LOCUS       DAOSMC010000001        69983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_500_length_69983_cov_17.383223, whole genome shotgun sequence.
ACCESSION   DAOSMC010000001 DAOSMC010000000
VERSION     DAOSMC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 69983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69983
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_69983_cov_17.383223"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PL075_00005"
     CDS             <1..491
                     /locus_tag="PL075_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOD96627.1"
                     /translation="IIIILVVVFYKPGSQTTLAPTATPGIGNTSPTKGEVPKDAKVYE
                     VGETAPEGVAVPLSVSDYATGKLRYYETKIENDVFTPSTFSCYEGDLLKIAITAVDKD
                     ADIVIPDMNMAFPLIKKGETKTLEGQMLNPGKYTFYCEKCGGLNSTAVGYIIVVPKSS
                     EE"
     gene            932..1531
                     /locus_tag="PL075_00010"
     CDS             932..1531
                     /locus_tag="PL075_00010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOD96628.1"
                     /translation="MKRQKRNQRKSSEIEKLKKELSLYKKLALKDPLTGLYNRRGFLE
                     ETDRLIKMNQRLGQRFSFSLASFGRVGLVFIDLDNFKHINDRFGHKVGDEVLKEIAKR
                     LEGSLRRFDIVGRWGGDEFVVAIWDVDKKTLDIIVERLKSKITNEPIIIRKQPISITA
                     SFGAKVFPIEGLELEKEIEGVDKSMYRAKKQSKNRLTSA"
     gene            1858..2286
                     /locus_tag="PL075_00015"
     CDS             1858..2286
                     /locus_tag="PL075_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOD96629.1"
                     /translation="MNKKLSTNKIISLIPSPSDFSSNQEWENACWEKISNQPEVFNFL
                     LTPAERRKIIKRLAVIKELQAGKSYRMIGVELNLSLQTIHNIKKIMNDEGIYYSYFSR
                     VRKIKQKEKEQLLNTRKIKIARPEVGRRYRRTKFGKVRLV"
     gene            2997..4472
                     /locus_tag="PL075_00020"
     CDS             2997..4472
                     /locus_tag="PL075_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96630.1"
                     /translation="MHKNIRRRIFILASCIFVLAALLLVLYSFGYRWDKEKFQPVLTG
                     GLYLRVNTVKPQIYLDGKFLSKATPGLLSSGIFLPNLLPDTYQLEIKKDNYFEWEKKV
                     DIKSQQVTSFSSIVLLPKTPLVTDIYKITVDNATNTKTKFQEEIIDFLPLANGKEIII
                     QTKEVSTSTNAVLKIYNFIDGSSVEIYRQKLAKNETLNLIPRLKINDDEANEVIFDLT
                     KNNIQIFYLWQRIEPERIQSLSQQITTTFKLKNPIQKIEFYPNIEGKYIVLSGKDLMV
                     VDFTKNETKKIISDIVDFQSKDYVLFWLEQSGAIFSYNLILGFSTPLGIIELPDKFSV
                     TRMEIAPNSQCFALLLDNNEWYFFRAGQSMEKINDVVKFVFAPDSKKVVYLTKTGDVR
                     MRFLSDIAGDVIRKKGDEVLIRANVSSSADLEWYADSQHLIVAEGKIIYFSEIDDRDQ
                     INTFIQEFEFDHYLLTNKKSGTVWGMRPDLIQKIDLVYQKV"
     gene            4758..6437
                     /locus_tag="PL075_00025"
     CDS             4758..6437
                     /locus_tag="PL075_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOD96631.1"
                     /translation="MADQDEAKKLSHILEGTREAAEEREAERLAKSLKFQYANLTKFA
                     SQTQALQLINEEEARAALCAPFNVQQKKVFLACKNPALDATKNVIDSLKQRGYEVEIF
                     VVSSKSLETLWKGYKFITGRKKEITGGLEIDANRLAELSSEIKNIAILKEKIDKFDSP
                     YISQIFEIIIAGALTMDASDVHIEPEESGVRLRYRLDGVLQDIVDLNEHYYRSILSRI
                     KLLGGMKINIHTAPQDGRFTIKTKDLEIEVRVSIIPSAYGETVVMRLLDPRRIQLDLK
                     ELGFREDDLVIIEEQVARPNGLILNTGPTGSGKTTTLYAFLRRIYQPGIKIITIEDPI
                     EYHLEGITQTQVNPTRDYTFANGLRSILRQDPDVILVGEIRDNETAEIAMHAALTGHL
                     VLSTLHTNDACGTIPRLLDMKVQPAILAPALNLAIAQRLVRKLCVHCREKVEATAEQA
                     EKIRKVIERLPDRVKKPTLGKQITFYKSKGCELCNGTGYKGRIAIAELLVVDREIQDL
                     INQGDRMPTANEIKDLAIKKGMTTMEDDAILKILDGITSLSEAENVLGKLL"
     gene            7320..8333
                     /gene="ruvB"
                     /locus_tag="PL075_00030"
     CDS             7320..8333
                     /gene="ruvB"
                     /locus_tag="PL075_00030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOD96632.1"
                     /translation="MSKNIFKSDPLLDNALRPKRWEDFIGQEKVKKNLRIMLGAAQKR
                     EEVVDHLLFYGGAGLGKTTLAYLIAEEIHANLKITSGPAIEKAADLASLLANLEEGDI
                     LFIDEIHRLNKMIEEILYSAMESRVLNLVLGKRSSTRAVQLDLPPFTLIGATTRIGLL
                     SSPLRSRFGAIFKLDFYDLEDIKKILQNNARLLGVNISEEAMEILARASRATPRVANR
                     LLKRSRDLATIEGKSEIDAAISRKILELFDIDEWGLEATDRKILEVIVDRFNGGPVGI
                     KALAAATNEEEDTIEEIYEPYLMRLGLLERTTRGRMVSDAGYKHLNLYHLAQKGEQKK
                     LID"
     gene            8465..9802
                     /locus_tag="PL075_00035"
     CDS             8465..9802
                     /locus_tag="PL075_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOD96633.1"
                     /translation="MFKHYFSFLIVALSVLLFFPLVQALAVGPVIINEIMYDLQGTDT
                     DHEWVEIKNISDQAVDLKDWRFYDGSNHLLNEPPQNGGQGSLIIAPGGYAVLADKADV
                     FLADHPGFGGIVIDTVMSLNNTEETVQLIDQSGNSIEKINYQKEMGGNGNGYSLERTG
                     DFSMQFCESNQLGGSPGQANNFDCNKSSFMPTPSLTAMNTPTLMMATVSPSASPETIL
                     EDTIIESEDNLLRTTPIASSPVIKVSIIINEFLPNPTGSDTDNEWIELYNNSDVKVAL
                     TDWWIEDASGQKYTFKDEEIMSQGYLTLPYSKTKITLNNNGETLSLYSPRGELAFKIS
                     YSGKANEGMSFARAGQNDWRWTSILTPNKQNQFSSESSQNTELAIQQMDESDVAENNA
                     PSTVDADSSLKQQTVTSSISNASDKIIIVAIGLGLILSVVAAIFIKKILPPVH"
     gene            complement(9810..12362)
                     /locus_tag="PL075_00040"
     CDS             complement(9810..12362)
                     /locus_tag="PL075_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOD96634.1"
                     /translation="MTLILIDSHALIHRCFHALPNLTTPKGEPIGAVYGTIRIILKII
                     REFKPDYLAACFDLAEPTFRHKEYKEYKATRPKTDENLIPQFDKVREFFSAVPIKYFE
                     IPGYEADDLIGTLVKKFSNEKDLKIIILTGDLDTLQLVDDDKVVVYTMKKGIQDTVIY
                     NEKEVQERYGLSPKQLIDFKALKGDPSDNILGVKGIGEKTAIELIKHFETLENLYQKI
                     ENENFQKTSEARLIKPKTLAALLDQKEQAFFSKYLTTLNCNAPLEVKLSDLKFNGINK
                     ESLIPLFKKWRFESLVNQLMPEESQTNTPENAFSQASIQWQTEQKLNPLPTQAEAIIN
                     LNKNVELLVADQFFSLPKDQNSFNKIKQFSLVGYDLKNIVKQIDDYNLNLNHDIKIAQ
                     WLINSELKDYSLQRIWQIIFKTPLPNDNILTILKKIKTAQVNKIKELALTNVFQQIEM
                     PLVNVLAQMEHFGIKINPDHFYRLTDELNHELQKLEKEIFELTNSEFNINSPKQLSQV
                     IFQKLNLNNKHLSKTATGLLSTRENELIKIKDLHPAIAKILQYRELMKLKSTYLEPLP
                     SLMDENCRIHTTYNQTGTATGRLSSENPNLQNIPIEGELAKKIRQGFVAEKGYQFVGF
                     DYSQIELRIAASLADDPKMKEAFLNDQDIHALTAAEINNTTLDQVTPAMRRQAKTLNF
                     GILYGMGAKAFSETAQIPKDQAKKFIEEYYHDFANIKDWREKTLAEARQTGCVKTLSG
                     RIRWVWDLISGNQKYRSLAERAAINFPIQGLAADIIKKAMIKINDFLQKENAIDDIRL
                     ILQIHDELIFEVKENKVEWTINNLLPLMEQNEFLSVPLKAQYYTGETLADLK"
     gene            complement(12402..12908)
                     /gene="tsaE"
                     /locus_tag="PL075_00045"
     CDS             complement(12402..12908)
                     /gene="tsaE"
                     /locus_tag="PL075_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOD96635.1"
                     /translation="MQSSLKILSHSPVQTQKIAQILVKTLLDQKSSPPHIFALQGNLG
                     AGKTTFIQGVGKSLKIKGRILSPTFLIIKSFSLRKNPHFKNFYHIDCYRLEKAEEILT
                     LDFKKIIQDPKNLVFIEWPERIKKLLPKNTLWINFKVVDKNKREITINSKVENSKIKS
                     QPKTKKLK"
     gene            13078..14358
                     /gene="murA"
                     /locus_tag="PL075_00050"
     CDS             13078..14358
                     /gene="murA"
                     /locus_tag="PL075_00050"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOD96636.1"
                     /translation="MSHFFEIEGSKKLKGEIKVSGTKNGALAILPACLLTEEPCVIKN
                     IPLLTDVLVMVDILKNLGAEIEWLDERTIRIQAKNVLSDADTKLVRKIRASFLVAGPI
                     LARIGELKIAKPGGCHIGARSLDTHFEGFADLGVKSALEREESEKYYLKAPKQFKPAI
                     VSLNEFSVTATENLMMLMAKIEGKSEIHLAAAEPHVQELGEFLKSMGAEIEGLGTHTI
                     IIKGKQKLKGAEHTIWPDYIEAGTFFSLAATTKSNLTITNVPVAYLEMVFRKLKKMGV
                     SYRIKDSQVLIEGKDSSLKAAKIQTLPYPGFPTDLQAPFAVLATQAKGDSLIFDTLFE
                     GRFKYVDELNQMGAKIFLCDPHRILVSGPTPLYGTKIKSFDLRSGATLIIAALAARGK
                     SQLLGIEEVDRGYEKIEERLQKIGANIKRVEAES"
     gene            14363..15370
                     /locus_tag="PL075_00055"
     CDS             14363..15370
                     /locus_tag="PL075_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD96637.1"
                     /translation="MFIKKIGIDLGTTNTLVFVPKKGVIIFEPTIVAYDLNDNKILAV
                     GKEAKEMLGRTPDDIGAYRPLKDGVIADYKTTKIILKYFISKALGSFNFFKPTLIISG
                     PAGITSTERRAVINAALEAGAREVYLVREPILAALGAGIPINSASGHMIVNIGGGTSE
                     IAVISLGGIVSWASLRIAGNKFDEAIVNYIRKKYNLAIGDQTAERIKIEAGAALPQKN
                     KIETEAKGRDLTTGLPRSVIVNSNEIAEALAPHLEEIAESIRSVFLNTPPELAADIIE
                     KGIVIAGGSSLLRDLDSYISKVTGVNTFLAEDALYCVAKGTGIILEHLDIYKRALMSK
                     R"
     gene            15372..16121
                     /locus_tag="PL075_00060"
     CDS             15372..16121
                     /locus_tag="PL075_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96638.1"
                     /translation="MKIDQYFKKIIEQDIIDGAWLFLSMDEEMLFQKSLSLAKQVTSA
                     SYIYIVSESEQLEDFIKNSHQVFDPAIKSIFEIQRLLFLSSDEKKVVIVKMMEDLSEE
                     AQNSLLKITEEPPVNTAFFFLSKDENRVLPTLRSRFRIIKFPEKNSFYQKRISSEMRT
                     FLHSSQPLNVYLENFLEEDPVIFLENLVIVMRDGLLNSLNLEELKITNGEGEANVKDL
                     KNALYALEGIKYHNLNSRLQIEDLLLNINKN"
     gene            16469..17032
                     /locus_tag="PL075_00065"
     CDS             16469..17032
                     /locus_tag="PL075_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_process: GO:0002184 - cytoplasmic translational
                     termination [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HOD96639.1"
                     /translation="MPINQLIEKLKKEGESVIEGFRNEILGVRSNRPQPALIENIKVE
                     CPVYGSILPLKQLATIQVSLPNSLIVQVWDKVNVSACEKAIQASNLGVSTIVEGSQIR
                     VILPPLSQERRDQIFQLIKKKSEDTKIRLRLLREDVLKEIKELFEQKKISEDDKYRSK
                     EQIDKLMDDFNHKIEEIVEVKKKEIYE"
     gene            17073..18149
                     /gene="rseP"
                     /locus_tag="PL075_00070"
     CDS             17073..18149
                     /gene="rseP"
                     /locus_tag="PL075_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOD96640.1"
                     /translation="MSAIIFILCLAFLIIVHEFGHFLAAKKSGMKVEEFGVGLPPRIW
                     GTKKGETIYSINALPFGGFVKIYGEDGSYENEENSFSAKKPAVRLKVLLAGIAMNLIV
                     GIILLGVGFNIGIPALATEDNMPYLQDIKLSILEVQKDSPAAMAGLKTGDRILEVSRG
                     DEKISLPTVEELQSFTKKWGGEEINLKVERGKESFEIKATPRKNFEENQGALGIAIGE
                     TGILKYPFFQSIWEAIKGGFSIFINVFIGLFLFLKNLIINGQMMGEVAGPVGIVALGG
                     QTVKMGIGYLIQFLATLSIYLAAFNLIPFPALDGSRALFVLIEKIRGKAVPAKVENLI
                     HSLGFVILIGLVIVVTFHDILNLF"
     gene            18197..19468
                     /locus_tag="PL075_00075"
     CDS             18197..19468
                     /locus_tag="PL075_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOD96641.1"
                     /translation="MLESKAFVSINRGAVEDETALNAQLLIRGGFVEKVMPGIYVYLP
                     LGWRVYKKIETIIRTEMFRIGSEEIFLSALLPKELWIKSGRWESFGDILMKVNDRDGR
                     EYVLGPTHEELIAELAKKYILTYRDLPKAIFQIQEKFRDEQEVQSGLLKTKEFIMKDL
                     YSFHATRQDLDFFYEEMKKSYINIFNQCGLKSYIVEAAGGNFTDENVHEFMLKTEAGE
                     ETTVFCELCDFAQNKQLKEAEGRDQCPRCGGLLKEEKTVELGNIFKLGTKFSENTGAY
                     FVDRDGEKKPILMGCYGIGLSRLMGVIAEVYHDEKGLVWPVSVAPCLIHLIKINETDP
                     KIDAKIKKQADKLYQQLTRKGFEVLYDDRVEFTSGEKIAEADAIGIPYQIIITAKNLQ
                     RELVEIKSRNSGESELLKISAVGNYFKNINQ"
     gene            19471..20523
                     /locus_tag="PL075_00080"
     CDS             19471..20523
                     /locus_tag="PL075_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOD96642.1"
                     /translation="MFKKLLAKMSPGLGIDLGTANSLVYLEKQGVVINEPSVVAYNQK
                     TNEILAIGREAQAMVGRTPPHIVAVQPLTNGVISDFELSEEMLRYFYRKFQKSKLGIL
                     PYRPRVVIGIPCGITEVEKKAVEDAALNAGAGEVYLVEQTLAAAIGSHIPIFEPSGNI
                     LVDIGGGTTDIVVISLGGIVRYKNLKIAGQKFNEDIIRYVKDEYRLLIGEVSAERVKL
                     EIGSAKINGMEKLETVVRGRDLTTGLPKEVTLTSEEVARALDKSLKVLVEAIRSTIEE
                     TPPELVADISIKNIWLSGGGSLLHNLDRLIENYCMMPVKSVDDPLTATVRGLGIIVEN
                     LKQYKNILIYEHKENE"
     gene            20501..21310
                     /gene="mreC"
                     /locus_tag="PL075_00085"
     CDS             20501..21310
                     /gene="mreC"
                     /locus_tag="PL075_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOD96643.1"
                     /translation="MSTKRTNNLIVILLIIAFLGSFMRPVLALATNEEDSNNGDKTFT
                     ARARTILLRAVQDIDNWVRGLIYFKEIQEENNVLVFQNRALKSLLIDYHNLKSENELL
                     REALKIHQSQDINFLLANVIGRSPLNFSQTFVIDVGEEDGIKVGQSVVWGGKTLIGEI
                     IDVRKNSSTVRAITDSEFRAAVFVGEGRTEALFTGNGFLPPFLDLVPTKEEIALNEQV
                     YTSGLDNKFIRGLYVGDVSEINKPEGKIFQEIKITPALDWTSIYQVLVIVH"
     gene            21313..21828
                     /locus_tag="PL075_00090"
     CDS             21313..21828
                     /locus_tag="PL075_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96644.1"
                     /translation="MKKIKQILHVLTFLLFISLLAILQITIINQYPIGGVKVNLVLIG
                     LLANIFNPRFGKVVLGAVVGGLIYDFGGIFNFISLISFLVTIVILHLWSKKYLVKKNM
                     LMIFFFGAIGTIFFNFFYVLLSYFLFHANFFYYFLSLRHVVEILLNGGGAFIFALIGE
                     GFTQISHLFKK"
     gene            21828..23753
                     /gene="mrdA"
                     /locus_tag="PL075_00095"
     CDS             21828..23753
                     /gene="mrdA"
                     /locus_tag="PL075_00095"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOD96645.1"
                     /translation="MKLNFKKNKKVKRESDFLEVEEILFNKEAVSKLKEEELSRLEVS
                     IGKWGLNSIFLFGIFLLIFALGLVGYLNFAKGDEYQQIAQKNALKSIPIIADRGLIFD
                     NHNRVLVYNQEVFDLVFFPAYLPVDKIEREKILKKIEEKPEIAAAINKENLEEDDPLE
                     SIILQSNLSREEAIRWESYFAGYSAVQVIRQNLRQYTSPLAFSHILGYVGRVSAEDIQ
                     KNPLYSNFVKIGKAGLESFYEDNLRGQDGEIKVLRNAYMEILGQSVVKEPVAGNNLYL
                     TIDADLQEYSYKTLARQIDNLGKERGGVVIVSDPQSGEILSLVSYPGYDINSLSKGIT
                     QKEFETLINSKVTPLFNRTVSGLYNPGSTIKPIIAIAALEENIIDPFKKIETHGYITI
                     PNPYFPDRPSIFVDWRNNGVVNMQDAIARSSNVYFYIIGGGYEDFVGLGIERIRNYLE
                     KFQFNLVTGIDLPAEKVSNIPDEKSKNPWRIGDTYNVSIGQGDLLTTPIRLITSLNVM
                     INGGKILRPYLLDHIADNNQRTIFQNEIQVAKDNFLNSKNIEIVKEGMRKTVTSPLGT
                     AHTLIDLPFSVGGKSGSAQTSGNTKTNALFYAFAPYDDPQISVLILIEDVPEGSLNAI
                     PVAKDILMWYYKNRGFK"
     gene            23997..24461
                     /gene="greA"
                     /locus_tag="PL075_00100"
     CDS             23997..24461
                     /gene="greA"
                     /locus_tag="PL075_00100"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOD96646.1"
                     /translation="MPPVYLSKEKYEELCRELEARKTTIRQKIAADLKHAKDLGDLSE
                     NAAYTEAKDAKEKNDRRIDELENILAHCEIIEKSQKNDRVYIGAKVKIQDEKGEIFEY
                     QIVGPEESDPFKNLISYRSPLGMELMDKKEGDWIKIQSPNQTIKKYKIISID"
     gene            24466..25944
                     /gene="lysS"
                     /locus_tag="PL075_00105"
     CDS             24466..25944
                     /gene="lysS"
                     /locus_tag="PL075_00105"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOD96647.1"
                     /translation="MSTADQVKKIRIAKIKELKKQGKEVYPSHCFRNQSISNILNNFY
                     SWSIAKKKIWVGGRIMSIRFHGGIAFADLWDASGHLQIVFKKEETKNFELLNQYLDVS
                     DFIEVRGFPFKTERGEKSLLVQEWRILAKSLKPIPSEWYGLKDTEERFRRRYLDLILN
                     EEVKKRFETRSRIIKAMRGFFDKEGFMEVETPILQTLAGGAAACPFKTKLNILNMPLY
                     LRIAPELYLKRLIVGGFEKIFEIGKNFRNEGIDKTHNPEFTMMELYWAYADYEDMMDF
                     IEQLFVYILKNALPQTKDPMLINFNGQLINFTPPWPRKKFLDLIEEETSLKPLATKSQ
                     TWRAFLQTQGVTITEEIKKMDEWGLMDEVYKKVCLEKLTQPTFVTHHPIVLSPLAKLS
                     KDNLNETERFQLVAGGIELVNGYSELNDPLEQAERFKKQEQRRKAGNKEASPYDKDFI
                     EALEYGMPPTAGVGIGIDRLVMLLTNAPSIKEVIFFPFMKNK"
     gene            25949..27181
                     /gene="serS"
                     /locus_tag="PL075_00110"
     CDS             25949..27181
                     /gene="serS"
                     /locus_tag="PL075_00110"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOD96648.1"
                     /translation="MLTDLLKKIRENPQLYKEGIAKKNCNVEIIDKILAIDKEYRSNL
                     AEVEFLRAHLNELSQNAKFSQDHLEQARATKQRIKDLERELTPIENDLDKLLYQIPNL
                     PFEDVPLGKDESENKVLREVGKRPKFKFEPQDYLTLTEDKWIDTKRAAKVAGTRFGYL
                     KNEAVLLEYALVRWVMNSLKEKGFVPIVPPAFISSKAMKGMGYIDNEKDLAERYYFPQ
                     DDLFLIGTAEQAVGPMHMDEVLEEKELPLRYIAFSPCFRREAGSYGKDTKGIWRVHYF
                     EKLEMFIFCKPEQSEEEHQLILAIEEELMQKLGLPYRVIQLCTGDLSNPSAKSFDIEA
                     WLPSQNRYAETHSCSNCTDFQSRRLNIRYRSKDGLQFVHTLNGTAFAIGRIIVAIIEN
                     YQQADGSIKIPEVLEQYL"
     gene            27197..28000
                     /locus_tag="PL075_00115"
     CDS             27197..28000
                     /locus_tag="PL075_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96649.1"
                     /translation="MPYRLTSYKLRQIQKKKQRRRTIFVIIIIVLFICCLGGILFFSP
                     LFQINSIAINSTHFVKMSDIFQKATEQFKDQKLFNLITVPANLILFTQADCKAIEKYF
                     VALDSVDCQKIYIAKMLKIEAREKQQIGILCSDSVIKNHGNCLYFDQNGIVFALAEED
                     DDFDHSLIAMQDVTNRIYQLGDKIISYDFSPVVKIKDILANHQLILNKIEIDDHDVVY
                     FFENGFKLLISQEKLKETANILPRLLEIEFDLQKLEYLDLRFLPKMYYK"
     gene            complement(28087..28824)
                     /locus_tag="PL075_00120"
     CDS             complement(28087..28824)
                     /locus_tag="PL075_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOD96650.1"
                     /translation="MNKIIKIKNLIYDCLFPIFCLNCGAFIKNESGLYLCENCLKTIP
                     LYSSLFCPICLKRIGQFKKCSHSNNYSPLDFIASATDYENPLVQKLIHLYKYQFVKDI
                     RHTLGNIVINYWEKIWPDLKKQFSGAIVIAIPLSRQRFKERGFNQSEEIAKIFANYFQ
                     LEINSEILIRQKNTHPQAKLDNYEERHLNLKNAFEVKDKNSLKNKNIILIDDVFTSGA
                     TMQEAARVLKQNGSKKIIGLTVAKSRK"
     gene            28910..29950
                     /gene="pilM"
                     /locus_tag="PL075_00125"
     CDS             28910..29950
                     /gene="pilM"
                     /locus_tag="PL075_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOD96651.1"
                     /translation="MRTLGIDIGTTNIKAVELSGTLNNIILENYGILETYGYLERSNA
                     AIQTNYFKLVEEITTELLRQLFSAFKPKTKRAFFSLPVFSSFITNFDLPFTNNKEIVQ
                     AVPFEAKKYIPLPLEELEINWMVIAKAADGRKEKSRIVLVATPKELIQKYQHIAQTLG
                     LNLMGWEIEGFSLARTLVADDKTPTLIVDIGTQVTNLFVVDNTFLVNFESLNIGGAEI
                     THIISQSLGVTQERAEEFKKIKGFKVNPEEMGVVNVLMPIVDNFGNEILRMIDIYREK
                     NNRQIEKVILTGGTANMPGLTEYFTEFLNLPVEKAWPFKQIKYQQFLEPLLRDTAPFL
                     SIATGVAMKGLE"
     gene            30035..30568
                     /locus_tag="PL075_00130"
     CDS             30035..30568
                     /locus_tag="PL075_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOD96652.1"
                     /translation="MADFQAIEPLNKKKTTGWMTNLITVAVIIFVVVFALYFVGVFYE
                     NYITKNISDINDKIKDLAKEIPIEDRNEVLTFYSQLINLRALLANHLYPSNIFSRLEL
                     ITHPQVTFTTFSYENKTSIIKLEGYARDLNVLAQQLLALQKTTDFVKITVNDIKNAAD
                     KVQFGVEINFNPTFIHK"
     gene            30572..31183
                     /locus_tag="PL075_00135"
     CDS             30572..31183
                     /locus_tag="PL075_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96653.1"
                     /translation="MKESEKRTISIIIILALFIGAAIVFFTMTWPMFDQVLKDNRNLA
                     LAKAEYQRQVEAVRLAKTIVEQYKNLTDVNQMISLTMPRSEELYNAIVQLNKISEDSG
                     LSIQSIALQKIASATTAPSKTLSTSSQSLLKSPQQLALTLSLNGTYESFKTWLEAVET
                     NIRLMDITNISFSGVKATPTERGGDAGFNFFNFSVALNLYYQP"
     gene            31189..31560
                     /locus_tag="PL075_00140"
     CDS             31189..31560
                     /locus_tag="PL075_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96654.1"
                     /translation="METIEQKSKTNIFIYIIIAILIVAIVGVYFVFFAPEKLATSETP
                     TAIPVTNTTVEEVGSSPATKTTSAPLVSHSTSTDITGGLSSLKLTPEDILNNPIFQSL
                     RAYAEPVEVPTLGRPNPFIPY"
     gene            31577..33337
                     /locus_tag="PL075_00145"
     CDS             31577..33337
                     /locus_tag="PL075_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOD96655.1"
                     /translation="MIPNKILLELLVKNNKIDQATADKLLKDSQTLNQPVEEIIYERN
                     LVPQDEVAKIKSEYYKIPTKHFTKEDIIPNEVLNIIPEEVARTHQIVAFKKDGDTLHV
                     GMLSPDDQQAQDILKFIAKQRKLNIAIYLVTKSDLENIWKNYRSFATRLKEIQDSVDI
                     FKRQSKPSHQLYQQRVVRIDEASGIIAEEAPIITIVSTVLEEAVESNASDIHIEPMRN
                     KLRIRFRLDGELKEVLSLPLELQPAIIARIKILSDLKIDETRIPQDGRFRTILDNKEI
                     DFRVATFPTALGEKVAVRVLNPYVGLKTIDDLGVRAYHKTLIEEAIAKPFGMILATGP
                     TGSGKTTTLYAILQGLNKEAVNIVSLEDPVEYFIEGLNQSQVKPEIGYDFASGLRQIV
                     RQDPDIIMVGEIRDNETAALAVHAALTGHLVLSTLHTNNAIGVIPRLIDMKIEPYLLP
                     ASLILMMAQRLVGRLCDQCKQKVEATGSLKEEIEKSIAALPKELRAKYQKDKYEIYLP
                     QGCKECNYKGIKGRMPLFEVLKMTPSLEEIVLKDPSAANLQQEAEKQGMVTLRQDGII
                     KALEGLISIEEVLKETMEKD"
     gene            33365..34441
                     /locus_tag="PL075_00150"
     CDS             33365..34441
                     /locus_tag="PL075_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOD96656.1"
                     /translation="MADVFYKEKIENLLTLTAQQNASDLHFSVGRKPTLRIDGRLVSI
                     AKEEVFTKEVVEGLIDALVPSGRKEEFLKNREIDFSYTLSDKARFRVNVYFQRGNMAA
                     ALRFIPYKIRTIEELNLPPILHDFAKYSQGFILAVGPAGHGKSTTLAAIIDEINHERT
                     AHIITIEDPIEYVFVQDRSIIDQREVLSDTLSFHRGLRSVLRQDPDVIMVGEMRDPET
                     IQTAITAAETGHLVFSTLHTNSASQTIDRIIDSFPASQQSQIRTQLAATLVGIISQRL
                     VPRLNGGRIPACEVMLTNSAIRNLIREDKIYQIDLVIETSIQERMISLNRSLANLVKQ
                     SEISLETAESYSLNPAELANLLAR"
     gene            34451..35662
                     /locus_tag="PL075_00155"
     CDS             34451..35662
                     /locus_tag="PL075_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD96657.1"
                     /translation="MAKFRYTGRNDLGEIQSGVVEAGSRDLAIKILQDNKIYVLSLES
                     LEKKGFLDRLLQSFQRVKTKDLMIFTRQLSVLLSAKVQLVDSLKALYNQVTDPLLKDA
                     IFDVFTDVEGGMYFSQALAKHPKVFSDFFVNMVRSAELSGRLEETLNYLADYLEKDNN
                     LKTKLRNAMIYPAFIIVVFLAVMILVVTVVIPQLKGIFTESGSQLPFITRVLMSVGDF
                     LLNWGVAIIVFLVIAIFLFVRYIRTPEGKAFWQQLLLSLPIIGPIQKKVAISRFASSC
                     SALLKGGLPIASSLEISGKIVSNIVYEEILTKTADAIRSGGTISNFLAQYPDYFSPMV
                     IQMIAVGEVSGQVDELMQKVADFYSNEVDQTAANLAEIIQPVLIVILGVFIGVFVAAV
                     LMPIYNLAQVF"
     gene            35727..36185
                     /locus_tag="PL075_00160"
     CDS             35727..36185
                     /locus_tag="PL075_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD96658.1"
                     /translation="MNKKEKGFTLIEMLVVVAVIGLLASLILVGLSSFRTRGRDTRRV
                     ADTKEIQNGLELYYMKNGSYPVLTSGNWDDLEQELVNANLGITNLPQDPTAPTRSYDY
                     GSDGQHYVIAVTIEDPDANKSLLDSDLDGSQYGGVNCGDASNDTIYCIGM"
     gene            36197..37117
                     /locus_tag="PL075_00165"
     CDS             36197..37117
                     /locus_tag="PL075_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOD96659.1"
                     /translation="MLQPVIIAIVFLLGLGVGSFINVITIRFQPDKNIPFIKLVSGRS
                     RCNFCKHQLKWYDLIPIFSFIFLKGKCRYCGKKLSWQYPIIEILTGIIFAGVTYRLLR
                     LDVIRLLLASGTIPFWIWLVFLLFLFYSAVLITLSIIDIKHYLLPDKLIYSAIGVSVV
                     ADLLFYGLSKSSKFNFPSCGLNFLSSYMDYFNCQFNVLTSYLFGALAVGGFLFLVYLF
                     TRGKGMGLGDVKLGILIGLMLGLTNGILALVMAFIIGSIVGIILLASKKKSLKDWVPF
                     GPFLALGVFIVIFWGSFFVESYFSIFNYLI"
     gene            37114..37581
                     /locus_tag="PL075_00170"
     CDS             37114..37581
                     /locus_tag="PL075_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96660.1"
                     /translation="MKEVPISNTLYWAAIIFMAIVFVATIILNSYLFVLDNQLTQQIY
                     LSRVALQKATLIIDYGNGQRKIFEGDVPAGGLSLYDVLLASAQTGSISVQFSKTADGR
                     VSLVKIDQYVNNMDHYWELQIPKFNWKRALNDSTIDLTQFYLVGGNTAYLVYK"
     gene            37595..38422
                     /locus_tag="PL075_00175"
     CDS             37595..38422
                     /locus_tag="PL075_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOD96661.1"
                     /translation="MEIINGENISQEILEDLKPFFQKHKAALAVISIGNNLGGEVFIK
                     QKKKVADYLSIEFIHFHFKEDISNKLLSKKINQIVRQNFIKGVVLQLPLPKKFNTYAL
                     VNIIPSQKDVDVLNNAHFASYCFQRSLILPPAVETIKIIFEKYNINVQEKNCGIVGVG
                     RLVGFPTLIWLLQQKATVFAVDISTKQPEKILAQCDIIITGTGSPHLINKKFVKKGAI
                     IIDFGFSKENGKIKGDVNIDSVKKKALLVTPTPGGTGPILVAALYKNLKTLLQSKQK"
     gene            38470..39171
                     /locus_tag="PL075_00180"
     CDS             38470..39171
                     /locus_tag="PL075_00180"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOD96662.1"
                     /translation="MELAANPGKKIIIEVDGIKWARYPIKTHFITEKDNLDEIIKKYV
                     LPNAQKGDIICLGQKIVSILQGLIIYKKDLKVGWWANFLSKFVKKTPYGFSVGNPLKM
                     QIAINLAGLPRILFAAVVAGISKIFGFSGVFYRLAGHQISQIDGFYAEAFPQYGEMGI
                     LGPKNCNELCENLQKKYDHSFLVIDINDLGGNILGCSKDLAGKEGLILKILKDNPAGQ
                     SNQQTPIIILRQLCN"
     gene            39162..40646
                     /gene="murJ"
                     /locus_tag="PL075_00185"
     CDS             39162..40646
                     /gene="murJ"
                     /locus_tag="PL075_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOD96663.1"
                     /translation="MQLKNIINHETKSVNSAAIILAIASLVSALLGLIRDRLLAGTFG
                     AGRELDIYYIAFKIPDFISIVLIIGAISAAITPIFSHYLVRSKKEAFDFLSNLINVFL
                     LFLIILSLILIIFVPQLLYLIAPGFSVEEREMAAVLTRIMFLSPILLGISNIISSVLR
                     VFQRFLVTSIAPILYNVGIIIGILFFVPIAGLKGLAWGVVLGAFFHLVFQIPTLIQLG
                     FKFQKKLKLRDPGVKKAIELTIPRSIGLATTQINLIIISAIASILSSGSIAIMNLADN
                     LSRPLYTFIAVSLATAAFPALTLAFSKQEKNKFQDIFSATFKKIFWLTFSLGIILFLF
                     RDLIVKIILHTGKFALTDVRLTAICFGIFAVGIFAQGLVLLIAKSFYALHDTKTPTII
                     SILSAIFNIVLCFGFIKLLSFPNAFSQFFLNLLNINSLQLTSVAAHPFIVKFLGIYNL
                     TGIEVIALPLAISVSAVFQFIFLYILFCRKKEKVFQEQSLSEIN"
     gene            40646..42520
                     /locus_tag="PL075_00190"
     CDS             40646..42520
                     /locus_tag="PL075_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOD96664.1"
                     /translation="MKNIRNFAIIAHIDHGKSTLADRFLELTGAIDKRKMKDQFLDRM
                     DLERERGITIKMQPVRMIYRSRTNSEYILNLIDTPGHVDFSYEVSRSLAAVEGAILLV
                     DATQGIQAQTMGNYQLAKKQELTIIPVINKIDLPNAQIEEVEKQIMDLCGISQKEIYK
                     VSAKTGEGVEELLQAVIEKIPSPTESDKKPLRALVFDSIYDNHRGVISYVRIVDGEVR
                     GGETGFLMASETLFKVAEVGIFRPWLEKTEILSSGEIGYIVTGLKEPVKVRVGDTITK
                     FKKSSPSYSSANSKNKNDKIIKETGKNKYLSIAGVERLPGYKEPEPMVFASVYPTSNE
                     NITTLKNAIQKLKLNDPSLVYKTEFFSALGQGFCLGFLGLLHLEIVKERLEREYKVDV
                     IFTTPSVNYKVVMKNGLVKDVFRASDFPVRDQIEKILEPWVRLEIITPLNFVGAVMQL
                     KDRHRLNYLTTQYLGTLIILVFEAPLSEIIINFYDKLKSATEGYASMSYEFLEYREGD
                     LVKLEILLAGDKEESLAKIVHRRFLEEEARKMVEKLRSVLPRENFAVAIQAVAENRII
                     ARETLPALKKDVTGYLYGGDRTRKMKLWKKQKEGKKRLKERGKIRLSTKAYLDILKME
                     "
     gene            42650..43033
                     /locus_tag="PL075_00195"
     CDS             42650..43033
                     /locus_tag="PL075_00195"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOD96665.1"
                     /translation="MAWRSKILLVVLFIVASFTLIYFYHQLTLEKKLQADYEAIVQRV
                     NELQKENALLEDELAKMENEEELERLARELYALKKPGEKAMIIPQEVMEKLIEDDAIQ
                     NVSPQAPSKISQWFQEIKHFFGNLF"
     gene            43064..43134
                     /locus_tag="PL075_00200"
     tRNA            43064..43134
                     /locus_tag="PL075_00200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43096..43098,aa:Gly,seq:ccc)
     gene            43170..43242
                     /locus_tag="PL075_00205"
     tRNA            43170..43242
                     /locus_tag="PL075_00205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43203..43205,aa:Thr,seq:cgt)
     gene            43274..44410
                     /gene="prfB"
                     /locus_tag="PL075_00210"
     CDS             43274..44410
                     /gene="prfB"
                     /locus_tag="PL075_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOD96666.1"
                     /translation="MNDLKSKIQEIRNNIKKLEKEIDLEKKKLELEELNKKMSRPDFW
                     KVPEETVDILKRVKELKGEINFWMELEKKLDEVEDLSESIDSDKSSADLENQLINLEK
                     QIEDKKLEIFLSDPYDKNEAIMILTAGQGGRDAEDFCRMLFRMYSNYFAKKKWPYQIL
                     YQHFSEEPGGSKDAGGEMGLKNISLEINTPYAYGFLKNESGVHRLVRISPFDAKKQRH
                     TSFVLVEVLPQIEEIDLKDIELRDEDLKIEFMRSSGPGGQNVNKRETAVRIVHLPTGL
                     MAVCQSERSQQQNREKALHLLKLKIFNALQQQKETERKNLRKRIEPSWGNQIRNYVLQ
                     PYKLVKDLRTNVETSNIDAVLDGEIDNFIKAEMLLNLNPSSKRK"
     gene            44554..45234
                     /gene="ftsE"
                     /locus_tag="PL075_00215"
     CDS             44554..45234
                     /gene="ftsE"
                     /locus_tag="PL075_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOD96667.1"
                     /translation="MVYFQNVTKVYNSHTVALDNINIKIMREEFVAIVGKSGAGKSTL
                     LKLLIGEEKPTKGRIFFEKQDVSRLSYKELPQLRRQIGMIFQDFKLLPDKTAFENVAF
                     ALEVAGYRPKEIKETVPQMLELVGLKNKIFNFPHELSGGEKQRVAIARALINKPDLLI
                     ADEPTGNLDPINTAEIIKLLLKINELKTTVILATHNKNVVDMLNTRVISLDNGRVIRD
                     EEEGQFII"
     gene            45241..46149
                     /locus_tag="PL075_00220"
     CDS             45241..46149
                     /locus_tag="PL075_00220"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOD96668.1"
                     /translation="MFVFLARIIKYGFQSFWRNGLLSSATVAIMTLTLLLCSGLIIFG
                     NAGNVVLQSLESKIDVSVYFKTNVGENQILAIKKNIEQLDEVASVEYISQEDALATFK
                     ERHKDDPVIIQSLEELGYNPLSASLNIRAKNPRNYEGIANYLEKEIPSEFIDKINYSQ
                     NQIIIDRLNKIISAFQTGGLIAVGVLALVAFLITFNTIRLAIYSNRESIGIMRLVGAS
                     GSLIRGPYVVEAFIIALASTILSSILLIPILYSIAPYFNNFIPEFNIIEYFWSHFFYL
                     FGIQFLVGLALAILSSVIAIRKYLKV"
     gene            46307..46377
                     /locus_tag="PL075_00225"
     tRNA            46307..46377
                     /locus_tag="PL075_00225"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46339..46341,aa:Gln,seq:ctg)
     gene            46521..47102
                     /locus_tag="PL075_00230"
     CDS             46521..47102
                     /locus_tag="PL075_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOD96669.1"
                     /translation="MSKTVKILILSDFIIISAFGFLNPIFALFLTDKIAGGTIETVGY
                     ATAFYWVAAVLVRLPLARRVDSTKSEKDDFYFMILGSILISLVPFLYIFSTQIWHIYL
                     IQALYGLGYSLRLPGWYAMFSRHMRKGREGYEWSVNSMVAGLGSAITAALGGVLVGRL
                     GFDVLFLFIGLLSVFGSIVLVFLYYILEPNHHV"
     gene            47333..48091
                     /locus_tag="PL075_00235"
     CDS             47333..48091
                     /locus_tag="PL075_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014127167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HOD96670.1"
                     /translation="MKDFLQKITIDGRAMYLAYDHGFEHGPIDLPNQSINPDYILNIA
                     VEGGYNAIILQRGLAEKYYTGTKYQKQIPLILKINGKANIWQGGDVYSYQNCSVKYAK
                     TLGASAVGYTIYLGSAHDGKMFSDFGKIVEEAHQLNMAAIAWVYPRGEFVKDEVSKEI
                     TAYAARIGLELGADMVKIKYSGSKENFAYAVKAAGKTKVVLSGGPKVNDEEFLQITKD
                     VMAAGASGVAVGRNVWQHSEPLKITKKIKEIIFS"
     gene            48226..49425
                     /gene="tuf"
                     /locus_tag="PL075_00240"
     CDS             48226..49425
                     /gene="tuf"
                     /locus_tag="PL075_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOD96671.1"
                     /translation="MAEKKKFERKKPHVNVGTIGHIDHGKTTLTAAILHVLSFKGLVE
                     KVEGVDQIDNAPEEKARGITIALHHSEYESEKRHYAHIDAPGHADYIKNMITGAAQMD
                     GAILVVSAADGPMPQTREHILLARQVGVPAIIVFLNKIDQVSDPELIDLVENEVRELL
                     TKYQFPGNEVPVIRGSALAALNAKSLDDPACKPILDLIEAMDNYIPQPVREIDKPFLM
                     PIEDIFSIEGRGTVVTGRVERGILKTNEEVEVVGLRPTIKTVVTGIEMFNKTLDEAQA
                     GDNVGILLRGLKKEDVERGQVIAKPGTVTPHTEFEAEAYILTKEEGGRHTPFFTGYKP
                     QFYIRTTDVTGEVTLPQGIEMVMPGDNAHLIVKLIAPVALEEQQRFAIREGGRTVGAG
                     VVSKIIK"
     gene            49446..49769
                     /gene="rpsJ"
                     /locus_tag="PL075_00245"
     CDS             49446..49769
                     /gene="rpsJ"
                     /locus_tag="PL075_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOD96672.1"
                     /translation="MSKSNENQQKIRIKLKAYDHKVLDNSCKQIFETANRYVKDIIGP
                     IPLPTEIKRYTVNRSTFIDKNSREQFEIRIHKRLIDILNPNQKLIDALMNLNLPAGVD
                     IEIKM"
     gene            49901..50512
                     /gene="rplC"
                     /locus_tag="PL075_00250"
     CDS             49901..50512
                     /gene="rplC"
                     /locus_tag="PL075_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOD96673.1"
                     /translation="MTKFILAQKIGMTQIYQDDKAIPVTALEAGPVVVSQIKKQDRDG
                     YYAIQVGFGKKRNLSKAVRNHLKGLGDLRWLKECKITKEEADAFKRGDRIDVSVFKEG
                     EKVKIIGTSKAKGFQGVVKRHGFHGGPKSHGQKDRLRAPGSIGATHPQHVLKGKKMAG
                     RMGGNRITLKNVKIVKIIPEKNIICVKGAIPGKRGELVQILSE"
     gene            50516..51148
                     /gene="rplD"
                     /locus_tag="PL075_00255"
     CDS             50516..51148
                     /gene="rplD"
                     /locus_tag="PL075_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOD96674.1"
                     /translation="MKTDLYNQKGEVIGQIALSDKIFGAKWNPVLVHQVLVAIKSNQR
                     KPIAHAKDRGEVSGGGRKPWRQKGTGRARHGSIRSPLWIGGGVTFGPTKEKNYQKKIN
                     KKAKQQAFISALSKKMTDGEIKVVETIHLEHSKTKEMAKILSSFLNTKKRNQGLIVLP
                     SSDPSIIRATRNLSFAAATIADNLNLPDILKYKNIIFTKEAIEKLNEKIK"
     gene            51153..51578
                     /gene="rplW"
                     /locus_tag="PL075_00260"
     CDS             51153..51578
                     /gene="rplW"
                     /locus_tag="PL075_00260"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOD96675.1"
                     /translation="MSIFSKKQSKKEESEDQDTLKEEKKLKEEKKETEKPSLKKEGRG
                     VIFNKNILLIKEPKVTEKSQSIQAYRQYVFNVVRQANKNEVKKAIERIYNVTVKKVRM
                     INIPAKKRRSGRSVGKKSGYKKAIVTLKEGQQIEIMSQK"
     gene            51583..52407
                     /gene="rplB"
                     /locus_tag="PL075_00265"
     CDS             51583..52407
                     /gene="rplB"
                     /locus_tag="PL075_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014095242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOD96676.1"
                     /translation="MKKHKPTTPSRRHMTAVEYKEIVTTSEPLKKLTAPLKQQAGRNS
                     FGRITVRHRGGGNKRLYRLVDFKQDKFNIKGTIKEIEYDPNRNTYIGRVAYEDGEYRY
                     VLLPEGMKTGDQIITTELTSITPGNRLPLKNIPVGTRVFNIELLPKSGGKIARSAGSA
                     AEVMGIEDKYCILKMPSGEIRRVLANCYATIGLPSNSEYRTINIGKAGRTRWLSVRPT
                     VRGTAMNPCDHPYGGGEGRQPRGTKRPKSAWGKVTGGHKTRNKKKWSNSLIIKRRK"
     gene            52409..52714
                     /gene="rpsS"
                     /locus_tag="PL075_00270"
     CDS             52409..52714
                     /gene="rpsS"
                     /locus_tag="PL075_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOD96677.1"
                     /translation="MSRSLKKGPFINPKLLKKMAKIKPDSGQVIKTWSRSSTISPEMI
                     GYTFAVHNGKDFIEVKVTEEMVGHRLGEFAPTRKFIKHGGKMQRELEKKAVVNEPAK"
     gene            52724..53254
                     /gene="rplV"
                     /locus_tag="PL075_00275"
     CDS             52724..53254
                     /gene="rplV"
                     /locus_tag="PL075_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOD96678.1"
                     /translation="MAKENVAIAKLKYLHMAPRKVRLVANSIKGKHVIEAEAQLVYAP
                     QRAAKPMLKLLRSAVNNASNKKLDKNKLFVASITVDEGPMLKRWLPRAMGRATPIQKK
                     MSHITLVLEEKGEFNRYKVPQAKITKSEKKLTETKGGVKEKEHELKKKSPTVEETKKK
                     PQEGFARKIFRRKEIA"
     gene            53269..53985
                     /gene="rpsC"
                     /locus_tag="PL075_00280"
     CDS             53269..53985
                     /gene="rpsC"
                     /locus_tag="PL075_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOD96679.1"
                     /translation="MGQKIHPKSFRLGINKNWSERWLASKKTRPIILEEDVKIRNFLN
                     KKLRNALIADVEIERLENNINLIIKTARPGVIIGRGGKGIEDLKKEVEKIIRRHRKER
                     KYPLNYVLNISIEEVKNPTANAAIVAQSIAASIEKRMPYRRLMKKAIEDAKQNKEVKG
                     IKIKLSGRLNGVEIARSEWLDWGAMPLQTIRADIDYSEATAFCTYGTIGIKVWIYKGE
                     IFDKNKNKEGISEMSNKQTK"
     gene            53988..54401
                     /gene="rplP"
                     /locus_tag="PL075_00285"
     CDS             53988..54401
                     /gene="rplP"
                     /locus_tag="PL075_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOD96680.1"
                     /translation="MLMPKKLKHRKYQKGRSRKRSIETRGTFLTFGSYGLQALDNIWL
                     TAQQIEAGRKAIVGYLKKGGKVWLRVFPDKPITKKPPEVTMGGGKGDLDHFAFPVRKG
                     RIIYEIDGVSEEIATEALRRAGTKMPIKTRIVKKE"
     gene            54414..54623
                     /gene="rpmC"
                     /locus_tag="PL075_00290"
     CDS             54414..54623
                     /gene="rpmC"
                     /locus_tag="PL075_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOD96681.1"
                     /translation="MEFKELKNLPKSELEKLLAQNRDKIKELRFNLAFGKVKDISEIK
                     KTKKSIAQILTLLNSQKNESKKSSN"
     gene            54598..54897
                     /gene="rpsQ"
                     /locus_tag="PL075_00295"
     CDS             54598..54897
                     /gene="rpsQ"
                     /locus_tag="PL075_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOD96682.1"
                     /translation="MNQKNHPIKKRLEGIVISDKMDKTVVVKVERFKKHPFYHKIMTV
                     SKHFKAHNENNEAKEGDYVIIEETRPLSREKRWRVVQIVKKGNSKNLVGSDKINE"
     gene            54907..55275
                     /gene="rplN"
                     /locus_tag="PL075_00300"
     CDS             54907..55275
                     /gene="rplN"
                     /locus_tag="PL075_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOD96683.1"
                     /translation="MIQPRTMLKVADNSGAKIVQCFKVLGGTRRRYAQIGDIIIASVK
                     MAQPRKSVSKKDIVRCLVVRQKKPYRREDGSYIRFDDNAVVLIDNKNQPRGTRIFGPI
                     PREIKQKGFEEVASLAVEIL"
     gene            55296..55601
                     /gene="rplX"
                     /locus_tag="PL075_00305"
     CDS             55296..55601
                     /gene="rplX"
                     /locus_tag="PL075_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOD96684.1"
                     /translation="MKIKKGDEVVVLVGKDKGKRGKVIVVLPKNNKAIVEGLNLVKKH
                     RRPKRQGEKGEIISLPRPIDVSKLALWCSRCNRQTRVGYRFEDDKKLRICKRCQQLI"
     gene            55583..56146
                     /gene="rplE"
                     /locus_tag="PL075_00310"
     CDS             55583..56146
                     /gene="rplE"
                     /locus_tag="PL075_00310"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOD96685.1"
                     /translation="MSAIDLKTKYQKEVRPKLQKEFRLKNVYAVPSIKKVVINVGIGK
                     MLSTAAQSKNIDDFLKQIKQDVSLIAGQWPKESRAKKSVSSFKIRKGDVVGLTVTLRG
                     QRMYDFLARLVNIALPRTRDFRGISIKNLDEHGNLNIGIAEASIFPEVTNNVLKHPFG
                     LEVTIVVNDKNKERVVNMLKSLGFPIK"
     gene            56148..56333
                     /locus_tag="PL075_00315"
     CDS             56148..56333
                     /locus_tag="PL075_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOD96686.1"
                     /translation="MAKKSVVARAKKPPKYSTRIVRRCFRCGRKRGYLRDFGLCRICV
                     RELSRKGEIPGMKKASW"
     gene            56350..56739
                     /gene="rpsH"
                     /locus_tag="PL075_00320"
     CDS             56350..56739
                     /gene="rpsH"
                     /locus_tag="PL075_00320"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOD96687.1"
                     /translation="MDTISDFLNQIKNALAAKKPFLLTPHSKMKEEIAKILLEEQYIS
                     NYKVSGKAPKKILRIDLKYNEDGTPAIREIKRISKPGCRRFIKTKNIKRVKSGYGRYI
                     ISTPNGLMTNIEAQKHHLGGEIICEIF"
     gene            56744..57286
                     /gene="rplF"
                     /locus_tag="PL075_00325"
     CDS             56744..57286
                     /gene="rplF"
                     /locus_tag="PL075_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOD96688.1"
                     /translation="MSKIGKKLIVIPDNVKIEVLENEVMIKGPKGELKVPLFPDFNVE
                     LEGNYLKIIPTREKKFTSAFWGTLRSLIANAVKGVSEGFEKKLEIEGVGYRANVEGNI
                     LVLNLGFSHPIRFEIPKGIDIVVAKNLITISGADKQLVGQTAAKIRSFRKPEPYKGKG
                     IHYVGEHIRRKAGKKATATA"
     gene            57308..57661
                     /gene="rplR"
                     /locus_tag="PL075_00330"
     CDS             57308..57661
                     /gene="rplR"
                     /locus_tag="PL075_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOD96689.1"
                     /translation="MKQKRTKKEKRIRRHKKIRANISGTKTKPRISVFISNKHIYVQA
                     IDDEKGVTLTSVSDQDLKKKSKKLELSKEVGLTLAEKLKKLNIKSAVFDRGGFQFHGR
                     VKNIADGLKERGIKC"
     gene            57666..58247
                     /gene="rpsE"
                     /locus_tag="PL075_00335"
     CDS             57666..58247
                     /gene="rpsE"
                     /locus_tag="PL075_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOD96690.1"
                     /translation="MIKNNSTINKKKESVVELKNQGYEDKLLDLRRVTRVVAGGKRFS
                     FRATVVVGDRAGKVGVGVAKGKDVSEALAKAKTKAQKRMVQVTIVNDTIPHEVKAKYC
                     AAEVVLKPARKGHGIVAGGAVRAVCELAGIKDLSAKILSRTPNKLSNARATINALQKI
                     KPKRVNQSDTSKSQNQQLAKSEDQASEVLKTEN"
     gene            58250..58663
                     /locus_tag="PL075_00340"
     CDS             58250..58663
                     /locus_tag="PL075_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HOD96691.1"
                     /translation="MQLHELKKEYKTKKAKRVGRGGKRGTYSGRGSKGQKARAGAKIR
                     PAVYDLIIKIPKKRGVKHKSITEKPVLINFDILEKHYQEGDIISPQTLFEKGLIKKRG
                     GVLPKVKILGRGNLTKKLIFKKCQFSQQALAKIKK"
     gene            58669..59946
                     /gene="secY"
                     /locus_tag="PL075_00345"
     CDS             58669..59946
                     /gene="secY"
                     /locus_tag="PL075_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOD96692.1"
                     /translation="MSILSKVALLFKIKDLRKKILTVIFILVIFRLFATLPIPDIDTA
                     RLNAIFASNQLLGILNIFSGGTLQQLSIMMLGVGPYITATIIMQLLTMVIPRLKEIYY
                     EEGEKGRMKFNQWSRYITVPLCMLQAYAFLNLLISQDVLPHLSTLSLIRNVLVVTAGS
                     MLLMWLGELISEYKVGNGISLIIFAGIVSGWPASLRSAILSYTPSMLPTYLMFLVVAI
                     IVILGVVYINEAERKIPIAYAKRVRGNKMYGGVSSYLPLKVNQAGVIPIIFAISILLF
                     PQFIGQILVAIKTSWAASAGNFLMNMLNNQLVYSILYFLLVVVFTYFYTMVTFDPKEI
                     STNLQRNGGFIPGIRPGQTTADYISFVLHRITFVGALFLGVIAILPNILQGVTGTQFL
                     TIGGTSILIVVSVAIETIKQVESQLVMREYEGF"
     gene            59965..60657
                     /locus_tag="PL075_00350"
     CDS             59965..60657
                     /locus_tag="PL075_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOD96693.1"
                     /translation="MKKAKILIFYGPPGAGKDTQARRIAFKSGAVIVSSSKLIEERIF
                     DPQLQNDPIIQRERKNYESGALCTPEWVTQIINDKVKELAAQKQDLIFTGSPRTIYEA
                     ENEIPYWETFYGQENIHPILINLKPETSVFRNTHRRVCEKCGYPVIYSWETEMWKFCP
                     LCGGKIIDRGVLDDEATIKVRLEEYENRTKPILAYFQKRGYQVIEVDGEPLPDIVTQN
                     LIEALAKINFFD"
     gene            60659..61417
                     /gene="map"
                     /locus_tag="PL075_00355"
     CDS             60659..61417
                     /gene="map"
                     /locus_tag="PL075_00355"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOD96694.1"
                     /translation="MIKLKSDEEIQKLKEGGRLLSKIIYEVAAKAKAGISTRELDWLA
                     ENSILKCGGEPAFKNYQPDFSLKPFPATLCVSVNNVIVHGIPGNYLLRNGDLVKLDLG
                     MKYKGFFTDMAITVGVGKISSLAQKLIRVTRKALDLAIQAAQVGATLGDIGYAIEQYV
                     RSEGFYEIRVLSGHGVGYAPHEDPDILNFGVPHKGIKLKEGMVIAIEPMVAVDSSDVI
                     ELADGSFVTKTGCLAAHFEHTIAITSRGPIILTK"
     gene            61419..61844
                     /gene="ruvX"
                     /locus_tag="PL075_00360"
     CDS             61419..61844
                     /gene="ruvX"
                     /locus_tag="PL075_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOD96695.1"
                     /translation="MANVLAIDYGTKRIGLAIADEKLKIPLPLKPIIEKNQQQVLEKI
                     ANLCKEKNVDQIVIGLPLSFEFEETSMCQEIKAFGENLKQITGIKIIYENEVLTTEAA
                     KKMRQTTSQLKSRKDKSKNHFDSQAASLILESYLRKVKS"
     gene            61877..62326
                     /locus_tag="PL075_00365"
     CDS             61877..62326
                     /locus_tag="PL075_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96696.1"
                     /translation="MKRNSFYIIVIIILVILLAFVIYAYLSDYQKAKENLADRIISAH
                     LEGFPAGTSVKEGMQGEEKTVFRKGEWMGVSGKIKTNEGGDLFLKIYDLAGNLIQENE
                     KWHKSLQAGQGDFGMCCIVVPNEVGQYEARLILNEEVLYSLNFSVTE"
     gene            62368..62619
                     /locus_tag="PL075_00370"
     CDS             62368..62619
                     /locus_tag="PL075_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96697.1"
                     /translation="MKKYFISFVEEFIFNPKYQDHIAGRVEIYLRKSQYDIDELRFFT
                     KKIKKFNRFRNKWDLEEVSESQLEKIRKIIAKEFNEIEE"
     gene            62779..63510
                     /locus_tag="PL075_00375"
     CDS             62779..63510
                     /locus_tag="PL075_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD96698.1"
                     /translation="MAKPFLSVIIPAYNEAKRLPKTLLDMDKYLTQAGFSYEIIVVND
                     GSKDHTAEIVTKMREFIKNLYLIDNKDNQGKGGVVAQGMLAAKGTLRLFSDADNSTSL
                     DQVEKFFPYFKQGYDVVIGSRYVKDSELPVKQPFFRQLPGRIGNFLIRLLAVPGIKDT
                     QCGFKAFTEEAASKIFPQLTIKRWGFDIEILVLARLMGFRIKEVGVRWINDPDSKVKA
                     SAYIEVLTELIKIRLNIWRKKYKIN"
     gene            63511..64560
                     /locus_tag="PL075_00380"
     CDS             63511..64560
                     /locus_tag="PL075_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOD96699.1"
                     /translation="MKNLRKNSKKRKLNNHLVYISELRRDPVSNDWVTIASVRAKRPE
                     QLIIKKPQRIVTPKSQCPFENPAVSGHKPPELIKSLPHKKDWFLQIFENKYPAFQPKN
                     ISISERKYGPYTIINGYGYHELLVLRDHFKPIADYSTSELAFILQALQERYRQIAQDK
                     KIKYISIFHNWGSSAGASIYHPHFQIIAIPVIPPRINHSLIDSKHFWKKHRQCVYCSM
                     NKFEIAQQKRILFQNKKAMALSPFVSVEPFALRIIPLNHIPYFEDTPSSALNEVAEVL
                     RKSLQVLKKKLDDPDYNFFIHTAPTFKKNSYQYYHWHIEIVPKISISAGFELSTGIEI
                     TAIDPDEGIRYLKSK"
     gene            64563..65207
                     /locus_tag="PL075_00385"
     CDS             64563..65207
                     /locus_tag="PL075_00385"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOD96700.1"
                     /translation="MIEVIFSAIANFILNLISQMGYAGVFILMALESCNIPIPSEIIM
                     PFSGFLVSQGRFNLYWLALIGSLGNLIGSLVSYGIAFKLGNKVKEFFLKSKFFATDYL
                     KAENFFKKYGVRSALIARVLPVIRTFISFPAGIFKVSLGKFSVYTFFGSLIWSYVLAY
                     IGLYFGEQWQIIENYFHRFDYIILIVIVGFVCYYIYRKVKQINSRNSHNNSIEN"
     gene            65209..66180
                     /locus_tag="PL075_00390"
     CDS             65209..66180
                     /locus_tag="PL075_00390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOD96701.1"
                     /translation="MTKKYKVVVTRRIPEAGLRLLKKAGFEVKVSPYDRILTRQELKK
                     FVRGADAILSLLTDKIDGEILDAAGSQLKIVANYAVGYDNFNIDDFKARKVYATNTPG
                     VLTETVAEHTIGLLFAIAQRIVEADEFVRQNKFRGWEPMLFLGTDIRNKTLGIVGLGR
                     IGGEVAKRMRDGFGLKIIYFDAMRNKSLEKQLGIQYTPFKSLLKQSDFISIHVPLTLK
                     TKHMFTLKEFRMMKPTAYIINTSRGAVIKEEDLAKALKAKIIAGAALDVYEFEPKITP
                     DLLKLPNTVLLPHIASASIETRSKMAEMAAKNIISVLRGKKPINPIF"
     gene            66275..66961
                     /locus_tag="PL075_00395"
     CDS             66275..66961
                     /locus_tag="PL075_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96702.1"
                     /translation="MVVQSWADILVASFQTWWSAFVNFLPNFLGALIVFLIGLLIAAG
                     LGSLVNKIITAIKLDKLLSKLGVDKFCERAGWKLNSGKFLGEVVRWFFILVFLLAATD
                     ILKLTAISGFLKDVVLYLPNIVIAIIIIIASIIVADFLASLVRGTVKGANLYASQFLS
                     SLTQWVVLIFGFLTALLQLGVGVTIINTVITGVIAMFAIAGGIAFGLGGKDYAAYLVN
                     EFRQRIEEKK"
     gene            67321..>69983
                     /locus_tag="PL075_00400"
     rRNA            67321..>69983
                     /locus_tag="PL075_00400"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT        23635 a        10080 c        14143 g        22125 t
ORIGIN      
        1 taattattat tattttggtg gttgtttttt acaaaccagg aagtcaaacc acattagcgc
       61 caacggcgac accgggaatt ggaaacacca gtccaactaa gggcgaagta cctaaagacg
      121 cgaaagtata tgaggtaggt gaaacagcgc cagaaggggt ggcagtaccg ctcagcgtct
      181 cggattatgc taccggtaaa ttgagatatt atgaaactaa aatcgagaat gacgttttta
      241 ctcccagcac tttttcttgt tatgaaggcg atttattaaa aatagcaatt actgctgtgg
      301 ataaagatgc tgatattgtg atacccgata tgaatatggc ctttccttta attaaaaaag
      361 gagagacaaa gacattagag ggtcagatgc ttaatccagg gaaatatact ttttattgcg
      421 agaagtgtgg aggtttgaac tcaacggctg tggggtatat tattgtagtg ccaaagagta
      481 gtgaagaata aagaaaaatt tcccagtgtc attttaagca ttttttgtca ttctgagcgg
      541 cttattcttg tcattctgag tattttattt tgtcattctg agtgaaacga agaatcccac
      601 ggagtgctaa aagccgagag atccttcacc cgcctgttcg cctgcctgcc gcctgcctgt
      661 cggtaggcag ggtaggcagg gggggcaggc tccgttcagg atgacaaaac aaaacgtcat
      721 tctgaacgaa gcctgcctaa ccggcaggca agtgaagaat cccatccaca gcagcgattg
      781 ctgattagtg ctaaaatcca tgagatcatt cgggcgttgc cctcaggatg acagtaaaaa
      841 ataggatgat agtaaaaaat aggataacaa tggaaaaaat tttgagactt gcaatttatt
      901 ttatccattc gtaattcata gattagaaat tatgaaaagg caaaaacgaa accaaaggaa
      961 atcatcggaa atagaaaaac taaaaaaaga attatctttg tataaaaaat tagctttgaa
     1021 agatccctta accgggcttt ataatcgccg tggattttta gaggaaacag atcggttgat
     1081 taaaatgaat cagcgattag gtcagcgttt ttctttttct cttgcttctt ttggaagagt
     1141 tggattagta tttattgatt tagataattt taagcacatc aacgatcgtt ttggccataa
     1201 agttggagat gaagttttga aggaaatagc aaaacgttta gaaggttcat tgcgtcgatt
     1261 tgatattgtc gggcggtggg gaggagacga attcgttgtc gcgatttggg atgtggataa
     1321 aaagacattg gatattattg tggaaaggtt aaagagcaaa attactaatg agccgattat
     1381 tattcgaaag cagccgattt ctattacagc tagttttggg gcgaaagttt ttcccattga
     1441 aggactggaa ttggagaagg agattgaggg ggttgataag tcaatgtatc gtgccaaaaa
     1501 acaaagtaaa aatagattaa cctctgccta aacatctgat ttttcttatt atgctatagc
     1561 ataacacgga aaaaattcct ttaaataatc gctgttatgc tatagcataa cagcgattat
     1621 tgtttagctc gttggaaagg agaagtaata aattttattt aaaaggaaga aatgaagaaa
     1681 taattattct tttattatgt taaaagccat tatgtcaaag atgaaatgca gccaacttta
     1741 ttcaagagaa caaataatta atcttctatc gtgctatttt atgcacgaga acatagcatt
     1801 tagatttaaa agatgttatg ctatagcata acaaaaattt gcgttataaa ggaaaatatg
     1861 aataaaaaat tatccaccaa taaaataatt tctctaatcc cttccccgtc cgatttttct
     1921 tctaatcaag aatgggagaa tgcctgctgg gaaaaaattt ctaatcagcc agaagtgttt
     1981 aattttttac tgactccagc tgaacgtaga aaaattatta aaagattagc agttattaag
     2041 gaattacaag cgggtaaaag ctatcgaatg atcggagtag aattaaatct ttctttgcaa
     2101 acaatccaca atattaaaaa aattatgaac gatgagggaa tctattacag ctatttttcg
     2161 cgtgtcagaa aaataaagca aaaagaaaaa gagcaattgt taaatactcg aaaaataaaa
     2221 atagccagac cagaagtagg gaggcgatat cgacgaacga aatttggtaa agttaggctt
     2281 gtataattat agagcttgct atttagagag tttgtgattt gtagagataa tgatggtaaa
     2341 aagaatttta tggggctgtt atgctttagc ataacagccc catctccaac taaatttgtt
     2401 tgatttataa tttgctatat ttgactgtta atttgtaatt tgctaattaa tacagcacgg
     2461 tagtaaaact aagaactaaa taattttagt tggtgttatg ctatagcaca acaccaacca
     2521 ctaccgtaat ttatcaaaaa acagtaaaat tatttaatta ttatttgttt atgcttattt
     2581 ggtaggttgt tttggggttg ttggtcgctt agtctggcgg gagaagaact atgttggttc
     2641 tgccgaggct cgctgttatg ctatagcata acagcgagcc tcggcctcgg cagagaaaac
     2701 agaaggaagc gagagtaaac aactcaactt gagtgacatc ggatttacta taaggattta
     2761 atttgctatt tgggtaacaa tgaaaaaaga agaaaagggc tatgaacaag atgaaaaaat
     2821 gagacaaaga taaaaagaga agaaaataaa ttatagataa gatataaaaa aacaaaaaac
     2881 gggaatctaa gacaaaaagc aacttaataa aaaaccatct tgattaagcg acagctttca
     2941 aatttggaaa ataacctcta ttagattttc aacgaaagaa gtgctataat tggattatgc
     3001 ataaaaatat tagacggcga atttttattt tggctagttg catttttgtt ttggcggctc
     3061 tacttttagt tctttattct tttggttatc gttgggacaa agaaaaattt cagcctgttt
     3121 taacaggagg actatatttg agggttaata ccgttaagcc acaaatttat ttagatggga
     3181 aatttctttc caaagccacg ccgggattat taagcagtgg catttttttg cctaatttat
     3241 tgcctgatac atatcagcta gaaattaaaa aagataatta ttttgagtgg gaaaagaaag
     3301 tggatatcaa atcgcagcaa gtaacgagct tttcttccat tgtgcttttg cctaaaactc
     3361 cactggtaac tgatatttat aaaataaccg tagataatgc cactaatacc aaaacaaaat
     3421 ttcaagagga gattattgat tttttgcctt tggccaatgg aaaagaaatt attattcaaa
     3481 caaaagaagt tagcacaagt actaatgctg ttttaaaaat ttacaatttt attgatggtt
     3541 ccagtgtgga aatttataga cagaaattag caaaaaacga aacactcaat ttaattcccc
     3601 gtttaaaaat caacgacgat gaagctaatg aagtgatttt tgatctaacc aaaaataata
     3661 tccaaatctt ttatttatgg cagaggattg agccagagag aatccagagt ttatcacaac
     3721 aaattacaac gacatttaaa ctgaaaaatc cgattcagaa aattgagttc tatccaaaca
     3781 ttgagggcaa atacattgtt ttaagcggta aggatttgat ggttgttgat tttaccaaga
     3841 atgaaacaaa aaaaattatt agtgatatag ttgattttca atctaaagac tatgtgcttt
     3901 tttggcttga gcaaagcgga gcaatattta gctataattt gattcttggc ttttctactc
     3961 ctttgggtat tattgaatta ccggataaat ttagcgtgac gagaatggaa attgcgccta
     4021 atagtcaatg ctttgctctt ttattagata ataatgagtg gtattttttt agagccggcc
     4081 aatcaatgga aaaaataaat gatgttgtaa aatttgtttt tgcacctgat agtaaaaaag
     4141 ttgtttattt aaccaagacg ggagatgtac gaatgagatt tttaagcgat attgcgggcg
     4201 atgtaataag aaagaagggg gatgaagttt tgattagagc taatgtttct tcaagcgctg
     4261 atttggaatg gtatgccgat agtcagcatt tgattgtagc agaggggaaa ataatttatt
     4321 tttccgagat agacgatcga gatcaaatta atacttttat tcaagagttt gagtttgatc
     4381 attatttatt aacaaataag aagagcggta cggtgtgggg tatgcggccg gatttaattc
     4441 agaaaattga cttagtgtat caaaaggttt aaagaattga ggaagttgtg aggcaagcga
     4501 gtaaacaaaa tgatggaaag gaaagtggcg gcggaaaatt tctttgaaat tttccgccgc
     4561 cactttcatt gtttaaaata cgggtaaaaa attatctgaa tgattgacgc agttagaaga
     4621 gatcttttct ggctatcttt atgagaacaa caaaaatgaa aaatttaatg tataaaacct
     4681 cgattttttt atttgctttt tggtaattat tttgatacat taaaagagat agtttaaata
     4741 ataaaaatcc aactaaaatg gcggatcaag atgaagcaaa aaaactgagc catatattag
     4801 aagggacgag agaagcggcc gaagagcgag aagctgaaag gttggctaaa agtcttaaat
     4861 tccaatatgc caatctgaca aaatttgctt ctcagactca ggcccttcag ttaattaacg
     4921 aggaagaggc gcgagcagct ttatgcgcgc catttaatgt tcaacaaaaa aaggtatttt
     4981 tagcgtgtaa gaatccagcg cttgacgcaa caaaaaacgt gattgatagt ttaaagcaac
     5041 gaggatatga agtggagata tttgtggttt cttctaaatc gttggagact ttgtggaaag
     5101 gatataagtt tattaccggt cggaaaaaag aaattaccgg tggtttggaa attgatgcta
     5161 atcgattggc ggaactatcg agcgagataa aaaatattgc cattttgaaa gaaaaaattg
     5221 ataaatttga tagtccttat atttctcaaa tctttgaaat tattattgca ggcgctttaa
     5281 caatggatgc ttctgatgtt catattgagc cagaagaaag cggcgtgagg ttaagatatc
     5341 gattagatgg cgttttgcaa gatattgttg atttgaatga acattattat cgctcaattt
     5401 tatcaagaat taaattgctg ggtgggatga agattaatat tcataccgcg cctcaagatg
     5461 gacgttttac aattaaaaca aaagatttag aaattgaagt gcgtgtttca attattccga
     5521 gtgcgtatgg tgaaactgtg gtcatgcgct tgcttgatcc acggagaatc caattagatt
     5581 taaaagaatt agggtttcgc gaagacgact tagttattat tgaagagcaa gttgctcggc
     5641 ctaatgggtt gattttgaac actggaccaa ctggttcagg taaaaccact accttatatg
     5701 catttttgag acgcatttat caaccaggca ttaaaattat caccattgaa gatccaatag
     5761 aatatcattt agaaggaatt actcaaacac aagttaatcc cacgagagat tatacttttg
     5821 ctaatggttt gcgttctatt ctgagacaag atccggatgt aattttggtg ggagaaattc
     5881 gtgataatga aacagcggaa attgcaatgc atgccgcatt aacaggtcat cttgtattat
     5941 caacgcttca tacaaatgat gcgtgcggta ctatcccgcg gcttttagac atgaaggtgc
     6001 agccagcgat tttggcgcca gctttaaatt tagccattgc tcaacgattg gtgagaaaac
     6061 tttgtgttca ttgtcgggaa aaggtagagg ctacagcaga gcaagctgaa aaaattcgta
     6121 aagtaataga acggcttccg gatcgagtta agaaaccaac actcggcaaa caaattactt
     6181 tttacaaatc gaaaggttgc gaactttgta atggtacggg ctataaagga cgaatagcga
     6241 ttgcggaatt attagtggtt gatagagaaa tccaggattt aattaatcaa ggcgatcgta
     6301 tgccgacggc gaatgaaatt aaggatttgg caattaaaaa ggggatgact acgatggaag
     6361 atgatgctat tttgaagatt ttagatggaa ttaccagctt atctgaggca gaaaatgtgt
     6421 tgggaaagtt gttataaaat tcaaattgtg taatgtataa ttgaaaaagc ggcggaaatt
     6481 ttctttaaga aaatttccgc cgctttttca tccgtcatca aaattatttc atgaattaat
     6541 tcatgaaata ataaacaatg aaaatgctaa tttagtatca attcttttat tttttaaaaa
     6601 aattaaaata gtgcattggt tttgaaattc aagttttatt tttgtcattc ggaatgaaag
     6661 aagtagtttt taaaatttga agtgatataa tatttttact tgcgtttaaa aaaagcagat
     6721 ttatcttgtt gtcaaaaaaa tgaagttcaa ataatcgtgt cagctgtttc tgtgtttttt
     6781 aagttttgtt ttaataaaaa acagcccctt caacccggag gcaatttaat ctcgcaaaaa
     6841 agatgcgagc tcaagaattt tttaaggcct acccgctgac aggcgagtat gataacccag
     6901 tcggaaccag gttaccctta aataaattcc ctataggctt taattgggtt gaaataacgc
     6961 caattgcccc caggttgaaa aggctgtttc gggtaactat ttttaagaga acaataatcc
     7021 tataatgata ttatagcaca cctaaagata aaagtcaata gtttgacgga tatcgtaaaa
     7081 atggttaaat aactaaaaat taaagaagta aaatgtatta tagtgtttag ttattttaat
     7141 gtcaagaggg gagttgggga taagtttagc cccccaaaat tataaatttt agattcttca
     7201 ataaggtaaa ttactgcttt aatattttcc aagcgttata tttattatta gagttgaaga
     7261 tcaactaatt tttacacttt gacttttaag attttgatta atataataaa agtattacca
     7321 tgtctaagaa tattttcaaa tcggatccac tacttgataa tgccctgcga ccaaaacgat
     7381 gggaagattt tatcggtcaa gaaaaagtga aaaaaaattt aagaattatg ttgggagcgg
     7441 cgcaaaaaag agaagaggta gtagaccacc tgctttttta tggcggagca gggttgggaa
     7501 aaacaacact agcttattta attgcagaag agattcatgc caatttgaaa attacatctg
     7561 gtccagcaat tgaaaaagca gcagatctag catcacttct cgctaattta gaagaaggag
     7621 atatattatt tattgatgaa atccatcgtt taaataaaat gattgaagaa attttatatt
     7681 ctgctatgga atcgcgggtt ttgaatttag ttttaggcaa acgttcttct actcgcgctg
     7741 ttcaattaga tttgccgcct tttactttaa ttggtgccac aacaagaatt ggcttactct
     7801 ccagcccctt acgttctcgt tttggagcta ttttcaaatt ggatttttat gatttagaag
     7861 atataaaaaa gattttgcaa aacaatgcta ggttgctggg tgttaatatt tcggaagagg
     7921 caatggaaat tttagctcga gccagtcgag ccactccgcg agttgccaat cgtttgctca
     7981 aacgaagtcg cgatttggcg acaatcgaag gtaagagcga gattgatgct gcaattagcc
     8041 gcaaaatttt agagttattt gatattgatg agtggggatt agaagcaaca gatcgaaaga
     8101 ttttagaggt aattgttgat cgatttaatg gagggccagt gggcattaaa gctttagctg
     8161 ccgcgaccaa tgaagaagaa gatacgattg aagaaattta tgaaccatat ttaatgcgat
     8221 tgggtctttt agagcgtaca acacgtggcc gaatggtgag cgatgccgga tataaacatt
     8281 taaatcttta tcatttagca cagaaaggcg aacaaaagaa attgatagat tgattataat
     8341 aaaattcaaa agtccgccgg ggacgagtaa attcttttaa ttaaagatgg cggcggaaaa
     8401 ttttcgaaga aaattttccg ccggccgcaa attcatttct caaaacaacc aatcaaagtt
     8461 ttatatgttt aaacattatt tttcttttct tatagttgcc ttgagcgtct tgttgttttt
     8521 tcctttggtt caagcactgg ctgtggggcc ggtgattatt aatgaaataa tgtatgattt
     8581 acaaggaaca gacacagatc atgagtgggt agaaattaaa aatatttcag atcaagcagt
     8641 tgatttgaaa gattggcgtt tttatgacgg gagcaatcat ttattaaatg aacctcctca
     8701 aaatggcggg cagggcagtt taattattgc gcctggtggt tatgctgttt tagctgacaa
     8761 agcagatgtt tttttagctg atcatccggg ttttggtggg atcgtgattg atacggtgat
     8821 gagtttgaat aatactgagg aaaccgtgca acttattgat caaagcggca atagtattga
     8881 aaaaataaac tatcagaagg aaatgggtgg caacggcaat ggttattcgt tggagcgtac
     8941 gggtgatttt tcgatgcaat tttgtgaatc taatcaatta ggaggttcgc ccggccaagc
     9001 taataatttt gattgcaata aatcctcttt tatgccgact ccttcgttga cagcgatgaa
     9061 tacgcctacg ctaatgatgg caactgtttc tcctagcgct tcccccgaaa cgattttaga
     9121 agatacgatt attgaaagtg aagataattt gttgcgaacg acgcccattg cttcttcgcc
     9181 agtgataaaa gtaagcatta ttattaatga atttttacct aatccaaccg gttctgatac
     9241 agacaatgag tggattgaat tatataataa cagcgatgtt aaagtagcgc ttacagattg
     9301 gtggattgaa gatgcgagcg ggcagaaata tacttttaaa gatgaagaaa taatgagtca
     9361 gggttattta acccttcctt attccaaaac gaaaattact ttaaataaca atggtgaaac
     9421 tttgagttta tattcacctc gaggagaatt agcttttaaa atttcttatt cgggcaaagc
     9481 caacgagggt atgtcttttg cgcgagccgg ccaaaatgac tggcgatgga cttcaattct
     9541 tactcctaat aagcagaatc aattttcaag cgaatcatcg caaaatacgg aattagcaat
     9601 tcaacagatg gatgaatcgg atgttgctga aaataatgcg ccttcaactg ttgatgccga
     9661 ctcttcttta aagcagcaga cagtaacgag ttcgatatca aacgcgtcag ataagataat
     9721 tattgttgct attggattgg gtttaatatt aagtgtagta gcagcaattt ttattaaaaa
     9781 gattttaccg ccagttcatt gatgaaattt tattttaaat ccgctagagt ttctcctgtg
     9841 taatactgag cttttaacgg cacggaaaga aattcatttt gctccattaa aggaaggaga
     9901 ttgtttattg tccattcaac tttgttttct ttgacttcaa aaattagctc atcatgaatt
     9961 tgcaaaatga gacggatatc gtcaattgca ttttcttttt gaagaaagtc attaattttg
    10021 atcattgctt ttttaatgat gtcggcggct aatccttgaa tagggaagtt gatagcagcg
    10081 cgttcggcaa ggctgcggta tttttgattg ccagagatca aatcccaaac ccagcgaatg
    10141 cggccgctta aagttttaac gcagccagtt tgtcgtgctt cggccaaagt tttttctctc
    10201 cagtctttaa tattggcaaa atcatgataa tattcttcaa tgaatttttt agcttgatct
    10261 tttggtattt gagcagtttc gctaaatgct ttagcgccca tcccataaag aatgccaaaa
    10321 tttaatgttt tggcttggcg gcgcattgca ggcgtaactt gatcaagggt ggtgttattt
    10381 atttctgccg ctgttagagc atggatatct tggtcattaa gaaaagcctc tttcattttg
    10441 ggatcatcgg ccaaagaagc tgcaatacgt aattcaattt gagaatagtc aaaaccaaca
    10501 aattggtagc ctttttcagc aacgaaacct tgtctaattt tttttgccaa ttctccttct
    10561 atcggaatat tttgaagatt gggattttct gaagagaggc gaccagttgc tgtacctgtt
    10621 tgattataag tggtatgaat acggcaattt tcatccatta aagatggcaa cggttctaaa
    10681 taagtgcttt tgagtttcat taattcgcga tattgaagaa ttttagcgat agccggatgg
    10741 agatctttaa ttttgatgag ttcattttca cgagtagata aaagcccagt ggcagtttta
    10801 gaaagatgtt tgttgttcaa attaagtttt tggaaaatta cttgagaaag ttgtttgggg
    10861 gaattaatat taaactccga attcgttagc tcaaaaatct ctttttctaa tttttgtaat
    10921 tcgtgattta attcatcggt taagcgataa aaatgatccg gattaatttt aatgccgaaa
    10981 tgctccattt gcgccaaaac attaacgagg ggcatttcaa tttgttggaa gacatttgtt
    11041 aaagcaagct ctttgatttt atttacctgc gctgttttga tttttttcag gattgttaaa
    11101 atattatcgt tgggaagagg ggttttgaaa atgatttgcc aaattctttg aagggagtaa
    11161 tcttttaatt cagaattgat gagccattga gcaattttaa tatcatggtt taagttgaga
    11221 ttgtaatcat caatttgttt tacaatattt tttaaatcat aacccacgag agaaaattgt
    11281 ttaattttat taaaggaatt ttgatcttta ggtaaagaaa aaaattgatc ggctactaat
    11341 aattctacat tcttattgag attaataatg gcttcggctt gagttggcag aggatttaat
    11401 ttttgttctg tttgccattg aatagaggct tgagagaaag cgttctctgg cgtattggtt
    11461 tgggactctt cgggcattaa ttgatttaca agactttcaa atcgccattt tttaaaaaga
    11521 ggaatgagtg attctttgtt aatgccatta aattttaaat cagaaagttt tacttctaaa
    11581 ggagcattgc aatttagagt tgttaagtat ttactaaaaa atgcttgctc tttttgatct
    11641 aagagcgcag ctaaagtttt tggtttaatt aagcgcgctt cagaagtttt ctgaaaattt
    11701 tcattttcaa ttttttggta aaggttttct aaggtttcaa aatgtttaat taattcaata
    11761 gctgtttttt ctccaattcc tttgacgccc aaaatattat cagaaggatc gccttttaac
    11821 gccttaaaat caattaactg tttaggacta aggccatagc gttcctgtac ttccttttca
    11881 ttataaataa cggtgtcttg aattcctttt ttcattgtgt aaaccactac tttatcatcg
    11941 tctactaatt gaagagtgtc cagatcacca gtgagaataa taatttttaa atctttttcg
    12001 ttagagaatt ttttgacaag cgtgccgatg agatcatcgg cctcataacc gggaatttcg
    12061 aaatatttaa taggaacggc actaaaaaac tctcttactt tatcaaactg aggaataaga
    12121 ttttcatcag tttttgggcg agtggcttta tattctttat attctttatg cctaaaagta
    12181 ggttcggcca aatcgaagca tgccgcgaga taatcaggtt taaattcgcg gataattttt
    12241 aaaataatgc gaatagtgcc atacactgcg ccaataggtt cacctttagg agtggttaaa
    12301 tttggcaaag catgaaagca gcgatggatt aacgcatgag aatcaataag gataagagtc
    12361 ataagaaaat taaaattcaa aagatggagt caaaaataaa attatttaag ttttttagtt
    12421 ttgggttggg attttatttt tgaattttca acttttgaat taattgttat ttctctctta
    12481 tttttatcaa cgactttgaa attgatccac agagtatttt ttggaagtag ctttttaata
    12541 cgttctggcc actcaataaa aacgagattt tttggatcct gaataatttt tttaaaatca
    12601 agagtaagaa tttcctccgc tttttccaga cgataacaat caatatggta aaaattttta
    12661 aaatggggat tttttcgtaa tgaaaaagat ttaatgatta aaaatgtggg gcttaaaatt
    12721 ctgcctttga tttttagaga tttgcctacg ccttgaataa aggtggtttt gccagcgccc
    12781 aaattaccct gtagagcaaa aatatgaggt ggtgaagatt tttggtccag tagtgttttt
    12841 acaagaattt gagcaatttt ttgagtttgg actggtgaat gagaaagaat ttttagggaa
    12901 gattgcatta aagaaattat acaataaaaa gttaaaaaac aaaatgacga aaggcgaaag
    12961 gggcggcgga aaatttcttt gaaattttcc gccgcccctt tcgcctgtcg caaatcgttt
    13021 tcaaatacct atttttttaa tttgcttttt tctttatttt gtcttaaatt agaattgatg
    13081 agtcattttt ttgaaattga aggaagtaaa aaattaaaag gagaaattaa agtatcaggc
    13141 acaaaaaatg gcgcactggc tattttacca gcctgtcttt taaccgagga gccttgtgtg
    13201 attaaaaata ttccgctgct tactgacgtt ttagtgatgg ttgatattct aaagaattta
    13261 ggggcggaaa tagaatggtt agatgaaaga acaattcgta ttcaagcaaa aaatgtttta
    13321 agtgatgctg atacgaaatt agtaagaaaa attagagcat cgtttttagt ggctggtcct
    13381 attttagcgc ggataggcga attaaaaatt gcaaaacccg gcggttgtca tattggcgcg
    13441 cgcagtttag atacgcattt tgagggtttt gctgatttgg gtgttaaatc agctttagaa
    13501 agagaagaat ctgaaaaata ttatttaaaa gctccaaaac aatttaaacc agccattgtt
    13561 tctttaaatg aatttagcgt gacagcaacg gaaaatctaa tgatgttaat ggcgaagatt
    13621 gaaggaaaaa gcgaaatcca tttagcagct gctgaaccgc atgttcaaga attaggcgaa
    13681 tttttaaaat caatgggcgc tgagattgaa ggattgggaa cacataccat tattattaaa
    13741 ggcaagcaaa aattgaaagg cgcagaacat accatttggc ctgattatat tgaagcgggg
    13801 acttttttta gtttagctgc tacaactaaa agtaatctta ctattaccaa tgtacctgtt
    13861 gcttatttag aaatggtctt tcgtaagttg aaaaaaatgg gagtttcgta tagaataaaa
    13921 gacagccagg ttttaattga gggcaaagat agttctttga aagcagccaa aattcaaaca
    13981 ttaccgtatc caggatttcc aactgatctt caagctcctt tcgcggtttt agctactcaa
    14041 gctaaaggtg acagtttaat ttttgataca ttatttgaag gcagatttaa atatgttgat
    14101 gagttaaatc aaatgggagc aaaaattttt ctttgcgatc cgcaccgtat tcttgtatca
    14161 ggtccgacgc cgctttatgg aacaaaaatc aaaagttttg atttacgttc aggagccacg
    14221 ctgattattg ctgccttagc ggctcgggga aaaagtcagc ttctaggcat tgaagaagta
    14281 gaccgcggtt atgaaaaaat tgaagagcgg ctgcaaaaaa ttggtgcgaa tataaaacga
    14341 gtcgaggcag aatcataata atatgtttat taaaaaaatt ggaattgatt tagggactac
    14401 aaatacattg gtttttgtac ccaaaaaagg cgtaattatt tttgagccaa ctattgttgc
    14461 ttatgatctc aatgacaata agatcttagc ggtgggtaag gaggcaaagg aaatgttagg
    14521 acgtacgcca gatgatatag gagcttatcg gccattaaaa gacggcgtga ttgctgatta
    14581 taaaacgacc aaaattatat taaagtattt tatttctaaa gccttaggta gttttaattt
    14641 ttttaagccc actttaatta tttctggtcc agccggtatt acttctacag aaaggcgagc
    14701 ggttattaac gcagctttgg aggcgggtgc gcgtgaagtt tatcttgtcc gcgaacccat
    14761 cttggctgct ttgggggcgg gaatccccat taattccgct tcaggtcata tgattgttaa
    14821 tatcggaggt ggcactagtg aaattgcggt gatttctttg ggtggcattg tttcgtgggc
    14881 ctctttaaga attgcaggga ataaatttga tgaggcgata gtgaattata ttcgaaagaa
    14941 atataattta gcgattggcg atcaaacagc agaaagaatt aaaattgaag caggcgcagc
    15001 tctgcctcaa aaaaacaaga ttgaaaccga agcaaaagga cgtgatttaa caacagggtt
    15061 gccacgttct gtgatcgtca attctaatga aattgctgaa gcactggctc ctcatttgga
    15121 agaaattgcc gaaagtattc gttctgtatt tttgaatact ccaccagagt tagcagcgga
    15181 tattattgaa aagggaattg tgattgctgg cggcagttca ttgttgcgag atttagattc
    15241 atatatttcg aaagttacgg gggtgaatac ttttctagcc gaagatgctt tgtattgtgt
    15301 agccaaaggc acgggcatta ttttggaaca tttggatatt tacaaaagag ctctaatgag
    15361 caagcgttaa aatgaaaata gatcaatatt tcaaaaaaat tattgagcaa gatataattg
    15421 atggcgcttg gcttttttta agcatggatg aggagatgct ttttcaaaaa agtttaagtt
    15481 tagccaaaca agttacttca gcatcttata tttatattgt ttccgagagc gagcaattgg
    15541 aagattttat taaaaatagc catcaggttt ttgatccggc aataaaaagc atttttgaaa
    15601 ttcaaagatt gcttttttta tctagtgatg aaaaaaaagt ggtaatagtt aaaatgatgg
    15661 aagatctttc tgaagaagcg caaaactctt tgcttaaaat caccgaggaa ccgccagtga
    15721 atactgcctt cttttttctc agcaaagacg agaatagagt attgccgaca ttgcgttcgc
    15781 gttttcggat aattaaattt cctgaaaaaa attcctttta ccaaaaacgc atttcttcgg
    15841 aaatgagaac gtttctacat tcttctcagc cgctcaatgt ttatttagaa aattttttgg
    15901 aagaggatcc ggtaattttt ttagaaaact tagtcattgt tatgagggat ggcctcttga
    15961 attccttaaa tcttgaggag ttaaaaatta caaatggaga gggagaggca aatgtaaaag
    16021 atttaaaaaa cgccctttac gccttagaag gtattaaata tcataattta aattctcgcc
    16081 tgcaaatcga agatttatta ttaaatatta ataaaaatta atttttttat ttttgttgct
    16141 aaaagccatg ggatccttcg ggcgttaccc tcaggatgac gtaagaagcg aaagccgaag
    16201 gatccttcgg actaaaaccc tcaggatgac aatgtattgg tttgtcattc tgagtgaaac
    16261 ctgcctgccg gttaggtagg tgaagaatct catactcagc agtgatggtt aataaggcgg
    16321 cggcgaattt cttttagaaa ttcgccgccg gtaaaccctg ttttattttt aaattttatg
    16381 ccactgcttt attgctaatt atagagtgaa aatttgagat ttacatttta ttatattata
    16441 attattatag tcgttaaaac atttatttat gcccattaat caattaatcg aaaaattaaa
    16501 aaaagaaggc gaatctgtga ttgaaggatt tcggaacgaa attttaggtg ttcgttctaa
    16561 tcgacctcag ccggctctta ttgaaaatat taaagtagaa tgtcctgttt atggttctat
    16621 cctgccactt aaacaattgg caaccattca agtgagtttg cccaatagtt taattgttca
    16681 agtatgggac aaagtaaatg tttcggcttg tgagaaagct attcaagcat ctaatttggg
    16741 ggtttctaca atagtagaag gatcgcaaat tcgagttatt cttccgccct tatctcagga
    16801 aaggcgagat cagattttcc aattaatcaa gaaaaaatca gaagatacaa aaattcgttt
    16861 acgtctttta agagaagatg ttttgaaaga aataaaagag ttatttgaac aaaagaaaat
    16921 ttccgaagat gataaatatc gtagcaagga gcagattgat aaattaatgg atgattttaa
    16981 tcataaaata gaggaaatcg ttgaagtcaa aaaaaaggag atttacgaat aaaagaaatt
    17041 aattttttca taaaaagaac cattcttttt atatgagtgc tattattttt atactttgtt
    17101 tagctttttt aattattgtt catgaatttg ggcatttttt agcagccaaa aaaagtggaa
    17161 tgaaagttga agaatttgga gttggattgc ctcctcgtat ttggggaaca aaaaaagggg
    17221 aaacgattta ttcgattaat gctttgcctt ttggaggctt tgtaaaaatt tatggagaag
    17281 atggttctta tgaaaatgaa gaaaatagtt tttcagcaaa aaaaccagct gttcgattaa
    17341 aagttttatt agcaggcatt gcaatgaatt tgatagttgg cattattctt ttgggtgttg
    17401 gttttaatat tggcattcct gctcttgcaa ccgaagataa tatgccttat ttacaagata
    17461 tcaaactatc tattttagaa gttcaaaaag attcaccggc agccatggct gggctcaaaa
    17521 caggcgatcg aattttagag gtgagtcgcg gtgatgaaaa aatatctttg cctacagttg
    17581 aggaattgca gtcgtttacg aaaaaatggg gcggtgaaga aattaattta aaagttgaac
    17641 gagggaaaga atcttttgaa attaaagcaa cgcctcgtaa aaactttgaa gagaatcaag
    17701 gggctttggg tattgcgatt ggcgaaacag gaatacttaa ataccccttt tttcaaagta
    17761 tttgggaggc catcaaagga ggatttagta tttttattaa tgtttttata gggctttttc
    17821 tttttttgaa aaatttaatt attaatggcc aaatgatggg cgaagtagct ggaccggttg
    17881 gcatagtggc tttagggggg cagacggtca aaatgggtat tggatatttg attcaatttt
    17941 tagcgacttt gtcaatatat ttagccgctt ttaatcttat tccttttccg gcgcttgatg
    18001 gtagccgcgc gctctttgtt ttaattgaaa aaattagagg gaaggctgtg cccgcaaaag
    18061 tggaaaattt aattcattct ttaggttttg ttattttgat tggtcttgtt attgtcgtta
    18121 ctttccacga tattttaaac ttattttgag gttaaaaatt gaaaaaagaa aaaaaagaat
    18181 tacaatatta aggactatgt tagaatcaaa agcatttgtt tcaattaatc gaggagctgt
    18241 tgaagatgaa acagctttga atgcacaact cctgataaga ggaggatttg ttgagaaggt
    18301 aatgccgggt atttatgttt atttgccttt gggctggcgg gtttataaaa aaatagaaac
    18361 aataattcga acggaaatgt ttagaatagg aagtgaggaa atttttcttt cagcgctatt
    18421 accaaaagaa ttatggataa agagcggacg atgggaatct tttggtgata ttctgatgaa
    18481 agtcaatgat cgtgatggca gggaatatgt tttgggccct acgcatgaag aattaatagc
    18541 agaattagct aaaaaataca ttttgacata tagagattta ccaaaagcca tttttcaaat
    18601 tcaagagaaa tttcgagatg aacaagaagt acaatcagga ttattaaaaa ctaaagaatt
    18661 tataatgaaa gatttgtata gttttcatgc cacccgacaa gacttggatt ttttctatga
    18721 ggaaatgaaa aaatcttata ttaatatttt taatcaatgt ggtttaaaat cttatattgt
    18781 tgaagcagcc ggtggtaatt ttactgatga gaatgttcat gaatttatgt taaaaacaga
    18841 agcaggagaa gagacgacgg ttttttgtga attatgcgat tttgctcaga acaagcaatt
    18901 gaaagaagca gaaggaagag atcaatgtcc tcgttgtggc ggtttactta aagaagaaaa
    18961 aacagtggaa ctgggaaata ttttcaaact tggaacaaaa tttagcgaaa acacaggagc
    19021 gtattttgtt gatcgtgatg gcgagaaaaa accaatttta atgggctgct atggcattgg
    19081 cttaagccga ttgatgggag taattgctga ggtttatcat gatgaaaagg gcttggtatg
    19141 gcctgtttcc gtagctccat gcctcattca tttaataaaa ataaacgaga cggatccgaa
    19201 aattgatgct aagataaaaa agcaagcaga taaattatat cagcaattga ctcgaaaggg
    19261 tttcgaggtt ttatatgatg atcgagttga gtttacaagc ggagagaaaa ttgcggaggc
    19321 tgacgctatc ggaattcctt atcaaataat aataactgct aaaaacttac aaagggaatt
    19381 agtagaaata aaaagtcgaa attcagggga aagcgaatta ttaaaaatat cggcggtcgg
    19441 aaactatttt aaaaatatta atcaataatt atgtttaaaa aattattagc aaaaatgtcg
    19501 ccgggattgg gaattgattt gggtacagcc aattcccttg tttatttgga gaaacaaggg
    19561 gttgttatta atgagccgtc ggtagtggct tacaatcaaa aaacgaatga gattttagct
    19621 attggacgtg aagcgcaagc gatggttgga cgtacgcctc ctcatattgt agcagttcag
    19681 ccattgacca atggagtaat ttctgatttt gaattgtcgg aagaaatgct tcgttatttt
    19741 tatcgtaaat tccaaaaatc caaattagga attttgccgt atcggccgcg ggtggttatt
    19801 ggtattcctt gtggaattac tgaagtggaa aaaaaggcag tagaagatgc agccttaaat
    19861 gccggagcag gcgaggttta tttggtagag caaactttag cagcggcgat cggtagtcat
    19921 attcctattt ttgaaccatc gggcaatatt ttagttgata ttggtggagg aactacggat
    19981 atcgttgtta tttcccttgg cggtattgtt cgttataaaa acttaaaaat tgctggtcaa
    20041 aaatttaatg aagacattat aagatatgtt aaggacgaat atcgtttatt aattggagaa
    20101 gtgagtgcag aacgtgtaaa gttggaaatt ggatcagcta aaattaatgg aatggaaaaa
    20161 ttagaaacag ttgtcagagg aagagattta accactggct taccgaaaga agttactcta
    20221 acaagcgaag aagttgcaag ggctttggat aaatccctga aagttttagt ggaagcgatt
    20281 cggtctacca ttgaggaaac accaccggaa ttagtggctg atatttctat aaaaaacatt
    20341 tggttgtcgg gtggaggaag tttattacac aatcttgatc gtttgatcga aaattattgc
    20401 atgatgccgg taaaaagcgt tgatgatcct ttaacggcga cggtgcgagg attggggatt
    20461 atcgtggaaa atttaaaaca gtataaaaat atccttattt atgagcacaa agagaacgaa
    20521 taatcttatt gtaattttgt taattattgc ctttctggga agttttatga ggccagtatt
    20581 ggctttagcc actaacgaag aagatagtaa taacggagat aaaactttta ccgcaagagc
    20641 tcgaactatt cttttacgtg ctgttcagga tattgataat tgggttagag gtttgattta
    20701 ttttaaggaa attcaagagg aaaataatgt tttggttttt caaaaccgag ctttaaagtc
    20761 tttgttaatt gattatcata atttaaagtc agaaaatgaa ttattgcgag aggcattaaa
    20821 aattcaccaa tcgcaagaca ttaattttct tttagccaac gttattggtc gatcgccttt
    20881 gaatttctct cagacttttg tgattgatgt gggagaggaa gatggaatta aggtgggaca
    20941 gagtgttgtt tggggaggaa aaactttaat aggagaaatt attgatgtgc gaaaaaattc
    21001 tagcacagtt cgagctatta cggatagtga atttagagcc gcggtttttg ttggtgaagg
    21061 ccgaactgaa gcccttttca ccggtaatgg ctttttacct ccttttttag atttggtacc
    21121 gacgaaagaa gaaattgctt taaatgaaca ggtttatact tcaggtttgg ataataaatt
    21181 tattcgaggt ttgtacgttg gagatgtaag tgaaattaat aaacccgaag gtaaaatttt
    21241 tcaggaaatc aaaattaccc cggctttaga ttggacgagc atttatcaag tcttagttat
    21301 cgttcattaa taatgaaaaa gataaaacaa attttgcacg ttcttacttt tctgttattt
    21361 ataagccttt tggccatttt acaaataacg attattaatc aatatcccat cggaggagta
    21421 aaagtaaatc tagttcttat aggattgtta gctaatattt ttaatcctcg ttttggaaag
    21481 gtggttttag gtgctgttgt aggaggtctg atttatgatt ttggcgggat ttttaatttt
    21541 atttctctaa taagtttttt ggttacaata gtaattctgc atttatggag taaaaaatat
    21601 ttggttaaaa agaacatgtt gatgattttt ttctttggcg ctattggaac aatatttttc
    21661 aatttttttt acgtgctgtt gagttatttt ctttttcatg caaacttttt ttattatttt
    21721 ttaagtctga gacacgttgt ggaaatatta cttaatggcg gaggagcttt tatttttgct
    21781 ttaattggtg agggatttac gcaaataagc catttattta aaaaataatg aaattaaatt
    21841 ttaagaagaa taaaaaagta aaaagagaaa gtgatttttt ggaagtagaa gaaattttat
    21901 ttaataaaga agcggtttca aaattaaaag aagaagaact tagtcgtttg gaggtttcta
    21961 ttgggaaatg ggggttaaat tcgatttttt tgtttgggat ttttctttta atttttgcgt
    22021 taggcttggt tggttattta aattttgcga aaggcgatga atatcaacaa attgcccaaa
    22081 aaaatgcttt aaaatccatt cctattattg ctgatcgagg tttgattttt gataatcata
    22141 atagggtttt ggtttacaat caagaagtat ttgatttagt gtttttccct gcgtatttac
    22201 ctgttgataa aatagaaaga gaaaagatat taaaaaaaat tgaagaaaaa ccagaaattg
    22261 cagcggctat taataaagaa aatttagaag aagatgaccc tttagagagt attattttac
    22321 aaagcaattt aagtcgcgaa gaggctatta gatgggaaag ctattttgct ggttattcag
    22381 cagttcaagt tattaggcaa aacttacgcc aatatacttc accacttgct tttagtcata
    22441 ttttaggtta tgtgggtcgc gtatcggctg aagatattca gaaaaatcca ctttatagta
    22501 attttgtgaa aattggcaaa gctggtctcg aatcttttta tgaagataat ttacgcggtc
    22561 aagacggaga aattaaagta ctaagaaatg cttatatgga aattttaggg cagagcgtcg
    22621 ttaaagagcc ggtggcgggt aataatcttt atttaacgat tgatgccgat ctccaagaat
    22681 acagctataa aactcttgct cgacagattg ataatttagg gaaagaaaga ggcggcgtag
    22741 taattgtttc tgacccgcag agtggagaaa tcctttcatt agttagctac ccaggttatg
    22801 atatcaatag tttgtcaaag ggtattaccc agaaagaatt tgaaacatta atcaattcaa
    22861 aagttactcc tctttttaat cgaacggttt ctggtcttta taatccaggt tcaacaatca
    22921 aaccaatcat cgccattgct gctttagaag aaaacatcat cgatcctttt aaaaaaatag
    22981 aaacgcacgg ctatattact attcctaatc cttattttcc tgatcggcca tctatttttg
    23041 ttgattggcg taataatggt gttgtaaaca tgcaagatgc aattgctcga tccagcaacg
    23101 tttattttta tattattggt ggtggttatg aagattttgt tggattgggc attgagcgaa
    23161 ttagaaatta tttagaaaaa tttcaattta atctagtaac cggtattgat ttaccagcag
    23221 aaaaagttag caatattcct gatgaaaaga gtaaaaaccc ctggcgtata ggtgacacct
    23281 acaatgtttc tattggccag ggcgatttat taacaacacc gataagatta ataacgagtt
    23341 taaacgttat gattaacggt ggaaaaattt tgcggccgta tcttttagat cacattgctg
    23401 ataataatca gcggacaatt tttcaaaacg aaatacaagt agcaaaagat aattttctaa
    23461 actccaaaaa catagagatt gtaaaagaag gaatgcgaaa aacggttacg agtcctttag
    23521 gcacggcgca tactcttatc gatttaccat ttagtgttgg aggaaaaagc ggcagcgctc
    23581 aaacatcagg taatacaaaa accaatgcct tattttatgc ttttgctccg tatgacgatc
    23641 cacagatttc cgttttaatt ttaattgaag atgtaccgga aggttcttta aatgctattc
    23701 cagtagcaaa agatatttta atgtggtatt ataaaaatag aggttttaaa taattaatgt
    23761 cttaatcgat atcttaaatt ttaaaatcta acctcaaata attttaaagg cattaaaaat
    23821 tataaaatta tttcggtgat gcttttagaa taacaattgg attgaaagcc gcgggattca
    23881 tctattagat tttgtttttg gatgctgcgc ggtggcgatt ttctttagaa aatcgccacc
    23941 gcgccgcttc atctctagtc gtttattact tttagattta atatttcatg aataatatgc
    24001 ccccagttta tttaagcaaa gaaaaatatg aagagttatg tcgtgaactt gaagcacgga
    24061 aaacgactat tcggcaaaaa atagccgctg atttaaagca tgctaaagac ctaggagatt
    24121 tatccgaaaa cgccgcttat acagaagcca aagatgcaaa agaaaaaaac gatcgtcgaa
    24181 ttgatgaact agaaaatatt cttgctcatt gcgaaattat tgaaaaatca caaaaaaatg
    24241 atagagttta tataggagct aaagtgaaaa ttcaagacga aaaaggagag attttcgaat
    24301 atcaaattgt tggcccagaa gaaagtgatc catttaaaaa cctgatttct tacagatcgc
    24361 cattaggaat ggagttgatg gataaaaaag aaggagattg gattaaaatt caaagcccca
    24421 atcaaacgat caaaaaatat aaaattatct ctattgatta aaattatgtc aacagctgat
    24481 caggttaaaa aaataagaat tgcaaaaata aaagaattaa aaaaacaagg caaggaagtg
    24541 tatcccagtc attgttttcg taaccaatca attagtaata ttttgaataa tttttattct
    24601 tggagtattg caaaaaagaa aatttgggta ggaggaagaa taatgagtat tcgttttcat
    24661 ggcggaatag catttgccga tttgtgggat gccagcgggc atttacagat tgtttttaaa
    24721 aaagaggaaa caaaaaattt tgaacttctc aatcaatact tggatgtaag cgattttatt
    24781 gaagtaagag gttttccttt caaaacggaa agaggggaaa aaagtttatt agttcaagag
    24841 tggcgtattt tagcaaaaag cttaaagccc attccttcag aatggtatgg tttaaaagac
    24901 accgaagaac gctttcggcg tcgctatctt gatttaattt taaacgagga agtaaaaaag
    24961 cgctttgaaa ctcgctcgag aattattaaa gcaatgagag gcttttttga taaggaaggt
    25021 tttatggaag tggaaacgcc tattttacaa acattggccg gcggagccgc tgcgtgtcca
    25081 ttcaaaacaa aacttaatat tttgaatatg ccactttatc ttcgcattgc accagaatta
    25141 tatttgaaac gattaattgt tggtggtttt gaaaagattt ttgaaattgg taagaatttt
    25201 cgcaacgagg gtattgataa aactcataat ccagaattta caatgatgga gctttattgg
    25261 gcctatgctg attatgaaga tatgatggat tttattgaac agctttttgt ttatattctc
    25321 aaaaacgcct tgcctcaaac aaaagacccc atgctcatta attttaatgg tcaattaatt
    25381 aattttactc caccatggcc acgcaaaaaa tttttagatt taattgaaga agaaacaagc
    25441 ctaaaaccgt tagcaacaaa gagtcaaaca tggagagcgt ttcttcaaac tcaaggagta
    25501 acaataacag aagaaataaa aaagatggat gagtgggggt tgatggatga agtttataaa
    25561 aaagtttgtt tagaaaaatt aactcagccc acttttgtta cccatcatcc cattgtttta
    25621 tcgccattag caaaattgag caaagacaat ttgaatgaaa cagaaagatt tcagttggtt
    25681 gctggcggaa tcgaattggt taatggttat tcagaattaa atgacccctt agagcaagcg
    25741 gaacgtttta aaaaacaaga acaacgaaga aaagcgggga ataaagaagc atcgccctat
    25801 gataaagatt ttatagaagc attggaatat ggaatgccac ccacagcagg cgttggtatt
    25861 ggcattgatc gattggttat gcttttaacc aatgcaccat ctatcaaaga ggtcatcttt
    25921 ttccctttta tgaaaaataa ataaaaatat gcttacagat ttattaaaaa aaattagaga
    25981 aaatcctcaa ttatataaag aaggaattgc aaagaaaaat tgcaacgtcg aaattattga
    26041 taaaatttta gcaattgaca aagaatatcg tagtaattta gcagaagtgg aatttttgag
    26101 agctcattta aatgagttgt cacaaaatgc taaatttagc caagatcatt tagagcaagc
    26161 gcgagctact aaacagcgta ttaaagattt ggaaagggaa ttaacaccaa tagaaaatga
    26221 cttagataaa ttactttatc agattcctaa tttaccgttt gaggatgttc cattagggaa
    26281 agacgagagt gaaaataaag tattacgaga agtggggaaa aggccaaaat ttaaatttga
    26341 acctcaagat tatttaacgc taacagaaga taagtggatt gatactaaaa gagctgctaa
    26401 agtagcggga acgcgcttcg gatatttaaa aaatgaggca gtgcttttgg aatatgcgtt
    26461 agtaagatgg gtaatgaatt ctcttaaaga aaaaggattt gtgccaatag tcccgcctgc
    26521 ttttatttct tctaaggcta tgaaggggat gggttatatt gataatgaaa aagatttggc
    26581 ggaacgttat tattttcctc aagatgattt atttttgatt ggtacagccg agcaagcagt
    26641 tggtccgatg catatggacg aagttttaga agaaaaagag ttgcctttgc gctatattgc
    26701 tttttcgccg tgctttagaa gagaagctgg ttcttatgga aaagatacca aaggaatttg
    26761 gcgcgtacat tattttgaaa aattggaaat gtttattttt tgtaaaccag aacaatctga
    26821 agaagagcat cagcttattt tagctattga agaggagttg atgcaaaaat tgggattgcc
    26881 ttatcgtgtt attcaattgt gtacgggcga tttgtcaaat ccttcggcta aatcttttga
    26941 tattgaagca tggttgccga gtcagaatcg atacgccgag actcattcgt gcagtaattg
    27001 cacagacttc caatctcggc gattaaatat tcgctatcga tccaaagatg gtttgcaatt
    27061 cgtgcacact cttaatggaa cggcgtttgc tattggcaga atcatcgttg caattataga
    27121 gaattatcaa caagcagatg gttcaattaa gattcctgag gttttagaac aatatcttta
    27181 atatcatatt tatgttatgc cttatcgact tacatcttat aaattaaggc agattcaaaa
    27241 gaaaaaacaa agaaggcgaa ctatttttgt tatcattatt attgttctct tcatttgttg
    27301 cctcggcggg atattatttt tttcacctct ttttcaaatc aacagcatag ctattaattc
    27361 tactcatttt gttaaaatga gcgatatttt tcagaaagca accgagcagt ttaaggatca
    27421 aaaactgttt aatttaataa ccgttccggc taacctcatt ctttttactc aagctgattg
    27481 taaggctatt gaaaaatatt tcgtggcttt ggattctgtt gactgccaaa aaatttatat
    27541 tgctaaaatg cttaaaattg aagcgcgaga aaaacaacaa ataggaatcc tttgttctga
    27601 ttcggtaatt aaaaaccatg gaaattgttt gtattttgat caaaacggta tcgtatttgc
    27661 tctagccgaa gaagacgatg attttgatca ttctcttatt gcgatgcaag atgttactaa
    27721 tcgcatttat caattaggtg ataaaattat ttcctatgat ttttcgcctg tggtaaaaat
    27781 aaaagacatt ttggcgaatc atcaattaat attgaataaa atagaaattg atgatcatga
    27841 tgtggtttat ttttttgaaa acggatttaa attgttaatt tctcaagaaa aattaaaaga
    27901 gacagcgaat attttacctc gtttattaga aatagaattc gatttacaaa aattagagta
    27961 tttagatcta cgctttttgc caaagatgta ttataagtag attgaaagat tagagtgata
    28021 aagatagggt ttttaagggg cgacgaaaat tttctttgaa aattttcgtc gccccgtccg
    28081 tcgcatttat tttcttgatt tagccactgt taagccaatg atttttttag agccattttg
    28141 ttttaaaact cgcgcagcct cttgcattgt agcaccagaa gtaaagacgt catctataag
    28201 aatgatattc ttgtttttta aggaattttt atccttaact tcaaaagcat ttttgagatt
    28261 taagtggcgt tcctcataat tatcaagttt tgcttgagga tgtgtatttt tttggcgtat
    28321 taaaatttca ctatttattt ctaattgaaa atagttagca aaaattttag ctatttcttc
    28381 gctttgatta aatccgcgtt ctttgaagcg ttgacgcgaa agaggaatgg cgatgacgat
    28441 tgctccagaa aattgttttt taagatcagg ccatattttt tcccagtagt taatgacgat
    28501 gttgccgagt gtatggcgaa tgtctttaac aaactgatat ttgtaaagat gaataagttt
    28561 ttggacgaga ggattttcat agtcagtagc cgaagcaata aaatccagag gggaataatt
    28621 attggagtgg gaacattttt tgaattggcc aattcttttg agacaaatag ggcagaataa
    28681 tgatgagtat agaggaatgg tttttaggca attttcacaa agatagagac cgctttcgtt
    28741 tttaataaaa gcgccgcaat ttaaacaaaa aattgggaaa aggcaatcat agattaaatt
    28801 ttttattttg attattttat tcattgtgga taagtataac ttgttgcttc caactatctt
    28861 ttaaattata atataataaa agagaaaatt aattctagaa ataaatttta tgcgcacttt
    28921 aggaattgat attgggacaa ctaatataaa agcagtggaa ctttctggga cactgaataa
    28981 tataatttta gagaattatg gtattttaga aacttacggt tatttagagc gtagcaatgc
    29041 tgctattcaa acgaattatt ttaaacttgt tgaagaaata acaacagaat tattaaggca
    29101 attattttca gcgttcaaac ctaaaacaaa aagagcattt ttttcattgc ctgtgttttc
    29161 tagttttatt acgaatttcg atttgccatt tactaataat aaagaaattg ttcaagcagt
    29221 tccttttgaa gctaaaaaat atatcccatt accgttggag gaattagaaa ttaattggat
    29281 ggtgatagca aaagctgcag atggaagaaa agaaaaaagc agaattgttt tggtggcaac
    29341 accaaaggaa ttgattcaaa aatatcaaca cattgctcaa acactcggat taaatctgat
    29401 gggttgggaa atcgaaggat tttctttagc ccgaacgctt gtggcagatg ataaaacgcc
    29461 aacattaata gttgatattg gcactcaggt tacaaatctt tttgtagtag ataacacttt
    29521 tttggttaat ttcgaaagtt taaatattgg aggtgctgaa atcactcata ttattagtca
    29581 aagtttaggc gttactcaag aacgagcaga agagtttaaa aaaattaaag gattcaaagt
    29641 taatccagaa gaaatgggtg ttgttaatgt attgatgcca attgtggata attttgggaa
    29701 tgaaatttta agaatgattg atatctatcg cgaaaaaaat aatcgtcaaa tagaaaaagt
    29761 cattcttaca ggaggaacgg ctaatatgcc aggattaacc gagtatttta ctgaattttt
    29821 gaatttgcca gtagaaaaag catggccatt taagcagata aaataccaac agtttttaga
    29881 gccgctttta agggatacag ctccattttt aagcatagcg actggtgtgg caatgaaggg
    29941 cttggaatag ttgtgaaaaa ggattataat aaaaattaga gggggagttg catttggaga
    30001 tttaaaatat aaaggttata ataaaagaaa taatatggca gattttcaag caatagaacc
    30061 attaaataaa aaaaagacaa caggatggat gactaattta attactgttg cagtaattat
    30121 ttttgttgtg gtttttgctt tatattttgt aggcgtgttt tatgagaatt atataacaaa
    30181 aaacatatcc gatattaatg ataaaattaa ggatttggcg aaagaaatac cgattgaaga
    30241 tcgaaatgaa gtattgacct tttattccca attaataaat cttagagctt tgttagcaaa
    30301 tcatttgtat ccatctaaca tcttttctcg tttagagctt atcacccatc ctcaagtaac
    30361 ttttaccacc ttttcttatg aaaataaaac ttcaataatt aagttagaag gatatgcgcg
    30421 tgatttaaat gttctagcac aacaactttt agctttgcaa aaaacaacag atttcgtcaa
    30481 aattacagtg aacgatataa aaaacgcagc agacaaagtg caatttggag tcgaaattaa
    30541 ttttaatcca acttttattc acaaataaat tatgaaagaa tcggaaaaaa gaactattag
    30601 tattattatt attttggctc tttttattgg agcggctata gtatttttta caatgacttg
    30661 gccgatgttc gatcaagttt taaaagataa tagaaatctt gctttggcga aagccgaata
    30721 tcaaagacag gtagaagcag ttcgattagc aaaaaccatc gtagaacagt ataaaaacct
    30781 aacggatgtt aatcagatga tttcattaac catgcctcga agtgaagagt tgtataatgc
    30841 aatagttcaa ttaaataaaa tttcagaaga tagtgggttg agcattcaaa gtattgcgtt
    30901 gcaaaaaatt gctagcgcta ctactgcacc tagcaagacg ttaagtacat cttctcaaag
    30961 cttgttaaaa tcaccacagc agttggctct gacactatct cttaacggaa catacgaatc
    31021 atttaaaacc tggttagaag ctgttgagac gaatattcgt ttgatggata taactaatat
    31081 ttctttttcc ggagttaaag ctacaccaac agagagaggg ggtgatgcag gtttcaactt
    31141 tttcaacttt agcgttgctt taaatttata ttatcaacca taattaatat ggaaactata
    31201 gaacaaaaat caaaaactaa tatctttatt tatattatca ttgcaatttt aattgttgct
    31261 attgtgggag tttattttgt atttttcgct cccgaaaaat tagcaacttc agaaacaccc
    31321 actgctattc ctgttactaa tacaactgtt gaagaggttg gttcgtctcc tgcgacaaag
    31381 actacatcag ctcctttagt aagccattcc acctccaccg atattactgg agggttaagc
    31441 tctcttaaat taacgccaga agatatttta aataatccca tctttcaatc attacgtgct
    31501 tatgctgaac cagttgaagt gccaacactt ggtcgtccga acccatttat tccttattaa
    31561 aaataattaa aataaaatga tcccaaacaa aattttatta gaacttttag ttaaaaataa
    31621 taaaattgat caggccacgg ccgacaaact tctcaaagat agccagacgc ttaatcaacc
    31681 agtagaagaa attatttacg aaagaaatct cgttcctcaa gatgaggtag caaaaattaa
    31741 aagcgagtac tataaaattc caacaaaaca tttcaccaaa gaggatatta ttcccaacga
    31801 agttttgaat attatcccag aagaggtagc gcgcacacat caaatagtgg cttttaaaaa
    31861 agatggagat actttgcacg ttggaatgct ttctcctgat gatcaacaag ctcaggatat
    31921 tttgaaattt attgccaaac aacgcaaact caacattgca atttatctag ttactaaatc
    31981 agacttggaa aacatctgga agaattatcg atcatttgct acgcgcttaa aagaaattca
    32041 agacagcgta gatattttca aaagacaaag caagccatct catcaactct atcagcagcg
    32101 cgtggtgcgg attgatgaag caagtggtat cattgctgaa gaggcgccaa ttattacgat
    32161 tgtttctact gtattagaag aagcagttga atcaaacgct tccgatattc atatcgagcc
    32221 gatgagaaat aaattacgca tccgttttcg attagacggc gagttgaaag aagtactttc
    32281 tcttcctttg gaattacaac cagcgattat tgcccgtatt aaaattcttt ctgatttaaa
    32341 aattgatgaa actagaattc ctcaagatgg acgttttaga actattttgg ataataaaga
    32401 aattgatttt cgtgtagcga cgtttccgac ggctttgggc gaaaaggtag cggtacgtgt
    32461 tcttaatccg tatgtcggct taaaaactat tgatgatttg ggagtgagag catatcataa
    32521 aactttgatt gaagaagcta ttgcaaaacc atttggaatg attttagcca ccggtccgac
    32581 agggtcagga aaaacaacta cactttatgc tattctccaa ggattaaata aagaagcggt
    32641 aaatattgta agccttgagg atccggtaga gtattttatt gaaggattaa atcaatctca
    32701 agttaaacca gaaattggtt atgattttgc ttctggttta agacagattg ttcgtcaaga
    32761 ccccgatatt attatggtag gagaaattcg agataatgaa actgcagctt tggcagtaca
    32821 cgctgctttg actggtcatt tagtgctttc aactcttcat accaacaatg caataggagt
    32881 aattccgcgt ttaattgata tgaaaattga gccatatctt ttaccagctt ctttgatttt
    32941 aatgatggcc caacgtttag taggaagact ttgtgatcag tgtaaacaaa aagtagaagc
    33001 aactggtagc ttgaaagagg aaatagaaaa atccatagct gcgttgccaa aagaattgag
    33061 ggcaaagtat caaaaagata aatatgaaat ttatttacct cagggttgca aggagtgtaa
    33121 ttataaaggg ataaagggaa gaatgcctct ctttgaagtt ctcaaaatga caccatcact
    33181 tgaagagatt gttttaaaag atccatcggc ggctaattta caacaagaag cagaaaaaca
    33241 aggaatggtt actctacgtc aggatggcat tattaaggcg ctcgaaggac taatttccat
    33301 tgaggaagtt ctaaaagaaa caatggaaaa agattaaagt taaaggcgtt taattttaaa
    33361 ttttatggca gatgtttttt ataaagaaaa aatagaaaat ttattaactt taacagcgca
    33421 acaaaatgcg tctgatttac atttttcagt tggtcgcaaa ccaacgttaa gaattgatgg
    33481 gcgtttagtt tctattgcca aggaagaagt ttttaccaaa gaagtggttg aaggattaat
    33541 tgatgctttg gtgccgtctg gccgaaagga agaatttttg aaaaatcgag aaatagactt
    33601 ttcttacacc ttatcagata aagctcgttt tcgagtaaat gtttattttc aaagggggaa
    33661 tatggcagcc gcgttaagat ttattcctta taaaattaga acaattgaag aattgaattt
    33721 accgcctatt ttacacgatt ttgcaaaata cagccaaggt tttattttag cagtaggacc
    33781 agcgggtcat gggaaatcaa caacgttagc ggctattatt gatgaaatta atcacgaacg
    33841 aacagcccac attattacta ttgaagatcc tattgaatac gtttttgttc aagatcgttc
    33901 gattattgat cagagagagg ttttatcgga tacattaagt tttcatcgtg gcttgcgttc
    33961 tgttttaaga caagatccag atgttattat ggttggagaa atgcgagatc ccgaaacgat
    34021 tcaaacagcg ataaccgctg ctgaaacagg gcatcttgtt ttttctactt tgcataccaa
    34081 ttctgcttca caaacgattg atcgcattat cgatagtttc cctgcttccc aacaatcgca
    34141 gattagaact caattagcgg caacactagt cggaattatt tctcaacgtt tagtaccgcg
    34201 attaaatggt ggtagaattc ccgcctgtga agtaatgctt accaactcag ctattcgtaa
    34261 tttaattcgt gaagataaaa tttatcaaat tgatttggtt attgaaacaa gcattcaaga
    34321 acgtatgatt tcattgaatc gttcattggc taacttggtt aaacaaagcg aaatttcttt
    34381 agaaactgcc gaatcgtatt cattaaaccc ggcagaatta gctaatcttt tagctcgtta
    34441 attaaactct atggcaaagt ttcgttatac aggcagaaat gatttaggag aaattcaaag
    34501 cggcgttgtt gaagcaggca gtcgtgattt ggctattaaa attttacaag ataataaaat
    34561 atatgttctt tctttagagt ctttagaaaa gaagggattt ttggataggc tgcttcagtc
    34621 atttcaacgc gtcaaaacaa aagaccttat gatttttact cgtcagttaa gtgttttact
    34681 ttcggcaaag gttcaattag ttgattcgtt gaaagctctt tataatcaag tgacagatcc
    34741 cttgttgaag gatgctattt ttgatgtttt tactgatgtg gaaggaggga tgtatttttc
    34801 tcaagcttta gcaaagcacc cgaaggtatt ttctgatttt tttgtaaaca tggttcgttc
    34861 tgctgaatta tccggtcgtc ttgaagagac gttgaattac cttgcggatt acttagaaaa
    34921 agataataat ttaaaaacaa aattaagaaa tgccatgatc tatcctgcat ttatcattgt
    34981 ggtgtttttg gccgtaatga tattggtagt cactgttgtt attcctcaat taaaaggaat
    35041 ttttacagaa tctggtagtc aattaccatt tattactaga gtgctgatga gcgttggtga
    35101 ttttttgctc aattggggcg tggcgattat tgtttttcta gttattgcta tttttctttt
    35161 cgtaagatat attagaacac cggaaggaaa ggctttttgg cagcaacttc tactttcgtt
    35221 gccgattatt ggaccaattc aaaaaaaggt agctattagt cgctttgctt cttcctgcag
    35281 cgcattgctg aaaggtggat tgccgatagc gagctcgttg gaaatttccg ggaaaattgt
    35341 tagcaatatc gtttatgaag aaattcttac caaaacagct gatgctattc gaagtggtgg
    35401 tactatttct aattttttag ctcaataccc agattatttt tcaccaatgg ttatccaaat
    35461 gatagcggta ggggaggttt caggtcaagt tgatgaattg atgcagaagg tagctgattt
    35521 ttacagtaac gaggttgatc agacagccgc gaacctagca gaaattattc agccagttct
    35581 tatcgttatt cttggtgttt ttattggtgt ttttgtggca gcagtgctaa tgccaattta
    35641 taacttagct caagtatttt aaatttttat agatattctt tataataaag gtcgagatta
    35701 attaaactca attttaaact caaaacatga ataaaaaaga aaaaggtttt accttaattg
    35761 aaatgttggt ggtagttgcg gtgattgggc ttttagcttc attgatttta gtaggtttgt
    35821 ctagttttag aaccagaggt cgagatacac gacgagtggc tgatacaaaa gaaattcaga
    35881 atggattgga gttgtattac atgaaaaatg gcagttatcc agtgctgacg tctggtaatt
    35941 gggacgattt agaacaagaa ttagtaaatg ctaacctagg gattactaat ttacctcagg
    36001 atccaacggc gcctaccagg tcttatgact atggttcgga tggccaacat tatgtgatcg
    36061 cggtaacgat tgaagatcca gatgctaata aatctctttt agacagcgat cttgatggtt
    36121 cgcaatatgg tggtgttaat tgtggtgatg cttcaaatga tacaatttat tgtataggaa
    36181 tgtaattcgt atttatatgc tacagccagt tattattgcg attgtttttt tactcgggtt
    36241 gggagtaggc agtttcatca atgtaattac cattcgcttc caacccgata aaaatatccc
    36301 ttttattaaa ttggtcagcg gtcgatcacg ctgtaatttt tgtaaacatc aactcaaatg
    36361 gtatgatctt ataccaattt ttagttttat ttttttaaaa gggaaatgtc gctattgtgg
    36421 taaaaaactt tcttggcaat atcctattat tgaaatattg acaggcataa tttttgccgg
    36481 agtaacctac cggcttttgc gacttgatgt tattcgttta cttctagcgt cgggcactat
    36541 acctttttgg atttggttag ttttccttct ctttttattt tacagcgctg ttttgattac
    36601 tctttctata attgatatca agcattatct tttaccggat aaattaattt attcagcaat
    36661 aggcgtttca gtagtggcag atttactttt ttatggattg agcaaaagca gtaaatttaa
    36721 tttcccatcg tgcggtttga attttttaag tagttacatg gattatttta attgccaatt
    36781 taatgtttta acctcttatt tatttggtgc attggcagta ggagggtttt tgtttttagt
    36841 ttatcttttt acacgaggaa aaggaatggg attaggagat gtcaaattag ggattttaat
    36901 aggtttaatg cttgggctga caaatggtat tttagctttg gtaatggcct ttattattgg
    36961 ttctattgtt ggtataatat tattagcctc taaaaagaag tctcttaagg actgggtgcc
    37021 atttgggcca tttttagcat taggagtgtt tattgttatt ttttggggct cattcttcgt
    37081 cgaatcatat ttttcaatat ttaactatct tatatgaaag aagttcctat ctcaaacact
    37141 ctatattggg cagccattat ttttatggca atagttttcg tagcgacaat tattttaaat
    37201 agctaccttt ttgttttgga taatcagtta actcaacaaa tttatttatc tcgagttgcc
    37261 ttacaaaaag ccactttaat tattgattac ggcaatggtc aaagaaaaat ttttgaagga
    37321 gatgttcctg cgggcggtct tagtttatat gatgttcttt tggcttcggc tcaaactggc
    37381 agtattagcg ttcaatttag caaaacagcc gatggtcgtg tttctcttgt taaaatcgat
    37441 cagtacgtaa acaacatgga tcattattgg gaattacaga taccaaaatt taattggaaa
    37501 agagctttga atgatagtac aattgattta acgcaatttt atcttgtagg cggtaatact
    37561 gcttatctcg tatataaata aaaactaaat taaaatggaa attatcaatg gggaaaatat
    37621 ttctcaagaa attcttgagg atttaaagcc gttttttcaa aaacacaaag cagcgttagc
    37681 tgttatttcg ataggcaata atttaggggg agaagttttt attaaacaaa aaaagaaagt
    37741 tgcagattat ttatcaattg aatttattca ttttcatttt aaagaagaca tttctaataa
    37801 attattaagt aagaaaatta atcaaattgt tagacaaaat tttattaaag gtgttgtttt
    37861 gcaattacca ttgcccaaaa aatttaatac ctatgctctg gtaaatatta ttccttctca
    37921 aaaagacgtc gatgtcttaa ataatgccca ctttgcttcg tattgttttc aacgtagttt
    37981 aattttacca ccggctgttg agacaataaa aataattttt gaaaaatata atattaacgt
    38041 tcaagagaaa aattgtggga ttgtaggagt tggacgatta gttggttttc ccactttgat
    38101 ttggttgtta caacagaagg ctacagtgtt tgcggtggat atttctacta aacagccaga
    38161 aaaaattttg gcgcaatgtg atataataat tacaggcaca ggtagccctc atctgattaa
    38221 taaaaaattt gttaaaaaag gggctattat tattgatttt gggttttcta aagaaaatgg
    38281 aaaaatcaaa ggtgatgtca atatagattc agttaaaaag aaggcgctat tagtaacgcc
    38341 aacgcctggt ggcactggtc ccattcttgt tgcggcatta tataagaatt taaaaacatt
    38401 actacaaagc aaacaaaaat aaggccgaat ttaacctata agattaaaaa attgtttatt
    38461 taacatatta tggaattagc agctaatcct gggaaaaaaa taataataga agtagatgga
    38521 ataaaatggg ctcgatatcc tataaaaacc cattttatta cggaaaaaga taatttagat
    38581 gaaattataa aaaaatacgt tttgcctaat gctcaaaaag gagatataat ttgccttggt
    38641 caaaaaattg tttctatttt acaaggttta attatttata aaaaagattt aaaagttgga
    38701 tggtgggcaa attttttgtc aaaatttgtt aaaaagacgc catatggttt ttcggtcggt
    38761 aacccgttaa aaatgcagat agctattaat ttagctggtc ttccgcggat tttatttgct
    38821 gctgttgttg ctggtatttc taaaatattt ggtttttcgg gcgtttttta tcgattagcc
    38881 ggacaccaaa tttcgcaaat tgatggtttt tatgccgaag cctttcctca atatggcgaa
    38941 atgggcatct tagggccaaa aaattgtaat gaactctgcg aaaatttaca gaaaaaatat
    39001 gaccactcct ttttggtaat tgatataaat gacttggggg gtaatatctt aggctgtagc
    39061 aaagatttag caggaaaaga gggtctaatt cttaaaattt taaaagataa tcctgctggg
    39121 cagtctaatc aacaaacacc tataataatt ttacgacaat tatgcaatta aaaaatatta
    39181 ttaatcacga aacaaaaagt gttaattccg ccgctattat tttggcaata gcgtctcttg
    39241 tttctgcttt gctgggtctg attagagatc gtcttttagc gggaactttt ggcgctggaa
    39301 gagaattaga tatttactac attgccttta aaattccgga ttttatcagc atagttttaa
    39361 taattggcgc aatttctgcg gcgatcactc ctatttttag tcattattta gttcgctcca
    39421 aaaaagaagc gtttgatttt ctctcaaatc ttataaacgt gtttttatta tttttaatta
    39481 tcctttcttt aattttaatt atttttgttc ctcaacttct ttatctgatt gcgcctggat
    39541 tttcggttga agaaagagaa atggcggctg ttttaactcg tattatgttt ttaagcccga
    39601 tattgttagg aataagcaat atcatatctt ctgttttaag agtttttcaa aggtttttag
    39661 ttacttctat agcacctatt ttgtacaatg tcggtattat tattggtatt ttgttttttg
    39721 tacctatagc cggattaaaa ggtttggctt ggggcgtagt gttgggagcg ttttttcacc
    39781 ttgtttttca aattccaacc cttatccaat taggatttaa atttcaaaaa aaattaaaac
    39841 taagagatcc aggtgtaaaa aaagcaatag agttaactat ccctcgttct attggattag
    39901 ccacaaccca aattaattta attattatta gcgctattgc ttctatttta agttccggca
    39961 gcatagcgat tatgaattta gcagataatc ttagtcggcc tctttacacc ttcatcgctg
    40021 tttcgttagc gacagctgct tttccagcat taaccctggc tttttctaaa caagaaaaaa
    40081 ataaatttca agatattttt tcagcaactt ttaaaaaaat tttttggcta acattttcat
    40141 taggcataat tctattcctt tttagagatt taattgtaaa aattattctt cacacaggaa
    40201 aattcgcact gacggatgtt cgcttaaccg ctatttgttt tggaatcttt gctgttggta
    40261 tatttgctca aggacttgta ttattaattg ctaaatcttt ttacgcgctc cacgacacta
    40321 agacgccaac cattattagt attttaagtg caatttttaa tattgtttta tgtttcgggt
    40381 ttattaaatt actttctttc cctaatgcct ttagtcaatt ttttcttaat ttattaaata
    40441 ttaatagtct tcaactaacg tcagtggcag ctcatccatt cattgttaaa tttttaggaa
    40501 tttataattt aaccggcata gaagtcatag ctttgccttt ggctatttct gtttccgcgg
    40561 tttttcaatt tatattcctt tatattcttt tttgtcgtaa aaaagagaaa gtatttcaag
    40621 aacaatcact tagtgaaata aattaatgaa aaacattcgt aattttgcca tcattgctca
    40681 tattgatcat ggaaagtcaa ctttggctga tcgttttttg gagttaaccg gcgcaattga
    40741 taaaagaaaa atgaaagatc agtttttaga ccgtatggat ttagaaagag aacgaggaat
    40801 aactattaaa atgcagccag ttcggatgat ttatcgttct agaacaaatt ccgaatatat
    40861 tcttaattta attgataccc ccggtcatgt agattttagt tatgaggttt ctcgaagtct
    40921 ggcggcggtg gaaggagcga ttcttttagt tgatgcaacg cagggtattc aagcgcaaac
    40981 aatgggcaat tatcaattag ctaaaaaaca agaactcact attattccag tcattaataa
    41041 gatagattta cctaatgccc aaattgaaga ggtagaaaaa caaattatgg atttatgcgg
    41101 tattagccaa aaagaaattt ataaggtatc agctaaaaca ggcgaagggg ttgaagaatt
    41161 gcttcaggca gttattgaaa aaattccttc accaactgaa tctgacaaaa agcctttacg
    41221 agcgcttgtt ttcgattcca tctacgataa tcatagaggc gttatttctt atgtacgtat
    41281 tgttgatgga gaagttagag gcggtgagac gggttttttg atggcttcgg aaactctttt
    41341 taaagttgct gaggtgggaa tttttaggcc ttggttagag aaaacagaaa ttctttcgag
    41401 tggggaaata ggatatattg tgacaggact aaaagaacct gtgaaggtga gagtaggaga
    41461 tacaataaca aaatttaaaa aatcttctcc tagttatagt agtgcaaatt cgaaaaataa
    41521 aaatgataaa ataattaaag aaacgggaaa aaataagtac ttatcaattg ccggagtaga
    41581 aagattgccg gggtataaag agccagaacc aatggttttt gcaagcgttt atccaacatc
    41641 aaatgaaaac ataacgaccc tcaaaaatgc tattcagaaa ttaaaactta atgatcctag
    41701 tttggtttat aaaacagaat tcttttctgc tttgggtcaa ggattttgtt tgggtttttt
    41761 gggattgtta catttggaaa ttgttaaaga acgtttggaa agagaatata aagtagatgt
    41821 tatttttact acaccctcag tcaattataa agtagtaatg aaaaatggat tagttaaaga
    41881 tgtttttcgg gcttcggatt ttccagttcg cgatcaaatc gaaaaaattt tagaaccgtg
    41941 ggttcgcttg gaaattatta cgccgttgaa ttttgttgga gccgtaatgc aattgaaaga
    42001 tcggcaccgt ttaaattatt taaccactca atatttagga acactgataa ttttagtttt
    42061 cgaagcccca ttaagcgaaa tcattattaa tttttacgac aaattaaaaa gcgcaacgga
    42121 aggatatgcc tcaatgagtt atgaattttt agaatatcgc gaaggagatc ttgttaaatt
    42181 agagattttg ttagctggcg ataaagaaga aagtttggca aaaattgttc accgccgttt
    42241 tttagaagaa gaagcgagaa aaatggttga aaaattaaga agtgttttac cgcgcgaaaa
    42301 ttttgctgtt gcaattcagg cagtagctga aaatcgaatt attgctcggg agactttgcc
    42361 tgctttaaaa aaagatgtga ccggttatct ttatggaggc gatcgcactc gaaaaatgaa
    42421 attatggaaa aaacaaaaag agggaaaaaa gcgattgaag gaacgaggaa aaatacgtct
    42481 ttcaactaaa gcttatttgg atattttaaa aatggaataa tgttcaagat aaatttttta
    42541 ttcagcatta attagaaaag caattaatga aaattaaagc gacaagtaag attggttcgg
    42601 aataattttt cttgtttttt tattagaact tattaaaata taatagaaca tggcttggag
    42661 atccaaaatc cttttggttg ttttatttat agtggcatct tttacactaa tttattttta
    42721 tcatcaatta acacttgaaa aaaaacttca agctgactac gaagcaattg ttcagcgcgt
    42781 aaatgaactt caaaaagaaa atgctttatt agaagatgaa ttggcaaaaa tggaaaatga
    42841 ggaagagcta gaacgattgg ctcgcgaatt atatgcgtta aaaaaacccg gggaaaaagc
    42901 gatgattatt ccccaagaag tgatggaaaa attaattgag gatgatgcga ttcaaaatgt
    42961 ttccccgcaa gcaccttcca aaatttctca atggtttcaa gaaataaaac attttttcgg
    43021 taatttattc taattaatga ttaataaatg ttggcagtga aatgcgagag tagcacagtg
    43081 gcagtgcgct tgcttcccaa gcaagatacg cgggttcgat tcccgtctct cgctcctcag
    43141 ctgagagaaa tgttctgctg aaattctttg ccgatgtagc tcaattggca gagcagtccc
    43201 ttcgtaaggg aaaggttgtc ggttcgactc cgaccatcgg ctctttaaac aaattcacta
    43261 aactaaaaat tttatgaatg atttaaaatc gaaaattcaa gaaattcgca ataatattaa
    43321 aaaattggaa aaagagattg atttagaaaa aaagaaatta gagctggaag aattgaataa
    43381 aaaaatgagc cggccagatt tttggaaagt accggaagaa acagttgata ttttaaagag
    43441 agtcaaagaa ttaaaaggtg agattaattt ttggatggaa ctagagaaaa aattagatga
    43501 agtcgaagat ttatctgaat caattgattc agataaatct tcggccgacc ttgaaaatca
    43561 actgataaat ttagaaaagc aaattgaaga taaaaaactt gaaatttttc tttccgaccc
    43621 ttatgacaaa aatgaggcca ttatgatttt aacagctggc caaggcggac gcgatgcgga
    43681 agatttttgt cgaatgcttt ttaggatgta tagtaattat tttgcaaaga aaaaatggcc
    43741 ttatcaaata ttatatcagc atttttcaga agagccgggt ggttctaagg atgcgggagg
    43801 agaaatgggt ttgaagaata tttccttaga aatcaacacg ccttatgctt atggtttttt
    43861 gaaaaatgag tcaggcgttc atcgattagt tagaatttct ccttttgatg caaaaaaaca
    43921 gcgtcacact tcttttgttt tagtggaagt tttgccgcaa atagaagaaa ttgatttaaa
    43981 agacattgaa ttaagagatg aagatttaaa aatagaattt atgcgttcat caggtccagg
    44041 tggtcagaat gttaataaaa gagaaacggc tgtgagaata gtgcatttgc ctacaggatt
    44101 aatggccgtt tgtcaatcag agcgttcaca gcagcaaaat agagaaaaag cgcttcatct
    44161 tctaaaattg aaaattttta acgctttaca gcaacaaaaa gaaacagaac gaaagaattt
    44221 acgaaaacgc atagagccat cctggggaaa tcaaattcgc aattatgttt tgcagcctta
    44281 taaattagtg aaggatttac gaactaatgt tgaaacttct aatatagacg ctgttcttga
    44341 tggcgaaata gataatttta taaaagcaga aatgttatta aatttaaacc cttcttctaa
    44401 gagaaaatag aaaattttag atttctctgt ttacggatgg acgactgagt aagcaaagtt
    44461 tagatttgcg cgtttaattt taaatattta ttgttttttg atttgcaaaa atattcttga
    44521 agtgctaaaa taaatattaa tatgaatgaa gagttggttt attttcaaaa tgtgacaaaa
    44581 gtgtataata gtcatacagt agcccttgat aatatcaata tcaagataat gcgagaagag
    44641 tttgtggcta ttgtgggaaa atcgggcgct ggaaaatcga cgcttctcaa attgttaatt
    44701 ggcgaagaga agcctacaaa aggaagaata ttttttgaaa aacaagatgt ttctcgttta
    44761 tcatataaag aattacctca gttgcgcagg cagataggta tgatttttca ggactttaag
    44821 cttttaccag ataaaactgc ttttgaaaat gtagcttttg cgttggaagt agctggttat
    44881 cgtcctaaag aaattaaaga aaccgttcct cagatgctcg aactcgtagg attaaagaat
    44941 aaaattttta attttcctca tgaattatcg ggtggagaaa aacagcgagt agctattgct
    45001 cgtgcgctta tcaacaaacc tgacctttta attgccgacg agccaacagg caatttagat
    45061 cctattaata ctgccgaaat tattaaactt ttacttaaaa ttaatgaatt aaaaacaacg
    45121 gttattttgg caacgcataa taaaaatgta gtggatatgc tgaatacgcg agtgatttct
    45181 ttagataatg gtcgagttat tcgtgatgag gaagaaggtc aatttattat ttaaagatct
    45241 atgtttgtat ttttagcgag gattatcaag tatggttttc aaagtttttg gcgcaatggt
    45301 ttattgtcta gtgcgacagt agccattatg accttaacgc ttcttttgtg ttctggatta
    45361 atcatttttg gcaatgccgg aaatgttgtt ttgcaaagtc tagaaagtaa aattgacgtt
    45421 agcgtttatt ttaaaactaa tgttggtgaa aatcagattt tagccattaa aaaaaatatt
    45481 gaacaattag atgaggtggc tagtgtcgaa tacatatctc aagaagatgc tcttgcgact
    45541 tttaaagaaa gacataaaga cgatcccgtt atcattcaat ctttagaaga gctcggttat
    45601 aatcctttat ctgcctcttt aaacattaga gctaaaaatc cacgcaatta cgagggtatt
    45661 gcaaattact tggaaaaaga aattccttct gaatttattg ataaaattaa ttatagtcag
    45721 aatcagatta ttattgatcg tttaaacaaa attataagcg cttttcaaac aggtggattg
    45781 attgcagttg gcgttttggc tttggttgct tttttgatca cttttaatac aattcgtttg
    45841 gctatttatt ctaacagaga atccataggt attatgcgat tagtaggtgc ttcgggttcg
    45901 ctaatccgag ggccttatgt agtagaagct ttcattattg cgttagcttc tacaatatta
    45961 tcttcgattt tacttattcc tattttgtat tctattgcgc cttactttaa taattttatt
    46021 cctgaattta atattattga atatttctgg agtcatttct tttatctttt tggtattcaa
    46081 tttcttgttg gcttggcttt ggctattctt tctagtgtta ttgctattag aaaatatctg
    46141 aaagtataga gcaatatcag aagttgaata tttctaattg agaggataag agattaaaat
    46201 aaaaatataa aatatgccat tgttattaga aatagtctaa tggaaaacac aagacttttt
    46261 taaatcttgt ccgctctttt aaacttcaac tttaaatttt gatttttgcg gggtcgtcta
    46321 atggtaggac acggcgctct ggacgccggt atcttggttc gaatccaagc cccgcagctt
    46381 tggaaagaac gtgaagaaaa tcggagaatt acttagtttg gccgaaaaga aaaaattgat
    46441 gtatatactc aaaattttag cgataaaatc ttacactttt tgtttgcgct gaggaatttt
    46501 atttttataa taatatttag atgagtaaga cggttaaaat tttaatttta tcggacttta
    46561 ttattatttc agccttcggt tttttaaatc ctatttttgc gctttttctt actgataaaa
    46621 ttgctggcgg aacaattgag actgttggtt atgcaacagc tttttattgg gtagcagctg
    46681 tgctggtgcg tttaccattg gctcgacgtg tagatagtac taaatccgaa aaggacgatt
    46741 tctattttat gattcttggt tctatcctaa tttcattagt tccatttttg tatatattta
    46801 gtactcaaat ctggcatatt tatcttattc aggctcttta tggattaggg tattctttgc
    46861 gattacccgg ttggtatgca atgtttagtc gacatatgcg aaaaggaaga gagggatacg
    46921 aatggagtgt taatagtatg gtagcgggac tgggttcagc aattaccgca gcgctgggtg
    46981 gtgtattggt gggacgactg ggatttgatg tgctttttct ttttattgga ctgctgagcg
    47041 tttttggttc tattgttttg gtttttttgt attacatatt agagccaaat caccatgttt
    47101 aatttaaatt aaggttatca atagaaaatg catgtttaga ttgaggcgaa aagtagttga
    47161 atttaaaaca tattcatttt cattgttttt attctattcg gagaattaaa ttcgcggact
    47221 ttagaaggaa atagggaatt aataaaaaat tagccatttt ttatttgctt tagtttttgg
    47281 aattacagat taattatagt ataatttatt tagtcgataa aaaatattga gtatgaagga
    47341 ttttttacaa aaaattacta tagatggtag ggcgatgtat ttggcttatg atcatggttt
    47401 tgagcatgga ccaatagacc ttcccaacca atcaataaac ccagattata ttttaaatat
    47461 tgctgttgag ggtggttata atgcgattat tcttcaaagg gggttagctg aaaaatatta
    47521 taccggtact aaatatcaaa aacagattcc tttaatttta aaaattaatg ggaaagctaa
    47581 tatctggcaa ggcggtgacg tttattctta tcaaaattgt tctgttaaat atgcaaaaac
    47641 gttaggagcg agcgcagtgg gttatactat ttatttgggt tctgctcacg atggaaaaat
    47701 gttttctgat tttggaaaga tagttgaaga ggcgcatcaa ctaaacatgg cagcaattgc
    47761 ttgggtttat cctcgcggcg aatttgttaa agatgaagtt tcaaaagaaa ttacggctta
    47821 tgctgcccga ataggtttag agttaggagc ggatatggtg aaaataaaat attctggttc
    47881 caaagaaaat tttgcatatg cagtgaaggc ggctggcaaa acaaaagtag ttttatctgg
    47941 cggtcctaaa gtgaatgatg aggaattttt acaaataaca aaagacgtga tggcagcagg
    48001 tgctagcggt gtagcggttg gaagaaatgt gtggcagcat agtgaacctc taaaaataac
    48061 gaagaaaata aaagagataa tttttagtta gttaatccac attttaagtt agcaagacca
    48121 cttgacttta tttaaaattg gaataaaatt tctataaatt gagaaataaa aaggctttgt
    48181 catattttta ttactcaata gtcgattaaa ataattttta aaaaaatggc agaaaaaaag
    48241 aaatttgaac gaaaaaagcc acacgttaat gtaggaacca ttggtcatat tgaccatgga
    48301 aaaaccactt taacggcggc tattttacac gttttaagtt ttaagggttt ggttgaaaaa
    48361 gtcgaaggcg ttgatcagat tgataatgct ccggaagaaa aagcacgagg cattacaatt
    48421 gctttgcatc actcagaata cgagtcagaa aaaagacatt atgcacatat tgatgcgcca
    48481 gggcatgctg attatatcaa aaacatgatt acaggtgcag ctcagatgga tggtgcaatt
    48541 ttggtggttt cagccgctga tggtccaatg cctcaaacgc gagagcatat tcttttagcg
    48601 cgacaagttg gcgtaccggc tattatagtt tttcttaaca agattgatca agtaagcgac
    48661 ccggaattaa ttgatttggt tgaaaacgaa gttcgagaac ttttaacaaa atatcaattt
    48721 ccaggtaatg aagttccggt tattcgtgga tcagcgttgg cagcacttaa tgccaaatca
    48781 ttggatgatc cagcctgtaa gcccatttta gatttaattg aggcaatgga taattatatt
    48841 ccacaaccag ttcgagaaat tgataaacca ttcttaatgc ctattgagga tatattttct
    48901 attgaggggc gtggaacagt tgtaacagga agagttgaaa gaggtatttt gaaaacaaat
    48961 gaagaggttg aagtggttgg tttaaggccg acaattaaaa cggtagttac cggtattgaa
    49021 atgtttaata aaacattaga cgaagctcaa gcgggtgata atgtaggaat tttattgcgt
    49081 ggtttgaaaa aagaagatgt agaaagggga caagtaattg ctaaaccagg aacagtgacg
    49141 ccgcatacag aatttgaagc agaagcttat attctcacca aagaagaagg aggtcggcat
    49201 accccatttt ttaccggtta taaacctcaa ttttatattc ggactactga tgtaacaggc
    49261 gaggttactt tacctcaagg aattgaaatg gtaatgccgg gcgataatgc tcatttgatt
    49321 gtaaaattaa ttgcaccggt ggcattggaa gaacaacagc gttttgctat tcgcgagggt
    49381 ggcagaactg taggcgcggg cgttgtttca aaaattatta aataactagc tcttttggaa
    49441 ctgcaatgtc taaatcaaat gaaaaccaac agaaaataag aattaagtta aaagcttatg
    49501 atcataaggt gctagataat tcttgtaaac aaatttttga aacagctaat cgttacgtga
    49561 aagatattat tgggcctatt cccttgccga cagaaatcaa acgctatact gttaatcgtt
    49621 cgacgttcat tgataaaaat tctcgagagc aatttgaaat tcgtattcat aaaagattga
    49681 tagatatcct caaccccaat cagaaactta ttgatgcctt gatgaatctt aatttgccgg
    49741 cgggggtaga tattgaaatt aagatgtagg tttttggagg aaacaaataa atgccgggta
    49801 tgctctttag agccagcaat gattctaaag agttcctccc cggcatttat ttatctaaaa
    49861 tattgataaa aaataattta tttttaaaat atcagacaat atgactaaat ttatactggc
    49921 tcaaaaaata ggaatgactc aaatttatca agatgataaa gcaattcccg taaccgcact
    49981 ggaagccgga ccagttgttg tttctcaaat taaaaagcaa gacagagatg gttattatgc
    50041 tattcaagta ggatttggaa aaaaaagaaa tctatcaaaa gcagtgagaa atcatctaaa
    50101 aggattagga gatctgcgct ggttgaaaga atgtaaaata acaaaagaag aggcagatgc
    50161 ttttaaacga ggcgatagaa tcgatgtctc tgtttttaag gaaggagaaa aggtgaaaat
    50221 tattggcacg agtaaagcta agggatttca gggcgttgtc aaaagacacg gatttcatgg
    50281 tggccccaaa agtcatgggc aaaaagatcg tttacgagcg ccgggatcca ttggcgcaac
    50341 tcaccctcaa catgtattaa aagggaaaaa aatggccggt cgaatgggtg gcaatcggat
    50401 aactttgaaa aatgttaaga ttgttaaaat tataccggaa aaaaatatta tttgtgtaaa
    50461 aggagcgatt cctggaaaaa gaggcgaatt agtccaaatt ttatcagaat aaagtatgaa
    50521 gactgatctt tataatcaaa aaggagaagt gataggtcag attgcattat ctgataaaat
    50581 ttttggagca aaatggaatc cagttttggt gcatcaggtt ttagttgcta ttaaaagtaa
    50641 tcagaggaaa cccatagctc atgctaaaga tcgaggagag gtgagtggtg gcggaagaaa
    50701 gccttggcga caaaaaggaa caggtcgggc tcgtcacgga tcaattcgct cgccattatg
    50761 gattggcgga ggtgttacct ttgggccaac gaaagaaaag aattatcaga aaaaaattaa
    50821 taaaaaagcc aaacagcaag cctttatttc tgcactgtct aaaaaaatga ctgatggaga
    50881 aataaaagtg gtagaaacga ttcacctcga acattccaaa acaaaagaaa tggcaaaaat
    50941 tttatcttca tttttaaata ctaaaaaaag aaatcagggt cttattgttt taccgtcgag
    51001 tgacccatca attattcgag caacgcgtaa tttatctttt gccgctgcca caattgctga
    51061 taaccttaat ttgcctgata ttcttaaata taaaaacatt atttttacca aggaagcaat
    51121 agaaaaatta aatgaaaaaa taaaataaat ttatgagcat tttttcaaaa aaacaatcta
    51181 aaaaggaaga atctgaagat caagacacat tgaaggaaga aaaaaaattg aaagaagaaa
    51241 aaaaagagac agagaagcca tcacttaaaa aagaaggcag aggggttata tttaataaaa
    51301 atattttatt aataaaagag cctaaagtaa cagaaaaaag ccaatcgatc caagcatatc
    51361 ggcagtatgt ttttaatgtt gtaagacaag caaataaaaa tgaagtaaaa aaagcgattg
    51421 aacggattta taatgttact gtaaaaaaag tgagaatgat taatataccg gccaaaaaaa
    51481 gaaggtcggg tcgttctgta ggtaaaaaat caggttacaa aaaagcgatt gttaccctca
    51541 aagaaggtca gcaaattgag attatgtctc agaaataata acatgaaaaa gcataaacca
    51601 actacaccat cacgaagaca tatgacagct gttgaatata aagagattgt gacgacaagc
    51661 gagccgttaa aaaaattgac ggctcctttg aaacagcaag ctggccgtaa ttcttttggt
    51721 cgaattacag tgagacatag agggggaggc aataaacgat tgtatcgact agttgatttt
    51781 aaacaagata agtttaatat aaaaggaaca attaaagaga tcgaatatga tcctaatcgc
    51841 aacacttata ttggtcgtgt agcgtacgaa gatggcgaat atcgatatgt tcttttgcct
    51901 gagggtatga aaacaggaga tcaaattatt acaacagaat taacatctat tactccgggt
    51961 aatcgattac ctttaaaaaa cattcctgtt ggtacgagag tttttaacat tgaacttttg
    52021 cctaaatctg gaggtaaaat agctcgaagc gcaggctcag cggctgaagt gatggggata
    52081 gaagataaat attgcattct caaaatgcca tctggtgaaa ttagaagagt actagccaat
    52141 tgttatgcaa ctatcggttt gccgtctaat tcagaatatc gaactattaa cattggcaag
    52201 gcaggaagaa cgcggtggtt gagtgtacgt ccaacggtta gaggaacagc tatgaatcct
    52261 tgtgatcatc cttatggagg aggtgaggga cgtcaaccac gtggcacaaa aagaccgaag
    52321 agtgcttggg gtaaagtaac cggtggtcat aagacacgca ataaaaagaa atggtcgaat
    52381 agtttgatta taaaacgaag aaaataaaat gagcagaagt cttaaaaaag gaccatttat
    52441 taatccaaaa ctactgaaaa aaatggcaaa aattaagcct gattctggtc aggttattaa
    52501 aacttggtct cgatcttcta ccatttctcc cgagatgatt ggttatacgt ttgctgttca
    52561 taatgggaaa gatttcattg aagtgaaagt aacagaagaa atggtgggtc atcgattagg
    52621 tgaatttgcg ccaacgcgga aatttattaa acatggagga aaaatgcagc gagaattaga
    52681 aaagaaggcg gtcgttaatg aaccagcaaa ataaaatctc cttatggcta aagaaaacgt
    52741 tgcaattgca aaattgaaat atcttcatat ggcaccaaga aaagtgcgct tggtagccaa
    52801 cagcattaaa ggcaagcatg ttatagaagc tgaggctcaa ttagtatatg ctccgcaacg
    52861 agcggcaaag cccatgctta aattattacg ttcagcagtg aataatgcat caaacaagaa
    52921 gttagacaaa aataagcttt ttgtggcatc gattacagtt gatgaagggc caatgctaaa
    52981 aagatggttg ccgcgggcaa tgggaagagc tacgcccatt cagaaaaaaa tgtcgcacat
    53041 cacccttgta ttagaagaaa aaggagaatt caatcgttat aaagttcctc aagcaaaaat
    53101 tactaaatca gaaaaaaaat taaccgaaac caaaggagga gtaaaagaaa aagagcacga
    53161 attaaagaaa aaatctccaa cggtcgagga gacaaaaaag aaacctcaag aaggatttgc
    53221 tcgcaaaatt tttagacgaa aagaaattgc ttaaaataga caaaaaatat gggacaaaaa
    53281 attcatccaa aatcttttag attaggaatt aataaaaatt ggagtgaacg atggttggct
    53341 tcaaagaaaa cacgtccgat tattttggaa gaggatgtta aaattcgtaa ttttctcaat
    53401 aagaaattaa ggaatgcctt gattgctgac gttgaaattg aacgattaga gaataatatc
    53461 aatcttatta ttaaaacagc ccgccctggt gttattattg ggagaggcgg taagggaatt
    53521 gaagatttaa agaaggaagt tgaaaaaatt attagacgtc atcgcaaaga aagaaaatat
    53581 cctcttaatt atgttctaaa tatttcaatt gaagaagtca aaaatcctac agctaacgca
    53641 gcaattgttg ctcaatctat tgctgcctca attgaaaaga gaatgcctta tcgacgatta
    53701 atgaagaaag caattgaaga tgccaagcaa aataaagaag taaaaggaat taagattaaa
    53761 ttaagtggac gtttgaatgg tgtggaaatt gctcgctctg agtggttgga ttggggagca
    53821 atgcctttac aaactattcg agctgatatt gattatagtg aagctactgc attttgtact
    53881 tacggcacta ttggaataaa ggtgtggatt tataaaggag aaatttttga taaaaacaaa
    53941 aacaaagagg gaatatcaga aatgtctaat aaacaaacaa aataaatatg ttgatgccta
    54001 aaaaattaaa acatcgaaaa tatcagaaag ggcgatctcg caaaagatca atagaaactc
    54061 gaggaacatt tttgactttt ggctcatatg gattgcaagc gcttgataat atatggttaa
    54121 cagctcaaca aattgaagca ggaagaaagg caattgtagg ttatttaaaa aaaggaggta
    54181 aagtttggtt gcgtgttttt cccgataagc ctattactaa aaaaccgccg gaagtaacaa
    54241 tgggaggtgg aaagggggat ttggatcatt ttgcatttcc cgttcgaaaa gggcgaatta
    54301 tttatgagat tgatggtgtt agtgaagaaa ttgccactga agctttaaga agggcaggaa
    54361 ctaaaatgcc cattaaaact agaatcgtca aaaaagaata gaaacacttt gttatggaat
    54421 tcaaagaatt aaaaaattta cctaaatcgg aattagaaaa attattagcg caaaacagag
    54481 ataaaataaa agaattgcgt tttaatttag cttttggtaa agtgaaagat attagcgaaa
    54541 ttaaaaagac aaaaaagagt atcgctcaaa ttttaacatt attaaatagt caaaaaaatg
    54601 aatcaaaaaa atcatccaat taaaaaaaga ttagaaggaa tagttatttc ggacaagatg
    54661 gataaaaccg ttgtggttaa ggtagaacgt tttaaaaagc atccctttta tcacaaaatt
    54721 atgacggttt caaaacattt taaggcccat aatgaaaata acgaagcaaa agaaggcgat
    54781 tatgtaatta ttgaggaaac gcggccactt tccagagaaa aaagatggcg cgtagttcag
    54841 attgttaaaa aaggaaactc aaaaaatcta gtcggtagtg ataaaattaa tgagtagaaa
    54901 tagaatatga ttcaaccgag aacaatgcta aaagtagcgg ataatagtgg cgctaaaata
    54961 gtgcaatgtt ttaaagtttt gggaggaact cgtcggcgct atgctcagat tggtgatata
    55021 attattgcat ccgttaaaat ggctcaacca agaaagagtg tttccaaaaa agacatagtt
    55081 cgttgtttgg ttgttagaca gaaaaaaccc tatcgtcgtg aagatggctc atatattcgt
    55141 tttgatgaca atgccgtagt tttaattgat aataaaaatc agccgcgggg tacaagaatt
    55201 tttggcccaa tcccgagaga aataaaacaa aagggtttcg aagaggtcgc ttcactagca
    55261 gtagagattt tgtaattatg aaaaagaata ataaaatgaa aattaaaaag ggagatgaag
    55321 tggttgtttt agtcgggaaa gataagggga aaagaggtaa ggtcatcgta gttttaccta
    55381 aaaataataa agcaatagtg gaaggcttga atttagtcaa aaaacaccgt cgaccaaagc
    55441 gccaaggcga aaaaggagag attatttcat taccccggcc aatagatgtt tctaagttag
    55501 cgttatggtg ttctcgatgc aatcgtcaaa caagagtcgg ttatcgtttt gaagacgata
    55561 aaaaattaag aatttgtaaa agatgtcagc aattgatcta aaaactaaat atcagaaaga
    55621 agtgaggcca aagttacaga aagaatttag gttaaaaaat gtgtatgctg tgccctctat
    55681 aaaaaaggtg gtaattaatg taggaatagg taaaatgtta agtacagctg ctcaatcaaa
    55741 aaacatcgat gattttctta aacaaatcaa acaagatgta agtttaattg ctggtcaatg
    55801 gccaaaagaa tcaagggcaa aaaaatcggt ctcatctttt aaaattagaa aaggagatgt
    55861 ggtgggattg actgttactt tgcggggtca gaggatgtat gattttttag cacgattagt
    55921 aaatattgcg ttaccacgca ctagagattt tcggggaatt tctattaaaa atttggacga
    55981 gcatggtaat ttaaatatcg gcattgcaga agcttcaatt tttccagaag ttaccaataa
    56041 tgttttgaaa catccttttg gtttagaggt aactatagta gtaaacgata aaaacaaaga
    56101 aagagtcgta aacatgttaa aatcgttagg ttttcctatt aaataatatg gccaaaaaat
    56161 cagttgttgc tagagcaaaa aaaccaccaa aatattcaac tcggatagtg aggcgttgtt
    56221 ttcgttgtgg tcgaaaaaga ggatatctca gagattttgg gttatgtcgt atttgcgtgc
    56281 gtgaattatc acggaaaggt gaaattccgg gaatgaaaaa agcgtcttgg taaataaaat
    56341 aagaatatta tggatactat ctcagatttt cttaatcaaa taaaaaatgc tttagcggct
    56401 aagaaaccgt ttcttttaac accccattct aaaatgaaag aagagattgc aaaaatttta
    56461 ttagaagaac aatatattag caattacaag gttagtggca aggcgccgaa gaaaatttta
    56521 agaatagacc tcaaatataa tgaagacgga acaccggcca ttcgagaaat aaaacgtatt
    56581 agcaaaccag gttgtcgtcg ctttataaaa acaaaaaata ttaaaagagt aaagtctggc
    56641 tatggtcgtt acattatttc cactcctaat gggttaatga caaatattga agctcaaaaa
    56701 catcatttgg gaggagaaat aatttgtgaa atattttaat attatgtcta aaataggaaa
    56761 gaaacttatt gtaataccag acaatgtaaa aatagaagtt ttagaaaatg aagtgatgat
    56821 taaaggcccg aagggagaat taaaagttcc tttattccct gattttaatg tagaacttga
    56881 aggaaattat ttaaaaatta ttcctacgcg tgagaagaaa tttacttctg cattttgggg
    56941 aacgcttcga tctttaatcg ccaatgcagt aaaaggtgta agtgaagggt tcgaaaaaaa
    57001 attagaaatt gaaggcgttg gctatcgagc taacgttgag gggaatattc ttgttttaaa
    57061 tttgggattt tctcatccga ttcgtttcga aatccccaag ggcatagata ttgtggtagc
    57121 aaaaaattta attactattt ctggagcaga caaacaatta gtaggtcaaa cagctgctaa
    57181 aatacgatct tttcgaaaac ccgaaccata taaaggcaaa ggaattcatt atgtcggtga
    57241 acacattaga cgtaaagctg gtaaaaaagc aacggctacg gcttaaattt aaaataattc
    57301 aaaaattatg aagcagaaaa gaacaaaaaa agaaaaacga ataagacgac acaaaaaaat
    57361 tcgagccaat atatcaggga caaaaacaaa acctcgaatt agcgttttta tttccaataa
    57421 acatatttat gttcaggcga ttgatgatga aaaaggcgta actttaacaa gcgtttcgga
    57481 tcaagattta aaaaagaaaa gcaaaaaatt agaattaagc aaagaagttg gattgacatt
    57541 ggcagaaaag ttaaagaaat taaatattaa aagtgctgtt tttgaccgag gtggttttca
    57601 gtttcacggt cgagttaaaa atatagccga tggcttaaaa gaaaggggaa ttaaatgtta
    57661 aatgtatgat taaaaataac tctactatta ataaaaagaa agagagtgtc gtagaattaa
    57721 aaaatcaagg ttacgaagat aaattacttg atttgcgtcg ggtaacgcgc gttgtggcgg
    57781 gagggaaacg atttagtttt cgagcaacag tggttgttgg tgatcgtgca ggaaaggtag
    57841 gcgttggtgt ggccaaagga aaagatgtta gtgaggcact cgctaaagca aaaaccaaag
    57901 ctcaaaaaag aatggttcaa gttaccattg ttaacgatac aattccgcac gaagtaaaag
    57961 cgaaatattg tgcagcagaa gtggttttaa aacctgctcg gaaaggtcat ggaattgtgg
    58021 cgggtggggc agttcgggct gtatgcgaat tagcaggcat caaagattta agtgcgaaaa
    58081 tattaagtcg taccccgaat aaattaagta atgctagggc taccattaat gctttacaaa
    58141 aaattaaacc caaaagagtt aatcaaagtg atacatcaaa atcacagaat caacagctgg
    58201 caaagtcaga agatcaagcg tcggaagttt taaaaaccga aaattaatta tgcaattaca
    58261 cgaattaaaa aaagaatata aaactaaaaa agcaaaaaga gtgggacgcg gaggtaaacg
    58321 cggaacttat tctggcaggg gttccaaagg gcaaaaagca agggcaggtg ctaaaattag
    58381 accagcagtt tatgatctta ttattaaaat tcctaaaaag cgaggggtta aacataaatc
    58441 aataacagaa aaaccagttc tgattaattt cgatattctt gaaaaacatt atcaagaagg
    58501 agacataatt tctcctcaaa cgttatttga aaagggtttg attaagaaaa gaggaggagt
    58561 attgccaaag gttaaaattt taggccgagg taatttaaca aaaaagttga tttttaaaaa
    58621 atgccaattt tctcagcaag cgttggccaa aattaaaaaa taatcattat gtctattttg
    58681 tctaaggttg cactgttatt taaaattaaa gatctcagaa aaaaaatttt gaccgtgatt
    58741 tttatcttgg tgatttttcg attatttgca acattgccca ttccagatat tgatactgct
    58801 cgattaaacg ccatttttgc ttctaatcaa cttttgggta ttttaaatat tttttcagga
    58861 ggaacacttc agcaactttc tattatgatg ttgggcgttg gtccttatat tactgcgacg
    58921 attattatgc agcttttgac gatggtgata ccgcgattaa aagaaattta ttatgaagaa
    58981 ggagaaaagg gaaggatgaa atttaatcag tggtctcgtt atataacagt accgctttgt
    59041 atgcttcagg cgtatgcttt tttgaattta ttaatttctc aagatgtcct tcctcattta
    59101 tccaccctga gccttatcag aaacgtttta gtggttacgg ccggctcaat gcttttaatg
    59161 tggttaggtg aattaattag cgaatacaaa gtaggtaatg gcatctcttt aattattttt
    59221 gctggtattg ttagtggatg gccggcaagt ttgcgttcgg ctattttatc ttatactcct
    59281 tcaatgctgc ctacctattt aatgtttctc gttgtagcca tcattgttat tttaggagtt
    59341 gtttatatta atgaagcaga aaggaaaatt ccgattgctt atgccaaaag agtaaggggt
    59401 aataaaatgt atggtggcgt ttctagttat ttgccgttaa aagttaacca agccggtgtc
    59461 attcctatta ttttcgctat ttctattttg ctttttcctc aatttattgg acaaattctt
    59521 gttgctatta aaacttcttg ggcggcaagt gcaggaaatt ttttaatgaa tatgcttaat
    59581 aaccagttgg tatattctat tttatatttt ttattagtag tagtgtttac ttatttttat
    59641 acgatggtaa cgtttgatcc aaaagaaatt tctacaaact tacaaagaaa tggtggattt
    59701 attccgggta ttaggccggg gcagactaca gctgattata ttagttttgt tttgcataga
