta ataaactgat tatcacgcac atgatggtga ccggaaaaaa
    11701 taatatccac gactttattt tcgataatag gcgcataagc accaagacta ttagcgagtg
    11761 agtcatgcgt tgataagaca atcatatctg cgccttgtgc tcgtaaattt tctgcttcgc
    11821 gttcaatata agttgttact ggtttaaatg tgtaaggggc aatagcacct gctaaaatgg
    11881 agccttcaag catcccaatc gtcccaataa tcccaatgtt aacatcgttt cttgttacca
    11941 tcgtactagc tttaaaagtt tcatcgtatg acactaaatt cgtggcaagc tcaccggttt
    12001 cacgcaccat gatattagcg cctaaaaacg gataattagc gagttcctta ttcgtttcaa
    12061 taacagtgtc ataccaatca aattcgtggt taccaatagt ttgcgcatca agctttaaaa
    12121 cattcattgc ttcaactaaa aacttaccac gattaatatt tgaatcagca ctgccttgcc
    12181 agatgtcacc agcatcaaca tacactgttt tatttctttt agcagcgaat tcacttttta
    12241 aatatgaacc aagtttaaaa ataccaggct cactatttgt ttcaacaatt gacccatgaa
    12301 aatcattagt agcgtaaaaa cttaattcct tttgcccagt tctaccttca gtgcttgtnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gctagtaggt tcttcactac
    12481 ctaaggtagg tctagaattg tcacttagag attcacttcc tctttcacat ccgcttacta
    12541 caagcgtaaa aataaggagc ggtatcatta aaataattgt ttttttcttc ataatcgtat
    12601 attttccttc cgattaacga ttaaataacc gttacgattg tgtatatcag ctttattata
    12661 agtaaacttc ttaccatcag gtccaataaa aataccgcca gcttcagtca caatgatatc
    12721 cgctgcagcg atatcccatt ctttagtgcc tggaccacta cgataaaata attcaatttc
    12781 acctttagca atcttaatcg ctttaagtgt ggagccgtat gttattacat tactaattaa
    12841 atctttatgt ttttcgatat attcattatc agcaggttta aaatgatgac gagaacgagt
    12901 cgcggttaag ttactagttt tatcattaac acttaacttc tttgtcgaac catcagcgaa
    12961 tagttcgtag gctcctccac ctttagtagc gtaatatatt tcgttaagta ccggcacaac
    13021 aatgacaccg acaacaactt cacctttata agttaaagcg atacacacag taaattcacc
    13081 atcacgatga ataaaatctt ttgtgccgtc gaccggatca ataatccaca cataatccgt
    13141 ggtaagccga gataaatcat catcactttc ttcagttaaa aaagcataat caggaaaaag
    13201 tggtccaaga tactcacgca ttgctttatc cgccatttta tcggctaaag taacaggaga
    13261 attatcatct ttaatttcaa catcaaagtc actttcataa atttttaaaa caatatcacg
    13321 cgctattttc ccggcttcaa tcgccgcatt taattccttt ttatacttca tcttttaact
    13381 cctcagcttt agtaagttgt tgcaaaacgt cggcaattcg tgcttcattt tctaagcgac
    13441 agccacttgc ttttaagtga ccaccgccac caaaaagcgt agcgaccttt tgcacattat
    13501 aatttttact acgtaattca acaaacacac gcccgtcttt ttcgataaaa aaagttgccc
    13561 ataacggata gtcatctaga tttgaataag tattgacata attactacct tcacttggta
    13621 atatgtctaa ttcttgtagt tttaagtatg ggacaataat ataaattaca ttttcataag
    13681 tttcataatt tgataaaaag tgtccgcgtt ttttaaagtc agcgagtgaa cgcttcgcta
    13741 aattctcatt aattaacgct aaatccgcac caatattagt gagtcgcgcc atcacactaa
    13801 aagtgttacc gcttgttaag tcgtatctaa aacgacctga atcggtaata atcccaagcg
    13861 ctaaggcagt cgcaccaagt ttatttgtgc gaagcttttc tttcatcatt aattcaccaa
    13921 taatttcggc agtagcaata cgctttgtta acactaactc ttcatcagta aacttttcca
    13981 cgaaaatatg atgatcaatt ttaaatgatg cttttgctaa tttaaagcgt tgatcctcaa
    14041 ctctctcacc gttagcgaca tcgacaataa tcgccgcgct ttctttaatg aagtcgtcac
    14101 ttacttcatc taactcgccc acaatagaaa caaagggttg gacattactg cccaacccaa
    14161 aaacttcttt atgcggataa cttgttttta ttaattcacg caagccaaaa atactcccaa
    14221 ctgcatcgcc atcaggataa atgtgtgaaa agattgcaat cttatcatgc tcttcaatga
    14281 acttacgtaa atttttaaac acttatgcag ctttccctat acaaccaaag acgcggcact
    14341 tttcgttaac aactttacga atgcctttca ttcccggacc aataatttta cgtggatcat
    14401 aaactttatc attattcgct aaagtttcac ggacaatctt tgtccattct tgttgaattt
    14461 cagtgttgac attaatttta gcggtaccac gttcaatcgc catttgaatt tggaaatcag
    14521 gaataccact gccaccatgt agcacaagtg ggactccgac taatttatta atatgatcca
    14581 tttcatcaaa accaagtttt ggttcgcctt tatacggacc atgtacacta ccaagcgctg
    14641 gcgctaagca gtcaattttt gttttttgca ccatttctaa acattcgttt ggatcagcat
    14701 ataatgtttc agcaataacg tgttcttctt gaccaccgat gctaccaagt tctgattcaa
    14761 cactaacgcc gcgtgcatgt gcgtaatcaa ctacgcgttt agtaatttct aaattttctt
    14821 taaatgggtg atgacttgcg tcaatcatca cggaagtaaa gccagcatca attgcctttt
    14881 tacatacttc ttcactgctg ccgtgatcta agtggatggc aattggaatc gtatagccac
    14941 gatctttaat taaggctttt accatttgca cgacgacatt ataaccgccc atgtatttag
    15001 cggctccttc actaactcct aaaataactg gagcttgaag ctcttgtacc tcatcgagaa
    15061 ttgcttgggt ccattctaag ttattaatgt tgaattgacc aacagcgtac ttctctttgt
    15121 ttgctttgat aagcatttct ttcatactca ctaatggcat aaaaattctc ctttatcttt
    15181 taaaattatt tattcttcgt cttcttcgtc aagtgtgctt cttactttcg cttcatctgc
    15241 ttgacctgtt tcatcatcaa agtaaaggat ttctggtcct tcgtcttctt cttcctcatc
    15301 acctgcttca tcaacttcag tgtaaacttc gtttagtggt aaagcaatgt catcaaataa
    15361 gacattttca cgtaatgccc acatattgtc gcctaaatta acaaagcgac catccatcgt
    15421 taaggcagtg tagaagcgtg aaattaaatc atgaccttca gcttcactat attcatgttc
    15481 gttaacaaca ccttgccata aatcataaaa tgatagtgat tcaccgtgtt ccttaagtag
    15541 ggtgtaagca atttcaatgt ttgtcctttt cttcatatat cttct
//
LOCUS       DAOSLP010000023        15013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_4110_length_15013_cov_11.221353, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000023 DAOSLP010000000
VERSION     DAOSLP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 15013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15013
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4110_length_15013_cov_11.221353"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..134)
                     /locus_tag="PK460_03155"
     tRNA            complement(59..134)
                     /locus_tag="PK460_03155"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Ala,seq:cgc)
     gene            273..1523
                     /locus_tag="PK460_03160"
     CDS             273..1523
                     /locus_tag="PK460_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage repair protein"
                     /protein_id="HOE54113.1"
                     /translation="MEKVYAVIDLKSFYACVECAERGLDPFTTPLVVCDERRGDGTII
                     MAVTPYLKAKGLSNVMRKFELPRGIPYIFAKPRMELYVQKSTEFNALLLNYFAKEDWY
                     PYSIDETFIHLTPYLELYKKTPEELIEFIIKEIYDHLKLYVTVGIAPNMFLAKCALDL
                     EAKKDPRYYARWTMEDVKTKLWQIKPLHKIWGIGKRYERRLHKLGLFTMGDIAKCPKA
                     KLKEEFGIMGEELWEHANGIDNTDLSEKYVPLDKSLTSGQVLFRDYEVNEAPVLIREM
                     TDELCMRMRKNNLKAKKVGLSIMTSKPESSTYSKSMSLLLPTCSNTELFRAFMEVFNK
                     FPLEGAKIRSLHLSVSGLSSDPYEQLSLIEDYVTQNTKEQRMWETLDELHARYGKDKI
                     MRANALTRASTYLRRTKQIGGHNK"
     gene            1520..1825
                     /locus_tag="PK460_03165"
     CDS             1520..1825
                     /locus_tag="PK460_03165"
                     /inference="COORDINATES: protein motif:HMM:NF020438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HOE54114.1"
                     /translation="MSRGMKKFLPFASLKEQALFLAEMQQQKKRVEKPTLSEDQCAEI
                     NRVLSEYTGETVLIYYYNCGFIKTHQAKIKKIDTLYKRLVFTDLTVSFTNLLHIEII"
     gene            complement(1826..3163)
                     /locus_tag="PK460_03170"
     CDS             complement(1826..3163)
                     /locus_tag="PK460_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54115.1"
                     /translation="MAKNKRKCRPLCFILKAVFVLLGVVVLVLGGYIGYVSLSYYRIG
                     DTSLQVLNNQTAKLSVADIGTKEFKITTYNVGFGAYDRDYSFFMDTSVFKDEYIHSQG
                     NKSNTGDRARGTSKAQVLRNINGAYDTIAALGDLDFMLYQEVDTSSTKSYKVNQYEIG
                     NKKHVDYATIYGINYHSAYLMYPLHEPIGKSNSGISTYSKYHIAKAERREFEITDAFF
                     GKFFDLDRAFTISEIPIEGSVKKLYVFNVHMSAYDKGGVIRKVQLEQLKAAIRVARDY
                     DGDNNYVVVGGDFNHDLVLDNPLVNEEYKANIFNKQETEILEANWYNYFRLDSGKHGT
                     RVPNIITGLEEDYTHDFYDMALSSHGPVNIATCRDASIKFIDKNNNGIVDNAMVTIDG
                     FLVSDNIAVAEIKTIGSGPGLQKENLPESDPRHGLGFVYSDHNPVLMSFKLQA"
     gene            complement(3163..4389)
                     /locus_tag="PK460_03175"
     CDS             complement(3163..4389)
                     /locus_tag="PK460_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOE54116.1"
                     /translation="MKKSDLKKYARLIVRVGANVQKGQDVIITAELEQREFVLMLIEE
                     AYRAKANRVTLDWIDDDISKLHNKYRTVKSMSHFEKWELERYQYYVDKNPVRIYITSE
                     DPDGEKGINQEKRAQVMQNIYPVIKPYRDEMENKYQWTIAGAAGTKWAKKLFPDLTAK
                     QARNKLWDAILTTSRAYGDPIANWNEHNRSMSEKSRKLNELGLISLHYTSKNGTNFKV
                     GLIPNALFLAGGETTLGSNIFYNPNIPTEECFTTPKRGEAEGKVVATMPLSYQGELID
                     GFWFEFKDGKVVDFGAKQNEELLKQMLDMDEGARYLGEVALVPKESPINQLGLLFYST
                     LYDENASCHLALGLGFSNCIRDFEKYSLEELREMGINNSMIHVDFMIGVDDLRIVGTT
                     KYGQEVVIFNEGTWAI"
     gene            complement(4394..4990)
                     /locus_tag="PK460_03180"
     CDS             complement(4394..4990)
                     /locus_tag="PK460_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54117.1"
                     /translation="MEFKIENSVVYGLERAVKASGNPMRTEIEGGALHEKDIKRAVLL
                     GTTKGGEGHDNFLKGILVHVDITAPFYWWKEAQRYHWFDFISSQSTMHMLLKFKIDER
                     CVPETDKRVFPILEELVNDYNAISEEDKDAKVKAWRKIVATLPSGFCLAATMTTNYQQ
                     LKTMYFQRRNHKLKEWHTFCDWCLTLPHFKEFVIKEGK"
     gene            complement(4980..5777)
                     /locus_tag="PK460_03185"
     CDS             complement(4980..5777)
                     /locus_tag="PK460_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOE54118.1"
                     /translation="MDIKTYVLKRKQNLKERILKDGGTPSLVIIQVNDDFASNKYVAG
                     KINDAREVGISAKLIKMPETTRLDELLKKIKKLNKDPHVNGIIVQLPLPKHIDENIVK
                     EAINPAKDVDGFHPLSRFKACTPLGIINYLKDEGVEIRGKNVVIIGRSNIVGKPAAML
                     FLEEDGNVTVLHSKTSREDLKNYVANADIMVVAVGRKHFINDEHTLKNSAVLVDVGIN
                     RDEEGKLTGDIKPGRDVYLQTPVPGGVGLLTRLTLLENVMEAYLHGI"
     gene            complement(5934..6365)
                     /locus_tag="PK460_03190"
     CDS             complement(5934..6365)
                     /locus_tag="PK460_03190"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HOE54119.1"
                     /translation="MNSKSAEQTYRMMHNHIKENPNDKCFLVEVIAQQSRNIVWSVKL
                     DNEPCYDERIRRVSIDEFYKIVTGEENAFYKFCVQLPKTIKKLLANRKDNVKGTNTVI
                     TELQNRGGNLQKAIFFLAFANYCGFTTVEDQIDDLLNGNHH"
     gene            complement(6362..6748)
                     /locus_tag="PK460_03195"
     CDS             complement(6362..6748)
                     /locus_tag="PK460_03195"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HOE54120.1"
                     /translation="MTIDEKVNDDYTLTFITQENFEKHVNELIDKYYEILSDYDLKRF
                     NSNLIDPIKLSIDKYLLDRTWKEIIDTEINRQRDKTITNALGDFHQNIFKYIDRCEVP
                     KTGFDIIYTNEAGQKIYVELKINIIQ"
     gene            complement(6748..7971)
                     /gene="dcm"
                     /locus_tag="PK460_03200"
     CDS             complement(6748..7971)
                     /gene="dcm"
                     /locus_tag="PK460_03200"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOE54121.1"
                     /translation="MIQIDYPKSLHKLRRTLNVSQQQLANILNVSFSSVNRWENGHYE
                     PTIIAKVKLDELFDQVKIKPIAKQEKYKSIELFAGAGGLALGLEQAGFDHIGLVEFEK
                     NAVDTLKINRPEWNVLLEDIREVCERDLEQEFNIKKGELDLISGGAPCQSFSYAGNRL
                     GLEDVRGTMFYYYATFLRKLQPKLFLFENVRGLLSHDKGRTYKTILDIFEEQGYITLY
                     EVLNAWDYSVPQKRERLITIGIRKDLFEEGIFEFPKKHKYKPTIKDVKLDVNPDKEHC
                     KRYSKYKEEIFKLVPPGGYWRDIEPETAKEYMKSCWDMGGGRTGILRRLSLDEPSLTV
                     LTSPFMKQTDRCHPLEVRPFSYRENARFQTFPDDWVFSGTLSSKYKLIGNAVPVNLAK
                     EIGLELIKTLERINE"
     gene            complement(8175..8402)
                     /gene="secG"
                     /locus_tag="PK460_03205"
     CDS             complement(8175..8402)
                     /gene="secG"
                     /locus_tag="PK460_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOE54122.1"
                     /translation="MGTLDIIFIIVSILLIIISLMQGGKSEGASGAITGGGLNIFVKT
                     KERGSEKVISYITYGLAILFFLLAILVKALG"
     gene            complement(8480..10468)
                     /gene="uvrB"
                     /locus_tag="PK460_03210"
     CDS             complement(8480..10468)
                     /gene="uvrB"
                     /locus_tag="PK460_03210"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOE54123.1"
                     /translation="MKKTKHKFVLETTFTPTGDQPTAIKELVAGLERGDKHQVLLGAT
                     GTGKTFTIANVIEKVQKPTLILVHNKTLAGQLYAEFKALFPNNRVEYFVSNFDFYQPE
                     AYVPSRDMYIEKEAVQNAEIEMLRSAAINSLLERNDTIIVASVAAIYGLSDPEEYSKL
                     SFNIVEGEVINRKEFLIKLVDAQYTRNDYELRAGTFRVRGDVIEIMLPISEDYLIQVD
                     TFDDTIEKIREIDVLTGEVRKTFNTYPIFPAYAYASSRERINAIVPKILSDLASRLSY
                     FADEGKLLEAERLEFRTRQDVDSLQEFGICPGIENYARYIDGRKAGEKPYTLMDYFPK
                     DYLLVIDESHVTLPQVRGMFNGDRSRKQNLVDYGFRLPSALDNRPLQFNEFEEMINQV
                     IYTSATPGDYELDKTGGVVVEQLIRPTGLVDPEVFIKPSVGQIDDLMYELTKVIERGE
                     RAMVVTLTIKMAEDLTHYLKEHDFKVVYLHNETKTLERSQIIYELRKGKYDVLVGINL
                     LREGLDIPEVSLVAIIDADKEGFLRSERSLIQVMGRASRNVNGKVIMYANNMTGSMER
                     AIAETKRRRDLQIAYNKKHGIVPTTIIKPLGEPLHNFGTERELERINKGLMSRKQLEK
                     YIREIRAKMNAAAKAYDFEKAIEYREILFELEGKSKKT"
     gene            complement(10449..11420)
                     /locus_tag="PK460_03215"
     CDS             complement(10449..11420)
                     /locus_tag="PK460_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOE54124.1"
                     /translation="MAKQRIKDIFKEDWRTLLKNYSLIVVGSILLAFGTGVFLLPATL
                     NTGGLSGVGIIGEGLFGFDADLVVLVMTWAFFFISLLFLGWRFTIKSLVSSFVYPLAL
                     ILLMRLPAIATETVKLFGEGADVATKLIAGVFGGVFNGVGVALTFRGGGSTGGVDILV
                     VILNKYLRLSYSVMSFVIDGLIIAVGLFVLKDVVLSLIGILSAFVFAITLEFVFVGKK
                     HAMTASIISREKSAEINDFIQNEIGQGTTLVPVLGGYQKDEYMMVVVHFDRSNYARLI
                     NGIAKIDKKAFVSIEGSTVVLGGNFESLNPVKEGLVNIKKKDEKDKA"
     gene            complement(11413..12523)
                     /gene="prfB"
                     /locus_tag="PK460_03220"
     CDS             complement(join(11413..12450,12452..12523))
                     /gene="prfB"
                     /locus_tag="PK460_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_132025324.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOE54125.1"
                     /translation="MEYKVALSKANELKRLLGKLSDSLDVASLKETIDKLTIEINNPD
                     FWNNQNKAKTIIKKLNAAKEKINIYSLVETTVESAFELLKDDTFAEDSEYLSDLAFEI
                     DNAQKEGELLINLLLFSGEYDENDATVSIHAGAGGTESHDWADMLYHMYARFCERQGF
                     SLKVVDYQAGNEAGIKSVTLIIEGNNAYGHLRSEKGVHRLVRISPFDANKRRHTSFAS
                     VNVVPIFDETGDIEINDSDLRVDTYRSGGAGGQNVNKVETAVRITHVPTGIVVSCQME
                     RSQLMNREIAMRMLKSELYQLELERQKAKVSAIEGEKKMIEWGSQIRSYTFAPYTLIK
                     DHRTNYEVGDIARVMDGDIAEFMYKYLEMEARSNG"
     gene            complement(12527..14953)
                     /gene="secA"
                     /locus_tag="PK460_03225"
     CDS             complement(12527..14953)
                     /gene="secA"
                     /locus_tag="PK460_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOE54126.1"
                     /translation="MSFIEKIFRFEQRRLKKIEKTAKKVIAYEKTMEALTDDELKAKT
                     PEFRERLTNGETLDDLLPEAFAVAREAARRTLGQFPYPVQIMGAIVLHHGDVAEMRTG
                     EGKTLTATMAVYLNALSGKGVHVVTVNEYLAARDADWMGQIYRFLGLTVGVNLREKTA
                     REKREEYLCDITYTTNSEVGFDYLRDNMASSVDGRVLRGLEYAIVDEADSILIDESRT
                     PLIISGGAKVQASSYNVADRFVKTLRRDKHFVVDIKTKTCNLTDEGNKLAERMFGVNN
                     IYDPQYSDLVHRIHNALKANYVMSRDVEYMVDANREIQLIDQFTGRIMPGREYSDGLH
                     QAIQAKENVTIKEETVTMATITYQNFFRLYTKLSGMTGTAKTEEEEFRKIYNMRVIQI
                     PTNRPVIREDAVDRVYARKEDKLNALVEEVKERHAKGQPILIGTSSVESSEEVSELLT
                     ANGLKHEVLNAKNHAREADIVALAGNKGQITIATNMAGRGTDIKINDEVKALGGLAVL
                     GTERHEARRIDNQLRGRSGRQGDPGFSRFYVSFDDLLMRRFSSDMMLKLVEGMGNEAL
                     ENKMFTNAITSAQKRIEGQNFDIRKSLLDYDNVLAKQRSIVYKKRDSIIYAGDIKDLV
                     QEQFTLAGLYLAKKAVPETGKDTLVNGVMLKQIMEPNYLPEGSINPSAYDESPPEEIG
                     EDLMLLMYRRYQLRRREWSEELADRVEREITLRAIDRNWTRHIDTMAHLREGIHLRSY
                     ANTNPLQDYVNEGYKLFGEAMDIIAVETVLNLLNAKINVAPPVEEGQEGTEENKETAE
                     KENIKKEDKK"
BASE COUNT         4549 a         3073 c         2346 g         5045 t
ORIGIN      
        1 tttttgttat taataaataa aaaaatagct ccctgagcgg aagctaatac ttttactttg
       61 gtggagccga cgaggatcga actcgctacc tcatcgttgc gaacgatgcg ctctcccaga
      121 tgagctacgg ccccaagtct aactaaaagt ttatcaaact agtaaataat tgcaagggct
      181 atcgcttatc aattttaatg gtgaaaatct tttactaact ttgttcatcc ttctgtctaa
      241 aaattaaagt gtcctcttgt ataattatgg taatggaaaa agtatatgcc gttatcgatt
      301 taaaaagttt ctacgcatgc gtagaatgtg cagagcgtgg tcttgatccc tttaccactc
      361 ctttagtggt gtgcgatgag cggcgcggag acggcactat tattatggct gttaccccgt
      421 acttaaaagc aaaaggttta agtaatgtaa tgcggaagtt tgaacttccg agaggtatcc
      481 cttatatctt tgctaaaccg cgtatggaac tatatgtaca aaaatcaact gaatttaatg
      541 ccctcctact taattatttt gcaaaggaag actggtatcc ttattcgatt gatgaaactt
      601 ttattcattt aacaccgtac ttagaacttt ataagaaaac accagaagaa cttattgaat
      661 ttattattaa agaaatatat gatcacttaa aactctatgt caccgtcggt atagcgccaa
      721 atatgttttt agctaaatgc gcacttgatt tagaagccaa aaaagacccg cgctattacg
      781 caagatggac aatggaagat gttaaaacta aattgtggca aattaaacca ctgcataaaa
      841 tatggggtat tggtaaacgc tatgaaagaa gactccataa attagggctt tttacaatgg
      901 gggacattgc taagtgtccg aaagcgaagt taaaagaaga atttgggatt atgggtgaag
      961 agctttggga acacgctaac gggattgata acaccgactt aagtgaaaag tatgtcccac
     1021 ttgataagtc actaacaagt ggacaagtgt tattccgtga ttacgaagtt aacgaagcac
     1081 ccgttttaat tcgtgaaatg actgatgaat tatgtatgcg catgcgtaaa aataatttaa
     1141 aagctaaaaa agttggttta agtattatga ctagtaagcc agaaagtagt acatacagta
     1201 aatcaatgtc acttcttctt ccaacatgct ccaatactga actatttaga gctttcatgg
     1261 aagtatttaa taagtttcct ttagaaggtg ctaaaatacg ctctcttcat cttagcgtca
     1321 gcggactttc aagcgaccct tatgagcaac ttagtctcat tgaggattat gtaacacaaa
     1381 acactaaaga gcaaagaatg tgggaaacac ttgatgaact tcacgcgcgt tacgggaaag
     1441 ataaaataat gcgcgcgaac gcactcacgc gtgcgagcac ttatttacga cgcactaaac
     1501 aaattggagg tcacaataaa tgagtcgcgg gatgaaaaag tttttaccgt ttgcaagtct
     1561 aaaagaacag gcacttttcc tagcggaaat gcagcagcaa aaaaagcgtg ttgaaaagcc
     1621 cacactcagt gaagaccaat gcgcagaaat taatcgtgtt ttaagcgaat acaccggcga
     1681 aaccgtatta atttattatt ataactgcgg ttttattaag acgcatcaag ccaaaataaa
     1741 aaagattgat actttatata aacgcttagt gtttactgac ctaaccgttt catttaccaa
     1801 tcttttacat attgaaatta tttaattacg cttgtaattt aaaactcata agcacagggt
     1861 tatgatcact ataaacaaac cctaacccgt gacgcgggtc gctttcgggt aagttttctt
     1921 tttggagacc agggccacta ccaatcgttt taatttcagc taccgcgata ttatcactaa
     1981 caaggaaacc atcaattgtg accattgcat tatcaacaat cccgttatta tttttatcaa
     2041 taaatttaat tgaggcgtcg cgacatgtgg caatatttac cgggccgtga ctagatagcg
     2101 ccatatcata aaagtcatgc gtataatctt cttctaaacc ggtaataatg tttgggactc
     2161 ttgttccgtg ctttcctgag tcaaggcgaa aatagttata ccaattcgct tcaagaattt
     2221 ctgtttcttg tttattaaag atatttgctt tatattcttc gttaacaagc gggttatcaa
     2281 gcactaagtc atgattaaag tcgccaccaa cgacaacata gttattatct ccatcataat
     2341 cacgcgctac tctaattgcc gctttaagtt gttcaagttg tactttacga atgacaccac
     2401 ctttatcata agcgctcata tgcacattaa atacataaag ctttttaact gaaccttcaa
     2461 ttggtatttc actaatagtg aaagcacgat ctaaatcaaa gaatttacca aagaaggcat
     2521 ccgtaatttc aaattcacgg cgttcagctt tagcgatatg atatttagag tatgtcgaaa
     2581 tacctgaatt tgatttacca attggttcat gcagtgggta cattaaataa gcagagtgat
     2641 agttaatacc gtaaattgtt gcgtaatcaa cgtgtttttt attaccaatt tcatattgat
     2701 taactttata agactttgtt gaacttgtgt caacttcttg ataaagcata aagtctaaat
     2761 cacctaaagc tgcaattgtg tcataagcac cgttaatatt ccttagcact tgtgctttac
     2821 ttgtaccacg cgcacgatca ccagtgttac ttttattacc ttgcgaatga atatattcat
     2881 ctttaaaaac actcgtgtcc ataaagaaac tgtaatctct gtcatatgcc ccaaaaccaa
     2941 cattatatgt tgtaatctta aattcttttg taccaatatc agcgacactt aatttcgctg
     3001 tttgattgtt taatacttga agactagtgt caccaatgcg gtagtaagag aggctaacat
     3061 aacctatata accacctaag acaagcacca cgacacctaa aagcacaaac actgccttta
     3121 agataaagca taacggtcta cattttcttt tattttttgc cattaaattg cccaggttcc
     3181 ttcgttaaag attactactt cttgaccgta ttttgttgta ccgacaatcc gtaaatcatc
     3241 aacgccaatc ataaaatcaa catgaatcat tgagttatta attcccattt cacgaagttc
     3301 ttcaagagag tacttttcaa aatcacgaat acaattacta aacccaagac ctagcgctaa
     3361 gtggcacgaa gcattttcat cataaagcgt tgaataaaat aagagcccta attgattaat
     3421 tggtgattct ttcgggacga gcgctacttc accaagataa cgagcgcctt catccatatc
     3481 taacatttgt tttaataatt cttcattttg tttagcgcca aagtcgacaa ctttaccgtc
     3541 tttaaactca aaccaaaagc catcaattaa ttcaccttga taactaagcg gcatcgtcgc
     3601 tacgaccttg ccttcagctt ctccgcgctt tggtgttgta aaacattctt cagtcggaat
     3661 attaggatta tagaaaatat tactacctaa agttgtttca ccacccgcta aaaagagtgc
     3721 attaggaata aggccaactt taaaattagt gccgtttttg ctagtgtaat gtagtgaaat
     3781 gagtcctaat tcgttaagct tgcgagactt ttcgctcata cttcgattat gttcattcca
     3841 attagcaatc gggtcgccat aagcacggct cgtcgttaat attgcgtccc ataatttatt
     3901 gcgggcttgt tttgcggtta agtctggaaa taatttcttc gcccactttg taccagcagc
     3961 accggcaatt gtccattgat acttattttc catctcatca cgataaggtt taataacggg
     4021 gtaaatattt tgcattactt gcgcacgctt ttcttgatta atcccttttt caccatcagg
     4081 gtcttcgctt gtaatataaa ttctaacggg attcttatca acataatatt gatagcgttc
     4141 taactcccac ttttcaaaat gtgacattga tttaacagtg cggtatttat tatggagttt
     4201 actaatgtcg tcatcaatcc agtcaagcgt cacgcggttt gctttagcgc gataagcctc
     4261 ttcaataagc attaacacaa attcgcgttg ttcaagttcc gcagttataa taacatcttg
     4321 tcctttttgt acattcgctc cgactctaac aataagccgc gcatattttt taagatcgct
     4381 ttttttcata catttatttt ccttctttaa ttacaaactc tttaaagtgt ggcaaagtta
     4441 aacaccaatc acaaaaagta tgccactctt taagtttgtg attacgacgt tggaagtaca
     4501 tcgttttaag ttgttgatag ttagttgtca ttgtcgccgc aagacaaaaa ccgcttggta
     4561 aagtcgcaac aatcttacgc cacgccttaa cttttgcatc tttatcttct tcacttatgg
     4621 cgttataatc atttactagt tcttctaaaa tagggaaaac acgcttatca gtttcgggga
     4681 cacagcgttc atcaatctta aattttaaca gcatatgcat tgttgattga ctactaataa
     4741 agtcaaacca gtggtagcgt tgtgcttctt tccaccagta aaaaggagcg gtaatatcaa
     4801 catgcactaa gatccctttt aaaaaattat catgaccttc gccaccttta gtcgttccga
     4861 gtagcactgc tcgcttgata tctttttcat gtagcgcgcc accttcaatt tcggtccgca
     4921 tcggatttcc gctcgcttta acggcgcgct ctaaaccgta gacaacactg ttttcaattt
     4981 taaattccat gtaaataagc ctccattaca ttttctaata aagttaatct agttaataac
     5041 ccaacgccac ctggcactgg agtttgtaaa taaacatcac gccctggttt aatgtcacca
     5101 gttaattttc cctcttcatc acgattaatc ccaacatcta caagaactgc actattttta
     5161 agtgtatgtt cgtcattaat aaagtgttta cgccctacgg cgacaaccat aatatccgca
     5221 ttagcgacat aatttttaag gtcttctcta cttgtctttg aatgtaatac tgttacatta
     5281 ccgtcttctt ctaaaaagag catagcagca ggtttaccaa caatattgct gcgcccaata
     5341 atcaccacat tcttcccgcg gatttcaaca ccttcatctt ttaaataatt aatgatacct
     5401 agtggcgtac acgctttaaa gcgagaaagc ggatgaaagc cgtccacatc tttagcggga
     5461 ttaatagctt ctttaacgat attttcatca atatgttttg gcagcggtaa ttgcacaata
     5521 atgccgttaa catgtggatc tttattaagt ttttttattt tcttaagcaa ttcatcaagt
     5581 cttgttgttt cgggcatttt aataagcttt gcgctgatgc ctacttcacg ggcgtcatta
     5641 atcttacctg caacatattt attgctcgca aaatcatcat ttacttgaat gataactaaa
     5701 cttggggtgc ccccatcttt tagtattcgt tcttttaaat tttgtttacg ttttaaaaca
     5761 taagttttaa tatccataaa tgcacctcat aaaataaatt ataacacgag agcaaaagcc
     5821 tagatatcta agacaataaa aaagatgagt ttgcacgatt aagctaattc taaagactct
     5881 aaaatttaat agtcgcttag taaattagtt ttaaattgtt tgttacatat ttttcaatga
     5941 tgattaccat ttaataaatc atcaatctga tcttcaactg ttgtaaatcc gcaatagttt
     6001 gcaaatgcta agaaaaatat tgctttttgt aaatttccgc ctctattttg taattctgtt
     6061 atcaccgtgt tcgttccttt tacattatct ttcctatttg caagtaattt ttttattgtt
     6121 tttggtagtt gcacacaaaa tttataaaag gcattttctt cacccgtaac aattttgtaa
     6181 aattcgtcaa ttgaaactcg acgtatacgc tcatcatagc agggttcatt atctaattta
     6241 acactccata caatatttct actttgttgt gctataactt caactaaaaa acatttatca
     6301 tttgggtttt cttttatgtg gttatgcatc attcggtaag tttgttccgc tgattttgaa
     6361 ttcattgtat tatgtttatt tttagttcta catatatttt ttgtcccgct tcgttagtgt
     6421 aaataatatc aaacccagtt ttaggaactt cacaacggtc aatatattta aaaatatttt
     6481 gatgaaaatc accaagagca ttcgtaattg ttttatcacg ttgtctatta atttcagtgt
     6541 cgataatttc tttccaagtt ctgtctaata aatacttgtc aattgataat tttattggat
     6601 ctataaggtt tgaattgaaa cgttttaaat cataatcact aagtatttca tagtatttat
     6661 caattaattc atttacatgt ttttcaaaat tttcttgagt aataaaagtt agagtatagt
     6721 catcattcac cttttcgtcg atagtcatta ttcatttatc ctttcaagcg ttttaattaa
     6781 ttctaaacct atttctttcg ctaagttgac tggtacagca tttccaatta gtttatattt
     6841 tgaactaagc gtgccagaaa aaacccaatc gtcaggaaaa gtttgaaatc tagcattttc
     6901 acgataggaa aaaggtctaa cttctaaagg atgacatctg tcggtttgtt tcataaatgg
     6961 acttgtgagt acagtgagtg atggctcatc taaacttagt ctacgtaata tgcctgttct
     7021 tcctccaccc atatcccaac acgacttcat atactctttg gcagtttctg gttcaatatc
     7081 tcgccaatag ccaccaggag gaacaagttt aaatatctct tccttgtatt ttgaatagcg
     7141 tttacaatgt tctttatcag gatttacatc aagtttaaca tctttaatgg tcggcttata
     7201 tttatgcttt ttcgggaact cgaaaattcc ttcttcaaat aaatcttttc taatcccaat
     7261 cgtaattaat ctttctcttt tttgtggaac actataatcc caagcattta acacctcata
     7321 aagtgtaata tatccttgtt cttcaaaaat atcgagaata gttttatatg ttcttccttt
     7381 atcatgggat aataaaccac gtacattttc aaataaaaat aattttggtt gtaattttcg
     7441 cagaaatgtc gcgtaataat aaaacatcgt tccgcgtaca tcttctaacc caagtcgatt
     7501 accagcgtag ctaaatgatt gacatggtgc ccctccggaa attaaatcaa gttcaccttt
     7561 ttttatatta aattcttgct ctaaatcacg ttcacacacc tcgcgaatat cttcaagaag
     7621 aacattccat tctggtctat taatttttag agtatcaaca gcatttttct caaattccac
     7681 tagaccaata tgatcaaaac ctgcttgttc aagcccaagc gctaatccac ccgctcctgc
     7741 gaataattca attgacttat atttttcttg ttttgcaatt ggtttaattt taacttgatc
     7801 aaataattca tcaagtttta cttttgcgat aattgttggc tcataatgac cattttccca
     7861 ccggttaacc gatgaaaaag aaacatttaa aatatttgct aattgttgtt gtgaaacatt
     7921 aagtgttctt cttaatttat gaaggctttt tgggtaatca atttgtatca tataacttat
     7981 ctcgcttcca aaaataatat agcactttat ttcactttta taattttaat actttcctta
     8041 tttatttata aataagtttt tgctgtggtt tattacaact actaataaat acaataaatt
     8101 taataaaaac ttctcaatat tatacataat aaaaaaacac cctgccgggt gttaatttaa
     8161 tagttttaat aatcttaacc taaagcttta acaaggattg caaggagaaa gaacaaaata
     8221 gcaaggccgt aagtaatata agaaattact ttttcacttc ctctttcttt tgttttgacg
     8281 aaaatattaa ggccgccacc agtgatagcg ccagatgccc cttcactttt accaccttgc
     8341 ataagcgaga taataataag taagattgat acgataatga agataatatc taatgttccc
     8401 atagtcttcc tccagttctt tattaatata cactatttat aaattaattc aagaaaacat
     8461 tatttaaata tacatatatt tatgtttttt tactctttcc ctctaattca aagagaattt
     8521 cacgatattc tatcgctttt tcaaagtcat aggctttagc ggcagcattc atcttagcgc
     8581 gaatttcccg aatatatttt tctaattgtt tacggctcat taagccttta ttaatccgct
     8641 caagttcacg ctctgtcccg aaattatgga gtggttcgcc aagtggtttg ataattgtgg
     8701 tgggaacaat tccgtgcttt ttattataag caatttgtaa atctcttctt ctttttgttt
     8761 ccgcaatagc gcgttccatt gaccccgtca tattgttcgc atacattatt actttcccgt
     8821 taacatttcg actcgcgcgg cccattactt gaataagtga tcgctcacta cgtaaaaatc
     8881 cctctttatc cgcgtcaata atcgccacaa gtgaaacttc aggaatatct agtccttcac
     8941 gcagtaaatt aatcccgaca agcacatcat atttcccttt gcgtaattca taaattattt
     9001 gactgcgttc cagtgtcttt gtttcattat ggagatagac aactttaaag tcatgctctt
     9061 ttaagtaatg ggttaggtct tccgccattt taattgtaag cgtgacaacc attgctcttt
     9121 caccacgttc aataactttc gttaattcat acattaaatc atcaatttgt ccaacactcg
     9181 gtttaataaa tacttctgga tccactaaac ctgttggccg gattagttgt tcaacgacta
     9241 ctccacctgt tttatctaac tcataatcac caggtgttgc gcttgtgtaa attacttgat
     9301 taatcatctc ttcaaattca ttaaattgta gcgggcgatt atctaaagca ctcggtaatc
     9361 taaaaccgta gtccactaga ttttgtttac gactgcgatc accattaaac atccctctta
     9421 cctgaggtaa agtaacatga ctctcatcaa tcacaagtaa ataatcttta ggaaaataat
     9481 ccattaaggt ataaggcttt tctccagctt tacgcccatc aatataacgc gcataatttt
     9541 caatcccagg acaaatccca aattcttgga gtgaatcaac atcttgtctt gttctaaatt
     9601 ccagccgttc ggcttcaagg agtttacctt catcggcaaa gtaacttaat ctacttgcta
     9661 agtcacttaa tatttttggc acaatggcat ttatccgttc acgacttgaa gcataggcat
     9721 atgcgggaaa aattggataa gtgttaaatg ttttgcgcac ttcaccagtt aaaacatcta
     9781 tttctctaat cttttcaata gtgtcgtcaa atgtatcaac ttgaattaaa tagtcttcac
     9841 tgatagggag cattatttca attacgtcac cccgtactct aaaagtacca gcgcgaagtt
     9901 cgtaatcatt acgtgtgtat tgcgcatcca cgagtttaat taaaaactct ttgcgattaa
     9961 tcacttcacc ttccacaata ttaaaggata gtttactata ttcttcaggg tcacttaatc
    10021 cataaattgc cgcgacacta gcgacaataa ttgtgtcatt acgctcgagt aaactattaa
    10081 tagcagcaga gcgaagcatt tcaatttcgg cattttgcac tgcttctttt tcaatataca
    10141 tatcgcgtga tggcacataa gcttctggtt gataaaaatc aaagttagac acaaaatatt
    10201 ccactctatt attaggaaac aatgctttaa attcagcgta aagttgcccc gctagtgttt
    10261 tattatgcac taagatcagc gttggttttt gcactttttc aataacatta gcgatggtaa
    10321 aagttttacc agtgcctgtt gctcctaaaa gtacttgatg cttatcaccg cgctctagcc
    10381 cagctactaa ctctttaatc gccgttggtt gatcaccagt gggcgtaaaa gtcgtttcta
    10441 aaacaaattt atgctttgtc tttttcatct ttctttttaa tatttactaa gccttcttta
    10501 actggattta aactttcaaa attaccgcct aacactactg ttgacccttc aatactcaca
    10561 aaagcttttt tatcgatttt agcaataccg tttattaaac gagcgtaatt actacgatca
    10621 aaatggacaa caaccatcat gtactcatct ttttgataac cacctaaaac tggcactaag
    10681 gttgtacctt gaccaatttc gttttgaata aaatcattaa tttctgcact tttttcacga
    10741 cttataatcg aagcagtcat cgcatgcttt ttaccaacga agacaaattc aagagtaatc
    10801 gcaaacacaa atgcactaag aataccgatt aaagagagta caacatcttt tagcacgaat
    10861 aacccaacgg caataattaa gccatcaatc acaaaagaca taacactata acttagtctt
    10921 aaatatttat ttaaaattac tactaaaata tccactccgc ccgttgaacc gccacctcta
    10981 aaagttaatg ccacaccaac accattaaac acaccaccaa agacaccggc aataagttta
    11041 gtggcaacat cagcgccctc accaaacaat ttaacggttt cagttgcaat tgctggtaaa
    11101 cgcatgagta aaataagcgc taaaggataa acaaatgaac taacaagcga tttaattgta
    11161 aagcgccaac ctaagaataa taaactaata aagaagaaag cccaagtcat cacaagtacg
    11221 actaaatcag cgtcaaaacc aaataagcct tcaccaataa tgccgacacc acttaacccc
    11281 ccagtattaa gtgttgcggg taaaagaaaa acaccagtac caaacgctag taaaattgat
    11341 ccaacaacaa ttaaactata gtttttaagt aaagttctcc aatcctcttt aaatatatct
    11401 tttattcttt gtttagccat tgcttcttgc ctccatttca aggtatttat acataaattc
    11461 cgctatgtca ccgtccatca cccgtgcaat atcaccgact tcataatttg tgcggtgatc
    11521 tttaataagg gtatagggtg caaaagtata agatcttatt tggctacccc attcaatcat
    11581 cttcttttca ccttcaatcg cgcttacctt tgctttttgt cgttctaatt ctagttgata
    11641 tagttcactt ttaagcatcc gcatcgcaat ttcacgattc attaattgac tgcgttccat
    11701 ttgacacgat acaacgatac ccgtcgggac atgagtaata cgcactgcag tctcaacttt
    11761 gttaacattt tgaccgccag caccaccact gcgataagtg tcaaccctta aatcactatc
    11821 attaatttca atatcaccag tttcatcaaa aattggcaca acattaacac tcgcaaatga
    11881 agtatgtctt cttttattcg cgtcaaaagg tgagatacgc actaagcggt gtactccttt
    11941 ttcactacgc aaatgaccat aagcattatt cccttcaata ataagtgtta cactttttat
    12001 gccggcttcg ttccctgctt ggtaatcgac aactttaagc gaaaaacctt gtctttcaca
    12061 aaagcgtgca tacatatgat agagcatatc ggcccagtcg tgcgactccg tcccgcctgc
    12121 accagcgtgg atacttacgg ttgcatcatt ttcatcatat tccccgctga aaagcagaag
    12181 atttataagt aattcacctt ctttttgcgc attatcaatt tcaaatgcta aatcacttaa
    12241 atactcacta tcctcggcaa aagtgtcgtc ttttagtagt tcaaaggcac tttccactgt
    12301 tgtttcaact agtgaatata tattaatttt ctctttcgca gcatttaatt ttttaatgat
    12361 tgttttagcc ttattttgat tattccaaaa gtcaggatta tttatttcaa tcgttaattt
    12421 atcaattgtt tcttttaaac tagcgacatc aaagagagtc actaagctta cctagtaatc
    12481 ttttaagttc attagcttta cttaaggcaa ctttgtactc catcgtttat tttttatctt
    12541 ccttttttat attttccttt tcggctgttt ctttattttc ttcagtgcct tcttgacctt
    12601 cttcaacagg tggggcaaca ttaatcttcg cgtttaataa atttaaaact gtttcaacag
    12661 caataatatc catcgcttca ccgaatagct tataaccttc attaacatag tcttgaagtg
    12721 ggttagtatt tgcgtaacta cgtaagtgaa taccttcgcg taagtgcgcc attgtgtcaa
    12781 tatgccgtgt ccagttgcgg tcaattgccc gtaaagtaat ttcacgttca acgcggtcgg
    12841 caagttcttc actccattca cgtcttctaa gttgataacg acgatacatt aaaagcatta
    12901 agtcttcacc aatttcttct ggtggtgatt cgtcatacgc tgaagggtta atcgaacctt
    12961 ctggtaaata gtttggttcc ataatttgtt ttaacattac cccattaaca agcgtatctt
    13021 taccagtttc tggaacagct ttttttgcta aataaagacc cgcaagagta aactgttctt
    13081 gcactaagtc tttaatatca ccagcgtata ttatgctgtc acgtttctta taaacgatag
    13141 agcgttgttt tgctaaaaca ttgtcataat ctaataaact tttacgaata tcaaagtttt
    13201 gtccttcaat tcgtttttgg gcactagtaa tagcgttagt gaacatttta ttttctagtg
    13261 cttcgtttcc catcccttcg acgagtttaa gcatcatatc actagagaaa cgacgcatga
    13321 gtaagtcatc aaatgagaca tagaagcgtg aaaacccagg gtcaccttgc cgtccactcc
    13381 ggccgcgtaa ttggttatca atacggcgag cctcatgtcg ctctgtccct aataccgcaa
    13441 ggccgccaag tgctttaact tcatcattaa ttttaatgtc agtaccacga ccagccatgt
    13501 tcgttgcaat tgtaatttgg cctttattac ccgcaagcgc aacaatgtct gcttcacgcg
    13561 cgtggttttt agcgttaagt acctcgtgtt tgagtccgtt tgctgttaaa agttcactaa
    13621 cttcttcact tgattcaacg ctgcttgtcc caataagaat tggttgtcct ttagcgtgac
    13681 gttctttaac ttcttcgaca agtgcattga gtttatcttc tttacgtgca tatactcgat
    13741 cgacagcgtc ttcacgaata acagggcgat tcgtcgggat ttgaataaca cgcatattgt
    13801 aaattttgcg gaattcttct tcttcggttt tagcagtacc tgtcatcccg cttagtttag
    13861 tgtaaaggcg gaagaagttt tgataggtaa tcgtcgccat cgtcactgtt tcttctttaa
    13921 tcgtgacatt ttctttcgct tgaattgctt gatgcaaccc atcactatat tcacgaccgg
    13981 gcataatccg tccagtaaat tggtcaataa gttgaatttc acgatttgcg tcaaccatat
    14041 attcaacatc acgactcatg acataatttg ctttaagcgc attatgaatc cggtgcacta
    14101 agtcactata ttgtgggtca taaatattat taacaccaaa cattctttcc gcgagtttat
    14161 taccttcgtc cgttaagtta catgtttttg ttttaatatc aacgacaaag tgcttatctc
    14221 tgcgtaatgt tttaacgaaa cgatcggcga cattataact agatgcttgt actttagcgc
    14281 caccagaaat aatgagcggt gtgcgtgatt cgtcaattaa aatcgagtca gcttcgtcaa
    14341 caatagcgta ctccaaacct cgtaatacac gaccatcaac actgctagcc atattgtcac
    14401 gaagatagtc aaaaccaact tcactatttg tagtgtaagt aatatcacat aaatattctt
    14461 cacgtttttc acgcgctgtt ttttcacgta ggttaacacc cacagttaaa ccaagaaagc
    14521 gataaatttg acccatccaa tcggcgtcac gagcggcgag atattcgtta acagtaacaa
    14581 catgcacacc tttgcctgat aatgcattaa ggtaaacggc cattgtcgca gttaaagttt
    14641 taccttcacc agttctcatc tccgcaacat caccatgatg taagacgatt gcccccataa
    14701 tttggacggg ataagggaat tgtcctaaag ttctacgtgc tgcttcgcgt gccacagcga
    14761 aggcttctgg taataagtca tcaagcgttt ccccattagt gagtcgttct ctaaattcgg
    14821 gtgttttagc ctttagttca tcatcagtta aagcttccat cgttttttca taagcaataa
    14881 cttttttggc tgttttttct atttttttga ggcgcctttg ttcaaagcgg aatatttttt
    14941 ctataaaact catatacact tgtattatct aatcatgccc cggtacttcg attaggtaat
    15001 acatctcctt tca
//
LOCUS       DAOSLP010000024        14340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_4320_length_14340_cov_7.723836, whole genome shotgun sequence.
ACCESSION   DAOSLP010000024 DAOSLP010000000
VERSION     DAOSLP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 14340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14340
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4320_length_14340_cov_7.723836"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1088)
                     /locus_tag="PK460_03230"
     CDS             complement(72..1088)
                     /locus_tag="PK460_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54127.1"
                     /translation="MKKRNLLLALVGSALLLTGCKGGSSEKPVSETPAVQKSFGFGSL
                     AKYAATRADNEFDIETTVDYVAVVLEADVIKNLRLDTVQLYAKIAEEAVVLKTETNGD
                     VKSKWELLDGYGMKVASPIEKEWYEQAEAFENWAVGKTVAEVKAGMTGTKLTDGATIG
                     NTIHVNAWVAALEDAVLNKVTFEGEVKGLGVGGVNAVSKDGATGAVEGQDWTIGGAVF
                     TEGKKVLAARIDTFQVRYENSEGKAVIKSGLKQVDYDNKRVKGKQELKEEYGMKVASP
                     IEKEWYEQAASLVEYIKGKTVSEALGTAAKLANGAEVGCTMNIGGYRTALLEAEHTAF
                     NSRK"
     gene            complement(1161..2471)
                     /locus_tag="PK460_03235"
     CDS             complement(1161..2471)
                     /locus_tag="PK460_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOE54128.1"
                     /translation="MKLIKKLFALFSPLELVILMVSITISTLLVVPAFSKDTQKKTPI
                     LLFPTEKVYNTDGGRPIEVFDTSFFVVYEEVEEQTQNDIKAILNEYLISYHKLFDRHN
                     DYYAVEPHDNIHPTAEEKATLPRIKNLKYINEHKGEEIEIHQALYDLLVASKSYSINT
                     PNNAFSMFIGELYDYWKPYTSPGADPTKDPVRDDLKQAEIARLTSYIPQSEDDINNTL
                     KLWTNDNKYYVKFNEFNHSGDDLSISVGAVAKGMMTDILAEAMRAKGLTKGLIYGGQS
                     SFTFLEDGFMNKAYKITMEPIGPGEKTFTMSRADQYQMSTSGIYTGFKFNYGGAQIIR
                     SHIINPLTGYPAHNSHHMVNITSDTLSGLTLDYLTTALTVLSVQDGLTFLSEEYPSDD
                     INVIYSGYFNDEWFVAFTNGYPGGKTPSFQLAKEYREINLEFLL"
     gene            complement(2458..3012)
                     /locus_tag="PK460_03240"
     CDS             complement(2458..3012)
                     /locus_tag="PK460_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOE54129.1"
                     /translation="MSQVSNNGNKRKLSTQKLVRLAVFLALGVVFNIVENYITFIPAL
                     PGVKLGLANSIGLILLALYGPLEFFTIGLLRVLLSGTFSGFGTSFILSLSGFLLSSAF
                     VLLAYLFNKLSIYGLSLLSAVMHGVGQVVMIAIIYQNALMLNYLIIMTISGIISGLLI
                     ALLSKIVILRLKMFKEEECNYEIN"
     gene            complement(2993..3406)
                     /locus_tag="PK460_03245"
     CDS             complement(2993..3406)
                     /locus_tag="PK460_03245"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOE54130.1"
                     /translation="MKARKVKLNKTLFDTIIFSIVLVLTSATTLYLKRAFSTTEALYV
                     YVYVTDRLIDKQPLAIDNTFIYNEPEFLAPITLEIYNKKVRVEKEESPLNLCSIKGFT
                     DKVGDPVLCLPNSFYFVIMGFNYKEAPSPYVPSVE"
     gene            complement(3430..3744)
                     /locus_tag="PK460_03250"
     CDS             complement(3430..3744)
                     /locus_tag="PK460_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOE54131.1"
                     /translation="MQYFWGVVILLVIGALLGLLLAVASKAFAVKEDPRLEPITEMMP
                     GINCGTCGYPGCKELVVAMLKGEVKNLGQCRPLRPDAKAKIKEYLANHPDEEGNILTV
                     QG"
     gene            complement(3748..3930)
                     /locus_tag="PK460_03255"
     CDS             complement(3748..3930)
                     /locus_tag="PK460_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54132.1"
                     /translation="MSDVARIFVAIGVMAILIALYIGSYVLNKRTKKPEGCKDFASSC
                     SGCAVTSCSHNPLKEE"
     gene            complement(3932..4522)
                     /locus_tag="PK460_03260"
     CDS             complement(3932..4522)
                     /locus_tag="PK460_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOE54133.1"
                     /translation="MQFLLMGIGAVLINNIILTRFLGICPLLGVSKSNSSAIGMSLAV
                     IFVVTMSAILSYSLYMYVLVPLKIEYLRLVSFILIIASFVQFVEMFLKRFVPALYKSL
                     GIYLPLITTNCVVLAVSLDVVATNGGVPVYNFLNMVGYAFFTGVGYAVILILFSFIQE
                     RLINNKNMPKSFQGSGIALVTIGLLVMAFLGFAGMI"
     gene            complement(4525..5214)
                     /gene="rsxE"
                     /locus_tag="PK460_03265"
     CDS             complement(4525..5214)
                     /gene="rsxE"
                     /locus_tag="PK460_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HOE54134.1"
                     /translation="MKKRKIFIDGIFANNAAFMLFLGMCPALATTSSFMSALGMGIAV
                     LATLILTNVVISAIRRWVPNEIRIPVLIVIIATTVTIIEMLMQAYMFDLSQTLGVYLS
                     LIVVNCIILGRAEAFAMKNNVLDSLIDAVGTGIGFMLGLLALAFAREFLGTGGIAFTS
                     LLTGQPIWGFQIIPDNYTIGLFQESSGAFIMFGLIVALINFVVAKISENKKKKEAAAK
                     AAAKLAAEGGK"
     gene            complement(5217..5774)
                     /locus_tag="PK460_03270"
     CDS             complement(5217..5774)
                     /locus_tag="PK460_03270"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOE54135.1"
                     /translation="MSKKKLNNFILLPLFLGAVTLVAAGALTGVHLLTKPKIEENIKQ
                     KQLSGYKAVLDLAQSDVIDLKKETVSDELAGIGVTNKFSVTIDTTLVGVVYDAEITGY
                     NSGLIFQVGFKGTKYAGFNVISSNETPGYGADYLETVHEQIKGVEIGDPILQDPSKTG
                     KTITANALRKALEACATDYLEGRAG"
     gene            complement(5776..7614)
                     /locus_tag="PK460_03275"
     CDS             complement(5776..7614)
                     /locus_tag="PK460_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOE54136.1"
                     /translation="MKIVSGNAPFLRSKRTTSQIMMELLIGLAIIFVAAVIYNFTLGV
                     RYGLKTICIMTVAILFTFISDIIAGSLRYNKEKDGKFDEYLIHFIKSNYSVVTAVIFA
                     LTLPIGTPVYVVIIGSMFATLVVKHAFGGFGSNVFNPAALSRIFLALTFGSQLKAYLP
                     GGENIGGLVAGATVTSQYAADGVKFLTGSLANTNVSMLDLYLGNHSGAIGETFTLLIL
                     IVGIVLAAREVINWRTPVFFLGTVALSSVFVSLVAKVNMIEYLLVQFGVGGMVFGAVF
                     MFTDPVTSPTSNYGKALIGIIGALINVLIRIAGVYPEGTAFSIAFVNIISPMIDRLIT
                     GRTNNKVWVPATVSGVMVLGSVGLITGVSAAKMPRVAPEPPPIVVEPFVTYLGSYTSP
                     TPSEYAEEFTTNVEVGLSDAYHIVKIEYDVIANPDNYKKVTDGIDAVIDYYTSISVAE
                     FKALPVPALSEGTTGSTVAGQTGEHIIPGAIYSSRALYEAINDALKDLNVYSADVEDY
                     PYDSGGYSDETLVMSVDVYVDTVTDLIKVVDIKDGASTGTYADRWETVKEAVLSKYHD
                     LDVATFLGYTNVAELYGEEVATYAGVTYSARRLFNAVQKALEGYGG"
     gene            complement(7626..8927)
                     /locus_tag="PK460_03280"
     CDS             complement(7626..8927)
                     /locus_tag="PK460_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOE54137.1"
                     /translation="MKLFQTVGRRKIPGYKSMSLERELVVLDPKKVEYVYFPLVAANN
                     VPLEVFVKEGDKVKVGTKIALKQQFSVPLFSSVSGTVRAIEKRLNVTTGRPCDHLVIK
                     NDFKMTKEKPLKKVELDASKEDIVAAIKEAGIVGLGGANFPTWVKYNNVKDIHTILIN
                     AVECEPYLTTDYVSCSLNAAKVISGARYLQKAAGASKVIVAIKVKKEQACNCLKEAAA
                     PYPDIEVREVPDRYPMGWERTLIQTIFKKQYDKLPSEIGVIVNNSTTAIAVHDALVEG
                     SPIVSRIVTVSGNAVKNAANIVVPVGILAETVLNEFEVEEKEVVLLVGGPMTSNPAKD
                     VSFALTVCHGALTLLEKVVYRPVACLRCGSCTAHCPAGLQPIEILRAFEGRDYERLEK
                     LETLRCVDCGLCSYVCPSKIEVTDMMKKAKTLLRVHQSRKK"
     gene            complement(9037..10380)
                     /locus_tag="PK460_03285"
     CDS             complement(9037..10380)
                     /locus_tag="PK460_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOE54138.1"
                     /translation="MLKQIKTQREAMKKNFPLSYEKRRELLINLQKAVKLYEKHIYEA
                     LYKDLHKDEWEVFVTELGIVYEEMHFAIKNLKKWMRRKKIKSFLVQFPAKNFLVPEPY
                     GQVLIMSPWNYPLQLTLLPLIGAIAAGNYAVVKPSAYAPEIAKVMQQIVATAFPNKEA
                     ILFLGGRKVNEEILQGVYDFVFFTGSPGVGQTVMRKASENLTPVVLELGGKSPLIIAE
                     DADIPLTIRRLKFAKLINCGQTCVAPDYVLLDKKLIDKVDDIRQAFSVEGEEKARLPK
                     IINEKHYLRLKGVLTEVGSDNFDEETQTINPTVVLNPPLDSELMREEIFGPILPIITY
                     ETLDEAIAFINERPKPLALYLFTKSKEIMNKVLRETTSGSVAINDAVVQLANPHFPFG
                     GVGNSGMGSYHGYHTFKTFSHMKAVLHKSRAFDFRFRYKPGPKTVGIFKKLTRNK"
     gene            complement(10364..11578)
                     /locus_tag="PK460_03290"
     CDS             complement(10364..11578)
                     /locus_tag="PK460_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE54139.1"
                     /translation="MSKLGDRIFQKVLRTFSPILPWREPEILKAYDELVDVLIKQNKS
                     RIFIVMDEGLVQLKLHQPLLDALKNKQLNYRVFSDITANPTTAQVEEGLLAYIIYGGD
                     AIIGFGGGSPMDVAKAIAARVARPRKNLKQLGGLLKVRKKTPLLIAVPTTSGTGSEAT
                     VASIVVDERTKHKYAINDPVLIPSYALLVPTLTFKLPKFVTATTGMDALTHAIEAYTN
                     KGGRKFPNEKAINATRLVFENLKVAYDNPSDLEARKNMQLAAYDAGVAFTRNYVGYVH
                     ALSHPLSAYYGLAHGYVNALILPFMLEKYGRRIYRKLARLARLSGVVEEGLSNGETAK
                     LFIAKIRALNTYFEIPTTIPEIKEEDIPQLAVYANKEARPLYPTPRIYKTSELEEFYR
                     ELKGEIFNVETN"
     gene            complement(11742..12197)
                     /locus_tag="PK460_03295"
     CDS             complement(11742..12197)
                     /locus_tag="PK460_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54140.1"
                     /translation="MSKKRRIKELEAQVAALEAKVEGKEGQQTPKKAAKNSVNVYFDS
                     NFVNSLVSNQMKYGRRSRRDDLNVDPADKKAVRRARARRFFRFLWRLIVVLVIAALVA
                     AVVAAILMVIVWALVNFNIMTPTSNPFVGMVWEWIKWFFALFGMEVPIF"
     gene            complement(12216..14015)
                     /locus_tag="PK460_03300"
     CDS             complement(12216..14015)
                     /locus_tag="PK460_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54141.1"
                     /translation="MKIKTRLDFIVIDGTELVVNEALKTLAKLPVSDLVEFLKGPKLY
                     SRRDHANILKNVMRDEVWKFKLVSRVAEKGLSNDFFSRLDYFEDSPVTVLEGLFERMV
                     KIQPELMDYYLEDLWLFAIQVVSKDKELAKLYKKAQKSSTSPVVLKELFPTLEGLFGD
                     EEGDIDGVLARTFVEKSLHSATIKEIREIAEKNDVLLPTNITKQDIVDGIIAVLPKVA
                     KAKDVPHLTEDVKDMTVAQLKEFVNKHKLDVPTELKKQDVGELLVKNYVNKNAANVVS
                     DLQYELPERVEEATDFSDENPKVIRLRERVKELEKELEERPVENETKVDESELLKEQE
                     KVEALSKELKQTKAELSEKPKEVVVEVPGPTVIRTVISKKELQKQKEHYEAIIAEQEK
                     ERSMLISRTEYDEVLAEYGRVKALLDEKDIEKLEEKPVEPKPAPQQVPQYEEPYYGSL
                     HAKVDRLTDKVHELQLEILRKELANQKATPAPQEEQPETEVNIYFDSNLAKRMSDNKN
                     AIDVGKAVKGKKRKKKSVGRTIFWIIFILLLLAIVVFMGAWTLEHFNITPLAGPPWLA
                     DRFNAAVLWLHNAVQSVFDLFKGLFGKVRVLGR"
BASE COUNT         4362 a         3003 c         2378 g         4597 t
ORIGIN      
        1 tatcgcatat taacaaaaaa ggaactactt tgaagtagtc ccttgtgtat cttttcttcg
       61 taactaatta attatttacg actattaaag gctgtgtgtt cagcttcaag taaagcagtg
      121 cgatagccac caatattcat agtacaacca acttcagcac catttgctaa tttagcagca
      181 gttcctaaag cttcgctaac ggttttacct ttaatgtatt cgactaagct tgcagcttgt
      241 tcgtaccatt ctttttcgat tgggcttgca actttcatgc catattcttc tttaagttct
      301 tgtttacctt ttacgcgttt attatcataa tcaacttgtt ttaagccaga tttaataact
      361 gctttacctt cactattttc atagcgaact tggaaagtat caatgcgtgc cgctaagact
      421 tttttgcctt cggtaaagac agcaccacca atggtccagt cttgtccttc aacagcacca
      481 gttgcgccgt ctttgctaac agcgttaaca ccaccaacac ctaatccttt aacttcacct
      541 tcaaaagtaa ctttattaag gacagcgtct tcaagggcgg caacccaagc gttaacatgg
      601 attgtattac caattgttgc accatcagtt aatttcgtac cagtcatgcc agctttaact
      661 tcggcaactg ttttaccaac ggcccaattt tcaaatgctt cagcttgttc gtaccattct
      721 ttttcgattg ggctcgcaac tttcatgccg taaccatcta aaagttccca tttagattta
      781 acatcaccat tagtttcggt tttaagtaca actgcttctt cagcaatttt tgcataaagt
      841 tggacagtgt caagccgtaa gtttttaata acatctgctt ctaagacaac agcgacataa
      901 tcaacagttg tttcaatatc aaattcatta tcagcgcgtg ttgcggcata ttttgcaaga
      961 ctgccaaaac caaatgattt ttgcaccgct ggtgtttctg aaactggctt ttcactactt
     1021 ccgcctttac agccagttaa tagcaatgca ctaccgacta atgctagtaa taagtttctt
     1081 tttttcatat atatcattct cctctatttt cttcttactg cgcatataat tttaataaat
     1141 attattaaga aaagcaacct ttatagtaag aattcgagat ttatttcgcg gtattctttc
     1201 gctaactgaa atgatggtgt tttcccgcct ggataaccgt ttgtaaaggc cacaaaccat
     1261 tcgtcattaa agtaaccact ataaatcaca tttatgtcat cactaggata ctcttcactt
     1321 aaaaatgtta atccatcttg aacactaagt acggttaatg ctgttgttag gtaatcaagc
     1381 gttaacccgc ttagcgtgtc acttgtaatg ttaaccatat gatgagagtt atgcgcagga
     1441 taaccagtta gcggattaat aatatgcgaa cgaataattt gcgcaccacc ataattaaat
     1501 ttaaagcccg tatatatccc acttgtcgac atttggtatt gatcagcgcg gctcattgta
     1561 aatgtttttt cgcctggtcc aataggttcc atcgtaatct tataagcttt attcataaat
     1621 ccgtcttcta aaaatgtaaa tgacgattgt ccgccgtaaa tcaaaccttt agttagtcct
     1681 ttagcacgca tagcttcggc tagaatatca gtcatcatcc ctttagctac cgcaccaaca
     1741 gaaatcgaaa gatcgtcacc gctatgatta aattcattaa atttaacata gtatttatta
     1801 tcgttagtcc ataactttaa agtgttatta atatcgtctt cactttgcgg gatataactc
     1861 gttaaccgtg caatttccgc ttgttttaaa tcgtcacgca ctggatcttt agtcgggtca
     1921 gcccccggac tagtgtaagg tttccagtaa tcgtaaagtt caccaataaa catactaaaa
     1981 gcattatttg gtgtgttaat agaataactt tttgacgcaa caagtaagtc ataaagagct
     2041 tgatgaattt caatttcttc gcctttatgt tcattaatat actttaagtt tttaatgcgt
     2101 ggcagtgtcg ctttttcttc cgctgtcgga tgaatattgt cgtggggttc aactgcataa
     2161 taatcattat gacggtcaaa aagtttatgg taactaatta agtattcatt tagaatagct
     2221 ttaatgtcat tttgtgtttg ttcttccact tcttcataca cgacgaaaaa actagtgtcg
     2281 aatacttcaa tgggacgccc accatcagtg ttataaactt tctctgtcgg aaataataaa
     2341 atcggtgtct ttttttgcgt atctttgcta aacgctggaa caactaaaag ggttgagatt
     2401 gtaattgaaa ccattaaaat aactagttca agcggggaaa aaagcgcaaa gagtttttta
     2461 attaatttca taattacact cttcctcctt aaacatttta agtctcaaaa tcacaatttt
     2521 tgataagaga gcaataagta atccgcttat aatcccactt attgtcataa ttattaaata
     2581 attaagcatt aaagcgtttt gataaattat cgcaatcatg acgacctggc caacaccgtg
     2641 catcaccgca ctaaggagtg agagtccgta gattgataat ttattaaaaa gataggctaa
     2701 tagtacaaaa gcgcttgaaa gtaaaaaacc actaagcgat aaaataaaac tagtgccaaa
     2761 cccactaaaa gtcccgctta ataaaactcg aagtaagcca atcgtgaaaa attcaagcgg
     2821 accataaagt gctaaaagaa ttaagccaat cgaattagca aggcctaatt taacacccgg
     2881 taaagccggg ataaatgtaa tgtaattttc aacaatatta aataccacac ctagcgctaa
     2941 aaagacagca agccgcacga gcttctgtgt cgataatttt cttttatttc cgttattcga
     3001 cacttgggac atacggtgaa ggtgcctcct tataattaaa tcccataatc acaaaataaa
     3061 aagaattagg tagacaaagt acgggatcac ctactttatc cgtaaaacct ttaattgagc
     3121 ataaattaag cggactttct tctttttcaa cccgcacttt cttattgtaa atttcaagtg
     3181 taattggtgc taaaaattca ggttcattgt aaatgaatgt attatcaatc gctaacggtt
     3241 gtttatcaat tagacggtcg gtaacataga cataaacata cagtgcttca gtagtagaaa
     3301 aggcgcgttt taagtacaac gttgtcgcac ttgttagcac caagacaata ctaaagataa
     3361 tggtgtcaaa aagtgttttg tttaacttaa ctttacgcgc cttcatgtaa ataagagtaa
     3421 cctaactttt tatccttgaa cggtaaggat gttcccttct tcatccggat gatttgctaa
     3481 atattcttta atctttgctt ttgcatcagg acgcaacggc cgacattgac ctaaattttt
     3541 cacttcaccc ttaagcatcg ccactactag ttctttacat cccggataac cacaagtccc
     3601 gcaattaata ccaggcatca tctccgtaat tggctcaagg cgtgggtctt ccttgaccgc
     3661 aaaagcttta ctcgcgacgg caagtaaaag tcctaataag gctccaataa ctaaaaggat
     3721 aactactccc caaaaatatt gcataaatta ttcctccttt agtggattgt gactacatga
     3781 tgtcaccgca catccgctac aactactagc gaaatcttta caaccctctg gtttttttgt
     3841 gcgtttattt aacacataac ttccaatata aagggcaatt aagatcgcca tcacaccgat
     3901 tgccacaaaa atacgcgcaa catcactcat cttaaatcat ccctgcaaat ccaaggaagg
     3961 ccatcactag taaaccaatt gttactaaag caatcccgct accttggaaa ctcttcggca
     4021 tatttttatt attaattaag cgttcttgaa taaaactaaa gagaattaaa atgacagcgt
     4081 agccaacacc agtaaagaaa gcatagccaa ccatatttaa gaaattataa actgggactc
     4141 cgccatttgt ggctacaaca tcaagactaa ccgctaacac gacacagttt gtcgtaataa
     4201 gtggcaaata aatgccaaga ctcttatata aagctggcac aaaacgtttt aagaacattt
     4261 ctacaaattg tacgaatgaa gcaatgatta aaataaagga cacgagtcgt aagtactcaa
     4321 tcttaagtgg cactaacaca tacatataaa gtgagtaact aagaattgcg ctcatagtaa
     4381 caacaaagat aacagctaaa ctcatgccaa tagcactact attacttttc gaaacaccaa
     4441 gtagcggaca aatacctaag aagcgcgtta aaataatatt attaattaaa acagcgccaa
     4501 tacccattaa aagaaattgc ataattattt acccccttct gcggcaagtt tagcggcagc
     4561 tttcgctgcg gcttccttct tcttcttatt ttcactaatc tttgccacca caaaattaat
     4621 taaggcaaca atgagcccaa acataataaa ggcaccagaa gattcttgga ataaaccaat
     4681 tgtgtagtta tcaggaataa tttggaaacc ccaaattggt tgccctgtta aaagtgaagt
     4741 aaaggcaatc ccgcctgttc ctaaaaattc acgggcaaac gctaacgcta gcaatcctaa
     4801 cataaagcca atgcccgttc caaccgcgtc aataagacta tctaatacgt tatttttcat
     4861 tgcaaaggct tcggctctac ctaagataat acagttaaca acaatcagtg atagatacac
     4921 accaagtgtt tgtgaaagat caaacatata agcttgcatc agcatttcaa taatcgttac
     4981 ggttgtagca atgataacaa ttaatacggg aattctaatt tcattaggga cccagcgtct
     5041 aatcgcagaa atcactacat ttgttaagat aagtgttgct aatacggcaa tccccatccc
     5101 gagcgcgctc ataaatgaac ttgtagtcgc taaggcagga cacataccta gaaatagcat
     5161 aaatgcagca ttattagcga aaataccatc gataaagatt ttacgttttt tcataattaa
     5221 cccgctctcc cttctaagta atctgtagca caggcttcaa gtgctttacg cagtgcattc
     5281 gcggttattg ttttacctgt cttacttgga tcttgtaaaa ttggatcacc aatttccaca
     5341 cctttaattt gctcatgaac tgtctctaaa taatcagcgc catatccagg tgtttcattt
     5401 gatgaaataa cattgaaccc agcgtatttt gtacctttaa agccgacttg gaaaattaag
     5461 cctgaattat aaccagtaat ttcagcatca taaacaacac caactagcgt ggtatcaatt
     5521 gtcacactaa atttattagt gacaccaata cccgctaatt cgtcactgac tgtttctttt
     5581 tttaaatcaa taacatcact ttgcgctaag tcaagcacag ccttataacc cgaaagttgt
     5641 ttttgcttaa tattttcttc aattttcggt ttagttaata aatgcactcc ggttaatgct
     5701 ccggctgcta ctaaagttac tgctcctaaa aagagcggca ataaaataaa gttatttaat
     5761 tttttcttac tcatattagc caccatatcc ttctagtgct ttttgcacgg cattaaagag
     5821 ccgtcttgcc gaataagtta caccagcgta agttgctact tcttcaccat aaagttcagc
     5881 aacatttgtg taacctaaaa atgtggcaac atctaaatca tgatacttgc ttagtactgc
     5941 ttcttttacc gtttcccaac gatcagcgta agtaccagta ctagcgccat ctttaatatc
     6001 cacaactttt attaaatcag tcacagtgtc aacataaaca tcaacactca tcactaatgt
     6061 ttcatcacta taaccaccgc tatcataagg gtagtcttca acatcagcac tatacacatt
     6121 taagtcttta agtgcatcat taattgcttc gtaaagtgcg cgagaagaat aaatcgctcc
     6181 tgggataata tgctcgcctg tttgaccagc aacagtggag ccggttgtac cttcgcttaa
     6241 agctgggact ggtaaggctt taaattcggc gacactaatt gaggtatagt aatcaatgac
     6301 cgcatcaatc ccatcagtta cttttttgta attatccgga ttcgcaatca catcatattc
     6361 aatttttaca atatgataag catcacttaa tccaacttcg acatttgtcg taaattcctc
     6421 agcgtattca cttggtgtcg gacttgtata acttcctaaa taagtcacaa acggttcaac
     6481 aacgatcggc ggtggttctg gcgcgacgcg tggcatcttt gccgcactaa cgccggtaat
     6541 taagccaaca ctacctaata ccatcacccc actaacagta gcaggaaccc atactttatt
     6601 atttgtccgg cccgtaatta aacggtcaat cattggtgaa atgatgttaa caaatgcaat
     6661 actaaaagct gtaccttctg gataaacacc agcaatccga attaagacat taattaatgc
     6721 cccaataatt ccaatcagcg ctttaccgta gttgctcgtt ggtgaagtaa ctggatcagt
     6781 aaacatgaag acagcaccaa ataccatccc gccaacacca aattgcacta ataaatattc
     6841 aatcatattc actttcgcta cgaggctaac aaagacactg ctaagtgcaa ctgtgcctaa
     6901 gaagaagact ggagtgcgcc agttaattac ttcacgggcg gctaagacaa taccgacaat
     6961 taaaataagt aaggtaaatg tttcaccaat cgcgccgcta tgattaccta aatataagtc
     7021 aagcattgac acattagtgt ttgctaaact accagttaag aatttaacgc catcagcagc
     7081 gtattgacta gtcacagtcg cacccgctac taagccacca atattttcac caccaggtaa
     7141 ataagcttta agttggctac caaaagtcaa tgccaaaaag atgcgcgaca aagcagcagg
     7201 gttaaagaca ttactaccaa acccaccaaa ggcatgtttt acgactaaag tggcaaacat
     7261 actcccaata atcacgacat atactggggt gccaattggt agagttaaag caaagataac
     7321 tgctgtaaca acactataat ttgatttgat gaaatgaatt aaatattcat caaacttccc
     7381 gtctttttct ttgttataac gtaaactacc agcaataata tcactaataa aagtgaaaag
     7441 gattgcaaca gtcataatac aaattgtttt aagaccgtaa cgtactccaa gcgtaaagtt
     7501 ataaatgacg gcggcgacaa aaataatcgc taacccgatt aagagttcca tcattatttg
     7561 gctagtagtg cgtttactgc gtaagaacgg ggcatttcca ctaacaattt tcatattaga
     7621 cacctctatt tcttcctcga ttgatgaacg cgaagtaagg tcttagcttt tttcatcata
     7681 tcggtaactt cgattttact cggacacaca taactacaaa gtccgcaatc aacacagcgt
     7741 aatgtttcaa gtttttcaag tcgctcataa tcacgacctt caaaggcgcg caagatttcg
     7801 attggttgga gtccggctgg acagtgtgct gtacaactgc cacagcgcaa acatgctact
     7861 gggcgataaa cgactttttc tagtagtgtc aaggcaccat gacacacagt aagggcaaaa
     7921 cttacatctt ttgcaggatt acttgtcatt ggaccaccga ctaatagcac cacttctttt
     7981 tcttcaacct caaattcatt aagcactgtt tcggctaaaa taccgacagg cacgacaata
     8041 tttgcggcat ttttcacggc gttaccactt actgtcacaa tccgtgacac aatcggtgaa
     8101 ccttcaacaa gcgcatcatg gaccgcaata gcggtagtgc tgttattaac gataacaccg
     8161 atttcacttg gtaatttatc atattgtttt ttaaatatcg tttgaataag tgtgcgctcc
     8221 cagcccattg gataacgatc tggtacttca cgcacttcaa tgtcagggta aggggctgcc
     8281 gcttccttta agcaattgca agcttgttct ttttttactt taattgccac aataacttta
     8341 cttgctcccg cggctttttg taaataacga gcaccactaa taactttagc ggcattaaga
     8401 ctacaagaca cgtagtccgt tgttaaatac ggttcacatt caacagcatt aattaagata
     8461 gtgtgaatat ctttgacatt gttgtattta acccatgttg gaaaattagc gccaccaaga
     8521 ccgacaatac cagcttcttt aattgctgca acaatatctt cttttgatgc atctaattca
     8581 actttcttta atggtttttc tttagtcatt ttgaaatcat ttttaatgac taaatgatca
     8641 caaggacgtc ccgtagtaac attcaagcgt ttttcgattg ctcgaacagt tcccgagaca
     8701 ctactaaaaa gcggaacact aaattgctgt ttaagtgcaa tttttgttcc tacttttact
     8761 ttatcgcctt ctttgacgaa aacttcaagc ggcacattat tagcggctac aagcggaaaa
     8821 taaacatatt caactttttt agggtcgaga acaacaagtt cgcgttcaag actcatactt
     8881 ttatagccag gaattttccg ccgacctaca gtttggaata acttcatcac ttttccctcc
     8941 atcgcgcata ttatacacgc gtaaagagca aaaagtaaag taaatgtgta aatagttcac
     9001 ttttaagtga aacgaccata aataatgaat gtatgtttat ttatttctag ttaatttctt
     9061 aaaaattcca actgtttttg gaccaggttt atatctaaaa cgaaaatcaa aagcacggga
     9121 cttatgtaaa acagccttca tatgactaaa tgttttaaaa gtatgatacc cgtgataact
     9181 ccccatcccg ctattgccaa caccaccaaa ggggaagtgc ggatttgcta gttgcacaac
     9241 cgcatcatta atcgcgacag aaccacttgt tgtctctctt agaactttat tcattatttc
     9301 tttcgattta gtgaaaagat aaagcgctag cggtttagga cgttcattaa taaaagcaat
     9361 cgcttcatca agtgtttcat aagtaataat cggtaaaatt ggaccaaata tttcttcacg
     9421 cattagttcg ctatcaagtg gtggatttaa cacaactgtt gggttaatag tttgtgtttc
     9481 ttcatcaaaa ttatcactac ctacttcagt taaaacacct ttaagtcgca agtaatgctt
     9541 ttcattaata attttaggta accttgcttt ttcttcacct tcaacactaa atgcttgacg
     9601 aatatcatca actttatcaa taagtttttt atcaagtaac acatagtcag gcgctacaca
     9661 tgtttgtccg caattaataa gtttagcaaa tttaaggcgc cgaatcgtta aggggatatc
     9721 agcgtcttct gcaattatta acggtgactt tccgcctaat tcaaggacga ctggtgttaa
     9781 attttcactt gctttacgca tcaccgtttg tccgactcca ggactaccag taaagaaaac
     9841 gaaatcatac acaccttgta aaatttcttc atttacttta cgaccaccaa gaaataaaat
     9901 agcttcttta tttggaaaag cagtcgcaac aatttgctgc attactttcg caatttctgg
     9961 tgcgtaagca cttggtttta caaccgcata attgcctgcc gcaatcgctc caatcagtgg
    10021 taaaagtgtt agttgtaatg ggtagttcca tggactcata attaagactt gtccgtaggg
    10081 ctcaggtaca agaaaatttt tagcgggaaa ttgcacaaga aatgacttaa tcttttttct
    10141 tctcatccat ttttttagat tttttatggc aaaatgcatt tcttcataca caataccaag
    10201 ttcagtgaca aatacttccc attcatcttt atggagatct ttgtagaggg cttcatatat
    10261 gtgtttttca tataatttaa ctgctttttg caggtttatt aatagttctc gccgtttttc
    10321 ataacttaag ggaaagtttt ttttcattgc ttctctttgc gttttaattt gtttcaacat
    10381 taaatatttc tccttttaat tctcgataaa attcttctaa ctcactcgtc ttataaatac
    10441 gcggagtggg atagaggggg cgcgcttctt tgttagcata cactgctagt tgcggaatgt
    10501 cttcttcttt aatttctgga atcgttgtcg gtatttcaaa gtaagtattt aaagcacgga
    10561 ttttcgctat aaaaagttta gctgtttcac cgttacttaa tccttcttca acaaccccac
    10621 tcagccgtgc gagtcgcgct aattttcggt aaattcttcg tccatatttc tctagcataa
    10681 acggtaatat taaagcgtta acatagccat gcgctaaccc gtaatatgca ctaagcggat
    10741 gacttaaggc atgaacataa ccaacataat tacgcgtaaa agcaacccca gcatcataag
    10801 ctgctagttg catgtttttt cgtgcctcaa ggtcactagg attatcatat gcgactttga
    10861 gattttcaaa cactaatcgt gtagcgttaa tcgctttttc atttggaaat ttacgaccac
    10921 ccttatttgt gtaggcttca atagcgtgtg ttaaggcatc catccctgtc gtcgctgtca
    10981 caaattttgg taatttaaat gtgagtgtag gcactaataa tgcataactt ggaattaaaa
    11041 ctgggtcatt aatcgcatat ttgtgctttg ttctctcgtc aacaacaatc gacgctactg
    11101 tcgcttcact tcctgttcct gaagttgtgg gaacagcaat taaaagcggt gttttcttcc
    11161 gtacttttaa gagcccgcct aattgtttta aattcttccg tggtcgcgct acacgggcgg
    11221 caatggcttt agcaacatcc attggactgc cgccaccaaa accaataata gcgtcaccac
    11281 cataaataat gtaagcaagt agtccttctt caacttgggc tgtagtcgga ttagcggtta
    11341 tatcactaaa tacacgataa tttagttgtt tatttttaag ggcgtcaagt aagggttgat
    11401 gtaatttaag ttggactaaa ccttcatcca ttacaataaa aattcggctt ttattttgct
    11461 taattaaaac atcaactaat tcatcatacg cctttaaaat ttctggttca cgccatggta
    11521 aaatcgggct aaaggtacgc aatacttttt gaaatatacg gtcgcctaat tttgacatcg
    11581 catgcactcc tttccgctat agtgtttttg taatgatatt ttttaattga acttatgggt
    11641 ttaatttact tataacaata tttgtgattc ttctcatttt tattatagca aaaaagggac
    11701 ttggggtccc tttgatttgc ttttatctaa atggtgtcaa tctagaagat tggtacttcc
    11761 atcccgaaga gggcaaagaa ccacttaatc cactcccaga ccatgccgac aaatggattt
    11821 gatgttgggg tcataatatt aaagttaaca agtgcccaga cgataaccat taatatcgct
    11881 gcgacgaccg ctgcgactaa agcggcaata acaagcacta caattaatct ccataagaaa
    11941 cggaagaaac gacgagcgcg tgctcttctg acggcctttt tatcagcggg atcaacattt
    12001 aaatcatcac gacgtgaacg ccgtccatac ttcatttgat ttgatactag gctattaaca
    12061 aagttactat caaaatagac atttacggag tttttagcgg ctttctttgg cgtttgttgg
    12121 ccttctttgc cttcaacctt tgcttcgagc gctgctactt gagcttctaa ttctttaatt
    12181 ctacgttttt tactcataac tcaaatctct cctttctatc gtcctaatac tcggacttta
    12241 ccaaatagtc ctttaaataa atcaaagaca ctttggacag cgttgtggag ccataatacc
    12301 gcagcgttga aacgatcagc gagccatggt ggtcctgcaa gaggggtaat attgaaatgc
    12361 tctaatgtcc atgcacccat gaacacgact attgctaaga gcaagagaat aaagataatc
    12421 cagaatattg tccgtccaac actcttcttt ttacgttttt tacctttaac tgctttgcca
    12481 acatcaatcg cgtttttatt gtccgacatc cgtttagcaa ggttactatc aaaataaata
    12541 ttaacttctg tttcgggttg ttcttcttgc ggtgccggag tagctttttg attagctaat
    12601 tctttacgta agatttctag ttgcaattca tgaactttat ccgttaagcg atcaacttta
    12661 gcgtgaagtg aaccgtaata tggttcttca tattgcggca cttgttgcgg tgcaggcttt
    12721 ggttcaactg gtttttcttc aagcttctca atgtcttttt catcaagtaa tgctttaaca
    12781 cgaccatatt ccgctaaaac ttcatcgtat tcagtacggc taataagcat ggaccgttct
    12841 ttttcttgtt cagcaataat tgcttcgtag tgttcttttt gcttttgtaa ttcttttttg
    12901 ctaattactg tccgaattac tgttggtcct ggcacttcaa cgacgacttc ttttggtttt
    12961 tctgaaagct cagcctttgt ttgctttaat tctttagaaa gtgcttcaac tttttcttgt
    13021 tcttttaaaa gttcgctttc atcaaccttt gtttcatttt cgacaggtct ttcttctaat
    13081 tctttctcga gttccttaac gcgttcgcgc aacctgataa cttttggatt ttcatcacta
    13141 aaatcagtag cttcttcgac acgttctggt aattcatatt gtaaatcact aacgacattt
    13201 gccgcatttt tgtttacata gtttttaacg agtaattcac caacatcttg ttttttaagt
    13261 tcagttggaa catcaagttt atgcttgttc acaaattctt tcagttgtgc aacagtcata
    13321 tccttaacat cttccgttaa atgcgggaca tcttttgctt tcgctacttt tggtaaaaca
    13381 gcaataattc cgtccactat gtcctgtttt gtgatatttg ttggtagtaa aacatcattt
    13441 ttttcggcaa tttcacggat ttctttaatc gtggcactgt gtaagctctt ttccacaaat
    13501 gtgcgcgcta atacaccgtc gatatcgcct tcttcatcac cgaaaagccc ttcaagtgtc
    13561 gggaataatt ccttaagaac aactgggctt gtgctacttt tttgtgcttt cttataaagc
    13621 ttagcaagtt ctttgtcctt gcttacaact tgaatggcaa agagccataa gtcttcaaga
    13681 taataatcca ttagttcggg ttgaatcttg accatccgtt caaataatcc ttcaagaacg
    13741 gtgacagggc tatcttcaaa ataatcaagt cgactaaaga aatcatttga aagtcctttc
    13801 tccgcaacgc gtgagacaag tttaaacttc cagacttcat cacgcataac attctttaaa
    13861 atgtttgcat gatctcgacg tgaatataat ttagggccct ttaaaaattc gactaagtcg
    13921 cttactggta attttgccag tgttttgaga gcttcattaa caacgagctc ggttccatca
    13981 attacgatga aatctaatct ggttttaatt ttcatgttgt tttccccttt gctaacatta
    14041 tatcattgaa aagtgatgat gagtcaatga gttgaaatat ttttctcatt tttttgaatt
    14101 taggcgtgtt ttgtcgttaa aaatgaacaa aaagcactac ttacttagtg attttcgtgg
    14161 taaatagtgc ttataaaatt agttaagcga tgtagactgg agcagtgcta atctttgtcg
    14221 ctaagtcgta agtgctaacg tgataagttg aaaaaatata gagcactcct tcaattaaca
    14281 cggaacgatt aatcgggtaa acatattctt caactgctgg gtgacttatt ttaatgggtt
//
LOCUS       DAOSLP010000025        13841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_4517_length_13841_cov_7.732555, whole genome shotgun sequence.