    59761 attacttttg tgggtgcgct atttttaggc gttatagcta ttttacctaa tattttgcag
    59821 ggagtcacag ggacacaatt tttaactatc ggcggtactt caattttaat tgtagtttcg
    59881 gtagctatcg aaaccattaa acaggtagaa tctcaattag tgatgcgtga atatgaaggg
    59941 ttttaattta tattttttat aaatatgaag aaagccaaaa tccttatttt ttatggccct
    60001 ccaggcgccg gaaaagacac gcaagctcga cgtattgcgt ttaaatcagg agctgttatt
    60061 gttagctcat caaaattaat tgaagagaga atttttgatc ctcaactaca aaacgatcca
    60121 attattcaaa gagaacgaaa aaattatgaa tcaggcgctt tatgcacgcc cgaatgggtg
    60181 acccaaatta ttaatgataa agttaaagaa ttagcagcgc aaaaacagga tcttattttt
    60241 actggttcgc cacgtacaat ttatgaagca gaaaatgaaa ttccttattg ggaaacattt
    60301 tatggccaag aaaatattca tcctatttta attaatttaa aaccagaaac ttcagttttt
    60361 cgtaatacgc atcgccgagt ttgtgaaaaa tgcggatacc ctgttattta ttcgtgggag
    60421 actgaaatgt ggaaattctg tccattatgc ggagggaaaa tcattgatcg aggtgttctt
    60481 gatgatgagg caacaatcaa ggtgcgctta gaagaatatg aaaatcgtac caagcccatt
    60541 ttggcttatt ttcaaaaaag aggttatcaa gttattgaag ttgatggcga gccgttgcca
    60601 gatattgtta cccaaaacct tattgaagct ttggccaaaa ttaacttttt tgattaaaat
    60661 gattaaatta aaaagcgacg aagaaattca gaaattaaaa gaaggagggc gtctgctttc
    60721 aaaaattatt tatgaagtag cggcaaaagc aaaggcgggt atttcaacta gagaactgga
    60781 ttggttagcc gaaaattcta ttttaaaatg tggcggtgag ccagctttta aaaactatca
    60841 accagatttt agcttaaagc cttttcctgc tactctttgt gtatcagtta ataatgtaat
    60901 agttcacggc attcctggca attatttgtt gcgtaatggt gatttggtaa agcttgatct
    60961 aggaatgaaa tataaaggtt tttttacaga tatggccatt acagtgggag taggaaagat
    61021 ttcgtctttg gctcaaaaat taatccgcgt gactagaaag gctttagatt tggccattca
    61081 agctgctcaa gtcggagcaa ccttggggga tattggttat gcaattgaac aatatgttcg
    61141 ctccgaagga ttttatgaaa ttcgggtgtt gtctggtcat ggggtgggtt atgctccgca
    61201 tgaggatcct gatattttga attttggtgt tcctcataag ggtattaaat taaaggaggg
    61261 tatggttatt gcaattgaac cgatggtagc agttgattct tcggatgtta ttgaattagc
    61321 tgatggtagt tttgtgacta aaaccggttg tttggccgct cactttgaac ataccattgc
    61381 gattactagt cgaggaccca ttattttaac caaataaaat ggcgaatgtt ttagctattg
    61441 attatggaac aaaaaggatt ggattggcaa ttgcagatga aaaattgaaa attcctttac
    61501 cgcttaaacc tattatagaa aaaaaccagc agcaggtttt ggaaaaaata gcaaatcttt
    61561 gtaaagaaaa gaatgttgat caaattgtta ttggactgcc actttctttt gaatttgaag
    61621 aaacatccat gtgtcaagaa attaaagcgt ttggggagaa tctaaaacaa attactggaa
    61681 taaaaattat ctatgagaac gaggtcttaa caactgaagc ggcaaaaaaa atgaggcaaa
    61741 ctacttcgca gttaaaatcg cgaaaagaca aatcaaaaaa ccattttgat agtcaagcag
    61801 cgagtttaat tttggaaagt tatttgagaa aggttaagtc ttgattttat atttataata
    61861 tggtataata aaatatatga aacgaaattc attttacatc atagtcatca ttattcttgt
    61921 catcttattg gcatttgtca tttatgccta cctttctgat tatcagaagg cgaaagaaaa
    61981 tttagcagat cgaataattt ctgctcattt agaaggtttc cccgcgggga cgtcggtgaa
    62041 agaaggaatg cagggagaag aaaagactgt ttttcgaaaa ggggaatgga tgggtgtctc
    62101 gggaaaaatt aaaactaatg aaggaggaga tttgttttta aaaatctatg atttagctgg
    62161 taatttgatt caggaaaatg aaaagtggca taaatctctt caggctggcc agggcgattt
    62221 tggaatgtgc tgcattgtgg ttcccaatga agtcggtcaa tatgaagcgc gcttgatttt
    62281 aaacgaagaa gtcctttact ctttaaattt ttctgtaacg gagtaaaata tagttagagg
    62341 tttaaaaatt tttaaaagga gaatgaaatg aaaaaatatt tcatttcttt tgtggaagaa
    62401 ttcattttta atccaaagta tcaagatcat atagccggac gagtagaaat atatttgaga
    62461 aaaagtcagt atgatatcga tgagttgaga ttctttacta aaaagattaa gaaatttaat
    62521 cgttttcgta acaaatggga cttggaagaa gtttcggaat ctcaattaga aaaaattaga
    62581 aaaattattg caaaagaatt taatgaaatt gaagagtaaa aatatcaacc gcttcggaat
    62641 ggggcggttt ttttataata ttaagaataa atcgaataaa aaaccaaaga agcattttta
    62701 ggaagctcta ttttttattg ccgtttttag tcagcgttga cttttgtgtt tttaatttta
    62761 agtttataat atattcatat ggctaagcca tttctttctg ttattattcc tgcttacaac
    62821 gaagccaagc gtttacccaa aacgctttta gatatggata aatatttaac tcaggcaggt
    62881 ttttcatatg aaatcattgt agttaatgat ggttcaaagg atcatacggc agaaattgtt
    62941 accaaaatgc gggaatttat taaaaacctc tatttgattg ataacaaaga taatcaagga
    63001 aaaggagggg tggttgctca aggaatgttg gcagcgaaag gaacattacg attattttct
    63061 gatgctgata attccacatc ccttgatcag gtggaaaagt tctttcctta ttttaaacaa
    63121 ggatatgacg tagttattgg ctctcgttat gttaaggatt cagaattacc ggtaaaacaa
    63181 cctttttttc ggcagttgcc cggtagaata ggaaattttc tcattcgtct tttagctgtg
    63241 ccgggcatta aagatactca atgcggtttt aaagctttta ctgaagaagc agcgtcaaaa
    63301 attttccctc aattaacaat aaaacgatgg ggttttgata ttgaaatttt agttttagca
    63361 cgactaatgg gttttcgtat taaagaagtt ggagtgcgct ggattaacga tcctgattct
    63421 aaagtaaaag cgtcggccta tattgaagtt ttaacagaat taataaaaat tcgacttaat
    63481 atttggcgga aaaaatataa aataaattaa atgaaaaatt tgcggaagaa ttcaaaaaag
    63541 agaaaattaa ataatcatct cgtgtatatt tctgaactga gacgcgatcc agtttcgaat
    63601 gattgggtta caattgcttc ggttcgcgcc aaacggccag agcaactaat tattaaaaag
    63661 ccgcaaagaa ttgttacgcc taaaagccaa tgcccttttg aaaatccagc ggtgagcggg
    63721 cacaaaccgc cagaattaat aaaatctctt cctcataaaa aggattggtt tttgcagatt
    63781 tttgaaaata aatatccagc ttttcaacct aaaaatattt ctattagcga aagaaaatac
    63841 ggtccttata ccattatcaa tggttatggt tatcacgagc ttttggtttt aagagatcat
    63901 tttaagccaa tagccgatta ttcaacatcg gaattagcct ttattcttca agctcttcaa
    63961 gaacgttatc gacaaattgc ccaagacaaa aaaattaaat atatttctat ttttcacaat
    64021 tgggggtctt cggctggcgc ttcaatttat catcctcatt ttcaaattat cgctattcct
    64081 gttataccac cgcgcattaa tcattcacta atagacagta agcatttttg gaaaaaacat
    64141 cgccaatgcg tttattgttc aatgaacaaa tttgaaattg ctcaacaaaa gcgcattctt
    64201 tttcaaaata aaaaagcaat ggctttaagt ccttttgtct cggttgagcc atttgcttta
    64261 cgaatcattc ctcttaatca cattccttat tttgaggata ccccttcttc ggcgcttaat
    64321 gaagtggcgg aagttttgag aaagagtctc caagttttaa agaaaaaatt agatgatccc
    64381 gattacaatt tttttattca taccgcaccg actttcaaaa agaattctta tcaatactat
    64441 cattggcaca ttgaaattgt tccaaaaatt tctatttctg ccggttttga attatcaact
    64501 ggtattgaaa ttacagctat tgatcctgac gaaggcatta gatatctaaa aagtaaataa
    64561 ttatgattga ggtcatattt tcagcaattg ccaactttat tttaaattta atatctcaaa
    64621 tgggttatgc gggcgttttt attttgatgg cactcgaaag ttgtaatatt cctattcctt
    64681 ctgaaattat aatgccattt tcggggtttt tggtttctca aggtcgattt aatctttatt
    64741 ggttggcatt aatagggagt ttgggtaacc tgataggctc gttggtttct tacgggattg
    64801 cctttaaatt aggtaataaa gtcaaagaat tctttttaaa atcgaaattt ttcgctaccg
    64861 attatttaaa agccgaaaat ttttttaaaa aatatggcgt tagatctgcg ttgattgcac
    64921 gtgttttgcc agttattcga accttcattt catttccggc tggcattttt aaagtttcat
    64981 tgggcaaatt ttccgtttat acttttttcg gctctttaat ttggtcttat gtgttggctt
    65041 atattggatt gtattttggt gaacagtggc aaattatcga aaattatttt caccgatttg
    65101 attacataat tttaattgta attgttgggt ttgtatgcta ttatatttat agaaaggtaa
    65161 aacaaataaa cagtcgtaat agccataata attcaattga aaattgatat gactaaaaaa
    65221 tacaaagtag tagttactcg tcgaattccc gaagcaggtc ttcggttgtt aaaaaaggct
    65281 ggttttgaag taaaagtgag tccctatgat agaattttaa ctcgtcagga gttgaaaaaa
    65341 tttgttaggg gagctgatgc gattctttcc cttttaactg ataagattga cggcgaaata
    65401 ttagatgctg ctggttccca attaaagata gtggctaatt atgccgttgg ttacgataat
    65461 tttaatattg atgattttaa agcaagaaaa gtttatgcta ctaatactcc gggtgtttta
    65521 actgaaacag tagcagaaca taccatcgga ttgctttttg caattgctca aagaatcgtt
    65581 gaagctgatg aatttgttcg tcaaaataaa tttagagggt gggaaccaat gcttttttta
    65641 ggaacagaca taagaaataa aactttaggg attgttggtt taggaaggat tggaggagag
    65701 gtggccaaaa gaatgcgcga tggcttcgga ttaaaaatta tttattttga tgcgatgcga
    65761 aataaaagtt tagaaaaaca gttgggcatt caatacaccc cattcaagtc attattaaaa
    65821 caatccgatt ttattagcat tcatgtgcca ttgacgctaa aaacaaaaca catgtttact
    65881 ttaaaagaat ttagaatgat gaaaccaacc gcttatataa ttaatacatc tcgaggagca
    65941 gttattaaag aggaagattt ggcaaaagct ttgaaggcaa aaataattgc tggagcagca
    66001 ttagatgttt atgaatttga accaaaaatt acgccggatt tactaaaact acctaatact
    66061 gttcttttac cgcatattgc ttcggcaagt attgagacgc gttcaaaaat ggcagaaatg
    66121 gcagctaaaa acatcatttc tgttttaaga gggaaaaaac ctattaaccc cattttttaa
    66181 gttatccaca aaagcttata atttaaagat tttttggtta taatataaat aagaagattt
    66241 tttaaaggtc gattaattaa tatctaattc aaatatggtc gttcaatctt gggcagatat
    66301 tttagtggcc tctttccaaa cttggtggag tgcttttgta aattttctgc caaatttttt
    66361 aggtgcgctc atagtttttc ttattggctt gctcattgca gctggtttgg gaagtttagt
    66421 taataaaatt attactgcta ttaaattgga taaattattg tccaaattag gtgttgataa
    66481 gttttgtgag cgtgcaggtt ggaagcttaa ttccggtaaa tttttaggcg aagttgttcg
    66541 ctggttcttc attttagttt tccttttggc agcaaccgat attttgaagc tgacggcaat
    66601 tagtggattt ttaaaggacg ttgttcttta cttaccgaat attgttattg ctataattat
    66661 tatcattgct tcgattattg ttgctgactt tctcgcttct ttagtaagag gaactgtaaa
    66721 aggggctaat ttgtatgctt ctcagttttt gtctagctta actcaatggg tggtgctcat
    66781 ttttggattt ttgactgctt tgctgcaatt gggagtaggc gtcactatta ttaataccgt
    66841 aattacaggc gtgattgcaa tgtttgctat tgctggcgga atcgcttttg gtttaggagg
    66901 caaagattat gctgcttatc ttgttaatga atttcgtcaa cgaattgaag agaaaaaata
    66961 gatagcaatt ttttagccgc cccttcgggg gcggcttttt atttcataaa aacacaatta
    67021 ttttaatgcg taataatttc tgagaaattt tttaaacctc ttgacattaa aaaatctttt
    67081 aataaaatag aactataaac aatgaataaa tcattctaca ttatcttgat tcaagggagc
    67141 taaatacgag ggaaattttt tggcaatcat ccgcttcctt gaagaaagcg gattttttaa
    67201 aaataaaaag aaatatttac tatctggatg taaacaaaaa acttttgttt acatccaggt
    67261 tgtgaataac aaccgtgctc tttaacaatt aagaaattag gtacaatgaa gcatttttta
    67321 tcgggacgaa ataagggcgt atggtggatg cccagactag aaaaggcgat gaaggacgtg
    67381 gcgtagctgc gataagtctc ggggaggtgc gtagcaacct ttgatccggg aatttccgaa
    67441 tggggaaacc taatactgca aaccagtatt atatgccgtt tttgcggcat aagcataccc
    67501 gctgaagtga aacatctcag taagcggagg aaaagaaaac aaggcctcga ttttcgaggc
    67561 aaacatttcc ttagtagcgg cgagcgaaaa ggaaagagcc caaacctatt tatgttttca
    67621 taaatagggg ttgcaagaca gatacgcttt ttaatcatct tttagattca actcgagtat
    67681 taaaagatta ttaaaagtgg gggagtcaca aattttttgg ttagccgaat gaaatggaaa
    67741 gttcaaccaa agaaggtgat agtcctgtag gtgaaaacca aaaaactctc tagtatttgt
    67801 tcttaagtac ttcgagaaaa gaaaacctcg gaggaagctg ccagaactaa actggcaagg
    67861 ctaaatactt ttctagatcg atagcggacc agtaccgtga gggaacggtg aaaagtaggc
    67921 cggtgaggcc ggtgaaatag aacctgaaac catacgctta caaggaagcg aaaccctctt
    67981 atgtttttat taacataaag ggcgacgctg tgcctattga agaatgagcc aacgagtgta
    68041 tagatgctgc tttggttaat cctgctcagc gggagaagcc atagggaaac cgagatttaa
    68101 tagatcgtcc atcttttctt cgcaataaat ttattttatt gcgaagagat gatgaggtag
    68161 catttatacg acccgaagcc aggcgagctt actatggcca ggatgaactc cgacgaaagt
    68221 ctgagggagg tccgaaccgg ttggttgtgc aaaaccttcg gatgagctgt ggtaagaagt
    68281 gaaaagctaa tcgagcctgg taatagctgg ttctctccga aatagcttta gggctagcga
    68341 tcatccattc aatttttggg ggtagagcta ctggtttaat cgtaaagggc gaaagctcga
    68401 cgattaagtc aaactccgaa taccaaaaac gaggatgatc agtaagactg cgggggctaa
    68461 gcttcgtagt caaaagggta agaacccaga tcgccgtcta aagcctctaa atcttggtta
    68521 agtgttaaag gaagtgatta gccgaagaca gataggatgt tggcttagag gtagccacca
    68581 tttaaagagt gtgtaacaac tcactatcat attccgactt cggtcggaat tggctgatcg
    68641 cgcctaaaat gtagcgaggc ttaaaccaag tgctgaagac gcgattcctt atgttttgca
    68701 taaggaaggt aggagagcat cctaacatct gtgaagtagg attcgagaga acctatggag
    68761 aggttaggag agagaatgtt ggcatgagta accgcattcc caatgagaaa ttgggacgcc
    68821 gaaaatccaa ggtttccgtg gcaatggcaa tcatccacgg gttaggcgat cctaaggcaa
    68881 tggcgaaagc cgcagctgat ggagagctgg ttaatattcc agcccttcct ataaattcga
    68941 tggagggacg aaggaaaaaa atctgagcat cttattggtt tgatgttctt gatgagtaga
    69001 aatgtttgtt aggtaaatcc gacaaactgc tttaaatagc aaatttcaat catcgagaaa
    69061 atttcgcttc ggcgaaaaat tcagatgcag agccttccga gaaaaacttc taaggttagt
    69121 ttataggaat tcgtaccgga aaccgaaaca ggtggattgg tgtaagtaca ctaaggcgaa
    69181 cgggtgagtt ctcgttaagg aactcggcaa aaaagcggcc gtaagttcgc gataaggcct
    69241 gcctacgtta agtaggccgc agctaaagaa cctctggcga ctgtttaata aaaacacagg
    69301 tccctgctaa ctcgtaagag gatgtatagg ggctgacgcc tgaccgatgc gagaaggtca
    69361 agagtgacag ttgatttgtc gcaagacaaa tcagccgatc gctttaagcc ctcgtcaatg
    69421 tcggcggtaa ctataaccgt ccaaaggtag cgcaattcct tgtcgggtaa gttccgacgc
    69481 gcatgaatgg cgtaacgatc agaggactgt ctcaacgaga agcccggtga aaatgcgatt
    69541 ccagtgaaga cgctgggtac ctgcaacaag acgaaaagac cccgggagct ttactgtagc
    69601 ttgatattgg gtttaagttg ttgctgcgta gcataggtgg gagactgtga agctccgatt
    69661 ccggtcggag tggagtcgcc agtgaaatac cactctgtaa caatttaaat tctaacttta
    69721 gcggtaaaaa cgctagagga cagtgtctgg cgggcagttt ttctggggcg gaatcctccc
    69781 aaaaggtaac ggaggagttt attaaggtcg gctaggtgcg gatggaaatc gcactggtcg
    69841 tgtaaaggca taagccggct taactgtgag gcagacacgc caaactcgag tttttattaa
    69901 ctcgacggtc cgtaattttt atttacggat tgagatgcga aagcagaact tagtgacccg
    69961 acggttcttt ttagaacggc cgg
//
LOCUS       DAOSMC010000002        44197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_1104_length_44197_cov_20.765439, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000002 DAOSMC010000000
VERSION     DAOSMC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 44197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44197
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1104_length_44197_cov_20.765439"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1265)
                     /gene="ychF"
                     /locus_tag="PL075_00405"
     CDS             complement(198..1265)
                     /gene="ychF"
                     /locus_tag="PL075_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOD96703.1"
                     /translation="MKLSIGIVGLPNVGKSTLFETLTQKQVDKANYPFCTIDPNIGVV
                     AVPDERVDLLADLTHSQKKIYATIDFIDIAGLVKGASQGEGLGNKFLANIRETDAILY
                     VLRAFANNKIINTQNSIDPLRDKDILDTELILKDLETIEKRLNNLEGDIKRGNKEAMR
                     DYAALSKAQKILEEGKILFEQSFTEEELKELNKYQLLTSKPRLYLLNGKDEEVPQNII
                     NEFNKNRWPYLIIDLLTEYEAVNLSKEERKELGLPTDTELDLLIKKSYEILDLITFFT
                     TGPDETRAWTIKKGTKAPQAGGVIHSDFEKAFIKAEVINWKDLLDAGGFAQAREKGLI
                     RTEGKNYVVQDGDVIEIKHNA"
     gene            complement(1295..2134)
                     /locus_tag="PL075_00410"
     CDS             complement(1295..2134)
                     /locus_tag="PL075_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD96704.1"
                     /translation="MNTNQQKVVKYFTNPETKLGFNYVLWGSKHFGFYPTGKADITEK
                     EAQILYQDLVAENLQLKEEQIVLDAGCGQGIVSTYLAKKIGSNVFGITLVPFEVQKAQ
                     KRAKKLGVQDKTDYQIMDYSSTNFPDNYFDAIYTTETLSHSPDIRKTLNEFLRILKPG
                     GKFALFEYTIAPDKKFSPWEKKMLDVVIEGSAMMGLKDFRHDQFTEVIKSVGFKNVKE
                     RDITENVLPSFYRLHKLSLWAYWFVKLFGLQKFLINTTAGYEYYKMATKGLLRYCIFT
                     GNK"
     gene            complement(2245..2331)
                     /locus_tag="PL075_00415"
     tRNA            complement(2245..2331)
                     /locus_tag="PL075_00415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2295..2297),aa:Ser,seq:tga)
     gene            2510..3064
                     /locus_tag="PL075_00420"
     CDS             2510..3064
                     /locus_tag="PL075_00420"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD96705.1"
                     /translation="MTASVLYITFLIIEIILMIAELFLIILFISLFFYRIKTGVPFVK
                     SKKVLVEKIKEANILKDDTTFCDLGSGDGSFLYELAKEFPHIHFIGYEINIFLYIFSK
                     LFNHLPNIKFYHQDFFKVDLNQFDYIYAFLLSPLMERLVPKIQKEAKKSCYVIVNTFA
                     FKNLKPVKEIGSANPLESLYFYQL"
     gene            3061..4332
                     /locus_tag="PL075_00425"
     CDS             3061..4332
                     /locus_tag="PL075_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOD96706.1"
                     /translation="MKIVADFHIHSRFSRATAHDITVVNLNQWMKKKGVNLLGTGDFT
                     HPQWFKELKENLEPAEPGLYQLKNDLNEQNFSRFILTSEIACIYSQGGKTRRIHHVIL
                     APSFEVVQKINVALSWQGNLTSDGRPILGLSSIELAKLLFSISSEIVLIPAHIWTPWF
                     SLFGSMSGFDSLEECFGEWSSKIFAIETGLSSDPQMNWRLSQLDHLAIISNSDSHSTS
                     KIMREANVFDTDLSYEKIIEAIKSGTPQNYAHDPSKFLYTIEFYPEEGKYHYDGHRLC
                     NIVFSPEESKKHNNICPKCNRPLTIGVMHRVEDLADRDQNYVASDRPPFKKLVPLEEI
                     IAEVLKTSTTSKKVKAEYERLIKVFGNELTILIDATIKDIEKESSPELAEGIRRVREN
                     RLSIAPGFDGEYGKIKIFVDDEDIPSNSTLF"
     gene            4335..5438
                     /gene="tgt"
                     /locus_tag="PL075_00430"
     CDS             4335..5438
                     /gene="tgt"
                     /locus_tag="PL075_00430"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOD96707.1"
                     /translation="MFRLIKKSKKSEARLGQIKLASGTIKTPCFMPIATRGSVKSVTS
                     ADLEKIGAEIILANTYHLFQRPGDEVIKKFGGLHQFMNWRKPILTDSGGYQVFSLAQL
                     RKISLAGVEFNSEVDGQRIFLSPEKALEIQKNLNSDIAMILDYCVGYPAKKAEVARAM
                     QLTTLWAERSRKYADKIKFKNQIFGIIQGGIFKDLRSQSLQNLMNLNFDGYAIGGLAV
                     GEPEKKMKEIIKWLAPQMPANKPRYLMGVGYPEQIFEAIQNGVDMFDCVIPTRHARHG
                     ELFVRTNKQIVISRRLNYQIISIGKSKYQSQNLPVDRFCHCETCANYSLAYLHHLYKN
                     NELLYYRLATIHNLKFYLDFIREVRLAIKNNLI"
     gene            5435..6025
                     /locus_tag="PL075_00435"
     CDS             5435..6025
                     /locus_tag="PL075_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOD96708.1"
                     /translation="MITKPKLVLHVCCAVCGAALVKVLQEKFQVILFYYNPNIWPREE
                     YEKRLEAVVKLSEIYGVILKKGKYENKLWLKKVKGFENEPEGGKRCPICFQMRLEKTA
                     DLAKSSGALYFATTLAISPFKNEAVINEIAQEVAKDKGLIFLSLADIGDKNNLWQETR
                     LLAKQYQLYHQKYCGCQFSLAAMSKKQTLAKNKKHL"
     gene            6033..6383
                     /locus_tag="PL075_00440"
     CDS             6033..6383
                     /locus_tag="PL075_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96709.1"
                     /translation="MTLDRFFIFYLIALAFNRIKLFLKHWYSNSFIKFWNFFIQTLRN
                     LDRTFAIKITARHWLDPLYQDRTVVGYILGFIFRTLRIAIAAVLYLAIFIVVMFGYLI
                     WAATPILLIVRAFA"
     gene            6396..8918
                     /locus_tag="PL075_00445"
     CDS             6396..8918
                     /locus_tag="PL075_00445"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOD96710.1"
                     /translation="MTVKGKVEQFFYRDPQFEMSFFERILVRFFIFLFWSLAVVLTIM
                     LILMRESSLRFLGYFGIIFILDRIVNFDRPDKNFDKSFDQKLVTQPNCKINLLPYSEA
                     PVKRVLVNAFEKTLSQGGNFNFQLLLEFLEERKFREIFERLEINCNELESAIRENLKK
                     NFTRPSKKSLIKTINDLVVSAYFLRDKNYLQTRDLFVALFYIKDEELERIKNYFRIQS
                     DDIRIAAVYSEFKKQFGRFLTPRRRRELVKQTKQEKHKIMNRAWTARPTPFLDSVSDD
                     LTDLARAEKIGFLIGHDGEVRQLINILSRANRNNALLVGEPSSGIGAIVRHVAYLIVK
                     DEVPSELFDKRLVALSIDELVSGCEPQAVAERVQRIVKEILDAGNVILFIPDIHNLFK
                     TSGQNYLSAAEVLLPILSRSSFQVIGATTPGIFKTEIEPREDFMSLFEVILVNELSEE
                     DAILFLTYSSLILEKTYKIKITYPAIKNAVKIAHRYFRDKMLPTSADDLLKEVLADVR
                     NLKQKVLTGEDVIRVSQEKINVPLKFAEKEEAEKLLNLEKIIHERLIDQNEAVKEVSK
                     ALREYRAGLSRKGGPIATFLFVGPTGVGKTELAKILAKIQFGSENNMIRFDMSRYQDK
                     KSIFDFVGAPEGDRPGALTEAVRQNPFSLILLDEFEKAHPDLMNIFLSVFDDGRLTDN
                     LGRVIDFTNTIIIATSNAHSALIKERIETGVAMEQIAAELKQKLTDYFQPELINRFSD
                     IIVFKPLSPDDLIQIARLNLADLAQSLATEQGIKLFFDDKAVKQLVKIGYDPVYGARP
                     LRNAISDNVKAVLAEKILRGEIKRGDELMLTFEGEQFLLLPR"
     gene            9132..12431
                     /gene="rpoB"
                     /locus_tag="PL075_00450"
     CDS             9132..12431
                     /gene="rpoB"
                     /locus_tag="PL075_00450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOD96711.1"
                     /translation="MPNQKIFSHFPRSLQTLEYLPHLADIQIKSYEWFLKEGLRELFD
                     EISPVKDYTGKDLELYFEDYYFDEPKFNEIESQEKNLTYEAALRLKLKLINNKTNEKK
                     NQEVYFGEIPMMTSRGTFIINGVERTVISQLIRSSGLYLTAIQSRGRKLFGAKIIPNR
                     GAWLEFETDLDSVIYVKIDRKRKVPVTCLLRLFGLENKESILKTFANVDTGEIKYIEK
                     TLAKDVATTRSEALVEIYKRIRPGDLATPENARGLIEGMFFREDRYDLGEVGRFKLNQ
                     RLNRASKEADTKSRLLSLDDLIDVVKLIISSNNNEAAEPDDIDHLSNRRVRAVGELLQ
                     NRLRVGIMRMRRTIQDRMSTLDIYSLVPAQLINARQMMAIIWEFFSTSQLSQFLAQEN
                     PLAELEHKRRLSAMGPGGLHRKRAGFEVRDVHPTHYGRICPIQTPEGANVGLVTYLSS
                     FARLNRLGFIETPYFRVKKGQVTDEVVYLDAYEEEKYYIAHSNINIDNKGKIIDDIVE
                     ARYKGEPGFYPREDINFIDISPFQLLSVATSLIPFLEHDDANRAQMGSNMQRQAVSYM
                     SSEAPLVGTGLEMKAAHDSGHVLVSDVEGEVVEVDGAHIKIKIKGTSNFKNYRLRNFI
                     RANQYTCISQRPIVTKGQKIKKGDVLADSLQTKNGKLALGKNLLVAFVSWGGANYEDA
                     VILSERVVRDDLFTSIHIEDFVCDVRETKLGPEMTTLDIPNVSLEKLKDLDEEGIVRI
                     GAEVKSGDILVGKISPKGEGDLTSEERLLRAIFGEKARDIKDTSLILKHGKRGRVVGI
                     RIFSRERGDKLEPGIIKRIQIDVAQRRLIMAGDKLAGRHGNKGVISKIVPEENMPYLA
                     DGTPVDIILNPLGVASRMNLGQILETHLGWAAHKLGYQAIVPVFSGTTEEDIKNEFKA
                     AGLPENGKATLYDGRTGEPFSEPIMVGYIYMMKLVHMVEDKIHMRSIGPYSLITQQPL
                     GGKAQLGGQRFGEMEVWALEGYGAAYALQEMLTIKSDDIRGRIAAYEAIVRGQDFKSP
                     NIPASFHVLVSELKSLALNVELIGVKGKVHALDESEEGKLFAEAMVDNKYLTENNQNS
                     KQGQDNQNPAN"
     gene            12433..16098
                     /gene="rpoC"
                     /locus_tag="PL075_00455"
     CDS             12433..16098
                     /gene="rpoC"
                     /locus_tag="PL075_00455"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOD96712.1"
                     /translation="MLPEDFYAIKLKLASPKEILSWSHGEVTKPETINYRTQRAEKDG
                     LFSERIFGPTKDWECYCGKYKRIRYKGVVCDKCGVEVTRSSVRRERMGHIALACPVAH
                     TWFLRSIPSQIALFLGVSQQQLERVIYYTAYIITKINNEEKKKIQAELQEEYNAKRKK
                     TDKINMLDTLYHHHINELNSLEPLKIIPETEYLELSKRYGHIFEAGIGSEPIVKLLEQ
                     IDLKKKERELVKAFKTQKGDKKKILLQLKLVKSFLKNNLRPEWMFLTIIPVLPPDLRP
                     MVALDGGRYATSDLNDLYRRVINRNNRLKKLIELNAPEVIIRNEKRMLQEAVDALIDN
                     GARKTQIQSSAQKRPLKSLSDILQGKQGRFRQNLLGKRVDYSGRSVIAVGPELKHYEC
                     GIPKTMALELFRPFVVNKLLERGLVFNIRNANRLIDQGPAEVWAILEEVIKDRYVLLN
                     RAPTLHRLGIQAFKPVLIEDLAIRIPPMVCTAFNADFDGDQMAVHLPLTQEAQKEAKE
                     MMISSRNFLKPANGEPIVNPTKDIILGCYYLTKIWDNRKGEGKIFSSPEEAMLAYENK
                     IIDLKARIKIRPYKEHYLKQMGGVLVVEKYENPNTLIDISVGRIIFNEIFPKDFPLVT
                     AVVEKKFISQLTDFIIKYFESDKVIEVMDKIKEIGFNYATRSGITWSMSDLVIPAEKK
                     EIIDEARKEINQIEQQFNQALLSKEEKRVHAIKAWENSISKISKNISKVLPPDNAVFQ
                     IFDSGSRGSWGQLSQMMGMKGLVQNPKGEIIELPILSSYKEGLTVLEYFIASHGARKG
                     SSDTALKTANAGYLTRRLIDAAQDLIIIEEDCGTTSGITVYRDDGAEYGHHFSSKIVG
                     RTSLEDIKIKNKLIVKANEIINAEAAELIEESTIQQIKIRSPITCKTLWGVCSKCYGN
                     DLAYNTPVTLGTPIGIVAAQSIGEPGTQLTLRTFHTGGVAGQDITHGLPRIEELFECR
                     IPRNKAVISEVNGTVVKIEERDKDIAIQIKPDLIRKEHSKKDKKDAKTKEIGLEEYLI
                     PRTKRILVNEGDKVVVGQKLSFGDIDLKELLRVTDRYTVERYILNEIQRIYVSEGVSI
                     NNKHIEIMIRQMFDKVKIKNPGDSDFLVGDVVSKSRFLEENKKLKSKGKKPARAIQLF
                     LGITRSALFSESFLAAASFQETRRVLVKAAIEGRVDYLRGLKENVIIGRLVPVGTGYR
                     KRLEEKEKEEKSKKKEN"
     gene            complement(16140..18371)
                     /locus_tag="PL075_00460"
     CDS             complement(16140..18371)
                     /locus_tag="PL075_00460"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOD96713.1"
                     /translation="MLDEKVFSLDVGGRILTVKFTPLAQQANGSVLVQYGETTVLITA
                     TMGKTDRLENDFFPLVVDYEERFYAAGKILGSRFVRREGRPSEGAILTARLIDRSIRP
                     LFNQNMRREVQVVATVLSIDADNDPDFPALIGASLALATSDIPWSGPISAIRIGLVNN
                     EFKVNPTYQDRNSGQLDFFLSSADNKITMLEAGGDEVPEEKIVQATQLALIEIEKLQK
                     FQSEIISQLAKPKTDIVLKQINPDIRTKTKEFIQDKLEDAIYTPVKIGRVSKLNELKK
                     NLEEKLKSENFDANAISEAFVVLEEEIDALVHKNILEKEKRPDSRALDEIRPLYCEIG
                     LIPRVHGSGFFMRGSTQALSIVTLAAPSHALTIETIETCDTKRFIHHYNFPPYSVGEI
                     SSMRGPGRREIGHGALAEKSLYPIIPNKNEFPYAIRIVSEILSSNGSSSMASVCGSSL
                     ALMDAGVPIRRPVAGIAMGLMLDNESHYKILTDIQGPEDHYGDMDFKVAGTTEGITGI
                     QMDVKITGITLQILEETLIRAKKARLQILQKIAEIIEKPRPSLSPHAPKIFTLQVNPD
                     KIGNIIGSGGKTINDIIKRTGVEIDIDEDGQVFITAPTDESGQQAIEIIKGLTYEFKE
                     GEVVTGTVSRILDFGAIIDLGYNRDGLLHISEIANRRIDKVEDVLKVGQILTLKIIKI
                     DETTGRINLSLKALQPSTSADSRANQVLHEKSSEQHSTRRGQSSYRHDRRNQPGSRPR
                     RRN"
     gene            complement(18486..19028)
                     /locus_tag="PL075_00465"
     CDS             complement(18486..19028)
                     /locus_tag="PL075_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOD96714.1"
                     /translation="MIIKYKDQRVAIFFDIQNLYHSAKNLYHARVDFYEVLKAATANR
                     KLVRAIAYVVKSDAVEGESSFFEALERMGIELRMKELQVYPDGLKKADWDVGLAVDAI
                     RLAPNIDGIILVTGDGDFIPLVEYLQALGKPVEVVAFGRSASAKLKEIADEFVDLEQN
                     PQKFLIKSPLIKKSKSRFQR"
     gene            complement(19040..19303)
                     /gene="rpsO"
                     /locus_tag="PL075_00470"
     CDS             complement(19040..19303)
                     /gene="rpsO"
                     /locus_tag="PL075_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOD96715.1"
                     /translation="MLKKEEKSKIVSKHRQHEKDTGSTEVQVALLTKQIDKLSEHLKK
                     HSKDNSSRRGLLKMVSKRKKLLNALKNESPTRYEKLTRSLNLK"
     gene            19379..20323
                     /locus_tag="PL075_00475"
     CDS             19379..20323
                     /locus_tag="PL075_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOD96716.1"
                     /translation="MNQKSLLNLKNQYLEYLEIEKNRSAKTLENYNRYLTVFLNFLSE
                     RLHKKLEQLIPKDITLENVRQFRLYLNRTEGTEGNLKKVTQNYYIIALRGFLKYLSKM
                     NVESLPAEQVELARVTRNEIGLISFEDLERLLNAPDENSLKGLRDRAILEMLFATGLR
                     VSELCQLNRESVNLERGEFSVKGKGGKIRLVFLSDSAKAILKKYLEKRTDTEEPLFIT
                     FKKSKNNEVLGRITPRSVERLINFYARKSGIVKRVTPHTLRHLFATDLLQNGADLRSV
                     QMLLGHSNISTTQVYTHITDKELKEIYQTFHARRRKKP"
     gene            20361..21440
                     /locus_tag="PL075_00480"
     CDS             20361..21440
                     /locus_tag="PL075_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOD96717.1"
                     /translation="MDTKDLDTILKEFIGILSEKQREVIEKRYGLHGEKPMTLEAIGQ
                     EKGLTRERIRQIEEAALETLKKNLNELSDLIERIKYQLELLGGIRREEQLLEEASALL
                     TPKPVELWTSKAIRERWQNDINFLLDISPDFFYFFEKPDIYSNWYLREEFLKKAINIY
                     KFVEHELKITKRPLPLDQYQLLLRKTMKEFRIHNENILISYLDVSKKFSFNPFGEFGL
                     IDWELINPKNVKAKAKLVLRNANRPMSYQEITKAINELNFDGKRASINTLHNELIAND
                     EFVLVGRGLYALKEWGYRPGTTKDIIISILAKKPMTYREVLEEVKKQRLVKDTTVLIN
                     LQDKTTFKKLPDRRYCLIQSANKKN"
     gene            21440..22705
                     /locus_tag="PL075_00485"
     CDS             21440..22705
                     /locus_tag="PL075_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96718.1"
                     /translation="MAEALAILQAVLIAIGHFLGWVWWIVLPIIFFKADYRLWLDYVK
                     KKFLSKVEYVIFEIKIPKYIEKSFKSMEDVFSALHSIQISKPNFKERVLEGKTQLWIS
                     FEIIGNGGGAGFYIRTPKDYRHLVESQMYAQYPDLELTEIDDYTQVFSSMLPNKFWDL
                     WGLDFTFVKEAPYPILTYPFFEAQKEEKTIDPLSSLMETISKLELDEKIWIQLLIKPA
                     DDSCKKEGEKIVNKLIGKKEGKPRSYWDYILEFIYNLIKAPIQPPEWSEDKGTEGSPG
                     LMQHLTPGEREVVQAIQNKISKLLFDATIRFVYIDRQNAFTRLNITSTMGFFRQFNTL
                     NLNGFKPHKPSVTKGTWPFKKQKEFWKKTVMYKNYLKRKFNSDCKVILNTEELATIYH
                     FPVTVVEAPVVRRIEAKKGEPPINLPIAQ"
     gene            22851..24278
                     /gene="nusA"
                     /locus_tag="PL075_00490"
     CDS             22851..24278
                     /gene="nusA"
                     /locus_tag="PL075_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOD96719.1"
                     /translation="MFDLKEFFSAINEIAEEKGISQDKILEAIEQAVAAAYKKEYANK
                     NNIIRAKIDPETGEVKFWQIKIVVDESMLKEEEEGEAVEKEETSQLTATEKQIQQGEE
                     LAKKIRFNPERHIMLEEAVKIKPEAKPGDEIIFPLETHEEFGRIAAQTAKQVILQKLR
                     EAEKEAVLEEFKNKEGEIVSGIIQRIENRNIYINLGRVDGVMFYNESIPNERYRIGER
                     MKFYLLAVQDAGRGPMIVLSRSHPKFVSKLFELEVPEIASGVVKIMAIAREPGFRSKV
                     AVASTVEGVDPIGSCVGQKGTRIMAVLNELGQEKIDIIKWSDNPEEFVAQALSPAKVS
                     RVEMMPRREMRVFVPEDQLSLAIGKGGQNVRLAAKLTGWKIDVRSESAPEAKVEGGVA
                     EALEENFEEENSSQVAEQVSSETNKTINEQSLIKKQLSEKIQELLKSNGYDEERLKSA
                     TKEELMQVKGIGEKTAEKIIQIFQQ"
     gene            24297..25586
                     /gene="tig"
                     /locus_tag="PL075_00495"
     CDS             24297..25586
                     /gene="tig"
                     /locus_tag="PL075_00495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOD96720.1"
                     /translation="MKHTLKYLSQSQIELEVSLTAEEMEQFWQAARKKVLSEVRLDGF
                     RQGNIPEELIKNTDLEEKIFDEAANQAFKKSLKEVIDELDLELIGAPEVNIKKIAPKE
                     EFVYVVRSAILPKPELKNYKEISQKIFKEKKNLKVEKGEIQEAIEWLQKSRAKIVPVD
                     RPAQPKDVVEIEIKCLENEKEIADFPKLDRFILGEGRWPGDFEKQLEDMKVGEEKEFN
                     VIVPNDYHNENLRNKNLFFHVKLNGVFERQEPELTDEWAKTLGQFNDLNDLKKSIEEG
                     ILEEKETKEKERLRVALLEKLIEQTKIELPEILIEGETENQLHSLKHLIERQGMSFEE
                     YLNKIKKTEDDLKKDLRKEAEKTLKGFVVLRTIAKLENCIPSPEEIEKTVNEIINYQA
                     MEGNDVNQIDREALMEYTKEKLTNEKVFQFLENQDQK"
     gene            25588..26226
                     /gene="clpP"
                     /locus_tag="PL075_00500"
     CDS             25588..26226
                     /gene="clpP"
                     /locus_tag="PL075_00500"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOD96721.1"
                     /translation="MDFKKPQSKIKNLEEGYPLLIPTVIEKSQFGERAYDIYSRLLKE
                     RIVFLGGPINDAVANSIIAQLLFLEHEDSKKDIELYINSPGGVVTSALAIYDTMQYVK
                     SDVSTICIGQAASAAAVLLAAGAKGKRFALPNAEILLHQVMGGAEGQAVEIEITAKQI
                     LKIKNKINEILSKHTGQPLDKIEKDTDRDFYLSASDAKEYGLIDGIITKKKK"
     gene            complement(26358..26440)
                     /locus_tag="PL075_00505"
     tRNA            complement(26358..26440)
                     /locus_tag="PL075_00505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26404..26406),aa:Leu,seq:gag)
     gene            complement(26561..26935)
                     /gene="rplS"
                     /locus_tag="PL075_00510"
                     /pseudo
     CDS             complement(26561..26935)
                     /gene="rplS"
                     /locus_tag="PL075_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(26990..28087)
                     /gene="ychF"
                     /locus_tag="PL075_00515"
     CDS             complement(26990..28087)
                     /gene="ychF"
                     /locus_tag="PL075_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOD96722.1"
                     /translation="MLSIGIVGLPNIGKSTLFQAITQKQVAIANFPFTTIDPNIGLAP
                     VIDERLQKIAEIQKSKNVIPATVKFVDIAGLVKGAAQGMGLGNQFLSYIRETTAIAEV
                     VRLFSAPNIPHIEEKIDPLRDLEIISTELILADLEALGKAFKKIEGDLKRKEKTAQQK
                     FELIQKLERHLQNGQWISTFPLTDKEKELIKDFNFLTQKPLLVVLNVDDSITPVEIEK
                     IKEEIIDHIGNQVNKTDIFTINLAKELDLATLSNIEAAELNINRLPFLNKLDDLIKRG
                     YEILDLITFFTTNGPEETRAWPLKRGSTVWDAAGLIHKDFQDKFIKAEIVNWRDFIEC
                     GSWAKAREMGKLKVEGKDYIVNDGDIVYYKI"
     gene            28131..29450
                     /locus_tag="PL075_00520"
     CDS             28131..29450
                     /locus_tag="PL075_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96723.1"
                     /translation="METINKKIFLAYDIRGRHPKEINGDVLWRLGYNLPMILQKEKIL
                     PNRKLKIIVGRDSRPSGAILIKNLIQGLLQNDIELVDAGVITSPMLYFLIKMHHFDLG
                     FIITGSHLSESYTGLKIYTHELNTLSGNWLVQFLDIFNRPVTVGLTKGKLTRKNFINE
                     YVQFLLQKSKIRQFKKTLSLKNILICCPKSSQLILEKIKKELKLNLQFVHRPIPKRLI
                     AQRKCDCVIQFDSDADRMQVFNSKGEQILGDVIGAFFVELMKNKMPKKIILDCRSGQF
                     LINLARRYRFKIIYSPAGHGFFKQAMKKNKALLGIEKSGHYYWKDFFFADSGIFSFLM
                     LLQFLAGKKDKIDDVARQFFQGVISPEINFKIKNQRQVNKIFQKTEKAFSKNRINKID
                     GLTVFDKNFKFNLRVSHTEPNILRLNIEAINEKVFQEILSKIKQLVK"
     gene            29456..29827
                     /gene="rbfA"
                     /locus_tag="PL075_00525"
     CDS             29456..29827
                     /gene="rbfA"
                     /locus_tag="PL075_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOD96724.1"
                     /translation="MAASYRLKQLNSLIQQLFSEILQKESNFSEEVFVSVSKVEVSKD
                     LANAKVFISVFPAVTDEIKEYFKKNIFALQQLLNKKLDIHPVPKILIVYDDSEAKAAH
                     IDALLNQIKKDARRSRKRKKD"
     gene            29947..30018
                     /locus_tag="PL075_00530"
     tRNA            29947..30018
                     /locus_tag="PL075_00530"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29979..29981,aa:Cys,seq:gca)
     gene            30122..30207
                     /locus_tag="PL075_00535"
     tRNA            30122..30207
                     /locus_tag="PL075_00535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30156..30158,aa:Leu,seq:caa)
     gene            30813..31802
                     /locus_tag="PL075_00540"
     CDS             30813..31802
                     /locus_tag="PL075_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOD96725.1"
                     /translation="MKKEKMKKVVVIGGGTGVFVVLTALKNFPVHLSAIISMADSGGS
                     SGILREEFGILPPGDVRRALIALSNSDNKLLARLFNYRFNESYFLDGHSFGNLFITAL
                     ERITGDFQKAIREAGKILNISGEVIPVTLDNINLVAQLKNGQIIYGESAIDRRKEKNS
                     SKIQKIWIQPKAKINPLARQTILAADYIVIGPGDLYTSIIPNLLVSGVTTALKKSRAK
                     KIFITNIMTKKGETDDFKASDFIITLEKYLGKKILDYVLINNSRPDKKRFKKYKEEGA
                     QFVEPNVDQWGRALVIKKDLLRERGLLRHDPKKLGTTLGEIILAEKTYGKLIK"
     gene            31783..33300
                     /locus_tag="PL075_00545"
     CDS             31783..33300
                     /locus_tag="PL075_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOD96726.1"
                     /translation="MESSLNKNWLQKIISLPPPHQFLLITIIFIGGIFFSSFLNFQIA
                     IFIGLFIFLISAILGLSAILMQKRAIFFIYPLVFLLGFFYLQFYNFYQENHSSNYLNG
                     TEQSQVSAIISDLPIYNNNVSFQVQLLAPFQGKALIKTRSYTTDFHYGDKILISGKFE
                     QPQAYEDFDYRAYLAKDNVYSVIYYPEIKILAHHQGSWLKENLYRLKLSFQKQIDKIF
                     SEPESGFLAGLLLGEKRTMDKDLQLALQRSGTTHLIALSGYNITIIIQAVSFLLIALG
                     LARPQTFWAVVIFLIMFIMLTGASPSVVRAGIMGMILLLSNKLGKRYEARNALFCAAL
                     LMIILNPKIIRFDFGFQLSFAATLGLIYLMPFFGRLFKVDQASFLDWRKILATTLSAQ
                     IMVLPVLVLRFGSLPLISPLANVAIIIFIPATMLVGFLAVFLSFINLWLGHLIGFLGY
                     LLLHFEIAMINFFGHWNFAALNFGAFREIVFWASVIIIFAFGIYLKKHELIKQEI"
     gene            33275..34120
                     /locus_tag="PL075_00550"
     CDS             33275..34120
                     /locus_tag="PL075_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOD96727.1"
                     /translation="MNSLNKKSSKILIIALLIIFCFDTLLWIEILKEPKNVEADFLDV
                     GQGDATLLKFPFSGQILIDAGDGQKIKTALSQVQGYFNKDIDVWILSHANLDHYGGFL
                     KLLETNPPRIFIYNGFDSEGDSFQALKKLLHAKEIPILVLAAGDKIKIGNSRFDIIWP
                     TANFLRKDLNDTSLVLRFVDHNHSILFTGDVSAKILSSLSNIQSEILKVSHHGSKTGI
                     TASVLDMIKPRIALIGVGFNNRFGHPSVEVTSLLKKIGSQIFRTDLNGTIKIHFDENL
                     FIETQ"
     gene            complement(34121..35023)
                     /locus_tag="PL075_00555"
     CDS             complement(34121..35023)
                     /locus_tag="PL075_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOD96728.1"
                     /translation="MIKELHSKISWLDIVNPTEEELSDLQKRFHLHPLIISELKNPST
                     RTKIEVYDGLMFIVYYLSNYNEKTQISEAIEIDFLLTKDALVSVRYQNFKLIDNLFEK
                     IKNDPSNLLNKTPAHLLHYILEEGLTFSLRQLAHINGKIKKIEDAIFKSNPKSLIEQI
                     AIVKRDILDQRLIARPQHSILESLKERGVQFFGKDLEIYFNDLLGDYEKLWMNLDNLK
                     ETIESLENTNNTFFESRTNEIMKILTILAFITFPLTLISSIFSMNTAYMPFVNSPYGF
                     WYVIGLMVLLALLFLAVFKIKKWL"
     gene            35336..35746
                     /gene="rpsL"
                     /locus_tag="PL075_00560"
     CDS             35336..35746
                     /gene="rpsL"
                     /locus_tag="PL075_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOD96729.1"
                     /translation="MPTINQLVKKGRKKVVYKSKAPALLWGFNYKKNRPVKYPSPFKR
                     GVCLKVFTTTPKKPNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGVLDAVGVENRRQQRSKYGAKKPQ"
     gene            35752..36219
                     /gene="rpsG"
                     /locus_tag="PL075_00565"
     CDS             35752..36219
                     /gene="rpsG"
                     /locus_tag="PL075_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOD96730.1"
                     /translation="MRKKHRQKRTIEEDLIYNRVECTRFINYLMQRGQKATAEKNFYK
                     AMEIIKEKTKKDPMEVFDAALKNVAPIVEVKSRRIGGANYQIPQEVKPDRQLTLACRW
                     IIQAVRSRRGKPFYEKLAEEIIAASNNEGAAIKKKQDTHRIAEANRAFARFSW"
     gene            36350..38455
                     /gene="fusA"
                     /locus_tag="PL075_00570"
     CDS             36350..38455
                     /gene="fusA"
                     /locus_tag="PL075_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOD96731.1"
                     /translation="MREYSIEKTRDIGIIAHIDAGKTTVSERILYYTGISHKIGEVHE
                     GEAIMDWMEQERERGITITSAATTCFWTQTYKTPEERAKKEFRINLIDTPGHIDFTVE
                     VQRSLRVLDGAVVVFDGVAGVEPQSETVWHQADKFKVPRICFINKLDRMGASFVNSLA
                     SIRERLNPNAMPVQLNIGLESSFEGVIDLLQMKAFTFSGDKGEIINEIPIPQNLLAEA
                     EKYRHELVEKIVETDDALTNRYLEGEEIGIEELRKALRKATIESKIVPVFCGSALRNK
                     GVQFLLDGVVDYLPSPADLPPVKGFDPKTGTEILRQASDDEPFTALAFKIATDPFIGN
                     LAYFRVYSGTLKRGSYILNATKNQKERAGRIVRMHSNHREEVEEVFAGDIAALVGLKS
                     TTTGDTLCDERHPIILEKIEFPEPVISMRIEPKTKADQEKMSLALRKLSDEDPTFQVH
                     SDQETGETIIAGMGELHLDVLVDRLRREFKVEADTGRPQVAYKETIKSTAEAEGKYIR
                     QSGGRGQYGHVWLRVEPLERGKNFEFVDEIRSGAIPQEFIPAVEKGVREAVQKGVLAG
                     YPLQDIRVTLFDGSFHEVDSSEFAFKIAASMALQEAVKKANPILLEPIMKIQVIVPAK
                     FLGEVTGDLNSRRARIEQISDRLDSKIIDAKVPLAEMFGYVTNLRSLTEGRGFMTMEF
                     DHYEEVPKNITEQIIAGRK"
     gene            38713..41592
                     /gene="ileS"
                     /locus_tag="PL075_00575"
     CDS             38713..41592
                     /gene="ileS"
                     /locus_tag="PL075_00575"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOD96732.1"
                     /translation="MMSLLEIEKKILNFWKENKIFEKSLQKEAPKGDYVFYDGPPFAT
                     GTPHYGHLVASLMKDIVPRYQTMRGYRVERKWGWDCHGMPIENIVEEKLGLKTKKDIE
                     KIGIDKFNELCRQNVLLYVDEWRKVIERWGRWVDMDHAYRTMDLAYMESVWWVFKELW
                     DKKLIYEGYKAMHICPRCETTLSQYEVSEGYKDVIDLAVTAKFELADEPNVYILAWTT
                     TPWTLIGNVALAIGENIEYVKFRLSNHPDKLDGVYIASKNYFEKYQKFIAENPNLASV
                     KLELIEQIAASKLVGRRYQPLFDYYLNKPLEHKENLYTIQPANFVTIEDGTGVVHIAP
                     AFGEEDMALGLEKKLPFIQHVGMDGRFKEEVKDFAGLDVKPREAPTFTDEKVVDFLKK
                     KNLIFEVEPYTHSYPYCWRCYSPLLNYATSSLFVNVTALKNRLLKNAEFINWVPAHIK
                     EGRFGKWLEGARDWAISRQRYWGSVIPLWVCDQCGEKKVIGSVKELEDLTGEKIEDLH
                     KHFIDKLQFQCEKCGGTMKRIPDVLDCWFESGSMPYAQLHYPFENKEKFEKNFPAEFI
                     AEGVDQTRAWFYYLHVLATALKDKSAFKNVVVNGIVLAEDGKKMSKHLKNYTDPLEVM
                     EKYGADPMRYYLATSQVMEAEDLCFSEKGVEEVLKKVIFILLNVLSFYNLYAKQQKIL
                     LIPHFNNVLDSWIVSRLESLKEEMTEQYEQYNLAKANRPIAEFIDDLSTWYVRRSRER
                     FKNGDEEAKEVLGYVLLELAKVIAPVMPFVAEYIYKEIGGSKESVHLEDWPTVNKNLI
                     NPEVLEKMKQTRKAVEIGLALRSEHQIKIRQPLKLIQFSDEEMFNNLYEDIVKEELNV
                     KEIALGDKDWLDTEITPELEEEGMLRELTRSINQLRKEKGLKIDEKNVILEYETEDIN
                     LKNLIEKSEADLKKSCLLKTIESTTGLTTFKELEINGQKIKLNLK"
     gene            41616..>42859
                     /gene="proS"
                     /locus_tag="PL075_00580"
     CDS             41616..>42859
                     /gene="proS"
                     /locus_tag="PL075_00580"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOD96733.1"
                     /translation="MTVKEEKIIHKKITKRSEDYSQWYLDVIDAAELAEYGPVKGCMV
                     IRPYGYAIWERIQSILDQRFKETGVQNAYFPLFIPERLLEREKDHVKGFAPEVAVVTH
                     AGGKKLAEPLVIRPTSETVMYEVFARWIHSYRDLPLLINQWVNVVRWEMRPRLFLRTV
                     EFLWQEGHTVHATETEADERARLMLRIYKDFAEDIMAIPVVDGVKPDLEKFAGAQNTY
                     TVEAMMQDGKSLQFATSHNLGQNFSKVFNIKFFDKDTKEYYAWQTSWGLSTRTIGGLI
                     MTHGDDNGLVLPPKIAPIEVVLTTIETGQRDASLKQKAEEISGILKTKNIRSQVDSRD
                     LRPGEKFYEWEKKGVPLRIEVGTKEMKEDVFTVARRDTGEKIKIPFNQLANEIEKTLE
                     SIQNNLFEKALAMRQSRTKIVD"
     assembly_gap    42860..42959
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42960..>44197
                     /gene="cysS"
                     /locus_tag="PL075_00585"
     CDS             <42960..>44197
                     /gene="cysS"
                     /locus_tag="PL075_00585"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOD96734.1"
                     /translation="GHARTYIIFDVIVKYLRSRGYDVFYLQNITDVDDKIIKRAAEEK
                     VSWQKIASKYEKLFYQSMRALKVTAVSQYARATDYIPEIISQVERLLDKGYAYFLEDG
                     IYYDISKFKDYGKLAKRTRKQAEDGVSRIDDAIGKRNKGDFCLWKKSKPGEPRWNSPW
                     FFGRPGWHIEDTAIAEKYFGPQYDIHGGGRDLIFPHHEAEIAQMEAISGKKPFVKYWL
                     HTGFLTINKEKMSKSLKNFTSVDAFLKNTEDGARVLRLMIIKNHYRSPIEFNEKIVEQ
                     AKREIRSFDEFIERLKIVEDKKQKNHLKIEQFQKAFEQAMDDDFNTPKALGVIFTLIK
                     QANILINKNELSKDDIKNMMTFLGDVDKSFGFIFRKRKRKYIPLTVEALLKEREQYRQ
                     ANNFDKADELRAEIKKLGYT"
BASE COUNT        14461 a         6697 c         8923 g        14016 t
ORIGIN      
        1 aggcagggag tagctgcctt ttaagcagaa agttgcaggt tcgagtcctg ccggggggac
       61 aaaggatcta aaatttaaat tgccactctt gaattgcaaa atataataag atgaactgaa
      121 aataaaaatt aaagaattca aaatgggcgg gaattttttt aaaaaaattc ccgcccattt
      181 tagtcgctta tatattttta agcattatgc ttaatctcaa ttacatctcc atcttggacg
      241 acataatttt ttccttcagt gcgaatcaag cctttttctc ttgcttgagc aaatccgcca
      301 gcgtcaagca ggtctttcca gttgataact tcggctttga taaaagcctt ttcaaaatca
      361 gaatgtatga caccgccggc ttgaggagct tttgttcctt ttttaatagt ccaagcgcga
      421 gtttcatctg ggccagtagt aaaaaaagta attaaatcga gaatttcata ggatttttta
      481 atgagcaaat caagttcagt gtcagtgggc aatcccaatt cttttctttc ttctttagac
      541 aaattaactg cttcatattc ggttagtaga tcaataatta aataaggcca gcgattttta
      601 ttaaattcat taataatatt ttgaggaact tcttcgtctt tcccattaag aagataaagg
      661 cgcggctttg aagttaaaag ttgatattta tttaattctt ttagttcttc ttcggtaaat
      721 gattgttcaa aaagaatttt tccttcttcg agaatttttt gtgcttttga taaagcggca
      781 taatctcgca ttgcctcttt gttgcctcgt tttatatcgc cttccaaatt atttaatctt
      841 ttttcaatag tttctaaatc ttttaaaatt aattctgtat ccaaaatatc tttatcgcga
      901 aggggatcaa tgctgttttg agtattaata attttgttat tggcaaaagc gcgcaagaca
      961 tagagaatgg cgtcagtttc tctgatgtta gctaaaaatt tatttcccaa accttcgccc
     1021 tggcttgctc cttttactaa accagcgata tcaataaaat caatcgtggc atatattttc
     1081 ttttgagaat gagtgagatc cgctaaaaga tctactcttt catcaggaac agcaacaacg
     1141 ccgatattcg gatcaatagt gcaaaaaggg taattcgctt tatctacttg tttttgtgtt
     1201 aatgtttcaa aaagagtgga tttaccaacg ttgggcaatc caacgattcc aatagaaagt
     1261 ttcatagttt tttaaattaa ttaataaagt aggtttattt atttcctgta aaaatgcaat
     1321 atcgcaataa tcctttggtg gccattttat aatattcata tccggcagta gtgttgatca
     1381 aaaatttttg aagtccaaag agttttacaa accaatatgc ccaaagtgaa agtttgtgta
     1441 gtcgataaaa cgatggaaga acattttcag ttatatctcg ttcttttaca tttttgaaac
     1501 cgacactttt tataacttcg gtgaattgat cgtgccgaaa atcttttaag cccatcatag
     1561 ccgagccttc gataaccaca tctagcattt tcttttccca tggagaaaat tttttatcgg
     1621 gagcaatagt atattcaaaa agagcaaatt tacctcccgg cttcaaaatt cttaaaaatt
     1681 catttaatgt tttgcgaatg tctggagagt gcgagagggt ttcggttgta taaatcgcat
     1741 caaaataatt atccggaaaa ttggttgagg aataatccat tatctgatag tcggttttat
     1801 cttgaacgcc taattttttc gctcgctttt gtgctttctg gacttcaaat ggaacaagag
     1861 taataccaaa aacatttgat ccaatttttt ttgccaaata agttgataca atgccttgac
     1921 cgcaaccagc atctaaaaca atttgttctt cttttagttg taaattttcg gcaactaaat
     1981 cttgatataa tatttgagct tctttctcgg tgatatctgc ttttcccgtt ggatagaatc
     2041 cgaaatgttt tgaaccccaa agcacataat tgaaacccaa ttttgtttct gggttagtaa
     2101 aatactttac gactttttgt tgatttgtat tcatagcaac tctttaatag aaatattaat
     2161 ggtcttggta agttttgtcc gcagtttttt gacgttatcc taacgatatt tctgtcattg
     2221 tttttttcaa acttttccaa ttagcggtgg gtgcaggatt cgaacctgcg agaggatttc
     2281 tcctcaactg gttttcaaga ccagcgcctt aaaccgctcg gccaacccac cgaggctctt
     2341 ttattattga atttttatta taataacgca agaaaaattt tcaaatcaag ggagcagtga
     2401 gggcggcgat ttttctaaag aaaaatcgcc gcccgggttt ctccttataa ttagcgaaaa
     2461 attattcacg attttaattt ttctttaatt gggtttataa taataaataa tgactgctag
     2521 tgttttatat ataacttttt tgattattga aattattcta atgattgcag agttattttt
     2581 aattatttta tttatttctt tgttttttta tcgaattaaa acaggtgttc ctttcgtcaa
     2641 atcgaaaaaa gtacttgtcg aaaagatcaa agaagctaat atcttaaaag atgatacgac
     2701 tttttgcgat ttaggatcgg gtgatggcag ttttttgtat gaactagcaa aagaatttcc
     2761 tcatattcat ttcattggct atgaaattaa cattttttta tacatatttt ctaagttatt
     2821 caatcacttg cctaatataa aattttatca tcaggatttt tttaaagttg atttaaatca
     2881 atttgactat atttacgctt ttttgctttc tcctctgatg gaacgattgg tgcctaaaat
     2941 tcaaaaagaa gccaaaaaaa gttgctacgt tattgtgaat accttcgctt ttaaaaattt
     3001 aaaacccgta aaggagattg gttcggcgaa ccctctcgaa agtctttatt tttatcaatt
     3061 atgaaaatag ttgctgattt tcatattcat tcgcgttttt ctcgcgctac cgcacacgat
     3121 attactgtgg tcaatttaaa tcaatggatg aaaaagaaag gagtcaatct tttgggcaca
     3181 ggcgacttca ctcatcccca atggtttaag gagttgaaag aaaatttgga gccggctgag
     3241 ccgggactgt atcaattaaa aaatgattta aacgaacaaa atttcagccg ttttatttta
     3301 acgagtgaaa ttgcttgtat ttattctcaa ggaggaaaaa cacgaagaat tcatcatgtt
     3361 attttagccc catcttttga ggtggttcaa aaaattaatg tggcattgag ttggcaaggc
     3421 aatcttactt ctgatggacg gccaatttta ggcctttcaa gcattgagct tgctaaatta
     3481 cttttctcta tttcttcgga aattgtactt attccggctc atatttggac gccatggttt
     3541 tcgctttttg ggtcaatgtc tggttttgat tctttggaag aatgctttgg ggaatggtct
     3601 tcaaaaattt ttgcaatcga aactggctta tcttcagatc cacagatgaa ctggcgttta
     3661 tcgcaactgg accatttagc tattatttct aattctgact ctcattcaac ttcaaaaatt
     3721 atgcgggaag ccaacgtgtt tgatactgat ctttcgtatg aaaaaattat tgaagcgatt
     3781 aaaagcggca ccccccaaaa ttatgcacac gatccttcaa aatttcttta tacaatcgaa
     3841 ttttatcccg aagaaggaaa atatcattat gatggtcatc gattatgcaa tatagtgttt
     3901 tcacctgagg aatcaaaaaa acacaataac atttgtccca aatgcaatcg accattaact
     3961 atcggagtga tgcatcgagt agaagattta gctgaccgcg atcaaaatta tgttgctagc
     4021 gatcggccgc cttttaaaaa gttggtgccg cttgaagaaa ttattgcgga agttttaaaa
     4081 acttcaacca cttccaaaaa agttaaagcc gaatacgaga gattaattaa agtttttggg
     4141 aatgaattaa cgattttaat tgatgctact attaaagata tagaaaaaga atccagtcct
     4201 gaattagcag aggggattag acgagtaaga gaaaatcgtt taagtattgc tccgggtttt
     4261 gacggagaat atggtaagat aaaaatcttt gttgatgatg aggatattcc atcaaacagt
     4321 acactttttt aactatgttt cggttaatta aaaaatcaaa aaaatcggaa gcgcgattgg
     4381 ggcaaattaa attagcctca ggaacaatca aaacgccttg ttttatgccg attgctacgc
     4441 gaggtagcgt gaagagcgtg acaagtgctg atttagagaa aataggagct gaaattattt
     4501 tggcgaatac ttaccatctt tttcagcggc cgggagatga agtaatcaaa aaatttggtg
     4561 gtctgcatca atttatgaat tggcgcaagc caattttaac tgattctgga gggtatcagg
     4621 ttttttcttt ggctcaatta agaaaaatta gtttagcagg cgtggagttt aattcggaag
     4681 ttgatggtca aagaattttt ctttctccag aaaaagcatt agagattcaa aaaaatttaa
     4741 attcggatat tgctatgatt ttggattatt gtgttggtta tccagctaaa aaagcagaag
     4801 tagcaagggc gatgcaatta accactttat gggctgaacg cagtcgcaag tatgctgata
     4861 aaatcaaatt taaaaatcaa atttttggca tcattcaagg aggaattttt aaagatttaa
     4921 gaagccaaag tttgcaaaat ttaatgaatt tgaattttga tggttatgcg attggcggtt
     4981 tggcagtggg cgagccggag aaaaaaatga aggaaattat taagtggtta gcaccgcaaa
     5041 tgcccgcaaa taaaccgcgc tatttaatgg gtgtgggata tcccgaacaa atttttgaag
     5101 ctattcaaaa tggcgtggat atgtttgatt gcgttattcc aacgcgtcat gctcggcatg
     5161 gtgaactttt cgttcgcact aataaacaaa ttgttatttc gagacggtta aactatcaga
     5221 tcatttctat tgggaaaagc aaatatcaaa gtcagaattt gcccgttgat cgtttttgtc
     5281 attgtgaaac ttgcgcgaat tattcattag cctaccttca ccatctttat aaaaataacg
     5341 aattacttta ttatcgattg gcgacaatcc acaacttgaa gttttatttg gattttatta
     5401 gagaagttcg cttagcgatt aaaaacaatc tgatatgatt acaaaaccaa aattagttct
     5461 tcatgtttgt tgtgccgttt gtggtgctgc tttggtgaag gtattgcaag aaaaattcca
     5521 agtcattctt ttttattaca atccgaatat ttggcctcgt gaagaatacg agaagcggct
     5581 tgaagctgtt gtaaaattat cagaaattta tggtgtgatt ttaaagaaag gaaaatatga
     5641 aaataaattg tggttgaaaa aggtgaaagg gtttgagaat gaacctgagg gaggaaaacg
     5701 ctgtccgatt tgttttcaaa tgcgattaga aaaaacagct gatctggcta aaagtagtgg
     5761 ggcgttatac tttgctacaa cactcgcaat tagtccattt aaaaacgagg cagtaattaa
     5821 tgagattgcc caagaggtgg ccaaagacaa gggtttaatt tttttatcat tggcagatat
     5881 tggtgataaa aacaatttgt ggcaggaaac acggctttta gcaaaacaat atcaactata
     5941 tcatcaaaaa tattgcggtt gtcaattttc tttggcagca atgtctaaaa aacaaacctt
     6001 ggctaaaaat aagaaacatt tataattaaa ttatgaccct agatcgattt tttatatttt
     6061 atcttattgc gcttgctttt aatcgtatta agttgtttct aaaacactgg tattccaata
     6121 gttttattaa attttggaat tttttcattc aaacattgag gaatttggat cggacttttg
     6181 ctattaaaat tacggctcga cattggcttg atcctttata tcaggatcgg acagtggtgg
     6241 gttatatttt gggctttatt ttccggactt tgcggattgc catcgcggca gtgctttatt
     6301 tggcaatttt tattgtggta atgttcggct atcttatttg ggcggcaacg cctattcttc
     6361 ttattgttag agcgttcgcc taatttttaa aatatatgac cgttaaagga aaagtagaac
     6421 aattttttta tcgcgatcct caatttgaaa tgtctttttt tgagcgaatt ttagtacgct
     6481 tttttatttt tcttttttgg tcattggcgg ttgttttaac gataatgctt attttaatgc
     6541 gagaatcctc gctgcgtttt ttgggttatt tcggcataat ttttattttg gatcgcatcg
     6601 ttaattttga tcggccagac aaaaattttg ataaatcatt tgatcaaaaa ctagtaacgc
     6661 aaccaaattg taaaatcaac ttgttgccct actctgaggc gccggtaaaa agagtgttgg
     6721 ttaatgcttt tgaaaaaacc ttgtctcagg gaggtaattt taattttcaa ttattgcttg
     6781 aatttttaga agagagaaag tttcgagaga ttttcgaacg attagaaatt aattgtaatg
     6841 agttagaaag cgcaattcgg gaaaatttga agaaaaattt tacgagaccc agtaaaaaat
     6901 cattaattaa aacgattaat gatttggtag taagtgcata ttttttaaga gataaaaatt
     6961 atttacaaac aagagatctt ttcgtggcgc tattttatat caaagacgag gaattggagc
     7021 ggataaaaaa ttattttcgt attcaatctg atgatatccg tattgctgcg gtgtacagtg
     7081 aatttaagaa gcagtttggc cgttttttga cacctcgaag acgcagagaa ttggttaagc
     7141 aaacaaagca agaaaaacat aaaattatga atcgcgcttg gacggcgcgg ccgactccat
     7201 ttttggattc tgttagtgat gatctaacgg atttagcgcg ggcagaaaaa atcggttttt
     7261 taattggtca tgatggagag gtaagacagc tgattaatat tttatcgcga gctaatcgca
     7321 ataacgctct gttagtaggc gaaccatcta gtggtattgg ggctattgtt cgccacgtgg
     7381 catatttgat tgttaaagat gaagtgccat cggaattgtt tgataaaaga ttggtggcac
     7441 tttcaataga tgagttagta tcaggttgtg aacctcaagc agtggcagag cgagtgcagc
     7501 gaatcgtaaa agaaatttta gatgctggca atgttatttt gtttattccc gacatccaca
     7561 atttatttaa aacatccggc caaaactact taagcgcggc cgaggtttta ttaccaattc
     7621 tatcgcgtag tagttttcaa gtaattggtg caactacgcc gggtattttt aaaacagaga
     7681 ttgaacctcg ggaagatttt atgtcacttt ttgaggttat tcttgttaat gaattgagtg
     7741 aagaggatgc aattctgttt ttaacttatt cctcgttaat tttagagaaa acatataaaa
     7801 ttaaaattac ttacccagct attaaaaatg cggtgaaaat tgcccatcgc tattttagag
     7861 ataaaatgtt gccgacgagt gccgatgatt tattaaagga ggtattagca gatgtgcgta
     7921 atttaaaaca gaaggtttta acgggcgaag acgtgattcg tgttagccaa gaaaaaatta
     7981 atgtgccttt aaaatttgct gaaaaagagg aagcggaaaa acttttgaat ttggaaaaaa
     8041 tcatccacga acgactaatt gatcagaatg aggcagtgaa agaagtgagt aaggctttgc
     8101 gggaatatcg agccggatta tcgcgcaaag gagggccaat cgcaactttt ctttttgtgg
     8161 ggccaactgg tgttggtaag acagaattag caaagatttt agctaaaatt cagtttggtt
     8221 cggaaaataa catgattcgt tttgatatgt ctcgatatca agataaaaaa agcatttttg
     8281 atttcgtggg agcgccagaa ggtgataggc cgggagcatt aaccgaagcc gtcagacaaa
     8341 acccattttc tctcatttta ttagatgaat ttgaaaaagc gcatccggat ttaatgaata
     8401 tttttctttc cgtttttgat gatggccgct tgactgataa tttgggtcga gtaattgatt
     8461 ttaccaatac aatcattatt gctacttcaa atgctcattc ggcattgatt aaggaaagaa
     8521 ttgaaacagg ggtggcgatg gagcaaattg cggctgaatt aaaacaaaaa ttaaccgatt
     8581 attttcagcc agagttaatt aatcgttttt cagacatcat tgtttttaag cctttgtcgc
     8641 ctgatgattt aattcaaatt gctcgattaa atttggctga tttagctcaa tcgttagcaa
     8701 ctgaacaggg gattaagttg ttttttgatg acaaggccgt taaacaattg gtgaaaattg
     8761 gatatgatcc ggtttatggc gcgagaccat tgcgtaatgc aatttcggat aatgtcaaag
     8821 ctgttttagc cgaaaaaatt ttgagagggg aaattaaacg aggcgatgaa ttgatgctga
     8881 cttttgaagg tgaacaattt cttttgctcc cgcgttagcc tgttagtaga gtttttctat
     8941 tgacaaaaaa aattttttga atagtataaa gaaaatcttt ataaaggggt ggttccaact
     9001 atttaaataa tttctaattg cagtgaggcc aatttttcat aataaaaaca tctagatcat
     9061 ttaacccgtg tatagggctt tcatttcgta tcactcctct ttataaaatt tattaatttt
     9121 ttatcatttt tatgcctaat caaaaaatct tttctcactt tcctcgttct cttcaaacgt
     9181 tggaatatct tcctcatttg gcagatatcc agataaaatc ttacgagtgg tttttaaaag
     9241 aaggtctgag ggaacttttt gatgaaattt ctccggtaaa agattataca ggtaaagatt
     9301 tggaattata ttttgaagat tattattttg atgagcctaa atttaatgaa attgaaagtc
     9361 aagagaagaa tttgacttat gaagccgctc ttcggctgaa attaaaattg attaataata
     9421 aaaccaacga gaaaaaaaat caggaggtat attttggaga aattccgatg atgacaagtc
     9481 gagggacttt tattataaat ggcgtggagc gaacggttat ttctcaatta attcgttcta
     9541 gtggtttgta tttaacagca attcaaagca gagggagaaa attgtttgga gccaaaatta
     9601 ttcctaatcg aggagcttgg ttggagtttg aaactgatct tgattcagtc atttatgtta
     9661 aaattgatcg caaaagaaaa gtgccagtga cttgtttatt acgtcttttt ggattggaaa
     9721 acaaagagag tattttgaag acttttgcca atgttgatac tggtgaaatt aaatatattg
     9781 aaaaaacttt agccaaagat gttgctacca cgcgcagtga agcgctagta gaaatttata
     9841 aacgcattag accgggtgat ttagctacgc cagagaatgc gcgaggtttg attgaaggaa
     9901 tgttttttcg agaagatcga tatgatttgg gtgaggtggg aagatttaaa ttaaatcaaa
     9961 ggctcaatcg agcatccaaa gaagcagaca caaagagtcg gctgctttcg cttgatgatc
    10021 tgattgacgt ggttaaattg atcattagct ctaataataa tgaagcggct gagccagatg
    10081 atattgatca tttgagcaat cgtcgtgtta gggcggtagg cgagttatta caaaatcgat
    10141 tgcgtgttgg gattatgaga atgcgccgaa ctattcaaga tcgaatgtct acattagata
    10201 tttatagttt ggtaccggcg caattgatta atgctcggca aatgatggca atcatttggg
    10261 agttttttag tacttctcaa ctatctcaat ttttagccca agaaaatcct ttagctgaat
    10321 tagaacataa gcggcgtttg tcggcaatgg ggccgggtgg tttacaccgg aagcgagccg
    10381 gctttgaagt gcgcgatgtt catcctactc attatggaag aatttgcccc attcaaacgc
    10441 ccgaaggagc taatgtgggt ctagtaactt atttatctag ctttgcgcgt ttaaatcgtt
    10501 tgggatttat tgaaacgcct tattttcgtg tcaaaaaagg acaagtaaca gatgaagtgg
    10561 tttacctcga tgcttatgaa gaagaaaaat attatattgc tcattcaaat attaatatag
    10621 ataataaagg gaaaatcatt gacgatattg tagaagcgcg atataagggt gaaccgggat
    10681 tttatccgcg agaagatatc aattttattg atatttcacc ttttcaactt ttgagtgttg
    10741 ctacttcttt aattccattt ttggagcatg atgatgctaa tcgagctcaa atgggatcta
    10801 acatgcaaag gcaggcagtt tcgtatatgt cgagcgaagc gcctttagta ggaaccgggt
    10861 tggaaatgaa ggccgctcat gattcagggc atgttttagt aagcgatgtt gaaggcgagg
    10921 ttgttgaagt agatggcgct catatcaaaa ttaaaattaa aggaaccagt aattttaaaa
    10981 attatcgttt gcgtaatttt attcgagcta atcagtatac ttgtatttct caaagaccga
    11041 ttgtcactaa aggacaaaaa attaaaaaag gagatgtttt agctgatagt ttacaaacaa
    11101 aaaatggcaa gctcgctttg ggcaaaaatt tacttgtcgc ttttgtttcg tggggcggcg
    11161 ccaattacga agatgcggtt attttatccg agcgagtagt tcgcgacgat ttgtttacat
    11221 caattcatat tgaagatttt gtttgcgatg ttcgagaaac taaattaggg cctgagatga
    11281 ctacgctaga tattccgaat gtttctctag agaaattaaa ggacttggat gaggaaggta
    11341 ttgtaagaat tggagctgaa gtgaaaagcg gcgatatttt agtagggaaa atttctccta
    11401 aaggcgaagg tgatttaact tccgaagaaa gattattgag agctattttt ggagaaaagg
    11461 cacgtgatat taaggacact tctttgattt taaaacatgg caagcgaggt cgagttgttg
    11521 gtattagaat tttttcgcgc gagcgaggcg ataaattaga gccaggaatt attaaaagaa
    11581 ttcaaattga tgttgctcaa cgtcgattaa ttatggccgg tgataaatta gctggtcgtc
    11641 atggcaacaa aggagtaatt tcaaaaattg tgcccgagga aaatatgccc tacttagctg
    11701 acggaacacc ggtggatatt attttgaatc cactaggcgt agcttcgcgt atgaatttag
    11761 gtcaaatttt ggaaactcat ttaggttggg cggctcataa actaggatat caagcaattg
    11821 ttccggtttt tagcggcaca acagaggaag atatcaaaaa cgaatttaaa gcggctggtt
    11881 taccggaaaa tgggaaagcc acattgtatg atggtcggac tggagagccg tttagtgaac
    11941 caattatggt aggttatatt tatatgatga aactggtgca tatggtagaa gataaaattc
    12001 acatgcgatc cattgggcct tattctttga ttacgcaaca gcctttgggt ggaaaagctc
    12061 aattgggtgg tcaacgtttt ggagaaatgg aggtgtgggc acttgaagga tatggagcgg
    12121 cttatgcctt gcaagaaatg ctcacaatta aatcagatga tatcagagga agaattgctg
    12181 cttatgaggc tattgttcgt ggccaagatt ttaaaagtcc gaatatccca gcttcttttc
    12241 atgtgcttgt gagtgaatta aaaagtttag ctttaaatgt tgagttaatt ggcgttaagg
    12301 gcaaagtgca tgcattggat gagtcagagg aaggaaaatt atttgcagaa gcgatggttg
    12361 ataacaaata tctaactgaa aataatcaaa attcgaaaca aggtcaagat aatcaaaatc
    12421 cagcaaatta atatgttgcc agaagatttc tatgctatta aattaaaatt ggcttccccg
    12481 aaagaaattc tttcttggag tcatggagaa gtaacgaagc ccgaaacgat aaattatcgg
    12541 acgcaaagag cagaaaaaga tggtctcttt tctgaaagaa tttttggccc tacaaaggat
    12601 tgggaatgct attgtggtaa atataagaga attaggtata agggcgtagt ttgtgataag
    12661 tgcggcgttg aagtaacgcg ttcgagtgtc cgtcgagaaa gaatggggca tattgcttta
    12721 gcttgtcctg ttgctcatac atggttttta agaagcattc cttctcaaat agctctcttt
    12781 ttgggcgtta gccaacaaca attagaacga gtgatttatt atactgctta tatcattacc
    12841 aagattaata atgaagaaaa gaaaaagatt caggctgaat tgcaagaaga gtacaatgcc
    12901 aaaagaaaga aaacggataa gattaatatg ctggacactt tatatcatca ccatataaat
    12961 gaattgaata gccttgagcc gcttaaaatt attccagaga cagaatattt agaactcagt
    13021 aaacggtatg gtcatatttt tgaagccggc atagggagtg agccgatagt taaactatta
    13081 gagcaaatcg atttaaaaaa gaaagaaaga gaattagtta aagcctttaa aactcaaaaa
    13141 ggagataaga aaaagatcct tcttcaatta aaactagtga agtcattttt gaaaaataat
    13201 ttacgaccgg aatggatgtt tttaacaatt attccagttt tacctccgga tttaagacca
    13261 atggtagcgc ttgatggcgg acgatatgcc acttcagatt tgaatgattt gtatcgccgt
    13321 gtcatcaatc gtaataatcg attaaaaaaa ctaattgaat taaatgctcc cgaagtaatt
    13381 attcgcaacg aaaaaagaat gctccaagaa gcagtggatg ctttaattga taatggagct
    13441 cgtaaaactc aaattcaatc cagcgctcaa aagagacctc ttaaatcttt gtctgatatt
    13501 ctccagggaa aacaggggcg ttttcgacag aatcttttag gtaaacgagt agattattct
    13561 ggtcgttctg ttattgctgt tggccctgaa ttaaaacact atgaatgtgg tattcccaaa
    13621 acaatggctt tggaattatt tagacctttt gttgtaaata aacttttaga aaggggattg
    13681 gtttttaata ttcgtaatgc taaccggcta atcgatcaag gaccagccga ggtgtgggct
    13741 attttagagg aagtaatcaa ggatcgctat gtgctattga atcgagcgcc aactcttcat
    13801 cgtttgggca ttcaggcctt taaaccggtt ttaatcgaag atttagcaat tcgtattccg
    13861 ccaatggttt gcacggcttt taatgctgat tttgatggcg atcagatggc ggtccatctg
    13921 cccctaacgc aagaagcaca aaaagaagca aaagagatga tgatttcttc acgtaatttt
    13981 ttgaaaccag ccaacggcga acccattgtt aatcccacca aagatattat tttaggatgt
    14041 tattatctta ctaaaatttg ggataatcga aagggagaag gcaaaatatt ttcttcacca
    14101 gaagaagcaa tgttggctta tgaaaataaa attattgatt tgaaggcgcg gattaaaata
    14161 agaccttaca aagagcatta cttaaaacag atgggaggtg tgttggtggt agaaaaatac
    14221 gaaaatccca atactttgat tgatatttct gtaggtagaa ttatatttaa tgaaattttt
    14281 ccaaaggact ttcctcttgt aactgctgtt gtagaaaaga agtttattag tcagttaacg
    14341 gattttataa ttaaatattt tgaaagcgat aaagtaattg aagtgatgga taaaattaaa
    14401 gagataggat ttaattatgc cactcgatca ggtattactt ggtcgatgtc tgatttggtt
    14461 atacctgcag aaaaaaaaga aataatagat gaagctcgca aagaaattaa tcagattgag
    14521 caacaattta atcaagcgct gctttctaaa gaagaaaaga gagttcatgc aataaaggcg
    14581 tgggaaaata gcatttccaa aatctcaaag aatatttcaa aggttcttcc gccggataat
    14641 gccgtttttc aaatttttga ttcaggtagt cgtggttcct gggggcaatt aagtcagatg
    14701 atgggaatga aaggattagt acaaaatcct aaaggtgaaa ttatcgaatt accaatttta
    14761 tcatcttaca aagagggtct aacagtgttg gaatatttta ttgctagcca tggagctcgc
    14821 aaaggttcga gtgatacagc tttaaagacg gctaatgcag gatatctaac acgacgtttg
    14881 attgatgccg ctcaagacct catcattatt gaagaagatt gcgggactac aagtggtatt
    14941 acagtttacc gcgatgacgg agcagaatat ggtcatcatt ttagctctaa aattgtagga
    15001 cgaacaagtt tagaggatat taagataaaa aacaaattaa tagttaaagc aaacgaaatt
    15061 attaatgctg aagcggctga attaattgag gaatcaacga tccagcaaat taaaattcgt
    15121 tcaccgatta cctgtaaaac attgtgggga gtttgctcta aatgctatgg taatgatttg
    15181 gcttataata ctccggtcac attagggacg cctattggta ttgtggctgc tcaatctatc
    15241 ggagaaccag gaactcaatt aactttaagg acatttcata ccggaggcgt ggcgggacag
    15301 gatattactc atggtttgcc tcgtattgaa gaattatttg aatgtcgtat tcctcgcaat
    15361 aaggcagtta ttagcgaggt gaatggcaca gtagtgaaaa tagaggaaag ggataaagat
    15421 atagccattc aaattaagcc ggatctgatt cgaaaagaac atagcaagaa agataagaaa
    15481 gatgcgaaaa caaaagagat tgggttagaa gaatatttaa ttccgagaac taagagaatt
    15541 ttagtaaacg aaggagataa agtagtcgtt ggtcaaaaat taagtttcgg cgatattgat
    15601 ttaaaggaat tattaagagt aacggatcgt tatacggttg agcgatatat cttaaacgaa
    15661 attcagagaa tttatgtttc cgaaggtgtt tctataaaca ataaacatat tgaaattatg
    15721 attcgtcaga tgtttgataa agtaaaaatt aaaaatccag gtgatagtga ttttttggtt
    15781 ggtgatgtag tttctaagtc aagatttttg gaagaaaaca aaaaattgaa aagtaaaggc
    15841 aaaaagccag ctcgagcgat tcaattgttt ttgggtatta cgcggtcggc tttgtttagt
    15901 gaaagctttt tggcggcagc ttctttccag gaaacgcggc gcgtattagt aaaagcagcg
    15961 attgaaggaa gagtggatta tttacgtggt ctaaaggaga atgttattat aggcagatta
    16021 gtaccagtgg gaaccggtta tcgtaaacga cttgaagaaa aggaaaaaga agaaaaaagt
    16081 aaaaagaaag aaaattaaaa aatcaaccgc ttttaagcgg ttgatttttt aatatttatc
    16141 taatttctgc ggcgaggtcg acttcctggt tgattacgac gatcgtgacg ataggaggat
    16201 tgtccgcgac gagttgaatg ttgttcagag gatttttcgt gtaaaacttg attggcgcgc
    16261 gaatcagctg atgttgatgg ttggagagct ttaagcgaaa gattaatacg acctgttgtc
    16321 tcgtcaattt ttataatttt taaagttaaa atctgcccta cttttagtac atcttcaact
    16381 ttatcaattc gtcgattagc aatttctgag atgtgcagta gtccatctcg attatatcca
    16441 agatcgataa tagcaccgaa atcaagaatt cgactaacag taccagtaac gacctcacct
    16501 tctttaaatt cataagtgag gcctttaata atttctattg cttgttggcc agattcgtct
    16561 gtgggtgcgg taataaaaac ctgaccatct tcgtcaatgt caatttctac acccgtacgc
    16621 ttgataatgt cattaatagt ttttccgcct gatccaataa tgtttccgat tttatcagga
    16681 ttaacttgca atgtgaatat tttaggcgca tgaggagaga gtgagggacg aggtttttca
    16741 ataatttcgg ctattttctg aagaatttga agacgggcct ttttggctct aattaatgtt
    16801 tcttctaaaa tttgaagtgt aatgccagta attttaacat ccatttgtat tccggtaatg
    16861 ccttccgtgg tgccggctac tttaaaatcc atatcaccat aatgatcttc tggtccttgg
    16921 atatcggtaa ggattttata atgcgattca ttatcaagca tcaaacccat tgcaatgcca
    16981 gcaacaggtc ttctaatagg cactccggca tccattaaag ccaaagatga tccgcaaacc
    17041 gaagccatag aagaggatcc gttagatgat aaaatttctg aaactattct aatggcgtaa
    17101 ggaaactcat ttttattagg aataatagga tataatgatt tttccgctaa agcaccatgt
    17161 ccaatttctc ttcgcccagg gccacgcatt gaagaaattt caccaaccga ataagggggg
    17221 aaattgtaat gatgaataaa tcgtttggta tcgcatgttt cgatggtttc aatcgttaat
    17281 gcatgagaag gagcggctag agtaacaatg gataaagcct gggtggatcc tcgcataaaa
    17341 aaacctgatc catggacgcg aggaattaac ccaatttcac aataaagagg tctaatctca
    17401 tctaaagcac ggctatctgg tcttttttct ttttcaagaa tatttttatg aactaaagca
    17461 tcgatttctt cttctagaac tacaaaggct tcactgatag cattagcatc aaaattttcg
    17521 cttttcaatt tttcttccaa gttcttttta agctcgttta atttagaaac tcgtcctatc
    17581 ttaacaggtg tataaatcgc atcttctaat ttatcttgta tgaattcttt ggttttggta
    17641 cggatgtcgg gattaatttg tttcagtaca atatcagttt ttggtttagc cagttgagaa
    17701 ataatttctg attggaattt ttggagtttt tcaatttcaa tcaaagctaa ttgagtggct
    17761 tgaacaattt tttcttccgg cacttcgtcg ccgcctgctt caagcatggt aattttgtta
    17821 tcagcactgc ttaaaaagaa gtcaagttga ccgctgtttc tgtcttggta agtagggttc
    17881 actttaaatt cattattaac taaaccaatt cggatagccg agattggccc actccaagga
    17941 atgtctgagg tggcgagagc gagcgaagcg ccaataagag caggaaaatc cggatcatta
    18001 tctgcatcaa tagataaaac cgtagctacg acttgtacct ctcgtcgcat gttttgattg
    18061 aagagtggtc tgatggaacg atcaatgagg cgagccgtta agattgctcc ttcagaaggc
    18121 ctgccttcac gtcgaacaaa gcggctgcct aaaatctttc cagctgcata aaaacgttct
    18181 tcatagtcaa caaccaaagg gaagaaatca ttctcgagac gatcagtctt tcccattgtt
    18241 gctgttatga gaactgttgt ttctccgtat tgaactaaaa cggagccatt agcttgttga
    18301 gcaagagggg taaattttac agtaagaatt cgccctccta catctaatga aaaaactttt
    18361 tcatctaaca taatgaaaat tccagaaaca atttttattt agtgcagaag agaaaatttt
    18421 tagggaaaaa cactctcttc tgctctaaat tgcttctggg aaaaattttc aagaagtctt
    18481 tgaaattatc gctgaaatct tgattttgat ttttttatta agggactttt aataagaaac
    18541 ttctgaggat tttgttcgag atccacaaat tcatcagcaa tttcctttaa tttagcagaa
    18601 gcactgcgtc caaaagccac aacttcaact ggtttgccca gtgcttgaag atattcgaca
    18661 agaggaataa aatcaccgtc acctgttact aaaataatgc catctatatt tggagctagg
    18721 cgaatggcat cgacagcaag ccctacatcc caatcagctt ttttcaaacc atcgggataa
    18781 acctgcaatt ctttcattct gagttcaatg cccatccttt cgagagcttc aaaaaaagaa
    18841 gattcacctt cgacggcgtc acttttaacc acataagcaa tagctcttac caatttgcga
    18901 ttagcagtag ccgcttttaa tacttcgtaa aaatctactc gagcatgata aagatttttg
    18961 gcggaatgat aaagattctg aatgtcaaaa aaaattgcta cgcgttgatc tttatatttg
    19021 ataatcataa actaataaac tattttagat ttagagatcg ggttaacttc tcataacgag
    19081 taggagactc atttttaaga gcgttaagaa gttttttcct cttggaaacc attttcaaaa
    19141 gaccgcgacg cgaactattg tctttggaat gtttctttaa atgttcagac aatttatcaa
    19201 tctgttttgt tagtaaagca acctggactt cggttgaacc agtatctttt tcatgttggc
    19261 gatgtttact aacgattttt gatttttctt ccttctttaa catattatta aaagtaatat
    19321 atcacaataa tttgaatttt gcaagaagga atataatgtt ataataagag agaagtttat
    19381 gaatcagaaa agccttttaa atttaaaaaa tcaatatctg gaatatttgg aaatagagaa
    19441 aaacagaagt gcaaaaactt tagaaaatta taaccgatat ctaaccgttt ttttgaattt
    19501 tttaagtgag cggttgcata aaaaattaga gcaattgatc cccaaggata ttacgctaga
    19561 aaacgttcgt caatttcgac tctacttgaa ccgtactgaa ggaaccgagg gcaatcttaa
    19621 aaaggtaact caaaattatt atatcattgc gcttcgaggt tttcttaaat atttgagtaa
    19681 gatgaacgtg gaatcattgc cggccgaaca agtggaatta gctagagtaa cgcgtaacga
    19741 aattggatta attagctttg aggatttaga aagactactt aatgcgccag atgaaaattc
    19801 attgaaagga cttcgagatc gagcaattct tgagatgctt tttgcaacgg gcttgcgtgt
    19861 ttcagagttg tgtcaattaa atagagaaag cgttaattta gaaaggggtg aatttagcgt
    19921 taaagggaaa ggcggaaaga ttcgtttagt gtttctttcg gattcagcca aagcgatttt
    19981 aaaaaaatat ttagagaaaa gaactgacac tgaagagccg ctttttatta catttaaaaa
    20041 atcaaaaaac aatgaggtgc tgggtagaat aacgccacga agtgttgagc gcttgataaa
    20101 tttttatgct agaaaatcag gcattgtaaa aagagttact ccccacacgt tgcgccattt
    20161 atttgcgaca gatttattgc aaaatggagc agatctgcgt tctgttcaaa tgcttttagg
    20221 gcattccaat attagcacca cccaagttta tactcatatt accgataaag aattaaaaga
    20281 aatctatcaa acttttcatg ctcggcggcg taaaaaacct tgacaaacga taaaagatat
    20341 attagtataa ctttgttaat atggacacca aagatttaga tacaatttta aaagaattta
    20401 ttggtatttt gtcggaaaaa cagagagaag tgattgaaaa aagatatggc ttgcatggtg
    20461 aaaagccaat gactcttgaa gccattggac aagaaaaagg gttaacacgt gaacgcatcc
    20521 gtcaaattga ggaggcggct ttagaaactt tgaaaaaaaa cttaaacgaa ttgagtgatc
    20581 ttatagaaag aattaaatat cagttagaac ttttaggagg aattaggcgc gaagaacaat
    20641 tattggaaga agcctcggct ttattaactc cgaagcctgt tgagttgtgg acaagtaaag
    20701 cgatacgaga gcgatggcaa aatgatatca attttttatt agatataagt ccagactttt
    20761 tttatttctt tgaaaaacca gatatctatt ctaactggta tttaagggaa gaatttttga
    20821 aaaaagcgat taatatttat aaatttgttg aacacgaatt aaaaattaca aagagaccct
    20881 tgccgttaga tcagtatcag ttgcttttgc gtaaaacaat gaaggaattt cgaattcata
    20941 atgaaaacat tctaatttct tatttagatg tttccaaaaa atttagtttt aatccatttg
    21001 gcgaatttgg tttaatagat tgggaattaa ttaatcctaa aaatgtgaaa gcaaaagcaa
    21061 agcttgtatt gcgcaatgcc aatcgcccta tgagttatca agaaattaca aaagcgatta
    21121 atgaacttaa ttttgatggt aaacgtgcat caattaatac tcttcacaat gaattaattg
    21181 caaatgatga atttgtttta gtggggcgcg gcctttatgc tttaaaggag tggggatatc
    21241 gacctggaac tacgaaagat attattattt ctattctcgc gaaaaaaccg atgacttata
    21301 gagaggtttt agaagaggta aaaaaacagc gtttagttaa agacactaca gtattaatta
    21361 atcttcaaga caaaacaact tttaagaaat tacctgatag acgttattgc ttgattcagt
    21421 cggcaaataa gaaaaactaa tggccgaagc attagcaatt ttgcaagcgg ttttaattgc
    21481 aattgggcat ttcttaggat gggtgtggtg gattgtttta cccatcattt tttttaaagc
    21541 tgattatcgt ctttggcttg attatgttaa aaaaaagttc ctttccaagg ttgaatatgt
    21601 aatttttgag atcaaaattc ctaaatatat tgaaaaaagt tttaaatcaa tggaggatgt
    21661 ttttagcgcg cttcactcca tacagatttc taagccgaat tttaaagagc gtgttttgga
    21721 aggaaaaacg caactttgga ttagttttga aattataggg aatggcggag gcgcaggttt
    21781 ttatattcgc actcctaaag attatcggca tttggttgaa tctcaaatgt atgctcagta
    21841 ccctgatttg gagctaacag agatagatga ctatacgcag gtattttctt caatgctacc
    21901 taataaattt tgggatttgt ggggattaga ttttactttt gttaaagaag cgccttatcc
    21961 tattttaact tatccttttt ttgaagcgca aaaagaagaa aaaaccattg atcccttatc
    22021 gagtttaatg gagacgatta gtaaattaga attggatgaa aaaatttgga ttcaattatt
    22081 aataaagcca gcagatgata gttgtaaaaa agaaggagaa aaaattgtaa ataaattaat
    22141 tgggaaaaaa gagggcaaac cgcgttctta ttgggattat attttagaat tcatttataa
    22201 tttgattaaa gcgccaattc aaccgcccga gtggtccgag gataaaggga ccgaaggttc
    22261 gccaggatta atgcagcatt taacgcccgg cgaaagagag gtggttcagg cgattcaaaa
    22321 caaaatttct aaattgcttt ttgacgcaac cattcgtttt gtctacatcg atcgacaaaa
    22381 tgcatttacc cgattaaaca ttactagtac tatgggtttt ttccgtcaat ttaacacttt
    22441 aaatctcaac ggctttaaac cccacaaacc atcagtaacg aaaggaactt ggccatttaa
    22501 aaaacaaaag gagttctgga aaaaaacagt gatgtataaa aattatctta aacgtaaatt
    22561 taattccgat tgcaaagtaa ttttaaatac cgaagaattg gctaccattt atcattttcc
    22621 agttactgta gttgaagcgc cagttgttcg tcgcattgag gccaaaaaag gcgagccgcc
    22681 tattaatctg ccaatagccc agtaattttt caggaggaag gaattttaat tattggaatt
    22741 aaagtatttc cgcgtctctt gactaattaa gaattttttt gctataataa agaatagaag
    22801 tccggcagaa acggactttt atttttcaga gtcgattaaa tattattatt atgtttgatc
    22861 ttaaggaatt tttctcagcc attaatgaaa ttgctgaaga aaaaggtatt tctcaagata
    22921 aaattcttga agcaattgaa caggctgttg cagcggctta taagaaagag tatgctaaca
    22981 aaaacaatat tattcgtgct aaaattgatc cagaaacagg cgaggtgaaa ttttggcaaa
    23041 taaaaattgt tgtagatgaa agcatgctta aagaagaaga ggagggagaa gcggtggaaa
    23101 aagaagagac ttcacaatta acagcaacag agaagcagat tcagcaagga gaagagttag
    23161 caaagaaaat tcgttttaat cccgagcgac atattatgtt agaggaagca gtcaagatta
    23221 agcctgaagc taagcccggt gacgaaatca tttttccctt agagacgcac gaagagtttg
    23281 ggcgaattgc tgcccaaacc gctaaacagg ttattcttca aaaattaaga gaggcagaaa
    23341 aagaggcggt tctagaggag tttaaaaata aagaaggaga aatagtgagt ggtattattc
    23401 agcgaattga aaacagaaat atttatatta atttaggtcg tgtagatggc gtgatgtttt
    23461 ataatgaatc tattcctaat gagcgttacc gtattggcga gcgaatgaag ttttatctat
    23521 tagcggttca agatgcgggt cgagggccga tgattgtttt atcacgtagt caccccaaat
    23581 tcgtttcaaa gctatttgaa ttagaagtac cagaaattgc ttcgggcgtt gttaaaatta
    23641 tggcgattgc tcgtgaaccg ggttttcgga gtaaggtagc tgtagcatca actgttgaag
    23701 gagtggatcc tattggatct tgtgttggcc aaaaaggaac tcgaattatg gctgttttaa
    23761 atgagttggg gcaggagaag atagatatta tcaaatggtc agataatccg gaagaatttg
    23821 ttgctcaagc tttatcgccg gctaaagttt cacgagtgga aatgatgcca cgaagagaga
    23881 tgcgagtttt tgttcctgag gatcaacttt ctttagctat tggcaaggga ggacaaaatg
    23941 tgagattggc agctaaatta accggatgga aaattgatgt tcgtagcgaa tcagcgccag
    24001 aggcaaaagt ggaagggggt gtggcggagg cgttggaaga aaattttgaa gaggaaaatt
    24061 cgtcacaagt cgcagagcaa gtttcatcag aaacaaataa aacaattaat gaacaatcat
    24121 tgattaaaaa gcaactttcc gaaaaaattc aggaattact caaaagtaat ggatatgatg
    24181 aagaaagatt aaagtcggct actaaagaag agctgatgca agtcaaaggc attggcgaaa
    24241 aaacagctga aaaaattatt caaatttttc agcaataaat ttaaaattac taacttatga
    24301 aacacacgtt aaaatattta tcgcaatcac aaattgaatt agaagtttca ttaacggctg
    24361 aagaaatgga gcagttttgg caggctgctc gtaaaaaagt tttaagcgaa gttcgtttag
    24421 atggatttcg tcaaggcaat attccagagg aattaataaa gaatactgat ttagaagaaa
    24481 aaatttttga tgaggcggct aatcaagcat tcaaaaaaag cttaaaagaa gttatagatg
    24541 agttggattt ggaattgatt ggcgcgccgg aagtgaacat taagaagatt gcgcctaagg
    24601 aggaatttgt ttatgttgtt agaagtgcga ttttgcccaa gccagaacta aaaaattata
    24661 aagaaataag tcagaaaatt tttaaagaga agaaaaattt gaaagtagag aaaggagaaa
    24721 ttcaagaagc gattgaatgg cttcagaaat cgcgagctaa aattgtaccg gttgatcgac
    24781 cggctcaacc caaagatgtt gttgagatag aaatcaagtg tttagaaaac gagaaagaaa
    24841 tagcagattt tcctaaatta gaccgtttta ttcttggaga gggtagatgg ccgggagatt
    24901 ttgaaaagca attggaagac atgaaagtag gcgaagaaaa agaatttaat gttatcgtgc
    24961 caaatgatta tcataatgag aacttgcgta ataaaaactt attttttcat gtaaagctaa
    25021 atggtgtttt tgagaggcag gaacccgaat taacagatga atgggctaaa acattaggac
    25081 aattcaatga ccttaacgac ttgaagaaat ctattgaaga agggatttta gaagagaaag
    25141 aaacgaaaga gaaagaacgt ttgcgggtgg cccttttaga aaaattaatt gagcagacaa
    25201 aaattgaact acccgaaatt ttaattgaag gagaaactga aaatcaactt cattcgctaa
    25261 aacatcttat tgaaaggcag ggcatgagtt ttgaggagta tctaaataaa attaagaaaa
    25321 ccgaagatga tttaaaaaaa gatttacgca aagaagccga aaaaactctc aaaggctttg
    25381 ttgttttaag aactattgct aaattagaaa attgtatacc atctcctgaa gaaattgaaa
    25441 aaaccgtaaa cgaaattatt aattaccaag caatggaagg caatgacgtt aatcaaattg
    25501 acagagaggc tctcatggag tatactaaag aaaaattaac taacgaaaag gtctttcaat
    25561 ttttagaaaa tcaagatcaa aaataatatg gattttaaaa aaccacaatc aaaaattaaa
    25621 aatttagaag aaggatatcc tttattaatt ccgactgtta ttgaaaaatc acaatttggt
    25681 gagcgggcat atgatattta ttcgcgttta cttaaagaac gcatagtttt tttaggtgga
    25741 cctattaatg atgcggtggc aaatagcatt attgcccaac tgcttttttt ggaacacgaa
    25801 gattccaaaa aggatatcga gctttatatt aatagtccgg gtggagttgt tacttcggct
    25861 ttggctattt atgatacaat gcaatatgtg aaatccgatg tttctactat ttgtattggt
    25921 caagcagcgt cagccgctgc tgttttattg gcagctggcg ctaaaggcaa aaggtttgct
    25981 ttacctaatg cagaaatttt attgcaccaa gtaatgggtg gagcagaagg gcaggctgtt
    26041 gaaattgaaa taaccgctaa gcagatttta aagattaaaa ataaaattaa cgagatttta
    26101 tccaaacaca ccggccagcc tttagacaag attgaaaaag ataccgatcg agatttctac
    26161 ctttcagcgt ctgatgctaa agaatatggt ttaatagatg ggattattac gaaaaagaaa
    26221 aaataattag gaagtaaaca acaacaaaac tcccgcgttc gacgcgggag ttttgttttt
    26281 cggtaagcaa tttcaaaact gcggaaattt tttaaaaaat ttccgcagtt tatttcaatt
    26341 tagtttttat tttatagtgc ccagggcggg actcgaaccc gcacggccaa aaggcccacg
    26401 cccctcaaac gtgtgtgtat accaattcca ccacctgggc acgaaataaa tactagagag
    26461 aatctttttg cgagtttatt tctttttctg cattcttatc atccattttt tctttatgtt
    26521 cctcgtttac cacttcttct gtatcggtta tttcttctat ttttagttct tcaggaacta
    26581 cagatttaaa cattgacaac ttgcgcctaa ttttagttgg agaaagcttt ctgataaaat
    26641 atactttagc tttttttatt ttaggcgctt taacaatttc tattttttca attaaaggag
    26701 aatgaagtgg ccatgttttt tccaccccaa ttccgtctac cacttttcga acggtaaaag
    26761 tagcacttgg tcctttgccg tgtttttgag caataacaat tccttcaaat atttgaaagc
    26821 gttccctatc tttttctttc actttttcgt aaacgcgaac ctgcattcct gctttcaatc
    26881 gagggacgtt tttcaattga cctttaacaa tttcattgat tatatccgtc ttcatatgtg
    26941 aattagtata aaaaaataaa ataaaaagtc aaaataattt tatttgtatt tagattttat
    27001 aatagacaat atcgccatca ttaacgatat aatcttttcc ttctaccttt aattttccca
    27061 tttctcgagc tttagcccat gagccgcatt caataaaatc gcgccaattc acaatttcgg
    27121 ctttgataaa cttgtcttga aaatctttgt gaattaaacc agcagcatcc caaaccgttg
    27181 aacctcgttt taacggccaa gctcgcgttt cttctggacc attggtggtg aaaaaagtga
    27241 taagatctaa tatttcatag cctcttttga tcaagtcatc gagtttatta aggaaaggaa
    27301 ggcgattaat atttagctcg gccgcttcta tattagagag cgttgctaaa tctaattctt
    27361 tcgccaaatt aatagtaaaa atatccgttt tgttcacttg attgccaata tgatcaataa
    27421 tttcttcttt aattttttct atttcgactg gcgtaattga atcatcaaca tttaatacaa
    27481 caaggagagg cttttgtgtt aaaaagttaa agtcttttat taattctttt tctttgtccg
    27541 ttagaggaaa agtggaaatc cattgaccat tttgaagatg cctttccaac ttttgaatta
    27601 attcgaattt ttgttgggct gttttttctt ttcgcttcaa atcaccttca atttttttaa
    27661 atgcctttcc taatgcttca agatcggcaa gaattaattc ggtcgaaata atttctaaat
    27721 cacgaagagg gtcgattttt tcttcaatat gaggaatatt aggagctgaa aataatcgga
    27781 ctacttcggc aattgcagtg gtttctcgaa tataactcaa aaattgatta ccgagcccca
    27841 ttccttgagc agcgcctttt actaatccag cgatatctac aaactttact gtcgcgggaa
    27901 taacattttt tgatttttga atttcggcga ttttttgtaa tcgttcgtca atcactggcg
    27961 ctaaaccaat attagggtca atggtcgtaa aaggaaagtt ggcgatagca acttgttttt
    28021 gggtaatcgc ttgaaaaaga gttgatttgc cgatattggg taatcctaca attccaatgg
    28081 aaagcatatt ttttgatttt tagacaattt tattataaac tatatttatt gtggaaacaa
    28141 taaacaagaa aattttctta gcttacgata ttcgaggccg gcaccctaaa gaaattaatg
    28201 gtgatgtttt gtggcggtta ggatataact taccgatgat tttgcagaaa gaaaagatat
    28261 tgcctaatag aaaattaaaa ataatagttg gacgtgatag tcgacccagc ggtgcgattt
    28321 taattaaaaa tctcattcaa ggattgttac agaatgatat tgaattggta gatgcgggtg
    28381 ttattacttc tccgatgctc tattttttaa taaaaatgca tcatttcgat ctgggtttta
    28441 tcattacagg atctcatttg agcgaaagtt atacgggttt aaaaatttat actcatgaat
    28501 tgaatactct ttctggaaat tggctggtac aatttttgga tattttcaat cgaccggtta
    28561 ccgtaggttt aacaaaagga aaattgacaa ggaaaaattt tatcaatgaa tatgtccaat
    28621 tccttttgca aaaaagcaaa atcagacaat ttaaaaaaac actttctttg aaaaatattt
    28681 taatctgttg tcctaaaagc agtcagctga ttttagaaaa aattaaaaag gaattgaaac
    28741 ttaacttgca atttgttcat cggccaatcc ccaaaagatt aatagcacaa agaaaatgcg
    28801 attgcgtaat tcaatttgat tctgatgccg atcggatgca ggtgtttaat agcaagggcg
    28861 aacaaatttt aggagatgta attggtgctt tttttgtgga attgatgaaa aataagatgc
    28921 ctaaaaaaat aattcttgat tgtcgttctg gccaatttct gattaattta gcacgccgct
    28981 atcgttttaa aataatttat tcgccagcag gtcatggatt ttttaaacag gcaatgaaga
    29041 aaaacaaagc cttgttgggc attgaaaaat caggccatta ttattggaag gattttttct
    29101 ttgcggattc gggcattttt tcttttttaa tgctgcttca atttttggct ggaaaaaaag
    29161 ataagataga tgatgtggcg cgtcaatttt tccaaggcgt tatttcgccg gaaataaatt
    29221 ttaaaattaa aaaccaacga caagtcaata aaatttttca aaaaacagaa aaggcattta
    29281 gtaagaatag aattaataaa attgatggtt taacagtctt tgataaaaac tttaaattta
    29341 atttgagagt ttctcatacc gaaccaaaca ttcttcgatt aaatattgaa gctatcaatg
    29401 aaaaagtctt tcaggaaata ttgtcgaaaa ttaagcaact agtcaaataa atactatggc
    29461 cgcctcgtat cgtcttaagc agttaaacag cttgattcag cagctttttt ccgaaattct
    29521 tcaaaaagaa agtaattttt ccgaggaagt ctttgtaagt gtgagcaagg tggaagtttc
    29581 gaaggattta gcaaatgcta aggttttcat tagcgttttc cctgctgtta cggatgaaat
    29641 taaagaatat tttaaaaaga atatttttgc tcttcagcaa ttacttaata aaaaattaga
    29701 cattcaccct gtgcctaaaa ttctgattgt gtatgacgat tcggaagcca aagccgccca
    29761 cattgatgct ctattaaatc agattaaaaa ggacgctcgt cggtcaagaa aaagaaaaaa
    29821 agattgattt tatctttgaa catttacaat tgaagaaatt aagttagcca cccaaaattt
    29881 ttaagatact tgatttttat tggataatca ggtattttat tataaatgga acaaagaatt
    29941 attggtggcc acgtagccaa gtagtaaggc atcggtctgc aaaaccgata tacgtgggtg
    30001 caattcccac cgtggcctca gaagtaattt aaaaacttaa aaattaaaaa gtggaaagaa
    30061 taaaattaag aaattaaaat aaccaaaacc tcgatttttt atttttgcat ttaccactat
    30121 tgcccgggtg gcggaatcgg cagacgcgtt ggactcaaaa tccaatgacc tttaccggtc
    30181 atgagagttc gagtctctcc ccgggcacaa acgaataaaa tgagtttggg accgggagca
    30241 agccaactgc ttggcttgcg tggggacgag aagcgcgcag ctatgggcga cgagcgagtt
    30301 ggcgaggagc ccagcgagcg gcgggctgcg agcgcgagag cggaagcttg agcgacttgt
    30361 agccgagtcc caccccgggc acaaacgaat gaaatgagtt tgatccgatt ttattgtcat
    30421 cctgcttttt attgtcatcc tactttttta atgtcattct aaggacaagg tccgaatcct
    30481 ccatctctta gcaataatca gcatttactg ctgtggatga gattcttcgt ttcgctcaga
    30541 atgacaggaa aaaaacgttc agaatgataa actagtgctt gtgacatggc gatggttagc
    30601 agatttaaaa ttttaattga aaaattaaaa cgggagctaa gatcgcttcc gtttttttgt
    30661 tcaacgtttg gaaggttaaa tttttaatct ttgaatttaa attatttcca ccttccatta
    30721 gtattttatt tatcgcaata aacttgcaaa aaagaggaac agaatattaa ccctcttttt
    30781 ttatgttatg aaatttttta taataaaaat taatgaagaa agaaaagatg aaaaaggtgg
    30841 tagtcattgg aggcggaaca ggtgtgtttg tggttttgac ggctttgaaa aattttcccg
    30901 tccatctttc ggcgattatc agtatggcgg attctggtgg ttcaagtgga attttaagag
    30961 aagaattcgg tattttgccg ccaggcgatg tgcggcgcgc tttaattgct ttatccaatt
    31021 cagacaataa gcttttagcc agattattta attatcgttt taatgaaagt tattttttgg
    31081 atggtcatag ttttggtaat ttatttatta ctgctttgga gcgcattacg ggcgattttc
    31141 aaaaagcgat tcgcgaggcg gggaaaattt taaacatttc aggcgaagtt attccggtga
    31201 ctttagacaa catcaatctc gttgctcaac taaaaaacgg tcaaattatt tatggcgaaa
    31261 gcgcgattga tcgaagaaaa gagaaaaatt cttctaagat tcaaaaaatt tggattcaac
    31321 caaaagccaa aattaatcct ttggctcgcc aaacaatttt agcagctgat tatattgtta
    31381 ttggcccggg tgatttatat acaagtatta ttccaaacct tttagttagt ggtgttacga
    31441 ctgctttaaa aaaatcacgg gcgaaaaaga tttttattac taatattatg accaaaaagg
    31501 gcgaaacaga cgattttaag gcctctgatt ttattattac tcttgaaaaa tatttaggaa
    31561 aaaaaatact tgattatgtt ttaataaaca atagtcgtcc ggataaaaaa cgttttaaga
    31621 aatacaaaga ggagggtgct caatttgtag agccaaatgt agatcaatgg ggaagggcat
    31681 tggttattaa aaaagattta ttacgagaaa ggggattatt gcgacatgac cctaaaaaat
    31741 taggaacaac ccttggtgaa attattctgg ctgagaaaac gtatggaaag ctcattaaat
    31801 aagaactggt tacaaaaaat aatttctctt ccgccgccac atcaattttt gcttattact
    31861 attattttta ttggaggaat ttttttctct tcttttttaa attttcaaat agctattttt
    31921 attggattgt ttattttttt gatcagtgcg attttgggat taagcgctat tttaatgcaa
    31981 aaacgagcta ttttttttat ttaccctctt gttttcctgc taggattttt ctatctgcag
    32041 ttttataatt tttatcaaga aaatcattca agcaattatt tgaatgggac agaacaatct
    32101 caagtttcgg ccattattag cgatttgcca atttataata ataatgttag ttttcaagtt
    32161 caattattag cgccttttca aggcaaggcc ttaattaaaa cacgatctta tactacagat
    32221 tttcattatg gtgataaaat tttaatttct ggaaaatttg aacagcctca agcttatgaa
    32281 gattttgatt atcgcgctta tttagctaaa gataatgttt actctgttat ttattatcca
    32341 gaaatcaaaa ttttagcgca tcatcagggg tcttggttaa aagaaaattt gtatcgttta
    32401 aagttaagct ttcaaaaaca aatcgataaa attttttcgg agccggaaag tggatttctg
    32461 gctggcttat tattgggtga aaagagaaca atggataaag atttacaatt ggctcttcaa
    32521 agaagcggca ccactcactt aattgctttg tccggttata atatcacaat tattattcag
    32581 gctgtttctt ttcttttaat tgctttggga ttagcgcgac ctcaaacttt ttgggcggtg
    32641 gttatttttc ttattatgtt tattatgctt acgggcgctt caccgtcagt agtacgagcg
    32701 ggcattatgg gcatgattct tttgttgagt aataaattag gaaaacgtta tgaggcgcga
    32761 aatgctttat tttgcgcagc gcttctgatg attattttaa atcccaaaat tatccgtttt
    32821 gattttggtt ttcaattatc gtttgccgct accttgggct taatttatct aatgccattt
    32881 ttcggtcgtt tatttaaagt cgatcaagcc tcttttttgg attggcgcaa aattttagca
    32941 acaacccttt cagcccaaat aatggttttg cctgttttgg tattacgatt tggttcttta
    33001 cctcttattt ctcccttggc taatgtagcg attattattt ttattccagc aactatgctt
    33061 gtaggttttt tagcggtatt tctttctttt attaatctct ggttgggaca tttaatcggt
    33121 tttctaggat atcttttgct tcattttgaa attgcaatga ttaatttttt cggtcattgg
    33181 aattttgctg ccttgaattt tggggctttc cgagaaattg ttttttgggc atctgtgata
    33241 attatttttg cctttggaat ttatttaaaa aaacatgaac tcattaaaca agaaatctag
    33301 taaaatttta attattgccc ttcttattat tttttgtttt gatactcttt tgtggattga
    33361 aattttgaag gagccaaaaa atgtagaggc agatttttta gatgtagggc aaggcgatgc
    33421 gacgctttta aaatttcctt tttctggcca aattcttatt gatgctggtg atggtcagaa
    33481 aatcaaaacg gctctgtctc aagtccaggg ttattttaat aaagatatag atgtttggat
    33541 tttaagccac gccaatcttg atcattacgg aggctttctc aaattattag aaaccaaccc
    33601 tccccgaatt tttatttata atggttttga tagcgaagga gactcttttc aagcattaaa
    33661 gaaattactt cacgcaaaag aaatacccat tcttgtttta gccgcaggag ataaaattaa
    33721 aattggtaat tcgcgatttg atattatatg gccaactgct aattttttga ggaaggattt
    33781 aaatgacacc tctcttgttt tacgttttgt tgatcataat cactctatac tttttacagg
    33841 cgatgtctct gctaaaatac tttctagctt aagcaatatt caatcagaaa tattaaaagt
    33901 ttctcatcat gggtcaaaaa caggcatcac tgcttcagtg cttgatatga ttaaacctcg
    33961 aatcgcttta attggcgttg gatttaataa tcgttttgga catccttcgg ttgaagtgac
    34021 cagtttatta aaaaaaatcg gctctcaaat ttttagaacc gatttaaatg gaacaataaa
    34081 aattcatttt gatgaaaatc tttttattga aactcaataa tcaaagccat tttttaattt
    34141 taaaaacagc aagaaaaagc aaagccaaaa gaaccatcaa tccaatgaca taccagaatc
    34201 cataagggga attaacgaag ggcatatatg ccgtgttcat actaaatata gaagagatga
    34261 gcgttagagg aaaagtgata aaggccaaaa tagttagaat tttcataatt tcattcgttc
    34321 gactctcaaa aaaggtgtta ttagtatttt cgagcgattc aatcgtttct ttaaggttgt
    34381 ccagattcat ccaaagcttt tcataatctc ccagaagatc attaaaatag atttctaaat
    34441 ccttgccaaa aaattgtaca cctcgttctt ttaatgattc taaaattgaa tgttgaggcc
    34501 tggcaattaa tcgttgatct agaatgtctc ttttgacaat ggctatttgt tcaatcagtg
    34561 attttggatt acttttgaaa atggcatctt cgattttttt aatctttccg ttgatgtgtg
    34621 cgagttggcg caaggaaaaa gtaagccctt cttctaaaat atagtgaagc agatgagcgg
    34681 gggttttgtt gagaaggttt gaagggtcat tttttatttt ttcaaaaaga ttgtcaatta
    34741 atttaaagtt ttgataacgc actgaaacaa gggcatcttt tgttaaaaga aaatcaattt
    34801 cgatagcttc agaaatctga gttttttcat tgtaattaga taaataatag acgataaaca
    34861 ttaaaccatc ataaacctca attttagtgc gagttgatgg attttttagt tcagaaataa
    34921 ttaaaggatg caaatgaaat cttttttgta aatccgataa ctcttcttct gtggggttta
    34981 ctatgtcgag ccagctgatt ttagaatgaa gttctttgat catatgtata atattatact
    35041 acaggaaatt aaaaaattaa attcttacac gcctatcgac tagagatgaa gctgtttgaa
    35101 taattatagc gctgttaata ttgtttggac ttttttgatt acatttgaaa tgagaggcgg
    35161 cggaaatttt ctgaaagaaa atttccgccg cccatttcgt cgtttttttc tttttctcat
    35221 ttcggcggca cctatttttt tatggttctg acgatatgct ctatctaaat tcaccgcttt
    35281 ttcttgacat cggttttttt atttgttaaa gtatttaaca tataaaaatt aacgtatgcc
    35341 tacaattaat caattagtga aaaaaggaag aaagaaggtt gtctataaaa gcaaggcacc
    35401 ggctttgtta tggggattta attataaaaa aaatcgaccc gttaaatatc cctctccgtt
    35461 taaacgtggg gtttgtttga aggtttttac tacaacacca aagaagccga attcggcttt
    35521 gcgcaaagtg gcaagagtgc gattgaccaa tggtattgaa gttaccgcct acattccggg
    35581 tattggtcat aatttgcaag aacactccgt tgttttagtg cgaggcggtc gagtaaaaga
    35641 tttgcctggc gtgcgttatc atattgtgcg tggagttttg gatgcggtgg gtgtagaaaa
    35701 tcgtcggcag caacgttcta aatacggagc taaaaagccg caataaaatt tatgcgcaag
    35761 aaacatcgtc aaaaaagaac catcgaagaa gatctaattt ataaccgagt tgaatgtacc
    35821 cgatttatta attatctaat gcaacgcggc caaaaagcaa cggctgaaaa aaatttttat
    35881 aaagcgatgg aaatcattaa agaaaaaacc aaaaaagatc caatggaagt ttttgatgct
    35941 gccttgaaaa atgtagcgcc tatcgtagag gtaaaatctc gtcgaatcgg cggtgccaat
    36001 tatcaaattc ctcaagaagt aaaaccagat cgacagctaa ccttggcttg ccgttggatt
    36061 attcaagcag taagaagtcg aaggggcaaa ccattttatg aaaaattagc ggaagaaatt
    36121 attgccgcta gtaataatga aggagcagct attaaaaaga aacaggatac gcatcgaatt
    36181 gccgaagcta atcgtgcctt tgctcgattt tcctggtaat tattggtttt aacgaggata
    36241 aaaaaataat ctagactttg taatttaaat atttagtcgt tcaaattatt attttgagtg
    36301 cttgtttgtt tgcgagcatt ctgattctaa gatttgaatt tttaattata tgcgagaata
    36361 ttcaattgaa aaaacaaggg atattgggat tattgctcac attgacgccg gaaaaactac
    36421 ggtgagtgag cgtattttat attatactgg tatttctcat aaaattggcg aagttcatga
    36481 aggtgaagca atcatggatt ggatggagca agaaagagaa agaggaataa ctattacttc
    36541 agcggctaca acgtgttttt ggactcaaac atacaaaacg cctgaggaaa gagcaaaaaa
    36601 agaatttcgg attaatttaa ttgatacgcc agggcacatt gattttacgg ttgaggttca
    36661 gcgttcattg agggttttgg atggagcggt tgtggttttt gatggcgtag caggagtaga
    36721 gccgcaatca gaaactgttt ggcatcaagc tgataaattt aaagtgcccc gcatttgttt
    36781 tataaataaa ttagatcgaa tgggcgcaag ctttgttaat tctttagctt ctattcgcga
    36841 gcggttgaat ccgaatgcca tgccggttca attaaatatt ggtttggaat ctagttttga
    36901 aggtgttatt gatttgcttc aaatgaaagc ctttacgttt agcggagata agggggaaat
    36961 tattaacgag atacccattc ctcaaaattt attagccgaa gcagaaaaat atcgtcatga
    37021 attagtagaa aaaattgtag aaacagacga tgcgctcacc aatcgttatt tagaaggaga
    37081 ggaaattggt attgaggaac tacgaaaagc acttcgcaaa gcaacaattg aaagtaaaat
    37141 tgtaccggtt ttttgtggtt cggccttaag aaataaagga gttcaatttt tattggatgg
    37201 tgtagttgat tatttaccat cgccagctga tttgccgcca gtgaaaggat ttgatcctaa
    37261 aactggaaca gaaattcttc gtcaggcaag cgatgacgag ccatttactg ctttggcctt
    37321 taaaattgca accgatcctt ttataggaaa tcttgcttat ttccgtgttt actcagggac
    37381 gctcaagcga ggcagttata ttttaaatgc aactaaaaat caaaaagaaa gagctggtcg
    37441 aattgtccgg atgcattcaa atcatcgaga agaagttgaa gaagtttttg cgggtgatat
    37501 tgctgctctt gttggtttga aatccaccac aacaggtgat acgctttgcg atgagaggca
    37561 tcctataatt ttggagaaaa ttgaatttcc tgaacccgtt atttcaatgc gcattgaacc
    37621 gaaaaccaaa gccgatcaag aaaaaatgag tttggcttta agaaaattat ctgatgaaga
    37681 tcctactttt caggttcact ctgatcaaga aactggcgaa actattattg cgggtatggg
    37741 agaattgcat ctagatgttc tagtggatcg tttgcgacgc gaatttaaag tagaagctga
    37801 tactggaaga cctcaagtgg cttataaaga gactattaaa agcacagctg aagccgaagg
    37861 aaaatatatt cgtcaaagtg gtggtcgtgg gcaatacggt catgtttggt taagagtaga
    37921 gccattggaa cgaggtaaaa attttgaatt tgtagatgaa attcgtagtg gtgctattcc
    37981 tcaagaattt attccggctg ttgaaaaggg agtcagagaa gcagttcaga aaggtgtttt
    38041 ggcaggttat cctttgcaag atattcgcgt tacattattt gatggatctt ttcatgaagt
    38101 ggattcgtcg gagtttgctt ttaagattgc tgcttcgatg gccttgcaag aagccgtgaa
    38161 gaaagctaat ccaattttgt tagaaccaat tatgaagatt caggtaattg tgccggctaa
    38221 atttttagga gaagtgacgg gcgatttaaa ttctcggcga gctcgtatag agcaaattag
    38281 cgatcgttta gatagcaaaa ttattgatgc aaaagtacct ttagcggaaa tgtttggtta
    38341 tgtgacaaat ctacgttctt taaccgaagg acgtggcttt atgacaatgg agtttgatca
    38401 ctatgaagaa gtgccaaaaa atattacgga acaaattatt gctggccgta agtaggtttt
    38461 ctccaagata aattttgggg ccacttggag aaagccaagt ggctcgtttt ttgaaataat
    38521 caattggaaa agagaggggc agtgaaaaat ttctttgaaa tttttcactg cccctctctc
    38581 cagctcatga gttagctcat gagctaactc atgagctgga catcattcct gtctaaatta
    38641 gcagaacctt ttatattaat cgatcatctt gcaaaaacac tcccaaattt ttataataaa
    38701 aataccactt ttatgatgtc cttattagaa atcgagaaaa aaattttaaa tttttggaaa
    38761 gaaaataaaa tttttgagaa atcacttcaa aaagaagcgc caaaaggcga ctatgttttt
    38821 tatgatggtc cgccttttgc tactggcact cctcattatg gtcatcttgt agccagttta
    38881 atgaaagata tcgtgccgcg ttatcaaacg atgcgaggtt atcgtgtgga aaggaaatgg
    38941 ggttgggatt gtcatggcat gccgattgaa aatattgttg aagaaaaatt aggtttaaaa
    39001 acaaaaaagg atattgaaaa aattggcatt gataaattta atgagctttg tcgtcaaaat
    39061 gttttgcttt atgttgatga atggcgtaag gtcattgaac gttggggccg ctgggtagat
    39121 atggatcatg cttacagaac tatggatctt gcttatatgg aaagcgtctg gtgggtattt
    39181 aaggaattat gggataaaaa attaatttat gaaggttata aggcaatgca tatttgtccg
    39241 cgttgcgaaa cgacgctttc tcaatatgaa gtaagcgaag gttataaaga tgtgattgat
    39301 ttggcagtaa cagcgaaatt tgaattagcc gatgaaccaa atgtttatat tttggcgtgg
    39361 acgacgacgc cgtggacttt aattggcaat gttgctttgg caataggcga aaatattgaa
    39421 tatgtaaaat ttcgtctgtc aaatcatccg gataaattag atggagttta tattgcttct
    39481 aaaaattatt ttgaaaaata tcaaaagttc attgctgaaa atccaaattt agcttcagta
    39541 aaattagagc taatagaaca aatcgctgcg tcaaaattag taggccgacg ttatcagccg
    39601 ctatttgatt actacttaaa taagccatta gagcataaag aaaatctcta tactattcag
    39661 ccagctaatt ttgtgactat tgaagatgga acaggagttg tgcatattgc gccggctttt
    39721 ggcgaagaag atatggcttt gggattagaa aagaagttgc cgtttattca gcatgtcgga
    39781 atggatggtc gttttaaaga agaagtgaaa gactttgcag gtttggatgt aaaaccaaga
    39841 gaagcaccca cttttactga tgaaaaagtc gttgattttc taaaaaagaa aaatcttatt
    39901 tttgaagtgg aaccttatac acatagttat ccttattgct ggcgttgtta ttcaccactt
    39961 ctaaactatg ctacttcttc actttttgtt aatgttacag ctttgaaaaa ccgacttcta
    40021 aaaaatgcag aatttattaa ttgggtgccg gctcatatta aagaagggcg atttggcaaa
    40081 tggcttgaag gcgcgcggga ttgggcaatt tcacgtcaac gttattgggg ttctgtgatt
    40141 cctttatggg tttgtgatca atgtggtgaa aaaaaagtta ttggatcagt aaaagagtta
    40201 gaagatttaa caggagaaaa aatagaagac ctgcacaagc attttattga taagctgcaa
    40261 tttcaatgcg agaagtgtgg cggcacaatg aaaagaattc ccgatgtttt ggattgttgg
    40321 tttgaatctg gttcaatgcc ttacgctcaa ttgcactatc cttttgaaaa taaagaaaaa
    40381 tttgagaaaa attttcccgc tgaatttatt gctgagggcg ttgatcaaac aagggcatgg
    40441 ttttattatt tgcatgtttt agccacagct ttaaaggata agtccgcttt caaaaatgtt
    40501 gttgtaaatg gaattgtttt ggcagaagat ggtaaaaaaa tgtcaaagca tttaaaaaat
    40561 tatactgatc ccttagaagt aatggaaaaa tatggagccg atccgatgcg ttattattta
    40621 gcaacctccc aagttatgga agcagaagat ttatgctttt ctgaaaaggg tgtggaggaa
    40681 gttttaaaaa aagtaatttt tattctttta aatgttttaa gtttttacaa tctttatgca
    40741 aaacagcaaa agattttatt gataccacat ttcaataatg ttttggatag ctggattgta
    40801 tcacgacttg aatctttaaa agaagaaatg acggagcaat atgaacaata caatttggcg
    40861 aaagccaatc gccctatcgc tgaatttatt gatgatttat ctacttggta tgtaaggcga
    40921 tccagagaac gatttaagaa tggcgacgaa gaagctaaag aggttttagg atatgtgctt
    40981 ttggaattag ctaaagttat tgcgccagtg atgccttttg tagccgaata tatttataaa
    41041 gaaattggcg gcagcaaaga gtcggtccat ttagaagatt ggccaacagt aaataaaaat
    41101 cttattaatc cagaggtttt ggaaaagatg aagcaaacgc gcaaggcagt agaaattggt
    41161 ttagccttac gctcggaaca tcaaataaaa attcgccagc ccttaaaact tattcaattt
    41221 agtgacgaag aaatgtttaa taatttgtat gaggatatag taaaagaaga attaaatgtt
    41281 aaagaaatag cccttggaga taaagattgg ttagatacgg aaattacgcc tgaattagag
    41341 gaagagggca tgttgcgaga attaacgcga agtattaatc agctccgcaa agaaaagggc
    41401 ttaaaaattg atgagaaaaa tgttattcta gaatatgaga cagaagatat aaatttaaag
    41461 aatcttattg agaaatcaga ggcagattta aaaaaaagtt gcctattaaa aacaattgaa
    41521 tcaacaacag gactgacaac gtttaaagaa ttagaaatta atgggcaaaa aattaaattg
    41581 aatttaaaat agaaagatta attaaaaaaa cttttatgac tgttaaagaa gaaaaaatta
    41641 ttcataaaaa aataactaaa aggagcgaag attattcgca gtggtattta gatgtgattg
    41701 atgcagcaga attagctgag tatgggccag taaaaggttg catggtgatt cggccttatg
    41761 gttatgcaat ttgggagcgc attcaaagca ttttggatca gcgatttaaa gaaaccggtg
    41821 tgcaaaatgc ctatttccct ttatttattc ctgagcgtct tttggaaaga gaaaaggatc
    41881 atgtcaaagg atttgcgcca gaggtggcgg ttgtgactca tgccggcggc aaaaagttag
    41941 ccgaaccgtt ggtaattcgg ccgacctcag aaacagtaat gtatgaagtt tttgctcgct
    42001 ggattcattc ttatcgtgat ctccctttgc ttatcaatca atgggttaat gtagtaagat
    42061 gggaaatgcg gccgcgatta tttttaagaa cagtagaatt tttatggcag gaaggtcata
    42121 cagttcatgc tactgaaacg gaagcagatg aacgagcgcg attgatgctc cggatttata
    42181 aagattttgc agaagatatt atggccattc cagtggttga tggagtcaag ccggatttag
    42241 aaaaatttgc tggagcccaa aacacctata ctgtagaagc aatgatgcaa gatggtaaat
    42301 cattgcaatt cgctacgtca cataatttgg ggcaaaattt ttctaaagtt ttcaatatta
    42361 aattttttga taaagatact aaagaatatt atgcttggca aacaagttgg ggattaagta
    42421 cacgaacaat tggcggatta attatgactc atggcgatga taacggactg gttttgccac
    42481 cgaagattgc gcctatagag gttgttttga ctactattga aaccggtcaa cgtgatgcta
    42541 gtctcaaaca aaaagccgaa gaaatcagcg gcattttaaa aactaaaaat attcgttcgc
    42601 aggttgatag tagagatttg agaccgggcg aaaaatttta tgaatgggag aaaaaaggcg
    42661 tgcctctcag aattgaagtg ggaaccaaag aaatgaaaga ggatgttttt acagtagcac
    42721 gacgtgatac cggagaaaaa ataaaaattc cctttaatca attagccaat gaaatagaaa
    42781 aaactttaga atctattcaa aataatcttt tcgaaaaagc tttagcaatg cggcagtctc
    42841 gtactaaaat tgtagataan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    42961 ggggcacgcg cggacatata ttatttttga tgtcattgtt aaatatttgc gttctcgagg
    43021 ctatgatgta ttttatcttc aaaacattac ggatgttgat gacaaaatta taaaacgggc
    43081 agcggaagaa aaagtgtctt ggcaaaaaat tgcttcgaaa tacgaaaaac tattttacca
    43141 atcgatgcga gcgcttaaag ttactgctgt gagtcagtat gcgagagcaa cagattatat
    43201 tcctgaaatt attagccaag tagaaaggtt gctggataaa ggttatgctt attttttgga
    43261 ggatggtatt tactacgaca tttcaaagtt taaagattac ggtaaattag ctaaaagaac
    43321 aagaaagcag gctgaagatg gcgtttcgcg gattgatgat gctattggta agcgaaataa
    43381 gggagatttt tgtttatgga aaaaatcaaa accgggggaa ccgcggtgga atagcccttg
    43441 gttttttggt cggcccggct ggcacattga ggataccgcc attgcggaaa aatattttgg
    43501 cccgcaatat gatattcatg gaggtggccg tgatttaatt tttcctcatc atgaagccga
    43561 aattgcccaa atggaagcaa tttctggcaa aaaaccattt gtgaaatact ggcttcatac
    43621 cggttttttg accattaaca aggaaaaaat gtccaagtct ctcaaaaact tcactagtgt
    43681 tgatgctttt ttaaagaata cagaagatgg ggcgcgtgtt ttacgtttga tgattattaa
    43741 aaatcattat cggtcaccga ttgagtttaa tgaaaaaatt gtagaacaag ccaaaagaga
    43801 aattagatct ttcgatgagt ttattgaacg tctgaaaata gtagaagaca agaaacaaaa
    43861 aaatcatttg aaaatagaac aatttcaaaa agcatttgaa caggcgatgg atgatgattt
    43921 taatacgcct aaagcattgg gagtcatttt taccttaata aaacaggcaa atattttgat
    43981 taataaaaat gaattgagca aagacgatat caaaaatatg atgacttttt taggagacgt
    44041 tgataaaagt ttcggattta tttttagaaa aagaaaaaga aaatatattc cgttaacggt
    44101 tgaggcattg ttaaaagagc gtgagcaata tcgtcaggct aacaatttcg ataaagccga
    44161 tgaattgcgt gcggaaatta aaaaactcgg atatacg
//
LOCUS       DAOSMC010000003        41821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_1231_length_41821_cov_18.132596, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000003 DAOSMC010000000
VERSION     DAOSMC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 41821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41821
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1231_length_41821_cov_18.132596"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..88)
                     /locus_tag="PL075_00590"
     tRNA            complement(2..88)
                     /locus_tag="PL075_00590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Ser,seq:gga)
     gene            complement(100..513)
                     /locus_tag="PL075_00595"
     CDS             complement(100..513)
                     /locus_tag="PL075_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96735.1"
                     /translation="MPEIEPRIPVEKQKSDAEAAQREDLAEQVEQLRNFARWLENQQS
                     DPHSDFIHLDEMIDEELELFDKFQKGTLTEEELKIAQEKYVDELIKKMEKEGKNVKEI
                     SEVILKDGGSNFYAMMRDKLIGQKYQSKKEKEQIN"
     gene            complement(524..1432)
                     /gene="htpX"
                     /locus_tag="PL075_00600"
     CDS             complement(524..1432)
                     /gene="htpX"
                     /locus_tag="PL075_00600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOD96736.1"
                     /translation="MANLYTFKSQNIRKTWLLMGLFLIIIIAIGWFLSYYFNSPSIFY
                     YALAFSLIMNFISYFYSDKIVLGMTHAKPVTFEENPELYRIVENLCITAGLPMPKIYI
                     LPEMAPNAFATGRNPKNAAIAITEGLLQKLDRSELEGVIGHELSHIGNRDILIQTVVV
                     VLVGLISILADVFVRVSFWGGGRKNDDRGGGLLSAIIMLIGVVFIILSPLIAQLIQLA
                     ISRKREFLADASSALLTRYPEGLARALEKIATDPTPMRYTSNATAHLYIANPFKGKQS
                     QSWLVKLFMTHPPIEDRVKALREMSV"
     gene            complement(1439..1987)
                     /locus_tag="PL075_00605"
     CDS             complement(1439..1987)
                     /locus_tag="PL075_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOD96737.1"
                     /translation="MLTWIIIILVAIIILYPIFTYNAFIRLRNRVKEAASDIEVQLKR
                     RYDLIPNVIETVKGYAAHEKQVFENVTAARARAIAAEGLQEKAKAEEALSNTLKTLFA
                     VAENYPELKANANFLDLQRELADTENKIQAARRFYNSVVLDLNTKIESFPSNIIAKMF
                     KFQPAEFFEAEETEKEPIKVQF"
     gene            complement(2054..2566)
                     /locus_tag="PL075_00610"
     CDS             complement(2054..2566)
                     /locus_tag="PL075_00610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD96738.1"
                     /translation="MVKKQYRIEMIKEINQSILEDILKIETASFPEEMQSDLEDLKET
                     IQNKDGVQLIARDKKGNIVSYLSSKPLVAAYKELSDYDNGLKPEKDVLYVESIATDPA
                     ARNIEGFIEMLHALKEEAKQKGYKKISAHVRVTNGLSEIMQRRGAKKLRTIENWHDFG
                     EPFDYLELEV"
     gene            complement(2541..6386)
                     /locus_tag="PL075_00615"
     CDS             complement(2541..6386)
                     /locus_tag="PL075_00615"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOD96739.1"
                     /translation="MSQERPKINRTPEQIKEREEKREELRKILPVDLQLKFAQLQPGK
                     EPWQFEGELLKRRISKDEEDQLFYEAIKSIANKDELKLKELKELKEKKKQEIINGLKT
                     VMLYEQGFQSKYGDVRYITVRSLGALAQVVPDKAFAFYEQGFQDEDWLVRKATAESLG
                     ALAQVAPDKALPFYKRGFQSKDDHVREATAQTLGALAQVAPDKALPFYKRGFQSKDDH
                     VREATAQTLGALAQVVPDKALAFYERGFQSEDENVRKATAESLGALAQVAPDKATPFY
                     ERCFNDNNSYVRVGAGRSLEELLQVAPDKALPFYEKGFNNKDSYVRASAARSLGKLAQ
                     VAPDKALLFYEKGFNDENQYVRLVAAESLGKLAQIAPDKAIPFYERCFNNKDPDVRVE
                     AVGSLGSLAQVAPEKALAFYEQCFNDKDSDVKVVTVRSLGELAQIVPDKVISFYEKGF
                     NDKNPAVRVMAAKSLGKLAQVAPDKALAFYERGFQSEDENVRKATAESLGALAQVAPD
                     KATPFYERCFNDTDLDVRIGAAESLRELAQVAPDKATSFYERCFNDEDPFVRIKAAQS
                     LGTLAQAALDKALPFYEKGFNDKNPAVRIEAAESLWELAQVAPDKAISFYEKGFNDKD
                     WTVREATVESLRELAQVAPDKAIAFYERGLNDENSNVRLAVAGSLGELASHFNYDFFK
                     KIDAVLINDYQLKEDDLNYCYVLLANLLEHKEDPAQFIKVYLPQYRFIKSYAFKINSE
                     IRESKKWQNPEDFFKQYNYDIARLQLIDLNLSTQLLKNHLLRGLSFTESYLQVFEPVL
                     IHPALTQSIKEYLQSNQKLDGYNFSDLLEIAAAYLSLDNPQLFQDLLQKNLGKDFSEL
                     KTLLNKTLLKKLAESIQIQVEITDQDLTQWKIKYIANLITNQEFLKKESPDIQEMCDH
                     FLKSVFENRFDDFITNLNQEDEIGQAIAQHNQKVAQLFQQNGISWEDWLHFKEMVMME
                     VGTVNKMDREALFRQFENRFKAWQSDINEYEPQLKSSLAKDLAQLNQKKKEFDPSKIN
                     LDDPHWIEQLLPTYAKSLHYLQNKNPNYTLHPKALESFNHLIETIRVLTTQQQKEQTN
                     KKEFFVKLWDRDPHKDLFQGNYTHCCIAVGVKEAPPGANAYPTLHPETIFQYLIDQGI
                     NVAEIVDPDTGDVVAQTWIFVTLDENERPVLVADNFEVNNHYPAGHNVNRAIRESMFQ
                     FLNRYAESCHISKVVLGEVGTNDVETEDLKTTPLPPIQKLGGYFNDDEYYLETLGHKK
                     AWDIKRKTKEEVGKKTISY"
     gene            complement(6418..7107)
                     /gene="trmD"
                     /locus_tag="PL075_00620"
     CDS             complement(6418..7107)
                     /gene="trmD"
                     /locus_tag="PL075_00620"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOD96740.1"
                     /translation="MKVSSKKLNKQKIINFNIISLFPEAFSYFNISILKRAQKKGIIN
                     IKIINPRDFSIDKHKKVDDRPYGGGPGMVLKIDPIYKAVQFIKKRYIKSKKQKTRIIL
                     FSLKGEKFNQEMAKRLRHYENLILICGHYEGIDERIAKYIADEEISVGDYILSGGEIP
                     AMIVVDAVSRLIPGVLGKYESLEEIKGSYPVYTRPEVFYPNPKNKKIAWRVPKILLSG
                     HHQKIKEWRES"
     gene            complement(7202..7519)
                     /locus_tag="PL075_00625"
     CDS             complement(7202..7519)
                     /locus_tag="PL075_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOD96741.1"
                     /translation="MNNQIPKDQEFLEYIIKALVDYPEDVKVTRTIDEMGVLLTLKVN
                     PKDMGRIIGRQGSTVRAIRSLTRLIGLRHNARVNLKIEEPEGSSRKTDSSVDKEVESL
                     ENF"
     gene            7731..8264
                     /gene="pth"
                     /locus_tag="PL075_00630"
     CDS             7731..8264
                     /gene="pth"
                     /locus_tag="PL075_00630"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOD96742.1"
                     /translation="MILIIGLRNKGTEFQNTRHNIGAEIIMRFGENNHFQKFKLEKRL
                     QSLISKNEEIILAIPQCFMNESGIIVKKLVAYFEIPLENILVVHDDTDIRLGKFKIQK
                     NRGAAGHKGLESIIANLKSKGFWRLRIGIRSEKLISQKAEDIVLKKFSQEEQQILKKI
                     EPEIFETINQWIKEVAL"
     gene            8318..9730
                     /gene="dnaB"
                     /locus_tag="PL075_00635"
     CDS             8318..9730
                     /gene="dnaB"
                     /locus_tag="PL075_00635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOD96743.1"
                     /translation="MTPSANKEYINKKLPPQNIEAEQSILGCLMIDKEAITKVADFLT
                     PDDFYRSAHQKIYAAILDLYSKHEPIDIVSVTNRLKEKKQLNEVGGASYLASLINMVP
                     TSSHVVMYAKIVQQKRILRDLISAAYEIGEMAWGEDKDVDILLDESEQKLFQITQKSL
                     RQEFLPLKPMLTKAFERIEMLQQGGKQFSGIPTGFAELDKKLFGLQPSDLIILAARPS
                     LGKTSLALDIARHAAVKEKKSVGIFSLEMSKEQVVDRLISAESGIELWRIRTGKLNPE
                     HFEILQQGLDRLSQAPIYIDDNSSPNIMQIRTMARRLQAEKGLDLIIIDYLQLIVPRE
                     NYNGSMVQQITEISRGLKALARELQIPILAVSQLSRAVEQRDHKIPKLSDLRESGSIE
                     QDADVVLFIYRKDRDQPESDLTEEEKNTAEIIIAKHRNGPLGTIKIKFIPELTTFRNI
                     DATFGEENNEGADFGWSILE"
     gene            9735..10334
                     /gene="recR"
                     /locus_tag="PL075_00640"
     CDS             9735..10334
                     /gene="recR"
                     /locus_tag="PL075_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOD96744.1"
                     /translation="MYPDSIKNLADVLARLPSMGKRQSLRLVFYLIRNQEIRRRLLIQ
                     LNELEEKIKICEQCALPFEGAGQLCNICNNSSRAQNIIAIVEKEIDVFSIEQTNSFKG
                     TYHILGHLIDPSNINSYNTLRLDQLEKRIRRLPQQIADEIIIAISPTTEGDLTAMYLE
                     RKLKNLAKKITRIGRGIPTGGEIEFADEETLKNALSNRH"
     gene            complement(10376..10684)
                     /gene="rplU"
                     /locus_tag="PL075_00645"
     CDS             complement(10376..10684)
                     /gene="rplU"
                     /locus_tag="PL075_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007148862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOD96745.1"
                     /translation="MFAIIETGGKQYLVEEGKKIKTEKIEGKPGERVNFDRVLLIADN
                     NKVEIGKPHLKAHSVEAEVLKTGRSKKITVFRYHSKTRYRKKKGHRQWLTELKITKIK
                     "
     gene            complement(10709..11548)
                     /locus_tag="PL075_00650"
     CDS             complement(10709..11548)
                     /locus_tag="PL075_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOD96746.1"
                     /translation="MIYSELTVEEIKNLNKGHVVALFPLGAIEQHGFHLPVGTDAFIA
                     ENIAKRVEGKAKDKIVLLPTLSYGVSGGYAGSLNVDDKTFKQIVLGISQSVLDNGFRK
                     MFFLNGHGGNQSYLEDVIATLNVEYPYAATIPLYLSGKRGIQALKKVNYKYWIRHADE
                     IETSLMMAMNEKLVSKDKIVDEVGLLKTKDYKPLDEGTLKLFLPWEYESKSGVYGEPS
                     KASKEIGEFLWEEAAEEILDIINQFEKITQKIEKFISQREAENNHQSTDDNKEANKSQ
                     LTN"
     gene            complement(11583..12308)
                     /locus_tag="PL075_00655"
     CDS             complement(11583..12308)
                     /locus_tag="PL075_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD96747.1"
                     /translation="MKFSIVIPCYNEEKTLPFLLESLRQLDYPSREFEVIIVDNNCTD
                     QTVAVAQKWGVNKIVKEERQGLTWARQKGFLAASGEIIVCLDADCRVPSDWLKKAEKY
                     LQKSDVIAASGPYDYFDAPVFFRRTSLLFQSIFYPIIPPFLHFIFRKPAAIIIGGNCF
                     IKKSILEKIGGFDTSVKFWGEDVMTALRLAKEGRVVFSSHLKVLSSNRRFQKEGSIKT
                     SLRYFLNYLSIYFYNKPYVREKD"
     gene            complement(12318..12389)
                     /locus_tag="PL075_00660"
     tRNA            complement(12318..12389)
                     /locus_tag="PL075_00660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12355..12357),aa:Gly,seq:tcc)
     gene            complement(12415..13011)
                     /locus_tag="PL075_00665"
     CDS             complement(12415..13011)
                     /locus_tag="PL075_00665"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOD96748.1"
                     /translation="MKKKNKKSKQFFIVGIDEVGRGAIAGPLVLGAAAGYLNNKNKKF
                     LIGIKDSKKLTFNQRFLWHEKFKDSDLNFYCVFISNNFIDKYGMGKALKEGVRRLLKK
                     IDKKIDLVLLDGGLKAPEKYNQKAIIQGDDKIPFISAASIYAKVKRDLYMIKMDKKIN
                     YCFADHKGYGTKKHFSLIKKNGISKIHRKTFIKNIVKK"
     gene            complement(13362..13808)
                     /locus_tag="PL075_00670"
     CDS             complement(13362..13808)
                     /locus_tag="PL075_00670"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOD96749.1"
                     /translation="MKKNANEKIIAYTDGGSRGNPGPAAIGVVVEGVSHEPKQYNEFI
                     GETTNNEAEYQAIIFALRKIRQLIGRERLKQIPVEIRLDSELIAKQLNGEYKIEDKKL
                     QPLFMTIWNLKFDFGKLSFRYIPREQNRLADKLVNLCLDQQNSRLF"
     gene            complement(13810..15531)
                     /gene="recJ"
                     /locus_tag="PL075_00675"
     CDS             complement(13810..15531)
                     /gene="recJ"
                     /locus_tag="PL075_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOD96750.1"
                     /translation="MKEIQWKIRESMPQEFAKSFPEFNRPLLQLFWNRNLRTKESIYK
                     FLNPALHHLYHPKEMLNMERAVQRINEAIKNQERIAIYGDYDTDGVCSTSILAKTFKI
                     LNYHNFDVYIPNRFRGGYGLTEEKIKKMHQRKTNLIITLDCGITDIEEIALAKRLGME
                     VIIFDHHLVPDKLPDTLIVDPWQKNDPYPFKDLVASGISYKLAIFLLETNRHPLKPIL
                     EKQFLDLVAIATIADVAPLIDENRIFVSEGLKQLVKTQNIGLRKLLEIVGLNPKTKLD
                     PYHVGFIIAPRLNAVGRVEHNIEDNIEETDYSFELLMTENENEAEFLARKVNDLNLER
                     QNKVQEIYDSIKKELSQQPKLDKIICLGNPNWSKGVIGIVAGKLKDEFNRPVFIYSEG
                     KETSTGSARSINGFNLVELIGRHQDLFIEAGGHRLAAGFSLKNENLKRLKEILQEEGS
                     NIKDVDLIPKLIIDTSLDADEITSQFLENFYRFEPFGRENPEPIFLMSDVVISDIRIV
                     GNSGKHIKFSIQKKNRYFNVISFNNAEQLKFLKSGSEADIVFSLRTEEWGGQQFLEFQ
                     LLGLKEK"
     gene            complement(15533..16693)
                     /locus_tag="PL075_00680"
     CDS             complement(15533..16693)
                     /locus_tag="PL075_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOD96751.1"
                     /translation="MIKTITPKAISHKKILLRVDFNVDLDDKGKILSDFRIRANLPTI
                     EFLLKHQAEKIIIISHLGRPKGKDKKLSLLPIAKHLEQLLKQRVVFLQDCIGAQIKNE
                     INIRKERIFVLENLRFYEEEENNDKKFAQQLAALADVYINDAFGVSHRLNASVAAITQ
                     FLPSYAGLLLTNEVNNLNRLIKNAISPFVVVLGGAKISTKLPLIDVFLEKADTILLGG
                     GLANTIWKAWGFEIGKSLFEAEKISDAKNLGSRKAELVVPADFLVASSFKSTKGKVKE
                     IGDIGPKDIIVDVGPIATKAFCGLIKSAKTILWNGPMGYWENKAFRNSTIKIAQAIAN
                     NQGFTVVGGGETLTVIEELHLLNRYSFVSTGGGAMLEFLAKGELPALNFLKK"
     gene            complement(16690..17457)
                     /locus_tag="PL075_00685"
     CDS             complement(16690..17457)
                     /locus_tag="PL075_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOD96752.1"
                     /translation="MAKINPSSFWQYTLGIFLIIISIVALIAGWGVSHVISNMPPEHT
                     LTVSGEGKIFAKPDVAIVEMSVISQHKNVELVQSENDQKMQQVVNFLKESGIDEKDIK
                     TTQYNLQPEYDYSWCRTTEYPVYCPPKLVDYMLTQSLQVKIRDLSRVGKIIGNLSEIG
                     VNQISDISFVIDNDTEIKAAARQKAIAEAKQKAQEMANAANIELGKIIEINESVSGLT
                     PQRIVYQKTIESADATAPIEIGTNEIVVSVSLIYEIK"
     gene            complement(17466..18272)
                     /gene="tpiA"
                     /locus_tag="PL075_00690"
     CDS             complement(17466..18272)
                     /gene="tpiA"
                     /locus_tag="PL075_00690"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOD96753.1"
                     /translation="MKTIVANFKMNPATSEEAKQLLTLYKEESKKNQNVNLIIAPPFL
                     YLETAKNLNLQLAAQNCFYEDQGAFTGEISPLMLKNFGVQYVILGHSERRQLGETNEI
                     INKKVRAVIQNNLTPILCIGETQQERQAGETEVVLKNQLEQALSSNTNYFKQLDSLFI
                     AYEPVWAIGTGDFCDPIEVSQAMKFIQNTINAIFAYQSESSDNRNSQSTVESAAQTIM
                     PNLTLIYGGSVDSKNINDFLKQVEINGVLVGGASIKKEELVNVITIANSY"
     gene            complement(18482..19426)
                     /locus_tag="PL075_00695"
     CDS             complement(18482..19426)
                     /locus_tag="PL075_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOD96754.1"
                     /translation="MDEVKKHQLIILGAGPAGTAAGVYAARKKIDTLIITENFGGQAV
                     VAAKIENIVGFEEISGIEMARRFEKQLRHYTELEIIIDRVNGVVFKEGIFEIKTDHGT
                     FYGERILATLGRHYRQLGVPKEKELEGKGVFYCSTCDAPLMRNKRVAVIGGGNSALLS
                     VIDLIPFASQIYLLTNESDLRCDPVLIDRIKHDPKIEIHYNVEIKSIEGDKMVSGLKY
                     KEGNDLREHSLAVSGIFIQAGMQPNSELFKNLLKMNEAEEIIVEPLSGRTSHRKVWAA
                     GDITNLPYKQISVAIGDGVRALLDIYNEIRNTELKEFI"
     gene            complement(19444..21855)
                     /locus_tag="PL075_00700"
     CDS             complement(19444..21855)
                     /locus_tag="PL075_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HOD96755.1"
                     /translation="MINSLLTPLNIIALIVAIGLVMFLILEMRRRNIFSSLLKKSFKY
                     NLFLIKIPPEEQPSEKNQTVKEKINIAEQLISILANFKKPIVFEIALPEIGKEIAYYA
                     AVAQDLEESFKKHLLSFYPKAYVEKVRDYNIFNPNGFSTGVYLKQGENWMLPIKTYTE
                     LEADPINPILNAFKLKEEGEGMVVQIIISPTPKSFKTQVTKAIKHLKEGQSFKDVLGK
                     YSFGDFAKEFVSSVSVKDKSKEEPKPKVLDENAIKLLEAKVSKPLCAVNVRILVSAET
                     KERTDEALNILLSTFDQFAAPLKNNFKAVKPSNFNKFVFNYSFRLFNNKEAMYLNTEE
                     IASIWHFPLTVEVAPQLKWVKAREAPPPIELPDEGIVLGEAVYQNQKKTVRILPNDRR
                     RHVYTVGQTGVGKSVFLINMAAQDIEEGRGVCFVDPHGDVIETLLGLVPQERFKDVVV
                     FDPSDLEMPMGLNMLEYDISKPEQKTFIINDLIDIFDKLYDLSQVGGPMFEYYLRNAV
                     GLLMSDTTETPTLIDVPRIFTNPDFLKKKLANSNDPMIVEFWEKEALKVSGSSDLSLQ
                     NISPYITSKFNTFITNDYMRPIIAQRHSSLNFREIMDGQKILLVNLSKGKIGELNASL
                     LGLIIVGKLLMAAFSRVDTPEEERKDFYLYIDEFQNFTTPSIATILSEARKYRLDLTI
                     SHQFIAQLQDKIREAVFGNVGSMVVFRVGVKDAEFLVKEFSPVFNENDMINLDNFTAI
                     VKLMVNNKTTRPFTMRTIPPLKGDTGKAKVLKELSRVTYGRPRALIEQEIKERFISEE
                     ISEEL"
     gene            complement(21860..22252)
                     /locus_tag="PL075_00705"
     CDS             complement(21860..22252)
                     /locus_tag="PL075_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96756.1"
                     /translation="MAEFKTEQPNFNPQQLEEISQKVQERLGEQKEVLEKREVVREVV
                     EKKINQAMPTTQTATPITDDGVSLKLSEIKKIPEEERIKAIVRVALQDSIADAVKIAQ
                     KLGPYYVDGLHDTLVDEFLNILIQQKKI"
     gene            22549..24495
                     /locus_tag="PL075_00710"
     CDS             22549..24495
                     /locus_tag="PL075_00710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOD96757.1"
                     /translation="MFPNIISIAQKIVHKKIASHFIIFVIIIAGLLMLHSSFQESATM
                     DELAHIPAGYSYLKFRDNRLNPEHPPLLKDLSAIPLLFLNLKFPTDSPYWQEGVNSQW
                     DIGRVFLYESGNNADQIIQWARIFPILLTLLTTVLVYFLAKKIVGPYWALLPFVLFAF
                     SPLVLAHGHYVTTDLAAAFGALIIFLFVIPKLIQPSPKNIILAGIALGIAQLTKFSLL
                     IFYPLIFLFLIIWEIIKLIRQNRNHESSPVIKQLLKTIGNLFAVFAISIIVIYLVYLP
                     QNINYPQEKQTSDTEQILNSFAGGPDKELVTCRQWVGSLNRQMRCLAELDIKMTQNKF
                     TKPLAQYALGALMVVKRSSGGNTGYFLGNVSATGWHSYFPIVFLIKEPIPSLVLMFFA
                     LFLAIKNFFSATTNNIKSKIWWQKLIDWLSHNFLLFSILIFVIVYWISSISSPLNIGY
                     RHLLIITAPMFILTTDQIKKWWWGIKNTPSFKLLLFKIMTSFDQLKKIGAKSAFIAII
                     LIWYLVETIVIAPHFIAYFNEFVGGAKNGYKYTVDSNLDWGQDLKRLAKFVEENHIEK
                     IKVDYFGGGSPAYYLGDKFEPWQSAKGRPEPNSWLAVSLTFLQNSQGKPVPGFERKPE
                     DSYEWLKNKTPDAQIGYSIFVYHF"
     gene            complement(24742..25332)
                     /locus_tag="PL075_00715"
     CDS             complement(24742..25332)
                     /locus_tag="PL075_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018033870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOD96758.1"
                     /translation="MKNPQEEKTFVLIKPDGVKRGLVGEIVSRIEQRGLKIVGMKMIW
                     ATKDQIDEHYPKNDAWIKRLGEKTLATYEKYGYDTKEEVGTDDPMEIGKIVRGWLMDY
                     MTSGPMVKMIVQGIHAVDMVRKMVGNSTPALAEMGTIRGDFSVDSSILANRGKRAIHN
                     LIHASETTEEAEHEISFWFAPEELCEYKRAEEDIMF"
     gene            complement(25338..26873)
                     /locus_tag="PL075_00720"
     CDS             complement(25338..26873)
                     /locus_tag="PL075_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96759.1"
                     /translation="MKISPPSKIHKHLIAKEYIGGLEITDTALHFLLFDPYKTDKIIL
                     SFESDLEPGTVIKGELKKPEALKEVLLNLRRQPALKKIKNLYTILSLESNIVYYKIFD
                     LPTVDRNSILSAVELNTRMLSPIPFDRVYSDWELIDKPIKSDSFKLRVFSVFAEKTIV
                     DAYVKVLDEVGIMPLAVEFNGLSLLRFFSHYNIFDNLEKNYLGIYISANGLEFILGSK
                     NSLEFNFFQTWQDAFNVLGLDSSELQKGIISKENFIKIFDDNLQKVITFYFTRFQESI
                     EKVILVTPLHFDDLSNLIRDRYNLNVENPISKIKDITPGYYFATGAALRGLIPRVDDV
                     GVSLMAVGTEEEYRRSRTTNFLGLWLKIIVEVGVVLLLISIGLNIFLSYSKTNVMKDK
                     ELLSIQFDRAQLEKLSQQSKEFNANIAQAIEAKKFVKDWAPVFEAWQQAAENIHFKSL
                     IIPGAENNFSFNGWTTSQSEMIKFRDALLESGYFTDLDIPLQSIIQQKNKVEFNLKGK
                     IKL"
     gene            complement(26900..27307)
                     /locus_tag="PL075_00725"
     CDS             complement(26900..27307)
                     /locus_tag="PL075_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96760.1"
                     /translation="MKKFFEFQKRKGFSLLPVVLIAGIIVAEVGVALSFVMYLTNSAS
                     YGARLSQEAFYGARSGVNDAFLKIIRNKDYSSPGYNLTVGKSNVNVVVTNEIGIATIV
                     STGTVLNRQRKLQAVISIDAETGEAALFSLQEI"
     gene            complement(27307..28308)
                     /locus_tag="PL075_00730"
     CDS             complement(27307..28308)
                     /locus_tag="PL075_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96761.1"
                     /translation="MKSWITKDDVKKEFYWPERGQVLAEILVAVMLAGIIIGGIAATI
                     GTSVITGNKVRETTVAVGLIQDAMEGIKAISESSWVSFYCLPNGICPGSKGSDNHFYP
                     DYVDGSWQIVSGEKLKIVEGVDYTYYFYVENVNRDSSGNITTTGGTEDPSTQKITIVV
                     NWPPNNSVVMSEYRTRVTSNYFADYNWDVGRISSGVFVKSAGYYASTNGPISIINGTI
                     TLSTSTDGFLTSPVFDTTFAEGVAFNGIRWKGALPTGAHVRFQLATSNTSTPATWNFI
                     GPDGTTSSYYEAGMDQIASITLNNHNNQRYFRYIVYLYPEPGGAVPVVSKVIITYSP"
     gene            complement(28339..28827)
                     /locus_tag="PL075_00735"
     CDS             complement(28339..28827)
                     /locus_tag="PL075_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD96762.1"
                     /translation="MKLLFSNNSRGFTLIELIISLVIIAMIAVISFANLSGHREELSV
                     DLEAEQMVAYLREARNRALAGQDDSSWGVHFVNVASDSDYYNLFRGLTFSTSAIVDTH
                     FLSKQVEFVVPPVNSSSDIVFLRSTGNLSNSATFTVVIRSVIEPKYSSTIEVNRIGQI
                     NY"
     gene            complement(28834..30051)
                     /locus_tag="PL075_00740"
     CDS             complement(28834..30051)
                     /locus_tag="PL075_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOD96763.1"
                     /translation="MLFKYRASTKDGKIIEGEGDFINEDAVLEYLKSQDLNPINLELV
                     ATKSKLKTRLFGSPININDQIFLTRDLALMLKVGTDIFKAIDILIADYEKPAVRSLLI
                     EIRENLSKGQPFYTTFAKYPKIFSSVFTNLIRAGEASGNLETVFEELSVTLEKQRDLQ
                     QRIKSALIYPILLVVASIGIIALLVTFAIPKMATLFQGGDMPLPPITKIIFDVSFFLS
                     NYGLYLLIGLVLLIVAAVMFYKMSLTGRAFFQRIAFRLPVIKTVLSKMAYQRFATTFG
                     TLMRAGLPILENLEITATTVGNEEMRQALLRIANEGVAKGISLGDAFRKEEIFPHTIR
                     TLISIGEKAGRTDEVLKTLADFYDTEIDAAVKSLVAIFEPIMLLFIGVIVGGIALAVI
                     LPVYQFTNQLGGM"
     gene            complement(30067..31776)
                     /locus_tag="PL075_00745"
     CDS             complement(30067..31776)
                     /locus_tag="PL075_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOD96764.1"
                     /translation="MPLRLPIDKLKEFLVDTGLVSLESFQLAQEDAQRTGQDLVDILI
                     SRGFISEDYVYQFYSDYFKTPFIKLRGRLIKKSILSLLPEETARLRRVAVFDKDDKGF
                     VYLAMEDPGDLETINFVQKYVNAPLKVFLTTAKDLNYVYSLYGKEVVEDFKKVIEDNL
                     KETARLKMMGEKEVAEEIPIVNIVDAILAYAISLNASDIHIEVLEDVVLIRFRIDGVL
                     HEMLRIPKEVQPAIVARIKILAGLKIDEHRKPQDGRLRYEALGEAMDIRVSIMPTFYG
                     EKVEMRLLRATAKPMSLRELGMLEDTVLIVEDSIKKTYGMLLVTGPTGSGKTTTLYSI
                     LNMLNKPDVNIVTIEDPIEYYIRYVNQTQINPQAGITFATGLRAILRQDPNIIMIGEI
                     RDEETAEIAVHSALTGHLVLSSLHTNDAPTAVPRLIDMNVPAFLVAAVLNTVIAQRLV
                     RRICKDCIVSYNPEPEMIESLKRQVKDIGGEEIEVPKILYRGAGCPICNYSGYRGQVG
                     IFEVLNLDEDLRRLVNKPDFSLDVLKNLAKQKGMITMLEDGIRKAQLGITTIEEVLRV
                     IRE"
     gene            complement(31782..32390)
                     /locus_tag="PL075_00750"
     CDS             complement(31782..32390)
                     /locus_tag="PL075_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96765.1"
                     /translation="MLFLSQTVTFLPAIYFLITVGLIIAFKKIKQFIKPAVILILVLK
                     VGEMVVKIINQYFVFKNSDVGPFLLPPHQQLNQFFFHYIDYRIIYPTLFPILIGLLFF
                     FLTKIANRLSGERFFEKEEPWMFFYAIMLVGHPLWLVYIFAIFIIGLILYFLRLIFKK
                     INFGERLPLYYLWLPLAIILFLFQGTYATLPIIGDFLIKISF"
     gene            complement(32428..32976)
                     /locus_tag="PL075_00755"
     CDS             complement(32428..32976)
                     /locus_tag="PL075_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOD96766.1"
                     /translation="MKKGFTLIELLVVIAILAVLMSVVVVTINPAEMLKKTRDTKRIS
                     DLDALRTALNLYLTDQQLFTDTSCRASQVGAPTIGTCTSSIGSTSQSPSGNWTSIDFT
                     KISSGSPLSTIPLDPKNATTSVNTTTYAYYFKSNAASSTFTLLCNMESSYYSNGAGTT
                     DVESKDGGNQADFYEVGSAMLF"
     gene            complement(33064..33639)
                     /locus_tag="PL075_00760"
     CDS             complement(33064..33639)
                     /locus_tag="PL075_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOD96767.1"
                     /translation="MVNPKNYFSQPTKGFTLIELLVVIAILAVLMSVVVITINPAEML
                     KKSRDSRRVSDLDALRTALNLYLSEDKDFTSLDNTKAYLSLSGSPCTVASYGGKTATQ
                     CVADPRKVDGNGWIPLNFTSLSIRSPLSSLPVDPTNSNPYFYAFTVSSTKTMYELNAK
                     LEANSNSGLMSSDGGNSATVYEIGSDLTLFN"
     gene            complement(33671..34072)
                     /locus_tag="PL075_00765"
     CDS             complement(33671..34072)
                     /locus_tag="PL075_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOD96768.1"
                     /translation="MENQNNTNNYTSKILVIEDDPFLANLLKLRLEKDKFEVIQAKDG
                     EEALKKLKEIEPDLILLDLILPKKSGFEVLEDISLNPSLRNKPVIILSNLGQTADIQR
                     GKQLGAVEYFVKAKISIDDLILKIKEFLQKK"
     gene            complement(34261..35853)
                     /locus_tag="PL075_00770"
     rRNA            complement(34261..35853)
                     /locus_tag="PL075_00770"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(36257..36712)
                     /gene="rpsI"
                     /locus_tag="PL075_00775"
     CDS             complement(36257..36712)
                     /gene="rpsI"
                     /locus_tag="PL075_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOD96769.1"
                     /translation="MGAAEELKEEKLEKEEKENDKKTKVYYEGVGSRKTATARVRLYT
                     KNKDIIINGKDYKVYFPLKKWQATVEAPFEKMKCNGKFGLTAHVVGGGLSAQAEAVRL
                     GISRALVSFNPDFKKRLRRAGYLTRDARMVERKKYGLKKARRAPQWSKR"
     gene            complement(36822..37166)
                     /gene="rplM"
                     /locus_tag="PL075_00780"
     CDS             complement(36822..37166)
                     /gene="rplM"
                     /locus_tag="PL075_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOD96770.1"
                     /translation="MREYKIDATNKSLGHLASEISLILQGKDEPDYQPNKVADVKVIV
                     ENVSKMKLTGKKLEQKIYYHHTGYSGHLKKKSMKNIFEKNPTEVLKKAILGMLPKNSL
                     RSKRIKNLKLYA"
     gene            complement(37171..37524)
                     /gene="rplQ"
                     /locus_tag="PL075_00785"
     CDS             complement(37171..37524)
                     /gene="rplQ"
                     /locus_tag="PL075_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOD96771.1"
                     /translation="MRHLNKGRKFGRTRGDRKLFMKSLINNLIINKKIETTEARAREL
                     KVQTEKLVTLAKKQTLSAYRLLISRIGEKSAKELFYNIAPQLKNRKGGYIRIVKSPRR
                     RKRDGAPVAVVEFVL"
     gene            complement(37527..38315)
                     /locus_tag="PL075_00790"
     CDS             complement(37527..38315)
                     /locus_tag="PL075_00790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOD96772.1"
                     /translation="MTGFTFPNKPKIIKETETEGVFEIENLYTGYGITIGNALRRVLL
                     SSLKGFAITTVQIKGVPHEFSVIDGVLEDVIQIILNLKKIRVKCFSEGPHTLILKAKG
                     EGEVKARDIEKNSNVEILTPDTHIATLTDKKAELELILTVEEGVGYVPAEARQKEKLP
                     IGTISLDATFTPIKKVNFEVENMRVGERTDYNRLRIFIETDGSILPRMALKQAANILE
                     EHYRIISDIPLLEEKEPKEKEVVKKKTKEKETKKKPAKSKKTKK"
     gene            complement(38323..38946)
                     /gene="rpsD"
                     /locus_tag="PL075_00795"
     CDS             complement(38323..38946)
                     /gene="rpsD"
                     /locus_tag="PL075_00795"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOD96773.1"
                     /translation="MALDAQCRKCRRAGEKLFLKGERCYSIKCALIRKPYAPGMHKKR
                     KAKTASEYGRQLNEMRKVKLTYGVNEAQMRKYFREATKSPESTSDTFFQKLELRLDNV
                     IFRLGFAPSRQAARQMVSHGHILVNNKKIDVPAYELKKGDVITIRPQSLSKVPIKNLS
                     KNLKKVQCPDYLSLNPEKLEAKVVAEPSLAKQMPPFDFRQIVEFYSH"
     gene            complement(38958..39416)
                     /gene="rpsK"
                     /locus_tag="PL075_00800"
     CDS             complement(38958..39416)
                     /gene="rpsK"
                     /locus_tag="PL075_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOD96774.1"
                     /translation="MGKKRVIVQNEEEVLKEKEVLESAMKKTEAKEIEKKGAYTSAKI
                     YITSSYNNTLITVADKEGNVLAWSSAGHLQFKGPKKATSFAAAKVAESIAQKIKKLHI
                     SEFEVYLKGLGAGREAALRSLIAQEFNIVSIKDITPVPHGGCRPPKVRRV"
     gene            complement(39418..39804)
                     /gene="rpsM"
                     /locus_tag="PL075_00805"
     CDS             complement(39418..39804)
                     /gene="rpsM"
                     /locus_tag="PL075_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOD96775.1"
                     /translation="MVRICNVNIPDNKSIYISLSYIYGIGQSLAKKILKTANIDIQKK
                     TKDLTAMEISRLQEIIDNNYKIEGDLRREIQSNVRRLKDINAYRGIRHARKLPVRGQR
                     TKTNSRTVRGNVRRTVGSGKRKLELK"
     gene            complement(39808..39924)
                     /gene="rpmJ"
                     /locus_tag="PL075_00810"
     CDS             complement(39808..39924)
                     /gene="rpmJ"
                     /locus_tag="PL075_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOD96776.1"
                     /translation="MKVRASVKKICRDCKVVKRKNRVFIICKKNPKHKQRQG"
     gene            complement(39929..40135)
                     /gene="infA"
                     /locus_tag="PL075_00815"
     CDS             complement(39929..40135)
                     /gene="infA"
                     /locus_tag="PL075_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOD96777.1"
                     /translation="MKQNQYEGVVIEALPSTTFKVRLDDGREILAHLAGKLRLNFIRI
                     LVGDRVVVEMSPYDDKRGRIVYRK"
     gene            complement(40214..>41821)
                     /locus_tag="PL075_00820"
     CDS             complement(40214..>41821)
                     /locus_tag="PL075_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96778.1"
                     /translation="VILIVPENARISESILNFRLIKREADAANKKIYINSDAEEVSLM
                     AKESGIKIAELSQISRPSIKNKILIGDIRPPQINKISKSKKMTESVFDGVEMQEGSKN
                     KTKPDEEINPPKEKKFFTDYFDKETFSEIEISEKELRKARKKEHKKIHFGKGVWITLG
                     IILLFFIASYLFINFLAKAEVDIISKKYEWEEQLPVLATSNIQEVDYNNFQIPLFYFE
                     SSRTITQKFPATEIKNIEAKAKGTIRIYNAYSSSPQALVATTRFMAPDGKIFRLVNKV
                     VVPGAKIENGKIAPSYIDAEVVADRPGESYNIGPTRFTIPGFQGTPRYEKFYGESLKP
                     MTGGYIGQTKVVGQTDITNAKKKIADIAYVSLNEELQTKLPAGSIVLDDAKQLIVVSA
                     TTSPALGEKAEEFEMTAKVALKVLAFQESDIKHLFTAVAIKDDNSLSEKELFSYHFEY
                     GVPRVDFSDRKLLSFPVLANLVFRDPVDVNQFRDQLVGKSTKELEKLFEQYKNIENIN
                     VDIWPKFLEFLPLNSARIKISLDGQAV"
BASE COUNT        12908 a         8114 c         6602 g        14197 t
ORIGIN      
        1 gcggaagggg tgggattcga acccacgagc cctcttacga aggcacgact ttccagatcg
       61 ttccgttaaa ccactccggc acccttccaa aataaatttt taattaattt gctctttttc
      121 ttttttagac tgatattttt gaccaatcaa tttatcacgc atcatagcat agaaattgga
      181 accaccatct tttaaaatta cctcagaaat ttctttaaca ttttttccct ctttttccat
      241 cttcttaatt aattcatcaa catatttctc ttgtgctatt tttagctcct cttctgttaa
      301 agttcctttc tgaaatttat caaacaactc caattcttca tcaatcattt cgtctaaatg
      361 aataaaatcg gaatgcgggt cgctttgctg attttcaagc caacgagcaa aatttcttaa
      421 ttgctcaacc tgctctgcta aatcctctct ttgagccgct tctgcgtctg atttttgttt
      481 ttctactggt attcttggct caatttctgg cataagtttt tatttaaacc gacatttctc
      541 gtaaagcttt taccctatct tcgataggtg ggtgagtcat aaataatttt actaaccatg
      601 attgactttg cttgccttta aaaggattag caatgtataa atgggcagtg gcattggaag
      661 tataacgcat cggggttgga tcagtcgcaa ttttttctaa tgcccgagct aatccttcag
      721 gataacgagt taaaagtgca cttgaggcat cagctaaaaa ttctcttttc cgcgaaatag
      781 ctagttggat taattgagca atcaaaggcg aaagaataat aaaaaccact ccaataagca
      841 ttataattgc cgacaataaa ccacctcctc tatcatcgtt ttttcttccg ccaccccaaa
      901 aactcactct tacaaaaaca tcagccaaaa tagaaattaa tcccaccaaa acaaccacca
      961 cagtttgaat aagaatatcc cgattgccaa tatgcgataa ttcatggcca attacaccct
     1021 ccaattccga acgatctaat ttttgaagca atccttcagt aatcgcaata gcagcatttt
     1081 tgggatttcg accagtagca aaagcgttag gtgccatttc gggcaaaata tagattttcg
     1141 gcatcggcag accagcggta atacaaagat tttcaacaat acggtataac tctggatttt
     1201 cttcaaaagt cacaggttta gcatgagtca tgcccaaaac gatcttatca gaataaaaat
     1261 agctaatgaa attcattatg aggctaaaag ccaaagcata ataaaaaatt gaaggtgaat
     1321 tgaaataata acttagaaac caaccaatgg ctataataat aatcaaaaac aaacccatca
     1381 ataaccaagt tttgcgaata ttttggcttt tgaaagtata taaattggcc ataatgaact
     1441 agaattgcac tttgatcggt tctttctccg tttcttctgc ttcaaaaaat tctgctggtt
     1501 gaaatttaaa cattttagct atgatatttg aaggaaaaga ttcgattttg gtatttagat
     1561 ctaaaacaac gctattataa aaacgtcgtg ctgcctgaat tttattttcg gtatcagcca
     1621 gttctctttg aagatcaaga aaattggcat tcgctttaag ttcaggatag ttttcagcta
     1681 ccgcaaaaag tgtttttaac gtattagaaa gggcctcttc tgctttggct ttttcttgaa
     1741 gtccttcggc ggcaatagca cgagcgcgag cagccgtaac attttcaaaa acttgtttct
     1801 cgtgggctgc gtatccttta accgtttcaa taacattagg gattaaatcg taacgacgtt
     1861 taagttggac ttctatatcc gacgccgctt ctttaactcg atttcttaaa cgaataaaag
     1921 cgttgtaagt gaaaatcgga taaagaataa taatagcaac taaaataatg attatccacg
     1981 taagcatatt aatttgaaat taagcgacta taactgaatt ataacgcaaa aatataaatt
     2041 tagcaaataa aagttaaact tcaagctcta gataatcaaa tggttctcca aaatcatgcc
     2101 aattttctat cgttcttaat ttctttgctc ctcgacgctg cataatttca gaaagtccat
     2161 tggtaacccg cacgtgagct gatatttttt tatatccctt ttgttttgct tcttccttta
     2221 aagcatgaag catttcaata aaaccttcta tgtttctagc tgctggatca gtcgcaattg
     2281 attcaacata caaaacatct ttttctggtt ttaatccatt atcataatca gataactcct
     2341 tataggcggc aactaacggt ttagaagaaa gatatgaaac aatattgccc tttttatcac
     2401 gcgcaattaa ttgaactccg tctttgtttt gaatggtttc ttttagatct tcgagatctg
     2461 attgcatttc ttctggaaaa ctagctgttt ctatttttaa aatatcttct agaatgcttt
     2521 gattaatctc ttttatcatt tcaatacgat attgtttttt taccaacttc ttcttttgtc
     2581 tttcttttaa tatcccacgc cttcttatga ccaagtgttt ctagataata ttcatcatca
     2641 ttaaaatagc cacctaattt ttgaataggc ggaagagggg ttgtttttaa atcttctgtt
     2701 tcaacatcat tagtaccaac ttcacctaaa actactttgg agatatgaca ggattcagca
     2761 taacgattga ggaattgaaa cattgattcg cgaatggccc gattaacatt gtgaccagca
     2821 ggataatgat tattaacctc aaaattatca gcaacgagga cgggtcgttc attctcatct
     2881 aaagtgacga aaatccaagt ttgagcaaca acatcaccag tatctggatc aacaatctca
     2941 gctacattaa tcccttgatc aatgagatat tggaaaatgg tttcgggatg aagcgtggga
     3001 taggcgttag ctccgggtgg agcttcttta acacctacag caatacagca atgagtataa
     3061 ttaccttgaa agagatcttt atgaggatct cgatcccaaa gttttacaaa gaattccttt
     3121 ttattggtct gctctttttg ttgttgggtc gttaggactc taatggtttc aattaaatga
     3181 ttaaaagatt ctagtgcttt ggggtgaagg gtatagttgg gatttttatt ttgaagataa
     3241 tgaaggcttt tggcataggt tggtaaaagt tgttcgatcc aatggggatc atcaagatta
     3301 attttagagg gatcaaattc ttttttcttt tgattgagtt gagcgaggtc tttagctaaa
     3361 gaggatttga gttggggttc atattcatta atatcagatt gccacgcttt aaagcggttt
     3421 tcaaattgac gaaaaagagc ttctcgatcc attttattga ctgtccccac ttccatcatt
     3481 accatttctt taaaatggag ccaatcttcc caggaaatgc cattttgctg aaagagttgg
     3541 gctacttttt gattgtgctg ggctattgct tgaccaattt catcttcttg attgagattg
     3601 gtgataaaat catcaaaccg gttctcaaaa acggatttga gaaaatgatc gcacatttct
     3661 tgaatgtccg gagattcttt tttaagaaat tcttgattag taataaggtt agcaatatat
     3721 ttaatcttcc actgagtaag atcttgatct gtaatttcaa cttgaatctg aatactttcc
     3781 gctaatttct ttaagagtgt tttgttaagg agtgtcttta attccgagaa gtctttgcca
     3841 agatttttct gaagaagatc ttgaaaaagt tgaggattat ctaaagagag ataagcagcg
     3901 gcaatctcta aaagatcgga aaaattgtag ccatcaagtt tttgattgga ttggagatat
     3961 tctttaatgg attgtgtcag tgctggatga attaaaacag gttcaaaaac ctgaagatag
     4021 ctttcagtga aggataaacc acgcaacaga tgattcttta aaagttgagt agaaagattg
     4081 agatcaatta attgaagacg ggcaatatcg taattgtatt gtttaaagaa atcttcgggg
     4141 ttttgccatt ttttggattc tcttatttcg ctattgatct taaaggcata agatttaata
     4201 aaacgatatt gaggaagata aactttaatg aattgagctg gatcttcttt gtgttcaagg
     4261 agattagcaa gtaaaacata acaatagttt aaatcatctt cttttagttg ataatcatta
     4321 attaaaacag catctatttt tttaaaaaag tcataattga aatgagaggc tagttctcct
     4381 aaggatccag ccactgccaa tctaacattt gaattttcat catttaaccc tcgttcataa
     4441 aaagcaatag ctttatctgg agcgacctga gctagttctc ttaatgattc gaccgtggct
     4501 tctctgacag tccaatcttt atcattaaat cctttttcat aaaaagaaat agctttgtct
     4561 ggagcaactt gagctaattc ccataaggat tcggctgcct ctattctaac agctggattt
     4621 ttatcattaa atcctttttc ataaaaagga agagctttgt ctagagctgc ttgagctaat
     4681 gtccctaagg attgggctgc ctttattctg acaaatggat cttcatcatt aaagcatcgt
     4741 tcataaaaag aagtagcttt atctggagcg acttgagcta gttctcttaa tgattcggct
     4801 gcgcctattc taacgtctaa gtctgtatca ttaaagcatc gctcataaaa aggagtagct
     4861 ttatctggag cgacttgagc taaggcgcct aaagattcgg ctgtggcttt tctaacattc
     4921 tcgtcttcac tttgaaaacc tcgttcataa aaagcaaggg ctttgtctgg agcgacttga
     4981 gctaattttc ctaatgattt agctgccatt actctaacag ctggattttt atcattaaat
     5041 cctttttcat aaaaagaaat gactttgtct ggaacgattt gagctagttc tcccaaggat
     5101 cggactgtca ctactttaac atcagaatct ttatcattaa agcattgctc atagaaagca
     5161 agggcttttt ctggagccac ttgagctagc gatcctaaag atccgactgc ctctactcta
     5221 acgtctggat ctttattatt gaagcatcgt tcataaaaag gaatggcttt gtctggagcg
     5281 atttgagcta attttcctaa ggattcggct gccactaatc tgacatactg attttcatca
     5341 ttaaatcctt tttcataaaa aagaagggct ttgtctggag cgacttgagc taattttcct
     5401 aaggatcgag ctgcacttgc cctgacatat gaatctttat tattaaatcc tttttcataa
     5461 aaaggaaggg ctttgtctgg agctacttga agtaattctt ctaaggatcg acctgcccct
     5521 actctgacat atgaattatt atcattaaag catcgctcat aaaaaggagt agctttatct
     5581 ggagcgactt gagctaaggc gcctaaagat tcggctgtgg cttttctaac attctcgtct
     5641 tcactttgaa aacctcgttc ataaaaagca agggctttgt ctggaacgac ttgagctaag
     5701 gcgcctaagg tttgggctgt ggcttctcta acatgatcat ctttactttg aaaacctcgt
     5761 ttatagaatg gaagagcttt gtctggagct acttgagcta aggcgcctaa ggtttgggct
     5821 gtggcttctc taacatgatc atctttactt tgaaaacctc gtttatagaa tggaagagct
     5881 ttgtctggag ctacttgagc taaggcgcct aaggattcgg ctgtggcttt tctaacaagc
     5941 caatcttcat cttgaaatcc ttgttcataa aaagcaaagg ctttgtctgg aacgacttga
     6001 gctaaggcgc ctaaggatcg aactgtgata tatctaacat cgccatattt gctttgaaaa
     6061 ccttgttcat aaagcattac cgtttttaaa ccattaataa tctcttgttt ctttttttct
     6121 ttcagctctt ttaattcttt aagttttaat tcatctttat tagcaattga tttaatggct
     6181 tcataaaaaa gctgatcttc ttcatctttt gaaattcttc tttttaagag ttcaccttcg
     6241 aactgccatg gttctttgcc tggttgaagt tgagcaaatt ttaactgcaa atcaactgga
     6301 agaatttttc ttaattcttc ccttttttct tctctttctt tgatttgctc tggtgtacga
     6361 ttaatttttg gcctttcttg agacatatat atattataac atatttagat gatggaatca
     6421 agattcacgc cattccttaa ttttttgatg atggccggaa agaagaattt taggaacgcg
     6481 ccaagcaatt tttttatttt tgggattagg ataaaaaacc tctgggcgtg tatacacagg
     6541 ataactacct tttatttctt caagtgattc gtatttgcct aaaacgccgg ggattaaccg
     6601 tgaaactgca tctaccacaa tcattgcggg aatttctcca ccgcttaaaa tataatcacc
     6661 aacagaaatt tcttcatcag caatatattt tgctattctt tcatcaatgc cttcgtagtg
     6721 gccacagatt aaaattaaat tttcatagtg ccttaagcgt ttcgccattt cctgattaaa
     6781 cttttcccct ttcaaagaaa aaagaataat acgagttttt tgtttttttg atttgatata
     6841 tctcttttta ataaattgca ctgctttata aattggatca attttcaaaa ccattcccgg
     6901 ccctccacca tatggtctat catcaacttt tttatgttta tcaatgctaa aatcgcgtgg
     6961 attaataatt tttatattaa taatcccctt cttctgagca cgttttaaaa tagagatgtt
     7021 aaaataacta aaagcctcag gaaaaagtga aataatatta aaattgataa ttttttgctt
     7081 atttagtttt ttgcttgata ccttcatctt gtttattgta atatcttttt tttagaaaat
     7141 gaaccccttt taaggggttc attttcttat tgatactaat taataaagat tgggagacta
     7201 gttaaaaatt ttctagactt tcgacttctt tatcaaccga actatctgtt tttctcgaag
     7261 aaccttctgg ctcttcaatt tttaagttca ctcgagcgtt gtggcgaaga ccgataagtc
     7321 tagttaaaga tcgaatggct cttacggttg agccttgtcg accaataatt cggcccatat
     7381 cctttggatt tactttgaga gtcaaaagca ctcccatttc atcaattgtc cgcgtaactt
     7441 taacatcctc aggataatca acaagtgctt taatgatata ctcgagaaac tcttgatctt
     7501 ttggaatttg attattcatg atactcagaa cagcgtttct ttaagtcgac ctttgctgga
     7561 tattttatat tataaagaat gaaaaaagta agtcaagaat gatatatact acaatttcat
     7621 ttaatattcc tgggacggag ggttttgaaa aaacgctgta tttcttaatg atcttgctaa
     7681 ttaattttag ctaaagtaaa atatataaat ggctttaaaa aatttagctt atgatattaa
     7741 ttattggctt aagaaacaag ggaactgaat ttcaaaacac ccgtcataat attggcgctg
     7801 aaataataat gcgctttggg gaaaataacc acttccaaaa atttaaattg gaaaagcgac
     7861 tgcaatctct catctctaaa aacgaggaaa taattttggc tatcccccaa tgctttatga
     7921 atgaatcggg aataatagta aaaaaattgg tagcttattt tgaaattcca ttagaaaata
     7981 tattggttgt tcatgatgat accgatattc gattaggcaa atttaaaatt caaaaaaata
     8041 gaggggcggc tggtcataag ggtttggaat cgattattgc taatttaaaa tcaaaaggct
     8101 tctggcgttt acgaatcggt atccgctcag aaaaattaat ttcccaaaag gctgaagata
     8161 ttgttctaaa aaaattcagt caagaagaac aacaaatact taaaaaaatt gaaccagaaa
     8221 tttttgaaac cattaatcaa tggatcaaag aagtcgccct gtaagagtgg atatcttgtt
     8281 aattctcttt ttgattaaga tcgtttaaaa ttgaactatg actccatcag ccaacaaaga
     8341 atatatcaat aaaaaattac ctcctcaaaa cattgaagcc gagcaatcta ttttaggttg
     8401 tttaatgatc gataaagaag ccattacaaa agtagctgat tttttaactc ctgatgattt
     8461 ttatcgttcg gctcatcaaa aaatatacgc tgctatttta gatctttata gcaaacacga
     8521 gccaattgat attgtcagcg ttactaatcg attaaaagaa aaaaaacaac taaacgaggt
     8581 gggcggagca agttatttgg cttctttaat taatatggta ccaacaagca gccacgtagt
     8641 aatgtatgct aaaatcgtcc agcaaaaacg aattctgcga gatttaatta gtgccgcata
     8701 tgaaattggc gaaatggctt ggggagaaga caaagacgtt gatattctcc ttgatgaatc
     8761 agaacaaaaa ctcttccaaa ttactcaaaa atcattacgt caggaatttt tgccgttaaa
     8821 acccatgctg acaaaggctt tcgaacgaat tgaaatgctt caacaaggag gaaagcagtt
     8881 ttccggcatt cccactggtt ttgccgaatt agacaaaaaa ctatttggac ttcaaccatc
     8941 tgatttaatt attttagcag cgcggccttc tttaggaaaa acgtcattag ctctagatat
     9001 tgctcgccat gctgcagtta aagaaaagaa aagcgttggt atttttagct tggaaatgtc
     9061 taaagagcaa gttgttgatc gcttgattag tgctgaatca ggcattgaac tttggcgcat
     9121 tcggacagga aaactaaatc ctgaacattt cgaaattttg caacagggtc ttgatcgact
     9181 ttctcaagcg cctatttata ttgatgataa ttcttcgcct aatattatgc aaattcgaac
     9241 gatggctaga cgccttcagg cagagaaagg actagattta attattattg actatttgca
     9301 acttattgtg ccgcgggaaa attataatgg cagtatggtg caacaaatta cggaaatttc
     9361 cagaggatta aaagcgctcg cgcgagaatt acaaatcccc attctagccg tttcgcaact
     9421 ttctcgcgcc gtcgaacaac gagatcataa aattcccaaa ttatctgacc tgcgtgaatc
     9481 tggatctatt gaacaagacg ccgatgttgt tctctttata tatcgcaaag atcgcgatca
     9541 gcccgaatct gatcttaccg aagaagaaaa aaacacagcc gaaattatta ttgctaaaca
     9601 ccgcaatgga ccactgggaa caataaaaat taaatttatc cctgaattaa ctacttttcg
     9661 taatattgat gccacttttg gtgaagaaaa taatgaaggg gcggattttg gttggtctat
     9721 tctagaataa tattatgtat cctgattcaa ttaaaaattt agcagatgtt cttgcgcgtc
     9781 ttccttcgat gggaaaaaga caatctttaa gattggtttt ttatttgatt cgtaaccaag
     9841 aaatacgaag aagacttcta attcaattaa atgagctaga ggaaaaaata aaaatatgcg
     9901 agcaatgcgc tcttcctttt gaaggagcgg gacaattatg taatatctgc aataattcct
     9961 cacgcgcaca aaacatcatt gctattgttg aaaaagaaat tgatgttttt tctatagaac
    10021 aaaccaactc atttaaaggg acttatcata ttcttggcca tttaattgat ccctcaaata
    10081 ttaatagtta taataccttg cggctggatc aactagaaaa acgaatcaga agactgcccc
    10141 aacaaattgc tgatgaaatt attattgcca ttagccccac caccgaaggc gatttaacag
    10201 ctatgtattt ggaacgcaaa ttaaaaaatt tagctaaaaa aattacccgc attgggcgcg
    10261 gaattccaac tggcggcgaa attgaatttg ccgatgaaga aacattaaaa aatgcactct
    10321 ctaatcgtca ttaaacatgg aagataaaat aaaaaactcc ctttaacgga gttttttatt
    10381 ttatcttggt aattttaagc tctgtcaacc attgacgatg gccctttttt ttgcgataac
    10441 gcgttttgct gtgataacga aaaacggtta ttttttttga tcgtccggtt tttaaaacct
    10501 ctgcttctac agaatgcgcc tttaagtgtg gcttgcctat ttctacttta ttgttatcgg
    10561 caattaaaag cacgcggtca aaattaactc gctcacctgg ttttccttca attttttctg
    10621 ttttgatttt cttcccttct tctaccaaat attgttttcc tcctgtttca ataatggcaa
    10681 acatatggtt atatcttaga aattaatttt agtttgtcaa ttgacttttg ttcgcttctt
    10741 tattgtcatc cgtagattga tgattattct cggcttctcg ttgagaaata aatttttcta
    10801 ttttttgagt aatcttttca aactgattaa taatgtctaa aatttcttca gctgcttctt
    10861 cccacaaaaa ttcccctatt tccttggaag cttttgatgg ctcaccataa acaccgcttt
    10921 ttgattcata ttcccatggc aaaaataatt ttaacgtgcc ctcatccaat ggtttataat
    10981 ctttggtttt taataaaccg acttcatcga caattttatc tttgcttact aatttttcat
    11041 tcatcgccat cattaaagaa gtttctattt catcggcgtg acgaatccag tatttataat
    11101 ttaccttttt tagagcctga atacctcgtt tacctgacaa atacaaggga atggttgccg
    11161 cataagggta ttctacattc aaagtggcaa taacatcttc aagatagctt tgattgccgc
    11221 cgtggccgtt caaaaagaac attttacgga aaccgttatc taaaacgctt tggctaatgc
    11281 ctaaaacaat ttgcttaaaa gttttatcgt caacattaag agatcctgca tatccccctg
    11341 aaactccata tgacaatgtg ggaagaagga ctattttatc tttagccttt ccttcaacac
    11401 gcttagcaat attttccgcg ataaaagcgt ctgtgcccac tggcaaatga aaaccatgtt
    11461 gttcaattgc tcccagcgga aacaatgcta caacatgacc cttgtttaaa tttttaattt
    11521 cttcaacggt taattctgaa taaatcatat ttgtataatt ttctcataaa aaaataaaaa
    11581 attcaatctt tttcgcgcac ataaggttta ttataaaaat aaatgcttaa ataatttaaa
    11641 aagtagcgca gggatgtttt aatacttccc tctttctgga aacgacgatt agaagacaaa
    11701 actttaagat gagatgaaaa aactactctg ccctctttgg ctaatcgcag cgccgtcatt
    11761 acatcttccc cccaaaattt taccgaagta tcaaatccac caattttttc taaaatagat
    11821 tttttgataa aacagtttcc gccgataata atagcggctg gtttacgaaa aataaaatgc
    11881 aaaaatggag ggataattgg ataaaaaatt gattggaata acaaagaagt tcgtcgaaag
    11941 aatacagggg catcaaaata atcatatgga cccgatgcag caataacatc ggatttttgc
    12001 aagtattttt ctgccttttt aagccaatcc gaaggaacgc gacaatcagc atcaagacaa
    12061 acaataattt cgcccgaagc agctaaaaat cctttttgcc gcgcccatgt taatccctgt
    12121 cgctcttctt taacaatttt attaactccc catttttgag ccaccgcaac tgtttgatca
    12181 gtacaattat tatcaactat gattacttca aattctcggg aaggataatc cagttgtctt
    12241 aaggattcca ataaaaatgg aagtgttttt tcttcattat aacaaggaat aactattgaa
    12301 aatttcataa tttttagagc gggtgaccgg atttggaccg gcgatcttct ccttggaagg
    12361 gagacgtgtt gccactacac cacacccgca agatattatt attttaacaa ttctttattt
    12421 tttgacaata ttttttataa aagtttttcg atgaatttta ctaataccat tttttttaat
    12481 taatgaaaag tgcttttttg tcccataacc tttatgatcg gcaaaacaat aatttatttt
    12541 tttatccatt tttatcatat ataaatctct ttttacctta gcataaatgg aggcggcaga
    12601 aataaaagga attttatcat cgccttgaat gattgctttt tgattatatt tttctggcgc
    12661 ttttaatccc ccatccaaca atactaaatc tattttttta tcaatttttt ttaataatcg
    12721 tctcactcct tcttttaaag ctttacccat tccatattta tcaataaaat tattactgat
    12781 aaatacacaa taaaaattta aatcagaatc tttaaacttt tcatgccata aaaaacgttg
    12841 attaaatgtt agtttttttg aatctttaat tcctattaaa aattttttat ttttattatt
    12901 taaatatccg gctgcagcgc ctaaaactaa aggacccgct atcgctccac gtcctacttc
    12961 atcaatgccc acaataaaaa attgtttgga ttttttattc ttttttttca ttattattaa
    13021 gaaaactaaa aaagattttg tttaatcagg ttggtggttt ggtggcctgc tgtatttcac
    13081 cagtttcatg caattgcttt atcttgcatt ataatctatt taattatcgt taccttaaaa
    13141 ataattgtac ctcgcgtcta aatcaattat agaaacaggc gcgcgttttc aatgatatta
    13201 atttttaaaa aatcggctgt tttatcgccc gtcaacaact ttctttttta ctaattcgct
    13261 tattagtaca acatcttcag tcgggcgcat ctaataaaac cgatccgcta ataattgcgc
    13321 caataagcaa gccgttgtct gaaattaaaa caacgcctca attaaaaaag tctgctgttt
    13381 tgttgatcta aacacagatt aactagctta tcggctaaac gattttgttc tctgggaata
    13441 taacgaaatg agagtttgcc aaaatcaaat tttaaattcc aaatagtcat aaataaaggc
    13501 tgaagttttt tatcttctat tttatattcc ccatttaatt gtttggcaat taattctgaa
    13561 tctaaacgaa tttctactgg gatttgtttt agccgctccc tccctatgag ctgtctaatc
    13621 tttcttaaag caaaaattat agcttgatac tcagcctcat tattagttgt ctctcctata
    13681 aattcattat attgctttgg ttcatgagac actccttcaa caacaactcc gatagcggct
    13741 ggtccgggat tgcctcgact acctccatct gtatatgcaa taattttttc atttgcgttt
    13801 tttttcatat tatttttctt ttaatcccaa aagttgaaat tccaaaaatt gctgccctcc
    13861 ccattcttcg gttcttaaag aaaaaactat atctgcctct gatccgcttt ttaaaaattt
    13921 taactgttca gcattattaa aactaataac gttaaaataa cgattttttt tctggatgct
    13981 aaatttaata tgcttgccgc tattgcccac aattcttatg tctgaaatca caacatcaga
    14041 catcaaaaat attggttcgg gattttctcg accaaatggt tcaaaacgat aaaaattttc
    14101 taaaaactga gaagtaattt cgtctgcatc caacgatgtg tcaataatta atttgggaat
    14161 tagatctaca tctttaatgt tactcccttc ttcttgtagt atttctttta aacgtttaag
    14221 gttttcgttt tttaaagaaa aaccagcagc aagacgatgc ccaccggctt caataaaaag
    14281 atcttgatga cgccctatca gttccactag attaaaacca ttaatagaac gagctgaacc
    14341 agtgctggtc tctttccctt ctgaataaat aaaaacgggg cgattaaatt catcttttaa
    14401 tttccctgcc acaataccaa taactccttt cgaccagttg ggattgccca aacaaataat
    14461 cttatccaat tttggttgct gagacaattc ttttttaata gaatcataaa tttcttgtac
    14521 cttattttgt ctttcaagat taagatcgtt tacttttcga gctaaaaatt cggcttcatt
    14581 ttcgttttcg gtcatcaata gctcaaaact ataatcggtt tcttcaatat tgtcttcaat
    14641 gttatgttct actcgaccaa cagcatttaa acgcggggca ataataaaac caacgtgata
    14701 gggatccagt tttgtctttg gattaaggcc gactatctct aaaagctttc ttaatcctat
    14761 attttgagtt ttgactagtt gcttcaatcc ttcgctaaca aaaatgcggt tttcgtcaat
    14821 tagtggcgcc acatcagcaa ttgttgcaat cgctactaaa tcaaggaatt gtttttccaa
    14881 aataggtttt aaaggatggc gattggtttc caataaaaat attgctaatt tataactaat
    14941 gccactagct actaaatcct taaaaggata ggggtcgttt ttttgccatg ggtcaacaat
    15001 aagtgtatct ggtaacttat ctggtaccaa atgatgatca aaaataataa cctccatgcc
    15061 taatcgtttg gccaaagcta tttcttctat gtcggtaata ccacaatcta aagtaattat
    15121 taggttcgtt ttacgttgat gcatcttttt tattttttct tctgttaatc cgtagccccc
    15181 cctaaaacga ttgggaatat aaacatcgaa gttgtgataa tttagtattt taaaagtctt
    15241 ggctaaaata cttgttgaac acactccatc ggtatcataa tcaccgtaaa ttgcaattct
    15301 ctcttgattt tttattgctt cattaattcg ctgcaccgcc ctctccatat taagcatttc
    15361 ttttggatga taaagatggt gaagagcagg attaagaaat ttataaatac tttcttttgt
    15421 tcttaaattt ctattccaaa ataattgcaa aaggggtcga ttaaattcgg gaaatgactt
    15481 ggcaaattcc tgtggcattg attctctaat tttccattga atttctttca tattatttct
    15541 ttaaaaaatt aagggcgggc aactcgcctt ttgctaaaaa ttcaagcatg gcgccaccac
    15601 cagttgaaac aaagctataa cgatttaata aatgcaattc ttcaataact gttaaggttt
    15661 ctccgccgcc aactactgta aatccttgat tattcgcaat agcttgtgca attttgattg
    15721 tactatttcg aaaggctttg ttttcccaat atcccatagg gccattccac aaaatagtct
    15781 tagcggattt aatcagaccg caaaaagcct ttgtggctat agggccaaca tcaacgataa
    15841 tatctttcgg ccctatatct cctatttctt ttacttttcc cttcgttgat ttaaaagaag
    15901 aagcaaccaa aaaatcagcc ggcaccacca gctctgcttt tctagaccct aaatttttag
    15961 catcgctaat tttttctgct tcaaataaag atttacctat ctcaaaaccc catgccttcc
    16021 aaatggtatt agctaaacca ccacccaaaa gaatcgtatc agctttttcc aaaaaaacat
    16081 ctatcaaagg taatttcgta gaaatctttg caccacccaa aaccacaaca aaaggcgaga
    16141 tagcgttttt aatcaatcga tttaaattat ttacctcgtt agtcaaaagc aagccggcat
    16201 agcttggcaa aaattgagta atcgctgcca cggaagcgtt taacctatga gagacgccga
    16261 aagcatcatt gatatacaca tctgccaacg cagctaattg ttgggcaaat tttttatcat
    16321 tattctcctc ttcttcataa aatcgaaggt tttctaaaac aaaaattctt tctttgcgaa
    16381 tattaatttc attttttatt tgagcaccga tgcaatcttg aagaaaaact actctttgtt
    16441 taagaagctg ctctaagtgt ttagcaatgg gtaataaact taatttttta tcttttcctt
    16501 tgggacgacc taagtgacta ataataatga tcttttctgc ttggtgcttt aatagaaatt
    16561 caattgttgg taaattagct cgaattcgaa aatcgctaag gatttttcct ttgtcatcta
    16621 aatcaacatt aaaatcaaca cgtaataaaa tttttttgtg actaatggct ttgggtgtaa
    16681 tcgtttttat cattttatct catagatcaa tgacactgat acaacaattt cattagttcc
    16741 tatttctatt ggagcagttg catctgctga ttcaatcgtt ttttggtaaa ctattcgctg
    16801 tggcgttaaa ccgctaacgc tttcgttgat ctcgataatt ttacctaatt caatattagc
    16861 agcattagcc atttcttggg ctttttgttt tgcctcagca atagcttttt gtcttgccgc
    16921 agctttaatt tctgtatcat tatcaataac aaaactaata tcggaaattt gattaacgcc
    16981 aatttcgctc aaatttccaa taatctttcc aacacgactt aaatctctaa ttttaacctg
    17041 aagagattgt gtaagcatgt aatcaactaa ctttggtgga caatagacgg gatattccgt
    17101 agtacgacac cagctataat cgtattcggg ttgaagatta tattgcgtag ttttaatatc
    17161 cttttcatca atgcccgatt cttttaaaaa attgaccact tgctgcattt tttgatcgtt
    17221 ttcactttgc actaattcaa catttttatg ttgactaata acagacattt ctacgattgc
    17281 cacgtcgggt ttagcaaaaa tttttccttc tccgctaacg gttaaggtat gctccggggg
    17341 catattgctt ataacatgag aaacgcccca acccgcaatc aaggccacaa tagaaataat
    17401 gatcaaaaaa atccctaatg tatattgcca aaaactggat ggatttattt tagccataaa
    17461 ttttattaat aagaattggc gattgttata acattcacca attcctcttt ttttatactg
    17521 gcgcctccga ctaaaacgcc attaatttcg acttgtttta aaaaatcatt tatatttttt
    17581 gaatcaacag aaccgccata aattaaagtt aaattcggca ttattgtctg cgctgcgctt
    17641 tcaacagtcg attgagaatt tcgattatct gatgattccg attggtaagc gaaaatcgca
    17701 ttaatagtgt tttgaataaa tttcattgct tggcttactt caataggatc acaaaaatct
    17761 cctgtcccaa tcgcccaaac tggctcatag gcaataaata agctgtccaa ctgcttgaaa
    17821 taatttgtat tggaactgag tgcttgttcg agttgatttt ttaaaacaac ttccgtttcc
    17881 cccgcttgcc tttcttgctg agtttcacct atacaaagaa tgggcgttaa attattttgg
    17941 ataacagctc gaactttttt attaattatt tcgtttgttt cacctaattg tcttctttcc
    18001 gaatggccta aaataacata ttgcacaccg aaatttttta acattaacgg tgaaatttca
    18061 ccggtgaaag cgccctgatc ttcgtaaaag caattttggg cagccaactg caaattcaaa
    18121 ttcttagccg tttctaaata aagaaagggc ggagcgataa ttaaattaac gttctgattt
    18181 tttttacttt cttctttata tagggttagc aattgtttcg cttcttcact agtggctgga
    18241 ttcattttaa aattagcaac aattgttttc attaaattta ttatagcata attatcgtaa
    18301 aattcaaaaa atacacataa tcactccttg cttctttaaa ccattttata cttatatttt
    18361 tagggatata atcactctaa aaaataagga aataaatata aaaaaatacg gccttgcgac
    18421 aaaaaatggc tcatccatct aaaatattaa ctttcgttat cgtaaataat tgttgggtgc
    18481 tttagataaa ttcttttaat tcggtattgc gaatctcgtt ataaatatcc agtaatgccc
    18541 ttacaccatc gccaatagct acgctaattt gtttgtaagg caaattagta atatctcccg
    18601 ctgcccaaac tttccgatga gaagtccttc cggaaagtgg ctccacaatt atttcttctg
    18661 cctcattcat ttttaataaa ttcttaaata gttcgctatt cggttgcatc cctgcttgaa
    18721 tgaaaatgcc actcaccgca agactatgtt cccgtaaatc attcccctct ttgtacttta
    18781 aaccgcttac cattttatct ccctcaatag atttaatttc tacattataa tgaatctcga
    18841 ttttgggatc gtgtttaatt cgatcgatta aaacggggtc acagcgtaaa tctgattcgt
    18901 ttgttaataa ataaatttgc gaagcaaaag gtattaaatc aataacgcta agaagagctg
    18961 agttgccgcc gcctattaca gctactcttt tgttcctcat caacggcgca tcgcatgtag
    19021 aacagtaaaa aacgccttta ccttctaatt ctttttcttt cggcaccccc aactgacggt
    19081 aatgacgtcc taatgttgct aaaattctct caccataaaa agtcccatga tcggttttaa
    19141 tttcaaaaat tccctcctta aaaacaactc catttactcg atcaataata atttccaatt
    19201 cggtataatg acgaagttgc ttttcaaaac gccttgccat ctcaatccca ctaatttctt
    19261 caaaaccaac aatgttttca attttggccg ctactactgc ttgacctcca aaattttcag
    19321 taataattaa ggtgtcaatt tttttgcggg ccgcataaac acccgctgcc gttccggctg
    19381 gtcccgcgcc taaaataatt aattgatgct tttttacttc atccataaaa attaattttt
    19441 ttcttataac tcttcggaaa tttcttcaga aataaaacgc tctttaatct cttgctcaat
    19501 taaagcccgt ggtcggccat atgtgacgcg cgatagctct ttaagaactt tggctttacc
    19561 agtatcgcct ttaagagggg gtatggttct catagtaaaa gggcgtgttg ttttgttatt
    19621 aaccatcaat ttaacaatag cagtaaaatt atccaaatta atcatatcat tttcattaaa
    19681 aacagggctg aattctttaa ctaaaaattc ggcatccttt acgcccactc gaaacactac
    19741 catacttcct acattaccaa aaactgcttc tcgaatttta tcttgcaatt gagcaataaa
    19801 ttgatgactg atagtcagat ctaatcggta tttccgcgct tcagataaaa tagtagcgat
    19861 acttggcgtt gtaaagtttt ggaactcgtc tatataaaga tagaaatctt tgcgctcttc
    19921 ctcaggggta tccacgcgcg aaaatgccgc cattaataat tttcctacaa taattaaccc
    19981 caaaagagaa gcattgagtt ccccgatttt tcctttggat aaattaacta ataaaatttt
    20041 ttggccatcc ataatctctc taaaattcaa actggaatgt ctttgggcaa taataggccg
    20101 catgtagtca ttggtgataa acgtattaaa cttacttgta atataaggac taatattctg
    20161 caatgataaa tcactactcc ctgaaacttt tagagcctct ttttcccaaa attctacaat
    20221 cattggatca ttactattgg ctaatttttt ctttaaaaaa tccggattag taaaaatacg
    20281 tggaacgtca attaaagtag gcgtttcagt agtgtcgctc attaataatc caacagcatt
    20341 tctcaaataa tactcaaaca tcggacctcc cacctgactg agatcgtata atttatcaaa
    20401 aatatctatt aaatcgttaa taataaaagt tttttgttca ggtttgctta tatcgtattc
    20461 gagcatattt aatcccattg gcatttctaa gtctgatgga tcaaaaacaa caacgtcttt
    20521 aaatcgttct tgggggacta atcccagaag tgtttcaatt acatcaccat gaggatcaac
    20581 aaaacaaaca cctctacctt cttcgatatc ctgagcggcc atattaatca aaaaaacaga
    20641 tttaccaacg ccggtttgac ctacggtata aacatgtctt cgacgatcat ttggtaaaat
    20701 tcggactgtt ttcttctgat tttgataaac agcttctcca agaacaatac cctcgtcagg
    20761 caattctatt ggcggcggcg cttctcgagc ctttacccat tttaattgag gagctacttc
    20821 caccgttaag gggaaatgcc aaatagaagc aatttcttct gtattcaaat acatcgcttc
    20881 tttattatta aaaagccgaa aagagtaatt aaaaacaaac ttattaaaat tggaaggctt
    20941 aactgcttta aagttatttt ttaaaggcgc tgcaaattga tcaaaagtcg acaataatat
    21001 atttagcgcc tcatcagttc tttccttagt ctcagcggaa actaaaatgc gaacatttac
    21061 cgcgcaaaga ggcttgctga ccttcgcttc taataattta atagcgtttt catcgagcac
    21121 ttttggcttt ggttcttctt tgcttttatc tttaaccgaa acagaagaaa cgaattcttt
    21181 ggcaaaatca ccaaacgaat attttccgag tacatcttta aaggattgcc cttcttttaa
    21241 atgttttatc gctttggtta cttgagtttt aaaacttttg ggggtggggg aaattattat
    21301 ttggacgacc atcccttctc cttcttcctt taatttaaaa gcatttaaaa tagggttaat
    21361 tggatcagct tctaattcgg tataagtttt aatagggagc atccaatttt ctccttgttt
    21421 taaataaaca ccagtgctaa aaccattggg attaaaaata ttataatctc gtactttttc
    21481 cacataggct ttaggataaa aacttaataa atgcttttta aatgattctt ctagatcttg
    21541 agccactgcc gcataataag caatctcttt tcctatttcc ggcaaagcaa tttcaaaaac
    21601 aatcggcttt ttaaaattag ccaggatgct tattagctgc tctgcgatat taattttttc
    21661 ttttactgtt tgattttttt cgctaggttg ttcctcgggg ggaattttaa ttaaaaatag
    21721 attatattta aaagatttct ttaagaggct tgaaaaaata tttcttcttc gcatctccaa
    21781 tataagaaac atcacaaggc ctatagctac aataagcgca ataatgttta agggtgtaag
    21841 aagagagtta atcattgaat taaatctttt tttgctgaat taaaatgttt aaaaattcat
    21901 cgactaaagt atcatgcaat ccatcaacat aatagggtcc taatttttga gcaattttaa
    21961 ctgcatccgc tattgaatct tgtaatgcca ctcttactat tgctttaatt ctttcctcct
    22021 cgggtatttt tttaatttcg gataatttca aagagacccc atcgtcagtg ataggcgtag
    22081 ctgtttgagt agtgggcata gcttgattaa ttttcttttc cacaacttct cgcactactt
    22141 ctcttttctc aagcacttcc ttttgttcac ctaaccgctc ttgaaccttc tgagaaattt
    22201 cttctaattg ctggggatta aaattgggtt gttctgtttt gaactcagcc atattcgtga
    22261 tataatttct ctaatgagca taaaattaac gacttgcttt cttgattatt tttattataa
    22321 caagtttttc ctaaatttcc aacatcttct acgttttatc aaggctatgt cctaacgcaa
    22381 cgatgaatag aaatcatata ttcacaatct ctaaaacagg ttattttaaa attgtttatc
    22441 ctaaaaataa aataaaagaa ctatttctcc atatcttttg gtaaaaatct cttggtaaaa
    22501 atctttatga atattagaat attttagtcg caataaagta taaattctat gtttcctaat
    22561 attatttcta ttgctcaaaa aatcgttcat aaaaaaattg cttcgcattt cattattttc
    22621 gttatcatta tcgccggctt attaatgctt cactcctctt ttcaagaatc ggcgactatg
    22681 gatgaattag ctcatattcc cgctgggtac tcttatttaa agtttagaga caatcgtctt
    22741 aaccccgaac accctccttt gcttaaagat ctttcggcca ttcctctttt gtttctcaat
    22801 cttaaatttc ccaccgattc tccttattgg caagaaggtg ttaatagtca gtgggacata
    22861 ggtcgcgttt ttttatatga aagcggcaac aatgccgacc aaattattca gtgggctaga
    22921 attttcccta ttcttttaac cttgctaaca accgtattgg tttatttttt agctaaaaaa
    22981 atagtgggtc cttattgggc gctactacct tttgttcttt ttgctttttc tcccttggtt
    23041 ttagctcacg gacattatgt cacaactgat ttagccgctg cttttggcgc tttaattatc
    23101 tttctttttg ttatacctaa attgattcag ccctcgccaa aaaatattat tctggctggc
    23161 atcgctttgg gaatcgctca attaactaaa ttttcacttc ttattttcta tcccctcatt
    23221 tttctttttt tgattatttg ggaaattatc aaacttattc gtcaaaacag aaatcacgag
    23281 tcatcaccag taattaaaca actgctcaaa accattggta atctttttgc tgtttttgct
    23341 atttctataa tagtaattta tcttgtttat ctgcctcaaa atattaatta tcctcaggaa
    23401 aaacaaacaa gtgatacaga acaaattctt aattcttttg ctggtggccc agataaagaa
    23461 ttagttactt gtcgtcaatg ggttggttct ttaaaccgcc aaatgaggtg cttggctgaa
    23521 ctcgatatca aaatgactca aaataaattt acgaaaccat tagctcaata cgctctcggt
    23581 gcattaatgg tagtgaaacg atcctctggt ggcaacacgg gctatttttt aggaaacgtc
    23641 tctgcaactg gttggcattc ttattttcct attgtctttt taattaaaga acccatccct
    23701 tctcttgttc taatgttttt tgctctgttt ttagcgataa aaaatttttt cagcgctacc
    23761 acaaataata tcaaaagtaa aatttggtgg caaaaattaa ttgattggct ttcccacaat
    23821 tttctacttt ttagtatttt aatttttgtc attgtttatt ggatatcttc tatttcaagt
    23881 cctttaaata tcggctatcg ccacttattg attatcactg cgccaatgtt tattttgacc
    23941 actgatcaaa taaaaaaatg gtggtgggga atcaaaaata ccccatcctt taaattgttg
    24001 ctattcaaaa taatgacctc ttttgatcaa ttaaaaaaga tcggcgctaa aagtgctttt
    24061 atagccatta ttcttatttg gtatcttgtt gagacaatag tcattgcccc tcattttata
    24121 gcttatttta atgaattcgt tggtggtgcg aaaaatggtt ataaatatac cgttgattca
    24181 aatcttgatt ggggacagga tttaaaacgt ttagcaaaat tcgtagaaga aaatcatatt
    24241 gaaaaaatta aagttgatta ttttggtggt ggctctccgg catattatct tggcgataaa
    24301 tttgaaccat ggcaatcagc caaaggaaga cccgaaccaa attcatggct agctgtttct
    24361 ttaacttttc ttcaaaattc tcaaggcaaa cccgtgcccg gatttgaaag aaagccagag
    24421 gatagttatg aatggctaaa aaataaaacg cccgatgctc aaattggtta ttcaatattc
    24481 gtttatcact tttaattgac aaattaaaaa actatcgaat aattttcgca aatgtggata
    24541 acttaaaata atagttttta aaaagagtta taataaatta gactctgctt tataagattc
    24601 cgggtcaaat cgttatacca agggaaatca acagcatttc agatcgtggt ctggaattga
    24661 gattacccac ttatgattcc agccgggata tcttaaaagc ggagtcaact aaaaccccac
    24721 tagatccagt gggtttttgg tttaaaacat tatatcctct tccgctcttt tgtattcaca
    24781 tagttcttcg ggtgcaaacc aaaaagatat ttcatgttca gcttcttcgg ttgtttcaga
    24841 ggcgtggatt aaattatgaa tcgctctctt tccccgatta gctaaaatag aagagtcaac
    24901 tgaaaaatca ccgcgaatag tgcccatttc cgctaaggcc ggagtactat tacccaccat
    24961 ttttcttacc atatccacgg catgaatacc ttgtacaatc atttttacca ttgggccaga
    25021 agtcatgtaa tccatcaacc acccacgaac tatttttcct atttccattg gatcatctgt
    25081 tcctacttct tctttagtgt cataaccgta cttttcataa gtggccaatg ttttttctcc
    25141 caatctttta atccacgcgt catttttagg ataatgttcg tctatttgat cttttgttgc
    25201 ccatatcatt ttcatcccca caatttttaa tcctcgctgt tcaattcgag aaacaatttc
    25261 tccaaccaat ccccttttta ctccatcggg tttaatcaaa acaaatgttt tttcttcttg
    25321 aggattcttc atatgattta taattttatt ttccccttta aattaaattc gactttattt
    25381 ttttgttgaa tgatcgattg taagggtatg tctaaatctg taaaataacc gctttctaaa
    25441 agcgcatccc ggaacttaat catttctgat tgagaagttg tccacccatt aaaagaaaaa
    25501 ttgttttcgg caccgggaat aatcaagctt ttaaaatgaa tattttcggc tgcttgttgc
    25561 cacgcttcaa aaacaggcgc ccaatcttta acaaactttt ttgcctctat tgcttgggca
    25621 atatttgcat taaattcttt cgattgttga gaaagttttt ctagttgtgc tctatcaaat
    25681 tggatagaaa gtagctcctt gtccttcatc acgttggttt tgctatagga taaaaaaatg
    25741 tttaaaccaa tagaaataag cagtaaaacc acccctactt ccacaataat ttttagccac
    25801 agtccaagaa aattggttgt tcgactacgc cgatattctt cttcggttcc aaccgccatt
    25861 aaactcacgc caacatcatc aacgcgagga attaaaccac gtaaagcagc gccagtcgca
    25921 aagtaataac ccggcgtaat atcttttatt ttcgaaatag gattttcaac atttaaatta
    25981 tatctatctc gaatcaaatt actcaaatca tcaaaatgaa gcggggtaac taaaataact
    26041 ttttcaatag attcttgaaa tcgtgtaaaa taaaaagtaa taactttttg caaattatca
    26101 tcaaaaattt taataaaatt ttctttactt ataattcctt tctgtaattc tgacgaatca
    26161 agacctaaaa cattaaaagc gtcctgccat gtttgaaaga agttaaactc taaactattt
    26221 ttactaccca aaataaattc taaaccatta gctgaaatat aaataccaag ataatttttt
    26281 tctaaattat caaaaatatt ataatgagaa aaaaatctta acaaactcaa accattaaat
    26341 tctacggcta atggcataat cccaacctcg tcaagaactt ttacatacgc atccacaatg
    26401 gttttttcgg caaagacaga aaaaactctt aacttaaaag agtcactttt gatgggttta
    26461 tctattaatt cccaatcaga atatactcga tcaaagggaa tgggcgaaag cattcgtgta
    26521 ttaagctcaa ctgcagataa aatagagtta cgatctactg tcggtaaatc aaatatttta
    26581 taataaacta tattactttc taacgataaa atcgtataaa gattttttat ttttttaaga
    26641 gcgggctgac ggcgtaaatt taataaaacc tcttttaatg cttccggttt tttcagctcg
    26701 ccctttatta ccgttcccgg ctcaagatct gattcaaaac ttaaaataat tttatcagtt
    26761 ttataaggat caaataataa aaaatgaagg gcggtatcag taatttctaa accgccgata
    26821 tattctttgg caataagatg tttatgaatt ttggaaggtg gagaaatttt catattactt
    26881 cttattatta aatcgcgaat taaatttctt gtaaagagaa aagcgctgct tctcctgttt
    26941 ctgcatcaat actaatgact gcctgcagct ttctctgacg atttaaaaca gtgccggtag
    27001 aaacaatcgt ggcaatgcct atttcattag tgacaacaac gtttacattg gattttccta
    27061 ctgttaaatt ataacccgga gatgaataat ctttattccg aataattttt aaaaaagcat
    27121 cattaacgcc cgaacgagca ccataaaaag cctcttgcga taaacgagct ccataactag
    27181 ctgaatttgt taaatacata acaaaactca aggccactcc cacttcagcc acaataattc
    27241 ctgcaattaa aactactggc agcaaagaaa atccttttct tttttgaaac tcaaaaaatt
    27301 tcttcattag ggtgagtaag taattatgac ctttgaaaca accggcactg ctcctcctgg
    27361 ttctgggtat aaataaacaa tatagcgaaa atagcgctga ttgttatgat tatttaatgt
    27421 aatagacgca atttgatcca ttccagcctc ataataactt gaagttgtgc catccggacc
    27481 aataaaattc caggtggcgg gagtactagt attgcttgtt gctaattgaa atcgaacgtg
    27541 agctccagtt ggcaaagcac ccttccaccg aattccatta aaagcaactc cttcggcaaa
    27601 tgtcgtatca aagacggggg aagttaaaaa gccatctgta gatgttgaca gagtgatcgt
    27661 gccattaata attgaaattg gtccattggt tgaggcataa taaccggctg atttaacaaa
    27721 aactccactg ctaattcgtc cgacatccca gttataatct gcaaaatagt tagaggtaac
    27781 cctagttcga tactccgaca tgactactga attattagga ggccagttga caacaatggt
    27841 tatcttttgg gttgatggat cttcggtgcc acctgtagtt gtaatatttc ctgatgaatc
    27901 tcgattaacg ttttctacat aaaaataata agtataatca actccttcta cgatttttaa
    27961 tttttctccg cttacgatct gccaactacc atctacataa tcagggtaaa aatgattatc
    28021 actccctttg ctgcccggac aaattccatt gggtaaacaa taaaatgaaa cccaactact
    28081 ttccgaaatt gcctttattc cttccatggc atcctgaata agtcctacgg ccacggtcgt
    28141 ttctcttact ttattacctg ttattaccga agttccaata gtggcggcaa taccaccaat
    28201 aataataccc gctagcatta cggctactaa aatttctgct aatacctgcc ctctttcagg
    28261 ccaataaaat tcttttttca catcatcttt agtaatccaa gacttcatta cttcttatta
    28321 taacaaaaac aaaataaatt agtaattgat ttgacctatt ctatttactt caatggtgct
    28381 tgaatattta ggctcaataa cagagcgtat aacaacagta aaagtggcac tattgctcaa
    28441 attgccagtg gatctcaaaa aaacaatatc gctacttgaa ttaaccggtg gaactacaaa
    28501 ttccacttgt tttgataaaa aatgcgtgtc tacaatagca ctagtactaa aagttaatcc
    28561 tctaaaaaga ttatagtaat cgctatcgct tgcgacattt acaaaatgga cgccccacga
    28621 cgaatcgtct tgaccagcca aagcacgatt ccgtgcttca cgtaaatacg ctaccatttg
    28681 ttctgcttca agatcaacac tcaattcttc tcggtgaccc gataaattag caaaacttat
    28741 caccgcaatc attgcaataa tcaccaatga aataattaat tcaataaggg tgaatcctct
    28801 gctattgttt gagaataata atttcataac aaattacatt ccgcctaatt gattagtaaa
    28861 ttgataaaca ggtaaaataa cagccaaagc aatgccgcca acaataacac caataaacaa
    28921 aagcattatt ggttcaaaaa tggctactaa agattttact gccgcatcaa tttcagtatc
    28981 ataaaaatca gccaatgttt ttaaaacttc gtcagttcgt cccgccttct cgccaataga
    29041 aataagggtt cgaatagtgt ggggaaatat ctcttctttt cgaaaggcat ctcccaggct
    29101 aattcccttg gcaacgcctt cgttggcaat acgcaataat gcttgacgca tttcttcatt
    29161 tcctactgtc gtagctgtaa tttccaagtt ctccaaaata ggcaaaccag cgcgcattaa
    29221 agttccaaat gttgtggcaa aacgctgata agccattttt gataatacag ttttaataac
    29281 gggtaatcga aaagctattc tttgaaaaaa tgctcgaccc gttaaactca ttttataaaa
    29341 cattaccgcc gctacaataa gcaatacaag accaattaaa agataaagac cataattaga
    29401 aaggaaaaat gaaacatcaa aaataatttt agtaataggc ggcaagggca tatcgcctcc
    29461 ttgaaaaagt gttgccattt taggaattgc aaaggtcact aataaggcaa taataccaat
    29521 cgaagccact actagtaaaa ttggataaat taatgctgat ttaatacgct gttgcaaatc
    29581 cctttgtttt tcaagcgtta cacttaattc ttcaaaaact gtttctaagt tacccgaagc
    29641 ttcgcccgct cgaatcaagt ttgtaaaaac cgatgaaaat attttagggt atttagcaaa
    29701 ggtggtataa aaaggctgcc cttttgataa gttctctcta atttcaatta acagagagcg
    29761 cactgctggt ttttcgtaat cagcgattaa aatatcaatg gctttaaata tatctgttcc
    29821 cacttttaac attaaggcta aatcacgggt caaaaaaatt tgatcgttaa tgttaatagg
    29881 agaaccgaat aatcgcgttt ttaattttga tttagtagca actagctcta aattaatggg
    29941 attaagatct tgagatttta agtattctaa aacagcgtct tcgttgataa aatcgccttc
    30001 tccttcaata atttttccat ctttagtaga agctcgatat ttaaataaca tatcttttat
    30061 tgtaacttat tctcgaataa ctctcaacac ttcttcaata gtagttattc ctagttgcgc
    30121 tttgcggatg ccatcttcaa gcattgttat cattcctttt tgtttggcta gattttttaa
    30181 aacatctaaa gaaaaatcgg gcttgttaac taaccttctt aaatcttcat ctaaattcaa
    30241 tacttcaaaa atacccacct gaccacgata accgctataa ttacaaatag ggcaaccagc
    30301 tccacgatat aaaattttag gcacttcgat ttcttctcct ccaatatcct ttacctgtct
    30361 tttgagagat tcgatcattt ctggttcagg attataagaa acgatgcaat ccttacaaat
    30421 gcgccttacc aaacgctggg caatcaccgt attcaaaacc gctgccacta aaaacgctgg
    30481 aacgttcata tcaataagtc gcggcactgc cgttggcgca tcattagtat gaagcgaaga
    30541 taacacgaga tgaccggtta aagcagaatg aacggcaatt tcggctgttt cttcatcgcg
    30601 aatttcacca atcataatga tattagggtc ttgacgtaaa attgcccgta atcctgtagc
    30661 aaaggtgatg ccagcttgag gattaatctg agtttgattt acgtaacgaa tataatactc
    30721 tataggatct tcaatggtaa caatattaac gtcgggttta tttaacatgt ttaaaatgga
    30781 gtacaaagta gttgtcttac ctgaccctgt tggaccagtt accagaagca taccataagt
    30841 ttttttaata ctatcctcaa ctattagaac tgtatcctct agcattccta attctcgcaa
    30901 actcattggc ttggccgtag cgcgcaataa acgcatctct actttttcac catagaacgt
    30961 gggcattatt gaaacacgaa tatccattgc ttcgcctagt gcctcatagc gcaagcgtcc
    31021 atcttgcggt tttcgatgtt catcaatttt taaacctgcc aaaattttaa ttctagccac
    31081 aattgccggc tgtacctctt tggggatgcg caacatttca tgaagcacac catcaatgcg
    31141 gaatcgaatt agaacaacgt cttctaagac ttcgatgtga atatcagaag catttaaaga
    31201 aatggcatag gccaaaatag catcaacgat attcacaata gggatttcct cggctacttc
    31261 tttttctccc atcattttta accgcgcggt ctctttcaaa ttgtcttcaa taactttctt
    31321 aaaatcttct actacttcct ttccataaag tgaatacaca taatttaaat ctttggcagt
    31381 ggttaaaaaa actttgagtg gcgcattgac atatttttga acaaaattaa ttgtttctaa
    31441 atcacccgga tcttccatcg ctaaataaac aaacccttta tcgtctttat caaaaacagc
    31501 tacacgccga agacgagcag tttcttctgg taaaagtgaa agaatcgatt ttttgattaa
    31561 acggccgcgt aatttaataa acggcgtttt aaaataatca gaataaaatt gataaacata
    31621 atcttcggaa ataaaaccac gagaaataag aatgtctacg aggtcttgac cggtgcgttg
    31681 agcatcttct tgtgccaatt gaaaagactc aagagaaact aatcccgtat caactaaaaa
    31741 ctccttgagc ttgtctattg gtaaacgtag aggcatatta attaaaaact gattttaata
    31801 agaaaatcgc caataattgg aagtgttgca tatgttcctt gaaataaaaa taaaataatg
    31861 gctaagggca accaaagata ataaagcggc aaacgttctc caaaattaat ttttttaaaa
    31921 atcagtcgca aaaaataaag aatcagacca ataataaaaa tggcaaaaat atacactaac
    31981 cacagcggat gccctactaa cattatagca taaaaaaaca tccaaggctc ttctttttca
    32041 aaaaatcgtt caccggataa acgattagca attttggtta aaaagaaaaa aagcaatcca
    32101 atgagaatag gaaaaagagt cggataaata atacgataat ctatgtaatg aaaaaagaat
    32161 tgatttaatt gctgatgagg cggcaacaga aacggcccca catcagaatt tttaaaaaca
    32221 aaatactgat taataatttt tactaccatc tcccccactt ttaaaaccaa aatcaaaatt
    32281 acagccggtt tgatgaattg ctttattttt ttaaaagcga tgatcaaacc gactgtaata
    32341 agaaaataga ttgcgggtag aaatgtaaca gtttgactta aaaacagcat aattaaatct
    32401 ttctgagaaa atattctatc gtttaaatta aaaaagcatg gccgatccta cttcataaaa
    32461 atctgcttga ttgcccccgt ctttactttc aacatcagtc gtgccagcgc cattactata
    32521 gtaagaagac tccatattac agagtaacgt aaaagtagac gaagccgcat tggatttaaa
    32581 gtagtacgcg taagtagttg tattaacgct cgtggtagca tttttggggt ctaaaggaat
    32641 agtagaaaga ggagagcctg aagaaatctt cgtaaaatca atgctcgtcc aattgccact
    32701 agggctttgg gatgtgctgc ctatagagct ggtgcatgtc cctatagtcg gcgcgccaac
    32761 ttgacttgct ctacaagaag tatcggtaaa taattgctga tctgtaagat ataaattaag
    32821 cgcggttctt aaggcgtcta aatcagaaat acgcttggta tcacgagttt tctttaacat
    32881 ttctgctgga ttaatagtca ccactaccac actcattaag actgctaaaa tggcaatgac
    32941 taccagaagt tcaattagag taaatccttt tttcataaaa ttttagttat atttggatta
    33001 ttaattttaa atttgaaatt ttgtttacga ccttttatta attaattttt gctaactttt
    33061 tagttaattg aaaagcgtta aatcagatcc aatttcataa acagtagcag aattgccgcc
    33121 atcagaagac attaaaccac tattactatt agcttctaat tttgcattca attcatacat
    33181 agttttggtg gaggaaacag taaaagcgta aaaataggga ttggagttgg tgggatcaac
    33241 gggtaaagaa gaaagtggag atctgatact caaacttgtg aaattaagtg ggatccatcc
    33301 gttaccatca accttgcggg gatctgccac gcattgagtt gctgttttgc cgccataaga
    33361 cgcaacagta caaggtgaac ccgacaaaga aagataggct ttagtattgt caaggcttgt
    33421 aaagtcctta tcttcgctta aatataaatt gagtgcagtt cttaaagcat ccaaatcaga
    33481 aactcttctt gaatcccgag atttttttaa catttccgca ggattaattg ttataaccac
    33541 gacgctcatt aaaaccgcta aaattgcaat aacgactaat agttcgatga gagtaaatcc
    33601 ttttgtgggt tgactgaaat aattttttgg gttaaccatc gttctataaa ttaaatctaa
    33661 aatagacctt ttattttttt tgaagaaatt ctttaatttt taatattaaa tcgtcaatgg
    33721 aaattttggc tttaacaaaa tattccacgg cgcctaattg cttccctctt tgaatatcag
    33781 ctgtttgacc taaattggat aaaataatta cgggtttatt acgcaagctc ggatttaaag
    33841 aaatatcttc caaaacttca aagccgcttt ttttaggcaa aattaaatca agcagaatca
    33901 aatcaggttc tatctctttt aattttttta aagcctcttc tccatctttt gcttgtataa
    33961 cctcaaattt atctttttct aatcttaatt tcaataaatt agctaaaaat gggtcatctt
    34021 cgatgactaa aatttttgag gtgtaattat tagtattatt ctgattttcc atagcttctt
    34081 ttaattataa aataatattt tattaaatca attgtggata tcttttgata taataacaaa
    34141 aaacactgcc gaaaggcagt gttttaaaat aaatttcttc ttcattgctt cgtgccgccc
    34201 catcaaaaaa atgatgaccg acctaaggcc ctatctcttt aacaagatag gcaagtatcc
    34261 tttaaagatg atccaccgac agcttccgct accggtgcct tgttacgact tcgtccctgt
    34321 taccgagccc agcttaatcc cgcccttttg gcactagaca attataatat tgttgtaatg
    34381 cccaaagggc gggacttcgg ctgttcccgg ctcccttgac gtgacgggca gtgagtacag
    34441 gactcgagaa cgtattcacc gcggcatggc tgatccgcga ttactagcga ttccggcttc
    34501 atgagggcga gttgcagccc tcaatccgaa ctggggccgg atttttggga ttagctccac
    34561 cttgcggttt ggcaaccctt tgttccggcc attgtatcat gagtgtcgcc caggacatca
    34621 gaaggccatg ctgatttaac gtcatcccct ccttcctccc ccgcctaaac tttcaaactc
    34681 aaacatataa aattttgaaa tttgaaaact taggcggggg cagtttcgca tgacagattt
    34741 aacatgcgat aggggttgcg ctcgttgccc cacttaaggg aacaactcaa gccacgagct
    34801 gacgataacc atgcagcacc tgtgctggcg tccttgaaaa gacggccatc ctttcggaca
    34861 accttcacca gcatttcaag ccctggtaag gttcttcgtt ttgtgtcgaa ttaaacctca
    34921 tgatccaccg cttgtgcgag tccccgtcta ttcctttgag ttttagcctt gcggccgtac
    34981 ttcccaggcg gccgacttaa cgcgttagct tcgccactcc gagggtcgat actcgaaaca
    35041 gctagtcggc atcgtttagg gtgtggacta caagggtatc taatcctttt tgctccccac
    35101 actttcgtgc ctcagtgtca gaaatacccc agtggctcgt tttcacctct ggcgttcctg
    35161 ccgatatcaa cggatttcac ccctacaccg gcagttccag ccacctctgg tactctctag
    35221 cttattcatc tctttggcgc ttcccaagtt gagcttggga ctttaaccaa agatgcaata
    35281 aaccacctac gcacccttta cgcccaataa atccggataa cgcttggggc ctacgtatta
    35341 ccgcggctgc tggcacgtag ttggcagccc cttattcctt aggtaccatc aattagtgca
    35401 aaacactaat tttttcccta agaaaagaag tttacacccc gaagggcttc ttccttcacg
    35461 cggcgtcgct cggtcaggct tgcgcccatt gccgaatatt ctcgactgct gcctcccgta
    35521 ggagtgggac ccgtatctca gtgtccctgt tggggaacag cctctcagct cccctacccg
    35581 tcataggctt ggtgggccat tacctcacca actacctgat aggacctaga cccatcagag
    35641 agcgaattgc ttctttatcc aattcttgaa aagaattgga gccattggga attacctcat
    35701 ctttcgataa gctatgccca actctccggt aggtatcaag gtattactaa cccgtctgcc
    35761 actcctcttg tgcaagcaca agaagcgttc gacttgcatg ccttatccac gccgccagcg
    35821 ttcatcctgg accaggatca aatcctcaga aaaaataaag tctaactctg attaatcaga
    35881 attaaactta gaaaatctaa ttaaatatta gattataaac tcaaaagacg acacgaaaca
    35941 atgaagaaaa aatttatttt ttcattcgct attttgtttt aaaagaacac cgttaaagat
    36001 aaatgattat ctctaacttt atcaatttta ttaaaaatta atccgctgtc aagcaatata
    36061 atattagaat tacatcaaaa aattacaata tccaatattt ttatgtttta cttggaagtt
    36121 tgttgctaat agacaatcaa aaaattgata taatctttta aagaagagca ggtgaaagca
    36181 agacgccgag aaaaattttc aaagaaaatt tttctcggcg tctttaacat ctaaatatta
    36241 ttgactcaac aacaaactaa cgttttgacc attgtggagc gcgacgggct ttcttcaatc
    36301 cgtatttctt tctttctacc attctagcat cgcgtgttaa ataaccagct cgacgcaaac
    36361 gttttttgaa atcgggatta aaagaaacca gagcacgact aatgcccaaa cgcactgctt
    36421 cagcctgggc agataatcca ccgccaacaa catgagctgt taaaccaaac tttccattgc
    36481 atttcatttt ttcaaaaggc gcttctactg ttgcttgcca ttttttcaaa ggaaaataaa
    36541 ctttgtaatc cttgccatta attataatat ctttgttttt tgtatataaa cgcacccgcg
    36601 ccgtagctgt tttacgcgat ccaactcctt cataataaac ctttgttttt ttatcattct
    36661 ctttttcttc tttttctaat ttctcttctt ttaattcctc agcggctcca atatccattt
    36721 tgacaaccgc gttttcagac gcaatttctg cttgtttttt ttctttttcc tttttaacaa
    36781 ctgcttttgt tggttttttt tcagaaatgg ttgatttttt tctaggcata aagttttaaa
    36841 tttttaattc tctttgacct taggctgttt ttaggcaaca ttcccaaaat tgcttttttt
    36901 agaacttcag ttggattttt ttcaaaaata ttcttcattg attttttctt caaatgaccg
    36961 ctatatcctg tatgatgata gtaaattttt tgctctaatt ttttcccggt taatttcatt
    37021 ttggaaacat tttctacaat aactttaaca tcagccactt tattaggctg atagtctggc
    37081 tcatccttgc cttgtaaaat taaagagatt tctgaagcaa ggtgtcctaa tgatttatta
    37141 gtagcatcaa ttttgtattc acgcatattt ttataaaaca aactctacaa cggcaactgg
    37201 tgctccatct cgctttcgac gacgcgggct tttcacaatt ctgatatagc caccttttcg
    37261 attttttaat tgaggtgcaa tattataaaa aagttctttt gccgattttt ccccaatacg
    37321 actgataagc agtcgataag ctgatagtgt ttgtttttta gctaacgtaa ctaatttttc
    37381 tgtttgaact tttaattcgc gtgctcgagc ctctgttgtt tcaatttttt tattaataat
    37441 aagattatta attaaactct tcataaagag ctttctatca ccacgcgttc taccaaattt
    37501 tctgcctttg tttaaatgtc tcataattat tttttagttt ttttagattt cgctggtttc
    37561 tttttggttt ctttctcttt agtttttttc ttaacaactt ctttttcttt tggttccttt
    37621 tcttccaaaa gaggaatgtc ggatattatt ctataatgtt cttccaaaat attagcggct
    37681 tgttttaagg ccattcgtgg caaaatactt ccgtctgtct caataaaaat tctcaaacga
    37741 ttataatccg ttctttctcc gacacgcata ttctctactt caaaattcac ttttttaata
    37801 ggcgtaaatg tagcatctaa actaatggtt ccgatgggta atttttcttt ttgtctcgcc
    37861 tcagcaggca cataacccac cccttcttca acggttaaaa ttaattctag ttctgctttt
    37921 ttatcagtta aagttgcgat atgagtatcg ggcgtcaaaa tttccacatt agaatttttt
    37981 tctatatctc tagctttcac ctcaccctcg cctttggctt ttaaaattag cgtatgcggt
    38041 ccttccgaaa aacacttaac cctgattttt ttaagattaa ggatgatttg aataacatct
    38101 tctaaaacac catcaatgac tgaaaattcg tgaggaacgc ctttaatctg aacggtagta
    38161 atagcaaagc cttttaaaga agaaagaagg actcttctta aggcattgcc aatggtgatg
    38221 ccataacctg tatagagatt ttctatttca aaaactcctt ctgtttctgt ttccttgatg
    38281 atttttggtt tattaggaaa agtaaaacct gtcataaatt ttttagtgcg aataaaactc
    38341 gactatttgt ctaaaatcaa atggaggcat ttgtttagcc aagctaggtt cggcaacaac
    38401 ttttgcctct aatttttccg gatttaaact gagataatcc gggcattgga ccttttttaa
    38461 atttttagaa agatttttaa taggaacttt agataagctt tggggtctga tagtaattac
    38521 atcgcccttt tttaactcat aagctggaac atcaattttt ttattattta caagaatatg
    38581 tccgtggcta accatttgtc gcgccgcttg ccgcgatggt gcaaatccta aacgaaaaat
    38641 tacattatct aatcttaact ccaatttttg aaaaaacgta tcactggttg attctgggct
    38701 ttttgttgct tctctaaaat attttctcat ctgcgcttca ttaacgccat aagtgagttt
    38761 gacttttcgc atttcattta attgacgacc gtattctgaa gcggttttcg cttttctttt
    38821 tttatgcata cctggagcat aaggctttct aataagggcg cattttattg aatagcatcg
    38881 ctcgcctttt aagaaaagct tttctcccgc ccggcgacat tttcgacatt gagcatctaa
    38941 agccatatta ttaataatta aactcttctc acttttggcg gccgacaacc accatgagga
    39001 actggagtaa tatctttaat actcacaatg ttaaactctt gagctattaa actgcgcaag
    39061 gctgcctcac gaccagcgcc aagccctttt aaataaacct cgaattcaga aatatgtaat
    39121 ttcttaattt tttgagcaat tgattctgcc actttggcag cagcaaaaga agtcgctttt
    39181 ttgggacctt taaactgaag atggccagcc gaagaccacg ccaaaacatt gccttcctta
    39241 tcggccactg taattaaggt attattatat gaagaagtaa tataaatttt ggcagaagta
    39301 taagcgccct ttttctcaat ttctttcgcc tctgtctttt tcattgctga ctccaaaact
    39361 tctttttctt ttaatacttc ttcttcattt tgaacaataa ctcgtttctt tcccatatta
    39421 tttaagctct aacttacgtt taccactacc gaccgttcgc ctaacattac ccctgactgt
    39481 tcgtgaatta gtttttgttc tttgaccacg cacaggtaat ttacgagcat gacgaatacc
    39541 tcgataagca ttaatatctt tgagacgacg cacattagat tgaatttctc ttcttaaatc
    39601 accttctatt ttataattat tatcaatgat ttcttgtaaa cgactaattt ccattgcggt
    39661 cagatctttt gttttctttt gaatatcaat gttagctgtt tttaaaattt ttttagccaa
    39721 agattggcct attccataaa tataagaaag gcttatataa atagatttat tatcgggaat
    39781 attaacatta caaattctta ccatagttta accttgtctt tgtttatgct tcggattctt
    39841 tttgcaaata ataaaaacgc ggtttttgcg tttaaccact ttgcaatcac gacatatttt
    39901 tttgactgat gcacgaacct tcataatatt attttctata tacaattcgg cctcttttgt
    39961 catcatacgg actcatttca acgaccactc gatcgcctac taaaatgcga ataaaattca
    40021 aacgcagttt gccagctaaa tgcgccaaaa tttctcggcc atcatctaat cgcactttaa
    40081 aagtagtact aggtagcgcc tcaattacaa cgccttcgta ttgattttgc ttcataaaaa
    40141 aattactgca aaccaaattg taaatatatt tgtattataa aaatcttttt tcattagtca
    40201 actctcgatt gtcctaaacc gcctgtccat ctaaacttat ttttattctt gcactattaa
    40261 gaggaagaaa ttctaaaaat ttcggccaaa tatcaacatt gatattttca atatttttgt
    40321 attgctcaaa tagcttttcc aattctttag tacttttccc tacaagttga tctctaaatt
    40381 gatttacatc aacaggatca cgaaaaacaa gattcgctaa tacaggaaaa gataataatt
    40441 ttctatcact aaaatcaact cgcggaacgc catattcaaa atgatagcta aataattctt
    40501 tttcgcttaa agaattgtcg tctttgatgg ctacagccgt aaacaaatgc ttaatatcac
    40561 tctcttgaaa agctaaaacc ttcaatgcca ctttagcagt catttcaaat tcttctgctt
    40621 tttcacctaa cgcaggtgag gttgtggcag aaacaacaat taattgtttg gcatcatcta
    40681 aaacaatact tccagctggt aattttgttt gcagttcttc attaagagaa acataagcga
    40741 tgtctgcaat tttctttttg gcattggtga tatctgtttg ccccactact tttgtttgac
    40801 caatataacc gccagtcatt ggttttaagg attcgccgta aaatttttca tagcgcggcg
    40861 ttccttgaaa tccaggaata gtaaatctgg ttggtccaat attgtaactc tcgccaggtc
    40921 gatcagcaac tacttcggcg tcaatgtatg aaggagcaat tttcccgttt tctattttgg
    40981 ctccgggcac aactacttta ttgaccaaac gaaaaatctt tccatcgggt gccataaagc
    41041 gcgttgtagc cacgagcgct tgagggcttg agctataagc attataaatt ctaattgttc
    41101 ctttagcctt tgcttcaata tttttaattt cggtggcagg aaatttttga gtaattgtcc
    41161 ggctactttc gaaataaaac aatggtatct gaaaattatt ataatccacc tcttgaatat
    41221 tgctcgtcgc taaaactggc aattgttctt cccactcata ttttttagaa ataatatcta
    41281 cttctgcctt ggctaaaaaa ttgataaaaa ggtaagatgc tataaaaaat aaaagaataa
    41341 ttcccaaagt aatccaaact cctttgccaa aatgaatttt tttatgttct ttcttccgtg
    41401 cttttcttaa ttctttttca gaaatctcta tttccgaaaa agtctcttta tcaaaataat
    41461 cggtaaaaaa ttttttctct ttggggggat ttatttcttc gtcgggtttc gttttgtttt
    41521 ttgacccctc ttgcatttcc actccatcaa aaaccgattc tgtcattttc ttagacttac
    41581 ttatcttatt tatctgaggc ggacgaatat cccctattaa aattttgttt ttaatgcttg
    41641 gccgactaat ttgcgacaac tctgcaattt ttattcctga ttctttagcc attaacgaca
    41701 cctcttcggc atcgctatta atataaatct ttttattagc tgcatcagcc tcacgtttaa
    41761 ttaaacggaa gttcaaaata gattcgctaa ttctagcatt ttcaggaacg atcaaaataa
    41821 c
//
LOCUS       DAOSMC010000004        37999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_1449_length_37999_cov_20.970167, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000004 DAOSMC010000000
VERSION     DAOSMC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 37999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37999
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_37999_cov_20.970167"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..596
                     /gene="ruvC"
                     /locus_tag="PL075_00825"
     CDS             111..596
                     /gene="ruvC"
                     /locus_tag="PL075_00825"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOD96779.1"
                     /translation="MKILGFDPGTKRLGYGIIRPTKNTFEVLTYGCFEPKALKEEKIL
                     EEIFGKTNQLIKKYKPNLVAIEKIFFFHNQKTAFQISHVRGVLLLASSQNNIPICQPT
                     PLEIKTCLTGYGRAEKKDVGFMVKKILKLNKIPDPDDIGDALACALTAFYFQKQRSIS
                     K"
     gene            719..2089
                     /gene="dnaA"
                     /locus_tag="PL075_00830"
     CDS             719..2089
                     /gene="dnaA"
                     /locus_tag="PL075_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOD96780.1"
                     /translation="MDNKELWQSVLGELELQISKPNFQTWFKNSELLEKGGDYVVIGL
                     DSAFAKEWVETKYDKLILKIIRNFEPEIKEVRYQIQSSNKPAVIAIKKKVEVPQETES
                     LNISEYNIDPRTNLNSRYTFQNFVVGSNNELAYSAAMAVVKELGKKYNPLFIYGGVGL
                     GKTHLIQATGNELRKNYKNKIGIRYVSTETFVNEVINGIKNKRMEDVKNKYRKVDVLI
                     IDDIQFIAGKEKTQEEFFHTFNTLYENNKQIIISSDRSPYLLSTLEERLRSRFEGGMV
                     ADISYPDFETRMAILKLKLQNTHSDFYISDEILGLIAEKFPYNIRELEGALTRLLGIL
                     KIRKLNPTRQNIESLLDEITSQRVMIIAPEVVIKIVSEFYNIPEKDLLSHSRKKELVK
                     PRQIIMYILREYLKYSFPTIARKLGDRDHTTVLHAYNKISQQLNKDHLLTQEINLIKE
                     KIYNKH"
     gene            2257..3357
                     /gene="dnaN"
                     /locus_tag="PL075_00835"
     CDS             2257..3357
                     /gene="dnaN"
                     /locus_tag="PL075_00835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOD96781.1"
                     /translation="MKIIVMRENLKKAFSDVEGGIGNGVILPILKNILISTEKGRIKM
                     CATDLEVAITSWVNGKIVDEGGITIPAGVFGAVLNNITEPKLELDSTDSKLKIKSDKS
                     ENIIQGIKDSEFPIIPQIKSDNFIEIKSDLFKKSLNQIINAASPTNRRVELNGILFFL
                     NNELKLVATDTFRLAEKTIKSNEFQTNITELKAIVPLRVIQEFLRICKEDKKIKIYFD
                     PHQILFDLENVQIISRLIEGSFPDYQAIIPKNYMTTVLVNKESLYEAIRLAAIFSSKI
                     NNVNIKLKSPNKIIVFNEESSIGENKSELDAEVEGENQELIFNWHYFLDGLKGIDGEN
                     VLLGFNGDIKPVLIKSTNKDDYFYILMPIKNS"
     gene            3359..3913
                     /locus_tag="PL075_00840"
     CDS             3359..3913
                     /locus_tag="PL075_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOD96782.1"
                     /translation="MTREEAFHLLKNKINDPNMIKHSLAVEACLRELARYFNQNEDQW
                     GLAGLLHDYDYKEMINNPERHGLAAAEELEKLGLTDPVILQAIRAHNEQNHTPRESLL
                     DKAIHAVDPLTGLIVAATLVLPSKKIQDLTVENILKRYREKRFAAGANREIIARCVDL
                     NLSLEQFISICLQAMQKISDNLGL"
     gene            complement(3914..6001)
                     /gene="recG"
                     /locus_tag="PL075_00845"
     CDS             complement(3914..6001)
                     /gene="recG"
                     /locus_tag="PL075_00845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOD96783.1"
                     /translation="MFDFQTPIQLIPGVGPAYLKRFYKLGIKTVGDLLFYFPFRYEDF
                     SQIKKISQLTPDEVATIVGKILQIKTFRTWHKKMFITEAVIEDETGTLKVIWFNQPFL
                     MKNLKTNDWISIAGRLSKEKNGTYFIPSHYEKIYQQDFSNRRETAGLIPVYSETAGLS
                     SRALRFFIKKCLNQTRFPIDPLPLFLRQKYHLLDLKTALHQIHFPTSLINAEAAKKRF
                     HFENLFILELIVTKQRLQLKQYRGFYIKSNMEALKKFTDKLNFQLTQNQKNAILDILK
                     DLQKDKPMNRLLQGDVGSGKTIVAAIAALMTVKNNYQVAFMAPTEILAEQHFRTFTQL
                     FKDFDITIGLITSATTQISWNGLVSILTKKDFLKQCAQQQIQIIIGTHALIQKNVRFP
                     HLGLAIIDEQHRFGVEQRKKLLENKNNFIPHLLSMTATPIPRTLALTLWGDLDISSIK
                     ELPKNRKPIITKIITPPQREKVYQFIRSQVQAGRQVFVICPLINNSLQLEVKSVTQEY
                     EKLKKEVFPDLSIAMLHGKMKTETKDSIMKKFLNQEIDILISTSVIEVGIDVPNATVM
                     MIEGAERFGLAQLYQFRGRVGRGNHQSYCFLFTETSNANSHKRLEAILKAKNSFELAE
                     LDLQMRGPGEFLGKRQSGLPDYFMEALKNLNLIEITKKEAENLLNTDPTLNKYPLLKE
                     KIEQQLSILEMLH"
     gene            complement(6620..6691)
                     /locus_tag="PL075_00850"
     tRNA            complement(6620..6691)
                     /locus_tag="PL075_00850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6656..6658),aa:Arg,seq:gcg)
     gene            complement(6699..7334)
                     /locus_tag="PL075_00855"
     CDS             complement(6699..7334)
                     /locus_tag="PL075_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96784.1"
                     /translation="MSKKLDAIARQKNKPSDNTDVLDLRKAASKNIQEKTRFSNSNEL
                     ASVSFNEKTSEVSPRLSAQKKLQPIWWTIEKKYAINSWFSIIFFAVIVICSIVAIITS
                     NWIFLAILILGALLFVLLFLKSRKTWYRLTPDGFYVDDDFVSYHDIKYYSILETADKN
                     FIIFETDKLFDKHIFLPFKKEKTVKIREFLNNFLKEKEIKPSLMDLLANLF"
     gene            complement(7327..8445)
                     /locus_tag="PL075_00860"
     CDS             complement(7327..8445)
                     /locus_tag="PL075_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOD96785.1"
                     /translation="MVNQNSSSPAIKKRPTWWHHVDVPRLNTNSVVEAKILRKTPRSL
                     YLDLGQYGIGIVWGAEFTRSAKYIEELKEGDVVRVKVLSPENEFGMVDVSLQDNPQDN
                     QYRWIKDLMNNNQSIVGKVVSANRGGLLIKINDLQGFLPTSQMSEKHFPRVEGGDKEK
                     ILEALEELIGQDIEVKIINFNPALGKIIFSEKKIEEDAMQDIIDKLKVGDQVEGSLNK
                     IINGGAIINLKDYPQLKAFLPLEEVSWTPIDKLEDVIKPDQVYRFQIISINKNKKIIL
                     SLRAQKEDPVLKKLQQYQPQQVIKGKVHKFIPFGALIQIEDDVFGFIHSSEFGGIEAM
                     QQKLALNQELEFIIDTVNLNEKRINLRLKENFLNQNNE"
     gene            8690..9328
                     /locus_tag="PL075_00865"
     CDS             8690..9328
                     /locus_tag="PL075_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD96786.1"
                     /translation="MTIEWFGEGCFKITETGNHFSILTEPPTRESGLAVPRHKVDVAL
                     NVFSDLSDSLFSQEKKENFVITNAGDYELKGVFIRGILLSVNKSIIKSAYKIKMEDIN
                     IGYLGMINSKEITPEVGNFFSDTDIVLVPIGGGDVIDAAEAKNIIKQLEPKIVIPMYY
                     KIPGLKIKRAELENFLKKMEVKSVEKTERLLIKSKDLENWGEEMKIFIVNSL"
     gene            9325..9756
                     /locus_tag="PL075_00870"
     CDS             9325..9756
                     /locus_tag="PL075_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96787.1"
                     /translation="MRSFQTIQNKLNQKIQALRSQPYQKRVVIMQVCLMVIICLLFLG
                     WIFSVKNNVAENPVIQKTQEATKEETDKPNRFTIFQKGIELVYQDYLRPFFGKVGQIL
                     SGAFSQFIGLVIDIAQPISYGMNQLITQYKAYVELVLSLVK"
     gene            9763..12285
                     /gene="gyrA"
                     /locus_tag="PL075_00875"
     CDS             9763..12285
                     /gene="gyrA"
                     /locus_tag="PL075_00875"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOD96788.1"
                     /translation="MTKKFKSKRLKKIQPSKIDEKQNESSGLGKSDQIILREITEELK
                     SAYLDYAMSVIISRALPDVRDGLKPVQRRILYAMSEAGFWHSSKFRKSASIVGEVLGK
                     YHPHGDVPVYDALARMAQSFSLRYPLIEGQGNWGSIDGDPPAAMRYTEARLAVLAEEM
                     LKDLDKETVPFQDNYDGTRKEPLVLPARLPNLLINGTSGIAVGMATSIPPHNLGEVCD
                     ALIYLVDHPNAEVKDLLQLIQGPDFPTGGVIYNRQSLEELYASGRGTITVRATVEVQE
                     RKAGQADLVVSDIPYQVNKADLLAKIAELVQDKRIEGIRDIRDESDKEGLQITIQLKN
                     DAHPQKVLNNLYKHTDLQKNFHVNFLALVEGIQPQTLSLKGLLEEFIKHRQAIVYKRS
                     QFDLKKAKDRLHILEGLLKALKNIDAVIKTIKTSKNRDEAKEKLIKQFKLTVIQAEAI
                     LEMKLQTLVGLERQKIEEEAKLKEKEIKELEEILKNPKKILQIIKDETLEIKKKYADD
                     RKTRVLNIPIGEFKEEDLIPDQEVVIMMSQDGYIKAFEPEIIKNQKRGGKGMIGFDVK
                     EEDRIQHVLLANTHDNLLFATSSGRVFQLRAFEIPMATRTARGRSIFNFLDLSKEDKI
                     AAIMVYPNQQSKKSGYLVMVTKNGIMKKMNLTEFANVRRSGLIAMKLKEGDELKDAKI
                     VEPNDEIIVLSAYGQALRFQEKDLRPMGRSAAGVLGIRLKTEKDNEVRGFDVIKPELK
                     DGMLLVVMEKGFGKRTALKEYRLQHRGGQGIKTAKISEKTGKVVAIKVIAKDTKQVLL
                     ISKKGILIKTDLGNISQQSRIAQGVRIIRLDPDDVVAGIVVL"
     gene            12317..12844
                     /locus_tag="PL075_00880"
     CDS             12317..12844
                     /locus_tag="PL075_00880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOD96789.1"
                     /translation="MAHIFDPKNLVAQLNLAPGSVVADFGSGSGILTLELAKAVGPEG
                     KVFAVDVVSQQLESVKSLAQMRGINNIETRWANLEKTSTLDPNSCDWVIIANLLFQVE
                     KKFQPRIFEEAYKILKTEGKLVIIDWKPESILGPPKENRLTIEEVKEMAQKKQFKFMH
                     TIEIDDTHWCAIFQK"
     gene            12848..14140
                     /locus_tag="PL075_00885"
     CDS             12848..14140
                     /locus_tag="PL075_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOD96790.1"
                     /translation="MPQQELTKSQKIIFIVIAIIILMVVLMLLGVIPGLKSKEKEKTS
                     EMPAITLEFWGIDNPDAFKGVLEGYKAVYPNVTINYQQIDEVGYESKLLNALAAQKGP
                     DVLMIKHNWVPKHLDKLYPSGVLLKDLKAGFVDVVADDLYYQGKVWALPLYVDTLALY
                     YNKDFFNTAGIVMPPVTWDEFVKDAQLLTKKNSSGEILQAGAALGTVNNIKNAVDIVL
                     LLMLHNNQKIVDENGRGIFDTANGVNAVNFYLSFANPASPNYSWSTHFPNATEVFAQN
                     RLAMMIDYSSAISTLNALNAYLNYGIAPLPQPANTITKQNYADYWAFTVSATSKHPQQ
                     AWDFIYYLVDKGAVEYLNSTLRPAANRVLIQACKNDEKLGVFCEQALTAKTWLQFNPE
                     ENTNILKNMLESILDGRATPLTALREAAQRINRSANQF"
     gene            14200..14637
                     /locus_tag="PL075_00890"
     CDS             14200..14637
                     /locus_tag="PL075_00890"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96791.1"
                     /translation="MASFYLSNYALAQQVTQVVPSPVPAPVTVTVEKTGDGLLSGVAT
                     TCREAGQCTFGHILIVITNIFHLLRQIAFYAAIGFGLYGGIRMIVSQGKPEGVKAGQK
                     IIEAAFIGLIIAYGASFIINIVCTILLGYPLTLGNILNANWIR"
     gene            14723..15229
                     /locus_tag="PL075_00895"
     CDS             14723..15229
                     /locus_tag="PL075_00895"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96792.1"
                     /translation="MITSDASAQLVSPIKAKTIGGLMQQIVQWILNLAVPLSAVMTIW
                     GAVLLMTAGGNPQKVKQAQNALGWAMGGLVVVMLIEGLFGFGKDLIANASSLTQLINT
                     IQQYLLLIVGPLSLVMYFYSAFLMSTSNIESIKKAKSILIWTTVAVAIISIFSVANLI
                     NLINQLAK"
     gene            15231..15584
                     /locus_tag="PL075_00900"
     CDS             15231..15584
                     /locus_tag="PL075_00900"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96793.1"
                     /translation="MIIKKFLPGIIMIIVGLSLISPVLAQQNQLATSSIRISGVQDII
                     KIINNIAKWMYTILLALVVIFVLVAAFTFLTAGGKTENVKKARNQIMYAVIALVVALL
                     AFSINTIITNILLAK"
     gene            15633..15986
                     /locus_tag="PL075_00905"
     CDS             15633..15986
                     /locus_tag="PL075_00905"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96794.1"
                     /translation="MKQIITIFLLIASMSFTIGGLVSAQVYTNLQEPPAPRDLHTWMG
                     TVNTIAQWIYTIVLVAAVILGLIAAFSYITAAGDSNKVKTANNMLIYAAIAVVIAILA
                     FSITKIVGGLLGTPI"
     gene            16039..16392
                     /locus_tag="PL075_00910"
     CDS             16039..16392
                     /locus_tag="PL075_00910"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96795.1"
                     /translation="MKKFFAIVSLLAVVSLTFGSVVSAQVYTGPANLPTPQNLPTWME
                     TVNTIARWIYTIVLVAAVILGLIAAFSYITAAGDSNKVKTANNMLIYAAIAVVIAILA
                     FSITKIVGGLLGAPI"
     gene            complement(17007..17090)
                     /locus_tag="PL075_00915"
     tRNA            complement(17007..17090)
                     /locus_tag="PL075_00915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17054..17056),aa:Leu,seq:tag)
     gene            complement(17220..18167)
                     /locus_tag="PL075_00920"
     CDS             complement(17220..18167)
                     /locus_tag="PL075_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96796.1"
                     /translation="MPEILRKPPSQIKIRTLETDVEDMRKSGGGFISGKVLGRTPEEI
                     NRSIHNEGNKNAVTFFPTQTDTSNIAQKKNKVPIIIIATIVGIGIIVGLVFYISHKPQ
                     TSPPIATPSLTPQYSSLLRNFNGVQEWVSFDLTLNGFEDILVKQFYTISEPQKTKELV
                     FVKNNGSLVTASEFFDVLFDHFKGITAAERPQWENRFSFIVFTDNSGSKSIAYVTKID
                     TAKSNPLVLPKLKQDFSRAFENYILNNTELLTYQYLQNPGKPNSPFLPQTIGPLNASV
                     LKFSTGAEFYYAFYGNEYYFLVATSKQAFEELLNLIVVR"
     gene            complement(18228..19397)
                     /locus_tag="PL075_00925"
     CDS             complement(18228..19397)
                     /locus_tag="PL075_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HOD96797.1"
                     /translation="MSLSKKLANYFAASTILAGTILGAGMFSLPFVFINAGSFLFVIF
                     LILVTAICTVTHLAYGESLLRTEGEHRFIGMAEIYLGHWAKTLITITSFISIGGCLLA
                     YLILGGQFLQNFSQIIHYPLNQTQSVLLFWVIGSLGALPGIKFIGKGETISLILIVML
                     IAVFFILGIPQLNLAHVPLINASQLLLPYGVLLFALSGASAVPEIYSYFQKKSLTPKD
                     INFKKPIVWGSVIPALLYLIFCLGAIGLLGVQNIGVDLVPQLISINPLLGIATDLLGI
                     LLILTSYFILSLNFKNSLFFDLHMKQWVAWLIAIFLPLILYQLGAHDFIKVIGFLGAV
                     VLGIESLITFLIHKKAQTNGATQPPFVLKIPNVLRFILVALLILGAALEVLRTIA"
     gene            complement(19384..19845)
                     /locus_tag="PL075_00930"
     CDS             complement(19384..19845)
                     /locus_tag="PL075_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOD96798.1"
                     /translation="MNDLIMLSQLLLSLFLGSLIGLERQLARKPAGFRTYSLVSLSAT
                     VFTIISETLALRYANIPNFSFDPSRIASQIIVGIGFIGAGVIIYQKSKIRGITTAASL
                     WVTAAIGMAVGFKLYTLAILSTCLVLIVLIVFYFFIENRLEKKVAKKDEFI"
     gene            complement(19866..20153)
                     /locus_tag="PL075_00935"
     CDS             complement(19866..20153)
                     /locus_tag="PL075_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HOD96799.1"
                     /translation="MPKKANSQTKEKKEKLIGIVTHYFDKIGVAALKLKAPLSVGDRV
                     LFRSNLGDLFEQDITSIQIEHQSVQKAKKGDEVGIKVDQKVHDGNEVYLVE"
     gene            complement(20205..20771)
                     /locus_tag="PL075_00940"
     CDS             complement(20205..20771)
                     /locus_tag="PL075_00940"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOD96800.1"
                     /translation="MEIFKSKIKIAISGAAETGHCSANAMELSMKLGEELAKHDCIVV
                     TGATTGAPYWVAKAAKQAGGMVIGLSPAISEKEHINKYHLPIDYHDLIIYTGAGYAGR
                     NLLLTRSADAVITICGRIGTLNEFTVAFEENKLTGVLEGTGGIADMIRQIIDKAFRGP
                     GSVVFNSDPKALIEELIENILEEKTNNQ"
     gene            complement(20936..21184)
                     /locus_tag="PL075_00945"
     CDS             complement(20936..21184)
                     /locus_tag="PL075_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96801.1"
                     /translation="MKLLACDLCHSVVPLTKRLKSCRCKNIKGRYLDQKNIIVYVRDK
                     SSCRVLGLSNAVRYGFSKEGAAWIINFNDPTIKKLIEL"
     gene            complement(21294..21366)
                     /locus_tag="PL075_00950"
     tRNA            complement(21294..21366)
                     /locus_tag="PL075_00950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21331..21333),aa:Lys,seq:ctt)
     gene            complement(21499..22170)
                     /locus_tag="PL075_00955"
     CDS             complement(21499..22170)
                     /locus_tag="PL075_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOD96802.1"
                     /translation="MFFYKTFYWLIYSLMRPQLKKVTGLENVPKDRGFLIVANHQNNY
                     DPLIIAVALYPFFQRHLKPKNHKIYFIGSPRLKNEFFRHPFISALLTLGMDSIGYLPA
                     DRSSLKRSAELIQKGNAVVIFPEGGRNPKKVLKRGRRGAAVIALLSGCCIIPIGSFGP
                     RTSGFKEGLLGFFASKNLVIGKPFQFQSQSQREIDANPQILIQATNKIMDKIAEVAQK
                     NINRP"
     gene            complement(22470..22541)
                     /locus_tag="PL075_00960"
     tRNA            complement(22470..22541)
                     /locus_tag="PL075_00960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22506..22508),aa:Arg,seq:tcg)
     gene            22627..24201
                     /locus_tag="PL075_00965"
     CDS             22627..24201
                     /locus_tag="PL075_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOD96803.1"
                     /translation="MKDDQKNFWLKNRINLASPQLEASREMTKPMEIPIEIKNILEQF
                     NKTPQKFEAYLVGGCVRDVLLKKKPKDWDITTNATPQEIQKLFPNSFYANDFGTVSVI
                     TNSDDETLQVVEITPFRLEGKYSDKRHPDEIKFATILEDDLARRDFTINAMAIQVKDG
                     KLKIIDLFNGQKDLKNKLIRAVGDPEERFNEDALRLMRAVRFAAELDFQIEEKTKKAI
                     EKLHSNLECISKERVRDELIKMFHCENAARGIEMLHELKLMPYVIPELEEGVNVVGVT
                     KKLKEHIKYESLTTFEHLVKTLDYAAKNNFNLETRLAALLHDVGKPLTKKGEGLEASF
                     QGHERVSAQMTKEILTRLRFPKKTIDKVVKLVRWHFFFYDINKVTPAGVRRLIRNVGP
                     ENIDDLLKLREADRSGSGLPKISSYRLRHLRYMIEKVKKDPIEPKMLKIKGDDVMKIL
                     NIPPSPKVGWVLNILLEEVLDDPAKNNREYLEKRLKELGKLADEDLKKMAEEAKSKKE
                     EIEEEIDEEMRKKYNV"
     gene            24213..24283
                     /locus_tag="PL075_00970"
     tRNA            24213..24283
                     /locus_tag="PL075_00970"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24245..24247,aa:Pro,seq:cgg)
     gene            24444..25598
                     /locus_tag="PL075_00975"
     CDS             24444..25598
                     /locus_tag="PL075_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96804.1"
                     /translation="MKFKTLFLVFLILLIGVLAIYFSPFQKTNKTNDYNLEEEKANLN
                     EGNGEMTIQETPSMSPEEKTTTLFDFDPHLLSWEEIKPTAIWERRDAQAGVVFQDKIW
                     ILGGLGGTNTKTTDSYSSFPHYKDVWVTEDGENWELVTNDAPWGKRRGHAAVVFQDQI
                     WILGGVTNAEIFKNDVWRSADGKNWVQVTAQAPWLPRKGHAAVVFKDKLWVLGGVSIK
                     GVKNDVWVTEDGENWELVTNDAPWGARYDLAAEVFNDKLFITGGVVPGEWKGMKDVWF
                     SNDGINWELANKESPWLGRHGLCLTSYNNYLWVIGGWSGYGKGYNDVWFSQDGKIWEQ
                     TLEDSLWPGREDLTCVVFQDHIWLMGGMTTNGTRKNDIWRSTISGTQEKM"
     gene            25766..27166
                     /locus_tag="PL075_00980"
     CDS             25766..27166
                     /locus_tag="PL075_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD96805.1"
                     /translation="MVIHQYSKSFTLIELLITLGILGVLMAVVVITINPAEFLKQSRD
                     TKRMSDISNLQTAINLYLFEGSSLGTPGVTYISIPAANSDCSDLNLPAGSWHCATSDN
                     YLKVDGTGWLPLNFSALTISSPLSVLPVDPQNSVSESYYTYSTDGSGFVITAVPESNK
                     YRNNSDLLTGFVASGASDKLVGGNFPNGWVFVPGDPNFKTPGFWVMKYEAKCVSGNTP
                     LTSPDTGYHTYNNSSQPCAGDRYIASTSEGYPITNISHNTAKAYCQSIGAHLLTNDEY
                     MTIVRNAEQVPVNWSNNQVGNGVLPRGNSNSAAAQDGSSIYGTGYTDHTHLRTLTLSN
                     GSVIWDIAGNVYDHVQRSNDNAGDLTNTMVLPACSDAQAAWGWCKYASTESPYVSSWT
                     DDVAKDKVAPSNTSWTSSQNIGQVYTYKNGTNQGTTVFLRGGLWSDGAFVGPFALSLS
                     WGTDGTYNNVGFRCAR"
     gene            27201..27536
                     /gene="avd"
                     /locus_tag="PL075_00985"
     CDS             27201..27536
                     /gene="avd"
                     /locus_tag="PL075_00985"
                     /inference="COORDINATES: protein motif:HMM:NF033474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diversity-generating retroelement protein Avd"
                     /protein_id="HOD96806.1"
                     /translation="MLEDLIVFQKLYDLILWIYPIINKFPQKQRFVLGQQIQNKLLEI
                     LKNIIEGNQAIQKTVILKHASVNLDELRILIRLSKDLHFLSINQYQIAAEKINEIGRF
                     LNAWLRRFS"
     gene            27994..29025
                     /locus_tag="PL075_00990"
     CDS             27994..29025
                     /locus_tag="PL075_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HOD96807.1"
                     /translation="MNKNLFEQVADFKNLHSAYFAARRCKRYRSCVLKFEYNLEENLL
                     TLKKELLNKTYHHGGYHEFIITDSKKRIIKAPLFRDRIVHHALCNIIEPILDRGFIYD
                     SYACRKGKGTHVAVKRLESFLKSCSATQKRRDVISSRVYCLKCDISKYFENVDHEILL
                     ELLEKKIQNGDILWLLREIIGSNAQGIPIGNLTSQLFANVYLNELDHFIKRVLGERYY
                     IRYMDDFLILDTNKWHLRETKERLRMFLCDRLKLELHPKKAEIFPADKGIDFLGYVVR
                     NNKRFLRKSTVKRFLKKKRRYYKTMIKNGQQTEVFLQNVFNSWRGYICFADSYKLIKD
                     IRASFCQPF"
     gene            29537..30409
                     /locus_tag="PL075_00995"
     CDS             29537..30409
                     /locus_tag="PL075_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96808.1"
                     /translation="MDLKDYEAILRRCWSAETSLDSENWSPHNPAWGQCAVTAAVIQD
                     FFGGDLMRGIVEINSGIQPVTTSHYWNRLANGQEVDLTREQFPFSARISVGEIRSRRY
                     IFSNPHTLSRYSILRLKFENELRPNPLFFDEIYRLCFGAAQLSDCSYMQFGCVVFQEG
                     KKQPKVMTSNRFVEPMRFICSSECIRKYIKSEQNLMIGACGHAEEWALKELREKKIHP
                     DRCSFYVAGVKPFIHTPLLREKEGFSCLRCAVQLYMHGVKKIYIPVQDHWASQTPEEA
                     LISSLDFAIGRRQI"
     gene            30543..31526
                     /locus_tag="PL075_01000"
     CDS             30543..31526
                     /locus_tag="PL075_01000"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOD96809.1"
                     /translation="MAKIINGFFSKNVQRAAALIQQGEAVAFPTETVYGLGADVYNKK
                     AVAKIFEIKERPYFDPIIVHVANLKQLEEVAQIKDERVYDLVKHFWPGPLTLVLPKQK
                     KIPYLVTAGLDTVGVRMPANVVALSLIQALGHPIAAPSANLFTRLSPTTAEHVFKQLG
                     PKIKIILDGGKTICGVESTILLINKNIQPVCLRMGGLPIEEIEKVVGKITIPKKQSRK
                     ILAPGNLNFHYAPRKSLIIVGKEEEIWRYPESLKKKIALLAFQKIKNKRKFYEWRILS
                     PRGDLKEAAANFFDFLHQLDEGSAEIIFVEKVKEVGLGRAIMERLNKATHQ"
     gene            31526..31984
                     /locus_tag="PL075_01005"
     CDS             31526..31984
                     /locus_tag="PL075_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOD96810.1"
                     /translation="MNKNIIILIAIILLGLLIFEIYFLRGKYSTGEVIFKRQNIGITV
                     EIASNSLTQMQGLMGRKELPEFSGMLFVFRTEAPRTFWMKNTHIPLDLIFISHDKKIK
                     EIKANFEPCIDSNNCPSYQSKESAQYVLEVNGGFCERHQIEVGDEIELKF"
     gene            32102..33034
                     /locus_tag="PL075_01010"
     CDS             32102..33034
                     /locus_tag="PL075_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOD96811.1"
                     /translation="MLAEKISDHLKNLAKKNPAIKRQFYLSKWELHTKEDKLNSVDPL
                     LEDEHTKKRGLIYKYRGRVLILLTLSCAAYCRFCTRRRCVSEIEKGILSKEDIDNIAQ
                     YIKQHKEIKEIILSGGDPLTVPNLLNYSLKVFSALPQIKIIRIGTRVPVSNPKLVTDN
                     LLNLIRKISQPVYVGIHFEHPDELTPETIKACEKLRKAGAILYSQSVFLKGVNDSYEV
                     LYELFSRLIEIGVKPYYLYRCDPVQGIEHFRVPFEKEIKIATKLRKNLSGLAWPTYVI
                     DTPNGNGKIPVPLEFWQFDKKQFRDFLGRKHEVI"
     gene            33685..35118
                     /locus_tag="PL075_01015"
     CDS             33685..35118
                     /locus_tag="PL075_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96812.1"
                     /translation="MRKLSLVVLIGLVLLTLPVAAQQSQGKEIELKTLGRAPLAPYLQ
                     TEEEVRIVLKKYNEEIGKALEYFPGGALGEIVYLKLYENVDSFVMQLQQIPVGQKMDW
                     MIYRNGGLLKNVRWAGNSSFDAFVFEVEAEGKLFQFAVPIRCGNVSLISMKEIPISAP
                     VVQPQPQPQPKAEQPFVPPAVIQAPAPTPAPALSAPIKAQKRWSPTVKFKVGSGWAKT
                     SMPANQNLEGRVDFFTLIPDEMSLFYCEDGTAAWLYSPTKDIPFTLGEQIGLHTQLQN
                     TSIRKYQPFFAGLELEIAKNFFVTADYFHIGKFHLQEYSLSNLMSVTELRLLGEYDST
                     SGSADGCTPTYVYYIGLERQRREQLIQQDIRIEEWSVGVKFELKLAKKFYVAPAAGVT
                     WQEQRGTREVTEAVTKMYPFKEEALEPQQVVSSAEKIKTSVAHPYVGASVELYCFQVE
                     ARYLMKKFNEEVYTSPWRVQGALVLRF"
     gene            complement(35193..35390)
                     /locus_tag="PL075_01020"
     CDS             complement(35193..35390)
                     /locus_tag="PL075_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOD96813.1"
                     /translation="MGKKKIKINKDKCLGCGSCTFMYPEMFYLDEDGKAEVKELKKID
                     EEKLQSAIDACAAEAIEIEEE"
     gene            complement(35415..36107)
                     /locus_tag="PL075_01025"
     CDS             complement(35415..36107)
                     /locus_tag="PL075_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOD96814.1"
                     /translation="MKKTKKALTKKSKKQKSKKEADIWTKGNPWVYSQTVKEHFLQPK
                     NFMKFGEEEKFQYNGLGMIGSPVCGDVMKFWILVDPKTEKIKDARWRTFGCASAIAST
                     SVLSEMILRHGGMKLEQARKITPNDIIKELGGLPDIKYHCSVLGDKALRDAINDYYRR
                     TGQYDKIEPEGSRVIDKILKITEKDIEKAVLDGAKTLEEVQEKTKVGLGDPSCIPLVE
                     ELIRFYKEKYNL"
     gene            complement(36079..37296)
                     /locus_tag="PL075_01030"
     CDS             complement(36079..37296)
                     /locus_tag="PL075_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOD96815.1"
                     /translation="MKRYYFDYAATTPTRKEVISAMKPFWQKQFGNPSSLYLEGRIAK
                     QAIESARNKIAEILQVQSKEIIFTNGGTESVNLALQGTAHYYQKEFSQPKIIISAIEH
                     PAGLETVKALKEQGFEIAYLSVQKNGLVDLDQLKSLIDDQTILISIMYANNEIGVIEP
                     IPKIAQIIKNFRTEHKTIFPLFHTDACQAAGYLDIRPQKLGVDLMSLNGGKIYGPKAT
                     GVLYKKEGVEIKPLIYGGGQEKNYRSGTENVAGIVGFATALTLAQKEKKQESQRLKLL
                     RNYLIKNVLKNIPKVFVNGDLKNRLPNNAHFTILDVEGEALILKLDEYGIAASTGSAC
                     HSKSLSPSHVLQAIGLPAEFIHGSLRITLGKDTTKKDIDYLIQKLKKVVLELRELSPI
                     SLKFNEKNKESTN"
     gene            complement(37293..37691)
                     /locus_tag="PL075_01035"
     CDS             complement(37293..37691)
                     /locus_tag="PL075_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOD96816.1"
                     /translation="MNFKSIKISKQIELALRWLIYLAQTNKLKFVSLRSFCDQTKTSF
                     YYLQKINRALIKNNFVESKRGKNGGYRLKNNPKNISLLSVIEALEGPLSLINCSSRCN
                     SSHCTLKSCWVGLTEDITLKLSRIKISQLV"
     gene            complement(37791..>37999)
                     /locus_tag="PL075_01040"
     CDS             complement(37791..>37999)
                     /locus_tag="PL075_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96817.1"
                     /translation="YSQKSIQENADKFIAYLNQNGWTIVNNTNSNGIYNIYATKSRNS
                     MSIQMFYEEGGGGLKVQATYAAMK"
BASE COUNT        12301 a         6258 c         7314 g        12126 t
ORIGIN      
        1 aaaaaatttc tccagaagaa aaagaaattt atgaaaaatt atttaatgaa ttagacgaac
       61 aatcagaagt taaagatatt ttttctacta ttgaatttta attttagcat atgaaaattt
      121 tgggttttga tccgggaaca aaacgattag gctatggcat cattcgtcca acaaaaaata
      181 cttttgaagt tttaacatac ggttgttttg agcccaaggc gttaaaagaa gaaaaaattt
      241 tagaagaaat ttttggaaaa actaatcaat tgataaaaaa atataaacca aatctagtag
      301 ccattgaaaa aattttcttt tttcacaatc aaaaaacagc ttttcaaatt agtcacgtca
      361 gaggtgtttt gcttctcgca agcagtcaaa ataatattcc tatttgtcaa ccaacaccat
      421 tagaaattaa aacttgtctc actggttatg gccgcgctga aaaaaaagat gttggtttca
      481 tggtaaaaaa aattcttaaa ttaaataaaa ttcccgatcc agatgatatt ggagatgctt
      541 tggcgtgtgc tctgacagct ttttattttc aaaaacagag gtctatttct aaataataag
      601 cacttgtgga aaaaagagta atccacaggt aatccacatt tgcttatata ctattttaca
      661 aggtatttat ataatactat tgcgttttta aatctttaat tcataattgg atgtgaatat
      721 ggacaataaa gaactttggc aatcggtttt aggggagtta gagcttcaaa tttctaagcc
      781 taattttcag acttggttta aaaatagtga acttttagaa aaaggaggag attatgtagt
      841 gattggcctt gacagtgcct ttgcaaaaga gtgggtggaa acaaaatatg ataaattgat
      901 tttaaaaatt atcagaaatt ttgaaccaga aattaaagaa gtgcgctatc aaatccaatc
      961 tagcaacaaa ccggctgtta ttgctattaa gaagaaagtg gaagtacctc aagaaacaga
     1021 atcgcttaat atttccgaat ataatattga ccctagaacc aatttaaatt ctcgctatac
     1081 atttcaaaat tttgtagtgg gctctaataa cgaactcgct tattcagcag caatggctgt
     1141 tgtaaaagaa ttggggaaaa aatataatcc gctctttatt tatggcgggg tgggtttagg
     1201 aaaaactcat ttaattcaag caacgggtaa cgaactaaga aaaaattata aaaataaaat
     1261 tggtatccga tatgtttcca cagagacttt tgttaatgaa gtgattaatg gaattaaaaa
     1321 caaaagaatg gaagatgtaa aaaataaata tcgtaaggtg gatgttttaa ttattgatga
     1381 tattcaattt attgctggta aagaaaaaac tcaggaagaa tttttccata cttttaatac
     1441 attatatgaa aacaacaaac agatcattat ttcttctgat cgatcgccat atttattatc
     1501 cactctagaa gaacgattgc gttctcgttt tgaaggaggt atggtggctg atattagcta
     1561 tcctgatttt gaaacgcgaa tggctatttt aaagctaaaa ttacaaaata cacatagtga
     1621 tttttatatt tctgatgaaa ttttaggatt aattgcagaa aaatttccct acaacattag
     1681 agaattggaa ggtgccctta cacgtttatt aggcatttta aaaattagaa aattaaaccc
     1741 cactcgtcaa aatatagaaa gtcttttaga tgaaattact agccagcgcg taatgattat
     1801 tgcgccagaa gtagtgataa aaattgtcag tgaattttat aacattccag aaaaagattt
     1861 attaagtcat tcgcgtaaaa aagaattggt aaaacctcgc cagattatca tgtatatttt
     1921 gcgtgaatat ttaaaatatt cctttccgac gattgcacgc aaattaggtg atcgcgatca
     1981 tactactgtt ttacatgctt ataataaaat ctctcaacaa ttaaacaaag atcatctttt
     2041 aactcaagag attaatctta ttaaagaaaa aatttacaat aaacactaat aataaaatag
     2101 ttatcctaaa catttcatca tttttcagct atttatacac aagtaatcca atggtgttaa
     2161 aaaacaaaaa gtgttttata atgtaataaa ataagctata aaatattttt ttaataataa
     2221 aatgtcgtct ctattacaac tacttaatat aaaaatatga aaataatagt tatgagggaa
     2281 aatttaaaaa aggctttttc tgatgttgaa ggaggaattg gtaatggtgt tattcttccc
     2341 atattaaaaa acattttaat cagcactgaa aaggggcgaa ttaaaatgtg tgctacagat
     2401 ttagaggtgg caattacaag ttgggttaat ggaaaaattg tggacgaagg ggggataact
     2461 attcctgctg gtgtttttgg tgcagtttta aataatatta ctgaaccaaa attagagtta
     2521 gattctacag attccaaatt aaaaatcaaa agtgataaat ccgaaaatat tattcaaggt
     2581 ataaaagata gcgagtttcc tattattcct caaattaaat ccgataattt tattgaaatt
     2641 aaaagtgatt tatttaaaaa aagccttaat caaatcatca acgccgcttc tcccactaat
     2701 cggcgagtag aattaaatgg aatccttttc tttttaaata atgaattaaa attagttgct
     2761 accgatactt ttcgattagc agaaaaaact attaaatcta atgagtttca aactaatatt
     2821 actgaattaa aagctattgt accattgaga gtgatccagg agtttctaag aatttgtaag
     2881 gaagataaaa aaataaaaat ttattttgat cctcatcaaa ttttatttga tttagagaat
     2941 gttcaaatta tttcacgttt aatagagggc agttttcctg attatcaagc tattattcct
     3001 aaaaattata tgaccacagt tttggttaat aaagaatctt tatacgaagc tattcgtttg
     3061 gcggcaattt tctcatcaaa aattaacaac gtcaatatta aattaaaaag ccctaataaa
     3121 attatagtat ttaacgaaga atcaagtatt ggagaaaata aatcagaatt agatgcagaa
     3181 gtagagggtg aaaatcagga gttaattttt aattggcatt attttcttga tggtttaaaa
     3241 ggtattgatg gagaaaatgt tttgttgggt tttaatgggg atattaaacc agtattgata
     3301 aaatccacca acaaagatga ttatttttat attttgatgc caattaaaaa tagttaatat
     3361 gaccagagaa gaagcttttc atttactcaa aaataaaatt aatgatccaa atatgatcaa
     3421 gcattcttta gcagtggaag cttgtttgag ggagttggcg cgttatttta atcaaaatga
     3481 agatcagtgg gggttggcgg gtttattgca tgactatgat tataaagaga tgatcaacaa
     3541 tcccgagcgc catggtcttg cggcggctga agaattagaa aaattagggt tgaccgatcc
     3601 ggtgattctg caggcaattc gtgctcataa tgaacaaaac catactcccc gagaatctct
     3661 tttagataaa gcgattcatg ccgtggatcc tttaactgga ttaattgtgg ctgccacttt
     3721 agtgctgcca tctaaaaaaa tacaagattt aactgttgaa aatattctaa aacgttatcg
     3781 ggaaaaacgt tttgctgccg gtgcgaatcg agaaattata gctcgttgtg ttgatttaaa
     3841 tctcagtcta gaacaattta tcagcatctg cctacaggca atgcaaaaaa tcagcgacaa
     3901 tttaggttta taattaatga agcatctcta atattgagag ttgttgttct attttttctt
     3961 tcaataaagg atatttattt aatgttgggt cagtatttaa taaattttct gcttcttttt
     4021 tggttatttc gatgagattt aaatttttta aagcctccat aaaataatca ggcaatcctg
     4081 attgacgttt gcctaaaaat tctccaggac cgcgcatttg taaatctaat tctgccaact
     4141 caaacgaatt tttcgctttt aatattgctt caaggcgttt gtgactattt gcattactag
     4201 tttccgtaaa aagaaaacaa tatgattgat gattacctcg accaacgcga ccccgaaatt
     4261 gatataattg tgccaaacca aaacgttctg ctccttcaat catcatcaca gtggcattag
     4321 gaacatcaat acccacttca attaccgaag tggaaattaa aatatcaatt tcttgattaa
     4381 gaaatttttt cataatacta tcttttgttt cggttttcat ctttccatgc agcatagcaa
     4441 ttgagaggtc ggggaaaact tcttttttta atttttcgta ttcttgagtt acacttttaa
     4501 cttctaattg caatgaatta ttaataagtg gacaaattac aaaaacttgg cgaccggctt
     4561 gaacttgaga acggataaat tgataaactt tttctctttg aggaggagta attattttgg
     4621 taataatcgg tttgcgattt ttgggcaatt ctttaattga agaaatatca agatcgcccc
     4681 acaatgttaa agctaatgtg cgcggaatgg gggtggctgt cattgaaagt aaatgaggga
     4741 taaaattgtt tttattttcc aaaagttttt ttctttgctc tacgccaaaa cgatgttgtt
     4801 catcaataat tgccaatccc aaatgtggaa agcgaacgtt tttttgaatt aaagcatgag
     4861 tgccaataat aatttgaatc tgttgttgag cacattgttt taaaaaatct tttttcgtca
     4921 ggatagaaac aagtccattc caactgattt gggttgttgc agaggtgatt aatccgatag
     4981 taatatcaaa atctttaaat aattgagtaa aagtacggaa atgttgttca gcgagaattt
     5041 cggtaggcgc cataaacgct acctgataat tattctttac ggtcattaaa gcggcgatgg
     5101 ctgccactat tgttttgccc gaaccaacat cgccttgcag aagccgattc attggtttgt
     5161 ctttttggag atcttttaaa atatcgagaa tggcgttttt ttgattttgg gtaagttgaa
     5221 aatttaattt atcggtaaat tttttaagcg cttccatatt ggatttaata taaaatccgc
     5281 gatattgttt taattgcaat cgctgtttgg ttacaatgag ttcgaggata aagagatttt
     5341 caaaatgaaa tctttttttg gcagcttcag catttattaa agacgttgga aaatgaattt
     5401 gatggagggc ggtttttaaa tctaagagat gatatttttg acgcaaaaaa agaggcaggg
     5461 gatctattgg aaaacgagtc tgattgagac attttttgat aaaaaatctt aaagctcttg
     5521 agctgagtcc tgctgtctcg gaataaaccg gaattaaacc agcagtttcg cgtcgattag
     5581 aaaaatcttg ttgatatatt ttttcgtaat gagaaggtat aaaataagta ccattttttt
     5641 ctttactaag acggcccgca atactaatcc aatcattagt tttaagattt ttcattaaaa
     5701 atggctggtt gaaccaaata actttcaaag tgccagtttc atcttctatc acagcttcag
     5761 tgataaacat ttttttatgc caagtgcgaa atgttttgat ctgtaaaatt tttcctacaa
     5821 tggttgcaac ttcatccggt gttagctggg aaattttttt aatttgagaa aaatcctcgt
     5881 atcgaaaagg aaaataaaat aaaagatcgc ctactgtttt aattcctaat ttatagaatc
     5941 tttttaaata ggcgggtccc acaccgggta ttaattgaat aggtgtttga aagtcaaaca
     6001 tattatcatt ataaagtcca aattggaaaa tagacagtct tatagattat agtataaaat
     6061 taaactttta gagaaaatta atttttagag attagatttt taatattaag atgagaaatt
     6121 ctttgtggat tttttaagaa agcggcggaa aatttctttg aaattttccg ccgccggttt
     6181 ttattttttg tcattatgag tacttttttc attgtcatcc tgttttttta tcgtcatcct
     6241 gaggacttta gtccgaagga ccccatggct tttagcaata atcagcaatc tttgctgtgg
     6301 atgggattct tcggacttcg tcctcagaat gacaaaaaat aaaaacactc agaataactg
     6361 ctgtttttct ctgtcatcct gagcgttagc gaaggatccc tcggctttca gcaatatagt
     6421 tttaacgtcg tttttgctaa atgtaaaata ctttataaca ttctcacatt cttgagaatg
     6481 tgagaatgtt tagggtgtgg cggcgaaggc caaaggcctt cgccgccaac agtttgccgc
     6541 ccgaatattt tctgccacca gattatttat tagctcatga attaattcat gagctgaagc
     6601 aattaattat tttttattgt acccccacga ggaattgaac ctcgatctca gcctccgcag
     6661 ggctgcgctc tatccgttga gctatgaggg ccttattatt aaaagagatt agctaataaa
     6721 tccatgagcg atggtttaat ttccttttct tttaaaaaat tattcaagaa ttctcgaatt
     6781 ttgacagttt tttctttttt aaaaggaaga aaaatgtgtt tatcaaataa tttatcagtt
     6841 tcaaaaataa taaaattttt atcagctgtt tctaaaatag aataatactt aatatcgtga
     6901 tatgaaacaa aatcatcatc aacataaaac ccatccggag tgagacgata ccacgttttc
     6961 cgagatttta aaaataatag aacaaaaaga agggcgccta aaattagaat agccaaaaag
     7021 atccaattgc tggtgataat agcaacaata ctacagataa cgattacagc aaaaaaaatg
     7081 attgaaaacc aagaattaat tgcatatttt ttttcaatag tccaccaaat aggctgcaat
     7141 tttttttgtg cactaagtct aggcgaaacc tctgacgttt tttcattaaa ggagacactg
     7201 gcgagttcat tactatttga aaagcgtgtt ttttcttgaa tattttttga cgctgctttt
     7261 cgtaaatcta aaacgtcggt attatccgat ggcttatttt tttgacgagc aatcgcatct
     7321 aattttttac tcattgtttt gatttaaaaa attttcttta aggcgcagat taatgcgttt
     7381 ttcgttcaga ttaacggtat cgattataaa ttctaactct tgattaagcg cgagcttttg
     7441 ttgcatggct tctataccac caaattcaga agaatgaata aaaccaaaaa catcatcttc
     7501 aatttgaatt aaagctccaa agggaataaa tttatggact tttcctttta tgacttgttg
     7561 gggttgatat tgctgtaatt ttttaagaac aggatcttct ttttgcgctc ttagagaaag
     7621 gataattttt ttatttttgt taatagaaat aatttgaaag cgataaactt ggtcaggctt
     7681 aatgacgtct tctaatttat caatgggcgt ccaagaaact tcttctaaag ggaggaaggc
     7741 ttttaattga ggataatctt taagattaat aatcgcacca ccgttgataa ttttgtttaa
     7801 agagccctca acctgatcgc ctacctttaa tttatctata atatcttgca tggcgtcttc
     7861 ttctattttc ttttctgaaa aaataatttt tcctaaagca ggattaaaat tgataatttt
     7921 gacctcaata tcttgaccaa ttaattcttc aagcgcttca agaatttttt ctttatcacc
     7981 tccttcaact cgaggaaaat gtttttctga catttgggag gttggcaaaa aaccttgaag
     8041 gtcattaatt ttaatgagta aacctccacg attagcgctt acgactttgc ccacaataga
     8101 ttggttatta ttcatgagat ctttaatcca tcggtattga ttgtcttgag ggttgtcttg
     8161 caaagataca tcaaccatcc caaattcatt ttcagggctg agaactttta cgcgcacaac
     8221 atcgccctct tttaattcct caatgtattt ggcagagcga gtaaattcag ctccccaaac
     8281 aattccaatt ccgtattggc ctaaatctaa ataaaggctg cgaggagttt tccttaaaat
     8341 ttttgcctca acaacactgt ttgtatttaa tcgaggaaca tcaacgtgat gccaccaagt
     8401 tggtctcttt ttaattgcgg gtgaggaaga attttgatta accatatttc aatatattat
     8461 caatttcttt taaaaagtcc agagggttaa tgagaataga agttttttaa gtaagaaagg
     8521 gagatgggcg cggcggtaat tttcttttag aaaattaccg ccgcgtcgcc gcctttcatt
     8581 ttttatttgc aacttaactt cataaaattt aagcgaattt tcatattttt ataacaaaaa
     8641 atcttccaga atttgatttt ttcatttaaa attgctaaaa ttttcgtata tgactattga
     8701 atggtttggt gaaggatgtt ttaaaattac agaaaccggc aaccattttt cgattttaac
     8761 tgagccaccg actcgcgaat caggtctagc tgtaccacgc cataaagttg atgtggcgct
     8821 taatgttttt tcagatctct cggatagttt gttcagtcag gaaaagaaag agaactttgt
     8881 cattactaat gctggtgatt atgaattaaa aggggttttt attagaggca tccttttgag
     8941 tgttaataaa agcattatta aaagcgctta taaaataaaa atggaagata tcaacattgg
     9001 ttatttgggc atgattaatt caaaagaaat aacgccagaa gtaggaaatt tttttagcga
     9061 tactgatatt gttttggtgc ccataggagg aggagacgta atagacgcgg cggaggctaa
     9121 aaacattatc aagcaattag agcccaaaat cgttattcct atgtattata aaattccagg
     9181 gttgaaaata aaaagagctg agttagaaaa ttttttaaaa aaaatggaag ttaaatcagt
     9241 agaaaaaaca gagcgattgt tgattaaatc caaagattta gaaaattggg gagaagaaat
     9301 gaaaattttt attgtcaatt ctttatgaga tcatttcaaa caattcaaaa taaattaaat
     9361 cagaaaatac aggccttgcg ttcccagccc tatcaaaaaa gagtagtaat aatgcaagtt
     9421 tgtttaatgg ttattatttg tttgttgttt ttaggttgga ttttcagcgt aaaaaacaat
     9481 gttgctgaaa atcccgtcat tcaaaaaacc caagaagcca caaaagaaga aacagataaa
     9541 cccaaccgtt ttacgatttt tcagaaagga atagagctgg tctatcaaga ctatttgcgt
     9601 ccgtttttcg gcaaggtagg ccagatttta tccggagcat tttctcagtt tattggtttg
     9661 gtaattgata ttgcccagcc gattagttat gggatgaatc aattaatcac tcaatataaa
     9721 gcttacgtgg agttagttct ttcgttagta aaataaaaaa taatgaccaa aaaatttaaa
     9781 tctaaacgtc tcaaaaaaat tcaaccatcc aaaatagacg aaaaacagaa tgaatcttct
     9841 gggttgggaa agtcagacca aattattctt agagaaatta ccgaagaatt aaaaagtgct
     9901 taccttgatt atgcaatgag tgttattatt tcgcgcgcat tgccggatgt gcgagatgga
     9961 ttaaagcctg tgcaaagacg cattctttat gcaatgtcag aagcaggttt ttggcatagt
    10021 agtaaattta gaaaatcagc aagtattgtg ggcgaggtat taggtaaata tcatcctcat
    10081 ggtgatgtgc ctgtctacga tgctttagct cggatggctc aatctttttc tttgcgctat
    10141 cctttaattg agggacaagg caattggggt tcaattgatg gggatccgcc ggcggcaatg
    10201 cgttatacag aagcgcgttt ggctgttttg gcagaggaaa tgctgaaaga tttagataaa
    10261 gaaactgttc cttttcaaga taattatgat ggaacgcgca aagaaccatt agtgttacct
    10321 gcgcgcctgc ctaatctttt aattaatggc acttcaggaa ttgctgttgg tatggcgaca
    10381 agtattccgc ctcataattt aggcgaggtt tgtgatgcgt taatttattt agtggatcat
    10441 ccgaatgcag aagtgaaaga tctgcttcaa ctaattcaag ggccagattt tcctacgggc
    10501 ggagtaattt ataatcgtca atcgcttgaa gaactttatg ccagcggtcg tggaactatt
    10561 acagtacgag caacggttga agttcaagaa agaaaagccg gtcaagccga tcttgttgtc
    10621 tcggatattc cgtatcaagt caacaaagcc gatcttttgg ctaaaattgc cgaattagtg
    10681 caagacaaac gaattgaggg cattagagat attcgtgatg aatcggataa agaaggactt
    10741 caaattacta ttcaattaaa aaacgatgcc catcctcaaa aggttttaaa taacctttat
    10801 aaacacacgg atctccaaaa gaattttcat gttaattttt tagctttggt tgaaggcatt
    10861 caaccacaga cgctttctct taaagggctg cttgaagaat ttattaaaca ccgccaggcc
    10921 attgtttata agcgttcgca atttgatctt aaaaaagcaa aagatcgctt acatattttg
    10981 gaaggattac taaaagcctt aaaaaacatt gatgcggtta tcaaaacgat taaaacttct
    11041 aaaaacagag acgaagccaa agaaaaatta attaaacaat ttaaattaac cgttattcaa
    11101 gccgaagcaa tattggaaat gaagctgcaa acattagtag ggctggaaag acaaaaaatt
    11161 gaggaagagg ccaaattaaa agaaaaagaa attaaagagt tggaagaaat tttaaaaaat
    11221 cccaaaaaga tccttcagat tattaaagac gaaacattag aaattaaaaa gaaatatgcc
    11281 gatgaccgta aaaccagagt attaaatatt ccaataggcg aatttaaaga agaggatcta
    11341 atacccgatc aggaagtagt gattatgatg agtcaagatg gctacattaa agcttttgag
    11401 ccagaaatta ttaaaaatca aaaaagagga ggcaaaggga tgataggatt tgacgtcaaa
    11461 gaagaagata gaattcagca cgtattattg gccaacactc acgacaattt actttttgct
    11521 actagtagcg gaagagtttt tcaattgcga gcttttgaaa ttccgatggc tactcgaaca
    11581 gcgcgaggca gaagcatttt taatttcctc gatttatcca aagaagataa aatagctgcc
    11641 attatggttt atcccaatca gcaatcaaaa aaaagcggat atctcgtaat ggtgaccaaa
    11701 aatggaataa tgaaaaaaat gaatttgact gaatttgcca acgtgaggcg ttcagggttg
    11761 attgctatga aattaaaaga aggtgatgag ttgaaagatg ccaaaattgt tgagcccaat
    11821 gatgaaatca tagttctttc tgcgtatggt caagccctgc gatttcaaga aaaagatttg
    11881 aggccaatgg gaagaagcgc agccggggtt ttaggcatcc gtttgaaaac agagaaggat
    11941 aacgaagtga ggggctttga tgtgatcaaa ccagaattaa aagacggaat gttgctggtg
    12001 gtaatggaaa aaggatttgg caaacgcact gctttaaaag aataccgatt acaacatcgt
    12061 ggagggcaag gaattaaaac tgctaaaatt agcgaaaaaa caggaaaagt ggtagcgatt
    12121 aaagtgattg ctaaagatac taaacaagtt ttattaattt cgaaaaaggg gattttgatt
    12181 aaaacggatt taggtaatat ttctcagcaa tcgcgcattg ctcagggggt gagaattatt
    12241 cggcttgatc cagatgatgt ggtggcgggc attgtagttt tgtagttttt tttgatttgt
    12301 gataaaataa atttatatgg cccacatctt tgaccctaaa aatttagtcg ctcaattaaa
    12361 tctggcgcca ggctctgttg tggcggattt tggtagtggt agcggaattt tgacattaga
    12421 attagctaag gctgtaggcc cagaaggaaa ggtttttgct gttgatgtag tgtctcaaca
    12481 gttggaatca gtgaaatctt tagcacaaat gcgtggtatt aataatattg aaacacgctg
    12541 ggctaattta gaaaaaacga gcacactaga tccaaatagt tgtgattggg taataatagc
    12601 caatttatta tttcaagttg aaaaaaaatt tcagccacgt attttcgaag aagcatataa
    12661 aatattaaaa accgaaggga aactggttat tattgactgg aagccagaaa gcatattagg
    12721 cccaccaaaa gagaatcgtc taacaataga agaagttaaa gaaatggctc aaaaaaagca
    12781 atttaaattt atgcatacta ttgaaattga cgacacgcat tggtgtgcta tttttcaaaa
    12841 ataaaatatg cctcaacaag agctaaccaa atctcaaaaa attattttta tagtgatcgc
    12901 tattattatt ttaatggtgg tattaatgtt actcggcgtt attcctggat taaaatctaa
    12961 agagaaagaa aaaacttcgg aaatgccagc cattacatta gaattttggg gtattgataa
    13021 tccggacgcc ttcaagggcg ttttagaagg atataaggct gtttacccta acgtcacaat
    13081 taattatcag caaattgatg aagttggtta cgaaagcaaa ctgctgaatg ctttagctgc
    13141 tcaaaaaggt ccggatgttc ttatgattaa acacaattgg gttcctaaac atttagataa
    13201 attatatccg tcaggcgttt tgctaaaaga tttgaaagcc ggttttgttg atgttgtagc
    13261 ggacgatctt tattatcaag gaaaagtttg ggctttacct ttatatgtgg atactttggc
    13321 tctttattac aataaagatt ttttcaatac agctggcatt gtaatgccgc cagtgacttg
    13381 ggatgaattt gtaaaagatg cccagctgct tactaaaaaa aatagcagtg gcgaaattct
    13441 gcaagccggt gcggcactag gtacggttaa taatatcaaa aatgcagttg atatcgtttt
    13501 acttttaatg ctacataata atcaaaaaat tgttgatgaa aatggcagag ggatttttga
    13561 tactgcgaat ggtgtcaatg ctgttaattt ttatttaagt tttgctaatc cagcaagtcc
    13621 taattattca tggagtacac attttcctaa tgcgaccgaa gtttttgctc aaaataggtt
    13681 ggcaatgatg attgattata gttcagcaat ttcaacactc aatgccttga acgcctatct
    13741 taattatggc atagcgccct tacctcagcc tgcaaatact attaccaaac aaaattacgc
    13801 tgattactgg gcttttacag tttcagctac ctcaaaacac cctcaacaag cttgggattt
    13861 tatttattat cttgtggaca agggagcggt agaatattta aatagcacct tgcggccagc
    13921 agctaatcga gtgttgattc aagcgtgcaa aaatgatgaa aaattggggg ttttttgtga
    13981 gcaagccctt acagcaaaaa cgtggttgca atttaatccg gaagagaata caaatatttt
    14041 aaaaaatatg ttagaatcta ttttagatgg tcgagccacg ccgctcacag ctttgcgcga
    14101 agctgctcag cggattaatc gtagtgctaa tcaattttaa tgaaattttt ttctagatac
    14161 tcttttgtag gagcaataat ggaaggcatt atattgttaa tggctagttt ttatttaagc
    14221 aattatgctt tagctcagca agtaactcaa gttgttcctt cgccagtacc tgcaccggta
    14281 acggtgactg tagaaaaaac aggtgacggg ttgcttagcg gggtagcaac gacatgtaga
    14341 gaagctggtc aatgtacttt tggtcatatt cttattgtga taacaaacat ttttcatttg
    14401 ttaagacaga ttgcttttta tgcagctata ggatttggtc tttatggtgg tatccggatg
    14461 attgtttctc aaggtaagcc cgaaggtgtt aaggcaggcc agaagattat tgaggcggct
    14521 tttattggtt tgattattgc ttatggcgcg agttttatta ttaatattgt ttgtaccatt
    14581 cttcttggtt acccgctaac tttaggtaat attcttaatg ctaattggat tagataattc
    14641 tatgtttttt tcctattctc aaaagaaaaa aaataacttg acgaaactat ttttattttc
    14701 tacactatta gtagcaataa ttttgataac atcagacgca tcagctcaat tagtcagtcc
    14761 tatcaaggct aaaactatag gagggttgat gcagcaaata gtccaatgga ttcttaatct
    14821 agccgtgccg cttagcgctg taatgactat ttggggggca gttttattga tgacagccgg
    14881 tggaaatcct caaaaagtta aacaagcaca gaatgctttg ggatgggcta tgggtggttt
    14941 ggttgtagta atgttgattg aaggattatt cggttttggt aaagatttaa ttgctaatgc
    15001 ctcctcttta actcaattga ttaacacgat tcagcaatac cttttgttga ttgtcggtcc
    15061 tttgtctttg gtaatgtatt tctacagcgc ctttttaatg agcacgagca atatagaaag
    15121 cattaaaaaa gccaaatcaa ttttaatttg gacaaccgtt gctgttgcta ttattagtat
    15181 tttttcggtc gctaatctta ttaatttaat taatcaactt gcaaaataat atgataatca
    15241 aaaaattttt accgggtatt ataatgatta ttgttgggct atctttaata tcgccggttt
    15301 tggctcaaca aaatcaacta gcgacaagca gtattcgcat tagcggcgtg caagacatca
    15361 ttaaaattat caacaacatt gccaagtgga tgtataccat tcttttggcg ctagtggtga
    15421 tttttgttct cgtagcagct tttacttttc ttacagctgg tggcaaaaca gagaatgtga
    15481 aaaaagctcg taatcaaatt atgtatgctg ttattgcact agtggtggcg ttattggcgt
    15541 ttagcattaa cacaattatt accaatattt tattagcgaa ataataggtt attataatat
    15601 aaaggtctat aataaaaaat taaaataatt aaatgaaaca aattattaca atttttctct
    15661 taatagcatc aatgagtttt actataggtg gcttggtgag tgctcaggtg tatacaaatc
    15721 tgcaggagcc tcctgctcct cgtgatttac acacttggat ggggacggtt aacacaattg
    15781 ctcaatggat ttacacgatt gttttagtag cagctgttat tttgggttta atagccgctt
    15841 tctcttatat tacggctgct ggtgatagca ataaagttaa aactgctaat aacatgctta
    15901 tttatgctgc gattgctgta gttattgcta ttcttgcctt tagtattacc aaaattgttg
    15961 gaggtttgtt aggcactcca atataggatt aattaaaaaa tattaaatta ttcctaaagg
    16021 tcgataaaat aaattataat gaaaaaattt tttgctattg tttcgttatt agcggtcgtt
    16081 tctttaacgt ttggaagtgt agttagcgcg caggtgtata cagggcctgc taatttgcct
    16141 acgcctcaga atttacccac ctggatggag acggttaaca caattgctcg atggatttac
    16201 acgattgttt tagtagcagc tgttattttg ggtttaatag ccgctttctc ttatattacg
    16261 gctgctggtg atagcaataa agttaaaact gctaataaca tgcttattta tgctgcgatt
    16321 gctgtagtta ttgctattct tgcctttagt attaccaaaa ttgttggagg tttgttaggc
    16381 gctccaatat agaattaatg gcgatatttt ctaaatttaa aactagcctt taggctagtt
    16441 ttaaattata ttaaaatttg cgttaaaaag agattggcta tgattaagtt taatatattt
    16501 tttggttttt ggttagaaag acgtgtttag ccccgctttt ttgaatgaag agattgcaga
    16561 aattttctaa aagaaaattt ctgcagtatc tcagtattta ttttataccc tttatttttt
    16621 aacttcgttt tagtgctctt ttactcaaac tcatttcatt cgtttgtgtt cagggactcg
    16681 gctacaagtc gctcgagctt ccgctctcgc gctcgcagcc cgccgctcgc tgggctcctc
    16741 gccaactcgc tcgtcgccca tagctgcgcg cttctcgtcc ccacgcaagc caagcagttg
    16801 gcttgctcct cggctcaaac tcatttcatt cgtttgtgtt cagggactcg gctacaagtc
    16861 gctcgagctt ccgctctcgc gctcgcagcc cgccgctcgc tgggctcctc gccaactcgc
    16921 tcgtcgccca tagctgcgcg cttctcgtcc ccacgcaagc caagcagttg gcttgctcct
    16981 cggctcaaac tcatttcatt cgtttgtgcc gagggaggga ctcgaaccct caacccccaa
    17041 aggggactag gtcctaagcc tagcgcgtct gccaattccg ccacctcggc aaattttatt
    17101 gtacaaaaga cgatcaataa tttagaaaaa atttttattt ttactttata ttaactctga
    17161 tttaagttat agcgtttcaa cattttgtgc ataaggaaaa tctaagcgca aaacatactc
    17221 tatcttacca caataagatt caacagctct tcaaaggctt gtttgctggt tgctacgaga
    17281 aaataatact cattaccata gaaagcataa taaaattccg caccagttga aaattttagt
    17341 accgaagcat tcaaagggcc gattgtttgt ggaagaaaag gagagttagg tttgccggga
    17401 ttttgcaaat attgataagt taacagctct gtattattga gaatatagtt ttcaaatgct
    17461 ctagaaaaat cctgttttaa ttttggcagt acaagcgggt tagattttgc agtatcaatt
    17521 tttgtgacat aagcgattga ttttgaacca ctgttgtcag taaagacaat aaaagaaaat
    17581 ctattttccc attgtggccg ttcggcagct gtaatgccct taaaatggtc aaaaagaaca
    17641 tcaaaaaatt cacttgctgt tacaagcgag ccgttgtttt ttacaaaaac caattctttg
    17701 gttttttgag gctcggaaat ggtatagaat tgctttacta aaatatcttc aaatccattc
    17761 aaagttaaat caaacgaaac ccattcctga acaccgttaa aattacgcaa aagagatgaa
    17821 tattgaggtg tgagagaggg cgtggcaata ggcggtgatg tttgaggttt gtgactaata
    17881 tagaatacta atccaactat aatgcctatt ccaacaatgg ttgcgataat gatgatgggt
    17941 actttatttt ttttctgagc gatattagaa gtatccgttt gggtgggaaa gaatgttact
    18001 gcgttcttgt taccttcatt gtgaatactt cgatttattt cttctggggt gcggccgaga
    18061 actttgccgc tgataaaacc gccaccgctt ttgcgcatat cttccacatc ggtttctaaa
    18121 gtgcgaattt taatttgaga aggcggtttt cttaaaattt ccggcataca tttcaattat
    18181 atctattaaa aattaaaaat aaaggcaatt tcttttagaa caacgattta agcaattgtt
    18241 cttaagactt ctaatgctgc gccgagaatt aataaagcca ctaatataaa tctcaaaaca
    18301 tttggaattt ttaagacaaa aggaggctga gtagcaccgt tggtttgggc ttttttatga
    18361 atgagaaaag taattaaact ttcaattcct aaaactaccg ctcccaaaaa accgatcact
    18421 ttaataaaat catgggcgcc taattggtaa agaatgaggg gtaaaaaaat agcgatcagc
    18481 caggcaaccc attgtttcat atgcaaatca aaaaagagag aatttttaaa atttaaagat
    18541 aaaataaaat atgaagtaag aattaataaa atgcccaata aatcagtcgc aatgcccaaa
    18601 agaggattaa tgctaataag ttgaggcaca agatcaacgc caatgttttg gactcccaac
    18661 aaacctatgg cgccgagaca aaatataaga taaagcaaag cagggataac gctgccccag
    18721 acgatgggtt ttttaaaatt aatatctttg ggagtaagtg attttttttg aaaataggaa
    18781 taaatttcag gcacagccga ggctcctgat agggcaaaga gtaatactcc ataaggcagg
    18841 agtaattgag aagcattgat taacggcaca tgtgctaaat ttaattgagg aatgcctaga
    18901 ataaaaaaaa ccgcaatgag cattacaatt aaaattaaac taattgtttc tcctttgcca
    18961 ataaatttta ttcccggcag tgcgcccaat gaaccaataa cccaaaacag taaaacggat
    19021 tgagtttgat ttaaaggata atgtataatc tgagaaaaat tttggagaaa ttggccacct
    19081 aaaattaaat aagcaagaag acatccgcca atactgataa aactggtaat agtaattaat
    19141 gtttttgccc aatgtcccag atagatttca gccataccaa taaaacgatg ttcgccttca
    19201 gtgcggagca gagattcgcc ataagccaga tgagtgacgg tgcaaattgc agtgactaaa
    19261 attaaaaaaa taacaaataa aaaagaccct gcatttataa aaacaaaggg caaactaaac
    19321 ataccggcac ctaaaatggt gccagctaaa atagtagaag ccgcaaaata attagcaagt
    19381 tttttagata aactcatctt ttttagccac ctttttttct agacggtttt caataaagaa
    19441 ataaaaaaca attaaaacaa ttaagacaag gcaggttgaa agaatggcga gagtgtaaag
    19501 tttaaatcct acagccatgc caatagcagc tgtgacccac aaactggcgg ctgttgtaat
    19561 gcctcgaatt tttgattttt gataaataat tacgcccgcg cctataaaac caatgccgac
    19621 aataatttga gaggcgatgc gagaaggatc aaaagaaaaa tttggtatat tggcgtatcg
    19681 caatgccaat gtttcagaaa taatagtgaa aacagtcgca cttaaagaaa ccaaagaata
    19741 agttcgaaaa ccagctggtt ttcgagccaa ttgtctttct aagccaatta acgaccccaa
    19801 aaataaggag agtagtaatt ggcttaacat aataagatca ttcattattg aaggaagttt
    19861 ttattttatt cgaccaaata aacttcgttg ccgtcgtgta ctttttgatc cactttaatt
    19921 cccacctcat cgcctttttt tgctttttga acggattgat gctctatttg aatagaagta
    19981 atatcttgtt caaaaagatc acccaaatta ctgcggaaaa gaacgcgatc acccactgaa
    20041 agaggtgctt ttaattttaa ggcggctact ccgattttat cgaaataatg agtaacgatg
    20101 ccaatcaatt tttctttctt ttctttggtt tgagagtttg cttttttagg cataatgttt
    20161 ttttaaaatc gaccttttat ataatgtact tatatataat gtacttattg attatttgtt
    20221 ttttcttcca aaatattttc gattagttct tcaattaaag cttttggatc actgttaaaa
    20281 acaactgagc cgggaccgcg aaatgcttta tcaataattt gacgaatcat atcggctatt
    20341 ccacctgtgc cttccaaaac tcctgttaat ttattttctt caaaagctac ggtaaattca
    20401 tttagtgttc ctatacgtcc gcaaatagta ataacagcat ctgccgaacg agtgagtagg
    20461 agatttcggc cagcataccc cgcgccagta taaatgatta aatcatgata atcgatagga
    20521 aggtggtatt tattaatatg ttctttttca gaaatggccg gcgacaaacc aataaccatt
    20581 cctccagctt gttttgctgc tttggcgacc caataaggtg cacctgttgt agcaccagtg
    20641 acgacaatac agtcatgttt agccaattct tcacccagct tcatcgaaag ctccatagcg
    20701 ttagcgctac aatggcctgt ttcagcagcg cctgagatgg cgattttaat tttagattta
    20761 aaaatttcca tatagacaat tatatcaaaa aaaatttaaa attcaaaaag caaaagctat
    20821 tatggaccac ttaccttttt tatttgatta cttcttttta aattaaatac ggcggggaaa
    20881 attaatgata tagaataaaa gagcgattac tcgctctttc tgttttggag tcacattaaa
    20941 gctcaattaa ttttttaatt gttggatcat taaaattaat aatccaagcc gctccttctt
    21001 tgctaaaacc atagcggaca gcgttagaaa gaccaagcac tctgcagctg cttttatctc
    21061 tgacataaac aatgatattt ttttgatcaa gatatctgcc cttgatattt ttgcagcggc
    21121 aactttttaa acgttttgtc agtggcacaa ccgaatggca taaatcacaa gctagaagtt
    21181 tcatttttac ctcccattga aaattattaa tgtgctatca ttatttttcc ttccgatttt
    21241 tgatgttgaa ttttttaatt actatgatgt ttccgtgcgt ttattttttg tagtccgccc
    21301 agcaggactc gaacctgcaa ccgtttgctt aagaggcaac tgctctaccg attgagctat
    21361 gggcggattt atatttagat ccaaagtttt aaaataaaaa tgggggattt ataaaattaa
    21421 acaggcaaat tgtagtttaa actcttttta tcttacctaa aaattttatt ttcttcaaga
    21481 aaaaacagac ccattaaatt agggcctgtt gatatttttt tgagcaacct ctgcaatttt
    21541 atccataatt ttattagtgg cttggataag aatttgagga ttggcgtcga tttcgcgttg
    21601 tgattgagat tgaaactgga atggtttgcc gatcacaaga tttttactcg cgaaaaaacc
    21661 gagtagccct tctttaaatc ccgaagttct gggaccaaaa gagccaatag ggataataca
    21721 acatccgcta agaagagcaa taactgcagc gcctcttctg cctcgtttta atactttttt
    21781 gggattgcga ccgccttcgg gaaaaataac tacagcgttg cctttttgga ttaactccgc
    21841 tgagcgttta aggctgctgc ggtcagccgg cagatagccg atactatcca ttccgagagt
    21901 gagtagggca ctaatgaaag ggtgacgaaa aaattcattt ttgagacgag gtgaaccgat
    21961 aaaatagatc ttgtgatttt ttggtttgag atggcgttgg aaaaaaggat aaagagccac
    22021 ggcaataatt aaggggtcat aattattttg atgattggcc acaataagaa atccgcgatc
    22081 tttaggcaca ttttctaaac ctgttacttt tttgagctga ggtcgcatca acgaataaat
    22141 caaccaataa aatgtcttat agaaaaacat tctgcctcct tatataaaat tttgaaataa
    22201 ccgaatttac cttaccacat ttcttatttt ttgcaagacc gcgcataatt agaacacatt
    22261 agaaagttgt catttcggtc acttttttgt tgtcattctg gacacagctc cgtctaaccg
    22321 gcaggtagga gagaatccca cccacagcag taattatcga ttattgctga aagccgtggg
    22381 atccctcgga cttcgtcctc gggatgacaa ggggaaaaca ggataacaat aaaaaatttt
    22441 tcaggatgat gatttcgaat ttttgatcgt gcccccggga ggattcgaac ccccaattcc
    22501 agttccgaag actggcgtga tatccatttc accacgaggg cgcgataata atttatcatt
    22561 ttttctttga aaattcaaat ttttaaagaa ttgacagagt aaagtttttt ggctaaaatt
    22621 aaaaagatga aagatgatca gaaaaacttt tggcttaaaa atcggattaa tttagcttcg
    22681 ccccaattag aagcgtctcg ggaaatgaca aaaccaatgg aaattcctat tgaaattaaa
    22741 aatattttag agcaatttaa taaaacccct caaaagttcg aggcttatct tgtaggcggt
    22801 tgtgtgcgtg atgttttatt aaagaaaaaa cctaaagatt gggatattac tacgaatgcc
    22861 actccgcagg agattcagaa gttatttccc aatagctttt atgcaaatga ttttgggact
    22921 gtttcggtta ttactaattc cgacgatgaa accttgcaag ttgtagaaat aacaccattt
    22981 cgattagaag gaaaatattc agataagcgt catcctgatg aaattaaatt cgccacaatt
    23041 ttagaagatg atcttgcgcg tcgagatttt actattaatg ccatggcgat tcaagtcaaa
    23101 gatgggaaat tgaaaattat tgatttattt aatggtcaga aagatttaaa aaacaaactt
    23161 attcgagcgg tgggagaccc agaagaaaga tttaatgaag atgctttaag attgatgaga
    23221 gcggttcgct tcgctgcgga attagatttc cagattgaag aaaaaaccaa aaaagctatt
    23281 gaaaaattac atagtaattt ggaatgcatt tccaaagagc gggtcagaga tgaattgata
    23341 aaaatgtttc attgcgaaaa tgctgctcgc ggtatagaaa tgttgcatga gttaaaactt
    23401 atgccttacg taattcccga actagaagag ggggttaatg ttgtcggagt gactaaaaaa
    23461 ttaaaagagc atattaaata cgaaagcctt actacttttg agcatcttgt taaaacttta
    23521 gattatgctg ccaaaaacaa ttttaattta gaaacgcgat tggctgcttt gttgcatgac
    23581 gttggcaagc cacttacaaa aaaaggtgaa ggattagaag caagttttca aggtcacgaa
    23641 cgggtaagcg ctcaaatgac aaaagaaatt ttaactcgtt tacgcttccc taaaaagaca
    23701 attgataaag ttgttaaatt agttcgctgg cacttcttct tttatgatat taataaagtg
    23761 acaccagcag gtgtgagacg tttgattaga aatgttggtc ctgaaaatat tgatgattta
    23821 ttaaaattgc gagaagctga ccgtagtggt tcgggattac caaaaatttc ttcctatcga
    23881 ttgcgccatt taagatatat gattgaaaaa gtaaaaaaag atcccataga accaaaaatg
    23941 ttaaaaatta aaggtgatga tgtgatgaaa attttgaaca ttcctccttc tccaaaagtc
    24001 ggatgggtat taaacatttt attggaagaa gtattagatg atccagcaaa aaacaataga
    24061 gaatatttag aaaaacgatt gaaagaatta ggtaaattag ccgatgagga tttaaaaaag
    24121 atggctgaag aagcgaaatc taaaaaagaa gaaatagaag aagaaattga cgaggaaatg
    24181 agaaaaaaat acaatgtcta aataaaacaa aacggggtgt agtataacgg tagtacgctg
    24241 ggttcgggac tcagtggccc gggttcaact cccggcaccc cgactaaata tttggtcgca
    24301 attactgaac aaagcgcgac catttttgac taggataaga acgaatcgtt ttttacttct
    24361 gccgcgccaa aggcgcggca gttgagataa attttaaaat gatttaaact taaaaaagtc
    24421 ggaggaatta aaattaaata gttatgaaat ttaaaacatt atttctcgta tttttaattt
    24481 tgcttattgg cgtgctggcg atatattttt ccccttttca aaaaaccaat aagacaaacg
    24541 attataattt agaagaagaa aaggccaatc taaatgaggg aaacggagaa atgactatcc
    24601 aagaaactcc ttctatgtcg ccggaagaaa aaacaaccac cctttttgat tttgatccgc
    24661 atcttttgag ctgggaagaa attaaaccaa ctgctatttg ggaaagaaga gatgctcaag
    24721 ctggcgttgt ttttcaagat aaaatttgga ttttaggagg gctgggagga acaaacacta
    24781 agactactga ttcgtactcc tcatttcctc attataaaga tgtgtgggtg actgaagatg
    24841 gagaaaattg ggaattagtt accaatgatg cgccgtgggg aaaaagaagg ggacacgcag
    24901 ccgtggtttt tcaagatcag atttggattt tgggtggggt aaccaatgcg gaaattttta
    24961 aaaatgatgt gtggagatca gcagatggaa aaaattgggt tcaagtcact gctcaagctc
    25021 cctggctgcc cagaaaaggt cacgcggcag ttgtttttaa agataaatta tgggtattag
    25081 gcggagtaag tataaaaggt gtaaaaaatg atgtgtgggt gactgaagat ggagaaaatt
    25141 gggaattagt taccaatgat gcgccgtggg gagcaagata tgacttggcc gcagaagttt
    25201 ttaacgataa gctttttata actggcggag ttgtccctgg agaatggaaa ggaatgaaag
    25261 atgtttggtt ttccaatgat ggaataaatt gggaattagc caataaagaa agcccttggc
    25321 ttggcagaca tggtttatgt ttgacttctt ataataatta tctttgggtt attggcgggt
    25381 ggtcgggcta tgggaagggt tataatgatg tttggttttc gcaagatggg aagatctggg
    25441 aacaaacatt agaagatagt ttatggccag gcagagaaga tttaacttgt gtggtttttc
    25501 aagaccatat ttggttaatg ggagggatga caactaatgg cacgagaaaa aatgatattt
    25561 ggcgttccac catttccggc actcaagaga aaatgtaaaa ataaaaaaga gagcaaaaca
    25621 aaaagcattg cccatctcct cgctttgagt aaataattta atttgatata ataatattaa
    25681 ctacgcgctt tttgccgttg agctgctaaa aagtcgtttc aaaaaaaaca aacaagttta
    25741 attagaaatt aatattatat atttcatggt tattcatcag tattcaaaaa gctttacttt
    25801 aattgaatta cttattactt tggggatttt aggagtattg atggcagtag ttgtaattac
    25861 tattaatcct gccgaatttt taaaacaatc cagagataca aaaagaatgt cagatatatc
    25921 taatctgcaa accgccatca atctttattt atttgagggt agttcacttg gcactccggg
    25981 agtaacatat atttctattc ctgctgcaaa ctccgattgc tctgatttaa acctaccagc
    26041 tggctcttgg cattgtgcca cttctgacaa ctatctaaaa gttgatggca ctggctggtt
    26101 gcctctaaac ttcagcgctt taaccatcag ttctccctta agtgttttac ctgttgatcc
    26161 tcaaaatagc gtttccgaat cctattatac ctattctact gatggctctg gttttgtgat
    26221 tacggctgtt cctgaatcaa ataaatatcg taataactca gatcttttaa ctggttttgt
    26281 cgcttccggt gcttctgata aattagtcgg tggtaatttt cccaatggtt gggtatttgt
    26341 gcctggtgat ccaaacttta aaacccctgg tttttgggtt atgaaatacg aagccaaatg
    26401 tgtttctgga aatacgcctc ttacttcacc tgatactggt taccacacct acaacaatag
    26461 cagccaacct tgcgccggcg atagatatat tgcttctaca tctgaaggct atcctattac
    26521 taatatctct cacaatacag ccaaagccta ttgtcagtca attggcgccc atcttttaac
    26581 taatgatgaa tatatgacta ttgtgagaaa tgctgaacaa gtaccagtga attggtcaaa
    26641 taatcaagtg ggcaatggcg ttcttcctcg gggtaattct aattccgcgg ctgctcaaga
    26701 tggctcaagc atttatggca ctggctatac tgatcacact catcttcgca ctctcacgct
    26761 ttctaatggt tcggttattt gggatatagc tggtaatgtt tacgaccacg ttcaaagaag
    26821 caatgacaat gcgggtgatc ttactaacac tatggttttg cccgcgtgtt ctgatgctca
    26881 agctgcttgg ggttggtgta aatatgcttc tacagaatcg ccttatgttt cttcttggac
    26941 agatgacgtt gctaaagata aggttgctcc ttctaatact tcttggacct ccagccaaaa
    27001 tattggtcaa gtttatactt acaagaatgg aacaaatcaa ggaactactg tttttctccg
    27061 cggcggcctt tggtccgatg gggcgtttgt tgggccgttt gcgttgtcct tgagttgggg
    27121 cactgacggc acgtacaaca atgttgggtt ccgttgcgcc cggtaggccg tagcaatcta
    27181 aaatctgaaa tccaaaaaat atgctagaag atctaatagt ttttcaaaaa ttatacgatt
    27241 taattttatg gatttatccc attattaata aatttcctca aaaacagaga tttgtgcttg
    27301 gtcaacaaat ccaaaacaaa ttattggaaa ttttgaaaaa tattattgaa ggcaatcaag
    27361 caattcaaaa aactgttatt ttaaaacacg ccagtgttaa tttggatgaa ctccgcattt
    27421 taattcgttt gagcaaggac ttgcactttt taagtattaa tcagtatcaa atagctgctg
    27481 aaaaaataaa tgaaataggg cgatttttaa atgcttggtt aagaagattt tcgtaataaa
    27541 attttctaac cgagagctaa atttatttat gaattaaatt tagttgtcgg ctagagatta
    27601 ttttaaccga caagggatag gatcgtgcaa cggacctttc tccgcggcgg caattggaac
    27661 aatggggcga atgatgggcc gtttgcgttg aacttgaatt ggggcactga caacacgaac
    27721 aacaatgttg ggttccgttg cgcccggtat acttccatgc ttaaattaac ctggcccgaa
    27781 caaaatcttt aaggagattc gtctgtgcca tgaagcctac ggattctatt ccttctccgg
    27841 tactctatga aatgatattt ttagggtaga gcctatggtg atttgttttg agaaaccttg
    27901 tttgtttcat gaggaaaggg aaaatacaaa ctcgtttccc cgcgttattg cgttgtcgta
    27961 acgctctgac gcggggttag agagtgtttt tttatgaata agaatctttt tgaacaagtt
    28021 gccgatttta aaaatcttca ctctgcttat tttgctgctc gtcgatgtaa acgttatcgt
    28081 agttgtgttc tcaagtttga atataatttg gaagaaaatt tattaactct aaaaaaagaa
    28141 ttattgaata aaacttatca tcacggaggt tatcacgagt ttattatcac agattccaaa
    28201 aaacgaatta ttaaagcacc tctatttcgc gatcgaattg ttcatcacgc cttatgtaat
    28261 attattgagc cgattcttga tagaggattt atctacgatt cttatgcttg tcgaaaaggc
    28321 aagggaactc atgttgccgt aaaacgatta gaaagttttt tgaagagttg cagcgcgaca
    28381 caaaaaagga gggatgtaat ttcgtcgcgc gtttactgtc tcaaatgcga tatctctaaa
    28441 tattttgaaa atgtagatca cgaaatactt ttagaattat tagaaaaaaa gattcaaaac
    28501 ggagatattt tatggttgct ccgcgagatt attggcagca atgcgcaagg cattcctatt
    28561 ggtaatctca ctagccaact ttttgcgaat gtttacttga atgaacttga tcattttata
    28621 aaacgggttt tgggcgaacg atactatatt cgctacatgg atgacttttt aatattagac
    28681 acaaataaat ggcatctccg cgaaaccaaa gaacgtctgc ggatgtttct gtgcgatcgg
    28741 ctgaaacttg aactgcatcc gaaaaaagcg gaaatttttc ctgctgataa gggaattgat
    28801 tttcttggtt acgtcgtgcg gaacaataag cgattcttgc ggaaatccac ggttaagcgt
    28861 tttctaaaaa agaaacgtcg ttattataaa acgatgataa aaaatggtca gcagacggag
    28921 gtgtttttac aaaatgtttt taattcgtgg cggggatata tatgttttgc cgattcatat
    28981 aaattaataa aagacattag agcctctttc tgccagccat tttaattaaa tgcatagaaa
    29041 atattgtttg acagcaaaat agaataaaat gtagaaaata atttgccctc atttgtcacc
    29101 ttattttttc ggtgtcatcc tgtttttctc tgtcatcctg aactttagtg aaggatccct
    29161 cggcttttag tactaatcag caatttctgc attgcgtggg attcttcgcc tgcctaaccg
    29221 gcaggcaggc tccgctcaga atgacacact accacatcgt catcctgagg actttagtcc
    29281 gaaggatccc tcggctttta gctctctttt gtcatcctga actttagtga aggattcctt
    29341 ggcttttagt actccatggg attcttccct gcccgccgaa caggcggggc tccgctcaga
    29401 atgacaataa aaatagcgtt cagaatgaca aaaaataaaa gctctcagaa tgacaaaata
    29461 aattgacaaa aaataatttt ttgctatttt taaaatagat tgagatttaa aaattaaatt
    29521 agaaaggaga agaaaaatgg atttaaaaga ttacgaagca atattaagga gatgttggtc
    29581 agcagaaaca agtttggatt cggaaaattg gtcaccacat aatcctgcgt ggggacaatg
    29641 tgcagtgaca gcagctgtca tccaggattt cttcggcgga gatttaatga ggggaatagt
    29701 agaaataaat agtggcatac aacctgtcac cacttctcat tattggaatc gtttagccaa
    29761 tggtcaagaa gttgatctta ctcgggagca atttcctttt agtgccagaa tttcggttgg
    29821 tgaaattcgt tcgcgccgct atattttttc taacccgcat acattatccc gctatagtat
    29881 cttgagatta aaattcgaaa atgaactaag acctaatccg ctgttttttg acgaaatcta
    29941 tcgattatgc ttcggggccg ctcaattatc tgattgtagt tatatgcagt ttgggtgcgt
    30001 agtttttcaa gaaggtaaaa aacagcccaa agttatgact agcaatcgtt ttgttgaacc
    30061 catgagattt atttgttcgt cggaatgcat aagaaaatat attaaatcag aacaaaattt
    30121 gatgattggt gcttgcggtc atgccgaaga gtgggcgttg aaagaattac gagaaaaaaa
    30181 gattcatcct gatagatgtt cattttacgt ggcaggggtt aagcctttta ttcatacgcc
    30241 attattaaga gaaaaagaag gattttcttg tttgcgttgt gctgttcaac tctatatgca
    30301 cggagtaaaa aaaatttaca ttcctgttca agatcactgg gcttctcaaa cgccagaaga
    30361 agctcttatt tcatccttag attttgctat tgggagaagg cagatataac aagatataaa
    30421 ttgcaaaata atgtcaaaaa cctcgatttt taatcgaggt tttttatttg ttaataagaa
    30481 aaatttctga taaaataaat tttgccgggt ccaaaaaatt taaaacgcga aatgataatt
    30541 ttatggccaa aattataaat ggctttttct ctaaaaacgt tcaaagagct gcagcactaa
    30601 ttcaacaagg cgaagcagtg gcatttccta ctgagactgt ttatggtttg ggtgcagatg
    30661 tttacaataa aaaagccgta gccaaaattt ttgaaattaa agaacggcca tattttgatc
    30721 ccattattgt tcacgtagcc aatctaaaac aattggaaga ggtagctcag attaaagatg
    30781 aaagagttta tgatttagtt aaacattttt ggccaggccc gctaacgctt gttttgccta
    30841 aacagaaaaa aattccttat cttgtaactg ctgggttaga tactgttggc gtgcgcatgc
    30901 cagcaaatgt ggttgctctt tctttaattc aagccttggg acatcccatt gctgcgccga
    30961 gtgccaattt atttactcgc ttgagcccca cgacggcgga gcatgtcttt aagcaattag
    31021 gacctaaaat taaaattatt ttagatggag gtaaaactat ttgcggagta gagtcaacca
    31081 ttttattaat taacaaaaac atccagcccg tttgtctcag aatgggcggt ttgccaattg
    31141 aagaaattga gaaagttgtt gggaaaatta caattccgaa aaaacaatcc agaaaaatat
    31201 tagcaccggg caatttgaat tttcattatg cacccagaaa atctttaatt attgttggaa
    31261 aagaagaaga aatttggcga tatcccgaga gtttgaagaa aaaaatagct ctccttgctt
    31321 ttcaaaaaat taaaaataaa agaaaatttt atgagtggcg tattttatct cctcggggtg
    31381 atttgaaaga ggcagctgct aatttttttg actttcttca tcaattagat gaaggatcag
    31441 cggagattat ttttgttgaa aaagtaaaag aagtgggttt gggtcgagct attatggaac
    31501 gtttaaataa agcaactcat caataatgaa taaaaatata attatcctta ttgccattat
    31561 tttgctgggg ctattaattt ttgaaatata ttttttgaga ggcaaatatt caacggggga
    31621 agttattttt aaaagacaaa atattggcat tacggtagaa atagcgagca acagtttaac
    31681 tcagatgcaa ggtttaatgg ggcgaaagga attgccagaa ttttcaggta tgctttttgt
    31741 ttttcgcacc gaagcaccgc gtactttttg gatgaaaaat actcacatcc ccttagattt
    31801 aatttttatt tctcatgata aaaagattaa agaaattaag gctaactttg aaccatgtat
    31861 tgatagcaat aattgtccga gctatcaatc aaaagaaagc gcccaatatg ttttagaggt
    31921 gaatggtggt ttttgtgaaa ggcatcagat tgaagtaggc gatgaaatag aacttaaatt
    31981 ttagaaattt tactcaccgc tattttaaaa gttatccaca tcttgcatgg cgagttattt
    32041 ctgatagaat aaaagtagaa aataaaaacg gaataaaaat attttctaaa acagaagaag
    32101 gatgttagca gaaaaaattt ccgatcacct aaaaaattta gcgaagaaaa atccagcgat
    32161 caaacgacaa ttttaccttt cgaaatggga acttcataca aaagaagata aattaaattc
    32221 ggttgatccc cttttagaag acgaacacac taaaaaacgc ggtctcatct acaaatatcg
    32281 aggccgcgtt ttaattcttt tgacactttc ttgcgctgct tattgccgtt tttgtacgag
    32341 acgaagatgt gtttccgaaa ttgaaaaagg aattttaagc aaggaagata tagataatat
    32401 tgcgcaatat attaaacagc acaaagaaat taaagaaatt attttatctg gcggcgaccc
    32461 attaacagtg cctaatcttt tgaattattc tttaaaagtt ttttcagcct tgccgcaaat
    32521 aaaaattatt cggattggaa cgcgggtgcc ggtttctaat ccgaaattag ttactgataa
    32581 tcttttaaat cttattagaa aaatttctca acctgtttat gtgggcattc attttgaaca
    32641 tcctgatgaa ttaacgccag aaacaataaa ggcttgcgaa aaattacgca aagccggagc
    32701 cattctttat agtcagagtg tttttttaaa aggcgttaat gattcttatg aagttttata
    32761 cgaacttttt tctcgtctta ttgaaattgg agttaagcct tattatctct atcgttgcga
    32821 tcccgtgcag gggattgaac atttccgagt gccatttgaa aaagaaataa aaattgctac
    32881 gaaactaaga aaaaatttat caggattagc ttggccgact tatgtgattg atacgcctaa
    32941 tggcaatggt aaaattcctg tgcctttaga attttggcaa tttgataaaa aacaatttag
    33001 ggattttttg ggcagaaaac acgaagtaat ttaatagtta gggtaaattg agaaaaaaat
    33061 tgattgatgc ggcggaaaat tttcgaagaa aattttccgc cgctcgtttc gttttctctt
    33121 tgtcatttta cttgtctcgc cattctgttt tccattgtca tcctgttttt ttattgtcat
    33181 cctgagcgca gcctgcccgc cgaacaggcg gggctccgct cagaatgacg cactaccaca
    33241 tcgtcatccc gaggacttta gtccgaggga tccctcggct ttcagcacta attagcaatc
    33301 attgctgtgg atgggattct tcgggctgag gccctcagaa tgactgctgt ttttctttgt
    33361 catcctgaat tttagtgaag gatcccatgg ctttcagcaa taatcggcaa ttgctgctgt
    33421 ggatgggatt cttcgggctg aggccctcag aatgacagaa aataaaagcg ctcagaatga
    33481 caaaaaatgc tcagaatgac aattaaaaag gtggtcagga tgattaataa aaaaaccaga
    33541 caaatagaac aaaagaagca tgttttttaa ggcgttggta cgcagggtat tctcttgaca
    33601 aaatataaaa aataagtaat attcagtcag accttttaca aggctttata atagataatc
    33661 ttaaaaaatt aaaggaggtt ttcaatgaga aaacttagtt tggttgtatt gattgggttg
    33721 gttctgttga cgctgcctgt agcagctcag cagagccagg gtaaagagat tgagctgaag
    33781 actctaggac gggcgcctct cgcgccttat ctgcagaccg aagaggaggt tcgaattgtt
    33841 cttaagaagt acaacgaaga aattggtaag gcgttagagt atttcccagg cggagctctc
    33901 ggagaaatag tctatctcaa attgtacgaa aacgttgata gttttgttat gcaactgcag
    33961 cagattccgg tgggacaaaa gatggactgg atgatttatc ggaatggtgg tttgctaaag
    34021 aatgtgcgtt gggcgggaaa ttcatccttt gacgcttttg tttttgaagt agaagcagaa
    34081 ggaaagttgt ttcaatttgc tgttccgatt cgttgtggca acgtttcctt aattagcatg
    34141 aaagagattc ctatttctgc accagtagtt cagccacaac cacagcccca accgaaagca