ACCESSION   DAOSLP010000025 DAOSLP010000000
VERSION     DAOSLP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4517_length_13841_cov_7.732555"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1374
                     /gene="pheS"
                     /locus_tag="PK460_03305"
     CDS             343..1374
                     /gene="pheS"
                     /locus_tag="PK460_03305"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020060066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOE54142.1"
                     /translation="MDKRFESIKEEALSKINNAKSVKALNEVRNEYFAKSGLIATLMS
                     KIRELAPNERKDYGQAVNTLKVFLEEKLGAALREAQSNELSEKLESEKIDLTLPGRNL
                     ETAPQHPFHLVKDEICDIFIGMGYIIAEGDEVEEDLFNFTLLNMPENHPARDMQDTFY
                     INEKLLLRTHTSPVQARVMQKNKNQPLKMISPGKVFRRDDDDNTHSHQFGQVEGLVIG
                     EGISFSHLKATLDLFAKKMFGPKREIRLRSSYFPFTEPSVEVDVSCHVCNGKGCSTCK
                     GTGWIEILGAGMVNPNVLTMNGYDTEKFTGFAFGVGIERVAMLRYGIDDIRRFYQNDI
                     RFLKEFPKR"
     gene            1384..3768
                     /gene="pheT"
                     /locus_tag="PK460_03310"
     CDS             1384..3768
                     /gene="pheT"
                     /locus_tag="PK460_03310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOE54143.1"
                     /translation="MKISRNALERYIDLSTLSDKEIADGLTFAGIETEDYYKLASGTN
                     LVIGHVLTCIKVEGSDHLSYCTVDVGRLGTREIICGAPNVRSGQKVIVALEGAILPVG
                     EIKRVTIKGHNSEGMICSLSELGVPSELQSHDDLVGIHVLSEDAPVGNTQVLEYLGLD
                     DTIFDIRPLANRSDMLALYNVVRELGAIFDLHYNIPEIEVNPSFKTKLKLEVNTPLVP
                     IFALTEVRNVAIKPSPQWMATFLRKHGHRPVNNIVDIGNYVMLVTGRPLHMYDLDKVK
                     DEHFIVSSDATTKFIALDEKEYNIVPGDQIISDKEKVLCLGGINGAKSSAVTNETKRI
                     AIEVAAFDTAHIRMSVVRHNLPSEASTRFGKGVNLNNTTEAMNLALSLIFDLVPGAEF
                     SNTVTVEKSLPAQPVIYFDKDKINKLLGTTFSEEVMLSTLNRLNITVNEDKSVNLPLY
                     RQDFFSLADLAEEIIRLLGFEHVGNEIPALFTTVGSYDEVQRKRYDIRKLLRSKGLYE
                     VLTYALTDEEKNKTFTYLNKDEPLKLLHPMTPVRSELRLNLLPSVIETLTYNASRQQS
                     DFALFEISNITSENYKGEHLAFAFYGNEVLSRELSKRPYDFFDAKGLIISVLEYLGIN
                     ESRYEFVLNPHKNEEFHPAQSALLKVQGKVVGVFGKLHPLAKEKALLNNEAIIGELNL
                     SALLSLKTSLNKVNPPTRFPFVRRDLALLVNEDLSVKELINVIRKAGGKLISDVSVFD
                     VYTKLKDEPGKKSVALTLTLQDANKTLVEDEIKEVMTKVIEELTKKLQVVVRGI"
     gene            3765..4271
                     /locus_tag="PK460_03315"
     CDS             3765..4271
                     /locus_tag="PK460_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54144.1"
                     /translation="MKIKIADGIKLHQTLTAEVDLSDVDLHANYSLKAIKPFTVTVDF
                     DTFTDVVEVHIRGKVTLILECAYTLEHFDYEVEMDDELTFNFVNPNIEAETDDCFYEK
                     GPEIFLDPYLYAIILSYIPLKVIKPGANLPKSGEGYEILSEEEFEAKHQTLDDEFGDL
                     FDKLDLDD"
     gene            4361..4837
                     /locus_tag="PK460_03320"
     CDS             4361..4837
                     /locus_tag="PK460_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOE54145.1"
                     /translation="MYNVVKESGRKWEMKFFGTYYHSLDAKGRLVVPARFRETLKDLK
                     TLYILQGFDGAISVYPEEAYHQELSFLEAQNFKNADARAYIRTIYASIEELEVDAAGR
                     ITLPVRIQKKYDIGQKVIVIGVGDHLEIWDEERYLSYERDACGNLEDLAAKLQEKA"
     gene            4837..5781
                     /gene="rsmH"
                     /locus_tag="PK460_03325"
     CDS             4837..5781
                     /gene="rsmH"
                     /locus_tag="PK460_03325"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOE54146.1"
                     /translation="MSTHYPVLLNEVISGVNVKDDSIIIDLTLGRAGHSSALLFKARN
                     GYLYGFDQDETAIKESREILSKTFQNFTLFHQNFKDFDVALEALGVTGADIILLDLGV
                     SSPQFDVSDRGFSYRYEGPLDMRMDRTNNTLTAAIIVNTYSEQKLKEIFYRYAEHRYS
                     ARIAREIVNARNEQEIRTTTELVEIIKRALPSKELRKKGHPAKTIFQALRIEVNNELG
                     VLEKVLERAPHFLNEGGRLAVISFHSLEDRLVKNVFKKLATLSDEGLHPYTLPDKNRV
                     APFTLVNRKVIVATDEEIELNPRASSAKLRIIEKRRTK"
     gene            5781..5999
                     /locus_tag="PK460_03330"
     CDS             5781..5999
                     /locus_tag="PK460_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54147.1"
                     /translation="MRDKLYKTNKKVGYYRFRAFLIGILLVAVGAAALTIPYPYVAKA
                     INEAQARRAQAEEESSEEAPLVTEDLTI"
     gene            6155..7339
                     /gene="pilM"
                     /locus_tag="PK460_03335"
     CDS             6155..7339
                     /gene="pilM"
                     /locus_tag="PK460_03335"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOE54148.1"
                     /translation="MDIKISTLEISASAVKLVVGYELEGSPYVLYANKVALSQAISAG
                     EIVAPDQVINAIKVLAADAREALGETISEVVLALPTIDFEVFRGTQTTNTLSNKIDRI
                     DIKNIMALFRKNRVDPNKVIVAILPQVFKTDSDQVYRLLPLGEVSNTLTVEAFLHVLP
                     IWIYNSYIETVQKAGLKVRKVYPDKYALTELLNSPATATNTYFLVDFGAKQTSITFVS
                     NHNLYFSRIINEGSDDLTYLLAQKYGLELKEADRLKKDFGLDHRNVSVNFPLATGIGE
                     RGEEIKVFLSDIYSTCQTFLNSHLQKVLSEIKIVGKSQNMPNLNKYPLLFVGGGSTIY
                     NYEEIIGSDRSFPDKYIVKLDTIGARDLTYAVNLGLIKAYSKYAQDIGDEKASIGTLT
                     RS"
     gene            7342..8436
                     /gene="ftsZ"
                     /locus_tag="PK460_03340"
     CDS             7342..8436
                     /gene="ftsZ"
                     /locus_tag="PK460_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOE54149.1"
                     /translation="MIGTNDLDNFEPVARIVVIGVGGAGNNAVNRMIDDQIDSVSFWV
                     ANTDKQALATSKAKNRIVLGEEFTHGLGAGGEPSVGRKAAEASRPEIEKIVEGADLVF
                     IAAGMGGGTGTGASPIIAEIAKAAGALTIAIVTRPFTFEGKKRIANSVEGLNALKEIV
                     DSIIVVSNDKLLITGGNLPIAQAFSESDKVLAQSVKTVVDLILVPAVINLDFADVRNT
                     LLNSGIALIGFGMGKGKNKAHDAAQTAINSPLLEASLQGARKAIIAVTCGTNVSLFEA
                     QETVNEIVETSGSNLDVKFGVALNELLEDSIIVSVIASDFEEQYDFTSTHTSIIKPVT
                     PESRTYHAEDDEDDDSFLPNILKELEAEEN"
     gene            8707..11424
                     /gene="ileS"
                     /locus_tag="PK460_03345"
     CDS             8707..11424
                     /gene="ileS"
                     /locus_tag="PK460_03345"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOE54150.1"
                     /translation="MELKDTLLMPKTKFEMRGNLTRKEPGILARWEAIDLYKLMVKKN
                     ETSKPFILHDGPPYANGNIHSGHMLNRNLKDFVVRMKNMQGYDTPFIFGWDTHGLPIE
                     NQVTKSGVNRKAIPITEFRKLCKEYALKQVEIQKAQLKRLGLVGDFENAYLTLTKDYE
                     ADELRVFKRMALKGLIYRGLKPVQWSPSSETALAEAEIEYKDVVSDSIYVLFNVTKGN
                     DKVPVGTKFMIWTTTPWTLPANLAISVHPAFTYGVYESALGDVILLPELVEEVSKASD
                     LTFTLKYEVKGADLDLIETKHPLYERISLVITGMHVTSESGTGAVHTAPGHGLEDFVV
                     GQKYGLDVLCPVDARGYFTSEAGKYEGLFYEDANKVIINDLREVGALVAHTKVKHSYP
                     HDWRTDKPLIFRATPQWFASIEPIRRDLLNAVQKVDWYPAWGEGRISNMISDRGDWTI
                     SRQRVWGVPIPIIYNEDGSPILEEVVFDNIIAEVEKHGSNIWYEKDAKYFLPEGYQNA
                     LSPNGNFTKEVDTMDVWFDSGISAIHTLKKHGLPFPADLYLEGNDQYRGWFNSSLTLS
                     VAALEKAPYKAVVTHGFVVDDKGEKMSKSKGNVLDPSKLANEYGADILRLWSATIDYQ
                     EDARLSVDLLRQTSETYRKIRNTFRFMLGNLPALDTSFAELKALYKEPDFYLDKLMTN
                     KLQQVINKMIDSYNDYDFGAAFTTLMNFVTNELSSFYLDVAKDILYCEAKDSARRQSV
                     VGTLYKITVALLPLIAPVLVFTAEEIYEHLPVIKRLPSVHLMDFPYQLDVDNAKLEEF
                     SALLKVRSNAYQHIEKARQEGVLGSSQEASLTLPHSEVEVAPQLKKLDKEELARFFIV
                     SEVKFCNTYKVEKHTGHKCPRCWNYHDELVSVAEEQVCERCAKVLNTYE"
     gene            11417..11980
                     /gene="lspA"
                     /locus_tag="PK460_03350"
     CDS             11417..11980
                     /gene="lspA"
                     /locus_tag="PK460_03350"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOE54151.1"
                     /translation="MNKVKQYLKSFFYSYAWLAVLLLIIDQLTKVWALKAKLDITLIP
                     NFLHLTYVRNTGAAWSMFDGNMLALALISFVVGTLLIVARIKYRTKLDTFKKILFAVV
                     LAGTWGNFIDRAFYKLLTGAEGVVDYIHFQFGNYHFPVFNVADICITLGILSYVVLLL
                     VEEFKALKAPKTEEKVKDDTNEEKDGE"
     gene            11970..12884
                     /locus_tag="PK460_03355"
     CDS             11970..12884
                     /locus_tag="PK460_03355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE54152.1"
                     /translation="MENKLLKVDFEHSGKRADVVLSSLLSITRAQVQKMIKDGDIKLN
                     GTSVKASYRIEGGEEFSVTIREKEAPNLTPYEIELDVRYEDEYLLVVNKPAGLVVHPS
                     FGHKKETLVHALLARGDKYSDVNGVFRPGIVHRLDKETSGLLIVCKNNEVHNKIAKLL
                     KKREITRRYTALVRGKVREEAGVIKTYLTRSKDNFKKMANTPATGKLAISHFKVKERF
                     KGYMLLEVELETGRTHQIRAHLEFINTPIVGDALYGLKKDPLYSKGQLLHASYLAFTH
                     PYTGELVEVSAELPDYFTKVLRRLQKDL"
     gene            complement(12847..13779)
                     /locus_tag="PK460_03360"
     CDS             complement(12847..13779)
                     /locus_tag="PK460_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOE54153.1"
                     /translation="MHNYELRIPREDLGIVIEQTKLPSVSNKHSYMIIYYEKGNIKFS
                     IYDNTKNEPYKARFVGARLPKYLEPYLKEKDIKTPKSKSVTTKIVDKKITTFPHIGSD
                     EVGFGDFFGPLVVVAAYLDEELKALVESLNVKDSKALDDTKIVLLGNLLKDKIPHTKN
                     IVHNPKYNELIAQGYNMNQMKAMLHQNVLTTLAKRQKYDGKLYLDAFTSDKHYENYTA
                     LMSKAKVIQLKDGESNSLAIATASILARFYFLREIEVLNIRYNTKIPLGAGNAVDKFA
                     QEFLVKFGKNNLLSVVKQNFRNFKDLFADDGGLL"
BASE COUNT         4524 a         2354 c         2864 g         4099 t
ORIGIN      
        1 aaatttaaaa gtttcctaat ctttatttgt aaagataagt atgtcatcta acagtttaca
       61 ttttgtccta ttgccacatt cataattttg ccaatttata ttatattaag gtataatata
      121 tgcggcgttg aaaagaagta gtaatgtctt aaagttcttt agatagcgac ggtgagatgg
      181 tggaagcacc gctagagaag agacacgaat tcaccttgga gccggttgat gacccgttga
      241 ctgtcgttaa agtcgtaact aagagtggaa aattaggatg gtaccacggc gaaagtcgtt
      301 cctatgggga cgctttttta tttaataaaa ggagaaattc ttatggataa aaggtttgaa
      361 agtataaaag aagaagcttt aagtaaaata aataacgcta agagtgtgaa agcattaaat
      421 gaagtaagaa atgaatattt tgctaaaagc ggcttaatcg ctacgcttat gagtaagatt
      481 cgcgaactag cgcctaatga gcggaaggat tacggtcaag ctgtaaatac actaaaagtg
      541 tttcttgaag aaaaacttgg cgcagcactt agggaagcac aaagcaatga acttagtgaa
      601 aagttagaaa gtgaaaaaat tgatttaaca ctacctggaa gaaacctaga aacagcgccg
      661 caacacccct tccacctcgt taaagatgaa atttgcgata tctttatcgg gatgggctac
      721 attattgcgg aaggcgacga agttgaagaa gatctcttta actttactct tttaaatatg
      781 ccggaaaacc atccagcgcg tgatatgcaa gataccttct atattaatga aaagttatta
      841 cttagaacac atacttcacc ggtacaagca cgcgtaatgc aaaaaaataa aaatcaacca
      901 ctaaaaatga ttagtccggg gaaagtattc cgcagagatg atgatgataa tacccattcc
      961 catcaatttg gacaagttga aggcttagta attggggaag gcatttcttt tagtcactta
     1021 aaagcaacac ttgacttatt tgcgaaaaaa atgtttggtc cgaaacgaga aatacgctta
     1081 cgttcaagtt atttcccgtt tactgaacct agtgtggaag ttgatgttag ttgtcacgta
     1141 tgtaacggta aagggtgctc gacttgtaaa ggcactggtt ggattgaaat cttaggagcg
     1201 ggaatggtta atcctaatgt gttaacgatg aatggctacg atacggagaa gtttaccgga
     1261 tttgcctttg gtgtcgggat cgaacgcgta gcgatgctcc gctacgggat tgatgacatt
     1321 cgccgctttt atcaaaatga catccgtttc ttaaaggaat tcccaaaaag gtaggtaata
     1381 attatgaaaa tttcacgaaa tgcattagaa cgatacattg atttaagtac tttaagtgat
     1441 aaagaaattg cggacggact cactttcgcg ggaattgaaa ccgaagacta ttataaactc
     1501 gctagtggta cgaatttagt aattgggcat gttttaactt gcattaaagt tgaaggtagt
     1561 gatcaccttt cttactgcac ggttgacgtt ggtagattag gaacacgcga aattatatgc
     1621 ggggcgccta atgtgcgaag cggacaaaaa gttattgtcg cgcttgaagg agcaatttta
     1681 ccagtcggtg aaattaaacg cgtcacgatt aaaggacata atagtgaagg gatgatttgt
     1741 tcacttagcg aattaggagt gcctagtgaa ttacagtcgc acgacgattt agtaggtatc
     1801 catgttctaa gtgaagacgc accagttgga aatacacaag ttttagaata tttaggatta
     1861 gacgacacta tttttgatat tagaccactc gctaacagga gtgatatgct tgcgctatat
     1921 aatgttgtcc gtgagcttgg cgcgattttt gacttacatt ataatattcc ggaaattgaa
     1981 gttaatccgt cttttaagac aaaacttaaa ctagaagtta acacaccgct tgtgccaatc
     2041 tttgctttaa cagaagtaag gaatgtggca attaaaccat cccctcagtg gatggcaact
     2101 ttcttacgta aacacggaca cagacctgtt aataatattg ttgatattgg aaactatgtg
     2161 atgcttgtca caggtcggcc gttacatatg tatgacttag ataaagtaaa agatgaacac
     2221 tttattgtta gtagtgacgc cactactaag tttattgcgc ttgatgagaa agaatataac
     2281 attgttccag gggaccaaat cattagtgac aaagaaaaag tattatgttt aggcgggatt
     2341 aacggcgcta aaagttcagc agtcacaaat gagacaaaac gcattgccat tgaagtagca
     2401 gcttttgaca cggcgcatat tcggatgagt gtagtgcgtc acaatttacc aagtgaagca
     2461 agtacgcgct ttggtaaagg tgttaattta aataacacaa ctgaagcaat gaatttagcg
     2521 ctctcgctta tctttgactt agtgcctggt gctgaattta gtaatacagt tacagtcgaa
     2581 aagagtttac cagcgcaacc tgtaatttac tttgataaag ataagattaa taagttactg
     2641 ggcactactt ttagtgaaga agtaatgcta agtacactta accgtttaaa tattacggtg
     2701 aacgaagata agagtgttaa tctcccactt taccggcaag atttcttttc tctagccgac
     2761 ttagccgaag aaattattcg ccttttaggc tttgaacatg tcggtaacga aattcccgca
     2821 ttatttacga ctgttgggag ttatgacgaa gtacaaagaa agcgttatga tattcgtaaa
     2881 ttattacgca gtaaagggct ttatgaagtg ttaacttatg ccttaactga cgaagaaaag
     2941 aataaaacat ttacttactt aaataaagat gaacctttaa aattactgca cccgatgaca
     3001 ccagtcagaa gtgagttacg acttaatctt ttaccaagcg ttattgaaac acttacttat
     3061 aacgctagtc ggcaacaaag tgattttgcg ctttttgaaa ttagtaacat aacttcagaa
     3121 aattataaag gtgaacacct agcgtttgca ttctatggta atgaagtgtt aagtcgtgaa
     3181 ttaagtaaaa gaccatatga cttttttgac gctaaagggc tcattattag tgtgttagag
     3241 tatttaggga ttaatgaatc gcgttatgaa tttgtgctta atccgcataa gaatgaagag
     3301 ttccatcctg cgcaaagcgc actcttaaaa gttcaaggta aagtagttgg tgtttttggt
     3361 aaacttcacc cccttgcaaa agaaaaagca ttacttaata atgaagcaat tattggtgaa
     3421 cttaatttaa gtgcattgct tagtcttaaa acaagcttaa ataaagttaa cccaccgaca
     3481 aggttcccgt ttgtaaggcg tgacttagcg ctccttgtta atgaagactt aagtgttaaa
     3541 gaattaatta atgttattcg taaagctggt ggtaagttaa taagtgacgt cagtgtattt
     3601 gatgtttaca cgaaactaaa agatgaaccg ggtaaaaaat cagtagcgct gacacttacc
     3661 ttacaagacg ctaataagac attagttgaa gatgagatta aagaagtgat gactaaagtt
     3721 attgaagaat taacgaaaaa attacaagta gtggtacgcg gaatatgaaa ataaaaatag
     3781 ctgatggaat taaactccat caaactctaa cagcagaagt tgatttaagt gatgttgatt
     3841 tacacgctaa ttattcgctt aaagcgatta aaccttttac cgtaactgtt gactttgata
     3901 catttaccga tgttgtggaa gttcatatta gaggcaaagt cacacttatt ttagaatgtg
     3961 cctatacttt ggaacatttt gattatgaag tagaaatgga tgacgaactt acttttaact
     4021 ttgttaatcc gaatattgaa gcagaaacgg atgactgttt ttatgaaaaa ggaccggaaa
     4081 tctttttaga tccatactta tatgcaatta tcttaagtta tatcccgctt aaagttatta
     4141 agcctggcgc taatttacca aagagtggcg aaggttatga aatcttaagt gaagaagaat
     4201 ttgaagctaa acaccaaacg cttgatgatg aatttggtga tctctttgat aagctagatt
     4261 tagacgatta attattattc tcatagaatg agcataaaag aaggggcaac tcttcttttt
     4321 tattttttag tgttttttta tttacaacga aaggtcattt atgtataatg tagtcaaaga
     4381 aagtgggaga aagtgggaga tgaagttttt tgggacttat tatcacagtt tagacgctaa
     4441 gggacggctt gtcgtacctg ctcgttttcg tgaaacactt aaagacttaa aaacgcttta
     4501 catcttacaa ggctttgatg gggcaataag tgtgtatccc gaagaagctt accaccaaga
     4561 actttctttc cttgaagcac aaaacttcaa aaatgctgac gctagagcgt atattcgtac
     4621 aatttacgca agtattgaag agttagaagt agatgcagca ggccgtatta ccttaccagt
     4681 tcgtattcaa aagaaatatg acattgggca aaaagtaatt gtaattggcg tcggtgatca
     4741 tcttgaaatc tgggatgaag aacgctactt aagttacgaa agagatgcgt gcggtaactt
     4801 agaagaccta gctgctaagt tacaggaaaa agcctaatga gtacgcatta tccagtatta
     4861 cttaatgaag taataagtgg cgtaaatgtc aaagatgata gtatcattat tgacttaacc
     4921 ttaggacgag cggggcatag cagtgcctta ctcttcaaag cccgcaacgg ctatctctac
     4981 ggcttcgacc aagatgaaac tgcgataaaa gagtcgcggg aaatactcag taaaacattc
     5041 caaaacttca cgctcttcca tcaaaacttc aaagacttcg atgttgcgct agaagcactt
     5101 ggagtcacag gcgctgacat catcttactt gacttaggtg tcagtagtcc gcaattcgat
     5161 gtaagtgacc gcggctttag ttaccgctat gaaggtccac ttgatatgcg aatggaccgg
     5221 acgaacaata ccttaacagc agcgattatt gtcaacactt attcagaaca aaaactaaaa
     5281 gaaatattct accgctacgc ggaacatcgc tacagtgccc gcattgctcg cgaaatcgta
     5341 aatgcgagaa acgagcaaga aataagaaca acaaccgagt tagtcgaaat tataaaaaga
     5401 gccttaccaa gcaaagaatt acgcaagaaa ggccatcccg ctaaaacgat cttccaagca
     5461 cttcgcattg aagtaaataa cgaattagga gtacttgaaa aagtcttgga acgcgctcca
     5521 cacttcttaa atgaaggggg gagactcgca gtaataagct tccatagtct tgaagaccgc
     5581 ttagtaaaaa atgtctttaa aaagttagcg acactgagtg acgaaggact gcatccttac
     5641 acactacctg ataaaaatag agtagcaccc ttcacacttg tcaatcgtaa agtcatcgtc
     5701 gccactgacg aagaaataga actcaaccca cgcgctagta gtgcaaaact gcgaatcatc
     5761 gaaaaaagga ggactaaata atgcgtgaca aattatacaa aacgaataaa aaagtcggat
     5821 actaccgctt tagagccttt cttatcggaa ttttacttgt agcggttgga gcggcagctt
     5881 taacgattcc ttatccgtat gtcgctaaag cgattaacga ggcacaagca cgccgtgctc
     5941 aggccgaaga agaaagcagt gaagaagctc ctttagttac tgaagattta accatttagt
     6001 ttttttcctt tctttaaagc cttagtgaac tcttcccctt ttcctctctc actaaggctt
     6061 ttttcttaaa tttgtgataa ttaagcaaat attaattagt tgcaattgtt attattcgtt
     6121 aattattgta aaataattac attattggag aattatggat attaaaatta gtacattaga
     6181 aattagtgcc agtgccgtca aactcgttgt cggttatgaa cttgaaggta gtccctatgt
     6241 gctttatgca aataaagtgg cgctttcaca agcaataagt gcgggagaaa tagtcgcgcc
     6301 tgaccaagtc attaacgcga ttaaagtttt agcagcggac gctcgtgaag cattaggcga
     6361 aacgattagt gaagtagtgc tagccttacc aacgattgat tttgaagtgt ttagaggaac
     6421 acaaacgaca aacacgcttt cgaataaaat tgatcggatt gatattaaaa atatcatggc
     6481 acttttccgt aaaaaccgtg ttgatccaaa taaagtaatt gtggcgattt taccgcaagt
     6541 ttttaaaaca gatagcgatc aagtgtatcg cttactccca cttggtgaag taagtaatac
     6601 gctaacggta gaagctttct tacatgtttt accgatttgg atttataatt cctatattga
     6661 aacagtgcaa aaagctggac ttaaagtaag aaaagtttat cctgataaat atgccttaac
     6721 tgaactttta aatagtcctg caacggccac taatacgtat ttcttagttg actttggcgc
     6781 aaaacaaact tctattactt ttgtctctaa tcataactta tatttctcac gcattattaa
     6841 tgaaggaagt gatgacttaa cttacctttt agcgcagaag tatgggcttg agctaaaaga
     6901 agccgatcgg cttaaaaaag actttggtct tgatcatcgt aatgttagtg ttaatttccc
     6961 acttgcgacc ggaattggtg aacgcggtga agaaattaaa gtgtttttaa gtgatattta
     7021 ttcgacatgt caaacatttt taaatagtca cttacaaaag gtattaagtg aaattaaaat
     7081 tgtgggaaaa agtcaaaata tgccaaactt aaataaatat ccactgcttt ttgttggtgg
     7141 tggtagtact atttataatt atgaagaaat tattgggagt gaccgttcat tccctgataa
     7201 atatattgtt aaactcgata ccattggggc aagagactta acttacgctg ttaatttagg
     7261 actcattaaa gcttatagta agtatgccca agatatcggg gacgagaaag caagtattgg
     7321 gacactaaca aggagttaac tatgattgga acgaatgatt tagacaattt tgaacctgta
     7381 gctagaatag tagttattgg tgtcggtggc gctggtaata atgccgtaaa tcgcatgatc
     7441 gatgatcaaa tcgatagcgt atctttctgg gtggcaaata ccgataaaca agcacttgcc
     7501 acaagtaaag cgaaaaatcg tattgtgctt ggtgaagaat ttacacacgg tttaggtgct
     7561 ggtggtgaac caagtgttgg gcggaaagcc gccgaagcta gtcgtcccga aattgaaaaa
     7621 atcgttgaag gtgctgacct tgtctttatc gctgcaggta tgggtggtgg aacaggtaca
     7681 ggtgcctccc ccatcattgc tgaaattgcg aaagccgctg gtgctttaac aatcgcgatt
     7741 gttacgcggc cctttacctt tgaaggtaaa aaacgtattg ccaatagtgt tgaaggactt
     7801 aatgccttaa aagaaatcgt tgactccatt atcgttgtta gtaatgataa attacttatc
     7861 actggtggaa atctcccaat tgcccaagct tttagtgaaa gtgataaagt attagcgcaa
     7921 agtgtaaaaa cagttgtcga cttaatttta gtaccggctg tgattaatct tgactttgcc
     7981 gatgttcgta ataccctctt aaatagcggt attgccctta ttggctttgg gatggggaaa
     8041 gggaaaaata aagcgcacga cgcagcacaa acagcaatta atagtccgct tttagaagct
     8101 agtctccaag gggcaagaaa agcaattatt gctgtcacat gcgggacgaa tgtttcactt
     8161 tttgaagcac aagaaacagt taatgaaatc gttgaaacaa gcggatcaaa tcttgatgtt
     8221 aaatttggtg tcgcattaaa tgaattactt gaagactcca ttatcgtcag tgtcattgct
     8281 agtgactttg aagagcaata tgactttact agtacgcaca cttcaattat taaaccagtg
     8341 acaccagaat cgcgtactta tcacgctgaa gacgatgaag atgatgatag cttcttacca
     8401 aatatcttaa aagagttaga agcagaagaa aattaatgac attattatac tttaatgtat
     8461 aatagtagcg aaatcggacg aagacacggt ggcggtagaa ccttacaaag cgactaggga
     8521 aatggtgcaa gccctaagaa gaagctactc ccattatcac ttctgagtgc aactatgaac
     8581 aagagtaata gttgtcgctt aatctcacgt tacgagatac aaagtgctag attagtctag
     8641 aacacaggtg gtaccgcgga gtaattcgtc ctgtgactag acttttttta ttgaaggaga
     8701 gagactatgg aacttaaaga cacattatta atgccaaaga caaagtttga aatgcggggt
     8761 aacttaaccc gcaaggaacc aggtatttta gcgcgttggg aggcaatcga tttatataaa
     8821 ttaatggtga agaaaaatga aacaagtaaa ccttttattc tccatgacgg gccaccttac
     8881 gctaacggga acatccatag cggacatatg cttaaccgta acttaaaaga ctttgtcgta
     8941 cgcatgaaaa atatgcaagg ctatgacacg ccctttatct ttggctggga tacgcacggt
     9001 ctcccaattg aaaaccaagt cacaaaaagt ggggttaacc gtaaagcaat tccaattaca
     9061 gaattccgca agttatgtaa agaatatgca cttaagcaag tcgaaattca aaaagctcaa
     9121 cttaagcgtc ttggcttagt cggtgacttt gaaaatgctt atttaacttt gactaaagat
     9181 tacgaggctg atgaacttcg tgtctttaag cgtatggcac ttaaaggttt aatttatcgc
     9241 ggactaaagc ccgtgcaatg gtcaccaagt agtgaaactg cacttgctga agccgaaatt
     9301 gaatataaag atgtcgttag tgactcaatt tatgtgcttt ttaatgtaac aaaaggcaat
     9361 gataaagtac ctgttggcac taaatttatg atttggacaa caaccccgtg gacgcttcct
     9421 gctaacttag cgattagtgt ccatcccgcc tttacatatg gggtttacga aagcgcgtta
     9481 ggtgacgtta ttttactacc tgaacttgtc gaagaagtaa gtaaagcgag tgacttaact
     9541 tttacattaa aatatgaagt caaaggtgcg gatttagatt taattgaaac gaaacacccg
     9601 ctttatgagc gtatttccct tgttatcacc gggatgcatg tcactagtga aagcgggact
     9661 ggggcggttc acacggcgcc gggtcacggg cttgaagact ttgtggttgg gcaaaagtac
     9721 ggcttagatg tgttatgtcc tgttgacgct cgaggatact ttactagtga agctgggaaa
     9781 tatgaaggac tattttacga agacgctaat aaagttatta ttaatgactt acgggaagta
     9841 ggagcgcttg tcgcccatac gaaagtaaaa cacagttacc cacacgactg gcggacggat
     9901 aaaccgctta tctttcgcgc aacaccgcaa tggtttgcca gtattgaacc aattcgcaga
     9961 gatttactta acgctgtcca aaaagttgac tggtatccag cgtggggtga aggccggatt
    10021 agtaatatga ttagtgatcg cggtgactgg acaattagtc gtcaacgcgt atggggtgtc
    10081 cccattccga ttatttataa tgaagatggt agtccgattc ttgaagaagt agtatttgat
    10141 aacattattg cggaagtgga aaagcatggc agtaatatat ggtacgaaaa agatgcgaaa
    10201 tatttcttac cagaagggta ccaaaacgca ttatcaccaa acggtaactt tacgaaagaa
    10261 gttgacacga tggatgtgtg gtttgatagc ggcatcagtg cgattcatac acttaaaaaa
    10321 cacggtttac cttttcccgc tgatttatat ttagaaggta atgatcaata tcgtggttgg
    10381 tttaatagtt ccttaacttt aagcgttgcg gccttagaaa aagcaccata caaagccgta
    10441 gtgacacatg gctttgttgt tgatgataaa ggcgaaaaga tgtctaaatc caaaggtaat
    10501 gtgcttgatc caagtaaatt agcaaatgaa tatggtgcgg atattttacg cttatggagt
    10561 gcgacgattg attatcaaga agatgcacgg ctcagtgttg atcttttacg ccaaacaagt
    10621 gaaacttatc gtaaaatccg taatactttc cgctttatgc ttggtaattt acctgcgtta
    10681 gacacaagtt ttgctgaact taaagcatta tataaagaac cagactttta tcttgataaa
    10741 ttaatgacta ataaattaca acaagtcatt aataaaatga ttgatagtta taatgattac
    10801 gactttgggg cagcttttac tacactgatg aattttgtta cgaatgaatt aagtagtttc
    10861 tatcttgatg tggcgaaaga tatcttatac tgcgaagcta aagatagcgc tcgacgccaa
    10921 agtgttgtgg gaacacttta taaaattacg gtagcacttt taccactgat tgctcctgta
    10981 ttagtgttta ccgctgaaga aatttatgag cacttaccag tgattaagcg tttaccttcc
    11041 gtacatttaa tggatttccc ctatcaactt gatgtcgata acgctaaact tgaagaattt
    11101 agtgcgttac taaaagtacg aagcaacgcc tatcaacata ttgaaaaagc gcgccaagaa
    11161 ggagtacttg gcagtagtca agaagcaagc ttaacattac cacatagtga agtagaagta
    11221 gcgccacagt taaaaaaatt agacaaagaa gaacttgcac gcttctttat tgtaagtgaa
    11281 gttaagtttt gtaatactta taaagtagaa aagcacactg gtcacaaatg tccgcggtgc
    11341 tggaattatc atgatgaatt agtaagcgtt gctgaagaac aagtctgtga acgatgcgca
    11401 aaggtattaa acacatatga ataaagtaaa acagtactta aaatcattct tttattccta
    11461 tgcttggtta gccgtgctcc ttttaatcat tgaccaatta acaaaagtat gggcactaaa
    11521 ggcgaagtta gacatcacgc ttattcctaa tttcttacat ttaacatatg tgcgaaatac
    11581 aggcgcagcg tggagtatgt ttgatggtaa catgctcgct ttagcactga ttagttttgt
    11641 tgtcggtacg ctgcttattg tagcgcgcat taagtatcgc acaaagttag atacatttaa
    11701 gaaaatactt tttgcggttg tccttgccgg aacttggggt aattttattg accgcgcttt
    11761 ctataaactt ttaacaggtg cagaaggtgt tgttgattat attcatttcc agtttggtaa
    11821 ttatcatttc cctgtgttta atgtcgctga tatttgtatt actttaggga tcttaagtta
    11881 tgtcgtttta ttattagtgg aagagtttaa agcgctaaaa gctccaaaaa ctgaagaaaa
    11941 agttaaagac gacactaatg aggaaaaaga tggagaataa acttttaaaa gttgattttg
    12001 agcatagtgg taagcgcgct gatgtagtgc tttcttcttt attaagtatt acgcgcgcgc
    12061 aagtgcagaa aatgattaaa gacggggaca ttaaacttaa cggcaccagc gttaaagcaa
    12121 gttaccgtat tgaaggcggc gaagagttta gtgttactat tcgcgaaaaa gaagcaccaa
    12181 acttaacacc ctacgaaatt gaacttgatg tgcgttatga agacgaatat ttgctcgttg
    12241 tcaataaacc cgcgggatta gtcgtgcatc ctagttttgg tcataaaaaa gaaactttag
    12301 tgcatgccct tttagcgcgc ggtgacaaat atagtgatgt taacggtgtt tttcgtcctg
    12361 gtattgttca tcgccttgat aaagaaacaa gcggtcttct tatcgtatgt aaaaataatg
    12421 aagtgcataa taaaattgct aaactcttaa agaagcgtga aatcactaga cgatacacag
    12481 cgcttgtgcg cggtaaagtg cgtgaagaag cgggtgtaat aaagacatac ttaacacgca
    12541 gtaaagacaa ttttaaaaag atggctaata ctcccgcaac tggtaaacta gcgattagtc
    12601 actttaaagt taaagaacgc tttaagggct atatgctttt agaagtggaa ttagaaacag
    12661 gccgtactca tcaaattaga gcgcacttag aatttattaa tacgccaatt gttggtgacg
    12721 cgctttacgg tttaaaaaaa gacccgctat atagtaaggg tcaattatta cacgctagtt
    12781 atttagcgtt tacgcatccg tataccggag aattagtaga agtaagtgca gagcttccag
    12841 attattttac aaaagtcctc cgtcgtctgc aaaaagatct ttaaaattac ggaagttttg
    12901 tttcacgaca cttaataaat tatttttacc aaatttaacc aaaaattctt gcgcaaattt
    12961 atcaacagca tttcccgctc caagcgggat ttttgtatta taacgaatgt ttaatacttc
    13021 aatttcacga aggaagtaga agcgcgctaa tatactagca gtagcaatcg ctaatgaatt
    13081 actttctccg tcttttagtt ggatgacttt tgctttactc atcagtgccg tatagttttc
    13141 gtaatgctta tcactcgtaa aggcgtcaag atacagttta ccatcgtatt tttgccgttt
    13201 agcaagtgtc gttaacacat tttgatggag cattgctttc atttggttca tattgtaacc
    13261 ttgtgcgata agttcattat atttcgggtt atgcacaata tttttagtgt gggggatttt
    13321 atcttttagt aaattaccaa gtaagacaat ttttgtatca tcaagcgctt ttgaatcttt
    13381 cacattaaga ctttccacta gggcttttaa ttcttcatct agataagcgg caacaacgac
    13441 aagtggacca aagaagtcac caaacccgac ttcgtcactg ccaatatgcg ggaaagttgt
    13501 tatcttttta tcgactattt tagtcgtaac gcttttactt ttaggcgttt taatatcttt
    13561 ttcttttaag tacggttcaa gatatttagg taaacgcgca ccaacaaaac gcgctttata
    13621 tggttcattt ttcgtattat cataaattga aaacttaatg tttccttttt cataataaat
    13681 aatcatatat gaatgtttat ttgaaacact aggtaatttt gtttgttcaa tcacaatacc
    13741 taaatcttca cgcggaattc gtaattcata gttatgcatg agtatatttt aacataaacg
    13801 acaatcttgt tcgttattag aaaataatca ttaatgataa a
//
LOCUS       DAOSLP010000026        13502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_4659_length_13502_cov_13.311594, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000026 DAOSLP010000000
VERSION     DAOSLP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13502
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4659_length_13502_cov_13.311594"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /locus_tag="PK460_03365"
     CDS             <1..1944
                     /locus_tag="PK460_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOE54154.1"
                     /translation="KIKYFGNEELSIPMEQIHLIRKYSGREGVAPRLHKIGSPVWQRT
                     KVEISKKIEDIAEELIAIQAARYQRPGFAFPPDDEFQKEFELGFPYELTYDQEIALRE
                     IKNDMESPLIMDRLLSGDVGFGKTEIALRAAFKAISAGKQVMFLCPTTLLARQHYEVS
                     LERFANFGVKIAMISRLVTQKEKDEIVKDFQAGNIHLLIGTHALFNIDVESVDLGLLI
                     VDEEQRFGVEQKEIIKKMKTRVDVLTLSATPIPRTLQMSLVGIRGLSQINTAPQERMP
                     IQTYVIKRDDYVIKELVERELGRGGQVFYLYNRIATIYHVANKLSRLVPQAKIGVVHG
                     KMDRADIEDVMTSFYNNEINVLVATSIIENGIDVPNANVIIVENADTFGLSQLYQIKG
                     RVGRGNRIAYAYLLYQGNKIMNDDAKKRLQAIQEFTDLGSGYKIAQRDLMIRGAGDIL
                     GAEQSGFIDSIGIDMYLKMLEDAINEKKYKLTSDPIEKTINISGLEAYIPDDFASKSE
                     KFDIYLEIKKSKKMKELKDFEEKVIDMYGKLPRSLEILFLKRQVELVVNSRANFIEDI
                     KDEDVFIDIIPGRAFLDIPGSVVMLNNEIGTTYQNIRFITQDDKIKLRIIKRGDWFHA
                     YASIVKAIVMIVRETMKNKDETR"
     gene            1982..2191
                     /locus_tag="PK460_03370"
     CDS             1982..2191
                     /locus_tag="PK460_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54155.1"
                     /translation="MAKDISTAGKVIVDKRTVKPSYTVKVDDVLELYLGAHIVVVKVL
                     TVDEKEIKSNPEKGYELLSRTKNAN"
     gene            2181..3407
                     /gene="tilS"
                     /locus_tag="PK460_03375"
     CDS             2181..3407
                     /gene="tilS"
                     /locus_tag="PK460_03375"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOE54156.1"
                     /translation="MQINLDHNKKYLLACSYGPDSMALFSLLLKGNYNFAVAHINYGL
                     RGEESEKETNDLALYCKEHNIAFFVKWVNGKKIKGNFQNEARKVRYEFFEEIIKEHDF
                     DALLTAHHADDVIETYLMQQNSKRETFYYGIREEIVLNNFLVIRPLLNYYKNDLVFYC
                     VEHKVPFAVDSSNLELTYTRNKIRHNLVKKMSVEQKDKLLLDIKGRNKDLKAMTNKLE
                     LLLENSTISITEFIKLNNREQQHLLYMLFHNKGIADKFSKGRNNQIKDVVLSDKNSAM
                     IKVIDSYYLVKYEDKFSLININNYQPYKYIVETPETIKTPQFVAFFGKNNVFPNIPAS
                     EYPLTIATQGKNQKYKIKGYEKTINRLFIDMKMPRHYRLIWPVVTNKNGEVIYVPRYR
                     ANYIPKATDLFKIYIK"
     gene            3510..5495
                     /gene="ftsH"
                     /locus_tag="PK460_03380"
     CDS             3510..5495
                     /gene="ftsH"
                     /locus_tag="PK460_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOE54157.1"
                     /translation="MSNPNPRPNPRSVPPRRSLRTLLPYLLLFAVIGVVIWLAFFTGN
                     AKTQALLQTELAAYLQTNAGVIDTITIVTTETTTKITGILKDGSKFTALVDRTFYENI
                     LQPTLFAADGTALVDFKMVIVNAYQRTFWNTIGPSLLYLVLFVVAMIFLYSRMAGAVS
                     SSNTKAMEFNRSRARLETKVKKTFDDVAGADAEKQEMMEIVDYLRNPKKYTKFGAKLP
                     KGVLLVGPPGTGKTLLAKAVAGEANVPFYSISGSDFVEMFVGVGAGRVRDMFQQAKKT
                     APCLIFIDELDAVGRQRGAGLGGGNDEREQTLNQLLIEMDGFSDNSGIIVLAATNRDD
                     VLDPALLRAGRFDRKITVSLPDRAGRTAIFGVHARGKIIDESIDFDAISRRTVGFSGA
                     DIESVLNDAAILAIRDNADKIMLQHIDEAIDRRIAGPARTSRQLNEHERKVIAYHEAG
                     HAVVGLNHPFSDKVQKITIIPRGQTGGHVLMTPEEDRFLMTKNQLIARIVGYLGGRTS
                     EEVFFDDVSTGAQNDIEVATRIARLMVTEFGMSDLGPIQYERDTGSVFLGRDYTSSQK
                     NFSQQVALEIDKEVRKIIEQAHDKAREIIEKHKDDVILIAETLLDRETITAEQIDRLL
                     KDRKLDEPKQAVIGEVVLTEKPKVEPTPTPVPEDDKK"
     gene            5534..6514
                     /gene="dusB"
                     /locus_tag="PK460_03385"
     CDS             5534..6514
                     /gene="dusB"
                     /locus_tag="PK460_03385"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOE54158.1"
                     /translation="MWKIGNIEIKGKTILAPMAGITTFAYREFMRSFGVALCYTEMIS
                     DSGLIFNNEKTYEYLPRPEEKRPTSVQLFGGETTKLLVAIEKIEKHTDNYDFLDLNLG
                     CPVPKVTRNNGGSAWLKHPNELIAMVEKVVKRSTKPVTVKIRIGWDEKHINFKELLPK
                     LEEAGVSLIAIHLRTTKQLYGGKARYDLAQNIQNLVSIPLVVSGDIYTLDDAIKALEI
                     TGAQAVMLARGQLGNPFLATQIETYFKTGERLKDPSLYQQLDYLWTLANMMVEEKGEH
                     MGILVMRGIAHHFIKGFPFTKTYKVKLTKMSTLNDLELIIKEIKNQALLI"
     gene            6555..8039
                     /gene="lysS"
                     /locus_tag="PK460_03390"
     CDS             6555..8039
                     /gene="lysS"
                     /locus_tag="PK460_03390"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOE54159.1"
                     /translation="MPREFSDQELVRRAKAEELSAQGIDPFGKKYERTDTSLSIKEKA
                     GEFTKEELEEKAIKVKTAGRIMLMRIMGKASFFTIQDQEGRIQAFISKQGVGEEVYDL
                     FKSADIGDIVGVEGTIMRTKTGEVTVKVEVYTHIVKALRPLPEKFHGLTDVEERYRRR
                     YVDLIMNESSKHVALTRPKIIRAIQKFLDDRGFVEVETSMLNSVLGGASAKPFITHHN
                     TLNRDFYLRIATELPLKRLLVGGIEKVYEIGRLFRNEGMSTRHNPEFTTVEAYQAYAD
                     LDDMYELCESLVRELAIKVTGDTKLPWGDEIIDVGAPFRKVSMVDLVKEKTGVDFRKI
                     KTFEEAKAIADKHEIHVEKHWTGSGHILNAFFEEFCEADLIQPTFVYGHPLEISPLAK
                     KAEDPRFTERFELFIKANELANAFTELNDPRDQYERFKAQLKARELGDEEASEMDEDF
                     VEALEYGMPPAGGIGIGIDRLVMFLLNQTSIREVILFPTMRDNK"
     gene            8188..8475
                     /gene="rpsF"
                     /locus_tag="PK460_03395"
     CDS             8188..8475
                     /gene="rpsF"
                     /locus_tag="PK460_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOE54160.1"
                     /translation="MKKYEIMYILLADLSEEERKAEMESLHQMITNLGGKIVDVDTEN
                     WGLRDFAYPINDLLKGYYVVINVESDAAAIAEFERLSKLNKNVIRHLVIAK"
     gene            8494..8907
                     /gene="ssb"
                     /locus_tag="PK460_03400"
     CDS             8494..8907
                     /gene="ssb"
                     /locus_tag="PK460_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014645360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE54161.1"
                     /translation="MINRVVLVGRLTHDPELRKTSSGASVTNFSIAVNNRFARDQENS
                     TSFFRVSCWNQTADFVAQYARKGNLVGVDGRIEQRSYVNNAGQKVNTVDIIADSVQLL
                     ESKSESSEGTGYQSDAEPATDLGEGIDISDDDLPF"
     gene            8932..9159
                     /gene="rpsR"
                     /locus_tag="PK460_03405"
     CDS             8932..9159
                     /gene="rpsR"
                     /locus_tag="PK460_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOE54162.1"
                     /translation="MSYRKSRPRRKVCYFKQNKITEIDYKNVELLKRFVTPNGKITPR
                     RVTGTSAKYQRQLAVAIKNARFMGLLPYYVS"
     gene            9214..10167
                     /locus_tag="PK460_03410"
     CDS             9214..10167
                     /locus_tag="PK460_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54163.1"
                     /translation="MKKTKFYYFELGLVILITLGLYVFSTNFLLLHYIGFLGPVPLAV
                     FIIRHKIKDGIFPAIFMILMGTLIGHFLPVGQGSFMRGFLFMIYAMTVGFLHGTISKT
                     KINPLIEILIIIGVEIFYGFLLVIVFYVQKDPHFAYDIQFGQYLELFIKLFKIDETSN
                     YAQNVGVIIKNGVIAYTIVFSFISVLLTHILIHLTRKFIYREKMEHGPLHGLEFTISR
                     VAAITYLVGCVVVLVLSFLLTKELNLLGISSINAIFTVFFSVTIIFVFQGILAVNLRL
                     EAKGCNNLSTLVFISGILLSPIFAIIGAINPLLPRKEKAVV"
     gene            10249..10845
                     /locus_tag="PK460_03415"
     CDS             10249..10845
                     /locus_tag="PK460_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54164.1"
                     /translation="MARKKKNDVALYDDSSYEEDDLKYAADEEVYAESLEDDEGKAKK
                     KRKKKKKEPKDPKERKDGSETRGGKTLTDRYHYRGRYYLLPERELRKQLRFYQKVFNY
                     RFLGIIAGVIALIFSILSLTFNAYFAVFTLLIVITGLIYGLLGMLKLRGLNWLFVLIG
                     IGLNIMAIVLAMKPLTFVFKHLGEIMALINEYMSSAGS"
     gene            10967..13054
                     /locus_tag="PK460_03420"
     CDS             10967..13054
                     /locus_tag="PK460_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOE54165.1"
                     /translation="MIKAITKLRKRTIILVIVEGLLLGLISLFYYLNLFNFQEIFVPP
                     VIFSVAIGVVFFNLFVFIFITARINKLRKKNTFELVNLLGGDVKEAYNFGMLGLVVVD
                     NDFNVTWASELFKERRLLIIDKNILAWKPELIRLVELSEVLADNVEDTIKVEIESRNY
                     EVKYLSKAGIFLFKDITEFEYLFDSNQATAPVLGVVVIDNFVDIVVKGEHSNPIFGTV
                     NSLILEYFSNYGVLLKQYREDAYLLILNKENFDKLYADNFSILQKIKDKTSNQDYQIS
                     LSMAFAYNFPDVLKLNELVLNAIDTAMARGGDQVVIARYGEESEFIGGATSQAEKRNK
                     TAIRLYADTIFRHIREAKNVLIMTHTDTDFDALGASLGLKAMADYVKKTQKGTKVQFQ
                     PARIVYEHNLTELKTRMAVNRMFTKQEMNDMFISPEEIDDQKGTASLVNSGTLLIIAD
                     VSCPQLTLHPKLLERVDKVIVIDHHRRTDEFIDQRLYDYIETSASSTCEIVTELLHYG
                     NTPNFVLPPKYATFMLAGIYLDTNYFRTKTTGSRTFLASMVLEDWGADVMTADDFLKD
                     NVEEYALITKIMNSMTTLSVGIVFLRADETDIVTPETLSKVANQSMRIKGIQASFVIG
                     YTAPKVVKVSGRSNGEINVQFLLEKVGGGGHQASAAAVFTDKTLDDVQEILEDVINQN
                     LSQAKVVGGKKGG"
     gene            13056..>13502
                     /gene="rplI"
                     /locus_tag="PK460_03425"
     CDS             13056..>13502
                     /gene="rplI"
                     /locus_tag="PK460_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOE54166.1"
                     /translation="MKVILLKDVKKVGKKDEIVDVSDGYATNFLIPRKLAVPYNERTI
                     SQLKANQARALAEEKAHKEEALVNKKKLEEKEISFILAIGEKGKAFGAITAKKVEEKI
                     KEEFKINVDRRKFVNFQPLNQLGPTIVEIELFKDVIAKVKVLVKDKE"
BASE COUNT         4708 a         2132 c         2614 g         4048 t
ORIGIN      
        1 aaaattaaat attttggcaa tgaagaatta tcaattccaa tggaacaaat tcatttaatt
       61 cgcaaatata gtggccgaga aggcgttgca ccgcgactgc ataaaattgg ttcaccagta
      121 tggcagagaa caaaggttga aataagcaaa aaaattgaag acattgctga agaacttatt
      181 gctattcaag cagcgcgtta tcaaagacca ggttttgctt ttccgcctga tgacgaattt
      241 caaaaagaat ttgaacttgg tttcccatat gaattgacct acgaccaaga aatagcgctt
      301 cgggaaatta aaaacgatat ggaatcacca cttattatgg accgacttct ttcgggtgat
      361 gtaggctttg gtaaaacaga aatagcactt cgcgccgcct ttaaagcaat aagcgctggt
      421 aaacaagtaa tgtttttatg ccccacaacg ttacttgcaa gacagcacta tgaggtgtca
      481 ctagagagat ttgctaattt cggtgtaaaa atagcaatga tttctcgtct tgttactcaa
      541 aaagaaaagg atgaaatcgt aaaagacttt caagctggca atattcattt gcttattggt
      601 acacatgctt tgtttaatat agatgttgaa agcgttgatc ttggattatt aattgtcgat
      661 gaagaacaaa ggtttggcgt tgaacaaaaa gagattatta aaaaaatgaa aacaagagtc
      721 gatgttttaa cattgagcgc cactcctatt ccgcgtacat tacaaatgtc attagttggc
      781 atccgcggct taagtcaaat taatactgct ccccaagaaa gaatgccaat tcaaacttat
      841 gtcattaaaa gagatgatta tgtaatcaaa gaattagtgg agcgcgagtt agggcgtggc
      901 ggacaagtat tttatttata taatcgtatt gcaacgatat accatgtcgc aaataagtta
      961 agcaggctag tgccacaagc taaaattggc gttgtacatg ggaaaatgga tcgcgctgat
     1021 attgaagatg taatgacaag tttctataat aacgaaatta atgttttagt cgctacttct
     1081 attattgaaa acgggattga tgtgccaaat gcaaatgtaa tcatcgttga aaatgctgat
     1141 acttttggac tatcgcaact ttatcaaata aaaggaaggg ttggaagagg aaaccgcatt
     1201 gcatacgcat atttacttta tcaaggcaac aaaataatga atgatgatgc gaaaaaaaga
     1261 cttcaagcaa ttcaagagtt tacagattta ggcagtggct acaaaatagc acaacgcgat
     1321 ttaatgattc gtggtgccgg tgatatttta ggtgcggaac aatctgggtt tattgatagt
     1381 atcggtattg atatgtattt aaagatgctt gaagatgcaa taaatgagaa aaaatataaa
     1441 ctaacaagtg atccaataga aaaaacaatc aatattagcg gattagaagc ttatattcct
     1501 gatgattttg ctagtaagtc agaaaaattt gatatttatc tcgaaattaa aaaaagtaaa
     1561 aagatgaaag aactaaaaga ctttgaagaa aaagtaatcg atatgtatgg taaattacca
     1621 cgttcattag aaatcttatt tttaaaacgc caagttgaac tagtggtaaa tagtcgcgct
     1681 aactttattg aagacattaa agatgaagat gtatttattg atattattcc aggaagagca
     1741 tttttagata tccctggctc agtagtaatg ttaaataatg aaatagggac aacataccag
     1801 aacattcgtt ttataaccca agatgataaa attaagttaa ggataattaa aagaggagat
     1861 tggtttcacg catacgcgtc aattgttaaa gcaattgtca tgattgttcg tgaaacaatg
     1921 aaaaataaag atgagactag ataaatttct aaaattgacg cgattaatta aacgaagaac
     1981 ggtggcaaaa gatataagta cggccggaaa agttatcgtt gataaaagaa ctgttaaacc
     2041 atcatacact gttaaagttg atgatgtttt agaattatat ttaggagcac atatcgttgt
     2101 cgtaaaagta cttacggttg atgaaaaaga aataaaaagt aatccagaaa aaggatatga
     2161 attattaagt agaacaaaaa atgcaaatta atcttgatca taacaaaaaa tacttattag
     2221 cgtgcagcta tggtcctgat tctatggcgc ttttttcact attgctaaaa ggaaactata
     2281 actttgcagt tgcgcatatt aattacggat taagaggcga agagagtgaa aaagagacta
     2341 acgatctcgc actttattgc aaagaacata atattgcttt ttttgtaaag tgggttaacg
     2401 gtaaaaaaat caaaggtaac ttccaaaacg aagcaagaaa agttcgttat gagttttttg
     2461 aagagattat taaagaacac gattttgatg cactattaac agcgcatcac gctgatgacg
     2521 tgattgaaac atatttaatg cagcaaaata gtaaacgtga aacattttac tatggaatta
     2581 gagaagaaat tgttctaaac aactttcttg ttattcgtcc gttactaaac tattacaaaa
     2641 atgatctagt gttttattgt gtcgaacaca aagttccttt tgcagtagac agttcaaatt
     2701 tagagttaac atacaccaga aataaaattc gccacaactt agttaaaaaa atgtctgttg
     2761 aacaaaaaga caaactactc ctagacatta aagggcgcaa taaagattta aaagcaatga
     2821 caaataaact tgaattattg ttagaaaata gtactatttc cataacggaa tttattaaac
     2881 taaataatag ggagcaacag catcttttat atatgctttt tcataataaa ggaattgcag
     2941 ataagtttag taagggcaga aataaccaga ttaaagatgt tgtgttaagt gataaaaatt
     3001 cagcaatgat taaagtaatt gattcatatt atttagtgaa gtatgaagat aaattttcat
     3061 taattaatat caataattat cagccttaca aatatattgt tgaaacacca gaaacaatta
     3121 aaaccccgca attcgtggct ttttttggca aaaacaatgt atttcctaat attcctgcta
     3181 gcgaatatcc tttgacaatt gctacacaag gaaaaaatca aaagtataaa atcaaaggtt
     3241 atgaaaagac aataaatcgt ttatttattg atatgaagat gccgcgtcac tatcgactta
     3301 tttggccggt tgttacaaat aaaaatggtg aagtaattta tgtcccgcgc tatcgcgcta
     3361 actacattcc aaaagcaacg gatttattta aaatttatat taaataaaac gcaaatgttg
     3421 aaatctaaga ttatactctg tataataact ttcagtgttt atttatgtta cacatattcg
     3481 aaattaatct ataacccaag gaggtcttaa tgagcaatcc aaatccacgt ccaaacccta
     3541 gaagcgttcc accgcgtcgc tcgctgcgaa cccttttacc ttatttactt ttatttgctg
     3601 taatcggtgt tgttatctgg ttagcatttt ttacgggaaa cgctaaaact caagcactac
     3661 ttcaaaccga actagcagca tacttacaaa caaatgctgg tgttattgac acaattacta
     3721 ttgtaactac cgaaaccacc acgaaaatta ctggtatttt aaaagacggt agtaagttta
     3781 ccgctttagt agaccgaaca ttttatgaaa acatcttaca accaacatta ttcgccgctg
     3841 atggaacggc gcttgttgac tttaagatgg ttattgttaa tgcttatcaa cgcacatttt
     3901 ggaacactat tggaccaagc ttactttatc ttgtcctatt tgtagtggca atgatattcc
     3961 tttactcaag aatggctggt gcagttagca gttcaaatac taaagccatg gaatttaatc
     4021 gttcacgcgc tcgcttagaa acaaaagtta aaaagacgtt tgacgatgtt gcaggcgctg
     4081 atgctgaaaa acaagagatg atggaaattg ttgattattt acgtaatccc aaaaaatata
     4141 caaaattcgg tgcaaaacta ccaaaaggtg tacttcttgt ggggccgcca ggcaccggta
     4201 aaacactttt agctaaagca gtagcaggtg aggctaatgt gcctttctac tcaatttcag
     4261 gttctgactt tgttgaaatg tttgttggtg ttggtgcagg tcgcgttaga gacatgttcc
     4321 aacaagcaaa gaaaacagca ccttgcttaa ttttcattga tgaattagac gctgttggtc
     4381 gtcaacgtgg tgccggactt ggtggtggaa atgacgaacg cgaacaaacg cttaaccaac
     4441 ttttaattga gatggatggt tttagtgata actcaggaat tattgttctt gcggcgacaa
     4501 accgcgatga cgttttagac ccagccttat tacgtgcagg acggtttgac cgtaaaatta
     4561 ccgttagtct accggatcgc gctggtagaa cagcaatctt tggtgttcat gctcgcggta
     4621 aaattattga tgaatcaatt gactttgatg ccatttcacg ccggactgtt ggattttcag
     4681 gcgcagatat tgaaagcgtg ttaaatgatg cagcgatctt agcaattcgc gataatgctg
     4741 acaaaattat gttacagcat attgatgaag ctattgatcg tcgtattgct ggtcctgccc
     4801 gcacaagccg acaacttaat gaacacgaac gcaaagttat tgcttatcac gaagcgggtc
     4861 acgctgttgt ggggctaaat cacccattta gtgataaagt gcaaaaaatt acaattattc
     4921 cacgtggtca aactggcggt catgtactta tgactcctga agaagaccgc ttcttaatga
     4981 ctaaaaacca attaattgct cgtatcgtcg gttatttagg cggtcgtaca agtgaagaag
     5041 tattctttga tgatgtatca acaggcgcac aaaatgatat tgaagtcgct acaagaattg
     5101 ctcgactgat ggttactgaa tttggtatga gtgatttagg tccgattcaa tatgaaagag
     5161 atacaggcag tgtcttttta ggacgcgatt acacttcaag tcaaaagaac tttagccaac
     5221 aagttgcgct tgaaattgac aaagaagtac gcaagattat tgaacaagcc catgataaag
     5281 ctcgggaaat tattgaaaaa cataaagatg atgttattct tattgccgaa acattacttg
     5341 accgcgaaac aattacggca gaacaaattg atcgtttgtt aaaagatcgt aaactcgatg
     5401 aaccaaaaca agcagtgatt ggtgaagttg ttttaactga aaaaccaaaa gttgaaccaa
     5461 caccaacacc tgttccggaa gacgataaaa aataagaaat cgaaagccga agcaattcgg
     5521 ctttttaata acaatgtgga aaataggaaa catagaaatt aaaggtaaaa ccattcttgc
     5581 tccaatggcg ggcataacga cttttgctta tcgcgaattt atgcgctcct tcggtgtagc
     5641 gctttgttat accgaaatga taagtgatag cggccttatt tttaataatg aaaaaacata
     5701 tgaatattta ccacgaccag aagaaaaaag accaacatcg gtgcaacttt ttggcggtga
     5761 aacaactaag cttttagtgg caattgaaaa gattgaaaag cacacggata attatgattt
     5821 tcttgattta aatttaggat gtcccgtacc taaggtaaca cgaaataatg gcggttctgc
     5881 atggttaaag catccaaacg agcttattgc tatggttgaa aaagttgtta aaagaagcac
     5941 aaaaccagtg actgttaaaa ttagaattgg gtgggatgaa aagcatatta atttcaaaga
     6001 actattacca aaattagaag aagcaggcgt gagtttaatt gccattcact tgcgaacaac
     6061 aaaacaactt tatggcggaa aagctcgcta cgatttagcg caaaatattc aaaatttagt
     6121 aagtataccg ctcgttgtaa gcggggatat ttatacactt gatgatgcga taaaggcgct
     6181 tgaaataact ggcgcacaag cagtgatgct agcgcgcggt caacttggaa atccgttttt
     6241 agcaacacag attgagacat actttaaaac gggagagaga ttaaaagatc cctcactata
     6301 ccagcaatta gattatttgt ggacactggc aaatatgatg gtcgaagaaa aaggagaaca
     6361 catgggaatc ttagtcatgc gcggaatcgc tcatcacttt attaaagggt ttccgtttac
     6421 caaaacatac aaagttaaac taactaaaat gtcgacttta aacgacttag aattaataat
     6481 taaggagatt aagaaccagg ctttgcttat ataagcaaaa agtggtaaaa tgtccacatc
     6541 taaaggagtt atttatgcca agagaattta gtgatcaaga attagtgaga cgcgctaaag
     6601 cggaagaact tagtgcgcaa ggtatcgatc catttggaaa aaagtatgaa cgaacagata
     6661 ctagtctttc aattaaagaa aaagctggtg aatttactaa agaagaatta gaagaaaaag
     6721 ctattaaagt taaaacagcg ggacgcataa tgctaatgcg tattatgggt aaagctagtt
     6781 tttttacgat ccaagaccaa gaaggaagaa ttcaagcctt tattagtaaa caaggtgttg
     6841 gtgaagaagt ttacgactta tttaaaagtg ctgatattgg tgacatcgtt ggtgttgaag
     6901 gaacaattat gcgtaccaaa acgggcgaag tcacagtgaa ggttgaagtt tatacccata
     6961 ttgtaaaagc attacgacca cttccggaaa agtttcacgg attaactgat gttgaagagc
     7021 gttatcgtcg ccgttatgtt gatttaataa tgaatgaatc atcaaaacat gttgccttaa
     7081 ctcgtccgaa aattattcgt gcgattcaaa aattcttaga tgatcgtgga tttgttgaag
     7141 ttgaaacaag tatgttaaat agtgtacttg gtggcgcgag cgctaaacca tttattactc
     7201 atcataatac gcttaatcgt gacttttacc tgcgaattgc cacggaatta ccattaaaac
     7261 gcttattagt cggtggcatt gaaaaagtat atgaaattgg tcgtctcttc cgtaacgaag
     7321 ggatgagcac gcgtcataat ccagaattta caacggttga agcttatcaa gcttacgctg
     7381 atttagatga tatgtatgaa ctttgcgaaa gcctagtaag agaacttgcg attaaagtca
     7441 caggtgatac taaattacca tggggcgatg aaattattga tgttggtgcg ccgttccgca
     7501 aagtatcaat ggttgatttg gtcaaagaaa agacaggtgt tgactttaga aaaattaaaa
     7561 catttgaaga agcaaaagca attgcggata aacatgaaat tcatgttgaa aagcactgga
     7621 ctggctcggg tcatattctt aatgccttct ttgaagaatt ttgtgaagca gacttaattc
     7681 aaccaacatt tgtatatggg catccacttg aaatttcccc actagcgaaa aaagccgaag
     7741 atccgcgttt tacagaacgc tttgaattat ttattaaagc aaacgaacta gcgaacgcct
     7801 ttactgaact taatgatccg cgcgatcaat atgaacgctt taaagcacaa ttaaaagcgc
     7861 gagaactagg tgatgaagaa gctagtgaaa tggacgaaga cttcgttgaa gcattagaat
     7921 acgggatgcc accggcaggc gggattggaa tcggtattga tcgtcttgta atgtttttat
     7981 taaatcaaac atcaattcgg gaagttattc ttttcccaac aatgcgtgat aataaataaa
     8041 ataaaattgt aattattact agcgaacaag gtgtgccttt acaaacgcta gtaatagtaa
     8101 tataattaca acgctatatt actgcttgta atatactcca tgctgttggt aagcaacagc
     8161 cgaaatagac caaagggagg tgtgcttatg aaaaagtacg aaatcatgta tattcttctt
     8221 gcagacttaa gcgaagaaga acgtaaagcc gaaatggaaa gtctccatca aatgattacc
     8281 aatttaggcg gtaaaattgt tgatgtagat accgaaaatt ggggcttacg tgattttgcg
     8341 tatccgatta atgatttact taaaggatat tatgtagtca ttaatgtcga aagtgatgct
     8401 gcggcaatcg ctgaattcga acggttatca aaattaaata aaaatgtaat tcgtcatctt
     8461 gttatcgcta aataaaaacg aggaggaata acaatgatta atcgagtagt cttagtggga
     8521 agacttaccc acgacccaga attacgtaaa acatcgtcag gtgcatccgt taccaacttc
     8581 agcatcgcag taaacaatcg tttcgctcgc gaccaagaaa atagtacaag tttctttaga
     8641 gtctcatgct ggaaccaaac cgcagacttt gtcgctcaat acgcacgcaa aggaaactta
     8701 gtgggagttg atggacgcat tgaacaacgt tcttatgtaa ataatgctgg acaaaaagtt
     8761 aataccgtag acattattgc cgattcggtt caattacttg agtccaaatc tgaaagtagt
     8821 gaaggaaccg gttatcaaag tgatgctgaa cccgctactg acttaggaga aggaatcgac
     8881 atctctgatg atgatcttcc attctaaatt attaaaggag aatagtttat tatgtcatat
     8941 cgcaaaagta gaccacgacg taaagtttgc tatttcaaac aaaacaaaat tactgaaata
     9001 gattataaaa atgttgagct tttaaaacgt tttgttactc caaacggaaa aattactccg
     9061 cgtagagtta ctggaacatc tgcaaagtat caacgtcaac ttgcagtcgc aattaaaaac
     9121 gcacgcttta tgggattact tccatattac gtgagctaaa aaaagaatga agagagagcc
     9181 tcacgacagt gaggttcttt attttagtta attatgaaga aaacaaaatt ttattatttt
     9241 gaattaggct tagtaatatt aattacatta gggctttatg ttttttccac taatttttta
     9301 ttactacatt atattggatt tctaggtcca gtaccgcttg cggtttttat tattcgtcat
     9361 aaaattaaag acggaatatt tcccgccatt tttatgatat taatgggaac attaattgga
     9421 cactttttac ctgtgggcca aggtagtttt atgcgtggtt tcttatttat gatttacgca
     9481 atgacagtag gatttttaca cggcacgatt agcaaaacga aaattaatcc cttgatcgaa
     9541 atattaatta tcattggcgt agaaatattt tatggctttt tattagtcat cgttttctat
     9601 gttcaaaaag atccgcactt tgcttatgat atccaatttg gtcagtatct agaattattt
     9661 attaaactat ttaagataga tgaaacatca aattacgcgc aaaatgtcgg tgttattatt
     9721 aaaaatggtg ttattgctta cacaattgta tttagtttta taagtgtttt attaacgcat
     9781 attctcattc acttaacacg aaaatttatc tacagagaaa aaatggaaca cggcccatta
     9841 cacggtttag agtttactat ttcgcgtgtc gcggcaataa cttatttagt tggttgtgta
     9901 gtagttttag tactatcttt ccttttaaca aaggaattaa atctgctagg aatttctagt
     9961 atcaacgcga tctttactgt tttcttttct gtcacaatta tttttgtgtt ccaaggtatt
    10021 ttagcggtta atcttcgcct tgaggctaag ggttgcaata atcttagtac gctagtattc
    10081 atcagtggta tattactttc tcccatcttt gcaataattg gagcgattaa tccgctctta
    10141 ccccgcaaag aaaaagcggt tgtataatca ttaaaataaa atcgctaata aacaaattgt
    10201 ttattcataa tcaaatttat gctaaattgt atatatagga attaaattat ggccagaaag
    10261 aaaaagaatg atgttgcttt atatgatgac tcttcatacg aagaagatga tttaaagtac
    10321 gctgctgatg aagaagttta tgctgaatca ttagaagatg atgaaggcaa agcaaaaaag
    10381 aaacgcaaga aaaagaaaaa agaaccaaaa gaccccaaag agcggaagga cggaagcgaa
    10441 acgcgtggcg gtaaaacttt aacagaccgc tatcattaca gaggtcgata ttatctttta
    10501 cctgaacgcg aattacgtaa acaactgcgc ttctatcaaa aagtatttaa ttatcggttt
    10561 ttaggaatca ttgcaggtgt gattgcctta atcttttcta ttctttcgct gacatttaat
    10621 gcttattttg cagtctttac actacttatt gttatcacgg gactaatcta tggtctttta
    10681 ggaatgctta agttacgtgg cctaaattgg ctttttgtct taattggaat tggtttaaat
    10741 atcatggcga ttgtattagc gatgaaacca ttaacatttg tgtttaaaca tcttggtgaa
    10801 attatggcgc tcattaatga atacatgagt tctgccgggt cttaattaca ctagttatca
    10861 acattaaagt aataattttc aacaaaatat atattatggg acatgaaagg tattaaagat
    10921 acctttttta ttgtataatt actctaaaga aaaacgaggt attgctatga tcaaagctat
    10981 tactaaatta cgcaaacgaa cgattatcct tgttatagtt gaaggacttt tactaggttt
    11041 aattagtctc ttctattatt taaacttatt taattttcaa gaaatctttg taccgccagt
    11101 tattttttct gtcgcgatag gtgtcgtttt ctttaatttg ttcgttttta ttttcattac
    11161 cgcacgaata aataaattac ggaaaaagaa tacatttgaa cttgtaaact tattaggtgg
    11221 cgatgttaaa gaggcttaca actttggaat gctaggtctt gttgtcgtcg ataatgattt
    11281 taatgtcaca tgggccagtg aattatttaa agaacgtcgt cttttaatta ttgataaaaa
    11341 cattcttgct tggaagcctg aattaattcg gcttgttgaa ctaagtgaag tgcttgcaga
    11401 taatgttgaa gacacaatta aagttgaaat cgaatcacgg aactacgaag ttaaatattt
    11461 aagcaaggct ggtattttct tgtttaaaga tattactgaa tttgaatatc tctttgatag
    11521 taatcaagca acagcacctg ttttaggcgt agtggttatt gataactttg tggatattgt
    11581 tgttaaaggt gaacactcta atccaatttt tgggacagtt aacagtttaa ttctcgaata
    11641 cttttcaaat tatggggtgc tgcttaaaca atatcgtgaa gatgcttatc tacttatttt
    11701 aaacaaagag aactttgata agttatacgc tgataatttt tcaattttac aaaaaatcaa
    11761 agataaaaca tcgaatcaag attatcaaat ttcgttatca atggcgtttg cgtataattt
    11821 ccctgatgtt ttaaaactaa atgaactcgt ccttaatgcg attgatacag caatggcgcg
    11881 tggtggtgac caagttgtta ttgcccgtta cggtgaagaa agtgaattta tcggtggcgc
    11941 aacttcacaa gctgaaaaac gtaataaaac agcgattagg ttatatgcgg atacaatttt
    12001 ccggcatata agagaagcaa aaaatgtttt aatcatgaca cacaccgata ctgactttga
    12061 cgctttgggt gcgtcgcttg gtttaaaagc tatggccgac tatgtaaaga aaacacaaaa
    12121 aggcacaaag gttcaatttc aacctgcacg catagtttat gaacacaatt taactgaatt
    12181 aaaaacaaga atggctgtaa acagaatgtt tacgaaacaa gaaatgaatg atatgtttat
    12241 ttcaccagaa gaaattgacg atcaaaaagg aaccgcgagt ctagttaatt ccggcacact
    12301 tttaattatt gctgatgttt catgtccaca attaacgctt catccaaaac tccttgaacg
    12361 cgtggataag gtaattgtga ttgaccatca tcgtcgcaca gacgaattta ttgatcaaag
    12421 actttatgac tatattgaaa caagcgcaag ttcaacttgt gaaattgtga ctgaactact
    12481 acattacggt aatactccta acttcgtatt accaccaaaa tatgcgacct ttatgcttgc
    12541 tggaatttat ttagacacaa actactttag aacaaaaaca acaggatcac gtactttctt
    12601 agcaagtatg gtgttagaag attggggcgc tgatgttatg acagcggacg atttcttaaa
    12661 ggataatgtt gaagaatatg cacttattac caaaattatg aatagcatga caacacttag
    12721 tgttggtatt gtcttcttaa gagcagatga aacagacatt gttaccccag aaactttatc
    12781 gaaagttgca aaccaaagca tgcgcattaa aggtatacaa gcttcgtttg ttattggtta
    12841 cacggcgcct aaggtcgtta aagtcagcgg tagaagtaac ggtgaaatta atgttcaatt
    12901 cttacttgag aaagttggag gcggtggtca ccaagcaagc gctgcagctg tttttacgga
    12961 taagacatta gatgatgtcc aagaaatttt agaagatgta attaatcaaa atctaagtca
    13021 agccaaagtc gttggcggca agaagggagg ataaaatgaa agtaatttta ttaaaagacg
    13081 ttaaaaaggt cggaaaaaag gatgaaattg ttgatgttag tgatggctac gccacaaact
    13141 ttttaatccc acgcaaactt gctgttccgt ataatgaacg gactatttcc caattaaaag
    13201 caaatcaagc gcgtgcttta gccgaagaaa aagcacataa agaagaagcg cttgtcaaca
    13261 agaaaaaact agaagaaaaa gaaattagtt ttattctcgc tattggtgaa aaaggtaaag
    13321 cttttggggc gattaccgct aaaaaagttg aagaaaagat taaagaagaa tttaaaatta
    13381 atgttgatcg ccgtaagttt gtgaactttc agccactaaa tcaattagga cccacaatag
    13441 tggaaattga actttttaaa gatgtcattg ctaaagtaaa agttctagtc aaagataagg
    13501 ag
//
LOCUS       DAOSLP010000027        13326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_4724_length_13326_cov_7.729184, whole genome shotgun sequence.
ACCESSION   DAOSLP010000027 DAOSLP010000000
VERSION     DAOSLP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13326
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_13326_cov_7.729184"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1622
                     /gene="hisS"
                     /locus_tag="PK460_03430"
     CDS             60..1622
                     /gene="hisS"
                     /locus_tag="PK460_03430"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOE54167.1"
                     /translation="MAIITSPKGTHDLIGNEDRAYAYISDTCGIVARLYGFDPLTTPI
                     FEANELFARGVGDATDIVRKEMYVFTDKGNRLMALRPEGTAGVMRSIIANKLYATNDL
                     PLRYYYSGPMFRYERPQLGRYRQFHQFGVESVGSTSIIADLEVILLAIHSLQALGLEN
                     ITTKINSLGKQEARDAYREALKKYYEPHLKDVCPDCKVRYEQNPLRMLDCKVPSDVEL
                     AKAAPKVTDFLTEEDKKDLEFIKTSLEEYGVNVEVDYGLVRGLDYYSGIIFEFVYIPP
                     SGKDYGAIGGGGRYDKLVEELGGPSLEGVGFAFGLERLYHILNEEGKLEHTAVKDDFI
                     FVAIGEEARTGGYSLLTALRANGLRGIMNFEDKSFKSLFKIAENRGARVALILGEAEI
                     KNEVVSVRNLETQEQVEVPFAEVINYILDLCEEYHACNCGCDHEDCDCDNHDHKCKCG
                     CEHEDCDCDHEECECDSHKENGECCGEHHHDEKHECCRKHEHEKNHECTGKGKGRGVN
                     CKKHEEKEEEKA"
     gene            1627..3366
                     /gene="aspS"
                     /locus_tag="PK460_03435"
     CDS             1627..3366
                     /gene="aspS"
                     /locus_tag="PK460_03435"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOE54168.1"
                     /translation="MYRTIENGNIIEKLVGQTVKIIGWVAKKRDLGGLTFVDLRDRSG
                     IVQVLINEGVARPDIRNEYIIQIEGVVTLKEVPNKNLKTGKVEVVASKVTLINKAKNP
                     PLIIADETDALEDVRLKYRYLDLRRKVLQDKLIARAKITKVTREFLDNEGFIEIETPI
                     LTKSTPGGARDYLIPSRLNPGTFYALAQSPQIYKQLLMIGGLEKYYQIARCFRDEDLR
                     SDRQPDFTQIDIETSFFTYEEVLDLNERLLQKIFKEVKGYDLKLPLPRLTYHEAVENY
                     GSDKPDLRFDLKIRDLSKVLSNEEYFKAKEDVRGLIINDPTNILSRKTLDELNLIVKQ
                     HGLNFLGVLKYDGTAFSGSLAKNITSSHTEVAEALGLAANDVVLLGFDAPLARILSAL
                     GAVRKELGVRFNLIDENAYSCLWVDSFPLFSYDPETRRFASEHHPFTQPKEEDIAKLT
                     TAPEEVLSYAYDIVINGYEAGGGSMRIYDQDLQNRIFEILGLSEKEIEYLFGFFIEAL
                     KYGTPPHGGIAFGLDRLTMILTGTDDIRDVIAFPKNLKGASLMSGEPSKVSNLQLDEL
                     YIKINTGKDGGEE"
     gene            3370..5616
                     /gene="tkt"
                     /locus_tag="PK460_03440"
     CDS             3370..5616
                     /gene="tkt"
                     /locus_tag="PK460_03440"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOE54169.1"
                     /translation="MCLRKRNTYDALSVAAIRATVIDITNTANSGHPGMAIGSAPLVY
                     NLYKNHLKIDPRNPEWIARDRFVLSAGHASALLYTVLHLAGYAIEIDDLKAFRQLNSI
                     TPGHPEYKMTPGVDATTGPLGQGLANAVGMALAEASLTARNPKYAHLLSHYTYCLVGD
                     GCLQEGISQEAVSFAGHQKLNKLIVFYDANDVTLDGPLSDSFSENVEARFKASGWNVL
                     KVDNGNNLKQIDKAIKKAKRSDKPTLVIMKTIIGQGSRQQGTHKVHGSPLGELDGTFA
                     KGTYHYPNAPFEIPLPVYESMRSQIARNGQKAYDTWAKSYTLFEADSDVEAEDLKIAL
                     NDDTESSLAMMKLTFEDELSEATRNTSGNVLAAVNRHLPHIMGGSADVASSVMTLING
                     ETNFTAKNRRGRNINFGIREFAMGAISNGMLLHGGLRTYVGTFLVFSDYLKNSMRLAA
                     LSHLPNIYLFSHDSVNLGEDGPTHQPVEHLAMMRAMPNFDLWRPGDARETFVAWKVAL
                     ESKTRPHALILTRQKLPLMAGSDKEKLIRRGAYVVSPENKRKKLDFIIIATGSEVNMA
                     LKAQNTLLIHGYNVRVVSMPSMEVFRRQDDAYKAEVLPLGKDKRIAVEILSSFGWHEW
                     ADHTMCLDRFGFSAPQKDILEHLKYTPEMLARLVEGIIDPKTLKKGEELYSGEAPNPI
                     PPVLSDLDTPAEGEELLTLEDVELKVSEKVEPEVTASETKEKQEATEAKAEVKTEAKP
                     KTKKTTKK"
     gene            5833..6156
                     /locus_tag="PK460_03445"
     CDS             5833..6156
                     /locus_tag="PK460_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOE54170.1"
                     /translation="MAEYVYIQKYNKQGIMGISYQVFDKIAEIATNEVSGATVSRKRY
                     SLSKPVRCTIRSGTVNVKISVKVSPNTNAEEVANEVRENVALSLTQMTELIPFDIDVE
                     VIDVG"
     gene            6168..6650
                     /locus_tag="PK460_03450"
     CDS             6168..6650
                     /locus_tag="PK460_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54171.1"
                     /translation="MKEKSRINPKKIVFKLINFFLLLIIFSNLIGNFFGISSDYTYYH
                     WGLNSIAIFSRYIENSVLEIGMRIFLLGLTTMVSSALLFLLYHFARKGKIVPIVLAII
                     IYVIDYSLVFSPWYRESTFSYIWSSFIHLIMIALFMAAGLFLLLLNPGGNKEYYDFTE
                     "
     gene            6634..7044
                     /locus_tag="PK460_03455"
     CDS             6634..7044
                     /locus_tag="PK460_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOE54172.1"
                     /translation="MISRNKSHEYGVQSVYAMLVFIKEDIAFDLPAVVSGVTGTEYSE
                     LPVFLKETLINIVKNYSEIVTNLTPYLRDWKFERLNLVTQAILIYSYAVYFYTEDKGK
                     SKAVIINVAIKLSKLFNPTEDYKFINAVLDGVLI"
     gene            7044..8300
                     /gene="xseA"
                     /locus_tag="PK460_03460"
     CDS             7044..8300
                     /gene="xseA"
                     /locus_tag="PK460_03460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOE54173.1"
                     /translation="MRSILSISELNAYVKNVIQRDAFLSYVEVRGEIINLKRYNTAIY
                     FTLKEEDKARLSVVSFNTKAFPTTLKDGDEVIATGRITVYEKSGNYQLVATEVSYYGL
                     GAKLLELAKLKVKLDKMGLFDQEKKRPLPKFPKVIGIITPKDSAAQSDLVTNINRRYP
                     LVTLKIVNATFQGESAPSSLISAFEVLQNEHIDVLIIARGGGSSDDLWAFNDESLIMA
                     LSAKKVPLISAIGHEIDTTLVDYLADVRASTPTAAAELVVPDQNELRQTLTDYSVRLE
                     NAMSTVLIQREKELNHLIIRPVLVNFGAIVENLTTKLLTLSTKLGARSAENIARAEII
                     LHSLKERLNKAITRTYNVINETLVAKSTRLEALNPTNVLNRGYAFISKNEEIIKSAKK
                     LKVKDEVKITFSDGSVLAEVKGGEDE"
     gene            8293..8484
                     /gene="xseB"
                     /locus_tag="PK460_03465"
     CDS             8293..8484
                     /gene="xseB"
                     /locus_tag="PK460_03465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOE54174.1"
                     /translation="MSKEKLSFEQKLARLNAIVSELESGKLSLDASLKLFEEGNALSK
                     ELATELNEAKLKIEELQGK"
     gene            8643..9812
                     /locus_tag="PK460_03470"
     CDS             8643..9812
                     /locus_tag="PK460_03470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOE54175.1"
                     /translation="MSKVILHIDLNAFFAACEVLRDPTLKGKPLIVGGANRRGIVSTA
                     SYEARKYGIHSAMPTYKALRLCPNLIIKEPDFKLYHHYSSLFFNYLKDHVSPLLEVAS
                     IDECYIDATERLKNNRNPIGFIKELQQTLLQEIGLGCSIGVAPTKFLAKMASNYKKPL
                     GITIYRRRELANTLWKLPINEMYGIGKATAPRLLALDIKTIGDLATTEDPEVKKLLGK
                     SFFTFKEWANGRGSDEVIAEEEDPKSIGHSSTFMFDTENYEEISALFKEMSNSVSERA
                     KREGKVGLTIQIVMRYYNFENVNRSVTLNKPTNNAITIFYEAMKLFDKNYKDEPLRLV
                     GVTLQNLSDEGSLVEQLSLFDDFNEKSKTEVIIELLNEEYDKPPFLRLSDLKRDD"
     gene            9815..10687
                     /locus_tag="PK460_03475"
     CDS             9815..10687
                     /locus_tag="PK460_03475"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOE54176.1"
                     /translation="MHIDEAVRRYTNFLLLEKNLANTTVSAYLDDINLFHTTFNFTVT
                     EEISPMHVSDFINLESQKGLRASTIMRRLSSIYNYLLFLKDEGDYKYELKRIPGPAVT
                     KTLPTYLTNDEVDRLLSMPNLNTHRGVRDKAMLEVMYASGLRVSELLSLKREEVNFNR
                     GIIKIRGKGGKERIIPIGEFALEYLADYINNVRFHYEKSDKRTIFLNTNGKPLSRQYF
                     FKMIKAYAQEAEITANVSPHTLRHAFATHLLENGADLRAVQAMLGHTNIATTQIYTHV
                     STKRIISAYDKIMK"
     gene            11204..11986
                     /locus_tag="PK460_03480"
     CDS             11204..11986
                     /locus_tag="PK460_03480"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOE54177.1"
                     /translation="MKRRGFLISLITFMMISLTSCFKLAVQHKIHKFGYYEYIITGVH
                     KRLPRGGPAVVRIIGLTQSGLQQSVLDIPRTINDLPVVALGYGPMKSGGFPSPPYTFS
                     SKNLRKLYIHDNLVNIERGAFSTPENQNEFVPFNIMICAYRFEQIGEFNLNQLNKRFI
                     YRYIFENRDIDSNIKWGNSAIPANVEFLNNYSDEVNGGYYRLDNIEDGELIPIPPDPF
                     REGYIFDGWYSESECTNKFDFDIAPTIAENTELKIYAKWISA"
     gene            12046..13254
                     /locus_tag="PK460_03485"
     CDS             12046..13254
                     /locus_tag="PK460_03485"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOE54178.1"
                     /translation="MKNKKFKRVFVVVMDSVGVGAMPDAHLYGDAGSHTLKHTAESKK
                     NGLHVPNLEKYGLGNLDDIKGVKKVKNHPNSYVMKMLEASTGKDTMTGHWEFMGIHTE
                     KPFKTFTDTGFPQALIDELSAKTGYKIIGNYSASGTEIIKELGEEQMRDNSLIVYTSA
                     DSVLQIAAHEEVTGLDELYRCCEIAREICLRPEYLLGRIIARPYIGSDPSNFKRTANR
                     HDYALSPPEKTALNILDDNGITVSAIGKINDIYNGFGITQAQKTISNEDGMDKTIAQA
                     KANEFNGLIFTNLVEFDSEYGHRRNPVGYADAIERFDKRLGELMEVVTEDDLVLLTAD
                     HGNDPTWHGTDHTREMVPLIAISKAFTKGRVLEHRKSFADIGATILANYNLKLTPTLI
                     GEVIDELLTE"
BASE COUNT         4445 a         2241 c         2729 g         3911 t
ORIGIN      
        1 gaaaggagat gtattaccta atcgaagtac cggggcatga ttagataata caaggatata
       61 tggctattat tacgagccct aagggcacac atgacttaat tggaaacgaa gaccgtgctt
      121 atgcttatat aagtgataca tgcggcatcg ttgcccgact ttatggtttt gatccactga
      181 caacaccgat tttcgaggct aatgaactat ttgcccgtgg tgtcggtgat gcgacagaca
      241 ttgtacggaa agaaatgtat gtatttaccg ataaaggtaa ccgcttaatg gcactgcgtc
      301 ctgaaggtac cgcaggagta atgcgtagca ttattgctaa taagctttac gctacgaatg
      361 atttaccgct gcgttattac tacagtggac caatgttccg ctatgaaaga ccacagcttg
      421 ggcgttatcg acaattccat caatttgggg ttgaaagtgt tggtagtacg agcattattg
      481 ctgatcttga agtaatttta cttgcaatcc attctttaca agctttagga cttgaaaata
      541 ttacaactaa aattaatagt cttgggaagc aagaagcgcg tgacgcctat cgcgaagcac
      601 ttaaaaaata ttatgaacca catttaaagg atgtatgtcc tgattgtaaa gtgcgttatg
      661 aacaaaatcc gctacggatg cttgattgta aagtaccaag tgacgtcgaa cttgccaaag
      721 cggcaccaaa agttactgat ttcttaactg aagaagataa aaaagacctt gaattcatta
      781 aaacttcact ggaagaatat ggtgttaatg tggaagttga ttacggatta gtgcgcggac
      841 ttgattatta ttcaggcatt atctttgaat ttgtctatat cccgccttca ggtaaagatt
      901 acggagcgat tggtggcggt ggccgctatg ataaacttgt ggaagagtta ggcggtccaa
      961 gtttagaagg tgttggtttt gcttttggcc ttgaacggct ctatcacatt ttaaatgaag
     1021 aaggaaaact tgagcacacc gcagttaaag atgactttat ttttgtcgcg atcggtgaag
     1081 aagcacggac tggtggttat agtttactaa ctgctttacg cgcgaacggg ctacgcggaa
     1141 tcatgaattt tgaagataaa tcatttaaat cactctttaa aatcgccgaa aatcgtggag
     1201 cacgtgttgc tttaattctc ggcgaagctg aaattaaaaa tgaagttgtc agtgtacgga
     1261 acttagaaac acaagagcaa gttgaagtac cgtttgccga agtgattaat tatatacttg
     1321 acctttgtga agaatatcac gcctgtaatt gtgggtgtga ccatgaagat tgcgattgtg
     1381 ataatcatga tcataaatgt aaatgcggct gtgaacatga ggattgcgac tgtgaccatg
     1441 aagagtgtga atgcgactct cataaagaaa acggtgaatg ctgcggcgaa catcatcacg
     1501 atgaaaagca tgaatgctgc cgtaaacatg aacatgaaaa aaatcatgaa tgcactggca
     1561 aaggtaaagg tcgtggtgtg aactgcaaaa aacatgaaga aaaagaagaa gagaaggcat
     1621 aaacttatgt atcgaacaat tgaaaacgga aatattattg agaaactagt tggacaaaca
     1681 gttaaaatca ttggttgggt cgctaaaaaa cgtgatttag gcggcttaac atttgtggat
     1741 ttacgtgacc gaagcgggat tgtccaagtg ttaataaacg aaggcgtagc gcgccctgat
     1801 attcgcaatg aatacattat tcaaatcgaa ggcgttgtca cgcttaaaga agtgccaaat
     1861 aaaaacttaa agacagggaa agttgaagtc gtggcaagta aagtcacact tattaataaa
     1921 gctaagaacc cgccgcttat tattgccgat gagacagacg ctttagaaga tgtgcggcta
     1981 aaatatcgtt atttagattt acggcgtaaa gtattacaag ataaacttat cgcccgcgct
     2041 aaaataacga aagtaacgcg tgaattttta gataatgaag ggtttatcga aattgaaaca
     2101 ccaattttaa ctaaaagtac acctggtggt gctcgtgact atttaatccc ctcacgctta
     2161 aacccaggca ctttctacgc cctagcgcag tcaccacaaa tttataaaca acttttaatg
     2221 attggtgggc tagaaaagta ttatcaaatc gcccgctgct ttagagacga agacttacgt
     2281 agtgaccgcc aacctgattt tacccaaatt gatattgaga caagtttctt tacttacgaa
     2341 gaagtacttg atttaaatga aagactttta caaaaaatct ttaaagaagt gaaaggctat
     2401 gatttaaaat tgcccttacc acgcttaact tatcacgaag ctgtagaaaa ttatggtagt
     2461 gataaacctg atttacgttt tgatttaaaa attagagatt taagtaaagt tttaagtaat
     2521 gaagaatatt ttaaagcaaa agaagatgtt cgtggtctca ttattaatga cccgacaaac
     2581 atcttatcgc gtaaaacact tgatgaactt aacttaatcg ttaagcaaca cgggcttaat
     2641 ttcttaggtg ttttaaagta tgacggcacc gcttttagcg gtagtcttgc aaaaaacatt
     2701 actagcagcc acactgaagt agcagaggct ttaggtttag cggcaaatga tgtcgtctta
     2761 cttgggtttg acgccccgct cgcgcgtatt ttaagtgcac taggcgcagt tagaaaagaa
     2821 ttaggcgttc gctttaactt aattgatgaa aatgcttact cctgtttatg ggtcgattcc
     2881 ttcccacttt ttagctatga tcctgaaact cgtcgcttcg ctagtgagca ccatcccttt
     2941 acgcaaccaa aagaagaaga tattgctaaa ttaacgacag caccagaaga agttttaagt
     3001 tatgcttatg acatcgttat taatgggtat gaagctgggg gcggaagtat gcggatttat
     3061 gaccaagatt tacaaaatcg tatctttgaa atcttaggct taagtgaaaa agaaattgag
     3121 tatttattcg gtttctttat cgaagcacta aaatacggca ccccaccgca cggcgggatt
     3181 gcttttgggt tagatagact gacgatgatt ttaacgggta ctgatgatat tcgtgatgta
     3241 attgccttcc ccaaaaattt aaagggcgca agtttaatga gcggtgaacc ttcaaaagtt
     3301 tctaatttac aattagatga attatatatt aaaataaata caggaaaaga tggaggagag
     3361 gaataattta tgtgtttaag aaaacgtaat acttacgatg ccttaagtgt cgcggcgatt
     3421 cgtgcgactg ttattgacat tactaatacc gctaattcgg gacaccctgg gatggcgatt
     3481 gggagtgccc cattagttta taatctttat aaaaatcatt tgaaaattga tccgcggaat
     3541 ccagaatgga ttgcccgtga ccgttttgtg cttagtgcgg gacatgcgag tgccttactt
     3601 tatactgtgc tccatttagc aggttacgcc attgaaattg atgacttaaa agcctttaga
     3661 caacttaact cgattacccc aggccaccct gaatataaaa tgacaccagg agtcgatgcc
     3721 acaacaggac cactcggtca aggacttgcg aatgccgtgg ggatggcgct agcggaagct
     3781 agtttaacag cgcgtaatcc aaagtatgcg catcttttaa gccattatac gtactgttta
     3841 gtgggcgatg gctgtttaca agaaggaatt agtcaagaag cagtctcgtt tgctggtcat
     3901 caaaaactta ataaattaat tgtgttttat gacgctaacg atgtgacgct tgacggacca
     3961 ctgagcgatt catttagtga aaatgttgaa gctcgcttta aagcaagtgg ctggaatgtt
     4021 ttaaaagttg ataacggtaa taatcttaaa caaattgata aagcaattaa aaaagccaaa
     4081 cgcagtgata aaccaacact tgtaattatg aaaacgatta tcggtcaagg tagtcggcaa
     4141 caaggtacac ataaagtcca cggctcacca cttggtgaat tagatggtac ttttgctaaa
     4201 ggtacttatc actatccaaa tgcgccattt gaaatcccgc ttccagtata tgaaagtatg
     4261 cgaagccaaa tcgcgcgtaa cggccaaaag gcgtatgaca cctgggcaaa gagttataca
     4321 ttatttgaag ccgacagtga tgttgaagcc gaagacttaa aaatcgcctt aaacgatgat
     4381 actgaaagta gtctagcaat gatgaaactt acatttgaag acgaattaag tgaagcaaca
     4441 cgcaacacta gtggtaatgt actcgctgct gttaatcgtc atttaccgca tattatgggc
     4501 ggaagtgctg acgttgctag tagtgtaatg acactaatta acggtgaaac aaactttacg
     4561 gctaaaaacc gtagaggacg gaatatcaat ttcgggattc gtgaattcgc gatgggagcg
     4621 attagtaacg ggatgctcct tcacggcgga ctacgcactt atgtagggac attcctcgtc
     4681 tttagtgatt accttaaaaa ctcaatgcgt cttgctgcac taagtcactt accaaatatt
     4741 tatttattta gtcatgatag tgtgaattta ggagaagacg gaccaaccca tcaaccggtt
     4801 gaacacttag cgatgatgcg cgcaatgcca aactttgact tatggcggcc gggtgacgca
     4861 cgcgaaacat ttgtggcgtg gaaagtagcc ctagagagca aaacgcggcc acacgcctta
     4921 atcttaacac ggcaaaaatt accacttatg gctggtagtg ataaagaaaa gctaatccgt
     4981 cgtggtgctt atgttgtaag ccctgaaaat aaacgaaaga aacttgattt tattattatc
     5041 gcaacgggta gtgaagtaaa tatggcctta aaagcgcaaa atactttact catccacggt
     5101 tataatgtcc gtgttgtctc gatgccgagc atggaagtgt tccgtcgtca agatgacgct
     5161 tataaagctg aagtattacc acttggcaaa gataaacgca tcgctgttga aatcttaagt
     5221 agctttgggt ggcacgaatg ggcagatcat acgatgtgct tagatcgctt cggctttagt
     5281 gccccgcaaa aagatatttt agagcactta aaatatacac cagaaatgct cgcacgcctt
     5341 gttgaaggga ttattgatcc aaagacactt aaaaaaggtg aagaactcta ttccggtgaa
     5401 gcacctaatc caatcccgcc cgttttaagt gatcttgaca ccccagcgga aggtgaagaa
     5461 cttttaacac tagaagatgt tgaacttaaa gtttcagaaa aagttgaacc tgaagtaacc
     5521 gctagtgaaa caaaagaaaa acaagaagca actgaagcaa aggccgaagt aaaaactgaa
     5581 gcaaaaccaa aaacaaagaa aacaactaaa aaataatcaa ttagtagttt attagtagtt
     5641 aattttaatt ggattactta gaatggcgca ttgcaaatat gcgccatttt ataattctca
     5701 ctatataaaa tttatttttt aaattgttaa attaacttat tcaattatca ttcaataata
     5761 aattattggt caaaagcaac aaatattgta aaaaggtgtt tgtttcgtta taattaaagc
     5821 cagaggttat ttatggcaga gtatgtttac attcaaaaat acaataagca aggcataatg
     5881 ggtattagct atcaagtatt cgataaaatt gccgagattg ctactaatga agttagtggg
     5941 gcaacggttt cacgtaaacg ttattcgctt tctaaaccag tacgttgtac gatccgtagt
     6001 ggtactgtta atgtgaagat ttccgtaaaa gttagtccga atacaaatgc tgaagaagtt
     6061 gcaaatgaag tccgtgaaaa tgtcgcgctt agtttaacgc aaatgaccga attaattccg
     6121 tttgacattg atgttgaagt aatcgatgtc ggttaagcat tatattgatg aaagagaagt
     6181 cacgaataaa tccgaaaaag atcgttttta agttaattaa ctttttttta ttattaatca
     6241 tttttagtaa cttaattggt aatttctttg gcatcagtag tgattatacg tattaccact
     6301 ggggattaaa cagtattgcg attttttcgc gttacattga aaatagtgtc ttagaaattg
     6361 ggatgcgcat ctttttatta ggcttaacga caatggtaag cagcgctctt ttatttcttt
     6421 tgtatcattt tgcgagaaaa ggtaaaattg taccgatagt gttagcaatt atcatttatg
     6481 taattgatta ctcactagtg tttagtccgt ggtaccgtga aagtaccttt agttatatat
     6541 ggtcgagctt tattcattta attatgatcg cattatttat ggctgccgga ctattcttat
     6601 tactactaaa tccaggagga aataaggaat actatgattt cacggaataa aagtcatgag
     6661 tatggtgtgc aaagtgtcta tgcaatgctc gttttcatta aagaagacat tgcttttgat
     6721 ttacctgcgg ttgtaagcgg tgtaactggt acagaatata gtgaactccc cgtatttctt
     6781 aaagaaacat taattaacat cgttaagaac tatagtgaaa ttgtgacgaa cttaacacct
     6841 tatttacgcg actggaagtt tgaacgttta aatttagtaa cccaagccat cttaatttat
     6901 agttacgctg tttattttta tactgaagat aaaggtaaaa gtaaagcagt gattattaat
     6961 gtcgctatta aattatcaaa actttttaat ccgaccgaag attataaatt tattaatgct
     7021 gtgcttgatg gggttttaat ttaatgcgaa gtattttatc aattagtgaa cttaatgcat
     7081 acgttaaaaa tgttattcag cgtgacgcat ttttaagtta tgttgaagta cgcggagaaa
     7141 ttattaactt aaaacgctat aatactgcga tttacttcac cttaaaagaa gaagataagg
     7201 cacgccttag tgttgtaagt tttaacacta aagcgtttcc tacgacgcta aaagatggtg
     7261 acgaagtaat tgccacaggt cgtattacgg tttatgaaaa aagtggtaac taccaactcg
     7321 tggccactga agttagttat tacggacttg gtgcgaagtt gcttgaactt gctaaactta
     7381 aagtaaagtt agacaagatg gggctttttg atcaagaaaa gaagcgccca ttacctaaat
     7441 ttcccaaagt tattggtatt attaccccaa aagatagcgc ggcacagagt gatttagtga
     7501 cgaatattaa ccgtcgctat ccgcttgtga ctttaaagat tgttaacgct acttttcaag
     7561 gggaaagtgc gccttcttca ctcattagtg cctttgaagt tttacaaaat gaacatattg
     7621 atgtcttaat cattgcccgt ggcggtggaa gtagtgacga cttatgggca tttaatgatg
     7681 aaagtctcat tatggcctta agtgcaaaga aagtcccact tattagtgcg ataggacatg
     7741 aaatcgatac aacattagtc gattatcttg ccgatgtgcg cgctagtacc ccaactgctg
     7801 ccgccgaact tgttgttcct gatcaaaatg aattacggca aacgcttact gattatagtg
     7861 ttcgtcttga aaacgctatg agtactgtgc tcatacaacg cgaaaaagaa ctgaatcact
     7921 taataataag accagtactt gtaaactttg gcgcaattgt ggaaaactta acgacaaagc
     7981 ttttgacact gagcactaaa ttaggagccc gtagcgccga aaacatcgct cgagcggaaa
     8041 ttatcttgca ttcattaaaa gaacgcttaa ataaggcgat aactcgtacg tataatgtta
     8101 ttaatgaaac gcttgtcgct aagagtacac gacttgaagc acttaatccg acgaatgtat
     8161 taaatagagg ctatgctttt attagtaaga atgaagaaat tattaaatca gcgaagaagt
     8221 taaaagtaaa agacgaagtt aagattacat ttagtgacgg cagtgtacta gccgaagtta
     8281 aaggaggaga agatgagtaa agaaaaatta tcatttgaac aaaaactcgc acgcttaaat
     8341 gctattgtta gtgaactaga aagcgggaaa cttagtttag acgctagttt aaaactcttt
     8401 gaagaaggca acgctttaag taaagaacta gcgacagaat taaatgaagc aaaactgaaa
     8461 attgaagaat tacaaggaaa ataacgctaa aacgaccctt tagtatgtta ttagtaatta
     8521 gttagtagtc ttttagttgc tttatagtgt tataaaatag gacattcgtt cctatttttt
     8581 attaggaact tattatttac ataattatta tgtaaaccaa aatagtgagt tataatatat
     8641 ttatgagtaa agttatctta cacattgacc ttaacgcttt ctttgccgcg tgcgaagtac
     8701 tgcgtgaccc taccctaaag ggtaaacctt taattgttgg tggggctaac aggcgcggaa
     8761 tcgttagcac ggcgagttac gaagctcgga aatacggtat tcattcggcc atgccgacat
     8821 ataaagcgct gcgcttgtgt ccaaatttaa ttattaaaga acctgatttt aagctttatc
     8881 atcattattc ttcgctcttt tttaattatt taaaagatca tgttagtccc cttcttgaag
     8941 ttgctagtat tgatgaatgc tatatcgatg cgaccgaacg gcttaaaaat aaccgtaatc
     9001 caatcggttt tattaaagag ttacaacaaa ctttactaca agaaatagga cttggctgct
     9061 cgatcggtgt tgctcccact aaatttttag caaagatggc aagtaactat aaaaaaccac
     9121 ttggtatcac tatttatcga cgccgtgaat tagcgaacac tttatggaaa ttaccaatta
     9181 atgaaatgta cgggattggt aaagcgaccg caccccgtct tttagcgctc gatattaaaa
     9241 caattggaga ccttgctaca actgaagacc cggaagtgaa aaagttgctt ggtaaaagct
     9301 tttttacctt caaagagtgg gcaaacggca gagggagtga cgaagtaatt gccgaagagg
     9361 aagatccaaa aagtattggt cactcatcaa cctttatgtt tgacactgaa aattatgaag
     9421 agataagtgc tttatttaaa gaaatgtcta atagtgttag tgaaagagca aagcgtgaag
     9481 gaaaggtcgg cttaacgatt caaattgtca tgcgctatta taattttgaa aatgtaaatc
     9541 gcagtgtcac gcttaataaa cccacaaata atgccattac gatattttat gaagcgatga
     9601 agctttttga taaaaattat aaagatgaac cgctccggct tgtaggggta acactacaaa
     9661 atcttagtga cgaagggagt cttgtcgagc aactttcatt atttgacgac tttaatgaga
     9721 aatcaaaaac agaagttatt attgaattat taaatgaaga atacgataaa ccaccgtttt
     9781 tacggctaag tgacttaaaa agggatgatt aaagatgcat attgatgaag cagtgagacg
     9841 ctacactaat ttcttattac ttgagaaaaa ccttgctaat acgacggtaa gtgcttattt
     9901 agatgatatt aatctctttc atacgacctt taacttcaca gttaccgaag aaatttcgcc
     9961 aatgcatgtt agtgacttta ttaatttaga aagtcaaaaa ggacttaggg catcaacaat
    10021 aatgcggaga ctatcaagca tttataatta tttgctcttt ttaaaagatg aaggtgatta
    10081 taaatatgaa cttaaacgca ttcctggacc agcagtcact aaaacattgc caacatatct
    10141 cactaatgat gaagttgatc gacttttaag tatgccaaac ttaaacactc atcgcggtgt
    10201 tcgcgataaa gcgatgcttg aagtaatgta tgctagcgga ttacgagtta gcgagctttt
    10261 aagtttaaag cgtgaagaag taaattttaa tcgtggcatc attaaaattc gcgggaaagg
    10321 tgggaaagag cggattatcc caattggtga attcgccctc gaatacttag cggattatat
    10381 aaataatgtt cgctttcatt atgaaaaaag tgataaacgc actattttct taaacactaa
    10441 tggtaaacca ttaagtcgtc aatatttctt taaaatgatt aaagcttatg cgcaagaagc
    10501 tgaaataacc gctaatgtat caccgcatac attacgccac gcttttgcca cacacttact
    10561 cgaaaatggc gccgatttaa gagccgtaca agcaatgctc ggccacacta atattgctac
    10621 aacgcaaata tatacacatg tgtccactaa acggatcatc agtgcatatg acaagattat
    10681 gaaataataa ggtgttaaat taataaagca caaactatta tttgttataa taaaaaagaa
    10741 atcgaaatgt actgataccc taaatgtaga cacacaataa aatcatgtgg ccacatgatt
    10801 ttgctaaaat acattcagga ggaaaacatg tacagtaaag aagagaaaag agccattggc
    10861 aaagaagcct atgacaaagt tgacacttta gcgaacctag cgcgaacact atgcagcttg
    10921 tgaatgtgga caagacggaa cgtttggtca tgtaattgat ggtaataact atctattacc
    10981 tggacgacat tatcaatgtt tagtatgtgg tggtcctgct aatttgggtt tcgttttcgg
    11041 ggcgaaaaat aatgataaca tcacggtttg tacagaaaat ggtagttatt gtctgcctaa
    11101 tggtattctt gttcttgttg gtgatgatgt agaagctttc caaaatggaa cgcttggatt
    11161 tattgaagat tattatagac attcaaaaaa ggagtttcat tagatgaaaa gaaggggatt
    11221 cttaatatca ttaattacat ttatgatgat ttcattaaca tcatgtttta aacttgcggt
    11281 tcaacacaag attcataaat ttggctacta tgaatatata attacaggag tccataagcg
    11341 tttaccaaga ggaggacctg cggtagttag aattatcggg ttaacacaaa gtggtttgca
    11401 acaaagtgtg ctagatatac cgcgtactat aaatgattta ccagtggtag cacttggcta
    11461 tggtccaatg aaatcagggg gttttcctag cccaccttat acattcagta gcaaaaatct
    11521 gcgaaaattg tatattcacg ataatcttgt aaatatcgaa cgaggtgcgt tttcgactcc
    11581 agaaaatcaa aatgaatttg taccatttaa tattatgata tgtgcttatc gttttgaaca
    11641 aatcggtgaa tttaacttaa atcaactcaa taaaagattc atatatcgat acatttttga
    11701 aaaccgtgat attgattcaa acataaaatg ggggaatagt gcgattccgg cgaatgttga
    11761 atttctcaat aattattctg atgaagtaaa tggtggttat tatcgccttg ataatattga
    11821 agatggtgaa ctcattccaa tcccacctga tccttttcga gaaggttata tatttgatgg
    11881 ttggtattcg gaaagtgaat gtaccaataa atttgatttt gacatagcgc ccacaattgc
    11941 cgaaaacact gaacttaaaa tatacgctaa atggattagt gcttaaccat atatataatg
    12001 ttttattatt tgttataata aacctgaaat cgaaaggatt aaattatgaa aaataagaag
    12061 tttaaacgcg tctttgttgt cgttatggat agtgtcggtg ttggtgctat gcctgatgca
    12121 catttatatg gtgacgctgg tagtcatacc ttaaaacata cagcggaaag taaaaagaat
    12181 ggattacatg ttcctaattt agaaaaatat ggcttaggta atcttgatga tattaaaggt
    12241 gttaaaaagg ttaaaaacca ccctaattca tatgtcatga aaatgttaga agctagcact
    12301 ggtaaagata caatgactgg ccactgggaa tttatgggga ttcatacgga aaaaccgttt
    12361 aaaacattta ccgatacagg tttcccgcaa gcactaattg atgaattaag tgcgaaaaca
    12421 ggttataaga tcattggtaa ttattcagca agtggtacgg agattattaa agaactcggt
    12481 gaagaacaaa tgcgtgataa tagcttaatt gtttatacat ccgcggatag tgttttacaa
    12541 atagcagcgc atgaagaagt aacaggactc gacgaattat atcgctgttg tgaaattgcc
    12601 cgtgaaattt gtttacggcc tgaatacctt cttggtagaa ttattgctcg tccttatatc
    12661 ggtagtgacc caagcaactt taaacggacc gctaatcggc atgattatgc actttctcca
    12721 ccggaaaaaa cggcacttaa tattcttgat gataacggca taaccgtcag tgcaatcggt
    12781 aaaattaatg atatttataa cggttttggc attacacaag cacaaaagac tataagtaat
    12841 gaagacggaa tggataaaac gattgcccaa gcgaaggcta atgaatttaa cggtttaatt
    12901 ttcactaatc tcgttgaatt tgatagtgaa tacggtcata gacgcaatcc agtaggctat
    12961 gcagatgcca ttgaacgctt tgataaacgt ctcggtgaat taatggaagt cgttactgaa
    13021 gacgatttag tgcttttaac tgctgaccac ggtaacgatc caacatggca cggcactgac
    13081 catacccgtg aaatggtacc gcttattgct attagcaaag catttactaa agggcgtgtg
    13141 cttgaacacc gtaaaagctt tgccgacatt ggtgcgacaa tcctcgctaa ttacaattta
    13201 aagttaaccc caaccttaat tggtgaagta atcgacgaat tactaaccga ataactcata
    13261 ttatcctcac tttgtactga taccctaaat gtagacacac aataaaatca tgtggccaca
    13321 tgattt
//
LOCUS       DAOSLP010000028        13155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_4786_length_13155_cov_7.356870, whole genome shotgun sequence.
ACCESSION   DAOSLP010000028 DAOSLP010000000
VERSION     DAOSLP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4786_length_13155_cov_7.356870"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..776
                     /locus_tag="PK460_03490"
     CDS             132..776
                     /locus_tag="PK460_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54179.1"
                     /translation="MKTLNEGESNTIKKPTSALEKALVILLVVFLGATITMLVFYHFA
                     RWGLGLNIFVFLLILIVPALLLLGVIIIIVNIAGKRNFEISWKRPLLPTFIVLGAGVF
                     SLLFVIGERATFKIRYTFTIEKWAKATPDERSVLVDSFLEQYDLNTFNDAMVIEYLGE
                     PDFKEILEVGEPPSFAGYYYIYNLGFVRDFIDPTFFDIKTDASGKVVYYNVRYT"
     gene            796..1836
                     /locus_tag="PK460_03495"
     CDS             796..1836
                     /locus_tag="PK460_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54180.1"
                     /translation="MKRKKQVFRASLTFIALSFLLIGSLLNNKEDYFYRLKGETTYSC
                     ALPAGYVEINTIASTALTLTSHGGGNDYHNKVNTDTFRGTVTRKAGNTAYIQRRNQSN
                     GELDSLYLWGVGTYSDLVPGAVIDISGGMIVNYYGNPEIKGFTYTVPFATNPYGYGPL
                     VYNSVSDFNINAFTNIYDNSALNYSRLVKVKNVTLNALITEGAQNRTAIRNIYNNANS
                     SGLWNFDYYIQDLAIPYLICNAEFKSGDYSFKWYKGGTDGQINFYMLVPFNPSETDTI
                     SFETSFSGAGYKYDSGNEKYANQFQFYLYKITERYDWFRIDKVTFDKTNGKFKCEVFS
                     NVDNSTTTFTEV"
     gene            1989..3227
                     /locus_tag="PK460_03500"
     CDS             1989..3227
                     /locus_tag="PK460_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE54181.1"
                     /translation="MQEIKRDLYLNKLINAIGNRMIKVVTGIRRSGKSYLLNNLFKNY
                     LLESGVSADHIIILNMDERRNISYRDPDTLDEYIRSKIKDNEMYYVIIDEVQFVADFE
                     SVLNGFLNMHNLDVYVSGSNSKFLSNDIITEFRGRGQQIRVYPLSFSEFMSVTTLSKS
                     DALTEYLRYGGMPLLMSMKSDEAKARYLKDLFELTYLKDIIERNNVLRDDVLDELVNI
                     LASAVGSLTSVTKLTNTFKSKGVKELSKNTLIQYLDYLLDSFLIEKVSRYDVKGKKYI
                     QTLAKYYFSDIGLRNARLNFRQQEENHIIENVVYNELIRRGFNVDVGIVEIRNGNGIK
                     RLEVDFVCNEASRRYYIQVTTHLDTREKTLQEQRPLLHIGDNFKKIIIVRDDIKPWYT
                     EEGILVLGLYDFLLDNDSIL"
     gene            3401..3664
                     /locus_tag="PK460_03505"
     CDS             3401..3664
                     /locus_tag="PK460_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54182.1"
                     /translation="MQTFNYIGLIVVALLLIPNLMYYLKHKEEVVSKNGIFKTLAIIG
                     ISGSLFFMVINIPYTWLQFYFMFAREMYFISNVNSLLFMYLFS"
     gene            3739..4326
                     /locus_tag="PK460_03510"
                     /pseudo
     CDS             3739..4326
                     /locus_tag="PK460_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            4413..4646
                     /locus_tag="PK460_03515"
     CDS             4413..4646
                     /locus_tag="PK460_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54183.1"
                     /translation="MSLKKEPLDTYLYREFSIAETTHKSGERKWGEGMSKNSKKGREN
                     PICIKNNYEFKYEEFKEYKGKNGRFELLVVEIQ"
     gene            5077..6399
                     /locus_tag="PK460_03520"
     CDS             5077..6399
                     /locus_tag="PK460_03520"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE54184.1"
                     /translation="MKREIMQDLISWKNKRNRKPLLLTGVRQCGKTYILRKFGEEHFD
                     NYVYLNLERDPQIAAVFNYDYDTKRILQDISNLSLLPEIIPNKTLLIIDEIQFNPKAI
                     TALKYFAEDMPELCIVGAGSLLGVSLREEGASFPVGKVDRLEMYPMSFKEFLLESKSR
                     RIVNSIENFDITKLLPDYILDNLNREYTNYLLVGGMPEAVKVWFENHNLAEVNEIQDN
                     IIAGYENDFSKHSPKNELTNIELIWRSIPEQLAEDNNKFVFSRVKKSARAKDLEKSIG
                     WLIDAGLIRVVTKVDNPQVPLSFYSNSSFYKIYLCDVGLLSRMAGFTLKSLLEKDEST
                     GGFRGSLSENFINNELVSLGYVTYFWRSKNSAEVDFLIEDDGRVIPIEVKANVNTKAK
                     SYNNFIKKYATKIGFRFSLKNSGENKVGVTKTYSLPLSLVFRLKDYLN"
     gene            6563..7063
                     /locus_tag="PK460_03525"
     CDS             6563..7063
                     /locus_tag="PK460_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOE54185.1"
                     /translation="MSKITHVKNFVGIYSIVFAVLGFVALIIDKTHEPIIHILLTLFF
                     ILGVIVHVPFLLKIHLVSMIVMGFLHGSLLIMSIIVMFHDCLTGLFGFGLFILPLVVS
                     IVYFVMYSNDKKKQKNLLVAALDSNNTKESNTLNDELNALLASKNRGEITEEEYLAKK
                     KDLIGY"
     gene            complement(7060..7782)
                     /locus_tag="PK460_03530"
     CDS             complement(7060..7782)
                     /locus_tag="PK460_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54186.1"
                     /translation="MKKAKPIKFKQKRKRWYRALKRIIKVRYKEPKFIFLGEKPTTNA
                     IILSNHVGTDAPMSLEIYAPFHLRMWGTHEMNSGLRKMYRYQSRVYYHEKKGWNLHLA
                     RLFCLLASPLTNLFYKGLRLISTYQDLRLKKTVKESLEAFKDGDNIVIFPEVSDEGYL
                     DELKGFHGGFCILAEIAYKHGIDTYLYASYFKIKEKVYLFDKPILYSELKKIAPTRLE
                     MARYLVDKCNALGKLDLNKIKS"
     gene            8052..8642
                     /locus_tag="PK460_03535"
     CDS             8052..8642
                     /locus_tag="PK460_03535"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOE54187.1"
                     /translation="MFQNFDGGYIEVVCGPMFAGKTEELIRRIKRLEYAKQSYIIFKP
                     KIDGRYGDNVIQSHNNNRREAVNIEKAREIYTHLKDKTVEVIIIDEAQFFDEELPDVV
                     DDLADQGYRVIVGGLDRNFRGEPFGTMPEILALAEVVTKLTAICPVSGKPATRTQRII
                     DGKPAHYNDPLVLIDAEESYEPRSRAQHIVPGKKKK"
     gene            complement(8677..9033)
                     /locus_tag="PK460_03540"
     CDS             complement(8677..9033)
                     /locus_tag="PK460_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54188.1"
                     /translation="MKHITIVYDEKLRSFGIKVIKVRPGAFKTDMQINVTHKFEKVFA
                     YTKDFKSPLTKMQKMMLTELERAKDPRKITKTFLKAIYKKRPKTVYKVNNSFKMKLLN
                     ALSVKVQDFILGLFFK"
     gene            complement(9100..9483)
                     /locus_tag="PK460_03545"
     CDS             complement(9100..9483)
                     /locus_tag="PK460_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE54189.1"
                     /translation="MKYALITGASGGLAHNIITAIKDDFFLILTDINPAISDKYNDLP
                     NKLIVVSDLTDKDSINNLFDKILKITDKLDLVIHFAGILEIGPIMEVKVEALETLMQV
                     NLFAVYFINQKAFPLLKKLKVALFI"
     gene            9603..10280
                     /locus_tag="PK460_03550"
     CDS             9603..10280
                     /locus_tag="PK460_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOE54190.1"
                     /translation="MNVFVYIFFGLFIAVTIAELIMAFNEIEKARMILKPFSMFFLAI
                     AALIAVPNHPLIYLGAFLGMVGDIFLLWPKNKKMFLAGATSFLVGHTLYISEIIHIMF
                     KGQALPWWFYAVVSFGVLLFVLAFYPMSKKISGDRYLTLVGNTYIAVLILVSVVSIIA
                     CVKGFTNFMVLGIIGGISFLASDLILVQATFVKDFKRRDYYIMLFYLLGQALIVSGLV
                     LTYVFAA"
     gene            10315..10533
                     /locus_tag="PK460_03555"
     CDS             10315..10533
                     /locus_tag="PK460_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54191.1"
                     /translation="MKKHNLLFITLTVVTLLSGCKKYRSRGSVLSGELLRNNITKVLK
                     KAEPLNVKKIYEQLYLIMMKFITIMTLH"
     gene            10593..10925
                     /locus_tag="PK460_03560"
     CDS             10593..10925
                     /locus_tag="PK460_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54192.1"
                     /translation="MRKQIIHNIFKKALVRDIYVTNNEVSGAFIRVYYDELYIFDGFG
                     HLLYQGTQEIHSISSGINNEGKHYVKLYINPVFTTPITYDEVYFYVYDALGNISKVAT
                     DPAPNTQK"
     gene            10944..11162
                     /locus_tag="PK460_03565"
     CDS             10944..11162
                     /locus_tag="PK460_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54193.1"
                     /translation="MSEYGLPLYSLVTNEESQEIKDATGKTIVTLKKEVGVYRSQIFA
                     GHYFYQKITKGSKSAINYDLKDIYGNTY"
     gene            11163..11927
                     /locus_tag="PK460_03570"
     CDS             11163..11927
                     /locus_tag="PK460_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54194.1"
                     /translation="MSTHSVNLKTGRIKTLKFPYFLAAISPLYDKNNIYNYAVAEIAT
                     FDAFNSKTANYVSEKFIINYKGHLLDQVSDMPLENFVEIKGGFYNKATNKLYDEDVKL
                     IKEFKDFTSSIVLAQQGLIIGTKDGKDGVINARGDEVIPFIYDKIFDSEKEGYMIANK
                     SNMSYRIKLATNEATSLSSVVTKAGNEKTPLYTYFDASRLYPITVLNEKEIVYQFRIE
                     GEVSTYWQPTFMTFTQSAMPGYNVVLKAQVIIDTKI"
     gene            12153..13028
                     /locus_tag="PK460_03575"
     CDS             12153..13028
                     /locus_tag="PK460_03575"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HOE54195.1"
                     /translation="MKKCVFYTALSGLALTIFGLIVAFFGYRLDLFVEAIPKLVALFP
                     TLWWAILPPAVGLLVLIINIAVKAKKKNKCAIVPFALFVSILAYLLVVPRALPEWLAE
                     GVAPESLVFTTGAAVVVIVFLVLFVLAFVDMALPTRKQLRAEKELDEEVYEVVEEKPT
                     RGPHDAPVLYSDEEVEDEEGLEEVSEAERKVAEKPKAKKAKAKETEKAKEEEEEVKKE
                     DVKKEKEYDRIYHIMKRAKDDRWIVKIAQSRKAIKIFDTQKEAIAYAEILASNNNGVV
                     RVFASKGANKGRIIQ"
BASE COUNT         4456 a         1840 c         2441 g         4418 t
ORIGIN      
        1 taagtaaaat tcataaacga aagaaacaat taacttgttc attgaatttt atgttaagtt
       61 aattaaaaag tctttgttat gaaaatatta ttagcaaaag tgcaaattta ttaatttgct
      121 ataatttgat tatgaaaact ttgaatgaag gagaaagtaa cacgataaaa aagcccacta
      181 gtgcacttga aaaagcctta gttattttac ttgtggtttt tttaggcgcg actataacaa
      241 tgctcgtctt ttatcacttt gcgcgctggg ggttagggct taatattttt gtatttttat
      301 taatattaat tgtaccagcg cttttactgc ttggtgtcat cattattatc gtcaatattg
      361 ctggaaaaag aaattttgaa atatcgtgga aaagaccact attgccaaca tttattgtac
      421 ttggtgcggg agtattttca cttttattcg tgattggaga acgggctact tttaaaatcc
      481 ggtacacttt tacgattgag aagtgggcaa aagcaacacc cgatgaacgt agtgttctag
      541 ttgattcttt tcttgaacaa tacgatttaa atacatttaa tgatgcaatg gttattgaat
      601 atttaggtga gcctgacttt aaagaaatac ttgaagttgg tgaaccccct tcatttgcgg
      661 gttattatta tatttataat cttggttttg tgcgtgattt tattgatccg acattttttg
      721 atattaaaac tgacgctagt ggtaaagttg tttattacaa tgttagatat acgtaaatta
      781 ggagaagtac tatttatgaa acgaaaaaag caagtgttta gagcttcctt aacttttatt
      841 gctctttctt ttttgcttat tgggtcatta cttaataata aagaagatta tttttataga
      901 cttaaaggtg aaacaactta ttcatgtgct ctccctgcgg gatatgttga aattaacact
      961 attgcgagca ctgctttaac gcttactagt cacggtggcg gtaatgatta tcataataaa
     1021 gtaaacactg atacttttcg aggtacagtt acgcgtaaag ctgggaatac ggcctacatt
     1081 cagcggcgta atcaaagtaa tggagaatta gatagtcttt acttatgggg tgtgggaact
     1141 tacagtgact tagtacccgg tgccgtcatt gatataagcg ggggcatgat tgttaattat
     1201 tacggtaatc ctgaaattaa aggttttact tatacagtgc cttttgcgac taatccctat
     1261 ggctacggac cactagtgta taacagtgtt agtgatttta acataaatgc tttcacgaat
     1321 atttatgaca attctgcact taattatagc cgcttagtaa aggtcaaaaa tgtgacgctt
     1381 aatgcgctta ttactgaagg ggcacaaaac cgcacggcga ttagaaatat ttacaataac
     1441 gctaattcat cagggttatg gaattttgat tattatatac aagatttagc gataccctat
     1501 cttatttgta acgcggaatt taaaagcgga gattacagct ttaaatggta taaaggcggc
     1561 actgatgggc aaattaattt ctatatgctc gttcctttta atccgagtga gactgataca
     1621 atttcctttg agacttcctt tagtggcgcg ggctataagt atgatagtgg caatgaaaaa
     1681 tacgcaaatc aattccaatt ttatctctat aaaattaccg agcgttacga ttggtttaga
     1741 attgataaag taacctttga taaaacaaac ggtaaattta aatgtgaagt attttcaaac
     1801 gtcgataatt cgacaacaac atttaccgaa gtttaatagt aaaacattta cttataataa
     1861 tattcgcact gaacaaaaac tacaaaaatg taagttttgt tcaatgactc ttgatattca
     1921 caaacaaata ttttaaaata gtactgaaca aaaactacaa aagcgtaagt tttgttcagt
     1981 gaggtgttat gcaagagata aaacgtgatt tatatttgaa taagttaatt aatgcaattg
     2041 gcaaccgcat gattaaagta gtgactggta ttcggcgctc aggcaaatca tatttgttaa
     2101 ataatctttt caaaaattat ttattagaaa gtggtgttag tgcagaccac atcattattt
     2161 taaatatgga tgaaaggcga aatatttctt atcgtgaccc cgatacttta gacgaataca
     2221 ttcgcagcaa aattaaagat aatgaaatgt attatgtcat cattgatgaa gtgcagtttg
     2281 ttgccgattt tgaatcagtg cttaatggat ttcttaatat gcataactta gatgtttatg
     2341 tttctggtag taattcaaaa tttctatcaa atgatataat cactgaattt aggggtagag
     2401 gacaacaaat tcgcgtgtat ccactttctt ttagtgaatt tatgagtgtc accactttaa
     2461 gtaaaagtga tgctttaact gaatatttac gctatggcgg gatgccgctt ttaatgtcaa
     2521 tgaaaagtga tgaagcaaag gcgcgttatc ttaaagattt atttgagctt acttatctca
     2581 aagacattat cgagcggaat aatgtgctgc gtgatgatgt tttggatgag cttgtaaata
     2641 tattggcctc tgctgttggt tctttaacta gtgttactaa attaacgaat acctttaaga
     2701 gtaaaggtgt caaggaacta tcgaaaaata cgcttattca gtatttagat tacctattag
     2761 attcttttct tatcgagaaa gttagtcgtt atgatgttaa aggtaaaaaa tatattcaaa
     2821 ctttagcaaa gtattatttt agtgatattg gattacgtaa tgccaggctt aatttccggc
     2881 aacaagaaga aaatcacatt attgaaaatg ttgtctataa cgaacttata agacgtggtt
     2941 ttaatgttga tgttggtatc gttgaaatta gaaatggaaa tggtattaag cgccttgaag
     3001 ttgattttgt ttgtaacgag gcgagtagac gctattacat tcaagtaacg acgcatcttg
     3061 atactagaga aaaaacattg caagaacaaa gaccactgct acatattggc gataacttta
     3121 agaagattat tattgtccgc gatgatatta agccttggta tacagaagaa ggtattttag
     3181 tgctaggact ttatgatttc ttacttgata atgatagtat tctctaaaca cttattgtag
     3241 gctgttcttt aattggattg tgcttaatat aataaaaaat gaaaggtttt ttaattgtca
     3301 tttcttacat attctttaca aacaagcagc aataaaagta ttgaacttat gttaaaatta
     3361 tgtggaataa agacaaaggg taatgttagg agtaatagtt atgcaaacat ttaattacat
     3421 tggtttaatt gtggtagcgt tacttttaat acctaatttg atgtactatc taaaacacaa
     3481 agaagaagtt gtttctaaaa atggtatctt taaaacttta gcgataattg gtatcagtgg
     3541 atcactattt tttatggtga ttaatattcc ttatacttgg ctacaatttt attttatgtt
     3601 tgcgcgagaa atgtacttta ttagtaacgt aaactcgcta ttatttatgt atttattttc
     3661 ttagtaactt gggataaaaa gagtgtagta cgcgcgatat tattaacgat tattcctttt
     3721 tgtatattta ctttttcagc gataatgctt ggttgtttag atgaaaaaat taccgctgtt
     3781 aacccggatt caaaattatc tttatttggt caagaatata atcctgaaac ttatgcaata
     3841 gcaaaagctg acatgttaat taaaggtggt aatgcccaaa acatgaaatt tggcaatact
     3901 ttaaatgacg accaatttag tggttatgaa tttgattata ttatttcaaa tccgccattt
     3961 gggattgatt ggaaaagtga agcaaaggat gttaagaaag aacatgaaaa attaggtttc
     4021 agtggtagat ttggccccgg tttaccagca ataagtgaca gtcaaatgtt atttatgtta
     4081 aatggtgtca aaaagttaaa agaaggaagc ggaagaatgg ctatcattca aaacggctct
     4141 tcacttttta ctggtgatgc tggtagtggg ccaagcgaaa ttagaagata tattattgaa
     4201 aacgatatgt tagaagcgat tattcagtta ccaacagatt tattttataa tacttcgatt
     4261 acaacctata tatggttaat tacaaaagga aaatcaagtg aacgtattgg taaagttcac
     4321 ttgattgttg gaaagatatt tcaagaattg aaacatttgt aacatcagat aatacgatta
     4381 gttatggata ttttatttta ataactaatg atttgagttt aaagaaggaa ccattagaca
     4441 cttatttata tagagaattt agtattgctg aaaccaccca caaaagtggg gaaagaaaat
     4501 ggggtgaagg tatgtctaag aactctaaaa aaggtcgaga aaatccaatt tgtattaaaa
     4561 ataattatga atttaaatat gaagaattta aggaatataa aggaaaaaat ggcagattcg
     4621 aattacttgt cgttgaaatt caatagcagt tgtatttaat tatctaataa tgatgtaata
     4681 aaatattaag tgatatcgct aatattaata gtattgctct aaatataatc gaaatgaata
     4741 aaattgctga gtaatataat aaataaataa ataattatta atgtgcatct ttaacaatga
     4801 atattaattt atagttttat gcattttatg acaataatca aaaagcgtca ccactggtga
     4861 cacaattaga tttgcctaac aattgactaa aattataatg tgacttttaa cacatgaata
     4921 aaggtgatga agcgcaaaaa tcatacgaat aattctcaat attttgcaaa atgcatagga
     4981 ataatttaaa aataattgca aaaaccatag gaataattta aaaataattg caaaaaggat
     5041 aggaatgttt taaaattgag gcaggagatg tactttatga aacgggaaat tatgcaagat
     5101 cttatttctt ggaaaaataa gagaaataga aaaccactct tattaactgg agttaggcag
     5161 tgcggaaaaa cttatatttt aagaaaattt ggtgaagagc atttcgataa ctatgtctat
     5221 ttaaatcttg aaagagatcc acaaattgct gccgtcttta attatgatta tgatacaaag
     5281 agaattttac aagatataag caatttaagt ttattacctg aaatcatccc taataaaacg
     5341 ctcttaatta ttgatgaaat tcaatttaat cctaaagcga taaccgcact taaatatttt
     5401 gcggaagata tgcctgaatt atgcatagtg ggagcgggtt ctctactcgg ggtatcgctt
     5461 agagaagaag gggcgtcctt tcctgtaggt aaagtggacc gtttagaaat gtatccaatg
     5521 agttttaaag aattcttgct tgaatcgaaa agtaggagaa tagtaaatag tatcgagaac
     5581 ttcgatataa caaaactctt gccagattac attttggaca atttgaatag ggagtatacc
     5641 aattatcttt tagtgggtgg tatgcctgaa gctgttaaag tgtggtttga aaatcataat
     5701 ttagcggaag ttaatgaaat acaagacaat ataatcgcag gttatgaaaa cgatttctcg
     5761 aagcacagtc caaagaatga attaactaat atagaactta tttggcgatc tatccctgag
     5821 cagcttgcag aagataacaa taaatttgta ttttcaagag taaaaaaatc agcgagagct
     5881 aaagatttgg aaaaatcaat cggttggctt atcgatgctg gacttattcg tgtagttact
     5941 aaagtcgata atccgcaagt tcctttatcg ttttatagta attcctcgtt ttacaagatt
     6001 tatttgtgcg atgtcggact cttatcgaga atggctggtt ttacgcttaa atcgttactc
     6061 gaaaaagatg agtcaacagg tggatttagg ggctctttga gtgaaaactt tataaataat
     6121 gagctagttt cattaggata tgtgacttac ttttggcgat caaaaaatag tgcagaagtc
     6181 gactttctaa ttgaggatga tgggagagtt attccaatag aagtaaaggc aaatgtcaat
     6241 acaaaggcta aaagttacaa taattttata aaaaaatatg caaccaaaat tggttttaga
     6301 ttttcactaa aaaatagcgg tgaaaataaa gttggagtca caaaaactta tagtttgccg
     6361 ttatctttag tatttagatt aaaagactat ctgaactaat ataatttccc gcggaaaaac
     6421 tacaactact ggaataaaat tccatcaatc actggaatac agtagtgatt acaagtttaa
     6481 aagtgaggtt attcttaact ctttagtgca taaaatatct ttttaatcta taattaaaag
     6541 aaatagaagg ggtagtagtt ttatgagtaa aattacacat gtcaaaaact ttgtgggtat
     6601 ctattcaatt gtttttgctg tattaggctt tgtggcttta atcatcgata aaacccatga
     6661 accgattatt catattttat taacactctt ttttatcctt ggtgttattg tccatgtccc
     6721 gtttttatta aagattcatt tagtgtcaat gattgtgatg gggtttctac atggttcatt
     6781 acttatcatg agtatcattg ttatgtttca tgactgttta acgggtttat ttggttttgg
     6841 tttatttatt cttcctttag tggttagcat tgtttatttt gttatgtaca gtaacgataa
     6901 gaaaaagcaa aagaatttat tagtagctgc attagatagt aataacacaa aagaatctaa
     6961 cactttaaat gatgaattaa atgcgctttt agcatctaaa aacaggggcg aaataaccga
     7021 agaagaatat cttgcaaaaa agaaagattt aattggttat taagatttaa ttttattcaa
     7081 atctaattta cctaacgcat tacatttatc aactaaatag cgtgccatct ctaagcgggt
     7141 gggcgctatt tttttaagtt cactatatag aattggttta tcaaatagat aaactttttc
     7201 ttttatttta aagtatgatg catataagta tgtatcaata ccgtgcttat aagcaatttc
     7261 tgctagaatg caaaaaccac catgaaatcc ttttaattca tctaaatatc cttcgtcact
     7321 aacttcgggg aaaataacga tattgtcacc atctttaaaa gcttctaagc tttctttaac
     7381 tgtcttttta agtcgtaagt cttgataagt tgaaattagt cgtagtcctt tatagaagag
     7441 gttagttaaa ggacttgcta aaagacaaaa aagtcttgct aaatgtaagt tccagccctt
     7501 tttctcatga taatacactc gtgactggta acgatacatt ttacgcagcc ctgaattcat
     7561 ttcatgtgtc ccccacatcc gtaagtggaa tggagcgtaa atttcaagtg acatcggcgc
     7621 atcagtgccg acatgattac ttaagataat tgcatttgtc gttggttttt caccaagaaa
     7681 gataaacttt ggttctttat aacggacttt gatgatacgt tttaaagcgc ggtaccagcg
     7741 cttacgtttt tgtttaaatt taataggttt agcttttttc ataagtaccc ccacaagaaa
     7801 atattaacat atattcaata caatattatt tttttatatg tttttgacat tatctgtcaa
     7861 tattcctcat gtaagtgttt tcattttcag tgtcattttt tacaattttg aaattatgct
     7921 aatgacacta aattaatttt aaaagttgtt aaattatgtc tatcttttta atagttaagc
     7981 gtttaaaaag ttgtgttaaa tttgttaaga ttattaatga aaatatttga atagagaaaa
     8041 ggggtaagat tatgtttcaa aattttgatg gtggctatat tgaagtcgta tgtggcccga
     8101 tgtttgccgg taaaaccgaa gaattaatta gaagaattaa acgcttagaa tatgcgaaac
     8161 aatcatatat tatttttaaa ccaaagattg atggtcgcta tggcgataat gtgattcaaa
     8221 gtcacaataa taatcgtcgt gaagcagtta atattgaaaa agcacgcgaa atttatacgc
     8281 atttaaaaga taaaacagtc gaagttatta tcattgacga agcacaattt tttgatgaag
     8341 aactgcctga tgttgttgat gacctcgctg atcaaggcta ccgtgtgatt gtcggtgggt
     8401 tagaccggaa ctttagaggt gaaccatttg gcacgatgcc ggagattctt gcccttgctg
     8461 aagtcgtcac taaactaacg gcgatatgcc cggtaagcgg aaaaccagcg acgcgcaccc
     8521 agcggataat agacggtaaa cctgcgcatt acaatgaccc acttgtgtta attgatgcgg
     8581 aagaatctta tgaaccgcgt agcagggcac aacatattgt gccgggcaaa aagaaaaagt
     8641 agcgaggtgc tacttttttc ttaaatagta ttttaattat ttaaaaaata gtcctaaaat
     8701 aaaatcttga actttaacgg aaagagcatt taataatttc atcttaaacg aattatttac
     8761 tttataaaca gtttttggtc ttttcttata tattgctttt aaaaatgttt tagtgatttt
     8821 acgggggtct ttagctcttt ctaactcggt aagcatcatc ttttgcattt ttgtaagcgg
     8881 gcttttaaag tctttagtgt aggcaaatac tttttcaaat ttatgcgtaa cattaatttg
     8941 catatctgtt ttaaaagcac cgggtctaac tttaataacc ttgatgccaa aacttcgtaa
     9001 tttctcgtcg taaactatcg ttatatgctt caatcctgcg ttttgctatc gtgtagaagg
     9061 agtggaaggg tagtgctaaa agtgtgccgt actcactact taaatgaata atgcgacctt
     9121 tagctttttt aagagcggga aggctttttg gttaatgaag tagaccgcaa aaagattaac
     9181 ttgcatcaag gtttcaagtg cttcaacctt tacttccatt attggaccaa tctctaaaat
     9241 accggcgaag tgaatgacta aatcaagctt gtctgtaatt ttaagtatct tatcaaaaag
     9301 attatttatt gagtctttat ctgttaagtc gctaaccaca ataagtttat ttggtaaatc
     9361 attatactta tcacttattg ctggattaat atcagtgagt attaaaaaga agtcatcttt
     9421 gatggcagtg ataatgttgt gcgctaagcc accactagcg ccagtaataa gcgcatattt
     9481 cataaattta ttttaacact ctaaatgtaa atgagtgtca atatttggaa aatagcgcta
     9541 aattcactac tttttaataa tattaaaaga tgataatata aaagggtagg agaaatatag
     9601 ttatgaacgt ttttgtgtat atcttttttg gtttattcat tgcggtgaca attgctgaac
     9661 taattatggc ttttaatgag attgaaaaag cgcggatgat tcttaaacct ttttcaatgt
     9721 tctttttagc aattgcggca cttattgctg tacctaatca cccgcttatt tatcttggag
     9781 catttttagg gatggttggt gatatcttct tactgtggcc taaaaataag aagatgtttc
     9841 ttgcaggggc aacaagcttt ttagttggac atacattata tattagtgaa atcattcaca
     9901 ttatgtttaa aggtcaagct ttaccgtggt ggttttacgc tgtggtgagt tttggcgtgt
     9961 tgctctttgt gttagcgttt tatccaatgt ccaaaaagat tagtggtgac cggtatttaa
    10021 cacttgttgg taatacatat attgctgttt taattttagt ttctgttgtg agcattattg
    10081 cttgtgttaa ggggtttact aactttatgg tactaggtat catcggtggg attagcttct
    10141 tagcgagtga tttaatctta gtgcaggcaa cttttgtcaa agattttaaa cgccgtgatt
    10201 attacattat gcttttctat ttacttgggc aagcattaat tgtttctgga ttagtgctca
    10261 cttatgtctt tgcggcctaa tttctaatat cgatattggt aaaggaggaa cttagtgaaa
    10321 aaacataatt tactttttat tactttaacc gttgtgactt tactttcagg atgtaagaag
    10381 tataggtcgc gtggtagtgt gttaagcggg gagttactgc gtaacaacat tacaaaagtg
    10441 ctaaaaaaag cagaaccgtt aaatgttaaa aaaatatatg aacaattgta tttaatcatg
    10501 atgaaattta tcaccattat gacactgcac taattagaga tataattgcg acgaaaaatg
    10561 gtgatgattg tattggtttt tactcattag cgatgcgtaa acaaatcatt cataatatat
    10621 ttaaaaaagc cttagtgcgt gatatttatg ttactaataa tgaagtatca ggcgccttta
    10681 ttcgtgtgta ttacgacgaa ctttatattt ttgatggctt tggtcattta ctctatcaag
    10741 gcacacaaga gattcatagt ataagttcag ggataaataa tgaaggaaaa cattatgtaa
    10801 aactatatat taatcccgta ttcactactc caattactta tgacgaagtt tatttttatg
    10861 tgtatgacgc ccttggtaat atatcaaaag ttgcgactga tccagcgcca aatacacaaa
    10921 aataaagtag taacgcggaa gatttgtcag agtacggatt acctctttac tcgctagtta
    10981 caaatgaaga gagtcaagaa attaaggacg ctactggtaa aacgatagtg acgctaaaaa
    11041 aagaagtagg cgtttatcgt agccaaatct ttgcgggtca ttatttctat caaaagatca
    11101 caaaaggaag caaaagtgcg attaattacg atttaaaaga tatttatggc aacacttatt
    11161 aagtgtcaac acatagtgtt aatttaaaaa caggtcgcat taaaacgctt aagttcccct
    11221 actttttagc cgcgataagt ccactctatg ataaaaataa catttataat tatgctgttg
    11281 ctgagattgc tacttttgat gcttttaata gcaaaacagc aaattatgtt tccgagaaat
    11341 ttattattaa ttataaaggt cacttgcttg atcaagtaag tgatatgccg cttgaaaatt
    11401 ttgtggaaat taagggcgga ttttataata aagctacgaa taaactttat gatgaagacg
    11461 ttaaattaat taaagaattt aaagatttta caagtagtat agttctagca caacaaggac
    11521 ttattatcgg tacaaaagac ggtaaagatg gagtgattaa tgcgcgtggt gacgaagtaa
    11581 ttccatttat ctacgacaaa atttttgata gtgaaaaaga aggttacatg attgctaata
    11641 aaagtaatat gtcgtatcgc ataaagttag ccactaatga agccactagt ttaagcagtg
    11701 tagtcactaa agcaggtaat gaaaagacac cgctttatac atattttgat gcaagtcgcc
    11761 tctacccgat tactgtatta aatgaaaaag aaatagtgta tcaatttaga attgaaggtg
    11821 aagttagtac ttactggcaa ccgactttta tgacatttac acaaagtgca atgccgggat
    11881 ataatgttgt gctaaaagcc caagttataa ttgatactaa aatttaatat tattttgttc
    11941 actttgaata tgtagcgcac gcctataaaa atatggatac ttatggcagt gaagttttac
    12001 aaccttaaaa ggaaagtaaa gaataaagcg cttactactt taaataaaag caaaatgggg
    12061 caaatattcg gtattttagc ccctttttgt ttacttctga gtattatttt tatataatac
    12121 tattgagttt tatattagga aaaggagaac ttatgaagaa atgtgttttt tataccgctc
    12181 ttagtggtct cgccttaaca atctttggtc tcatcgtggc cttttttggt tatcggcttg
    12241 atctctttgt agaggcaatt cccaaattag tcgctttatt cccgacatta tggtgggcaa
    12301 ttttaccgcc agccgtggga cttttggtgt taattattaa catcgctgtt aaagccaaga
    12361 agaaaaataa atgtgcaatc gttccgtttg cgctatttgt gtctattctt gcttacttac
    12421 tcgtagtgcc acgcgcatta ccagaatggt tagcagaagg cgtagcacca gaaagtttag
    12481 tgtttacgac tggtgcagca gttgtcgtca ttgtattttt agtgctcttt gtcttagcgt
    12541 tcgttgatat ggcactccca acacggaaac aacttcgcgc tgaaaaagaa cttgatgaag
    12601 aagtttatga agtagttgaa gaaaagccaa cacgcggacc acatgacgct ccagtactat
    12661 acagtgatga agaagtcgaa gacgaagaag gcttagaaga agttagcgaa gctgaacgca
    12721 aagtcgcaga aaaaccaaaa gcgaaaaaag caaaagcaaa agaaacagaa aaagcaaagg
    12781 aagaggaaga agaagtgaaa aaagaagacg taaagaaaga aaaagaatac gaccgcattt
    12841 atcacatcat gaaacgggcg aaagatgatc gttggattgt taaaattgcc caaagccgca
    12901 aagcaattaa aatctttgat acacaaaaag aagcgattgc atatgctgaa attttagcga
    12961 gtaacaacaa cggagttgtt cgtgtcttcg cctcaaaagg cgccaataaa gggcgcatta
    13021 ttcagtagta aaaagaaggc acttaacaca aggtgccttt ttatatattg tgctaagtag
    13081 aaaggaatat taaagcatgg ccaaaatcat aattatttca ggtgacctcg ccgctggtaa
    13141 aagtgtgtta gcaaa
//
LOCUS       DAOSLP010000029        12874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_4919_length_12874_cov_8.025587, whole genome shotgun sequence.