    34201 gaacaaccat ttgttccgcc agcagttatt caagcaccag caccaacgcc tgcacctgcg
    34261 ttatctgcgc cgataaaagc gcagaaacgc tggtcaccca cggtcaaatt caaagtggga
    34321 agtggttggg caaaaacgag tatgccggca aatcaaaatc tggaaggtag agttgacttc
    34381 ttcacgctta ttccagatga aatgtctttg ttctactgtg aagatggaac agcagcatgg
    34441 ttatactcgc cgacgaagga tatcccgttt acattgggag aacaaattgg acttcatacc
    34501 caattgcaaa atacgtctat caggaagtat cagccgtttt ttgctggact agagctggag
    34561 atcgcgaaga acttctttgt gacggcagat tatttccaca tcgggaagtt ccacttgcaa
    34621 gagtatagtc tatccaatct catgtccgtt actgaattga gattgctcgg tgagtacgat
    34681 agtacaagtg gttctgcaga tggttgtact ccaacctacg tttattatat agggttggag
    34741 cggcagcggc gggagcagtt gatccaacag gatattcgga tagaagagtg gtctgttggg
    34801 gtaaagttcg agttgaagct ggcgaagaag ttctatgttg cgccagcagc aggagtgacc
    34861 tggcaagagc agcgtgggac gcgcgaggtg actgaagcgg ttactaaaat gtatcccttt
    34921 aaagaagaag ctctggaacc acagcaggtg gtttcaagtg ctgaaaagat caaaacttcc
    34981 gtcgcgcacc catacgtggg tgccagcgta gaactttatt gtttccaggt tgaagctaga
    35041 tatttgatga aaaaatttaa tgaagaagtt tatacctccc cctggcgcgt tcaaggcgcg
    35101 ctggttctaa gattttaaaa aaaataaagc aatcaaaagc ccattgacca aaggtcactg
    35161 ggctttttta ttttgacaaa attacttttc ttttattctt cttctatttc aattgcttcg
    35221 gcagcgcaag catcaatagc tgattgcaat ttctcttcat caattttttt aagttctttg
    35281 acttcggcct ttccgtcttc atcaagataa aacatttccg gatacataaa cgtgcagctg
    35341 ccgcagccca gacatttatc tttattaatt ttaatttttt tctttcccat tttttaaaat
    35401 ttaatccgac gctcttataa attatatttc tctttataga accgaatcaa ttcttccact
    35461 aaaggaatgc aactgggatc gcccaatccg actttggttt tttcttgcac ttcttctaaa
    35521 gttttagctc catccagcac tgctttttca atgtcttttt ccgtaatttt tagaatttta
    35581 tcaatgactc gactgccttc aggttcaatt ttgtcgtatt gtccagttct acgataataa
    35641 tcattaattg cgtcacgtaa ggccttgtca cctaaaactg agcagtgata tttaatatcc
    35701 ggtaatccac ctaattcttt gatgatatcg ttaggcgtaa tttttcgtgc ttgttctaat
    35761 ttcattcctc catggcgtaa aatcatctct gacaaaacag acgtggaagc aatagctgag
    35821 gcacagccaa aagtgcgcca gcgagcatct ttaatttttt cagttttcgg atcaactaaa
    35881 atccagaatt tcatcacatc accgcagacc ggactgccaa tcattcctag gccgttatac
    35941 tgaaatttct cttcttcccc gaatttcata aaatttttag gttgaagaaa atgttctttt
    36001 accgtttgac tataaaccca tggatttcct tttgtccaga tatcggcttc ttttttactt
    36061 ttttgttttt ttgatttttt agttagtgct ttctttgttt ttttcattga attttaatga
    36121 gattggtgat aattctcgta attccaaaac gacctttttt aatttttgga taagatagtc
    36181 aatgtctttt ttggtagtat ctttacccag cgtaattctt aaactgccat gaataaattc
    36241 agctggcaag ccaatagctt gaagaacatg ggaagggcta agacttttag aatggcaagc
    36301 cgagccagtt gaggcggcaa taccatattc atcaagcttt aaaatcaatg cctcgccttc
    36361 aacatccaat atagtaaaat gggcattatt cggcaggcga ttttttaaat caccatttac
    36421 aaaaacttta ggaatatttt ttaaaacatt tttaatgagg taattacgca aaagttttaa
    36481 tctttgcgat tcttgttttt tttctttttg cgctaatgtt aaagcagtag caaatccaac
    36541 aatacccgcg acattttctg ttccagagcg ataatttttc tcttggccac cgccataaat
    36601 aagaggtttg atctctacgc cctctttttt atataatacg cccgttgctt taggtccata
    36661 aatttttcct ccgttaaggc tcattaaatc aactcctaat ttttgagggc gaatatctaa
    36721 atagccagct gcttggcagg catcagtatg gaaaagagga aatatggttt tgtgttcagt
    36781 gcggaaattt ttgatgattt gagcaatttt gggaataggt tcaatgacgc caatttcatt
    36841 gttagcatac attattgaaa taagaattgt ttgatcgtca atcaaacttt ttaattggtc
    36901 caaatcaaca agtccgtttt tttggacaga gagataggca atttcaaaac cttgttcttt
    36961 taaagctttg actgtttcta atcctgctgg gtgttcgata gcagaaataa taattttagg
    37021 ttgagaaaat tctttttgat agtaatgggc cgtgccttgc aatgccaaat taacagattc
    37081 agtgccacca ttggtaaaaa ttatttcttt tgattgaact tgtagaatct ctgcaatttt
    37141 attacgggct gattcgatgg cttgtttggc tatacgacct tccaagtata aagatgaagg
    37201 attgccaaat tgtttttgcc aaaatggttt catggctgaa atcacttctt ttcttgttgg
    37261 cgttgtggcc gcatagtcaa aataatagcg cttcatacaa gttgggaaat ttttatccga
    37321 cttaatttta aagttatatc ttcggtaaga ccaacccagc aacttttaag cgtacaatga
    37381 gaagaattac aacgagaaga gcagttgatt aaggataaag gcccttcaag cgcttcaatg
    37441 acgcttaaga gcgaaatatt ttttggattg ttttttaagc gatagcctcc gttttttcct
    37501 cttttggatt ccacaaaatt atttttaatt aaagctcggt tgattttttg tagataataa
    37561 aaggaggtct tcgtttgatc acaaaaagag cgcaagctga caaattttaa tttgttcgtt
    37621 tgcgccaaat aaataagcca gcgaagagct agttcaattt gtttagaaat tttaatactt
    37681 ttgaaattca tactattaaa tacttatttt aataacatat aattaatcgt caagtagcaa
    37741 aacaaaaaga aaggacttaa gtcctttctt tttgttttaa taaattcaac ttatttcatt
    37801 gccgcgtacg tagcttgtac tttgagtcca ccgccgcctt cttcataaaa catttgaatg
    37861 ctcatcgaat ttctgctttt agtagcatag atattataaa taccgttgct gttagtatta
    37921 tttactatcg tccaaccatt ttgattaaga taagcaataa atttatcggc attttcttga
    37981 atggattttt gtgaataat
//
LOCUS       DAOSMC010000005        25745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_2429_length_25745_cov_17.321098, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000005 DAOSMC010000000
VERSION     DAOSMC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 25745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25745
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2429_length_25745_cov_17.321098"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PL075_01045"
     CDS             <1..1186
                     /locus_tag="PL075_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOD96818.1"
                     /translation="TYQELQKQIDAKKQTLNNLIEQQQFYNAELEKTQGTKRTLNNEL
                     NQIQKNLDNINFKIKINEVQIEKLNLELEQLQMDIGNTNNEMEIKKGVLAQNLQQLYE
                     KNRELTPLVIVLKNQKISDAFTEVNNIQQISESLKISLDELNNLKIALADRKNEAEQK
                     KEELEQTKIQLNNQKAIALNLQEEKKTLLSKTKNQEKNYQTLLSNLEKQRAEIELEVS
                     RLEEALKAQIDPSALPSARAGLLLWPIKNAVITQKYGATSDSQYFYNQGKYKTPSHNG
                     IDIKASVGTPVYAAEDGEVVATGNQDLYCYKGSYGRFVVIRHYNNLTTLYAHLSLIAV
                     KKGDIVKRGEIIGYSGNSGFTTGPHLHFTVYFSPTFQMSKSSSCGPMPIGGSVNPLPY
                     LE"
     gene            1257..>1495
                     /locus_tag="PL075_01050"
     CDS             1257..>1495
                     /locus_tag="PL075_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96819.1"
                     /translation="MFQKSKSSILISIGILIIASFVFVPSVLAEDNNANQSILPNFLQ
                     NFIKNMSRIADETIVPIINKTGVGDIIAEPINTET"
     assembly_gap    1496..1595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1596..1813
                     /locus_tag="PL075_01055"
     CDS             <1596..1813
                     /locus_tag="PL075_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96820.1"
                     /translation="NKTGVGDIIAEPINTETNESFLQRIKDWMTQTSQKINELFRVDI
                     GVGFAKIIDLFTYGFNQAVEWVVNLFK"
     gene            2156..3328
                     /locus_tag="PL075_01060"
     CDS             2156..3328
                     /locus_tag="PL075_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOD96821.1"
                     /translation="MHTSYTYLEVFENCPKRYEFQIIDKIKVPKTKEQFFGTLLHRCL
                     KFLFRKAPIYPTLDELLDYFSIQWKEADKVKWRDQKQKELFFNEGKRILEDFYKKNVP
                     SLSIPLDLEGRFEVEIEDEENNTKHTLAGYIDRVDKRDDGQFEIIDYKTAQKMPGQNT
                     LKNSLQLAIYTLGFFKKWPNLTSLDNIILTLYFLRHGEKLSDRQSADEIELTKKRVLK
                     DIHEIEKNYFPPIPSPLCSYCPYRQICPMWSHLYKKDVVPEEKEVNALIKEYFATKDQ
                     IDQQKEKLNIIKKQINDYAEKKGLERIFGEDGYFAYHNQVIKDYDTEQLKHLLMNAGL
                     WQSVSVLDKKKLEKLLKTLPPELIKQIESLEKIKNRKILKAVKKDIEIIKSELQED"
     gene            complement(3341..3496)
                     /locus_tag="PL075_01065"
     CDS             complement(3341..3496)
                     /locus_tag="PL075_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96822.1"
                     /translation="MQLKNALEKNKREIIIAVFVFLIVTTSFALGYLTATHFVQKTPI
                     VIEKCSE"
     gene            3576..3848
                     /gene="rpmA"
                     /locus_tag="PL075_01070"
     CDS             3576..3848
                     /gene="rpmA"
                     /locus_tag="PL075_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOD96823.1"
                     /translation="MAHTQSGGSTKLGRDSEAKRLGVKIFGGQKVKKGAIIVRQRGTK
                     IRAGKNVGIGKDHTLYALKNGIVQFSEKQKINFDGSKKRMKTVSVQ"
     gene            complement(3852..4298)
                     /gene="rplI"
                     /locus_tag="PL075_01075"
     CDS             complement(3852..4298)
                     /gene="rplI"
                     /locus_tag="PL075_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOD96824.1"
                     /translation="MKIILLENIPKLGKKYDIKNVSDGYALNFLIPKKLAVAATSQKI
                     KEIENLKKQEELKNQKIQALFKEWAEKIKNTTLDFRLKTDKSGKTFGSVTKEDIAKQL
                     EKTSGIKIEEKQIILDEHIKKVGDYVIKIKFSPEIETELKIHVLSE"
     gene            complement(4301..5638)
                     /locus_tag="PL075_01080"
     CDS             complement(4301..5638)
                     /locus_tag="PL075_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOD96825.1"
                     /translation="MNSKKPEESSKKLLANESDELKIESDSKEKKTESKNKLSWKRIV
                     KIGGIIILCAIILGAIFISGVEIGKRFYPSINLIHGVKNLETNKPQNVDFSLFWDAWR
                     YVEEYYAKANELDYQKMVYGAIKGMVDSLGDPYTVFFEPDDAKKFTEDVSGNFSGIGI
                     EIGIKNNSITVISPLEGTPAWEVGLRAGDKILAIDQTSTDSLTLDEAVKLIRGEKGTN
                     VTLTIMREGFNEPKDFTIVRDIINVPAVKLTFLDNNIAHLELLNFNENATEEFNRAAT
                     QIIMKKSPGIILDLRNNPGGYLEISVDIAGWFLKKGDVVVRERENDNREKLFRASGNQ
                     ALLNIPMVIITNEGSASASEILAGALKDNRNIKIVGTKTFGKGSVQTLLPLEDNSMIK
                     ITIAEWFTPNGSIINDVGLTPDYEVELTEEDWESNRDPQLDKAIEILKNEIES"
     gene            complement(5685..6236)
                     /gene="thpR"
                     /locus_tag="PL075_01085"
     CDS             complement(5685..6236)
                     /gene="thpR"
                     /locus_tag="PL075_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOD96826.1"
                     /translation="MKNNYLRLFFAIELSADIKDKLVAVLKKMPLPERVNLTPKENLH
                     LTLLFLGSVSLDQLPQIIEIAENVFARYSIFDLYFNNITGAPNENYCRMIWLNGENSP
                     ILEHIKNDLESELEKNGINFEKENRSLKIHITLARFNPMRCSFQPIEFPERLRVKELV
                     LMESQLQKPHAVYIPLKKFPLRP"
     gene            6293..8194
                     /gene="priA"
                     /locus_tag="PL075_01090"
     CDS             6293..8194
                     /gene="priA"
                     /locus_tag="PL075_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOD96827.1"
                     /translation="MFLVNVIPIAYLPRSQNKTLVYFSSQRQEIYSLVFIPFGRRKTE
                     ALVLSCEEIQSKIAIKKAEFQIKPLGKTINPNPIIHQKQWELFDWLSNYYFNPLASLL
                     KYSLPSLNLLKQINIKNSPAAPADQLLKNNPPLLPQVLFNDDFFLLKPAIQQTLDDNK
                     QVLFLFPNQIKLEYYWQHFSEYQEQILIYENKNTKKFPKIYQEINDGKTKIIFGKRSA
                     LFVPFYNLGLIVVVDEQSAGHETIETKIHYNAKQAAIKLQNLWQAQLVFTSYAPSVET
                     TFKINNGKYHCLNHPVMPDAQKISIIHKDAKQNNIFADQTQTILEKALKNESRTLIFN
                     NRRGHSPALVCQTCGFVFQCPHCDTALVYHKNDVKTLTCHHCGYTELAPNFCPNCQSY
                     LIQFLGIGNQKINEYLKHSFPNVKSAIFDSDHLKNLRQEKGLFDEFMNKKLDVLIATE
                     LFSKFFDFISSIDLIIIPSIEQMLVTPDYRTEERIRNLLTIFSLKSKRVLIQTFDPAK
                     KWLTEILEKDFYERQIEMRKKTFYPPFSQIIKIEIAHKKPAQLLKLSDYTYKLLEKNL
                     ARLFKPDHYIISHPISPVVPKKNNIYFKNIYWRLIAHQESNILLKRNAVLKLLPDEVN
                     VEIEPFDLF"
     gene            8215..8694
                     /gene="def"
                     /locus_tag="PL075_01095"
     CDS             8215..8694
                     /gene="def"
                     /locus_tag="PL075_01095"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOD96828.1"
                     /translation="MRILSIFDKQDEAFLRQKTQEVKDPTDPEIRKLIETMKDAMKKN
                     NGVGLAANQIGKSWRIFIAEYQNQFYAFINPRIIKHSRKIVLGEEGCLSVPYIVGIVP
                     RYEKIVIEGRDLNNNKIKIKASGTLARIFQHENDHLDGIIFIDKAKELYQVKSAKND"
     gene            8687..9634
                     /gene="fmt"
                     /locus_tag="PL075_01100"
     CDS             8687..9634
                     /gene="fmt"
                     /locus_tag="PL075_01100"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOD96829.1"
                     /translation="MTKNEVKIVFWGTPYFAARILEGLISAHFCPILVVTAPDKVKGR
                     GLQKTPSEVKKIAKKWGLEVVEPAKLRQNEAFLQQLRQLDPDLFIVAAYGKIIPAEYL
                     SIPQKGALNIHPSLLPRWRGPSPLQATILAGDTETGVSIILMDEEMDHGPIIKNSKLN
                     SPIAKLTYRQLEEKCIEEAIKLLIEILPQWLNGEINPIPQDHEKATYCKIIKKEDGLT
                     DFNEPAELIERKIRAFEVWPNVYFERNGKTYKILEADIFKDENILKNKTLGEFFCLNK
                     QLIVKCADNGLLIKKIQPENKKPLDGYAFWCGYQNKIAK"
     gene            complement(9710..9928)
                     /locus_tag="PL075_01105"
     CDS             complement(9710..9928)
                     /locus_tag="PL075_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96830.1"
                     /translation="MKPQIEEIRFRIGSFVLWFVPVPTKNGKKQPKLKYWARDSIIIE
                     NPDLSKKDIPAEALREAYARAAVIFNKF"
     gene            complement(10293..11345)
                     /gene="mraY"
                     /locus_tag="PL075_01110"
     CDS             complement(10293..11345)
                     /gene="mraY"
                     /locus_tag="PL075_01110"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOD96831.1"
                     /translation="MSFELIILQLSRLFILTTVSFVLALLLTPVLLKILSKYNVGKQI
                     RAEGAPVFASFHQKKEGTPTMGGILIWFTTAIIALIVFLLSKIFDQVFDYFNFVSRAQ
                     TYLPLFSLIGAALIGLADDILGVLKRGPKGGGLSVKQKLILYFIIALVGASWFYFKLN
                     WDVIHIPFLGDISLGPWYILFFIFVVAGTAHATNLTDGEDGLAGGVMLFAYLAMAVVS
                     FVQGRFDLATFIGVILGALLAFLWFNIHPARFFMGDTGSMALGITLGVLAMYTNTAFL
                     LPFFAFIPLVETLSVVVQLISKKFRKKKVFLSSPIHHHFEAIGWPEEKITMRFWIISA
                     ISTVFGLVIFFIDKIL"
     gene            complement(11335..12327)
                     /locus_tag="PL075_01115"
     CDS             complement(11335..12327)
                     /locus_tag="PL075_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOD96832.1"
                     /translation="MEVFKKIIKIMIIVLVGVGIGFGGSWLYARFFINENFRTNGSLF
                     LEQYSVRSNPSAITYPFRNWDTQDDPLVQAEGALVVDLDSDFIFYQKQPNLRRPIASL
                     TKLMTALIAEKYLPSNEEVVISEEALNIDGDPDIFYQQERLTVNDLIQSLLIISSNKA
                     AKALSLHMGEDRFVGLMNDMAKQLGMTQTTFVEPTGLSFLNQSTPSDLKKLMRYIVNE
                     YPNLLIISKTAETKIQGVYEQQDKSATVEHNLFNINLIAHNPKFLDELNVVYAGGKTG
                     FTDEAWQTFCGVFLVPSQRSNESPRRILVVVLKTPDRYNDIETLLRWLNKAYVF"
     gene            complement(12330..12848)
                     /gene="scpB"
                     /locus_tag="PL075_01120"
     CDS             complement(12330..12848)
                     /gene="scpB"
                     /locus_tag="PL075_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOD96833.1"
                     /translation="MNSEELARKIEAILFIAGEPVTFQKLADTLEIPLEEIKKTVQVM
                     QNEYQNNRRGLSLVIFDEQVQLTTAPDLRESIEKFLQKILKEDLTPAALETLSIIAYK
                     GPISRAEIDNIRGVNSSYILRNLLIRGLITREIDPHRANAFVYKVSFDFLRKMGLEKI
                     EALPDYEKYHNL"
     gene            complement(12838..13572)
                     /locus_tag="PL075_01125"
     CDS             complement(12838..13572)
                     /locus_tag="PL075_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOD96834.1"
                     /translation="MNQQKINQLEIQQEKFSGPLDVLLSLIEERKMEITEISLAQVTD
                     AFLNYVGDLKQSSPDILADFLVIAAKLILIKSKSLLPTLELSPEEEIEIKDLTERLEI
                     YRLFKNASQFIKENFTQQQMFAREFLVNQSPIFYPPENCTPQMLYNSFVKIWQEFSSL
                     GETTIEKIKKVIKIEEKIKNILKILTERTSSEFNQLVSVKEKIEVIVTFLAVLQMFKE
                     QLIIIDQQENFGEIKITIKQSHHYEQ"
     gene            13648..14004
                     /locus_tag="PL075_01130"
     CDS             13648..14004
                     /locus_tag="PL075_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96835.1"
                     /translation="MAIRYGRKNCHHEWAIRADGYEEGCAPIICIKCGAFGCLCDARR
                     EFRKWYFIKKMGIPASLYNRLQFSMIIPDDYKARQKEQKLFDLIFSRKYNSTDNINQK
                     WKNPYVKKLKTEKNKK"
     gene            complement(14493..17108)
                     /gene="secA"
                     /locus_tag="PL075_01135"
     CDS             complement(14493..17108)
                     /gene="secA"
                     /locus_tag="PL075_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOD96836.1"
                     /translation="MRLNFFHSDLSQKFYKQNKKFIDKVNAFESELVTLSDFSLREKS
                     LSLREKVKSPADLENVLIEGFALVREAAKRALKQRHYDVQLLGGLALHQGKIAEMKTG
                     EGKTLAATLPVYLNALTKKGVHVVTVNDYLAKRDTVWMGQVYYLLGLSIGCIAHDEAF
                     LYDPDYKNVNNSANILINNQNNNKEKGDETDEKRDATGSFKVFYEYLRPVSRKTAYDA
                     DITYGTNNEFGFDYLRDNLVYDLNQKVQRGHYYAIVDEVDSILIDEARTPLIITIPDM
                     EASESYKLFAKIVPRLEKNIDYQVDEKLKSVLILDSGIEKVEKMLGVKNLYSPENFRY
                     VHYLEESLKAYALFKRDKDYIVKDGEVIIVDEFTGRLLFGRRYSGGLHQAIEAKEGVE
                     IKQENRILASITFQNYFRMYEKLAGMTGTALTAAEEFDKVYGLEVVPIPTNKPMIRKD
                     LPDLVYKTSTEKWEAIVEEIKKRHNIGQPLLIGTVSIENNEKLSAMLSREGIPHNVLN
                     AKNHEKEGEIIAQAGKLKAVTVATNMAGRGVDIILGGNPPIAEEVAKVKELGGLHVIG
                     TERHEARRIDDQLRGRAGRQGDPGSSQFFVSLEDDLMRIFGGERIKSLMGVLNYPYGM
                     PIQSKIVNRAIESAQTKIEGLNFDARKYTLEFDDVLNKQRRVFYDYRNKILDLAQSHH
                     LLSEVESIIEKNIARLINVPIENLEAFLKEFKIIEENVKLNEIAESSLYNFLLEKSKT
                     AFERIKAEIGIELLENISKSMMLNVLDNLWTAHLENLEYLREAVRLRAYGQHDPLVEY
                     KTNAFRLFKDFFDRFEEMFFEIIFQIKKQQVSPVAKPAPSLPSDISPEKFKNVGRNDP
                     CPCGSGKKFKKCHGR"
     gene            17222..17806
                     /locus_tag="PL075_01140"
     CDS             17222..17806
                     /locus_tag="PL075_01140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOD96837.1"
                     /translation="MVKINNNYFLLRHGEAFSNREPRWLSSWPEIKIAHLTSRGKLKI
                     KKQAQIFKTKGIDLIFSSDLARTKETAQIVAQIIKKPVIFKKELRELNFGVFNGFHPN
                     VYWAYFQNPLERFTKKIPHGETLNQCAKRIFNFWKKINCRYQNKNILIISHADPLWLL
                     ESKIRKLTKIQMVKNYRLRLSPGEVRIIRAHSKD"
     gene            complement(17796..18155)
                     /locus_tag="PL075_01145"
     CDS             complement(17796..18155)
                     /locus_tag="PL075_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96838.1"
                     /translation="MTKDELKIFLHDRWVRISLIINIIILLVALVIIFLNIKFLPQRS
                     YILYYTSLEGIKMTGYFWNFYLFWFVGFFFSALHLLLAFIFWFRIKKLAQFILASNIL
                     INIFLFLAIVAMVMVNL"
     gene            complement(18260..18334)
                     /locus_tag="PL075_01150"
     tRNA            complement(18260..18334)
                     /locus_tag="PL075_01150"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18299..18301),aa:Glu,seq:ctc)
     gene            18461..19039
                     /locus_tag="PL075_01155"
     CDS             18461..19039
                     /locus_tag="PL075_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96839.1"
                     /translation="MNRKVRFLKWTPQTKIDAVIALGMGFKRDFTDISDQTKSICSTA
                     ALISIQNNIPLVLVGQEMDDEQEETAKCVPNESVKMYRFVRYLHTEQNIVEKISQLNA
                     RSLYGKLDTVLELVEKTGWEKIAIICHVDMMPRIKKVLKQLGLMKEVEVSVYLAPIRP
                     MYNGDSDFWWLRENWRAKLYEMSSSLFNLINR"
     gene            complement(19072..20310)
                     /gene="murF"
                     /locus_tag="PL075_01160"
     CDS             complement(19072..20310)
                     /gene="murF"
                     /locus_tag="PL075_01160"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOD96840.1"
                     /translation="MKSPLLFNTLKNILRILAQWTLKKYKPAVIGITGSVGKTSTKEA
                     VYTVLKGERNVRRSQGNFNNELGVPLTILGDYNAIRGILFWPKVIFQAIGQLLIFNKK
                     YPEILVLEMAADRPGDIQYLARLARPKIGVITSIGEIPVHVEFYSSPTAVAVEKARLI
                     ELLPANGFAILNRDDELVWNLRIKTRAHIISYGFKEDADIQIINFEYKFDENEFPEGI
                     TFKIQYGNVFVPFRLFRVMGKPQIYSIAAAVAVGIVFGINLVKISDYLANYQPPDHRM
                     QIIKGIKNSVILDDCYNASPLSMKAALETLGHFTQRRRVAILGDMLELGKYTTEAHEE
                     IGKEVIKKVDFLICVGSAARLIAESAIKHGFDKNKVLVFNNSESASKIIKKEISSNDA
                     ILIKGSHAMQLEKIVEELKK"
     gene            complement(20288..21991)
                     /locus_tag="PL075_01165"
     CDS             complement(20288..21991)
                     /locus_tag="PL075_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOD96841.1"
                     /translation="MKRKKAKQFLNNWRLHFILIIFVLSAGIILWRLYSLQILHYEDF
                     SQKAAAQVKTAEQIKNNLQYLRGTIYFQTREGSLIPVAVNKKFFEIYAVPKEIDDPVA
                     AASVLSKQLNLSYEDLIIKFSKKQSLYELLTKKVDETTAQKIEDLNLKGIYTKEYIGR
                     YYPYKEFASQLIGYVKDDVKNFHGQYGLEKYYDDYLSNQSLSNDLFDFLSLRNLIYSR
                     ANNDIVTTIDFHIQNKAEDLLEKKAKEWKAEGGSIIVMEPETGKVLTVANYPNFDPNR
                     YSSYSLNSFLNSAIQLTYEPGSVFKIITVAAGLDSQRITPQTKFYDNGEVVVSGKTIK
                     NWDFKAHGECNVFDIIAYSLNTGAVYLEKLIGHDIFHQHVLNFGFNQKTGIDLPNEAV
                     GNINNLKSFNDIYFATASFGQGISVTPIAMINAFSTIANNGLMMTPYVVETIVDPEGN
                     KEKTFEPKPKKQVISKGTADQLRIIMVNSVEKNKVAEIQGYQIAGKTGTAQVPDANGT
                     YSDKTIHSFIGYAPADDPRFIILVKLDKPEASLAGSTVVPMFKELAQFILDYYEIPPA
                     I"
     gene            complement(22004..22405)
                     /locus_tag="PL075_01170"
     CDS             complement(22004..22405)
                     /locus_tag="PL075_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96842.1"
                     /translation="MTIISLKHNRYNLVTQRMNWQKKNSKVLNSRFSFVAFLNIGLMI
                     LIIFTIVGSLFLEIKMVNYRFDIKNLTSQSVVFEQKNAELKNQVASLQSFDKFTAIAE
                     KEGLILVAVPGYFSLVKNPQMEVGLTNLNNL"
     gene            complement(22402..23283)
                     /gene="rsmH"
                     /locus_tag="PL075_01175"
     CDS             complement(22402..23283)
                     /gene="rsmH"
                     /locus_tag="PL075_01175"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOD96843.1"
                     /translation="MHIPALLQEVIQFVDPQPNENFIDATLGEGGYTLAILQHTAPLG
                     KVLGIDLDSEVLKVAERRLVAFKDRVILRQGNFKNIARIAQKEKFKPVNGIVFDLGLG
                     SFQIEESQRGFSFLRDEPLKMTFDSQGSLTAEEVINKMSVNELTKIFREYGEEKNAYR
                     IAKTIVEERKKRRITTTSQLVEIVTKTGRRKTKIHPATKIFQALRIYVNDELNNLTEA
                     LKGSVKILDVKGRIAVVSYHSLEDRIVKNFFRDKQKEGVLKIITKKPVTPTKVEILNN
                     RRCRSAKLRVAQKIKQS"
     gene            complement(23287..23718)
                     /gene="mraZ"
                     /locus_tag="PL075_01180"
     CDS             complement(23287..23718)
                     /gene="mraZ"
                     /locus_tag="PL075_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOD96844.1"
                     /translation="MLIGEYHYNLDTKGRLALPAKLRHELGETVIITRGLDSCLFVYP
                     KKEWEVLLSQLVNLPLTQSNSRAFSRFMLAGAVESEIDQQGRVLIPDYLRKFAGLNKK
                     VVIAGVSNRLEIWDENLWNEYRIKTERDANDIAEKLINFGI"
     gene            complement(23951..24331)
                     /locus_tag="PL075_01185"
     CDS             complement(23951..24331)
                     /locus_tag="PL075_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96845.1"
                     /translation="MRFKIPQNSETVVWTKHSIEKMMQYQLSESRIKRVLHTPHRIEE
                     GIAPQTIAVMQNAGTIKHPKEIWVMLQEIKTKQNKKDKKISAGLSHQKIRIISVWRYP
                     GKTPIRELPKLPPEEWEFLQKGDF"
     gene            complement(24335..25492)
                     /locus_tag="PL075_01190"
     CDS             complement(24335..25492)
                     /locus_tag="PL075_01190"
                     /inference="COORDINATES: protein motif:HMM:NF017380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOD96846.1"
                     /translation="MIYRGIATFGLDEGKCPRWLFERMTKLGREIIEVIINEYGSDEF
                     IKRISDPVWFQSLGTVLAFDWNSSGLTTILTAALKEAIRGREKELGIFICGGKGKTSR
                     RTPEEIEQWGNALSLPDGYVNSLIYNSKMTAKVDSALVQDGFQIYHHTLFFSKNGSWT
                     VIQQGMNIQNQSARRYHWYSKKITDLVKEPHAGIASQILNQPTLNLTALKSEKTRSVS
                     TQLVQNNYYDLMKDIKILAKHFSDYSQVLKVKSDGQQTIFLNLENREFVWHPVVKENF
                     FKNKYLQKILYLVCEQRPASYEKLLSLKGVGPKTIRALSLVSEIIYGAKPSYRDPARY
                     SFAHGGKDATPYPVDRITYDQSIKFFADVVSKSRLQSSEKNRIFYKLKISK"
     gene            25569..25643
                     /locus_tag="PL075_01195"
     tRNA            25569..25643
                     /locus_tag="PL075_01195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25604..25606,aa:Val,seq:cac)
BASE COUNT         8681 a         4651 c         3864 g         8449 t
ORIGIN      
        1 tacttatcaa gagcttcaaa aacaaattga tgccaaaaaa caaacgctta ataatctaat
       61 tgaacagcag caattttata atgcggaact tgaaaaaaca caaggaacga aaagaacttt
      121 aaacaatgag ttgaatcaaa ttcaaaaaaa tcttgataat atcaatttca aaataaaaat
      181 taatgaggtg cagatcgaaa aattaaattt ggaattagaa caattacaaa tggatattgg
      241 caataccaat aatgaaatgg aaataaaaaa gggtgttttg gcacaaaacc tccagcaatt
      301 atatgaaaaa aatcgtgaat taacgccatt ggttattgta ttaaaaaatc aaaaaatatc
      361 ggatgctttt acagaagtaa ataatattca gcaaattagt gagagtttaa aaatttcttt
      421 agatgaatta aataatttaa aaatagcgct tgctgatcgc aaaaacgaag cagagcaaaa
      481 aaaagaagaa ttagaacaaa ctaaaataca attaaataat caaaaggcta ttgctttaaa
      541 tctgcaagaa gagaaaaaaa cattactcag caaaaccaaa aatcaagaaa aaaattacca
      601 gactcttttg agtaatctag aaaagcaaag agcggaaatt gaattagagg tttcgcgatt
      661 ggaagaagca ttaaaagctc aaattgatcc ttcggcatta ccgagtgctc gagcgggttt
      721 gttattatgg cctatcaaaa atgctgttat tactcaaaaa tatggcgcta caagtgattc
      781 tcagtatttt tataatcaag gaaaatacaa aacgccttct cacaatggta ttgatattaa
      841 agcaagcgtt ggaacgccag tttatgcagc agaagatggg gaggttgtag caacaggaaa
      901 tcaagattta tattgctaca agggttctta tggtcgtttt gttgttatta ggcattataa
      961 caatttaaca actctttatg ctcatctctc cttgattgct gtaaaaaaag gtgatatcgt
     1021 aaaaagaggg gaaattatcg gttatagcgg aaattctgga tttactaccg ggccccacct
     1081 tcattttacc gtttattttt ccccaacttt tcaaatgagt aaaagttctt cctgcggccc
     1141 aatgccaatc ggtggatctg ttaatccatt accgtattta gaataaaaaa tgcctatttt
     1201 ataaaaaatt gtgcgacata tttaattaaa attgtgcgac ataaaaattt aatcatatgt
     1261 tccaaaaatc caaaagttca attttaataa gtatcggcat tttaataata gcaagttttg
     1321 tttttgtgcc gtcggtttta gctgaagata ataatgctaa ccaatcaata ttgccaaatt
     1381 ttttacaaaa ttttattaaa aacatgagtc ggattgccga tgaaacaatt gtcccaatca
     1441 ttaataaaac cggcgttgga gatattattg ctgagccaat aaatacagaa actaannnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttaat aaaaccggcg ttggagatat
     1621 tattgctgag ccaataaata cagaaactaa tgaaagtttt cttcagagga ttaaagattg
     1681 gatgactcaa acgtctcaaa aaattaacga gttgttccgt gtagatattg gcgttggttt
     1741 tgctaaaatc atagatcttt ttacttatgg atttaatcaa gcggtagaat gggttgtaaa
     1801 tttattcaaa taaaatagaa ttttagaagg gagccggcag caattttctt tgaaaattgc
     1861 tgccggcttt tttaacccca aaaagcatta aaaggggtgt ttttatcaaa cctataataa
     1921 aaattaaagg cttagtttat tatatgtgtc gcaaaatcta ccttgtgcga cacataacta
     1981 ataataaaac aaggcttttt tattctcccc tctattaaga ggggtagaaa aaacgccctg
     2041 gtttgtgaca gaaatcttct ttaaaaagat ttctgtcaca aataagaacc tttgtcggcg
     2101 aatattttgt tttaaaattt ttgatttttg ttttttgatt ttttataata tcgttatgca
     2161 tacctcgtat acttacctag aagtttttga aaattgtccc aaacgttatg aatttcaaat
     2221 tattgataaa attaaagttc ctaaaactaa ggaacaattt tttggcacac tcttgcatcg
     2281 gtgtttaaag tttttgttta ggaaagcgcc tatctacccc actcttgatg aattactcga
     2341 ttatttttct attcaatgga aagaagccga taaagtaaaa tggcgcgacc aaaaacagaa
     2401 ggaacttttt tttaatgagg gaaaaagaat tctggaggat ttttataaaa aaaatgttcc
     2461 ttctctttct attccgctag atttagaggg tcgttttgaa gtagaaattg aagatgaaga
     2521 aaataatacc aaacatactc ttgctggtta tattgatcgc gttgacaaaa gagacgatgg
     2581 ccagtttgaa attattgatt acaaaacagc ccaaaaaatg ccgggtcaaa ataccttaaa
     2641 aaatagtctt caattagcaa tttatacctt aggcttcttt aaaaaatggc ccaacctcac
     2701 aagtttagat aatattattt taacacttta ttttttaaga cacggtgaga aattatccga
     2761 tcgtcaaagc gctgatgaaa ttgaattaac taaaaaacgt gttttgaaag atattcatga
     2821 aatcgaaaaa aattattttc ctcccatccc ctcccctctt tgttcctatt gtccttatcg
     2881 acaaatttgt cccatgtggt cacatctcta taaaaaagat gtcgtgccag aagaaaaaga
     2941 agttaatgct ttaattaaag aatattttgc tacaaaggat caaattgatc aacagaaaga
     3001 aaaactcaac atcatcaaaa aacaaatcaa tgattatgct gaaaagaaag gtttggaacg
     3061 catttttggc gaagatggct attttgccta tcacaatcag gttatcaaag attacgatac
     3121 agaacaatta aaacacttgt tgatgaacgc tggattgtgg cagagtgttt cggtattaga
     3181 taagaaaaaa ttggaaaaac tgcttaaaac cctcccgcca gaattaatca agcaaattga
     3241 aagcttagaa aaaattaaaa accgcaaaat tttaaaagca gttaaaaaag atattgaaat
     3301 aattaaaagc gaattgcaag aagattagaa ggttttgttt ttattccgaa cacttctcaa
     3361 taacaatagg tgttttttga acaaaatgag tggcggttaa ataacccaat gcaaaactgg
     3421 ttgtaacaat taaaaacaca aaaacagcta taataatttc tcttttattt ttttctaaag
     3481 catttttgag ttgcattttt tttaattttt tttaaaatat catctaacaa ctgtcgtata
     3541 ttattagaat tatttatatt atttaattca aacttatggc tcatactcaa tccggtggtt
     3601 ctactaaatt aggaagagat tcagaagcaa agcgcttagg cgttaaaatt tttggtggtc
     3661 aaaaggttaa aaaaggcgct attattgttc gacaacgagg aacaaaaatt cgagctggta
     3721 aaaacgttgg aattggcaaa gatcatacac tctatgcatt aaaaaatgga atcgtccaat
     3781 ttagcgaaaa acagaaaatt aattttgatg gttcgaaaaa acgtatgaaa acagtttccg
     3841 ttcaatagtt attattcgga taaaacatga atttttaatt cagtctcaat ttcgggactg
     3901 aatttaattt ttatcacata atcccccacc ttcttaatgt gctcatctaa aataatctgt
     3961 ttttcttcaa ttttaattcc actcgttttt tctaattgct ttgcaatgtc ttctttggta
     4021 acagaaccaa aagtcttgcc gcttttatca gtttttaagc gaaaatcgag tgttgtattt
     4081 ttaatttttt cagcccactc tttaaatagt gcttggattt tttgattttt taattcttcc
     4141 tgttttttta aattttcaat ttctttaatt ttttgagaag tcgcagccac tgctaatttt
     4201 ttgggaatca aaaaattaag cgcatagcca tcggaaacgt ttttaatatc gtattttttc
     4261 cctaatttag gaatattttc caaaagaatt attttcataa ttaactctca atttcatttt
     4321 ttaaaatttc gattgcctta tctaattgag gatcgcgatt agattcccag tcttcttcgg
     4381 tcagttctac ttcgtaatct ggcgtaagac cgacatcatt aataatagaa ccgttgggcg
     4441 taaaccattc ggcaatagta attttaatca tagaattatc ctccagaggt aaaagcgtct
     4501 gaactgatcc tttcccaaag gtttttgttc caacaatttt tatattacga ttatctttta
     4561 aagcgccagc caaaatttct gaggcagaag cagatccttc attcgtaata ataaccatcg
     4621 gtatattaag cagggcttga ttacctgaag ctcggaataa tttttctcga ttatcatttt
     4681 ctctttctct taccactaca tcgccttttt ttaaaaacca gccggcaata tcaactgaaa
     4741 tctctaaata accaccggga ttattacgta aatcaagaat tattcctggc gatttcttca
     4801 taattatttg agtggcagcg cgattaaatt cttccgtcgc attttcatta aaatttagta
     4861 attccaaatg agcaatattg ttatcaagaa aagttaattt tacagccggt acattaataa
     4921 tatcacgaac aattgtaaaa tcttttggtt cattaaagcc ttctctcata atagttagag
     4981 tcacgtttgt ccccttttct ccacgaatga gcttaacagc ttcatctaaa gttaaactat
     5041 cagtggatgt ttgatcaata gcaagaattt tatcaccggc ccgaagtcct acttcccatg
     5101 ccggagttcc ttccaacggt gaaataactg tgatgctgtt attttttatt ccaatttcaa
     5161 taccaattcc actaaaatta ccactgacat cttctgtaaa ttttttagca tcatctggtt
     5221 caaaaaatac tgtgtaagga tctcctagcg agtctaccat acccttgata gctccgtaaa
     5281 ccattttttg ataatctagc tcattggctt tagcgtagta ttcttccaca taacgccagg
     5341 cgtcccaaaa aagcgaaaaa tcaacatttt gtggcttatt ggtttccaaa tttttaacac
     5401 cgtgaataag gtttatcgaa gggtaaaacc ttttcccaat ctcaacgcca ctaataaaaa
     5461 ttgcgcctaa aataatggca caaagaataa taatacctcc tattttaaca attcgtttcc
     5521 acgataattt atttttagat tctgtttttt tctctttaga atcggactcg attttcagct
     5581 catcagattc gtttgctaat aatttttttg aagattcctc tggtttttta gaattcatag
     5641 taataaaatt attataactt tttattttgt ctttgcaatt ctatttaagg acgcaaagga
     5701 aattttttta aaggaatata aaccgcatgg ggtttttgta attgactctc cattaaaaca
     5761 agttctttga ctcgtaaacg ctcgggaaat tcaatcggtt gaaaactgca tctcatcgga
     5821 ttaaagcgag ccagcgtaat atgaattttt aatgatctat tttctttttc aaaattaata
     5881 ccattttttt ctaattctga ttctaaatca tttttgatat gctctaaaat cggagagttt
     5941 tcgccattga gccaaatcat tcgacaataa ttttcgttag gcgctccggt aatattatta
     6001 aagtataaat caaaaatcga atatcgagca aaaacatttt cggctatttc aataatctga
     6061 ggcaactgat ccaaagatac tgaacctaaa aataatagcg tcagatgcag attttcttta
     6121 ggagttaaat taactcgttc aggcaagggc atttttttta aaacagcaac aagtttatct
     6181 ttaatatctg ctgaaagttc aatagcaaaa aataatctta aataattatt tttcattctc
     6241 ttcattttat cgctattttt tatatttgtc tatttttgat tataataaaa ttatgtttct
     6301 agtaaatgtc atccctatcg cctatcttcc tcgatcacag aataaaactc tcgtttattt
     6361 ttcttctcaa agacaagaga tctatagttt agtttttatt ccttttgggc gtcgaaaaac
     6421 tgaggcttta gttctttctt gcgaagaaat tcaatcaaaa attgctatta aaaaagctga
     6481 atttcaaatc aaacccctcg gaaaaacaat aaatcccaac cccatcattc atcaaaaaca
     6541 atgggagtta tttgattggc tttccaatta ttattttaat cccttggcct ctcttctaaa
     6601 atacagcttg ccttctttga atctactaaa acaaattaac atcaaaaact cgcctgcagc
     6661 gcctgctgat caacttttaa aaaataaccc gccccttctt cctcaggttt tatttaatga
     6721 tgattttttt ctattaaagc ccgctattca acaaacatta gatgacaata aacaagttct
     6781 tttccttttt cctaatcaaa ttaagttaga atattattgg cagcattttt ctgaatatca
     6841 agagcaaatt cttatttatg aaaataaaaa taccaaaaag tttccgaaaa tttatcaaga
     6901 aattaatgat ggaaaaacta aaattatttt tggcaaacgg tctgctcttt ttgtcccttt
     6961 ttataacttg gggctaattg ttgttgttga tgaacaaagc gccggtcatg aaacaattga
     7021 aacaaaaatt cattacaacg caaaacaagc agctataaaa ttacaaaacc tctggcaagc
     7081 tcaactcgtt tttacttctt atgcgccttc cgtagaaact acatttaaaa tcaacaatgg
     7141 caaataccat tgtttaaatc atcctgttat gcccgatgca caaaaaattt ctattattca
     7201 taaagacgct aaacaaaata atatttttgc agatcaaact caaacaattc tcgaaaaagc
     7261 attaaaaaac gaaagccgaa cgttgatttt taataatcgt cgcggccatt cacctgctct
     7321 cgtttgccaa acttgtggat ttgtcttcca gtgccctcat tgtgatacag cccttgttta
     7381 tcataaaaat gacgtcaaaa cacttacttg tcatcattgc ggttatactg aactcgctcc
     7441 caatttttgt cccaactgtc aaagttatct cattcaattt cttgggattg gcaaccaaaa
     7501 aataaatgaa tacctgaaac attcttttcc taatgtcaaa agtgctattt ttgactccga
     7561 tcatttaaaa aatcttcggc aagaaaaagg actatttgat gagtttatga acaaaaaatt
     7621 agacgtttta atcgcgacgg aacttttctc aaaatttttt gatttcatct cttcgattga
     7681 cctgattatt attccttcaa ttgagcaaat gctcgttacg cccgattata ggactgagga
     7741 gcgaattaga aatctcttga caattttttc tttaaaatca aaacgggtat taattcaaac
     7801 atttgatcca gctaaaaaat ggctaaccga aattttagaa aaagattttt acgagcgcca
     7861 aattgaaatg cgcaaaaaaa cattttatcc ccccttcagc cagattatca aaatagaaat
     7921 tgcccacaaa aaaccggctc aattattaaa attatccgat tacacttata aactgcttga
     7981 aaaaaatctt gcacgcctct ttaaacccga ccattatatt atttctcacc ctatttcgcc
     8041 tgttgttcca aaaaagaata atatttattt taaaaatatc tattggcgac ttattgctca
     8101 tcaagaatca aatattttac ttaaacgtaa tgctgtttta aaattattac ccgatgaagt
     8161 caacgttgaa atagaaccat ttgatctttt ttaatttttg ctatactcat aataatgaga
     8221 atattatcaa tttttgacaa acaagacgaa gcatttcttc gccaaaaaac acaagaagta
     8281 aaagatccga ctgatccaga aatcagaaaa ttaattgaaa caatgaaaga cgcaatgaaa
     8341 aaaaacaatg gcgtaggatt ggcggctaat caaatcggca aatcatggcg tatttttata
     8401 gctgaatatc aaaatcagtt ttatgctttt atcaatccaa gaataataaa acactcgcga
     8461 aaaatagtcc tcggagaaga aggatgtcta agcgttcctt atatcgtagg cattgttcca
     8521 cgatatgaga aaattgttat tgagggacgc gatctaaata ataacaagat caagattaaa
     8581 gcatcaggaa cgctggcgcg tatttttcaa catgaaaacg accatcttga cggtattatt
     8641 tttattgata aagcaaaaga actttatcaa gtgaaatcag caaaaaatga ctaaaaatga
     8701 agtaaaaatt gtcttttggg gaacgcccta ctttgccgct cgtattctag aggggctaat
     8761 ttctgctcat ttttgcccta ttttagtggt tacagctcca gataaggtaa aaggccgagg
     8821 tttacaaaaa accccctcag aagtcaaaaa aatcgctaaa aaatggggtt tagaggtggt
     8881 tgaaccagcc aaattgcggc aaaatgaggc ttttttacaa caacttcgac aattagatcc
     8941 cgatcttttt attgtggcgg cttacggtaa aattattcca gctgaatatt taagcatccc
     9001 ccaaaaaggc gctttaaata ttcacccttc tcttttacca cgatggcgtg gcccctctcc
     9061 ccttcaagca accattctag cgggcgatac tgaaacaggt gtttctatta ttttaatgga
     9121 tgaagaaatg gatcacggac ccattatcaa aaactcaaaa ctcaactctc caattgcaaa
     9181 attaacttac agacaattag aagaaaaatg catcgaggaa gcaatcaaat tattaattga
     9241 aatattacct caatggttaa acggggaaat caaccccatt cctcaagatc acgagaaagc
     9301 aacttattgc aaaataatca aaaaagaaga tggactgaca gattttaatg agccagctga
     9361 attaattgaa agaaaaatcc gtgctttcga agtatggccc aatgtttatt tcgaacgcaa
     9421 cggaaaaaca tataagattt tggaggctga tatttttaaa gatgaaaata ttctcaaaaa
     9481 taaaacactt ggcgaatttt tctgtctgaa taaacaactc attgttaaat gcgctgataa
     9541 tggcttactt ataaaaaaaa tccaacccga aaacaaaaaa ccgcttgatg gctatgcttt
     9601 ttggtgcggc taccaaaaca aaatagcaaa ataaagaaaa gaaataaaaa atgaacaata
     9661 aaaaatggga agttataaaa cttcccattt aattttctta aattaaaaat taaaatttat
     9721 tgaaaataac ggcggcgcga gcataagctt cgcgcaaagc ttctgcggga atgtcttttt
     9781 tactcagatc aggattttca atgataatac tatctctagc ccaatatttt aatttgggct
     9841 gcttcttgcc atttttggtt ggaacaggaa caaaccacaa aacaaaactg cctatcctaa
     9901 aacgtatttc ctcaatttga ggtttcacct tgacctcctt tcataaattc ggcgataaca
     9961 tttctcacag agctgaccgg ctccttcaac ataatacttc cttaaattaa catgggtttc
    10021 ttttgagtac tccgtctcag caccacatga gacacaacaa tccttttttt ccctcttcct
    10081 attttccctc ttaaattttc taaacatttt tccctcctta aagaaaaatt tttaaattct
    10141 ctagttataa gaataatcaa aaaatattag tttgtcaaga aaaataataa aattttttcg
    10201 ttatttcaaa gataaaagca aaagaggcag ctgagcgaaa tttttaataa aaatttcgct
    10261 cagctacctc ggctttatat tttgtgctta atttaaagga ttttatcaat aaagaaaatt
    10321 actaatccaa aaactgtgga aatagcagaa ataatccaaa atcgcatggt aattttttct
    10381 tccggccaac caatggcctc aaaatgatgg tgaatgggtg aagaaagaaa aactttcttt
    10441 tttctaaatt ttttactgat cagttgcaca acgactgaaa gcgtttctac taaaggaata
    10501 aaagcaaaaa agggaagaag aaaagctgtg tttgtataca tcgctaaaac gcccaaagta
    10561 atccctaaag ccattgaacc tgtatcgccc ataaaaaaac gcgctggatg gatattgaac
    10621 cacaaaaaag ccaacagcgc tcccaaaata acgccaataa aagtagccaa atcaaaacga
    10681 ccttgaacaa aagaaactac ggccattgcc aaataagcaa aaagcataac accacccgct
    10741 agtccatctt ctccgtctgt taaattagta gcatgagctg tacctgcaac aacaaaaatg
    10801 aaaaataaaa tataccatgg ccccaaagaa atatctccca aaaatggaat atgtatcaca
    10861 tcccaattaa gtttaaaata gaaccaggaa gcgcctacca aagcaatgat aaaataaaga
    10921 attaattttt gcttaacact caaccccccg cccttcgggc ctctttttaa aacacctaaa
    10981 atatcatcgg ccaatccaat caaagctgcg ccaattaaag aaaaaagtgg taagtaagtt
    11041 tgggctcggc taacaaaatt aaaataatca aaaacctgat caaaaatttt acttaaaaga
    11101 aaaactatta aagcaataat ggcagtggta aaccatatta aaataccacc catagttggc
    11161 gttccttctt ttttctgatg aaaactagca aaaacaggcg ctccttctgc tctaatctgc
    11221 ttgccgacgt tatatttcga taaaattttt aacagaaccg gcgtgagtaa taacgctaaa
    11281 acaaacgaaa cggtcgttag aataaaaagg cgtgataatt gcaaaataat tagttcaaaa
    11341 gacataggct ttatttaacc agcgcaaaag tgtttctata tcattataac gatcgggcgt
    11401 ctttaaaaca actactaaaa ttcgacgcgg cgattcattg ctgcgctgac tcggaacaag
    11461 aaaaacaccg caaaacgttt gccaagcctc atcggtaaat cctgttttac cgccagcata
    11521 aacaacgttt aattcatcta aaaattttgg attgtgagcg attaaattga tattgaataa
    11581 attatgctct actgttgcgc ttttatcttg ttgttcataa accccttgaa ttttggtttc
    11641 ggcagttttg gaaatgatta ataaattagg atattcatta acaatatagc gcatcagttt
    11701 tttaagatca gaaggtgttg attgattgag aaaacttaaa ccagttggtt caacaaatgt
    11761 tgtctgagtc attcctaatt gctttgccat gtcattcatt aaacctacaa atcgatcctc
    11821 ccccatatgt aaagataagg ctttagcggc tttatttgaa gaaataatta aaagcgactg
    11881 gattaaatca ttaaccgtta gccgctcttg ttgataaaaa atatcgggat caccatcgat
    11941 atttaaagct tcttcagaaa ttaccacttc ttcattggat ggcaaatatt tttctgcaat
    12001 caaagcagtc attaattttg ttaatgaagc aatcggtctt cgcaaattgg gttgtttttg
    12061 ataaaatata aaatcagagt ctaaatcaac aaccagagcc ccctcggctt gaaccaaagg
    12121 gtcatcttgc gtatcccaat tgcgaaaagg ataagtaata gctgaaggat tagaacgaac
    12181 gctatattgt tctaaaaaca gacttccatt agtgcggaag ttttcattaa taaaaaaacg
    12241 ggcatacaac cagctaccgc caaaaccaat tccaacgccc accaaaacaa tgatcataat
    12301 tttaataatt tttttaaata cttccatgat tataaattat gatatttttc ataatcgggc
    12361 aaagcctcta ttttttctaa tcccattttt cttaaaaaat caaagctgac tttatacaca
    12421 aaggcgtttg ctcgatgtgg atcaatttct cgagtaatta aaccgcggat caataaattt
    12481 cttaaaatat aagaagaatt aacgcctcgg atattatcaa tttcggctcg actaattggc
    12541 cctttataag caataataga aagagtttct aaggcggcag gcgtaagatc ttcttttaaa
    12601 attttctgaa gaaatttttc aattgattct cttaaatcag gtgctgttgt taactgaact
    12661 tgttcatcaa aaataaccaa cgacaatccc cttcgattgt tttgatattc attttgcatt
    12721 acttgaacgg tttttttaat ttcttccaat ggaatctcta aagtatcggc taatttttga
    12781 aaagttaccg gttcaccggc aataaataaa atagcctcta tttttcgtgc taattcttca
    12841 ctgttcataa tgatgagatt gtttaatggt aattttaatt tcgccaaaat tttcctgctg
    12901 atcaataata attagttgtt ctttaaacat ttgcaaaacg gctaaaaaag taacaataac
    12961 ttctatcttt tctttgacgg atactaattg attaaattca gacgatgttc gttctgttaa
    13021 aattttaaga atgtttttaa ttttctcctc tatcttaata acttttttta ttttttcaat
    13081 tgttgtttcg cccaaactgc taaattcctg ccaaattttt acaaaagaat tgtaaagcat
    13141 ttgaggagtg cagttttcgg gcggataaaa aattggtgat tgattaacta aaaattctcg
    13201 agcaaacatt tgctgctgag taaaattttc ttttataaat tgagaggcat ttttaaataa
    13261 gcggtatatc tccaaacgtt cggttaaatc ttttatttct atttcttctt caggacttaa
    13321 ctctaatgtt ggcaacagac tctttgattt aattaaaatc agttttgcgg ctataactaa
    13381 aaagtccgct aaaatatcag gtgaagattg ctttaaatcg cctacataat ttaaaaaagc
    13441 atctgttacc tgcgcaagac taatttcagt aatttccatc tttctttctt caattaaact
    13501 caaaagcaca tcaagcggtc cactaaattt ctcttgctga atttctaatt gattaatttt
    13561 ttgctgattc ataatcttgc tttattttaa attctaaatt ataatccaaa tagtgcttat
    13621 taacatttta ttaaaaggag gtgtaaaatg gcaataaggt atggccgaaa aaattgtcat
    13681 catgaatggg ctataagagc agatggttac gaagaaggtt gcgcgccaat tatctgcatt
    13741 aaatgtgggg cttttggttg tttgtgtgat gcccgcaggg aatttaggaa gtggtatttt
    13801 ataaaaaaga tgggaattcc cgctagtctt tataatcgct tacaattttc tatgataata
    13861 ccagatgatt ataaagcaag acaaaaagaa cagaaattat tcgatctcat atttagccgg
    13921 aagtacaata gtactgataa tattaatcaa aagtggaaaa acccttacgt aaaaaaattg
    13981 aaaacagaaa agaacaaaaa ataaaataat ttattatgtc gcttcgttaa agacggagcg
    14041 acatttttat tttaacaaaa tttttatttg ttgtgaatgc ttagtattat tctacttgat
    14101 gtatttctac tggacgagcg atgaataata aaaggtttca actatttttt tcaattcaat
    14161 gcctgtaata attaaggaat aataatgcat aaataataaa ggtaaattaa aaaccgcatg
    14221 gttttacact gcggtttttt gtccctcata gcgctttttg atcttgtttc ctgctccgca
    14281 cgaatcctct gcaatttttt gaaccgcaat gacattcgaa cgactcttcc aggtgcgcaa
    14341 tcttatcata attaatcgta atttcctctc ctctcttaat atctctcagc gcttatccaa
    14401 ttcgttcttt ctcttcaata ttcggttcgc aagaatgatt gatataacat cctggtttct
    14461 cgctctttct aaattctata aattgattct cattatctcc catgacactt tttaaatttt
    14521 ttaccgctgc cacaagggca gggatcgttc cgaccaacat ttttaaattt ttcgggagaa
    14581 atatctgacg gcaatgaagg cgctggtttt gctacaggcg aaacttgctg tttttttatt
    14641 tgaaagataa tttcaaaaaa catttcttca aaacgatcaa agaaatcttt aaatagtcgg
    14701 aaagcattgg ttttatattc aaccagtgga tcatgctgac cataagccct taatctgact
    14761 gcctctctta aatattccag attttctaaa tgagctgtcc acaaattatc taaaacgttt
    14821 aacatcattg atttagaaat gttctctaac aactctattc caatttcagc tttaattctt
    14881 tcaaaagcag ttttgctttt ttccaaaaga aaattatata aactactttc ggcaatctca
    14941 ttgagcttaa cattttcttc aataatctta aactccttca aaaacgcttc aagattttca
    15001 atcggcacat taattaatct tgctatattt ttttcaataa tagactctac ttctgataaa
    15061 agatggtggg attgagctaa gtctaatatc ttgttgcgat aatcgtaaaa aactctccgt
    15121 tgtttattaa ggacatcatc aaattcaagg gtatactttc gagcgtcaaa atttaatcct
    15181 tcgattttcg tctgagctga ttcaatagcg cgattaacta tttttgattg aatgggcata
    15241 ccataaggat aattcaaaac gcccattaaa gatttaatac gttctccacc aaaaattctc
    15301 atcaaatcat cctccaaaga aacaaaaaat tgagaagagc caggatctcc ttgtctcccc
    15361 gctcgacccc gtaattgatc atctatccga cgcgcctcat gtctttctgt gccaatgaca
    15421 tgaagccctc ccaattcttt tactttagca acctcctcag caatcggcgg atttccacca
    15481 agaataatat ctactcccct tcccgccata ttagtagcta ctgtaacagc ttttagttta
    15541 cccgcttgag cgataatttc tccttctttt tcgtgatttt ttgcgttcaa aacattatga
    15601 ggaatgccct ctcgagaaag catcgctgat aatttttcgt tgttttcaat tgaaaccgta
    15661 ccaatcaaaa gcggttgacc tatgttatgt ctttttttaa tttcctcaac aatagcttcc
    15721 catttttctg tagaagtttt ataaactaaa tcaggcaaat ctttacgaat cattggttta
    15781 ttagttggaa taggaactac ttctaacccg tatactttgt caaattcttc agctgcagtt
    15841 aaagcagtgc ccgtcattcc tgctaatttc tcgtacattc gaaaataatt ttgaaaagtg
    15901 atgctagcaa gaatacgatt ttcttgtttt atttctacgc cttctttggc ctcaatggct
    15961 tgatgcaatc cgccggaata acgtcgtcca aaaagcaaac gaccggtgaa ttcatccaca
    16021 ataatcactt ctccatcttt aacaatataa tctttgtcac gcttgaatag agcgtaggct
    16081 ttaagagatt cttctagata atggacgtat cgaaaatttt ccggtgaata taaatttttc
    16141 actcctaaca ttttttctac tttttcaata ccactatcaa gaataagcac tgattttagt
    16201 ttttcatcaa cttgatagtc aatgtttttt tctaatcgag ggacgatttt tgcaaaaagc
    16261 ttatatgatt cactcgcctc catgtcagga atagtaatga ttagtggtgt tcgagcctca
    16321 tcaattaaaa tactatcaac ttcatcaaca attgcataat aatgacctcg ctgaactttt
    16381 tgatttaaat cataaactaa attatccctt aaataatcaa agccgaattc attattcgtg
    16441 ccataagtga tatcggcatc ataagctgtt ttacgcgaaa ctggccttaa atattcgtaa
    16501 aatactttaa aacttccagt tgcatctctt ttttcatcag tttcatcccc tttttcttta
    16561 ttattattct ggttgtttat cagaatattg gcagagttat tgacgttttt ataatcagga
    16621 tcataaagaa aagcttcatc atgggcaatg cagcctatag aaagaccgag taaataataa
    16681 acttgtccca tccaaactgt atcgcgttta gccaaataat cattcacggt aacaacatgt
    16741 actccttttt tagttaaagc attgagataa acaggtaaag ttgcggccag agtttttccc
    16801 tcgccggttt tcatttcggc aattttccct tggtgaagtg ccaaccctcc caacagctga
    16861 acatcataat gtctttgttt taaagcgcgt tttgccgctt ctcggaccaa ggcaaaaccc
    16921 tcaattaaaa cgttttctag gtctgcaggt gattttactt tttctctcaa actcagcgat
    16981 ttttctcgaa gagaaaaatc gctgagtgtt actaattcag actcaaaagc gtttacttta
    17041 tcaataaatt ttttattttg tttataaaat ttttggctaa gatctgaatg aaaaaaattc
    17101 aaacgcatat caatattatt acttatttat ttatattagc acatcttgag aaaaaatcaa
    17161 aaaaataccc acgtataatt tctttctttg attttaatat agattattta taatggatgt
    17221 tatggtaaaa ataaataata actatttttt attgcgccat ggcgaagcat tttcaaacag
    17281 agaaccgcgc tggctttctt cctggccaga aattaaaata gcccatctga catcgcgggg
    17341 taaattaaaa attaaaaaac aagctcaaat ttttaaaact aaaggcatag acctgatttt
    17401 ttcctctgat ttagctcgaa ccaaagaaac agcccaaatt gttgctcaaa ttattaaaaa
    17461 gcctgtcatt tttaaaaaag aactgcgaga attaaacttc ggcgtcttta acggatttca
    17521 tcccaatgtc tattgggctt attttcaaaa tcctctagaa cgttttacca aaaagattcc
    17581 acatggcgaa acactcaatc aatgcgccaa aagaattttc aatttttgga aaaaaataaa
    17641 ttgccgctat caaaacaaaa acattttaat tattagccat gccgatccgc tttggctact
    17701 agagtcaaaa attagaaagt taacaaaaat tcaaatggta aaaaattatc gattacgctt
    17761 aagtcccggc gaagtaagga ttatcagagc gcattctaaa gattaaccat taccatagct
    17821 acaatagcca aaaataaaaa aatattgatc aaaatattac ttgctaaaat aaattgggct
    17881 aattttttga tgcgaaacca gaagataaaa gccaaaagaa gatgaagggc tgaaaagaag
    17941 aaaccaacaa accaaaaaag ataaaaattc caaaagtaac cggtcatttt aatgccttcg
    18001 agcgaagtat aataaagaat ataagaacgc tgaggcaaaa atttgatatt aagaaaaata
    18061 ataaccaaag ctaccaataa tataataata ttaatgatta gagaaattct aacccaacga
    18121 tcatgtaaaa aaatttttag ttcgtcctta gtcatagaaa caattataaa aatttcatct
    18181 ttaaagtcaa aaacaggtat ctacttgcag tttgtgctaa aaaaattcat gactttttca
    18241 attttacagt agcaatcctt ggcgcccctg atgggaatcg gacccatgtt tccaccttga
    18301 gagagtggcg tcctagccac tagacgacag gggcttaatt gtttatttta actaaaattg
    18361 agaaaaaata aagaaatttg ctagttttct aattgacaaa cattctaaaa aagattaagc
    18421 taaaattagc tatttaattt taaaagaaag gaggattaag atgaaccgaa aagttcgttt
    18481 tcttaaatgg acccctcaaa caaaaattga tgctgttatt gcgctaggaa tgggcttcaa
    18541 aagagacttc accgatattt ctgatcaaac aaaaagtatc tgttctaccg ctgctttaat
    18601 ttctatccaa aataatattc cactagtttt agtgggacaa gaaatggatg atgaacaaga
    18661 ggaaacagca aaatgtgttc ctaatgaaag tgtcaaaatg taccgttttg tcagatatct
    18721 gcacactgaa caaaatatcg tagaaaaaat ttctcaatta aatgctcgat ctctttatgg
    18781 gaaactcgat acagttctag aattagttga aaaaacggga tgggaaaaaa ttgccattat
    18841 ctgtcatgtt gacatgatgc caagaataaa aaaagttttg aaacaattag gcttgatgaa
    18901 agaagttgaa gtttctgttt atctcgctcc cattcggccc atgtacaatg gcgattctga
    18961 tttctggtgg ctacgcgaaa attggcgtgc aaagctctac gaaatgagca gtagtttatt
    19021 caatttgatt aatcgttaaa ataatttaag gtcgctcctg cagcgacctt tttatttttt
    19081 taattcttca acaatttttt ctagttgcat cgcgtgtgat cctttaatta aaattgcatc
    19141 attactgcta atttcttttt taattatttt actggcgctt tcgctattat taaagacaag
    19201 cactttattt ttatcaaaac cgtgttttat tgcagattcg gcaatcaacc gcgccgctga
    19261 accaacgcaa atcagaaaat caactttttt aattacttct ttccctattt cttcgtgtgc
    19321 ctcagttgta tatttgccta attctaacat atcgcctaaa atagccaccc tgcggcgttg
    19381 agtgaaatgg cctagtgttt ccagagcagc tttcattgag aggggtgatg cattatagca
    19441 atcgtctaaa ataacgctat ttttaatacc ttttataatc tgcattcgat gatcgggtgg
    19501 ttgataatta gctagataat cgctaatttt aacaagattg ataccaaaaa caattcctac
    19561 cgctacggct gctgctatgg aataaatctg aggcttgccc ataacccgaa aaagacgaaa
    19621 aggaacaaaa acattaccat actgaatttt aaaagtgata ccttcgggaa attcattttc
    19681 atcaaattta tattcaaaat taataatttg aatatcagca tcctctttaa acccataact
    19741 aattatgtgg gcgcgtgttt ttattctcaa attccacacc aattcatcat cacgatttaa
    19801 aatagcaaaa ccgttagcgg gtagcaattc gattaatcgc gctttttcta ctgccaccgc
    19861 agtaggacta gaataaaact ccacatgaac cggtatttct cctatactcg tgatgactcc
    19921 aattttaggt cgggctaaac gtgctaaata ttgtatatcc cctggtcgat ccgcagccat
    19981 ttctaaaact aaaatttcgg gatatttttt attaaaaata agaagttggc caatagcttg
    20041 aaaaattact tttggccaaa acaaaattcc gcgaattgca ttataatcac ccaaaattgt
    20101 tagtggcact cctaattcat tattaaaatt cccttgcgag cgtcttacat tccgttctcc
    20161 tttcaaaaca gtatagacag cttctttggt agaagttttg ccaacactac ctgtaatgcc
    20221 aatcactgcc ggtttatatt tcttcaatgt ccattgagcg agaattcgca aaatattttt
    20281 taaagtatta aatagcaggg gggatttcat agtagtctaa aataaattga gcgagctctt
    20341 taaacattgg aactacggtc gaccccgcca aagatgcttc tggtttgtct aatttaacaa
    20401 gaataataaa acgcgggtca tcagctggag cgtaaccaat aaaagaatgg atggttttat
    20461 ctgaataagt gccatttgca tctggcactt gagctgtgcc agtttttcca gcaatttgat
    20521 aaccttgaat ttcagccact ttattttttt caacagaatt aaccataata attcttaatt
    20581 gatccgctgt tcctttacta atcacttgtt ttttaggttt tggttcaaat gtcttttctt
    20641 tatttccttc aggatcaaca attgtttcta ctacataagg agtcatcatt aacccattat
    20701 tggcgatagt tgaaaaagca ttaatcatcg cgataggcgt aacagaaatg ccctgaccaa
    20761 atgaggcggt agcaaaataa atatcgttaa aacttttcaa attattaata ttaccaaccg
    20821 cttcattagg caaatcaatg cctgtttttt gattaaatcc gaagtttaag acatgttgat
    20881 gaaaaatatc atgaccgatt aatttttcta aataaacggc gcccgtattc aagctataag
    20941 caataatatc aaaaacattg cactcgccgt gcgctttaaa atcccagttt ttgattgttt
    21001 ttccactaac aactacttcg ccattatcat aaaattttgt ttgaggagta atccgttgcg
    21061 aatcaagtcc agccgctact gtaataattt taaacactga tcctggttcg taggttaatt
    21121 gaatggctga atttaaaaaa gagttcaacg aataggatga atatcgatta ggatcgaaat
    21181 taggataatt ggctacggtt aaaacttttc ctgtctcagg ttccataacg ataattgatc
    21241 caccctcagc tttccattct tttgcctttt tttctaataa atcctccgcc ttattttgaa
    21301 tatgaaaatc tattgttgta acaatatcat tattggctcg actataaatt aaatttctca
    21361 atgataaaaa atcaaataaa tcattagata aagattggtt ggacaaataa tcatcgtagt
    21421 atttttctaa accatattga ccatgaaaat tcttaacatc atccttaaca taaccaatca
    21481 attgagaagc aaattcttta tatggataat aacgaccaat atattcctta gtgtaaattc
    21541 ctttaagatt aagatcttct attttttgcg cggttgtttc atcaactttt ttagttaata
    21601 attcatagag actttgcttt ttggaaaatt taataatcaa atcttcatat gataaattga
    21661 gctgcttaga taaaacgcta gctgctgcta ctggatcatc aatttctttg ggcactgcat
    21721 agatctcaaa aaatttttta ttaaccgcga cgggaattaa tgacccctct cgcgtttgaa
    21781 aataaattgt tcctcttaaa tattgaagat tgtttttaat ttgttcggca gtctttactt
    21841 gagcagccgc cttttgcgaa aaatcttcat aatgaagaat ttgcaatgaa taaagccgcc
    21901 ataaaattat gccagcgctt aagacaaaaa ttattaaaat aaaatgcaaa cgccaattat
    21961 ttaaaaattg tttcgctttt tttcgcttca tctcaataat tcattataaa ttattcaaat
    22021 tggttaaacc aacctccatt tgtggatttt taaccaaaga aaaatatcca ggaacagcta
    22081 ctagaattaa accctctttt tcggcgatcg ctgtaaattt atcaaaactt tgaagagagg
    22141 ctacctgatt ttttaattca gcatttttct gttcaaaaac aactgattgg gaagttaagt
    22201 ttttaatatc aaagcgataa ttaaccattt taatctctaa aaataagctc ccaacgatag
    22261 taaaaataat taaaatcatc aaaccaatat tcaaaaacgc aacaaaagaa aaacgagaat
    22321 ttaaaacttt actatttttc ttttgccaat tcattcgttg cgtaactaaa ttatagcgat
    22381 tatgttttaa actaataatt gtcatgattg ttttattttt tgagccaccc gcaatttggc
    22441 ggaacggcag cgacgattat ttaaaatttc gaccttggta ggagtaactg gttttttagt
    22501 aattattttc aaaaccccct ccttttgttt atccctaaaa aaatttttaa caattcgatc
    22561 ctctaatgaa tgatagctga caacagcaat tcttccttta acatctaaaa tttttaccga
    22621 tcctttaagt gcttcagtta aattgtttaa ttcatcattt acataaattc gtaaagcttg
    22681 gaaaattttt gttgctggat gaatttttgt ttttcttctt cccgtctttg tcacaatctc
    22741 cactaattgc gatgttgtcg tgatacgtcg tttctttcgt tcttccacaa tagttttagc
    22801 aatgcgataa gcattttttt cttctccgta ctcacgaaaa attttggtca attcattaac
    22861 actcatttta ttaataacct cctcagcggt taaactaccc tgggaatcaa aagtcatttt
    22921 taacggttcg tcccgcaaaa aagaaaatcc tctctgcgat tcttcaattt gaaaactacc
    22981 taatcctaaa tcgaagacaa tgccattaac cggtttaaat ttttctttct gagcaattcg
    23041 agcaatattt ttaaaatttc cttgccgcaa gatcacgcga tccttaaaag cgactaatcg
    23101 tctctcggca acttttaaca cttcactatc taaatcaatt cctaaaactt ttcccaacgg
    23161 cgccgtatgt tgaagaattg ctaaggtgta tcccccttca cccaacgttg catcaataaa
    23221 attttcattc ggctgaggat caacaaattg aatgacttct tgtaaaagag ctgggatgtg
    23281 catattttaa atcccgaagt taattaattt ctcggcaata tcattagcat ctctttcggt
    23341 ttttattcga tattcattcc aaagattttc atcccaaatt tccaaacgat tgcttacgcc
    23401 agcaataacc acttttttat ttaatccggc aaattttctg agataatcgg gaataagaac
    23461 tctcccctgt tgatcaatct cagactccac cgctccggct aacataaatc tggaaaaggc
    23521 gcgcgaattt gattgcgtta atggcaaatt aaccaattga ctcagcaata cctcccattc
    23581 ttttttagga taaacaaaca agcaagaatc aagaccgcgt gtaataatca ccgtctcacc
    23641 taattcatga cgtaatttgg ccggcaaagc caatcttccc ttggtgtcta aattgtaatg
    23701 atattcgcca atgagcatat tacttatcca caattaagat tatttatccc cacagttatc
    23761 cacaattccc cactacaaat atataataca caatttcgaa aacaattgca aaataagaac
    23821 aaacgagtag gaattgcttt ttaaattaac taatagctaa aaaaaccgcg ttttaagcgg
    23881 tttttttaag aaatagcgga ttactctttt aaattttttc cacaacttat ccacactgaa
    23941 ttcgtctact ctaaaaatcc cccttttgta gaaattccca ctcttcaggt ggaagttttg
    24001 gcaattcacg aatgggagtt tttccaggat aacgccaaac gctgataatg cgaatttttt
    24061 gatgagataa acccgctgaa atttttttgt cttttttgtt ttgttttgtt ttaatttctt
    24121 gcaacataac ccaaatttct ttagggtgtt ttatagtgcc agcattttgc ataacggcaa
    24181 tagtctgagg agcaatcccc tcttctatac gatggggtgt gtgtaacact ctcttaattc
    24241 gtgattccga taattgatac tgcatcattt tttcaattga atgttttgtc caaacaaccg
    24301 tttctgaatt ttgaggaatt ttaaagcgca tattttattt agaaattttt aatttgtaaa
    24361 aaattctatt cttctcacta ctttgcaatc tgcttttcga aacaacatca gcaaaaaatt
    24421 tgatactttg atcatacgtg atgcgatcta cagggtaagg cgtagcgtct ttgccgccat
    24481 gcgcaaagga ataacgtgct ggatctcgat aagatggctt agctccataa ataatttcag
    24541 aaaccaaaga caaagctcga atagtttttg gacctactcc tttaagagat aaaagttttt
    24601 catatgatgc tggccgttgt tcacaaacaa gatataaaat tttttgtaaa tatttattct
    24661 tgaaaaagtt ttcttttaca actggatgcc aaacaaattc tcgattttct aaattaagaa
    24721 atatagtttg ttgtccatct gatttcactt ttaatacttg ggagtaatca gaaaaatgtt
    24781 ttgctaaaat tttaatatct ttcattaaat cataataatt attttgtact aattgagtgc
    24841 ttactgatcg agttttttca ctttttaatg ctgttaaatt tagcgttggc tgatttaaaa
    24901 tttgacttgc aattcctgca tgaggttctt taactaaatc tgttatcttt ttagaatacc
    24961 aatgataacg tcgcgccgac tgattctgaa tattcattcc ttgttggatt actgtccaac
    25021 tgccattttt cgaaaaaaac aaagtgtgat gatagatctg aaatccatct tgaaccaaag
    25081 cgctatcaac ctttgctgtc attttagaat tataaattaa agaattaaca taaccatccg
    25141 gtaagctcaa agcattaccc cattgttcaa tttcttcagg ggttctgcgt gaagtttttc
    25201 ccttacctcc gcaaataaaa attcctagtt ctttttctcg tcctcgaata gcttctttta
    25261 gggcggctgt caaaatggta gttaaacccg acgaattcca atcaaaagct aaaaccgtcc
    25321 ccagcgattg aaaccagaca ggatcagaaa ttctcttaat aaattcatcg gatccgtact
    25381 cattaataat cacttctata atttctcgac ctaattttgt cattcgctca aaaagccaac
    25441 gcggacactt cccttcatct aagccaaaag ttgcaattcc tcgataaatc atacttcatt
    25501 attatagcaa tttttgaagg taaaataaat tgatttttgt tttgaattta gtaaaatatt
    25561 gttaaattgg gcggttagct cagctggttt agagcgtacg gttcacatcc gtaaggtcag
    25621 aggttcgaat cctctaccgc ccacttctta aaaacaaccc gctaaagggt tgtttttaat
    25681 gagtaaactc ttttttctta tttatttaat ttccactgta gcgcctgctt cttccaatgt
    25741 ctttt
//
LOCUS       DAOSMC010000006        25096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_2509_length_25096_cov_18.838066, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000006 DAOSMC010000000
VERSION     DAOSMC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 25096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25096
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2509_length_25096_cov_18.838066"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PL075_01200"
     CDS             <1..252
                     /locus_tag="PL075_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96847.1"
                     /translation="CGICCSSCGLYVREICTGCNKSKEGVEFLKKINANCPVLECAVE
                     NKVDVCSRDCEKFPCDQFQNWPLSEEWIKMYKERLNNSE"
     gene            342..872
                     /locus_tag="PL075_01205"
     CDS             342..872
                     /locus_tag="PL075_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOD96848.1"
                     /translation="MVKIPVIKSKNFILRPFNKEDEKSLQGNINNKKIYQNTSNIPHP
                     YTLKDAKKWITKNLKEEKKKNPQMINFAIEINDEVVGSVGLGNIKYGHKAEVSYWLGE
                     KYWNRGIMTEAVKLVTKFGFEKLKLKKIYGRVYIFNLASKKVLEKNDYKLEGILKKDF
                     KKDGKFIDSYLLAKIK"
     gene            complement(996..1066)
                     /locus_tag="PL075_01210"
     tRNA            complement(996..1066)
                     /locus_tag="PL075_01210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Gln,seq:ttg)
     gene            complement(1305..1529)
                     /locus_tag="PL075_01215"
     CDS             complement(1305..1529)
                     /locus_tag="PL075_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96849.1"
                     /translation="MLKKDYLYGYYVQYDDEVAYDGFVYLRDRLDQNEVQVFFNEASR
                     RGRADFEDDYGRNFVLIKNTDGTFFISRRD"
     gene            complement(1561..2664)
                     /gene="dnaX"
                     /locus_tag="PL075_01220"
     CDS             complement(1561..2664)
                     /gene="dnaX"
                     /locus_tag="PL075_01220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOD96850.1"
                     /translation="MSYLALYRRYRPKTFAEVIGQKSIIQTLCNALATNHVSHAYLFC
                     GQRGTGKTTVARLLAKAVNCENRKENEHEPCNQCGSCIEINEGRSLDLIEIDAASNRG
                     IDEIRELKDGIRFAPVKSKYKVFIIDEVHMLTKEAFNALLKTLEEPPAHALFILATTE
                     PEKLPPTIISRTQRFDFKKLTLNEIISRLKILAEAEKINITPDALKEIAVSAEGSLRD
                     AESFLDQIISLGYKQIDLDSLEEVLGRVSFSKIIQFLDYLLQKDTKNAIIFISNLYEN
                     GVDLINFSRAVLKLARQIMLIKINPQINQIIQDEFTEDQLEKVNQLAQSFPMNDLQKL
                     IDSLLKIKEQIKYSPIQTLPLEMIVIQLCSENK"
     gene            2910..4136
                     /locus_tag="PL075_01225"
     CDS             2910..4136
                     /locus_tag="PL075_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOD96851.1"
                     /translation="MKRKISTPLMITFVVVILVIVVVVISQFFNTVHFYFNLAGQSQA
                     IVVDNSPKRLENPPKDIKAIYLTAYTASNDKRMTEIIDLVNKTELNAVVIDLKDYSGH
                     ILFDTQSDFLKKFGTEKVLIKDLEGLINKLHQNGIYTIGRITVFQDPILAKARPDLAV
                     KNKQTNDLWKDNGGLAWLDPAAPEIWNYIFVLSKSAMRYGFDELNFDYIRFPSDGILS
                     NMAFPVYDGQVEKARVLKDFSEYLSANLRPWGIKISADVFGLTTVFNHDMNIGQVFEN
                     FLPYFDYICPMVYPSHYESGFLGYENPAAHPYEVIKYSLDKAQERIDQFKEKQTVGDT
                     SQNIKVAVLRPWLQNFDLGATYNASMIIKEKEAVYDSLCPNWKTEGFDNCSNYSGWFL
                     WDPRNIYTPSALENEF"
     gene            4147..4854
                     /gene="rsmI"
                     /locus_tag="PL075_01230"
     CDS             4147..4854
                     /gene="rsmI"
                     /locus_tag="PL075_01230"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOD96852.1"
                     /translation="MKLYIIATPIGNLEDITLRALKILKEVDFILCEDTKHSLRLLNH
                     YQINKPLISYHQHSRLNKVNQIIDLLKQGKNLALISDAGTPGISDPGNELIKMILKEI
                     HEVEIVPVPGASVVSALCSISGFNTNRFLFLGFPPMKKQRQKFLKEIADSPYPVVFYE
                     SPYRIIKTLSELNQYDKGSRLEVVVGKELTKMFEKIYRGKISEVLTAIQKDGVKGEYC
                     VIVSCEDSKSNLTKNKK"
     gene            4904..5113
                     /locus_tag="PL075_01235"
     CDS             4904..5113
                     /locus_tag="PL075_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96853.1"
                     /translation="MFFICAIERRKKEKTKDFLVRVFKFKKMVEKELRCKLKIVVYVD
                     DQQISWAKILNTRQKHPNPNLLKQK"
     gene            5183..5482
                     /locus_tag="PL075_01240"
     CDS             5183..5482
                     /locus_tag="PL075_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96854.1"
                     /translation="MSFEQPNNFNKIEYDPEMEKQERWRGEIEAEVGKEISEGQYEKV
                     EVQMLKEEVKHQSKDMSDEEAYRIALEIWKQIEVDKRFNQKVQEEKEREEKIIDK"
     gene            5964..6413
                     /locus_tag="PL075_01245"
     CDS             5964..6413
                     /locus_tag="PL075_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96855.1"
                     /translation="MEESFVYAGGGISGLTYEECVGWRKYVASKMPSYIHILSPMRGK
                     EYLNNGKIISGSYEEHPLCSQKGILCRDHYDVRRANLVLMNFLGLQVVSIGSIVEIGW
                     TYAYRHPLITLMEKNNVHDHPMIREISNFILTDLDEAIELIITILTP"
     gene            complement(6458..6531)
                     /locus_tag="PL075_01250"
     tRNA            complement(6458..6531)
                     /locus_tag="PL075_01250"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6495..6497),aa:Ala,seq:tgc)
     gene            6612..8486
                     /gene="argS"
                     /locus_tag="PL075_01255"
     CDS             6612..8486
                     /gene="argS"
                     /locus_tag="PL075_01255"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOD96856.1"
                     /translation="MSDQKDKLQKFIKKYYQKEEKNIEQIKQRIEKEKEGRQFIDRAI
                     YYLFQIQEEVREIIDKNYSGIVFDEKDVILEEPPLFIKSDLCFSCFSMAKKLKKSPVL
                     LSEEIVNLINASTEKEFIAEATSEGPYINLNLKSEIYSKVLAQIINLGEQYGESNLNS
                     GEIILIDYSSPNIAKPIGLNHLPSTIIGHVLGNLYYATGASVIRHNYLGDWGTNFGAL
                     LWAYFHWGDDKKIQENPVIELKNLYVRFVEEASQNETMINEARELFNQLENGDEKLLR
                     IWKKFRDLSIKDFKKVYRRLGITFDCYLGESYFLDQTDKVINEAIEKGVAKENSDNKL
                     IVVDSFEGLPSFLLRKEDGSTLYLSRDLAALKVRFELFNLDWLLYVVGSEQSLHFQQL
                     FALAEAVGYLNEKKKAKHIPFGLILIEGKKMATRKGSLIEMDELINEAIKRAEDLITK
                     KNSEMSESESKKIAEQVGLGAVIYNSLKQSRLQNINFDWEKVLNFESASAPYLQYSCV
                     RIKSILRRIDLKGDIKISGDQLTKAVFENPTEFEILKKLMLFPMVIIKAQKEDAPHLL
                     CTYLEELAQLFNKFYDNVSVIKTTDESLKNSRVFLIKATLQILEKGLALLNIRVPNKM
                     "
     gene            complement(8555..9463)
                     /locus_tag="PL075_01260"
     CDS             complement(8555..9463)
                     /locus_tag="PL075_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96857.1"
                     /translation="MNKKIALIISLSLFITVPLMAATTNSQSANQTGNKPESTSAAPA
                     LSPQITEKPVLPINAPGLQKNIQEKNQIQTQNQGGETNIQQQTEEQNQVTTTTPAPKT
                     IVPRPNKTLNPRSETAREHMSIVAQKVEELLTATTTEITGIGQQVREIARNQKEIQQQ
                     IQERIEKIDSRPKWLKSIFGPDLTSINQVKKHLDQIQLRIQQLEQLKNQLTNAGEQQM
                     VQELINALTQQQIVLNDYLTNEEQYSSLWGKIVRLFSRKPEASPSPTPTASPTLTPTP
                     TETTTPTPTPTLTPTATPTITPTPTL"
     gene            complement(9525..9595)
                     /locus_tag="PL075_01265"
     tRNA            complement(9525..9595)
                     /locus_tag="PL075_01265"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9561..9563),aa:Pro,seq:ggg)
     gene            9992..10612
                     /locus_tag="PL075_01270"
     CDS             9992..10612
                     /locus_tag="PL075_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOD96858.1"
                     /translation="MSGSILIFQLIILLFSVILHEVSHGYTAYILGDDTAKVNGRLTL
                     NPLPHLDLWGSFLFPMISYLISGGTFIFGWAKPVPFNPLKLKKPKRDIGLVGLAGPLA
                     NLFVALCFSIIIRLAHIFNWAFLTPLFTFFSIIIYLNILLAIFNLVPIPPLDGSRVIY
                     SLLPRKWEKFYWSLERFGFILVMIFVLFGFQLIIPIIDSLFNLFVG"
     gene            10749..12875
                     /locus_tag="PL075_01275"
     CDS             10749..12875
                     /locus_tag="PL075_01275"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOD96859.1"
                     /translation="MKNKILKVQKRDGSIIDFDETRIHDAIYKAITATDQGDGKRTKK
                     LTEKVIDLLNRRFKADEIPHVEQIQDIVEEVLILEGLTETAKAYILYREQRRRIRESV
                     ALVDESIEMVDKYISEIDWQVHENANMTYSLQGLNQYIIAQVEKKYWLNKIYPKEIRE
                     AAQNEDFHIHNLETLAPYCMGWDLMDLLLKGFGGVPGKVESLPPKHFRTALGQLVNFL
                     FTLQGETAGANAVSNLDTLLAPFIRYDHLSQREVKQALQEFLFNCMVPTRVGFQTPFL
                     NVSLDIKPPQFLASQSVIIGGQPQKETYGDFQEEMDLFNKTFYEVMLEGDAKGRPFTF
                     PIPTVSITKDFDWDNPALIPMWEATAKYGINYFSNFVNSDMKPEDFRSMCCRLRLDNR
                     ELYKRGGGLFGAAPLTGSIGVCTINMPRIGYLSKTKKEYFERLAHLMDLAKESLEIKR
                     KTLENFIEKGLYPYTKFYLSSVKNIRGQYFGNHFSTIGLMGMNESLLNFLGEDISTKR
                     GIKFTLEVLDFMRDRLVDYQKETGNLYNLEATPGEGTSYRQAKVDKEKYPDIITSGTK
                     EVPYYTNSTMLPVNFTDDIFESLKLQNDIQTKYTGGIVTHIFLGERLSDITTTKNLIR
                     TVCSKFNIPYITLTPTFSICPTHGYINGEHFFCPKCAVKQPCEVYSRIVGYLRPVSQW
                     NFGKQQEFRDRKEFNVKKIKLPVSKK"
     gene            12885..13586
                     /locus_tag="PL075_01280"
     CDS             12885..13586
                     /locus_tag="PL075_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOD96860.1"
                     /translation="MEIGGLQKTTLIDYPGKIACTIFLIGCNFRCPFCYSAELVLPEK
                     IKNQPRIDKKELFRFLKERQKYLEGVVICGGEPTIHSDLSDFLLQIKKMGYLIKLDTN
                     GTQPDVIKKLIDKKLVDYIAMDIKAPLEEEKYAAASGVKVDLEKIRQSIALIKNSGID
                     YEFRTTVVPTIHQLEDILNIAQAIAPAQKFYLQNFRPEKTLNENLLQVKPFSETDLEM
                     ILEKIKNLFEECKIR"
     gene            13754..14485
                     /locus_tag="PL075_01285"
     CDS             13754..14485
                     /locus_tag="PL075_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96861.1"
                     /translation="MLNERQKKIIDLIIKEYSKNQKPVGSRYFCRKYFPDLSPATIRR
                     EFHILTDQKYLFQPHCSAGRLPTDKAYDWFVQENLINRPSQKEELDHWQKKLVDLKNS
                     SFSETAKRIAALCDSLTIGYLPWEQLIYKYGFKNLFARLVEINLNEWKTIWEDIDQLD
                     DRLEKIANHLMEEELQIFLGEDSPLTRDRNLSVISSVIPYNRRKTLLILIGPKNMICE
                     KNLNILEAARNILNQINNQQYGRTK"
     gene            14469..15005
                     /locus_tag="PL075_01290"
     CDS             14469..15005
                     /locus_tag="PL075_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOD96862.1"
                     /translation="MAEQNKNNQMDAEEKEIKEKLQDLTQKLEECEKLKEEYLNGWQR
                     ERAAFLNYQKDTSKRIEEIIKMANEGLIRELLSVLDSFDISVNSLNVEGLTPTEKNIV
                     RGIELIRGQLLDVLKQQGLKEIQAIGSQFDPHLHEAIDIVKGGKDGEVAEELIKGYTL
                     NDKVIRPAKVKVFKEDKK"
     gene            15060..16943
                     /gene="dnaK"
                     /locus_tag="PL075_01295"
     CDS             15060..16943
                     /gene="dnaK"
                     /locus_tag="PL075_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOD96863.1"
                     /translation="MSKILGIDLGTTNSAMAIIEGGQPKIIENKEGMRTTPSVVAIAK
                     NGERLVGVLAKRQSVTNAQNTIFSIKRLIGRKYTDSEVQRDKKWLPYEIRESASGGVE
                     VKMGDQWYRPEEISAMILQKLKTDAEEKLGEKIEEAIITVPAYFDDSQRQATKNAGEI
                     AGFKVRRILNEPTAAALAYGLNKKTNERIVVYDFGGGTFDISILQVSEDTVEVISTGG
                     DTHLGGDDFDQRIINYFVEEFKKREGIDLSKDLLAVQRLKEAAERAKHELSTVLETDV
                     NLPFITSDASGPKHFEMKLTRAKLEELVKDYIDRSVELVKQTLAEKNLKTSDIDEVIL
                     VGGQTRMPAIQEAVKKFFGKEPHKGINPDEVVAMGAAVEGGILQGEVRDVLLLDVTPL
                     SLGIETLGGVFTKIIEKNTTIPVSKSQIFSTAADNQTSVEIHVLQGEREMAADNKTLA
                     RFILDGIPPAPRGVPQIEVTFDIDANGILKVTAKDKATGKEQSVRIEASTGLTKEEIE
                     KMKREAEQHLSEDKRKRELVETKNQAENTIYLAEKTLREAGEKITPEDRKEVEDKIND
                     LKQVLGGDDLENIKKRLEDLSLAISKIGAKMYQGGQQTNNPNNPDNSSSDDNKPEDAE
                     FKD"
     gene            16955..18007
                     /locus_tag="PL075_01300"
     CDS             16955..18007
                     /locus_tag="PL075_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96864.1"
                     /translation="MAILEIFSSTNHSTEKSNYWWKILIFTVLALITASLAGASLGYL
                     FLGKSFLGFSSFWGFLIFLCLFGLFFIMESLFAQGARFGAVLLESFIFALSFYAIRIG
                     FSKFNGFMLMWSLGVWLLFLLCLWGGRFKMRQRKSDLLRLKWNEMVKGGLALVLIGVN
                     LFICLQWMGNVLIQPDRLFTQKTVDMILKPATPLLQVYFTDFDPYMTVDAFTKSVISE
                     QVESMFVQSEDSKNYIPKNIIEQQKKDLIEQNSFAFKSQLGELLGFPLSGTEQINTVV
                     FQFLWMKFQSLSIATKQYIILGISFLMFLVFRILAIFIGWVIQMIGWFIYELLLAGGF
                     GAVKIEMKNKEDLVLF"
     gene            18021..19154
                     /gene="dnaJ"
                     /locus_tag="PL075_01305"
     CDS             18021..19154
                     /gene="dnaJ"
                     /locus_tag="PL075_01305"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOD96865.1"
                     /translation="MEKKDYYKILGVNRNASQEEIKKAFYKLAHQYHPDKAGGNEEKF
                     KEINEAYQVLSDENKRRQYDQFGQTFEGAGASPNWGFDFSNFGQGGFNNSFGSKVDFD
                     FSDLGLDEDLGDIFNNIFGGFNTGQRKRKKVQYGSDVRLMIEISLEEVATGVVKEIEY
                     NTFVKCDHCQGRGYEEGSGFKTCPTCNGRGEIKEIRQSFFGSLSQVKICPECGGEGKI
                     PEKICSFCKGHGRVKGKKRISLKIEPGINDGQIIRVAGGGEDGEKNGPSGDLYVEVRV
                     KPHSLFKREGADLIYQTSIPVLEAILGTEKEIPTIYGKPIILKIPAGIDSGKILRVRN
                     KGLPYFGRRGYGDLLVHIKVKIPKKVSSKAKKILEDLRGELND"
     gene            19159..20175
                     /locus_tag="PL075_01310"
     CDS             19159..20175
                     /locus_tag="PL075_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOD96866.1"
                     /translation="MNIALTYNLRRVKKLSKNKKVIQNIDFDDPKTIQGIKKSLECLG
                     HKVFLVEADENAYFKFKRLQKRIDLVFNYSEGLHGKDREAQIPAMLEMLRIPYTGGSP
                     LAYAIGLNKTKTKEILSYHRIATPKWQVFKTGREKLDRHLKFPLIVKPQSEGSSKGIF
                     IKSLVKNEKELRKIVKEEINDYHQPIIAEEFLSGREFTVGVLGCPPKVLPIIEVRFDE
                     LPKGIPKFDHFEAKWLYDNPKFKADPLLCPAPITAKLKKEIEDMVLKAFEVLEMADWA
                     RFDLRLDQQGRPNILEVNCPAGLNPDPKENSRFPRAARVAGLSFEELLQEIINSAVKR
                     QEIK"
     gene            20261..20509
                     /locus_tag="PL075_01315"
     CDS             20261..20509
                     /locus_tag="PL075_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96867.1"
                     /translation="MKLIKRFLKPTGEAVILIKPQFEVGRGNLNKRGVVKDLSRSQQA
                     VKEILRIVQREGHRIKGPIASSLKGRAGNQEFILYLKM"
     gene            20672..22003
                     /locus_tag="PL075_01320"
     CDS             20672..22003
                     /locus_tag="PL075_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96868.1"
                     /translation="MSTPEVKKTLSVESKEASASSSKTGTTPVFRIDEILTKAGRSLF
                     DKISDSGKAVVHGLYEGAYKSPGINRLVGKMEIAYNQFRGDKYEKKAAKFKNKLDGVD
                     IKINAFQQAKENLAGVIKDLKQQNIPAATALELKIKDIEKQINKLADKRDRIQTKFEE
                     KESRRNLRINKRDQIANRLIEYYENKLEPFEKKLEELRKEQDHIDLLMMATEVKHKNR
                     EVALDKLQKKLDFTIENYKRLGMSERKIRKDEAVKAFNEQIANARKEMQLENKKLQLA
                     KDKLDTKIAKVDAKANPYRDRREEFVRVTKVRPIEFNVPGREKGQPHEGKEEIKTHER
                     KESEVKKTESASKVEGKIETAFEEEEEKEKFPVAAYLATLNDYLLKNYDKTYVINKKD
                     YYAQTQLSRYDKMEVADFRRILLQYAKFKRMPTQKVDIYEKAINNLFEKNK"
     gene            22028..22336
                     /locus_tag="PL075_01325"
     CDS             22028..22336
                     /locus_tag="PL075_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96869.1"
                     /translation="MTNNQNQDDKEKKELEEDLKDLLNQAKEINDDIKKDHEGLDEEL
                     KQIDKRSDQLVGEMNGLLEDLDKAEEKTEEELDQLILEEAEDLAVTDKEIEEILNEEE
                     "
     gene            22416..23486
                     /locus_tag="PL075_01330"
     CDS             22416..23486
                     /locus_tag="PL075_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOD96870.1"
                     /translation="MTTVLIIILIVLVIALIIIGASLISLRKRLTQQQISESSLLMLQ
                     NQINQINNTLDAKLSESSQTIREQFNESQKTIKEITQELTKVGEGQKQVFDLAKQLDQ
                     LQDILKNPKQRGVLGEYYLEVTLKNFLPVGSYQMQYELGKDENGQKLIVDAVIFIKDK
                     IIPIDSKFSLENYNRLIAEKDPIRHKELEKAVYNDLKIRINETSKYIQPKKGTMDFAF
                     MFIPSEALYYDLLINKVGSLESNTRDLIEYAVREKRVLIVSATTFAAYLQTVLQGLRA
                     FQIEESAKEIIKKVSELAKHLANYELFIQKLGNNLATTVNAYNTAQKELGKIDKDILK
                     ISGSGFIKEEAPVITPPEIEEK"
     gene            23490..24908
                     /gene="aspS"
                     /locus_tag="PL075_01335"
     CDS             23490..24908
                     /gene="aspS"
                     /locus_tag="PL075_01335"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOD96871.1"
                     /translation="MERIFINQLKNYLGQEVLLKGWVDTRRDHGKLVFIDLRDKTGVV
                     QVVFIPHSSVYEQAKELRGEWVVAVQGKVNKRPEGMENPKIETGNLEIEATDLTILNQ
                     SQTLPISIDTEGYEIGEEHRLKYRYLDLRRLRLQKNLRKRHEVILFMRNFLTNKGFVE
                     IETPILTKSTPEGARDYVVPSRLYHGKFYALPQSPQQYKQLLMVAGFERYFQIARCFR
                     DEDTRGDRQPEFTQLDLEMSFPTEEEVLNLIEDLYTKIVETLFPEKTITFKPFVRLNY
                     QEVIEKYHNDKPDLRKDKNNPNELAFAFVVNFPMFEWKETEKRWDAVHHPFTKAQNPQ
                     NLSEEEFIKYLENNPDKVLAHQYDLVCNGYELGGGSIREHNPRILQKVFEIMGNKPEE
                     IREKFGHILEAFEYGVPPHGGIATGIDRLMMILMNEPSIRETIAFPKTGDGRDLMMNA
                     PSEIDKTQLKELGIEIKNKGQN"
BASE COUNT         8692 a         3637 c         4976 g         7791 t
ORIGIN      
        1 tgtgggattt gttgttcaag ttgcggactt tatgtcagag agatttgtac tggttgcaat
       61 aaaagcaaag agggggttga gtttttgaag aaaattaatg ctaattgccc ggttttagaa
      121 tgcgctgttg aaaataaagt agatgtttgt tcgcgggatt gtgagaagtt tccttgcgat
      181 caatttcaaa actggccatt atcagaagaa tggattaaaa tgtataagga aagattaaat
      241 aactctgaat agtcattctc ttcgttattt acaacattct gacattctta agaatgtcag
      301 aatgttgtgg agaaagaatg aagaaagaat aatataaaaa tatggtaaaa ataccggtaa
      361 ttaaatcaaa aaattttatt ttaagaccat ttaataaaga ggatgaaaaa tcattgcaag
      421 gaaatattaa taataaaaaa atttatcaaa atacttcaaa tatccctcat ccttatacat
      481 taaaagatgc taaaaaatgg ataaccaaaa atttaaaaga agagaaaaag aaaaatccac
      541 aaatgattaa ttttgcaatt gaaattaatg atgaggtagt aggaagtgtg ggattgggta
      601 atataaaata cggacacaaa gcggaggtta gttattggct gggagaaaaa tattggaata
      661 ggggaataat gactgaagcg gtaaagttgg taactaaatt tggatttgaa aaattaaaat
      721 tgaaaaaaat atatggcagg gtctatattt ttaatcttgc ttccaaaaaa gtattggaaa
      781 agaatgatta taagttagaa ggtattttga aaaaagattt taagaaagat ggtaaattca
      841 ttgattctta tttgttggcc aagattaagt aataaataaa attgtttgat atgaaaatgg
      901 cgcgctttgc gcgccatttt cataccaaat tacccaatat ctttgtttat agctgcggag
      961 cttggattcg aaccagggtg tttttctgtt tatagctgcg gggcttggat tcgaaccaag
     1021 atactcagct ccaaaggctg atgtcctacc attagacgac cccgcaatga atgagggatt
     1081 ctttgaccac caaaggcggg caggccctcc tacggcgggc aggcctacca aaggcgggca
     1141 agtttgccta ccgacaggta ggcaagtgct cagaatgaca aaaacacaat cagaacgaca
     1201 gaaaaatttt cttaaaactc aataatacta aaaattaaat tttttaactt tcaatttctt
     1261 tattatattt atcctcttgt aataatcaag ttataacaaa tagattagtc gcgtcggcta
     1321 ataaagaaag tgccgtcagt gttttttatc agaacaaaat ttcggccata gtcatcttca
     1381 aagtcagctc ttcctcgacg cgaagcttca ttaaaaaata cttgaacttc gttttgatca
     1441 agacgatctc tcaaataaac gaagccatcg taagcgacct catcatcata ttggacatag
     1501 tagccataga gataatcctt tttcaacata gatttatttt taaccgccct ttaaaaaatt
     1561 ttatttattt tcactacaaa gttgaatcac aatcatttcc aaaggcaacg tttgaatagg
     1621 ggagtatttg atttgttctt tgattttcaa aagagagtca attaattttt gtaaatcatt
     1681 cattggaaaa gattgggcta attgattaac tttttctaat tgatcttcgg taaattcatc
     1741 ttgaataatt tggttaattt gagggttgat ttttataagc attatctgtc gtgctaattt
     1801 gagcacggca cggctaaaat taattaaatc aacaccattt tcataaagat tactaataaa
     1861 aataatagca tttttggtgt ctttttgcaa aagataatcc aaaaattgga ttattttaga
     1921 aaaactcact cgtcccaaaa cttcttctaa agaatccaaa tcgatttgct tataaccgag
     1981 gctaattatt tgatcgagaa aagattcggc gtcgcgcaaa ctgccttcgg ccgagacggc
     2041 aatctctttt aaggcatcag gtgtaatatt aattttttct gcttcggcca aaattttgag
     2101 acgagagatg atttcattaa gcgttagctt tttaaaatca aatcgttgag tacgagaaat
     2161 aatagttggc ggtaattttt ctggttcagt ggtagctaaa ataaaaaggg catgagcggg
     2221 cggttcttcg agcgttttga gtaaagcatt aaaagcctct ttggtaagca tgtgcacttc
     2281 gtcaatgata aaaactttat atttggattt aaccggtgca aagcgaatcc catcttttaa
     2341 ttctcgaatt tcgtcaatgc cacgattaga agcagcatca atttcaatta aatcaagcga
     2401 acggccttca tttatttcaa tgcaagatcc gcattgatta caaggttcat gttcgttttc
     2461 tttacgattt tcacaattaa cagctttggc taaaagacga gcgacagttg ttttgcccgt
     2521 accgcgttgg ccacaaaaaa gataagcatg agaaacgtgg ttagttgcta gagcattgca
     2581 caaagtttga atgattgatt tttgaccaat gacttcggca aaagtttttg gccgatagcg
     2641 gcggtataaa gctaaataac tcatatttat atttatattt taactttttc taggaaaaaa
     2701 gcaaaattaa aaataagaga aaagaattgc gatgagttgc ggcgattttt cttttagaaa
     2761 aatcgccgca acttgcagcc atcaatagat cagaaacatt ttaaatatgt taatttttgt
     2821 agctcatgaa ttaattcatg aattaattca tgagctacaa agagtaaaaa attgatttaa
     2881 aaattattta aggtaaaata gaaaagatta tgaaaagaaa aatttctacc ccgctaatga
     2941 ttacgtttgt tgtggtaata ctagtcattg tggttgtggt aatttcccaa ttttttaata
     3001 cagtccattt ttattttaat ctcgctggtc aatcacaagc gatagtcgtt gataattcac
     3061 caaaacggct agaaaatcca cccaaggata ttaaggcaat ttatttaaca gcttatactg
     3121 ccagcaatga taagcgaatg acggaaatta ttgatttagt taataaaaca gaattaaatg
     3181 cagtggtaat tgatttgaag gattacagcg gtcacattct ttttgatacc caatctgatt
     3241 ttttgaaaaa atttgggaca gaaaaagttt tgattaaaga tttagaagga ttgataaaca
     3301 aattacatca gaacggtatt tataccattg gtcggattac tgtttttcag gatccgattt
     3361 tggctaaggc acgaccagat ttggcagtaa aaaacaaaca aactaatgat ctttggaagg
     3421 ataatggagg cttggcttgg cttgatccgg ctgcacccga aatctggaat tatatttttg
     3481 ttctttctaa atcagccatg cgttatggat ttgatgaatt aaattttgac tacattcgct
     3541 ttccttcaga tggtattttg tctaatatgg cttttcctgt ttatgatggt caagttgaaa
     3601 aagcccgagt tcttaaagat ttttccgaat acttatcggc taatttgcgt ccgtggggaa
     3661 taaaaatttc agcagatgtt tttggtctga ctaccgtttt taatcacgac atgaacattg
     3721 gtcaagtttt tgagaacttc cttccctatt ttgattacat ttgtccgatg gtttatccat
     3781 cgcattatga atcaggattt ttaggttatg aaaatccagc ggcccatcct tatgaagtaa
     3841 tcaaatattc tttagataaa gctcaagaac gcatcgatca atttaaagaa aaacaaacgg
     3901 tgggagatac atctcaaaat ataaaagtag ctgttttacg gccgtggtta caaaattttg
     3961 atctgggcgc tacttataat gcctcaatga ttattaaaga aaaagaagcg gtttatgatt
     4021 cgctatgccc taattggaaa acagaaggat ttgataattg ttctaattac agcggatggt
     4081 ttttatggga tccaagaaat atttatacgc cgtcggcttt agaaaacgaa ttttaataat
     4141 agaaagatga aactttatat tatagctact cccattggta atttagaaga tatcactttg
     4201 cgcgccttaa aaattttaaa ggaggtagat tttattttat gcgaagacac aaagcattcg
     4261 ttgcgtcttt taaatcatta ccaaattaat aagccgctca tttcatatca tcagcatagc
     4321 cgattaaaca aagtgaatca aattattgat ttattgaagc aaggaaaaaa tttagcttta
     4381 atttctgatg cgggcacgcc gggtatttca gacccgggca atgaattaat taaaatgatt
     4441 ttaaaagaaa ttcatgaagt ggagattgta cctgtgccgg gcgcatcagt ggtttcggct
     4501 ctttgttcta tttccggttt taatactaat cgttttctct ttttgggctt tccaccaatg
     4561 aaaaaacagc gtcagaaatt tttaaaagaa attgctgatt ctccttatcc tgttgttttt
     4621 tacgaatcac cttatcgtat tattaaaaca ttgtcggaat tgaatcaata tgataaaggg
     4681 agtagattag aagtagtagt ggggaaagaa ttaacgaaaa tgtttgaaaa aatatatcgt
     4741 ggtaaaatta gtgaggtttt aacggcaatt caaaaagatg gagtgaaggg ggagtattgt
     4801 gttattgttt cttgcgagga tagtaaatca aatttgacaa agaataaaaa ataaagaaaa
     4861 taattgaagc gaattttaaa aatttagttt gaaaggagaa aagatgtttt ttatttgtgc
     4921 gattgaacgt cgaaaaaaag agaagacaaa ggattttttg gtcagagtgt ttaaattcaa
     4981 aaaaatggta gaaaaagaat tgcgatgcaa attaaagatt gttgtgtatg ttgatgatca
     5041 acaaatttcc tgggcaaaaa ttcttaatac gagacaaaaa catccaaacc caaatttatt
     5101 aaaacaaaaa taaagggctc ctaagccctt tttctttttt taaaaatagt tataataaaa
     5161 aagtcgctga aaaaagaaaa ttatgtcttt tgaacaacct aataatttta ataaaattga
     5221 atatgatcca gaaatggaaa aacaggaacg ttggagaggg gaaatagaag cagaagtcgg
     5281 aaaagaaatt tcagaaggac agtatgaaaa agtagaggtg caaatgctta aggaagaggt
     5341 taaacatcaa agtaaggata tgtcagacga agaggcttac agaattgctt tagaaatttg
     5401 gaaacaaata gaagttgata aaagatttaa tcaaaaagtg caagaagaaa aagaacgcga
     5461 ggaaaaaatc atcgataaat aaaatccgtc ttagaggcgg atttttcata atcgcatctg
     5521 agcatttttg tctgttattc tgagtgtttt tattttttgt cattcagagt gtttttgttt
     5581 tctgtcattc tgagagcttg ccaaaggtgg gcaggtacaa cccccgccta accgcctgcc
     5641 tgccggttaa gcggggcggg cagggaagaa tcccatccac agcaaaaacc ccacggtcat
     5701 tctgagcgtc agcgaagaat cccacccaca gcagtaatag ttgcacaatg ctaaaagcca
     5761 cgagatcctt cactaacgtt caggatgacg acgaaaaaag gatgacgata aaaaaatagg
     5821 atgacaatgg aaaacagggc gacaggaggg ttgaagacga ggatcttttt attgacaagc
     5881 atctataaat ttgataatct aaaaaaagaa aagaagcgtt cttttaaaat tataaaatta
     5941 attaaaatta aaaggaggta aaaatggaag aatctttcgt ttatgctggt gggggcattt
     6001 cggggttaac ctatgaggaa tgcgttggtt ggcgtaagta tgtggcgagt aaaatgccat
     6061 cttacatcca cattctttcg ccgatgcgcg ggaaagaata tttaaacaat ggaaaaatta
     6121 tcagtggctc gtatgaggag catccattat gttctcaaaa aggaattctg tgtcgagatc
     6181 attatgatgt tcggcgtgct aatttagtgc tgatgaattt tttaggtctc caagtggttt
     6241 cgattggttc aattgtagaa atcggttgga cgtatgccta tcggcatccc ctgattactt
     6301 taatggaaaa aaacaatgtt catgatcatc cgatgatcag ggaaatatca aatttcattc
     6361 ttactgatct tgacgaagct attgaactga tcattacaat tttgactcca taaaaaaata
     6421 ataacagacc aagttttcct tggtctgttt tgttttgtgg accgtagcgg attcgaaccg
     6481 ctgacctcct cattgcaaat gaggcgctct agccaactga gctaacggcc cctcactttt
     6541 ttaatcttaa tcaatttggt tccatttttc aaccttttag aaaaagaaat tctgagttat
     6601 aataataaaa tatgagcgat caaaaagaca aattgcaaaa atttattaag aaatactatc
     6661 agaaagaaga aaaaaacata gagcaaatta agcaacgaat tgaaaaagaa aaagaaggga
     6721 gacagtttat tgatcgagcg atatattatt tatttcaaat tcaggaggaa gtaagagaaa
     6781 ttattgataa aaattatagt gggattgttt ttgatgaaaa agacgtaata ttagaagaac
     6841 cgccgctttt tatcaagagc gatttgtgtt ttagttgctt ttcaatggca aagaagttaa
     6901 aaaaatcgcc cgtgcttcta agcgaagaaa ttgttaattt aattaatgct tccactgaaa
     6961 aagaatttat tgctgaggct acgagcgaag gcccttatat taatcttaat ttaaaatcag
     7021 aaatttatag caaagttcta gcccaaatta ttaatttagg tgaacagtat ggagaaagca
     7081 atttaaattc tggagaaatc attcttattg attattcctc gcccaatatt gcgaagccaa
     7141 taggattaaa tcatctaccc tcaactatta ttggccatgt tttaggaaat ctttattatg
     7201 caacaggtgc cagtgttatt cgacataatt atttaggcga ttgggggaca aattttggcg
     7261 ctttattatg ggcttatttt cattggggtg atgataaaaa aattcaagaa aatccagtta
     7321 tagaattaaa aaatttatac gtgcgatttg ttgaagaggc gagtcaaaat gaaacaatga
     7381 ttaatgaagc gcgcgaactt tttaatcaat tagaaaacgg cgacgaaaaa cttttaagaa
     7441 tttggaaaaa atttcgcgat ctgagcatca aagattttaa aaaagtctac cgacgtttgg
     7501 gtataacttt tgattgttat ttaggtgaaa gctatttttt ggatcaaaca gataaagtaa
     7561 ttaatgaggc tattgaaaaa ggggttgcaa aagagaatag cgataataaa ttaatagtgg
     7621 tagattcatt tgaggggctg ccgtcatttt tattaagaaa agaagacggc tcaactcttt
     7681 atcttagccg tgatctcgcc gctttgaaag tgcgttttga actttttaat ttagattggc
     7741 ttttgtatgt tgtggggagc gaacaatctc ttcattttca gcaattattt gctttagcag
     7801 aagcagtggg ttatttaaat gaaaagaaaa aagccaagca tattccattt ggcctaattc
     7861 taattgaagg aaaaaagatg gcgacaagaa aaggaagcct tattgaaatg gacgaattga
     7921 ttaacgaggc tattaaacga gcagaagatt taattacaaa gaaaaattcc gagatgagcg
     7981 aatcggaaag taaaaaaatt gccgagcagg ttggattagg tgcggtgatt tataatagtt
     8041 taaaacaatc acgattgcag aatattaatt ttgattggga aaaggtttta aattttgaat
     8101 ccgcgagcgc cccttatctt caatatagtt gtgtacgcat taaatcaatt ttgagaagaa
     8161 tagatttgaa gggagatata aaaatcagcg gcgatcaatt gacaaaagct gtttttgaaa
     8221 atccaacaga atttgaaatt ttaaaaaaat taatgctatt tccaatggtc attattaaag
     8281 cccaaaaaga agacgctccg catctgcttt gcacttattt agaagaatta gcgcaattat
     8341 ttaataaatt ttatgataat gtttctgtta tcaaaacaac agatgaaagt ctcaaaaatt
     8401 ctcgagtgtt tttgattaaa gccacgttgc aaattttaga aaaaggattg gcattattaa
     8461 atattagagt gcctaataag atgtaagaaa agattaaaga aattgaaaag cggccagctt
     8521 tgctggccgc ttttcaaaca cttgtttatt ccttctacaa tgtaggagta ggggtgattg
     8581 tgggagtggc ggttggtgtt agtgtgggag ttggagttgg agttgttgtt tcggttggcg
     8641 ttggagtaag agtagggctg gcagttggag ttggtgatgg tgatgcttca ggcttgcgag
     8701 agaagaggcg aacaatttta ccccaaagag aggagtattg ttcttcgtta gtgagataat
     8761 cgtttaaaac aatttgttgt tgagtaagcg cgttaattaa ttcctgcacc atctgttgct
     8821 ctcccgcatt ggttaattga ttttttaact gttctaattg ctgaatgcgc aattgaattt
     8881 ggtccagatg tttttttact tgattaatgc ttgttaaatc aggaccaaaa atcgatttga
     8941 gccatttagg gcgagaatca attttttcta ttcgttcttg aatttgctgt tgaatctctt
     9001 tttggtttct agcgatttct cgtacttgtt gtccaatacc agtaatttca gttgttgtag
     9061 cggttaataa ttcttcaact ttttgggcaa caatactcat atgttcacga gcggtttcac
     9121 ttcttggatt aagtgtttta ttagggcgag gaacaatcgt tttgggcgct ggcgttgttg
     9181 ttgtgacttg attttgctct tctgtttgtt gttgaatatt cgtttctcct ccctggttct
     9241 gagtttggat ttgatttttt tcttgaatat ttttttgtaa acccggtgca ttaatgggca
     9301 aaaccggttt ttctgttatt tgaggagata gagcaggggc agcagaggta gattctggtt
     9361 tgttgccggt ttgattggcc gattgagaat ttgttgtcgc ggccatcaaa ggaactgtaa
     9421 taaacagcga aagactaata atcaaggcaa tttttttatt catatatttt attatttgac
     9481 ttttatttat ccgaccattt tatttatccg acctttattt tatgtcgggc tgccgggaat
     9541 tgaacccggg tcgcacccac cccaagggtg tatactacca ttgtactaca gcccgcaaaa
     9601 aatacaagta ttaaagatag aggcctttat ttaaaatgga taaaaaacag gaatagttat
     9661 tgaaatagca gtatttaact aacatcttta ctgcttgatt aataaatatt gacttgatta
     9721 aaaggaatcg gcttttattt tattgcttcc ttttttaagt ttttaatgca acgagcgcag
     9781 actttaactc ttttgcctga tggcaaacga aaccattgta agttggcctt tgtttttttc
     9841 cgattaaccg gatcatactt acttctaatt ttttttcgtg ctatactgta agtataagat
     9901 tttccacaaa tagcgcattg acgcataata gcatttcttt catccgacta tttttttatt
     9961 ttaacctatt ttaaaatttt ttcaaattat tatgagtgga tctattttaa tttttcaatt
    10021 aattatttta cttttttcag ttattttgca cgaagtgagt catggatata cggcttatat
    10081 cttgggtgat gatacggcaa aagttaatgg ccggttaacg ttgaaccctt taccacattt
    10141 agatctctgg ggttcttttt tatttccgat gatttcttat ttaattagcg gtggcacatt
    10201 tatttttggt tgggctaagc cggttccttt taaccctcta aaattaaaaa aacctaaaag
    10261 agatattggg ttggttggtt tagccgggcc tttggctaat ttatttgtgg ctctttgttt
    10321 ttctattatt attcgattgg ctcatatttt taattgggcg tttttaacgc ctctgtttac
    10381 ctttttttct attattattt atctcaacat tcttttggca attttcaatt tagtaccaat
    10441 tccgccgctc gatggttcca gggtaattta ttctttattg ccaaggaagt gggaaaaatt
    10501 ttattggagt ttagaacgat tcggttttat tttagtcatg atttttgttt tattcggatt
    10561 tcaattaatt attccaatca ttgattcgct ttttaacctc tttgttggtt aaaagagcaa
    10621 cagttttaaa aattttgtaa agaaaaaagt tatccacaat ttctattatt gaagggaaaa
    10681 tcaacgcagt ctaaaattta gataaagatt ttaaaaggtc aaaatttttt cgaaaaattt
    10741 tttcaaaaat gaaaaataaa attctaaaag tgcaaaaacg tgatgggtcg attattgatt
    10801 ttgatgaaac acgaatccac gatgctattt acaaggccat tacagctact gatcaaggcg
    10861 atggcaaaag gactaaaaaa ttaactgaaa aagtcattga tttattaaat cgaagattta
    10921 aagccgatga aattcctcat gttgagcaaa ttcaagatat tgttgaagaa gttttaattt
    10981 tggaaggact gaccgaaacg gctaaagcct acattcttta tcgtgaacaa agaagaagaa
    11041 ttcgtgagtc tgttgctttg gttgatgaat cgattgagat ggttgataaa tatattagcg
    11101 aaattgattg gcaagtgcat gaaaatgcca atatgaccta ctcgcttcaa ggtcttaatc
    11161 aatatattat tgctcaagta gaaaaaaagt attggttaaa taaaatttat cctaaagaaa
    11221 ttagagaggc tgcgcagaat gaagattttc atattcataa tttagaaaca ttggcacctt
    11281 attgtatggg atgggatctg atggatttac ttctcaaagg ttttggtggt gtgccgggaa
    11341 aagtagaatc tcttccgccc aaacattttc gaaccgccct tgggcaattg gtgaattttc
    11401 tttttacact ccaaggagaa accgccggtg ctaacgcagt ttctaattta gatactctgc
    11461 tggcgccatt tattcgttat gatcatttat ctcagcgaga agttaaacaa gctcttcaag
    11521 aatttctttt taattgtatg gtgccgacac gagttggttt tcaaacgcct tttctgaatg
    11581 tcagtttaga tattaaaccg ccacaatttt tagcaagtca atctgttatt attggtggcc
    11641 agcctcaaaa agaaacatat ggtgattttc aagaagaaat ggatttattt aataaaactt
    11701 tttatgaagt aatgctcgaa ggagatgcta agggccggcc atttactttt ccgattccca
    11761 cggtttctat tactaaagat ttcgattggg acaatccagc attaattcca atgtgggaag
    11821 ctacagctaa atatggaatt aattattttt ccaattttgt aaattctgac atgaaaccag
    11881 aagattttcg ttcaatgtgt tgtcgattgc gtttagacaa tagagaactg tataaaagag
    11941 gaggaggatt atttggcgca gctcctctaa cgggctcaat tggcgtttgt acaatcaata
    12001 tgccacgaat cggctatctt tctaaaacaa aaaaagagta ttttgaacgt ttggcgcatt
    12061 taatggattt agccaaagaa tcattagaaa tcaaaaggaa aactctagaa aattttattg
    12121 aaaaaggatt atatccttac actaaatttt atctttcttc agtcaaaaac attcgtggtc
    12181 aatattttgg taatcatttt tcaacgattg gtttaatggg catgaacgaa tcacttctta
    12241 attttttggg cgaagatatt tctaccaaac gcggcatcaa atttacttta gaagtattag
    12301 attttatgcg agatcgtctg gttgattatc aaaaagaaac aggcaattta tataatcttg
    12361 aagctacgcc gggtgagggc acgagttatc gtcaagctaa agtggataaa gaaaaatacc
    12421 cggatattat caccagtggc accaaagaag ttccttatta tactaattca acaatgttgc
    12481 cagttaattt tactgatgat atttttgaat ctttgaaatt gcaaaatgat attcaaacaa
    12541 aatatacagg cggaattgta actcatatct ttttgggcga acgcctttca gatattacaa
    12601 ctacaaaaaa tttgattaga acggtttgtt ctaaatttaa cattccttat attactttga
    12661 cgccgacgtt ttctatttgt ccgacgcacg gttatataaa tggtgaacac ttcttttgtc
    12721 ccaagtgtgc ggttaagcaa ccatgcgaag tctattcgcg aattgttggt tatttgaggc
    12781 cggtgagcca atggaatttt ggtaagcagc aagaattccg tgatcgtaaa gaatttaatg
    12841 taaagaaaat taaattgcca gtttccaaaa aataactaac aataatggaa ataggaggtc
    12901 ttcaaaaaac aacacttatt gattatccgg gtaagattgc ctgcacgatt ttcttaatag
    12961 gttgtaattt tcgttgccca ttttgttatt cggctgagct tgttttgcca gaaaaaatta
    13021 aaaatcagcc acgcattgat aaaaaagagt tgtttcgttt tttaaaagaa agacagaaat
    13081 atttggaagg cgtggttatt tgtggtggag agccaaccat tcattctgat ctgtctgatt
    13141 ttttgttgca aattaaaaaa atgggttatt tgattaaatt agataccaac ggcactcaac
    13201 cagatgtaat aaaaaaatta attgataaaa aacttgttga ttatattgca atggacatta
    13261 aagcgccact tgaagaagag aaatatgctg cagcgagtgg tgtcaaggtt gatttagaaa
    13321 aaattagaca aagtatagcg ctaattaaaa attcgggtat tgattatgaa tttcggacaa
    13381 ctgttgttcc aactattcat caattagaag atattctcaa tattgctcaa gcgattgcac
    13441 ctgcccaaaa attttatttg caaaattttc gacccgagaa aaccttaaat gaaaatcttt
    13501 tgcaagttaa acccttttcg gaaacagatt tagaaatgat actagaaaaa attaaaaatt
    13561 tatttgaaga gtgcaagata agataaatat attcagtagg gtggcggcga actgttggcg
    13621 gcgaaatcta aaagatttcg ccgccaacat gccgattggt tgccgttaat tattaataga
    13681 cgattattat agttattaac aattagcact cttgacataa gagtgctaat tgaatataat
    13741 tgattataaa aatatgctga atgaacgaca gaaaaaaata atagatttga ttattaaaga
    13801 atacagtaaa aaccagaaac cagttggttc aaggtatttt tgccgaaaat attttcctga
    13861 tttgagtcca gcaacgatcc gacgagaatt ccatattttg actgatcaga aatatttgtt
    13921 ccaaccgcat tgttccgctg gccgattgcc taccgataag gcttatgatt ggtttgtgca
    13981 agaaaattta ataaaccgtc cgagtcaaaa agaagaacta gaccattggc aaaaaaagct
    14041 cgttgattta aaaaatagtt ctttttccga aacagcaaaa agaattgccg ctctgtgcga
    14101 tagtttaaca attggttact tgccttggga gcaattaatt tataaatacg gctttaaaaa
    14161 tctttttgcg cgtcttgtgg aaataaattt gaatgaatgg aaaacgattt gggaagacat
    14221 agatcaattg gatgatcgct tagaaaaaat tgccaatcat ttaatggaag aagaattaca
    14281 gattttttta ggagaagata gtccattgac gcgtgataga aacctcagcg ttatttcgtc
    14341 ggttatacct tataatcgga gaaaaacgct tcttatttta attggtccaa agaatatgat
    14401 ctgcgaaaaa aatttaaata ttttagaagc agctcgcaat attttaaatc agattaataa
    14461 tcaacaatat ggcagaacaa aataaaaaca atcaaatgga tgctgaggaa aaagaaatca
    14521 aagaaaaact gcaagattta acgcagaaat tagaggaatg cgaaaaatta aaagaagaat
    14581 atttgaatgg ttggcaaaga gaacgggcgg catttttaaa ttatcaaaaa gatacgagca
    14641 aaagaataga agaaataatt aaaatggcca atgaaggatt aatcagagaa ttattgagcg
    14701 tgttggacag cttcgatatc tcagtgaact ctttaaatgt agaaggctta acgcctactg
    14761 aaaaaaatat tgttcgcggc atagagctta ttagaggaca attattagat gtgctgaaac
    14821 aacaaggatt aaaggaaatt caagcgatcg gaagtcaatt tgatcctcat ttgcatgaag
    14881 ctattgatat tgtaaaagga gggaaggatg gtgaagttgc agaagaacta atcaagggtt
    14941 atacgctgaa cgataaagta attcgtccag caaaagtaaa ggtctttaaa gaagataaaa
    15001 agtagcaatt tttataataa aaggtcacga atttattaaa ttattaaaca ataaaatata
    15061 tgagcaaaat attgggtatt gatttaggaa caacaaattc ggctatggct attatagaag
    15121 gtggtcagcc gaaaattatt gaaaacaaag aaggaatgcg taccactcct tcggttgtgg
    15181 cgattgccaa aaacggcgag cgcctcgttg gtgttttagc aaaaagacaa tcagttacca
    15241 atgcccaaaa caccattttt tctattaaaa gattaattgg tcggaaatat accgattcgg
    15301 aagtgcaacg cgataaaaag tggttgcctt atgaaattag agaatctgca agtggtggcg
    15361 ttgaggtaaa aatgggtgat caatggtatc gaccagaaga aatttcagca atgattttac
    15421 aaaaattaaa gacagatgca gaagaaaagt taggtgaaaa aattgaggaa gctattatta
    15481 ctgtgccggc ttattttgat gatagtcaaa gacaggctac aaaaaacgca ggagaaatag
    15541 ctggttttaa ggttcgaaga attttaaatg agccaacagc tgcggctttg gcttatggat
    15601 taaataaaaa gacaaatgaa agaattgtgg tttatgattt tggtggtggt acttttgata
    15661 tttccatttt gcaagtttct gaagatacgg ttgaggtaat ttcaacaggt ggagatactc
    15721 atttgggagg cgatgatttt gatcaacgca ttattaatta ctttgtagaa gagtttaaga
    15781 agcgagaagg aattgatctt tcaaaagatc ttttagctgt gcagcgctta aaggaagcag
    15841 cagagcgcgc caagcatgaa ttatcaaccg ttcttgaaac agacgtcaat ttaccattta
    15901 ttacctccga cgcttctggc cctaaacatt tcgaaatgaa attaacacgt gcaaaattag
    15961 aagaattggt aaaggattat attgatcgct ctgttgaatt agtcaagcaa actctggcag
    16021 aaaaaaatct gaaaacttca gatattgatg aagttatttt agtcggtggt caaacacgaa
    16081 tgcctgctat acaagaagca gtgaaaaaat tttttggcaa agaacctcac aaaggtatta
    16141 atccagacga agtagtagca atgggagcag ctgtcgaagg tggtatttta cagggcgaag
    16201 tcagagatgt attgcttttg gacgttactc cgctttcttt gggcattgaa acgcttggcg
    16261 gcgtgtttac aaaaatcatt gaaaagaaca caaccattcc tgtttcaaag tctcaaattt
    16321 tttcaactgc cgctgataat cagacgtcgg ttgaaattca tgtgcttcaa ggcgaaagag
    16381 aaatggctgc tgataataaa acactggctc gttttattct ggacggcatt ccgcctgctc
    16441 cacgaggcgt gcctcaaatt gaagtaactt ttgatattga tgcgaatggc attttgaaag
    16501 taactgctaa agacaaggca actggcaagg aacaatctgt tcgtattgaa gcatcaaccg
    16561 gtttgactaa agaagaaatt gaaaagatga aaagagaagc tgaacagcat ttaagtgaag
    16621 ataaaagaaa gagagagttg gttgaaacta aaaatcaagc agaaaatacc atttatcttg
    16681 ctgaaaaaac tttacgcgaa gcaggagaaa agattactcc tgaagatcgc aaagaagtgg
    16741 aagataaaat taatgattta aaacaagtgc taggagggga cgatctcgaa aatatcaaaa
    16801 agcgattgga agatttatct ttggcaattt caaaaattgg tgctaaaatg tatcaaggtg
    16861 gtcaacaaac taataatccc aataatcccg acaattcttc atccgatgat aacaaaccag
    16921 aagatgcaga atttaaggat taatagggaa tcctatggct atcttggaaa ttttttctag
    16981 taccaatcat tctacagaaa aatctaacta ttggtggaaa attcttattt ttaccgtttt
    17041 ggccttaatt acagcttcat tggctggagc gagtttgggc tatctttttt tggggaaatc
    17101 atttttaggg ttttcctcat tttggggatt cttgattttt ctttgtctgt ttggcctttt
    17161 ctttattatg gagagtttgt ttgctcaagg agcgcgtttt ggagcggttt tgctggaaag
    17221 cttcattttc gcactgagtt tttatgcgat taggattgga ttttcaaaat ttaatgggtt
    17281 tatgttgatg tggagcttgg gcgtatggtt gttgtttctg ctatgtttgt ggggtgggcg
    17341 atttaagatg cgtcagcgaa aatccgattt gctacgatta aaatggaatg aaatggtcaa
    17401 aggcggcttg gcattggtat tgataggcgt taatttattt atctgtttgc aatggatggg
    17461 caatgttctt attcagccag atcgattatt tactcaaaaa accgttgata tgattttaaa
    17521 accagctact ccgctattgc aagtctattt cactgatttt gatccttata tgactgtaga
    17581 cgcctttaca aaaagcgtta tttcggaaca ggtagaatca atgtttgttc aatccgaaga
    17641 tagtaagaat tacattccta aaaatataat agaacagcaa aaaaaggatt tgattgaaca
    17701 gaacagtttt gcctttaaaa gtcagttagg tgaattactg ggatttcccc tgagtggtac
    17761 tgaacaaatt aacacagtag ttttccaatt tttatggatg aaattccaat ctttaagcat
    17821 tgcaacgaag cagtatatta ttttaggaat ttcattttta atgtttcttg tttttcgcat
    17881 tctagctatt tttataggct gggtaattca aatgatcggg tggtttatct atgagttatt
    17941 attggcagga ggttttggag cagtaaaaat tgaaatgaaa aataaagaag atcttgtttt
    18001 attttagaat tttttaattt atggagaaaa aagattatta taaaatatta ggtgttaatc
    18061 gtaacgcttc tcaagaggaa atcaaaaaag ctttttacaa attggcccat caatatcatc
    18121 cggataaggc cggtggtaac gaagaaaaat ttaaagagat taatgaagcc taccaagttt
    18181 tatcagatga gaataaaaga cggcagtatg atcaattcgg tcaaactttt gaaggagcag
    18241 gcgcgagtcc aaattggggt tttgactttt ctaattttgg tcagggcggt tttaacaata
    18301 gtttcggatc aaaggtagat tttgattttt ctgatttagg ccttgatgaa gatttgggtg
    18361 atatttttaa taatattttt ggtggattta ataccggtca gcgaaagaga aaaaaagttc
    18421 agtacggatc agacgttcgg ttgatgatcg aaatttcttt ggaagaggta gcaaccggcg
    18481 tggtcaaaga aatcgaatac aatacatttg taaaatgtga tcattgtcaa ggtcgtggtt
    18541 acgaagaagg atcaggattt aaaacttgtc ccacttgtaa tggtcgcggt gaaattaaag
    18601 aaatacggca gagttttttt gggtctttga gtcaggtgaa aatttgtccc gaatgtggtg
    18661 gtgaaggtaa aattcctgaa aaaatttgtt ctttttgtaa aggccatgga cgtgtaaagg
    18721 gtaaaaaaag aatttcattg aaaattgagc cggggattaa tgatggtcaa attattcgcg
    18781 ttgctggtgg tggcgaggat ggagaaaaaa atggaccatc cggtgatctt tatgttgaag
    18841 tacgcgtgaa acctcactct cttttcaagc gcgagggggc agatcttatt tatcaaactt
    18901 ccattcctgt gcttgaagca attctcggga cagaaaaaga aattcccacc atttatggca
    18961 agccgattat tcttaaaatt ccggccggta ttgactcggg aaaaatttta cgtgttcgca
    19021 ataaaggatt gccttatttt ggtcggcgtg gttatggcga tctgctagtt cacattaaag
    19081 taaaaattcc caaaaaagtt tcttcaaaag ccaaaaaaat tttagaagat ctcagaggtg
    19141 aattaaatga ttaaaaatat gaacattgct ctcacttata atttacgtcg cgtgaaaaaa
    19201 ctatccaaaa ataaaaaagt aattcaaaat attgattttg acgatccaaa aaccatccaa
    19261 gggattaaaa aatctcttga atgtttaggt cacaaggttt ttcttgttga ggccgatgaa
    19321 aacgcctact tcaaatttaa aaggttacaa aagcgtattg atttagtttt taattatagc
    19381 gaaggattgc acggtaaaga tcgtgaagct caaattccag ccatgcttga aatgctccga
    19441 attccttata cgggcggatc acctttggcg tatgccattg gtttgaataa aaccaaaacc
    19501 aaagaaattt taagttatca tcgtattgct acgccaaaat ggcaagtttt taaaaccggt
    19561 cgggaaaaat tagatcgtca tctaaaattt cctttaatcg taaagcctca atccgaaggt
    19621 tccagcaaag gaatttttat aaaaagctta gtcaaaaacg aaaaagaatt aagaaaaatt
    19681 gttaaagagg aaattaatga ttaccatcaa ccgataatag cagaagaatt tttgtcaggt
    19741 cgcgaattta ctgttggcgt gttgggttgt ccgccaaaag ttttgcctat tattgaagta
    19801 cgttttgatg aattgccaaa aggtattcct aaatttgatc attttgaggc taaatggctt
    19861 tatgataatc cgaaatttaa agctgatcct ttactatgtc cagcgcccat tacagcaaaa
    19921 ttaaaaaaag aaattgaaga tatggtttta aaagcctttg aagttttaga aatggccgat
    19981 tgggctagat ttgatttgcg tttagatcaa caagggcggc caaatatttt agaagtaaat
    20041 tgtccagctg gtttaaatcc agatccaaaa gaaaattcgc gttttccgcg cgctgctcgc
    20101 gtggctggtt taagctttga agaattactg caggagatca tcaattctgc tgttaaaagg
    20161 caggagataa aataaaagtt ttatacttaa agcaaaaaga ttgttacatc gatcgtatct
    20221 aaaacgaatt gatgttagtt ttacatcaat tattcctatt gtgaaattga taaaaagatt
    20281 tttaaaaccg actggcgaag cggtcatact aattaaacct cagtttgaag taggaagagg
    20341 taatttaaat aaaagaggcg tagtaaaaga tctttcaaga tctcagcagg cagtaaaaga
    20401 aattctcaga attgtacaga gggaaggcca tagaattaaa ggcccgatag cctccagtct
    20461 taaaggaagg gcaggaaatc aggaattcat tctttattta aaaatgtgat aaaataaggt
    20521 aaaaagaggc tttttcaagc agatttttga tttgactttt atctttttat gtgctataat
    20581 aaaatatagg ggattaaaaa ggaatagact agattttgct taattttaaa ttcggaatta
    20641 taatataaat aagtcgataa aaaataaaaa catgagtacg ccagaagtta aaaaaacact
    20701 ctcagtcgaa agcaaagaag cgagtgcttc atcatccaaa acaggaacga cacctgtgtt
    20761 tcgcattgat gaaattctaa cgaaggccgg tcgcagttta tttgataaaa tttctgatag
    20821 tggcaaagct gttgttcatg gtctttacga gggagcttat aaatcaccgg gcatcaatcg
    20881 attggttgga aaaatggaaa ttgcttataa tcaatttcgc ggtgataaat atgaaaagaa
    20941 agcggctaaa tttaaaaata aattagatgg agttgatatt aaaataaatg cttttcaaca
    21001 agccaaagag aaccttgccg gtgtgataaa agatttaaag cagcaaaata ttccagctgc
    21061 cacggcatta gaattaaaaa tcaaagatat tgaaaagcaa attaataaat tagctgataa
    21121 gcgcgacaga attcaaacta aatttgaaga gaaagaaagc agaagaaatt tacgaattaa
    21181 taagagagat caaatcgcaa atagattaat agaatattat gaaaacaaac tcgaaccgtt
    21241 tgagaaaaaa ttagaggaat taagaaaaga gcaagaccat attgatttat tgatgatggc
    21301 gactgaggtg aagcacaaga atcgtgaagt tgcactggac aaacttcaaa agaagcttga
    21361 ttttactatt gaaaattata agcgacttgg gatgtcagaa agaaagattc gtaaggatga
    21421 agcggtaaaa gcttttaatg aacaaattgc taatgcacgc aaagaaatgc aattagaaaa
    21481 taaaaagcta caattagcca aggataaatt ggatactaaa attgctaagg ttgatgctaa
    21541 agccaatcct tatcgcgata gacgagaaga atttgtgaga gtcacaaagg ttcgtccaat
    21601 agaatttaac gttccaggac gagaaaaagg gcaaccacat gaggggaagg aagaaattaa
    21661 aactcatgag cgtaaagaat cagaggtaaa aaagacagag tccgcgtcta aagtcgaagg
    21721 aaaaattgag acagcatttg aagaggaaga agaaaaagaa aaatttccag tagcagcgta
    21781 tcttgccacc ctcaatgatt atttactaaa aaattatgac aaaacgtacg ttattaataa
    21841 aaaagattat tatgcccaaa cgcaattatc acgatacgat aagatggaag tagctgattt
    21901 tagaagaatt ttattgcaat atgctaaatt taaaagaatg ccaacccaaa aagtagacat
    21961 ttacgaaaaa gccataaata atcttttcga aaaaaataaa tagtcggtgg taattaaata
    22021 aaaaaatatg accaataatc aaaatcaaga cgataaagaa aaaaaagaat tagaggagga
    22081 cttaaaagat cttttaaatc aagccaaaga aattaacgat gatattaaaa aagatcatga
    22141 aggtttagat gaagaattaa agcaaattga taagcggagt gatcagttgg ttggggaaat
    22201 gaatggtttg cttgaggatt tagataaagc cgaagaaaaa accgaagaag aattagacca
    22261 gcttatttta gaagaagctg aagatttagc tgtaacagac aaagagatag aggaaatttt
    22321 aaatgaggaa gagtagcaac taattaggag gagctccgcc ggttctggcg gggctcctct
    22381 tttgtttttc taattttcta tttataatag gaataatgac aactgtatta attattattc
    22441 ttattgtttt agttattgcg ctaataatca tcggtgcttc tttaatttca ctacgaaagc
    22501 gtttaactca acaacagatt agcgaatctt ctcttttaat gcttcaaaat caaatcaatc
    22561 aaataaataa tacgttagat gctaaattgt cagaatcaag tcaaaccatt agagaacaat
    22621 ttaatgaaag tcagaaaact attaaagaaa tcacccaaga attaacaaaa gtgggcgaag
    22681 ggcaaaaaca agtttttgat ttagccaaac aattggatca acttcaagat attttaaaaa
    22741 atcctaaaca gcgtggtgtt ttgggagaat attatttgga agtaacgcta aaaaattttt
    22801 taccagtcgg ctcctatcaa atgcagtatg aattgggaaa agatgaaaat ggtcagaaat
    22861 taattgttga tgcagtgatt tttattaagg acaaaattat tcctattgac tccaaatttt
    22921 ctttggaaaa ttataatcgt ctaattgccg aaaaagaccc cattcgtcat aaagaattag
    22981 aaaaggctgt ttataatgat cttaaaattc gcattaacga aacatccaaa tacattcaac
    23041 caaaaaaagg aacaatggat tttgctttta tgtttattcc atccgaagct ttatattacg
    23101 atttattaat taataaggta ggtagtttag aatctaatac ccgtgatctg attgaatacg
    23161 ccgttagaga aaaaagagtt ctcattgttt cggccacaac atttgcagct tatcttcaaa
    23221 ccgtgcttca aggattgagg gcatttcaaa ttgaggaatc agccaaagaa attattaaaa
    23281 aagtttctga attagctaaa catttagcca attatgaatt gtttattcag aaactaggta
    23341 ataatttagc aacaacagtg aatgcttata atactgctca aaaggaatta ggaaaaatcg
    23401 ataaagacat tttaaagatt tctggcagtg gtttcattaa agaggaagcg ccagtcatta
    23461 ctccgccaga aatcgaagaa aaataaatta tggaaagaat ttttattaat cagttaaaaa
    23521 attatctcgg gcaagaagtt ttgcttaaag ggtgggtgga tactcgccgc gaccacggca
    23581 agttagtttt tattgatttg cgagataaaa caggagtagt tcaagtagtg tttattcctc
    23641 attcttctgt ttatgagcag gcaaaggaat taagaggtga atgggttgtt gctgtgcaag
    23701 gtaaggtaaa taaaaggcca gagggtatgg agaatcctaa aatagagacc ggtaatttag
    23761 aaattgaagc cacagattta actattttga atcaaagcca aacattgccc atcagtattg
    23821 acaccgaagg ctatgaaatt ggcgaagaac atcggctaaa atatcgttat ttggatttac
    23881 ggcgtctacg tttgcaaaaa aatctgcgta agcgccacga agtaatttta tttatgcgta
    23941 attttttaac caacaaagga ttcgttgaaa ttgaaacacc tatcttaacc aaatcaacgc
    24001 cggagggagc acgcgattat gtagtgccat cgcgtttgta tcatggtaaa ttttatgcct
    24061 taccccaatc gccacagcaa tataaacaac ttttaatggt ggcaggcttt gagcgatatt
    24121 ttcaaattgc gcgttgtttt cgtgatgaag atacgcgggg tgatcgtcag ccagaattta
    24181 ctcaactgga tcttgaaatg agttttccaa ccgaagaaga agttttgaat ttaatagaag
    24241 atctttacac taaaattgtt gaaacattat ttccagaaaa aactattact tttaagccat
    24301 ttgtgcgttt aaactatcaa gaagtaatag aaaaatatca taatgataag ccggatttaa
    24361 gaaaagataa aaataatcca aacgaactag catttgcttt tgtagtaaat tttccaatgt
    24421 ttgaatggaa agaaacggaa aagagatggg atgcggttca tcatcctttt actaaagcgc
    24481 aaaatcctca aaatttgtct gaggaagaat ttataaaata tttagaaaat aacccggaca
    24541 aagttttggc tcatcaatac gatttagtat gcaatggtta tgaattaggc ggtgggtcaa
    24601 ttcgagagca caacccgcgc attctccaaa aagtttttga aattatggga aataaacctg
    24661 aggaaataag ggaaaaattt ggtcatatcc tagaagcatt tgaatatggc gtgccacctc
    24721 atggaggcat tgctacagga attgatcgtt taatgatgat cttaatgaat gagccaagca
    24781 ttcgtgaaac aattgctttt ccgaaaacag gtgatggacg tgatttaatg atgaatgcac
    24841 catcagaaat tgataaaact caattaaaag agttggggat agagataaaa aataaagggc
    24901 aaaattagta aaaaagaaga tgaaatggac ggcggaaaat ttcaaagaaa ttttccgccg
    24961 tcttgttatt ttagctcatg aattaattca tgagctaatt catgagctaa taaatagtct
    25021 ggcggtggaa aattctaaaa aaaatttttc cgccgccccc tcactttttt cgtatgcatt
    25081 tttactgtca ttctga
//
LOCUS       DAOSMC010000007        24777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_2551_length_24777_cov_21.962867, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000007 DAOSMC010000000
VERSION     DAOSMC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 24777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24777
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2551_length_24777_cov_21.962867"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PL075_01340"
     CDS             <1..615
                     /locus_tag="PL075_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96872.1"
                     /translation="MEQLVRSGSIDIVVVDSVAALTPRAEIEGEMGAQHIGLQARLMS
                     QALRKITAIAAKTKTVIIFINQIRMQVGIMFGNPETTPGGRALKFYSSVRIDIRRTAQ
                     IKKGEEITGSRIKVKVVKNKVAPPFKIAEFDIMYNEGISKEGDLINTGVKTGVIERSG
                     NSYAFGETKLGTGIDNAKEFLKQNPKIANEIDKAIRQKALKEGK"
     gene            complement(656..1687)
                     /gene="mltG"
                     /locus_tag="PL075_01345"
     CDS             complement(656..1687)
                     /gene="mltG"
                     /locus_tag="PL075_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOD96873.1"
                     /translation="MKQFLFLVLGIYLIGIIFYLAIFYYQLKPLPVEAGLQNQTVQFI
                     VQKGDSFKEIAERLEEQGLIRSSKSFKVYLLIHGWANKLQPGVYQITYGSDASTIAKT
                     LVAGMPAEKTIIIPEGYTLLDIEKKLKKEEVLGDGESLLTLKISDFQKDYDFLKNAPP
                     TATLEGFLFPDTYRFKINSPARLIAKRMLDNFQKILTPEIQTALQNTSLNFYQIITLA
                     SLIEGEIPLEEDRPVVSGILLKRLESQMPLQVDVTIVYIKCNILQVENCRQLSSADFK
                     IDSPYNTYQNLGLPPGPVNNPGQSAIKAALNPIENPYWYYLSDPKTGKTIFSKTLKEH
                     NAAKAQYLK"
     gene            complement(1832..2929)
                     /gene="rodA"
                     /locus_tag="PL075_01350"
     CDS             complement(1832..2929)
                     /gene="rodA"
                     /locus_tag="PL075_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOD96874.1"
                     /translation="MNKTWKQISFSLIAPIVLLSLFSLLTLYSLSLQDRDPLQIFKKQ
                     AFWFLFCFAVFYIIARFFDYRILNTQNFFVIGLYLFTLILLILVLLIGPNIKGTSGWL
                     NFKFFSFQPIELAKLALIILLARYFATWHIEIWRPRRLLATAVYTGLYALLAFLQPDM
                     GSVVFFVLIWLGMLLVSGLKFKHFVILLIVFVILSLVSWQYFLKPYQKERILTFLNPD
                     RDPLGSGYSVSQAKIAIGSAGLFGKGLGQGLLTQLRFLPEAKTDFFFAAFVEEWGVVG
                     ALTLFLIYGWLFFQIYHIGLKSENNFSRLFCFGYLLILIVQIFLNLGANLGFLPVTGL
                     PLPFLSYGGSNLLINFIALGIIQNIKNVHQK"
     gene            complement(2944..3759)
                     /locus_tag="PL075_01355"
     CDS             complement(2944..3759)
                     /locus_tag="PL075_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96875.1"
                     /translation="MADDFTQQIIRLEKIRKRKKIVAVLIPILIIVLIVLVKVFFVKP
                     APTCFDGIKNGNEEGIDCGGPCLACGIKYALPIEVVSTKILSETPNTSQVLIQIKNSN
                     AEYGVNFKYTATLYTAFGEELKTITEKDFILPFSSKYLLLQKIDIPAEEINRVEVNFD
                     YQQKDWFYSTKKTVELFSVLDKHIRLMGPQEAGFLEVQAQIKNNTNVDFSEVEIIILL
                     FSKTGEIINAAKTQTSDFAAYSSRTITYVWLYNFPDSNILDFYHIEVFADAYK"
     gene            complement(3780..4859)
                     /gene="mnmA"
                     /locus_tag="PL075_01360"
     CDS             complement(3780..4859)
                     /gene="mnmA"
                     /locus_tag="PL075_01360"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOD96876.1"
                     /translation="MIKNTSPKTVLVGLSGGLDSAITAKLLSMANFHVIGVYLRLWQE
                     SKKIIQQRETQVKKIARQIGIPLLLLDAQKDFQKYVVQYFLDELKSGATPNPCVECNY
                     YVKFRLLNNLAQALPADYIATGHYAKKVVVNQSLLPERKTKIFKIIKAKDKNKDQSYF
                     LWHLNQKLLQKIIFPLGDFTKEEVKIMAQKWRLNFKNITESQDLCFINDLDRFIQKLV
                     KPQPGPIINAISNQALGEHQGLAFYTIGQRKYIHIPAAEPYYVVKKDLRKNILFIAPA
                     SQIKHFQQQIITLKKVNFIRQKPKLPFECEVKLRYRSKPLKAKLYFHKFYYLQLQKPI
                     FGVTPGQSAVFYHRQELIGGGIIKK"
     gene            complement(4864..5826)
                     /locus_tag="PL075_01365"
     CDS             complement(4864..5826)
                     /locus_tag="PL075_01365"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96877.1"
                     /translation="MIYFLYGIDSYRINEKTKEITKQYRKKYPDLLGFEIFDLTEIDA
                     FQNLKNFFDNTSMFVQQKLAVAKDVFHSPEFSSLASFLKNSSLNQSQENNLILTGIFK
                     DEKEMNKLPKEKSDLFNFLLKHATKSQKFMPFKSFNEALPWLKKQLKEHQIKIDLPAL
                     KILFDNYGNNPYQLINELIKISLFQPNEKITIKEVNLLTRFPLHPDFFAIFRNFFEGN
                     KKQMLFNLEKSLKSGTDEGEIFNYFIKQVRAAIYLLNHQTKHIDLPPFVINQIKRHLT
                     RWPNAQQKLTLVYHQLSKIDRLVKRGAIDYQSALEILIAQLKFF"
     gene            complement(5823..6695)
                     /gene="mutM"
                     /locus_tag="PL075_01370"
     CDS             complement(5823..6695)
                     /gene="mutM"
                     /locus_tag="PL075_01370"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOD96878.1"
                     /translation="MPELPEVETIVNDLKKIVVGKKIIDVWVDTPKIVKRPDVKTFAR
                     QIKGLKIKNIYRRGKNIIFDLSNNYALLIHQKLTGHLLYGKWAIKGKKIVPLSDGRLS
                     EPVNNYIHLLLFLNNGYELALSDLRKFAKIIFDSTEKISNLKELQKLGPDPLMNNFTE
                     EELKKILQSKKGKIKQVLMDQTALAGIGNIYSDEILWWAQIHPLRSTRSLTDKEIHRL
                     YHYIKEVLATAIKARGASVSDYRDPKGEPGRYDQIRRVYRRENQKCYRCGTLIKRIKI
                     GSRSSCYCPNCQKL"
     gene            complement(6698..7009)
                     /locus_tag="PL075_01375"
     CDS             complement(6698..7009)
                     /locus_tag="PL075_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96879.1"
                     /translation="MPYIKKETREKLDPKIDELVEAIVAEAQKDDPKTTFVGILNYAF
                     TTTALKTAKKLFGQMRYWLIASLTGLFHNMADEFYRRLGAPYEEEQIKKNGDLDFYQK
                     F"
     gene            7174..7755
                     /locus_tag="PL075_01380"
     CDS             7174..7755
                     /locus_tag="PL075_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96880.1"
                     /translation="MNKQSKLVIVVIVALILIVGTIFFIIQINKPKAEAPQIITQIQT
                     ANENIVNLAKENETLLATIQEHYQKNQIKEALDSAVTLKDSIQKLTDTSLQLTEQLKE
                     AVVVLPDMSSDVRLVVEQAIQYEITAMNHLITYSAYKEILTQQIGIQYEAMIDGRSLE
                     QIPDINNIIKQMQDLLTLIQVNIQSARDLFQTI"
     gene            7766..9358
                     /gene="gpmI"
                     /locus_tag="PL075_01385"
     CDS             7766..9358
                     /gene="gpmI"
                     /locus_tag="PL075_01385"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOD96881.1"
                     /translation="MKKSNVLIILDGWGIGLRDDSNPIHRVKPPTIENFKKNYPMTSL
                     QAGGIAVGLPWGEAGNSEVGHLTMGIGKIVYQYYPRISLNIQNGEFFKNKALNNAYNF
                     ALKNNSTLHCLGLLSESHAHSAYDHIIALLQMAVKKQLSKICFHFFTDGRDSRPQSGK
                     SLVEHFLTDKKQFNIGTIGSLSGRYYALDRSKNWSLTERVYRLLTEGEGKTAKDPLEV
                     FDYYYNQKKLSDPYIEPTLITSNNNKDNWPLIREGDSVIFFDFREDNERQLVSAFVLD
                     DFDHFPRKKIKNLHVTTMTQYDAKFPVDVAYFPEKISTCLTDVISKRGLKQMKIAETE
                     KYYHLSYFFNGLTDKIFPDEFRILVPSKETPHPEEFPAMRAPEITERLIAAMNENIYD
                     FIAVNYANPDVIAHTGNYNAVMEAIKILDQQLAKIYEVAQKMGATLFVTADHGNAERL
                     YDPLTGEKDTYHNANPVPFYLVDESFRLKTPRTQEEIYDAESHTTGSLCDIAPTILEA
                     MSIEIPNEMTGESLFTNLISYL"
     gene            9425..11863
                     /gene="leuS"
                     /locus_tag="PL075_01390"
     CDS             9425..11863
                     /gene="leuS"
                     /locus_tag="PL075_01390"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOD96882.1"
                     /translation="MKFFKYPFQKIEKKWLTQWQKSNFKTYQAKDFPKKSKYYVLEMF
                     PYPSGEGLHVGHVENYTAGDIYARFKRMNGYEVFHPMGWDAFGLPAENYAIKIKKHPS
                     EIVKQNIRVFKKQLLRMGFGYDWKREINTTDPKYYRWTQWMFLKLFEKGLAYQKEAPI
                     NFCPSCKTGLANEEVTSEGTCDRCGTPVEKKLLKQWWIKITAYAERLLKDLDLLDWPA
                     DIKEMQKNWIGKSEGHQIKFTLKNNNNFKVGEIEVFTTRADTLFGATYLVLAPEHLVI
                     NQLKDYIENYYEVQHYIMDAMQKSDLERTDLAKVKTGVEIRGIKAVNPANQETIPIWI
                     SDYVLMHYGTGAIMAVPAHDERDFEFAKKFKLNIVEVISPDGKGHLLEEAYTGDGVLI
                     NSGPFNGLDSKSAQEKIALYVGAKKVTNYKMRDWIFSRQRYWGEPIPIIHCPKCGIVP
                     VPEKDLPVTLPNVKSYEPTGTGESPLSSIESWVNTKCPKCGGPAKRETNTMPQWAGSC
                     WYYLRYLDPKNNKKFVDLKKEKKWLPVDIYIGGKEHAVLHLLYARFWHKFLKDIGAIT
                     TEEPFTKLVNQGIILGSDSRKMSKSFGNVVNPDNMVKQYGADVLRMYIMFLGPLEDMK
                     AWNTEGINGLSRFISRVWNLYQNQLRMKGKEVKNMELEKTLHRTIKKVTDDTENLRFN
                     TAISALMECLNKMQEKEIKISKNYLKTFLKLLAPYAPFIAEELWHALGSRTTIHKERW
                     PKYNEKYLQKNTFELLVQINGKLRAKIKAPLNITQNQAEKLALAQPSVQKFIKNNKIK
                     KVIFVANKVINFVV"
     gene            11975..12544
                     /locus_tag="PL075_01395"
     CDS             11975..12544
                     /locus_tag="PL075_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96883.1"
                     /translation="MEQGDSSPSKTKDQTAKKNKNKKKLIIISFVCVVIILFGVSGAF
                     GLIKERIQRTIDTYKTQKAIEQFYQGLDEYYQKFAEDTYGGKTPQETLDMFIEALENG
                     DIDLAAKYFAMDDNLSRKKWEDGLKQAQQEGRIGQIVEELKRAQPSTSQPGYETAYEF
                     MILGENGLADYSVEMEFNQYSGVWKIKSM"
     gene            12634..17643
                     /locus_tag="PL075_01400"
     CDS             12634..17643
                     /locus_tag="PL075_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOD96884.1"
                     /translation="MFQKILSLLFGLGIIFFPLSALAYSNTTTHPALTSEMVKFYNNF
                     SDKKINDQQRQWMMDGSSKEDVPTTRTLNHFYDPIYQMGLSGEINAGELTPFIKVLEP
                     MILSAKEWAQNSYAQATFLAEAYKNAALNPFTQSTKSAVEILTTHTWEKAINSYINGN
                     QQDAFENLGHILHLIEDMAVPAHVRNDHHLRGDAYENWAAQFGPDNINVVSQLKNKSP
                     LKFNSLNQYFDYLAVYTNTHFYSQDTIGIQTGYNQPEWNYLNTKILDGYVYSINNDDF
                     GEYILVRKGLSNSYFVSTIFDLSVQDKLIYSDYWSHLSRQAVLTGSGVIDLFFQEVEK
                     YKNDLAFKEKINEAMIKTTLENIKKLFGNLFGDKQTETANSNFEEIANNANSMNNNSS
                     EQVNEINIPLTPTPQIESLNEIVINPTPFFTPSPTPTSKPTPTPSLSVTPLPSPTKTP
                     APTPTLTPRPTYSPSPSPTPLVTVISSGNSSKTINFCSISNGIPTSNKIIINEIAWMG
                     TENSANDEWIELKNISNESIDITNWQLIDKNNQIKISFPSKILAPGEFYLLERTDDAP
                     VVNIKADLTFTGVINDTDETLQLFDSHCILQDEAKTNENGQWPAGNKDEKRSMERDTD
                     LTWHTYSGEINNGIYGTPKQENSLKIIEPSPSPSPSPSETPSPSPSPSPSSTPTPTPT
                     LTSTAQILISEILYDAEESDEGKEFIELYNPNNFEVDLSHWSLRLIKNNETTSDSLGK
                     IETNENHIISPRGFLLIGYNKYSSSTYNIEADILRTSYTLPNPTQENIYTIRLLNKEG
                     ITVDEITYQENSCLTGASLERKAKETSTSISMTEGEDQFLGNGYDSDLMTDFIIRSPQ
                     PQNSSSLPEPREKPPAPENISYQFTNGGLMLYFSLSQPATSSALEFESFYSYFKEDLS
                     NNFGSSASAILSATIKITKADWMNKSFEGMIEGFPLDQLNYLYLRMRSKDKDGLVSDL
                     SEIIFVEPATEIINQAPIVFYSGKRDKEFPAQKFRLGEFYHFISEESGYLAQINALVL
                     TFNCSMFVKAEVYSVDNGEIKNLIGESKEPSLINVFWYPNYRGWTFESGQVILEKNKE
                     YAVRFLLDHLIVDGNCQMAFHQNEEENKMIMDILGYKTALNNLTQKTANHNPLPEEET
                     IFENTISFSAQIFSYNNWPVKLEVELRPRQESFDDQILKGDSHFFESSYLDSGKIAEI
                     TVGGLADGAYHWRARVIDIFGNPSDWYYYGDSQKYYRYNWESPPINIQDNSDFYVDAE
                     RTDYPENTLYYQPLAENINDLPTSYTIWYGPDYRIFTFSPHQSGYVDSAEVAAHYNHC
                     TFPMSAELYSLSPDQNDGLPDQLLAKSMDDIPHPISDYTQPYFESFKLDGWRFDREVY
                     LEAGHFYGLKIKFSDKHDWAGMANCGVQFGLRKDANNSRVYTMRGGRLYLDFDEDLRL
                     VIYKKDIAENEKATDSVLDSNNETEQNEMTNSEKTVDENQPLQNDNCENEAEAPLCST
                     SEIEINDQVENLDAELNTSIDNRQPSEEDSCFEENLLLPEEESVSPSENENILLPENI
                     APPVETTTSTELINSSDSFLNENTSSLENFSSSDQTVEEEEISEETSTSVENESEIEP
                     KAIITEEESDLFPDDSETTLMDLSDIEEKTEEENESEMMPDLSEVEEGEKESESGDGI
                     E"
     assembly_gap    17893..17992
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18331..18752
                     /gene="rnpB"
                     /locus_tag="PL075_01405"
     ncRNA           18331..18752
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PL075_01405"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            19098..19586
                     /gene="rpsF"
                     /locus_tag="PL075_01410"
     CDS             19098..19586
                     /gene="rpsF"
                     /locus_tag="PL075_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOD96885.1"
                     /translation="MDKKVYEITFVLSSRLIDAELKELLEKIKQGLEGLGAKILKQSE
                     FNKINLAYPIKKETQAYFGYFWFELEPEKISEIKDVFLFEKNILRYLIVTPPPKHQQK
                     TVKPKAQPRILSKEQISALEEFVPLAAEGIETSTTQESRELTEVEEEKLEEKLEEIQK
                     LA"
     gene            19623..20084
                     /locus_tag="PL075_01415"
     CDS             19623..20084
                     /locus_tag="PL075_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOD96886.1"
                     /translation="MNFNKVFILGNLTRDPELRTTPAGQSVASFGVATNRFWNDKNGQ
                     KQSEVEFHNVVAWGKLAETASRYLSKGKLVFVEGRLKTRSWTDQNGVRHSRTEIIAQN
                     FQLGPRGAGETITPTGANEGIDAEAPADLDNLPEDIPVISEDEISADDLPF"
     gene            20103..20300
                     /gene="rpsR"
                     /locus_tag="PL075_01420"
     CDS             20103..20300
                     /gene="rpsR"
                     /locus_tag="PL075_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOD96887.1"
                     /translation="MKQCFFCSQNVDEVDYKDVELLPRFLTLQSKIIPAKKSGLCRKH
                     QQRLTKAVKRARYLGLIKYQP"
     gene            20363..21757
                     /locus_tag="PL075_01425"
     CDS             20363..21757
                     /locus_tag="PL075_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOD96888.1"
                     /translation="MKQNTKLITIIIISGFIGAIVGSFVTHQISFKNSSLASENIITT
                     ATSTEITEDTLNPESNQSGSHFLANLWDDIKNLIPKWVELKPSEQPKTSASPTPIPTP
                     AFYNSQVAYEQRIIEVVQKSSPAVVSIIITKNVPIVERYYSNPLEELPPEFQQFFWPF
                     FQFEIPQYQERGTEKREVGGGTGFIISSDGLILTNKHVVSDENAEYTVFLTNGDKYQA
                     KILAQDPIDDIALIKIEANNLPTLPLGNSDGVQLGQTVIAIGNALSEFRNTVSVGVVS
                     GLARSVVASDQMGNVETIEDVIQTDAAINFGNSGGPLINLNGEVIGINTAIASGAENI
                     GFAIPINRASKDIRSYQTSGKISAPFIGIRYTIITPSLQKEKNLPVDYGALVSKGQNG
                     ESAVFPDSPAASAGIKEGDIILEINGQKITVDRSLSAIIKNYNAGEKIILKVLRDQQE
                     LNIEVVLADRPDNL"
     gene            21777..>23245
                     /locus_tag="PL075_01430"
     CDS             21777..>23245
                     /locus_tag="PL075_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOD96889.1"
                     /translation="MPFNRFTLKAQEALQSAQELAVSKNQGELKPVHLLYALVIQEGG
                     VVGMLLTRLGVSINSLVNALENELNRLPKLFVVNANVGQLYLSQDLIKVLDRATKEAA
                     DLKDEFISCEHLLLGIIEVASDLKPVLTKFGIQREAILRVMQEVRGSQHITDETPESK
                     FQVLEKYAVNLTQLAREQKLDPVIGREEELYRVIQILSRRTKNNPMLIGEPGVGKTAI
                     VEGLAQKIVNGEVPESLKNKEIISLDLPGMVAGTKFRGEFEDRLKAFIKEIKAGEGKY
                     ILFIDEIHTIVGAGAAEGAIDASNVLKPSLARGELHAIGATTLRDYQRYIEKDPALER
                     RFQPVYIEEPSIEEAIAILRGLKDKYESFHGLKISDSALVAAVNLSVRYIPDRFLPDK
                     AIDLIDEAAAARRLELDSIPPEVEELRRKILNLEIEKQALKKEKKAQMRLKKIEADLK
                     KAKEEFKKLNEKWSGSKKLIDELHNLRKKLEDLKKEKEIA"
     assembly_gap    23246..23345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23346..24041
                     /locus_tag="PL075_01435"
     CDS             <23346..24041
                     /locus_tag="PL075_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD96890.1"
                     /translation="PGYVGYEEGGQLTEIVKHRPYSLILFDEIEKAHPEVFNILLQIL
                     DNGRLTDAKGRTVNFKNTVIIMTSNVGSEYLEQMSSLGFKMGEESDKENPATQNLRDK
                     IRLALKERFRPEFLNRIDEIIIFNPLTPKDIEQIVEIQLSVLRKRLENKDIKIELNDD
                     VKKYLVQEGFSPEFGARELKRVIQRLILDPLAHAIVSGEINAGDTVKLTLSNNKIVFN
                     QTNITKQTRSKTK"
     gene            24038..24748
                     /locus_tag="PL075_01440"
     CDS             24038..24748
                     /locus_tag="PL075_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96891.1"
                     /translation="MRYFIVSFLLAELYLVSGFSYFWLIIAWLLAFYFLINQFQGLEE
                     WEDKYLYLAYPLALLFGLFLYFPKNFTWTNFTNQIFLIAGYFIFYFLTKLYRRNCYQP
                     DFFRIFGPIAVAPLAFLFFRSNLFQNFFWKELIVFLALFLLFEVYRHVFAKQKMSAAL
                     SLIPAFVLLEFSWILNFLPINFLSLVVIWIILFVLINEFWLLNCERQFNLKYFLPELL
                     FGLILIAVILLSSSWWML"
BASE COUNT         8215 a         4113 c         4567 g         7682 t
ORIGIN      
        1 atggagcaat tagttcgttc gggttcaatt gatattgttg tggttgattc agtagctgct
       61 ctgacacctc gagctgaaat tgagggcgaa atgggagcac agcatattgg gcttcaggcg
      121 cgtttaatgt ctcaggcctt aagaaaaatt acagcgattg cggcaaaaac taaaacagtg
      181 attatattta ttaatcaaat tcgtatgcag gtaggcatta tgtttggcaa tcctgaaaca
      241 acacccggtg gtcgggcttt aaaattttat tcttcagtta gaatagatat tcgtcgaaca
      301 gctcaaatca aaaaaggaga ggagattact ggttcacgca ttaaggttaa ggtagtaaaa
      361 aacaaagtag cgccaccatt taagatagcc gaatttgata ttatgtataa tgaaggcatt
      421 tctaaagaag gtgatttgat taacaccgga gtaaaaactg gtgttattga acgctcaggc
      481 aactcttatg cttttggcga aacaaaatta ggaacaggta ttgataatgc taaagaattt
      541 ttaaaacaga atccaaaaat tgctaatgaa attgataaag ccattagaca aaaagcctta
      601 aaagaaggaa agtaattaag aaattaataa aaaaatccgc ctgaggcgga tttttttatt
      661 ttaaatattg agctttggct gcattatgct ctttgagtgt tttggaaaaa attgttttac
      721 ctgttttagg atccgaaaga taataccaat aaggattttc aataggatta agtgcggctt
      781 taattgcgct ttgtccggga ttattaaccg gtcctggcgg taatcccaaa ttttgataag
      841 tattgtaagg ggaatcaatt ttaaaatcag cagatgaaag ttggcgacaa ttctcaactt
      901 gaagaatatt gcatttaata taaacaatag taacgtctac ctgtaaaggc atttggctct
      961 ctaatcgttt taataaaata ccagaaacga cagggcgatc ttcttccaag ggtatttcgc
     1021 cttcaatgag agaagcgagc gtaataattt gataaaaatt gagagacgta ttttggagcg
     1081 ctgtttgtat ttctggagtt aaaattttct gaaaattatc gagcatgcgt ttggcaatga
     1141 gacgagcggg ggagttaatt ttaaaacgat aggtatcagg gaataaaaat ccttcgagtg
     1201 ttgcagtggg aggcgcattt tttaaaaaat cgtaatcttt ttggaaatca gatattttta
     1261 aagtaagaag cgattcgccg tcgcctagta cctcctcctt ttttaatttt ttttcgatat
     1321 ccaaaagcgt atacccttct ggaataataa tggttttttc ggcgggcatg ccagccacta
     1381 gtgttttggc aatggtggag gcgtcggaac cgtaagtaat ttggtaaaca cccggttgta
     1441 atttattagc ccaaccatga atcaagagat agactttaaa actttttgaa gaacgaataa
     1501 gcccctgttc ttcaagtcgt tcggcgattt ctttaaaact atcccctttt tgaacaataa
     1561 attgaacagt ctggttttga agtcccgcct ccacgggcag cggttttaat tgataataaa
     1621 aaatcgccag ataaaaaata atgccgataa gataaattcc taaaactaaa aaaagaaatt
     1681 gtttcataat attttatttt taagttttta taaaattgaa tatcaaatat tttcaatctg
     1741 aaattcaaaa ttttttattg ctttactatg attatatgat ttgattaatt ctgatttgag
     1801 tcctatgttt tagtgtttta atagtgaaat attacttttg atgaacattc ttaatatttt
     1861 gaataatacc caaagcaata aaatttatca agagattaga cccgccataa cttaaaaacg
     1921 gcaaaggcaa acccgtcacg ggcaaaaaac ccagattagc ccctaaattt aaaaatattt