ACCESSION   DAOSLP010000029 DAOSLP010000000
VERSION     DAOSLP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4919_length_12874_cov_8.025587"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1118)
                     /locus_tag="PK460_03580"
     CDS             complement(18..1118)
                     /locus_tag="PK460_03580"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOE54196.1"
                     /translation="MTEIEMKNKDRKFRLTNKTFKFPSAIKDHFYTANYFVKTMEIAE
                     KHLADTVVTMQFFQRRDNVMACGVDESLALLQEFARYPEKLKIEACHDGDIVNANEPV
                     LKITGLYKDFAYLESLIDGILTRRTTAATNSYEVLQAAYPTEVFSMADRQDDYLTQAG
                     DGYASYIAGIKKFSTDAQGAWVNVKGMGTMPHALIAMTNGDLIKACELYHKTFPNEKI
                     SALVDYHNDTVTEALKVARHFGAKLGSIRLDNSKALLDEYFVRQGLNDPSLKGVSPEL
                     VFAVREALDKEGFNYVNIVVSSGFTPEKIKYFKAKNAPVNLYGVGSFIVNNKEVGFTG
                     DVVVVNGKPQAKVGRQEYFSDKLEKVTLKKRM"
     gene            complement(1123..2193)
                     /locus_tag="PK460_03585"
     CDS             complement(1123..2193)
                     /locus_tag="PK460_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOE54197.1"
                     /translation="MLTKRELKLLEKVTQIDAIPGLEQELAKFVKGELEKYGFTIVTD
                     NLGSVFGVKKSKVTQAPRIMVAGHLDEVGFSVREITDKGFIRVDARGGINANTMLASR
                     VRLTLDDGTKLMGSVGATAPHYGGEGNTTIDKLDFDFGFISKEDAVNAGVRVGQMIVA
                     DGPFEVLNGGKRLLAKAFDNRYGVFMALEMARYFENVDLPYDLYVGATVQEEVGLRGA
                     TTIANVINPDLVIILDCSPARDNISSSEQGQLGEGILIRYLDRSMIAFPELLTFQEKS
                     AEALKVPYQYFHSPGGTDAGAIHLHAVGVLTLTHCVCARSIHTSASVVDTTDIKNAKK
                     VLIRMLRTLNRDKINKFKEARR"
     gene            complement(2202..2822)
                     /gene="trmB"
                     /locus_tag="PK460_03590"
     CDS             complement(2202..2822)
                     /gene="trmB"
                     /locus_tag="PK460_03590"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOE54198.1"
                     /translation="MRVKFKPGAIDYLKSLAFHTHVDDFALSKIKDINKPLVLEIGVG
                     KGDYLLEMATNYPEHFFIGIELNASVLSVAAQKLADANLNNILILTGDVKYLLPKLSA
                     LSLNYIILNHSDPWPKKRHEKRRLTYSTLVNEYYKLLEEGGTLIFKTDNDDFADYSYI
                     SLSSVPFKELIFIKDYKGDYDFDAKTEYERKFLNKGVKIKMIIGKK"
     gene            complement(2819..4759)
                     /locus_tag="PK460_03595"
     CDS             complement(2819..4759)
                     /locus_tag="PK460_03595"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HOE54199.1"
                     /translation="MKNKKLVSDIAIDGLFIALILVLSLVPYLGFIQIGGISATILPI
                     PVILGAALLGPRRGVLYGAAFGFSSFLIAVIRGTAGDALFVDPLISIVPRILFGFCTA
                     IFSAVSFNERTSFKLKRFLIFPYSAIMMLLHSFFVLLAMYLRYVNAFMEYIFPILTPL
                     VLLEALVATVIVPVLYNVLYIPFEKYKDKFTTKNKSIYGTITSVYFADALNSLKEFVS
                     IKSVYDEKTVTKKTPYGKGVNEALEYMKNLATNDGFEAKIIDGRVVEIFVGEKYNKNI
                     AVFAHADVVPATGEWDTPPFTADIREGKLYGRGTSDDKGPAIAAYYAIKTLNNNNLLI
                     NYSVRLVIGGDEERGSSCMHYYFNEYNAPAPVHGFTPDAEFPLIYGEKGITNFTATKM
                     IDLGPISTITGGEAANSVIDKVVIRLLKDEDFIKYLTDNKVEHTVKMLSKNMDVTIFG
                     KSAHGSLPELGVNAGVLAFKHLGAFYKLPFLTHLAEKFKNPNGKTMDAYVATSLLGAT
                     TYNIGLLNYENGKLSFVVNFRYPENVEVETHLAKLAQTIDVELEIGRSSKHLLFDPKS
                     EFIQTLLKAYRDETGDTQSKPLAIGGGTYAKECPNTVAFGSAFPSRSGDIHSANEHIY
                     LDDFYTQMAIYARAIHYLGKKV"
     gene            complement(4749..5531)
                     /locus_tag="PK460_03600"
     CDS             complement(4749..5531)
                     /locus_tag="PK460_03600"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOE54200.1"
                     /translation="MYLTVDIGNSVISIALFNAGEIVDVFNRETPKALKETAQANFDT
                     VRPIINEFKKQYAHSTFKKALISSVVPKLNYPMSFVIKEILGVDAEIVLPNPKTSVRL
                     DVDVPESVGSDIVACAVGAYKFGKGPFIVIDLGTANKYFYVDDKHVFHGVAISPGVEV
                     SFKGLVKNAAQLFNVRLTLPAKVLGKNTPDSLNSGLLYGLLGEMNGFINLIKKEVGDN
                     PIVLLTGGNARYIKGAVDESVNYVPHLVHYGLYDMLVNGNEK"
     gene            complement(5589..7988)
                     /gene="leuS"
                     /locus_tag="PK460_03605"
     CDS             complement(5589..7988)
                     /gene="leuS"
                     /locus_tag="PK460_03605"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOE54201.1"
                     /translation="MAYDFRAAEKKWQKYWEENNTFYTDMYDFSKPKYYVLDMFPYPS
                     GDGLHVGHVEGYTATDIVARFKRMQGYNVLHPIGFDAFGLPAEQYAIRTNNHPAIFTN
                     ENIANFRRQLKSLGFSYDFSKELSTSEPKYYKWTQWIFTELFKSDLAYVDNIPVNFCP
                     ELGTVLANEEVIDGKSEVGGFPVIRKPMRQWLLRITEYAERLLSDLDGLDWPASTLDM
                     QRNWIGKSEGTTITFKVANTNYEFDVFTTRVDTLYGATYACLAPEHPLVLKVVSDTQK
                     EEVKRYIESASHKSDLERTELNKDKSGVYLGVNAINPLNNKEIPLYVADYVLGSYGEG
                     AVMAVPAHDERDYEFAKRHGLPMVQVLAGDISENAFIDDAPHINSPLINGLNIEESKK
                     VLNEHLTNKGVGRVTTNYKLRDWLFSRQRYWGEPIPIMIKEDNTFIPVPLSDLPLTLP
                     PLKEYKPSGTGESPLALATSWVNTTYEGEKVKRETNTMPQWAGSCWYYIRYLDPHNDE
                     KIADPELIKHWLPVDLYIGGAEHAVLHLLYARFWHKFLYDKGVVNTKEPFTKLFHQGM
                     ILGENGEKMSKSRGNVVKPDDIIAEYSVDALRLYEMFMGPLEASLPWSNTGLEGALRF
                     LNRVARINSEDFPGKLVDTNNGELDYSYHYTVKKVTEDYEKLAFNTAIAQMMILVNDM
                     YKAREVYIPYYVGLIKLLYPIAPHLGEELYSQFVDVDTITYEAWPTYDEAKLVVNEVE
                     IAVQVNGKLRATIKAKLNESKEELEKAALTNENVQRFIDGLTIRRVIVVPNKIVNIVA
                     S"
     gene            complement(8163..9596)
                     /locus_tag="PK460_03610"
     CDS             complement(8163..9596)
                     /locus_tag="PK460_03610"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOE54202.1"
                     /translation="MKILMVASESSPLAKTGGLADVVYALSKELAILGEEVSVVMPFY
                     GNIKRSPDQEIRFVANVQVPLGWRFQNAKVYKTFIDGITFYLLENEYYFGREGFYGYD
                     DDTERFAFFSVAVRNMMQALKIKPDVVHVHDYHGGMVPALIKIQNSRTKFFNKMKFVV
                     TIHNPAFQGEFHPDLLPDFYDIRKEYFDDGTLRYRDKVNALKAAIVFADKINTVSPTH
                     AEELLTPEGSKGLDGILKLRKNDFSGILNGIDYDEFNPQTDKHLPFRYGKTRPGIKTK
                     NKLVLFKELGIKGDVDTPLFSMVSRLTWQKGIDLVLPAIVEVLEKGGNVVVVGSGEHG
                     YEQALEELRARYPDRMAIYIGYNNPLAHRVYAASDFFFMPSLFEPCGIGQMIAQRYGT
                     LPIVRTTGGLKDTVIGYDGTNEDVADGFVFDNYDEYWMKLTTNYALEQYKNQKVFKKL
                     RRNAMEKNNDWSEAGKKYLALYKSIVK"
     gene            complement(9596..10711)
                     /gene="glgD"
                     /locus_tag="PK460_03615"
     CDS             complement(9596..10711)
                     /gene="glgD"
                     /locus_tag="PK460_03615"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOE54203.1"
                     /translation="MKVLAYLNLHHAPRLGPLTTRRSTAVVSFLGRYAIMDFMLSNFS
                     NSGFDRIATLVDNHPDSVLKHFGSRNTFNINTKLGLEIIAYNEGGSKAGPRYNTDIAN
                     IVFNDWILEQADPNYVVVAPSYFILPIDFRPILKQHIAKKSKVTAVYQRVNNARNEYI
                     GRAIFDLNKDGHVINISRNKGEKDARNIGLEIYIFNRETFDFLIRDAQNISSFYSLSD
                     IVAYHALKDIRVDTYEYVGYMRCIDSYQHYYDYSFELLNYRIRRQLFLHNWPIYTVTH
                     DTPPAKYEATADVKNAFIANGSLISGMIKNSIISRNVKIEEGAVVENSIIFTDSIVRR
                     NKVISHAIIDKKAEVAVAAELAGTPEDILYVRKGDRV"
     gene            complement(10708..11838)
                     /locus_tag="PK460_03620"
     CDS             complement(10708..11838)
                     /locus_tag="PK460_03620"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOE54204.1"
                     /translation="MAKELIAMILAGGKGTRLYELTRKDAKPAVHFGGKYRIIDYALS
                     NCAHSGIDTVGVLAQYESISLSRYIGNGEKWGLNGIRSSTVTIAPRQTDSGSNWFIGT
                     ADAIYQNLEFIDSYNPENVLILSGDHIYRMDYSKMLETHLENNADLTISVIEVTLEEA
                     KRFGILSADEDGRITEFVEKPPKPKSTLASMGIYIFNYKVLREALVKDAADENSSHDF
                     GKNIIPTLLANNNRLFVHRFDGYWKDVGTIDSLWEANMDLIEMMDEDFYQADKHYFRL
                     FSEDTNSVPQYIGKKAKVTNSIVNQGAIILGEVEGSIISNEVFIEVGAVVKDSVIMPG
                     VTVGRNAYVNKAIVANDLELPEHSKYNEAGEKVLLIHEGVVQ"
     gene            complement(11843..12700)
                     /locus_tag="PK460_03625"
     CDS             complement(11843..12700)
                     /locus_tag="PK460_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54205.1"
                     /translation="MEAKFVDGIRAYHMRLRRRYLVELFVTISVFLAFCIALIVLDLL
                     SDFNMREPLFVPLLASATSISLLLFILTYFPIRFKRLQKALNLEFFQRAKTVKLELIP
                     NPLGGTKFDYYTLFNEAGMNVKSGAIKGVAPYEFNKRLFHVVYVYDDEKYSAIIHLPS
                     IDSPYYIQIHNGNFAPPEAYADNLILKIAYVSPYKLQYYASSSATNAKIYLRKENETR
                     FVKFIKIYQATYQYVTTFTDEYLKVEGLTSPGRLRLGEKYDNKHYHELLTRLRLLQAL
                     MSAMLERGK"
BASE COUNT         4021 a         2746 c         2073 g         4034 t
ORIGIN      
        1 tgtagaagag aaagccctta cattcgcttt ttaagtgtta ctttttcaag cttatcactg
       61 aagtattctt gccgtccaac ttttgcctgc ggtttgccgt taacaacgac aacatcacca
      121 gtaaatccga cttctttatt gttcacaata aaagagccta caccatagag attaacagga
      181 gcatttttgg ctttaaaata tttgattttt tcaggagtaa agccagaact tacgacgata
      241 tttacataat taaaaccttc tttatcaagt gcttcacgca cggcgaagac tagttcagga
      301 ctaacacctt ttagacttgg atcatttaaa ccttgacgca caaaatattc atcaagtaat
      361 gctttactat tatcaagacg aatcgagcct aatttcgcac caaaatgacg cgctactttt
      421 aaggcctctg tcactgtatc attatgatag tcaacaagcg cactgatctt ttcatttggg
      481 aaagttttgt gataaagttc acaagcctta attaagtcac cattagtcat cgcaattaaa
      541 gcatgtggca ttgttcccat ccctttaaca tttacccacg caccttgcgc atcagtggaa
      601 aactttttaa tccccgcaat ataactagcg tagccatctc ccgcctgtgt taaataatca
      661 tcttggcggt ctgccatact aaaaacttca gttggataag cagcttgcaa cacttcataa
      721 ctattggttg ctgctgtcgt tctccgcgtt aaaatgccat caataagact ttctaaatac
      781 gcaaaatctt tgtataaacc tgtaattttt aacactggtt cattcgcgtt aacaatatca
      841 ccatcatgac acgcttcaat ctttaatttt tcaggataac gcgcaaattc ttgtagtaaa
      901 gctagtgatt catctacacc acacgccatc acattatccc gccgttgaaa aaattgcatc
      961 gtcacgactg tgtccgctaa atgtttttca gcaatttcca ttgtttttac aaagtaattc
     1021 gcggtataaa aatgatcttt aatcgcactt ggaaatttaa aggttttgtt tgttaaacga
     1081 aacttccgat ctttgttttt catttcaatt tctgtcattt tcttatctcc tagcttcttt
     1141 aaatttatta atcttatcgc gatttaatgt tcttaacatg cgaattaata cttttttagc
     1201 atttttaata tcagtcgtat caacaacact agcggaagtg tgaatagagc gcgcacaaac
     1261 acagtgcgtc agtgttaaaa caccaacagc gtgtaaatga atggcccccg catcagtccc
     1321 acctggacta tgaaaatatt gatagggaac ttttagtgct tcagcacttt tttcttggaa
     1381 tgttaataat tccggaaaag caatcatcga gcgatctaaa taacgaatta aaattccttc
     1441 gccgagttgt ccttgttcac tgctactaat attatcgcgc gcgggcgaac aatctaaaat
     1501 gatcactaaa tctggattaa ttacattggc aatagttgtt gccccgcgta atcctacttc
     1561 ttcttgcacg gtggcaccga catataagtc atatggtaaa tcaacattct caaagtaccg
     1621 tgccatttca agcgccataa ataccccgta gcggttatca aaagctttag cgagtaagcg
     1681 cttgccacca tttaatactt caaaaggacc atctgcaaca atcatctgtc caacacgaac
     1741 accagcatta actgcgtctt ccttactaat aaagccgaag tcaaaatcaa gtttatcaat
     1801 cgttgtatta ccttctccgc cataatgcgg cgcggtcgca ccaacgcttc ccattaattt
     1861 agtgccatca tcaagcgtaa gacgtacgcg actcgctaac atcgtgttag cgttaatacc
     1921 gcccctagca tcaacacgaa taaaaccttt atcagtaatt tcacggacac taaaaccaac
     1981 ttcatctaaa tgaccagcaa ccataatgcg cggtgcctga gttactttac ttttctttac
     2041 accaaaaacc gacccgagat tatccgtaac gattgtaaaa ccgtattttt ctaattcgcc
     2101 tttgacaaac tttgctaatt cttgttctaa gccaggaatt gcatcgatct gcgttacttt
     2161 ttcaagtaat ttgagttcac gttttgttaa catataaaca cctatttctt tccaataatc
     2221 attttaattt taacaccttt attaaggaat ttgcgttcgt attccgtttt agcatcaaaa
     2281 tcataatcac ccttataatc ttttataaaa ataagctctt taaagggaac actacttaac
     2341 gagatgtaag aataatccgc gaagtcatca ttatccgttt taaaaattag tgtgccacct
     2401 tcttctaaaa gcttataata ctcattaacg agtgtcgagt aagttaaacg gcgcttttca
     2461 tggcgctttt ttggccacgg atcactatga tttaaaataa tgtaattaag tgaaagtgca
     2521 cttaattttg gtaataaata tttaacatca cctgttaaga ttaggatatt atttaagttt
     2581 gcgtccgcga gtttttgcgc ggcaacacta aggacgcttg catttaattc aataccaata
     2641 aaaaagtgtt cgggatagtt agtagccatt tctaaaagat aatcgccctt accaacacca
     2701 atttctagca ctaacggttt attaatatct ttaatttttg acaatgcgaa atcatcaacg
     2761 tgagtgtgaa acgctaatga ttttaagtaa tcaatagcac ctggtttaaa ttttaccctc
     2821 ataccttctt acctaagtaa tgaatagcac gagcgtaaat tgccatctgt gtataaaaat
     2881 catctaaata aatatgttcg ttagcggaat gaatatcccc gcttctacta ggaaaagcac
     2941 taccaaatgc aacagtatta ggacattctt tagcataagt tccgcccccg atagcaagtg
     3001 gcttactttg cgtgtcgcct gtttcatcgc gatatgcttt taagagtgtt tgaataaatt
     3061 cactctttgg atcaaataat aaatgtttac tacttctacc aatttctaat tcgacatcga
     3121 ttgtttgcgc taattttgct aaatgcgttt ccacttccac attttccgga taacggaaat
     3181 taacaacaaa acttaatttc ccgttttcat aatttagtaa tccgatatta tatgtcgtag
     3241 caccaagcag tgaggttgcg acatatgcat ccatagtttt accgtttgga tttttaaatt
     3301 tttccgctaa atgggtaaga aatggaagtt tatagaaagc acctaaatgt ttaaaagcaa
     3361 gtaccccggc gttcacacca agttcaggta aactaccatg cgcactttta ccaaaaattg
     3421 ttacatccat atttttcgat aacattttta ctgtatgctc aaccttatta tccgttaaat
     3481 atttaataaa atcttcatct tttaaaagcc gaatgacgac tttgtcaatc acactattag
     3541 cagcttcacc accagtaatc gtgcttattg gacctaaatc aatcatcttt gtcgcggtga
     3601 aattagtaat acctttttca ccatatatta gcggaaactc cgcatcaggt gtaaaaccgt
     3661 gtaccggtgc aggcgcatta tattcattaa agtaatagtg catacaactg ctaccccgtt
     3721 cttcgtcgcc accaattaca agacggacac tataattaat taataagtta ttattattca
     3781 aagttttaat tgcgtaataa gcagcaatcg ctggtccttt gtcatcactt gtgccacggc
     3841 cgtagagttt tccttcacga atatcagcgg taaatggtgg tgtatcccat tcgccagttg
     3901 ctggcacgac atcagcgtga gcaaaaacag caatattttt gttatacttt tcaccaacga
     3961 atatttcaac gacacggcca tcaataattt tcgcttcaaa tccgtcattt gtggcaagat
     4021 ttttcatata ctctaatgct tcgttaaccc ctttaccata tggtgtcttt ttcgtaactg
     4081 ttttttcgtc atataccgat ttaatactca caaattcttt taaagaattt aaagcatcgg
     4141 caaaataaac actggtaatc gttccataaa ttgatttatt tttcgtagtg aatttatcct
     4201 tatatttttc aaaaggaatg tataaaacat tgtataaaac aggaacaata acagttgcca
     4261 ctaaagcttc aagtaataca agcggagtaa gaatcgggaa aatatattcc ataaaagcat
     4321 ttacataacg caaatacatt gcaagtaaga caaaaaatga atgtaacagc atcataattg
     4381 ctgaataagg gaatatcagg aaacgtttta atttaaaact agtgcgttca ttaaatgaaa
     4441 ccgctgaaaa tattgcggta caaaatccaa ataatatccg cggcacaata ctaattaaag
     4501 gatcaacaaa taatgcgtcc ccagcagtac cacgaataac agcaattaaa aaggacgaaa
     4561 aaccaaaagc agcgccataa agcacaccgc gtctaggtcc taaaagtgcc gctcctaaaa
     4621 ttacaggaat tggtaagatt gtcgccgaaa tccccccgat ttgaataaag cctaaatacg
     4681 ggacaagact taacactaaa ataagggcaa taaataaccc atcaattgca atatcactta
     4741 ctaacttttt atttttcatt gccatttacc aacatgtcat ataacccgta atgcactaaa
     4801 tgtggaacat aatttacact ttcatccaca gcacctttaa tatagcgtgc attaccacca
     4861 gttaataaca caattggatt atctcctact tcttttttta ttaaattaat aaacccgttc
     4921 atctccccta ataaaccata taataaaccg ctattaagcg agtcgggtgt atttttaccg
     4981 agtactttcg cgggtaaagt aaggcggaca ttaaataatt gtgctgcatt ttttactaaa
     5041 cctttaaacg atacttccac accaggacta attgccactc cgtgaaaaac atgtttatca
     5101 tcaacataaa aatatttatt agccgtccct aaatcaataa caataaaagg acctttccca
     5161 aatttataag caccaacagc gcaagccaca atatcactac caacgctttc gggaacatca
     5221 acatctagtc tcacactagt tttaggattt ggtaaaacaa tctcagcatc gactcctaaa
     5281 atttctttaa tcacaaatga cattggataa tttaacttcg ggacaacact agaaataagc
     5341 gctttcttaa atgtactgtg cgcatattgc tttttaaatt cgttaatgat gggacgaacg
     5401 gtatcaaagt ttgcttgtgc cgtttctttt agcgctttcg gcgtttcacg attaaaaaca
     5461 tcaacaattt caccagcgtt aaatagcgca atactaatta cactatttcc gatatcaact
     5521 gttaaataca taagctctcc ccaccacttt tactaaataa gtgcgtaata tattataata
     5581 tataattcct aagaagcaac gatatttacg attttatttg ggacgacaat gacccgtcta
     5641 atcgttaaac catcaataaa acgctggaca ttttcattag tgagcgcggc cttttcaagt
     5701 tcttctttac tttcattaag tttagcttta attgtcgcgc gtaatttacc attaacttgc
     5761 acagcaattt caacttcatt tacgactaac tttgcttcat cgtaagttgg ccacgcttca
     5821 taagttattg tgtcaacatc aacgaattgt gagtaaagtt cttcgcctaa atgtggagcg
     5881 attggataaa gtaatttaat taaaccgacg taataaggaa tataaacttc acgcgcttta
     5941 tacatatcat taactaaaat catcatctgc gcaatcgcgg tattaaacgc taatttttcg
     6001 tagtcttcag tcactttttt aacagtgtag tgatacgagt aatcaagctc accattattc
     6061 gtatccacaa gttttccagg gaaatcttca ctattaattc gtgcgacacg atttaagaaa
     6121 cggagcgcac cttctaaccc tgtatttgac cacggtaagc ttgcttcaag tggacccata
     6181 aacatttcat aaagccgcaa agcatcaaca ctatattcag caataatatc atcaggttta
     6241 acgacattac cacgtgactt cgacattttt tcaccatttt cccctaaaat catcccttga
     6301 tggaataatt tagtaaatgg ttcttttgta tttacaacac ctttatcata taaaaactta
     6361 tgccaaaaac gcgcataaag taaatgcaag accgcgtgtt ccgccccacc aatataaaga
     6421 tcaacaggga gccagtgctt aataagttct ggatcagcaa ttttttcatc attatgcgga
     6481 tctaaataac gaatataata ccagcatgac cccgcccatt gcggcatcgt atttgtttcg
     6541 cgtttaactt tttcaccctc ataagttgta ttaacccaac tagttgcaag cgcaagtggc
     6601 gactcaccag taccgctagg tttatattcc tttaacggcg gtaaagtaag cggtaaatca
     6661 cttaaaggta cagggataaa agtgttatct tctttaatca taattggaat tggttcaccc
     6721 cagtagcgtt gacgtgagaa taaccagtcc cgtaatttat aattagtcgt tactctacct
     6781 actcctttat tcgttaagtg ttcattaagt acttttttac tctcttcaat attaagcccg
     6841 ttaataagcg gactattaat atggggggcg tcatcaataa aagcattttc actaatgtcg
     6901 cctgctaaca cttgaaccat cggtaagcca tggcgcttag caaattcgta atcacgttcg
     6961 tcatgcgctg gaaccgccat tactgcgcct tcaccatatg accctaaaac ataatcagca
     7021 acataaagcg ggatttcttt gttattaagc ggattaatag cgttaactcc taaatacacc
     7081 cctgatttat ctttattaag ttctgtccgt tctaagtcgc tcttatgact agcgctttca
     7141 atataacgct ttacttcttc tttttgcgtg tcactaacaa cttttaacac aagcggatgt
     7201 tcaggcgcta aacatgcata agtcgcccca taaagcgtat caacacgcgt tgtaaagaca
     7261 tcaaactcgt aatttgtatt cgctacttta aaagtaatcg ttgtgccttc gctcttccca
     7321 atccagttac gttgcatatc aagcgtagaa gcgggccaat ctaaaccgtc taaatcactt
     7381 aataaccgct ccgcgtactc cgtaatgcgt aataaccact ggcgcatcgg tttacgaatc
     7441 accgggaatc cgccaacttc acttttaccg tcaattactt cttcatttgc gagtactgtt
     7501 cctagttcag gacaaaagtt aacagggata ttatcgacat aggctaagtc gcttttaaaa
     7561 agttcagtga aaatccactg tgtccattta taatactttg gttcactagt agaaagttct
     7621 ttactaaaat cataactaaa gccaagcgac tttaattgcc gtctaaaatt agcgatattt
     7681 tcgttagtaa aaatagccgg atgattattc gtgcgaatcg catattgttc cgctggtaaa
     7741 ccaaaggcgt caaatccaat cgggtgtaag acattatagc cttgcatccg tttaaagcga
     7801 gcgacgatat cagtcgcggt atacccttca acgtgaccaa catgcagtcc gtcgcctgaa
     7861 ggatagggaa acatatctaa cacataatac tttggtttac taaagtcata catatctgta
     7921 taaaatgtgt tattttcttc ccaatatttt tgccacttct tttctgcggc acgaaaatca
     7981 taagccataa tgttacctcc aaaaataaaa ggtgttacca aagggcggaa agtccgcggt
     8041 accaccttat tttataactt catttcctat taatgctagg gcacagcgag ttaactgaag
     8101 gtgagacata acttaatata agttatttat tcttcactcg cgataaaatt atatcacaat
     8161 aattatttaa cgatactttt atagagcgct aggtattttt taccagcttc agaccagtca
     8221 ttatttttct ccatcgcatt gcggcgaagc tttttaaaga ccttttgatt tttgtattgt
     8281 tcaagcgcat aattcgtcgt gagtttcatc cagtattcgt cataattatc aaagacaaaa
     8341 ccatcagcga catcttcatt agtgccatca tacccaatta ccgtatcttt taaaccaccg
     8401 gtagtacgca caattggcag cgtcccgtag cgctgggcaa tcatttgccc aataccgcac
     8461 ggctcaaata aacttggcat gaagaagaaa tcactcgctg cgtaaacgcg gtgcgcaagt
     8521 ggattattgt aaccgatata aattgccata cggtctggat agcgagcgcg cagttcttca
     8581 agcgcttgtt catacccatg ttccccgcta ccgacaacga caacattgcc acctttttct
     8641 aatacttcaa caatagcggg aagcactaaa tcaatgcctt tttgccacgt taaacggcta
     8701 accatcgaaa atagcggtgt atcaacatca cctttaatac ctaattcttt aaatagtaca
     8761 agtttgtttt tcgtcttaat tcctggtcta gttttaccat atctaaacgg taaatgttta
     8821 tcagtctgtg gattaaattc atcgtaatcg atgccgttaa gaataccgct gaagtcattt
     8881 ttccgtaatt ttaaaatacc gtctaaccct tttgaccctt caggggttaa aagctcttcc
     8941 gcgtgcgtag gactaacggt attaatttta tcagcgaaga caatcgcagc tttaagcgca
     9001 ttaaccttgt cacgataacg taatgttccg tcatcaaaat actccttccg aatatcataa
     9061 aaatcaggga gtaaatccgg atggaattca ccttggaatg ctggattatg aattgtgaca
     9121 acaaatttca ttttattaaa gaattttgtg cgtgaatttt gaattttgat taacgctggt
     9181 accatgccgc catggtaatc gtggacatgg acgacatctg gtttaatttt taatgcttgc
     9241 atcatattgc gaacagcgac actaaaaaac gcaaagcgtt ccgtatcatc gtcataccca
     9301 taaaaccctt cacggccaaa atagtattca ttttctaata aataaaatgt aataccgtca
     9361 ataaatgttt tatacacttt agcgttttga aagcgccagc ctaaagggac ttggacattc
     9421 gcgacaaagc ggatttcttg atcaggactc cgtttaatat tgccgtaaaa tggcatcact
     9481 acacttactt cttccccaag aatcgcaagt tccttactta aggcatagac gacatcggct
     9541 aagccaccag tttttgcaag tggactactt tcacttgcta ccattaatat tttcattaca
     9601 ctcgatctcc ttttctaaca tatagtatat cttccggcgt acccgctagt tcagcagcca
     9661 ccgccacttc cgccttctta tcaataatag catgactaat cactttattg cgtctaacga
     9721 ttgaatcggt aaagataatg gaattttcca caaccgcccc ttcttcaatc ttgacattcc
     9781 gtgaaataat cgaattttta atcatcccgc tgattaatga tccgttggcg ataaacgcat
     9841 ttttgacatc agccgttgct tcatacttcg ctggtggggt atcatgcgtt acggtgtaaa
     9901 tcggccaatt atgtaagaag agttgacgac ggatacgata gttaagtaat tcaaaactat
     9961 aatcataata atgctgataa ctatcaatac agcgcatata cccgacatac tcataagtat
    10021 cgacccttat atcttttaag gcatggtaag caacaatatc acttaaggaa taaaatgagc
    10081 taatgttttg ggcatcacga atgaggaaat caaatgtttc acgattgaaa atataaattt
    10141 caagtccgat attgcgtgca tctttttcac ctttatttcg tgaaatatta ataacatgac
    10201 cgtccttatt taaatcaaag atagcgcgtc caatatattc atttcgcgca ttattaacgc
    10261 gttgatatac cgctgttact tttgattttt tagcaatatg ttgctttaaa atcggcctaa
    10321 aatcaattgg taatataaag taacttggag caacgacaac ataatttgga tcagcttgct
    10381 ctaaaatcca gtcattaaag acgatattag cgatatccgt gttataacgc ggaccagctt
    10441 tcgaaccgcc ttcgttgtaa gcaataatct ctaaccctaa cttagtgtta atgttaaaag
    10501 tattacgact accaaaatgt tttaatactg aatcaggatg attatcgact agtgttgcaa
    10561 tccggtcaaa tccgctattg ctaaagttac taagcataaa gtccataatc gcataacgcc
    10621 ctaaaaaact aacaacagca gtcgaacgcc gcgtcgttaa tggtccaagg cgtggggcat
    10681 ggtgtaaatt aagataagct aaaactttca ttgcactact ccttcatgga tgagtaatac
    10741 tttctcaccc gcttcgttat atttagaatg ttctggaagt tctaaatcat tagcgacaat
    10801 tgctttatta acataagcat tacgcccgac agtaacgcca ggcataatga cactgtcttt
    10861 tacaactgcc cctacttcaa taaaaacttc attactaata attgatcctt caacttcacc
    10921 taaaataatg gcgccttgat tgacaatact attcgtgact ttcgcctttt taccaatgta
    10981 ttgcggcaca ctatttgtgt cttcactaaa aagcctgaag taatgtttat cggcttgata
    11041 gaaatcttca tccatcattt caattaaatc catatttgct tcccataatg aatcaatcgt
    11101 accaacatct ttccaatacc catcaaagcg atggacaaat aagcgattat tattcgctaa
    11161 taaagttgga atgatatttt taccgaagtc atgggaacta ttttcatcag cggcatcttt
    11221 aacgagtgct tcgcgtaata ctttatagtt aaagatataa atacccatac tcgctagtgt
    11281 tgattttggt tttggtggtt tttccacaaa ttcagtaatc cgtccgtctt catcagcgct
    11341 aagaatccca aagcgcttag cttcttcaag tgtgacttca atgacagaaa ttgttaaatc
    11401 agcgttattt tctaaatgtg tttcaagcat tttcgaataa tccatccgat aaatatggtc
    11461 accacttaaa attaaaacat tttcgggatt atacgaatca ataaattcca agttttgata
    11521 aatcgcatca gcagtaccaa taaaccaatt actgccgcta tcagtttgcc ggggcgcaat
    11581 tgttactgta ctacttctaa tcccgtttaa tccccatttt tcgccgttac ctatataacg
    11641 actaagacta atcgattcat attgggctaa gacaccaact gtatcgatgc cactatgcgc
    11701 acagttactt aaggcatagt caatgatgcg gtattttccg ccaaaatgca ccgctggttt
    11761 agcgtctttg cgggttagct cgtagagacg tgtcccttta cctcccgcta aaatcattgc
    11821 gataagctct tttgccatcc ttttacttcc ccctttcaag catcgctgac attaaggctt
    11881 gtaacaagcg taatctcgtt aaaagctcat ggtaatgttt attatcatat ttttcaccta
    11941 gccgtaatcg acccggtgaa gttagccctt caacttttaa atattcatca gtgaaggttg
    12001 tcacatattg ataagtcgcc tgataaattt tgataaattt aacaaagcgc gtttcatttt
    12061 cttttcgtaa atatatttta gcatttgtgg cactacttga ggcatagtat tgtagtttgt
    12121 agggagaaac atacgcaatc tttaaaatta aattatcagc gtaagcttca ggtggagcaa
    12181 aattaccgtt atggatttgg atgtaataag gtgaatcaat acttggcaag tgaataatcg
    12241 cactatattt ttcatcgtca tacacatata caacatgaaa aagccgctta ttaaattcgt
    12301 aaggcgcaac acctttaatc gccccacttt taacattcat gcccgcttca ttaaaaagtg
    12361 tataataatc aaacttcgtg ccgcctaacg gattgggaat aagttcaagt tttactgtct
    12421 ttgctctttg gaaaaactct aaattaagcg ctttttgcag ccttttaaag cgaattggga
    12481 aataagtaag aataaataag agtaaggaaa tactggtagc actcgctaat agcggaacaa
    12541 aaagtggttc acgcatatta aagtcactca agagatcaag gacaattaag gcgatgcaaa
    12601 aggcgagaaa aacgctaatg gtaacaaata gttccactaa gtagcggcga cgtaagcgca
    12661 tgtgatatgc tcttattccg tcaacaaact ttgcttccat aattctcctt tctttgcggt
    12721 taagttaagt cctcttctct cgcaaataat tgcactgtgc tatgattttt accgcgtaat
    12781 acaacaagtg tttcatcgac taaggggcta atatcagcca ctttatcact tggggcacta
    12841 ataattgctt gtaagccaaa gcggcgtaat aagc
//
LOCUS       DAOSLP010000030        12838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_4937_length_12838_cov_8.506532, whole genome shotgun sequence.
ACCESSION   DAOSLP010000030 DAOSLP010000000
VERSION     DAOSLP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4937_length_12838_cov_8.506532"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..677
                     /locus_tag="PK460_03630"
     CDS             159..677
                     /locus_tag="PK460_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOE54206.1"
                     /translation="MNDEKKYVVIVERLARRGVNEMDIAKITYKLQTKYIPDLEMDIC
                     LDHVKRVLKKREVQNAILTGLELDILAEKGMLSEPLQTIIASDYGLYGIDEILALSIV
                     NVYGSIGSTNFGYVDKVKPGIIGELDKKTNGKVVNTFLDDIVGAIAAAAASSIAHKYA
                     DADFDTELYKKQ"
     gene            complement(735..1118)
                     /locus_tag="PK460_03635"
     CDS             complement(735..1118)
                     /locus_tag="PK460_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HOE54207.1"
                     /translation="MDKTKRNRLIMWLVLSGVTLIAATLILVLRKDYSTRGFSDATFI
                     PGVVVLFLFLLRLVRDAGTFDLVSYSISRIFQGTKEKSLDGMKTASEYVEGKRQERLK
                     KDRYYLPYIIIAVAFIVAGVILAYI"
     gene            complement(1286..2017)
                     /locus_tag="PK460_03640"
                     /pseudo
     CDS             complement(1286..2017)
                     /locus_tag="PK460_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2555..2932)
                     /locus_tag="PK460_03645"
                     /pseudo
     CDS             complement(2555..2932)
                     /locus_tag="PK460_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2925..3842)
                     /locus_tag="PK460_03650"
     CDS             complement(2925..3842)
                     /locus_tag="PK460_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002481942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE54208.1"
                     /translation="MKAESKPVLTELKAKLKVAQKKAKAEVKAYHKEIKAEYKKERDT
                     LRAELRATKNEERRKELKEALIAAKRNYIDKTRVTKQVAHDMAVKVMEEVGIPNAARR
                     FRQYPFEFSGGMRQRIVIAIALIQNPDILICDEPTTALDVTIQAQILELILRLKRERQ
                     LSVIFITHDLGVVANMADRVAVMYAGRIVEYGKVEEIFYNPKHPYTWALLSSIPDVDT
                     KERLEAIPGTPPNMVYPPKGDAFAPRSKYAMAVDFIEEPPYFKVSETHYARTWLLDPR
                     APKVEMPKIVSERIRISLERAGKGGEFDA"
     gene            complement(4029..4475)
                     /locus_tag="PK460_03655"
                     /pseudo
     CDS             complement(4029..4475)
                     /locus_tag="PK460_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(4475..6442)
                     /locus_tag="PK460_03660"
     CDS             complement(4475..6442)
                     /locus_tag="PK460_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE54209.1"
                     /translation="MSEKKQLITEKDFVFVQQDEKIHDLKFDTKPTTYFKDAMRRFVK
                     NKSSVVAGVIIGILILLALFVPIFNKNDIVTSQEDYRFLPPRWPGFQNASVLNGRRYV
                     ENIIYDATDEDETKHHPVGYDNEAIVGEVKLTEGTTNFASPYASGGKVLISSDRSSFA
                     TGIQTNKITADFALNDYYLEIVTDTELNRDNNYEIYVLVYYNGPNSDATLVVLQEQSM
                     KYGTFTTNLSDVVLSSRPAAILEDEFTVQIGVYVPATNSPDGTRAQLYLNKLAVTTTA
                     EENPFELHNYTDANELLLRRSDKEKIPYRYQTVISGNQMLYEGRVIRASFEYDYYKAV
                     YGEVNRIIGISDLLNARRDGKVKFDLDPAHSDHTKPDFNYQIVMDTFEVVNDKTSQYR
                     EITFAHPRQTVKAGMTTIYVYNYDVVVSRYREMGYTREPFFLFGTNQRGQDFFKIVFN
                     GLRTSLILGFSAAAINIFIGLVWGAISGYYGGTVDILMERFTEILGGVPWIIIMTLTI
                     LHLGNNFGVFLLALILTGWIGTASVTRSQFYRYKRREYVLASRTLGAKDSRLIFKHIL
                     PNSIGPIVTGAVMMIPGIIFSEATISYLGLGLQGLTSLGVALSEVQAYIYTDPYLIVT
                     GSLIIALLMISFNLFGNGLRDAFNPSLKGVE"
     gene            complement(6442..7476)
                     /locus_tag="PK460_03665"
     CDS             complement(6442..7476)
                     /locus_tag="PK460_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE54210.1"
                     /translation="MGKYILKRVGLVFVTAFIILSLTFILMKLLPMDRPIGLAPDQLR
                     YYNTQVRLGYMVQVQGPLMPGQTVDITVNDGGKLYYYNQVPVMRQYLSWLRNVITRWD
                     WGVSSRIERNVPAIEIIARKLPVSMKLNIIALFVSVPLGFLFGIIAALQKNKLPDHII
                     STGVMIFISIPSFVFITFLMLFFAYKLEWLPSQWPPEGLSWQMQAKAYVIPVMALSFG
                     TIAGFTRYTRAELTEIMSSEFLLLARTKGLTKRQTVIRHALRNSLVPIVPMIIGQFIG
                     ILSGSMILEALYGIPGIGSLFVTAITNKDYNVVMVDMALYTTIGLFAGLLVDLSYGIV
                     DPRIRMGARK"
     gene            complement(7608..10076)
                     /locus_tag="PK460_03670"
     CDS             complement(7608..10076)
                     /locus_tag="PK460_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE54211.1"
                     /translation="MKKKKLLIVGLLGLVALSGCKKPSSSVEAPSEVSSEDVLVETVP
                     AVSGVRTFAGEKPAAIKDRKDILTLLESYALDTFIGGIPYRDNSAVVLYSSDLKMPAD
                     DYVVGYGFGVGEAEILAPLTGSQVAPERAKYYHTWTSQDPGTINYMDSQESVTGDLHA
                     LISSGYWGTKFNADKTGYEWYPLLANDVERPIPLNFDEATGTATKWRVPLKVGGDLKY
                     NTLGVHSEFAGLEVKLEDYLVPFKLMVETGWFRGTDLASKDSGFVGVAEELQKPVGER
                     SIERVQGIKLNVAEQAMEFEFNSAKSPFYAMYNLASGLFMPIPQSFIDKIGVENYGKA
                     DIDNTLSLGVYNLEIWEKDKQVVFKKNPLWIEADRYTHEGYVFTILDDSNTAFQQFLA
                     NNLHSAGVPASYLKEYRNDERARQTKGDTTWKLQVNAANQERWNELFGEEGSIAPGGE
                     WELKPVMSNLDFLNGVYYSLNRDQLADTTGSRPAMGFFSEAYMIDPESGVSWRVSEEG
                     QAVLEGRSTETNGFNKEAAGQLFNKAMNDLVAEGKYTRGTAENPTVISPRIHFQSVQQ
                     TVDEGGPLKRMIEAAFNDAVEGFKLDIELFATQNWMDAYYAMMFGEFDFAFGTISGNT
                     LDPLSFMLTLCSDNRSGFTLSWGHDTGHTYNSYEVGGLVFKGERYSYDALLESTLGLT
                     IIEEGKTVPSFSLKGLDFGFNEADPTKYDFVAQGTYYVDPAGEYVITPDPNGTKEETG
                     GFLALVFYNKEGEFQSIVPLPATTFENGAWTVVAEGISVPEDAHYMDIYISTIHTYKG
                     IPEYRANLYELLWKSFAPEAPAGE"
     gene            complement(10302..11420)
                     /locus_tag="PK460_03675"
     CDS             complement(10302..11420)
                     /locus_tag="PK460_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54212.1"
                     /translation="MAQKSVTFRTVFKRYKRERRSIKSYLLFTLAFLAFSFVTVYLNS
                     TELALLGAILLVFVIFPLIVVFSEYTALIESGAPAPRNSRAYFDLYKNTYRSGRLRHI
                     FNLRNVVVFLIYFLVGMFIAGLGMTLFIYFFDKPVYDQMFNLLLEFQGTSTPDALNVL
                     VTKIELLLEPYIPSQILAYNLVATLGLIFIIVKGVFNIYLSIFIEHRPTTSFIVLRNT
                     FVGDAETKSSLRRVQTGIVLIVFSLYTLFNVGGYFLLQAINPHGPLFLQAELLGLVVL
                     TIALPFTTRFTFYLYHSVMEKKREQILRFAIVELREIIKNPNLPEQTKTYITQVLKVR
                     EQEYAALTAVEAEQKVVDEKAPASNDDTTNEEIDSDNK"
     gene            complement(11402..12061)
                     /locus_tag="PK460_03680"
     CDS             complement(11402..12061)
                     /locus_tag="PK460_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HOE54213.1"
                     /translation="MLFAVFGVKEYVILALAVVLVLFALVNIFLFFRKKILQRFALLR
                     YFYPVVRKTAVYYDYYLINKLEIQLVSNESLFIDHMLFGNKYIYVINDYLFKGVLTGN
                     AQDSKWILKDKKGQEIMVDSPLLKNKKLVEKLSLRTSLDHATFIAITLVSKDTKITDL
                     EVNDSSNFVIDTRDFMKLILKIESRDIAPLNPEDLLKRVHEFDELNLRKKRGRKWRKN
                     R"
     gene            complement(12064..12777)
                     /gene="deoD"
                     /locus_tag="PK460_03685"
     CDS             complement(12064..12777)
                     /gene="deoD"
                     /locus_tag="PK460_03685"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOE54214.1"
                     /translation="MGTPHINAKKGDFAKTVLMPGDPKRAEWIANTFLTDVKRVTDVR
                     NIFGYTGYYKGKRVSVMASGMGLPSIGIYCHELYTYYGVETIMRVGTCGTYQPEVDLF
                     DIIVAIGSCTDSNWAAQFNLLGGTFSAIGDFDLAVKAKEVANASGINVHVGNILSADI
                     FYDNDPNSWKKWANLGVLGVEMESYALYTTAAKLKKKALCLLTVTDSFIDHSKKATVE
                     ERQTGLEKMIRVALEIAEE"
BASE COUNT         3859 a         2508 c         2336 g         4135 t
ORIGIN      
        1 atttaaaagt ttcctaatct ttatttgtaa agataagtat gtcatctaac aataaagtaa
       61 atcactaatt atttttttaa ttaggaatca ctttatttcg ttatatgtat ataattatta
      121 ggaggtaaaa aaatatggat tacgaaacaa gaaatcgaat gaacgatgag aaaaaatatg
      181 ttgtaattgt agaacgccta gcgcgtcgtg gcgtcaatga aatggatatc gctaaaatta
      241 cttataaatt acaaaccaaa tatatcccag acttagaaat ggacatatgt ctcgatcacg
      301 ttaagcgtgt attaaaaaag cgtgaagtac aaaacgctat tttaactggt ttagagcttg
      361 atatacttgc ggagaagggc atgcttagtg aacctttaca aacgattatt gctagcgatt
      421 atgggcttta tggcattgat gaaatcttag cgttatcaat cgttaatgtc tatggttcaa
      481 ttggttccac taactttggt tatgtcgata aagtaaagcc aggcattatt ggtgaattag
      541 ataagaaaac aaatggtaaa gtcgttaata cattccttga tgatatcgtt ggcgctattg
      601 cggctgcggc cgctagttcg attgcgcata aatacgctga tgcggacttt gatactgaac
      661 tttataaaaa gcaataatca caaaacaaaa cacgaaaaaa gcccggttca atcgggcttt
      721 tcattaactc caagttaaat gtacgctaag attactcctg cgacaataaa agcaacagct
      781 ataatgatat atggtaagta ataacgatct tttttaagtc gttcttggcg tttaccttca
      841 acatattcac tagcggtttt catgccatca agtgactttt ctttagtacc ttggaagatg
      901 cgcgagatac tgtaggacac taagtcaaaa gtgccagcat ctcttactaa tcttaagaga
      961 aataagaata agacaacaac tcctggaata aacgttgcat cgctaaatcc gcgagttgaa
     1021 taatccttgc gtaacacaag aattaaagtc gcggcaatta atgtaactcc gcttaaaact
     1081 agccacataa ttaagcgatt gcgttttgtt ttatccatcg ttattttttc ggcttgtcgg
     1141 ctttagcttt agcagtgctt ttcgctttgg gcttcgctgc ttttttgggt tcagcctttg
     1201 gctccgcctt ctttttagcg gctctagccg cttctttttt ggcttctgct tctgcttttt
     1261 tatctaactc tttaatttct tcgcgataac gttccatttc ctcactattt gctaagacaa
     1321 agtgacctgg taagatttct tgtagtgttg gtttttcttc acgataatcg tgagcggtaa
     1381 acgggttata taaaatccgt tcacgggttt tttcactata aggatctggt tttggaatgg
     1441 cgcttaggag tgcttttgta tatggatgta atgggtgttt aaataactca tcactcgttg
     1501 ttaattcaac aatattacca taatacataa ctgcaatccg gtcacagaaa tatttaacaa
     1561 cacttaagtc gtgagcgata aagagaatcg ttaagccaag ttcatcacgt aagtcgttaa
     1621 gtaagttaat aatttgtgcg cgaatcgaaa cgtcaagcgc actgactggt tcgtcggcaa
     1681 taatgaattc aggattcata atcagcgctc tagcaatccc aatccgttgc cgttgaccac
     1741 cactaaattc gtgcgggtac cgcgaagcat agtcgctaat taatccaacc ttttctaagg
     1801 attcgataac tttcttcgag ttttcagcgc ggtctttcat cccttgaatt tggagacctt
     1861 cttgaataat atcttcaaca gtcatccgag ggttaagact atcaattgga tcttggaaaa
     1921 tcatttgcat cttattcatg atacttgtat caacatgttt catatcatag ttaatttgtg
     1981 caatcttttc tttttgtgtc gagacgatat ccgcaagttt tgcctttaag tcagcaataa
     2041 cttcgtttgt tgctgtttta agttcactaa cacgcaagtc ataagcttct tttgctttaa
     2101 cggcaaatac ttttgtctct gccgcaagtt cttctttgac tgttggtgaa agatttttat
     2161 caccagcttt aataactgct tttaattcta aaacacgttt actatcttta tcatgttcct
     2221 taacaaaagc ttttttaatt atttcaaact tatttgtttc acgtttaagt tgaccttcat
     2281 actttttatt aatttccgca acgataatat cattatcatc ttgactatcg tcactacggt
     2341 cacggagttc ttctttaagt gcttgtttta agtcacgaac tttagtttta tagttatatt
     2401 catcagtggc aaatgtattt ttgcgttctc taattgtcac taaaagggca gcaaactcac
     2461 gcttagcagc ggctagttct ggtgaacttc ttgctaaaat atcaagttca gcatctttaa
     2521 tttgttgttt aagttcagct ttttcccgct taatatcagc gcgtaatact ttacgcagcc
     2581 gtttaatttc acgtctagtg cgaattgttt tccatttaat ttctttttcg ttccagcgtg
     2641 aaccagcgtt aatacggtag cctttaaaat aaatgctccc acttgtgacg tcatatagtt
     2701 taataatcga acgtccggtt gttgttttac cacagccaga ttccccgact aaaccaaaga
     2761 cttcaccttt tttaatatca aaggaaatat catgaacagc ttttgtgcgt aatttattag
     2821 caccgcgtcc cgaagtaaag aattgtttta aatgccgtac actaagaata atatcttgat
     2881 cgacaagttc ttgtttaaat gttacttcac gtcttttacg ttttttaagc atcaaactct
     2941 cctcctttcc ccgctctttc aagtgaaatg cgaattcgtt cggaaacgat ttttggcatt
     3001 tcgacttttg gtgcacgtgg atcaagtaac catgtccgtg cgtagtgcgt ttcacttact
     3061 ttgaagtacg gtggttcttc aataaagtcg actgccattg cgtatttaga acgtggggca
     3121 aaagcatcac ccttaggtgg gtaaaccatg ttcggtggtg taccaggaat tgcttcaagc
     3181 cgttcttttg tatcaacatc aggaatactt gagagtaatg cccaagtata cggatgtttt
     3241 ggattgtaga agatttcttc aactttacca tattcgacaa ttcggcccgc atacatcacg
     3301 gcgacgcggt ccgccatatt ggcgacaaca ccgaggtcgt gcgtaataaa gataacggat
     3361 aattgccgtt cgcgtttaag tcttaaaata agttctaaga tttgcgcttg aattgtaacg
     3421 tcaagcgctg ttgtgggctc gtcacaaatt aagatgtcag gattttgaat taaggcaatc
     3481 gcaataacaa tccgttgccg catcccacca ctaaattcaa atgggtactg acggaagcgt
     3541 cgggcagcat taggaatacc gacctcttcc ataactttaa cggccatatc gtgtgcgact
     3601 tgttttgtca ctcttgtttt atcgatatag ttacgttttg cagcaattaa cgcctctttt
     3661 aattctttac gacgctcttc gtttttcgta gcgcggagtt cagcgcgtaa tgtatcgcgt
     3721 tcttttttat actcagcttt aatttctttg tgataagctt ttacttctgc tttagctttc
     3781 ttttgcgcaa ctttgagttt agctttaagt tcggttaaaa ctggtttact ttctgctttc
     3841 acgcgtctaa caagcgcacg atgttctaaa tcagcagctt tttcttcttc aattaaagcg
     3901 tctaacttaa cgcgtttttc ttctttcgtt aatgctttgt cagcgcgaat atcttgtgct
     3961 ttaaatttat taccgacttt taagttacga agtttaaagg cactagtatc gatttcatca
     4021 cggaattttg tgacagcgtt acgataagca acattttctt cataaatcaa gtcttcatac
     4081 attaatttaa gcttattacc attaataata atcgcttcag taatttgttt accaatacgc
     4141 atcaaaggat ttaagcttga gagtgggtct tggaaaatca ttccaatctt tttcccacga
     4201 atacggtgga attcttcttc actgactttc gttaagtctt caccaccata cattattctg
     4261 ccaccttcaa cacgaccatt aaccgctaaa atgcccacga tcgttttagc agatacgctc
     4321 ttacctgagc cactttcacc aacgatggcg agaacttcac cacgatagag ttcaaaactt
     4381 accccacgaa cagcgtgcaa ccggccatag tcagtactaa aggaaatcga taagtcttca
     4441 actttgatga ctacttcttt atctgtatgt ttcattattc gactcctttc aatgatgggt
     4501 taaatgcatc acgaagtccg ttaccaaaga ggttaaagga aatcatgagt aaagcgataa
     4561 taagtgaacc ggtaacaatt aagtatggat cagtataaat gtatgcttgg acttcactga
     4621 gcgcgacccc aagacttgtt aagccttgta acccaagccc taagtaacta atcgtcgctt
     4681 cactaaagat gatacctgga atcatcatga cggcaccggt cacaattgga ccaatggagt
     4741 taggtaagat gtgcttgaaa attaagcgac tatctttcgc cccaagtgtc ctacttgcta
     4801 aaacatattc tcggcgttta taacgataga attgactacg cgttacactt gccgtgccga
     4861 tccagcctgt gagaataagc gctaaaagga agacgccaaa gttattccct aagtgtaaga
     4921 ttgttaaggt cataataatg atccacggta ccccacctaa gatttccgta aagcgttcca
     4981 ttaaaatatc aactgttccg ccgtagtaac cactgatggc accccagact aaaccgataa
     5041 aaatattaat agcggcagca ctaaagccta aaattaagga tgtgcgtaaa ccgttaaaga
     5101 caatcttaaa gaaatcttga ccacgttgat tcgtgccaaa aaggaagaat ggttcacgtg
     5161 tgtaacccat ttcccgataa cgagaaacaa cgacatcata gttatataca taaattgttg
     5221 tcatcccagc tttaactgtt tgccgtggat gggcaaaagt aatttcgcga tattgtgatg
     5281 ttttgtcgtt tacaacttca aatgtatcca tcacgatttg atagttaaaa tctggttttg
     5341 tatgatcgct atgggccgga tcaaggtcaa atttaacttt gccatcacga cgggcattta
     5401 gtaaatcact aatcccaatg atacgattaa cttcaccata aactgcttta tagtaatcgt
     5461 attcaaaact agcgcgaatg acacgacctt catagagcat ttgattacca gaaataactg
     5521 tttgatagcg atatggtatt ttttctttat cacttcggcg tagtaataat tcattcgcat
     5581 cagtgtagtt atgtagttca aaaggatttt cttcagcagt agtcgttacc gctaatttat
     5641 ttaaatataa ttgcgcgcgc gtaccatctg gactattagt tgcgggcaca taaacaccaa
     5701 tttgtacagt gaattcatct tctaatatcg ctgcaggacg actagataat actacatcac
     5761 ttaaattagt agtaaaagta ccatatttca tactttgttc ttgtaatacg actagtgtcg
     5821 catcactgtt aggaccgtta taatacacaa gcacataaat ttcgtagtta ttatcacgat
     5881 taagttcagt atcagttaca atctctaaat aataatcatt aagagcaaag tctgcagtaa
     5941 ttttattcgt ttgaatacca gtagcgaaac tgcttctatc actagaaata agcacttttc
     6001 cgcctgatgc gtatggcgaa gcgaagttag tggtaccctc ggttaatttt acttcaccaa
     6061 caattgcttc gttatcgtaa cctactggat gatgtttagt ttcatcttca tcagtcgcat
     6121 cataaatgat gttttcaaca tagcggcgac cgtttaatac tgaagcattt tgaaaaccag
     6181 gccaccttgg tggtaagaag cgataatctt cttgcgaagt aacgatatcg tttttattaa
     6241 agattggcac aaataacgct aaaagaatta aaatgccaat aatcacacca gcgacaacac
     6301 ttgatttgtt tttaacaaaa cgcctcattg cgtctttaaa atatgttgtc ggtttagtgt
     6361 caaattttaa gtcatgaatc ttttcgtctt gttgaacaaa cacgaaatct ttttcagtaa
     6421 ttaattgttt cttttcactc attattttcg tgcccccatt ctaattcttg ggtcaacaat
     6481 gccgtaactt aagtccacta gtaatccggc aaaaagacca attgttgtat aaagtgccat
     6541 gtcaaccatg acaacgttat agtctttatt tgtaattgct gttacaaata agctaccaat
     6601 cccgggaatt ccgtataatg cttcaagaat cattgatcct gataaaattc caataaattg
     6661 accaataatc attggcacaa ttggcactaa actattacgt aaagcgtgac gaataacggt
     6721 ttggcgtttt gttaaacctt tcgtccttgc taaaagtaag aattcactag acataatttc
     6781 cgttaattca gcacgtgtat aacgagtaaa gccagcgata gtaccaaagg ataacgccat
     6841 tactggaatg acatatgctt tagcttgcat ttgccacgat aagccttctg gcggccattg
     6901 tgatggcaac cattctaatt tgtaagcaaa gaatagcatc aggaatgtaa taaacacgaa
     6961 agacggaatt gagataaaaa tcataactcc agtcgagata atgtggtccg gaagtttatt
     7021 tttttgtaaa gcggcaataa taccaaacaa aaatcctaac ggaaccgaaa caaagagagc
     7081 aattatattt aacttcattg acactggaag tttacgtgcg atgatttcaa tcgctggcac
     7141 attccgttca atccgtgaag atacacccca gtcccagcgt gtaatgacat tacgaagcca
     7201 acttaagtat tggcgcatca cgggcacttg gttgtaataa tataatttgc caccgtcgtt
     7261 aacagtaata tcgactgttt ggcctggcat taatggtcct tgaacttgta ccatgtagcc
     7321 aaggcgtact tgggtgttgt aatacctgag ttggtctggt gctaatccaa tcggtcgatc
     7381 catcggtaag agtttcatta aaataaacgt taagcttaaa ataatgaacg ctgtgacaaa
     7441 cacaagaccg acacgtttta gtatatattt tcccacaatt taacctcctc atatatagga
     7501 ttaaacatag aaacattgcc agcgttggct ggcaatgtcc atgtttagta attatagcaa
     7561 actaatatta gtttgtattt ttaactgata ataattgtac caattaatta ttcaccagca
     7621 ggtgcttctg gtgcaaacga tttccagagt aattcgtaaa ggttagcacg atattctggt
     7681 atacctttat aggtgtgaat ggttgaaatg tagatatcca tatagtgagc atcttctgga
     7741 acgctaatgc cttccgccac tacagtccac gcaccatttt cgaatgttgt agctgggagt
     7801 ggaacgatgc tttggaattc accctctttg ttatagaaga ctaaggctaa gaagccacca
     7861 gtttcttctt tagtaccatt cgggtcaggt gtaatgacat attcaccagc tgggtcaaca
     7921 taatatgtac cttgagcaac aaagtcatat ttggttgggt cagcttcgtt gaagccaaag
     7981 tctaaacctt tgagtgagaa tgatggaacg gttttacctt cttcaatgat ggttaagcca
     8041 agtgtacttt ctaagagtgc gtcatatgaa taacgttctc ctttgaagac gagtccacca
     8101 acttcgtatg aattgtatgt atgaccagtg tcatgacccc atgagagggt aaagccacta
     8161 cggttgtcac tacaaagagt taacatgaat gataatggat ctaaggtgtt accactgata
     8221 gttccgaagg cgaagtcaaa ttcaccaaac atcattgcat agtatgcgtc catccagttt
     8281 tgggttgcaa ataattcaat gtctaatttg aatccttcga ctgcgtcgtt aaaggcagct
     8341 tcgatcatcc gtttgagtgg tccgccttcg tcaacggttt gttggacact ttggaagtga
     8401 atacgtgggc taataacggt tgggttttca gcggttccac gtgtatattt accttccgcg
     8461 actaaatcgt tcattgcttt gttgaagagt tggccagcag cttctttgtt aaagccattt
     8521 gtttcagttg aacggccttc aaggacggct tgaccttctt cactaacacg ccatgacaca
     8581 ccactttctg ggtcaatcat gtatgcttca ctgaagaagc ccattgctgg acggctacca
     8641 gtggtatcag caagttggtc acgatttaat gaataataga caccatttaa gaagtctaag
     8701 ttggacatga ctggtttgag ttcccattcg ccacctggag cgattgagcc ttcttcacca
     8761 aataattcat tccaacgttc ttggttggca gcgttaactt gaagtttcca agtagtgtca
     8821 cctttggttt gacgtgcgcg ttcatcatta cggtattctt ttaagtaact tgcaggaaca
     8881 cccgcgctat gtaagttgtt tgctaagaat tgttggaaag cagtgttgct atcatctaat
     8941 attgtaaaga cataaccttc gtgggtatag cggtctgctt caatccaaag tgggtttttc
     9001 ttaaagacga cttgtttgtc tttttcccag atttcaaggt tgtaaacacc aagtgataag
     9061 gtgttgtcga tatcagcctt accatagttt tcaacaccga ttttgtcgat gaatgattgt
     9121 gggataggca tgaataaacc tgatgctaag ttgtacatcg catagaatgg tgatttggct
     9181 gaattgaatt caaattccat agcttgttcg gcaacattaa gtttaatacc ttgtacgcgt
     9241 tcaatgctac gttcgccaac tggtttttgt aattcttcag cgacaccaac gaatccgctg
     9301 tcttttgacg ctaagtcagt tccgcggaac catccggttt caaccatgag tttgaatggg
     9361 actaagtagt cttctaattt gacttctaag ccagcaaatt cactgtggac acctaaagtg
     9421 ttatatttta agtctccacc aactttaaga ggaactctcc acttggtagc agtaccagtg
     9481 gcttcgtcaa agtttaaagg aattggacgt tcgacgtcat ttgcgagtaa tggataccat
     9541 tcatatcctg tcttatccgc attaaatttt gtaccccaat atcctgaaga gataagagcg
     9601 tggaggtcac cagtaacgga ttcttgtgag tccatatagt taatggtacc tggatcttga
     9661 ctagtccaag tatggtagta ctttgcacgt tctggagcaa cttgtgatcc tgtaagtggt
     9721 gcaagaattt cggcttcacc aaccccgaat ccataaccaa cgacatagtc gtctgctggc
     9781 attttgaggt cgcttgagta aagcacgaca gcactgttgt cacgataagg aataccaccg
     9841 atgaatgtat caagggcgta actttcaaga agtgttaaaa tatccttacg atctttgata
     9901 gcagcaggtt tttccccagc gaatgttcta acgccactaa cggctggaac agtttcgact
     9961 aagacgtctt cgctgctaac ttctgatggt gcttctacag atgagcttgg ctttttacaa
    10021 ccgcttaatg cgactaatcc gagaagtcca acgattaaca gttttttctt tttcataaaa
    10081 ctttctcctt tcttgtagtt ttataggatt attatacata gtatatttag attgccaaga
    10141 aataggatga aaatattttc atgataacgc ttacacttgt tccaaatttg aaaaaataag
    10201 tgttttttac caaatttagt gaatatttat aaaataaaag ccccgtttaa atagggacaa
    10261 ttaatactaa ttgttggata aatcgatgaa gtcaacagtt tttatttatt atctgaatcg
    10321 atttcttcat tagtggtgtc gtcattacta gcaggcgctt tttcatcaac gactttttgt
    10381 tctgcttcca cggcagtaag tgcagcgtat tcttgttcac gaacttttaa aacttgtgta
    10441 atgtaagttt ttgtttgttc gggaagattt ggatttttaa taatttcacg taattcaaca
    10501 atggcaaaac gaagaatttg ttcgcgtttt ttctccataa ctgaatgata taaataaaag
    10561 gtaaaacgcg tagtaaatgg taaagcgata gttaacacaa ctaaaccaag taattcagct
    10621 tgtaagaaaa gcggaccatg cggattaatg gcttgtagta agaaatatcc gccaacatta
    10681 aataaagtat atagcgaaaa gacaattaaa acaatccctg tttgaacacg gcgtaaacta
    10741 cttttagttt cagcgtcacc tacaaaagta ttgcgaagca caataaagct cgttgttgga
    10801 cgatgttcaa taaatatcga taagtaaata ttaaaaacac cttttactat gataaagata
    10861 aggccgagtg ttgccacaag gttatatgca agtatttgac ttggtatata aggttcgagc
    10921 aataattcta tttttgttac taaaacattt aatgcgtctg gtgtagatgt tccttgaaac
    10981 tctaacagta agttaaacat ttgatcgtac acaggcttat caaagaaata aatgaagagt
    11041 gtcattccta atccagctat aaacattccg accaaaaagt aaataaggaa gacgacaaca
    11101 tttcgtaaat taaatatatg gcgtaatctt ccgctgcggt aagtgttttt atacaaatca
    11161 aaatacgctc gactattacg tggcgctggt gcaccagact caataagtgc tgtgtattct
    11221 gaaaatacaa caattagtgg aaagataacg aacactagca aaatcgctcc aagtagcgct
    11281 aattcggtgg aatttaaata tactgttaca aagctaaatg ctaaaaaggc taacgtaaat
    11341 aataaatagc ttttaataga acgccgttcg cgtttatagc gtttaaatac tgtacgaaat
    11401 gttaccgatt tttgcgccat tttcgtcctc tttttttgcg tagatttagt tcatcaaatt
    11461 catgaacccg cttcaacaaa tcttctggat taagcggggc aatatctcta ctctcaattt
    11521 ttaaaataag tttcataaaa tcacgagtat ctatgacaaa gtttgaacta tcattaactt
    11581 ccaaatcagt gattttcgta tctttactca cgagtgtaat agcaataaaa gtggcatgat
    11641 caagcgatgt tctaaggctt agtttttcca ctaatttttt attttttaaa agcggactat
    11701 caaccataat ttcttgtccc tttttatctt ttaaaatcca cttggaatct tgagcgtttc
    11761 cagttaaaac acccttaaat aagtaatcat taatgacata aatatactta ttaccaaaaa
    11821 gcatgtgatc aataaataaa gattcatttg aaacaagttg aatctctagt ttattaatca
    11881 aataataatc gtaatacacc gctgttttac gcactacagg ataaaaatat cgcaataatg
    11941 caaagcgttg taaaatcttt tttctgaaga aaagaaaaat attgactagt gcaaatagca
    12001 ctagcacaac cgctagcgct aatatcacat attccttaac gccaaaaaca gcaaataaca
    12061 taattattct tcagcaattt ctagggcaac acgaatcatt ttttctaaac cggtttgccg
    12121 ctcttcaaca gttgcttttt tggaatgatc aataaagcta tcagtaactg ttaaaaggca
    12181 cagtgctttc tttttaagtt tagcagcggt tgtatagagc gcatatgatt ccatttccac
    12241 acctaataca ccaaggttcg cccatttttt ccatgaattt gggtcattat cataaaatat
    12301 gtcagcgctt aaaatattgc cgacatgaac attaatgcca gaagcgtttg caacttcttt
    12361 agccttaact gctaaatcaa aatcgccaat cgcactaaaa gttccaccca aaagattgaa
    12421 ttgagcagcc caattagagt cagtacaact gccaattgcg acgataatat caaataaatc
    12481 aacttccggt tgataagtgc cgcatgttcc aacacgcatg atagtttcaa caccataata
    12541 tgtgtagagt tcatgacaat aaatgccaat ggaaggtaaa cccatcccac ttgccataac
    12601 tgatactctt tttcctttat aatatccggt ataaccaaaa atattacgaa catcagttac
    12661 tcttttaaca tcagtaagaa aagtgtttgc aatccattca gcccgctttg gatcgcctgg
    12721 cattaataca gttttagcaa aatctccctt ttttgcatta atgtgaggtg tgcccatata
    12781 attatctcct ttaattttat tttttattat agcatttatg cgctccacgt ggtacgag
//
LOCUS       DAOSLP010000031        12014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_5329_length_12014_cov_8.592274, whole genome shotgun sequence.
ACCESSION   DAOSLP010000031 DAOSLP010000000
VERSION     DAOSLP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12014
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5329_length_12014_cov_8.592274"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..811)
                     /locus_tag="PK460_03690"
     CDS             complement(242..811)
                     /locus_tag="PK460_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54215.1"
                     /translation="MILNELFKQHSILLLLFAVLVWTIFKFPKIVKRQKANTNNSRMY
                     FAKKHYNHSGKTRKQVLGKDNDLKVNELPLFNHPNKKECFVITKAMHDRIGKHKFIPL
                     YKSGDNIAGVILTSKSSYGKKKNFQIKGGYLGHELHHCIAKINLTKKDLRYSNNISYD
                     ESNYLQFLKANKKNLEKYNEFINKKQLSK"
     gene            complement(1092..1182)
                     /locus_tag="PK460_03695"
     tRNA            complement(1092..1182)
                     /locus_tag="PK460_03695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1146..1148),aa:Ser,seq:gct)
     gene            complement(1226..2659)
                     /gene="proS"
                     /locus_tag="PK460_03700"
     CDS             complement(1226..2659)
                     /gene="proS"
                     /locus_tag="PK460_03700"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOE54216.1"
                     /translation="MSKIKIDAITTQSEDFAQWYTDVCRKAELMDYSEVKGFIIYRPY
                     GYAIWENIQKYLDEKFKEKGHDNVYMPLVIPTSLFQKEKDHIEGFAPETLVATQGGNQ
                     KIEDPLIIRPTSEVLFCDHYAKIVSSYRDLPKKYNQWCSVVRWEKTTRPFLRGSEFLW
                     QEGHTIHETEVEARTHALDMLKVYENMGKELLAIPFLTGTKTEKEKFAGAEQTFTIEA
                     LMPDGKALQCGTTHYFGDGFARVFNIQFQDRNNTLQYVHQTSFGVSTRLIGSVIMAHG
                     DDNGLSLPPRLAPVQVIIVPIQQNKPGIMPKALELVSRLKAIGVRVKIDDSDKSPGWK
                     FSEHEMKGIPLRLEIGPRDLKAEVLSGSIRHSHEKISFADKDLEEVVPALLEKIHEDM
                     YQKKLTDLLNHTIEVRSIEELEDALNNKLAYAKMMWSGDQEDLDVIKEKYQATARALP
                     FNQTPFAKKCLISGKPAKYVVIIARAY"
     gene            complement(2662..4410)
                     /locus_tag="PK460_03705"
     CDS             complement(2662..4410)
                     /locus_tag="PK460_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54217.1"
                     /translation="MQDIINFILENLPRVILLSGTIVMLVIAVLLIIHGNRKDLKDQN
                     IILGNITTPRIFLLDLGEGKVTYFNRGSFGERKNGLLPQFFQQFTAKEVDRLDKWVNA
                     LIDPKSEASKFYKVDVFVRDMRRSYASILEVTGVDYEKKIIHLESYLYRYLKANDQMN
                     LTVKKKENIGYLTEEELVKVYRRAHKRGRGVYGAISIAVTASGAQTHRAEIPPHLWYE
                     LQNQVAVYRKRSRVIRFVDNHTVGIYFPRSSDVDTAIDVFQSIKKRMSGYLQKNGYNE
                     YSLGIGLTRSRQFSEMNMFFKTAEELSLYALKEDGRLLIHDPNKEIKDMDLSLYRSEL
                     TQLVARKGVDTLFQPIVNAKTVEILGYLTTFSISGSLFGSFNEAAQFARENKQNEQLV
                     KMVLRKAAARYYNMRRNNKQLLFVPLGLGGVDFISDAFSTQPFANDLKMVFMLEEQDF
                     ANTFVNIEDMLKLIKKIKKQKYEVCLIISDTELTLPDKIYGEVDYFMVNEKILTHTVN
                     DERERLSLLSSLGKLLRHQKPIMISDLPKWSEIEYYIRAGVDYVASDEISKKDNSLQT
                     IDKKKALRIINFSKRK"
     gene            4509..4601
                     /locus_tag="PK460_03710"
     tRNA            4509..4601
                     /locus_tag="PK460_03710"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4545..4547,aa:Ser,seq:cga)
     gene            4618..5145
                     /locus_tag="PK460_03715"
     CDS             4618..5145
                     /locus_tag="PK460_03715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOE54218.1"
                     /translation="MAKKRLLIVVDYQNDFVNGALGFSDAHEIEQKIIDTIEDHLKTK
                     DDIVFTKDSHFDNYLETEEGRNLPFKHCIKGTPGHDIHGKVAAYLPKAKKVYEKLTFG
                     SLELANDIVNKGYEEITLVGLVTNICILSNALLAKAALPEAKVIVLKDGVNSYDKDLH
                     AKTLDVLQGTQIIVK"
     gene            5151..5960
                     /locus_tag="PK460_03720"
     CDS             5151..5960
                     /locus_tag="PK460_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOE54219.1"
                     /translation="MNKPKDIKIIILLGQSNMEGHSWTSYLDRHFPPELVNYYTSPFK
                     TEIAYNCDNGNNESYDEFVPVRVGQGYDSTRFGPEIGIAAHLEKAQRSDEFKIIKFAI
                     GGTSLEVFWRSPSSGIPGLCYVFATNYAHRMLTKLKRDGYNPTVSAILYMQGENDSVD
                     GGHLQYEKYLQNLVNDLRNEFKDYAPKAGIYFIDGLINDAEFWTHHEIINTAKRNVAK
                     SDPKNIIVDTLTPQLSFSNEPDFEIDWAHYDSDGTIKLGHLFGEAVTEHIK"
     gene            complement(6018..6094)
                     /locus_tag="PK460_03725"
     tRNA            complement(6018..6094)
                     /locus_tag="PK460_03725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6058..6060),aa:Arg,seq:ccg)
     gene            complement(6100..6921)
                     /locus_tag="PK460_03730"
     CDS             complement(6100..6921)
                     /locus_tag="PK460_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOE54220.1"
                     /translation="MLTTLELWIIIIGMAIPFIATALGAAAVFFFRGPISERVNSILL
                     GFAAGVMIAASVWSLIMPSLEFAENSGWGKLAIIPVAVGFALGVLFLWFIDRIVPHMH
                     QRSEDEEGLPTKKLSLDSKLFLAMTIHNFPEGIAVGFAFAVAYTGIVNPGNASISLYS
                     ALALSIGMAIQNIPEGAAVSLPMARRLNNKGKAFGFGVASAIVEPIGAVLAFLFATVF
                     QTLLPWFLSFAAGAMMFVVVEDLIPEANLSGTEHQGTFAVMLGFIIMMIIDIVLG"
     gene            complement(6921..7643)
                     /locus_tag="PK460_03735"
     CDS             complement(6921..7643)
                     /locus_tag="PK460_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOE54221.1"
                     /translation="MKVENIYKQKYVLTTNYYDPSNNIGVQGTLDLFQNIAARHAYLL
                     GAGREHLEKINLVWVIARTEIEYVTYPETPLEVEVVTWPRPATRFYFDRYYKIVNPAT
                     NEIIAYSRSRWVLIDIDSRKIVGADRYDYPLDFYYEEEPFNHTFPRPSVSDNVVGNYT
                     VRPSDIDENGHLNNSKYGNIIYDYLDLQKGQKIKSIVINYNNEALQGETLTISRSRDD
                     AKVVIFGHKGDTIVFSSEVNIQ"
     gene            complement(7762..8208)
                     /locus_tag="PK460_03740"
     CDS             complement(7762..8208)
                     /locus_tag="PK460_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOE54222.1"
                     /translation="MKAKKIFWIILGCVSLALGAIGAALPILPTVPFLLLAAFSFAKS
                     SKRLHNWFINTKLYKKNLASYVKGKGMTWPAKIKMMITVTLVMLLGFIPMMIKKVYIP
                     SIILGVVWIFHIIWFIFGIKTYKKNSEDSLSTEVETPSETEEKNIE"
     gene            complement(8209..9903)
                     /locus_tag="PK460_03745"
     CDS             complement(8209..9903)
                     /locus_tag="PK460_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE54223.1"
                     /translation="MKRPNVIRRSGAKIMASLVVLLGSLSYIMVLAIITGSLGFIFAM
                     GVTLLGAVAIAKFLGEAIALSYPLIFSLLIGFGVLRGILRYIEQYTNHYIAFRLLAVL
                     RDKIFSALRTLFPSKIESKQKGSIIAMITSDIEILEVFYAHTISPIFIAIVVSISVIL
                     FVGFSSSWLLAVNALISYIVIGILLPLITSKKLKVPGVRYRQNFAAFNSFFLDSIKGI
                     KDIVLNNGGPKREVEVNERSDMLLNETKKLKKDTTRAHATTELFVSIFIFITLVLGAI
                     LVIRDNLTIGRMIIGVAAIFGSFGPVIAISSLPSNLTQTFAAGDRVLDLLNEKPLITK
                     IDNGSDFEFENVTVSNVSFAYAENEEILNDVSVHANKGEIVGIVGPSGCGKSTLLKLL
                     LRFYKKDEGTILYNGIAIENINSDNLLDNVTMVSQDTYLFDDTILENLLIAKRDATLK
                     EVEIACKKASIYDFIVSLPQGFNSRVGVFGDNISAGEKQRIGLARAFLRGSKLILLDE
                     PTSNVDSINEGIILRALKKHKEDRGIILVSHRESTMAIADRVYRFNGEIVEEVEDK"
     gene            complement(9890..11632)
                     /locus_tag="PK460_03750"
     CDS             complement(9890..11632)
                     /locus_tag="PK460_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOE54224.1"
                     /translation="MINRKLLKLIDNHKHIIFTVILMVCGLITNIVITGSLVYLIKEV
                     ANHSDDSSVWTYLWPALIAIISIIIRYLLSISVGYLTDIIGRQAKKDLRQKVYNKIVR
                     LGVRTTDGMKVAGLTQVSLEGVEQLDLYYSTYIPQFFYALLAPIILFFVTVWFDWRFS
                     LVLLAAVPLIPLSIIAISKYAKRIFAKYWGKYTAMGDKFLDSIQGLRELKIFKADAYQ
                     QTKMNEASEEFRVITMKVLVMQLASTTIMDIVAYTGAGIGVLIAIISVLKWGLSPFSA
                     LFLILVAVEFFLPLRALGSAFHVAMNGASAGQKILSLLAEEETPWGTKEVTNANIEIK
                     DVTFAYNGHANVLNDVSMTFKEQGMVSIVGESGSGKSTIVNLITGALRPQFGSVSVGG
                     ELLETLSRESYYSNLAVISYNTYIFNDTIRNNFKLANKDVTDEEIYAALTKVNLADFV
                     EANGGLDKVINEDALNISGGERQRLAVAINLVTNKAIYIFDEATNNIDIESEAIIMAN
                     IKELSKTKAVIVISHRLANVVPSDYIYYMENGELLESGNHDTLMANNSGYAKLFTTQK
                     ALEEGYMECNYEKA"
BASE COUNT         3843 a         2320 c         1865 g         3986 t
ORIGIN      
        1 tttacatgtt gttttattac aaaaaaatat tatttattct aaaacttgac atttaactaa
       61 taatatctaa taatgaagat gcaagaggaa tcttggcggt cttacccact aagtagtgag
      121 agtgatgcat tcacgtaaac ctagggccgt attaaaggga tcagtatcag catttacaat
      181 atgtatttaa accgcccctc attatttgcg ggtaagcggg ctgatcgcat cattccagca
      241 gttatttgga taactgcttt ttatttatga actcattata tttttctaag ttctttttat
      301 tcgcttttaa aaattgtaaa tagttagatt cgtcatatga aatattatta gaataacgta
      361 aatctttttt tgttaaatta atttttgcaa tacagtgatg aagttcatgt cctaaataac
      421 caccttttat ttgaaagttt tttttcttgc cataagaact tttacttgta agtattactc
      481 cagcaatatt atctccgctt ttatataatg gtataaattt atgttttcca attctatcat
      541 gcattgcttt agttattaca aaacactctt tcttatttgg gtgattgaat aatggtagct
      601 cattaacttt taaatcatta tccttaccta atacctgttt tctagttttc ccactatgat
      661 tataatgttt ttttgcgaag tacattctac tattatttgt attagctttt tggcgcttta
      721 caattttggg aaacttgaaa atggtccata ctaaaacagc aaataacaaa agtaatattg
      781 agtgttgttt gaataattcg tttaatatca tctttaactc ctatttatta cccttaatgt
      841 tttttttgtt ttccttcaat ttcgcatttt tcgattattc aatattgttt aatctctttt
      901 catattgttt tttgaagaaa ttcagtgaaa tcttatccaa tatataataa taataataat
      961 aaagacgcta taattcttaa tttttatttt tgttaaaatg tttatttaaa acatatttat
     1021 cacatttttg tttgtggttt aaattcaaaa taaagaaaag cagattttca tcaaatctgc
     1081 tgattaacta atggcggagt aggtgagact cgaactcacg cgccagttac ccgacctact
     1141 tccttagcag ggaagcccct tcaccaactt gggtactact ccgtcttgct aaataatatc
     1201 acatgcttaa tattttgtaa attaattaat aggcacgagc gatgattaca acatatttag
     1261 cgggtttccc actgattaaa cactttttag caaatggagt ttgattaaaa ggtaaggctc
     1321 tagcggttgc ttgatatttt tctttaatca catctaagtc ttcttgatca ccactccaca
     1381 tcatcttagc gtacgctaat ttgttgttaa gggcatcttc aagttcttca atacttctta
     1441 cttcaatggt gtgattaagt aaatcagtaa gtttcttttg atacatatct tcatgaatct
     1501 tttctaagag tgccggcact acttcttcta aatctttatc ggcaaaacta attttttcat
     1561 ggctatgacg aattgaacca cttaaaactt ccgcctttag gtcgcgcgga ccaatttcta
     1621 agcgcagcgg gatacctttc atttcatgtt cactaaattt ccagcctggt gacttatcgg
     1681 agtcatcaat tttaactcta acaccaattg cttttaagcg actgacaagt tctaaggctt
     1741 ttggcataat tcctggttta ttttgttgga tagggacaat gataacttga acaggagcaa
     1801 gccgtggtgg taggctaagc ccattatcat cgccatgcgc cataataaca ctgccgatta
     1861 aacgcgttga aacaccaaaa ctagtttgat gcacatattg taaagtgtta ttacgatctt
     1921 ggaattgaat attaaaaaca cgggcaaaac catcaccaaa gtagtgagtt gtaccacatt
     1981 gtaaagcttt accatctggc ataagtgctt caattgtaaa agtttgttca gcgccggcaa
     2041 atttttcttt ctcggtttta gtaccggtta aaaacggaat cgcgagtaat tctttaccca
     2101 tgttttcata aactttaagc atatcaagcg catgggtccg tgcttctact tccgtttcat
     2161 gaatcgtgtg cccttcttgc cataagaatt cactaccgcg taaaaaagga cgcgttgtct
     2221 tctcccagcg aacgacagaa caccactgat tatatttctt tggtaaatca cgatatgagc
     2281 taactatttt agcgtagtga tcacaaaata atacttcact tgtcggacga ataataagtg
     2341 gatcttcaat cttttgatta ccaccttgtg ttgcgactaa tgtttcgggg gcaaaacctt
     2401 cgatatgatc tttttctttt tgaaaaagac ttgtgggaat aacaaggggc atgtacacat
     2461 tatcatgacc tttttcttta aacttttcat ctaaatattt ttgaatattt tcccaaatcg
     2521 cataaccata aggtcggtaa ataataaaac ctttaacttc gctataatcc attaattccg
     2581 ccttgcgaca cacatcagtg taccattgag caaaatcttc actttgggtt gtaattgcat
     2641 caattttaat cttactcatc tttatttcct cttactaaaa ttaataattc ttagtgcttt
     2701 ctttttatca atagtttgca agctgttgtc tttcttgcta atttcatcac tagcgacata
     2761 atctacacct gcccgaatat aatactcgat ttcactccac tttggcaaat cactaatcat
     2821 tattggtttt tgatgacgta ataatttacc taatgatgaa agtaaggata agcgttcccg
     2881 ttcatcatta actgtatggg ttaagatttt ttcattcacc ataaagtaat caacttcacc
     2941 ataaattttg tctggcagtg ttaattcagt atcactaatg attaaacaca cttcatactt
     3001 ttgtttttta attttcttaa tcagttttaa catatcttca atgttcacaa atgtgttagc
     3061 gaaatcttgt tcttctaaca taaagaccat tttgagatca ttcgcaaatg gctgcgtact
     3121 aaaagcatca ctaataaagt cgactccacc taacccaagg gggacaaata gtaattgctt
     3181 attattgcgg cgcatattgt aataacgggc cgctgccttc cggagcacca tctttacaag
     3241 ctgttcgttt tgcttatttt ctcgcgcaaa ttgcgcagct tcattgaaac tcccgaataa
     3301 tgaaccgctg atgctgaagg tcgttaagta ccctaatatt tcaactgttt tagcattaac
     3361 aattggttga aataaagtat cgactccctt gcgagccaca agttgtgtta gttcactgcg
     3421 atataaactt aagtccatgt ctttaatttc tttatttgga tcatgaatta agagccgacc
     3481 gtcttcttta agcgcataaa gacttaattc ttccgctgtt ttaaaaaaca tattcatctc
     3541 cgaaaattga cgagaccgcg ttaacccaat cccgagtgaa tattcgttgt agccattttt
     3601 ttggagataa ccgctcatgc gtttctttat tgattggaac acatcaatag cggtatcaac
     3661 atcacttgaa cgcgggaagt atatgccaac agtatgatta tcaacaaaac gaatgacgcg
     3721 acttcgtttc cgataaacag cgacttggtt ttgtaactca taccataaat gcggcggtat
     3781 ttctgcgcgg tgcgtttgtg ctccgcttgc tgtcactgca atactaatcg caccatatac
     3841 gccacgcccg cgcttatggg cgcgtctgta tacctttact aattcttctt cagttaagta
     3901 acctatattc tcttttttct tcaccgttaa attcatttgg tcattagcct ttaaatatcg
     3961 atacaaataa gattcaaggt gaataatctt tttttcataa tcgacaccag ttacttctaa
     4021 aattgaagcg tatgaccgac gcatatctcg cacaaaaaca tcaactttat aaaatttact
     4081 tgcttcactt tttggatcaa ttaaagcgtt aacccattta tcaagtcgat caacttcttt
     4141 cgcggtaaat tgttgaaaaa attgtggtaa taaaccattt ttgcgttcgc caaaactacc
     4201 gcgattaaag taagtgactt ttccttcacc taagtcaaga agaaaaatcc gcggtgttgt
     4261 tatatttcct aaaataatgt tttggtcttt aagatcttta cgattaccgt gaataattaa
     4321 aagcacagca ataactaaca tcactattgt gccgctaagt agtattaccc ttggtaagtt
     4381 ttctaatatg aaattaataa tgtcctgcat aaatcaccta tgtatattat acataaaaat
     4441 aattttatca ttgcgtagaa gcgaatattt tatagatact ataaaatctt tgttataatt
     4501 agcattacgg agaaataccc aagcctggct gaaggggtcg gtctcgaaaa ccgataggag
     4561 gagcaatctt cgcgagggtt cgaatccctc tttctccgcc atgaaggagg aaaacaaatg
     4621 gcgaaaaaac gtttattaat tgttgtggac tatcaaaatg acttcgttaa tggagcactt
     4681 ggctttagtg atgcccatga aatcgaacaa aaaatcattg atactattga agaccactta
     4741 aaaacaaaag atgacattgt cttcactaaa gacagccatt ttgataacta tttagaaact
     4801 gaagaagggc gtaatttacc tttcaaacac tgtatcaaag gtacaccggg tcatgatatt
     4861 catggtaaag tagcagccta cttaccaaaa gcaaagaaag tttatgaaaa gttaactttt
     4921 ggttcacttg aactcgctaa cgatattgta aataaaggtt atgaagaaat tacgcttgtt
     4981 ggtttagtga caaatatttg tattttaagt aatgctttac ttgctaaagc cgctttacct
     5041 gaagcaaaag ttatcgtcct taaagacggt gtcaatagct atgacaaaga cttacacgct
     5101 aagacactag atgtgttaca aggcacacaa attatcgtta aataaacttt atgaacaaac
     5161 ctaaagatat taaaatcatc attcttttag gccaatcaaa tatggaaggc cattcgtgga
     5221 ctagttattt agaccggcat ttcccgcctg aattagttaa ttactacact tcccctttca
     5281 aaacggaaat tgcctataac tgtgataatg gtaataatga atcatatgat gaatttgtcc
     5341 ctgtcagagt tggacaagga tatgactcta cacgttttgg tcctgaaatt gggattgctg
     5401 cgcacttaga aaaagctcag cgcagtgatg aatttaaaat tattaagttc gcgattggtg
     5461 gcactagcct tgaagtattt tggcgctcac catcaagtgg gatccctgga ctatgttatg
     5521 tgtttgccac taattatgcg caccgtatgc ttacaaaatt aaagcgtgat ggctataatc
     5581 caactgtcag cgctattctt tatatgcaag gtgaaaatga ttcagttgat ggtgggcatc
     5641 ttcaatacga aaagtattta caaaatcttg ttaatgattt acggaacgaa tttaaagatt
     5701 acgcacctaa agcaggcatt tactttattg acggccttat taatgatgct gaattttgga
     5761 cacatcacga aataattaat accgctaagc gtaatgtagc aaaaagtgat ccaaaaaaca
     5821 tcattgtcga tacacttacc ccacaacttt cttttagcaa tgaaccggat tttgaaatag
     5881 actgggcaca ttatgatagt gatggtacta ttaaacttgg tcacttattt ggtgaagctg
     5941 ttactgaaca tattaaatag ttactaataa caataaacaa aaaagcaccc gcgactgggt
     6001 gcttttctct ttctttatgg cgcgcccgac acgactcgaa cgtgtgaccc caaccttcgg
     6061 agggttgtac tctatccacc tgagctacga gcgcatttat taccctaata ctatatctat
     6121 aatcatcata ataataaagc ctaacattac agcaaatgtg ccttggtgct cagttccact
     6181 taaatttgct tcaggaatta aatcttccac aacaacaaac atcatcgctc ctgcggcaaa
     6241 acttaaaaac cacggtaaga gtgtttgaaa caccgtggca aatagaaacg ccaaaactgc
     6301 accaattggt tcaacaattg cactagcaac accaaatccg aatgctttac ctttattatt
     6361 aagacgccgc gccatcggta aactaacagc cgctccttca ggaatatttt gaattgccat
     6421 cccaattgag agggctaggg cactatagag cgaaatagaa gcatttccag gattaacaat
     6481 acctgtataa gccacagcaa aagcaaagcc gaccgcaata ccttcaggaa agttatgaat
     6541 tgtcattgct aaaaataatt ttgaatcaag acttagtttt ttagttggta atccttcttc
     6601 atcttcacta cgttgatgca tatgagggac aatacggtca ataaaccata aaaagagtac
     6661 tcctaatgca aatccgaccg caactggaat aatcgctaat ttaccccagc ctgaattttc
     6721 agcaaattct aaacttggca ttattaatga ccacactgaa gcagcaatca ttacacccgc
     6781 agcaaatcct agtaaaatac tattaactcg ctcacttatg ggcccgcgga aaaagaatac
     6841 cgctgctgca ccaagcgcag tcgcaatgaa tggtatagcc atcccgataa taataatcca
     6901 tagttcaagt gttgttaaca ttactgaata ttgacctcgc tactgaacac aattgtatca
     6961 cctttatgcc caaaaattac gacttttgca tcgtccctgc tgcgactaat tgttaaggtt
     7021 tcaccttgta aagcttcatt attataatta attacaatac ttttaatctt ttgacctttt
     7081 tgtaaatcaa gataatcata gattatattt ccgtattttg aattatttaa gtgtccgttt
     7141 tcatcaatat cacttgggcg tacggtgtaa ttacctacaa cattatcact aacactgggt
     7201 cgcgggaagg tgtgattgaa aggttcttct tcataataga aatcaagcgg ataatcataa
     7261 cgatcagcgc ctactatttt acgcgaatca atatcaatta aaacccatct acttctggag
     7321 taagcaatta tttcattcgt tgctgggttt acaattttat aatagcgatc aaaataaaac
     7381 cttgttgcag gacgtggcca agtcacaacc tcaacttcta gcggagtttc aggataagta
     7441 acatactcta tttctgtgcg cgctataacc cacactaagt taattttttc taagtgttca
     7501 cgaccggcac ctaataaata agcgtgacgg gcggcaatat tttggaataa atcaagtgtg
     7561 ccttgtacac caatattatt acttggatca taataattag tggtgagcac atatttttgt
     7621 ttataaatat tttctacttt catcgtcgct cttcctcctt taatctttag attaaggcaa
     7681 cattataata ttacaccatt tgtgtgaaaa taaaggctat ttatactatt ttatttatat
     7741 tttttactta ctttttaaat tttattcaat gtttttttct tcggtttcgc ttggtgtttc
     7801 tacttcggtt gaaagcgagt cttcgctatt tttcttataa gttttaatac caaaaataaa
     7861 ccaaatgata tgaaaaatcc acacgactcc taaaataatg ctaggaatat atactttttt
     7921 aatcatcatc gggataaatc caagtaacat cacaagtgtc actgtaatca tcatctttat
     7981 ttttgccggc caagtcatgc ctttgccctt aacataactt gctagattct ttttatatag
     8041 ttttgtatta ataaaccaat tatgtaaacg tttcgaactt ttggcaaaac taaaagcggc
     8101 gagcaaaaga aatggaaccg tcggtaaaat aggaagtgct gctccgattg caccaagtgc
     8161 gagactgaca caacctaaaa taatccaaaa tatctttttt gctttcattt atttatcctc
     8221 aacttcctct actatttcac cattaaaacg atagacacga tcagcgattg ccattgttga
     8281 ctcacggtgt gatacaagaa tgatgccgcg atcttcttta tgttttttta atgctcttag
     8341 gataatgcct tcgtttatac tatcaacatt actagttggc tcatcaagta atattaattt
     8401 gctaccacgc aagaacgcac gagctagacc aatacgctgt ttttcaccag cggatatatt
     8461 atcgccaaac actcctacac gtgaattaaa accttgcgga agtgaaacaa taaaatcgta
     8521 aattgatgcc tttttgcaag caatttccac ctcttttaat gttgcatcac gtttagcgat
     8581 taataaattc tcaagaatag tgtcgtcaaa aagataagtg tcttggctga ccattgttac
     8641 attatctaaa aggttatcac tgttaatatt ttctattgca ataccgttat ataaaatcgt
     8701 accttcatct tttttgtaaa atcttaataa caattttagt aatgttgatt tgccgcatcc
     8761 cgaaggacca acaattccaa ctatctcacc cttattagcg tgtacactaa catcattaag
     8821 tatttcttca ttttcagcat aggcaaaaga cacattacta actgttacat tttcaaattc
     8881 aaaatcactt ccgttatcaa tcttagtaat cagtggtttt tcatttaata aatcaagtac
     8941 acgatcgcct gctgcaaatg tttgcgttaa attacttggt aaagaagaaa tagcgataac
     9001 aggaccgaat gaaccgaaga tagcagcaac gccaataatc atacgcccaa tagttaaatt
     9061 atcacgaata acaagtatcg ccccgagcac taatgtaata aagataaaga tggaaacaaa
     9121 tagttcagta gtagcgtgtg ccctagtcgt atcttttttc aacttttttg tttcgttaag
     9181 caacatatca gaacgttcat taacttctac ttcacgtttt ggtccaccat tgtttaaaac
     9241 aatatcttta attcctttaa tactatcaag gaaaaatgag ttaaaggcgg caaagttttg
     9301 gcgataacga acacccggaa cttttaattt ctttgatgta ataagtggta gtaaaatacc
     9361 gatgacaata taactaatta gggcatttac cgctaataac caacttgaag aaaaaccaac
     9421 aaataaaata acactaatcg acacaactat agcaataaaa ataggggaga ttgtatgagc
     9481 ataaaacacc tcgagtattt caatatccga agttatcatc gcaataattg atcctttttg
     9541 tttactctct atctttgaag gaaataatgt tcgtaaagca ctaaatatct tatcgcgtag
     9601 caccgctaaa agccgaaaag caatataatg atttgtgtat tgttctatgt atctaaggat
     9661 tccgcgtaaa acaccaaatc caattaacaa actgaaaatt agcggatatg aaagtgcaat
     9721 tgcttcacct aaaaacttag ctatcgctac tgctcctaaa agtgtgacac ccatcgcgaa
     9781 aataaatcct aatgagccag taataattgc taaaaccata atataagata agctacctaa
     9841 aagtacgaca agacttgcca taatttttgc cccacttctt cgtattacat taggcctttt
     9901 cataattaca ctccatgtaa ccttcttcta atgctttttg ggtcgtaaat aatttagcgt
     9961 agccactatt attcgccatt aaagtatcat ggttaccact ttctaataat tcaccatttt
    10021 ccatataata aatataatca ctaggcacta cattagcgag acgatgagaa ataacaatta
    10081 ccgctttcgt tttcgataat tctttaatat tagccataat tatggcttcg ctttctatat
    10141 caatattgtt agttgcttca tcaaatatat atatcgcttt gttagtgact aaattaattg
    10201 ccacagcaag gcgttgcctt tctcctcccg atatatttag cgcatcttca ttaataactt
    10261 tatctaaccc accatttgct tcaacaaaat cagcaagatt aacttttgtt aaagccgcat
    10321 atatctcttc gtcggttaca tctttatttg ctaatttaaa attattgcgg attgtatcat
    10381 taaaaatata agtattgtaa ctaatgaccg caagattact ataataactt tcacgcgaca
    10441 gagtttctaa taattcgcca ccaacactca cactcccaaa ttgcggtcta agtgcacctg
    10501 taataagatt aacaattgtt gattttccac ttcctgattc accaacaatc gaaaccatgc
    10561 cttgctcttt aaaagtcatt gatacatcat ttaacacgtt agcgtgaccg ttatacgcaa
    10621 aagttacatc tttaatttcg atatttgcat tcgttacttc ttttgtaccc catggcgttt
    10681 cttcttctgc aagcagtgat aatatctttt gaccagcact tgccccgttc attgcaacgt
    10741 gaaacgccga gccaagcgca cgaagcggta agaaaaactc gactgcgact aaaattaaaa
    10801 ataacgcact aaatggtgat aatccccatt ttaaaacact aattattgct attaatacac
    10861 caattccagc gccagtataa gcgacaatat ccataatcgt tgtactcgct aattgcatga
    10921 ctaacacttt catagtaatc acacgaaatt cttcacttgc ttcattcatc tttgtttgct
    10981 gataagcatc ggccttaaag attttaagtt cgcgtaatcc ttggatacta tcgagaaatt
    11041 tatcacccat tgctgtgtat tttccccagt atttagcaaa gatccgtttt gcgtatttag
    11101 atatagcgat aatggaaagt ggaattaggg gtaccgctgc tagtagcacg agtgaaaacc
    11161 gccaatcaaa ccataccgtt acaaaaaaca aaattattgg cgctaatagt gcgtagaaaa
    11221 attgtggaat ataagttgag taatataaat ctaattgctc cacgccttca agtgaaactt
    11281 gcgttaaacc agccaccttc atcccgtcag tagttctcac gccaagtctt acaatcttgt
    11341 tatacacttt ttgacgcagg tctttttttg cttgacgacc aataatatct gtcaagtaac
    11401 caacactaat tgaaagtaaa taacgaataa taattgaaat aatagcaatt aatgcaggcc
    11461 ataaataagt ccatacacta ctatcatcac tatgattagc gacttctttt attaaataaa
    11521 caagcgatcc agttattaca atatttgtaa ttaatccgca taccatgagt atcacagtga
    11581 aaataatatg tttgtgatta tcaattaatt tcagtaactt tcgattaatc atttatgctt
    11641 tataactcct aattacttaa gcttattagt tagcgaacgc taacctttat ggtaaaaaag
    11701 aaggcggcga tattcttgtt agtcgccgct aacttcgtac taactataca ttccatcctg
    11761 tcaattgtca atgataacta taatattcat ccttgagttt tgtctggtaa ttacaataaa
    11821 agtaatgata cgagcattat aaaaagatac tattattaag tcactaatgt agaatacact
    11881 actttattag aattattgaa gttcacatca ttgatttttc agtagtgtta tgttattttg
    11941 aatgtagaga atttatattt aaggagatta actattctta atcaagttaa atagtaaata
    12001 tatatcaaaa agat
//
LOCUS       DAOSLP010000032        11744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_5452_length_11744_cov_8.092908, whole genome shotgun sequence.
ACCESSION   DAOSLP010000032 DAOSLP010000000
VERSION     DAOSLP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5452_length_11744_cov_8.092908"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PK460_03755"
     CDS             <1..519
                     /locus_tag="PK460_03755"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOE54225.1"
                     /translation="EAVDKLRDTSSSHHRCSIIEVMGNRCGDLALYSGTACGVDLIIS
                     PEYRPSEEEIIETLKNINLNRARHALVIVSEKQFEDLEKLRERIVEETGFDAKIEVLG
                     RIQRGGSPTASDRVLAGRLGVYAIDLIAKGIDNVCVGLVDGRLRHHAIAEVTKSTNPN
                     LDELLNVIDKLK"
     gene            534..1979
                     /gene="pyk"
                     /locus_tag="PK460_03760"
     CDS             534..1979
                     /gene="pyk"
                     /locus_tag="PK460_03760"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOE54226.1"
                     /translation="MLNISKKTKIVATIGPVTNTLEKVRELIDAGMNVMRINFSHGTY
                     ETHKTIIDISKKLIEEHIYIPIMLDTAGPEIRCHFFEDGKVEIVKDSIVTINMKEVLG
                     TATEFSVNYPKLVDDILVGDMIKIDDGKLRLDVIEVDREKRLIKVKAFNTHVISNRKG
                     INIPGERMTLPALSERDKKDIKFGIENGIDMIAASFTRCAADVIQLKNYLAELGAPEI
                     PIIAKIENKQGVDNIKEILEVADGIMVARGDMGVDIPPEVVPVMQSDIILECRKVGKP
                     VIVATQMLDSMQTNPLPTRAEVSDVATAIKEGADAVMLSAETASGLYPVESAAMQQRI
                     AITMEEFLPYTQLANFGYNYSKKKRSDAIATSVASTANLIDAKAIVCFSETGKSAICI
                     SKSRPKCPIIMVTSSPAAGMRVAPYFGIYSIVVKTLPQLIEDMEAFALIKMRQLGLKP
                     NDNIIITGGTPTGTGSTNFLRIVALNKLGDI"
     gene            1979..2821
                     /locus_tag="PK460_03765"
     CDS             1979..2821
                     /locus_tag="PK460_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOE54227.1"
                     /translation="MFIIALDIGGTNIRAAIADQNGKLLEVVKEKTLRHDKEIFITQV
                     KGIITQLDLSKYDPIAISIGVPGRVRQNGFIDELPNIGITNIDLVSALQKTFKLPVSV
                     RNDAEMAAFAEAALGAGKELKSTYFVTISTGIGGCLIVDRTIKNPSPEIGHTLVPYRG
                     NFYELEKIASGDGVINLAALNEIYYANAQAFFKDVTYKVPYALKIFDEWLDLITDFFI
                     FVNEAFTPDVIAVTGGVLKSKKLFWDKFIERVPNANIQIAHFSEDAGLVGAASYGFKV
                     FASQ"
     gene            3085..4983
                     /gene="thrS"
                     /locus_tag="PK460_03770"
     CDS             3085..4983
                     /gene="thrS"
                     /locus_tag="PK460_03770"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOE54228.1"
                     /translation="MKELKYNNQLVKVNDGTTGFDLLKILNIEGRSNVLVYKLNGELF
                     DLATPVEQDGEFSFVTKEDEEAFAILNHDAAHLMAQAILSIYPGAKFGIGPDISEGFY
                     YDVDFGDVVINDGDLAVIEAKMMELSRADHRIERQVVSKEKALEIFAKNEYKVELINE
                     IEERITIYSQGDFVDLCYGPHMPSTRYVQHFKLLSLAGAYWRGDASRKQLTRIYGVAF
                     FSKDALDKHLYNLEEAKKRDHRKLGRELGLFMLSEYGPGFPFWLQKGMTLYHELEHWW
                     IKKQREAGYEFVKTPQILSRELWETSGHWLNYRENMYTTLIDERDFAIKPMNCPGSIL
                     IYNNGLHSYKDLPIRMAEHGHVHRHEPSGSLNGLFRVRSFTQDDAHIYCRKDQLQSEI
                     KRLLKLYDEVYKTFNLSYHIELSTRPEKKYIGDLKTWNLSEKALAKACIASGIPFKIN
                     EGDGAFYGPKLDFKLRDSLGRIWQCGTIQLDMNLPERFDISYIDANNQKVRPIMLHRA
                     VFGSIERFIGILIEHYAGAFPLWLSPTQIAVLPVNNDAHLKHAKSVVRKLEKQGLRVE
                     LDDSNEKLGYRLRNSQINKIPYTLVIGDKERNDNLVTYRKFGDKAQITVPYREFITLI
                     KREIRDKA"
     gene            5039..5650
                     /locus_tag="PK460_03775"
     CDS             5039..5650
                     /locus_tag="PK460_03775"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOE54229.1"
                     /translation="MAFTLKPLPYAYDALEPYIDAKTMEVHHSKHHATYVANLNAALA
                     KDPSFNDWSLEKILKNLDKLPESIRTAVRNNGGGVYNHDLFWTLLKVNNGKGPEGKLK
                     DALVATFGTFEAFKDAFATAAKSRFGSGWAWLVVTSDKKLKVVSTPNQDIPFDEGTPI
                     LTLDVWEHAYYLKYQNRRPEYVEAFFNVINWDEVARLYENALK"
     misc_feature    5702..5814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            5823..6374
                     /gene="infC"
                     /locus_tag="PK460_03780"
     CDS             5823..6374
                     /gene="infC"
                     /locus_tag="PK460_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOE54230.1"
                     /translation="MLIYNQNRERPERNTDLVNENIRFPEVLVIGPNGEQLGVMKTRA
                     AIDLAASEYELDLYCVNPNGKPPVCKMLNYSKHRFEQQKRAKEQKRKQKTVELKEIRM
                     SPVIDVHDQETKGRMARRFLEDGNKVKVTIRYRGRQMAHIDVGEKILESFVASLEDYA
                     IVERKPLMEGRNLSVILASKIKK"
     gene            6386..6580
                     /gene="rpmI"
                     /locus_tag="PK460_03785"
     CDS             6386..6580
                     /gene="rpmI"
                     /locus_tag="PK460_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOE54231.1"
                     /translation="MPKMKSKSALTKRIKVTKSGKLMRKAAYTSHLAPRKTTKQKRQL
                     RKARLVHKSDAKRIKYLIQN"
     gene            6593..6952
                     /gene="rplT"
                     /locus_tag="PK460_03790"
     CDS             6593..6952
                     /gene="rplT"
                     /locus_tag="PK460_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOE54232.1"
                     /translation="MARVKSSPVTRARRKKVLKQAKGYFGAKSLLFRTAKEQVMHSLR
                     YAYIDRRRIKRDYRKLWIKRINAGCRMCEISYSQFMHGLKLANVDINRKMLSELAIND
                     FETFKGLTETAKKALAK"
     gene            complement(7030..7356)
                     /locus_tag="PK460_03795"
     CDS             complement(7030..7356)
                     /locus_tag="PK460_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54233.1"
                     /translation="MNQSEIMKNLKQEIKSSYEGFLKLGIHDEQAFHEHKEAIAFSVH
                     TVKAIAKILEPIPNEILYFKGGIGDTLAAVKEYAKNLVSKFGTLNTYKLYDFMSIDLP
                     TLRDTL"
     gene            complement(7357..7623)
                     /locus_tag="PK460_03800"
     CDS             complement(7357..7623)
                     /locus_tag="PK460_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54234.1"
                     /translation="MGRLTTKLPNILRVFDMGDALAFKEEEHYYIIIKRRSHLPIYNW
                     RYFELVIRSVCEIKNMTLISALEIEDNPFKAYFIEKGTLIKRAI"
     gene            complement(7636..8808)
                     /gene="thiI"
                     /locus_tag="PK460_03805"
     CDS             complement(7636..8808)
                     /gene="thiI"
                     /locus_tag="PK460_03805"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOE54235.1"
                     /translation="MLMIHFGELYTKGKNRRDFIRQLTNNIKEVVANFKVQIETRHDH
                     IYIKNIKEKDVAEITKRLQNVSGIHAISEVIEFPLDLEKVKQFALELILELKPKTFKV
                     ITKRKDKLFPLTSDEINRSVASHILKNAEIKVDVHNPELPLRLEIDKSRILVVGKRYD
                     GLGGYPLGAAGKGLVLLSGGIDSPVAAYLMIRRGIKIAAIHFAAPPYTSEEVLKKIEA
                     LTAKLNNYQPHIRLYVVPFTKLQLAIYEHATSGYAITLMRRTMLRISTVIAKRHGYKV
                     LVTGDSLGQVSSQTLESLVVINEVTNYPIIRPLATLDKNEIIKIAKDIETYPISILPY
                     EDCCTIFPVTNSVTSPKLEDAVLFESKFDYESLISEAIRDTKKVDIRLDDGKEDLF"
     gene            complement(8810..9943)
                     /locus_tag="PK460_03810"
     CDS             complement(8810..9943)
                     /locus_tag="PK460_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOE54236.1"
                     /translation="MIYFDNASTTKIHPEVLRTYIEILEKYYGNPSGMHTFAREIARL
                     QDKSREEILRMFKFRDGKVIFTSGATEANNIALIGLYEAYKNRGNEIIISDYEHPSVR
                     AACEYLEKERGAIIHKVPIRFKGGFDFDVFKSLLNDKTVLVSVMAVNNEVGDVIDLGE
                     IRACLKKYPKVIFHTDVTQALGKLDFHPNAADAYSFSAHKIGGLKGSGALLLRKNLKP
                     KPLVYGGSQEEGIRPGTHNAPANIVLAKTVRIAEGNMDKFYDHSYRLALKLHNFLATH
                     KDLFIINSVGGSNPYIVNFSLKTIKASIVLNALESRDIYVGTTSSCSAKLHAMSKSVY
                     AISQDEERASNSIRVSFGMENTLDEVEIFCNTLTEIIKEYGRE"
     gene            complement(9940..11655)
                     /locus_tag="PK460_03815"
     CDS             complement(9940..11655)
                     /locus_tag="PK460_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005940 - septin ring [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HOE54237.1"
                     /translation="MKVVTLLNTLDIVFIVASIILVLAVFGFILSKFAFRGTKNKKAL
                     KSLSIKYSYVRSLLLGENIQYIKRIEAISDINLLMRPAYEEFFTRFEQLRNVDDRKAS
                     RSIQILNEALAAGNKEFKKAFNEHRPIILDYERKVTELSKDLTNMIKPESEIREESVY
                     DKEKLRIIKNIYRDHKDELEICVPSFEKIFTQIELLFKEYDNALNGAYYDEARSYLEK
                     IRTFNDQLERILQILPLLVVKATVLIPEKIATLKKSYEEATAYNVPLHHLRVSAEIES
                     FNEQLEVIKEQLIRFDTRNVEQTLNQIADTIDELINSFNVEIEKQREFTAEYDKTYLH
                     VNELEQRYIRLVNRMPQVKEYYLINPEYEKKLEEANTNVNTMMMVKRSLDAFLHSATK
                     QPYSVLVEKVQQLQAESMKVEVLVREFQTYIESLRVIVEDGFAICKLLFIRFKELEVT
                     LRKLNVPAYIQNFEQDFDSGYAMISDISTIIRERPIDVSQVEVLTNELRALATRLEDV
                     ITTDVKYAHSAEEVIVSLNAFRPQFSELHSKLRDEEQRFFNGLFKHTYENSIEQLKKY
                     QNRKQ"
BASE COUNT         3778 a         2103 c         2164 g         3699 t
ORIGIN      
        1 gaagctgttg acaaacttcg tgatacttct agcagccatc accgctgttc aattattgaa
       61 gttatgggta atcgctgtgg cgacttagcg ctttatagcg gaacggcatg tggggttgat
      121 ttaattattt cccccgaata tagacctagc gaagaagaaa ttattgaaac actaaaaaac
      181 attaacttaa atcgcgcacg acacgcgctt gtgatcgtta gtgaaaaaca atttgaagat
      241 ttagaaaaat tgcgggagcg tatcgttgaa gaaacaggct ttgacgctaa aattgaagtg
      301 ttagggcgca ttcaacgcgg cggctcaccg acggctagtg atcgggtact tgccggtcga
      361 cttggtgtat atgcgattga tttaattgct aagggtattg acaatgtttg tgtcggctta
      421 gttgatgggc gcttacgaca tcacgccatc gctgaagtta ctaaatcaac taatccaaat
      481 ttagatgaat tactaaatgt aattgataaa ctcaaataga aaggaacacc tttatgttaa
      541 atatttccaa gaaaactaaa attgtcgcca caattggacc agtgacgaat actcttgaaa
      601 aagttcgaga attaattgat gccgggatga atgttatgcg catcaacttc tcacacggca
      661 cttatgaaac acacaaaaca attattgata tttccaaaaa actaatcgaa gaacatattt
      721 atattccgat tatgcttgat acagctggac cagaaattcg ttgtcacttc tttgaagatg
      781 ggaaagttga aattgtcaaa gactccatcg tcaccatcaa tatgaaagaa gtattaggca
      841 ccgctacaga atttagtgtt aattatccta aattagtcga tgatattctc gtcggtgaca
      901 tgattaagat tgatgatggc aagttaagac ttgatgttat tgaagtcgat agagaaaagc
      961 gtttaatcaa agttaaagca tttaatacgc atgttatttc caatcgtaaa ggcattaata
     1021 ttcctggtga acgaatgact ttaccagcac taagtgaacg cgataaaaaa gatattaaat
     1081 ttggaattga aaacggcatt gacatgattg cggcaagttt tactaggtgt gctgctgatg
     1141 ttattcaact taaaaattat ttagcggaac taggtgcacc ggaaattccg attatcgcta
     1201 aaattgaaaa caaacaaggt gttgataata ttaaagaaat cttagaagtc gctgatggga
     1261 tcatggttgc ccgtggtgat atgggagttg acattccacc tgaagtcgtt cctgtgatgc
     1321 aaagcgatat cattttagaa tgtcgtaagg tcggcaaacc agttatcgtt gctacacaaa
     1381 tgcttgactc aatgcaaact aatccgctcc caacaagggc ggaagtaagt gacgtcgcga
     1441 ctgccattaa agaaggcgct gacgctgtga tgcttagtgc ggaaacggct agcggtttat
     1501 atccggttga aagtgcagca atgcaacaac ggattgccat tactatggaa gaatttttac
     1561 catatactca acttgcaaac tttggctata actattcaaa gaaaaagcgc agtgacgcga
     1621 ttgccacaag cgtcgcctca acggcgaatt taatcgacgc taaagcgatt gtttgtttta
     1681 gtgaaacggg taaaagtgcg atttgcatat ctaaatcacg tcctaagtgt ccgatcatta
     1741 tggtcacgag tagtcccgcg gcgggaatgc gagttgcacc ttactttggc atttatagta
     1801 tcgtggtaaa aacactacca caattaattg aagatatgga agcttttgcg ctcattaaaa
     1861 tgcgacaact tgggttaaaa ccaaatgata atatcatcat tactggtggt acgccaactg
     1921 gcactggttc gacaaacttc ttacggattg tcgcccttaa taaattagga gatatttaat
     1981 gtttattatt gcacttgata ttggtggcac aaatattcgc gcagcaatcg ctgaccaaaa
     2041 cgggaaatta cttgaggttg taaaagaaaa aacactgcgc catgataaag aaatttttat
     2101 cacgcaagtt aaggggatta ttacacaact tgatttaagt aaatatgatc ctattgctat
     2161 ttccattggt gtaccgggtc gcgttagaca aaacggattt attgatgaat taccaaatat
     2221 tggcattact aatatcgatt tagttagtgc tttgcaaaag acttttaagc tccctgtttc
     2281 agtccgtaat gatgcagaaa tggctgcttt tgctgaagca gcattaggag cgggtaaaga
     2341 attaaaaagc acttattttg tcactattag cactggcatt ggcggctgcc taattgttga
     2401 ccgcacaatt aaaaacccca gtccagaaat cgggcataca ctagtgcctt atcgcggtaa
     2461 tttttatgaa ttagaaaaga ttgcttcagg tgacggagta attaatcttg cagcgctaaa
     2521 tgagatatat tacgcaaatg cccaagcttt tttcaaagat gtcacttata aagtgcctta
     2581 tgccttaaaa atctttgatg aatggttaga tttgattact gacttcttca tttttgttaa
     2641 tgaagctttt acgcctgatg tgattgctgt tacgggcgga gtattaaaat cgaaaaaatt
     2701 attttgggac aaatttatcg aacgtgtccc taacgctaat attcaaatcg cgcattttag
     2761 tgaagatgcg ggattagtcg gtgcggcaag ttacggtttt aaagtatttg cctcacagta
     2821 actacttctt gcctatttag gcaaatatgt gtaaaattat ttaggcgaag acaaagacaa
     2881 gagcttaatg acttcactta tagagaccta gtgggtggtg caaactagga gggagcatta
     2941 atcttaccac tttcgagcct actaacgatg agttagtcgc tagtcacgtt acgactaaca
     3001 gagtgcatgg caacatgaat taaggtggta ccgcgcaaat atagcgtcct taaatgggcg
     3061 tttttttatt aaaaaggagg gtatatgaaa gaactcaaat ataacaatca actagtaaaa
     3121 gttaatgacg gcacaacagg ttttgactta cttaaaattt taaacatcga agggagaagt
     3181 aatgttttag tttataaact aaacggtgaa ctttttgacc ttgcaactcc agttgagcaa
     3241 gatggtgaat tttcctttgt gacaaaagag gacgaagaag cttttgcgat tttaaatcat
     3301 gacgctgccc acctcatggc acaagcaatt ttaagtattt atcctggcgc taaatttggg
     3361 attggtcccg atattagtga aggcttttat tatgatgttg attttggtga tgttgttata
     3421 aacgatggcg atttagcggt tattgaagcc aaaatgatgg aactctctag agcagaccac
     3481 cgtattgaac gccaagttgt tagtaaagaa aaagcattag aaatctttgc taaaaatgaa
     3541 tataaagttg aacttattaa tgaaattgag gagcgaatta ctatttattc gcaaggtgac
     3601 tttgttgatt tatgttacgg tccacatatg ccaagtacgc gttatgtcca acattttaaa
     3661 ctgcttagtt tagcaggagc ctattggcgt ggtgacgcaa gtcgtaaaca attaacgaga
     3721 atttatgggg tcgccttctt ctccaaagat gcacttgata agcatctcta taaccttgaa
     3781 gaagcaaaaa aacgtgacca ccgtaaatta ggtcgcgaac ttggactatt tatgcttagt
     3841 gaatatggtc caggcttccc cttctggctc caaaaaggaa tgactcttta tcacgaactt
     3901 gaacactggt ggattaaaaa acaacgcgaa gcaggttacg aatttgtcaa aacgccgcag
     3961 atcttaagtc gtgaactatg ggaaacaagc ggacactggc ttaattaccg tgaaaatatg
     4021 tacacaactc ttattgatga acgcgatttt gccattaaac cgatgaattg tcctggttca
     4081 atccttattt ataataatgg tttacattca tataaagatt tgccaatccg gatggctgaa
     4141 catggtcatg tccaccgtca tgaaccaagc ggttcactta acggattatt cagagtgcgg
     4201 agtttcactc aggatgatgc gcatatttat tgccgtaaag atcaattaca aagtgaaatt
     4261 aaacgtttgc ttaaacttta tgacgaagta tataaaacat ttaatcttag ttatcatatt
     4321 gaactttcaa caagacctga aaagaaatat attggtgact taaaaacatg gaatttaagc
     4381 gaaaaagcac tagcgaaagc gtgtattgct agcggtattc cttttaaaat taatgaagga
     4441 gatggtgctt tttacggccc aaaacttgac tttaaattgc gtgacagttt aggacgaatt
     4501 tggcaatgcg gaacgattca attagatatg aatttacccg aacgctttga tatttcttat
     4561 atcgacgcga ataaccaaaa agtacgccca attatgctcc accgcgctgt ctttggttcg
     4621 attgaacgct ttattggtat cttaattgaa cattacgctg gggccttccc gctctggcta
     4681 tcaccaacac aaattgctgt gttaccagtt aataacgatg cccatttaaa acacgctaaa
     4741 agtgttgtgc gcaaacttga aaaacaagga ctccgtgttg aacttgacga cagtaatgaa
     4801 aagttaggtt accgtttacg taatagtcaa attaataaaa ttccttatac gcttgtcatt
     4861 ggtgacaaag agcgaaatga taacttagtt acttatcgta aatttggtga taaagcacaa
     4921 attactgtac cttatcgtga atttatcacc cttatcaagc gtgaaattcg tgacaaagca
     4981 taacgcaaca caaactcctt cttctgtgag ataataatag agaaaaggag gaaaaattat
     5041 ggcatttaca ttaaaaccgc ttccatatgc ttatgatgca cttgaacctt atatcgatgc
     5101 aaaaactatg gaagttcacc attcaaaaca ccacgctaca tatgttgcta atttaaatgc
     5161 agcactagcg aaagatccat cgtttaacga ttggagttta gaaaaaatac ttaaaaactt
     5221 agataaatta ccagaaagca ttagaaccgc agttagaaat aacggaggcg gtgtctataa
     5281 ccatgacctc ttctggactt tactcaaagt taataacggc aaaggaccag aaggcaaact
     5341 taaagatgca ctagtggcaa cctttggcac ttttgaagct tttaaagatg cttttgctac
     5401 cgcagcaaaa tcacgctttg gctctggctg ggcgtggcta gtcgttacta gtgacaaaaa
     5461 actcaaggtc gttagcaccc caaaccaaga tattcccttt gatgaaggaa ccccaatctt
     5521 aacacttgat gtgtgggaac acgcttatta ccttaaatat caaaaccgtc gtccagaata
     5581 tgttgaagct ttctttaacg tcattaattg ggacgaagtc gcgcgtttat acgaaaatgc
     5641 attaaaataa caattaaatt tgacagatta tcatattaat gatatcttta tatcgtctaa
     5701 aagtggaaag tagaagcacc cgcttctcac cagatttact aggtaaattg gttgatgcta
     5761 cgaattaatc gttttatcgc gggtgtctac tgacgcccgc ttttattaag tacaaggagg
     5821 aaatgcttat ttacaaccaa aacagagaac ggcccgaacg caataccgat ttagttaatg
     5881 aaaacattcg gtttcctgaa gtcttagtca ttggtcctaa cggcgaacaa ctaggcgtca
     5941 tgaaaacacg cgctgctatt gacctagcgg catctgaata cgaactagat ctctactgtg
     6001 tgaacccgaa cggcaaacca ccagtttgca aaatgcttaa ttattctaaa caccgctttg
     6061 aacaacaaaa gcgcgctaaa gaacaaaaac ggaaacaaaa gactgtcgaa cttaaagaaa
     6121 ttcgtatgtc acccgtaatt gatgtacacg atcaagaaac aaaagggcgg atggctcgtc
     6181 gtttcttaga agatggtaat aaagtaaaag ttacaatccg ttatcgtgga cgccaaatgg
     6241 cccacattga tgttggtgaa aaaatattag aaagctttgt tgcatcacta gaggattatg
     6301 ctattgtcga acgaaaaccg ttaatggaag gtcgtaactt aagcgtcatc ttagcgagta
     6361 aaattaagaa ataggaggaa gtttcatgcc aaaaatgaaa agtaaaagtg ctttaaccaa
     6421 acggattaaa gtgactaaat caggcaaatt aatgcgtaaa gctgcatata catcgcattt
     6481 agccccccgc aaaacaacaa aacaaaaaag acaattacga aaagcgcgtt tagtgcataa
     6541 gagtgatgct aaacgtatta agtatttaat tcagaattag gaggaaggaa tcatggcaag
     6601 agtaaaaagt tcaccggtca cacgtgcccg tagaaaaaaa gttttaaaac aagctaaagg
     6661 ttactttggc gccaaaagct tactctttag aactgctaaa gagcaagtta tgcactcctt
     6721 acgttatgct tatatcgatc gtcgtcggat taaacgtgat tatcgtaaat tatggattaa
     6781 acgcattaac gccggctgtc ggatgtgtga aatcagttat agtcaattta tgcacggttt
     6841 aaaacttgca aatgttgata tcaatcgtaa aatgttaagt gaattagcaa ttaacgactt
     6901 tgaaacattc aaaggtttaa ctgaaacagc taaaaaagca ctcgcaaaat aatataaata
     6961 tttctttctt aataaaaatg ggagtagatt tatgtttact ccctttttta atgtttcact
     7021 tttcaataat tatagagtgt cacgaagagt aggtaaatct atactcataa agtcatataa
     7081 cttatatgta ttaagtgtgc caaatttact aactagattt ttagcatatt ctttaacagc
     7141 ggcaagagta tcgcctattc cccctttaaa gtataaaatt tcattaggta tgggttctaa
     7201 tatcttagca atcgctttaa ctgtatgcac actaaaggct attgcttctt tatgttcatg
     7261 aaaagcttgt tcatcatgga tacctagttt taaaaagcct tcataactac tttttatttc
     7321 ttgtttcaag tttttcatta tttcactttg attcatttaa atggccctct taattagtgt
     7381 tcctttttct ataaagtaag ctttaaacgg attatcttca atttcaagag cagaaattag
     7441 cgtcatattc tttatttcgc aaacacttct aatcacgagt tcgaaatatc gccaattata
     7501 aattgggagg tgagatctgc gtttaatgat gatgtaataa tgctcttctt ctttaaaggc
     7561 taaagcgtca cccatatcga acactcttaa gatatttggt aatttagtag ttaatcttcc
     7621 catattatct ttcttttaaa ataaatcttc ctttccatca tctaatctaa tgtcaacttt
     7681 tttcgtatca cgaattgctt cactgatgag agattcataa tcaaacttac tttcaaaaag
     7741 cactgcatct tctagtttag gactagttac tgagtttgta actgggaata ttgtacagca
     7801 atcttcataa ggtaaaattg aaattggata agtttcaatg tctttagcaa tttttataat
     7861 ttcattttta tcgagcgtcg ctaatgggcg aataattgga taattagtga cttcattaat
     7921 aacgacgagt gattcaagtg tttgacttga aacttgacca agtgaatcac cagtcactag
     7981 tactttataa ccgtgacgct tagcaatgac agtactaatg cgaagcatcg tgcgtcgcat
     8041 taaggtaata gcgtaaccac ttgtagcgtg ttcataaatc gctaattgga gtttagtaaa
     8101 tggtacgaca tataatctaa tatgcggttg atagttatta agttttgcgg ttagtgcttc
     8161 aattttcttt aatacttctt cacttgtata aggtggagcc gcaaagtgga ttgctgcaat
     8221 tttaattccg cgtctaatca ttaaataagc cgcaactggt gaatcaattc cgccactaag
     8281 gagcactaaa ccttttccag cagcaccaag cggatacccg cctaagccgt cataacgttt
     8341 accgacaaca agaatacgac ttttatcaat ttcaagacga agtggtaatt cgggattatg
     8401 gacatcaact ttgatttcag cattttttaa aatatgactc gcgacactgc gattaatttc
     8461 gtcgctcgtt aatggaaaaa gtttatcttt acgtttagta ataactttaa atgtttttgg
     8521 ctttagttca aggatgagtt caagcgcaaa ttgtttaact ttttctaggt ctaacgggaa
     8581 ttctattact tcactaattg catggattcc gctgacattt tgcaggcgtt ttgtaatttc
     8641 agcaacatct ttttctttga tgtttttaat ataaatatgg tcatgccttg tttcaatttg
     8701 tactttgaaa ttagcgacaa cttctttaat gttattagtc aattgacgaa taaaatcacg
     8761 acggtttttg cctttcgtat aaagttcacc aaaatgaatc attaacattt tactcacggc
     8821 catactcctt tataatttcg gttaatgtgt tacaaaatat ctcaacttca tcaagcgtat
     8881 tttccattcc aaaacttact cggattgagt tactagcgcg ttcttcatct tgcgaaatgg
     8941 catatactga cttactcatt gcatgtaatt tagcgctaca agagctagtc gtgccaacat
     9001 aaatatcgcg actttccaag gcgttgagca cgattgaagc tttaattgtc tttagtgaaa
     9061 agttaacaat atacggatta cttccgccga ccgaattaat aataaataaa tctttatgag
     9121 tagcaagaaa attatgtaat ttaagtgcta agcgataaga atgatcataa aatttatcca
     9181 tattaccttc agcgatacgc actgtttttg ctaatacaat attcgccgga gcattatgtg
     9241 ttcccggcct tatcccttct tcttgactgc cgccatacac tagtggcttt ggctttaagt
     9301 ttttccgtaa aagtagtgca ccacttcctt ttaatccgcc gattttatga gcggaaaatg
     9361 aataagcatc tgcagcgttg ggatgaaagt caagtttgcc taaggcttga gtgacatcag
     9421 tgtgaaatat aactttagga tattttttta gacatgctct aatctcccct aaatcaatga
     9481 catcaccaac ctcattatta acagccataa cggagacaag caccgtttta tcatttaata
     9541 gcgatttaaa aacgtcaaaa tcaaaaccac ctttaaagcg aatcggcact ttatgaatga
     9601 ttgccccacg ttctttctct aaatattcac atgcggcacg cacactaggg tgttcgtaat
     9661 cactaataat tatttcgtta ccacgatttt tataagcttc atataaacca ataagcgcaa
     9721 tattatttgc ttcagtagcg ccactagtga aaataacttt accatcacga aacttaaaca
     9781 ttcttaaaat ttcttcgcgt gatttatctt gtaatcttgc tatttcacgc gcaaaagtgt
     9841 gcatcccact tggattgccg taatattttt caagaatctc aatgtatgtt cttaaaactt
     9901 caggatgaat ttttgttgta ctagcgttat caaaataaat cattgtttac ggttttggta
     9961 tttctttaat tgttcgattg aattttcgta agtatgttta aaaagcccat taaagaagcg
    10021 ttgttcttca tcacggagtt ttgaatgtaa ttcactaaat tgtggacgga aagcgttaag
    10081 actaacgata acttcttccg ctgaatgagc gtactttaca tcagttgtaa tcacatcttc
    10141 taagcgtgta gcaagcgcac gtaattcatt tgttaaaact tcgacttggc tcacatcaat
    10201 tggtcgttca cgaataatcg tgctgatatc actaatcatt gcgtaaccac tatcaaaatc
    10261 ttgttcaaag ttttgaatat acgctgggac attaagctta cgcaaagtaa cttctaattc
    10321 tttaaagcga ataaataaaa gtttacaaat cgcaaagcca tcttcaacaa ttacacggag
    10381 tgattcaata tatgtttgaa attctctaac gagcacttca actttcatac tctcagcttg
    10441 cagttgttgg actttctcaa ctagcacact atagggttgt ttggttgcac tatggagaaa
    10501 agcatctaag ctacgcttaa ccatcatcat tgtattaaca ttagtgttag cttcttctaa
    10561 tttcttttca tattcaggat taattaaata atactctttc acttgcggca ttcgattaac
    10621 aagccgaata tagcgttgtt ctaattcatt aacatgtaaa tatgttttat cgtattctgc
    10681 cgtaaattcg cgttgttttt caatttcaac attgaaacta tttattaatt catcaattgt
    10741 atcggcaatt tgattaagtg tttgttcgac attgcgtgta tcaaaacgaa taagttgttc
    10801 tttaatcact tcgagctgtt cattaaagct ttcaatttca gcactgacac gtaaatggtg
    10861 aagtggaaca ttataagccg ttgcttcttc ataactcttc tttaatgtcg caattttttc
    10921 agggattaac actgtggcct tcacaactaa aagcggtaaa atttgtaaga tgcgttcaag
    10981 ttgatcatta aatgtccgaa tcttttctaa ataactacga gcttcgtcat aataagcacc
    11041 attaagagca ttatcatatt ctttaaaaag taattcaatc tgtgtaaata ttttttcaaa
    11101 gcttggtaca caaatttcca gttcatcttt atgatcacga taaatatttt taataatccg
    11161 taatttctct ttatcgtaaa cactctcttc cctaatttct gattcgggtt taatcatatt
    11221 cgttaaatct ttacttaact ctgtgacttt acgttcataa tcaagaatga tggggcgatg
    11281 ttcattaaaa gcttttttaa attctttatt acctgccgcg agtgcttcat ttaatatttg
    11341 aatactacga cttgctttac ggtcatcgac attgcgtaat tgttcaaagc gcgtaaagaa
    11401 ttcttcataa gctggacgca ttagtaaatt aatatcacta attgcttcga tacgtttaat
    11461 atattgaata ttttcaccaa ggagtaggct gcgcacatat gaatatttaa ttgataaaga
    11521 ttttaatgcc tttttatttt ttgtgccacg gaaggcaaat ttgcttaaaa taaatccgaa
    11581 aaccgctagg acaagaatga tacttgctac aatgaagaca atatcgagtg tgtttaataa
    11641 tgttactact ttcataagtc gcacctcact tattgtatat tatacaatgt ttgaaaatgt
    11701 tttcatacgt taacatatat ttggatagat gaataattaa aatt
//
LOCUS       DAOSLP010000033        11111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_5801_length_11111_cov_10.601031, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000033 DAOSLP010000000
VERSION     DAOSLP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11111
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5801_length_11111_cov_10.601031"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PK460_03820"
     CDS             complement(<1..972)
                     /locus_tag="PK460_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54238.1"
                     /translation="MTIYNYLKNYQPILFQTFGNALTTNNLSHAYLLDGEPGTPLKEV
                     AFYLAKSLVCDDPNPFACDKCLACHRFEEGNYTDFYFFDGSESSIKKDSVLELEKAFS
                     MTSVEHKGVLIYVIHNVENMTIEAINSLLKFLEEPAGKVYAFLTTNNLHNVIPTIKSR
                     SQILSLQLIKRDVLLKLCESIALPQDDIEMLVPFYNIPSLIEEEAKSERYPEYKRAVI
                     DTLETFSDNPKRGYLKAHELIVPLRERFVIKRVFTYFALFFKDVVNYNIGHERLFKSY
                     DPLIKKLAATIKNPLPLIVATYEISKKVDDYVNTGLLVDELLIKMVKE"
     gene            complement(969..1592)
                     /gene="tmk"
                     /locus_tag="PK460_03825"
     CDS             complement(969..1592)
                     /gene="tmk"
                     /locus_tag="PK460_03825"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOE54239.1"
                     /translation="MFITFEGPEGSGKTSIANRIIKTLIDKGYDIVHTREPGGTPIAE
                     QIRDVTLDKKNTALDARAEALLIAASRRQHVVEKIRPALAEGKIVICDRYLDSSLAYQ
                     GAARGLGIDTVLAINLFAVEDTLPNLTLLFDLEPAIGFERIKANKNREVNRLDLEERN
                     FYIKVRNAYLELSKKYKDRYRIIDASQSFEKVYEDTLKVILEYLPQK"
     gene            complement(1602..2201)
                     /gene="recR"
                     /locus_tag="PK460_03830"
     CDS             complement(1602..2201)
                     /gene="recR"
                     /locus_tag="PK460_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOE54240.1"
                     /translation="MDDLPTLNALIEHLKRLPSVGQKSAERMAQAFLTFDDETLKDFG
                     TVITKLKEKVHLCPNCGLYTEAELCSFCVDPNRNNKTLIVVSYPKDVLGFSKLEDYHG
                     RFHILGGVISPSSNKGVELLSIPELEKRIVRDNVEEIILATNPTVEGELTALFVSKKL
                     ADLPVKITRLAYGLPMGGQVDYADAMTLFKALEGRKDLK"
     gene            complement(2201..2506)
                     /locus_tag="PK460_03835"
     CDS             complement(2201..2506)
                     /locus_tag="PK460_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOE54241.1"
                     /translation="MSSSMQKLMQQAQKMKREMQKAQEALNAKEFSVTKGGGVTVAML
                     GSKEVVEVTIDEDLFDKDNKEMVETLIIMAFNECIEQINAEEAAINEAITGSATGLF"
     gene            complement(2499..4274)
                     /gene="dnaX"
                     /locus_tag="PK460_03840"
     CDS             complement(2499..4274)
                     /gene="dnaX"
                     /locus_tag="PK460_03840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOE54242.1"
                     /translation="MSYKALYRTYRPLTFGEIAGQRAIVRTLQNALNQNKIAHAYLFS
                     GPRGTGKTTMAKLFAKALNCEEGLGHQCNRCTNCNSVNDGSHPDVYEIDAASNNGVED
                     VRQLIENINYAPIRGRYKVYIIDEVHMMTQSAFNALLKTLEEPPQNVIFILATTEPHK
                     VIPTILSRCQRYNFSKVSDRDITKRLKTVLETEVIPYEEEALKLLVSLADGGVRDALS
                     MLDQVLAYSRQQLKLEDVLALFALTSKLEQLNLLRAVASSNVEEVFTILSGLIDKGVD
                     VRRLTTDLLNLLKDALIYYRTENTDLLNYLRKEEVQELNMMFEIENLNMMIENILKAQ
                     ADYRFASDVKNIFEITLLKIITSLNQDTMVEKELIIQKPKVKTAPSVPKKDEKHAIVT
                     ETEKETIVEEQELPPFMTQSREKLEKRVTETKSEIVKDEVKTAQPVPEKVQYANPLHN
                     EGQVIELDDDTIIKILTLADRDEKLSLRNNRWDDLALMAMDSVVGEHANVLLNGAPYA
                     LAPEIILVEFNFDQYARYINILENQKIMQDIIERLVGRRIPVYGLTHGQAVSFARKYR
                     SMSQIQKLPRVKSVKEELEGKFDYV"
     gene            4554..5513
                     /locus_tag="PK460_03845"
     CDS             4554..5513
                     /locus_tag="PK460_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE54243.1"
                     /translation="MFNFQLSSNKPQINYDELYDFIALGLGPAGLNAGLYAHRKGLKT
                     LIVGHRVGGQLLNTADIDNYLGFSNIPAQKLIDEFRNHIENSKVPMLTDVLVTNIEKS
                     ADIFIITLNNGKTLQSKTVLYALGGSPRKLKVPGEDRLAGKGVAYCATCDGPFFQDKD
                     VIIAGGGNSAIDATKDLARIAKSVTIIEKFTISADKTSVEQVAKLPNVTMFEETDVTE
                     IIGDKYIEGIRVFDRKANKERIINTEGLFVEIGSIPNTALLKDLVELNKWGEVIVNNK
                     QETSLKGLYAAGDVTNNWIRQVIVAAADGAKAALEANNYITKK"
     gene            5524..6168
                     /locus_tag="PK460_03850"
     CDS             5524..6168
                     /locus_tag="PK460_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOE54244.1"
                     /translation="MGKLFNEEVASQIKGVLAQMKNEITIKLFIDGKCDTCLETKQLL
                     EETKTLSDKINLVVHEINESKEEAEKYDITLTPTFIILDDKGTYSGVRFSGFPGGYEI
                     NSFVSSLLEMSGLRHEFPQEVVDRVAKIKEPIDIKVFVTLGCPHCPGAVETAHRLAML
                     NSNIKATMIESQTFMELARTHKVSSVPKIVFNDALDLLGNQPVEAFLDEIETLL"
     gene            6283..7356
                     /gene="prfA"
                     /locus_tag="PK460_03855"
     CDS             6283..7356
                     /gene="prfA"
                     /locus_tag="PK460_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOE54245.1"
                     /translation="MEDVLRNRLETTKKRLSEIDEELLKPDITSDLDYFRDISVERSQ
                     IEEVVEKYNEYLKLEEDLESSLILMDDKDEEIAEFAKEQHKEIANRLPELINEIRVLL
                     IPKDPYDDKNIVVEIRGAVGGDEANIFAGDLFRMYVKYAERQGWQMQILDENSSAVGG
                     FSQISFMIKGKGVYSKLKYESGGHRVQRVPKTEASGRIHTSIATVLVMPEAEEVDVEI
                     RNEDLEIDTFRSQGAGGQNVNKVESAVRITHKPTGISVACQVEKSQIQNREIAMRLLR
                     ARVLAMKVAEQEEKIGAERKLKVGTGERSEKIRTYNYPQNRVTDHRIGFSTLKLDLVL
                     EGNLEEIIEALVHYDEQQKMVNS"
     gene            7356..8186
                     /gene="prmC"
                     /locus_tag="PK460_03860"
     CDS             7356..8186
                     /gene="prmC"
                     /locus_tag="PK460_03860"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOE54246.1"
                     /translation="MLTLYSLKQNTLKLHNFLDKNDVKLALMHVYNIDSYTDYVLKLH
                     TPIEEKQEFYAIINRLVKGEPIQYILGKAYFYDRYFFVNKNVLIPRVETEELVDIVLK
                     DVKENTGNLLDLGTGSGAIALTLKAHSKLSVFASDISKKALEVAQKNDCENSVTFVHG
                     DLLAPFINKEIAFDYIVANLPYIKREEEVEQKVKDYEPESALYNSDPNIFERLFKEVK
                     MLNFPKTGLSIYLEIGTNQDDEVSNLARRYLGENVKINVVNDMHGKKRFVKIKDIYGN
                     "
     gene            8176..8790
                     /locus_tag="PK460_03865"
     CDS             8176..8790
                     /locus_tag="PK460_03865"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOE54247.1"
                     /translation="METKIFKANEIHLLANIAKEGSIIAFPTETVFGLGVVYDNEEAF
                     NNLVKVKERKTDKPFTLMVGYTRDIENYAVLNTKTKNLIDVFMPGEITLIIEAKPNLP
                     KHVTLGSKYIGIRVSANYEVANLLNLIGKPLLVPSANKRDEKPALTVEEVYKVFNGDI
                     AGILEGETTSHIPSTIVKVDDKIELIREGSIPFNLIKEVWEEEL"
     gene            8787..9230
                     /gene="rpiB"
                     /locus_tag="PK460_03870"
     CDS             8787..9230
                     /gene="rpiB"
                     /locus_tag="PK460_03870"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOE54248.1"
                     /translation="MKVAIGSDHGGFIYKEAVKKHLEDKGITVIDVGSYHEDSVDYPL
                     YGIAVGEKVRDKAADLGIVICTSGEGITIAANKVKGVRAGIGYNDDVSRLIREHNNAN
                     VIGFGGSQMELKDVLRRVDIFLNARFESGGRHSRRVGIITEYENK"
     gene            9271..10440
                     /locus_tag="PK460_03875"
     CDS             9271..10440
                     /locus_tag="PK460_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOE54249.1"
                     /translation="MQDFFICPTCGNNDLNKIGVRNGQKYCRACLPFQGQNAPKITNI
                     PLHIPITLSYELTTEQRRIAEEINANYINKQNSLVYAVCGAGKTELVYLAMQTALARG
                     HQIGFTVPRREVVIELAMRIKKTFPQARVISVFGDHHDALTGNIIVLTTHQLFRYEDY
                     FDLLIFDEIDAFPYVGDLVLESFFKRSVKGVYVLLSATPSTKLLKEFPKVGKVLTLFT
                     RFHNHKIPVPIVKRGFLLFNFMLLLKHLESFIKRKKPVLIFVPTIRLANLIYKLLRID
                     YKNGNVVHSKVKDNSAIIDDFKKGHYAYLVTTTVLERGVTIKGLQVIIYGADHAVYDT
                     ANLVQISGRVGRKKEESDGEVIFIVTKKNAAIKAAISDIEEKNAYLQSVLQRKAT"
     gene            10415..11005
                     /locus_tag="PK460_03880"
     CDS             10415..11005
                     /locus_tag="PK460_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOE54250.1"
                     /translation="MCFKEKQPNFNDLIRDTNVCNKCFRDLDVKFNVSKIAGVTYISV
                     FNYDEHFKTLLFHLKGLKDIVLAPLFLERFIPYFKVRFINYYLVPAPSTIESDKERGF
                     NHVIEIFKPLQRPFLRLITKKVAFKQSDLNYALRQKVKDKLVINNGNKVKGKNILIVD
                     DIKTTGATIMAMINLIKPYNPRKIAILTLAETSLSE"
BASE COUNT         3766 a         1786 c         1976 g         3583 t
ORIGIN      
        1 ttcctttacc attttgatta ataattcatc cacgagtaag ccagtattaa cataatcatc
       61 aaccttttta cttatttcat aagtagcaac aattaatggc agtggattct ttatcgtcgc
      121 tgctaacttt ttaataagcg gatcataact cttaaaaagt cgctcgtgac ctatattata
      181 gtttacaaca tctttgaaga aaagggcaaa atacgtaaat acacgcttaa ttacaaagcg
      241 ttcacgcagc ggaacgatta attcgtgggc ctttaagtag ccgcgcttag gattgtcgct
      301 aaaagtctct aaagtatcaa ttacagctct tttatactct ggatatcgtt ctgattttgc
      361 ttcttcttca attaaactag gaatattata aaaaggcaca agcatttcta tatcatcttg
      421 cggtaacgct attgattcgc aaagttttaa gagtacatcg cgtttaatta gttgtaatga
      481 aagtatttga cttcgactct taatagtcgg gataacatta tgtaagttat ttgtagttaa
      541 aaaagcatat acttttcccg ctggctcttc gagaaacttt aaaagtgaat taattgcttc
      601 aatagtcata ttttcaacat tatgaatcac ataaattaaa acacctttat gttcaacact
      661 agtcatgctg aaggcttttt caagttctaa tacactatct tttttaatac ttgattctga
      721 gccgtcaaaa aaatagaaat cagtgtaatt accttcttca aaacgatgac acgctaagca
      781 tttatcacac gcgaaaggat taggatcatc acacactaaa ctttttgcta aataaaaagc
      841 aacttcttta agtggtgttc caggttcacc atcaagcaaa taagcgtgac ttaaattatt
      901 tgtagttaaa gcatttccaa atgtttggaa taaaataggc tgataatttt ttaaataatt
      961 ataaattgtc atttttgtgg gagatattct aaaataactt ttagtgtatc ctcataaact
     1021 ttctcaaaac tttggcttgc gtcaattatt cgataacggt ctttatattt tttacttagt
     1081 tcaaggtagg cattgcgcac cttaatgtaa aaattgcgtt cttctaagtc taggcgatta
     1141 acttcacgat ttttattagc tttaattcgt tcgaaaccaa ttgctggctc taaatcaaat
     1201 aaaagtgtca aatttggcaa ggtgtcttca actgcaaaaa ggttaattgc caggactgtg
     1261 tcgattccta aacccctagc cgctccttga taggcgagcg aagaatctaa ataacggtca
     1321 caaataacaa tttttccctc ggctagtgct ggacgaattt tctcaacgac atgttgcctt
     1381 ctacttgctg caattagaag tgcttctgcc cgagcatcaa gcgccgtgtt tttcttgtct
     1441 aaagttacat cacgaatttg ttcggcaata ggagtgccac caggttcacg tgtatgaaca
     1501 atatcataac ctttatcaat aagtgttttt ataattctat tagcaatact agtttttccg
     1561 cttccttctg gtccttcaaa tgtaataaac ataacaccct cctatttaag atcttttcgt
     1621 ccttctaatg ctttaaataa agtcatagca tcagcgtaat ctacttgccc gcccattggc
     1681 agtccataag ccaatcgtgt aatttttaca ggtaaatccg caagtttttt actaacaaag
     1741 agtgctgtta gttcaccttc tactgtcgga tttgtggcaa gaataatttc ttcaacattg
     1801 tcacgtacaa ttcttttttc taactctggg attgaaagaa gttcaacgcc tttatttgaa
     1861 gaaggactaa taacgcctcc taatatatgg aagcgcccat ggtagtcttc taatttacta
     1921 aaacccaaaa catccttagg ataactaacg acgattaaag ttttattgtt acgatttggg
     1981 tcaacgcaaa aagaacataa ttccgcttct gtgtaaagac cacaattagg acacaggtgc
     2041 accttttctt ttaatttagt gataactgtg ccgaaatctt ttaatgtctc atcatcaaat
     2101 gttaaaaaag cttgtgccat acgctcagca cttttttgac caacacttgg taaacgtttc
     2161 aagtgttcaa ttaatgcgtt tagtgttggt aaatcatcca ttaaaatagt cctgttgcac
     2221 ttcctgtgat tgcttcatta atagcggctt cttctgcatt tatttgttca atacattcgt
     2281 taaaagccat aatgataagc gtttcaacca tttctttatt atctttatca aaaagatctt
     2341 cgtcaattgt cacttcgaca acttctttac ttccaagcat agcaacagtt acacccccac
     2401 ctttagtaac ggagaactcc tttgcgttta atgcttcttg tgctttttgc atttcgcgct
     2461 tcattttttg cgcttgttgc attaattttt gcatactact agacataatc aaatttccct
     2521 tctaattctt cttttacgct cttaacacgc ggtaattttt gaatttggct catcgagcga
     2581 tattttcttg caaaactcac agcttgacca tgggtaagcc catagactgg tatgcgacgc
     2641 cccactaacc tttctataat atcttgcata attttttgat tttctaaaat attaatataa
     2701 cgagcatact gatcaaaatt aaattcgacg agaatgattt ctggggctag ggcataagga
     2761 gcaccattaa gcaatacatt agcgtgttca ccaactacac tatccatagc catcagcgct
     2821 aaatcatccc aacgattatt tctaagtgac aacttttcat cgcggtcagc tagtgtaagt
     2881 attttgataa tagtgtcatc gtctaattca ataacctgac cctcattatg taaaggattt
     2941 gcatattgga ccttttcagg cacaggctgg gcagttttca cttcgtcctt aactatttct
     3001 gatttagttt cggtgacgcg cttttctagt ttttcccttg actgtgtcat aaaaggtggt
     3061 aattcttgct cttcaactat agtttctttc tcagtttcag taactatagc gtgtttttca
     3121 tcctttttcg gcacagatgg ggcagttttt acttttggtt tctgaataat taactctttt
     3181 tcgaccattg tgtcttgatt taatgatgtg ataattttta aaagtgtaat ttcaaagata
     3241 tttttgacat cacttgcaaa ccgataatct gcttgagcct tcaatatgtt ttcaatcatc
     3301 atgtttaagt tttcaatctc aaacatcata tttaattctt gcacttcttc tttgcgcaag
     3361 taatttaata aatcagtatt ttctgtccga taataaataa gtgcatcttt taataaatta
     3421 agtaaatctg ttgttaaacg tcttacatca acacctttat caattaaacc tgatagtatt
     3481 gtaaaaactt cttcgacatt actactagca actgctctta ataagttaag ttgttctagt
     3541 ttactagtta gggcgaaaag cgctaataca tcttcaagtt ttaattgttg acgagagtaa
     3601 gctaaaactt ggtctaacat gcttaaagca tcacggacgc ctccgtctgc caacgacact
     3661 aataatttta gtgcttcttc ttcataagga ataacttcag tttctaaaac tgtttttaaa
     3721 cgtttagtaa tgtcgcgatc acttactttt gagaagttat agcgttgaca gcgacttaaa
     3781 atcgttggaa ttaccttgtg tggctcagtt gttgccagaa taaaaataac attttgtggt
     3841 ggctcttcaa gtgttttaag caaagcattg aatgcacttt gtgtcatcat atgaacttcg
     3901 tcaatgatat atactttata acgacctcta atcggcgcat aatttatatt ttcaataagt
     3961 tgacgaacat cctcaacgcc attattactg gcggcgtcaa tttcatatac atcaggatgg
     4021 ctgccatcgt taacactatt acaattagtg caacgattac attgatgacc aagaccctct
     4081 tcacaattaa gggctttagc aaagagttta gccatcgttg ttttaccagt accgcgcggt
     4141 ccactaaaaa gataagcatg tgcaatctta ttttgattta gggcattttg taaagtgcgg
     4201 acaattgctc tttgtccagc tatttcgcca aaagtaagtg gccgatatgt tcgatatagt
     4261 gctttataac tcatgtagta attatacaat aattaagact acattgtagg aataagaaca
     4321 caaaacttaa attgttttga taatatgcag gatgatgact ttttattgct tcataatgtg
     4381 tttcattctg caattagttt aatgtttctt tatattaata attgttataa attttattgt
     4441 ctaaaaacat aaaaacacgc attttatgta gcgacacttt ttgcattaag caaattgttt
     4501 ttatttaagt taatcgacct atataatatc ggcagaaagg agaaaactat attatgttca
     4561 atttccaact aagctcaaat aaacctcaaa ttaattatga tgaactctat gattttattg
     4621 ctcttggatt aggtccagct ggtctaaatg ctggattata cgctcatcgc aagggcttaa
     4681 aaactctaat agttggtcat cgtgttggcg gtcaattatt aaatactgct gatattgata
     4741 attatctagg tttctcaaat attcccgcac aaaaattaat tgatgaattt agaaatcata
     4801 ttgaaaattc aaaagtacca atgctcactg atgtgttagt tactaacatt gaaaaaagtg
     4861 ctgatatatt cattattaca ctcaataacg gcaagacttt acaaagtaaa accgtgttat
     4921 atgctcttgg gggtagtccg cgcaagctaa aagtgcctgg tgaagataga cttgctggta
     4981 aaggagtcgc ttactgcgct acgtgtgacg gaccattctt tcaagataag gatgtaataa
     5041 tcgcaggtgg gggtaatagt gcgatagacg ccacgaaaga cttagcgcgt attgcaaaaa
     5101 gcgtgacaat tattgaaaaa ttcacgataa gcgctgataa aactagtgta gaacaagtgg
     5161 caaaattacc aaatgtaacg atgtttgaag aaactgatgt tacagaaatc attggtgata
     5221 aatatattga aggaatcaga gtgttcgaca gaaaggcaaa taaagaaaga attattaaca
     5281 ctgaaggctt atttgtcgaa attgggagta ttccaaatac cgctctatta aaagaccttg
     5341 ttgaattaaa caagtggggc gaagtaattg ttaataacaa acaagaaaca agtcttaaag
     5401 gtctatatgc agcaggagat gtaactaata actggattcg tcaagtcatc gttgctgcag
     5461 ccgatggagc caaagctgct ttagaagcaa acaactatat tacaaaaaag taaaggagaa
     5521 attatgggga aattatttaa cgaagaagtt gcaagccaga ttaaaggcgt gcttgcacaa
     5581 atgaaaaatg aaattacaat caaattattt attgatggca aatgtgacac ttgtttagaa
     5641 acaaaacaac tattagaaga aactaaaaca ttaagcgaca aaatcaatct tgttgttcac
     5701 gaaattaacg aatcaaaaga agaagcggaa aaatatgata ttacgcttac tccaacattt
     5761 ataattctcg atgataaagg aacatatagc ggagttagat tcagtggttt tccaggaggt
     5821 tatgaaatta attcattcgt tagttcactt ttagaaatgt ctggattacg ccacgaattc
     5881 ccacaagaag tagtggaccg tgttgctaaa attaaagagc caattgacat taaagtattc
     5941 gtgactctag gttgtccgca ctgtccaggt gctgttgaaa ccgctcatcg tttagcaatg
     6001 ttaaatagta acattaaagc aacaatgatt gaatcacaaa catttatgga gttagcgcgt
     6061 acacataaag tttctagcgt tccaaaaatt gtgttcaatg atgcacttga cttacttggt
     6121 aatcaaccag tggaagcttt cttagacgaa atcgaaactt tattatagaa acacttacat
     6181 taaggctcct actactatta ggggcctttt tcttatataa ataagaagat ataatgaaaa
     6241 agcattaaaa atataatata atacgaccgc aaggataaat atatggaaga tgtattaaga
     6301 aatagattag aaacaacaaa aaagcgttta tcagagattg atgaagagtt acttaaaccg
     6361 gatattacaa gtgaccttga ttattttcgt gatatttccg ttgaacgctc acaaatagaa
     6421 gaagttgtgg aaaagtacaa cgaatattta aaattagaag aagatttaga atcttcctta
     6481 atcttaatgg atgataaaga cgaagaaata gcagaatttg ctaaagaaca acataaggaa
     6541 atcgctaaca gattaccaga attaatcaat gagattaggg tgcttttaat tccaaaagat
     6601 ccttatgatg acaaaaacat tgttgttgaa attagaggcg ctgttggggg agacgaggca
     6661 aatatttttg cgggtgatct atttagaatg tatgttaaat atgctgaaag acaaggttgg
     6721 caaatgcaaa tccttgatga aaatagtagc gccgttggtg gcttttcgca aatctccttt
     6781 atgattaaag gtaaaggtgt ttattcaaaa cttaaatatg aaagtggtgg gcatcgtgtg
     6841 cagcgtgtac caaaaactga agcaagcgga agaatccata cctcaatcgc tactgtgtta
     6901 gtgatgcctg aagcagaaga agttgatgtt gaaattcgta atgaagattt agaaattgat
     6961 acttttagaa gtcaaggcgc tggtggacaa aatgttaaca aagttgaatc cgctgtacgt
     7021 attacgcata aaccaactgg tatcagtgtt gcttgtcaag tggagaaaag ccaaattcaa
     7081 aatcgtgaaa tagcaatgcg tttattacgc gcccgtgtct tagcgatgaa agttgctgaa
     7141 caagaggaaa aaatcggtgc tgaacgtaaa cttaaagtcg gcactggcga acgaagtgaa
     7201 aagattagaa cttataacta tccgcaaaat agagttactg atcaccgtat tggctttagc
     7261 acattaaaat tagatttagt gcttgaagga aatttagaag aaattattga agcgctcgtg
     7321 cattatgatg aacaacaaaa aatggtaaat agttaatgtt aactttatac tctttaaaac
     7381 aaaatacatt aaaactacat aattttctcg acaaaaatga tgtaaaatta gcattaatgc
     7441 atgtttataa tattgattcg tatacagatt atgttttaaa attgcatact cccattgaag
     7501 aaaagcaaga gttttatgca attattaatc gtcttgttaa aggcgaacct attcaatata
     7561 ttttaggtaa agcttatttt tatgatcggt attttttcgt aaataaaaat gtgttaatac
     7621 cgcgtgttga aactgaagaa ttagtggata ttgtgcttaa agatgtaaaa gaaaatacag
     7681 gtaatttact tgatttaggt acaggaagcg gtgcgattgc attaacttta aaagctcatt
     7741 ccaaattaag tgttttcgct agtgatataa gtaaaaaagc attagaagtc gcccaaaaaa
     7801 acgattgtga aaatagtgta acttttgttc atggcgactt attagcgcca tttattaata
     7861 aagaaatcgc ttttgattat attgttgcga atttacctta tataaagcgc gaagaggaag
     7921 ttgaacaaaa agtaaaagat tatgaacccg aaagcgcact ttataatagt gatccaaata
     7981 tttttgaaag actatttaaa gaagttaaaa tgttaaactt tcccaaaaca ggattatcaa
     8041 tttatttaga gattggcact aatcaagacg acgaagtaag taatttagcg cgccgctact
     8101 taggagaaaa tgttaaaatt aatgttgtga atgatatgca cggtaaaaag cgctttgtca
     8161 aaattaagga tatttatgga aactaaaata tttaaagcaa atgaaattca tttattagca
     8221 aacattgcga aagaagggag tattattgct tttccgactg agacagtatt tggtcttggt
     8281 gttgtttatg ataatgagga agcctttaac aatttagtga aagttaaaga acgtaaaact
     8341 gataaaccgt ttaccttaat ggttggttat acacgagata ttgaaaatta tgcggtttta
     8401 aacactaaaa ctaaaaattt aattgatgta tttatgcctg gagaaataac tttaattatt
     8461 gaagcaaaac ctaatttacc gaaacacgtc accctaggtt ctaaatatat tggcataaga
     8521 gtaagcgcta attatgaagt tgctaatctt ttaaatttaa ttgggaaacc actacttgta
     8581 cctagcgcta ataagcggga tgaaaaacct gctttaacag ttgaagaagt atataaagtc
     8641 tttaatggtg atattgccgg cattcttgaa ggcgagacga catcgcatat tccgtccaca
     8701 atcgttaagg tggatgataa aatagaatta attagagaag gaagcattcc ctttaattta
     8761 attaaagaag tttgggagga agaactatga aagtagcaat tggcagtgat catggtggct
     8821 ttatttataa agaagcagta aaaaaacatt tagaagataa agggattacc gtaattgatg
     8881 tcggttcata tcatgaagat agtgtagatt atccgcttta tgggatagct gttggtgaaa
     8941 aagttcgtga taaagctgct gatttaggaa tagttatttg tacatcaggc gaaggcatta
     9001 ctattgccgc aaataaggtt aaaggggtac gcgctggtat tggttataat gatgatgttt
     9061 cgcgtctcat tcgtgaacat aataatgcca atgtaattgg ttttggtggt tcacaaatgg
     9121 aattaaagga tgttttacgc cgtgtcgata tttttcttaa tgctagattt gaaagtggtg
     9181 gtcggcattc gcggcgtgtc ggtatcatca ctgagtacga aaataaataa ttactgataa
     9241 ataaagggtc ttgcttacat acttataaat atgcaagact tttttatttg cccgacttgc
     9301 gggaataacg atcttaataa aataggagta agaaacggcc aaaaatattg ccgagcatgt
     9361 ttacccttcc aagggcaaaa cgcgcccaaa ataactaata ttccactgca tataccaata
     9421 acactaagtt atgaattaac aacagaacaa aggcgaattg ccgaagaaat caatgcaaat
     9481 tacataaata aacaaaattc attagtgtac gctgtatgtg gcgctggtaa aactgaatta
     9541 gtgtatttag caatgcaaac tgcactagcg cgtggccatc aaataggttt tacagttccg
     9601 cggcgtgaag tcgtaattga gttagcgatg cgcattaaaa aaacttttcc gcaagcgaga
     9661 gtaatcagtg tttttggtga ccatcacgat gctttaactg gcaatattat tgtacttact
     9721 actcatcaac tttttagata tgaagattat tttgatttat taatttttga tgaaattgat
     9781 gcttttcctt atgttggcga cttagtgctt gaatcatttt ttaaacgcag tgtcaagggt
     9841 gtatatgttt tactaagtgc tactccgagt acaaagctat taaaagagtt tccaaaagtt
     9901 ggtaaagtgt taactctttt tacaagattt cataatcata aaatcccagt gccaatagtg
     9961 aaacgcgggt ttttactatt taattttatg ttgttattaa aacatttaga atctttcatt
    10021 aaaagaaaga aaccagtttt aatttttgta ccaacgataa gactcgctaa tttaatatac
    10081 aaactattac gcatcgatta taaaaatggt aatgtagtgc attcaaaagt aaaagataat
    10141 tcagcgataa ttgatgattt caaaaaagga cattacgcct acttagtaac gacaacagtc
    10201 ttagaaagag gggtaacgat taaagggtta caagtaataa tttatggcgc agatcacgca
    10261 gtgtacgaca ctgctaattt agtgcaaatt tctggaagag tcggacgaaa gaaggaggag
    10321 agtgatggtg aagttatttt catcgttacg aaaaagaatg cggcaatcaa agcagcaatt
    10381 agtgatattg aagaaaaaaa cgcctatttg caaagtgtgc ttcaaagaaa agcaacctaa
    10441 ctttaatgat ttgattcgtg acactaatgt ttgtaataaa tgttttcgtg atttagatgt
    10501 taagtttaat gtttcaaaga ttgcaggagt tacttatatt agtgttttta attatgacga
    10561 acattttaaa acactacttt tccatctaaa aggtttaaaa gacattgtgc tagcgccact
    10621 ttttcttgaa aggtttattc cttactttaa agtacggttt attaattact atttagtgcc
    10681 agcgccttca acaatagaga gtgataaaga acgcggattt aatcatgtca tcgaaatatt
    10741 taaaccgcta caaagacctt ttttaaggct tattacaaag aaagttgcct tcaaacaaag
    10801 tgacttaaac tatgcactcc ggcaaaaagt aaaagataaa ttagtaatta ataacggaaa
    10861 taaagtaaaa ggtaaaaata ttttaatcgt tgatgatatt aaaacaacag gtgccacaat
    10921 tatggcaatg attaacttaa taaaacccta taatccgcgg aaaattgcaa tattaacact
    10981 agcggaaaca tcgctaagcg agtaactatc atatttttat agaaaatatg gtaaactttt
    11041 atagatacat gatacgaagg agattaacta ttcttaatca agttaaatag taaatatata
    11101 tcaaaaagat t
//
LOCUS       DAOSLP010000034        11099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_5812_length_11099_cov_7.603948, whole genome shotgun sequence.
ACCESSION   DAOSLP010000034 DAOSLP010000000
VERSION     DAOSLP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5812_length_11099_cov_7.603948"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /gene="alaS"
                     /locus_tag="PK460_03885"
     CDS             <1..2079
                     /gene="alaS"
                     /locus_tag="PK460_03885"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOE54251.1"
                     /translation="FDLGGKIDARGPELLASDIDNDRFLEIWNIVFSQYNAKAGVKRE
                     NYEELPSKNIDTGAGLERIASVLQEVPSNFETDLFMPIIKELEKLSGVKYSDDIVSFR
                     VIADHIRTSTFAIADGASFSNEGRGYVLRRVLRRAVRYGQKLAMKEPFLYKLVQSVVD
                     VMKDFYPYLESKIDHVEKLIKAEEVRFLNTLNHGEELLRGALSNSKNISGEVAFKLYD
                     TFGFPIDLTLEIASENGASVDVKGFEVLLEEQRERARAARKNLNSMASQSEDLLKFDQ
                     KSKFEEQKFSVTATVIGLFKDGVKVNESAEDVSAIFDVTPFYSESGGQVSDSGFVSNK
                     ELRAVVSAMSKAPHGQHLHHLTLDYGVIKVGDKVQLEIDGVARRSIMLNHSATHLLHR
                     ALNDVLGSEVSQAGSYVGPDYLRFDFNYNTRVTDEELEKIELIVNKYISDSVPLTVKY
                     MTLAEAQKLGAKALFMEKYGDLVRVVTFAGISVELCGGTHIDNTSKIGTFVIVSESSI
                     ASGVRRIVALTGLAAYEHLKTYSDTVKRISKELGLNDKKDVVPHVRTLVEKNALLNSE
                     VTMLKTAELHRTASALTPKKHGDTNIYLEYFTTSNREDLGVIADKVRSTDECALLVLV
                     GNKTNDFPLLISVSKELSKSLNANKVMRALTSAFGGRGGGRPDFANGVLLNLPEKTKA
                     LATILKVLDE"
     gene            2072..2506
                     /gene="ruvX"
                     /locus_tag="PK460_03890"
     CDS             2072..2506
                     /gene="ruvX"
                     /locus_tag="PK460_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOE54252.1"
                     /translation="MSKILGLDLGTKTLGIAISNVDATFAVAYENYVFPPHYYKKARA
                     HVLAICAKENISEIALGYPLNTDGTVGERAKSAERFRDDLLREGSPPIVLVNETYTTL
                     AATEELERLGYKKEQIKAQIDAVAAKYILETYLERKRSNERK"
     gene            2493..2741
                     /locus_tag="PK460_03895"
     CDS             2493..2741
                     /locus_tag="PK460_03895"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOE54253.1"
                     /translation="MKENDVIVITTEEGGEEEFIILFTYENEERGTKYVFFYDENNDE
                     EVMCAKYFDDGTLEYVEDEEELAEMEEVFRAYESELEE"
     gene            2821..3300
                     /gene="greA"
                     /locus_tag="PK460_03900"
     CDS             2821..3300
                     /gene="greA"
                     /locus_tag="PK460_03900"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOE54254.1"
                     /translation="MTNKYTLTQEGVDALEEELSVLVNVERPLVLKEIEEARSFGDLS
                     ENADYDAARNKQANIESRIMEIEHILAHKEIVEYSKTSKTVAVGNYVEVHDRTNDDTF
                     SLRILGQFEGKPLNGTVSVDAPIAQAILGKKVGDRVNVKAQPPYEVEILNISTEPIE"
     gene            3363..3899
                     /locus_tag="PK460_03905"
     CDS             3363..3899
                     /locus_tag="PK460_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOE54255.1"
                     /translation="MLKLMIGVVIATVIVIFAFSVINSNMSANPQGQSSMHVIDDNFL
                     SITVTGQVVKPGTYVMKKDETIGDLIMAAGGPTSNADARSYIEDTVLIAGVSYYIAPI
                     NDLDDYCGEMPLHKVNINADPKDKLMEVNGIGETLAGAIIQYRGEHGEFTYLEELMKV
                     KGIAKATFEKIKNHVTLR"
     gene            4055..5893
                     /locus_tag="PK460_03910"
     CDS             4055..5893
                     /locus_tag="PK460_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOE54256.1"
                     /translation="MIISNFISKLPFESSFGLITKAGTNHIIVLTVRGKFYVRLENNK
                     LVEGDIILLNGYKVAYFFKRLEGEFNYAKYLENKGIKSALNLSFYELKVPSLLRLFNK
                     FTVKKNTYPPHLEHIKNLLLIKTINYDDPFIHELIRLDLLFLISLTGAHLSFLRRVVK
                     KISSVFVNEFLQEVISYLCLIPLFLLNITKFAFYRIFLTGILRLINKKHLNAYFNSVE
                     LTCIAAFFFLVINPYFVFDPAFYLAYLLTFLFQMVRPSRSVKGRLQSSLIVMCVIVPY
                     NIFTSGTFNLVYIFISLLLVPLIAVWTLVYFVTLFIAPLQNVGINTLNLIYHLVVLMK
                     KINITLYFGDLNVLSKLLLVTFISVLLYAFNAKIKPLQKDITLLITTLLLLNLLPLNH
                     YFEYSISFINVGQGDSTLLRIKNKYVLIDTGGLTYKDVGNDILIPYLKRNKIYRLDYL
                     IITHDDFDHCGAKEALMHNFTVKNVITERQYFPLNVNGITLENLNNKYYDDVNLDSLI
                     IYVPFPKLKILLMADAGIPNEVDLIKTYPTLEVDVLKVGHHGSNTSTSDDFIRHYKPQ
                     VAIISCGYQNKYGHPHHTVLSTLQKYDVKIKRTDTLGTITLKTCII"
     gene            5896..6846
                     /gene="holA"
                     /locus_tag="PK460_03915"
     CDS             5896..6846
                     /gene="holA"
                     /locus_tag="PK460_03915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOE54257.1"
                     /translation="MLYLFAANEYALVEKVIRKTVDALLPERNAFNYVRYDMRETPFS
                     EIIEDALSYAFDSSVRVIVIDHASVLTTSNEKSINKDIPDEQIDLLSRLSEDTHLIFI
                     ARSSQINPKHKIIKFIENNGKITVEKDIDKNDWQRFVRAYFAKENLPIDDEAASELLS
                     RVTNLGSFLQEAEKLAIYGKRVTLPLVEELVTPLLEENSFALVNAFISGDKAKTIKIY
                     QDFKVQNQEPVAFIAQVGNQFRTYAQIYICHEEGMTNEEIASFLKIHPYHVKLAMDQR
                     RRTSLEEVFNILLALADLDYKIKSGQIDRFYGFEMFLLNY"
     gene            complement(6843..7109)
                     /gene="rpsT"
                     /locus_tag="PK460_03920"
     CDS             complement(6843..7109)
                     /gene="rpsT"
                     /locus_tag="PK460_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOE54258.1"
                     /translation="MPNIKQQIKRVKTNEKARQRNAHFKSMVRTQLKKVRVAIDAKDV
                     ALAEAEYKKAVSLLDKAAGRGVFHRNKVARHKSELARQINELSA"
     gene            7798..8751
                     /gene="fmt"
                     /locus_tag="PK460_03925"
     CDS             7798..8751
                     /gene="fmt"
                     /locus_tag="PK460_03925"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOE54259.1"
                     /translation="MKDLRIIYLGTPDFSAELLRYLIEAGYNIIAVVTQGDSPVGRKR
                     VLTPSPVKTLALAHNIPVFTPLRIRKEYDFIVELEPDLILTFAYGQIVPKAVLEAPKY
                     GCLNFHGSILPKYRGASPIQMALINAEEETGVSLMEMVEAMDAGRVFGIEKFKIEPSD
                     NFLTIASKMVKASATLIEEVLPSVISGENKGVQQEESQVTFAPLLKIKDEWINLENDD
                     VKNVLGRIQAFAPATGALLSYDDLPLKIYEARFDNNNVTRTIGELFISEKKLLLQVKG
                     GQIEVLKLQKSGKKVIDARSFINGEKHKLPTILKGYIDAEI"
     gene            8738..11068
                     /locus_tag="PK460_03930"
     CDS             8738..11068
                     /locus_tag="PK460_03930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOE54260.1"
                     /translation="MRKFNLSVHELVDFVLHSGDLDTRIFNLETMTRGTQIHAFYQAK
                     QNDKYLAEYALEYTFEYENHLITLQGRVDGVIVNGSEVTIEEIKSANTDIDKFFENNE
                     AWHLGQAKCYALMYALRFNLDVIDIQLTYISQIDERKMMKRYTFTREELQSDVFSYFG
                     EYLAFFYQVEKYEKRRTKRLKALNFPYKEVRSSQKVMMEQVEHALEHEHKIFLNAPTG
                     SGKTMAVLYPSLKVLAEKTNTKIFYLTAKGSGKAQAENALRVVREKALVKSTTVSAKE
                     KMCLNDKVACNPDECPYAVNYYGKIRNALLDAFKSRDEYNTEIVTEYAKKHELCPFEF
                     QLDLTLLSDVIIADYNYLFDPFVYLRRFFDSKRGNYIALVDEAHNLPRRVSDNYSLVL
                     DFTPFYSFRKVLKGAEHKKIRRIINRIVKDLDEYKEGIISEYETITEFPHELRSSLDR
                     FLVVGSEYMQEQGNNLAEGFSDFFFDVNKFIKILDLAYEDFILYTEIDEARKFFPKFF
                     IKCLSGAKFINEFLKVIDHAIFFSATLAPAPYFKTMLSIDDDIHQIPNPFSREQLLIM
                     VDGSTSLYYKDREQTLSVVAEKIRAAISVKTGNYIVYTPSFMYLENLEQELRNSEDYD
                     LIVQRPSMSDIEKEKYLETFKLSPTKTTLGLAVLGGSFSEGVDLVSDRLSGVIILGVG
                     YPPPSFEKELEKDYFENKLNDGISYAYVYPALNKILQTMGRVIRSEKDYGFILLMDKR
                     YNYSPYRQQLESYDGLVVQVKSAERISYLLARFFLN"
BASE COUNT         3701 a         1719 c         2156 g         3523 t
ORIGIN      
        1 tttgatttag ggggaaaaat tgatgcgcgt ggtccagaat tattagcaag tgatattgat
       61 aatgacaggt tccttgaaat ttggaatatt gtgtttagtc aatataacgc taaagcggga
      121 gtaaagcgcg agaattacga agaattaccg agtaaaaaca tcgatactgg cgctggttta
      181 gaacggattg ctagtgtatt acaagaagtg ccaagtaact ttgagactga tttatttatg
      241 ccaattatta aagaattaga aaagttatcg ggcgttaaat atagtgatga tatcgttagt
      301 ttccgtgtta ttgctgatca tattcgtaca tcaacattcg caatagcaga tggcgctagt
      361 tttagtaatg aagggcgggg ttatgtctta cgccgtgtat tacgacgcgc ggtgcgttat
      421 ggccaaaagc tcgctatgaa agaacccttc ctttacaaat tagtgcaaag tgtagttgat
      481 gttatgaaag acttctatcc atatttagaa agtaaaatag accatgtcga aaaacttatt
      541 aaagccgaag aagtacgctt tttaaataca cttaatcacg gtgaagaatt attacgcggt
      601 gccctatcaa atagcaagaa catcagcggg gaagttgcat ttaaactcta tgatactttt
      661 gggttcccca ttgatttaac actagaaatc gctagtgaaa atggcgcgag tgtcgatgtg
      721 aaaggctttg aagttttgct tgaagaacaa cgcgaaagag cacgtgctgc gcgtaaaaat
      781 ttaaatagca tggctagtca aagtgaagac ttattaaagt ttgatcagaa gtcaaaattt
      841 gaagaacaaa aatttagtgt taccgcgact gtgattggct tatttaaaga tggtgttaaa
      901 gttaatgaaa gtgcagaaga tgtgagtgcc atctttgatg ttaccccctt ttacagtgaa
      961 agcggcggac aagttagtga tagcggtttt gtaagcaaca aagaacttcg cgctgtcgtc
     1021 agtgcgatga gtaaagcacc acacggtcaa catttacatc atctaacgct tgattacggc
     1081 gtaattaagg ttggtgataa agtacaatta gaaatcgacg gggtagcgcg tcgctccatt
     1141 atgcttaacc acagtgccac acacttatta caccgtgcac ttaacgatgt attaggtagt
     1201 gaagtaagtc aagctgggag ttatgttggt cctgattatt tacgctttga ctttaattat
     1261 aatacccgtg taactgacga agaattagaa aaaattgaat taattgttaa taaatatatt
     1321 agtgactcag tcccattaac tgttaaatat atgactttag cggaagcaca aaagcttggt
     1381 gcaaaagctt tatttatgga aaagtatggt gacttagttc gtgttgttac ttttgccgga
     1441 atatctgttg aactttgtgg tgggacacat attgataata caagtaagat tggtactttt
     1501 gtaattgtaa gtgaaagtag tatcgctagt ggggtgcgtc ggattgttgc tttaaccgga
     1561 ttagcggcct acgaacacct aaaaacatat tccgacactg tgaaaagaat aagtaaagag
     1621 cttgggctta atgataaaaa agatgtcgtg ccacatgtgc gcactctagt agaaaaaaat
     1681 gcccttttaa atagtgaagt cacaatgctt aaaacagctg aactccatcg tactgcttcc
     1741 gctctaacac cgaaaaagca cggtgatacg aatatatatt tagaatattt cactacttca
     1801 aatcgtgaag acttaggagt tattgccgat aaagtgagaa gcactgatga gtgcgcttta
     1861 cttgtgttag ttggtaataa aacaaatgac ttcccactgc ttattagtgt gagtaaagag
     1921 ttatcaaagt cgctaaacgc taataaagta atgcgcgcat taacaagtgc ttttggtgga
     1981 cgcggtggcg gccgacctga ctttgctaac ggtgtgctct taaacttacc agaaaaaaca
     2041 aaagcattag cgacaatatt aaaggtgctt gatgagtaaa atattaggct tagacttagg
     2101 aactaaaacg cttgggatag cgataagtaa tgttgacgcg acatttgcag ttgcttatga
     2161 aaattatgtg tttcccccgc attactataa aaaggcgcgt gcccatgtct tagcaatatg
     2221 cgctaaagaa aacattagtg aaattgcgct tggctacccg cttaacactg atggcactgt
     2281 tggggaaaga gcaaagtctg cggaaagatt ccgtgatgat ctcttgcgtg aaggttcgcc
     2341 gcctattgta ttagttaatg aaacttacac gacgcttgcg gctaccgaag aacttgaacg
     2401 actcggttat aaaaaggagc aaattaaagc acagattgac gctgtcgcgg caaaatacat
     2461 attagaaact tatttagaaa ggaaaagaag caatgaaaga aaatgatgtg attgttatta
     2521 caaccgaaga aggcggagaa gaagagttta ttattctttt tacgtatgaa aatgaagaac
     2581 gaggcacaaa atatgtcttt ttctatgatg aaaataatga tgaagaagtg atgtgcgcga
     2641 agtattttga tgacgggaca ttagaatatg ttgaagacga agaagaactt gcggaaatgg
     2701 aagaagtatt ccgcgcctac gaaagcgaat tagaagagta accgcttccc ttttgttttt
     2761 ggttgagaat tataagcatt atgttataat gcattagcaa tttatatagg agaaatgact
     2821 atgactaata aatatacttt aacccaagaa ggggtcgacg ccttagaaga agaattaagt
     2881 gttctcgtaa atgtagaacg gcctctagtg ctcaaagaaa tcgaagaagc ccgtagtttt
     2941 ggtgacttaa gtgaaaacgc agattacgac gctgcccgta ataaacaagc caatatcgaa
     3001 agccggatta tggaaattga acacatttta gcgcataaag aaatcgttga atattccaaa
     3061 acgagtaaaa cagttgccgt tgggaactat gttgaagttc atgaccgcac aaatgatgat
     3121 actttctcgc tccgtatttt aggacaattt gaaggtaaac cacttaatgg tacagtttct
     3181 gttgatgccc cgattgccca agccatttta ggaaaaaaag ttggtgaccg cgtaaacgta
     3241 aaagcccaac ctccctatga agtggaaatt ttaaatattt cgactgaacc aatcgaataa
     3301 ttaatatttt tgtagtaaaa agataaataa cttcctactt attagtagga ggttttttat
     3361 ttatgttaaa gctaatgatt ggtgtagtga tcgctacagt tattgtcatc tttgccttta
     3421 gtgtgattaa tagcaatatg agcgctaatc cgcaaggaca aagttcaatg catgttattg
     3481 atgataactt tttatcaata acagtgacgg gacaagtcgt aaaaccagga acatatgtga
     3541 tgaaaaaaga cgaaacaatt ggtgacttaa ttatggcagc gggcggacca acaagtaacg
     3601 ctgatgcgcg ctcttatatt gaagacacag tgcttattgc tggtgttagt tattacatcg
     3661 ctccgattaa tgacttagat gattactgtg gggagatgcc gctccataaa gttaatatta
     3721 acgccgaccc caaagataaa ctaatggaag ttaacggcat tggtgagacg ctagctggtg
     3781 cgattattca gtatcgcggg gagcacggcg aatttactta tttagaagaa ctaatgaagg
     3841 taaaaggcat tgccaaagca acatttgaaa aaattaaaaa tcatgtaact ttacgatgaa
     3901 tttattattc atctttctat cattttgctg cggactttta gcatttagac ttccactttt
     3961 tatccttcct ttcttgttgc ttctagtccg cacttttaaa aataaattga cttttacttt
     4021 tgttattgtc ttttttgctt taggtctaat tataatgatt ataagtaact ttattagtaa
     4081 gttacctttt gaaagtagtt ttgggctcat aactaaagcg ggcactaatc atattatagt
     4141 tttaacagtg cgtggaaaat tttatgtgcg gttagaaaat aataaacttg tagaagggga
     4201 cataatttta cttaacggtt ataaagtagc ttactttttt aagcggcttg aaggagaatt
     4261 taattacgcg aaatatttag aaaataaggg aattaaaagt gcgcttaatc tttcgtttta
     4321 tgaactaaaa gtaccgtctt tattacgact atttaataag tttacagtta aaaaaaatac
     4381 ttatcctccg catcttgaac acattaaaaa tttattatta attaaaacaa taaattacga
     4441 tgatcccttt attcatgaat taatacggtt agacttatta tttctaatta gtctaacagg
     4501 agcgcatctc tcgtttttac gccgtgttgt aaagaaaata agcagtgtgt tcgttaacga
     4561 gtttcttcaa gaagtcatca gttacttatg tcttattccg ttatttttac ttaatattac
     4621 taagtttgct ttttatcgta tctttttaac gggaattttg cgtcttatta ataagaagca
     4681 ccttaatgct tactttaata gtgtagaatt aacgtgtatt gctgcttttt tctttttagt
     4741 tattaatcct tattttgttt ttgatcccgc attctactta gcctacttat taacatttct
     4801 ttttcaaatg gtgcgtccgt ctcgttcagt aaaaggaagg ttacaaagct ctcttatcgt
     4861 gatgtgtgtt attgtgcctt ataacatatt tacaagcgga acatttaatt tagtgtatat
     4921 ctttattagt cttttattag tgccgcttat tgctgtgtgg acactagttt actttgtaac
     4981 actttttatt gctcctttgc aaaatgttgg cataaataca cttaatctta tttatcacct
     5041 agttgttttg atgaaaaaaa ttaatatcac actatatttt ggtgatttaa atgtgctttc
     5101 taaactctta ttagttactt ttatcagcgt gttgctttat gcttttaacg ctaagattaa
     5161 accattacaa aaagatatta cgctattaat aacgacattg cttttattaa acttattacc
     5221 acttaatcat tattttgaat atagtattag ttttataaat gtcggtcaag gtgatagtac
     5281 tttactacgg ataaaaaata aatatgtatt aatcgatacg gggggattaa cttataaaga
     5341 tgttggcaat gatattttaa taccctactt aaaacgcaat aaaatttatc gtttagatta
     5401 cttaattatt acacatgatg actttgatca ctgtggtgct aaagaagcgt taatgcataa
     5461 ttttacggtg aaaaatgtta ttaccgaaag acaatatttc ccgcttaatg ttaacggaat
     5521 aacattagag aacttaaata ataagtatta tgatgatgta aatcttgatt cattaatcat
     5581 ctatgtgcct tttccaaagt taaaaatcct tttaatggca gacgccggga taccaaatga
     5641 agtagattta ataaagactt atcctacttt agaggttgac gttttaaagg ttggtcatca
     5701 cggttcaaat acgagtacaa gcgacgactt tattcgtcat tacaagccac aagtagcaat
     5761 tattagctgc ggatatcaaa ataaatacgg tcacccccat cacaccgtac tttcgacttt
     5821 acaaaaatat gatgtgaaaa taaaaagaac agacactctt ggtacaatta ccttaaaaac
     5881 ttgtataata taactatgtt atatctcttt gcggctaatg aatatgcact agtggaaaag
     5941 gtaattcgga aaacggtaga tgctttatta cccgaacgaa atgcttttaa ctatgtgcgt
     6001 tacgacatgc gagaaacgcc ttttagtgaa atcattgaag atgcactaag ttatgctttt
     6061 gatagcagtg tacgtgttat tgttatcgac cacgccagtg tgttaacgac aagtaatgaa
     6121 aaaagtatta ataaagatat tcctgacgaa caaattgatt tgctttcgcg tctaagtgaa
     6181 gatacgcatc ttattttcat cgctcgttct tcacaaatta acccgaaaca taaaattatt
     6241 aagtttattg aaaataacgg gaaaataacg gttgaaaaag atattgataa aaatgattgg
     6301 caacgctttg tgcgtgctta ctttgctaaa gaaaatttac cgattgatga tgaggctgca
     6361 agtgaattac ttagccgcgt cactaatcta ggttcttttt tacaggaagc agaaaaatta
     6421 gcgatttatg gtaaaagagt tactttgcca ctggttgaag aacttgtaac accgctttta
     6481 gaagaaaata gttttgcgct agttaatgcc tttatcagcg gtgacaaagc gaagactatt
     6541 aaaatatatc aagattttaa agtgcaaaac caagaaccag tcgcctttat tgctcaagtc
     6601 ggtaatcagt ttagaacata tgcacaaatt tatatatgtc atgaagaagg gatgacaaac
     6661 gaagaaattg ctagtttttt gaaaattcat ccttaccacg ttaaactagc gatggatcaa
     6721 agacgacgca ctagtttaga agaagtattt aatattttac tcgcgcttgc ggatttagat
     6781 tataaaataa aaagcggcca aattgaccgt ttttatggat ttgaaatgtt tcttcttaat
     6841 tactaagcag aaagttcatt aatttgacgc gctaattcgc tcttatgacg agcaacttta
     6901 ttgcggtgga acacaccacg acctgctgct ttgtctaata aagagacggc ttttttatat
     6961 tcagcttcgg ctaaggcgac atcttttgca tcaatcgcta cgcgaacttt tttaagttga
     7021 gttcgcacca ttgatttaaa atgggcgttg cgttggcgcg ctttttcatt ggtcttaaca
     7081 cgtttaattt gttgcttaat atttggcata ctattacctc tttcaataat aatcttaaat
     7141 attatataaa attataataa ttttagcaaa taaagagtgc aataattttt cagtaattgc
     7201 gtgtttctat tcattatatt cattaaattt cttcaaagta ggcaatagag taatttatag
     7261 cgatactatg attaaaaaaa ctttatttaa agtttaaggc gttttctcct tgcaaaaaag
     7321 tcatgcaagt aataaaaaat accaagtagt aaagatatcc caaatattat aagtatgacg
     7381 acactagcct tcatatcgta agcattccat ccgagtatta gcggaacaga agcatagata
     7441 aaaaagcgcc gtatggataa gcattgtaat gtgaatacaa ggaacgtgta attccttatc
     7501 ttttaaaaat aaaataagta gtgacaatgt aatcattatt gcccctagca catatattaa
     7561 gataactgga tctttaaaaa atatataaat aacgagaatt gtcgtccata acaccattga
     7621 atataaggca aagataattt ttttaattct tttaggcata tgtttatttt tcctagagtt
     7681 caacatatgt tttaaattat agccattaaa gctagtaaat tcaatattat gggacgagga
     7741 tttaatatat ttcattacga agtgtgaatt tttgtctata atttaagcgt gaagagtatg
     7801 aaagatttaa gaattattta tttaggaaca ccagacttca gcgctgaact tttacgctat
     7861 ttaattgaag caggttataa cattatcgcc gttgtcacac aaggtgatag tcctgttggg
     7921 cgcaaaagag tattaacacc aagtcccgta aagactttag cattagcgca taatattccc
     7981 gtttttactc cgcttcgcat tcgtaaagaa tatgatttca tagtggaact agaaccggac
     8041 ttaatcctta cttttgccta tggtcaaatt gtacctaaag cagtactaga agcacctaaa
     8101 tatggttgtt taaattttca cggctcaatt ttgccaaaat atcgcggggc ttctccaatt
     8161 caaatggcac taataaatgc cgaagaagaa acgggtgtta gtcttatgga aatggttgaa
     8221 gcaatggacg ctggccgagt gttcgggatt gaaaaattta aaattgaacc aagcgataac
     8281 ttcctaacaa ttgcaagtaa gatggtaaaa gcaagtgcga cactgattga agaagtttta
     8341 ccaagtgtta ttagcggcga aaataaaggt gtgcagcaag aggaatcaca agttactttt
     8401 gccccgcttt taaagattaa agatgagtgg attaatcttg aaaatgacga tgttaagaat
     8461 gttttagggc ggatacaagc ttttgcgccc gctacaggtg cgttattaag ttacgatgac
     8521 ttaccattaa aaatttacga agcacgcttt gacaataata atgttactcg cactatcggt
     8581 gaacttttca ttagtgagaa aaaactttta ttacaagtaa agggcggaca aattgaagta
     8641 cttaaactac aaaaaagcgg caagaaagta attgatgctc gtagttttat taatggtgaa
     8701 aagcataaat taccgaccat tttaaaagga tatattgatg cggaaattta acctcagtgt
     8761 tcacgagctc gtggactttg ttttacattc tggggattta gatacccgca tttttaattt
     8821 agaaacaatg acgcgaggta cacagattca cgccttctat caagctaaac aaaatgataa
     8881 atatcttgcc gaatatgcac ttgaatatac ttttgaatat gaaaatcatc ttattacatt
     8941 acaaggaaga gtagacgggg ttattgttaa tggaagtgag gtaacgattg aagaaattaa
     9001 aagtgcgaac actgatattg ataaattttt tgaaaataat gaagcatggc atcttggaca
     9061 agcaaagtgt tatgcattga tgtatgcgct ccgttttaac ttagacgtta ttgatattca
     9121 attaacatat atttcacaaa ttgatgaacg caagatgatg aaacgctata catttacgcg
     9181 tgaagaatta caaagtgatg tttttagtta ttttggtgaa tacttagcgt ttttctatca
     9241 agtcgaaaaa tatgaaaagc gccgtacaaa gagacttaaa gcacttaatt ttccttataa
     9301 ggaagtacgt tctagtcaaa aagtaatgat ggaacaagta gagcacgcct tagaacatga
     9361 gcataaaata ttcttaaatg caccaactgg tagcggtaaa actatggcgg tactttatcc
     9421 tagtcttaaa gttttagcgg aaaaaactaa tactaaaatc ttttacttaa ccgcaaaagg
     9481 aagtgggaag gcacaagccg aaaatgcttt acgagtagtg cgcgaaaaag cactagttaa
     9541 aagtactaca gtcagcgcca aagaaaagat gtgtcttaat gataaagttg cctgtaatcc
     9601 tgatgaatgt ccatatgccg ttaattatta cgggaagatt agaaacgcgc tacttgatgc
     9661 ttttaagagt agggacgaat ataacacaga gattgttact gaatatgcca aaaaacatga
     9721 attatgtccg tttgaatttc agttagattt aacacttttg agtgatgtta ttattgctga
     9781 ttacaattat ctattcgatc cttttgtgta tttaaggcgc tttttcgata gtaaacgcgg
     9841 taattatatt gcccttgttg atgaagcgca taacttacca cgccgtgtga gtgataatta
     9901 ctctttagtg cttgatttta cgccgttcta tagttttaga aaagtgttaa aaggcgcgga
     9961 acataagaaa attagacgaa ttattaatcg gattgttaaa gaccttgatg aatataaaga
    10021 ggggattatt agtgagtatg aaacaatcac tgaatttcca catgaattac ggagtagtct
    10081 tgaccgcttt ttagttgtag gtagtgaata tatgcaagaa caaggcaata acctagcgga
    10141 aggctttagc gatttctttt ttgatgtgaa taaatttata aaaattctcg atttggcgta
    10201 tgaagatttt atcctttaca ccgaaattga tgaagcacgg aagtttttcc caaagttttt
    10261 tattaagtgt cttagtggcg caaagtttat taatgaattt ttaaaagtaa ttgaccacgc
    10321 gatatttttt agtgcgacac ttgcaccagc gccttacttt aaaacgatgc ttagtattga
    10381 tgatgatatt catcaaattc ctaacccgtt tagccgtgaa cagcttttaa ttatggttga
    10441 cggtagcact agtctttact ataaagaccg cgaacaaact ctaagtgtag tagcggagaa
    10501 aattcgggca gcgataagtg ttaaaactgg taattatatc gtttatacgc ccagttttat
    10561 gtatcttgaa aatttggagc aagagttaag aaatagcgaa gactatgatt taattgtgca
    10621 acgaccaagt atgagtgata tcgaaaaaga aaagtatcta gaaacattta aactctcgcc
    10681 aacaaaaaca acacttggtc tagcggtatt aggcggctct tttagtgaag gagtcgacct
    10741 tgttagtgac cgcttaagtg gtgtaattat tctaggagta gggtatccgc caccatcttt
    10801 tgaaaaagaa ctagaaaaag attattttga aaataaactt aatgacggaa taagttatgc
    10861 ttatgtttat cctgcgctta ataagatact tcaaactatg ggccgcgtaa ttagaagtga
    10921 aaaagattac ggttttatcc ttttgatgga taagcgttat aattacagtc cctatcgcca
    10981 gcaattagaa agttatgacg gcctagtcgt gcaagtaaaa agtgcggaaa gaatcagtta
    11041 tcttttagcg cgttttttct taaattaact cactattttt aagtgcttat atgaatgtt
//
LOCUS       DAOSLP010000035        10904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_5926_length_10904_cov_9.300581, whole genome shotgun sequence.
ACCESSION   DAOSLP010000035 DAOSLP010000000
VERSION     DAOSLP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5926_length_10904_cov_9.300581"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..491
                     /gene="rplM"
                     /locus_tag="PK460_03935"
     CDS             36..491
                     /gene="rplM"
                     /locus_tag="PK460_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOE54261.1"
                     /translation="MLRQTTHANAATVERKWYVVDATDLPLGRLASEIAMTLMGKNKP
                     EYTPHVDCGDYVIVVNADKVSLSGNKLKTKKYYNTSQRPGGLRVRTAGEMLANYPVEM
                     MERAVWGMVPKGRLGRQIIKKLFVYAGPEHKHSAQQPVELPLRYIGGSK"
     gene            491..889
                     /gene="rpsI"
                     /locus_tag="PK460_03940"
     CDS             491..889
                     /gene="rpsI"
                     /locus_tag="PK460_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOE54262.1"
                     /translation="MAKRKAETYLGTGRRKSSVARIILKPGKGSIIVNDQDVNDYMPY
                     QTLVMDLKQPLTLLDVADKYDVIANVHGGGFTGQAGAIRLGIARALLEAGLDRGTLKR
                     AGMLTRDARAKERKKYGLKGARRAPQFSKR"
     gene            1019..1095
                     /locus_tag="PK460_03945"
     tRNA            1019..1095
                     /locus_tag="PK460_03945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Ile,seq:gat)
     gene            1201..3102
                     /locus_tag="PK460_03950"
     CDS             1201..3102
                     /locus_tag="PK460_03950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HOE54263.1"
                     /translation="MKQELIKNNYYEAVNKEWIDAAVIPGDQPMMSAFLELHLDIEKL
                     LMDLAKKWDKDQTGLNNHLQEFVKLYKMTNDFATREKLGAEPLKPVIKKILNLKSLKD
                     LEKNMKDFVLDGVDLPFSFMVFQDFKDSNNEVLYFTAANLFLPDTSYYENEETKQQLI
                     GLFTMTTMQVLSLYGLEEEAAQDLLNKALAFDAMLVPVTMSSVERADMIKMYNPLLKD
                     EFASKTKTFNLLKVAEALVGQDVDKVVAMNLDFIDKFEEIVNDANFELIKAWMVVSNI
                     LTFSAELTEELRVAGGAFGRALQGIAEAQSQEKAAFYQAYDRLSQAVGLYYGLTYFGP
                     EAKKDVEDMVNTIIGVYKDRINKNTWLNAETKAKAILKLNTMKVHIGYPRELPKYYDK
                     YKVGSYAEGSNLVKEKLKYSRLVQKENFKKYNKKPNRNLWAMPASMVNAYYQPMNNQI
                     VFPAAILQEPYYSLSQGRSANYGGIGAVIAHEISHAFDNNGAKFDEYGSLNNWWTDED
                     LKTFEEKAQKMIALFDGVESGYGPCNGTLTVSENIADSGGIRCALEASKKEKDHNYEE
                     FFKNWARVWRQKSSPEFMQLLLTVDVHSPSNLRANMQLKNLVEFQEFYNLTEQDEMYL
                     AKEQMVEIW"
     gene            3197..3272
                     /locus_tag="PK460_03955"
     tRNA            3197..3272
                     /locus_tag="PK460_03955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3230..3232,aa:Val,seq:cac)
     gene            3283..3358
                     /locus_tag="PK460_03960"
     tRNA            3283..3358
                     /locus_tag="PK460_03960"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3316..3318,aa:Thr,seq:tgt)
     gene            3365..3449
                     /locus_tag="PK460_03965"
     tRNA            3365..3449
                     /locus_tag="PK460_03965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3399..3401,aa:Leu,seq:tag)
     gene            3557..3748
                     /locus_tag="PK460_03970"
     CDS             3557..3748
                     /locus_tag="PK460_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE54264.1"
                     /translation="MINYAQKIREYRERKFLTQTEFAALLGVSIASVTRWETGHYQPT
                     MKVKKKIYNLLVEAGIEIE"
     gene            3924..4778
                     /locus_tag="PK460_03975"
                     /pseudo
     CDS             3924..4778
                     /locus_tag="PK460_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214884.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
     gene            4936..5445
                     /locus_tag="PK460_03980"
     CDS             4936..5445
                     /locus_tag="PK460_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE54265.1"
                     /translation="MCQKTFEEKLAELDACIEKHQKSPGPLMPTLHDAQEIFGAIPLE
                     IQKHISKKLGVSVAHINGVVTFYSRFSLTPKGKYIISVCMGTACYVKGAQAILDAVQS
                     ELKLKPGETSEDGLFTLEATRCIGACALAPVYSINDEIYGGGTSKKILEDLRKIIKAE
                     EEKNDQNKA"
     gene            5426..5791
                     /locus_tag="PK460_03985"
     CDS             5426..5791
                     /locus_tag="PK460_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54266.1"
                     /translation="MIKTKRDLEVIKKRMQPDFHLRKEEHKYRLLFRYNEKTVIQNIE
                     KIIDLCLAAIYQDKLDAVAIKVYDTRVEDKLILVVKEKNRAVRYNIKNEAMVTEILNK
                     HIGQGKVVYEYIDVMDKEA"
     gene            5794..7587
                     /locus_tag="PK460_03990"
     CDS             5794..7587
                     /locus_tag="PK460_03990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOE54267.1"
                     /translation="MHKHYEIVLCGGSGCVSSKSQLIKQKFEEILARENLSDFVKVYM
                     TGCFGMCEKGPVVVVYPDQTFYAKVKITDVEEIVESHIKKGLIVDRICHHEFDEKLQK
                     MVYKNLHEMKFHSAQKRLALRNCGVIDPDEIDEYIGRDGYFALEKALRMEPKEIIQLI
                     TDSGLRGRGGGGFPTGLKWQLAERFRGGQSYVICNADEGDPGAFMDRSILEGDSHAVI
                     EAMIINGYAIGANQGFVYIRAEYPLAVKRLQNAINQAYKYGLLGKNIMNSGFDFDLEI
                     RLGAGAFVCGEETALISSIEGGRGMPRKKPPFPAEHGLWGKPTIINNVETYANIPQII
                     LNGPEWFQKIGTERSKGTKVFALGGKINITGLIEVPMGTTLREVIYDIGGGIPNGKKF
                     KAVQTGGPSGGCLTEEDLDTPIDYDTLIQKGSMMGSGGIIVMDEDNCMVDVAKFYLDF
                     IVDESCGKCTPCREGTRRLYELLEKITEGKGTLETLEELKQLCYDVKDTSLCGLGQTA
                     PNPILSTFNKFYDEYVEHVVDKKCRAGVCKSLTTLYITDKCIGCTKCVRNCPVNCITG
                     ELRKLHRIDTTKCIKCGACIQNCPVHAIIHV"
     gene            7602..9365
                     /locus_tag="PK460_03995"
     CDS             7602..9365
                     /locus_tag="PK460_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOE54268.1"
                     /translation="MKKVNITIDNKKLEVDSNITVLQAATDADINIPRLCFLKGISET
                     SSCRICVVEDVGRNRLVNSCTYKVYEGLNILTTSPRVIKQIKINLELIAGNHQFDCWA
                     CSREHNCELLELLRRFNIDNHYNINKEIKKREWIVNKSESMVLDSSKCILCGRCIDAC
                     NHYTGLGILGFNNRGFMTSVGPAGGVNIGEAGCIYCGKCIQSCPTGALREKDDVDRAF
                     DLLDEEGAYVVAQIAPSVRATLGEEFGYPMGTNVEGKIYHALEKLGFHDVVDVNFAAD
                     LTIMEEGTELIGRLKAFLDGDKDVKLPLFTSCSPGWIRYIERYAPEYLGHVSSCKSPQ
                     GMSGAIIKNYYAQKLGIEREKIKVVSIMPCIAKKYEASRPEMEVDGVRDIDLVLTTRE
                     LARFIKRHVIDFDMLTNKQLTSPLASYTGAGVIFGVTGGVMEAALRTVKALLEKEDAY
                     LIDIREVRGFESIKEATLTIDGKELNFAVVHGAKHFKEVLELVKSGKKQYAFIEFMGC
                     HGGCIFGGGQPITKAKDFEKIDVLKERAKALYRIDQSGKIRRSHENPHVLKLYEEFLG
                     HPGSHLAHKLLHTTYSKKDVY"
     gene            9366..10259
                     /locus_tag="PK460_04000"
     CDS             9366..10259
                     /locus_tag="PK460_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54269.1"
                     /translation="MKKKIATLLLTSLLLGCQKQTVKLSLLVPAGSPLLTVAGVTSEL
                     EKEVTVGSDLIPAAFTKGEKDLIAAPIIIGAKLFNAGKSNYQLAAMLGWGNLHILSRT
                     LINTIDDLSGKSGLAFAEHSTPGIMFKLATSDVSDINVNYVKAVSDVGGPFIQKQYDY
                     VLISEPVLSKIRSNINDETYTFSLQNVAKLPKIAQFGLFSNPATKAKDVNKFLVKLKN
                     NVEQLNTDPESYVDSIIDSDDYFKGLGRDVLIDAIPRLDIEYKSALEAKADLTLFFDY
                     LISVDTNLIGATSLDESFFRS"
     gene            10237..>10904
                     /locus_tag="PK460_04005"
     CDS             10237..>10904
                     /locus_tag="PK460_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54270.1"
                     /translation="MKASSVPKKKPTAQIIASSLGFLTIILLWIIISALHQNDIIFPG
                     IISTLKVFFSLFQRGPTYFILLNTIGGLILVLVLSFLTALILSLISLKFPTFKSYITP
                     FLALFKIIPVPALLIVFLVNFKQSFIPFLLTYIVLLPLVYDGLYGAFISINKDLIDEL
                     KITSKLNLLMIFKIYIPIVSKQVISTLLQALGLGLKVKVMSEFIAEAPNTIGYELSYG
                     RATLS"
BASE COUNT         3732 a         1672 c         2242 g         3258 t
ORIGIN      
        1 ggtaagacga aaattggtaa aggagaaata caaatatgct acgtcaaaca acacatgcga
       61 atgctgcaac cgttgaacgt aaatggtatg tcgttgacgc tactgatctt ccactcggtc
      121 ggttagcgag cgaaatcgcc atgacgctca tgggcaaaaa caaaccagaa tataccccac
      181 atgttgattg tggagattat gttatcgttg ttaacgctga caaggttagt ttaagcggca
      241 ataagctcaa aacaaagaaa tattacaaca ccagccaacg tcctggcggt ttacgcgtgc
      301 ggacagcagg tgaaatgctc gcaaattatc ctgtcgaaat gatggaaaga gcagtgtggg
      361 gcatggttcc aaaaggtcgt cttggccgtc aaattattaa gaaattattt gtctatgctg
      421 gaccagaaca caaacatagt gcacaacaac cagtggaact acccttacgt tatattggag
      481 gaagtaaata atggcaaaga gaaaagcaga aacttactta ggtactggcc gtcgtaaatc
      541 aagcgtcgca cgcattattt tgaaacctgg taaaggttca attattgtta atgatcaaga
      601 tgttaatgat tacatgcctt atcaaacctt agttatggac ttaaaacaac cattaacttt
      661 acttgatgtt gccgacaaat atgatgtcat cgctaacgtt catggtggtg gctttaccgg
      721 tcaagcaggg gcaatccgtt taggaattgc gcgtgcttta cttgaagctg gccttgatcg
      781 cggtacactt aaacgcgcgg gaatgttaac ccgtgatgct cgtgccaaag aacggaagaa
      841 atacggtctt aaaggcgctc gtcgtgcacc acaattctcc aaacgttaat ataactacac
      901 gagaacaaac gaaagtctgg ttcaaaatgc cagacttttt cttatattaa aaacttttaa
      961 tgttaaaaaa gttgcaatat gattaatgtt gttatattat atattagcgt ccgcagatgg
     1021 gcccttagct cagttggtta gagcgcaccc ctgataaggg tgaggtcgat agttcaaatc
     1081 tattagggcc caccatttgc aagtaaagtc actactttgt agtgattttt ttgtaaagaa
     1141 taagtaaact tcctctattt accatacata aatgttaaaa tacgatggtg aggtgaaaat
     1201 atgaaacaag aactaattaa aaacaattat tacgaagctg ttaataaaga atggattgat
     1261 gccgcagtaa ttcctggtga tcaaccaatg atgtcagcat ttttagaact acatttagat
     1321 attgaaaagt tattaatgga tttagcgaaa aaatgggata aagaccaaac aggattaaat
     1381 aatcatctgc aagaattcgt taagctttac aaaatgacga atgattttgc tacaagagaa
     1441 aagctcggtg ctgaaccact taaaccagta attaagaaaa tcttaaattt aaagtcactt
     1501 aaagacttag aaaaaaatat gaaagatttt gtgttagatg gagtggattt accatttagc
     1561 tttatggtgt tccaagattt taaagattca aataacgagg tgctatattt taccgctgcg
     1621 aatttattct taccagatac ttcatattat gaaaacgaag aaacgaaaca acaacttatt
     1681 gggttattta caatgacgac aatgcaagtg ctaagtttat atggtttaga agaagaagcg
     1741 gcacaagact tattaaataa agcgttagcg tttgacgcga tgctagtgcc agtgacgatg
     1801 tcgagtgttg aaagagccga catgattaaa atgtataacc cattattaaa agatgaattt
     1861 gccagtaaaa caaaaacatt taatttactc aaagttgcgg aagcattagt cgggcaagat
     1921 gttgataaag tagtagcaat gaatttagac tttattgata agtttgaaga aattgttaat
     1981 gacgctaatt ttgaactaat taaagcgtgg atggttgtct ctaacatcct tacattcagc
     2041 gctgaattaa cggaagaatt acgggttgct ggtggtgctt ttggccgcgc tttacaaggt
     2101 attgcggaag cacaatcaca agaaaaagca gcgttttatc aagcttatga ccgtctttca
     2161 caagctgttg ggctctatta cggcttaact tactttgggc cagaagctaa aaaagatgtt
     2221 gaagatatgg ttaatactat tattggtgtt tataaagacc ggattaataa aaacacatgg
     2281 cttaacgctg aaacaaaagc aaaagcaatt cttaaactta acaccatgaa agtacatatt
     2341 ggttatccgc gtgaattacc aaaatattat gataaataca aagttggcag ttacgctgaa
     2401 gggtcaaatt tagtgaaaga aaaacttaaa tatagccgtt tagtgcaaaa agaaaacttt
     2461 aagaaatata ataaaaaacc aaatcgtaat ttatgggcaa tgccagcttc aatggttaat
     2521 gcttactatc aaccaatgaa caatcaaatc gttttccctg ctgcgatttt acaagaacca
     2581 tattatagtt taagtcaagg tcgtagcgct aattacggcg gaattggtgc agttattgcc
     2641 catgaaatct cgcacgcctt tgataataat ggcgcaaagt ttgatgaata cggctcactt
     2701 aataactggt ggacagatga agatcttaaa acatttgaag aaaaagcaca aaaaatgatt
     2761 gcattatttg atggtgttga atcaggatac gggccgtgta atggtacttt aaccgtttcc
     2821 gaaaacatcg ctgatagtgg cggaatccgc tgtgccttag aagcaagcaa aaaagaaaaa
     2881 gatcataatt atgaagaatt ctttaaaaat tgggcgcgtg tctggcggca aaaatctagt
     2941 ccagaattta tgcaattact cttaactgtt gatgtgcata gtccatcaaa cttacgtgca
     3001 aatatgcaac ttaaaaacct cgttgaattc caagagtttt ataatttaac ggaacaagac
     3061 gaaatgtatt tagcaaaaga gcaaatggtt gaaatttggt aaacaatcaa atcttgtaaa
     3121 ctaagagggc ttgtccctct tttttaattg ctatttataa atgattttgc tattatatta
     3181 caaggtcgtc ccttccggac acttagctca gatgggagag cgtttccttc acacggaaga
     3241 ggtcgggggt tcgatcccct cagtgtccac catttattta acgccgacgt ggctcaattg
     3301 gcagagcaac tgatttgtaa tcagtaggtt gtaggttcga gtcctatcgt cggcaccatt
     3361 acttgcaaac gtggcggaac tggcagacgc gctagactta ggatctagtg gggaaaccca
     3421 tgcaggttca attcctgtcg tttgcaccac gttacttaaa ttgctcctta aacggggcat
     3481 tttttattaa ttccaatagc gttagtgata aaaaagtgat aaaataatgt gtaaatagtt
     3541 taaataggag tttatcatga ttaattatgc ccaaaagata agagagtatc gtgaacgaaa
     3601 gtttttgaca caaacagaat tcgcggcgct tcttggtgtt tctatcgcat cagtgacgag
     3661 atgggaaaca ggacattatc agccaacaat gaaagttaag aaaaaaatat acaatttatt
     3721 agttgaggct ggaattgaaa tagaataaaa ggagattaac tattagaggt taagaaaaaa
     3781 gataagagta tagtaaaaaa actccacaag gctgtaggta aaattaacaa gtaaatagtg
     3841 taattgttat acccggagaa agaggagaat attatctaat cattaattaa accgggtagg
     3901 attagataat atgaaattaa gttttgaaaa acgaaattaa attatttgaa ggcaaacaaa
     3961 ttagaacaca ttgggatgaa gaaaaagaag aatagtattt tagtgttgta gatatcattg
     4021 gcgtcttaac agagagcaaa gaccccggag catactggag aaagctaaaa cagcgattaa
     4081 aagaagacgg aagtgaaacc gtgacatttt gtcacgcttt gaaattgaaa gcatcagatg
     4141 ggaaaatgcg taacactgat atcgctgata tacgaggaat ttttcgcatc atacaatctg
     4201 ttccatcgcc taaagccgag cctttcaaga tgtggttaac ggaagtagga aaagagcggg
     4261 ttgatgaaat aatggatcca gagctaacta ttgatcgtgc tttacgcaca tatttagcaa
     4321 aaggttacac ccgtgaatgg attaatcaaa gattacaagc aatatcggta cgtaaagagt
     4381 taacagatgc gtggcaagat catggtgtta aagctggttc agaatacgca atattaagaa
     4441 atgaaattac aaaagcctgg agtggaatga caactaggca atataaaaat ctaaaaggtt
     4501 taacaaaaga aagtttacga gataatatgt caacaacaga aattgtgtta aacatgcttg
     4561 cagaaacggc cactaaagaa attattaatg taaagcatcc tgatggactt gaagaaaata
     4621 tcgagatagc aaatgagggt gggagtgttg ccaaagcggc gcgcgaagca attgagtcaa
     4681 aaactggaaa aaaagttata acgagtaaaa aagcacttga tttaggaaga gtaattaccg
     4741 ctataaccga aaaaaatgat gatgataatg aaaaataata agataatgcg ttgtaattga
     4801 catgtacgct ttttaagaat atctaagatt tttcaatgtc tcattaaaaa aacatttata
     4861 atattgtttt gaatttataa tattaatatt ataatcatag ttgtatctgg aaataaatac
     4921 cgataggaga cgaatatgtg ccaaaaaaca tttgaagaaa aacttgcgga acttgatgct
     4981 tgtattgaaa agcatcaaaa gagtccaggg ccactgatgc ccacattaca tgatgctcaa
     5041 gaaatatttg gagcaattcc cttagaaatt caaaagcata ttagtaagaa attaggcgtg
     5101 agtgtcgccc acattaatgg tgttgtgaca ttttactcac gtttttcttt aaccccgaaa
     5161 ggaaaataca ttatttcagt ttgtatgggg acagcgtgtt atgttaaagg cgcgcaagct
     5221 atacttgacg ctgtgcaaag tgaattaaaa cttaaaccag gcgaaactag tgaagacggt
     5281 ttatttacgc ttgaagcaac gcgctgtatt ggtgcatgtg cgcttgcccc tgtttattca
     5341 attaatgatg aaatttatgg cggtgggaca agtaagaaaa ttcttgaaga cttacgaaaa
     5401 ataattaaag cagaggaaga aaaaaatgat caaaacaaag cgtgatttag aagtaattaa
     5461 gaaaagaatg caaccagatt ttcatttacg gaaagaagaa cataaatatc ggttattgtt
     5521 tcgttataat gaaaaaacag taattcaaaa tattgaaaaa ataattgatc tctgtttagc
     5581 ggctatttat caagataaac ttgatgcagt agcaattaaa gtttatgata cacgcgttga
     5641 agataaattg attttagttg ttaaagagaa aaatcgtgct gttcgttaca acattaaaaa
     5701 tgaagcgatg gttactgaaa tattaaataa acatattggt caaggtaaag tcgtatatga
     5761 atacattgac gtgatggata aggaggctta attatgcata aacattatga aattgtcctt
     5821 tgtggtggtt ctggatgtgt ttcctcaaag agtcaattaa ttaaacaaaa atttgaagaa
     5881 atccttgccc gcgaaaactt aagcgatttt gtaaaggttt atatgacagg atgctttggc
     5941 atgtgtgaaa aaggaccagt tgttgtcgtt tatcctgatc aaacatttta cgcgaaagta
     6001 aaaattactg atgttgaaga aatagtggaa agccatatta aaaaaggttt aattgttgat
     6061 cgtatttgtc atcatgaatt tgatgaaaaa ttgcaaaaaa tggtgtataa aaatttacat
     6121 gaaatgaaat tccatagtgc ccaaaaacgt ttagcgctcc gtaactgtgg ggtaattgat
     6181 cctgatgaaa ttgatgaata cattgggcgt gatggctatt ttgcccttga aaaagcttta
     6241 cggatggagc ctaaagaaat tatccaatta attactgatt caggcttaag agggcgcggt
     6301 gggggcgggt tccccacggg gttaaaatgg caacttgcgg aacggttccg tggtggacaa
     6361 agttatgtta tttgtaacgc tgacgaaggt gacccaggtg cctttatgga tcgctcaatt
     6421 ttagaaggtg atagtcacgc tgtgattgaa gcaatgatta ttaatggtta cgctattggg
     6481 gcaaatcaag gatttgttta tattcgcgct gaatatcctt tagcggttaa acggctacaa
     6541 aatgcgatta atcaagctta taaatacggt ttattaggca aaaatatcat gaatagcggg
     6601 tttgattttg atttagaaat tagacttggc gctggtgctt ttgtctgtgg tgaagaaacg
     6661 gcccttattt catctattga aggtggacgc gggatgccgc gaaaaaaacc gccattccct
     6721 gctgaacacg ggttatgggg aaaacctaca attattaata atgtggaaac ttacgctaat
     6781 attccgcaaa ttattttaaa tggtccagag tggttccaaa agattggcac tgagcgttct
     6841 aaaggtacaa aagtgtttgc cttaggcggt aaaattaata ttactgggct cattgaagta
     6901 ccgatgggca caacattacg cgaagttatt tatgatattg gtggcggaat tcctaatggc
     6961 aagaaattta aggctgtcca aactggcgga ccaagtggtg gctgtttaac tgaagaagat
     7021 ttagatacac caattgatta cgatacatta atccaaaaag gttcaatgat gggttccggc
     7081 ggtattatcg tcatggacga agataactgt atggttgacg tcgctaagtt ttatcttgac
     7141 tttattgttg atgaaagttg tggtaagtgt acaccgtgtc gcgaaggcac aagacgtctt
     7201 tatgaacttt tagaaaaaat tactgaaggt aaaggtacac tcgaaacact tgaagaatta
     7261 aaacaacttt gttacgacgt taaagatact tcgctttgtg gtttaggtca aaccgcgcca
     7321 aatccgattc ttagtacgtt taataagttt tatgatgaat atgttgaaca cgtcgttgat
     7381 aaaaaatgtc gcgctggtgt atgtaaatca ttaacgacac tttatattac cgataaatgt
     7441 atcggttgta cgaagtgtgt gcgcaattgt ccagttaact gtatcactgg cgaattacgg
     7501 aagttacacc ggattgacac aacaaaatgt attaaatgtg gtgcgtgtat tcaaaattgt
     7561 ccagtacacg ccatcattca tgtatagagg taatgattat gattaaaaaa gtaaatatta
     7621 caattgataa taaaaaatta gaagttgata gtaatatcac tgttttacaa gcggctactg
     7681 atgcggacat taatattccg cgcctttgtt tccttaaagg tatcagtgaa acttcatcat
     7741 gccggatttg tgttgtcgaa gacgttggtc gtaatcggct tgttaactca tgtacttata
     7801 aagtatatga aggactaaat atcttaacaa cttcaccacg ggttattaaa caaattaaaa
     7861 ttaacttaga actaattgct ggtaaccacc aatttgattg ttgggcatgt tcacgggaac
     7921 acaactgtga attacttgaa ttattacggc gctttaatat tgataatcat tacaacatta
     7981 ataaagaaat taaaaaacgt gaatggattg ttaataaaag cgaatcaatg gtgcttgata
     8041 gtagtaaatg tattttatgt gggcgctgta ttgatgcctg taatcattac actggattag
     8101 gtatccttgg ttttaataac cgcggattta tgacaagtgt tggtcctgct ggtggtgtga
     8161 atattggtga ggctggttgt atatattgtg gtaaatgtat tcaaagttgt ccaacaggag
     8221 cattacgcga aaaagatgat gttgatcgcg cttttgactt acttgatgaa gaaggcgctt
     8281 atgttgttgc gcaaattgct ccaagtgtta gagcaacatt aggtgaagaa tttggttacc
     8341 caatgggtac taatgtcgaa ggtaaaattt accacgcatt agaaaaactt gggttccatg
     8401 atgtagtgga tgttaacttt gccgctgact taacaattat ggaagaaggc actgaattaa
     8461 ttgggcgttt aaaagcattc cttgacggtg ataaagatgt gaagttacca ctttttacaa
     8521 gctgttcacc aggctggatt cgttatattg aacgctatgc tcctgaatac ttaggacatg
     8581 tttcttcatg taaatcacca caaggcatga gcggagcgat tattaaaaat tattatgcgc
     8641 aaaaattagg gatagaacgc gaaaagatta aagtcgtgtc aatcatgccg tgtattgcta
     8701 aaaaatacga agcaagtcgt ccagaaatgg aagttgatgg tgttcgtgat attgatttag
     8761 tactcacaac tcgtgaatta gcgcgtttta ttaaacgcca tgtcattgat tttgatatgt
     8821 taacaaataa acaattaaca agcccgcttg cctcttacac tggcgctggt gttatttttg
     8881 gcgtaacagg tggcgttatg gaagcagcat tacgtactgt taaagcatta cttgaaaaag
     8941 aagatgctta tttaatcgat attcgtgaag tacgcggctt tgaaagtatt aaagaagcaa
     9001 ccttaacaat tgatggtaaa gaacttaact ttgcggttgt gcacggcgct aaacatttta
     9061 aagaagtgct tgaactagta aaaagtggta aaaagcaata tgcctttatc gaatttatgg
     9121 ggtgccatgg cggctgtatc tttggtggag gtcaaccgat tactaaagct aaagactttg
     9181 aaaagattga tgtattgaaa gagcgcgcta aagcactcta ccgcatcgac caaagtggaa
     9241 aaatccgccg ttcacatgaa aatccacatg tcttaaaatt atatgaagaa tttttaggcc
     9301 atcctggctc acatttagcg cataaactat tacatacaac atatagtaaa aaggatgtgt
     9361 attaaatgaa aaagaaaatt gcgactttgt tattaactag cttactttta ggttgtcaaa
     9421 aacagactgt taaattatct ttattagtgc ctgctggcag cccgttatta acagttgcag
     9481 gtgtaacaag tgaattagaa aaagaagtga cagttggatc cgacttaatt ccggcagcgt
     9541 ttactaaagg tgagaaagat ttaatcgctg caccgattat tattggcgca aagttattca
     9601 acgctggtaa atcgaactat caactcgccg caatgctcgg ctggggtaac ttacatattt
     9661 taagtcggac actaattaat actattgatg acttaagcgg gaaaagtggt ttagcttttg
     9721 ctgaacattc aacaccaggc attatgttta aattagcgac aagtgatgtt agtgatatta
     9781 atgtaaatta tgttaaagca gttagtgatg tcggcggacc atttatccaa aaacaatatg
     9841 attatgtttt aattagtgaa ccagttttaa gtaagattag aagtaatata aatgatgaaa
     9901 catatacatt ttccctgcaa aacgttgcta aattacctaa aatagcgcag tttggattat
     9961 ttagtaatcc cgctactaaa gcaaaagatg taaataaatt tttagttaaa cttaaaaaca
    10021 atgttgaaca acttaacact gatccagaaa gttatgttga tagtattatt gacagtgatg
    10081 actactttaa gggactcggt cgagatgttt taattgatgc tattccccgt ttagatattg
    10141 aatacaaaag tgcgcttgaa gcaaaagcag atcttactct cttttttgat tatttaatta
    10201 gtgttgacac taacttaata ggagcgacga gtcttgatga aagcttcttc cgttcctaag
    10261 aaaaaaccaa cagcgcaaat tattgctagc tcattaggct ttttgacaat cattttacta
    10321 tggattatta ttagtgcgct gcaccaaaat gatattatat tccccggaat tattagtact
    10381 ttaaaagtgt ttttttcttt attccaaaga gggcctacat attttatttt actaaacact
    10441 attgggggat taattctcgt tttagtttta tcatttttaa cggcattaat tttaagttta
    10501 atatcgttaa aatttccaac ttttaaaagt tatattacac cctttttagc actatttaaa
    10561 attatccctg ttccagcgct tttaattgtg tttttagtta attttaaaca aagctttatt
    10621 ccgtttttac taacatatat tgtgctttta ccacttgttt atgatggatt atatggggct
    10681 tttattagca ttaataaaga tttgattgat gaattaaaaa ttacttcaaa acttaattta
    10741 ttaatgattt ttaaaatata cattccgatt gtaagcaaac aagttatttc gactttatta
    10801 caagccttag ggctaggact taaagtaaaa gttatgagcg aatttatcgc tgaagcgcct
    10861 aatacgattg gttacgaact ctcttatgga cgggcaactt tatc
//
LOCUS       DAOSLP010000036        10689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_6071_length_10689_cov_10.283242, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000036 DAOSLP010000000
VERSION     DAOSLP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6071_length_10689_cov_10.283242"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..335)
                     /locus_tag="PK460_04010"
     CDS             complement(114..335)
                     /locus_tag="PK460_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE54271.1"
                     /translation="MEINEAFGIRLRQLRKAKGWSLLELSVRANVNHNYLCDLENGRR
                     NPTLALLYRISEAFGITLSSLLLGIELIQ"
     gene            complement(322..1299)
                     /gene="whiA"
                     /locus_tag="PK460_04015"
     CDS             complement(322..1299)
                     /gene="whiA"
                     /locus_tag="PK460_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOE54272.1"
                     /translation="MKNVSFTTRAKEDITFNTISYSLPRRKATLAALIKHAGHLIIRE
                     KKEVLKLSTENAKVAKYIYQLFKDIFNFEPTFSYVTKRKFGKKMKFNVIVEQKVDEII
                     ETLAVDFYDSAVSRKIVYSDDTLGGYFTGAFLGSGSMTSPLSSNYHLEIVVRQKKYAE
                     HLARMLNRFEKTNFNAKVTARRDKYIVYLKRSDQISSFLVFVGAANASLEFEGIRMDR
                     DMQNITNRMFICDEANYNKTVKSATEQIENIKIVQRVYGHTPHNNEKLGALMRLRLEN
                     EDATMNELAEMLTEELGSEKRITKSNVNHLFIKLRELAEPFKELSRGNK"
     gene            complement(1359..2282)
                     /gene="trxB"
                     /locus_tag="PK460_04020"
     CDS             complement(1359..2282)
                     /gene="trxB"
                     /locus_tag="PK460_04020"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOE54273.1"
                     /translation="MNKEIIPILIIGAGPAGMTAAIYLSRANATFKIVDKYAPGGKMN
                     ITQDVENYPGIETISGPDLAFKMFEQLRSLGIEVGYADVNKITKEDDYFVIHSDEETL
                     YAKAVIVATGTQERKLKIPGEARYTGKGVSYCAICDGNLYKNDAIMIIGAGNSAIEEA
                     LFVSKIVKHVHIVHRNERFRADESLVNELKAMPNVTFHLNYIPVEILGDTKGVTSVAI
                     KNVIDGTDKVIEVKAVFPFVGSDASADFLSEFDVRDDKGFVLVNEEMMTSVPGLFSIG
                     DVNQKVLRQIVTATNDGAIAALAANRFVKRK"
     gene            complement(2275..2922)
                     /locus_tag="PK460_04025"
     CDS             complement(2275..2922)
                     /locus_tag="PK460_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE54274.1"
                     /translation="MKSKYDLVVLDLDGTIADSDLGLVKIGIKMSETYLPRKDINMDD
                     YLFLNGPTLDETMPYLFPGYPLEELIAKYNEYLPDSVKDITLFKGAKELIKTLKNNNI
                     ELALLTNRARASTEQILKRHGVHSYFKTLVCVDDGFKKKPSGEGLVHIMKTLKSAPDK
                     TLFVGDNWRDILAGQDAGVDVAFIHQHRRPHRLDVSATYVLDDISDVLGVVQSDE"
     gene            complement(2912..4222)
                     /gene="lgt"
                     /locus_tag="PK460_04030"
     CDS             complement(2912..4222)
                     /gene="lgt"
                     /locus_tag="PK460_04030"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOE54275.1"
                     /translation="MDLTLVVVVIFGLIFIGAVTVLGLSLNEFVKKEEDINTLFKGKH
                     RLIAISVLSGAVSVLMLFLPLMVLSNSVLHSLLIGLGSFLFALMLLTFIAAFVLHYYK
                     FNVLQREWIKESKIVTIISGVLSIVFLFVLLEGLTLAEIIKFPLPRGIPFGDSPVIAF
                     YAIFILTGALLVLAITDHEFYKKYGRHGILENVFYVAFPAGIIGARIWYVIGEWNNPE
                     SGFAENPLTIFAIRDGGLAIMGGALFGIIAGVWFFVKRRKAYDIGFAADIIIPTILVA
                     QAIGRWGNFFNQEVYGGDITDISKWWFLPEFIKRQMFILGKYRQPFFLIESALNLTGY
                     FVIRYGVGEGLKKYRKPFDMAFMYIVWYGLVRFIMEPLRNPMFRMGAGGKWSEYNALI
                     FFVVGVALIVLNHIFDFHKLLTRKKGTAEVVSNEPSESVEKNEE"
     gene            complement(4224..7064)
                     /gene="uvrA"
                     /locus_tag="PK460_04035"
     CDS             complement(4224..7064)
                     /gene="uvrA"
                     /locus_tag="PK460_04035"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOE54276.1"
                     /translation="MDKKDYIIIKGARDNNLKNISVNIPKNKLVVFTGLSGSGKSTLA
                     LNTIYNEGQRRYVESLSAYARQFLGNLAKPDVDSIEGLSPAISIEQKSTSNNPRSTVG
                     TITEIYDYLRLLYARIGMPYCPQHDIPISSSSPEQILNKIFEYPEKTRIQILAPLVKA
                     EKGTHKDTLAKIAKEGFVRIRINGEIKALEEVGNLDKNKRHNIDLVINRVVLKAENKS
                     RIFEGINTALDYGHGYVIILFNNEETLFSTHNACHICGFSVPRLEPRLFSFNAPMGFC
                     PECRGLGLKREVDINILVPDKIVSLAKGAIEYYKNIVGTKNIEWQEFEKLCELYNIPM
                     HVPFKELSDKHKEIALFGSPNEHNFKIITGSGNTMYRRGKIEGVKNKIERLYEETTSE
                     FMRQYYEKFMRDVTCSTCNGKRLNEHALAVYINGKNIHDLTMLQIGELKQFLNETRPK
                     LSVTDRTISQLVLDEIDHRLTFLVKVGLDYLTLARSAATLSGGEAQRIRLATQIGSKL
                     TGVLYVLDEPSIGLHQRDNNKLIQALRDMVDLGNTLIVVEHDEETIRSADHIIDIGPG
                     AGIHGGEVVVTGTVEDVINCKKSITGDYLSGRKVIPLPPRRMPGNGNYLKIYGAAANN
                     LRHIDVSIPLNTLTLVTGVSGSGKSSLVEEVLFKAVDGHLRRVETYPGAYSKIEGLEH
                     IDKVINIDQDPIGRTPRSNPATYTKVFDDIRELFAQTVDARARGYTKSQFSFNTKGGR
                     CEHCEGAGVTRIAMNFLPDVYVKCEMCDGQRYHADTLSIHYRGKNIVDVLNMTVEEAL
                     EFFANRPQIVRRLQTLYDIGLGYIKLGQPAPELSGGEAQRLKLAFELQKRPTGKTLYL
                     LDEPSTGLHADDVGRLITSLQAIVANGDTVIVIEHNLDIIKVADYIIDLGPGGGNYGG
                     RVVASGTPEEIVKVKGSYTGEYLAPYLKKEKK"
     gene            complement(7177..7446)
                     /locus_tag="PK460_04040"
     CDS             complement(7177..7446)
                     /locus_tag="PK460_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54277.1"
                     /translation="MFKNVIARQKLTINSDYYAVIKLNNIDATKILIILNKGSVSGTL
                     SSAKEFDLIGTFKNNIKCPAFLVPNGTCTIKIKEEGKVNLKVLEH"
     gene            complement(7473..8033)
                     /locus_tag="PK460_04045"
     CDS             complement(7473..8033)
                     /locus_tag="PK460_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOE54278.1"
                     /translation="MKQSTNKVEFILDEYEYFEDLYIENGASDVRLVKTKRPNVRVVA
                     VNLSKYRVFVEKSILFVKEEHEYKYRAPFAAKKNNKRITIYLPENKYNDISIYSHYGA
                     KVVLPKTISFATIDASVAYGTLICSAPAKHLEVVHAESEIIIKDNKYETLYIDTTDSL
                     TKLQFIEHHLNLVKRFSLLMLAPQKI"
     gene            complement(8142..9107)
                     /locus_tag="PK460_04050"
     CDS             complement(8142..9107)
                     /locus_tag="PK460_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOE54279.1"
                     /translation="MNKLEKILTIVASSLLVVGLVIFISVLAVNGFNLGGLSTVKYEA
                     VTHEVSEDYAKIQFDISVGDVTIVKGEEATTKAICYENKRLTYNLNVEEDTLVVTDNN
                     TMRRFWTIFDLWGNKGITLYVPHDTYTSLLVKNNTGTLNVSSSVTFNTITARLETGSI
                     TLNGDASVVDLSSETGSITLNNVDADSARLVTATGRHVLNNVNVTGNLNIEVDTGTVK
                     LTNVKSDNLTVDSDTGTITLKDTVVTNTMRLAASTGSINFDKSDAHDIYARTSTGSIK
                     GSLLTGKVFSTKTNTGTISVPSNTAGAGVCDLRTDTGVIKITIAA"
     gene            complement(9104..9394)
                     /locus_tag="PK460_04055"
     CDS             complement(9104..9394)
                     /locus_tag="PK460_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54280.1"
                     /translation="MLIGAIAVFFSLVISLWAVDLGLFVGSFEAFITVFTLLFAGEGL
                     KALAYFGISLAMAGLGILLLLVGVKLTKYTIELAKKLINKIKTKMVLKGAKK"
     gene            complement(9578..9706)
                     /locus_tag="PK460_04060"
     CDS             complement(9578..9706)
                     /locus_tag="PK460_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54281.1"
                     /translation="MTKKEFLSALREKMAGLPKDDVDDRIAFYSEMIDDRMEEGLS"
     gene            complement(9699..10019)
                     /locus_tag="PK460_04065"
     CDS             complement(9699..10019)
                     /locus_tag="PK460_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOE54282.1"
                     /translation="MDVQLKRGLLEVCVLAVIRNKPTYGYRIIKDMKPYVEISESTLY
                     TILKRLEENEMLTVKTVEHNSRLRKYYSITGRGLGRIAEFKEYWKDMEAMYKFIIKKE
                     SSDD"
     gene            complement(10162..10668)
                     /locus_tag="PK460_04070"
     CDS             complement(10162..10668)
                     /locus_tag="PK460_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54283.1"
                     /translation="METKNINTKQLSIIVRIGIIFLFIIGLIMVIVWYPFTISLTIIG
                     PVDVEPTQLQKIQVYAQSAFYYLTSVPCFIILIFLWIIAGRLKKNGRFIAKDVRLLNA
                     ANIMLLIDLAIFLVGNIIIVFLEINFFVLAHFFIAVIGLVVLIFMWTTTLLIRRNVEL
                     QALIETDS"
BASE COUNT         3344 a         2236 c         1621 g         3488 t
ORIGIN      
        1 ttgtttttaa aaatttaatc tttatttgta aagataagta tgttatctaa ccaatatttt
       61 cacttattag gtaaaaatat cccgaattcc acttttttta atcaaaatta gttttattga
      121 atgagttcaa tccctaaaag taaggatgat aaagtaatac caaaggcttc actaatgcgg
      181 tagagaagcg ctaaagtggg gttacgtctt ccgttttcta aatcacataa ataattgtga
      241 ttcacattcg cccgtactga aagttctaaa agactccagc cctttgcttt tcgtaattgc
      301 cgtaatctaa tcccaaaggc ttcatttatt tccacgactt aactccttaa agggttctgc
      361 tagttcccgt aacttaataa ataaatgatt aacattactt ttagtaattc ttttttcact
      421 tcctaattct tctgttaaca tttccgcgag ttcattcatt gtcgcgtctt cattttctaa
      481 acgtaaacgc atcagtgctc cgagtttttc attattatgc ggagtatgtc cgtacactct
      541 ttgtacaatc ttaatgtttt caatttgttc agtggcactt ttaactgttt tattgtaatt
      601 agcttcgtcg catataaaca tccggttcgt aatattttgc atgtcgcggt ccattctgat
      661 cccttcaaat tctaaactag cgttcgccgc tccgacaaag acaagaaacg acgaaatttg
      721 gtcacttcgc tttaagtaca caatatattt atcacgtctt gcagttactt tagcgttaaa
      781 gtttgtcttt tcaaaacggt taagcattcg cgctaagtgt tcggcgtatt tcttttggcg
      841 cacaacaatt tctaaatggt aattggaact taagggactt gtcattgaac cactgcctaa
      901 aaaagcaccc gtaaagtatc cacctaaagt atcatcacta tatacaatct tccgactcac
      961 agcgctatca taaaaatcaa ccgcaagtgt ttcaataatt tcatcaacct tttgttcaac
     1021 aatcacatta aatttcatct ttttcccaaa tttacgtttt gtaacatacg aaaatgttgg
     1081 ttcaaaatta aagatatctt taaataattg ataaatatat ttcgctacct tagcgttttc
     1141 cgtgctaagt tttaaaactt ctttcttctc acgaattatt aaatgacctg catgcttaat
     1201 gagcgcggca agtgttgctt tccgtcttgg taaggaatag gatattgtat taaaagtaat
     1261 atcttctttt gctcttgtag taaatgacac atttttcata cttatattat aaaggaaaag
     1321 ggggaatttc ccccaaattt cacttattta ctaattagtt attttcgttt cacaaaacga
     1381 ttcgccgcga gagcagcaat cgcaccgtcg tttgtagcgg ttacaatttg ccgtaatact
     1441 ttttgattca cgtcaccaat actaaagagc ccagggacac tcgtcatcat ttcttcattc
     1501 actaacacaa accctttatc gtcacggaca tcaaattcac ttaagaaatc cgcggatgca
     1561 tcacttccca caaacgggaa cacggctttg acttcaataa ctttgtcggt gccatcaatt
     1621 acattcttaa ttgcaacact agtgacacct ttagtgtccc ctaagatttc aactggaata
     1681 tagtttaagt ggaaagtaac gtttggcatt gctttaagtt cattaacaag ggattcatct
     1741 gctctaaagc gttcattacg atgaacaata tgcacatgtt taacgatttt acttacaaag
     1801 agtgcttctt caattgcact attaccagcg ccaataatca taatcgcatc atttttatat
     1861 aagttcccgt cacaaatagc gcagtaactt acacctttac cggtgtaacg cgcttcacct
     1921 ggtattttta gttttcgctc ttgggtaccc gttgcgacaa tgacagcttt tgcgtaaagc
     1981 gtctcttcat cactatgaat tacaaaataa tcgtcttctt tggtaatttt atttacatca
     2041 gcgtaaccca cttcaatccc taagctgcgt agttgttcaa acattttaaa ggcaaggtca
     2101 ggtccgctga tggtttcaat acctggataa ttttcgacgt cctgcgtaat attcattttg
     2161 ccgcctggcg catatttatc aacaatctta aatgtcgcat ttgcgcgcga aagataaatt
     2221 gccgccgtca tccccgccgg acccgcacca ataattaaaa tcggaataat ttctttattc
     2281 atcactttgc actactccta agacatcact aatgtcatct aatacatatg ttgcacttac
     2341 atcaaggcga tgcggacggc gatgttgatg gataaacgca acatcgactc ctgcgtcttg
     2401 accggctaag atatcacgcc aattatcacc aacaaaaagt gttttatcag gtgcactttt
     2461 tagcgttttc ataatgtgca ctaagccttc accacttggt ttctttttaa aaccgtcatc
     2521 aacacatacg agcgttttaa agtaggaatg aacaccgtgt ctttttaaaa tttgttcagt
     2581 gcttgctcta gctctattag tgagtaaagc taactcaata ttattatttt ttaaagtttt
     2641 aattaattct ttcgctcctt taaaaagcgt aatatcttta acgctatctg gtaagtattc
     2701 attatattta gctattaact cttctaaggg gtaacctggg aataagtacg gcattgtttc
     2761 gtcaagcgtt ggcccgttta aaaagagata gtcatccata ttaatgtctt tccgcggtaa
     2821 ataagtttca ctcattttaa tcccgatttt aacgagtcct aaatcactat cggcaatcgt
     2881 gccgtcaaga tcaagcacca ctaaatcgta tttactcttc atttttttct acactttctg
     2941 aaggttcatt acttactact tcagctgttc ctttctttct tgttaaaagt ttgtggaaat
     3001 caaagatatg atttaaaaca atgagggcaa cacccacgac aaagaagata agggcattat
     3061 actctgacca cttaccacct gctcccatgc ggaacattgg gttacgtaac ggttccataa
     3121 taaaccgcac taaaccatac cacacaatat acataaatgc catatcaaat ggtttacgat
     3181 atttcttaag tccttcacca acaccgtaac gaatcacaaa gtaacccgtt aaattaagcg
     3241 ctgattcaat aaggaagaag ggttgacggt atttccctaa aataaacatt tggcgtttaa
     3301 taaattcagg gagaaaccac cattttgaaa tatcagtaat atccccacca tagacttctt
     3361 gattaaagaa attaccccag cggccaattg cttgagcaac taagatcgtc ggaataatga
     3421 tgtctgcggc aaaaccaata tcataagctt tacgtctttt cacaaagaac cacaccccag
     3481 caataatccc aaaaagcgct ccgcccataa tcgctagacc accatctcga atcgcaaaaa
     3541 tcgttaacgg attttccgca aaacctgatt caggattatt ccattcacca atcacatacc
     3601 atatgcgtgc gccgataatt cccgctggaa aagcaacata gaaaacattt tctaaaatac
     3661 cgtgtctacc gtatttttta taaaattcgt ggtcagtaat cgctaatact aaaagcgcac
     3721 cagttagaat gaatattgcg taaaaagcga taacgggact atcaccaaaa gggataccgc
     3781 gtggtaatgg gaactttata atttccgcaa gtgttaagcc ttcaagcaat acaaaaagga
     3841 aaacaatact taaaacaccg cttataatcg ttactatttt actttctttt atccactctc
     3901 tttgtaacac attaaattta taatagtgta aaacaaaagc agcaataaaa gtgagtaaca
     3961 ttaaagcaaa gagaaaacta cctaaaccaa ttaacaaaga atgtaacaca ctatttgata
     4021 gcaccatcag tggtaaaaac agcattaaca ctgacactgc accgcttaag acactaatgg
     4081 cgattaaacg gtgtttacct ttgaacaagg tattaatatc ctcttctttt tttacaaatt
     4141 cattaagact taaaccaagg accgtcactg ctccaataaa gatcaagcca aatattacaa
     4201 ctaccacgag tgttaaatcc atattacttt ttctcctttt ttaagtaggg cgctaaatat
     4261 tctccagtat acgacccttt aactttaact atttcttctg gggtcccact agcgacaact
     4321 cttccaccat aattcccacc gcctggacct aaatcaatta tataatcagc aaccttaata
     4381 atatcaagat tatgctcaat aacaataaca gtatccccat tagcgacaat agcttggagt
     4441 gaagtaataa gccgcccaac atcatctgca tgtaagccgg ttgacggttc atcaagtaaa
     4501 tatagtgttt taccagtcgg acgcttttgt aattcaaagg caagttttaa gcgttgtgct
     4561 tccccgccac taagttctgg tgcaggttgt ccaagcttaa tataacctaa accaatatcg
     4621 tataaagttt gtaaacggcg tacaatctgc ggcctatttg caaagaattc aagcgcttct
     4681 tcgactgtca tattaagtac atcaacaatg tttttgccgc gataatgaat cgaaagcgtg
     4741 tcagcgtgat atctttgtcc gtcacacatt tcacatttta catagacatc aggtaaaaag
     4801 ttcatagcaa tgcgtgtaac acccgcccct tcacagtgtt cacatctacc acctttagtg
     4861 ttaaacgaaa attgactctt cgtgtaacct cgcgctctag cgtccacagt ttgcgcaaaa
     4921 agttcacgaa tgtcatcaaa cactttcgta taagtcgcag ggttactgcg cggtgttcta
     4981 ccaattgggt cttgatcaat attaattact ttatcaatat gttctaagcc ttcaatttta
     5041 gagtaagcac ctggataagt ttcaactcta cgtaaatgac cgtccactgc cttaaaaagt
     5101 acttcttcca ctaaacttga tttgcctgaa ccgctcacac ccgtgacaag tgttaaggta
     5161 ttaagcggga tacttacatc aatatgcctt aagttattcg ccgctgcgcc atatattttt
     5221 aagtaattac cgttcccagg catccgtcta ggcggtagcg gaattacttt acggcccgag
     5281 agataatcac cagtaattga ttttttacaa ttaatcacat cttccacagt gcccgttacc
     5341 actacttcac caccatgaat ccccgctcct ggaccaatat caataatatg atcagcgctt
     5401 ctaattgttt cttcatcgtg ctcgacaaca attagtgtat tccctaaatc aaccatgtcg
     5461 cgtaatgctt ggattaattt attattatca cgctgatgaa gaccgatact tggttcatca
     5521 agcacatata aaacaccggt taatttacta ccaatctgcg tcgcaagtct tatgcgctgt
     5581 gcttcgccac ctgaaagcgt cgcagcgctc ctcgcaagtg ttaagtaatc taagccgacc
     5641 tttactaaga aagttaaacg gtgatcaatt tcatcaagta caagttgact aattgtccta
     5701 tctgtcacac ttagttttgg tcgcgtttca tttaagaatt gctttaattc accaatttgt
     5761 aacattgtta agtcgtgaat gttcttaccg ttaatataca ccgctagagc gtgttcgtta
     5821 agacgcttcc cgttacatgt cgaacaagta acatcacgca taaacttctc ataatattga
     5881 cgcatgaatt cacttgttgt ttcttcataa agccgttcaa tcttattttt aacgccttca
     5941 atctttccgc gtcgatacat tgtattgccg ctaccagtaa tgatcttaaa gttatgttca
     6001 tttggactac caaaaagcgc tatttcttta tgtttatcac ttaattcttt aaatggcaca
     6061 tgcattggga tgttatagag ctcacataac ttttcaaatt cttgccattc aatattttta
     6121 gtaccgacaa tatttttata atattcaatc gcccctttag caagacttac aattttatcc
     6181 ggcactaaaa tgttgatgtc aacttcacgc ttaagtccta acccgcgaca ttctggacaa
     6241 aaacccattg gggcattaaa gctgaatagt cgcggttcta aacgcggaac actaaaacca
     6301 cagatgtgac acgcgttatg cgtagaaaaa agtgtttctt cattattaaa aagaataata
     6361 acgtacccat gtccataatc tagcgcggta ttaattcctt caaagatgcg tgatttattt
     6421 tcggctttga gcacaacacg attaatgact aaatcaatgt tatgacgttt atttttatca
     6481 aggtttccta cttcttcaag tgcttttatt tcaccattaa ttcgaatccg cacaaaccct
     6541 tcttttgcaa tctttgctag tgtatcttta tgtgtacctt tttcagcttt gacaagtggc
     6601 gctaaaattt ggattcttgt tttctctgga tattcaaaga ttttatttaa gatttgttcg
     6661 ggtgaggaac tactaattgg aatatcgtgc tgcggacagt aaggcattcc aatccgtgca
     6721 tataaaagcc gtaagtagtc ataaatctcc gtaattgttc caactgttga acgcgggtta
     6781 ttactcgttg acttttgttc aatgctaatt gcgggactaa gcccttcaat tgaatcaacg
     6841 tcaggcttag cgagattccc taaaaattgc cgagcgtaag cacttaagct ttcaacgtaa
     6901 cgcctttgcc cttcgttata tattgtattt aacgctaggg ttgattttcc gctaccggaa
     6961 agaccagtga acaccactag tttatttttg gggatattca cactaatgtt ttttaagttg
     7021 ttatccctag cgcctttaat aataatgtag tcttttttat ccatagcttt cattttacct
     7081 tattcataag gtatttagac aaattatgca cggtaagtaa aaaaggttaa gacacaatgc
     7141 cgttaaccac tttaaaataa aaggcgaaaa tttatattaa tgttcaagaa cttttaagtt
     7201 tactttgcct tcttctttaa ttttaatcgt acatgttcca tttggtacta aaaatgcggg
     7261 gcatttaata ttatttttga aagtacctat taaatcaaat tctttagcac ttgataaggt
     7321 tccactaaca ctacctttat ttagtataat tagaatcttt gtagcatcaa tattatttaa
     7381 tttaataacc gcataataat cactattaat tgtaagcttt tgtctagcaa taacattttt
     7441 aaacattagt tcaagattat aaacatctat ttttatatct tttgtggcgc taacattagt
     7501 aaactgaacc ttttcactaa attcaaatgg tgctctataa attgtaattt cgttaaagaa
     7561 tcagttgtat caatataaag cgtttcatat ttattatctt taataattat ttcactttca
     7621 gcatggacaa cttcaagatg tttggcgggg gcactacaaa taagtgtccc gtaagcaaca
     7681 ctagcgtcta tcgttgcaaa acttatagtt ttaggaagta caactttcgc gccataatgt
     7741 gaatatattg aaatatcatt atatttattt tcaggtaagt aaatagtaat ccgcttatta
     7801 ttctttttag cggcaaaagg agctctatat ttatattcat gctcttcttt aacaaaaagg
     7861 attgatttct cgacaaaaac acgatatttt gataaattaa ccgctactac acgcacattt
     7921 ggtcgctttg tttttacaag ccgcacatca ctagcgccgt tttcaatata cagatcttca
     7981 aaatattcat attcatccaa aataaattct actttgttag tactttgttt cattttaaga
     8041 gactcctatt tttctaattt tatcacgctt aatgtaacaa tacttttaaa taagaaaaag
     8101 tgcgttactt ctaacgcact cactcggggg acaaaattta actatgccgc aatcgtaatc
     8161 ttaatcactc cagtgtcagt gcgaagatca caaactccgg caccagcggt attacttggc
     8221 acactaatgg taccagtatt tgtcttagtg ctaaagactt taccagttaa gagtgaacct
     8281 ttaatactac ctgttgaagt tctagcgtaa atgtcatggg catcactttt atcaaagtta
     8341 atggagcccg tactagcggc taaacgcatt gtatttgtca caactgtatc ttttaaagta
     8401 atcgtaccag tatcactatc aactgttaaa ttatcacttt taacattcgt taacttaact
     8461 gtgccagtat caacttcaat atttaggttt cctgttacat taacattatt taagacgtgt
     8521 cttccagttg ccgtcactaa tctagcgcta tctgcgtcaa cattatttaa agtgattgag
     8581 cctgtttccg agctaagatc tacgacactt gcatcaccat ttagtgtgat cgaccccgtt
     8641 tctaatcgtg ccgtaatcgt gttaaaagtt acgctgcttg aaacatttaa tgtccccgta
     8701 ttatttttca ctaaaagtga agtataagta tcatgcggaa cataaagcgt aataccttta
     8761 ttaccccata aatcaaagat agtccagaaa cgacgcatcg tattattgtc agtaacaaca
     8821 agcgtgtctt cttcaacgtt aaggttataa gttaagcgtt tattttcata acaaatcgct
     8881 ttagtcgttg cttcttcacc tttaacaatc gtgacatccc caacactgat atcaaattga
     8941 atctttgcat aatcttcact aacttcatga gttacagctt catatttcac cgtacttaat
     9001 cctcctaaat taaagccgtt taccgctaaa acactaataa atatgactag gccaactacg
     9061 agcaaggaac tcgcaactat cgttaaaatc ttttctaact tattcatttt tttgctccct
     9121 tcaacaccat ctttgtttta attttgttaa taagcttttt cgctaactca attgtgtact
     9181 tcgttaactt aacacccact agtaataaaa ggatccctag tcccgccatt gcaagactta
     9241 tcccgaaata ggctagtgct ttaagtcctt caccagcaaa aagcaaagta aagacagtaa
     9301 taaacgcttc aaatgagccg acaaaaagac caaggtctac ggcccagagc gaaattacaa
     9361 gcgagaaaaa caccgcaatt gccccgataa gcaaaccgcc taatccaata agaagcggta
     9421 cccaaagcgg tgcaagtaca ataattagaa caatttccca tccttctaac tttcgttttg
     9481 gtgttacttt gtctttaaca atcttagtaa ttgatgtgcc ttcaatttgt ttaatgacga
     9541 tctcatcaac attaccaact tcggcgaccg cttctgctta acttagtcct tcttccatgc
     9601 ggtcatcaat catttcgcta taaaaagcaa tacgatcgtc cacatcgtct tttggtaatc
     9661 ctgccatctt ttcacgcaag gcacttaaaa actccttttt agtcatcact actctccttc
     9721 ttaataataa atttgtacat cgcttccatg tctttccagt attctttaaa ttcagcaatg
     9781 cggccaagcc ctctccctgt aatactgtaa tatttccgga ggcgagaatt gtgttcaact
     9841 gtcttaacag ttaacatctc attttcttcg agacgcttta atattgtgta taatgtcgat
     9901 tcagatattt caacatatgg cttcatatct ttaataatac ggtagccata agttggttta
     9961 ttacgaataa cagcaagtac acaaacttct aagagtcctc gttttagttg tacatccaaa
    10021 gtcttatacc tcccttaaca taatacatcg taagccgaag tatataaaag tcaattcata
    10081 ttattaaaaa tttatatttt cttttaaatt gaaaaaagag tagttataaa ctactaaatt
    10141 taaaaatgat taaggacatt attaagaatc agtttcaatc agtgcttgta attcaacatt
    10201 acgcctaata agtaatgttg ttgtccacat aaaaattaat acgacaagcc caattacagc
    10261 aataaagaaa tgcgcaagta caaagaaatt aatttctaag aaaacaataa taatattacc
    10321 aactaaaaat atcgctaaat caattaataa cataatatta gcagcgttaa gcaaacgcac
    10381 atctttagcg ataaaccgac catttttctt taagcgacca gcgattatcc acaaaaatat
    10441 taaaatgata aaacatggca ctgatgtaag ataataaaac gctgattgcg catatacttg
    10501 aatcttttgt aactgtgttg gttcaacatc aactgggccg ataattgtaa gcgaaatagt
    10561 aaaaggatac cagacaataa ccataattaa gccgatgata aaaagaaaaa taataccaat
    10621 gcggacaatg atcgataatt gtttcgtatt tatgttttta gtttccataa cataattata
    10681 acgctaaaa
//
LOCUS       DAOSLP010000037        10583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_6146_length_10583_cov_12.637823, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000037 DAOSLP010000000
VERSION     DAOSLP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10583
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6146_length_10583_cov_12.637823"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1670
                     /locus_tag="PK460_04075"
     CDS             129..1670
                     /locus_tag="PK460_04075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOE54284.1"
                     /translation="MEKKEKLVLAVDFGTQSLRVSIVNQFGEIKAIIKNKYNEAYFSS
                     EPCFAEQNPNYYWYELCNATYKLKEKHDDLLQKVLAMSVTTFRDSPVFLDKNYEPTRP
                     MVLWLDQRQAALHKKPPFLRRLIFAIVGMSETVTLNMKRTPAIWVQENEKDVWEKTAY
                     YVNFSTYINYKLIGELIDSVANQTGHFPIHFKKGKWYGDNALKNIYRVPRTKLAKIVK
                     QGTVMGVISEAASKATGLPQGLKIIAAGTDKGSEAFGTGAYRKDIASISYGTASSISV
                     PNKKYIEPELFLPAYSAPIDGLYQMEVQIYRGYWMIGWFIKQFGTHETLEAKIEIMAT
                     EEVLNNKMLEIPPGSQGLVLQPYWGPGLKRPEARGAIVGFSDYHTRIHVYRAIIEGIA
                     FGLKEGLLGIEKRQHKKVKEIRVSGGGSQSDAICQITADIFGVPVSRTQTYETATIGT
                     ALVTFYYLGVFDSLETATKAMVHIEKTFIPNPKNVKQYNKLFKDVYAKLYPKLKGIYK
                     KLKYF"
     gene            complement(1728..3563)
                     /locus_tag="PK460_04080"
     CDS             complement(1728..3563)
                     /locus_tag="PK460_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE54285.1"
                     /translation="MARHGRGRKMEKARNSKLAWRGLLREFKPYRWRIIIIAVLLTIA
                     NVANALIPLLLRNILGAFSPENVRYGRYFLDVTNLALGLDWTKVFIDFGLMIGVIVAA
                     ALLGTIAQLIAIKISGAYAYNLRNKIRAKIDRLPLSYFDKHPHGELLSIGTNDVDAIA
                     QTLNNTLITVVSSITLFVAILIGMLLVNWRLALVALGMLPFVVIITLVITKNSQNQFI
                     TFQKKTGELEGVVEENFSGLQVIQLFNQQDKQLETFTNLNDSMAKSNFWSQWLSSFIF
                     PSIRFINNLGFVGVLLVAGVINDVISLAPFIFFLNNFMQPFLNLGQISANIQTTLAGA
                     ERVFNLLNAAEQEKEPEDALTTNDHVVGEFCFKDVDFSYNPDVELIKNMNLHVKPGDT
                     IAIVGPTGAGKTTLVNLIMRFYEINGGSITLDGTDIRNYSRSALRRSVGMVLQDTWLF
                     KGSIKNNIRYGNRDATDEEVIEAAKAARAHHFITTFPGEYEFILNEDGTNISQGQRQL
                     ITIARAIISKPKILILDEATSSVDTRTEYAIQSVMNEIMKDRTTFIIAHRLSTIKNAK
                     KILVMNKGNIIETGTHEELLAANGFYAGLYNAQFLGIDDSQETVD"
     gene            complement(3563..4795)
                     /locus_tag="PK460_04085"
     CDS             complement(3563..4795)
                     /locus_tag="PK460_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE54286.1"
                     /translation="MTLLLREGLTGVRVIRAFNQEERENRFFDEGNRKMTDIIIKVSN
                     VMQIISPIINILFNLVFFGIYLIGFVLFDKMLLGVIPFNMNAFSQTITAMMYAQHIMM
                     SFMMLSFTFIMFPRVKVSAKRINAVLNVKPNIVDPEAPLDKELIDTGIVKFENVTFTF
                     SDLEHPTLKNINFTAEPGTTTAIVGSTGSGKSSIINLLPRFFDASVGKVTFDGYDVRD
                     FKLNDLRDKIGFVPQTAVLFKGTIRDNLRFGNENATDEEMWEALRVAQAAGFVSKLEL
                     GLDAPVAQGGKNFSGGQRQRLAIARALVRKPKIYVFDDSFSALDFKTDIRLRSALKDY
                     AKDATIFVVAQRVNSILDAHQILVLDNGEVAGIGKHADLLNSCKVYQEIVTSQLNPEE
                     IKKSRDMFKDFTTEGGKA"
     gene            complement(4740..5396)
                     /locus_tag="PK460_04090"
     CDS             complement(4740..5396)
                     /locus_tag="PK460_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOE54287.1"
                     /translation="MFRLYKLVKRYWWQLAIIVVLVATQTLLQLQLPDYITTTQNIVN
                     NIGETGITRAEINEMLKQSGIMLLISLGIFALAAGQGSLNSKFVATLGKEIRAEVFKK
                     VENFSLSDVNEFGTASLLTRLINDSRAVQEFAFMVNRVFIMSPIFLIIGSVKLLTLDP
                     MYFYVVIVIIPLIIIVLIITFYYFCRSKPAFHPNSRTSGLCDAIITRRTNWCSCHSCL
                     "
     gene            complement(5397..6005)
                     /locus_tag="PK460_04095"
     CDS             complement(5397..6005)
                     /locus_tag="PK460_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE54288.1"
                     /translation="MARRTTSRTKSTTKTRRDDILEAGIRLFARVPFSELTIAAVARE
                     ANCGHSLVYHYFTNINRLYDECVNHVVARYKPFIEHFKSLTIPAELALAGSIALIVEM
                     LKREKLAPYCLSLLLYTYKQAPRNKAILTVREDWSKLFLNLISEGQKNERIISTLSAN
                     EILNVLKSILRGLISDQIVSGPSPANILTASEIYLPIMQGIN"
     gene            complement(6058..6753)
                     /locus_tag="PK460_04100"
     CDS             complement(6058..6753)
                     /locus_tag="PK460_04100"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE54289.1"
                     /translation="MRLDRFLAKTLGETRTTVKKYIRTKKVTVNGEVIISDGFQVDTI
                     NDVVAFDGEVLQYEEFQYYLINKPEGYVCANEDNVNPTIISFAPEFMMYKLHTVGRLD
                     KDTTGALLLTNNGKLTHQLISPKKNVEKVYLATTDLPIKGELVAHFARGIKIDEDFTT
                     LPATLEIVESNVGRVTVVEGKFHQIKRMFQAFGLNVVKLHREQFAGLRVDDLEIGEYR
                     KLEHDEINLLIKD"
     gene            complement(6750..7424)
                     /locus_tag="PK460_04105"
     CDS             complement(6750..7424)
                     /locus_tag="PK460_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOE54290.1"
                     /translation="MEWEITFLRWVVENTQTPFITAIFKFLTLSGELAVLYFIIAFIM
                     IGFKKTRKAGLIIVFGLILVSGFNLWIFKPIVNRPRPFDVPEFSADALWLNNYMYNVF
                     APQSKLNDFLVPGSKAFMSGHTLSSFIFGFTILFHHRKWSVPALILSVIMSYTRIYFG
                     FHYPTDVFAGIITALATVLLFTWLANKHETWFINLWRKFISLFKKKSKVDDTPAIETE
                     HQEEAQ"
     gene            complement(7449..8378)
                     /locus_tag="PK460_04110"
     CDS             complement(7449..8378)
                     /locus_tag="PK460_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOE54291.1"
                     /translation="MNRFLKRFLQGLLLGFALITAIGGGTMAVLIGVYDELINAVSDF
                     RKDPKKSLSLLGPMALGGIVSMGALFYPIKLALEHFPFLSISFFAGLTLGGLRIFTGT
                     IKGKVTWKNLLLTLAGFLFVFGLGTFTWFTTLHADLVNINFLQVILLFFVAFLSMGGH
                     VSAGISGTFILIAFGYYEELIDFLIRLAKFDFVNIGFDLAGLLSFGIGSILGLIVIAK
                     TYKYFFEKDRVKTYFTIFGFIIGSIVIAFFNGKVRPEYAKVTGFDPLMFTLCVVAVIV
                     GAALSYFLLGLANKKEKQQTVEEVAINSNNKED"
     gene            8425..9006
                     /gene="pth"
                     /locus_tag="PK460_04115"
     CDS             8425..9006
                     /gene="pth"
                     /locus_tag="PK460_04115"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOE54292.1"
                     /translation="MKLFVGLGNPGKKYENTRHNAGFLALDKFAEIAKVDIDKSDFKG
                     LFTSFTYNAEKIYLLKPQTYMNLSGQSVVSLMNFFKIEVDELYVIYDDMDISPGQIRL
                     KEDGSSGGHKGMQNIIELLGTTNIKRIRIGIGKPEHDGVDHVLQKLTDEDKKIFMEGV
                     TKASVAVRDLLNEDFNTVMNKNNRKEPKPPPSD"
     gene            8999..>10583
                     /locus_tag="PK460_04120"
     CDS             8999..>10583
                     /locus_tag="PK460_04120"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOE54293.1"
                     /translation="MIKLFETLFPKNTPPRFEKKRVFVTSEIIGTTLLVAEAFKKKRE
                     NYVVLTNNLYNAQKIISTLQTFVSQDDILFFPNDDLLFTEVLTATKELEAQRIYTLFS
                     SLEKDKKYIYVANQSALLKSTLSPTSFAKQFFELKVGNTVKMSALTTKLVELGYKNVT
                     NVEQTMQFAKRGDIIDIYTVNEDFPTRIEFFDDEIESIRDFNVSTQVSIKTRDKVTML
                     PASENLLFPNEQKEFLKCLQEFFNVQKKDMTSAEEEMFATNLNASISSYLEGDNNALN
                     KYLLIYSLFQNSLLSYIDNPKVVFANEPQLLATYEIISNHAEHYVRDQARRFNLLPKT
                     QLYQPYNTLDHYSKYVTREFSEDGNEGELRISPVADLRSRDDFNLVVQKYYDDNYLIY
                     FSVNNNTQLEQVKNLLKELQLDFDLDDKSSDKRILITYDYFPHGFVINSCKTAVFTSK
                     ELFNITRNEMRFNARFKAGTVIRSHEQLTPGDYVVHEYHGIGQFMEIESLKVFGVVSD
                     YIKIKYFGNEELSIPMEQIH"
BASE COUNT         3770 a         1855 c         1780 g         3178 t
ORIGIN      
        1 cgctaattat aatatcatag atattaaggt ttattacaat aaatcactca aaaacagcga
       61 aaattgaaga gaaaacacta taatagtaat aatttgctaa aatagtaatg ctaaaggaaa
      121 aatcgaatat ggaaaagaaa gaaaaacttg tactagcagt cgactttggt acccaatctc
      181 taagagtctc gattgtaaac caatttggag aaatcaaagc gatcattaaa aataaatata
      241 acgaggctta ttttagtagt gagccttgtt ttgctgagca aaatccaaac tactattggt
      301 atgaattatg taacgccaca tataaattaa aagaaaagca tgatgacctt ttacagaaag
      361 tactagcgat gagtgtcacg actttccgtg atagcccagt atttcttgat aaaaactatg
      421 aaccaactag accaatggtg ttgtggcttg atcaacgtca agcagcgctc cataagaaac
      481 caccattttt aagacgctta attttcgcta ttgttgggat gagtgaaacg gtcactttaa
      541 atatgaaacg cacaccagcg atatgggtgc aagaaaatga aaaagatgtt tgggaaaaaa
      601 cagcgtatta tgttaatttc tctacttaca ttaactataa attaattggt gaacttattg
      661 atagcgtcgc taatcaaaca ggtcacttcc caatccattt taaaaaaggg aaatggtatg
      721 gtgataatgc attaaagaat atttatcgtg ttccgcgcac taaattagcg aaaattgtaa
      781 aacaaggaac ggtaatgggt gttataagcg aagccgcaag taaagcaaca ggacttccgc
      841 aaggattaaa aatcattgca gcgggaactg ataaaggaag cgaagcattt ggaacaggtg
      901 cgtaccgcaa agatattgct tcaatttcct atggcactgc aagttcaata tctgttccga
      961 ataaaaaata cattgaaccc gaattatttt taccagccta tagcgcacca attgatggtc
     1021 tttatcaaat ggaagtgcaa atatatcgtg gctactggat gattggctgg tttattaaac
     1081 aatttggtac gcatgaaaca cttgaagcca aaattgaaat aatggccact gaagaagtgt
     1141 taaataataa aatgcttgaa ataccaccag gctcacaagg actcgtctta caaccatatt
     1201 ggggtccagg acttaaacgc ccggaagctc gtggtgcgat tgttggattt agtgattatc
     1261 acacacgcat tcatgtttat cgggcgatta ttgaagggat tgcttttggt ttaaaagaag
     1321 ggttactcgg tattgaaaaa agacaacata aaaaagtaaa agaaataaga gtatcaggag
     1381 gcggttcgca aagtgatgcg atatgtcaaa ttaccgctga tatatttggt gtcccagtta
     1441 gtcggacaca aacttatgaa accgcaacaa ttggtacagc gcttgttact ttctattatt
     1501 taggtgtgtt tgattcactt gaaacagcca caaaagcgat ggtccacatt gaaaaaacat
     1561 ttataccaaa tcctaaaaat gttaaacagt ataataaatt atttaaagat gtatacgcta
     1621 aactttatcc aaaattaaaa ggcatatata agaaattaaa atacttctag aagaaaaaat
     1681 aaaagggtta atcgacaaat taaccctttt ttgctgtaaa taattgatta atccacagtt
     1741 tcttggctgt catcaatacc aaggaattgt gcattataaa gaccagcgta aaatccgttt
     1801 gctgctaaaa gttcctcgtg agttcccgtt tcaataatgt tacctttatt cattaccaaa
     1861 atcttcttag catttttaat ggtgcttaag cggtgggcaa taataaatgt agtgcggtct
     1921 ttcataattt cattcataac actttgaatc gcgtattccg tacgcgtatc aacactacta
     1981 gtagcttcat caagaataag gattttaggt ttagaaataa ttgcacgagc aatcgtaatt
     2041 aactggcgtt gcccttgcga aatattagtg ccatcttcat taagtataaa ctcgtattca
     2101 cccggaaaag tagttataaa gtggtgagcg cgcgctgctt tagcagcttc gataacttct
     2161 tcgtcagtag cgtcacgatt accataacga atattatttt taatcgaacc tttgaagagc
     2221 caagtatctt gtaaaaccat cccaacacta cggcgtaatg cacttcgtga ataattacga
     2281 atatcagtac catctaatgt aattgaacca ccatttattt catagaatcg cataattaaa
     2341 ttaactagtg ttgttttgcc cgcgccagtt ggaccaacaa tagcgattgt atcccctggt
     2401 ttaacatgta aattcatatt tttaataagt tcaacatcag ggttataact aaaatcaaca
     2461 tctttgaagc agaattcccc cacaacatga tcgtttgtag ttaatgcatc ctctggttct
     2521 ttttcttgtt cagcggcatt aagtaaatta aacactcttt ctgcaccagc aagtgttgtt
     2581 tgaatatttg cagatatttg ccctaagtta aggaatggtt gcataaagtt atttaagaaa
     2641 aaaataaatg gcgcgagact aataacatca tttataacac ccgctacaag taaaacacca
     2701 acgaagccaa gattattaat aaagcgaatt gatgggaaaa taaatgaact cagccattgt
     2761 gaccagaaat tagatttagc catagaatca ttaagatttg taaaagtttc tagttgttta
     2821 tcttgttgat taaatagttg aatgacttgg agtccggaaa agttttcttc cactacccct
     2881 tctaattcac ctgttttctt ttggaaagta ataaactgat tttgtgaatt tttcgtaata
     2941 acaagcgtaa taattactac aaacggaagc atgccaagtg ctactaaggc aagccgccaa
     3001 tttacaagta acatgccaat taaaattgca acaaaaagag tgattgatga aacaacagtg
     3061 ataagcgtgt tatttaatgt ttgggcaata gcatcaacat catttgtgcc aattgaaagt
     3121 aattcaccat gcggatgttt atcaaaataa gaaagtggta aacgatcaat tttcgcgcgt
     3181 attttattac gaagattata agcgtacgct ccagaaattt tgatagcaat cagttgtgcg
     3241 atagtaccaa gtaatgcagc ggcaacaata acaccaatca ttaatccaaa atcaatgaag
     3301 acctttgtcc aatctaaccc taacgctaga ttagtaacat ctaaaaaata acgaccataa
     3361 cgaacatttt caggcgagaa agcacccaga atattacgca ataatagcgg gataagcgca
     3421 ttagctacat ttgcgatagt aagaagcaca gcaataatta ttatccgcca acgataaggt
     3481 ttaaattcgc gcaaaagacc acgccaagct aattttgaat tgcgcgcttt ttccattttg
     3541 cgtccgcgtc cgtggcgtgc cattatgctt tacccccttc agttgtaaag tctttaaaca
     3601 tatcacgcga ctttttaatt tcttcaggat ttagttgtga tgtaacgatt tcttgatata
     3661 ccttacacga attaagtaag tcagcatgtt tgccaatacc agcaacctcg ccattatcaa
     3721 ggactaagat ttgatgggca tcaagaatag aattaacacg ctgtgctaca acaaaaatag
     3781 ttgcgtcttt tgcgtaatct tttaatgcgc ttcttagtct aatatcagtc ttaaagtcta
     3841 gtgcactaaa agaatcatca aacacataaa ttttaggttt tctaacaagt gcacgcgcta
     3901 tcgctaggcg ttgtctttga ccaccactga aatttttgcc accttgtgca actggtgcat
     3961 caagtccaag ttcgagttta gaaacaaaac ccgcagcttg cgccacgcgt aaagcttccc
     4021 acatttcttc atcagtagcg ttttcattac cgaagcgtaa attatcacga attgtacctt
     4081 taaataacac tgcagtttgt ggaacgaagc ctattttgtc gcgtaaatca tttaatttaa
     4141 agtcacgcac atcatagccg tcaaaagtta ctttaccaac actagcatca aaaaatcgtg
     4201 gtaataaatt aatgattgaa ctcttgccag aaccagtaga accaacaatt gctgttgtag
     4261 ttcctggctc tgctgtaaaa ttaatattct ttaatgttgg gtgttcaaga tcgctgaaag
     4321 taaaagttac attttcaaac ttaacaatac cagtatcaat taattcttta tcaagcggtg
     4381 cctccggatc aacaatattt ggtttaacat ttagtacagc gttaattcgt tttgcgctaa
     4441 ctttaacgcg cgggaacata ataaaggtaa agctaagcat catgaaactc atcataatat
     4501 gttgagcgta catcatcgct gttattgttt gactaaaagc attcatatta aaaggaataa
     4561 caccaaggag cattttatcg aacaagacaa aaccaataag atatattcca aaaaacacta
     4621 agttaaataa gatattaata atcggactta taatttgcat aacattacta actttaataa
     4681 taatgtcagt cattttacga ttgccttcgt caaagaagcg gttttcacgt tcttcttgat
     4741 taaaggcacg aatgacacga acaccagtta gtccttcgcg taataatagc gtcacataat
     4801 ccactcgttc ttgaatttgg gtgaaaagcg ggcttgctac ggcaaaagta ataaaaagta
     4861 ataatcaata caattataat caaaggaata ataacgataa cgacatagaa atacattgga
     4921 tctaatgtta gtaatttaac agaaccaata attaaaaaga ttggtgacat aataaaaaca
     4981 cgatttacca taaaagcaaa ttcttgcaca gcgcgtgaat cgttaattag cctagttaaa
     5041 agactagcgg ttccaaattc attaacatca cttaatgaaa agttttcaac ttttttgaac
     5101 acttccgcgc gaatttcttt accaagggta gcaacaaatt tagagtttaa tgagccttgg
     5161 cctgctgcta aagcaaaaat accaagggaa attaaaagca taataccgct ttgttttaac
     5221 atttcattta tttcagcgcg agttatccca gtttcaccaa tattattaac aatgttttga
     5281 gttgttgtaa tataatcagg taattgaagt tgaagcaatg tctgagtagc gacgaggaca
     5341 acaataatcg ctaattgcca ccaataacgt ttaactaatt tataaagtct aaacatttag
     5401 tttatcccct gcataattgg taagtaaatt tcagacgctg ttaagatatt agcgggcgac
     5461 ggaccagaaa caatttggtc actaattaaa ccgcgtaata ttgattttaa aacatttaat
     5521 atctcattag cgcttaatgt actgattata cgctcgtttt tttggccttc actaatgaga
     5581 tttaaaaaaa gttttgacca atcttcccgc actgttaata ttgctttatt ccgtggtgct
     5641 tgtttgtaag tatatagcaa cagacttaaa caatagggag ctagtttctc cctttttaac
     5701 atttctacaa tcaaagcaat tgaacccgcg agcgctagtt cagcgggaat tgttaatgat
     5761 ttaaagtgtt caataaaagg cttataacga gcaacaacat gattgacaca ttcatcatat
     5821 aagcgattaa tatttgtgaa ataatgataa acaagactat gaccacaatt agcttcgcgc
     5881 gcaacagcgg caattgttaa ttcagaaaaa gggacacgag caaaaagtcg tattcctgct
     5941 tcgagaatat cgtcgcgtcg agtttttgtg gtacttttag ttctacttgt tgttcgtctt
     6001 gccatagtta taccattgca aaaattataa caaagatgaa cctaaataga tattgaatta
     6061 atctttgatt aataaattaa tttcgtcatg ttctaatttt cgatattcac caatttctaa
     6121 atcatcaaca cgcagtcctg caaattgttc gcgatgaagt tttacaacat ttaacccaaa
     6181 cgcttgaaac atccgtttta tttgatgaaa tttaccttcc acaacagtaa ctctacctac
     6241 attagattcc actatttcta aagttgcagg aagtgtcgta aaatcttcat caattttaat
     6301 tccacgcgca aaatgcgcga ctaattcacc tttaataggt aaatcggtgg tagcaagata
     6361 aaccttttca acattctttt ttggacttat aagttggtgt gtaagtttac cgttgtttgt
     6421 taatagtaag gcgcctgtcg tatccttatc taaacgacca acagtatgta atttatacat
     6481 cataaattct ggcgcaaaag aaataattgt agggttaaca ttatcttcgt ttgcacacac
     6541 atatccttct ggcttattaa ttaaataata ttgaaattct tcatattgta aaacttcacc
     6601 atcaaaagcg acgacatcat taatagtgtc cacttgaaaa ccgtctgaaa taataacttc
     6661 accattgaca gtcacttttt tagtgcgaat gtatttttta acagtggttc tagtttcacc
     6721 gagagttttt gctagaaaac gatctaatct cattgtgctt cttcttgatg ctcagtttca
     6781 attgcgggag tatcgtctac tttagacttt ttcttgaaga gactaataaa tttacgccaa
     6841 agattgataa accaagtttc gtgtttattt gctaaccaag tgaaaagaag gacagtggca
     6901 agagctgtta tgatacccgc aaagacatca gtaggataat ggaatccaaa ataaatccgg
     6961 gtgtatgaca ttattacact taaaattaaa gcgggcactg accatttccg atgatggaat
     7021 aaaatagtaa atccaaaaat aaaagatgaa agcgtatgtc cagacataaa tgcttttgaa
     7081 ccaggcacta aaaagtcatt aagcttactt tgtggcgcaa agacgttata catatagttg
     7141 tttaaccata aagcatccgc agaaaattca ggaacatcaa aaggtcgcgg ccgattaaca
     7201 atcggtttaa aaatccaaag attaaaacca gatacaagaa ttaaaccaaa aacaatgatt
     7261 aatcctgctt ttctcgtctt tttaaatcca atcataataa aagcaattat aaaatagaga
     7321 actgcaagtt cgccagagag tgttaaaaac ttaaaaatag cggtaataaa cggtgtttgt
     7381 gtattttcta caacccagcg taaaaaagta atttcccatt ccatagttaa gtactccttt
     7441 tttatattct aatcttcttt attattacta tttatagcaa cttcttcaac tgtttgttgt
     7501 ttttcttttt tatttgctaa acctaaaagg aagtaagaaa gcgccgcgcc tacaataaca
     7561 gcaacaacac aaagagtaaa cattaagggg tcaaaaccag taactttagc gtactctggc
     7621 cgtaccttac cgttaaagaa agcaataaca attgagccaa taataaaacc aaagatcgtg
     7681 aaataagttt taacgcggtc tttttcaaag aaatatttat atgtcttagc aataacaatt
     7741 aaccctaaaa ttgaaccgat accaaacgat aagagtcccg ctaagtcaaa accgatattc
     7801 acaaaatcaa atttagcaag tctaattaag aaatcaatta attcttcata atatccaaaa
     7861 gcaatgagaa taaatgtacc tgatatacct gcagaaacat gaccgcccat tgaaagaaag
     7921 gccacaaaga aaagcaagat gacttgtaag aaattaatat ttactaaatc agcgtgtaaa
     7981 gtcgtaaacc aagtaaaggt tcctaagcca aatacaaaaa ggaaacccgc aagtgttaaa
     8041 agtaagtttt tccaagtgac tttgccttta atagtaccag taaatattcg taaaccgccg
     8101 agtgttaaac cagcaaaaaa tgaaattgaa agaaaaggaa agtgttcaag cgctagtttg
     8161 attggataaa aaagcgcgcc catacttaca ataccgccaa gcgccattgg tccgagtaaa
     8221 ctaagcgact tttttggatc tttcctaaaa tcagaaacag cattaattaa ctcatcataa
     8281 accccaatta aaacagccat agtcccgccg ccaatcgcgg taattaaggc aaatcctaag
     8341 agcaaacctt gtaaaaaacg ttttaagaat ctattcatat cataaataat agcacaataa
     8401 atgttataat ttatcagtaa aaatatgaaa ctatttgtcg gtttaggtaa tccaggaaag
     8461 aaatatgaaa acacgcgcca caacgctggg tttttagcgc ttgataaatt tgccgaaatc
     8521 gctaaggttg atattgataa aagtgatttc aaagggcttt tcacgagttt tacttacaac
     8581 gctgaaaaaa tatatttatt aaaaccacaa acatatatga atctttctgg acaatcagta
     8641 gtgtcactaa tgaacttctt taaaattgaa gttgatgaac tttatgtaat ttatgatgat
     8701 atggatattt ctccaggaca aatccgttta aaggaagacg ggtcatctgg cggacacaaa
     8761 ggaatgcaaa atattattga actcttagga accacaaata ttaaacggat tcgtattggc
     8821 attggtaaac cagaacacga cggagtggat catgttttac aaaaacttac tgatgaagat
     8881 aaaaagattt ttatggaagg cgtcactaaa gcaagcgttg ccgttcgtga tttgttaaat
     8941 gaagatttca atactgtaat gaataaaaac aaccgaaagg agccaaaacc cccaccgagt
     9001 gattaaactt tttgaaacat tgtttcctaa aaatacaccc cctcgctttg aaaaaaagcg
     9061 agtttttgtc acaagcgaaa tcatagggac aacgctatta gtcgccgagg cttttaagaa
     9121 aaagcgagaa aactatgttg ttttaacgaa caatttatat aatgcacaaa agataatttc
     9181 aaccttacaa acatttgtta gtcaagacga tattttattt ttcccaaatg acgatttatt
     9241 atttacggaa gtattgaccg caactaaaga actagaagca caaagaattt acacattatt
     9301 ttcatcactt gaaaaagata aaaaatatat ttatgttgct aaccaatcag cgctgcttaa
     9361 atcaacgctt tctccaacat cttttgccaa acaatttttc gaactaaaag taggaaatac
     9421 tgttaaaatg tcagcgttaa ccaccaaatt ggtggaatta ggatataaaa atgtcactaa
     9481 tgtcgaacag acgatgcagt ttgcaaaacg cggtgatatt atcgatatat atacagtaaa
     9541 cgaagacttt ccgacgcgta ttgaattctt cgatgatgag attgaatcaa tccgtgactt
     9601 taatgtaagc acacaagtat caattaaaac tcgtgataaa gtaactatgc ttcccgcaag
     9661 tgaaaactta ttattcccca atgaacaaaa agaatttctt aagtgcttgc aagaattttt
     9721 caatgtacag aaaaaagata tgactagcgc ggaagaagaa atgtttgcta ctaacttgaa
     9781 tgcaagtatt agtagttatt tagaaggtga taataacgct ttaaataaat atctattaat
     9841 ttattcattg tttcaaaata gtttgctctc ttatattgat aatccaaaag ttgtattcgc
     9901 taatgaacca caattacttg ctacatatga gattatttct aatcacgcag agcattatgt
     9961 tagagaccaa gcaagacgat ttaacttgtt accaaagaca caactttatc aaccttataa
    10021 cactttagac cactattcaa aatatgtaac acgagaattt agcgaggacg gcaatgaagg
    10081 tgaactccgt ataagtccgg tagcggatct tcgaagtcgt gatgatttta atttagtagt
    10141 gcaaaaatat tatgatgata attatcttat ttatttctca gttaacaata acacacaact
    10201 agagcaagtt aaaaatttat taaaagaatt acaattagat tttgatttag atgataaaag
    10261 tagcgataaa agaatattaa ttacctatga ctattttccg catggatttg tcattaattc
    10321 ctgcaaaaca gctgtattta ctagtaaaga gctatttaat attacgcgta atgagatgcg
    10381 gtttaatgcc aggtttaaag ccggaactgt gattcgtagt catgaacaac ttaccccggg
    10441 tgattatgta gttcatgaat atcacggtat tggtcaattt atggaaattg agtccttaaa
    10501 agtatttggt gttgttagtg actatattaa aattaaatat tttggcaatg aagaattatc
    10561 aattccaatg gaacaaattc att
//
LOCUS       DAOSLP010000038         8810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_7497_length_8810_cov_9.844889, whole genome shotgun sequence.