     1981 gaacaatcaa aatcaaaaga taaccgaagc aaaatagtcg ggaaaaatta ttttcacttt
     2041 ttaatccaat atgataaatt tggaaaaaca gccatccgta aataagaaaa agagttaaag
     2101 caccgacaac tccccattct tcaacgaagg ctgcaaaaaa gaagtcagtt ttggcttcag
     2161 gcaaaaagcg gagttgagtt aaaaggcctt gtcctaatcc tttgccaaac agccccgctg
     2221 atccaatggc aattttggct tgagaaacac tatacccgct gccgagcgga tctctatccg
     2281 gatttaaaaa ggtaagaatt cgctcttttt gataaggctt gagaaaatat tgccaactga
     2341 caagacttaa aattacaaaa acgattaaaa ggattacaaa atgcttaaat tttaagccag
     2401 aaacgaggag cattcctaac caaattagaa cgaaaaaaac tactgatccc atatcgggct
     2461 gaagaaaagc gagcaaggca tacaatccag tataaacagc agtagctaaa aggcggcgtg
     2521 gccgccaaat ttcaatatgc caagtagcaa aatagcgtgc taaaagaata attaaagcta
     2581 atttggccaa ttctatgggt tgaaaactaa aaaatttaaa attaagccag ccagaagttc
     2641 ctttaatatt ggggccaatg agtaaaacca aaatcagtag aattagagta aaaaggtata
     2701 gtccaatgac gaaaaaattt tgagtattta agatgcgata atcaaaaaaa cgagcgatga
     2761 tataaaatac agcaaagcaa aaaagaaacc agaatgcctg ttttttaaaa atttgcagcg
     2821 ggtctctgtc ttgtaaagaa agactatata aagttaaaag actaaaaaga gaaagtaaga
     2881 cgatcggagc aattaaagaa aaggatattt gtttccaagt tttgttcata aaagccgcaa
     2941 ttcttatttg tatgcatcag caaaaacttc gatatgataa aaatccaaaa tattagaatc
     3001 aggaaaatta taaagccaaa catacgtaat cgttcgactg ctataggcgg caaaatcaga
     3061 agtttgcgtt ttggccgcgt taataatttc gccggttttt gaaaaaagaa ggataataat
     3121 ttctacttca gaaaaatcca cattagtatt atttttaatt tgagcctgaa cttctaaaaa
     3181 tccagcctcc tgagggccca tgagccggat atgtttgtcc agtactgaaa agagctcaac
     3241 agtttttttc gttgaataga accagtcttt ttgctggtaa tcaaaattca cctcaacgcg
     3301 attaatttct tctgcaggaa tatcaatttt ttgaagcagc agatatttgc ttgaaaaagg
     3361 tagaataaaa tccttttcag tgatagtttt taattcttca ccaaaagcag tataaagagt
     3421 tgcggtatat ttaaaattaa ctccatattc agcattgcta tttttgattt gaataagcac
     3481 ttggcttgta ttaggggttt cagacaatat tttagtagaa actacctcta tcggtagggc
     3541 gtatttgata ccgcaagcaa gacaagggcc accgcaatca atgccttctt cgttgccatt
     3601 tttaattcca tcaaagcaag taggagccgg cttaacgaaa aaaactttca cgagcacaat
     3661 aagaacaata attaaaatag gaatcaaaac agcgactatt ttttttcgct tgcggatttt
     3721 ttctaaacgg ataatttgtt gagtaaaatc atcggccata tttctattat aactagaaat
     3781 tattttttaa tgataccccc gccgattaat tcttgacgat gataaaaaac cgctgattgg
     3841 ccaggcgtta ctccaaaaat tggtttctgc aattgaagat aataaaattt atggaagtaa
     3901 agtttggctt ttaatggttt gctgcgataa cgtagtttga cttcacattc aaaaggcagt
     3961 ttgggttttt gcctaataaa atttaccttt ttcaaagtta taatttgttg ttgaaaatgt
     4021 tttatttgag aagctggtgc aataaaaaga atattttttc taagatcttt tttgacgaca
     4081 taataaggct cggctgccgg gatatgaatg tattttcttt gtccgatagt gtagaaagcg
     4141 agcccctgat gttcgcctag cgcctgattg ctgatagcat taataattgg ccctggttgt
     4201 ggtttaacta atttttggat aaaacggtca agatcattta taaaacagag atcttgactc
     4261 tcagttatgt ttttaaaatt caagcgccac ttttgagcca ttatttttac ttcttcttta
     4321 gtaaaatcac cgaggggaaa aataattttt tggagcagtt tttgatttaa atgccaaaga
     4381 aaataggatt gatctttgtt tttatctttc gctttgataa ttttaaaaat ttttgttttt
     4441 ctttcgggca gcagcgattg attaaccacc actttttttg cataatggcc ggtggcaata
     4501 taatcagccg gcaatgcttg ggcaagatta ttgagaaggc gaaactttac ataataattg
     4561 cactcaacac aaggattggg cgtagcaccg ctttttaatt catcaaggaa atattgaacg
     4621 acatattttt gaaaatcctt ttgagcatct aacaataata aagggattcc aatctggcgc
     4681 gctatttttt taacttgcgt ttctctttgt tgaataattt ttttgctttc ctgccacaga
     4741 cggagataaa cgccaataac atgaaaatta gccattgata ataatttagc cgtgatggcg
     4801 ctgtcaaggc cgcctgataa gccaacaaga actgtttttg gtgaggtatt ttttatcatt
     4861 gaatcagaag aattttaatt gagcaataag aatttctaaa gcagattgat agtcaattgc
     4921 ccctcttttg actagccggt caattttact taactgatga taaacgagag ttaatttttg
     4981 ctgggcatta ggccagcgag tcaaatgacg tttgatttga ttaataacga aaggaggcaa
     5041 atcaatgtgt ttggtttgat gatttaataa ataaatagca gcccgcactt gtttaataaa
     5101 ataattgaaa atttctccct catcagttcc gcttttaaga gatttttcca gattaaagag
     5161 catttgcttt ttgttgcctt caaaaaaatt gcgaaagatg gcaaaaaaat ccggatgtag
     5221 aggaaaacga gttaagagat taacttcttt gatggtaatt ttttcattgg gttgaaacaa
     5281 agaaatcttt attaattcat taatgagttg atatgggtta ttgccgtaat tatcaaataa
     5341 aatttttaaa gcgggcagat caattttaat ttgatgctct tttaattgtt tttttaacca
     5401 cggcagagct tcattaaacg atttaaatgg cataaatttt tgacttttcg tggcatgttt
     5461 taataaaaaa ttaaacaaat cacttttttc ttttggcaat ttgttcattt ctttttcgtc
     5521 tttaaaaatg ccagtaagaa taagattatt ttcctggctt tgatttaaag aagaattttt
     5581 taaaaatgaa gcaagtgaag aaaattcagg gctgtgaaaa acgtctttgg ctacggctag
     5641 tttttgttgg acaaacatag aggtattgtc aaaaaaattt ttgagatttt gaaaagcatc
     5701 aatttcggtt aaatcaaaaa tttcaaaacc caaaagatca ggatatttct ttcgatattg
     5761 tttagtgatt tctttagttt tttcgttaat gcgataggaa tcaatcccgt agagaaaata
     5821 aatcataatt tttggcaatt aggacaataa caactagagc gactaccaat tttgattctt
     5881 ttaatcaaag taccgcaacg ataacatttt tggttttcgc gacgataaac acgtcggatt
     5941 tggtcatatc gtccgggttc gcctttggga tctcgatagt cactcaccga agcgccccgg
     6001 gccttaatag cagtagccag cacctcttta atataatggt agaggcgatg aatttcttta
     6061 tcggttaaag atcgagtaga gcgaagaggg tggatttgag cccaccacaa aatttcatct
     6121 gaataaatgt ttccaattcc agcaagcgca gtttgatcca ttaatacctg tttaattttg
     6181 ccttttttgc tttggagaat ttttttcagt tcttcttcgg taaaattatt cattaaaggg
     6241 tcgggtccca atttctgtaa ttcttttaga ttactgatct tttcagtaga atcaaaaata
     6301 attttagcaa attttcttaa atccgaaagc gctagttcat acccattgtt taaaaaaaga
     6361 agaagatgaa tgtagttgtt aacaggttca ctcaatcggc catcactcaa aggaacaatt
     6421 ttttttcctt tgatcgccca ttttccatac aaaagatgac cagtaagttt ttgatgaatg
     6481 agcagggcat aattattaga taaatcaaag ataatatttt tacctcggcg ataaatattt
     6541 ttaattttta atcctttaat ttgccgagca aaagttttaa catctgggcg ctttactatt
     6601 ttgggcgtgt caacccaaac atcaatgatt tttttgccaa ccactatttt ttttaaatcg
     6661 ttaacaatgg tttctacttc aggtaactct ggcataatta gaatttttga taaaaatcaa
     6721 gatcgccatt ctttttaatt tgttcctctt cataaggcgc gccgagacga cgataaaact
     6781 catcggccat attgtgaaaa agtcctgtta aagaggcgat gagccaataa cgcatttggc
     6841 caaacaattt tttggctgtt ttgagcgccg tagtggtaaa ggcgtaatta agaataccaa
     6901 caaaagttgt tttgggatca tctttttgcg cttcagcaac aattgcttct accaactcat
     6961 caatttttgg atctaatttt tcgcgagttt cttttttaat ataaggcata ctaatttttt
     7021 atttccgaca tctttagcat accgcaaaat gatggatttt tcaaacaaaa tcacgaccac
     7081 atagctataa tgtagagata ttcgctctct taataaaaag caatgaatac cccttttctt
     7141 ttttttcaaa ttttgataca ataaaaatac tttatgaata aacaatctaa acttgtcatt
     7201 gtggttattg ttgctcttat tttaattgtt ggcaccatct tttttattat tcaaattaac
     7261 aagccaaaag ccgaagctcc acagattatt actcaaattc aaacagctaa tgagaatatc
     7321 gttaatttgg caaaagaaaa cgaaacgctt ttagccacta ttcaagaaca ttatcaaaaa
     7381 aatcaaatta aggaagcgct tgattcagcg gtgacattaa aagattccat tcagaaatta
     7441 acagacacat ctcttcaatt aactgagcaa ttaaaggaag cggtggttgt tttgcctgat
     7501 atgagttcgg atgttcgttt ggttgtagaa caagctattc aatacgaaat taccgctatg
     7561 aaccatctca ttacttattc ggcttacaaa gaaatcctta cccaacaaat tggcattcaa
     7621 tatgaagcga tgatagatgg ccgttcttta gaacagattc ccgatattaa taatattatt
     7681 aaacagatgc aggatctttt gacattaatt caagtcaata ttcaatcggc tcgagatcta
     7741 ttccagacaa tttaaataaa tacttatgaa gaaaagcaat gttctgatta ttttggacgg
     7801 atggggcatt ggtttgcgtg atgattccaa tcccattcat cgcgttaaac caccaacaat
     7861 cgaaaatttt aaaaaaaatt atccgatgac cagcttgcaa gcaggcggta ttgctgttgg
     7921 cttgccatgg ggcgaggcgg gcaatagtga agtgggccat ttgacaatgg gtattggaaa
     7981 aattgtttat caatattatc cgcgtattag tttaaatatt caaaatggag aattttttaa
     8041 aaacaaagct ttaaataacg cctataattt tgcgctaaaa aataattcta ctctacactg
     8101 cctcggttta ttgtcagaaa gtcatgccca tagcgcttat gatcatatta ttgctctttt
     8161 acagatggct gttaaaaagc aactatcaaa aatatgcttt cactttttta ctgatggccg
     8221 tgacagccgt cctcagtcag gcaaatcatt agtggagcac tttttaacgg ataaaaaaca
     8281 atttaacatc ggaacaatag gtagtttatc aggtcgttac tatgcattgg atcgttctaa
     8341 aaattggagt ttaacagagc gcgtttatcg tttattaacc gaaggcgagg gcaaaacagc
     8401 taaagatcct ttggaggttt ttgattacta ctataatcaa aaaaaattaa gcgatcctta
     8461 tattgagcca actttaatca cttcaaataa caataaagat aattggccgc tcattcgcga
     8521 aggcgacagc gtcatttttt ttgatttccg cgaagataat gagcgacaat tagtaagcgc
     8581 ttttgtttta gacgattttg atcattttcc acgtaaaaag ataaaaaatt tacatgttac
     8641 tacaatgact caatatgatg ctaaatttcc tgttgatgtt gcttattttc cagaaaaaat
     8701 ttctacttgt ttgacagatg ttattagcaa aagaggattg aaacaaatga aaattgctga
     8761 aacggaaaaa tattaccacc tcagctactt ttttaacgga ctaactgata aaatttttcc
     8821 tgacgaattt cgcattcttg tgccctccaa agaaacgccg cacccagaag aatttccagc
     8881 catgcgcgct ccagaaatca ccgaacgatt gattgcggca atgaatgaaa acatctatga
     8941 ttttattgct gttaattacg ctaatcccga cgtcattgct catactggta attacaatgc
     9001 cgtaatggaa gctattaaaa ttttagatca gcaacttgca aaaatttatg aagtagctca
     9061 aaaaatggga gccactttat ttgtaacggc tgatcatggc aatgccgaaa gattatacga
     9121 tcctttaacc ggcgaaaaag acacctatca caatgctaat cctgttccgt tttatttagt
     9181 ggatgaaagt tttcgtttga aaacaccgcg tacccaagaa gaaatttatg atgctgaaag
     9241 tcataccacg ggctccttgt gcgatatcgc cccaactatt ttagaagcaa tgagtattga
     9301 aattcctaat gaaatgacgg gcgaaagtct tttcaccaac ttgatttcgt atttataaat
     9361 tttatacttt tattaattat ttccagcgta attaaacttc aaaattaata ttactgtaaa
     9421 acttatgaaa ttttttaaat acccttttca aaaaatagaa aaaaaatggt tgactcaatg
     9481 gcaaaaatcc aattttaaaa cttatcaagc caaagatttt ccgaaaaaat caaaatatta
     9541 tgtgttagag atgtttcctt atccttcggg cgaagggctt catgttggtc atgtggaaaa
     9601 ttataccgct ggtgatattt acgcgcgttt taagagaatg aatggttatg aagtgtttca
     9661 tccaatggga tgggatgctt ttggtcttcc ggcagaaaat tatgcaatca aaattaaaaa
     9721 acatccgtcc gaaattgtta aacagaacat ccgagttttt aaaaaacaat tattacgcat
     9781 gggttttggt tatgattgga aaagagagat taatactaca gatcccaaat attatcgctg
     9841 gacacaatgg atgtttttaa aactctttga aaaaggatta gcttatcaaa aagaagcgcc
     9901 cattaatttt tgtccttctt gtaaaacagg attagcgaac gaagaagtta cgagtgaggg
     9961 tacctgtgat cgttgcggca cgcccgtgga gaaaaaatta ttaaagcaat ggtggattaa
    10021 aattaccgct tatgcagaaa gattattaaa ggatttggat ttattggatt ggccggcaga
    10081 tattaaagaa atgcaaaaaa attggattgg caagtcagaa ggtcatcaaa tcaaatttac
    10141 tttaaaaaat aataacaatt ttaaggttgg agaaatagaa gtttttacga cgcgagctga
    10201 tactttattt ggcgctactt atttagtact ggcgccagag catttagtaa tcaatcagtt
    10261 aaaggattac atcgaaaatt attatgaggt acagcattat ataatggatg ccatgcaaaa
    10321 atcagatttg gaacgtacgg atttagctaa agttaaaaca ggcgtggaaa tccgtggtat
    10381 taaagccgtt aatccagcca atcaagaaac aattccgatt tggatttcgg attatgtcct
    10441 aatgcattat ggaacgggcg ctattatggc cgtgccggcg cacgatgaac gtgattttga
    10501 attcgctaaa aaattcaaat taaatattgt tgaggtaatt agtccggatg gaaaaggaca
    10561 tttattggaa gaggcctata ctggcgatgg agtattaatt aattcaggac catttaatgg
    10621 tcttgatagt aaaagcgctc aagaaaaaat tgctttatac gtaggcgcaa aaaaggtaac
    10681 aaattataaa atgcgtgatt ggattttttc tcggcagcgc tactggggtg aacctattcc
    10741 tattattcat tgtcccaaat gcggcattgt gcctgtgccg gaaaaagatt taccggtgac
    10801 tttgcccaat gttaaatctt atgagcctac aggcactggt gaatcgccac tttcttctat
    10861 tgaaagctgg gtcaatacta aatgtccgaa atgcggcggt ccagccaaaa gagaaaccaa
    10921 tacgatgccg caatgggcag gttcctgctg gtattattta cgctatcttg atccaaaaaa
    10981 caacaaaaaa tttgttgatc taaaaaaaga aaaaaaatgg ctgccggttg acatttatat
    11041 tggcggcaag gagcacgctg ttcttcactt actctatgcg cgtttctggc ataaattctt
    11101 aaaagacatt ggggcaatca caaccgaaga accatttaca aaattagtaa accaaggaat
    11161 tattttaggt tcggatagcc gaaaaatgtc taaatcattt ggcaatgtgg taaatcctga
    11221 taatatggta aagcaatatg gcgccgatgt tttaagaatg tacattatgt ttctaggacc
    11281 gcttgaagat atgaaggcct ggaatacaga gggcatcaat ggattgtcgc gttttatcag
    11341 ccgcgtgtgg aatttgtatc aaaatcaact gcgcatgaaa ggcaaagaag taaaaaacat
    11401 ggagctggaa aaaactctgc atcgtacaat taaaaaagtt actgatgaca cagaaaattt
    11461 gcgatttaat accgctatta gcgcgttaat ggaatgcctg aataaaatgc aagaaaaaga
    11521 gattaaaatt tctaaaaact atttgaaaac atttttaaaa ttacttgcgc cttatgctcc
    11581 ttttattgct gaagaattat ggcacgcatt aggtagtcgc actacaattc acaaagaaag
    11641 atggccgaaa tacaatgaaa aatatttgca aaaaaatact tttgaattgc ttgtgcagat
    11701 taatggcaaa ctgagagcca aaataaaagc gccgttaaac attactcaaa atcaagcgga
    11761 aaaattggct ttagcgcagc cgtcagtcca gaaatttatc aaaaataata aaattaagaa
    11821 agtgattttt gttgccaata aagtaattaa ctttgttgtt tagtttatct tagtttttaa
    11881 aagcaatcta actttttaag gatattttta attaaaaagt tagatttttt aaaaaattaa
    11941 aattaatagg tcgctttaat aaaaattaat ttttatggag caaggagatt cttcgccatc
    12001 aaagacgaaa gatcaaacgg ctaaaaaaaa taaaaacaaa aagaaactaa taattattag
    12061 ttttgtgtgc gtcgttatta tactttttgg cgtcagtggc gcctttggat taattaaaga
    12121 aagaatccaa agaaccattg atacttataa aactcaaaaa gccattgaac aattctatca
    12181 aggattagat gaatattatc aaaaatttgc cgaagatact tatggcggca aaacacctca
    12241 ggaaacacta gatatgttta ttgaagcact ggagaatgga gacattgatt tagcagctaa
    12301 atattttgct atggatgata atttaagtag aaaaaagtgg gaagatggat taaaacaagc
    12361 tcaacaagaa ggtagaatag gccaaattgt agaagaatta aagagagctc aaccatcaac
    12421 ttctcaacct ggttatgaaa cagcttatga gtttatgata ttaggcgaga acggattagc
    12481 cgattattct gttgaaatgg aattcaatca atattctggt gtttggaaaa taaaaagtat
    12541 gtaaattttt aatagcaatc tatttttaga ttgctatttt tctatctttt aagatttaat
    12601 tttttaagtc gtttttttga aataatcaaa aatatgtttc aaaaaatttt atcacttctt
    12661 tttgggttgg gcattatctt ttttccattg agtgcccttg cttatagcaa taccacgact
    12721 catccggctt taacttcgga aatggttaaa ttctataata atttttctga taaaaaaata
    12781 aatgatcaac aaagacaatg gatgatggat ggcagtagca aagaagatgt gccgacaacg
    12841 cgcacattaa atcattttta tgatcccatt tatcaaatgg gattatccgg tgaaattaat
    12901 gccggagaat taacgccttt tattaaagtt ttggagccaa tgattttgtc agccaaagaa
    12961 tgggcgcaaa attcttacgc ccaagctact tttttagctg aagcgtataa aaatgctgcg
    13021 ctcaatcctt ttactcagtc taccaaaagt gccgtagaaa ttttaacaac tcatacttgg
    13081 gaaaaagcca taaacagtta tattaatggt aatcagcaag atgcttttga aaatttagga
    13141 catattcttc atttaattga agatatggct gttccagctc acgtgcgaaa tgatcatcat
    13201 ttaagaggtg atgcttatga aaattgggct gctcaatttg gaccagacaa tattaatgtt
    13261 gtttctcaat taaaaaacaa gagcccatta aaatttaatt ccttaaatca atattttgat
    13321 tatctagctg tttatactaa cacccatttt tatagccaag ataccattgg tattcagact
    13381 ggttataatc aacctgaatg gaattattta aacacgaaaa tattagatgg atatgtatat
    13441 tctataaaca atgatgattt tggggaatat atacttgttc gtaagggact atcaaacagc
    13501 tattttgtta gcacaatttt cgatttatca gtgcaggaca aattaattta ttccgattac
    13561 tggtcacatt tatcgcgaca agcggtttta acaggttctg gtgttattga tttgtttttc
    13621 caagaagtag aaaaatataa aaatgattta gcatttaaag aaaaaattaa tgaagcaatg
    13681 attaaaacaa ccttagaaaa tattaaaaaa ctttttggaa atttatttgg cgataagcaa
    13741 acagaaacag ccaattctaa ttttgaggaa attgctaaca atgcaaattc aatgaataac
    13801 aacagcagcg agcaagttaa tgagattaat atacccctta ctcctacccc tcaaatagaa
    13861 tcgcttaatg aaattgttat caaccccacc ccttttttta ctccatcgcc aacgcccacg
    13921 tccaagccta cgccaacgcc atcattgtca gtaacgcctt tgccatcacc aactaaaacg
    13981 cccgccccaa cgcctacgtt aacaccaagg cctacgtatt cgccatcgcc atcacccacg
    14041 cctttagtaa ctgtgatttc ttccggcaat tcttcaaaaa caattaattt ttgctcaatt
    14101 tccaatggca ttcctacaag caataaaatt atcattaatg aaattgcttg gatggggaca
    14161 gaaaattcag ctaatgacga atggatagaa ttgaaaaata tttctaacga atcaatcgat
    14221 attaccaact ggcaattaat tgacaaaaac aatcaaatta aaatttcttt tcccagtaaa
    14281 attttagcgc cgggtgaatt ttatctttta gaaagaactg acgatgctcc agtagtcaat
    14341 ataaaagccg atttaacttt taccggtgta ataaatgata ctgatgaaac tttgcagctt
    14401 tttgatagtc attgtatttt acaagatgaa gcaaaaacaa atgaaaatgg tcaatggccg
    14461 gctggtaata aagatgaaaa acgatcaatg gaacgagata cagatttaac ctggcatact
    14521 tatagtggag aaattaataa tggaatatac ggcacgccca aacaagaaaa tagtttaaaa
    14581 ataattgagc cttcaccatc gccttctcct tcgccttcag aaacgccatc gccttctcca
    14641 tctccatcgc cttcgtcaac accaacaccc acgcccacac taacgtcaac agctcaaatt
    14701 ttaatttccg aaattcttta tgatgccgaa gaatcagatg aaggcaagga atttattgag
    14761 ttatataatc ccaataattt tgaggtagat ctttctcatt ggtcgttaag gcttataaaa
    14821 aataacgaaa caacgagtga ttctttggga aaaatcgaga ctaacgaaaa tcatataatt
    14881 tcaccgcgtg gatttctttt gattggttat aacaaatata gtagttccac ttataatatt
    14941 gaagccgaca tactccgcac ctcttacact ctacccaatc caactcaaga aaatatttat
    15001 accattcgtt tattgaacaa agagggcatt actgtcgatg aaataacata tcaagaaaat
    15061 agttgtctca caggagccag cttagaaaga aaagctaaag aaacttctac ttcaatttcc
    15121 atgaccgaag gtgaagatca atttttaggc aatggttatg attctgattt aatgaccgat
    15181 tttattattc gttcgcctca gccgcagaat tcttctagtt tgcctgagcc acgcgaaaaa
    15241 ccacccgctc ccgaaaatat ttcttatcag tttactaacg gtggtcttat gctttatttt
    15301 tctttaagtc aacccgccac atcttcggct cttgagtttg aaagttttta ttcttatttt
    15361 aaagaagatt tatccaataa ttttggatct tctgcttctg ccattttgtc ggctactatc
    15421 aagattacta aagctgattg gatgaataaa tcgtttgaag gaatgattga aggattcccc
    15481 ctagatcaat taaattattt atatttgcga atgcgaagca aagataaaga tggattagtt
    15541 tcagatttat cggaaattat ttttgttgaa ccggccacag aaatcatcaa tcaagcgcca
    15601 atagtttttt acagcggcaa aagggataaa gaatttccag cgcaaaaatt tcgtttaggc
    15661 gaattttatc attttatttc agaagaatca ggatatttag ctcaaattaa tgcactggtt
    15721 ttaactttta attgttcaat gtttgtcaaa gcagaagttt attccgttga taatggagag
    15781 attaaaaatt tgataggcga atcaaaagaa ccatcgctaa taaatgtttt ttggtatccc
    15841 aattatcgcg gttggacatt tgaaagcggt caagtgatat tagaaaaaaa caaagaatat
    15901 gctgtgcgat ttttgctgga tcatctcatt gttgacggta attgtcagat ggcttttcat
    15961 cagaacgaag aagaaaataa aatgataatg gatattttgg gttataaaac tgctcttaat
    16021 aatttaaccc agaaaacagc gaatcataat ccgttgcccg aagaagaaac aatatttgaa
    16081 aatacaattt ctttttctgc tcagattttt agctacaaca attggccagt gaaattggag
    16141 gtagaattga gaccacggca agaaagtttt gatgatcaaa ttcttaaagg cgattcgcat
    16201 ttttttgaaa gttcctattt agattctggt aagatagcgg aaataacggt tggtggcttg
    16261 gcggatggag cgtatcattg gcgagcgcgg gtgattgata tttttggcaa tccttctgat
    16321 tggtattatt acggcgattc gcaaaaatat tatcgataca attgggaatc accgccaatt
    16381 aatattcaag ataattccga tttttatgtt gatgctgagc gaaccgatta tcctgaaaat
    16441 accctttatt atcagcctct agcagagaat attaatgatc tgccgacatc gtatactatt
    16501 tggtatggcc ctgattatag aatattcact ttttcgcctc atcaaagtgg ttatgttgat
    16561 tcagctgaag tagcagcaca ttacaatcat tgcacgtttc ccatgagcgc ggaactttat
    16621 tctttatctc ctgaccaaaa tgatggcttg ccggatcagc ttttggccaa atcaatggat
    16681 gacattccgc accccatctc tgattataca cagccatatt ttgaatcatt caaacttgat
    16741 ggttggcgtt ttgatcgaga agtttattta gaagccggcc atttttatgg tttgaaaatt
    16801 aaattttccg ataagcatga ttgggccgga atggcgaatt gtggagtgca atttggtctt
    16861 cgtaaggatg cgaataattc ccgagtttat actatgcgag gtggtcgatt atatcttgat
    16921 tttgatgagg atctgagatt ggtgatttat aaaaaagaca tagcggaaaa cgaaaaagca
    16981 actgattctg tgctagatag caataatgaa acagagcaaa atgaaatgac taattcagaa
    17041 aaaacagttg atgaaaatca gcctcttcaa aatgataatt gtgaaaatga ggctgaggcg
    17101 cctttgtgca gtacatcaga aattgagatc aatgaccaag ttgaaaattt ggatgctgag
    17161 ctaaacacat caattgataa tcgtcagcca tcagaagagg attcttgttt tgaggaaaac
    17221 cttttgttgc cagaagagga aagtgtttca ccatcagaaa atgaaaatat tttattgcca
    17281 gaaaatattg cgccaccagt tgaaacaaca acttcaactg aattaataaa ttcttctgat
    17341 tcttttctca atgaaaatac ctcgtcatta gaaaattttt cgtcgtcaga tcaaacagta
    17401 gaggaagaag aaatatcaga ggaaacatca acgagtgtag aaaacgaaag tgagatagag
    17461 ccgaaggcga taataacaga ggaagaatca gatttatttc cggatgatag tgaaacgacc
    17521 ctaatggatc tatctgatat tgaggaaaaa acagaagagg aaaatgaaag tgaaatgatg
    17581 ccggatttat ctgaagttga agaaggagaa aaagaaagcg aaagtggaga tggaatagaa
    17641 tagactaatg agcaggcggc gccgctttaa gcggcgccgc cttcttcttt cgtctctaac
    17701 ttccgctgtt atgctttagc ataacagcac cgtctgtccc ttccgtttct aatttgcgct
    17761 attatgtttt aacttctgct gttatgcttt agcataacag caccatgcca cttgtgcctt
    17821 ttgtttctaa tttatggtgt tatgctttag cataacagca ccgtctgtcc cttccgtttc
    17881 taatttgcgc tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttaacttc
    18001 tggtgttatg ctttagcata acagcgctgc ctgctctctc tgaactttat tccaaatctc
    18061 ttcctgaact tcattctaaa tctctctccg agtattttat ctattatttt taaaaaatat
    18121 tttatttacc attttttaaa attaagcaag tgaagtagag gcagccgtga ttttaaaaat
    18181 aaatgcaatt gattagctca tgagttagct catgagctaa ctcatgagct acgaaaaagc
    18241 cttttatttt tgaaaattat tcatttctca tttaaatttt tctaatccct tgatttttgt
    18301 aaaaaaattt ctaaaattcc ttttagtttc tttggtaagt aggcaactgc tattaatatt
    18361 ttgaaacggc tttcgccaaa tcaaatatta atagaggaaa gtccgaacac tctattgttt
    18421 taaacttctt ttatgcagtt tagaacaatg gcgcgggtag ttgctaacag caaccggctg
    18481 gcattagcta gtcagggaaa gtgtcacaga gactataccg ctctgacttc tgtcggagta
    18541 agggtgaaaa gagtggaggt aagagctccc agctttgatt ggcgacaatc aaaggtgaaa
    18601 aaccctaccc agtgcaagat cgaacagtcg cctaaagcga caggtggatc gctctgaact
    18661 gaatggcaac attcagtcaa gataaatagt tgccagtcat tgaaatgcaa gtttcaatga
    18721 ccacagaatt cggcttattc ttatcaaagg aacaaaaact atcagaaaat cctgcccacc
    18781 ggcagggttt tctgattttt ttttgctttt tagtttgttt aggaaaagag gcgggcggcg
    18841 gaaaattttc aaagaaaatt ttccgccgcc gccccaccgt tttccattat tttatcaatt
    18901 gattgttgaa taaacagcaa aaatcacccg gatactggta actcaaagaa atttgattta
    18961 tactcaaagc agtatttcac cctttcgcgc taaaaactaa aaataaaatt ccaaagatca
    19021 aattttagct gttttaacat ttcaaggcac atgcaattag ttgacaattg aaaaattagc
    19081 agtaaacttt aattattatg gacaaaaaag tctacgaaat tacttttgtt ctaagcagcc
    19141 gattgataga tgcagaacta aaagaattgt tagagaaaat caagcaaggg cttgaaggat
    19201 tgggcgctaa aattttaaaa caatcagaat ttaataaaat taatctggcc tatcccatta
    19261 aaaaagaaac ccaagcgtac tttggttatt tttggtttga attagagccg gaaaaaattt
    19321 ccgaaatcaa agatgttttt ctatttgaaa aaaatattct ccgctattta attgttacgc
    19381 cgcctccaaa acatcaacaa aaaacagtta aacctaaagc tcagccccga atcttgtcta
    19441 aagagcagat tagcgcctta gaagagtttg ttcctcttgc tgcggaaggt atagaaacta
    19501 gcaccactca agaaagtaga gaattaactg aagttgaaga agaaaaatta gaagaaaaat
    19561 tagaagaaat tcaaaaatta gcttaaaaac aaaaaaaggt cttaaattaa ttttttgtaa
    19621 gtatgaattt taataaagta tttattcttg gcaatttaac acgtgatcca gaactgcgca
    19681 ctacgcccgc cggtcagagt gtggcctctt ttggcgtagc tacaaatcgt ttttggaatg
    19741 acaaaaatgg gcaaaaacaa agtgaagtag aatttcataa tgttgtggcg tgggggaaat
    19801 tagctgaaac agccagccgt tatttgtcca aaggtaaatt agtttttgtt gaaggccgat
    19861 taaaaacacg cagttggact gatcaaaatg gtgttcgtca ttcgcgaacg gaaattattg
    19921 ctcagaattt ccaacttggt ccgcgcggag ctggcgaaac tattactccg actggcgcga
    19981 atgaaggcat tgatgccgaa gctccagctg atttagacaa tcttcccgaa gatatccccg
    20041 ttattagcga agatgaaatt tcagccgacg atctcccatt ctaataatta attatttaat
    20101 ctatgaaaca atgttttttt tgcagtcaaa acgtagatga ggtggattat aaagatgtgg
    20161 aattattgcc gcgtttttta acgcttcaat caaaaattat tccagcaaaa aaatctggtt
    20221 tatgtcgcaa gcatcagcag cgattaacca aagcagttaa acgagcacgc tatttgggat
    20281 taatcaaata ccagccctaa tttaactttt tgatattttt tacgtataat ctaatagcca
    20341 tcaattaaca ttgttggatt ttatgaaaca aaatacaaaa ttaattacta tcattattat
    20401 tagcggtttt atcggcgcca tcgtcggcag ttttgtgact catcaaattt cttttaagaa
    20461 ttcttctctt gcttcagaaa atattataac aacagcaact tcaaccgaaa taaccgaaga
    20521 tacattaaat cccgagagca atcagtccgg ttctcatttt ctggcaaatt tgtgggatga
    20581 tattaaaaat ttgattccta aatgggttga gttaaaacca tcagaacaac ctaagacgag
    20641 cgcttcgcca acgcctattc ccactccagc tttctataat tcccaagtag cctatgaaca
    20701 aagaattatt gaagtggttc aaaaatcatc gcccgcagtt gtcagcatta tcattactaa
    20761 aaacgttccg attgttgaaa gatattactc aaatccttta gaagaattac cacctgagtt
    20821 tcagcaattc ttctggccat tttttcaatt tgaaattcct cagtatcaag agaggggaac
    20881 agaaaaaaga gaggtgggcg gcggcacagg gtttattatt tcttctgatg gcttaatttt
    20941 aacaaacaag cacgttgtga gtgatgaaaa tgccgagtat actgtttttc tgactaatgg
    21001 agataagtat caagccaaaa ttttagcgca agatcctata gatgatattg ctttaattaa
    21061 aattgaagcc aataatttgc ctacgcttcc tttgggaaat tccgacggcg ttcaattagg
    21121 ccaaacagtt attgccattg gcaatgccct gagtgaattt cgcaatacgg tttcagtggg
    21181 tgttgtttct ggtttagcgc gttcggttgt ggccagcgat caaatgggca atgttgaaac
    21241 cattgaagat gttattcaaa cagatgcggc tattaatttt ggcaattccg gcggtccatt
    21301 aattaatttg aatggtgaag tgattggaat taatacagct attgcttcgg gtgctgaaaa
    21361 tatcggtttt gctataccga ttaatcgcgc cagcaaagat atccgttcct atcaaacatc
    21421 agggaagatt tccgcgcctt tcattggtat tcgctataca ataattactc ctagtctgca
    21481 aaaagaaaaa aatctgccgg ttgattatgg cgcgttggtg agtaagggtc aaaatgggga
    21541 atcagcggtt ttccctgatt ctcccgcggc cagcgctggc attaaagaag gcgatattat
    21601 tttagaaatt aatggtcaaa aaattaccgt tgatcgttct ctttctgcta ttatcaaaaa
    21661 ttacaatgct ggcgaaaaaa ttatcttaaa agttttacga gatcagcaag aattaaacat
    21721 tgaagtcgtt ttggccgatc ggccagataa tctctaaaaa taattaatta aaaaatatgc
    21781 catttaatcg ttttacttta aaagctcaag aagcactcca atccgctcaa gaattagccg
    21841 tgagtaaaaa tcagggcgaa ttaaagccgg ttcatttgct ttatgcgtta gtgattcaag
    21901 aaggaggggt ggtgggaatg cttctaactc gtctcggagt aagcattaat tctttagtca
    21961 atgctttaga aaacgaactc aaccgtttac caaagctttt tgtagtcaat gctaatgttg
    22021 gccagcttta tttatcgcaa gatcttatta aagttttaga tcgagcgacc aaagaagcag
    22081 ctgatttaaa agatgaattt atttcttgtg aacacctgct tttgggtatt attgaagttg
    22141 cttcagattt aaaaccggtt ctaactaaat ttggtattca gcgcgaagct attttgaggg
    22201 ttatgcaaga agtgcgaggc agtcagcata ttaccgatga aacgccggaa agcaaatttc
    22261 aagttttaga aaaatatgca gtgaatttaa cccaactggc gcgggagcaa aaactggatc
    22321 ctgttattgg ccgagaagaa gaactttatc gtgttattca aattctttca cgccgaacca
    22381 aaaataatcc tatgctcatt ggcgagcccg gcgtcggcaa gactgctatc gttgaaggcc
    22441 tcgctcaaaa aattgtaaat ggtgaagtgc cggaaagttt aaaaaataaa gaaataattt
    22501 ctttggattt gccgggtatg gtagcgggaa caaaattccg tggtgaattt gaagatcgcc
    22561 tcaaggcttt tattaaagaa ataaaagcag gagaaggaaa atacattttg tttattgatg
    22621 aaatccatac tattgtaggt gcgggcgcag ccgaaggcgc aatagatgct tcaaatgttt
    22681 tgaagccatc tttggcgcgc ggcgaattac atgccattgg cgccactaca ctcagagatt
    22741 atcagcggta tattgaaaaa gatccggctt tggaaagacg tttccagcca gtttatattg
    22801 aagaaccttc aattgaagag gcgattgcca ttttgcgcgg tctgaaagac aaatacgaaa
    22861 gttttcatgg tttaaaaatt tctgatagcg cgcttgtcgc cgctgttaat ctttccgtgc
    22921 gctatatccc agatcgcttt ttgccggaca aagccattga tttaattgac gaagccgcag
    22981 ccgctcgtcg tttggaactt gatagtattc cgccagaagt ggaggagttg cgtcgcaaaa
    23041 ttcttaattt agaaattgaa aagcaagctc taaaaaaaga aaagaaagct cagatgcgtt
    23101 taaagaaaat agaagctgac ctgaaaaaag ctaaggaaga attcaaaaaa ttaaatgaga
    23161 aatggagtgg aagcaaaaaa ttaattgacg aacttcataa tttaagaaaa aaactggaag
    23221 atcttaaaaa agaaaaagaa attgcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnncccgg ctatgttggg tatgaagaag gcggccaatt aaccgaaatc gtaaaacatc
    23401 ggccgtatag ccttatttta tttgatgaaa ttgaaaaagc tcatcctgaa gtttttaata
    23461 tcctccttca gattttagat aacggtcggc tgactgatgc aaaaggacgg acagtaaatt
    23521 ttaaaaacac cgttattatt atgacttcca atgtaggtag tgaatatctg gagcagatgt
    23581 cttctttagg atttaaaatg ggcgaagaaa gcgacaaaga aaatccagcc acgcaaaatc
    23641 tgagagataa aattcgcctc gcgcttaaag agcgcttccg ccccgaattt ttgaatcgta
    23701 ttgatgagat tatcatcttt aatcctttaa cgcctaaaga tattgaacaa attgtagaaa
    23761 ttcaactcag cgtcctcaga aagcgattag agaataaaga tattaaaata gagctcaatg
    23821 atgatgtaaa aaaatacttg gttcaagaag gttttagtcc ggaatttgga gcgcgggaat
    23881 taaaacgagt aattcaaaga ttgattttag atccattagc ccatgcaata gtaagcggtg
    23941 aaatcaacgc gggcgataca gtaaaattaa cgctcagcaa taataaaatt gttttcaatc
    24001 aaacaaacat aactaaacaa acgcgaagta aaacaaaatg agatatttta ttgttagttt
    24061 tcttttagcc gaactatatc ttgttagtgg cttcagctat ttttggctga ttattgcttg
    24121 gcttttagct ttttattttt taattaatca atttcaagga ttggaagaat gggaggataa
    24181 atatctttat ttagcttatc cattggcttt gctttttggc ctctttctct attttcctaa
    24241 aaactttacg tggaccaatt ttaccaatca aatttttctg attgctggtt attttatttt
    24301 ttattttttg acgaaactgt atcggcgtaa ctgctatcag ccggactttt ttcgaatttt
    24361 tggacctatc gcggtagcac ctttagcatt tctctttttc cgttccaatc tctttcagaa
    24421 ttttttctgg aaagagctta ttgtttttct ggcgcttttt ttgctctttg aagtgtatcg
    24481 ccatgttttt gctaaacaaa aaatgtcagc tgcattgtct ttgattcctg cttttgtttt
    24541 gttggaattt agctggattt tgaatttttt gcctattaat tttttaagcc tcgttgtgat
    24601 ttggattatt ctttttgttt tgatcaatga gttttggctt ttgaattgtg agcggcagtt
    24661 taatttgaaa tattttttac cagagttgct ttttgggttg attttaattg cagttattct
    24721 tttaagcagt tcgtggtgga tgctataacc aattgaggca tggggacgct catttta
//
LOCUS       DAOSMC010000008        19662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_3391_length_19662_cov_16.901260, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000008 DAOSMC010000000
VERSION     DAOSMC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 19662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19662
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_19662_cov_16.901260"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..2349
                     /locus_tag="PL075_01445"
     CDS             511..2349
                     /locus_tag="PL075_01445"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD96892.1"
                     /translation="MKKFWLISLLFAGLMFAGTNLVMAQQTLPAGGDSFESASLIQPG
                     KYQSGSLDEETGLEKYYFIEGVKPGQEIKIKGVFMAGSHSTSNKIILYNEEMEELLNV
                     DDINNSSVVLSASWLPNSDQSSYKYYFKIVNEGSDLWYQTESFTLEISLVDRYDAGSQ
                     TDAGDTIDKALAIAPASNYQSYLAGCCACSPYQIQEGGTDFKDFYKIAVKKGQTLNIE
                     VVPPSEAEMELEIYNTKRQLLESETAENEGAILTTSLNVINDGDIFVAVICGDYCSSK
                     LVAYTLNVTTASAPGEKPVIGEVACGEDSDCPVGLVCSDGQCVSECASDSECGKGLVC
                     QEGVCVYNIFNDFYKQSEGEEGMWKGFPAIVSKTLFSGWRFWLSLVLIIIFYIYFAIC
                     LQTLAKKTNTSNGWLAWIPVANLFLMIQIAQKPLWWFILLLIPIVNIVIGIILWMKIA
                     ERRGKPNWWGILLIVPVVGIIVPGYLAFSGKEKIEITPPYTGTGTQEANKPVVGYQHP
                     CKYCGKLIPPNSAICPFCGKANPLGPYRCPKCHEPIEKEWQVCPNCNQNLRIVCPHCG
                     KITFFGDHCEDCGARLFVKCPHCGQEQPPLTDNCIKCGNPLKVEKR"
     gene            2372..3040
                     /locus_tag="PL075_01450"
     CDS             2372..3040
                     /locus_tag="PL075_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOD96893.1"
                     /translation="MALQPFTDNFTDNSSEAGFQFTFNCALCGEGYQTKFISSKSYKK
                     GKTAKTIGGIFGAAAQMLGKHNIGYGVERATDVLGDKFGEMSPEWRKEHDTAFETSQI
                     EAKAHFHRCPKCTNWVCENCWNEQENLCVSCAPREAVEVVAARAKKKAEDIAGKAAAT
                     QVFTGEIEARQTICPACGKPAGTGKFCVNCGAPLEFVACSKCGAKNPVGTRFCGNCGE
                     KLTN"
     gene            3057..3341
                     /locus_tag="PL075_01455"
     CDS             3057..3341
                     /locus_tag="PL075_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96894.1"
                     /translation="MNQAIFVPQSIFKISGEGVVVTGFLKSGVLKPGMFTEIKKQKIN
                     IINIEAFNKKMEEIKETDLAAKSLAIIVPEGAEKILTGFINQELIFIESK"
     gene            3358..4722
                     /locus_tag="PL075_01460"
     CDS             3358..4722
                     /locus_tag="PL075_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96895.1"
                     /translation="MEEKETSNQQKELQQVEEENINNQQEESPQAEEENKENQFTKTA
                     VGEEKKEPSVLFEVKGDFVLIDETGKESESGQAKFVLDEEKISVASESGKATSLPLRD
                     IGDFFVEDYKIYLLLPNGGKVIIAQLGYQYEDFVRILTNLRHEIIQKELLFKEGIRKS
                     GFEGEYDYQSEGKRHHGRTKVEICDTGLVLKPEQGDLIRIPYSEIREISEKDYKIFIT
                     AQATSLELSLFGEKFNSLSSELKRAMAELSLRTQELLKEFLPGLDPLTIKKAAELLKD
                     GKAINKAELDKISSKIWSELEKELSKIGILESYNYLKSLSKIGEIFVGIKRDLMGDLT
                     GEYVWFLMPISSEKGDNFFVMEAGSTIEGGGKATYLFKMPTGEDIENLVQKFNRCMLA
                     INFRREPIYLKDEDLEKPDYSKYKIAISKIPELKLLRQLFAGRVIHTSPENWKENINK
                     LINP"
     gene            4731..5891
                     /locus_tag="PL075_01465"
     CDS             4731..5891
                     /locus_tag="PL075_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96896.1"
                     /translation="MNLKDFKIDLPTNWREISRFGTENQSSIKIYHISPLSKVDPDIR
                     ENKILPSSNPRVTITRESVPVPRGAPMPIKDYYESMKVAISCGLLSADWTKKIDKLHK
                     DSIKLHCPDDGDFDADIVIAQFQSEEIAKQYFKNMSLMPTQGFDVPMPMGKDTQMPGA
                     PKDLTIYQYLKDFDKYLSKEFLEKYVPKEQLEKMEPEIFEKIKESKPEIKKMQSELKN
                     IQEKKIPEIKKQFLSSGVQYKEGKYLGCDVIYMESKNYSPPPKPKSSSQKSSGIRGGG
                     GLETRFDPLPKIHQPYQKTLIIYQALLIKNFVITGSLLSIINASLPGITPCYSLTKFK
                     KKISTFREGGEVFKDISIVPIVSNYAKEGYLYKEEVEEILKKIIAKLSKNEK"
     gene            6461..7606
                     /locus_tag="PL075_01470"
     CDS             6461..7606
                     /locus_tag="PL075_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96897.1"
                     /translation="MAYKKLSANLKKRKSGSSAVKRKKEPLSKKKSRVKVSPKRKKLA
                     KRELLIKPKDNLIMMPIADHDWEAWQVFNPGVIALEDRIHFIYRAIGVDGISRFGYAS
                     SPDGFLIDERLPRPVYEHPSLNSGLNYYSFGSGGSFGGAEDARLTRVDNEDNIFMTYT
                     ACDEGLRVALTSIKVKDFLKKNWQWEKPVLISPPGEVHKNWVIFPEKINGQYAILHSL
                     RPRVLIAYRDNLNFQKGEYINSFYDGYNEETGWEIRIRGAGAPPIKTKYGWLVFYHAN
                     DKSDPNKYKVGAILLDLMNPETTLMRSSKPILEPDEPFQSNGFKPGIVYVSGAVVKDG
                     TLFIYYGVADNYICVAWANFDEFLNDLVQQTKPKLKQKVILNKKDAR"
     gene            7625..8596
                     /locus_tag="PL075_01475"
     CDS             7625..8596
                     /locus_tag="PL075_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOD96898.1"
                     /translation="MNFLESIEKFPQQFLFQPQIKNIERLKKADYFIVLGMGGSALAA
                     DLLKARFPKKPLFIHKNYGLPSLAEQILKKSLVIACSYSGNTEEVLDGYNEAQKKKMM
                     VAVISSGGKLLNKAKKNKSPYIQIPNFNIQPRLSLGLMSKALAAFMQDKILFQELSSL
                     ARILKPQSFQKQGQKIARTLYKHIPIIYASTANEALAYHWKISFNENTKIPAFYNVFP
                     ELNHNELSCFDLHSSTKILSEKFSIVLLEDKKDEPRIQKRVAVLKKILRTNNFNIQEI
                     KLLNHSESEKIFSSVLLANWSSYYLAKKYFIEPESVPLVEKFKKLIK"
     gene            8593..10515
                     /locus_tag="PL075_01480"
     CDS             8593..10515
                     /locus_tag="PL075_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96899.1"
                     /translation="MIIKRSPQNPILKPLREHGWEAEATFNPCAVVKGKDIFLLYRAI
                     SMPHYHSWARSNIVTSDIGIAQSHDGHYFFNRKRFIVPEEDWERFGCEDPRVTKLNDK
                     YYIFYTALSKYPFRAEGIKVGLAISKDLKNIQEKHLVTPFNAKAMALFPEKINGQITA
                     ILTVHTDQPPAKICLASFNKESDIWSEKFWNEWYKNFEKYALPLQRNKEDHIEVGAPP
                     LKTKYGWLIFYSYIRNYFSPDRLFGIEAILLDLKNPSKILARTEYPILTAEEYYEKIG
                     FVPNVVFPSGAFIKDNWIYLYYGGADTVGCLALIHLPTFFNEMLMEDSKKPKLERFPN
                     NPILLPRQENFWEKKAVFNPGAIYLNNKVHLIYRAISEDNVSTFGYAQSSDGYHIDWR
                     SDQPIYFPRESFEKRDNPNVSCGCEDPRLTKIGNKIYMGYTAFDGRLPRIALTSIGVK
                     DFLNQQWQNWARPVLISPADLNDKDMVIFPEKFQGQYLIVHRVGYDIDYSFSKDLNFG
                     EGKWLEENRWIYCRPGWWDSKKIGVAAPPLKTKNGWILFYHGISENNVYRVGAILLDL
                     KNPIKILGRTTKPLLEPKMPYEKEGVVNNVVFPCGAVEIEGKVFIYYGGADRVVCGAT
                     INLAKLLQIFKIDKEY"
     gene            10581..11411
                     /locus_tag="PL075_01485"
     CDS             10581..11411
                     /locus_tag="PL075_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOD96900.1"
                     /translation="MNKAITIFLIIVVIGLGIAVIFKLTQNQTPQKVNLQVTEYQDPV
                     ALKKVIDELEARNLKPATIFVGGELAKNNCQLIKDLDQKGYEIAAFGYALDENGEFIQ
                     LADLTRKKQEEVIKNTKEDIENCLGHKINGFRSQRFSQNKDTIEIVKNLGFSWNGSFV
                     VNWHPEASFIPYYSNDYGFYVVSIEGVKNTGYVLCDTATASFGKTAKEWRETIQEYFN
                     IHQKEETPFITEFHPYFLTDNSDWWDEFIKLLDWFKKQNANYLTTQQLIDSSCLICGE
                     "
     gene            11469..12404
                     /locus_tag="PL075_01490"
     CDS             11469..12404
                     /locus_tag="PL075_01490"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HOD96901.1"
                     /translation="MKKNYKIIICVVIIVLIGVGIWIYLNQPQLSSVNKTMVNSDVGE
                     GLVLINHINYLCKDDKIVEAYFYQGPEVSSLPGEPPVATGKAKIILSDGRNLILNQSI
                     AADGVRYTNEDESFIFWTKGPTALVLENNEQKNYQDCMALSKESKDLPNTYLDAQKGF
                     SLRYPADYSVDTDYKYQGLGPDKQIRGVKFTIPESMARGTNLSSFDTGVSIEIIPTVE
                     NCNAGMFFGGDVKSQLKTDGDREHSFASLSEGAAGNFYEEQVWAFNGMKYCFGVRYLI
                     HSTNIENYPEGSVSQFDKNALLEQFDKIRRSLIVL"
     gene            12592..13002
                     /locus_tag="PL075_01495"
     CDS             12592..13002
                     /locus_tag="PL075_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOD96902.1"
                     /translation="MKIIISTKGNNDIIDITEEIVRAVKESKAKEGFCLVFCPGSTVG
                     ITNLEAEPNLIEDFKEFLEKLAPSDKAYHHDETWGEKNGFSHLRSALIKPFFVAPIEN
                     GDLILGNWQQIVLVDFDSRPRQREIIIKIYNFNY"
     gene            13067..13351
                     /locus_tag="PL075_01500"
     CDS             13067..13351
                     /locus_tag="PL075_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOD96903.1"
                     /translation="MARFVIKKSGEKEPFDPIKIGDSIRAAAAEANLTPERTESIVNR
                     VAAVIIEMAAQKEEIATSEIRARILKELEILEPSVAEVWKKFEAEHNKTA"
     gene            13421..13732
                     /locus_tag="PL075_01505"
     CDS             13421..13732
                     /locus_tag="PL075_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HOD96904.1"
                     /translation="MFRKKSKKYKKQKNWSSEVTQNSFALELDSGVFTWKNPRRIALS
                     LKRSAEKSFNRKGTPFQSAMSMLNFYINRAGKKLKLDQKQILESAKQELRILFHKEKL
                     K"
     gene            13729..14307
                     /locus_tag="PL075_01510"
     CDS             13729..14307
                     /locus_tag="PL075_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOD96905.1"
                     /translation="MKKEKFLIIVGVIIAIVIFTIFIFREKNIFQNQKQVKPSILNNL
                     NTMKVSSPAFENNKSISSKYTCEGENINPPLLIENIPSETKSLVLIVDDPDAPRGTFI
                     HWVVFNIPPQTRLIEENSIPEGALLGKNDANDSQYYGPCPPSGKHRYFFHLYALDIVL
                     DLKNGADLSEIKSAMRNHILDETELIGTYQRT"
     gene            14398..15189
                     /locus_tag="PL075_01515"
     CDS             14398..15189
                     /locus_tag="PL075_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOD96906.1"
                     /translation="MAEKRIREKVSNAVDYEIFKDSFFQAEKGITRKAIALPVAIILH
                     VVFIAAIIIIPLLSPGQDLPKVEIYSAFLAPPPPPPPPPPPPAKKKTSSTRTIKAVQA
                     TTSVEPGKLVAPVEIPDEIQQEVISDFGIEGGVEGGVEGGVIGGVLGGVVGGVIGGVI
                     SQQMESEEPVRAIGEIKQPKLIHRVEPVYPEIARQARVEGIVILECTTDIYGRVQSVN
                     VLRSVPLLDQAAIDAVKQWVYEPMVINGRPRGVVFTVTVNFTMKK"
     gene            15285..15866
                     /locus_tag="PL075_01520"
     CDS             15285..15866
                     /locus_tag="PL075_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOD96907.1"
                     /translation="MIKDEQLVKNYLNGDEEAIAILINRYLKEVYLYFLKRLNNSIEA
                     EDLTQETFVKMWRNLKKFDQKRNFRTWLFAIAKNLLIDFFRKHQTTNGLSKEVTFSQF
                     INQEDEENVILDKIIDWQPSAIEQIEEKETREKLRSIINGLPLAQKKLLFSYLESDVS
                     FEKLAQEINIPTNTLKARYYRLIQQIKKLFLQQ"
     gene            15941..16342
                     /locus_tag="PL075_01525"
     CDS             15941..16342
                     /locus_tag="PL075_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96908.1"
                     /translation="MNKVIAAKHTNEDVLLKKILWKIREEKNKRIRKEVLLSFCGLMV
                     SLALLPLSFQLSYSNFIETGFIYYLGTIIMHWELWSSFLNEFIFILLESLPTVGLLLV
                     LTNLFMLAFSLKYLLKNSSHFSFKFLFSRLI"
     gene            16348..16851
                     /locus_tag="PL075_01530"
     CDS             16348..16851
                     /locus_tag="PL075_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96909.1"
                     /translation="MKLEDISSSKTFKIIIGIIGGLIAILLIFKIGVVVGTKKALFAC
                     RWAEHYAPNFVQQRNGIMDKWMRDFNEGIAMKSHGAVGTIIKIDNLDTNTAFLIVKGE
                     KEVEKAIVVSPETIIEKWEERILPADLRMGDYVVVIGDANDEGQIEAKLIRVIPHPFP
                     PEHLGGL"
     gene            16895..18568
                     /locus_tag="PL075_01535"
     CDS             16895..18568
                     /locus_tag="PL075_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOD96910.1"
                     /translation="MNKIINFLKNHRIIAVLIILIIVSGGYFLLKPLFFKNTAAQYLT
                     AKVERGNLTSSITVSGQTIAENSVNLSPKASGEVVYVNLANGKQVEAGETILKIDDED
                     VRSELKDAQIALNNAKEELAKLEGLSTEEGSIESLKIKRQKDLTLAYQNAVDKLPALF
                     TSTTPAIITNLYNILFDNNLSPDDDNVDYYGKTLMLYNPAALQYSDATKKAYDLAKFS
                     YEEAANNYKSKNITYYSDSNEIDEAINLTHQALKNINNAVRAALNLVHLYKDFMIEAK
                     LQYPSLVDEQDLMLTNYFATTTNLFSQIDTIKNNIQIAKENLINVDFDLSDQKRLVEK
                     AERNLKLIQDKLADYTVLAPFSGILGNVATIYVGDNVSPSTTLGVLVSQGYVVEVSLA
                     ESDAAQVKIGQKANVSFDVLDDVAATGKVIEIDTVGVVVQGVVSYKAKILLDVIDERV
                     KPGMSTTVEIITDAKENTLFIPRTALKNLNGKYFVRILKDDGTIEQREIKTGLLTDSK
                     VEIIQGLNEGEKIIIADLNSSSGVNLLRTNFSNQRNNQTQIPRGIPGAF"
     gene            18573..19295
                     /locus_tag="PL075_01540"
     CDS             18573..19295
                     /locus_tag="PL075_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOD96911.1"
                     /translation="MQEKNIIEVKNLSKIYDGVVETIALRNVNMSIREGEFVSIIGPS
                     GSGKSTLMHILGCLDKSTSGEYIFEEKNISHFSEDELADIRKTKIGFVFQQFNLLPRA
                     TVLRNVALPLVYANISKKEREVRAAKVLEETAFPKEFWNHLPNQLSGGMMQRVAIARA
                     LVNDPILILADEPTGNLDSKTGEKILATFKELHHRGRTIILVTHDRYIAEQAKRIISL
                     KDGEIIEDKKIEESEEFKIMRE"
     gene            19298..>19662
                     /locus_tag="PL075_01545"
     CDS             19298..>19662
                     /locus_tag="PL075_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOD96912.1"
                     /translation="MLISDLFSETTSAILSNKLRSSLTILGIVIGIGSVIGMISIGSG
                     SKTNIESRLQSMGTNMIYVMPGMQTNFSPVSQGRGTAQSLTIDDVKAIEEIPYVSDVS
                     PIATRRFQVISKSSNTNTQ"
BASE COUNT         6809 a         2844 c         3784 g         6225 t
ORIGIN      
        1 cccagaagaa aatggaagaa ttggcagaag aatttaagaa aaaaggagtt attcgcagtt
       61 ggaagttaaa agatgtggtt tttgatttag atatagaaaa taaacaaatt aaaggaataa
      121 cgcctactga ttgggagagg acgaagattg aagaataagc ttttatagaa tacattttaa
      181 cacactgtct accgcatgga attttgtacc gcgcagggcg cacgaccatg aatcttgcgt
      241 gtggtttgcg gccataaatc atcatatata cttttttaat cccatcaaat tttgaatttt
      301 ccctaaaaac cctcatttat tgagggtttt taaattaaag ttatccactt aattacttgc
      361 ttttgtattt tatatttgtt tttgttataa ttaaaatgtt tagatttcat tgtgaaatta
      421 gataattaat aatataatat ttataatgat taaaaattaa ttaaattaag gaaaaaggtc
      481 aaaaataaaa aagtaaataa aataaaaaac atgaaaaaat tttggctcat tagtctttta
      541 ttcgccggtt taatgtttgc cggtactaat ttggtaatgg ctcagcaaac attgccagcc
      601 ggtggagata gttttgaatc agcaagtttg attcagccgg gtaaatatca aagcggttct
      661 ttagatgaag agacggggtt ggaaaaatat tattttatag aaggagtaaa gcccggtcag
      721 gaaattaaaa ttaaaggagt ttttatggcg ggtagtcata gtacgtccaa taaaataatt
      781 ctttataatg aagagatgga agaattatta aatgtcgacg atataaataa ttcttctgta
      841 gtactttctg cttcttggct gcctaactct gaccagtcaa gttataaata ctactttaaa
      901 attgttaatg agggtagtga tttgtggtat cagacagaat cttttacttt agaaatttct
      961 ttggttgatc gttatgatgc tggtagtcaa accgatgctg gcgataccat tgataaagct
     1021 ttggccattg ctccagcaag taattatcaa tcatatctgg ctggctgttg cgcttgttcg
     1081 ccatatcaga tccaagaggg aggaacggat tttaaagatt tttacaaaat tgcagttaaa
     1141 aaaggacaga ccttaaacat agaagtagtg cctccttcag aagccgaaat ggagttagag
     1201 atttataata caaaaagaca attattggaa tctgaaacag ctgaaaatga gggagcaatt
     1261 cttactactt ctttgaatgt aattaatgac ggtgatattt tcgtggctgt catttgcggg
     1321 gactattgta gttcaaaact tgttgcttac actttgaatg ttaccaccgc aagtgcgccg
     1381 ggagaaaaac cggtaattgg tgaagttgcg tgcggtgaag attccgattg cccagtgggc
     1441 cttgtttgtt cagatggtca atgtgtttct gaatgtgcga gtgattctga gtgtggaaaa
     1501 ggattagtat gccaagaagg agtgtgtgtt tataatattt ttaatgattt ttataaacag
     1561 agtgaaggag aagaaggaat gtggaaaggg tttcccgcga tagtttctaa aacattattt
     1621 agcggttggc gtttttggct ttctctggta ttaataatca ttttttacat ttattttgca
     1681 atttgcttac aaacattagc aaagaaaact aatacttcca atggttggct tgcttggatt
     1741 ccagtggcta atctttttct aatgattcaa attgctcaaa aacctttgtg gtggtttatt
     1801 ttactgttaa ttcctatagt taatatcgtt attggcatta ttctctggat gaaaattgca
     1861 gaaagaagag gaaagccgaa ttggtgggga attttactta ttgtgccggt agttggtatt
     1921 attgttcccg gttatttagc cttttctgga aaagaaaaaa tagaaattac tccaccgtat
     1981 actggcacgg gaacacaaga agccaataaa ccagtggttg gttatcaaca tccttgtaaa
     2041 tattgcggaa aattaattcc gccaaattct gctatttgtc ctttctgcgg aaaagctaat
     2101 ccacttggac cttatcgttg tcctaaatgc cacgaaccaa ttgaaaaaga atggcaggtc
     2161 tgtccaaatt gtaaccaaaa tctgagaatt gtttgtcctc attgcggaaa aataaccttt
     2221 ttcggcgacc attgcgagga ttgtggagca cgtctttttg ttaaatgtcc tcattgtgga
     2281 caagaacaac caccgctaac agataattgt attaaatgcg gaaaccctct taaagtcgaa
     2341 aagaggtaaa tttagaaatc tttaaaaata tatggcactt caaccattta cagataattt
     2401 taccgataat tcaagcgaag ctggctttca atttaccttc aattgcgctc tttgtggcga
     2461 aggttatcaa acaaaattta tttcctcaaa gtcttataaa aagggaaaaa cagcaaagac
     2521 tattggcggt attttcggag cagcggctca aatgttagga aaacataata tcggttatgg
     2581 agtagaaaga gccaccgatg ttcttggcga taaatttggc gaaatgtcac cagaatggag
     2641 aaaagagcat gatacggctt ttgaaacttc ccagattgag gccaaggcgc attttcatcg
     2701 ctgtcccaaa tgtaccaatt gggtttgcga aaattgttgg aatgagcaag aaaacctttg
     2761 tgtttcttgc gcgccaagag aagccgtaga ggtagttgcc gccagagcta agaaaaaagc
     2821 cgaagatatt gccggcaaag cagcggcaac ccaagttttt accggtgaaa tagaggcaag
     2881 gcaaactatc tgtcctgcct gtggcaagcc ggctggaacc ggtaaattct gcgttaattg
     2941 cggtgctccg ttagaatttg tagcatgttc taagtgtgga gcaaaaaatc cggttggtac
     3001 cagattttgc ggcaattgcg gagaaaaatt aacaaattaa tatatttaat aaatatatga
     3061 atcaagcaat ttttgtacca caatctatat ttaaaattag tggagagggt gtagttgtta
     3121 ctggtttttt gaaaagcggt gttttaaagc cgggaatgtt tacagaaata aaaaaacaaa
     3181 aaataaatat tattaatata gaagcattta ataaaaaaat ggaagaaatt aaagaaacag
     3241 atttagccgc taaatcactg gcaataattg tgcccgaggg cgcggaaaaa attttaacag
     3301 gttttataaa tcaagaatta atttttatag agtcaaaata aattattaaa ttattatatg
     3361 gaagaaaaag aaacaagcaa tcaacaaaaa gaattgcaac aagtcgaaga agaaaatata
     3421 aacaatcaac aagaagaatc accacaagct gaagaagaaa ataaagaaaa ccaatttact
     3481 aaaactgctg taggtgaaga aaaaaaagag ccgtctgtcc tttttgaggt aaaaggggat
     3541 tttgttttga ttgatgaaac gggaaaagag agtgaatccg gccaagcaaa atttgttctg
     3601 gatgaagaaa aaatttccgt tgcttcagaa agtggcaagg caacttctct tcccttgaga
     3661 gacattggtg atttttttgt tgaagattac aaaatctact tgctgctacc caatggtggg
     3721 aaagtaataa ttgctcaatt aggttatcaa tatgaagatt ttgttagaat acttactaat
     3781 cttcgccatg aaattattca aaaagaactt ttatttaaag aaggcattag aaagtctggt
     3841 tttgaaggag aatatgatta tcaaagcgaa gggaaaagac atcatggaag gacaaaggtt
     3901 gaaatttgcg atacgggttt ggtattaaaa cccgagcaag gggatttaat cagaattcct
     3961 tatagtgaaa ttagagaaat ttccgaaaag gattataaaa tttttatcac tgctcaagca
     4021 acttcactag aactatctct ttttggagaa aagtttaata gtttaagcag cgaattgaaa
     4081 cgggccatgg cggaattgtc tttgagaact caagaacttt tgaaagaatt tttaccgggg
     4141 ctagaccctt taacgattaa aaaagccgcc gaacttttaa aagatggcaa agcgattaac
     4201 aaagccgagc ttgataaaat ttcgtcaaaa atttggtccg agttggaaaa agaattaagc
     4261 aaaataggta ttttagaatc ttacaattat ctaaaatctt tatccaaaat cggagaaatt
     4321 tttgttggca ttaaaagaga tttaatgggt gatttaaccg gagaatatgt ttggttttta
     4381 atgccaattt cttctgaaaa gggcgataat ttttttgtta tggaagcggg ctctactatt
     4441 gaaggcggcg gaaaggcaac ttatttattt aaaatgccta cgggtgaaga tatagaaaat
     4501 ttagttcaaa agtttaatcg ttgcatgtta gctataaatt ttcgtcggga accaatttat
     4561 ttgaaagatg aagatttgga aaaacctgat tattcaaaat ataaaatagc catcagtaaa
     4621 attccggaat taaaacttct tcgtcaactt ttcgctggcc gagttattca tacatctccg
     4681 gaaaattgga aagaaaatat aaacaaactc attaatccgt aattactttt atgaacttaa
     4741 aagattttaa aattgatcta ccaacaaact ggcgggaaat ttctcgtttt ggcactgaaa
     4801 atcaatcttc aataaaaatt tatcacatca gtccattatc aaaagttgat ccagatatac
     4861 gcgaaaataa aattttaccc tcttcaaatc ccagggtaac tattactagg gaaagtgtcc
     4921 cagtgcctag gggggcgccc atgcccataa aagattatta cgaaagtatg aaagtagcga
     4981 tttcatgcgg tttgttgtct gctgattgga caaaaaagat tgataagttg cataaagatt
     5041 caattaaact tcattgtcct gatgatggtg attttgacgc tgatattgtc attgctcaat
     5101 ttcagtccga agaaattgct aaacaatact ttaaaaatat gagtttaatg cccactcaag
     5161 gatttgatgt tcctatgccc atggggaaag acacccaaat gcccggagcg ccaaaagatt
     5221 taactatcta tcaatatcta aaggattttg acaaatacct atccaaggaa tttttggaaa
     5281 aatatgtacc caaagagcaa ttagaaaaaa tggagccgga gatttttgaa aaaattaaag
     5341 aatcaaaacc ggagataaaa aaaatgcaat ccgaattaaa aaatatccaa gagaaaaaaa
     5401 tacctgaaat aaaaaaacaa tttttaagtt ccggagttca atataaagaa ggaaaatact
     5461 taggttgcga tgttatttat atggaaagca aaaattattc gccaccgcca aaaccaaaat
     5521 catcatctca aaaatcctcc ggaattcggg gaggaggtgg tttggaaacc agatttgatc
     5581 ccttaccaaa aatccatcaa ccataccaaa aaactcttat tatttatcag gctcttttga
     5641 ttaaaaattt tgttatcacc ggtagtttgt tgtcaataat taatgcttca ctgccgggca
     5701 taacaccttg ttattcatta acaaagttta agaaaaaaat ttcaactttc agagagggag
     5761 gagaagtatt caaagatatt tctatagtgc caatagtaag caattacgct aaagagggtt
     5821 atttatacaa agaagaggta gaagaaattc tcaaaaaaat aatcgcaaaa ctatcaaaga
     5881 atgaaaaata agtatttaag tatactatta ctctaaaaaa ggatttattg tcaaagaatt
     5941 actttaacta aaacaaaaaa tctactcgtt ctgagcaatt ttgattttaa aaaatttctg
     6001 atataatttt attggaatag cgagaattgg tattcatcgg ctttgctgat ggcagtgttg
     6061 cgcaataagt ccgccatccg tctttgtgcg gtggatttac tcgaaagggt agaaggtgtg
     6121 gtgaaaagca acattgattt acggatatac caagagccgc attcgcggga gcgaacaaag
     6181 aataaaacaa acaatgaaat cttgtttttc tttcagcagc ttgctttcca ataaaaacct
     6241 cccatttctg ggagtttttg ttggaataaa taatgagtag cggggtaagg caagtttatg
     6301 catagtaggt attagcatat ttttcaaatt aggggaggtg tttttttgtt tattttatta
     6361 tatggtataa ttaaataaat ttgatataat aaaaagagag catttattca ttagtagcca
     6421 ttggccaatt ttaataagag ttaagaataa tttttaatca atggcctata aaaaattaag
     6481 cgctaattta aaaaagagaa agtcaggaag ttcagcggtg aaacgaaaaa aagaacctct
     6541 ttctaaaaag aaaagtagag ttaaggtttc gccgaaaaga aagaaattag caaaaagaga
     6601 gctattgatt aagcctaaag ataatctcat tatgatgccg attgctgatc acgattggga
     6661 ggcgtggcaa gtttttaatc ctggtgttat tgctttagaa gacagaatcc attttattta
     6721 tcgagcaatt ggtgttgatg ggatttcgcg ttttggctat gcttcttcgc cagatggttt
     6781 tttaattgat gagaggttac cacgtcctgt ttatgagcat ccttcattaa attctggttt
     6841 aaattattat tcgtttggtt cgggcggaag ttttggcgga gcagaagatg cacggcttac
     6901 tcgagttgat aacgaagata atatttttat gacctatact gcttgcgatg aagggttgag
     6961 agtggctctt acttcaataa aagtcaaaga ttttttgaaa aaaaattggc aatgggaaaa
     7021 gccggttcta atttcaccac cgggtgaagt tcataaaaat tgggttattt ttccagaaaa
     7081 aattaatggc caatacgcta ttttacatag tttaagacca agagttttga ttgcttatcg
     7141 agataacttg aattttcaaa aaggggagta cattaatagt ttttatgatg gttacaacga
     7201 agaaacaggg tgggaaatta gaattagagg cgctggagcg ccacctatta aaacaaaata
     7261 tggatggtta gtattttatc atgctaatga caaatcagat cctaataaat ataaagtggg
     7321 agcaatactt ttagatttaa tgaatcctga aacaactctg atgcggtctt caaagcctat
     7381 tcttgagcca gatgaacctt ttcagtctaa tggttttaaa cccggcattg tttatgtttc
     7441 aggcgcggta gttaaagatg gcaccctttt tatttattat ggcgtagcgg ataattatat
     7501 ttgtgttgcg tgggctaatt ttgatgaatt tttaaatgat ctcgttcaac agacaaagcc
     7561 aaaattaaag caaaaagtga ttttgaacaa aaaagatgct cggtaatttt aataaaattg
     7621 taagatgaac tttttagaat ccatagaaaa atttcctcag caattccttt ttcaacctca
     7681 aattaaaaat attgagcgtt taaagaaggc ggattatttt attgttttgg gaatgggcgg
     7741 ttctgcattg gcagctgatc ttttaaaagc tcgttttccc aaaaagccgc tttttattca
     7801 taaaaattat ggtctaccat ctttggcaga gcagattttg aaaaaaagtc ttgtgattgc
     7861 ctgttcttat tccggcaata ccgaggaagt gcttgatgga tataatgaag ctcaaaaaaa
     7921 gaaaatgatg gtagctgtta tcagtagcgg tggcaaactt ttaaataaag ccaaaaaaaa
     7981 caaatcgccg tatattcaaa ttcctaattt taatattcaa ccacgtcttt ccctcggatt
     8041 aatgagtaag gctttagctg cttttatgca agacaaaata ctttttcaag aactttcttc
     8101 tttggctcgg attttgaaac ctcagtcgtt tcagaagcag gggcaaaaaa tagcccgtac
     8161 tctttataaa cacataccaa ttatttatgc ctccacagcc aatgaggccc tggcttatca
     8221 ttggaaaatt agttttaatg aaaatactaa aatccccgct ttttataatg tttttcccga
     8281 attaaatcac aatgaattaa gttgttttga tttgcattcg tcaaccaaaa ttttatcaga
     8341 aaaatttagc attgttttat tggaggataa aaaagatgaa ccacgtattc aaaaaagagt
     8401 ggctgtttta aagaaaattt tgcgaacaaa taattttaat attcaagaaa ttaaattatt
     8461 aaatcattcg gagagtgaaa aaatattttc ttctgtgtta ctggctaatt ggagttctta
     8521 ctatttagct aaaaaatatt ttattgagcc agaaagcgtg cctttagtcg aaaaatttaa
     8581 aaagttgatt aaatgataat taaacgttcg cctcaaaatc ctattttgaa gccattacga
     8641 gaacatggtt gggaagccga agcgactttt aatccatgcg ccgttgttaa aggcaaagat
     8701 atctttcttt tatatcgggc tatttcgatg ccgcattatc attcgtgggc ccgatctaat
     8761 attgttacgt ctgatatagg aattgctcaa agtcatgatg gccattattt ttttaatcga
     8821 aaaagattta ttgtaccaga agaagattgg gagcgatttg gatgtgaaga tcctcgagtg
     8881 acaaaattaa atgataaata ttatattttt tatactgctc tttctaaata tccctttcgg
     8941 gcagaaggta taaaagttgg attagccatc agtaaggatt taaaaaatat tcaagaaaaa
     9001 catttagtaa caccttttaa cgctaaagcc atggctttgt ttcccgaaaa aattaatggt
     9061 caaatcacgg caattttaac tgttcatacc gatcaaccac cagcaaaaat ttgcttagcc
     9121 tcttttaata aagaaagcga tatctggtca gaaaaatttt ggaatgagtg gtacaaaaat
     9181 tttgaaaaat atgccctgcc acttcaaaga aataaagaag atcatattga agtaggagcc
     9241 ccgcctctta aaacaaaata tggctggtta attttttata gttatattcg taattatttt
     9301 tctccagatc gattatttgg cattgaggca attttattag atttaaaaaa tccgtctaaa
     9361 attttagcga gaactgaata tcccatttta acagctgaag aatattatga aaaaatcggc
     9421 ttcgtgccta acgttgtttt tcccagcggt gcttttataa aggataattg gatctatctt
     9481 tattatggcg gtgccgatac agttggttgt ttggcattaa ttcacctccc tacatttttc
     9541 aacgaaatgc taatggaaga tagtaaaaaa ccaaaattag aacgttttcc caacaatccc
     9601 attctgcttc ctcgtcaaga aaatttctgg gaaaagaagg cagtttttaa cccgggcgca
     9661 atttatttga ataataaagt ccatttaatt tatcgagcaa tttctgaaga taatgtttct
     9721 acttttggtt atgctcaaag ttcagacgga tatcatattg attggcgatc tgatcaaccc
     9781 atatattttc ctcgcgagag ttttgaaaaa agggataatc ctaatgtttc ttgcggatgc
     9841 gaagatccgc gattaacaaa aataggaaat aagatttata tgggctacac ggcttttgat
     9901 ggtcgtcttc cgagaattgc acttacttcc attggtgtta aagatttttt aaatcaacaa
     9961 tggcagaatt gggcaaggcc agttttgatt tcaccggctg atttgaatga taaagatatg
    10021 gtaatttttc cagagaaatt ccaaggtcaa tatctgatag ttcatcgtgt aggttatgat
    10081 atagattact ccttttctaa agatctaaat tttggagaag gaaagtggtt agaagaaaat
    10141 agatggatct attgtcgtcc aggttggtgg gatagtaaaa aaataggagt agctgcgcca
    10201 ccgctgaaaa ctaaaaatgg ctggatttta ttttaccatg gtatctccga aaataatgtt
    10261 tatcgagtag gggctattct gttagatctc aaaaatccta ttaaaatttt aggtcgaacg
    10321 acaaaacccc ttttagaacc aaaaatgcct tatgaaaaag aaggagtggt taataatgtt
    10381 gtctttcctt gtggagcagt agaaattgag ggtaaggttt ttatctatta tggcggagcg
    10441 gatcgagtag tttgtggggc tactataaat ttggcaaaac tgcttcaaat tttcaaaata
    10501 gacaaggagt attaattgaa caaacataga aattattgat catcttgttt tcgtcacctt
    10561 ttttgcttat aataaaaaat atgaataaag caatcactat ttttttaata attgttgtta
    10621 tagggttggg aatcgctgtc atttttaaac taacccaaaa tcaaacgcca cagaaggtta
    10681 atcttcaagt aaccgaatat caagatccag tagcccttaa aaaagtaatt gatgaattag
    10741 aagctagaaa tttaaagccg gctactattt ttgtcggcgg tgagttagca aaaaataatt
    10801 gccaacttat taaagattta gatcaaaaag gttatgaaat tgcagctttt ggctatgcgt
    10861 tggatgaaaa tggcgaattt attcaattag ctgacttaac aagaaaaaaa caagaagaag
    10921 ttattaaaaa tactaaagaa gatattgaaa attgtttggg tcataaaata aatggtttta
    10981 ggtcgcagag atttagccaa aataaagaca cgattgaaat tgtgaaaaat ttaggattta
    11041 gttggaatgg cagttttgtt gttaattggc atccagaagc ttcttttatt ccatattatt
    11101 ctaatgacta tggtttttat gtggtatcta ttgaaggagt aaaaaatacc ggctatgttt
    11161 tatgcgatac agccacagct tcttttggaa aaacagctaa agaatggcgc gaaactattc
    11221 aagaatattt taatattcat caaaaagaag agactccctt tatcaccgaa tttcatccat
    11281 attttttaac tgataattct gattggtggg atgaatttat taaacttttg gattggttta
    11341 aaaagcaaaa tgccaattat ttaactactc aacaactcat tgattcttct tgtcttatct
    11401 gtggtgaata atttattgtt gtattaaaat caattaagaa aaggccataa ttaaataaaa
    11461 cacatcttat gaaaaaaaat tataaaatta ttatttgcgt tgtaataatt gttttaattg
    11521 gtgtgggaat ttggatttat ttaaatcaac cccaattaag ttcggtcaat aaaacaatgg
    11581 ttaattctga tgttggagaa ggcttggttt taataaatca cattaattat ctttgtaaag
    11641 acgataaaat agtagaggcc tatttttatc aaggaccaga agtttcgtct ttgcccggtg
    11701 agccgccagt agcaaccgga aaagcaaaaa ttattttaag cgatggccgc aatttaattt
    11761 taaatcagag tattgcagct gatggcgttc gctatactaa tgaagatgaa tcgtttattt
    11821 tttggacaaa agggccaaca gctttagttt tggaaaataa tgaacaaaaa aattatcaag
    11881 attgcatggc tttatcaaaa gaatcaaagg atttacccaa cacttattta gatgctcaaa
    11941 aaggattttc tcttcggtac ccagctgatt attcagtaga tacagattat aaatatcagg
    12001 gattaggccc agataaacaa atacgaggtg ttaaatttac tattcccgaa agtatggcca
    12061 gaggcactaa tttatcaagc tttgataccg gtgtatcaat tgaaataatt ccaactgttg
    12121 aaaattgcaa tgccggaatg ttttttggtg gcgatgtaaa aagccaatta aaaacagatg
    12181 gtgacagaga acattctttt gcttctttaa gtgagggagc cgctggtaat ttttatgaag
    12241 agcaagtctg ggcatttaac ggaatgaaat attgttttgg agtgcggtat ttaatacatt
    12301 ctacaaatat agaaaattac ccagaaggaa gtgtttcgca atttgataaa aatgctttat
    12361 tagaacaatt tgataaaatt cgccgctcac taatagtttt ataagatata attaattttt
    12421 ttcgaattac gtttttaaag atttaattgg tttacttttt tgatttatta atattattta
    12481 ttaaagaatt ctccaaactt ttattgctat tttttctcat ttttgtaggc aaaaggtttt
    12541 gaaagatagc gaattataaa ttagaaaaat ctttaattta aatatttatt gatgaaaata
    12601 attatttcaa caaaaggcaa taatgatata attgatatta ctgaagaaat tgttcgagca
    12661 gtaaaggaaa gtaaagcaaa ggagggtttt tgtttggttt tttgtccggg gtcaacggta
    12721 ggaattacta accttgaagc tgaacctaat cttattgagg acttcaaaga atttttagaa
    12781 aaattagcgc cttcggataa agcttatcat catgatgaaa cttggggaga gaaaaatgga
    12841 ttttctcatt taagatcggc actgattaag ccgttttttg tggcaccaat tgaaaatgga
    12901 gatttaatac tcggcaattg gcagcaaatt gttctagttg attttgatag tcggccgcgg
    12961 caaagagaga taataattaa aatttataat tttaattatt aggagtattg cgatttaact
    13021 atttaaagat ttataataaa attaaaggtc ggcattaaca aaaattatgg ctagatttgt
    13081 tattaaaaaa tcaggagaaa aagagccgtt tgatccgata aaaataggtg attctattcg
    13141 ggcagctgca gccgaggcta atttaacccc cgaacgaaca gaaagcatag taaatcgtgt
    13201 cgcggcagtt attattgaga tggcggcgca aaaagaggaa attgccactt cagaaattag
    13261 agcccgcatt ctaaaagagt tggaaattct tgagccttcg gttgctgaag tgtggaagaa
    13321 atttgaagca gagcataata aaaccgccta aacgaaaaga gactttttgc ttggatctct
    13381 ttaaaagaga aaatttttgt tattaaaaat tattgagtga atgtttcgga aaaagtctaa
    13441 aaaatataaa aagcaaaaaa attggtcatc cgaagtaact caaaatagtt ttgctttaga
    13501 attagattca ggcgttttta cttggaaaaa tcctaggagg atagcccttt cacttaaaag
    13561 atcagccgag aaaagcttta atcgtaaagg gacgcctttt caatcggcaa tgtcaatgtt
    13621 aaatttttat attaatcgag ccggaaaaaa attaaagttg gatcaaaagc aaattttaga
    13681 atcagcaaaa caagaattga gaatattatt tcataaagaa aaacttaaat gaaaaaagaa
    13741 aaatttctca ttattgttgg cgttattatt gcaattgtaa tttttactat ttttattttt
    13801 cgagaaaaaa atatttttca aaatcaaaaa caagtcaaac catctatttt aaataattta
    13861 aatactatga aagttagcag ccctgctttc gaaaataata aatctatttc ttcaaaatat
    13921 acttgcgaag gagaaaatat taatccgcct ctacttattg agaatattcc ttctgagacc
    13981 aagagtcttg tattaattgt agatgatcct gatgcgccgc gaggaacttt tattcattgg
    14041 gtggtattta atattcctcc ccaaactaga ttaattgaag agaacagtat tcccgagggc
    14101 gcgcttttag gtaaaaacga tgctaatgat tctcaatatt atggtccttg tccaccatcg
    14161 ggaaagcatc gatatttctt ccatttatat gcattggata tagtattgga tttaaagaat
    14221 ggagcagatc tttccgaaat taaatcagcg atgcgcaatc atattttaga tgagacagaa
    14281 ctcattggca catatcaaag gacataaaaa gtaagaatat tagtactatt gacaaaaaga
    14341 acccatatct tatattaaag atagcaattt aattttaaaa gaaaggaggt gagtaagatg
    14401 gctgaaaaaa gaattagaga aaaagtttcc aatgcagttg attatgagat ttttaaagat
    14461 agtttttttc aagcagaaaa aggaattaca cgaaaagcca tagctttacc cgtagctatt
    14521 attcttcatg tggtatttat tgctgctatc attattattc ctttgctttc gccaggccaa
    14581 gatcttccca aggtagaaat ttatagcgct tttttggcac caccaccgcc accgccgcca
    14641 ccgccaccgc caccagctaa aaagaaaaca tcttctacca gaaccatcaa agctgttcag
    14701 gctactacta gtgttgaacc gggtaaattg gttgcgcctg tagaaattcc tgatgaaatt
    14761 caacaggaag ttatttctga ttttggcatt gaaggtggag ttgaaggcgg agttgagggt
    14821 ggagttatcg gtggagttct cggcggagtt gttgggggag tgattggagg agtgattagc
    14881 caacaaatgg aatccgaaga gccggttcga gcgattggtg aaataaagca accgaaattg
    14941 attcatcgcg ttgagccggt ttatccagaa attgctcgtc aagctcgagt agaagggatt
    15001 gttattttgg aatgcaccac ggatatttat gggcgcgttc aatccgttaa tgttttgcga
    15061 tctgttccgt tgcttgacca ggcagctatt gatgctgtta aacaatgggt ttatgaacca
    15121 atggtaatta atggtcgtcc gagaggagtt gtttttacag ttacagtgaa ttttactatg
    15181 aaaaaatgaa aaataataaa caggcactat ccgtgcctgt taatttttta aatttttggc
    15241 gattttgtct tgtaaaaaaa taaaaatatt ctaatctatc tctaatgata aaagatgaac
    15301 aattagttaa aaattatctt aatggcgatg aagaagcaat tgccatttta ataaatcgtt
    15361 atttaaaaga agtttactta tattttttaa aacgccttaa caattctatt gaggctgaag
    15421 atttaacgca agaaacattt gttaaaatgt ggcgtaattt gaaaaaattt gatcaaaaac
    15481 gtaattttcg aacgtggctt tttgctattg ccaaaaattt attaatagat ttttttcgca
    15541 aacatcagac aaccaatgga ttatccaaag aagttacttt ttcgcaattt attaatcaag
    15601 aagatgaaga aaacgttatt ctggataaga ttatagactg gcaaccatct gccatagagc
    15661 aaattgaaga aaaagaaact cgtgaaaaat tgaggagtat tattaatggt ttgcctctgg
    15721 cacaaaagaa actccttttt tcttacttag aatctgatgt cagctttgaa aaactagctc
    15781 aagaaattaa tatacctact aatactttaa aggcacgcta ttatcgtctg atccaacaaa
    15841 ttaaaaagtt gtttttacaa caataaaaat tcgggagctt tcaatttaag cctcacattg
    15901 cacccaattg gcgcttttta cgtattatct tgtaaggaat atgaataaag taattgccgc
    15961 aaaacacaca aacgaagatg ttctcttaaa aaaaattttg tggaaaatca gggaagaaaa
    16021 aaacaaacgt attcgcaaag aagttttgtt atctttttgt ggtttgatgg tcagtttggc
    16081 gcttttgcca ctttcttttc aattgagtta tagtaatttt attgagacag gattcattta
    16141 ttatttaggc acaattatta tgcattggga gctgtggagc tcttttctaa acgagtttat
    16201 ttttatttta ttagaatcgt tgcccaccgt tgggctcttg ctggttttga ccaatctttt
    16261 catgttagct ttttcattaa aatatttgtt aaaaaattca tctcattttt cttttaaatt
    16321 cttatttagt cgtttaattt agcaattatg aaattggaag atatatctag ttcaaaaaca
    16381 tttaaaatta ttattgggat aatcggcgga ttgatagcga ttcttcttat ttttaaaata
    16441 ggggtagttg ttggtactaa aaaagcctta tttgcttgtc gttgggcaga acactacgct
    16501 cctaattttg tccagcaaag aaatggtata atggataaat ggatgaggga ttttaatgaa
    16561 ggaatagcga tgaagtctca tggggcagta ggaacaatta ttaaaattga taatttagat
    16621 accaacactg cctttttaat cgttaaggga gagaaagaag ttgaaaaggc aattgttgtt
    16681 tctcctgaaa ctattattga aaaatgggaa gagagaatat tgcctgccga tttaagaatg
    16741 ggcgattatg ttgttgttat tggcgatgcc aacgacgaag gtcaaataga agctaaatta
    16801 attagggtta ttcctcatcc gtttccgcct gagcatttgg gtggattgtg attatgtttg
    16861 gagattatag ttagtgctta attaaaataa aaatatgaac aaaattataa attttttaaa
    16921 aaatcataga attatcgcgg tgttgattat tttgataatt gtgagtggcg gctatttttt
    16981 attaaagccc ttgtttttca aaaataccgc agctcaatat ttaactgcta aagtggagag
    17041 aggaaattta actagtagta ttactgttag tggtcaaact attgctgaaa atagtgtaaa
    17101 cttaagtccg aaagcgtcag gagaagttgt ttatgttaat ttagctaatg gcaaacaagt
    17161 tgaagcaggc gaaacgattc taaaaattga cgatgaagat gttcgaagcg aattaaaaga
    17221 tgcgcaaatc gctttgaata atgccaaaga ggagctggct aaattagaag gattatcaac
    17281 tgaagaaggg agcattgaat cattaaaaat taaaagacaa aaagacctaa cgttagctta
    17341 tcaaaacgca gttgataagc tgccagcact ttttaccagc acaacgcctg ctattattac
    17401 aaacctttat aatattttat ttgataataa tttaagtcca gatgatgata atgttgatta
    17461 ttatggaaag actctaatgc tttataatcc agctgctctt caatatagcg atgctactaa
    17521 aaaggcatac gatttagcga agtttagcta tgaggaagcg gctaataatt ataaatcaaa
    17581 aaatattact tattatagtg attccaatga gattgatgag gcaattaatc ttactcatca
    17641 agcgctaaaa aatataaaca atgctgtccg tgctgcttta aatttagtcc atctttataa
    17701 agactttatg atagaagcga aattgcaata tccttcactt gttgatgaac aagatttaat
    17761 gctaactaat tattttgcca ctaccactaa tttatttagc caaattgata ctataaaaaa
    17821 taatattcaa attgctaaag aaaatcttat aaacgtcgat tttgatttaa gcgatcaaaa
    17881 aagattagta gaaaaagccg aacgcaatct taaactcatt caagataaat tagccgatta
    17941 cactgtacta gcgcccttca gcggcatttt aggtaatgtt gccactattt atgtcggtga
    18001 taatgtatcg ccgagtacca ccttgggcgt attagtatct caaggatatg tggtggaggt
    18061 gagtttggct gaaagcgatg ctgctcaagt taaaataggt cagaaggcca atgtttcatt
    18121 tgatgttttg gatgatgttg cggctaccgg taaggtaatt gaaatagata cagtaggcgt
    18181 tgttgtccag ggagtagtga gctataaggc caaaattctt ttggatgtta ttgatgaaag
    18241 agtaaagccg ggaatgagca cgacggtaga aattataact gatgccaaag aaaacactct
    18301 ttttattcct aggacagccc ttaaaaatct gaatggaaaa tattttgtcc gtattttaaa
    18361 agatgatggt acaattgaac aaagagagat aaaaaccggt ttattaactg attcaaaagt
    18421 agaaattatt caaggattga atgaggggga aaaaattatt attgcggatt taaatagcag
    18481 cagtggcgtt aatcttttaa ggactaattt ttctaatcag agaaacaatc aaacacaaat
    18541 accaaggggt attccaggag ctttttagcg ttatgcaaga aaaaaatata attgaagtta
    18601 aaaatttatc caaaatttat gatggcgtgg ttgaaaccat tgctttgagg aatgtaaata
    18661 tgagtattag agaaggcgaa tttgtttcta tcattggccc ttctggatcg gggaagtcaa
    18721 ctcttatgca cattttgggc tgtttagata agtcaaccag cggcgaatat atatttgaag
    18781 aaaaaaatat ttctcatttt agcgaagatg aattggcgga tataaggaaa acaaagattg
    18841 gttttgtttt tcagcagttt aatttattgc ctcgagcaac ggttttacgt aatgttgctc
    18901 tacccttggt ttatgccaat atatctaaaa aagaaagaga agtaagagcg gctaaagttt
    18961 tagaagaaac cgctttccca aaggaattct ggaatcattt acctaatcaa ttatctggcg
    19021 gaatgatgca aagagtggct attgcccgcg cgcttgttaa tgatcctatt ttaattttgg
    19081 ctgatgaacc aactggtaat ttggatagta aaactggaga aaaaatttta gcaactttta
    19141 aagaacttca tcatagaggc agaacaatta ttttagtgac ccatgatcgc tatattgccg
    19201 aacaagccaa aagaattatt tctttaaaag atggggaaat tatagaagat aaaaaaatag
    19261 aagagtccga agaatttaaa attatgagag aataaatatg ttgatatctg atcttttttc
    19321 cgaaacaacg agtgccattc tttctaataa actgcgatca agtttaacta ttttaggtat
    19381 cgttattggc atagggtctg ttattggcat gatctctatt ggaagtggat caaagactaa
    19441 cattgaatca cgattacaat caatgggcac taatatgatt tatgtaatgc caggaatgca
    19501 aacgaatttt tcacccgttt cgcaagggcg tggtacagct caaagcttaa ccattgatga
    19561 tgttaaggct attgaagaga ttccttatgt ttctgatgtt tcgccgattg cgactcggcg
    19621 ttttcaagtt atttcaaaat caagcaatac taatactcaa at
//
LOCUS       DAOSMC010000009        16970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_4031_length_16970_cov_16.036181, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000009 DAOSMC010000000
VERSION     DAOSMC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16970
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4031_length_16970_cov_16.036181"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PL075_01550"
     CDS             complement(<1..254)
                     /locus_tag="PL075_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOD96913.1"
                     /translation="MLKPLGDYILLEPIKEEMVTEAGIVLPETAEQEKPQKGKVIAVG
                     SGRMKDDGTRIPPEVKVGDVVIFHQYGPSEIKIDKKEYLVA"
     gene            complement(360..743)
                     /gene="raiA"
                     /locus_tag="PL075_01555"
     CDS             complement(360..743)
                     /gene="raiA"
                     /locus_tag="PL075_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOD96914.1"
                     /translation="MNINYKIKDVVLTEEMQKDIENLMRTLVERYLLRLDEETNIQID
                     LELSRETHHHLKGNVFYVEANMHLPGRILRAISHAPDIKTGIDEVEEDLKRQIKKYRE
                     KRDSRFRKDWQKLKAFLRGEKEQKF"
     gene            complement(771..1718)
                     /gene="murB"
                     /locus_tag="PL075_01560"
     CDS             complement(771..1718)
                     /gene="murB"
                     /locus_tag="PL075_01560"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOD96915.1"
                     /translation="MATIEFQKNISLATLTTFKIGGPAEYFIRVTNLNELKDAFIKAK
                     EMNKPTFILGGGSNLLASDRGFEGVIIKMQTQEFQVENHNSYFRIICDGGVLLSRVLA
                     LSLEIGATGLEWATGIPGTIGGATFGNSGAYGHSISEVVEKVIAFDPIALEPKEFSQK
                     ECSFDYRESVFKKNGFIITSVYLNLEKNEKGDSKEIVLDYIKERKNKIPPYPSAGCVF
                     KNIPINRASSETLAKIPEEKIKGGKIPVGYLIDQCGLRGERIGDAQISPMHANFIVNL
                     GKATAQDVYQLINLCKTKVQEKFSISLEEEIRYLGNFQN"
     gene            complement(2161..2232)
                     /locus_tag="PL075_01565"
     tRNA            complement(2161..2232)
                     /locus_tag="PL075_01565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2198..2200),aa:Ile,seq:gat)
     gene            complement(2264..2782)
                     /locus_tag="PL075_01570"
     CDS             complement(2264..2782)
                     /locus_tag="PL075_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96916.1"
                     /translation="MADLIKKPKKENSDTINLKEINRKELQKQNLIFIISAVMIIIIL
                     VVIVGLFINFVIKQINYTFEKDSKAVTNLEGFDLKGFAEIKDKLSEPEGEWGNLLETV
                     TTSEELQTSPSSTPTSTIETETEIVVPSENPEIQTSTEEINESTSTTTSTPTPTITST
                     PTSTSTATPPLL"
     gene            complement(2786..3403)
                     /gene="pilO"
                     /locus_tag="PL075_01575"
     CDS             complement(2786..3403)
                     /gene="pilO"
                     /locus_tag="PL075_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOD96917.1"
                     /translation="MAENINQSDKLKKSGLLSRGDKRLLKPLVIYGGIIIIILIAVLV
                     SINQIKKQSAEIKSLRTDYYTLLFESEIYSNLKKNSDILLPYANQVKNLVPEQNRLID
                     FTEAINQLANKNNLELGLIFKEATPSEASKTLFKNIKEAPFTITIKGSFDDFLQFLKD
                     LRTFPYYVDFSNFNIVNLSETISSSEESAKELFSINLDGRVFIKK"
     gene            3538..4650
                     /gene="dprA"
                     /locus_tag="PL075_01580"
     CDS             3538..4650
                     /gene="dprA"
                     /locus_tag="PL075_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOD96918.1"
                     /translation="MTLDCEKIYYNALNIITDCNYGLLSKLKAFYGSFESAWTASNYD
                     DFLNVNPHFALETIENLTSKKGTIDPLKEWEKMKSLEIKMYLPEDDEYPTLLKEIPSF
                     PLAIYQLGDFDQLQATIGVVGTRKPTSYGKMVTEKITKDLVECGFITVSGLAYGIDSV
                     CHQITLENGGSTIAVLGCGLDIIFPPINKRLAQKIIKRGALISEYAPSTPPLKHHFPA
                     RNRIISGLSLGVVVVEAPLKSGALITARFALDQNREVFSVPGSIFSKNSEGTHQLIKS
                     GSKLTSSITDILEELNMIPEIKITSSIDDKIFRADLNIEEQKIIAIIKQADHPILVDE
                     IASLSQLPVSIVNQTLTLLELKNLIKAEANGYRLKA"
     gene            4661..6691
                     /gene="topA"
                     /locus_tag="PL075_01585"
     CDS             4661..6691
                     /gene="topA"
                     /locus_tag="PL075_01585"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOD96919.1"
                     /translation="MAKPKQNLIIVESPAKAKTISRFLNSEYRVESSYGHIRDLPKSK
                     LGIDIEHDFQPTYIIPRKSQKNATKLKKLILKTSKLILATDEDREGEAIAWHLKEVLV
                     PENLKNALPIERIAFHEITQTAIENALKNPRDIDMNLVWAQQARRILDRLVGYLLSPF
                     LWKKMFKGLSAGRVQSVALRLIVDREKEREQFKSQEYWTISADFLSSAQEPFQAELSK
                     INDKILNKLDIKNKDEAEKIAQDLKNAECIIDNITEKLTAKTPPPPFITSTLQQEAWK
                     RLGFSAKQTMTLAQKLYEGIDFGEGSVGLITYMRTDSVVLSEEALNKAKDVITKTFSP
                     NYYYRRQFKNKSRLAQEAHEAIRPTNPSLLPQQLKDKIDNNLFKLYNLIWQRFIASQM
                     APLQIKRLIIEIRANSKNCRYLLKSIGSQIVFDGFSKVYPIKLQEVLLPSLTKNEVVQ
                     LNKVNPVQHFTEPPARYSEATLIKTLEEYGIGRPSTYATIISILKERHYVDRDDNKRF
                     VPSDVGLMVDNLLRTHFPQIVDVGFTAQIEKELDEVAEGKKSWVAVVRNFYEPFKSNL
                     DQKYDEVKKLDVIEETNEVCEKCGAKMVVKYGKFGKFLACSNFPTCRNTKSLNTNQNT
                     HEHCPKCKQGEIIIKKTKRGKIFYGCSRWPDCDYASWTKPEKLEKQNTENEE"
     gene            complement(6703..8004)
                     /gene="ftsZ"
                     /locus_tag="PL075_01590"
     CDS             complement(6703..8004)
                     /gene="ftsZ"
                     /locus_tag="PL075_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOD96920.1"
                     /translation="MKHKNKNKKISQVKKTPQEKDVSKKTQKSSLLKNLKSNVHPRHK
                     KIKNKKGDFLKSSQIINKGIKPSSLEHFPNIKVIGVGGAGGNILSRMKQAKIQGVEFI
                     VVNTDLQDLYKSGIRKKIHIGKNIAHGMGSGMNPEIGRQAAEESRPQLEEAIKGADMV
                     FLTCGLGGGTGSGATPVIADICQQMGILTVAIVTKPFSFEGIKRTQIAEEALLKLKDK
                     VDSYIVIPNDRIFNLIDQTTPLYKAFELIDEILKQAVKGIGDLILSPGIINVDFSDVR
                     TILQNAGMSLIGLGRASGEDRAVKAVKEALNSPLFEYSPHMAKGVLFNVIGDSGLTMT
                     EINEAARLITEVVDPSAKIIFGAMENQRIKKGEIKIMIIAAGFEYSNKFPAHSPFVSS
                     EKNEIKRNNNQQPPIEFKEESAPSLGESLEDIPAFLRKKKK"
     gene            complement(8028..9254)
                     /gene="ftsA"
                     /locus_tag="PL075_01595"
     CDS             complement(8028..9254)
                     /gene="ftsA"
                     /locus_tag="PL075_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOD96921.1"
                     /translation="MALDLGNQNTRLILAQQTKDLKTKILAILERPSDGLNKGSIVDY
                     GAFVNSLFETFEDLKKMGFSPKEAIINVAPPHTTFKIAKVVSGVARNDGEITKNDIDQ
                     LNEKIRETTGETANKKILHIVPRQFSVDDLTNIKDPLGMHGYRLEMEAALIEVFIPHL
                     KTLEKAFTELNKDVSQEVFSPLASSLACLTKKQMSVGTILIDLGAQNTNFIIFEEDQI
                     LDAGILNFGADSITHDIAICLKVPLEVAEKIKLTFGYALSNDINRKESIDLSKVNKEM
                     TNEINKRYLAEIIEARLEEIFDAINAAIKKNNCYAKLAGGAVLIGGGAKLPGIVDFAK
                     KYLKLPAKIGYPINFETTATNEKYSDLIDDPSFVLASGLVIWGMNNIVEKRKINIYHS
                     SSFLQKLKKFFKIFLP"
     gene            complement(9271..9561)
                     /gene="rpsU"
                     /locus_tag="PL075_01600"
     CDS             complement(9271..9561)
                     /gene="rpsU"
                     /locus_tag="PL075_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOD96922.1"
                     /translation="MPIEVTRKSNEPINNFLLRFNRALKQSGVLEEAKTKRFYQPEPN
                     RNQKKESAIYRAQMREKILALKKRGIIKGNEDIKVIKKLLRNPKWSHINLPR"
     gene            complement(9567..9917)
                     /locus_tag="PL075_01605"
     CDS             complement(9567..9917)
                     /locus_tag="PL075_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOD96923.1"
                     /translation="MEKDCVFCKIINKEVPADIIYENERVIVFKDINPKAEVHLLIVS
                     KKHLVDLNETSENDKELLAELFLTAKKVAQQLSLIEKGYKLVLNVGRGAGQVVDHLHL
                     HFLSGSYNHPLNAI"
     gene            complement(9920..11254)
                     /gene="hisS"
                     /locus_tag="PL075_01610"
     CDS             complement(9920..11254)
                     /gene="hisS"
                     /locus_tag="PL075_01610"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOD96924.1"
                     /translation="MGRRPKDYFKNKIKRPKMKTILQAPKGMPDILPEENLLRRKIFK
                     VVEDLANFYGFGQIETPILEETKLFLHGTGPTSDIVQKQMFTLKTPGKDFLALRPEFT
                     PNIVRAYLENGLYNNPKPVKLFAWGPVFRYEQPQRGRSRQFWQCNFEILGSADPIYDA
                     LTMQLFYNLFTELKLKNLIIDINTLGCDNCRPLWHKNLKNYFRSKRRFLCTDCKNRLS
                     TNPLRILDCKNESCQSLKNGAPKMIDFLCPECRKHFKQVLEYLEILGLPYNINHYLVR
                     GLDYYTKTVFEIRLVDDNLALCGGGRYDYLVEMLGGKPTPAVGGAAGIERLVEVLKKN
                     NYKVENTFKPKVFLIAIGDLAKQQALKYLETLRKVKIPAAESFAHESLKAQLRQADKA
                     EIPFVIIIGQKEAMDDTIILRDMKASTQEVIVGEKMVDELKKRFKNDNIANA"
     gene            complement(11263..11823)
                     /gene="lepB"
                     /locus_tag="PL075_01615"
     CDS             complement(11263..11823)
                     /gene="lepB"
                     /locus_tag="PL075_01615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOD96925.1"
                     /translation="MRQFRQFLANLWSTIEVVVIAIAVVLIIRTFLFQPFIVEGASMF
                     PNFQNSDYLIVDELTYRLRAPMRGEIIVFHPPQSPGNYYIKRIVGLPGETVEIKEGKV
                     IIYNTEHPEGFVLKEEYLLNSKTPGEEKIILGPDEYFVLGDNRSASYDSRSWGPLKKD
                     KIVGLVRLQLWPLSSAKAFSTVDYRY"
     gene            complement(11922..14759)
                     /locus_tag="PL075_01620"
     CDS             complement(11922..14759)
                     /locus_tag="PL075_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96926.1"
                     /translation="MKNHDKRTENLREKIQRWKKGELTIDHFTSDERIELLEMHEISQ
                     EEFLSHLPKRLRDYWQAISQLLEKKRIFRNVEAAEALPLNVKGKYQRLNMSRGILAKF
                     KEWGLLETLRIQNHNVYFCALPGFYSVKELLELIPKEYQKSILELMPDQWLKMNELAE
                     TLNISPRFLEEILTAFLSKGWLVKSKSGYKKSKVLEEAGISVESLKSLELVELHNLLA
                     EEQELKGAGAIPQNLSELKETIQKDIEQKTVQPLISLFDGESIRLTFLGEVRFGHQFA
                     DMKLIEWGLEMLKQYLPHYIITSDFVQGDFRGIQVERLRSLTRTGFLHRIETQHKAAN
                     MLLEELESIAQEKVIYQLSDDDWQVALSRVIIALEQYRNIRKLGATGFWPEEIKRRSG
                     ADYYRLMKIQWETIQPYMYRIGRSLLNAEETEKVIGNRISELLVIIFILLFEQFNQPI
                     PQKYKKLVDLEALHGDKANSKRIVTPDPVHLELHAFNKKILAQHNIAFSDITQYIDTG
                     FIPESILRHLQASGKETPYLWVDFHQERFWGTKVFNTFIFNLPGCQNPSLAAEGQIKA
                     FHSKVLTDKAHRQITVRKEPPTPGIISFEIFKDGRLKISLLNNKVKKVIEAQKDQAEK
                     KDILVYTSDWQLGSLTMRPEWGIKFLDYALYSRKGNVLILNGDIIQGFHYPRFVSENR
                     PKRLVSVSSQKAFTLRILEPLFPAPNLEAMDLLLGNHEAEIWGADLTGQNNLEFLFFA
                     LTELYNQQKKFNALEKYPDIKIWSRIRNTQTAAPGGAIINHPFAGRQLKSGFKYVVQH
                     QFYPRGAGRTPLHEQMRWVRNIAKSIADAHILLGGHKHTFWIAQIAELIMLQLPGFND
                     QSAFEFARGLMPEPMGCIIEFSNQSGITIELIPIEFLEAYKCVSPAYKDIDAQGLLDR
                     PKPGTREYQYGFDSSFIYELEETVDAEYQEV"
     gene            complement(14909..15550)
                     /gene="pcm"
                     /locus_tag="PL075_01625"
     CDS             complement(14909..15550)
                     /gene="pcm"
                     /locus_tag="PL075_01625"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOD96927.1"
                     /translation="MSIQQDKLIEDLVKDGFLHDPRVIEAFKKIDRSNFVIEPLKNNA
                     YINSPLAIGFGQTISQPLTVAFMLEKLDVQPGHKVLDVGSGSGWTTALLAEIVGEKGK
                     VYGIEIIPEIYEFGKANVMRYSFLNKGIVEMYCGNGLEGLKEAAPFDRIMCSAAVKEI
                     PPAFKDQLKIGGKMIFPMNNSLWLMSKKNETDFDTEEYPGFIFVPAIEKILDL"
     gene            complement(15600..16913)
                     /locus_tag="PL075_01630"
     CDS             complement(15600..16913)
                     /locus_tag="PL075_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOD96928.1"
                     /translation="MQLINGKKKNNVTMQTKISFLTKKGLNLLLIPLKESTNVTFLIL
                     VKAGSEYESINEAGISHFLEHMCFKGTEKRPSPNILSSELDALGASYNAFTGREYTGY
                     YAKAAVEHTDKILDIVTDIYLNSLFNDQEIEKERGVILEEKNYYEDTPSIKIEDLIFE
                     LLYPHQAAGRPIIGREEVIKKVNHKDFVRYFQKHYTNKKTLIVIAGNFQPTQIIAKVC
                     ALMKNIRKGEGGKKFKTSYKQKEPAVLIQPKNTDQNHLILAARTFNCFDKRRYILSVL
                     STILGSGMSSRLFQRIREEMGAAYYIHSFTDLSLDHGFLGIAGGLAVDKTEAALKVIK
                     SELDKLTEKIVDSNELNKAKSYLKGKLALSVETSNDYAFYFGSQQLFYNRTFSLEEIY
                     QKIDEVDQNSILQLAKHIFHPSNLNLAIIGPYSLKDQPRFKKLII"
BASE COUNT         5380 a         3098 c         2648 g         5844 t
ORIGIN      
        1 gcaacaagat attctttttt atcaattttt atttccgaag ggccgtattg atgaaaaata
       61 acaacgtcac ccactttaac ctctggggga attcttgtgc catcatcttt catgcgacca
      121 gagcccacag caatcacttt acctttttgt ggtttttctt gttctgctgt ttcgggcaaa
      181 acaataccgg cctccgtcac catttcctct ttgattggtt ctaaaagaat ataatctcct
      241 aatggtttga gcatattttt aaaatttttt aaattggcac tcccgacctt taagtgctaa
      301 ttaatattat tttatttttt gtttactatt tgtcaagaga ttatcttgaa taattcaatt
      361 taaaattttt gctctttttc tcctcttaaa aaagctttta atttttgcca atcttttcga
      421 aaacgagagt ctcttttttc tctatatttt ttaatctgac gctttagatc ttcctctacc
      481 tcatcaatac cagttttaat atctggggca tgtgagatgg cgcgtaaaat tctcccggga
      541 agatgcatgt tcgcttcaac ataaaaaaca ttgcctttta aatgatggtg cgtttcgcga
      601 cttaattcta gatcaatctg aatatttgtt tcctcgtcta atcttaaaag atatctctct
      661 accaatgttc tcattaaatt ttcaatatct ttctgcattt cttcggttag aactacgtct
      721 ttaattttat aattgatatt cataataatt ttttcttttt accgacgatt ttaattttgg
      781 aaattgccaa gatagcgaat ctcttcttca agagaaatag aaaatttttc ttggactttt
      841 gttttacaaa gattaattaa ttgataaacg tcttgggctg ttgccttgcc caaattcaca
      901 ataaaattag catgcattgg tgaaatttga gcatcgccga ttctttctcc tcttagaccg
      961 cactgatcaa ttaaataacc cacggggatt ttgccacctt taattttttc ttctggaatt
     1021 ttcgctaaag tttctgagga agctcgatta atcggaatat ttttgaaaac acatcccgct
     1081 gaaggataag gcggaatttt atttttcctc tctttaatat aatcaagaac aatttcttta
     1141 gaatcaccct tttcattttt ttctagattt aaataaacgg aagtaataat aaaaccattc
     1201 tttttaaaaa cgctttcccg ataatcaaaa gagcattctt tctgcgagaa ttcctttggc
     1261 tctaaagcaa ttggatcaaa agcgataact ttttctacta cttcggaaat actgtgacca
     1321 taagcgccac tattaccaaa agttgctccg ccgatagttc cgggaatacc cgttgcccac
     1381 tctaatcctg tagcacctat ttctaaactt agtgccaaaa cgcgtgataa taacactcct
     1441 ccatcgcaaa ttattctgaa ataggaatta tgattttcaa cttgaaattc ttgagtttgc
     1501 atttttataa tcactccctc aaaacctcta tcgcttgcta acaaattaga cccaccgcct
     1561 aaaataaaag ttggcttgtt catttctttg gctttaataa atgcatcttt taactcattt
     1621 aaattagtaa cacgaataaa atattcggcc gggccgccga ttttaaacgt agtaagtgtt
     1681 gccagagaaa tatttttttg aaattcaatc gtagccataa cgaagcgaga tgtattgaac
     1741 tcagtttcat tctggtccga aggcggttga ctccaattac catttaagtg agtgctcaat
     1801 cctccggacc agaatgaaac caaatccttc tttattataa tcaaaattga ttatctggca
     1861 aatcaaaata atcaaaattg taagactata aattaaaaag aaatccgtcc ttttcaggac
     1921 ggatttccac ggtaattgga tttcctaaat gactgaacat tcaggaaaca cactcacttc
     1981 atcttctatt atactcaaat atattcatta gtcaagacct cttttgtcgc ttttctttaa
     2041 accacaggga ttggatgatt aaaaacttgc ctaattttat tcggagtggt aagaggtgct
     2101 gcggcagcga tatttctaaa gaaatatcgc tgccgcagca cgaatgagtt ttccattctg
     2161 tgcgcatggg agggatcgaa cctccgacct ctgtcttatc aggacagcgt tctaccactg
     2221 aactacatgc gcgtttttta aaaaattact ggcgaaagca aaattaaaga agtggtggcg
     2281 tggccgtgga agtagaagtg ggcgtggagg taatggtagg ggtaggagta gaggtggtgg
     2341 tagaggttga ttcattaatt tcttcagttg atgtttgaat ttcaggattt tcgcttggca
     2401 caactatttc agtctcagtt tctatcgtag atgtcggagt tgaagaagga gatgtctgaa
     2461 gttcttccga cgttgttacg gtctctaata aatttcccca ctccccttct ggttctgata
     2521 atttatcttt tatctcggca aatcctttta aatcaaaccc ttctaaatta gttactgcct
     2581 tagaatcttt ttcaaaagta taattaattt gtttaataac aaaattaata aacaacccta
     2641 ctattacaac cagaattatt attatcataa ccgccgaaat aataaaaatc aagttctgtt
     2701 tttgaagttc ttttcgatta atttctttaa gattaatcgt atcgctattt tccttttttg
     2761 gtttttttat taaatcggcc attttttatt ttttaataaa gacgcgacca tccaaattaa
     2821 ttgagaataa ttctttagcg gactcttccg acgatgaaat agtttctgat agattaacaa
     2881 tattaaaatt gctaaaatct acataataag gaaacgttct taaatccttt aaaaattgaa
     2941 gaaaatcatc aaaacttccc ttaatcgtta tcgtaaatgg cgcttcttta atatttttaa
     3001 acaaggtctt gcttgcttcc gagggcgttg cttctttaaa aattaatcct aattctaaat
     3061 tatttttatt agctaattga ttaatggctt ctgtaaaatc gattaaacga ttttgttcag
     3121 gaaccaaatt ttttacttga ttagcataag gaagaaggat gtcgctattc ttcttcaagt
     3181 tggaatagat ttcggattca aatagtaaag tataataatc agtacgcaat gattttattt
     3241 cagcgctttg tttttttatt tgattgatcg aaactaatac ggcgataaga ataataatta
     3301 taattcctcc gtaaataacc agtggtttca aaagcctttt atctcctcgg cttaaaagac
     3361 ctgatttttt aagtttatcc gattgattta tattttccgc cataatattg tattaaaact
     3421 ctatagttat attataaaaa agagtaaaat aaaaaacaag gaagacctcg ctttttccag
     3481 cctattgtca aaaagagatg attataataa aatagtatga taaaaatata atagaatatg
     3541 actttagatt gcgaaaaaat ttattataat gccttaaaca ttattactga ttgtaattat
     3601 ggattgcttt ctaaattaaa agcgttttat ggttcttttg aatcagcttg gactgcttca
     3661 aattatgatg attttttaaa cgttaatccg cattttgctt tagaaactat agaaaatctt
     3721 accagtaaaa aagggacaat agatcctctg aaagaatggg agaaaatgaa aagtctagaa
     3781 ataaaaatgt atttgcctga agacgatgaa tacccgacat tattaaaaga aatcccctct
     3841 tttcctctgg ctatttacca attaggtgat tttgatcagc ttcaagccac cattggcgtt
     3901 gtcggcacaa gaaagccaac aagttatgga aaaatggtaa ctgaaaaaat aacaaaggat
     3961 ttagtagaat gcggatttat tactgtaagt ggtcttgcct acggcatcga ttctgtgtgt
     4021 catcaaataa cattagaaaa tggcggatcc actattgctg ttttgggatg cggattagat
     4081 attatttttc ctccaattaa taagcgtctc gctcaaaaaa taataaaaag aggtgctttg
     4141 atttctgaat acgctccttc tactccaccg ttaaaacatc attttccagc tcgcaacaga
     4201 ataattagtg gattgtcttt gggtgtggtt gtggtagaag ctccattaaa atccggcgct
     4261 cttattactg cgcgttttgc tttagatcaa aatcgagaag ttttttctgt tccaggatcg
     4321 attttttcta aaaattccga aggaactcat caattaatta aatcaggttc taaattgaca
     4381 agttcaatta cagatatttt ggaagaactg aatatgattc ccgaaattaa gattacttct
     4441 tctattgatg ataaaatttt tcgcgctgac ctaaatattg aagaacaaaa aattattgct
     4501 attataaaac aagcagacca tcccatttta gtagacgaaa ttgcttcttt aagtcaatta
     4561 cctgtgagta tagtgaatca aacgctcact cttttagaac taaagaatct tattaaagcc
     4621 gaagcaaatg gttatcgatt aaaagcttaa atgatcagca atggctaagc caaaacaaaa
     4681 tcttattatt gttgaatcac ctgctaaagc taaaacgatt agccgttttt taaactctga
     4741 gtatcgcgtt gaatcttctt atggacatat tcgcgatttg ccgaaatcaa aattaggcat
     4801 tgatattgaa catgattttc aacccactta tattattccg cgcaaaagtc agaaaaatgc
     4861 cactaaactt aaaaaattaa ttcttaagac ttcaaaactt atcctagcta ctgatgaaga
     4921 tcgagaagga gaagctattg catggcatct caaagaggtt ttggttcccg aaaatttaaa
     4981 aaatgccttg cctattgaaa gaattgcctt tcatgaaata actcaaacag ccatcgaaaa
     5041 tgctttgaaa aatcctcgcg atattgatat gaatctcgtt tgggctcagc aagcgagacg
     5101 aattttggat cgtctcgttg gttatctatt atctccattt ctttggaaaa aaatgtttaa
     5161 aggattatct gcgggacgcg tccaatcggt agctttacgt ttaatcgttg atagagaaaa
     5221 agaacgagaa caatttaaat ctcaggaata ttggactatc tccgctgatt ttctttcctc
     5281 tgcccaagag ccttttcaag cagagctttc taaaattaat gataaaattt taaataagct
     5341 ggatattaag aataaggacg aggctgaaaa aatagctcaa gatctaaaaa acgccgagtg
     5401 tatcattgat aatattactg aaaaattaac ggcaaaaact cctccgcccc catttattac
     5461 aagcactctt caacaggagg cttggaagcg attaggcttt tcagcaaaac aaacaatgac
     5521 tttagctcaa aaattatacg aaggaattga ttttggcgaa ggttctgttg gcttaattac
     5581 gtatatgaga accgattcgg tagttttgtc agaagaagca ctaaataaag ctaaagatgt
     5641 tattactaaa acattttctc ctaattatta ttatcggaga caattcaaaa ataaatcacg
     5701 gcttgctcag gaagcccatg aagctattag accgactaat ccttcgcttt tgcctcagca
     5761 attaaaagat aaaattgata ataatctttt taaactttat aatctcatct ggcaacgttt
     5821 tattgcttct caaatggcac cccttcaaat taagcgttta attattgaaa ttagagcaaa
     5881 tagtaaaaac tgcagatatc ttttaaaaag cattggtagc caaattgttt ttgatggctt
     5941 ttcaaaggtt tatcccatta aacttcaaga agttttatta ccttctttaa caaaaaacga
     6001 agttgtgcag ctgaataaag ttaatcctgt tcaacatttt actgaaccgc cggcacgcta
     6061 ttcagaagct actcttatta aaacacttga agaatacggc attggtcgac cttcaacata
     6121 tgccaccatt atttctattt taaaagaacg ccattatgta gatcgcgacg ataacaaacg
     6181 attcgttccc agcgatgttg gtttaatggt tgataatctt ttacgcaccc actttcctca
     6241 aattgttgac gtcggtttta ctgctcaaat agagaaagaa ttagatgaag ttgccgaagg
     6301 taaaaaaagt tgggttgcag tagtaagaaa tttttatgaa ccttttaaat ctaatttgga
     6361 tcaaaaatac gatgaagtca aaaaattgga cgtgatagaa gaaacaaatg aagtttgcga
     6421 aaaatgtgga gcgaaaatgg ttgtcaaata tggtaaattt ggtaaatttt tggcctgttc
     6481 taatttcccc acctgtcgca acacaaagag tttgaatacg aaccaaaata cacacgagca
     6541 ttgtcctaaa tgcaaacaag gagaaattat tattaaaaaa accaaacgag gaaaaatttt
     6601 ttatggttgt tctcgttggc cagattgtga ttatgcttct tggactaagc cagaaaaatt
     6661 agaaaaacaa aacacagaaa acgaagaata aaaagagtaa ttttatttct ttttctttct
     6721 taaaaaagca ggaatgtctt cgagggattc tcctaaagaa ggcgcgcttt cctctttgaa
     6781 ttcaataggc ggttgctgat tattattccg tttgatctca tttttttcag aagaaacaaa
     6841 aggagaatga gcaggaaatt tatttgaata ttcaaaacca gcagcaataa tcataatttt
     6901 aatctctcct ttttttatgc gttgattctc cattgcacca aaaataattt tagctgaagg
     6961 atcaaccacc tcagtaataa gtcgtgccgc ttcgttaatt tcagtcatcg ttagccctga
     7021 atcacctatg acattaaaaa gaactccttt ggccatatga ggagaatatt caaacaatgg
     7081 agaattaaga gcctccttaa cggccttaac agctcgatct tccccgctgg ctcgtcctaa
     7141 tccaatcaaa ctcatcccgg cattttgtaa gatagttcta acatcagaga aatccacatt
     7201 aataatgcct ggtgaaagaa taagatcccc aattccttta actgcttgtt taagaatttc
     7261 gtcaattaat tcaaaggctt tatataaggg cgtggtttga tcaatcaaat taaaaattcg
     7321 atcattggga ataacaatat aagaatcgac tttatccttt agtttaagaa gtgcttcttc
     7381 agcgatttgc gtccttttaa ttccttcaaa agaaaaaggc tttgtaacaa tagcgactgt
     7441 taaaataccc atttgttggc aaatatccgc aataacaggc gtagctcccg aaccagtgcc
     7501 acctcctaat ccacacgtta aaaaaaccat atcagcgcct ttaatggctt cctcaagttg
     7561 gggccgtgat tcttctgcgg cctgacgacc aatctctgga ttcattcccg accccattcc
     7621 atgagcaata tttttcccaa tatggatttt ttttctaata cctgatttat ataagtcttg
     7681 taaatcagta ttaactacaa taaattccac tccttgaatt ttggcttgct tcatgcgtga
     7741 taaaatattt ccaccagctc ctccaacgcc aataacttta atattgggaa agtgttctaa
     7801 cgaagaaggt ttgatgcctt tatttataat ttggctgctt ttcaaaaaat ctcctttttt
     7861 attcttaatt tttttatgac gagggtgaac gttgctcttt aaatttttaa gcaaagagct
     7921 tttttgtgtt tttttgctaa catctttttc ttgaggagtt tttttaacct gggatatttt
     7981 tttatttttg tttttatgtt tcatatttat ttatttttac ggctatttta cggtagaaag
     8041 attttaaaaa atttttttaa tttctgcaag aaagaactgc tatgataaat gttaatttta
     8101 cgcttttcta ctatattatt cattccccaa ataactaatc ctgaagctaa aacaaaagat
     8161 gggtcatcaa taagatccga atatttttcg ttggtcgctg ttgtttcaaa attaatagga
     8221 taacctattt tagccggtaa tttaaggtat tttttagcaa aatcaacgat accaggtaat
     8281 ttggcgccgc caccaatcaa aactgctcct ccagctaact tagcataaca attatttttt
     8341 ttaatagcag cattaatagc gtcaaaaatt tcttctaaac gagcttcaat aatctctgca
     8401 agataacgtt tattgatttc atttgtcatt tctttattaa ccttagataa atctatactt
     8461 tctttgcgat taatatcatt ggacagggcg tatccgaaag ttaatttaat cttttccgct
     8521 acttccaatg gcacttttaa acaaatagca atatcgtggg taatgctgtc agctccaaaa
     8581 tttaaaattc ctgcatctaa tatttggtct tcttcaaaaa tgatgaagtt tgtattttga
     8641 gctccaagat caatcaaaat ggtcccaacg ctcatttgtt ttttagttaa acaggcaaga
     8701 gatgaagcca agggactaaa aacttcttga gagacgtctt tatttaattc agtaaaggcc
     8761 ttttccaagg tttttaagtg aggaataaaa acttcaatta gagctgcctc catttccaaa
     8821 cgataaccgt gcattcctaa gggatcttta atgttggtta aatcgtcaac gctaaattga
     8881 cgaggaacaa tgtgtaaaat ttttttattg gctgtttcgc cagttgtttc tcgaattttt
     8941 tcattcaatt gatcaatatc attttttgtt atttcaccat cattacgagc taccccgcta
     9001 actactttgg caattttaaa tgtggtatgt ggcggcgcta cattaataat tgcttctttt
     9061 ggtgaaaacc ccattttttt aaggtcttcg aacgtttcaa ataaagaatt aacaaaggct
     9121 ccataatcaa cgatagagcc tttatttaat ccatcggatg gtctttctaa tatcgccaga
     9181 atttttgttt tcaaatcctt cgtttgttga gctaaaatta aacgagtatt ttgatttcct
     9241 agatcaagtg ccaaaatcgg cttcattttt ttatcggggt aaattaatat gagaccattt
     9301 aggattcctt aaaagttttt ttatcacttt aatatcttcg tttcctttga taatgcctcg
     9361 tttttttaag gctaaaattt tttccctcat ttgtgctcga tagattgctg actccttttt
     9421 ttgattacga ttaggctcgg gctgataaaa tcttttcgtt tttgcctctt ctaaaacacc
     9481 ggattgtttt aaagcccggt taaatcttaa aagaaaatta ttaattggtt cattggattt
     9541 tcttgtaact tctataggca taaaaattaa attgcattga gaggatgatt ataacttcca
     9601 ctcaaaaaat gaagatggag atgatcaact acttgacccg ctccccgacc gacgtttaaa
     9661 actaatttgt aacctttttc aataagactt aattgctgag ctactttttt cgcggttaaa
     9721 aatagttcgg ctaataattc tttatcgttt tcgctagttt catttaaatc caccagatgt
     9781 tttttagaaa cgatcagtaa atgaacttcg gctttgggat ttatatcttt aaaaacaatt
     9841 actctttcat tttcataaat aatatccgct ggcacttctt tatttataat tttacaaaac
     9901 acacaatctt tttccatatt taagcatttg ctatattatc atttttaaat ctttttttca
     9961 attcatcgac cattttttca ccaactataa cctcttgtgt gcttgctttc atatctcgca
    10021 aaattattgt atcatccatt gcttcttttt gcccaataat aataacaaaa ggaatctccg
    10081 ctttgtctgc ttgtcgtaat tgagctttta atgattcgtg agcaaaagat tcagcggccg
    10141 gtattttaac ttttcttagt gtttctaaat atttcaaagc ttgttgtttg gccaaatcac
    10201 caatagcaat taaaaaaacc ttcggcttaa aagtgttttc tactttataa ttattctttt
    10261 ttaatacttc taccaatcgc tcaattccgg cagctcctcc tacagctggc gtgggctttc
    10321 caccaagcat ttctactaaa taatcatagc ggcctcctcc acacaaagcc aaattatcat
    10381 ctactaatcg aatttcaaaa acggtttttg tataataatc aagccctctt actaagtagt
    10441 gattgatatt ataaggaagg cctaaaattt ctaaatattc aagaacttgt ttaaaatgtt
    10501 tgcgacattc ggggcaaaga aaatcaatca tttttggagc gccatttttt aatgattgac
    10561 aagattcgtt tttacaatcc aaaattctta aaggattggt tgataatcga tttttacaat
    10621 cagtgcataa aaatcttctt tttgacctaa aataattttt taagttttta tgccagagag
    10681 gacgacaatt atcacaaccg agagtgttga tatcaattat caaatttttt aatttcaatt
    10741 cggtaaataa attataaaat aactgcattg ttaaagcgtc gtaaatagga tcagcagacc
    10801 ccaaaatttc aaaattgcat tgccaaaatt gacgacttct tcctctctga ggttgttcat
    10861 agcgaaaaac tggcccccaa gcaaacaatt tgactggctt aggattgtta tataatccat
    10921 tttctaaata ggcacgaaca atattaggag taaactccgg ccgcaatgct aaaaaatctt
    10981 tacccggagt ttttaaagta aacatttgct tttgaacaat gtcacttgtt ggtccagtgc
    11041 catgcaaaaa taattttgtt tcttctagga ttggagtttc aatttgacca aaaccataaa
    11101 aattcgctaa atcctctact actttaaaaa tttttcttct taataaattc tcctcaggca
    11161 aaatatcagg cattccttta ggcgcctgta agatggtttt catttttggt cttttaattt
    11221 tatttttaaa ataatctttt ggtcttcttc ccatattttt aattaatatc tatagtcaac
    11281 ggtcgaaaag gcttttgctg aagaaagtgg ccataattgc aatcgaacaa gccctacaat
    11341 tttatctttt tttaacggtc cccacgaccg cgaatcatag ctggctgaac ggttatcacc
    11401 caaaacaaaa tattcgtccg gccctaaaat tattttttct tctccgggcg ttttgctatt
    11461 taaaagatac tcctctttaa gtacaaatcc ttccggatgc tcggtattat aaataataac
    11521 ctttccttct ttaatttcga ctgtttcgcc gggtaaaccg acaattcttt tgatataata
    11581 atttcctgga ctttgaggtg gatgaaatac aattatttct cctctcattg gcgcacgaag
    11641 acgatacgtt aattcatcaa caataagata atcagaattt tgaaaattgg gaaacatgct
    11701 tgcgccttca actataaatg gttgaaataa aaatgtgcga ataatcaaaa caaccgcgat
    11761 tgcaataacc actacttcaa tagtactcca caaattagct aaaaattgcc gaaattgtcg
    11821 catagaatta attttgataa ttctattata aacaaaaatc ttaaaaaatc aaataaaaag
    11881 agaggatctt gtgaccctct ctattaattt ttaattttca atcaaacttc ttgatattct
    11941 gcatcaaccg tctcttctaa ctcataaata aacgatgaat caaagccata ttgatattct
    12001 cttgttccag gtttgggacg atcaagcagt ccctgagcat caatatcttt ataagctgga
    12061 gaaacacatt tgtaggcttc caaaaattca ataggaatta attcaatggt tatgccgctt
    12121 tgatttgaaa actcaataat gcaacccatt ggttcaggca ttaatccccg agcaaattca
    12181 aaagctgact gatcattaaa tccgggcaac tgcaacataa tcagttcggc aatttgagca
    12241 atccagaaag tatgtttgtg tccgcccaaa aggatgtgag catcagcaat tgatttcgca
    12301 atatttctta cccatctcat ttgttcgtga agcggtgttc tcccggcacc acgaggataa
    12361 aattgatgtt gaacaacata tttaaatcca cttttcaatt gacggccagc aaaaggatga
    12421 ttaataattg ctcctccagg agcagcggtt tgagtatttc gaattcgcga ccagattttt
    12481 atatctggat atttttctaa cgcattaaat tttttctgct gattatataa ttcagtaaga
    12541 gcaaagaaca ggaattcgag attattctgt ccagtcagat cagcgcccca gatttctgcc
    12601 tcatgatttc ccaataaaag atccatagct tcaagattag gggctggaaa gagtggctct
    12661 aaaatacgta aagtgaaagc tttttgactg ctaaccgaaa ccagcctttt tggtcgattt
    12721 tctgaaacaa agcgtggata atgaaatcct tgaataatat caccgttgag aataagaaca
    12781 tttccttttc ggctataaag agcgtaatct aaaaatttta ttccccactc aggtctcatt
    12841 gttaatgaac ctaattgcca atcggaagta taaactaaaa tatctttttt ctcagcttgg
    12901 tctttttgag cttcaataac ctttttgact ttattattta gcaatgaaat ctttaatctg
    12961 ccatctttaa aaatctcgaa actaataata ccaggagttg gcggttcttt acgaacagtt
    13021 atttgacgat gtgctttatc agttaaaact ttgctgtgaa aggccttgat ttgaccttca
    13081 gctgctaaac taggattttg acagccgggt aaattaaaga taaaggtgtt gaaaactttt
    13141 gtgccccaaa aacgttcttg gtgaaaatca acccaaagat aaggagtttc ttttccactc
    13201 gcttggagat gtcttagaat cgattcagga ataaatccgg tatcaatata ttgagtaata
    13261 tcactgaagg caatattgtg ttgagcaaga atcttcttgt taaaagcatg cagttctaaa
    13321 tggacgggat cgggcgttac aattctttta gaattggctt tatcgccatg gagtgcttca
    13381 agatcaacta attttttgta tttttgagga atcggctgat taaattgttc aaaaagtaaa
    13441 ataaaaataa tcactagcaa ttctgaaatt cgattgccaa ttactttttc tgtttcttcg
    13501 gcatttaata aggaacggcc tattcgatac atatagggtt gaatagtttc ccactggatt
    13561 ttcattaagc gatagtaatc ggccccggaa cgtcttttaa tttcttccgg ccaaaaacca
    13621 gtagcaccta atttgcggat attacgatat tgttcaaggg caataataac tcgtgaaagc
    13681 gccacttgcc aatcatcatc tgaaagttga taaataactt tttcttgagc aattgattct
    13741 aattcctcta acaacatatt tgctgctttg tgttgggtct caattcgatg gagaaatcct
    13801 gtacgtgtta acgaacgcaa tctttctacc tggataccac gaaaatcacc ctgaacaaaa
    13861 tcagaagtaa taatgtagtg aggtagatac tgcttaagca tctcaagtcc ccattcaatc
    13921 aatttcatat cggcaaactg atggccaaat cttacttcgc ctaaaaatgt aagacgaatg
    13981 gattcgccat caaaaagcga tattaacggc tgaacggttt tttgttcaat atctttttga
    14041 attgtttctt ttaattcaga taaattttga ggaatggcgc cagcgccttt taattcttgt
    14101 tcttctgcta ataaattatg aagctctact aactcaagac tcttaagcga ttcaacacta
    14161 atgcctgctt cttctaacac cttacttttt ttataaccgc ttttactttt aactaaccag
    14221 ccttttgata aaaaagcagt aagaatttcc tctaaaaaac gagggctaat attaagtgtt
    14281 tctgccaatt cattcatctt taaccattga tcgggcataa gctcaagaat gcttttttga
    14341 tattctttag ggatcaattc tagcaattct ttaaccgaat aaaatcccgg taaagcacaa
    14401 aaataaacat tgtgattttg aatccgcagc gtttcaagta atccccattc tttaaatttg
    14461 gcaagaatac cccgcgacat gtttaagcgt tgatatttgc ctttgacgtt taagggaaga
    14521 gcttcggctg cttcaacatt tcgaaaaatt ctttttttct caagcaattg actaatagct
    14581 tgccagtaat cccgtagcct tttgggcaaa tgactaagaa attcttcttg actaatttca
    14641 tgcatttcta gtaattcgat tctttcgtca gaagtaaaat gatcaatcgt taattcaccc
    14701 tttttccacc tttgaatctt ttctctcaaa ttttcagtcc tcttgtcgtg atttttcatt
    14761 gaatgatcct cctttgttaa attattaaag atctatgtca atggaagatt ttttaattct
    14821 tccgatttca ttatatatct agatattttt ttatcaaata gcaatgtcca tcgtttaaaa
    14881 atttttaaac ccctttgggg ggtttaaact ataaatctaa aatcttttca atagctggca
    14941 caaaaataaa tcctggatat tcttcggtat caaaatcagt ttcatttttt ttactcataa
    15001 gccaaaggct attgttcatt ggaaaaatca ttttaccacc gatttttaat tgatccttaa
    15061 aagctggggg gatttcttta actgcagctg aacacataat gcggtcaaat ggagctgctt
    15121 ccttcagccc ctcaaggcca tttccacaat acatttcaac aatccctttg ttaagaaaac
    15181 tataacgcat cacattggct ttaccaaatt cgtatatttc aggaataatt tcaattccat
    15241 acacttttcc tttttctcca acaatttcag ccaataaagc cgttgtccag ccggaaccag
    15301 atccaacatc taaaacctta tggcctggct gaacatctag tttttctaac ataaaagcta
    15361 cagttagagg ctgagaaata gtttgcccaa agcctattgc caagggtgaa ttaatatagg
    15421 cattattttt aagaggctca attacaaaat tagagcgatc gatttttttg aaagcttcaa
    15481 tcacgcgcgg atcatgtaaa aaaccatctt ttaccaaatc ttcaattaat ttatcttgtt
    15541 gaatgctcat agctttgacc ttagcgactt tattaatcca tcttcattat aattatatat
    15601 caaataatta attttttaaa tctaggttga tcctttaaag aataaggacc aataatcgct
    15661 aaattaagat ttgatggatg aaaaatatgc tttgctaatt gcaaaataga gttttgatct
    15721 acttcgtcaa ttttttgata aatctcctca agagagaacg ttctattata aaaaagctgc
    15781 tgagagccaa aataaaaggc gtaatcgtta gaagtttcca cagaaagagc tagtttacct
    15841 ttaagataac ttttggcctt gtttagttcg ttggaatcta caattttttc agtaagttta
    15901 tctaattcac ttttgatgac ctttagagcc gcttctgttt tatccacagc caacccacct
    15961 gcaattccta aaaaaccatg atctaaactt aaatcagtaa agctgtgaat ataatatgcc
    16021 gcacccattt cttctcgaat tctttggaaa agccgtgaac tcattccact gcctaaaata
    16081 gttgaaagca ctgataaaat ataacgtctt ttgtcaaaac aattaaaagt tcgggccgca
    16141 agaattaaat gattttgatc agtatttttt ggttgaatta aaacggctgg ctctttttgc
    16201 ttgtaactgg ttttaaattt ttttcctccc tctccttttc taatgttttt catcaaagca
    16261 caaacttttg caattatttg tgttggttga aaatttcccg cgatcacaat taaagttttt
    16321 ttattagtat aatgcttctg aaaataacgt acaaaatctt tatgattaac ttttttaata
    16381 acctcttccc taccaataat tggtcgacca gctgcctgat gaggatataa taattcaaaa
    16441 attaaatctt caattttaat actgggcgta tcctcgtaat aatttttttc ttctaaaatt
    16501 acgcctcgct ctttttcaat ttcttgatca ttaaacaaag agttaagata aatatccgtg
    16561 acaatatcta aaatcttatc ggtatgttct actgctgctt tggcataata accggtatac
    16621 tctcgaccag tgaaagcatt gtaagatgcg cccagcgcat ctaattcaga acttaaaata
    16681 ttaggactgg gacgtttttc ggtaccctta aaacacatat gctctaaaaa atgcgaaata
    16741 cccgcttcgt taatagattc atattctgag cccgccttta ccaaaatcaa aaaagtaacg
    16801 ttagtggatt cttttagcgg tattaaaagc aaatttaaac cttttttagt taaaaaagaa
    16861 atttttgttt gcattgtcac attatttttc ttcttcccat ttataagctg catgagctgt
    16921 aattttatat tcaacaattt tacctttttc caaagaaacc gattggctta
//
LOCUS       DAOSMC010000010        16248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_4260_length_16248_cov_18.476379, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000010 DAOSMC010000000
VERSION     DAOSMC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16248
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4260_length_16248_cov_18.476379"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..743
                     /locus_tag="PL075_01635"
     CDS             183..743
                     /locus_tag="PL075_01635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOD96929.1"
                     /translation="MRSLKNLKWIILFIFLLLIFLCAVKFYPATNYTYQIRVDLKKLQ
                     LTLKECNTGREIYCFPIAGPASINYLKPLPKIGEVSKVTFHPYWYPTENIKKKYLKTH
                     GVELPDVVPPNHPFNALGTVAISFVVDGKEQIVKIHGTNDPKSIGHYVSSGCIRMHNE
                     DVEQLAKTICNARTQVIIFYDELKTN"
     gene            982..2871
                     /gene="uvrB"
                     /locus_tag="PL075_01640"
     CDS             982..2871
                     /gene="uvrB"
                     /locus_tag="PL075_01640"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOD96930.1"
                     /translation="MLINNPLSPNDLGVGVLLLIMFKIKAPYKPAGDQPKAIKQLIKG
                     LNKGHHFQTLLGVTGSGKTFTMANVIAAYNKPTLVIAPNKALAAQLYREYKTFFPENE
                     VCYFVSYYDYYQPEAYIPITDTYISKEAIINEEIDRLRHKATSALLKKRDVIVVASVS
                     CIYNLGLPTNYFSSAIHLEVGQPLMRNDLIKQLVKIHFERTNSDLKRGAFRVRGDTFE
                     LIPASGEVINRIELSDQKITRLSIVDATTRDVKKDLPELVILPFKHFISTIPQREKAI
                     QNIKEELKERVEFFRKQKKFLEAERLERRTLNDLMMIKNLGYCHGIENYSRHLLNKLP
                     GEPPETLLAYFPYQNGKPDFLTIIDESHITIPQIRGMYEGDRSRKEILVEYGFRLPSA
                     LDNRPLKFKEFLERTSSIIFTSATPSKFEIENSSQVVEQIIRPTFLVDPPIEIRPVFD
                     KKKNRSEIDDIINEIKITVKNGGRVLINTLTKKTAEELTDYLKNLKIKANYMHSDTKT
                     MERAQILTDFRKGKFDVLIGVNLLREGLDLPEVTIVAVLDADREGFLRSSVSLIQLMG
                     RAARNVKGKIILYADTITDSIKTAIKEANRRRLIQMNYNKKHHLTPQSIQKEIYNLIE
                     KEELK"
     gene            2881..5382
                     /gene="uvrA"
                     /locus_tag="PL075_01645"
     CDS             2881..5382
                     /gene="uvrA"
                     /locus_tag="PL075_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOD96931.1"
                     /translation="MKEEKIKIIGARTHNLKNINLELPKNKLIVFTGLSGSGKSSLAF
                     DTLFAEGQRKYIESLSPYARQFLGQMEKPDVDDIEGLSPAIAINQRALSHNPRSIVAT
                     LTEIYDYLRVLFARIGKPYCPLCGTPIEKLSPEEIFNLIINKAKELKLDYVTILSPVV
                     YGRKGEYYQLLYDYLNKGFSEAVIDGKLHSLHDKVVLSRYKIHNIEIVIDRPLIKDGT
                     RLFEAIEMALDYSNGLIDVSFPTEKEFHILYSNRFSCPKDGFSFPEIEPRLFSFNSPY
                     GACPTCHGLGKEDIFLNTICPDCQGKRLKPETLAVKVSNKNIWEIISQTIEEAYEFFF
                     NLEEKLTEKERRIAHILIEEILSRLGFLLEVGLDYLTLNREAETLSGGEAQRIRLASQ
                     LGSKLSNTLYVLDEPTIGLHERDTEKLLKTLKKLKELNNTVIVVEHDERTIASSDYFV
                     ELGPGAGVNGGEIVVAGETQKVLNDKKIKSLTLKYLRHERQIPHFNRRTNITEKIKII
                     GCRLNNLKNIDVEIPLRKLIAITGVSGSGKSSLLEVIRRSVDHSLGRFTSPLEYASKI
                     FGTEYVRRIVEINQSPIGRTPRSNPATYTGVFNPIRDFFASLEDARSRGYTKSRFSFN
                     VKGGRCEACEGAGYKLIEMHFLPPILVQCEVCRGKRFNRETLEVKYNKKSIADVLELT
                     VDEAYEFFEDIYPIEEKLKILKEMGLGYIRLGQSATTLSGGEAQRIKLAKELSQTATK
                     TIYLLDEPTVGLHYYDIELLLKILEQLVNQGNTVLVVEHNLHFINACDHIIDLGPEGG
                     DKGGKIVATGTPAEIMRNKNSYTGLYLKKLNQLRS"
     gene            5388..6539
                     /locus_tag="PL075_01650"
     CDS             5388..6539
                     /locus_tag="PL075_01650"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOD96932.1"
                     /translation="MNQKIAQKIKKAPLAPGVYFFRNKDSETIYIGRAANLKNRLKNY
                     LAPTDPKTKKMTEEAVSVEWKTTKNLLEAIVLEANLIKKCQPFFNIKEKDNRSFVYII
                     IPQTKWPYPRIVRGRELAKFQPHNTFIFGPLQSYSLAKNFLHSLRKVFPYSTCAFNSG
                     RPCFHYQIGLCLGKCVNKISETDYNKIIQAMINFLQGKIKKAKIFLKKNYPEKLKLFD
                     QMEDAYLITNEEFRLSPQLKIEGYDISHFGGKETVGAMVVFQNQDIDPSQYRLFKIRN
                     APKNDDLLALAEMFERRLNHPEWPYPNLILVDGGREQVKIFEKVLAQRKISIPVVGIS
                     KFQNDKLIFGKMKKSVKDLVELSKNDLLKLRDEAHRFANNYRKKLMSVK"
     gene            6766..7164
                     /locus_tag="PL075_01655"
     CDS             6766..7164
                     /locus_tag="PL075_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOD96933.1"
                     /translation="MKGFFGHIEKDTLENENFRKVLYTGKHCQLVLMSLKPNEDIGME
                     VHPDNDQFFRFETGTGKVIIDGNEYNVTDGDAIIVPAGAEHNVINTSDSEPLKLYTIY
                     APPHHQDGVVRATKEEAELDSPEFDGKTTE"
     gene            complement(7217..7396)
                     /locus_tag="PL075_01660"
     CDS             complement(7217..7396)
                     /locus_tag="PL075_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96934.1"
                     /translation="MLAEVLFVSIEIKFSHFWYWVIAGIFQLGWLLVMGLCRVAGESD
                     ERMEKIMEEELKKKN"
     gene            complement(7576..7719)
                     /locus_tag="PL075_01665"
     CDS             complement(7576..7719)
                     /locus_tag="PL075_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96935.1"
                     /translation="MTENKNAQNDAPPLRHPEDFSPKDLMAFSNNQQSITLHGILRAS
                     PSE"
     gene            complement(7972..9561)
                     /locus_tag="PL075_01670"
     CDS             complement(7972..9561)
                     /locus_tag="PL075_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOD96936.1"
                     /translation="MKKLDEIIEQLIKKTTLSRQQLEAIKRRYAEENKVDLPTNIELL
                     KVYHNLVQKKRIYPIAKVESLLKTRPMRSLSGIVNITVLTKPFGCPGRCIFCPNEMGF
                     PKSYLKGEPAADRAYDLQFDPYLQVQKRLEMLDDQGHSIDKVELRIVGGSWSFYPKSY
                     QNWFVKECYRAADDFQRKRKYPSRLSLEKLQKINETARVRIVGMSVETRPDLITPSEL
                     RNLRRLGVTMVEIGVQTIFDDVHQLNKTNLNAAIIERATRLLKNAGFKVLYHLMPNLY
                     GSDLLKDKEMVRIVFNDSRFKPDWLKIYPTVVLENTPLYQLYQAGKYRPYTDEELFDL
                     LIEIKTKLPYWVRVARIIRDIPAQKVVGGTHLSNLREIIQKRMKEKGLICHCIRCREV
                     KENYNPKEKIYLFREDYEASDGWEVFLSFENKERSKLFSLLRLRCPSFEEKPIFKNLE
                     GAVLIREIKTFGELVPLSEKKIAPQHKGLGKRLMKEAERIAVEEFNLHKIAVIAGVGV
                     RGYFRRLGYRLNKSYMVKNVK"
     gene            9560..10297
                     /locus_tag="PL075_01675"
     CDS             9560..10297
                     /locus_tag="PL075_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOD96937.1"
                     /translation="MKKDFAHYLLEKTQRDYNLIAEDFSRTRQNIPPDFKQFLQPYFS
                     FDSQIKILDWGCGNGRFYSLFKDCLYYGVDISENLINLAKQEYPSAKFFLLHSPLSLP
                     FDNNFFDEIICLAVFHHIPSQEFRQQFLQEAWRVLKPNGIMLLTVWDLRIGWLLRSGN
                     WKRLFLRIKYFFLHLFGRTPLNGGDFYIPWAHHCNRYLHAFSFKELNKLFQTTGFQIL
                     QSGTRKDFIHSKERNIYFFVRKTKFPQ"
     gene            complement(10281..11993)
                     /locus_tag="PL075_01680"
     CDS             complement(10281..11993)
                     /locus_tag="PL075_01680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOD96938.1"
                     /translation="MFQQNKTAKNFKQSQDKIKICVLGGVGEVGRNLSFIEYRNQILV
                     FDCGLRFPEEDMPGIDFIIPNVDYLEKNRKKILGMFITHAHYDHIGAIPYIVEKLGNP
                     TIYTAPLTRAIILKRQEDFPHQKKMDIETIDMATLKPISVGNFRVHPYLVNHNVPDTL
                     GFAVETFLGTIFYMPDFKVDFSPIIDPPIDLGQVAFLAAKNPLVLMMDSTGAEEAGHS
                     ISERGIYEDLERIFERASGRIIVATFASLISRLQQIIWLSEKFNRRVVIDGYSMKSNV
                     EVARMMGYLKINKNTLISPQEAQGLLSRQLTILCTGSQGEDRAVLMRIANREHKYFRV
                     EKGDTVIFSSSVVPGNERTVQNLKDTFYRQGARVFHYKMMDIHAGGHAQQEELKLMLN
                     LIRPKFLVPIHGQYSMLQAQADIAEQLGMKRENIVIANNGQIIEVTKNAIAATNQEVP
                     ANYVMVDGLGVGDVGEVVLRDRHQLSKDGMLVIVTVVDGESGELRGDPEISSRGFVYL
                     KESQKLMNETKQLVKDIIQKTASKDRTTNWAYVKDNLRDKVGEFLFKETQRRPMILPF
                     IVEI"
     gene            complement(12221..12304)
                     /locus_tag="PL075_01685"
     tRNA            complement(12221..12304)
                     /locus_tag="PL075_01685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12267..12269),aa:Leu,seq:cag)
     gene            complement(12411..14132)
                     /locus_tag="PL075_01690"
     CDS             complement(12411..14132)
                     /locus_tag="PL075_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOD96939.1"
                     /translation="MKVKYFFQKSFWQNFWHSLSDVEKKIFWGALILMVGSLIIGGVF
                     WYLNHTTLVAKEGGVLIEGMVGQPTTINPLFAANEIDQSLSSLVYRGLLKSDGKGGII
                     NDLADSYEKSEDGRHWVVHLKKDVFWHDGTPFTADDVVFTINAIQNPDTRSPYRTAWQ
                     GVTVTKISDYTLTFDLKNPYAFFEENLKQKIIPKHIFGEIPLANIRDLSAYNFEPIGT
                     GPFIYEKFDATKKGYINYYIFNANERFYNGRPFIDSYIIKFYQSETEALKAFEYHQID
                     LLSSVSTLNLKNINRSHQLYEYSLPQYFAVFLNAIISKPLSDRNVRYALNYATNRKEI
                     IDEVFQGRAEAAEGPILSWMPGYNPNLRAEYSLDKAKQILVTAGWEDADGDGTVEKIL
                     NKNEDPTPLKITLITPSTDFLIQTASLLKKQWKEIGAEVEIKIVEFSDLQNNYLTTRL
                     YDALLYGNIINQKPDLFSFWHSSQKFYPGLNLSLYENASVDRFLEDARLTASDDEYKK
                     DLANIQSSILTDMPAIFLYNPKVLLVALPTLHIENLEKLNISSERYDNVYEWYLKTER
                     ILVDSND"
     gene            complement(14140..14361)
                     /gene="secG"
                     /locus_tag="PL075_01695"
     CDS             complement(14140..14361)
                     /gene="secG"
                     /locus_tag="PL075_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOD96940.1"
                     /translation="MNSNLIAIIQIVVSIALIVLILLQPRGMGLGSIFGGGAEFYQRR
                     RGIEKATFYITIVLAGIFLVLGVVALVAK"
     gene            complement(14363..14716)
                     /locus_tag="PL075_01700"
     CDS             complement(14363..14716)
                     /locus_tag="PL075_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOD96941.1"
                     /translation="MKFLSRLVLQVVLNAVGLWFIIQTVPNISYGGDLARLIATALVL
                     GILNFVLKPILKLVLSPLIALTLGLLTFVINAFILYLATQLVPSLVIPLGWPLIWATL
                     IMSIINFLFNLLAKK"
     gene            complement(14765..15361)
                     /locus_tag="PL075_01705"
     CDS             complement(14765..15361)
                     /locus_tag="PL075_01705"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOD96942.1"
                     /translation="MTKKDIIFSAIIGLFIAFLIWPLWLNLGVLLNYWSYRWLLVIVC
                     PFISMLVVVIAFWLKKLNDVIWQFAKYALVGVLNTLLDFGILNLLSYLTKIYQGGWLI
                     FFNFFAFFIANINSYFWNKYWSFDKGGKAKTQEFTKFFVVSLIGFGLNSFVLWGITSA
                     SPQMNLTPSQWENIGKLGGTLISLIWNFVGYKIFVFKK"
     gene            complement(15369..15440)
                     /locus_tag="PL075_01710"
     tRNA            complement(15369..15440)
                     /locus_tag="PL075_01710"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15405..15407),aa:Trp,seq:cca)
     gene            complement(15463..15621)
                     /gene="rpmG"
                     /locus_tag="PL075_01715"
     CDS             complement(15463..15621)
                     /gene="rpmG"
                     /locus_tag="PL075_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOD96943.1"
                     /translation="MPQENLIKLQCAVCKKINYYTRKNKKTIQKKIELKKFCPFCKKR
                     TIHKEIKK"
     gene            complement(15854..15925)
                     /locus_tag="PL075_01720"
     tRNA            complement(15854..15925)
                     /locus_tag="PL075_01720"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15891..15893),aa:Thr,seq:ggt)
     gene            complement(16043..16126)
                     /locus_tag="PL075_01725"
     tRNA            complement(16043..16126)
                     /locus_tag="PL075_01725"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16090..16092),aa:Tyr,seq:gta)
     gene            complement(16170..16242)
                     /locus_tag="PL075_01730"
     tRNA            complement(16170..16242)
                     /locus_tag="PL075_01730"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16207..16209),aa:Thr,seq:tgt)
BASE COUNT         5356 a         3135 c         2595 g         5162 t
ORIGIN      
        1 agcataacag cgccgcctgc ctcttccgtc cctaatttat ggtgttatgc tttagcataa
       61 cagcgccgcc tgcttcttcc attcctaact tctgctgtta tgctaaagca taacagcaga
      121 aattcttaaa gggctcttgt aaaaaattgt caaattatta agatagaata aggaggtaaa
      181 aaatgcgttc tttaaaaaat ctaaaatgga ttattttatt tatttttctg cttttaattt
      241 ttctttgtgc tgtcaagttt tatcctgcaa caaactacac ttatcaaata cgtgttgatt
      301 taaagaaatt gcagctcacg cttaaagaat gtaatacggg aagagaaatt tattgcttcc
      361 caattgccgg tcctgcttca atcaattatc tgaaaccatt gccaaaaatt ggggaagttt
      421 ctaaggttac atttcaccca tattggtatc ccacagaaaa tatcaaaaag aagtatttaa
      481 aaacccacgg tgtagaattg cccgatgttg taccgcccaa tcaccctttt aatgctttgg
      541 gaactgttgc tatttctttt gttgtagacg gcaaagagca gattgttaaa attcatggga
      601 ccaacgatcc aaaatctatt ggacactatg tcagtagcgg ctgtatcaga atgcacaatg
      661 aagatgttga acagcttgcg aaaactatct gcaatgccag aacccaagtg attatttttt
      721 atgacgaatt aaaaacgaat taaaatgaaa aaaattccgg cccaaaaacc ggaattttta
      781 tttatcatta atcttatttt gataataaat tttcagttta attgagggcg gcggaaattt
      841 tctttaagaa aatttccgcc gccacattat aattgcctcg ccactttcat ctcttttaat
      901 attttatttc ttcgcatctt ccattataca tttaggctta tgtaaaaatt tgctttttgc
      961 tttttttatg ttataatttt aatgctaatc aacaaccccc tatcccctaa tgatctcgga
     1021 gtgggggtgc ttttacttat tatgtttaaa attaaagcgc catacaaacc agcgggtgac
     1081 caacccaaag caattaaaca attaatcaaa ggcctcaaca aaggtcatca ttttcaaact
     1141 cttttaggtg taactggcag cggcaaaact tttacaatgg ccaatgtgat tgctgcctat
     1201 aacaaaccaa cattagttat tgctcccaac aaagccttag ccgctcaact gtatcgggaa
     1261 tataaaactt tttttcctga aaatgaagtt tgttatttcg tttcttatta tgactattat
     1321 caacccgaag cctatattcc tattaccgat acatatattt ccaaagaagc tattattaat
     1381 gaagagatcg atcgccttcg ccataaagcc acttccgctt tgcttaaaaa aagagatgtt
     1441 attgtggtcg cttcggtttc ttgcatttat aatcttggct tgccaactaa ttatttctca
     1501 tcagctattc atttagaagt aggtcaaccc ctaatgcgca atgatcttat taaacaatta
     1561 gtgaaaattc attttgaacg aacaaatagc gatttaaaac gcggggcttt ccgcgttcgc
     1621 ggtgacacat ttgaattaat tcccgcttcc ggagaagtca ttaatcgcat tgagttatct
     1681 gatcaaaaaa ttactcgtct ttctattgta gatgcaacta caagagatgt aaaaaaagat
     1741 cttccagaat tagtcatttt gccttttaag cattttattt ccacgattcc ccaacgcgaa
     1801 aaagccattc aaaacattaa agaagaattg aaagaacgtg tagaattttt ccgtaaacag
     1861 aaaaagtttt tagaagccga gcgattggaa cgtcgaacac tcaatgactt aatgatgatt
     1921 aaaaatttag gttattgtca tggaattgaa aattattctc gtcatctttt aaacaaacta
     1981 ccaggtgaac cgcctgaaac tttattagct tatttcccct accaaaatgg caaaccagat
     2041 tttttaacca ttattgatga atcgcatatc actatcccgc aaattcgagg catgtatgag
     2101 ggtgatcgca gtcgtaaaga aatactggtt gaatatggtt ttcgcttacc ttcggctttg
     2161 gataatcgtc ctttaaaatt taaagagttt ttggaacgca ctagctctat catttttact
     2221 tctgctactc catcaaaatt tgaaatagag aattctagcc aagtggttga acaaattatt
     2281 cgtcccacat ttttagttga tccacccatt gaaatccgac ctgtttttga taaaaagaaa
     2341 aaccgtagcg agattgacga tatcattaat gaaataaaaa taacggtgaa aaatggaggc
     2401 cgcgttttaa tcaataccct aacaaaaaaa actgctgaag aattaactga ttatcttaaa
     2461 aatttaaaaa tcaaagccaa ttatatgcac tctgacacaa aaactatgga acgagctcaa
     2521 attctaactg attttcggaa aggtaaattt gatgttctaa tcggcgtcaa tcttttgcgc
     2581 gaaggattgg atttacccga agtaaccatt gtggctgttc ttgatgctga tcgagaagga
     2641 tttttacgca gcagcgtttc gcttattcaa ttaatgggcc gcgccgcgcg caatgttaaa
     2701 ggaaaaatta ttctttatgc tgacaccatt actgattcaa taaaaacagc tataaaagag
     2761 gctaaccgcc gccgtctgat ccaaatgaat tataataaaa aacatcatct tactccccaa
     2821 agtattcaaa aagaaattta caatcttatt gaaaaagaag aattaaaata aattaaaaat
     2881 atgaaagaag aaaaaataaa aattattggc gctcgcactc acaacttaaa aaatatcaat
     2941 ctcgaactgc caaaaaataa attaattgtt ttcactggat tatcgggttc gggaaaatct
     3001 tctttggctt ttgatacttt atttgctgaa ggccaaagaa aatatatcga atctctttcg
     3061 ccctatgcta gacagttttt aggtcaaatg gaaaaacccg acgttgatga tattgaagga
     3121 ctttcaccag caatcgccat taaccagcgc gctctttctc ataatcctcg ctccattgtg
     3181 gccacactta cggaaattta tgattatctt cgtgtgcttt ttgctcgcat tggtaaacct
     3241 tattgccctc tttgcggcac tcctattgaa aaactttcgc ctgaagaaat ttttaatctt
     3301 attattaaca aagcaaaaga gctgaaactt gactatgtga caattttgtc accagttgtt
     3361 tatggtcgaa agggcgaata ttatcagctc ctttatgatt atttaaataa agggttttcc
     3421 gaagccgtta ttgatggtaa gctccactcc ctccatgata aagtggtgtt gtcacgctat
     3481 aaaattcaca atattgaaat tgttattgat cgaccgttaa ttaaagacgg cactcgtctt
     3541 tttgaagcta ttgaaatggc cttggattat agcaacggat taattgatgt ctctttccca
     3601 accgaaaaag aattccacat tctttattct aatcgtttta gttgtcccaa agatgggttt
     3661 tctttcccag aaattgaacc acgtcttttt tcttttaata gcccctatgg cgcctgtcct
     3721 acttgtcatg gtttaggcaa agaagatata ttcctcaata ccatttgtcc tgattgccaa
     3781 ggcaaaagat taaaacccga aacactcgct gtaaaagtat ctaataaaaa tatttgggaa
     3841 attattagcc aaaccattga agaggcttat gagtttttct ttaatcttga agaaaagctt
     3901 actgaaaaag aaagaaggat tgctcatatt ttaattgaag aaattctgag ccgcttaggc
     3961 tttcttctag aggtgggact cgattattta actttaaatc gtgaagcgga aactttgtcc
     4021 ggcggcgaag ctcaacgcat tcgcctcgcc tctcaattag gatctaaact ctctaacact
     4081 ttatatgttt tggatgaacc cacgattggc ttgcacgaac gcgacacaga aaagcttttg
     4141 aaaactttga aaaaattaaa agaattaaac aatacggtaa ttgtggttga gcacgatgaa
     4201 agaacaattg cttcgtcgga ttatttcgta gaactgggac ccggcgcggg tgttaatggc
     4261 ggtgaaatcg tagtagccgg cgaaacgcaa aaagtattaa atgataaaaa aattaagagt
     4321 ttaaccctaa aatatttgcg ccatgaacga caaattcctc attttaatcg tcgtactaat
     4381 attactgaaa aaattaaaat tatcggctgt cgattaaaca acttaaaaaa tattgatgta
     4441 gaaattccct tgcgaaaact catcgctatc actggtgttt caggaagcgg caaatcatcg
     4501 cttttagaag tcatcagaag aagtgtcgat catagtttgg gtcgatttac ttcacctttg
     4561 gaatatgctt ctaaaatttt tgggactgaa tatgtaagac gcattgtaga aattaaccaa
     4621 agcccaattg gccgcacgcc acgctctaat ccagccactt acactggcgt ttttaacccc
     4681 atcagagatt tttttgcttc acttgaggac gcccgcagtc gtggctacac caaaagtcgc
     4741 ttttctttta atgtaaaagg tggcagatgc gaagcctgcg aaggtgctgg ttataaatta
     4801 attgaaatgc actttctacc tccaatttta gtccaatgcg aagtttgccg aggcaaaaga
     4861 tttaatcgtg aaacgcttga agtaaaatac aataagaaaa gcattgctga tgttttagaa
     4921 ttaaccgttg atgaggctta tgaatttttt gaggatattt atcccattga agaaaaatta
     4981 aaaattttaa aagagatggg cttgggctat attagactgg gtcaaagtgc cacaactctt
     5041 tcgggcggtg aagcacaaag gataaaatta gccaaagaac tttctcaaac agctactaaa
     5101 accatctatt tattagacga acccacagtt ggcttacatt attatgatat tgaattgctg
     5161 ctgaaaattc ttgaacaact agtaaatcag ggcaacaccg ttttggtggt tgaacacaat
     5221 ctacatttta ttaatgcatg tgatcacatt attgacttag gcccagaagg tggcgataaa
     5281 ggaggaaaaa tcgtcgcaac gggtacgcct gcagaaatta tgcgaaacaa aaattcttat
     5341 actggtcttt atcttaagaa attaaatcaa cttagaagtt aattatcatg aaccaaaaaa
     5401 tcgctcaaaa aattaaaaaa gctcctcttg ctcctggtgt ctattttttt cgaaataaag
     5461 atagcgaaac tatttatatc ggtcgagctg ccaatttaaa aaatcgttta aaaaattacc
     5521 ttgcgcccac tgatcctaaa accaaaaaaa tgacagaaga agcagttagt gttgaatgga
     5581 aaaccaccaa aaatctttta gaagcaattg ttcttgaagc aaatttaatt aaaaaatgcc
     5641 agcccttttt taatattaaa gaaaaagata accgttcctt tgtttatatt attattcccc
     5701 aaacaaaatg gccctatcct cgtattgttc gcggtcgtga gcttgctaaa ttccaacccc
     5761 acaatacttt tatctttgga ccattacaaa gctattcctt ggcaaaaaat tttcttcatt
     5821 ctctcagaaa agtttttcct tacagtactt gcgcttttaa tagcggccgt ccgtgttttc
     5881 attatcaaat cggtctttgt ctcggtaagt gtgtaaataa aatcagcgaa acagattata
     5941 acaaaattat ccaagcaatg atcaattttc tccaaggcaa aataaaaaaa gcaaaaatat
     6001 tcttaaaaaa gaattatcct gaaaaattaa aattatttga tcaaatggaa gatgcttact
     6061 taattacgaa tgaagaattt cgattatcgc ctcagttaaa aattgagggt tatgacattt
     6121 cgcattttgg cggcaaagaa actgttggag caatggttgt ttttcaaaac caagacattg
     6181 atccgtcgca atatcgcctt ttcaaaatta gaaacgctcc aaaaaatgac gatctgctcg
     6241 ccttggctga aatgtttgaa agaagattaa atcatccaga atggccatat ccaaatctca
     6301 ttcttgtaga tggcggtcgt gaacaggtca aaatatttga aaaagttctc gcacaaagaa
     6361 aaatttctat tccggtagtg ggtatttcca aatttcagaa tgataaattg attttcggaa
     6421 aaatgaaaaa atctgttaaa gatttagttg aactttctaa aaatgacctg ctaaaactcc
     6481 gcgatgaagc tcatcgtttt gctaacaatt atcgcaaaaa actgatgagt gttaaatgaa
     6541 ggccggggtt ttatgaaatt gaacaggcgg cggaaaattt tctttcagaa aattttccgc
     6601 cgtatttata ttatttttag ctcatgaatt aattcatgaa ttaattcatg agctctttga
     6661 aatatccttt taaaatactt ctaaactata ttaaaaattg actaactgtt ttattttttg
     6721 ctatactgtt aaagtaatca aaaagtcgct taaataaata taaatatgaa agggtttttt
     6781 ggacatattg aaaaagacac tttagaaaat gaaaattttc gaaaggtttt atataccggt
     6841 aaacattgcc aattggtctt aatgagtctt aaacctaatg aagacattgg catggaagtt
     6901 catccggata atgatcagtt tttccgtttt gaaacaggaa caggaaaagt gatcattgac
     6961 ggcaatgaat ataatgttac agacggagat gctattattg ttcccgcggg cgcagaacac
     7021 aatgttatta acacctcgga cagcgaaccg ttaaagctct acaccattta cgctcctcct
     7081 catcatcaag atggtgttgt acgcgccaca aaagaagaag cagaattgga ttcaccagaa
     7141 tttgacggca aaacaaccga ataatattac aaaatgtatg atgaaaaagt cgcccgcggg
     7201 cgactttttt aattcattaa tttttctttt ttaattcttc ttccataatc ttttccattc
     7261 tttcatcgct ttcaccagct acccgacata gtcccattac caaaagccat cctaattgaa
     7321 aaattccagc aataacccag taccaaaaat gactaaattt aatttcaata gaaacaaaca
     7381 atacttctgc taacataatc ggtcagagac agagaattaa ataagcaacg aagtttgcta
     7441 tttttttgtt aagtttcatt tttcacctcc tccaatcatt ataaaattgc aaatttaaaa
     7501 tgcaagctga gaaattcttc gggttgtatt tgcccacctt tggtgggcat ttaaaatgac
     7561 agtggaaaaa aggtgtcatt ctgagggcga agcccgaaga atcccatgca atgtaattga
     7621 ttgctgattg ttgctgaaag ccatgagatc cttcggacta aagtcctcag gatgacgaag
     7681 tggtggtgcg tcattctgag cgtttttatt ttctgtcatt ctgaacattt ttttactgtc
     7741 attctgagcg ccagcgaaga atcccatcca cagcagtaat agttgcacaa tgctaaaatc
     7801 catggaatcc ttcactaaag ttcaggatga cgaagaaaaa caggataaca ataaatgtag
     7861 gatagtaaag cggtggaaaa tttcaaagaa attttccacc gctgctatta aattagctca
     7921 tgaattaatt catgagctaa ttgattttat ccgcctatta acagcagagc ttcattttac
     7981 attcttaacc atatatgatt tattcaaccg atatcctaat cttctaaaat atcctcgcac
     8041 gcccacacca gcaataactg caattttgtg taagttaaat tcttctacgg caattcgttc
     8101 cgcttctttc attaatcgtt tccccaaccc tttatgttga ggggctatct ttttttctga
     8161 caaaggaaca agttcgccaa aagttttaat ttctcgaatc aaaaccgctc cctctaaatt
     8221 cttaaatatg ggcttctctt caaacgaagg gcatcgtaaa cggagcaaac taaaaagttt
     8281 gctgcgttct ttattttcaa aacttaaaaa gacttcccaa ccatctgacg cctcgtaatc
     8341 ttctcgaaat aaataaattt tctctttagg attataattt tcctttacct cccggcatct
     8401 aatacaatgg catatcagcc ccttttcttt cattcttttt tgaataattt cgcgaagatt
     8461 agacaggtga gtgcctccca ccactttttg agccgggata tctcgaataa tccgcgccac
     8521 tcttacccaa taaggcaatt tcgttttaat ttcgattaaa agatcaaaaa gttcttcatc
     8581 ggtataaggc cgatattttc ctgcttggta taattgataa agaggtgtat tttccaaaac
     8641 aacagtagga taaatcttta accaatctgg tttaaaacgc gaatcattaa aaacaatgcg
     8701 caccatttct ttgtctttta gcaaatcaga tccataaaga ttaggcatta aatggtaaag
     8761 aactttaaaa ccagcatttt taagcaatct tgtcgctcgc tcaataatcg ccgcatttaa
     8821 attagtttta ttcagttgat gaacgtcatc aaaaatagtt tgaacaccaa tttcaaccat
     8881 tgtaaccccc aaacgcctta aattccttaa ctcactaggc gtaattaaat caggccgcgt
     8941 ttctactgac atacccacta ttcttactcg ggctgtttca tttatttttt gtaatttttc
     9001 aagcgataaa cgactcggat attttctttt tctttgaaaa tcatccgccg cgcgatagca
     9061 ctccttcaca aaccaattct gataactttt tggataaaaa gaccagctcc caccaacaat
     9121 acgcaattcc accttatcaa tagaatgtcc ctgatcatca agcatctcta atcttttttg
     9181 aacttgcaaa taaggatcaa attgtaaatc ataagctcga tcagcagctg gttcgccttt
     9241 taaataactc ttgggaaatc ccatttcatt cggacaaaaa atacagcggc cgggacaacc
     9301 aaaaggcttc gtcaaaactg taatattaac aatgcctgat aaagacctca ttggccgcgt
     9361 ttttaataag ctttctactt ttgcaatagg ataaattctt ttcttttgaa cgaggttatg
     9421 ataaactttt aatagttcta tattggttgg caaatcaact ttattttctt ctgcataccg
     9481 ccttttgatt gcttctagtt gctgccgact taaagtagtt tttttaatta attgttcaat
     9541 tatttcatct aattttttca tgaaaaaaga ttttgcccat tatctattag aaaaaactca
     9601 aagagattat aacttaattg ctgaagattt ttcaagaaca cgccaaaata ttcctcccga
     9661 cttcaaacaa tttcttcaac catatttttc ttttgattct caaatcaaaa ttttagattg
     9721 gggttgcggc aatggccgtt tttactccct cttcaaagat tgtctttatt atggcgtgga