ACCESSION   DAOSLP010000038 DAOSLP010000000
VERSION     DAOSLP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7497_length_8810_cov_9.844889"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1711
                     /locus_tag="PK460_04125"
     CDS             206..1711
                     /locus_tag="PK460_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE54294.1"
                     /translation="MKRKYVFLPLLLLGSVSLSSSPSPRVTHFAPHYAAPSTEPSGPQ
                     TLRIYNWEEYISEEDIDDPDCIDVLAAFQEYYLENYGEEVTIEYNTFSTNEDMYNIVK
                     LNGAKYDLIAPSDYMIQRMIREDMLEKYDLDAEGNYKYVPNYNEYASPYLIDLFKEDL
                     GIYEYAPGYMWGTMGFLYNSEFNDTINEDVQSWMMLWDKKYSKKTSIKDSMREGYLMG
                     LFKYYEDELNDLAAQREAGLISDDYYNHELSDLLNDVDPDTIKEVIHVMNELKNNIYG
                     LEVDQGKIDMITGKIAANTAWSGDAVYAIEEDPNDVLRYAIPKEGSNIWFDGWIMPKG
                     ANRVVAQRFVNFVSSPEMAIANMYYIGYTSFIAGDEVLEYVLDNDAVYEAAEAEETVE
                     LDFTYFFSDTIENPSDALITIDASYVGHTLTATYPTEEEIVRSVVMRDFGDSNKLVID
                     MWANFKATDIQNWMIIFSVIIVLAVGSAVTLNQVQKHKAARRKRNLYKDKQ"
     gene            1708..2829
                     /locus_tag="PK460_04130"
     CDS             1708..2829
                     /locus_tag="PK460_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE54295.1"
                     /translation="MKKRLAVIGGGVSGVTLARLVQGSFNVTIFERESSLLQKLLRTG
                     NGRANIYNRNIHSFAYNDEHFLSEHLPHIVKTLDDLFSAFNIVTYTDTEGRVYPYSRS
                     AKALRALLIKDLKAEVKLEHNVEEIVKVRDKYKVDGEEFDAVALTMGSSAGLFKYPLD
                     NGNAALLSALDLETTALVPTIKTIKVKENLKRIEHERITARVTLMDDENVIASDVGEV
                     LFKKDGLSGIVSFILSSYLEWAVVRYPKRNYYLTLDLMPEFSARYTAEIIKTTDDLDN
                     VFSPNISHYLKGRNEPLLDRMKALTFTPIPKNNPENTSAMHGGIKLSKLNTDFEVRES
                     PNLFALGEVINIDGICGGYNLAFAFYSAKVVSDALLRKL"
     gene            3095..5299
                     /locus_tag="PK460_04135"
     CDS             3095..5299
                     /locus_tag="PK460_04135"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOE54296.1"
                     /translation="MVTHIKKRDGRLQKFNAGKIANAINAAYGNTNDVQSLLYAAEIT
                     SNLNTNYDGTIDIEVVQDEVEKFLMKKDPNIAKKYILYRKDRERIRNAKSTVFNYKKL
                     VEDYLNIADWRVKENSTVNYSLGGLILSNSGAVTANYWLSEVYDEEVANAHRSAAIHI
                     HDLSMLSAYCAGWDIEKIIRDGVGGVEGKISSKPAKHLFTLANQLVNFLGIMQNEWAG
                     AQAFSSFDTYLAPFVKADNLTYAEVKQAIQSFVFGVNTPSRWGSQAPFSNITLDWTVP
                     SDLADRPAIVGGKDMPFKYKDCQKEMDMINKAFLEVMIEGDANGRGFQYPIPTYSITR
                     DFSWEETENNKLLFEMTAKYGTPYFSNYINSDMEPSDVRSMCCRLRLDLRELRKKGGG
                     FFGAGSNTGSVGVVTLNMPRLAYLSRTEVEFFNELDKLMDISARSLKVKRDVVSKLLE
                     DGLFPYTKRYLGNFDNHFSTIGLVGMNEACLNARWLGKDLGDPESQAFTIKVLNHMRE
                     KLSDYQEAYGDLYNLEATPAESTAYRFAKHDKVRYPDIITARLGEDGDPFYTNSSHLP
                     VGYTASISEALDIQDEMQTLYTSGTVFHTFLGQKLPNWQTAARLVRKIAENYRLPYFT
                     MSPTYSICPTHGYLTGEVEICPECKRTTEVYSRITGYYRPLKNWNDGKKAEFENRLEY
                     ADFTIEGGTVHNPKFNAPKVEVKDTADAPIKEQKPTPHKGAPIIFLNKQNND"
     gene            5292..5810
                     /gene="nrdG"
                     /locus_tag="PK460_04140"
     CDS             5292..5810
                     /gene="nrdG"
                     /locus_tag="PK460_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019000008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOE54297.1"
                     /translation="MTKKKIRIAGYVNDSLVDGPGIRFTIFTQGCPYHCLGCHNPDTW
                     DPKGGEEVEISTLIAKWRKNSLLDGITLSGGEPLLQKEALLPLINAARKDGLNITMYT
                     GSTYEELIHRNDPLVNEILSKVDYLIDGPFILHELNLNLLYRGSNNQRIIDLRKTTPN
                     NIVLINTMHDFK"
     gene            complement(5877..7025)
                     /locus_tag="PK460_04145"
     CDS             complement(5877..7025)
                     /locus_tag="PK460_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE54298.1"
                     /translation="MKNLWTVIKKELKRFFTDKRMLAALILPGVIVYILYSIMGNVMQ
                     STMEGKDHEYVIRVENRPNDPRFLAIETLIADPEVGFKKVTFLDETPEDFEAMIAEKK
                     LDLYVVYNTDFLAKYNANDPNDPPVVTFHYDESVIPSLNAYSTYAGLLNASLQTYVAV
                     PINHSAEGGIDTTIIKGLLPFLLITFIFTGAMAVAPESIAGEKERGTIATLLVTPVKR
                     STLALGKVTALSIVALVSAASSFLGVMLSLPKMMGSELSLATFTAGTYLAIFAVLIVS
                     MLVFIVLNSIISAYAKSIKEASGLAAPLMMVIMAVGVTTMFGSAATNNALYLIPIYNS
                     VNILLSLFGGEFLVVPFILTMLSNLLLTAGGVYLLTVMFSNEKIMFRK"
     gene            complement(7025..7801)
                     /locus_tag="PK460_04150"
     CDS             complement(7025..7801)
                     /locus_tag="PK460_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE54299.1"
                     /translation="MQPILEVKNLVKTFKLSKKQQKIEKTNTKVRVAVNDLSFNVYEG
                     EIYGLLGPNGAGKTTTLRIISTLIKKDNGEVKVNGYDISKEQSKVRSQIGFLTSELKL
                     DDFFTPNYLFDYFSHLHGVPEDVIEKRKQELFDKFGISEFKEVKVSDLSSGMKQKVSL
                     VISVVHDPKFIIFDEPTNGLDVITAKTVIDFLVALKEAGKTVILSTHIFSLVERICDR
                     VGVIIDGALIFEEPMSTFSEPNSLEERFFLEYAKVKGDLA"
     gene            8022..8294
                     /locus_tag="PK460_04155"
     CDS             8022..8294
                     /locus_tag="PK460_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOE54300.1"
                     /translation="MSMTNLNIRMDKKVKEEAEAIFQELGLNMTTAVNIFLRTTIREK
                     GIPFHLSLSVSNVTTKAAIEEGRKIALTDNVKGYDTVSDLKAALED"
     gene            8394..8467
                     /locus_tag="PK460_04160"
     tRNA            8394..8467
                     /locus_tag="PK460_04160"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8426..8428,aa:Cys,seq:gca)
BASE COUNT         2905 a         1519 c         1749 g         2637 t
ORIGIN      
        1 ctatcttcgt ataaacgcga atgatgaaac gtctaagcgt tacttaactt attaacgatg
       61 gtgatagaaa gccgtgccct gaaaaacctc caagccatca agtggcaaac aaatgagaca
      121 ggtgcacagc tattacattg tagcctagaa aggagagaag ttattcagtc atcaaactag
      181 gcttaactga ataatacgta ctataatgaa acgtaaatat gttttcttac cactattatt
      241 acttggtagt gtaagtttaa gtagttcacc ttcgccaaga gtaacgcatt tcgcgccgca
      301 ctatgccgct ccaagcactg agccaagcgg tccgcaaact ttacgcattt ataactggga
      361 agaatatatt agtgaagaag atattgatga tccagattgt attgatgttt tagcggcctt
      421 tcaagaatat tatttagaaa attatggtga agaagtcaca attgagtaca atacattttc
      481 aacgaatgaa gatatgtata atatcgtcaa actcaatggc gcaaagtatg acttaattgc
      541 cccaagtgac tatatgattc aacggatgat tcgtgaagat atgctcgaaa agtatgatct
      601 tgacgcagaa ggtaattata aatatgttcc taattataat gaatatgcaa gtccatattt
      661 aattgatctt tttaaagaag atttaggaat ttatgaatac gccccagggt atatgtgggg
      721 gacaatgggt tttctttata acagtgaatt taatgacacc attaatgaag atgtccaaag
      781 ttggatgatg ttatgggata aaaaatatag taagaaaact tcgatcaaag atagtatgcg
      841 cgaaggttat ttaatgggac tttttaaata ttacgaagat gaacttaatg atttagccgc
      901 gcagagggaa gcagggctta ttagtgatga ttattacaat cacgagttaa gcgacctact
      961 taatgatgtt gaccctgata cgattaaaga agtgattcac gtgatgaatg aacttaaaaa
     1021 taatatttat gggttagaag ttgaccaagg taagattgat atgattactg ggaaaatcgc
     1081 tgcgaacacc gcgtggagcg gggacgcagt gtatgccatt gaagaagacc caaatgatgt
     1141 gctccgttac gctattccaa aagaaggtag taatatttgg tttgatggct ggattatgcc
     1201 caagggcgct aatcgtgtcg tcgcacaacg ctttgtgaac tttgtgagta gtccggaaat
     1261 ggcaatcgct aatatgtatt acattggtta cactagtttt attgcggggg atgaagtact
     1321 tgaatatgtg cttgataacg atgcagtgta tgaggcagca gaagcagaag aaacagttga
     1381 gcttgacttt acatatttct ttagtgatac aatcgaaaat cctagtgacg ccttaattac
     1441 gattgatgcg agttacgttg gtcatacatt aaccgcgact tatccgacgg aagaagaaat
     1501 cgtccgcagt gtcgtcatgc gtgactttgg tgatagtaat aagctcgtca ttgatatgtg
     1561 ggcaaacttt aaagcgacgg acattcaaaa ctggatgatt atctttagtg ttatcatcgt
     1621 tttagcagtt ggtagtgccg tcacccttaa ccaagtccaa aaacataagg cagcgcggcg
     1681 taagcgcaat ttatataaag ataaacaatg aaaaaaagat tagcagtcat cggtggtgga
     1741 gtaagcggag ttaccttagc gcgacttgtg caaggttcct ttaatgtcac catctttgag
     1801 cgtgaatcta gtctcttaca aaaactttta cgcacgggta atggacgcgc gaacatttat
     1861 aatcgcaaca tacattcctt tgcttataat gatgaacact ttttaagtga acacttacca
     1921 catatcgtta aaacgcttga tgacttattt agtgctttta atattgtcac ttacacggat
     1981 acagaaggaa gagtatatcc ctatagcaga agtgctaaag cactccgcgc attactcatt
     2041 aaagatttaa aggcagaagt taagttagaa cataatgtcg aagaaatcgt taaagttcgt
     2101 gataaatata aagttgatgg tgaagaattt gacgctgttg cgttaacaat gggcagtagc
     2161 gcgggacttt ttaagtatcc gcttgataac ggtaacgcgg cgcttttaag tgctttagat
     2221 ttagaaacaa ctgcgctagt accaacgatt aaaacaatta aagtcaaaga aaatttaaaa
     2281 cgtattgaac acgaacgcat cacggcaaga gtgacgctaa tggatgacga aaatgttatc
     2341 gctagtgacg tcggcgaggt gctatttaag aaagacgggt taagcgggat tgtaagtttt
     2401 attttaagta gttaccttga atgggctgtc gttagatatc caaaacgaaa ctattattta
     2461 acacttgatt taatgcctga atttagtgcg cgttacactg ccgaaattat taaaaccact
     2521 gatgacttag ataatgtctt tagccctaat attagtcact atctaaaagg tagaaatgaa
     2581 ccgttattag atcggatgaa ggctttaacc tttactccaa taccaaagaa caatccagaa
     2641 aacacaagtg cgatgcatgg tggaattaag ctttcaaaat taaatacgga ctttgaagta
     2701 cgcgaaagcc ccaatctctt tgctttaggt gaagtcataa acattgacgg aatatgcggt
     2761 ggctacaatt tagcgttcgc tttttactcc gcaaaagtcg taagtgacgc actcttacga
     2821 aaattgtaaa tatagcgctt ccactttata caatgtataa attactaatt aaaacctagc
     2881 aaaaaagggc tattttggtc aaattagtag acaattagta gttatttttc tcgcgaaatc
     2941 acaaaaaatg aacaaaattt cgttactgat taattactac tttcgagaac aaattaccga
     3001 taagtgacaa aagttgcaaa gtcacttttt ttgttttaat cttatggaca gaaattagaa
     3061 ataattttct tgagacacag agggagggtt ttttatggtc acacatatta agaaaagaga
     3121 cgggagatta caaaagttta atgctggtaa aattgctaat gctatcaatg cggcatatgg
     3181 gaacactaat gacgtgcagt cattacttta tgcagcggaa atcaccagta atttaaacac
     3241 gaattacgat gggacaatcg acattgaagt cgtccaagac gaagtcgaaa aattcttgat
     3301 gaagaaagat cccaacattg ctaaaaaata tattttatat agaaaagata gagaacggat
     3361 tcgtaacgct aaaagcaccg tttttaacta taaaaaactc gttgaagatt atttaaatat
     3421 tgccgactgg cgcgttaaag aaaattcaac cgtcaattat tctttaggtg gccttatctt
     3481 aagcaatagc ggcgctgtaa ccgcaaacta ttggttaagt gaagtgtatg acgaagaagt
     3541 agcaaatgcg caccgcagcg ccgccattca tattcatgac ctaagtatgc ttagtgctta
     3601 ctgtgcgggt tgggatattg aaaagattat tcgtgatggt gttggtggcg ttgaaggaaa
     3661 gatttcaagt aaaccagcga aacacttatt cacactcgct aatcaacttg ttaatttctt
     3721 aggcatcatg caaaacgaat gggctggggc tcaagctttt agtagctttg atacttacct
     3781 tgcaccattt gtaaaagctg ataacttaac ttacgctgaa gtaaaacaag ctattcaatc
     3841 atttgtgttt ggggtcaaca caccgagtcg ttgggggagt caagcaccgt ttagtaatat
     3901 tacactagac tggactgtcc ctagcgacct cgctgatcgt ccagcgattg tcggtgggaa
     3961 agatatgccc tttaaatata aagactgtca aaaagaaatg gatatgatta ataaagcgtt
     4021 ccttgaagta atgattgaag gtgacgctaa cggaagagga ttccagtatc ctatcccaac
     4081 atattccatt acgcgtgact tctcatggga agaaacagaa aacaataaac tattatttga
     4141 aatgacggca aagtatggta ctccttactt tagtaactat attaatagtg atatggaacc
     4201 aagtgatgtg cggagtatgt gctgtcgctt acggcttgac ttacgtgaat tacggaaaaa
     4261 aggtggcggc ttctttggtg ctggtagtaa tactggttca gttggtgttg tgacacttaa
     4321 catgccacgc ttagcctatc tctcccgtac tgaagttgaa ttctttaatg aacttgataa
     4381 attaatggac atcagtgccc gcagtttaaa agtaaagcgt gatgtcgtta gtaaattact
     4441 cgaagacggt ctcttcccat atacaaagcg ctacttaggt aactttgata atcacttctc
     4501 tacaattggt ttagtgggga tgaatgaagc gtgtttaaac gctagatggt taggaaaaga
     4561 cttaggcgat ccagaatcac aagcctttac gattaaagtg cttaatcata tgcgtgaaaa
     4621 acttagtgac tatcaagaag cttatggtga cctctataac ttagaagcaa caccagcgga
     4681 aagtacagcc taccgttttg ctaaacacga taaagtgcgt tatcccgata ttattactgc
     4741 taggttaggg gaagacggcg atcctttcta tacgaactca agtcacttac cagttggtta
     4801 taccgcaagt attagtgaag cgcttgatat tcaagatgaa atgcaaacac tttatacttc
     4861 agggactgtg ttccatactt tccttgggca aaagttgcca aactggcaaa ccgcagcccg
     4921 cttagtgcgg aaaattgctg aaaactatcg cttaccatac tttacaatgt caccaacata
     4981 ttccatctgt ccaacacatg gttacttaac tggtgaagtt gaaatttgtc cagaatgtaa
     5041 aagaacaaca gaagtgtatt cccggattac tggatactat cgtccgctta agaactggaa
     5101 tgacgggaaa aaagcggaat ttgaaaatcg tttagaatac gctgatttca ctattgaagg
     5161 tggcacagtg cataatccaa aatttaatgc accaaaagtt gaagtaaaag acacagcgga
     5221 cgcaccaatt aaagagcaaa agcctacccc acataaaggt gcaccaatca tcttcttaaa
     5281 caaacaaaat aatgactaaa aaaaagatac gcatagcggg atatgtaaac gactccttgg
     5341 ttgatggacc agggattcgt tttactattt ttacccaagg ttgtccctat cactgtttag
     5401 gttgtcataa tcctgataca tgggatccga agggtggaga agaagttgaa ataagtacgc
     5461 ttattgctaa atggcgcaag aactcgctgc ttgatggtat taccttaagt ggtggtgaac
     5521 cgcttttaca aaaagaagca ctcttaccgc ttattaacgc tgcgagaaaa gacggactta
     5581 atattacgat gtacactggt tcaacttatg aagagttaat tcacagaaac gatccacttg
     5641 tcaatgaaat cttaagtaaa gttgattact taattgatgg tccttttatt cttcatgaac
     5701 ttaatcttaa tttactctat cgtggcagta ataaccaacg gattattgat ttaagaaaaa
     5761 caacacctaa taacattgtc cttattaata caatgcatga ctttaagtaa agaaagccat
     5821 aatgaaaaag gagtgacaag agacgcgtca ctccttttgt ttataaagtg ttttttctat
     5881 tttctaaaca ttattttttc attgctaaac atgacggtaa gcaagtaaac tccgccggca
     5941 gttaaaagta agttagaaag cattgttaag ataaacggta caacgaggaa ttcaccacca
     6001 aagagtgaga gtaaaatatt aaccgagtta taaattggga ttaagtagag cgcgttattc
     6061 gttgccgcac taccaaacat cgtggtgacc cctaccgcca taattaccat catgagtggt
     6121 gctgctagtc cgctagcttc tttaatcgac ttagcgtacg cactgatgat gctatttaat
     6181 acaataaaga cgagcatcga gacaattaag acggcaaaga tagctaagta agtaccagcg
     6241 gtaaacgtcg ctagactaag ttcactcccc atcatttttg gtaagctaag cattactcca
     6301 aggaaactac ttgcagcgct gacaagggcg acgatactta aagctgtaac ttttccaagc
     6361 gctaaagtac tccttttcac tggtgtaact aagagggttg caatcgtacc gcgttctttt
     6421 tcgccagcaa tactttcagg agcaaccgcc attgcaccgg taaagataaa ggtaataagt
     6481 aagaagggga gcagcccttt aataattgta gtatcaatcc cgccttctgc cgaatgatta
     6541 atgggaacgg caacataagt ttggagggac gcatttaaga gcccagcata agtgctataa
     6601 gcatttaagc ttgggatcac tgattcatcg tagtgaaaag taacaactgg cggatcatta
     6661 ggatcattcg cgttatattt tgctaaaaaa tcagtgttat aaacaacata taagtcaagt
     6721 ttcttttccg caatcattgc ttcaaagtct tcaggagttt catcaaggaa cgtaactttt
     6781 ttaaacccga cttcaggatc agcgatcagt gtttcaatcg cgaggaaccg cgggtcattt
     6841 ggtctatttt cgacacggat gacgtattca tggtctttcc cttccattgt tgattgcatc
     6901 acatttccca taattgaata gagaatatac acgataacac caggaaggat taaggccgct
     6961 agcattcttt tatcagtgaa gaagcgcttt aattcttttt taatgacagt ccataagttt
     7021 ttcattaggc aaggtctcct ttcactttcg catattcaag gaagaagcgt tcctcaaggg
     7081 aattaggttc actaaatgtg ctcattggtt cttcaaagat taacgcccca tcaataatga
     7141 caccgacacg gtcacatatt ctttcgacga gtgagaagat gtgcgtagaa agaataacag
     7201 tctttcctgc ttctttcagc gcgacaagga aatcaatgac tgttttcgca gtaataacat
     7261 caaggccgtt tgtcggctca tcaaagataa tgaattttgg atcatgaacg acactgatga
     7321 caagtgaaac cttttgtttc atcccgctac taaggtcact tacttttact tctttaaatt
     7381 cactgatgcc gaatttatcg aaaagttctt gtttacgttt ttcaatcaca tcttcaggaa
     7441 caccgtgaag atgactaaag taatcaaata agtaattagg agtgaagaag tcgtctaact
     7501 ttaattcact cgttaaaaag ccaatttgac tgcgaacttt actttgttct ttactaatgt
     7561 cataaccatt aactttaact tcaccgttat cttttttaat taaggtcgag atgatgcgta
     7621 gtgttgttgt tttacctgcg ccatttggtc caagtaaacc atatatttct ccttcataaa
     7681 cattaaagga gaggtcatta accgcaacac gaactttcgt atttgttttt tcgatttttt
     7741 gttgtttttt cgaaagttta aaggtcttta ctaaattttt aacttctaag attggttgca
     7801 tagttcctcc tcatttcttt attatacata aagaatatat tggattttga cgttattaca
     7861 attatttacg agatttttac agaaattagg cgaagtaata ttgtatttat taatagtggg
     7921 attaattatt aaacgcttaa tttaattaat aaaataatga caacatattg acaatgtaat
     7981 gatataataa taaaatgaca ttgaatagga ggaattaaag tatgtccatg acgaatttaa
     8041 atattaggat ggataagaaa gtaaaagaag aagcagaagc gatatttcaa gaattgggac
     8101 tcaatatgac gactgctgtt aacatatttt tacggacgac aattcgtgaa aaaggcatcc
     8161 catttcattt aagtttaagt gtttcaaatg ttaccactaa agcggcaatt gaagaaggaa
     8221 gaaagattgc gcttactgat aatgttaagg ggtatgatac cgtttctgat ttaaaggcag
     8281 cattggaaga ttaatgttta aaatcaattt aaatttagta atctggtgtt taattgtagt
     8341 acgcttttta tcatagtttt aataattaaa tctttttgtt ataattgagt agcgcttcca
     8401 tagccaagtg gtaaggcaat tgactgcaac tcaatgaccg ttggttcaaa tccgactgga
     8461 agctccaaaa aaatctttgt ttggtaaggt aaaagcgact aaaacaacgt taaaacgccc
     8521 ttaaatgggc gttttttcat tatatggatt aaattagatg atttattttg tttgttaaat
     8581 ttgtgatcat atttataaaa gtatcaaaat tacatttccg ttacagtggt ttgaacctaa
     8641 aatactattt tccgttacag tggttcgaac ctaaataaaa ataaaaaaaa caatgtcttt
     8701 attaccatta ccaaccctta aattaattga tgaatgtaat aaaggataat ccagaatatc
     8761 tgtttccaat aaataaaatc ttttctagtc ttaattgtgc ttagttgctt
//
LOCUS       DAOSLP010000039         8189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_8159_length_8189_cov_8.824932, whole genome shotgun sequence.
ACCESSION   DAOSLP010000039 DAOSLP010000000
VERSION     DAOSLP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_8189_cov_8.824932"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..667)
                     /locus_tag="PK460_04165"
     CDS             complement(62..667)
                     /locus_tag="PK460_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOE54301.1"
                     /translation="MDSKNLYDFLIKVQSIAKIGLLYTTDPYAKKNYNEIGQMTLSLL
                     EDLQAVNLERNNYFKRDIYPTPNISVRAIIFNKEGQLLMVQEKEDGGFSLPGGWADLY
                     DSPTEAILKETTEEAGAKIKIMGIVAFLHRTPFKTPTSVPEYVLVFKAEFVEFIGEHD
                     HEILTVKWVDPYNLPPLSHKVTKHEVERMINAALHDKTIFD"
     gene            763..1527
                     /gene="pgeF"
                     /locus_tag="PK460_04170"
     CDS             763..1527
                     /gene="pgeF"
                     /locus_tag="PK460_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOE54302.1"
                     /translation="MYKWNDFKKITAVTSKRTGGFSTGDFSSLNLALHVGDDKETVFK
                     NRECFFGKKEVMMAPKNSVFVAQNHSTIVKKVTFKDAGRGFYDFNDGIKADALYTDEP
                     GLNLAIYHADCVPVFIYVPTHNIVGIIHAGEQGSVGNITGILVRKLFLEGVKPEEVYA
                     HIGPSASMGHRVITKERAFELAALGGDIIKAIKATEPQYFIDLPLLNTLQLRTLGVPL
                     SNITYSNICTYENNDEYYSYARNKVTGRNISFIRLN"
     gene            complement(1528..2004)
                     /gene="coaD"
                     /locus_tag="PK460_04175"
     CDS             complement(1528..2004)
                     /gene="coaD"
                     /locus_tag="PK460_04175"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOE54303.1"
                     /translation="MKLIAIYPGSFDPITNGHIDILNRALKIFDNVIVLVADNPAKKP
                     TFSVEERKAMIRESINGNPNVKVDSTTGLTIHYAEKVGAYALIRGLRAAIDFEYEFQI
                     NSANSFINSGVESVFLMAKQEFTFISSSTIKEMVSGGVDVSSLVPKPVLKRLEKLK"
     gene            complement(2001..2543)
                     /gene="rsmD"
                     /locus_tag="PK460_04180"
     CDS             complement(2001..2543)
                     /gene="rsmD"
                     /locus_tag="PK460_04180"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOE54304.1"
                     /translation="MLAKGRVIAGKYRHRHLIALFDEKTRTTKDRVKEAIFSAVGDYI
                     NGKTVLDLFAGTGALGIEAISRGAAHGIFCENDSKTYDVLTRNLQMIEEPYNLYFEDY
                     KSALAKIKPNSVSIVIIDPPYRYDIDEIINNVFKAKILTNSYTLVLETDKEYAGVINN
                     AKIKKYKYGLTHVTIIRGEL"
     gene            complement(2522..2896)
                     /locus_tag="PK460_04185"
     CDS             complement(2522..2896)
                     /locus_tag="PK460_04185"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOE54305.1"
                     /translation="MNQKLYELALDLHQNLEEHPSVLLLKEYEKAMLKDSTVKALIEQ
                     YDALQEEINSLLESCDLENKAIKAKCSTLYKLKYVLDNEPLVKRYNKQYNLVNKMYKN
                     ITEELTNAVGIKKEIKCLLKDE"
     gene            2989..3528
                     /gene="def"
                     /locus_tag="PK460_04190"
     CDS             2989..3528
                     /gene="def"
                     /locus_tag="PK460_04190"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016936264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOE54306.1"
                     /translation="MLKIFKDSDPLLRKRAEQVSLPLSDSDRELALAMLEHLKESQDP
                     LFAEEHNLRPGVGLAAPQVGESKQIIVVHIPRGKTERLTYVLANPIIISSSVRRAYLR
                     SGEGCLSVDEDHPGHVYRHYKIRVKAFDILNNEEVEINTFGYPAIVLQHEIDHLHGVL
                     FYDRINEENKVRYPDALSL"
     gene            3614..5296
                     /locus_tag="PK460_04195"
     CDS             3614..5296
                     /locus_tag="PK460_04195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOE54307.1"
                     /translation="MAINSKFEMPVSIYALGGLGEVGKNMYVVEDEKNILLFDAGVLF
                     PEQDLPGIDYVIPDFAYLRDNARKIKALFITHGHEDHIGAIPFLVQVVPVPVIYAPPL
                     AAALIRHKLQMMRIKTPVNIVEYDGDTVVKFGSMTVSFFAVTHSIPDCYGFCVDTEHG
                     RVVGTGDFKIDLTPIGRQMDLGKIAELGNEGVDLFLSDSTNAEHEGFTPSERTVISAI
                     NEIFANTSGRLIISTFSSNISRIQQIVEATVNYRRKIIIIGRSMERVVSASRDLGYIK
                     IPDASIVDVGKSSTLKPEETVVLCTGSQGEPMAALSRIADGTHKDISIIPGDTVVFSS
                     SPIPGNSVGIDRVVNQLTRLGATVLTNSILRNIHSSGHPSQEELKLMLRLVRPKYFMP
                     VHGEYRMLKLHAELAAKVGVPLENSFVLKNGETLELRNGKVTRGRSFPVADIYIDGNS
                     TEGMSKAVLTDRRQLNQDGMVAVLIAVDPKKNELLVPVEIYSRGFINWSISNLLPDAE
                     KHINKVLKRHYAEKVTTFGEVKNIVKDELEPFFYDKTGRKPMIIPVIMSKGI"
     gene            5298..5840
                     /locus_tag="PK460_04200"
     CDS             5298..5840
                     /locus_tag="PK460_04200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOE54308.1"
                     /translation="MFILASKSPRRQELFKLIIDDFAIIVPDIDENIPVSIKSDTAKI
                     ISKFKAYHIKAKYPEATILACDTIVVIDGELLGKPKDEDDARRMLTKLSGRKHHVISG
                     FTLVSPEFEINKNVVTEVYFNTLSPELIEAYIKSGSPFDKAGAYGIQDEEFKLVREIK
                     GSYYNVMGLPVEELRKFIKK"
     gene            complement(5817..6173)
                     /locus_tag="PK460_04205"
     CDS             complement(5817..6173)
                     /locus_tag="PK460_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HOE54309.1"
                     /translation="MTFSEFLQQYGVYLAFVVFLAIVFLIILILVIPRLPKKGTKSVE
                     PSISKLEIITLLGGEDNIKEIALRGSRLTVSLADKSLANIEELKKHGVDRVIVMQAKL
                     VLLVNKEVASLFDELA"
     gene            complement(6163..6969)
                     /locus_tag="PK460_04210"
     CDS             complement(6163..6969)
                     /locus_tag="PK460_04210"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003424.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOE54310.1"
                     /translation="MALKINVIVREDEKSLSVSKSLCELALQKGFLIDEVNPDFVFSV
                     GGDGTFLKAVHRYLTKVKLIKFVGIHTGSLGFFADFRPEDINNLLDNIIKEELNTNAY
                     RLVKAEITQNNKKRNFYAVNEVRIENVHHTLVLDVYLNDEHLEMYRGNGVLVATQLGS
                     TGYNKSIGGAVINRNLELLEYTKIAPIANSAFRPLVNPLIIGEKDVLVFNGKNANTYF
                     GYDAFTKKLSDIPFSVKVSLSNKKITIVHRQNRPYSRILNESFLGGKHDI"
     gene            7025..7603
                     /locus_tag="PK460_04215"
     CDS             7025..7603
                     /locus_tag="PK460_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOE54311.1"
                     /translation="MATSIEIEAKALLTKKDYNKVLKHFKLDVVDGYIQKNYYIDTEN
                     KDLRKLGLSLRVRRLNGYVISFKLPMAEGLLEKTQTLSREQFEDFETHGKFPEGDISD
                     FIESLYISPSDLKIIATLTTLRIEQEYEDLTLAIDENRYGELQDFELEMEATSMKKAQ
                     YLLKKVCEEVGIDYKENLVSKQKRAMDALSAV"
     gene            complement(7619..8065)
                     /gene="spx"
                     /locus_tag="PK460_04220"
     CDS             complement(7619..8065)
                     /gene="spx"
                     /locus_tag="PK460_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HOE54312.1"
                     /translation="MIKIYTSPSCSSCRKVKQWFDEQQIPYEEHNIFSASLSEAELKE
                     ILYKSENGTDDIISTRSKVIKEKNIDVNDLTMSELVQFIKDNPSVLKRPIMVDTNRIQ
                     VGYNSEEIMAFIPRERRAQMEAELMMGVRQQNEDTEICLDDDCPTK"
BASE COUNT         2604 a         1467 c         1411 g         2707 t
ORIGIN      
        1 atctccttta attttatttt ttattatagc atttatgcgc tccacgtggt acgagtatat
       61 gctaatcaaa aatagttttg tcatgaagcg cggcattgat cattcgttca acttcatgtt
      121 ttgtaacttt atgtgaaagt ggcggcaagt tataaggatc aacccatttt acggtaagta
      181 tttcatggtc atgttcccca ataaactcaa caaactcagc tttaaacact aaaacatatt
      241 ccggcacaga ggtaggagtt ttaaaaggtg tgcgatgcaa aaaggccaca atccccataa
      301 ttttaatttt tgctccagct tcttcagttg tttcttttaa gatggcttca gttgggctat
      361 catataaatc tgcccatcct ccaggcaacg aaaaaccccc gtcttccttt tcttgaacca
      421 ttaataattg gccttcttta ttaaaaatga tagcgcgaac gctaatattt ggtgttggat
      481 aaatatctct tttgaaataa ttatttcgct ctaaattcac tgcttgcaga tcttcaagta
      541 atgaaagcgt catttgtcca atttcattat aatttttctt tgcgtacggg tcggttgtat
      601 ataataatcc aatcttagcg attgattgta ctttaataag aaaatcataa aggtttttac
      661 tatccataat aaatttagta taacatacta gtttaaagtg atggcttact atgaataata
      721 atttaactac tttttatgta taataagaat aggaggcgcc ttatgtataa atggaatgat
      781 tttaaaaaga ttacagctgt tacttcaaaa cggactggcg ggttttcaac cggagatttt
      841 tcttcattaa atctagcact acacgttggt gatgataaag aaacagtttt taaaaataga
      901 gaatgctttt tcggcaaaaa agaagtgatg atggcaccta aaaatagtgt gtttgttgct
      961 caaaatcata gtacgatcgt taaaaaggta acttttaaag atgctggacg cggtttttat
     1021 gattttaatg atggaattaa agctgatgct ctctatactg atgaacctgg acttaatctt
     1081 gctatttatc acgctgattg tgtaccagtt tttatttatg tgccaacaca taacatcgta
     1141 gggattattc acgctggtga acaaggaagt gtcggcaata ttactggtat tctcgttcgt
     1201 aaattattcc ttgaaggtgt taaacctgaa gaagtttatg cacacattgg accaagcgca
     1261 agcatgggtc atcgtgtaat tacaaaagag cgtgcctttg aacttgctgc gttaggtggt
     1321 gatatcatca aggcaataaa ggcaactgaa cctcaatatt tcattgatct accactactt
     1381 aataccttgc agcttcgcac gcttggtgta ccacttagta atattactta cagcaatatt
     1441 tgtacatatg aaaataacga cgagtactac tcctatgcaa ggaataaagt aacaggtcgt
     1501 aatatttctt ttattcgttt aaactaatta ttttaatttt tctaatcgct ttaaaactgg
     1561 tttaggtacg aggctactta catcaacgcc tccgctaacc atctctttaa ttgtgctact
     1621 agaaataaat gtaaattctt gtttagccat taaaaagact gattccacgc cagaattaat
     1681 aaaactattt gcactattga tttggaactc atattcaaag tctatggcgg cgcgtaaccc
     1741 acgaatcagg gcgtatgccc ctactttttc tgcataatgt atagttaaac cagttgttga
     1801 atcaacttta acatttggat taccatttat tgattctcta atcatcgctt ttcgttcttc
     1861 caccgaaaat gttggctttt tagcgggatt gtctgcaact agaacaatga cattatcaaa
     1921 tatttttaac gctcgattca aaatatcaat atgcccattt gtaatcggat caaaactccc
     1981 cggatatata gctattaatt tcataattca cctctaatta tggtaacgtg tgtcaaccca
     2041 tatttatact tttttatttt agcattattt atcactcctg catattcttt atctgtttct
     2101 aatacaagag tgtaactatt tgttaatatt ttagctttga aaacattatt aataatttcg
     2161 tcaatgtcat aacggtaggg tgggtcaata ataacaatag aaacggaatt aggcttaatc
     2221 ttcgctaaag cactcttata atcttcaaaa tataaattat aaggctcttc aatcatttgt
     2281 aagtttctag ttaatacatc atatgtttta ctatcatttt cacagaaaat accatgagca
     2341 gcgccacggg agattgcttc aataccaagt gccccagtac cggcaaataa atcaagaact
     2401 gtcttgccgt ttatataatc acccactgct gaaaaaattg cttctttcac acggtctttt
     2461 gttgttcgtg ttttctcgtc aaaaagggca ataaggtggc gatgtcgata ctttccagca
     2521 attactcgtc ctttagcaag catttaatct ccttttttat tcccactgca tttgtcagtt
     2581 cttctgtgat atttttatac attttattaa caagattata ttgcttgttg tagcgtttta
     2641 ccagtggttc attatctaaa acatacttta atttataaag agtactgcat tttgctttaa
     2701 tcgctttatt ttctaaatca cacgactcaa gcaaactatt aatctcttct tgcaaggcgt
     2761 catattgctc aatcagtgcc ttaactgtgc tatcttttag cattgctttt tcatattctt
     2821 tcaaaagtaa aacacttgga tgctcttcaa gattctgatg taaatcaagc gctaattcat
     2881 atagtttttg gttcataaac taattataca agaaatgatg aattatcata tagcgttaat
     2941 actcacttgt taacacagat gtgttaaaat atttccggag ttagaagaat gctgaaaata
     3001 tttaaagata gtgatccctt attacgcaaa agagccgaac aagtttcgct cccactttca
     3061 gattctgatc gagaactcgc ccttgcaatg cttgagcatt taaaggaaag ccaagacccg
     3121 ctttttgctg aggaacataa cttacgacca ggagtaggtt tagctgctcc acaagttggg
     3181 gagagtaaac aaattattgt cgttcatatc ccccgtggta aaactgaaag attaacatat
     3241 gttctcgcaa atccgattat tatatcttct tctgtgcgcc gtgcatattt acgcagcggt
     3301 gaaggctgtt tatcagtgga cgaagaccat cctggtcatg tatatcgtca ttacaaaata
     3361 agagtaaaag cttttgatat cttaaacaac gaagaagtcg aaataaatac ttttggttat
     3421 cctgctatcg tattgcaaca tgagattgat catctccatg gtgtcttatt ttatgaccgg
     3481 attaatgagg aaaacaaagt tcgatatccg gacgctctct ctttgtagag acttttaaat
     3541 ttacatttaa ttattaattt ggcataatag gtaagatatt gatctatata tttaataatt
     3601 ctaggagata attatggcta ttaattcaaa atttgaaatg ccggtttcta tttatgccct
     3661 tggtggttta ggtgaggtcg gtaaaaacat gtatgttgtt gaagatgaaa aaaacatcct
     3721 actttttgac gctggtgtat tattccccga acaagattta cctggtattg attatgttat
     3781 tcctgatttt gcctatttac gcgataatgc acgcaaaata aaagcacttt ttattactca
     3841 cggtcacgaa gaccatattg gggcaatccc gtttctagtt caggttgtac cagtacctgt
     3901 tatttatgca cccccactag cagcagcgct cattcgtcat aaattacaaa tgatgcgtat
     3961 taagacaccc gttaacattg tcgaatacga tggcgataca gtagtaaaat ttggtagtat
     4021 gactgttagt ttctttgccg tgacacactc tatccctgat tgttacggat tttgtgttga
     4081 tactgaacat ggccgtgttg ttgggactgg cgacttcaag attgacctaa caccaattgg
     4141 gcgccaaatg gatttaggta aaattgctga acttggtaat gaaggtgttg atctcttttt
     4201 aagtgattcg acgaatgccg aacatgaagg ttttacccca agtgaaagaa ccgtcatcag
     4261 tgctattaat gaaatctttg caaacacaag tggacgacta attatttcta cattctcaag
     4321 taacatcagt cgtatccaac aaattgtcga agcaacagtt aattataggc gtaaaatcat
     4381 tattattggt cgttcaatgg aacgcgttgt ttctgcttct cgcgacttag gatatattaa
     4441 aattcctgac gctagtattg tcgatgtagg taagtcatca acactaaaac cagaagaaac
     4501 agtcgtgctt tgtactggtt cacaaggtga accgatggca gcactttcgc gtattgccga
     4561 tggtacccat aaagatatta gtattattcc aggtgatact gtcgtattta gtagttcacc
     4621 aattcccggt aatagcgtcg ggattgaccg cgttgttaac caattaacta gacttggagc
     4681 gacagtttta acaaacagta ttttacgtaa tattcatagt tctggtcacc cttcgcaaga
     4741 agaattaaaa ctaatgttgc gccttgtgcg tccaaaatat ttcatgccag ttcacggtga
     4801 atatcggatg ctgaaactac atgccgaact tgcagcaaag gtcggtgttc cccttgaaaa
     4861 ctctttcgta ctaaaaaacg gtgaaacttt agaattacga aatggcaaag taacacgtgg
     4921 tcgttctttc ccagtcgccg atatttatat tgatggtaat tctaccgaag gcatgagtaa
     4981 agcggtccta actgacagac gacaattaaa tcaagacggg atggtcgccg tgcttattgc
     5041 tgttgatcca aagaaaaatg aacttttagt gcctgttgaa atttatagtc gcggctttat
     5101 taattggagt atttctaatt tattaccaga tgccgaaaag catattaaca aagtattaaa
     5161 gcgccactat gctgaaaaag taacaacttt tggtgaagtt aaaaacatcg ttaaggatga
     5221 gctcgaacca ttcttttatg acaaaactgg tcgtaaacca atgattattc ccgtcatcat
     5281 gagtaaagga atttagtatg ttcatcctag cgtcaaagtc accgcgccgc caagaattat
     5341 ttaaattaat tatcgatgat tttgcaatta tcgtccccga tattgatgaa aatattccgg
     5401 ttagcattaa aagcgacacc gctaaaataa tttctaaatt taaggcttat catattaagg
     5461 caaagtaccc tgaagctact attttagcgt gcgatacaat tgtggtcatt gacggtgaac
     5521 tacttggtaa accaaaagac gaagatgacg ctagaaggat gctcactaaa ttaagtgggc
     5581 gcaaacacca tgttatcagt ggcttcacac tcgttagtcc cgaatttgaa ataaacaaaa
     5641 atgttgttac tgaagtatat ttcaacactt taagtcctga gcttattgaa gcatatataa
     5701 aaagcggctc accatttgat aaagcgggag cctatggtat tcaagacgaa gaatttaagc
     5761 ttgtccgtga aattaaaggt agttactata atgttatggg gctgccggta gaagaattac
     5821 gcaagttcat caaaaagtga tgcgacttct ttattcacga gtaaaacaag ttttgcttgc
     5881 ataacaatta cacggtcgac accgtgtttt tttaattctt caatgtttgc aagcgactta
     5941 tccgcaagtg atactgttaa acgcgaaccg cgtaacgcta tttctttaat attatcttca
     6001 ccacctaaaa gtgtaataat ttctaatttt gaaatgcttg gttcaacact ttttgtaccc
     6061 tttttaggta aacgcggaat aactaaaatt aatatgatta aaaagacaat tgctaaaaat
     6121 acaacaaaag ctaaatacac accatattgc tgcaaaaact cactaaatgt catgtttacc
     6181 tcctaaaaaa gattcattta atatacggga ataaggacgg ttttgacgat gaactattgt
     6241 tattttttta ttactaagac tcactttaac actaaaaggt atgtcactta atttcttggt
     6301 aaaagcgtca taaccaaaat atgtattggc atttttcccg ttgaacacta acacatcttt
     6361 ttcaccaata ataagcgggt taacaagtgg ccgaaatgca ctgtttgcaa tcggtgctat
     6421 ttttgtgtat tcaagtaatt ctaaattacg attaatcaca gcgccgccaa ttgatttatt
     6481 gtaacccgta ctgccaagct gcgtagcaac taacacccca ttaccacgat acatttctag
     6541 atgttcatca tttaaataaa catcaagcac aagtgtgtga tgaacatttt caatccgcac
     6601 ttcgttgacc gcataaaagt tacgcttctt attattttgg gtaatttctg cttttactaa
     6661 gcgataagca ttagtgttta gttcttcttt aataatgtta tctaaaagat tgttaatatc
     6721 ttccggacgg aaatcagcaa agaatccaag tgaaccagta tgaatgccaa caaacttaat
     6781 aagtttaact ttagtgagat agcgatggac tgcttttaaa aatgttccgt cacccccaac
     6841 actaaagaca aagtcaggat taacttcatc aatcaaaaag cctttttgta gtgcaagttc
     6901 acataaactt ttacttacac taagactctt ttcatcttca cgcacaatta cattaatttt
     6961 aagggccata actttataaa tcttcgttta tattgtatca tattgataga aagaggtaat
     7021 tactatggcc acgagtattg aaattgaagc aaaagcacta ctgacgaaga aagattataa
     7081 caaagtatta aaacacttta agctagatgt tgttgatggt tatattcaga aaaattatta
     7141 tattgatact gaaaataaag atttgcgcaa actaggatta agtctccgtg ttcgccgctt
     7201 aaatggatac gttatttctt ttaaactacc gatggcggaa ggattacttg aaaaaacgca
     7261 aacattaagc cgtgaacaat ttgaagattt tgaaactcat ggtaaattcc ctgaaggcga
     7321 tattagtgac tttatcgaaa gtttatatat tagtccaagt gatttaaaga ttattgctac
     7381 tttaacaaca ttacgcattg agcaagaata tgaagaccta actttagcga tcgatgaaaa
     7441 tcgttatggt gaactacaag actttgaact tgagatggaa gcaacatcga tgaaaaaagc
     7501 ccaatatctg ctcaaaaaag tatgtgaaga agttgggatt gattataaag aaaaccttgt
     7561 aagcaaacaa aaaagagcaa tggatgctct ttctgcagtt taataaaata aataaacctt
     7621 atttagtagg acaatcgtca tcaagacata tttcggtgtc ttcattttgt tgacggacac
     7681 ccatcattaa ttccgcttcc atttgggcgc gtctttcacg tgggataaat gccatgattt
     7741 cttcactatt gtagccaact tgaatccgat tagtatcaac cataatcggt ctttttaata
     7801 cggacggatt atctttaata aattgcacta attcactcat tgttaagtca ttaacatcaa
     7861 tatttttttc tttaataact ttactacgag tgctgataat atcatcagtg ccgttttccg
     7921 acttatataa gatttcttta agttcggctt cactaagtga agcagagaag atgttatgtt
     7981 cttcatatgg gatttgttgc tcatcaaacc attgcttaac cttacgacat gacgaacaac
     8041 ttggtgatgt ataaatttta atcatattca ggacctcctt gttccgcgta ttaatataac
     8101 ttatttacta tgtaaaagca atacctgaaa tgaattcgtt aaaggaaaag gttaaattta
     8161 catccttctt gtcttcatta taatgttta
//
LOCUS       DAOSLP010000040         7929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_8461_length_7929_cov_8.898400, whole genome shotgun sequence.
ACCESSION   DAOSLP010000040 DAOSLP010000000
VERSION     DAOSLP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8461_length_7929_cov_8.898400"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..1969
                     /locus_tag="PK460_04225"
     CDS             431..1969
                     /locus_tag="PK460_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOE54313.1"
                     /translation="MKFLKNLKEALISSLPLLAVIILVAGIIFPVKTNTEIIQLVVGY
                     TGVVIGQAIFLTGLDYSVLPIGNLIGSSIVKLNKLVFVLMFAFLFSLLAAVAEPGITV
                     LAAQVGGIHPRINSTLFIWVTSFGMGIFTALAVYRLLKGVSMKKIFFFFYLAVIIIIF
                     FVPSQFQALGFDASGAATGAISVPFILALGVGVSHTVSRNKSAEDSFGIVGIAAIGPI
                     ITVYLYGLIVGNSDTLNVYTPGENSDLLAVMLGNLLPVALAVFPLMLIFLIFQAFFIK
                     LPRRKIGQIIIASLVVFVGLFIFIAATDFGLAFAGKYIGEQFMMSEKLWWLLLPLAFV
                     LGFAITLSEPSVVVFCEQVDELTSGSISKNTIKMALGISVGLATFFAVIKTILDINIL
                     WFIVPFYVITIGLIYFTPTLFVGLAFDGGTVSAGAITSAFLTPVTLGASQALGHTNIL
                     TSGLGILAFISVMPRIVVQILGIIYNYRLKKATPPVYEDISYDATTLSETETEIQEEA
                     TNDA"
     gene            1959..2267
                     /locus_tag="PK460_04230"
     CDS             1959..2267
                     /locus_tag="PK460_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54314.1"
                     /translation="MTHKEEWVVMVIITRRKYKAELVKMLYDHEAAMVHQTYAKGSAK
                     ASFLRALGLTQEQNKLFLLGFVKTDQVPAIFSVLEYKYKFKEPNSGFAFTFPLEKISC
                     "
     gene            2280..2588
                     /locus_tag="PK460_04235"
     CDS             2280..2588
                     /locus_tag="PK460_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOE54315.1"
                     /translation="MTESMKALFIIVNAGFSEQIVEVVQNHGARGATIIPARGTGKKF
                     VKVLGIQYEPEREILLSVVEEDIALKITADVKENYGKNTPTNGLCFILPVADYMFKNE
                     "
     gene            2640..6215
                     /locus_tag="PK460_04240"
     CDS             2640..6215
                     /locus_tag="PK460_04240"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOE54316.1"
                     /translation="MGNFIRKHIDTLLLVLNVLVFVLFTLVYPFVTFKHTFLNALFYI
                     LLSAPLITFLVLRFLKYDRKTEESIFYYRRGFNLLALINNYLFMMSAESIFTLIKGHR
                     APLFIIYLIFFALMWLINIFALILELGYLDQLLPLREYATDTAKEETIALAKAAKFKR
                     FEEKVSTMYFFIALVAVLAVIFFIYPLVTEYFTIPLGGDFTQQQIPFYTNGYDDWWRF
                     LKTGEFPLWDSNTFLGVNNIGSNSFYYSMNPFFLPILIFPRELIPQGISILMIGKFVL
                     AAVTMRLYLKYMGVNEKEARFFAVIYAFSGWNTYYLWFNHFMEVAVIFPLIFLGIEKL
                     IKEKVMWVLILSLGLMGFANYFFLMTVGVIGVLYAGFRYFQQLPKVEPSIIGLGIIAF
                     ALGLLLSSVVLFPSLVIALGSDRATDATYLEKLLDLFKNVNSISLKEQRRQIWHLITK
                     WEPYTMEGATPSTQYSYKKFYPLISYYFPVLSNRSVALLNTSNYDNTISSLFSFSPVI
                     ILFIPSLVVSAKKKKVSHFVALAFFLFALFTPFFYHAFHGFTKDYGRWQLIVTFSLIT
                     YVAMSLPEVKAEKPYLLDISIIFNLIMMGITAKVAFSFENKSGFSYMYQREYVIYYQA
                     IMLFISYLVLRLARNTKHEYYLRNAFVFFEIAVMGTITMIGHGFISYKDSVSGGYQNY
                     KDDLTTIREIKALDGSYYRIFNTRAYRGNDNLPMRENYNGLSAFHSLYNFELMPFNKW
                     SKINYNHKGWSLGVFEKRSMLNDFLQVKYYMLNNTNHVIYWSNAAPSVAPYRNVPNTY
                     EFMEELSTDKRFVYKMKEGFTLGFGVDNVISYENRLSDGTYFDLIEKDRALSPIQNEE
                     LYLTSALLSYEDHYEVTRTYSDLVSHDINAFPHQHYTRLSSTNGGLKVKYYEFTGRRY
                     PYEAQTRTLGEVVLPTPQWVSENGNEIISSLVPTELNGAHHVIEYTKKDGTNFVNGVN
                     ELGEQIGGEIIMRLPLSYYNSSTLYYNFRYRVFLYDENYELITFDDHHTLPSSWTDWK
                     TLRSFHTPKAVSKIALVPIGSRVTPPYVHELFTYSQDALESIRQTSITNSLTDVKVTT
                     NTATFKTNYPTRKFVVTTIPYDKGWRVTNENNIKIPVYRVQGGFVGFVSDRGEMSYTM
                     SFRPQYFNMGLVLTFSAGIATLLLELNVYFFKKYKKKKAEENINV"
     gene            complement(6205..7047)
                     /gene="rsmI"
                     /locus_tag="PK460_04245"
     CDS             complement(6205..7047)
                     /gene="rsmI"
                     /locus_tag="PK460_04245"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOE54317.1"
                     /translation="MKRNKSFSGTPFYLVATPIGNLEEFSPRAVNVLRSVDFICAEDT
                     RTSGVLLNHFGIKKELISVHKFNEQSKLELILNKLKNGEKGAFISDAGYPLISDPGHP
                     LVKALIANDFSVSVINGPSALLPALIGSALDTETFTFIGFLKGNNKDVLNTLKRYKSY
                     KDTLIIYEAPHRVVKTIATIYTVFAERKLVIARELSKLHEEYIYTSLAEFSANPPTDL
                     KGEIVLVIEGNKDEENNDLADLLDKVELLISNENMSVKKATSVVALLYNVSKNKLYNA
                     YLKR"
     gene            complement(7034..7867)
                     /gene="ricT"
                     /locus_tag="PK460_04250"
     CDS             complement(7034..7867)
                     /gene="ricT"
                     /locus_tag="PK460_04250"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOE54318.1"
                     /translation="MNDNNERYFISIIYNDGGKSYCFGTNESSLDIGDYVLVESSQGI
                     AVAKVVATPQTMDKYSSPLELKDIVRRANEHEIRQHQDNLKDAELAMAFCQKQVERLG
                     LKMNLLTSEYTFDRSKINITYSAEERVDFRELVKILGSHLKTRVELRQIGSRDRAKLI
                     GGLGQCGFPLCCHTFLTEFDGISINRAKNQMLPLNIPKLSGHCGKLLCCLKYEDDYYT
                     EAKKEFPNVGSTYTRGNTLYQLTSFNIVNRTLRLDYEDGFVIVPLDELKRDYKVKHEK
                     K"
BASE COUNT         2434 a         1336 c         1446 g         2713 t
ORIGIN      
        1 ctttttgata tatatttact atttaacttg attaagaata gttaatctcc tttcttatat
       61 atgtaaaaaa aacgaaaaca atttaaagtt tctttgtgtg ttttaattat cgctatataa
      121 ccaccccctt ttacgtcacc aaaaaggttc gtaaaatatt agtattcact acaaatgcag
      181 taaatagggg gctaaaagtc ttgaataaca acttttagaa gtctctttta tgataaagag
      241 aataacgcta aaagtcaaat ataaccggaa ttacttttca atattaaaat cgtaaagtaa
      301 tgatagtacg cattatttaa ttattaatgt aaaaaagttg taaggagggc agttataagt
      361 taatgccatc ttaaaatagt cgtaatatgt tatacttaca aagtataaaa aagaggaagg
      421 gtgtaaatat atgaagttct taaaaaacct taaagaagca cttatctcaa gtttacccct
      481 gcttgccgtt attatcttag tagcgggaat tattttccct gtcaaaacta atactgagat
      541 aattcaacta gtggtaggat ataccggtgt tgttattgga caagcgattt ttcttactgg
      601 tctagattat agtgtcttac caattgggaa cttgataggt agttctatcg ttaaactaaa
      661 taaattagtg ttcgtcttga tgtttgcgtt tttatttagt ttactcgctg ctgtggcgga
      721 accaggcatt actgttttag ctgcacaagt tggtggtatt cacccacgta taaatagcac
      781 actctttatt tgggtaacta gttttggtat gggaatattt acggccttag cggtatatcg
      841 tttgttaaaa ggtgtaagta tgaaaaaaat cttctttttc ttttatttag cagttatcat
      901 tattattttc tttgttccaa gtcaattcca agcccttggc tttgacgcta gtggggcggc
      961 aacaggtgcg attagtgttc cctttatttt agcgttaggt gtaggtgttt cccatactgt
     1021 tagtcgcaat aaaagtgcag aagatagttt tgggattgtt ggtattgcgg caattggtcc
     1081 aattattact gtttacttat atggattaat cgttggtaat agtgacacac ttaatgtata
     1141 tactcccggt gaaaatagtg atttactagc ggtaatgctt gggaatttat tgccagtcgc
     1201 ccttgcggtc tttccgctta tgttaatttt tcttattttc caagcattct ttattaagtt
     1261 gccgcgaaga aaaattggtc aaataataat tgcatcactt gttgtttttg ttgggttatt
     1321 tatttttatt gcagcgacag actttgggtt agcgtttgct ggtaaatata ttggcgaaca
     1381 atttatgatg agtgaaaaac tgtggtggct acttttacca ctagcgtttg tacttggctt
     1441 tgcaattact ttaagcgaac caagtgttgt tgtattctgt gagcaggttg atgaattaac
     1501 tagcggaagc atttctaaaa atacaattaa aatggcttta ggtatttccg ttggcttagc
     1561 gacattcttt gcagttatta aaacaatttt agatattaat atcttatggt ttatcgtacc
     1621 tttttatgta attacaattg gtttaattta ctttacacca acattattcg ttggtttagc
     1681 ctttgatggc ggtacggtaa gtgcgggcgc aattacgagt gcctttttaa ccccggtgac
     1741 actcggggct agtcaagctt tagggcacac taatatatta acctcaggct taggtatttt
     1801 agcctttatc agtgtgatgc cgcgtatcgt tgtgcaaata cttggcataa tttataacta
     1861 tcgtttgaag aaagcaacac caccagtgta tgaagatatt agttatgatg cgacaacgtt
     1921 aagcgaaaca gaaacagaaa tacaagagga ggccacaaat gacgcataaa gaagaatggg
     1981 ttgtgatggt tattattact cgtcgcaaat ataaagcgga gctcgtaaaa atgctctatg
     2041 accatgaagc agcaatggtt catcaaacat atgcgaaggg gagcgctaaa gctagtttct
     2101 tacgggcact tggcctaact caagaacaaa ataagctctt tttgctagga tttgtcaaga
     2161 ctgatcaagt tccagcaatc tttagtgtat tagagtataa atacaaattt aaagaaccaa
     2221 attctgggtt tgcttttaca ttcccacttg aaaagataag ttgctaggag gacattctta
     2281 tgactgaaag tatgaaagct ttatttatta tagttaacgc gggctttagt gaacaaatcg
     2341 ttgaagtcgt gcaaaatcat ggcgctagag gtgctactat tatccccgcg cgtggcactg
     2401 gcaagaagtt tgtcaaagta cttggtattc aatatgaacc agagcgcgaa atcttactta
     2461 gtgtcgtgga agaagatatc gcgcttaaga ttactgctga tgtgaaagaa aactacggta
     2521 aaaacacacc aactaatggg ttatgcttta ttttgcctgt tgctgattac atgtttaaaa
     2581 atgagtaaat aatagtttga taaccattaa tcttcaattt attttataat gataatacta
     2641 tgggaaactt tattcgtaaa cacattgata ctttactcct agttttaaat gtcttagtct
     2701 ttgttttatt tacgcttgtt tacccattcg tgaccttcaa acataccttc ttaaatgcac
     2761 tattctatat tttacttagt gcaccgctta ttactttctt agtgcttagg ttccttaaat
     2821 atgaccgcaa gactgaagaa agtatttttt attacaggcg tggcttcaac cttttagcgc
     2881 ttattaataa ctatttattt atgatgagtg ctgagagtat ttttacgctt attaaaggtc
     2941 atagagcgcc gttatttatt atttatttaa tattcttcgc tttaatgtgg ttaattaata
     3001 tctttgcact aatccttgaa cttggttact tagatcaact tttaccgctt cgagaatatg
     3061 cgactgacac cgcgaaagaa gaaacgatcg cattagcgaa agctgcaaag tttaaacgct
     3121 ttgaagaaaa agtttcaaca atgtacttct ttattgcgct tgtggcagta ctcgcggtta
     3181 ttttctttat ttatccactg gtgactgaat actttacgat tcctttaggt ggtgacttta
     3241 cacagcaaca aatccctttt tacacgaatg ggtatgatga ctggtggcgc ttcttaaaga
     3301 ccggtgaatt tccgctgtgg gatagtaata cctttttagg tgttaataac attggttcaa
     3361 atagttttta ctattcaatg aatcccttct tcttaccaat tcttattttc ccgcgtgaac
     3421 ttatcccaca agggattagt attttaatga ttggtaagtt tgtactagcg gcagtaacaa
     3481 tgcgtttata tttaaaatat atgggcgtta atgaaaaaga agcacgattc tttgctgtaa
     3541 tctatgcatt tagtggctgg aatacatatt acttatggtt taaccacttc atggaagttg
     3601 ccgttatttt cccgcttatt ttccttggta ttgaaaaatt aattaaagaa aaggttatgt
     3661 gggtcctaat tttgtcatta ggcttaatgg gttttgctaa ttacttcttc ttaatgactg
     3721 ttggggtcat tggggtgctc tacgctggtt ttcgctactt tcaacaatta ccaaaggttg
     3781 aaccaagtat tattggatta ggtattattg cttttgcttt aggattacta ttaagtagtg
     3841 tagtgttatt cccgagttta gtcattgctt taggtagtga ccgagcgaca gacgccactt
     3901 atttagaaaa attactagac ttatttaaaa atgttaatag tatcagtctt aaagaacagc
     3961 gccgccaaat ttggcatcta attactaaat gggagcctta caccatggaa ggcgctactc
     4021 cgagtacaca gtatagttat aaaaagtttt atccgcttat ttcttattac ttcccagtat
     4081 taagtaatag gagtgtcgct ctacttaata catcaaatta cgataacacg atttcaagtt
     4141 tgttttcgtt tagcccagtt attattttat ttatcccttc attagtcgta tccgcgaaaa
     4201 agaaaaaggt tagtcacttt gtagcacttg cgtttttctt gtttgcttta tttacgccct
     4261 tcttctatca cgctttccac gggtttacca aggattatgg tagatggcaa ctaattgtaa
     4321 cttttagttt aataacttat gttgcaatgt cgcttcctga agtgaaggca gaaaagcctt
     4381 atcttttaga tataagtatt atctttaact taattatgat gggaataacc gctaaagtag
     4441 cgttctcttt tgaaaataag agcggcttta gttatatgta tcaaagggaa tatgtaattt
     4501 actaccaagc aataatgctt tttattagtt atttagtcct tcgtttagcg cgtaacacaa
     4561 aacacgaata ttatttacgc aatgcttttg tgttttttga aatagcggta atggggacga
     4621 taacaatgat tgggcacggc tttattagct ataaagatag tgtgtcaggt ggttaccaaa
     4681 attataaaga tgatttaaca acgattagag aaattaaagc cttagatggt tcttattacc
     4741 gaatttttaa cactcgtgcc tatcgtggta atgataattt accaatgcgt gagaattata
     4801 acgggttaag tgccttccat agtctttata actttgaact gatgccattt aacaaatggt
     4861 caaaaattaa ctataaccat aagggctgga gtttaggtgt ctttgaaaaa cgtagtatgc
     4921 taaatgactt cttacaagtt aagtactata tgcttaataa tacgaaccat gttatatatt
     4981 ggagtaatgc tgcgccaagc gtagcgcctt accgtaatgt tccaaacact tatgaattta
     5041 tggaagagtt aagcactgat aaacgctttg tctacaagat gaaagaaggt tttacgcttg
     5101 gttttggagt tgataatgtt ataagttatg aaaataggct gagtgacggt acttattttg
     5161 acttaattga aaaagatcgc gccctttccc ctattcaaaa tgaagagctc tatttaacaa
     5221 gtgcgcttct tagttacgaa gatcattatg aagtaacaag gacttatagt gatttagtga
     5281 gtcatgatat taacgctttc ccgcatcaac attacacgcg gcttagtagc acaaacgggg
     5341 gcttaaaggt aaagtattac gaatttactg gccgccgcta tccctatgaa gcacaaacga
     5401 gaacattagg tgaagtcgtg ctaccgacac cccaatgggt cagtgaaaac gggaacgaaa
     5461 taataagtag cttagtgcca acagaattaa atggggcaca tcatgtcatt gaatatacga
     5521 agaaagatgg cacaaatttt gtgaatggtg taaatgaact aggcgaacaa attggcggcg
     5581 aaataattat gcgattacct ctttcttatt acaacagcag tacactttac tataactttc
     5641 gttatcgggt gtttttatat gatgaaaatt atgagttaat tacttttgat gatcatcata
     5701 ctttaccgag ttcatggaca gattggaaaa ctttacggag tttccacaca ccaaaagcgg
     5761 tatctaaaat tgcgctagtg ccaattggca gtcgtgttac tccgccatat gttcatgaat
     5821 tatttaccta ttcacaagat gcactagaat ctataagaca aacatcaatc actaattcac
     5881 taactgatgt taaggtcaca acgaataccg cgacatttaa aactaattat ccgacgcgca
     5941 agtttgttgt taccacaatt ccttacgata agggatggcg tgtcactaat gaaaacaata
     6001 taaaaatacc cgtttatcgg gtacaaggtg gatttgttgg ttttgttagc gatcggggtg
     6061 aaatgagtta tacaatgtcg tttagaccgc aatactttaa tatgggttta gtgcttactt
     6121 tctctgcagg tattgctaca ttattactag aactaaatgt gtacttcttt aagaaatata
     6181 agaagaaaaa agccgaagaa aatattaacg tttaagataa gcgttatata gtttattttt
     6241 agaaacatta tagagcaagg cgacgacact agtcgccttt ttaacactca tattttcatt
     6301 actaattaat agttctactt tatctaaaag gtccgctaag tcattatttt cttcatcttt
     6361 attgccttcg atgactaaaa cgatttcacc ttttaaatcc gtcggtggat tagcactaaa
     6421 ttccgctagt gatgtataaa tatattcttc atgcaattta cttaattcac gcgcaattac
     6481 aagctttctt tcggcaaaaa ccgtgtaaat cgtcgctatt gtcttaacaa cacgatgcgg
     6541 agcttcgtaa attattaatg tgtctttgta agatttataa cgctttaaag tgttgagcac
     6601 atctttatta ttacctttta aaaagccgat gaatgtaaat gtttcagtat ctaaggcgct
     6661 accaatcagc gctggtaaaa gtgcactagg accattaata acactaacac taaaatcgtt
     6721 agcgattaac gctttaacta acgggtgtcc tggatcacta ataagcggat aaccggcatc
     6781 actaataaag gcgccttttt ctccgttttt taatttattt aagattaact ctaatttact
     6841 ttgctcatta aatttatgga cactaataag ttccttttta ataccgaaat gatttaaaag
     6901 aacaccgctt gttctggtat cttcagcgca aataaaatca acgcttctta gcacattaac
     6961 agcgcgcggc gaaaactctt ctaaattgcc aattggcgta gccactaaat aaaatggtgt
     7021 accgctaaaa gatttatttc ttttcatgtt taactttata atcccttttt aattcatcta
     7081 agggcacaat cacaaaacca tcttcataat caagacgaag agtacggtta acgatattaa
     7141 atgatgttag ttgatacaaa gtgttaccgc gagtgtaagt cgaaccaaca ttagggaatt
     7201 ctttttttgc ttccgtataa taatcatctt catactttaa acaacaaagt aatttaccac
     7261 agtggccact taattttgga atgttaagcg gtaacatttg gtttttagca cgattaatcg
     7321 aaataccatc aaactctgtt aagaaagtat ggcaacaaag cgggaagccg cattgcccaa
     7381 gaccaccaat tagtttagcg cggtctctac taccaatttg gcgtagttct actctagttt
     7441 ttaagtgtga accaagaatt ttcacaagct ctctaaagtc aacgcgttct tcagcggagt
     7501 aagtaatatt aatcttacta cgatcaaaag tatattccga agtaagtaaa ttcattttaa
     7561 gaccaagccg ttcgacttgc ttttgacaaa aggccattgc aagttcagca tcttttagat
     7621 tatcttgatg ttgacggatt tcatgctcat tagcgcgtct aacaatatcc tttaactcta
     7681 atgggctact atacttatcc attgtttggg gcgttgccac tactttagca acggctatac
     7741 cttgactgct ttctactaaa acataatcgc ctatatctaa agaactttca tttgttccaa
     7801 aacaataaga cttgccacca tcgttatata taatacttat aaagtaacgt tcattgttat
     7861 cattcatctg ttgtttcctt taccattttg attaataatt catccacgag taagccagta
     7921 ttaacataa
//
LOCUS       DAOSLP010000041         7364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_9246_length_7364_cov_7.968806, whole genome shotgun sequence.
ACCESSION   DAOSLP010000041 DAOSLP010000000
VERSION     DAOSLP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9246_length_7364_cov_7.968806"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..877)
                     /locus_tag="PK460_04255"
     CDS             complement(146..877)
                     /locus_tag="PK460_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54319.1"
                     /translation="MQNENKTQKFQINKTFIIKLIVYLLGIILVGFGVQIIIYTNVGT
                     APIDAMNYYLARIYLQLFNNSDFSKLKTTIGVASLAFGTLTVLVLFIITKNKALIFTW
                     LNIFIVSLVISIWGLLFDTFNPLNTTFFIRALVALLGILIISFGVFLVIVTEFPAGPP
                     EEIMRLINKKTNNLFVAKLLAETFYLVLACISMIISVTIIDGNGVEFTQVGIFTIVTL
                     ILTSVFVSLFDLAYRKLVVKKVESA"
     gene            complement(970..3156)
                     /locus_tag="PK460_04260"
     CDS             complement(970..3156)
                     /locus_tag="PK460_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE54320.1"
                     /translation="MRILELNIDSFGKLENYNLKFDQKLTIINEDNSFGKTTIAQFIK
                     AMFFGMKTAKGKGKRLSLPRFKYLPWGKTTYGGNLTFVYNGRTYTVTRTFGESLSNDT
                     IAVKDLARNVNIDELNGIKITRDNLGDIIFGVNSESFEKLNFIQQLEVLYSSDEKIHD
                     DINVKLRGLFGHTTEDSDYTSAFNILDDAIKELSPGNQRRNSLYNKTDRELNATNNLV
                     FEAENAVNLISENSDELVALKRQKAENDKLKTKLENDLKLVANKKILEKDLKDYNALN
                     DEINNLNLQIEENKKLFAGNKVEDTNLVYLEELSTDLSAKESEFNKFKDEAQTEINSI
                     NAALNTLESTNVEVLFIKRAHKETSEEITKLENEVTLLNANIKRFRFLDIFLSIITLS
                     IYFFVLKSKNNKLRKEIALLNKTIDELNVKLENYAAELATMTSTESDIKLKQKLDEWT
                     QKVNQKQKELEAFKEHVFDVFRKYSVKVNSVSDVQVAYFLINSHYEKHLALNKKLNDV
                     NFKLKNYDIKKINSDLNSFALLDEEELNRNKSNVDIKNDELIKKITALEKQIEANEDL
                     ASNLENYKFERDELRTTMQNYEQKKELLTIARTLLEHANQQLAARYLTPLQNNVNDLL
                     TKIKLEGYSVMFDGNSEMKLKDLDTNEYYDFAYYSAGSQDLISVLVRVALIDLVYQDL
                     KPFIILDDTFVNFDDKRIKLVRPLLEKLSQDNQIIYFTCSSSRDLR"
     gene            complement(3153..4214)
                     /locus_tag="PK460_04265"
     CDS             complement(3153..4214)
                     /locus_tag="PK460_04265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOE54321.1"
                     /translation="MKIIHTADIHIDSKMNRYGGDKTERRIREIKNAFFRMIDYGVKH
                     NVKVILIAGDLFDNKEVKLATLREVLTKITSAPTIDFIYLAGNHDDPAVFNHEKLAEL
                     PSNLKIVTKENNTFLYDDVCIKAYDNYADEDYTKTLIFDPNKYNILMLHGTEAEIPLN
                     NLKGKNIDYLALGDIHIPDIAAKRLDARGIYAYSGVLEPRGFDEVGERGFFVLEIQKN
                     KLIRREFIKNNSRTYHIIDVDISALNNTLEIELAIKKATNKIPSDDIVRLKLTGKYDY
                     ELIKDINALREYLESKFYYAELIDDSKVNLNTIDFANEVSLRGEFFRLIEASNYSPEV
                     KEELLKLGLNALKGRKIDL"
     gene            complement(4376..5710)
                     /gene="mgtE"
                     /locus_tag="PK460_04270"
     CDS             complement(4376..5710)
                     /gene="mgtE"
                     /locus_tag="PK460_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOE54322.1"
                     /translation="MKNLMILNDAALKKTLAKMHPYDIATKMKDASGDTQMRLIRLMA
                     LNKTVEVFLELPSEVSRHYFSLLTETQKKQFLEAFEMDDLKTFITSFEDDYQMYLLSL
                     LGTHKAIALKVLMSYDDEKIATIMSTNFVTITQNSTIKDTTAHIINSSKYNDFVDDIF
                     VINENNALIGSITLKDLISARATDTLDSITYSINNYLTLDDSLNDGYKKFENYAKSVL
                     PVLDDDMKIVGIVTADDVLNIMVAEHEEDIAKMTGVGDYDESSNAFRRSIQRMPWLVI
                     SVLLNLVIAALLSVFEKTLVEVIALIMFQPMILGMAGNIGTQSIAVTILKLNQDELAG
                     IHNEKKHIGKELAIGVLNSITIGLGGFLLSFLILSFINMGTHSPLLLAITIGTSLMGG
                     MFISAAAGVFIPIILEKMKVDPAIASGPIISTLNDLFALLIYFGIATLIFLL"
     gene            complement(5711..7015)
                     /locus_tag="PK460_04275"
     CDS             complement(5711..7015)
                     /locus_tag="PK460_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOE54323.1"
                     /translation="MRTIIKRKDKKALLKYVKKMHPYDIIEALNTLTLDEQELFYSLL
                     SEEEIGRIVAFMEPEKAAEVIDDFDLTKQKNIIDTLELDDAADIIVYLDNETKLIEGL
                     EKELHITQVLKYDEEKVGAHMSDAYISLSLGTDIKAATKNVIQKAGEIDVINHIFIVD
                     SGNKYKGTVELRDLVRTKAPALIDDITEFTPFVFASDDIEESVHKMKNYGLHEMAVVS
                     EELELIGIITIDDAIEIYHEESVDDFEKLSALPDTKTKGVIKSAAKRLPWLFLLLVLS
                     MPIAIATSRFEEVITSVAVLALFQPLILDAGGDVASQTLAVTLRLLSKNPKKALRNGG
                     TEILTGIINGLGLGIVAGIVSFIISKVIGITEPFNVSLVVFISLAVTVATGPIFGLFI
                     PLILDKMKIDPAVASSPLITTLIDITSVVIYFGVATLLLGVI"
     regulatory      complement(7090..7255)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
BASE COUNT         2341 a         1267 c         1108 g         2648 t
ORIGIN      
        1 attaagaata tccttacaat tataattgat tcaccccaaa cgactgttgg gtcccccact
       61 cttcggattc ggagtcccct atatcttatt agatatagta tggtaagtca attttttttg
      121 gtgcgtgact ataaaaaaat ccgcgttaag cggattctac tttctttacg actagttttc
      181 gatacgctaa atcaaataat gatacgaaca cagaagttaa aatcagtgtc acaattgtaa
      241 atataccaac ttgggtaaac tccacaccat taccatcaat aatcgttact gagataatca
      301 tacttataca tgctagaact aaataaaaag tttcggctaa taatttagcg acaaaaagat
      361 tatttgtttt tttattaatt agacgcatga tttcttcggg tggaccagca gggaattctg
      421 tgacaatcac aaggaaaaca ccaaaagaga taattaaaat cccaagtaaa gcaactagtg
      481 cacggatgaa aaatgtagta ttaagcgggt taaatgtatc aaaaagtaaa ccccaaatgg
      541 aaattacgag tgaaacaata aatatattta gccatgtaaa aattaaggct ttatttttag
      601 taataataaa taagacaagc actgttagtg tcccaaacgc taatgaagca acaccgattg
      661 ttgttttaag tttactaaaa tcactattat taaaaagttg taagtaaatt cttgctaagt
      721 aatagttcat cgcatcgatt ggtgctgttc caacatttgt ataaataatt atttggacac
      781 caaaaccaac taagataatg cctaaaagat atacaattag cttaataatg aatgttttgt
      841 taatttgaaa cttttgggtt ttattttcgt tttgcatact tcgtaattat aacaatttta
      901 tagtgctaat acttaaaata taaatgtaaa aatacatgag ttagttgtat ttattcattg
      961 taaattttat tagcgtaagt ccctactgct agaacaagta aaataaataa tttgattgtc
     1021 ttgtgataat ttttcaagta ggggtcttac gagtttaatt cttttatcat cgaagttaac
     1081 gaatgtatca tcaagaatga taaatggttt taagtcttga tacactaaat caattaaggc
     1141 aacgcgcact aagaccgaaa taagatcttg actaccagca ctgtaataag caaagtcgta
     1201 atattcattc gtgtctaaat cttttaattt catttcagag tttccgtcaa acattacaga
     1261 gtagccctct aatttaattt tcgtaagtaa gtcgttaaca ttattttgaa gcggggttaa
     1321 ataccttgct gctagttgct gattagcgtg ttctaataat gttctagcga tagttaataa
     1381 ttcttttttc tgctcatagt tttgcattgt agtgcgtaat tcgtcgcgtt caaacttgta
     1441 attttcaagg ttactcgcta agtcttcatt tgcttcaatt tgtttttcta aagctgttat
     1501 ttttttgatt aattcatcgt ttttgatgtc gacattactc ttattcctgt ttaattcttc
     1561 ttcatctagt agggcgaaag aatttaaatc actatttatt tttttaatat cataattctt
     1621 aagtttaaaa tttacatcat taagcttttt attaagcgct aaatgttttt catagtgact
     1681 attaataagg aaataagcaa cttgaacatc actaacacta ttaactttaa cggaatactt
     1741 acgaaataca tcaaaaacat gttctttaaa agcttctaat tctttttgtt tctggttaac
     1801 tttttgtgtc cactcatcaa gtttttgttt aagtttaatg tcactttctg tgctagtcat
     1861 tgtcgctagt tcagcggcat aattttcaag ttttacatta agttcatcaa tcgttttatt
     1921 taaaagcgct atttcttttc ttaacttatt attcttactt ttaaggacaa agaaataaat
     1981 tgatagcgtg ataatgctta ggaaaatatc aaggaaacga aatcttttta tatttgcatt
     2041 aagtaaagtt acttcatttt ctaatttagt aatttcttca ctagtttctt tatgcgctct
     2101 tttaataaat aagacttcaa catttgtact ttcgagagtg tttagagcag cgtttattga
     2161 attaatttct gtttgtgctt catctttaaa tttattaaat tcactctctt ttgccgacaa
     2221 atccgtgctt aattcttcta aatacactaa atttgtgtct tcaactttat tacccgcaaa
     2281 taatttttta ttttcttcaa tttgtaaatt aaggttattt atttcatcat ttagtgcatt
     2341 ataatcttta aggtcttttt caagtatctt tttattcgca acgagtttta aatcattttc
     2401 aagtttagtt tttagcttat cattttcggc tttttgtctt tttagcgcaa ctaattcatc
     2461 agagttttca ctaattaaat taacagcgtt ttctgcttca aacacaagat tatttgtggc
     2521 attgagttca cggtcagttt tgttatatag tgaatttctt cgctggttac caggtgaaag
     2581 ttcttttata gcatcatcaa gaatattaaa tgcggaagtg taatcactat cttctgttgt
     2641 atgcccaaac agaccgcgta attttacatt aatatcatca tgaatttttt catcactact
     2701 atataaaact tctaattgtt gaataaagtt taatttctca aaactttcac tatttacacc
     2761 aaagatgata tcaccgaggt tatcacgcgt tattttaata ccatttaatt catcaatatt
     2821 tacattgcgc gctaagtctt taactgcaat cgtatcatta gagagcgatt caccaaaggt
     2881 gcgggtaaca gtataagttc gcccgttgta gacaaaagtt aagttaccac cataggtggt
     2941 tttaccccac ggtaaatatt taaagcgtgg tagtgatagt ctttttcctt ttcctttagc
     3001 ggttttcatc ccaaagaaca tcgctttaat gaattgcgca atcgttgttt taccaaaact
     3061 attatcttca ttaatgattg taagcttttg atcaaattta agattgtaat tttcaagctt
     3121 accaaaacta tcaatattaa gttctaatat tctcataggt caattttcct tccctttagt
     3181 gcatttagac ccaattttaa gagttcttcc ttaacttcag gggagtaatt tgaagcttcg
     3241 attaggcgaa agaattcacc gcgtaatgat acttcgtttg caaaatcaat agtgttaagg
     3301 ttaaccttag aatcatcgat taactctgca taataaaatt tactttctaa atattcgcgc
     3361 agtgcattta tatctttgat taattcataa tcatatttac cagttaattt tagacgtacg
     3421 atatcatcac taggaatctt attagtggcc ttcttaattg ctaactcaat ttcaagagtg
     3481 ttgtttaatg cactgatatc gacatcaata atatgataag tacgactatt atttttaata
     3541 aattcgcgtc taatcagctt atttttttgt atttctaaaa caaaaaagcc gcgttcaccc
     3601 acttcatcaa acccgcgcgg ttcaagcaca cctgaataag catatatgcc gcgcgcatct
     3661 aatcttttcg cggcaatatc ggggatatgg atgtcgccaa gcgctaagta gtcaatattt
     3721 ttgcctttta aattatttaa agggatttca gcttcagtgc catgtagcat caagatgtta
     3781 tatttatttg gatcaaagat taaggtttta gtgtaatctt catctgcata gttatcataa
     3841 gctttaatac atacatcatc gtaaaggaat gtattatttt cttttgtcac aattttaaga
     3901 tttgaaggta attccgctaa tttttcatga ttaaatacag ctgggtcatc atgattaccc
     3961 gctaaatata taaaatcaat tgttggtgca cttgtaattt ttgttaatac ttcacgtaag
     4021 gtagcaagtt ttacttcttt attgtcaaat aagtctcctg cgattaagat aactttaaca
     4081 ttgtgcttaa ctccgtaatc gatcatgcga aaaaaagcat ttttaatttc tctaatgcgt
     4141 ctttctgttt tatcgccacc ataccgattc atctttgaat caatatgaat atccgctgta
     4201 tggattattt tcatacttac ccccgttaac ttacatttag ttaacttatt ttatcataat
     4261 acctagccat taaagtgaat atagcattaa taaatgctat tagccttcat taataaaaaa
     4321 agacatcatg tggcgatgtc ttttatattg tttgtaagca tctcttctct tgctcttata
     4381 gtaagaagat taatgttgca attccaaaat aaattagtaa tgcgaataag tcatttagcg
     4441 tcgagataat tggaccacta gcaatcgcag gatcaacctt catcttctct aaaataattg
     4501 ggataaaaac accagcggca gcagagataa acatcccgcc cataagcgaa gtcccaatcg
     4561 tgattgctag aagtaacggg ctatgcgttc ccatattaat gaaactaaga ataagaaatg
     4621 agagtaaaaa accaccaaga ccaatagtga tgctatttaa gactccgatt gctagttctt
     4681 tgccaatgtg ttttttctca ttatggatac ctgcaagttc atcttgattt aattttaaaa
     4741 tcgtcacggc aattgattga gtgccaatgt ttcctgccat ccctaaaatc attggttgaa
     4801 acataattaa ggcgatgact tcaacgagtg tcttttcaaa tactgataat agggcagcaa
     4861 tcactaagtt taataatact gaaataacta accacggcat tctttgtata gaacgtctaa
     4921 aggcattgga tgattcatca taatcaccaa cccctgtcat cttagcgatg tcttcttcat
     4981 gttcagcgac cataatattt aatacatcat cagcagttac aatcccgaca attttcatat
     5041 cgtcatctag tactggtaag acactttttg catagttttc aaacttttta taaccatcat
     5101 taagtgagtc atcaagtgtt aaataattgt taatgctata agttatgcta tcaagtgtat
     5161 cagtggcgcg tgcactaatt aaatctttta aagttatact tccaattaaa gcattatttt
     5221 cattaattac aaagatatca tcgacaaaat cattgtattt tgagctatta atgatatgcg
     5281 cagtagtatc tttgatcgtt gaattttgtg taatcgttac aaaattagtg ctcattattg
     5341 ttgcaatttt ttcgtcgtca taactcatta aaactttaag tgctatagct ttatgtgtcc
     5401 ctagtaaact taatagatac atttgatagt cgtcttcaaa actagtaatg aatgttttta
     5461 aatcatccat ttcaaaagct tctaagaatt gctttttttg tgtttccgtt aataaactaa
     5521 agtagtgacg cgaaacttca cttggtaatt caaggaacac ttcaactgtt ttatttagtg
     5581 ccatcaaacg aattaatcgc atttgcgtat cgccggaagc atccttcatt tttgtcgcga
     5641 tgtcataagg atgcattttc gctaaagttt tctttaaggc ggcatcattt aaaatcatta
     5701 agtttttcat ttaaatcacc cccagtaata atgttgctac cccgaaataa ataacaacac
     5761 tagtaatatc aataagtgta gtaataagtg ggctagaagc gactgctgga tcaattttca
     5821 tcttgtctaa aataagcggg atgaataatc caaatattgg tccagttgcg accgtcaccg
     5881 ctagtgaaat aaagacgact aatgatacat taaagggttc agtaatccca attactttgg
     5941 aaataataaa agagactata cccgcgacta taccaagccc aagcccgtta ataattcctg
     6001 ttagtatttc ggtgccacca tttctaagcg ctttttttgg atttttacta agtaatctta
     6061 aagttaccgc aagggtttga ctcgcaacat cacccccagc gtcaagaatt aatggttgga
     6121 aaagggcgag taccgctact gaagtaatta cttcttcaaa gcgacttgta gcaattgcaa
     6181 ttggcattga tagcactagt agtaagaaga gccacggtaa tcttttagca gcggacttaa
     6241 taactccttt tgtttttgta tctggtagtg ccgacagctt ttcaaaatca tcaacggatt
     6301 cttcatgata tatttcgatc gcatcatcaa tcgtaataat accgataagc tctaattctt
     6361 cgctgacaac tgccatttca tggagaccat aattcttcat tttatggact gattcttcaa
     6421 tgtcatcact ggcgaaaaca aacggagtaa attcagtgat atcatcaata agtgctggtg
     6481 ctttagtgcg gactaaatct cttaattcaa ccgtcccttt atatttattg ccactatcaa
     6541 caataaagat gtgattaata acatcaattt ccccagcttt ttgaataaca tttttagttg
     6601 ccgctttaat atctgttcct aaagaaaggc taatatatgc atcagacata tgggcgccaa
     6661 ctttttcttc gtcatacttt aatacttgcg taatatgaag ttctttttct aaaccttcaa
     6721 ttaacttagt ttcattatcg agatagacaa taatgtcagc agcgtcatca agttcaagcg
     6781 tatcaataat atttttttgt tttgttaaat caaaatcatc aataacttct gcagcttttt
     6841 ctggttccat aaaagcaaca attctgccaa tttcttcttc gcttagcagt gagtaaaata
     6901 gctcttgttc atcaagagta agtgtattta gtgcttcaat aatgtcataa ggatgcattt
     6961 ttttgacata tttaagcaat gcttttttgt cttttcgttt aataattgtg cgcatgttaa
     7021 cctccctcct ttgcttggaa ttcaaataaa aagctcccac attaagtgag agctaactaa
     7081 acagtacgag tatcagcgta ttcgtagagt tttagcacta tgcaacgtaa acatgaagtt
     7141 agctactttg tggaaagcct taagtcgaca atccatgttc tacccattgg tgtctctgga
     7201 catttttggg cagcagccta tatttgcata ggagcctcac ctaacggatt tatttaattc
     7261 caaagtccat aatagcatat atattatatt tgtcaatcta ctattttaaa aataatagaa
     7321 gaaaaggtta aattgacaag ataaaaaact gaaagaatca tttt
//
LOCUS       DAOSLP010000042         7095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_9633_length_7095_cov_7.652557, whole genome shotgun sequence.