     9781 catttcagaa aaccttatta atctcgccaa acaagaatat ccttcagcaa agttttttct
     9841 tcttcattcg cctctctcgc ttccttttga taataatttc tttgatgaaa taatttgtct
     9901 cgctgttttt catcacattc cttctcaaga atttcgccag caatttttgc aagaagcttg
     9961 gcgtgtttta aaaccaaatg gcataatgct gctgactgtt tgggatttga gaattggctg
    10021 gctcctccgc agtggtaatt ggaaacgtct ctttttaaga ataaagtact tctttcttca
    10081 tttattcgga cgcactccat taaatggagg agatttctat attccgtggg ctcatcactg
    10141 caatcgttat cttcacgcgt tttctttcaa agaattaaac aaactgtttc aaacgacagg
    10201 gtttcaaatt cttcagtcag gcacgcgcaa agacttcatt cattcaaaag aaagaaacat
    10261 ctattttttt gttcgaaaaa ctaaatttcc acaataaatg gcaagatcat tggccgtctt
    10321 tgagtttctt taaaaagaaa ttctcctact ttatccctca aattatcttt tacatatgcc
    10381 caattcgttg tgcgatcttt tgaagccgtt ttttgaataa tatctttgac gagttgtttg
    10441 gtttcattca ttaatttttg cgattctttt agataaacaa aaccgcgtga agaaatttct
    10501 ggatcgcctc gcaattcacc actttcgcca tcaaccacag taacaatcac caacatacca
    10561 tcttttgaaa gctggtgacg atctcttagc actacctcgc caacatcgcc cactcccaaa
    10621 ccatcaacca ttacataatt cgctggcacc tcttgattag tagctgcaat tgcatttttc
    10681 gttacttcaa taatttgacc gttattcgct ataacgatat tttcgcgttt cattcccaat
    10741 tgttctgcta tgtctgcttg cgcttgcaac atagaatatt gtccgtgaat aggcactaaa
    10801 aatttaggcc gaatcaaatt gagcattagt tttaactctt cttgttgagc atggccacca
    10861 gcatgaatgt ccatcatttt ataatgaaat actctggctc cctgtcgata aaaagtatct
    10921 tttaaattct gtactgttct ttcgttgccc gggacaacgg aagaagaaaa aatcaccgtg
    10981 tcgccttttt ctactctaaa atatttatgc tcacgattag cgatgcgcat taaaacagcc
    11041 ctatcctctc cttgcgatcc agtacacaaa atcgtcaact gacgggaaag aagtccttgc
    11101 gcttcttgtg gcgaaataag cgtgtttttg tttattttta aataccccat cattcgagcc
    11161 acttctacat tacttttcat actataaccg tcaatgacta ctcgacgatt aaatttttct
    11221 gaaagccaga taatttgctg caaacgacta atcaatgagg caaaagtagc aacaataatt
    11281 cgtccagaag ctcgttcaaa aatacgctct aaatcttcat aaatacctct ttcggaaata
    11341 gaatgacctg cttcttccgc tcccgttgaa tccatcatta aaactaatgg atttttagcg
    11401 gctaaaaaag caacctggcc taaatcaata ggcggatcaa taataggact aaaatcaacc
    11461 ttaaaatctg gcatataaaa aattgttccc aaaaaagttt ctacggcaaa tcctaatgtg
    11521 tctggaacgt tgtgattaac caaataggga tgcactcgaa aattaccaac agaaattggc
    11581 ttcaaagtgg ccatatcaat cgtttcaata tccatttttt tttgatgagg aaaatcttct
    11641 tgccttttaa gaataatagc tcgcgtcaaa ggcgctgtat aaatcgttgg attacctaac
    11701 ttttcaacga tatagggaat agcgccgatg tgatcatagt gagcatgcgt aataaacatt
    11761 cccaaaatct ttttccgatt tttttctaaa taatcaacgt tgggaataat aaaatcgatg
    11821 cccggcatgt cttcttcagg aaaacgcaat ccacaatcaa aaaccaaaat ttgatttcta
    11881 tactcaataa aggacagatt gcgaccgacc tcacctacac cgccgaggac acaaattttt
    11941 attttatcct gcgactgttt aaaatttttc gctgttttat tttgttgaaa catatttttc
    12001 ttatttttcc cgctttgccc gattttaagc gaacaaagaa gttaaattat aaaagttgat
    12061 taaataaatt cagaaattaa aagaccaatt atgcaacaag taaaaaatca aaaataaatt
    12121 gtaacgcttt gcggttaaaa accgcctatc gactttaaaa tttttaatta ttaattttga
    12181 ttctctatct tttgattctg aattttaatc ttacttgtag tgccgcgggt gggatttgaa
    12241 cccacatccc gaaacgggac acggccctga accgtgcgcg tctgccaaat tccgccaccg
    12301 cggcaaaaaa taatcttatt tagtaaaaag ctgctttagt ttaattaaaa tttttattgg
    12361 atactcgtta atttatcact gttttaatat tttatttccc caatttatta ttaatcgttt
    12421 gaatcaacta aaatgcgctc cgtctttaaa taccactcat aaacattatc atatcgctct
    12481 gacgaaatat ttaatttttc taaattttca atatgtaatg tcggtagcgc aacaagaaga
    12541 accttaggat tgtaaagaaa aatagctggc atatcagtta aaattgatga ctgaatatta
    12601 gctaaatcct ttttatactc atcatcactt gccgttaaac gcgcatcttc taaaaatcta
    12661 tcaacggaag cattttcata taaagataaa tttaatcccg gatagaattt ttgagaagaa
    12721 tgccaaaaag aaaaaaggtc tggtttttga ttaatgatat tgccataaag taaggcatca
    12781 taaagccgtg tagttaaata gttattttgt aaatctgaaa attcaacaat tttaatttcc
    12841 acctccgctc caatttcctt ccattgtttt tttaataaac tagctgtttg aattaaaaag
    12901 tctgtggaag gagtaataag agtaatcttt aaaggagtgg ggtcttcatt tttgtttaaa
    12961 atcttttcca ccgtgccatc accatctgca tcttcccaac ctgcagtcac taaaatttgc
    13021 ttagctttat ctagtgaata ttccgctctt aaattcggat tatatcccgg catccatgaa
    13081 agaataggcc cctctgctgc ttcagctctc ccttgaaaaa cttcgtcaat aatttcttta
    13141 cgattggtag cgtaatttaa agcataacgc acattacgat ctgacaatgg cttagaaatg
    13201 attgcattaa gaaaaacagc aaaatattga ggcaaggaat attcataaag ctgatgagag
    13261 cgattaatgt ttttgagatt aagagtagaa acactactca ataaatcaat ttgatgatat
    13321 tcaaaggctt tcaaggcttc ggtttctgat tgataaaatt taataatata actatcaatg
    13381 aatggtcggc cattataaaa acgttcatta gcattaaaga tgtaataatt tatatatcct
    13441 tttttggtag catcaaattt ttcataaata aaaggacccg tgccgattgg ttcaaaatta
    13501 taagcagaaa gatcgcgtat gttggctaaa ggaatctctc caaaaatatg ctttggaata
    13561 attttttgtt ttaaattttc ttcaaaaaaa gcataggggt tttttaaatc aaacgtaagt
    13621 gtataatcac taattttggt cactgtcact ccttgccacg ctgttcgata agggcttctg
    13681 gtatcgggat tctgaatagc gttaattgta aaaaccacat cgtctgctgt aaaaggagta
    13741 ccatcgtgcc aaaaaacatc ttttttcaaa tgaacaaccc agtgacgccc gtcttcgctc
    13801 ttttcgtaag aatctgctaa atcattaata ataccgccct tgccgtcgct ttttaataat
    13861 cctcgataaa cgagtgaaga caaactttga tctatttcgt ttgctgcaaa aagcggatta
    13921 atagtcgttg gttgccccac catgccttct attaaaacgc cgccttcttt cgctactaat
    13981 gtcgtatggt ttaaatacca gaaaacacct ccgatgatca acgatccgac catcaaaatt
    14041 aaggcgcccc agaaaatctt cttttcaaca tcggaaagtg aatgccaaaa attctgccaa
    14101 aaactttttt gaaaaaaata ttttactttc atatctcaac tattttgcca ccaaagccac
    14161 cacacccaat accaaaaaaa ttccagcgag aacaattgta atataaaaag ttgccttttc
    14221 tattcctcga cgacgttgat aaaattccgc accacctccg aaaatgcttc ctaatcccat
    14281 ccctcttggt tgaagaagaa taagaacaat taaagcaatg gaaacgacaa tttgaataat
    14341 ggcaattaaa ttactattca ttttattttt ttgctaaaag attaaataga aaattaatga
    14401 tcgacataat aagagtagcc cagatcaacg gccaacccaa cggaattact aaactaggca
    14461 ctaattgggt cgctaaatat aaaataaaag cattgataac aaaagttaaa agtccaaggg
    14521 ttaaggcaat taaagggcta agcactaact tgagaatggg ttttaaaacg aagtttaaaa
    14581 tccccagaac taaagcggtg gctatcaatc gtgccaaatc gccaccataa gaaatattag
    14641 gcaccgtttg aataataaac cacagtccca ctgcgttcaa taccacctga agtactaatc
    14701 gcgataaaaa tttcatattt tatatcttac ttagtatttt aattagatat tttcaaaaat
    14761 caaatcattt cttaaaaaca aatattttat aacctacaaa attccaaata agactaatga
    14821 gtgttcctcc taattttcca atgttttccc attgagatgg cgttaagttc atctgcggcg
    14881 aagcggaagt aatgccccat aaaacaaaag aatttaaacc gaatccaatt aaacttacta
    14941 caaaaaattt agtaaactct tgtgtttttg ccttaccccc cttatcaaaa ctccagtatt
    15001 tattccaaaa ataactattg atattagcaa taaaaaaagc aaaaaaatta aaaaagatca
    15061 accacccgcc ttgataaatt ttagtcagat agcttaataa attcaaaata ccaaaatcaa
    15121 gaagtgtatt aagaacgcca actagcgcat atttagcaaa ttgccaaata acatcattaa
    15181 gcttttttaa ccaaaaagcg atcaccacca ccaacatgct aataaacgga caaacaataa
    15241 ctagcagcca acgatacgac cagtaattta gcaaaactcc taaattaagc cagagcggcc
    15301 aaatcaaaaa agcaatgaat agtccgataa tagcactaaa tatgatgtct tttttagtca
    15361 tattttagcg ggcccggcag gactcgaacc tgcagtcctg gttttggaga ccagtggttt
    15421 gccaattaac cgacggaccc cacattttat tagaatggtc ttctattttt taatttcttt
    15481 gtgaattgtg cgttttttac aaaaaggaca gaattttttc aattctattt ttttctgtat
    15541 tgttttttta tttttacgag tgtaataatt gattttttta caaactgcac actgaagttt
    15601 aatgagattt tcttgaggca taaaaacgtt aaaacttaaa aaaattaacg acgctattaa
    15661 ttacaaatta taaattagca acgatatgct aaattaaaaa attaaaaagt gcggcaatgt
    15721 ttaatttgat tattaactaa atttcttagc gttttattag tttattttta cgaccattca
    15781 ctaataaatc atttttatta gccaattgca tattttttaa taaaaattat tgatggcttt
    15841 taaaaatggc cggagccgat ggggaggatt gaactcccga ccccgttctt accaagaacg
    15901 tgctctacca ctgagctaca tcggcttttt caacgattgc tcaagattat tttagcaaaa
    15961 aaataaaaaa agacaagaaa aactattctt aattaagttt gcacgttaaa cattttgaat
    16021 ttgatttcta attttatttt agtgggtgga gcaggattcg aacctgcgca gccactaggg
    16081 cgacggtttt acagaccgtt gcaattgacc actctgccat ccaccctacc tatccatata
    16141 aatattgtaa taaactggaa ttgtccatga gccgatggtc ggattcgaac cgacaacctt
    16201 cgctttacaa aagcgccgct ctgccagttg agctacatcg gcgcctca
//
LOCUS       DAOSMC010000011        14533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_4868_length_14533_cov_20.232974, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000011 DAOSMC010000000
VERSION     DAOSMC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4868_length_14533_cov_20.232974"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..74)
                     /locus_tag="PL075_01735"
     tRNA            complement(3..74)
                     /locus_tag="PL075_01735"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Asn,seq:gtt)
     gene            870..4028
                     /locus_tag="PL075_01740"
     CDS             870..4028
                     /locus_tag="PL075_01740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOD96944.1"
                     /translation="MAKFTHLHVHSHYSLLDGLAKIDELINRAKELNFESLALTDHGA
                     MYGIPEFYKTATANGIKPIIGVEVYVAPESRFQKRPKIDSRNFHLTLLAKNEKGYKNL
                     LQLVTRSHLEGFYYKPRVDKELLSEYHEGVIALSGCLAGEISRLIIANRLDEAEEKIK
                     EYQKIFGEDFYLEINYHPKIEESKMIKEQLIRLSKKLSIPAVATYDTHYLKSEDAEAH
                     DILLAVQTGNKIFEEDRLTLSADDFSFCSTEHMEELFYDFPEAIENTRLIADACNVEL
                     EFGKTLLPYFEVPLGETPESHLRKLCEENLPKRFPKITPEINDRLNYELGVIEKTGFA
                     SYFLIVEDFVNWAKNNGIVVGPGRGSAAGSLVAYLLNITDVDPLKYNLLFERFLNPER
                     LQMPDIDMDFADTRRDEVVGYLKNRYGENRVAQIITFGTMASRAAVRDTGRALGLSYS
                     FCDTIAKLIPFNATLDEALTNVIELKNLYERDQDVKKLIDAAKKLEGVARHASIHACG
                     IVITKEEIVNYTPLQRAPQDENSIITQYDMYSLESLGLLKMDLLGLRNLTIIEDALNI
                     IEQTTGEKINFSQLDEKDKKTFELMQKGETVGVFQLESQGMRRYLKELKPTDMEDLIA
                     MISLYRPGPMDLIPHYIARKYGKEKITYLHPKLEPILKSTYGIAIYQEQLLQIAKDLA
                     GFSLGEADLLRRAIGKKIKSLLDAQKQKLIQGMIERDINQDIAEKIWEFIEPFAQYGF
                     NRSHSACYAQIAFRTAYLKAHYPLEFMTALMNNEATETERAAQLINECRRMRITVLPP
                     DLNESFEKFSVVKEKNAIRFGLLAIKNVGENIVKEIIKERERNGRFENIENFLQRICH
                     KDLNKKSLESLTKAGVFDSLAEREKLIANLDNLLSYNQEVRRISLSPQINLFSKELAL
                     PTLTLKETEPMPLEEKLKFEKELLGVYISDHPVRRYEAKFKKLNVRPLNEISEKLVNK
                     KVRVGGVITEIKKILTRNGRSMLAVILEDTSDTAEITVFPDTLEKTLTFWQKDSIIIL
                     EGRVNKNNDHLNIVCEKILAFK"
     gene            4032..5825
                     /gene="thrS"
                     /locus_tag="PL075_01745"
     CDS             4032..5825
                     /gene="thrS"
                     /locus_tag="PL075_01745"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008882264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOD96945.1"
                     /translation="MKQARSKKPRVKKSKNDIEKIRHSLSHLLAAAVLQLYPNVKFGI
                     GPTIENGFYYDFDFGKTKIDESLLPKLEKMMKDLIGRNLKFKKEIVSYARAKKIFRNQ
                     PYKLELLEEFKKQKKPLTIYRTLYGQKEVFVDLCAGPHVASTKIIDKESFKLEKLAGA
                     YWKGDEKNAMLTRIYGLAFHTKEELNDYLHLLEESKKRDHRTIGQQMDLFSFHEEFGP
                     GLVYWHPKGGLIRVLMENFWREAHLKNGYGIVFTPHIGKSDLWKISGHLNFYRENMYS
                     PMKIDADEYYIKPMNCPFHIQIYKSRTRSYRDLPLRWAELGTVYRYEKAGVLHGLLRV
                     RGFTQDDAHLICTPEQMPSEIKKVLNFSLYILKSFGFNDFSIYLSTKPKGSIGPQKHW
                     NEATHALERAIQTSKLPYKIDKGGGAFYGPKIDIKVKDALDREWQCSTIQFDFNMPEK
                     FQMKYTDKNGKQQQPYMIHRALMGSLERFFGALIENYAGAFPFWLMPIQIKLLPVTDK
                     FSPFAYKLKDKLENEGLRVEVDDSNETISKRIRNAEVEKIPYIIVLGQKELESGNLPI
                     RERGSKEIKLISPEAFIELCKNQIPIPQKIQ"
     gene            5786..6811
                     /gene="miaA"
                     /locus_tag="PL075_01750"
     CDS             5786..6811
                     /gene="miaA"
                     /locus_tag="PL075_01750"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOD96946.1"
                     /translation="MQKSNSHTTKNSINSFLQKNNQSAFSVGDFKKPKIVVILGPNAS
                     GKSDLAVLIAKKFNGEIISADSRQVYKGMDIGTGKVPRDNKKFNKNLTLTNEKLYFYQ
                     GIRHHLIDVCSPKKFFTVVEYQKLAQKCIKNILKRKKLPIICGGTGFYIDAALYDYHF
                     PPVKPDWLLRKKLEKLNTKELFAQLQKLDPQYAKRIDPHNKRRLIRAIEIVSILKKPM
                     PPLEKSSHYEVLKIGVYRSPQELKKRIEKRLLERLNQGMIEEVKTLHKKGVSWKRLDN
                     FGLEYRYISLYLRNKISYEEMINQLKTKIWQYARRQMIWFKKDKEIHWISSSSQALKL
                     VRRFLKN"
     gene            complement(6808..7224)
                     /locus_tag="PL075_01755"
     CDS             complement(6808..7224)
                     /locus_tag="PL075_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96947.1"
                     /translation="MEEIRPIKPDKDPLEEAREKEEEVIKNAPPYGGDWEGEYDPLTG
                     KIRIIEPKSEKQKIAEKEFEEEWKQLEEQRQRILKEIEEKEREMKARVQGGLSANDRN
                     RLQKMAEEAKEMLAHIEELEKQLKEIELRLKEIKES"
     gene            complement(7250..8743)
                     /gene="gatB"
                     /locus_tag="PL075_01760"
     CDS             complement(7250..8743)
                     /gene="gatB"
                     /locus_tag="PL075_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOD96948.1"
                     /translation="MIKYTPTIGLEIHIELKTKQKMFCDCLNDPEEKKPNLNICPICL
                     AHPGVLPKPNKEAMEKVLQLGLALNCYLAKIAKFDRKNYFYPDLPKGYQISQYDKPFC
                     QNGYLEISDIEEELYRFKKISIRRIHLEEDTARLIHEGSSSFVDFNRAGIPLAELVTE
                     PEIHSAKEARRFCEELHLIIKYLDIGDADMEKGEMRCEVNISIAADEPNGASKVLGTK
                     VEVKNLNSFRAVEEAIEYEIKRQTELLEKGEKIIQETRGWNEKEKKTISQRIKEEAQD
                     YRYFPEPDLKPIKTAEAFDLDELQRAIPELPNDKRLRFLKEYGLKAKEVEELIYNPPI
                     ADYFEKVIMELDGLKKIEGREREERIKLAYNYLNTDLKSLLLQNNVAVEEIKISPHHF
                     ALLIDFLDKKVISSAAGKAVLAEIFESGASPEVVIKEKNLLQVFDTKEIDEIVKKVVA
                     ENPQAVADFQKGKANALQFLVGQVMKESKGRFNPQLVQERINALLTK"
     gene            complement(8763..9281)
                     /locus_tag="PL075_01765"
     CDS             complement(8763..9281)
                     /locus_tag="PL075_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96949.1"
                     /translation="MKLVKSEEPVKKIVFCSSQRFRSELLEFINEFREIVRKNQKPFM
                     ILEPDFDTTPKEILNLSEKERLQNSAEYRERLIWDAANHLFRRVKIADIVFIFNKNGY
                     IGVNTSGELFAAAVLDKKIYSLEKKILMGHYPDDLYEEPFVSFLVWDVAQTPQEFFNK
                     ICKDFEKKKKIR"
     gene            complement(9560..9976)
                     /locus_tag="PL075_01770"
     CDS             complement(9560..9976)
                     /locus_tag="PL075_01770"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOD96950.1"
                     /translation="MRHCNHQSVGIIIKKNEKMLLVDRKKFPYYWASVAGHIEDDELP
                     NAAARREVKEEVGLKIRGLVPLLNKKRFNNPCHRGSTFHYWWVYLADCTGKVRIRKKE
                     VKRFGWFTKEEIKQMIAKKKIEPVWVKIFQKTKAFK"
     gene            complement(10077..10904)
                     /locus_tag="PL075_01775"
     CDS             complement(10077..10904)
                     /locus_tag="PL075_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96951.1"
                     /translation="MKKIPYAKISDGGKFILIEGLNGSGKTTQAKLLERALAEEGKIA
                     LYNHEPTDGLLGKIIRAIIEKRPIIESEVEIENFLEKFFPKSNPHSDAILQATKVVLK
                     QFQNGKVRNEEAKRQLLFIIDRLFDIKEIIQPALRQNHWVVQDRYDISCYLHGMANGL
                     DFNYLTKHHARILGENYLAPNVIIFYWVPVKVALQRLVDSGKTIDLYENTQTLKKIEK
                     AARWLFSFRFQSPSPGHPLRRKLQVGNKKVRVFIVNAEAPIAEIFEETWGILENIKI"
     gene            complement(11124..11549)
                     /gene="rplK"
                     /locus_tag="PL075_01780"
     CDS             complement(11124..11549)
                     /gene="rplK"
                     /locus_tag="PL075_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOD96952.1"
                     /translation="MAKKIKSIIKLQIPAGQANPAPPVGTALGPQGINIGEFCQKFNE
                     ATKDQAGDVIPVEITIYEDRSFEFKLKTSPAADLLKKAANISKGSAEPNRKKVGKVTR
                     AQIREIAEKKMVDLNANDIEAAVKIIEGTARNMGIEIID"
     gene            complement(11681..12226)
                     /gene="nusG"
                     /locus_tag="PL075_01785"
     CDS             complement(11681..12226)
                     /gene="nusG"
                     /locus_tag="PL075_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOD96953.1"
                     /translation="MAKQTPEEKRWYAIHTYSGYEDAVAHYLKQRVDSLDMHDKIFNI
                     IVPKEKKIRIKGGKRYVTEEKIYPGYVLVEMILTDDSWYVVRNTPRVSGFIGADATKP
                     SPLSDEEVQALLKRMNVEEPKYKVDLQVGDMVKIIDGPFKDFDGKVKEVDEATAKIKV
                     LVNIFGRETPVELDFLQVKKT"
     gene            complement(12230..12415)
                     /gene="secE"
                     /locus_tag="PL075_01790"
     CDS             complement(12230..12415)
                     /gene="secE"
                     /locus_tag="PL075_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOD96954.1"
                     /translation="MFKKIKPYFKEVKQEFKHVNWLTKKEAIKYTVMVIGFSVAMSVF
                     LGFCDFAFMQILSQIII"
     gene            complement(12446..14440)
                     /gene="gyrB"
                     /locus_tag="PL075_01795"
     CDS             complement(12446..14440)
                     /gene="gyrB"
                     /locus_tag="PL075_01795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOD96955.1"
                     /translation="MSKKKTEKVDLQKQISNELQQQNESDKPTSYNAKDIVVLEGLEP
                     VRKRPGMYIGSTGIDGLHHLIYEVVDNAIDEAMAGYAKNIEVRLYDNHRVSVQDDGRG
                     IPVDIHPQTKKSSLETVMTILHAGAKFGGASYKVSGGLHGVGVSVVNALSSYLKAEVC
                     RDGQKYYQEYSKGKPKTKLIKMGSCRISGTTIIFEPDVEIFATKGAKMPEYDFKRLAD
                     HLREQAYLTKGVRIRIVDGRTSKENIADFYFEGGVISFIKYLIEEERPIQDNVFYAAK
                     NYDKIFVEAAFCYTEDIQATEISFANNIRTPEGGMHLTGFRTALTRVLNDWAKKEGYL
                     KDGEDGLTGDDAREGLTAIISVKLPDPQFEGQTKEKLGNPEVRTAVDAVVSEALKEFL
                     EKNPNDAKRILEKCILAAKARRAAKAAKEMVLRKGLLEGLSLPGKLTDCSTRHPEEAE
                     LFIVEGDSAGGSAKQGRDRRFQAILPIKGKILNVEKARLNKIMESQEIKSIIIALGTA
                     IAEEFDINKLRYHKIIIMTDADVDGAHICSLLLTLFFRFLPEVIHRGHLYIAQPPLYR
                     IQKGKEFYYAYNEEQKDQILKKLGSENISIQRYKGLGEMNPEQLWETTMNPESRILKK
                     VTIEDAEEADKLFTILMGDEVAPRKHFIQVHAKTVDNLDI"
BASE COUNT         4583 a         2733 c         2391 g         4826 t
ORIGIN      
        1 ggctccggtg gtaggattcg aacctacaac catctcctta acagggagcc gctctaccgt
       61 tgagctacac cggaatattc ccattttaaa aaagaaaaat ggaaaatgca agaagaaaat
      121 taagttaaaa ctgcgaattc ttcgcccgct accaacaagc agggtttcgc tcaaaatgac
      181 aaccgtatta ctgtcatcct atttttattg tcatcctgag cgcctgccca ccgataggtg
      241 ggaagcgaag gatctctcgg cttttagcaa taattggcca tcactgctgt ggatgggatt
      301 cttcggactt cgtcctcaga atgaccgtgg gggttttgct gtggatggga ttcttcgctg
      361 acgctcagaa tgacaaaaaa taaaacgctc agaataatta aacttttgaa tttttaattt
      421 taaaatttgg gatttaaaat ttgtattttc attagttgac aaaaatattt atttaggcta
      481 aaattgaaaa tagaggtgaa agccttaaaa attttataag gaggagaaga agatgagtaa
      541 atcaaaaaaa cgagtgattt acgtggaaac tgaagtgaag tgccccaatt gcaataattt
      601 cttactggaa gcagtcaaag tcccgcacga attctgcgga caacgagaag aagaagatct
      661 tcttgttggt aaaagactcg gctactattg ttcagagtgc ggagagaaat acgactccga
      721 ttttgcagta ctcgacttgc agcaagagac tgactaaaaa caaacaggga ctcctctaac
      781 ggagtccttt ttatttttct aaaaatttgc tttaaaaatt tttctaaata aaatttttgt
      841 gttaaaattg aaaagataaa taaaatttta tggcgaaatt tactcattta catgttcata
      901 gtcactattc ccttcttgat ggtttagcta aaattgatga attaattaat cgcgcaaaag
      961 aattaaattt tgaatcatta gccctgactg atcatggcgc tatgtatggc atcccagaat
     1021 tttacaaaac tgccacagca aatggaatta aaccaattat tggggtggag gtttatgtgg
     1081 cgccagagag tcgttttcaa aaaagaccaa aaattgatag ccggaatttt cacttaacat
     1141 tattggctaa aaacgaaaag ggttataaaa atctattgca attagtaaca cgctctcatt
     1201 tagaaggctt ttattataaa ccgcgcgtag ataaagaatt gctttcagaa tatcatgagg
     1261 gtgttattgc tttgtccggc tgtttggctg gcgaaatttc acgtttaatt attgccaatc
     1321 gtcttgatga agccgaagaa aaaattaaag aatatcagaa aatctttggg gaagattttt
     1381 atttagaaat taattatcac ccgaaaatag aagaaagcaa aatgataaaa gagcaactaa
     1441 ttcgtttaag taaaaaatta agcattcccg ctgtagctac ttatgatacg cattatttaa
     1501 aatcggaaga tgcggaagcg catgatattt tattagccgt tcaaaccggt aataaaattt
     1561 ttgaagaaga tcgacttaca ctcagcgcag atgatttttc gttttgctca acagaacaca
     1621 tggaagaatt attctatgat tttcctgaag caattgaaaa cactcgactc atcgccgatg
     1681 cttgtaatgt tgagttggaa ttcgggaaaa cgctacttcc ttattttgaa gtgccactcg
     1741 gcgaaacacc ggaatcacat ttgagaaaac tgtgcgaaga aaatttaccc aaaagatttc
     1801 ccaaaatcac accagaaatt aacgatcgat taaattatga actaggagta attgaaaaaa
     1861 ctggctttgc ttcttatttt ttaattgttg aggattttgt gaattgggcc aaaaataatg
     1921 gcattgtagt aggcccagga cgtggctcag ctgctggatc gcttgtggct taccttttaa
     1981 acatcaccga tgttgatccg ctcaaatata atttattatt tgaacgcttt ttaaaccctg
     2041 aacggcttca gatgcctgat attgatatgg attttgctga tacacgccgc gacgaagtag
     2101 ttggctattt aaaaaataga tatggtgaaa atcgagtggc tcaaataatt acctttggaa
     2161 caatggcttc ccgcgctgcc gtacgcgaca ctggccgagc tttggggctt agttatagct
     2221 tttgcgacac aattgccaaa ctaatccctt ttaatgcgac gctggatgaa gcgcttacaa
     2281 atgttattga actaaaaaat ttatacgaac gtgatcagga tgttaaaaaa ttaattgacg
     2341 ctgcaaaaaa attagaaggt gtcgcaaggc atgcctctat tcacgcttgc ggtattgtta
     2401 ttactaagga agaaattgtt aattacactc cgctacaaag agcgcctcaa gatgaaaact
     2461 ctattattac tcaatatgat atgtattctt tggaatcgct tggtcttttg aaaatggatc
     2521 ttttgggatt aagaaattta actattattg aggacgcgct caatattatt gaacaaacga
     2581 ctggtgaaaa aattaatttt agccaacttg atgagaaaga caaaaaaaca tttgaattaa
     2641 tgcaaaaagg agaaaccgtg ggtgtttttc aattagaaag tcagggcatg cgacggtatc
     2701 tcaaagaatt aaaacctact gacatggaag atctcattgc aatgatttct ctttatcggc
     2761 cgggtccaat ggatttaatc ccccactata ttgctcgcaa atacggcaaa gaaaaaataa
     2821 cttatcttca tccaaaactg gaacccatcc ttaaatcaac ttatggcatt gctatttacc
     2881 aggaacaatt attacaaatt gctaaagatt tagctggttt tagtttagga gaagccgatt
     2941 tgttgcgccg cgccattggg aaaaaaatca aaagtcttct tgatgcccaa aaacaaaaat
     3001 taattcaagg aatgatagaa cgtgacatca atcaagatat tgccgaaaaa atttgggaat
     3061 ttatagaacc atttgctcaa tatggtttta atcgcagtca tagcgcttgt tatgctcaaa
     3121 ttgcctttcg cacggcttat cttaaagctc attatccttt agaatttatg acggctttaa
     3181 tgaataacga ggccactgaa acagaacgag ccgctcaact aataaacgag tgtcgtcgaa
     3241 tgcgcattac tgttttgccg ccagatctta acgaaagctt tgaaaagttt tcggtagtaa
     3301 aagaaaaaaa tgctattcgt tttggtcttt tagcaattaa aaacgtgggt gaaaatattg
     3361 ttaaagaaat tattaaagaa agagaaagaa atggtcgctt tgaaaacatt gaaaattttc
     3421 ttcaaagaat ctgtcataaa gatttaaata aaaaatcatt ggaaagccta acaaaagccg
     3481 gagtttttga cagtcttgcc gaaagagaaa agctcatcgc taatttagat aatctacttt
     3541 cttataatca agaagtgagg cggataagcc ttagtcctca aattaatctt ttctcaaaag
     3601 aattagccct acctactcta actctcaaag aaaccgaacc tatgccgctt gaagaaaagt
     3661 taaaatttga aaaagaactc ttgggtgttt atatttctga ccatccggtt cgtcgctatg
     3721 aagcaaaatt caaaaaatta aatgtacggc ctctgaacga aatctccgaa aaattagtca
     3781 ataaaaaagt tcgcgtcggt ggcgttatca cagaaataaa aaaaattcta acgcgtaatg
     3841 gtcgttctat gctcgctgtt atcctagaag atacatctga tacggccgaa attacagttt
     3901 tcccagatac tcttgaaaaa acattaacat tttggcaaaa agatagtata attattcttg
     3961 aaggtcgtgt aaacaaaaac aatgatcatt tgaatattgt ttgcgaaaaa attttagctt
     4021 ttaaataacc tatgaaacaa gctcgttcta aaaaacctcg cgtaaaaaag tcgaaaaatg
     4081 atattgaaaa aatcagacat tctttgagtc atttgttagc agcggctgtt ctgcaactct
     4141 atcccaatgt aaagtttggg atcggaccaa ctattgaaaa tggtttttat tatgattttg
     4201 attttggaaa aacaaaaatt gatgaatcgc tgcttcctaa attagaaaaa atgatgaaag
     4261 atttaattgg ccgtaatctc aaatttaaaa aagagattgt ctcttatgct cgagcaaaaa
     4321 aaattttcag aaatcaaccc tataaattgg aattgcttga agaattcaaa aaacagaaaa
     4381 aaccgcttac tatttataga actctttatg gtcaaaaaga ggtttttgtt gatctttgcg
     4441 ccgggccaca cgtcgcttct acgaaaatca ttgataaaga atcttttaaa ttagaaaaat
     4501 tagcaggcgc ttattggaag ggtgatgaaa aaaatgcgat gctcacccga atttacgggt
     4561 tggcgtttca taccaaagaa gaacttaacg attatttaca cttgcttgag gaatcaaaaa
     4621 aacgcgatca tcgaactatc ggtcaacaaa tggatctttt tagcttccat gaagaatttg
     4681 gtccaggtct ggtttattgg caccccaaag gcggattgat tagagtgctg atggaaaact
     4741 tctggcgaga agcacactta aaaaatggtt atggaattgt ttttacaccg cacatcggca
     4801 aatctgatct ctggaaaatt agcggccact taaactttta tcgagaaaat atgtattcac
     4861 cgatgaaaat cgatgcagat gaatattata ttaaacccat gaattgccca tttcatattc
     4921 aaatttacaa atcgcgaacg cgatcctatc gcgatttgcc attgcgttgg gctgaattgg
     4981 gaacagttta tcgatatgaa aaggcgggcg ttcttcatgg attgcttcga gtccgcgggt
     5041 tcacacaaga cgatgctcat ttaatttgca cgccagaaca aatgccctca gaaattaaaa
     5101 aagtactaaa tttctcactc tatattttga aaagctttgg ttttaatgat tttagcattt
     5161 atctctctac caagccaaaa ggttctattg gccctcaaaa acattggaat gaggccactc
     5221 atgccttaga gcgagctatc caaacttcta aattacctta caaaattgat aaaggaggcg
     5281 gtgcttttta tgggccgaaa attgatatta aagtaaaaga cgcgctggat cgagaatggc
     5341 aatgttctac tatccaattt gatttcaata tgccggaaaa attccaaatg aaatatacag
     5401 ataaaaatgg caaacagcaa cagccttata tgattcatcg cgctctaatg ggatcattag
     5461 aaagattttt tggcgcactc attgaaaatt atgccggtgc ctttcctttt tggctgatgc
     5521 ccattcaaat caaattatta cccgtaactg ataagttttc gccttttgct tataaactta
     5581 aagataaact agaaaacgaa gggctgcgcg ttgaggtgga tgatagcaat gaaacaatta
     5641 gtaaacgcat tcgcaatgct gaagtagaaa aaattcccta tattattgtt ttaggtcaaa
     5701 aggaattgga atcgggaaac ttgcctattc gtgaacgagg cagcaaagaa attaaattga
     5761 tttcgcccga agcatttatt gaattgtgca aaaatcaaat tcccatacca caaaaaattc
     5821 aataaattct tttttacaaa aaaataatca atcggctttt tcagtcggtg attttaaaaa
     5881 accaaaaatt gtcgtaattt tgggacctaa tgcttctggc aaatcggact tggctgttct
     5941 aatagctaaa aaatttaacg gcgaaatcat ctcggctgat tcaaggcaag tttataaagg
     6001 aatggatatc ggtacaggaa aagtacccag agacaataaa aaatttaata aaaatctaac
     6061 attaacaaac gaaaaattgt atttttatca aggaattaga caccatttga ttgatgtttg
     6121 ttctcccaaa aaatttttta ccgttgttga atatcaaaaa ctagcccaaa aatgtataaa
     6181 gaatatttta aaaagaaaaa aacttcctat tatttgcgga ggtacgggtt tttatattga
     6241 tgccgctctc tacgattatc actttcctcc ggttaaacct gattggctct taagaaaaaa
     6301 gttggagaaa ttaaatacta aagaattatt cgctcaactt caaaaacttg atccccaata
     6361 cgccaaaaga attgaccctc acaataaaag aaggctgatt cgcgctattg aaattgtttc
     6421 tattctcaaa aagcctatgc caccattaga aaaatcttcg cattatgaag ttttaaaaat
     6481 tggagtttat cgttccccac aagaattaaa aaaacgaata gaaaaacgct tgttagaaag
     6541 acttaatcaa ggaatgattg aggaagttaa aacactgcat aaaaaaggtg ttagttggaa
     6601 gcgcttggat aattttggtt tggaatatcg ttatattagt ttatatctcc gaaataaaat
     6661 ttcttacgag gaaatgatta atcaattaaa aacaaaaatc tggcaatatg ctcgccgcca
     6721 gatgatttgg ttcaaaaaag ataaagaaat tcattggatt tcttcttctt ctcaagcatt
     6781 aaaacttgtt cgccgatttc ttaaaaatta agattctttt atttctttca gtctcaattc
     6841 aatttctttt aattgttttt ccagttcttc aatatgagca agcatctctt tggcttcctc
     6901 tgccattttt tgtaaacgat ttctatcgtt agcagataac ccgccttgca cgcgcgcctt
     6961 catttccctt tctttttctt caatctcttt aagaattctc tggcgttgtt cttctaattg
     7021 cttccattct tcttcaaatt ctttctcagc gattttctgt ttttcgcttt taggttcaat
     7081 aatcctgatc ttaccagtta aaggatcgta ctctccttcc caatcgcctc cataaggtgg
     7141 cgcatttttt atcacctctt cttccttttc tcgggcttct tctaatggat ctttatcggg
     7201 tttgattgga cgtatttctt ccatatattt atattatagc aaaacgattt tactttgtca
     7261 agagagcatt aattctctct tgaactaatt gaggattaaa tcgaccttta ctttctttca
     7321 tcacttgccc cactaaaaat tgtaacgcat tggctttgcc tttttgaaaa tcagcgactg
     7381 cttgcggatt ttcagcaaca acttttttaa caatctcatc aatttctttg gtatcaaaaa
     7441 cttggagtaa gtttttttct ttaatcacca cctcgggact agcgccgctt tcaaaaattt
     7501 ctgctaacac agccttgcca gccgctgagg aaatcacttt tttatctaaa aaatcaatga
     7561 gcagagcgaa atgatggggg gaaattttaa tttcttccac tgcaacatta ttttgaagaa
     7621 gcaacgactt caagtcagta tttaaatagt tataagccaa tttaattctt tcttctcttt
     7681 ctcttccttc aatttttttt aacccatcta attccataat gactttttca aaataatcgg
     7741 ctattggagg attgtaaatt aattcttcca cttctttagc ttttaatcca tattctttta
     7801 gaaatcttaa tcttttgtcg ttgggcaatt caggaatagc tcgctgcaac tcatctaaat
     7861 caaatgcttc cgctgttttt attggtttta aatccggttc ggggaaataa cgataatcct
     7921 gtgcttcttc cttgatacgc tgagaaatag tttttttctc tttttcattc caccctcgcg
     7981 tttcctgaat aattttttca cccttctcta acaattctgt ctgacgtttt atttcatatt
     8041 caatggcctc ctcaactgct cgaaacgaat tgagattttt aacctcaacc ttcgtgccta
     8101 aaactttaga agcgccgttc ggttcatcgg cggcaatact aatatttact tcacaacgca
     8161 tctccccttt ttccatatca gcatctccaa tatctaaata tttgatgata agatgcaatt
     8221 cctcacaaaa tcttcttgct tctttagcag aatgaatttc gggttcagta actaactctg
     8281 ctaaaggtat gccggcgcga ttaaaatcaa caaaagaact gcttccttca tgaattaaac
     8341 gagcagtatc ttcttctaga tgaatacgcc gaatggaaat ttttttaaac cgatagagct
     8401 cttcttcaat atcactgatt tctaaatagc cattctgaca aaatggtttg tcgtattgag
     8461 aaatttgata acccttgggc aagtcagggt agaaatagtt tttacgatcg aatttagcaa
     8521 ttttagctaa atagcagttt aaagctaatc caagttgtaa aactttttcc atcgcctctt
     8581 tattgggttt tggtaaaacg ccgggatgag ctaaacaaat cgggcaaata tttaaatttg
     8641 gctttttctc ttctgggtca ttcaagcaat cacaaaacat tttttgtttt gttttgagtt
     8701 caatatgaat ttctaaacca attgtgggtg tatatttaat cattgtttat ttttaataaa
     8761 aattacctga ttttcttttt cttttcaaaa tctttacaaa ttttattaaa aaattcctga
     8821 ggagtttgtg ctacgtccca aacaagaaaa gaaacaaatg gttcttcgta taaatcatca
     8881 gggtagtgcc ccataagtat tttcttttct aaagagtata tttttttatc aagaacagca
     8941 gcggcaaata attctccact tgtattcacg ccgatatagc catttttatt aaaaatgaag
     9001 acgatatcag caattttgac gcggcgaaat aaatgattgg cggcatccca aattaatcgt
     9061 tcgcgatatt ccgcgctgtt ttgcaaacgt tctttctctg aaagatttaa aatttctttg
     9121 ggcgtcgtat cgaaatcagg ctctaaaatc ataaaaggct tctgattttt tctaacgatt
     9181 tctcgaaatt cattaataaa ttctagaagt tctgaacgaa aacgttggct ggaacaaaaa
     9241 acaatttttt ttactggctc ctccgacttt accaatttca tactacattc ttattgtata
     9301 aaaaattttc ccaaactgca acaaaaaata acaaaaccga aaataaaata acaagagcac
     9361 cggatgaaaa ttcgaaataa ataaaaaaat gcatctctga ataatcactt aacgattgcc
     9421 gacgattaaa ataagtccga actctaaatt aacacataag atcattcaac tttttttacg
     9481 tgggaatagg tgggaaagcg gaaaagtttc caaaggaaat ttttccgctt tccccaattt
     9541 tacgattaaa aattggcatc tacttaaatg cttttgtttt ctgaaaaatt tttacccaaa
     9601 cgggttctat ttttttctta gcaatcatct gtttaatttc ttcttttgta aaccaaccaa
     9661 atcgctttac ttcttttttt ctgattctta cttttccggt acaatcagcc aaataaaccc
     9721 accagtaatg gaaggtgctt cctcgatgac acggattatt aaatcttttt ttattcaaca
     9781 aaggcactaa tcctcttatt tttaatccaa cctcttcttt aacctctcgt cttgctgctg
     9841 catttggcaa ttcatcatct tcaatatggc ctgccaccga agcccaataa taaggaaatt
     9901 ttttccgatc aaccaaaagc atcttttcat tctttttgat aatgatccct acgctttgat
     9961 gattgcaatg tctcataatc aaataattta atctttttga tattatatag ttttttatta
    10021 ttttgccaaa ttttcttcaa taaaatagcc ctgaattttt cagggctata ttgttatcat
    10081 atttttatat tttctaaaat tccccatgtt tcttcaaaaa tttctgctat cggcgcttca
    10141 gcattaacaa taaaaactct aactttttta tttcctactt gcaatttccg tctcaaggga
    10201 tgaccgggcg aaggactttg aaaacgaaaa ctaaataacc accttgctgc cttttcaatt
    10261 tttttcaacg tctgtgtgtt ctcgtaaaga tcaatcgtct tccctgaatc aaccaaccgc
    10321 tgtagggcca cttttaccgg tacccaataa aaaattatca cattgggtgc caaataattt
    10381 tcgcctaaaa ttcgggcatg atgtttggtc agatagttga aatctaatcc gtttgccatt
    10441 ccatgcagat agcacgaaat atcatatcta tcctgcacta cccagtgatt ctgccgtaga
    10501 gctggctgaa taatctcctt gatatcaaat aaacgatcaa taataaaaag gagctgtctc
    10561 tttgcttctt catttcgcac tttgccattt tgaaattgtt ttaaaactac ttttgtagct
    10621 tgcaaaatag catcgctgtg gggattgctt ttgggaaaaa atttctccaa gaaattttcg
    10681 atttctactt ctgattcaat aattgggcgt ttttcgataa ttgctctgat tattttcccc
    10741 aacaagccat cagttggctc atgattgtat aaagctattt ttccctcttc tgccaaagcc
    10801 cgctctaata atttcgcctg ggtcgtttta cccgacccat ttagcccttc aattaaaata
    10861 aatttccctc catcactaat tttagcataa ggaatttttt tcataacaaa cctcctttag
    10921 attttttaaa agctgagaat gattttaata taattttact atctgtcaat aattttcttc
    10981 tttaatttca atatctttaa tctttttctt cgatcaattc atttcttata tattattgca
    11041 ggtttcatta tttaattcaa ttgtagaaaa ataaaaatcc cgccatttgg agttttgacg
    11101 agatttatga aaacgagttg tttttaatct attatctcaa tgcccatatt tctggcggtg
    11161 ccttcgataa ttttcaccgc tgcctcaata tcattcgcgt tgagatctac cattttcttt
    11221 tcggcaattt ctcgaatttg agcgcgcgtc acctttccta ctttctttct atttggttcg
    11281 gctgatcctt tgctaatatt ggctgctttt ttgagcaaat cagcagccgg agaagttttt
    11341 aatttgaatt caaacgatcg atcttcgtaa atagtaattt ccactggaat aacgtctcca
    11401 gcttggtctt tagtagcctc attaaatttc tggcaaaatt cgccaatatt aattccttga
    11461 ggacctaaag ctgtgcccac cggtggcgca ggattggctt gaccagctgg tatctgaagt
    11521 ttaattattg atttaatttt tttagccata atagttatta gtttttagtt gctggttttg
    11581 gttgtctgtc ttttattttt taaaatagtc gacttttatt ttttatactt ccgccttccc
    11641 attaaactct ataaaaatat gttttgcaaa attgtataaa ttaggtcttt tttacttgga
    11701 gaaaatctaa ctctactggt gtttcgcgac caaaaatatt tactaaaacc tttattttag
    11761 ctgtggcctc atcaacctct ttcaccttcc cgtcaaaatc cttaaatggt ccatcgataa
    11821 tttttaccat atctcccacc tgtagatcaa ctttatattt tggctcttcc acattcattc
    11881 ttttaagcaa cgcctgcact tcttcgtcag ataaaggact cggcttggtt gcgtcagctc
    11941 caataaatcc cgaaacgcgt ggcgtattgc gcactacata ccaggaatca tcggttaaaa
    12001 tcatttctac taaaacataa cccggataaa ttttttcttc tgtcacatat cttttacctc
    12061 ctttaatcct aatctttttt tctttcggta caataatatt aaaaatttta tcgtgcatat
    12121 ctaaagaatc cactctttgt ttcaaataat gagcaacggc atcttcatag cccgaataag
    12181 tatgaatggc ataccatctt ttttcttctg gtgtttgttt agccataaat taaataataa
    12241 tctgacttaa aatttgcata aaagcaaaat cgcaaaagcc taaaaaaacc gacattgcca
    12301 cggaaaaacc aattaccatt acagtgtatt ttatggcctc ttttttggtc aaccaattaa
    12361 cgtgtttaaa ttcttgctta acttctttaa agtatggctt aattttttta aacataagat
    12421 tatttaaaat attaaaattt aaatgctaaa tgtcaaggtt atcaaccgtt ttagcgtgaa
    12481 cttgaatgaa atgtttgcgt ggcgcaactt catcgcccat caaaatagta aatagtttgt
    12541 ctgcttcttc agcatcttca atagttactt ttttcaaaat tctactttcg ggattcatcg
    12601 ttgtttccca taattgttcc ggattcattt cgcctaaacc cttgtagcgc tgaatactaa
    12661 tattttcact acccaatttt tttagaattt gatctttctg ctcttcgtta taggcataat
    12721 aaaactcttt gcctttttga atgcgataaa gcggtggttg tgcaatataa agatgaccgc
    12781 ggtgaatgac ttcgggtaaa aagcgaaaaa atagcgttaa aagcaaagaa caaatatgag
    12841 cgccgtcaac atctgcatca gtcataataa taattttatg atagcgtaat ttattaatat
    12901 caaactcttc ggcaatagct gttcccaaag caataataat agatttaatt tcttgtgatt
    12961 ccatgatttt atttaaacgc gctttttcta cattaagaat tttaccttta attggcagaa
    13021 tggcttgaaa acgacgatcg cgcccttgtt ttgctgaacc gccagctgaa tcaccttcaa
    13081 caataaaaag ttctgcttcc tctgggtggc gtgttgaaca atctgttaat ttgcccggca
    13141 ggcttaaacc ttcaagcaat cccttgcgaa gaaccatttc tttagcagct ttagcggctc
    13201 ttctcgcctt agcagctaaa atacatttct ctaaaattct tttggcatca ttcggatttt
    13261 tttctaaaaa ctctttgaga gcttcgctta caacagcgtc aactgcagtc cgaacttccg
    13321 gattacctag tttttctttt gtctgacctt caaattgagg atcgggcagt ttaacagaaa
    13381 taatggctgt taatccttcg cgcgcatcat ctcctgttaa tccatcttct ccgtctttga
    13441 gatacccctc ttttttagcc cagtcattaa gtactcgagt taatgctgtt cgaaagccag
    13501 tcaaatgcat tccgccttct ggagtgcgaa tattattagc aaatgatatt tctgttgctt
    13561 gaatgtcttc tgtataacaa aatgcggctt caacaaaaat tttgtcataa tttttggccg
    13621 cataaaaaac attatcttga attggccttt cttcttcaat aagatattta ataaaagaaa
    13681 tgacgccgcc ttcaaaataa aaatcagcga tgttctcttt gcttgttctg ccatcaacta
    13741 tcctgattct tacacctttc gtcagatagg cttgttctcg caagtgatca gctaagcgtt
    13801 taaaatcata ttcgggcatt tttgcccctt tggtagcaaa aatttctaca tcgggttcaa
    13861 aaataatggt cgtacccgaa atgcgacaac tgcccatttt tattaatttt gtttttggct
    13921 taccttttga atattcttga taatattttt gcccatcacg acaaacttca gcttttaaat
    13981 acgaagaaag tgcgttaacg acagaaacgc ctacgccatg taaaccacca gaaactttat
    14041 acgaagcacc gccaaattta gcaccggcgt gaagaatggt cattacggtt tcaagtgaag
    14101 attttttggt ttgaggatga atatcaaccg gaatacctcg gccatcatct tgaaccgaaa
    14161 cacgatgatt atcataaagc cgtacttcaa tatttttcgc ataaccagcc attgcctcgt
    14221 caattgcatt gtctactact tcataaataa ggtgatgcag gccatcaata ccggttgaac
    14281 caatatacat tcctggtctt tttctgaccg gctccaatcc ttccaaaacc acaatatcct
    14341 tcgcgttata ggaagttggt ttatctgatt cgttttgttg ttgtaattcg ttagaaattt
    14401 gtttttgcaa atctactttc tcggtttttt tcttagacat tctaattata ttttagcaaa
    14461 tttttaagaa aaaataaagg gcggatgtac tctcgtggct ttcaacattg tgtagtataa
    14521 ccgccactgc aat
//
LOCUS       DAOSMC010000012        13554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_5310_length_13554_cov_19.122750, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000012 DAOSMC010000000
VERSION     DAOSMC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13554
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5310_length_13554_cov_19.122750"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PL075_01800"
     CDS             <1..494
                     /locus_tag="PL075_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD96956.1"
                     /translation="DAINIDLKSMQEKFYQKICGAYLEPVLKNIQNFYEKGVWVELTT
                     LIIPNENDSEKELKQIAKFIAEIDQNIPWHISRFFPSYQMENYPMTPMNTLKKAYEIG
                     KENGLHFVYAGNIIEESLENTYCPKCGVLLIERQGYNIKIKDDFRNGRCLNCKAAIKG
                     VWK"
     gene            499..1275
                     /gene="amrB"
                     /locus_tag="PL075_01805"
     CDS             499..1275
                     /gene="amrB"
                     /locus_tag="PL075_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOD96957.1"
                     /translation="MNIRPPVVAGQFYPEDPRELKETIQEFLDQQPKDLFIPQKIRAL
                     VVPHAGYIYSGPVAAAAYQTLRLSRFEPQKIVLIGPSHHFPIADYITSSFDQWKTPLG
                     KVPQIKVTQNEWTVFDLAFMPEHSLEVQLPFLQTILSNFEIFPILINNISLSETLARK
                     IKSLLDEKTLIIVSSDLSHYNPLAITQKIDKQTQEIILQLKTTEAENIDACGAAGILT
                     LMHLAKDLHWQRKLISYATSYDSSNYKDEVVGYGSFLFYE"
     gene            1268..1849
                     /gene="amrA"
                     /locus_tag="PL075_01810"
     CDS             1268..1849
                     /gene="amrA"
                     /locus_tag="PL075_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOD96958.1"
                     /translation="MNNFSLTEKDKKFLLELAHQSIQYFLQHHKIFEIDEEELKNYSP
                     AIKQKRGCFVTLTIDDELRGCIGCFESNKPLYQTVIEMAISAAFFDYRFSPLSKEESK
                     QIKIEISILTPPRELKYNDAADLLKKLKPLKHGVIITKGNYQATYLPQVWEQIPGKEE
                     FLNSLCLKAGLPFDCWKKEKLNVKIYEVESFKE"
     gene            1862..2191
                     /gene="metG"
                     /locus_tag="PL075_01815"
     CDS             1862..2191
                     /gene="metG"
                     /locus_tag="PL075_01815"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOD96959.1"
                     /translation="METINLNDFKKLDLRLAQIIEAERVPDTNKLLKLKLDLGDETRE
                     IVSGIGMQYHPEDLIGKQIVIVANLEPKVFKGIESQGMLLAVVNKDGDISLLIPDKQM
                     TPGDRIQ"
     gene            2288..2494
                     /locus_tag="PL075_01820"
     CDS             2288..2494
                     /locus_tag="PL075_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96960.1"
                     /translation="MSVLALEDLQIVNLAKEDDEDLDELEEDVEEEKEEEKDDDDLDD
                     FNEDDIDEDLFSDDDLEEESEEEL"
     gene            2569..5064
                     /locus_tag="PL075_01825"
     CDS             2569..5064
                     /locus_tag="PL075_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOD96961.1"
                     /translation="MVKRLTESSYIPKKKRLRKTNTRKALFIGVVFLFVAIVIAGIGY
                     IVYLYYTLPDPANVGETMINQSTKIYDRTGEVLLYEIHGEEKRTVIALSDIPLHVQKA
                     TLAIEDAEFYQHPAFNWKSLVRAVFINLKEGRFAQGGSTITQQVVKNIYLTPEKTIQR
                     KIKEILLAVKLEEHYTKDQILEMYLNQIPYGSNAYGIETAAQTYFNKSAKDLTLAEAA
                     ILAALPQAPSYYSPYGNNQKELFDRQKLVLKRMLDLGFITQEEYDTAVNEKIKFAYRI
                     EKINAPHFVMFVKQYLENKYGTDYVENAGLKVITTLDWDLQQIAERVVYEGALRNEKL
                     YKGKNAALIAQDPKTGQILSMVGSRDYFDLDVDGNFNVITGHRQPGSSFKPFAYLTAF
                     EKGLTPETVVFDLKTEFNSNCPATADGDTDRYGLNCYHPQNFDLTFKGPIDLRHALAQ
                     SINVPAVKVLYLAGIKDTIATAQKMGVTTLTDINRYGLSLVLGGGDIKPLDFAEAYSV
                     FAQDGVKHQQAAILKIEDAKGSILEEYKDVSEQVIDPQYIRLINSVLSDKEARIGLFN
                     PGNQLEIPGYEVAAKTGTTQDYRDAWTVGYTPSLVAIVWAGNNDFSAMQKGGGSILAA
                     VPIWHNFMIEALKKYQPETFPQPAPTNPNKPVLRGEYIVNFKIGDIIYPQIHNILFWV
                     NKNDILGPSPLNFNDPQANNWEYPVTQWIQNNLPNPQQYNIVIPYNYAELPQTTLQPT
                     FSIEFINPKNGDYITGGVIYVVLNIQTTNQIKSLRIYFNDNLVREDVAGNGIYQFQFS
                     PLQIEDQNQLKVNICDVLNSCTEKSIVIFKQQI"
     gene            complement(5065..5340)
                     /locus_tag="PL075_01830"
     CDS             complement(5065..5340)
                     /locus_tag="PL075_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96962.1"
                     /translation="MDDIKKIINKKLNQLGIKQKIKEISVINYTAEFLKEHLQQISVE
                     EINFFNHNLTIKVKNAIEANELKFFEEELRFFLAQKGFKINKIKIIF"
     gene            complement(5342..5767)
                     /locus_tag="PL075_01835"
     CDS             complement(5342..5767)
                     /locus_tag="PL075_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOD96963.1"
                     /translation="MVQKQQPKFKILPHTADVRLMIFGSSFEEIFQNALLGMAQILKP
                     QKASNVREIAYNIDINSLDPTALLIDFLSEVLYFSNVDHAVFENLVIERLTENSLKGK
                     IIGYEVEGFGEDIKAVTYHEAYVSQKASNSWEALVIFDI"
     gene            complement(5739..7127)
                     /locus_tag="PL075_01840"
     CDS             complement(5739..7127)
                     /locus_tag="PL075_01840"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOD96964.1"
                     /translation="MIKKLKKIIKKIVPETIINWYHYCLVFLAAFYYGFPSKDLIVIG
                     ITGTKGKTTVAELLNAFLESYGKKTAMINSLRFKINKQSWVNDLKMTMPGRFAIQKFL
                     ADCRQQKCYYVILEVTSEGIKQFRHKFINFDMAIFLNLQPEHLEAHGGSINKYCEAKE
                     KLFAALDQPRKNIPIVDKRGKMDCTVKKKMVVNLDDDYSNRFLKYKADEKIGFTLEKS
                     STKGVDKIFKPDSYKLSAEGIQFKLSNLDFFSPLKGKFNLYNLLAALSAAEGFCIPLS
                     IIQDAMSRFEGIPGRFEEITEGQKFKVFIDFAHTPDSLMAVYETIRTQFLPSPQSRLV
                     CVLSATGGGRDKWKRPKMGEVAAKYCDEIIITDEDPYDEDPKKIMENIAAGVKNKNKK
                     YQIIEDRRKAIAEALRLCQPEDCLIITGKGAERVMIIKDDKRIPWDDRAVAREELHKI
                     LQQDGSETTAKI"
     gene            complement(7128..7715)
                     /gene="ruvA"
                     /locus_tag="PL075_01845"
     CDS             complement(7128..7715)
                     /gene="ruvA"
                     /locus_tag="PL075_01845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOD96965.1"
                     /translation="MMYFIDGEVVRLAPKFIVLKSGDIAFKIFVHPQTLAQIGNQKEK
                     IRFFVYLNVKEDALELYGFLTQEELEFFELLNSVAGVGPKSALALLGFSEVKSLKAAI
                     LSNKIEVLTKAPGIGRKTAERIILELKSKISTEEGAIEELEADLELEDALLDLGYSKI
                     DIRRIIKQASNKQLPLNERLKNALKLLGEKKNHSD"
     gene            7926..8201
                     /gene="rpsT"
                     /locus_tag="PL075_01850"
     CDS             7926..8201
                     /gene="rpsT"
                     /locus_tag="PL075_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOD96966.1"
                     /translation="MPITQSAKKALRKSKKHYETNKKAEGVLKSEIKKFQKLLAGQKI
                     KEAEQQLSIVYKKLDKAAKSHLIKKNKAARLKSRLGKKLNVLLSKNA"
     gene            8222..8533
                     /locus_tag="PL075_01855"
     CDS             8222..8533
                     /locus_tag="PL075_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HOD96967.1"
                     /translation="MVLPTKKFQKKKESFICRHCGAIVEGNGYTDHCPHCLWSQHRDI
                     NPGDRKEKCHGLMKPVGVEIKKGYYLIYYQCQKCGYQFKVKAAKNDNFEEILKLSAFP
                     F"
     gene            complement(8583..8654)
                     /locus_tag="PL075_01860"
     tRNA            complement(8583..8654)
                     /locus_tag="PL075_01860"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8619..8621),aa:Met,seq:cat)
     gene            complement(8795..9211)
                     /locus_tag="PL075_01865"
     CDS             complement(8795..9211)
                     /locus_tag="PL075_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96968.1"
                     /translation="MQLQIQITSSVWRYLVYFWNIFLYVVIILDFISVNHYSDLLDLV
                     AVIYIAILSIYAGTKEFERWFSHYNAHHPGEVFIVSWTILLVILLVVPNVIQIDYHLP
                     EIIIYCYISVLTILALTRRSRSLYYAKKNKKDILEN"
     gene            9366..9441
                     /locus_tag="PL075_01870"
     tRNA            9366..9441
                     /locus_tag="PL075_01870"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9402..9404,aa:Asp,seq:gtc)
     gene            9460..10482
                     /gene="pheS"
                     /locus_tag="PL075_01875"
     CDS             9460..10482
                     /gene="pheS"
                     /locus_tag="PL075_01875"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOD96969.1"
                     /translation="MTIEEINQKVFEEIGKCKDLKTLEKIRIKYLGRKGELTNILKSL
                     KDLPIEQRKKIGPISNNLKKLIENALDEKRLYLISNHELEPLDLSLPGKKIPHGHLHP
                     FTTLENKIEEIFSALGFEIVESNEIETEYYNFDALNIPANHPARDMWDTFWIKNDFQS
                     KNKERFLLRTHTSPIQVHYMETHQPPFRIVSPGKVYRYEALDASHDIQFHQLECLMVD
                     ENITFANFKFIVLSFFQQLFSKKINVQFTPSYFPFVEPGVQINISCPRCNQKGCSLCK
                     NTGWLEIAGAGMVHPNVFKAVGYNPKMWRGFAFGIGLERIAMIKYNIPDIRLFNSADL
                     RFIKQF"
     gene            10488..12524
                     /gene="pheT"
                     /locus_tag="PL075_01880"
     CDS             10488..12524
                     /gene="pheT"
                     /locus_tag="PL075_01880"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOD96970.1"
                     /translation="MKFSYNWLKEYLPKLPAVQKLAENLTLKSFEVEKIEKNNSDIVM
                     DINLPPNRFSDSSGHLGLAKEISAILDTPLKTPVFHLKEDRRLKASNFLQIKVQNKND
                     CLRYSSRVIFDVKVKESPDWLKKRLESCGLQSINNIVDATNYVMLLTGQPLHAFDYDK
                     LMGEKIKTLIVRRGLSKEKIATLDDKEVELNDNILVIADLKNPLAIAGIKGGKIAEID
                     NNTKRIVLESANFDHALIRRVSQITKIVTDASLRFERILSPSLTIDALNYVAALIQKL
                     ANGKILNGIIDVFPTKISKKVLGFNFEQFYNFAGFTIDKKFIEKTFKRLDFKIVKKTN
                     NNLFLEIPPQRIDIERFEDLTEEILRLYDYNKAPAIAPRSLILPAPINEKALFRNKVK
                     SVLIGLGIDEIYAYSFVSKKDLDNFEIPEKNVIALENPISNDFAYLQPTLLINLIKAG
                     GSNLRFYDSVRIFEISKTFQKAPFRPYEEWRLAIVLSSKDEQSDPLFFELKGVIEQLT
                     EGLNCTDITFVDANTNWLAKNRAAKIIKDGEEIGYIGQINPRLSYLYNDDIPMVVAEL
                     DIEKLYQLSKEEYEFTPLPKYPAVIRDISILVNRDIRMSDILNIIYQSDPKIIFDVDL
                     FDIYEGKNLPENKKSLSFHLIFQAEDHTLTNDEVGKAIEKIIINLKNKFNAEIR"
     gene            12571..12643
                     /locus_tag="PL075_01885"
     tRNA            12571..12643
                     /locus_tag="PL075_01885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12604..12606,aa:Val,seq:tac)
     gene            12725..13039
                     /locus_tag="PL075_01890"
     CDS             12725..13039
                     /locus_tag="PL075_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOD96971.1"
                     /translation="MVERPEASGASGVQITMYFVYIIQSKKTKKYYIGTSNDPNSRIN
                     EHNRGATTYTKSGIPWILIKIMPCKNKTDALKLEKFIKKQKSRKFIEKIINEGMNFYL
                     FN"
     gene            13055..13369
                     /locus_tag="PL075_01895"
     CDS             13055..13369
                     /locus_tag="PL075_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOD96972.1"
                     /translation="MVERPEASGASGVQITMYFVYIIQSKKTKKYYIGTSNDPNSRIN
                     EHNRGATTYTKSGIPWILIKIMPCKNKTDALKLEKFIKKQKSRKFIEKIINEGMNFYL
                     FN"
     gene            13371..13442
                     /locus_tag="PL075_01900"
     tRNA            13371..13442
                     /locus_tag="PL075_01900"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13403..13405,aa:Phe,seq:gaa)
     gene            13483..13542
                     /locus_tag="PL075_01905"
     tRNA            13483..13542
                     /locus_tag="PL075_01905"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
BASE COUNT         4704 a         2323 c         2259 g         4268 t
ORIGIN      
        1 tagatgcaat taatattgat ctgaaatcga tgcaggaaaa attttatcaa aaaatttgtg
       61 gagcatatct agaacccgtg ctcaaaaata ttcaaaattt ttatgaaaaa ggcgtctggg
      121 ttgaattaac aacattgatt attcccaatg aaaatgatag cgaaaaggaa ttgaaacaaa
      181 ttgctaaatt tatagctgaa attgatcaga atatcccatg gcatatttcc cgattttttc
      241 cttcttatca aatggaaaat tatccaatga caccaatgaa cactttgaaa aaagcttatg
      301 aaatcggaaa agaaaatggc cttcattttg tctatgcggg aaatattatt gaagaatctt
      361 tggaaaatac ttattgtcca aagtgtggag ttttgctaat tgaacgtcaa ggttataata
      421 taaaaataaa agatgatttt agaaatggtc ggtgcttaaa ctgcaaagcc gctatcaagg
      481 gcgtttggaa ataataatat gaacattcga ccacctgttg ttgccggaca attttatcct
      541 gaagatcccc gcgaattgaa agaaacgatt caggaatttt tagatcaaca gccgaaagat
      601 ttgtttattc cccaaaaaat aagggcattg gttgtgccac atgctggcta tatttattct
      661 ggacccgttg cggccgcagc ctatcaaacg cttcggctgt ctcgttttga acctcaaaaa
      721 attgttttga ttgggccatc gcatcacttc cccattgctg actatatcac ttcttctttt
      781 gatcagtgga aaacgcctct aggcaaagtg cctcagataa aagtaactca aaatgaatgg
      841 actgtgtttg accttgcttt tatgcccgag cattctcttg aggtacaatt accatttttg
      901 caaaccattc tttcaaattt cgaaattttt cctattttaa ttaacaacat ttctcttagt
      961 gaaacattag ccagaaaaat aaaaagcttg ctcgatgaaa aaaccctcat catcgttagt
     1021 tctgatttaa gccactacaa cccattggca ataactcaaa aaatagataa acaaactcaa
     1081 gaaattattc ttcagctgaa aacaactgaa gccgaaaata ttgacgcttg cggagcggcg
     1141 ggaattttaa ctttaatgca tctagccaaa gaccttcatt ggcaaagaaa attaatttct
     1201 tatgccactt cttatgatag cagcaactat aaagatgaag ttgtcggcta tggtagtttt
     1261 cttttttatg aataactttt cattaactga aaaagataaa aaatttcttt tggaactggc
     1321 tcatcaaagc attcaatatt ttttgcaaca tcataaaatc tttgaaatcg atgaagaaga
     1381 attaaaaaat tattcacctg ccatcaagca aaaaagaggc tgttttgtca ccttaaccat
     1441 tgatgatgaa ctccgcggct gtattggctg ttttgaatca aataaacccc tttatcaaac
     1501 agtaatagaa atggctataa gtgctgcttt ttttgattat cgcttttctc ccttatctaa
     1561 agaggaatca aaacaaatca aaattgagat ttcgatttta actccgccac gagaacttaa
     1621 atataatgat gctgcagatc ttctgaaaaa attaaaaccg ctaaaacacg gtgttattat
     1681 tacaaaaggc aattatcaag ccacttattt accccaagtt tgggaacaaa ttcccggaaa
     1741 agaagaattc cttaattctc tctgtcttaa agccggattg ccatttgact gctggaaaaa
     1801 agaaaaatta aacgttaaaa tatacgaagt cgaaagtttt aaagaataaa ttataatcat
     1861 catggaaaca atcaatctaa atgattttaa aaagctggat cttcgcttag ctcaaattat
     1921 tgaagcagag cgagtgccgg atactaataa attgttaaaa ttaaaattag atcttggtga
     1981 tgaaactaga gaaattgttt cgggaattgg tatgcaatat catcctgaag acctcattgg
     2041 aaaacaaatt gttattgtag caaatttaga gcctaaagtt tttaaaggaa tagaaagtca
     2101 agggatgctg ctggctgtag taaataaaga tggtgatatt tctttattaa tacccgacaa
     2161 acaaatgacg ccaggcgatc gaattcaata acaacatctt gactttacta atagaaacaa
     2221 tataatcttt aatctgaact cgcattgtgt attttctaaa acaaaaggtc gtttttaaca
     2281 ttatcctatg agcgtgcttg ctcttgaaga tttacaaatc gttaatttag ccaaagaaga
     2341 tgatgaggac ttagatgaat tagaagagga tgtggaagaa gaaaaagaag aagagaaaga
     2401 tgatgatgat ttggatgact ttaacgaaga tgatattgat gaagatttat ttagtgacga
     2461 cgatttagaa gaagaaagtg aagaagaatt ataattaata atttaaaaag ccagccttaa
     2521 aaaggctggc ttttttttaa aaaaagttta ttataaaaat aatacaaaat ggttaaaaga
     2581 ttaacggaat caagttatat tcccaagaaa aaaaggcttc gcaaaactaa tacccgaaaa
     2641 gcattattta taggtgttgt ttttttattt gtagctattg taatagccgg tataggctac
     2701 attgtttatc tttattacac tctccccgat ccagctaatg ttggcgaaac gatgataaat
     2761 caatcaacta agatttatga tcgaacaggc gaagtcctgc tctatgaaat ccacggcgaa
     2821 gaaaaacgaa cagttattgc cctcagtgat attcctctcc atgttcaaaa agctacttta
     2881 gctattgaag atgccgaatt ttatcaacat cccgcattta attggaaatc actggtgaga
     2941 gcggttttta ttaatttaaa agagggtcgc tttgctcagg gtggctcaac gatcactcaa
     3001 caagttgtca aaaatattta tttaacaccc gaaaaaacta ttcaaagaaa aataaaagaa
     3061 attcttttag ctgtgaagtt agaagaacac tataccaaag atcaaatttt agaaatgtat
     3121 cttaatcaaa tcccctatgg ctctaatgcc tatggcattg aaactgctgc ccaaacttat
     3181 tttaacaaaa gcgcaaaaga tttgacgctg gctgaagcag ctattcttgc ggctctaccc
     3241 caagcgccaa gttattattc gccttatggc aataaccaaa aagaactttt tgatcgacaa
     3301 aaattagttt tgaaacgaat gttagattta ggatttatta ctcaagaaga atatgataca
     3361 gcggtaaatg aaaaaattaa attcgcttat cgaatagaaa aaattaacgc tccgcatttt
     3421 gttatgtttg taaagcaata tttggagaat aaatacggaa cggattatgt agaaaatgct
     3481 gggttaaaag ttataactac tcttgattgg gaccttcaac aaattgccga aagagttgtt
     3541 tatgaaggtg ctttaagaaa tgaaaagctt tataaaggaa aaaatgcggc tttaattgca
     3601 caagatccaa aaacaggcca aattttatcc atggtaggct ctcgtgatta ttttgattta
     3661 gatgttgatg gaaattttaa tgttattacc ggccatcgtc aaccaggttc atcttttaaa
     3721 ccttttgctt atttaactgc ctttgaaaaa ggattgacgc ctgaaacggt tgtttttgat
     3781 cttaaaactg aatttaattc taattgtcca gctacggctg atggcgatac ggatcgctat
     3841 ggtcttaatt gttatcatcc gcaaaatttt gatttgacgt ttaaggggcc tattgattta
     3901 agacatgctc tggctcaatc aattaatgtc ccggcggtga aagttcttta cctcgctggt
     3961 attaaagaca ctattgctac cgcacaaaaa atgggcgtca ctacacttac tgatatcaat
     4021 cgctatggcc tctctcttgt tttaggcggc ggagatatta aacccttgga ttttgcagaa
     4081 gcttattcag tttttgctca agatggcgta aaacatcagc aagcagctat tttaaaaatt
     4141 gaagatgcta aaggttctat tttagaagaa tacaaagatg taagcgaaca agttattgat
     4201 cctcaatata ttcgtctcat taatagcgtt ctttcggaca aagaagcacg tattggttta
     4261 tttaacccgg gcaatcaatt agaaattcct ggctatgagg tggcggccaa aactggcaca
     4321 actcaggatt atcgagatgc ttggactgtt ggttatacgc cttcgcttgt cgctattgtt
     4381 tgggctggaa ataatgattt ttcggcaatg caaaaaggtg gtggaagtat tttagcggct
     4441 gtacctattt ggcataattt tatgatcgaa gctctcaaaa aataccaacc agaaaccttc
     4501 ccccaacctg ccccgaccaa tccaaataaa cctgttctgc gcggagaata tattgttaat
     4561 ttcaaaattg gagacatcat ttatcctcaa atccataata ttctattttg ggttaataaa
     4621 aatgatattc tcggaccatc accattaaac ttcaatgatc ctcaggctaa taattgggaa
     4681 tatcccgtta ctcaatggat tcaaaataat ttacctaatc ctcaacaata caacatcgta
     4741 attccttata attatgctga acttcctcaa acaacactgc aaccaacatt ttcaatagaa
     4801 tttattaatc ctaaaaatgg agattatatt accggcggcg ttatctatgt tgtattaaac
     4861 attcaaacta ccaaccaaat aaaatcttta cggatctatt ttaatgataa tctggtacga
     4921 gaagatgtgg ctggcaatgg catttatcaa tttcaatttt cacccctcca aatcgaagat
     4981 caaaaccaac tcaaggttaa tatttgtgat gttctcaata gctgtactga aaaaagcatt
     5041 gttatcttta agcagcaaat ttaatcaaaa aataattttg attttattaa ttttaaagcc
     5101 tttttgagct aagaaaaacc ttaactcttc ctcaaaaaat tttaattcat ttgcctcaat
     5161 ggcatttttt actttgatag ttaaattgtg attaaaaaaa tttatttctt caacgctaat
     5221 ctgctggaga tgttctttaa gaaattcggc agtataattt attactgata tttcttttat
     5281 tttttgttta ataccgagct gatttaattt tttattaata atttttttaa tatcatccat
     5341 attagatatc aaaaattacg agtgcttccc atgaattgga tgctttctga cttacataag
     5401 cttcgtgata agtaactgcc ttaatatcct cgccaaaacc ctctacttca tatcctatta
     5461 ttttgccttt aagggagttc tctgttagtc tttctatgac taaattttca aaaacagcat
     5521 gatcaacatt cgaaaaatat aacacctcgg aaagaaaatc aatcaataaa gcggtgggat
     5581 ctagggaatt aatgtcaatg ttgtaagcaa tttctctaac gttacttgct ttttggggct
     5641 ttaaaatttg ggccattcct aataaagcat tctgaaaaat ttcttcaaaa gaagaaccaa
     5701 aaatcattaa ccgaacatct gctgtatgag gtaaaatttt aaattttggc tgttgtttct
     5761 gaaccatctt gttgcaaaat tttatgtaat tcttcacgag cgaccgctcg atcatcccac
     5821 ggaattcttt tatcgtcttt tataatcatt actctttccg ctcctttacc ggtgataatt
     5881 aaacaatctt ctggttggca taaacgaagc gcttcggcaa tcgctttgcg cctatcttct
     5941 ataatctgat attttttgtt tttatttttt acgcctgcag caatattttc cataattttt
     6001 ttcggatctt catcgtaagg gtcttcatcc gtaataataa tttcatcgca atatttagct
     6061 gctacctcgc ccatttttgg ccttttccac ttatcgcgac cgccacccgt cgcacttaaa
     6121 acacaaacta ggcggctttg agggctggga agaaattgag ttcttatcgt ttcataaacc
     6181 gccataagag agtctggcgt gtgagcaaaa tcaatgaata ccttaaattt ttgaccttcc
     6241 gtaatttctt caaatcgtcc gggaatgcct tcaaaccgac tcattgcatc ttgaataatg
     6301 gacaaaggaa tacaaaatcc ttctgctgca cttaaggccg ccagtaaatt gtaaagatta
     6361 aatttccctt ttagtggtga gaaaaaatca agatttgata atttgaattg aattccttca
     6421 gctgataatt tataactatc gggtttaaaa atcttatcaa cgccttttgt tgatgatttt
     6481 tcaagagtaa agcctatttt ttcatcagct ttatatttca aaaaacgatt gctataatca
     6541 tcatctaaat taactaccat tttcttttta accgtacaat ccattttgcc tcgtttatca
     6601 acaataggaa tatttttccg cggttgatct aatgccgcaa aaagtttttc tttagcctca
     6661 caatatttat tgatagaacc tccatgcgct tccaaatgtt cgggctgcaa atttaaaaat
     6721 atcgccatat caaaattaat aaatttatga cgaaattgtt taattccttc agaggttact
     6781 tctaaaatca catagtaaca tttttgttgt ctgcaatcag ctaaaaattt ctgaatggca
     6841 aaacggcctg gcatggtcat ttttaaatca ttaacccagc tttgcttatt aattttaaaa
     6901 cgcaatgagt taatcattgc tgttttttta ccataagatt ctaaaaaagc gttcaataat
     6961 tcagcaaccg tagtctttcc ttttgttcca gtaataccaa tcacaattaa gtctttcgaa
     7021 ggaaatccat aataaaaagc agctaaaaaa actaaacaat aatgatacca attgattatt
     7081 gtttcgggta caattttttt aataattttt tttagctttt taatcattta atcgctatga
     7141 ttcttttttt ctcctagcaa ttttaaggcg ttcttcaggc gctcgtttaa tggcaattgt
     7201 ttattagagg cttgtttaat aattcttctg atgtctattt ttgaataacc taaatcgagc
     7261 aaagcgtctt ctaattctaa atctgcttct aattcttcta tcgctccttc ttcggtgctg
     7321 atttttgatt ttaactcgag aatgatacgt tcagcagttt tgcgtccgat gccaggagct
     7381 tttgttagta cttctatttt attacttaaa atagccgcct taagactttt tacctcacta
     7441 aatcccaaaa gagcaagtgc tgattttggc ccaacgcctg ctactgaatt aagtagttca
     7501 aaaaattcta attcttcttg agtcaaaaaa ccgtaaagtt ctaaggcgtc ttctttaaca
     7561 tttaaataga cgaaaaatct gattttttct ttttgattac caatctgagc caatgtttga
     7621 ggatgaacaa atattttaaa agcaatatct ccgcttttta aaacaataaa tttgggcgcg
     7681 agtcgtacaa cctcgccatc aataaaatac atcatataat gtaattatat tatagaaatc
     7741 tcaaaatgca ataaaaaata aaagcggggt ggtgtggtgg cagcgatttt ctaaagaaaa
     7801 tcgctgccac cacacctttt tatattcatt ttatctaaac taatatcacc tatttttatt
     7861 atttattgtt tgactttttc ttttgttttt gtaaaataaa aacatatcaa ataacgctta
     7921 aaattatgcc tataactcaa tctgccaaaa aagccttaag aaaatccaaa aaacattacg
     7981 aaactaataa aaaagcggag ggtgttttga aatcagaaat caaaaaattt caaaaattgt
     8041 tagctggaca aaaaattaaa gaagctgagc agcaactttc catcgtatac aaaaagctag
     8101 ataaagccgc caaatctcat cttatcaaaa agaataaggc tgcgcgatta aaatcacgac
     8161 tgggtaaaaa attaaatgtg ctcttgtcaa aaaacgccta aagggcgttt ttaaatcgtt
     8221 tatggttttg ccaacaaaaa aatttcaaaa aaagaaagaa agttttatct gccgccactg
     8281 cggtgcaatt gtagaaggca acggctatac tgaccattgc cctcattgcc tttggtcaca
     8341 acatagagac attaatcccg gagacagaaa agaaaagtgt catggattaa tgaagcctgt
     8401 gggcgttgaa ataaaaaaag gatattatct tatctattat caatgccaga aatgcggcta
     8461 tcaatttaaa gttaaagctg ctaaaaatga caattttgaa gaaatattaa agttgagcgc
     8521 tttcccattt taaataaaat gggaaataaa attgttccgg cttcagaaaa atatactgaa
     8581 tgtgccgatg ggtggaatcg aaccaccgtc tgagggttat gaatcctccg ctctaaccat
     8641 tgagctacat cggcaaaaaa atcggaacaa ggagaaagtg ttgcgggacc aggatttgca
     8701 cctgggtctc gaggttatgg gcctcgcgag atactacttc tccatcccgc tgttctctat
     8761 tttataagtt tttcaatttt tttcaagaga aagcttaatt ttcaagaata tcttttttat
     8821 tcttttttgc ataatataga ctgcgcgaac gccgtgttaa agctagaatt gttaaaacgc
     8881 taatataaca atagatgatg atttcgggca gatgataatc tatttgaatg acgtttggca
     8941 caacaagcaa aataacaaga agtattgtcc aactgacaat aaacacttca ccaggatgat
     9001 gagcgttata atgggaaaac catctttcaa attcttttgt accagcataa atagacaata
     9061 tagcaatata aatcactgcc actaaatcta ataggtctga gtaatgattg acggaaataa
     9121 aatctaaaat aataaccaca tataaaaaaa tattccaaaa ataaaccaga taacgccata
     9181 ccgaagaagt gatttgaatt tgtaattgca tattattagt tattataaca aatgctcgtt
     9241 aaatttcaaa agatttcaat caaaaggctt caatcaaaat ttaaaacttt aaaaaataaa
     9301 aaacgcgacc attcatttta ttgcattttt tgataaaatc agataaaatg ttcttatcca
     9361 aaaacgggcc ggtagctcag cttggtttag agcgtctgcc tgtcacgcag aaggtcgcgg
     9421 gttcaagtcc cgtccggccc gccctaaatt ttaaatacta tgaccattga agaaattaat
     9481 caaaaagttt ttgaagaaat tggcaagtgt aaagatctga aaacgctaga aaaaatacga
     9541 attaaatacc ttggccgaaa gggagaatta actaatatct taaaatctct aaaagactta
     9601 cccattgaac agagaaaaaa aattggcccc atctccaata atttaaaaaa actcattgaa
     9661 aatgctttag atgaaaaacg actttatctc atcagtaatc acgaattaga gcctcttgat
     9721 ctaagcctgc cgggcaaaaa aattccacat ggtcaccttc acccctttac aacattagaa
     9781 aataaaatag aagaaatttt tagcgcttta ggttttgaaa tagtagaaag taatgaaatc
     9841 gagacagaat actataattt tgatgctttg aatattccag ccaaccaccc ggctcgcgat
     9901 atgtgggata ccttttggat taaaaatgac ttccaaagta aaaataaaga aagattttta
     9961 ttaagaacgc atacttcacc tatccaagtg cactatatgg aaacgcatca gccacctttt
    10021 cgtattgttt caccaggaaa agtttatcgt tatgaagctc ttgatgcttc tcatgatatt
    10081 caatttcacc aattagaatg tttaatggtt gacgaaaata ttacttttgc taatttcaaa
    10141 tttatcgtcc tatcattttt ccaacagctt ttctccaaaa aaattaatgt tcaattcacg
    10201 ccgagttatt ttccctttgt tgaaccgggc gtccaaatca atatttcttg cccacggtgt
    10261 aatcaaaaag gctgctcttt atgtaaaaat accggctggc tcgaaattgc cggcgcggga
    10321 atggtacatc ctaacgtatt taaggctgtg ggttataatc ctaaaatgtg gagaggattt
    10381 gcttttggaa taggactcga acgtatcgcg atgataaagt ataatattcc cgatattcgc
    10441 ctttttaatt cggccgatct aagatttatt aaacaatttt aggtggtatg aaattttcat
    10501 ataattggct taaggaatat ttaccaaaat taccagccgt tcagaaacta gcggaaaatt
    10561 taacattaaa aagttttgaa gttgaaaaga tagaaaaaaa taatagcgat atagtaatgg
    10621 acatcaattt gccacccaat cgtttttcag atagtagtgg tcatttggga ttggccaaag
    10681 aaatatcagc gatccttgat actcctctta aaacgccggt atttcatttg aaagaggaca
    10741 gaaggcttaa ggcaagtaat tttcttcaaa ttaaagttca aaataaaaat gactgcttgc
    10801 gttatagtag ccgcgttatt tttgacgtga aagttaaaga atcgcccgat tggttgaaaa
    10861 aaagattaga aagttgcggc cttcaatcta ttaataatat tgttgatgct actaattatg
    10921 tgatgcttct aacgggtcaa cctcttcatg catttgatta cgataaattg atgggagaaa
    10981 aaattaaaac cctcatagtt agaagaggtc taagtaaaga gaaaatagca acgctcgatg
    11041 ataaagaagt agagctaaat gataatattt tggttattgc tgatcttaaa aatcctcttg
    11101 ccattgctgg aattaaagga ggaaaaatag cagaaatcga taacaacaca aaacgcattg
    11161 ttttagaaag cgctaatttt gaccatgcgc tgattcgccg agtctcacaa ataacaaaga
    11221 ttgtaaccga cgcttctttg agattcgaac gcattctttc accttcatta actattgacg
    11281 ccttaaatta tgtagctgct ttaattcaaa aattagctaa tggcaaaatc ttaaatggaa
    11341 ttattgatgt tttcccaact aaaatttcca agaaagtcct cggttttaac tttgaacaat
    11401 tttataattt tgctggattt actattgata aaaagtttat cgaaaaaact tttaaacgat
    11461 tagatttcaa aattgtaaaa aaaacaaaca acaatctgtt tttggaaatt cctcctcaac
    11521 gaattgatat tgaaaggttt gaagatttaa ccgaagaaat tcttcgcctt tatgattata
    11581 ataaagcgcc ggctattgca cctcgctctc ttatccttcc cgctcccatt aatgaaaaag
    11641 ctcttttccg caataaagta aaaagtgttc tgattggttt agggatagat gaaatctatg
    11701 cttattcttt tgtttcaaaa aaagatttag ataatttcga aataccagaa aaaaatgtga
    11761 ttgcattaga aaacccgatt agcaatgact ttgcctatct tcagccaacg cttttaatta
    11821 atttaataaa agcgggcgga tctaatttgc gtttttatga tagtgtgaga atttttgaaa
    11881 ttagtaaaac attccaaaaa gcaccatttc gaccctatga agaatggcgt ttagccattg
    11941 tattatcaag taaagacgaa cagagtgatc ctctattttt cgaattaaaa ggcgtgatcg
    12001 aacaattaac cgaagggtta aattgtactg acatcacttt tgtagatgct aacactaatt
    12061 ggttggccaa aaatcgagca gctaagatta taaaagacgg agaagaaatt ggttacattg
    12121 gccaaatcaa tcctcgcctc tcttatttat ataacgacga tatccctatg gtggtagcag
    12181 aattagatat agaaaaatta tatcaattat ccaaagaaga atacgaattt acaccattgc
    12241 ctaaatatcc tgctgttatt cgtgatattt ctattttagt taatcgtgat attcgaatgt
    12301 ctgatatttt aaatattatt tatcaaagtg acccaaaaat tatttttgat gttgatcttt
    12361 ttgatattta tgaagggaaa aatttaccag aaaataaaaa aagcctaagt tttcatttaa
    12421 tctttcaagc agaagatcat actctaacta atgatgaggt ggggaaagca atagaaaaaa
    12481 tcatcatcaa cttaaaaaat aaattcaatg ctgaaatcag ataagtagag ggtggcttga
    12541 aaagcaaatt ttctctaacc gatgtcaaaa gcgcgcttag ctcagttggc agagcgtctc
    12601 tcttacacag agaaggtcgg gggttcgaat ccctcagcgc gcacgttaaa ttcattgctc
    12661 gttgaaaatt ttaaaaaaat acataaaata aatggagctg tttaattaat gcctcggtag
    12721 ctcagtggta gagcgtcccg aagcatcggg agcgtcgggg gttcaaatta caatgtattt
    12781 tgtttatatt atccaaagca aaaaaacaaa gaaatattat attggcacat ctaacgatcc
    12841 gaactcaaga attaatgaac acaatagagg agcaacgact tatacaaaaa gcgggattcc
    12901 ttggatttta ataaaaataa tgccttgcaa aaacaaaacc gatgctctaa aattagaaaa
    12961 atttattaaa aaacaaaaaa gtagaaagtt tattgaaaaa ataataaacg aaggaatgaa
    13021 tttttatctt tttaattaat gcctcggtag ctcagtggta gagcgtcccg aagcatcggg
    13081 agcgtcgggg gttcaaatta caatgtattt tgtttatatt atccaaagca aaaaaacaaa
    13141 gaaatattat attggcacat ctaacgatcc gaactcaaga attaatgaac acaatagagg
    13201 agcaacgact tatacaaaaa gcgggattcc ttggatttta ataaaaataa tgccttgcaa
    13261 aaacaaaacc gatgctctaa aattagaaaa atttattaaa aaacaaaaaa gtagaaagtt
    13321 tattgaaaaa ataataaacg aaggaatgaa tttttatctt tttaattaat gcctcggtag
    13381 ctcagtggta gagcgagtgc ctgaagagca ctgcgtcggg ggttcgattc cctcccgagg
    13441 cacattaatt aattaattgc ctctttaata aaacaataaa acggcttagt ggtagagcgt
    13501 cccgaagcat cgggagcgtc gggggttcga ttccctcccg aggcacatta atta
//
LOCUS       DAOSMC010000013        10551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_7164_length_10551_cov_21.537348, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000013 DAOSMC010000000
VERSION     DAOSMC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_10551_cov_21.537348"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1379)
                     /locus_tag="PL075_01910"
     CDS             complement(144..1379)
                     /locus_tag="PL075_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOD96973.1"
                     /translation="MNLTRIFYKNWKPVFCFFGGFNEIIASLVFINTDNKKLKIKKYY
                     QIINNDEDGFGEKDIIDALAHLIKKISKKIPLLLCFEQPFAKSTKARFSVVRDNPLSP
                     IDKEELENAILNLAWRLYDKERIFVSQKLKISEWDVVLADSKISDPRIDDRKVLNPIG
                     FSGKSLSFCLENTYLNYLFWENVKDILERWGGELIFCAEKNLIFDKISSLIEGKERIA
                     FVEITSRSTSAAVLGNYLNSYRSFDWGSINLIKSLSQRLGISLEQAEELKTIYSQGAL
                     SEAAHEWFKKLFEKELRLLAQGINLAFKDMGLNEKISHIYLTGDLAELPDLIPFLENQ
                     KWNAAIFVDPPKIQFYDALQLMKDLNLELIDFNEKKLSINFVINLAAIFLGDNKYQAM
                     NKILKRRIKWLNQNLLKKR"
     gene            complement(1403..3178)
                     /locus_tag="PL075_01915"
     CDS             complement(1403..3178)
                     /locus_tag="PL075_01915"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOD96974.1"
                     /translation="MTANELREKYLNFFEERGHKRLPSAPLIPENDPTVLFTTAGMHP
                     LIPFLMGEKHPAGQRLCDVQKCLRTDDIDEVGDIAHLTFFEMLGNWSLGDYFKKEAIE
                     WSYEFLTSLKWLGLEKEKLAVSCFGGDDVVPKDEESAQLWQNVGIPKERIAFLGRKEN
                     WWGPIGETGPCGPDTEMFYWVGRDGAPKIFNPQDPNWVEIWNDVFMTYNKTKEGKYEI
                     LQQKNVDTGMGLERTLAVINHLSSVFETELFVPIIKKIEEISNKKYGDFSKEFRIIAD
                     HLKASIFLLAERLEPSNVERGYILRRLIRRAIRFGRLLDIKDFFAFKLIEPVLAIYGE
                     TYPELNENKDFIEEQLVREEEKFSKTLSKGLSLFEKLAQNKKQISSKEAFDLYQSYGF
                     PLELIKELAKEKNIEVDVIGFEEEFKKHQELSRTASAGMFKGGLASHSEKAIKYHTAT
                     HLLHKALQEVLGEEVKQEGSNINEERLRFDFSCSKKLTPEQLKKVEEIVNQKIKENLP
                     VIMKEMKLEEAKKIGAIGLFNEKYGDRVKVYFIGRLEDGLENAYSKEICGGPHVTNTN
                     VLGHFKIIKEEAVSQGVRRIKAILE"
     gene            complement(3335..3703)
                     /locus_tag="PL075_01920"
     CDS             complement(3335..3703)
                     /locus_tag="PL075_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96975.1"
                     /translation="MKKDGNGKRMKQLAWQLVKLREEKNRIEKKERKVADTLIKLMEK
                     YHKNEIDIEEKNKTVKKIQSLILEIDPLRLYRLMPMKQFCQVVKVQPQPVIKMLGLER
                     VLKIAERKETKPYLRVEEKH"
     gene            complement(3776..5101)
                     /locus_tag="PL075_01925"
     CDS             complement(3776..5101)
                     /locus_tag="PL075_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOD96976.1"
                     /translation="MAWGEKMRKIILAIGDLVCFYFSLFLMATIRHLNADYFFIHLKL
                     FSIIFVVWLAVFYIGHLYELENFKDRKKLFNLICRLQLINIVLAIIIFYLYPVITPKT
                     NLALIIIFSSFFILLWRYLVTPIFLKKIAYRALLLGNSKEIEEINKLLSESSVMGYQL
                     VKWIDDPFRFQENELKNLVEKEKIRIIVIDDLLRDGANKGSLQKFVIEEMFQQKEIYD
                     TIEFYELVFKRIPLSVVDENWFLNYLKVYRQNHFFSTKRIFDFVAALTIFLISLIFWP
                     FIVLAIKIDSPGPIFYSSWRVGQNGRLFKIYKFRSMYALPEDENWPRWANQNDSRITK
                     VGKFLRKTHLDELPQLINILKGDMSFVGPRPIEKKLFDLCIHEKPIYYLRNIVKPGLV
                     GWAQLNYSYAASLEDELKKFEYDLYYLRNRSFWFDIAIIARTIKILLSF"
     gene            complement(5113..6039)
                     /gene="secF"
                     /locus_tag="PL075_01930"
     CDS             complement(5113..6039)
                     /gene="secF"
                     /locus_tag="PL075_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOD96977.1"
                     /translation="MIDFIKVRKITYWISGALVLIAWISLIIFGLKPGMDFTGGSALE
                     LKLNNVNIDHQFITDVYKQFGYQNISIAPSGDDGLTIRSTDITEENHQKIITQIKEKY
                     NEVEEMRFESFGPVIGRELRNKSIQGIILVVICIVLYLSYAFRQVSRPMASWHYGVTA
                     ILSLIHDISIPTGLFAILGHVHGVEIDSNFIVALLVILGFSVHDTIVVFDRIREKLRT
                     HPGLDFGQVMNESINETLRRSIFTSLTLVLVLLALLIFGPASLFYFTLTLLIGTFFGT
                     YSSIFIASPLLYDWYLFLQKRKNKKQGEIKKR"
     gene            complement(6041..7336)
                     /gene="secD"
                     /locus_tag="PL075_01935"
     CDS             complement(6041..7336)
                     /gene="secD"
                     /locus_tag="PL075_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOD96978.1"
                     /translation="MKENSKNLLIVIIILIVAIIAGLFAYGELDFLPNWFKKPYRLGL
                     DLSGGTRLIYEADLSQVAEADRDSAMNGLRDVIERRVNLFGVSEPRVEVVQVGDSYRL
                     NVELAGVKDINQAIQMIGETPYLEFREQRPQEETEKILEEQKNNNPEYQFVDPYFVPS
                     QPPLTGKYLKTARLDFDPRTYEPVVSLEFNDEGAKIFAELTQKNVGKVLAIYLDGQPI
                     SLPVVQEEITEGKAQITGKFTADEAKKLVENLNAGALPVPIKLISQQSIGALQGEFFL
                     KLSIKAGVIGLIAVIIFMLVMYGKLGIFSSLALIIYTILNLAIFKLIPVTLSLSGIAG
                     FILSIGMAVDANILIFERYREEKKIGLENKRALFESFKRAWPSIRDSNISTIITCLVL
                     YNFTSSMVKGFALTLLIGVVTSMFTAITVTRSLLKAFIK"
     gene            complement(7505..8155)
                     /gene="thyX"
                     /locus_tag="PL075_01940"
     CDS             complement(7505..8155)
                     /gene="thyX"
                     /locus_tag="PL075_01940"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOD96979.1"
                     /translation="MKVISPSFKVVTELNREEILRKIELAGRISHKSEEKITEDSASK
                     FVKHLLKIGHESVLEHVNLSVIIVCDRGVSHELVRHRLASYTQESTRYCDYTKKGEIV
                     VIKPVFLTEGTEKYRVWKTTMELIEKSYFHLRELGLKPEEARAVLPNSLKTEIFVTAN
                     IREWRHIFELRCNAAAHSQMREIMIPILELFQQKLSPLFDDFSVDLEKNTAIRASF"
     gene            complement(8207..9070)
                     /locus_tag="PL075_01945"
     CDS             complement(8207..9070)
                     /locus_tag="PL075_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOD96980.1"
                     /translation="MEKIKEELGPIKEAVYLIEVEKIKPNPYQPRKRFDEESLKELAD
                     SIREFGILQPLIVSRVEKETPYGRGVEYQLIAGERRLRAAQMLGLERVPAIIRPSFEE
                     RQKLEAAIIENIQRADLNPLESARGFAKLAEEFGLSQREIAERIGKSRAFVANALRLL
                     ELPSEAQRALEEGKITESHARLLLTITNPEKQRALLGEILSRKLTVRETEILTRRILE
                     IPLGVFSEQKQGVQVSDLGDALEKEIEKKLEAIFGTKVEVKKRGNKGKITINFFSDEE
                     LEEILKKLVKE"
     gene            complement(9200..10114)
                     /locus_tag="PL075_01950"
                     /pseudo
     CDS             complement(9200..10114)
                     /locus_tag="PL075_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            10256..10340
                     /locus_tag="PL075_01955"
     tRNA            10256..10340
                     /locus_tag="PL075_01955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10290..10292,aa:Leu,seq:taa)
BASE COUNT         3430 a         2082 c         1587 g         3452 t
ORIGIN      
        1 ggaagttcaa aatagattcg ctaattctag cattttcagg aacgatcaaa ataaccgcct
       61 cattagagga attaataatt ttttccacaa catctgagac agaatcgttt aattcaacat
      121 aaatattttc tgctttcatt gtattaacgt tttttaagca aattttgatt aagccatttg
      181 atacgacgtt ttaaaatttt attcattgct tgatatttgt tatctcctaa aaaaatggcc
      241 gctaaattaa taacaaaatt aattgacaac tttttttcat taaaatctat cagttcaaga
      301 tttaaatctt tcattagttg taaggcatca taaaattgaa ttttcggagg atccacaaaa
      361 attgccgcat tccatttttg attttctaaa aatggaataa gatccggaag ctctgctaaa
      421 tctcctgtta aataaatatg gcttatcttt tcgtttaatc ccatgtcttt aaatgccaaa
      481 ttaatacctt gagctaaaag tcttaactct ttttcaaata attttttaaa ccattcgtgc
      541 gcagcctcac ttaatgctcc ttgactataa atagtcttca actcttctgc ctgttctaac
      601 gagatgccca atcgttgcga cagtgacttt attaaattta ttgaccccca atcaaacgat
      661 cgataagaat ttaaataatt gcctaagaca gctgcgctgg ttgatcgaga agtaatttca
      721 acaaaagcaa tccgctcttt cccttctatt aaagatgaaa ttttatcaaa aatcaaattt
      781 ttttcagcac aaaaaattaa ctccccaccc catcgctcta aaatatcctt aacattttcc
      841 caaaataaat aatttaaata ggtattctct aaacaaaaag ataaggattt accgctaaag
      901 ccgatggggt ttaatacttt tcggtcgtca attcgcggat cgctaatttt tgaatcagcc
      961 aatacaacat cccattcgga aatttttaat ttttgactaa caaaaatgcg ttctttatca
     1021 taaagtcgcc atgctaaatt taaaatagca ttttcaagtt cttctttatc aataggcgac
     1081 agtggattat ctctaacgac ggaaaaacgc gctttcgtag atttagcaaa aggttgttca
     1141 aaacataaaa gcagaggaat ttttttggaa atttttttaa tcaaatgggc aagtgcgtca
     1201 ataatatcct tttcaccaaa tccatcttca tcattattaa ttatttgata atatttttta
     1261 atttttaatt ttttattatc agtattaata aaaacgaggc tggcaataat ttcgttaaaa
     1321 ccgccaaaaa aacaaaatac tggtttccaa tttttataaa aaatgcgggt taaattcatg
     1381 tgtccgtttt attataacat ttttattcca agatcgcctt gattcgtctt actccttgag
     1441 aaactgcttc ttctttaata attttgaaat gacccaatac atttgtattg gtcacatgcg
     1501 gaccgccaca aatttctttg gaataagcat tttctaatcc atcttcgagc cgaccaataa
     1561 aataaacctt aacacggtcg ccatattttt cattaaaaag gccaatggct ccaatttttt
     1621 ttgcttcctc caatttcatt tctttcataa taactggcag attttcttta attttttgat
     1681 taacaatttc ttctactttt tttaattgtt cgggtgttag tttttttgaa caagagaaat
     1741 caaaacgcaa acgttcctca ttaatattac tgccttcctg tttgacttct tctcctaata
     1801 cttcttgcaa agctttgtga agtaaatggg tagctgtgtg atacttaatc gccttttcag
     1861 aatgactcgc caaccctcct ttaaacatac cagccgaggc cgtacgcgaa agttcttgat
     1921 gttttttaaa ttcctcttca aatcctataa catcaacttc aatatttttt tcttttgcta
     1981 attctttaat taattccaat ggaaatccat agctttgata taaatcaaaa gcttctttgc
     2041 tagaaatctg ctttttattc tgggccaact tttcaaacag agataagcct ttagaaagcg
     2101 ttttgctaaa tttttcttct tctcgcacta attgctcttc aataaaatct ttattttcgt
     2161 ttaattcagg ataggtttct ccataaatgg ccaaaacagg ttcaattaac ttaaaggcaa
     2221 aaaaatcttt aatgtccaac aatcggccga aacgtattgc tcgtctaatg agacgtcgca
     2281 aaatataacc acgttccaca ttggaaggtt ctaatcgttc ggctaaaaga aaaatgcttg
     2341 cttttaaatg atcggcgata attcgaaatt cttttgaaaa atctccgtat tttttattgc
     2401 taatttcttc aattttttta atgatcggaa caaaaagttc tgtttcaaag accgaagata
     2461 aatgattgat aacagccagg gtacgctcta atcccattcc cgtatcaaca tttttctgtt
     2521 gaagtatttc atatttacct tctttggttt tgttgtatgt cataaaaaca tcattccaaa
     2581 tttctaccca attaggatct tggggattaa aaatttttgg tgctccatct ctaccaaccc
     2641 aatagaacat ttccgtatct ggtccacaag gaccagtttc accaatgggt ccccaccaat
     2701 tctcttttct acctaaaaat gcaattcgct ctttaggaat accgacattt tgccacaatt
     2761 gagctgattc ttcatctttc gggacaacat catctccgcc aaaacaactg actgctaatt
     2821 tttccttttc taagcccaac cacttgagag aagttaaaaa ttcgtagctc cattcaattg
     2881 cttctttttt aaaataatct cccaatgacc aattgccgag catttcaaaa aatgttaagt
     2941 gggcaatatc gcctacttcg tcaatgtcat cggttctcaa acatttttga acatcacata
     3001 aacgctgacc agccggatgt ttttcgccca ttaaaaatgg aattaatgga tgcatgccgg
     3061 cggtcgtaaa taaaaccgtc ggatcatttt cgggaattaa aggagccgaa ggcaaccttt
     3121 tatgccctcg ctcttcaaaa aaatttaaat atttttctcg caattcgttt gctgtcatta
     3181 ttattattgt aatcaatttt tgagaaaaat aaaacgatag gaaatgtaaa tctcaaatta
     3241 taaaattctt aaaagtttcg cttcataaca cacaagaaaa attaataaaa agtccgctta
     3301 aaagcggact gattttttta atgcttctct ttttttaatg cttctcttca actcgcaaat
     3361 acggcttagt ttcttttctt tcggctattt ttagaactct ttccagtcct aacattttaa
     3421 taactggctg gggttgcact ttcaccactt ggcaaaattg cttcatcggc attaaccggt
     3481 aaagacgcaa aggatcaatt tctaaaatta gcgattgaat tttcttaacg gttttatttt
     3541 tttcttcaat atcaatttca ttcttgtgat acttctccat caatttaatc agggtgtctg
     3601 ctacttttct ttcttttttt tcaatcctat ttttctcttc tctcaatttc accagttgcc
     3661 aagctaattg cttcattctt ttgccattac catccttttt catttctttt cctcctttat
     3721 aaaatttttg attgctaatt ttagcttaaa gctttaataa attttgtcaa tcaaattaaa
     3781 aacttaataa aattttgatc gtccgcgcaa taattgcaat atcaaaccaa aaagagcgat
     3841 tgcgtaaata ataaaggtcg tattcaaatt ttttgagctc gtcttccaaa gaagctgcat
     3901 aagaataatt taattgagcc caacccacca atcctggttt aacaatattt ctaagataat
     3961 aaatgggttt ttcgtgaata cacaaatcaa aaagcttttt ttctattggc cgtggtccaa
     4021 caaaagacat atcacccttc aaaatattaa ttaattgagg caattcatcg agatgtgttt
     4081 ttcttaaaaa tttgcccacc ttagtaatgc gggaatcgtt ctgattagcc caacgtggcc
     4141 aattttcatc ttctggcaag gcgtacattg aacgaaactt gtaaatttta aataatcgcc
     4201 cattttgacc cacacgccac gatgaataga aaatagggcc cggtgaatca atttttatcg
     4261 ctaaaacaat aaatggccaa aaaattaaac taatcaaaaa aatggttagc gctgctacaa
     4321 aatcaaaaat tcgttttgtc gaaaaaaaat gattttgacg ataaactttt aaataattta
     4381 aaaaccaatt ttcatcaact accgaaagag gaatgcgttt aaaaaccaat tcgtaaaatt
     4441 ctattgtgtc ataaatttct ttttgttgga acatctcttc aataacaaat ttttgaagag
     4501 atcctttatt agcgccatcc ctcaaaagat cgtcaataac aataatcctt attttttctt
     4561 tttcgactag gttttttagc tcgttttctt gaaaacggaa gggatcgtca atccatttaa
     4621 ctaattgata tcccattact gaagactccg aaagtagttt gtttatttct tctatttctt
     4681 tactattgcc caacagcaaa gcgcgatagg caattttttt gagaaaaatc ggggttacca
     4741 agtaacgcca taataaaata aaaaatgagg aaaaaataat aataagtgct aaatttgttt
     4801 ttggagtaat aacaggataa agataaaaaa tgattatggc taaaacaata ttgataagtt
     4861 gtaaacgaca aatcaaattg aaaagttttt tgcgatcttt aaaattttcc agttcataaa
     4921 gatggccgat ataaaaaacc gctaaccaaa ctacaaaaat aattgaaaag agttttaagt
     4981 ggataaaaaa atagtcggca ttgagatgac gaatcgtagc cattaaaaaa agggaaaaat
     5041 aaaaacagac gaggtcgccg attgccaaaa taatttttct cattttttcc ccccaagcca
     5101 taaatttttc aattatcgct tttttatttc cccttgcttt ttattttttc tcttttgtaa
     5161 aaacaagtac caatcgtata aaagtggact ggcaataaaa atggaggaat aagttccaaa
     5221 aaaagtacca atgagcaaag tcaaagtgaa atagaaaaga ctagctggac caaaaattaa
     5281 taatgctaaa agcaccagaa ccagagttaa agaagtgaaa atggaacggc gcaaggtttc
     5341 gttaatgctt tcattcatca cctgcccgaa atccagacct ggatgtgtac gcaatttttc
     5401 tcgaatccga tcaaaaacaa caattgtatc atgaacagaa aaaccaagaa taaccaataa
     5461 cgctacaata aagttagaat cgatttccac cccatgaaca tgacctaaaa tagcaaacag
     5521 accggtagga atgctaatat cgtgaatcaa ggataaaata gctgtaacgc catagtgcca
     5581 agaagccatt ggtcgcgaga cttgacgaaa ggcataactc agataaagca cgatacaaat
     5641 tacaactaaa ataattcctt gaatagattt attgcgtaat tctctaccaa taaccggtcc
     5701 aaaagattca aaacgcattt cttctacctc gttatatttt tctttaattt gagtaataat
     5761 tttttggtga ttctcttcag taatatcagt agagcggata gtcaaaccat cgtcacctga
     5821 gggcgcaata gaaatatttt gataaccaaa ctgtttataa acatcggtaa taaattgatg
     5881 gtcgatgttg acattattta attttaactc taaagcagag ccgccggtaa aatccatgcc
     5941 gggctttaag ccaaaaataa tcaaagaaat ccaagcaata agcactaatg ccccagaaat
     6001 ccaataagta atttttctta ctttaataaa atcaatcata ttatttgatg aatgctttta
     6061 ataatgaccg cgttaccgta atagccgtaa acatactcgt caccacacca attaataagg
     6121 taagggcaaa tccctttacc atagaactcg tgaaattata cagcacaagg caagtgataa
     6181 tagtagaaat attggaatcg cgaatagacg gccaagcccg cttgaaactt tcaaacaaag
     6241 cccgcttatt ttctaaacca atttttttct cttcccgata acgttcaaag attaaaatat
     6301 ttgcatcaac cgccatacca atagataaaa tgaagccggc aataccagaa agactcagtg
     6361 tcactggaat taatttgaaa atagccaagt ttaaaatagt ataaataatt aaagctaagg
     6421 aggaaaaaat tcctaatttg ccatacatta ccaacataaa gataataacg gcaatcaacc
     6481 caatcactcc ggctttaata ctcaatttta agaaaaattc tccttgaaga gcgccaattg
     6541 attgttgcga aattaattta atgggaaccg gtaaagcgcc cgcatttaag ttttccacta
     6601 atttttttgc ttcatcagct gtaaatttcc ctgtaatttg agctttgccc tcggtaattt
     6661 cttcctgaac taccggcaaa gaaatcggct gaccatcaag ataaatagct aaaactttgc
     6721 ctacgttttt ttgcgttaat tccgcaaaaa ttttagcgcc ttcgtcatta aattccaaac
     6781 taacaactgg ctcataggtg cgcggatcaa aatctaaacg cgctgttttc aaatattttc
     6841 ccgtcagagg cggctgagaa ggaacaaaat aaggatcaac gaattgatat tcagggttat
     6901 tatttttctg ttcttctaaa attttttctg tttcttcctg gggtcgttgt tcgcgaaatt
     6961 ccaaatacgg agtttcgcca atcatttgaa tggcttgatt aatatccttt acgccagcta
     7021 gttcaacatt caaacgataa ctatctccca cttgaactac ttccacacgc ggctcgctaa
     7081 cgccaaaaag attgactcgg cgttcgatga catcacgcaa accattcatt gccgaatcac
     7141 gatctgcttc agccacctga cttaaatcag cttcataaat taaacgcgtt ccgccactta
     7201 aatctaaacc gagccgataa ggttttttaa accagttcgg taaaaaatca agttcgccat
     7261 aagcaaaaag gccggcgata atcgctacaa ttaaaataat aataacaatc aataaatttt
     7321 tagaattttc tttcatagcg aaattatttt tattgtaatc caaaattaaa tatttgcaag
     7381 aaaaaaataa acataacaat taaaaatcta gaactctctg aggcggcaaa atttttctat
     7441 agaaaaattt tgccgcctct tcaatgttta tttttacttt ttacgtttaa aataaaagag
     7501 cgtcctaaaa ggacgctcta atggcagtat ttttttctaa atcaacacta aaatcatcaa
     7561 aaagtgggga aagtttttgc tgaaataatt ctaatattgg aatcataatc tctctcattt
     7621 gtgaatgtgc agcggcatta caacgcaatt caaaaatatg ccgccattcg cgaatattcg
     7681 ccgtcacaaa aatttctgtt ttcaacgaat tcggcaacac cgcccgtgct tcttctggct
     7741 ttaaaccaag ctctcttaaa tgaaaatagg atttttcaat caactccatt gttgttttcc
     7801 acacgcgata tttttcagtt ccttcagtta aaaataccgg tttaattacc actatttcac
     7861 ctttcttggt gtaatcgcaa tagcgagtgg attcctgagt ataactcgcc aaacgatggc
     7921 gaactaattc atgagaaaca ccgcgatcac aaacaataat aactgaaagg ttgacgtgtt
     7981 ccaatacgct ttcatgaccg attttcaaca aatgctttac aaatttagat gctgaatcct
     8041 cggtaatttt ttcttcactt ttgtgggaaa ttcgcccagc caactcaatt tttcttaaaa
     8101 tttcttctcg attcaactcg gtcacaactt taaaagacgg actaatgact ttcatcatcg
     8161 cctccttata aaaaattaaa tagctgattc tagtttaagc taaattttat tctttgacaa
     8221 gcttttttaa aatttcttct aattcttcat cagagaaaaa attaatggta atttttcctt
     8281 tattgcctcg ctttttcact tccactttcg tgccaaaaat agcttctaat tttttttcaa
     8341 tttctttttc taaagcatca cccagatcac ttacttgcac tccttgcttt tgttctgaga
     8401 aaacacctaa aggaatttct aaaatgcggc gtgttaaaat ttcggtttct ctcacagtta
     8461 atttccgcga caaaatttca cctaaaagcg ctctttgttt ttcaggatta gtgatggtta
     8521 agagcagtcg cgcgtgcgat tcggtaattt tgccttcttc taaagctcgc tgggcttccg
     8581 acggtaattc taaaagcctt aaagcatttg ccacaaaagc gcggcttttg ccaatccgtt
     8641 ctgcgatttc cctttgagat aatccaaact cctcggctaa ttttgcaaac cctctggctg
     8701 attccaaagg attcagatct gcccgttgaa tattttcaat aatcgccgct tctaattttt
     8761 gtcgctcttc aaacgatggt cgaataattg ccggcactct ttctaatccc aacatttgag
     8821 ccgctcgcaa acgcctttct cctgcaatca attgatactc aactccgcga ccataaggag
     8881 tttctttttc tacgcgcgaa acaatcaatg gttgtaaaat accaaattct ctaatagaat
     8941 cagctaattc ttttagggat tcttcatcaa atcttttacg tggctgataa gggttgggtt
     9001 taattttttc tacttcgatg agatacaccg cttctttaat tgggcctagc tcttctttaa
     9061 ttttctccat ctcctgatct gttattgcat cattgtgcac tgcttgattt aaattatcgt
     9121 caaaattatt tggctctaat tgctgagaat tttcaggttt aagattttga aaaggggctt
     9181 gaagcaacga agaatcttca aataaacatt ctgggtaaat tccctcttta attaaatttt
     9241 ttattccctc agtgacgaca gttttatctt cgggatacgt cacgcctagc cacttggctt
     9301 cagttggcaa aatggtaacc tgcgcttttc cttctcgcat taattgtccc actacctctg
     9361 gaatataaaa ttcatctttt tcggaatttt taatattcat taaaaattta tagaatcgtg
     9421 tttccaattc gtaaaaaatg ctcggcgtaa aaccaaaaaa attcattgaa acagtttcct
     9481 cgcctgtgca attaagccat tgcccgttgt cttcatattt aatgccgtct tcaaatttct
     9541 gaatttttgt tctttcagta atatcgatta agtatccttc gtcagaaatg cggcataggc
     9601 cacgcgaaac agaaccaaaa tcagataaag tattttttaa gcgatagccc accattaaat
     9661 aatggtataa attggaatcg tctgataaat ttttaagagc gtcggccatt ttaatgaatg
     9721 catctcggcc ataaaaatca tcggaattga tcacggcaaa attagataaa acaatatctt
     9781 tacataataa aatagcatga cctgttcccc atggcttttt tctttctggt ggcacgttaa
     9841 accaagtggg taaacctttg tctaattctt gataaatgta agagacatga aaattccttt
     9901 ccgcccgttc tccgattttt tgtttaaaaa cttcctcgat atctttacga atcacaaaaa
     9961 caacatgggt aaagcccgcc tgctttgcat cataaaggga atagtctata ataatttcgc
    10021 cgtttggacc cacaggatca acttgtttta gtccaccata acgacttccc attcctgccg
    10081 ctaaaacaat taaagttaaa tcatcgtttt tcataacttt attctattaa agcaaaaaaa
    10141 taattaattg acaagaaaaa aatttacaag taaaatggaa atagttctta gtaaaaaata
    10201 atttctcaat aattagttcg aaaacttaaa aaatttcgag ccgataaaaa attaagcccg
    10261 ggtggcggaa ctggcatacg cgaaggactt aaaatccttt gtcctttttg gacttgtggg
    10321 ttcgagtccc accccgggca caaacgaata aaatgagttt gggaccggga gcaagccaac
    10381 tgcttggctt gcgtggggac gagaagcgcg cagctatggg cgacgagcga gttggcgagg
    10441 agcccagcga gcggcgggct gcgagcgcga gagcggaagc tcgagcgact tgtagccgag
    10501 tcccaccccg ggcacaaacg aataaaatga gtttgggacc gggagcaagc c
//
LOCUS       DAOSMC010000014         9945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_7726_length_9945_cov_15.433974, whole genome shotgun sequence.
ACCESSION   DAOSMC010000014 DAOSMC010000000
VERSION     DAOSMC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7726_length_9945_cov_15.433974"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..401)
                     /gene="rplL"
                     /locus_tag="PL075_01960"
     CDS             complement(12..401)
                     /gene="rplL"
                     /locus_tag="PL075_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOD96981.1"
                     /translation="MEEIDKKLVNIIEAIEKLNVVELSQLVKALEEKFGVSAAPQIMA
                     VAQGGGGAAAAGAEEKTTVDVILQSAGDAKVQVIKVVKDLTGLGLKEAKDLVDAAPKA
                     IKQGIKPEEAEQIKKTLEEAGATVEIK"
     gene            complement(406..930)
                     /gene="rplJ"
                     /locus_tag="PL075_01965"
     CDS             complement(406..930)
                     /gene="rplJ"
                     /locus_tag="PL075_01965"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOD96982.1"
                     /translation="MLTRAQKEKILNDLVDKINKNPAVLMVDFTGLKVSDLSNLRKEL
                     KNNEMELKVVKKTLVQKMLSHIGINFNIFTFPGSIALIFSPEEGLIASKTVYKFSSEK
                     KEPKLKILGGFINKNFFSVERIIELAKLPSKEVLLSQLVYTLNSLPRTLVGVLNGNIQ
                     KLVIALDAIAKKQS"
     gene            1257..1781
                     /locus_tag="PL075_01970"
     CDS             1257..1781
                     /locus_tag="PL075_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOD96983.1"
                     /translation="MNSAKKQSQKQSEVQKQQARLIHFALKQLGKPYKYAAKKHEIGR
                     VFDCSSFTQYIYRRIGIELPRSTILQAECGRTIPARKKSPYFKKNDLQIGDLLFFKGT
                     KGHFNKKFPNGIGHVMMYLGNGVFIHASGSGPKKNQKIRLETFNNVIHRKDFQIAKRI
                     IKTQTRAHSKPKVK"
     gene            complement(1830..>2006)
                     /locus_tag="PL075_01975"
     CDS             complement(1830..>2006)
                     /locus_tag="PL075_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOD96984.1"
                     /translation="ERKILSMRFGLDDGVIHTLEEVGKEFNVTRERIRQIEQKALEKL
                     KNNPEIQKLLKEEF"
     assembly_gap    2007..2106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2273..4015)
                     /gene="dnaG"
                     /locus_tag="PL075_01980"
     CDS             complement(2273..4015)
                     /gene="dnaG"
                     /locus_tag="PL075_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOD96985.1"
                     /translation="MGIVDEIKEKLDIVEVISEYVKLTKAGANFRALCPFHKEKTPSF
                     MVSPERQIWHCFGCNRGGDIFRFIMEYENVDFGEALRILAKKAGIELKKEDPSFQNQL
                     NKLYEIHEKAAEFFEAQLQNNAAAHSYLKKRGLEEETIKEWQLGFAPNQRDALFRFLL
                     KSGFKTDDIFASGLIIKSEQQSNSYFDRFRNRIIFPIFNHQDRIVAFTGRIFGEEKKE
                     PKYLNSPDSPIFSKGHILYGFSKTKKNIREAERALLVEGQMDFLMSWQSGIKYTVATS
                     GTALTEDQLRILGRMTKNLIIGYDMDEAGRLAAERAIDLARSLDFRVSIISLPEGKDI
                     ADFALLHKEQLPEIIEKAEEAGEYYYRKALTQFNLKDVSGKRKAIEFLLSKIAWIENP
                     VETGEWLKRIAEDFQVREDFLKEDLEKIKKTLDNKGIQNVSATDEVVNKLINGVKTRR
                     ELLSERALALIIKTQVQKDQLVAIVDFLAEEYQKLVREILSDKEKRGGGLNDGGWESI
                     SYLHLLADYEFTDENIDWDLEFQKITLELKKEFFRQTISEKTMLLKKAEEENNIDAIN
                     QYLEELQNLSKEIR"
     gene            complement(4121..>5779)
                     /locus_tag="PL075_01985"
     CDS             complement(4121..>5779)
                     /locus_tag="PL075_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96986.1"
                     /translation="PTPTPTPSPTKTPTPTPTPSPTKTPTPTPTPTKTPTPTPTPTLT
                     PTPTCPSVPHQTKINFSANPALKKTDVVISTDNIPECNSSSASCEWRWSGTVNAINNK
                     KLNGAWTTQFSDTGTKEIKVEVYVKDETICGDSWIKVAYASANLEIVSGGVTPTPTPT
                     PTDTPTPTPTITPTPTPTPTPYHGGGGGGYWLGGTTPTPTVLGTSTCSFVHFESALAE
                     TTCWINGRIGGSCSLNSSDGFNVRLAILANSYPEDIRINISAHQLNNLIDPNVQVGNY
                     KFVGSKIIHIAAEDENCHAIDLENLAVLTINYSDGDIKGFNENSLSISYAPFAPVNWN
                     LLTTYRNISLNSVSAYTAQFGYFGVMGLVEKKVLGVSTVAKSTPSPNFIAWFDPVCIT
                     NEPQNLNNFYTLAALIDRIRNIRIPWYWISLLFNILIILWLLWLYAGKKLLAKYHTAA
                     IAEKGTADSDLAVIFPSADNNSDKQQNYTSISSNKDSHPINSPVINLPGTQSSEIQPI
                     VYLTKENSPSNNPDSQQENQQQKANVLDDEEILKRLKTKLNPLL"
     assembly_gap    5780..5879
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5880..6412)
                     /locus_tag="PL075_01990"
     CDS             complement(<5880..6412)
                     /locus_tag="PL075_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96987.1"
                     /translation="MKKALYPTIIIIGLLIVFVPSLERGENLNISRNNILAMVSQSSC
                     SACSFNTHVVTIAVSPNPVLVNQTVSIKTSIIPECNSPSAYCEWRWSGAVTRVTTNKP
                     TGEWTTSFSTIGTKEIKVEVYVEDATVCSGSWIKVASGSSSFNVYASTPTPTPTPSPT
                     KTPTPTPTPSPTKTPTPT"
     gene            complement(6508..7551)
                     /locus_tag="PL075_01995"
     CDS             complement(6508..7551)
                     /locus_tag="PL075_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOD96988.1"
                     /translation="MKIIIVARNLKGFKKINNTNEDPIIPESSTTQTQVDTYGRVNLD
                     DALAIKKILDKFGHEVKILQIDIDLFEQLKIEKPDLIFNLADDGFYDDPRLEPHVAAL
                     FDILKIPYTGNNYFTLALCQNKARAKEILTYNGILTPKWQVFNNCNRKLNPTLRFPLI
                     VKPIREDGSVGIKERSVVKNEEELKEQISHILDVYQQEALVEEFIDGREFNIGIIGNK
                     KLTVLPIAEIDFSELPPNIPRVVSYRAKWVKQSIVFKKTPVICPANISDKLQKDLEEI
                     SKKCYKIFGCQGYARLDVRYDEKEDKIYVLELNPNPDISEDGGLAREAKTGGMSYENL
                     ILKILDLALKKEF"
     gene            complement(7548..8198)
                     /locus_tag="PL075_02000"
     CDS             complement(7548..8198)
                     /locus_tag="PL075_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOD96989.1"
                     /translation="MFDSYNLLNKILSFYHQAQKQIKIEDSQWQLWCKPRKTLKEKEW
                     VIIESILTQQTNWNNVEQAMANLKNAQKDSLLAIYQTPLNKLKILIRPAGFYQIKAKY
                     LKNVAKFFIQKGGIKNIEKEPAYILRNQLLQIKGVGFETADSILLYAFNRLVFVVDAY
                     TKRWIKEFKISIPSGKRNYSELQQYFVKKIPKNVFLYQKIHAIIVLYYKNKLKISL"
     gene            complement(8174..8554)
                     /locus_tag="PL075_02005"
     CDS             complement(8174..8554)
                     /locus_tag="PL075_02005"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HOD96990.1"
                     /translation="MLKNQNKIKIDHCEFGEISVGGKKFHQILISNNQVFERDEEKLK
                     EMFNSSHFIGDWEKEMLFAANPEVVIIGNGFNGVLRVDDKLDQEAKERGIQLIIGYTP
                     QSVALYNQLVDEGKRVNCLIHTTC"
     gene            complement(8535..8663)
                     /locus_tag="PL075_02010"
     CDS             complement(8535..8663)
                     /locus_tag="PL075_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96991.1"
                     /translation="MSKKIKKNLKVIQTERLKKSLAVVRPARLKSVVHKQHVKKSK"
     gene            complement(8667..9497)
                     /locus_tag="PL075_02015"
     CDS             complement(8667..9497)
                     /locus_tag="PL075_02015"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOD96992.1"
                     /translation="MKNSKLLILIAIIILVIIGVLFLYQQNKIQKTEMSQFQNEEDII
                     HLFSPQPNELITGRFTIKGEARGSWFFEGTAPYYILSSDLATVTSGVITAQGDWMTNE
                     FVPFSGEVDFIPSIESGYLVLKNDNPSGLAEKNLYYMVPLKFQQPATMTVKVFFNNSK
                     LDQEISCNKVFPVEREIVKTSAVARAALEELLKGVSEAEKDQGYFTSINPNVKINSID
                     IKDGIAYVDFDEQLEFQVGGSCRVSAIRAQITETLKQFPSINDVVISINSQAEDILQP
                     "
     gene            complement(9517..>9945)
                     /locus_tag="PL075_02020"
     CDS             complement(9517..>9945)
                     /locus_tag="PL075_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HOD96993.1"
                     /translation="FDASAEKNLNIKPVLSLWAKTVNIKTVPQKEGVGYGFTWIADRD
                     TKIAVVPCGYYEGVPRYLSNIGYFYYHDQPLKIIGRVSMNLTVVDISSIDEIKLEEEV
                     EVFSDDLNKMNSFNYVAKTGNTIPYEILVHLSPQIKRVIK"
BASE COUNT         3052 a         1760 c         1474 g         3459 t
ORIGIN      
        1 ttttttctta tttatttaat ttccactgta gcgcctgctt cttccaatgt ctttttaatt
       61 tgctcggctt cctccggctt aattccttgt ttgatggctt ttggtgctgc atcaactaaa
      121 tcctttgctt ctttcaatcc caaaccagta agatccttta ctactttaat aacttgtact
      181 tttgcatcac cagcgctttg caaaataaca tctactgttg tcttttcttc cgcgcccgca
      241 gcagctgctc cgccaccacc ttgtgcaact gccattatct gaggggcagc tgaaacgcca
      301 aatttttctt ccaatgcctt aaccaattgc gacaattcaa caacatttaa tttttctatc
      361 gcctcaataa tattaactaa ttttttatca atttcttcca taatattacg attgtttttt
      421 agcaattgca tctaaagcaa ttactaattt ttgaatatta ccattgagaa ctccgaccaa
      481 agttcgtggt aaagaattta aagtataaac aagttgagaa agcaaaactt ccttagacgg
      541 taattttgct aattcaataa ttctttcgac tgaaaagaaa tttttattaa tgaacccgcc
      601 cagaattttt aatttcggtt cttttttttc gctactaaat ttataaaccg tttttgaagc
      661 tattaatccc tcttcgggac taaaaattaa agcaatagat ccaggaaaag taaaaatgtt
      721 aaaattaata ccaatatgtg aaagcatttt ttgaactaag gtttttttaa ctacttttaa
      781 ttccatttca ttatttttta actcttttct caaattgctt aaatcagaaa cttttagacc
      841 agtaaaatcc accattaata ctgctggatt tttattaatt ttatccacta aatcattaag
      901 aattttttct ttttgagctc gcgtaagcat aatttaaaat aaaaaacgac tgacaacagc
      961 cgcagactaa aatataattt tgcattaaac tccacacttt tagcctctac aggtcttatg
     1021 tttttagact gcctgttttc tgcggcttta atcaagtata ataaaattaa aaatactgtc
     1081 aagattaatg atatggcact aataaaattt ccggttggaa ttaaaattcc taatttttcc
     1141 ttcttaaatt ttaaattact tttcatctat ttccaaaatt ttaaataaat ccttcattaa
     1201 aaagatacat atattgagtt ttcaatcaat tagattataa tttattatat ttcaatatga
     1261 actcggctaa aaaacaatct cagaaacaat ctgaagtcca aaaacaacag gcaagattaa
     1321 tacattttgc cctcaaacaa ttgggcaaac cctacaaata tgctgccaag aagcatgaaa
     1381 ttggaagggt gtttgactgc tccagtttta ctcaatatat ctatcgacgt attggaatcg
     1441 aattgcctcg atccacaatt cttcaagctg aatgtggccg aactattccg gcgcgaaaaa
     1501 aatctcctta tttcaaaaaa aatgacttac agataggcga tcttttattt ttcaaaggaa
     1561 caaagggcca ttttaataaa aaatttccca acggcattgg tcatgtaatg atgtatttgg
     1621 ggaacggcgt atttatccat gcttctggat ccggaccaaa gaaaaaccaa aaaattagat
     1681 tggaaacatt taataatgta atccatcgca aagatttcca aattgccaaa agaatcatta
     1741 aaacccaaac aagggcacac tccaaaccaa aagtgaaata ataaaaatga ctatgagaat
     1801 gatcgttaat gtttttttct tgcgcatagt taaaattctt cttttaaaag tttttggatt
     1861 tctggattat tttttaattt ttctaaggcc ttttgttcta tttggcggat acgctcgcgt
     1921 gtaacattaa actccttacc cacttcctcc aatgtatgaa taacaccatc gtctaggcca
     1981 aaacgcattg ataaaatttt tcgttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnntttc ttttttctgt cgtttttttt aatattttta gtttttttaa caaaatgtct
     2161 tttttttaat tttttactct tctttaaaat tttactcgtc tttttctttt ttaaagattg
     2221 ttttttcttt tttatatttt tctttttcat atattttaaa atttataaaa gattagcgaa
     2281 tttctttaga taaattctgc agttcttcaa gatattgatt aatagcgtca atattatttt
     2341 cttcttctgc cttttttaaa agcatcgttt tttcagaaat agtttggcga aaaaactctt
     2401 tttttaattc taatgtaatt ttttgaaatt ccaagtccca atcaatattt tcatcggtaa
     2461 attcataatc agccaataaa tgaagataag aaatactttc ccacccacca tcgtttaaac
     2521 ctcctcctct tttttcttta tcgcttaaaa tttctcggac taatttttga tattcttcag
     2581 caagaaaatc aacaatagca actaattgat ctttttgaac ttgcgtcttt ataatcaacg
     2641 caagcgctcg ttcgcttaat aactctcttc ttgttttaac accattaatt aatttattaa
     2701 ctacttcatc tgttgctgaa acgttttgaa ttcccttatt atctaaggtt tttttaattt
     2761 tttctaaatc ttctttcaga aaatcttctc ttacttgaaa atcttctgct attcttttta
     2821 accattcgcc cgtttctacc ggattttcaa tccaggcaat ttttgaaagt aaaaattcta
     2881 tcgcctttct tttgccacta acgtctttta aattaaattg ggttaaagct tttcgatagt
     2941 aatattcgcc cgcttcctcg gctttttcta taatttctgg caattgctct ttatgaagaa
     3001 gtgcaaaatc ggcaatatct ttgccctccg gcaaactaat tatagaaact cgaaaatcaa
     3061 gtgaacgcgc taaatcaatc gctctttctg cagctaatcg cccggcttcg tccatatcat
     3121 aaccaataat caagtttttg gtcatccttc ctaaaatacg gagttgatcc tctgttaaag
     3181 ctgttcctga tgttgctact gtatatttaa ttccactctg ccaactcatt aaaaaatcca
     3241 tctgcccttc aaccaaaaga gcccgttctg cttctctgat attttttttt gttttagaaa
     3301 aaccatataa aatatggcct ttgctaaaaa taggactatc gggagaattt aaatattttg
     3361 gttctttttt ttcttcacca aaaatcctac cggtaaaagc aactatgcga tcttgatgat
     3421 tgaaaatcgg aaaaatgatg cgattacgaa aacgatcaaa ataagaattg ctctgctgtt
     3481 cggatttaat aattaaacct gaagcaaaaa tatcatctgt tttaaaaccg ctctttaaaa
     3541 gaaaacgaaa aagggcgtct cgttgattag gtgcaaatcc taattgccat tcttttattg
     3601 tttcttcttc cagccctctc ttttttaaat aactatgcgc tgccgcattg ttttgtaatt
     3661 gcgcttcgaa aaactccgct gctttttcgt gaatttcata gagtttattc aattgatttt
     3721 gaaaagatgg atcttctttt tttaattcaa tacctgcctt ctttgctaaa attcttagcg
     3781 cttctccaaa atcaacattt tcatattcca taataaatct aaaaatgtca ccacctcgat
     3841 tacaaccaaa acaatgccaa atttgccgtt caggcgaaac cataaaagaa ggagtttttt
     3901 ctttatgaaa agggcaaaga gcacgaaaat tagctccagc ctttgtaagt tttacatact
     3961 cactaattac ctcaacgata tctaattttt ctttaatttc atcaactatg cccataattc
     4021 aatatcctat tataactctt ttgaaaggtt tttcaaaata aacccttaaa acaaaagggc
     4081 ttaataatgt ttgaaattat tcctcgctta tcatttatag ttataaaaga ggatttaatt
     4141 ttgtctttaa gcgctttaaa atttcttcat catctaaaac attcgctttt tgctgctgat
     4201 tttcctgctg agaatcggga ttgttagatg gagagttttc cttggtcaaa taaacaatag
     4261 gttgaatttc tgaagattga gttcctggta aattaatcac cggtgaatta atgggatggc
     4321 tgtctttatt agatgaaatg gaagtatagt tttgttgttt atcagaatta ttatctgcac
     4381 taggaaaaat aactgccaga tcagaatcag ctgttccttt ttctgctatt gctgctgtat
     4441 gatatttggc taataatttc tttcctgcat acaaccataa taaccaaaga attattaaga
     4501 tattaaacag gagagaaatc caataccagg gaatacgaat atttctaatt ctatcaatta
     4561 aagcggctag agtatagaaa ttattcaaat tttgaggttc attcgtaata caaacgggat
     4621 caaaccaagc aataaaatta ggcgaaggag ttgatttagc aacggtggaa actcctaaaa
     4681 cttttttctc aactaagccc ataacgccaa aataaccaaa ttgagctgta taagccgata
     4741 cagaattaag agagatgtta cgataagtag tgagcaaatt ccaattcact ggcgcaaatg
     4801 gcgcgtaaga aatgctcaaa gaattttcat taaatccttt aatgtcgcca tctgaataat
     4861 taatcgttaa tacggctaga ttttctaaat caatagcatg acaattttca tcttcagcgg
     4921 cgatgtgaat aattttagag ccaacaaatt tataatttcc tacttgaaca ttgggatcaa
     4981 ttaaattatt taattgatgg gctgaaatat ttattcgaat atcttccgga taagaattag
     5041 ctaaaatagc cagtcgaaca ttaaagccat cactggaatt tagagaacaa cttccgccaa
     5101 ttcgtccatt aatccaacat gttgtttcag ctaaagctga ttcaaaatga acaaaagaac
     5161 aagtggaagt ccccaaaaca gttggagttg gtgtagtccc gcctagccaa tatcccccac
     5221 ctccgccacc atgataagga gtaggagttg gcgtgggagt tggtgtaatt gtaggcgtag
     5281 gagttggagt atctgtcgga gtgggtgtag gagttggtgt aacgcctcca gaaacaattt
     5341 ctaaattagc tgaagcataa gcaaccttaa tccaactatc accacaaata gtttcatctt
     5401 taacataaac ttctacttta atttctttag taccagtatc gctaaattga gttgtccacg
     5461 cgccatttaa ttttttatta ttaatggcat taaccgtccc tgaccatctc cattcacaag
     5521 aagcgctaga agaattacat tcgggaatat tatcagttga aataacaaca tctgttttct
     5581 ttaaagccgg attagcagag aaatttattt tagtttgatg aggaaccgag ggacaagtgg
     5641 gggtgggtgt aagtgtagga gttggggttg gagttggagt tttggtcggt gttggcgtag
     5701 gagttggggt tttggttggt gatggggttg gagttggagt tggagttttg gtcggtgatg
     5761 gagtcggggt tggagttggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     5881 ttggagttgg agttttggtc ggtgatggag tcggggttgg agttggggtt ttagtcggtg
     5941 atggagttgg ggttggagtt ggagtggaag cataaacgtt aaatgagctg gaaccagaag
     6001 caaccttaat ccaactacca ctgcacacag ttgcatcttc aacataaact tctactttaa
     6061 tttctttagt acctatagta gaaaaacttg tagtccattc accggtgggt ttattggtag
     6121 taactcgtgt taccgcaccc gaccatctcc attcacaata agcactagga gaattacatt
     6181 cgggaataat gctggtttta atgctcactg tttgattaac caagaccgga tttggagaaa
     6241 cagcaatggt aacaacatga gtattaaatg aacaggccga acaagaagat tggctcacca
     6301 tagccaaaat attatttctt gaaatattca aattttcccc tctttccagc gagggaacaa
     6361 aaacaatcag caaaccaatt attataatag taggatataa agcttttttc ataaattaga
     6421 agctaaaaaa atatcatcag aatttttctc tgatgattat attatattta tttttaaaaa
     6481 aatgtcaaca cccccccttg ctgacagcta aaattctttc tttaaagcta aatctaggat
     6541 ttttaaaatt aaattttcgt aagacattcc tcctgttttt gcttcgcgcg ctaatccacc
     6601 gtcttcggaa atatcaggat taggatttaa ttccaataca taaattttat cttccttttc
     6661 gtcataacgt acatctaatc gggcataccc ctgacaacca aaaattttat agcatttctt
     6721 cgaaatttct tctaggtcct tctgcagctt atcgctgatg ttagctggac aaatcactgg
     6781 cgttttttta aaaacaatac tctgtttcac ccattttgct cgataactaa ccactctagg
     6841 gatattagga ggtaattcac taaaatcaat ttcggcaata ggtagaacag ttaatttttt
     6901 attgcctata ataccgatgt taaattcgcg gccatcaata aattcttcaa ccagcgcctc
     6961 ttgttgatag acgtcaagaa tgtgacttat ttgctctttt aattcttctt cattttttac
     7021 cactgagcgc tcttttattc ctacactgcc atcttctcga atgggcttca cgattaatgg
     7081 aaatcttaag gtaggattga gttttctatt acaattatta aaaacttgcc actttggtgt
     7141 caaaatgccg ttataagtta aaatttcttt ggctcgagct ttattttgac aaagcgctaa
     7201 cgtaaaataa ttatttccgg tataaggaat ctttaaaata tcaaacagcg ctgctacatg
     7261 aggttctaag cgcggatcat cataaaaacc atcatcagct aaattaaata tgaggtccgg
     7321 tttttctatt tttaattgtt caaataaatc aatatctatc tgcaatattt ttacttcgtg
     7381 gccgaactta tcaagaattt ttttaatagc caaagcatca tcgagattaa cacgcccata
     7441 tgtatcaact tgagtttgag tggtagaact ttcaggaata atagggtctt cgtttgtatt
     7501 attgattttt ttaaaacctt ttaagtttct tgcaacgata ataattttca taaagaaatt
     7561 tttaatttat ttttataata caaaactatt atagcatgaa ttttctgata aagaaaaacg
     7621 ttcttgggaa ttttttttac aaaatattgt tgaagttctg aataatttct tttcccgctc
     7681 ggaatactaa ttttaaattc tttaatccag cgcttagtat aagcatccac aacaaatacc
     7741 aaacgattaa aagcataaag taagattgag tcagcggttt caaatcccac tcctttaatt
     7801 tgaagtagtt ggtttcttaa aatataagca ggctcttttt caatattttt tattccccct
     7861 ttttgaataa aaaatttagc tacatttttt aaatattttg ccttaatttg ataaaatccc
     7921 gctggtcgaa ttagtatttt taatttatta agcggtgttt gatatatggc taacaatgaa
     7981 tctttttggg cattctttaa attagccatg gcttgctcaa cattattcca atttgtttgt
     8041 tgagtcaaaa ttgactcaat aattacccat tctttttcct ttaatgtttt tctcggctta
     8101 caccataatt gccattggga atcttctatt tttatttgtt tttgggcttg atgataaaaa
     8161 gataaaattt tatttagcaa gttgtatgaa tcaaacaatt aactcttttt ccctcgtcga
     8221 ccaattgatt ataaagcgcc acagattgag gcgtataacc aataataagt tgaatccctc
     8281 tttctttggc ttcttgatct agtttatcat caacacgcaa aacgccatta aaaccattgc
     8341 caataatgac tacttctgga ttagcggcaa atagcatctc tttttcccaa tcaccaataa
     8401 agtgtgacga attaaacatc tccttcaatt tttcttcatc gcgttcaaaa acttgattat
     8461 tagaaatcaa tatctgatga aattttttcc cacccacact aatttctcca aattcacaat
     8521 gatcaatttt aattttattt tgatttttta acatgttgtt tgtgtacgac gctttttaat
     8581 cttgctggac gtacaacagc aagacttttt ttaagccgtt cggtttggat aacttttaaa
     8641 ttttttttaa tttttttact cataatttat ggttgtaaaa tatcttccgc ttgactgttg
     8701 atagaaatga caacgtcgtt aatggatgga aattgtttta atgtttctgt aatctgagct
     8761 cgaatggcag aaactcggca cgaacctcca acttgaaatt ctaattgctc atcaaaatca
     8821 acataagcta tgccatcttt tatatcaata gagttgattt taacattagg gttaatgctt
     8881 gtaaagtatc cctgatcttt ttctgcttcg cttactcctt ttaataattc ttctaacgca
     8941 gctctggcaa cagccgaagt tttaactatt tctcgttcca ccggaaaaac tttattacaa
     9001 gaaatttctt gatctaattt gctattatta aagaacacct taactgtcat agtggccggt
     9061 tgttgaaatt ttagaggcac catataatat aagttctttt ctgctaaacc tgacggatta
     9121 tcatttttta aaacaagata accgctttca atagatggaa taaaatccac ttctccagaa
     9181 aaagggacaa attcattagt catccaatct ccttgagcag tgataacccc cgatgtcact
     9241 gtagccaaat ctgacgataa aatataatat ggagcggtac cttcaaaaaa ccaactgccc
     9301 cgcgcttcgc ctttaatggt gaatcgtcct gtaatcaatt cgttaggttg aggactaaat
     9361 aaatgaatta tgtcttcttc gttttgaaat tgcgacattt ctgttttttg gattttgttt
     9421 tgttgatata aaaacaaaac tccgataata acaagaataa taatagcgat taaaattaaa
     9481 agttttgaat ttttcatatg atataaataa tcgactttat ttaataacac gtttaatctg
     9541 cggtgataaa tgcactagaa tctcataggg aatcgtattg cctgtttttg caacataatt
     9601 aaatgaattc attttattaa gatcatcaga aaacacctct acctcttctt ctaattttat
     9661 ttcatcaatc gatgaaatat ctacaaccgt taaattcata ctcactcgac caataatttt
     9721 taaaggttgg tcgtgataat aaaaatagcc gatattgctg agatagcgag gaactccttc
     9781 ataatatcca caagggacaa cagctatttt tgtgtctcga tcggcaatcc atgtaaaacc
     9841 atagccgact ccttcttttt gaggaacggt tttaatattt acggtcttgg cccaaagcga
     9901 taaaacaggt ttaatattta gatttttttc cgccgaagca tcaaa
//
LOCUS       DAOSMC010000015         9690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_7954_length_9690_cov_21.906072, whole genome shotgun sequence.
ACCESSION   DAOSMC010000015 DAOSMC010000000
VERSION     DAOSMC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7954_length_9690_cov_21.906072"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1314
                     /locus_tag="PL075_02025"
     CDS             283..1314
                     /locus_tag="PL075_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96994.1"
                     /translation="MRKGLNIRAIGGASEKAKKEIKHDIERALFEHFDSLSQKEQEMF
                     IQFERAKSEKELALIQLADRETSRLMQEAGVEPYSVPFENFHIVSPDIYKKLFDADTV
                     AASFCVKQGIIFNDERFRNNPVYLGISVFHELLHLKGHLSIEVEEEESASKIHGSLYR
                     HGVTAYSSQQTINKNQFHFHFSGLHEAIVSEAEKQFLPKLLDLPELALEKRWLNSEKA
                     KNLKKKIAIEKGIPEEDIIWVNEDGSDYEDFSYRAQRKVLKYVCEEIKKQYPEQFKDS
                     SEVYKLFLKAHFTGQLLPIARLMEKTFGEGSFRLLGNMETSNESGILHLESLQKARAR
                     YLRESLKEK"
     gene            complement(1384..2799)
                     /locus_tag="PL075_02030"
     CDS             complement(1384..2799)
                     /locus_tag="PL075_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOD96995.1"
                     /translation="MPKKSSTLQKKALKLILDNVANLSDENLIQIIEIGEKYLIENPT
                     LKQQAEDIKKLFEQKHPALDLVRRTLNSISKECRDKLIENLIINAMVIENQRRQKLAK
                     ELGFGLPWFFVMSPTAQCNLNCRGCYAGRYIKDEGLPFELVDRILNEAKDLGIYFVVV
                     SGGEPYFWPHILKMFEKHNDMYFMTYTNGTLLTKEMAKKLAKLGNVAPAISIEGFEKE
                     TDDRRGPGTFKKIMEAMDNLKEAGVPFGFSVTPTKLNIEALMSDEFVDLMIKKGCSFG
                     WYFQYVPVGRKPDVNLMVAPEDRNRLRMRTNEIRNTKPLFVGDFWNDGPYAGGCIAGA
                     RPGGYFHINCNGGIEPCAFLQFSTDNIKDKKLLDVIRSPFFQAFQEAQPYCENGNLLT
                     PCALIDNPEILRKLVREYNAKPSYEGADDAICDPTICHFLDEYSKAYKKIADPIWENG
                     MNKKHKHWKEKAAESAKKTNK"
     gene            2938..3438
                     /locus_tag="PL075_02035"
     CDS             2938..3438
                     /locus_tag="PL075_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD96996.1"
                     /translation="MTQQDQIPIDRQQEQKEKIPLDDSEFWVIDDSTEIIRSLMGMWQ
                     KITDQYHLKGKIFSTAAEALAEINRRKEEKKSLPKVIFMDAWLDGDTLEELQYGGNII
                     KKIREMEDVLQPLIIAHSSEDKYNEALKTAGADLIIKKGDYKSSKMFLENLGQSKQNK
                     NEKEKD"
     gene            3469..4098
                     /gene="purN"
                     /locus_tag="PL075_02040"
     CDS             3469..4098
                     /gene="purN"
                     /locus_tag="PL075_02040"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOD96997.1"
                     /translation="MSQETKKKIKICALIGRGGRLPAIYNCVKDYPLAELALVVSHKK
                     ESPGIEWAKKQGIEAFYFRLSDWKAQGKDRLAFDDELGKMLKERGIDLVVMAGWDLVV
                     SKEFLKYFPNAVINIHPALSPAFPGLDAPKQALEYGVKITGCTLHFVDEGVDTGPIIF
                     QRAVKIEDNDTPESLEEKIHQQEEEILCEGMRAFAEGRIKINGRKVTIL"
     gene            4095..5462
                     /locus_tag="PL075_02045"
     CDS             4095..5462
                     /locus_tag="PL075_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOD96998.1"
                     /translation="MSDFSKKVSLPWLILILVTAVALAALIYYGPKKEVKNYTIIING
                     SSDNNIEFQYGVWPQLGDVDFFNNVLEKFIEQKMDFIMADLDQMKLKVFKQGEIIKEV
                     PIGAKGREGSWWETPVGLYKIEAKHKKAYSNFAGVYMPYSLVFEGNFLIHGWPYYPSG
                     KPVSSTYSGGCIRLFDEDAKEIYNLAEVGMPVLVFKKAFEVENSTYQYKIPELSAQAY
                     LAADLKNNFIFLEKSRNQILPIASITKLITSLVVLDYTNLEHYARVPQEAIIFTSIPR
                     LKAGQKIPIYELLALLLAESSNEAGNTLAAFLGSEKTINLMNNQAKSIGMTNSHFVDP
                     NGVSLENVSTPLDLYQLAKYLYFNRSFILKMTKGIFDNDYYGQPSYNLKNLNLFADDP
                     SFVGGKMGLTSGSGETMLAIFELSIGNSQKRPVVFVVLNSKDVYTDIVKMVEFVKNHY
                     QFENE"
     gene            5455..5973
                     /locus_tag="PL075_02050"
     CDS             5455..5973
                     /locus_tag="PL075_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOD96999.1"
                     /translation="MSNWDYLIFQKINSAAGSMRWCDQVMVFLAEYLMYILLVAGLIY
                     LFFAYQKQLKALIVAAAAIILSRGFITEIIRFFYYRPRPFLTHPVNQLLTHDISGSFP
                     SGHMTFIFPLIALVYLKNKKLGVVFMVLGVLMGLARIYCGVHYPSDIFAGIIIGVFCG
                     WFVEKIATKNLK"
     gene            complement(6110..6541)
                     /locus_tag="PL075_02055"
     CDS             complement(6110..6541)
                     /locus_tag="PL075_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD97000.1"
                     /translation="MAFKLFKSKGEDVEKDIEIKNTPSSNEEEDVLTGEGQLMVDVYQ
                     TPTEIVIKSAVAGVKPEDIDISIVNDMITIKGKRELDEEVRANDYFYQECYWGSFSRS
                     VILPVEIDGDRVSASLRNGILTIRLPKLDKNRVKKVKIKAE"
     gene            complement(6531..7316)
                     /locus_tag="PL075_02060"
     CDS             complement(6531..7316)
                     /locus_tag="PL075_02060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOD97001.1"
                     /translation="MISGIFYLFFLLALSITPSLIWLNYYLKKDFKPEPRRWLSIAFA
                     AGVLITIIIGIAESALFGEWTELPNNQNLINIVFFLFLPFLEETGKFIATFFSSFKNP
                     HFRDETTDPIIYAVVTGLGFAAAENLKVCFSILQENMVLVNWQNLASFSLSAPIINTL
                     IITMFIRLLSAVFLHASSCSIFGYFWAIGRILKKKKYAIIGLLVFGIIMASLLHSCYN
                     YLIMKTVENLYFAFGVAALLIISNYVMSRAFKQVESYQKTYGV"
     gene            complement(7313..9514)
                     /locus_tag="PL075_02065"
     CDS             complement(7313..9514)
                     /locus_tag="PL075_02065"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOD97002.1"
                     /translation="MPELEKTYQPNKVEEKIYKMWESSGYFNPDNLPTSNKKTFSIIM
                     PPPNANGSLHLGHALTMTIEDIMTRYQRLRGLKTLWLPGADHAGFETQVVFEKELEKR
                     GTSRFEILKEKDGRKKLYQMIWDFTQNNKLQMEKEVKKLGASCDWSREKFTLDPSIVK
                     IVYQVFERMYNDGLIYRDYRIVNWCPKHQTALSDLEINWEERNDKLYYVKYFLVDDPQ
                     QFITVATTRPETIPGDFAIAVHPQNKQYKKFIGKLVTNPLIKNRGKYGDQIKVISDDL
                     VEKEFGTGALKITPAHDAVDFEIGKKHQLPLDYQIIGWGGKMNELSGELKELPILAAR
                     QKSVEILEKAGALEKIEDYKHQVSICYKCKTTIEPLPRAQWFVKMTEPPKNGKKSLRD
                     LAVEAVKSKKIKFITKRFEKIFYHWMQNIRDWNISRQIVWGIPIPIWYCTKCNKEVVY
                     IEEGEPKKCPHCGNVTFKKEEDVFDTWFSSGQWPYATLGYPNNEDFKKFYPTSVMETG
                     WDILFFWVARMIIFGIYTTGQPPFKYVYLHGLIRDKDRQKMSKSKNNVIDPLGMIDLY
                     GCDALRMSLIFGNAPGNDPIVSEDKTRAFRNFANKIWNASRFVLMNLDDFDGSIQPKL
                     SKEDKKILKDFEKFTADVTREMDKFNFHIAAEKIYHYFWHVFADKIIEHEKPRLQTEN
                     RLQAQYLLITLLKNLLILLHPFMPFITEEIYQMLPNKDRELLMIEDWPYKI"
BASE COUNT         3102 a         1634 c         1781 g         3173 t
ORIGIN      
        1 ctcgcgagcg gcggcctgcg agaatttgcc gtcaggcaaa ttacttgtag gcgaatcctc
       61 ccgcctccgc ttttataaaa tattcagagc aagccaactg cttggcttgc gtggggatga
      121 gaagcgcgca gcgttaggcg agttttccaa ccgaaagttg gaaaggcgag cctcgcgagc
      181 ggcggcctgc gagaatttgc cgtcaggcaa attacttgta ggcgaaaaaa acaggggagc
      241 tggcactagc atttttgtat aaaatatgtt ataataaaga atatgagaaa aggactaaat
      301 attagagcaa ttggaggagc gtcggaaaag gcaaaaaaag agattaaaca cgatatcgaa
      361 cgcgctttgt ttgagcattt tgattctttg tctcaaaaag aacaggagat gtttatccaa
      421 tttgagagag ccaaatccga aaaagaattg gcacttattc aattagcaga cagagaaact
      481 tctcggttga tgcaagaagc aggcgttgag ccgtatagtg tgccttttga aaattttcat
      541 attgtctccc ccgatattta taaaaagtta tttgacgctg atactgtagc ggctagtttc
      601 tgcgttaagc aaggaataat ttttaatgat gaacggttta gaaataatcc tgtttatttg
      661 ggcattagcg tttttcacga attacttcat ttaaaaggac atttatcaat agaggttgaa
      721 gaagaagaaa gtgcgtctaa aattcatggt tcgctttatc gccatggcgt gacggcttat
      781 tcttctcagc aaactattaa taagaatcaa tttcattttc atttttctgg tcttcacgag
      841 gccattgttt ctgaagcgga aaaacaattt ttacccaaat tgcttgattt gccagaattg
      901 gctttagaaa aaagatggtt aaattctgaa aaagctaaaa atttaaagaa aaaaattgcc
      961 attgagaaag gcatccccga agaagatatt atttgggtca atgaagacgg tagtgactat
     1021 gaggattttt cttaccgagc ccagcggaaa gttttaaaat atgtttgtga ggaaatcaaa
     1081 aaacaatatc cagaacaatt taaagattcc agtgaagttt ataagctttt tctcaaagct
     1141 cattttactg gtcaactttt gcctattgct cgattaatgg aaaagacttt cggggaaggc
     1201 agttttcggt tattgggcaa tatggagact tctaatgaaa gtggcatcct ccatttagaa
     1261 tctttacaaa aagcgcgggc gcgttatttg cgtgaatctt taaaagaaaa ataattactt
     1321 taattgagat gagaaaaact gcgggcattt gcccgcagtt tttctcatct ctaattcctt
     1381 ttattattta ttagtttttt tcgcactttc cgctgctttt tctttccaat gtttatgttt
     1441 tttattcatg ccgttttccc aaatcggatc agcaattttt ttataagctt tcgaatattc
     1501 atctaagaaa tgacaaatag ttggatcgca aatagcgtca tccgcccctt cataagatgg
     1561 tttagcatta tattctctta caagttttcg taaaatttct ggattgtcaa ttaaagcaca
     1621 aggggtaaga agatttccgt tctcgcaata aggctgagct tcttggaagg cctggaaaaa
     1681 aggagaacga ataacatcca gcagtttttt atctttaatg ttatcagttg agaattgcaa
     1741 gaaagcgcat ggttctattc caccattgca gttaatgtgg aaataaccac caggtcgagc
     1801 gccagcgatg caaccgccag cataaggacc gtcattccaa aaatcgccaa caaaaagagg
     1861 ctttgtattt cgaatttcat tagttctcat tcgcaatcta tttctatctt cgggcgcaac
     1921 cattaaatta acatcaggct tacgaccaac gggtacgtat tgaaaatacc aaccaaaaga
     1981 acagcctttt ttgatcataa gatcaacgaa ttcgtcagac atcagtgctt caatatttaa
     2041 tttcgttggt gtgaccgaaa aaccaaaggg cacacccgct tctttaagat tatccattgc
     2101 ctccataatt tttttaaatg ttcctgggcc acgacgatca tctgtttctt tttcaaatcc
     2161 ttcaatagaa atggcagggg caacattgcc taattttgcc agttttttgg ccatttcttt
     2221 cgttaataaa gtgccattag tgtaggtcat aaaatacata tcgttgtgtt tctcaaacat
     2281 tttgagaata tgtggccaaa agtaaggttc accgcccgaa actactacaa aataaattcc
     2341 caggtcttta gcttcgttaa gaattcgatc cactaattca aaaggtaacc cctcgtcttt
     2401 aatatatctt ccggcatagc agccacggca attaagattg cattgtgctg tagggctcat
     2461 aacaaaaaac caaggcaatc caaaaccgag ttctttggcg agtttttggc gccgttgatt
     2521 ttcaattacc attgcattga tgattagatt ttcaattaat ttatcgcgac attcttttga
     2581 aatactattt aaggttcggc gcaccaagtc taaagctgga tgtttttgtt caaaaagctt
     2641 tttaatatct tcggcttgtt gttttaaagt cggattttca attaaatatt tttctccgat
     2701 ttcaataatt tgaatcaaat tttcatcaga gagattagcg acattatcca agataagctt
     2761 gagagctttc ttttgtaagg ttgaactttt tttaggcata aaaaatgttt ttactcggcc
     2821 attgaaaaat aggagattgt attatacaat actctctttt taagatagcc ctttttttaa
     2881 gaaagtcaat tgtctcaatt atttgaatta ttattgcaaa gatttataat aaaattaatg
     2941 acccaacaag accaaattcc tattgatagg cagcaagaac aaaaagaaaa aattcctttg
     3001 gatgattcgg agttttgggt tattgatgat tcaactgaaa taatacgcag tttaatggga
     3061 atgtggcaaa aaataacaga tcaatatcat ttaaaaggga aaatttttag tacggcggcc
     3121 gaagcattag ctgagattaa tcggagaaaa gaagaaaaga aatccttacc aaaggtaatt
     3181 tttatggatg cttggttaga tggtgatact ttagaagaat tgcagtacgg cgggaatatt
     3241 attaaaaaaa ttagagaaat ggaagatgtt ttgcagccat taattattgc ccatagttct
     3301 gaagacaaat ataacgaagc gttaaaaaca gccggagctg atttaataat taaaaaagga
     3361 gattataaaa gttcaaagat gtttttagaa aatttagggc aatctaagca aaacaaaaac
     3421 gaaaaagaga aagattaatt tgatagatta aaaaactgtt tataattaat gtctcaagaa
     3481 acaaagaaaa aaattaaaat ttgtgcctta attggtcggg gcgggcgatt gccggctatt
     3541 tataattgtg tcaaggatta tcctttggca gaattagcat tggtcgtttc tcataaaaaa
     3601 gaatcacccg gcatagaatg ggcaaaaaaa caaggcatcg aagcctttta ttttcgtctt
     3661 tctgattgga aggctcaagg caaggatcgt ttggcttttg atgatgaatt agggaagatg
     3721 ctgaaagaac ggggaattga tttagtggtg atggctggtt gggacttagt tgtttccaaa
     3781 gaatttctta aatattttcc taatgcggtt attaatattc atccggctct atcaccagct
     3841 tttccaggac tggatgctcc gaagcaagct ttggaatatg gagtcaaaat tacaggttgc
     3901 accttacact ttgttgatga aggtgtagat acgggtccaa ttatttttca acgtgcagtg
     3961 aaaatagaag ataacgacac ccccgaaagt ttagaagaaa aaattcatca gcaagaagaa
     4021 gaaattttat gtgaaggaat gagagctttt gctgaaggtc gtattaaaat taatggccgt
     4081 aaagtaacta ttctatgagc gatttttcta aaaaagtttc tttaccatgg cttattttaa
     4141 ttttagtaac agcggtggcc cttgcggctt taatttatta tggccccaaa aaagaagtta
     4201 aaaattatac cattattatt aatggttcgt ctgataataa tattgaattt caatatggag
     4261 tatggcctca gttaggcgat gtggattttt ttaataatgt tttagaaaaa tttattgaac
     4321 aaaaaatgga ttttataatg gctgatttag atcaaatgaa actcaaggtt tttaaacaag
     4381 gtgaaataat taaagaagtg ccaattggcg caaaaggccg cgaaggttcg tggtgggaaa
     4441 cgccagttgg tttatataaa atcgaagcca aacacaaaaa agcttattct aattttgccg
     4501 gggtttatat gccttatagt ctagtttttg aaggtaattt tcttattcac ggttggcctt
     4561 attatccatc aggcaaaccg gtatcttcta catattccgg cggttgcatc agattatttg
     4621 atgaagatgc taaagaaatt tataatttag ccgaagtggg tatgccggtt ttagttttta
     4681 aaaaagcgtt tgaggtagaa aattctactt atcagtataa aattccagag ttaagtgccc
     4741 aggcttattt agcggctgat ttaaaaaata attttatttt tttagaaaag agtcgcaatc
     4801 agattttgcc tattgcttca attactaaat taatcacttc attagtagtt ttagattata
     4861 ctaatttaga gcattatgca cgcgtgcctc aagaagcaat aatatttact tcaattcctc
     4921 gtttaaaagc aggtcagaag attcccattt atgaattatt ggctttactt ttggcggaat
     4981 cttcaaatga ggcaggaaat actttagcag cgtttttagg atcagaaaaa acaattaatt
     5041 taatgaacaa ccaagctaaa agcattggca tgaccaatag ccattttgtt gaccctaatg
     5101 gcgtaagctt agaaaatgtt tcaacgccgc tcgatttata tcagttagcc aaatatcttt
     5161 atttcaatcg cagttttatt ttgaaaatga ctaagggtat ttttgataac gattattatg
     5221 gtcaaccatc atataatctt aaaaatttaa atctttttgc tgatgatcca agttttgttg
     5281 gaggaaaaat gggattaaca tcaggaagcg gcgaaacgat gttggctatt tttgaattat
     5341 caataggtaa ttctcaaaag cggccagttg tttttgtggt attgaattct aaggatgttt
     5401 acaccgacat tgttaaaatg gtcgaatttg ttaaaaatca ttatcaattt gaaaatgagt
     5461 aattgggatt atttaatttt tcaaaaaatt aatagtgcag ctggatctat gcgctggtgc
     5521 gaccaagtta tggttttttt agcagaatat ttaatgtata ttcttttggt tgccggattg
     5581 atttatcttt tttttgcata ccagaaacaa ttaaaagcgc tgattgtagc agctgcggct
     5641 attattttga gtcgtggttt tattacagaa atcatccgtt ttttttatta tcgcccgcgg
     5701 ccatttttaa ctcatcccgt taatcaattg ttaactcatg atattagcgg ttcttttcct
     5761 tcgggtcata tgacttttat ttttccgctt attgctctgg tttatttaaa aaataaaaaa
     5821 ttaggcgtgg tttttatggt tttgggcgtt ttaatgggat tagcgcgtat ttattgcggc
     5881 gttcattatc cgtccgatat ttttgccggc ataattatag gcgttttttg tggttggttt
     5941 gtagaaaaaa tagccacaaa aaatttgaaa tgatcacaga aaaattccac attttatcaa
     6001 ttcgtttttc ataaaaagaa aatagccgcc ggaaaatttc tgcgaaattt tccggcggct
     6061 atattgtctg ctatcaattt taattttact ttttgtactt ggaaactttc tattccgctt
     6121 taatttttac ttttttcact ctgtttttat ccagtttagg aaggcgaata gttaaaatgc
     6181 catttcttaa actagcgcta acgcgatcac catcaatttc tactggcaaa ataactgaac
     6241 ggctaaatga tccccaatag cactcctgat aaaaataatc attcgctcta acctcctcat
     6301 ctaattcccg cttacccttg atggtaatca tatcgtttac aatggagata tcaatatcct
     6361 cgggttttac tccagccacg gcggatttaa taacaatttc cgtcggcgtc tgataaacat
     6421 caaccattaa ctgaccttcc ccagttaaaa catcctcttc ttcattagac gaaggagtgt
     6481 ttttaatttc aatatctttt tcgacgtctt cgccttttga tttaaataat ttaaacgcca
     6541 tatgtttttt gataagactc tacttgttta aaagctcgac tcattacata attagaaata
     6601 attaataaag ccgcaacacc aaaagcaaag taaagatttt caacagtttt cattataaga
     6661 taattataac aagaatgcaa aagagaagcc attataattc caaaaacaag caatccaatg
     6721 atagcatatt ttttcttttt taaaattcgg ccgattgccc aaaaataacc aaaaatagag
     6781 caagaagaag catggagaaa aacagccgaa agaaggcgaa tgaacatagt aataataagc
     6841 gtattgataa tcggagcaga tagagaaaaa gaagctagat tttgccaatt taccaaaacc
     6901 atattttctt gaaggattga aaaacagact tttaaatttt ctgccgctgc aaatcctaat
     6961 ccagtaacaa cagcataaat aataggatcc gttgtttcat cgcgaaaatg agggttcttg
     7021 aaagagctaa agaatgtggc tatgaacttg ccggtttctt ctaaaaatgg cagaaacaaa
     7081 aagaaaacga tatttattaa attctgatta ttaggcaact cggtccattc tccaaacaag
     7141 gcactttcag caataccaat aataattgta attaaaacgc cagcagcgaa agctatactt
     7201 agccaacgtc gaggttctgg cttaaagtct ttttttagat aataattcaa ccaaattaaa
     7261 ctgggcgtga tggatagagc gagaagaaaa aaaagataaa aaatgccaga aatcatattt
     7321 tgtaaggcca atcctcaatc attaataatt ccctatcttt attaggaagc atttgataaa
     7381 tttcctcagt aataaagggc ataaaaggat gcaggagtat gagtaaattt tttaataggg
     7441 tgatgagaag atattgagct tggagtcggt tttctgtttg gaggcgcggt ttttcgtgct
     7501 caataatttt atctgcaaaa acatgccaga aataatgata aattttttct gcagcaatat
     7561 ggaaattaaa tttatccatt tctcgagtta catcagcagt gaatttttca aaatctttta
     7621 agattttttt atcctcttta gataattttg gttgaatgct tccgtcaaaa tcatcaagat
     7681 tcataaggac aaaacggcta gcattccaaa ttttattagc aaaatttcga aaagcgcgtg
     7741 ttttatcctc agagacaata ggatcgttgc cgggcgcatt gccaaaaatt aaagacattc
     7801 ttaaagcgtc gcacccatac aaatcaatca ttcctaatgg atcgatgaca ttattttttg
     7861 attttgacat tttctggcga tctttatcgc ggattaatcc atgaagatat acatatttaa
     7921 agggcggttg gccagttgta taaattccaa aaataatcat gcgcgccacc caaaagaata
     7981 aaatatccca acctgtttcc ataacgcttg taggatagaa ttttttaaaa tcttcattat
     8041 tgggataacc aagcgtggca taaggccatt gaccagaaga aaaccaggta tcaaaaacat
     8101 cctcctcttt tttaaaggtt acattgccgc aatgggggca tttttttggt tcgccttctt
     8161 caatataaac cacctcttta ttgcattttg tgcaatacca gatcggaatg ggaatgcccc
     8221 aaacaatttg gcgtgaaata ttccagtctc gaatattttg catccagtga taaaaaattt
     8281 tttcaaagcg tttggtgata aatttaattt tttttgattt gacagcttct actgctaaat
     8341 cgcgcaagga ctttttgcca ttttttggcg gctcagtcat tttgacaaac cattgcgctc
     8401 gcggcaaagg ctcaatggtg gttttgcatt tataacatat tgaaacttgg tgtttataat
     8461 cttcaatttt ttccaaagcg ccagcttttt ctaaaatttc tacggatttt tgacgagctg
     8521 ccaaaatagg caactccttt aattctcccg acaattcatt catttttcca ccccaaccaa
     8581 ttatttgata atcgagcggt aattgatgtt ttttgccaat ttcaaaatca actgcatcgt
     8641 gcgctggagt aatttttaaa gcaccggtgc caaattcctt ttcaaccaaa tcatcgctga
     8701 tgaccttgat ctgatcgcca tactttccgc gatttttaat aaggggatta gtgactaatt
     8761 ttccaataaa ttttttgtat tgtttgtttt ggggatgaac agcaatagca aaatcaccgg
     8821 ggatggtttc aggccgagtg gttgcgacgg taataaattg ctgaggatcg tcaaccaaaa
     8881 aatatttcac ataatataat ttatcatttc gttcctccca attgatttct aaatcagaaa
     8941 gcgctgtttg gtgtttggga caccaattga caatgcgata atcacgataa attaatccat
     9001 cattatacat gcgctcaaat acctgataaa caattttaac aatggaaggg tcgagcgtaa
     9061 atttttcccg actccaatca catgaagcgc ctaatttttt cacctctttt tccatttgta
     9121 atttattatt ttgggtaaaa tcccagatca tttgataaag tttttttcgg ccgtcttttt
     9181 ctttcaaaat ttcaaagcgg ctcgttcctc ttttttctaa ctctttttca aaaaccactt
     9241 gcgtttcaaa acccgcatga tcagcccctg gcagccataa tgttttgagt ccgcgtagac
     9301 gctgatagcg agtcataata tcttcaatag tcatggtgag cgcatggcct aagtggagtg
     9361 atccgttagc attaggcggc ggcataataa tggaaaatgt ttttttatta gaagtaggca
     9421 gattatccgg gttaaaataa ccggaactct cccacatttt ataaattttt tcttcaactt
     9481 tattaggctg ataagttttt tctaattcag gcatatgata ttaaatttta atataaaaaa
     9541 ttagaataaa caaggcagcg cttatttaat ttgttatttt gagagaaatt ggaaaataga
     9601 aggcggcgga aaaattcttt tagaattttt ccgccgcccg tttcgtcttc cctccattat
     9661 ttcaaacatt ttatttatgt cattctgaac
//
LOCUS       DAOSMC010000016         8893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_8832_length_8893_cov_18.874859, whole genome shotgun sequence.
ACCESSION   DAOSMC010000016 DAOSMC010000000
VERSION     DAOSMC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8832_length_8893_cov_18.874859"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="PL075_02070"
     CDS             <1..2058
                     /locus_tag="PL075_02070"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOD97003.1"
                     /translation="TGYSIVEAKKTTMNLSADKRVINKVGEPVTLTWAVENSLLSTIG
                     GGGGGRADRMEEAVDEGGCNSVWDIINAECSPLDFNKITPQNNFANSFINLSNNLFAK
                     ITSIFMQPTIAQTTPQPVVFLFVGNAACHPEGDASDCDFAPNSYYPVLTNSGSLTVYP
                     QSDTNFYLIVYGVSGEGPKFFVSEPLEVSIEEDEQEEGRVDLKAKEDSSGHDYTDGPI
                     TISRGADISLKWSGEENLDSCTATASPENSAWQGKVATTGTKTIYGVTNSVIFKLDCT
                     LKNGNSVSDSVTVNVVEPPALGTIEVKARLFNGSNVSDYTGPVKYTITGPQSLTGETP
                     QSFSVSAGTYTLTYVSGGPNDAVCITHPQTKVVTARETTTFYLDFEVQNLCSISVDYQ
                     VPGSTAEWDGKASYVLIGPTILTGNSLPYSFNQIPPGVYYISYISGAPENLIFSGVLE
                     GNGQVCYPKEPLQFTLQFAEPGATPPVPLPSPEIGDNPPSAKIGCAADNSISCTGTID
                     GDANTPTITLYNASTDPDRDIKSCTWIIQKTGGEPISKNTCQPVSIYDDTGSYGVTLT
                     VTDSRGNTDSAQLTATVKNVTELIAKFVWDPQIPTVGKEVKFYDRSLTPSGTSLKTWL
                     WSFEDGSPATSTSPNPEVIFGATGPKAVTLTVTNNVNNKAVITQIIDVRNIAPQWKEV
                     IPK"
     gene            2077..3330
                     /locus_tag="PL075_02075"
     CDS             2077..3330
                     /locus_tag="PL075_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97004.1"
                     /translation="MQIAAQERIAKILRCPSQTIFELEKKMEAISGKKGVFEALVQEN
                     SEIIADRLKNLAVKNQTAEEISHAILKKISKADKIFTQKLGNVSAAKIEDCQRVLDFI
                     KNNQPPLIGFFLKKEKAEELFKNEPPRKILSYLGYQSVEEMLKNEDFDEVFAALRFVE
                     DGDWLNGVFFKQYEGLTVQDFEEREIKLKALSEKWRPAAEKFVTKKYHNISHLKEFGV
                     IFIIPVALDIPGEILRMISLIFHYYYEVKFYSDIFKQYSLDDNFSQKLITLLQGDLGG
                     RLSDDPRVCEWLIIQQYLAKNDKNDPRLFVPHINPEAIHWYKAGIDLVNLGHLLEGIE
                     EDFSFWQNLDWVGDYFQNERNEEELVSFNMIDNVMSLATGKERVKYLYHHQEALWNEI
                     FVRYLGREKLEEVLRQNLIKGVVRL"
     gene            3335..3589
                     /locus_tag="PL075_02080"
     CDS             3335..3589
                     /locus_tag="PL075_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97005.1"
                     /translation="MKLDYFEKKLNPKTYQTWKTILAYVALLLAQYLVELCLLILAIA
                     YWPAADYWLNERSHLQLIFSLSGIISLLLLLEAVNEGKRK"
     gene            3862..4527
                     /locus_tag="PL075_02085"
     CDS             3862..4527
                     /locus_tag="PL075_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOD97006.1"
                     /translation="MQLKAILRDTKKNPKQYLKEGYVPAELYGKGIENIHVAVNQKEF
                     TDVLKAAGESTIIELVLERENKKEEVHPVLIYETQRHYLSHQPIHIDFYQVQKGQKIK
                     THIPIEFVGESPAVKNLGGVLVKNMDEVEVEALPQDLPRSFIVDITKLDAIDSKICIK
                     DLNLPATIKTSAAPETAIVLVVPPREEEVVSAPAESISEIKIETEEKKAERKEEKEGE
                     SQS"
     gene            4541..5968
                     /locus_tag="PL075_02090"
     CDS             4541..5968
                     /locus_tag="PL075_02090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOD97007.1"
                     /translation="MSKNLGKPKIVADVLEKGSQTVFQKLPDPLGRSFGYRRHFPKIF
                     QNILVLIVLIIFVVSNTTLISLAPVALSQVSATEWQTLEQELQKIEAEIDAYQSNIIS
                     LQTQAKTLNQSIKLLDAQINKLNLQIKATNLQLTKLDNQIDTLRVSVTKTQNNIDMTQ
                     DMLVHALRQIYEEGQRSPFIVFLSENTLSEAFSAVNDLTSLEIEIKKSLDELVNLKSN
                     LIAQREDLSIQRQEALELKSIQEAQKKELEAKKKEKNDLVRLTKGQESKYQELLKKSQ
                     QTAAQIKSRIFTLLGGGEMSFEEAYKLAKFASEQTGVRPAFLLAILDRESSFGRNVGK
                     CNWKTAMHPTRDKPTFLKITQELGINPDSVFVSCPNADGVYGGAMGPAQFIPSTWWMY
                     KDRVAAITGNNPPSPWRNVDAFVAMALLLKDLGADKQTYAAEREAAARYYAGSRWRNH
                     LYGYGQWVADKAQKFEADIRNSNLG"
     gene            complement(5989..6732)
                     /locus_tag="PL075_02095"
     CDS             complement(5989..6732)
                     /locus_tag="PL075_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOD97008.1"
                     /translation="MSDVNSVILEIRAGVGGEEAALFAAELLRMYQRYAQKRGWQSSL
                     LDINYSSLGGVREAVLEITGEGVEILKNEGGVHRVQRIPATEKSGRVHTSTISVAVMS
                     RPKEAKIDIKPEDLEITFFRSSGPGGQNVNKVETAVRALHKPSGLIVSCQSERSQFKN
                     REKAIEMLKTKIYEAKWSADQGKISEERRQQIGTADRSEKIKTYNFPQDRLTDHRYKK
                     NFHNLEKIMDGDLDEVLKIYQSTPSSDNK"
     gene            complement(6741..7022)
                     /gene="rpmE"
                     /locus_tag="PL075_02100"
     CDS             complement(6741..7022)
                     /gene="rpmE"
                     /locus_tag="PL075_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOD97009.1"
                     /translation="MKKEIHPKYYPKAKVKCACGNVFEIGATVPEMQIEICSACHPFY
                     TGKEKIIDAAGRVDKFKKRLQKHSELAKQVKPKTAKKIVKKTKQSKEAK"
     gene            7306..8010
                     /gene="rpsB"
                     /locus_tag="PL075_02105"
     CDS             7306..8010
                     /gene="rpsB"
                     /locus_tag="PL075_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOD97010.1"
                     /translation="MSEENIQTTSIAIDIDRERILREMNEARVCFGRSTSRLHPKMKS
                     YIIGAKNTQTIIDLEKTYEMLIKAKEFFQSLLKEHKTILFVNINPATNDITLEAAQKT
                     NNPYIINRWVGGFLTNFSEISKRIKYYNELIQKNASGELEKYTKKEQLKFKKELEDLA
                     DLFSGVKDLSQLPDAIFVIGAKFHETAIKEAQKMHIPIIALANIDTDIEGIDYPIVGS
                     DLHRSSVEYITNFLIS"
     gene            8082..8663
                     /locus_tag="PL075_02110"
     CDS             8082..8663
                     /locus_tag="PL075_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOD97011.1"
                     /translation="MDLKQVKELRDLTGASVMECRNALEEAKGDFQKAQEILKKKGQA
                     IADKKESRETKAGLIEAYVHANGRIGVLVDLRCETDFVAKNPLFKELAHDIALQIAAM
                     APLYVSPADIPEEVVNKEKETYSAEFKDSKKPEKIINQIIEGKLEKWYGEVCLTKQPF
                     IKDQDQIVEDIIKAYIAKIGENIKIYRFIRFEI"
     gene            8667..>8893
                     /locus_tag="PL075_02115"
     CDS             8667..>8893
                     /locus_tag="PL075_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97012.1"
                     /translation="MLAIISQLFFFLGLVIIILLVARKTPKVVKEIDSKEEPLEIQIE
                     NHIGRSFFAIPWDKIDLATERFLEKTMRRLH"
BASE COUNT         2948 a         1403 c         1818 g         2724 t
ORIGIN      
        1 acaggatatt ctattgttga ggccaaaaaa acaacaatga atttatctgc tgataagcgt
       61 gtaattaata aggttggcga acccgtaaca ttaacctggg cagtggaaaa tagtttatta
      121 agcacaatag gcggtggcgg aggtggtcgt gctgaccgca tggaagaagc tgtggacgaa
      181 ggaggttgta attcggtgtg ggatattata aatgcagaat gtagcccttt agattttaat
      241 aaaatcactc cacaaaataa ttttgctaat tcttttatta acctctctaa taatttattt
      301 gcaaaaatta cgtcgatatt tatgcaaccg acgattgctc aaactactcc tcagcctgtt
      361 gtttttcttt ttgtcggcaa tgctgcttgt catccagaag gcgatgcaag tgattgtgat
      421 tttgccccca atagttatta tccagtttta accaatagcg gttcccttac tgtttatccg
      481 cagtcagata ccaattttta tttaattgtt tatggtgttt ctggtgaagg gcctaaattt
      541 tttgtttctg aaccactaga agtttctata gaagaagatg agcaagaaga aggcagggtt
      601 gatttaaaag cgaaggaaga ttcatcggga catgattata cggatgggcc gataacaatc
      661 agccgcggag ctgatattag tttgaaatgg agtggagaag aaaatctgga ttcttgtaca
      721 gccacggctt cgccagaaaa ttcagcttgg caaggcaaag tagcaacaac tggaaccaaa
      781 acaatttacg gcgtgacaaa ttcagtaatt tttaaactcg attgtactct taaaaatggt
      841 aattctgttt ctgattcagt gacggtaaat gtcgttgagc ctccagcttt agggacaatt
      901 gaggttaaag cccgcttgtt caatggttcc aatgtatcag actatacagg cccagtgaaa
      961 tatacaataa ctggccctca atcattgaca ggcgagacgc ctcaatcatt ttctgtttca
     1021 gccggaactt acactttgac ttatgtgagc ggtggcccta atgatgcagt ttgtattact
     1081 catcctcaga ctaaagttgt aaccgctcga gaaactacta ctttctattt agattttgaa
     1141 gtgcagaatt tatgttcgat cagcgttgat tatcaagtgc cgggatcaac agctgaatgg
     1201 gatgggaaag cgtcgtatgt tttaattggt ccgacaattt taacagggaa ttctttgccg
     1261 tacagcttta accaaattcc gccgggcgtc tattatattt cttatatcag tggcgcgcca
     1321 gagaatttaa tttttagtgg tgttcttgaa ggtaatggac aagtttgtta tcccaaagag
     1381 ccattgcaat ttactttgca atttgctgag ccgggggcaa caccaccggt gccattaccg
     1441 tcacctgaaa taggcgataa tccgccctct gctaaaattg gttgcgcagc tgataattca
     1501 attagttgta cagggaccat tgatggtgat gccaatacgc caactattac tttgtacaat
     1561 gcttctactg atccagatag agatataaaa agttgcacgt ggattattca aaaaacagga
     1621 ggagaaccaa tctcaaaaaa tacctgtcag cccgttagta tttatgatga cactggttct
     1681 tatggcgtca ctctaacagt gacagatagt cgaggcaata cagatagcgc tcaacttacg
     1741 gcaacagtta aaaatgtcac agaattaata gctaaattcg tttgggatcc tcaaattcct
     1801 acggtgggaa aggaggtgaa gttttatgat cgttctttga cgccgtcagg aactagtctt
     1861 aaaacctggc tttggagttt tgaagatggt tcgccagcta cttcgacaag tccaaaccca
     1921 gaagtaattt ttggcgcaac tggccctaag gcggttactt tgactgttac taataatgtt
     1981 aataacaagg cagtgattac gcagattatt gatgttcgaa atatcgcgcc tcagtggaaa
     2041 gaagtgattc ctaaatagat ctaaatttat tgggatatgc aaattgcagc gcaggaaagg
     2101 atagcaaaga ttttgcgttg tccttctcaa acaatttttg aattagagaa aaaaatggaa
     2161 gctatttctg gaaagaaagg cgtatttgaa gcacttgtgc aggaaaatag tgaaatcatt
     2221 gcagatcggt taaaaaattt agcggttaaa aatcaaactg ccgaagaaat ttctcacgca
     2281 attctgaaaa agattagcaa agcagataaa attttcactc aaaaactagg taacgtttct
     2341 gcagccaaaa ttgaagattg tcaacgagtg cttgatttta ttaaaaataa tcagccgcca
     2401 cttattggtt tttttctaaa aaaagaaaaa gcggaggaat tatttaaaaa cgaaccgcca
     2461 agaaaaattt taagttattt aggatatcag tcggttgaag aaatgcttaa aaacgaagat
     2521 tttgatgaag tttttgcagc gttgcgtttt gtggaagatg gcgactggtt gaatggcgtt
     2581 tttttcaagc agtacgaagg gttaacggtt caagattttg aagagcggga gattaaatta
     2641 aaagccttgt cagaaaaatg gcggccagca gctgaaaaat ttgtaacgaa aaaataccat
     2701 aacattagcc atctaaaaga atttggtgtt atttttatta ttcctgtagc attagatatt
     2761 ccgggggaaa tcctgcgaat gatttcatta atttttcact attattatga agtgaaattt
     2821 tattctgata tttttaagca atacagcctt gatgataatt tttcccaaaa attaattact
     2881 cttttgcaag gagatttagg aggaagatta agcgatgatc cacgtgtttg tgagtggtta
     2941 atcattcagc aatatttggc gaaaaacgat aagaatgatc ctagattatt tgtgcctcat
     3001 attaatcccg aagcaattca ttggtataaa gcaggtattg atctggtaaa tcttggacat
     3061 ttacttgaag ggattgaaga agattttagt ttttggcaga atttggattg ggtaggtgat
     3121 tattttcaaa acgaaagaaa tgaagaagaa ttagtgagtt ttaatatgat tgataatgtg
     3181 atgtcgttag cgacggggaa agagcgagtt aaatatttgt atcatcatca agaagcatta
     3241 tggaatgaaa tttttgtgcg ctatttgggc agagaaaaat tagaggaggt tttaaggcaa
     3301 aatcttatca aaggagtagt tcgattatga ttatatgaaa ttagattatt ttgagaaaaa
     3361 attgaatcca aaaacttatc aaacatggaa gacgattttg gcttatgtgg ctttgctttt
     3421 agcgcaatat ttagttgagt tgtgtctttt aattttagcc attgcttatt ggccagcagc
     3481 ggactattgg ttaaacgagc gcagtcactt gcaattgata tttagcctta gtggtattat
     3541 tagtttactg cttctgttgg aggctgtgaa tgaaggaaaa agaaaataaa aacaagagga
     3601 ataaaaattt taaatcttct attaagttga ttattggcaa gcgttgtttt ttgtagttaa
     3661 accaaataat gtagctcatg aattaattca tgagctattt ttaagatatc gttggcgagc
     3721 tttcaaagca attgctttga aagctcgcca acgaagtatc cttaatgccg ccacctattt
     3781 tttaatttat ttttgcatag actagcataa ccactacaac cagttgacga cagcaaagtt
     3841 ttttgttaat atgggtttac tatgcaatta aaagccattc ttagagatac aaagaaaaac
     3901 ccaaaacaat atttgaagga ggggtatgtc ccagccgaac tttatggtaa gggcatagaa
     3961 aatattcacg tggcagtgaa tcaaaaagaa ttcacagatg ttttgaaggc agcgggcgaa
     4021 tcaacaatta ttgagttggt gttagaaagg gagaataaaa aagaagaagt gcacccagtt
     4081 ttaatctacg aaacccaacg ccactattta tctcatcagc ctattcatat tgatttttat
     4141 caagttcaga agggacagaa aatcaaaact catattccta tagaatttgt gggagagtcg
     4201 ccagcagtta aaaatttagg cggtgtattg gtgaaaaaca tggacgaagt tgaagtggag
     4261 gcattacctc aggatttgcc ccgcagtttt attgtagata tcacgaaatt agatgcgatt
     4321 gattcaaaaa tttgcataaa agatcttaat ttgccagcaa cgattaaaac ttcagcagcg
     4381 cccgaaaccg ccattgtttt ggtggtgccg cctcgagagg aagaagtggt ttctgcacca
     4441 gccgaaagca ttagcgaaat taaaattgaa actgaagaaa agaaggcaga aagaaaagaa
     4501 gaaaaagaag gcgaatccca gagctaattc tctaaaagtc atgagtaaaa atttgggaaa
     4561 acctaaaatt gttgccgacg ttttagagaa ggggagtcaa accgtttttc aaaaactgcc
     4621 cgatccactc ggacgttctt tcggttatcg ccgtcatttt cctaaaattt ttcaaaacat
     4681 tttagttctt atagttttaa ttatttttgt tgtttctaat acaacattaa tttcattagc
     4741 accagtggcg ttgtcacagg tatcggctac agaatggcag actcttgaac aggaattaca
     4801 gaaaattgaa gctgaaatag atgcttatca gagcaatatt attagtttac aaactcaagc
     4861 aaaaacactc aatcaaagca tcaagctttt agatgctcaa attaataaac taaacttaca
     4921 aattaaagct actaatttac aactaacaaa attagataat caaattgata ctttacgagt
     4981 gagtgtgacg aaaacgcaaa acaatattga tatgactcaa gacatgctag ttcacgcctt
     5041 gaggcagatt tacgaagaag gccaaagaag tccattcatt gtatttttaa gcgaaaatac
     5101 tttgtctgag gctttttcgg cagttaatga tttaaccagt ttggaaatag aaattaaaaa
     5161 aagtttagat gagctggtta atctaaaaag taatttgata gctcaacgag aagatttgtc
     5221 tattcaacgc caagaagctt tagaattaaa aagcattcaa gaggctcaaa agaaagaatt
     5281 agaagcaaaa aagaaagaga agaacgatct agtgcgttta acaaaaggac aagaaagtaa
     5341 gtatcaagaa ttacttaaaa aatctcaaca aactgcggcc caaattaaaa gtcgcatttt
     5401 tactttgttg ggcggaggcg aaatgagctt tgaagaggct tataaattag caaagtttgc
     5461 ttctgagcaa accggagtaa gaccagcgtt tttgttggca attttagatc gagaatcttc
     5521 ttttggaaga aacgtgggaa agtgcaattg gaaaaccgcg atgcatccaa cgcgtgataa
     5581 accgacattt ttgaaaatta ctcaggaatt aggaatcaat cctgatagtg tttttgtatc
     5641 ttgtcctaat gccgatggtg tttatggagg agcgatgggg ccggctcaat ttattccttc
     5701 aacatggtgg atgtataaag atcgggtggc agcgattacg ggtaataatc cgccatcgcc
     5761 gtggcgcaat gttgatgctt tcgtggccat ggctttatta ttaaaagact tgggagcaga
     5821 taaacaaact tatgcagctg aacgagaagc agctgctcgc tattatgcag ggagccgatg
     5881 gcgcaatcat ctttatggtt atggtcaatg ggtagcagat aaagcccaaa aatttgaagc
     5941 agatatccgc aattcaaatt tgggctagtt tttatgacgc ttaaaaaatt atttattatc
     6001 agaagacgga gtgctttgat aaatttttag tacttcatcc aaatcgccgt ccataatttt
     6061 ttctaaatta tggaaatttt ttttataacg atgatctgtt aaacgatctt gaggaaagtt
     6121 gtacgttttt attttttcag agcgatcagc agtgccaatt tgttgacggc gttcctcaga
     6181 aattttgcct tgatcagcgc tccatttagc ttcgtaaatc tttgttttta gcatttcaat
     6241 tgctttttcg cggtttttaa attgtgagcg ttcgctctgg caagaaacaa ttaaaccact
     6301 gggtttatgg agagcgcgaa cagctgtttc tactttatta acattttgac caccagggcc
     6361 agaagaacga aagaaagtaa tttccaaatc ttcgggttta atgtcaattt ttgcttcttt
     6421 gggtcgcgac atcacagcta ctgaaatggt tgaagtgtga actcggcctg atttttctgt
     6481 ggccggaatg cgttgcactc gatgcacgcc tccttcattt tttaaaattt ccactccttc
     6541 gccagtgatt tccaaaacag cttctctaac gccaccgagg gagctgtaat taatatctaa
     6601 aagagagctt tgccagccac gcttttgagc atatctttga tacatacgta ataattcggc
     6661 agcgaaaaga gcggcctctt cgccgcccac gccagctcta atttccaaaa taacgctatt
     6721 aacatcagac attttatcta ttatttagct tctttagatt gtttggtttt tttgactatt
     6781 tttttggctg tttttggttt gacttgtttg gctaattcag aatgtttttg aagacgtttt
     6841 ttgaatttat ccactcggcc ggctgcatca ataatttttt ctttaccggt atagaaagga
     6901 tgacaggcag aacaaatttc gatttgcatt tctggtacag tagcaccaat ttcaaacaca
     6961 ttaccacagg cacattttac tttagcttta ggatagtatt taggatgaat ttcttttttc
     7021 atataaatca attttaaaga aaaaaacgga aaaagcaaga ggaatagaaa taaaaattaa
     7081 aagtaagggg gtataaaaat aagatgatat gagtgggaaa caagatgttt gttagagagt
     7141 gtggcggcgg aaaatttcaa agaaattttc cgccgccaca cgcaactttt ttcagtagta
     7201 tattaatcaa agatttttat tgtgattatt gttaaacaag caagaggcca aaattttttt
     7261 attctctctt gcaatttttt taaaaaataa taaaatttga atattatgag tgaggaaaat
     7321 attcaaacaa ccagtatagc aattgatata gatcgagagc gcattttaag agagatgaac
     7381 gaagcgaggg tatgttttgg acgaagcaca tcgcgccttc atcccaaaat gaaatcttac
     7441 attattggcg ctaaaaacac ccaaacaatc attgatttag agaaaactta tgaaatgctt
     7501 attaaagcta aagaattttt tcagagctta ttgaaagaac ataagacgat cctttttgtt
     7561 aatattaatc cggccacaaa tgacattact ttggaagcag ctcaaaaaac caataatccg
     7621 tatattatta atcgttgggt aggtgggttt ttaactaatt tttccgagat ttctaaaaga
     7681 attaaatatt ataatgaatt aattcaaaaa aatgcttcgg gagaacttga gaaatatacc
     7741 aaaaaagaac aattaaaatt taaaaaagaa ttggaagatt tggcagatct tttttctggc
     7801 gtgaaagatt tatcccaatt gccggatgct atttttgtga taggggcaaa atttcacgaa
     7861 acagccatta aagaagctca aaaaatgcac attcccatta ttgcgcttgc aaacattgat
     7921 actgatattg aaggaattga ttatccaata gtaggaagcg atcttcatcg ttcaagcgta
     7981 gaatacatca ctaatttttt aattagttaa aataattatt ttggcattac gattattttc
     8041 attaatataa taaacaataa gtcgatttgt ctatttgaaa tatggattta aaacaagtta
     8101 aagaattaag agatttaaca ggcgcgtccg ttatggagtg tcgcaatgcg cttgaagagg
     8161 cgaagggtga ttttcaaaaa gcgcaggaaa ttttaaagaa aaaaggacaa gcgattgctg
     8221 acaaaaaaga aagtcgagaa actaaagccg gccttattga ggcctatgtg cacgctaatg
     8281 ggaggattgg tgttttggtt gatttgaggt gtgaaactga ttttgttgct aaaaatccct
     8341 tattcaaaga attagctcac gatattgctt tgcaaattgc ggctatggct ccactatacg
     8401 ttagtccagc cgatattcct gaagaggttg ttaataaaga aaaagaaact tattctgccg
     8461 aatttaaaga tagtaaaaag ccggagaaaa ttattaatca gattatagaa ggtaagttag
     8521 aaaagtggta tggagaagtt tgtttaacca aacagccatt tattaaagat caggatcaaa
     8581 tagttgaaga tattattaaa gcttatatag cgaaaattgg cgaaaatata aaaatttatc
     8641 gttttattag atttgagatt taagttatgt tagccattat ctcacaatta ttttttttcc
     8701 ttgggctggt tattatcatt cttttagtag cgaggaaaac acctaaagtg gttaaggaaa
     8761 ttgatagtaa agaagagccg ctggagatcc aaatagaaaa tcatataggg cgaagttttt
     8821 ttgcaatacc atgggataaa attgatttag ctaccgagcg ttttttagaa aaaacaatgc
     8881 gtcgccttca cat
//
LOCUS       DAOSMC010000017         8193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_9769_length_8193_cov_16.028508, whole genome shotgun sequence.
ACCESSION   DAOSMC010000017 DAOSMC010000000
VERSION     DAOSMC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9769_length_8193_cov_16.028508"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1560)
                     /locus_tag="PL075_02120"
     CDS             complement(217..1560)
                     /locus_tag="PL075_02120"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOD97013.1"
                     /translation="MDKETNDIMDKIISLAKRRGFIFPSSEIYGGFSSSYDYGPLGVE
                     LKNNIKHLWWKEMLQYHDNIVGLDGAILMSPKVWQASGHLSAGFADLLVECSKCHHRF
                     REDAIQNNRCPDCGGTLMPPRKFNLMMKTFVGPLEDEASVTYLRAETCQSIYLNFENV
                     LNTMRLKIPFGIAQIGKSFRNEINPGNFTYRTREFEQMEMQFFVRPDEAEKWFNYWVE
                     QRLNWYINLGIKKENLRLHQQTKEELAHYARMAVDIEYKFPFGWKEIEGIHNRGDWDL
                     SNHSKYSGHELVYFDEKTKEKYTPYIIETSAGADRGTLVFLIDAYEEVKGGRSQTTES
                     KKEVEIVLKFHPSIAPVKAAVFPLLKNKENLVTLAKEIYQNLKKHFIVQYDEVDSIGR
                     RYRRQDEIGTPYCITVDFQSLEDKSVTVRDRDTLNQERVFIDDLLPYLKKKLEFE"
     gene            complement(1572..3482)
                     /locus_tag="PL075_02125"
     CDS             complement(1572..3482)
                     /locus_tag="PL075_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97014.1"
                     /translation="MALEDVEKRLYKMKEENPLESEEEEHLGSPLPEENRPSKKEPSP
                     SPKKTKERPPINFYQKSKKSNIVLYIIIAILFVGLVVEGYFLAQKVSTQKTGIQLSIK
                     AQENILLGEAFDLEVAYKNDSANLLQDADLIIELPDDIQVLGEDNNTKLLKKSLGDLG
                     TGTSNVEKYTLVAMGTPNRIASIKTTLQYGIVGFSGRYEKSQSQTITIGGPIIDFNLS
                     VPQKIISGEEFSFKINFVNNSIKSLSNIRIQLFYPLGFNYTGASIEPVDGNNMWIWGM
                     LNPQESAEIVIRGTIVGEAGSFYEMGVNMSLISENQIISLDKKTAMVSILESPLNLSI
                     YVNNSKDYIAKNAEVLEYRIDYENNTDIALSEVIIKANLEGQMFNLSSLLTNGYFDSL
                     KKQIIWNAGNTPQLKLLNKGDKGSVSFKIYTASDYPVRSFNDKNLSLKVKATIESPSV
                     PPNSNLKNTTAIAELTTKVVSKVELQSYALFRDAKSGIVNKGTLPLKVGKSTNFTIHW
                     KLINYNNDLTDVSIKTTLPQGIGWTGVIAGNYGDLEPQYNERTSELVWNIKDVPAFTG
                     ILLPAKELIFQINATPSLNQVGRYMELISEGTLTATDVFTGQEIKIKIPALNSQLSAD
                     PTVEKEEGMVQL"
     gene            complement(3493..4125)
                     /gene="recO"
                     /locus_tag="PL075_02130"
     CDS             complement(3493..4125)
                     /gene="recO"
                     /locus_tag="PL075_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOD97015.1"
                     /translation="MIEYNTQAIVLDKQIINEADAQVVLYTELLGKVELIAKGAKKIT
                     AKLNSHLEILNLIESTIITVNSKHLTSALTINRFSNIRHQEKILENAIKMVKFFNQVI
                     VASEKDEFLWQSLCNYLSELDRITMENEKSIMLMNLINIDFLIKLETALGLMPAAEEQ
                     LHYFQQPTIEMIKLISERKTQELIESGNLLELKKIDYNKIEQDLKEKMIL"
     gene            4207..4294
                     /locus_tag="PL075_02135"
     tRNA            4207..4294
                     /locus_tag="PL075_02135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4241..4243,aa:Ser,seq:gct)
     gene            4472..4924
                     /locus_tag="PL075_02140"
     CDS             4472..4924
                     /locus_tag="PL075_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97016.1"
                     /translation="MDKKFLYILIVLILVIVGLYFLLGNKGVQKETEIASPTPIQTEQ
                     PSPTPTAADQTMVDCGKAENPACFIGRANSCLPVKAQMVGSDNTTQIEIMVLGVEDGK
                     CHFQRKINGIMDLNCYFPKGTNIMNAIDQTFGNDHGLQEVLDAACANW"
     gene            complement(5041..5256)
                     /locus_tag="PL075_02145"
     CDS             complement(5041..5256)
                     /locus_tag="PL075_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97017.1"
                     /translation="MQQIEIVSKFIKNFVWGAVLEGILIILTAILILIYPRFLNILVA
                     CILIASAIVCFIIAAKAHRYSKIEVEL"
     gene            5377..6354
                     /locus_tag="PL075_02150"
     CDS             5377..6354
                     /locus_tag="PL075_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97018.1"
                     /translation="MIWLYFVIFIIASVVLYIACETLVKIIIRVSGFLNVREFVIAFF
                     VMALATALPNLFLGISSAVNQISQLSFGDVLGNNFIALTLAVSLGILFSKNKEIPTES
                     RVVQTTSIFTFIAAFLPLLLAIDGKISRGDGLILIIAFIAYAYWLFSKQERFRKIIPS
                     QSIEKPVQAVPQFKNFIKDIFVALALIVVIFIAAQGIIFAAQSISTQLSIPLIMVGLL
                     ITGLANALPEIYFSINSARRGETWMMLGNLMGSIIFPATLVIGIVSLIQPIIITDFSS
                     IALARFFLIVALVFFFIFSQTDKKLSKKEAFFLLTLYILYCIIEIIKLA"
     gene            6405..7040
                     /locus_tag="PL075_02155"
     CDS             6405..7040
                     /locus_tag="PL075_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOD97019.1"
                     /translation="MDRIEFLTNFIYESERIAQLCPNYIEIKESLKNNKQIGHVGAIL
                     YLEKRSKEKELVLTEEDVKIVHELIFNEVHRRIKMTFSESERGGMYEEHKNLLGVLIG
                     TPASLIPQKMEKLISVLNKHQNAFIQRKQMVDLEFISNFHYHFALISPFSSCNELVNR
                     ALLLYLLWYFEKTPFIIPMKEREKYLMAFQTKNPQALSEYFEKKELEISKN"
     gene            complement(7089..7205)
                     /gene="rrf"
                     /locus_tag="PL075_02160"
     rRNA            complement(7089..7205)
                     /gene="rrf"
                     /locus_tag="PL075_02160"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(7249..>8193)
                     /locus_tag="PL075_02165"
     rRNA            complement(7249..>8193)
                     /locus_tag="PL075_02165"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2538 a         1505 c         1276 g         2874 t
ORIGIN      
        1 gctgtaattt tatattcaac aattttacct ttttccaaag aaaccgattg gcttagaatt
       61 tttatccctg ttaaattata tttgtctttt ccaacaaatt caataatgcg atttaaagcg
      121 tcttcaaagc tattgggaga agtcccgata atttcacgaa ttttgactat agccattttt
      181 taaatttttt aaccgacctt taatctaaaa tccgaattat tcaaactcta gtttcttttt
      241 taaatacggc agaagatcgt caataaaaac tctctcttga tttaaagtat cacgatcacg
      301 caccgtaacg gatttatctt ctaaactttg gaaatcaaca gtaatacaat aaggagttcc
      361 gatctcgtct tgacgacgat agcgtctacc aattgaatca acttcatcat actgaacaat
      421 aaaatgtttt ttaaggtttt gataaatttc tttggctaaa gttaccaaat tctctttatt
      481 ttttaataat gggaaaacgg ctgctttaac aggcgcaata gaaggatgaa atttcaaaac
      541 tatttccact tcttttttag attcggttgt ttgactacgc ccgcccttga cctcttcgta
      601 tgcatcaatc aaaaacacca atgttccgcg atcagctccg gcagatgttt cgataatata
      661 aggagtatac ttttctttgg ttttttcatc aaaataaacc agttcgtgac ctgaatattt
      721 tgaatgattt gataaatccc aatcgcctcg attatggatt ccttcaattt ctttccaacc
      781 aaaaggaaac ttgtattcaa tatcaacggc catccgagca taatgagcta attcttcttt
      841 tgtttgttga tgaaggcgaa ggttctcttt tttgatgcct aagttaatat accaatttaa
      901 acgttgttca acccaataat taaaccactt ttccgcttca tctggtcgaa caaaaaattg
      961 catttccatt tgttcaaact cgcgggtacg ataagtaaaa ttaccgggat tgatttcatt
     1021 acgaaaactt ttgccaattt gggcaattcc aaaaggaatt tttaaacgca tcgtattcaa
     1081 aacattttca aaatttaagt aaatactctg acacgtttcg gccctcaaat aagtcaccga
     1141 ggcttcgtcc tcaagtggtc ctacaaaagt tttcatcata agattgaatt ttcttggcgg
     1201 cattaatgtt ccaccgcaat cagggcaccg attattttga atagcatctt ctctaaaacg
     1261 atgatggcat tttgaacatt caaccagcaa atcagcaaac cctgctgata aatgccccga
     1321 tgcttgccaa acttttggag acattagaat ggcgccatca agaccaacaa tattatcatg
     1381 atattgaagc atttctttcc accaaagatg tttaatatta ttctttaatt ctacccctaa
     1441 ggggccgtaa tcataagaac tagaaaaacc gccataaatt tctgaagatg gaaaaataaa
     1501 accacgtcgt ttagctaaac ttataatttt atccattata tcatttgttt ctttatccat
     1561 atttttatca tttatagctg taccattcct tcctcttttt caaccgtcgg atcagcgctt
     1621 aattgagagt ttaaagcagg tattttaatc tttatttctt gacctgtaaa gacatccgtg
     1681 gccgtaagcg ttccttcaga aataagctcc atgtacctgc ctacttgatt aagagaagga
     1741 gtggcattaa tttgaaaaat taattcttta gccggcaata aaatacccgt aaaagcaggc
     1801 acgtctttaa tgttccaaac taattcggat gttctttcat tatattgggg ctcgagatcg
     1861 ccataatttc cggcaattac cccagtccag ccaattcctt ggggcaacgt tgttttaatg
     1921 gaaacatcgg ttaaatcatt attataatta attaatttcc aatgaatagt aaaattagtg
     1981 ctcttgccta cttttagagg caatgttcct ttattaacga tacctgattt agcatctctg
     2041 aataaagcat aagattgcaa ttcaacttta ctaactactt tagtagttaa ttcggctatt
     2101 gctgttgtgt ttttaagatt actattcgga ggtacgctag gtgattcaat ggtagctttt
     2161 actttcaaag aaagattctt atcgttgaag ctgcgcaccg gataatcact agcagtatag
     2221 attttaaaac tcaccgaccc tttatctcct ttgttcagca attttaattg gggtgtattt
     2281 ccagcattcc aaataatctg tttttttaag gaatcaaaat aaccattagt caaaagactg
     2341 ctaaggttaa acatttgtcc ttcgagattg gccttaatta tcacctcact taaagcaatg
     2401 tctgtattat tctcgtaatc aattcgatac tcaagtactt ctgcattttt agcgatataa
     2461 tctttggagt tattaacata gatgctcaga ttgagcggcg attctaaaat acttaccatt
     2521 gctgttttct tgtctaaact aataatttga ttttccgaaa taagactcat attaactccc
     2581 atttcataaa aactgccagc ttctccaaca atagttccgc gaataacaat ttcggcggac
     2641 tcttgaggat tgagcatccc ccaaatccac atattgtttc catcaacagg ttcaatgctt
     2701 gcaccggtat aattaaagcc caaaggatag aataattgaa tacgaatgtt agaaagcgat
     2761 ttaatagaat tgttaacgaa attaattttg aaactgaatt cttctccgct aatgattttt
     2821 tgaggaacag aaagattaaa atcaataatc ggtccaccaa tagtaatagt ctgactttga
     2881 gatttctcat atcgcccgct aaagcccacg atgccgtatt gaagcgttgt tttaatgcta
     2941 gcaatgcgat taggcgtacc cattgccaca agggtgtatt tttcaacatt ggaagttccg
     3001 gtgcctaaat cacccaaact ctttttgagt aattttgtat tattatcctc gcctaaaact
     3061 tggatatcat ccggtaattc aataattaaa tcggcatcct gcagtaaatt agccgaatcg
     3121 tttttataag ctacttctaa atcaaaggct tcacctaaaa gaatattttc ttgcgcttta
     3181 atgctaagtt gaatgccggt tttttgagtc gaaactttct gagccaaaaa ataaccctct
     3241 accactagtc caacaaataa aatagcaata ataatataaa gaacgatgtt ggatttttta
     3301 cttttttgat aaaaattaat tggcggccgt tcttttgttt tttttggaga agggcttggc
     3361 tctttttttg atggtctatt ttcttcgggc aatggagatc ccaaatgctc ctcttcttct
     3421 gattcaagag gattttcttc tttcattttg taaagtcgtt tttctacatc ttctaatgcc
     3481 ataattttat atttataaaa tcattttttc ttttaaatct tgctctattt tattataatc
     3541 tatttttttt aattctaata gattgcccga ctctatcaat tcttgtgttt ttctttcaga
     3601 aattaatttg atcatttcta ttgtaggttg ctgaaagtaa tgcaactgct cttctgctgc
     3661 cggcattaaa cccaaggcgg tttctaactt tattaaaaaa tcgatattga ttaaattcat
     3721 caacattata cttttctcat tttccattgt tattctatct agctcgctaa gataattgca
     3781 caacgactgc catagaaatt catctttttc actagcaaca ataacttgat tgaaaaactt
     3841 aaccatctta atggcatttt caagaatttt ttcttgatgt cgaatattcg aaaaacgatt
     3901 aattgttaag gcgcttgtaa ggtgcttgct attaacagta ataatagtag attctattaa
     3961 attaagaatt tctaaatgac tgttcaattt agctgtaatt tttttagcac ctttagcaat
     4021 taattctact tttcctagca attcagtata aagaacaact tgagcatcag cttcattaat
     4081 aatttgttta tccaaaacaa tagcttgagt attatactcg atcatattct attattataa
     4141 atctgacacg gaaaatttgc aaaaaacctt cgttttgact ataattaaaa tattgaaatt
     4201 aaataaggaa gggtggccga gtggcttaag gcaaaggttt gctaaacctt ggcgctgtta
     4261 aggcgcctcg tgggttcgaa tcccacccct tccgctatta tttacttaaa gcaaaaatag
     4321 ccctcatttc tcgctaaaaa tcaggcaatt tttttaatta taaattttaa ttaatgtgta
     4381 attttttaat ctttgtagat attttaataa aaattgctta tatttaaata aaggtcgctg
     4441 ctaatgaaaa ttaacattac taaattaaaa tatggataaa aaatttttat atattcttat
     4501 tgttttgatt ttagttatcg tcggattata ttttctttta ggaaataaag gggtgcaaaa
     4561 agaaacagaa attgcatcac caacacctat tcaaacagaa caaccctctc ctactcctac
     4621 tgccgctgat caaacaatgg ttgattgtgg caaagcagaa aaccccgcct gttttatagg
     4681 cagagcaaat agttgtttgc cggtaaaagc tcaaatggtt ggttcagata ataccaccca
     4741 aattgaaata atggttttag gagtagaaga tggaaaatgt cattttcaga gaaagatcaa
     4801 tggcataatg gatttgaatt gttattttcc aaaaggcact aatatcatga atgctattga
     4861 tcaaaccttt ggaaatgatc atggcttaca agaagtactt gatgcggctt gcgcaaattg
     4921 gtaatttact aaaaaagaaa ataaatattt attatcaaac ctcttatttt atataataag
     4981 aggtttgatt ttttgtataa tttctaaata ttaatagaga tttatacaat gttttatttt
     5041 ttacaattcc acttctattt ttgaataacg gtgggcttta gcagcaataa taaaacacac
     5101 tatagctgat gcaattaaaa tacaagcgac taaaatattg agaaatctcg gataaattaa
     5161 tatcagaatg gctgttaaaa taattaagat gccctctaag actgcgcccc aaacaaaatt
     5221 tttgataaat ttagaaacaa tttctatttg ttgcataaat ttatttttta aagagtcgac
     5281 tttttcatac ttctattata ctaaaatatt ggctataatt aaagatggtt gaatgattta
     5341 tttgtataaa taaaattttt cggataaaat acttttatga tttggctata ttttgtaatt
     5401 tttattatcg cctcagttgt tctctatatt gcctgcgaaa cattagttaa aattattatt
     5461 agagtttccg ggtttttaaa tgtgcgcgaa tttgtaattg ccttttttgt aatggctttg
     5521 gctacggctt tgcctaattt atttttaggc atcagctccg ctgttaatca aatttctcaa
     5581 ctttcttttg gagatgtgct gggtaataat tttatagctt taactttagc cgtctcactc
     5641 ggtattttat ttagtaaaaa caaagaaatt cctaccgaaa gtcgagttgt tcaaacaacc
     5701 tccattttta cttttattgc cgcttttctg cctctactat tagctattga tggcaaaatt
     5761 tctcgtggtg atggcctgat acttattata gcttttatcg cttatgccta ttggctcttt
     5821 tccaaacaag aacgattccg aaaaattatc ccctctcaat caattgaaaa accagtacaa
     5881 gctgtccctc aattcaaaaa ttttataaaa gatatatttg ttgcactagc attaattgta
     5941 gttattttta ttgccgctca aggtattatt tttgctgctc aatcaatttc cactcaatta
     6001 agcattccat taattatggt tggtcttcta attaccggtt tggcaaatgc cttacccgaa
     6061 atttattttt ctatcaattc tgctcgccga ggcgaaacat ggatgatgct tggaaattta
     6121 atgggttcta ttatttttcc agctacttta gtaattggca ttgtttctct gatccagccc
     6181 atcattatta ccgatttttc ctcaattgcc ctggctcgat tctttttaat tgtcgcttta
     6241 gtatttttct ttattttttc ccaaacagac aaaaaactaa gcaaaaagga ggcttttttc
     6301 cttttaacgc tttacattct ttattgtata atagaaataa taaaacttgc ttagatttta
     6361 taacaaaatt tggttcttta aaaatttatt taaaggaggt ttaaatggat agaattgaat
     6421 ttttaacgaa cttcatttat gaatctgaac gaatcgcaca gctctgtcct aattatatag
     6481 agattaaaga atctttgaaa aataataaac aaattggtca tgttggcgct atcctttatt
     6541 tagaaaaacg aagcaaagaa aaagagttgg ttttaacaga agaagatgtt aaaattgttc
     6601 atgaattaat ttttaacgaa gttcatcgcc gaattaaaat gactttttct gaatctgaaa
     6661 gaggggggat gtatgaagaa cataaaaatc ttctcggcgt tttaattggc acgcctgcct
     6721 ctctcattcc acaaaaaatg gaaaaattaa taagtgtttt gaacaaacat caaaatgcat
     6781 ttattcaacg caaacaaatg gttgacctag aatttattag caactttcat tatcatttcg
     6841 ctttaattag tcccttctct tcgtgtaatg aactagtcaa tagagcttta ttactctatc
     6901 ttctgtggta tttcgaaaag actcctttta ttattccaat gaaagaaaga gaaaaatatt
     6961 taatggcttt tcaaactaaa aatccccagg cgctttctga atattttgaa aagaaagaat
     7021 tagaaattag caaaaattaa actaaaaaac ccttcagtag aagggttttt ttatttgtaa
     7081 tcaaagaatt ctcggcgaag cttactcttc ccaccgtgtt cgggtgagta ccattggcgt
     7141 ccgacgcttt cacttctctg ttcggaatgg gaagaggtgg ttcacgtcga actaaatcac
     7201 cgagaaatct ttgattataa atcagaattt atcaatgaag cattttttat cgggacgaat
     7261 cacccgatta gtacgtctcg gctcaaattc ttacgaatct tacacctgac gcctatcaac
     7321 ccctaattct tgcgaatcgg ggttccgaca tacgtcggac ctagtcatct tctagggggt
     7381 gaaatgacac ctaatcttag gatgggcttc gcgcttagat gctttcagcg cttatcccga
     7441 ccgaacatag ctacccgaca attctcctgg tggaaaagcc ggtacaccag aggttcgttc
     7501 aacccggtcc tctcgtacta gggtcgactt ccttcaagtg tcaacgcctg caacaggtag
     7561 agaccaacct gtctcacgca taataccaat ttatcttata aatattggtt ggtttgtcct
     7621 ttattactaa aggtatggac tataccatta ccatagaatt atttcataat tctttaggca
     7681 atcgacgtat tagctgcttt tgaaagcaac tctctctatc tctagagaag tctctacggg
     7741 gttaatatat gtctttttcg ttttttagaa taatttaatt tcttaaattt atcaattaaa
     7801 tctactacct ctctaaaatc attataattc ttatttttta gacatataca acttccctcg
     7861 gtattatccc aacatatgac aggttatttt gcaaataatg ttaggacttc accgatatga
     7921 gtcgattttt tcacttcgaa gtttccgacg aaggccgcca atttgacggt ttaaacccag
     7981 ctcgcgtatc cttttaattg gcgaacagcc aaacgcttgg gaccttctcc agccccgcgc
     8041 taggatgagc cgacatcgag gtgccgaacc agatcgtcga tagggactct cggatctgac
     8101 tagcctgtta tccccggggt agcttttgtc cgatgtctcc ggccgttcta aaaagaaccg
     8161 tcgggtcact aagttctgct ttcgcatctc aat
//
LOCUS       DAOSMC010000018         7466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_10994_length_7466_cov_19.915261, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000018 DAOSMC010000000
VERSION     DAOSMC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10994_length_7466_cov_19.915261"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="PL075_02170"
     CDS             <1..1914
                     /locus_tag="PL075_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOD97020.1"
                     /translation="QRTWLIIMGLVSLVVFLWVQTRGSIIGLIVGLFAALIVSIFTIP
                     NKIYKKFAMIGAGVIVLMGILFFVFQPQIQQSRISQEIPFVGRLASISLSDASTRARI
                     LNWQRSFNWWKERPIFGYGQDMFYQVFDKNYSGDDFALSRERFDRAHNKFFDVLLMNG
                     AVGFLAYLFLLGVVGYLIILKIKKCQTINDRFAWISIFGLFIAYLVHNFFVFDTPANS
                     IFFYFFLAFLNIETWDLQKRKMLSEEKKVADSKRVPIKSSITAAKFGSTQILILIIAL
                     LGASFVFYHVDAKPFKAASLMYQANQIRPNQVKDVFAVFQKAVNYNTFLNTEIKKPWA
                     DYFHSYLVYVQQGKIEANKEDVVWAYEQIKDNLLSGYAHEPMIDFYVYLANIAIRMSD
                     LSLFSSEEQADYWEEAEAFFEFIATNWPKRTDLYINAAFFAPDDATRQKWLDRIIAST
                     PKYAPALWLKGVSLIKQEGDAQEIYDMLSRALDNDYQFVFSNNSDFIFSAQDQLVNLK
                     KEGKLLQLIDQGIIEEEKILANQKLNAFEKNLHTQKLKSLVDLGILMEIINPNPTNED
                     LEHLITYLDKADALQKNRIEVLVKYAAAYAQLHNKEKAIEYASKVKEIDSRYATDVEV
                     FIKIVEEEKWDQL"
     gene            2107..2904
                     /locus_tag="PL075_02175"
     CDS             2107..2904
                     /locus_tag="PL075_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOD97021.1"
                     /translation="MEENQSEKIKKPAEGKKKDLVLPIAIVLAALIIGGAMVYSKGLS
                     TIESADSALNTSNSNGVKLEIASDQVFLGKADAPITIFEFGDYQCPYCRRFHMLAHLD
                     LVKDYVDSGLAKVVFMDFPLPGHEFAQKASEAAWCAKDQNKFWEMHDALFENSGTTNG
                     LTIDNIIKYAGNLGMDTQIFEQCLNSSKYAARVQQILEMSARLGIQATPTTIITNKPL
                     PLVLDAKAVVAAYQSSNGGIANLGDSFLIIGAQPYSTIKSVIDSLVK"
     gene            2904..3149
                     /locus_tag="PL075_02180"
     CDS             2904..3149
                     /locus_tag="PL075_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOD97022.1"
                     /translation="MIKIYSTPSCPYCHLAKDFFQQNNIEFEDFNVAEDEKALQEMVS
                     KSGQMGVPVIDIDGEIIVGFNRAEIERILKEKGLSKN"
     gene            3265..4581
                     /locus_tag="PL075_02185"
     CDS             3265..4581
                     /locus_tag="PL075_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97023.1"
                     /translation="MKKLGVYLSLPEMILFWIMLIVILFLMGVIFYFVAPPWKWIGIA
                     VGVIFLLISFFVNLRNTIKHYNILAEREQVRTIISHFTDAIVAYDPDFRITVFNASAE
                     NLLDLKASDVLNQKITPEWAKNSKTKLLTQVLFPSLAPAMSWKSDPAVFPNIMDLSFT
                     DPILELTVTTIRVTDSKGKILGFFKIIKDRTREEEILKTKSEFLTVAAHQMRTPLSGI
                     KWSLETLLGEGNENLTEMQRELLQKNLEATDKILRLANDLLDVANIESGRFGYEFVQA
                     DLMDLIQKSISDYLYLADQHKIKIYLEPPPDGLEPFKFDPMRIKIVIQNLLENALRYN
                     VENGQIIVKVEKKPPYVEVSVSDTGIGIPKDELPKLFTKFFRASNVLKYETEGTGLGL
                     YITKNIIEAHGGKIWAESIENRGSTFYFTLPMDEKLIPKRHISSVI"
     gene            4814..5002
                     /locus_tag="PL075_02190"
     CDS             4814..5002
                     /locus_tag="PL075_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97024.1"
                     /translation="MKFWLFSLIGVIFLLLFFIRPWGPEKEWLTIVTALGFYCWLGWL
                     FVQGLKEDKNKKRNKQSW"
     gene            5062..5883
                     /locus_tag="PL075_02195"
     CDS             5062..5883
                     /locus_tag="PL075_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97025.1"
                     /translation="MKILSVWERISDALQREWVDKSRKGLVEGVQVDRERKQLKAQCI
                     ISSQNEVVPVVRMDKRPDWFRISIRVPSWRKIVAVTEDRILYPIEKEGYKLLFFRNAT
                     VLEFCRGLLSATYYAVRAEGSRFFLIESSRFELGVPRFIETREGIRDFLVENHIPQDL
                     LEPALELIQKQKKDFSNPVWKEGLEKSKVKPSQKVEVPKEIRPAMKVPRQPEEKAPIE
                     VPMKTSDEPAKLRRSPKKRERRERDEKPRRLRFQMDGDDVEFRGVRGLSLKDLEK"
     assembly_gap    6174..6273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6274..>7466
                     /gene="ftsH"
                     /locus_tag="PL075_02200"
     CDS             <6274..>7466
                     /gene="ftsH"
                     /locus_tag="PL075_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOD97026.1"
                     /translation="FKDVADLDEAKQELMEVVEFLKNPKKFLDVGAKIPRGVLLMGPP
                     GSGKTLLARAVAGEAGVPFFHISGAEFVELFVGVGASRVRDAFTTAKRAAPSILFIDE
                     IDAVGRERGAGLGGGHDEREQTLNQILVEMDGFDRETNVIVMAATNRPDILDPALLRP
                     GRFDRRIVLDLPDLIGREEILKIHCRGKMLENGVDLRRVAERTPGFSGADLANLVNEA
                     AILAARRNKKEIGQSELLESIEKVILGPERKSRYISDKEKHIIAYHEAGHALVTYYSP
                     DADPVQKVSIIARGQAGGYTLKMPLEDRRLQTRKQFLAELAILLGGYTAEKLYLGDIS
                     TGAANDLKEASELARKLVMQFGMSEKLGPVTFGKTEEMIFLGREISTVRNYSEETAEK
                     IDKEVA"
BASE COUNT         2330 a         1119 c         1563 g         2354 t
ORIGIN      
        1 caaagaacct ggctcattat tatgggactt gtttctttgg ttgtttttct ttgggtacaa
       61 actcgaggtt caattattgg tttaattgtt ggtttattcg ctgctctcat tgtttctatt
      121 tttactattc ctaataaaat ttataagaaa tttgctatga ttggtgctgg tgtcattgtt
      181 ttgatgggca ttcttttttt tgtttttcaa ccgcaaattc agcagagtcg gattagccaa
      241 gaaattcctt tcgttgggcg gttagccagc atttctttat ctgacgcttc aacgcgagcg
      301 cgcattttaa attggcagcg aagctttaat tggtggaaag aacgtccaat ttttggttat
      361 ggtcaagata tgttttatca agtatttgat aaaaattata gcggtgatga ttttgctttg
      421 agtcgggagc gttttgatag agcccataat aaattttttg atgttttgct aatgaatggc
      481 gctgtgggtt ttttggctta tttatttttg cttggtgtcg ttggctatct gattattttg
      541 aaaattaaaa agtgtcaaac tattaatgac cgctttgctt ggatttctat ttttggctta
      601 tttattgctt atttagtcca caactttttt gtttttgaca caccggcgaa ttcaatcttc
      661 ttctattttt ttctagcttt tcttaatatt gaaacgtggg atttacagaa aaggaaaatg
      721 ttatctgagg aaaaaaaggt ggcagattca aaacgcgtgc ccattaaatc gtcaatcact
      781 gcagcgaagt ttggttctac acaaatatta attctcatca tcgctttgtt aggggctagt
      841 tttgtttttt atcatgttga tgcgaaaccc tttaaagctg cctctttaat gtatcaagct
      901 aatcaaattc ggcctaatca agtgaaagat gtgtttgctg tttttcaaaa agcagttaat
      961 tataacactt ttcttaatac tgaaattaaa aaaccctggg ctgattattt tcattcctat
     1021 cttgtttatg ttcaacaagg caaaatcgaa gccaacaaag aggatgttgt ttgggcgtac
     1081 gaacaaatta aagacaattt actttctggt tatgctcatg aaccaatgat tgatttttat
     1141 gtttatttag ccaatatcgc tatacgaatg tctgatttga gccttttttc atcagaagaa
     1201 caggcggatt attgggaaga agcggaagca ttttttgaat ttatagctac caattggccg
     1261 aagcgaacag atttatatat taatgccgca ttttttgcgc cagatgatgc tactcggcaa
     1321 aaatggcttg atcgtataat tgccagtact cccaagtatg cgccagcgct ctggcttaaa
     1381 ggagtttctc ttatcaagca ggagggtgat gctcaggaaa tttatgatat gttaagtcgg
     1441 gcattagata acgattatca atttgtattt agcaataatt ctgattttat tttttctgct
     1501 caagaccaat tggttaatct taaaaaagag ggtaagcttc tgcaattaat tgatcaagga
     1561 attattgagg aagaaaaaat tttggctaat caaaaattaa atgcctttga aaagaattta
     1621 catacccaaa agcttaaaag tttagttgat ttgggcattt tgatggaaat tatcaatccc
     1681 aatcctacca atgaagattt agagcattta attacctatt tagataaagc cgatgcgttg
     1741 caaaaaaatc gcattgaggt gttagtaaaa tacgcagcag cttatgctca attgcataat
     1801 aaagaaaaag ctattgaata tgccagtaaa gtaaaggaaa tagattctcg ttatgcgact
     1861 gatgtagaag tgtttatcaa gatcgttgaa gaagaaaagt gggatcagct gtaaaattaa
     1921 attttccatt tttgctattt ttagtaatat atataaggta taaatgatta gtgcggcgga
     1981 aaattttctt tgaaaatttt ccgccgcacc ccaaacaatt aaaaagttgc aattattgat
     2041 ttttttgttt aaaattaaaa ataagattaa agtcgttttt tattagagac atttttaact
     2101 ctcattatgg aagaaaatca atcagaaaaa ataaaaaaac cagcagaagg caaaaagaaa
     2161 gatcttgttt tgccaattgc tattgttttg gctgctttaa ttattggagg agcaatggtt
     2221 tatagcaagg gtttaagcac gattgagtca gctgatagcg cgcttaatac ttcaaattca
     2281 aatggcgtta aattagaaat tgctagcgat caagtttttt taggcaaagc tgatgcgccc
     2341 attaccattt ttgaatttgg cgattatcaa tgcccctatt gcaggcgttt tcatatgctt
     2401 gcccatcttg atttagtgaa agattatgtt gattcaggat tagccaaggt ggtctttatg
     2461 gattttcctt tgccggggca tgagtttgct caaaaggcat ctgaagctgc ttggtgtgca
     2521 aaagatcaaa ataaattttg ggaaatgcac gatgcgcttt ttgagaattc aggcacaact
     2581 aatggtttaa cgatagataa tataattaaa tatgcaggga atttaggcat ggacacacaa
     2641 atttttgagc agtgtttaaa ctcaagcaaa tatgctgctc gcgttcaaca gattttagaa
     2701 atgagtgctc gcttgggcat tcaggcaacg ccaacaacaa ttattactaa caaaccattg
     2761 ccgttagttc ttgatgccaa ggcagtagta gctgcttatc aaagcagcaa tggcgggata
     2821 gcgaatcttg gtgacagctt tttaattatt ggcgctcaac cttacagcac tattaagtcg
     2881 gtaatagatt cattagtaaa ataatgatta aaatttattc aacccccagt tgtccttatt
     2941 gtcatttagc caaagatttt ttccaacaaa acaatattga atttgaagat tttaatgttg
     3001 ctgaagatga aaaggcctta caggaaatgg ttagtaaaag cggccaaatg ggcgtgccgg
     3061 taattgatat tgatggagaa attattgtgg gatttaatcg ggcagaaata gaaagaattt
     3121 tgaaagagaa agggttaagt aaaaattaag aaatattgtt agaaaatcta aatcagttgg
     3181 aatcccttcg gaaattgttt atgcagtcag cagtttttta aaattttact tgttgttatt
     3241 ttgaaaacat tttataatta atttatgaag aaattagggg tttatttaag cttacccgaa
     3301 atgatcctct tttggattat gctcatcgtg atattgtttt tgatgggcgt tattttttat
     3361 tttgttgctc cgccatggaa atggatagga atagcagtag gtgttatatt tcttcttatt
     3421 agtttttttg ttaatttacg taatacaatt aagcattaca acatattagc tgaacgcgag
     3481 caggttagaa cgattatttc tcattttaca gatgccattg tagcttatga tccagatttt
     3541 cggattactg tttttaatgc gtcagctgaa aacctccttg atttaaaagc aagcgatgtt
     3601 ttgaaccaaa aaataacgcc ggagtgggcg aaaaattcaa aaactaaatt attaacacaa
     3661 gtgcttttcc ctagtttagc tccggcaatg agctggaaaa gtgatccggc tgtctttcca
     3721 aatataatgg atttatcttt tactgatcca attttagaat taacagtaac aactattcgc
     3781 gtaacagatt caaaaggaaa aattttggga ttttttaaaa ttataaagga tcgcacgcgt
     3841 gaagaagaaa tcctcaaaac taaatcggaa tttttaactg tagcagctca tcaaatgcga
     3901 acacctcttt ccggtattaa gtggagttta gagacgctat tgggcgaggg caatgaaaat
     3961 ttaacagaga tgcagcgtga acttttgcag aaaaatttag aagctactga taaaatttta
     4021 cgcttagcta acgatctttt agatgtagcc aatattgaat cagggcgatt tggctatgaa
     4081 tttgtgcagg cagatctgat ggatttaatt cagaaaagca ttagtgatta tctttattta
     4141 gccgatcaac ataaaattaa aatttattta gaaccaccac cagatggatt ggagcctttt
     4201 aaatttgatc cgatgcgtat taaaatcgtt attcaaaatt tgttagaaaa cgctctacgt
     4261 tacaatgtag aaaatgggca gattatagtg aaggtagaaa aaaaacctcc ttatgttgag
     4321 gtatcagtaa gcgatacggg cattggcatc cctaaagatg aattgccaaa attgtttact
     4381 aaatttttcc gagctagtaa tgtattgaaa tacgaaacag agggtacggg tttgggattg
     4441 tatattacca aaaacatcat tgaagcgcac ggcggaaaaa tttgggctga atcaattgag
     4501 aaccgaggat caacttttta ttttactttg ccaatggatg agaaattaat tcccaaacgt
     4561 cacatctctt cggtaatata aagttaaaat taaaagaaac cgaagaaaaa agcagcgaag
     4621 ttgttagtga tgattagata aaaccaaaga ggaatctaaa ttttttaaaa tttgttttac
     4681 attttaaata aaaggctaaa attgatttag cccttgactt tttgataaaa atttgattta
     4741 atagtatcag tgggataagt tgaaattttg atcaacttgt ctcagtttta aaaatttaaa
     4801 aggaggttta gagatgaaat tctggctttt ttctttgatt ggcgtgattt ttttattatt
     4861 gttttttatc cgtccttggg gaccggaaaa agagtggcta acaattgtta cagcattagg
     4921 tttttattgt tggttaggct ggctgtttgt tcaagggctg aaagaggaca aaaataaaaa
     4981 gagaaataag caatcttggt gaatccagtc ggtttcaacc cgacggacca catcaagatt
     5041 gagattatag gaggtttaga gatgaaaatt ttatcagttt gggagcgtat atctgatgcg
     5101 ctccaaaggg aatgggttga caagtctcga aaggggcttg tcgaaggcgt gcaggtggat
     5161 cgggaaagga aacagctcaa agcccaatgt attatttcgt cgcagaacga ggtggttcca
     5221 gtcgtacgga tggataagcg gcctgactgg tttagaattt ccattagggt gccgagctgg
     5281 aggaaaattg tggcagttac ggaagataga attttgtatc ccattgaaaa agagggatac
     5341 aagttactct tctttcggaa cgccacagta ctcgagttct gtcgcggcct tctttccgcc
     5401 acttactatg ctgtaagagc agaaggcagc agattctttt taattgaatc ttctcgcttc
     5461 gagctgggag tccctcgttt tattgaaacg agggaaggaa ttcgtgattt tctggttgaa
     5521 aatcatatcc cgcaagacct gcttgaaccg gcgctcgagt tgatccagaa gcagaagaag
     5581 gatttttcca atcctgtttg gaaagaagga ctggaaaaat ctaaggttaa gccttcacaa
     5641 aaagtggagg taccgaagga gattcgacca gcaatgaaag ttccgaggca accggaagaa
     5701 aaggccccaa tagaagttcc tatgaaaact tccgatgagc cagcaaagct ccgtcgctct
     5761 cctaaaaagc gggagcggcg ggaacgagat gaaaagcctc gccggctacg attccagatg
     5821 gacggtgatg acgtagaatt tcggggagtc agaggactct ccttaaaaga tttagaaaag
     5881 taaaaaataa gcggctttgt tcgaagaaca aagccgcttt ttattttata ttaataaaaa
     5941 gcgggtgtag tggcggcgaa aaatttcaga gaaatttttc gccgccagtc gtcttggatg
     6001 cgtaatacaa ttcagcgctt tgtacaacta aataaaaaaa tttgaaattt ttaaatttta
     6061 tgataaaata ataaaatata aacttataac acctatgaaa aatttattaa aaaatattgc
     6121 tattggaatt ttgatttttt taattttgag taccattttt tcttatttct ggannnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttaaag atgtagctga tttagacgaa
     6301 gctaaacagg aattaatgga agtagtagag tttcttaaaa atccaaagaa gtttttagat
     6361 gttggcgcaa aaattccgcg cggtgtttta ttaatggggc cgccggggtc tggaaaaaca
     6421 ttattagctc gagcagtagc aggcgaggcg ggcgtgccat tttttcatat ttcaggcgca
     6481 gaatttgtag agctttttgt tggagttggt gcgagtcgag ttcgcgatgc tttcactact
     6541 gccaagcgag ccgcgccttc cattttattt attgatgaaa ttgatgctgt tggacgagaa
     6601 agaggtgcgg gtttaggcgg aggtcatgat gagcgcgaac aaacactcaa tcagatttta
     6661 gtagaaatgg acggatttga tagagaaacg aatgtgattg tgatggcagc gactaaccgc
     6721 ccagatattt tggatccagc gcttttaaga ccaggcaggt ttgatcgccg aattgtttta
     6781 gaccttccgg atttaattgg acgagaagaa attctaaaaa ttcattgtcg aggcaaaatg
     6841 ttagagaatg gcgttgattt aaggcgagtg gcagagcgaa cacctggttt ttccggagca
     6901 gacttagcaa atttagttaa cgaagcggca attttagcag cgcgtcgcaa taaaaaagaa
     6961 attgggcagt ctgaactttt agagtcaata gaaaaagtaa ttttgggacc agaaagaaag
     7021 agccgttata tttccgataa agagaaacac attattgctt atcatgaagc tggtcatgct
     7081 ttggttactt actactcacc cgacgctgat cctgttcaga aagtttccat tattgctcgt
     7141 gggcaagccg gtggttatac attaaaaatg cccttagagg atcgtcgttt acaaactaga
     7201 aagcaatttt tagcagagtt ggcgattctt ttaggtggtt ataccgcaga aaaactttat
     7261 ttaggcgata tctctactgg cgctgcaaat gatttgaaag aagcatcaga gcttgctcgg
     7321 aaattagtaa tgcaatttgg aatgtctgag aaattaggac cagttacttt tggcaaaaca
     7381 gaagaaatga tctttttggg gcgagaaatc tcaacagtaa gaaattattc agaggaaaca
     7441 gcagaaaaaa ttgataaaga agtagc
//
LOCUS       DAOSMC010000019         7132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_11643_length_7132_cov_19.961707, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000019 DAOSMC010000000
VERSION     DAOSMC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11643_length_7132_cov_19.961707"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..589
                     /locus_tag="PL075_02205"
     CDS             80..589
                     /locus_tag="PL075_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOD97027.1"
                     /translation="MIVVDTNMLMRSFNFVEQLGKIEWVRVVVPRAVYIEIRERANLD
                     LLKKLKNQPQNCNLKQQEKTIILAMKTWPKLREKIQKREWLIIKVKNAGLITDLRREA
                     RIARLESISNTDLKVLAVAVILRDEGSKVKLLTLDRELAILARRMGIRVPRWHGARKY
                     FWREEILNF"
     gene            798..1145
                     /locus_tag="PL075_02210"
     CDS             798..1145
                     /locus_tag="PL075_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97028.1"
                     /translation="MVFFTSCAPFGTTIAVDTNLPWRTSGLTVINTTPYTFSILIDGV
                     ENAKVGPYGKFAAGLWNGSYYGFEVAVSVVGVEQVFAHTEKVWVWPSSYSRYSSVLTI
                     RQDEDGQIWVERR"
     gene            1322..2887
                     /gene="infB"
                     /locus_tag="PL075_02215"
     CDS             1322..2887
                     /gene="infB"
                     /locus_tag="PL075_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOD97029.1"
                     /translation="MLFKSNAFKEWTKRNFSDKIEKQNMMVKQSEKQLNAVSRPPIVV
                     VMGHIDHGKTTLLDTIRKTNVAARESGGITQHIGAYEILVESKNPEYNQRKITFIDTP
                     GHEAFSAIRARGTKVCDIAILVIAADEGVKPQTVEALNLIQQAKIPFIVALNKIDKPE
                     ANPDRVKTQLAELGVLVEGWGGNVPVINISAKEGKNIQELEELILLVADMEGIVAYKD
                     GPVNGVIVETKFENRRGVEVTAIIKNGVLKIGDPIYTAFAIGKVRTIEDCNGKPLKEA
                     TFSSPVLITGFKEMPQVGEEFFVGEKTEEEIKKADIKRDIKSKTSDQEKNENMLNLIL
                     KADTIGSLEAMKKILSNLVLPEGATLKIISEDIGDVFVSDIALAETAPAIILGFRVKI
                     PKDLQIIIQSKNIRIYTFDIIYEMEKFLKQEAENLFKPQEPPIKGRVEILALFSKKRS
                     EQLIGGRVMEGRIVKGAQVKVQRGEEIIGQGKILNLQSSKIDVKEAQEGKECGILIDS
                     STEIAVGDQLIFQ"
     gene            2920..>3026
                     /locus_tag="PL075_02220"
     CDS             2920..>3026
                     /locus_tag="PL075_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOD97030.1"
                     /translation="MNLKEFDYYLPKEFIAQKPISPRDHSRLMVVDRKTG"
     assembly_gap    3027..3126
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3127..3206
                     /locus_tag="PL075_02225"
     CDS             <3127..3206
                     /locus_tag="PL075_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOD97031.1"
                     /translation="IFKAYEEAIKNKYRFFSFGDAMFII"
     gene            3230..4999
                     /gene="polX"
                     /locus_tag="PL075_02230"
     CDS             3230..4999
                     /gene="polX"
                     /locus_tag="PL075_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOD97032.1"
                     /translation="MNNWEIAKILSDISFYLEMEGEPFKPRAYEKAASTIEALDVPLQ
                     EIYQKQGIKGIEEIPGVGVSIAEKIEELLKTGRLKYYDQFKKKYPFDLEILRRVEGLG
                     PKSLKKLYEILKIKNLADLEKAAHEGKIRKIEGFGEKTEQNILKSIEFLKQGGNRFVL
                     GFIYPQVESIVARLSKLKEVQKIVAAGSVRRMKETIGDLDILIVSENPMSVMDYFVNL
                     PDVFQVVAHGETKSAIRLKNGLDIDLRVVPKESFGAALNYFTGSKDHGIHLREIAIKK
                     GYKLNEYGLFKIPKSKNKNQKEIKIAGRTEEEIYNALGLDYIEPEMREDWGEIEAAAN
                     HQLPKLISYNDIQGDLQIQTNWSDGANSIEEYVAEAIKQNLKYIAITDHTKSLAMTGG
                     CDEKKLLKQMQTIDNINHKLEKDGLNFKILKSAEVNILKDGSLDIEADVLAKLDLVAA
                     AVHSHFNQNKREMTERICKAIRNPLVDIIFHPTGRVILRRPSYKIDVEKIINEAKKNG
                     TCLEIDAYPDRLDLKDEHIKKAIEAGVNLSIDSDAHSITHLNYLKFGIAQARRGWAAK
                     KNIINTRLWPEMFKLTKASKRKK"
     gene            4951..5496
                     /locus_tag="PL075_02235"
     CDS             4951..5496
                     /locus_tag="PL075_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOD97033.1"
                     /translation="MARNVQINKSKQKKKIKFEVSVGIFLFRREKGKILWLLLHYPAG
                     HWDFVKGHIEKNEKIEETIRRELAEETGIRNLKLIPRFQKTLQYWFNNKRYSGRDEMV
                     FKRVIFLLGETEEKEIIISEEHLEGRWFTTKEALSIITFKNVKKYFLQAVAFLKKSGI
                     YNYYNYNAKKDFKKNKRIGDN"
     gene            5450..6940
                     /locus_tag="PL075_02240"
     CDS             5450..6940
                     /locus_tag="PL075_02240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOD97034.1"
                     /translation="MPKKISKKTKESGIIKKNQQKKSSVNSYKTISNKSSKNNKDKIK
                     LHFYGAGKEVEGSCFLVEYKNEKILIDCGIRRDVRSQLNYQQLPFKAEEIKAVILTHA
                     HIDHSGLMPLIYKKGFRGKIYATPPTIDFCTLLWDDAYNLMENAFEEQHSPLLYTRED
                     VDEALNLFSPQPYYQEIGISQNISFVFYNAGHILGSAIVLLKIKKNNGQETKILFSGD
                     LGNSFNLLLPDTDIISEEADYCLVESTYGDRIHEDAEIRKDLLEDVVEEIIANRGVLM
                     IPVLAVERTQEILVELNELIVNHRVPLVPIFMDSPLAIKVTEVYQKYLNYIKIEAFKF
                     VSNGRGLFNFPGLTFTLTTEESKAINAVPPPKVILAGSGSSEGGRILHHEKRYLPDPH
                     SVLLIVSYQAKNSLGRQLLEGVSEVEIHNETIKVNCRVEQITGYSAHADQKMLLNWIK
                     ELRFRLKKLFVVHGEEAAANTLAQKVRDNFGIITEVPNNGDWVELD"
     gene            7009..7095
                     /locus_tag="PL075_02245"
     tRNA            7009..7095
                     /locus_tag="PL075_02245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7043..7045,aa:Ser,seq:cga)
BASE COUNT         2460 a         1002 c         1503 g         2067 t
ORIGIN      
        1 agatataatt taggtagaaa attaaataag gaggtaaata acaaagcttt ttaaaaatta
       61 aataaggagg tgatacatga tgatcgtggt ggataccaat atgttgatgc gtagttttaa
      121 ttttgtagaa cagttaggga aaatagagtg ggtgcgagtg gttgtgccca gagcagttta
      181 tattgagatt cgcgaacgag cgaatcttga tttgttgaag aagttgaaga atcaaccgca
      241 gaattgtaat ttaaaacagc aagagaaaac gattattttg gccatgaaaa cttggccgaa
      301 gttgcgggaa aagattcaaa aaagagaatg gttgattatt aaagttaaaa atgcaggatt
      361 gataacagat ctacgaagag aagctcgaat agcaaggcta gaatccattt ccaataccga
      421 tttgaaagtt ttagcagtgg ctgttatttt aagagatgaa gggtcaaaag taaaactttt
      481 gactcttgat agagaattgg ctattttggc gcggcggatg ggtatccgag tgccaagatg
      541 gcatggtgcc agaaaatatt tctggagaga ggagatttta aatttttaaa taaaaggagg
      601 tctaaatgaa agaaaaattt aggattttaa attttttgga agaccaagat ccattttggg
      661 tctgggcctt ctggattgaa gtagggttat tttttctaat aatagccctg ctactaactt
      721 gagaggaggt taaaatgaga aaaattcagt ggaatgatgc cttttggtgg cttcttttgt
      781 tgcttttctt gttcttattg gtgttcttta caagttgtgc tccttttggt acgacgattg
      841 cggttgatac caatttgccg tggagaacga gtggactgac agttataaat actaccccgt
      901 acactttttc tattttaata gatggtgtag aaaatgccaa ggttgggccg tatgggaagt
      961 ttgccgctgg tttgtggaac ggaagctact atggttttga agttgctgta tcagttgtag
     1021 gagtagagca ggttttcgct catacggaaa aagtatgggt atggccatca tcctattcgc
     1081 ggtactcatc tgttctgacc attcgtcagg atgaggatgg tcagatctgg gtggagcgta
     1141 gatagtggag cgtagataaa aaaatttaaa agggattcta aaaaagaatc cctttttgtt
     1201 aacggcagct gttatgctat agcataacag cttagtaatc attctaaaac aattcctaaa
     1261 acgatgccta aaacgatgcc gctggtgtta tgctatagca taacaccagc ggcatcagta
     1321 tgtgcttttt aaatccaatg catttaaaga gtggacgaaa agaaattttt ctgataagat
     1381 agagaaacaa aatatgatgg ttaagcaatc agaaaaacaa ttgaatgccg tttcgcggcc
     1441 acctattgta gtggtgatgg gtcatattga tcacggaaaa accactcttt tagatacaat
     1501 tcgtaaaact aatgtagctg ctcgagaatc aggtgggatt actcagcata ttggcgctta
     1561 cgaaatttta gtagaaagca aaaatccgga atataatcaa cgaaaaatta cttttattga
     1621 tacacccggt cacgaagctt tttcggccat tagagctcgc ggcactaaag tatgcgatat
     1681 tgctattttg gtgattgcgg ccgatgaggg agtaaaacct caaacggttg aggctttgaa
     1741 tttaattcag caagccaaga ttccttttat tgtggcgctc aataaaattg ataaacccga
     1801 agcgaatccc gatcgagtaa aaacacagtt agcagaattg ggcgttttgg tggaaggatg
     1861 gggaggcaat gtgccagtga ttaatatttc ggctaaagaa ggaaagaata ttcaagaact
     1921 cgaagagtta attttactgg tggctgatat ggaaggcatt gtcgcttata aagatggacc
     1981 agtcaacggc gtgattgtgg aaacaaaatt tgaaaatcgg cgaggcgtcg aggtgacagc
     2041 aattattaaa aatggcgtat taaaaattgg cgatcccatt tatactgctt ttgctattgg
     2101 taaagtgcgg acaatagaag attgtaatgg caagccattg aaagaagcca ctttttcttc
     2161 ccctgtttta atcactggtt ttaaagaaat gcctcaagtg ggagaagagt tttttgttgg
     2221 tgaaaaaact gaagaagaaa ttaagaaggc ggatatcaaa cgggatatta aatccaaaac
     2281 atcagatcaa gaaaaaaatg aaaatatgct caatcttatt ttgaaggccg acaccatcgg
     2341 ttcactagaa gcaatgaaaa aaattttaag caatctagtt ttgcccgaag gagcgactct
     2401 gaaaattatt agtgaggata ttggagatgt ttttgtttct gacattgcac ttgcagaaac
     2461 agcgccggct ataattttag gttttcgagt aaaaattccg aaagatcttc agatcatcat
     2521 tcaatctaaa aatattcgca tttatacatt tgatattatt tatgaaatgg agaagttctt
     2581 aaaacaagaa gcggagaatc ttttcaagcc gcaagaacca cctattaaag gtagagtaga
     2641 aattcttgct ttgtttagca aaaaacgcag tgagcagctt atcggcggtc gagtgatgga
     2701 gggaagaatt gtgaaaggag ctcaggtaaa agttcaacga ggtgaagaaa ttattggtca
     2761 aggaaaaatt ttaaatttac agtcaagcaa gatagatgtt aaagaagcgc aggaaggaaa
     2821 ggaatgcggc atattaattg atagcagcac ggaaattgct gtgggagatc aattaatttt
     2881 tcaataaata ttatgtattg ccggatttga ttttgaaaaa tgaacttaaa agaatttgat
     2941 tattatttgc ctaaagaatt tattgctcaa aaaccaattt ctcctcgtga tcattcccgt
     3001 ttgatggttg ttgatagaaa aactggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnntaat ttttaaagct tacgaagaag cgattaaaaa taaatatcgt ttttttagtt
     3181 ttggtgatgc gatgtttatt atttaaaaga aggagattat aataagagaa tgaacaattg
     3241 ggaaattgct aaaattttat ctgacatctc tttttattta gaaatggagg gcgagccatt
     3301 taaaccccgc gcttacgaaa aagcagcatc tacaattgaa gcattagatg tgccactgca
     3361 agaaatttat cagaagcaag gcatcaaggg aattgaagaa atcccaggag ttggagtttc
     3421 tattgcagaa aaaattgaag aattgttgaa aacagggcgg ctcaaatatt atgatcaatt
     3481 taaaaagaaa tatccttttg atctggagat cttaaggcgc gtagaaggcc taggcccaaa
     3541 atctttaaaa aaactttatg aaatattaaa aatcaaaaat ttggctgatt tagaaaaagc
     3601 cgctcatgaa ggaaagatta gaaaaattga gggatttggc gaaaaaaccg agcaaaatat
     3661 tttaaaaagt attgagtttt taaaacaagg aggcaatcgt tttgttctgg gctttattta
     3721 tccgcaggtg gaatcaattg ttgctcgact ttcaaaatta aaagaagtgc aaaaaattgt
     3781 ggcagctggt tcagtaagga ggatgaaaga aacaattggc gaccttgata ttttaattgt
     3841 ttctgagaat ccaatgtcag tgatggatta ttttgttaat ttaccagatg tttttcaagt
     3901 tgtagctcat ggcgaaacaa aatccgctat ccgcttaaaa aacgggttgg atattgattt
     3961 aagagtagta cctaaagaat catttggcgc agcgctaaac tattttactg gctcaaaaga
     4021 tcacggcatt catttacggg aaattgccat taaaaaaggt tataaattga atgaatacgg
     4081 attatttaaa attccgaaat ccaaaaacaa aaatcaaaaa gaaataaaaa tagcaggaag
     4141 gacagaagaa gagatttata acgccttagg tttagactac attgaaccgg agatgcgaga
     4201 agattggggc gaaattgaag cagcggctaa tcatcaattg ccaaaattaa ttagctataa
     4261 tgatattcaa ggtgatctcc aaattcagac taactggagc gatggagcga attctattga
     4321 agaatacgta gccgaagcaa taaaacaaaa cttaaaatac attgctatta cagatcatac
     4381 caaaagcttg gcaatgactg gcggttgcga tgaaaaaaaa ttattaaaac aaatgcagac
     4441 cattgataat ataaatcata aattagaaaa agatggcctt aactttaaaa ttttaaaatc
     4501 agccgaagta aatattttaa aagatggtag tttagatatt gaagcggatg ttttagctaa
     4561 gctagatttg gtggccgcag cagttcattc gcattttaat caaaataaaa gagaaatgac
     4621 tgaaagaatc tgcaaagcta ttcgtaatcc tttggttgat ataatatttc atcctacggg
     4681 tcgagttatt ttgagacgac catcttataa aattgatgtg gaaaagatta ttaacgaagc
     4741 taaaaagaat gggacttgtt tggaaattga tgcgtatcca gatcggctag atctaaaaga
     4801 tgaacatatc aaaaaagcca tcgaagcggg cgtaaatctt tccattgatt ctgatgccca
     4861 ttcgataacc catttaaatt atttaaaatt tggcattgct caggcgcgac gcggttgggc
     4921 tgctaaaaaa aatattatta atacacgttt atggccagaa atgttcaaat taacaaaagc
     4981 aagcaaaaga aaaaaataaa atttgaagta tcagtgggca tttttctctt tcgaagagaa
     5041 aaagggaaaa tattgtggct actgcttcat tatcctgccg gtcactggga ttttgttaaa
     5101 ggacatattg aaaaaaatga aaaaatagag gaaactataa ggagagagct tgcggaagaa
     5161 acaggaatca gaaatttgaa acttattcca cgttttcaaa aaacattgca atactggttt
     5221 aataacaagc gatattcagg aagagatgaa atggttttta aaagggtcat ttttttatta
     5281 ggcgaaactg aagaaaaaga aattataatt tctgaagaac atttagaagg cagatggttt
     5341 acgactaaag aagctcttag tatcataact tttaaaaacg ttaaaaaata ttttcttcaa
     5401 gccgtcgcgt ttcttaaaaa gtcaggaatt tataattact ataattacaa tgccaaaaaa
     5461 gatttcaaaa aaaacaaaag aatcggggat aattaaaaag aatcaacaga aaaaatcttc
     5521 agtaaatagt tataaaacta tttcgaataa aagcagcaag aataataagg acaaaataaa
     5581 actccatttt tatggcgctg gaaaagaagt tgaaggtagt tgttttcttg tagagtataa
     5641 aaatgaaaaa atcttaattg attgcggcat tcgtcgagat gttcgcagtc agcttaatta
     5701 tcagcagttg ccttttaaag cagaagaaat taaggcagtt attttgactc atgcccatat
     5761 tgatcatagt ggtttaatgc cgctgattta caaaaagggt tttcgaggta agatttatgc
     5821 tacgccgcca accattgatt tctgtacatt attatgggat gacgcctata atttaatgga
     5881 aaacgctttt gaagaacaac attccccgct cctttatacg cgagaagatg tggacgaagc
     5941 actaaatctt tttagtcctc aaccttatta tcaagaaata ggcatttctc agaatatctc
     6001 ttttgttttt tataacgccg gtcacattct tggatcagca atcgttcttt taaaaattaa
     6061 gaaaaataat ggtcaggaaa caaaaattct tttttctggc gatttgggca attcttttaa
     6121 tttgctattg cctgacactg acattattag cgaagaagcc gattattgtt tagttgaatc
     6181 aacttatggc gatcgcattc atgaagatgc agaaataaga aaggatttat tagaggatgt
     6241 tgttgaagag attatagcca atcgaggcgt tttgatgatt cccgttttgg ccgtagaacg
     6301 aacgcaggaa attttagtag agttaaacga attaatagtg aatcatcgag tgcctttagt
     6361 tccaattttt atggatagtc ctttggcaat taaagtgacg gaggtttatc aaaaatatct
     6421 taattatatt aaaattgaag cttttaaatt tgttagtaat ggaaggggat tattcaattt
     6481 tcccggttta acctttaccc taacaaccga agaatcaaaa gccattaatg cagtgccgcc
     6541 accgaaggtt attttggctg gatctggatc atcagaagga ggtcgcattt tacatcacga
     6601 aaaaagatat ctgcctgatc cacacagcgt tcttttaatt gtttcttatc aagctaaaaa
     6661 ttcgttagga cgacaattat tagagggcgt ttctgaggtt gaaattcata atgaaactat
     6721 taaagtcaat tgtagggtag agcagattac tggttattct gcgcatgccg atcagaagat
     6781 gctccttaat tggattaaag aactaaggtt tcgtttaaaa aaactatttg tggtacacgg
     6841 agaagaagca gctgctaata ctttggctca gaaagtgcga gataattttg gaattattac
     6901 tgaagtgcca aataatggtg attgggtaga attggattaa agtgttaagc taaagttaaa
     6961 aagtagctag ataattcaaa gattttaaaa ggaaagagct atattttcgg aggcgtgcca
     7021 gagtggttga atgggccgca ctcgaaatgc ggtatcccgg tttcgggatc ggaggttcga
     7081 atcctcccgc ctccgctttt ataaaatatt cagagcaagc caactgcttg gc
//
LOCUS       DAOSMC010000020         6681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_12614_length_6681_cov_20.742529, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000020 DAOSMC010000000
VERSION     DAOSMC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12614_length_6681_cov_20.742529"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..537
                     /locus_tag="PL075_02250"
     CDS             274..537
                     /locus_tag="PL075_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97035.1"
                     /translation="MPDIIPQTKPDNNPYNQSDWDFILQRDLIKESGLDKFEWIQLYS
                     PKFRQLVEEKSEIIKTLRDDPEKLKEQFKKWLYEPENHNENIH"
     gene            551..874
                     /locus_tag="PL075_02255"
     CDS             551..874
                     /locus_tag="PL075_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97036.1"
                     /translation="MAQKTIFQKIYFVIIIILVVLTLIIVIYTFVNKNKESALEEGFD
                     ESLIACQMSGGQIATSSCCLSATDYPNTCLIGACGCAPENSHEIKICDCGVDKCFNGN
                     ECVNR"
     gene            917..989
                     /locus_tag="PL075_02260"
     tRNA            917..989
                     /locus_tag="PL075_02260"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:950..952,aa:His,seq:gtg)
     gene            1133..1513
                     /locus_tag="PL075_02265"
     CDS             1133..1513
                     /locus_tag="PL075_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97037.1"
                     /translation="MKRKIIWFLVLMGCFGMIFFGVKKLKNPEIPSAEVKSAVLAAIS
                     QKIDLKKLDPDMRLIWIAKDEKGEKIQPVLVRIGITDGTYTEIKEVIFGEIKEGEFVV
                     TGRATADQINTRTPDINQVFRSLR"
     gene            1533..2009
                     /locus_tag="PL075_02270"
     CDS             1533..2009
                     /locus_tag="PL075_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97038.1"
                     /translation="MVYFYNLTNGLEVKITDLNQHPHLVRIQSSHVESKAWDRLFQQL
                     SDELLFYLAIGEKCIIIDGSPNPNHSHISRIAVPVIQYVLTRLWLKEKLAPDMIDDEY
                     LERIYRSLTQSTKRKLKYYRKFLITNEVKLNCYVFPNCKDGKYDWFKEKIKERIRK"
     gene            2105..2470
                     /locus_tag="PL075_02275"
     CDS             2105..2470
                     /locus_tag="PL075_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97039.1"
                     /translation="MKKLALKFSRFFKGYFSLSVLLEDAKQKKVEKIFIQPRIYIEEF
                     PGSTLYVITGHVEVFASELPKTYIDLSFQQIVKKGVEEAEKLAYRECEQDLQERTEKI
                     KEIIEKAGLQTAIGKIKTQ"
     gene            2648..4171
                     /gene="rny"
                     /locus_tag="PL075_02280"
     CDS             2648..4171
                     /gene="rny"
                     /locus_tag="PL075_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOD97040.1"
                     /translation="METLILISLITLFLGIIIGYFVRQVVVRNQVKSAEQKIKAQLEE
                     AKTKAKEIVLDAKGKAATILAEAQKEEKERKKELSAFEQRLNRREELLNQKLTETEAK
                     NHNLEENIKRVKEIKEKVEELRLETKKELERVARLSTEEAKKELFTQLEKTHQQEIAD
                     LLKRLEKEKRDEIEKESFKILETTVQRLSRSVSSEITTSVFTIDNEELKGKIIGREGR
                     NIRALERLTGVDIIIDEAPDAITLSSFDPLRRELARISLEKLIKDGRIQPAKIEEKVE
                     EAQKELNEKIKEAGENAAFELGILDLPKEIIYLLGRLNYRTSYGQNVLQHSIEAAHLA
                     KMIAAELGANVEVAKKGALLHDIGKAIDHEVEGTHVELGRKILKKYNIPEEIIKAIEP
                     HHEDYPFETPESYIVAAAEAISASRPGARRDSLENYLKRLENLEKIALSFDGVQKAYA
                     VQAGRELRIFVIPEKIDDYGALNLAKQVATKIHDELNYPGEIKVNVIRETRAVEYAR"
     gene            4261..4536
                     /locus_tag="PL075_02285"
     CDS             4261..4536
                     /locus_tag="PL075_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOD97041.1"
                     /translation="MTKEGLAETILSKFELASKKQAKEIVETIFETITKTLARGEEVA
                     ISGFGTFRAARRAAREGINPRTGEKIQIPASIKPKFRASKALKEAIK"
     gene            complement(4620..5189)
                     /locus_tag="PL075_02290"
     CDS             complement(4620..5189)
                     /locus_tag="PL075_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOD97042.1"
                     /translation="MDNSNKKDNKKKIRKLVSQAQHGNAESFGEIYELFVERIYRYIY
                     LKTNNKEEAEDLTQQVFTKAWEALPQFKFYKNPFSSWLYSIARNLIIDFYRKKKPDFS
                     LDSENALDIVDDLDLAEQFAIQEEVRRVVEFINQLPSEQKELLLLRFVDDLSYDEIAA
                     IMNKSPLTLRVIQHRALKRLKGIMEKDGD"
     gene            5270..5404
                     /gene="rpmH"
                     /locus_tag="PL075_02295"
     CDS             5270..5404
                     /gene="rpmH"
                     /locus_tag="PL075_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOD97043.1"
                     /translation="MSTTYQPKKKKRAKTHGFLKRMKKPGGRNIIKRRRQKGRSRLAV
                     "
     gene            5405..5755
                     /gene="rnpA"
                     /locus_tag="PL075_02300"
     CDS             5405..5755
                     /gene="rnpA"
                     /locus_tag="PL075_02300"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOD97044.1"
                     /translation="MKYRSLKKEKDFQRVFQKGKKINCPELTLIFLPRQNDPINYVAI
                     ITSKANFRKATQRNLIKRRLKAILRQINLAKNGLDIIIIAKTPLLTKKFEDLKIVVES
                     IFQKAKLNDIEFKQ"
     gene            5770..6513
                     /locus_tag="PL075_02305"
     CDS             5770..6513
                     /locus_tag="PL075_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOD97045.1"
                     /translation="MGNLFHIILVQPILNLLILIYNILPWPDIGLATIILTVIIRLIL
                     APFFHKSTKQQIMINRLQPAIQKIQKEYKDDKEKLAKAQLELYKKNKVNPFGNLAITL
                     IQLPIMLAVYRVFLLGFGPEIIQNNLYSFISQPQTINTLFLGFINLNKANIFMAILAA
                     VVQYLSTKLTMSSLKQSKRHQKDQSPNQKMANALQKQMIFLGPVMTFIILVGLPSILG
                     LYWIATTLFSIAQQWYVLKHFHFEEIELE"
BASE COUNT         2438 a         1133 c         1143 g         1967 t
ORIGIN      
        1 gagagcggaa gctcgagcga cttgtagccg agtcccaccc cgggcacaaa cgaatgaaag
       61 cagaaagtcc aaaatcaaaa ttcaaaaatg cagcaaagat gcgatagaaa ttttttagaa
      121 aatttccatt gcatcaatca ttgtaattca ctcgcgctat tatttattat ggactacttg
      181 atcaatattt agaattttca tcgcaattca cctcaattta tatttttata ttgacactgg
      241 cttttctcta tttctatggt aaaataaagt tgtatgccag acataattcc tcaaaccaag
      301 cctgataata atccctataa tcaatctgac tgggacttta ttcttcaaag agatttaatt
      361 aaagaatcag gattagacaa attcgaatgg atccaacttt attcccctaa attcaggcaa
      421 ttagtagaag aaaaatcaga aattattaaa accttacgag atgatcccga aaaacttaaa
      481 gaacaattta aaaaatggct ttatgaacca gaaaatcaca atgaaaacat tcattaagaa
      541 ttaaaaaaat atggctcaaa aaactatttt ccaaaaaatc tatttcgtca ttattattat
      601 ccttgttgtt ttgactttaa ttattgtcat ttataccttt gtaaataaaa ataaagaatc
      661 ggccctagaa gaaggttttg atgaatcact aatcgcctgt caaatgagcg gtggtcaaat
      721 tgccacttct tcttgttgtt taagcgctac agattatccc aatacctgcc ttattggcgc
      781 ttgcggttgc gctccagaaa atagtcatga aataaaaatt tgtgattgcg gagttgataa
      841 gtgcttcaat ggtaatgaat gtgtcaatcg ataattttga caaaattcga gttgcgaata
      901 aaatactaat atcatggtgg ccttagttca gtggctagaa cgcctcgttg tggccgagga
      961 ggtcaagggt tcaaatccct taggccaccc taataaaatt caaattatat aaattcaaaa
     1021 gatcaaaaat ctcgattcaa cctcaatggc tagagttgtt gtttttttat ttaaaagcgc
     1081 taaaatagaa ttggcaactt aaaaacttta tcgaaattaa aaggagggaa ctgtgaaaag
     1141 aaaaattatt tggtttttgg ttttaatggg atgttttggg atgatttttt tcggagtgaa
     1201 aaaattaaaa aacccagaga ttccgagcgc tgaggtcaaa agcgctgttc ttgcggctat
     1261 ttcccaaaaa attgatttaa aaaaattaga tccggatatg agactgatct ggattgcaaa
     1321 agatgaaaaa ggcgaaaaaa ttcagccagt tttagtaaga attgggatta ccgatggaac
     1381 gtacacggaa atcaaagagg tcatttttgg agaaatcaaa gaaggagaat ttgttgtcac
     1441 tggtcgagca acagcagatc aaattaacac tcgaacacct gatattaatc aagtgtttcg
     1501 gtcattaaga tagaaaagaa aggaggtgtg aaatggtata tttttataat ttaactaatg
     1561 gattggaagt aaagataacg gatttaaatc agcatcctca cctagtacga attcaatcat
     1621 cacacgtgga aagcaaagcc tgggatcgtc tttttcaaca attaagcgat gaattgcttt
     1681 tttacctggc tattggtgaa aaatgtatta ttattgacgg ttcgcccaat ccaaatcatt
     1741 cccacatttc gcgaatagca gtacctgtta tccaatacgt tttaacccgt ctttggctca
     1801 aggaaaaact cgctccagat atgattgatg acgaatacct tgaaagaatc tatcggtctt
     1861 taactcaaag taccaaaaga aaattaaagt attacagaaa attcttaatt accaatgaag
     1921 taaagctgaa ttgctatgtc ttccctaact gcaaagacgg gaaatatgat tggtttaaag
     1981 aaaaaataaa agaaagaata agaaaataat taaccttaaa gggtggtctt tagaccaccc
     2041 ttttcttgac aaaaaattca aaaaagttaa tctataatca gcaacttaat ttcaaggagg
     2101 aaaaatgaaa aagttggctt taaagttttc gagatttttt aagggttatt tttctcttag
     2161 tgttcttctt gaagacgcta aacagaaaaa ggtggagaag atattcattc agccccgcat
     2221 ctatattgaa gaatttcccg gctcaaccct ttatgttatc actggtcatg tagaagtttt
     2281 tgcttctgaa ttgccaaaaa cttacatcga cctgtcgttc cagcaaatag ttaaaaaggg
     2341 ggtcgaagaa gcagaaaaac tggcctatcg agaatgtgaa caagatctgc aagaaagaac
     2401 agaaaagata aaagagataa tcgaaaaggc aggattacaa acagcaatag gaaaaatcaa
     2461 aactcaataa aaatatctta aagacgagca ctagctcgtc tttttttctt gcaatttttc
     2521 aaaaatagga ttataataaa aatgaattta atttgtattt agtcagttaa cttttaattt
     2581 cgctctaaaa aaattgtgaa acaaaaattt ttccaatcgc attttttgaa aggcgaaatc
     2641 caatcatatg gagacgctta ttttgatttc tctaatcact ttgtttttag gtattattat
     2701 cggatacttc gttcgtcaag ttgttgttag aaatcaagtc aaatccgctg agcaaaaaat
     2761 aaaagctcaa ctcgaagaag caaaaactaa agccaaagaa attgttctag acgctaaagg
     2821 caaggcggcg actattttgg ctgaagcgca aaaggaagaa aaagaaagaa agaaagaact
     2881 ttcggctttt gaacaacgct taaatcggcg cgaggaatta cttaatcaaa aacttaccga
     2941 aaccgaagca aaaaatcata atttagaaga aaatataaag cgcgtcaaag aaataaaaga
     3001 aaaagttgaa gaactgcgtt tagaaactaa aaaagagctc gaacgagtag cgcgcctttc
     3061 aacagaggaa gcaaagaaag aactctttac tcaattagaa aagacccacc aacaagaaat
     3121 agccgatctt ttaaaaagac tggaaaaaga aaaaagagat gaaatagaaa aagaatcttt
     3181 taaaatatta gaaacaactg ttcagcgctt atcgcgatct gtaagcagtg aaattaccac
     3241 ctctgttttt actattgata atgaggaatt aaaggggaag attattggtc gagaaggtag
     3301 aaatattcgc gctttagaac gccttacagg cgttgatatt attattgacg aagcgcccga
     3361 cgccattact ctttcctctt ttgatccttt gcgtcgagaa ttagctagaa tatctttaga
     3421 aaaattaatc aaagacggcc gtattcaacc agctaaaatt gaagaaaagg tggaagaggc
     3481 tcaaaaagaa ctgaatgaaa aaataaaaga agcaggtgaa aatgctgctt ttgaattagg
     3541 tattttggat ttacccaaag aaattattta tcttcttgga agattaaact atcgaacaag
     3601 ctatggccag aatgtgcttc agcattctat tgaagcagcc cacttggcca aaatgattgc
     3661 cgccgaattg ggtgctaatg tagaagtggc taaaaaaggc gctttattac acgacattgg
     3721 aaaagctatt gatcatgagg tagaaggtac tcatgtggaa ttaggccgca aaattcttaa
     3781 aaaatataac attcccgaag aaattattaa agcgattgaa cctcaccatg aggattatcc
     3841 ttttgaaacg cctgaatcat atattgtagc agccgctgaa gccatttcgg ctagtcgtcc
     3901 aggcgcccgt cgcgatagct tagaaaacta cctgaaacgc ttggaaaatc tagaaaaaat
     3961 cgctctttct tttgacggcg ttcaaaaagc ctatgctgtt caagcgggtc gggaattgcg
     4021 tatttttgta attccagaaa aaattgacga ttatggcgct ttgaatttgg caaagcaagt
     4081 tgctactaaa attcatgatg aattaaatta tccaggagaa attaaggtta atgttatccg
     4141 agaaacaaga gcggttgaat atgcgcgcta attatccaca cccctatttg gaaagctcat
     4201 aaaatgtgct ataatctaat aaagctgagt tattcaaagg tcgattaatt atcaaaaaaa
     4261 atgacaaagg aaggattagc ggagacaatt ttgagcaaat tcgaattagc cagtaaaaaa
     4321 caagctaaag aaattgtaga aaccattttt gaaaccatca ccaaaacttt agccagaggc
     4381 gaagaggtcg ctattagcgg atttggtact tttcgcgcgg ctcgtcgagc agctcgtgaa
     4441 ggcattaatc ctcgaaccgg cgaaaaaata caaattccag cttccatcaa gcccaaattc
     4501 agagccagca aggctttaaa agaagctata aaataaagat tttaaagact tttcaaaaat
     4561 tgtccggacg aattcaaaaa ccccttatta ataaggggtt tttgaataac atcaaaaaac
     4621 taatcgccat ccttctccat tattcccttt aatcgtttta atgctcgatg ctgtattacc
     4681 cgtaaagtta aagggctttt attcataatt gcggctattt catcataaga taaatcgtca
     4741 acaaaacgca aaagcagcaa ttctttttgt tctgaaggta attgattaat aaactctaca
     4801 actcgacgaa cctcctcctg gatagcaaat tgttcggcca aatctaaatc atcaactata
     4861 tctaaagcgt tttcactatc taagctaaaa tctggctttt ttttgcgata aaaatctata
     4921 attaaatttc gggcaataga ataaagccaa ctactaaaag gattcttata aaacttaaat
     4981 tggggcagcg cttcccaagc ttttgtaaaa acttgctggg ttaaatcttc ggcttcttct
     5041 ttattattgg ttttcaaata aatataacga taaatgcgtt caacaaaaag ctcataaatt
     5101 tcgccgaaag attcagcgtt tccatgttgt gcttgagaaa ctaattttct tatctttttt
     5161 ttattatctt ttttattact attatccata gaaaccaagc gttttcttta tcattataca
     5221 tcatacataa ttattgcgaa aatcaaaatt ctatgttaga atggctgata tgtcgactac
     5281 ttatcaaccc aaaaaaaaga aaagagcgaa aacgcacggc ttcctgaaac gaatgaaaaa
     5341 accaggagga agaaatatca taaaaaggcg ccgccaaaag ggtcgttctc gtttagcagt
     5401 atagatgaaa tatcgctccc ttaaaaaaga gaaagatttt caacgagttt ttcaaaaagg
     5461 gaaaaaaatc aattgtccag agctaactct tattttttta ccacgacaga atgatccgat
     5521 caattacgta gccattatta ccagcaaggc caattttcgt aaagccactc aaagaaacct
     5581 tattaaaaga aggctgaaag caatccttcg gcaaattaat cttgcaaaaa atggattaga
     5641 catcatcatt attgccaaaa cacctctttt aactaaaaaa tttgaggatc taaaaattgt
     5701 cgttgaatca atctttcaaa aagcaaaatt aaatgatatc gaatttaagc agtaatcatt
     5761 tgttcattta tgggtaacct ttttcacata atccttgtcc agccaatttt aaatttatta
     5821 attttgatct acaacatttt accttggcca gatattggat tagccactat tattctaacg
     5881 gtgattatcc gtcttatttt ggcgcctttt tttcataaaa gcaccaaaca acaaataatg
     5941 atcaatcgac tgcaaccggc tattcaaaaa attcaaaaag aatacaaaga cgataaagaa
     6001 aaattagcca aagctcagtt agaattgtat aaaaaaaaca aagtcaatcc ttttggcaat
     6061 ttagccataa ccctaattca attacctatt atgttagcag tttatcgtgt ctttttatta
     6121 ggtttcgggc cagaaataat ccaaaacaat ctttactctt ttatcagtca gccccaaaca
     6181 attaacactc tatttctggg ttttatcaat ttgaacaaag caaatatttt tatggctatt
     6241 ttagcagcgg ttgtccaata tctatctacc aaactcacaa tgtcttctct aaaacaatca
     6301 aaacgacatc aaaaagatca aagccctaac caaaaaatgg ctaatgcttt acaaaaacag
     6361 atgatctttt taggaccggt gatgactttt atcattcttg ttggtttacc ttcaatttta
     6421 ggcttatatt ggattgcaac tactcttttt agtatcgctc agcaatggta tgttttaaaa
     6481 cattttcatt ttgaagaaat tgagttagaa taatatttat ttacaatata aatatatgga
     6541 agaaattact caaatagtta aaaacattct atcatttctt gatttagaag acgcaagtgt
     6601 tgatgttgtt gaagcggatg ataatttatc catcaatatc agaaccgaaa atggtcattt
     6661 ccttattgga ccagaaggat c
//
LOCUS       DAOSMC010000021         6640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_12712_length_6640_cov_21.190737, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000021 DAOSMC010000000
VERSION     DAOSMC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12712_length_6640_cov_21.190737"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..102)
                     /locus_tag="PL075_02310"
     tRNA            complement(31..102)
                     /locus_tag="PL075_02310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Arg,seq:tct)
     gene            303..374
                     /locus_tag="PL075_02315"
     tRNA            303..374
                     /locus_tag="PL075_02315"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:336..338,aa:Glu,seq:ttc)
     gene            379..450
                     /locus_tag="PL075_02320"
     tRNA            379..450
                     /locus_tag="PL075_02320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:412..414,aa:Pro,seq:tgg)
     gene            566..1057
                     /locus_tag="PL075_02325"
     CDS             566..1057
                     /locus_tag="PL075_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97046.1"
                     /translation="MESFEEMLAIACGFLKCFAEKFPHKKVGSLILQKMMFLFEQEKK
                     WDLGFSLNHRGPFSELVESILIRAQSAGNIGIYPYQRSGHYIQLLKCPQEVSDEDKKL
                     IEQLVEKYGKLCSDDLVILTTALFLKDRFEVESKSQLIDLISESRPQMERAEIKKIIN
                     LIE"
     gene            1140..1880
                     /locus_tag="PL075_02330"
     CDS             1140..1880
                     /locus_tag="PL075_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOD97047.1"
                     /translation="MMHQREYNLYQPNANDSFSLSRSKIELFIECPRCFYLDVRLGIA
                     RPPGFPFTLNQAVDSLLKKEFDIHRVAEAAYPLLQRYGMKLRPLNDERIKDWRNNRIG
                     IRYYHQPTNLEIYGAIDDVWVDENGMIYIVDFKSTSSQNEPTLDDAWKDSYKRQMEIY
                     QWLFRQNGFNVSNTGYFVYCNGKTDRAAFDGKLEFDISVLPYEGQDSWIEPTIFEMKK
                     TLDSDKIPLASKNCDYCQYVALRYQVEK"
     regulatory      1929..2039
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2083..3045
                     /locus_tag="PL075_02335"
     CDS             2083..3045
                     /locus_tag="PL075_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOD97048.1"
                     /translation="MNQKQLIKTAEFVSPKHPDKICDQMADALLDAYLKKDPFVHTAI
                     EILAGHQLVVVMGEIKSFFELGEEAIKKIFQKLVGKNFELKIKLVKQSPEIFRGVEKG
                     GAGDQGIMNGYATSETKSLMPLEYELARNLCRKIFQQYPYDGKTQVTVEGKKVISVVA
                     SFQNVPKNKLEKLVRSIIKAEKYYINPAGDWKIGGFEADSGLTGRKLVIDAYGPNVPV
                     GGGSFSGKDATKVDRSGAYMARKIAVDYLKKYKAQEVFVKLAYAIGEEKPLMKEVIVD
                     GQEDEIKGYDLTPRGIIKFLNLRKPIFEKIARWGHFGRNFLWDN"
     gene            3081..4700
                     /locus_tag="PL075_02340"
     CDS             3081..4700
                     /locus_tag="PL075_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97049.1"
                     /translation="MKTQKRFVLILLFCSFVIGNLFLNFSHILAAGNETVNLYFFWGN
                     GCPHCAKEEIFLQQLTQKYPEVKIYSYEVWYNAQNQDLLIQVGQTLKTDIQGVPFTVI
                     GPHAISGYYDEKTTGVQIENYVKECLSQECADPVALLVGLAPIVSITPSVTPQITPQS
                     SPTPTLSPLPTIKAYFFESSGCPLCKAEKKYLESLTDQYPNLQLEYLELSQKENLEIF
                     KKTLDKFQLENAAVPFLVIGENYILGWKDESTTGRAIQDAINCAIETGCPDVVGNLMS
                     SINLETSNSILPEVVEVPLIGEVKIKNLSLPILAIILGGIDGFNPCAMWVLVFLISLL
                     LGMKDRKRMWILGFVFLFTSAIVYLLFMAAWLKLILFVGAIFWVRLIIALIAVIGGAY
                     NIKKFIQEKNKINITCEVVDEKQKRKTIERMKAAVSKQSLFWAILGIIILAASVNLVE
                     LVCSAGLPVVFTQVLAMSNLSSGQYVLYMLLYIMMYMLDDVIIFVAAMMALKITAKSS
                     QYTRYSHLIGGILMIIIGLLLVFKPAWLMFG"
     gene            4779..5204
                     /locus_tag="PL075_02345"
     CDS             4779..5204
                     /locus_tag="PL075_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOD97050.1"
                     /translation="MKLIPRNPFEDLEKWFEDENWALPIVPSSAFKEPAMDIYQTDKD
                     VVAELNIPGFDPQKIDISIDNQVLTVKGETEETEEEKKRDYWRKEIRKGSFERMVRLP
                     AEVDENNVEAVYKNGVLKIVMPKKTSSSQTKKIPIKTKD"
     gene            5204..6280
                     /locus_tag="PL075_02350"
     CDS             5204..6280
                     /locus_tag="PL075_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97051.1"
                     /translation="MSKTLPIVFLLITLVVVLFVLRYSPVPVPLPGADSNKQNTFQTT
                     PQPTAYTGPRPETVIVNGPKDWEEVSTTPYVVFQYIALWEGDFRDILFETKVDEIDKD
                     WQRSSGNSRAIQLLPGEHTYHFQVRATTKDGIYDYTPAMRIFRGNISSQASRVKVNSV
                     VPYATPQKIIILNSGPDIDLTNWTIETSAGFFTITTGVRLFRPDSQTIHQNIILKTGD
                     SLVVVEGSSPLSFNFYLNRCFGYLTNEYNFNSLFVKNCPRPSYTDISYFSSACQQFIN
                     NLDTCQIPSSNDINRFSNDPACQQFIKDYYHYSSCVDRYQSAPDFFKKEWYVFVNRDQ
                     FISTTHDRIVLRDDEGLVVDVYQY"
     gene            6329..6400
                     /locus_tag="PL075_02355"
     tRNA            6329..6400
                     /locus_tag="PL075_02355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6362..6364,aa:Arg,seq:cct)
BASE COUNT         2133 a         1045 c         1327 g         2135 t
ORIGIN      
        1 ttttaggatg acgattttga atttttatcg tgcccccggg aggaatcgaa cccccatccg
       61 ctccttagaa gggagctgtt ctatccgttg aactacgggg actattttaa aaatgctaca
      121 cttattctaa tagaaaaaaa atttaaaaca agttgaaaaa aataaaaaaa ttagcataat
      181 aaaaaaagcg gctatcgttt aataattaag acaacgctct ttcctcgcag aatatatttg
      241 ttcgttttct ctcgcttggt attctgtaaa catcccgctg ctcattaaaa tttttataat
      301 ctgccgccat cgtctaatgg ctaggacaac actctttcaa ggtgtaaatc cgggttcgat
      361 tcccggtggc ggcgcttacg ggctgtagtg taatggctag cacgagtcct ttgggaggat
      421 ttagtccggg ttcaaatccc ggcagcccga ctaaaataaa aattactcat ccaggtgttt
      481 tttatttgac aaagaaataa tgtcatttaa gatcagagat agtttttaag aacaaaattt
      541 aataatttaa taaaaggagg tttgaatgga atcgtttgaa gaaatgctgg ctattgcttg
      601 tggtttcttg aagtgttttg cggaaaaatt tccccataaa aaggttggtt cattgatttt
      661 gcagaaaatg atgtttcttt ttgaacagga gaaaaaatgg gacctcggtt tttctcttaa
      721 tcatcgagga ccattttccg agttggttga gtcaatactc attcgggccc aatcagcggg
      781 aaatatcgga atttatcctt atcagcgaag cgggcattat attcaattat taaaatgccc
      841 tcaagaagtt tctgatgaag ataaaaaatt gattgaacag ctggtagaaa aatacgggaa
      901 attatgttct gatgatctgg taattttgac aacggctctg tttttgaaag accgatttga
      961 agtggaaagt aaaagtcagt taattgattt gatttccgaa tctcggccgc agatggaacg
     1021 cgctgaaatt aaaaaaataa ttaatttaat tgaatgaaaa atttctgcag gcgacgcgaa
     1081 gcgtcgcctt tttattttca aaaatttgga aaaattaatt aatctgttaa aataataaaa
     1141 tgatgcatca aagagaatat aatctttatc agccaaatgc gaatgattca ttttctttga
     1201 gtcgttccaa aatagaactt tttattgaat gtccgcgctg tttttattta gatgttcgat
     1261 taggcattgc tcgaccgccg ggttttcctt ttactctgaa tcaagcagtt gattctttgt
     1321 taaaaaaaga atttgatatt catcgtgttg ctgaagctgc ttatccgtta ctgcaacgtt
     1381 atgggatgaa gttgcgaccg cttaacgacg aacgcatcaa agattggcgt aataatcgca
     1441 ttggaattcg ttattatcat cagccaacca atttagaaat ttacggcgcc attgatgatg
     1501 tttgggtaga tgagaatgga atgatttata tcgttgattt taaaagtaca agctcgcaaa
     1561 acgaaccaac gcttgatgac gcttggaaag attcttacaa aaggcagatg gaaatttatc
     1621 aatggctctt tcgtcaaaac ggatttaacg tgtccaatac cggctatttt gtttattgca
     1681 atggtaaaac cgatcgcgcg gcttttgacg ggaaattaga atttgatatc tcagttttac
     1741 cttatgaggg tcaagattct tggattgagc caactatttt tgaaatgaaa aaaactttag
     1801 attcagacaa aattccttta gctagtaaga attgcgatta ttgtcagtat gttgctttaa
     1861 gatatcaagt agaaaaataa atttgacagc aatagtatat tggatataat gaaataaaat
     1921 caaataatga gttattaaga gtgcggggag ggacctggcc cgataatccg ccgacaaccc
     1981 tttaagaatt tttcttaaag aaggtgccaa atccagaccc ataaagggga agataacaag
     2041 tcggaactct ttcttttcag gaagagtttt ttaatttaaa atatgaatca aaaacaatta
     2101 attaaaacag cagagtttgt ttcacccaaa catccggata aaatttgcga tcaaatggcc
     2161 gatgctttgc tcgacgccta cttaaaaaaa gacccatttg ttcatacagc tattgaaatt
     2221 ttagctggtc atcaattggt agtggtgatg ggagaaataa aaagtttctt tgaactgggc
     2281 gaagaggcaa taaaaaaaat ttttcaaaaa ttggtgggca aaaattttga attaaagatt
     2341 aagttagtaa agcaaagtcc agaaattttt cgcggcgttg aaaagggcgg cgccggagat
     2401 cagggtatca tgaatgggta tgctacgtcg gaaacaaaat ctctgatgcc tttggaatat
     2461 gaattggctc gaaatttatg tcggaaaatt tttcagcaat atccttatga tggaaaaact
     2521 caagtgaccg ttgaaggaaa aaaagttatt agtgtggtgg cgagttttca aaatgtgcca
     2581 aaaaataaat tagaaaaatt ggtgcgttca attattaaag cagaaaaata ttatattaat
     2641 cccgctggcg attggaagat tggcggattt gaagctgata gtggtttgac cggtcggaaa
     2701 ttagtaattg atgcttatgg ccccaatgtg ccagtgggcg gtggttcttt tagtggcaaa
     2761 gatgcgacca aagtggatcg cagcggcgct tatatggcac gtaaaatagc cgtagattat
     2821 ttaaaaaaat ataaagcgca agaagttttt gttaaattgg cttacgcgat cggagaggaa
     2881 aaaccattaa tgaaagaagt aattgttgat ggtcaggaag atgaaattaa gggttatgat
     2941 ttaactccaa gaggtattat aaaattttta aatttaagaa aacctatttt tgaaaaaatt
     3001 gctcgttggg gacactttgg tcgaaatttt ctttgggata attgattaaa aaattaaaat
     3061 acaataaaat agaaaacatc atgaaaacgc agaaacgttt tgtcctcatt ttattatttt
     3121 gcagttttgt tatagggaat ctatttttaa atttttctca tattttggct gccggcaatg
     3181 aaacagttaa tctttatttt ttctggggca atggctgccc gcattgtgcc aaagaggaaa
     3241 tttttttgca acaattaacg caaaaatatc ctgaagtaaa aatttattcc tatgaagttt
     3301 ggtataatgc gcaaaatcag gatttactta ttcaagtagg tcaaacatta aaaacagaca
     3361 ttcagggcgt gccatttact gttattggtc cacatgcaat ttcgggttac tatgatgaaa
     3421 aaacaactgg tgtgcaaatt gaaaattatg tgaaagaatg tctaagtcaa gagtgtgctg
     3481 atccagtagc tttacttgtt ggtttagcgc caattgtttc aataactcct agtgttacac
     3541 ctcaaattac tccgcaatca tcaccgacgc caacattatc accccttcct acgatcaagg
     3601 cctatttttt tgaaagtagc ggatgccctt tgtgtaaggc agaaaaaaaa tatttagaga
     3661 gcctaacgga tcaatatcca aatttgcaac tggaatatct agaactttct caaaaagaaa
     3721 atctcgaaat ttttaaaaaa acattggata aatttcaatt agaaaatgcg gctgttcctt
     3781 ttttggttat tggagaaaat tatattttag gatggaaaga cgaatcaaca acgggtaggg
     3841 ccattcaaga cgctataaat tgcgctattg aaacgggctg tccagacgtt gtaggcaatc
     3901 ttatgtcgtc tattaatttg gagacgagta attctatttt gcctgaggtt gtggaagtgc
     3961 ctttaatcgg tgaagtaaaa attaaaaatt tgtctttgcc aattttagct attattttag
     4021 gtggaataga cggttttaat ccatgcgcaa tgtgggtttt agttttttta attagtttac
     4081 ttttaggaat gaaagacagg aaacggatgt ggattttggg atttgttttt ctgtttactt
     4141 ctgcaattgt ttatttgctc tttatggccg cttggttaaa actaattctt tttgtaggcg
     4201 ctattttttg ggtgaggctg attattgctt tgattgcagt aataggtggc gcttataata
     4261 ttaaaaaatt cattcaagaa aaaaacaaaa taaatattac atgcgaagtg gttgatgaaa
     4321 aacaaaaaag aaagacgatt gaacggatga aagcagcggt gagtaaacaa tccttgtttt
     4381 gggctatcct gggcattatt attttggctg cttcggttaa tctggtagag cttgtttgtt
     4441 cagctggatt gcctgtggtt tttactcaag ttttagccat gagtaattta tcaagcgggc
     4501 aatacgtact ttatatgtta ctttatatta tgatgtatat gcttgatgat gtgattattt
     4561 ttgttgcagc aatgatggcg ctcaaaataa cagctaaatc cagtcagtat acgcgatatt
     4621 ctcatttaat tggtggtatt ttgatgatta ttattggact gctgcttgtt tttaaacccg
     4681 catggttgat gttcggttga cttgattttt agataacatt ttggtataat atctatataa
     4741 attatcctaa aggtcggttt aaataaaatg ttaccactat gaaactgatt cctcgtaatc
     4801 catttgaaga tttagaaaag tggtttgaag acgaaaattg ggcattgccc attgttccct
     4861 cttcggcatt taaagagccg gcaatggata tttatcaaac agacaaggat gtggtcgctg
     4921 aattaaatat tcctggtttt gatccacaaa aaatagacat ttctattgat aatcaagttc
     4981 taactgttaa gggcgaaaca gaagaaactg aagaagaaaa gaaaagagat tattggagaa
     5041 aagagattcg aaaaggctct tttgaacgaa tggttcgttt gccagcagag gtagatgaaa
     5101 acaatgtgga agccgtctat aaaaatggtg ttctgaaaat tgtaatgcct aagaaaacgt
     5161 cttcaagcca aaccaaaaag attccgatta aaaccaaaga ctaatgtcca aaaccttacc
     5221 tatcgttttt cttcttatta cattagtggt tgtgctattt gttttaagat atagccccgt
     5281 gcctgttcct ttgcccgggg ctgattctaa taagcaaaat acttttcaaa caacgcctca
     5341 gccaacagct tataccggac cgcgaccgga aacagtcatt gtcaatggac caaaagattg
     5401 ggaagaggta tctactacac cctacgttgt ttttcaatat attgctttgt gggaaggaga
     5461 ttttcgagac attctttttg aaaccaaagt agatgaaatt gataaagatt ggcagcggag
     5521 ttccggcaat agccgagcaa ttcaactttt accaggagaa catacttatc attttcaagt
     5581 tcgggcaaca acaaaagacg gcatttatga ttatacgccg gcaatgcgaa ttttcagagg
     5641 taatatttct tcgcaggcat ccagagttaa agttaattcg gttgttcctt acgccacgcc
     5701 tcaaaaaata atcatattaa attcaggacc agacattgat ctaactaatt ggacaatcga
     5761 aacatcagca ggctttttta cgattaccac cggagtgcgc ttgttccggc cggatagtca
     5821 aaccattcat caaaatatta ttttaaaaac cggtgattca ttagttgttg ttgagggatc
     5881 aagtcctttg agttttaatt tttatttgaa tcggtgcttt ggttatttaa ccaacgagta
     5941 taattttaat tcgctttttg ttaaaaactg tccgcgtccc agctacaccg acatctcata
     6001 tttttcttca gcctgtcagc aattcattaa taatttagat acctgtcaga ttccctcttc
     6061 aaatgatatc aatcgtttta gcaacgatcc tgcctgccag caatttatca aagattatta
     6121 tcattattct tcctgcgttg atcgctatca atctgcgcca gattttttta aaaaagagtg
     6181 gtatgttttt gtgaatcgag atcaatttat cagtacgact catgaccgaa tagttttgcg
     6241 cgatgatgag ggattggttg ttgatgttta tcaatattag ataaaacatt agataaaaaa
     6301 tagaataaaa ttttttgaaa caattattgc ccctgtagct caaaggatag agcaatcccg
     6361 tcctaaggga gagatgtggg ttcgattcct accaggggca caagggagat taaaatttaa
     6421 aatctgacgc tgggtgttat ttttaatctg ttcggcggaa agcagagagc gtaagcgact
     6481 aaattatctg agtttatttc agatacttta atggattaat gtacctcaaa taaattgata
     6541 gattcttcgg ctttcagcct tagaatgacg attttatttt tgaattctcc cttgtactca
     6601 aattaaaccc cttcaaattt tctttattat cttaacaaat
//
LOCUS       DAOSMC010000022         6356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_13391_length_6356_cov_18.313284, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000022 DAOSMC010000000
VERSION     DAOSMC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_6356_cov_18.313284"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PL075_02360"
     CDS             complement(<1..198)
                     /locus_tag="PL075_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOD97052.1"
                     /translation="MKAQKKLGQNFLINKNALKKIVDALSISENDIIIEIGAGTGNLT
                     QEILKKTSKVIAIEKDKSLVKL"
     gene            complement(209..2200)
                     /locus_tag="PL075_02365"
     CDS             complement(209..2200)
                     /locus_tag="PL075_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOD97053.1"
                     /translation="MIDKFKALEGLNEKQREAVLYTEGPVLIVAGAGSGKTRTLTHRV
                     AYLLATGIKSEEILAITFTNKAAEEMRDRIKQILGENNFFQKLFIGTFHKLGVNILRD
                     LFSLVQRRKNFVIFDEEDSLKLIRECLMELNLPREQFIPVNVRNEISRAKNELILAKD
                     YEQLAENFFQKNVAKVFNLYEEKLIKNNAFDFDDLITLPVHIFLTHPDILEKYQNKWR
                     YILVDEYQDTNTSQYQLIKLLASKYRNLCVVGDEDQSIYSWRQADFRNFLNFEKDWPD
                     AKIIILETSYRLTKNILAAAQEVIKNNKLRREKTLISPNQEGRPVFVIRALDDIEEAE
                     FIAATIQSLVQNYQCQYKDFAVLYRMNVQSRVLEEVFLKFHIPYQIFAGVKFYQRKEI
                     KDIIAYLRILANPADLISLERIINVPSRGIGQKKVEVIFQGADYKKVWENLKKFSETK
                     EFLEIYQQLKNDLNKKSVASLSKELLEKIDYQSYLKQTSSQAEEKWQNAMELIRLAED
                     FDEYEKGDLNQFLEKISLFQEGDEKINDRNYVSLMTLHSAKGLEFNIVFIVGVEEGIL
                     PHVQSQYSDDEMEEERRLIYVGMTRAKEEVYLIWSSRRRMFGNLQINTSSRFLDEIPS
                     DLKQEIELDAFKEESDEKKKKIDPDNPEIELFDDGDYEF"
     gene            2299..3555
                     /locus_tag="PL075_02370"
     CDS             2299..3555
                     /locus_tag="PL075_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOD97054.1"
                     /translation="MFKFAQGLDFKSALAIIQTNPSEIDFISAGEQFIKPICTFFANL
                     YHQFRSISKKIAELGKKKSHPNGFFQSFTSLIERFFWIKQPSFLLVAFLLILTTSILV
                     FNFINKDGASSLKESDRGVIGGPFLPQNSLALLNDAAATISHQNGSLTNNNSISDSEN
                     EIYYLIDHSSIQNPNPTLTSIQTDKNGLLFYKVQKGDTLLSIASDFGISLNTIIWANN
                     LKSEKITPGEELIILPVSGILHEIKTGDTIETIAKMYSASPDKIIAFNNITNSNLKPG
                     EKIIVPDGILSSSQAKTKTATSNLPSYPYYYVLPTTGFNWGTLHAVNAVDIANRCGTP
                     VMASAEGLVILVDWDNGYGNHIKIQHPNNTETLYAHLNQIRVKEGDYVMQGELIGVMG
                     NTGYVIGNPGCHLHFEIRGAKNPFVK"
     gene            complement(3636..>4207)
                     /locus_tag="PL075_02375"
     CDS             complement(3636..>4207)
                     /locus_tag="PL075_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97055.1"
                     /translation="QPLEDGEITISRVQGSLTFPARFILVAAMNPCPCGYYGDPEKEC
                     HCSPSEILKYQKKISGPLMDRIDMQIEVPRVKYDDLKSEQIGESSATIRSRIEEVRRI
                     QKERFKNLARAIYTNSEMSSKEAEKFCLLEKEAEALLKKAVESFYLSARSYYKFLKVA
                     RTIADLNHSDLIRVEDVAEALRYRLKNEN"
     assembly_gap    4208..4307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4308..4930)
                     /locus_tag="PL075_02380"
     CDS             complement(<4308..4930)
                     /locus_tag="PL075_02380"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOD97056.1"
                     /translation="MTAKIYSAAVIGIKAQPIEVEVDLGPGLHSFNIVGLADKAVSEA
                     RDRVSSAIKNSGAAAPQRQNRKITVNLAPADLKKEGSAYDLAIAVGYLLASNQMKAFD
                     ASNKIFLGELALDGSLRPVAGVLPVILMAKEKFKEIILPTKNIREAEIIKGIKIVGCE
                     TLKDVIEYLEGKKEIGFVSNKDLEISQNKKHAVDFADIKGQQQAKRA"
     gene            complement(4953..5345)
                     /locus_tag="PL075_02385"
     CDS             complement(4953..5345)
                     /locus_tag="PL075_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOD97057.1"
                     /translation="MTILKKKKMDKKQLKEFQELLEKEKKELEEKISDLNLKEKELAI
                     KMPDFSASEDSSIEADEIEELNNLLSLRFVWENDLASINEALERIKKGTYGICEECGG
                     LIEIARLKIEPAAKICAKCLKKKNSASV"
     gene            complement(5357..5704)
                     /gene="rplT"
                     /locus_tag="PL075_02390"
     CDS             complement(5357..5704)
                     /gene="rplT"
                     /locus_tag="PL075_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOD97058.1"
                     /translation="MTRVKRGIVGHKKREKVLKEAKGYKWGRKSKERLAKEALLHAQS
                     RSFVGRKQKKRNFRQLWQTKIKAAVLKEGLRYNEFIHLLKINNIAINRKILSELAEKQ
                     PEVFRSIVAEVKK"
     gene            complement(5768..5962)
                     /locus_tag="PL075_02395"
     CDS             complement(5768..5962)
                     /locus_tag="PL075_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97059.1"
                     /translation="MLKTTKSFQKRIKLSKSRKALHRPIKQNHFKAKLSGRSNQDKRK
                     VKPFYKTDEKAIKKYLPYTK"
     gene            complement(5964..>6356)
                     /gene="infC"
                     /locus_tag="PL075_02400"
     CDS             complement(5964..>6356)
                     /gene="infC"
                     /locus_tag="PL075_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOD97060.1"
                     /translation="LAQSKNLDLVEIVNTANPPVVKICNYGKFLYQQEKTEKKQKAKE
                     RTDEIKHIRLTFNIGKHDMEVRARQTEEFLKQGLKVQIELVLRGREKAHKDLATNKLK
                     DFLNSIQHPYKIIQDIKNSPRGLIVTIG"
BASE COUNT         1876 a         1202 c          875 g         2303 t
ORIGIN      
        1 taacttaact agagatttat ctttttcaat cgcaataact tttgatgttt tttttaaaat
       61 ttcctgcgtc aaattgcccg tgccagctcc gatttcaata ataatatcat tctccgaaat
      121 acttaaagca tcaacaattt tttttaaagc atttttatta attaaaaagt tttgacctaa
      181 tttcttttgc gctttcataa ttgaaaaatt aaaattcgta atcgccatca tcaaataatt
      241 ctatttcagg attatcagga tcaatttttt tctttttctc atcactttct tccttaaaag
      301 catctaattc aatttcctgc ttcaaatcag acggaatttc atctaaaaat cgcgaggaag
      361 tgtttatttg taaattacca aacattcgac gtcgcgatga ccaaattaaa taaacctctt
      421 ctttggcgcg cgtcatacct acataaatca acctcctttc ttcttccatt tcatcatcag
      481 aatattgcga ttgaacgtga ggtaaaatac cctcttctac gccaacaata aatactatat
      541 taaattctaa ccctttggcg ctgtgcaatg tcattaaaga aacatagttg cgatcattta
      601 ttttttcatc cccctcttga aaaagtgaga ttttttcaag aaattgattt aaatcgcctt
      661 tttcatactc atcaaaatct tcagctaaac gaatcaattc cattgcattc tgccactttt
      721 cttcggcttg acttgaggtt tgctttaaat aagactggta atctatcttt tctaataatt
      781 ctttagagag agaggccact gattttttat ttagatcatt tttaagctgt tggtatattt
      841 ctaaaaattc tttagtctca gaaaattttt ttaaattttc ccatactttt ttataatcag
      901 cgccttgaaa aatcacctct acttttttct gcccaatgcc tcgcgatggt acattgataa
      961 ttctttctaa actaatcaaa tcagcaggat ttgctaaaat tctcaaatag gcaataatat
     1021 ctttaatttc ttttctctga taaaatttaa cgccggcaaa aatttgataa gggatgtgga
     1081 actttaaaaa cacctcttcc aaaactcgcg attggacatt catccggtaa agcaccgcaa
     1141 agtctttata ttgacattga taattttgaa caagactctg aattgttgca gcaataaatt
     1201 ctgcttcttc aatatcatct aatgctctta tcacaaacac tggcctacct tcttgattgg
     1261 gtgaaatcag agttttttca cgacgtaatt tattattttt aataacttct tgagctgctg
     1321 ctaaaatgtt ttttgttaaa cgataacttg tttcaagaat aataattttg gcatcaggcc
     1381 aatctttttc aaaatttaaa aaattgcgaa aatcggcttg ccgccaagaa taaattgact
     1441 gatcctcatc tccgacaaca cataaatttc tatactttga agcaagtagt tttattaatt
     1501 ggtattgcga agtattggta tcttggtatt catcaactaa aatatagcgc catttattct
     1561 ggtatttttc taaaatgtct ggatgagtta agaaaatatg tactggtaaa gtaattaaat
     1621 catcaaaatc aaacgcgttg tttttaatca atttttcttc atatagattg aacactttcg
     1681 ctacgttttt ttgaaaaaaa ttctccgcta gttgttcata atctttagct aaaattaact
     1741 cattcttcgc tcgtgagatt tcattgcgaa cattaaccgg aataaactgt tctctgggta
     1801 aatttaattc cattagacat tctcgaatga gttttaaact atcttcctca tcaaaaatca
     1861 caaaattttt ccggcgttga actaaagaaa acagatccct taaaatatta acgcctaact
     1921 tatgaaaggt gccaataaat aatttctgaa aaaaattatt ttctcctaaa atttgcttaa
     1981 tgcgatctct catttcttct gcagctttgt tagtaaacgt aatggctaat atttcttccg
     2041 atttaatgcc cgttgctaaa agataagcta cgcgatgggt taaagtgcgt gttttacccg
     2101 aaccggcacc agccacaatt aaaactggcc cttcagtgta caaaaccgcc tccctttgtt
     2161 tttcatttaa accttcgagt gctttaaact tgtcaatcat gacgttattt tagcactttt
     2221 actaaccatt ttccagatta attttaaaaa actgcatttt tttatttaat tatcttattc
     2281 cttcgtattt tctattatgt gtttaagttt gcgcaaggac ttgactttaa atctgcttta
     2341 gctataatac aaactaatcc ttcagagata gattttatct ctgctggaga acaatttatt
     2401 aagcctattt gtacattttt tgccaatctt tatcatcagt tccgatcgat aagtaagaaa
     2461 atagcggaat taggaaagaa aaaatcgcat cctaatggat tttttcaatc tttcacttca
     2521 ttaatagaaa ggtttttttg gattaagcaa ccatcatttt tgttggttgc ttttttgtta
     2581 attttaacca cctcaattct tgttttcaat tttattaaca aagatggggc ttcttcgctt
     2641 aaagaatctg accgaggtgt aatcggcggt ccttttcttc cacaaaattc gctcgctctt
     2701 ttaaacgatg ctgctgccac aatcagtcat cagaatggtt ctttaaccaa taataattca
     2761 atctctgatt cagaaaatga aatctattat cttattgatc acagtagcat tcaaaatccc
     2821 aatcctactc tgacaagcat tcaaacggat aaaaatggcc ttttgtttta caaggttcaa
     2881 aaaggagata ctcttttaag cattgcctct gactttggca taagtcttaa tactattatt
     2941 tgggctaata atttaaagag tgaaaaaatc acccccgggg aagaattaat tatcttacct
     3001 gtttccggaa tcttacatga aataaaaacc ggagatacta tagaaactat tgcgaaaatg
     3061 tattcggctt ctcctgataa aattatcgct ttcaataata ttactaatag taatcttaaa
     3121 cccggagaaa aaattatagt acctgatgga attctttctt catcgcaagc aaaaaccaaa
     3181 acagcaactt ccaatttgcc ttcttatcct tattattacg ttcttcctac cactggattt
     3241 aattggggca cacttcatgc cgtcaatgca gttgatatcg ctaatcgatg cggtactcca
     3301 gtaatggcaa gcgccgaagg actagtaata ttagtggatt gggataatgg ttatggaaat
     3361 catattaaaa ttcagcatcc caacaacacc gaaacgcttt acgcccacct taatcaaatt
     3421 agagttaaag agggtgatta cgttatgcaa ggagagctaa ttggcgtgat gggcaacact
     3481 ggttacgtta ttggcaaccc gggctgccat cttcattttg aaattcgcgg cgctaaaaat
     3541 ccttttgtaa aataaatact aattctccat taacaaacac atgataaaaa aatcgagttg
     3601 agctcgattt ttttatcatg tgttttacaa taacatcaat tttcattttt gaggcgatat
     3661 ctcaaagctt ctgccacgtc ttcaactcta attaaatccg aatggttcaa atcggcaata
     3721 gttcgagcaa cttttaaaaa tttgtaatag ctgcgcgccg aaagataaaa actttctacc
     3781 gcctttttta gtaacgcttc tgcttctttt tctaaaaggc aaaatttttc tgcttctttg
     3841 gaagacattt cactgttagt ataaatcgct cgagctaaat tcttaaatcg ttctttttga
     3901 attcgcctaa cttcttcaat acgcgaacga atggtagccg aggattctcc tatttgttct
     3961 gattttaaat catcgtattt aactcgcggc acctcaattt gcatatcaat acgatccatc
     4021 aatggccctg aaattttctt ctgatatttt aaaatttccg agggactaca atggcactct
     4081 ttttctggat caccataata gccacaagga caaggattca ttgcagccac taaaataaat
     4141 cgcgctggaa aagtcaaact tccctgcacg cgactaatgg taatctcacc gtcttctaat
     4201 ggttgtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa gctctttttg
     4321 cttgttgctg tcctttaata tcagcgaaat caactgcatg ttttttgttt tgcgaaattt
     4381 ctaaatcctt attacttaca aaaccaatct ccttttttcc ttctaaatat tcaattacat
     4441 cttttaatgt ttcacaacca acaattttaa ttcctttaat aatttcggct tctctaatat
     4501 ttttagttgg taaaattatt tccttaaatt tttccttggc catcagaata actggcaaaa
     4561 cgcctgccac cggccttaag ctgccatcta atgctaattc acctaaaaaa attttattgc
     4621 tggcatcaaa tgctttcatt tgatttgaag ccaaaagata acctacggca atagctaaat
     4681 cgtaagccga accttccttt ttaagatcgg cgggtgctaa attaacggtg attttcctat
     4741 tttgtctttg gggagcagcg gcgccgctat ttttaatagc tgaagaaact cgatccctcg
     4801 cctcgctcac tgctttatca gccaatccta caatattaaa agaatgaagg cctgggccta
     4861 aatccacctc cacttcaata ggttgagctt taatcccaat aacagctgct gaataaatct
     4921 tagccgtcat aattttggaa aaataaagaa atttaaactg acgctgagtt tttctttttt
     4981 aaacacttag cgcaaatttt ggctgctggt tcaatcttta atcgggctat ttcgattaat
     5041 cctccgcatt cttcacaaat accataagtt ccttttttaa ttctttccaa cgcctcatta
     5101 attgaggcta aatcattttc ccaaacaaac cttagagata aaagattgtt aagctcctca
     5161 atttcgtcag cttcaataga tgaatcttcg gaagcgctaa aatcaggcat tttaattgct
     5221 aattcctttt ctttaagatt taaatctgaa attttttcct ccaattcttt tttttctttt
     5281 tctaacaatt cttgaaattc cttcaattgt tttttatcca tttttttctt ttttaaaatt
     5341 gtcataattt tatactttat tttttgactt cggccactat tgatcgaaat acttctggtt
     5401 gtttttcggc caactcagat aaaattttcc gattaatcgc aatattatta attttgagta
     5461 aatggataaa ttcattatat ctcagccctt ctttaagcac tgcggcttta atttttgttt
     5521 gccacaattg acgaaaattt cgcttttttt gtttccgacc aacaaaggaa cgactctggg
     5581 cgtgaagtag cgcttcttta gccaagcgct cttttgattt ccgaccccat ttatatcctt
     5641 tcgcttcttt taaaaccttt tctctctttt tatggccaac gattcctctt ttaactcttg
     5701 tcataaaata ataaattcag cgcttattta aatattatta aatatttaaa tcccgcgcct
     5761 ttaaatttta tttggtataa ggaaggtatt ttttgatagc tttttcgtct gttttataaa
     5821 acggttttac cttcctttta tcttgattac ttcgacctga aagtttagct ttaaaatgat
     5881 tttgtttaat aggcctgtgt aaggcttttc tacttttgct taattttatt cttttttgaa
     5941 atgattttgt tgtttttaac atattaacca atagtaacaa ttaagcctcg aggtgaattt
     6001 ttgatatctt ggataatttt ataaggatgt tggatagaat tcaaaaaatc tttcaattta
     6061 ttagtggcta aatctttatg ggctttttct cttcctctta aaacaagttc tatttgaact
     6121 tttaagccct gctttaaaaa ttcttctgtt tgacgtgctc gcacttccat atcgtgtttt
     6181 ccaatattaa atgtcaaacg gatatgttta atttcatcag tgcgttcttt agctttttgt
     6241 tttttttctg tcttttcttg ctgatacaaa aatttgccgt aattgcaaat tttaaccacc
     6301 ggcggattag ccgtattaac aatttcaacc aaatccaaat tttttgattg agctaa
//
LOCUS       DAOSMC010000023         5793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_15132_length_5793_cov_21.476124, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000023 DAOSMC010000000
VERSION     DAOSMC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15132_length_5793_cov_21.476124"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..3173)
                     /locus_tag="PL075_02405"
     CDS             complement(177..3173)
                     /locus_tag="PL075_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOD97061.1"
                     /translation="MKKESKQIKQYLQNIVKNIPFENLPTKWQGFDLVTFSKDKTLFD
                     FQQDALKNAIIGLWLYFKDKNANKQDLFKLYQNNEFKENFDYDLKKKAEGKTIKYLLE
                     YDQDYPAVDSKISFAHFINRMSFWMATGSGKTLIIVKLIELLGKLIAEKELPARDILF
                     LAHRDDLLDQFKNHVEEFNSFNFDTKINLKNLRDYESVKRENTLPFAKNEITVFYYRS
                     DLISDEHKEKIVNFKNYDNDGKWYILLDEAHKGDKEDSKRQILYSILSRNGFLFNFSA
                     TFTDPRDFATCVFNFNLSKFVEEGYGKHIYVSAGDISAFRDENDFSPIEKQKIVLKTL
                     LLLTYVNKYFEKIRQVDDSLYHRPLLLTLVNSVNVEDADLELFFRELEKIAKNEIRVD
                     LLDEAKQELVQEFCDNAKFVFEELECVIDADLVSKLDYKDILKYVLNANTPGKIEVLK
                     IPGNRQELIFKLTTSDKPFALIKIGDISGWLKDKLEGYEINESFENESYFRQINRDDS
                     DINILMGSRSFYEGWDSNRPNLLLFVNIGVGSDAKKFVLQSVGRGVRIEPQKYQRKRL
                     QNLYNAGVVKDQLFEDVKDLILPIESLFVFGTNAKNLEEIFATLKAEKQDKSLGKVFI
                     VNPEREGKILLIPVYKTTERIFAEEENPQKYPVSPEDLDITSQFYEFLGDKVALAKYD
                     CEVKVLEKAKESFAEKERYYDFSERNSLYEPELILDRIFDYLGVKSKEFDKFKKLEKE
                     IVHFEKVRFCGEGFNQISAKIQQVKSYPEKEKRLQQLKLEFDKHKNLDKYTTEVQNLE
                     KNYVKEIEYEYNSKKIKIKYLANHYYLPVIVSETEIIDYLNHIINVDSEVKFIEQLED
                     YLAKPDNVFRQFDWWMFSKLDQTLDEVFIPYYNHKESRMDNYHPDFIFWLQKGNDYLI
                     LFVDPHGTAYSDINKKIDYFSKIFEVEGTKENKTFPSDRYGIKEKFNIRVKLLLKPAQ
                     GGLTSIGDPYKRYWFDNFDDFARKIS"
     gene            complement(3179..3943)
                     /locus_tag="PL075_02410"
     CDS             complement(3179..3943)
                     /locus_tag="PL075_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97062.1"
                     /translation="MSTQREKIIKQAREILENSPKGIRYADLIRGISEALPDVKINTI
                     HGTIWEFKQGIDKGEVKDVARPEKGLYILQKYLKEREYGDVEYRESEEIKEVKEEDFY
                     KLFADYLVNELEECTKAVPLGVNRFQDRWGTPDVIGTYRILGIGHIQPPIEVISAEIK
                     TDINQLITSFGQACSYKLFSHKVYLVIPKDAREVDIKRVESLCLKFGIGLIIFDRTNK
                     ERPEFEILTRAIKSEPDYFYLNKYLRLIEDIIEELF"
     gene            complement(3946..>5793)
                     /locus_tag="PL075_02415"
     CDS             complement(3946..>5793)
                     /locus_tag="PL075_02415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD97063.1"
                     /translation="FRVFEKQSEVDFFINKNAKAFLQEQFKLWSYQYFWEGAKEWSAD
                     RVNELQILKDIAFKIIDFISQFEDELVKIWNKPKFVKNSNYVITLDRIKDISLVEKIL
                     KHKNINEQIKEWQELGIVDESFKVIDIFTSDLMSKHLSEKYQHLPIDTKYFKDLELEI
                     LSQFEDLDKSLDGWLIKSENYQALNTILPKFKEKVQTIYIDPPFNLDSSDQFLYRTNY
                     KNANWATLLENRLKIAKDWLNEKGSIFVRCDYNGNWIVRYLMDEIFGKGNFRNEIFVK
                     RGYVPKGLTNQYLTGIDSIFFYAKDLNKIAFKGAKLKISEEDRKWISLDMPGQRKTYE
                     LQVRHFFGKPWLPPKGQHWGLSQQKISEYEKLGWIRINNGRKYKDTQDNEVKGMPEYL
                     KEPELLLDTNWTDIKSYETHNTFFATENSEILLKRVIEGMEYDEQSLIMDFFLGSGTT
                     TAVAHKLGRKWLGVEMGEHFYTVILPRMKKVLAYDKSGISKEVKEYQGGGFFKYYELE
                     QYEEALANCKYEDSDLFNSPSKTPYQEYVFLKDEKMLKALEIDYENNKVKVDLDKLYS
                     NIDIAETLSNLTGKWIKKISDGEVEFEDGLKINTKDLDYKLIKPLIWWE"
BASE COUNT         1690 a         1138 c          798 g         2167 t
ORIGIN      
        1 ggggttcttc cgtaggaact ccgctggaga aatccctccc tctccgccaa ggatactttg
       61 actaatacca aaagattatt tatgatggga agtataaatt ttcctttttt gattgccgcg
      121 ttcagatgag gcgatgattt ttatgccatc ttttatgaaa ttattataat ttcaatttaa
      181 ctgattttgc gcgcaaaatc atcaaagttg tcaaaccagt agcgtttgta aggatctcct
      241 atagacgtta aacctccttg cgctggctta agtaataatt ttactctgat attaaatttt
      301 tctttgatac catatctatc ggagggaaaa gttttatttt cttttgtccc ttctacttca
      361 aatatttttg aaaaataatc tattttttta tttatatctg aataagcagt cccatgtggg
      421 tcaacaaata atattaaata atcgtttcct ttttgtagcc aaaaaataaa gtcgggatga
      481 taattatcca ttctgctttc cttatgatta tagtatggta taaatacttc atccagagtt
      541 tggtcaagtt ttgaaaacat ccaccagtca aattgcctaa atacattatc gggtttagca
      601 agatagtctt ccaattgctc aataaatttt acttcgctat caacattaat aatgtgatta
      661 aggtagtcaa ttatttcggt ttctgaaact attaccggaa gatagtaatg gttagccaga
      721 tacttgattt tgattttttt tgaattgtat tcatattcta tttctttaac ataatttttc
      781 tccaaattct gtacctctgt agtgtattta tcaagatttt tatgcttatc aaattctaat
      841 ttcagttgtt ggagacgttt ttctttctct gggtagcttt tcacctgctg tatttttgcg
      901 gatatttgat taaaaccttc gccacaaaat ctaacttttt caaaatgcac gatttctttt
      961 tctaacttct taaacttatc aaactctttg ctctttactc cgagataatc aaaaatccgg
     1021 tcaagaatta actccggctc atagagcgag tttctttcgc taaaatcgta atatcgctct
     1081 ttttcagcaa aactttcttt tgctttttcc aaaaccttaa cttcgcagtc atacttagcc
     1141 aaagcaactt tatcgcctaa aaattcataa aattgtgatg ttatatctaa atcttcgggg
     1201 ctgacaggat atttttgcgg gttttcttct tcagcaaaaa ttctctcggt ggttttataa
     1261 acaggtatta aaagaatttt cccttctctt tcaggattga caataaatac tttgcctaaa
     1321 ctcttgtctt gtttctctgc tttcagcgtt gcaaatattt cttctaaatt cttcgcattt
     1381 gtcccaaaga caaacaaact ttcaatcggc aagatcaaat ctttcacgtc ctcaaacaac
     1441 tgatctttta caactccggc attatataag ttttgaagcc gtttcctttg gtatttttgt
     1501 ggctctattc ttacgcctct acccaccgat tgcagaacga atttcttggc gtcgcttcca
     1561 accccgatat tcacaaataa aagcaaattt ggtcgattag agtcccaacc ttcataaaaa
     1621 gagcgcgaac ccattaaaat atttatgtcg gaatcatcgc gattgatctg tctgaaataa
     1681 ctttcgtttt caaaactttc gtttatctcg tacccctcaa gtttatcttt cagccaacca
     1741 gaaatatcgc cgattttgat caaagcaaat ggtttatcgg atgttgttaa cttaaaaatt
     1801 aactcctgtc tattaccagg gatttttaaa acttcaattt tgcccggtgt gtttgcgttc
     1861 aaaacatatt ttaaaatatc cttgtaatcc aattttgaaa ccaaatcagc gtctattaca
     1921 cactccaatt cttcaaatac gaacttggca ttatcgcaaa attcctggac aagttcctgt
     1981 tttgcttcat cgagcaaatc taccctaatt tcgttctttg ctattttttc caattctcta
     2041 aaaaacaact ccaaatcagc atcttctaca tttacagaat taaccagggt caaaagaagc
     2101 gggcgatggt ataaggaatc gtcaacctgt ctgatctttt caaaatactt attgacataa
     2161 gtcaaaagca aaagcgtttt caaaacaatt ttctgttttt caatcggcga aaaatcattc
     2221 tcatctcgaa aagcgcttat atctccagcc gaaacataaa tatgtttacc atacccttct
     2281 tctacaaact ttgaaagatt gaagttaaaa acgcaggttg caaaatccct cgggtcggta
     2341 aaggtggcgg agaaattaaa taaaaatccg tttcttgaaa gtatggagta taaaatctgc
     2401 cgcttgctgt cttctttgtc gcctttgtga gcttcatcta aaagaatata ccatttgccg
     2461 tcattatcat aatttttgaa attgacgatt ttttctttgt gttcatctga aatcaaatcg
     2521 gagcggtaat aaaaaacggt aatttcgttt ttggcaaatg gcagggtatt ttcccgcttg
     2581 acgctttcgt aatctctcaa atttttgaga tttatctttg tgtcaaaatt aaaactgtta
     2641 aactcctcaa catgattttt gaattggtcc aaaaggtcgt cgcggtgtgc caaaaataaa
     2701 atatcccttg caggtaattc tttttcggca atcagcttgc ccaaaagctc aatcaattta
     2761 acgataatca gtgtcttgcc cgaaccggtt gccatccaga aactcatccg attgataaaa
     2821 tgggcaaaag aaatttttga atctacggca ggataatctt ggtcatattc taaaagatat
     2881 tttatcgtct taccttcggc ttttttcttt aaatcgtaat caaaattctc tttaaattca
     2941 ttgttttgat aaagtttaaa taaatcttgt ttatttgcat ttttgtcttt gaaataaagc
     3001 cataatccta tgatcgcatt tttcagcgca tcttgttgaa aatcaaaaag tgttttgtct
     3061 tttgaaaacg ttaccaaatc aaacccctgc cactttgtcg gcagattttc aaaagggata
     3121 ttttttacaa tattttgtaa atattgttta atttgttttg attctttttt catcttaatt
     3181 agaataattc ttctattata tcttcaatta atctcaaata cttatttaga taaaaataat
     3241 ctggttcgct tttaatcgcc cttgttaata tttcaaattc tggcctttct ttattggttc
     3301 tatcaaaaat tattaaacca ataccaaatt taagacagag cgattctact cttttgatgt
     3361 caacttcacg cgcgtctttt ggaataacta aataaacttt atggctaaaa agtttataag
     3421 aacaagcttg accaaaggat gtaatcaatt gattgatatc tgtttttatt tcagcgctta
     3481 taacttcaat cggcggctga atatgaccaa tccctaaaat tctgtaagtt ccaataacgt
     3541 ctggcgtacc ccacctatct tgaaatctat taacacctaa tggcactgct tttgtgcatt
     3601 cttctaattc atttactaaa taatcagcaa ataatttata aaaatcttct tccttaactt
     3661 cttttatttc ttcactttcc ctgtattcaa catctccata ttctctttct ttcaaatatt
     3721 tctgtaaaat atataaaccc ttttctggtc tagcaacatc tttaacctct cctttatcaa
     3781 ttccttgctt aaattcccaa atagttccgt gaatggtatt aattttcaca tctggtagtg
     3841 cttcactgat acctcttatt aaatcagcat atcgaattcc ttttggtgaa ttttctaaaa
     3901 tttctcgtgc ttgttttatt attttttctc gttgggtaga cataattact cccaccaaat
     3961 taatggttta atcaatttat aatccaaatc ttttgtgttg attttcagcc catcttcaaa
     4021 ctctacctcg ccatcgctga tttttttaat ccatttaccg gtaagattag ataatgtttc
     4081 ggcaatgtct atgtttgagt agagtttatc taaatcaact ttgactttat tgttttcata
     4141 atctatttcc agcgctttaa gcatcttttc atcttttaga aagacatatt cctgatatgg
     4201 agttttcgaa ggtgaattaa ataaatcgct atcctcatac ttgcaatttg ccagtgcttc
     4261 ttcgtattgc tctaattcgt aatatttgaa aaatccgccg ccttgatatt cttttacttc
     4321 ctttgaaatt cctgatttat cataagccaa aacttttttc attcgtggta aaatcactgt
     4381 gtaaaaatgc tctcccatct ccacgccaag ccattttcta ccaagtttgt gggctactgc
     4441 tgttgttgtg ccggaaccga ggaagaagtc cattatgagc gattgttcat catattccat
     4501 tccctcaatc actcttttaa gcaatatctc agaattttca gtggcaaaaa aagtattgtg
     4561 ggtttcgtaa ctttttatgt cagtccagtt cgtatccaaa agtaattctg gttctttaag
     4621 atattcaggc attcctttaa cttcattatc ttgagtgtct ttatattttc ggccattatt
     4681 tattcttatc catcccaact tttcatactc actaattttt tgttgcgata atccccaatg
     4741 ttgccccttc ggaggcaacc aaggcttccc gaagaaatgt ctaacttgta attcataggt
     4801 ttttctttga cctggcatat ccaaagaaat ccacttacga tcttcttcac taatttttag
     4861 ttttgcgccc ttaaaagcta tcttgtttaa atcttttgca tagaagaaaa ttgaatcgat
     4921 gcctgtcaag tattgattag tcagcccttt cggcacatat ccccttttaa caaaaatctc
     4981 gtttctgaaa tttcccttac caaaaatttc atccattaaa tatctaacta tccaatttcc
     5041 attataatcg catcttacaa aaatgcttcc tttttcattc agccaatctt tagcaatctt
     5101 caatcggttt tctaaaagtg tagcccagtt ggcgttttta taatttgttc ggtataaaaa
     5161 ctgatctgat gaatctaaat taaacggcgg atcaatgtaa atcgtctgca ctttttcttt
     5221 gaatttcggt aggattgtat ttaatgcctg atagttttca ctctttatca gccagccgtc
     5281 caaagacttg tctaaatctt caaactgact caaaatctca agctctaaat ccttgaaata
     5341 tttggtatca atcggcaaat gttgatattt ttctgataaa tgcttactca ttaaatcgga
     5401 tgtaaaaatg tcaattactt tgaagctttc atctacaata ccaagttctt gccactcttt
     5461 aatttgttca tttatatttt tatgctttaa aattttttct actaatgata tatctttaat
     5521 tctatccagt gtaatcacat agtttgaatt tttgacaaac ttcggtttgt tccagatttt
     5581 taccagttca tcttcaaact gcgaaataaa atcaataatc ttaaaagcaa tgtcttttag
     5641 gatttgcaac tcgttgactc tatcggcact ccactcttta gctccctccc aaaaatactg
     5701 ataactccag agtttaaact gttcctgtaa aaacgctttg gcgtttttgt taataaagaa
     5761 atcaacctcg ctttgctttt caaaaaccct gaa
//
LOCUS       DAOSMC010000024         5475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_16306_length_5475_cov_20.483026, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000024 DAOSMC010000000
VERSION     DAOSMC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16306_length_5475_cov_20.483026"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PL075_02420"
     CDS             <1..789
                     /locus_tag="PL075_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOD97064.1"
                     /translation="VEDAALILETIAGKDSHDETSENFQFQAQSIYERKDLKGIKIGL
                     VKEIESNSFEKDLAISYQKSLDCLKQLGAAIEEISIPHISLSLPCYYVLMPAEVSSNL
                     ARFDGLRYSSSEKTGSLKDIYFETRGEYFGTETKRRIILGTFVLSSGYYDAYYLKARL
                     VQALIKKEFGEAFKKVDLLVWPTTPTPAFKFNEKLNDPLAMYLSDIFTVPANIAGIPA
                     VSLPIYGEGQLPYGLQFLASHFQEVKMLNVAYLFEQASNYGKFL"
     gene            773..1312
                     /locus_tag="PL075_02425"
     CDS             773..1312
                     /locus_tag="PL075_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97065.1"
                     /translation="MENFYNTLNYFLDQIDHFFSLPIVNKIKYIVLFLTLILIVIWVI
                     LLIRTNKIKDQVEKYKTFWMGRKHEKKHSVAVWRNIEHLMSAGDKESLKQAFLLMNEL
                     LTEVLNRIVSSGDNLIDKVKNLKIAEISESDKAKLLVGVYVYDKIKNDPTFNLESNEI
                     EFFFKLYRQIFVQLELLEE"
     gene            1314..3512
                     /locus_tag="PL075_02430"
     CDS             1314..3512
                     /locus_tag="PL075_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOD97066.1"
                     /translation="MVNKANKKEKNNKKEDLNGFSVQLKSETKKTIVGIICFGLAILF
                     VLSFFRLAGPFGYFLYDTLYYLFGWVYFFLPIILVGAGIIFISSFRRHLYLSTIIGLL
                     LITIGLLGISDLIKPLSAGLFGEGIGVLEQVFGFWGGIVFCLVVLVVGLVVAFEWSIK
                     LKREELKIVDKISKGMMPVEPVKNVTIKTGESEEKKNESKTKLANVIKEELDKAEKRK
                     AEKNNNFKKETFISKWNFPSLDLLERESGQPTSGDIKANVNIIKRTLANFGIPVEMGE
                     VYVGPTVTRYTLKPAEGVKLARIVALQNDLSLALAAHPIRIEAPIPGQSLVGIEVPNK
                     QKLIVRLRNFVESDRFLDSDLLVFPLGRDVAGEAVFVDLAKMPHLLVAGSTGSGKSIT
                     IHSLICSLLYKNTPETLRFLLIDPKRVELSVYNRLPHLISDVITNPKKTILALRWAVN
                     EMDERYNQLLASDVRDINGYNQKMMKQNKPLMPYLVIIIDELADLMVAYGREIEAAIV
                     RLAQMARATGIHLVVSTQRPSVEVVTGLIKANITSRIALQVASQVDSRTILDMAGAER
                     LLGRGDMLYLAGDTFKPKRIQGIFISESEVQKIVKYIIENNNNYEETIEESMDFEKEL
                     EKQMQEISLNESVGSDFEMDELYPEAYKLVVSSGKASASFLQRRLRVGYARAARLLDL
                     LEERGVIGPGEGAKPREVLIKSDTNAADISEEEYKDEIDASDETNEEVEE"
     gene            3540..4217
                     /locus_tag="PL075_02435"
     CDS             3540..4217
                     /locus_tag="PL075_02435"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOD97067.1"
                     /translation="MFGEFLSQKMEEKNFGVDDLARLTGIPEHYISALIEENFSHLPA
                     HVFTRGYLKKIAAVIDTDEEELWQFYLKEYNNLMPPPVDMLPTNRFESKGKTIAEILR
                     ALRYVPIWAIIITAIGFVLFQSKNLFGEPHLQVNNPVFENIETADNPFIIEGEGQPHT
                     YILLNGKEIYLSDDGKFSEPIQLKPGLNEIIVEATNRLGKKKTITRNIYFESTTTAPS
                     ASPNVSD"
     gene            4360..5253
                     /locus_tag="PL075_02440"
     CDS             4360..5253
                     /locus_tag="PL075_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97068.1"
                     /translation="MELSCFIGDEVFRTEDKLSQYLLGTDILVGPRSAIFPPTHLEGE
                     IRIGAENTIRASYLRNVKTEEECQIHYAQIFESELSNDVKVENAIMKESKLGAKTYVG
                     PGASLNRVITEERVKIPHVSYLSDLKINAQTNIAAGVITCNFDGVEKYSSVVGKKCFI
                     GVGVHLIAPCNLEDEVFVSEGVSIFPHTKIKQHSLVTGSGKQYKVHENRCFYVQSVGW
                     IITLSSVNPDLFQPIQKKIFEWKDFLKNQTLFDIKKWFLATNANLDFQKPIDYLRDPD
                     EKKMEKLIKLIEQKIKSSTIS"
     gene            5398..5470
                     /locus_tag="PL075_02445"
     tRNA            5398..5470
                     /locus_tag="PL075_02445"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5431..5433,aa:Ala,seq:ggc)
BASE COUNT         1797 a          801 c         1122 g         1755 t
ORIGIN      
        1 gttgaggatg ctgctttgat tttagaaacc attgctggca aagattctca tgatgaaaca
       61 agcgaaaatt ttcaatttca agcccaatca atttatgagc gaaaagatct gaaaggaata
      121 aaaattggtt tagttaaaga aatcgaaagc aattcgtttg aaaaagattt agccataagt
      181 tatcaaaaaa gtttggattg cttaaagcaa ttgggcgcgg cgatagaaga aatttctatt
      241 ccgcatattt ctttgagttt gccttgttat tatgttttga tgccagcgga ggttagtagt
      301 aatttagccc gctttgatgg cttgagatat tcatcatcag agaaaaccgg ctctttaaaa
      361 gatatttatt ttgaaacgcg cggtgaatat tttggcaccg agacgaaaag aagaattatt
      421 ttgggtactt ttgttttgtc gagcggatat tatgatgctt attatttaaa agcgcgtctc
      481 gtccaagctt taattaaaaa agaatttgga gaagctttta aaaaagttga tttgttagtt
      541 tggcctacta ctcctactcc agcttttaaa tttaacgaaa aattaaatga tccattagct
      601 atgtatttat ccgatatatt taccgtgccc gccaatatcg ctggcatacc ggccgtctct
      661 ttaccaattt atggcgaagg tcaattgccc tatggtttgc aattccttgc atcccatttt
      721 caagaagtta aaatgttaaa tgtcgcttat ttatttgaac aagcttcaaa ctatggaaaa
      781 tttttataat actttaaatt attttttaga tcagattgat cattttttta gtttgccaat
      841 tgtcaataaa ataaaataca ttgttttatt tttaaccttg attttaattg tgatctgggt
      901 aattttatta attagaacta ataaaattaa ggatcaagtt gaaaaatata aaactttttg
      961 gatgggtcgg aaacacgaaa aaaaacattc tgtagcggtc tggcgcaata ttgagcattt
     1021 gatgtctgcg ggcgataagg aatcattaaa acaagccttt cttttaatga acgaattact
     1081 caccgaagtt ttaaatagga ttgtttcgtc gggtgataat ttaattgata aggttaagaa
     1141 tttaaaaatt gctgagataa gcgaatcaga taaagcaaaa ttattagttg gcgtttatgt
     1201 ttatgacaaa attaaaaatg atccaacttt taatttagaa tcaaatgaga tagaattctt
     1261 ttttaaactc tatcggcaaa ttttcgttca actcgaactt ttagaagaat aatatggtta
     1321 ataaagctaa taaaaaggaa aaaaataaca aaaaagagga tttaaacggc ttttctgttc
     1381 aattgaaatc agagacgaaa aaaacaatag tgggtattat ttgttttggt ttggcgattt
     1441 tatttgtttt aagttttttc cgtttagcag ggcctttcgg atattttctt tacgatacgt
     1501 tatattatct ttttggatgg gtttattttt ttctgccgat tattctggtt ggcgctggta
     1561 ttatctttat tagttctttt cgcaggcatc tttatttatc aactattatt ggtctgcttt
     1621 tgattactat tggtcttttg ggcatttccg atctaattaa accactttct gctggtttat
     1681 ttggcgaagg gattggtgtc ctggagcagg tttttggatt ttggggaggc attgtttttt
     1741 gtttggtggt attagtggtg gggttagtgg tggcatttga gtggtctatt aaattgaaga
     1801 gagaagaatt aaaaatagta gataaaatat caaaaggaat gatgccggtg gagccggtga
     1861 agaacgtgac tattaagacg ggcgaatcag aagagaaaaa aaatgaatct aaaacaaaat
     1921 tagcaaatgt tataaaggag gaattggata aagcagaaaa acgcaaagca gaaaaaaata
     1981 ataattttaa aaaagagaca tttattagta aatggaattt tccatcactg gatctattgg
     2041 aacgagagag tggtcagcca accagcggag atattaaagc caatgtaaac attattaaaa
     2101 gaaccttagc gaattttgga attccagttg aaatgggtga ggtatatgta gggccgacag
     2161 tgacgcgtta tactttgaag ccagctgaag gcgttaagtt ggcaagaatt gtcgctctgc
     2221 aaaatgatct ttctttggct ttagctgccc atcctattcg aattgaagcg ccaattcctg
     2281 gccaatcact tgttggtatc gaagtgccca acaaacaaaa attaatagtt cgcttacgga
     2341 attttgttga atcggatcgt tttttagata gtgatttact agtttttcct ttaggtcgag
     2401 atgttgcggg agaagcagtc tttgttgatt tagccaagat gccgcattta ttggttgctg
     2461 gaagcacagg ttctggaaaa tcaatcacca ttcattcgct catctgtagt cttttataca
     2521 aaaacacacc tgaaacgctc agattccttt taattgatcc caaaagagta gagctgtctg
     2581 tttataatcg tctgcctcat cttatctctg atgttattac taatcctaaa aaaactattt
     2641 tagctcttag atgggcagtt aacgaaatgg atgagcgtta taatcaattg ttggctagtg
     2701 atgtgcgcga cattaatggc tataatcaaa agatgatgaa acaaaacaaa cccctgatgc
     2761 cttatttggt gattatcatt gatgaattgg ctgatttaat ggtggcttat ggcagggaaa
     2821 ttgaagcggc tatcgttcgt ttggctcaaa tggcgcgagc cactggtatt catctcgttg
     2881 tttctacgca gcgaccttca gtagaagtag tgacaggatt aatcaaagct aatattactt
     2941 cgcgcattgc tctgcaggtg gccagccaag ttgattcacg aactatttta gatatggcag
     3001 gagcggaacg gctattgggt cgaggcgata tgctttattt agctggtgat acctttaagc
     3061 caaagcgtat tcaaggtatt ttcatttctg aaagtgaagt gcaaaaaata gtgaaatata
     3121 ttattgaaaa caataacaat tatgaagaaa caatagaaga atcaatggat tttgaaaaag
     3181 aattagagaa acaaatgcag gaaatttctt taaacgaaag tgttggaagt gattttgaga
     3241 tggatgagct ttatcccgaa gcttataaat tggtagtgag tagcggcaag gcatcggctt
     3301 cgtttttaca gcgacgattg cgcgttggtt atgcgcgcgc tgctcgttta ttagatcttt
     3361 tagaagagcg aggagtgatt ggtcctggcg aaggagctaa accgagagaa gttttaatta
     3421 agtcggatac taatgcggct gatatttcag aggaagaata taaagatgaa attgacgcga
     3481 gtgatgagac taatgaagaa gtcgaagagt aattaataaa atgatttact aataatttaa
     3541 tgtttggaga atttttatcc caaaaaatgg aagagaaaaa ttttggcgtt gatgatctcg
     3601 ctcgattaac gggcattcca gagcattaca tcagcgcttt aatagaagaa aatttctctc
     3661 atttacctgc tcatgtcttt acaaggggtt atttaaaaaa aattgctgca gtgatagaca
     3721 ctgacgaaga agaattgtgg caattttatt taaaggaata taacaacttg atgccaccac
     3781 cagtagatat gctaccaact aatcgttttg aatcaaaagg caaaacgatt gcagaaatat
     3841 tgagagcttt gcgctatgtc cccatctggg ctattattat taccgcaata ggctttgttc
     3901 tttttcaaag taaaaatctt tttggcgaac ctcatttaca agttaataat ccggtttttg
     3961 aaaacataga aacagcagac aatccattta tcattgaagg cgaaggtcag ccgcatacct
     4021 atattctact taatggaaaa gaaatttatc tgagtgatga tggcaaattt tctgaaccga
     4081 ttcaattgaa acccggatta aatgaaataa ttgtcgaagc tacgaatcga ctaggcaaga
     4141 aaaaaacaat tacgagaaat atttattttg agtctacgac aacagcacca agcgcttctc
     4201 ctaacgtttc ggattagatt aattaggaat gttatcgtgc gggttagata taagacgggc
     4261 tggcaaattt tctttgaaaa tttgccagcc ttttattgac atttccgtct ataattttaa
     4321 actaaaaata gcaaattaat atattagaag gaggaaaaaa tggaactttc ttgttttatc
     4381 ggtgatgaag tttttagaac agaagataaa ttgagccaat atctgctagg gacagatatt
     4441 ttagtaggcc ctcgttcagc catttttcca ccgactcatt tggaaggtga aattcggatt
     4501 ggagcagaaa ataccatccg agcttcttat ttaagaaatg ttaaaaccga agaagaatgt
     4561 caaattcatt atgctcaaat ttttgaaagc gaattaagca atgatgttaa ggtagaaaat
     4621 gccattatga aagagagtaa gcttggcgct aaaacatatg taggaccagg cgcttctctt
     4681 aatcgggtga ttactgaaga acgagtaaaa atcccccatg tttcttattt gagcgatctt
     4741 aaaattaacg ctcaaactaa tattgctgcc ggtgtcatta cttgtaattt tgatggagta
     4801 gaaaaatata gttctgttgt aggaaaaaaa tgttttattg gtgtaggtgt tcatttgata
     4861 gcgccttgca acttagaaga tgaagtgttt gtaagtgaag gagttagtat tttcccccat
     4921 accaaaatta agcagcattc tttagtgact ggatcaggca aacagtataa agttcacgaa
     4981 aatcgctgtt tttatgtaca aagtgtggga tggatcatta ctctttcaag cgtcaatcca
     5041 gatctgtttc aacctattca gaagaagatt tttgaatgga aagatttttt gaaaaatcag
     5101 acattatttg atattaaaaa atggtttttg gcaacgaatg cgaatttgga ttttcaaaaa
     5161 ccgattgatt atttaagaga tccagatgag aagaaaatgg aaaaattaat taaattaata
     5221 gaacaaaaaa ttaaatcctc aactataagt tgaggatttt ttatttaaga cctatttttg
     5281 gcggatgtga gtgcggatta tataatccgc actcacagcc gcaccaacac cgaaagcaat
     5341 ttgatttttc caaagaaaaa gttaaaataa aacaaaagaa tgctattttc aaaaattggg
     5401 cctgtagctt agctggtaga gcgcctcgat ggcatcgagg aggtcagggg ttcgagtccc
     5461 ctcaggtcca ctata
//
LOCUS       DAOSMC010000025         5405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_16578_length_5405_cov_20.158692, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000025 DAOSMC010000000
VERSION     DAOSMC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16578_length_5405_cov_20.158692"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /gene="ssrA"
                     /locus_tag="PL075_02450"
     tmRNA           complement(<1..293)
                     /gene="ssrA"
                     /locus_tag="PL075_02450"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(297..743)
                     /gene="smpB"
                     /locus_tag="PL075_02455"
     CDS             complement(297..743)
                     /gene="smpB"
                     /locus_tag="PL075_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOD97069.1"
                     /translation="MQILVKNRKAQFNYEILEKFEAGIQLLGCEVKSIKAGNVSLDGA
                     IVQFYGNELYLINAFIPAWQPANAPANYKPDRSRKLLLHQKEIDYLRGKMKAERLMLI
                     PLTVYLKKNKIKLELGLVRSRKKIDKRELIRKRETQRELERKLKNY"
     gene            complement(745..2184)
                     /locus_tag="PL075_02460"
     CDS             complement(745..2184)
                     /locus_tag="PL075_02460"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOD97070.1"
                     /translation="MEKKDLVKINDYLWEIPKEFRSDMRVPARVYVSEKMLEEIFHDR