ACCESSION   DAOSLP010000042 DAOSLP010000000
VERSION     DAOSLP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9633_length_7095_cov_7.652557"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK460_04280"
     CDS             <1..354
                     /locus_tag="PK460_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOE54324.1"
                     /translation="EKGIIKSYMYKSKTNEMYSGHKTKTGKFAETHYQVIKKGETYSL
                     VDINILTGRKNQIRVHLKDIDYPIVGDRKYGGDKSPINRLCLHAYELQVKHPLTSKTL
                     NFKSLTPKDFVELVK"
     gene            complement(399..1532)
                     /locus_tag="PK460_04285"
     CDS             complement(399..1532)
                     /locus_tag="PK460_04285"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE54325.1"
                     /translation="MLFSPEIKESPTLLLGEKARQKKEKGEKVISLAVGEPDFPTPEY
                     IIEATKKALDEGYTKYSTPQGLLELRTLIAKEYKPYKAEEVIIFPGAKAAIHAALTSI
                     LEPGDEVIIVEPYYVSYPAMIKLAEPKAKIVKIALNKDFTFPLEKLEEKITKKTKLLI
                     LNYPHNPTGQILSFQETMKIVNLVFRNKIYLLSDEIYDKLNFSGKAFHSFAEFPLIKD
                     QLVVINGFSKTYAMTGFRLGYALANKELINKMNLVNQNINTNTATFIQKGAVAALTNK
                     PMFLIPYNNELKERALYLHEEINKLPLFSGVLPEGAFYYFMNISKTKLTSTEFANKLL
                     EEHNLLVVPGIAFGEKFDKYVRLSLCVSMDTLKETVEILKEFK"
     gene            1636..3342
                     /locus_tag="PK460_04290"
     CDS             1636..3342
                     /locus_tag="PK460_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOE54326.1"
                     /translation="MKTFHFNDDYALINFDGINVNDALLILNSKSFEKVFRSFHRSLK
                     EKESVLLVSLGKLTATDLLEFYSLLFKYELSTVLRRMPKVTKDHVLPIYELTESFYDY
                     WRKYERFGLLNAFKEDGTQTKVDDLLKVSNDFNYQIITIYRTISQKLLGQTFNVYRQL
                     PAGISANMLYTYHSFDKSLKEYKLLNNIPVATKIVINPPFIINSKSNVRKGVFTPLTY
                     SYNPITHLKINKNNFIAFPLWVGPLLAYVYIHIDLLHHGIALSNLFEIADYQKVKDLK
                     PNLIYIYGVEDSLYDSKFYHDTVNDIYLGFVYKSAENDYFGYLKKMLLTLHNLYMINH
                     DNLPVHGAMVEITLKSNIKKRVVIVGDSGAGKSETLEALRQIASDEIKDIKVVFDDMG
                     TLSTNEDGKVLASGTETGAFVRLDDLEQGYAYEVMDRALFLNPSQINSRVVIPLSRYS
                     FILEKYPIDLFLYANNYEDKDGIKVFDKPEEALAVFRKGMRRAKGTTSEVGMTASYFA
                     NPFGPMQYRAKTDRLLNKYFKALSDNKVVLGELYTKLALPEEGVRGPANAALALFKLL
                     QG"
     gene            complement(3414..4931)
                     /locus_tag="PK460_04295"
     CDS             complement(3414..4931)
                     /locus_tag="PK460_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOE54327.1"
                     /translation="MRRLATIGLLVILALTGCGKRTSSEVSLTTSAPSLSDEPSIATP
                     LSIQVFKNDIRAYDVTVDVSHTINLEARVLPLDAPQEVVWSVVNSELATVSATGAVTG
                     VAPGETMINVHALNYPDVKTSLFLTVNKVAEQTGVGSGTSADDPLFKGYEGDGPLEVY
                     FLETYKIYADSLYIKKGNIDIVIDGGWKCDGELNRDFLNEKVTDGRIDMLIASHGDGD
                     HTQGIPYLVRDIPHISSFLDYGRAGGESSGYKQLIRTFGENEGSPYYTALDSVNKVNG
                     ATNRYYFTSEFYVDVLNTGAYSDTSSGNANSLALMFCYKDFKFFTAGDLTTGSETSLL
                     RNETLPQVSLYKASHHGSHGSNSQALMDTLNPYTVAIAATRAGIYNQDPAPPSPTNTY
                     NLDGTKGHPAAAAIERIYKIPRISLNLNVFWNAPNGTMKFTTYGGANDLVMNGSTPLR
                     HYYDLTLTNGEPVWNEELQDFENKVTGEENTKFHETKIFTFRGYTEYLPAWVTAA"
     gene            complement(4936..5268)
                     /locus_tag="PK460_04300"
     CDS             complement(4936..5268)
                     /locus_tag="PK460_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54328.1"
                     /translation="MYAIRTQSNNDQARSGRIELQDNVYDFNMLEFDAAIYDANRKGM
                     VLMFEVSTDDGVTWTQLEREYTINHFDLVKYRVRTGTTGNVRVAIVLKAGTGQRINID
                     NIRLIKEA"
     gene            complement(5317..7035)
                     /locus_tag="PK460_04305"
     CDS             complement(5317..7035)
                     /locus_tag="PK460_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOE54329.1"
                     /translation="MKRRKLLLIPLIALLLGGCGDSSSSAVVTSEAPVTSVSEEVTPT
                     GITISGPGSLAVDESAYFNVTVKPEGAPQSVTWSTSDATKATVTESGEVTGVSEGNVF
                     VIATSTVKAEVTAQVPLVITPKVIVAPDPTEILITSVDDLTTVSVDKTLKLNATVLPV
                     DASQEVNWSSSDETKATVDATGLVTGVALGTVNITATSAVKESVKKVFTLEVVEDAIT
                     QNWEEMAYSSHADFVNKNTEDGTPFKVRGVVTYAAPVTAEGTVEYFIQNGTEGYYIYK
                     QDNDLFPVTVGNSYTVGGYFKNHFGTFELVDVEYFVPHDPALTYEVVDISDKDVLSLD
                     IMAEHQASFVEIKAAVVSVLPTNYTKAYNVKVNVGDKEFTLRVDPALMSADEFNAITA
                     LFQGTAPGSAIDVVGVMSAFGYGTPANQMKIVRSTDITAAGMTDKQATEVAAASLEIV
                     ESIPLNVNNVTLPTTLEGYEGVTVEWTSSNAALNTETGAVTHPVSTLDLTLTATVKKG
                     TDQTVRSWTVTVFGTNLESLSFLHTLDLEDADPSGSYGCSNTKPKYTVEATNDMVDLG
                     DPVTKW"
BASE COUNT         2182 a         1320 c         1282 g         2311 t
ORIGIN      
        1 gaaaaaggta tcattaaatc ctatatgtat aaatcaaaaa caaatgaaat gtatagtggc
       61 cataaaacta aaacaggtaa gtttgccgag actcactacc aagttattaa aaaaggtgaa
      121 acttactctt tagtagacat aaacatccta acaggtcgta agaatcaaat tagggttcac
      181 ctaaaagata ttgactatcc tatagtgggt gatagaaaat atggtggtga taaatcgccg
      241 attaatagat tatgtttaca tgcttatgaa ttacaagtaa agcatcctct tacaagtaaa
      301 acacttaatt ttaaatcttt gactcctaaa gattttgtag aattagttaa atgaaaagtg
      361 gacgagccga ttacttgtcc gtttattaat aattgatttt atttaaactc tttcaatatt
      421 tccactgttt cttttagtgt gtccatactg acacataaac taagtcttac atatttatca
      481 aacttttcac caaaggcaat acccggcact acaagaagat tatgttcttc taataattta
      541 ttagcgaact ctgtacttgt tagttttgtt ttactaatat tcataaagta atagaaagca
      601 ccttcaggta atacaccact aaagagtggt aatttattta tttcttcgtg taaataaagt
      661 gctctttctt ttagttcgtt attatacgga attaaaaaca ttggtttatt agttagtgcc
      721 gctactgcgc ctttttgaat aaaggttgcg gtatttgtgt taatattttg atttactaaa
      781 ttcatcttat ttattaattc cttattagcg agagcatatc ctaatctaaa ccctgtcata
      841 gcgtatgttt tactaaagcc attgatgacc actagttggt ctttaataag cgggaattct
      901 gcaaaagagt ggaaggcttt accgctgaaa ttaagtttgt catatatttc gtcacttagt
      961 aaatatatct tattcctaaa tactaaatta acaattttca ttgtttcttg gaatgataag
     1021 atttgaccag tggggttatg cggataatta agaattaata attttgtttt tttagtaatc
     1081 ttttcttcta acttttctag tgggaaagta aaatctttat taagtgctat tttaacgatc
     1141 ttagctttag gttccgctag tttaatcatt gcgggataac taacgtaata aggttcaacg
     1201 ataataactt cgtcacctgg ttcaagaata ctcgttaatg ccgcgtgaat tgccgcttta
     1261 gcgccgggaa agataattac ttcttcagct ttgtaaggtt tatattcttt agcaattaaa
     1321 gttcgtaatt ctaataaccc ttgcggagtg gaatatttcg tatatccttc atcaagtgct
     1381 ttctttgttg cctcaattat atattcaggc gtaggaaaat caggttcgcc taccgctagt
     1441 gaaataactt tttcaccttt ttcttttttt tgccgcgctt tttcaccaag taaaagtgtc
     1501 ggactttctt ttatttccgg actaaataac ataaaaaata cctcttttct tatcaatttt
     1561 accatatttt gctcattttt tgataaaaaa tggtcgctta tggtacatta tataattaag
     1621 agaaggggaa tattgatgaa gacttttcac tttaatgatg actatgcact aattaacttt
     1681 gatggtatta atgttaatga tgcgctctta atattaaact ccaaaagttt tgaaaaagta
     1741 tttagaagct ttcaccgctc tttaaaagaa aaagagagtg ttttacttgt tagtttaggt
     1801 aaactaaccg cgactgattt attagagttc tatagccttc tttttaaata tgaacttagt
     1861 actgtgttaa gacggatgcc taaagtcacg aaagatcatg ttttaccaat atatgaatta
     1921 accgaaagct tttatgacta ctggcggaag tatgaaagat ttgggttact taatgctttt
     1981 aaggaagatg gtactcaaac taaagtcgat gatctcttaa aagtaagtaa tgactttaac
     2041 tatcaaatta ttacgatata caggactatt agtcaaaagt tattaggaca aacgtttaat
     2101 gtatatcgtc agttacccgc tggtataagc gctaatatgt tatacacata tcactctttt
     2161 gataaaagtt taaaagaata taaactgctt aataatatcc ctgtagcgac gaaaatcgtt
     2221 attaacccac cctttatcat taattcaaag agtaatgtga gaaaaggagt ctttacacct
     2281 ttaacttatt cttataatcc aattactcac ttaaaaataa ataaaaataa ctttattgct
     2341 ttcccgttat gggtgggacc acttctagcg tacgtctaca tccatattga tttattacac
     2401 cacggtatcg ctttatctaa tctttttgaa atcgcggatt atcaaaaagt caaagactta
     2461 aaaccaaact taatttatat ttatggtgtc gaagattcgt tatatgactc taaattttat
     2521 cacgatacag taaacgacat ttatctcggt tttgtctata aaagtgctga aaatgattat
     2581 tttggttatc ttaaaaagat gttacttaca ttacataatc tttatatgat taatcatgat
     2641 aatttaccag tacatggtgc aatggtagag attactttaa aatcaaacat taaaaaaaga
     2701 gtagttattg ttggtgatag tggtgctggg aaaagtgaaa ctttagaagc attacggcaa
     2761 attgcaagtg acgaaattaa agacattaaa gtcgtgtttg atgatatggg gacactaagc
     2821 actaacgaag atggtaaagt acttgcaagt gggacagaaa caggtgcttt tgttagacta
     2881 gatgacttag aacaaggcta tgcttatgaa gtaatggatc gagcactatt tcttaatcct
     2941 tcgcaaatta actctagagt ggtaattcct ttaagtagat actcatttat tttagaaaag
     3001 tatccgattg atctcttctt atatgcgaat aattatgaag ataaagacgg gattaaagta
     3061 tttgataaac ctgaagaagc actcgctgtt tttcgtaaag gaatgcgaag ggcaaaaggc
     3121 actacgagtg aagtcggaat gactgcttct tacttcgcta atccgtttgg tccaatgcaa
     3181 tatcgcgcta agacagatcg attacttaat aaatacttta aagcgcttag tgacaataaa
     3241 gtagttttag gggaactcta cactaaatta gcgcttcctg aagaaggagt taggggaccc
     3301 gcgaacgctg cgctagcgct atttaaatta ctacaagggt agtaagatcg ttttaactat
     3361 tcataaagaa aagtcaggaa tcgctcctga cttttttgct gtattttaat tatttaagca
     3421 gcggtaaccc acgctggtaa gtactcggta taacctctaa atgtaaagat tttagtttca
     3481 tggaacttag tgttttcttc acctgttact ttattttcaa agtcttggag ttcttcgttc
     3541 catacaggtt caccgtttgt gagtgttaag tcataataat ggcgaagtgg ggtactacca
     3601 ttcatcacta agtcattagc gccaccatat gttgtaaatt tcattgttcc gttaggagca
     3661 ttccaaaaga catttaagtt taaagaaatg cgcggaattt tataaatgcg ttcaattgct
     3721 gctgctgcgg ggtgaccttt agtgccatct aaattatatg tattagtagg acttggtgga
     3781 gcaggatctt gattgtatat accggctctt gtcgcggcaa tcgcaacagt ataggggttt
     3841 aatgtatcca ttaaggcttg actgttacta ccatgactcc cgtggtgact agctttgtat
     3901 aaacttactt gtggtaatgt ttcattgcgt aaaagactag tttcactacc agtggttaag
     3961 tcaccagcag taaagaattt aaagtcttta taacagaaca ttagtgcgag actattagcg
     4021 ttaccgctgc tagtgtcact atatgctccg gtatttaaaa catcaacata aaattcactg
     4081 gtgaagtaat agcgattagt cgcaccgttt actttattca cggaatctag agcagtgtag
     4141 tatggcgaac cttcgttttc accaaaagtg cggattaact gtttatagcc actgctttcg
     4201 ccgccagctc tgccgtaatc aaggaagcta ctaatatgcg gtatgtcgcg cacaagataa
     4261 ggaatacctt gtgtatggtc accgtcccca tgactagcaa taagcatatc aatccttccg
     4321 tcagttacct tttcatttaa gaagtcacga ttaagttcac cgtcacactt ccagccacca
     4381 tcaatcacaa tatcaatgtt gcctttttta atataaagcg aatcagcgta tattttatat
     4441 gtttcaagga aataaacttc aagtgggccg tcgccttcat agcccttaaa aagtggatca
     4501 tcagcgcttg taccacttcc gacacctgtt tgctctgcaa ctttattaac agttaaaaac
     4561 aaactagttt taacatcagg ataattaagc gcgtgcacat taatcattgt ttcgccaggc
     4621 gctacaccag ttaccgcacc tgtagcgctc acggttgcta gttcactatt aacgacactc
     4681 cacacaactt cttgcggggc atctagcggt aatactctag cctccaaatt aatcgtgtga
     4741 ctaacatcaa ctgtgacatc atatgctcta atatcatttt taaaaacttg aatgcttaaa
     4801 ggtgtggcta ttgaaggctc atcacttaat gaaggtgcac ttgttgtgag tgacacctcc
     4861 gaagaggtgc gcttaccgca cccggtaagc gctaagatta ctaataaacc aattgtggct
     4921 aaacgtctca taagcttaag cctctttgat taagcgaata ttatcaatgt tgatgcgttg
     4981 accagtacca gcttttaaga cgatggcaac acgaacatta cctgttgtac cagtacgtac
     5041 acgatactta actaaatcaa agtgattgat tgtgtattcg cgttcaagct gcgtccaagt
     5101 gacaccatca tccgttgaaa cttcaaacat caagaccatg cctttacgat tagcatcata
     5161 aattgcagcg tcaaattcaa gcatattgaa gtcatagaca ttatcttgta actcaatccg
     5221 tccagaacga gcttggtcat tattactttg ggtacgaata gcgtacattc cagttcgctt
     5281 atcattatta tcgccgccaa ttaaggcgtt tttaagttac catttagtaa cagggtcgcc
     5341 taaatcaacc atgtcatttg tggcttctac agtatatttt ggttttgtat tactacaacc
     5401 ataactaccg ctaggatcag cgtcttctaa gtctaatgta tgtaagaatg aaaggctttc
     5461 taagtttgta ccaaagacgg ttacagtcca agagcgaact gtctggtcag ttcctttttt
     5521 gactgttgcc gttaatgtga ggtcaagtgt tgaaactgga tgcgtaacag cacctgtttc
     5581 cgtgttaagt gctgcgttac ttgaagtcca ttcaacagtt acaccttcgt agccttctaa
     5641 agtagtaggt agtgtaacat tattaacatt aagcgggatg ctctcaacga tttctagact
     5701 tgcggccgcg acttctgtgg cttgcttatc agtcatccct gctgccgtaa tatcagtgct
     5761 tcgtacaatc ttcatttggt tagcgggagt gccatagcca aacgcactca taacaccaac
     5821 aacatcaatc gctgaaccag gagcggtgcc ttggaacaaa gccgtaatcg cattaaattc
     5881 gtcagcactc attaacgccg gatcaacacg aagtgtaaat tctttatcac caacattaac
     5941 tttgacatta tatgctttag tatagtttgt ggggagcaca ctaacaacag cagctttaat
     6001 ttctacaaat gaagcttggt gttccgccat aatgtctaaa cttaagacat ctttatcact
     6061 aatatccaca acttcataag ttaaagctgg gtcatgtggc acaaagtatt caacatcaac
     6121 gagttcaaat gtgccaaagt ggtttttaaa ataaccaccg acggtgtaag aattaccaac
     6181 agtaacaggg aataagtcat tatcttgttt gtagatatag tatccttcag taccattttg
     6241 gataaagtat tcaacagtac cttcggctgt tactggagcg gcgtaagtga caacaccacg
     6301 gactttaaaa ggagtgccgt cttccgtatt tttattgacg aaatcagcgt gtgagctata
     6361 cgccatttct tcccagtttt gggtaattgc gtcttcgacg acttcaagcg taaagacttt
     6421 tttaacactt tctttaacag cacttgttgc cgtaatattt actgtgccaa gcgccacacc
     6481 agtgactaaa ccagtggcat ccacggttgc ttttgtttcg tcactactag accaattaac
     6541 ttcttggcta gcgtcaaccg gtaaaacggt ggcatttaat ttaagtgttt tatcaacact
     6601 gacagttgtt aagtcatcaa ctgaagtaat aagaatttca gttggatcag gagcgacgat
     6661 tacttttggc gtaatcacaa gtggtacttg ggcagtaact tcggctttaa cagtggaggt
     6721 agcaatgaca aagacattac cttcactaac accagttact tctccgcttt cagtgactgt
     6781 tgctttagtg gcatcgcttg tcgaccaagt gacactttgc ggtgcgcctt ctggtttaac
     6841 agtaacatta aagtaagcag attcatcaac cgctaacgag cccggcccac tgattgtaat
     6901 accagtagga gttacttctt cactaacact agttacgggc gcttcactag tgacgactgc
     6961 actactgcta ctatcaccac aaccacctaa aagtaaggca ataagtggga tcaataataa
     7021 ttttcttcgt ttcataaaca tcttgtatta tctaatcatg ccccggtact tcgattaggt
     7081 aatacatctc ctttc
//
LOCUS       DAOSLP010000043         7092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_9636_length_7092_cov_7.978116, whole genome shotgun sequence.
ACCESSION   DAOSLP010000043 DAOSLP010000000
VERSION     DAOSLP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9636_length_7092_cov_7.978116"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..903
                     /locus_tag="PK460_04310"
     CDS             19..903
                     /locus_tag="PK460_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE54330.1"
                     /translation="MFKSKFAIDPSLLHKDSVYTLNNGVKMPVIGFGTWQAKDGEEAY
                     ESVKAALRVGYRHIDTAEAYHNEESVGRAIRDSGIPREEIFVTTKLTNTHLTYEDAKQ
                     AIDASLKRLGLDYVDQYLIHWPNPIDARTFYKKRNAEVYRAMEEAYFAGKIRSIGVSN
                     FHPHHLKALLKTIKVKPATNQIYVSPSDTQPKVVAFNEKHNILTVAYSPLGTGRLLGL
                     NELNQVGTKYNKRPAQVAIRWSLEHGYLPLPKSTNASRISENFDVFDFKLSKEDIKLI
                     NSLKGKAGKAPDPDHVIW"
     gene            complement(910..3402)
                     /locus_tag="PK460_04315"
     CDS             complement(910..3402)
                     /locus_tag="PK460_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE54331.1"
                     /translation="MKKRLLSLFVISVLFSNVPSTSTKIVETNAQTGITVHYRGDYTN
                     PHIYYWNPQPAIITTPSWPGEKMIKDEGSDWYTKSWPELTAINLIFNNGGQPQTSDLS
                     RTTGEYWWVGQGTGGKWYTSDPDTITEPEYTDTGKLVIHVKSPHSVPKIKYYDSVPSG
                     ISTPNAINMTAEKEGWYRFGFNKISSIKAKFTIDGSESAEYFFTNGEWYLENGVLTDF
                     KPKVTSGKSDFREETIYFLMTTRFYDGDTSNNVHCWDDAKAGNLASDPAWRGDFKGLI
                     QKLDYIKALGFSAIWITPIVENASGYDYHGYHALDFTQVDPRLESPNATYQTLINETH
                     ARGMKIIQDVVFNHSGNFGEKNIFPLFKKDYTKADTVSNLIKTDPHNLLGSDYDALDG
                     GRQYSRRINAMKEDANDFRHIYHHEKSLGWEDYNVQTGQIAGDCVDLNTENPYVSDYL
                     INAYNRYIDMGVDAFRVDTVKHISRLTFNNVFNPAFQNRGGDDFFMFGEVATRYRQVW
                     NNGNPAISTPFYTWKESKSYPWATREEREASTYQHWQDNANPATQPSSNNHLLDGNNY
                     RQVDYSKKSYMNVIDFPMHWNFKNANDAFNVAVGNDYTYADATWNVMYVDSHDYAPDG
                     APEGQRFAEPQSVWAENLSLMFTFRGIPTIYYGTEIEFQKGKPIDVGPNAPLSETGRA
                     YFGNHIEGSLTVTDFGKYTNANGAVATTLNHPLAKHIRNLNLIRRNIPALQKGQYSTA
                     GINGGLAFKRRFTDSTTDSFALVTISSSATFNNIPNGTYVDAVTGETKVVTNGSLSVN
                     VSGKGNLRVFVLTTSKTPAPGKIAESGLTYIK"
     gene            3553..6912
                     /locus_tag="PK460_04320"
     CDS             3553..6912
                     /locus_tag="PK460_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOE54332.1"
                     /translation="MKQRHKITILTLLALPLILGFTKWEKREKDPFITARAMPFVYPS
                     LNGEPKFSSQFHFNHINRGTVLNNYRGAGVTVAIIDSGLNTTHEDFLDGATTNISNKS
                     AYVEETGTYYSNIQIQSVEEHGRSIIEDTHPEAAGHGTNVASTVGAMVNGVGTAGISA
                     NVTLLVLKTNYMFTEINAAIRYAADNGADIINMSLGAYEESFIDGHGKQQTGIEGAST
                     YFQTAINYAYNKGVTVIAAAGNEKTDHYSYPASNNNVIGVGALGRNTSTTIASYSNFG
                     LHNVDIVSPGSVYVADIGGVSAYTETQGTSFAAPIVASAAAIYKGKYPTATPAKIEQR
                     LKETAYDLGAAGPDATFGYGRLDLSALLVDEVPVTGVEINPTSLTLRVGDTSQVSATI
                     LPENASNKNHIFISENDAIATVNEDTGVVTAHSVGTTRIGVLTDDLFEAYTDVTVVAA
                     GTPLEITGLDIVDTTINLGVGATKTLDINLTPNNANYNDLNFVSNNESVATISSAGVV
                     TALKSGIATITASAKVGTKSDTVTINVLNVTNATETYYFKDQYWGATPANWTSVKDAF
                     GYDTNRVQVTEGASGASATSPKSFTNIQGITFGVSSNASKGAGTVKVYLKETATSTTK
                     TEVGSFTISSTGGTTRRESPTIVPSINNSGFIQFEVICSTNSVYIHDIKIDYQEVVPD
                     PVAVTGVTINPKTLSLTVGENSQATFTIAPSNATNKEVTFSSLNTSVATVTSDGLITA
                     VSEGSTTIRVTTTDGNKTDDLSLTVSPAPVYEPYLNVDFSAFKKNYIFGEALALSSIM
                     AEHRASNGTITNLTGTDLTLVSGSTKELGVVPLNFSYDGLTASVNITVTNVGSYTGAV
                     AKDPLTIKGDSFAPAISTTTGTNYSSLSASCYTFAGVGLRNLDSYIMVYTSGHFRNVS
                     ALETIKSLKLDYKTGGSSASVQAFRFGTTEITSASGAAHTTLTSSAGGTSATVNAPAG
                     TTHFRIDVSSKNLQANITITFESGSTYTFTAAEQAAAYEDYFMAVTHEECLVTDVKLT
                     TWNHLKTEFDAMLPEAQNLIRTTASVEFNARYNIIVAGYGYTNFLVSGGTGLGRDLKN
                     FENKLPILIMGLGLPLSVCFYIALKKRKKTL"
BASE COUNT         2252 a         1273 c         1362 g         2205 t
ORIGIN      
        1 atttatagga ggggtcagat gtttaaatca aaatttgcaa ttgatcctag ccttttacac
       61 aaggatagcg tttatacact aaataatgga gtgaagatgc ccgtcatcgg ttttggcacc
      121 tggcaagcaa aagacggcga agaagcttat gaaagcgtaa aagcagcgct gcgtgttggt
      181 tatcgccata ttgacactgc cgaagcttat cataacgaag aaagtgttgg gcgcgctatt
      241 cgtgatagcg gtatcccgcg cgaagaaata tttgttacca ctaaattaac aaatactcat
      301 ttaacttatg aagacgcaaa gcaagcgatt gatgcaagtt taaagcgctt agggcttgat
      361 tatgttgacc aatatttaat tcactggcca aatccaattg atgctcgtac tttttataaa
      421 aagagaaatg cagaagtata tagagcgatg gaagaagctt attttgctgg gaaaattcgc
      481 tcaattggtg ttagtaactt tcatccccat cacttaaaag cattacttaa aacaattaaa
      541 gtaaaacccg ctacaaatca aatatatgta agtccaagtg atacacaacc taaagttgtc
      601 gcctttaatg agaagcataa tattttgaca gtagcctatt ctccacttgg cactggtcga
      661 ctattagggt taaatgaact taatcaagtt ggaacaaaat acaataagcg tcccgctcaa
      721 gtagcgatca gatggagttt agaacatggt tatttgccgt tacctaaatc cacaaacgca
      781 tcacgcatta gtgaaaactt tgatgttttt gatttcaagt taagtaaaga agatattaaa
      841 ttaattaatt cattaaaagg aaaagcgggc aaagcccctg atccagatca tgtaatttgg
      901 taaatataat tatttaatat aagttaatcc gctttctgct attttgcctg gtgcaggtgt
      961 tttagaagta gttaaaacga acactcttaa attgccttta ccagatacat tcacggataa
     1021 agaaccatta gtgacaactt ttgtttcacc agtaacagca tcaacataag taccatttgg
     1081 aatattatta aatgtagcac tacttgaaat tgtgacaagt gcgaagctgt cagtggtgct
     1141 atcagtaaat cggcgtttaa acgctaaacc gccattaata ccagcagtac tatattgtcc
     1201 cttttgtaaa gcggggatgt ttcttctaat taagtttaag ttacgaatat gtttcgctaa
     1261 tgggtgattt aatgttgttg caactgctcc atttgcgttc gtatacttac caaaatcagt
     1321 cacagtgagt gagccttcaa tatgattacc aaaatatgcg cgacctgttt cactaagtgg
     1381 ggcatttggt ccaacatcaa ttggttttcc tttttggaat tcaatttctg ttccgtaata
     1441 aatcgttggg atacctctaa aagtaaacat cagacttaaa ttttccgccc atacactttg
     1501 tggttcggca aagcgttgtc cttcaggagc gccatctggt gcatagtcat gcgaatcaac
     1561 atacattaca ttccatgttg catcagcata agtataatca ttcccgactg cgacattaaa
     1621 ggcgtcatta gcgttcttaa agttccagtg catcgggaaa tcaataacat tcatgtaact
     1681 tttctttgag taatcaactt gtcgataatt attaccatct agcagatggt tgttactgct
     1741 aggttgtgtt gctggattag cgttatcttg ccagtgctga tatgtacttg cttcccgctc
     1801 ttctcgtgtt gcccacggat aacttttact ttctttccag gtgtagaacg gtgtgctaat
     1861 tgcggggtta ccattattcc atacttggcg ataacgagtg gcgacttccc caaacataaa
     1921 gaagtcatca ccccctctat tttgaaaagc aggattaaaa acattattaa aggttaagcg
     1981 tgaaatatgt ttaacagtat caactctaaa agcatcaact cccatatcaa tatagcgatt
     2041 ataagcattg attaagtagt cactaacata aggattttca gtgtttaaat caacgcagtc
     2101 tccagcaatt tgtccggttt gcacattata atcttcccag ccaagacttt tttcatggtg
     2161 ataaatatgg cggaagtcat tagcgtcttc cttcattgca ttaatacgcc tactatattg
     2221 tctaccacca tcaagtgcgt cgtagtcact accaagtaaa ttatgcgggt cagttttaat
     2281 taagttacta acggtgtcag cttttgtgta atctttttta aatagtggga agatgttttt
     2341 ctctccaaag ttcccgctgt ggttaaagac aacatcttgg atgattttca tcccgcgtgc
     2401 atgtgtttca ttaattaaag tttgataagt tgcgtttgga ctttctaagc gcggatcaac
     2461 ttgagtaaag tcaagagcgt gataaccgtg ataatcatag cccgaagcat tttcgacaat
     2521 tggtgtaatc caaattgcac taaaaccaag ggctttaatg taatcaagtt tttgaattaa
     2581 gcctttaaag tctccgcgcc acgctggatc actagctaaa ttccctgctt tagcatcatc
     2641 ccaacagtga acattattag atgtgtcacc atcataaaat cttgttgtca tcaagaagta
     2701 aattgtttct tctctaaaat cacttttacc acttgttact tttggtttaa agtcagtgag
     2761 tactccgttt tctaaatacc attcgccatt agtgaaaaag tactcagcac tttcactacc
     2821 atcaattgtg aatttagctt taatacttga aatcttatta aagccaaagc ggtaccatcc
     2881 ctctttttca gcagtcatat ttatggcatt tggtgtactt attccgcttg ggacactgtc
     2941 ataatactta attttaggta cactgtgtgg tgacttaaca tggattacta atttaccagt
     3001 atcagtgtat tctggttcgg taatggtatc ggggtcactt gtgtaccatt taccacccgt
     3061 gccttgcccg acccaccagt attcgccagt agtgcgtgat aaatcactag tttgtggctg
     3121 tccaccatta ttaaatatta agttaatagc agttaattca ggccacgatt tagtgtacca
     3181 atcacttcct tcatctttta tcatcttttc accaggccaa cttggtgtag taataatagc
     3241 gggctgcgga ttccaataat aaatgtgcgg attagtgtag tcaccgcgat aatgaacagt
     3301 aatccctgtt tgtgcatttg tttccactat tttagtggaa gtacttggca cattactaaa
     3361 aaggacacta ataacaaaca aagataacaa tcgttttttc ataagtgttt ctccaacgct
     3421 aacttcatta taacaaagga tgacattatt attttggtaa gttgaaaata atgttttgtt
     3481 acacaaaaaa cttattattt atatattttg aatagtttca tgttattatc ttaattactg
     3541 gagattttta ctatgaaaca acgtcacaaa ataacaatcc taactttgtt agccttacca
     3601 cttattcttg gttttactaa atgggaaaaa agagaaaaag atccgtttat tacagcacgg
     3661 gctatgcctt ttgtgtatcc gtctttaaat ggtgaaccaa aattttctag tcaattccat
     3721 tttaatcata ttaatcgcgg cactgtactt aataactatc gtggtgcagg agtcacagta
     3781 gcaattattg actcaggact aaatacaaca catgaagatt ttttagatgg cgctactacg
     3841 aatatttcta ataaatcagc gtatgttgaa gaaacaggaa cttattattc aaatatacaa
     3901 attcaatctg tagaagagca tggacgaagt atcattgaag atactcatcc cgaagctgcg
     3961 gggcacggca cgaatgtcgc tagtacagtt ggtgcgatgg ttaatggtgt tggaacagcg
     4021 ggtatttcag caaatgtaac actcttggtg ttaaagacaa attatatgtt tactgaaatt
     4081 aatgcggcaa ttcgttacgc tgctgataat ggtgccgata ttattaatat gtcactaggc
     4141 gcatatgaag aatcattcat tgatggccac gggaaacaac aaactgggat cgaaggtgct
     4201 tctacctact tccaaacagc gattaattat gcttacaata aaggtgtaac agtaattgcg
     4261 gcagcgggaa atgaaaagac tgatcattat tcttatcctg cgagtaataa taatgttatt
     4321 ggtgttggtg cacttggtcg taatacatca acaaccatcg cttcttattc aaactttggc
     4381 ttacataatg ttgatatagt tagtccagga agtgtttacg tcgctgatat tggtggcgtt
     4441 agtgcttaca cggaaacaca aggcacatca tttgcagccc caattgtggc aagtgcagcg
     4501 gctatttaca agggaaaata tccgactgcg actcctgcta aaattgaaca aagattaaaa
     4561 gaaacggcct atgacctagg tgcagcggga ccagatgcta cttttggtta cggtcgtctt
     4621 gatttaagtg ccttacttgt tgatgaagta ccagttactg gtgttgaaat taatccaaca
     4681 agtttaacac ttagagttgg tgacactagt caggtaagtg caacaattct tcctgaaaac
     4741 gctagtaata aaaaccatat ctttattagt gaaaatgatg ctattgcaac cgttaacgaa
     4801 gatactggag tagtaaccgc tcatagtgta ggaacaacaa gaattggtgt acttactgat
     4861 gacttattcg aagcttacac tgatgttact gttgttgctg ctgggacacc gctagaaata
     4921 acaggtctag atattgtcga tacaacaatt aatttaggag ttggtgcgac taaaacacta
     4981 gatattaatt taactccaaa caatgcaaat tataatgact taaattttgt tagtaataat
     5041 gaaagcgttg caactataag tagtgctggt gttgtcaccg cccttaaaag cggaatcgct
     5101 acgattaccg ctagtgctaa agttggaaca aagagtgata ctgttactat taatgtttta
     5161 aatgtaacca atgcgacaga aacttattat tttaaagatc aatattgggg cgctactccc
     5221 gctaattgga caagtgttaa agatgctttt ggttatgata caaatcgtgt acaagtaact
     5281 gaaggcgcta gtggagcaag cgcgacatca ccaaaaagct ttacaaatat tcagggtatt
     5341 acttttggag tgtcgtctaa cgctagcaaa ggcgcaggta ctgttaaagt gtatttaaaa
     5401 gaaacagcta cttccactac aaaaacagaa gtaggtagtt ttacgataag tagtactggc
     5461 ggaacaacta ggcgtgaaag tccaactatt gtcccaagta taaataatag cggttttatt
     5521 caattcgaag tgatttgttc gacaaatagc gtgtatattc atgatattaa gattgattat
     5581 caagaagtag tacctgatcc agtcgctgtc actggtgtga cgattaatcc aaaaacattg
     5641 tcattaactg ttggcgaaaa tagtcaagca acatttacga ttgcaccaag taatgctacg
     5701 aataaagaag taacattttc aagcttaaac actagtgttg ctactgttac gagtgatggt
     5761 ctaataactg ctgtaagcga agggagtacg acaattcgcg ttactactac agatggtaat
     5821 aagactgatg acttaagttt aacagtttca ccagcaccag tttatgaacc atatttaaat
     5881 gttgatttta gtgccttcaa aaagaattat attttcggtg aagctttagc gcttagttca
     5941 attatggcag aacatcgcgc aagtaatggc acgattacaa acttaacagg tacagattta
     6001 acacttgtta gtggttccac taaagaatta ggtgttgtgc cacttaactt tagttatgat
     6061 ggccttactg caagtgttaa tataactgtg actaatgtcg gaagttatac tggagccgtt
     6121 gcaaaagatc cgctaacaat taaaggtgat tcatttgctc cagcaatttc aacaacaacg
     6181 ggcacaaatt atagtagttt atcagcatcc tgttatactt ttgctggtgt tggattacgt
     6241 aatttagatt cgtatattat ggtctacact agtggtcact tccgcaatgt ttcagcactt
     6301 gaaacgatta aatcacttaa attagactat aaaactggtg gaagtagtgc ttcagtacaa
     6361 gcttttagat ttggtacaac tgaaatcaca agtgcaagtg gtgcagcaca cacgacttta
     6421 acttcatcag ctggaggcac aagcgcaacg gtaaacgctc cagcgggtac aacacacttt
     6481 agaattgatg taagtagcaa aaacttacaa gcaaatataa cgattacctt tgaatcaggt
     6541 agtacataca catttacagc tgcagaacaa gcagcggcat acgaagatta tttcatggct
     6601 gtaactcacg aagagtgtct tgtcactgat gtaaaactta caacatggaa tcatttaaaa
     6661 actgaatttg atgcgatgct acctgaagct caaaatttaa ttagaacgac cgctagcgtg
     6721 gagtttaatg ctcgttataa cattattgtt gcaggttatg ggtacactaa tttcttagtt
     6781 agtggtggta cgggactagg tcgcgatctt aaaaatttcg aaaacaagct accaattcta
     6841 ataatgggat taggattacc attatcagtt tgtttttata ttgcacttaa aaaaagaaaa
     6901 aaaacattat aatttaagta aggttatgag aaaaagaaat tctataaaat tattacctat
     6961 tttagcgtca ctacttatta gtggttgtcg aactgagtta agtagtagcg aaaaaagtat
     7021 aagcgaagga acaagtatta gttctgaaat tgtttcaagt gaaactacaa gtgtgacgac
     7081 ttctagcgaa ga
//
LOCUS       DAOSLP010000044         6941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_9896_length_6941_cov_9.377142, whole genome shotgun sequence.
ACCESSION   DAOSLP010000044 DAOSLP010000000
VERSION     DAOSLP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9896_length_6941_cov_9.377142"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..924
                     /locus_tag="PK460_04325"
     CDS             106..924
                     /locus_tag="PK460_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54333.1"
                     /translation="MKKILGAIGGFFVAIWRWIKETAWVQPLLIVGIIFGIIFAIPSV
                     VDGIRKIDERNNSAEKYYQQFQVSLAGAENSAADKLLDEIKQNSEGGSESLKGQKFFV
                     VFVQKDEACSACLDAREGFEYLADDGKALLDDGRKIELKTIFVDQELKRKDKEDWKKE
                     DSDPVDNYAETAFEAFLLRNAARFEEYAGDALNTHYYINGGITEQQVEDIESADVKRF
                     QTPTILQIDFTDTAPQPGVTNVFIGVQGAKKLDRAKYIADAWNYKGQFGPNYTV"
     gene            complement(992..1417)
                     /locus_tag="PK460_04330"
     CDS             complement(992..1417)
                     /locus_tag="PK460_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOE54334.1"
                     /translation="MGLVLFQPMILGMAGNIGTQSMGVTILSLHSGEIKIRKHFGREL
                     LIGLLNSFASGIVGFGIVFLFLTILGQDNALMYAAVVGLSLMCAMIISSLAGVLTPII
                     LLAVKLDEKAASGPLITTINDFFALQIYFLIATLLFTVL"
     gene            complement(1507..1743)
                     /locus_tag="PK460_04335"
     CDS             complement(1507..1743)
                     /locus_tag="PK460_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE54335.1"
                     /translation="MDTNFPFVYEDEKFTRALKMIRDYDILMLPVLTRDGEMIGFVSE
                     TDALNLMAESHETMMRQLVKVAEDDDNLPPHKTP"
     gene            1790..1963
                     /locus_tag="PK460_04340"
     CDS             1790..1963
                     /locus_tag="PK460_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54336.1"
                     /translation="MVVAPTYLLFSSWKKITSISSLSAALAIVLYVAFLTVVVFGKEI
                     KCGELTAAALSAS"
     gene            complement(1965..2213)
                     /locus_tag="PK460_04345"
     CDS             complement(1965..2213)
                     /locus_tag="PK460_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54337.1"
                     /translation="MELNEKIIARLIEPADIAFLNQKQPFDLAVLYGELDEESQAYLL
                     SLLTDESRADLLSFLNPEDAAEFLEEMEIEEQQNILSY"
     gene            complement(2302..3615)
                     /gene="glmM"
                     /locus_tag="PK460_04350"
     CDS             complement(2302..3615)
                     /gene="glmM"
                     /locus_tag="PK460_04350"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOE54338.1"
                     /translation="MKLFGTDGIRGPANVLLTPGLSFDIGAFLGQYQKKNKILIAYDT
                     RRSGIVIFASLVAGILSSGGEASSIGVAPTPALAYLCKKYNYDYGIMISASHNPFQDN
                     GIKIFDTNGFKIAESIEEEIEKYIASDTRLKLKTGGEIGKFTDKSELIYEYVDYAKKA
                     FRGGLKLNLLIDGANGSASSVIHHIVAGTSLNATIINVEPDGVNINADCGSTHLDKLQ
                     DTIKKSPGKYDLGVAFDGDADRVLFVGPKGEVLDGDHVLYLLGKHYKKIGTLTKDTVV
                     ATVMANYGLGSALSKLGIKLVATSVGDKHVQREMIEHDYLIGGEQSGHTIFNGYIKTG
                     DGIYTMMKVLDVLAAEKTTFSEYVKEFIKFPQCLLNIRVKDKKMAMTDKTLLAEIKKS
                     EAKLGGHGRILVRASGTEELVRVMVEAETEAEAKATAEHLASFVK"
     gene            complement(3636..4409)
                     /locus_tag="PK460_04355"
     CDS             complement(3636..4409)
                     /locus_tag="PK460_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HOE54339.1"
                     /translation="MFLILSEMLSELQIQVIQLLVVFVFIVTLDVLLLITLKRIFPKV
                     TLIVLSVVLLVLGLFDLTYAFYLVALIYMLCAMTFIFGNIVEFRWLISNAATPKDRRF
                     QRDFRKIYDQEDLVNQLVSAVFSLSKTKTGALITIERTNQLTELIKNGSPIDAPVSAP
                     LLTTIFYKGTALHDGAVVIRDDTILAASVYFTPSTKALAGKVGSRHRAALGISEISDA
                     VTIVVSEETGRISIAYRAALQPVSPEDFRKVLVDHLLTK"
     gene            complement(4622..5503)
                     /locus_tag="PK460_04360"
     CDS             complement(4622..5503)
                     /locus_tag="PK460_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE54340.1"
                     /translation="MKTAMMTGHVTRFGDLEFALRLIKELGFEAVDLTMYDTFGGVAV
                     RNRELFTGDIIANAKRIKSIAEEINLPIVQAHTPFHVYRKDDEEYNAYMLETQKLCLQ
                     IAGIIGIPYVVIHPVMNADNEFNKKFFDELLPLAKKMNVVIATENMWEWQVGAIHCDY
                     STCSTPESFLSLLEYINHPNLKACVDVGHAQMFNGFDKSVSPKLMIEKLAHKYCVALH
                     LHDNDCVNDYHKIPFDGVINWEEVYDALTKINYQGHLVTETSLPREFNLEESKEFWLK
                     QREAADKIKSELLKRRA"
     gene            complement(5526..5972)
                     /locus_tag="PK460_04365"
     CDS             complement(5526..5972)
                     /locus_tag="PK460_04365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE54341.1"
                     /translation="MNKAESYFKRAQQEDGYLSFLFIDIDFFKKVNDTYGHLFGDEVL
                     KVVAKTIAENIRPLDLCCRFGGEEFLIYLHDTSKEEAITIGNRIREVVQLLEFKQVKE
                     YKCTISVGVYSAKPNTETNLQQFIDNVDQALYKAKASGRNCVMSFD"
     gene            complement(5969..6715)
                     /locus_tag="PK460_04370"
     CDS             complement(5969..6715)
                     /locus_tag="PK460_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54342.1"
                     /translation="MFLQNLLETLIKLDVKTMMGVLICGNLVLSLLTLRYYIYHDSNY
                     DKTIIQRFGIAKILQAIAWLFLFFRGEISDVISVYFGNILLFVSFYLDSLIVLRLNDK
                     WTKGWVITQSVLLAVAVILFLVFEITLSAAHIRVGIASLAVFLVFAAPNVLSILNKES
                     TKFQKVIAAISLLFLIFLLLRAFNSLFDNEIVLFTNNFFQIGTFLALVLLMLTNGVGF
                     LLIMYDRSYALVKESSNLDPLTRIYNRRFL"
BASE COUNT         2319 a         1299 c         1084 g         2239 t
ORIGIN      
        1 aactttagta aattgctttt tttattattg cttttgtttg aaaatatatg ttgagagtgt
       61 tattattata cgtgctctat tatttaatag aaggaggaac ttttcatgaa aaaaatctta
      121 ggtgctatcg gtggtttctt tgtagctatc tggcgctgga ttaaagaaac agcctgggtc
      181 caaccacttt taattgttgg aattatcttt ggaattattt ttgcaattcc aagtgtcgtc
      241 gatggcattc gtaaaattga tgaacgtaac aatagtgcgg aaaaatacta tcaacaattc
      301 caagtctcac ttgctggtgc ggaaaatagt gccgctgata aattacttga tgaaattaag
      361 caaaattcag aaggtggtag tgaaagctta aaaggacaaa aattcttcgt agtttttgtg
      421 caaaaagatg aagcatgcag tgcatgtctt gatgctcgtg aaggctttga atatttagcg
      481 gatgatggta aagctttact tgatgatggc cggaagattg aacttaaaac aatctttgtg
      541 gaccaagaat taaaacgtaa agataaagaa gattggaaga aagaagatag cgatccagtt
      601 gataattatg ctgaaaccgc ttttgaagct ttcttacttc gtaacgctgc tcgttttgaa
      661 gagtatgctg gggatgcact taatacccac tattatatta atggcggtat tactgagcaa
      721 caagttgaag atattgaatc agcggatgtc aaacgtttcc aaaccccaac catattacaa
      781 attgacttta ctgacacagc accacaacct ggtgttacaa atgtctttat cggtgttcaa
      841 ggagcgaaaa aacttgatcg cgctaaatac attgctgatg catggaacta taaaggtcaa
      901 tttggaccaa attatactgt ctaacattat taaaaagttg aaaaatgccg gcgttaagcc
      961 ggcttttctt tttacttctt ctactaaatt attaaagtac tgtaaacaaa agtgtcgcga
     1021 ttaagaaata aatttgtaag gcaaagaagt cattaatcgt tgtaataagc ggaccagacg
     1081 ctgctttttc gtctagctta acagcgagca aaataattgg cgttaacaca cctgctaatg
     1141 agctaataat cattgcgcac ataagtgata agcctactac tgcagcatac atcaaagcat
     1201 tatcttgacc taaaatagta aggaataaga aaacaatacc aaacccgacg ataccggaag
     1261 caaaactatt taataatcca attaaaagtt cacgcccaaa atgttttcta attttaattt
     1321 caccactatg taagcttaaa attgtgaccc ccatactttg tgtaccaata ttaccagcca
     1381 tcccaagaat cattggttgg aataagacta agcccacaaa agtacttaaa agtccttcgt
     1441 atgatgtaag tgtgccagcg attagtaagt taagtacgac cgcgacgagc ggccacggta
     1501 accgtgttaa ggcgttttat gtggtggaag attatcatca tcttcggcaa ctttcactaa
     1561 ttggcgcatc attgtttcat gcgattccgc cattaaattt aatgcgtcag tttcagaaac
     1621 gaacccaatc atttcaccat cacgcgttaa tacaggaagc attaatatgt cataatcacg
     1681 aatcattttt aatgccctag taaatttttc gtcttcatac acgaacggga aattagtgtc
     1741 gataacttca ctaattggag ttgttgaacg agcggcaatt aagtctttaa tggtagtagc
     1801 ccctacatat ttattatttt catcatggaa gaaaataaca tcaatttcat cattatcagc
     1861 ggcattagca atcgtattat acgttgcttt tttaactgtt gtcgtatttg ggaaagaaat
     1921 aaagtgtggc gagctaaccg cagctgcact atcagcgtcg taacttagta acttaaaata
     1981 ttttgttgtt cttcaatctc catttcttcc aaaaattcag cagcgtcttc tggattaagg
     2041 aagcttaaaa gatcggcgcg tgattcatca gttaataaac taagtaaata tgcttggctc
     2101 tcttcgtcta attcaccgta aagaaccgct aagtcaaatg gttgtttttg attaagaaaa
     2161 gcaatgtcag cgggttcaat taaacgtgca attatctttt catttaattc cattgacatg
     2221 ccccctttca taaataaaat tataccccat tatttaggat gtgaattaaa taattataat
     2281 tttttaattt acaacttaca tctattttac aaaagacgct aagtgttctg ctgttgcttt
     2341 tgcttccgct tcggtttcag cttcaaccat cactcttact aattcttcag ttccgctagc
     2401 gcgaactaaa atacgaccat gaccaccaag tttagcttca ctctttttaa tttccgcaag
     2461 caatgtttta tcagtcattg ccatcttttt atctttgact ctaatattta ataaacattg
     2521 tggaaatttg ataaattctt taacatattc actaaatgtt gttttttctg ccgctaagac
     2581 atctagtact ttcatcatcg tataaatgcc gtcacccgtc ttaatataac cattaaatat
     2641 tgtatgacct gattgttcac caccaattaa gtaatcgtgt tcaatcattt cccgttgaac
     2701 gtgtttatcg ccaacactag tggcgactaa tttaatacct aatttactta atgctgaacc
     2761 aagtccgtaa tttgccatca ctgtggcaac gacagtatct tttgtcaatg tgccgatttt
     2821 tttataatgt ttccctaaaa gatataatac atggtcacca tcaagtactt cacctttggg
     2881 tccaacaaat aaaacacgat cagcgtcacc atcaaaagca acaccgaggt catatttacc
     2941 aggtgacttt ttaattgtgt cttgtagttt atctaaatgg gttgaaccgc agtctgcatt
     3001 gatattaaca ccgtctggtt caacattaat aattgtggca tttaaactag tgcctgcaac
     3061 gatatgatga ataacactgc tagcgctccc attagcgcca tcaattaata aattaagctt
     3121 gagtccaccg cgaaatgcct tcttagcgta atcaacatat tcataaatta actcggattt
     3181 gtcggtaaat ttgccaattt caccgcctgt tttaagttta agtcgtgtgt cacttgcaat
     3241 atacttttca atttcctctt cgattgattc agcaatttta aaaccatttg tgtcaaatat
     3301 tttaatgccg ttatcttgga aaggattatg cgatgcacta atcatgatgc cgtaatcata
     3361 attgtatttt ttacataaat acgctaaggc aggtgttggc gcaacaccaa tggaactagc
     3421 ctcaccccca ctgcttaaaa tacccgcaac taagctagca aaaataacaa ttccacttct
     3481 gcgcgtgtca taagcaatta aaatcttatt tttcttttgg tattgaccta aaaaagctcc
     3541 aatatcaaaa cttaagcctg gcgtcagtaa aacatttgcc ggtcctctaa ttccgtctgt
     3601 tccaaataat ttcatatata acctctttaa ataatttatt tcgttaataa atgatcgact
     3661 aatacttttc taaagtcttc aggactcaca ggttgcaaag cagcgcgata agcaatggaa
     3721 atacgaccag tttcttcact aacaacaatc gtaacagcat cactaatttc gctaattcct
     3781 aatgctgcgc ggtgccgtga gccaaccttt cctgcaagtg ctttggttga tggtgtaaag
     3841 tagacactag ccgctaaaat ggtatcgtca cgaattacaa cggcaccgtc atgaagtgcc
     3901 gtccccttat agaaaatagt cgttaataaa ggtgcactaa ccggtgcatc aatcggactg
     3961 ccatttttaa ttaattccgt taattgatta gtgcgttcaa tagtaataag cgcaccagtt
     4021 ttagtttttg ataacgaaaa taccgcagat acaagttgat ttactaaatc ctcttggtcg
     4081 taaatcttac gaaaatcacg ctggaagcga cggtcttttg gtgtggcggc attggagatt
     4141 aaccatctaa attcaacaat attaccaaaa ataaacgtca tagcgcataa catataaatt
     4201 aaagcaacta agtaaaaggc atatgttaaa tcaaataaac cgagcactaa caagacaact
     4261 gaaagtacga taagcgtaac tttggggaat atacgtttaa gcgtaattaa taaaagtaca
     4321 tcaagtgtaa ctataaaaac aaaaacaact aagagttgta taacctgaat ttgcaattca
     4381 ctaagcattt ctgaaagtat taaaaacata ataaatcccc cgtctatttg tataaatatt
     4441 ttagcatgta aacacaacca ttttcaattt aaaatgcgta aattgtcgct attttttaac
     4501 gaaaactaat cccaaattaa aaagagtgcc attttgcaag gttatcgcaa taaacattat
     4561 aattatttcg gcaatataat actaaacatt atgttcgaat gttatttaaa atatcagagc
     4621 attaagcgcg tcttttaagt aattcacttt taattttgtc cgcagcttct ctttgtttaa
     4681 gccaaaactc tttactttct tcgaggttaa attcacgggg taaactcgtt tctgtaacaa
     4741 gatgaccttg atagttaatc tttgttaaag catcataaac ttcttcccaa ttaattacac
     4801 catcaaacgg aattttatga taatcattaa cacaatcatt atcatgaaga tgcagtgcaa
     4861 cacaatattt atgagcaagt ttttcaatca ttaatttcgg tgatacactt ttatcaaaac
     4921 cattaaacat ttgcgcatga ccaacatcaa cacacgcctt taagttagga tgattaatat
     4981 attctaaaag gcttaaaaat gattcaggag ttgaacaagt tgagtaatca caatgaattg
     5041 ctcctacttg ccattcccac atattctcgg ttgcaatgac aacattcatt tttttcgcta
     5101 gcggaagtag ttcatcaaaa aactttttat taaactcatt atcagcgttc atcactggat
     5161 gaataacaac ataaggaatg ccaatgatgc ccgctatttg taaacataat ttttgtgttt
     5221 ctaacatgta tgcattatat tcttcatcgt ccttacgata aacatgaaac ggggtatgtg
     5281 cttgcacaat cggaagatta atctcttcag cgatactttt aatgcgtttc gcgtttgcaa
     5341 ttatgtcacc agtaaataac tcacgattac gaacagcaac accaccaaag gtgtcataca
     5401 ttgttaaatc aacagcttca aaacctaact ccttaataag tcgtaaagca aactctaaat
     5461 caccaaagcg tgtcacatgg cctgtcatca ttgctgtttt cataactttg ctcctctata
     5521 tttatttaat caaaactcat tacacaatta cgaccagaag cttttgcttt atataaagct
     5581 tggtcaacat tatcaataaa ctgttggagg tttgtttcgg tgtttggctt agcggaatat
     5641 acaccgacac taattgtaca tttatattct ttaacttgtt taaattctaa tagttgcact
     5701 acttcccgaa ttctattacc gattgtgatc gcttcttctt tactagtgtc atgtaaataa
     5761 attaaaaact cttccccgcc aaatctgcag caaagatcta atggtcgaat gttttctgcg
     5821 attgtctttg ctacaacctt taatacttcg tccccaaata agtgtccata agtatcattt
     5881 actttcttga aaaaatcaat atcaataaaa agaaatgata agtaaccatc ttcttgctgt
     5941 gcccgcttaa aataggactc agctttattc ataaaaagcg ccgattataa atgcgtgtta
     6001 acggatctaa attagaactt tctttcacta atgcataaga gcggtcatac attataagta
     6061 aaaatcctac gccattagtt aacattaata acactaaggc aaggaatgta ccaatttgga
     6121 agaaattatt agtgaaaagt acaatctcat tatcaaatag ggaattaaat gctcgtaata
     6181 agaggaagat aaggaagagc agacttattg cggcaatgac tttttgaaac ttcgtgctct
     6241 ctttatttaa aatacttaaa acattagggg cggcaaaaac taaaaatacc gctaaagaag
     6301 caatcccaac cctaatatgc gcagcgctaa gcgtaatttc aaaaactaaa aataagataa
     6361 ctgctaccgc taataaaaca ctttgtgtta tcacccagcc ctttgtccac ttatcattta
     6421 gccgtaaaac aattaacgaa tctaaataaa aactgacaaa gagcaatata ttaccaaagt
     6481 aaacagaaat aacatcactt atttcccctc taaaaaataa aaataaccac gcgattgctt
     6541 gtaaaatttt agcaatacca aatctttgga ttattgtttt atcataattt gaatcgtgat
     6601 atatgtaata acgaagtgtt aaaagtgata acactaaatt gccacaaatt aaaacaccca
     6661 tcattgtttt tacatctagt ttaataaggg tttctaataa gttttgtaag aacatcttct
     6721 attggcctct ttctttttaa ctctgatcaa tatgcatcct ttttttaatg cataatcaag
     6781 gctttgtata tattatacaa aaattaatat ctcgcgcctt agaaaggtac aaaatagtga
     6841 tattttttta caattaaatg atatttttat aattatttaa tcaactttta catgttgttt
     6901 tattacaaaa aaatattatt tattctaaaa cttgacattt a
//
LOCUS       DAOSLP010000045         6855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_10053_length_6855_cov_9.945294, whole genome shotgun sequence.
ACCESSION   DAOSLP010000045 DAOSLP010000000
VERSION     DAOSLP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10053_length_6855_cov_9.945294"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1550
                     /locus_tag="PK460_04375"
     CDS             135..1550
                     /locus_tag="PK460_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE54343.1"
                     /translation="MDYDVIIVGAGPAGYFCAYELTEKRPNLKVLLIEKGRAINTRSC
                     PILKELIDKCPINKEGFQECYPSCSITCGFGGAGAYSDGKFNITSEFGGWMQDYLERE
                     ELLDLINYVDSINLKFGASNEITDPYTNKVKEIELRAMSVGVRLLRSKVRHLGTEENF
                     KILGRIFKHLQKKVTMLFETDVVEIITENNEIRGVVTKDNEQFTAPKVLLAVGREGSK
                     WLDTILEKRGIKMDQTQVDIGVRVECNDLIMEEINKHLYEGKFVYRTSTGNTVRTFCS
                     NPSGHVVMENYNGVINVNGHSYKDEAFRSENTNFALLVSHHFAEPFNKPNEYAVKVSS
                     LANQLACGSVLVQKFGDLLLGRRTTVKRLNEGFMRPTLKEAVPGDLALCLPHKTLQSI
                     IEMIRVLDKITPGIATEHTLLYGVEAKYYSARPEMTTEFETKAIKGLYLGGDGAGLTR
                     GLAQAGACGVYIARNMLKELK"
     gene            1552..2745
                     /locus_tag="PK460_04380"
     CDS             1552..2745
                     /locus_tag="PK460_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOE54344.1"
                     /translation="MRKVGTSARGIRCPIIRSGDDLAQVVVNSVLEAAKAEGFTLRDR
                     DIIGITESLLARSQGNYASIDAIASEIKAKFPGGELGVIFPILSRNRFATLLKGIARG
                     AKKIYLMLSYPRDEVGNALLSEALIEKAGVNPYTDILTVPEYRKLFGYPKHEFTGIDY
                     VELYTDIVEQESCKIELIFANHAQMILNYTKHVLTCDVHTRQDSKHKLKVAGAETIYG
                     LDDLLTTPQLGEKGFNPQYGLLGSNKATDDSIKLFPRDGQPVVEKIQQMLLTKTGKHI
                     EVLIYGDGAFKDPQGHIWELADPVVSPFFTPGLSGTPSEVKLKYLADNDFKNLTGAEL
                     TKAIAASIKAKPKDLVGTKATLGTTPRQITDLVGSLCDLVSGSGDKGTPVVLIQGYFD
                     NYTDN"
     gene            complement(2746..3285)
                     /gene="hpt"
                     /locus_tag="PK460_04385"
     CDS             complement(2746..3285)
                     /gene="hpt"
                     /locus_tag="PK460_04385"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOE54345.1"
                     /translation="MMMKDLEKVLVTEAEITEIINRVSARIEKDYKGKDLVMIGILKG
                     GAPFMMDLIKKINLPLIIDFIRVESYFGGTTQGELVFRKDIEVDITNKHVLIVDDIID
                     SSRTLKMVIDLFKKRNASSIEVCTLLDKPSGRVIDYTPKYIGTTIGNEFVVGYGLDYD
                     EKYRNLPVIGVLKEEVYKK"
     gene            complement(3285..4766)
                     /locus_tag="PK460_04390"
     CDS             complement(3285..4766)
                     /locus_tag="PK460_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOE54346.1"
                     /translation="MIAFDYEHYLTIQKREILKRIKLFDNKLYLEFGGKLIEDNHAAR
                     VLPGFHPSGKLEIFSSLKEQIEVIIVVNTNDIVANKIGSDNNISYQGEVERLIDEFRT
                     KDIAVCGVVFSFFSESIVTNAFIKKLARNNIKTYKHYVIEDYPHNLEKIFSEDGLGRN
                     EYVETTRPLVVVTAPGSGSGKLATCLSQLYHDNKNGIRAGYAKYETFPIWNLPLNHPL
                     NLAYEAATVDLGDFNVIDPYYLDKFNVKATNYNRDVDAFPVLKAIFEKMYGETPYESP
                     TMMGVNMVGFALGDEEAITAACKEEVIRRFYSAQKNLFLDKYTASSLDKIRMIMNKLG
                     IVETDRKCVLPALKKADETHVPCMALVLNDGKIITAKTSKLLSPAAALFLNSVKYLAN
                     INDEIPLISPNLIEPIEALKKEISAINDSVLNLNDTLISLAIQAPMNPLSDLALKQIE
                     KLKGAQAHSSVLLDKRDLQVLKQLGINVTEESVPASNKLYLRS"
     gene            complement(4782..5750)
                     /locus_tag="PK460_04395"
     CDS             complement(4782..5750)
                     /locus_tag="PK460_04395"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOE54347.1"
                     /translation="MSFSKDACIFSLTANKELTREVAKILGVNVSEAIVTHFADGETL
                     CEPTSSFRGKNCYVIQSTCKPVNERLMEILIFADALKRASAKSINVVMPYFGYARQDR
                     KNKPRQPITGRLVADLIHAAGIDRVVVIDLHAPQIQGFFTCLVDEMTAIPFLAKYFKD
                     KVDDDWVIVSPDHGGMARARRVAEFLNKPLAIIDKRRPKPNEATISHVIGDVKGKHCL
                     VIDDIIDTGGSSRAGAIALLEHGAKSVRIAATHGVFSPGAEVKLYEDNIFKEIVVTDS
                     IPTPEHMKGKPLTVLSLALMIAKAIEHINSGKSLTIVYEMFSNTEE"
     gene            complement(5756..6382)
                     /gene="upp"
                     /locus_tag="PK460_04400"
     CDS             complement(5756..6382)
                     /gene="upp"
                     /locus_tag="PK460_04400"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOE54348.1"
                     /translation="MVFEIKHPLIDVKLSIMRDEKTKSKEFRESLDEIASLMSYEIFK
                     DIGTHASEDTFMTPTGVELQKIKLDNKIILVPILRAGVGMVDGIRNMIPTARIGHIGI
                     YRDEKTLEPHEYYFKLPKVDNPLVVLVDPMLATGGTAIASIDAIKARGYNNIRMATLV
                     ASPLGIERVSIAHPDVHIYVAAIDEKLNEHGYIIPGLGDAGDRIFGTR"
     gene            complement(6506..>6855)
                     /locus_tag="PK460_04405"
     CDS             complement(6506..>6855)
                     /locus_tag="PK460_04405"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HOE54349.1"
                     /translation="FNFYIFEYIGTLIICVIIINSSINIIKEAAYYLTDASPSKKTVE
                     SIRECILNVEGVICVDDLKVRRHMNNYYVDVEVGINKNLTLKEAHDISEKVHSQVEAN
                     FKVLHCHVHINPH"
BASE COUNT         2195 a         1315 c         1195 g         2150 t
ORIGIN      
        1 aaatgtaata aagcgttacc aatattatta aattcaacta acaatataag tttatttatt
       61 ataataaaaa gggaaaataa atttctagat acccctcaag gtaaaataat ataaattaag
      121 gagacgcttt tactatggat tatgatgtga ttattgtcgg tgctggacca gcaggttatt
      181 tctgtgctta tgaattaact gaaaaacgtc caaacctcaa agttttatta attgagaagg
      241 gacgagccat taatactcgc tcatgtccaa ttttaaaaga attaattgat aaatgtccga
      301 ttaataaaga aggctttcaa gaatgttatc cgtcttgctc aatcacttgt ggctttggtg
      361 gtgctggagc ctattctgat ggcaaattta atatcactag tgaatttggt ggttggatgc
      421 aagattattt agaaagagaa gaattgctcg accttatcaa ctatgttgat agtattaatt
      481 taaaatttgg cgctagtaat gaaatcactg atccctatac gaacaaagtt aaagaaattg
      541 aactccgtgc gatgagtgtg ggtgttcgct tactccgtag taaagtaaga cacttaggca
      601 ctgaagaaaa ctttaaaata ttaggccgta tttttaaaca tttacaaaag aaagttacaa
      661 tgttatttga aactgatgtt gtggaaataa tcactgaaaa taatgagatt cgtggtgtag
      721 ttacaaaaga caacgagcaa tttactgccc ctaaagtatt actcgcggtt ggacgagaag
      781 gtagtaaatg gctcgacact attttagaaa agcgcggcat caaaatggat caaacccaag
      841 ttgatatcgg tgtccgcgtc gaatgtaatg acttaattat ggaagaaatc aataaacatc
      901 tttatgaagg taaatttgtc tatcgtactt ctactggtaa tacagtccgc accttttgtt
      961 caaatccaag tgggcatgtc gttatggaaa attataacgg cgtcataaat gttaatgggc
     1021 actcttataa agatgaggca tttcgatcag aaaatacaaa ctttgcacta cttgtgtcac
     1081 accactttgc tgaaccattt aataaaccaa atgaatatgc cgttaaagta tcttcactgg
     1141 caaatcaact cgcttgtggt tcagtcttag tgcaaaagtt tggtgactta ttactaggaa
     1201 gacgcaccac agtcaaacgc ttaaacgaag gctttatgcg gccaacatta aaagaggcag
     1261 tgcctggcga cttagcgctt tgtttaccac ataaaacact gcaatcaatt attgaaatga
     1321 ttcgtgtgct cgataaaatt actccaggta ttgcgacaga gcatacacta ctttacggtg
     1381 ttgaagccaa gtattacagt gcccgtccag aaatgactac tgaatttgaa acaaaagcta
     1441 ttaaaggctt atatttaggc ggcgacggtg ctggacttac tcgcggctta gcgcaagctg
     1501 gagcatgtgg cgtttatatt gctcgtaata tgttaaagga attaaagtaa gatgcggaaa
     1561 gtaggtacaa gtgcacgcgg tattcgttgt cccattattc gcagtggaga tgacttggcg
     1621 caagttgtcg ttaatagtgt attagaagcc gcaaaagcgg aaggatttac tcttcgtgat
     1681 cgtgatatta tcggtatcac tgagtcactc cttgcgcgct cacaaggaaa ttatgcttca
     1741 attgacgcta ttgcatcgga aattaaagca aagttcccag gcggagaatt aggagttatc
     1801 ttcccgattc tttctcgtaa tcgttttgca acattactta aagggattgc gcgtggcgca
     1861 aagaaaattt atttaatgct ttcttacccg cgcgatgaag tcggtaatgc gcttctaagt
     1921 gaagcactca ttgaaaaagc tggggttaat ccttacactg atattttaac agtgccagaa
     1981 tatcgtaaac tcttcggcta tccaaaacat gaatttaccg gcattgatta tgttgaactc
     2041 tatacagaca ttgtggagca agaaagctgc aaaattgaat taatttttgc aaatcacgcc
     2101 caaatgattc taaattacac aaaacatgtc ttaacgtgtg atgttcatac ccgtcaagat
     2161 tcaaaacata aattaaaggt cgcgggagct gaaacaattt acggattgga tgatttattg
     2221 actacgccac aactaggaga aaaaggattt aatccgcaat atgggctcct tggttcaaat
     2281 aaagccacag acgactcgat taaacttttt ccgcgtgacg gacaacctgt tgtcgaaaaa
     2341 attcaacaaa tgttactaac aaaaacaggc aaacatattg aagttttaat ttatggcgat
     2401 ggtgctttta aagatccaca aggacatatt tgggaactag cagatcctgt agtgtcacca
     2461 tttttcactc ctggattaag cggcacacct agcgaagtaa aactaaaata tctcgctgat
     2521 aacgatttta aaaatttaac gggcgctgaa cttactaaag caattgccgc ttcaattaaa
     2581 gctaaaccaa aagaccttgt aggcactaag gcgacgctag gtactacccc acgtcaaata
     2641 actgaccttg ttggatcact ttgtgattta gtaagtggtt ctggtgataa aggcacacca
     2701 gttgtgttaa ttcaaggtta ttttgataat tacacagata attaattatt ttttataaac
     2761 ttcttctttc aatacaccga taactggcaa gttacgatat ttttcatcat aatctaaacc
     2821 gtaaccaaca acaaattcat taccgatagt tgtcccaata tactttggcg tataatcaat
     2881 tacgcgacca cttggtttat caagtagtgt acacacttca atcgaactag cgttacgctt
     2941 tttaaagagg tcaattacca tttttaatgt gcggctgcta tcaataatat cgtcaacaat
     3001 taagacatgc ttgttagtga tatcaacttc aatatcctta cggaagacta gttcgccttg
     3061 tgtcgtgcca ccaaaataag attcaacacg aataaagtcg atgataagtg gcaaattaat
     3121 tttcttaatt aaatccatca taaacggggc gccgcccttt aaaatgccaa tcatcactaa
     3181 atctttaccc ttataatcct tttcgatccg tgctgaaaca cgattaataa tttccgtaat
     3241 ttctgcttca gtcactaata ctttttctaa atccttcatc ataatcatga cctcaaatat
     3301 aatttatttg atgctgggac actctcttca gtaacattaa ttcctagttg ttttaaaact
     3361 tgaagatcgc gtttatcaag gagcacagaa gagtgcgctt gtgctccttt taatttttca
     3421 atttgcttaa gcgcaagatc cgataacgga ttcatcggcg cttggattgc tagagaaatc
     3481 aatgtatcgt ttaaattaag cactgaatcg ttaatcgcac ttatttcttt tttcagcgct
     3541 tcaattggtt cgataagatt tggggaaata agcggaattt catcattaat attcgctaaa
     3601 tatttaactg aatttaagaa taatgcagcg gcagggctta aaagttttga tgtttttgct
     3661 gtaataattt tgccgtcatt aagcactaaa gccatacacg ggacatgtgt ttcatcggcc
     3721 tttttaaggg caggcaatac acatttacga tcagtttcaa caatgcctaa tttattcata
     3781 atcatccgga ttttatcaag actactagcg gtatatttat caaggaagag gtttttttgc
     3841 gctgaataaa aacggcgaat aacttcctct ttacacgcgg cggtaatcgc ttcttcatcc
     3901 ccaagcgcaa agccaaccat attgacgccc atcattgtcg gtgattcata gggcgtttca
     3961 ccatacatct tctcaaaaat agcttttaac acagggaaag cgtcaacatc acgattgtaa
     4021 tttgtcgctt taacattaaa tttatctaag taataaggat caataacatt aaagtcacct
     4081 aaatcaacag tcgctgcttc ataggctaag ttaagcgggt gattaagcgg taaattccag
     4141 attgggaatg tttcatattt tgcatatccc gctcttatac cgtttttatt atcgtgatag
     4201 agttgcgata aacatgtcgc gagtttccca cttcctgaac caggtgcagt aacaaccaca
     4261 agcggacgcg ttgtttcgac atattcatta cgacctaacc cgtcttcact aaatatcttt
     4321 tctaagttat gcggatagtc ttcaatcacg taatgtttat atgtcttaat attatttctc
     4381 gctagttttt tgataaaagc attcgtgaca attgattcac taaaaaagga aaagacaaca
     4441 ccacaaacgg cgatatcttt agtgcgaaat tcatcgatta accgttcaac ttcaccttga
     4501 taagagatat tattatcact gccaatttta ttagcgacaa tgtcattagt gttgacaaca
     4561 ataattactt caatttgctc ttttaaactt gagaaaattt caagtttacc gcttggatga
     4621 aacccaggta aaacacgagc agcgtgatta tcttcaataa gtttgccacc aaattcgaga
     4681 taaagtttat tatcaaacaa tttaatccgc tttaaaattt cgcgtttttg aatagttaaa
     4741 taatgttcat aatcaaaagc aatcataaac tttaactcct tttattcttc ggtattacta
     4801 aacatttcgt agacaatcgt gagtgatttg cctgaattaa tatgttctat tgctttcgca
     4861 atcatcagcg caagagaaag gacggttagc ggttttccct tcatatgttc cggtgttgga
     4921 attgagtcag taacgacaat ttctttaaag atgttatctt catataattt aacttccgct
     4981 cctggtgaaa aaacaccatg cgttgccgca atgcgaacag acttcgcacc atgttctaaa
     5041 agggcgattg cacctgcgcg tgaactacca cctgtatcga taatatcatc aatcactaga
     5101 caatgtttac ctttaacatc accaataaca tgacttatag tcgcttcatt tggttttggc
     5161 cgccttttat caataatcgc tagtggttta tttaaaaact ctgccactct gcgcgcccgc
     5221 gccattccgc catgatcagg agagacaatt acccaatcat catcaacttt atctttaaag
     5281 tactttgcta aaaaaggaat agcggtcatt tcatcgacta aacatgtaaa gaatccttga
     5341 atttgcgggg cgtgtaaatc aattacgaca acacgatcaa ttccagcagc gtgaattaaa
     5401 tccgcgacaa gccgaccagt aattggttga cgtggtttat ttttacggtc ttgtctagcg
     5461 tagccaaaat aaggcatcac aacatttatt gatttggctg aagctctttt taaagcatca
     5521 gcgaaaatga gaatttccat caatcgttca tttactggtt tacaagtaga ttgaatcaca
     5581 taacaatttt tcccgcggaa tgaacttgta ggttcacaaa gtgtttcccc atctgcaaaa
     5641 tgtgtgacaa tagcttcaga aacatttact cctaaaatct ttgctacttc acgcgttaat
     5701 tctttattgg ctgttaatga aaatatacaa gcatctttac taaagctcat aatctctacc
     5761 tcgttccaaa aatccggtca ccagcatcac caagtccggg gatgatgtaa ccatgttcat
     5821 ttaatttttc atcaattgcg gcgacataaa tatgaacatc ggggtgcgct atgctcacgc
     5881 gttcaatccc aagcgggctc gccacaagtg ttgccatccg aatattgtta taaccacgag
     5941 ctttaatcgc atcaatcgag gctattgcgg tcccaccagt tgctaacatt gggtcaacta
     6001 acactaccag cggattatcg acttttggta atttaaaata atattcatgt ggttctaatg
     6061 ttttttcatc acggtatata ccaatatgac caattctagc agtaggtatc atatttctaa
     6121 tgccgtcaac cattccaacc cccgctctta agattggaac tagaataatc ttattatcta
     6181 acttaatttt ttgtaactct acgcctgtcg gagtcataaa tgtgtcctct gaagcgtggg
     6241 taccaatgtc tttaaatatt tcataagaca tcagcgaagc aatttcatcg agtgattcac
     6301 gaaattcctt agattttgtt ttctcatcac gcatgattga taactttaca tcaatcagcg
     6361 ggtgcttaat ttcaaaaacc ataagtccct cctattgaaa tcatacatgt atattatact
     6421 taatataaat ctaaaaaagg gtctttaact attaaaaatt ttgtaataag ttaaaaaccg
     6481 aataatcttt acaaataatc gttatttagt gggggttaat atggacatgg caatgtaaaa
     6541 ctttaaaatt tgcttccact tgactatgaa ccttttcact aatatcatgc gcttctttta
     6601 atgtcaaatt tttattaata ccaacttcca catcaacata atagttattc atatggcgtc
     6661 taacttttaa atcatcaaca catatgacac cttcaacatt taaaatacac tcccgaatgc
     6721 tctctaccgt cttcttactt ggtgaagcat cagttaagta ataggctgct tctttaataa
     6781 tattaatact tgaattaata atgatcacac aaataataag tgtcccaata tattcaaaga
     6841 tataaaaatt aaatt
//
LOCUS       DAOSLP010000046         6646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_10479_length_6646_cov_9.522227, whole genome shotgun sequence.
ACCESSION   DAOSLP010000046 DAOSLP010000000
VERSION     DAOSLP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10479_length_6646_cov_9.522227"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..258
                     /locus_tag="PK460_04410"
     CDS             91..258
                     /locus_tag="PK460_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54350.1"
                     /translation="MLAIRFADFYNVSLDYLFGRSERQLQISEKDIENLSEQDKNEML
                     AFIIKLINEKK"
     gene            complement(269..1477)
                     /locus_tag="PK460_04415"
     CDS             complement(269..1477)
                     /locus_tag="PK460_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOE54351.1"
                     /translation="MHINKITVVGLGYVGLAQALLFNEDYNVVGYDTDTKVVASLNRG
                     ILHIKDEDFARALARKKLMATSDITYAFQNSDLVIIATPTHFNEEKQTFDLSSIDETL
                     QKIIASNRSPLVIIKSTVNVGATRYFMEKYPSLKIFFVPEFLREGYALFDNYYPARII
                     VGYDKNDTNHRQIAIQIANLFKKYTKSIDDVPLLITGTLEAEAIKLFANSYLALRVSF
                     FNEIDTFALSKDLEVKDIIEGVGLDSRIGTHYNNPSFGYGGYCLPKDSKQLKAQFNLI
                     PEELISATVNANSVRKEFIANYIADIVKKSNNKDYVVGIYRLTMKKDADNMRFSSIID
                     VVRLLKEQNIKMIIYEPLLNETTYQGITLINNLETFKKESSLIVTNRMHSDLKDVTNK
                     IFTRDVYYRD"
     gene            1569..1952
                     /locus_tag="PK460_04420"
     CDS             1569..1952
                     /locus_tag="PK460_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOE54352.1"
                     /translation="MKNVYTNMCMVYSGDKILVLNRVKKDWPGLTFPGGHVEDGELFS
                     ESVIREMKEETGLTIRNPEYVGQIVWPLPEQGINDVALLYRTNEFAGELLESVEGHVF
                     FIKKSELGNYQHSLDFDKVLELMFK"
     gene            complement(1958..2833)
                     /locus_tag="PK460_04425"
     CDS             complement(1958..2833)
                     /locus_tag="PK460_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54353.1"
                     /translation="MAIMSQLFNSYRFFEGIADMLGGIDFISYIFGFLSQFPIVVDII
                     IASAIIAALLRGIFKRFWKVVWRGVIFVILLIVLFATAEKLAPYIGSIPIPLRGTVNG
                     NAVEYANLGQIIYGVSIEAGNSETYAFEFTKLALKNLVVFFGMPALSLITPAISAITY
                     PLFSLLLPRKLKDAKLILPRIAISLGLTFIALVIFMAPAANFVPAMSAVKETMSEGTL
                     LYKFLNPEVINFLELFTPQKSVMLKIVDIGNISGKIKIFEEFTVDGVVIQLKDAVEAL
                     LEALKTIPYEAPAAG"
     gene            complement(2940..4235)
                     /gene="eno"
                     /locus_tag="PK460_04430"
     CDS             complement(2940..4235)
                     /gene="eno"
                     /locus_tag="PK460_04430"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOE54354.1"
                     /translation="MPTIIDVYAREVIDSRGNPTIEVDVTTESGAFGRAIVPSGASTG
                     EREALELRDGDKSRYLGKGVLTAVENVNKIIAPEIIGMDADDQVGIDKVLLELDGTPF
                     KKKLGANALLGVSLAAARAAADYFGVSLYNYIGGVNAKMLPVPMMNVINGGAHADSSV
                     DFQEYMIMPVGAPSLREALRWGAEVFHNLKKVLKAKGHITAVGDEGGFAPNLSSNEEP
                     LQVIVEAIKKAGYVPGKDFVLALDVAASEFFDEKTKKYVLKKSGGAVKTSEEMVTWYE
                     ELCEKYPIISIEDGLAEGDWDGWKLLTDRLSHKIQIVGDDLFVTNPAILKEGIEKGIA
                     NSILIKLNQIGTLTETLDAMQMAERAGYTCVVSHRSGESEDTTIADLAVALNAGQIKT
                     GSMSRTDRIAKYNQLIRIEDDLGERAIYPGLSAFYNLRK"
     gene            4392..4796
                     /locus_tag="PK460_04435"
     CDS             4392..4796
                     /locus_tag="PK460_04435"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOE54355.1"
                     /translation="MEQKEIKNVVAALIFYDGKLFAASRSEKDERYGKYEFPGGKIEL
                     EENPSSAIVREIKEELMMDISVKDLFMHVSHEYPWFKLEMDVFICEVTNPQFTLLVHK
                     DGGFFTKEEILKLPFLGADLQVLAEISKRLDF"
     gene            complement(4793..5383)
                     /gene="rdgB"
                     /locus_tag="PK460_04440"
     CDS             complement(4793..5383)
                     /gene="rdgB"
                     /locus_tag="PK460_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOE54356.1"
                     /translation="MKKQIVIATNNANKLKEFKSLFANTPYEVLSLADINFTGVIKED
                     GESFSDNAVIKAMTIHKLTNLPVIADDSGLSVNALEGFPGVNSARFMEGSPYTEKNAA
                     IIKMLEGHSDKSAKFICAIALVGLDSFPQVFIGEVKGTIINEARGEEGFGYDPIFFHP
                     NSQKTFAEMTLEEKDEISHRGRATALLFEYLETHYF"
     gene            complement(5388..6452)
                     /locus_tag="PK460_04445"
     CDS             complement(5388..6452)
                     /locus_tag="PK460_04445"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOE54357.1"
                     /translation="MSNLVGYSIVALFVIVMGLLLLLKVYLQTYHPGKYWYIERPIKY
                     LMILGPMFFLFAIGERWHFGENFLPSKNPDDLAWGPFHLGWLFAMVIAIIVVSSGVKA
                     DKANTKRYVFGQLNKIDFTVFQFGVLLFGIELYKQLIFLNLYEGLANYHWYGFPLQFC
                     SIPIFLYPLTPFIKNEKIKEAIYSFISIFNLIGGLAVMILATGVYTLQVSISIHTMIW
                     HGVMVVVAFYLINAYKIGTKWRHYLGAVTVLFCLIVLAQLTNILFHYIGMKFPGPGDF
                     DGFFISPWIDRRNMPILGDIRANMIAGGVPTLIIALVFPHIYFVIFSLTGLLIYYLFH
                     FIWKDVEKNKKEKALKTNTL"
BASE COUNT         2206 a         1199 c         1118 g         2123 t
ORIGIN      
        1 cgcctcaaaa agtagtttta cactagaaaa ttggcttaag tccaaaagtc aggttctaaa
       61 atagaacgcg gcataactcg gcttgatcca atgctagcaa ttcgttttgc agatttttat
      121 aatgtttcac ttgattactt gtttggccgt agtgaaaggc agttacaaat tagcgaaaaa
      181 gatattgaaa atttatcaga acaagataaa aatgagatgt tagcgtttat tattaaactt
      241 attaacgaaa agaaataatg aagatatatt aatcgcgata atagacatcg cgggtgaata
      301 ttttgttagt gacatctttt aagtcactat gcatacggtt tgtgacaatt agtgaacttt
      361 cttttttaaa tgtttctaaa ttattaatga gcgtaatacc ttgataagtt gtttcgttta
      421 aaagcggttc ataaataatc attttaatgt tttgctcttt tagtaatcta actacatcaa
      481 tgattgatga aaagcgcata ttatcagcat cttttttcat tgttaagcga taaataccaa
      541 cgacataatc tttattattg ctctttttta caatgtcggc gatataatta gcgataaact
      601 ctttacgcac actatttgcg ttaacagtcg cactgattaa ttcttcggga attagattaa
      661 attgtgcttt tagttgttta ctatctttcg gtaaacaata accaccataa ccaaatgaag
      721 gattattgta atgtgtccca atgcgcgaat ctaagccaac accttcaata atatctttaa
      781 cttctaagtc tttagataac gcaaatgtat caatttcatt aaagaaagat acgcggagcg
      841 ctaaataact attagcgaat aatttaattg cttcagcttc taaagtgccc gtaattaaaa
      901 gcgggacatc atcaatagat ttagtgtatt ttttaaagag attagcaatt tgaatagcaa
      961 tttgccggtg gtttgtgtca tttttatcgt agccgacaat aattcgcgct ggataataat
     1021 tatcaaaaag ggcataacct tcgcgtaaaa attcagggac aaaaaagatt tttaatgatg
     1081 gatacttttc cataaaatac cgagtcgcgc caacattaac cgttgattta ataataacaa
     1141 gcggactcct attacttgcg ataattttct gtaaagtttc atcaattgat gagagatcaa
     1201 atgtttgttt ttcttcatta aaatgggtag gtgtcgcaat aataacaaga tcactatttt
     1261 ggaatgcata agttatgtca cttgttgcca ttagtttttt acgagcaagc gctcgcgcaa
     1321 agtcttcgtc tttaatatgt aaaataccgc gattcaaact agcgacaact ttcgtatctg
     1381 tgtcataacc aacaacatta tagtcctcat taaaaagtaa ggcttgcgct aagccgacgt
     1441 atcccaatcc aacaactgta attttattaa tatgcatatg cttcccttaa attataacat
     1501 ttatcaaatt attcactaat gcagagtacc ttttttctaa aacttttgaa ttatggtacg
     1561 attttaatat gaaaaatgtt tacacaaata tgtgcatggt ttattcaggt gataagattt
     1621 tagtgcttaa ccgtgttaaa aaagattggc cgggccttac ttttccaggt ggccatgttg
     1681 aagatggtga attatttagt gaaagcgtaa ttcgtgaaat gaaagaagaa acaggtttaa
     1741 caattcgtaa tccggaatac gttggtcaaa tcgtctggcc gcttcctgag caagggataa
     1801 atgatgttgc ccttttatat cgcacaaatg aatttgctgg tgaactttta gaaagtgttg
     1861 aaggtcacgt ctttttcatt aaaaaaagtg agcttggtaa ttatcaacac tcacttgatt
     1921 ttgacaaagt tctagaacta atgtttaaat aataagatta tcctgctgct ggagcttcgt
     1981 aaggtatagt tttaagtgct tcaagtaagg cttcaacggc atcttttagt tgaataacaa
     2041 ccccatcaac agtaaattct tcaaagattt tgatcttgcc agaaatattt ccaatatcaa
     2101 caatttttaa cattactgat ttttgtggtg taaataattc taagaaatta ataacttcgg
     2161 gatttaagaa tttgtagagc aatgtgcctt cactcattgt ttctttgaca gcactcatcg
     2221 ctggtacaaa gtttgcggcg ggagccataa agataacaag ggcgataaag gttaagccaa
     2281 ggctaattgc aatacgcgga aggatgagtt tagcgtcttt taacttacgt ggaagtaata
     2341 aactaaataa cggataggta attgctgaaa ttgccggtgt tatgagtgat aaagcgggca
     2401 tcccaaagaa aacaacaaga tttttaagcg ctagttttgt aaattcaaaa gcataggttt
     2461 cactattacc tgcttcaatt gaaacaccat aaattatttg tcctaagttt gcatactcaa
     2521 cagcgttgcc atttactgtc ccgcggagcg ggataggaat actaccaata taaggtgcaa
     2581 gtttttctgc tgttgcaaat aagacgatta aaagaataac aaagatgaca ccacgccaga
     2641 cgactttcca aaatcttttg aaaataccgc gtagcagcgc agcgataatc gcacttgcaa
     2701 tgatgatatc aacgacaatt gggaactggg ataaaaaacc aaaaatatat gaaataaaat
     2761 ctattccacc aagcatatcg gcaatacctt caaaaaagcg ataagaatta aagagttgac
     2821 tcattattgc cataagatta cctcctgctt aaattataca taattaataa ttaaaaaaat
     2881 atagatttat aaaaaaagag ggtttcccct cttttaatgt gacaagtaat attacgcaat
     2941 tatttgcgta aattatagaa agcacttaaa ccaggataga ttgctctttc accaagatcg
     3001 tcttcaatgc gaattaattg gttgtattta gcaatccggt cagtacggct catcgaacca
     3061 gttttaattt gaccagcgtt taaagcgact gctaagtcgg caatagttgt atcttcactt
     3121 tcaccagaac ggtggctaac aacacaagtg tacccagcgc gttccgccat ttgcatagcg
     3181 tctagtgttt cagttaaggt accaatttga tttaatttaa taagaatgga gtttgcgatg
     3241 cctttttcaa taccttcttt aagaattgct gggtttgtaa cgaataagtc gtcaccaaca
     3301 atttgaatct tatggcttaa acggtcagtt aaaagtttcc agccatccca gtcgccttct
     3361 gctaaaccgt cttcaatcga aataattggg tacttttcac ataattcttc gtaccatgta
     3421 accatttctt cacttgtttt aacggctccg cctgatttct taagcacata tttctttgtt
     3481 ttttcatcaa agaattcact cgccgcgacg tcaagcgcta gaacaaagtc tttacctgga
     3541 acgtatcctg cttttttaat agcttcaacg attacttgta aaggttcttc gttactactt
     3601 aagtttggtg caaagccgcc ttcatcccca actgcggtaa tatgaccttt ggcctttaaa
     3661 actttcttta agttatggaa aacttcagcg ccccagcgga gtgcttcgcg taagcttggt
     3721 gccccaactg gcataatcat atattcttgg aaatcaacac tgctatcagc atgtgcgcca
     3781 ccgttaataa cattcatcat tgggactggt aacattttgg cattaacacc accaatgtag
     3841 ttatataaac ttacaccgaa ataatctgca gcggcacgag cagcagctaa actaacacca
     3901 agtaatgcgt tagcgcctaa tttcttttta aatggggtac catcaagttc taataaaact
     3961 ttatcaattc ccacttggtc atcggcatcc attccgataa tttctggagc aataattttg
     4021 ttaacatttt caacagcggt taacactcct ttgccgagat agcgactttt atcaccatca
     4081 cgtaattcta atgcttcgcg ttcacctgta ctagcgccac ttggaacaat tgcacgtcca
     4141 aaagcaccag attcagtcgt aacatcaact tcgatcgttg ggttaccacg tgagtcgatt
     4201 acttcacgag catacacatc aataattgtt ggcatgtatt tttcctcctc tttgtaaata
     4261 gccacatata ttataatatt ttttctgctt gctttgtagt aaaaagaact aaagacatcg
     4321 ttttatcagt aaatatatcc cgatatcaat ttacagcgta aaaatatgtt actttgttat
     4381 actaataaag tatggaacaa aaagaaatta aaaatgttgt ggcggccctt atcttctatg
     4441 atggtaaact tttcgctgct tcacgaagtg aaaaagatga acgctacggt aaatatgaat
     4501 ttcccggtgg aaaaattgaa ctagaagaaa atccaagttc tgctattgta cgcgaaatta
     4561 aagaagaatt aatgatggat ataagtgtca aagacttatt tatgcatgta agtcatgagt
     4621 atccatggtt taaacttgaa atggatgttt ttatttgtga agttactaac ccgcaattta
     4681 ccttattagt gcataaagat ggtggttttt ttacaaaaga agaaatatta aaattgccct
     4741 tcttaggggc tgatcttcaa gtgcttgctg aaattagtaa gcgtttagac ttttaaaaat
     4801 aatgcgtttc taaatattca aatagtaatg ccgtcgccct tccgcgatgg ctaatttcat
     4861 ctttttcttc aagcgtcatt tcggcaaatg ttttttgact gtttggatga aagaatatcg
     4921 ggtcataacc aaatccttct tcgccacgcg cttcgtttat tatagtgcct ttaacttcgc
     4981 caataaacac ttgcgggaaa ctgtcaaggc cgacaagggc aatagcacat ataaactttg
     5041 cgcttttatc actgtggcct tcaagcattt taataattgc agcgtttttc tcagtataag
     5101 gtgagccttc cataaaccgc gcagaattaa caccaggaaa accttctaaa gcgttaacac
     5161 ttagtccact atcatcagcg ataacaggta aattcgtcaa tttatgaata gtcatagctt
     5221 ttataaccgc attatcacta aacgattcgc cgtcttcctt tataacgcca gtaaagttaa
     5281 tgtccgccag acttaaaact tcgtaaggcg tattagcaaa aagtgactta aattctttaa
     5341 gtttatttgc gttattcgtg gcaatgacaa tttgtttttt cataatttta taaagtatta
     5401 gtttttaatg ctttttcctt tttatttttt tcaacatctt tccaaataaa atggaataaa
     5461 tagtaaataa gtagcccagt taaactaaag attacaaagt aaatatgcgg gaacactagt
     5521 gcaatgatta gtgtggggac accaccagca atcatatttg ctctaatatc accaagtatc
     5581 ggcatatttc tcctatcaat ccatggtgaa ataaagaaac catcaaaatc ccctggacca
     5641 gggaacttca taccaatata gtggaataaa atattcgtta attgcgctaa aacgatcaaa
     5701 caaaataata ctgtgaccgc gccaagataa tgccgccatt tagtgccaat tttataagca
     5761 tttattaaat agaaagcaac aacaaccatt actccgtgcc aaatcatcgt atgaatcgat
     5821 atagaaactt gtagagtata tacaccagtg gcaagaatca tcacagcaag accaccaata
     5881 agattgaaga ttgaaataaa ggagtaaatg gcttctttaa tcttctcatt tttaataaat
     5941 ggtgtaagtg gatataaaaa gattggtatt gaacaaaatt gtaaggggaa accgtaccaa
     6001 tgataattag caagcccttc atataaattt aagaaaatta gttgtttgta taattcaatg
     6061 ccaaatagta aaacgccaaa ttgaaaaaca gtaaaatcaa tcttatttaa ctgaccaaaa
     6121 acatagcgtt tcgtgttcgc cttgtcggct ttaacaccac ttgaaacaac aatgattgca
     6181 ataaccatgg caaagagcca tccaagatga aacggacccc acgctaagtc atctggattt
     6241 ttcgatggta aaaagttttc accaaaatgc catctttcac caattgcaaa caaaaaaaac
     6301 attggcccta aaatcattaa atattttatt ggtcgctcga tataccaata cttaccagga
     6361 tgataagttt gtaaatatac tttaagcaat agcaacaaac ccattactat aacgaataac
     6421 gctactatac tataacctac taaatttgac atacactctc cttaaggcag attgaagtta
     6481 ttatataact tattgctttt ttattctatt gaaaaaactt tatttgtttg cgtgaagtaa
     6541 taacaatgcg tttttgctgg aaaaaatgat taaataaaaa aagctccctt tttgttgcag
     6601 tagtttttga tgcaacttta ggaagcaatt tcaatctaga tggagc
//
LOCUS       DAOSLP010000047         6344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_11100_length_6344_cov_9.157577, whole genome shotgun sequence.
ACCESSION   DAOSLP010000047 DAOSLP010000000
VERSION     DAOSLP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11100_length_6344_cov_9.157577"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1059
                     /locus_tag="PK460_04450"
     CDS             142..1059
                     /locus_tag="PK460_04450"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOE54358.1"
                     /translation="MDSADAPIKKKSIIKFGTYPQSEVTDPTLLNTLNAASGTLPSSS
                     NSQAWTDYGYYIKGSVSSYMWYIDVITATGKYRGVYFTSYRPYNTTSPSLLFHPLPQF
                     DNGYTTSPVYWFKYEPITWRVLDVQGSSAFLMADLVLDSQDYHYSISDRTIGGSTVYA
                     NNYKESHIRSWLNSNFYNTAFTAAEKARIQTTTVDNSVASTGYSSNTYACANTSDQVF
                     LLSYAEATNASYGLSTDASRQLKSSAYAQSQGVLTDNTNGNSHWWVRSPHSNYASVAR
                     YVNNDGSDINSTRVNYTHHGVVPALWISL"
     gene            1474..2397
                     /locus_tag="PK460_04455"
     CDS             1474..2397
                     /locus_tag="PK460_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE54359.1"
                     /translation="MKYEKISYFVSKWGITERRIRVLCTEGRIEGAVKVGKTWLIPKD
                     ASKPRDRRYKNINDLFFDDINTDAIDNKKAIIDESRPFSQTMIRQLNDKLLVEWTYHS
                     NAIEGNTLTMSETKVVLENGITIKGKPLKDHLEAINHKEAIEYVEELVKKDTKLTEYD
                     IKSIHYLILKEIDNLNAGKYRAQNVLISGSKHIPPSYINVPLEMQMLMEQYEDWAVYH
                     PLIRACLLHGEFVKIHPFVDGNGRTARLLLNFELIKNGYPPVIIKNEDRADYYDALEM
                     ASTTKNYTLFINLVLNLVNEAADSYLLLLGK"
     gene            2465..3010
                     /locus_tag="PK460_04460"
     CDS             2465..3010
                     /locus_tag="PK460_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54360.1"
                     /translation="MWQCNKKFENGKKGNKCSTPHLFEREIKEAFIKAFNLLCQKKNS
                     VLNNCKDFITILNNTKELDEKIMVQETEVKVLINIARNLVEENATTALDQEDYKNRYA
                     KIEKKFKEEQDKLDELLKERERKRVQKNSIKLFMKAYKEMPEILKEWSLEVWITIIDH
                     AIVYSDGKMKFIFKSGDEIKV"
     gene            3753..4232
                     /locus_tag="PK460_04465"
     CDS             3753..4232
                     /locus_tag="PK460_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE54361.1"
                     /translation="MLLNTCATTSPWLRDIGESWNKAGATGDGHAYYLSTIPLIYSPA
                     FYTKKLDWMKKREALLTPFFSNKVVVDGFIRLTNSAVSHDVRDSLSKIDVPTFIISAK
                     QDYLTPIEDQYLLAEKMPNVSHITIENSGHASMYEHPGLFVTLVLGFVNKKAKSYTI"
     gene            4585..5331
                     /locus_tag="PK460_04470"
     CDS             4585..5331
                     /locus_tag="PK460_04470"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOE54362.1"
                     /translation="MKNNFKTVLIKALKIIAIFVGYAVLAALIYLFFYLNGYKSLDDI
                     RGLIDNAGAWAWLIFSIIQIFSTVVLFIIPAQTTAFIVLALALFTPLEAFLLVAINTL
                     IASLLNFSVGKLFGKVIVQKIIGEETIIKYQEKLETKAKIYYPIMMLFPFFPDDEITM
                     LAGLTPMTYRYFIPVTFVTRSVGIATYVFLGSTFNYQNFTVLHWYQFIVIIGLLLYLV
                     FFLADKLEKYLDSTPLSNFSFWRWSTRRKR"
     gene            5480..>6344
                     /locus_tag="PK460_04475"
     CDS             5480..>6344
                     /locus_tag="PK460_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOE54363.1"
                     /translation="MKKFSIVVNSSVDLTEELRKELDLVVAPLKFIMNGQEYINYLDY
                     RNLGFKEFYQKLRDGVVATTSQVNINEYVDIIEKEFKKGNDVLILSLSSALSSNYNSA
                     RLAVDMFKDHPEHKVYLVDTLTASLGEGLVAYAASMKRKEGATLEETYQFIEQFKYNV
                     STWFTVDDLMFLKRGGRLGGVSAIFGTLLAIKPILHVDLEGRLIPMEKTRGRKKALRI
                     IADKVKETYDPSLPNFCYVAHSDDEEAANLLKDMIEGLGIGLKVTLVNYVGPVIGAHV
                     GPGTIGAFFFAK"
BASE COUNT         2148 a         1014 c         1145 g         2037 t
ORIGIN      
        1 atttaaaagc ggttcaactg tgtctgttta gttaaaatta tatcgattat tgatgttttt
       61 ttctacggaa atcacaagtc gtagaattgt ttatgtaaat gaatatggta caattttatt
      121 acaataaagg agatcattga catggattca gccgatgcac ccatcaaaaa aaagtctata
      181 attaaatttg gaacttatcc tcaaagcgag gtaacggacc ccacattatt aaatacgtta
      241 aatgctgcaa gtggaacact gccatcttca tccaatagtc aagcttggac ggattacggt
      301 tactacatca aaggttctgt atcctcttat atgtggtata tcgatgttat aactgcaact
      361 ggaaagtatc gcggtgttta cttcaccagc tatcgaccat ataacacaac gtcaccatca
      421 ctattgtttc acccgttacc acaatttgat aacggctata caacaagtcc tgtctattgg
      481 tttaaatatg aaccgattac ctggcgagtc cttgatgttc aaggcagtag tgccttctta
      541 atggccgatc tcgtcctcga ctcccaagat tatcattatt cgatttccga tcgaaccatc
      601 ggtgggagta ccgtctacgc aaacaactat aaagaatcgc atattcgttc atggctaaat
      661 agtaacttct ataacacagc ctttacagcc gcagaaaagg caagaattca aacaacaaca
      721 gttgataata gcgttgcctc gactggttat agttcaaata cttatgcctg cgctaacacg
      781 agtgatcagg tgttcttact tagttatgct gaagcaacaa acgcgagcta tggattaagc
      841 actgatgcat caagacaact taaatcgagc gcctacgctc agtctcaagg agttttaacc
      901 gataacacaa acggcaattc tcattggtgg gttcggtctc cgcacagcaa ctacgccagc
      961 gtcgcgcgct acgtgaataa cgatggcagc gacatcaact ccacccgtgt caactacacg
     1021 caccacggcg tcgtccccgc tttgtggatt tcactttagg gaaatcataa tcctcgccgc
     1081 aatccaaaat ggccaaagcg tcatcacttt gtgatagcgg aggccatttt aaaggaataa
     1141 ttcatggaaa agaaaagtga actttacgta ttggtaaaag ctaaagcact tgcgagttac
     1201 gtattcacag tgacgaaaaa atcactcaaa aatacaacta tgtgaacgat tactttagaa
     1261 gggttgcacg attgaacgat tacaacctaa tttgtatcaa ttgaaacacc ctttgccatc
     1321 tgcctagtaa agctttgata caattatcga ggcatttttt attatgagta cgcatattta
     1381 ttattccgat atcggtataa tgttgtctta tttctttgaa atgctataat attattgtgc
     1441 cgatatcggt ataataacat tggaggtgat tatatgaaat atgaaaaaat atcgtatttt
     1501 gtctcaaagt ggggtattac agaacggcga atacgcgtgc tttgtaccga aggaagaatt
     1561 gagggcgcag taaaagttgg caaaacatgg ttaattccca aagatgcatc aaaaccacgt
     1621 gatagacgtt ataaaaatat caatgactta ttttttgatg atataaatac tgatgctatt
     1681 gataataaaa aagcaataat agatgaatca cgaccttttt cacaaacgat gatccgacaa
     1741 ttaaatgaca agcttcttgt cgagtggaca tatcactcaa atgcgattga aggcaatact
     1801 ttaacgatgt cagaaacgaa agttgtgcta gaaaacggga ttacaattaa aggcaaacca
     1861 ttaaaagacc atcttgaagc aatcaatcat aaagaagcaa ttgaatatgt tgaagaacta
     1921 gtgaagaaag acactaaact cacagaatac gacataaaat caattcatta tttaatttta
     1981 aaagagattg ataatttaaa tgctggcaaa tatcgcgccc aaaacgtttt aataagtggt
     2041 tcaaaacata ttccgccctc ctatattaat gtgccgcttg aaatgcaaat gttaatggaa
     2101 caatatgaag attgggcagt ttatcatccg cttattcgcg cctgtttact gcacggtgaa
     2161 tttgtgaaaa ttcatccatt tgttgatggt aatgggcgaa ctgcgcgctt acttttaaat
     2221 tttgaactaa ttaaaaatgg ctatccacct gtgattatta aaaatgaaga tcgcgcggat
     2281 tattatgacg ctcttgaaat ggctagtacc acgaaaaatt acactctttt tattaatctt
     2341 gtgcttaatt tagttaatga agccgctgat agttatttgt tattgctcgg taagtaataa
     2401 taagtacatc accgaactca agagttcaaa agtttcgcat tcaacaacta aatataaaag
     2461 agaagtgtgg cagtgtaata agaaatttga aaatggtaaa aaagggaata aatgtagtac
     2521 tcctcattta tttgaaagag agattaaaga agcatttatt aaagccttta acttactttg
     2581 tcaaaagaag aatagcgttt taaataattg taaagacttc atcacaatac ttaataatac
     2641 aaaagaacta gatgaaaaaa taatggttca agagacagaa gttaaagtcc ttattaatat
     2701 cgctcgtaac ttagtggaag aaaacgccac taccgcatta gaccaagaag attataaaaa
     2761 tcgatacgct aaaatcgaga agaagtttaa agaagaacaa gataaacttg atgaattact
     2821 aaaggaaaga gagcgtaaaa gagtgcaaaa gaatagtatt aagttattca tgaaagctta
     2881 taaagaaatg cccgaaatat tgaaagagtg gtctcttgaa gtatggataa caataattga
     2941 ccacgctatt gtatatagtg atggtaagat gaagtttatc tttaaaagtg gtgatgagat
     3001 taaagtataa gtttataaat taaatatgtt tcatatggtt aacaaaacag ggactttaag
     3061 aacttgtcaa gaatgtaatt tatagttagc atttttaaat attaaatagt accaaaccat
     3121 tatttacact ggagagcgaa tctcttttcc ttttttcgga atcctattgt ctgaacgatt
     3181 ataacctagg gggttgcaca tttgaacgat ttgaagggtg gggggtgcat gattgaacga
     3241 ttacttccga aattgtatca attaggacaa catgccatct tactaggtat gccttgatac
     3301 aatttcaagg cattttttat tagaaaaaga tagaaaaata atacattttt tcgataattg
     3361 atagtaattt gacattttaa aggttattga agggagtaat tttaattatc tagaattcca
     3421 ctatctgaac gattagaagt gttgaggcaa gggaagatat tataatagaa aaaattcata
     3481 aatctgcaaa aactggaatc aagcttctga aaaacacgga gcgaaacctc tgaaaaaacc
     3541 ggaataaacc ttttacaaaa catcaaattt aaatatgata aagatgatga ttaatcttta
     3601 aattttcttt tacgccataa taaaaagtca gaaatttgac cttctgactt ttttatttct
     3661 aatacatttt aactagatgc tatttcatta cgatgagtaa ttaatgactt ttatggatat
     3721 cggttaaata ttaagattat gttgaaagtc ttatgttact taatacatgt gcgacaacga
     3781 gcccatggct aagagacatt ggtgaatcgt ggaataaagc aggggcaacg ggagatggcc
     3841 acgcttatta cttatcaacg attccgttaa tttattcacc agctttctac acgaagaaac
     3901 ttgactggat gaaaaaaaga gaagcactct taacaccatt tttcagtaat aaagttgttg
     3961 ttgatggctt tattcgctta acgaatagcg ctgttagtca cgatgtaaga gatagcttaa
     4021 gtaaaatcga tgtaccaaca tttattataa gcgcaaagca agactattta accccaattg
     4081 aagatcaata cttgctcgcc gaaaaaatgc cgaatgtgag ccatatcacg attgaaaatt
     4141 cgggtcacgc tagtatgtat gagcatcctg gtctatttgt gacgctagtc cttggctttg
     4201 tcaacaaaaa agcaaaatca tatactattt aaacactaac gcataaattt atttagcgtt
     4261 actgtttgca aaaacaacca gtaacgtgca aaacttttaa gtataatatt ctaataatta
     4321 aaaggcgcat atttgcctta aagccatata aacctattac gggttaaaat ggcttttttt
     4381 cttgtatttc gcactttgta aaaaagttga taattattca aaagataaaa tgcgattatt
     4441 agcaagatat gtttttatat tattgctttt ctgtttgtta attgtcggga tgtggatttt
     4501 gttataaaca tatttaattt attattaaca tctattttta agtaatgttc acttaaattc
     4561 gttataatgt tggcggtaat agtcatgaaa aataatttta aaacagtttt aattaaagcc
     4621 ttaaagatta ttgctatatt tgttggctat gctgttttag cagctttaat ttatctattt
     4681 ttttacttaa atggttataa aagtttagac gatattcgcg gacttattga taatgcggga
     4741 gcatgggcgt ggttaatctt ttcaattatt caaatctttt caacggtcgt gctttttatt
     4801 attcccgcac aaacgacggc ctttattgtc ttagccttag cgctttttac tccgcttgaa
     4861 gcttttcttt tagtagcgat taatacactt atagcgtcgc tacttaattt ctctgttggt
     4921 aagttatttg gcaaagtaat tgtacagaaa attattggtg aagaaacaat cattaaatat
     4981 caagaaaaac ttgagacaaa agcaaaaatt tattatccaa ttatgatgct ttttccgttt
     5041 tttcctgatg atgaaataac gatgcttgct ggtcttactc cgatgactta tcgctatttt
     5101 atccccgtga cttttgtgac gcgttcagtg ggaattgcga cctatgtttt cttaggtagt
     5161 acttttaatt atcaaaactt tacggtgctc cactggtatc aatttatcgt aattattggg
     5221 ctccttttat atcttgtttt tttccttgcg gataaacttg aaaaatatct tgattccacg
     5281 ccacttagta atttttcttt ttggcggtgg tcaacacgaa gaaaacgcta aatatataca
     5341 cgtattaaaa taagctttta taatttgcgt aatgaacaat tatgaaaaat attcactttt
     5401 gtggactttc aaattttttc tcgtatataa ttagatgttt attaaatatt aatagcgcta
     5461 caaaaggaga aatttattta tgaaaaaatt tagtattgtt gttaactcta gtgttgactt
     5521 aactgaagag ttacggaaag agttagactt agtagttgct ccgcttaagt ttattatgaa
     5581 tggtcaagag tatattaact atttagacta tcgtaattta ggattcaaag aattttatca
     5641 aaagttgcgt gacggtgttg tcgcaacgac atcgcaagtt aacattaatg aatatgttga
     5701 tattattgaa aaagagttta agaaaggtaa tgatgtttta attctttcac tttcaagtgc
     5761 actttcaagc aactataata gtgcacgtct tgctgttgat atgtttaagg atcatcctga
     5821 acataaagtt tatcttgttg atactttaac agcatcactt ggtgaaggct tagtcgctta
     5881 cgctgcttca atgaaacgaa aagaaggagc gacacttgaa gaaacttatc aatttattga
     5941 acaatttaaa tacaatgtct caacgtggtt tacggttgat gatttaatgt ttttaaagcg
     6001 tggtggtcgt ttaggtggag tgtctgctat atttggtaca cttttagcaa ttaaaccaat
     6061 cttacacgtt gaccttgaag ggcgtctcat tccaatggaa aaaacacgcg gtcgtaaaaa
     6121 agctttacgc atcattgccg ataaagttaa agaaacttat gacccaagtc tccctaattt
     6181 ctgttatgtt gcgcatagtg atgacgaaga agcggcgaat ttattaaaag acatgattga
     6241 aggcttagga attggtttaa aggttacttt agtaaattat gttggaccag ttattggggc
     6301 gcatgtcggc ccaggcacga ttggtgcatt cttctttgca aaac
//
LOCUS       DAOSLP010000048         6328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_11141_length_6328_cov_8.494660, whole genome shotgun sequence.
ACCESSION   DAOSLP010000048 DAOSLP010000000
VERSION     DAOSLP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11141_length_6328_cov_8.494660"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1044)
                     /locus_tag="PK460_04480"
     CDS             complement(31..1044)
                     /locus_tag="PK460_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOE54364.1"
                     /translation="MKFNKLISVSILALLISGTSLTTTKQKEKVSAATPMPTNINMTN
                     RTDAEVSAYYEGVNGKSGDALLAFLYTKIKNHNEYNYESVTHRTIYKIIDRNWALDAL
                     DPNGPANTSNFNYSTDNGFIRKLYADYNDNIATADRFKNDGASRVSFDKEHIWAQSLG
                     NFGRTRGAGSDFHSLWPADVKGNQDAHSNYNFAVPKTSITTYKNDYGTYVGRNGYISG
                     YSQKVFEPLDQYKGDIARAMFYMPARYYEYIDALHPKLELVNGSPAAVTASSSVTGKA
                     GDLATLLEWHELDPVDEYEIRRNNLIANNYQGNRNPFIDYPQWARIAYDPTYTGPVFR
                     VIH"
     gene            complement(1165..2664)
                     /locus_tag="PK460_04485"
     CDS             complement(1165..2664)
                     /locus_tag="PK460_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOE54365.1"
                     /translation="MKKRLMTLPLIMLLLSGLKSPISNIEKLNAATPMPTTIVFENNT
                     DAEVSAYYTGVEGKTGDELLEFLYSVIKDHNEYDYENNTHRYIYKIIDRNWELDAIDT
                     VSPANLENFDYVNDNGFIHKLYADYNDDINTADRFKNEGASRVSFDKEHIWPQSLGAF
                     GRTGGAGSDFHSLWPSDVKANQQAHSNNNYGVPTSNITNYQSDHDTYVGRNGNVEGYT
                     SKVFEPLDEYKGDIARAMFYMPARYYEYIDKLHPKLELVNGSPAAREASATLTGQAGC
                     LETLLQWHEEDPVSEYEIRRNNLIANNYQGNRNPFIDYPQWARIAYDEGYIGTGATNA
                     LGLSSAAGLRDPFEDVTLKSISIDTTNVKKEYALNEGYDPTGLVVTAHYENGGKRKVA
                     DFTTSMAKGEPLTKGGTKTITVSVTHEGVTKTASYNIEVKEPLPIYVYIGVAAVVLLI
                     IIIYIFSAKSKKAKKKRASNVKKVIKKATKSSSSTKSNSKRKTTKTKKK"
     gene            2795..4135
                     /locus_tag="PK460_04490"
     CDS             2795..4135
                     /locus_tag="PK460_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOE54366.1"
                     /translation="MNKKEKKLKPGIKKKMSPYLIIVLSFLSVIIIGALLLSLPFAKQ
                     EGVTLSFLDTFFISTSAVTITGLSPIHNLSTTFSVFGKVVIALLTQIGGLGIITLSVF
                     VMILIGMKIGISNRVLLQTNLNSPSMAGVVNLIKKIVIFTLIVEFIGFLINLAVFIPL
                     FPLPKAIGISAFHAIASFNNSGFDILGDSSLINYQDNILLLINTALMIMIGGLGFIAI
                     QDIFTKRSIKKLSIHTKIVIKVNLFLWIVGTLVIKLAQIGGESTSWLTAFFMSVSART
                     AGFAAEKLVNFHSLSLLMLICLMFIGGSPSSTAGGIKTTTFYTLTKSMISYGLGKPTT
                     TYNRVIDQESKNKAFLLLFTALTGVVIGTALILFFDNLTIGRALFETVSALSNVGLSV
                     GTTATLSSASKVVLIVLMFAGRVGPLTIMSLMNRDWYKIGAHPVDYLEEKIIIG"
     gene            4145..4807
                     /locus_tag="PK460_04495"
     CDS             4145..4807
                     /locus_tag="PK460_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOE54367.1"
                     /translation="MKSIAIIGLSRFGLSLVEAFSKLDVELVAIDMDEEQVRKAGDFI
                     QNVFIADSTSIESLRAAGIANVDHAIVAIGQNERINLTTSIISVIKLKELGIKEITAR
                     ADEEDYAEVLTLVGATNIVMPLDIAAERIANKIAAGNVLDYFNVRRDYDVYEVKISDD
                     FEPLPIINLDSRTRYKINILLIERNKVLYVPTKDTILEPGDEIFVFGKKQDIPKVLSV
                     FN"
     gene            4905..6128
                     /gene="ugpC"
                     /locus_tag="PK460_04500"
     CDS             4905..6128
                     /gene="ugpC"
                     /locus_tag="PK460_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOE54368.1"
                     /translation="MAKVKLSGIYKRYEGNKNFSVSDFNLEIGEKEFIVFVGPSGCGK
                     STTLRMIAGLEDITDGELYIDGVLVNDLPPKDRGIAMVFQSYALYPHMTVFDNMAFGL
                     KVQRLPKDEIKRRVHEAARILEIEHYLQRKPRALSGGERQRVALGRAIVRQTEVVLMD
                     EPLSNLDAKLRVQMRSEIIRLHETLGSTTIYVTHDQTEAMTMASRIVVMKDGFIQQVG
                     SPIEIYHNPANLFVASFIGSPPMNFFNVVVQKDHLEFVHYEEREEETEDGEDKVELVS
                     VKERVVDGKLPIPASLAKKLKGYVGKEVVFGVRPENVNYATSEGGREYANFKQNFTVE
                     IAELLGNEYIVHSKLFGQKIISKLLVDPANAIHIGAKLDLVIDFDKVAFFKVDTEAEA
                     AELPSAELNKRIELK"
BASE COUNT         1975 a         1130 c         1114 g         2109 t
ORIGIN      
        1 ttaattatta tttttatccc ctaaactttt ctagtgtata accctaaaga cgggaccggt
       61 ataagttggg tcataagcaa tccgtgccca ctgcgggtaa tcaataaagg gattgcgatt
      121 gccttgatag ttattcgcaa ttaagttatt tcttctaatt tcatattcat caactggatc
      181 aagttcatgc cattccagta gagtcgctaa atcgcctgct ttaccagtaa cactcgaact
      241 agcggtcact gctgctggtg aaccattcac taattctaat ttcggatgaa gtgcgtcaat
      301 atattcataa taacgcgcgg gcatataaaa catagcacga gcgatatcac ccttatattg
      361 atctaacggt tcaaatactt tttgcgagta accacttata taaccattac gaccgacata
      421 agtgccgtag tcattcttat aagttgtaat acttgttttt ggaaccgcaa aattatagtt
      481 gctatgtgca tcttgattac ctttaacatc agcaggccat aatgaatgaa aatcactacc
      541 tgctccacga gtgcgaccaa agttaccaag tgattgcgcc caaatatgct ctttatcaaa
      601 actaacacga ctcgctccgt cgtttttgaa ccgatccgca gtagcaatat tatcattata
      661 atcagcgtat aacttacgaa taaagccgtt atcagtacta tagttaaaat tagatgtatt
      721 cgcagggccg tttggatcaa gtgcatctaa agcccaattc cgatcaataa ttttataaat
      781 tgttctatgc gttacacttt cataattgta ttcattatga tttttaattt tcgtataaag
      841 aaaggctaaa agtgcatcac cacttttacc gttcacccct tcgtaataag cactaacttc
      901 ggcgtcagtc ctattagtca tattaatatt cgtcggcatc ggggtagccg ctgaaacttt
      961 ttctttttgt ttagttgtag taagcgaagt tccgcttatt aatagtgcaa ggattgaaac
     1021 actaattaat ttattgaatt tcataataac tatctacctt atgttgtaat aatgattata
     1081 gcactaacat taaaaaagtc cataaataat aaaaaaggcg tgttttgtcg taaaatacgc
     1141 ctaatttaag ataaatgtta ttagttattt tttctttgtt tttgttgttt ttcttttact
     1201 attactcttc gttgaagagc ttgatttggt cgctttttta ataacttttt taacattaga
     1261 agcacgcttt ttctttgctt ttttagactt cgcgctaaag atataaatga taattattaa
     1321 aagaactact gctgctacac cgatataaac atagattggt agtggttctt tgacttcaat
     1381 attataactt gcagtctttg ttaccccttc atgagtgaca ctaacagtta tggtttttgt
     1441 tccacctttg gttaaaggtt cgcctttagc catactagta gtgaaatctg caacttttcg
     1501 tttgccacca ttttcataat gagcggttac aactagtcct gttgggtcat aaccttcatt
     1561 taatgcatat tcttttttaa catttgtcgt atcaattgaa atactcttta aggtaacatc
     1621 ttcaaaagga tcgcgtaaac ccgcagcact agaaagtcct aatgcgtttg tcgctcctgt
     1681 gccaatgtaa ccttcatcat aagcaatccg tgcccattgc gggtaatcaa taaacggatt
     1741 tctgttacct tgatagttat tagcgattaa gttgtttctt ctaatttcat attcactaac
     1801 aggatcttct tcgtgccatt gcagtaatgt ttcaagacaa ccagcttggc cggttagtgt
     1861 cgcactagct tctcttgcag caggagaacc gtttactagt tctagttttg gatgtagttt
     1921 atcaatatat tcataataac gcgctggcat ataaaacata gcgcgagcaa tatcaccttt
     1981 atattcatca agtggttcaa acacttttga tgtataacct tcaacatttc cgttgcggcc
     2041 aacataagta tcgtggtcac tttgataatt agtaatatta gaggttggaa ccccataatt
     2101 attattacta tgcgcttgtt gattagcctt aacatcactt ggccatagtg agtggaagtc
     2161 acttcccgcc ccaccagtgc gaccaaaggc acctaaagat tgcggccaaa tatgttcttt
     2221 atcaaaacta acacgactcg caccttcatt tttaaagcga tcagcagtat taatatcatc
     2281 attatagtca gcgtataatt tatgaatgaa tccattgtca ttgacatagt caaaattttc
     2341 taaattcgct ggtgatacgg tatcaattgc atctaattcc caattacgat caatgatttt
     2401 ataaatatat cgatgcgtat tattctcata gtcatactca ttatgatctt taataacaga
     2461 gtagagaaat tcaagtaatt cgtctcccgt ttttccttca acaccagtgt aatatgcact
     2521 tacttccgcg tcagtgttat tttcgaaaac aatcgtcgtc ggcatcggtg ttgctgcatt
     2581 aagtttttca atgtttgata tcggggattt tagaccactt agtaataaca taataagtgg
     2641 caaggtcatt aatctttttt tcatcattaa ctccaatctt cttattaaat taacatttaa
     2701 attataccat ttatccttaa gtaacaaaga agggcgatat tttatttagt gttatcttta
     2761 acactttcat gaataaaagc ataaaatata aaatatgaat aaaaaagaaa agaaattaaa
     2821 accaggtatt aaaaagaaaa tgtcgccata tctaattatt gtgctttctt ttttaagtgt
     2881 aattattatt ggtgccttgt tactttcatt accgtttgcg aagcaagaag gagtaacact
     2941 ctcatttttg gatactttct ttatttctac ttctgcggtt acaataacag gtttgtcacc
     3001 cattcataat ttatccacta cattttcagt atttggtaaa gttgtaattg cactattaac
     3061 acaaataggt gggcttggca tcatcacttt aagtgtgttt gtgatgattt taattgggat
     3121 gaaaattggg atttctaatc gtgttctttt acaaacaaat cttaactcac caagtatggc
     3181 gggtgttgtt aatctaatta agaaaattgt catatttaca ctcattgttg aatttatcgg
     3241 ttttcttatt aatctagccg tttttatccc gttatttcct ttgccaaaag cgattggtat
     3301 aagtgctttt cacgccatag cgtcatttaa caattcaggt tttgacatat taggggatag
     3361 ctctttaata aattatcaag ataatattct cttactaatc aatactgcac tgatgattat
     3421 gattggtggt cttggcttta ttgctatcca agatatattt acaaaacgtt caattaagaa
     3481 attaagcatc catacaaaaa tagtaattaa agttaacctt ttcttatgga tagttggtac
     3541 tttagtcatc aaacttgcgc aaattggcgg tgaatcgaca agttggctta ctgctttctt
     3601 tatgagcgtg agcgcgagaa ccgctggttt tgctgctgaa aaattagtaa atttccatag
     3661 tttaagttta ttaatgctta tttgtttaat gtttatcggc ggttcaccta gttccactgc
     3721 tgggggtata aagacaacaa ctttttatac tcttacaaaa agtatgatta gctacggact
     3781 tgggaaacct actacaactt ataaccgtgt tattgaccaa gaatcaaaaa ataaggcgtt
     3841 tttgctgctg tttaccgcat taaccggcgt tgtaataggg accgcgctaa tcttattctt
     3901 tgataattta actattggac gagctctttt tgaaactgta tctgcattgt ctaatgtggg
     3961 cttatcagtc ggaactacgg cgacactaag cagcgctagc aaagtcgttt taattgtact
     4021 tatgtttgca ggaagggttg gtccgctaac aattatgtca ctgatgaatc gcgactggta
     4081 taaaattgga gcgcatccag tagattattt agaagaaaaa ataattatag gttaaaatat
     4141 atttatgaaa agtattgcaa ttattggttt aagtagattt ggcttaagtt tagtagaagc
     4201 ttttagcaag ttagatgttg aattagtcgc tattgatatg gacgaagagc aagtccgtaa
     4261 ggccggcgac tttattcaaa atgtttttat tgctgattcc actagtattg agtctttaag
     4321 agctgctggt atcgctaatg ttgatcatgc aattgtcgcg ataggacaaa atgaaagaat
     4381 caacttaact actagcatta tttcagtcat caaattaaaa gaattaggca ttaaagaaat
     4441 taccgctcgt gctgacgaag aagattacgc agaggtttta acattagttg gtgcgactaa
     4501 tatcgttatg ccacttgata tcgcagcgga aagaatcgct aataaaatag cagccggtaa
     4561 tgttcttgat tatttcaatg tccggcgcga ttacgatgtt tacgaagtta aaataagtga
     4621 tgactttgaa ccacttccaa ttatcaacct tgactcacgt acccgctata aaataaatat
     4681 attgttaatc gagcgcaata aagtgctcta tgttccaaca aaagatacga tattagaacc
     4741 aggtgacgaa atttttgttt ttgggaaaaa acaagacatt ccaaaggttc ttagtgtctt
     4801 taattaatct tcttcttggt tatgaaaatt ttaaatataa catcgattgg cacaaattta
     4861 aaaatgtgtg ctatgattaa attaattgaa gaaggagttt tattatggcc aaagtaaaat
     4921 tatcaggcat ttacaaacgt tacgaaggta acaaaaactt ttcggttagt gattttaacc
     4981 tagaaatcgg tgaaaaagaa tttattgttt ttgttggtcc atcaggctgt ggtaagtcca
     5041 caactttacg gatgattgct gggttagaag atattaccga tggggaactt tacattgacg
     5101 gagtgctcgt taatgactta cccccgaaag accgcggaat tgcaatggtt ttccaaagct
     5161 atgcacttta tccgcatatg actgtgtttg acaacatggc atttggacta aaagtccaac
     5221 gtctcccaaa agatgaaatc aaacgccgtg ttcatgaagc agcaaggatt cttgaaatcg
     5281 aacattatct ccaaagaaaa ccacgtgcgc tctctggtgg tgaaagacaa cgggttgctt
     5341 taggacgtgc aattgttcgt caaactgaag ttgtattaat ggacgaacct ttatcaaact
     5401 tagacgcgaa acttcgtgtg caaatgcgtt cagaaattat tagacttcac gaaacattag
     5461 gtagtacaac aatttatgtt actcacgacc aaaccgaagc aatgacaatg gcctcacgta
     5521 tcgttgttat gaaagacgga ttcatccaac aagttggttc accaattgaa atttatcata
     5581 atccagcgaa cttattcgtc gctagtttca ttggttcccc accgatgaac ttctttaatg
     5641 ttgttgtgca aaaagaccat cttgaattcg ttcattatga agaacgcgaa gaagaaactg
     5701 aagatggcga agataaagtc gaactcgtca gtgttaaaga acgtgtcgtt gatggtaaat
     5761 taccaattcc tgctagttta gcgaaaaaac ttaagggtta tgttgggaag gaagtcgtct
     5821 ttggcgtccg tccagaaaat gtcaactatg ccacaagcga aggtggccgc gaatacgcta
     5881 actttaaaca aaacttcact gttgaaatcg ctgaattact tggtaacgaa tatatcgttc
     5941 actccaaatt attcggtcaa aaaattattt cgaaattact cgtcgatccc gctaatgcaa
     6001 tccacattgg ggcaaaactt gacttagtaa ttgactttga caaagtcgct ttcttcaaag
     6061 ttgataccga agcagaagct gccgaattac cgagcgctga attaaataaa cgtatcgaac
     6121 tcaagtaata atcaacaaaa ttcgagaaaa agctccgcta acaggagctt tttttgatta
     6181 agatcaaaag gtttccaaat tatatacata tatcatttgt caaaatataa aataattact
     6241 ataatgacta tgtctaaact taaatattgg cagagcttaa ctatcgttag catttttagg
     6301 ctctcccaca aaacaatgag aaaggaga
//
LOCUS       DAOSLP010000049         6065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_11771_length_6065_cov_7.587022, whole genome shotgun sequence.