                     SLNQLVNITTLPGIANYSLAMPDIHEGYGFPIGGVAGIRANDGIISPGGVGYDINCGM
                     RILLSSYTETELNPYLKRLAEEIAMSVPSGLGRGHKRSINRIDLDKILNSGARELVQN
                     GYGEVDDLKYCESGGCLEMADARVVSNRAKNRGGDQVGTLGSGNHFLEIQKVVEIFDK
                     EVAKQFNLFPNQIVIMIHTGSRGLGHQVATDYIQIMMRAMSKYKINLPDRELACAPFN
                     SDEGQNYFQAMAAAANFAWANRQMISFYVRQAWEKILGKGSNLKILYDVAHNIAKLEE
                     YEVNGQRETLIVHRKGATRAFPAHHPEISEEYQSCGQPVLIPGSMGTASYVLVGVEKS
                     KETWHSTCHGAGRRMSRASATKNLSVQEILNNLKQKGVLVECNSLRGLAEEAPEAYKD
                     IDEVVNVVDQSGLSIKVAKLIPLAVIKGE"
     gene            complement(2365..3957)
                     /locus_tag="PL075_02465"
     CDS             complement(2365..3957)
                     /locus_tag="PL075_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD97071.1"
                     /translation="MGNQYYYLKVGKADELDNRKERRIFRFFEMLPGLCSWLTLILMV
                     IVSWQWPVVAAIFIIVFDTYWLIKTTYFSWHAAYSFKKVREHLKKNWLTELKNLSKES
                     YNININDWRDIYHLVIFPMVDESYAIVSSTFEALSSANYPLDRFIVVLATEERAQDKI
                     QSTIDKVKKEYGRNFKHFLITVHPKDIEGEIAGKGANETWAARQVKKLIIDQLNIPYE
                     HILVSTFDIDTVVPSDYFGCLTYHFLTAKNPLRSSYQPVPLFTNNIWEAPALARVISF
                     SSTFFQLIQQSRPEKMTTFSSHSMPFKALVDVDFWQTNVVSEDSRIFWQCFLYYNGDW
                     EVVPLFYPVSMDANVDVSFSKTMVNQYKQQRRWAYGALDIAYTFFGFIKNKRIPFKKK
                     LIYGFFLFDGFYSWAVTSILIFFLGWLPIVLGGPKFSFNILSFYLPKMTSYVLTVANI
                     GIITCAVLNFWFLPPRPPYYGKWRYLLFVLQWILTPINLIFFGSIPALDAQTRLLLKK
                     YMGFWVTPKYRKLEITQEKSGS"
     gene            complement(3975..4047)
                     /locus_tag="PL075_02470"
     tRNA            complement(3975..4047)
                     /locus_tag="PL075_02470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4012..4014),aa:Arg,seq:ccg)
     gene            complement(4105..5100)
                     /locus_tag="PL075_02475"
     CDS             complement(4105..5100)
                     /locus_tag="PL075_02475"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOD97072.1"
                     /translation="MSPIISLKNQPILKPRRLKKGDKVIVVSPSQPITNPKYFKRGLK
                     TLESLGFRVETSKHVNTVYGMYKAGTPAERAADLNEAFARKDIKGIFMSVGGFLANQV
                     LPLLDYKLIRHNPKVLIGFSDGTTLLNAIYRRSRIVTFHGFNIEHFFMKANSYTIKSF
                     QNICSEGGTTFLRRSKWQILKSGHVHGKLLGGNLVSFANLLGTRYFPDISNSILFFEE
                     HDDFSEDVEGALTRLINAGIFAEGGAQGIIFGKMINVTVGSADKEIQQKFTRPKFFTL
                     NTIIKELFKPYKIPIITNVDFGDIYYPMTIPLGIQAELDTTRSEITFRLLEPAVR"
BASE COUNT         1757 a         1089 c          878 g         1681 t
ORIGIN      
        1 tccagacaat aagccaattt gttttgagga ttaatattaa aattggcaaa actattaatc
       61 ccttgcctct gaatttggtc gcttttatga tcagaggcgc tcataaaagc ctagctcaat
      121 aatttgacac ccttctagcg tattgagcat cagctagtcg gatgccggct taagctaaag
      181 cgaaagccag attggaattt gaattggcaa tttttgttgc ccgatgatta acgaggtaac
      241 ggttcctcga tctgactcga aaatattttt tcgcttcgag gccaatcatc cccgaattaa
      301 taatttttaa gcttcctttc taattccctc tgcgtctctc tttttctaat aagctctctt
      361 ttgtcaattt tttttctact tcttaccaat cctaattcta acttaatttt attttttttc
      421 aagtaaaccg tcagaggaat aagcattaaa cgttcggctt tcattttacc ccttaaataa
      481 tcaatttctt tctgatgcaa aagcaatttg cgactgcgat ccggcttata atttgctggt
      541 gcattagcag gttgccaggc cgggataaaa gcattaatta aatacaactc attgccgtaa
      601 aattgaacaa tagccccgtc taaagaaaca ttgcctgcct ttattgactt aacttcgcaa
      661 cccaaaagtt gaatacctgc ttcaaatttt tctaaaattt cataattaaa ttgggctttt
      721 cgatttttta ctaaaatttg catattattc tcctttaata actgctaatg gtattagttt
      781 ggctacctta attgacaatc cactttgatc aacaacatta acgacttcat caatatcttt
      841 atatgcctca ggcgcttctt ctgccaatcc tcttaaacta ttacattcca caagaacacc
      901 tttttgtttc agattattaa gaatttcttg aacagataaa tttttagtag cggaggcgcg
      961 cgacatacgt cgaccagcgc catgacaagt tgaatgccat gtctctttgc ttttttctac
     1021 tcccactaaa acgtacgagg cagttcccat gctgccaggg ataagcacgg gttgcccgca
     1081 agattgatat tcttcagaaa tttccggatg atgagcagga aaagcgcgtg tcgctccctt
     1141 acgatgaaca ataagagttt ccctctgacc gttaacctca tactcttcca actttgcaat
     1201 attatgagct acgtcataaa gaattttaag gttactacct ttgcctaaaa ttttttccca
     1261 cgcctgtcga acatagaaac ttatcatttg acgattcgcc caagcaaaat tagctgctgc
     1321 ggccatagct tgaaaatagt tctggccttc atcgctatta aaaggagcac atgccaattc
     1381 ccgatcaggc aaattaatct tatattttga cattgcccgc atcataatct gaatataatc
     1441 ggtagccact tgatgaccta ggcctcgcga gcctgtatga atcattatca caatttgatt
     1501 tggaaaaaga ttgaattgtt tagcaacttc cttatcaaaa atttctacca ctttttgaat
     1561 ttctaaaaaa tggttgccgc taccgagcgt acctacttga tcacctcctc gatttttagc
     1621 gcgattagaa acaacacgag cgtcagccat ttctaagcaa cctcctgatt cgcaatattt
     1681 taaatcatct acctcgccat aaccattttg caccaattct cttgctccac tatttaatat
     1741 cttgtctaaa tcaattcgat taatacttct tttgtgccct ctgcccaaac ctgaaggaac
     1801 gctcatggca atttcctcag ctaaacgttt caaataagga ttaagctctg tttcggtata
     1861 agaagataac aaaattctca tcccacaatt aatatcgtaa cctactccac ctggtgaaat
     1921 tatgccatca ttggctctaa tacccgctac gccaccaatg ggaaagccgt agccttcatg
     1981 aatatctggc atagctaaac tatagtttgc aatgcccgga agagtagtaa tgtttactaa
     2041 ttgattaagg gaacgatcat gaaaaatttc ttccaacatt ttttcagaaa cataaacacg
     2101 cgctggcacg cgcatatccg atcgaaattc tttaggaatt tcccagagat aatcgtttat
     2161 ttttaccaaa tcttttttct ccataaaatt ttgagttaca attaaattat aataacaata
     2221 aattcaaatc tcaaatctgc gagtagtcat ttgtgcggaa aaatttcgtt gccaataggc
     2281 ggacgaaggg ggcggcggcg atttttgtaa aaaaatcgcc gccgcgagac attttccaac
     2341 tctataaatc tataaaaatt attattaact gccggatttt tcttgcgtta tttctaattt
     2401 gcgatattta ggcgtaaccc aaaatcccat atattttttc aaaagtaatc gcgtttgggc
     2461 atcaagagca ggaatgctgc caaagaagat caaattaatt ggtgtcaaaa tccattgtaa
     2521 aacaaataat aaataacgcc attttccata ataaggtggc cgcggcggca aaaaccagaa
     2581 atttagtaca gcgcaggtga taatgccgat attagctacg gtcaaaacat acgaagtcat
     2641 tttgggtaaa tagaaagaca ggatattaaa actaaatttc ggcccgccca aaacaattgg
     2701 caaccaaccg agaaagaaga ttaaaatact tgttactgcc caactgtaaa agccatcaaa
     2761 aagaaaaaat ccgtaaatga gtttcttttt gaaaggaatt cttttgtttt taataaagcc
     2821 aaagaaagtg taagcgatat caagcgcgcc gtaagcccag cgtcgctgct gtttgtattg
     2881 attaaccatt gttttgctga aactcacatc aacattggca tccattgata ctggataaaa
     2941 taacggcacc acttcccaat cgccattata atacagaaaa cactgccaaa aaattcgcga
     3001 atcttcagaa actacattcg tttgccaaaa atctacatca accaaagctt taaatggcat
     3061 tgagtgagaa gaaaaagtcg tcattttctc tggtcttgat tgttgaataa gttgaaagaa
     3121 tgttgaagaa aaagaaatca ctcgagctaa tgccggcgct tcccaaatat tattagtaaa
     3181 tagaggtacg ggttgatagc ttgaacgcaa aggattttta gccgtgagaa aatggtaagt
     3241 tagacaacca aaataatcgc taggaacaac agtatcaata tcaaatgtgg aaactaaaat
     3301 atgttcatag ggaatattta actgatcaat aatgagtttt ttaacttgtc gagctgccca
     3361 agtttcgtta gcaccttttc cagctatctc accttcaata tctttaggat gaaccgtaat
     3421 taaaaaatgt ttgaaattcc tgccatattc ttttttaact ttatcaattg ttgattgaat
     3481 tttatcttga gcacgttcct ctgtggctaa aactactata aatcgatcta aaggatagtt
     3541 cgcagaactt aatgcttcaa aagtagaaga aacaatggca taagattcat caaccatcgg
     3601 aaagattact aaatgataaa tatctctcca gtcattgatg ttaatattat agctttcctt
     3661 tgataggttt tttaactctg ttaaccaatt tttctttaaa tgctctctaa cttttttaaa
     3721 actataagct gcatgccaac taaaatatgt agttttaata agccaataag tgtcaaaaac
     3781 aataataaaa atagccgcta cgaccggcca ctgccaagaa actataacca ttaaaatcaa
     3841 tgtgagccag gagcaaagac ctggcaacat ttcaaaaaaa cgaaaaatcc gacgctcttt
     3901 gcgattatcg agttcatctg cttttcctac tttaagataa taatactggt tccccataag
     3961 tcggcgatta aaagtgcgcc tggcgcgact tgaacgcgca atcttcggct ccggaggccg
     4021 acgctttatc cattaagcta caggcgcact tttaatcatc gtttcaataa tagtttacct
     4081 ctgatttgct tagaaacaat accactatcg aacagctggt tcaagtaatc gaaaagtgat
     4141 ttcggatcta gtagtatcga gctctgcttg aatacccaaa ggaatagtca ttggatagta
     4201 aatatcacca aaatctacat ttgtaatgat aggaatttta taaggtttaa ataattcttt
     4261 gatgatggta tttaaagtaa aaaacttcgg ccgggtaaat ttttgctgga tctctttatc
     4321 agctgatcca actgtaacat taatcatttt accaaaaata attccttgag cacctccttc
     4381 ggcaaaaatt cccgcattga tcaaacgcgt taaagcacct tctacatctt ctgaaaaatc
     4441 atcatgttct tcaaaaaata aaatactgtt ggaaatatca ggaaaataac gcgtacccaa
     4501 aagattggca aaactaacca aattgccacc taataattta ccatgaacat gaccggattt
     4561 taagatttgc catttactac gtcgcaaaaa cgttgttcct ccttcagaac aaatattttg
     4621 gaaagattta atcgtataac tattagcctt cataaaaaaa tgctcaatgt taaaaccatg
     4681 aaaagttacg atgcgactcc gacgataaat tgcgttaagc agtgtagtgc catcggaaaa
     4741 accaattaat actttagggt tgtggcgaat taatttataa tctaataacg gaagaacttg
     4801 atttgccaaa aaccctccga cactcataaa aatgcctttg atatctttgc gagcaaaagc
     4861 ttcatttaaa tcagctgctc tttcagccgg agtccccgct ttatacattc cataaacagt
     4921 attcacgtgt ttggaagttt caacgcgaaa accaaggctt tctaaagttt taagtcctct
     4981 cttaaaatat ttcggattag taatcggctg agaaggcgaa acaacaataa ccttatcgcc
     5041 ctttttaagc ctacgtggtt ttaatatcgg ttgatttttt aaactaataa ttgggctcat
     5101 ggtaataaat aataaaaaag gctgctatca gctcttttcc cgttttgttg aattatactc
     5161 aaaaattgat tttaagcaag aaagacaata aaaaatcctg ctattgtaag caaatgaaaa
     5221 aagtgcataa aatatacgca aaattgctct gtcgtgctaa tttttttatt cgttttgact
     5281 tgctctgtct atttgccaag aaataaaaga ccgggagtct catacatata tcagtaattt
     5341 acttatttga ttgaatcatt tttagtgggc gcactaggga tcgaacctag gacctgttgt
     5401 ttata
//
LOCUS       DAOSMC010000026         5089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_17881_length_5089_cov_16.980930, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000026 DAOSMC010000000
VERSION     DAOSMC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17881_length_5089_cov_16.980930"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1908
                     /locus_tag="PL075_02480"
     CDS             109..1908
                     /locus_tag="PL075_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOD97073.1"
                     /translation="MANFPFFQKNNENINIPQVSKTGPQTIAEILTPPALEIESNYLR
                     LGDLYVKTFYIISYPRYLTTGWFSPILNIDQTVDISVFIHPIDSGTALKKLRRKVAQV
                     EAQIMEKEEKGVVRDPILETAYQDLENLRDALQQAREKLFEVGVYIAIYASNLQELTT
                     IEQKVINFLEGRMVYIKPALFQQAEGLQSCIPLNLDLLSVHSTLNSSPVSTIFPFISL
                     DLTSDKGVLYGINRHNNSLIIFDRFSLENGNMVIFAKAGGGKSYFCKLEVLRSLMMGT
                     DIIIIDPEKEYEHLAETVGGSFINISLTSEHHINPFDLPPARVDESPAEVLKSNILNL
                     TGLLRVMLGDLTAEQVGILDRALSETYASRDITPESDFSKLTPPLMEDLQSVLENMEG
                     GAELADRLYKFTRGTFAGFLNQPTNIDINNRLVVFGIRDLEDELRPIAMYIVLNFIWN
                     IIRAQMKKRILVVDEAWWMMKYPAGADFLFGLVKRCRKYFMGVTTITQDVEDFIRSPQ
                     GKPIITNSALQLLLKQSPASIEVVGETFNLTEAEKQLLLESNVGEGIFFAGSKHVAIK
                     IIASYAEDQIITTNPMEMMEIEKAKKEFDQSLK"
     gene            1938..2372
                     /locus_tag="PL075_02485"
     CDS             1938..2372
                     /locus_tag="PL075_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97074.1"
                     /translation="MNEKENLQLNQDEMIDDELLDEEGGEGEGETKSRSKISLIELLI
                     FPLPFAIIADLADFISWSGVGTLISWVLDIVTAGGLGLWLFLKGARGEYMVVTGLIEM
                     IPFVDFLPLKTTVLIWLYVKQKNPALGKITETMGKASVKKSS"
     gene            2374..3090
                     /locus_tag="PL075_02490"
     CDS             2374..3090
                     /locus_tag="PL075_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97075.1"
                     /translation="MKNKKLLLIIFLFGLIFVAKDLVLAAEKTPQSFLYLTWESDGMA
                     PLTYKAKAMPAEFAMIKVSVQPLIYSKGSYLNSDNWNYQWFVNEELINQGVGLKEVRF
                     IAKDYTRADYEVTVRVFSTVSETPFIKTATINLVKPLVYLKPVNEKSIALNSYNTSEN
                     LVELEAVPFFFGSYGLNQLHYSWAINGYPIADFDNEKSIMVPNIGEENPFSTNIKLTV
                     EDQRDVILRAESALNINFIK"
     gene            3087..3764
                     /locus_tag="PL075_02495"
     CDS             3087..3764
                     /locus_tag="PL075_02495"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HOD97076.1"
                     /translation="MKKSLAFIFLFVSFLAVPTALIFAQSTSSTSTGSLLGPKEGQGG
                     CMEIELPYIPRCGATPASYLSGLYRLALVLGVLAALIMIVIAGITYATSGDNASKQKE
                     ARGQIKDAIIGMVILFASVLILNTVNPALVSMRLPNIQPPSTPAPTSTSTSSEPSDSG
                     SLKDRWKRAVAEASVVECMMGSGTSGKGGCVDRACSQFKDNTKFQLCSKACQIYCEQK
                     YHIHIRE"
     gene            3767..5032
                     /locus_tag="PL075_02500"
     CDS             3767..5032
                     /locus_tag="PL075_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97077.1"
                     /translation="MKKNVIITIIIIIAIALIAAAFYWFYLKPQTQIEEGILPEGGGI
                     LPTISVSPSPTGQQGSVETGGVAQPILKALINKDILAFWPSATSSLQYLSQEGLFEIN
                     YSEFPIKEEKKDLGVNFSNILSVDPSAKGKVLIKYVATGATKPAYSILDIKTGEIKNL
                     DTYIQSAAWSPDGETLIYYYSNSPLYYQENFKESSYLAQLDKNLANRKILMNFKAASD
                     INLLWPATNTVYILQKPSGLAESTILTFDVKNKVFAPFVSGNGLMLQWDKQGKYGLLF
                     SVEPNTQKPKLELINSDGMVLAEIPNVTLPEKCVFAGSKPVVYCSIFVNPTFRSIWPD
                     VYYIGTVSFDEMIYEIDLETMEAKLLNNTIFKVENIKISPDDKYLLFFDDKTRTLYSL
                     SLPNMEEQPASSTTPTPYSTTTNSVSPTP"
BASE COUNT         1605 a          820 c         1013 g         1651 t
ORIGIN      
        1 gtcgaattat actataactt ctataatcct gaaaaacagg aaagaaaaaa tctagcaatt
       61 gcatcagaat tagacgaaga atacgaaaat aattcttaat ttaaaaatat ggctaatttt
      121 cctttttttc aaaaaaataa tgaaaatatt aacattcccc aagtttccaa aactggccct
      181 cagacaatag cggaaatttt aacgccgcct gctttggaaa tagaatcgaa ttatctacgc
      241 cttggtgatt tgtacgttaa gactttttat attatttctt atccgcgtta tcttacgaca
      301 ggttggtttt cgcctatttt gaacattgat caaacggtgg acatttcggt ttttattcat
      361 ccgattgata gtggcactgc tttaaaaaaa ttacgacgga aagtagctca agtagaagcg
      421 caaattatgg aaaaagaaga aaagggagta gtaagagatc ctattttaga gacagcttat
      481 caagatttag aaaatttgcg cgatgcttta caacaagcaa gagaaaaatt atttgaagtg
      541 ggtgtttata ttgctattta tgcttctaat cttcaagaat taaccacaat tgagcaaaaa
      601 gtgatcaatt ttttagaagg ccgaatggtt tatatcaaac ccgctctttt tcaacaagct
      661 gaagggttgc aatcttgtat tcctttaaat cttgacctct taagcgttca ttcaacgcta
      721 aattcatcgc ctgtttcaac aatttttccc tttatttctt tggatttaac ttctgacaaa
      781 ggagtgcttt atggcattaa tcggcacaat aatagtttaa ttatttttga tcgtttttct
      841 ttagaaaacg gcaatatggt tatttttgcc aaagcgggcg gtggtaaatc gtatttttgt
      901 aaattagaag ttctacgttc tttaatgatg ggtacggata ttattattat tgatccagaa
      961 aaagagtatg agcatttagc cgaaaccgta ggtggatctt ttattaatat ttctttaaca
     1021 tccgaacatc atattaatcc ttttgattta ccgccggcgc gggttgatga gtcgccggct
     1081 gaagtattga aatcaaacat tctaaattta accggattgt taagagtaat gctcggagac
     1141 ttaaccgccg aacaggttgg aattttagat cgggcgcttt ccgaaacata tgcttcaaga
     1201 gatattacgc cagagagcga tttttctaaa ctgacgccac ctcttatgga agatttgcaa
     1261 tcggttttag agaatatgga aggtggcgca gaattggccg acagactata taaatttacg
     1321 cgaggtactt ttgctggatt tttaaatcag cctaccaata ttgatatcaa taatcgttta
     1381 gtcgtttttg gaattagaga tttagaagac gagttgaggc ctatcgcgat gtatatagtt
     1441 ttaaacttta tttggaatat tattcgcgcg cagatgaaaa agagaatttt ggtggttgac
     1501 gaggcgtggt ggatgatgaa atatccagca ggcgctgatt ttcttttcgg tttagtaaaa
     1561 agatgcagaa aatattttat gggtgtgacg accattactc aagatgtgga ggattttatt
     1621 cgttcaccac aaggaaagcc aattattacc aattcagcct tacaattact tttgaaacaa
     1681 tcccctgctt ctattgaagt agtaggcgag acgtttaact taaccgaagc cgaaaagcaa
     1741 cttcttttag aatcaaacgt aggagaaggc atcttttttg ctggttcaaa acatgtggct
     1801 atcaaaatca tcgcttctta cgccgaagat caaattatta caactaatcc aatggaaatg
     1861 atggaaatcg aaaaagcaaa aaaggagttt gatcaaagtc taaaataatt gcttcttgtt
     1921 taaataaatt ttataatatg aatgagaagg aaaatttaca attaaatcaa gatgaaatga
     1981 tagatgatga actgctagat gaggaagggg gtgagggaga gggagaaacg aaatcaaggt
     2041 ctaaaatttc tttaattgaa ttgctgattt ttccactgcc ttttgcaatt attgctgacc
     2101 tcgctgattt tattagctgg agtggcgtgg ggacattaat tagctgggtt ttggatattg
     2161 tgacagctgg aggattaggg ttgtggttat ttcttaaagg ggcacgcggt gaatatatgg
     2221 ttgtaacagg gctaattgaa atgatacctt ttgttgactt tttaccatta aaaacaacag
     2281 ttttgatttg gctgtatgta aagcaaaaaa atcccgcgtt aggcaaaata accgaaacaa
     2341 tgggaaaagc aagtgttaaa aaatcaagtt aatatgaaaa ataaaaaact tcttttgata
     2401 atatttttat tcggtttgat ctttgttgca aaggatcttg ttttggctgc tgaaaaaact
     2461 cctcaaagtt ttttatatct aacatgggaa tcagatggga tggcgccttt aacttataaa
     2521 gcaaaagcca tgcctgcaga attcgcgatg atcaaagttt ctgttcaacc tttaatatat
     2581 tctaaaggca gttatttaaa ttcggataac tggaattatc aatggtttgt aaatgaagag
     2641 cttattaatc aaggggtggg attaaaggaa gtgcggttta ttgccaaaga ttatacacgc
     2701 gcagactatg aagtaacggt gagagtgttt tccactgttt ctgaaactcc atttattaaa
     2761 accgcaacga ttaatttagt gaaaccactc gtttatttaa agcccgtgaa cgaaaagtcc
     2821 attgctctta attcgtataa tacctcagaa aatcttgttg aattagaagc ggtgccattc
     2881 ttttttggca gctacggttt aaatcaactt cattatagct gggcgattaa tggttaccct
     2941 attgctgatt ttgataatga aaaaagcatt atggtgccta atattggtga agagaatcct
     3001 ttttcaacta atatcaaatt aacagtagag gatcaaagag atgtgatttt aagagccgag
     3061 tcggcgctta atattaattt tatcaaatga aaaaatcttt agcctttatt tttctgttcg
     3121 tttcattttt agccgttccc acagcattaa tttttgctca atcaacctct tctacttcca
     3181 cggggtcttt actgggtcct aaggaggggc aaggagggtg tatggaaatt gagttgccat
     3241 atattcctcg atgtggtgct actccagcct cctatttgtc tggattatat cgattggctt
     3301 tggttttggg agttttggct gctttgatta tgattgtgat agccggaatt acttatgcca
     3361 cgagtggtga taatgcgagc aaacagaaag aagcgcgagg tcaaattaag gatgcgatta
     3421 ttggtatggt gattcttttt gcttcggtat taattcttaa tactgtcaat ccagctcttg
     3481 taagtatgcg tttgccgaat attcagcctc cttcaactcc tgcgcctacg tcgacttcaa
     3541 cgtcttcaga gccttcagat tctggttctc tgaaggatag atggaagaga gctgtagctg
     3601 aagcttccgt tgtagaatgt atgatgggaa gtggtacgag cgggaagggc ggttgcgtgg
     3661 atagagcttg tagtcagttc aaggacaaca caaaatttca gttgtgctct aaagcctgtc
     3721 agatctattg cgagcagaaa tatcatattc atattaggga ataactatga aaaaaaacgt
     3781 tattatcact attattatca ttattgccat tgcgcttatt gctgcggcat tttactggtt
     3841 ttatcttaag ccgcaaacgc aaattgagga aggcattctg cctgaaggcg gaggaatttt
     3901 gccaaccata agcgtttcgc cttcgccaac agggcaacaa ggatctgtag aaaccggcgg
     3961 cgttgctcaa cctattttaa aagcgctgat taataaagat attttagctt tttggccaag
     4021 cgccacttcg tctttgcaat acctcagcca agaaggattg tttgaaatta attattcaga
     4081 atttcctatt aaggaagaaa aaaaggattt aggagttaat ttttctaata ttttgtctgt
     4141 tgacccttca gctaaaggga aagttttaat taaatatgta gccacaggag cgacaaaacc
     4201 ggcttatagt attttagata ttaaaactgg agaaataaaa aacttggata cctatattca
     4261 aagcgctgct tggtcacctg atggcgaaac cctcatttac tactattcta atagcccgct
     4321 ttattatcag gagaatttta aagaatcaag ctatctagct caattagata aaaacttagc
     4381 caatcgcaaa attttaatga attttaaagc agccagcgat attaatttat tatggccagc
     4441 gactaatact gtttatattt tgcaaaaacc gagtggatta gctgaatcaa ctattttaac
     4501 gtttgatgta aaaaataaag tttttgcgcc ttttgttagt ggcaatgggt taatgcttca
     4561 atgggataaa cagggcaaat atggcttgct ttttagcgtt gaaccgaata cgcaaaagcc
     4621 aaaattagaa ttgattaata gcgatggaat ggttttggca gaaattccca atgttacttt
     4681 gccggagaaa tgtgtttttg ccggaagtaa accggtagtt tattgcagca tctttgttaa
     4741 tcctaccttt agaagcattt ggcctgatgt ttattatatt ggtacggttt cttttgatga
     4801 gatgatttac gaaattgatt tagaaacgat ggaagctaaa ttattgaata atacaatttt
     4861 taaagttgaa aatattaaaa ttagcccaga tgataaatac ttattatttt ttgatgataa
     4921 aaccagaacc ctttatagtt taagcctacc gaatatggaa gaacaaccag catcctcaac
     4981 aacaccgact ccatattcaa cgactactaa ttcagttagc cccactccct aaccgtttat
     5041 tcaatagaaa tttactttgt attttatttt cagccaaaaa ttgcctctt
//
LOCUS       DAOSMC010000027         5023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_18179_length_5023_cov_21.430556, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000027 DAOSMC010000000
VERSION     DAOSMC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18179_length_5023_cov_21.430556"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..512)
                     /locus_tag="PL075_02505"
     CDS             complement(156..512)
                     /locus_tag="PL075_02505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOD97078.1"
                     /translation="MIKKFIKSVSYAWAGIKEAWRGQSFRIMLVLALLAIGLGFGLHI
                     SLLEWVLLILSVGFVLALEIINTAWELSLDYLRPEINPLAGKIKDLSAGAVLVASITA
                     LIIGLIIFLPKILEFL"
     gene            complement(523..1929)
                     /locus_tag="PL075_02510"
     CDS             complement(523..1929)
                     /locus_tag="PL075_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOD97079.1"
                     /translation="MKKVLVFLQQEWVLILILLVLASVTRFLYLGHPNQTVFDEVYFA
                     KWVTNYYDGEFYFDIHPPLAKMMLATLAKIFGYADAGNTNFSQIGNEYQTDFYKFLRG
                     VESFFGVVLVLAVYALGKKMFKNKWPGFLAALLVTFDNAILVQSRFIFTDVFLLAFGV
                     IGLYFVYRLKDHQKIDSESFVDLFFAALFLAGSILIKWTGLIFVGVGFFVLLLDRLRN
                     KKNFTLFLKEAVTIGVIIVLVYFGIFAAHLLSLPYSGGSNADGFLSVRSQSALLGNQY
                     YGQYQPPNLVSRIVELNIAMYKANASINQPHPYASKWYSWPIMARPIFDWQEALEGGQ
                     KTARIYLLGNPLIWWLGLLCVVFIFFVLIDELRFKKKTIYFEPLLVLMIGYIFNLLPY
                     VLITRPAFLYHYFPSFIFMTLMSGFILWFFFKNKPVVLVLIFLVIIQSFIYFAPLSYG
                     LPLTDNQFEARMWFNNWK"
     gene            complement(1926..2063)
                     /locus_tag="PL075_02515"
     CDS             complement(1926..2063)
                     /locus_tag="PL075_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD97080.1"
                     /translation="MPYRGKRNEPTFVEFVAQKLAVIKNLSVEKVAEATVNNCFNLFN
                     L"
     assembly_gap    2076..2175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2176..2570)
                     /locus_tag="PL075_02520"
     CDS             complement(<2176..2570)
                     /locus_tag="PL075_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOD97081.1"
                     /translation="MKPYLIDSHAHLQFDIYNNDREAILKRMKEKNIWAINIGTNLNL
                     SRKAIDLAKQYDFLFAVIGLHPIHLFESLDPKEGQTEPEIFEVEKFHLLAQEKKVVGV
                     GECGLDYFHNNDEAEKAKQKEEFKKQAVFA"
     gene            complement(2572..4053)
                     /gene="metG"
                     /locus_tag="PL075_02525"
     CDS             complement(2572..4053)
                     /gene="metG"
                     /locus_tag="PL075_02525"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOD97082.1"
                     /translation="MLKKIYITTSIPYVNANPHLGHALEFVQADCAARYERLQGNDVF
                     FATGVDENSLKNALAAEKAGRNVKEFVDEKAQVFKDFLKLLNIEISDFIRTTEPRHLE
                     GVKKFWLAAQKDIYQSEYEGLYCVGCEDFLKEEELVDGLCPIHHTAPQRIKEENYFFR
                     LSRFQKQLEEIYQNDLIQIIPPNRKQEVVSFIESGLKDFSISRSQERAHNWGISVPND
                     NSQVIYVWFDALCNYITVLGYGVNSPLYRAWWEDPSTEVWHFLGKDIAKFHCIYWPAM
                     LLAAGVRLPNKIAIHGFITLEGDKMSKSKGNVIDPRELIKEFGVDSLRYYLLREFSLS
                     QDGDFSVDNLKERYNKELANGLGNLLNRVVALIEKQGGKIKIEKNLMAKEIEECKEKY
                     NAALEDFRFNQGAEAFLQLISQADAFINQNQVWAVTDLAKKEELLSSLWLTLANVADF
                     VYPYIPEASAKIKESLGVHNEKDFFGKEFLVKRLPPLFPKLNF"
     gene            4082..4618
                     /locus_tag="PL075_02530"
     CDS             4082..4618
                     /locus_tag="PL075_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97083.1"
                     /translation="MQIISVLLTPHFLTGAAIVKIVPNKPLAYILAFFAHFILDRIPH
                     SDHFLSGISSGRKNKSFYQTIGPIIFDFFIGITLIIIAYFTFDNFALTPVLIGGFLGI
                     LPDVFNALSYFNYKKNYLLLFKGQPLYPVSEFITQTKNPYYRFHRFFHNEKVYNGILG
                     TITQLAFVVVDLIVLLVL"
     gene            complement(4692..>5023)
                     /locus_tag="PL075_02535"
     CDS             complement(4692..>5023)
                     /locus_tag="PL075_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOD97084.1"
                     /translation="CELVPMVGPILAGVVATIVALTQSLPLAIWTAIAFILIQRLEND
                     ILVPAIMGKTIGFNPVIVLVALFAGGKLGGVLGMLIALPVVIVVEEIIKELNKKKQQR
                     LEVAPLP"
BASE COUNT         1707 a         1032 c          686 g         1498 t
ORIGIN      
        1 cctaccggca ggcaggtacc aattgagtta cggggggatt attaaaatta taaattaaaa
       61 ggcaattaaa aaattctctc gaaaattttt ggcattaatt ctaatgtttg aataatgcct
      121 ttaatctata atttaatttt aatcttattc tttttttaca aaaattcaag aattttggga
      181 agaaaaataa ttagacctat tatcagagct gtaatcgacg ctaccaaaac tgcaccagca
      241 gataaatctt taattttccc agccaaagga ttaatttcgg gccttaaata atccaaactc
      301 aattcccaag cagtattaat aatttccaaa gctaacacaa atccaacact taaaattaaa
      361 agcacccatt ccaacagcga aatatgtaat ccaaaaccga gtccaatcgc caacagtgct
      421 aaaactagca taatccgaaa actttgccca cgccaagctt cttttattcc tgcccacgca
      481 taagatacgc ttttgataaa tttttttatc ataatttatt atttatttcc agttattaaa
      541 ccacatgcgc gcctcgaatt gattatccgt taaaggcagg ccataactta aaggtgcaaa
      601 ataaataaaa gattgaataa tcaccaaaaa aattaaaacc aaaaccacgg gtttattttt
      661 gaaaaagaac cacaaaataa atccactcat taaagtcata aaaataaaag atggaaaata
      721 atgataaaga aaagcaggcc gtgttatcaa aacgtaaggc aaaagattaa aaatatagcc
      781 gatcattaaa actaacagcg gctcaaaata aatggttttc tttttgaagc gcaattcatc
      841 aatcagtaca aagaaaataa aaacaacgca aagcaatccc agccaccaaa ttaaaggatt
      901 gcctaaaaga taaatgcgag ctgttttttg tccaccttcc aaggcctctt gccaatcaaa
      961 aatcggacgc gccataattg gccagctata ccactttgag gcgtaaggat gcggctgatt
     1021 aatactagca ttggccttat acatcgctat atttaattcc acaatgcggc taaccaaatt
     1081 aggcggttga tattgtccat aatattgatt acccaacaag gcgctttgag aacgaacgga
     1141 taaaaaacca tcggcattag aaccgccact gtagggcaag cttaataaat gagcggcaaa
     1201 aataccaaaa taaaccaaaa caataattac acctattgtc accgcttctt tcaaaaacaa
     1261 agtaaaattt tttttatttc ttaatctatc caaaagcaaa acaaaaaatc ccacccctac
     1321 aaaaatcaat cctgtccatt taattaaaat actgccagct aaaaataaag ccgcgaaaaa
     1381 taaatccaca aaagattcag agtcaatttt ttgatgatct tttaatcgat aaacaaaata
     1441 aaggccaatc acaccgaaag ccaaaagaaa aacatcagta aaaataaaac gcgattggac
     1501 taaaattgca ttatcaaaag tcactaaaag cgctgctaaa aatcccggcc atttattttt
     1561 aaacattttt ttacctaatg cataaacagc caaaactaaa acgacgccaa aaaatgattc
     1621 cactcctctc aaaaatttgt aaaaatcagt ctgatattca ttaccgattt gtgaaaaatt
     1681 cgtattgcca gcatcagcat aaccaaaaat tttagctaaa gtcgccagca tcattttggc
     1741 caaaggcgga tgaatatcaa aataaaattc cccgtcatag taattggtta cccatttggc
     1801 aaaataaacc tcatcaaaaa ccgtctgatt aggatggcct aaatataaaa atcgcgttac
     1861 acttgccaaa accaaaagaa ttaaaatcaa aacccattct tgttgcaaaa aaactaaaac
     1921 ttttttcata aattaaaaag attaaaacaa ttatttactg ttgcctcggc cactttttca
     1981 acggataaat tttttataac tgctaatttt tgtgcgacaa attccacaaa agttggttcg
     2041 tttcttttcc cccgataagg caccggcgtt gcatannnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnngcaaa cactgcttgt tttttaaatt cttctttttg cttcgctttc
     2221 tcagcctcat cattattatg aaaataatcc aaaccgcact ctcccactcc caccaccttt
     2281 ttttcttgag ctaataaatg aaatttttca acttcaaaaa tttcgggctc ggtttgcccc
     2341 tctttaggat caagggattc aaacaaatga atcgggtgta aacctatcac agcaaaaaga
     2401 aaatcgtatt gtttggccaa atcaatcgcc ttccgtgata aatttaaatt cgtaccaata
     2461 ttaatggccc aaatattttt ttctttcatt cgtttcaaaa tagcctcgcg atcgttatta
     2521 taaatatcaa attggagatg ggcgtgcgaa tcaataagat atggcttcat attaaaaatt
     2581 taacttagga aaaagcggcg gcaaacgttt aaccaaaaat tctttaccaa aaaaatcttt
     2641 ttcattatga actccgagcg attcttttat tttagcactg gcttcaggaa tgtaaggata
     2701 aacaaaatca gccacattag ccaaggttag ccacagcgag cttaacaatt cttctttttt
     2761 agctaaatct gttacggccc acacttgatt ttgattaata aacgcatcag cctgactaat
     2821 taattgtaaa aatgcctcgg cgccttgatt aaaacgaaaa tcctccaaag ccgcattata
     2881 tttttctttg cattcctcta tttcttttgc catcaaattt ttctcaattt tgatctttcc
     2941 accttgtttt tcaatcaaag ccactactcg atttaataaa ttgcccaaac cattcgctaa
     3001 ctctttatta tagcgttctt tcaaattatc aacagaaaaa tcgccatctt gtgataaaga
     3061 aaattctcgt aatagataat agcgcaaaga atcaacgcca aattctttaa tgagttcgcg
     3121 cgggtcaatc acattgcctt tactttttga catcttatct ccttctaaag taataaaacc
     3181 atgaatagca attttgttag gcaaacgaac acctgcagcc aaaagcattg ccggccaata
     3241 aatgcaatga aattttgcaa tatctttacc taagaaatgc cagacttccg ttgacggatc
     3301 ttcccaccaa gctcggtaca aaggcgaatt aactccataa cctaaaacag taatataatt
     3361 gcataacgca tcaaaccaaa cataaattac ttgcgaatta tcatttggca cagaaatgcc
     3421 ccaattatgc gcccgctcct gagagcgcga aatactaaaa tctttcagac cgctttctat
     3481 aaaagaaaca acttcttgtt tgcggttggg cggtatgatt tgaatcaaat cgttttgata
     3541 aatctcttct aattgttttt gaaaacgaga aagacgaaaa aaataatttt cttctttaat
     3601 cctttgcggt gctgtatgat gaattggaca taaaccatca actaattctt cttcttttaa
     3661 aaaatcctca caaccaacac aataaagtcc ttcgtattcg ctttgataaa tatctttttg
     3721 agcagctaac caaaattttt ttactccttc taaatgacgc ggctctgttg tgcgaataaa
     3781 atcagaaatc tcaatgttaa gtaatttcaa aaaatcttta aaaacctgcg ctttttcatc
     3841 aacaaattct ttaacattac gaccggcttt ttcggcagcc aaggcattct tcaagctgtt
     3901 ttcatcaact ccagtagcaa aaaaaacgtc gttaccctgt aagcgttcat aacgcgctgc
     3961 gcaatccgct tgaacaaatt ccaaagcatg accaagatgc ggattggcat ttacataagg
     4021 aatagaggtt gtaatataaa tttttttgag cataattaaa taatacttgt cgctttttta
     4081 attgcaaata atttctgtgc ttttaacacc tcatttttta actggcgccg caattgtaaa
     4141 aatcgttcct aataaaccat tagcttatat tttagcattt tttgctcatt tcattcttga
     4201 tcgcattcct cattccgatc acttcctcag cggcatcagc agcggccgta aaaataaatc
     4261 cttttaccag acaattggcc caattatttt tgattttttt attggaatta cccttattat
     4321 aattgcgtat tttacttttg acaacttcgc tttgactcca gttttaattg gtggattttt
     4381 gggcatttta cccgatgttt ttaatgctct ttcttatttt aactacaaga aaaattattt
     4441 acttctattc aaagggcaac ctctttatcc tgtcagtgaa tttattactc aaacaaaaaa
     4501 tccttattat cgttttcatc gcttttttca caatgaaaaa gtatacaatg ggatattagg
     4561 gacaattact caactcgctt ttgttgttgt tgatttaata gtattattgg ttttgtaaat
     4621 tattttaact aagacgggtg gcggcggccg ccaattctgg cggccgccgc cctcaaaaat
     4681 cgtttccaac tttaaggcag cggcgctacc tctaaccgct gctgtttctt tttattcaac
     4741 tctttaatta tttcttcaac tacaatcacc accggcaaag caatcaacat tcctaaaacg
     4801 ccgcctaatt taccacctgc aaacaaagcc accaaaacaa tcactggatt aaaaccaata
     4861 gttttgccca taattgctgg tactaaaata tcattttcta gacgctgaat aagaataaat
     4921 gcgatcgccg tccaaatcgc caaaggcagg gattgcgtta aggccacaat cgttgctacc
     4981 acgcctgcca aaattggccc caccattggc actaattcac aaa
//
LOCUS       DAOSMC010000028         4775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_19464_length_4775_cov_16.515042, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000028 DAOSMC010000000
VERSION     DAOSMC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19464_length_4775_cov_16.515042"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..455
                     /locus_tag="PL075_02540"
     CDS             261..455
                     /locus_tag="PL075_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD97085.1"
                     /translation="MVESSKIKLLREKLGLSQEKLARLADISNNTIIYIEAGKQHNPT
                     IETLEKIAKALNVPIEELIK"
     gene            460..1467
                     /locus_tag="PL075_02545"
     CDS             460..1467
                     /locus_tag="PL075_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97086.1"
                     /translation="MATGRTISASFLTDLDRKAIELCDSISKTISDSIPDEEAKKDFE
                     KEYGKISYTRDGGLGLGGGKIQRDAICTRGRQGKAPFSNRNLRWHPLIVAENKPGFAR
                     KIERIEIGGQDDGQILVFVVKDAKGKELKFPSDKVHEMPERFVALPEHWYPHINTLKH
                     WSDTLWTQNSCVIPALEACNWWDSVETYAILGLALAVDLYGADFDSLYTKIKQILEKQ
                     NINNKISLPTNIFPTKKEDITKCPVCLLNISKGLEAFRKSERGTTWQPAWRSSKKEEG
                     DDSSIQIMHVNPLVEMQIKHNSSNVRYGHRWCNVSMTDHSLDETLDFMEFVVRAHNRC
                     K"
     gene            1473..3482
                     /locus_tag="PL075_02550"
     CDS             1473..3482
                     /locus_tag="PL075_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOD97087.1"
                     /translation="METLYLFDLAEKTRVKKMIKIVDLKPINNLKLIFREIRDYFAGN
                     VTGITRDEKIAQNIMRLLFCKIYDEKNSRNIVEFSNRPDENYDLFQRRIDDLFEKVKN
                     NYPDIFEQNEKIEIKAEELSFIVSKLEDYSIINADRDVIGDAFEELIGTSFRGGEGQF
                     FTPRNVVQMMIDVLSPQEGERIIDPACGSGGFLAYTLRHFISNNKKNFYLTGIDKDAF
                     LAKIAKIYLSLLGDHEFHIYCENSLEVPIKWSKDTKENIQLGTFDVVLTNPPFGAKIP
                     VVGRELLSQYQLGHMWENGEKWLQTKKLLDKQSPQVLFIERCLQLLREGGRLGIVLPE
                     GIFGNPSDRYIWEYISSVASVIGIVSLSQETFQPSTHTKTSVLFLKKTTEKPKSIFMA
                     IAYNVGHNKNGKPVYKIDKTNSSKILDDDLPAIAKNFNEYNNKNKITDEHLGFRIAYS
                     DLNDHIFIPEYYNPEIRKELEVLKNSGKYSLISIGELIDKGILQIRRGNEIGSQFYGT
                     GEIPFVRTSDIVNWEIKFDPVKAVSDDIYNQYKKSQDIQEKDILFVNDGTFLIGRTAM
                     VTKLDLKIIIQSHLRKIRVLNTEFIDPYYLFYLLNSKIVRKQIDSKTFVQATISTIGN
                     RLSEIILPISNDQNEIQKITNETRDIINEKAELRKRTVKIVDESI"
     gene            3543..3893
                     /locus_tag="PL075_02555"
     CDS             3543..3893
                     /locus_tag="PL075_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97088.1"
                     /translation="MAGNLFFIRGWVITLITGLFVLFTKDTNQNYILIAYFLIIIFWV
                     LDGYFLSQERLFRALYDHVRVLDVKDIDFSMDTNEFKKNRKNCWLPSMFSQTLLIFYL
                     SLLCITTLIYSLFK"
     gene            3898..4401
                     /locus_tag="PL075_02560"
     CDS             3898..4401
                     /locus_tag="PL075_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOD97089.1"
                     /translation="MTTKRQVFYSFHYRPDCWLASQVRNIGVVEGNKPAPDNDWERII
                     SAGDDAIKRWINEQMKYRSCTIVLVGSKTANRKWINYEIIKSWNEGMGVVGIYIHGLK
                     NSEGYISDKGENPFDYIGYGDSGKKLSAIVKCYNPTGQNSKERYDWISQYIADAVEEA
                     IKIRNNN"
BASE COUNT         1681 a          733 c          924 g         1437 t
ORIGIN      
        1 gcgcttgaat ggcattcaag aggtcgccgg ttcgatcccg accagctcca catcatattg
       61 attagctgcc tcactcgggg cttcgcccct cgcttcggcg ggcctttctt tcttttcctt
      121 gccccaccct cccagcacag aaaagattaa ggaactccct ttgttcttta ctatttccaa
      181 atattttggt aatattatac tatggataaa aatatttagt atacaattct tataacacaa
      241 tttaatactg ctattataat atggtagaat caagtaaaat aaagctgctg agagaaaaac
      301 taggtttgtc gcaagaaaaa ctggctcgac ttgccgatat ttccaataat actattattt
      361 acatagaggc aggcaagcaa cataacccga cgattgagac gcttgaaaaa attgcgaagg
      421 cactaaatgt gccgattgag gaattaataa aataaacaca tggccacagg aagaacaata
      481 agtgcgagtt ttctaacaga tttagatcgt aaggctattg aattatgcga ttcaatatca
      541 aaaacgattt ccgattctat tcccgatgaa gaagccaaaa aagacttcga aaaggaatac
      601 ggtaaaatta gttatacaag agatggtggt ttgggattag gtggcggaaa aatacagaga
      661 gatgctatat gcacaagagg tcggcaaggc aaagcccctt tttcaaatag aaatttaaga
      721 tggcatcctt taattgttgc agaaaataaa ccaggttttg caagaaaaat agaaagaata
      781 gaaattggcg ggcaagacga cggtcagatt ttagttttcg tagttaaaga cgcgaaaggt
      841 aaggagctta aattcccctc agataaagta catgaaatgc cagaaagatt tgtcgccctt
      901 cctgaacatt ggtatccaca cattaacact ctaaaacatt ggtcagatac tttatggaca
      961 caaaattcgt gcgtgattcc tgcccttgaa gcgtgtaatt ggtgggactc ggttgaaacc
     1021 tatgctattt taggacttgc tttagctgtt gatttatacg gagctgattt tgattcgctt
     1081 tataccaaaa tcaagcaaat acttgaaaaa caaaatatta ataacaaaat ttcattaccg
     1141 acgaatattt tccctacgaa aaaagaggat attactaaat gtccagtatg tcttcttaat
     1201 atttcaaaag gtttagaggc atttagaaaa tctgagagag gtacaacatg gcaaccagct
     1261 tggcgttctt ccaaaaagga agaaggagac gattcaagca ttcaaataat gcatgtgaat
     1321 cctcttgttg aaatgcaaat taagcataat tcaagtaatg ttaggtatgg tcataggtgg
     1381 tgcaatgttt cgatgaccga ccactcgtta gatgaaactc tagacttcat ggaatttgta
     1441 gtgcgtgcac ataacagatg taaataattt ttatggaaac tttatattta tttgatttag
     1501 ccgaaaaaac aagagttaaa aaaatgatca agattgttga cttgaaacca ataaacaatc
     1561 ttaagttgat atttcgagaa attcgggact attttgctgg caatgtaact ggcattacaa
     1621 gggatgaaaa gatcgcacaa aatataatgc ggcttctttt ttgtaaaatt tatgatgaaa
     1681 agaatagcag aaacatagtt gaattttcta accgccccga tgaaaattat gatttgtttc
     1741 aaagaagaat tgatgattta tttgagaaag tcaaaaataa ttatcctgat atttttgagc
     1801 aaaatgaaaa aatagaaata aaagctgaag aactttcttt tatagtttca aaattagaag
     1861 attattcaat cataaatgca gacagagatg ttattggtga tgcctttgaa gagttgatag
     1921 gcacttcttt tagaggtgga gaagggcaat ttttcacacc aagaaatgtt gtgcaaatga
     1981 tgattgatgt tttgtctcct caagaagggg agcggattat tgaccctgcc tgtggtagtg
     2041 ggggatttct cgcctataca ttaagacatt ttatttccaa taataagaaa aatttttatc
     2101 ttactggaat tgataaagat gctttcttgg caaaaatagc aaaaatttac ctttcattac
     2161 ttggcgacca tgaatttcat atttattgcg aaaacagctt agaggttccg attaaatgga
     2221 gcaaagatac caaagaaaat atccagcttg gcacttttga tgttgttctt acaaatcctc
     2281 cttttggggc aaaaattcct gttgttggga gggaattatt gagtcaatat caactaggcc
     2341 acatgtggga aaatggcgaa aaatggctac aaacaaaaaa attacttgat aagcaatccc
     2401 cccaagtctt atttattgaa agatgcctcc aattgctccg cgagggcggt cgccttggca
     2461 ttgttttacc agaaggtatt tttggtaatc cgtctgatag atatatctgg gaatatatct
     2521 caagcgttgc ctcagttatt ggaattgtaa gtttatctca agaaactttt caaccaagca
     2581 cacatactaa aactagtgtt ttatttttga aaaaaactac cgagaagcca aaatcaattt
     2641 ttatggcaat tgcctataat gttggtcata ataaaaacgg aaagcccgtc tataaaatag
     2701 ataaaaccaa cagtagtaaa atcttagatg atgaccttcc tgctattgca aagaatttta
     2761 atgaatataa caacaaaaat aaaattacag atgaacattt aggatttcgt atcgcttatt
     2821 cagacttaaa cgatcatatt tttattcctg agtactataa tcctgaaatc cgcaaggagt
     2881 tagaggtatt aaaaaatagt ggcaaatatt cgttaatctc aattggagag ttaatagaca
     2941 aagggatttt gcaaattaga agaggtaacg aaataggttc gcaattctat ggaacaggag
     3001 agataccttt tgtaagaact agtgacattg ttaattggga aattaaattt gatccagtaa
     3061 aagccgtttc tgatgatata tacaatcaat acaagaaatc tcaagatatt caagagaaag
     3121 acattttatt cgtaaatgac ggaacatttt tgattggacg tactgccatg gttacaaaat
     3181 tagatttaaa gataattatt caaagtcatt taagaaaaat cagggtacta aatacagaat
     3241 ttattgatcc gtattatttg ttttatctct tgaatagtaa aattgtaaga aagcaaatag
     3301 attctaaaac atttgtacag gcaacaattt caactattgg aaatagattg agtgaaatta
     3361 ttctgcctat aagtaatgat caaaatgaga ttcaaaaaat tactaatgag accagagata
     3421 ttattaacga gaaagctgaa ttaaggaaaa gaacagtaaa gatcgttgat gagagtattt
     3481 aataattaaa aatggaaaat aagcaacaac atttagagtt tattcagggt gcaattaacc
     3541 gaatggcggg aaatttattt ttcattagag gttgggtgat tactttgata actggacttt
     3601 ttgtgttgtt taccaaagat acaaaccaaa attatatttt gattgcctat tttcttataa
     3661 taatattttg ggttttagat ggatattttc tttcgcaaga gagattattt agggctttat
     3721 acgaccacgt tagggtgctc gatgtgaaag acattgattt ttctatggat acgaatgaat
     3781 ttaagaaaaa tagaaaaaat tgctggttgc catcaatgtt ttcccagacg cttttgattt
     3841 tttatttatc actactctgc attacaactt taatttattc cttgtttaag taaagatatg
     3901 acaacaaaaa gacaagtatt ttatagtttt cattacagac ccgattgttg gctggcatcc
     3961 caggttcgca atataggagt tgtagaagga aataaacccg cccccgataa tgattgggaa
     4021 cgaattataa gtgctggtga tgacgccatt aagaggtgga taaatgaaca aatgaaatat
     4081 aggtcatgca ctattgtgct tgttggaagt aaaaccgcaa acagaaaatg gataaattac
     4141 gaaataataa agtcgtggaa tgaaggtatg ggcgtcgttg gcatttatat tcatgggcta
     4201 aaaaatagtg aagggtatat ttccgataaa ggcgaaaatc catttgatta tataggttat
     4261 ggcgatagtg gtaaaaaatt atctgcaata gttaaatgtt ataatcctac cggacaaaat
     4321 agtaaagaga gatacgattg gatttcacaa tacatcgctg atgcggttga agaagcgata
     4381 aagattagga acaataatta agcccgccga aaaaaccgcc gtgggcttct agtggacagg
     4441 aaaaaaaggg gtctgaatgt tattcccacc aatgaatttt tgcccgctcg cctcgtccgg
     4501 gggcgggaaa gcgtttctgt aattctgcgt tagcagaata tcaaaacaga aaaattttct
     4561 tttccttaat tgaaaaaaat ttgggaggcg cgcaactaaa taatgttaat aaaatttttc
     4621 tgttttgctc gccgtcaagc gagcggaaac gctgggcggg tcattcagca aaatcctctg
     4681 gattttgctc aaagaaagtt ctgacatcgt tcagcaaaca ccacatcacc aaaacgtaga
     4741 acaggctttt tagcctgttt ttaattatct ataaa
//
LOCUS       DAOSMC010000029         4538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_20727_length_4538_cov_16.118447, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000029 DAOSMC010000000
VERSION     DAOSMC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20727_length_4538_cov_16.118447"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..374
                     /locus_tag="PL075_02565"
     CDS             165..374
                     /locus_tag="PL075_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOD97090.1"
                     /translation="MSREISPIGKNIKKLRKQKGLSQDRLSKLADISYNTVIKLESGG
                     ITNPSIDTLQKLAKALDVGVDDLLK"
     gene            380..1063
                     /gene="radC"
                     /locus_tag="PL075_02570"
     CDS             380..1063
                     /gene="radC"
                     /locus_tag="PL075_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOD97091.1"
                     /translation="MDKSFTIHDLPKDERPRERLVKFGEQALSAQELLQLILGRGVAG
                     ESVAVTAQKLLSHFGNLEKLSEASIEELSSIKGIGLAKAAQIKAVFEISRRLSTQAPT
                     YKGKKLTDPEKVYRLIKSKLKDYHKEHFYIIALNSRNHSIAEVSVGSLNESVVHPREV
                     FAEAIKNKAASVVFAHNHPSGDPEPSENDLVLTKKLVESGKILGIEVADHIIITKDNF
                     FSFKNKGII"
     gene            1068..2510
                     /locus_tag="PL075_02575"
     CDS             1068..2510
                     /locus_tag="PL075_02575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD97092.1"
                     /translation="MAKDNKKLEVEKLKTEIERLKKELKKRKKYGLVWEEKPEELVEM
                     CKEKLPVLKEVKNKEIITDKDKPVNLLIEGDNYHALSVLNYTHAKKVDVIYIDPPYNT
                     GQKDFKYNDQWVDIEDTYRHSKWLAFMEKRLVLAKNLLKNNGVIFIQIDDNEIAQLKL
                     LCDEIFGPNNFRNMLVWAYRTGGSPSKKDVFGRKHDYILFYSRSDNYFFNVLKEKIYY
                     QKDFFGAKKDEKGNFYAEVNLRDVVEGEIELFKGGKFYKKINVKPVLNLSSERVMDFP
                     SQKPIGLIKFLLEVTTNKDSIICDFFAGTGSTGEAVLDLNRDDNGNRKFILCTNNENN
                     IATDICYPRIKKAIFGFKNRPALGGNLKYFKTDFVDYDEPTDKNKIKLTRQATEMLCV
                     REGTFERVEDNEKFKIFKNSDHYTGIIWDQTAIPAFKKAIKDIEGKFSVYVFSLGDEA
                     FDDEFKDVKQKVQLSPIPEAILRVYRRIFR"
     gene            2516..4192
                     /locus_tag="PL075_02580"
     CDS             2516..4192
                     /locus_tag="PL075_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD97093.1"
                     /translation="MKIKSLKIHNFRSIKEQSFDLTDYSLLVGANDSGKSNTIDAIRV
                     FYDDLKFSEEKDWPKFTTDDDECWIEIKFILSDEEFDNLKDEYKQNDDGKKCFTVRKY
                     LKHQSKVQSGSSNIYGYENGQLSDNFFYGWKNVAKGKLGDILYIPAVSQVGDYTKLSG
                     PSYLRNILDFICKKIISSGKSFNNLSKTFDEFTKNFKQEKTKEGLSIEGFEQDINEAI
                     SDRKLKIDLSIRSLNVADITKNLIEPYIKDEILGDHKLPIESFGEGLQRRLVYTLIKL
                     STKYKEKKTTKDKKEFFSNFLLILFEEPEAFLHSSQQEILNQSLKDLSSDKNQQVLIS
                     THSVHFVSKNIDSLPSILKLYKQDGKTQVYQITEPELKQILIANKDLKRILGETIEDK
                     DLDLEAIRYSLWLDPDRCCAFFADFVLICEGASEKILIDYLIKNKEIEPSNKEIYILN
                     AMGKENIHRYMNLFKELGIKYSILFDGDKNSSNQNRHQKINEFLQNNKNEYTKTIDYF
                     EHNLECFLGIPEENRGDKKPLNVMWHYENNKIDSKKLGDFKGKIEKLLAG"
     gene            4198..>4538
                     /locus_tag="PL075_02585"
     CDS             4198..>4538
                     /locus_tag="PL075_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOD97094.1"
                     /translation="MQLKIYQENAIDDLLTKAKRLLGYSGSKKLVFKSPTGSGKTIMM
                     AEFFKQLVDDREVKQSLGFIWTAPRQLHIQSRDKLESYFETSRALKCSYFEDLDDRKI
                     SENEILFFNWE"
BASE COUNT         1701 a          654 c          845 g         1338 t
ORIGIN      
        1 tccttccccc aagccccctt cctttttgtc cgcccgccat gcccgcttcg cgggcatttg
       61 gaattaaact ccctaaaatt ccctttacat tatcaaaata attttgtatc ataattatag
      121 ataaggatta ctctatacat acattctatt attttgatac tactatgtca agagagattt
      181 cgccgatagg caaaaatata aaaaagttaa gaaaacaaaa gggcttatcg caagaccggc
      241 tttctaaatt ggctgatatt tcctataaca cggtgataaa gttagagtcg ggcggtatca
      301 ccaacccttc aatagataca cttcaaaaat tagccaaagc ccttgatgtt ggcgttgatg
      361 atttattgaa gtaaaaatta tggacaaatc atttactatc cacgacttgc caaaagatga
      421 gcgtccgcga gagcggttag ttaagtttgg cgaacaggca ctttcagctc aagaattatt
      481 acaacttatt ttagggcgag gcgtcgccgg tgaatcagtg gcggttacag ctcaaaaatt
      541 attaagtcat tttggaaatt tagaaaaatt gtcagaagca agtattgaag aactatcctc
      601 aattaagggg attggtttgg caaaggccgc tcaaataaaa gccgtttttg aaattagccg
      661 tcggctttca actcaagccc cgacttacaa aggcaaaaaa cttaccgacc ccgaaaaagt
      721 ttatcggctg ataaaaagca aacttaaaga ttatcacaaa gaacattttt atattatcgc
      781 tcttaatagc agaaaccact caattgcaga agtttcagtc ggtagcctca atgagagcgt
      841 tgttcacccg cgcgaagtat ttgcggaagc cattaaaaac aaagccgcct cggttgtatt
      901 tgcacataat cacccttcgg gcgaccccga accttcagaa aacgatttag ttttaactaa
      961 aaaattagta gagtccggca aaattttagg catagaagtc gctgaccata ttattataac
     1021 taaagacaat ttctttagtt tcaaaaacaa aggaataatc taaaactatg gcaaaagata
     1081 ataaaaaatt agaagttgaa aaattgaaaa ccgaaattga gcggctaaaa aaagaattga
     1141 agaaacggaa aaaatatggg cttgtttggg aagaaaagcc cgaagaattg gttgagatgt
     1201 gtaaggaaaa actgccggtg ctgaaagagg ttaaaaacaa agaaataatc accgacaaag
     1261 ataagccggt aaatttattg attgaaggcg acaattatca cgctctttct gttttgaatt
     1321 acactcacgc aaaaaaagtt gatgtcattt atattgatcc gccatataac accgggcaaa
     1381 aagacttcaa gtataacgat caatgggtgg atatagaaga tacttatcgc catagcaagt
     1441 ggttagcatt tatggaaaaa cgcttagtat tggcaaaaaa tttattaaag aataatgggg
     1501 ttattttcat acaaattgat gataatgaaa tcgctcaatt aaaattacta tgcgatgaaa
     1561 tctttggccc aaataatttt agaaatatgc ttgtttgggc ttatagaacc gggggctcgc
     1621 caagtaaaaa agatgttttt ggtagaaaac acgattatat tcttttttac tcaaggtctg
     1681 ataactattt tttcaatgtc ttaaaagaga aaatttatta tcaaaaagat ttcttcggtg
     1741 ccaagaaaga cgagaagggt aatttttacg cagaagtaaa tttaagagat gttgttgaag
     1801 gagaaataga actatttaag ggtggtaaat tttataaaaa gataaatgta aaaccagttt
     1861 tgaatttatc aagtgaaagg gtgatggatt ttccttcaca gaaacccatc ggacttatta
     1921 aatttcttct agaagttaca actaacaaag atagtataat ttgtgatttt tttgccggaa
     1981 ccggcagcac tggcgaagcg gttttagatt taaatagaga tgataacggt aataggaaat
     2041 ttattttatg tacaaacaac gaaaacaaca tcgctactga tatttgttat ccgagaatta
     2101 aaaaggcaat ttttggattt aaaaatagac cagcattagg cgggaattta aagtatttca
     2161 aaaccgattt cgttgattat gatgagccga cagataaaaa caaaattaaa cttacaagac
     2221 aggcaactga aatgctttgt gttagagagg gaacttttga aagggtggaa gataatgaaa
     2281 aatttaaaat tttcaaaaat tcagaccact ataccggcat tatttgggac caaacagcaa
     2341 ttccggcttt caaaaaggca atcaaggata ttgaaggaaa attcagcgtc tatgttttct
     2401 ctcttggtga tgaagctttt gatgacgaat tcaaagatgt aaaacaaaaa gtccaattat
     2461 cacctatccc cgaagcaatt ttaagggtgt ataggaggat ttttaggtag agattatgaa
     2521 gataaaatct ttaaaaatac acaattttcg tagtattaag gagcagtctt ttgatttaac
     2581 tgattattca ttgttagttg gtgctaatga ttcaggaaaa tcaaatacta tagacgccat
     2641 cagagttttt tatgatgatt taaaatttag cgaagaaaaa gattggccta aattcactac
     2701 agatgatgac gaatgctgga ttgaaattaa atttatactc tctgatgaag aatttgataa
     2761 tttaaaagat gaatacaaac aaaatgatga tgggaagaag tgctttactg taaggaaata
     2821 tttaaaacat caaagtaaag ttcaaagtgg atcaagtaat atttacgggt atgaaaatgg
     2881 tcaactttca gataattttt tttatggatg gaagaatgtg gcaaaaggta aactgggtga
     2941 tattttatat atacctgcag taagtcaagt cggtgattat acaaaactta gtggaccttc
     3001 atatctacgc aatattctag attttatatg caaaaagatt attagcagtg gcaaatcttt
     3061 taataacttg agtaaaactt ttgatgaatt caccaaaaat tttaagcagg agaaaacaaa
     3121 ggaagggttg tctatagaag gatttgaaca agatataaat gaggctattt cagatagaaa
     3181 gttaaaaata gatttaagta ttcgctctct taatgtagcc gatattacaa aaaatttaat
     3241 tgagccctac attaaagacg aaattttagg agaccataaa ttaccgatag aatcatttgg
     3301 agaagggctt caaagacgat tggtgtatac attgataaaa ttatcaacta aatataagga
     3361 gaagaaaaca acaaaggata aaaaagaatt tttttctaat tttttgttaa ttctatttga
     3421 ggaacccgag gcattcttgc attcttccca acaagaaatt ctaaatcaaa gcctaaaaga
     3481 tctctcaagt gataaaaatc aacaggtttt aatttctacc cattctgtcc attttgtaag
     3541 taaaaatatt gatagcttgc cttcgatttt aaaactctat aaacaagacg ggaaaacaca
     3601 agtctatcag attactgaac cagaattaaa acagatttta atagccaata aagacttaaa
     3661 aagaatttta ggagaaacta tcgaagataa agatttagac ttagaagcga taaggtattc
     3721 cttatggttg gatcctgata gatgctgtgc attttttgct gattttgttt taatctgtga
     3781 aggtgcttct gaaaaaatct taatagacta ccttataaaa aataaggaaa ttgagccatc
     3841 aaacaaagag atttatattt taaatgctat gggtaaggaa aacatccatc gttatatgaa
     3901 tttatttaaa gaactgggga taaaatattc tatattattc gatggcgata aaaatagtag
     3961 caatcaaaac cgtcatcaaa aaataaatga gtttttgcaa aataataaaa atgaatatac
     4021 aaaaacaatt gattattttg aacataattt agaatgtttt ttaggtattc ccgaggaaaa
     4081 ccgaggcgat aaaaaacctc tcaatgttat gtggcattat gagaacaaca aaattgacag
     4141 caagaaactc ggtgatttta aagggaaaat tgaaaagtta ttagcaggat aaaatatatg
     4201 caattaaaaa tttaccaaga aaacgcaatt gatgatttac tgaccaaggc caagcggctt
     4261 ttggggtatt ctggcagtaa aaaattggtt tttaaatcgc cgactggttc ggggaaaact
     4321 attatgatgg ctgaattttt taaacagttg gttgatgata gagaggtaaa acaatcgctt
     4381 ggttttatct ggactgcccc aagacagctt catattcaaa gccgagataa attagaaagc
     4441 tattttgaaa caagccgggc gttaaagtgt tcctattttg aagatttgga cgatagaaaa
     4501 ataagcgaga atgaaatttt attttttaac tgggaaag
//
LOCUS       DAOSMC010000030         3931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_24982_length_3931_cov_19.264448, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000030 DAOSMC010000000
VERSION     DAOSMC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24982_length_3931_cov_19.264448"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1898)
                     /locus_tag="PL075_02590"
     CDS             complement(<1..1898)
                     /locus_tag="PL075_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97095.1"
                     /translation="MKLQLLKAKIKKILTHPKIISGILIGVILNFLLTYKASAFDLFG
                     IGSLAERAVYQAVIWIGNVIASIAGIFINLESQLLSLITQVSQFTTMPVVQTGWQVAR
                     DFANIFFIALLVYVAFCYILRINSKSSQKILVNVIVMAILINFSLMIGGIIIDFSNVF
                     FNYFVFGTTANQQSSEDKSAALSYSLGNALKLNTFTKTLQEAQKQEEVAEEELFEKAK
                     SSTSTEDMQNWVTFKMDNEDTHVLFFAAIRIIFIIIFSFVIILALGGLVVTLFVRLFW
                     LWILLILAPLAWTLSVAPIPVIGHYAKEWWNNFLKWCFVGPVVGFFIFLALTLANKMP
                     YIPQEALTAFSGTFFSGASDTLFSVQVVMQLALIVGLIIAGLIAGQKMGDVGAKFALG
                     LATKTGKATGNAIKNRASLAGLRAARIVAEPVAEGLAKAPKGLKALGAVTGIGALSRA
                     TISAAEKRDQAEEQKKMKKYEALDKSELEKIAASPIREREKTAAIAALVKKTGGDIDI
                     KFRDDAMNLLPKFHMHGEYRDVIKAHPEWDKRLTEAAKKGAAELEKATAEVFKDKDIS
                     AIIIPKLPDPGSPYYEELKMIRNSILRYKAQLGDPKDMARSLVAANKRNAAQLCEDMN
                     QVITEFYK"
     gene            complement(1907..3469)
                     /locus_tag="PL075_02595"
     CDS             complement(1907..3469)
                     /locus_tag="PL075_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97096.1"
                     /translation="MKTKIVIIFLIVSLIGVELLPVFVDKASAQEVPLLPLPSPSSLK
                     LPDMSQMEPVIANDSEAFNLSWDEVLLCSSGLSGTIFSLLGELKQIILNPVCQALGRE
                     ASFLGIGGTVISGMCTINVGGSDQIQFCKDWLQKKKATIKARAAETAKILLGYAAKTF
                     MDHLVYSTVDWISGRTTGEPQFVTNWQAFFNDVADEAFGRFIENSPFSELCEPFRFQV
                     RATTRLPGRPPFPTCTLTQVLSNLEYFYSDFSNGGWLVFEQSFYPWNTADGAWLMTQE
                     ALEYELAQAQMEAEKKTQTGYQPIEDCVHKIKNYITGDTECWDSIISAPSQTVSDVAS
                     KAVTAQIDKTESYFLTSADLSNYATMIGQALITRLIKSAKEELIGGKWYGKGLLDLPE
                     KGDEGPSLNLRYSCKNYDGMGSCEYDANGSYTSLESCQAACSKNQYRWGCNTETGICE
                     YQASGPYTDFTTCSNACSESTSTPTTTSMFWTCDSSTGECVEDENGDFRSLQGCLKMC
                     NYKEPGQEVPSY"
     gene            complement(3471..>3931)
                     /locus_tag="PL075_02600"
     CDS             complement(3471..>3931)
                     /locus_tag="PL075_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97097.1"
                     /translation="DFWNNLKLVQIDELKFGEENNPITSLQYFVNVMQVINDNHLTDD
                     IYLENLQSFIDNEDPAYLKDFISKLDNGIIALKNIPIPPSYAALHLDLINFMNLKRTV
                     LVDLYNYTSDPIRALVASDFLTEIDDYYQNWVNTLTNKMEQDGSLNYFQF"
BASE COUNT         1256 a          824 c          645 g         1206 t
ORIGIN      
        1 tctttataaa attcggtgat gacctgattc atatcttcac acaattgtgc agcattccgt
       61 ttatttgctg ccacaagaga tcgagccata tcctttggat cgcctaattg agctttataa
      121 cgcaaaatgc tattccggat catctttagt tcttcataat aaggacttcc gggatcgggt
      181 aatttaggaa taattattgc agagatgtct ttgtctttaa acacttcggc tgttgccttt
      241 tctaattcag ccgcgccttt cttagctgcc tctgttaatc gtttatccca ttctggatga
      301 gcttttatta catcacgata ttctccatgc atatggaact tcggtaataa attcattgca
      361 tcatctctaa atttaatatc aatatctccg ccagtttttt tgactaaagc ggcaatagca
      421 gcagtttttt ctcgctctct aataggcgaa gcagcaattt tttctagttc tgatttatca
      481 agcgcttcat atttcttcat cttcttttgc tcctctgcct gatctcgctt ttcagctgct
      541 gaaatcgtgg cacgactcaa agcgccaatt cctgtcaccg ctcctaaggc ctttagtcct
      601 ttgggcgctt tggccaaacc ttcagctact ggttcagcta ctatacgagc tgctcttaat
      661 cctgctaaag aagctctatt cttaatagca ttgccagtag ccttgcctgt tttagtagct
      721 aaacccaaag caaattttgc accaacatcg cccatttttt gaccggctat taaaccagca
      781 ataatcaaac caacaatcaa agccaattgc ataaccactt gaacactaaa caaggtatca
      841 ctggcgccag aaaagaatgt accgctaaaa gccgtaaggg cctcctgtgg tatataaggc
      901 atcttgttag ctaaagtcaa agctaaaaat atgaaaaaac ctacaacggg tccaacaaaa
      961 caccatttca aaaaattatt ccaccattct ttagcataat gaccaatcac cgggatagga
     1021 gcaaccgaaa gagtccaagc cagaggcgct aaaataagta gaatccaaag ccagaaaaga
     1081 cggacaaaaa gcgtcacaac taaaccaccc aaagcaagaa taatgacaaa actaaaaata
     1141 ataataaata ttattctaat agcagcaaaa aacaaaacgt gggtgtcttc gttatccatt
     1201 ttaaatgtta cccaattttg catgtcttct gtcgaagtac tcgacttcgc cttctcgaac
     1261 aattcttctt ctgctacttc ctcttgtttt tgagcttcct gtaatgtttt tgtaaaagta
     1321 tttaatttta aagcattgcc caaagaatag cttaaagctg cgcttttatc ctctgagctt
     1381 tgctgattgg cagtagtgcc aaacacaaaa taattaaaaa agacgttgga gaaatcaata
     1441 ataatgcctc caatcattag gctaaaatta atcaaaatcg ccattacgat aacattaact
     1501 aaaatctttt gactgctttt gctattaatt cttaaaatat aacaaaaagc tacataaaca
     1561 agcagggcga taaaaaaaat attggcgaaa tcgcgagcaa cttgccaacc cgtttgcact
     1621 actggcatag tcgtaaactg cgaaacctga gtaatcaaag acagcagttg tgactctaaa
     1681 ttaataaaaa tacctgcaat agaggcaata acattgccta tccaaatcac agcttgatag
     1741 accgctcttt cggcaaggct accaatccca aaaagatcaa aggcgctggc tttgtaagta
     1801 agcagaaaat tcaaaataac tccaattaaa attccggaga ttattttagg atgggtgaga
     1861 atttttttta ttttcgcttt aagtagttgg agtttcataa ttaagattaa taactcggaa
     1921 cttcttggcc cggctcttta taattgcaca tcttgagaca tccttgaagg ctacgaaaat
     1981 ctccattttc atcttcaaca cattcaccag ttgaagaatc gcatgtccaa aacattgaag
     2041 tagttgtcgg cgttgaagtg ctctccgaac acgcattgga acaagtagta aaatctgtgt
     2101 agggaccact agcttgatat tcacaaatcc ctgtttccgt attgcatccc caacgatatt
     2161 gatttttact acaagctgct tggcaacttt ctaaagaagt ataggaaccg ttagcatcat
     2221 attcacagct acccatacca tcataatttt tgcaactata ccgtaaatta agagaaggac
     2281 cttcgtcacc cttttccggc aaatccaata atcctttgcc ataccattta ccaccaatca
     2341 attcttcttt agctgattta attaaacgag taattaaagc ttggcctatc atcgtggcat
     2401 aattggataa atctgctgag gttaaaaagt aactttcggt tttatctatt tgagctgtca
     2461 ctgctttgga agccacatca gaaacagttt gtgaaggagc gcttattatt gaatcccaac
     2521 attcagtgtc tcctgtaata taatttttta tcttatgaac acaatcttct attggttgat
     2581 agccagtctg tgtttttttc tcggcttcca tttgagcttg agctaattca tattcaagcg
     2641 cttcttgagt cattagccaa gcgccatcag ctgtattcca aggataaaat gattgttcaa
     2701 aaaccaacca accaccatta ctaaaatcac tataaaaata ttctaagttg cttaaaacct
     2761 gcgttagagt acaggttggg aaaggcggtc gtcctggcaa tcgagtagtt gcgcgtactt
     2821 gaaaacgaaa tggttcacat aattctgaaa aaggagaatt ttcgataaac cggccaaaag
     2881 cttcgtcggc tacgtcgtta aagaaagcct gccaattagt cacaaattgt ggctcacctg
     2941 ttgttcggcc gctaatccaa tcaaccgtac tataaactaa atgatccata aaagttttag
     3001 ccgcataacc taaaagtatt ttggctgttt ctgctgctct agctttaata gttgccttct
     3061 ttttttgaag ccaatcttta caaaattgga tctgatcact tccgccaaca ttaatggtac
     3121 acattcctga aataacggtt cctccaatac caagaaacga agcttcacgc cctagcgctt
     3181 gacaaaccgg atttaaaatt atttgtttta attcgccgag cagtgagaaa atcgtcccag
     3241 aaagtccgga tgaacacagc aatacctcat cccagctaag attaaaagct tctgaatcat
     3301 tggctataac tggctccatt tgcgacatat ctggcagttt caaactggat ggtgatggta
     3361 atggtagtaa tggtacttct tgagcgctag ctttgtcaac aaaaactggc aataattcta
     3421 cgccgattaa agaaacaata agaaaaatga tgacgatttt tgttttcata ttagaactgg
     3481 aaataattta aactcccgtc ttgttccatt ttattagtta aagtatttac ccagttctga
     3541 tagtaatcat ctatttctgt taaaaaatcg ctagctacca aagcccgaat cggatcgcta
     3601 gtataattat aaaggtcaac caaaacagtt ctctttaggt tcataaaatt aattaaatcc
     3661 aaatgaaggg cagcataaga aggaggtatg gggatgtttt ttaatgcaat aattccatta
     3721 tccagcttgc taataaaatc tttcaaataa gctgggtctt cattgtcaat aaagctttgc
     3781 aaattttcca aataaatatc atctgttaaa tgattatcat taataacttg catgacattc
     3841 acaaaatact gcaaggaagt aattggattg ttttcctctc caaattttaa ttcatcaatt
     3901 tgaactaatt ttagattatt ccaaaaatct a
//
LOCUS       DAOSMC010000031         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_26921_length_3717_cov_20.631895, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000031 DAOSMC010000000
VERSION     DAOSMC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26921_length_3717_cov_20.631895"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1875)
                     /locus_tag="PL075_02605"
     CDS             complement(<1..1875)
                     /locus_tag="PL075_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOD97098.1"
                     /translation="MKLKSLEILGFKSFANKIVFSFPANFTAIVGPNGSGKSNIVDAI
                     RWVLGERDLKKLRVARADEVIFNGTPAKPRMSLAQVSMTLDNSQNELPIDFKEVVITR
                     RLERGKENQYFINDAAVRLKDVVELLAKAKIGSEGLTIINQGLSDALLKASPKERRVM
                     IEESLGLREFQLKKEDAKRKLEASQINLEKTQALLEELKPHLRSLRRQIHRWEKREEI
                     TQTLSILEHAFYAAKLKEIYLEKQQIDNERQKIEAELKQQEATLKELERQFDELEKKR
                     PDFSQQFQDLRRKIDEIEKQKGDLLKSLGRIEGQIEGLKRFAPVNVNEPTQSELVQAL
                     KQIKEELNQVLNFDLEKAREKIKQLIKEIEKLFTQENISTNEKEIATLQNEKDKILAQ
                     IEKFNQQVKNYNEELDTLRKKDEEVGNDSHGVLLELQKQRNILTDYNRQLNEISLRME
                     KNKLHEADLIMRLKESELNPVEFQWEKIKDFDTEKILKENNISGLDDLEMRILRFRRE
                     LANIGEIDELTLKEAKETEDRYQFLSQQSEDLIKAKADLEKIIAELTERIDTQFNESL
                     KIINDEFNKYFRMIFGGGRAKLSVIKMPKRQRKEKNEENNEEEKNKLREVEAIKVEEE
                     Q"
     gene            complement(1879..2616)
                     /gene="rnc"
                     /locus_tag="PL075_02610"
     CDS             complement(1879..2616)
                     /gene="rnc"
                     /locus_tag="PL075_02610"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOD97099.1"
                     /translation="MDKFEELEENIKIKFKNIDLLQQAFSHRSYLNENPRWRLGHNER
                     LEFLGDAVLELVVSEYLFKKFPNEPEGFLTSLRASLVNAETLADAANNLNFNKYLLLS
                     RGEAKDLGLGRRYILANTFEALLGAIYLDSGYKKCQQFVEENLINVYLEEILKEGRYR
                     DAKSLLQEKAQAEINVTPVYKTLNEWGPDHAKHFQVGVYLGDKLISEGEGTSKRLAEQ
                     EAAKKALETWQSLEEDLEKQKKDQIDK"
     gene            complement(2653..3216)
                     /gene="nusB"
                     /locus_tag="PL075_02615"
     CDS             complement(2653..3216)
                     /gene="nusB"
                     /locus_tag="PL075_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOD97100.1"
                     /translation="MATRHLCRSILLQSLYEWDFNRANLKRDIDLMATIDRNMKEALS
                     FIDEPDFVYRLAKNLVDHFTEIDNIIQKTAPEWPLDQINLIDRNILRLGISELIFGPK
                     DEVPPKVAINEAVELAKTFGSESSSKFVNGVLGTIYRQLVEEGVIKESVPKSKKEVSQ
                     NSAEEEENETTMQNEKNIEGDSLLEQK"
     gene            complement(3220..3450)
                     /gene="rpmF"
                     /locus_tag="PL075_02620"
     CDS             complement(3220..3450)
                     /gene="rpmF"
                     /locus_tag="PL075_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOD97101.1"
                     /translation="MGGVPTKHSTHGKVRRRRSHLKIQSGGFIACPKCGAAIIPHRYC
                     PNCGYYAQKEVVDVLAKLSKKEKKSRAKEIKK"
BASE COUNT         1026 a          771 c          527 g         1393 t
ORIGIN      
        1 ctgctcttcc tccaccttga ttgcctctac ttcccttaat ttattttttt cttcttcatt
       61 attctcttca tttttttctt ttcgctggcg cttgggcatt ttaatcaccg ataattttgc
      121 ccgaccgcca ccaaaaatca ttcggaaata tttattaaat tcatcgttaa taatttttag
      181 cgattcatta aattgtgtat ctattcgttc tgtcaactcc gcaataattt tttctaaatc
      241 cgctttggct ttaattagat cctcggattg ttgagataaa aattggtaac gatcttctgt
      301 ctcttttgcc tcttttaaag ttagctcgtc aatttcacca atattggcca attcgcgccg
      361 aaagcgcaaa atcctcattt ctaaatcatc taatccggaa atgttatttt cttttaaaat
      421 tttttcggta tcaaaatctt taattttttc ccattgaaat tcaactggat ttaactcact
      481 ctcttttaaa cgcataatta aatctgcttc atgcaattta ttcttttcca ttcttaaaga
      541 aatttcattg agctggcgat tgtaatctgt taaaatgttg cgctgttttt gcaattctaa
      601 aagcacgcca tgagaatcat tgcccacctc ttcatctttt tttcttaaag tgtccagttc
      661 ttcgttgtaa ttttttactt gttgattaaa tttttcgatc tgagctaaaa ttttatcctt
      721 ctcattttgt aatgtcgcaa tttctttttc attagtagat atgttttctt gagtaaataa
      781 tttttcgatt tctttaatta attgcttgat tttctctcgt gctttttcca gatcaaaatt
      841 taaaacttga tttaattctt ctttgatttg tttaagcgct tggaccaact ctgattgagt
      901 gggctcatta acatttactg gcgcaaagcg ttttaatcct tcgatttgcc cttcaattcg
      961 gcctaaactc tttaataaat cccctttttg tttttctatt tcatcaattt ttctccttaa
     1021 atcttgaaat tgctgagaaa aatctggccg ctttttttct aattcatcga attgccgttc
     1081 taattcttta agagtcgctt cttgctgttt tagctctgct tcaatttttt gacgttcatt
     1141 atcaatttgc tgtttttcca aataaatttc ctttaatttc gcggcgtaaa aagcgtgttc
     1201 taaaatagaa agtgtttgag tgatctcttc gcgtttttcc caacgatgaa tttgtcgccg
     1261 caaggaacgt aaatgaggtt ttaattcttc aagcaacgct tgcgtttttt ctaaattaat
     1321 ctgactcgct tccagttttc ttttagcatc ttcttttttt aattggaatt ctcgcaagcc
     1381 caagctctct tcaatcatta ctcgcctttc tttaggagaa gctttcaaaa gagcatcact
     1441 caatccctga ttaataattg ttaaaccctc acttccaatt ttcgcctttg ccaacaactc
     1501 caccacatct ttcaaacgta cggcagcatc attaataaaa tattgatttt ctttaccgcg
     1561 ttccaaccgc cttgtaatta ccacttcctt aaaatcaata ggtaattcat tctgactatt
     1621 atctaaagtc attgaaactt gagccagcga cattcgtggt ttagctggtg tgccattaaa
     1681 aattacttcg tcggcacgcg ccactcgtaa tttttttaaa tctctctctc ccaaaaccca
     1741 acgaatggca tcaacaatat ttgatttacc gctgccattc ggcccaacaa tagctgtaaa
     1801 attagccgga aaagaaaata caattttatt agcaaatgat ttaaatccca aaatttctag
     1861 tgattttaat ttcataaatt atttatctat ctgatccttc ttttgtttct ctaaatcttc
     1921 ttctaacgac tgccaagttt ctaaagcctt ttttgctgcc tcttgctctg ctaatctttt
     1981 tgaagtgccc tcgccttcgg agatgagctt atcacctaaa taaacaccta cctgaaaatg
     2041 tttggcatga tccggccccc actcatttag tgttttataa accggggtta cattaatttc
     2101 agcttgagct ttttcttgaa gtaagctttt tgcgtctcta tacctccctt cttttaaaat
     2161 ttcctctaaa taaacattga tgagattttc ttcaacgaat tgctgacatt ttttataacc
     2221 actatctaaa taaatcgcac ccaaaagcgc ttcaaaggta ttggctaaaa tgtaacgacg
     2281 gccaaggcct aaatcttttg cttcacctct ggataaaagc aagtatttat taaaatttaa
     2341 attattagct gcatcagcca acgtttcggc atttaccaaa ctagcccgca ggcttgttaa
     2401 aaatccttcg ggctcgttgg gaaatttttt aaacagatat tcactgacca ctaattccaa
     2461 aacagcgtcg cccaaaaatt ctagacgctc gttgtgcccc aatcgccatc gaggattctc
     2521 gtttaaataa gagcgatggg aaaaggcctg ttgcaataaa tcaatatttt tgaattttat
     2581 tttgatattt tcttctaatt cttcaaattt gtccattgcg taatgaaaaa ttttaaaaga
     2641 gggaataaaa aattattttt gctctaacaa agaatcacct tctatatttt tttcgttttg
     2701 catcgttgtt tcgttttctt cttcttcagc ggaattttgc gaaacttctt tctttgattt
     2761 cggcactgac tctttaatta ctccttcctc taccaactga cggtatattg ttcctaaaac
     2821 accatttaca aatttacttg atgattcgct gccaaaagtt tttgccaact ctaccgcttc
     2881 attgatagcc acttttggcg gcacttcatc tttaggaccg aaaataagtt cggaaatacc
     2941 caatcgtaaa atattgcggt caatcaaatt aatctgatcc agcggccact caggagccgt
     3001 tttttgaata atattatcaa tctccgtaaa atgatcaact aaatttttcg ctaaacgata
     3061 aacaaaatct ggttcatcaa taaaagaaag tgcctctttc atattgcgat caatagtcgc
     3121 catcaaatca atatcgcgct ttaaattagc tcgattaaaa tcccattcat aaagagattg
     3181 taaaagaatt gaacggcaga gatggcgcgt agccataaat tattttttta tttccttcgc
     3241 tcgactcttt ttttcttttt tggaaagttt ggctaagacg tccaccactt ctttttgagc
     3301 ataatagcca caattggggc aatagcgatg cggtattatt gccgctccac atttgggaca
     3361 ggcaataaaa ccaccagatt gaatttttaa gtgcgagcgt ctgcgtctta ctttcccatg
     3421 cgttgaatgt ttggtaggta caccacccat aaaaatctac ttctttatct attaattcga
     3481 cttattctat cttaacgcat caaagatttt tttgcaagaa gaaaaaatac taaattcaaa
     3541 atgctcgtct gatattgagc aaaacggtac taattcaaaa ttccgtctgc cacaacttgt
     3601 taaatattat tattgcagag ataatcatcg tttggttaat tagtgaagtg aggaatctga
     3661 agggattttt attgtcattc tacttttttg ccgtcatccc gttttttaat tgtcatc
//
LOCUS       DAOSMC010000032         3542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_28673_length_3542_cov_18.478348, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000032 DAOSMC010000000
VERSION     DAOSMC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28673_length_3542_cov_18.478348"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..2714
                     /locus_tag="PL075_02625"
     CDS             108..2714
                     /locus_tag="PL075_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOD97102.1"
                     /translation="MEKANWHHLYINDVAQILNTDLQRGLSEEEAKNRLKKYGFNKLP
                     EEKPLSRLKILLDQFSSPLVFILVIAGIICLIINETTDAIVIFSAVVLDVVVGFFQEN
                     KASQILMSLKKVIKHEAEVLRDGHLKVIDSTLIVPGDIVILNAGDKVPADGLLFEAYE
                     LTTNEAALTGEWLSAHKAPGRLPQSTPLADRDNMVYMGTIVENGKGKFVVTETGINTE
                     IGKIARMIQETKEEKTPYQKKLANFSKIVGIIILFICAGIFIEGMLTGGNFVEMFVTS
                     IAIAVASIPEGLPVAMTVILAVGMQRILKKGGLVKRLASAETLGSTSIIATDKTATLT
                     EGKMTLKEIIPTMSNAKSEEENNKVILKAAALTSEAFIENPEEAKENWIIRGRPTDKA
                     LLEAGIEAGINKQDFEKYKLLELPFNSKRKYNGAIYSENRQQVLYICGAPEKLLNVCN
                     LNEEERKQLEKKLEELTKKGLRIVSTAYKTLENIDLNKIKIEEELKNLTFMGFISLED
                     PIRKEVKDAMKICRQAGMKPIIVTGDHKLTAMAVAKELGFEIKEKNILEGKDLDKLSD
                     IEFERVLDDIQIYARVEPRHKMRIVAAWQKRGEVIAMVGDGINDAPALKKADIGVAVG
                     SGTEVAKEAADLILLNDSFSIIVAAVEEGRGILDNIRKVVTYLMSDSFTEAILIGVSI
                     FVGTPLPITAVQILYINLIEDGLPDMALAFEPKEKDLMQQKPQRHDVPLLTKEMRDII
                     FIVGLITDLILLGTFFFLLQQNYELDYLRTIIFAMLAIDSISYVFCCKSLRKNIWQTN
                     LLNNKLLLGAWAFGIVALIAAIYFPPLNVFLKTTPLHLDSWKIIIGMGLINIVLIEFV
                     KWIFIRNGKTQE"
     gene            2771..3055
                     /locus_tag="PL075_02630"
     CDS             2771..3055
                     /locus_tag="PL075_02630"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOD97103.1"
                     /translation="MKQQFKEKVYRVVFSISKGKVLSYKEVAKKAGNSNACRAVGNIL
                     NKHKIKKLPCHRVIKSNGEVGGYRWGSKKKIALLKKEGVKIDTKGRIIIK"
     gene            complement(3298..3369)
                     /locus_tag="PL075_02635"
     tRNA            complement(3298..3369)
                     /locus_tag="PL075_02635"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3335..3337),aa:Gly,seq:gcc)
     gene            complement(3397..3456)
                     /locus_tag="PL075_02640"
     tRNA            complement(3397..3456)
                     /locus_tag="PL075_02640"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
BASE COUNT         1319 a          483 c          675 g         1065 t
ORIGIN      
        1 ttcccatcat aatcagtgaa aaatagccga agttcttttt gaaaactatt ttaaaaatag
       61 taaaataaga ataatataga caaataatta aagtaaattc aaaaattatg gagaaagcca
      121 actggcatca tttatatata aatgatgtag cccaaatttt aaatacagat cttcagcgag
      181 gcttatcaga agaggaagca aaaaataggt taaaaaaata tggctttaat aaattacccg
      241 aagaaaagcc tttatctcgt cttaaaatat tactcgatca atttagcagt ccgctagttt
      301 ttattttagt tattgctgga attatttgtt taattattaa tgaaacaaca gatgctattg
      361 taatttttag cgcggtagtt ttagatgttg ttgttggatt ttttcaagaa aataaggcca
      421 gtcaaatttt aatgtctttg aaaaaagtta taaaacacga agctgaggtt ttaagagatg
      481 gccatttaaa agttatagat tctactttaa tagttcccgg agatattgtg attctaaatg
      541 ctggcgacaa agtaccggct gatggattac tatttgaagc ttacgaatta accactaatg
      601 aagcagcttt aacaggagaa tggttatcgg ctcataaggc gcctggccgt ttgccgcaat
      661 caacaccttt agccgataga gataatatgg tttatatggg aaccatagtt gaaaatggta
      721 aaggaaaatt tgtagtaaca gaaacaggta taaatacaga aattggcaaa attgccagaa
      781 tgattcaaga gaccaaagaa gaaaaaactc cttatcaaaa aaaactggct aatttttcta
      841 aaatagtagg aattataata ttatttattt gtgcaggtat ttttattgaa ggaatgctaa
      901 ccggtggaaa ttttgtagaa atgtttgtaa catcaatagc gatagctgta gcgtctattc
      961 cagaaggatt gccggtggca atgactgtta ttttggccgt aggaatgcag aggattttaa
     1021 aaaaaggagg gctggtaaaa agattggcct cggctgaaac attaggaagc acttcgataa
     1081 ttgccaccga caaaaccgcc accttaaccg aaggaaaaat gaccctaaaa gaaataattc
     1141 ctacgatgtc taatgctaaa tccgaagaag agaataataa agttatatta aaagcagcag
     1201 cgctcacatc agaagccttt attgaaaatc cagaagaagc aaaagagaat tggattatta
     1261 gagggcggcc aaccgataag gcattattag aagctggtat tgaggcagga ataaataaac
     1321 aagattttga aaaatataaa ttattagagt tgccgtttaa ttcgaaacgc aaatataatg
     1381 gagcaattta tagtgaaaat agacaacagg ttttatatat ttgtggagcg ccagaaaaat
     1441 tattaaatgt ttgtaattta aatgaagaag aaagaaagca attagaaaaa aagttagaag
     1501 aattaaccaa aaaaggatta aggattgtct ctaccgctta taaaacttta gaaaatatag
     1561 acctcaataa aattaaaata gaagaggaac taaaaaactt aacctttatg ggttttatta
     1621 gtttagaaga tccaattaga aaagaagtca aagatgcaat gaaaatttgt cgacaggctg
     1681 gtatgaagcc aattattgta actggcgatc ataaactaac agcaatggca gtggcaaaag
     1741 aattgggatt tgaaataaaa gaaaagaata ttttagaagg aaaagattta gataaattat
     1801 ctgatataga atttgaacga gttttagacg acatccaaat ttatgcccga gtagagccga
     1861 gacataaaat gagaattgtt gctgcttggc aaaaaagagg agaggtaata gcaatggttg
     1921 gtgatggaat taatgatgct ccggctttaa aaaaagcaga tataggagta gctgtgggat
     1981 caggcaccga agtagcaaaa gaagcggctg atctaatttt attaaatgat agtttttcaa
     2041 ttattgttgc agcagtagaa gaaggaaggg gcattttaga caatatcaga aaagttgtta
     2101 cttatttaat gtctgattct tttactgaag cgattttaat tggagtaagt atttttgttg
     2161 gaactccttt gcccatcacc gctgttcaaa ttttgtacat aaatttaata gaggatggtt
     2221 taccagacat ggctttggct tttgagccta aagaaaaaga tttaatgcaa caaaaacccc
     2281 agcgacatga tgtaccttta ttaaccaaag aaatgagaga tattattttt attgttggtt
     2341 taattactga tttaattctt ttgggtacct ttttctttct tcttcaacaa aattacgaac
     2401 ttgattattt aaggacaata atctttgcga tgttagcaat tgattctatt agttatgttt
     2461 tttgctgtaa aagtttaaga aaaaacattt ggcaaacaaa cctcctcaat aataaacttt
     2521 tattaggagc ttgggctttt ggcattgtgg ctttaatagc tgcaatttac ttccctcctt
     2581 taaatgtatt tttaaaaacc acaccccttc atttagatag ttggaaaatt atcattggaa
     2641 tgggattgat aaacattgtc ttaattgaat ttgttaaatg gatttttatt cgtaatggga
     2701 aaactcaaga ataaaaaata aattttttaa aaaagttctt attttcccat aaaagagtta
     2761 aaaacaaaca atgaagcaac aattcaaaga aaaagtttat cgagtggttt tttctatttc
     2821 taaaggaaag gttttaagtt ataaagaagt agctaaaaaa gctggtaatt cgaatgcctg
     2881 ccgtgcggta ggtaatattt taaataagca caaaattaaa aaattgccgt gtcatcgtgt
     2941 tattaaatcc aatggagaag ttggtggata tcgttggggt agcaagaaaa aaatagcgct
     3001 tcttaaaaaa gaaggcgtaa aaattgatac aaaaggaaga attattatca aataaacagc
     3061 aacttattgt tcagttagga tgattttgat aacacaagga aaaattctta aaataagaaa
     3121 acctcgcgtg ttattagatt gtattaaata aaaacataat taccgcgatg ataaaaaagc
     3181 agaactttta tagttttttt atttttgctt tttgatatcg aaaataaaaa cgacagttct
     3241 taaaactatt taaaaacaac agaaacgttg tttagagttt aactattata aataatgagc
     3301 gggagacggg acttgaaccc gcgaccttct ccttggcaag gagacgttct gcctctgaac
     3361 tactcccgca gaactataag agtataatta atgtgcctcg ggagggaatc gaacccccga
     3421 cgctcccgat gcttcgggac gctctaccac taagccgttt tattgtttta ttaaagaggc
     3481 aattaattaa ttaatgtgcc tcgggaggga atcgaacccc cgacgctccc gatgcttcgg
     3541 ga
//
LOCUS       DAOSMC010000033         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_31020_length_3338_cov_18.597015, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000033 DAOSMC010000000
VERSION     DAOSMC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31020_length_3338_cov_18.597015"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1069)
                     /locus_tag="PL075_02645"
     CDS             complement(173..1069)
                     /locus_tag="PL075_02645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOD97104.1"
                     /translation="MIPFFKSNDKSFTLLKGDCVELLNQFDFKFDMIFADPPYFLSND
                     GISVQSGKMVSVNKGEWDRSNGFEKDNEFNLNWLKACKEHLTENGTIWISGTFHNIFS
                     VAQMLTELDYKILNCITWAKTNPPPNLSCRYFTHSTEFIIWARKSKKSSHFYNYELMK
                     TINGGTQMKDVWNLPAIAPWEKSCGKHPTQKPLALLTRIILASTEKNGWILDPFTGSS
                     TTGIAANLANRKFLGIDKVDEFLSISKNRKLEIENPQIFGTYRKKLGGFEDKNQLELF
                     LAKEPEIEYERSLNLDKTTASP"
     gene            complement(1078..1347)
                     /locus_tag="PL075_02650"
     CDS             complement(1078..1347)
                     /locus_tag="PL075_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019082419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97105.1"
                     /translation="MACKKDHSRIQTIMANLPIDQGGIGRHKCAACAYDEGFNAGYNL
                     NEQINLNNLLDSLEESQAKEQRHKSPHAAYAQGYLDGVNQYYSER"
     gene            complement(1357..2196)
                     /locus_tag="PL075_02655"
     CDS             complement(1357..2196)
                     /locus_tag="PL075_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HOD97106.1"
                     /translation="MSEQFETFISQLSETNATLDYFTDFGKIKTNLSKISIKLNQLNY
                     LIGKEDLKLAIEELFEENAKVFEVLDILIAVRKNKNAKTFNREGEIVQLDTYFETSDK
                     IYEFIEDTGLGEVFRNKNITNLVDYVFGVEVGLDTNARKNRGGENMSKAVSLIFDKAK
                     IFYKKEVNSTEFPEITSLGADLKRFDFVIKTKVKTYLIETNFYNGGGSKLNEVARAYS
                     DVAPKINQYENFEFVWITDGQGWLSAKNKLEEAFSIIPSVYNLSNLDSFTQKITKEVV
                     LTF"
     gene            complement(2180..3100)
                     /locus_tag="PL075_02660"
     CDS             complement(2180..3100)
                     /locus_tag="PL075_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOD97107.1"
                     /translation="MTIKAKPFIKWVGGKTQLIESIEKALPENFAKNKNVTYIEPFVG
                     GGAILFWILQQYPNITKAVINDINPDLTTAYKTIKETPTELVKYLQIIQDEYLPLDEE
                     KRKEYYLNKRERFNTKSLDSIENTALFIFLNRTCFNGLYRVNSKGLFNVPFGRYANPK
                     ICDKQTIFADSELLQKVEILTGDFEDTLKYATNNSFFYFDPPYKPLSETSSFNSYAKE
                     DFNDLEQIRLANFCKKIDLLGHDFILSNSDVKGKNPNDDFFDDLYEQFSIKRVFATRM
                     VNANAEKRGKLTELLITNQPSEELEYVRAI"
BASE COUNT         1008 a          591 c          474 g         1265 t
ORIGIN      
        1 atacgtgcaa atatatttaa ataaacacgt gtcaatcttt ggtttgcccc taacgcttgc
       61 gtgtatgaaa cgtttggcat ttcgaagcga tttcctatca agttacaact acttttgata
      121 cgagcagtac cgcttgaaaa cccactgact gccaaatgtt ttatacacgt tgttatgggc
      181 tggctgttgt tttgtctaaa tttaaacttc tttcatattc aatttctggt tctttcgcta
      241 aaaacaattc tagttgattt ttatcctcaa atccaccaag tttttttcta taagtaccaa
      301 atatttgtgg attctcaatt tcaagttttc tgtttttaga aatactcaaa aattcatcga
      361 ctttatcaat tcctagaaat tttctatttg caagatttgc agcaattcct gttgtcgaac
      421 tacctgtaaa tgggtctaaa atccaaccat ttttttcagt agatgctaaa ataattcttg
      481 tcagcaatgc taaaggtttt tgtgttggat gttttccaca agatttttcc caaggagcaa
      541 ttgcgggcaa attccaaaca tctttcattt gagttccacc gttaatggtt ttcattaact
      601 cataattgta aaaatgagag gattttttgc tttttctcgc ccaaattatg aactctgtag
      661 aatgagtaaa atagcgacaa gaaagattag gcggaggatt tgtctttgcc catgtaatgc
      721 aattcagaat tttataatcc aattctgtaa gcatttgagc tacggaaaaa atattatgaa
      781 atgtaccact tatccaaata gtaccgtttt cagttaaatg ctctttacaa gctttcagcc
      841 aatttaaatt aaattcatta tccttttcaa atccattcga tcgatcccat tcacctttat
      901 ttacacttac cattttgccg ctttgaacag aaattccgtc atttgataga aaatatggag
      961 gatcagcaaa tatcatatca aacttaaaat caaactgatt aagaagttca acacaatcgc
     1021 cttttaacag tgtaaaagat ttatcattag atttgaaaaa tggaataata gccacgatta
     1081 tcgctcagaa taatattgat ttacaccgtc aagataacct tgtgcgtatg cagcatgagg
     1141 gcttttatgt ctttgttctt ttgcttgact ttcttctaaa gaatctaata aattatttaa
     1201 atttatttgt tcattaagat tatagccagc attgaaacct tcgtcataag cacatgcagc
     1261 acatttatga cgaccaatac caccttgatc aattggcaaa tttgccatta tagtttgaat
     1321 tcttgaatga tcttttttgc aagccataat ttttcgctaa aaagttaata ctacttcttt
     1381 tgtaattttc tgagtaaatg aatctaagtt tgaaagatta tacacacttg gtataattga
     1441 aaatgcttct tctaatttgt tttttgcaga taaccagcct tgtccgtctg taatccacac
     1501 aaattcaaaa ttttcgtatt gatttatttt gggggcaaca tcagaatacg cgcgtgcaac
     1561 ttcattaagt ttagaaccgc cgccattata aaaatttgtt tcaatcaaat aggttttaac
     1621 cttagttttt atcacaaaat cgaatctttt taaatctgca cccaagcttg ttatctcagg
     1681 aaactcagtg ctgtttactt cctttttgta gaagattttc gctttatcaa aaattaatga
     1741 aacagctttt gacatatttt caccgcctct gttttttctt gcatttgtgt caagtcctac
     1801 ctcaacacca aaaacataat caacaagatt tgtaatattt ttatttctga atacctcacc
     1861 taatccggta tcttcaataa attcatagat tttatctgaa gtttcaaagt acgtgtcaag
     1921 ctgaacaatt tcaccttctc tattgaatgt ttttgcattc ttgttttttc taacagcaat
     1981 caaaatatct aagacctcaa aaaccttcgc attttcttca aataattctt caattgcaag
     2041 ttttaaatcc tcttttccta tcaaatagtt gagctgattt agtttaattg aaattttact
     2101 taaattggtt ttaatttttc caaaatcggt aaaataatca agggttgcgt tggtttcact
     2161 taattgtgaa ataaaagtct caaattgctc tgacatattc taattcttct gatggttggt
     2221 tggtaattaa taattctgtt agttttcctc gtttttctgc atttgcattc accatcctag
     2281 ttgcaaaaac ccttttgatg ctaaattgct catataaatc gtcaaaaaaa tcatcgttag
     2341 gatttttacc ttttacatca gaattactta aaataaaatc atgtccaagt aagtcaattt
     2401 ttttacaaaa attagctaat cgaatttgtt ctaagtcatt gaaatcttct tttgcatatg
     2461 aattgaaact tgaagtttca cttaacggtt tatagggagg atcaaaataa aagaaactat
     2521 tatttgtagc atattttaat gtgtcctcaa aatctccagt caatatttct actttttgca
     2581 gaagttcgct atctgcaaaa attgtttgtt tgtcgcaaat tttaggatta gcgtatcgtc
     2641 caaatgggac attgaaaagc cctttactgt ttactcgata taagccattg aaacaagtac
     2701 ggtttaaaaa gataaataat gccgtattct caatggagtc aagtgatttt gtgttaaacc
     2761 gttccctttt gttcagatag tattctttcc gtttttcttc atccaatggc aaatactcat
     2821 cttgaattat ttgaagatat ttaactaatt cggtcggtgt ttctttaatt gttttataag
     2881 cagttgttaa atctggattt atgtcattaa taaccgcttt tgtaatattc gggtattgtt
     2941 gtaaaatcca gaataaaata gctcctccac caacaaaagg ctctatataa gtaacatttt
     3001 tatttttagc aaaattctca ggcaaagcct tttcaatgct ttcaattagt tgggtttttc
     3061 cgccaaccca tttaatgaat ggttttgctt ttatcgtcat atttattttc tttcttgcaa
     3121 atttacaatt ttattttcgg tttgcagtat gctgtctgcg tgcgttttct cagcttgccc
     3181 ataacaaggg ttttgcgtaa tatccctatc aagtgtcgtg attaatttta agtttttact
     3241 aaggcctact acgcaaagcc ccgatacgtt atacccaaaa gccttgcagc ttcgtctgat
     3301 tttagaattt ctttcccttc gaattcattt gttttttc
//
LOCUS       DAOSMC010000034         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_32036_length_3257_cov_18.644909, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000034 DAOSMC010000000
VERSION     DAOSMC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32036_length_3257_cov_18.644909"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1705
                     /locus_tag="PL075_02665"
     CDS             38..1705
                     /locus_tag="PL075_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOD97108.1"
                     /translation="MNDKSHIIQNILPILLLIVVGFFIYFNSFGNQMFWDDNDGILNN
                     AYIKNWQYWPKFFTENLIAGSGLVSNYWRPMLLTVFSFEWHLWQDNVFGYHLINTLFH
                     IFNAILLFLILKICLKKKWPVLFLTLIFLVHPAQTEAVTYVSGLADPLSAFFIFLGIF
                     FYLQFLQKNKKVFYWLSALSYPLALMSKETAIIMPLLIFLFEWLYKRGEHFSFAEIIK
                     KYGPFILIAIIYIVLRLTILNFQNTLNLYNQEDLFTSRLDIRILTFLKTLPIYLGLIF
                     YPISLHMERTIEIPTSLADPLVLGGIAIVLISIFLIVMCFRRRPEISFGLLWFFIGLA
                     PVSNILVPVSGLIYEHWLYLPFIGIFIAVYYLIEKSITQYHLEKLLLIVVIVLVIFFS
                     WRTILRNAEWRDPITFYKQTLQYTPESYRIWNNLGMAYDDNGQYLLAEKAYQTATSID
                     PDNPIAYHNLGNLYFKLNRLDDAEKYLLEAIKNDSTFHYSYISLARLYLAQGNLTHAK
                     NILQKYLETNSPRADVLLLLSNIYENEGDFANAQKYLLEAQKLQLQK"
     gene            1728..2429
                     /locus_tag="PL075_02670"
     CDS             1728..2429
                     /locus_tag="PL075_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOD97109.1"
                     /translation="MKKLSIIIPVFNEAETIQKILRFIAVLPLHWEKEVIVVDDGSTD
                     GTSEILEKLNQEAQSFIYVKHSKNFGKGRAIRTGLQYVTGNAVIIQDADLEYDPFDYQ
                     KLLAEFEKGNKVVYGSRNLDTKTERGYFFYFLGGRLITAICNLLYRSHLTDINTGYKL
                     FDAALLKNLNLEKDGFEFCEEVTAKILKKGIPIKEVSINYYPRSFQEGKKIKIKDGLK
                     AIETLLIYHFGKKEL"
     gene            2440..3231
                     /locus_tag="PL075_02675"
     CDS             2440..3231
                     /locus_tag="PL075_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOD97110.1"
                     /translation="MKTKRKKVGLALGSGGLKGFAHVGVIKVLEENNIPIDFIAGSSI
                     GALVGGLYAANKDIKQLEEIIFTTDVKKGLGLLDPRLSKGFLRGRKIEEFVNHYLYRK
                     NFDDLKIPLAVVATNLRTGEAEIIQNGDLSSAIRASISVPIIFQPIKREGKLLIDGGM
                     TMPIPIKVLKQMGADIVIAVNLYNAFERPKRFPKISMSLIGNETLEIMLYQITKRDIV
                     EADVVIAPDVDGYSVIKALEQRQKIFHLGEEAAREKINLIKELIS"
BASE COUNT         1019 a          531 c          562 g         1145 t
ORIGIN      
        1 attttagaaa aattaaaagt ttgctaaaat aatcttaatg aatgataaat cccatattat
       61 tcaaaatatt ttgccgattc ttttactgat tgttgttggc ttctttattt atttcaatag
      121 cttcggcaat caaatgttct gggatgataa tgacggtatt ttaaataacg cctatatcaa
      181 aaattggcaa tattggccga aattttttac agaaaattta attgctggct ccggccttgt
      241 gagcaattat tggcggccca tgcttttaac tgttttttct tttgaatggc atctctggca
      301 agataatgtt tttggttatc atttgatcaa tacgcttttt cacattttta acgccatttt
      361 gctttttctt attttaaaaa tctgcttaaa gaaaaaatgg ccagtccttt ttctgacttt
      421 aatttttctg gttcatccag ctcaaaccga agctgtcact tatgtttctg gtctcgcgga
      481 tccactatcg gcttttttca tttttctcgg catctttttt taccttcaat ttctgcaaaa
      541 aaataaaaaa gttttctatt ggctgtcggc gctttcttat cctctcgcac taatgagcaa
      601 agaaaccgct attattatgc ctcttttaat ttttctcttt gaatggcttt ataaaagagg
      661 agagcatttt tcttttgcgg aaattattaa aaaatacgga ccatttattc ttattgctat
      721 tatttatatt gttttgcgct taacgatttt gaattttcag aacactctta atttatataa
      781 tcaagaagat ttatttactt cgcgccttga tattcgcatt ctcacatttt tgaaaacatt
      841 gccaatttac ttaggattaa tattttatcc tatctcgctt catatggagc gaacaattga
      901 aattccgact tctttagccg atcctctagt gctgggtggc attgccattg tgttgatctc
      961 tatttttttg attgtgatgt gtttccgtcg tcgtcctgaa atttcttttg gtctcttgtg
     1021 gttttttata gggctagccc ctgtttctaa cattttggtg cccgtgagtg gtttaattta
     1081 tgaacactgg ctttacctcc cattcattgg cattttcatt gctgtttatt atctgattga
     1141 gaaatccatt actcaatatc acttagaaaa attactatta attgtggtga tcgttttggt
     1201 tatctttttt tcttggcgaa caattttaag aaatgctgaa tggcgtgatc ctattacttt
     1261 ttacaaacaa acgttacaat acacgccaga aagttatcgt atttggaata atttaggaat
     1321 ggcttatgat gataatggcc agtatctttt agctgaaaaa gcttatcaaa ccgctacaag
     1381 cattgatcct gacaatccca tcgcttatca taatctaggc aatctctatt ttaaacttaa
     1441 tcgattggat gatgctgaaa aatatttgct tgaagctatt aaaaacgatt caacatttca
     1501 ttattcttat atttccctcg cccgacttta tttagctcaa ggcaacctca ctcatgccaa
     1561 aaatattctc caaaaatatt tagagacgaa cagtcctcgc gccgacgtcc tattactctt
     1621 atcaaatatt tatgaaaatg aaggcgattt tgctaatgct cagaaatatt tacttgaagc
     1681 ccaaaaatta caactacaaa aataaattct aaaaataaat ttataaaatg aaaaaactca
     1741 gcattattat tcctgttttt aacgaagcag aaactatcca aaagatttta agatttattg
     1801 cagtgttgcc ccttcattgg gaaaaggagg ttattgtggt tgacgacggt tcaacggatg
     1861 gtaccagtga aattttggaa aaattaaatc aggaagcgca gtcatttatt tatgtaaagc
     1921 attctaaaaa ttttggcaaa ggaagggcta ttcgaacagg ccttcaatat gtcactggta
     1981 atgcagttat tattcaagat gctgatttag aatacgaccc ctttgattat caaaaacttt
     2041 tggcggaatt cgaaaaaggc aataaagtag tttatggttc gcgtaatcta gatacaaaaa
     2101 ctgaacgtgg ctattttttt tattttttag gcggtcgtct gatcactgct atttgcaatc
     2161 ttctttatag gtcgcattta accgatatca ataccggtta taaattattt gatgcagctc
     2221 ttttaaaaaa tttaaattta gaaaaagatg gctttgaatt ttgtgaagaa gtaacagcaa
     2281 aaattttaaa aaaggggatt cctattaagg aagtttctat caattattat ccgcgtagtt
     2341 ttcaagaagg aaaaaagatt aaaataaaag atggattaaa ggccattgag acacttttaa
     2401 tttatcattt tggcaaaaaa gagttataat tgttacaata tgaaaacaaa aaggaaaaaa
     2461 gttggtttag cattaggaag cggcggactc aagggatttg cgcatgttgg cgtaattaaa
     2521 gttttagaag aaaataacat cccgattgat tttattgctg gctcaagtat cggcgctttg
     2581 gtcggtggcc tttatgctgc caataaagac attaagcaat tagaagaaat tatttttaca
     2641 actgatgtta aaaagggatt aggattactt gatcctcgtt tatcaaaagg ttttctgcga
     2701 ggccgaaaaa tagaagaatt tgttaatcat tatctttatc gcaaaaattt tgatgatctc
     2761 aaaattcctt tagcagtagt ggcgacgaat ttacggacag gagaagcgga aattatccaa
     2821 aatggcgatt taagttctgc cattcgagct agcatttccg tgcccattat tttccaaccc
     2881 attaaaagag aaggcaaatt attaattgat ggcggaatga ctatgcctat tccgattaag
     2941 gttctcaaac aaatgggcgc ggacattgtg attgcagtta atctttacaa tgccttcgag
     3001 cgcccaaaaa gatttccaaa aatttcaatg tcactcattg gcaatgaaac attggaaatt
     3061 atgctttatc agattaccaa aagagatatt gttgaagctg atgtggtgat tgcgccggat
     3121 gttgatggtt atagcgtcat taaggctctg gaacagaggc aaaaaatttt tcatttgggc
     3181 gaagaagccg ctcgagaaaa aataaactta ataaaagaat taatcagtta aaaaataaca
     3241 ttttattgtc atcctga
//
LOCUS       DAOSMC010000035         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_33259_length_3167_cov_17.920308, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000035 DAOSMC010000000
VERSION     DAOSMC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33259_length_3167_cov_17.920308"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PL075_02680"
     CDS             complement(<1..818)
                     /locus_tag="PL075_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOD97111.1"
                     /translation="MDQKITKEEAKKRIEKLRKEINYHRYLYHVLDKEEISPEALDSL
                     KKELFDLEQQFPDLITPDSPTQRIGGKPLKEFKKVKHPTPMLSFNDCFSEDDMRAWEK
                     RFMDFLGGKVHRSDKFYYCELKFDGLAIELHYKDGIFVCGATRGDGYVGEDVTQNLKT
                     IEAIPLRLLEKEEVIANLRKEGLNHIVARLEKNFPREIIARGEVFMNRKDFEALNKEQ
                     VKKHQKPFANPRNAAAGSVRQLDPTITASRKLNSHAYSLVTDLGQKTHEEEHLI"
     gene            complement(842..1201)
                     /locus_tag="PL075_02685"
     CDS             complement(842..1201)
                     /locus_tag="PL075_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97112.1"
                     /translation="MKTQQLKQLKNQKGQAMLITMLILMSSFLVSMALGGLVLYELKS
                     MIFTGESVKALYAAESGLEWKLYQANHPGEEDGREPTMNNGTSFIVSVSSTTEGYLVI
                     YSVGRSINTYRALEVMY"
     assembly_gap    1314..1413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1492..2037)
                     /locus_tag="PL075_02690"
     CDS             complement(1492..2037)
                     /locus_tag="PL075_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOD97113.1"
                     /translation="MAIVYAAVGKRKSKMKNKNEKIKNNKGFSLVEVMVSIGIILVAF
                     TGVLTLINRSVAFHDLAYSRLTASYLAQEGIEIVRNIRDNNIITEKRWNDGLTAGVYQ
                     VQYNSSILTPYTGEYLLLDPVRGLYTYEANNNTRQTRYARRIEIQVISPDEIRVNSIV
                     EWSNRGGEFEINVEDHLFNWI"
     gene            complement(2045..2563)
                     /locus_tag="PL075_02695"
     CDS             complement(2045..2563)
                     /locus_tag="PL075_02695"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOD97114.1"
                     /translation="MRRNNQGFTLIEMLVVTTIVILLSGLLILYSREGDKVSLILRAR
                     TQVIADINRAKNLAITAQSWNGEHTCGYGVYFDTANNRYIIFTEVASNCESSDHVYQR
                     GSYDVEIIPVTNQFVLSSANISQVFFMPPDPTVFFDPPEMEQAEINFNLVNQANPAFQ
                     IFINSLGQVWGI"
     gene            2696..2821
                     /locus_tag="PL075_02700"
     CDS             2696..2821
                     /locus_tag="PL075_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97115.1"
                     /translation="MDEQNQNPTPQATAEKPKKSNAWIWVVIVIIIILAIILLAL"
     gene            2824..>3167
                     /locus_tag="PL075_02705"
     CDS             2824..>3167
                     /locus_tag="PL075_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97116.1"
                     /translation="MNEAPVQQPKKGGKIWHWILTIILIIVAALLGYTAGQNAAVQPT
                     VSVEKATTLEQNYGDVPPEIFPQDFPFEVGTLLVQSLEKTDKETGITHASVAYYSQKS
                     IQENADKFIAYL"
BASE COUNT          963 a          612 c          487 g         1005 t
ORIGIN      
        1 aaaataagat gttcctcttc gtgagttttt tgacctaaat cagtaacaag agaataggcg
       61 tgtgaattca atttgcgact cgccgtaata gtaggatcta attgtcttac tgagccagcc
      121 gcggcattgc gaggattagc aaatggtttc tgatgttttt taacttgttc tttgtttagt
      181 gcctcgaaat ctttgcgatt cataaaaacc tctccgcgag caataatttc acgcggaaaa
      241 tttttttcga gtcgagcgac aatatgattc aaaccttctt ttctcaaatt agctataact
      301 tcttcttttt ctaataaacg cagaggtatg gcttcgatgg tctttaaatt ttgggtcaca
      361 tcttcaccga catatccatc accacgagtg gcaccacaaa caaaaattcc atctttgtaa
      421 tgcagctcaa tagctaaacc atcaaatttt aattcacaat aatagaattt atcagagcga
      481 tgcactttac cgcctaaaaa atccataaat cttttttccc aagcgcgcat gtcatcttca
      541 gaaaaacaat cattaaaaga aagcatcggc gtcggatgct ttactttttt aaattctttc
      601 aatggcttac cgccgatccg ctgcgtaggt gaatcgggag tgattaaatc aggaaattgt
      661 tgctctaaat caaagagttc cttttttaaa gaatctaaag cttccggcga aatctcttct
      721 ttatctaaaa catgatatag ataacggtga taatttattt cttttcttaa tttttcaatt
      781 ctttttttag cctcctcttt agtaattttt tgatccatac acgaagggta ttatagaacg
      841 cctaatacat cacttcaaga gcccgatatg tattgatgct ccgaccaact gaataaatca
      901 ctaaataccc ttcagtagta gaagaaacgc tgactataaa actggtgcca ttgttcatag
      961 ttggctcacg accatcttct tcacccggat gattagcttg ataaagcttc cactccaaac
     1021 cactttccgc tgcataaagt gctttgactg attcgccggt aaagatcatg gattttaatt
     1081 cgtataacac caatcccccc aaagccatac taaccaaaaa tgatgacatt aaaataagca
     1141 tagtgattaa catcgcttgt cctttttgat tttttaattg tttcaattgt tgcgttttca
     1201 ttattttaac tttcaggttg acgactgctt atagttgtct ctaaattcgt ttctaattgt
     1261 ttggcgcctg cataacttgc aattcttaaa agaatagtca cccgcacttg ctgnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcagaa ataaagccga agcaactaat
     1441 gtttctacca tcgtaaatcc atttttagtt tggcaatcat tttttttcat cttaaatcca
     1501 attaaaaaga tgatcttcga cattaatttc aaactcccca ccacgattgc tccactcaac
     1561 aatagaatta actctaattt catcaggcga aataacttga atttctatcc gccgagcata
     1621 acgcgtttga cgagtattgt tattagcttc gtaagtatac aaacctcgca ctggatcaag
     1681 caataaatat tctcctgtat aaggcgttaa tatagaagaa ttatattgaa cttgataaac
     1741 gcctgctgtt aaaccatcat tccaccgctt ttcagttatt atgttgttat ctcgaatgtt
     1801 tctcacaatt tctattcctt cttgagctaa ataagaagcg gtcaatcgac tgtaagccag
     1861 atcatggaaa gcaactgatc gattaatcaa agtaagaaca cccgtaaaag caaccaaaat
     1921 aataccgata ctcaccatca cttccactaa actaaatcct ttattgtttt ttattttttc
     1981 gtttttattt ttcattttgc tttttcgctt acctacagca gcgtaaacaa ttgccatttt
     2041 ttatttatat tccccacact tggcccagag aattaataaa aatctgaaat gctggattgg
     2101 cttgattgac tagattaaaa ttaatctccg cctgttccat ttcaggagga tcaaaaaaaa
     2161 ctgtcggatc gggtggcata aaaaatacct gagaaatatt agctgagctt aacacaaatt
     2221 gattagtcac aggaataatt tctacatcat aagatcctct ttgataaaca tgatcggaag
     2281 attcacaatt actggcaact tcagtaaaaa ttatataccg attgttagcc gtatcaaaat
     2341 aaacgccgta accgcaagta tgctcgccat tccaagattg agcggtgatg gctaaatttt
     2401 tagctcgatt aatatcagca atcacctgag tgcgggctct tagaattaaa ctcactttat
     2461 cgccttcacg ggaatataaa attagaagac cgcttaataa aatcactatt gttgtgacca
     2521 caagcatttc aattaatgta aaaccttggt tatttcgcct cattcttttt attttactct
     2581 tttttttagc gaataaaaag ggagttctta tccacagctc atttggttga cttttatttt
     2641 tggagtataa taaagaagag gtaaggtcga ttttaatttt tatcattcct ttattatgga
     2701 cgaacaaaat caaaatccta ctccacaagc aacggccgaa aaaccaaaaa agagtaatgc
     2761 ttggatttgg gtcgtaattg tgattattat tattttggcc atcattcttt tggccttata
     2821 accatgaacg aagcacctgt tcaacaacca aaaaagggcg gaaaaatatg gcattggata
     2881 ctgacgatta ttttgattat cgttgccgct ttgttaggct ataccgcggg acagaatgca
     2941 gctgttcaac caactgtttc ggtggaaaaa gcaactactt tagaacaaaa ttatggcgat
     3001 gtacctccgg aaatcttccc tcaagatttt ccgtttgaag ttggtacttt attggttcaa
     3061 tcgcttgaaa aaacagataa agaaacggga atcactcatg caagcgttgc ttattattca
     3121 caaaaatcca ttcaagaaaa tgccgataaa tttattgctt atcttaa
//
LOCUS       DAOSMC010000036         3068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_34688_length_3068_cov_20.692001, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000036 DAOSMC010000000
VERSION     DAOSMC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34688_length_3068_cov_20.692001"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /gene="dcd"
                     /locus_tag="PL075_02710"
     CDS             <1..501
                     /gene="dcd"
                     /locus_tag="PL075_02710"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOD97117.1"
                     /translation="IGPASIDLKLGPIFKSFNVSKHHLLDIRKGLPDKDFVITRKLKK
                     DECYILHPNCFVLAATQEYVIVPDDIILRVEGKSTLARMGILVHTAGFVDPGFEGTLT
                     LEISNQSDLPVALYPEMYICQVAVEYLSSPAEVPYNKRKKSLYSKQKEPSVAKTKNLF
                     DWTRKK"
     gene            715..2313
                     /locus_tag="PL075_02715"
     CDS             715..2313
                     /locus_tag="PL075_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOD97118.1"
                     /translation="MKNSFFISNNRGISSLVVILVTLGVIAAGSAYFLTRPRLLPTQE
                     PPVDFNQPETTILPDEIALQTEKLVMELKISSLSKSKGAVDIFWGQPGGFVGGQVQRA
                     IVTPLQLVDGIKITNGTNETVVLKEINNETRELLQNLSTGHATPILLLPEKFIKNGKE
                     EVKCQNRDLFSNSINNICVFETPIEIAPKQTLTLRPILFAVPNSLWEWDSSSISRDKI
                     TFLDGKETKNLLYITTETGKQFIFLSNAFQEYDNIAQSQTFNIGGLIFVNVNNQNLVN
                     NIFDFKTKNGQKILAEVKSGEGITFKTFELEMTSWPPENIKLAKGQFDERLLTFQVFL
                     YRNDLLDRKNITGVYDDKTAAAIKEFQKTAGIVETGELDSATIFASRGFDVGKARWEN
                     YSDEQITLSLDATQPNFRLFAIGTEGVKLNPDFSNSSLVTKVIKEPGCQESVCTINAY
                     TINGEAIFTLGSVNILNNTSQNVSCTLTHPNGKLLKSFQIAPNNFYIQNTTVEGISES
                     AIYHLQCGNKILDIELFPSTSGRG"
     gene            2386..2895
                     /locus_tag="PL075_02720"
     CDS             2386..2895
                     /locus_tag="PL075_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97119.1"
                     /translation="MLNKGSANIILIILVVVLAGAVTYLALDKEKTSPETITSFSPTS
                     IPSQSASPTQIPTSQPSQILMEQPGKIISLTSNGAEWNVTIELLKRNPEWLPGVGNEP
                     FFIDQNESLTVKASQDTKAYLCGTPSPTKDNWRPTTPGNLLKEDSTVYFDIDVSNNFI
                     TAIYQQCLP"
BASE COUNT         1042 a          525 c          542 g          959 t
ORIGIN      
        1 attgggccgg cttctattga tttaaagttg gggccaattt ttaaatcttt taatgtatca
       61 aaacatcatt tacttgatat tagaaaaggg ttgccagata aagattttgt gattacccga
      121 aaattaaaaa aggatgagtg ctatattctt catcctaatt gttttgtttt agccgcgacg
      181 caggaatatg ttattgtgcc tgatgatatt attttacgcg ttgaaggtaa aagcacttta
      241 gcgcgaatgg gcattttagt tcatacggcg ggatttgttg acccaggatt tgaaggcacg
      301 ttaactttag aaatttctaa tcaatcggat ctgcctgtag ctctttatcc agaaatgtat
      361 atttgccagg tggcagtaga atatttatct tcgccagcag aagtacctta caataaaaga
      421 aagaagtctc tttattccaa acaaaaagaa ccatcagtgg cgaaaactaa aaatcttttt
      481 gattggacaa ggaagaagta gctcatgaat taattcatga attaattcat gagctagagt
      541 acggaggctg gaaaaacttc cgtaggaagt ttttccagcc tccgtgattt tatatttcac
      601 ctgataccct ctagaaaata gaacggtaga atttaaaatt caaagcttgt ccaaaaaata
      661 tcttgtgaat aacttttttg ctaaatcgac ataaaatttg tataatgaaa acgtatgaaa
      721 aactcttttt ttatttcaaa taatagaggg atttcttctc tcgtcgttat tttagttaca
      781 cttggtgtta ttgctgctgg cagcgcttat tttttaacac ggccccggct attacccact
      841 caggaaccac cggtagattt taatcagccg gaaaccacga ttctgcctga tgaaattgct
      901 ttgcaaacag aaaaattggt gatggaatta aaaatatctt ctttatcaaa atcaaagggc
      961 gcagttgata ttttttgggg tcaacctgga ggatttgtgg gcggccaagt tcaaagagca
     1021 atcgtcacgc ccttacaatt agttgatgga attaaaatta ccaatggaac taatgaaact
     1081 gtagttttga aagaaatcaa taacgaaact cgagaattgc ttcagaacct ttcaaccgga
     1141 catgctactc ctattctttt attgcctgaa aagttcataa aaaatggaaa agaggaagta
     1201 aagtgtcaaa acagagattt atttagcaat tccatcaata atatttgtgt gtttgaaacg
     1261 cccattgaga ttgcaccaaa acaaactctc accttacgac cgatattatt tgctgtaccg
     1321 aattcattat gggaatggga ttcttcatca atcagccgcg ataaaattac tttccttgat
     1381 ggcaaagaaa ctaaaaatct tttatacatt acaaccgaaa cgggaaaaca attcattttt
     1441 ctctctaatg cctttcaaga atatgataat attgctcaaa gtcagacttt taatattggc
     1501 gggctaattt ttgttaatgt taataatcaa aatttggtaa ataatatttt tgattttaaa
     1561 acgaaaaacg gacagaaaat tttagctgaa gttaaatcgg gagaaggcat tacttttaaa
     1621 acttttgaat tagaaatgac ttcgtggcct cctgaaaata taaaattggc caaggggcaa
     1681 tttgatgaaa gattactcac cttccaagtc tttctttatc gcaatgattt gctggatcga
     1741 aaaaatataa cgggcgtcta tgatgataaa actgccgccg caattaaaga atttcagaaa
     1801 acagcaggaa ttgtagaaac aggagaatta gatagtgcaa caatttttgc tagccgagga
     1861 tttgacgtcg gcaaagcacg ttgggaaaac tatagtgacg aacaaataac attaagctta
     1921 gatgctactc aacccaattt tcgccttttt gccattggca ctgaaggtgt taaattaaat
     1981 ccggactttt ctaattcttc tctcgttacc aaagtaatca aagaacctgg ttgtcaagaa
     2041 tctgtttgca ccattaacgc ttataccata aatggagagg cgatctttac gttgggaagt
     2101 gttaatatat taaacaatac ttctcaaaat gtatcgtgta ctcttactca tcctaatgga
     2161 aaattactaa aaagttttca gatagcgcct aataattttt atatacaaaa tacaacagtt
     2221 gaagggattt ccgaatcagc aatctatcat ctccagtgtg gcaataaaat tttggatatt
     2281 gaattattcc cctcaacctc gggcagagga taaattttat gattctcttc atattaagaa
     2341 attaattatc aatctcacta aaggtcgtaa attaacatta tttctatgct taacaaagga
     2401 agtgcaaata tcattcttat tattttagtt gtagttttag ctggtgcagt gacttattta
     2461 gcactggata aagaaaaaac tagtcctgaa actattacga gtttttctcc cacatcgatt
     2521 ccatctcaat cagcttcacc aacccaaata cccacctctc aaccctcaca aatactaatg
     2581 gagcaacctg gaaaaataat ttctttaact tcaaatggtg ccgaatggaa tgtaactatt
     2641 gagctgctta aacgtaatcc ggaatggtta ccaggcgttg gaaatgaacc attttttata
     2701 gatcaaaatg agtccttaac agtaaaagcc tctcaagata caaaggctta tttgtgcggg
     2761 acaccatcgc caactaaaga taattggcgg ccaacaacac cgggtaattt actaaaagaa
     2821 gactctacgg tttattttga cattgatgta agtaataatt ttattactgc tatttatcaa
     2881 caatgtttgc cataataacg atgtttgtcg attagataaa cgattagata aaaacgaata
     2941 aggaaaaaac gaataaattt tcttctttta attagggcgt aatttgtggt tcagctcatg
     3001 aattaattca tgagctgaat agagcggctg aaaaccgtga gatccttcac taaaattcag
     3061 gatgacaa
//
LOCUS       DAOSMC010000037         2901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_37331_length_2901_cov_19.154954, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000037 DAOSMC010000000
VERSION     DAOSMC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37331_length_2901_cov_19.154954"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1417)
                     /gene="tyrS"
                     /locus_tag="PL075_02725"
     CDS             complement(155..1417)
                     /gene="tyrS"
                     /locus_tag="PL075_02725"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOD97120.1"
                     /translation="MKQNVWDELSWRGLINQTTDANKLKNRLTQPISLYCGFDVTADS
                     LHIGHLLPIITLKRFAQFNHQIIPLLGDGTSLIGDPSGKKSERQLNSEEQVEQWTIEL
                     KKQLEKIFDEEIHAQKATILQNNQWLKELKLLPFIRNIGKHFSLGYMLAKESVKSRLE
                     SGISFTEFSYMLLQSYDYLWLNEHYNCELQIGGSDQWGNITSGIDLIAKTKNKTVYGL
                     TLPLLLKSDGTKFGKTEGGTVWLNSQKTSVYQFYQYFINVSDDDVIGFLKYFSFLSQE
                     EINELEKQVKNSPEKRLAQKTLAQELTRMVHGETAFKKAEKISEALFSNQLSDLSAEE
                     IKEGLYDVPTYTISQQKEMNLIDLLVNAKICPSKRQAREDILNKAISLNGQIVTDVNL
                     KLTSQNCLIENIMVLRRGKKNYYLIYWQ"
     gene            complement(1467..2438)
                     /locus_tag="PL075_02730"
     CDS             complement(1467..2438)
                     /locus_tag="PL075_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97121.1"
                     /translation="MDLKRVFPLLDFLYAERDDLLYYLLFGDRVADEISPDDFPLIFI
                     GEDTAFIPPVIFQGPQEIHRHCFVSHSFLEHCSIKEKSRVLDSRLFHAIIGQKSVVIN
                     STFEQTSLGNNSYVNNSTISDSRIKNGVTISNGCSIEDSIIGNNVTLFPQVVVDFSRV
                     DEFSALQSGVKLVSSTGQGREPIVIEKKTYLGANVTIIGPAVIGHNSFIEAGITIHND
                     YIPPFSYVRKLMISNHYEIQPYGAFQLINGLWYIDRTRSLKLAELIQAKKKCLLFEKE
                     AEKKNINIYEKLNTIPRKRLILDHLLEKEGLNAFMHLIQNEIESLVN"
     gene            2658..2730
                     /locus_tag="PL075_02735"
     tRNA            2658..2730
                     /locus_tag="PL075_02735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2691..2693,aa:Ala,seq:cgc)
BASE COUNT          901 a          494 c          530 g          976 t
ORIGIN      
        1 attttatcaa gcatgtaaat gcatttatta aaaattaaaa ttttcttcga ttgtccattt
       61 agactttagg cttctattat ttaaataatc aaaaaattcc tcctatattc atttttgata
      121 ttaatggaga aaaaaaatat gtcaatttaa aattttattg ccaataaatt agataataat
      181 tttttttacc tcgtctcaaa accattatat tttcaattaa acaattttga gacgttaact
      241 ttaaattaac atcagttaca atttgtccat ttaaggagat ggctttattc aaaatatctt
      301 ctcgggcttg gcgtttagaa gggcaaattt tagcattaac cagcaagtca atgaggttca
      361 tttctttttg ttgggagatc gtatatgtcg gaacgtcata gagaccttct ttaatttctt
      421 cagcgctgag atcggagagt tgattagaaa acagcgcttc agaaattttt tctgcttttt
      481 taaaggcggt ttcgccgtga accattcgtg ttaattcttg agcgagagtt ttttgagcta
      541 agcgtttttc agggctgttt ttaacttgtt tttctaactc attaatttct tcttgggaaa
      601 gaaaggaaaa atatttaaga aatccaatta cgtcatcatc gcttacgtta ataaaatatt
      661 gataaaattg ataaacagaa gttttttggg aatttaacca aaccgttcct ccttcggttt
      721 ttccaaattt tgtgccatct gattttaata aaagaggcaa ggtaagacca taaactgttt
      781 tattttttgt tttggcaatt aaatcaatgc ccgaagtaat attgccccat tgatcactgc
      841 cgccaatttg cagttcgcaa ttatagtgct cattaagcca taaataatca taggattgaa
      901 gaagcatata gctgaattca gtgaaagaaa tacctgattc caagcggctt ttaacagatt
      961 ctttagccag catatacccc aaactgaaat gttttccaat gtttctaata aaaggtaata
     1021 attttaattc tttgagccat tggttgtttt gcagaatggt tgctttttgg gcgtggattt
     1081 cttcgtcaaa gattttttct agttgttttt ttaattcaat agtccattgt tccacttgct
     1141 cttcagaatt taattggcgt tcggattttt ttccgctggg atcgccgatt aagctcgttc
     1201 catctcccaa cagaggaatg atctgatggt taaattgagc aaagcgtttg agagtaatta
     1261 ttgggagaag gtgtccaata tgcaaactgt cagcagtaac atcaaaaccg caatacaaac
     1321 tgattggttg agtaagtcga tttttcaatt tattagcgtc ggttgtttga ttaattaatc
     1381 cgcgccagga aagttcatcc caaacatttt gcttcatacc attttatttt aatttttttt
     1441 gaaaaataag tcaaaaaaat ccctgatcag ttgaccaggg attctatttc gttttggatt
     1501 aaatgcataa aagcatttaa tccttctttt tctagcaaat ggtcaagaat taatcgtttc
     1561 cttggaatag tattcagctt ctcgtaaatg tttatgttct ttttttcagc ttctttttca
     1621 aaaagcaagc atttcttttt tgcttgaatc aattcggcca acttaagcga tctagtgcga
     1681 tctatatacc agagaccatt gataagttga aatgctccat agggttgaat ttcgtaatgg
     1741 ttagaaatca tcaattttct tacgtacgaa aaaggtggaa tatagtcatt atggattgtg
     1801 attcctgctt caataaaaga attatgtcca ataacagctg ggccgataat ggtaacatta
     1861 gctccgagat atgtcttttt ctcaataaca atcggttcac gaccttggcc agtagagctg
     1921 acaagcttta ctccgctttg aagtgcagag aattcatcta cgcgactaaa atcaacaaca
     1981 acttgaggaa aaagagtaac attatttcca ataatgctat cttcaataga acaaccattg
     2041 gaaatagtca ccccattttt aattcggcta tcgctaatgg tactattgtt tacgtagctg
     2101 ttgttgccca aagaggtttg ctcaaaagtt gaattgatca caacactttt ttggcctata
     2161 atcgcatgga agagtcggct atcgagcaca cgactttttt ctttgattga gcaatgttct
     2221 aaaaagctgt gagacacaaa gcaatgtcga tgaatctctt gtggtccttg gaaaatcacc
     2281 ggagggataa aagccgtatc ttcgccaata aaaattaagg gaaaatcatc cggagagatt
     2341 tcatcagcaa ctctatctcc aaatagcaaa taataaagca ggtcgtctct ttcggcatac
     2401 aaaaaatcta gaagcggaaa aactcttttc aaatccattt tttacctcct ttatgtaggt
     2461 tttaagcaac cacttttaaa ttaaactttt ttaatttttt gtcaatggga taaatttact
     2521 agattataaa aattataaaa aagcaaagaa ttgcaaaaaa atcaaaaatt gataagatat
     2581 aaaatatcat ttgaagtata gcaaaaggtc atttttctgt ttggaaaggg agtttaataa
     2641 tgtgaaaaat gtgggatggg cctgtagctt agctggtaga gcgctgcatt cgcattgcag
     2701 aggtcagggg ttcgagtccc ctcaggtcca ctatatagga catgaagcaa gccaactgct
     2761 tggcttgcgt ggggacgaga agcgcgcagc gttaggcgag ttttccaacc aaaggttgga
     2821 aaggcgagcc tcgcgagcgg cggcctgcga gaatttgccg tcaggcaaat tacttgtagg
     2881 agagtcccct caggtccact a
//
LOCUS       DAOSMC010000038         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_37684_length_2878_cov_25.293659, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000038 DAOSMC010000000
VERSION     DAOSMC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37684_length_2878_cov_25.293659"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PL075_02740"
     CDS             complement(<1..1482)
                     /locus_tag="PL075_02740"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HOD97122.1"
                     /translation="MDILQYMGSLEIDAKDEEQLSKFLNEKESEIKDFIKTSWQNFSM
                     VNTNTIADDDAIKINVGLEEETETNEDSKIDAENETIITSDKNEPALIQNENTIIAES
                     KTEPEPLKVEQIKDKSSNSFELQIKNKSIIIPNGKVNQDYCFLFKIEELGLTEIGDFW
                     FEGLEPIGLIFNSDTNEIKGNPNTAGDHKIKLKIKRKDWSDGKPVFERQITFIINPDP
                     KSLWKNIPTPNDIEYYKPDSDKCFVKVTSSTNIFGLGKTHKKDIVAASQRGRSHAQEG
                     KPRDDDFNIRYISEVDWYILSVADGAGSAKSSRKGSEIACKTVVEVCEQLIKEKTKEF
                     ENLIKDFSTEKSDERRKKMGDSLYGILGNSVFKAVKNIEEEAKKTNKPLKDYSTTLIV
                     SICKKFKFGWFVGAFWVGDGGIGIYNKDTNFLKILGESDGGEYAGQTRFLTMPEITQP
                     TELYRRLRFDIVDDFTALILMTDGITDPKFETDANLLKVEKWNE"
     gene            complement(1485..2534)
                     /locus_tag="PL075_02745"
     CDS             complement(1485..2534)
                     /locus_tag="PL075_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerY-C metal binding domain-containing protein"
                     /protein_id="HOD97123.1"
                     /translation="MRRLPIYFVIDVSESMIGEPIEQVQEGIATIIKELRTDPYALET
                     VYVSIIVFAGKVQKITPLIELFNFYPPKLPIGGGTSLGNALNFLMNDFDSSLQKTTPE
                     IKGDWKPIIFLFTDGNPTDKYETAFDKWNQKYKSKSNLVVISLGDNTDTSIFGKITDQ
                     VLLLKNTDSESFKKFFKWVTASIKTSSVSVSESKDDGLHLAPITNGYLSKIDLEKGFN
                     KKVDENFAVILAKCQSTKRPYLIKYQKRISPSEFKELALNTVVYKLVGAYPVENSYFD
                     LCENDSNQIKINTSELVGFPACPCCGNQFGFSHCGCGNIMCTGEEEVSKCPWCGIEAK
                     FGFGEGSININRTRG"
     gene            complement(2531..2788)
                     /locus_tag="PL075_02750"
     CDS             complement(2531..2788)
                     /locus_tag="PL075_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOD97124.1"
                     /translation="MRLFLYILVNVLIVGLFLYSKLTPYKNRLTGRYLKAFNFFESIF
                     NPFLNFLKKIAKPAQVGNGIAVDMSQIILLLLLLLTLKFIL"
BASE COUNT          874 a          524 c          415 g         1065 t
ORIGIN      
        1 ttcattccat ttttcaactt tcaacaaatt tgcatctgtt tcaaatttcg ggtcagtaat
       61 tccgtcagtc attaaaatta atgctgtgaa atcatcaaca atatcaaatc tcagtctcct
      121 gtataattct gtgggttggg ttatctcagg cattgttaaa aaacgtgtct gacctgcata
      181 ttcaccacca tcagattctc ctagaatctt taaaaaatta gtgtctttgt tgtaaatacc
      241 aataccacca tcaccgaccc aaaatgctcc aacgaaccat ccaaatttaa atttcttaca
      301 aattgatact attagagtcg tggaataatc ttttaaaggt ttgttagttt tctttgcttc
      361 ttcttcaatg ttttttactg ctttaaacac tgaattacca agaatgccgt acaaagaatc
      421 tcccattttt tttcttctct catccgattt ttcagtgcta aagtctttga ttagattttc
      481 aaattctttc gttttttcct taattagttg ttcgcaaact tcaactactg ttttacatgc
      541 tatttcagaa ccttttcttg aggatttagc agaaccggca ccatcagcaa cggataaaat
      601 ataccaatca acttcactta tatatctgat attaaaatca tcatcacggg gtttaccttc
      661 ttgggcatgt gaacgacctc tttggcttgc agcaacaatg tcttttttat gggtttttcc
      721 tagaccaaat atattggtgg aggatgtgac ttttacaaaa catttgtcgc tatcaggttt
      781 atagtattca atgtcatttg gagtaggaat atttttccat aatgatttag ggtctggatt
      841 aattataaat gtgatttgtc tttcgaagac cggtttccca tctgaccaat cttttctctt
      901 aatttttagt tttattttgt ggtcgccagc agtgtttggg ttgcctttaa tttcatttgt
      961 gtcagaatta aaaattagcc caattggctc taaaccttca aaccaaaaat cgccaatttc
     1021 tgttaaaccg agttcctcaa ttttaaatag aaagcaataa tcttgattta ctttaccatt
     1081 tggaataata atgcttttat tctttatctg taattcaaag gagttagaac ttttatcttt
     1141 tatctgttca acttttaagg gttctggttc tgttttactc tctgcgatta tggtattttc
     1201 gttctgtatt aatgctggct cgtttttatc ggatgttata attgtttcat tttctgcatc
     1261 aatttttgag tcttcgtttg tttcagtttc ttcttctaac cctacattta tttttatagc
     1321 atcatcgtca gcaattgtat tagtgtttac catagaaaaa ttttgccatg atgtttttat
     1381 aaagtctttt atttctgatt ccttttcgtt aaggaatttt gataattgtt cttcgtcttt
     1441 tgcatctatt tcaagactac ccatgtattg taaaatatcc ataactaccc ccttgtcctg
     1501 ttaatattaa tactaccttc tccaaaacca aattttgctt cgatgccgca ccaaggacat
     1561 ttactaactt cttcttcacc cgtacacatt atatttccac agccacaatg actaaaacca
     1621 aattgattac cacaacaagg gcaagcagga aaaccaacta attcagaggt gtttattttg
     1681 atttgatttg aatcattttc gcacaaatca aagtaagaat tttcaacagg atatgcacca
     1741 acgagtttat aaactaccgt gtttaatgct aattctttaa attcggatgg tgaaattctt
     1801 ttttgatact tgattaaata gggtcttttg gtgctttgac acttggctaa aataacagca
     1861 aaattctcat caaccttttt attaaaaccc ttttcaaggt caatttttga taaataacca
     1921 tttgtaattg gtgctaaatg caagccatca tcttttgatt ccgatacact tacactactc
     1981 gttttgatag aagctgttac ccatttaaaa aacttcttaa atgattcaga atctgtattt
     2041 ttaagcaata gcacctggtc ggtaatttta ccaaatatgc tcgtgtcagt gttgtcacct
     2101 aatgagatta ctacaagatt ggattttgat ttatattttt gattccattt atcaaaggct
     2161 gtttcatatt tatctgtagg attaccatca gtaaataaga aaataattgg tttccaatct
     2221 cccttaattt cgggagtagt cttttgaagt gacgaatcaa aatcattcat taaaaaattc
     2281 agagcatttc ccaatgaagt acctccacct attggcaatt tgggcggata aaaattaaaa
     2341 agctcaatta aaggtgttat cttttgcact ttacctgcaa aaactattat tgaaacgtaa
     2401 acagtttcca acgcataagg gtcggttctt agttccttaa ttatagttgc aataccttct
     2461 tgaacttgtt caatgggctc gccaatcatt gactcagaaa catcaattac aaaatatatg
     2521 ggtaatcttc tcataaaatg aattttaatg ttaataatag caacaagagc aaaattattt
     2581 gcgacatatc aacagcaatg ccattgccaa cttgggcagg tttagcaatt ttctttagaa
     2641 agtttaaaaa cggattaaaa atactttcaa aaaaattgaa ggcttttaag taccttccgg
     2701 ttagcctatt tttataaggt gtaagtttag aatacaggaa taaacctaca atcaatacat
     2761 taactaatat gtataaaaat aatctcacaa caaattatat tacagaatga acttccggag
     2821 gtggcggtgg taacaacatt tggtcagtag caccaatact tctgttgcca acactaac
//
LOCUS       DAOSMC010000039         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_53277_length_2216_cov_19.864415, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000039 DAOSMC010000000
VERSION     DAOSMC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53277_length_2216_cov_19.864415"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1734
                     /locus_tag="PL075_02755"
     CDS             82..1734
                     /locus_tag="PL075_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HOD97125.1"
                     /translation="MADYREENVNIFAETNFRNQRTKFGIKLDDRRKHMYAIGKTGMG
                     KTVMLENMAISDIESGFGLAFIDPHGESAERLLNFIPENRIKDVIYFCPHDTDYPIAF
                     NPLETVGEEHRHLVASGILEIFKKIWPDVWSARMEYILNNCLMALLEYPNSTLLDIMR
                     LLSDKKYREYIISGLTDPVVKNFWLNEFSRYHDKFQVEAIAPIQNKVGQFLTNPLIRN
                     IVGQPQSSFNIRQIMDEGKILIVNLSKGRIGETNSALLGAMIVTKIQQAAMSRADIPE
                     KDRRDFFLYVDEFQTFATEAFANILSEARKYHLCLIISHQYIAQLPDTVRAAIFGNVG
                     TFIVFRVGAEDAEWLEKEFAPEFMANDLVNLGKYNIYLKLMIDGITSKAFSAVTLPPR
                     PIPEITFVKEIIENTRQIYCRPRAEVEKMISEEWAPIETLDLKEEKVKIEKPKKKTFV
                     DFCWVCGKETEVPFEPDGKRPIYCKDCLRGVEAGEIIPPLKPLHLETKVVKALESHAK
                     KIAEKKPNEKPKLKVDVDELKKALRESLSKLSNQEEKKEGDH"
     gene            1752..1970
                     /locus_tag="PL075_02760"
     CDS             1752..1970
                     /locus_tag="PL075_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOD97126.1"
                     /translation="MRVIVKVKPGASFNKIIQIDSNQFEIFTTASAVKGKANKAVIEI
                     LAEFFDVAPSRISLVKGAKTKQKTFEIN"
BASE COUNT          727 a          383 c          442 g          664 t
ORIGIN      
        1 ataggaaata gactttcact atattataaa ctcatacatc gatttgagtt tttttacttt
       61 tgcgttacaa taaagtggta tatggctgat tatcgagagg aaaatgttaa tatttttgcg
      121 gaaactaatt ttcgcaatca acgaaccaaa tttggcatta aattagatga ccgacgcaaa
      181 cacatgtatg ctatcggtaa aacaggcatg ggcaagacag tgatgctaga gaatatggcg
      241 atttcagata ttgaatcagg atttggttta gcttttattg atccgcacgg cgaatcggcc
      301 gagcgtttgc ttaattttat tccagaaaat cgcattaaag acgtgattta tttttgtcct
      361 cacgatacag actatcccat tgcttttaat cccttggaaa ccgttggtga agaacaccgc
      421 catttggtgg cgagtggcat tttagaaatt ttcaaaaaga tttggcctga tgtttggagt
      481 gctcgtatgg aatacatttt aaacaattgt ttgatggcct tgcttgaata tccaaactcc
      541 acacttctag acatcatgcg tcttttatcc gataaaaaat atcgcgagta tattatttca
      601 ggattaaccg atccagtggt taaaaacttt tggcttaatg aattttcgcg ttatcatgat
      661 aaatttcaag tagaagccat tgctccaatt caaaataaag taggtcaatt tttaaccaat
      721 ccattaatta gaaatattgt tggtcagccc caatcatcat ttaatattcg tcagatcatg
      781 gatgaaggaa aaattctcat tgtgaatcta tctaaaggaa gaattggaga aacaaattca
      841 gcgcttttgg gcgcaatgat agttacaaaa attcagcaag ccgcaatgag tcgcgctgac
      901 attcccgaga aagaccgtcg cgatttcttt ttatacgttg atgaatttca gacttttgcc
      961 accgaggcct ttgctaatat tttatccgag gctcgtaaat accacctttg tttaattatt
     1021 tcgcatcagt atattgctca attgcccgat acagtgaggg cagctatttt tggcaacgtg
     1081 ggcactttta ttgtttttcg cgttggagct gaagatgctg aatggttaga aaaggaattt
     1141 gcgccggaat ttatggctaa cgatttagtt aatttaggta aatacaatat ttatcttaaa
     1201 ttaatgatcg atggtatcac ttccaaagca ttttcggccg tgacattgcc accgcgacca
     1261 attccggaaa taacttttgt taaagaaatt attgaaaaca ctcgtcaaat atattgtcgg
     1321 ccacgcgcag aagtagaaaa aatgattagc gaagaatggg cgccaataga aacattagac
     1381 ctaaaagaag aaaaagttaa aattgagaag cccaaaaaga aaacttttgt tgatttttgc
     1441 tgggtttgcg gcaaagaaac cgaagtgcct ttcgagcccg atggcaaaag gccaatttat
     1501 tgcaaagatt gcttgagagg tgtagaagcg ggcgaaatta ttccgcccct caagccgctc
     1561 catttagaaa ccaaagtagt taaagcatta gaaagtcatg cgaaaaaaat agcagagaaa
     1621 aagccgaatg aaaaaccgaa attaaaagtt gatgttgatg aattaaaaaa agcgcttcgt
     1681 gaatcgcttt ccaaattgag taatcaagaa gaaaaaaaag aaggcgatca ttagaaatag
     1741 gccttggatt tatgcgagtg attgttaaag taaaacccgg cgcttctttc aacaaaatta
     1801 ttcaaattga tagcaatcaa ttcgaaattt ttacgactgc ttcggcagta aagggtaaag
     1861 ccaataaggc cgttatagaa atattagcag aattttttga tgtagcacct tcgcgaatat
     1921 ctttggttaa aggcgctaaa actaaacaaa aaacttttga aattaattga cacgttgcaa
     1981 atatttttta agatagaatg caatattaat caaagaaaca gcatcttgtt attccgaata
     2041 ttttttgtca ttctgagcat tttatttctg tcattctgag ggcccaccag gggtgggcag
     2101 gcgcagcccc ccgcctaacc ggcgggcagg gaagaatccc ccgaaaagct ggcatgagat
     2161 ttttcggact atatcctttt aacttttgtc tatgttagga atcaatgatt taaaac
//
LOCUS       DAOSMC010000040         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_58016_length_2081_cov_20.524679, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000040 DAOSMC010000000
VERSION     DAOSMC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58016_length_2081_cov_20.524679"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..989
                     /locus_tag="PL075_02765"
     CDS             240..989
                     /locus_tag="PL075_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOD97127.1"
                     /translation="MRLENKIAIVTGAGSGIGRGIALTFTKEGAKIVVADYAEAGGKA
                     TVEEIKKMGGEAIFVKADVSKADEVKNIFNETMKNFGRVDILVNNAGIFRTGNFHEMA
                     EQDWDAIININLKSMFLTIKEVAPIMLKQGKGKIINIASIAGLVGFPLSGAYCASKGG
                     VIALTRELSMEYAPKKINVNAIAPGVIQTAMTKDMINDPNTKKYLESSTLYPRLGEPN
                     DIANAALYLASDESDFVNGEVLVVDGGWVAQ"
     gene            1001..1828
                     /locus_tag="PL075_02770"
     CDS             1001..1828
                     /locus_tag="PL075_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOD97128.1"
                     /translation="MDLKKIYLQRNRWSHKGQFGYVLIIAGSELYTGSPIFNSLAALR
                     SGADLVTVITPYRTANVASAFLPDIISYPLNKNLSREHIPVIIRLSKKFNSLIIGGGL
                     NRDKDTFNVVRELIKKIDLPMVIDAEAIRALNQHQQILKNKTAVMTPHKEEFRILTGE
                     TVADDIADRKIKVKKWAQKLHTVILLKGHADVISDGKKTVINKTGSPYMTKGGFGDTL
                     AGVCGALLARNINPFQAAVAAAFINGKAGELAAQKYGESVLASDVFDFIPQVIKKFA"
BASE COUNT          654 a          375 c          434 g          618 t
ORIGIN      
        1 aatacgcaac tattactgca ttgcgtggga ttcttcgctg acgctcagaa tgacagaaaa
       61 caaaaacgct cagaatgaca acaataaaat aatcagaatg aagcgagact gcaaaattgc
      121 caaaagcaat tttgcagtct cgcttcataa ttttaacttt taaattttta tttgcatctc
      181 ttttcgctat tagttataat taaattaaat aattaaggtc gtctaaaaaa ttcaataata
      241 tgcgtttaga aaataaaatt gcgattgtca ctggcgccgg atcaggcatt ggtcgcggca
      301 ttgctcttac ttttaccaaa gaaggagcaa aaattgtagt ggctgattat gctgaagctg
      361 gcggcaaggc cacggttgaa gaaattaaaa aaatgggcgg agaagccatt tttgttaaag
      421 cagatgttag caaggccgat gaagtgaaaa atatttttaa cgagacgatg aaaaattttg
      481 gccgtgttga tattttagta aataatgccg gcatcttccg cactggtaat tttcatgaaa
      541 tggcagaaca agattgggat gcaataataa atattaattt gaaaagcatg tttcttacca
      601 ttaaagaagt cgccccaata atgttaaaac aaggtaaagg aaaaattatt aatatagctt
      661 ctattgctgg cctcgttgga tttcctttaa gcggcgctta ctgcgcttca aaaggtggcg
      721 taattgctct cactagagaa ttatcaatgg agtatgcgcc aaaaaaaatc aatgttaatg
      781 ccattgcacc gggcgttatt caaacagcaa tgacaaaaga tatgattaat gatccgaata
      841 caaaaaaata tttagaaagc tcaacacttt atcctcgctt gggcgaacca aatgatattg
      901 caaatgccgc gctttattta gcttcagatg aatcagattt tgtgaatggc gaagttttag
      961 tagtagatgg cgggtgggtt gctcaataaa tgcggttgat atggatctca aaaaaattta
     1021 tctccaaaga aataggtgga gccataaagg tcaattcggc tatgttttaa taatagctgg
     1081 cagcgaactt tatacgggct cgcctatttt taattctctt gccgctttgc gcagtggagc
     1141 tgatttagtg actgtcatta ctccttacag aactgctaat gttgccagcg cctttctgcc
     1201 agatattatc agctaccctt taaataaaaa tctcagtcgg gaacatattc cagtaattat
     1261 cagactcagc aaaaaattta atagtttaat tattggcggg ggtttaaatc gcgataagga
     1321 cacattcaat gttgttcgag agcttattaa aaaaattgat ctgccaatgg tgattgacgc
     1381 tgaggcgatt cgcgctttga atcaacatca acagattctt aaaaataaaa cagcagtgat
     1441 gacaccacac aaagaagaat ttcgtatttt aaccggtgaa acagtagccg atgatattgc
     1501 tgatcgcaaa ataaaagtta aaaaatgggc tcaaaaattg catactgtta ttctattaaa
     1561 agggcatgct gatgttatct cagacggaaa aaagacagtg attaataaaa ccggctcgcc
     1621 ttatatgact aagggtggtt ttggtgatac tttagctggt gtgtgcggtg ctcttttggc
     1681 aagaaatatt aaccctttcc aagctgctgt tgccgctgcg tttattaatg gaaaagctgg
     1741 cgaactagca gctcaaaaat atggcgaaag tgttttagcc agtgatgttt ttgatttcat
     1801 tccgcaagta attaaaaaat ttgcttgata catttttccc ttttgccatc ctatttttta
     1861 ttgtcatcct ttttttcggc gtcatcctga acggagcctg cccgccctgc ctaccgcagg
     1921 caggcgaaca ggcgggtgaa ggatcccatg gcttttagca ctcttttgtc atcctgaggg
     1981 taacacccga aggatcccat ggcttttagc aatgcgcaac aatcactgct gtggatgaga
     2041 ttcttcggac ttcgtcctca gaatgaccgt ggggattttg c
//
LOCUS       DAOSMC010000041         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_59959_length_2030_cov_18.841013, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000041 DAOSMC010000000
VERSION     DAOSMC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59959_length_2030_cov_18.841013"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1448)
                     /locus_tag="PL075_02775"
     CDS             complement(165..1448)
                     /locus_tag="PL075_02775"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOD97129.1"
                     /translation="MFKQDYQQVSHKPKIFWINLSQRSNEIFCDFVFSHLYLKPKDRK
                     CAHLLEHYLVALLKEQCSNSSVEIKGFVDAESIVIHFGFRVKTFLNDLSQCLSLFSQC
                     NFQRHDIFSSEKRAVINEFVENQDKLSNQIGHRIINAKPFQNCPYFQSGVYSIQTIKK
                     IGLQDIQNLYRQLYQPVCFIGAHQFHKRLVFKGRNPISRVFGRFPSIPVKGHDFQHCK
                     INQFQFETLCTKNIPANKIYGLFIFPTFEIKKENLIQRISLNILCSLLFENSRNTLFK
                     RLRKEGVYSIYYANQFFHHAGFTFILFLSDASKFLKTCQIFKEEIEFNKHNSSIINEK
                     EIRRSIKEIQQSQKIIWKDNQKKFNFIKENLLTEKRVIDQRIYASISHQIDSQLLKEI
                     TKKVFDWHQFFVLLVFNNNKPKKLDIYKIFKDRLN"
BASE COUNT          678 a          349 c          302 g          701 t
ORIGIN      
        1 ttcttaaaga aaatccccgc cgtatatcat cctaagtgtt tcttatcatt ctgagatctt
       61 gcccgccggt tagacggaca aaggattccc cgaaaagatt gtatggaatc ctcctcttca
      121 ctcatgataa caatgaaagt aaaaactgaa ctttcactac gtttttaatt caatctgtcc
      181 ttaaatatct tataaatatc tagttttttg ggtttgttat tattaaaaac gagaagaaca
      241 aaaaactgat gccaatcaaa aactttctta gttatttcct tcaataattg actatcaatt
      301 tgatgagaaa tacttgcata aattctttga tcaataactc gtttttctgt taataaattc
      361 tctttgatga aattaaattt tttttgattg tctttccaaa ttattttctg agattgttgg
      421 atttctttaa tactccggcg gatctctttt tcattaataa tactagaatt gtgtttatta
      481 aattctattt cttctttaaa aatttggcag gtttttaaaa attttgatgc gtctgataga
      541 aacaaaataa atgtaaaacc agcgtgatga aaaaattgat tggcataata aattgaataa
      601 acaccttctt ttcttaatcg tttaaatagc gtattccgag aattttcaaa taataaacta
      661 cataaaatat ttaagcttat ccgttgaatt aaattttctt ttttaatctc aaaagtggga
      721 aaaataaata atccataaat tttatttgca ggaatgtttt ttgtacaaag agtttcaaat
      781 tgaaattgat taattttgca atgctgaaaa tcatgaccct tgactggaat agatggaaat
      841 cgaccgaata ctcgagaaat cggattgcga cccttaaaaa caagccgctt atgaaattga
      901 tgagctccaa taaaacaaac gggttgatat aattgacgat aaagattttg aatatcctga
      961 agtccgattt ttttgattgt ttggatagaa taaacgcctg attgaaaata ggggcaattt
     1021 tggaagggct ttgcatttat aattcggtgt ccaatttgat tagataattt atcttgattt
     1081 tcaacaaatt cattaataac tgctcttttt tcactagaaa aaatgtcatg ccgctgaaaa
     1141 ttacattggg aaaatagact tagacattgc gataaatcat tcaaaaatgt tttaactcta
     1201 aaaccaaaat gaataacaat cgactcagca tcaacaaatc ctttaatttc tacagaacta
     1261 ttactgcatt gttctttcaa aagtgctacc aaatagtgct ccaataaatg ggcacatttt
     1321 ctatctttcg gctttaaata aagatgagaa aaaacgaaat cgcaaaaaat ctcatttgaa
     1381 cgttgagaaa gattaatcca aaaaatcttt ggtttatggg aaacttgttg gtaatcctgt
     1441 ttgaacattc gttttattat accttgtaac ccaataattg caaaaagaaa agagggcgat
     1501 gaaccctctt taatataatt taatatactt catcaaattt aatcatcttt attagtccca
     1561 aaaattttgg acaaaatatt tgagaggatg ctcctattcc gcggatcgtc tttactaatt
     1621 tctgtcccat aaatatggac tttcttcccg gttgaatcat aggaatccca accaaccgaa
     1681 ccgtcccgat gctgatgaaa tttactcaaa gaacccgttt tatcattgga attatggtct
     1741 ttccccattc cttaatcacc tcctttgtct tgcataaatt tgtccagttc tgaataccgg
     1801 acagcaagac atatagatcc ttattcatct ttttaagaat ctatttccat tatactcccc
     1861 atttttcttt tgtcaaatgc cagaaagaat taaaaagtga atggttggat tgacacgctg
     1921 tggcgatttt ctttaagaaa atcgccacag cgtgtcattc ttggggcttt tccaactgtc
     1981 attctgagga ccaccaaaga gggcgggctt caacccctgc ctaccggcag
//
LOCUS       DAOSMC010000042         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_1607
            NODE_88523_length_1533_cov_20.456022, whole genome shotgun
            sequence.
ACCESSION   DAOSMC010000042 DAOSMC010000000
VERSION     DAOSMC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863136
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:50:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 557
            CDSs (total)                      :: 507
            Genes (coding)                    :: 505
            CDSs (with protein)               :: 505
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88523_length_1533_cov_20.456022"
                     /isolate="AMR_MDS_1607"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..900)
                     /locus_tag="PL075_02780"
     CDS             complement(187..900)
                     /locus_tag="PL075_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97130.1"
                     /translation="MYSESFRIFVREGLDKYFIGTGNPNAKILFIGKESAIAPTDTVG
                     RNWYLKNATEWKCHLDNDTEECYSYTVDENHPLRKNWGKNTWSKYQLLSDYIWNKQTE
                     KYRVEFLQSIFTTEINDSPAKTTSAANKSGLNERKLLFKNSTFIQEFPVIVLACSDYI
                     KNNSETREIDEIFNVVYDGDEKGKYWFNKTNWFFTHHNPDKSKLVIHTRQLSADVSNE
                     MLKIMGEVIRQHLIKTEKF"
     gene            complement(904..1479)
                     /locus_tag="PL075_02785"
     CDS             complement(904..1479)
                     /locus_tag="PL075_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOD97131.1"
                     /translation="MGKVSDSFKRQIGRDAGKVVSNIIFGDKHSTPYRRVESKKQKAE
                     LEDKKMKIIQEKAENDDLYALDRAVNSSVDQIVAIQMPDNERAIVNLLNSLETLLESN
                     SWEPEKDKEKKKAKIRNKYPDAVLNKFEQVLRELKFINANSERISFYQNRIDTFKKIQ
                     KKAKKAEVRQNIIIVTVILFIFLILIIASAA"
BASE COUNT          439 a          267 c          219 g          608 t
ORIGIN      
        1 tctctatgaa ttccattcgt ttccgatggt tttgtagcat tacgcccaac ggtcggtggt
       61 ttgggctgct ggggagtaaa tagccagcgg cgtcatccca cgttaaaact atactaaatt
      121 actaaaaact ttcgtatctg catcatcagc cccagtaggc taaaccactt gttagcgttt
      181 cgtgcattaa aatttttctg tctttatcaa atgttgtcta attacttcgc ccataatttt
      241 caacatttca ttactcacat cagcacttaa ttgtctagtg tgaattacaa gttttgattt
      301 gtcaggatta tggtgtgtaa agaaccaatt ggttttatta aaccaatatt ttcctttttc
      361 atctccatcg taaactacat taaaaatttc gtcaatttcc ctcgtttcag agttattttt
      421 aatgtaatca gaacaagcta atactataac tgggaattct tgaatgaagg tagaattttt
      481 aaacaataat tttctttcat ttaatccact tttattagct gcagaagttg tctttgctgg
      541 tgaatcatta atttcagtag taaatattga ttgtaaaaat tcaactctgt acttttcagt
      601 ttgtttattc caaatgtaat ctgaaagtaa ttgatattta ctccaagtgt ttttacccca
      661 attttttctt aaaggatggt tttcatccac agtgtaggag tagcattctt ctgtatcatt
      721 atcgagatga catttccatt cagtagcatt ttttagatac caattcctgc ctacagtatc
      781 ggttggggct attgcagatt ccttgccaat aaaaagaatt tttgcatttg gatttcctgt
      841 tccaataaaa tacttgtcaa gaccttctct gacaaaaata cggaatgatt cactatacat
      901 aatctatgca gctgatgcta ttattaaaat taagaagata aaaagtatta ctgtaacaat
      961 aataatattc tgtctaactt ctgctttttt tgcttttttc tgaatttttt taaacgtatc
     1021 aatcctgttt tgataaaaac ttatacgttc agaatttgca ttaataaatt tgagttctct
     1081 taggacttgt tcaaatttgt taagaactgc atcaggatat ttattcctta tttttgcttt
     1141 tttcttttct ttatcttttt ctggctccca gctattagat tctaaaagag tttctaaact
     1201 gtttaataaa ttaacaattg ctctttcatt atctggcatt tgaatagcaa caatttggtc
     1261 tacagaagaa ttaacagctc tatctagagc atacaaatcg tcattctcag ctttttcttg
     1321 aattattttc atttttttat cttcaagttc ggctttttgt tttttcgatt caactcttct
     1381 gtaaggagta gaatgcttat ctccgaaaat gatattggaa acaactttgc ctgcatctct
     1441 tccaatttgc cttttaaatg aatccgatac ttttcccatt attgtaaagt ttaattgtta
     1501 tatgaatcgt ctctttgcat gaacgctaac ggt
//