ACCESSION   DAOSLP010000049 DAOSLP010000000
VERSION     DAOSLP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11771_length_6065_cov_7.587022"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..446
                     /locus_tag="PK460_04505"
     CDS             114..446
                     /locus_tag="PK460_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54369.1"
                     /translation="MEQNFRARREKIFGRFKKEQVLRWMMSFTVTTSVAVTAVVGAST
                     AEPEVSFTSVEVLGNTIYYETLVNDPDLTLESESLVLDVKSGLETFSFPLSLGITTGN
                     FNIRYFNI"
     gene            540..1466
                     /locus_tag="PK460_04510"
     CDS             540..1466
                     /locus_tag="PK460_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54370.1"
                     /translation="MATHYTEERQAFTFTFDIFIFNPDLELLDITLFPSFAFSEPGEE
                     GVVTEIIELPRINLEEGTNVCTLESIPTHASFVSLKIAGTKNDEEVILLDESIPLPST
                     FSSYIHAVGFSATSLSLVAEVFAPLLFEGTATLNLYEEETLIAAREFNVPYVDAEGEY
                     IPPIFDFENLSVNTMYTAQIEIPYMNHIRKQNEVYKSELQNVYTAPYYSYNAEHILYE
                     NEMVYITLDVVDEGSIISGAYAECFARDEYGELVPMYINSFENVSATAGESNQYECMV
                     FYTEQNIVEIRVYINKSYNYQYENELIYIVTI"
     gene            1481..2446
                     /locus_tag="PK460_04515"
     CDS             1481..2446
                     /locus_tag="PK460_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOE54371.1"
                     /translation="MSKKFKFNKGLIPKLIVIGIFLALVIIAIISDKVLTGTAFGDFV
                     NNTIGKFFNIIDILKNHWITIAESIVIIVFFWLLNKLFEYILLPLGKKRNKHSTWWVL
                     FRSIFKYLSSAVAIFLVLSAWGVDTPTLLIGAGIVGLAISFGAQSLIEDVIAGLFIIF
                     EKQFKIGDVIQVGETRGYVIDIGIRTTSIEDVYGDILIVNNSDLRMILNTSANLSPAI
                     SEIYISYDQPLENVENIVKKALPTLKERIPDIIEGPSYRGVQKLDSNGVLIRVYAKVE
                     DLKKFQVVRDMNRELKIVFEKNNIKFGFPTVNVNEREEEVKKAKD"
     gene            2511..3287
                     /locus_tag="PK460_04520"
     CDS             2511..3287
                     /locus_tag="PK460_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54372.1"
                     /translation="MENNTSQKISFKFDLYTIIIGALLGLSFVMSLVHNIIRQMGFIA
                     FEFWLLRYAFLALLFCIFLVARALKHKELMLITTTLIVAHYLIFPVGTVSFNGFLDFE
                     IQAQFIVALVLIALILLIVASFIKVDIYEKVETFYKYVVAVLFALIGFVFLLDTFING
                     FRDYGDFRWTRAFFALSGVFYYAALVGLFALPILDTGILTPKKKETVQEVEVEVKSEE
                     EKPKEAEKVEAAPKKTEVKEPKVKVEKETEKAEPEAEPKE"
     gene            3492..4613
                     /locus_tag="PK460_04525"
     CDS             3492..4613
                     /locus_tag="PK460_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOE54373.1"
                     /translation="MNQDFIYGADISSLLAVEELGGTFTSDSGEKQELFEILKSNGFN
                     LARIRLFVDPKSNKGVSYGAGHNDLETALTLAKRAKDHDFKVLLNLHYSDFWADENHQ
                     TLPKAWAKLDFADLEYQVYAYTKEVVTTFKEENITLDYIQVGNEVNKGILWPHGKLFR
                     RKKATQTEYDALAILLNAGIKAIREVTPNVKIMIHLGQGSNPEMYADFLHFMEPRLFK
                     FDAIGLSYYPYIHRQITELQATLLFIKTYYPYERFIVETSYPFENGDETSIVNKDSFP
                     PKRGFLAYTPLGQERVLETILDAAVEEQITGLCYFEPGWINVNGLTVATIEGIKYLKE
                     KNIKAGSTLFNEALFSRSGRPNPALSVKNNMDAFKKSDN"
     gene            4751..5647
                     /locus_tag="PK460_04530"
     CDS             4751..5647
                     /locus_tag="PK460_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE54374.1"
                     /translation="MDIILQTNNLSKRFKNKLALKNVNMTIYRGDIYGFVGENGAGKS
                     TLIRLVTNILRPSTGSYSLNLPKRLGVVGAIVESPAFHPGLNALQNLRYQSDLLGYKK
                     TDEELLVLLDKVGLEEQRNSKKVVKNFSLGMKQRLSIAVTLLSNPEFILLDEPMNGLD
                     PAGIKEMRDLILELNRKGTTFLISSHILTELDKVATRYGFISHGELVEEISVRDLHKK
                     AKSYTEVKLGSEVNAKVRELLSGLNFKEASATTLHFATVAEAQNALKLLVANDISVTS
                     FEEIHETIEDYYLQKINKGVGA"
     gene            5650..>6065
                     /locus_tag="PK460_04535"
     CDS             5650..>6065
                     /locus_tag="PK460_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54375.1"
                     /translation="MKKLLIADLRRIFKGKGIYVLMILGFIFPAISALIVQLSGLAND
                     VAELGDLAAEVFNPVALYAGSFNPTQNIGLILLIVVGVLISGEFTNNTIRNKIVAGHS
                     KTSLFLSSLVTVLTYVFVVVLVNSSFTYLFNSISFG"
BASE COUNT         1989 a          891 c         1198 g         1987 t
ORIGIN      
        1 taaacattat aatgaagaca agaaggatgt aaatttaacc ttttccttta acgaagcatt
       61 aatatttgct tgtaatttta tttattccag tagtaaaatg tttttaagca tatatggaac
      121 aaaatttccg cgcccgtcgc gaaaagattt ttggacgatt taaaaaagaa caagttcttc
      181 gctggatgat gtcttttaca gtgacgacta gtgtcgcggt tactgcggta gtcggtgcca
      241 gtaccgctga accagaagta tcttttacta gtgttgaagt gcttggtaat acgatatatt
      301 acgaaacact cgttaatgat cccgatttga cacttgaaag tgagtcactt gtacttgatg
      361 taaagtctgg gttagaaact ttctcgttcc cgctttcttt aggaataaca acgggcaact
      421 ttaatatccg ttattttaat atttaatatg aactagccat taaaggtcca caaggctacg
      481 gtaaaaagac ttttgcaact tataacatta ttagtgttag tgaaccggca gcgcgcatca
      541 tggccactca ttacactgaa gagcgtcaag cctttacttt tacctttgat atatttattt
      601 ttaatcctga tcttgaatta ttggatataa cgctattccc gtcttttgct tttagtgaac
      661 caggtgaaga aggagtagtg acagagatta ttgaattacc acggattaat ttagaagaag
      721 gcacgaatgt ttgcacactt gaatcaatac cgacgcacgc tagttttgtt agtcttaaaa
      781 tagcgggcac taaaaatgac gaagaagtta ttttattaga tgaaagtata cctttacctt
      841 caacattttc ttcttatatt cacgccgtgg ggtttagtgc gacttcactt tcactcgtcg
      901 ctgaagtttt tgcgccacta ctttttgaag gaacagcaac actaaatctt tatgaagaag
      961 aaacacttat tgcagcacgc gagtttaatg taccttatgt tgatgctgaa ggagaatata
     1021 ttccaccgat ctttgatttt gaaaatttaa gcgtcaatac aatgtataca gcgcagattg
     1081 aaattccgta tatgaaccat attaggaaac aaaatgaagt atataagagt gaattacaga
     1141 atgtgtatac agcgccgtat tactcttata acgcagagca tattttgtac gaaaatgaaa
     1201 tggtatatat cacacttgat gttgttgatg aaggaagcat cattagcggc gcttatgctg
     1261 aatgctttgc acgcgacgaa tacggcgaat tagtgccaat gtacattaat agtttcgaga
     1321 acgtgtcagc tactgcgggt gagagtaatc aatacgaatg tatggtattt tacactgaac
     1381 aaaatattgt tgaaattaga gtgtatataa ataagtcata taactatcaa tatgaaaacg
     1441 aattaattta tatagttacc atttaaagga gggaacgatt atgagtaaga agtttaaatt
     1501 taataaagga ctaatcccga agctaattgt tattggcatc tttttagcgc tagtgattat
     1561 tgcaattatt agtgataagg ttctaacagg tacagcgttt ggtgactttg ttaataacac
     1621 gattgggaag ttctttaaca ttattgacat cttgaagaat cactggatta ccattgcgga
     1681 aagtattgtg attattgtgt ttttctggct cttaaataaa ctctttgagt atattttatt
     1741 gccactagga aagaaacgta ataaacactc aacatggtgg gtattatttc gcagtatctt
     1801 taagtatctt agtagtgcag tagcgatttt ccttgtctta agtgcgtggg gtgttgatac
     1861 cccaacactc ttaatcgggg ccggaattgt cgggttagcg attagttttg gtgcgcagag
     1921 cttaatcgaa gacgtgattg ctgggttatt tatcatcttt gaaaaacagt ttaagatcgg
     1981 tgacgtaatt caggttggag aaacaagagg atacgttatt gatatcggaa ttagaacaac
     2041 ttcaattgaa gatgtctatg gtgatatttt aattgtgaat aacagtgact tacggatgat
     2101 cttaaataca agcgcaaact tatcacccgc aattagtgaa atttatataa gttacgatca
     2161 acctttagaa aatgttgaaa atatagtaaa gaaagcttta ccaacactta aagaaagaat
     2221 tcctgacatt attgaaggac caagttaccg cggtgtgcaa aagcttgata gtaatggggt
     2281 tttaatccgt gtgtatgcga aagttgaaga cttaaagaag ttccaagtag tgcgtgatat
     2341 gaatcgtgaa ttaaaaattg tgttcgagaa aaataatatt aaatttgggt tccccactgt
     2401 taatgttaat gaacgcgaag aagaagtaaa aaaggcaaaa gactaaaaaa gtggtagtta
     2461 tttagttttt tctaaactat aatgaatata aagaaaaggg gaaaaaatct atggaaaata
     2521 atacttcaca aaagatttca tttaaatttg acttatatac gattattatt ggtgcattac
     2581 ttggcttatc atttgtcatg tcacttgtgc ataatatcat aagacaaatg ggctttattg
     2641 cttttgaatt ttggctactg cgttatgctt ttttagcatt actattttgt atctttttag
     2701 tggcaagagc gcttaaacac aaagagttaa tgcttattac aacaacttta attgtggcac
     2761 attatttaat ctttccggtt gggaccgttt cttttaatgg tttccttgac tttgaaatcc
     2821 aagcacaatt tattgtagcg cttgtcctaa ttgctttaat tcttttaatt gtcgcttcct
     2881 ttattaaagt tgatatttat gaaaaagttg aaacatttta taaatatgtg gtcgcggtat
     2941 tattcgcctt aattggcttt gtctttttac ttgatacctt tattaatggc tttagagatt
     3001 atggtgactt tcgctggaca agagcgttct ttgctttaag cggtgtcttt tattacgctg
     3061 ctttagtggg attattcgcg cttccaattc ttgatactgg tattttaacg ccaaagaaaa
     3121 aagaaacagt gcaagaggtt gaagtagaag ttaaaagtga agaagaaaaa ccaaaagaag
     3181 cagaaaaagt agaagctgct ccaaaaaaga cagaagtaaa agaaccgaaa gtaaaggttg
     3241 aaaaagaaac agaaaaagca gaacctgaag cagaaccaaa agagtaatag ttttactttt
     3301 attaaattta tatgctacta taaaaataca ccttgggcgg cactaggcag gaaccaaata
     3361 atagctcaag gtgggcgaca ctacttgtta gtgtcgtttt ttttattact atgttgtgac
     3421 ttttaatgaa agttaagcat tagtcattta taattatagt gttgtattgc ttagttttag
     3481 gagggaagat aatgaatcaa gatttcattt atggtgcgga tatctcttca cttttagcag
     3541 tcgaagagtt aggtggtact tttacgagtg atagtggtga aaagcaagaa ctttttgaaa
     3601 tattaaagag taacggcttt aatttagcgc ggattcgctt atttgtcgat cctaaatcaa
     3661 ataaaggtgt ttcttatggt gcgggtcata atgatttaga gacggcatta acacttgcaa
     3721 aaagagcgaa ggaccatgat tttaaagtat tacttaatct tcattatagt gatttttggg
     3781 cagacgaaaa tcatcaaaca ttacccaaag catgggctaa actagacttt gcggatcttg
     3841 aatatcaagt ctatgcctac actaaagaag ttgttactac ttttaaagaa gaaaatataa
     3901 cacttgatta tattcaagtt ggtaacgaag ttaataaagg aatcttatgg ccgcatggta
     3961 agcttttccg tcgtaaaaag gcaacacaaa cagaatatga cgctttagcg attttactta
     4021 atgctgggat taaagcaatt cgtgaagtaa caccgaatgt gaaaataatg attcatttag
     4081 ggcaaggttc taatccagag atgtacgcag atttcttaca ttttatggaa ccaaggcttt
     4141 ttaaatttga tgctattgga ttatcttatt atccgtatat tcaccgccaa attactgaat
     4201 tacaagcgac acttttattt attaaaactt attatcctta tgaacgcttt atcgttgaaa
     4261 ctagttatcc ttttgaaaat ggtgatgaga caagtattgt aaataaagat agtttcccac
     4321 cgaaaagagg tttcttagcc tacacaccgt taggacaaga gcgtgtcctt gaaacaattt
     4381 tagatgcagc ggttgaggag caaattacag ggttatgtta ttttgaacca ggatggatta
     4441 atgttaatgg tttaactgtg gcaacaatag aaggtattaa atacttaaaa gaaaaaaata
     4501 tcaaggccgg cagtacatta tttaatgaag ctttgttttc aagaagcggc cgacctaatc
     4561 cagcacttag cgttaaaaat aatatggatg cttttaaaaa gagtgataat taaaagggag
     4621 agtcctcgca agtagcgagg gcttttcatt tgtaaagaaa agtttactaa cacgcttacg
     4681 agtttttcgt ttagtgttaa gtagtgtata attacaaagt aattaaaagt gaaagtagag
     4741 aagtgcattt atggatatta tcttacaaac gaataatctc tccaaacggt ttaaaaataa
     4801 actagcgcta aaaaatgtaa atatgacgat atatcgcggt gatatttacg ggtttgttgg
     4861 tgaaaacggc gctggtaaat caacactgat tcgcctagtg acgaatattt tacggccttc
     4921 gactgggagt tattcattaa acttaccaaa aagacttggc gtagtgggag cgattgtcga
     4981 aagtcctgct ttccatcccg gcttaaacgc tttacaaaat ttacgctatc aaagtgattt
     5041 attaggttat aaaaagactg atgaagagtt attagtgtta ctcgataaag ttgggttaga
     5101 agaacaacgc aatagtaaaa aagtcgttaa aaactttagt ttagggatga aacaacggtt
     5161 aagtatcgcc gtgacccttt taagtaatcc tgaatttatt ttgcttgatg aaccaatgaa
     5221 cgggcttgac cccgcgggca ttaaagagat gcgtgactta attttagaat taaatcgaaa
     5281 aggtacgaca tttttaattt ctagtcacat tttaactgag ttagataaag tagcgacgcg
     5341 ttacgggttt attagtcacg gtgaattagt cgaagaaatt tccgtgcgtg acttgcataa
     5401 aaaagctaag agttataccg aagttaaatt aggtagtgag gtaaacgcta aagtgcgtga
     5461 attacttagc ggtttaaatt ttaaagaagc tagcgcgaca actttacact ttgcgactgt
     5521 tgcagaagca caaaacgctt tgaaactttt agtcgctaac gatattagtg ttacttcttt
     5581 tgaagaaatc catgaaacaa ttgaagatta ttacttacaa aaaattaata agggagttgg
     5641 tgcctaatta tgaaaaaatt attaattgct gatttacggc ggatcttcaa aggtaaaggt
     5701 atttatgtct taatgatatt aggttttatt tttccagcga ttagtgcgct tatcgttcag
     5761 ctttcaggtc tcgctaatga tgtggcagaa cttggtgact tagcagcgga agtatttaat
     5821 cctgttgctt tatacgctgg tagttttaat ccaacacaaa acattggttt aattttactc
     5881 attgttgttg gtgttttaat atctggagaa tttacaaata atacaattcg gaacaaaatc
     5941 gttgcaggac actcgaaaac atcgcttttc ttaagttctt tagttactgt tttaacatat
     6001 gtttttgttg tcgtattagt taatagttcc tttacttacc tttttaatag cataagcttt
     6061 ggctt
//
LOCUS       DAOSLP010000050         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_11945_length_5992_cov_6.977261, whole genome shotgun sequence.
ACCESSION   DAOSLP010000050 DAOSLP010000000
VERSION     DAOSLP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11945_length_5992_cov_6.977261"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PK460_04540"
     CDS             complement(<1..914)
                     /locus_tag="PK460_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOE54376.1"
                     /translation="MKTVKFLLTDGQIFGIIALAVLLLFIFIVLIRSIKIVRQATVLV
                     VERLGKYNRICHAGIHFIIPFFERASQPIFLKERVSDFPPQPVITKDNVTMSIDTVVY
                     WQVTDPKLFMYGVISPQSALENLTATTLRNLVGDLELDDTLTSRDTVNHQMWEVLDLA
                     TDPWGIKVTRVELKNILPPRDIQEAMEKQMRAERQKRQEILEAEGKKQAAILIAEGEK
                     EAVILRAEAAKQKMIKEAEGEAAALLAVYEANAKGIDLINKSNPAPGYLTLKGFEALE
                     NLARGQATKIIVPSEIQNVTGLLTSLKEA"
     gene            complement(917..1390)
                     /locus_tag="PK460_04545"
     CDS             complement(917..1390)
                     /locus_tag="PK460_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOE54377.1"
                     /translation="MIQILFLADANWYFGLWLGIFILAIIIEAAEPGLVSIWFAGGAL
                     VAFILSLFPNVPLWVQIIAFIATSGILLLLSFLFFRKSFLNAKSVQTNVDAMLEEEVI
                     LLSSCDKDTLGEVKYRDVIWKVTPKSHDASFEKGETAIVDGIKGNRLVIKKKGTK"
     gene            complement(1458..2057)
                     /locus_tag="PK460_04550"
     CDS             complement(1458..2057)
                     /locus_tag="PK460_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE54378.1"
                     /translation="MENISKVIANNLIFLRRKHNLTQQELAEKINYSDNAVSRWERGE
                     ATPSVETLALIADYFAINVADLLDENFPAENAPKEKGKRVQRIFVILFSVSIVWTFAL
                     IGFIYTQMFKISLGNYGENGWLLFVMSVPISCLVLYFYNKLWGNKVYHLIIFSVFWWS
                     IITTIYLHILVLTGVNLWLIFLLGIPFELAQVLWFFIRR"
     gene            2219..2992
                     /locus_tag="PK460_04555"
     CDS             2219..2992
                     /locus_tag="PK460_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54379.1"
                     /translation="MQKTKTIYYSDPLNEDFGPTPHNLKKITGNYRYNRDNIFIRFFD
                     FLTYRFILTPIAFIYTKLIARIKLANKHLLKDLKKEGVIVYANHTHAQADAFGPPVHF
                     FPKKVTLVTNSANVSLPILGNLTKAWGAFPLPEDYTASKRFVKEFALRLKRKGVVIIY
                     PEAHLWPFYTGIRPFTHNNFAYSVKFNVKSYALTSTYQKNKKGKLKRIVYLDGPFTVD
                     EKLSSKDASVKLRDAIYEVMKKRSKLSTYEKIRYVKRSE"
     gene            2992..3804
                     /locus_tag="PK460_04560"
     CDS             2992..3804
                     /locus_tag="PK460_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE54380.1"
                     /translation="MNIVFAGNYKVFKGMLLGTLSIIKYYKEPLNLYILTMELKEQDP
                     DYIAVSDEDIKLLEKVVKDVNKKSTVTKIDVTGLYIEHLLESPNSKTRYTAYTLLRLL
                     LDEVAGIPLKVLYLDTDVLANDDISLLYNTDISNYELAGVKDRYGRFFMGPRYLNAGV
                     LLLNLKLIKETNLFAKARHYLNKKKVFLSDQTAINKYVRKKLILKRVYNEQKKTKPDT
                     VIRHFSMQFRLFPKFHFVNIKQWDIERLHSVYKCHAFDDILYKYQQIINKEI"
     gene            3806..4747
                     /locus_tag="PK460_04565"
     CDS             3806..4747
                     /locus_tag="PK460_04565"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HOE54381.1"
                     /translation="MKKHEIPVFFACDDNYIPFLGTALHTMIKNASKDYHYKAIVLNA
                     GIKEENKNRIHALENENLTIEFFNISELITKHYENLALRLRDYYSVAIYYRMFIPTLF
                     PQYEKAIYLDADLILVGDISELYNTNIEGKLVGAINDRLVLDVPEFAAYATNAVGVPA
                     HEYFNSGVLVMNLKKFREDDIETKFLHLLETYNFDSVCPDQDYLNVLCRNDKVLLPIG
                     WNKMPLPDPEFDYATLKLLHYNSFEKPWHHDGVLFSKEFWDAALTSPFYEDLLKIKAS
                     VDEEHLKKEEKGVAGLFAKAIYITHEEEVTFKKILAL"
     gene            4752..5537
                     /locus_tag="PK460_04570"
     CDS             4752..5537
                     /locus_tag="PK460_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOE54382.1"
                     /translation="MEDIFKDIRPIVEANIKAAEEAGTLNAKVMPFDHVVTKEERKLV
                     VFKYDIGKKRVKSKIDRYFANILINKKTKEIGKYIKISGIENALDLKGQSFIITANHF
                     SPYDSLIVRYLTNKIGYKNKLSIVINETNLFMEGSLGKIIRSIDVLPYAHDLEYLGKI
                     FNPQLPKVIEKGNIILIYPEQEMWLDYPYARPLMPGAYHYATKLNIPILPLFVTFKKN
                     EYDETIYTINVGTPLYPNQELSLSDNKEIMMQKDYSFKRSVYS"
     gene            5704..>5992
                     /locus_tag="PK460_04575"
     CDS             5704..>5992
                     /locus_tag="PK460_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54383.1"
                     /translation="MDIFINALLHALFTALYFGGVGIIAILFRYVIKLKGEWYRKALH
                     FFFMGSIFFWLYVFTYWYTSVIVLIIFLIFALIGLTILERYSFYHDLLAERF"
BASE COUNT         2011 a         1030 c         1058 g         1893 t
ORIGIN      
        1 gcttctttta aggaagttaa gaggccagta acgttttgaa tttcacttgg cacgatgatc
       61 ttagtggctt gcccacgggc aagattttct aatgcttcaa agcctttaag tgttaagtag
      121 ccaggcgcag gattagattt attaattaaa tcaatccctt tggcgttagc ttcatagacc
      181 gctagaaggg cagcagcttc accttccgct tccttaatca ttttttgctt tgcggcttca
      241 gcgcgtaaga taacagcttc tttttcccct tccgcgatta aaatcgcggc ttgcttttta
      301 ccctcagctt caagaatttc ttggcgtttt tgccgttccg cgcgcatttg tttttccatt
      361 gcttcttgaa tatctctagg tggaagaatg tttttaagtt caacacgtgt tactttaata
      421 ccccatgggt cagttgcaag gtcaagaact tcccacattt gatgattaac agtgtcacga
      481 cttgtgagtg tatcatctaa ttctaagtcc cccactaagt tacgtaatgt cgtagcggtc
      541 aagttttcta atgcactttg cggagagata actccgtaca taaataattt tggatcagtt
      601 acttgccaat acacaacagt gtcaatactc attgtgacat tgtctttagt aataacaggt
      661 tgcggtggga agtcactcac cctttctttt aagaagattg gttgacttgc tcgttcaaaa
      721 aagggaatga taaagtgaat gccagcgtga caaatacgat tgtatttacc tagtctttca
      781 acgactaaga cagttgcttg gcgtacaatt ttaattgaac gaattagcac aataaaaata
      841 aatagtaata agacagcaag ggcaataatt ccaaaaattt gcccatcagt aagtaagaat
      901 ttaacagttt tcatatttat ttagttcctt tcttttttat aactaagcga ttacctttga
      961 ttccatcaac aattgcggtt tcgccttttt caaaactagc gtcatgactt tttggtgtca
     1021 ctttccaaat aacatcacga tattttactt cgccaagtgt gtctttatcg catgaagata
     1081 gtaagattac ttcttcctca agcatagcat caacattagt ttgcacactt ttagcattta
     1141 aaaatgactt gcggaaaaag agaaaactaa gtagtaataa tattccgctt gtggcaataa
     1201 aggcaattat ttgtacccaa agtgggacat ttgggaataa actaagaata aaagcgacaa
     1261 gcgcgcctcc tgcaaaccaa atactaacta gaccaggttc tgcggcttca ataatgattg
     1321 ctaaaataaa aataccgagc cataaaccaa agtaccaatt tgcgtccgcg aggaagagta
     1381 tctgaatcat agatggtgtc ctcctttagt attattatat catatatatg tgtaaataat
     1441 ctttaaatat tacatattta acgcctaata aaaaaccata gtacttgggc gagttcaaaa
     1501 ggaatcccaa gtaagaatat taaccaaagg tttacccctg ttaatacaag gatatggaga
     1561 taaatagtag taataattga ccaccagaaa acactgaaga tgatgaggtg ataaacttta
     1621 tttccccata gtttattata gaagtagagt acaagacaag aaatggggac actcatgacg
     1681 aataaaagcc acccattttc tccgtagttc ccaagactaa ttttaaacat ctgtgtatag
     1741 ataaaaccaa ttaacgcaaa ggtccacaca attgaaacgc taaaaagaat aacgaatatc
     1801 cgttggactc tttttccttt ttctttcgga gcgttttcag cggggaaatt ttcatccaaa
     1861 agatcagcga cattaatggc aaaataatca gcaattaaag cgagtgtttc gacacttggt
     1921 gttgcttcac cacgttccca gcggctcaca gcattatcac tataatttat tttttcggct
     1981 aattcttgtt gtgttaagtt atgtttgcga cgtaagaaga tgagattatt agcgattact
     2041 ttactaatgt tttccataaa ctatgctcct ttacattcgt aaatatagta gcaattatgg
     2101 gtgtttttgt caaattccca gattttgaga attagattct taaacattga taacgcaaat
     2161 tcaacttttc ccttcctaaa tttccccttc atatttaaca tatgaacggg tgaacgttat
     2221 gcagaaaacg aaaacgatat attacagtga tccccttaat gaggactttg gtcctacgcc
     2281 acataattta aaaaagatta ctggtaacta tcgatataac cgtgataaca ttttcatacg
     2341 gttttttgat tttctcactt atcgtttcat tctaactccc atagcgttca tctatacgaa
     2401 acttatcgct cgtattaaat tagcgaataa acacttacta aaagacttaa aaaaagaagg
     2461 agtgattgtc tacgctaatc atacacatgc acaagctgat gcctttggac caccagtaca
     2521 cttctttcca aaaaaggtaa cactcgtcac gaatagtgca aatgtatccc taccgattct
     2581 tggaaattta acaaaagcgt ggggtgcctt cccgttacca gaagactata cagcgagtaa
     2641 gcgttttgtg aaggagtttg cattaagact caagcgcaaa ggagtggtca ttatttatcc
     2701 agaagcgcat ctttggcctt tctatactgg gattagacca tttacccata acaactttgc
     2761 gtattccgtg aagtttaatg ttaaaagcta tgccttaaca tctacttatc aaaaaaacaa
     2821 aaaaggaaag cttaaacgta ttgtgtatct tgacggaccg tttactgttg atgaaaagtt
     2881 aagtagtaaa gacgcaagtg tgaaactgcg tgacgcgatt tatgaagtga tgaaaaagcg
     2941 aagtaagtta tcaacctatg aaaaaattag atatgtaaag aggagtgaat aatgaatatt
     3001 gtatttgccg gtaattataa agtatttaaa ggaatgctct taggcacttt aagtattatc
     3061 aagtactata aagaaccttt aaatttatat attttaacaa tggagttaaa agaacaagat
     3121 ccagattaca ttgctgttag tgatgaagat attaaattat tagaaaaagt agtgaaagat
     3181 gttaataaaa agtctactgt cacgaagatt gacgtaacag gactttatat tgagcatctc
     3241 cttgaaagtc cgaatagtaa gacgcgctac actgcttata cattgttacg tttattatta
     3301 gatgaagtag cgggtatccc tcttaaagtg ctttatttag acactgatgt tttagcaaac
     3361 gatgatataa gccttcttta taacaccgat attagtaatt acgaactcgc gggtgttaaa
     3421 gatcgttatg gtcgtttctt tatgggaccc cgctatttaa acgctggtgt cttattactt
     3481 aacttgaaac ttattaaaga aacaaactta tttgcgaagg caagacatta cttaaataag
     3541 aagaaagtgt ttttaagtga tcaaaccgcg attaataaat atgttcgtaa aaagcttatt
     3601 ttaaaaagag tgtataacga acaaaaaaag acaaagcccg acactgttat ccggcacttc
     3661 agtatgcagt ttcggctctt tccaaaattc catttcgtta atataaaaca atgggatatt
     3721 gaaagattac attccgttta taaatgtcac gcctttgatg acatcttata taaatatcaa
     3781 caaattataa ataaggagat ttaatatgaa aaaacatgaa ataccagtct tttttgcctg
     3841 tgatgacaac tacattccgt ttctaggcac tgctttacat acaatgatta aaaatgcgag
     3901 taaagattat cattataaag cgattgtctt aaatgcgggc atcaaggaag aaaacaaaaa
     3961 ccgcattcac gcgttagaaa atgaaaattt aaccattgaa ttctttaaca ttagtgaact
     4021 tataaccaaa cattatgaaa acttagcgct ccggcttcgt gattactata gtgtcgctat
     4081 ttattatcgg atgtttattc cgactttatt tccgcagtat gaaaaagcca tctacttaga
     4141 tgcggactta atcttagtgg gcgacattag tgaactctat aacacaaaca ttgaaggtaa
     4201 gcttgtcggg gcgattaatg accgtctcgt ccttgatgtg ccagaatttg ccgcgtacgc
     4261 aactaacgcc gtcggggttc ccgcgcatga atactttaat tctggtgttt tagtaatgaa
     4321 cttaaagaaa tttagagaag atgatattga aacgaagttc ttacacttac ttgaaacgta
     4381 taactttgat agtgtatgcc ctgatcaaga ctatttaaac gtcttatgcc gtaatgataa
     4441 agtgctttta ccaattggct ggaataaaat gccgttacct gatccagagt ttgattatgc
     4501 gaccttaaag ttattacact ataactcctt tgaaaagccg tggcatcatg atggggtctt
     4561 atttagtaaa gagttttggg acgccgcgct tactagccca ttttatgaag acttattaaa
     4621 aattaaagct agtgttgatg aagaacattt aaagaaagaa gaaaaaggag tcgcgggcct
     4681 cttcgctaag gcaatttata taacccacga agaagaagtg acctttaaaa agattttagc
     4741 gctataacat tatggaagac atctttaaag acataagacc gattgttgag gcaaacatta
     4801 aagccgcgga agaagcgggt acattaaacg cgaaagtaat gccctttgat cacgtggtta
     4861 caaaagaaga acgtaaactt gttgtcttta agtatgacat tggcaaaaag cgggtaaaaa
     4921 gtaaaattga ccgctatttt gcgaatattc tcatcaacaa aaaaacgaaa gaaattggta
     4981 aatatattaa gattagtggt attgaaaatg cattagattt aaaagggcaa tcctttatta
     5041 ttactgcaaa tcacttttcg ccctatgact cacttattgt gcgctatctt acaaataaga
     5101 ttggttataa aaataagtta agcattgtta ttaatgaaac caacctattt atggaaggta
     5161 gccttggcaa gattattcga agtattgatg ttttaccgta cgcgcatgac ttagagtatt
     5221 taggaaagat atttaacccg caattaccaa aggttattga aaaaggtaat attattctta
     5281 tttaccctga acaagagatg tggctagatt atccttacgc tcgaccttta atgccgggag
     5341 cctatcatta cgcgacaaag ctaaacattc caatcttacc gttatttgta acctttaaga
     5401 aaaatgaata tgatgaaaca atatatacaa ttaatgtggg aacacctctg tatccaaatc
     5461 aagaactaag ccttagtgac aacaaggaaa taatgatgca aaaagactat tccttcaaaa
     5521 gaagtgtcta ttcgtaaact aactaaaaaa tttaatcaaa agaaattgag taatagaagt
     5581 aatcatatta ttcgtgtttg ccgtgcaatt taccacgcaa ttactaatat aatagtaata
     5641 tattccatta gaaaaacaat ttaataaatg cgggtatagt ataattaatt taagatagac
     5701 attatggata tatttataaa tgctttatta cacgccttat ttactgccct atattttggg
     5761 ggagttggta ttatcgctat attatttcga tatgtcataa aacttaaagg ggagtggtat
     5821 cgtaaagctc tccacttttt ctttatgggg agtatattct tttggttata tgtatttaca
     5881 tattggtata catcagtaat tgtcttaatt atttttctta tattcgctct tattgggtta
     5941 acaatattag aaagatattc tttttatcat gacttactag cggagcgctt ta
//
LOCUS       DAOSLP010000051         5962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_12008_length_5962_cov_9.399695, whole genome shotgun sequence.
ACCESSION   DAOSLP010000051 DAOSLP010000000
VERSION     DAOSLP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12008_length_5962_cov_9.399695"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PK460_04580"
     rRNA            complement(<1..330)
                     /locus_tag="PK460_04580"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            786..1139
                     /locus_tag="PK460_04585"
     CDS             786..1139
                     /locus_tag="PK460_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOE54384.1"
                     /translation="MLKDTNINVRVNSDIKNEAETILNKLGLNLSSAIDLYLAQIIKT
                     RGIPFEVKLPNEENKEKVLQIGQIINSLGGAPTTPELNKIIELYAQNDISYDVAIYAI
                     KEILNKLYCETLLLY"
     gene            complement(1136..1741)
                     /locus_tag="PK460_04590"
     CDS             complement(1136..1741)
                     /locus_tag="PK460_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54385.1"
                     /translation="MKNKKKLFWLILPFTLIMFALLFLRYPIMPTNGTYKVFHSPKKH
                     VENVTTLIHQKKDTIDVEWEDVEVTPLYNYKDNFNYLLVDLYPQGFTILKVIRTSPFS
                     VKASQYAMPSVYGRHYYYSDEDTSPQYTSIYAERDVKNEKLYLLQVKNSHGHGHIPAI
                     KGDNCYINLLTLEEIPLNYYEIDEPYAMYEWWFAYMVAFRF"
     gene            complement(1828..2514)
                     /locus_tag="PK460_04595"
     CDS             complement(1828..2514)
                     /locus_tag="PK460_04595"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOE54386.1"
                     /translation="MCRRINTNKIAYVSLVMLLALGLKASIPKKEFMANDHFIFEINH
                     RDKYATAIELTEAGKEQEVLVVPPSIRGFPVRYIGKPKRFFQPLDINALKLTDKQIKI
                     YLPYSLRGQMWLASVRTVEAILALANLDDFDKVLTINSFPEIKLHYLNGKNKLNTFYM
                     YNFESSLNEGYYFMDYINGSNPYVIPSDPVRKGYTFAGWYYQKECATLWNNEVPKSES
                     ESLTLFAKWI"
     gene            complement(2762..3505)
                     /gene="tpiA"
                     /locus_tag="PK460_04600"
     CDS             complement(2762..3505)
                     /gene="tpiA"
                     /locus_tag="PK460_04600"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOE54387.1"
                     /translation="MRKKLLVGNWKMNKTMAETAVFAHASFELLKNAKAKNIDIGVTP
                     PYLSLQTLLNINPKLIIGAQNVHHLEKGAYTGEISIPMLKELGVSWSLVGHSERRTYF
                     NETNESCNLKVLALLENNMTPLYCVGETLQEFEKGVTKEVIKAQIEVGLKGVKDVKEV
                     VIAYEPVWSIGTGKNASKEIAEDICAYIRELLRAKYGGDADLVRILYGGSVKPNNIKE
                     YMAMPNIDGALVGGASLSVESYAELINNM"
     gene            complement(3507..4724)
                     /locus_tag="PK460_04605"
     CDS             complement(3507..4724)
                     /locus_tag="PK460_04605"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOE54388.1"
                     /translation="MAKIIRDLKLANKTVLIRCDFNVPFKGEVISDDNRIRAALPTIK
                     FALDSGAKVVLFSHLGKIKHKGTEEEVEAAKKKNNMVIVFRRLAEILGEDKVFFSPET
                     SGEEVNKRVALLKPGQVLLLQNTRYEKGEEKNDPVLSEKWASFADVFVMDAFGSAHRA
                     HASTVGVPELLKARGKEVALGFLVEKEVEALGRVVHVAKKDRPYIAVIGGFKVSDKIK
                     VIETLLQKCDKVLIGGAMAYTFQVAQGHNIGISPFEPEQVDFAKKCLDTACEKILLPV
                     DAVATDAFQDWTKKITTTDSNIPDGFEGMDIGPKTQKLFADELSKAKMIFWNGPMGVF
                     EQKDFEAGTRAVCEAVSKLKGAFTVIGGGDSAAAVAQFGYKDYFSHVSTGGGASLEMI
                     ENDGHLPGIDVIG"
     gene            complement(4786..5817)
                     /gene="gap"
                     /locus_tag="PK460_04610"
     CDS             complement(4786..5817)
                     /gene="gap"
                     /locus_tag="PK460_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOE54389.1"
                     /translation="MSIKIAINGFGRIGRLAFRRIAETKGMEVVAINDLTDAENLAYL
                     LKYDSAHGRFHVDDISVTADGTAIVFKGKEIPVYAQPDPTQLNWTKHGVDIVLECTGR
                     FRGKEDASVHLQEGVKAVIISAPADKETPMFVYGVNTDKLTKDMTVISGASCTTNALA
                     PVVKVLDDNFGVERGLMTTVHAYTNDQTILDLPKKKAARRGRAGAMNIVPTSTGAAKA
                     IGQVLPHLNKKLDGGALRVPVIDGSMVDLTVLLKKKVTAEEINAVMKKAAEGELKAIL
                     GYTEDEVVSGDIIGDTHGGLFDATLTKVMEVDGEQLVKVMFWYDNEYGYTCQYIRLAQ
                     EVAKVHGVK"
BASE COUNT         1810 a         1204 c          970 g         1978 t
ORIGIN      
        1 ggccgtgtct cagtcccatt gtggccgatc accctctcag gtcggctacg catccgagcc
       61 ttggtaggcc gttaccctac caactagcta atgcgccgca ggtccatcca tcagcgtagc
      121 aattgctact tttataaaag gaagatgcct tcctcgtaat tattcggtat taactaaagt
      181 ttcctctagg tattcccaac tgaagggcag gttacctacg tgttactcac ccgttcgcca
      241 ctaggttaga cgaatctaac ctcgttcgac ttgcatgtat taggcacgcc gccagcgttc
      301 atcctgagcc aggatcaaac tctcaataaa tatagttatt aattaaattt aatgtgttta
      361 tcctagttca taatattaaa gttcaaaagt attgattcaa taaaaaattg acgttgttta
      421 tttgtacatc tgttcagttt tcaaagatca agagcgcttc gcatcccctt tattacgaag
      481 taatatactc tcggggaata tagcgctttc atataatacc atcattttaa agttgtgtca
      541 acaatatgct cattttttac aacttttttt ctagttatga tggagccgct ccagggcgga
      601 cacttgacat attaaaccac aacttaaagt gtgattcaaa taatatgata tataattttc
      661 actttttgta atttaaacca ttaaagtcgc attttatggg gtttttatat taattttaac
      721 ccttaaaatc agactctgta ttgacaatgt taatacatat tagtaaaata aatttgatag
      781 aaacgatgtt aaaagacaca aatataaatg taagagtgaa tagtgatatt aaaaacgaag
      841 ccgaaacaat tttaaataaa ctagggctta atctttctag cgctattgat ttatatttag
      901 cgcaaattat taaaacgcgc ggcatcccct ttgaagttaa attgcccaac gaagaaaata
      961 aggaaaaagt tttacaaatt ggacaaatta ttaattcatt aggtggcgct cctacaaccc
     1021 cagaacttaa taagattata gaactctatg cgcaaaatga tataagctac gatgtagcga
     1081 tatatgcgat taaagaaata ttaaataaat tatattgtga aacactatta ttgtattaaa
     1141 agcgaaaagc aaccatatat gcaaaccacc attcatacat agcataaggt tcatctattt
     1201 cataataatt aagtggtatt tcttctaaag ttaatagatt gatataacaa ttatcacctt
     1261 taattgcagg aatatgacca tgtccatgcg aatttttaac ttgtaacaaa tataattttt
     1321 catttttaac atcacgttca gcataaatac tagtgtattg tggtgaagta tcttcatcac
     1381 tatagtaata atgacgacca taaacactag gcattgcata ttgactagct ttaacactaa
     1441 acggactagt gcggattact tttaaaatag taaatccttg ggggtacaga tccactaata
     1501 aataattgaa gttatctttg tagttgtaaa gtggtgttac ttcaacatct tcccactcaa
     1561 catcgattgt gtccttcttt tgatggataa gcgttgttac attttcaaca tgtttctttg
     1621 gtgaatgaaa aactttatat gtcccgtttg tcggcataat aggatagcgc agaaacaaaa
     1681 gagcaaacat aattaaagta aacggtaata ttaaccaaaa caatttcttt ttatttttca
     1741 ttattgtttc ctccctgttg taattgtata tttttttctt gaatatcaag tttaccactg
     1801 catctaaatc agcatctctt agtttagtta aatccactta gcaaaaagcg ttaacgattc
     1861 actttctgac ttaggcacct cgttattcca caaagtagcg cattctttct gataatacca
     1921 tcccgcaaaa gtgtatcctt tacgaacagg gtcagaaggt atgacataag gattactacc
     1981 attaatataa tccataaagt aatagccttc atttagggaa gattcaaaat tatacatgta
     2041 gaaagtatta agtttatttt tcccgtttag ataatgtaat ttaatttcag gaaatgaatt
     2101 aatagttaat accttatcaa agtcatctag attagccaaa gcaaggatag cttcgacagt
     2161 cctcactgac gctaaccaca tttgcccacg caaggaatat ggaagataaa tctttatttg
     2221 cttatctgtt aacttcagtg cattaatatc taagggttga aaaaatcgtt ttggttttcc
     2281 tatataccga actggaaaac ctctaatgct tggaggcaca accaaaactt cttgttcttt
     2341 tccggcctcg gttaattcaa ttgccgttgc atatttatct ctgtgattga tttcaaatat
     2401 aaaatggtcg tttgccatga attctttctt gggtatactt gcttttaatc ctagcgcaag
     2461 taacatgact aaacttacgt aagcaatttt attagtatta atccgtctgc acatagtttc
     2521 tcccttacgc taaagaatag cgtcaccatt tgatcgaata atcattgacc actacatata
     2581 aacaagtgac ttaaaaaata taatgtaatt ttatcatgag ttttatatac gctatcctct
     2641 ttttataaat cacattttca tcatctttaa ctactgtata ttatttttcg acctcaaaag
     2701 tcgctattta aaaagggaac cattttcttg gtcccctgta atttgcttta tgtagataac
     2761 actacatatt attaataagt tcagcgtaac tttcaacact aagcgaggca ccgccaacga
     2821 gggcaccatc aatatttggc atcgccatat attctttaat gttgttaggt ttaactgagc
     2881 cgccgtaaag aattcgtact aaatcagcgt caccgccgta tttagcgcgt aataattcac
     2941 ggatataagc gcagatgtct tctgcaattt ctttacttgc atttttacca gtgccaattg
     3001 accacactgg ttcgtaagca atcacgactt ctttaacgtc tttaacacct ttaagcccca
     3061 cttcaatttg tgctttaatt acttcttttg tcacaccttt ttcaaattct tgtaatgttt
     3121 caccaacaca gtatagtggt gtcatattat tttcaagtaa agcgaggact tttaagttac
     3181 agctttcatt tgtttcgtta aagtacgtcc ttctttcact gtgaccgaca agtgaccaac
     3241 taacacctag ttcttttagc attggaatac taatttcacc agtgtaagcc cctttttcaa
     3301 ggtgatgaac attttgcgca ccaataatga gctttggatt aatatttaat aaggtttgca
     3361 gtgataaata aggtggggtg accccaatat caatattttt agctttggca tttttaagta
     3421 attcaaagga agcatgagcg aaaactgctg tttccgccat tgttttattc atcttccagt
     3481 tgccaacaag taatttcttc cgcatattat ccaatcacat caatgcctgg taaatgacca
     3541 tcattttcaa tcatttctag tgatgctccg ccacccgtgc ttacatggct aaagtaatcc
     3601 ttataaccaa attgcgcgac ggcggctgca ctatcaccac caccaatgac agtgaatgct
     3661 ccttttaatt tactcacagc ttcgcacaca gcgcgtgtac cagcttcaaa atctttttgt
     3721 tcaaagacac ccattggtcc gttccagaaa atcattttgg ctttgcttaa ttcatccgca
     3781 aataattttt gtgtttttgg cccaatatcc atcccttcaa aaccgtcagg aatattcgaa
     3841 tccgttgtcg ttattttctt tgtccagtct tggaaggcat cagtggcaac agcgtcaact
     3901 ggtaataaaa tcttctcaca agcagtgtct aaacatttct ttgcaaagtc gacttgttca
     3961 ggttcaaacg gtgaaatccc gatattatga ccttgagcga cttggaaagt gtaagccatt
     4021 gccccaccaa ttaatacttt gtcacatttt tgtaataaag tttcaataac tttaattttg
     4081 tcactcactt taaagccacc aataacggca atataaggcc ggtctttttt agcgacatga
     4141 acaacacggc caagtgcttc gacttctttt tcgactaaaa agccaagcgc aacttcttta
     4201 ccgcgggctt ttaagagttc aggaacaccg acagtcgaag cgtgagcgcg gtgggcacta
     4261 ccaaaagcgt ccatcacgaa gacatcagcg aaacttgccc acttttcact aagaacgggg
     4321 tcatttttct cttcaccttt ttcgtaacgc gtattttgta agagtaagac ttgtcctggt
     4381 ttaagtaaag caacacgctt atttacttct tcgccactag tttcagggct aaagaagact
     4441 ttatcttcac ctaaaatttc cgctagacgg cggaagacaa ttaccatatt atttttcttt
     4501 ttagcggctt caacttcttc ttcggttcct ttatgtttaa ttttaccaag gtgtgagaaa
     4561 agcactactt ttgcgccaga atcaagcgca aacttaattg taggtaaagc agctctaata
     4621 cggttatcat cactgatgac ttcgccttta aatggcacgt taaagtcaca gcgaattaac
     4681 actgttttgt ttgctaattt aagatctcta ataattttag ccatattttt tctccttttt
     4741 tagataaaaa gcgcccgaag gcgctttctt taaatcatta ataaattatt taacgccgtg
     4801 aactttggca acttcttgcg caagacggat gtattggcaa gtatagccat attcattgtc
     4861 ataccagaac attactttaa cgagttgttc accatcgact tccataactt tcgttaaggt
     4921 tgcgtcaaat aaaccaccgt gggtatcacc aatgatgtcg ccactgacga cttcgtcttc
     4981 ggtgtaacca agaatggctt ttaattcacc ttcagcagct tttttcatca cagcgttaat
     5041 ttcttcagca gtaacttttt tctttaagag cactgttaag tcaaccattg aaccatcgat
     5101 tactgggaca cgtaaagcac caccatcaag ctttttgtta agatgtggga gtacttgacc
     5161 aattgcttta gcggcaccgg ttgaagttgg aacgatattc atcgcacctg cccgaccacg
     5221 acgtgcagct tttttctttg gtaagtctaa gattgtttgg tcatttgtat aggcgtgaac
     5281 agtagtcatt aaaccgcgtt cgacaccaaa attgtcatca agcactttca cgactggcgc
     5341 tagtgcgttt gtggtacagc ttgcaccact aatgacggtc atatcttttg ttaatttatc
     5401 ggtattaaca ccgtaaacaa acattggggt ttctttatca gcaggagcac taatgatgac
     5461 ggctttcaca ccttcttgta agtgcacact tgcatcttct ttaccacgga aacgaccagt
     5521 acattctaag acgatatcaa caccgtgttt tgtccaattt aattgggttg gatcaggttg
     5581 agcgtaaact gggatttcct ttcctttaaa aacgattgct gttccatcag cggtcacgct
     5641 aatgtcatca acgtggaaac gaccgtgtgc gctatcatat tttaaaaggt atgctaaatt
     5701 ttcagcatca gttaagtcgt taatcgcgac aacttccatt ccttttgttt cggcaatccg
     5761 acggaatgct aagcgaccaa tccggccaaa tccattaatt gcaattttaa ttgacataat
     5821 tttcctcctt tatttatgcg tttatattat acaacattag ttatctaatg tactaacaaa
     5881 aacatacaaa aaaacgaata ttttcactta tgttagatga catacttatc tttacaaata
     5941 aagattagga aacttttaaa tt
//
LOCUS       DAOSLP010000052         5920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_12142_length_5920_cov_9.991986, whole genome shotgun sequence.
ACCESSION   DAOSLP010000052 DAOSLP010000000
VERSION     DAOSLP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12142_length_5920_cov_9.991986"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..577)
                     /gene="ssrA"
                     /locus_tag="PK460_04615"
     tmRNA           complement(236..577)
                     /gene="ssrA"
                     /locus_tag="PK460_04615"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            690..1874
                     /locus_tag="PK460_04620"
     CDS             690..1874
                     /locus_tag="PK460_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54390.1"
                     /translation="MSRKFKQKPKKVKAEKVKREPDMRKRAYLAMLFNNRAAFDGGRR
                     EPWWVAVLFFIASIVIALVPAMVQVGKTKGSDIFKGPLYHTDVAFTKFVETLEEKDAD
                     LTVVSENDENIFKASPEFVNLVANKAFTLTDGATNEVVPYYSFAQKRIVYTRDENNAV
                     VTNEVDFEYLRVYYTGDIQSSFLLEGKVYSGDAFLALKLLSLKEEDAVGNVTSHLIIG
                     RKALYTRLYNPTAINKPGNPALVFEGRTNSLPVGMNIRDFGKVSKDGVPLAKTDIDYT
                     DKVMENFGHMQDLGYKEVKVRTFWFQTGIYAAIFSIIGLVMGLIIFISTRGKMNPNRY
                     LKFGESLKIGAWLLPAPALITLVLGFILPAQYFQMIFIMTLGMRSVWLTMRTLNPNMP
                     QQ"
     gene            complement(1939..2823)
                     /locus_tag="PK460_04625"
     CDS             complement(1939..2823)
                     /locus_tag="PK460_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOE54391.1"
                     /translation="MIIHAGAIMAIKGIFVTNISHNSPKFRIQVDEFLNKAKLIPNLE
                     LIEVKNQHVLSYLNNNTPDFCLFWDKDIYLAKRIENSGIKVFNNPETIYLCDDKALTA
                     AELAKARVAQPRFFVFPLHYWGNIIEYYEEYKEELLALSFPLVIKERFGSFGDQVYLA
                     HSEEELKKIIKEHGTKGLLAQEFVSGEKGTDYRVNVVGDKVIFTVKRVNDKDFRSNIN
                     QGGTASLVKATSEIEDLAIQATKALKGHFAGVDIMLDAKGKPVVIEVNSNMRTVAVNK
                     VSDIDLTLAILSYIVSNI"
     gene            complement(2789..3166)
                     /locus_tag="PK460_04630"
     CDS             complement(2789..3166)
                     /locus_tag="PK460_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54392.1"
                     /translation="MANNTYNYDNIVPEWNYSEFKHLKRVSNPRTAFARGYLFEEGEF
                     YIEPWFYTQLTRILERFRNEHDEIMDVFFNIARKEKYVLFTRDINEPIFDDENYLLVE
                     IEDIIEGAKLIIDDNSRGSDYGD"
     gene            complement(3181..3657)
                     /gene="rlmH"
                     /locus_tag="PK460_04635"
     CDS             complement(3181..3657)
                     /gene="rlmH"
                     /locus_tag="PK460_04635"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOE54393.1"
                     /translation="MKIDVIVVGKTKGALLITSDEYTKMISKYADISIIEIKEERLPL
                     RASEAQIENALNREAETILKRIGERDFVYALSPEGIEFDSIMFSEQLRNAFDIGQSKV
                     MFVIGSSHGLGNEVYKRANLKLSFSKMTFPHQLFRVMLLEQIFRAFKIINNETYHK"
     gene            complement(3647..4405)
                     /locus_tag="PK460_04640"
     CDS             complement(3647..4405)
                     /locus_tag="PK460_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE54394.1"
                     /translation="MKYFVLASGSKGNATIIKGPETKILIDLGLSLKEYKERLNVFKL
                     QEERVFAVLFTHKHGDHITSQFNKVANGNVYAPANTLPFEAPFKLVVPYTPFTINEFK
                     VTPLPTSHDVSDSVGYIIETTEYKLVYMTDTGYISTRNLEMMKNADAYIIESNHSPSL
                     LLKTNRPFDLIQRILSDTGHLSNEACAHYLAELVGPRTKHIVLAHLSEEANTPEVALE
                     TLDKVFRKYGIDQSKLTIVTAKQHVPIMGELNEN"
     gene            complement(4402..>5920)
                     /gene="dnaB"
                     /locus_tag="PK460_04645"
     CDS             complement(4402..>5920)
                     /gene="dnaB"
                     /locus_tag="PK460_04645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOE54395.1"
                     /translation="VVMKFDNNPFTLSDKTIAIPNDTILERTVLGLMLTSAEALEVGM
                     QHLEASYFPNIDNYNRAIFYAMKLLHEENVKVDIVTVSGKLRELRLFSAVGGLEYLSL
                     IAEEAITYSAIKDYCLGLRDLDLLRKALKVMDQGVEKHRQGQVGDVNDYLSDLLRDIT
                     KVAEERRIMDFESLGDIAKGLKAHLEKMRKSSGNKLTGNSTGFYDIDRYTHGFQEDNY
                     IIIAARPAVGKTAFGLSLAYNMAKINNKTVGFFSLEMGNQQLMQRLVSKVSGVLHKNI
                     MTGYLSKEERVKVYETIENLKHIKLYIDETPSININDLLIKARKLKRENDDLCAIFVD
                     YIGLITTTGHVESRQIELSEISAALKGLARELNITIVALSQLSRAVEATKDKMPQMSH
                     LRESGSLEQDADLVFLMYREDYYVKQGTIKEIDAVYQPYYQTLAQAKAEMKDPSKDLD
                     VSPVDIILAKNRSGETGIATLLFFKAISSFDNPGPDLTARFTEYRTKKGPSYKHE"
BASE COUNT         1792 a         1099 c          997 g         2032 t
ORIGIN      
        1 tatttttaaa actttatttt ctattttgtc actttatctt tttcatgtaa caattaacat
       61 ttacaaaagc tgatttaaca ttctataaat aaaaaatagc gtagttagtt aacttaaaag
      121 caccctaaat tctagatagt ataaagggtg ctttttttaa aaaatagtgg gcaatgaagc
      181 ccacatgatg acgacttggt ttcgatgaca tagaaacctt atcttttttc ttttttggtg
      241 gagatgcgga gagtcgaact ccggtccaag aaccgcccca taaatacttc tacagcttag
      301 aagatattac gatttaactc tgtttgtcga tatctacgaa caacaagaga gcgagcctat
      361 aatacttcat caccgcttat agacatcacg atgactagcc ccttttatga cgcgcgttag
      421 agtgttggag ccccgctcta agttgcgctc ctgcttatct attaagcgaa ggagtatgat
      481 aaattactgt tgccagttaa tttgtggttt ggtgattacg tcaccacacg gctgctatac
      541 ttaccagaca tgtccctgtc gaaaccatgc catccccagt gccactatta tatgacaata
      601 taaaaataat gcaagatgaa aatggactta gttgaaaatt gcttgcaagt aatatatact
      661 tacatggatg taaatcgagg tatatacata tgagtagaaa atttaaacaa aaacctaaaa
      721 aagtaaaagc agaaaaagta aaacgcgaac ctgatatgcg taaacgcgct tacttagcga
      781 tgctttttaa taatcgcgct gcatttgacg gcggaagacg cgaaccgtgg tgggtcgcag
      841 tgctcttttt tatcgcttca atcgttattg ctttagtgcc agcaatggta caagttggta
      901 agactaaggg aagcgacatc tttaaagggc ctttatatca cactgatgta gcgtttacga
      961 agtttgtaga aacacttgaa gaaaaagacg ctgatcttac agttgtaagt gaaaatgatg
     1021 agaatatctt taaggctagt cctgaatttg ttaacctagt agcgaacaaa gcctttactt
     1081 tgacagatgg cgccacgaat gaagttgttc cttattactc cttcgcgcaa aagcgtatag
     1141 tgtatacgcg tgacgaaaat aatgctgtcg ttactaatga agttgatttt gaatacttac
     1201 gcgtttatta cactggtgat attcaaagtt cgtttttact tgaaggaaaa gtttatagtg
     1261 gagatgcttt tttagcgctt aaacttttat cattaaagga agaagatgct gtcggtaatg
     1321 ttacttcaca tttaattatt gggcggaagg cattatacac aagactttat aatccaacgg
     1381 ccattaataa acctggtaac cctgctttag tgtttgaagg acggacaaat tcattaccag
     1441 ttggtatgaa tattcgtgat tttggtaaag tgagtaaaga tggtgtccca cttgccaaaa
     1501 ccgatattga ttacactgat aaagttatgg aaaactttgg tcatatgcaa gacttaggtt
     1561 ataaagaagt taaagtacgg accttctggt ttcaaactgg aatttacgct gctatcttct
     1621 ctattattgg tcttgttatg ggtttaatta tctttatttc tacacgcgga aaaatgaatc
     1681 caaatcgcta tcttaagttt ggtgaatcac ttaaaattgg tgcgtggtta cttcctgctc
     1741 cagctttaat cacactcgtg cttggcttta ttttaccagc gcaatatttc caaatgattt
     1801 tcattatgac gcttggtatg cgcagtgtat ggttaacaat gcggacatta aatccaaata
     1861 tgccgcaaca ataacattaa gaattaataa aaattcacct tgctaaaaac aaggtgattt
     1921 ttttaacttt tatgtacatt aaatattact aacaatataa cttaatatcg ctagtgttaa
     1981 atcaatatca cttactttat taactgcaac cgtgcgcata tttgaattta cttcgatcac
     2041 tactggtttt cctttagcgt caagcataat atcaacaccc gcgaaatgac cttttaatgc
     2101 ttttgttgcc tgaatcgcta aatcttctat ttcgctagtg gcttttacta atgaagcagt
     2161 gccgccttga ttaatattac ttctaaaatc tttgtcattg actcttttaa cagtaaatat
     2221 taccttatct cccacaacat taacacggta atctgtccct ttttcgccgc tgacaaattc
     2281 ttgagccaaa agtccttttg tgccgtgctc ttttataatc ttttttaact cttcttcact
     2341 atgcgctaaa taaacttgat caccaaatga accaaagcgt tctttaatga caaggggaaa
     2401 agataatgca agtaactctt ctttatactc ttcataatat tcaataatat tgccccagta
     2461 atgaagcgga aatacaaaga agcgtggttg cgctacacgc gctttagcaa gctccgcagc
     2521 ggttaatgct ttatcgtcac agagataaat tgtttcagga ttattaaaaa ctttaattcc
     2581 gctattttca atacgttttg ctagatatat atctttgtcc caaaaaagac aaaagtcagg
     2641 tgtattatta tttaaataac ttaagacatg ttgattttta acttcaatta attcaagatt
     2701 tggtataagt ttagctttgt ttaaaaattc atcaacttga atgcgaaatt ttgggctatt
     2761 atgactgata ttcgtgacaa aaatgccctt aatcgccata atcgctcccg cgtgaattat
     2821 catcaataat taattttgca ccttcaatta tatcttcaat ttccaccaaa agatagtttt
     2881 cgtcatcgaa aattggttca ttaatatcac gagtaaataa tacatatttt tctttacgag
     2941 caatattaaa aaatacatcc attatttcat catgttcatt tctaaatcgt tctaagattc
     3001 tcgttaattg tgtataaaac catggttcaa tataaaattc accctcttca aataagtatc
     3061 ctctcgcaaa agcggtccgt gggttactaa cacgctttaa atgtttaaat tcgctatagt
     3121 tccactctgg aacaatattg tcataattgt aagtattatt tgccatatta atatttctcc
     3181 ttatttatga tatgtttcgt tatttataat tttgaatgct ctaaatattt gttcaaggag
     3241 cattactcta aaaagttgat ggggaaacgt cattttgcta aaacttaatt ttaagttcgc
     3301 acgcttatat acttcatttc ctaaaccatg ggaactaccg ataacgaaca ttactttact
     3361 ttgaccaata tcaaaggcgt tacgtaattg ttcgctgaac ataattgaat caaactctat
     3421 tccttcaggt gacaaggcat aaacaaagtc gcgttcacca attcttttta aaatggtttc
     3481 ggcttctcta tttaatgcat tttcaatctg tgcttcactt gcgcgaagcg gaagtcgttc
     3541 ttctttaatt tcaataattg aaatatccgc atatttacta atcattttag tgtattcgtc
     3601 actagtaatt aaaagtgcac cttttgtttt cccgacaaca ataacatcaa ttttcattta
     3661 actctcccat gattggcaca tgttgttttg cggtaacaat tgtcagttta ctttggtcaa
     3721 tcccgtactt ccgaaatacc ttatcaagtg tttcaagcgc aacttcggga gtattagctt
     3781 cttcactaag gtgcgctaaa acgatgtgtt tagtgcgcgg gccaacaagt tctgctaaat
     3841 agtgagcaca agcttcgttt gataagtgac cagtatcgct tagtattcgc tgaattaaat
     3901 caaagggtct atttgtttta agtaataaac tcggactatg atttgattca ataatgtagg
     3961 cgtcagcgtt tttcatcatt tccaagttac gagtgcttat ataaccagtg tcagtcatat
     4021 acacaagttt gtactctgtt gtttcaataa tataaccaac tgaatcactt acatcatgac
     4081 ttgttggtag gggcgttact ttaaattcat taatcgtaaa aggggtgtag ggcacaacta
     4141 gtttaaatgg tgcttcaaac ggcaaagtgt tcgccggagc atacacattc ccgttagcga
     4201 ctttattaaa ttgtgaagta atgtgatcac cgtgtttatg agtaaaaagc acggcaaaaa
     4261 cacgctcttc ttgtaattta aagacattta gtcgttcttt atattcttta aggctgagcc
     4321 ctaaatcaat taatattttt gtttcaggtc ctttgataat tgtggcatta cctttggacc
     4381 cgctagcgag gacgaagtac ttcattcgtg tttataactt ggtccttttt tagtgcgata
     4441 ttcagtaaac cgcgctgtta agtctggtcc tggattatca aaacttgaaa tagccttgaa
     4501 aaatagcagg gtcgcaattc ccgtttcacc acttctattt ttcgctaaga taatgtcaac
     4561 tggtgaaaca tcaagatctt tgctcgggtc tttcatttcc gctttagctt gtgcgagtgt
     4621 ttggtaatag ggttgataaa cagcatcgat ttctttaatc gttccttgtt tgacataata
     4681 atcttcgcga tacattaaaa acactaaatc tgcgtcttgt tctagactac ctgattcacg
     4741 aaggtgactc atttgtggca ttttatcttt tgtggcttca actgcccgtg aaagttgtga
     4801 taaggcaacg attgtaatat ttaattcgcg cgctaagcct ttcagggccg cacttatttc
     4861 gctaagttca atttgcctac tttcaacatg tcccgttgtc gtaattaaac caatatagtc
     4921 aacaaaaatg gcgcataaat catcattttc gcgttttaat ttccgtgctt tgataagtaa
     4981 gtcattaata tttatacttg gtgtttcatc gatatagagt ttgatatgct ttaagttttc
     5041 gattgtttca taaactttta cgcgttcttc tttactaagg taccccgtca taatattttt
     5101 atgaagcaca cctgaaactt tagaaacaag ccgttgcatt agttgttggt tacccatttc
     5161 aagcgagaag aaaccaacag ttttattatt tattttagcc atattatagg ctaagcttaa
     5221 accaaatgcc gttttaccga ccgcaggacg tgcagcaata ataatatagt tatcttcttg
     5281 gaaaccgtga gtgtaacgat ctatatcata aaagcccgtc gaatttcctg tgagtttatt
     5341 accagaagat ttccgcattt tttcaaggtg agcttttaac cctttagcga tatcaccaag
     5401 tgattcaaag tccataatcc gtcgctcttc cgcaacttta gtaatgtcgc gtaataaatc
     5461 acttaagtaa tcattaacat caccaacttg accttgacgg tgtttttcaa cgccttgatc
     5521 cattactttt aaggctttac gaagtaaatc taaatcacgt agtcctaagc aataatcttt
     5581 tatcgctgaa tatgtaatcg cttcttcggc aattaaactt aagtattcta aaccaccaac
     5641 agcgctaaat agtcgtaatt ctcttagttt accgctgacg gtaacaatat caactttgac
     5701 attttcttca tgtaataatt tcattgcata aaaaatagcg cggttataat tatcgatgtt
     5761 tggaaaataa cttgcttcta agtgctgcat ccctacttct agtgcttcag cacttgttag
     5821 cataagacct aacactgttc tttctagaat agtgtcatta ggaatcgcta ttgttttatc
     5881 cgatagtgta aaaggattat tatcaaattt cataactact
//
LOCUS       DAOSLP010000053         5771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_12545_length_5771_cov_8.340973, whole genome shotgun sequence.
ACCESSION   DAOSLP010000053 DAOSLP010000000
VERSION     DAOSLP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12545_length_5771_cov_8.340973"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1073
                     /locus_tag="PK460_04650"
     CDS             69..1073
                     /locus_tag="PK460_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE54396.1"
                     /translation="MSDAYKDRVTIYEVAAAAGVSLATVSRVINNHPNVTPATRAKVQ
                     NAITRLGYRPSALAQALATSKTTNIGLIIPSANYVFISNMLHGIVMRANSLGYLITLF
                     VTGTEKEDALDAMDKLISSHVDGAIIFDDLFEEEEVTQITNYQVPVVVVNNRLDEDEK
                     VACITFGYENTLREDIIRHHLKTSDVMMHFVALNDNGRLLDRLQRSFVNTHKEENREY
                     KIHKVQDGYHAHYAYFKELFKTTKEGFFVAYRDSLGRAIVNAALENGLRIPEDIEVIS
                     LIGTRYAVQGRPKLSSMILDMEEVGNKALEMLIEILEGKTHLPRQKVVAEYIKLDTTR
                     "
     gene            1085..2335
                     /gene="tyrS"
                     /locus_tag="PK460_04655"
     CDS             1085..2335
                     /gene="tyrS"
                     /locus_tag="PK460_04655"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOE54397.1"
                     /translation="MNIFDELEYRGLIKDYSNAANIKEMLNTPQKIYCGFDPSASSMH
                     MGNFVMIMTLMRLQRAGHKIIAVVGGATGMIGDPSGKSAERNLLDKDALRHNTAQIRA
                     QLEKYLDLSDPQKGEIVNNYDWHGNINVLEFLRDYGKYFTVNYMLAKDTVARRLETGI
                     SYAEFSYMLLQAVDFLYLYEHYACTMQIGGSDQWGNLISGLDLIRKVKGADAKVEVMT
                     AHLLTDAEGNKFGKSVDGALFLDAKKFSPYKLYQYFYNVGDADAEKYLKVFTFLSREE
                     IEAITSEHKEAPHRRLAQTRLAEEIVTIVHSKEALLHAQNMSTALFSGNVKDLSAEDI
                     EDLFAHALVGLDSDQKLEDVLITLKAATSKREAREFIRGNSISVNGERLTDPELIITK
                     DYLLHDKYLIIRRGKKNYFVAEVK"
     gene            complement(2402..3010)
                     /gene="rpsD"
                     /locus_tag="PK460_04660"
     CDS             complement(2402..3010)
                     /gene="rpsD"
                     /locus_tag="PK460_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOE54398.1"
                     /translation="MRDTGSVWKKSRRLGYSILETGEELRKRPYGPGQHGNNRRRKVS
                     EYGKQLMEKQKLRHTYGVNERQFKRLFLLAKKNKEVVTGLAFIQILESRLDNLVFRLG
                     LAATRNQARQLVNHGHIEVDGQKVDIPSFLVPVGSTITVRENSRDLKLFKFNLEKVGR
                     TVPFVTLNAEKLSGTFDRMPERTEVAQDIQENLIIEFYNRSM"
     gene            complement(3241..3405)
                     /locus_tag="PK460_04665"
     CDS             complement(3241..3405)
                     /locus_tag="PK460_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOE54399.1"
                     /translation="MSAADIIILIVVIILLGGIIFYLVRRNKKKCYSCSYAKSCETYC
                     HVIKENDPKE"
     gene            complement(3402..5372)
                     /gene="feoB"
                     /locus_tag="PK460_04670"
     CDS             complement(3402..5372)
                     /gene="feoB"
                     /locus_tag="PK460_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOE54400.1"
                     /translation="MKIALIGNQNSGKTSLFNLLTGNNQKVGNWPGVTLERKTGILRT
                     SNAEVIDLPGIYSLSPYSHEENIVRRFLLEEKVDLIINIIDSTAIERSLYLTTQLFNF
                     NIPMVIALNMTDLAFEQGIRIDVNKLSKALSVPVVPISCLKRTGIKDLIMLITKDTPT
                     STNYLTIYSNPLQETINKTAELLPIDAKEFLAIKLLENDIDLAPRIDLDVSKDIKKLN
                     DHYKLGISEVLANERYNFIEKVRDIAVSSREKPKLLTDKIDRILLNKYLAIPIFIIIM
                     LLVYFLAAGPVGIFLSSLVEQGFAALNEGLATFLTNVGASAWSVSLVVDGILTGVTSI
                     LSFVPQLLILFFMIDILEASGYMSRIAFFFDRVFRKVGLSGKALIPFIIGTGCSVPAI
                     MASRVIEDERERKLTVLLTPFIPCGAKLPIITLFAAYFFPDSMGLVSASLYFLSIIII
                     LLTALLLKRILYKQKYSPFIQELPTYKAPNMRYVLRDTGHKTWSFITNAGSVILFASV
                     ILWLLLNFNWKMQFGVAIEKSILSDIGRLFSFVFYPFLGTNSWAAAVSIIQGLIAKEQ
                     VVSSLSIIAKLSGGNTIFESPLFNFFTPASAYAFMAFNLFSAPCFGAIGAMNHEFGNR
                     RTMIKALLLQTIAALVIATLIFQVGTFVEGLL"
     gene            complement(5369..5611)
                     /locus_tag="PK460_04675"
     CDS             complement(5369..5611)
                     /locus_tag="PK460_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOE54401.1"
                     /translation="MSDTLTLDKLTIGQKARVLRINDDNPALKQRLLDMGITTGVIVK
                     IDKISPLGDPFCLELRNYRLLVRRADVSAIEVEVIE"
BASE COUNT         1891 a         1029 c         1091 g         1760 t
ORIGIN      
        1 gctttctctt ctacattgac aatgaaaata ttttcaagta aaattggtat gaaagaaggc
       61 tttttaatat gagtgatgca tataaagaca gagtaacaat ctacgaagta gcagcagcgg
      121 ctggcgttag cctagcaact gtgtcgcgtg tcattaataa tcatccaaat gtcacaccag
      181 cgacgcgggc taaagtccaa aacgccatta cgcgccttgg ttatcgtcca agtgctttag
      241 cgcaagcgct agccacgagt aaaacgacga atattggctt aattattcct agtgccaact
      301 atgtatttat ttcaaatatg ctccacggca tcgtgatgcg tgcgaatagt ttagggtatc
      361 taattacttt atttgtcact ggtacagaaa aagaagatgc acttgacgct atggataaat
      421 taattagttc gcatgttgat ggggcaatta tctttgatga cttatttgaa gaggaagaag
      481 taacgcaaat tacgaattat caagttcctg ttgttgtcgt taataatcgt cttgatgaag
      541 atgaaaaagt tgcatgtatt acttttggtt atgaaaatac attacgtgaa gatattattc
      601 gtcatcacct aaaaacaagt gatgtgatga tgcattttgt agcacttaat gataatggtc
      661 gtttgttaga tcgtttacaa agaagctttg ttaacacaca taaagaagaa aatcgtgaat
      721 ataagattca caaagtacaa gacggatatc acgcgcatta tgcttacttt aaagaactat
      781 ttaagacgac aaaagaaggg ttctttgttg cttatcgtga ctcattagga cgcgcgattg
      841 ttaatgccgc gcttgaaaat ggcttacgca ttccagaaga cattgaagta attagtttaa
      901 ttgggacacg ctatgctgtg caaggccgcc caaaattatc aagtatgatt cttgatatgg
      961 aagaagttgg taataaagca ttagaaatgc taattgaaat attagagggt aagacacact
     1021 taccgcgaca aaaagttgta gccgaataca taaaacttga tacaactcgc tagaaaggtt
     1081 cgttatgaat atatttgatg aattagagta tcgtggttta attaaagatt actctaacgc
     1141 tgctaacatt aaagaaatgt taaatacgcc acaaaaaatc tattgtgggt ttgacccaag
     1201 tgcttcaagt atgcatatgg gaaattttgt gatgattatg actctgatgc gcttacaaag
     1261 agcagggcat aaaattattg ctgttgtcgg tggtgcgacg gggatgattg gtgacccaag
     1321 cggtaaaagc gctgaacgta atttacttga taaagatgct ttacgacata ataccgcgca
     1381 gattcgcgct caattagaga aatatctaga tttaagtgac ccccaaaaag gtgaaattgt
     1441 taataattat gactggcatg gtaacattaa tgttttagaa tttttacgcg attacggcaa
     1501 atattttacc gttaactata tgttagcaaa agacactgtc gcgcgtcgtc ttgaaactgg
     1561 aattagctat gctgaattta gttatatgct cttacaagcc gttgactttt tatatctcta
     1621 tgaacattac gcttgcacta tgcaaattgg tggtagtgac cagtggggca atttaatcag
     1681 cggacttgac cttattcgta aggtaaaagg tgctgatgca aaagttgaag taatgacggc
     1741 acacttatta acagatgcgg aaggtaataa atttggtaag agtgtggacg gcgcactttt
     1801 ccttgacgca aagaaattta gtccatataa gctttatcaa tacttttata atgtcggtga
     1861 cgctgatgct gaaaagtatc ttaaagtatt taccttttta agtagggaag aaattgaagc
     1921 aattactagt gaacataaag aagcaccaca ccgccggctt gcccaaacta gactcgccga
     1981 agaaattgtg acaattgttc atagtaaaga agccttactc catgcccaaa atatgagcac
     2041 tgcccttttt agcgggaatg ttaaagattt aagtgccgaa gacattgaag atttattcgc
     2101 ccatgcacta gtggggcttg acagtgacca aaaactcgaa gatgttctta ttacgctaaa
     2161 agcggcgaca agtaaacgtg aagctcgtga atttattcgt ggtaacagca tcagtgtaaa
     2221 tggcgaacgt cttactgatc cagaattaat tattactaaa gattatttat tacacgataa
     2281 atatttaatt atccgccgtg gtaagaagaa ctactttgta gccgaagtaa agtaaaaagc
     2341 tgcaaattga aaatcaaaaa aactccgcta ttatgcggag tttttgatta agtctgctta
     2401 attacattga acgattgtag aattcaatga ttaagttttc ttggatgtct tgagcaactt
     2461 cagtgcgttc tggcatccgg tcaaatgtac cgcttaactt ttcagcgtta agcgtcacaa
     2521 atggaactgt acgaccaact ttttcaagat tgaacttaaa gagttttaag tcgcgactat
     2581 tttcacggac cgtaatggtg ctgccaactg ggactaagaa gcttgggata tcaacttttt
     2641 gaccgtcgac ttcaatatgg ccgtggttaa cgagttgacg agcttggttg cgggtcgctg
     2701 cgagtccaag acggaacact aagttatcaa ggcgtgattc aagaatttga ataaacgcta
     2761 aaccggtaac gacttcttta ttctttttcg ctaataagaa aagtctttta aattggcgtt
     2821 catttacacc ataagtatgg cgtaattttt gtttttccat taattgttta ccgtattcgc
     2881 tgactttacg acggcggtta ttaccgtgtt gaccaggacc atacggacgt tttcttaact
     2941 cttcaccggt ttctaaaata gaataaccaa gacgacgaga ctttttccaa actgaaccag
     3001 tatctctcat gttttttcct ccttttcttt acttgcaggc aaacaaaagg tgcaaacccc
     3061 tcgtttctgg atgatgattt tattcgtttc gccttatagc tttggttact acgatgagta
     3121 ttccttaatc atcattagac aaacggtgtt tacatgctgc ttacatgctt tgttataata
     3181 acttaaatta taatttgtgt caatctaaat tgtgttaaac tccttataaa ggagtgtttt
     3241 ttattctttt gggtcatttt ctttaattac atgacaataa gtctcgcaac ttttagcgta
     3301 agagcacgag taacatttct ttttattacg gcgcacaaga tagaaaataa tcccacctaa
     3361 aaggatgatc acaacgatta aaatgataat atccgcagca ctcataataa accctccacg
     3421 aaagtcccaa cttgaaagat taaagtggca atgactaacg ctgctattgt ttgcagaagc
     3481 agcgctttaa tcatagtccg acgattacca aattcatgat tcatagcccc aatcgctccg
     3541 aaacacggcg cactaaataa attaaaggcc ataaaagcat aagctgaagc aggagtaaag
     3601 aaattaaaaa gcggactttc aaagatggtg ttgcccccac taagtttagc aataattgaa
     3661 agtgaagaaa caacttgttc tttggcaatt agcccttgaa taatactaac cgcagccgcc
     3721 caactatttg tgcctaagaa cgggtaaaac acaaacgaga ataagcgtcc aatatcactt
     3781 aaaatactct tctcaatcgc aacgccaaat tgcatcttcc agttaaagtt aagtaaaagc
     3841 cataagataa cagaggcgaa taaaataact gagccggcat tcgtgataaa tgaccatgtt
     3901 ttatgtcctg tatcacgtaa tacatagcgc atatttggtg ctttataagt tggtagttct
     3961 tgaataaatg gtgaatactt ttgcttataa aggatgcgtt ttaaaagcaa agctgttagt
     4021 aaaataatga taattgatag gaaatataat gaggcactta ctagtcccat tgaatcaggg
     4081 aagaagtagg ccgcaaagag cgtaataatc ggtaattttg cgccgcacgg aataaaaggc
     4141 gtaagtaaaa cagttaattt tcgttcgcgt tcatcttcaa taacgcggct tgccataatc
     4201 gccggtacac tgcatcccgt accaataata aaagggataa gtgctttacc cgataaacca
     4261 actttacgga aaacacgatc aaaaaagaaa gcaatgcggg acatataacc acttgcttct
     4321 aagatatcaa tcataaagaa taaaatgagt aattgcggca caaaactaag aatcgaagtc
     4381 acgcctgtta agatgccatc aacgactaaa ctaacagacc acgcagaagc cccaacatta
     4441 gtgagaaaag ttgctaaacc ttcgtttaac gcggcaaaac cctgctcaac tagtgatgag
     4501 agaaaaatgc caactggtcc tgctgctaag aaatagacaa gtaacatgat aataataaat
     4561 atcggaattg ctagatattt atttagtaaa atccgatcaa ttttatccgt taaaagtttt
     4621 ggtttttcgc ggcttgaaac agcaatatca cgaacttttt caataaaatt atagcgctca
     4681 ttcgctaata cttcgcttat cccaagtttg taatgatcat ttaatttttt aatgtctttg
     4741 cttacatcaa gatcgatgcg tggcgctaaa tcaatatcat tttctaatag ttttattgct
     4801 aaaaattctt tagcgtcaat cggtaaaagt tcggctgttt tattgatagt ttcttgcagt
     4861 ggattgctat atatagttag gtaattagta gaagtagggg tatctttagt aattaacatt
     4921 attaaatctt ttatccctgt tctttttaaa catgatattg ggactacggg aacacttagt
     4981 gccttgctaa gtttatttac atcaatccga atcccttgtt caaacgctaa gtcagtcatg
     5041 tttagtgcaa tgaccattgg aatattaaaa ttaaataatt gcgtagtaag atataagcta
     5101 cgttcaatcg cggtactatc aataatgtta attattaagt caactttttc ttctaataag
     5161 aagcgccgga caatattttc ttcatgacta tatggagaga gactatagat acctggtaaa
     5221 tcaatcactt cggcatttga ggtgcgtaaa ataccagttt tgcgctcaag cgttactcct
     5281 ggccaattac caaccttctg attattacca gttaacaaat taaatagtga agttttacca
     5341 ctattttgat taccaattag tgcaattttc attcgataac ctcgacttca atcgcactca
     5401 catcagcgcg acgcactaat aagcgatagt tgcgtaactc taggcaaaaa gggtcaccaa
     5461 gtggtgatat tttatcaatt ttaacaatga caccagtcgt aattcccata tctaaaagtc
     5521 gttgttttaa tgctggatta tcatcattaa ttcttaaaac acgggctttt tgcccgatgg
     5581 taagtttatc tagagtaaga gtatcgctca cttaggaata atcccttcta gtggttatac
     5641 aaagtataat cacacaccaa aatataccct aatagccgct tattatcaag tatttaacat
     5701 aataaatata tgttaaccgt taaaggaaaa ggttaaattt acatccttct tgtcttcatt
     5761 ataatgttta t
//
LOCUS       DAOSLP010000054         5530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_13198_length_5530_cov_9.339178, whole genome shotgun sequence.
ACCESSION   DAOSLP010000054 DAOSLP010000000
VERSION     DAOSLP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13198_length_5530_cov_9.339178"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1129
                     /gene="trpS"
                     /locus_tag="PK460_04680"
     CDS             149..1129
                     /gene="trpS"
                     /locus_tag="PK460_04680"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOE54402.1"
                     /translation="MMRVLSGIKPTGKLTLGNYIGALKNFSQYQSDHEVFIFIADLHA
                     LTLPIDPDELRNNTKLLAAFYLAAGLDPNKVTIFKQSDVSAHAELNAILQNYLYMGEL
                     SRMTQFKEKSQSMDHSGIGLGLFAYPVLMASDILLYEANIVPVGEDQIQHIEITRDLV
                     VRFNNRYGKVLVMPEPQVTRVGKRIMSLSDPSVKMSKSESGKGDIYLTDDLKTMRKKI
                     MSAVTDSGSEIYVDEENKPGITNLLTIYAALTDKSLESCAAQFKDYRYGDFKKEVANV
                     VIDTLGPFQAKAMSFLESGEVDRVLSEGAKKATVIANKTLVKVKKAVGLL"
     gene            1126..1938
                     /locus_tag="PK460_04685"
     CDS             1126..1938
                     /locus_tag="PK460_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOE54403.1"
                     /translation="MNKDKYLIAIDLDGTLLTDDKKITPKTRNYLNALHDEGHLIVIA
                     TGRPFRAAYGFQQQLGVNGPLVTYNGSLTSNPHDASFPTRTRKFDRFALKDMVDAIGF
                     DALDNLMIETEHVIYLLHEDEALNNFFWNDRERIIYGNPFLHLNEDPLALIFLFKNTD
                     PEYKKEVSRIVESFPDLHIRFWNLEGYAELWIGESTKKDGLEHIANFYNFPQKRIIAF
                     GDASNDKEMLAWANYGVWMKNGVPSVEAYANMKTTEDNNNDGIVSTLQKIIK"
     gene            complement(1928..3622)
                     /locus_tag="PK460_04690"
     CDS             complement(1928..3622)
                     /locus_tag="PK460_04690"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOE54404.1"
                     /translation="MQNIDALYEKWLKHPEMPKKLVSELKKMSAKAKTDAFYQNAEFG
                     TAGMRGILGAGTNRINIFTIRKAAYGFGLYVIKHVRNAKERGIVIAHDNRHMSEEFTE
                     ESMKTLRSLGIKTFVFDELKPTPLLSYAVRHLNAAGGIMITASHNPKNYNGFKVYDDT
                     GAQLTPEKVDLLIAELKTLPDYLDIKIDDKVNGYILLSDDVELTYYSKVMKIQLRRDL
                     PKKDFKIVFSPQHGTGRKLGMRALNVIGYNVIPVTEQMEPDPNFSGTKSPNPEEKAAY
                     SEAIKYARAYGAHLIITTDPDADRLGIVAKNKKGDFDYLTGNQSAALLLDYLCKTNIE
                     LGKDIKGKYLYTTIVSSSLANEVAESYGLKVKEFLTGFKFIGAQIAEDDSKNEDYFFF
                     GFEESYGLLAAPFVRDKDAIQAIVLYSEMALYYYLQGKTLGEVLDELYKKYGYHFDVT
                     KSVAFPGAAGQSEMQEILKRLRDNPPVTIGGLNILRYDDYLTSKSVTSGETTTLTLPK
                     SNVIRYHLEGGTTISIRPSGTEPKCKIYYGIITENEKEAQDLLTLLEADIKVRFTL"
     gene            complement(3626..4549)
                     /locus_tag="PK460_04695"
     CDS             complement(3626..4549)
                     /locus_tag="PK460_04695"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE54405.1"
                     /translation="MEKLTFIISDIEANQRLDKYLKKIMPNAPQSFIYKLIRKKDIRV
                     NNKKSDAAYILNEDDTVVIFLTEAQVEEFIVPYTFTKIRRPLNIIYEDENILVVNKDT
                     GLLVHGTEFENELTLTNIVLTYLYDRGEFDPLRRGYIPSPVSRIDRDTSGVVIFSKKQ
                     SVHQVLANAFTQEDEVIRLYRLVVYGTLNKDEGAIELPLKRENGQTIPASDGQKAITH
                     YVVKKKGRTKTYVEAQLLTGRQHQIRAHFKAIGHPLVGDTKYGSSTEQGPLSLNAYKV
                     EFTALPAPLDYLNGHEFIADNTAYLLEMLGD"
     gene            4612..5406
                     /locus_tag="PK460_04700"
     CDS             4612..5406
                     /locus_tag="PK460_04700"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOE54406.1"
                     /translation="MFKKEGFAEPAGLFSLSHLGLVLFFGTLAVLFYALTKKWTKAQV
                     MRFFKIYSLVLIALEIFKIIWNIVNYGTSAESLNRFIPLYYCSIFLYAFVIVAFTKGK
                     IQKAAYTWIMYGGLASGLAFLIYPSSSLLFYPFYHFLCLHSILFHMNFILIGFLIFRH
                     ELYTPNVKDFWYFVSFSLVFMSLAVILNKVLGTNLMFFEKPLAILPFETLNEWSPLFF
                     QSLIFILQLFGPFIGTHFVFYLFSRFTPFKIYKRNEENLTTNNDNI"
     gene            5435..5525
                     /locus_tag="PK460_04705"
     tRNA            5435..5525
                     /locus_tag="PK460_04705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5469..5471,aa:Ser,seq:gga)
BASE COUNT         1711 a         1044 c          944 g         1831 t
ORIGIN      
        1 cacataaaat cattaaaaca cttaatcttg ttaattttaa ttattcatct atccacctga
       61 aatgaatttt aattaatttt tgacaatgta tgcgcttagt tgactataaa aataatatag
      121 attataattt ttaagattaa aaggagtaat tatgcgagta ttaagtggaa ttaaaccaac
      181 cggaaaacta acacttggta actacattgg ggcgttaaaa aacttctccc aatatcaaag
      241 tgaccatgaa gttttcatat tcattgccga cttacacgcc ttaacgctgc cgatagaccc
      301 tgatgagtta cgcaataata caaagttatt agcagccttc tatctagcag ccgggcttga
      361 tcccaataaa gtaacaatat ttaaacaaag tgatgttagt gctcacgctg aattaaatgc
      421 gatcctacaa aactatttat atatgggtga acttagtcgt atgactcaat ttaaagaaaa
      481 aagtcaatca atggatcata gcggtattgg cttaggttta tttgcctacc ccgtcttaat
      541 ggctagcgat attttacttt atgaggctaa tatcgtgccg gtcggcgaag accaaattca
      601 acatattgaa attacacgcg acctagttgt gcgctttaat aaccggtacg gaaaagtttt
      661 agtaatgccc gagccacaag taacgcgtgt cggtaaacgc attatgtcac taagtgaccc
      721 gagtgttaaa atgagcaaaa gcgaaagcgg taaaggtgat atatatctaa ctgatgactt
      781 aaaaacaatg cgcaagaaaa ttatgagcgc tgttactgat tccggtagtg aaatttatgt
      841 tgatgaagaa aataaacctg ggattacaaa tttacttaca atttacgctg cgctaactga
      901 taaaagcctt gaatcatgcg ctgcgcaatt taaagattac cgttatggcg actttaaaaa
      961 agaagtcgct aatgttgtta ttgatacact tggtcctttc caagcaaaag caatgtcttt
     1021 tcttgaaagc ggggaagttg atcgcgtcct aagtgaagga gcaaaaaaag caacagttat
     1081 cgctaataaa acattagtga aagtaaagaa agcagtagga cttttatgaa taaagacaaa
     1141 tatttaattg ccattgatct cgatggcaca ctcttaactg atgataaaaa aatcacccct
     1201 aaaactcgca actatctaaa tgcccttcac gatgaaggac atttaattgt catcgccacc
     1261 gggcgaccat ttcgcgcagc atatggtttt caacaacaat taggagtaaa cgggccactt
     1321 gttacatata acggctcttt aacatctaat ccacatgatg cttcattccc aacacgcaca
     1381 cggaaatttg atcgctttgc gcttaaagat atggtagacg ctatcgggtt tgatgcctta
     1441 gataatttaa tgattgaaac tgaacatgtt atttatttat tacatgaaga tgaagccctt
     1501 aataatttct tctggaatga tcgtgaacgc ataatttatg gtaacccctt cttacattta
     1561 aacgaagacc ctttagcgct tatattctta tttaaaaata ctgatcctga atataaaaag
     1621 gaagtatcgc gtattgtcga atcattccct gatttacata ttcgtttctg gaatttagaa
     1681 ggttatgcag aattatggat tggcgaaagc acaaaaaaag acgggcttga acacattgct
     1741 aacttttata atttcccaca aaagcgcatc attgcttttg gcgacgcatc taatgataaa
     1801 gaaatgctag cgtgggcaaa ttatggtgtg tggatgaaaa acggtgtacc aagtgttgaa
     1861 gcttacgcaa atatgaaaac aactgaagat aataataatg atggtatcgt aagcacctta
     1921 caaaaaatta taaagtaaag cgaactttaa tatcagcttc aagaagcgta agtaaatctt
     1981 gcgcttcttt ttcattttca gtaataatac cgtaataaat tttgcacttt ggttcagtac
     2041 cacttgggcg aatcgaaatc gttgtgcccc cttcaaggtg ataacgaatg acattactct
     2101 ttggtaatgt taaagttgtt gtttcgccac ttgtcacact tttactagtt aaataatcat
     2161 cgtagcgtaa aatatttaac ccaccaatcg tgacgggcgg gttatcacgt aatcgtttta
     2221 agatttcttg catttcactt tgaccggcag cacctgggaa agctactgac ttagtaacat
     2281 caaagtgata accatatttt ttgtaaagtt catctaaaac ttcaccaagt gtcttgcctt
     2341 gtaagtaata atataaagcc atttcactat aaaggacaat tgcttgtatc gcatctttat
     2401 cacggacaaa tggagcggcg agtaacccgt aactttcttc aaaaccaaag aagaaataat
     2461 cttcgttttt actgtcatcc tcggcaattt gtgccccaat gaacttaaag ccagttaaaa
     2521 attctttaac ttttaaacca taactttccg ctacttcgtt agcaagacta ctacttacaa
     2581 ttgtcgtgta taagtatttt cctttaatat ctttgcctag ttcaatattc gttttacata
     2641 agtaatctaa aagtagcgct gcactttgat tacctgttaa ataatcaaaa tcgcctttct
     2701 tatttttagc tacaatccct aaacgatcag cgtcaggatc agtcgtaata attaaatgcg
     2761 caccatatgc tctagcgtat ttaattgctt cgctgtaggc tgctttttct tcgggattag
     2821 gactttttgt gccggaaaaa ttcgggtctg gttccatttg ttcggtaaca ggaataacat
     2881 tatagccaat aacattaagc gcccgcattc caagttttct ccccgtgcca tgctgcggtg
     2941 aaaagacaat tttaaagtcc ttcttcggta agtcgcggcg taattgtatt ttcattactt
     3001 ttgagtaata agttaattca acatcatcac ttaaaagtat atatccatta actttgtcat
     3061 cgatttttat atctaaatag tcaggtagcg tttttaattc ggcaattaat aaatcaactt
     3121 tttccggtgt aagttgtgca cctgtatcgt cataaacttt aaagccgtta tagttttttg
     3181 ggttatgcga agcggtaatc attattccgc ctgctgcatt taaatgccgc actgcataac
     3241 ttagtaatgg tgtaggtttt aattcatcaa acacgaaggt tttaattcct agactgcgca
     3301 atgttttcat tgattcttcc gtaaattctt cactcatatg acggttatca tgggcaataa
     3361 caatcccgcg ttctttagcg tttctcacat gtttaatcac atataaacca aagccatatg
     3421 ccgctttacg aatcgtaaaa atattaattc tatttgtacc cgcgcctaaa attccgcgca
     3481 ttcccgctgt gccaaactcg gcattttgat aaaaggcgtc tgttttagct ttcgcgctca
     3541 tcttttttaa ctcgctaaca agttttttag gcatttcagg gtgttttaac catttttcat
     3601 acagtgcatc aatgttttgc ataatttaat ctcctaacat ttctaataaa taagccgtgt
     3661 tatcggcaat aaattcatga ccatttaaat aatcaagtgg ggctggtagt gcagtgaact
     3721 ctaccttata agcgtttaaa gaaagtggtc cttgttcggt actactaccg tatttagtgt
     3781 caccaacaag cggatgacca atcgccttaa aatgcgctct tatttggtgt tggcgccccg
     3841 ttaaaagctg cgcttccaca tatgttttag tgcgtccctt tttcttaact acataatgag
     3901 ttatcgcttt ttgaccatca ctagcaggaa tcgtttgacc attctccctt tttaaaggga
     3961 gttcaatcgc tccttcatcc ttgtttagtg taccataaac gactaaacga tataaacgaa
     4021 tcacttcatc ttcttgcgta aatgcatttg ccagcacttg atgaacgctt tgcttcttac
     4081 taaaaataac aacaccactt gtatcacgat caatacggct gactggcgaa gggatataac
     4141 cgcgccgcag cgggtcaaat tcaccacgat cataaaggta agttaacaca atattagtta
     4201 aggttaattc attttcaaat tcagtaccgt gcactaaaag tcctgtatct ttattaacca
     4261 ctaaaatatt ttcatcttca taaattatgt taagtggtcg tcttattttc gtaaaagtat
     4321 aaggcacaat aaattcttca acttgcgctt ctgttagaaa aatgacgacg gtgtcgtctt
     4381 catttaatat gtaagcagca tcactcttct tattatttac tctaatatct tttttacgaa
     4441 ttaatttata aataaaactc tgcggggcat taggcataat cttttttaaa tacttatcaa
     4501 gacgttgatt agcctcaatg tcactaatga tgaatgttag tttctccata ctttaatttt
     4561 accaaatatc agtgcgcata tggtaaaatt agacaatgaa aacgagggca aatgtttaaa
     4621 aaagaaggct ttgctgaacc tgcgggactc tttagtctta gtcacctcgg attggtgctc
     4681 ttttttggga cgcttgcagt gcttttttat gcattaacga aaaaatggac aaaagcgcaa
     4741 gtgatgcgtt tctttaagat atatagtctt gtattaatag cgttagaaat atttaaaata
     4801 atttggaata tcgtaaatta tggcactagt gctgagagtt taaatcggtt tatcccgctt
     4861 tattattgct ccatttttct ctatgctttt gtaattgttg catttaccaa aggtaaaatt
     4921 caaaaggcgg cttatacttg gataatgtat ggcggacttg cttcaggttt agccttttta
     4981 atatatcctt ctagttcgct tttgttttat cctttctacc acttcttatg tttgcattct
     5041 atcttattcc atatgaattt tatcttaatt ggttttttaa tcttccgtca tgaactctac
     5101 actccaaatg ttaaagactt ttggtacttt gtgagctttt cactcgtatt tatgtcacta
     5161 gcggtaatct taaataaagt gttaggcact aatttaatgt tctttgaaaa acctttagcg
     5221 atccttccgt ttgaaacatt aaacgaatgg tcaccactct ttttccaatc cttaatcttt
     5281 attctgcaat tatttggacc ttttattggc acacactttg tcttttatct ctttagtcgt
     5341 ttcactccat ttaaaatata taaaaggaat gaagaaaact taactacaaa taatgacaac
     5401 atataattaa atttgctata ataaccgcac tgctggagaa atacccaaga ggctgaaggg
     5461 gctggcttgg aaagctagta ggtctgtcac aggacgcgag ggttcaaatc cctctttctc
     5521 cgccagataa
//
LOCUS       DAOSLP010000055         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_15292_length_4922_cov_6.939799, whole genome shotgun sequence.
ACCESSION   DAOSLP010000055 DAOSLP010000000
VERSION     DAOSLP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15292_length_4922_cov_6.939799"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..321
                     /locus_tag="PK460_04710"
     CDS             157..321
                     /locus_tag="PK460_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54407.1"
                     /translation="MTNTNIKLITEKTKHELFILKIDVILVRHGLKRTQIVYDRAGQV
                     AVSFVPPKRK"
     gene            384..1679
                     /locus_tag="PK460_04715"
     CDS             384..1679
                     /locus_tag="PK460_04715"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HOE54408.1"
                     /translation="MNTFKTKWKEGITKIDSFFNMLFDYFMKFFRKYYLWIAFAFILA
                     ISFFVRYAFFDYLSGDMHNFLLQWFNHLKANGGLKAMGDYPWTNTLGVRAGDYPVSYI
                     TLLALLSYLPFEGIVSIKLLSVFFDYVLAIGAVLIIREFNKNSYFSLLAFAILVFFPT
                     SILNSAVWGQCDQLYVALIVWTVLFLIKKKHFLAMIFLGLALATKLQTTFFLPVLIFM
                     WLAKKFKLRYLLVMFLVIFLTFIPSYIAGAPFSMPFNMYRSQIGLYTNANYGAGSLYA
                     FFEFPKLYAGIKRVGVYVAFIAIGVTLLVLFHYKVPATPKNIIYVTALFSLVSPFFLP
                     HMHERYFYMADAFLIIYVLVFKRKYIYAALMSFSSVLTYTHFLTGQYIFKFLDKDCVR
                     LSALINLGLIIALIIDAKSVLEKEPAEELLELETEQSEV"
     gene            complement(1676..4426)
                     /locus_tag="PK460_04720"
     CDS             complement(1676..4426)
                     /locus_tag="PK460_04720"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOE54409.1"
                     /translation="MKSLINKIKCIKKEDISRITFTALPFVLAFFIPFILAIILFSKH
                     GYYPFSADGHSLAMVDLRGQYIAFFRYYKTILDGDNTILYTLGKVTGGDFLSIFTYYL
                     ASPFNLVLKFVANVELPKALMWLVIIKIALSGLTAYISLAAINKNGFVNLIFSIAYAL
                     MAYNFVYYSNIMWLDGVLALPLVALGIHLLTQEKSALLYIFALAYVIATSWYIGIMVA
                     MFSVFFFLYNFISLNSRMYFRKKLLLTFTLSSLLAGLISFAFWGSALVNILGTKGSMS
                     FTNLSKRIKEFYALVDIERGFLYGNHIGMKDIMEPTSVIYAGALPLFLMIMFFANRAF
                     SRKQRIAALTLVLIYVVALFNKGVDHLFHGGPAPNWFPCRYGFIFSFIVIYFASLNFR
                     EYKATKWYAFIAPVIVYLFLIISLTKNEFAYDKKSILYFSITFCVLVIFFVLNEFIFK
                     QKGEEDNSKRVQKYVTASVSFLLIIVSAINVFGNSNRILSVFSDTVYHESMTAYRNDE
                     RIGRAIDFLKKSDNSLYRLEKSFIRSGTYNMANNDSMYFNYNGISHYSSSEKKKVMDY
                     MLKIGFHYNGFNLNYANGSTLAINSYLGVKYLLDNGVNRNFDFVRELPLVDDGLNDDI
                     NTYLNTYALPFLMPIEDTNISYVGDGVYVDGGSSVYWFDMFEYQNHLFKTITRTVKDE
                     LGKPKDIFKKATYTKTLSNVTPLPRDYYYKVVSSGTIDYDITLDKNTNYYYYIHGPKS
                     NDLALYDRNIYTTYFSYHGYQINGIRRNTASTRLSVAITKPQESIKLQEAIYYEDLSV
                     LNEYINAIKAIAEVDIKQEKTSKYSASVVTKKDNQYMLLTLPYNQNLRVFIDGKKVET
                     TTRFNIFTGFTIPKAGAHKITIKYVELTYQLGIPVGILSAAGTIVISVLNKKKKEEQE
                     KA"
     gene            complement(4401..4829)
                     /locus_tag="PK460_04725"
     CDS             complement(4401..4829)
                     /locus_tag="PK460_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54410.1"
                     /translation="MKFKKKVIIKLDTINILMVLSFFLPLATLRKRLPERGLVINYVS
                     GFNMFASPLWPFILPYFIPLLIFIATSFFYFKSKKVRVINFIMLLIMIILGAIAISIF
                     YGYGTLYNIAFLICVVLMITLLVNFKKEEVIQSEITDKQN"
BASE COUNT         1684 a          827 c          766 g         1645 t
ORIGIN      
        1 caaaaattag tcctacttcg tagggctttt tttatatttc atcatagtca tttttaaagt
       61 tatgagatac aataaaacat atacagtggt tagtaacatt aacactaata ttaatgcccc
      121 tcattaataa ccgcgacacg gaaaggggtt ttagctatga ctaacactaa cattaaactt
      181 atcaccgaaa aaacaaaaca cgaattattt attttaaaaa ttgatgtcat acttgtaaga
      241 cacggtttaa aaagaacgca aatagtttac gaccgagcag gtcaagtagc ggtgagcttc
      301 gtgccgccta aaagaaaata gtattccacg caataagtaa tattttaata tatggtaaac
      361 tttacttaag taagaggatg caaatgaata cttttaaaac taaatggaaa gaaggtatta
      421 cgaaaattga ctcatttttt aatatgcttt ttgattattt tatgaagttc ttccgcaaat
      481 attacttatg gattgctttt gcctttattt tagctatttc ctttttcgtc cgttatgcat
      541 tttttgatta tttaagtggc gatatgcata actttctctt acagtggttt aatcacttaa
      601 aggctaatgg ggggttaaag gcaatgggtg attacccgtg gactaacaca cttggtgtta
      661 gagcagggga ctatcctgta tcttacataa cactgctcgc ccttctttcg tatttaccat
      721 ttgaagggat tgtttcaatt aaattactta gtgtcttttt tgattatgta ctcgctattg
      781 gagcggtact tataattcgt gaatttaata aaaactctta tttctcgcta ttagcgtttg
      841 ccattttagt atttttcccc acgagcattc ttaattccgc tgtgtggggt caatgtgatc
      901 aactctatgt tgcgcttatc gtctggacag tattattcct tattaaaaag aaacactttt
      961 tagcaatgat ttttttagga ctagcgttag cgacaaaact acaaacaaca ttctttttgc
     1021 cggtgttaat ctttatgtgg ctcgctaaaa agtttaaact ccgttatctt ttagtaatgt
     1081 tcctcgttat ttttcttacc tttatcccta gctatattgc cggcgctcct tttagtatgc
     1141 cttttaatat gtatcgttca caaattgggt tatatacgaa cgcaaattat ggagcaggat
     1201 cattatatgc tttctttgaa tttccaaaac tttacgccgg aataaagcgt gttggtgttt
     1261 atgttgcttt tatcgcaatt ggcgttacac ttttagtgct cttccactat aaagtaccag
     1321 caacgcctaa aaatataata tatgtcactg cactattttc attagtgtca ccctttttct
     1381 taccacatat gcatgaacgc tatttctata tggcggacgc ttttttaatt atttatgttt
     1441 tagtatttaa acgtaagtac atttatgcgg cactgatgtc tttttcaagc gtcttaactt
     1501 atacacactt tttaactggt caatacatct tcaagttttt agataaagac tgtgtaagac
     1561 tttctgcgct aataaattta ggattaatta ttgctttaat aattgacgct aaaagtgtat
     1621 tagaaaaaga accggcagaa gaattacttg aacttgaaac agaacaaagt gaagtttagg
     1681 ctttttcttg ttcttctttt ttctttttat ttaaaacact gattacgata gtgcccgctg
     1741 cacttaatat accaacagga ataccaagtt gataagttaa ttcaacatat tttatagtga
     1801 ttttatgggc accggcttta gggatagtaa agcctgtaaa aatattaaaa cgcgttgttg
     1861 tctcaacttt tttaccgtca ataaacacgc gtaagttttg attataaggc aaagttaata
     1921 gcatatattg attatctttt ttcgtcacta cactagcgct gtacttagat gttttttctt
     1981 gcttaatatc aacttcagca atagccttaa tcgcattaat gtattcattt aaaacactta
     2041 agtcttcgta atatatcgct tcctgtaatt taatactttc ttgtggttta gtaattgcca
     2101 cacttaatcg tgtactagcg gtgttacgcc taataccgtt aatttgataa ccgtgataag
     2161 aaaaatatgt cgtgtatata ttgcgatcat ataacgctaa gtcattactc tttggtccgt
     2221 gaatatagta ataatagttt gtgtttttat caagtgtaat atcataatca atcgtgccgg
     2281 aactaactac tttatagtaa taatctcttg ggagcggagt aacattactt agcgtctttg
     2341 tataagttgc ttttttaaag atgtctttag gtttacctag ttcatctttc acagtgcgtg
     2401 taattgtttt aaataaatga ttttgatatt caaacatgtc aaaccaataa acactgctac
     2461 caccatcaac atatacacca tctccaacat aactaatatt cgtatcttca atcggcatta
     2521 aaaatggtaa ggcgtaagtg ttaagatatg tatttatatc atcattaagt ccgtcatcca
     2581 caagcggtaa ttcacgcaca aagtcaaagt tacgattcac tccattatca agtagatact
     2641 ttacacctaa atagctatta atcgctaaag ttgaaccgtt cgcataattt aaattaaagc
     2701 cgttataatg aaatccaatc tttagcatat aatccattac tttctttttt tcgcttgaag
     2761 aatagtgtga aataccgttg taattaaaat acattgagtc attattagcc atattatatg
     2821 taccgctgcg gatgaatgat ttctctaaac gatagagtga attatcgctt tttttaagga
     2881 aatcaattgc tctgccaatg cgttcatcat tacgatatgc cgtcattgat tcgtggtata
     2941 ctgtatcact aaacactgac aaaatgcggt tagaattgcc aaatacatta atcgcgctca
     3001 cgattataag caggaaagaa acactcgccg taacatattt ttgtactctt ttagaattgt
     3061 cttcttcccc tttttgctta aagataaatt cattgagcac gaagaaaata actaagacgc
     3121 aaaaggttat actaaaatat aaaatacttt ttttatcata cgcaaattca tttttggtaa
     3181 gcgaaataat taagaataaa taaacgatta ccggagcaat aaaagcatac cattttgtcg
     3241 ctttatactc tctaaagtta agggaagcaa agtaaataac aataaaacta aaaataaacc
     3301 cgtagcggca cggaaaccag ttcggggctg gaccaccgtg gaagagatga tcaacacctt
     3361 tattaaataa cgctactaca taaattaaca ctagagttaa agctgcaatt ctttgtttac
     3421 gactaaatgc acgattagca aagaacataa tcattaaaaa tagtggtaat gcaccagcgt
     3481 aaattacact tgtgggttcc ataatatctt tcattccaat atgattcccg tataggaaac
     3541 cgcgttcaat atcaacaagc gcataaaatt ctttaattct ttttgataag ttagtaaaac
     3601 tcattgagcc ttttgtccct aaaatattta caagcgctga accccaaaag gcaaacgaaa
     3661 ttaaacctgc tagtagtgaa cttaaagtaa aggttaaaag taattttttc cgaaaataca
     3721 ttcgtgaatt tagacttata aaattgtaaa ggaaaaagaa aacagaaaac attgccacca
     3781 taatcccgat ataccacgaa gtagcaatga cataggctaa cgcaaaaatg tataaaagcg
     3841 cactcttctc ttgtgtaagt agatgaatac ctaaagcaac aagcggtaaa gctaatactc
     3901 catcaagcca cataatgttt gaataataga caaagttata cgccattagt gcatacgcaa
     3961 tactaaagat taagttaaca aaaccgtttt tattaatagc cgctaagctt atataagcag
     4021 ttaaaccact tagtgctatt ttaataatga caagccacat taaggctttt ggtaattcaa
     4081 catttgcaac aaactttagc actaaattaa agggggatgc taagtagtaa gtgaaaattg
     4141 aaagaaaatc accaccagtt actttcccta atgtatataa aatagtattg tcgccatcta
     4201 atatcgtttt ataataacgg aaaaaggcaa tatactgtcc gcgcaaatca accatcgcta
     4261 aagaatgccc gtcggcacta aaagggtaat atccatgttt tgaaaataaa ataatagcaa
     4321 ggataaacgg aataaaaaaa gctaagacaa aaggtaaagc agtaaatgtt atacgactga
     4381 tatcttcttt tttaatacac ttaattttgt ttatcagtga tttcactttg gattacttct
     4441 tctttcttaa agtttacaag cagcgtaatc attaacacaa cacaaattaa aaaggcaata
     4501 ttatagagtg tcccatatcc gtagaatata ctaatcgcaa tagcgcctag tataatcata
     4561 atgagcaaca ttataaaatt aatgacccgt acttttttac ttttaaagta gaaaaatgat
     4621 gtagcaataa aaataagaag cgggatgaaa taaggtaaga taaacggcca taatggtgaa
     4681 gcgaacatat taaagccgct gacataatta atgacgagcc cgcgttcagg taaacgtttt
     4741 cgtaatgttg caagcggtaa aaagaaactt aaaaccatta atatattaat agtatcgagt
     4801 ttaataatta ctttcttctt aaatttcatg tttcacctct ttgtttaatt atagtatgaa
     4861 aataataaat tgttacatga aaaagataaa gtgacaaaat agaaaataaa gttttaaaaa
     4921 ta
//
LOCUS       DAOSLP010000056         4886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_15439_length_4886_cov_7.420410, whole genome shotgun sequence.
ACCESSION   DAOSLP010000056 DAOSLP010000000
VERSION     DAOSLP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15439_length_4886_cov_7.420410"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..2370
                     /locus_tag="PK460_04730"
     CDS             64..2370
                     /locus_tag="PK460_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOE54411.1"
                     /translation="MDIFTLLEFNEIVSKVKDLTKTELGGEYASLISPFESEEEATYA
                     LNLVSEAESILTRFGALPLAPSHKLAPLLEEARRGNILTPHDFYLISADAMNIVKINY
                     YLKDKLDNAPLLKAMLKREKDLAVLANEINKVITPNLLVKDNASHELARIRKNIALSE
                     DRLNKAAQGLISKYGNFLAENLITKRDGHFVIPVKTAYKNKVDGIIYDISNSGQTIFV
                     EPSEIAALNSTLISYQNDEIIEVNRILRALTLETLKYEDEIINNNILLAELDLIFAKA
                     TYGQSINGFVPVFQQTKGLQFKKARHPLIDKNLVVANTFNLKDDKFIIVISGPNAGGK
                     TVALKTMGLLVLMAKSGLMLPVSEEPKLFFYKNVYASIGDEQSLSQNLSTFSSHVKRI
                     GEIMDVATSDDLVLLDELATGTSPEEGEALALAIATYLLNNSISSVISSHYSRLKEFA
                     YSRSGIINASMAFDEKKLTPLYEYHEEIPGRSYGLVVAKRFGIKDEVIKDAESFLSDG
                     VYDFESTIGKLRNEIRTLELERTELEKERAALLKKEAELDKKLKRQSEAEEDFKLTKN
                     ERLEKEINEAIATLDTLVKSALASGAKAHEIIAVRDEIKKLGKESKKEVKAPAKDFEL
                     GDYVEVKKLHVKGEITRIRGEEYTVTLASGKILKVSASDLEITERTKRKSTPTITVKG
                     LEEDLTSVKPEINVIGMRVEEALNVIGPYLDRALLKRYPSVRIIHGFGTGALRRGIHD
                     YLAKLSFVKSYTLAGEYEGQGGATIVTF"
     gene            2371..4146
                     /gene="uvrC"
                     /locus_tag="PK460_04735"
     CDS             2371..4146
                     /gene="uvrC"
                     /locus_tag="PK460_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOE54412.1"
                     /translation="MKNKKLQFLIDHLPSDPGVYLMLDSHGRIIYIGKAKDLKSRVSQ
                     YFTRPQPPKVAAMVKRTAKFETIVTNSEKEAFILELKLIQTHYPRYNIMLKDDSHYPY
                     IALTKKGDPVVSIKRKTSDDKYIYFGPFPASQKAYQIVDLINKIFMTRKCKGKSKDPC
                     FYFHLGQCPGYCFKDISDLEKDKVRSDILTFLNGKDKDTLKRYETKMKIASAELDFEK
                     ARDYKEIVDAILHIYSSQNIELKNKVDLDVIAFTQINDYIGLNIVSYRAGVLLNEQFD
                     AFPSFSDLHEQIINLIGQYYTKNPLPTKLMVGNSFIAEALTLYYDKEVIVPSRGQNLE
                     ILNRAYQNATSALQKHLLYHQALGTENESLTALSETLGIKYPSHIDFVDIAHLFGSNA
                     QGVVITYINGRPFKKLYRKYNIENENSFDDYASIKEVLTRHYKRKLIEGKHLPDLLIV
                     DGGKGQLGVALSVKDELNLDFNIISLVKDDKHTTKALINEEGDEISVNKNLLTFLAAI
                     QNEGHRYAITSHRAKRGKGTYTSALDGIKGLGKKRKMLLLNTYKSIDVLKSVPLTELS
                     QLIPREVAEALLTKLNRVDDTEGGV"
     gene            4148..>4886
                     /locus_tag="PK460_04740"
     CDS             4148..>4886
                     /locus_tag="PK460_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54413.1"
                     /translation="MTKVKTKKGPKIILAVASLLVVTILSLSIVRIVKNNPPKHKDYA
                     IYLYGEEHGVAKINKKELALWDDFYHKQGMRHLFNESAYFDAAILNLWMQEEDDTRFE
                     FLYDGWEGTLSHNKETRDFYFAFKESYPETIFHGTDVGHQHERAGQYYLDYLIDNGLE
                     NTEAYTLTLESIAQGEEFTKTGDLKYRESKMVSNFIREFDALDNEVIMGIYGSAHISK
                     DFIAFFLGIEPMTIQLKKYYEPQNILIY"
BASE COUNT         1723 a          804 c          945 g         1414 t
ORIGIN      
        1 ttttaacata aacgacaatc ttgttcgtta ttagaaaata atcattaatg ataaaataca
       61 aatatggata tatttacact tttagaattt aatgaaatcg ttagtaaagt taaagattta
      121 acaaagacag aattaggtgg tgagtacgct agtcttatta gtccttttga gagtgaagaa
      181 gaagcaactt acgcacttaa tttagtaagt gaagcagagt caattttaac acgctttggt
      241 gcgttgccac tcgctccaag tcataaactt gccccgcttc ttgaagaagc acggcgcggt
      301 aatattttaa caccacacga cttttattta ataagtgcgg acgctatgaa tattgttaag
      361 attaattatt acttaaaaga taagctagat aatgctccct tattaaaagc aatgcttaaa
      421 cgcgaaaaag atttagcggt gctcgctaat gaaattaata aagtaattac gcctaatctt
      481 ttagtaaaag ataatgcgag ccacgaatta gcgcgcatta gaaaaaacat tgccttgagt
      541 gaagatcgcc ttaataaagc agcgcaaggt ttaattagta agtacggaaa cttcttagcg
      601 gaaaatctta ttactaagcg cgatggtcac tttgtgattc ccgttaaaac cgcctataaa
      661 aataaggttg acggcattat ttatgatatt agtaattccg gccaaacaat atttgttgaa
      721 cctagtgaaa tcgccgcact aaatagtact ttaattagtt atcaaaatga tgaaataatt
      781 gaagttaatc gcattttaag agcattaact ttagaaactt taaaatatga agacgaaatt
      841 ataaataata acatcttact tgcggaactt gatttaatct tcgctaaagc gacttatggt
      901 caaagtatta atggctttgt ccccgttttt caacaaacaa aagggttaca atttaaaaaa
      961 gcccggcacc cgcttattga taagaatctt gtcgttgcta atacttttaa tttaaaagat
     1021 gataaattca tcattgttat aagtgggcct aacgctggtg gtaaaacagt agcgcttaaa
     1081 acaatgggtc ttttagtgct gatggcaaaa agcgggctca tgttacccgt aagcgaagaa
     1141 cctaaattat ttttttataa gaatgtgtac gctagtattg gcgacgaaca aagtttaagt
     1201 caaaatcttt cgacattcag tagtcatgtg aagcgtattg gggaaataat ggatgttgcg
     1261 actagtgacg accttgtatt acttgatgaa ctcgcgactg gcactagtcc tgaagaaggg
     1321 gaagcacttg ccttagcaat cgctacttat ttattaaata acagtattag cagtgtaatt
     1381 agcagtcact atagtagact taaagaattt gcctactccc gcagtggcat cattaatgct
     1441 tcaatggcgt ttgatgaaaa gaaattaaca ccgctttatg aatatcatga agaaatacca
     1501 ggacgctcct acggtttagt tgtcgctaag cgctttggga ttaaagacga agttattaaa
     1561 gacgcggagt cctttttaag tgacggcgtt tatgactttg aaagtacaat tggtaaatta
     1621 cgcaatgaaa ttagaacact tgaacttgaa cgcactgaac tagaaaaaga gcgcgcagca
     1681 ctccttaaaa aagaagccga attagataaa aagcttaagc gacaaagtga agcagaagaa
     1741 gactttaagt taacgaaaaa tgaacgctta gaaaaagaaa ttaatgaagc aattgcaaca
     1801 cttgatactt tagtaaaaag cgcacttgct agtggcgcaa aagctcacga aatcattgcc
     1861 gtgcgtgatg agataaaaaa acttggtaaa gagagtaaaa aggaagtaaa agcacccgct
     1921 aaagacttcg aactaggcga ctatgttgaa gttaaaaaac tccatgttaa aggcgaaata
     1981 acgcgtattc gtggcgaaga atatactgta acgctcgcaa gcgggaaaat acttaaagtt
     2041 agtgcaagtg accttgaaat aacggagcgc actaagcgta aaagcacacc aacaattacg
     2101 gttaaaggtt tagaagaaga cttaactagt gtcaaacccg aaattaatgt gattgggatg
     2161 cgtgttgaag aagcgcttaa tgttattgga ccctaccttg accgcgctct acttaaacgc
     2221 tatccaagtg tccggattat tcacggcttt ggtaccgggg cactgcggcg cggtattcat
     2281 gactacttag cgaaactttc atttgtaaaa agttacactc tcgccggaga atacgaaggc
     2341 caaggagggg cgacgatcgt caccttctaa atgaaaaata agaagttaca atttttaatt
     2401 gaccatttac cgagtgaccc tggtgtgtat ttaatgctag atagtcacgg tcgcattatt
     2461 tatattggca aggcaaaaga cttaaaaagt agagtaagtc aatattttac gcgtccccaa
     2521 ccaccaaaag tcgcggcaat ggtaaagcgc accgctaaat ttgaaacaat tgtgacaaac
     2581 agtgaaaaag aagcatttat tcttgagctt aaattaattc aaacgcatta tccgcgctat
     2641 aacattatgc ttaaagatga ttcgcattac ccatatattg cacttactaa aaagggtgat
     2701 ccggtcgtta gtataaaacg caaaacaagt gacgataaat atatttattt tgggccgttt
     2761 cccgcgagtc aaaaggcgta tcaaatcgtt gatttaatta ataaaatctt tatgacacgg
     2821 aagtgtaaag gtaaaagtaa agatccgtgc ttttactttc atttagggca gtgtccgggg
     2881 tattgcttta aagatataag tgacttagaa aaagataaag tacgaagtga tattctcact
     2941 tttttaaatg gtaaagataa agatacatta aaacgctatg aaacaaagat gaaaatagca
     3001 agtgcggaac ttgactttga aaaagcgcgt gattataaag aaatagttga cgcgatactc
     3061 catatttata gcagtcaaaa tattgaactt aaaaataaag ttgatttaga tgtgattgcc
     3121 tttacccaaa ttaatgatta tattgggcta aatattgtct cttatcgtgc tggagtatta
     3181 cttaatgaac agtttgatgc ctttccctct tttagcgacc ttcatgaaca aattattaat
     3241 ttaattgggc aatactacac aaagaatcca ttgcccacta aattaatggt cggtaattca
     3301 tttatcgctg aagcgttaac gctttattac gataaagaag ttattgtccc tagtcgcgga
     3361 caaaatctag aaatccttaa tcgggcttat caaaatgcca caagtgcatt gcagaagcat
     3421 ttactatatc atcaagcttt aggcactgaa aatgaaagtt taacggcgct aagtgaaacg
     3481 cttggtatta agtatcctag tcatattgat tttgttgata ttgctcatct ttttggtagt
     3541 aatgcccaag gagtcgttat tacttatatt aacggtcgac catttaagaa gttatatcgt
     3601 aaatataata ttgaaaatga aaattctttt gatgattacg ctagtattaa agaagtactc
     3661 acacgccact ataaacgaaa actcattgaa ggaaaacatt tacctgattt actaatcgtt
     3721 gatggcggta aaggtcaact tggtgttgcg cttagtgtga aagacgaatt aaatcttgat
     3781 ttcaacatta ttagccttgt gaaagatgat aaacatacaa caaaagcact tattaatgaa
     3841 gaaggtgacg aaataagtgt taataaaaat ttactcactt ttctcgccgc gattcaaaat
     3901 gaagggcacc gttacgcgat tactagtcac agagctaaaa gagggaaagg gacatacaca
     3961 agcgctttag acgggattaa aggtttaggc aagaaacgga agatgctctt actaaatact
     4021 tataaaagta ttgatgtctt aaaaagtgtc ccactaacag agctaagtca acttatcccg
     4081 cgcgaagtag cggaagcctt acttactaaa ttaaataggg ttgatgacac ggaaggagga
     4141 gtttaatatg actaaagtaa agactaaaaa aggaccaaaa ataattcttg ccgttgcctc
     4201 actcttagtt gttactattc tttctttaag cattgtacgc attgttaaaa ataatccgcc
     4261 caagcataaa gattatgcca tttatctcta tggcgaagaa catggggtcg ctaaaatcaa
     4321 taaaaaagaa ctagcgctct gggatgactt ttatcataag caaggtatgc gtcatctttt
     4381 caacgaatca gcctattttg atgcagcaat tcttaattta tggatgcaag aagaagatga
     4441 cacacgcttt gagtttcttt atgatggttg ggaaggaaca ttatcgcata ataaagagac
     4501 ccgtgacttt tattttgcct ttaaagaaag ttatccagaa acaatattcc acggcactga
     4561 tgttggccat caacatgaaa gagcaggaca gtattactta gattatctta ttgataacgg
     4621 tttagaaaac accgaagcat acactttaac acttgaatca atcgctcaag gcgaagaatt
     4681 tactaaaact ggcgacttaa aatatcgtga aagtaagatg gtaagtaact ttattcgtga
     4741 atttgatgct ttagataatg aagtgataat ggggatatat ggtagtgcac atattagtaa
     4801 agactttatt gctttctttt taggcattga accaatgaca attcaactta aaaagtatta
     4861 cgaacctcaa aatatattaa tatacg
//
LOCUS       DAOSLP010000057         4858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_15539_length_4858_cov_8.179263, whole genome shotgun sequence.
ACCESSION   DAOSLP010000057 DAOSLP010000000
VERSION     DAOSLP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15539_length_4858_cov_8.179263"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK460_04745"
     CDS             complement(<1..532)
                     /locus_tag="PK460_04745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOE54414.1"
                     /translation="MRNKQLRILLAVTLTLNIILAVIKLGAGYLYNSPSLVGDGFNSF
                     FDIFISVMLLIVMKTASRAADENHHYGHQKYEGLIFFGLGLFILFTTVVLGINTVKDF
                     IAYFQGAEYVLPGTPALIVAIVSVFIKVFMFIINYRAYLKLKRPALKGDAINHLSDIF
                     ATAVSIISIVLAKFNFY"
     gene            646..3588
                     /locus_tag="PK460_04750"
     CDS             646..3588
                     /locus_tag="PK460_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE54415.1"
                     /translation="MLELVNIKRYYTQKKGPTVKALDDVSLVFPETGMVFVLGKSGSG
                     KSTLLNVIGGLDQATSGDLIIKGKSTKKFKQNEFDSYRNTMVGFIFQEYNVLNDFTVG
                     QNIGIALELQGEKASNEKINAMLELLDMQGYGHRKPNTLSGGQKQRVAIARALVKNPQ
                     IIMADEPTGALDSVTGKQLFETLKKLSQDKLVIVVTHDHEFAENYGDRIIEFSDGKVI
                     RDVSKVKNEKQETNTVFSEEGITVSAGYELTAEDLVKINEYLRQNRDKTFIKVLNNDY
                     EFKPTKQPASFPKTEKLDLIKSKMPFKSALKIGASALKHKRFRLALSIILATSAFAMF
                     GLTDAIANFKRTETTINTMLDLNIGYSSISKIERIKYSDYYYSQQVGLDDDDLAELEK
                     IDNRLDFFPVLNEALEYSSNFYSDSFDYYDPYRYGINTIAHLKDADLTKANLSLLAGT
                     LPTNKNEVAVPEYVFDSFKKYGYRSLTNSTKIAIDDPEDLLNKTLNNKFVISGVYKTG
                     LEVDRFIIENVDDDGSIYNTINTEVFRQYLLNGFHTTLLVHEEYYNKLVEQNKVYSLP
                     FNSNSYFQMGDVDRYQFTTRNFRRIESLKTNNDVILFNGITHDNLGRDDIILSTSAAF
                     FQGMEGGVHDSVQTALLVFVDNIKSTDENDLAIEQMLKTNYQHYGFNSPLDVTAPSLW
                     SEQEKTSFRNFYAQALLTFLYDENPFQPELIGREAIFAVTSAQIKTDFAPFNIEYFLV
                     AYESDGEKLKAAQDLRVVGFTFDYQYYIEAWESIIIYINEENAEVMNLTPEGAYFRAV
                     TYHGNSRAIIQKLVKFNELGTKKDVGSFYRMNNEVSYLVENVGDFLEIISRIFVVVGI
                     IFAVFASLLFLNFITLSVAFKKQEIGILRAIGARGIDVATIFMNEAGIIALINLILSF
                     MLAIIAGTTINNYIGKNINIYLSIVNVGLRQLLLLILIAFGVAFISSFIPVFRLSRKK
                     PIDAIRMR"
     gene            complement(3654..4691)
                     /locus_tag="PK460_04755"
     CDS             complement(3654..4691)
                     /locus_tag="PK460_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOE54416.1"
                     /translation="MGIRFSVKSKIREWLSFRPRLKVATEWMYLLMLVLFSSFTYALA
                     FRLFISPSLETLHSVSGNQIVFVGGGVSGLSQNLVKLIHDIIGWQKVNPRVLQSILYF
                     TLNIPVFILGWLKIGRRFTIFSIINVLLASLFISIIPVSWEKAVIYDSQLTRTLYAGI
                     LAGFSAAVAFSGNVSSGGMDIIAYYFANKKSEGVGKYNVLFNVVVVGFYFVLNLIHPS
                     TNIDIANTQFTNTNVAITSFISSVTYLVMNSFVIDLINVRNKKSQVQIITNDEELAKI
                     LISNFPHGATTIKGIGVYSQSEKSVIYMTVSSNEVNDVVRLTQQVDPKAFINVYDLRQ
                     VFGKFYISPIK"
BASE COUNT         1698 a          851 c          802 g         1507 t
ORIGIN      
        1 tataaaaatt aaatttagca aggacaatcg aaataatact gaccgctgtc gcaaaaatat
       61 cagaaagatg attaatcgcg tcccccttta aagccggccg ctttagtttt aaataagcgc
      121 gataattaat aataaacatg aatactttta taaagacact gacaatcgct acaattaagg
      181 ctggtgtgcc aggtaaaaca tattccgcac cttgaaagta agcaataaag tctttaacgg
      241 tattaattcc taacactact gtcgtaaata aaataaatag acctaaacca aagaagataa
      301 gtccttcata tttttgatga ccataatggt gattttcatc agcagcgcga ctcgcagtct
      361 tcatcacaat aagtagcatc acactaataa aaatatcaaa aaaggaatta aagccatcac
      421 caacaagcga aggtgaatta tataaatatc ccgcaccgag cttaatcacc gcaaggataa
      481 tatttaaggt caatgtgacc gctaaaagga tgcgtagttg tttgtttctc atattttgta
      541 ttatagtact tttcaaatgc taattaaatt gattaatcat aaaactacta tatttctata
      601 taatgctata atttattata gaaattagtg ataaggaatt ttattatgct agaacttgtt
      661 aatataaaaa gatattatac gcagaagaaa ggcccaactg ttaaggcgct tgatgatgtt
      721 tcactcgtat ttccagaaac agggatggtg tttgtattag gtaaatcagg ttcaggaaaa
      781 tcaactttac ttaatgtcat cggcggactt gaccaagcga caagtgggga cttaatcatt
      841 aaaggtaaat ctaccaaaaa atttaaacaa aatgaatttg atagctaccg caatacaatg
      901 gtcggtttca tcttccaaga atacaacgtt ttaaatgact tcactgtcgg tcaaaatata
      961 ggcattgcgc ttgaactaca aggtgaaaaa gcatcaaacg aaaaaattaa tgcaatgctt
     1021 gaattacttg atatgcaggg ttacgggcac cgcaaaccaa atacactttc cggcggacaa
     1081 aagcaacgag tagcgatcgc ccgcgctctt gttaaaaacc cgcaaattat tatggccgat
     1141 gagccaaccg gtgcacttga tagcgtcact ggtaaacaac tttttgaaac actaaaaaaa
     1201 ttatcacaag ataaacttgt catcgttgtg actcatgacc acgaatttgc cgaaaactat
     1261 ggtgaccgca ttattgaatt tagtgacggg aaagtaattc gtgatgtttc taaagtaaaa
     1321 aacgaaaagc aagaaacaaa tacggtcttt agcgaagaag gaattactgt tagtgcgggt
     1381 tatgaattaa cagcggaaga tttagtcaaa atcaatgaat atttacggca aaaccgcgat
     1441 aaaacattca ttaaggtatt aaataatgat tatgaattta aaccgacaaa acaaccagca
     1501 tcttttccaa aaacggaaaa attggatttg attaaatcaa agatgccctt taaatcagcg
     1561 cttaaaattg gcgctagtgc acttaaacat aaaagattta gactcgcgct tagtattatt
     1621 ttagccacta gtgcatttgc aatgtttggg ttaactgacg ccattgctaa ttttaaacgt
     1681 acggaaacaa cgattaacac aatgttagat ttaaatattg gatactcctc tatttccaaa
     1741 atagaacgca tcaaatatag cgattattat tattcacaac aagtagggtt agacgacgat
     1801 gatctcgccg aattagaaaa aatcgataat agactagact tctttcctgt acttaatgaa
     1861 gcacttgaat actctagcaa tttttatagc gacagttttg attattatga cccatatcgt
     1921 tatggcatca atactattgc acacctaaaa gacgcagatt taacaaaagc taatctcagc
     1981 ttattagcgg gcacattacc gaccaataaa aacgaagttg cggttcccga atatgttttt
     2041 gattccttta aaaagtatgg atatcgctca ctgactaata gcactaaaat tgcaattgat
     2101 gaccccgaag atttgcttaa taaaacattg aacaataaat ttgtaatttc tggtgtatat
     2161 aaaactggtt tagaagttga ccgtttcata attgagaatg ttgatgatga tggtagtatt
     2221 tataacacga ttaacactga agtttttaga caatatttat taaatgggtt tcataccaca
     2281 ctacttgtgc atgaagagta ctacaataaa cttgttgaac aaaataaggt atattctttg
     2341 ccctttaact ctaattctta tttccaaatg ggagatgttg atcgttatca atttacaacc
     2401 cgtaacttcc gcagaattga aagtttaaaa actaacaacg atgttatttt atttaatggc
     2461 ataacgcacg ataatttagg tcgtgatgat attatactta gcacttctgc cgctttcttc
     2521 caaggtatgg aaggcggtgt ccatgattca gttcaaaccg cacttttagt atttgtcgat
     2581 aatataaagt caacagatga aaacgacctt gcgattgagc aaatgttaaa aactaattat
     2641 caacattatg gttttaatag cccccttgat gttactgcac catctctctg gagtgaacaa
     2701 gaaaaaacaa gcttccggaa tttttatgcc caagcactac tcaccttcct ctatgatgaa
     2761 aacccgttcc aacccgaact cattggtcgc gaagccatct ttgctgtaac tagcgcacaa
     2821 attaaaactg actttgcgcc atttaacatt gaatatttcc ttgttgcata tgaaagtgat
     2881 ggagaaaagc taaaagcagc gcaggattta cgtgttgttg gttttacttt tgactatcag
     2941 tattatattg aagcgtggga gagtattatt atttatatta atgaagaaaa tgctgaagtt
     3001 atgaatttaa cacctgaagg tgcttacttc cgtgcagtta cttatcacgg caattcacgt
     3061 gctattattc aaaaacttgt taagtttaat gaactaggaa caaaaaagga tgttggttct
     3121 ttttatcgca tgaataatga ggtttcttat cttgttgaaa atgttggaga cttcttagaa
     3181 attatttcta gaatatttgt cgttgttggc attatcttcg cggtatttgc ctcactactc
     3241 ttccttaact tcattacact aagtgtggca tttaagaagc aagaaattgg gatactacgt
     3301 gcgattggtg cacgtgggat tgatgttgct actatcttta tgaacgaagc aggaattatt
     3361 gctttaatta atctcatact ctcatttatg cttgccatca tcgctggaac aacaattaat
     3421 aattacattg ggaaaaatat taatatttat ttatcaattg tcaatgttgg cctaaggcaa
     3481 ttattacttc taattctcat tgcctttggc gtagcgttta tttctagctt tattcctgtc
     3541 ttccgcctta gtcgcaagaa accaatcgac gcaattagaa tgcgctagaa gtatttatgt
     3601 gacattaaaa aatgcgcttc ttacaagcgc attttcttta atgttcacaa tctttattta
     3661 attggtgata tgtaaaattt accaaaaact tgtcgcaagt catagacatt aataaaggct
     3721 ttaggatcga cttgttgtgt taagcgcact acgtcattta cttcatttga tgaaactgtc
     3781 atataaataa cagatttttc actttgtgaa tacacaccaa tacctttaat tgttgtcgca
     3841 ccatgcggga agttggaaat aagaattttc gcgagttctt catcatttgt aataatttgc
     3901 acttgcgatt ttttattccg cacattaatt aagtcaatta caaatgaatt cattacaaga
     3961 taagtaacac tagaaataaa acttgtaatc gctacattag tgttcgtaaa ttgtgtatta
     4021 gcgatatcaa tattagtgct cggatgaatt aagttcagga caaaataaaa accaacgact
     4081 acgacattaa agagcacatt atatttacca acaccttcgc ttttcttatt cgcaaagtag
     4141 taggcaataa tatccattcc gccactacta acattaccac taaaagcaac cgcagcgcta
     4201 aaaccagcaa ggatgcccgc gtaaagtgta cgcgttaatt gcgagtcgta aattaccgcc
     4261 ttctcccaac taactggaat aatactaata aataatgatg caagtaaaac atttataatc
     4321 gaaaaaatag taaatctccg accaatttta agccacccta aaataaaaac gggaatattt
     4381 aacgtaaagt acaaaattga ttgtaaaacg cgaggattaa ctttttgcca accgataata
     4441 tcatgaatta atttaactaa attttggctt aaaccagaaa caccgccccc cacaaacaca
     4501 atttgattac cactgacact gtggagcgtt tcaagcgatg gactaataaa taaacgaaat
     4561 gctaaagcat aagtaaacga actaaagagc actaacatta aaagatacat ccactcggtt
     4621 gcaaccttta agcgtggtcg gaatgaaagc cactctctaa ttttactttt gacactaaaa
     4681 cgaataccca taattacctc actatcttga attataggtt taatttaata aacttgcaaa
     4741 atataactat ttaaaactat tgttttaatg aaaaaacaca ttgttttgct attagatttc
     4801 agaaacttta gtaaattgct ttttttatta ttgcttttgt ttgaaaatat atgttgag
//
LOCUS       DAOSLP010000058         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_16220_length_4694_cov_8.769131, whole genome shotgun sequence.
ACCESSION   DAOSLP010000058 DAOSLP010000000
VERSION     DAOSLP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16220_length_4694_cov_8.769131"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..98
                     /locus_tag="PK460_04760"
     tRNA            22..98
                     /locus_tag="PK460_04760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56..58,aa:Arg,seq:tcg)
     gene            157..1713
                     /locus_tag="PK460_04765"
     CDS             157..1713
                     /locus_tag="PK460_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54417.1"
                     /translation="MKKIFKISLLLVATLLSACRSSSSSEHSEKLVSSSSEKTSEITS
                     SSSESSSSTAFRILDTVKNICIENNYLKWDAVTEATHYNVMVGSTGAEYERKTVYDTN
                     ELDLMTLSKVYTCATVVTITAHNSHAISVPSDAFTIICIDGVYYEFYSSPFEITYTNF
                     KVTDYELSWETNANFTEIKVGSDDPRVIPQTYISLPDYISGNAIHEFTFKGRVLLKEG
                     PSFTVKVISHDLKYYLYDDNLTLETPTLVRDDNIVTIIANGLYNSFEVSIGTHNFTTT
                     ASNFDLLDYAEYMSGEAFTVSVKAAYNDILYSEAATLSLVYIDGVIYNSGEEPSITGF
                     ALKNGKVLLWDKVEGISEYDVQIGEYKKTVSTNEISLYNERNHIKRHTPVVIKAGNLS
                     GVCHINCDYETLSFSIEAVPKLNTPQGFNIEEKMYLTFARVPNDTANSLGMGSSYYLY
                     YNDGYSYETSSPAGASNTVSFNLGYISGWYTNPQVVRIVVHGHSDWASSEAALVTINR
                     LNHDHYEIIY"
     gene            1798..2673
                     /locus_tag="PK460_04770"
     CDS             1798..2673
                     /locus_tag="PK460_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE54418.1"
                     /translation="MNLAAQLREVLNNWFASFIANESLAQILVTAIMIIFWVILAYLV
                     TLVIKLIIFRTRRVERKLGVKETKEKQTVRRLINSLIRFFFLFWIIIMILKELGLDLV
                     PLLAGAGVVAFAVGFGAQELIKDIIAGMFLILEHTFKIGDFVEIGGQSGTVVDVGLRR
                     LKIQNWKGEVITMNNGDIKVVMNASINPSIAIIEFKMDYPFNVKELETADFRAFLKKF
                     KDKNPQVLEMPEKIVLMDVNDGLLFRVTIKTESRQHIGIEREFRKELYKYVQAKNWPI
                     IVPVVVEEHDSSYQA"
     gene            complement(2739..3530)
                     /locus_tag="PK460_04775"
     CDS             complement(2739..3530)
                     /locus_tag="PK460_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54419.1"
                     /translation="MEKSLQPTDTKKRRRRKRPRRIRQNWFFRFLRNFVKLFKKRPLI
                     YDHNEDIVGPAIFVSNHSGAAGPMDLSMFFPYYFVPWGAHEMRGNYRTRWKYLYYVFY
                     QQKLHYGKFKSFFLATILAVINPFLYANMRLIPTYQDIRFTKSLRESLTYLDDGIPIL
                     VFPEDSSEGYFDTIIKFNEGVVVLADYVDKHRNLNIPIYPVYYSKRAREIEIGKPAYY
                     RDLKAKGHTRSEIADILRKHINTLYENIKARKARNAKAKERVRKG"
     gene            3619..4632
                     /locus_tag="PK460_04780"
     CDS             3619..4632
                     /locus_tag="PK460_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOE54420.1"
                     /translation="MYQIRAKRKYSNILIAPFGRRMKAALLDLLLVLVLGFFSFMAVD
                     AIYTNSKVGSTANLTFYNIKKDSGLYHSYDESRTTAFLNLDINERDNYVRYFLGLEYF
                     YTQENTGFIYEKSVYYTEDTEFNYRTMVLKEGEDDSFFDFDTPYKTISYSFKANLSAG
                     DRNEVWKNLYANALTDLMRTPAYQKAEKVLKQFIVLNLSISAFSGTILPILAAPFFFG
                     HGRTLGKFLTGLAVVDKNGYKVSWWRMLIRSLLYGVVEIGLNFYGFFMPLLLTSGAVA
                     LTHKRQALHGIFSGTYVVDARTSKIFNNINDEEEYFSLSPEERELNKNYYQEKPYVPL
                     KKL"
BASE COUNT         1533 a          792 c          859 g         1510 t
ORIGIN      
        1 tttagattaa tctaaatatg tgtcccggta gttcagttgg atagagcaac agtcttcgaa
       61 actgtaagtc gggcgttcga gtcgctcccg ggacgccacg ctaaatataa tgcttataaa
      121 agcaaggtat ataaatttaa agaaggggta aacataatga aaaaaatatt taaaatatca
      181 ctattactcg tagcgacatt actttctgca tgtcgctctt cttcaagcag tgaacatagt
      241 gaaaaattag tctcttctag tagtgaaaaa actagtgaaa ttacttcatc atcttctgaa
      301 tcttcttcat caacggcatt tcgcattctt gatacggtaa aaaacatttg cattgaaaat
      361 aattatttaa agtgggacgc tgtaactgaa gcaactcatt acaatgtgat ggttggttct
      421 actggtgcgg aatacgaacg aaaaacagta tacgatacca atgaattaga tttaatgact
      481 ctatcaaaag tgtatacttg cgctactgtg gtaacgatta ccgcgcataa tagtcacgct
      541 atcagtgtcc caagtgacgc ctttacaata atttgtatcg atggtgtata ttatgaattt
      601 tattcatccc catttgaaat aacatacacg aattttaaag tgactgatta cgaactttcg
      661 tgggagacaa acgccaattt taccgaaata aaagttggca gtgatgatcc gcgcgttatt
      721 ccgcaaactt atattagtct tcctgattac ataagtggga acgctattca tgaatttaca
      781 tttaaaggcc gtgtcctttt aaaagaaggg ccatcattta ctgttaaagt aataagtcat
      841 gatcttaaat attacttgta tgatgataat ttaacgctag aaacacctac tttagttcgt
      901 gatgataata tagtcacaat aatcgctaat ggcttatata atagctttga agtttctatt
      961 ggcactcaca atttcacaac aacagcatca aattttgatt tactagatta tgctgaatat
     1021 atgagtggcg aagcatttac tgttagtgtt aaagcagcgt ataacgacat tctttatagt
     1081 gaagcggcaa cactttcact agtgtatatt gatggcgtaa tttataactc tggagaagag
     1141 ccaagtatta ctggttttgc cttaaaaaac gggaaagtac tcttgtggga taaggttgaa
     1201 ggcattagtg aatatgatgt gcaaattggt gaatataaaa agactgtttc cacgaatgaa
     1261 ataagtttat ataatgagcg aaatcacatt aagagacata cgccggttgt tataaaagca
     1321 ggtaatttaa gtggtgtttg tcatattaat tgcgattatg aaacgctaag tttttcaatt
     1381 gaagcagtcc ctaaattaaa tacaccacaa ggttttaata ttgaagaaaa aatgtatctt
     1441 acatttgcaa gagtaccaaa tgataccgct aattcgcttg gcatgggttc ttcatattac
     1501 ctttactaca atgatggtta tagttatgaa acttcttcgc cagcaggtgc aagtaacact
     1561 gtttctttta atcttgggta tatttcggga tggtacacta atccgcaggt agtgcgaatt
     1621 gtggtgcatg gtcatagcga ttgggcaagc agtgaagcgg cactagtgac gattaatcgc
     1681 cttaatcacg accattatga aatcatctat taattaaact tttaatttta taacataaca
     1741 ttggaaaaaa gtggcatatt tcgctaaaat tacaagcgtt aatggaggta tatcaatatg
     1801 aatttagcag cacaattacg tgaagtatta aataactggt tcgcgtcttt tatcgcaaat
     1861 gaatcacttg cgcaaatttt agtaacagca attatgatta tcttttgggt gatccttgct
     1921 tatttagtta cgcttgttat taaattaatt attttccgca cacgccgcgt tgaacgtaaa
     1981 cttggggtta aagaaacaaa agaaaaacaa acagtgcgtc gccttatcaa tagcctaatc
     2041 cgtttcttct ttttattctg gattattatt atgatcttaa aagaattagg acttgattta
     2101 gtgccccttc tagcaggtgc tggggttgtg gcttttgccg ttggctttgg tgcccaagaa
     2161 ttaattaaag acataatcgc ggggatgttc cttattttag aacatacttt taaaattggt
     2221 gacttcgttg aaatcggtgg tcaatccggc actgtcgtag atgttggttt acgacgcctt
     2281 aaaattcaaa actggaaagg tgaagtaatt acgatgaata atggtgatat taaagtagtg
     2341 atgaacgcta gtattaaccc aagcatcgcc atcattgagt ttaaaatgga ttatccattt
     2401 aatgttaaag aattagaaac agcggacttt agagccttct taaagaagtt taaagacaaa
     2461 aacccacaag tacttgaaat gcctgaaaaa attgtcttaa tggatgtaaa tgatggttta
     2521 ctctttagag ttacaatcaa aacagaatca cggcaacaca tcggcattga acgcgaattc
     2581 cgtaaagaac tttataaata tgtacaagct aaaaattggc caattattgt gccagttgtt
     2641 gtcgaagaac acgattcaag ttatcaagct taaactattt aaaagcaaag taaaaaagcg
     2701 gcaatctagc cgccttttgt cgttataata agagcatttt aaccttttct tactcgttcc
     2761 tttgctttcg cgtttcgtgc ttttcttgct ttaatatttt cgtataatgt attaatatgc
     2821 ttccgtaaga tatctgcgat ttcagagcgg gtatgaccct ttgcttttaa gtcacgatag
     2881 taagcgggtt taccaatttc aatctcacgc gcccttttac tgtaataaac cggataaatt
     2941 ggaatgttaa gattacgatg tttatcgaca tagtcagcaa gcacgacgac accttcatta
     3001 aatttaataa tcgtgtcaaa ataaccttca cttgaatctt cagggaatac aagaatagga
     3061 atgccgtcat ctaaataagt taaactttcc cgtaaactct tcgtaaaacg aatgtcttga
     3121 taagtaggaa ttaggcgcat atttgcataa aggaaaggat taattaccgc aagaattgtc
     3181 gctaagaaaa aacttttaaa tttaccgtaa tgtaattttt gttgataaaa aacataataa
     3241 agatatttcc agcgtgtacg atagttacca cgcatctcgt gcgctcccca tggcacaaag
     3301 taataaggga aaaacattga aagatccatt ggacccgctg caccgctatg gttactaaca
     3361 aaaatagcag gaccaacaat atcttcatta tggtcataaa taagcggacg cttcttaaat
     3421 agcttcacaa aattacgtaa aaaacggaag aaccagtttt gcctaattct tcgtggtctt
     3481 tttctgcgcc gtcttttttt tgtatcagtc ggttgtaaac ttttttccat attgttcatt
     3541 ttaccgaata agcgtacttt tagcaaagga agataccaaa tcttaaactt agtgcggcat
     3601 atttgctata atatcgttat gtatcaaatt cgcgctaaac ggaaatattc aaatatatta
     3661 attgccccct tcgggcgcag gatgaaagct gcacttttag acctcttatt agtgttagtg
     3721 ctcggctttt ttagttttat ggcagtggac gctatttaca cgaatagtaa agtaggaagc
     3781 accgctaatt taacttttta taacattaaa aaagattcag gtttatatca tagttatgat
     3841 gaatcgcgga cgacagcatt tttaaattta gatattaatg agcgtgataa ttatgttcgt
     3901 tacttcttag gtttagaata tttctatacc caagaaaata ctggtttcat ctatgaaaaa
     3961 tcagtgtatt atacagaaga caccgaattt aactatcgca caatggtatt aaaagaagga
     4021 gaggatgact cattctttga ttttgataca ccatataaaa caatctctta ttcatttaaa
     4081 gctaatcttt cagctggtga tagaaacgag gtgtggaaaa atctctacgc gaacgcttta
     4141 actgatttaa tgcgtactcc cgcttatcaa aaagccgaaa aagttttaaa gcaattcatt
     4201 gttttaaatt tatcaatcag tgccttcagc ggcacaatat tacctattct tgcagcgcct
     4261 ttcttttttg ggcacggacg aacgcttggt aaatttttga ctggacttgc tgttgttgat
     4321 aaaaacggct ataaagtatc gtggtggcgt atgttaattc gctctttgct atatggcgtt
     4381 gtcgaaatcg gcttaaattt ctatggcttt tttatgccgc ttttactcac aagcggagcg
     4441 gttgcactta cacataaaag acaagcgctg cacggtattt tcagtggcac atatgtcgtt
     4501 gatgcaagaa cctcaaaaat atttaataac attaatgacg aagaagaata tttttcacta
     4561 agtcctgaag agcgtgaact taataaaaat tattatcaag aaaaacccta cgttcccctt
     4621 aaaaaacttt aataaacatt acttttaatt aataaaaaag tcccagatgc gtgggacatt
     4681 ttcattttga ctgg
//
LOCUS       DAOSLP010000059         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_20051_length_3972_cov_6.790656, whole genome shotgun sequence.
ACCESSION   DAOSLP010000059 DAOSLP010000000
VERSION     DAOSLP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20051_length_3972_cov_6.790656"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..646)
                     /locus_tag="PK460_04785"
     CDS             complement(71..646)
                     /locus_tag="PK460_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54421.1"
                     /translation="MNVKVVYPAKNIRHDIRRAAIYWAKPAFILAAIISAVVNILVKG
                     SAWSVIVIWSLWMIWSFVFTPTLIEHNRTSIAVKSSIHVTILIVIIYMIYPSWPGIEV
                     ASLVVVGGLIITAILFFSNVSRQKQNIFPLLIFIIIAIIFSVVAFVLRGQEPLGWAMI
                     VSISVALAIFLSTAIILRINFFRELKKRFML"
     gene            complement(609..2129)
                     /locus_tag="PK460_04790"
     CDS             complement(609..2129)
                     /locus_tag="PK460_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54422.1"
                     /translation="MRKRKINEFTEYLNHLNADDVQKVKAFMVNFKQHMNLPLSEEKK
                     LRKDLKGALMYYNAIGVSVTEALRRLDNENLGGFYARPPLLYYALDDAAKIYPLSMKH
                     GFQAVFRLSANLYDEVVPQLLQIALTFTIKRFPRFATTLKKGFFWHYLDANKQRYGIE
                     KEEHLPCRPIKVATTGSKSFRVLYYKNRISVEFFHSLTDGKGGIVFLQTLLDTYLRLF
                     GVEGHKHPSVLDINAAPLPEEDANEFKRTTVTKRSGFKEKRALQMSGKIESIRPTQIL
                     HFRLDAKELAKTAKSYDVTITTYVLALILLASRFATDAKEGDIAIQVPVDLRKYYESK
                     TLANFSLYGGIRIPITSITTMEQLLPLVKEQLVTATSHEKMSEMMYSAHRMNKDVRGF
                     PLFLKSPIAKLAYGLMGEAIFTTTLSNLGVVSAPDYFSDYIESYDFVLGPPEVNRAAL
                     TLITYNNIATLTITKSTKDPSFEEEIHRLLTKDGLDITIEGSAVYERKSRVSRKKY"
     gene            complement(2188..2538)
                     /locus_tag="PK460_04795"
     CDS             complement(2188..2538)
                     /locus_tag="PK460_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54423.1"
                     /translation="MQILFIVLNDLSYLDKILEKFLDLHVRGATIIDSQGMAAAIMQN
                     EGLLSLLSGPFARSLETNQKNSKTIFTVIPEAEKVTEVVKAVQDIISDCKKQVVGFMF
                     TLPVSGIYPLKPKK"
     gene            complement(2541..3887)
                     /locus_tag="PK460_04800"
     CDS             complement(2541..3887)
                     /locus_tag="PK460_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE54424.1"
                     /translation="MDAIFVLILKLAIVLSVGFIGSLVARKLKLPNVSGYLLFGLLLG
                     PSLGLIFKGFPGIITVEDSGNLNFISQIALAFIAFSIGAEFSIKSIKKVGKPILVMTS
                     TEVLGAVLVVFFAMFFLPKPQAIMPDGYNPFSKPNVAFGLILASMSAATAPAATLMVI
                     RQYRAFGPVTRAILPITALDDIFGIVVFGFFISFAQILASTGPLPPVWLMILKPFIEV
                     FGSVALGMIIGLLLSLLANKFDRIRDDLQVLSLIAVFGTIGLTYLINYYLEPHGLSFS
                     QLLSNIMIGSMIANIAKRPEKTFNAINDFATPFYILFFTLAGAGLDLAILGTTPLLLL
                     IAGVYILARGFGKWLGIFVGANVVKAEPTVKKYLGIALLPQGGVSIGLLAIVYTQMAP
                     MYPAISTVIMLSVLVYETMGPVFAKFAISKAGEIGGLDRLEQLSSLDGIELTEEGA"
BASE COUNT         1281 a          756 c          763 g         1172 t
ORIGIN      
        1 aacccgcacg gttagcttgg gaagctaaag ttctaccact aaactacagc tgcgtttttt
       61 ctacaataaa ctataacata aatcgctttt ttagttcacg gaaaaagtta attcttaaaa
      121 taattgccgt acttaaaaat atagcaagtg cgacactgat agaaacaatc atcgcccagc
      181 caagtggttc ttgaccgcgt aatacaaagg ccacgacact aaaaataata gcgataataa
      241 taaagattaa aagcggaaag atattttgct tttgacgtga gacattacta aaaaataata
      301 tagcggtaat aattaaacca ccaacgacca cgagtgaagc aacttcgatt cccggccaac
      361 ttggataaat catataaatt atgacgataa gaatagtgac atgaatcgaa gacttcacgg
      421 cgatacttgt gcgattgtgt tcaataagtg ttggagtaaa cacaaatgac catatcatcc
      481 ataagctcca tatgacaatc acgctccagg ccgagccttt cactaagata ttgacaacag
      541 cgctgataat tgccgctaaa atgaaggcag gctttgccca gtaaatagcg gcacgacgaa
      601 tgtcatgtct aatatttttt gcgggataca cgacttttac gttcatatac agcactccct
      661 tctatcgtta tgtctaaacc atcttttgta agcagacgat gtatttcttc ctcgaaggat
      721 ggatctttcg tacttttcgt aattgttaaa gtagcgatat tattataagt gattaatgtt
      781 aatgctgcgc gattaacttc aggtggacca agcacaaagt cataactttc aatatagtca
      841 ctaaaataat ctggggcact tacaacccct aaattactta atgttgtcgt aaagattgct
      901 tcacccatca gtccataggc aagtttagca atcggtgact ttaagaaaag cgggaaaccg
      961 cggacatctt tattcatccg gtgtgcggaa tacatcatct cactcatttt ttcatgactt
     1021 gtggccgtta ctaattgttc cttgacaagt ggtaaaagtt gttccattgt agtaatactt
     1081 gtaattggaa ttcttatccc accataaagc gaaaaattag cgagagtttt agactcataa
     1141 tatttgcgta aatcaacagg tacttgaatt gcaatatcac cttccttagc gtctgttgca
     1201 aagcgacttg ctaaaaggat tagcgctaag acataagttg taatcgtgac atcatatgac
     1261 ttggcagttt tagctagttc tttcgcatca agtctaaaat gcaatatttg tgtcgggcga
     1321 atgctttcaa ttttaccact catttgtaag gcccgttttt ctttaaatcc gctgcgcttt
     1381 gtgacagttg ttcttttaaa ttcgtttgca tcttcttcag gaagtggtgc ggcattaata
     1441 tcgagtacac ttggatgctt gtgtccttca acaccaaaaa gacgtaaata agtatcaagt
     1501 agtgtttgta aaaagacgat tccgcccttt ccgtctgtta acgaatggaa aaattcaaca
     1561 ctaatccgat ttttataata taaaacgcgg aatgattttg aacctgttgt agcgacttta
     1621 atcggacgac atggtaaatg ttcttccttt tcaataccgt agcgttgctt attagcgtct
     1681 aaatagtgcc agaagaaacc tttttttaag gttgtggcaa agcgcgggaa gcgtttaatc
     1741 gtgaaggtaa gcgcaatttg taaaagttgc ggtacaactt cgtcatatag atttgcagat
     1801 agtcgaaaca cagcttgaaa gccgtgtttc attgataaag gataaatctt tgcggcgtca
     1861 tcaagcgcgt aatataaaag agggggacgt gcgtaaaatc ccccaaggtt ttcattatca
     1921 aggcggcgca gagcttcagt tactgataca ccaatagcgt tgtaatacat cagagcccct
     1981 ttaaggtctt tccgtaattt cttttcttcg ctaagtggta agttcatatg ttgtttaaag
     2041 ttcaccataa aagctttcac tttttggaca tcatcagcat ttaagtgatt taaatattcg
     2101 gtaaactcat ttattttgcg ttttctcata atgatagtaa ttataacacg attatgtaat
     2161 cgtgaattta ttgatgttta gatgatttta ttttttaggc ttaagtggat aaataccgct
     2221 tactggcaag gtaaacataa agcccacaac ttgtttttta caatcagaaa taatgtcttg
     2281 gacggctttt acaacttccg tcactttttc tgcttctgga ataactgtaa agattgtttt
     2341 tgagtttttt tggttagttt caagtgatct cgcaaaagga ccggagagca atgagagtaa
     2401 gccttcattt tgcataattg ctgcggccat tccttgtgag tcgataattg tcgcaccgcg
     2461 cacatggaga tctagaaact tttctaagat cttgtctaag taagataagt catttaaaac
     2521 aataaataag atttgcataa ttatgctcct tcctcggtta attcaatacc gtcgaggctt
     2581 gataattgtt caaggcgatc aagacctcca atttcgccgg ctttactaat ggcaaatttt
     2641 gcaaagactg gacccatcgt ttcatacact aatactgata acattataac agtagaaatg
     2701 gcaggataca tcggggccat ctgtgtgtag acaattgcca gtaatccaat tgagacacca
     2761 ccttgtggta aaagggcaat gcctaagtac tttttcaccg ttggttcagc tttaaccaca
     2821 ttagcgccta caaagatacc aagccactta ccaaagccgc gcgctaggat atagacacca
     2881 gcgattaata gtaagagcgg ggttgttcct aaaatggcta aatcaaggcc tgcaccagca
     2941 agggtgaaga aaagaatata aaatggggta gcaaagtcgt taatcgcatt aaatgtcttt
     3001 tcgggacgtt tagcaatatt agcgatcatt gacccaatca taatgtttga taataattga
     3061 ctaaatgata aaccgtgtgg ttctaagtaa taattaatca aatatgttaa tccgattgta
     3121 ccaaagacag caataagtga taagacttgc aagtcatcac gaatgcggtc aaacttattc
     3181 gctagaagcg ataagagtaa accgattatc attccaaggg caaccgagcc aaacacttca
     3241 ataaatggct ttaaaatcat gagccacact gggggtaaag gaccagttga cgctaagatt
     3301 tgcgcaaaag aaataaagaa cccaaagaca acaataccaa agatatcatc aagcgcggta
     3361 attggtaaaa tagcgcgtgt cacaggacca aaagcccgat attgccgaat aaccataagg
     3421 gtagcggctg gggcagtcgc cgcactcatt gacgctaaaa ttaaaccaaa ggccacatta
     3481 ggtttgctaa atgggttata accatcaggc ataattgctt gtggttttgg taaaaagaac
     3541 attgcaaaga atacgactaa taccgcacct aatacttcag ttgaagtcat aactaaaatt
     3601 ggtttaccga cctttttaat tgatttgata ctaaattccg caccaattga aaaagcgata
     3661 aacgcaaggg cgatttggct tataaagttg agattgccac tatcttcgac agtgataatg
     3721 cccggaaagc ctttgaaaat aagaccgagt gatggtccaa gtaacaaacc gaaaagtaaa
     3781 taaccgctaa cattcggtaa ttttaatttt cgggccacga gtgatccgat aaatcccacg
     3841 gatagtacga tggcaagttt taggattaaa acaaaaattg catccataat tttctcctct
     3901 cataaaaata attaatgttc ttaattactc ctatgaggtt agctgtcggg ttcaggacat
     3961 taagaatatc ct
//
LOCUS       DAOSLP010000060         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_20076_length_3967_cov_12.707055, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000060 DAOSLP010000000
VERSION     DAOSLP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20076_length_3967_cov_12.707055"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            807..1010
                     /gene="rpmE"
                     /locus_tag="PK460_04805"
     CDS             807..1010
                     /gene="rpmE"
                     /locus_tag="PK460_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOE54425.1"
                     /translation="MKQGIHPKTQKITVTCVSCGTEFEALSTKKEIRVDTCSNCHPFY
                     TGKQRFTQADGRVDKFNKKYGLK"
     gene            1071..2651
                     /gene="metG"
                     /locus_tag="PK460_04810"
     CDS             1071..2651
                     /gene="metG"
                     /locus_tag="PK460_04810"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOE54426.1"
                     /translation="MKKRFYITTPIYYPSAKLHIGHAYCTTLSDILARYNRMIGKEVY
                     FLTGSDEHGQKIAENAAKANKTPQKFVDDIVKTFKDLWEKLAITNDDFIRTTDPKHIA
                     TVQKIFTKLLEQGDIYLGSYEGWYCTPCESFWTTTQVGEEKLCPDCNRSVHLASEESY
                     FFKTDKYVEGLLKKYEETPNFITPESRKNEMVNTFIKPGLSELAVSRTSIEWGVPVRE
                     NERHTMYVWVDALINYISALGYLSEDDELFHKFWQSEDVEIVQILGADITRFHTIYWP
                     MILKALGLRQADRYFVHGLLMMKDGKMSKSKGNVIDPLPLIDKYGVDAVRYYLAREVV
                     FGNDGQFTPEQFVERFNVDLVNDFGNLLNRTVSMIEKYCDGVIPLYRGQVTEFDTEIE
                     DLAINVVKEYHEHFSDLKVTEAITTAFKIISASNKYIDNTKPWELNTQGKKEELDSVM
                     NHLANNLFIGAILLQPVLVNAPKELFKQLGVPQDKQDINLLNKYGVLGGEKVNKGEPL
                     FPRLKVNEEVEYIKNLMK"
     gene            2651..>3967
                     /gene="rlmD"
                     /locus_tag="PK460_04815"
     CDS             2651..>3967
                     /gene="rlmD"
                     /locus_tag="PK460_04815"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOE54427.1"
                     /translation="MIKENDIYVVTGGDYTSEGIGLAKVDNFPIFINNLIIGEEAEIK
                     ITKVMRNFATGKIIRFTKMSSDRITPVVPETIHLAGCQFTQLDYAKEVEYKLSKVERA
                     LRVIGGIDFKIEKIHTAKNPYFYRNKTVLPFGKRKSGKVYTGLFRHNTHEIIAMEKTH
                     LDDELASIVIAEVRKLIEKYNYEVYDEKSHTGQVRKVMVRVSHYLREVMVVLITKDEQ
                     IPNLDAFCRDLVAICPNITTIIQNINSEDTNVILGKKEKVLYGPGFINEELHGLKFTL
                     SSKSFFQVNTLQAEVLYERALTLAGITKNDVVLDAYSGTGTIAILAAKKAKQVIGVEI
                     VKDAVKDAEVNAKQNNITNVKFFKDDAGAFLTREIKNYNFSVVIVDPPRKGLSDEFVN
                     TLLRALPPKIMYVSCDPATLARDLKKLTKKYNVEHIECVDMFPRTAH"
BASE COUNT         1465 a          584 c          740 g         1178 t
ORIGIN      
        1 cctcctaatt aaaaatgata acaaaaatat gaaggaaata tggggaaaag cgctttttta
       61 gggaatttta gataatacaa caatttaata ttcaaattgt aacgaataag ggcagctact
      121 aataaaaaag attaatattt ctttaaaaag cactaaaaaa atcacgctaa attatatgag
      181 caacaaaatt aacaattatt ggttttgttg taacttaacg aaaggaggag aaatattaat
      241 gaagaataaa aaaacataaa tgaattaagt tatgaagcaa aaaaagttta tgataaaagt
      301 caattttcaa aaaaagcatg taaaagcaac attgctttct atgtttttga ttgcaataat
      361 cgttcaaatt caaaaaatct aactttgagt aatttcatta ttataattaa cgagttgatt
      421 gaagcgcagc gatacagtgc ataccatata gaagaaacat caaaaaatta ctatttcata
      481 acattcattt aaaagaagaa aaaagagttg tattgatgtt gatgttgatg tgtacccctt
      541 ttttgttatc aaagatttgg ggtacacttc acttcaaaaa aagattgtat tgtttactat
      601 gatattaaaa tgcaactatc tgttgcaagt aataatttta ctagggtact tgttttggga
      661 actttttatt gatttcataa aatatcataa aagagattat tagaactcag gaataatatt
      721 ttcggttgtc aaataactgt aataatgata ttattacttt tgcttactta ggttaaatcg
      781 agttaaccta ggcggaagga gatattatga aacaaggaat tcacccaaag acgcaaaaaa
      841 tcacagtgac ttgtgttagc tgtggtactg aatttgaagc attatcaaca aaaaaagaaa
      901 tccgtgttga cacttgctca aattgccatc cgttttatac agggaaacaa cgctttaccc
      961 aagctgatgg tcgtgttgat aaatttaata aaaaatacgg acttaagtaa attgattact
     1021 gaaaaactgt taaatcaaat ggcagttttt tcaaatataa gaggtacact atgaaaaaac
     1081 gtttttacat aacaaccccc atttattatc caagcgctaa gttacatatt ggtcacgctt
     1141 actgtacaac tttaagtgac attttagcgc gctataatcg gatgattggt aaagaagttt
     1201 atttcttaac tggaagtgat gaacacgggc aaaaaatagc ggaaaacgcg gcaaaagcaa
     1261 ataaaacgcc acaaaagttc gtggatgata ttgtaaaaac atttaaagac ctctgggaaa
     1321 aattagcaat aacaaatgat gattttattc gcacgactga tccaaaacac attgcaactg
     1381 tgcagaagat tttcaccaaa cttttagaac aaggtgatat atatcttggg agttacgaag
     1441 ggtggtactg tactccgtgt gaatcatttt ggactacaac tcaggttggt gaagaaaaac
     1501 tttgtcccga ttgtaatcgg agcgtgcatt tagcaagtga agagtcatat ttctttaaaa
     1561 ccgataaata tgttgaagga ttattaaaga aatatgaaga aacgccaaac ttcattacgc
     1621 ctgaatcgcg taaaaatgaa atggttaata catttattaa accaggacta agcgaacttg
     1681 ccgtctcacg cacgagtatt gagtggggag tgcctgttag agaaaatgag cgccacacaa
     1741 tgtatgtgtg ggttgacgct ttaattaatt atatttcggc attagggtat ttaagtgaag
     1801 atgatgaact cttccataaa ttttggcaaa gtgaagatgt tgaaatagtg caaatcttag
     1861 gtgccgatat cacgcgcttc cacacgattt attggccaat gattcttaaa gcactagggt
     1921 tacgtcaagc agaccgttat tttgtccatg ggctactgat gatgaaagac ggaaagatgt
     1981 ctaaatcaaa aggaaatgtt attgatccgt tgccgcttat tgataaatat ggcgtggacg
     2041 ctgtgcggta ttacttagca agagaagttg tgtttggtaa tgacggacaa tttacacctg
     2101 aacaattcgt cgaacgcttt aatgttgatt tagtaaatga cttcggcaat ttattaaata
     2161 gaacagtatc gatgattgaa aagtattgtg atggtgttat tccgctttat agaggtcaag
     2221 taactgaatt tgacaccgaa atcgaagact tagcgattaa tgtcgttaaa gaatatcacg
     2281 aacactttag tgatttaaaa gtgacagaag cgattaccac tgcttttaaa attattagtg
     2341 catcaaacaa atacattgat aacactaaac cgtgggaatt aaacactcaa ggtaaaaaag
     2401 aagaattaga ttcagtaatg aaccacttag caaataacct ttttattggc gcaatattac
     2461 tgcaacctgt actagtaaac gcaccaaaag aattatttaa gcaacttggg gtgccgcaag
     2521 ataaacaaga tattaatcta ttaaataaat atggtgtttt aggtggagaa aaagttaata
     2581 agggcgaacc attattccca cgcctaaaag ttaatgaaga agtcgaatac attaaaaact
     2641 taatgaaata atgattaaag aaaatgacat ttatgtcgta actgggggcg actacactag
     2701 tgaagggatt ggcctagcta aagtcgataa ttttcctatt ttcattaata acttaattat
     2761 tggtgaagaa gcggaaatta aaataacgaa ggtaatgcgt aattttgcga ctggtaaaat
     2821 aattcgtttt actaaaatgt ctagtgatcg tattacacca gttgtcccgg aaacaatcca
     2881 tcttgcaggt tgtcaattta cccaacttga ttacgcaaaa gaagtggaat ataaactttc
     2941 aaaagttgaa cgagccttac gcgttatcgg tggcattgat tttaaaattg aaaagatcca
     3001 cactgctaag aacccatatt tctatcgtaa taaaacagta ttaccgtttg gcaaaagaaa
     3061 gagcggcaaa gtttataccg gtttatttag acataacacg cacgaaataa ttgcgatgga
     3121 gaaaacacat cttgatgacg aattagcaag catagttatt gctgaagttc gtaaattaat
     3181 tgagaaatat aattacgaag tttatgacga aaaaagtcat actgggcaag ttagaaaagt
     3241 aatggttcgt gtaagtcatt atcttcgtga agtaatggtt gtacttatta caaaagatga
     3301 gcaaattcca aatctagacg cattttgccg tgatttagtg gcaatttgcc caaatattac
     3361 aacgattatt caaaatatca atagcgaaga taccaatgtt attttaggta aaaaggaaaa
     3421 agtactatat ggtcctggtt ttattaatga agagctacac ggacttaaat ttacgctttc
     3481 tagtaaatca tttttccaag ttaatacact ccaggctgag gtgctttacg aaagagcact
     3541 aactctagcg ggcataacaa aaaatgatgt ggtgttagat gcttattctg gcacaggcac
     3601 aattgctatt ttagcagcaa aaaaagctaa acaagttatc ggtgttgaaa ttgttaaaga
     3661 cgctgttaaa gacgcagaag taaacgctaa acaaaataat attacaaatg ttaagttctt
     3721 taaagatgac gctggtgctt ttttaactag agaaataaag aactataact tcagcgttgt
     3781 catcgttgat ccaccacgta aaggattgag tgacgaattt gttaacactt tacttagagc
     3841 gctaccgcct aaaataatgt atgtatcatg tgaccccgcg actttagcaa gagacttaaa
     3901 aaagttaaca aagaagtata atgttgaaca tattgaatgt gttgacatgt ttccgcggac
     3961 cgctcac
//
LOCUS       DAOSLP010000061         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_20382_length_3918_cov_6.895677, whole genome shotgun sequence.
ACCESSION   DAOSLP010000061 DAOSLP010000000
VERSION     DAOSLP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20382_length_3918_cov_6.895677"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PK460_04820"
     CDS             <1..548
                     /locus_tag="PK460_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54428.1"
                     /translation="KIIIISGDLAAGKSVLAKKLSRHYQIPYFSKARFKEILGEKISF
                     EGIDENKNISRSSYALLRHIGWRVAQVKGSVILEANFHTELLEQIKRDVEFTGHKVEL
                     LYLTGDLDVLFERFLYREHYENRHPVHLTHPLRSLEEFENYILKLRNEPEVLPRKMLD
                     TTNCDQEMIFEKALKLIGSLD"
     gene            564..2630
                     /locus_tag="PK460_04825"
     CDS             564..2630
                     /locus_tag="PK460_04825"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE54429.1"
                     /translation="MSYTTDKIRNIIFLGHQGSGKTTLVEQIASNILKQPAGSVERKN
                     TISDFTPEEKARLTSCSMSVVSLDYNGYRLNLLDAPGNDDFVADVVGVLDFVKAAVLV
                     VDTGKIEVGTVKHFNLLKKYGVPTFIFVNKLDKELVNFDEVLEKITGTLGKKGVSFTY
                     PLNDGKVFNGFVDVTTLKAVKADGSKRVEVELDEATKAKVSEMNNALVEQVALTDDAL
                     LEKFFSGEELSAEEIANGLKMAVLSGEIAPVIVGSSTENIGVNTLVDMALTYLPSPAF
                     IKEMKGKDRGNNEVVRAISENGPFSAYVFKTVYDQYKGTTNYAFVVSGSLELGSDVYN
                     AELNSTMRASQMFSLLGAKQNNIQKAIAGDIVAFAKLDGITTGHTLCDKNDVVTFPRA
                     NYPTIVYRRAISAKNSRDEEKLVQALYRVAAEDPTLEITRVPETKQLLLGGLSDSHLN
                     FVLDKVKTTFGFEANTDAPQVIYRETVKREASAVGRYVKQSGGSGFYGVVEMRFGPSG
                     SDENVFTEEIFGGTVPKNYHPAVEKGFFESVQEGQLAGFPVIGMKATLFDGKYHSVDS
                     NEQAFRMAAILAYREAYMNCAPTLLEPIMKITISVTNEFTGNVMNDLNQRRARVLSMD
                     ESGYGTQEITALVPEAELLDYAIKLRVLSQGSGSFNSEFDSYQEVPTNLLQGVIAANS
                     RLEKDK"
     gene            complement(2693..3646)
                     /locus_tag="PK460_04830"
     CDS             complement(2693..3646)
                     /locus_tag="PK460_04830"
                     /inference="COORDINATES: protein motif:HMM:NF023156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HOE54430.1"
                     /translation="MEKRLRVALYLKMLVGFMISFIIAYLLKMPTSYTAGVIAVLNLW
                     YSRDNVVKTALIRVLSSLIGLATSALLFFLLGYNIFTLFFMVFIVLALLYVFKLEYGA
                     TPTLVLIGQQWAQQTSEAPLNAFVTLLIGVIPALLLNLITFRKSKILLINQQKLDTEI
                     ANIFTYFEKDEIYDFSVIDKILKDTRESLQIALDNYEVKNIMQNFHYINMRTEQINVL
                     KRIVNDLNMLEASPYKKEITNYLIKFKDRIGLEDYASDLLVKHDKLVAYYRALELPKT
                     RDEFEHRAILFAILEEIKIFLILKLDYHKIYPCQKQIETTK"
BASE COUNT         1298 a          652 c          779 g         1189 t
ORIGIN      
        1 ccaaaatcat aattatttca ggtgacctcg ccgctggtaa aagtgtgtta gcaaaaaagc
       61 tttcacgtca ttatcagatc ccttatttct caaaagctcg gtttaaagaa atactcggcg
      121 aaaaaatttc gtttgaagga atcgacgaaa ataaaaatat ttcgcgctcc tcatatgcgc
      181 tcttacgcca tattgggtgg cgggtagccc aagtaaaggg cagtgttatt ttagaagcta
      241 attttcacac tgaactctta gaacaaatta aacgtgacgt tgaatttact ggtcacaaag
      301 ttgagttgct ctatttaacg ggtgatttag atgtactatt tgaaagattt ttatatcgtg
      361 aacattacga aaatcgccat ccagtgcact taactcatcc actacgttca ttagaagaat
      421 ttgaaaatta tattttaaaa ttacgtaatg aacctgaagt attaccacgc aagatgcttg
      481 ataccactaa ttgtgatcaa gaaatgattt ttgaaaaagc actcaaactc attggtagtt
      541 tagattaata tgaaggagaa attatgagtt ataccacaga taaaattaga aacattatct
      601 tccttggcca ccagggctca ggaaaaacga cacttgtgga acaaatcgca agtaatatct
      661 taaaacagcc cgctggcagt gttgaacgga aaaataccat tagtgacttt acacctgaag
      721 aaaaagcgcg tttaacatca tgttcaatgt cggttgttag ccttgattat aatggctatc
      781 gcttaaactt acttgatgca ccaggcaatg atgactttgt tgctgatgtt gttggtgtgc
      841 ttgactttgt taaagcagca gtcttagtcg tcgatactgg taagattgaa gtcggaacag
      901 ttaaacactt taatttatta aagaagtatg gcgttcctac ttttattttt gttaataagt
      961 tagacaaaga attagttaac tttgatgaag ttcttgaaaa aattactggt acactcggta
     1021 aaaaaggcgt aagcttcact tatccgctta atgacggtaa agtatttaac ggttttgtcg
     1081 acgtcacaac gcttaaagct gttaaggcag atgggtcaaa acgcgttgaa gtcgaacttg
     1141 atgaagcgac taaagcgaaa gtcagtgaaa tgaataatgc gcttgttgaa caagtagcgt
     1201 taacggatga cgccctttta gaaaaattct ttagcggtga agagttaagc gctgaagaaa
     1261 tcgctaacgg acttaaaatg gccgtgttaa gcggtgaaat tgctccagtt attgttggta
     1321 gcagtaccga aaatatcggt gttaatacac ttgttgatat ggcacttact tacttaccaa
     1381 gcccagcttt cattaaagag atgaaaggca aggatcgcgg taataatgaa gttgtgcgtg
     1441 caattagtga aaatggacct tttagtgctt atgtctttaa aacagtatat gaccaatata
     1501 aaggcactac taactatgcc tttgtcgtaa gtggtagttt agaacttggt agtgatgtgt
     1561 ataatgctga acttaattcc acgatgcgtg cttcacaaat gtttagtctt ttaggcgcta
     1621 aacaaaataa cattcaaaaa gcgattgctg gtgatatcgt tgcttttgca aagttagacg
     1681 ggattaccac tggtcacaca ttatgtgata aaaatgatgt cgttacattc ccgcgcgcta
     1741 attatccgac gattgtttat cgccgcgcta tcagtgcgaa aaatagtcgt gacgaagaaa
     1801 agttagtgca agcactctac cgtgttgccg cagaagaccc aacccttgaa attacgcgtg
     1861 ttccagaaac aaaacaactc ttacttggtg gacttagtga tagtcatctt aactttgtgc
     1921 ttgataaagt aaaaactaca tttggctttg aagcaaatac cgatgcacca caagtaattt
     1981 accgtgaaac ggtaaaacgc gaagctagtg cagttggccg ttatgttaag caatcaggcg
     2041 gcagcggctt ctacggagtt gttgaaatgc gctttggccc aagtggtagt gatgaaaatg
     2101 tctttaccga agaaatcttc gggggaacag taccaaagaa ctatcaccct gctgtcgaaa
     2161 aaggattctt tgaaagtgtg caagaaggtc aacttgcggg cttccccgta atcgggatga
     2221 aagcaacatt atttgacggt aaataccaca gcgttgatag taacgaacaa gcgttccgca
     2281 tggccgctat tttagcctat cgcgaagctt atatgaattg tgccccgacc ttacttgaac
     2341 cgattatgaa aattaccatc agtgtcacaa atgaatttac gggtaacgtt atgaatgacc
     2401 ttaaccaacg tcgggcacgg gtattaagta tggatgaaag tggttatggt acccaagaaa
     2461 ttaccgcctt agtgcctgaa gcagaactat tagactacgc cattaaactt cgggtcttaa
     2521 gccaaggcag tggtagtttc aatagtgaat ttgatagcta tcaagaagta cccacaaact
     2581 tattacaagg tgttatcgct gccaattcac ggcttgaaaa agataaataa ttagatagtt
     2641 tacaaaaaga aaaccatcgg tgtgcgccga tggttttttg tatataatcg ctttattttg
     2701 tagtttctat ttgcttttga cacggataaa ttttgtgata atcaagttta agaattaaga
     2761 agattttaat ttcttctaat atcgcaaata atatagcgcg gtgttcaaat tcgtctctag
     2821 tttttggtag ttctagtgcg cggtaatagg ccactagttt atcgtgctta acgagtaaat
     2881 cacttgcata atcttcaagt ccaatgcggt ctttgaactt aattaaataa ttagtgattt
     2941 cttttttata tggtgaagct tctaacatat ttaaatcgtt tactattcgc tttaatacat
     3001 taatttgttc ggtgcgcata ttaatataat ggaagttttg catgatgttt tttacttcat
     3061 aattatctag cgctatttgc agactttcac gtgtatcttt gagtatttta tcaatcacgc
     3121 tgaagtcata aatttcatct ttttcaaagt aggtaaatat atttgcaatt tctgtatcta
     3181 gtttttgctg attaataagt aagatctttg atttacggaa agtaattaag tttaaaagta
     3241 aagcagggat aacaccgatt aaaagcgtga caaaggcatt taatggcgct tctgaagttt
     3301 gttgcgccca ttgttgccca attaagacaa gggttggggt tgcgccgtat tcaagtttaa
     3361 aaacatagag gagcgctaaa acgatgaata ccataaagaa gagcgtaaaa atgttatagc
     3421 ctaataaaaa gaagagcagt gcactagtgg caagcccaat taaacttgat aatacgcgaa
     3481 taagggcagt tttgacaaca ttatcacggc tataccataa gtttaaaaca gcaataacac
     3541 cagcagtgta gcttgtcggc atcttaagta aataggcaat gataaaacta atcataaagc
     3601 ccactagcat tttaagataa agcgcaactc gtaatctttt ttccatacat tagatataat
     3661 aacaaaaagt ttagtatagt aactctttaa agcattagta agataatagt ttgtaagcaa
     3721 tatataactt aaaaagtaat aaaattatgt tgatttgctc cataaagtgt tatttaaggc
     3781 aaggaagtgg agtaaaaaaa catttgccga cacttgatgt aaagaagaga caaagaatct
     3841 tttatataat agaaagcaag tattaatgaa cataatttga cgatatttac taaataacga
     3901 acataatatc aaaataat
//
LOCUS       DAOSLP010000062         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_23042_length_3554_cov_9.575593, whole genome shotgun sequence.
ACCESSION   DAOSLP010000062 DAOSLP010000000
VERSION     DAOSLP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23042_length_3554_cov_9.575593"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PK460_04835"
     CDS             complement(<1..247)
                     /locus_tag="PK460_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54431.1"
                     /translation="MHKGKRNFFAIVLTLLIALGLPKLSSNSTRDKVNASATETVYLK
                     VNDNWKIDNARFAAWVWNPGTWFDLTQVEGETHIYKAD"
     gene            601..915
                     /locus_tag="PK460_04840"
     CDS             601..915
                     /locus_tag="PK460_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54432.1"
                     /translation="MVKRDHPDWCLKGPTEITKEEYNELKAKLVNDPYWNKKTRQNTA
                     LFADKLIDEPNYTNRRGIKTNTATFKKPTTAEIKMFHQKKRKIRDKEIQEFYDEANDY
                     ES"
     gene            complement(970..1536)
                     /locus_tag="PK460_04845"
     CDS             complement(970..1536)
                     /locus_tag="PK460_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54433.1"
                     /translation="MKRIHYYIVGTIILFATVIFSNSVGATSGANKRMEKLEVKARDL
                     FVTLSERYTKIEELHDNLSALDETATALVNNIEERITNFNDTKLLSQTVWPALQDAKY
                     IDENFMTLILYLKDNKDTFGSTLPSSFYTEFDAITSVLLPKINAYNSVTADYNTHIDL
                     FPNRLYLKGRNPYIIFDLALYPENLPLF"
     gene            complement(1551..>3554)
                     /locus_tag="PK460_04850"
     CDS             complement(1551..>3554)
                     /locus_tag="PK460_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54434.1"
                     /translation="NQENNEVESTIENVADLPSESENPLNLSYFLDSIAIPKIVTLKD
                     LGDVTFEVTTNRPDLLFIGEMSKPIEDLDPGNELITGDVTVHTLTPVGTYSSEIPASV
                     DTVDKLKDHLKSVDVKELYPYATQPTQDFSITITATCFGEERTETYYFRLQEPPLDQE
                     IVDYAIKSEYMINKVFYKDTTSIIRTTRENMPSTPEKRMKVSYLTETLAIPRHVVLSD
                     FGNKELDFSINVLGNNADKFYMSEQTDTLDGNPVTVKTLTPVGGLAYTTPQNDLPAFA
                     EEIKGLSREDLYDCVTQTSIDVDIELSATLNGKTATKTYYFTLVAADIDVEIIDYLID
                     DSSLLNVVTYEDVNDDVKIQKIADQAKDPLNPYAIEHLVDTIVVPQSITLTNFGNREL
                     QIETTINSDDPGALNFYEASKSATISDNLVTAKTLTPLGSYDASAVDPNNLTALADSI
                     KALPTKDLYKHSQSVGYNVELNVTVTLVSGEGEPEVRSTTFYLKTIKADVDSKIGTAI
                     LDTYDIVNLIDHTDPYINVTNPPQPTDPLNPHQLLYFGETLVFPIEFEYTDFENKLIQ
                     FDVNTNGKDHLFFISQKTHHYEGKDVEGITLSPIGTYDPGEPLNTYQDLINHLKNDAN
                     IMAIAAARAVQVEENVTITVTISIDGREVAVNNYYFVLVVERS"
BASE COUNT         1124 a          615 c          648 g         1167 t
ORIGIN      
        1 tatcagcttt ataaatatgc gtctcgcctt caacttgcgt taaatcaaac caagtacctg
       61 ggttccacac ccatgctgca aagcgggcat tatcaatctt ccagttatca ttaactttta
      121 aataaactgt ttcagtagcg cttgcattaa ctttgtcacg cgttgagtta ctacttagtt
      181 taggtaatcc tagcgcgatt aaaagcgtta agacaatagc gaagaaatta cgttttcctt
      241 tatgcataat cgttcctacc ttctttgtac agattagttt ttgtgaagta gtaagtaaat
      301 taataagtaa tttacttttt aattaaaatt taattcatca cacttatctt taatattacc
      361 atattaaaca aatatcacac cttaaattta gcatgtgtat tcaaaaatac gcctattagt
      421 gttagaataa gagtgaattt aatattagtt aggagtaatt atggcctttt ttgccgagcg
      481 caaatattat gttgttacct gtaaatttgg tcacgttggt cgtgataagt atttaccact
      541 tgatacctat tagagcaaca agtaaaaaag aagcaagcgc taaagcaaaa aagactgggg
      601 gtggtaaagc gcgatcaccc tgattggtgt ttaaaaggac caacggaaat tactaaagaa
      661 gaatataatg aacttaaagc aaaattagta aatgatcctt attggaataa gaaaacacgg
      721 caaaacaccg ctctttttgc tgataagctc attgatgaac ctaattatac gaataggcgc
      781 ggcattaaaa caaatacagc tacctttaaa aaaccaacaa ccgctgaaat aaaaatgttt
      841 catcaaaaaa agcgcaagat tcgtgataaa gaaatccaag aattttatga tgaagcaaat
      901 gattacgaaa gttaaaaaat aaaatccacc tagtctatta gtaaggacac taaaaggtgg
      961 gtttttttgt tagaaaagtg gtaaattttc tggataaagc gctaaatcaa agataatata
     1021 cggatttcgt ccttttaagt agagtcggtt cgggaaaaga tcaatatgtg tgttgtaatc
     1081 agcagtcaca ctattataag cattaatctt tggtaataaa acacttgtta ttgcatcaaa
     1141 ctctgtgtag aaggaagaag gaagggtact accaaaagta tccttattat cctttaaata
     1201 aagtatgagt gtcataaagt tttcatcaat atacttagca tcttgcaatg caggccacac
     1261 agtttgagag agaagcttag tgtcattaaa attagtaatc cgctcctcaa tgttatttac
     1321 taaagcagta gcggtttcat ctaaagcgga taaattatca tgtaattcct ctatttttgt
     1381 gtagcgctca cttaaggtaa caaataagtc gcgcgctttt acttcaagtt tttccatgcg
     1441 tttatttgct ccgctcgtag cgccaacgga gttactaaaa ataactgtcg caaaaagaat
     1501 aattgttcca actatataat aatgaattcg cttcatttaa tcgtctcttt ctaacttcgc
     1561 tcaacaacta agacaaagta gtaattattg acagcaactt cgcggccatc aatcgatatt
     1621 gtaacagtta ttgttacatt ttcctcaact tgtactgctc tcgctgcggc aattgccatg
     1681 atattagcat catttttcaa gtgattaatt aagtcttggt aagtgttaag tggttcacct
     1741 ggatcataag tgccaattgg gctaagtgta atcccttcaa catctttacc ttcatagtga
     1801 tgggtttttt gtgagatgaa gaacaaatga tctttaccat ttgtattaac atcaaactga
     1861 atgagcttat tttcaaagtc agtatattca aattcaatcg ggaacacgag tgtttcacca
     1921 aaatagagta attgatgtgg attaagcgga tcagttggtt gtggtgggtt agtgacatta
     1981 atatacggat cagtgtggtc gataaggtta acaatatcgt aagtatctaa aatcgctgtc
     2041 ccaatcttgc tatcaacatc agccttaatt gtttttaagt agaatgttgt ggaacggact
     2101 tctggttcac cctcgccact aactaaagta acggtgacat taagttcaac attataacca
     2161 accgattgtg aatgtttgta taagtctttt gttggtaagg ctttaatact atcagcaagc
     2221 gctgttaagt tatttggatc gacagcacta gcgtcataac taccaagcgg tgttagtgtc
     2281 ttagcggtca ctagattatc actaattgtt gcgctcttac tagcttcata gaaattaagc
     2341 gcaccaggat catcactatt aattgttgtt tcgatttgta actctctgtt gccaaagttt
     2401 gtgagggtaa ttgattgcgg gacgacaatt gtatcaacga gatgttcaat cgcgtaagga
     2461 ttaagtgggt ctttagcctg atcagcaatt ttttgaattt ttacatcatc gtttacatct
     2521 tcataagtaa cgacattgag taaagaacta tcgtcaatta agtaatcgat aatttccaca
     2581 tcaatatctg cggccacaag tgtgaaataa tatgtttttg tcgcggtttt accgttaagt
     2641 gtagcactta gttcaatatc aacatcaata ctagtttggg taacgcaatc ataaagatct
     2701 tcacgcgaga gccctttaat ttcttcggca aatgctggta aatcattttg cggtgtcgtg
     2761 taagctaaac cgccgactgg tgttaatgtc ttaacagtga cagggttacc gtcaagcgta
     2821 tcagtctgtt cggacatata aaatttatca gcgttattac ctaatacatt aattgagaag
     2881 tctagctctt tgttaccaaa gtcacttaaa actacatggc gcggtatggc tagagtttcg
     2941 gttaagtacg ataccttcat ccgtttttct ggagtggaag gcatattttc acgtgtcgta
     3001 cgaataatac ttgtggtgtc tttgtaaaat actttattaa tcatgtattc gcttttaatt
     3061 gcataatcaa caatttcttg gtcaagcggt ggttcttgta gcctaaagta ataagtttct
     3121 gtccgttctt caccaaaaca agtcgcggta atggtgatgc taaaatcttg agttggttgc
     3181 gtagcgtaag gatataattc ttttacatcc acactcttta agtgatcttt taatttatcg
     3241 acagtatcga cacttgcggg aatttcacta ctgtaagtcc caacaggtgt taaagtgtgc
     3301 acagtaacat cccctgtaat taattcatta ccgggatcta aatcttcaat tggcttactc
     3361 atttcaccaa taaataataa atcaggacga ttcgtcgtga cttcaaaagt gacatcgccc
     3421 aagtctttaa gtgtaacaat ttttggaatg gcaattgaat caaggaaata agaaagatta
     3481 agtggatttt cgctttcact tggtaagtct gcgacatttt cgattgtact ttcaacttca
     3541 ttattttctt ggtt
//
LOCUS       DAOSLP010000063         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_23773_length_3464_cov_6.276621, whole genome shotgun sequence.
ACCESSION   DAOSLP010000063 DAOSLP010000000
VERSION     DAOSLP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23773_length_3464_cov_6.276621"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..745
                     /locus_tag="PK460_04855"
     CDS             197..745
                     /locus_tag="PK460_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOE54435.1"
                     /translation="MNRNQIEKVLMKDEKVLYTYKPMFIKTVIITVICYLFTLLFVLL
                     ALLIPASAESMEINVTGGIVALAVIGITVFFGVVLLLLALAYRNKFYAVTNKRFIIQS
                     GLLGIDFSSIPISGVKFISVNVSVLDKILGKGTGTVSFGTVSTPVTPGQGAKFNFINI
                     YDVYENYRIFKELSDKAQTEDK"
     gene            874..3090
                     /locus_tag="PK460_04860"
     CDS             874..3090
                     /locus_tag="PK460_04860"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOE54436.1"
                     /translation="MEKKSHNLPMPKVHSWQDVKDVYFLYITNAEDRANIEEAYNFAL
                     KKHEGQFRKSGHPYITHLIEVAFILAELTAGPSTIIAGLLHDVVEDTDVTVSEIDKMF
                     GKDVAMIVDSVTKIQRLKLSKKVDDSDFVYEDHRKIFLGMAKDIRVIIIKLADRLHNL
                     RTLYFLSHERQLRIAKETMAVYAPIAGRLGMGKIKAEMEDLSLKYIEPEAFNHVSELV
                     EQRSPNLQKALAEVSKRIADILFKNDIKFEMKFRVKSIYSIYKKMKEKDNDFERIYDL
                     LALRIITPTELNCYEILGLIHATYRPVTGRFKDYIAMPKSNMYQSLHTTIFTGDGNAL
                     EVQIRTPEMDRIADSGVAAHWAYKEGTNYNPEREQREIENKLHWLRDFVATGYETGNT
                     AKEYMDTITKEIFETSVYVFTPKGNVIELPAGATPLDFAYKIHTKVGDTAVGALVNDK
                     LVAMNTPLKTGDICEIRTSKTATGPTESWLNIATTNSARSHIRKVLAKKNQALTRDDN
                     IEKGRGSLNDAFKERGFAEHEVEPHVNKPEVLKHFNVNTYEDLLLLVYSRNITASAVL
                     DYLKLDRRDHMGKLLSKGRRVVEDAKNPVIVPGAGTVAVTLGNCCTPVPGDNIVGYIT
                     KGKGITVHRINCPNIARNLDRTIEVLWNPNSETKLYPLDISIKCNDRDNLLVDIMNAL
                     SQAKVSISKIKATYHPSTQTSVIDATILIPNVDKLNSVQNIILQVDSVYKIDRVIH"
     misc_binding    3190..3391
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1196 a          565 c          678 g         1025 t
ORIGIN      
        1 ttatcggttg gtataatgtt ccacaattag ccgctgatga tccagtgcaa gttgtgaaaa
       61 aagttgctcc ataattaata attaattatt aaatatacga ctcacggtgt ccccgtgagt
      121 cttttcttta ctatttcttt acaagtaaca taaatttaag catggtaaaa ttaaagtgag
      181 aaaggagaac ataattatga accgcaatca aattgaaaaa gtattaatga aagacgaaaa
      241 agtattatat acttataagc caatgttcat taaaacagta attattaccg ttatttgtta
      301 tttattcacg cttttattcg tcttactcgc tttactaatt ccagcatcag ctgaaagcat
      361 ggaaataaat gtaactgggg gaatagtggc acttgccgtt attggtatta cagtattctt
      421 tggtgtcgta ttactacttt tagctctagc gtaccgtaat aagttttatg ccgtcacgaa
      481 taaacgcttt attattcaaa gcgggctact tggcattgat ttttcaagta ttccaataag
      541 tggtgtcaaa tttattagtg tcaatgttag tgtgctcgat aaaatactcg gtaaaggtac
      601 tggtactgtg tcttttggta ctgttagcac accagtaaca ccaggccaag gcgctaaatt
      661 taatttcata aatatttatg atgtctatga aaattatcgc atctttaaag aattaagcga
      721 taaagcccaa accgaagata agtaattaag aatattactg acgaactcga ctctcaacag
      781 agagtctttt tttattaaaa gcagcttatt aagtaaagca taatcaaagc gattattaat
      841 ttgttataat taataagaaa ggaaacgcat agtatggaga aaaaaagtca taacctacca
      901 atgccaaaag tccattcgtg gcaagacgtc aaagatgtct attttctcta tattactaat
      961 gcagaagacc gcgctaatat tgaagaagca tataattttg cgcttaaaaa acatgaaggg
     1021 caatttcgta aaagcggtca tccttatatt acgcatttaa ttgaggttgc ctttattctt
     1081 gctgaactaa cagcgggtcc aagtacgatt attgctggtt tactgcatga tgtcgttgaa
     1141 gacactgatg taacagttag tgagattgat aaaatgtttg gcaaagatgt ggcgatgatt
     1201 gtcgatagcg tcacgaaaat tcaacgccta aaacttagta aaaaagttga tgatagtgat
     1261 tttgtgtatg aagatcaccg taaaatattc ttagggatgg caaaagacat ccgtgttatc
     1321 attattaaac ttgcggacag attacataat ctgcgaacat tatacttttt aagccatgaa
     1381 cgacaactgc gtattgctaa agaaacgatg gcggtttacg ctccgattgc tggacgccta
     1441 gggatgggga aaattaaagc agaaatggaa gacttaagtc taaaatacat tgaaccagaa
     1501 gcctttaatc atgtaagtga actcgttgag cagcgctccc caaacttaca gaaggcgctt
     1561 gcggaagtta gtaaacgaat tgcggatatc ttatttaaaa atgacattaa atttgaaatg
     1621 aagtttcgtg tgaagtcgat ttatagtatt tataagaaga tgaaagagaa agataatgac
     1681 tttgaacgga tttatgacct tttagcgctt agaatcatta ctccaacgga acttaattgt
     1741 tatgaaattt taggactaat ccacgcgact tatcgcccag ttacgggacg ctttaaagat
     1801 tatatcgcga tgccaaaaag caatatgtat caaagtttac acacaactat ctttacaggg
     1861 gacggtaatg ctttagaagt gcaaataaga acgccagaga tggaccgtat cgctgactca
     1921 ggggttgcag cgcactgggc atataaagaa ggtacaaatt ataatccaga acgcgaacaa
     1981 cgtgagattg aaaataaatt acattggctc cgtgattttg tggcaacggg ttatgaaact
     2041 ggtaataccg cgaaagaata catggacacg attacaaaag aaatctttga aactagtgtg
     2101 tatgtcttta cgccaaaagg taatgttatt gaactcccag cgggagcaac accacttgac
     2161 tttgcctata aaattcacac taaagtcgga gatacagcgg ttggggcact tgttaatgat
     2221 aagcttgtag caatgaatac gccacttaaa accggtgata tttgtgaaat tagaacgagt
     2281 aaaacggcta caggaccaac agagtcatgg cttaatattg ccaccacgaa tagtgcacgc
     2341 agtcatattc gtaaagtgct agcgaagaaa aatcaagctt taacgcgcga tgataatatc
     2401 gaaaaagggc gtggctcact aaatgacgcc tttaaagaac gcggctttgc cgaacatgaa
     2461 gttgaaccac atgtgaataa accagaagtc ttaaaacatt ttaatgttaa tacttatgaa
     2521 gacttactat tacttgttta ttcgcggaat attaccgcta gtgcagtatt agattactta
     2581 aaacttgatc gccgtgacca tatgggcaaa cttttaagta aaggacgaag agtagtagaa
     2641 gacgctaaaa atcccgtaat cgtcccaggg gcgggcactg ttgcggtcac acttggtaat
     2701 tgttgtacac cagtgccagg tgataatatc gttggttata tcacgaaagg gaaagggatt
     2761 accgttcacc gcattaattg tccaaatatc gcgcgtaacc ttgaccgcac gattgaagta
     2821 ctatggaatc caaatagcga aactaaatta tatccgttag atattagtat caagtgtaat
     2881 gaccgtgata acttattagt cgatattatg aatgctttaa gtcaagcaaa ggttagtatc
     2941 tcaaaaatta aggctactta tcacccatcg acccaaacaa gtgtgattga tgcaacgatt
     3001 ttaattccga atgttgataa attaaatagt gtgcaaaata ttatcttgca agttgactca
     3061 gtttataaaa ttgaccgcgt aattcattaa aataacgcta aaagtatttt attagtagtt
     3121 taatagtaga taattagtga ataatagtcg ccgcttacat tgactattaa agttaattat
     3181 tttataatta aaaagtcatt gatatggatt ttaacaaata ttccggaata gagactagtt
     3241 ggttggtgca aaactagcga agtattgtta aaggacacca tggagacaag tcttgtaata
     3301 aataagcagg accgttactc acgttacgag ttagagatgc gtttaattaa caaacgaaat
     3361 aaggtggtac cgcggcaatc ccgtccttat actacctttt tttatttaga ggagattaac
     3421 tattcttaat caagttaaat agtaaatata tatcaaaaag attc
//
LOCUS       DAOSLP010000064         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_24710_length_3360_cov_7.360968, whole genome shotgun sequence.
ACCESSION   DAOSLP010000064 DAOSLP010000000
VERSION     DAOSLP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24710_length_3360_cov_7.360968"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PK460_04865"
     CDS             <1..1195
                     /locus_tag="PK460_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOE54437.1"
                     /translation="TSSVGTNPGGLGETVLSHITVDTTDAKTTYFLGDGFTTNSLIVT
                     AHFSDETSLPVLDYTSSIANGTALNTLGNQSVTISYTHEGVTKSTSYTILVKEFVVSD
                     YLFISEVYGGGGNAGAEYKTDFIELYNYADFPLSLEGYSVQYASAAGTSWQVTNLSGT
                     IAPKSFYLIEEASGSGGTRNLPLAETVGSIPMGAKSLKLALVNSTTALSGANPTSDTS
                     IIDFVGVGTANAYLGLTAAPEISNTMSAQRKFIIEGIAPPVEKSNGTDFIAALPTPTN
                     AALTIAVDIRAEGDTPNQCLTLYSPYKSRVLSLSPSQLNYFKTVVAGEEERLTDGRAR
                     YEAWALHLGDALPYEYNENFGLGNTNKQTFTSDIVMLVLVIALFGLSIGFYFIKSKQN
                     TAKTH"
     gene            complement(1272..2258)
                     /locus_tag="PK460_04870"
     CDS             complement(1272..2258)
                     /locus_tag="PK460_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54438.1"
                     /translation="MKNKLFSTVMLLVLLLTGCKEGSSSITKSEEPSAPSTETSVVIE
                     DSSEAASEYAGLIGMDTYNDWPSAAIDEYLLSHNVEEKVPALPNVTRSYVELTLDDPN
                     FEHYLTVFIPGTNQAEDYTAILQAVNYQVVYYEEDDYYIGLNSETTIQIDYFYVTGDA
                     DYPDGMFIFIEALATNGGGTIVTPEGIPSVIDFATEDQITKRDALQSIWTTESGVTMT
                     IDKGASTVNVGNLTTQNEVGYFTNPLRIYAGQVVTFTVPAEVQINAVLLETAGAWEKG
                     DSVLAVTNSAPETVDFIAEGDNVLFTLFSPSNTLTFSLAAQARFNKITVFVI"
     gene            complement(2425..3309)
                     /locus_tag="PK460_04875"
     CDS             complement(2425..3309)
                     /locus_tag="PK460_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54439.1"
                     /translation="MKKRKILLLPMLLALLLTGCDKGGESQTSQGGGGTTSQTGGGTT
                     SQGGETSSSTDFFVDNGYTKTKGWPTALIGEFLSRNGITDTIPELSNVNTLYYATSED
                     PANYDYFSLAIPGSDRSSEYKNKLTGAGYTIKPIDAAFAGVSRNETIGVTLGYLKNYE
                     GAPDALYILFVPNTPDSFGLHLFEEVRGWPTEVITSYLRRVGVAETLPALPNVTKSYY
                     ASMMSDGEEMLGVFVPGGDRSEEYGEVLTANGFMSTMGMYFNPEMTIIVFDKYFAKSD
                     YSIIEGTLFIIMASANAE"
BASE COUNT         1051 a          621 c          599 g         1089 t
ORIGIN      
        1 aacatctagc gttggcacta atcctggtgg tcttggtgaa acggtacttt cacatataac
       61 agttgatact acagacgcta aaacaactta ctttttagga gatggtttta ctacaaatag
      121 cctcatagta accgcacatt ttagtgatga aacatcacta ccagtgcttg attacacttc
      181 aagcattgct aatggtacag ctttaaatac acttggaaat caaagtgtta ctattagtta
      241 tacacatgaa ggagttacta aaagcacctc atacactatt cttgttaaag agtttgtcgt
      301 aagtgattat ttatttataa gtgaagtata tggtggtggt ggtaacgctg gggcggaata
      361 taaaactgac tttattgaat tatataatta tgctgatttt ccgctatcac ttgaagggta
      421 ctctgtgcag tatgcttctg cagctggaac aagttggcaa gtgactaatt taagtggtac
      481 gattgcacca aaatcatttt atttaattga agaagctagt ggttctggtg gaacgagaaa
      541 cttaccatta gcagaaacag tcggttctat tcccatgggt gcaaaatctt tgaaactggc
      601 gttagttaat tcaacaactg cgctttcagg tgcgaatcca acaagtgata catctattat
      661 tgattttgtt ggtgtcggaa cggctaatgc atatcttgga ttaacagcag cacctgaaat
      721 tagtaatacg atgtctgccc aaagaaagtt tattattgaa ggtattgctc ctccagttga
      781 aaaaagtaat ggaacagatt ttattgctgc tttaccaaca ccaacaaatg ctgctcttac
      841 tattgctgtt gatataaggg cagaaggcga tactcctaat caatgtttaa ctttatatag
      901 tccttataaa tcaagagtgc tttcgcttag tccgtcgcag cttaattact ttaaaacagt
      961 tgtagcgggt gaagaagaaa gattaacaga tggtcgcgcc cgatacgaag cgtgggcact
     1021 tcatttaggt gatgctttac cttatgaata caatgaaaac tttggacttg gaaacacgaa
     1081 caagcaaaca tttactagcg atattgttat gcttgtcctt gtcattgctc tctttggttt
     1141 atcaatcgga ttctatttta ttaaatcaaa acaaaacaca gcaaaaacac attaaaattt
     1201 gcaaaaacag cttattttat ttaaaaaaga gggccaaaac ctcttttgaa atatcttatg
     1261 attctatttg attaaataac aaaaacagtt attttattaa atctagcttg cgctgctaat
     1321 gagaatgtaa gtgtgttact tggagaaaat aaagtgaaaa gtacattatc accttcggca
     1381 ataaagtcga ctgtttctgg agcggaatta gtaaccgcta aaacagagtc acccttctcc
     1441 caagcgcccg cagtttctaa taacacagca ttaatttgta cttcagcagg aacagtaaaa
     1501 gtaactactt gtccagcgta aatccgcaac ggatttgtaa agtaacctac ctcattttgg
     1561 gttgttaaat ttcctacatt tactgtcgat gcacctttat caatcgtcat tgtgacacca
     1621 ctttctgttg tccaaattga ttgcaaggca tcgcgttttg ttatttggtc ttcagtagca
     1681 aaatcaatga ctgatggaat accttctggg gtaactattg ttccgccgcc gttagttgcg
     1741 agtgcttcaa taaaaataaa cataccatca ggataatcag cgtcaccagt tacatagaaa
     1801 taatcaattt gaatcgtggt ttcactatta agtccaatat aatagtcatc ttcttcataa
     1861 tatacaactt gataatttac tgcttgtaaa atagcggtat aatcttcagc ttgatttgtg
     1921 ccgggtataa acactgttaa atagtgctca aaatttggat catcaagcgt taattcaaca
     1981 taacttcttg ttacatttgg tagcgctggt accttttctt ccacattatg actaagtaaa
     2041 tattcatcaa tagcggctga tggccaatcg ttatatgtat ccatgccaat taatccagca
     2101 tattcacttg ctgcttcgct tgaatcttcg atgactacac tagtttcggt gctaggagcg
     2161 ctaggttctt cacttttagt tatcgaagat gaaccttctt tacaaccagt taatagtaaa
     2221 actaaaagca taactgtact aaataattta tttttcatag tttcttctcc tttaataata
     2281 ggaattatac taatattgct ttttatttca aaataaaagt actttaaatc aaaagaaaaa
     2341 tggccgctag tggatagcga ccattgttga aagttttaat aataaactat aagcattaat
     2401 tgtaaacgaa tagataatgt atgtttattc agcgttagcg ctagccataa ttataaataa
     2461 agtgccttcg attattgagt aatcgctttt tgcaaaatat ttgtcgaaaa caataatagt
     2521 catttctgga ttaaaataca tgcccatagt agacataaag ccgtttgcag ttagaacttc
     2581 gccatattct tcactacgat caccacctgg tacaaaaaca cctaacattt cttccccatc
     2641 actcatcatc gaggcataat agctttttgt aacatttggt agggctggaa gcgtttctgc
     2701 tactccaact cttcttaagt atgaagtaat aacttctgta ggccagccac gtacttcttc
     2761 aaacaagtga agaccaaagg agtccggtgt atttggtacg aaaagtatgt ataaggcatc
     2821 aggtgcccct tcataattct ttaagtatcc aagtgtaact ccaattgttt catttctgct
     2881 aacaccagca aaggctgcat caattggctt aattgtgtaa ccagcaccag ttagtttgtt
     2941 tttatattct gatgaacgat cgctacctgg tattgctagc gaaaagtagt catagttagc
     3001 cggatcttca ctagttgcat aatacaaagt attaacattt gagagttctg gaatggtgtc
     3061 agtaattccg ttacgtgaaa ggaattcgcc aattaaagca gtaggccatc ctttggtttt
     3121 ggtataacca ttatcgacaa agaaatcagt cgatgagcta gtttcaccac cttggctagt
     3181 agtaccgcca ccagtttgac ttgtggtgcc gccaccgcct tgagatgttt gtgattctcc
     3241 gcctttatca catcctgtta aaagtaatgc taaaagcatt ggcagtaata aaatttttct
     3301 ttttttcata ataaccttgt attatctaat catgccccgg tacttcgatt aggtaataca
//
LOCUS       DAOSLP010000065         3190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_26415_length_3190_cov_10.734609, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000065 DAOSLP010000000
VERSION     DAOSLP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26415_length_3190_cov_10.734609"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..268)
                     /locus_tag="PK460_04880"
     CDS             complement(8..268)
                     /locus_tag="PK460_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOE54440.1"
                     /translation="MKGIILYFSKYGATKKYVDWLSKSTNFDVVPVKKAKKSHIKDYD
                     VIILGSGIYASGIKGVKFLRKHAKLLSALKEKVKFTSFLSSL"
     gene            complement(270..1595)
                     /locus_tag="PK460_04885"
     CDS             complement(270..1595)
                     /locus_tag="PK460_04885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE54441.1"
                     /translation="MNNYDVIIVGAGTAGTYFAYLLGKRGLKVLVIDKDKEKDLAKRL
                     DIFHFTTESFARFNLPESKAGDEEFVSKFSYIYSRSALDNYEKKNFLEVSVMHLPLFI
                     KRLRNKAIKENVEFKFATEFKDLIYDAKGRISGIETKDEKSYNARLVVDASGIPSVVR
                     RVVKDPYMETFPIGPRDKFYVFLKYVKLKDPNIKIDMSLSWPYYKSWIAPQQNKDGAI
                     VGVGANISVDYARKCMKKFEDSIKLPAYDLQYEEWGATPYRRPPYSFVSDGFLVLGDA
                     ACLTQPFNGEGITAAWVQSTPAADVVANALKDDKYPTKEALWPINVMYQTGEGALFAG
                     QRALLVGAVNMSKEDNDYLYANGIIFKSDDEEVKGSVAGNLIKGVLKGKFSFAALRSL
                     MGASSTGNKLTKHYKNYPTSPEKYFAWKKKADRLWKKAGSMADNVKDAD"
     gene            complement(1729..2307)
                     /gene="pduL"
                     /locus_tag="PK460_04890"
     CDS             complement(1729..2307)
                     /gene="pduL"
                     /locus_tag="PK460_04890"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOE54442.1"
                     /translation="MKKILIETSARHIHLSEKDLKILCGEDATLEVRGPLSQPGQFST
                     TTRLTIVGAKRNIERVTVLGPTRKDTQVEVSLTDAFTLGLDVPVRESGDIKGTPGVKL
                     VGPAGEVTLEQGLIVAKRHIHMTPADAKEFNVKNGEVVGVRVKTCDRSLVFEDVVVRV
                     REDFALAMHIDTDEANAAGICGEVYGEIVKLA"
     gene            2450..2983
                     /locus_tag="PK460_04895"
     CDS             2450..2983
                     /locus_tag="PK460_04895"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOE54443.1"
                     /translation="MLKKFLSNKQNQAEILRFIIVGVICTLVDFGVSSLIQFVIYPVA
                     EALKIGPFTITPNIFLAALFGFIFGVITNYILSVIVVFKNVENKKTSRSAKGFIIFVL
                     LSTGGFLINYAIKELGNLIIPMDTNYIWFVFIFGVATFVVLIYNYVTRKLILFKPKKE
                     EMIKSDENTTTSSQEKE"
     gene            3047..3130
                     /locus_tag="PK460_04900"
     tRNA            3047..3130
                     /locus_tag="PK460_04900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3081..3083,aa:Leu,seq:gag)
BASE COUNT          948 a          623 c          519 g         1100 t
ORIGIN      
        1 ataaacatta taatgaagac aagaaggatg taaatttaac cttttccttt aacgcgctta
       61 ataatttagc gtgtttacgc agaaatttaa cacctttaat tcctgaagca tatataccgc
      121 taccaagaat aattacatca taatctttga tatggctctt tttagctttt ttaactggga
      181 caacatcaaa gttagtactc ttgcttagcc aatcgacata ttttttggtc gctccgtatt
      241 tagaaaagta gagaattatt cctttcaact taatcagcat cctttacatt atccgccatt
      301 gaacctgctt ttttccacaa gcgatcagct tttttcttcc acgcgaaata tttttctggt
      361 gaagtaggat agtttttata atgttttgtt aatttgttgc ccgtgctact agcgcccatt
      421 aaactacgaa gtgccgcaaa cgagaacttt ccttttaaga cacccttaat taagttaccc
      481 gctacacttc cttttacttc ttcgtcatca ctcttaaaga taatgccgtt tgcgtataag
      541 taatcattat cttctttact catattaacg gccccgacaa gaagcgcgcg ttgaccagca
      601 aagagtgcac cttctcccgt ttgatacata acattaattg gccataatgc ttcttttgtt
      661 ggatatttat catcttttaa ggcgttggcg actacatctg cggctggcgt cgattgcacc
      721 caagcagcgg taatgccttc accgttaaaa ggttgcgtta agcatgctgc atctcctaag
      781 acaagaaaac cgtcacttac aaaactatag ggcggacgcc gatatggggt tgcgccccac
      841 tcttcatatt gcaaatcata tgcgggtagt ttaatactat cttcaaactt cttcatacat
      901 ttccgcgcat aatcaacgga tatattcgcg ccaaccccga caattgctcc gtctttattt
      961 tgttgtgggg cgatccagct tttgtaataa ggccaactta aagacatatc aatcttaatg
     1021 ttcggatctt ttagttttac atattttaag aacacataga acttgtcgcg tggaccaatt
     1081 ggaaatgttt ccatgtatgg atcttttact actcttctta cgacactagg gattcctgaa
     1141 gcatcaacaa caagccgagc gttataactt ttttcatcct ttgtttctat cccgcttatt
     1201 cgtcctttag cgtcataaat taaatcttta aactctgtag caaacttaaa ttcaacattc
     1261 tctttgattg ccttattgcg tagtctttta ataaataaag gcaaatgcat gacgcttact
     1321 tctaagaaat ttttcttttc ataattatca agcgctgaac gcgaataaat ataagaaaac
     1381 tttgagacaa actcttcatc acctgctttt gactccggta aattaaagcg ggcaaaagat
     1441 tctgtagtaa agtggaaaat gtctaagcgt ttcgctaaat ctttttcttt atctttgtcg
     1501 attacaagta cttttagccc gcgtttccct agtaaataag caaaataagt gccagcggta
     1561 cctgccccga caataattac atcataatta ttcatatgta tccctccatt tttattgtga
     1621 catatttatt actaaaagta attagtgttt acgataattt taaaatttat aaacaaaaaa
     1681 acatctcgtg aagtagatgt ttaatgtttt aataataatt agattaatct aggcaagttt
     1741 tacaatttcg ccataaactt cgccacagat acccgctgcg ttagcttcgt cagtgtcaat
     1801 gtgcatagca agcgcaaagt cttcacgaac ccgcacgaca acgtcttcaa acacaagact
     1861 acgatcacat gttttaacac gaacgccgac gacttcacca tttttaacat tgaactcttt
     1921 tgcgtcagct ggtgtcatat ggatatgacg ttttgccacg attaaacctt gttctaaagt
     1981 aacttcacct gctgggccaa caagcttaac acctggggtg cctttaatgt caccactttc
     2041 acgcactggg acatctagtc ctaaagtaaa agcatcagtt agtgaaactt caacttgcgt
     2101 atctttacgc gttggtccta acacagtaac gcgttcaata ttacgtttcg caccaacgat
     2161 tgttaaacgt gttgtcgtgg aaaattgccc gggttgacta agcggtcctc taacttctaa
     2221 agtagcgtct tcaccacata atatttttaa gtctttttca cttaaatgaa tgtgacgtgc
     2281 agatgtttca ataagtattt ttttcatgta actttctcct ttatatagca cataaattat
     2341 aacaataatc atcttatttt gctattgact cactattaat agtgaaattg aaggcggatt
     2401 tagttaaaaa tgataatata ttatttaccg agaaaggaaa ttcttagtta tgttaaagaa
     2461 gtttttatca aataaacaaa accaagctga aatattacgc ttcattattg ttggtgtaat
     2521 ttgtacttta gtggattttg gtgttagttc actgattcaa tttgttattt accccgtcgc
     2581 tgaagcactg aagattggac cctttacaat tacaccaaat atatttctcg cagcgctttt
     2641 tggttttatc tttggggtaa tcactaacta cattctttcc gttattgttg tttttaaaaa
     2701 tgtcgaaaat aagaaaacat cccgttcagc gaaaggtttt attatttttg tgctgttatc
     2761 cactggtggt tttttaataa attatgccat taaagaatta ggtaacttaa ttattccgat
     2821 ggacactaat tatatttggt ttgtattcat ctttggtgtt gcgacattcg ttgtcttaat
     2881 ttataactat gtgacgcgta aacttatttt atttaaacct aaaaaagaag aaatgataaa
     2941 aagtgatgaa aacaccacta cttctagtca agaaaaagag taaataacaa tcttttgtat
     3001 tattgtgatt gaaacattta taaaagttgt tataatttaa ttcgacgcag ccatggcgga
     3061 actggtagac gcgtacgttt gaggggcgta tggtaatccc gtgtgagttc aagtctcact
     3121 ggctgcacca gttatcaaaa attagtccta cttcgtaggg ctttttttat atttcatcat
     3181 agtcattttt
//
LOCUS       DAOSLP010000066         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_32454_length_2720_cov_10.363977, whole genome shotgun
            sequence.
ACCESSION   DAOSLP010000066 DAOSLP010000000
VERSION     DAOSLP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32454_length_2720_cov_10.363977"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="PK460_04905"
     CDS             complement(<1..1304)
                     /locus_tag="PK460_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE54444.1"
                     /translation="METSDKKVKSAHSRTAGTWFKDFLSTIGGFFVRLGKGIVNFFID
                     TGIAIKNTFVNLWKMIKNFFVSVYTHFVKGGWRTKLSHFIMGSGQMSMGRPIKGIIYL
                     LIQVAYFIFMFVPGGGLAWLIKFRTLGEVAVEYMAECTWPPGAPIEDCDPDFITYEKP
                     IFKDNSLLIMLFSVATILITVFFIVMYFVQVRGTFKAEKAYNEAKAKYELAKSEGSEE
                     AFKNPLPKFTDELKSLLNERFHFTTLSLPAITLTMFTILPLVFMILLAFTNYNRDTAP
                     PNKLFTWVGLDTFKNLFSTKGASATNATFGSAMVRILKWTFTWAFFATFTNYIFGLIL
                     AMIINRKSIKLKKLWRTIFVISIAVPQFVTLLLMNQLLKEYGPLNETLLQWGWIRQRI
                     KFLDDPVLAKITVIVVNIWVGVPYTMLLTSGILMNIPTELYES"
     gene            complement(1406..>2720)
                     /locus_tag="PK460_04910"
     CDS             complement(1406..>2720)
                     /locus_tag="PK460_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE54445.1"
                     /translation="EFMFKQKRIALLVAGLLLLAGCNGGSSADVSGDVSQGTTSQTTT
                     TSGGGSKERVRLSVWGSSAEEPLYHKHATEFQEKHPEINFSISYADVGEADAATNVLQ
                     DVSTAADVFFFADDQLPTMQAREVIAKLPKGYANKVKERDVASAAENASSAVDDELYA
                     FPVTNDNGYMLIYNKALLTETDVETLEGIMAKTTAEHQFVVDMGNGYYATSFIQYLCD
                     ITYNPITRLHTTNFNSAEAVDALQGVSNLLKPRVDNGFKSVDFNGAALDDLSDANGNK
                     VIAAVTGVWNTELLKEKLGENLGATKLPTFKSANGTTVQWGSFAGSKLVGVKSSSNNL
                     GWALAFADYITDEAAQRYRFETNGWGPSNKNLLADEELLADHVTLKALGAQAPYAISQ
                     AKSVGSKFWDNAAIVGNFLWKGPASEESPQTLQATLDAFVDALTK"
BASE COUNT          805 a          532 c          536 g          847 t
ORIGIN      
        1 gattcatata attcagtcgg aatattcatt aagatacccg acgttaatag cattgtgtaa
       61 ggaaccccaa cccaaatatt tacgacaatg accgtaattt tagctaggac tggatcatcg
      121 aggaatttaa tccgttgcct aatccatccc cactgtaata gtgtttcatt aagcggtccg
      181 tattctttaa gtaattggtt cattaagagc aaggtaacaa attggggaac ggcaatggaa
      241 ataacaaaga tggttcgcca cagtttcttt aatttaatgc tcttgcgatt aataatcatt
      301 gcgagaatta atccgaaaat atagttagta aaagtcgcaa agaatgccca ggtaaatgtc
      361 catttcaaga ttcttaccat cgcactacca aatgtcgcat tagtagcgga agcgcctttt
      421 gtactaaata aatttttaaa tgtgtctaac ccaacccaag taaataattt attcggcggg
      481 gcagtgtcgc ggttatagtt agtaaacgcg agcaaaatca taaagacaag cggtaatatc
      541 gtaaacatcg ttaatgttat tgccggaagt gagagcgtcg tgaagtggaa cctttcattt
      601 aataatgact taagttcatc agtaaatttt ggaagtgggt tcttaaatgc ttcttcacta
      661 ccttcacttt tcgcaagttc atattttgct tttgcttcgt tataagcttt ttctgcttta
      721 aaagtgccgc gaacttgaac aaagtacata acgatgaaaa agacggtaat taaaattgtt
      781 gcgaccgaga ataacataat taagagcgag ttatctttaa agataggttt ctcatatgta
      841 ataaaatctg ggtcacaatc ttcaatagga gcacctggtg gccaagtaca ttccgccata
      901 tattcaaccg caacttctcc gagtgttcta aatttaatta accatgctag tcctcctcct
      961 gggacgaaca tgaaaatgaa atacgctact tggattaaaa gataaataat gccttttatt
     1021 ggacggccca ttgacatttg tccacttccc attatgaagt gcgagagttt agtgcgccat
     1081 cctcctttga caaagtgtgt gtaaactgat acaaaaaagt ttttaatcat tttccataaa
     1141 ttaacgaaag tgtttttaat ggcaattcct gtatcaataa aaaaattgac gattcctttg
     1201 cctaaacgca caaagaaacc accaattgtg gacaagaaat ctttaaacca agtgccagcc
     1261 gtacgtgagt gggccgattt gactttttta tcacttgttt ccatatttta tggcctcatt
     1321 tctagaaaga taggagagcc agcgatgaac tggctcccaa aattaattaa aaattaatcg
     1381 attatatgtg tttaataatt ttatattatt ttgttaaggc atccacaaag gcatcaagtg
     1441 ttgcttgaag tgtttgtggt gattcttcac ttgctggacc cttccataag aagttaccaa
     1501 cgatagcagc attgtcccag aatttggagc caactgattt tgcttgtgaa atagcatatg
     1561 gcgcttgggc accaagtgct ttaagggtaa catggtcggc taagagttct tcgtccgcaa
     1621 gtaagttttt gtttgatgga ccccatccgt ttgtttcaaa acgatagcgt tgggcggctt
     1681 cgtcagtaat gtagtcggca aaggcaagtg cccatccgag gttattacta gaagatttaa
     1741 caccaactaa tttggaacca gcaaaggaac cccattgtac ggttgttccg tttgcactct
     1801 taaatgttgg gagcttagtg gcaccaaggt tttcacctaa tttttctttt aagagttcgg
     1861 tattccacac accagtaaca gcagcgataa ctttgttacc attggcatca cttaagtcgt
     1921 caagtgcagc accgttaaag tcaactgatt taaatccgtt gtctactctt ggttttaaga
     1981 gattgctaac accttgtaag gcgtcaactg cttcagcact gttgaagtta gtggtatgaa
     2041 gtcttgtaat tggattataa gtgatatcac agagatattg aatgaaggaa gttgcataat
     2101 acccattacc catatcaacg acaaattgat gttcggcagt agttttcgcc ataattcctt
     2161 ctaaagtttc gacgtcggtt tctgttaaga gtgccttatt gtaaataagc atatagccgt
     2221 tgtcatttgt aacagggaat gcataaagtt catcgtccac tgctgaactt gcgttttcgg
     2281 cagcactagc aacgtcacgc tctttaactt tattcgcata accttttggt aattttgcga
     2341 taacttcacg agcttgcatt gttggaagtt ggtcatctgc gaagaaaaat acgtcagcag
     2401 cagtacttac gtcttgaagg acgtttgttg cggcatcagc ttctccaacg tcagcgtaac
     2461 taattgagaa gttaatttct ggatgcttct cttggaattc agtagcgtgt ttgtgataga
     2521 gtggttcttc agcacttgag ccccacactg atagacggac acgctccttt gatccgcctc
     2581 cactcgttgt tgttgtttgg cttgttgttc cttgactaac atcaccactg acatcagcgc
     2641 tactgccacc attacagccg gcaagtaaaa gcaaacctgc aacaagtaaa gcgatacgtt
     2701 tttgtttaaa cataaattct
//
LOCUS       DAOSLP010000067         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_35378_length_2544_cov_4.812776, whole genome shotgun sequence.
ACCESSION   DAOSLP010000067 DAOSLP010000000
VERSION     DAOSLP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35378_length_2544_cov_4.812776"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PK460_04915"
     CDS             complement(<1..629)
                     /locus_tag="PK460_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54446.1"
                     /translation="MKNRKFLLLIVLMMLLSSCTTISEVLSSTTRSSDSPTSDSVSDT
                     ESSEEKVTSEEVPSSEEELSSEVLSSVSDPSSEETPSDITPADLTNFKTTYPLDPEIT
                     YTDEDYIVRPFPNGFTYPLDAHVAPTNAYLEFWHPETELNMRIVASKEVFNLIEQNGY
                     HNPRFADCYFPVTLEVLMNGKRFIYYEVGMRMKGNTSRTEFLDPDTKEFT"
     gene            complement(751..2307)
                     /locus_tag="PK460_04920"
     CDS             complement(751..2307)
                     /locus_tag="PK460_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54447.1"
                     /translation="MKKNHFKNRFLMFLTTLAMAFSVGVSLNAKAPVKAAALPAKIYL
                     KVSEGWKSDGARFETYFFEGTDPDLFVTMQRTLYADDLYEVVKPAGNHAKFIMVRMDP
                     AKPENDWDSKWNQSGDLVYDATKELVRVTGWTVGEPEAFVASEHINPAIAGDTVLYFR
                     PDASYFAEGYDHLAAYFFVGGENQFVNLVEHQTGVYKVTAPIPAAPGGWEKVIFVSLK
                     SATNAWEVDGLTNVLKQTPDLIYDGTKDMYDTTLGAWKTLGSLVSQTPTTAGISSEKV
                     RIWVNRGGHYATEGYQYLLKAGDVHYTATGYEKALVTDDVYFAYFDVPLSALTGKNVD
                     LVIVDTNLYFQVAVAGEPYAAQDNSKLWVVKQNMDETYSVTKGAVEGRIYNTFFAKVL
                     EGYLTCSTSADNGYEAFNLVDTNFLPRAGEHWDMQGDLGGEHGVKIADYTSEEDYATG
                     VREATPATDAYAKYVMMKSLYDAAHPSPEQFRINKVANNNIIYVLVIGMLFAIAAFGF
                     VRFRKKANVA"
BASE COUNT          771 a          521 c          445 g          807 t
ORIGIN      
        1 gtaaattctt ttgtgtcagg atctaagaat tccgtgcgtg acgtattacc tttcatccgc
       61 atcccaactt cgtaatagat aaaacgcttc ccgttcataa gtacttcaag cgtcaccggg
      121 aagtaacaat cagcgaaacg cggattatga taaccgtttt gttcaattaa attaaacacc
      181 tctttacttg cgacaatccg catattaagt tcagtttcag gatgccaaaa ttctaaataa
      241 gcattagtcg gtgcgacatg ggcatcaagc ggataagtaa agccattagg aaagggacgc
      301 acaatataat cttcatcagt gtaagtaatt tcgggatcaa gcggataagt tgttttaaaa
      361 ttagtaaggt ccgctggtgt aatatcacta ggcgtttctt cactactagg gtcactaacg
      421 gaagagagca cttcacttga taactcttct tcgctagaag gcacttcttc actagttact
      481 ttttcttcgc tactttcggt gtcactaaca ctatcgctag tcggcgagtc actacttcta
      541 gtcgttgaac ttaatacttc actaatcgtt gtgcaactac ttaaaagcat catgagtact
      601 attaataaaa gaaatttacg gtttttcata atgactaact cctataataa aatcattttt
      661 attataaact aaatacccgc attttgcaaa ttttagtcga aaaaggagcg attttcgtcg
      721 ttccctaagt gttttgttaa attcgtttaa ttatgcaacg tttgcttttt tacgaaaacg
      781 aacaaacccg aatgcggcaa ttgcaaataa cataccaatt actaacacat aaataatatt
      841 attattagca actttgttaa tacggaattg ttctggtgat ggatgtgcag catcatataa
      901 tgatttcatc attacgtatt ttgcataagc atcagtggct ggagttgctt cacgaacacc
      961 agtggcgtaa tcttcttcac ttgtgtagtc agcaatttta acaccatgtt ctccacctaa
     1021 atcaccttgc atatcccagt gttcaccagc gcgtggtaag aagttggtat ccactaaatt
     1081 gaatgcttca taaccattat cagcactagt ggaacaagtt aaataccctt ctaatacttt
     1141 cgcaaagaat gtattgtaaa tgcgtccttc aacagcgccc tttgtaactg agtaagtttc
     1201 atccatattt tgtttaacaa cccataattt gctattgtct tgagcggcat atggttcacc
     1261 agctactgcc acttggaagt ataggtttgt atcaacaatc acaagatcaa catttttacc
     1321 ggtaagtgct gatagtggta catcaaagta cgcaaaataa acatcatcag taactaatgc
     1381 tttttcgtac cctgtagcgg tatagtggac atcgccagct tttagtaaat attgataccc
     1441 ttctgtcgca taatgaccac cacggttcac ccaaatccga accttttcac tactaattcc
     1501 agcagttgtt ggggtttgtg agactaatga tcctaaagtc ttccaagcac ctaaagtagt
     1561 gtcatacata tcttttgtgc cgtcataaat taggtctggg gtttgtttaa gtacattagt
     1621 taatccatcc acttcccatg catttgtagc ggattttaaa ctaacaaaaa tgactttttc
     1681 ccatccgcct ggtgcagctg gtattggtgc ggtaacttta taaacacctg tttgatgttc
     1741 aacaaggtta acgaattgat tttctccacc aacaaagaaa tatgctgcta agtggtcata
     1801 accttcagca aaatatgatg catcagggcg gaagtataac actgtatcgc cagcgattgc
     1861 aggattaatg tgttcagaag caacgaatgc ttcgggttcc cccacggtcc aaccagtcac
     1921 tcttacaagt tcttttgttg catcatatac taggtctccg ctttgattcc attttgaatc
     1981 ccaatcattt tctggtttag cgggatccat ccgaaccatg atgaactttg cgtgattgcc
     2041 agctggctta acgacttcat agaggtcatc agcgtatagc gtacgttgca tggttacaaa
     2101 taaatcagga tcagttcctt caaaaaaata ggtctcaaaa cgagcaccat cggatttcca
     2161 tccttcactg acctttaagt aaattttagc aggtaatgct gctgccttta ctggtgcttt
     2221 agcgtttaga cttacgccaa cactaaaagc catcgctaat gttgttaaaa acattaaaaa
     2281 gcgattttta aaatgattct ttttcataag ttctcctcca acttacatat ttaaacatga
     2341 taatactgta actttaatat gtatctaagt caaatgaggc ttaaataaaa aaagaaaagc
     2401 caagaatgga tcttggctaa tctattgaat aatttttagc gttaaatttt agtaactaag
     2461 acactccacg ctggtagtgt gtttttagta gcaccttcta gcgataaaat gacagtacct
     2521 ggtaagttaa gacttgttaa ccca
//
LOCUS       DAOSLP010000068         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_60821_length_1710_cov_6.003021, whole genome shotgun sequence.
ACCESSION   DAOSLP010000068 DAOSLP010000000
VERSION     DAOSLP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60821_length_1710_cov_6.003021"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..234
                     /locus_tag="PK460_04925"
     tRNA            158..234
                     /locus_tag="PK460_04925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Arg,seq:tct)
     gene            422..1171
                     /locus_tag="PK460_04930"
     CDS             422..1171
                     /locus_tag="PK460_04930"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOE54448.1"
                     /translation="MKDLATLVKEFKTIIDNSNNVVFFGGAGVSVPSGIPDFRSADGV
                     YSKDTNTMYSAEEMVSRTMYYRNTPFFNDFYFKYLVYPDALPNPCHTYLARLEKEGKL
                     KAVVTQNIDGLHQAAGSKVVYELHGSVHRNYCTLCKAFHDLAATLKEPRGKCKKCGGY
                     LKPDVVLFEEGLDQNTITRSIEAIAKADTLIIGGTSLVVQPAASFINFFRGKNIVVVN
                     KTSIRLPMYLSTNILFIEADIGKTFELLNKL"
     gene            1223..1378
                     /locus_tag="PK460_04935"
     CDS             1223..1378
                     /locus_tag="PK460_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54449.1"
                     /translation="MLNKVINDIASLVEVSYDKSTTIALVLFIGVMGLTIITLYYFLT
                     KNNKKKK"
BASE COUNT          602 a          262 c          296 g          550 t
ORIGIN      
        1 ccaatgacaa ttcaacttaa aaagtattac gaacctcaaa atatattaat atacgctaaa
       61 gaactcacac gactttaaaa gctaaattta acaataaaac tattttttaa tgtcacttgt
      121 tgaaactaat attcttttat tgtaaaataa cttgcgtgtc ccggtagctc agtcggatag
      181 agcaacagcc ttctaagctg tgggtcgggc gttcgagtcg ctcccgggac gccattaatt
      241 aatgaagaag agttagtgtt taccccctag atgctgactc tttttttcta acattaaatt
      301 gaactagttt tgtgataaaa acataacata aaggctaatg gtaattaatt agtaaataat
      361 tagtagaata tttagtagag aaagcattaa atagcttatt cttaagtgtt ataataaaag
      421 tatgaaagat ttagcaacat tagtgaaaga gtttaagact attattgata atagtaataa
      481 tgttgtcttt tttggtggcg ctggtgtgag tgtcccgagc ggtattcctg attttagaag
      541 tgccgacgga gtgtatagta aagacacgaa tacaatgtac tcagcagaag aaatggtgtc
      601 acgcacgatg tattaccgta atactccttt ctttaacgac ttttatttta agtatcttgt
      661 ctatcccgat gcgcttccta atccgtgcca cacttattta gctagacttg aaaaagaagg
      721 taaattaaaa gcagtagtga cacaaaatat tgacggttta catcaagccg ctggttcaaa
      781 agtcgtctat gaacttcacg gcagtgtgca ccgtaattac tgtactttat gcaaagcatt
      841 tcatgattta gcggcaacac taaaagagcc gcgtggtaaa tgtaaaaagt gcggcggtta
      901 tcttaaacct gatgttgtgt tatttgaaga aggactagat caaaacacga ttacgcgttc
      961 aattgaagca atagcaaaag ctgatacttt aatcatcggt ggcactagtt tagtcgtgca
     1021 acccgctgcg agttttatta atttctttcg gggtaaaaac atcgttgttg tcaataaaac
     1081 aagtattaga ttaccaatgt acttaagcac taatatactt tttattgaag ctgatattgg
     1141 taagaccttt gaattactaa acaagttata aaaagcaatc gtataatcgt gctattatat
     1201 taagcaagag aaagaatcat aaatgttaaa caaagttatt aatgatattg cttccctcgt
     1261 tgaagtaagt tacgacaaaa gcaccactat tgccttagtg ttatttattg gtgtaatggg
     1321 tttaacgatt attacacttt actatttcct aacaaagaac aataaaaaga agaagtaaca
     1381 cttctttatc aataattatg taaacaaaaa aattaggtgt ttattattaa acacttaacc
     1441 acttttttac aaataaaaat tatgaactta tcatcaaagt tttaatgctt tcttaataat
     1501 tcattattaa ttcgctcctt gcaaagtttt attattttgt ctaatatgta tactagatat
     1561 atatgtgcgt aatatcctaa aaaaataata aagaggaaat aatatgcgca aaataattaa
     1621 aaaggcatta ggaccattag ccatttttgc aatggcacta attgtcggtc ttagtgtggc
     1681 aaaagaaaag acacctgtag gggtgaaagc
//
LOCUS       DAOSLP010000069         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1620
            NODE_61853_length_1691_cov_9.329462, whole genome shotgun sequence.
ACCESSION   DAOSLP010000069 DAOSLP010000000
VERSION     DAOSLP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863149
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:47:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 990
            CDSs (total)                      :: 943
            Genes (coding)                    :: 933
            CDSs (with protein)               :: 933
            Genes (RNA)                       :: 47
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 43
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61853_length_1691_cov_9.329462"
                     /isolate="AMR_MDS_1620"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..77)
                     /locus_tag="PK460_04940"
     tRNA            complement(1..77)
                     /locus_tag="PK460_04940"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:cgt)
     gene            160..903
                     /locus_tag="PK460_04945"
     CDS             160..903
                     /locus_tag="PK460_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54450.1"
                     /translation="METKKKRTINIILKISIIVAAIIGIISQQLHGLLIYTGHVPFMY
                     FTTQSNILVAVCMGILLYYDLTEKEVPRWVLIFQHITVTAVTLTFLVFAIFLGPFIPD
                     LSVGYFYSVQNLTLHNIVPILAISQYLYDDNRDIPKYAKYLGLSSGFIYVIFVYIVHL
                     CGATFGATEFPYFFLDYKEFGWLTINEGKIGIIYWWIVIILLLLGISALLYYLKKKSA
                     ERKNIPVAIFNSLIILSIIFITINIIIKI"
     gene            926..>1691
                     /locus_tag="PK460_04950"
     CDS             926..>1691
                     /locus_tag="PK460_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE54451.1"
                     /translation="MKLRKLKAVPLALVTLFTLTSCKLIDNIIGSILVNTVMDLPVLI
                     NRLDLMPSAENTNPTFVPFENPPSSEANRAELNYLKNVLVVPRDLETVVFNKTIKLHF
                     DFEFSGEGATNAFHVIDVTESDLPENFRFDTSAFPVPITYKLALIVPVGESTILEGVT
                     NINQIQTVLEDTPRDELVELIDQEPLNATMKVIARSAGKSKSKNFYFNLTKPLFGAMV
                     LEEVFKSGYLINLYRIHGFNQENNEVESTIENVADLP"
BASE COUNT          534 a          297 c          282 g          578 t
ORIGIN      
        1 tggtgccgac gacaggaatt gaacccgcaa cctactgatt acgattcagt tgctctgcca
       61 gattgagcta cgtcggccta gtcttaatca tatcgacttt atcacaatat tgcaataaaa
      121 acatcaagaa aatatcttga tttgtagtat aatcttttta tggaaactaa gaaaaaacgc
      181 actattaaca ttatcttaaa gattagtatt atcgttgccg caattatcgg aataatatca
      241 cagcaactgc atggtttatt aatttacacg ggtcacgtac catttatgta ctttacaacc
      301 caaagtaata ttcttgtcgc cgtttgtatg ggaattttac tatattacga cttaactgaa
      361 aaggaagttc cgcgctgggt tttaatcttt cagcatatta cagttaccgc tgttacatta
      421 acctttttag tgtttgctat tttccttggt ccatttattc ctgatcttag cgttggttat
      481 ttttattccg tgcaaaattt aacattacat aatattgtcc cgattcttgc tatcagccaa
      541 tatttatatg atgataatcg tgatattcca aaatatgcta aatatctagg cctttctagc
      601 ggatttattt atgtaatttt tgtgtatatt gttcaccttt gtggcgctac tttcggcgca
      661 acagagtttc cttacttctt ccttgactat aaagagtttg gatggttaac aataaatgaa
      721 ggtaaaatcg gcattattta ttggtggata gtaataattt tattactact tgggatcagt
      781 gcactcttat attacttaaa gaaaaaaagt gctgaacgca aaaatattcc ggttgcaatt
      841 tttaatagtc taattatttt atctatcatt tttataacaa ttaatattat aattaaaata
      901 taaagaaggt ttagggggtt ctattatgaa acttcgcaaa ttaaaagcag tcccattggc
      961 gcttgtcacc ttatttactc ttactagctg taagctaatt gataatatca tcggatcaat
     1021 tcttgtcaat accgtgatgg atttacctgt gcttattaat cgccttgatt taatgccaag
     1081 tgcagaaaac actaatccaa catttgtgcc atttgaaaat ccaccaagta gtgaggctaa
     1141 tcgcgctgaa cttaactatt taaaaaatgt attagtcgtc ccgcgcgatc ttgaaacggt
     1201 cgtctttaat aaaacgatta aactccattt tgattttgag tttagtggtg aaggagcaac
     1261 aaatgccttc catgtcattg atgtaacgga aagtgactta ccggaaaact tccgctttga
     1321 tacaagcgct ttcccagtac ccatcaccta caaattagcg ctgattgttc ctgttggcga
     1381 aagtacgatt cttgaaggtg ttactaatat caatcaaatt caaacggttt tagaagatac
     1441 accacgtgat gagttagtag aattgattga ccaagaacca cttaacgcca cgatgaaagt
     1501 cattgcgcgc tccgctggta aaagtaaaag caaaaatttc tattttaatt taactaaacc
     1561 attatttgga gcaatggtac ttgaagaagt atttaaatct ggttacttaa ttaatcttta
     1621 tcgtattcac ggttttaacc aagaaaataa tgaagttgaa agtacaatcg aaaatgtcgc
     1681 agacttacca a
//
